LOCUS       DASVLN010000001         5962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_53786, whole genome shotgun sequence.
ACCESSION   DASVLN010000001 DASVLN010000000
VERSION     DASVLN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53786"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..641
                     /locus_tag="VF453_00005"
     CDS             108..641
                     /locus_tag="VF453_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7686048.1"
                     /translation="MSATTAIEHRAAAQAANAAVHGAAREYLSFKLGAEEYGIDILKV
                     QEIRGYENPTRIANAPHFIKGVVNLRGVIVPIVDMRLRFGLAEVQYNSFTVVIILNIG
                     SRTVGMVVDSVSDVLELGGEQIKPAPEFNGAIDAAYITGLGTIKTGDTERMLILMDIE
                     QMMTAPDMGLMDDAISH"
     gene            complement(774..1397)
                     /gene="lolA"
                     /locus_tag="VF453_00010"
     CDS             complement(774..1397)
                     /gene="lolA"
                     /locus_tag="VF453_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEX7686049.1"
                     /translation="MMTLSTTFRAALFAAAATLAAHAQASATDDLREFVKTVKTGRAT
                     FSQTVTSPQGKKKQSRGSFEFERPNRFRFVYEKPYAQTVVGDGTRVWLYDPDLNQASS
                     RKMGEALGATPAALLVSTDIEHEFTLAEEPAKDGLQWVQATPKQADGTIRWLRLGFKE
                     HALAQLEIADSFGQDSLLVFDGFQPNVPVPAADFTFTPPKGADVVEQ"
     gene            complement(1394..3625)
                     /locus_tag="VF453_00015"
     CDS             complement(1394..3625)
                     /locus_tag="VF453_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX7686050.1"
                     /translation="MQLVAWTAVAMALASHRPTDPGFTTSGDGGPVLNLLGRPGAWLS
                     DALLAAFGGSVAWLPLVGLLQALRGLARSSRSLSAGREDGPVARWPRLRFWGGLVLLM
                     CASTALEWTRLYTWEPRVPGGHSGGVLGFVLGPPSMHWLGFVGSGVFWIAALVVGMAG
                     ALRFSWVALADGIGAWIDGRREIRAVQREREEDLRIGEIALREREHAADDVRVDDVPH
                     VPIVIEPEVVQVPESKRVARERQKPLFAEIADNRLPQVDLLDVPPSRVETVTPESLEM
                     TSRMIEKKLGDFGVEVRVVAAAPGPVITRYEIEPATGVKGSQIVNLAKDLARSLSLVS
                     IRVVETIPGKTTMALELPNARRQTIRLSEILGSTVYNEAGSLLTMGLGKDIVGNPVVA
                     DLAKMPHCLVAGTTGSGKSVGINAMILSLLYKAEGRDVRLILIDPKMLEMSVYEGIPH
                     LLCPVVTDMKQAGNALSWCVNEMERRYKLMSKMGVRNLAGYNKKIDEAAERGEKIPNP
                     FSLTPEDPEPQERMPFIVVVIDELADLMMVVGKKIEELIARLAQKARAAGIHLVLATQ
                     RPSVDVITGLIKANIPTRLSFQVSSKIDSRTILDQMGAEALLGQGDMLYLAPGTGLPT
                     RVHGAFVSDDEVHRVVEYLKSQGPANYIEGILEGGVLDGELDGDGTGGGSADAEADPL
                     YDQAVAVVLQNKRASISLVQRHLRIGYNRAARLLEQMEQSGLVSGMSGSGNREILVPK
                     RDE"
     gene            complement(3885..4547)
                     /locus_tag="VF453_00020"
     CDS             complement(3885..4547)
                     /locus_tag="VF453_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7686051.1"
                     /translation="MSMLSNLDLIRRVPLFSMLTTDQARVVADGVVKRRFRRGELVVE
                     QGKKSNALFILLSGRARVTTSDSRGREVILAVLQAGDYVGEMSLIDNEPHSATVRCEL
                     QCDVLILGRAEFARCLPENSSLSYAIMRGLVTRLRSADRQIESLALLDVYGRVARTLL
                     DMAEDIDGEKVIKSKVSRQDMAKIVGASREMVSRVMKDLEERGIIETQEDGSLKIQNR
                     KS"
     gene            4694..5659
                     /gene="trxB"
                     /locus_tag="VF453_00025"
     CDS             4694..5659
                     /gene="trxB"
                     /locus_tag="VF453_00025"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX7686052.1"
                     /translation="MTSPANDSNHAQVLILGSGPAGYTAALYAARANLRPMLVTGLAQ
                     GGQLMTTTEVDNWPADVAGVQGPDLMARFLAHAERFDTKIVFDHINKVDLSRRPFTLV
                     GDSGTYTCDALVIATGASAKYLGLPSEESFMGRGVSGCATCDGFFYRGEEVCVVGGGN
                     TAVEEALYLANIASKVHLIHRRNKFRAEPIMIDKLMQKAAEGRIQLHLDQTLHEVLGD
                     QTGVTGVRTKSVEGEAYAEIAVKGCFIAIGHSPNTSIFEGQLKMKDGYIVTRSGNEGF
                     ATMTSVEGVFAAGDVQDHVYRQAITSAGTGCMAALDAQRFLEQFA"
     gene            5835..>5962
                     /gene="rpmB"
                     /locus_tag="VF453_00030"
     CDS             5835..>5962
                     /gene="rpmB"
                     /locus_tag="VF453_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX7686053.1"
                     /translation="MARVCQVTGKRPMVGNNVSHANNKTKRRFLPNLQYRRFWVES"
BASE COUNT          944 a         2124 c         1990 g          904 t
ORIGIN      
        1 atcggcgcat ccgaccgccc ccgcccgccg cacactgcat gccactgacg cgcgccgtcc
       61 ggcccggccg gcggcgcatc cgaacgcagc aaggagccct ttcgcccatg agcgccacca
      121 ccgccatcga acaccgcgcc gccgcccagg ccgccaacgc cgccgttcat ggtgcggcgc
      181 gcgaatacct gtccttcaag ctcggcgccg aggagtacgg catcgacatc ctgaaggtgc
      241 aggaaatccg cggctacgag aacccgaccc gcatcgccaa cgcgccgcac ttcatcaagg
      301 gcgtcgtgaa cctgcgcggc gtgatcgtcc cgatcgtcga catgcgcctg cgcttcggcc
      361 tcgccgaagt ccagtacaac agcttcaccg tcgtcatcat cctgaacatc ggctcgcgca
      421 ccgtcggcat ggtcgtcgat tcggtgtcgg acgtgctgga gctcggcggc gagcagatca
      481 agcccgcccc cgagttcaac ggcgcgatcg acgccgccta catcaccggc ctgggcacca
      541 tcaagaccgg cgacaccgag cgcatgctga tcctgatgga catcgagcag atgatgacgg
      601 cccccgacat gggcctgatg gacgacgcga tctcccacta agaacacgcc ggggcgatcc
      661 aacgccgcga cctcaccgcg tcgcgcacct ggcgccattc ctcgaagttg aaggccgcgc
      721 cgggcaaccg gggcggcctt gtctttttgc ggccgcggac gagctcgccg cgcctactgc
      781 tcgacgacgt ccgcgccctt gggcggcgtg aacgtgaagt ccgcggccgg cacgggcacg
      841 ttgggctgga agccgtcgaa gaccagcagc gagtcctggc cgaagctgtc ggcgatctcc
      901 agctgcgcga gcgcgtgctc cttgaagccg agccgcagcc agcggatcgt gccatcggcc
      961 tgcttcggcg tcgcctgcac ccactgcagg ccgtccttgg cgggctcctc ggcgagcgtg
     1021 aactcgtgct cgatgtcggt cgacaccagc agcgccgccg gcgtcgcgcc gagcgcctcg
     1081 cccatcttgc gcgacgaggc ctggttcagg tccgggtcgt acagccacac gcgggtgccg
     1141 tcgccgacga cggtctgggc gtacggcttc tcgtagacga agcggaagcg gttcgggcgc
     1201 tcgaactcga agctgccgcg cgactgcttt ttcttgccct ggggcgacgt gacggtctgc
     1261 gagaaggtcg cgcggccggt cttgaccgtc ttcacgaact cgcgcaggtc gtcggtggcc
     1321 gaggcctgcg cgtgtgcggc cagcgtcgcg gccgcggcga acagggccgc gcgaaaggtc
     1381 gtcgataggg tcatcattcg tctcgcttgg gaaccaggat ctcgcggttg ccgctgcccg
     1441 acatgccgga caccagcccc gactgctcca tctgctcgag cagccgcgcc gcgcggttgt
     1501 agccgatgcg caggtggcgc tgcacgagcg agatcgacgc ccgcttgttc tgcagcacca
     1561 ccgccaccgc ctggtcatac aacggatcgg cctccgcatc ggctgacccg ccgcccgtcc
     1621 cgtcgccgtc gagctcgccg tcgaggacgc cgccctcgag gatgccctcg atgtagttcg
     1681 cggggccctg cgacttgagg tactcgacga cgcggtgcac ctcgtcgtcg ctcacgaagg
     1741 cgccgtgcac gcgggtcggc aggccggtgc ccggcgcgag gtagagcatg tcgccctggc
     1801 cgagcagcgc ctcggcgccc atctggtcga ggatcgtgcg gctgtcgatc ttgctcgaga
     1861 cctggaacga caggcgcgtc gggatgttgg ccttgatgag tccggtgatc acgtccacgc
     1921 tgggacgctg cgtcgcgagc acgaggtgga tgccggccgc acgcgccttc tgcgcgaggc
     1981 gggcgatcag ctcctcgatc ttcttgccca ccaccatcat caggtcggcg agctcgtcga
     2041 tcacgacgac gatgaagggc atgcgctcct gcggctcggg atcctcgggc gtcaggctga
     2101 acgggttggg gatcttctcg ccgcgctcgg cggcctcgtc aatcttcttg ttgtagccgg
     2161 cgaggttgcg cacgcccatc ttggacatga gcttgtagcg ccgctccatc tcgttgacgc
     2221 accacgacag cgcgttgccc gcctgcttca tgtcggtcac gaccgggcac agcaggtgcg
     2281 ggatgccctc gtagacgctc atctcgagca tcttcgggtc gatcaggatc agccggacgt
     2341 cgcgcccctc ggccttgtac agcagcgaca ggatcatcgc gttgatgccg accgacttgc
     2401 ccgagccggt ggtgccggcg accaggcagt gcggcatctt cgcgaggtcg gccacgaccg
     2461 ggttgccgac gatgtccttg cccagcccca tcgtcagcag cgagccggcc tcgttgtaga
     2521 cagtggagcc gaggatctcc gacagccgga tcgtctgccg gcgcgcgttg ggcagctcca
     2581 gcgccatcgt cgtcttgccg ggaatggtct cgacgacgcg gatgctcacg aggctcagcg
     2641 agcgggcgag gtccttggcg aggttgacga tctgcgagcc cttcacgccg gtggccggct
     2701 cgatctcgta gcgcgtgatg accggccccg gcgccgccgc gacgacgcgt acctcgacgc
     2761 cgaagtcgcc gagcttcttc tcgatcatgc ggctggtcat ctcgagcgac tcgggcgtga
     2821 ccgtctccac gcgcgacggc ggcacgtcga gcaggtcgac ctgcggcagc ctgttgtctg
     2881 cgatctcggc gaacagcggc ttctggcgct cgcgcgcgac gcgcttggac tcgggcacct
     2941 gcacgacctc gggctcgatc acgatgggca cgtgcgggac gtcgtcgacg cggacgtcgt
     3001 cggcggcgtg ctcgcgctcg cgcagggcga tctcgccgat gcgcaggtcc tcctcgcgct
     3061 ctcgctggac ggcgcggatc tcgcgccggc cgtcgatcca ggcgccgatc ccgtcggcca
     3121 gcgcgaccca cgagaagcgc agcgcccccg ccatgcccac gaccagcgcg gcgatccaga
     3181 acacgcccga gccgacgaag ccgagccagt gcatcgacgg cggcccgagc acgaagccca
     3241 gcacgccgcc cgagtggccg ccgggcacgc gaggctccca cgtgtacagg cgcgtccatt
     3301 cgagcgcggt gctcgcgcac atcagcagca cgagcccgcc ccagaaacgc aggcgcggcc
     3361 agcgcgcgac ggggccgtcc tcgcggccgg ccgacaacga gcgcgagctg cgcgccagcc
     3421 cgcgcagcgc ctgcagcagg cccacgaggg gaagccaagc caccgagccg ccgaacgccg
     3481 ccagcagcgc gtccgacagc caggcgcccg gccgcccgag caggttgagc accggcccgc
     3541 cgtcgcccga ggtggtgaag ccggggtcgg tcggccggtg cgaggcgagc gccattgcga
     3601 cggccgtcca ggcgaccagc tgcaacagcg ataccagccg ctgccgccag accggtcgcg
     3661 gcgcgaagtc gcgatcggaa gatccggcga acgccgggaa cttgggaaga gccaaactca
     3721 agaagcgcct aacgacatcg ggacgctgcg accggagcac ctccggcgcc gccgctcgca
     3781 tgtcccttgg aaagttcaag ggagggaggg atctggctcc gccagtcccg atccggagca
     3841 ccccctcggg gggcagcgac cagcgggagc gtgggggccg tcattcacga cttgcgattc
     3901 tggatcttca gggagccgtc ttcctgcgtc tcgatgatgc cccgttcctc gagatccttc
     3961 atcacgcggc tgaccatctc gcgcgaggcg cccacgatct tggccatgtc ctggcgcgag
     4021 accttggact tgatgacctt ctcgccgtcg atgtcctcgg ccatgtccag cagcgtgcgg
     4081 gcgacgcggc cgtagacgtc caggagggcg agcgactcga tctgccggtc ggcgctgcgc
     4141 aggcgcgtga ccaggccgcg catgatcgcg tacgacaggc tggagttctc gggcaggcag
     4201 cgcgcgaact cggcgcggcc gaggatcagc acgtcgcact gcagctcgca gcgcaccgtc
     4261 gcggagtgcg gctcgttgtc gatcaggctc atctcgccga cgtagtcccc cgcctgcagg
     4321 acggccagga tgacctcgcg gccgcgcgaa tcggacgtcg tgacgcgcgc acggccgctc
     4381 agcaggatga agagtgcgtt gctcttcttg ccctgctcga cgaccagttc gccgcgccgg
     4441 aagcggcgct tgacgacgcc gtccgccacc acccgcgcct gatcggtcgt gagcatggag
     4501 aacagcggga cgcgccggat caggtccaga ttcgagagca tcgacattca aaaacctttc
     4561 gcaaatccct ggggacgggg agcctgcatg ggggccgcca gcgggtcgcg aacggcatcc
     4621 ctacaatcgc ggggtgcatt gtgcctcgtg acattgtgcc gtcccaatcg aattttcccc
     4681 gagccgcccc ccgatgactt cccctgccaa cgactccaac cacgcccagg tgctgatcct
     4741 cggctccggc ccggccggct acacggcggc gctgtacgcc gcccgtgcga acctcaggcc
     4801 gatgctggtc accggccttg cccagggcgg ccagctcatg acgaccaccg aggtggacaa
     4861 ctggcccgcc gacgtcgcgg gcgtccaggg ccccgacctg atggcgcgct tcctggcgca
     4921 cgccgaacgc ttcgacacga agatcgtctt cgaccacatc aacaaggtcg acctctcgag
     4981 gcgcccgttc accctggtcg gcgactcggg cacctacacc tgcgacgcgc tggtcatcgc
     5041 cacgggcgcc tcggccaagt acctcgggct gccgtccgag gagtccttca tgggccgcgg
     5101 cgtcagcggg tgcgcgacct gcgacgggtt cttctaccgc ggcgaggaag tctgcgtcgt
     5161 gggcggcggc aacaccgccg tcgaggaggc gctgtacctg gccaacatcg ccagcaaggt
     5221 gcacctgatc caccgccgca acaagttccg cgccgagccc atcatgatcg acaagctgat
     5281 gcagaaggcg gccgagggca ggatccagct gcacctcgac cagacgctgc acgaggtgct
     5341 gggcgaccag accggtgtca ccggcgtgcg cacgaagtcc gtcgagggcg aggcctacgc
     5401 cgagatcgcg gtcaagggct gcttcatcgc gatcggccac tcgcccaaca cgtcgatctt
     5461 cgagggccag ctgaagatga aggacggcta catcgtcacg cgcagtggca acgagggctt
     5521 cgcgacgatg accagcgtcg agggcgtctt cgcggcgggc gacgtgcagg atcacgtcta
     5581 tcgccaggcc atcacctcgg ccggcaccgg ctgcatggcc gcgctggacg cgcagcgctt
     5641 cctcgagcag ttcgcctgac cggtcgccgg ccgttcgtgg ccgggtcggg cgggcgccac
     5701 cggcacgccc tggccgcggc ggccgaattc gtgtatgatc cgcgattccc ctggagtcgt
     5761 gccgccctct tggcgtaagt gcgcggcctc cacccggatc gcatggggcg ttccgcgcaa
     5821 tcaaggagag cttcatggct cgcgtgtgtc aagtcacggg caagcgcccg atggtcggga
     5881 acaacgtttc ccacgccaac aacaagacga agcgtcgctt cctgccgaac ctgcagtacc
     5941 gccgcttctg ggtcgagagc ga
//
LOCUS       DASVLN010000002         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_376285, whole genome shotgun sequence.
ACCESSION   DASVLN010000002 DASVLN010000000
VERSION     DASVLN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376285"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VF453_00035"
     CDS             complement(<1..669)
                     /locus_tag="VF453_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX7686054.1"
                     /translation="MCSNYLPVASRDRMLAAFGVALDRDQDDREVFPLGSAPFIRLAV
                     EGEEGGRPALVAETGQFGLLPPWAAEHRFGTRTYNARSETVARLPSFRDAWAQGHRCI
                     VPVEAVFEPNWESGDCVRWRIARESGEPLGVAGLYTQWKSPEGERRFTFTMLTVNADA
                     HAFYARFHRPGEEKRMPAFLDPHEYGPWLACKPFQAPRFLRMWPGPLSGEPAPLARAT
                     RAAKP"
     gene            complement(748..>2068)
                     /locus_tag="VF453_00040"
     CDS             complement(748..>2068)
                     /locus_tag="VF453_00040"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEX7686055.1"
                     /translation="TQRGTVVPVDRRGRTPLPALIWLDQRRARQVPRINPMWRAAFRL
                     AGVAETVRYFQREAEVNWWAENEPKQWGRVHKLLLLSGYLNWKLTGRFADSIGSQVAY
                     VPFDYKAHAWAKSWDWKWQALSVTPEQMPELLPVGTVIGKIGNAASTATGIPLGVPVV
                     AAAADKACEVLGSGCLAPHVGGVSYGTTATINVCTTQYAEALAFVPPYPAALPGHYAS
                     EVQIFRGYWMVRWFKEQFGDREVLAAAERGIAPEILLDELVAAVPPGSLGLTVQPYWT
                     PGIRHPGPDAKGAIIGFGDAHTRAHVYRAILEGLAYALRDGRERIERKTRVAVTELRV
                     SGGGSQSDAAMQLTADVFRLPASRPHTYETSGLGAAIDCAVGLGLHADFPSAVKAMTR
                     VARTFEPDPRAADLYDQLYAQVYSRMYARLAPLYARIREITGYPARR"
BASE COUNT          281 a          744 c          772 g          271 t
ORIGIN      
        1 cggcttcgcg gcgcgcgtgg cccgcgcgag cggcgcgggc tcgccgctca gcggccccgg
       61 ccacatccgc aggaagcgcg gcgcctggaa aggcttgcag gcgagccacg ggccgtactc
      121 gtgcggatcg aggaaggccg gcatgcgctt ctcctcgccc gggcggtgga agcgggcgta
      181 gaacgcatgg gcgtcggcgt tgaccgtcag catcgtgaac gtgaagcggc gttcgccctc
      241 ggggctcttc cattgcgtgt acaggccggc cacgcccagc ggctcgccgc tttcccgcgc
      301 gatgcgccag cgcacgcagt cgcccgactc ccagttcggc tcgaacaccg cctccaccgg
      361 cacgatgcag cggtggcctt gcgcccaggc gtcgcggaag ctgggcaggc gcgcgaccgt
      421 ctcgctgcgc gcgttgtagg tgcgggtgcc gaagcggtgc tcggccgccc acggcggcag
      481 gagcccgaac tggccggtct cggcgaccag ggcgggccgt ccgccctcct cgccctcgac
      541 cgccaggcgg atgaacggcg ccgacccgag cgggaagacc tcgcggtcgt cctggtcgcg
      601 gtcgagggcg acgccgaagg cggccagcat ccggtcgcgg ctggcgacgg gcaggtagtt
      661 cgagcacatg ggccggatga tacggagacg caccgcttgc agccgaaggc ccggcaggct
      721 cgccggagga cgccgggacg ccgcggctca gcgccgcgcc ggatagcccg tgatctcgcg
      781 gatccgcgcg tacagcgggg cgagccgcgc gtacatccgc gagtagacct gcgcgtagag
      841 ctggtcgtag aggtcggcgg cgcgaggatc gggctcgaag gtgcgggcga cgcgcgtcat
      901 cgccttcacg gccgacggga agtcggcgtg caggcccagg ccgacggcgc agtcgatcgc
      961 ggcgcccagc ccgctcgtct cgtaggtgtg agggcggctg gcgggcaggc ggaagacgtc
     1021 ggccgtcaat tgcatcgccg cgtccgactg cgagccgcca cccgagacgc gcagctccgt
     1081 gaccgcgacc ctggtcttgc gctcgatgcg ctcgcggccg tcgcgcagcg cataggccag
     1141 gccttcgagg atcgcgcggt agacgtgggc gcgggtgtgg gcgtcgccga agccgatgat
     1201 cgcccccttc gcgtccgggc cgggatggcg gatgcccggc gtccagtagg gctgcacggt
     1261 gaggcccagc gagcccggcg gcacggcggc gacgagttcg tcgagcagga tctccggcgc
     1321 gatcccgcgc tcggccgcgg caagcacctc gcggtcgccg aactgctcct tgaaccagcg
     1381 caccatccag tagccgcgga agatctgcac ctcgctcgcg tagtgtcccg gcagcgccgc
     1441 gggatacggc ggcacgaagg cgagcgcctc ggcgtactgc gtcgtgcaca cgttgatggt
     1501 ggccgtggtg ccgtaggaga cgccgccgac gtgcggcgcc aggcagcccg agcccagcac
     1561 ctcgcacgcc ttgtcggccg ccgccgcgac caccggcacg cccagcggga tgcccgtggc
     1621 cgtgctcgcg gcgttgccga tcttgccgat cacggtgccg accggcagca gctcgggcat
     1681 ctgctcgggc gtcaccgaga gcgcctgcca tttccagtcc caggacttcg cccacgcgtg
     1741 ggccttgtag tcgaacggca cgtaggcgac ctgcgagccg atcgagtcgg cgaagcggcc
     1801 ggtgagcttc cagttcaggt accccgacag cagcagcagc ttgtgcacgc ggccccactg
     1861 cttcggctcg ttctccgccc accagttgac ctcggcctcg cgctggaagt agcgcaccgt
     1921 ctcggccacg ccggcgagcc ggaacgccgc gcgccacatc gggttgatgc gcggcacctg
     1981 gcgcgcgcgc cgctggtcga gccagatcag cgccggcagc ggcgtgcggc cgcgccggtc
     2041 gaccggcacg acggtgccgc gctgggtc
//
LOCUS       DASVLN010000003         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_319, whole genome shotgun sequence.
ACCESSION   DASVLN010000003 DASVLN010000000
VERSION     DASVLN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VF453_00045"
     CDS             <1..286
                     /locus_tag="VF453_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686056.1"
                     /translation="GSRLGLVHGVRRIEARDALPPALRSGLSGVDGVLLASAAAGTIA
                     WAALVPAGDALAPASTATGIEAFILQMALVGWRIWRSAPPPLSPPPATQP"
     gene            complement(390..944)
                     /locus_tag="VF453_00050"
     CDS             complement(390..944)
                     /locus_tag="VF453_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX7686057.1"
                     /translation="MPTPFADPRVVLSFWFEEIEPSQWWGKSPAFDRVVAERFGALHR
                     AASRCELSGWRATPEGRLAEVIVLDQFPRNMFRDDGRAFATDALALALAQTAVALGAD
                     RALDPQRRVFLYMPYMHSESPVVHETALVLFETLGIADNLEYERRHKAIVDRFGRYPH
                     RNAMLGRESTHEELEFLKTPGSGF"
     gene            1089..1361
                     /locus_tag="VF453_00055"
     CDS             1089..1361
                     /locus_tag="VF453_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686058.1"
                     /translation="MKLKTVLLVTAFAGASAAVVAAPPENVSARRHPNIAAAQRLVDQ
                     AYRKVVAAQQANEYDMDGHAAKAKELLEQADRELKAAAEAANANRK"
     gene            1524..1796
                     /locus_tag="VF453_00060"
     CDS             1524..1796
                     /locus_tag="VF453_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEX7686059.1"
                     /translation="MDKQLFIRTALASVIALGAVSAAHAGPAAPQPGKDKCFGVAKAG
                     QNDCASATGSHSCAGTATKDNDPGDWKYVDKGTCEKMGGHLAMAKK"
     gene            1831..>2671
                     /locus_tag="VF453_00065"
     CDS             1831..>2671
                     /locus_tag="VF453_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX7686060.1"
                     /translation="MTFSTLPARAAGIGLRQPHYREMFERAPAIGFVEVHSENFFADG
                     GAALHALERARAAWPVSLHGVGLSIGSADALAEAHLGKLARLVERIEPAVVSEHLCWC
                     GVGGTSLNDLLPLPYTQAALAHVAARVERVQERLRRPILLENLSAYVAFRGSEMTETA
                     FLSELARRTGCGLLLDVNNLHVNAVNFDFDAQARLAELDGAKIGEIHLAGHAVVDDCL
                     VDTHGGTVAEPVWALYEAACRRFGPKPTLVEWDTDLPALDVLLGEAASAARMMAGTAE
                     AAHA"
BASE COUNT          386 a          986 c          956 g          343 t
ORIGIN      
        1 cggcagccgg ctgggcctcg tccatggcgt ccgccggatc gaggccaggg acgccctgcc
       61 gcccgcgctg cggtcggggc tctcgggggt ggacggcgtg ttgctcgcct cggccgcggc
      121 ggggacgatc gcctgggcgg cgctggtgcc cgcgggcgac gccctggcgc cggcctcgac
      181 ggcgaccggc atcgaggcgt tcatcctgca gatggcactt gtcggctggc ggatctggcg
      241 ctcggcaccg ccccccctct ccccgccgcc cgccacgcag ccgtgaccgc aagcgcgcac
      301 gatcggcacc gccagcacgc ccacgcccgc cgacgcctgc ccgttcgccc cggcgctggc
      361 cgatgtgccc ggggggtcgc tgactcggct cagaagcccg acccgggcgt cttcaggaac
      421 tcgagttcct cgtgcgtcga ctcgcgcccg agcatcgcgt tgcggtgcgg atagcggccg
      481 aagcggtcga cgatcgcctt gtggcggcgt tcgtactcga ggttgtcggc gatgcccagc
      541 gtctcgaaga ggaccagcgc cgtctcgtgg acgacaggcg actcgctgtg catgtagggc
      601 atgtagagga agacgcgccg ctgcggatcg agcgcgcggt cggctccgag cgcgacggcc
      661 gtctgggcga gcgccagcgc gagcgcgtcg gtcgcgaacg cgcgcccgtc gtcgcggaac
      721 atgttgcgcg ggaactggtc gaggacgatg acctccgcga gccggccttc cggcgtcgcg
      781 cgccagccgg agagctcgca ccgcgacgcc gcccgatgca gggcgccgaa gcgctcggcg
      841 acgacgcgat cgaaggcggg cgacttgccc caccactggc tcggctcgat ctcctcgaac
      901 cagaacgaca ggacgacgcg ggggtcggcg aacggcgtgg gcatgcggcg atcatacggc
      961 cgtcagctcg cgcacagctt cgggcgcgcc ctgtcaacac gcgctcggcg tcggcgttga
     1021 gacgggacag gcgagcgccg ccgcgtgcga tcgaccgcct gatccctcca tccaccggaa
     1081 agacccgcat gaagctcaag accgtcctcc tcgtcaccgc cttcgccggc gcctccgccg
     1141 cggtcgtcgc cgcgccgccg gaaaacgtca gcgcgcgcag gcatcccaac atcgccgcgg
     1201 cgcagcgcct cgtcgaccag gcctaccgga aggtggtcgc ggcgcagcag gccaacgaat
     1261 acgacatgga cggccacgcc gccaaggcca aggagctcct ggagcaggcg gacagggaac
     1321 tgaaggcggc ggccgaggcc gccaacgcca accggaagtg acggcgtgca cccgggcggc
     1381 gggacgcctc cgcgggtgcc gcgtgcgcga tgatggcgag cggggtcgcg aaatttttcg
     1441 cgggggcatg taagaaagcg ccggggccgt cgacagacgt tcgtgccgct cgaggcgcac
     1501 tcttcaccaa ccaaggaatc cccatggaca agcaactctt catccgtacc gccctcgcct
     1561 ccgtcatcgc cctgggcgcg gtcagcgccg cccacgcggg ccccgccgcg ccgcagcccg
     1621 gcaaggacaa gtgcttcggc gtcgccaagg ccggccagaa cgactgcgcc agcgcgaccg
     1681 gctcgcactc gtgcgccggc acggccacca aggacaacga cccgggcgac tggaagtacg
     1741 tcgacaaggg cacctgcgag aagatgggcg gccacctggc gatggccaag aagtgacggc
     1801 cgcggccacg acccctcgcc cgcccacgcg atgacgttct ccacgcttcc cgcccgcgcg
     1861 gccggcatcg gcctgcggca gccgcactac cgcgagatgt tcgagcgcgc ccccgcgatc
     1921 ggcttcgtgg aggtgcacag cgagaacttc ttcgccgacg gcggcgccgc cctgcacgcg
     1981 ctggagcgcg cgcgggcggc gtggcccgtg agcctgcatg gggtcgggct gtcgatcggc
     2041 tcggccgacg cgctcgccga ggcacatctc ggcaagctgg cgcgcctggt cgagcgcatc
     2101 gagccggccg tcgtctccga gcacctctgc tggtgcggcg tcggcggcac ctcgctgaac
     2161 gacctgctcc cgctgccgta cacgcaggcc gcgctggccc acgtcgcggc acgcgtcgag
     2221 cgcgtgcagg agcggctgcg ccggcccatc ctgctcgaga atctctcggc ctacgtcgcc
     2281 ttccgtggca gcgagatgac cgagacggcc ttcctctcgg agctcgcgcg ccgcaccggg
     2341 tgcgggctgc tgctggacgt gaacaacctg cacgtcaacg ccgtgaactt cgacttcgac
     2401 gcgcaggcac ggcttgccga gctcgacggt gcgaagatcg gcgagatcca cctggcgggc
     2461 catgccgtcg tcgacgactg cctggtcgat acccacgggg gtaccgtcgc cgagccggtg
     2521 tgggcgctgt acgaggccgc gtgccggcgc ttcgggccga agccgacgct cgtcgagtgg
     2581 gacaccgacc tgcccgcgct cgacgtgctg ctgggcgagg ccgcgtcggc cgcgcgcatg
     2641 atggccggca ccgcggaggc cgcgcatgcg t
//
LOCUS       DASVLN010000004         9845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_107807, whole genome shotgun sequence.
ACCESSION   DASVLN010000004 DASVLN010000000
VERSION     DASVLN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9845
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107807"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF453_00070"
     CDS             complement(<1..190)
                     /locus_tag="VF453_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686061.1"
                     /translation="MTPDDAAESAARDLAPPWIEGTLTQTREDLVAESLALVALLFSV
                     TIGIVIALLPVSGAEARDV"
     gene            complement(373..1005)
                     /locus_tag="VF453_00075"
     CDS             complement(373..1005)
                     /locus_tag="VF453_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7686062.1"
                     /translation="MTKIYLIDDHPIVRAGLRALLEGAGHAIVGEGSDITQALSEVNR
                     MQPEIVLLDLNLDGRSGFEVLAELHRRNQSSRAIVLSMSDQPRNVAEALRLGASGYVL
                     KGSASTELLSAVQAVAAGRRYLGAGLTDIAVQGLTSDADKGSTLSPRERQILVMVARG
                     KSSAAIGEELHLSPKTVDTYRSRLMAKLELADIPALVRWALREKLIDLDD"
     gene            complement(1287..1670)
                     /locus_tag="VF453_00080"
     CDS             complement(1287..1670)
                     /locus_tag="VF453_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7686063.1"
                     /translation="MSSADRPAEAAQRVMLVDDNVDALESLEMLLQMLGFEVATAAHP
                     EAALELAERFDPSIALIDIGLPGMDGYQLAAEMRRRRAGRPLRLVAFTGYGGAEDVAR
                     AHAAGFDAHLVKPVDMDQLVALLGA"
     gene            1826..3160
                     /locus_tag="VF453_00085"
     CDS             1826..3160
                     /locus_tag="VF453_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7686064.1"
                     /translation="MHPGDDHAHDEQGGATPRPDPTREERAMNRREDVHVIPAADEAA
                     VLMLEDVDAEGAPRSRRRERVVLHTPVDVRSGSAVVLSVLLSLYALHWAAEIVIPVLM
                     GLLFSYALSPVVDRLCRLRVPRPLSAGIVMVMLVGGLAWATYSLSDQANNLLESLPDA
                     AQKLRSTLHANARHNDNTTMAQVQKAAATLELAAADAGSRPAPGVAKVQIVKPGFNIQ
                     DYLLTGTVRAAEGALQAIVVAFITYFLLAAGDTFRRKLARIAGPSFAKRKITVQALDE
                     ISDQVRRYLLVQLLGSVIVGAATGLAFLALGMNNAAVWGVVAGVLNLIPYVGTAVTGG
                     ASAVVALLQFGTIKMALAVAAVSVVAHIVTGWGLTPWLTSRTSRLNAVVVFIGVLAWG
                     WLWGVWGLLLGTPILMAVKAVCDRVDDWKPLGELMGGNDRPGDRVASGTAEP"
     gene            complement(3181..3657)
                     /locus_tag="VF453_00090"
     CDS             complement(3181..3657)
                     /locus_tag="VF453_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7686065.1"
                     /translation="MAVVHAPRQPAAHAPAPAPAHEAVAPAAPPISLLLVEPQFLLRR
                     TVAAVARDLRLAEPHETTTIDAAARLVAGRSFEALFLSLDEEDLALELMARVRGGETK
                     CAATTPIAVTAGACDTSLALRLKQLEVRRLLLRPFKVKGVLDAIAALRLAPVPAAS"
     gene            complement(3657..4154)
                     /locus_tag="VF453_00095"
     CDS             complement(3657..4154)
                     /locus_tag="VF453_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7686066.1"
                     /translation="MLAAPIPTDDRERLQALRALLILDTPPEERFDRLVRFAADEFEM
                     PMVFVSLVDEKRQWFKARVGLDTCATDRESSFCGHAILKPEFFVVEDTLADERFADNP
                     LVTGEPHLRFYAGAPLQLPGGERVGTLCLADRRPRALDAVDLAILGSLRDLVVEELVQ
                     RTEAR"
     gene            complement(4255..7290)
                     /locus_tag="VF453_00100"
     CDS             complement(4255..7290)
                     /locus_tag="VF453_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7686067.1"
                     /translation="MAGRVDPNEAHTRRRTWLRLTLAALALSLAGLVGVTARFATEAE
                     RTLQQVSRQAALSLRLRALQDVTVALLDAETGQRGYLLTNDERYLGPYRAAVARMPAL
                     LASLDGRAEVADPGVARKSQEMRQLVTDKLAEMDETVRLQAGGRHDRALALMLSDRGR
                     DLMLRTRADVGELLDAARDARDRIGVDIAAAAARVQRLLLLAVGSLLVFVALAIGQLV
                     QALAVRRRIAHDLALSEQRHRALVEDQSELVSLARRDGTLVYVNPAFARHFGHAPAAM
                     VGMDRHELMAAEADRDAMRLEVADVLRSGRARRSENRIVDADGHERWISWTNQRREEP
                     DGVLLHSVGRDITERKRAERALRASQAFLYRTGRIAGVGGWEKDLVGGDDVWSEEVRR
                     IMEADDDYAPTREQIVASYAPEARATMDRAISDCIERGVPWDLELPRITCKGRRIWVR
                     TVAGLEFENGRPRRIVGALQDITERKQLEQRLADSEHFLRQITDSLPVRIAYLDTRAR
                     YRFVNWAHCKRFGLEREEIVGRTRSEVNRGQADDVVGPRIAAVLRGEPQQFEYDDAVG
                     GHVRRIDCSLLPDVGDDGRVRGFYATGIDITDRVSSERRLRELTQILELTPDFVVQAN
                     RRGVLEYVNPALRHALGIPVDAPLGRWTIADFVTPQTAERYAREIQPVLRTRGCWIGE
                     ATLQLAAGVTPVNHLVIAHRGADGRVEHLSAVMRDISGEVAARDALALQTAALESVVE
                     TLPAMVAVVGRDGRYRLVNSAFERAVQLPRERILGRAIHELVTPDEYARRRPWIERAL
                     RGETVSFETYDAARRFRHLSLTYTPLRIGAQPDGFVGMALDVTQQKDEAGRLLALAQT
                     DAQTGLLNRAGLQAWLEERVRADGIAQVALLYIDLDHFKPVNDTFGHSAGDDVLRLFA
                     LRLRELVRPADAVARLGGDEFALALTGLREREHALAVAAKVVEAAGRPFAVGALDVSI
                     GASVGVAFQVESVEDAPGLIAHADAMLYAAKAAGRNTFA"
     gene            complement(7393..8193)
                     /locus_tag="VF453_00105"
     CDS             complement(7393..8193)
                     /locus_tag="VF453_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686068.1"
                     /translation="MTHARSSRVSRALPASTALPFLRALAIAGLAAASAVAPAARASA
                     PAETHALAISWEPAFCLTKSGKPECTSETPSRADAHQFSLHGLWPQNGSYCGVSAAQK
                     SEDQAGEWSMLPAVTLQPATESLLQVDEPGTMSYLERHEWTEHGTCSGLSQQNFFAPT
                     LGMLGSVNASNLGATVAANVGGHVTLSQLQSAAALDFGTYAKSDIEYLCVSSGGKSWL
                     EEVRFHLHLPTPLPSSVQASYLTLPKQPASSSQLCKASASIYIRAVAN"
     gene            8453..9247
                     /locus_tag="VF453_00110"
     CDS             8453..9247
                     /locus_tag="VF453_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX7686069.1"
                     /translation="MPDDTLVSADLRLAGRLDARFFALLEAIAATGSINRAARTAGLS
                     YRGAWLILDNAANLARSPLVESAIGGSGGGGTRLTDMARELMAAWRDLQSRHHAFLRE
                     QEAWLLQRPALAGALRRMAMKTSARNQFAGTIVDLARGPVSTEATLELAGGQRITAAV
                     TTQAAERMGLAAGQEAIALIKASAIVLVADFAGYVLSARNQLAGTVSRIERGAVSSLV
                     ALTLPGGTVVTASVTNDAVDALDLAVGHAASAVFKAYSVLLAVAAK"
     gene            complement(9251..9733)
                     /locus_tag="VF453_00115"
     CDS             complement(9251..9733)
                     /locus_tag="VF453_00115"
                     /inference="COORDINATES: protein motif:HMM:NF020947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HEX7686070.1"
                     /translation="MSAAPFPEAGVDAGREPQGSSTGGDDLRIAIVVDPSLPAGLVAN
                     TVAAIGIGLGAAATVLGGDVLVDADGRAVRTSSNRPVPILQATPAAMAALLARAQVVP
                     EGGVVAPFPRFARSLHRFEDYLAEFPRRRLAGEAIDGLGIAGPGKWVRSLTGSLKLLR
                     "
BASE COUNT         1286 a         3513 c         3678 g         1368 t
ORIGIN      
        1 cgacgtcccg ggcctcggcg cccgagaccg gcagcagcgc gatcacgatg ccgatcgtca
       61 ccgagaacag cagggcaacc agcgcgaggc tctcggcgac gaggtcctcg cgggtctgcg
      121 tcagcgtgcc ttcgatccac ggcggggcca ggtcgcgggc ggcggattcg gcggcgtcgt
      181 ctggcgtcat ggggtcctcg tccctggctg gtcgtccggc ggcgcgccgg gggcgcaccg
      241 cgcgcgggcg gtcgagcgcc tcgcgtccct ggtcttcgga cagtctggcc tgcggccgag
      301 gacgccgctg tgggggcatc tggacgcgtc gcgtcaggca accgaagcgc gcgccgccct
      361 gccggcgggc attcagtcgt cgaggtcgat caacttctcg cgcagcgccc agcgcacgag
      421 cgccggaatg tccgcgagct cgagcttggc catcaggcgg ctgcggtacg tgtccaccgt
      481 cttgggcgac agatggagct cctcgccgat cgccgcgctc gacttgccgc gcgcgaccat
      541 cacgaggatc tggcgctccc gcggcgacag cgtgctgccc ttgtcggcat cgctggtcag
      601 gccctggacg gcgatgtcgg tcagcccggc gccgaggtag cgccggccgg cggccacggc
      661 ctggacggcg gacagcagct cggtgctcgc ggagcccttg agcacgtagc cggaggcgcc
      721 caggcgcagc gcctcggcga cgttgcgcgg ctggtcggac atcgacagca cgatcgcgcg
      781 cgaggactgg ttgcggcgat gcaactcggc gagcacctcg aagcccgagc ggccgtcgag
      841 gttcagatcc agcagcacga tctccggctg catcctgttg acctccgaca gcgcctgcgt
      901 aatgtcggag ccttcgccga cgatggcgtg gcccgcgcct tcgagcagcg cgcgcagccc
      961 ggcgcggacg atcggatggt cgtcgatgag atagatcttg gtcattccct ggtctcccga
     1021 tcctccctgc cgatcccgcg gcggggacga tgttcccgtt cgctcgcgcc gcgcatgcgt
     1081 gcccggcgcg agcctccctt gccgatcgcc gcctctctcg cgcgatgcca gggcgacgcg
     1141 cgggacggat tcagggtcct cgaagcacgt tacgccgcgc ttcacccgcg gtcaatggcg
     1201 aaatcgacag ttgagactgt gccgcgcgct gcagtcgcat gtgcgcgcaa cagcgcgcgt
     1261 aaccggttcc aattccgcgg gcgagttcag gcgcccagga gggcgacgag ctggtccatg
     1321 tccaccggct tgaccaggtg cgcgtcgaag cccgcggcgt gcgcgcgggc gacgtcttcg
     1381 gcgccgccgt agcccgtgaa cgccacgagg cgcagcgggc ggccggcgcg gcggcgccgc
     1441 atctccgcgg cgagctggta gccgtccatg cccggcaggc cgatgtcgat cagcgcgatc
     1501 gacggatcga agcgttcggc gagctcgagc gccgcctcgg ggtgcgccgc ggtcgcgacc
     1561 tcgaagccga gcatctgcag cagcatctcc agcgattcga gcgcgtcgac gttgtcgtcg
     1621 accagcatca cgcgctgggc ggcttcggcg gggcggtcgg cggaggacat ggcggggggg
     1681 cggcggacgc ggaagttgcg cggattatgg cagccgcgcg cgtgcctgcg cacgtcgcgg
     1741 cgccgctgtc ctacacgacg caatccgctg cgcctaccgc cggcggcggc caccccgtcg
     1801 acactggccg ggagactcga caacgatgca cccaggagac gaccatgccc acgacgagca
     1861 gggcggcgcc acgccgcgcc cggacccgac gcgcgaggag cgcgcgatga accggcgcga
     1921 ggacgtgcac gtgatcccgg cggccgacga ggccgccgtc ctcatgctgg aggacgtcga
     1981 tgccgagggc gcgccccgct cgcgccgccg cgagcgcgtc gtcctgcaca cgccggtgga
     2041 cgtgcgcagc ggctcggcgg tggtgctgtc ggtgctgctc agcctgtacg cgctgcactg
     2101 ggcggcggag atcgtcatcc ccgtgctgat ggggctcctg ttcagctacg cgctgtcgcc
     2161 ggtcgtcgac cgcctgtgcc gcctgcgcgt gccgcgcccg ctgtccgcgg gcatcgtgat
     2221 ggtcatgctg gtgggcgggc tggcgtgggc gacctactcg ctcagcgacc aggccaacaa
     2281 tctgctggag tcgctgcccg acgccgccca gaagctgcgc tcgacgcttc acgccaacgc
     2341 gcgccacaac gacaacacca ccatggcgca ggtgcagaag gccgcggcga cgctcgagct
     2401 cgcggccgcg gacgccggct cgcgcccggc gccgggcgtc gccaaggtgc agatcgtcaa
     2461 gccgggcttc aacatccagg actacctgtt gaccggcacc gtgcgcgccg cggagggcgc
     2521 gctgcaggcg atcgtcgtcg cgttcatcac ctacttcctg ctcgccgcgg gcgacacctt
     2581 ccggcgcaag ctcgcgcgca tcgcgggacc ctccttcgcc aagcgcaaga tcacggtgca
     2641 ggcgctcgac gagatcagcg accaggtacg ccgctacctg ctcgtgcagc tgctcggcag
     2701 cgtgatcgtc ggcgccgcca ccgggctcgc gttcctcgcg ctgggcatga acaacgccgc
     2761 ggtctggggc gtcgtggccg gcgtgctgaa cctcatcccg tatgtcggca ccgccgtcac
     2821 gggcggcgcc tcggccgtcg tcgcgctgct gcagttcggc acgatcaaga tggcgctggc
     2881 ggtggcagcg gtctcggtcg tcgcccacat cgtcaccggc tggggcctca cgccctggct
     2941 cacgagccgc acgagccggc tcaacgcggt ggtcgtcttc atcggcgtgc tcgcctgggg
     3001 ctggctgtgg ggcgtctggg gcctgctgct gggcacgccc atcctgatgg ccgtcaaggc
     3061 cgtctgcgat cgcgtcgacg actggaagcc gctcggcgag ctgatggggg gcaacgaccg
     3121 gcccggtgac cgcgtcgcct ccgggaccgc ggaaccctag gtgcccggct gccggtgcct
     3181 tcaggacgcg gccggcacgg gcgcgaggcg cagtgcggcg atcgcgtcga gcacgccctt
     3241 gaccttgaac ggccgcagca gcaggcgccg cacctccagc tgcttgagcc gcagggcgag
     3301 cgaggtgtcg caggcgccgg ccgtgacggc gatcggcgtc gtcgcggcgc atttcgtttc
     3361 gccgccgcgc acgcgcgcca tcagctcgag cgccaggtct tcctcgtcga gcgacaggaa
     3421 cagcgcctcg aacgatcgtc ccgccacgag ccgcgcggcg gcgtcgatgg tggtggtctc
     3481 gtgcggctcg gccaggcgca ggtcgcgcgc gaccgccgcg acggtgcggc gcaggaggaa
     3541 ctgcggctcg accagcagca gcgagatcgg cggcgccgcc ggcgccaccg cctcgtgcgc
     3601 tggcgctggc gctggcgcgt gcgctgccgg ctgccgcggc gcgtggacga cggccatcag
     3661 cgggcctccg tgcgctgcac gagttcctcg acgacgaggt cgcgcaacga gcccaggatc
     3721 gcgaggtcga cggcatcgag cgcgcgcggc cggcggtcgg ccaggcacag cgtcccgacc
     3781 cgttcgccgc ccggcagctg caggggcgcg ccggcgtaga agcgcaggtg cggctcgccg
     3841 gtgacgagcg ggttgtcggc gaagcgctcg tcggcaagcg tgtcctcgac gacgaagaac
     3901 tccggcttca ggatcgcgtg accgcagaac gagctctcgc ggtcggtcgc gcaggtgtcg
     3961 aggccgactc gggccttgaa ccactggcgc ttctcgtcga ccaggctcac gaagaccatc
     4021 ggcatctcga actcgtcggc ggcgaagcgg acgaggcggt cgaagcgctc ctcgggcggg
     4081 gtgtcgagga tcagcagcgc ccgcagcgcc tgcaggcgtt cgcggtcgtc ggtcgggatg
     4141 ggggcggcaa gcatggcggc gtcggcgcgg cgggtcgggg acttcgtggt cttcggccgc
     4201 cgggagcgga acttgagcgc gagtcggata ccccggcccg tgtctgccgc gcagctaggc
     4261 gaaggtgttg cgcccggccg ccttggccgc gtagagcatc gcgtcggcgt gcgcgatgag
     4321 gcccggcgcg tcctccaccg attcgacctg gaacgccacg cccacgctcg cgccgatcga
     4381 gacgtccagc gcaccgacgg cgaacggccg ccccgcggcc tcgaccacct tggcggccac
     4441 cgccagcgcg tgctcgcgct cgcgcagccc ggtcagcgcg agcgcgaact cgtcgccgcc
     4501 cagccgcgcc accgcgtcgg cgggccgcac gagctcgcgc aggcgcagcg cgaacaggcg
     4561 cagcacgtcg tcgccggcgg aatggccgaa ggtgtcgttg accggcttga agtggtcgag
     4621 gtcgatgtac agcagcgcca cctgcgcgat gccgtcggcg cggacgcgct cctccagcca
     4681 ggcctggagg ccggcgcggt tgaggaggcc cgtctgggcg tccgtctgcg cgagcgcgag
     4741 caggcgcccg gcctcgtcct tctgctgcgt gacgtcgagc gccatgccga cgaagccgtc
     4801 gggctgggcg ccgatgcgca gcggcgtgta ggtcaggctc aggtggcgga agcgccgcgc
     4861 cgcgtcgtaa gtctcgaagc tgaccgtctc gccgcgcaat gcgcgctcga tccagggccg
     4921 gcgccgcgcg tactcgtcgg gcgtgacgag ctcgtggatc gcgcgcccga ggatgcgctc
     4981 gcgcggcagc tggacggccc gttcgaacgc gctgttcacg agccggtagc ggccgtcgcg
     5041 gccgacgacg gccaccatcg cgggcagcgt ctcgaccacc gactccagcg cggccgtctg
     5101 cagcgccagc gcgtcgcgcg ccgcgacttc accggagatg tcgcgcatca cggccgacag
     5161 gtgctcgacg cgcccgtccg cgccgcggtg ggcgatcacg aggtggttga ccggggtcac
     5221 gcccgcggcc agctgcagcg tcgcctcgcc gatccagcac ccgcgcgtgc gcagcacggg
     5281 ctggatctcc cgggcgtagc gctcggcggt ctgcggggtg acgaagtcgg cgatcgtcca
     5341 gcggccaagc ggcgcatcca ccgggatgcc cagggcatgc cgcagcgccg ggttgacgta
     5401 ctcgaggacg ccgcggcggt tcgcctgcac gacgaagtcg ggcgtcagct cgaggatctg
     5461 cgtcagctcg cgcagccggc gctcgctcga gacacgatcc gtgatgtcga tgccggtcgc
     5521 gtagaagccg cggacgcgac cgtcgtcgcc gacgtcggga agcaggctgc agtcgatgcg
     5581 gcgcacgtgg cctccgaccg cgtcgtcgta ctcgaactgc tgcggctcgc cgcgcagcac
     5641 ggcggcgatc cgcgggccga cgacgtcgtc cgcctggccg cgattgacct ccgagcgcgt
     5701 gcggccgacg atctcctcgc gctccagccc gaagcgcttg cagtgcgccc agttgacgaa
     5761 ccggtaccgg gcacgggtat cgaggtaggc gatgcgcacg ggcaggctgt cggtgatctg
     5821 gcgcaggaaa tgctcgctgt cggccaggcg ctgctcgagc tgcttgcgct cggtgatgtc
     5881 ctgcagcgcg ccgacgatgc gacgcgggcg gccgttctcg aactcgaggc cggccacggt
     5941 gcgcacccag atgcgccggc ccttgcaggt gatgcgcggc agctccaggt cccacggcac
     6001 cccgcgctcg atgcagtcgg agatcgccct gtccatggtc gcgcgggcct cgggggcgta
     6061 gctcgcgacg atctgctcgc gcgtgggcgc gtagtcgtcg tcggcctcca tgatgcggcg
     6121 cacttcctcg gaccagacgt cgtcgccgcc gacgaggtcc ttctcccagc cgccgacgcc
     6181 ggcgatgcgg ccggtgcggt agaggaaggc ctggctcgcg cgcagcgcgc gctcggcgcg
     6241 cttgcgctcg gtgatgtcgc ggccgacgga gtggagcagc acgccgtcgg gctcctcccg
     6301 gcgctggttc gtccacgaga tccagcgctc gtggccgtcg gcgtccacga tgcggttctc
     6361 gctgcgccgc gctcgcccgg agcgcagcac gtccgcgact tccagccgca tcgcgtcgcg
     6421 gtcggcctcg gcggccatga gctcgtggcg gtccatgccg accatcgccg caggcgcgtg
     6481 gccgaagtgg cgcgcgaagg cgggattgac gtagaccagc gtgccgtccc ggcgcgcgag
     6541 cgagacgagc tcgctctgat cctccaccag cgcgcggtgg cgctgctcgc tgagcgcgag
     6601 gtcgtgggcg atgcggcggc gcacggccag cgcctgcacg agctggccga tcgccagcgc
     6661 gacgaacacg agcagcgagc ccaccgccag caaaagcagg cgctggacgc gggcggcggc
     6721 ggcggcgatg tccacgccga tgcggtcgcg ggcgtcgcgc gcggcgtcga gcagctcgcc
     6781 cacgtccgcc cgggtgcgca gcatcaggtc gcggccgcgg tcgctgagca tcaacgcgag
     6841 ggcacggtcg tgccggccgc cggcctgcag gcgaacggtc tcgtccatct cggcgagctt
     6901 gtcggtcacc agctggcgca tctcctgcga cttgcgcgcg acgccggggt cggctacctc
     6961 ggcgcggccg tccagcgagg ccagcagcgc cggcatgcgg gcgacggccg cgcggtacgg
     7021 gcccaggtag cgctcgtcgt tcgtgagcag gtagccgcgc tggccggtct cggcgtcgag
     7081 cagggccacc gtcacgtcct gcagcgcccg caggcgcagc gagagggcgg cctgccgcga
     7141 cacctgctgc agcgtgcgct cggcctcggt cgcgaaccgt gccgtgaccc cgaccaggcc
     7201 ggcgagcgac agcgcgagcg ccgccagcgt cagcctcagc caggtcctgc gtcgcgtgtg
     7261 cgcctcgttc gggtcgaccc ggccggccat ctgcacgatt ccaatccttc ggcgtggtct
     7321 ccccgtcgtt tttcccgcga gtgtagggga tgccggcgcc cctgtcgggc ggcgccggcc
     7381 acgtcaacgc tgtcagttcg ccacggcgcg gatgtagatc gacgcgctgg ccttgcacag
     7441 ctggctggag gaggcgggtt gcttgggcag cgtcaggtac gaagcctgca ccgagctggg
     7501 cagcggcgtg ggcaggtgca ggtggaagcg cacctcctcg agccaggact tgccgcccga
     7561 gctgacgcac aggtactcga tgtcgctctt cgcgtaggtg ccgaagtcca gcgcggcggc
     7621 gctttgcagc tgcgacagcg tcacgtggcc gccgacgttg gcggcgaccg tcgcgccgag
     7681 gttgctcgcg ttgacgctgc ccagcatgcc cagcgtcggg gcgaagaagt tctgctgcga
     7741 caggccggag caggtgccgt gctccgtcca ttcatgccgc tcgaggtagg acatcgtgcc
     7801 cggctcgtcg acctggagca gcgactcggt ggcgggttgc agcgtcaccg cgggcagcat
     7861 cgaccactcg ccggcctggt cctccgactt ctgggccgcg ctcacgccgc agtaggagcc
     7921 gttctgcggc cacaggccgt gcagcgagaa ctggtgcgcg tcggcgcggc tgggcgtttc
     7981 gcttgtgcat tccggcttgc cggatttggt caggcagaac gcgggctccc agctgatggc
     8041 cagggcgtgg gtctccgcgg gcgcggaggc gcgcgcggcc ggggcgacgg ccgaggcggc
     8101 ggcgaggccg gcgatcgcga gcgcgcggag gaagggcagg gcggtcgatg cggggagggc
     8161 gcgcgagacg cgcgaggaac gagcgtgagt catggctggg atcccgttgt gttgatggtt
     8221 ggagatcgtc cgtccggctc gtgaccggcg gcgatcggcg cgaatttcag caccgcgcct
     8281 tgtcaaatgg cggtcatacg gtaaggcgat gtaagcgcgc ggggggcgtt tgcgggtata
     8341 cgtctacggg tatgcccggc atgcgccgcg gcggctgtcg ccaagcttgc cggccgggcc
     8401 gccgggccgc tcgtggacaa tcgtccgatg ccccgcccga ccgcccccgg ccatgcctga
     8461 cgacacgctc gtctccgccg acctgcgcct ggcgggccgc ctggacgccc gcttcttcgc
     8521 gctgctggag gcgatcgccg ccacggggtc gatcaaccgc gccgcgcgca cggcagggct
     8581 gagctaccgc ggtgcctggc tgatcctcga caacgcggcc aacctggcgc gctcgccgct
     8641 ggtcgagagc gcgatcggcg gcagcggcgg cggcggcacg cgcctgaccg acatggcccg
     8701 cgagctgatg gccgcgtggc gcgacctgca atcccggcac cacgccttcc tgcgcgagca
     8761 ggaggcctgg ctgctgcaac ggccggcgct cgccggcgcc ctccggagga tggccatgaa
     8821 gacttccgca cgcaaccagt tcgccggcac gatcgtcgac ctcgcccgcg ggccggtcag
     8881 caccgaggcc acgctggagc tggcgggcgg ccagcgcatc acggccgccg tgacgacaca
     8941 ggccgccgag cggatgggcc tcgcggccgg gcaggaggcg atcgcgctga tcaaggcctc
     9001 ggcgatcgtg ctggtggccg acttcgcggg ctacgtgctg tccgcgcgca accagctcgc
     9061 cggcacggtc tcgcgcatcg agcgcggcgc cgtgtcctcg ctcgtggcac tgacgctgcc
     9121 tggcggcacg gtcgtcacgg cctcggtgac caacgacgcc gtcgacgcgc tcgacctcgc
     9181 ggtgggccac gcggcgagcg cggtcttcaa ggcctactcg gtcctgctgg ccgtcgccgc
     9241 gaagtgatcg tcagcgcagc agcttgaggc tgccggtcag cgaccgcacc cacttcccgg
     9301 gcccggcgat cccgaggccg tcgatcgcct cgccggccag gcggcggcgc gggaactcgg
     9361 ccaggtagtc ctcgaagcgg tgcaggctgc gtgcgaagcg cgggaacggc gcgacgacgc
     9421 ccccttcggg caccacctgc gcgcgcgcga gcagcgcggc catcgccgcg ggcgtggcct
     9481 gcaggatggg cacgggccgg ttggagctgg tgcgcacggc acggccgtcg gcatcgacga
     9541 ggacgtcgcc gccgagcacc gtcgccgcgg cgcccaggcc gatgccgatc gccgcgaccg
     9601 tgttggcgac caggccggcc ggcagcgacg ggtcgacgac gatggcgatg cgcaggtcgt
     9661 cgccgccggt cgacgacccc tggggctcac gacccgcgtc gactccggcc tcggggaatg
     9721 gggcagcgct cattcggtgg tcgccccggc gatggtcagc gcgaaggtcg acacgctcgc
     9781 cgcggtcacc gcctgcggct gcagcagcgc gagctcgtcg cgcgggctct cgaggaagcg
     9841 cagtt
//
LOCUS       DASVLN010000005         2788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_215237, whole genome shotgun sequence.
ACCESSION   DASVLN010000005 DASVLN010000000
VERSION     DASVLN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215237"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VF453_00120"
     CDS             complement(<1..266)
                     /locus_tag="VF453_00120"
                     /inference="COORDINATES: protein motif:HMM:NF045455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686071.1"
                     /translation="MPAMIPFFAPDTRHLGWFDGQHVFDLALDWVAVHDRGNLFSTRS
                     WQWLGPLHEGSFLDREGRPVAWLLGAAPAGRLAPPAPMSPSRPLR"
     gene            complement(284..2686)
                     /locus_tag="VF453_00125"
     CDS             complement(284..2686)
                     /locus_tag="VF453_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7686072.1"
                     /translation="MHVQYSDANPDNPASARLLAGLNAEQLAAVTLPARPALILAGAG
                     SGKTRVLTTRIAWLLQTGQVSPGGIMAVTFTNKASKEMQARLAAMLPVNVRGMWIGTF
                     HGLCNRFLRAHWKLAGLPQGFQILDSSDQLSAVKRIVKAMNLDEERFVPKQVAWFIAG
                     AKEDGLRPKDIEVHDGQTRTMVEIYEAYEAQCQREGVVDFAELLLRAFELMRDHEAVR
                     EHYRHRFQHILVDEFQDTNRLQYAWLKMFAGPHGAVFAVGDDDQSIYGFRGALVGNMA
                     DFEREFRVDKVIKLEQNYRSFGNILDAANHLIDRNSKRLGKNLRTDQGPGEPVRVFEA
                     TSDFAEAQWFLDEAQQLHRAGTPRAEIAVLYRSNAQSRVIESALFNAGIPYRVYGGLR
                     FFERAEVKHALSYLRLIENPNDDTSFLRVVNFPARGIGARTIEQLQDAARTSGRSLHQ
                     SVGSVAGKGGANLAQFNALVDGLRERTRGLTLREIIEQMLADTGLIEFYRADKDGADR
                     VENLEELTNAAEAFVTQEGFGRDAVALPVDELGPGTLASRNTSRDVPGLMLDAPVTPD
                     AETGEIMSPLAAFLTHASLEAGDNQAQAGQDAIQLMTIHSAKGLEFDSVFITGLEEGL
                     FPHENSLQSHDGLEEERRLMYVAITRARKRLYLSFSQTRLLHGQTRYNVKSRFVDELP
                     EANLRWITPRNQGFGSGYAREYQQAWQRGTGLGSVVGAGRRDDVGGPPPLPAAMEQGR
                     SATTGGHRFRSGQSVFHTKFGEGVIVTLEGSGEEARAQVNFGRHGTKWLALGVAKLQP
                     ID"
BASE COUNT          368 a          963 c         1017 g          440 t
ORIGIN      
        1 cgcagcggcc gcgacgggct catcggcgcc ggcggtgcga gccggcccgc gggcgccgcg
       61 ccgagcagcc aggcgaccgg ccgcccttcg cggtcgagga acgagccctc gtgcagcggc
      121 ccgagccact gccagctgcg cgtcgagaac aggttgccgc gatcgtgcac cgcgacccag
      181 tcgagcgcga ggtcgaacac gtgctggccg tcgaaccagc cgaggtggcg ggtgtccggc
      241 gcgaagaacg ggatcatggc tggcatgcgg tcgccccggc tcgtcagtcg atcggctgca
      301 gcttggcgac gcccagcgcc agccacttcg tgccgtggcg gccgaagttg acctgcgccc
      361 gggcctcttc gccggagccc tccagcgtga cgatcacgcc ctcgccgaac ttggtgtgga
      421 agaccgactg gccggagcgg aagcggtggc cgccggtcgt ggcgctgcgg ccctgctcca
      481 tcgcggcggg cagcggcggc ggccccccca cgtcatcgcg gcggccggcg ccgaccaccg
      541 acccgaggcc ggtgccgcgc tgccaggcct gctggtactc gcgcgcatac cccgagccga
      601 agccctggtt gcgcggcgtg atccagcgca ggttggcctc cggcagctcg tcgacgaagc
      661 ggctcttgac gttgtagcgc gtctggccgt gcagcaggcg cgtctggctg aagctgaggt
      721 acaggcgctt gcgggcgcgc gtgatcgcga catacatcag ccgccgctcc tcctccaggc
      781 cgtcgtgcga ctgcagcgag ttctcgtgcg ggaacaggcc ttcctcgagg cccgtgatga
      841 agactgagtc gaactccagg cccttggccg agtggatcgt catcagctgg atcgcgtcct
      901 ggcccgcctg ggcctggttg tcgccggcct cgagcgacgc gtgcgtgagg aaggccgcga
      961 gcggggacat gatctcgccg gtctcggcgt cgggcgtgac cggcgcgtcg agcatgaggc
     1021 ccgggacgtc gcgcgacgtg ttgcggctgg cgagcgtgcc ggggcccagc tcgtcgaccg
     1081 gcagcgccac cgcatcgcgg ccgaacccct cctgcgtgac gaaggcctcg gcggcgttgg
     1141 tcagttcctc gaggttctcg acgcggtcgg cgccgtcctt gtcggcgcgg tagaactcga
     1201 tcaggccggt gtcggcgagc atctgctcga tgatctcgcg cagcgtcagg ccgcgggtgc
     1261 gctcgcgcag cccgtcgacg agcgcgttga actgcgcgag gttcgcgccg cccttgccgg
     1321 cgacgctgcc gacgctctgg tgcaggctgc ggccgctcgt gcgcgccgcg tcctgcaact
     1381 gctcgatcgt gcgcgcgccg atgccgcgcg ccgggaagtt gacgacgcgc aggaagctcg
     1441 tgtcgtcgtt cgggttctcg atcaggcgca ggtaggacag cgcgtgcttg acttcggcgc
     1501 gctcgaagaa gcgcagtccg ccgtacaccc ggtagggtat gccggcgttg aagagcgccg
     1561 actcgatcac ccgcgactgc gcgttgctgc ggtagagcac cgcgatctcc gcgcgcgggg
     1621 tgccggcgcg gtgcagctgc tgcgcctcgt ctaggaacca ctgcgcctcg gcgaagtcgc
     1681 tcgtcgcctc gaacacgcgc accggctcgc ccgggccctg gtcggtacgc aggttcttgc
     1741 cgaggcgctt cgagttgcgg tcgatcaggt ggttggcggc gtccaggatg ttgccgaagc
     1801 tgcggtagtt ctgctccagc ttgatgacct tgtcgacgcg gaactcgcgc tcgaagtcgg
     1861 ccatgttgcc cacgagggcg ccgcggaagc cgtagatgct ctggtcgtcg tcgcccaccg
     1921 cgaagaccgc cccgtgcggc cccgcgaaca tcttcagcca ggcgtactgc aggcggttcg
     1981 tgtcctggaa ctcgtcgacg aggatgtgct ggaagcgatg ccggtagtgc tcgcgcacgg
     2041 cctcgtggtc gcgcatcagc tcgaaggcgc gcagcagcag ctcggcgaag tcgacgacgc
     2101 cctcgcgctg gcactgggcc tcgtaggcct cgtagatctc gaccatcgtg cgcgtctggc
     2161 cgtcgtgcac ctcgatgtcc ttcggccgca ggccgtcctc cttggcaccg gcgatgaacc
     2221 aggcgacctg cttggggacg aagcgctcct cgtcgaggtt catcgccttg acgatgcgct
     2281 tgacggcgga aagctggtcg gacgagtcga ggatctggaa gccctgcggc agcccggcga
     2341 gcttccagtg cgcgcgcagg aagcggttgc acaggccgtg gaaggtgccg atccacatgc
     2401 cgcgcacgtt gaccggcagc atcgcggcga gccgcgcctg catctccttg gatgccttgt
     2461 tggtgaaggt gacggccatg atgccgccgg gcgagacctg cccggtctgc agcagccagg
     2521 cgatgcgggt ggtgagcacg cgcgtcttgc ccgagcccgc gccggcgagg atgagcgcgg
     2581 gccgcgccgg cagcgtcacg gcggcgagct gctcggcatt gagcccggcc agcaggcggg
     2641 cgctggcagg gttgtcggga ttggcgtcag aatactggac gtgcatccac ccattctaga
     2701 agtccgcggc cggctcgccc gcgcatccgc cctcgccgcg cgcggggttc tgggcctcgc
     2761 gctcgccgcc gccgccgtcg ccgcctcg
//
LOCUS       DASVLN010000006         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_107852, whole genome shotgun sequence.
ACCESSION   DASVLN010000006 DASVLN010000000
VERSION     DASVLN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107852"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..487
                     /gene="fdx"
                     /locus_tag="VF453_00130"
     CDS             149..487
                     /gene="fdx"
                     /locus_tag="VF453_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEX7686073.1"
                     /translation="MTTIKILPHATLCPEGKTISAAPGTSVCEALLENGVPIEHACDM
                     SCACTTCHVIVREGYASLGEPEETEEDLLDRAWGLTPQSRLSCQAIVATTDLVVELPK
                     YTINHARENH"
     gene            complement(553..831)
                     /locus_tag="VF453_00135"
     CDS             complement(553..831)
                     /locus_tag="VF453_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686074.1"
                     /translation="MKQYKLTAWPELPARFQKTTMRRVVSELSQRFVSTRDLARSCGA
                     PAREVDELVEHLNREGLLMSRDAPQTVGGAANWREWGPVVAMRELVRR"
     gene            999..2030
                     /gene="tsaD"
                     /locus_tag="VF453_00140"
     CDS             999..2030
                     /gene="tsaD"
                     /locus_tag="VF453_00140"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7686075.1"
                     /translation="MIVLGIESSCDETGVALVEAGPAGLPRLRGQALHSQIRMHQAYG
                     GVVPELASRDHVRRVLPLLEVALGEAGLGRRDVDVVAFTRGPGLAGALLVGAGVACAL
                     AASLGRPTLGVHHLEGHLLSPFLSADPPEFPFVALLVSGGHTQLMRVDGVGRYEMLGE
                     TIDDAAGEAFDKSAKLLGLGYPGGPALAKLAEQGDPEAFALPRPLLHSGDLDFSFAGL
                     KTAVLTAVKRLGDDATTQQRADLAASTQAAICEVLVKKALRALQQMRLSRLVVAGGVG
                     ANTRLRAELDAAAARRGHRVHYPELALCTDNGAMIALAGAMRLQVEPGRATPDYAFDV
                     RPRWPLAQA"
BASE COUNT          284 a          780 c          778 g          293 t
ORIGIN      
        1 ccgccaccgc cgccgagccg cgcgccgtcg aggccgcggt cgaggcgctg gccgcgggca
       61 ccgagggctt cgccgcggcg cgcatgaacc gcggcatccg cgaggcgctc gccgggcgca
      121 acgtcaacga catctgaaga ccacctgcat gacgacgatc aagatcctcc cgcacgcgac
      181 gctctgtccc gagggcaaga cgatctcggc ggcgccgggc acctccgtgt gcgaggcgct
      241 gctggagaac ggcgtgccca tcgagcacgc ctgcgacatg agctgcgcct gcacgacctg
      301 ccacgtcatc gtgcgcgagg gctacgcctc gctcggcgag cccgaggaga cggaggagga
      361 cctgctcgat cgcgcctggg gcctgacgcc gcagtcgcgg ctgtcgtgcc aggccatcgt
      421 cgcgacgacc gacctcgtcg tcgagctgcc gaagtacacg atcaaccacg cgcgcgagaa
      481 ccactgaggc atgaaaaaac ggcccgcggg ccgttcgtcc gatgccccgc cgacgcggcg
      541 ggcgagggcg cctcagcgcc gcaccagctc gcgcatcgcc acgaccgggc cccactcgcg
      601 ccagttggcg gcaccgccga cggtctgcgg cgcgtcgcgc gacatcagca ggccttcgcg
      661 gttcaggtgc tcgaccagct cgtcgacctc gcgcgccggt gcgccgcagc tgcgggccag
      721 gtcacgcgtg ctgacgaagc gctgggacag ctcgctcacg acgcgacgca tggtcgtctt
      781 ctggaaccgg gcgggcagtt cgggccaggc cgtgagcttg tattgcttca tgttcttgga
      841 ggcttttcgg aggtcttgct atcggcccgc agccgcggga tttcagccgc tcgcgcgcgc
      901 ggtcgacccg ctttgtaccg cacgagtgcg cccgcggcaa accgggatgt cgccgtttgg
      961 acccgatgag tcgacaaatg cacacataca ctcgccccat gatcgtcctc ggcatcgaat
     1021 cctcctgcga cgagaccggc gtcgcgctcg tcgaagcggg gcccgcgggc ctgccgcggc
     1081 tgcgcgggca ggcgctgcac agccagatcc gcatgcacca ggcgtacggc ggcgtggtgc
     1141 ccgagctcgc ttcgcgcgat cacgtccgcc gcgtgctgcc cttgctggag gtcgcgctgg
     1201 gcgaggccgg gctcgggcgc cgggacgtcg acgtcgtcgc gttcacgcgc gggcctggcc
     1261 tggcgggggc gctgctcgtc ggcgcgggcg tcgcctgcgc gctcgcggcg tcgctcggcc
     1321 ggcccacgct gggcgtgcac catctggagg gccacctgct gtcgcccttc ctctcggccg
     1381 acccgccaga gttcccgttc gtcgcgctgc tcgtctcggg cggccacacg cagctgatgc
     1441 gggtcgacgg cgtgggccgc tacgagatgc tgggcgagac catcgacgac gcggccggcg
     1501 aggccttcga caagtcggcc aagctgctcg gcctcggcta tccgggcggc ccggcactgg
     1561 cgaagctggc ggagcagggc gatcccgagg ccttcgcgct gccgaggccg ttgctgcaca
     1621 gcggcgacct ggacttctcc ttcgccgggt tgaagacggc cgtgctgacc gccgtgaagc
     1681 ggcttggcga cgacgcgacc acacagcagc gcgccgacct cgccgccagc acgcaggccg
     1741 cgatctgcga ggtgctggtg aagaaggcgc tgcgcgcgct gcagcagatg cggctcagcc
     1801 ggctcgtcgt tgccgggggc gtcggcgcga acacgcggct gcgtgcggag ctcgatgccg
     1861 ccgccgcgcg gcgcggccat cgcgtgcact atcccgagct cgcgctgtgc accgacaacg
     1921 gcgcgatgat cgccctggcc ggcgcgatgc ggctgcaggt cgagcccggc cgtgcgacgc
     1981 cggactacgc cttcgacgtg cggccgcgct ggccgctggc gcaggcctga acgggcgaag
     2041 cccccgcggc cggagggcgg cgagggctcc gtggattgcg accgacgccg cgcgggcgcc
     2101 gcgtcgatca gtcgacggcc agctgggcgc tggcc
//
LOCUS       DASVLN010000007         3813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_484019, whole genome shotgun sequence.
ACCESSION   DASVLN010000007 DASVLN010000000
VERSION     DASVLN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484019"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /locus_tag="VF453_00145"
     CDS             <1..1878
                     /locus_tag="VF453_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7686076.1"
                     /translation="PGVLLVLLLAAAGVVVRRVLLPMRRLDAAAAAIRRGDLSHRIAS
                     TSQDELGELAREFDAMTAALVESDRQRDRSEQQLRDITDNLPALVAYIDPGHQYRFAN
                     DKYREWLGHDPFTMVGRHVAEVLGESHYQAAKEALDQALRGERSRWSRTLRRGGRETH
                     LLAEYIPDVAADGRVRGVYALTIDITERRQAELLMQRSEQRMVELANAIPAMVGWFDM
                     DEVCHYANDAGLRSLGLTQADVPGITMRAALGETIYAQHEPYVREALQGRRARLEGSI
                     PFEGREAHFQAHLIPDRVAGGAQRGYYLMTFDITATKEAEARRARAERQLRAIADNLP
                     VAITYLDREQRYRFVNRTGLQWIGKPGGEVLGQRIGDVLRPAAYELRRAALTRALGGE
                     RVEFEVDPPPLDGVERVLHYVYLPDIQADGSVAGVYGLGSDVTALKKVERQLSLLVRS
                     DTLTGLANRYQFNEALPLALARARRAGNSLALMYLDIDRFKSINDTLGHAAGDEVLKA
                     FAQRLVQSVRATDTVARLGGDEFVIVLEGLKLPDEPGVVARKIIANVTKPLAVAGQPL
                     ALTTSIGIAFCSRVTMAEMRNAEGLLARADEALYAAKAAGRATWRVLVDGVATAEEAT
                     T"
     gene            1932..3686
                     /locus_tag="VF453_00150"
     CDS             1932..3686
                     /locus_tag="VF453_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7686077.1"
                     /translation="MTPDPAIVPTAGDLAGLPPEEARRLAVLRELGVLDTLPEPVFDA
                     IVASAARACGVPMATISLIDLNRQWFKARVGLDVPETPRKIAFCDHAIRSDRLMEVPD
                     ATRDERFAANPLVTGHPDVRFYAGAPIRLEDGSRVGTVCVIDRVPRALDDRQRETLES
                     LAAIVASLLDQRRRLLQATDRLAETERRQRRLYQASPAILQSVGPDGRVLDVSDPWLA
                     LMGYERDEVVGRMSSDFMSEASRRRARQVVLPELFRTGRCDRVEYQFVHRDGHLIDVL
                     LTARLERDDQGNPLRSAAVLEDVSASKRLQAELDRTTAHLDAVVDNMPALAGYWDRDA
                     VTRFVNRDFQAAVGLPADRLVGCGLAQVVAAVDPVGYETIAPRVAAVLEGRRQEFECA
                     LLTTSGLRQLRVTFVPAQPEDGRVDGFYGTFFDITGMKALELRQRESEHRYRLLFDHL
                     DSAHALHAVILDKAGKPVDYRFLAMNPAFSELTGLDPATTINARATEVFPHIATDPAD
                     WIGRFGRVALTGEPAHIEQQSASGRWWDMVVYRPGPGQFAVIAREVSHVRRLERELER
                     AQAQVRELQERLAGAVDR"
BASE COUNT          520 a         1448 c         1349 g          496 t
ORIGIN      
        1 cccggcgtgc tgctggtgct gctgctggcg gccgccggcg tcgtggtgcg gcgcgtgctg
       61 ctgccgatgc gtcgcctgga cgccgccgcc gccgcgatcc gccgcggcga cctgagccac
      121 cgcatcgcga gcacctcgca ggacgagctc ggcgagctcg cgcgcgaatt cgacgcgatg
      181 accgccgcgc tggtcgagag cgaccgccag cgcgaccgct ccgagcagca gctgcgcgac
      241 atcaccgaca acctgccggc gctggtcgcc tacatcgacc ccggccacca gtaccgattc
      301 gccaacgaca agtaccgcga gtggctgggc cacgacccgt tcacgatggt cggccgccat
      361 gtcgccgagg tgctgggcga gtcgcactac caggccgcca aggaggcgct ggaccaggcg
      421 ctgcgcggcg agcgctcgcg ctggtcgcgc accctgcgcc gcggcgggcg cgagacgcac
      481 ctgctggccg agtacatccc cgacgtcgcg gccgacggcc gcgtccgcgg cgtctacgcg
      541 ctcacgatcg acatcaccga gcgccgccag gccgagctgc tgatgcagcg cagcgagcag
      601 cgcatggtcg agctcgccaa cgccatcccc gcgatggtcg gctggttcga catggacgag
      661 gtctgccact acgccaacga cgccggcctg cgctcgctcg ggctgacgca ggcggacgtg
      721 cccggcatca cgatgcgcgc ggcgctcggc gagaccatct acgcccagca cgaaccctac
      781 gtgcgcgagg cgctgcaggg ccggcgggcg cgcctggagg gcagcatccc gttcgagggc
      841 cgcgaggcgc acttccaggc ccacctgatc cccgaccgcg tcgccggcgg cgcgcagcgc
      901 ggctactacc tgatgacctt cgacatcacc gcgacgaagg aggccgaggc ccggcgcgcg
      961 cgtgcggagc gccagctgcg cgccatcgcc gacaacctgc cggtggccat cacgtatctt
     1021 gaccgcgagc agcgctaccg cttcgtcaac cgcaccggcc tgcaatggat cggcaagccg
     1081 ggcggcgagg tgctcggcca gcgcatcggc gacgtgctgc gcccggcagc ctacgagttg
     1141 cgccgggcgg cgctcacccg cgcgctgggg ggcgagcgcg tcgagttcga ggtcgatccc
     1201 ccgccgctgg acggcgtcga gcgcgtcctg cactacgtct acctgcccga catccaggcc
     1261 gacggaagcg tggccggcgt ctacggcctg ggctcggacg tcaccgcgct gaagaaggtc
     1321 gagcgccagc tgagcctgct cgtgcgctcc gacacgctca ccggcctggc caaccgctac
     1381 cagttcaacg aggcgctgcc gctcgcgctc gcgcgcgcgc gccgggcggg caactcgctg
     1441 gcgctgatgt acctggacat cgaccgcttc aagtccatca acgacacgct gggccacgcc
     1501 gcgggcgacg aggtgctcaa ggccttcgcg cagcggctcg tgcagagcgt gcgcgccacc
     1561 gacaccgtcg cccgcctggg cggcgacgag ttcgtcatcg tcctcgaggg cctgaagctg
     1621 cccgacgagc cgggcgtcgt cgcgcgcaag atcatcgcca acgtgacgaa gccgctggcg
     1681 gtcgcgggcc agccgctcgc cctcacgacc agcatcggca tcgcgttctg ctcgcgcgtg
     1741 acgatggccg agatgcgcaa cgccgagggc ctgctcgcgc gcgccgacga ggcactctac
     1801 gccgccaagg ccgcgggacg cgccacgtgg cgggtgctgg tggacggcgt cgcgaccgcg
     1861 gaggaggcga ccacgtgacg gccagggtct cctccgctga tcccgccgcg ccggccgctc
     1921 ccgaattcgc catgaccccc gatcccgcca tcgtcccgac cgccggcgat ctcgccggcc
     1981 tgccgcccga ggaggcccgg cgcctggcgg tgttgcggga gctgggcgtg ctcgacacgc
     2041 tgcccgagcc ggtgttcgac gccatcgtcg cctcggccgc gcgcgcctgc ggcgtgccga
     2101 tggccaccat ttcgctgatc gacctgaacc gccagtggtt caaggcccgc gtgggcctcg
     2161 atgtgcccga gacgccgcgc aagatcgcgt tctgcgacca cgcgatccgc tccgaccggt
     2221 tgatggaggt gcccgacgcg acgcgcgacg agcgcttcgc cgccaacccc ttggtcactg
     2281 gccatccgga cgtgcgcttc tacgccggcg cgccgatccg gctggaggac ggctcgcgcg
     2341 tcggcacggt gtgcgtgatc gaccgcgtgc cgcgcgccct cgacgaccgc cagcgcgaga
     2401 cgctggagag cctggccgcg atcgtcgcga gcctgctcga ccagcgccgc cgcctgctgc
     2461 aggccaccga ccgcctggcc gagaccgagc ggcgccagcg ccggctctac caggcgtcgc
     2521 cggcgatcct gcagtcggtc gggccggacg gccgcgtcct cgacgtcagc gacccctggc
     2581 tcgccctgat gggctacgag cgcgacgagg tcgtcggccg catgtcgtcg gatttcatga
     2641 gcgaggcctc ccggcggcgc gcgcgccagg tcgtgctgcc ggagctcttt cgcaccgggc
     2701 gctgcgaccg cgtcgagtac cagttcgtgc accgcgacgg ccacctgatc gacgtgctgc
     2761 tgaccgcacg cctggagcgc gacgaccagg gcaacccgct gcgctcggcg gccgtgctgg
     2821 aggacgtgtc ggccagcaag cgcctgcagg cggagctcga ccgcaccacg gcgcacctgg
     2881 acgccgtcgt cgacaacatg cccgcgctcg cgggttactg ggaccgggac gcggtcacgc
     2941 gcttcgtcaa ccgcgacttc caggccgcgg tcggcctgcc ggcggaccgg ctggtcgggt
     3001 gcggactcgc gcaggtcgtc gcggcggtcg acccggtggg ctacgagacg atcgcgccgc
     3061 gcgtggccgc ggtgctggaa ggccggcgcc aggagttcga gtgcgcgctg ctcacgacct
     3121 ccggcctgcg ccagctgcgc gtcaccttcg tgcccgcgca gcccgaggac ggccgcgtcg
     3181 acggcttcta cggcaccttc ttcgacatca ccggcatgaa ggcgctcgag ctgcgacagc
     3241 gcgagagcga gcaccgctac cgcctgctgt tcgaccacct cgacagcgcg cacgcgctgc
     3301 atgcggtgat cctcgacaag gccggcaagc cggtggacta ccgcttcctg gccatgaacc
     3361 cggccttctc agagctgacg ggcctcgacc cggcgaccac gatcaacgcg cgcgcgaccg
     3421 aggtcttccc gcacatcgcg accgatcccg cggactggat cggccgcttc ggccgtgtcg
     3481 cgctcaccgg cgagcccgcg cacatcgagc agcagtcggc gagcggccgc tggtgggaca
     3541 tggtcgtcta ccgtccgggg ccgggccagt tcgccgtcat tgcgcgggag gtctcgcacg
     3601 tcaggcgcct ggagcgcgag ctcgagcgcg cgcaggctca ggtgcgcgag ctgcaagaga
     3661 ggctggcggg agcggtcgat cgctgagccc gtctgatcgg cggcccgccg cgcgggcgca
     3721 tccgagccgt cacgggcagg atgacgattc cgcccgcggg tcgcgcgcga ccggccggcc
     3781 gcgcgcggcg cctcactcgc ccgtgccgcc ctc
//
LOCUS       DASVLN010000008         1742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_107958, whole genome shotgun sequence.
ACCESSION   DASVLN010000008 DASVLN010000000
VERSION     DASVLN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107958"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..720)
                     /locus_tag="VF453_00155"
     CDS             complement(163..720)
                     /locus_tag="VF453_00155"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HEX7686078.1"
                     /translation="MSRLARLLVLALLAAGALVAQAQTHPAPGLWEEQVTVKSDDAQM
                     EAAMARMKERLAAMPPDQRRIVEAQMAARGVGMGGGAHSVRVCISKEQADRGFVPDRN
                     DDGKCTRQELSRSGNEIDFAYSCNEGETQVTGRGSAVFGDSKSFSVTTESDIRNPRRP
                     MHMSSRIVGRFVSSDCGDVKPSTMH"
     gene            complement(806..>1742)
                     /locus_tag="VF453_00160"
     CDS             complement(806..>1742)
                     /locus_tag="VF453_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX7686079.1"
                     /translation="ASATAVAAPVDLASWCRRVTAQVPGVAQPACAGSGLVAGDGRSV
                     HGVPLFTRDVLPASGAPRFKVLLLGGIHGDELTAVTLAFDWIARSQAAQRLPAPTARP
                     VATATRGDKPVATMADVAWRFVPLLNPDGLLHAPSTRVNAHGVDLNRNFPTADWARDA
                     QPYWIKRTGRDPRRFPGPQPVSEPESRWLLQQIEQFHPDLIVSVHAPYGLLDFDGPPP
                     VPRRLGSLQLDPVGVYPGSLGNYGGLVKQLPVMTLELKNARQVPPAELAAMWVDLQHW
                     IADRLAHPHAQAQADPRGAATPTLAERARVARSRD"
BASE COUNT          217 a          605 c          686 g          234 t
ORIGIN      
        1 gccggcgcaa cgatgttcgg cgccgtgatc gcgggctcgc cggccggcga cctcgcgctg
       61 gcgggccagg gcacgagctg ggtgccgatg tgggcgatgg cgtgcgccgc ggcgctgtgg
      121 gcggcctggc gacaggtgcg cagcgcgtga atacccccgc ggtcagtgca tcgtcgacgg
      181 cttgacgtcg ccgcagtcgg acgagacgaa gcgcccgacg atgcgggagc tcatgtgcat
      241 cgggcgacgc gggttgcgga tgtcggactc ggtcgtcacg ctgaacgact tcgagtcgcc
      301 gaagaccgcc gagccgcgtc ccgtgacctg cgtttcgccc tcgttgcagc tgtacgcgaa
      361 atcgatctcg ttgcccgagc ggcttagctc ctggcgcgtg cacttgccgt cgtcgttgcg
      421 gtcggggacg aagccgcggt cggcctgctc cttcgagatg cacacgcgca ccgaatgcgc
      481 cccgccgccc atcccgacgc cgcgggcggc catctgcgcc tcgacgatcc tgcgctggtc
      541 gggcggcatc gcggccaggc gttccttcat ccgcgccatc gccgcctcca tctgcgcgtc
      601 gtccgacttg accgtcacct gctcctccca caggccgggc gcgggatggg tctgcgcctg
      661 cgcgacgaga gcgccggcgg cgagcagggc taggacgaga aggcgggcga ggcgggacat
      721 ggcggctcct ggtcggacgg tgcgccggat tctgcgccgc gtccgggccc gccgacatcc
      781 ccaggagacg ggatgccggg gcgcgtcagt cgcggctgcg cgccacgcga gcccgctcgg
      841 ccagcgtggg cgtcgccgct ccgcgcgggt ccgcctgcgc ctgggcatgc ggatgggcga
      901 ggcggtcggc gatccagtgc tggaggtcga cccacatcgc ggcgagctcg gcgggcggca
      961 cctggcgcgc gttcttcagc tccagcgtca tcaccggcag ctgcttgacg agtccgccgt
     1021 agttgcccag cgagccggga tagacgccca cgggatccag ctgcaggctg ccgagccggc
     1081 gcggcaccgg cggcggcccg tcgaagtcga gcaggccgta cggcgcgtgc acggacacga
     1141 tgaggtcggg gtggaactgc tcgatctgct gcagcagcca gcgcgactcg ggctcggaga
     1201 ccggctgcgg gcccgggaag cggcgcgggt cgcggccggt gcgcttgatc cagtagggct
     1261 gggcgtcgcg cgcccagtcg gcggtcggga agttgcggtt caggtcgacg ccgtgcgcgt
     1321 tcacgcgcgt cgagggcgcg tgcagcaggc cgtccgggtt cagcagcggg acgaagcgcc
     1381 aggcgacgtc ggccatcgtg gcgacgggct tgtccccgcg cgtcgccgtg gccaccgggc
     1441 gggcggtcgg ggcgggcagg cgctgtgcgg cctgcgagcg cgcgatccag tcgaaggcga
     1501 gcgtgacggc ggtgagctcg tcgccgtgga tgccgcccag cagcagtacc ttgaagcggg
     1561 gcgcgccgct cgcgggcagg acgtcgcgcg tgaacagcgg cacgccgtgc accgagcggc
     1621 cgtcgcccgc gacgagtccc gagcccgcgc atgccggctg ggccacgccg ggcacctgcg
     1681 cagtgacgcg gcggcaccag ctcgcgagat cgacgggcgc ggcaacggcc gtggcgctgg
     1741 ca
//
LOCUS       DASVLN010000009         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_376530, whole genome shotgun sequence.
ACCESSION   DASVLN010000009 DASVLN010000000
VERSION     DASVLN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376530"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VF453_00165"
     CDS             <1..492
                     /locus_tag="VF453_00165"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7686080.1"
                     /translation="RGAPSDDRTETAGNALDDSVITTKVKASLVAQTDGKGGGTSVET
                     RHGNVLLSGFVDSQAQADKEVQLAKGVDGVKSVDNKLMVKDGKSTAGNQLDDSVVTVK
                     VKSALMTDEQTRADQISVNTDKGVVQLSGYVGSAGEQAHAAEVARNVEGVQSVVNDTT
                     LKK"
     gene            511..657
                     /locus_tag="VF453_00170"
     CDS             511..657
                     /locus_tag="VF453_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX7686081.1"
                     /translation="MLYTIAVILVVLWLIGMLTSVTLGGLIHILLVIAIIAVLFRLIS
                     GRGV"
     gene            779..1225
                     /locus_tag="VF453_00175"
     CDS             779..1225
                     /locus_tag="VF453_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEX7686082.1"
                     /translation="MSALVIQSAAAPQAPVQPLAAIASYHAHIYFDGAGQRALAMALR
                     TQIGDRFRVTLGRVHDQPIGPHARSMYQVAFDVATFGKFVPWLMLNRRGLTVLVHPNT
                     DNERADHTTHAIWMGEVLEIVGPEHLADGNEAEAPLPTNTAPMLEP"
     gene            complement(1254..1694)
                     /locus_tag="VF453_00180"
     CDS             complement(1254..1694)
                     /locus_tag="VF453_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686083.1"
                     /translation="MTDATPSPPPAAPPDPVVVLAQLADAAGHASTLLFDYRRSNPGA
                     PDLAQALNLEMTLDKRAIELRTQAVAMLGDRVADALAQLQDATRRIDDFLRGVKTTQA
                     RFGVASAVIALAGAALVGDAGGILSAVGNVHDALRTLQDAARTA"
     gene            complement(1691..>1930)
                     /locus_tag="VF453_00185"
     CDS             complement(1691..>1930)
                     /locus_tag="VF453_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686084.1"
                     /translation="PWRLAQQAARDAVAADELEQRRVAAFGRSADALLAAHAALLANF
                     DKLDGAKRLALVSAFVAQVEAIDDDVAELQDKEPT"
BASE COUNT          301 a          712 c          667 g          250 t
ORIGIN      
        1 cgcggggcgc cgagcgacga caggacggag accgccggca atgccctcga cgactcggtc
       61 atcaccacca aggtcaaggc ctcgctggtc gcccagacgg acggcaaggg cggcggcacc
      121 agcgtcgaga cccgccacgg caacgtgctg ctgagcggct tcgtcgacag ccaggcgcag
      181 gccgacaagg aggtgcagct cgccaagggc gtcgacggcg tgaagtccgt cgacaacaag
      241 ctgatggtca aggacggcaa gtccaccgcc ggcaaccaac tcgacgactc ggtcgtcacc
      301 gtcaaggtca agtccgcgct gatgaccgac gagcagacgc gcgccgacca gatctcggtc
      361 aacaccgaca agggcgtcgt gcagctgagc ggctacgtcg gcagcgcggg cgaacaggca
      421 cacgcggcag aagtcgcgcg caacgtcgag ggcgtccaga gcgtcgtcaa cgacaccacc
      481 ctcaagaagt gatcacccag gagaccccac atgctctaca ccatcgccgt catcctcgtc
      541 gtcctctggc tcatcgggat gctgacctcc gtcacgctcg gcggcctgat ccacatcctg
      601 ctcgtcatcg cgatcatcgc ggtgctgttc cgcctcatca gcggacgcgg cgtctgaacg
      661 cgcggcgcgc cgggccggcc cgcgccggcc cggacaagtg cgcacatcgt cacggacggc
      721 gccccgtcgg ccccgcgaag cttgcgggaa gccttcgggg tgccggtaca gtgtcgggat
      781 gtccgccctc gtcatccagt ccgctgccgc gccgcaggcg cccgtgcaac cgcttgcggc
      841 catcgcgagc taccacgccc acatctattt cgacggcgcc ggccagcgcg cgctcgcgat
      901 ggcgctgcgc acgcagatcg gcgaccgctt ccgcgtcacg ctcggccgcg tccacgacca
      961 gccgatcggc ccgcacgcgc gcagcatgta ccaggtcgcg ttcgacgtcg cgaccttcgg
     1021 caagttcgtg ccctggctga tgctcaacag acgtggcctg acggtgctgg tgcaccccaa
     1081 caccgacaac gagcgcgccg accacaccac ccacgcgatc tggatgggcg aggtgctgga
     1141 gatcgtgggc cccgagcacc tggccgacgg caacgaggcc gaggctcccc tgccgacgaa
     1201 cacggcgccg atgctcgagc cctgaatccc ggggcgccgc gccccgaagg cgctcaggcc
     1261 gtccgggcgg cgtcctgcag cgtcctcaac gcgtcgtgga cgttgccgac cgcgctcagg
     1321 atgccgcccg cgtcgcccac cagcgccgcg ccggcgagcg cgatcacggc gctcgcgacg
     1381 ccgaagcgcg cctgcgtggt cttgacgccg cgcaggaagt cgtcgatgcg ccgcgtcgcg
     1441 tcctgcagtt gcgcgagcgc gtcggcgacg cggtcgccca gcatcgccac ggcctgcgtg
     1501 cgcagctcga tggcgcgctt gtccagcgtc atctcgaggt tgagcgcctg cgcgaggtcc
     1561 ggcgcgccgg ggttgctgcg ccggtagtcg aacagcagcg tcgacgcgtg gcccgcggcg
     1621 tcggcgagct gcgcgagcac gacgaccgga tccggcggag cggcgggcgg cggcgatggc
     1681 gtggcgtcgg tcatgtgggc tccttgtcct gcagctcggc gacgtcgtcg tcgatcgcct
     1741 ccacctgggc gacgaacgcg gacacgagcg ccagccgctt cgcgccgtcg agcttgtcga
     1801 aattggcgag caacgccgca tgggcggcca gcagcgcgtc ggcgctgcga ccgaaggcgg
     1861 cgacgcggcg ctgctcgagc tcgtcggcgg ccacggcgtc gcgcgccgcc tgctgcgcga
     1921 ggcgccacgg
//
LOCUS       DASVLN010000010         5727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_215348, whole genome shotgun sequence.
ACCESSION   DASVLN010000010 DASVLN010000000
VERSION     DASVLN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215348"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="VF453_00190"
     CDS             complement(<1..1866)
                     /locus_tag="VF453_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7686085.1"
                     /translation="MPARIEDYALIGNQETVALVARDGSIDWLGMPRFDSPACLCALL
                     GSADNGRWLLRPVADDARVTRRYRDGSMVLETLFETADGAAVVIDFMARRDGVSDLVR
                     VVRGVRGRVAMRTELVVRFDYGLLVPWVSRADDGRLHFVAGPDRLTLATPIDLRGEDF
                     RTVGEFTLSEGEQLDFALTWSPSWRADAPKPDIPAALDQVDGQWRGWCASLARGARPG
                     SPCEAMSAEVWEAVRRSLLTLKALAHWETGGIVAAATTSLPEKIGGPRNWDYRFCWLR
                     DATLTLYALLGVGFVEEADAWRAWLVRAVGGSPDELQIMYGVAGERRLEELELAHLPG
                     YEGSAPVRIGNAAAGQVQLDVYGEVVDALYVARRAGLAPDEASWALECALVCHLETIW
                     DQPDDGIWEMRGGRRHFTHSKVMAWVAFDRAVRSATEFGLDAPVERWSRVRDEIHEQV
                     CTYGFDAERGSFVQSYGSRALDASLLMVALVGFLPADDPRVVGTVAAIERELVHDGLV
                     RRYDSGDGHDGLPPGEGAFLACSFWLVDNYVLQGRLDEAQALFDRLLAWRNDVGLLAE
                     EVDPVGRRQLGNFPQAFSHLALINSATSLASRSGTAHQRATGSSRTERGGPARDVA"
     gene            complement(1996..2071)
                     /locus_tag="VF453_00195"
     tRNA            complement(1996..2071)
                     /locus_tag="VF453_00195"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2036..2038),aa:His,seq:gtg)
     gene            complement(2104..4023)
                     /locus_tag="VF453_00200"
     CDS             complement(2104..4023)
                     /locus_tag="VF453_00200"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX7686086.1"
                     /translation="MFEFVRTHNKIIQVAMGIVLIPSFVFFGVQSYTHSGADQSEAVA
                     EVDGTDITRAQWDAQTRNALERLREQGRNVDPKTLDTPEARRAALDAVIREHVLQAVV
                     EKDRLPVTNDRVNNFIRTSPEFAQLMAMPPEQRKAVLAAQGMTPEILFSRVQAAMQSQ
                     QALVAPSASGFLPAVADKASADAWLGQRAIQWQRFDVKDYLAATTPTEAQIKAYYDDK
                     SHAAEFTAPEQAKIEYVVLDANALKAQVAVTDDALKKYYEDNKANFNAPEERRVSHIL
                     INVAPNASADDVAKAKAKAEAVLAEVRKNPAGFADIAKKESQDAGSAANGGDLDFMKR
                     GAIPGAFSDTMFSMKEGDISDVVRSEAGFHILKLTGIRGGAPKPFEEVRAEVEDQYRT
                     QQARKLYEADSEKFTNTVYEQPDSLQPVVDAFKLARQTATVTRTPAADATGPLASPKL
                     LAAIFASDSLQGKHNTEAIETGPGQLAAARVVQYTAQHLKSFAEVHDQAADALRKSLA
                     AAAAKKDADARIAQAAKDASIALPQVATVGRTVRDSNVPPQVTEAALKADVSKGPTVV
                     GVALPDGSYAAIRVLKALPPAPQDLAQFKSVIGPAYQDAESQALYDAYKARMKTKVDE
                     TRVAKALSSAASAPN"
     gene            complement(4174..4249)
                     /locus_tag="VF453_00205"
     tRNA            complement(4174..4249)
                     /locus_tag="VF453_00205"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4214..4216),aa:Val,seq:tac)
     gene            complement(4314..4586)
                     /locus_tag="VF453_00210"
     CDS             complement(4314..4586)
                     /locus_tag="VF453_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7686087.1"
                     /translation="MNKSELIEHIARQADISKAAATRALEAVIGGVKTTLKKGGSVSI
                     VGFGTFAVTKRKARVGRNPRTKEPIKIKAAKVPKFRPGKGLKDAVE"
     gene            complement(4762..5334)
                     /gene="pgsA"
                     /locus_tag="VF453_00215"
     CDS             complement(4762..5334)
                     /gene="pgsA"
                     /locus_tag="VF453_00215"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7686088.1"
                     /translation="MFFTIPTLLTWARIVAIPLIVGVFYLPIAMPAANLAATVLFVVT
                     ALTDWADGFLARRLNQTSAFGAFLDPVADKFLVCASLLVLVWLQRCDALLALVIIGRE
                     IAISSLREWMAQIGASRSVAVHMLGKLKTTMQMVAIPFLLFHGHLFGLAWLDTQVWGT
                     VLLWIAAVLTIWSMAYYLRKAIPEIRAKAR"
     gene            complement(5393..>5727)
                     /locus_tag="VF453_00220"
     CDS             complement(5393..>5727)
                     /locus_tag="VF453_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686089.1"
                     /translation="QRPATSMDEYRLRAAHRLVAANPKITYLEPAPDPLLAIPVIEVE
                     VNADGSVRHMTVMRVPTQAQDTVRIALAAIERAAPFGDATHCPKPWKFTEVFLFDDDR
                     RFKPRSLD"
BASE COUNT          815 a         1992 c         2056 g          864 t
ORIGIN      
        1 cgcgacatcg cgcgccgggc cgccgcgctc ggtgcggctg ctgccggtgg cgcgctggtg
       61 cgccgtgccg gagcggctcg cgaggctggt cgccgaattg atcagcgcca ggtgcgagaa
      121 cgcctgcggg aagttgccga gctggcgccg tccgacgggg tcgacctctt cggccagcag
      181 cccgacgtcg ttgcgccagg cgagcaggcg gtcgaacagc gcctgcgcct cgtccaggcg
      241 gccctggagc acgtagttgt cgacgagcca gaagctgcac gcgaggaagg cgccctcgcc
      301 gggcggcagg ccgtcgtggc cgtcgccgct gtcgtagcgc cgcacgaggc cgtcgtgcac
      361 gagctcgcgc tcgatcgccg cgacggtgcc gacgacgcgc ggatcgtcgg ccggcaggaa
      421 gcccaccagc gccaccatca acaggctcgc gtcgagcgcg cgcgagccgt aggactgcac
      481 gaagctgccg cgctcggcgt cgaagccgta ggtgcagacc tgctcgtgga tctcgtcgcg
      541 gacgcggctc cagcgctcga cgggcgcgtc caggccgaac tcggtggcgg agcgcaccgc
      601 gcggtcgaac gcgacccagg ccatcacctt cgagtgcgtg aagtggcgcc ggccgccgcg
      661 catctcccag atgccgtcgt ccggctggtc ccagatcgtc tcgaggtggc acacgagcgc
      721 gcattcgagc gcccagctcg cctcgtcggg ggcgaggccc gcgcggcgcg cgacgtacag
      781 cgcgtcgacg acctcgccgt agacgtccag ctgcacctgg cccgccgcgg cgttgccgat
      841 gcgcaccggc gccgagccct cgtagccggg caggtgcgcg agctcgagct cctccagccg
      901 ccgctcgccg gcgacgccgt acatgatctg cagctcgtcc ggactgccgc cgaccgcacg
      961 caccagccac gcgcgccagg cgtcggcctc ctcgacgaag cccacgccca gcagcgcgta
     1021 cagcgtgagc gtcgcatcgc gcagccagca gaagcggtag tcccagttgc gcgggccccc
     1081 gatcttctcc ggcagcgagg tggtcgccgc ggcgacgatg ccgccggtct cccagtgcgc
     1141 gagcgccttg agcgtcagca gcgaacgccg caccgcctcc cagacctcgg ccgacatcgc
     1201 ctcgcacggc gagcccggcc gcgcgccgcg cgcgagcgag gcgcaccagc cgcgccactg
     1261 cccgtcgacc tggtccagcg cggccgggat gtcgggcttg ggggcatcgg cgcgccacga
     1321 cggcgaccac gtgagcgcga aatcgagctg ctcgccctcg gacagcgtga actcgccgac
     1381 ggtgcggaaa tcctcgccgc gcagatcgat cggcgtggcc agcgtgaggc ggtcggggcc
     1441 ggcgacgaaa tgcaggcgac cgtcgtcggc gcgcgagacc cagggcacca gcagcccgta
     1501 gtcgaagcgc acgaccagct cggtgcgcat cgccacgcgg ccgcgcacgc cgcgcacgac
     1561 gcgcacgagg tcggacacgc cgtcgcgccg cgccatgaag tcgatcacga cggccgcgcc
     1621 gtccgcggtc tcgaacagcg tctccagcac catcgagccg tcgcggtagc gccgcgtgac
     1681 gcgcgcgtcg tccgcgaccg gccgcagcag ccagcgtccg ttgtcagccg agcccagcag
     1741 cgcgcacagg caggccgggc tgtcgaagcg cggcatgccg agccagtcga tcgatccgtc
     1801 gcgcgcgacc agggcgaccg tctcctggtt gccgatcaag gcgtagtctt cgatgcgggc
     1861 aggcatgcgg tggctcgggc gggtggcgag acgatcgcga cggcaatcgg cgggccggct
     1921 gccgacgccg gtgtccggcc ccggaaagcg acaggccccg agggcttgcg ccgtcggggc
     1981 ctgggaatca tcggatgggg tggctgatgg gactcgaacc cacgacaacc agaatcacaa
     2041 tctgggactc taccaactga gctacagcca ccaccggagc cttagatcat atcaccgttt
     2101 ccgtcagttg ggcgcggagg cggcgctcga aagcgccttg gcgacgcgcg tctcgtcgac
     2161 cttggtcttc atgcgggcct tgtaggcgtc gtagagcgcc tggctctcgg cgtcctggta
     2221 ggccgggccg atcacggact tgaactgggc gaggtcctgc ggcgcgggcg gcagggcctt
     2281 cagcacgcgg atcgcggcgt agctgccgtc cgggagcgcc acgccgacca cggtcgggcc
     2341 cttggacacg tcggccttca gagcggcctc cgtcacctgg ggcggcacgt tcgagtcgcg
     2401 caccgtgcgg ccgacggtgg cgacctgcgg cagcgcgatc gaggcatcct tggccgcctg
     2461 cgcgatgcgg gcgtcggcat ccttcttcgc ggcggccgcg gcgaggctct tgcgcaacgc
     2521 gtcggcggcc tggtcgtgca cctcggcgaa ggacttcagg tgctgcgccg tgtactggac
     2581 gacgcgggcc gcggcgagct ggccagggcc cgtctcgatc gcctcggtgt tgtgcttgcc
     2641 ctgcagcgag tcgctcgcga agatggccgc cagcagcttg ggcgaggcca gcgggccggt
     2701 ggcgtcggcg gccggcgtgc gcgtgacggt ggcggtctgc ctggccagct tgaacgcgtc
     2761 gacgacgggc tgcaggctgt cgggctgctc gtagacggtg ttggtgaact tctcggagtc
     2821 ggcctcgtac agcttcctgg cctgctgcgt gcggtactgg tcctcgactt cggcgcgcac
     2881 ctcctcgaag ggcttgggcg cgcccccgcg gatgccggtc agcttgagga tgtggaagcc
     2941 ggcctccgaa cgcacgacgt cgctgatgtc gccttccttc atcgagaaca tcgtgtcgct
     3001 gaacgcgccc gggatggcgc cgcgcttcat gaagtcgagg tcgccgccgt tggccgccga
     3061 gccggcgtcc tgcgactcct tcttcgcgat gtcggcgaaa cccgccgggt tcttgcgcac
     3121 ctcggccagg accgcctccg ccttggcctt ggccttggcg acgtcgtcgg ccgaggcgtt
     3181 cggcgccacg ttgatcagga tgtggctgac gcggcgctcc tcgggcgcgt tgaagttcgc
     3241 cttgttgtcc tcgtagtact tcttcagcgc atcgtcggtg accgcgacct gcgccttcag
     3301 cgcattcgcg tccagcacga cgtactcgat cttcgcctgc tcgggcgcgg tgaactcggc
     3361 ggcgtgcgac ttgtcgtcgt agtaggcctt gatctgcgcc tcggtcggcg tggtggccgc
     3421 gaggtagtcc ttgacgtcga agcgctgcca ctggatcgcg cgctggccga gccacgcgtc
     3481 ggccgaggcc ttgtccgcga cggccggcag gaagccgctc gccgaaggcg cgaccagcgc
     3541 ctgctgcgac tgcatcgccg cctgcacgcg cgagaacagg atctcgggcg tcatgccctg
     3601 cgccgcgagc acggccttgc gctgctcggg cggcatcgcc atcagctgcg cgaactccgg
     3661 cgacgtgcgg atgaagttgt tgacgcgatc gttcgtgacc ggcagccggt ccttctcgac
     3721 caccgcctgc agcacgtgct cgcggatgac cgcgtcgagc gccgcgcggc gcgcctcggg
     3781 cgtgtccagc gtcttcgggt cgacgttgcg gccctgctcg cgcaggcgct cgagcgcatt
     3841 gcgggtctgg gcgtcccact gggcgcgcgt gatgtcggtg ccgtcgacct cggccacggc
     3901 ttccgactga tcggcgcccg agtgggtgta gctctgcacg ccgaagaaca cgaacgaggg
     3961 gatgagcacg atgcccatcg cgacctggat gatcttgttg tgcgtccgga cgaattcgaa
     4021 catgggggac cttcagcggg aagctggaat cgtaacgact gaaaacgaca aaggcgaacg
     4081 ccggatgacc gtgttcgcct ggcgggccgg acgccgcggg ggccggtcgc ctcgcgcgtt
     4141 cgcgaggccg gggccgcggg tcttgccggg gagtggtggg tgctgacggg ctcgaaccgc
     4201 cgacctacgc cgtgtaaggg cgctgctcta ccaactgagc taagcaccct ggaaaccttg
     4261 gtgcgccagc tcccgatgcg ggagcgcgag gggtcagtgc aggcgggcgg cggtcactcc
     4321 accgcgtcct tcaggccctt gccggggcgg aacttcggca ccttcgcggc cttgatcttg
     4381 atgggttcct tggtgcgcgg gttgcggccc acgcgggcct tgcgcttggt caccgcgaag
     4441 gtgccgaacc cgacgatcga cacgctgccg cccttcttga gggtggtctt cacgccgccg
     4501 atgaccgcct ccagcgcgcg cgtcgcggcg gccttggaga tgtccgcctg gcgggcgatg
     4561 tgttcgatca gctctgattt gttcacgaag gaaccccctc tcggaaaacg ctgcgatgta
     4621 gtaggcgggc cgtcgacggc accgaagatt acagacccgc cggcctgaca gtgtcaagcg
     4681 aaggcgcgat tctattcgtt aatcggcagg gcgccggaca cctcccggcg cgctcggcgg
     4741 ggcccgcggg ttactcccag gtcagcgtgc cttggcgcgg atctcgggga tcgccttgcg
     4801 caggtagtag gccatcgacc agatcgtcag cacggccgcg atccacagga gcaccgtgcc
     4861 ccagacctgc gtgtcgagcc acgcgagccc gaacaggtgg ccatggaaca gcaggaacgg
     4921 gatcgcgacc atctgcatcg tcgtcttcag cttgcccagc atgtgcacgg ccacgctgcg
     4981 gctggcgccg atctgcgcca tccactcgcg cagcgacgag atcgcgatct cgcgcccgat
     5041 gatcaccagc gccagcagcg cgtcgcagcg ctgcagccag accagcacca gcagcgacgc
     5101 gcagacgagg aacttgtccg ccaccgggtc gaggaaggcg ccgaacgcgg aggtctggtt
     5161 cagccgccgc gcgaggaagc cgtcggccca gtccgtgagc gccgtcacca cgaacagcac
     5221 cgtcgccgcc aggttggccg ccggcatcgc gatcggcagg tagaacacgc cgacgatcag
     5281 cgggatggcg acgatgcgcg cccaggtgag cagggtgggg atcgtgaaga acatatggac
     5341 atgcattctg caccggccgc ccgcggtggc ccgacgcgtg ccggcatccg ggtcagtcga
     5401 ggctgcgcgg cttgaagcgg cggtcgtcgt cgaacaggaa gacctcggtg aacttccatg
     5461 gcttcgggca gtgcgtcgcg tcgccgaagg gcgcggcgcg ctcgatcgcc gccagcgcga
     5521 tcctgaccgt gtcctgcgct tgcgtcggca cgcgcatcac cgtcatgtgg cgcacgctgc
     5581 cgtcggcgtt gacctcgacc tcgatgaccg ggatcgccag cagcgggtcg ggcgccggct
     5641 ccaggtaggt gatcttcggg ttcgccgcga cgagccggtg ggcggcgcgc agccggtact
     5701 cgtccatcga ggtcgcgggc cgctgct
//
LOCUS       DASVLN010000011         6307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_107973, whole genome shotgun sequence.
ACCESSION   DASVLN010000011 DASVLN010000000
VERSION     DASVLN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107973"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF453_00225"
     CDS             complement(<1..192)
                     /locus_tag="VF453_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686090.1"
                     /translation="MARRDYLDFDPTRGYPAGAAIAYECELCGATLPSLPAPPAGECG
                     CGNVGVDIDAGRVRVGVAGT"
     gene            complement(192..3779)
                     /locus_tag="VF453_00230"
     CDS             complement(192..3779)
                     /locus_tag="VF453_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX7686091.1"
                     /translation="MNAPLPDSIRKALESVSLDDKYALETGRAFMSGVQALVRLPMLQ
                     RTRDALAGLNTAGFISGYRGSPLGGYDQALWSARKHLAAQNIVFQPGVNEELGATAVW
                     GTQQLDLYPQSKKFDGVFGIWYGKGPGVDRTCDVFKHANMAGTAKHGGAIAIAGDDHI
                     SKSSTAAHQSDHIFKACGLPVFFPSSVQDILDMGLHAFAMSRFAGVWAGVKTIQEVVE
                     SASSVDVDPQRVKIVLPEDFEMPPGGVHIRWPDPPLEQEARLMDYKWYAALAYVRANR
                     LNHNVVSTPDDRFGLIASGKAYNDLRQALADLGLDDATCRRLGIRIHKVNVVWPLEAT
                     IARDFAQGLKEILVVEEKRQMIEYQLKEQLYNWRSDVRPTVLGKFDETEGDRSGGEWA
                     VPNPGANWLLRPKADLTPAIIAKAVARRLKKLGVPEDVARRMDERIAVIEAKDRALLE
                     LKSDASAQVGDRTPWFCSGCPHNTSTRVPEGSRAVAGIGCHYMVNWMDRSTSTFTQMG
                     GEGVPWVGQAPFTTDRHIFANLGDGTFYHSGLLAIRQSLAAGVNITYKILFNGAVAMT
                     GGQPVDGQIDVPAITRELEAEGVKRIVVVSDVPEKYNAGIRLAPGTTVHHRDQLDAIQ
                     RELREVAGVSVIVYDQTCATEKRRRRKRGTMAPEPRRVVINEAVCEGCGDCSVQSNCL
                     SVEPLETEFGRKRRINQNTCNQDFSCVKGFCPSFVTVEGQLRKPATPKSRAAALPEVP
                     MPTIPAAPQAWGIVVAGIGGTGVITIGSLLGMAAHLEGKGVVTQDAAGLAQKGGATWS
                     HIQIADTPEAILTTKVDAAKADVVIGCEAIVAANPATLQVMREGRTYVALNTHDSPTA
                     GFVGNPDWQFPHGRCETAIAKAVGLELLGAFDADEVAVKLLGDSIFTNPLMLGYAWQK
                     GRIPLGHAALMRSIELNGVQVEKNKQAFEWGRRCAHDLASVQALFAPAHASQVIEFVK
                     RPSLAEVVAKRVEFLTGYQDGAYAARFASAVEAVRAAEAKVSKGTRLTEAVARNLFKL
                     MAYKDEYEVARLHADPAFKAKLAAQFEDGYKLKFHLAPPLTAAKDAQGQLVKKAYGGW
                     MLGAFGMLAKFRGLRGTAFDVFGYTAERRTERALVREYRALVDELVASLSAERLADAV
                     ALASLPEEIRGYGHVKERHLAAVRLKWEKQLFAWRAGARASATPATQAA"
     gene            4024..4506
                     /locus_tag="VF453_00235"
     CDS             4024..4506
                     /locus_tag="VF453_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7686092.1"
                     /translation="MDRYDVALLQELQRDARQSNADLAARIGLSPAPTWRRVRRLEEL
                     GVITGYRAEIDRRRIGLGVLAFVRVDAERDNADATRALEDAIRGLPEVISCHYISGAG
                     TFELQVVVTDLDAFSRWTMQTLFRLPNVKDLHTSFSLGEVKAGAALPLGHLAPGRGTA
                     "
     gene            4801..6291
                     /locus_tag="VF453_00240"
     CDS             4801..6291
                     /locus_tag="VF453_00240"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7686093.1"
                     /translation="MAAPDALLTSEEQAWLADHPVVLYAPERDFPPFSFVDSQGQHRG
                     LSADVLDLVAAHTGLKFQAVAAGDRATNLERLRRREVDLLTSLRPTTEREGYIGFTSA
                     YVSSPAVILRRRGDHRPGDLARMAGEHVAVDRNSATENFLREQAPQVVPVEVDVAAQG
                     LRDLVFGEVDAAVSTLATASFIIERDRLGGIRVAGETGYFSTLTIGYRKDWPMLGRIL
                     EKGLAQITESERAGIIARWIPLSDIAWWQRPEVQRVLGAASIGTGLLMGGLLLWNRAL
                     RRAVAQRTEALQKELAERQRLETRLRTLAEHDPLTGLMNRAALTEALRRSLALAARQK
                     WSVAVVFVDLDKFKAVNDTLGHAAGDELLRQIASRLQGCLRESDLLGRLGGDEFIVVA
                     EALHDGPRNAIELTDKLLTQMKRPFTIEGRPISAGFSAGIAIYPGDGDTPEALVAHAD
                     AAMYQAKQQGRFRAALYQSGTPAVADAATHDGPAVPEPAPGGRESA"
BASE COUNT          867 a         2238 c         2274 g          928 t
ORIGIN      
        1 cgtgcccgcc acgcccacgc gcacgcggcc cgcgtcgatg tcgacgccga cgttgccgca
       61 gccgcactcg cccgcgggcg gagcgggcag cgagggcagc gtcgcgccgc acagctcgca
      121 ttcgtaggcg atcgcggcac ccgcgggata gccgcgggtc gggtcgaagt cgaggtagtc
      181 gcggcgcgcc atcacgcggc ttgcgtcgcc ggcgtcgccg acgccctcgc gcccgcgcgc
      241 caggcgaaca gctgcttctc ccacttcagg cgcaccgccg ccaggtggcg ctccttcacg
      301 tggccgtagc cgcggatctc ctccggcagc gacgcgagcg ccaccgcgtc ggccaggcgc
      361 tcggcgctca gcgacgcgac gagctcgtcg acgagcgcgc ggtattcgcg caccagcgcg
      421 cgctcggtgc ggcgttccgc ggtgtagccg aacacgtcga aggcggtgcc gcgcaggccc
      481 ctgaacttcg cgagcatgcc gaaggcgccc agcatccagc cgccgtaggc cttcttcacg
      541 agctgcccct gcgcgtcctt ggcggccgtc agcggcggcg cgaggtggaa cttgagcttg
      601 tagccgtcct cgaactgcgc cgcgagcttc gccttgaacg cgggatcggc gtgcaggcgc
      661 gcgacctcgt actcgtcctt gtaggccatc agcttgaaca ggttgcgcgc gacggcctcg
      721 gtcaggcgcg tgcccttgga gaccttcgcc tcggccgcgc gcaccgcctc gacggcgctc
      781 gcgaagcgcg cggcgtaggc gccgtcctgg tagccggtga ggaactccac ccgcttggcg
      841 accacctcgg ccagcgacgg cctcttcacg aactcgatga cctggctggc atgagccggg
      901 gcgaagagcg cctgcaccga cgcgaggtcg tgcgcgcacc ggcggcccca ttcgaaggcc
      961 tgcttgttct tctccacctg gacgccgttg agctcgatcg atcgcatcag cgcggcgtgg
     1021 cccagcggga tgcggccctt ctgccacgcg tagcccagca tcagcgggtt ggtgaagatc
     1081 gagtcgccca gcagcttgac cgcgacctcg tcggcgtcga acgcgcccag cagctcgagg
     1141 ccgacggcct tggcgatcgc ggtctcgcag cggccgtgcg ggaactgcca gtcggggttg
     1201 ccgacgaagc cggcggtcgg cgagtcgtgc gtattgagcg cgacgtaggt gcggccttcg
     1261 cgcatcacct gcagcgtcgc cgggttcgcg gcgacgatgg cctcgcagcc gatcacgacg
     1321 tcggccttgg cggcgtcgac cttggtcgtc aggatcgcct cgggcgtgtc ggcgatctgg
     1381 atgtggctcc aggtcgcgcc gcccttctgc gcgaggcccg cggcgtcctg cgtgacgacg
     1441 cccttgccct cgaggtgcgc cgccatgccc agcagcgagc cgatcgtgat gacgcccgtg
     1501 ccgccgatgc cggcgacgac gatgccccag gcctgcggcg cggcggggat cgtgggcatc
     1561 ggcacctcgg gcagcgcggc cgcccgggac ttcggcgtcg ccggcttgcg cagctggccc
     1621 tcgacggtca cgaagctcgg gcagaagccc ttcacgcacg agaagtcctg gttgcaggtg
     1681 ttctggttga tgcggcgctt gcggccgaac tcggtctcca gcggctccac cgacaggcag
     1741 ttggactgca ccgagcaatc gccgcagccc tcgcacaccg cctcgttgat gacgacgcgg
     1801 cgcggctcgg gcgccatcgt gccgcgcttg cggcggcggc gcttctcggt ggcgcaggtc
     1861 tggtcgtaga cgatcacgct gacgccggcg acctcgcgca gctcgcgctg gatcgcgtcg
     1921 agctggtcgc ggtggtgcac cgtcgtgccc ggcgcgaggc ggattccggc gttgtacttc
     1981 tcgggcacgt cgctgacgac gacgatgcgc ttcacgccct cggcctcgag ctcgcgcgtg
     2041 atcgcgggga cgtcgatctg gccgtccacc ggctggccgc cggtcatcgc gacggcgccg
     2101 ttgaacagga tcttgtaggt gatgttcacg cccgcggcga gcgactggcg gatcgcgagc
     2161 aggcccgagt ggtagaacgt gccgtcgccg aggttggcga agatgtgcct gtcggtcgtg
     2221 aacggcgcct ggccgaccca gggcacgccc tcgccgccca tctgggtgaa cgtcgacgtc
     2281 gaccggtcca tccagttgac catgtagtgg cagccgatgc cggcgaccgc gcgcgagccc
     2341 tcgggcacgc gcgtcgaggt gttgtgcgga cagccggagc agaaccaggg cgtgcggtcg
     2401 cccacctgcg ccgaggcgtc ggacttcagc tccagcagcg cgcgatcctt ggcctcgatg
     2461 acggcgatgc gctcatccat gcggcgcgcg acgtcctcgg gcacgcccag cttcttcagg
     2521 cggcgcgcga cggccttggc gatgatcgcg ggcgtgaggt cggccttcgg acgcagcagc
     2581 cagttcgcgc ccgggttcgg cacggcccat tcgccgccgg agcggtcacc ctcggtctcg
     2641 tcgaacttgc ccagcacggt cggacgcacg tcgctgcgcc agttgtacag ctgctccttc
     2701 agctggtact cgatcatctg gcgcttctcc tcgacgacga ggatctcctt caggccctgc
     2761 gcgaagtcgc gcgcgatcgt ggcctccagc ggccagacga cgttgacctt gtggatgcgg
     2821 atgccgaggc ggcggcaggt cgcgtcgtcg aggcccaggt cggcgagcgc ctggcgcagg
     2881 tcgttgtagg ccttgccgct ggcgatcagg ccgaagcggt cgtcgggcgt ggagacgacg
     2941 ttgtggttca ggcggttcgc gcgcacgtag gcgagcgcgg cgtaccactt gtagtccatg
     3001 aggcgcgctt cctgctccag cggcgggtcg ggccagcgga tgtggacgcc gcccggcggc
     3061 atctcgaagt cctcgggcag gacgatcttc acgcgctgcg ggtcgacgtc gacgctggac
     3121 gccgattcga cgacctcctg gatcgtcttc acgcctgccc atacgccggc gaagcggctc
     3181 atcgcgaatg cgtgcaggcc catgtcgagg atgtcctgca ccgacgacgg gaagaacacc
     3241 ggcaggccgc aggccttgaa aatgtggtcg ctctggtggg cggcggtgga gctcttggag
     3301 atgtggtcgt cgcccgcgat cgcgatcgcg ccgccgtgct tcgccgtgcc ggccatgttg
     3361 gcgtgcttga acacgtcgca ggtgcggtcc acgcccgggc ccttgccgta ccagatgccg
     3421 aacaccccgt cgaacttctt cgactgcggg tagagatcga gctgctgcgt gccccagacg
     3481 gcggtggcgc cgagctcctc gttgacgccc ggctggaaga cgatgttctg cgccgcgagg
     3541 tgcttgcgcg cgctccacag cgcctggtcg tagccgccca gcggcgagcc gcggtagccg
     3601 ctgatgaagc cggcggtgtt caggccggcc agggcgtcgc gcgtgcgctg cagcatcggc
     3661 aggcgcacca gcgcctggac gccgctcatg aaggcgcggc cggtctcgag cgcgtacttg
     3721 tcgtcgagcg acaccgattc gagggccttg cggatgctgt cgggcaacgg ggcgttcatg
     3781 cgcacgtctc ctgcgggagg tgtcgaaaca agcaggccag tgtagggaaa aggggtcgac
     3841 aggtgcttgc tttttgcggg cgctgtgcga tgctccaaga aacgtccttg cttgacgaag
     3901 tcgattggag aaccagcgtt gccgagaagc gagaaacacg gaaaaacccg gcccgaaccg
     3961 acaccccgcg cggcggcgcc cgagccgccc ccgcccgcgc gcgagcagcg cgacgccggc
     4021 gcgctggatc gctacgacgt cgccctgctg caggagctcc agcgcgacgc ccgccagtcc
     4081 aacgcggatc tcgcggcgcg catcgggctg tcgcccgcgc cgacgtggcg ccgggtgcgc
     4141 cggctcgagg aactgggcgt catcaccggc taccgcgccg agatcgaccg gcgccgcatc
     4201 ggcctgggcg tgctcgcgtt cgtgcgcgtc gacgcggagc gggacaacgc cgacgcgacc
     4261 cgcgcgctgg aggacgccat ccgcgggctg ccggaggtca tctcctgcca ctacatcagc
     4321 ggcgccggca ccttcgagct gcaggtggtc gtgaccgatc tcgacgcgtt ctcgcgctgg
     4381 acgatgcaga ccctgttccg gctgccgaac gtgaaggacc tgcacaccag cttctcgctc
     4441 ggcgaggtca aggcgggggc ggcgctgccg ctcgggcacc tggccccggg gcgggggacg
     4501 gcatgacgct tgcaagaggc tccacgcgga ccaaggttcc cgccggcatc cctcgaacaa
     4561 gtccgttcgg gcgccgctta tcgactgcac gccgggcgcg tcgcggatgc agcatgggac
     4621 gaccggccga ccgtccgcgc gacaagttgc aaggcgggcg ggacagccgg cacggcgcgg
     4681 ggctacgatg cgggccgtcc gcccactaca accaacacgg cagggtcgcc attgaatcga
     4741 cgtgaggtca tcggctggat gggcgcgggg ttcgcggcgg ccctggcgcc ggcgtccgcc
     4801 atggccgcgc ccgacgcgtt gctgacgtcc gaggaacagg cctggctcgc cgaccacccg
     4861 gtggtgctgt acgcgccgga gcgcgacttc ccgccgttca gcttcgtcga cagccagggc
     4921 cagcaccgcg ggctgtcggc ggacgtcctc gacctcgtcg ccgcccacac cggcctgaag
     4981 ttccaggccg tcgccgcggg cgaccgcgcg accaacctcg agcgcctgcg ccgacgcgag
     5041 gtcgacctgc tcacctcgct gcgcccgacc accgagcgcg agggctacat cggtttcacc
     5101 agcgcctacg tcagctcgcc ggcggtgatc ctgcgccgcc gcggcgacca tcgccccggc
     5161 gacctcgcgc ggatggccgg cgagcacgtg gccgtcgacc gcaactccgc caccgagaac
     5221 ttcctgcgcg aacaggcgcc gcaggtggtg ccggtggagg tcgacgtcgc ggcgcaggga
     5281 ttgcgcgacc tcgtgttcgg cgaggtcgac gcggcggtct cgaccctcgc gaccgcgtcc
     5341 ttcatcatcg agcgcgaccg gctcggcggc atccgcgtgg cgggggagac gggctacttc
     5401 tcgacgctga cgatcggcta ccgcaaggac tggccgatgc tcggccgcat cctcgagaag
     5461 ggcctggcgc agatcaccga gtccgagcgc gccgggatca tcgcgcgctg gatcccgctg
     5521 tcggacatcg cgtggtggca gcggcccgag gtgcagcgcg tgctgggcgc ggcctcgatc
     5581 ggcaccggcc tgctgatggg cggcctgctg ctgtggaacc gggcgctgcg gcgcgccgtc
     5641 gcccagcgca ccgaggcgct gcagaaggag ctcgccgagc gccagcgcct ggagacgcgc
     5701 ctgcgcacgc tcgccgagca cgacccgctc accggcctga tgaaccgcgc ggcgctgacc
     5761 gaggcgttgc ggcgctcgct ggcgctggcg gcgcggcaga agtggtcggt ggcggtggtc
     5821 ttcgtcgacc tggacaagtt caaggccgtc aacgacaccc tgggccacgc cgccggcgac
     5881 gagctgctgc gccagatcgc ctcgcgcctg cagggctgcc tgcgcgagag cgacctgctg
     5941 ggccgcctgg gcggcgacga gttcatcgtc gtcgccgagg cgctgcacga cggcccgcgc
     6001 aacgcgatcg agctgaccga caagctgctc acgcagatga agcgtccatt cacgatcgag
     6061 gggcggccga tcagcgccgg cttcagcgcc ggcatcgcga tctacccggg cgacggcgac
     6121 acgcccgagg cgctcgtggc gcatgccgat gccgcgatgt accaggccaa gcagcagggc
     6181 cgcttccgtg ccgcgctgta ccagtccggc acgccggcgg tcgccgacgc ggcgacgcac
     6241 gacggcccgg ccgtgcccga gccggcgccc ggcggccggg agtcggcatg agcccccgca
     6301 ggctgcg
//
LOCUS       DASVLN010000012         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_322911, whole genome shotgun sequence.
ACCESSION   DASVLN010000012 DASVLN010000000
VERSION     DASVLN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322911"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF453_00245"
     CDS             complement(<1..287)
                     /locus_tag="VF453_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686094.1"
                     /translation="MFKPSPRLRSRSSRHARVDHVAGFAETQFAHHEDDEDYSGFAPT
                     EMFEQDPLPPLRLPSEALREAAAMATARVAVTAGDDAARSWATSRWVEAQEA"
     gene            complement(544..2076)
                     /locus_tag="VF453_00250"
     CDS             complement(544..2076)
                     /locus_tag="VF453_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686095.1"
                     /translation="MTSPATTRLTPAAMTLAVFAPFGNDPVLSGYPDGATRDVEGHPI
                     VGHLREVAATGVDVLLLIDRVDDRTHLVRLPAGGEVVDVEDRGKLDMLSPRTLADFVA
                     LAHACDPRAQLVLTFEGHGAGFMPELDAAELARLKAEADETYEWREERGVQRPFHRTG
                     ARANQPVEHRGAPLPGGNPTTPGGSPTMPGGSPTMPAGHRVMSTWALGEGLRLSGAPR
                     VAVLHFNNCFNMAVEVLHTVAPYAEAATGYCNYNFFSAGQAYPAVFRRLAEAGGASAL
                     ELAKWFADENHRVLHAAGHEPTVAGTVALERMQAVAAGVDALSDALLEALRGAPAGGR
                     HAVVDAIRDAIVAAQQYDSNGDDELDAPDELTDLDSLAAEFSRADFGDARVRAAAKAL
                     HEALADIKQYGDTGSPWMAPGVHWDFASENLAMNIFLPDPLLDGVWDWRSQYYLSINP
                     DTTRPQPQRHVIDFLKETDWGDFLLEYHRDTPLVGVRTAKPPRAPVLHTRADPRRRRA
                     RG"
BASE COUNT          335 a          829 c          915 g          337 t
ORIGIN      
        1 gcctcctgcg cctcgaccca gcgcgaggtg gcccaggaac gcgcggcgtc atcgcccgcc
       61 gtgaccgcca cgcgcgccgt cgccatcgcc gcggcctcgc gcagcgcctc cgagggcagg
      121 cgcagcgggg gcagcgggtc ctgctcgaac atctcggtcg gagcgaaccc gctgtagtcc
      181 tcgtcgtcct cgtgatgcgc gaactgggtc tcggcgaacc cggcgacgtg gtcgacgcgg
      241 gcatggcggg aggagcggga acgaaggcgg ggggaaggct tgaacatggt ctctactccg
      301 atgctgattc gtcgcggatc gacgacgagg tcgtctcgat ccaggaagaa tttcatcgcg
      361 gcgcagttcc catctcaacg caggtttgag ctgtcaggcg ggcagcggcg agacgattgt
      421 cgatcgattg gcggggggcc ggacctcccc aaaactgggg cttgaagacg aaaaaccccg
      481 ccaggccggt ctgaacctgg cgggggcgac gccgcccgat acggcgcgtc tgaaccggga
      541 gggtcatccg cgggcgcgcc gccggcgcgg atcggcccgc gtgtgcagca cgggcgcgcg
      601 cggcggcttc gcggtgcgca cgccgacgag gggcgtgtcg cggtggtact ccagcaggaa
      661 gtcaccccag tcggtctcct tcaggaagtc gatcacgtgg cgctgcggct gcgggcgcgt
      721 cgtgtcggga ttgatgctca ggtagtactg cgagcgccag tcccacacgc cgtcgagcag
      781 cggatcgggc aggaagatgt tcatcgccag gttctccgac gcgaagtccc agtgcacgcc
      841 gggcgccatc cagggcgagc cggtgtcgcc gtactgcttg atgtcggcga gcgcctcgtg
      901 cagcgccttg gccgcggcgc gcacgcgcgc gtcgccgaag tcggcccggg aaaactcggc
      961 ggccaggctg tcgaggtcgg tcagctcgtc cggcgcgtcc agctcgtcgt cgccgttgga
     1021 atcgtactgc tgcgccgcga cgatggcatc gcggatcgcg tcgacgaccg cgtggcgtcc
     1081 gccggccggc gcgccgcgca gcgcctccag cagggcgtcc gacaacgcgt cgacgccggc
     1141 ggcgacggcc tgcatgcgct cgagggcgac ggtgcccgcg acggtcggct cgtggccggc
     1201 ggcgtgcagg acgcgatggt tctcgtcggc gaaccacttg gcgagctcca gagcgctcgc
     1261 gccgccggcc tcggcgaggc gccggaagac cgcgggatac gcctggccgg ccgagaagaa
     1321 gttgtagttg cagtagccgg tcgccgcctc ggcgtacggc gccaccgtgt gcagcacctc
     1381 gacggccatg ttgaagcagt tgttgaagtg cagcacggcc acccgcggcg cgccggaaag
     1441 gcgcagcccc tcgccgagcg cccaggtcga catcacccgg tggcccgccg gcatcgtcgg
     1501 gctgccgccg ggcatcgtcg ggctgccgcc cggcgtggtc gggttgccgc cgggcagcgg
     1561 cgcgccgcga tgctccaccg gctggttcgc gcgcgcgccg gtgcggtgga acgggcgctg
     1621 cacgccgcgc tcctcgcgcc actcgtaggt ctcgtcggcc tcggccttga ggcgcgcgag
     1681 ctccgcggcg tcgagctcgg gcatgaagcc ggcgccgtgt ccctcgaaag tgagcacaag
     1741 ctgcgcccgc gggtcgcacg catgggctag cgccacgaag tcggcgagcg tgcgcggcga
     1801 gagcatgtcc agcttgccgc gatcctcgac gtcgacgacc tcgccgccgg cgggcaggcg
     1861 cacgaggtgc gtgcggtcgt ccacgcggtc gatcagcagc agcacgtcca cgccggtggc
     1921 ggccacctcg cgcaggtggc cgacgatcgg atggccctcc acgtcccgcg tggcgccgtc
     1981 ggggtagccg ctgaggacgg ggtcgttgcc gaacggcgcg aagacggcga gcgtcatcgc
     2041 ggcgggcgtg agtcgggtgg tcgcgggcga agtcatggga cgggtccctc ctgtcgggta
     2101 tgtacggccg atccccctcg tcggagggat ggcggtggac gcaaaatgcc gagcgataca
     2161 gtaacggacg cgttttcgca tcgcgacgca ggaatcgccc cgtccacgcg gccgggattg
     2221 gacgatcggt ggacgatgcc tcgaaagacg ccccgagccg agccgaggac gcatgcagcg
     2281 atcgaccctg atttccgcgg agaccgccga gaccctgctg gtcggcgcgc tcgcggccgt
     2341 gcgggcgggc agcctcggcg actggctgcg ccagcgtccc gtcgccgtgc gctggttcgc
     2401 gcgccgccac ctgcgg
//
LOCUS       DASVLN010000013         5735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_322954, whole genome shotgun sequence.
ACCESSION   DASVLN010000013 DASVLN010000000
VERSION     DASVLN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322954"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VF453_00255"
     CDS             complement(<1..864)
                     /locus_tag="VF453_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7686096.1"
                     /translation="MRYGGVEVLRAIDLRIAEGEFCVFVGPSGCGKSTLLRLVAGLED
                     ASGGEILMRGRRVDALPPAERDVAMVFQSYALYPHMTVRQNMSFGLEYRKVARAEIDR
                     RVQAASDMLQLGTLLDRKPRDLSGGQRQRVAIGRALVRQPAVFLFDEPLSNLDAALRV
                     QTRVEIARLHRSLGTAAMVYVTHDQVEAMTLADRIVLLRPQAGANGAPSIAQVGPPLE
                     LYHRPANRFVAGFIGSPAMNFLPAIAVGEAGEGLALRVAGIECVAHVDPRPITRAAPV
                     TAGIRPEHVRLG"
     gene            complement(906..2576)
                     /locus_tag="VF453_00260"
     CDS             complement(906..2576)
                     /locus_tag="VF453_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEX7686097.1"
                     /translation="MTASRADADWWRGAVIYQIYPRSFQDSNGDGIGDLAGITRRLPY
                     IAALGVDAIWISPFFPSPMRDFGYDISDYCGVDPRFGTLADLDALVAAAHAMGLKVLI
                     DLVLSHTASAHPWFAESRSSRDNAKADWYVWADARADGTPPNNWLSLFGGSAWQWDTE
                     RGQYYLHNFLASQPDLNYHCPEVRAAALDVVRFWLGRGIDGFRLDVVNFYYHDKELRS
                     NPPQPLGRFAAAVPPSNPYAFQSHLYDKTQPENLEFMQELRALLDTVPGTTTIGEIGD
                     DRQYHTLGEYTREGRLHMAYTFNLMGPDCTPRFIHGVLDEFLLETRDAWCCWALNNHD
                     VVRVTTRWRALGEPAALAPVLAAFMGSLRGSVCLYQGEELGLTEAEIAFEDVQDPYGK
                     EMWPRFKGRDGCRTPIPWSTREAHAGFSDAAPWLPMAPEHRAWAVEAQEAEPLSVLAR
                     YRRFLAFRRTQPALVRGAMRLLPAHDQVVAWVRAWMGDEVLCVFNLSSHPARYELAPC
                     DVAALEGHGFAGEVERDADGRAVACVLPPDGAYFGRLRAGDSFAEIQP"
     gene            complement(2627..3487)
                     /locus_tag="VF453_00265"
     CDS             complement(2627..3487)
                     /locus_tag="VF453_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7686098.1"
                     /translation="MTAVGNRRARSARAVAGRVGYVVAALIVMAVSVFPLLVAVSSSL
                     KTGDGLFDPALWPAAPTLENYRHLFAEHPFGRNLLNSLLVSSVVVALSLGLSLMAAWS
                     LGRVRFRGRGAMLMCILASSMFPQVAVLSGMFELVRALGLYDSLGALMVADLMLTLPF
                     TVWVLVTFMRQLPRELEEAAAMDGLGPLTLVFRIFLPLLWPALVATGLLAFIAAWNEF
                     LFALTFTLSPSSRTVPVAIALISASSAFELPWGGIMAASVIVTLPLVALVLLLQRHIV
                     SGLTQGAVKG"
     gene            complement(3484..4446)
                     /locus_tag="VF453_00270"
     CDS             complement(3484..4446)
                     /locus_tag="VF453_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7686099.1"
                     /translation="MSLALDRMPDAPAAPGAPPAPPRRGLAASRVRQALWMLAPMLVL
                     LAGVAGWPLARTVWISFTDMQLSDPQAARFVGLANYLGKYGLLTDPAWWSAVGITLAF
                     ALVSVTLEAALGLGVALLLDHPSRLRTALRACVLVPWAIPTVVSAKMWGWMLHDQFGV
                     VNHYLAALHLIHGPVAWTADPTLSLLSVVAVDVWKTTPFMALLILAALQTVPGDCYEA
                     AAVDGVPARAVFLRVTLPLIAPGVAVAVVFRLLDALRVFDLIYVMTANSRATRTMSVF
                     VREQLIDFQQVGYGSAAATVLFLVIGLSTLVTLRVLRSSAEDAQ"
     gene            complement(4466..>5735)
                     /locus_tag="VF453_00275"
     CDS             complement(4466..>5735)
                     /locus_tag="VF453_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010096307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7686100.1"
                     /translation="GGGRVLRCMLALAAAALAPAAMAASIKISCGAVGRELELCRESA
                     QAWAEKTGNQVQVVATPNDSSERLALYEQVLSSGSDKIDVFQVDVVWPGLLGSHFIDL
                     RPYAHGAEQGMFTDFVRADTKDGRLVAMPWFMNGGLLFYRRDLLAKYGLAAPQTWSEL
                     AADARRVQDAERAAGNDKMWGYVWQGRAYEGLTCDAVEWIASYGGGSIVEPDGRISID
                     NPRAAAALRNAASWVGTITPAAVLNYAEEESRGVFQAGNAVFMRNWPYAWSLAQAPQS
                     GIRGKVGVTVLPRTSAGPHVATLGGESLAVSKYSRNPAIAADLVMYMTSAEVQKQRAI
                     AASFNPSILALYRDPDVLAANPFMGELYPVFTHAVARPAVVAGTRYNQVSNEFWNSTH
                     EVLSGREKPEEALAELAATLTRVSRGGKWN"
BASE COUNT          886 a         2004 c         2132 g          713 t
ORIGIN      
        1 gcccaggcgg acgtgctcgg gccggatgcc cgcggtgacg ggcgcggcgc gcgtgatcgg
       61 gcgcgggtcg acatgcgcga cgcactcgat gccggccacc cgcagcgcca ggccctcgcc
      121 ggcctcgccg accgcgatcg cgggcaggaa gttcatcgcc ggcgagccga tgaagccggc
      181 cacgaagcgg ttggcgggac ggtggtacag ctccaggggc gggccgacct gcgcgatgct
      241 gggcgcgccg ttcgcgccgg cctgcggccg cagcagcacg atgcggtcgg ccagcgtcat
      301 cgcctcgacc tggtcgtgcg tcacgtagac catcgccgcc gtgccgagcg agcggtgcag
      361 gcgggcgatc tccacgcgcg tctgcacgcg cagcgccgcg tcgaggttgg acagcggctc
      421 gtcgaacagg aagaccgcgg gctggcgcac cagcgcgcgc ccgatcgcca cgcgctggcg
      481 ctggccgccc gagaggtcgc gcggcttgcg atccagcagc gtgccgagct gcagcatgtc
      541 gctcgccgcc tgcacgcgcc ggtcgatctc cgcgcgggcg accttgcggt actcgaggcc
      601 gaaggacatg ttctgccgca cggtcatgtg cgggtagagc gcgtagctct ggaacaccat
      661 cgcgacgtcg cgctcggccg gcggcagcgc gtcgacgcgg cggccgcgca tgaggatctc
      721 gccgccgctc gcgtcctcga ggccggcgac caggcgcagc agcgtcgact tgccgcagcc
      781 ggagggcccg acgaagacgc agaactcgcc ctcggcgatg cgcaggtcga tcgcgcgcag
      841 cacctcgacg ccgccgtagc gcatgtggac gccgcgcagc tcgatggcgg gcgtgtcgtt
      901 cgtggtcatg gctggatctc cgcgaatgaa tcgccggcgc gcagccggcc gaaataggcg
      961 ccgtcgggcg gcagcacgca ggcgacggcg cggccgtcgg catcgcgctc gacctcgccg
     1021 gcgaagccgt ggccctcgag ggcggcgacg tcgcacggcg cgagctcgta gcgcgccgga
     1081 tgcgaggaca ggttgaagac gcacagcacc tcgtcgccca tccacgcacg cacccaggcg
     1141 acgacctggt cgtgcgccgg cagcagccgc atcgcgccgc gcaccagcgc gggctgcgtg
     1201 cgccggaacg cgaggaagcg gcgatagcgc gcgagcacgg acagcggctc ggcctcctgc
     1261 gcctccacgg cccaggcacg atgctccggc gccatcggca gccagggcgc ggcgtcgctg
     1321 aagccggcgt gggcctcgcg cgtcgaccat gggatcggcg tgcggcagcc gtcgcggccc
     1381 ttgaagcgcg gccacatctc cttgccgtag gggtcctgca cgtcctcgaa cgcgatctcg
     1441 gcctcggtca ggcccagctc ctcgccctgg tacaggcaca ccgagccgcg cagcgagccc
     1501 atgaaggccg ccagaaccgg cgcaagcgcg gccggctcgc cgagcgcgcg ccagcgcgtg
     1561 gtcacgcgca ccacgtcgtg gttgttgagc gcccagcagc accacgcgtc ccgggtctcg
     1621 agcaggaact cgtcgagcac gccgtggatg aagcgcggcg tgcagtcggg ccccatcagg
     1681 ttgaacgtgt aggccatgtg cagccgccct tcgcgcgtgt actcgcccag cgtgtggtac
     1741 tggcggtcgt cgccgatctc gccgatcgtc gtggtaccgg gcaccgtatc gagcagcgcg
     1801 cgcagctcct gcatgaactc gaggttctcg ggctgcgtct tgtcgtagag gtggctctgg
     1861 aacgcgtagg gattgctggg cggcaccgcc gcggcgaagc gtccgagcgg ctgcggcggg
     1921 ttcgagcgca gctccttgtc gtggtagtag aagttgacga cgtcgagccg gaagccgtcg
     1981 atgccccgcc cgagccagaa ccgcaccacg tcgagcgcgg cggcgcgcac ctcggggcag
     2041 tggtagttga ggtcgggctg cgacgcgagg aagttgtgca ggtagtactg gccgcgctcg
     2101 gtgtcccact gccaggcgct gccgccgaac agggacagcc agttgttcgg cggcgtgccg
     2161 tcggcgcgcg cgtcggccca gacgtaccag tccgccttcg cgttgtcgcg gctcgagcgg
     2221 ctctcggcga accagggatg cgccgacgcg gtgtggctca gcacgaggtc gatcagcacc
     2281 ttcagcccca tggcgtgcgc cgccgcgacc agcgcgtcga ggtcggccag cgtgccgaag
     2341 cgggggtcga cgccgcagta gtcggagatg tcgtagccga agtcgcgcat cggcgacggg
     2401 aagaacggcg agatccagat cgcgtcgacg cccagggcgg cgatgtaggg cagccggcgc
     2461 gtgatgccgg cgaggtcgcc gatgccgtcg ccgttgctgt cctggaagct gcggggatag
     2521 atctggtaga tcacggcgcc gcgccaccag tcggcgtcgg cgcgggatgc ggtcatgttc
     2581 atcggggtgg tgcgaggacg ggggaaacgg gcgcgtgcgc ggggcgtcag cccttcacgg
     2641 cgccctgcgt gaggccggag acgatgtgcc gctgcagcag cagcacgagc gcgaccagcg
     2701 gcagcgtgac gatgaccgag gcggccatga tcccgcccca gggcagctcg aacgccgacg
     2761 aggcgctgat caacgcgatc gccaccggca ccgtgcgcga cgacggcgac agcgtgaacg
     2821 tgagcgcgaa caggaactcg ttccaggccg cgatgaacgc gagcagcccg gtggcgacga
     2881 gcgccggcca cagcagcggc aggaagatgc ggaacaccag cgtcagcggc ccgaggccgt
     2941 ccatcgccgc ggcttcctcg agctcgcgcg gcagctggcg catgaaggtc accagcaccc
     3001 agaccgtgaa cggcagcgtg agcatcaggt cggcgaccat cagcgcgccc aggctgtcgt
     3061 agaggcccag cgcgcgcacc agctcgaaca tgcccgacag caccgccacc tgcgggaaca
     3121 tcgacgacgc gaggatgcac atcagcatcg cgccgcgccc gcggaagcgc acgcggccga
     3181 gcgaccacgc ggccatgagg ctcaggccga ggctcagcgc gacgaccacg ctcgagacga
     3241 gcagcgagtt cagcaggttg cgaccgaacg ggtgctcggc gaagaggtgg cgatagttct
     3301 cgagcgtcgg cgcggcgggc cacagcgcag gatcgaacag gccgtcgccg gtcttgagcg
     3361 agctcgagac cgcgaccagc agcgggaaca ccgagaccgc catcacgatg agcgcggcga
     3421 cgacgtagcc gacgcgccct gcgacggcgc gcgcggagcg ggcgcgacgg ttgccgacgg
     3481 cggtcattgc gcgtcctcgg cagacgagcg cagcacgcgc agcgtgacga gcgtgctgag
     3541 cccgatgacg aggaacagca ccgtggccgc ggccgagccg tagccgacct gctggaagtc
     3601 gatcagctgc tcgcgcacga acacggacat cgtgcgcgtg gcgcggctgt tggcggtcat
     3661 cacgtagatc aggtcgaaca cgcgcagcgc gtccagcagc cggaagacga ccgccacggc
     3721 cacgcccggc gcgatcagcg gcagcgtgac gcggaggaag acggcgcgcg ccggcacgcc
     3781 gtcgacggcc gcggcctcgt agcagtcgcc gggcacggtc tgcagcgccg cgaggatcag
     3841 cagcgccatg aacggcgtcg tcttccagac gtcgacggcc acgaccgaca gcagcgacag
     3901 cgtcgggtcg gccgtccacg cgacgggccc gtggatcagg tgcagggccg cgaggtagtg
     3961 gttcacgacg ccgaactggt cgtgcagcat ccagccccac atcttggccg atacaacagt
     4021 gggtatcgcc cacggcacca gcacgcaggc gcgcagcgcg gtgcgcaggc gcgaaggatg
     4081 atcgagcagc agcgccacgc ccaggcccag cgcggcctcc agcgtcaccg acacgagcgc
     4141 gaaggcgagc gtgatgccca ccgcgctcca ccaggccgga tcggtcagca ggccgtactt
     4201 gccgaggtag ttggcgaggc cgacgaagcg cgcggcctgc gggtcggaca gctgcatgtc
     4261 ggtgaagctg atccacaccg tgcgggcgag cggccagccg gcgacgccgg ccagcagcac
     4321 gagcatcggc gcgagcatcc agagggcctg gcgcacgcgg ctggcggcga ggccacggcg
     4381 cggcggagcg ggcggcgcgc cgggcgcggc cggcgcgtcg ggcatgcggt cgagggcgag
     4441 gctcacggtg cgggctcctc gcggctcagt tccacttgcc gccgcggctg acgcgggtca
     4501 gggtggcggc gagctcggca agcgcctcct cgggcttctc gcggccggac agcacctcgt
     4561 gcgtgctgtt ccagaactcg ttgctcacct ggttgtagcg cgtgcccgcg accaccgctg
     4621 gccgcgccac cgcgtgcgtg aacaccgggt agagctcgcc catgaacggg ttggcggcga
     4681 gcacgtccgg gtcgcggtac agcgcgagga tgctcgggtt gaacgacgcc gcgatcgcgc
     4741 gctgcttctg cacttcggcg ctggtcatgt acatcacgag gtcggccgcg atggccgggt
     4801 tgcgcgagta cttcgacacc gccagcgatt cgccgccgag cgtcgccacg tgcgggccgg
     4861 cagaggtgcg cggcagcacc gtcacgccga ccttgccgcg gatgccgctc tgcggcgcct
     4921 gcgcgagcga ccacgcgtac ggccagttgc gcatgaagac ggcgttgccg gcctggaaca
     4981 cgccgcgcga ctcctcctcg gcatagttca gcaccgccgc gggcgtgatg gtgccgaccc
     5041 agctcgccgc gttgcgcagc gccgcggccg cgcgcgggtt gtcgatcgag atgcggccgt
     5101 cgggctcgac gatcgagccg ccgccgtagc tcgcgatcca ctccaccgcg tcgcaggtca
     5161 ggccctcgta ggcgcggccc tgccagacgt agccccacat cttgtcgttg ccggcggcgc
     5221 gctcggcgtc ctgcacgcgg cgggcgtcgg ccgcgagctc gctccacgtc tgcggcgccg
     5281 cgaggccgta cttggcgagc aggtccctcc gatagaacag cagcccgccg ttcatgaacc
     5341 agggcatcgc gacgaggcgg ccgtccttgg tgtctgcgcg cacgaagtcg gtgaacatgc
     5401 cctgctccgc accgtgcgcg tagggccgca ggtcgatgaa gtgcgagccg agcaggcccg
     5461 gccagacgac gtcgacctgg aacacgtcga tcttgtcgga gccggagctg agcacctgct
     5521 cgtagagcgc gaggcgctcg ctggaatcgt tgggggtggc caccacctgc acctggttgc
     5581 cggtcttctc ggcccaggcc tgcgccgact cgcggcacag ctcgagctcg cggcccacgg
     5641 cgccgcacga gatcttgatc gacgcagcca tggccgcagg cgcgagggcg gcggccgcca
     5701 gggcgagcat gcagcgcagg acgcgaccgc cgccg
//
LOCUS       DASVLN010000014         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_215618, whole genome shotgun sequence.
ACCESSION   DASVLN010000014 DASVLN010000000
VERSION     DASVLN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215618"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /gene="mdoH"
                     /locus_tag="VF453_00280"
     CDS             complement(<1..1879)
                     /gene="mdoH"
                     /locus_tag="VF453_00280"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX7686101.1"
                     /translation="MDQRHPSPTTSPRAGTMPAIARTPMLPRPWAGFRNSILGRGQSE
                     APRAPSAAAPWEAAAQRRRRMLLALIASATAFAAIVLAHAQPVHDHPVLQGAQIGLFS
                     LLFAWVAAGCFTALMGFMVQLRGGDRLAISARDAGDAPIPPDARTALIMPICHEDVAT
                     VFAGLRATAESLARAGALHAFDLFVLSDSKDPAVIAEERAAFDDLRATLGERGNVFYR
                     VRRVRTRRKAGNVADFCRRWGSNYRYMVVMDADSVMSGDCLQTLVRLMEKHPTAGILQ
                     TAPQAVGHATLHARAQQFAARVTGSLFTAGMQYWQLGESHYFGHNAIIRVAPFMQHCA
                     LAKLEGTGALAGEILSHDFVEAALMRRAGYHVWMVGDLVGSYEQQPPHLLAELQRDRR
                     WCQGNLQNARLIAEPGLHAVHRGMLATGAMSYVSAPLWLAYVVLGAALWLVGGNELFT
                     PEGTLAIGIPGLWAGTLTMLLLPRVLGVLAVILRREAHTHGGAFKLVQSAALEFLLAM
                     MLAPLRMVAHTLFVVGALTGWKLEWKSPPREANDVPWSQAAHRFGVVSLGVLALGAGM
                     SLAGESAVLWMLPVALPLVLAVPFTVLTGSTRLGERVRRARLLLVAEECDSPTVLRQA
                     WR"
BASE COUNT          289 a          689 c          716 g          224 t
ORIGIN      
        1 agcgccaggc ctggcgcagc acggtcggcg aatcgcactc ctcggccacc agcagcaggc
       61 gcgcgcggcg cacgcgctcg ccgagccgcg tgctgcccgt cagcaccgtg aacggcaccg
      121 ccagcacgag cggcagggcc accggcagca tccacagcac cgccgactcg ccggccaggc
      181 tcatgccggc gcccagcgcg agcacgccca ggctgacgac gccgaagcgg tgcgcggcct
      241 gcgaccaggg gacgtcgttg gcctcgcgcg ggggcgactt ccattccagc ttccagccgg
      301 tgagcgcacc cacgacgaac agcgtgtgcg cgaccatgcg cagcggcgcg agcatcatcg
      361 cgagcaggaa ctcgagcgcc gcgctctgca cgagcttgaa cgcgccgccg tgggtgtgcg
      421 cctcgcggcg caggatcacc gccagcacgc ccagcacgcg cggcagcagc agcatcgtca
      481 gcgtgccggc ccacaggccc gggatgccga tcgcgagcgt gccttcgggc gtgaacagct
      541 cgttgccgcc gaccagccac agcgccgcgc cgagcacgac gtacgcgagc cacagcggcg
      601 ccgagacgta ggacatcgcg cccgtggcga gcatgccgcg atgcaccgcg tgcaggccgg
      661 gctccgcgat caggcgcgca ttctgcaggt tgccctggca ccagcggcgg tcgcgctgga
      721 gctcggccag caggtgcggc ggctgctgct cgtagctgcc gacgaggtcg cccaccatcc
      781 agacgtggta gccggcgcgg cgcatcagcg cggcctcgac gaagtcgtgc gagaggatct
      841 cgccggccag cgcgcccgtg ccctcgagct tcgcgagcgc gcagtgctgc atgaagggcg
      901 cgacgcggat gatcgcgttg tggccgaagt agtgcgactc gcccagctgc cagtactgca
      961 tgccggcggt gaacaggctg cccgtcacgc gtgccgcgaa ctgctgggcg cgggcgtgca
     1021 gcgtcgcgtg gccgacggcc tgcggcgcgg tctgcaggat gccggcggtc gggtgcttct
     1081 ccatcaggcg caccagcgtc tgcaggcagt cgccgctcat cacgctgtcg gcgtccatca
     1141 cgaccatgta gcgatagttg gagccccagc ggcggcagaa gtcggcgacg ttgccggcct
     1201 tgcgacgggt gcgcacgcgg cgcacgcggt agaagacgtt gccgcgctcg cccagggtcg
     1261 cgcgcaggtc gtcgaaggcg gcgcgctcct cggcgatgac ggccgggtcc ttgctgtcgg
     1321 acagcacgaa caggtcgaac gcgtgcagcg cgccggcacg cgcgagcgac tcggcggtcg
     1381 cccgcaggcc ggcgaagacg gtggcgacgt cctcgtgaca gatcggcatg atcagcgccg
     1441 tgcgggcatc cggggggatc ggcgcatcgc cggcgtcccg cgccgagatc gcgagacggt
     1501 cgccgccgcg cagctgcacc atgaagccca tcagcgcggt gaagcagccg gccgcgaccc
     1561 acgcgaacag cagcgagaac aggccgatct gcgcgccctg cagcaccggg tggtcgtgca
     1621 ccggctgggc atgcgcgagc acgatcgccg cgaacgccgt cgccgacgcg atcagcgcca
     1681 gcagcatgcg acggcggcgc tgggccgcgg cctcccacgg cgctgccgcc gacggcgcgc
     1741 gcggggcctc tgactggccg cggcccagga tgctgttgcg gaacccggcc cacggacgcg
     1801 gcagcatggg cgtgcgcgcg atggccggca tcgtgccggc acggggcgaa gtcgtcgggg
     1861 aagggtgacg ttggtccatg tgtcgctcaa aaggtcgtcg ccggacggcg ggagcgtg
//
LOCUS       DASVLN010000015        16406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_376930, whole genome shotgun sequence.
ACCESSION   DASVLN010000015 DASVLN010000000
VERSION     DASVLN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16406
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376930"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VF453_00285"
     CDS             <1..665
                     /locus_tag="VF453_00285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7686102.1"
                     /translation="YNQPDAVARHVASWKALDAEVRRQLEFIVVDDYSDMPLAVPDRG
                     DLNLRLFRVTDDIDWNMPGCKNLAALMARADFLLFFDCDNIAPPEGLAALARAAGQLR
                     PDVLYNFRRVLEDGSEMPPHINTLLMSKRGFFQAGGMDEDFSGHYGFEDVHFHHMWRH
                     HVGSELLLADIAFTQLGYRTSTMNRDTSRNQALIHEKINVQQYRRSTGKLRFNWSEQL
                     AA"
     gene            complement(757..2961)
                     /locus_tag="VF453_00290"
     CDS             complement(757..2961)
                     /locus_tag="VF453_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7686103.1"
                     /translation="MQSNRFRARRPHQGLVLALGLAAAALASAQAPSHPPVAPVRPVV
                     DDYFGTKVTDDYRYLEDLGNPEVKAWMKGQADYTRARLDAIPGRAKLLERIHALLNTD
                     LRRAGFVQRGDRFFYEVIEPGAPLPKLYWRDGIKGEEHLLVDPGKLGEGSGTHYALDF
                     YEPSWDGKRIAYGVSAGGSEKSVMHVMDVATGKELGEAIDRTSDSVVAWRPDNKSFFY
                     LRYVKPTPGMPANQTMYNGRTYLHKLGAHLDGEGDAVVFGRGVNPALDVPEGQGTYVI
                     LSPDSSYAVAVANHNMDNNPSTVYVAPLAKVTDARTPWKKIADVADGATAFLPHGQTL
                     YVLSQKGAPRFRLLATPLAAPDLAKATVVIPEGSGVLTDVSLAQDGLYARTRDGAVSH
                     LHRVSYDGKRDELVPTPFEGNVAAATTDPKRPGALFGIRGWLQATRLLAYAPGAAASE
                     DTGLNPPASLDTSAFESREVFATSYDGTRIPLSLIYKKGLVADGSHPTILEGYGSYGV
                     SLEPGLNPTNVAWIERGGIIAVAHGRGGGEYGEGWHMAGYKKTKINTILDFIACGRWL
                     VDQKYTTAQRLAGEGGSAGGITVGGAMTWAPDAFGVILDLVGMSDTLRAETEPNGPPN
                     ISEFGSTHDEDGFHALYAMAAYEHIHDGVAYPAVIYSTGANDPRVAPWHMMKMAARTQ
                     AANPDGRPALLRIDYDAGHGIGSSMSQRESQLADLWSFALWQMGDPAFQPAH"
     gene            3165..3620
                     /locus_tag="VF453_00295"
     CDS             3165..3620
                     /locus_tag="VF453_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686104.1"
                     /translation="MRPRVPMCLAVLALAACAGTAAPPRDEAPDALTPDVLAKIGAHH
                     VPDLYRVIGTPAASEKTAHGTRYTWHAAVHETTFVPTAAPVAGFIGQLPTGAEGTGGG
                     GGEVGHDVTCRVRIDAGSTDLIEHLDFNGPRRACGPVTQRLAAWIRTVG"
     gene            complement(3648..4286)
                     /locus_tag="VF453_00300"
     CDS             complement(3648..4286)
                     /locus_tag="VF453_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686105.1"
                     /translation="MKPVLALAALLASFAGHAAPPDPTRDAQARAALAQAAPGVTFEP
                     DAAAFGDLDGDGVEDFAIVAGDPHGGPDGASAMRLFLFAGARSGGFRLAAKSAPLDDA
                     EVSIRRGSVFLHRDGASGCCAHWAEDFQFSLRGGQPMLIGLETAYAHPDDVQDADHGT
                     SVDLATGVVEHWTGRGGKPARRRRSSVRGLAPVPLAGFDDDAFHRRWRDALW"
     gene            complement(4347..4874)
                     /locus_tag="VF453_00305"
     CDS             complement(4347..4874)
                     /locus_tag="VF453_00305"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7686106.1"
                     /translation="MKLKHALVAATVAITALAATSANAAVTYTLDAQSSFPLDGETVT
                     GSYSFTVANFITSDTAIPLAALSNCSVTASLGPATCLGPDFLYHVNGADDQNEMAGLH
                     FDSAANPGSEAYYYFAPGSFEHYGTYDSVLFGASQQAVLTVSQASAVPEPSSPGFMAA
                     GLGLLALAARRARRR"
     gene            5032..5673
                     /locus_tag="VF453_00310"
     CDS             5032..5673
                     /locus_tag="VF453_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686107.1"
                     /translation="MAGNILIDPLDFMQKNLVVPFHDNLGTAQDQSGLIQMCLVKSQQ
                     PARRHGADVDKFILYRYRGQPNAEVAFRAFWCPYAQNEVRTCDLDDGANLMFTATMDG
                     CTFTATAQDAHGNCRVGHANSGRFGAAREATYGLAGARKFQSDEQSNRLRHALGAPAV
                     TISPADYMADHDGAMVQKSTTFAIRNGGQWRFYTQTYVIQGGRYFLRDVTQRA"
     gene            6018..6308
                     /locus_tag="VF453_00315"
     CDS             6018..6308
                     /locus_tag="VF453_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686108.1"
                     /translation="MVMKSLLAELVDGRLILPEEALAVLPAGAPLRVYVDTERGTVRI
                     HAKDPEAMPPDFEQFMDLLDELNEGVTLEEYTKPVPDSELRSGKPKNDEGKK"
     gene            6305..6874
                     /locus_tag="VF453_00320"
     CDS             6305..6874
                     /locus_tag="VF453_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7686109.1"
                     /translation="MSKQRAVLDANVLYGGFLRDLLLSVFAAGLYEAKWTEEITAEWV
                     RHLLKNRSNVTAESNARTVLRMHQIRPSALVENYKHLIESIDLPDKDDRHVVAAAIAC
                     QAQKIVTRNLRDFPSEILGAFGIVAQSPDSFLSELIRENAEGVVAAFRAMRKRFKKPP
                     MSVNDFFSALEKHELALTAKQLAHLRDLL"
     gene            7100..7600
                     /locus_tag="VF453_00325"
     CDS             7100..7600
                     /locus_tag="VF453_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686110.1"
                     /translation="MGIGMVVLALAIGNVWQYAVNRTDVSELQALAASQAATATAQAS
                     KTSITEQKRCAEIAAQAFARMGFDGVRHPLATFTNHYSRRFSRCYIDFQDTQVTGKSF
                     AVDRQIVDALENKSYGSYMWINGTDKKYWEVKPAECYVLTSTGIKQICTSDKEFEQLA
                     KTYMED"
     gene            7724..8122
                     /locus_tag="VF453_00330"
     CDS             7724..8122
                     /locus_tag="VF453_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7686111.1"
                     /translation="MREATSLLFKSFHEFVPLESLHTLPKRMRGIYALYCEDSPGDRN
                     LVYVGMTDSGALGRLVKHAEQKRERWTHCSVFEVWDNITLEQIKELEAWLLQMLRKDA
                     SANALNAQKGSKRFAALRRQTLARSRSNAS"
     gene            8362..8907
                     /locus_tag="VF453_00335"
     CDS             8362..8907
                     /locus_tag="VF453_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686112.1"
                     /translation="MKFDETDLELIKLVVTSVVAIIVGVVTAYIAWQQCQIARRQANI
                     AEQAKQVATAKLNFDLFEKRYRLYELLRNELLEIVICARRYDPSNSKYILEIPKFAFL
                     FGADITDYIGKIDDTHRALRSIWEREAKGVSEDERERSAEMLSWLNKQGIECYKRFKP
                     YLDFSEWQAQDWARTRVLPCD"
     gene            complement(9123..10235)
                     /gene="pheA"
                     /locus_tag="VF453_00340"
     CDS             complement(9123..10235)
                     /gene="pheA"
                     /locus_tag="VF453_00340"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX7686113.1"
                     /translation="MADTHDDAAPLPDLSTLRTQIDAADRELLEILNRRARLAQVVGE
                     VKKREGSVAFRPEREAQVIDGLKAANPGPLKTESVAPIWREIMSACRALETPTRVAYL
                     GPAGTFSEEAALGYFGSSIVRVPCPSVDEVFRVTTAGAADFGVVPVENSTEGMVARSL
                     DQFLVTPLFIIGETSLFVRHNLLRKTNSLEGISAVCAHPQALAQCHMWLNDHLPHAER
                     RPVASNAEGARLASLDPTLAAVASTRAGSEYGLHVVSPAIQDDANNRTRFAVITHTER
                     HPAPKASGHDCTSLVVTVSNRPGALHDMLVPLKAHGVSMTKFESRPARSGQWEYYFYV
                     DIAGHPDQEHVRTALEELRKVCGFFKILGTYPVDVH"
     gene            complement(10228..11334)
                     /gene="serC"
                     /locus_tag="VF453_00345"
     CDS             complement(10228..11334)
                     /gene="serC"
                     /locus_tag="VF453_00345"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX7686114.1"
                     /translation="MTMSERHRPWNFAPGPAAIPEAVLREAAAEMLDWHGCGVGVMEM
                     SHRGKEFMSIAAAAVADLRELLAVPAHFRILFLQGGGLGENAIVPMNLSRGGAVDFVQ
                     TGSWSTKSFNEAKRYCDAALAASNAGEALKAVPAPATWKLRAGASYVHVCSNETIDGV
                     EFQELPDLRALGSDVPLVIDCSSHILSRAIDWSRVGLAFAGAQKNIGPAGLTIVIVRE
                     DLLGHALPITPSAFDYRVVADNDSMFNTPPTWAIYIAGLTFKWIKAQGGVAAMEAAAV
                     AKAALLYGAIDASGLYASKVAPGSRSRMNVPFWLRDESLNDAFLAGAKERGLLALKGH
                     KSVGGMRASIYNAMPLAGVQALVDWLREFEKTHG"
     gene            complement(11331..14003)
                     /gene="gyrA"
                     /locus_tag="VF453_00350"
     CDS             complement(11331..14003)
                     /gene="gyrA"
                     /locus_tag="VF453_00350"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7686115.1"
                     /translation="MTQFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDARDGLKPV
                     HRRCLYAMHEQNNDWNRQHKKSARIVGDVMGKYHPHGDSAIYETIVRMAQPFSLRHML
                     VDGQGNFGSVDGDNAAAMRYTEIRLAKIAHEMLADIDKETVDFGPNYDGTEQEPLVLP
                     TRLPNLLVNGGTGIAVGMATNIPPHNLNEVVDGCLHLLRNPDATVEELMEIIPAPDFP
                     TGGIIYGISGVREGYRTGRGKVVMRAKVHFEPIDRGQRQAIVVDEIPYQVNKKTLLER
                     IAELVHEKKIEGISHIQDESDKSGMRVVIELKRGEVPEVVLNNLYKQTQLQDTFGMNM
                     VALIDGQPRLCNLKLLIAIFLEHRREVVTRRTIYELRKARERGHVLEGQAVALANIDE
                     FIKVIKESPTPPVAKTELMARSWDSPLVREMLARATADNPAGSAAFRPDGLPDHYGLQ
                     PDGLYKLSDTQAQEILQMRLQRLTGLEQDKVFGEYKDVMAEIADLLDILARKERVSTI
                     ISDELLALKSEFGTHKLAARRSLIEHNAQELGTEDLITPTDMVVTLSHTGYFKSQPLA
                     EYRAQRRGGRGKQATQTKDDDWVDQLFIANTHDWILCFSDLGRVYWLKVWEVPQGGRN
                     SRGKPIVNMFPLQKDEKITVVLPLTGEFRAFPADHFIFMATAQGTVKKTPLDDFSNPR
                     KAGIIAVNLDDGDHLIGAALTDGRHDVMLFSDGGKAVRFDEADVTPHGRASRGVKGMT
                     LEPGQRLIAMLVAEDETQSVLTATENGYGKRTSIVEYTRHGRGTKGMIAIQQSERNGK
                     VVAATLVRANDEIMLITDKGVLVRTRVSEVRELGRATQGVTLIALDDGAKLSGLQRIV
                     ENDAGEPSAIETSEGDEAPGGQGPAPSGDDGAAE"
     gene            14268..14936
                     /locus_tag="VF453_00355"
     CDS             14268..14936
                     /locus_tag="VF453_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7686116.1"
                     /translation="MKKLNTVAALFASVAMSTAFAQATTAPARVDNWRNVDGNVWKNG
                     TRELCWRDNFWTPATAVAECDGALKPVVEAPPAPPAPAPVAAAPAPAPVPAPVVAPVP
                     SSEKVTYAADAFFDFDKATLKPEAKEKLDDLVEKTKQINLEVIIAVGHTDSVGSDAYN
                     QKLSVRRAESIKTYLVSKGMEANRVYTEGKGEKQPVASNKTAEGRAKNRRVEIEVVGT
                     RRSN"
     gene            15068..15784
                     /gene="ubiG"
                     /locus_tag="VF453_00360"
     CDS             15068..15784
                     /gene="ubiG"
                     /locus_tag="VF453_00360"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX7686117.1"
                     /translation="MTQATNADPQELEKFGELAHKWWDTEGDFKPLHRINPLRLDWIA
                     QRAPLPGKAVLDVGCGGGILAESMAQRGAKVLGIDLSVKPLKVARLHAMEAGVAGLEY
                     REVAVEALAAERPASFDVVTCMEMLEHVPDPASVVASCAALARPGGRVFFSTLNRNAK
                     AFLFAIVGAEHVLKLLPKGTHEYAKFIRPSELSGFARAAGLEVTELAGMEYNPLTQRY
                     WLSGDTSVNYLVACRKPAAS"
     gene            15825..>16406
                     /gene="gph"
                     /locus_tag="VF453_00365"
     CDS             15825..>16406
                     /gene="gph"
                     /locus_tag="VF453_00365"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549795.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX7686118.1"
                     /translation="MSVAAIRGVLFDLDGTLVDSAPDLAGAANRLRAEHGLPPLPYED
                     LRPMVGSGARGMVGVAFGVRPGDSRFEQLRDAFLDRYETGLLERTRPFDGVEALLAAL
                     EAAGLPWGIVTNKATRFTRPIVEGLGLAARAAAVVCGDTTPHAKPHPAPLLHAAQAMG
                     VPPEGLAYVGDDLRDAQAARAAGMRMVAAAWGYL"
BASE COUNT         2615 a         5442 c         5660 g         2689 t
ORIGIN      
        1 tctacaacca gcccgatgcc gtggcgcgcc acgtcgcgtc ctggaaggcg ctggatgccg
       61 aggtgcggcg gcagctggag ttcatcgtcg tcgacgacta ctcggacatg ccgctggccg
      121 tgcccgaccg cggcgacctc aacctgcgcc tgttccgcgt caccgacgac atcgactgga
      181 acatgcccgg ctgcaagaac ctggccgcgc tgatggcgcg cgcggacttc ctgctgttct
      241 tcgactgcga caacatcgcc ccgcccgaag gcctcgcggc gctcgcgcgc gcggccgggc
      301 agctgcggcc ggacgtgctg tacaacttcc gccgcgtcct ggaggacggc tcggagatgc
      361 cgccccacat caacacgctg ctgatgtcca agcgcggctt cttccaggcc ggcggcatgg
      421 acgaggactt cagcggccat tacggcttcg aggacgtcca cttccaccac atgtggcggc
      481 atcacgtcgg ctcggagctg ctgctggccg acatcgcgtt cacgcagctg ggctaccgca
      541 cgtccacgat gaaccgcgac acgagccgca accaggcgct gatccacgag aagatcaacg
      601 tccagcagta ccgccggtcc acgggcaagc tgcgcttcaa ctggagcgag cagctggcgg
      661 cgtgacgaag aaagacccgt gacgagccgc cggcggcgcc ggggtcgtgc acgggtcgca
      721 gggcaggcct cgcgcccgcc gcttcgggct gccgcgtcag tgcgccggct ggaacgccgg
      781 atcgcccatc tgccacagcg cgaacgacca caggtcggcg agctgcgact cgcgctgcga
      841 catgctcgag ccgatgccgt ggccggcgtc gtagtcgatg cgcagcaggg cggggcggcc
      901 gtccgggttc gcggcctgcg tgcgcgcggc catcttcatc atgtgccagg gcgcgacgcg
      961 cgggtcgttg gcgccggtgc tgtagatcac cgcgggatag gccacgccgt cgtggatgtg
     1021 ctcgtaggcg gccatcgcgt acagcgcgtg gaagccgtcc tcgtcgtgcg tggagccgaa
     1081 ctccgagatg ttgggcgggc cgttgggctc ggtctcggcg cgcagggtgt cggacatgcc
     1141 cacgaggtcg aggatcacgc cgaacgcgtc cggcgcccag gtcatcgcgc cgccgacggt
     1201 gatgccgccc gcgctgccgc cttcgccggc caggcgctgc gccgtcgtgt acttctggtc
     1261 gaccagccag cggccgcagg cgatgaagtc gaggatcgtg ttgatcttcg tcttcttgta
     1321 gccggccatg tgccatccct cgccgtactc gccgccgccg cggccgtggg cgacggcgat
     1381 gatgccgccg cgctcgatcc aggcgacgtt ggtcgggttc aggccgggct ccagcgagac
     1441 gccgtagctg ccgtagccct ccaggatggt cggatggctg ccgtcggcga ccaggccctt
     1501 cttgtagatc agcgacaggg ggatgcgcgt gccgtcgtag ctcgtggcga agacctccct
     1561 ggactcgaag gccgaggtgt cgagcgaggc cggcgggttc aggcccgtgt cctcgctggc
     1621 ggccgcgccg ggcgcgtacg cgagcaggcg cgtggcctgc agccagccgc ggatgccgaa
     1681 cagcgcgccg gggcgcttcg gatcggtggt cgcggcggcg acgttgcctt cgaagggtgt
     1741 gggcacgagc tcgtcgcgct tgccgtcgta ggagacgcgg tgcaggtgcg agaccgcgcc
     1801 gtcgcgggtg cgcgcgtaca ggccgtcctg cgcgagcgag acgtcggtca gcacgccgga
     1861 gccctcgggg atgacgaccg tcgccttggc gaggtcgggc gcggccagcg gcgtcgcgag
     1921 caggcggaag cgcggcgcgc ccttctgcga cagcacgtac agcgtctggc cgtgcggcag
     1981 gaaggcggtc gcgccgtcgg cgacgtcggc gatcttcttc cacggcgtgc gggcgtcggt
     2041 gaccttggcg agcggcgcga cgtagaccgt ggacgggttg ttgtccatgt tgtggttcgc
     2101 gaccgcgacc gcgtaggacg agtccggcga caggatcacg taggtgccct ggccctcggg
     2161 cacgtccagc gccgggttga cgccgcggcc gaagacgacc gcgtcgccct cgccgtcgag
     2221 gtgcgcgccc agcttgtgca ggtaggtgcg gccgttgtac atcgtctggt tcgcgggcat
     2281 gcccggcgtc ggcttgacgt agcgcaggta gaagaaggac ttgttgtccg ggcgccaggc
     2341 gacgacgctg tcggaggtgc ggtcgatcgc ctcgccgagc tccttgccgg tggcgacgtc
     2401 catcacgtgc atcacgctct tctcggagcc gcccgccgac acgccgtagg cgatgcgctt
     2461 gccgtcccac gagggctcgt agaagtcgag cgcgtagtgc gtgccgctgc cttcgcccag
     2521 cttgcccggg tcgaccagca ggtgctcctc gcccttgatg ccgtcgcgcc agtacagctt
     2581 gggcagcggc gcccccggct cgatcacctc gtagaagaag cggtcgccgc gctgcacgaa
     2641 gccggcacgg cgcaggtcgg tgttcagcag cgcgtggatg cgctcgagca gcttggcgcg
     2701 gcccgggatc gcgtcgagac gcgcgcgcgt gtagtcggcc tggcccttca tccacgcctt
     2761 gacctcgggg ttgccgagat cctcgaggta gcggtagtcg tccgtgacct tggtgccgaa
     2821 gtagtcgtcg accaccgggc gcaccggcgc gacgggcgga tgcgaggggg cctgggcgga
     2881 cgcgagggcg gcggcggcca ggccgagcgc gaggacgagg ccctggtggg gccgccgcgc
     2941 gcggaagcgg ttggattgca tggggattgc ccttgtggaa tcggaaaagc gcgagtctat
     3001 gtgcaacgca ggctcgctca ggcccgcgat tcccccaggg cgcgggtcga gcccgcggcc
     3061 gggccgcatc atccgccgtc gagcatgctc ccccgcgcgc gaaccgagac acctgcggcg
     3121 ccggccggcc cagaatcgac gctccccggc aaggaggaac cgccatgcgc ccccgcgtcc
     3181 cgatgtgtct cgccgtcctc gcgctggccg cctgcgccgg cacggccgcg ccgcctcgcg
     3241 acgaagcccc cgacgcgctg acacccgacg tcctcgcgaa gatcggcgcc caccacgtgc
     3301 cggacctgta tcgcgtgatc gggacgcccg cggccagcga gaagaccgcg cacggcacgc
     3361 gctacacttg gcacgcggcc gtgcacgaga ccaccttcgt gcccaccgcg gcgccggtcg
     3421 ccggattcat cggccagctg cccacgggcg ccgagggcac gggcggcggc ggcggggagg
     3481 tcggccacga cgtgacctgc cgcgtgcgca tcgacgccgg cagcaccgac ctgatcgagc
     3541 acctcgactt caacggcccg cgtcgcgcgt gcgggccggt cacgcagcgg ctggcggcct
     3601 ggatccggac ggtcggctga ggagggatcc gtgcgctcgc cggtgcgtca ccacaacgcg
     3661 tcgcgccagc gccggtggaa cgcgtcgtcg tcgaagccgg ccaggggcac gggcgcgagg
     3721 ccacgcacgc tcgaacgcct gcgccgggcc ggcttgcccc cgcgtcccgt ccagtgctcg
     3781 acgacgccgg tggccaggtc gacgctcgtc ccgtggtcgg cgtcctggac gtcgtcgggg
     3841 tgggcgtagg cggtctccag gccgatcagc atcggctggc cgccgcgcaa gctgaactgg
     3901 aagtcctcgg cccaatgagc gcagcagccg ctcgcgccgt cacggtgcag gaagaccgag
     3961 ccgcgccgga tcgagacctc ggcgtcgtcc agcggcgccg acttcgccgc gagccggaag
     4021 ccgccggagc gggcgcccgc gaagaggaac aggcgcatcg cgctggcgcc gtcggggccg
     4081 ccgtgcggat cgccggccac gatcgcgaag tcctcgacgc cgtccccgtc caggtcgccg
     4141 aaggccgcgg cgtccggctc gaacgtcacg cccggagcgg cctgcgccag cgcggcgcgg
     4201 gcctgggcgt cgcgcgtcgg gtccggcggc gcggcatggc cggcgaacga ggcgaggagg
     4261 gccgcgagcg cgaggacggg cttcatgcgg tcatgagcgt cccgcgagcg tgcgccgacg
     4321 tgcgagcccg cagtgaggcg aaaacgtcag cggcgacgcg cacgccgcgc ggccagggcc
     4381 aggagtccga ggccggccgc catgaatccg ggcgacgacg gctcgggcac cgcgctcgcc
     4441 tgcgacaccg tcagcacggc ctgctgcgac gcgccgaaga gcacggagtc gtaggtgcca
     4501 tagtgctcga acgaaccggg cgcgaagtag tagtaggcct ccgagccggg attggccgcg
     4561 gaatcgaagt gcagtccggc catctcgttc tggtcgtccg cgccgttgac gtgatacagg
     4621 aaatccgggc cgaggcaggt cgccgggccg aggctggccg tcaccgagca gttgctcagc
     4681 gcggcaagcg ggatcgcggt gtcggaggtg atgaagttcg ccacggtgaa cgagtacgag
     4741 cccgtgaccg tctcgccatc gagcgggaag gacgactgcg cgtccagcgt gtaggtgacg
     4801 gcggcgttcg ccgaggtggc ggccagcgcg gtgatcgcca cggtcgcggc gaccagtgcg
     4861 tgcttcagct tcatcgattc ctcctggttg agcagcggcg cagcatagcg gtccgtcttg
     4921 gcgtgccggt tccccaaaac gggtgaggcg gtgagcgctc tcccgcgctg gtcgggcggc
     4981 ccgtttcaag cgatgatccc gatcctctca attcgccccc ggagtcaggc cttggctggc
     5041 aacatcctga tcgacccgct cgacttcatg cagaagaacc tggtcgtgcc gtttcacgac
     5101 aacctcggca ccgcacagga ccagtccggg ctgatccaga tgtgcctggt caagtcccag
     5161 cagccggcgc gtcgtcacgg cgcggacgtc gacaagttca tcctgtatcg ctatcgcggc
     5221 cagcccaatg ccgaggtggc cttccgtgcc ttctggtgcc cctacgcgca gaacgaggtg
     5281 cgcacctgcg acctggacga cggcgcgaac ctgatgttca ccgccacgat ggacggctgc
     5341 acgttcacgg ccaccgcgca ggacgcgcac ggcaactgcc gcgtcggcca tgcgaactcg
     5401 ggccggttcg gcgcggcccg cgaagccacc tacggcctcg ccggcgcgcg caagttccag
     5461 tccgacgagc agtcgaaccg cctgcgccac gcgttgggcg ccccggccgt caccatcagt
     5521 ccggccgact acatggccga tcacgacggc gccatggtgc agaagtcgac caccttcgcg
     5581 atccgcaacg gcgggcagtg gcgcttctat acccagacct acgtgatcca gggcgggcgc
     5641 tacttcctgc gggacgtcac ccagcgcgcc tgacggcgcc atgaaaaggc ggcacccgag
     5701 ggtgccgtcg cgccgcctgt cgcacgcggt tcagtgcacg tccaccggat acgtccccag
     5761 gatcttgaag aacccgcaca ccttgcgcag ctcctcgagc gccgtgcgca cgtgctgctg
     5821 gtcgcttggg acatcacccg aggtcgcggc gtcgcaactt cgtttgtcgc tgcccaggag
     5881 tcccctgaag gaggggatag cccgttgggc ggcccgtcct tagcatgcca gtcgtggctc
     5941 tggcatcgcc gcaccgctac cctaccatct tgccaggctg aacctgctac agatactcac
     6001 cacataggaa gaccgccatg gtcatgaaga gcttgctggc cgagttggtc gacggccgtt
     6061 tgatcctgcc cgaggaagcg ctcgccgtcc tgccggcagg cgccccgctg cgggtctacg
     6121 tcgacaccga gcgcggaaca gtgcgcatcc acgccaaaga cccggaagcc atgccgcccg
     6181 acttcgagca attcatggac ttgctcgatg aactcaacga aggcgtgacc ctggaggaat
     6241 acaccaagcc cgtcccggac agcgaacttc gcagcggcaa gccgaagaac gacgagggca
     6301 agaaatgagc aagcagcggg cggtactgga tgcgaacgtt ctgtatggcg ggtttctcag
     6361 ggatctgctg ctctccgtgt ttgcggcggg tctttatgaa gcgaagtgga ccgaagagat
     6421 cacggcagag tgggttcggc acctgctgaa gaatcggtct aacgtcacgg ccgaaagcaa
     6481 cgctcgtacg gtgcttcgga tgcatcagat ccggccgagt gcgcttgtgg agaactacaa
     6541 gcacttgatc gagagcatcg atcttccaga caaagacgac cgacatgtgg tggcggccgc
     6601 gattgcctgt caggcacaga agatcgtgac caggaatctg cgagatttcc cgagcgagat
     6661 actcggcgca ttcggcatcg tcgcgcagag tcccgacagc tttctgtcgg agctgatcag
     6721 ggagaatgcc gagggcgttg tggcggcctt tcgcgcgatg cgcaagcggt tcaagaagcc
     6781 gccgatgtcc gttaacgact ttttttcggc tcttgagaag catgaacttg cgctgaccgc
     6841 caaacaactc gcgcaccttc gcgatctgct gtgatcgagc ttgcggtgac gcgccatcac
     6901 gtcaccgcgc cgtgcaaggc cttcgagtcc ggagttatgc cacggcaccg accggcgccc
     6961 gggttgctgc cgtgggcggc acatgatttc cgccaaacgt catcccgtat caacaggcgc
     7021 gggaagcact gccgaatgcc tgccgttgag ctacgattct ccgagggagg acacgtctat
     7081 gactctaagc aagagcgccg tgggaatcgg catggtcgtt ttggcgctgg ccattggaaa
     7141 cgtctggcag tacgcggtaa atcgaacgga cgtatcggaa ctgcaagcac tcgcggcctc
     7201 ccaggcagca accgcgaccg cacaagcttc caagacgagt atcacagaac agaagcgatg
     7261 cgccgaaatc gctgcacagg cgttcgcgcg catgggcttc gacggtgttc gtcacccact
     7321 cgctacattc acgaatcact acagccgacg cttctcgcga tgctacattg acttccagga
     7381 tacgcaagtg accggaaagt ccttcgcggt tgatcgccag atcgttgacg ccctggagaa
     7441 taagtcgtac ggctcctaca tgtggatcaa cggcacggac aagaagtact gggaagtcaa
     7501 gcccgctgag tgctacgtct tgacgtctac tggaatcaag cagatctgca caagcgacaa
     7561 agagtttgaa cagcttgcaa aaacctacat ggaggactga tggccgtacc accaccagca
     7621 agctcgcaac taggcgccga cgaccaggca attgccgcac ctatagtcgc gtgggcgcag
     7681 cataatcgtc tgtttggcca acttcttgga tttagatgtt gacatgcgtg aagccacctc
     7741 attgcttttc aagtcattcc acgagtttgt gcctttggag agtctgcaca ccctgccaaa
     7801 gcggatgcgc ggcatctatg cgctctactg cgaagactca ccgggcgatc gaaatctggt
     7861 ttacgttgga atgactgatt ccggcgcttt aggcaggctc gttaaacatg ccgaacaaaa
     7921 gcgggaacgc tggacgcact gttctgtctt tgaagtatgg gacaacatca cactggagca
     7981 gatcaaagag cttgaggcgt ggctgctgca aatgctccgc aaagatgcct ccgcaaacgc
     8041 cctcaacgcg caaaaggggt ccaagaggtt cgctgctctg cgacggcaga ccttggcccg
     8101 atctaggtcg aatgcttcct gacttccggt caagcggcga actttggaac ttcaagctgg
     8161 aactcggact tgccgcgatc gtggctgtgc ctcgaaatca cgatctaaag cggttcgtcg
     8221 cacaacggaa cgtacaggcg atagctttcc gatcgtagtt cagcttggcg gttgctacgt
     8281 gctttggctg ctcagcgatg gccgcctgac gctgcgcgat ggtccgctgc cgccaagcta
     8341 catagacgga gaatcgtttg catgaaattc gacgaaactg acctcgaact tatcaagctg
     8401 gttgtcacat ctgtcgtggc catcatcgtc ggtgtggtca cggcgtacat agcatggcag
     8461 cagtgccaaa ttgccaggcg gcaagccaac atagcagagc aagccaaaca ggtcgcgaca
     8521 gcaaagttga actttgacct gtttgaaaag cgctataggc tctacgagct cctcagaaat
     8581 gaactactgg aaattgtcat ctgcgctcga agatacgatc cttctaattc taagtacatt
     8641 ctcgaaattc cgaagtttgc gttcctcttc ggagctgaca tcacggacta catcggaaag
     8701 attgacgaca cgcatcgtgc cttgagatcg atctgggagc gcgaagcgaa aggggtgtca
     8761 gaggatgagc gcgaaaggtc cgccgaaatg ctctcgtggt tgaacaaaca aggcatcgaa
     8821 tgctacaagc gcttcaagcc gtacttggac ttcagcgagt ggcaagccca ggattgggcg
     8881 agaacacggg ttttaccttg cgactgatca gtcctaatgc ggacaagtcc ggctgtgagg
     8941 tgcttcccgc cactggccag cggacttgct ggcatgggtg aggtgagctc acgcgatggg
     9001 tcaacgcttg ggagcaagat agccctgggc tgcacggaaa taccggcgag ttacccgtat
     9061 aggcggtatg aaaagcgacc tatggggccg tcaaaggtgc tttgacggtt gtagggatga
     9121 tctcaatgca cgtccaccgg atacgtcccc aggatcttga agaacccgca caccttgcgc
     9181 agctcctcga gcgccgtgcg cacgtgctcc tggtccgggt ggccggcgat gtcgacgtag
     9241 aagtagtact cccactggcc cgagcgggcc gggcgcgact cgaacttggt catcgagacg
     9301 ccgtgcgcct tcaggggcac gagcatgtcg tgcagggcgc ccgggcggtt ggagaccgtg
     9361 acgacgaggc tggtgcagtc gtggcccgag gccttgggcg cgggatggcg ctcggtgtgc
     9421 gtgatgacgg cgaagcgcgt gcggttgttc gcgtcgtcct ggatcgccgg cgagaccacg
     9481 tgcaggccgt actcgctgcc cgcgcgcgtg ctcgcgacgg ccgccagcgt cgggtcgagc
     9541 gacgcgagcc gcgcgccctc ggcgttgctc gcgacgggac ggcgctccgc gtggggcagg
     9601 tggtcgttga gccacatgtg gcattgcgcc agggcctgcg ggtgcgcgca gaccgccgag
     9661 atgccctcga gcgagttcgt cttgcgcagc aggttgtggc gcacgaacag gctggtctcg
     9721 ccgatgatga acagcggcgt gacgaggaac tggtccagcg agcgcgcgac catgccctcg
     9781 gtggagttct cgaccggcac gacgccgaag tccgccgcgc cggcggtcgt gacgcggaag
     9841 acctcgtcca cgctcgggca gggcacgcgc acgatggacg agccgaagta gccgagcgcg
     9901 gcctcctcgc tgaacgtgcc ggcggggccg aggtaggcga cgcgcgtggg cgtctccagc
     9961 gcgcggcagg ccgacatgat ctcgcgccag atcggcgcca cgctctcggt cttcagcggg
    10021 ccggggttgg cggccttcag gccgtcgatg acctgggctt cccgctcggg ccggaaggcg
    10081 accgagccct cgcgcttctt cacctcgccg acgacctgcg cgagcctcgc gcggcggttg
    10141 aggatctcga gcagctcgcg gtcggcggcg tcgatctgcg tgcgcagcgt cgacaggtcg
    10201 ggcagcggcg cggcgtcgtc gtgcgtgtca gccatgggtc ttctcgaact cgcgcagcca
    10261 gtcgacgagc gcctggaccc ccgccagcgg catcgcgttg tagatcgacg cgcgcatgcc
    10321 gccgacgctc ttgtggccct tgagcgccag cagcccgcgt tccttcgcgc cggcgaggaa
    10381 ggcgtcgttc agcgactcgt cgcgcagcca gaacggcacg ttcatgcgcg agcgcgagcc
    10441 cggggcgacc ttgctggcgt acaggccgct cgcgtcgatc gcgccgtaca gcagcgccgc
    10501 cttcgcgacc gccgcggcct ccatcgccgc gacgccgccc tgtgccttga tccacttgaa
    10561 ggtgaggccg gcgatgtaga tcgcccaggt cggaggcgtg ttgaacatcg agtcgttgtc
    10621 ggccaccacg cggtagtcga acgccgaggg cgtgatcggc agcgcgtggc ccagcaggtc
    10681 ctcgcgcacg atgacgatgg tcaggcccgc cgggccgatg ttcttctgcg cgcccgcgaa
    10741 ggcgaggccg acgcgcgacc agtcgatcgc gcgcgagagg atgtgcgacg agcagtcgat
    10801 caccagcggg acgtccgagc cgagcgcgcg caggtcgggc agctcctgga actcgacgcc
    10861 gtcgatggtc tcgttgctgc acacgtgcac gtacgaggcg ccggcgcgca gcttccatgt
    10921 cgccggcgcg ggcacggcct tgagcgcctc gcccgcgttg ctcgcggcca gcgcggcgtc
    10981 gcagtagcgc ttcgcctcgt tgaaggactt ggtcgaccag ctgcccgtct gcacgaagtc
    11041 gaccgcgccg ccgcgcgaga ggttcatcgg cacgatggcg ttctcgccca ggccgccgcc
    11101 ctgcaggaac aggatgcgga agtgggcggg cacggcgagc agctcgcgca ggtccgccac
    11161 ggcggcggcg gcgatggaca tgaactcctt gccgcggtgg ctcatctcca tcacgccgac
    11221 gccgcagccg tgccagtcca gcatctcggc ggcggcctcg cgcagcaccg cctcggggat
    11281 ggcggcgggg ccgggcgcga agttccaggg gcgatggcgc tcgctcatcg tcattccgcc
    11341 gcgccgtcgt cgccggacgg ggccgggccc tggccgcccg gggcctcgtc gccctcgctc
    11401 gtctcgatcg cgctcggctc gccggcgtcg ttctcgacga tgcgctgcag gcccgacagc
    11461 ttggcgccgt cgtccagcgc gatcagggtg acgccctgcg tcgcgcggcc cagctcgcgc
    11521 acttcgctca cgcgcgtgcg cacgaggacg cccttgtcgg tgatcagcat gatctcgtcg
    11581 ttggcgcgca ccagcgtcgc ggcgacgacc ttgccgttgc gctcgctctg ctggatcgcg
    11641 atcatgccct tggtgccgcg gccgtggcgc gtgtactcga cgatcgaggt gcgcttgccg
    11701 tagccgttct cggtggcggt gagcacgctc tgcgtctcgt cctcggccac cagcatcgcg
    11761 atgaggcgct ggcccggctc gagcgtcatg cccttgacgc cgcgcgaggc gcggccgtgg
    11821 ggcgtgacgt cggcctcgtc gaagcgcacc gccttgccgc cgtcgctgaa cagcatgacg
    11881 tcgtggcggc cgtcggtcag cgcggcgccg atcaggtggt cgccgtcgtc gaggttgacc
    11941 gcgatgatgc ccgccttgcg cgggttgctg aagtcgtcca gcggcgtctt cttgaccgtg
    12001 ccctgcgcgg tggccatgaa gatgaagtga tcggccggga aggcgcggaa ctcgcccgtc
    12061 agcggcagca cgacggtgat cttctcgtcc ttctgcagcg ggaacatgtt gacgatgggc
    12121 ttgccgcgcg agttgcgccc gccctgcggc acttcccaga ccttgagcca gtagacgcgg
    12181 ccgaggtcgc tgaagcacag gatccagtcg tgcgtgttgg cgatgaacag ctggtcgacc
    12241 cagtcgtcat ccttggtctg cgtcgcctgc ttgccgcggc cgccgcggcg ctgcgcgcgg
    12301 tactcggcga gcggctggct cttgaagtag ccggtatggc tcagcgtcac gaccatgtcg
    12361 gtcggcgtga tcaggtcctc ggtgccgagc tcctgcgcgt tgtgctcgat caggctgcgg
    12421 cgtgccgcga gcttgtgggt gccgaactcc gacttcagcg cgagcagctc gtcgctgatg
    12481 atggtcgaca cgcgctcctt gcgcgcgagg atgtcgagca ggtcggcgat ctcggccatc
    12541 acgtccttgt actcgccgaa gaccttgtcc tgctccaggc cggtcaggcg ctgcaggcgc
    12601 atctgcagga tttcctgcgc ctgcgtgtcg gacagcttgt acaggccgtc gggctgcagg
    12661 ccgtagtggt cgggcaggcc gtcggggcgg aacgcagcgc tgccggccgg gttgtccgcg
    12721 gtcgcgcgcg cgagcatctc gcgcaccagc ggcgaatccc agctgcgcgc catcagctcg
    12781 gtcttggcga ccggcggcgt cggcgattcc ttgatgacct tgatgaactc gtcgatgttg
    12841 gcgagcgcga cggcctggcc ctcgagcacg tggccgcgct cgcgcgcctt gcgcagctcg
    12901 tagatcgtgc ggcgggtcac gacctcgcgg cggtgctcaa ggaagatcgc gatcagcagc
    12961 ttgaggttgc acagtcgcgg ctggccgtcg atcagcgcga ccatgttcat gccgaacgtg
    13021 tcctgcagct gcgtctgctt gtacaggttg ttcagcacga cctcgggcac ttcgccgcgc
    13081 ttcaactcga tcacgacgcg catgcccgac ttgtcggact cgtcctggat gtggctgatg
    13141 ccctcgatct tcttctcgtg cacgagctcg gcgatgcgct cgagcagcgt cttcttgttg
    13201 acctggtacg ggatctcgtc gacgacgatg gcctggcgct ggccgcggtc gatcggctcg
    13261 aagtgcacct tggcgcgcat cacgaccttg ccgcggccgg tgcggtagcc ctcgcgcacg
    13321 ccggagatgc cgtagatgat gccgccggtg gggaagtcgg gcgccgggat gatctccatc
    13381 agctcctcga ccgtggcgtc cgggttgcgc agcaggtgca ggcaaccgtc gacgacctcg
    13441 ttgaggttgt gcggcgggat gttggtggcc atgcccaccg cgatgccggt gccgccgttg
    13501 accagcaggt tgggcaggcg cgtcggcagc accagcggct cctgctcggt gccgtcgtag
    13561 ttggggccga agtcgacggt ctccttgtcg atgtcggcca gcatctcgtg cgcgatcttg
    13621 gccagccgga tctcggtgta gcgcatggcc gcggcgttgt cgccgtcgac ggagccgaag
    13681 ttgccctggc cgtcgaccag catgtgccgc agcgagaacg gctgcgccat gcgcacgatg
    13741 gtctcgtaga tcgcggagtc gccgtgcggg tggtacttac ccatcacgtc gccgacgatg
    13801 cgcgccgact tcttgtgctg gcggttccag tcgttgttct gctcgtgcat cgcgtacagg
    13861 cagcggcgat ggacgggctt caggccgtcg cgggcgtcgg gcagggcccg gcccacgatg
    13921 acgctcatcg cgtagtccag gtacgagcgc cgcatctcct cttcgaggct gacgggcagg
    13981 gtttccttgg caaattgggt catggcctac agacaggagt cctggcttcc gggtggatcc
    14041 cggagcaggc tgaatccaag gattctaagg gctgcgggcc tgtcgcggtc tcgcaacact
    14101 gacggggacg aatcgagcgc ggtcgtgcaa aaacaggctt ccgtcacggg ccgatggcag
    14161 aatgattttt gtcccttggt ggccggcctg tgcggtggcg cgccaacgtt tgcatccgat
    14221 ttggacgttt cgcaggcaac tgcggcccta tccttgagag gagaatcatg aagaaattga
    14281 acacggtggc ggcgctgttc gcgtcggtcg ccatgtcgac cgcctttgcc caggccacga
    14341 cggctcccgc ccgcgtcgac aactggcgca acgtcgacgg caacgtctgg aagaacggta
    14401 cccgcgagct gtgctggcgc gacaatttct ggacccccgc gaccgcggtg gccgagtgcg
    14461 acggcgccct gaagccggtc gtcgaagccc cgcccgcgcc gccggccccc gccccggtgg
    14521 ccgccgcccc ggccccggca ccggtccctg ccccggtcgt cgcgccggtg ccgtcgagcg
    14581 agaaggtcac ctacgccgcc gacgcgttct tcgacttcga caaggcgacg ctgaagccgg
    14641 aagccaagga aaagctcgac gacctcgtcg agaagaccaa gcagatcaac ctggaagtca
    14701 tcatcgccgt cggccacacc gacagcgtcg gctcggacgc ctacaaccag aagctgtcgg
    14761 tccgccgcgc cgagtccatc aagacctacc tggtctcgaa gggcatggaa gccaaccgcg
    14821 tctacacgga aggcaagggc gagaagcagc ccgtggcgag caacaagacc gccgaaggcc
    14881 gcgcgaagaa ccgtcgcgtg gaaatcgaag tggtcggcac ccgccgctcg aactgatcgg
    14941 gacgccgcgc ggggcatcgc ctcgcgccgt tcctccggaa acccgcctcg gcgggttttt
    15001 tcatttccgg ccgggggttt tccgcgcttg ctggaaaccc tgccgacacg cgctagcatt
    15061 cgtccccatg acccaagcca ccaacgccga tccgcaggaa ctcgagaaat tcggtgaact
    15121 cgcccacaag tggtgggata ccgaaggcga cttcaagcca ctgcaccgca tcaacccgct
    15181 gcggctcgac tggatcgccc agcgcgcgcc gctgccgggc aaggcggtgc tggacgtggg
    15241 ctgcggcggc ggcatcctgg ccgagtccat ggcgcagcgg ggcgcgaagg tgctgggcat
    15301 cgacctctcg gtcaagccgc tgaaggtcgc ccggctgcac gcgatggagg cgggcgtcgc
    15361 cgggctcgag taccgcgagg tcgccgtcga ggcgctggcg gccgagcggc cggccagctt
    15421 cgatgtcgtc acctgcatgg agatgctcga gcacgtgccc gacccggcgt cggtggtcgc
    15481 gagctgcgcg gcgctggcca ggcccggcgg ccgggtgttc ttcagcacgc tcaaccgcaa
    15541 cgccaaggcc ttcctcttcg cgatcgtcgg cgcggagcac gtgctcaagc tcttgcccaa
    15601 gggcacgcac gaatacgcga agttcatccg cccgagcgag ctctcgggct tcgcgcgggc
    15661 ggcgggcctg gaggtcacgg agctcgccgg catggaatac aacccgctga cgcagcgcta
    15721 ctggctctcg ggcgatacct cggtgaacta cctcgtggct tgccgcaagc cggcggcgtc
    15781 gtgagcgccc gcccggccgc agtgcgccgc acggaggacg tctcgtgagc gtcgccgcca
    15841 tccggggcgt gctgttcgac ctcgacggca cgctggtcga cagcgccccc gatctcgccg
    15901 gcgccgccaa tcgcctgcgc gcggaacacg gcctgccgcc gctgccctac gaggacctgc
    15961 gccccatggt cggctcgggc gcgcgcggga tggtaggcgt ggccttcggc gtcaggccgg
    16021 gcgattcccg gttcgagcaa ttgcgcgacg cgttcctcga ccgctacgag accggcctgc
    16081 tggaacgcac gcgcccgttc gacggcgtcg aggccctgct ggcggcgctg gaagccgcgg
    16141 gcctgccctg gggcatcgtc accaacaagg cgacgcgctt cacgcgcccc atcgtcgagg
    16201 gcctgggcct ggcggcgcgc gcggccgcgg tggtctgcgg cgacacgacg ccgcacgcga
    16261 agccgcatcc cgcgccgctg ctgcatgcgg cgcaggcgat gggcgtcccg cccgagggcc
    16321 tggcctacgt cggcgacgac ctgcgcgacg cccaggccgc gcgcgcggcc ggcatgcgca
    16381 tggtcgcggc cgcctggggc tacctg
//
LOCUS       DASVLN010000016         5514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_54571, whole genome shotgun sequence.
ACCESSION   DASVLN010000016 DASVLN010000000
VERSION     DASVLN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54571"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VF453_00370"
     CDS             <1..280
                     /locus_tag="VF453_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEX7686119.1"
                     /translation="QPGDVLVAFALRPDDPQLAAMIAAAHEREMSAVVLAGRDVHGAG
                     RTTAGAAETDLWITVPHERAARVCELQLLTLHALCDAVDLQLLGDADL"
     gene            290..931
                     /locus_tag="VF453_00375"
     CDS             290..931
                     /locus_tag="VF453_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7686120.1"
                     /translation="MTLRTVTSDRRHPLVSTMLAGALVLGTLASLQGCVLLLGAGAVG
                     SAVVLTDRRTSAAQLEDESIELKGGGRVREVLGDQGHVNVTSYNRLVLLSGEVPTEAD
                     KAAVEKAVAGIDNVTSVVNELEVGENSSLKTRSSDTLITTRVKSALVDAKDLQASAIK
                     VVTERGNVYLMGRVTEREAARATEVARSQSSVMKVVRVFEILTEDQLANLRNG"
     gene            complement(969..1865)
                     /locus_tag="VF453_00380"
     CDS             complement(969..1865)
                     /locus_tag="VF453_00380"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7686121.1"
                     /translation="MAKTYESVAPRKVAFLGLGVMGGPMAGHLKAAGHEVTVYNRTAA
                     KAQAWVAQHGGRAAATPREAAEGAEFVFCCVGNDADLRAVVTGDDGAFAGMARGTVLV
                     DHTTASADVARELHGKARLRGLQFVDAPVSGGEAGAVNGLLTVMCGGEAAAFEAMKPV
                     AMAFARAITRLGESGSGQLAKMVNQICIAGIVQGLSEAIAFGTRAGLDMPAVLDVIGK
                     GAAQSWQMDNRGKTMVEDKFDFGFAVDWMRKDLGLVLDEARRNGARLPLTALVDQFYA
                     ELQVRGGGRWDTSSLIRLVSGK"
     gene            complement(1868..3004)
                     /locus_tag="VF453_00385"
     CDS             complement(1868..3004)
                     /locus_tag="VF453_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7686122.1"
                     /translation="MEREQASKFVNDLLRLMVTRNGSDLFLTADFPPAIKVDGKVTKV
                     SPQPLTGAHTMALARAIMNDKQASDFERTKECNFAISPQGIGRFRCSAFLQQGHVGIV
                     LRTIPQEIPTIDSLGLPRVLKDVALTKRGLLIMVGATGSGKSTSQAAMLDWRNEHTHG
                     HIITVEDPVEFVHPHKNCIVTQREIGIDTDTWENALKNALRQAPDVILMGEIRDRETM
                     DLAIAFAETGHLCMATLHANSANQALDRMLNFFPEERRQQLLMDLSLNLKALISQRLL
                     PRQEGKGRIAAVEILLNSPLIADLIFQGKVGEIKDLMKRSRELGMQTFDQALFDLYEA
                     NMVTYEDALRNADSVNDLRLQIKLDSERSRATDLAAGTEHLSII"
     gene            complement(3124..3765)
                     /locus_tag="VF453_00390"
     CDS             complement(3124..3765)
                     /locus_tag="VF453_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7686123.1"
                     /translation="MKSFLARFRRKPAPLAVTPRDDSSSYFATQFQEQHQDTQLMVSW
                     DARSLDLHTKPYDPALGTLALVKLFGQDPQLSQLGPEALAVFGNYLDYVQVEPGRQVI
                     GQDEQGDFLLIVLDGTIAVERVQPWGAKARLGESRAGDVLGEMSLLDAGARFSACTTL
                     TPAKFAVLGAAALDRMMQHEPRLAAALLAWLARRLSLRLRQVSARLSALLTRE"
     gene            complement(3796..4839)
                     /locus_tag="VF453_00395"
     CDS             complement(3796..4839)
                     /locus_tag="VF453_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX7686124.1"
                     /translation="MDITQLLAFSVKNKASDLHLSSGLPPMIRVHGDVRRINIDPMSH
                     KQVHGLVYDIMNDTQRKHFEDTLECDFSFEIQGLARFRVNAFNQNRGAGAVFRTIPSK
                     ILTLEQLNCPRVFSELAMKPRGLVLVTGPTGSGKSTTLAAMIDHLNDNSYGHVLTIED
                     PIEFVHESKKCLINQREVGPQTLSFSNALRSALREDPDAILVGELRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTIDRVVDVFPAAEKEMVRAMLSESLIGVISQTLCKTKDGAG
                     RVAAHEIMLGTAAIRNLIRENKIAQMYSSIQTGNQAGMQTLDQCLADLVRRNIIAPAE
                     ARQKAKFPENFPG"
     gene            4893..>5514
                     /locus_tag="VF453_00400"
     CDS             4893..>5514
                     /locus_tag="VF453_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX7686125.1"
                     /translation="MASIAENILQVRARISSACRAFHRVGDTVQLLAVSKTCPADAVR
                     EAHAAGQHDFGENYVQEAVAKIEALADLRAEMRWHLIGPLQSNKTREVATNFDWVHSV
                     DRLKLAQRLSDQRPAALPPLQVCLQVNISGEASKSGAAPSEVPELARAVQALPRLKLR
                     GLMAIPEPAGDLEAQRAPHRALRRLLDALNAQGLGLDTLSIGMSADL"
BASE COUNT          796 a         1899 c         1953 g          866 t
ORIGIN      
        1 acaaccgggc gacgtgctcg tggccttcgc gctgcggccg gacgaccccc agctcgcggc
       61 gatgatcgcc gccgcgcacg agcgcgagat gagcgccgtg gtgctcgccg gccgcgacgt
      121 ccacggcgcc ggccgcacga ccgccggcgc ggccgagacc gacctgtgga tcaccgtccc
      181 gcatgaacgt gccgcgcgcg tgtgcgagct ccagctgctg acgctgcacg ccctgtgcga
      241 tgccgtcgac ctccaactcc tcggcgatgc cgacctctga acgaacgcca tgaccctgcg
      301 caccgtcact tccgatcgcc gccatccgct ggtctccacc atgctcgccg gcgcgctggt
      361 tctgggcacg ctcgcctcgc tgcagggctg cgtgctgctg ctcggggccg gcgccgtcgg
      421 cagcgccgtc gtcctgaccg accgccgcac ctccgcggcg cagctggagg acgagtcgat
      481 cgagctgaag ggcggcggcc gcgtgcgcga ggtgctgggc gaccagggcc atgtcaacgt
      541 gacgagctac aaccgcctcg tgctgctttc cggcgaagtg ccgaccgagg ccgacaaggc
      601 cgccgtcgag aaggccgtcg ccggcatcga caacgtgacc tcggtcgtca acgagctcga
      661 ggtcggcgag aacagctcgc tgaagacgcg ctccagcgac acgctcatca cgacgcgcgt
      721 gaagagcgcg ctggtcgacg cgaaggactt gcaggcgagc gcgatcaagg tcgtcaccga
      781 gcgcggcaac gtctacctga tgggccgcgt gacggagcgc gaggccgcac gcgcgaccga
      841 agtggcacgc agccagtcca gcgtgatgaa ggtggtgcgg gtattcgaga tcctcaccga
      901 ggaccagctc gccaacctgc gcaacggctg acggcgggcg cgcaaggtcg ccgcgcgtcg
      961 ccttcgcatc acttgccgct gacgaggcgg atcaggctgg acgtgtccca gcgcccgccg
     1021 ccgcgcacct gcagctccgc gtagaactgg tcgacgagcg ccgtcagcgg cagccgggct
     1081 ccgttgcggc gcgcctcgtc gagcacgagg cccaggtcct tgcgcatcca gtcgacggcg
     1141 aagccgaagt cgaacttgtc ctcgaccatc gtcttgccgc ggttgtccat ctgccagctc
     1201 tgcgccgcgc ccttgccgat gacgtcgagg acggccggca tgtccaggcc cgcgcgggtg
     1261 ccgaaggcga tcgcctcgga cagcccctgc acgatgccgg cgatgcagat ctggttgacc
     1321 atcttggcga gctggcccga gccgctctcg cccaggcgcg tgatcgcgcg cgcgaaggcc
     1381 atcgcgaccg gcttcatcgc ctcgaaggcc gcggcctcgc cgccgcacat gaccgtcagc
     1441 aggccgttga ccgcgcccgc ctcgccgccc gagaccggcg cgtcgacgaa ctgcagcccc
     1501 cgcaggcgtg ccttgccgtg cagctcgcgc gcgacgtcgg cggacgccgt cgtgtggtcg
     1561 accagcaccg tgccgcgcgc catgccggcg aacgcgccgt cgtcgccggt gacgaccgcg
     1621 cgcaggtcgg cgtcgttgcc gacgcagcag aagacgaact cggcgccctc ggccgcctcg
     1681 cgcggcgtcg cggccgcccg gccgccgtgc tgcgcgaccc aggcctgggc cttcgcggcc
     1741 gtgcggttgt agaccgtcac ctcgtgcccg gcggccttca ggtgcccggc catcggaccg
     1801 cccatcacgc ccagcccgag gaacgcgacc ttgcgcggcg cgacggattc gtaggtcttg
     1861 gccatgttca gatgatcgac agatgttcgg tgccggcggc gaggtcggtc gcgcgggagc
     1921 gctcgctgtc cagcttgatc tgcagccgca ggtcgttgac cgagtcggcg ttgcgcaggg
     1981 cgtcctcgta ggtgaccatg ttggcctcgt agaggtcgaa cagcgcctgg tcgaaggtct
     2041 gcatgccgag ctcgcgcgag cgcttcatca ggtccttgat ctcgcccacc ttgccctgga
     2101 agatcaggtc ggcgatcagc ggcgagttca gcaggatctc gaccgccgcg atgcggccct
     2161 tgccctcctg gcgcggcagc aggcgctgcg agatcagcgc cttcaggttc agcgacaggt
     2221 ccatcagcag ctgctggcgg cgctcctcgg ggaagaagtt gagcatccgg tcgagcgcct
     2281 ggttggccga gttcgcgtgc agggtggcca tgcacaggtg gcctgtctcc gcgaacgcga
     2341 tcgcgaggtc catcgtctcg cggtcgcgga tctcgcccat caggatcacg tccggcgcct
     2401 gccgcagggc gttcttcagc gcgttctccc aggtgtcggt gtcgatgccg atctcgcgct
     2461 gggtcacgat gcagttcttg tgcgggtgca cgaactcgac cggatcctcc accgtgatga
     2521 tgtggccgtg cgtgtgctcg ttgcgccagt ccagcatcgc ggcctggctg gtcgacttgc
     2581 ccgagccggt cgcgccgacc atgatcagca gcccgcgctt ggtcagcgcg acgtccttga
     2641 gcacgcgggg aaggccgagg ctgtcgatgg tggggatctc ctgcgggatc gtccgcagca
     2701 cgatgcccac gtgcccctgc tgcaggaatg cgctgcagcg gaagcggccg atgccctgcg
     2761 gcgagatcgc gaagttgcac tccttcgtgc gctcgaagtc ggacgcctgc ttgtcgttca
     2821 tgatcgcgcg ggccagcgcc atcgtgtgcg cgccggtcag cggctgcggc gagaccttgg
     2881 tgaccttgcc gtcgaccttg atcgcgggcg ggaagtccgc ggtcaggaac aggtcggagc
     2941 cgttgcgcgt gaccatcagg cgcagcaggt cgttgacgaa cttggatgct tgctctcttt
     3001 ccatggcctg ccttcttccc tgttgacccg gcggcgcgcc gggcgaccct gcgcgcgcgg
     3061 ggtcggacta ccttgatcta cgacattgtg cgccgtccat cgagggcgac gcgcggacgg
     3121 atttcactcc cgcgtgagca gcgccgacaa gcgcgcgctc acctggcgca ggcgcagcga
     3181 gagccggcgc gcgagccacg cgagcagcgc cgccgccagg cgcggctcgt gctgcatcat
     3241 gcggtcgagc gccgcggcgc cgagcacggc gaacttcgcg ggggtgagcg tggtgcaggc
     3301 cgagaagcgg gcgccggcat cgagcagcga catctcgccc agcacgtcgc cggcgcgcga
     3361 ctcgcccagg cgcgccttcg cgccccaggg ctgcacgcgc tcgacggcga tggtgccgtc
     3421 cagcacgatc agcaggaagt cgccctgctc gtcctggccg atgacctgcc gccccggctc
     3481 gacctgcacg tagtcgaggt agttgccgaa caccgccaac gcctcgggcc cgagctgcga
     3541 cagctgcgga tcctgcccga acagcttgac cagcgcgagc gtgccgagcg ccgggtcgta
     3601 gggcttggtg tgcaggtcga ggctgcgcgc gtcccagctg accatgagct gcgtgtcctg
     3661 gtgctgctcc tggaactgcg tggcgaagta gctcgacgaa tcgtcgcgcg gggtgacggc
     3721 gagcggcgcc ggcttgcggc ggaagcgggc gaggaagctt ttcacggggc ggcgacgtcg
     3781 gcaggcggcg gggcttcagc ccgggaagtt ctcggggaac ttggccttct ggcgagcctc
     3841 cgccggcgcg atgatgttgc gccggacgag gtccgcgagg cactggtcga gcgtctgcat
     3901 cccggcctgg ttgccggtct ggatcgacga gtacatctgc gcgatcttgt tctcgcggat
     3961 gaggttgcgg atggccgccg tgcccagcat gatctcgtgc gccgcaacgc ggccggcgcc
     4021 gtccttcgtc ttgcacagcg tctgcgagat cacgccgatc agcgactccg acagcatcgc
     4081 ccgcaccatc tccttctcgg cggccgggaa gacgtcgacc acgcggtcga tcgtcttggc
     4141 ggcgctggag gtgtgcagcg tgccgaagac caggtgcccc gtctcggccg cggtcagggc
     4201 gaggcggatg gtctcgaggt cgcgcagctc gcccaccagg atcgcgtccg gatcctcgcg
     4261 cagcgccgag cgcagcgcgt tcgagaagct gagcgtctgc ggcccgacct cgcgctggtt
     4321 gatcaggcac ttcttcgact cgtggacgaa ctcgatcgga tcctcgatcg tcagcacgtg
     4381 gccatagctg ttgtcgttga ggtggtcgat catcgcggcc agcgtcgtcg acttgccgga
     4441 gccggtcggg ccggtcacca gcaccaggcc gcgcggcttc atcgcgagct ccgagaacac
     4501 gcgcgggcag ttcagctgct ccagcgtgag gatcttggag ggaatcgtcc ggaacacggc
     4561 gccggcgccg cggttctggt tgaacgcgtt gacgcggaaa cgtgcgaggc cctggatctc
     4621 gaacgagaag tcgcactcca gcgtgtcctc gaagtgcttg cgctgggtgt cgttcatgat
     4681 gtcgtacacc aggccgtgga cctgcttgtg cgacatgggg tcgatgttga tccgccggac
     4741 gtccccgtgc acccggatca tgggcggcag ccccgaggac aggtgcaggt cggaggcctt
     4801 gttcttgacc gagaacgcga gcagttgggt gatgtccatt ggggcggcgg gaatcaggag
     4861 gcgacgggca ggcttggctt atgctcgggc cgatggccag catcgccgag aacatactac
     4921 aggtccgggc tcgaatttcg tccgcctgcc gggcatttca ccgggttggt gacacggtgc
     4981 aactgctcgc cgtgagcaag acctgccccg ccgacgccgt gcgcgaggcg cacgccgccg
     5041 gccagcacga cttcggcgag aactacgtgc aggaggcggt cgccaagatc gaggcgctcg
     5101 cggacctgcg cgccgagatg cgctggcacc tgatcggccc gctgcagagc aacaagacgc
     5161 gcgaagtggc gaccaacttc gactgggtgc attcggtcga ccggctcaag ctggcgcagc
     5221 gcctgtccga ccagcgcccg gccgcgctgc cgccgctgca ggtctgcctg caggtcaaca
     5281 tcagcggcga ggcgagcaag agcggcgcgg cgccgtccga ggtacccgag ctcgcgcggg
     5341 ccgtgcaggc gctgccgcgg ctgaagctgc gcgggctgat ggcgatcccc gagccggcgg
     5401 gcgacctcga ggcccagcgt gcgccgcacc gggcgctgcg gcggctgctg gacgcgctca
     5461 acgcgcaagg tctcgggctg gacacgctgt ccatcggcat gagcgccgac ctcg
//
LOCUS       DASVLN010000017         6642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_215730, whole genome shotgun sequence.
ACCESSION   DASVLN010000017 DASVLN010000000
VERSION     DASVLN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215730"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /gene="dapA"
                     /locus_tag="VF453_00405"
     CDS             complement(<1..725)
                     /gene="dapA"
                     /locus_tag="VF453_00405"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX7686126.1"
                     /translation="MERIVGSLVALVTPMHEDGSVDYPALRALVDWHVAEGTDCIVAV
                     GTTGESATLSVEEHVEVIRVCVEQAAGRMPIMAGAGANSTAEAIELTRHAQAVGASCT
                     LQVVPYYNKPSQEGMYRHFRKIAESVDLPVVLYNVPGRTVADLQHDTVVRLSQVPGIV
                     GIKEATGSIERAGWLLRAVPKSFSVYSGDDSTAVALMLLGGHGNISVTANVAPRAMHE
                     LCDAALAGDARRAAELHLKLLPL"
     gene            complement(848..1498)
                     /locus_tag="VF453_00410"
     CDS             complement(848..1498)
                     /locus_tag="VF453_00410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7686127.1"
                     /translation="MTHELRHGTVAAPSEWIERWTPLLAPGSSVLDVACGSGRNARWL
                     ARAGHRVTAVDRDAEALAVVSLEASEPGVVLHTLLADLEGAPWPLPGERFDAVVVTNY
                     LWRPLLPALLEAVAPGGLLLYETFALGQAELGRPRNPDYLLRPNELITLLREPVPAFP
                     PGSAAGAVPAMPWHVVAFEEGRLPGSAAAPPREVQRIVARRGKSPRERPDLLVAPG"
     gene            complement(1495..1746)
                     /locus_tag="VF453_00415"
     CDS             complement(1495..1746)
                     /locus_tag="VF453_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686128.1"
                     /translation="MKSRIPHVSLAPPALESPSRFASRRGLDGGAPLSPRSRRARVLR
                     RLRRVAWAVGVLAALAAVFMSYFDRDLLFTLASQAWSCL"
     gene            2083..3981
                     /locus_tag="VF453_00420"
     CDS             2083..3981
                     /locus_tag="VF453_00420"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX7686129.1"
                     /translation="MYTRTDNPLHIVQEVIDNAADEALAGHGRRIAVTLHADGSVTVD
                     DDGRGIPFGLHPEEQVPVLEIVYTRLHAGGKFDKGSGGAYSFSGGLHGVGVSVTNALA
                     RQLEVHVFRDGQFASLTFAGGDVSVPLAVRKLAAGERRHGTRVRVWPDPRYFESAELP
                     RGELVHLLRSKAVLMPGVTVSLTIEKAGKEPEVLTWTYQGGLRDYLQQTLTSEPIVPL
                     FDGEHYAGANESENFAEGEGATWCVAFTDDGAAVRESYVNLIPTVAGGTHESGLKDGL
                     FTAVKSFIELHGLLPKGVKLMPEDVFSRASFVLSAKVLDPQFQGQTKERLNSRDALRL
                     VSAFTKPALELWLHQHVEYGRKLAELVIRQAQARQRAGQKVEKRKGSGVAVLPGKLTD
                     CESRDLAHNEVFLVEGDSAGGSAKMGRDKETQAILPLRGKVLNTWEVERDRLFANTEI
                     HDIAVAVGVDPHGPDDTPDFSGLRYGKVCILSDADVDGSHIQVLLLTLFFRHFPQLVA
                     RGHVYVAKPPLYRIDAPARGRKPAAKIYALDEGELEATLDKLRKEGARDGSWKISRFK
                     GLGEMNAEQLWETTLNPETRRLLRVQLGALDASQTEGSFTRLMGKGEAQARRDLMELR
                     GDAVEIDI"
     gene            4317..4892
                     /locus_tag="VF453_00425"
     CDS             4317..4892
                     /locus_tag="VF453_00425"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7686130.1"
                     /translation="MSDDVPTESAHLRIRPTMLSDLDFVVSVERDGQNLPFITPWERT
                     QHEGAIRFPDSRHFIVEAGQDWERVGFVILQGCRNPMQSIELKRIVLQTRVHGRGLGR
                     ACVRLLKGMAFRDLHAHRFWLDVKSLNTRALSLYTSEGFVEEGRLRESVRLGSDDEAR
                     WDSLVVMSLLDREYHSRVDNGLETPLPRVAR"
     gene            4889..5695
                     /locus_tag="VF453_00430"
     CDS             4889..5695
                     /locus_tag="VF453_00430"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7686131.1"
                     /translation="MRKTLLLAACTAAALVVTTAACDVLPEAGHAAPAATAAALDVTP
                     APMVVPVAGKPVLWGYVDGAGVAHFAATKLDSRYSQVLRETEGPRVLGKTTGPESLLT
                     WLEFSPDVKAMQPLLDEAARKSGVDEELLKAVITVESGYNAKAVSHAGAVGLMQLTPD
                     TADRYATKEERRRPAAERLLDARTNILTGARMLADLTRRYGGIDIALAAWNAGEGTVR
                     KAGGRMPAIAETEAHVHMVLELYWALLQRNLPQRAHRVELDGCAASATCS"
     gene            complement(5851..6570)
                     /locus_tag="VF453_00435"
     CDS             complement(5851..6570)
                     /locus_tag="VF453_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004553610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX7686132.1"
                     /translation="MSTSSLRALVFDVFGTVVDWRSGVARDARPFLERFAPGVDPFAF
                     ADAWRRRYSPAMEEVRSGRRPFARLDVLHRENLEAILPEFGIDAARVPAAALDELNLA
                     WHRLDPWPDAVPALQRLRRRFVIAPLSNGNIALMVDMARRAGLPWDAILGAEVSQAYK
                     PSPQAYLRTLEALMMRPDEVCMVAAHNGDLAAARACGLRTAFVRRPTEHGPGQETDLK
                     PQQPWDVVADDLLGVAQALGA"
BASE COUNT          988 a         2402 c         2396 g          856 t
ORIGIN      
        1 tgcagcggca gcagcttcag gtgcagctcc gcggcgcggc gtgcgtcgcc ggccagcgcc
       61 gcgtcgcaca gctcgtgcat cgcgcgcggg gcgacgttgg cggtcacgct gatgttgccg
      121 tggccgccga gcagcatcag cgcgacggcc gtgctgtcgt cgcctgagta gaccgagaac
      181 gacttcggca cggcgcgcag cagccagccc gcgcgctcga tgctgccggt ggcctccttg
      241 atgcccacga tgccgggcac ctgcgaaagg cggacgacgg tgtcgtgctg caggtcggcc
      301 accgtgcggc cgggcacgtt gtacagcacc accggcaggt cgaccgactc ggcgatcttg
      361 cggaagtgcc ggtacatgcc ctcctgcgac ggcttgttgt agtagggcac gacctgcagc
      421 gtgcagctcg cgccgaccgc ctgcgcgtgg cgcgtgagct cgatcgcctc ggccgtcgaa
      481 ttcgcgccgg cgccggccat gatgggcatg cggcccgccg cctgctcgac gcacacgcgg
      541 atcacctcga cgtgctcctc gacgctcagc gtcgccgact cgccggtggt gccgaccgcg
      601 acgatgcagt ccgtgccctc ggccacgtgc cagtcgacca gcgcgcgcag cgccgggtag
      661 tcgacgctgc cgtcctcgtg catcggggtg accagcgcca cgaggctgcc gacgattcgt
      721 tccattcgca ttccttggga gatgtccttc gcgcccggcg cgttgcgcgc cgacgcgggt
      781 cgcgggcgcg gacggccggc ggcgcgcatt ccgggccggg gtcggccgcg gaggcaatcc
      841 gatgatttta gcccggggcg accagcagat cgggccgttc ccgcggcgac tttccgcgcc
      901 gcgcgacgat tcgctgcacc tcgcgcggcg gcgccgccgc cgatccgggc agccggccct
      961 cctcgaaagc cacgacgtgc cagggcatgg cgggcacggc gccggccgcg ctgccgggcg
     1021 gaaacgccgg cacgggctcc cgcagcagcg tgatcagctc gttcgggcgc agcaggtagt
     1081 cggggttgcg cggtcgcccg agttccgcct ggccgagtgc aaatgtttcg taaagaagca
     1141 ggccgccggg ggcgaccgcc tccagcagcg cgggcaacag cggtcgccac aggtagttcg
     1201 tgacgacgac ggcgtcgaag cgctcgccgg gcaggggcca gggcgcgcct tcgaggtcgg
     1261 ccagcagcgt gtgcagcacg acgcccggct ccgacgcctc cagcgagacg accgcgagcg
     1321 cctcggcgtc gcggtcgacg gcagtgactc gatgccccgc gcgcgcgagc cagcgcgcgt
     1381 tgcggcccga gccgcaggcg acgtccagca cggacgagcc cggggcgagc agcggcgtcc
     1441 agcgctcgat ccattccgag ggcgcggcca ccgtgccatg tcgcagctcg tgggtcacag
     1501 gcagctccag gcctggctcg cgagcgtgaa cagcaggtcg cgatcgaagt aggacatgaa
     1561 caccgctgcg agcgccgcca ggacgccgac cgcccaggcg actcgccgca gccggcgcag
     1621 cacgcgcgca cggcgcgagc gcggggacag cggcgcgccg ccgtccaggc cgcggcgaga
     1681 ggcgaagcgg ctggggctct cgagcgccgg tggcgcgagg ctgacgtggg ggatgcgcga
     1741 cttcatgatg gcgtcaggac gaagcggccg gcgggccgac gccggccgga gcgcggacga
     1801 tggccgcccc gcacaggagc acctgggcca gggacagcgg ggggcgggaa tcggcatgca
     1861 tgggggacga ctctacaggc tcgccgggac gccctgcacg caatttcctg tgtttatgta
     1921 cagatttgac gtccaggccg gcgcgcgccg gatcctagaa tcgacgccat ggccacgaag
     1981 cagggcgccc cgggcgccaa atccaccaag caagccggcg agtacggcga agcctccatc
     2041 cgcgtgctga agggcctcga gcccgtcaag cagcggccgg gcatgtacac gcgcaccgac
     2101 aacccgctgc acatcgtcca ggaggtgatc gacaacgccg ccgacgaggc gctcgccgga
     2161 cacggccgcc gcatcgccgt cacgctgcac gcggacggct cggtgacggt cgacgacgac
     2221 ggccgcggca tcccgttcgg gctgcatccc gaggagcagg tgccggtcct cgagatcgtc
     2281 tacacgcgcc tgcacgcggg cggcaagttc gacaagggca gcggcggcgc ctacagcttc
     2341 tccggcggcc tgcacggcgt gggcgtgagc gtgaccaacg cgctcgctcg ccagctcgag
     2401 gtccatgtct tccgggacgg ccagttcgcc tcgctgacgt tcgcgggcgg cgacgtgtcg
     2461 gtgcccctgg cggtgcgcaa gctcgcggcc ggcgagcgcc gccacggcac gcgcgtgcgc
     2521 gtctggcccg atccgcgcta tttcgagagc gccgagctgc cgcgcggcga gctcgtgcac
     2581 ctgctgcgca gcaaggcggt gctgatgccg ggcgtgacgg tgtcgctgac gatcgagaag
     2641 gccggcaagg agcccgaggt gctgacctgg acgtaccagg gcggcctgcg cgactacctc
     2701 cagcagacgc tcacctcgga gccgatcgtc ccgctgttcg acggcgagca ctatgccggg
     2761 gccaacgaat cggagaactt cgcggaaggc gagggagcga cctggtgcgt cgccttcacc
     2821 gacgacggcg cggcagtgcg cgagagctac gtcaacctga tcccgacggt ggccggcggc
     2881 acgcacgaat ccggcctcaa ggacggcctg ttcaccgcgg tcaagagctt catcgagctg
     2941 cacggcctgc tgcccaaggg cgtcaagctg atgcccgagg acgtcttctc gcgcgcaagc
     3001 ttcgtgctct cggccaaggt gctcgacccc cagttccagg gccagacgaa ggagcgcctg
     3061 aacagccgcg acgcgctgcg cctggtctcc gccttcacca agccggcgct ggagctgtgg
     3121 ctgcaccagc acgtcgagta cggccgcaag ctcgccgagc tcgtgatccg ccaggcgcag
     3181 gcgcgccagc gcgccggaca gaaggtcgag aagcgcaagg gctcgggcgt ggcggtgctg
     3241 cccggcaagc tgaccgactg cgagagccgc gacctggcgc acaacgaggt gttcctggtc
     3301 gagggcgact ccgcgggcgg ctcggccaag atgggccgcg acaaggagac ccaggcgatc
     3361 ctgcccctgc gcggcaaggt cctcaacacc tgggaggtcg agcgcgaccg cctgttcgcc
     3421 aacaccgaga tccacgacat cgcggtggcc gtgggcgtgg atccgcacgg gcccgacgac
     3481 acgcccgact tctcgggcct gcgctatggc aaggtctgca tcctgtcgga cgcggacgtc
     3541 gacggctcgc acatccaggt gctgctgctg accctgttct tccgccactt tccgcagctg
     3601 gtggcgcgtg ggcacgtgta tgtcgccaag ccgccgctgt accgcatcga tgcgccggcc
     3661 cgcggccgca agccggcggc caagatctat gcgctcgacg aaggcgagct cgaagccacg
     3721 ctggacaagc tgcgcaagga gggcgcgcgt gacggctcct ggaagatcag ccgcttcaag
     3781 ggcctgggcg agatgaacgc cgagcagctc tgggaaacga cgctcaaccc cgagacccgc
     3841 cgcctgctgc gggtgcagct gggcgcgctg gacgcgagcc agaccgaagg ctcgttcacg
     3901 cggctgatgg gcaagggcga ggcgcaggcg cggcgcgacc tgatggagct gcgcggggac
     3961 gcggtcgaga tcgacatctg agcatcggcc gaccggccga tccgtcgcgg gcgccacgcc
     4021 ggcccgcgcg tccgagcggt gcgcaacggc gctccgcggc cgcccgcagg cgcagctcgc
     4081 gccggacgtg ggcgcttcct aaccgcgcag tccagcgccg aacggacgag ccaagtgcgc
     4141 gcccctcccg cttttcgtgc ggaaatgccg cttttctcgg gggatcgccc gccgcagggc
     4201 ctgcgatcct tgcctctaga atccaccgct ccagccccgg gcgacaccgt cgcgcagggc
     4261 acccgcctgc cgtgctgccc ttcctcgccg ccttcagcct gcccgcccgc cgccccatgt
     4321 ccgacgacgt cccgaccgag agcgcgcatc tgcggatccg tcccacgatg ctgtccgacc
     4381 tcgacttcgt cgtgtcggtc gagcgcgacg gccagaacct gccgttcatc acgccgtggg
     4441 agcgcaccca gcacgagggc gcgatccgct tccccgactc gcgccacttc atcgtcgagg
     4501 cggggcagga ctgggagcgc gtcggcttcg tgatcctgca gggctgccgc aacccgatgc
     4561 agtcgatcga gctcaagcgc atcgtgctgc agacccgcgt ccacggccgc ggcctcgggc
     4621 gtgcctgcgt gcggctgctc aagggcatgg ccttccgcga cctgcacgcg caccgcttct
     4681 ggctcgacgt gaagtcgctc aacacgcggg cactgagcct gtacacgagc gaaggcttcg
     4741 tcgaggaagg ccggctgcgc gagagcgtgc gactgggctc cgacgacgag gcgcgctggg
     4801 acagcctggt cgtcatgtcg ctgctcgatc gcgagtacca ctcccgggtc gacaacggcc
     4861 tcgagacgcc gctgccgagg gtcgcgcgat gaggaagacg ctcctgctgg cggcctgcac
     4921 ggccgccgcg ctcgtcgtca cgaccgcggc ctgcgacgtg ctgcccgaag ccggccacgc
     4981 cgccccggcc gcgacggccg ccgccctcga cgtaacgccg gcgccgatgg tcgtgccggt
     5041 cgccggcaag ccggtgttgt ggggctatgt cgacggcgcg ggcgtcgccc atttcgcggc
     5101 gacgaagctg gactcccgct actcccaggt tctgcgcgag accgagggcc cgcgcgtgct
     5161 cggcaagacg accggccccg agagcctgct gacctggctc gagttctccc ccgacgtgaa
     5221 ggcgatgcag cccctgctgg acgaggcggc gcgcaagagc ggcgtcgacg aggagctgct
     5281 gaaggccgtc atcacggtcg agtcgggcta caacgccaag gccgtctccc acgcgggcgc
     5341 ggtcgggctg atgcagctca cgcccgacac cgccgaccgc tacgcgacca aggaggaacg
     5401 tcgccgcccc gccgccgagc gcctgctcga cgcgcgcacc aacatcctga ccggcgcccg
     5461 catgctggcc gacctcacgc gccgctacgg tggcatcgac atcgcgctgg ccgcctggaa
     5521 cgccggcgaa ggcaccgtgc gcaaggcggg gggccgcatg ccggcgatcg ccgagaccga
     5581 ggcccatgtc cacatggtgc tggagctgta ctgggccctg ctgcagcgca acctgccgca
     5641 acgggcgcac cgcgtcgagc tggacggctg cgccgcgagc gcgacctgca gctgacgacg
     5701 ctcggccggc ggcgtccggt ggtcggatga cgggcgcgca gcgcagcgca cctggtcaag
     5761 gccaggatgc gcgaagcgca ggcccgcggc gcccctgcga tcagcgcgac cacggtgctt
     5821 cgagtggctt gcgggcgcct tccggatgac tcaggcgccg agcgcctgcg ccacgcccag
     5881 gaggtcgtcg gcgacgacgt cccagggctg ctgcggcttc aggtcggtct cctggccggg
     5941 cccatgctcg gtcggccgcc ggacgaacgc cgttcgcagg ccgcacgccc gcgcggcggc
     6001 gaggtcgccg ttgtgcgccg ccaccatgca gacctcgtcg gggcgcatca tgagcgcctc
     6061 cagcgtgcgc aggtaggcct gcggcgaagg cttgtaggcc tgcgagacct ccgcgccgag
     6121 gatcgcatcc caaggcaggc cggcgcgcct ggccatgtcg accatcagcg cgatgttgcc
     6181 gttcgacagc ggggcgatga cgaagcgccg cctgagccgc tgcagcgccg gcaccgcgtc
     6241 cggccacggg tccaggcgat gccaggcgag gttgagctcg tccagcgccg cggccggcac
     6301 ccgggcggcg tcgatgccga actccggcag gatggcctcg aggttctccc ggtgcaggac
     6361 gtcgagccgc gcgaacggcc ggcggccact gcgcacttcc tccatcgccg gcgaatagcg
     6421 gcgccgccag gcgtccgcga aggcgaacgg atcgaccccc ggcgcgaaac gctcgaggaa
     6481 agggcgcgcg tcgcgcgcca cgccgctgcg ccagtccacg accgtgccga agacatcgaa
     6541 gaccagggcg cgcagggagc tcgtggacat ggcgcctttc acgggatggg cgcgctcagg
     6601 ccgcgccgcg tgcgtcatcg gccgctcgcg cgacgagcgc cc
//
LOCUS       DASVLN010000018         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_108579, whole genome shotgun sequence.
ACCESSION   DASVLN010000018 DASVLN010000000
VERSION     DASVLN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108579"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /gene="rsmH"
                     /locus_tag="VF453_00440"
     CDS             complement(<1..857)
                     /gene="rsmH"
                     /locus_tag="VF453_00440"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7686133.1"
                     /translation="MDTPGLHTTVLLHEAIDALVTRPEGTYVDGTFGRGGHTRALLAR
                     LGPGARVIAVDRDPEAIAAATTGPDAIADPRFEVVHASFAQLPQALAERGVERIDGLL
                     LDLGVSSPQIDTPERGFSFRFDGPLDMRMDTTRGETAADFLARADERHIAEVIRDYGE
                     ERFAVSIAKAIVAGRQAGAPVRTTRELSALVARAVKTREPGQDPATRTFQALRILVNA
                     ELEELEQVLKSALDLLAPGGRLAVISFHSLEDRIVKTFIARESKAEIDRRAPFAPPQV
                     ALRLRALGR"
     gene            complement(863..1291)
                     /gene="mraZ"
                     /locus_tag="VF453_00445"
     CDS             complement(863..1291)
                     /gene="mraZ"
                     /locus_tag="VF453_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7686134.1"
                     /translation="MFEGPSSLTLDGKGRVSMPSRYRDDLQALCAGRLTVTKHPHGCL
                     MIFPRPAWEAFREQLAQLPFSQVGVKRVFLGHADDVEMDATGRILIHPDLRTFATLER
                     DVKLMGMGSHFELWAAPAYAAYEARVMQAEASAALDELRF"
     gene            complement(1641..>1857)
                     /locus_tag="VF453_00450"
     CDS             complement(1641..>1857)
                     /locus_tag="VF453_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686135.1"
                     /translation="PPPPVASAPKPAPAPARSLTEVCNDKASHASIFAKSWEMTSCMR
                     QGCCGTSRQNSQECVDFDKRFPLNCPR"
BASE COUNT          308 a          597 c          667 g          285 t
ORIGIN      
        1 atgcgcccga gcgcgcgcag gcgcagcgcg acctggggcg gcgcgaacgg cgcgcggcgg
       61 tcgatctcgg ccttgctctc gcgcgcgatg aaggtcttga cgatgcgatc ctcgagcgaa
      121 tggaagctga tgaccgccag gcggccgccc ggcgcaagca ggtccagcgc ggacttcagg
      181 acctgctcga gctcctccag ttcggcattg acgagaatcc gaagagcttg aaaggtgcgc
      241 gtggcgggat cctggcccgg ctcgcgggtc ttgaccgcac gagccacgag ggcggacagc
      301 tcgcgagtgg ttcgcacggg tgcgcctgcc tgccggccag caacaatcgc ctttgcaatc
      361 gatacagcaa accgttcttc tccatagtcg cgtatcacct cggcaatgtg gcgctcgtcg
      421 gcgcgagcga ggaagtccgc ggccgtctcg ccccgcgtcg tgtccatgcg catgtccagc
      481 gggccgtcga accggaagct gaagccgcgc tcgggggtgt cgatctgcgg cgaggacacg
      541 cccaggtcga gcagcagccc gtcgatgcgc tcgacgccgc gctcggccag cgcctgcggc
      601 agctgggcga agctcgcgtg gacgacctcg aatcgcgggt cggcgatggc gtccgggccg
      661 gtggtggcgg cggcgatcgc ctcggggtcg cgatcgacgg cgatcacccg cgcgccgggg
      721 cccaggcgcg ccagcagcgc acgggtgtgc ccgccgcggc cgaaggtgcc gtcgacgtag
      781 gttccttccg gcctggtcac gagcgcgtcg atcgcttcgt ggagcaggac ggtggtgtgc
      841 agtcccgggg tgtccacggc ggtcagaacc tgagttcgtc gagggcggcg gacgcctcgg
      901 cctgcatcac gcgcgcctcg taggcggcgt aggccggcgc ggcccagagc tcgaagtggc
      961 tgcccatgcc catgagcttg acgtcgcgct cgagcgtcgc gaacgtgcgc aggtccgggt
     1021 ggatgaggat gcggccggtc gcgtccatct cgacatcgtc ggcatggccc aggaagacgc
     1081 gcttgacgcc cacctgggag aacggcagct gggcgagctg ctcgcggaac gcctcccacg
     1141 cggggcgcgg gaagatcatc aggcagccat gcggatgctt cgtgacggtg aggcggccgg
     1201 cgcacaatgc ctggaggtcg tcgcgataac gcgacggcat ggagactcgc cccttgccgt
     1261 ccagcgtcag cgacgaagga ccttcaaaca ccgtagattc cacgacccac cacaaaaatg
     1321 cacttttctg cactttagcg gatcgacctg gggtgggtca acgcgtcgcg ttccgaaaaa
     1381 atcaatcaaa tcaatcactt agcccacgtt ttgcaagcga atatcgttct aaatgaacaa
     1441 cttgcgcgtg gctatgaaag tgaagtctga gaagccctcg cgggccctcg aacgtggggg
     1501 atttcccgca gaaggtgctg ccgagcagcg aaatcgacgc gttggccgaa aatccgggga
     1561 ccaagaggcc cttgccgccg gctgccgcca tgaaaaaggg ccgcctcgcg ggcggccctc
     1621 gtcggcggcg ctcgaccgga tcagcgcggg cagttgagcg ggaagcgctt gtcgaagtcg
     1681 acgcactcct ggctgttctg gcgcgaggtg ccgcagcagc cctggcgcat gcaggaggtc
     1741 atctcccacg acttggcgaa gatcgaggcg tgcgaggcct tgtcgttgca gacctcggtg
     1801 aggctgcgcg ccggcgcggg cgccggcttg ggcgcgctcg cgaccggcgg gggcggc
//
LOCUS       DASVLN010000019         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_430932, whole genome shotgun sequence.
ACCESSION   DASVLN010000019 DASVLN010000000
VERSION     DASVLN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430932"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VF453_00455"
     CDS             complement(<1..359)
                     /locus_tag="VF453_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686136.1"
                     /translation="MSGIPGLGSALGDALSQAGPAAGQALQGLGAAAGSAQEKASDLA
                     SQAADAAQLAQGEATQAAQAAQQQAQQAAQAAQQQAQQAMQQAADAKSAADQAVADAG
                     NKASQAVQDAQRRAADA"
     gene            complement(408..773)
                     /locus_tag="VF453_00460"
     CDS             complement(408..773)
                     /locus_tag="VF453_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686137.1"
                     /translation="MTQAQWDRMGQDSFDARMIAIIRRNHPELASAMPLPELVAAIHR
                     QLAHARTYGLNDERSAATYVYAAWLLGEEFDRRIPALAQILRERRMTCPDKSAAIANF
                     CLLVFRALERAAPAHKKAA"
     gene            complement(952..1377)
                     /locus_tag="VF453_00465"
     CDS             complement(952..1377)
                     /locus_tag="VF453_00465"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX7686138.1"
                     /translation="MATIQVDVVSAEEQIFSGEASFVALPGESGELGIMPRHTPLITR
                     IKPGAVRIKRAGDGAEEFVFVAGGILEVQPDRVTVLADTAIRGKDLDEAKATEAKKAA
                     EAALANAKGDFDVAKAQAELAELAAQLAAIRRFRGQSAH"
     gene            complement(1452..>2199)
                     /gene="atpD"
                     /locus_tag="VF453_00470"
     CDS             complement(1452..>2199)
                     /gene="atpD"
                     /locus_tag="VF453_00470"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7686139.1"
                     /translation="PGNRLRVALTGLTIAESFRDEGRDVLFFVDNIYRYTLAGTEVSA
                     LLGRMPSAVGYQPTLAEEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFAH
                     LDATVVLSRDIASLGIYPAVDPLDSTSRQIDPNVVGEEHYGTTRAVQATLQRYKELRD
                     IIAILGMDELAPEDKLAVARARKIQRFLSQPFHVAEVFTGSPGKYVPLKETIRGFKMI
                     VAGECDHLPEQAFYMVGTIDEAFEKAKKIQ"
BASE COUNT          285 a          739 c          806 g          369 t
ORIGIN      
        1 tcggcatcgg cggcgcgccg ctgcgcgtcc tgcaccgcct gcgaggcctt gttcccggca
       61 tcggcgaccg cctggtccgc ggcgctcttc gcgtcggcgg cctgctgcat cgcctgctgc
      121 gcctgctgct gcgcggcctg cgccgcctgt tgggcctgct gctgcgcggc ctgggcggcc
      181 tgggtcgcct cgccttgcgc caactgggcg gcgtcggcgg cctggctggc gaggtcggac
      241 gccttctcct gcgccgagcc cgccgccgcg ccgaggcctt gcagcgcctg gcccgccgcc
      301 gggccggctt gcgacaacgc gtcgcccaac gcactgccga gtccagggat gccgctcatc
      361 ggatgacctc cgtccgtcga atgggaaatc gggaacgcga agcgccttca ggccgccttc
      421 ttgtgtgccg gcgccgcgcg ctccagggcg cggaagacca gcaggcagaa attcgcgatc
      481 gcggccgact tgtccggaca ggtcatccgc cgctcgcgca ggatctgggc cagcgcgggg
      541 atgcggcggt cgaactcctc gcccagcagc caggcggcgt agacgtaggt ggctgcggag
      601 cgttcgtcgt tgaggccgta ggtgcgcgca tgcgcgagct ggcggtggat ggccgcgacc
      661 agctcgggca acggcatcgc ggacgcgagc tcgggatggt ttcgccggat gatcgcgatc
      721 atgcgggcat cgaaggagtc ctgccccatg cgatcccatt gcgcctgagt catgcttgcc
      781 actcggccct ccgaaccgcg ggttcgtccc gctggagccc ggattgtcga tctcgccctt
      841 gcagcgacgc agtccctaga tcgcggtatc cgcccgtcgc gcaggcgtga aaaagcccgc
      901 gcacgtcgcc gtgggcgggc cttggcgtcg tcgcgtggac gacgacgcgg atcagtgcgc
      961 gctctggccg cggaagcgac ggatcgcggc gagttgcgcc gccagttcgg ccagctcggc
     1021 ctgggccttg gcgacatcga agtcgccctt ggcgttggcg agcgccgcct cggcggcctt
     1081 cttggcctcg gtggccttgg cctcgtccag gtccttgccg cggatggcgg tgtcggccag
     1141 gacggtgacg cggtcgggct gcacctcgag gatgccgcca gccacgaaca cgaattcctc
     1201 cgcgccatcg ccggcgcgct tgatgcgcac cgcgcccggc ttgatgcggg tgatcagcgg
     1261 ggtgtggcgc ggcatgatgc cgagctcgcc gctctcgccc ggcaacgcga cgaagctcgc
     1321 ctcgcccgag aagatctgct cttccgcgga gacgacgtcg acttgaatgg tggccatgtg
     1381 gatgtccgtt tcgaatggga gaagtcaggc aggacggcag atgccggcac gcgggccggc
     1441 gtccgcgcgc ctcactggat cttcttggcc ttctcgaacg cttcgtcgat ggtgccgacc
     1501 atgtagaacg cctgctccgg caggtggtcg cattcgccgg ccacgatcat cttgaagccg
     1561 cggatcgttt ccttcagcgg cacgtacttg cccggcgagc ccgtgaagac ttccgcgacg
     1621 tggaagggct gggacaggaa gcgctggatc ttgcgggcgc gggcgacggc cagcttgtct
     1681 tccggcgcga gttcgtccat gcccaggatc gcgatgatgt cgcgcagttc cttgtagcgc
     1741 tgcagcgtgg cctgcaccgc gcgggtcgtg ccgtagtgct cctcgccgac gacgttcggg
     1801 tcgatctggc gcgaggtcga gtcgagcggg tcgaccgcgg ggtagatacc cagcgaggcg
     1861 atgtcgcgcg agaggacgac ggtggcgtcc aggtgggcga aggtcgtggc gggcgacggg
     1921 tcggtcaggt cgtccgcggg gacgtacacg gcctggatcg aggtgatcga gccgaccttg
     1981 gtcgacgtga tgcgctcctg caggcggccc atttcctcgg ccagcgtcgg ctggtagccc
     2041 acggcggagg gcatgcggcc cagcagcgcg gacacttccg tgccggccag cgtgtagcgg
     2101 tagatgttgt ccacgaagaa cagcacgtcg cggccttcgt cgcggaacga ctcggcgatc
     2161 gtcaggccgg tcagcgcgac gcgcaggcgg ttgcccggc
//
LOCUS       DASVLN010000020         3988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_1137, whole genome shotgun sequence.
ACCESSION   DASVLN010000020 DASVLN010000000
VERSION     DASVLN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VF453_00475"
     CDS             complement(<1..358)
                     /locus_tag="VF453_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEX7686140.1"
                     /translation="MRHAADAPDAGLERIEAFFHGDAYSLHRHDTYAIGTTLAGVQSF
                     RYRGGLRRSLAGQTMVLHPDEPHDGHAGTDEGFRYRMLYVPPALLQEALGGVALPFVA
                     GGVSDDPRLAAATRALL"
     gene            complement(425..1282)
                     /locus_tag="VF453_00480"
     CDS             complement(425..1282)
                     /locus_tag="VF453_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX7686141.1"
                     /translation="MTMLQPAADPLADLEAYFHRIDYAGPTAPTLETLARLVDRHAHS
                     IPFENVDVLARRVPELSTAALEDKLVRRRRGGYCFEQNHLMLAVLRRLGFDARGQEAR
                     VRVGVPAERESARTHMTLRVTLAGEDWHVDVGFGSTAPAAPLRLADRGEQAAGTGRFR
                     FVDVETGLMMQSLSHEGWGDACLILPSRPAAIDYKAANWFVATHPESFLGANLLVGRA
                     VDGGRWTLFNRELSLRQPMTAAPQLRTLATRAEFEDALARHFDLRLEPPDLDAVLAAI
                     ERLPEKPAS"
     gene            complement(1279..2220)
                     /gene="ylqF"
                     /locus_tag="VF453_00485"
     CDS             complement(1279..2220)
                     /gene="ylqF"
                     /locus_tag="VF453_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEX7686142.1"
                     /translation="MPIQWFPGHMHATRKAIAERLPEIDVVIELLDARLPGSSGNPLL
                     ADMTRGKPALKILNKQDLADADATAQWLAHFGALPGTRAIGLDASVPAPARRLVEASR
                     ELVPQRGGMVKPIRVLVCGVPNVGKSTLINTITGQRVAKTGDEAGITKVEQRITLASD
                     VYLFDTPGMLWPRIGVEKAGYHLAASGAVGRNAYDEEEVALELLASVRGRYDDRLRAR
                     YKLDDIAPLADGELLEAIGRKRGALMAGGRINRTKAAEVALNDFRAGAWGRVTLESPA
                     QFLAWEAEAAAAEAEREAEREVEREQRVRARKSRDPR"
     gene            2437..2613
                     /locus_tag="VF453_00490"
     CDS             2437..2613
                     /locus_tag="VF453_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEX7686143.1"
                     /translation="MLKWALVFALVSLVAGLLGFTGLAAGAAAIAKTLFVVFAVLFLL
                     FLVLGATIFRKITD"
     gene            complement(2660..3427)
                     /locus_tag="VF453_00495"
     CDS             complement(2660..3427)
                     /locus_tag="VF453_00495"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEX7686144.1"
                     /translation="MQIRPSARSVLAALAAAAPLLAHAQVTQKPDGQWRSLFTLGATV
                     ARGNTHTDTASLQADVIRLTDHDKWAFDANGQYASSDGARTANRQSGTALYSSDFTPR
                     WFGFGQLDLLHDPLADIRSRTTLGTGVGFHAIREDKLTWDLSAGLGYTMDRYAPPVVV
                     DDELRSRYNHLDLLVAEESNHKFTDTTTFHQKLTVYPSLRNSDDVRTLFDAGLSVAMT
                     KRLALTATFEHRYDSHPAEGLGRNDTQLVTGVSLRFD"
     gene            3614..3736
                     /locus_tag="VF453_00500"
     CDS             3614..3736
                     /locus_tag="VF453_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686145.1"
                     /translation="MPAAFLEQGVQVRAIGGDHVIGYPLLRAHVRRTSRRRCSA"
BASE COUNT          492 a         1389 c         1517 g          590 t
ORIGIN      
        1 cgagcagcgc gcgcgtcgcc gcggcgagcc gggggtcgtc cgacacgccg cccgcgacga
       61 agggcaaggc gacgccgccg agggcctcct gcaacagcgc cggcggcacg tagagcatgc
      121 gatagcggaa gccctcgtcg gtgccggcgt ggccgtcgtg cggctcgtcg ggatgcagca
      181 ccatcgtctg gcccgcgagg ctgcggcgca gcccgccgcg gtagcggaac gactgcacgc
      241 ccgcgagcgt cgtgccgatc gcgtaggtgt cgtggcggtg cagcgagtag gcgtcgccgt
      301 ggaagaaggc ctcgatgcgc tcgaggccgg cgtccggcgc gtcggcggca tggcgcaccc
      361 agtcgccgcg gtggcggcgg tcgagggagc cgtccgtcga cgtcacgtcc gtgcgcctgc
      421 gtcgtcagct cgccggcttc tccgggagcc gctcgatcgc ggccagcacc gcgtcgaggt
      481 cgggcggctc caggcgcagg tcgaagtggc gggcgagcgc atcctcgaac tcggcgcgcg
      541 tcgcgagcgt gcgcagctgc ggcgcggcgg tcatcggctg gcgcagcgac agctcgcggt
      601 tgaacagcgt ccagcgcccg ccgtcgaccg cgcggccgac cagcaggttc gcgcccagga
      661 aggactcggg atgcgtcgcg acgaaccagt tggccgcctt gtagtcgatc gccgccggcc
      721 gcgagggcag gatcaggcag gcgtcgcccc agccctcgtg cgacaggctt tgcatcatca
      781 ggccggtttc gacgtcgacg aagcggaagc gcccggtgcc tgcggcctgc tcgccgcggt
      841 cggccaggcg cagcggggcg gccggcgcgg tgctgccgaa gccgacgtcg acgtgccagt
      901 cctcgcccgc aagcgtcacg cgcagcgtca tgtgcgtgcg cgcggactcg cgctcggccg
      961 gcacgccgac gcgcacgcgc gcctcctggc cgcgcgcgtc gaagccgagg cggcgcagca
     1021 ccgcgagcat caggtggttc tgctcgaagc agtagccgcc gcggcgtcga cgcaccagct
     1081 tgtcttccag cgccgcggtc gacagctcgg gcacgcggcg cgcgagcacg tccacgttct
     1141 cgaacgggat cgagtgcgcg tggcgatcca ccaggcgcgc gagcgtctcc agcgtcggcg
     1201 cggtcgggcc ggcgtagtcg atgcggtgga agtaggcctc gaggtcggcc agcggatcgg
     1261 cggccggttg gagcatcgtc atcggggatc cctggacttg cgggcgcgca cgcgctgctc
     1321 gcgctcgacc tcgcgttcgg cttcgcgctc ggcctcggcc gcggcggcct cggcctccca
     1381 ggcgaggaac tgcgcgggcg actcgagcgt cacgcggccc caggcgccgg cgcggaagtc
     1441 gttgagcgcg acctccgccg ccttggtgcg gttgatgcgg ccgccggcca tcagcgcgcc
     1501 gcgcttgcgg ccgatggcct ccagcagctc cccgtcggcc agcggggcga tgtcgtcgag
     1561 cttgtagcgc gcgcgcagcc ggtcgtcgta gcgcccgcgc acggaagcga gcagctccag
     1621 cgcgacctcc tcctcgtcgt acgcgttgcg gcccaccgcg ccgctggcgg ccaggtgata
     1681 gccggccttc tcgacgccga tgcgcggcca cagcatgccg ggcgtgtcga acaggtagac
     1741 gtcgctcgcg agcgtgatgc gctgctccac cttggtgatg ccggcctcgt cgcccgtctt
     1801 ggcgacgcgc tggccggtga tcgtgttgat gagcgtggac ttgccgacgt tgggaacgcc
     1861 gcataccagc acccgtatcg gcttgaccat gccgccgcgc tgcggcacga gctcgcggct
     1921 cgcctcgacc agccggcgcg cgggcgcggg gacgctcgcg tccagcccga tcgcgcgcgt
     1981 gccgggcagg gcgccgaagt gggcgagcca ttgcgcggtg gcgtccgcat ccgcgaggtc
     2041 ctgcttgttg aggatcttca gggccggctt gccgcgcgtc atgtcggcga gcagcgggtt
     2101 gccgctgctg ccgggcaggc gggcgtcgag cagctcgatc acgacgtcga tctcgggcag
     2161 gcgctccgcg atcgccttgc gcgtcgcgtg catgtggccg gggaaccact ggatgggcat
     2221 ggatcgtcgt cgggcggaag gctgcggaag ggacgattgt ccgtccaatc gggcatgccc
     2281 ggcggcatcc ggcgggatga ccgggcgccg aggccgccgc ggcggcgctg tcggcctcgt
     2341 cccacaacgc gacgcgccgc ctgccgaatg cgcggccggc cgggcgatgg tcagatggcg
     2401 ggcatcgcgg gaatcgcctc gccctggagg atcgccatgc tcaagtgggc cctcgtcttc
     2461 gccctcgtct cgctcgtcgc cggcctgctc ggcttcaccg gactggccgc cggagcggcc
     2521 gccatcgcca agaccctgtt cgtcgtcttc gcggtgctgt tcctgctgtt cctggtgctg
     2581 ggcgccacga tcttccgcaa gatcacggac taggcggtgg gcccagcccg gggcgcgtgg
     2641 cgcgccccgc ccgcccgcgt cagtcgaacc gcagggacac gcccgtcacg agctgcgtgt
     2701 cgttgcggcc caggccctcg gccgggtggc tgtcgtagcg gtgctcgaag gtcgcggtca
     2761 gcgcgaggcg cttggtcatc gccaccgaca ggccggcgtc gaacagcgtg cgcacgtcgt
     2821 cgctgttgcg caggctcggg tagacggtga gcttctggtg gaaagtcgtc gtgtcggtga
     2881 acttgtggtt cgactcctcg gcgaccagca ggtcgaggtg gttgtagcgg ctgcgcagct
     2941 cgtcgtcgac gaccacgggc ggcgcgtagc gatccatcgt gtagcccagc cccgcggaca
     3001 ggtcccacgt gagcttgtcc tccctgatcg cgtggaagcc gacgccggtg ccgagcgtcg
     3061 tgcgcgagcg gatgtcggca agcgggtcgt gcagcaggtc gagctggccg aagccgaacc
     3121 agcgcggcgt gaagtcgctc gagtacagcg ccgtgccgct ctggcggttg gcggtgcgcg
     3181 cgccgtcgct ggaggcgtac tggccgttgg catcgaaggc ccacttgtcg tggtccgtca
     3241 ggcggatgac atcggcctgc agcgaggcgg tgtcggtgtg cgtgttgccg cgcgcgacgg
     3301 tcgcgcccag cgtgaacagc gagcgccact ggccgtccgg cttctgcgtc acctgcgcat
     3361 gcgcgagcag cggcgcggcg gcggcgaggg cggcgaggac ggaacgggcg gacgggcgga
     3421 tctgcatcgg aagggctcct gggttgagat tccccgggcg ccggcagttc tcgcgcccgg
     3481 gccagctcgt cggtgcgccg aatttgtgtc gaatggcacc ccgcaccgcg cagcgagcgc
     3541 cgcgcactac gtccgcacga cttcgagccc tgcgacaagc tcgacatcgt cgactacggc
     3601 gactgcgagg cgcatgcccg ctgccttcct cgagcaaggc gtgcaggtgc gggcgatcgg
     3661 cggcgaccac gtcatcggct atccgctgct gcgggcgcat gtccggcgca cgtcgcggcg
     3721 gagatgctct gcctgatggc ctgagcgctg cggcggcatc cgttcggcgt gtgatcgggc
     3781 cggcgtgccg gaacggcatt tgctgtgtcg ggccgccctt tcacaggggc ttcaccgctt
     3841 gttacggatg cgccggccgc cggcgtcccg ggcgcggccg gccggcctac catggtccgc
     3901 gatcggtgcc ggcgcgacag gacgcgccgg cccgcgacgg agattcgatc gtgacccagg
     3961 atgaccagcg cacgtgcccg ggctgcca
//
LOCUS       DASVLN010000021         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_54809, whole genome shotgun sequence.
ACCESSION   DASVLN010000021 DASVLN010000000
VERSION     DASVLN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54809"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VF453_00505"
     CDS             complement(<1..288)
                     /locus_tag="VF453_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686146.1"
                     /translation="MDAAGSDARIAAVRTELAAVVASYRALVGAASKASGASLPRIDA
                     ALAGFEPGFFNNLLVALDARFEREAGEPAPVAEVRLLADAVKARGGTLAVDP"
     gene            complement(296..742)
                     /locus_tag="VF453_00510"
     CDS             complement(296..742)
                     /locus_tag="VF453_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686147.1"
                     /translation="MKHLFSLTAGLLLATLAAHAAADAPPAGVWIGSGASYRHDFAFD
                     FHPDGSLRLLGRVTDEMTARRARMPADERAAFDAHTTTLAGGLWLAMPGTWRMLDDGR
                     VHLHGDPPPGRDKAFDMDFALRVVDARHVKLRTEFGIEQTLEPLEP"
     gene            complement(854..>1738)
                     /locus_tag="VF453_00515"
     CDS             complement(854..>1738)
                     /locus_tag="VF453_00515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7686148.1"
                     /translation="WIVAYPAAGGSDFLARQLAPQLGKQLGQTLVVDNRPGAAGIIGT
                     EAASKAPGDGYTILTGDNGAMVFHTALYKSLPYNPADFVPIGFMASFPLILVVNPASG
                     FTSARQLLDAVKRSPGKYSYASPGAGSPHHLAMELLKERTGSFIVHVPYRGTAPAMQD
                     VMSGQVPMMIVDTAAGLPQIRGGKVKALAVLSPHRIPQLPDVPTLSEIGADRGLKDFD
                     ANAWQGLFAPKGTPPEIVQRLGVEMRKAILAPEVRRRLEEFGLEVAPTDGPQLAAFIQ
                     KETVFWHKLIKDRHLSLD"
BASE COUNT          238 a          598 c          666 g          236 t
ORIGIN      
        1 cggatcgacg gccagcgtgc ccccgcgcgc cttcacggcg tcggcgagca ggcgcacctc
       61 ggcgaccggg gccggctccc cggcctcgcg ctcgaagcgc gcgtccagcg ccaccagcag
      121 gttgttgaag aagcccggct cgaagcccgc gagcgccgcg tcgatgcgcg gcaggctcgc
      181 gcccgaggcc ttggacgcgg cgccgaccag cgcgcggtag ctggcgacca cggccgcgag
      241 ctcggtgcgg acggcggcga tgcgggcgtc ggatccggca gcgtccatcg cgcgttcagg
      301 gctcgagggg ctcgagcgtc tgctcgatgc cgaattcggt gcgcagcttc acgtgccgcg
      361 cgtcgacgac gcgtaacgcg aagtccatgt cgaaggcctt gtcgcggcca ggcggggggt
      421 cgccatgcag gtggacacgg ccgtcgtcga gcatccgcca ggtcccgggc atcgccagcc
      481 agagcccgcc cgcgagcgtc gtcgtgtggg cgtcgaaggc cgcgcgctcg tcggcgggca
      541 tccgggcgcg ccgcgccgtc atctcgtcgg tcacccgccc gagcaggcgc agcgagccgt
      601 ccggatgaaa gtcgaaggcg aagtcgtgcc gataggacgc gccgctgccg atccagacac
      661 cggcaggcgg cgcgtcggcc gcggcatgcg cggcgagggt tgccagcagc aggccggcgg
      721 tcaggctgaa gaggtgcttc atgcggttgc ggccacggtc gggcgatcaa ggcgacgacc
      781 gcatccccgg cggcgagccg cgtcggcacg gaaggccgcg caccgcggcc agacggttgg
      841 aaagaccacc acgtcagtcc agcgacaggt gccgatcctt gatcagcttg tgccagaaca
      901 ccgtctcctt ctggatgaag gcggctagct gcgggccgtc ggtgggcgcg acctccaggc
      961 cgaactcctc caggcgccgc ctgacctcgg gcgcgaggat ggccttgcgc atctcgacgc
     1021 cgaggcgctg gacgatctcg ggcggcgtgc ccttgggcgc gaacaggccc tgccaggcgt
     1081 tggcgtcgaa gtccttcagg ccgcggtcgg cgccgatctc ggacagcgtg ggcacgtcgg
     1141 gcagctgcgg gatgcggtgc ggcgacagca ccgccagcgc cttgaccttg ccgccgcgga
     1201 tctgcggcag cccggcggcg gtgtcgacga tcatcatcgg cacctggccg ctcatcacgt
     1261 cctgcatcgc cggcgcggtg ccgcggtagg gcacgtggac gatgaagctg ccggtgcgct
     1321 ccttgagcag ctccatcgcg aggtggtggg ggctgcccgc gccgggcgac gcgtagctgt
     1381 acttgccggg gctgcgcttg accgcgtcga gcagctggcg cgcgctcgtg aagccgctgg
     1441 ccgggttgac gacgaggatc agcgggaagc tcgccatgaa gccgatcggc acgaagtccg
     1501 ccgggttgta cggcagcgac ttgtacagcg cggtgtggaa caccatcgcg ccgttgtcgc
     1561 cggtcaggat cgtgtagccg tcgccgggcg ccttggacgc cgcctcggtg ccgatgatgc
     1621 cggccgcgcc ggggcggttg tcgacgacga gggtctggcc cagctgcttg cccagctgcg
     1681 gcgcgagctg gcgggcgagg aagtccgagc cgccggccgc cgggtaggcg acgatcca
//
LOCUS       DASVLN010000022         7837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_270104, whole genome shotgun sequence.
ACCESSION   DASVLN010000022 DASVLN010000000
VERSION     DASVLN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270104"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..894
                     /locus_tag="VF453_00520"
     CDS             52..894
                     /locus_tag="VF453_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX7686149.1"
                     /translation="MRRPSLATLLMLALALTCARAPAQPPGAAAAASASVSTSADYVL
                     GPGDRIRVTVFQSPELSIDTRIPESGVVAYPLVGDLALGGKTLSQAEKRVAEGLVAAR
                     LVRVPQVAIVVTEIHANQAAVLGFVVHPGRYALEARSMRLSGLIALAGGPTPEASDIL
                     TITGTRDGKPMRLAVDTRRLLTGADREHDIEIRNGDIVYLERAPQVYVHGEVQHPGTL
                     RLEPGMRVLQAIAAAGGVTMRGTKRGVHVSRPGPDGALVDSTPGLQAVLEPDDVVYVP
                     ESLF"
     gene            912..2345
                     /locus_tag="VF453_00525"
     CDS             912..2345
                     /locus_tag="VF453_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEX7686150.1"
                     /translation="MNLAHFLAVARARWRLALAVFLVTVAAAVVAALVVPRTYTATAA
                     LLIDVSRPDPVTGLSNGGNPSPTILATQVGVLKSERVAREVVRRLDLASDAKLRAAWM
                     ASTQGRGDFTGWLAQALQKKTEILPERDANVVAIDAQAGTPEQAARIADTFAQVYLDV
                     SAAIRAEAAQQASAFFERRSTELRADLERAQARLADYQRTNGVVVSDAQVDSETTRLN
                     ELSLQLAVAQAAAAETGSRSAFAGQGGADQLPEAQSSLVVSGLRADLVRAETQLQELT
                     SRLDVNHPQVVQARNAVALLRNRLDAETRRIGSAVTLASAAQRQREAELRGAMEAQRQ
                     RVLRLKTLQGDGHVLLRDVENAQRAYDGVEARLSQAALESHVMQGTATLLAPAMVPAA
                     PSSPGRTTTVALAGVLGLVLAIAAVLLLEFADPRLRTEAAAAQALGVQVLGAVPRPDT
                     AGSFRSRKVPLVGRPGVRRLAAPSQES"
     gene            2348..3259
                     /locus_tag="VF453_00530"
     CDS             2348..3259
                     /locus_tag="VF453_00530"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX7686151.1"
                     /translation="MNNDDVAVLGAGFDLPLATMGLPTLGPARRLGEILGERIDLAPA
                     RVVEILAHAGKHGMRFGDAAVALGYATPEQVVLALSVQFQYPCVVDTPTVPSAELPLL
                     TRPHGAQAEALRALRGRVSRRMARLGEERRAVAVVSPEPGDGKTFLVANLGVALAQTG
                     ARTLLVDADMRGPRLHEIFELRQRHGLSGAVIGCADAQIIQPVAIVPGLHLLACGATP
                     PNPLELIERDAFRSLVRHLPAHFDFVLVDTPAAAYGADALAIADCCGASLLVGRRDRS
                     GMAALRRLADDLSDGDGVLLGAIVNDF"
     gene            3281..4486
                     /locus_tag="VF453_00535"
     CDS             3281..4486
                     /locus_tag="VF453_00535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7686152.1"
                     /translation="MSRAPEPSDPVTRHLADPRICFVGLQNLPVLSPRHREHGIGGEE
                     VQQTLLARALARRGFEVSMVVADYGQADGEVIDGIRLFKAGGLDEGLPGLRFVHPRMT
                     RLWAALARADADVYYVSIAGPQLGVVAAFARLHRRRVVFRIASDADCDPARLLVREAR
                     NRRLYAFGLRRADVVLAQTARQALELERHYRLPSRLAGMLVEPARRALPFAERDIDVL
                     WVANLQPLKRPQLVLQLARRLPGLRVHLAGGEQAGHASLYREVADAAAALPNVRFHGR
                     VPYHEVSGLFERARVLVNSSDIEGFPNTYLQAWRHGVPVVAFFDPDHVVARFGLGAAV
                     PSIDAMAAEVSRLAGSQADWAPASARCERYMDDLYGEERVLAPYLQALAPAHGTWPFE
                     RSALLETSS"
     gene            4483..5532
                     /locus_tag="VF453_00540"
     CDS             4483..5532
                     /locus_tag="VF453_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacetylase"
                     /protein_id="HEX7686153.1"
                     /translation="MSLPAFLVTIDTEGDDLWSRPRDITTRNVASLPRFQALCERHGL
                     RPTWLVDYEMAMDPAFVRFGREVLRRDAGEIGMHLHAWNTPPLTPLTRDDFLHQPFLT
                     DYPDDVAEEKVVVVTELLRDRFETRIASHRAGRWAFDARYARTLARLGYLVDCSVCPH
                     VSWAATLGDPAGRGGPDFRRFPDRPYMLDLDHIDRPGTSDLLEVPVSVLRSRLHRLAP
                     WSYRTPLLRRYAWRAAPDRHWFYPDGRNLASLRAIVAEALRSGRPCVEMILHSSELMA
                     GGSPNGADAAAVQRLYDDLEEVFAVASRQFRGMTLSEFRHAWVARSFAASAPQARPQP
                     APLRPTPSASRGDAS"
     gene            5529..6653
                     /locus_tag="VF453_00545"
     CDS             5529..6653
                     /locus_tag="VF453_00545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7686154.1"
                     /translation="MTLPHICFIAVHIYPVLVQGTGLGFVGGAEVQQAVQMRALRRAG
                     YRISVLAKDDGQPDVVEVDGITIHKIPSDAGRGLRGTRFLYPRLTDVVGLLRRVAPDV
                     VFVQAAGEQLAAAALYARTAGKRLVFAGASDPDFARGVLPGMSRWHSTLYRLGLRGAH
                     EILVQNQAQRDALARNFGRRGRLVQNGYDERGTQPASFEGHVLWVATIKPLKRPDLFI
                     ELARRLPGRRCVMVGGAGLAPDAQRFLESVQHSAAGVANLELTGHVPFDQVGRWFDGA
                     ALVINTSDYEGLPNTFMQAWIRGTPTLSFVRPESSPGVSGTIACDDIEDMAAKARVLT
                     TDRGAWARASAACRRHFEAHHTLERAVAHYAEAFAAAVPA"
     gene            6650..>7837
                     /locus_tag="VF453_00550"
     CDS             6650..>7837
                     /locus_tag="VF453_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686155.1"
                     /translation="MMADRLDTAGVPAGERRMGRVMVAALGWGGGMAAVRMACSFVSI
                     KITAVALGPAGLALVAQFSNFVSLFQSMLGQALTTGVVRLDAEYAQDPPRRLRVRATA
                     ARMLVALAVVFGLVMAVASAPVAGWLLSDRAYAVLIALSGIAVAAAMATDLLNGTLSA
                     VQEIGLVGRATMASTILGLAVFAPCAYLWGVAGGLWGSFAVLVATAGVSAIVVRRRSR
                     GVRLGDFRGAFDRTECRRLLGFYPMLLVNGALAPLTLILVRDALVEHLGLDAAGLWQA
                     SWRLSEAYQAVILSSTTLYFMPSMGARIGDPRALRRQLLRTLALATGSTAALAAGIAI
                     LRVPIVHAVFSARFAPVAGLLPLQLAGDVLKMAGWILAMALVAAMRTRRFIALAVISA
                     AGFA"
BASE COUNT          909 a         3029 c         2824 g         1075 t
ORIGIN      
        1 atcgcgccgt cgatgcccgc gcgggcgggc gcctgaacac cggagaccgt catgcgccgc
       61 ccatcccttg ccaccctcct catgctggcg ctggcactga cctgcgcgcg cgcgccggcg
      121 cagccgcccg gggccgccgc cgcggcctcg gcctcggtgt cgacgtccgc cgactacgtg
      181 ctcggcccgg gcgaccgcat ccgcgtgacg gtcttccaga gccccgagct gtcgatcgac
      241 acccgcatcc ccgagagcgg ggtcgtcgcc tacccgctcg tcggcgacct ggccctcggc
      301 ggcaagacgc tgagccaggc cgagaagcgc gtcgccgaag gtctcgtcgc cgcgcggctg
      361 gtgcgcgtcc cgcaggtcgc gatcgtcgtg accgagatcc acgcgaacca ggcggccgtc
      421 ctcggcttcg tcgtgcaccc gggccgctat gcgctggagg cgcgctcgat gcggctgtcc
      481 gggctgatcg cgctggccgg cgggccgacg cccgaggcgt ccgacatcct caccatcacc
      541 ggtacgcgcg acggcaagcc gatgcgcctg gccgtcgaca cccggcgcct gctgacgggc
      601 gcggatcgcg agcacgacat cgagatccgc aacggcgaca tcgtctacct cgagcgcgcg
      661 ccgcaggtct acgtccacgg cgaggtgcag caccccggca cgctgcgcct ggagccgggc
      721 atgcgcgtgc tgcaggccat cgccgcggcc ggcggcgtga cgatgcgcgg cacgaagcgc
      781 ggcgtgcacg tgagccgccc ggggccggac ggcgcgctcg tcgactcgac gcccggcctg
      841 caggcggtgc tggagcccga cgacgtcgtc tacgtgcccg agagcctctt ctgacgccgc
      901 ggagcccgca gatgaacctc gcccacttcc tcgccgtcgc ccgcgcgcgc tggcggctcg
      961 cgctggccgt gttcctcgtg acggtggccg cggcggtcgt cgccgcgctg gtggtgccgc
     1021 gcacctacac cgcgaccgcg gcgctgctga tcgacgtgtc gcgccccgac ccggtgaccg
     1081 gcctgtcgaa tggcggcaac ccgtcgccga ccatcctcgc gacgcaggtc ggggtgctca
     1141 agagcgagcg cgtcgcgcgc gaggtcgtcc gccggctgga cctcgcctcc gacgccaagc
     1201 tgcgcgccgc ctggatggcc agcacccagg gccgcggcga cttcacgggc tggctcgcgc
     1261 aggcgctgca gaagaagacc gagatcctgc ccgagcgcga cgccaacgtg gtggcgatcg
     1321 acgcgcaggc gggcacgccc gaacaggcgg cgcgcatcgc ggacacgttc gcgcaggtct
     1381 acctcgacgt ctcggcggcg atccgcgccg aagcggcgca gcaggcgtcg gcgttcttcg
     1441 agcggcgctc gaccgagctg cgcgcggacc tcgagcgcgc gcaggcccgc ctcgcggact
     1501 accagcgcac caacggcgtg gtggtgagcg acgcccaggt cgacagcgag accacgcggc
     1561 tcaacgaact ctcgctgcag ctcgccgtcg cgcaggccgc ggccgccgag accggcagcc
     1621 gcagcgcctt cgccggccag ggcggcgccg accagctgcc cgaggcgcag tccagcctgg
     1681 tcgtctccgg cctgcgcgcc gacctcgtgc gcgccgagac gcagttgcag gagctcacct
     1741 cgcggctgga cgtgaaccac ccgcaggtgg tgcaggcccg caacgccgtg gcgctgctgc
     1801 gcaaccgcct ggacgccgag acgcgccgca tcggcagcgc cgtcaccctg gcgagcgccg
     1861 cgcagcgcca gcgcgaggcc gagctgcgcg gcgcgatgga ggcgcagcgc cagcgcgtcc
     1921 tgcgcctgaa gacgctgcag ggcgacggcc acgtgctgct gcgcgacgtc gagaacgcgc
     1981 agcgcgccta cgacggcgtc gaggcgcgcc tgagccaggc ggcgctggag agccacgtga
     2041 tgcagggcac cgccacgctg ctcgcgcccg cgatggttcc cgcggcgccg tcgtcgccgg
     2101 gccgcacgac gacggtcgcc ctcgccggcg tgctcggcct ggtgctggcg atcgcggcgg
     2161 tgctgctcct ggagttcgcc gacccgcgcc tgcgcaccga ggccgccgcc gcgcaggccc
     2221 tgggcgtgca ggtgctgggt gccgtgccgc ggcccgacac cgcgggatcg ttccgctcgc
     2281 gcaaggtgcc cctggtgggc cggccgggcg tccgccgcct cgcggctccg tcccaggaat
     2341 cctgacgatg aacaacgacg atgtcgccgt gctcggtgcc ggcttcgacc tcccgctcgc
     2401 gacgatggga ctgcccacgc tcgggccggc gcgccggctg ggcgagatcc tcggcgagcg
     2461 catcgacctg gcgcccgcgc gggtggtgga gatcctcgcc cacgccggca agcacggcat
     2521 gcgcttcggc gacgccgcgg tcgcgctcgg ctacgccacc ccggagcagg tcgtgctcgc
     2581 gctgtcggtg cagttccagt acccgtgcgt ggtcgacacg cccacggtgc cgagcgcgga
     2641 gctgccgctg ctgacgcgtc cccacggcgc gcaggccgag gcgctgcgcg cgctgcgcgg
     2701 tcgcgtgtcc cggcgcatgg cgcgactggg cgaggagcgc cgcgccgtcg ccgtcgtcag
     2761 ccccgagccg ggcgacggca agaccttcct cgtggccaac ctgggcgtcg cgctggcgca
     2821 gaccggggcg cgcacgttgc tggtcgacgc cgacatgcgc gggccgcggc tgcacgagat
     2881 cttcgagctg cgccagcgcc acggcctgtc gggcgcggtg atcggctgcg ccgacgcgca
     2941 gatcatccag ccggtggcca tcgtcccggg gctgcacctg ctggcgtgcg gcgccacgcc
     3001 gcccaacccg ctggagctca tcgagcgcga cgccttccgc agcctcgtgc ggcacctgcc
     3061 ggcccatttc gacttcgtgc tcgtggacac gccggccgct gcctatggcg cggacgcgct
     3121 ggcgatcgcc gactgctgcg gcgcgagcct cctggtcggt cgccgcgacc gctccggcat
     3181 ggccgcgctg cgacgcctgg ccgacgacct gtccgacggc gacggcgtgc tgctgggcgc
     3241 catcgtcaac gacttctgac cgcctcccga ggaatcgccg atgagccgag cgcccgagcc
     3301 gtccgacccc gtcacgcgcc acctggccga tccgcggatc tgcttcgtcg gcctgcagaa
     3361 cctgcccgtt ctctcgccgc gccaccgcga gcacggcatc ggcggcgagg aggtgcagca
     3421 gaccctgctc gcgcgcgcgc tggcgcgccg cggattcgag gtctcgatgg tggtcgcgga
     3481 ctacggccag gccgacggcg aggtcatcga cggcatccgc ctgttcaagg ccggcgggct
     3541 ggacgagggc ctgcccgggc tgcgcttcgt ccatccgcgc atgacgcggc tgtgggcggc
     3601 gctcgcgcgc gcggacgccg acgtctacta cgtcagcatc gccggtccgc agctgggcgt
     3661 cgtcgccgcc ttcgcgcgcc tgcaccggcg ccgcgtcgtg ttccgcatcg cgagcgacgc
     3721 cgactgcgac cccgcgcgcc tgctggtgcg cgaggcgcgc aaccgccggc tctacgcgtt
     3781 cggcctgcgg cgcgcggacg tggtgctcgc gcagaccgcg cgccaggcac tggagctcga
     3841 gcgccactac cgcctgccgt cgcgcctggc ggggatgctg gtcgagccgg cgcggcgcgc
     3901 gctgcccttc gccgagcgcg acatcgacgt cctctgggtg gcgaacctgc aacccctgaa
     3961 gcggccgcag ctcgtcctcc agctggcgcg gcgcctgccg gggctgcgcg tgcatctcgc
     4021 gggcggcgag caggccggcc atgcgtcgct gtaccgcgag gtcgccgacg ccgcggccgc
     4081 gctgcccaac gtgcgcttcc acgggcgcgt gccgtaccac gaggtctcgg gcctgttcga
     4141 gcgcgcgcgc gtgctcgtca acagctcgga catcgagggc ttccccaaca cctacctgca
     4201 ggcctggcgg cacggcgtcc cggtggtcgc cttcttcgac ccggaccacg tcgtcgcgcg
     4261 cttcggcctc ggggcggccg tgccctcgat cgacgcgatg gcggccgagg tgtcccggct
     4321 cgcgggctcg caggccgact gggcgcccgc ctccgcgcgc tgcgagcgct acatggacga
     4381 cctctacggc gaggagcgcg tgctcgcgcc ctacctgcag gccctggcgc ccgcccacgg
     4441 cacctggccg ttcgagcggt cggccctcct ggagacatca tcttgagcct gcccgccttc
     4501 ctcgtgacca tcgacaccga gggcgacgac ctctggtcgc gcccgcgcga catcacgacg
     4561 cgcaacgtcg ccagcctgcc gcgcttccag gccctgtgcg agcgccacgg cctgcggccg
     4621 acgtggctcg tggactacga gatggcgatg gatccggcgt tcgtccgctt cggccgcgag
     4681 gtgctgcggc gcgacgccgg cgagatcggc atgcacctgc acgcctggaa cacgccgccg
     4741 ttgacgccgc tgacgcgcga cgacttcctg caccagccct tcctcacgga ctatccggac
     4801 gacgtcgcgg aagagaaggt cgtggtggtc accgagctgc tgcgcgaccg cttcgagacc
     4861 cgcatcgcca gccaccgcgc cggccgctgg gccttcgacg cccgctacgc ccgcacgctg
     4921 gcccgcctgg gctacctcgt ggactgctcg gtctgcccgc acgtgtcctg ggccgccacc
     4981 ctgggcgatc ccgccggccg tggcggcccc gacttccgcc gcttccccga ccggccgtac
     5041 atgctggacc tggaccacat cgaccgcccc ggcacctcgg atctgctgga ggtgccggtg
     5101 tcggtgctgc gctcgcgcct gcaccggctg gcgccgtggt cgtaccggac gccgctgctg
     5161 cgccgctacg cctggcgcgc ggcgccggac cgacactggt tctaccccga cggccgcaac
     5221 ctcgcgagcc tgcgcgcgat cgtcgccgag gcgttgcgca gcggccggcc ctgcgtcgag
     5281 atgatcctgc actcgtcgga actgatggcc ggcggcagtc ccaacggtgc cgatgcggcg
     5341 gccgtgcagc gcctgtacga cgacctcgag gaagtcttcg cggtggcctc gcggcaattc
     5401 cgcggcatga cgctgtcgga gttccgccac gcctgggtcg cgcgcagctt cgccgccagc
     5461 gcgccgcagg cccgaccgca gccggcgccg ctgcgcccca cgccttccgc gagccgggga
     5521 gacgcctcgt gacgctgccc cacatctgct tcatcgcggt ccacatctac cctgtcctcg
     5581 tgcagggcac gggcctgggc ttcgtcggcg gcgcggaggt gcagcaggcc gtgcagatgc
     5641 gcgcactgcg gcgcgccggc taccgcatct ccgtgctggc caaggacgac ggccagcccg
     5701 acgtcgtcga ggtggacggc atcacgatcc acaagatccc ctccgatgcc ggccgcggcc
     5761 tgcgcggcac gcgcttcctc tacccgcgcc tgacggacgt cgtgggcctg ctgcggcggg
     5821 tcgcgccgga cgtcgtgttc gtgcaggctg ccggcgagca gctcgccgcc gcggcgctgt
     5881 acgcgcgcac ggcgggcaag cggctcgtct tcgccggcgc gagcgatccg gacttcgccc
     5941 ggggcgtcct gcccggcatg tcgcgctggc acagcacgct ctaccggctc gggctgcgcg
     6001 gcgcccatga gatcctggtg cagaaccagg cgcagcgcga cgcgctcgca cgcaacttcg
     6061 ggcgccgcgg ccggctggtg cagaacggct acgacgagcg cggcacgcag cccgcgtcgt
     6121 tcgagggcca cgtcctgtgg gtcgcgacca tcaagccgct caagcgcccg gacctgttca
     6181 tcgagctcgc gcgccgcctg ccgggacgcc gctgcgtgat ggtcggcggc gcgggtctcg
     6241 cgccggatgc gcagcgcttc ctcgagtcgg tgcagcactc ggccgccggc gtggccaacc
     6301 tggagctcac cggccacgtg cccttcgacc aggtcggccg ctggttcgac ggcgcggcgc
     6361 tcgtgatcaa cacctcggac tacgagggcc tgcccaacac cttcatgcag gcctggatcc
     6421 gcggcacgcc cacgctgtcg ttcgtgcggc cggagtcgag cccgggcgtc agcggcacca
     6481 tcgcgtgcga cgacatcgag gacatggcgg ccaaggcgcg cgtgctgacg accgaccgtg
     6541 gcgcgtgggc gcgcgcgagc gcggcctgcc gccggcactt cgaggcccac cacaccctcg
     6601 agcgcgccgt cgcccactac gccgaggcct tcgccgcggc cgtcccggca tgatggcgga
     6661 tcgcctggac acggccggcg tccccgccgg cgagcgacgc atggggcgcg tcatggtcgc
     6721 ggcgctgggc tggggcggcg gcatggcggc cgtgcgcatg gcctgcagct tcgtcagcat
     6781 caagatcacg gcggtggcgc tcgggcccgc cggcctcgcg ctggtggcgc agttctccaa
     6841 cttcgtgagc ctgttccaga gcatgctcgg ccaggcgctg accaccggcg tcgtgcgcct
     6901 ggacgccgaa tacgcgcagg atccgccgcg ccgcctgcgg gtgcgcgcga ccgcggcgcg
     6961 gatgctggtc gcgctggcgg tggtgttcgg cctcgtcatg gccgtcgcca gcgccccggt
     7021 ggccggctgg ctcctcagcg accgcgccta cgccgtcctc atcgcgctca gcggcatcgc
     7081 cgtggccgcg gccatggcca ccgacctgct caacggcacg ctcagcgcgg tgcaggagat
     7141 cggcctggtc ggccgcgcga ccatggcctc gacgatcctg ggcctcgccg tcttcgcgcc
     7201 gtgcgcctac ctctggggcg tcgccggcgg gctgtggggc agcttcgcgg tcctcgtggc
     7261 cacggcgggg gtctccgcga tcgtggtcag gcgccggtcg cgcggcgtgc ggctcggcga
     7321 cttccgcggc gccttcgacc gcacggaatg ccggcgcctg ctcggcttct atccgatgct
     7381 gctggtcaac ggcgcgctcg cgccgctgac tctgatcctc gtgcgcgacg ccctggtcga
     7441 acacctgggc ctggacgccg ccgggctgtg gcaggcgagc tggcggctgt ccgaggccta
     7501 ccaggccgtg atcctgtcgt cgacgacgct gtacttcatg cccagcatgg gcgcgcgcat
     7561 cggcgacccg cgggcgttgc ggcgccagct gctgcgcacg ctggccctgg cgaccggctc
     7621 caccgcggcg ctcgcggcgg gcatcgcgat cctgcgcgtg cccatcgtgc acgcggtctt
     7681 cagtgcgcgc ttcgcgcccg tggccggcct gctgccgctg cagctggccg gcgacgtgct
     7741 caagatggcg ggctggatcc tcgcgatggc gctcgtcgcg gcgatgcgca cgcggcgctt
     7801 catcgcgctg gcggtgatct cggcggcggg cttcgcc
//
LOCUS       DASVLN010000023        27037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_377134, whole genome shotgun sequence.
ACCESSION   DASVLN010000023 DASVLN010000000
VERSION     DASVLN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 27037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377134"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VF453_00555"
     CDS             <1..396
                     /locus_tag="VF453_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX7686156.1"
                     /translation="GPGLGGQIAGTGGHGLGTGSGRAGGTLTKGGGGKASRSLEEVRL
                     VLERTKPALYAIYNRALREDPTLQGKVVLELTIAPNGSVTEAHFKSSELKSPELEQKL
                     LARFRLMEFKAEDVETLVTTYPIDFLPSG"
     gene            complement(411..3245)
                     /locus_tag="VF453_00560"
     CDS             complement(411..3245)
                     /locus_tag="VF453_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7686157.1"
                     /translation="MNRRPASPPPADSRPPRPTLATWLFVSVLGATMVAVLTCTLVIE
                     SFVRQEERRQATQFLQSDADELRDALDRGMAQNFEDVRVLGRLDQVADGNDPAAVRRA
                     LEAMRGSFPQFAWMGLAGTDGKVIASVDGLLQGVDVSARPWFAGARHGMYVGDVHAAA
                     LLAGLLPRQAEPWRFVDIATPVYRADGSLRGILAGHLSWQWAAQIERDLVDRELAQHQ
                     AEALVVGADGVVLLGPPALQGRKLPAFDAAREFAVESRTQGSGRYPGLGWRVVLHQPE
                     PVAMATFHALQLRTRLAAALLCLVLAPLAWLLARRLATPLRALEAELDVVDAAPPARG
                     LPLYREAELLGHALDRYARRQREDAAHLRDLNAGLEARVAERTAALERLNGELSQAVR
                     ERWQSEERLRAILTHAPDAYVAIDEAGRISEWNRRAEQTFGWTRAEAIGQMVDDLLTP
                     PGLRDEWRTGVDAFTHGRGASAGHRVERAVLHKSGAEIPVQLSAAALRTDGGLVAYAF
                     MHDISERKRAERRLAQSERRLRTVTDNLPALVSHIDTGLRYTFTNATYGRWFDVDHAA
                     MVGRGVADVVGAEAFAGLDGAMRRALAGEASSSEREVVILGRRMHALIHYVPDLDEDG
                     RVQGIYGMVLDLTARRDVELALARSEQRLRTIADNLPVLISYVDRDERYQFANGTYRE
                     WIGQAPAQIVGRTMADVLGPEVHGARRALVRRALAGEHVEFDIETSMMGIRRHLHTLY
                     LPDTGADGTTAGFYVLVSDVSAMKLAEKRLALQARTDALTGLPNRYAFNEKLAEALAR
                     SRRNEQAVGLFFLDIDRFKSINDTLGHAAGDQVLSVFAQRLRASVREVDAVARLAGDE
                     FVVILEGLHTPFEPQAIARKILAAMAQPLAVEGRELLITASIGIAYQADGRAQADELL
                     ARADEALYAAKAAGRGTFRMAGAAPVDARA"
     gene            complement(3339..3482)
                     /locus_tag="VF453_00565"
     CDS             complement(3339..3482)
                     /locus_tag="VF453_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686158.1"
                     /translation="MRGIGHGKEGVPYVPGRFEDFLLAGRSMRADVHGWLAARLPKRS
                     RLR"
     gene            complement(4170..4334)
                     /locus_tag="VF453_00570"
     CDS             complement(4170..4334)
                     /locus_tag="VF453_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686159.1"
                     /translation="MNRLQSLRYHRPVAGTRAFAPALQSMSLKAGADAGGECRGTSDD
                     LRSPPGKCGA"
     gene            4473..4841
                     /locus_tag="VF453_00575"
     CDS             4473..4841
                     /locus_tag="VF453_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686160.1"
                     /translation="MSDPTVFRRRNAQALFQAYAESAVAAGASPKGIEQAFAAHVEIS
                     PSMWSQVKSSRPIGDKLARQIERHCGKPAGWLDEERAPAGLSPAEQQFLALALAAWRA
                     TNADGRKALRAQMKALSRGG"
     gene            4947..6560
                     /locus_tag="VF453_00580"
     CDS             4947..6560
                     /locus_tag="VF453_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7686161.1"
                     /translation="MSHADPAASFLEPSPPPSPPADAAPVRIRGGIGRRIGAGFAVVI
                     ALMLGVTAIGLWSGQLVEGRLTRMVAHAWPQAVQAEAMREQLNQTSRSVNRLIFLMTV
                     DDKKREKARILAARAASQAALRSLQASVAEPGGVALLQEVDAALRAYAPQVDAFVALI
                     DDNQTEQARDLALPQLAPLEQRALEALDKLVALQGREIASAGAQAQAAVSDMHAAMLG
                     VSAAAAVLATAIGLFTKRSVTRPLADAVAVARRVADGDLTSTVRAAGDDETGQLLRAL
                     GTMNGNLRHIVAKVRGGTDEMAGTSREIQADCAAILTRTENQARAIEATAASIEELTS
                     TVALNAGNADRANGEMGRTSAIAAEVGQVMGQVVGTMVAIKERSGRIGEIVGVIDGIA
                     FQTNILALNAAVEAARAGEQGRGFAVVAGEVRSLAQRASNASREIRELIQRSVATVED
                     GNHLVDRASAMVQGMVGATGGVASLVSEIAHASQQQHAGIEAFRRSIGELDRMAQQNA
                     EFVGQVSSAADSLHRNMDALVDAVHVFKE"
     gene            6668..7231
                     /locus_tag="VF453_00585"
     CDS             6668..7231
                     /locus_tag="VF453_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686162.1"
                     /translation="MSSLPLPLPTACMPRPSDAIDAYVHAKDRNQPHLMQDAFADDAR
                     LRMLVRTTAISFPPEVRGRAAITDTLVRRFNQVWENVHTLCLGAPPAAGANTFSCDWL
                     VVMSGKRDGGLRAGCGRYDWVFGDDGRVQALDITIDTMESAACELAPVMGWVSALPWP
                     WCARTALRTGAPRVAIVQDVLGRLQAA"
     gene            complement(7393..8439)
                     /locus_tag="VF453_00590"
     CDS             complement(7393..8439)
                     /locus_tag="VF453_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7686163.1"
                     /translation="MNKTLLAIVALTLLDAGAFAQSTVTVYGKVDLGGVLDSGGKAGK
                     SVRLSSGVTGGSRIGFKGVEDLGGGTKASFQLETGYCADSAAGAPNFCTGSNQFMGRQ
                     AHGDLSGAFGQLTAGRVYSLDFINQTTADPFGTGYAGDSQNLFGDKGTTRLNNALQYT
                     TPNFEGFTATAEAALGETTGNWKAGREVGGALTYAKGPLWIDFTILHQDNANGVDASR
                     KNYELGATYDFGVVKIHGLVERAKGEPTGAARPLDALDLLGGVTVPLAGGSLLASYVR
                     HDDRTARSAPGGGDRDASQWALGYNYPLSRRTSVYTAYAHLTDLHGATFTVGNATDAG
                     TGTRGFNLGVVHNF"
     gene            complement(8770..9792)
                     /locus_tag="VF453_00595"
     CDS             complement(8770..9792)
                     /locus_tag="VF453_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7686164.1"
                     /translation="MKKSLIVFAVLSVIGGAASAQSSVTVYGKVDLGGVLDSGSKSGK
                     SVRLSSGVTGGSRIGFKGVEDLGDGYKVAFTLETGYCADSAAGAPNFCTGSNQFMGRQ
                     AHGDLTGAFGAVSAGRQYSLGYTNLTTIDPFGTGYAGQVNNLVDSSGTRVNNSVHYTT
                     PTFSGLTASGEVSFGETTGNWKAGRETGASVNYASGPIYAGLSYYHLDNANGVGAQRQ
                     NVLFGGSYDLGVVKIHGLVQKGSGHPTTGSTVDVLDLMGGVTVPLAGGNILASYVHHN
                     DKTSANKDASQFGLGYNYPLSKRTSVYTAFAKINNQHGATFTVGNATEAGTGNKAFNL
                     GVVHNF"
     gene            complement(10096..13704)
                     /locus_tag="VF453_00600"
     CDS             complement(10096..13704)
                     /locus_tag="VF453_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX7686165.1"
                     /translation="MATRPTEGARWQFWIDRGGTFTDLVGKRPDGTLVTHKLLSENPE
                     QYRDAAVAGIRHLLGLKPGEPITADRVECVKMGTTVATNALLERKGEPTLLVTTRGFR
                     DALRIAYQDRPRLFDRHIVLPELLYERVVEADERVMADGKVLRALDEDALRAQLAEAH
                     AAGLRSVAIVFMHGYRYTAHELAAKRLAQEAGFSQVSVSHEVSPLMKLVGRGDTAVVD
                     AYLSPILRRYVEQVAGEMKGVALYFMQSSGGLTDARAFQGKDAILSGPAGGIVGMVRT
                     AQAAGFERAIGFDMGGTSTDVSHFAGEFERAFETKVAGVRVRAPMMSIHTVAAGGGSV
                     LSFDGARFRAGPESAGANPGPACYRRGGPLAVTDANVMLGKIQPAHFPHVFGPAADEP
                     LDRAIVVRRFEELADRIAEATGKRQSAEAVAEGFVEIAVGSMANAIKKISVARGYDVT
                     RYTLQCFGGAGGQHACLVADALGMTRVFAHPMAGVLSAYGMGLADQALIREAAIELPL
                     ESGIWVAIDRMAALIREAEAQLMQQMAVNGEIAIQRRAHVRYAGTDSALVVPFGDAAT
                     IRAGFEAAYLQRFAFLMEGRELVLEAISVEATAAGDAPPELERSTVPESPAPAADVVR
                     MFSGGQWHDAALVVRAQALPGHVVDGPAIIAEKNATTVVEPGWRARVTALDHLVLERV
                     KPREARKAVGTEVDPVMLEVFNNLFMNIAEQMGLQLQNTAYSVNIKERLDFSCALFDA
                     DGNLIANAPHMPVHLGSMSESIRTVIDRNPGMKPGDVFALNDPYHGGTHLPDVTVVTP
                     VYLGHHRKPTFYVGSRGHQADIGGTTPGSMPPFSTNIEEEGVSIDNFHLVDGGRLREA
                     ETLALLRSGKYPARNPEQNLADLKAQIAANEKGVQELRRMVDQFGEDVVRAYMGHVQD
                     NAEESVRRVITKLKDGEFTLPLDNGARIRVAIRVDAAARSAEIDFTGTSAQLENNFNA
                     PKAVCMAAVLYVFRTLVDDDIPLNAGCLKPLKVIVPEGSMLNPHAPASVVAGNVETST
                     CVTNALYGALGALAAGQCTMNNFTFGNATHQYYETISGGSGAGIGFDGTSVVQTHMTN
                     SRLTDPEVLEFRYPVLLESYTIRAGSGGAGRWKGGDGGIRRVRFLEPMTASILSNGRN
                     VPSFGIAGGKPGALGINRVERADGSVEVLAHIGSTEMGPGDVFVIETPGGGGAGTP"
     gene            complement(13742..14731)
                     /locus_tag="VF453_00605"
     CDS             complement(13742..14731)
                     /locus_tag="VF453_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7686166.1"
                     /translation="MKTAWKVALVLACALVGPPASVAAQVTWKLASGYSEQAFQTVNL
                     RRFADDVATATGGRLVIEVHANNTLAKLGDIPARVEAGDIAAGETILSNLAAEIPVAG
                     ADSVPFIVGSYDDARRLWQAQRPILDEALAKKGLVGLYAVAWPPQGLYSARPVHGAVD
                     LKGAKMRTYNAATQRIAEQLGAHPVDVMMVDLAKAMAAGRIDCMITSGATGIDSHAWE
                     QLKYFYDIHAWYPKNIVLVNQVAFARLDQPTRQAVLQAAAAAEARGWQMSQAAEADSL
                     AQLAAHGMHIDPVDFAFRSELRRFGEHFSVEWVRATGRAANDILIPYYTTASR"
     gene            complement(14806..15591)
                     /locus_tag="VF453_00610"
     CDS             complement(14806..15591)
                     /locus_tag="VF453_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012249144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEX7686167.1"
                     /translation="MTPTPETAPAFALPEPLRLACRTGRFTKPTAGHAPGRIQANMMI
                     VPREHAFDFLLFCQRNPKPCPLVEVLSPGQLEPACAPGASIATDLPGYRVYRDGEFVE
                     ERTDVSELWRDDLVTFLIGCSFSFESQVQAAGVRLRHIDQGRNVAMYRTNVACTPAGI
                     FAGEMVVSMRPIKSRDVAKAVEISARLPIAHGAPVHVGNPRALGVADLMKPDYGDAVE
                     ILDDEVPMFWACGVTPQWVAQRSRLPFCITHAPGKMFVTDLVG"
     gene            complement(15662..16429)
                     /locus_tag="VF453_00615"
     CDS             complement(15662..16429)
                     /locus_tag="VF453_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7686168.1"
                     /translation="MNAQLKEESRVLAAAAPVAGLPLEVENLHVSYDGVPALNGVGLR
                     VEPGRIVALVGGNGNGKSTTLRAIAGLNPLDSGRIRFGDRYVQGLPAYRRVPLGLSLV
                     PEGRRLFPALTVRRNLELGAFTRASKEEVAATLDEMLALFPILAERATQLAGTMSGGE
                     QQMLAIARGLMARPKLLMLDEPSWGIAPKFVTKVLDVIQRVNETGVSILLVEQNLHKA
                     LSIAHRGYVIQTGRIVMEGDAQALMHDEQVRKVYLGL"
     gene            complement(16491..17204)
                     /locus_tag="VF453_00620"
     CDS             complement(16491..17204)
                     /locus_tag="VF453_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7686169.1"
                     /translation="MSLLQLRGVTMRFGGLTANQDVSFDVEAAQIVGLIGPNGAGKTT
                     AFNCVAGYYKPTAGTITLEGRTISGLPPEACARAGVGRTFQVARTFTSMTARENVLAG
                     ALMRDKRVAAAREEADHWLEFVRLQRLAGRPAGSLTISEQRRLAVARALAIHPKLLLM
                     DEVMAGLNPTEVHDMVDVVLAIRGRGIACLIVEHVLEGIMPIADRIVVLERGMKIADG
                     TPREVSSDPAVIAAYLGDE"
     gene            complement(17266..18303)
                     /locus_tag="VF453_00625"
     CDS             complement(17266..18303)
                     /locus_tag="VF453_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7686170.1"
                     /translation="MASKSSRKAWLALAALAVAALVPFAVGNPSTLNLLILVLMSAQL
                     GVAWNILGGYAGQVSLGHAAFYGVGAYTSTLALMNFGISPWFGALGGGLVAAALSLPI
                     GWGCFRLKGHYFAMATIAVAELVQIVFNNWDYGGAAVGLTLPMNHDGWAWLEFSDKRP
                     YYWLALGLLALTLAATWAIERSFLGYYLRAVKDEPDAARSIGIDIGRYKQVALCVSAF
                     FTALGGSLYAQKELYIDPSSVLPTALSIKMALVAILGGIGTLLGPVLGAAVLTTIEEL
                     TRSLFGGTGSGIDTVIYAGLIILIAVFYPSGLVGWFRSRTGRRRARKAAALAREGQLA
                     EVAPETREALQ"
     gene            complement(18308..19174)
                     /locus_tag="VF453_00630"
     CDS             complement(18308..19174)
                     /locus_tag="VF453_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7686171.1"
                     /translation="MTAEILLQTLASGVLIGLVYALVAIGLTMIFGVMDIVNFAHGEF
                     MMLGMYASFWMFAYFALDPLATLPATVLMLFAFGVLLYKLVIRRIIDAPMVSQIFTTF
                     GLMILLRGVAQFLWKPDYRSIDSAWAAGSVHLAGLQLGRPQLIAGVGAVVVTGLVYFF
                     LNRTRLGAALEATAADKEAAQLMGIDSHRMFALAWGIGAACAGAAGALLSTFYSIFPD
                     VGADFILLAFVVVNLGGFGSVTGAFWAGILVGVLEVMGGLLIGPQYKMAVVLVVFLAV
                     LMFRPRGLFGRA"
     gene            complement(19295..20560)
                     /locus_tag="VF453_00635"
     CDS             complement(19295..20560)
                     /locus_tag="VF453_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7686172.1"
                     /translation="MTAFSRVKVTAALAAFWLGTAAHAQTEVKIGVIYPLTGAAASSG
                     AEMKDALELAADIINNGAKGMPDLPFSAGGGLPGLHGAKIKLVFADHQGTPQVGATEA
                     ERLITQEHVVGIIGAYNSNVTATASQVAERYKIPFVNPESSSATLTQRGFKYFFRTTA
                     HDDLFVRNFFEFMKEMQAKKGIKIPSIATFNENTLWGNETTKLEASLAPADGFNLVKQ
                     VTYPAKSTQLTSEVQTLKAAAPAVLLQSSYLGDALLSVRTYKQLGFTPDMILANDAGF
                     ADTEFTRSLGKDADYIISREVWALDLANKNPLIKQVDDLFRSRYKADFNGNSARTFTG
                     LMVMADAINRAGSTDAEAIRKALASTDIPANHLIMPWKGVKFDENGQNVEASGILVQI
                     IDGKYHTVWPTSVASRDVVWPMPAWDKRK"
     gene            complement(20638..21603)
                     /locus_tag="VF453_00640"
     CDS             complement(20638..21603)
                     /locus_tag="VF453_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7686173.1"
                     /translation="MNLRFVEAFYWVATLKSVTRAAEKLSITQSAMSSRIAALESELG
                     VMLLDRSNRQFGLTIPGVRFLVHAQRLLELQREAKAELGTGAGESVPLRIGAIESVLH
                     SWLIPWVEGLRAERPSLALELTVETTPMLTEQMRRGVLDIAFCAMPATGEGVRSRALP
                     TMEMVFVGNAQVHRRARCSLEELAAYDLLTFQRGSHPHVALLDLFRNARLEPKQVHAM
                     SSISAMTQLAEGGFGVATLPRAAAKRLLANRDLRLLTTDPPLPPLPVFASYRPDGAGH
                     SAEAIVRSALAFIAQTEKKSTPVPSTKVQRRTAAVPSKKSMTTKN"
     gene            21977..23851
                     /locus_tag="VF453_00645"
     CDS             21977..23851
                     /locus_tag="VF453_00645"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX7686174.1"
                     /translation="MNRPAMEGLRLNVPAWVKHERLVSWVAEIATLTQAADVHWCDGT
                     QEEYDRLCAQLVAAGTFKKLNPARRANSYLACSDPSDVARVEDRTFICSETKEGAGPT
                     NNWESPEKMRALLQHGQADGTPPLFAGAMRGRTMYVVPFSMGPLGSPISHIGVELSDS
                     PYVAVNMRLMTRMGRGALDVLGADGDFVPCVHTVGAPLEPGQKDVAWPCNKTKYIVHY
                     PESREIWSYGSGYGGNALLGKKCFALRIASNMGRDEGWLAEHMLVLGVTSPEGKKHHI
                     AAAFPSACGKTNFAMLIPPAGMPGWKVTTIGDDIAWIKPSADGRLMAINPEAGYFGVA
                     PGTNTLTNPNCMASLTRDVIFTNVALTDDGDVWWEGMTETPPAHCIDWQGRDWTPAIA
                     KETGAKAAHPNARFTVAATNNPALDPAWDDPKGVAIDAFIFGGRRSTTVPLVTEARDW
                     VDGVYMAATMGSETTAAAVGQQGVVRRDPFAMLPFTGYNMSDYFQHWLNVGERLEAQG
                     ATLPKIFCVNWFRKGADGKFVWPGYGENMRVMEWMLGRIEGRAQGTENVFGTSPRYED
                     INWTGLDFSRAQFDSVIGVDKAAWQQELAMHDELFKTLAYHLPTQLATTKSRIAAALG
                     "
     gene            23944..24789
                     /locus_tag="VF453_00650"
     CDS             23944..24789
                     /locus_tag="VF453_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7686175.1"
                     /translation="MRIQLLSDLHLERAPDWQPPRLPDIDVLVVAGDVGSYQAGSELA
                     GDDFGLARFSPRAPGASWKRTLYLPGNHEFDSLDYDATYARLRGLCAALGIEWLEKET
                     IVAGGVRFVGATLWADFDALASSQASLGAQLKAREKAFRAANFYLGKYTTLKDGAPVL
                     AEGWRAMSLADQDWLRAALGRPFAGPTVAVTHFAPSLRSADPRYGLVPGTAGFCNAMD
                     DFLPLADFWLHGHLHCANDYLASGTRPDGTPWSCRVVANPLGYLKRGEQEDFRPDLVI
                     EIPSR"
     gene            complement(24797..25021)
                     /locus_tag="VF453_00655"
     CDS             complement(24797..25021)
                     /locus_tag="VF453_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686176.1"
                     /translation="MPTASRPAFLRHFGRSLGARREPDLADMGTAFALDEAMDGAGRY
                     ESAVAVEPAAPRPVVHVAPWRMWLGRKLGA"
     gene            25341..25565
                     /locus_tag="VF453_00660"
     CDS             25341..25565
                     /locus_tag="VF453_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686177.1"
                     /translation="MRAVPLQLLTWGTITAAVSSAVLMSSSNQGAVFCSIGYRATSAA
                     IGLDLSDEPPRAGPTVDKPRSTAGKDASAV"
     gene            25744..>27037
                     /locus_tag="VF453_00665"
     CDS             25744..>27037
                     /locus_tag="VF453_00665"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEX7686178.1"
                     /translation="MKVIVLGAGIIGVATAWYLLEEGHEVTVVDRQPDAALETSFANG
                     AQISVCFCEPWANASAPAKVAKWMLQDDSPLLFRPRLDPHQWRWALSFLTQCTDAAFA
                     RNVSQLVALGRYSHESLKTLVERTGISYNRLERGILHFFSSEADYRSGVAAAELMRRQ
                     GVDRRILGRDEVLAVEPALKAFAANIHGGTFTPSDESGDARVFTQQLAARCAARGATF
                     LWGHDVAGFERRDELLEGVVVHERATGARRTLRSDQVVAATGPFTAPLLRQLGIWLNI
                     YPAKGYSATMKLKRPEDASVVSLLDDTRKIAISRLGDHIRIAGTAEMAGYDTSIDGAT
                     SRRRCEALVRRYEQLFPGVADTSEPNFWAGLRPSTPNNLPYIGRSRIANLWLNAGHGT
                     LGWTHGAGSGRAVAELISGRRPQLDYGFCGDALPVRRPR"
BASE COUNT         3884 a         9463 c         9736 g         3954 t
ORIGIN      
        1 ggacccggcc tgggcgggca gatcgcgggc accggcggcc acggcctggg cacgggctcc
       61 ggccgcgccg gcggcacgct caccaagggc ggcggcggca aggcctcgcg ctcgctggag
      121 gaggtgcggc tcgtcctcga acgcaccaag cccgcgctgt acgcgatcta caaccgcgcg
      181 ctgcgcgagg atccgaccct gcagggcaag gtcgtcctcg agctgaccat cgcgcccaac
      241 ggctcggtga ccgaggccca cttcaagtcg agcgagctca agtcgcccga gctggagcag
      301 aagctgctcg cccgcttccg cctgatggag ttcaaggccg aggacgtcga gacgctggtc
      361 acgacctacc cgatcgactt cctgccttcg ggctgaggcc gggcgagggc tcaggcgcgc
      421 gcgtcgaccg gcgccgcgcc ggccatgcgg aacgtgccgc gtcccgccgc cttggccgca
      481 tacagggcct cgtcggcgcg cgccagcagc tcgtccgcct gcgcgcggcc gtcggcctgg
      541 taggcgatgc cgatgctcgc ggtgatcagc agctcccggc cctcgacggc cagcggctgc
      601 gccatggccg cgaggatctt gcgggcgatg gcctgcggct cgaagggcgt gtgcaggccc
      661 tcgaggatca cgacgaactc gtcgccggcg aggcgggcga cggcgtcgac ctcgcgcacg
      721 ctcgcgcgca ggcgctgggc gaagaccgac agcacctggt cgcccgcggc gtggcccagc
      781 gtgtcgttga tcgacttgaa gcggtcgatg tccaggaaga acaggcccac cgcctgctcg
      841 ttgcggcggc tgcgggcaag cgcctcggcg agcttctcgt tgaaggcgta gcggttgggc
      901 aggccggtga gcgcgtcggt gcgcgcctgc agcgcgaggc gcttctccgc cagcttcatc
      961 gccgagacgt cggagacgag cacgtagaag ccggccgtcg tgccgtcggc gccggtgtcg
     1021 ggcaggtaga gcgtgtgcag gtggcggcgg atgcccatca tcgaggtctc gatgtcgaac
     1081 tcgacgtgct cgccggcgag cgcccgccgc accagcgccc ggcgtgcgcc gtgcacctcg
     1141 ggcccgagca cgtccgccat cgtgcggccg acgatctgcg ccggcgcctg gccgatccat
     1201 tcccgatacg tgccgttggc gaactggtag cgctcgtcgc ggtcgacgta ggagatcagc
     1261 accggcaggt tgtcggcgat ggtgcgcagg cgctgctcgc tgcgcgccag ggccagctcg
     1321 acgtcgcggc gcgcggtcag gtccagcacc atgccgtaga tgccctggac gcgcccgtcc
     1381 tcgtcgaggt cgggaacgta gtggatcagc gcgtgcatgc gccgccccag gatgacgacc
     1441 tcgcgctcgg acgagctggc ctcgcccgcg agcgcccgcc gcatcgcgcc gtccaggccc
     1501 gcgaaggcct cggcgccgac gacatcggcc accccgcggc ccaccatggc ggcgtggtcg
     1561 acatcgaacc agcgcccgta ggtcgcgttg gtgaacgtgt atcgcagccc ggtgtcgatg
     1621 tgcgagacga gggccggcag gttgtccgtg acggtgcgca gccggcgctc gctctgcgcg
     1681 agccggcgct cggcgcgctt gcgctcgctg atgtcgtgca tgaacgcgta cgcgaccagg
     1741 ccgccgtccg tgcgcagcgc cgcggcggac agctgcaccg ggatctcggc gcccgacttg
     1801 tgcagcacgg cgcgttcgac gcgatggccc gccgacgcgc cgcggccgtg cgtgaacgcg
     1861 tcgacgcccg tgcgccactc gtcgcgcagg ccgggcggcg tgagcaggtc gtcgaccatc
     1921 tgcccgatcg cctcggcgcg cgtccagccg aaggtctgct ccgcgcgccg gttccattcg
     1981 ctgatgcggc cggcctcgtc gatcgcgacg taggcgtcgg gcgcgtgcgt gaggatcgcg
     2041 cgcaggcgct cctcgctttg ccagcgctcg cgcacggcct gcgacagctc gccgttcagg
     2101 cgctcgagcg ccgcggtgcg ctcggcgacg cgggcctcga ggccggcgtt gaggtcgcgc
     2161 aggtgcgcgg cgtcctcgcg ctggcggcgg gcgtagcgat ccagcgcgtg gccgagcagc
     2221 tcggcctcgc gatagagcgg cagtcctcgc gcgggcggcg ccgcatcgac cacgtcgagc
     2281 tcggcttcga gcgcgcgcag cggcgtcgcc aagcgccgcg cgagcagcca cgccaacggg
     2341 gcgagcacga ggcacagcag cgccgccgcg aggcgcgtgc gcagctgcag cgcatggaag
     2401 gtcgccatcg ccaccggttc cggctgatgc aacacgacac gccagccgag gcccggatag
     2461 cgacccgagc cctgcgtgcg ggactcgacg gcgaactcgc gcgccgcgtc gaacgcgggc
     2521 agcttgcggc cctggagcgc gggcggcccg agcagcacga cgccgtcggc gccgacgacc
     2581 agcgcctcgg cctggtgctg cgccagctcg cggtcgacga ggtcacgctc gatctgcgcc
     2641 gcccactgcc agcttaggtg cccggcgagg atgccgcgca gcgagccgtc ggcccgatag
     2701 acgggcgtgg cgatgtcgac gaagcgccaa ggttccgcct gccggggcag aagcccggcc
     2761 agcagggccg cggcgtggac gtcgccgacg tacatgccgt gccgcgctcc cgcgaaccac
     2821 ggacgcgccg agacgtcgac gccctgcagc agcccgtcga ccgacgcgat caccttgcca
     2881 tcggtcccgg ccagtcccat ccacgcgaac tgcgggaagc tgccgcgcat cgcctccagc
     2941 gcgcgccgca ccgcggccgg gtcgttgccg tcggcgacct ggtcgaggcg gcccagcacg
     3001 cgcacgtcct cgaagttctg cgccatgccg cgatcgagcg cgtcgcgcag ctcgtccgcg
     3061 tcggattgca ggaactgggt ggcctggcgc cgctcctcct ggcgcacgaa ggattcgatc
     3121 acgagcgtgc aggtcagcac ggcgaccatg gtggcgccga gcacgctgac gaacagccac
     3181 gtggcgagcg tcggccgcgg cgggcggctg tccgcgggcg gcggggaggc gggacggcgg
     3241 ttcacgcccc gatatcggcc ggtcgagcgc cccctgaagc gggaaaagac gggcgtcgcg
     3301 cggccatttg cgcgcgacgc ccgcccgccg gcttgcgttc agcgcagccg cgagcgcttc
     3361 ggcaggcggg cggccagcca cccgtgcacg tccgcccgca tcgaacggcc ggccagcagg
     3421 aagtcctcga accggcccgg cacgtacgga acgccctcct ttccgtggcc gatgccgcgc
     3481 atgtcagcgg tggtagtcct ttctttcgcg ggcgcctaaa gcacgcttct tgcaggcagc
     3541 actgcccgaa gcgccttcgc gagccgcaga agcagggatc ctggcgtccc agcttctcga
     3601 gcagctcctt gtcgccgtgc acgacgcgcg tgccgcgctt gacccccgtc tcgctcggga
     3661 acccgcgccg gcgcttgctc atgggctcgg gcgatgtcgt gctcgtgaaa ccagtagtcc
     3721 tcgtggcgga tgcgcatgat gtccccctga tgtctcggtt aggaagtgaa ggttccggtg
     3781 acgggttccg gagccgccca atcgttgcgg ccggttttcg ccgcgacggc gtgagcgctc
     3841 gccgcggctt cgggtcgatc tgccggacgc cttcgcgacg cccactccgg ctgtgcccac
     3901 cgcgcgagcc ctcccgtcgg aggcggtgtc tcgctgcacg ttggccaggt tgccccgccg
     3961 cgcacatccc gggccgcgca cacctcggcg tctcgcggca ccggagtgcg gaggcacacc
     4021 cggagtgggc ggacgcgcgc gtccgcggct gaacaggttc gccctgcggc ggcgcgaggc
     4081 cgaccggttc gggcgacgaa atggaaagag gcccggatcc ttcgcggagt ccgggcctct
     4141 gaggtgtcag agggcgaagg gcgcgccggt caggcgccgc actttcccgg aggactgcga
     4201 aggtcgtcgc tggtgccgcg gcattcgccg ccggcgtcgg cgcccgcttt cagggacatc
     4261 gactgcaggg ccggcgcgaa cgcacgcgtc ccggcgacgg ggcggtggta gcggagcgat
     4321 tgcaggcggt tcacgatatg tccttgaagc gtcggtcggc cgggctctct cgagcgatgg
     4381 agcgaatggt aaatagtctt tactcatcgc gcaagccccg cgacaatgcg gccgcttccg
     4441 tcgttgcgtt ccgatcacac ccgtcccccg ccgtgagcga tcccaccgtc ttccgccgcc
     4501 gcaacgcgca ggcgttgttc caggcctacg ccgaatccgc ggtcgccgcc ggcgcctcgc
     4561 ccaagggcat cgagcaggcc ttcgccgcgc atgtcgagat ctcgcccagc atgtggagcc
     4621 aggtcaagtc ctcgcgcccg atcggcgaca agctcgcgcg ccagatcgag cgccactgcg
     4681 gcaagcccgc cggctggctc gacgaggagc gcgcgcccgc cggcctgtcg ccggccgagc
     4741 agcagttcct cgcgctcgcc ctggccgcct ggcgcgccac caatgccgac ggccgcaagg
     4801 cgctgcgggc gcagatgaag gcactctcgc ggggcggatg agggccgcgg agcacgcggg
     4861 aaatcgttgt gccctcatcg aaatttccgc ttcaaccggg ccgccggcgc ggtcgatacc
     4921 cgggtcaggc catccaggag tccgccatgt cccacgccga tcccgccgca tccttcctcg
     4981 aaccgagccc gccgcccagc ccgccggcgg acgccgcacc cgtgcgcatc cgaggaggca
     5041 tcggccggcg catcggcgcg ggcttcgccg tcgtcatcgc gctgatgctg ggcgtcacgg
     5101 cgatcgggct gtggtcgggc cagctggtgg aaggccggct cacgcgcatg gtcgcccacg
     5161 cgtggccgca ggccgtccag gccgaggcga tgcgcgagca gctgaaccag acctcgcgca
     5221 gcgtcaaccg cctgatcttc ctgatgacgg tggacgacaa gaagcgcgag aaggcccgca
     5281 tcctcgcggc gcgcgcggcg tcgcaggcgg cgctgaggtc gctgcaggcc tcggtggccg
     5341 agccgggcgg cgtcgcgctg ctgcaggagg tcgacgccgc gctgcgcgcc tacgcgccgc
     5401 aggtggatgc cttcgtcgcc ctgatcgacg acaaccagac cgagcaggcg cgcgacctcg
     5461 cgctgccgca gctcgcaccg ctcgagcagc gcgcgctcga ggcgctcgac aagctcgtcg
     5521 cgctgcaggg ccgcgagatc gcctccgcgg gcgcgcaggc gcaagccgcg gtctccgaca
     5581 tgcacgccgc gatgctgggc gtcagcgccg cggccgccgt gctggccacg gcgatcggcc
     5641 tgttcaccaa gcgcagcgtg acgcggcccc tcgccgatgc cgtggcggtc gcgcggcggg
     5701 tggccgacgg cgatctcacc tcgaccgtgc gggctgccgg cgacgacgag accggccagc
     5761 tgctgcgcgc gctcggcacc atgaacggca acctgcgcca catcgttgcc aaggtgcgcg
     5821 gcggcaccga cgagatggcc ggcacctcgc gcgagatcca ggccgactgc gctgcgatcc
     5881 tcacgcgcac cgagaaccag gcgcgcgcga tcgaggcgac cgccgcgtcg atcgaggagc
     5941 tcacgtccac cgtcgccctc aacgccggca atgcggaccg cgccaacggc gagatggggc
     6001 gcacgtcggc catcgcggcg gaggtcgggc aggtgatggg gcaggtcgtg ggcacgatgg
     6061 tggcgatcaa ggagcgctcg ggtcgcatcg gagaaatcgt cggcgtcatc gacgggatcg
     6121 cgttccagac caacatcctg gcgctcaacg cggcagtcga ggccgcgcgc gccggcgagc
     6181 aggggcgcgg cttcgccgtc gtcgccggcg aggtgcgctc gctcgcgcag cgggcgtcca
     6241 atgcgtcgcg cgagatccgc gagctgatcc agcgctcggt cgcgacggtc gaggacggca
     6301 accacctcgt cgaccgcgcc tcggcgatgg tgcagggcat ggtcggcgcg accgggggcg
     6361 tggcgtcgct cgtctccgag atcgcgcacg cgagccagca acagcacgcg ggcatcgagg
     6421 cgttccggcg ctcgatcggc gaactcgacc ggatggcgca gcagaacgcc gagttcgtcg
     6481 gccaggtctc gagcgccgcc gacagcctgc accgcaacat ggacgccctc gtcgacgccg
     6541 tccacgtgtt caaggaatga catcatcgat tttctcgatg ccgaaccagc gagattctgc
     6601 gcttgggctt cgcgggcgcg cgatcctaga attttcgaca tccaccgcca gcaaggggag
     6661 aacgcgcatg tcgtccctgc ctctgcccct cccgaccgcc tgcatgccgc gcccctcgga
     6721 cgccatcgac gcctacgtcc atgcgaagga ccgcaaccag ccgcacctga tgcaggacgc
     6781 cttcgcggac gacgcgcgcc tgcgcatgct cgtgcgcacg acggcgatct cgttcccgcc
     6841 cgaggtgcgc ggccgcgcgg cgatcaccga cacgctggtg cgccgcttca accaggtctg
     6901 ggagaacgtg cacacgctgt gcctcggcgc gccgcccgcg gcgggtgcga acacgttctc
     6961 gtgcgactgg ctggtggtga tgtcgggcaa gcgcgacggc ggcctgcgtg ccggctgcgg
     7021 acgctacgac tgggtcttcg gcgacgacgg ccgcgtgcag gcgctggaca tcaccatcga
     7081 caccatggag tccgccgcct gcgagctcgc gcccgtgatg ggctgggtct cggcgctgcc
     7141 gtggccgtgg tgcgcgcgca ccgccttgcg caccggcgcg ccgcgggtcg cgatcgtgca
     7201 ggacgtgctc ggccggctgc aggccgcctg aggcgcgcgc cgggcgcggt cgccgcggtc
     7261 ggcctgcccg gccatccagc tcgtccctcc gccgtcgcca ggaggaaaaa agcccgtccg
     7321 cgctcaagtc ggacgggcaa cccccggttc gccgcggggg cgtcgcgccc gcggcgtctt
     7381 cggatgcgac gttcagaagt tgtggacgac acccaggttg aagccgcggg tgccggtgcc
     7441 ggcgtcggtc gcgttgccca ccgtgaaggt cgcgccgtgc aggtcggtca ggtgcgcgta
     7501 cgcggtgtag accgaggtgc gccgggacag cgggtagttg tagcccagcg cccactgcga
     7561 ggcgtcgcgg tcgccgccgc cgggcgcgct gcgcgcggtg cggtcgtcat ggcggacata
     7621 gctggccagc aggctgccgc cggccagcgg gacggtcacg ccgcccagca ggtccagcgc
     7681 gtcgagcggg cgcgcggcgc cggtcggctc gcccttggcg cgctcgacca ggccgtggat
     7741 cttcacgacg ccgaagtcgt aggtcgcgcc gagctcgtag ttcttgcgcg acgcgtccac
     7801 gccgttggca ttgtcctggt gcaggatcgt gaagtcgatc cacagcgggc ccttggcgta
     7861 cgtcagcgcg ccaccgacct cgcgaccggc cttccagttg cccgtcgtct cgccgagggc
     7921 ggcctccgcg gtggccgtga agccttcgaa gttcggcgtc gtgtactgga gggcgttgtt
     7981 caggcgcgtc gtgcccttgt cgccgaagag gttctgcgaa tcgccggcat agcccgtgcc
     8041 gaaggggtcg gcggtcgtct ggttgatgaa gtcgaggctg tagacgcggc ccgccgtgag
     8101 ctggccgaac gcgccgctca ggtcgccgtg ggcctggcgg cccatgaact ggttggagcc
     8161 ggtgcagaag ttgggcgcgc cggcggcgct gtcggcgcag tagccggtct cgagctggaa
     8221 cgacgccttc gtgccgccgc ccaggtcctc gacgcccttg aagccgatgc gcgagccgcc
     8281 cgtgacgccc gaggacaggc gcaccgactt gccggccttg ccgccgctgt cgaggacgcc
     8341 gccgaggtcg accttgccgt agacggtgac cgtggattgc gcgaaggcgc cggcgtcgag
     8401 cagcgtcagc gcgacgatgg cgaggagagt cttgttcatg attcgatctc gggtgagtgc
     8461 gtggatggga tggatcgatc cggcaaaaat tgtcgagttg gatccgcgtg ccgggcaacg
     8521 cgaaatttct cgacgccaca atcgattttt tcaatccacg catcgccggg ttcgaggtcg
     8581 gaatcccgtc gatggcgcgc catcggaatt tccgtggccg atgcgcggcg ccccggaaat
     8641 gacgaagggc gccggcattg gctgccggcg ccctggaaaa aaacccgtcc gggctcaagt
     8701 cggacgggca agacctcgtg cgtcgcggcg ccaggagtcg gacgcgcgac gcaggggcga
     8761 attcgcgctt cagaagttgt ggacgacgcc caggttgaac gccttgttgc cggtgccggc
     8821 ctcggtggcg ttgccgacgg tgaaggtggc accgtgctgg ttgttgatct tggcgaaggc
     8881 cgtgtagacc gacgtgcgct tggacagcgg gtagttgtag cccaggccga actgcgacgc
     8941 atccttgttg gcgctggtct tgtcgttgtg gtgcacgtac gacgcgagga tgttgccgcc
     9001 ggccagcggc acggtcacgc cgcccatcag gtcgaggacg tcgacggtcg agccggtcgt
     9061 cggatggccg ctgcccttct gcacgaggcc gtggatcttc acgacgccga ggtcgtagct
     9121 gccgccgaac aggacgttct ggcgctgcgc gccgacgccg ttggcgttgt cgaggtggta
     9181 gtacgacagg ccggcgtaga tcgggcccga ggcgtagttg acggacgcgc cggtctcgcg
     9241 gccggccttc cagttgccgg tcgtctcgcc gaacgagact tcgcccgagg cggtcaggcc
     9301 gctgaaggtc ggcgtcgtgt agtgcacgga gttgttcacg cgcgtgccgg acgagtcgac
     9361 caggttgttg acctggcccg catagccggt gccgaacggg tcgatcgtgg tgaggttcgt
     9421 gtagccgagg ctgtactggc ggccggcgct gacggcaccg aaggcgcccg tcaggtcgcc
     9481 gtgggcctgg cggcccatga actggttgga gcccgtgcag aagttcgggg cgccggcggc
     9541 gctgtcggcg cagtagccgg tctcgagcgt gaacgcgacc ttgtagccgt cgccgagatc
     9601 ctcgacgccc ttgaagccga tgcgcgagcc gccggtgacg ccggaggaca ggcgcaccga
     9661 cttgccggac ttgctgccgc tgtcgaggac gccgccgagg tcgaccttgc cgtagacggt
     9721 gaccgacgat tgcgccgacg cggcgccgcc gatcacggac aggacggcga aaacgatgag
     9781 agatttcttc atggtgggat ctcgttggat gttgcggggt gacgcgcggc gcgcccctcg
     9841 gtgcgcttgg cgctcacggg ccgggacgtc gctggggact cgcgtcgccg tgccgctcgt
     9901 gagatgtcgg ggcggattgt cgaggtcggt ctcgcgaccc cgcaaatgcg aaattttttg
     9961 acccaacagc gtgttcgcct tggcagggca tttcacgcag cggtcacgca catcggcgaa
    10021 aagtggcacg catgaacgaa aaagggcgcc ggcatcgctg ccggcgcccc tcgcccggac
    10081 gatcccgggc cgggttcagg gcgtgcccgc gccgccgccg ccgggggtct cgatgacgaa
    10141 gacgtcgccc ggccccatct cggtggagcc gatgtgcgcg agcacctcga cgctgccgtc
    10201 cgcgcgctcg acgcggttga tgccgagcgc gcccggcttg ccgccggcga tgccgaacga
    10261 aggcacgttg cggccgttgc tcaggatgga ggccgtcatc ggctcgagga atcgcacgcg
    10321 ccggatgccg ccgtcgccgc ccttccagcg acccgcgccg ccggagccgg cgcggatcgt
    10381 gtagctctcc agcaacacgg ggtagcggaa ctccagcacc tcgggatcgg tcagccgcga
    10441 gttcgtcatg tgcgtctgca cgacgctcgt gccgtcgaag ccgatgcccg cgccgctgcc
    10501 gcccgagatc gtctcgtagt actggtgcgt cgcgttgccg aaggtgaagt tgttcatcgt
    10561 gcactggccg gccgcgagtg ccccgagcgc gccgtacagc gcgttggtga cgcaggtcga
    10621 ggtctcgacg ttgcccgcga ccaccgacgc cggggcgtgc gggttgagca tcgagccttc
    10681 cggcacgatc accttcagcg gcttcaggca gccggcgttc agcgggatgt cgtcgtccac
    10741 cagcgtgcgg aacacgtaga gcaccgcggc catgcacacg gccttgggcg cgttgaagtt
    10801 gttctccagc tgcgccgacg tgcccgtgaa gtcgatctcc gccgatcgcg ccgcggcgtc
    10861 gacgcggatc gccacgcgga tgcgcgcgcc gttgtccagc ggcagcgtga actcgccgtc
    10921 cttcagcttc gtgatgacgc ggcgcaccga ctcctcggcg ttgtcctgca cgtggcccat
    10981 gtaggcgcgc acgacgtcct cgccgaactg gtcgaccatg cggcgcaact cctgcacgcc
    11041 cttctcgttg gcggcgatct gggccttcag gtcggcgagg ttctgctccg ggttgcgcgc
    11101 tgggtacttg ccgctgcgca gcagggcgag cgtctcggcc tcgcgcaggc ggccgccgtc
    11161 gacgaggtgg aagttgtcga tcgagacgcc ctcctcctcg atgttcgtcg agaacggcgg
    11221 catcgagccc ggcgtggtgc cgccgatgtc ggcctggtgt ccgcgcgagc cgacgtagaa
    11281 cgtgggcttg cggtgatgcc cgaggtagac cggcgtgacc accgtcacgt cgggcaggtg
    11341 cgtgccgccg tggtacgggt cgttgagggc gaagacatcg cccggcttca tgcccgggtt
    11401 gcggtcgatc accgtgcgga tcgactcgct catcgagcca aggtgcaccg gcatgtgcgg
    11461 cgcgttggcg atgaggttgc cgtcggcgtc gaacagcgcg cacgagaagt ccagccgctc
    11521 cttgatgttg accgagtagg cggtgttctg cagctgcagg cccatctgct cggcgatgtt
    11581 catgaacagg ttgttgaaca cctcgagcat caccgggtcg acctcggtgc ccacggcctt
    11641 gcgcgcctcg cgcggcttca cgcgctcgag caccaggtgg tcgagcgccg tgacgcgcgc
    11701 acgccagccg ggctcgacga cggtcgtcgc gttcttctcg gcgatgatcg cgggcccgtc
    11761 gacgacgtgg cccggcagcg cctgcgcccg cacgacgagc gccgcgtcgt gccattgccc
    11821 gccgctgaac atgcgcacga cgtcggccgc cggcgcgggc gactcgggca cggtggagcg
    11881 ctccagctcg ggcggcgcgt cgccggcggc ggtggcctcc accgagatcg cctccagcac
    11941 cagctcgcgg ccctccataa gaaacgcgaa gcgctgcagg taggcggcct cgaagccggc
    12001 gcggatcgtc gccgcgtcgc cgaagggcac gacgagggcg gagtccgtgc ccgcgtagcg
    12061 cacgtgggcg cggcgctgga tcgcgatctc gccgttgacg gccatctgct gcatcagctg
    12121 cgcctcggcc tcgcggatca acgccgccat gcggtcgatc gcgacccaga tgcccgactc
    12181 gagcggcagc tcgatcgcgg cctcgcggat cagcgcctgg tcggccaggc ccatgccgta
    12241 ggccgagagc acgcccgcca tcggatgcgc gaacacgcgc gtcatgccca gcgcgtcggc
    12301 gacgaggcag gcgtgctggc cgcccgcgcc gccgaagcac tgcagcgtgt agcgcgtgac
    12361 gtcgtagccg cgcgccaccg agatcttctt gatcgcgttg gccatcgagc cgaccgcgat
    12421 ctcgacgaag ccctcggcga cggcctcggc cgactggcgc ttgcccgtcg cctcggcgat
    12481 gcggtcggcg agctcctcga agcggcgcac gacgatcgcg cggtcgagcg gctcgtcggc
    12541 cgccggcccg aagacgtgcg ggaagtgcgc cggctggatc ttgccgagca tcacgttcgc
    12601 gtccgtcacg gcgagcgggc cgccgcgacg gtagcaggcc gggcccgggt tcgcgcccgc
    12661 gctctcgggg ccggcccgga agcgcgcgcc gtcgaagctc agcaccgagc cgccgcccgc
    12721 ggcgacggtg tggatgctca tcatcggcgc gcgcacgcgc acgccggcga ccttggtctc
    12781 gaacgcgcgc tcgaactcgc ccgcgaagtg gctcacgtcg gtcgaggtgc cgcccatgtc
    12841 gaagccgatg gcgcgctcga agccggcggc ctgcgccgtg cgcaccatgc cgacgatgcc
    12901 gccggccggg cccgacagga tcgcgtcctt gccctggaag gcgcgagcgt ccgtcaggcc
    12961 gcccgaggac tgcatgaagt acagcgcgac gcccttcatc tcgccggcca cctgctccac
    13021 gtagcggcgc aggatcggcg acaggtaggc gtcgacgacg gccgtgtcgc cgcggccgac
    13081 cagcttcatc agcgggctca cctcgtgcga gacgctcacc tgcgagaagc cggcctcctg
    13141 cgcgaggcgc ttggccgcga gctcgtgggc ggtgtagcgg tagccgtgca tgaagacgat
    13201 ggcgacgctg cgcaggccgg cggcgtgtgc ctcggcgagc tgcgcgcgca gcgcgtcctc
    13261 gtcgagcgcg cgcagcacct tgccgtcggc catcacgcgc tcgtcggcct cgaccacgcg
    13321 ctcgtacagc agctcgggca gcacgatgtg gcggtcgaac aggcgcgggc ggtcctggta
    13381 ggcgatgcgc agtgcgtcgc ggaagccgcg cgtcgtcacg agcagcgtcg gctcgccctt
    13441 gcgctcgagc agcgcgttgg tcgcgacggt cgtgcccatc ttcacgcact cgacgcggtc
    13501 ggcggtgatc ggctcgcccg gcttcaggcc gagcaggtgg cggatgccgg cgaccgccgc
    13561 gtcgcgatac tgctcggggt tctccgacag cagcttgtgc gtgacgagcg tgccgtcggg
    13621 ccgcttgccg acgaggtcgg tgaacgtgcc gccgcggtcg atccagaact gccagcgggc
    13681 gccttcggtg ggtcgtgttg ccatggtgtg ctcgtgcgtg gggatgcggg gggacggcgc
    13741 gtcagcgcga cgcggtggtg tagtacggga tgaggatgtc gttggcggcg cgcccggtgg
    13801 cgcgcaccca ttcgacggag aagtgttcgc cgaaacggcg cagctcgctg cggaacgcga
    13861 aatcgaccgg gtcgatgtgc atgccatgcg cggccagctg cgcgagcgag tcggcctcgg
    13921 cagcctggct catctgccag ccacgcgcct cggcggccgc cgcggcctgc agcacggcct
    13981 ggcgggtggg ctggtcgagg cgcgcgaacg cgacctggtt gaccagcacg atgttcttcg
    14041 ggtaccaggc atggatgtcg tagaagtact tcagctgctc ccacgcgtgg ctgtcgatgc
    14101 cggtcgcgcc ggaggtgatc atgcagtcga tgcggcccgc ggccatcgcc ttggccaggt
    14161 ccaccatcat gacgtcgacc ggatgcgcgc ccagctgctc ggcgatgcgc tgcgtcgccg
    14221 cgttgtaggt gcgcatcttc gcgcccttga ggtcgaccgc gccgtggacg ggccgggccg
    14281 agtagagccc ctgcggcggc cacgccaccg cgtacagccc gaccaggccc ttcttcgcga
    14341 gcgcctcgtc gaggatcggc cgctgcgcct gccacagccg gcgcgcgtcg tcgtagctgc
    14401 cgacgatgaa gggcaccgag tccgcgcccg cgaccgggat ctcggcggcg aggttgctca
    14461 ggatcgtctc gccggcggcg atgtcgcccg cctcgacgcg cgccgggatg tcgcccagct
    14521 tcgcgagcgt gttgttcgca tgcacctcga tgaccagcct gccgcccgtg gccgtggcca
    14581 cgtcgtcggc gaagcgccgc aggttgaccg tctggaaggc ctgctccgag tagccgctcg
    14641 ccagcttcca ggtgacctgg gccgcgacgg atgccggcgg gccgacgagg gcgcaggcga
    14701 ggacgagggc gactttccag gcggtcttca tggcggactc cttcgacggc gacaggctcg
    14761 atggaacgcg gacgggcggg agcggggtgc ggtatgcgcg gtgcgtcagc cgacgaggtc
    14821 ggtgacgaac atcttgcccg gcgcgtgcgt gatgcagaac ggcagccggc tgcgctgcgc
    14881 gacccactgc ggggtgacgc cgcaggccca gaacatcggc acctcgtcgt ccaggatctc
    14941 cacggcgtcg ccgtagtccg gcttcatcag gtccgcgacg cccagcgcgc gcgggttgcc
    15001 gacgtgcacc ggcgcgccat gcgcgatcgg cagccgcgcc gagatctcga cagccttcgc
    15061 gacgtcgcgc gacttgatcg gccgcatgct caccaccatc tcgccggcga agatgccggc
    15121 cggcgtgcag gcgacgttcg tgcggtacat cgcgacgttg cgcccctggt cgatgtggcg
    15181 cagccgcaca ccggccgcct gcacctgcga ctcgaacgag aagctgcagc cgatcaggaa
    15241 ggtgacgagg tcgtcgcgcc agagctcgga gacgtcggtg cgctcctcga cgaattcgcc
    15301 gtcgcggtag acgcggtagc cgggcaggtc ggtcgcgatg ctcgcgccgg gcgcgcaggc
    15361 gggctcgagc tggccggggg acagcacctc gaccagcggg cacggcttcg gattgcgctg
    15421 gcagaacagc aggaagtcga aggcgtgctc gcgcggcacg atcatcatgt tggcctggat
    15481 gcgccccggc gcgtggccgg cggtcggctt cgtgaagcgg ccggtgcggc aagcgagccg
    15541 cagcggctcg ggcagcgcga aggcgggcgc ggtctccggg gtcggtgtca tggtgtcttt
    15601 cctaacttct aatttcagcg gaaacaggtg tcgacgcgcg tgggcggcga gcgcgcgggg
    15661 ctcagagccc gaggtagacc ttgcgaacct gctcgtcgtg catcagcgcc tgggcgtcgc
    15721 cctccatcac gatgcggccg gtctggatga cgtagccgcg gtgcgcgatg gacagcgcct
    15781 tgtgcaggtt ctgctcgacc agcaggatcg aaacgccggt ctcgttgacg cgctggatca
    15841 cgtcgagcac cttggtgacg aacttcggcg cgatgcccca cgagggctcg tcgagcatca
    15901 gcagcttcgg ccgcgccatc aggccgcggg cgatcgcgag catctgctgc tcgccgccgc
    15961 tcatcgtgcc ggcgagctgg gtcgcgcgct cggcgaggat gggaaagagg gcaagcatct
    16021 cgtccagcgt cgcggcgacc tcctccttgc tcgcgcgcgt gaaggcgccg agctcgaggt
    16081 tgcgccgcac cgtcagcgcg gggaacaggc gccggccctc gggcaccagc gacagcccga
    16141 gcggcacgcg ccggtacgcg ggcaggccct gcacgtagcg gtcgccgaag cggatgcggc
    16201 ccgagtcgag cgggttgagc cccgcgatcg cgcgcagcgt cgtcgacttg ccgttgccgt
    16261 tgccgccgac cagcgcgacg atgcgccccg gctcgacgcg caggccgacg ccgttgagcg
    16321 ccggcacgcc gtcgtagctc acgtgcaggt tctcgacctc cagcggcagg ccggcgaccg
    16381 gcgccgcggc ggcgagcacc cggctttctt ccttcagctg ggcattcatt tggaacctcc
    16441 gtccttcggg ctgcggtgcg agcgcctcgg gagcggccgt gcggcgctca tcactcgtct
    16501 cccaggtagg ccgcgatcac ggcggggtcg ctggagacct cccgcggcgt gccgtcggcg
    16561 atcttcatgc cgcgctcgag cacgacgatc cggtcggcga tgggcatgat gccctcgagc
    16621 acgtgctcga cgatcaggca cgcgatgccg cggccgcgga tcgccagcac gacgtcgacc
    16681 atgtcgtgca cctcggtcgg gttcaggccc gccatcacct cgtccatcag cagcagcttc
    16741 ggatggatgg ccagggcgcg cgcgacggcg aggcggcgct gctcgctgat cgtcaggctg
    16801 ccggcgggcc ggccggccag gcgctgcagg cgcacgaact cgagccagtg gtcggcctcc
    16861 tcgcgcgccg cggcaacgcg cttgtcgcgc atcagcgctc cggcgagcac gttctcgcgc
    16921 gccgtcatgc tcgtgaacgt gcgcgcgacc tggaaggtgc ggccgacgcc ggcgcgggcg
    16981 cacgcctcgg gcggcaggcc ggagatcgtg cggccttcca gcgtgatcgt tcccgcggtc
    17041 ggcttgtagt agcccgcgac gcagttgaac gcggtcgtct tgccggcgcc gttcgggccg
    17101 atcaggccga cgatctgcgc ggcctcgacg tcgaaggaca cgtcctggtt ggcggtcagc
    17161 ccgccgaagc gcatcgtgac gcctctcagt tggagcaggc tcatcgggaa acctccgtcc
    17221 ttcggactgc ggtgcgagct cccgccggag cggccgggcg cgggttcatt ggagcgcctc
    17281 ccgggtttcg ggcgcgacct cggcgagctg gccctcgcgc gccagcgcgg ccgccttgcg
    17341 cgcgcggcgc cggcccgttc ggctgcgaaa ccagccgacg aggccgctcg gatagaagac
    17401 ggcgatcagg atgatcagcc ccgcgtagat cacggtgtcg atgccgctgc cggtgccgcc
    17461 gaagagcgag cgcgtgagct cctcgatggt cgtcagcacc gcggcgccca gcaccggacc
    17521 gagcagcgtg ccgatgccgc ccaggatcgc caccagcgcc atcttgatcg acagggccgt
    17581 cggcagcacc gagctcgggt cgatgtagag ctccttctgc gcatacaggc tgccgcccag
    17641 cgcggtgaag aaggcgctca cgcacagcgc gacctgcttg tagcggccga tgtcgatgcc
    17701 gatcgagcgc gcggcgtccg gctcgtcctt gacggcgcgc aggtagtagc cgaggaagct
    17761 gcgctcgatg gcccaggtcg ccgccagcgt gagcgcgagc aggccgagcg cgagccagta
    17821 gtacgggcgc ttgtcggaga actcgagcca cgcccagccg tcgtggttca tcggcagcgt
    17881 gaggccgacg gccgcgccgc cgtagtccca gttgttgaac acgatctgca ccagctccgc
    17941 gaccgcgatc gtggccatcg cgaagtagtg gcccttcagg cggaagcagc cccagccgat
    18001 cggcaggctc agggccgccg cgaccaggcc gccgccgagc gcgccgaacc acgggctgat
    18061 gccgaagttc atcagcgcga gcgtcgacgt gtaggcgccg acgccgtaga acgccgcgtg
    18121 gccgagcgac acctgcccgg cgtagccgcc gaggatgttc cacgccacgc ccagctgcgc
    18181 ggacatcagc acgaggatca gcaggttcag cgtcgacgga ttgccgaccg cgaacggcac
    18241 gagggccgcg acggccagcg ccgccagcgc gagccaggcc ttcctggatg atttggatgc
    18301 catggcttca ggccctcccg aacaggccgc gcggccggaa catcagcacg gcgaggaaca
    18361 cgaccagcac caccgccatc ttgtactgcg gcccgatcag caggccgccc atcacctcga
    18421 gcacgcccac caggatgccg gcccagaacg cgccggtcac gctgccgaag ccgcccaggt
    18481 tcacgacgac gaaggcgagc aggatgaagt cggcgccgac gtcggggaag atcgagtaga
    18541 acgtcgacag cagcgcaccg gccgcgccgg cgcaggccgc gccgatgccc caggcgagcg
    18601 cgaacatgcg gtgcgagtcg atgcccatca gctgcgcggc ctccttgtcg gcggcggtgg
    18661 cctccagcgc ggcgccgagc cgcgtgcggt tgaggaagaa gtagaccagc cccgtcacca
    18721 cgaccgcgcc gacgcccgcg atcagctgcg gccgcccgag ctgcaggccc gcgaggtgca
    18781 ccgagccggc ggcccaggcg ctgtcgatgg agcggtagtc cggcttccag aggaactgcg
    18841 ccacgccccg cagcaggatc atcaacccga aggtcgtgaa gatctggctg accatgggcg
    18901 cgtcgatgat gcgcctgatc acgagcttgt agagcagcac gccgaacgcg aacagcatca
    18961 gcacggtggc cggcagcgtc gcgagcgggt cgagcgcgaa gtacgcgaac atccagaagc
    19021 tcgcgtacat cccgagcatc atgaactcgc cgtgggcgaa gttcacgatg tccatgaccc
    19081 cgaagatcat cgtcaggccg atcgcgacga gcgcgtagac gaggccgatg agcacgcccg
    19141 aggcgagcgt ctgcaggagg atctccgcgg tcatggctgg acctcgcggt ggtggtccgc
    19201 gcgcgcgcgc atgcggggac gcgcgcggcc ggaggacggg atggtgctca tggcttgaag
    19261 ctggcgacgg tggcgaccgg ggcggcgggc gggcttactt gcgcttgtcc caggcgggca
    19321 tcggccagac gacgtcgcgc gaggcgacgg aggtcggcca gacggtgtgg tacttcccgt
    19381 cgatgatctg cacgaggatg ccggaggcct cgacgttctg cccgttctcg tcgaacttca
    19441 cgcccttcca cggcatgatc aggtggttgg cggggatgtc ggtcgacgcg agcgccttgc
    19501 ggatcgcctc ggcgtcggtc gagcccgcgc ggttgatcgc gtcggccatc accatcaggc
    19561 ccgtgaacgt gcgcgcggag ttgccgttga agtccgcctt gtagcggctg cggaagaggt
    19621 cgtcgacctg cttgatcagc gggttcttgt tggcgaggtc gagcgcccag acctcgcgcg
    19681 agatgatgta gtcggcgtcc ttgcccagcg agcgcgtgaa ctcggtgtcg gcgaagccgg
    19741 cgtcgttggc caggatcatg tccggcgtga agccgagctg cttgtacgtc ctcaccgaca
    19801 gcagcgcgtc gccgaggtag gacgactgca gcagcaccgc gggcgcggcg gccttgagcg
    19861 tctgcacctc cgaggtgagc tgcgtgctct tggccgggta ggtgacctgc ttgacgaggt
    19921 tgaagccgtc ggccggcgcg aggctcgcct cgagcttggt cgtctcgttg ccccacagcg
    19981 tgttctcgtt gaacgtcgcg atcgagggga tcttgatgcc cttcttcgcc tgcatctcct
    20041 tcatgaactc gaagaagttg cgcacgaaca ggtcgtcgtg cgcggtggtg cggaagaagt
    20101 acttgaagcc gcgctgcgtc agcgtcgccg acgacgactc ggggttgacg aacgggatct
    20161 tgtagcgctc ggccacctgg ctcgcggtgg cggtgacgtt gctgttgtac gcgccgatga
    20221 tgccgacgac gtgctcctgc gtgatcaggc gctcggcctc cgtggcgccg acctgcggcg
    20281 tgccctggtg gtcggcgaag accagcttga tcttcgcgcc gtgcagcccg ggcaggcccc
    20341 cgccggccga gaacggcagg tcgggcatgc ccttggcgcc gttgttgatg atgtcggccg
    20401 cgagctcgag cgcgtccttc atctccgccc cgctcgacgc ggccgcgccg gtcagcggat
    20461 agatcacacc gatcttgacc tcggtctggg cgtgggccgc cgtgccgagc cagaacgcgg
    20521 ccagggccgc cgtcaccttc actcgcgaaa acgcagtcat cggaacgctc ctcttgctct
    20581 tgggggtgaa acgatgacgg gattgttggc cctgtgcgcg gcgtgggcaa tcagaaatta
    20641 gtttttggtc gtcatcgact ttttagatgg cactgcggcg gtgcgacgct gcaccttcgt
    20701 ggacggcacc ggcgtggatt tcttctcggt ttgcgcgatg aaggcgagcg ccgagcgcac
    20761 gatcgcctcc gcggagtggc cggcgccgtc gggccggtag ctcgcgaaga cgggcagcgg
    20821 cggcagcggc gggtcggtgg tcagcaggcg caggtcgcgg ttggcgagca gccgcttggc
    20881 cgcggcgcgc ggcagcgtcg cgacgccgaa gccgccctcg gcgagctgcg tcatcgccga
    20941 gatcgaggac atcgcatgca cctgcttcgg ctcgagccgc gcgttgcgga acaggtcgag
    21001 cagcgcgacg tgcggatgcg agccgcgctg gaaggtcagc aggtcgtagg cggcgagctc
    21061 ctcgagcgag cagcgcgcgc gccggtgcac ctgcgcgttg ccgacgaaca ccatctccat
    21121 cgtgggcagc gcgcgcgagc gcacgccctc gccggtcgcg ggcatcgcgc agaacgcgat
    21181 gtccagcacg ccgcggcgca tctgctcggt cagcatgggg gtggtctcga cggtgagctc
    21241 cagcgcaagc gacggccgct cggcgcgcag tccctcgacc cacgggatca gccacgaatg
    21301 cagcaccgac tcgatcgcgc cgatgcgcag cggcaccgac tcgcccgcgc cggtgccgag
    21361 ctcggccttc gcctcgcgct gcagctccag caggcgctgc gcgtgcacga ggaagcgcac
    21421 gccggggatg gtgaggccga actggcggtt gctgcgatcg agcagcatga ccccgagctc
    21481 ggactcgagg gcggcgatgc ggctggacat cgccgattgc gtgatgctga gcttctccgc
    21541 ggcgcgggtg acgctcttga gggtcgcgac ccagtagaag gcttcgacga atcgcaggtt
    21601 catggcgcgc gggctccagc tgggccgtgg ggagggcgag gccgaactct agggccgaac
    21661 gccgcttgcg cgcgagcccc gcgtgagccg ggtgacgcgc gcgcgccgca cgtcgcccgg
    21721 gcgttgcatg gatccattcg cgaattgcat gacgtcctac gcggggccgg ttacggcgga
    21781 attacactgg tcgctcgccc tgctctccca tgtgtctgcg ggttaacccg cgagtccggg
    21841 acgttccctc gatgtaacgc gtcgaggcac gcggcagcgg cagcgaccgc gggattgggc
    21901 gaaccccgag gccactcgac gtggcttcga ggctcgccca atcttgtctt cgtaccttga
    21961 tgattggagt cccgtcatga accgtcccgc gatggaaggc ttgcgcctga acgtgcccgc
    22021 ctgggtcaag cacgagcgcc tggtgtcctg ggtggccgag atcgccaccc tcacgcaggc
    22081 cgccgacgtg cactggtgcg acggcacgca ggaggaatac gaccgcctgt gcgcgcagct
    22141 cgtggccgcc ggcaccttca agaagctgaa cccggccagg cgcgcgaaca gctacctcgc
    22201 ctgcagcgac ccgagcgacg tggcgcgcgt cgaggatcgc accttcatct gcagcgagac
    22261 gaaggaaggc gccggcccga ccaacaactg ggaatcgccc gagaagatgc gcgccctgct
    22321 gcagcacggc caggccgacg gcacgccgcc gctgttcgcc ggcgcgatgc gcggccgcac
    22381 gatgtacgtg gtgccgttct cgatggggcc gctcggctct ccgatctcgc acatcggcgt
    22441 cgagctctcc gacagcccgt acgtggccgt caacatgcgc ctgatgacgc gcatgggcag
    22501 gggtgccctc gacgtgctgg gcgccgacgg cgacttcgtg ccctgcgtgc acacggtcgg
    22561 cgcgccgctc gagccggggc agaaggacgt cgcctggccg tgcaacaaga ccaagtacat
    22621 cgtccactac cccgaatcgc gcgagatctg gagctacggc tccggctacg gcggcaacgc
    22681 gctgctgggc aagaagtgct tcgccctgcg catcgcctcc aacatgggcc gtgacgaggg
    22741 ctggctcgcc gagcacatgc tggtgctggg cgtgacgagc cccgagggca agaagcacca
    22801 catcgccgcc gcgttcccga gcgcctgcgg caagaccaac ttcgcgatgc tgatcccgcc
    22861 ggcgggcatg ccgggctgga aggtcacgac gatcggcgac gacatcgcct ggatcaagcc
    22921 gagcgccgac ggccgcctga tggcgatcaa ccccgaggcc ggctacttcg gcgtcgcccc
    22981 cggcaccaac accctgacca acccgaactg catggccagc ctgacgcgcg acgtgatctt
    23041 cacgaacgtc gcgctgaccg acgacggcga cgtctggtgg gaaggcatga ccgagacgcc
    23101 gcccgcgcac tgcatcgact ggcagggccg cgactggacg cccgcgatcg ccaaggagac
    23161 gggcgcgaag gccgcgcatc cgaacgcccg cttcaccgtc gccgcgacca acaacccggc
    23221 gctcgacccg gcctgggacg acccgaaggg cgtcgcgatc gacgcgttca tcttcggcgg
    23281 ccgtcgctcg accaccgtgc cgctggtgac cgaggcgcgc gactgggtcg acggcgtcta
    23341 catggccgcg accatgggct cggagaccac cgccgcggcc gtcggccagc agggcgtcgt
    23401 gcgccgcgat ccgttcgcca tgctgccgtt caccggctac aacatgagcg actacttcca
    23461 gcactggctc aacgtcggcg agcgcctgga ggcgcagggc gcgacgctgc cgaagatctt
    23521 ctgcgtcaac tggttccgca agggcgcgga cggcaagttc gtctggcccg gctacggcga
    23581 gaacatgcgc gtgatggagt ggatgctggg ccgcatcgag ggccgcgcgc agggcaccga
    23641 gaacgtgttc ggcaccagcc cgcgctacga ggacatcaac tggacgggcc tggatttcag
    23701 ccgcgcccag ttcgacagcg tcatcggcgt cgacaaggcg gcgtggcagc aggagctcgc
    23761 gatgcacgac gagctgttca agacgctcgc ctaccacctg ccgacgcagc tggcgaccac
    23821 caagagccgc atcgccgccg cgctgggctg aggcgtcgcg gcgcgccgtc cgcccctgtc
    23881 ttcgaggccg cctgcgggcg gcctttcgca tggcggtcgc gagcgcatgc ctacactggc
    23941 ggcatgcgca tccaactgct ctcggacctc cacctcgaac gcgcgcccga ctggcagccg
    24001 ccgcggctgc cggacatcga cgtgctcgtc gtcgcgggcg acgtcggctc ctaccaggcc
    24061 ggctccgagc tcgccggcga cgacttcggg ctcgcgcgct tctcgccgcg cgcgccgggg
    24121 gcgtcgtgga agcgcacgct gtacctgccc ggcaaccacg agttcgacag cctcgactac
    24181 gacgccacgt acgcgcgcct gcgcggcctg tgcgcggcgc tgggcatcga atggctggag
    24241 aaggagacga tcgtcgcggg cggcgtgcgc ttcgtcggcg cgacgctgtg ggcggacttc
    24301 gatgcgctgg cgagctcgca ggcctcgctc ggcgcgcagc tcaaggcacg cgaaaaagcc
    24361 ttccgcgctg ccaacttcta cctgggcaag tacacgacgc tgaaggacgg cgcgccggtg
    24421 ctggccgagg gctggcgcgc gatgtcgctc gccgaccagg actggctgcg cgccgcgctg
    24481 ggccgcccct tcgccggccc gacggtggcg gtcacgcact tcgcccccag cctgcgcagc
    24541 gcggatccgc gctacggcct ggtgccgggc acggccggct tctgcaacgc gatggacgac
    24601 ttcctgccgc tcgcggactt ctggctgcac ggccacctgc attgcgccaa cgactacctg
    24661 gcgagcggca cccgcccgga cgggacgccg tggtcgtgcc gcgtggtggc gaacccgctg
    24721 ggctacctca agcgcggcga gcaggaggac ttccggccgg acctcgtgat cgagatcccg
    24781 tcgcgctgaa cgcgcatcag gcgccgagct tcctgcccag ccacatccgc cacggcgcca
    24841 cgtgcacgac cgggcgcggc gcggccggct cgacggccac cgcggactcg tagcggccgg
    24901 cgccgtccat cgcctcgtcg agcgcgaagg cggtgcccat gtcggccagg tccggctctc
    24961 gtcgcgcacc cagcgaacgg ccgaagtggc ggaggaacgc ggggcgcgag gcggtgggca
    25021 tggtggctct cctgatgtga tgaaccgtga agccagtgtg ttgcaacgtg tcaggaccgg
    25081 tacatgaaaa tgtgtgccga atgcccggca gttcgccttg gcgcgcgagg atgccaccgg
    25141 aaagcaacgc gcgcgtgacg gccttcacag gtgccgcgac agggccgcga acggctccgc
    25201 gacaggcccc gttcagacgc cgctgtttcc acgcgtcccc ccctccatcg agggtggcgg
    25261 ggtcttgcga acgcgcttaa tctgcaagct cgacgaccgg gccgcgagcc gcgcgcccga
    25321 cgtgcgtccg aggagccgcc atgcgcgccg taccgcttca gttgctcacc tggggaacga
    25381 tcaccgccgc ggtctcgagc gccgtgctga tgtcgtcgtc caaccagggc gccgtcttct
    25441 gctcgatcgg ctatcgcgcg acgagcgcgg cgatcggcct cgacctgtcg gacgagcccc
    25501 cgcgcgccgg cccgacggtg gacaagcccc gctcgacggc cggcaaggac gcgtcggccg
    25561 tgtgagtcgg gcccgccgca acgttgcgat cagctggcgt tcatcacatc cactaatgaa
    25621 gccggatttc gattaatccg gcttctttca tttcagggtc gtccggaaaa taccgaagag
    25681 cgtggaaacg ccgcgggatg cgcctcgccg aggccgcgcg gcgtctcgcc gcacgaggac
    25741 ccgatgaaag tgatcgttct gggcgccggc atcatcggcg tcgccaccgc ctggtacctg
    25801 ctcgaggagg gccacgaggt gaccgtcgtc gaccgccagc cggacgcggc cctcgagacg
    25861 agcttcgcca acggcgcaca gatctccgtg tgcttctgcg agccctgggc gaatgcgagc
    25921 gccccggcca aggtcgcgaa gtggatgctg caggacgact cgccgctgct gttccgcccg
    25981 cgcctcgacc cgcaccagtg gcgctgggcg ctgtccttcc tcacgcagtg caccgatgcg
    26041 gccttcgcgc gcaacgtgtc gcagctggtc gcgctcggcc gctacagcca cgagtcgctg
    26101 aagacgctcg tcgagcgcac cggcatctcc tacaaccggc tagagcgcgg catcctccac
    26161 ttcttctcgt cggaggccga ctaccgctcg ggcgtcgccg cggccgagct gatgcgccgc
    26221 cagggcgtcg accgccgcat cctgggccgc gacgaggtgc tggccgtcga gcccgcgctc
    26281 aaggccttcg ccgccaacat ccacggcggg acgttcacgc ccagcgacga gtccggcgac
    26341 gcgcgcgtct tcacgcagca gctcgccgcc cgctgcgcgg cgcgcggggc gacgttcctg
    26401 tggggccacg acgtcgccgg cttcgagcgc cgcgacgagc tgctggaagg cgtggtcgtc
    26461 cacgagcgcg cgaccggcgc ccgccgcacg ctgcgcagcg accaggtcgt cgcggcgacg
    26521 ggcccgttca ccgcgccgct gctgcgccag ctgggcatct ggctgaacat ctatccggcc
    26581 aagggctact cggcgacgat gaagctcaag cgccccgagg acgccagcgt ggtcagcctg
    26641 ctggacgaca cgcgcaagat cgcgatctcg cgcctgggcg accacatccg catcgccggc
    26701 accgccgaga tggccggcta cgacacctcg atcgacggcg cgacctcgcg tcgccgctgc
    26761 gaggcgctgg tgcggcgcta cgagcagctg ttccccggcg tcgccgacac gagcgagccc
    26821 aacttctggg ccggcctgcg ccccagcacg cccaacaatc tgccctacat cggccgcagc
    26881 cgcatcgcca acctgtggct caacgccggc cacggcacgc tgggctggac gcacggcgcc
    26941 ggctcgggcc gcgcggtcgc cgagctgatc agcggccgcc gcccgcagct ggactacggg
    27001 ttctgcggcg acgcgctgcc cgtgcgccgg ccccgcg
//
LOCUS       DASVLN010000024         3716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_484581, whole genome shotgun sequence.
ACCESSION   DASVLN010000024 DASVLN010000000
VERSION     DASVLN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484581"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="VF453_00670"
     CDS             <1..145
                     /locus_tag="VF453_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX7686179.1"
                     /translation="DASITRLVGAMMLEQNDEWSLNRRYMTLEGLQTLSDTPRTRLPA
                     VAR"
     gene            complement(578..1609)
                     /locus_tag="VF453_00675"
     CDS             complement(578..1609)
                     /locus_tag="VF453_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX7686180.1"
                     /translation="MSTIPGVSVPRAVGAALGLSVFALASLHAAAADVASIPLPDPVR
                     WDYVSVDAPAHRLYVAHRARVDVVDIITGKPVLQLSPTPGVHGAAAAADVGRVFTSNG
                     GEDAVGVFDIASGREVGKVKVGHGPDAIVYEPVTHRVLAFNGHSSDVTAIDAASLRVL
                     AASIPSPGRPEFAVVDGHGRVYFNIEDKNELAVLDARTLKVERHYSLAPCDSPSGLAI
                     DPGGRLYSVCENGMLVVSDPTQGKVIGQAAIGHGPDGVAWMDGKAWSANGQDGTISVV
                     SETAPGHFESVATLQTARGARTIAADPSLHRLFTPTADFKPQTASDAGKKRPEAVPGS
                     FRVLVVSTR"
     gene            complement(1609..2040)
                     /locus_tag="VF453_00680"
     CDS             complement(1609..2040)
                     /locus_tag="VF453_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686181.1"
                     /translation="MARWLAAASCAVATAVHAGPPFLTDDPEPVDLGHAEVNAIWQET
                     RTVDGRSGALSGEVNIGCARETQCHAAVPLAVDRPDAGPHRAGLGDVEAGVKYRFLVG
                     FNLGLIAEVAPHLNLLLSAGRGLTNVQADRRSVFLALQREL"
     gene            complement(2093..2881)
                     /locus_tag="VF453_00685"
     CDS             complement(2093..2881)
                     /locus_tag="VF453_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686182.1"
                     /translation="MDIDRRRTAESAVAKVPAVTFAFWLIKIAATTLGETGGDWVTMS
                     LKLGYLVGTAIFAALFVGLVFLQVRSTRFHPLIYWATIVATTTLGTTMADFFDRSIGV
                     GYPGGASIVGLLLLASLFAWHRVEGTVSIASVTTPRAEWFYWVTILFSQTLGTALGDW
                     TAGDDRGGLALGYENAALLFGGALVLVAGLYFWTRTSRTLLFWIAFVLTRPLGATVGD
                     FLDKPHSAGGLELSRLYASVALLAFMALCIALLPQRPARAGHAG"
     gene            complement(3051..>3716)
                     /locus_tag="VF453_00690"
     CDS             complement(3051..>3716)
                     /locus_tag="VF453_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7686183.1"
                     /translation="LNPRKRIAQALDEPLRLNTALAPAERRERIGAMLARVGLRPQDA
                     QRWPHMFSGGQRQRIAIARAMMLAPKLLVADEPTSALDVSIQAQVLNLLMDLQAASGL
                     GLVFISHNLGVVEHLADRVMVMYLGRVVERAPRATLFARPLHPYTKALLSATPELPGA
                     VRRERIKLVGELPSRLAPPAGCALVGRCPFARERCVVEAPALRPVDEAEVACYFAEEI
                     AGG"
BASE COUNT          629 a         1288 c         1283 g          516 t
ORIGIN      
        1 cgacgcctcc atcacgcgcc tggtcggcgc catgatgctc gagcagaacg acgagtggtc
       61 gctgaaccgg cgctacatga cgctggaagg cctgcagacc ctcagcgata ctccgcgcac
      121 tcggctgccc gcagtggcgc gttgagcatg tgatcctcag cctgttcggg taagcgactt
      181 acaccacggc ttgggacatc accgcagcct gcccaaaaga ggtgcggcag ggacgatctt
      241 gcgtagaagg caaccactgc gccggtagcg agcaggacgg tgccttggac gcacaagaaa
      301 aaccttatcg ctcttacagg cggcttgcgc tcaatccagg gctggagcag agcccagagg
      361 acgttcatgg cgagcatgcc cagaaataag accgaaagga tcttcagcac atgttccatg
      421 gttcagcaga aagcctaccg caaagccggc agacgtcgaa gtccgcatcc ggccgccatt
      481 cgccgatcgc ccggcttggc ggggctgagg ttcgcccacg tcttcattct ggacgagcta
      541 ccctcgcccc cgccggtcgc gggccgacgt ggcacgctca acgggtgctg acaacgagga
      601 ctcggaaaga gcccgggacg gcctcgggcc gctttttgcc ggcgtcgctg gccgtctgcg
      661 gcttgaagtc cgctgtcggg gtgaacagcc ggtgcaacga cgggtcggcg gcgatcgtgc
      721 gggcaccgcg cgcggtttga agcgtggcca cggactcgaa gtgccccggc gccgtttcgc
      781 tcacgacgct gatcgtgccg tcctggccgt tggcgctcca cgccttgccg tccatccagg
      841 cgacgccgtc cggcccgtgg ccgatcgcgg cctggccgat gaccttgccc tgagtcggat
      901 cgctcaccac cagcatgccg ttctcgcaca cggagtagag gcggccgccc ggatcgatcg
      961 ccagtccgct gggactgtcg cacggcgcga ggctgtagtg gcgctcgacc ttcaacgtgc
     1021 gcgcgtcaag cacggcgagc tcgttcttgt cctcgatgtt gaagtacacg cggccgtggc
     1081 cgtcgaccac cgcgaactct ggccggcccg gactcgggat gctcgccgcc agcacgcgca
     1141 gcgaggcagc gtcgatcgcc gtcacgtccg acgaatggcc gttgaacgcc aacacacggt
     1201 gcgtgaccgg ctcgtagacg atggcgtcgg gaccgtggcc caccttcacc ttgccgacct
     1261 cgcggccgct ggcgatgtcg aagacgccga cggcgtcctc gccaccgttg ctggtgaaca
     1321 cgcgcccgac gtcggcggcc gccgccgcgc cgtggacacc cggcgtgggc gacagctgca
     1381 ggacgggctt gccggtgatg atgtcgacga cgtcgacacg cgcgcgatgc gcgacataca
     1441 ggcggtgcgc gggcgcatcg acgctgacgt agtcccagcg cacggggtcg ggcagcggga
     1501 tcgatgcgac atcggccgcc gcggcgtgca gcgacgccag cgcgaacacg gacaggccga
     1561 gggcagcgcc gactgcgcgc ggcacggaaa caccagggat cgtcgacatc agagctcccg
     1621 ttgaagggcg aggaagacgg agcggcggtc ggcttggaca ttcgtgagtc cacgccccgc
     1681 cgacaagagc aggttcaggt gtggcgccac ttccgcgatg aggcccaggt tgaatccgac
     1741 gaggaagcga tacttgacgc ccgcttcaac gtcgccaaga cctgcgcgat gcggacccgc
     1801 gtcggggcga tcgactgcga gcggcaccgc cgcatggcac tgggtctcgc gagcacagcc
     1861 gatgttgact tcgccgctaa gtgcgccgga gcgcccgtcc actgtgcgcg tctcctgcca
     1921 gatcgcgttg acctcggcat ggcccaggtc gacgggctcg ggatcgtccg tgaggaacgg
     1981 tggcccggcg tgcacggcag tcgccacagc gcaggaggcg gccgcgagcc agcgcgccac
     2041 gccggatcgc ctgagccccc gcgccccgcc agccgcggag acgctcgacc cgtcagccgg
     2101 catgcccggc ccgcgcgggc cgttgcggca gcagcgcgat gcacagtgcc atgaaggcca
     2161 gcagcgccac cgacgcgtag agccggctga gctccaggcc gccggcagag tgcggcttgt
     2221 cgaggaagtc gccgaccgtc gcgcccagtg gccgggtgag gacgaaggcg atccagaaca
     2281 gcagcgtgcg cgacgtgcgt gtccagaagt acaagccggc gaccagcacc agggccccgc
     2341 cgaacagcag cgccgcgttc tcgtagccca gcgcgaggcc gccccggtcg tcgcccgccg
     2401 tccagtcgcc cagtgcggtg cccagcgtct gcgagaacag gatcgtcacc cagtagaacc
     2461 actcggcgcg cggcgtggtg acgctggcga tcgacaccgt gccttccacg cgatgccatg
     2521 cgaacaggct ggcaagcagc agcagtccga cgatgctggc gccaccggga tagcccacgc
     2581 cgatcgaccg gtcgaagaag tcggccatgg tcgtgcccag ggtcgtggtc gcgacaatgg
     2641 tcgcccaata gatgagcgga tggaagcgcg tcgagcgcac ctgcaggaag acgagcccca
     2701 cgaacagggc tgcgaagatg gccgtgccca cgaggtagcc gagcttcagc gacatcgtca
     2761 cccagtcgcc gcctgtctcg cccagcgtcg tggcggcgat cttgatgagc cagaaggcaa
     2821 atgtgaccgc cggcaccttg gccacggcgc tttcggcggt acgtcgtcga tcgatgtcca
     2881 atgggtgctc ctgcaagatc gcccggcccg cttggtcggg cacacctcga acccgctgaa
     2941 tccgctgagc cgcgcatcaa ttcggcggca catagtctag agcttgtgtc cgacgagctc
     3001 gggcaaagac cgttccggcg aggcgcgaag ttcctatgac aaccacgagc tcaaccgccc
     3061 gcgatctcct cggcgaagta gcacgccacc tccgcctcgt caacgggccg caacgccggc
     3121 gcctcgacga cgcaccgctc ccgcgcgaac ggacaccgcc ccaccagcgc gcaccccgcc
     3181 ggcggcgcga gccgcgacgg cagttcgccc accagcttga tgcgctcgcg ccgcaccgct
     3241 ccgggcagtt ccggcgtcgc cgacagcagc gccttcgtgt agggatgcag cggccgcgcg
     3301 aacagcgtcg cgcgcggcgc gcgttcgacg acgcggccga ggtacatcac catcacgcgg
     3361 tcggcgaggt gctcgacgac gcccaggttg tgcgagatga agacgaggcc gaggccgctg
     3421 gcggcctgga ggtccatcag gaggttgagc acctgggcct ggatcgacac gtcgagcgcg
     3481 gaggtcggct cgtcggcgac cagcagcttc ggcgcgagca tcatggcgcg cgcgatcgcg
     3541 atgcgctggc gctggccgcc cgagaacatg tgcggccagc gctgcgcgtc ctgcgggcgc
     3601 aggccgacgc gtgccagcat cgcgccgatg cgctcgcggc gctcggcggg cgcgagcgcg
     3661 gtgttcaggc gcagcggctc gtcgagcgcc tgcgcgatgc gcttgcgcgg gttgag
//
LOCUS       DASVLN010000025         4871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_270317, whole genome shotgun sequence.
ACCESSION   DASVLN010000025 DASVLN010000000
VERSION     DASVLN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270317"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1115
                     /locus_tag="VF453_00695"
     CDS             219..1115
                     /locus_tag="VF453_00695"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7686184.1"
                     /translation="MKQIGMVGIGLMGHGIASNLARHGWPLTVLEHPGNQPLDRLRLA
                     GVRTESSAARLAAGADVVIVCVTGSPQVEAVLLGEDGVLAGLRPGAIVVDCSTAIPSS
                     TARIAAAVRAAGGRFIDAPMTRTPKEAAQGRLNLLVGGDVADVEECRPLLACFAENIV
                     HAGGVGAGHRMKLLHNYVSLGSVALLAEAAACARRAGIDASVLVEVLAAGGGGGTALE
                     RLRPALLAGDTSALKFAMANARKDLGYYTEMAGETHAADRIAGAVLRTFDAGVEAGGP
                     QAMVLELVELLAGHGDAPPFGS"
     gene            1140..2246
                     /locus_tag="VF453_00700"
     CDS             1140..2246
                     /locus_tag="VF453_00700"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX7686185.1"
                     /translation="MNAPHRIAVIPGDGIGHEVVPEGLRVLQAAAARFDIALAFDHFD
                     DWCTAHWLKHGTMLPADWKAQVGGHEALFFGAVGAPDVVPDHVALWESLILFRREFEQ
                     YVNLRPCRLMGGVPSPLANRRPGDIDFVVVRENTEGEYSSIGGRAFAGTEREFAIQET
                     VFTRVGVDRILRYAFELARARPRKDLVAATKSNGISITMPYWDERLAEMARQYPDVKV
                     RKYHVDILCAHFVQHPDWFDVVVGSNLFGDILSDLGPACTGTIGIAPSANLNPERRFP
                     SLFEPVHGSAPDIAGQGIANPIGQIWSGALMLEHLGRGDARYQAAADAIVDAIEDVLS
                     SGPRTRDMGGTATTVEVGAAIAARVATIPARHAD"
     gene            2258..3709
                     /locus_tag="VF453_00705"
     CDS             2258..3709
                     /locus_tag="VF453_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7686186.1"
                     /translation="MQPPRHWIANEWVAPANGETIPVLDPSTGETFAELARGDAGDVD
                     AAVRAASAAMGEAFEGPWGRTPAAQRGRVLARLAALVLEHAEEIARLEARDTGKAVRT
                     ARNDVAALARYFEYYAGAADKLHGETLPYESGYTVLTIREPHGVTGHVIPWNYPIQIF
                     GRSVGASLAAGNACVVKPAEDASLSVLLLARLGAEAGLPAGVLNVVTGYGQEAGAALT
                     AHPQVAHLSFTGSTATGRLVAEAAARRHVPAVLELGGKSPQLVFADADLDAALPVLVN
                     AIAQNAGQTCSAGSRLLVERTAYEPLLERLAQRFAALRAGPSSADRDMGPLVNRKQLE
                     RVRGFVAQAAQDGVVAMARGTVDADAPAGGFYHEAVLFRDVPPGHALAREEVFGPVFA
                     AMPFDDEDEALALANGTDYGLVAGIWTRDGARQLRLARRLHCGQVFVNNYGAGGGVEL
                     PFGGVKSSGHGREKGFEALAGFTRLKTIAIRHG"
     gene            4053..>4871
                     /locus_tag="VF453_00710"
     CDS             4053..>4871
                     /locus_tag="VF453_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX7686187.1"
                     /translation="MQRLLALLLAFAPAFVLAQDLAAAPTPAPAAASAPATWNAHFQA
                     TYVRQWQPAFAAAVDGPHSLRNAAGASWSFTATAFLGLRLGEATELHLDPEVAAGLPL
                     SGLAGLAAFPDGELAKTSGERSKPYVARLFLRHVVNLGGAPVAVEDDTDQLAGTVDAH
                     RLVVTAGRLSALDLFDANPVAHDPRTQFLNWALMTHAAYDYPADARGYTDGLALEVDD
                     GGWSLRAARFAEPRVPNALALDGSLLKRHGDQLELVRAWDVGGRAGDARLLAFRA"
BASE COUNT          617 a         1880 c         1743 g          631 t
ORIGIN      
        1 gacgagcagg ccattggcac gtggcgtgcc tcggcgcggc gctgcggcat tcgcccgcgt
       61 cgttgaaaga aggccgcgct gctgcaatga tgtcggcggc gcctgcggca agccctgccg
      121 ccggcaccgc gatccggccg caagccacgg cgcaccgcgg ccgcccggag acgagcgccg
      181 gcatgcgcct ggaggcagcg ccggcagggg acgcacgcat gaagcagatc ggcatggtgg
      241 gcatcggcct catgggccac ggcatcgcca gcaaccttgc gcgccacggc tggccgctga
      301 cggtgctcga acatccgggc aaccagccgc tcgaccgcct gcgcctggcc ggcgtgcgca
      361 ccgagtccag cgccgcccgc ctggccgcgg gcgccgacgt cgtcatcgtc tgcgtgaccg
      421 gctcgccgca ggtcgaggcc gtgctgctgg gcgaggacgg cgtgctcgcc ggcctgcgac
      481 cgggcgcgat cgtcgtcgac tgctcgaccg cgatcccgtc gtcgaccgcg cgcatcgccg
      541 ccgccgtgcg cgccgcgggc ggccgcttca tcgacgcgcc gatgacgcgc acgcccaagg
      601 aggcggcgca gggccggctg aacctgctcg tgggcggcga cgtcgcggac gtcgaggaat
      661 gccggccgct gctggcctgc ttcgccgaga acatcgtgca tgcgggcggc gtcggcgcgg
      721 ggcatcgcat gaagctgctg cacaactatg tctcgctcgg cagcgtcgcg ctgctggccg
      781 aggccgcggc ctgcgcgcgg cgtgcgggca tcgacgcgtc ggtgctggtg gaggtgctcg
      841 ccgcgggtgg cgggggcggc accgcgctcg agcgcctgcg ccccgcgctg ctggcgggcg
      901 acacctcggc gctgaagttc gcgatggcca acgcacgcaa ggatctcggc tactacacgg
      961 agatggccgg cgagacgcac gcggcagacc gcatcgccgg cgcggtgctg cgcaccttcg
     1021 acgccggcgt cgaggccggc gggccgcagg cgatggtgct cgagctcgtc gagctgctcg
     1081 ccgggcacgg cgacgccccg cctttcggaa gctgaaccca cacctcaccc cgtatcgaca
     1141 tgaatgctcc ccaccgcatc gccgtcatcc ccggcgacgg catcggccac gaggtcgtgc
     1201 ccgagggcct gcgcgtcctt caggccgcgg ccgcgcgctt cgacatcgcg ctcgccttcg
     1261 accatttcga cgactggtgc accgcgcact ggctgaagca cggcacgatg ctgccggccg
     1321 actggaaggc gcaggtcggc ggccacgagg cgctgttctt cggcgcggtg ggcgcgcccg
     1381 acgtcgtgcc cgaccacgtg gcgctgtggg agtcgctgat cctgttccgg cgcgagttcg
     1441 agcagtacgt caacctgcgg ccgtgcaggc tgatgggcgg cgtgccgtcg cccctcgcga
     1501 accgccggcc cggcgacatc gacttcgtcg tcgtgcgcga gaacaccgag ggcgagtact
     1561 cgtcgatcgg cggccgcgcc ttcgccggca ccgagcgcga gttcgcgatc caggagacgg
     1621 tcttcacgcg cgtcggcgtc gaccgcatcc tgcgctacgc cttcgagctc gcgcgcgcgc
     1681 ggccgcgcaa ggacctcgtc gccgcgacga agtccaacgg catctcgatc accatgccct
     1741 actgggacga gcgcctcgcg gagatggcgc ggcagtatcc cgacgtgaag gtgcgcaagt
     1801 accacgtcga catcctgtgc gcgcacttcg tgcagcaccc ggactggttc gacgtcgtcg
     1861 tcggctccaa cctgttcggc gacatcctgt ccgacctcgg acccgcctgc accggcacga
     1921 tcggcatcgc gccgtcggcc aacctcaacc cggagcgccg cttcccgtcg ctgttcgagc
     1981 ccgtgcacgg ctcggcgccg gacatcgcgg ggcagggcat cgccaacccc atcgggcaga
     2041 tctggtcggg cgcgctgatg ctcgagcacc tgggccgcgg cgacgcgcgc taccaggccg
     2101 cggccgacgc gatcgtcgac gccatcgagg acgtcctctc gtcgggcccg cgcacgcgcg
     2161 acatgggcgg caccgccacg accgtcgagg tcggcgcggc gatcgccgca cgcgtcgcca
     2221 cgattcccgc gcgacacgcc gactaggaga ccgccgcatg cagccgcccc gccactggat
     2281 cgccaacgag tgggtcgctc ccgcgaacgg cgagaccatc cccgtcctcg acccgagcac
     2341 cggcgagacc ttcgccgagc tcgcccgcgg cgacgccggc gacgtcgatg ccgccgtgcg
     2401 cgcggcctcg gccgcgatgg gcgaggcctt cgagggcccg tggggccgca cgccggcggc
     2461 gcagcgcgga cgggtgctgg cgcggctcgc ggcgctggtg ctcgaacacg ccgaggagat
     2521 cgcccggctc gaggcgcgcg acaccggcaa ggcggtgcgc accgcacgca acgacgtcgc
     2581 ggcgctggcg cgctacttcg agtactacgc cggcgcggcc gacaagctgc acggcgagac
     2641 gctgccctac gagtccggct acacggtgct gacgatccgc gagccgcacg gcgtcaccgg
     2701 ccacgtcatc ccgtggaact acccgatcca gatcttcggg cgcagcgtcg gcgcctcgct
     2761 cgcggcgggc aacgcctgcg tggtcaagcc ggcggaggac gcgagcctgt cggtgctgct
     2821 gctcgcgcga ctcggcgccg aggccggcct tcccgcgggc gtgctcaacg tcgtgaccgg
     2881 ctacggccag gaggccggcg ccgcgctgac ggcgcatcca caggtcgccc acctgtcgtt
     2941 caccggctcg acggcgaccg ggcgcctcgt cgccgaggcc gcggcccggc gccacgtgcc
     3001 cgcggtgctg gagctgggag gcaagtcgcc gcagctcgtg ttcgcggacg ccgacctcga
     3061 cgccgcgctg ccggtgctcg tcaacgcgat cgcccagaac gccggccaga cctgctccgc
     3121 gggcagccgg ctgctggtgg agcgcacggc gtacgagccg ctgctcgagc gcctcgcgca
     3181 gcgcttcgcc gcgttgcgcg ccgggccgtc gtcggccgac cgcgacatgg ggccgctcgt
     3241 caaccgcaag cagctcgagc gggtgcgcgg cttcgtggcg caggccgcgc aagacggcgt
     3301 cgtggcgatg gcgcgcggca cggtcgacgc cgacgcgccc gcgggcggct tctaccacga
     3361 ggcggtgctg ttccgcgacg tgccgcccgg gcacgcgctc gcgcgcgagg aggtcttcgg
     3421 cccggtgttc gcggccatgc ccttcgacga cgaggacgag gcgctcgcgc tggccaacgg
     3481 caccgactac ggcctcgtcg cgggcatctg gacgcgcgac ggcgcgcgcc agttgcgcct
     3541 cgcgcggcgg ctgcattgcg ggcaggtctt cgtcaacaac tacggcgcgg gcggcggcgt
     3601 ggagctgccg ttcggcggcg tgaagtccag cggccacggc cgcgagaagg gcttcgaggc
     3661 gctcgcgggc ttcacccgcc tgaagaccat cgcgatccgc cacggctgat ccgccgccga
     3721 cagcgtcgcg tcagaccgtg cacgggccgg tgtcaggcgc cggccggcat gtttgaccgg
     3781 cgcgcccggc cgacgataat ccgcggcttc cacaacgacg tcgcccgacc atggaatccc
     3841 atcccagtcg ccggcctccg tcgtccgcac cgcacgcctg acgccgcgct ccgcgcgcgc
     3901 cagcgaggaa ggatcggacg cagaaggccg cccgcatccc gcttcctcgc cgcccggcat
     3961 cccgttcgcc gggccttccg catcctcgac gcgccgcccc ttgccgcgcg tcctcgtccg
     4021 gccgcgatcg cgcgcgccgg agggagtccc ccgtgcaacg ccttctcgcc ctcctgctgg
     4081 ccttcgcgcc ggccttcgtc ctcgcccagg acctcgcggc cgcaccgacg cccgcgccgg
     4141 ccgcggcctc ggcccccgcc acctggaacg cgcacttcca ggccacttac gtgcgccagt
     4201 ggcagccggc cttcgcggcg gcggtcgacg gcccgcacag cctgcgcaac gccgccggcg
     4261 cgagctggtc gttcaccgcc acggccttcc tcgggctgcg gctgggcgag gcgaccgagc
     4321 tccacctcga tccggaggtc gcggccggcc tgccgctgtc gggcctggcc ggcctggccg
     4381 cgttccccga cggcgagctc gccaagacct cgggcgagcg gtccaagccc tacgtcgcaa
     4441 gactgttcct gcgccacgtc gtgaacctcg gcggcgcgcc cgtcgccgtc gaggacgaca
     4501 ccgaccagct tgccggcacg gtcgacgcgc accgcctggt cgtcacggcg ggccggctgt
     4561 cggcgctcga cctgttcgat gccaacccgg tcgcccacga tccgcgcacg cagttcctga
     4621 actgggcgct gatgacgcac gcggcctacg actatcccgc cgacgcgcgc ggctacaccg
     4681 acggcctcgc gctggaagtc gacgatggcg ggtggagcct gcgcgccgcg cgcttcgccg
     4741 agccgcgcgt gccgaacgcg ctcgcgctcg acgggtcgct gctgaagcgc cacggcgacc
     4801 agctcgagct cgtgcgcgcc tgggatgtcg gcggccgcgc gggcgatgcg cgcctgctcg
     4861 cgttccgcgc c
//
LOCUS       DASVLN010000026         4371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_216160, whole genome shotgun sequence.
ACCESSION   DASVLN010000026 DASVLN010000000
VERSION     DASVLN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216160"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF453_00715"
     CDS             <1..241
                     /locus_tag="VF453_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX7686188.1"
                     /translation="SAQVALDADGQARLDALKSWRAEVAREHNLPAYIVFHDATLAQM
                     AAQAPSTLDQLAGISGVGAKKLEAYGREILRVLGG"
     gene            338..1114
                     /locus_tag="VF453_00720"
     CDS             338..1114
                     /locus_tag="VF453_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide transhydrogenase"
                     /protein_id="HEX7686189.1"
                     /translation="MPDALIGATGFVGTTLLKSAAFDARYRSTDIGEIEGRRFGTVVC
                     AGAPAQKWIANREPEADRAGIDKLIGHLDRVECDTFVLVSTVDVFGSPVAVDEDSAVV
                     TDGLHAYGRHRFLLEEFVRRRFARHLVARLPGLVGPGLRKNAIHDLHHGHEVAKIDSR
                     GVFQFYPMVNLWYDLQAALAAGLSLVHLTAEPVSVADVARLGFGRPFDNPVAPAPARY
                     DMRSRHAAVFGGRDGYQYSARETIQAVRHYAQSEPARAKG"
     gene            1122..1946
                     /locus_tag="VF453_00725"
     CDS             1122..1946
                     /locus_tag="VF453_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX7686190.1"
                     /translation="MRIALSNIAWDRPEDADVAALLRRHGLDAVDLVASKYFPDAAAA
                     TDAQVRAIRAWWADQGLEVTGMQALLFGTTGLALFGDAAARAAMLRHLGHVCRIGGGL
                     GARALVFGSPRNRDRGTLADAEVEAIADPFFRALGDVAADHGVVVCLEPNPPHYGGTF
                     MTTSAETLAVVARVAHPAIRMQFDTGALTISGESPDEVLASAAPWIAHVHASEPDLLP
                     LGDGRTDHAAMAAALSRHLADRVVAIEMLATRAEPHLAAIERAVVVARRFYGDAAR"
     gene            2025..2477
                     /locus_tag="VF453_00730"
     CDS             2025..2477
                     /locus_tag="VF453_00730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7686191.1"
                     /translation="MTDPWTIRPVAPDDAARVAAHRGFAPTDAEGPAGYAAWLRPRIA
                     SGAYVGRLAIAADGAVVGGAGAVLLDWGPTRGNPGGTMARIVNVFTDEPWRRRGIARA
                     LVQAVLARCEEAGVREFNLGATPEAAALYASVGFGPYSAEMRRRVPSA"
     gene            complement(2573..3133)
                     /locus_tag="VF453_00735"
     CDS             complement(2573..3133)
                     /locus_tag="VF453_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEX7686192.1"
                     /translation="MALKATIHKVRLQLADMDRNLYGDHDLTIARHPSETDERMLVRV
                     LAYALNVPADDERGRLELAKSLWDTDEPDLWQVDLTGQVVQWIELGQPDERRLSKAAS
                     RAERVTVYSYASSTPIWWQGLQGKLVRAPKVEVWSIAPESSQALAALVERTMAWQVTV
                     QDGTVWVSDTKNTVEITPVRLKAREG"
     gene            complement(3176..>4371)
                     /locus_tag="VF453_00740"
     CDS             complement(3176..>4371)
                     /locus_tag="VF453_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7686193.1"
                     /translation="SSEHRGVRLRSHFQPIYSLTHQRVVGHEALLRADDLATGMAVAP
                     QALFDACADDAARVDLDRAAVLQHLRAHAAGPHPEWLFLNVHPRTLGSPLADRLGEIG
                     AAFAAHGLRPEQVVIEVLESSMPDDAAFDRRLDALRGLGCLVSLDDFGAGHSNFDRVF
                     RLRPEIVKLDRSVVVRAEGDAHALRIAAQMVSLLHECGCLVLMEGIETAEGADIALRC
                     DVDFVQGWHFGLPAPGRAAGRTGLHALQAAWDRFDRQSSTDDQAWQARMTPYRQAIEL
                     ASVLLAGGATLEEACRRFLALPGADMCYLLDRRGRQVVTNAIGAGSPQQQLEGLARFA
                     PLHDTREARWSRRAYFRSAQRQPNIAQVTRPYMTLQGRRMCVTVSIQFERAGEPLILC
                     GDAAW"
BASE COUNT          551 a         1614 c         1622 g          584 t
ORIGIN      
        1 ctcggcgcag gtggcgctgg acgccgacgg ccaggcccgc ctcgacgcgc tgaagtcctg
       61 gcgcgccgag gtcgcgcgcg agcacaacct gcccgcctac atcgtcttcc acgacgcaac
      121 gctcgcgcag atggcggcgc aggcgccgtc cacgctggac cagctcgccg gcatcagcgg
      181 cgtcggcgcg aagaagctcg aggcctacgg ccgcgagatc ctgcgcgtgc tcggcggctg
      241 agcgcgcctc gccggacgcc gccatgccgc gcgacaccgt cgccccgcgc gcccgagtgg
      301 gcgcccccgg cccgaacggc cttccggaga tccgcccatg cccgacgccc tgatcggcgc
      361 caccggcttc gtcggcacga ccctgctgaa gtcggccgcc ttcgacgccc gctaccgctc
      421 gaccgacatc ggggagatcg aaggacgccg cttcggcacc gtcgtctgcg ccggtgcgcc
      481 cgcgcagaag tggatcgcca accgcgagcc ggaggccgac cgggcaggta tcgacaagct
      541 gatcggccac ctcgatcgcg tcgagtgcga caccttcgtg ctcgtctcga cggtcgacgt
      601 gttcggctcg ccggtggccg tcgacgagga ctcggccgtc gtcacggacg gcctgcacgc
      661 ctacggtcgc caccgcttcc tgctcgagga gttcgtgcgc cgccgcttcg cgcgccacct
      721 ggtcgcgcgg ctgccggggc tcgtcgggcc cgggctgcgc aagaacgcga tccacgacct
      781 gcaccacggg cacgaggtcg ccaagatcga cagccgcggc gtgttccagt tctatccgat
      841 ggtcaacctc tggtacgacc tccaggcggc gctggcggcc gggctctcgc tggtgcacct
      901 gacggccgag ccggtcagcg tggccgacgt cgcgcgcctg ggcttcggac ggccgttcga
      961 caacccggtc gcgcccgcgc ccgcgcgcta cgacatgcgc agccggcacg ccgcggtctt
     1021 cggcggccgc gacggctacc agtactcggc gcgcgagacg atccaggcgg tgcggcacta
     1081 cgcccagtcc gagccggccc gggcgaaggg ctgagccacc gatgcgcatc gcgctgtcca
     1141 acatcgcctg ggatcgtccc gaggacgccg acgtcgccgc gctgctgcgc cgccacgggc
     1201 tggacgcggt cgacctcgtc gcctcgaagt acttccctga cgccgcggcc gccaccgacg
     1261 cgcaggtgcg cgcgatccgc gcctggtggg ccgaccaggg cctcgaggtg acgggcatgc
     1321 aggccctgct gttcggcacg acgggcctcg ccctgttcgg cgacgcggcg gcacgcgcgg
     1381 cgatgctgcg ccacctcggg cacgtctgcc gcatcggcgg tggactgggc gcgcgggcgc
     1441 tggtgttcgg ctcgccgcgc aaccgcgacc gcggcacgct cgccgacgcc gaggtcgagg
     1501 cgatcgccga cccgttcttc cgcgcgctgg gcgacgtcgc ggccgaccac ggggtcgtcg
     1561 tctgcctcga gccgaacccg ccccactacg gcggcacctt catgacgacc agcgcggaga
     1621 cgcttgcggt cgtcgcgcgc gtcgcccatc ccgcgatccg catgcagttc gacaccggcg
     1681 cgctgacgat cagcggcgag tcgcccgacg aggtgctcgc gtcggccgcg ccctggatcg
     1741 cccacgtgca cgcgagcgag cccgacctcc tgccgctcgg cgacgggcgc accgaccacg
     1801 cggcgatggc ggccgcgctc tcgcgccatc tcgccgaccg cgtcgtcgcg atcgagatgc
     1861 tggcgacgag ggcggagccg caccttgcgg ccatcgagcg ggccgtggtc gtggccaggc
     1921 gcttctacgg cgacgcggcg cgctgagtcc ggcgggcccc tggccgtgtc cttcggtccc
     1981 ggaggggcgc ccgagtcggc cggccggccg gcgagaatct gcgcatgacc gatccctgga
     2041 cgatccggcc cgtcgcccct gacgacgcgg cacgcgtggc ggcgcaccga ggcttcgcgc
     2101 cgacggacgc agagggcccg gccggctacg ccgcctggct gcgtccgcgc atcgcgtcgg
     2161 gcgcctacgt cggccggctc gcgatcgccg cggacggcgc ggtcgtcggc ggcgcgggtg
     2221 cggtgctgct cgattggggg ccgacgcgcg gcaatccggg cggcacgatg gcgcgcatcg
     2281 tcaacgtctt caccgacgag ccctggcggc gccgcggcat cgcgcgggcg ctggtgcagg
     2341 cggtgctcgc ccggtgcgag gaagcgggcg tgcgtgagtt caacctcggc gcgacgccag
     2401 aggccgccgc gctctacgcc tcggtcggct tcggcccgta ttcggcggag atgcggcgcc
     2461 gcgtccccag tgcgtgatgt cgatggcgtg atgtcgatgt cgcgatcacg gaatcgacgc
     2521 cccgatcagc gtcggtcgtc tcctgcgggt tccggctgac ccgccgtggt ggtcagccct
     2581 cgcgcgcctt cagccgcacg ggcgtgatct cgacggtgtt cttcgtgtcg gagacccaca
     2641 ccgtgccgtc ctgcacggtg acctgccagg ccatcgtgcg ctcgacgagc gccgcgagcg
     2701 cctgcgagga ctcgggcgcg atcgaccaga cctcgacctt gggcgcgcgc acgagcttgc
     2761 cctgcaggcc ctgccaccag atcggcgtgc tcgaggcgta gctgtagacg gtgacccgct
     2821 cggcgcggct ggcggccttg gacaggcgcc gctcgtcggg ctggccgagc tcgatccact
     2881 gcaccacctg gccggtcagg tcgacctgcc agaggtcggg ctcgtccgtg tcccagaggc
     2941 tcttggcgag ctcgagccgg ccgcgctcgt cgtccgccgg cacgttgagc gcgtaggcga
     3001 gcacgcgcac cagcatgcgt tcgtcggtct ccgaggggtg gcgggcgatc gtcagatcgt
     3061 ggtcgccgta gaggtttcgg tccatgtcgg cgagttgcag ccggaccttg tggatcgtcg
     3121 ctttgagggc catcccgcca ttgtcgccgg gcggcgggcc gcgggcggcg cgacctcacc
     3181 aggcggcgtc gccgcacagg atcagcggct cgccggcgcg ctcgaactgg atcgacaccg
     3241 tcacgcacat gcgccggccc tgcagcgtca tgtacggccg cgtcacctgc gcgatgttgg
     3301 gctggcgctg ggcgctgcgg aagtaggcgc ggcgcgacca tcgcgcctcg cgcgtgtcgt
     3361 gcagcggcgc gaaccgggcg agtccctcga gctgctgctg cggcgagccc gcgccgatgg
     3421 cgttggtgac gacctggcgg ccgcggcgat cgagcaggta gcacatgtcc gcgcccggca
     3481 gcgcgaggaa gcgccggcag gcctcctcca gcgtcgcgcc gccggccagc agcacgctgg
     3541 cgagctcgat cgcctgccgg tacggcgtca tgcgcgcctg ccaggcctgg tcgtcggtgg
     3601 aggactggcg gtcgaagcga tcccacgccg cctgcagcgc atgcaggccg gtgcggccgg
     3661 cggcgcggcc cggcgcgggc aggccgaagt gccagccctg cacgaagtcg acgtcgcagc
     3721 gcagcgcgat gtcggcgccc tcggcggtct cgatgccctc catcagcacg aggcagccgc
     3781 actcgtgcag cagcgagacc atctgcgccg cgatgcgcag ggcgtgggcg tcgccctcgg
     3841 cgcgcacgac gacgctgcgg tcgagcttga cgatctcggg acgcagccgg aagacgcggt
     3901 cgaagttgga atggccggcg ccgaagtcgt ccagcgagac caggcagccg agcccgcgca
     3961 gcgcgtcgag ccggcggtcg aacgcggcgt cgtcgggcat ggaggactcg agcacctcga
     4021 tgacgacctg ctccggccgc aggccgtgcg cggcgaaggc ggcgccgatc tcgccgaggc
     4081 gatcggccag gggcgagccg agcgtgcgcg gatgcacgtt caggaacagc cactccggat
     4141 gcggcccggc ggcatgcgcg cgcaggtgct gcagcacggc ggcgcggtcg aggtcgacgc
     4201 gggcggcatc gtcggcgcag gcgtcgaaca gcgcctgcgg cgcgaccgcc atgccggtgg
     4261 cgaggtcgtc ggcgcgcaag agcgcctcgt ggccgaccac ccgctggtgc gtgaggctgt
     4321 agatcggctg gaagtgagac cgcaggcgca cgccgcggtg ctcggagctc g
//
LOCUS       DASVLN010000027         6783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_162654, whole genome shotgun sequence.
ACCESSION   DASVLN010000027 DASVLN010000000
VERSION     DASVLN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162654"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VF453_00745"
     CDS             <1..1143
                     /locus_tag="VF453_00745"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEX7686194.1"
                     /translation="RLGFYKTATAPGRLWIHAVSLGETRAASALIDALRERDPGIRIL
                     LTQSTATGREAALSLLRDGDALAWLPYDTPGAVRRFLARTQPAVGILMETEIWPQLLH
                     AAQARGLKMVLANARLSEKSARKGDRLAALMRPAAAAVTEVLAQTADDAERLREAGAP
                     SVALCGNLKFDVQPPQALVARGRQWREALGRPVVLAAITRDGEEAELLREWGRATGRS
                     PFLIVVPRHPQRFDEVEALMRGNGLRMARRSAWNEMPDAAQLQVDAWLGDSLGEMPLY
                     YGAADVALLGGSFMPFGGHNLIEAAACACPIVIGPSTYNFAEAAKQAVASGAAWQEQD
                     MGAAVRRALQVVSTELQALGSSRALAFAAQHRGAAQRMADHIARLL"
     gene            complement(1165..2547)
                     /locus_tag="VF453_00750"
     CDS             complement(1165..2547)
                     /locus_tag="VF453_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX7686195.1"
                     /translation="MKTIARGTPLACALALALGAGAAQAQSLTEVVDAARGYDAAYLG
                     ARANADAVRYRYEQQLALHRPSVGAQLQGQYNDQHAPYLAAQGGATAAKSDSLAASLQ
                     ASQTLFNRQNDYTIDAADKQVAVAESQVAQAEQDLIIRVAQAYFDVLAAADALQTVQA
                     NGKAISEQLASAKRNFEVGNATITDTREAEARFDLAHSQEISAQNDLQVARVKLDQLV
                     GRNGIAPHPLALPAALPPVMPSQASDWVSLAESDNPQLKQAQLARDIADLDIASAKAG
                     YLPTVSLTAGYNKTHQRDRFTTGVIGQNGAVESFNGSDSGWGNGANVTLTVRVPLYTG
                     GQLQNRVRETVALQEKASSDLEGARNAATLATRQAFLSAQSLNAQVKALEAAENSSQL
                     SLDATNLGYKVGVKVNLDVLNAQSQLYSTQKDLAAARYNYLMSTLKLRQAAGTLTTND
                     LLAVNALLVK"
     gene            complement(2807..3457)
                     /locus_tag="VF453_00755"
     CDS             complement(2807..3457)
                     /locus_tag="VF453_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX7686196.1"
                     /translation="MNIEQARFNMIEQQIRPWNVLDKGVLGLLAVVRREDFVPAAYRA
                     LAFMDTEVPLPDGESMLPPRVEARLLQDAQVQRHERVLEIGTGSGFMAALLGHRAQSV
                     ITLEIKPALAKLATENLRRSGAANVTVVQADGAHGYASEAPFDVIMLSGSVAKVPQEL
                     LAQLKPGGRLLAIEGQEPVMTAVRYTHSGSGFERTELFDIDAPRLHGFPEPERFHF"
     gene            complement(3520..4200)
                     /locus_tag="VF453_00760"
     CDS             complement(3520..4200)
                     /locus_tag="VF453_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7686197.1"
                     /translation="MSPFPPEHCPFRRRRKEARPAELLEAALALFVEKGFAATKSEEV
                     ARAAGVSKGTLYLYFPSKEELLKAVIQHFLANEIAAGAEEAAALEGPTPEMMEQLLVS
                     WWTRIYEGPASGVFKLVVTEVRNFPEIAQFWNERVVEPGNGLVRQLLERGVARGEFSP
                     LPHLDAAVHSILMPMILLCIHKHSLGACGVAEDLAKDAVGFLREHLRIVFKGLEVREA
                     PAAARGAA"
     gene            4371..4967
                     /locus_tag="VF453_00765"
     CDS             4371..4967
                     /locus_tag="VF453_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686198.1"
                     /translation="MDGQQGDRRAFGRAMAAMAAVALAGAVLGLGGCSTLAGSHDIVL
                     SESHLTLLLAREFPMERKVLEVIDLSVANPQIHLLPETNRLGTELDVTATDRIFGNTA
                     QAHVRLDYGLRFEPGDHSIRMINVRVRELTLASGSNQLHGVAQRLGTLVAEDVLENRA
                     LYTMKPAQADEMDRLDLVASPIVVTAQGVTVHVAPRGR"
     gene            5079..5918
                     /locus_tag="VF453_00770"
     CDS             5079..5918
                     /locus_tag="VF453_00770"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7686199.1"
                     /translation="MDIVLLIKAAVMGVVEGLTEFLPVSSTGHLILTSSLLGLANDEI
                     MKTFEVVIQSGAILAIVIVYWQLIVQSFANFGSDPKARRLIVNIVVGFLPAAVIGFLM
                     IKVIKQYLFNGPVVGGAFIVGALIILWVERGSATRRVRVHSIDEMTTADALKVGFIQC
                     LGMIPGTSRSGSTIIGGMLVGLSRQVATEFSFFLAIPTLVGASVLSAWKVRHELAAQW
                     HEYLPLFAVGFVVSFLAAWVCVRWLLRYVATHTFVAFAWYRIAFGLVVLATWVTGMVH
                     WAD"
     gene            complement(5951..6715)
                     /gene="trmB"
                     /locus_tag="VF453_00775"
     CDS             complement(5951..6715)
                     /gene="trmB"
                     /locus_tag="VF453_00775"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX7686200.1"
                     /translation="MTPDPRDDAPRDAAPSPSSTEAASASPDAAPAHPRRAIRSFVLR
                     AGRMGTGQQKAMETLAPRYVLPHAARPIDFAAVFGRTAPVILEIGFGMGDATARIAAA
                     RPDDDFIGVEVHAPGVGALLKRIGEQGLSNLRIVQHDAVEVLRDMIPPGSLAGIHVYF
                     PDPWHKKKHNKRRLIQPAFAAELAARLGTGGYLHCATDWQPYAEQMLEVLSAEPTLAN
                     TAAGYAPRPEWRPLTKFENRGLQLGHGVWDLVFTKR"
BASE COUNT          884 a         2351 c         2499 g         1049 t
ORIGIN      
        1 cgtctcggct tctataagac cgcgacggcg cccggccgcc tctggatcca cgccgtgtca
       61 ctcggcgaga cccgggccgc cagcgcgctg atcgacgccc tgcgcgagcg cgatccgggc
      121 atccgcatcc tgctgaccca gtccaccgcc accggccgcg aggccgccct ctccctgctg
      181 cgcgacggcg acgccctggc ctggctgccc tacgacacgc ccggcgccgt gcgccgcttc
      241 ctcgcgcgca cgcagccggc ggtcggcatc ctgatggaga ccgagatctg gccacagctg
      301 ctgcacgcgg cgcaggcacg cgggctcaag atggtgctgg ccaacgcgcg actgtccgag
      361 aagagtgcga ggaagggcga tcgcctggcc gcgctgatgc gcccggccgc ggccgccgtc
      421 accgaggtgc tcgcgcagac cgccgacgac gccgagcgcc tgcgcgaggc cggcgcgccg
      481 agcgtcgcgc tgtgcggcaa tctcaagttc gatgtccagc cgccgcaggc gctcgtcgcg
      541 cgcggccgcc agtggcgcga ggccctcggc cggccggtgg tgctggccgc gatcacgcgc
      601 gacggcgagg aggccgaact gctgcgcgaa tggggccggg cgaccggccg cagccccttc
      661 ctcatcgtcg tgccgcgcca tccgcagcgc ttcgacgagg tcgaggccct gatgcggggc
      721 aacggcctgc gcatggcccg ccgcagcgcc tggaacgaga tgcccgacgc ggcgcagctg
      781 caggtcgacg cctggctggg cgactcgctc ggcgagatgc cgctgtacta cggcgccgcc
      841 gacgtcgcgc tgctgggcgg cagcttcatg cccttcggcg gccacaacct catcgaggcc
      901 gcggcctgcg cctgcccgat cgtgatcggg ccgtcgacct acaacttcgc cgaggccgcg
      961 aagcaggccg tcgcctcggg cgccgcctgg caggagcagg acatgggcgc cgcggtgcgg
     1021 cgcgcgctcc aggtcgtcag caccgagctg caggccctcg gcagctcccg cgcgctcgcg
     1081 ttcgccgcgc agcaccgggg cgccgcgcag cgcatggcgg accacatcgc caggctgctg
     1141 tgagcgcggc gcggccgcgc gcgatcactt cacgagcagc gcgttgaccg ccagcaggtc
     1201 gttggtcgtg agcgtgcccg ccgcctggcg cagcttgagc gtcgacatga ggtagttgta
     1261 gcgcgcggcc gccagatcct tctgggtcga gtacagctgc gactgcgcgt tcagcacgtc
     1321 gaggttgacc ttcacgccga ccttgtagcc gaggttggtg gcgtccagcg aaagctggct
     1381 cgagttctcg gcggcctcca gcgccttgac ctgcgcgttc agcgactgcg cgctgaggaa
     1441 ggcctggcgc gtggcgagcg tggcggcgtt gcgcgcgccc tcgaggtcgc tgctggcctt
     1501 ttcctgcagc gcgacggtct cgcgcacgcg gttctgcagc tggccgcccg tgtacagcgg
     1561 caccctcacg gtgagcgtga cgttggcgcc gttgccccag ccgctgtccg agccgttgaa
     1621 cgactccacg gcgccgttct ggccgatgac gccggtcgtg aaccgatccc gctggtgcgt
     1681 cttgttgtag ccggcggtca gcgatacggt gggcaggtat ccggccttgg cgctggcgat
     1741 gtcgaggtcg gcgatgtcgc gcgcgagctg cgcctgcttc agctgcgggt tgtcgctctc
     1801 ggccagcgaa acccagtcgg aggcctggct cggcatgacg ggaggcagcg ccgccggcag
     1861 cgcgagcggg tgcggcgcga tgccgttgcg gccgacgagt tggtcgagct tcacccgcgc
     1921 gacctgcagg tcgttctgcg cggagatctc ctgcgagtgc gcgaggtcga agcgcgcctc
     1981 ggcctcgcgg gtgtcggtga tggtggcgtt gccgacctcg aagttgcgct tggccgaggc
     2041 cagctgctcg ctgatcgcct tgccgttggc ctgcacggtc tgcagcgcgt cggcggcggc
     2101 cagcacgtcg aagtaggcct gggccacgcg gatgatgaga tcctgctccg cctgcgccac
     2161 ctgcgactcg gcgaccgcga cctgcttgtc ggccgcgtcg atcgtgtagt cgttctggcg
     2221 gttgaacagc gtctgcgacg cctgcaggct ggccgccagc gagtccgatt tcgcggccgt
     2281 cgcgccgccc tgcgccgcga ggtagggcgc gtgctggtcg ttgtactggc cctgcagctg
     2341 cgcgccgacg ctcgggcggt gcagcgcaag ctgctgctcg tagcggtagc gcacggcgtc
     2401 cgcgttggcg cgcgcgccga ggtaggccgc gtcgtagccg cgcgccgcgt ccaccacctc
     2461 ggtcaggctc tgggcctgcg ccgcgccggc gccgagcgcc agggccagcg cgcacgcgag
     2521 cggcgtgccg cgggcgatcg ttttcatggg acctgggcgc gggtgcgaca tgggcaatcc
     2581 ttctggttgg caggttctcg ccgggaccgg gcggtcggcg tcgggacagg tttgtacggg
     2641 ctcgcaccgg acgatgcacg cccgatgcga cgatctgcag acagcaaggc ccctgtcgac
     2701 gatttgagcc gacagactgc gggcgattgg cgatgacggc ggacgccgcg cgagcgtccg
     2761 caggggcttc cggcgcgcgt ctttcgaacg gcgcgcgccc ggggactcag aagtggaagc
     2821 gctcgggttc ggggaatccg tgcaggcgcg gggcgtcgat gtcgaacagc tcggtgcgct
     2881 cgaagccgct gccggaatgg gtgtagcgca ccgcggtcat cacgggctcc tggccctcga
     2941 tggcgagcag gcggccgccc ggcttgagct gggcgagcag ctcctgcggc accttggcga
     3001 cggagcccga gagcatgatc acgtcgaatg gcgcctcgga ggcgtagccg tgcgcgccat
     3061 cggcctgcac caccgtcacg ttggcggcgc cgctgcggcg caggttctcg gtggcgagct
     3121 tggccagcgc cggcttgatc tccaacgtga tcacgctttg cgcgcggtgt cccagcaggg
     3181 cggccatgaa gcccgagccg gtgccgatct cgagcacgcg ctcgtggcgc tgtacctggg
     3241 cgtcctgcag caggcgggcc tcgacgcgcg ggggcagcat cgattcgccg tccggcagcg
     3301 gcacctcggt gtccatgaag gcgagcgcgc ggtaggcggc cggcacgaag tcctcgcggc
     3361 gcacgacggc cagcaggccc agcacgccct tgtccagcac gttccacggc cggatctgct
     3421 gctcgatcat gttgaagcgg gcttgttcga tgttcatggt gtcctcggtc ggaaagggag
     3481 ctgtgcgggc cgcgcgcgct gcgggcaaac ccgcaaattc tatgcggccc cgcgggccgc
     3541 ggcgggggcc tcgcgcacct cgaggccctt gaagacgatg cgcaggtgct cgcgcaggaa
     3601 gccgacggcg tccttggcca ggtcctcggc gaccccgcag gcgcccagcg aatgcttgtg
     3661 gatgcacagc aggatcatcg gcatgaggat ggagtgcacg gccgcgtcga ggtggggcag
     3721 cggcgagaac tcgccgcgcg cgacgccgcg ctcgagcagc tggcgcacca gcccgttgcc
     3781 cggctcgacc acgcgctcgt tccagaactg cgcgatctcg gggaagttgc gcacctccgt
     3841 cacgaccagc ttgaagacgc ccgacgccgg gccttcgtag atgcgcgtcc accacgagac
     3901 caggagctgc tccatcatct ccggggtcgg gccttccagg gccgcggcct cctcggcgcc
     3961 ggcggcgatc tcgttggcca ggaagtgctg gatgacggcc ttcagcagct cctccttgct
     4021 cgggaagtag aggtacagcg tgcccttgga gacgcccgcc gcgcgcgcca cctcctcgct
     4081 cttggtggcc gcgaagccct tctcgacgaa cagggcgagc gcggcctcca gcagctccgc
     4141 cggccgcgct tccttgcggc ggcggcggaa cgggcagtgc tcgggcggga acgggctcat
     4201 cgcgggatcc gggtcggggt tgccggaatt actgactcat tggtcagtaa tcttatcagc
     4261 atttgcggcg cttgtgtcgg cgaagcggcg tcgcggcgcg ttgaccaggg aacacggact
     4321 gccggcgggt gcggcggatg cgacgaccac gacggcgaga ggaacagggc atggacgggc
     4381 agcaaggcga tcgacgggcg ttcggcaggg cgatggcggc catggcggcg gtcgcgctcg
     4441 cgggcgcggt gctgggcctc ggcggctgct cgacgctggc cggctcgcac gacatcgtgc
     4501 tgagcgagtc gcatctcacg ctgctgctgg cgcgcgagtt cccgatggag cgcaaggtgc
     4561 tcgaggtgat cgacctgagc gtcgccaacc cgcagatcca cctgctgccc gagacgaatc
     4621 gcctcggcac ggagctcgac gtcaccgcga cagaccgcat cttcggcaac accgcccagg
     4681 cgcacgtgcg gctcgactac ggcctgcgct tcgagcccgg cgaccattcg atccgcatga
     4741 tcaacgtgcg ggtgcgcgag ctgacgctgg cgagcggctc caaccagctg cacggcgtgg
     4801 cgcagcgcct cggcacgctg gtcgccgagg acgtgctcga gaaccgcgcg ctctacacga
     4861 tgaagccggc gcaggccgac gagatggatc gcctcgacct cgtcgcctcg cccatcgtcg
     4921 tgacggccca gggcgtgacg gtgcacgtcg cgccgcgcgg tcgctgacgc ccggatcgcg
     4981 cgcgcccggc tgcaggacag gcagcggtca agtcgcccgc gtatcatcgc gcgcttgtcc
     5041 aaaccagggc ccagccggcc cgctccggag tgcagcgcgt ggatatcgtc ttgctgatca
     5101 aggccgccgt catgggcgtc gtcgagggcc tcaccgagtt cctccccgtg tcgtcgacgg
     5161 gtcacctgat cctcacgagc tcgctgctcg gcctggccaa cgacgagatc atgaagacct
     5221 tcgaggtcgt gatccagtcc ggcgcgatcc tggcgatcgt gatcgtctac tggcagctca
     5281 tcgtccagtc cttcgccaac ttcggcagcg acccgaaggc gcgccggctg atcgtcaaca
     5341 tcgtcgtcgg cttcctgccc gccgcggtga tcggcttcct gatgatcaag gtcatcaagc
     5401 agtacctgtt caacggcccc gtcgtgggcg gcgcgttcat cgtcggcgcg ctgatcatcc
     5461 tgtgggtcga gcgcggcagc gcgacgcgcc gcgtgcgcgt gcactcgatc gacgagatga
     5521 ccaccgccga cgcgctcaag gtcggcttca tccagtgcct gggcatgatc ccgggcacca
     5581 gccgctcggg ctcgacgatc atcggcggca tgctggtggg gctgtcgcgc caggtggcga
     5641 ccgagttcag cttcttcctc gcgatcccga cgctggtcgg cgcgagcgtg ctgagcgcgt
     5701 ggaaggtgcg ccatgagctg gccgcccagt ggcacgagta cctgccgctg ttcgcggtcg
     5761 gcttcgtcgt gtccttcctc gcggcctggg tctgcgtgcg gtggctgctg cgctacgtcg
     5821 ccacgcacac cttcgtcgcc ttcgcgtggt accggatcgc gttcggcctc gtggtgctcg
     5881 cgacctgggt caccggcatg gtgcactggg ccgactgagg cgttctcggc ccgcgggccg
     5941 cgcgccgcgg tcagcgcttc gtgaagacca ggtcccagac gccgtgcccc agctggaggc
     6001 cgcggttctc gaacttggtc agcggccgcc actcgggccg cggcgcgtag cccgcggccg
     6061 tgttggccag ggtgggttcc gcggacagca cctccagcat ctgctcggca tagggctgcc
     6121 agtcggtcgc gcaatgcagg tagccgccgg tcccgaggcg ggcggcgagc tcggcggcga
     6181 acgccggctg gatcaggcgc cgcttgttgt gcttcttctt gtgccacggg tcggggaagt
     6241 acacgtggat gcccgccagc gagccgggcg ggatcatgtc gcgcagcacc tcgacggcgt
     6301 cgtgctggac gatgcgcagg ttggacaggc cctgctcgcc gatgcgcttg agcagcgcgc
     6361 cgacgccggg tgcgtgcacc tcgacgccga tgaagtcgtc gtccggccgc gccgcggcga
     6421 tgcgcgcggt ggcgtcgccc atcccgaagc cgatctccag gatcaccggc gccgtccggc
     6481 cgaagaccgc cgcgaagtcg atcggccgtg cggcgtgcgg gagcacgtag cgtggggcga
     6541 gcgtctccat cgccttctgc tggccggtgc ccatgcggcc ggcgcgcagc acgaagctgc
     6601 ggatcgcgcg acgcgggtgc gccggcgcgg cgtcgggcga ggcgctggcg gcctcggtgg
     6661 aggaagggga cggggcggcg tcccgcggcg cgtcgtcgcg gggatcagga gtcatcgtgc
     6721 ggtcgggttc tcgggcaggg ggcgcggcgg cgtcgcgtcg ccgcgcggcg aactggggcg
     6781 gcg
//
LOCUS       DASVLN010000028         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_431471, whole genome shotgun sequence.
ACCESSION   DASVLN010000028 DASVLN010000000
VERSION     DASVLN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431471"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF453_00780"
     CDS             <1..199
                     /locus_tag="VF453_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686201.1"
                     /translation="GSLAAWRDSVADDSPLRRRYQTALLQQATQVLTSCLHVQALAAS
                     ESERCAVSRQDIAVRADPASP"
     gene            266..1744
                     /locus_tag="VF453_00785"
     CDS             266..1744
                     /locus_tag="VF453_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7686202.1"
                     /translation="MAERHEQRSLFGEILDWMLAPLLLLWPMSVALTWLVAQGIANRP
                     YDRELDQLAHSVARQMRADPAFDRGLVGRRDIRLDESTAALLRSDDVDRVYFQVRDAR
                     GGLAGGDGELAAPADDHAEPDEVQFRDDELHHDPVRVAWLWVGTTTGVNSGVVVQVAE
                     TFDRRSRLATEIIKGVILPQFVILPLAVLLVWFALARGIAPLNALQQRIRRRESQDLG
                     PIDDHDVPEEVAPLVRSINDLLARLDRTMGTQKHFLADAAHQMKTPLAGLRMQAELAQ
                     REIDAGGDPQAIKGSLQQIARSSQSAAHLVNQLLAMARAEDTEQARRRQDVPIVKLAT
                     EVVRDFVPRAMDKRIDLGYEGPEAEPATPRLRGQPVLVRELIRNLVDNALQYTPAGGT
                     VTVRLMPDPYGRVVVLQVEDTGPGIPEAEREQVFQPFYRALGTNVDGSGLGLAIVKEI
                     ADKHGASVTIEPARTPPHGSGESPGALFTVRFPCEAEAPPAA"
     gene            complement(1749..2903)
                     /gene="rodA"
                     /locus_tag="VF453_00790"
     CDS             complement(1749..2903)
                     /gene="rodA"
                     /locus_tag="VF453_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX7686203.1"
                     /translation="MIAVVERPTVWQRLRPILTGFDPILMIALILICGIGLVTMYSAG
                     YDYGTRFSEHARNMALAFGVLFVAAQVSPQNLMRLAVPVYTVGLALLVATALFGVTKK
                     GAQRWLNVGMVIQPSEIMKLAMPLMLAWWFQKREGKLTAVDFAIAGLILMVPVALVVK
                     QPDLGTAILILSAGLYVIFFAGLSWKLILPVLALAAIGIAAVVWSEDRICQDDVVWPL
                     LRDYQKHRICTLLDPTTDPLGKGFHIIQGMIAIGSGGVTGKGFMSGTQTHLEFIPERT
                     TDFIFAAFSEEFGMLGALLLLFGFTLLILRGLAIAADAPSVFTRLLAGSITLSFFTYA
                     FVNMGMVSGILPVVGVPLPFVSYGGTAMVTLGLGLGFLTSIARNRRSVQT"
BASE COUNT          473 a         1086 c         1070 g          377 t
ORIGIN      
        1 ggggtcgctc gccgcgtggc gagacagcgt cgccgacgac agcccgctgc gccgccgcta
       61 ccagaccgcg ctgctgcaac aggcgaccca ggtgctcacg tcctgcctgc acgtgcaggc
      121 gctcgcggcc agcgagtcgg agcgctgcgc ggtctcgcgc caggacatcg ccgtccgcgc
      181 cgacccggca tcgccctgac gcgcgccgcc cacgcgggag ccgcccgccg cgcccgccgg
      241 tgcgtcacgc tatgctgggc gccccatggc cgaacgccac gaacaacgct ccctcttcgg
      301 cgagatcctc gactggatgc tggcgcccct gctgctgctg tggccgatga gcgtcgcgct
      361 gacctggctc gtcgcccagg gcatcgccaa ccggccgtac gatcgcgagc tcgaccagct
      421 cgcccacagc gtcgcccgcc agatgcgcgc cgaccccgcc ttcgaccgcg gcctcgtcgg
      481 gcggcgcgac atccgcctcg acgagtcgac ggccgccctg ctgcgcagcg acgacgtcga
      541 ccgggtctac ttccaggtgc gcgacgcgcg cggcgggctc gcgggcgggg acggcgaact
      601 cgcggcgccc gccgacgacc atgcggagcc cgacgaggtg cagttccgcg acgacgagct
      661 ccaccacgat ccggtgcgcg tggcctggct gtgggtgggc acgaccaccg gcgtcaacag
      721 cggcgtcgtc gtgcaggtgg ccgagacctt cgaccgccgc tcgcgcctgg ccaccgagat
      781 catcaagggc gtgatcctgc cgcagttcgt catcctgccg ctggcggtgc tgctggtgtg
      841 gttcgcgctc gcgcgcggca tcgcgccgct caacgcgctg cagcagcgca tccgccggcg
      901 cgagagccag gacctcggcc cgatcgacga ccacgacgtg cccgaggagg tcgcgccgct
      961 ggtgcgctcg atcaacgacc tgctcgcgcg cctggatcgc acgatgggca cgcagaagca
     1021 cttcctggcc gacgccgcgc accagatgaa gacgccgctg gcggggctgc ggatgcaggc
     1081 ggagctcgcg cagcgcgaga tcgacgccgg cggcgacccg caggcgatca agggctcgct
     1141 gcagcagatc gcgcgctcga gccagagcgc ggcgcacctg gtcaaccagc tgctcgcgat
     1201 ggcgcgcgcc gaggataccg agcaggcgcg ccggcgccag gacgtcccga tcgtcaagct
     1261 cgccacggag gtcgtgcgcg acttcgtgcc gcgcgcgatg gacaagcgca tcgacctcgg
     1321 ctacgaaggg ccggaagccg agcccgccac tccgaggctg cgcggccagc cggtgctggt
     1381 gcgcgagttg atccgcaacc tcgtcgacaa cgcgctgcag tacacgcccg ccggcggcac
     1441 ggtgaccgtg cgcctgatgc ccgacccgta cggccgcgtc gtcgtgctgc aggtcgagga
     1501 cacgggcccc ggcatccccg aagccgaacg cgagcaggtg ttccagccgt tctaccgcgc
     1561 gctcggcacc aacgtggacg gcagcggcct ggggctggca atcgtcaagg agatcgcgga
     1621 caagcacggc gcgagcgtca cgatcgagcc cgcgcgcacg ccgccccacg gctcgggcga
     1681 gtcgcccggc gcgctcttca cggtgcggtt cccgtgcgag gcggaggccc cgcccgcggc
     1741 gtgagatgtc aggtctgcac cgaccgccgg ttgcgcgcga tcgacgtcag gaacccgagc
     1801 ccgagcccga gcgtcaccat cgccgtgccg ccgtagctca cgaacggcag cggcacgccg
     1861 acgacgggca ggatgccgct gaccatgccc atgttgacga acgcgtacgt gaagaagctg
     1921 agcgtgatcg agccggcaag caggcgcgtg aagacggacg gggcgtcggc ggcgatcgcc
     1981 aggccgcgca ggatcagcag cgtgaagccg aacagcagca acagcgcgcc gagcatgccg
     2041 aactcctcgg agaacgcggc gaagatgaag tccgtggtgc gctcggggat gaattcgagg
     2101 tgcgtctgcg tgccgctcat gaagcccttg cccgtcacgc cgccggagcc gatcgcgatc
     2161 atgccctgga tgatgtggaa gcccttgccg agcgggtcgg tggtcgggtc gagcagcgtg
     2221 cagatgcggt gcttctggta gtcgcgcagc agcggccaga cgacgtcgtc ctggcagatg
     2281 cggtcctcgc tccagacgac ggccgcgatg ccgatcgccg ccagcgcgag caccggcagg
     2341 atcagcttcc aggacagccc ggcgaagaag atcacgtaga ggcccgccga caggatcagg
     2401 atggcggtgc cgaggtcggg ctgcttgacg accagcgcca ccggcaccat caggatcagc
     2461 ccggcgatcg cgaagtccac cgcggtgagc ttgccctcgc gcttctggaa ccaccaggcg
     2521 agcatcagcg gcatcgcgag cttcatgatc tcgctgggct ggatcaccat gccgacgttg
     2581 agccagcgct gcgcgccctt cttcgtgacg ccgaacagcg ccgtcgcgac gagcagcgcg
     2641 aggcccaccg tgtagaccgg caccgccagg cgcatcaggt tctgcggcga gacctgcgcc
     2701 gcgacgaaca gcaccccgaa cgccagcgcc atgttgcgcg catgctccga gaagcgcgtg
     2761 ccgtagtcgt agccggccga gtacatcgtg accaggccga tgccgcagat caggatgagc
     2821 gcgatcatca ggatcgggtc gaagccggtg aggatcggcc gcaggcgctg ccagacggtg
     2881 ggacgttcga cgacggcgat catcgcggct ccttcgggtt ggcggccggc gtccaggacg
     2941 cggccgggcg gcgcgtgggg gccgacgcgc ccgacgcgcg ctgggcgacc gcgccctcgc
     3001 cggcat
//
LOCUS       DASVLN010000029         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_324041, whole genome shotgun sequence.
ACCESSION   DASVLN010000029 DASVLN010000000
VERSION     DASVLN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324041"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VF453_00795"
     CDS             complement(<1..533)
                     /locus_tag="VF453_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686204.1"
                     /translation="MLSKNSRRELVLMLVVTLLFWAAARLGLVFNDNGYLTPVWPPAA
                     VACVAGILYGPRCLIGAALYIAYDFMDGDFGSLSHDRWALVEPLAMLASATVCREAAR
                     RSHFDGRLDTLRAVMRMMGIGVLFAFVNGAGATLGYCGLAGTQRCLAYGWSGYWVQSM
                     IGDVFGCLICMPALLSWA"
     gene            783..1505
                     /gene="serB"
                     /locus_tag="VF453_00800"
     CDS             783..1505
                     /gene="serB"
                     /locus_tag="VF453_00800"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX7686205.1"
                     /translation="MTSGSDPSALPAGLVVRGFAPPLRLADFRLAAFDMDSTLIDIEC
                     IDEIAAAVDLKAEVAAITEATMRGEIADFKESLRRRVALLRGVPESALQEIHDRRLHL
                     NPGVEAFMAALRAAGVKTLLVSGGFTFFAERVAARLGMDYMRSNVLEVVEGRLTGRVV
                     AQHWGDIVDGEEKRRTMLQVCAELGCAPRQAIAVGDGSNDLPMMEAAGLSIAYHAKPV
                     VRAKAMLSIEEGGMDRALAVLR"
     gene            complement(1525..1947)
                     /locus_tag="VF453_00805"
     CDS             complement(1525..1947)
                     /locus_tag="VF453_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686206.1"
                     /translation="MNTGLVTPDRRYLVVNGRLWRAANPTLPAADRVRFTRELMAARR
                     AVRAAKDAGDELALRRARIAVNAAKQGLGERGAVWWSDGAPDYNRTLVKNTPYAQWYA
                     GVMAERADADAAARPDSPPPGFIASHFGGLSSLGGRAA"
     gene            complement(2039..>2848)
                     /locus_tag="VF453_00810"
     CDS             complement(2039..>2848)
                     /locus_tag="VF453_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEX7686207.1"
                     /translation="ELMEIAKGGQTLIGFPALIDKGTHVEIEVFDEPDVAAAKHRAGL
                     RRLLALALKEPIRFLERNLPDLTRMSVLYMPLGTAEELRDQVIGVAIDRAFLDEPLPA
                     SEDDFKRRLEAGRSRLNLIAQEVARQAHLVLTEYATAARKLKDARPPKDVADDVQQQL
                     QRLVGKRFVQETPWAQLQHLPRYLKGVTLRLDKLRADPARDAQRLAELRPVEQRFLRA
                     LAERKGAADPRLAEYRWLLEELRVSLFAQELRTPQPVSVKRLDKAWEQLTR"
BASE COUNT          399 a         1015 c         1050 g          384 t
ORIGIN      
        1 gcccaggaca gcagcgccgg catgcagatc aggcagccga agacgtcgcc gatcatcgac
       61 tgcacccagt agcccgacca gccgtaggcg aggcagcgct gggtgcccgc gaggccgcag
      121 tagccgagcg tcgcgcccgc gccgttgacg aaggcgaaca gcacgccgat ccccatcatg
      181 cgcatcacgg cgcgcagggt gtcgaggcgg ccgtcgaagt gcgagcgccg cgccgcctcg
      241 cggcacaccg tggccgaggc cagcatggcc agcggctcga cgagcgccca gcggtcgtgc
      301 gagaggctgc cgaagtcgcc gtccatgaag tcgtaggcga tgtacagcgc cgcgccgatc
      361 aggcagcgcg ggccgtacag gatgccggcg acgcaggcga ccgccgccgg cggccatacc
      421 ggcgtcaggt agccgttgtc gttgaagacc agccccagcc gggccgccgc ccagaacagc
      481 agggtgacga ccagcatcag gacgagttcg cgacgggagt tcttggacag caagctgggt
      541 cgttctctcg tacggggacg gtcgccgccc gcgccccgcg gcgtccgggc gacggccctc
      601 cggcgccgcg ccgggcgcgg ccccctcaaa acgtggattg tcatgcggaa tcgggccagg
      661 gaatgtgtcg atcggatccg cgtttccgcc ggaacgcgcc ggctgtcggg gcggcgcccg
      721 gcgggcctct tagaatcccg gcctcccttg cccgcttgcg ccgcgccggc gcccgctcgc
      781 ccatgacttc cggatccgat ccctcggccc tgcccgcggg cctcgtcgtg cgcggcttcg
      841 cgccgccgct gcgcctggcc gacttccgcc tcgccgcgtt cgacatggac tcgacgctga
      901 tcgacatcga atgcatcgac gagatcgccg ccgccgtcga cctcaaggcc gaggtcgcgg
      961 cgatcaccga agcgacgatg cgcggcgaga tcgccgactt caaggagagc ctgcgccgcc
     1021 gcgtcgcgct gctgcgcggg gtgcccgagt cggcgctgca ggagatccac gaccggcgcc
     1081 tgcacctgaa cccgggcgtc gaggccttca tggccgcgct gcgggcggcg ggcgtgaaga
     1141 cgctgctggt gtccggcggc ttcaccttct tcgcggagcg cgtcgcggcc cggctgggca
     1201 tggactacat gcgctccaac gtgctcgagg tggtcgaggg ccggctcacc ggccgcgtcg
     1261 tcgcgcagca ctggggcgac atcgtcgacg gcgaggagaa gcgccgcacg atgctgcagg
     1321 tctgcgcgga gctcggctgc gccccgcggc aggccatcgc ggtgggcgac ggctccaacg
     1381 acctgcccat gatggaggcg gccggactgt cgatcgcgta ccacgccaag cccgtggtgc
     1441 gcgcgaaggc gatgttgtcg atcgaggaag gcgggatgga ccgcgccctg gccgtgctgc
     1501 gctgaagcgc gccgcgaggc gcgctcaggc ggcgcgaccg cccagcgacg agagtccgcc
     1561 gaagtgcgag gcgatgaagc cgggcggcgg cgagtcgggg cgcgccgcgg cgtccgcgtc
     1621 cgcgcgctcg gccatcacgc ccgcatacca ctgggcgtac ggcgtgttct tgacgagcgt
     1681 gcggttgtag tcgggcgccc cgtcgctcca ccagacggcg ccgcgctcgc ccagtccctg
     1741 cttggcggcg ttgacggcga tgcgcgcgcg ccgcagcgcg agctcgtcgc cggcatcctt
     1801 ggcggcgcgc accgcgcggc gtgcggccat cagctcgcgg gtgaagcgca cgcggtcggc
     1861 cgcgggcagc gtcgggttcg cggcgcgcca caggcggccg ttgacgacga gatagcgacg
     1921 gtcgggcgtc acgagaccgg tgttcatgga cggcactgta ggcaaacggc gcgccggcca
     1981 tgtcacgaaa agcaaccgga aagacgcccc aactgtcgag cgggccgccc tgcgcccatc
     2041 agcgcgtcag ctgctcccag gccttgtcca ggcgcttgac gctcaccggc tgcggcgtgc
     2101 gcagctcctg cgcgaacagg ctgacgcgca gctcctcgag cagccagcgg tactccgcca
     2161 gccgggggtc ggcggcgccc ttgcgctcgg ccagggcacg caggaagcgc tgctccacgg
     2221 gccgcagctc ggccaggcgc tgcgcgtccc gggcgggatc ggcgcgcagc ttgtccagcc
     2281 gcagcgtcac gcccttgaga tagcgcggca ggtgctggag ctgcgcccac ggcgtctcct
     2341 gcacgaaacg tttgccgacg aggcgctgca attgctgctg gacatcgtcg gccacgtcct
     2401 tgggtggacg cgcgtccttg agcttgcgcg cggcggtcgc gtactcggtc aggacgaggt
     2461 gcgcctggcg cgccacctcc tgggcgatca ggttgagccg gctgcggccg gcctcgaggc
     2521 ggcgcttgaa gtcgtcctcg ctcgcgggca gcggctcgtc gaggaaggcg cggtcgatcg
     2581 cgacgccgat cacctggtcg cgcagctcct cggccgtgcc cagcggcatg tagagcacgg
     2641 acatgcgcgt gaggtcgggc aggttgcgct cgaggaagcg gatcggctcc ttgagcgcga
     2701 gcgcgagcag ccgccgcaag cccgcgcgat gcttcgcggc ggcgacatcg ggctcgtcga
     2761 agacctcgat ctcgacgtgc gtgcccttgt cgatcagcgc gggaaagccg atcagcgtct
     2821 ggccgccctt ggcgatctcc atcagctc
//
LOCUS       DASVLN010000030         3607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_485062, whole genome shotgun sequence.
ACCESSION   DASVLN010000030 DASVLN010000000
VERSION     DASVLN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485062"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VF453_00815"
     CDS             <1..1063
                     /locus_tag="VF453_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7686208.1"
                     /translation="SLFSAGAGVDWTPDVFGGGRRRVEQARALVDYQRAQRRAARLSL
                     VGNTVLQAIALASTREQLAAVEDILAADERNLALVELSAAAGKSAQLDVLTARSQLSS
                     DRALLPPLRQQASAARHALAVLAGERAGNWSPPEIGFDALAMPRELPLSLPSDWLRQR
                     PDIEAAEAQLRAANAQVGIATAALFPSFTLDGAWTATGRVPGRLFHAGTDVWSLAASL
                     LAPVFDGGTLAAQRAAAVDAYAAQLAAYRLVLLQAFGQVADVLDQSRNDAALLDVETQ
                     ALDAAAATLALTREGYRAGQASLVQLLDAQRLYEQARLGRARALGQRFSDCAQWFIAM
                     GEADDTGAAPVQATMRREP"
     gene            1197..1964
                     /locus_tag="VF453_00820"
     CDS             1197..1964
                     /locus_tag="VF453_00820"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX7686209.1"
                     /translation="MERLKDRVALVTGGAMGIGESTCRLMAREGAVVAVTDLDEVRGE
                     AVAASIRAAGGRAGFWHLDVAEESQVREVFSNVQSRLGAIRVLVNNAGIAGANKPTHE
                     LSVDEWERVMAVNVRGVFLCTKHVVPQMKSAGGGSIVNLSSIYGLVSAPDVPPYHASK
                     GAVRLMTKTDALLYAADRIRVNSIHPGFIRTPMVAGFMQTQADPAAAEAATAQLHPLG
                     HLGEPDDIGWACVYLASEESRFVTGSELVVDGGYTCR"
     gene            complement(1966..2691)
                     /locus_tag="VF453_00825"
     CDS             complement(1966..2691)
                     /locus_tag="VF453_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686210.1"
                     /translation="MRELRTPRRARVVAGTIAVAALAAASIGWMHTAGGRALLGRLGV
                     PCPVNTVDARQVLAVRDDAIMRTRGDALAPERPALGLKLDRSTEPQVAQRMASARAHC
                     DAIARGYRYLRCRGVAADALGVEGPPVSEIWFSFDPGGHLVAINLYRRGMDESQARQS
                     WRGAVDGLQRALGHPTTTTGDPSLVQLFSSPVAVARIQYAYADYVATVTASRLPYGGL
                     AVRELYMSAVTPRRLAPAPAPGA"
     gene            complement(2679..3365)
                     /locus_tag="VF453_00830"
     CDS             complement(2679..3365)
                     /locus_tag="VF453_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686211.1"
                     /translation="MKLATSFFAAIAAAALGGCGGDSDPPVATVQHPENAAIWTALGG
                     DASAPAAVASVVDAAVTGVLADPVEAPYFVAVTSNTSKSSAAHDTPARLKACLTLQFE
                     SLLGGPYSYPGPVTVAGDTDGQPEMCQDMTAAHSDVGVPGCVFDQFMVDLVGVLQAQL
                     PAADFAALTSTTNGTYALTASNAAGIAVGTKFQYSVVPTLFNLRGTITTATPTGPIYL
                     APTVATSCAS"
BASE COUNT          458 a         1304 c         1374 g          471 t
ORIGIN      
        1 gagcctgttc tccgccgggg ccggtgtcga ctggacgccc gacgtcttcg gcggcggccg
       61 ccggcgggtc gagcaggcgc gcgcgctcgt cgactaccag cgcgcgcagc ggcgtgcggc
      121 gcggctctcg ctcgtcggca acaccgtgct ccaggcgatc gcgctggcgt cgacccgcga
      181 gcagctcgcg gcggtcgagg acatcctcgc ggcggacgag cgcaacctcg cgctcgtcga
      241 gctgtcggcg gccgccggca agtcggcgca gctcgacgtg ctgacggcgc gcagccagct
      301 ctcgagcgac cgcgccttgc tgccgccgct gaggcagcag gcgagcgcgg cgcgccacgc
      361 gctggccgtc ctcgccggag agcgcgccgg caactggtcg ccgcccgaga tcggcttcga
      421 tgcgctggcc atgccgcgcg agctgccgct gtcgctgccc tcggactggc tgcgccagcg
      481 gcccgacatc gaggccgcgg aggcgcagct gcgcgccgcg aacgcgcagg tgggcatcgc
      541 gacggcggcg ttgttcccgt ccttcacgct ggacggcgcc tggacggcga cagggcgcgt
      601 gccgggccgg ctgttccacg cgggcacgga cgtctggagc ctcgccgcga gcctgctcgc
      661 gccggtcttc gacggtggga cgctcgcggc gcagcgggcc gcggcggtcg acgcctacgc
      721 ggcccagctc gcggcgtacc ggctcgtcct gctgcaggcg ttcggccagg tcgccgacgt
      781 gctggaccag tcgcgcaacg atgcggcgct gctggatgtc gagacccagg cgctcgacgc
      841 ggcggccgcg acgctcgcgt tgacgcgcga gggctaccgc gccggccagg cgagcctggt
      901 gcagttgctg gacgcgcagc gcctctacga gcaggcccgc ctcgggcgcg cgcgcgccct
      961 gggccagcgc ttctcggact gtgcccagtg gttcatcgcc atgggcgagg cggacgacac
     1021 gggcgccgcg ccggtccagg cgaccatgcg ccgcgagccg tgatcgcgac ggcgcggcgc
     1081 gggcgccggt tgcgccggct caagcccgca cgtgcgtgcg cttcgcagac tcgaggctcg
     1141 tccatccgcc gcggaccgcg tctggcgctc cgcatcgacc tcgcaaggag catcgcatgg
     1201 aacgcttgaa ggaccgggtc gcgctcgtga ccgggggagc catgggcatc ggcgagtcga
     1261 catgccggct gatggcgcgc gagggcgccg tcgtcgcggt gacggacctc gacgaggttc
     1321 gcggcgaggc ggtggccgcc tcgatccgtg ccgcgggtgg ccgcgcgggg ttctggcacc
     1381 tcgacgtggc ggaggaatcg caggtgcgcg aggtgttctc gaacgtgcag tctcgcctcg
     1441 gcgcgatccg cgtgctggtc aacaacgcgg gcatcgcggg ggccaacaag ccgacgcacg
     1501 agctgagcgt ggacgaatgg gagcgcgtga tggcggtgaa cgtgcgcggc gtcttcctct
     1561 gcaccaagca cgtcgtgccg cagatgaagt ccgccggcgg cggcagcatc gtcaacctct
     1621 cgagcatcta cggcctcgtg agcgcgcccg acgtcccgcc ctaccatgcg agcaagggcg
     1681 cggtgcgcct catgacgaag acggacgcgc tgctctacgc ggccgatcgc atccgcgtca
     1741 acagcatcca ccccggcttc atccgcacgc cgatggtcgc cggcttcatg cagacgcagg
     1801 ccgacccggc cgcggccgaa gccgcgaccg cgcagctgca cccgctcggc cacctcggcg
     1861 agcccgacga catcggctgg gcctgcgtgt acctcgcgag cgaggagagc cgcttcgtca
     1921 cgggctcgga gctggtggtg gacggcgggt acacctgtcg ttgagtcacg cgcccggcgc
     1981 gggcgcgggc gcgagccggc gaggcgtcac ggcggacatg tacagctcgc ggaccgcgag
     2041 cccgccatag ggcaggcgcg acgccgtgac ggtggcgacg tagtcggcgt aggcgtactg
     2101 gatccgcgcg accgcgaccg ggctcgagaa gagctgcacg aggctcgggt cgcccgtcgt
     2161 cgtggtcggg tggcccagcg cccgctgcag tccgtcgacc gcgccgcgcc agctctggcg
     2221 cgcctgcgat tcgtccatgc cgcggcggta caggttgatc gcgacgaggt ggccgcccgg
     2281 atcgaagctg aaccagatct cgctgaccgg tggcccctcg accccgagcg cgtcggccgc
     2341 gaccccgcgg cagcgcaggt agcggtagcc gcgggcgatg gcgtcgcaat gcgcccgggc
     2401 gctcgccatc cgctgcgcga cctggggctc cgtgctgcgg tcgagcttca gcccgagcgc
     2461 cggccgctcg ggcgcgagcg cgtcgcccct tgtccgcatg atggcgtcgt cgcgcacggc
     2521 gagcacctgg cgggcgtcga cggtgttgac cgggcagggc acgccgagcc gccccagcaa
     2581 ggcgcggccc ccggcggtgt gcatccagcc gatggaggcg gccgccaggg ccgccacggc
     2641 gatcgtcccg gcgacgacgc gcgcgcgtcg cggcgtcctc agctcgcgca tgatgtcgcg
     2701 accgtcggcg cgaggtagat cgggccggtc ggcgtcgccg tcgtgatggt gccgcgcagg
     2761 ttgaacaggg tcggcacgac gctgtactgg aacttcgtgc cgaccgcgat gcccgcggcg
     2821 ttggacgcgg tcagcgcata cgtcccgttc gtcgtcgagg tcagcgccgc gaagtcggcg
     2881 gccggcagtt gcgcctgcag cacgccgacg aggtcgacca tgaactggtc gaacacgcag
     2941 ccggggacgc cgacgtcgct gtgcgccgcc gtcatgtcct ggcacatctc gggctgcccg
     3001 tcggtgtcgc cggccacggt caccgggccc ggatagctgt acgggccgcc caggagcgac
     3061 tcgaactgca gcgtcaggca ggccttcagc cgcgcgggcg tgtcgtgcgc ggcgctgctc
     3121 ttgctggtgt tggacgtgac cgcgacgaaa tacggcgcct cgaccgggtc ggccagcacg
     3181 ccggtcaccg cggcgtcgac gacgcttgcc accgcggccg gcgcggacgc gtcgccgccg
     3241 agcgccgtcc agatcgccgc gttctcgggg tgctggacgg tggcgacggg cggatcggag
     3301 tcgccgccgc agccgcccaa tgccgccgcg gcgatcgcgg cgaaaaacga agtcgcgagt
     3361 ttcatgggat cgtctcctct gcaaggccgt cgaggcgccg ctgcccgtcg ctcgacgagg
     3421 cagcttgggc ggacggcaag gagttctacg ggcggcgccg cgaggcggat tcagccgccg
     3481 atcgatcccg cggcggacat gccggcgcgc ctcgaccggc ttggccgcgc ggcggcggct
     3541 acaggcccgc gagcgccccg gcccagcgcg cgagccggct ctcgaacacg aggcggccgt
     3601 cgaccgc
//
LOCUS       DASVLN010000031         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_431675, whole genome shotgun sequence.
ACCESSION   DASVLN010000031 DASVLN010000000
VERSION     DASVLN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431675"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..>1610
                     /locus_tag="VF453_00835"
     CDS             102..>1610
                     /locus_tag="VF453_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX7686212.1"
                     /translation="MPGVYRYFDVAGNVLYVGKARNLKRRVSSYFQKDHGGTRIGHMI
                     GRIARLETTVVRTEAEALLLENNLIKTLNPKFNILFRDDKSYPYLRLVTHAFPRVAYY
                     RGAVDRKHKYFGPYPGAWAVKETIQLIQKAFRLRTCEDTVFANRSRPCLLYQIQRCSG
                     PCVGLVSSEDYARDVRDAERFLLGDHKAVMDELQQAMMAHAEVFEYERAAEIRNRISA
                     LARVLHQQAMDESSLSARDKDVDILAVRVQGGRACVNLAMVRGGRHLGDRAYFPTHVE
                     DATAIDLGEDADAVDDAAGQAAAPVEPAVRVLEAFIAQHYLSSAPPPQWVTSHAVSKS
                     LVEALSLQTGMKIAVQHNPREQRRIWLEMCEKGAELALGRLLAEEGSQRERTRALADA
                     IDLAPDDLDALRIECFDISHTAGEATQASCVVYEGHKMQGSQYRRFNITGITGGDDYA
                     AMRQVLTRRYARFAVTPGAAGRGFEDVQSASTADAAADASIANDPQALAEAQA"
BASE COUNT          252 a          593 c          552 g          213 t
ORIGIN      
        1 acgtcgtcgc ccgcgccgcc gaggaggccg accgcgctgc cgtcgaggcc gcgcgcgccg
       61 tgcgcgagcg cgtgctggcc gaggtggccg cgctgcccgc gatgccgggc gtctaccgct
      121 acttcgacgt cgccggcaac gtgctgtacg tcggcaaggc gcgcaacctc aagcgccgcg
      181 tctcgagcta tttccagaag gaccacggcg gcacgcgcat cggccacatg atcggccgca
      241 tcgcgcggct cgagaccacc gtcgtgcgca ccgaggccga ggcgctgctc ctcgagaaca
      301 acctgatcaa gacgttgaac ccgaagttca acatcctgtt ccgcgacgac aagagctatc
      361 cctacctgcg cctcgtcacg cacgcgttcc cgcgcgtggc ctactaccgc ggcgcggtcg
      421 acaggaagca caagtacttc gggccgtatc ccggcgcgtg ggcggtcaag gagacgatcc
      481 agctgatcca gaaggcgttc cgcctgcgca cctgcgagga caccgtcttc gccaaccgct
      541 cgcggccctg cctgctgtac cagatccagc gctgctcggg gccttgcgtc gggctggtgt
      601 cgagcgagga ctacgcgcgc gacgtgcgcg acgccgagcg cttcctgctc ggcgaccaca
      661 aggcggtgat ggacgagctc cagcaggcca tgatggcgca tgccgaggtc ttcgagtacg
      721 aacgcgccgc cgagatccgc aatcgcatct cggcgctcgc gcgggtgctg caccagcagg
      781 cgatggacga gtccagcctg tcggcgcgcg acaaggacgt cgacatcctc gccgtgcgcg
      841 tgcagggcgg gcgcgcgtgc gtcaacctcg cgatggtgcg cggcggccgc cacctcggcg
      901 accgcgcgta cttcccgacg catgtcgagg atgcgaccgc gatcgacctc ggcgaggacg
      961 cggacgccgt cgacgatgcc gcggggcagg ccgccgcgcc ggtcgaaccc gccgtgcgcg
     1021 tgctcgaggc cttcatcgcg cagcactacc tctcgagcgc gccgccgccg cagtgggtga
     1081 cgagccatgc ggtctcgaag tcgctcgtcg aggccctgtc gctgcagacc ggcatgaaga
     1141 tcgccgtcca gcacaacccg cgcgagcaac gccgcatctg gctcgagatg tgcgagaagg
     1201 gcgccgagct cgcgctcggc cggctgctcg cggaggaagg ctcgcagcgc gagcgcacgc
     1261 gcgcgctggc cgacgcgatc gacctcgcgc ccgacgacct cgacgcgctg cgcatcgagt
     1321 gcttcgacat cagccacacc gcaggcgagg cgacgcaggc gtcctgcgtg gtctacgaag
     1381 gccacaagat gcagggctcg cagtaccgtc gcttcaacat caccggcatc accggcggcg
     1441 acgactacgc ggcgatgcgg caggtgctca cgcgccgcta cgcgcgcttc gcggtcacgc
     1501 ccggggcggc cggccgtggc ttcgaggacg tgcagtccgc ctcgaccgcc gacgccgcgg
     1561 ccgacgcgtc gatcgccaac gatccgcagg cgctcgccga ggcccaggcc
//
LOCUS       DASVLN010000032         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_163212, whole genome shotgun sequence.
ACCESSION   DASVLN010000032 DASVLN010000000
VERSION     DASVLN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163212"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VF453_00840"
     CDS             <1..928
                     /locus_tag="VF453_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX7686213.1"
                     /translation="VELRIGTGAPVQATIRGGGEVVLAAGAIGSPQILQLSGIGDGAR
                     LQALGIAPRHELRGVGANLQDHLQVRAVFAVDGTRTLNTLSATLLGKARIAAEYALAR
                     SGPMSMAPSQLGAFTRSDPSRRWPNVQYHVQPLSLEAFGEPLHAFEAFTASVCNLNPE
                     SRGEVHVVSADPAQAPRIAPRYLATEGDRRVAADSLRLTRRIVAQPALAAFKPREVKP
                     GTKFQTDDELARLAGDIGTTIFHPVGTCRMGRADDADAVLDPRLRVCGVAGLRVADAS
                     VMPDITSGNTNSPTLMIAERAAEWVLADASRK"
     gene            1281..2039
                     /locus_tag="VF453_00845"
     CDS             1281..2039
                     /locus_tag="VF453_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX7686214.1"
                     /translation="MDLVIVAAASGLAGFVDAVVGGGGLVLVPALFATYPNAVPATLF
                     GTNKGGAIWGTAWAAVQFARRVTLARATMAWGMAGALLGALGGAWAVTMVSARPLKQA
                     LPLVLLAILVYTIKRKDLGREHEPRLQGGAEARAAGLIGLAIGFYDGFFGPGTGSFFV
                     FLFVRLLGYDFLHASAAAKRLNTATNFAALVLFAWTGHIWWQVAAVLAVANVAGSLLG
                     TRLALARGAGFVRGVFIVVVSALILKTAADAFLR"
     gene            complement(2048..2746)
                     /locus_tag="VF453_00850"
     CDS             complement(2048..2746)
                     /locus_tag="VF453_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686215.1"
                     /translation="MNDNDPAAAPRARPREPAPRRRAWRVAAITLLVVAGFAGMAWRV
                     VQAQRDQGTLQDIHALAAEHQARLDALSARYRGIDLATAIGTDALARPDGVDAGRAVL
                     RRYLQVLADEEREHADYAARSQRLMERLAHDPVSAALLGYERGHLDRAFRRPGRVFRA
                     QRAFADAVGALFDCVGSGRAHLQVSGGHWVADSDDLQGALRERYAGISRASDAVQAAV
                     AATRRQAAQDDASL"
     gene            complement(2809..>3395)
                     /locus_tag="VF453_00855"
     CDS             complement(2809..>3395)
                     /locus_tag="VF453_00855"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX7686216.1"
                     /translation="APLDIAPAAGPGTASAPVAVAAAQPNESTQAQPAVMPAPVPVPI
                     HSSHRTRPTEPATNSSVAHGGPGVANESAREERQAAAACETCGVVASITPIEVQGETN
                     GVGAVAGGVGGALIGSQIAGRGNHTLGGVLGAVGGGLLGNTIEKHERRTTVYDVHVRM
                     EDGSMRTVRQSHAPAVGEHVYVEGHTLRARTQQG"
BASE COUNT          428 a         1251 c         1231 g          485 t
ORIGIN      
        1 cgtcgagctg cgcatcggca ccggggcgcc cgtgcaggcg acgatccgcg gcggcggaga
       61 ggtcgtgctg gcggcgggcg cgatcggatc gccgcagatc ctgcagcttt cgggcatcgg
      121 cgatggcgcg cgcctgcagg cgctgggcat cgcgccgcgc catgagctgc gcggcgtcgg
      181 cgcgaacctc caggaccacc tgcaggtgcg cgcggtcttc gccgtcgacg gcacgcgcac
      241 gctgaacacg ctgtcggcca cgctgctggg caaggcgcgc atcgcggcgg agtacgcgct
      301 ggcgcgcagc ggcccgatga gcatggcgcc gtcgcagctg ggcgcgttca cgcgctccga
      361 cccgtcgcgc cgctggccca acgtgcagta ccacgtgcag ccgctgtcgc tggaggcctt
      421 cggcgagccg ctgcacgcgt tcgaggcctt cacggcgagc gtctgcaacc tcaacccgga
      481 gtcgcgcggc gaggtgcacg tcgtctcggc cgaccccgcg caggcgccgc gcatcgcgcc
      541 gcgctacctc gccaccgagg gcgaccgccg ggtcgccgcc gactcgctgc gcctcacgcg
      601 ccgcatcgtt gcccagccgg cgctggcggc gttcaagccg cgggaagtca agcccggcac
      661 gaagttccag accgacgacg aactcgcccg cctggccggc gacatcggca cgacgatctt
      721 ccatcccgtc ggcacgtgcc gcatggggcg cgccgacgac gccgacgcgg tgctcgatcc
      781 gcgcctgcgc gtgtgcggcg tcgcggggct tcgcgtcgcc gatgccagcg tgatgcccga
      841 catcaccagc ggcaacacca actcgccgac gctgatgatc gcggagcgtg cggcggagtg
      901 ggtgctcgcc gatgcctcca gaaaatgaac gcctcattaa cacggcgtga acgatgttgg
      961 tgcaacacat cgggaatgcc cgtattcgca acccatccgt gcgtccctac agtgcatata
     1021 ctttcatggc atatgcacca gtttggtgcg tctgccgctg aagggggtct gaggagacaa
     1081 aaagccggca cgcacgacct gcgagccagg caggcggcgc caagagcgac cagccacaca
     1141 acagaagaag cagaaaacga tgaagggcgc tgccgcgagg ccgcgccctt cgtcctttcg
     1201 ggccgccgcg gccgaagtag cagcgcgtat ccgcaccccg tgccgatccg gtcgccgccg
     1261 gccccgataa tgcccggatg atggatctcg tgatcgtggc ggcggcttcg ggcctcgccg
     1321 gcttcgtcga cgcggtggtg ggcggaggcg gcctcgtcct cgtgcccgcg ctgttcgcca
     1381 cctatcccaa tgccgttccg gcgacgctgt tcggcaccaa caagggcggc gcgatctggg
     1441 gcacggcgtg ggcggcggtg cagttcgcgc gccgcgtgac gctcgcccgg gcgacgatgg
     1501 cgtggggcat ggccggcgcg ctgctcggcg cgctgggcgg cgcatgggcg gtgacgatgg
     1561 tcagcgcgcg gccgctcaag caggcgctgc cgctggtgct gctggcgatc ctcgtctaca
     1621 cgatcaagcg caaggacctc ggccgcgagc acgaaccccg gctgcagggc ggtgccgagg
     1681 cccgcgccgc cggcctgatc ggcctggcca tcggcttcta cgacggcttc ttcggcccgg
     1741 gcaccggcag cttcttcgtg ttcctgttcg tgcggctgct gggctacgac ttcctgcacg
     1801 cctcggcggc ggccaagcgg ctcaacacgg cgaccaactt cgcggcgctc gtgctgttcg
     1861 cctggaccgg ccacatctgg tggcaggtcg ccgcggtgct ggccgtcgcc aacgtcgcgg
     1921 gcagcctgct cggcacgcgt ctcgcgcttg cgcgcggggc gggcttcgtg cgcggcgtgt
     1981 tcatcgtcgt cgtgtcggcg ctgatcctga agacggcggc ggacgcgttc ctgcgctgac
     2041 cggcgcgtca gaggcttgcg tcgtcctgcg cggcctggcg cctcgtggcc gcgaccgccg
     2101 cctgaacggc gtcggaggcc ctgctgatgc cggcgtagcg ctcccgcagc gcgccctgca
     2161 ggtcgtcgga gtcggcgacc cagtggccgc cggacacctg caggtgagcg cgcccggagc
     2221 cgacgcagtc gaacagcgcg ccgacggcat cggcaaacgc gcgctgcgcg cggaataccc
     2281 ttccaggccg gcggaacgcc ctgtccaggt gcccgcgttc ataaccgagc agcgcggcgc
     2341 tcaccggatc gtgggccagt cgctccatca gacgctgcga ccgcgcggcg tagtccgcat
     2401 gctcgcgctc ctcgtcggcg agcacctgca ggtagcgccg cagcaccgcc cggccggcat
     2461 cgacgccatc cggacgcgcg agcgcgtcgg tgccgatcgc ggtcgcgaga tcgatcccgc
     2521 ggtagcgcgc ggacagcgcg tcgaggcgcg cctggtgctc cgcggcgagc gcgtggatgt
     2581 cctgcagcgt gccctggtcg cgctgtgcct ggacgacgcg ccaggccatg cctgcgaagc
     2641 cggcgaccac cagcagcgtg atcgcggcga cgcgccaggc gcgcctgcgc ggcgccggct
     2701 cgcgcggcct cgcccgcggc gcggccgccg gatcgttgtc gttcatggcg cggtgtcctc
     2761 ctccggcgcc gattctagtg atcggcgccg gcgcgacgac cgcgcccgtc agccctgttg
     2821 cgtccgcgcc cgcagcgtgt ggccctcgac gtagacgtgc tcgcccaccg ccggcgcatg
     2881 cgactgtcgc accgtgcgca tcgagccgtc ctccatccgg acgtggacgt cgtagaccgt
     2941 ggtgcggcgt tcgtgcttct cgatcgtgtt gcccagcagg ccgccgccca ccgcgccgag
     3001 cacgccgccc agcgtgtggt tgccgcggcc ggcgatctgg ctgccgatca gggcgccgcc
     3061 cacgccgccg gccaccgcgc cgacgccgtt ggtctcgccc tgcacctcga tcggcgtgat
     3121 cgaggcgacg acgccgcagg tctcgcatgc ggccgccgcc tggcgttcct cgcgtgcaga
     3181 ctcgttggcc acgcccggcc cgccgtgggc gaccgaggag ttcgtcgccg gctccgtcgg
     3241 gcgcgtgcgg tgcgaggagt ggatcggcac cggcacgggc gccggcatca cggcgggctg
     3301 cgcctgggtc gattcattgg gttgcgccgc cgcgacggcc accggcgcgg aggccgtgcc
     3361 cggcccggcg gccggagcga tgtccagcgg cgcgc
//
LOCUS       DASVLN010000033         6467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_163225, whole genome shotgun sequence.
ACCESSION   DASVLN010000033 DASVLN010000000
VERSION     DASVLN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163225"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..6142
                     /locus_tag="VF453_00860"
     CDS             <1..6142
                     /locus_tag="VF453_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX7686217.1"
                     /translation="LAAAALGALAPVAAQAQAPATLKTFSPRGEVQLVRQVRATFAES
                     MVKFGDPRLPAPMDVSCAPALGGSARWVDDKTWVFDFAKDVPPGTRCNVTPRAGFKSV
                     AGAAIAADAKFAFSTGGPAIVRAYPEPDGNQIEEEQAFALLLNGPATADSVRRFAYCE
                     VSGLGERLPVALVTGPTRDAILKAVHLEAQKDRAVTLRCSRPVVPQAKLTLVWGAGVA
                     TASGVPTTADRRLDYQVRPAFTASFTCERVNSRSDCLPMRPLRLEFSAPVPRRIAERV
                     VLVGPDGKHAAQVEQHRGEVRDALVETRQSGFSKFLYFWNRSKGEAGGDASEEGVTAV
                     QFSGTLPEKADLRIELPADFADDSGRKLENADQFPLATRTSDAPALAKFPAATFGILE
                     LNADPVLPLTVRKIEGDLGIQALTLAGAPARDLALTDDRQVIEWFARVARYDETRLDR
                     ATVQDELGVNLPPAVAPPKPARKAKSYGRDKTDDEQEEDAGDDNAPFDPNVIQTREVS
                     LLASVKEARALKLPSDQRTDPHPFEVVGIPLPQPGYHVVEIASPRLGQSLLDRAAPMY
                     VRTGVLVTNLAVHFKWAPVNSGAWVTTLDKGQPVPGASIQVSDCRGVKVWSGRTDAQG
                     FARIDQALPRLDTSDYCSRTGEGRWQGSSGRPSGYFVSARKTDDQGRADMAFVWSTWQ
                     DGIEPWRFNLRSGGYGYGAGNAVSRALFHTVMDRTLLRAGQTISMKSHARRELLAGLA
                     LVDPHELPPTLRIEHEGTGEHVDFPLAWRKGRYAETTWKIPEDAKLGVYDVSLVDARH
                     GQVQTGSFRVEEFRLPVMTGRLVPPAGPQVDPRELALDVMVNYGNGGGAAGLPLRVSA
                     QVRDIDLDQAIPTQRWPGFHFEPPKAPADAAAANDGEGLFQQDYVDEDDADTMVQQRD
                     PHSKLVADKLPVTLDKSGAGKVTLTKLPPTVAPKQLLVQATYADPNGEVQTLSQTLPL
                     WPSAVVLGVRTDDWVSVHQKLPAQVVALDTAGKPMAGVAVSVRAVVHHQLTARKRLVG
                     GFYAYDSRSIDEDLGEVCSGRSDARGLVLCEVELSAAGNVELIAEAKDAQGHAARAAS
                     SVWVTRAGEVWFGSANDDRMDVLPEQRSYEPGQVARFQVRSPFRHATALLAIERNGIL
                     ETRVVDLDGRDPTIEVPVKAEWSPNVFVSVLAVRGRVREVPWYSFFSWGWKSPVEWWH
                     AWRDEGQEYQPPTAMVDLAKPAFRYGIAEIEVGSAAHRLKVDVVPDKPTYPIRATSQV
                     RVKVALPDGRPAPAGTEVTLAAVDEALLTLMPNDSWDLFDAMIQRRDYAVETATAQMQ
                     VIGKRHFGKKAAPPGGGGGGGQFPTRELFDTLLLWNPTVTLDARGEAVVPVPLNDSLT
                     SFRIVAVADVVQGADAALFGTGQATIAATQDLQIVSGVPPLVREGDHYKAMFTLRNTT
                     KQAMQASLNGQVGDAKLPALTVTIPAAGAVDTGWDVDAPFNVKHVDWVVAADAGSAHD
                     RMKFAQKIAEAVPVTVQQATLVQVDKALRMPIAPPANALADAGGKLRGGIEVALAPRL
                     GDGLAGVRDYFARYPFSCLEQQASVDVGLRDTAAWSAMARRIPLYLDEDGLADYFPQS
                     GGRPHSGSDALTAYLLAISDEASRLGYDFSLPDDVREQMLRGLIGFVEGRVKRDDFWK
                     PAFLKNGDLDVRKLAAIEALSRYGKAQPRMLDSVQVLPNQWPTGAVIDWLQILQRLDA
                     IPDRAKKIDEAEQILRARLNVQGTRLGFSTERDDSWWWLMVNGDVNATRMILAVLDRP
                     GWQDDEPRIVTGTLQRQQYGHWSTTTANAWGSVAMEAFSKKFESTPVRGATRATFGAP
                     SPTQTLDWSRNATGGTLALGWPQGFAVNGNKADANLQVDHEGTGKPWVTVTSRAAVPV
                     TVPFGSGYRITKTVTAVEQKVAGKWSRGDVARVHLDIDVQADATWVVIDDPVPGGGTL
                     LGSGLGGDDQISTGTEKDDQRGWLAYQERSFEAFRAYYRYLPKGSFSVEYTLRLNNPG
                     RFGLPQTRVEAMYAPEMFGESPNAAWEVQP"
     gene            6139..>6467
                     /locus_tag="VF453_00865"
     CDS             6139..>6467
                     /locus_tag="VF453_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7686218.1"
                     /translation="MIRRPRALVLALAAAGLAASAAHAGEPSFEQVRAAYRSSQTVIL
                     DRHGAPVQSVRTDTHARRGAWVPLAQISPAMRAAAIASEDQRFLAHAGVDWQAVAGAA
                     IGRADAGG"
BASE COUNT          937 a         2315 c         2380 g          835 t
ORIGIN      
        1 gctggcggcg gcggcgctgg gtgcgctggc gcccgtcgcg gcgcaggcgc aggcgccggc
       61 gacgctcaag accttctcgc cgcgcggcga ggtgcagctg gtgcgccagg tgcgcgcgac
      121 cttcgccgag tccatggtca agttcggcga tccgcggctg ccggcgccga tggacgtctc
      181 gtgcgcgccc gcgctgggcg gcagcgcgcg ctgggtcgac gacaagacct gggtgttcga
      241 cttcgccaag gacgtcccgc ccggcacgcg ctgcaacgtc acgccgcgcg ccggcttcaa
      301 gtcggtcgcg ggcgcggcga tcgcggcgga cgcgaagttc gcgttctcga ccggcggccc
      361 ggccatcgtg cgtgcctatc cggagccgga cggcaaccag atcgaggagg agcaggcctt
      421 cgcgctgctg ctcaacggac cggccacggc cgacagcgtc cggcgcttcg cctactgcga
      481 ggtctcgggc ctgggcgaac gcctgcccgt ggcgctggtc accggcccga cgcgcgacgc
      541 catcctgaag gccgtgcatc tcgaggcgca gaaggaccgc gcggtgacgc tgcgctgctc
      601 gcggcccgtc gtgccgcagg ccaagctcac gctcgtgtgg ggcgcgggcg tcgcgacggc
      661 cagcggcgtg cccacgaccg ccgaccgccg cctcgactac caggtgcgcc cggccttcac
      721 ggccagcttc acctgcgagc gcgtgaattc gcgctcggac tgcctgccga tgcggcccct
      781 tcggctggag ttctccgcgc cggtgccgcg gcgcatcgcg gagcgggtcg tcctcgtcgg
      841 gcccgacggc aagcacgccg cccaggtcga gcagcaccgc ggcgaggtgc gcgacgcgct
      901 cgtcgagacg cggcaatcgg gcttcagcaa gttcctgtac ttctggaacc gcagcaaggg
      961 cgaggccggc ggcgacgcct cggaggaagg cgtgacggcc gtgcagttct ccggcacgct
     1021 gcccgagaag gccgacctgc gcatcgagct gcccgccgac ttcgccgacg actcgggccg
     1081 caagctcgag aacgccgacc agttcccgct cgcgacgcgc acctcggacg cgccggcgct
     1141 cgccaagttc ccggccgcga ccttcggcat cctggagctc aacgccgacc cggtgctgcc
     1201 gctgaccgtg cgcaagatcg agggcgacct cggcatccag gcgctcacgc tggccggcgc
     1261 gcccgcgcgc gacctcgcgc tgaccgacga ccgccaggtc atcgagtggt tcgcgcgcgt
     1321 tgcccgctac gacgagacgc gcctcgatcg cgcgacggtg caggacgagc tcggcgtcaa
     1381 cctgccgccg gccgtcgcgc cgccgaagcc ggcgcgcaag gcgaagtcct acggccgcga
     1441 caagaccgac gacgaacagg aggaggacgc gggcgacgac aacgcgccct tcgatccgaa
     1501 cgtgatccag acgcgcgagg tctcgctgct ggcgagcgtc aaggaggcgc gtgcgctcaa
     1561 gctgccgagc gaccagcgca ccgacccgca tccgttcgag gtcgtcggca tcccgctgcc
     1621 gcagcccggc taccacgtcg tcgagatcgc ctcgccgcgg ctcgggcagt cgctgctcga
     1681 tcgcgcggcg cccatgtacg tgcgcaccgg cgtgctcgtg accaacctcg ccgtgcactt
     1741 caagtgggcg ccggtgaact cgggcgcctg ggtgacgacg ctggacaagg gccagcccgt
     1801 gcccggcgcg tcgatccagg tctccgactg ccggggcgtg aaggtctgga gcggccgtac
     1861 cgacgcccag ggcttcgcgc gcatcgacca ggccctgccg cgcctggata cgagcgacta
     1921 ctgcagccgc acgggcgagg ggcgctggca gggctcgtcg ggcaggccca gcggctactt
     1981 cgtcagcgcc cgcaagacgg acgaccaggg ccgcgccgac atggccttcg tgtggtcgac
     2041 ctggcaggac ggcatcgagc cgtggcgctt caacctgcgc tcgggcggct acggctacgg
     2101 cgccggcaac gcggtctcgc gcgcgctgtt ccacacggtg atggatcgca cgctgctgcg
     2161 cgccggccag acgatctcga tgaagagcca cgcgcgccgc gagctgctcg ccggactggc
     2221 gctcgtcgac ccgcacgagc tgccgccgac cctgcgcatc gagcacgagg gcaccggcga
     2281 gcacgtcgat ttcccgctcg cctggcgcaa gggccgctac gccgagacga cgtggaagat
     2341 ccccgaggac gcgaagctgg gcgtctacga cgtctcgctc gtcgacgccc gccacggcca
     2401 ggtgcagacg ggctcgttcc gcgtcgagga gttccgcctg ccggtgatga ccggccgcct
     2461 cgtgccgccg gccgggccgc aggtcgatcc gcgcgagctg gcgctggacg tgatggtcaa
     2521 ctacggcaac ggcggcggtg ccgccggcct gccgctgcgc gtgtcggcgc aggtgcgcga
     2581 catcgacctc gaccaggcca tcccgaccca gcgctggccg ggcttccact tcgagccgcc
     2641 caaggcaccg gccgacgcgg cggcggccaa cgacggcgag gggctgttcc agcaggacta
     2701 tgtcgacgag gacgacgccg acacgatggt gcagcagcgc gatccgcact cgaagctggt
     2761 cgccgacaag ctgcccgtca cgctcgacaa gtccggggcg ggcaaggtca ctctgaccaa
     2821 gctgccgccc accgtggcgc ccaagcagct gctggtgcag gcgacctatg ccgacccgaa
     2881 cggcgaggtg cagacgctca gccagacgct gccgctgtgg ccgtccgcgg tcgtgctggg
     2941 cgtgcgcacg gacgactggg tctccgtgca ccagaagctg ccggcgcagg tcgtggcgct
     3001 ggacaccgcg ggcaagccga tggccggcgt cgcggtgagc gtgcgcgcgg tggtgcatca
     3061 ccagctgacg gcgcgcaagc ggctggtcgg cggcttctac gcctacgaca gccggagcat
     3121 cgacgaggat ctcggcgagg tctgctccgg caggagcgac gcgcgcggcc tcgtgctgtg
     3181 cgaggtcgag ctctcggccg cgggcaacgt ggagctcatc gccgaggcca aggacgcaca
     3241 gggccatgcc gcgcgcgcgg cgtcctccgt gtgggtgacg cgcgccggcg aggtctggtt
     3301 cggcagcgcc aacgacgacc gcatggacgt gctgccggag cagcgctcgt acgagccggg
     3361 ccaggtcgcg cgcttccagg tgcgcagtcc gttccgtcac gcgaccgcgc tgctcgcgat
     3421 cgagcgcaac ggcatcctcg agacgcgcgt cgtcgacctc gacggccgcg acccgacgat
     3481 cgaggtgccg gtcaaggccg agtggtcgcc caacgtcttc gtctcggtgc tggccgtgcg
     3541 cgggcgggtg cgcgaggtgc cgtggtactc gttcttcagc tggggctgga agtcgccggt
     3601 cgaatggtgg catgcatggc gcgacgaagg ccaggagtac cagccgccca ccgcgatggt
     3661 cgacctcgcc aagcccgcgt tccgctacgg catcgccgag atcgaggtcg gcagcgcggc
     3721 gcaccggctg aaggtcgacg tcgtgcccga caagccgacc tacccgatcc gcgcgaccag
     3781 ccaggtgcgc gtgaaggtcg cgctgcccga cggccgtccc gcgcccgcgg gcacggaggt
     3841 gacgctcgcc gccgtcgacg aggcgctgct cacgctcatg cccaacgaca gctgggatct
     3901 cttcgacgcg atgatccagc gccgcgacta cgccgtggag acggcgaccg cgcagatgca
     3961 ggtgatcggc aagcgccact tcggcaagaa ggccgcgccc ccgggcggcg gcggcggcgg
     4021 cggccagttc cccacgcgcg agctgttcga cacgctgctg ctgtggaacc cgaccgtcac
     4081 gctcgacgcc cggggcgagg ccgtcgtgcc cgtgccgctg aacgactcgc tcacgagctt
     4141 ccgcatcgtc gcggtcgccg acgtcgtgca gggcgcggac gcggcgctgt tcggcacggg
     4201 ccaggcgacg atcgcggcga cgcaggacct gcagatcgtc tccggcgtgc cgccgctcgt
     4261 gcgcgagggc gaccactaca aggccatgtt cacgctgcga aacacgacga agcaggcgat
     4321 gcaggcgagc ctgaacggcc aggtcggcga cgcgaagctg ccggcgctga cggtgacgat
     4381 cccggcggcc ggcgcggtcg acacgggctg ggacgtcgac gcgccgttca acgtgaagca
     4441 cgtcgactgg gtcgtcgccg ccgacgcggg ctcggcgcac gaccgcatga agttcgcgca
     4501 gaagatcgcc gaggcggtgc cggtgacggt gcagcaggcg acgctcgtgc aggtcgacaa
     4561 ggcgctgcgc atgccgatcg cgccgccggc caacgcgctc gccgacgcgg gcgggaagct
     4621 gcgcggcggc atcgaggtgg cgctcgcgcc gcgcctgggc gacggcctgg ccggcgtgcg
     4681 cgactacttc gcgcgctatc cgttctcctg cctcgagcag caggcctccg tcgacgtggg
     4741 cctgcgcgac accgcggcct ggtcggcgat ggccaggcgc atcccgctgt acctcgacga
     4801 ggacgggctc gcggactact tcccgcagtc cggcggccgc ccgcattcgg gcagcgacgc
     4861 gctgaccgcc tacctgctcg cgatcagcga cgaggcgagc cgcctgggct acgacttctc
     4921 gctgcccgac gacgtgcgcg agcagatgct gcgcgggctg atcggcttcg tcgagggccg
     4981 cgtcaagcgc gacgacttct ggaagccggc cttcctgaag aacggcgacc tggacgtgcg
     5041 caagctcgcc gcgatcgagg cgctgagccg gtacggcaag gcgcagccgc gcatgctcga
     5101 ctcggtgcag gtgctgccca accagtggcc caccggcgcg gtgatcgact ggctgcagat
     5161 cctgcagcgc ctggacgcga tccccgaccg cgcgaagaag atcgacgagg ccgagcagat
     5221 cctgcgcgcg cgcctgaacg tgcagggcac gcggctcggg ttctcgaccg agcgcgacga
     5281 cagctggtgg tggctgatgg tcaacggcga cgtcaacgcg acgcgcatga tcctggccgt
     5341 cctcgatcgt ccgggttggc aggacgacga gccgcgcatc gtcaccggca cgctgcagcg
     5401 ccagcagtat ggccactggt cgacgacgac ggccaatgca tggggcagcg tggcgatgga
     5461 ggcgttctcg aagaagttcg agtcgacccc tgtccgcggc gcgacgcgtg ccaccttcgg
     5521 cgcgccgagc ccgacgcaga cgctggactg gtcgaggaac gcgacgggcg gcaccctggc
     5581 gctcggctgg ccgcagggct tcgccgtcaa cggcaacaag gccgacgcca acctgcaggt
     5641 cgaccacgaa ggcaccggca agccgtgggt caccgtgacc tcgcgcgcgg cggtccccgt
     5701 gaccgtgcct ttcggcagcg gctaccgcat caccaagacg gtgacggcgg tggagcagaa
     5761 ggtcgcgggc aagtggtcgc gcggcgacgt ggcgcgggtg cacctggaca tcgacgtgca
     5821 ggccgacgcg acgtgggtcg tgatcgatga cccggtcccg ggcggcggca cgctgctcgg
     5881 aagcggactg ggcggcgacg accagatcag caccggcacg gagaaggacg accagcgcgg
     5941 ctggctcgcc taccaggagc gcagcttcga ggccttccgt gcgtactacc gctacctgcc
     6001 caagggctcg ttcagcgtcg agtacacgct gcgcctgaac aacccgggcc gcttcgggtt
     6061 gccgcagacc cgcgtcgagg cgatgtacgc gcccgagatg ttcggcgagt cgcccaacgc
     6121 ggcgtgggag gtgcagccgt gatccggcgt ccgcgcgcgc tcgtgctggc gctcgcggct
     6181 gcggggctgg cggcgtcggc ggcgcatgcc ggggagccct ccttcgagca ggtgcgcgcg
     6241 gcgtaccgga gctcgcagac ggtgatcctc gatcgccacg gcgcgcccgt gcagtcggtg
     6301 cgcaccgaca cgcatgcgcg gcgcggcgcc tgggtgccgc tggcgcaaat ctcgccggcg
     6361 atgcgggcgg cggcgatcgc gtcggaggac cagcgcttcc tcgcgcatgc cggcgtcgac
     6421 tggcaggccg tggccggcgc ggcgatcggg cgcgccgatg cgggcgg
//
LOCUS       DASVLN010000034         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_216741, whole genome shotgun sequence.
ACCESSION   DASVLN010000034 DASVLN010000000
VERSION     DASVLN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216741"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VF453_00870"
     CDS             <1..1046
                     /locus_tag="VF453_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7686219.1"
                     /translation="AAPAQPLDARQLAKLALAAQREIAQRCERQFAEACRDLRRTNDK
                     VLTDPAAAAADAQQLARALLDKMLVEGEPCLRVLGEMSSDRHAAHAMNVTVISLMLAR
                     QLSVPQPVLMALGVGALMHDVGKIALPDRVRLAREDFNASEAALYRDHVAQGLILGRR
                     MGLAPDAQLVIAQHHEHADGTGFPSALMLPRMSLPARIVALVNRYDNLCNAANPAHSI
                     TPHEALSRLFAQSRSKFDETLLSGFIKMMGIYPPGSVVQLNDDRYAMVMTVNAAHPLK
                     PRVLVYETGVPVDEALHVDLEREPLLGIRRSLKPAQLPTRAHDYLKPRQRVSYFFDVE
                     SAPKEEPHEEVTA"
     gene            1075..>1898
                     /locus_tag="VF453_00875"
     CDS             1075..>1898
                     /locus_tag="VF453_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7686220.1"
                     /translation="MTPAAPLLTAVAARPAFSGVLALLAAIGEPAWVVDAARCCVLAA
                     NLEAEELFGRGELEGLDAEDVIPDLEDIAYWDGVRCGSVGTLETEAELHLPGGRTLAV
                     VRRIAPLWLADGTALYLVQVRDRTHERRVERERETALAELRATLEATADAILVTDLAG
                     RIRGFNRRFAALWAIPDAALHAADDRAVHAWLRMNVMDVDAYDRRLEDVFAHTLAEAR
                     DEIAHVNGAIIERHAQPQWSQGRPIGRVFSFRELNPRRCGAPQPAGHDGVDQLTALP"
BASE COUNT          264 a          715 c          684 g          235 t
ORIGIN      
        1 ccgccgcgcc cgcgcaaccg ctggacgccc gccagctcgc caagctggcg ctggcggcgc
       61 agcgcgagat cgcccagcgc tgcgagcgcc agttcgccga ggcctgccgc gacctgcggc
      121 gcaccaacga caaggtgctg accgaccccg ccgcggccgc cgcggacgcc caacagctgg
      181 cccgggcgct gctggacaag atgctggtcg aaggcgagcc ctgcctgcgt gtgctgggcg
      241 agatgtccag cgaccgccac gccgcgcacg cgatgaacgt gacggtgatc tcgctgatgc
      301 tggcgcgcca gctctccgtg ccgcagccgg tgctgatggc gctgggcgtg ggcgcgctga
      361 tgcacgacgt cggcaagatc gcgctgcccg atcgcgtgcg cctcgcgcgc gaggacttca
      421 acgccagcga ggccgcgctg taccgcgacc acgtggcgca gggactgatc ctcggccggc
      481 ggatggggct cgccccggac gcgcagctcg tgatcgcgca gcaccacgag cacgccgacg
      541 gcaccggctt cccgagcgcg ctcatgctgc cgcggatgtc gctgccggcg cgcatcgtgg
      601 cgctggtcaa ccgctacgac aacctgtgca acgccgccaa cccggcgcat tcgatcacgc
      661 cgcacgaggc cctgtcgcgc ctgttcgcgc agagccgctc gaagttcgac gagacgctgc
      721 tctccggctt catcaagatg atgggcatct acccgccagg gtcggtggtg cagctcaacg
      781 acgaccgcta cgcgatggtc atgaccgtca acgccgcgca cccgctcaag ccgcgcgtgc
      841 tggtgtacga gacgggcgtg ccggtcgacg aggcgctgca cgtcgacctc gagcgcgagc
      901 cgctgctggg catccgccgc agcctgaagc cggcccagct gccgacgcgg gcgcacgact
      961 acctcaagcc gcgccagcgc gtgtcgtact tcttcgacgt cgagagcgcg ccgaaggaag
     1021 agccgcacga ggaggtgacg gcatgactgc tgcccgccgc gcggaggccg cgcgatgacg
     1081 ccggccgccc cgctgctgac cgcggtcgcg gcgaggcccg ccttctccgg cgtcctggcg
     1141 ctgctggccg cgatcggcga gccggcgtgg gtggtcgacg ccgcgcgctg ctgcgtgctg
     1201 gccgccaacc tcgaggcgga ggagctcttc ggccgcggcg agctcgaggg cctggacgcc
     1261 gaggacgtga tccccgacct cgaggacatc gcctactggg atggcgtgcg ctgcggcagc
     1321 gtgggcacgc tcgagaccga ggcggagctg cacctgccgg gcgggcgcac gctggccgtc
     1381 gtccgccgca tcgcgccgtt gtggctcgcc gacggcaccg cgctgtacct ggtgcaggtg
     1441 cgcgaccgca cgcacgagcg gcgcgtcgag cgcgagcgcg agaccgcgct ggccgagctc
     1501 cgggcgacgc tcgaggcgac cgcggacgcg atcctcgtga ccgacctggc gggccgcatc
     1561 cgcggcttca accgccgctt cgcggccctg tgggcgattc ccgacgcggc cctgcacgcc
     1621 gccgacgacc gcgccgtgca cgcatggctg cggatgaacg tgatggacgt cgacgcctac
     1681 gaccgccggc tcgaggacgt cttcgcgcac acgctggccg aggcgcgcga cgagatcgcc
     1741 cacgtcaacg gcgccatcat cgagcgccac gcgcagccgc agtggagcca ggggcggccg
     1801 atcggacgcg tgttctcgtt ccgcgagctc aacccgcggc gctgcggcgc gccgcagccg
     1861 gccggccacg acggcgtcga ccagctcacc gcgctgcc
//
LOCUS       DASVLN010000035         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_324216, whole genome shotgun sequence.
ACCESSION   DASVLN010000035 DASVLN010000000
VERSION     DASVLN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324216"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..393
                     /gene="rnpB"
                     /locus_tag="VF453_00880"
     ncRNA           59..393
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF453_00880"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            452..1669
                     /locus_tag="VF453_00885"
     CDS             452..1669
                     /locus_tag="VF453_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX7686221.1"
                     /translation="MNPEAATLWRRPRWSLAALLACLGMLGAFSIDTYIPAFSSIGRA
                     LDATPVQMQQTLSAYLLGFAVMNLFHGALSDSLGRRPVVLWGIAIFTLASLGCALSQT
                     VGHLIFFRLVQGMSSGASMVVARAIIRDMFPPDEAQRVMSQVTIFFGVAPAIAPVFGG
                     LLLVHFGWHSVFVFLTFVGATLWTLSWRVLPETLHPTQVQPMHVGHLMRGYWQMGSDP
                     RFLALAFASGVPFNGMFLYILSAPVFLGDLLHLPPTRFFFLFLFTIGGIMGGAYFSGR
                     LAGKMPARRQIAIGFTIMFGIATLNLLMNLLVPARAPWAIPPIGIFSFGWALMVPVVT
                     LLVLDQVPQRRGMASSLQACIGSLANAFVAGVISPLVMHSTVALAAASLAMSCVGIVA
                     WIYVRNRLAVAPS"
     gene            1759..>2035
                     /locus_tag="VF453_00890"
     CDS             1759..>2035
                     /locus_tag="VF453_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686222.1"
                     /translation="MRHRLTLSVAAALALAATSARAEPAGPAVVVGQLGGMPTSPAFR
                     MDMNKGKTGAPEPTRLGAMPIETKASAAEAAAADKGREAKPSKAGEPA"
BASE COUNT          263 a          742 c          704 g          326 t
ORIGIN      
        1 gcggcggctg accctgcgcc cgcgcgggcg accgcgcccg tgggacaatc cgccccgtga
       61 agcgagccag accgccgctg gcacaccggc cgaaaggccg tgctggagga aggtccggac
      121 tgcacagggc agcgcagcag ctaacggctg tccgccgaca acccgcaagg gcctaaggcg
      181 aggatcagag caacagagac gagccgattc agttcgggtg aaacgggcaa tctctgcgcg
      241 cagcaacatc aagtaggcgg acgccgaccc ggcccgggga gtccgcgggt agatggcatc
      301 gagccgtcct ggcgacaggc ggcccagacg aatggcggtc acgcgcccgg cgacgggcgc
      361 gcacagaatc cggcctatcg gctcgcttca ctcttaatcg cgcgcggcgc ccggccgggg
      421 acgacgcgcc tggagccgcc gtcctcgccg catgaatcct gaagccgcaa ccctctggcg
      481 ccgaccgcgc tggagtctcg ccgccctgct cgcctgcctg ggcatgctgg gcgcgttctc
      541 gatcgacacc tacatccccg cgttctccag catcggccgc gcgctcgacg cgacgccggt
      601 gcagatgcag cagacgctct cggcctacct gctgggcttc gccgtgatga acctgttcca
      661 cggcgcgctg tcggacagcc tcggccgccg tccggtcgtg ctgtggggga tcgcgatctt
      721 cacgctcgcc tcgctgggct gcgcgctgtc gcagacggtc gggcacctca tcttcttccg
      781 gctggtgcag ggcatgagct cgggcgcgag catggtcgtg gcgcgcgcca tcatccgcga
      841 catgttcccg cccgacgagg cgcagcgcgt gatgtcgcag gtgacgatct tcttcggcgt
      901 cgcccccgcg atcgcgccgg tgttcggcgg cctgctgctg gtgcatttcg gctggcattc
      961 ggtgttcgtg ttcctgacct tcgtcggcgc gacgctgtgg acgctcagct ggcgcgtcct
     1021 gcccgagacg ctgcacccga cccaggtgca gccgatgcac gtcggccacc tgatgcgcgg
     1081 atactggcag atgggctcgg atccgcgctt cctcgcgctc gcgttcgcct cgggcgtgcc
     1141 gttcaacggc atgttcctct acatcctgtc ggcgccggtg ttcctcggcg acctgctgca
     1201 cctgccgccg acgcgcttct tcttcctgtt cctgttcacc atcggcggga tcatgggcgg
     1261 cgcgtacttc tcgggccggc tggcgggcaa gatgccggcg cgccggcaga tcgcgatcgg
     1321 cttcaccatc atgttcggca tcgcgaccct caacctgctg atgaacctgc tcgtgccggc
     1381 gcgcgcgccg tgggcgatcc cgccgatcgg catcttctcg ttcggctggg cgctgatggt
     1441 gccggtcgtg accttgctgg tgctcgacca ggtgccgcag cggcgcggca tggcctcgtc
     1501 gttgcaggcc tgcatcggca gcctggccaa cgcgttcgtg gcgggcgtga tctcgccgct
     1561 cgtcatgcac tcgacggtcg cgctggcggc ggcgtcgctg gcgatgtcgt gcgtgggcat
     1621 cgtcgcctgg atctacgtgc gcaaccggct ggccgtggcg ccgagctgac gcgccggccg
     1681 cgacgcggcg cgcggctcaa gtccggctcg cagcggccga tcacaagggg gcatcccgtc
     1741 cgtgatccgc ccaaggccat gcgtcatcgc ctcacccttt ccgtcgccgc cgcgctcgcg
     1801 ctggccgcca cgtccgcccg cgccgagccg gcgggccccg ccgtcgtcgt cggccagctc
     1861 ggcggcatgc cgacctcgcc ggcgttccgc atggacatga acaagggcaa gaccggcgcg
     1921 cccgagccga cgcggctggg cgcgatgccc atcgagacca aggcctccgc cgccgaggcc
     1981 gccgcggcgg acaagggcag ggaggcgaag ccctccaagg ccggcgagcc tgcgg
//
LOCUS       DASVLN010000036         4334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_485259, whole genome shotgun sequence.
ACCESSION   DASVLN010000036 DASVLN010000000
VERSION     DASVLN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485259"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VF453_00895"
     CDS             <1..511
                     /locus_tag="VF453_00895"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7686223.1"
                     /translation="AMGLGAWLRSRGVPLSPVPEAQAGTPGPGAPAQGAIPVPLTTLE
                     GRTLTAADIGHRSVVLNFWAPWCPPCVREMPEIDRFSRSAAGKDTLVIGLAIDEKPAV
                     DKFLAEHPVGFPIVVLGYSGLSWVRQLGNDSQALPFSVVFDRGGHKVVQRKAGATTAG
                     ELQGWVAKL"
     gene            745..1188
                     /gene="aroQ"
                     /locus_tag="VF453_00900"
     CDS             745..1188
                     /gene="aroQ"
                     /locus_tag="VF453_00900"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX7686224.1"
                     /translation="MNVLVLHGPNLNLLGLREPAVYGSATLPDIEQQLKQIAAEAGAR
                     LESLQSNHEGVLIDRVHAARTDGTDFIVINPAGFTHSSVALRDALAAVAIPFVEVHLS
                     NIHRREPFRHHSYFSDLAEGVICGLGAQGYTLALRFALSRAPVRD"
     gene            1266..1739
                     /gene="accB"
                     /locus_tag="VF453_00905"
     CDS             1266..1739
                     /gene="accB"
                     /locus_tag="VF453_00905"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX7686225.1"
                     /translation="MDMRKLKTLVDLVSESNISELEITEAEGKVRIVKGGYGGVPATM
                     MMQPTAPMMMPTQAQAQPAAAAEPAAGAAPEAPAITGHVVKSPMVGTFYRSSSPGSKA
                     FVEIGSQVKEGDPVCIVEAMKIMNEIEADMAGTISKILVENGQAVEYGQPLFIIE"
     gene            1773..3122
                     /gene="accC"
                     /locus_tag="VF453_00910"
     CDS             1773..3122
                     /gene="accC"
                     /locus_tag="VF453_00910"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7686226.1"
                     /translation="MFKKILIANRGEIALRIQRACREMGIQSVVVYSEADRDAKYVKL
                     ADEAVCIGPAPSAQSYLNMPAIISTAEVTDAEAIHPGYGFLSENADFAERVEKSGFTF
                     IGPTPESIRMMGDKVSAKQAMIKAGVPCVPGSEGVLPEDPKEIIRIARTIGYPVIIKA
                     AGGGGGRGMRVVHTEAALVNAVQMTRSEAGAAFGNPNVYMEKFLEHPRHIEIQVLADT
                     HKNAVWLGERDCSMQRRHQKIIEEAPAPGIQRRVIEKIGERCAAACKRIGYRGAGTFE
                     FLYENGEFYFIEMNTRVQVEHPVTELVTGVDIVQMQIRVAAGEPLPFAQRQIQMRGHA
                     IECRVNAEDPYKFIPSPGRVTMWHPPGGPGVRVDSHIYTNYFVPSNYDSMVGKIIVHG
                     DTRDQALARMRTALSETVVEGIQTNIPLHRELMVDAKFIEGGTSIHYLEGWLAQHRR"
     gene            3136..4056
                     /gene="prmA"
                     /locus_tag="VF453_00915"
     CDS             3136..4056
                     /gene="prmA"
                     /locus_tag="VF453_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7686227.1"
                     /translation="MFELVLQAPEALVETVSDALMEELDALSVSVEDSDADTPAERAL
                     FGEPGMPPPSAGWARSTLKALFETEDAASAAATLLLAQDWRALFGDEAGPGPSVTALH
                     EVEEQDWVRLTQSQFDPVGITPGFWIVPTWHEPPAGARRVIRLDPGMAFGTGTHPTTR
                     MCLGWIAAHEAEVAGARVLDYGCGSGILAIGAAMHGAATVDAVDIDPAAVTTSQQNAQ
                     ANGVTLATGLPDLAAGEYAVVLANILATPLKMLAPLLCAHLAAGGHLVLAGILDRQAD
                     ELKAAYAPWLALEVARTEDGWILMTGRKAG"
     gene            4153..>4334
                     /locus_tag="VF453_00920"
     CDS             4153..>4334
                     /locus_tag="VF453_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX7686228.1"
                     /translation="MSLATRCPACGTIFRVVQDQLKVSEGWVRCGQCHEVFHGIEALF
                     DLDSDPAVAARRAATRT"
BASE COUNT          688 a         1529 c         1480 g          637 t
ORIGIN      
        1 cgcgatggga ttgggcgcgt ggctgcgctc gcgcggcgtg ccgctgtcgc cggtgcccga
       61 ggcgcaggcc ggcacgcctg gcccgggcgc gccggcgcag ggcgcgattc ccgtgccgct
      121 gacgacgctg gagggccgca cgctgaccgc cgcggacatc ggccaccgca gcgtcgtgct
      181 gaatttctgg gcgccgtggt gcccgccttg cgttcgcgag atgcccgaga tcgaccgctt
      241 ctcgcgctcg gcggcgggca aggacacgct cgtgatcggg ctggcgatcg acgagaagcc
      301 ggccgtcgac aagttcctcg ccgagcaccc ggtcggcttc ccgatcgtgg tcctcggcta
      361 ctcaggcctg tcgtgggtgc ggcagctcgg caatgacagc caggccctgc ccttcagcgt
      421 cgtcttcgac cgcggcgggc acaaggtcgt ccagcgcaag gccggggcga cgaccgccgg
      481 cgagctgcag ggctgggtgg cgaagctcta gtccggccgg gcagcggggg cgcttgccga
      541 ctcgatctcg cacgatccgc ccggatccgg gcgtgagatc ggcaaaatcc gggcccagac
      601 ccttagccgg ggtcggcgcg ggttgtcaag cactcttttt ggggtaaatt ctcgcttcgt
      661 tgcgtgatca gcacgtaacc ggcgtaaagt tcgcatcctt caaaacgaca cagtcggcgg
      721 ccccgcggcc gacttccgct tcgcatgaac gttctcgtgc ttcatggccc caacctgaac
      781 ctgctcggtc tgcgcgagcc ggcggtgtat ggaagtgcca cgttgcccga catagaacag
      841 caattgaagc agatcgctgc agaagccggc gcccggttgg agagcctgca gagcaaccat
      901 gaaggcgtgc tgatcgaccg cgtgcatgcg gcccgaaccg acggcaccga cttcatcgtg
      961 atcaatcccg cgggcttcac ccactcgagc gtcgcgctgc gcgacgcgct ggcggcggtc
     1021 gcgatcccgt tcgtcgaagt ccatctctcg aacatccatc gccgcgagcc gttccggcac
     1081 cactcgtatt tttccgacct ggccgaaggc gtcatctgcg gtctcggcgc gcagggatac
     1141 accctggcgc tgcggttcgc cctctcgcgc gcgcccgtgc gcgactgacg ccgatctccg
     1201 gccccaaggc gcccggccgg gcgctccctt gcccagacag acttttccca ggagaccgtt
     1261 ccatcatgga catgcgcaag ctcaagacct tggtcgacct cgtttcggag tccaacatct
     1321 ccgaactcga aatcacggaa gccgaaggca aggtccgcat cgtcaagggc ggctacggcg
     1381 gcgtgccggc gacgatgatg atgcagccga cggcgccgat gatgatgccg acccaggccc
     1441 aggcccagcc cgccgccgcg gccgagcccg ccgcgggtgc ggcgcccgag gcgcccgcga
     1501 tcaccggcca tgtggtcaag tcgccgatgg tcggcacgtt ctatcgctcc tccagccccg
     1561 gctcgaaagc cttcgtcgag atcggcagcc aggtcaagga aggcgacccg gtctgcatcg
     1621 tcgaggcgat gaagatcatg aacgagatcg aggccgacat ggccggcacc atcagcaaga
     1681 tcctggtcga gaacggccag gccgtcgagt acggccagcc gctgttcatc atcgagtaag
     1741 cggcccgcca cgtccgaccc ggatcctcgt ccatgttcaa gaaaatcctc attgccaacc
     1801 gcggcgagat cgcgctgcgc atccagcgcg cgtgccgcga gatgggcatc cagtccgtcg
     1861 tcgtctactc cgaggccgac cgcgacgcca agtacgtcaa gctcgccgac gaagcggtct
     1921 gcatcggccc ggcgccgtcg gcgcagagct acctcaacat gccggccatc atctcgaccg
     1981 ccgaggtgac cgacgcggag gcgatccacc cgggctacgg cttcctgtcc gagaacgccg
     2041 acttcgccga gcgcgtcgag aagagcggct tcaccttcat cggcccgacg cccgaatcga
     2101 tccgcatgat gggcgacaag gtgtcggcca agcaggcgat gatcaaggcg ggtgtgccct
     2161 gcgtgccggg ctccgagggc gtgctgccgg aggatccgaa ggagatcatc cgcatcgccc
     2221 gcacgatcgg ctacccggtc atcatcaagg ccgcgggcgg cggcggcggg cgcggcatgc
     2281 gcgtcgtgca cacggaagcg gccctcgtga acgcggtgca gatgacgcgc agcgaggccg
     2341 gtgcggcgtt cggcaacccg aacgtctaca tggagaagtt cctcgagcac cctcgccaca
     2401 tcgagatcca ggtgctcgcg gacacgcaca agaacgccgt ctggctgggc gagcgcgact
     2461 gctcgatgca gcgccgccac cagaagatca tcgaggaagc gccggcgccg ggcatccagc
     2521 gccgcgtgat cgagaagatc ggcgagcgct gcgccgccgc ctgcaagcgc atcggctacc
     2581 gcggcgcggg caccttcgag ttcctgtacg agaacggcga gttctatttc atcgagatga
     2641 acacgcgcgt gcaggtcgag cacccggtga ccgagctcgt cacgggcgtc gacatcgtgc
     2701 agatgcagat ccgcgtcgcc gccggcgagc cgctgccgtt cgcgcagcgg cagatccaga
     2761 tgcgcggcca cgccatcgag tgccgcgtca acgccgagga tccgtacaag ttcatcccgt
     2821 cgccgggccg cgtcacgatg tggcacccgc cgggcggccc cggcgtgcgc gtcgactcgc
     2881 acatctacac gaactacttc gtgccctcga actacgactc gatggtcggc aagatcatcg
     2941 tccacggcga cacgcgcgac caggcgctcg cgcgcatgcg cacggcgctg tcggagacgg
     3001 tggtcgaagg catccagacc aacatcccgc tgcaccgcga gctgatggtc gacgccaagt
     3061 tcatcgaggg cggcaccagc atccactacc tggaaggctg gctggcgcag catcgccgct
     3121 gatcggcccc gctccatgtt cgaactcgtc ctgcaggcgc ccgaggcgct ggtcgaaacg
     3181 gtctccgacg cgctgatgga ggagctcgac gcgctgtcgg tctccgtcga ggattcggac
     3241 gccgacacgc ccgccgagcg cgcgctgttc ggcgagccgg gcatgccgcc gccgagcgcc
     3301 ggctgggcgc gttcgacgct gaaggcgctg ttcgagaccg aggacgcggc cagcgccgcc
     3361 gcgacgctgc tgctcgcgca ggattggcgc gcgctgttcg gcgacgaagc cggcccgggt
     3421 ccgagcgtca cggcgctgca cgaggtcgag gagcaggact gggtgcggct cacgcagtcg
     3481 cagttcgacc cggtggggat cacgccgggc ttctggatcg tcccgacctg gcacgagccc
     3541 ccggccggcg cgcgacgcgt catccggctc gacccgggca tggccttcgg caccgggacg
     3601 catccgacga cccgcatgtg cctgggctgg atcgcggcgc acgaggccga ggtcgccggc
     3661 gcgcgcgtgc tggactacgg ctgcggctcg ggaatcctcg cgatcggcgc ggccatgcac
     3721 ggcgcggcga cggtcgacgc ggtcgacatc gacccggccg ccgtcaccac gtcgcagcag
     3781 aatgcgcagg ccaacggggt gacgctggcc accggcctgc cggacctggc cgccggcgag
     3841 tacgccgtcg tcctggccaa catcctcgcg acgccgctga agatgctcgc cccgctgctg
     3901 tgcgcgcacc tcgcggccgg cgggcacctg gtgctcgcgg gcatcctcga tcgccaggcc
     3961 gacgagctga aggccgccta cgcgccctgg ctcgcgctgg aggtcgctcg caccgaggac
     4021 ggctggatcc tcatgaccgg ccgcaaggcc ggctgatcgc cccgtcggcg tcggcccgga
     4081 tacggtggta gggtatgcaa gcccgtggcc ggccgcatcg gagcggctcc tgccgcgtgg
     4141 catgattcgc ggatgagcct cgccacccgc tgccccgcct gcggcacgat cttccgtgtc
     4201 gtgcaggacc agctcaaggt gtccgagggc tgggtgcggt gcggccagtg ccacgaggtg
     4261 ttccacggca tcgaggcgct gttcgacctc gacagcgatc cggccgtcgc ggcgcggcgc
     4321 gcggcgacgc gcac
//
LOCUS       DASVLN010000037         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_163405, whole genome shotgun sequence.
ACCESSION   DASVLN010000037 DASVLN010000000
VERSION     DASVLN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163405"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /gene="ybiB"
                     /locus_tag="VF453_00925"
     CDS             complement(<1..411)
                     /gene="ybiB"
                     /locus_tag="VF453_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HEX7686229.1"
                     /translation="MTIAPYLREIGRGKDGARALSREQAADLMARLLDGQVSDVEAGA
                     FAIAMRIKGETPAELAGFMDAVAPRLSPMPDPGAGGAVVLPSYNGARKLPNLVALLAL
                     ALARAGLPVLVHGLEEDPGRVTTAAVLAALGLPPV"
     gene            546..1706
                     /locus_tag="VF453_00930"
     CDS             546..1706
                     /locus_tag="VF453_00930"
                     /inference="COORDINATES: protein motif:HMM:NF042598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6765 family protein"
                     /protein_id="HEX7686230.1"
                     /translation="MQIDFHFGATYAIARIAGFAHAPAQTIATAAQYVDDSTTSGFLR
                     FDNGVRFHRLATAHPMISLENLSNDAAALSWLPFHFLPGNEGHDAPAPHGADAYRRRL
                     VCRPDSPVARAMMDDVIRRRDAPHALHRLGIAAHVFIDTFAHQGFVGERDPLNRARDL
                     QRADGRPLEGFEHLPALGHGQVGTYPDQPALRWRYVDGEDRVVERDNPRDFLRAADRL
                     CQEFRRHLAGDPAADAPGLGAALPAVRRLVEALVADSGEQRLEGWLEAIAGDAFGFGP
                     ALPRYVGKGEGSWKHLALGDDYLTWLQSAAAALHRREQELAGWRRIGAALAEDVVHRT
                     EALAVRLDLEEHSYRFSEGFLASDYKRFHDAAEEQRYIVFRTILPRFGIHSA"
     gene            1788..>2048
                     /locus_tag="VF453_00935"
     CDS             1788..>2048
                     /locus_tag="VF453_00935"
                     /inference="COORDINATES: protein motif:HMM:NF018389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEX7686231.1"
                     /translation="MTPFARRLLALSIVLAATSARAADPPAADVFKDWIVGCDNTRHC
                     EAVGYEAEDAAQDGVVALWIGRDAGPGAPVRARIGVGSVDQDD"
BASE COUNT          262 a          794 c          744 g          248 t
ORIGIN      
        1 caccggcggc aggcccagcg cggcgagcac ggccgccgtc gtgacgcggc cgggatcctc
       61 ctcgagcccg tgcacgagca ccggcagtcc cgcgcgggcg agcgccagcg ccagcagcgc
      121 gacgaggttg ggcagcttgc gcgcgccgtt gtacgacggc aggacgaccg cgccgcccgc
      181 gccggggtcg ggcatgggtg agagccgcgg cgcgacggcg tccatgaagc ctgcgagctc
      241 cgcgggcgtc tcgcccttga tgcgcatggc gatcgcgaag gcgccggcct cgacgtcgct
      301 cacctggccg tcgagcagcc gcgccatcag gtcggcggcc tgctcgcgcg agagggcgcg
      361 cgcgccgtcc ttgccgcggc cgatctcgcg cagataggga gcgatggtca tgggtgcagg
      421 gactttcggt gggaggaggc tcgggcgcaa tgccgcggcc attgtggcgc gcgtccggga
      481 aaccgctctc gcgccacggc ggcccggcgc gcacgatcgc gccggacgac cgggggagac
      541 cacgcatgca gatcgacttc cacttcggcg cgacgtacgc gatcgcacgc atcgccggct
      601 tcgcgcacgc gccggcgcag accatcgcca ccgcggcgca gtacgtcgac gactcgacga
      661 ccagcggctt cctgcgcttc gacaacggcg tgcgcttcca ccggctcgcg accgcgcacc
      721 cgatgatctc gctcgagaac ctgagcaacg atgccgcggc gctgagctgg ctgccgttcc
      781 acttcctgcc cggcaacgag ggccacgacg ccccggcccc gcacggcgcc gacgcctatc
      841 gccgccgcct ggtctgccgt cccgacagcc cggtggcgcg cgcgatgatg gacgacgtca
      901 tccgccgccg cgacgcgccg cacgcgctgc accggctggg catcgccgcg cacgtgttca
      961 tcgacacctt cgcgcaccag ggcttcgtcg gcgagcgcga tccgctgaac cgcgcccgcg
     1021 acctgcagcg cgccgacggg cgcccgctcg agggcttcga gcacctgccc gcgctcggcc
     1081 acggccaggt cggcacctat cccgaccagc cagcgctgcg ctggcgctac gtcgacggcg
     1141 aggaccgcgt ggtcgagcgc gacaacccgc gcgacttcct gcgcgccgcc gaccgcctgt
     1201 gccaggagtt ccggcgccat ctcgcgggcg atccggcggc cgacgcgccc ggcctcggcg
     1261 cggcgctgcc ggcggtgcgg cggctggtcg aggcgctcgt cgccgacagc ggcgagcagc
     1321 gcctggaggg ctggctggag gcgatcgccg gcgacgcctt cggcttcggc ccggcgctcc
     1381 cccgctacgt cggcaagggc gagggctcgt ggaagcacct ggcgctgggc gacgactacc
     1441 tgacctggct gcagagcgcc gccgcggcgc tgcatcggcg ggaacaggag ctcgcgggct
     1501 ggcgccgcat cggcgcggcg ctggccgagg acgtcgtgca ccgcaccgag gcgctggccg
     1561 tgcgcctgga cctcgaggag cattcgtacc ggttctcgga aggcttcctc gcgagcgact
     1621 acaagcgctt ccacgacgcc gccgaggagc agcgctacat cgtgttccgg acgatacttc
     1681 cgcgcttcgg catccactcg gcctgaccgc gccgaccgcc ccgcacgagc gccgaccgcc
     1741 ccgggggatg cgctcgcacc gcgggccgtc ggcccggagc ttcgcccatg acacccttcg
     1801 cacgccgcct gctcgcgctc tcgatcgtcc tcgccgccac gtccgcccgc gccgcggatc
     1861 cgcccgcggc cgacgtcttc aaggactgga tcgtcggctg cgacaacacg cgccactgcg
     1921 aggcggtcgg ctacgaggcc gaggacgcgg cccaggacgg cgtcgtcgcg ctctggatcg
     1981 ggcgcgacgc cggcccgggc gcgccggtgc gggcgcgcat cggcgtgggc tcggtcgacc
     2041 aggacgac
//
LOCUS       DASVLN010000038         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_109581, whole genome shotgun sequence.
ACCESSION   DASVLN010000038 DASVLN010000000
VERSION     DASVLN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109581"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..672
                     /locus_tag="VF453_00940"
     CDS             250..672
                     /locus_tag="VF453_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686232.1"
                     /translation="MRQFTKKLAIVGTAAGLMTALGVAWAQVATYADPNAAPDQSVTV
                     TNDHKPHIMYFADQGNSPETDPAAHLLLIKDAPQPQPVAMEETTTTSTTVADATPPAD
                     TSSQSTTSTDTTAAAPVDTTSTAPADDTSAPPPKADRN"
     gene            complement(789..1595)
                     /locus_tag="VF453_00945"
     CDS             complement(789..1595)
                     /locus_tag="VF453_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7686233.1"
                     /translation="MDRIPTPRWTAVLADDEPWLRADLREQLAACWPELEVVGEAADG
                     VEAVQLVQRLACDVVFLDVQMPRMTGVDAAARLKGQVEIVFLTAYDQHAVAAFEHRAA
                     DYLLKPLAADRLADTVARLRARLADRHDGQAVAQRLAALERALVEQAPGQAAANVPAA
                     ERLEWIKAVVGNATRLIAVDEVLALRAVPGYTQVVTAETEALIRTPLRDLLPGLDPRR
                     FVQVHRNAIVNLRAVAVARRLADGRYAIELRHGRGTIETSRSRAGVFREE"
     gene            complement(1598..>2748)
                     /locus_tag="VF453_00950"
     CDS             complement(1598..>2748)
                     /locus_tag="VF453_00950"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7686234.1"
                     /translation="AAVEPPPASTRPDVPADDDADAESNSLRQHAPIVAVLTTGLSAL
                     IALEGPREWLVFLHLLLPVLALTLAGTFAAALGVPGRRAAQWRGYPLRCAFGGVCGGL
                     VGIWISVAWRGRSLERFAERPIAWHMVAGSLVTGALVGIALWWLEHLRRRERRREREL
                     FSVQAELLRSRTDAAEAEAARIRMSLQLLQAQVEPHFLYNALANLRYLVRHDAVLAQK
                     LVDHLVRYFRVALPSLRELEVTLAQELELCSAFAAIQSMRLGDRLRVEIDVAPEWHDA
                     RLPPALLLTLLENAFKHGMPADGAPAHVRVEARGCGGILTLSVTDNGPGPVRRPGEVT
                     PANGSGTGIKWLGERLRAVFGDRACLSLEQAPGGGCRAVLLMPVKRSD"
BASE COUNT          381 a         1057 c          986 g          324 t
ORIGIN      
        1 gcggcgatct cgcgccagct cggctggaac gccgcgccgc cgctgccggg cggcggcgcc
       61 tgaggaagcc tggcgcgcgg cgaccgtctt gccgccggcg caggccgaac tggacggtgc
      121 gcgcggacgc tgacgtcgac ctgaggcgcg gctgcgcggg caccgtgatt gctgtgtccg
      181 ggttactgcg gatggacgcg ctgcgcgttc cgcatggcat tcctcccaac ccacacagga
      241 gctcgcatca tgcgtcaatt caccaagaag ctcgcgatcg tcggtaccgc cgccggcctc
      301 atgacggccc tgggcgtcgc ctgggcccag gtcgccacct atgccgaccc gaacgccgcg
      361 cccgaccaga gcgtgacggt cacgaacgac cacaagccgc acatcatgta tttcgccgac
      421 cagggcaact cgcccgagac ggatcccgcg gcccacctgc tgctgatcaa ggacgcgccg
      481 cagccgcagc cggtcgccat ggaagagacg accacgacct cgacgacggt cgccgacgcc
      541 acgccgccgg ccgacacctc gtcgcagtcg acgacctcga ccgacacgac cgccgcggcc
      601 ccggtcgaca cgacgagcac cgcgccggcc gacgacacct cggcgccccc gccgaaggcc
      661 gaccgcaact gacggcctga cgcacctgcc acgcgcgcga cgggctcgcc tgtcgcgcgc
      721 gcgctcgcga ggcaagcgcc gtcctgcgga ctgcggtgcg agctcccttc ggaacggccg
      781 gcgggggttc attcctcccg gaacacgccg gcgcggctgc ggctcgtctc gatcgtcccg
      841 cgcccgtggc gcagctcgat cgcgtaacgg ccgtcggcca ggcgccgcgc gaccgcgacc
      901 gcgcgcaggt tgacgatcgc gttgcggtgc acctgcacga agcggcgcgg gtcgaggccg
      961 ggcagcaggt cgcgcagggg cgtgcggatg agcgcttccg tctccgcggt gacgacctgc
     1021 gtgtagcccg gcacggcgcg cagcgccagc acctcgtcca ccgcgatcag ccgggtggcg
     1081 ttgccgacca cggccttgat ccattcgagc cgctctgccg cggggacgtt cgccgcggcc
     1141 tgtccgggcg cctgttcgac gagcgcccgc tccagcgccg cgagccgctg cgcgaccgcc
     1201 tggccgtcat gccgatcggc gagccgcgcg cgcagccgcg ccaccgtgtc ggcaagacgg
     1261 tctgcagcga gcggcttgag caggtagtcg gccgcccggt gctcgaacgc ggcgaccgcg
     1321 tgctggtcgt acgcggtcag gaacacgatc tcgacctggc ccttgagccg ggccgcggcg
     1381 tccacgcccg tcatgcgcgg catctgcacg tcgaggaaga cgacgtcgca cgcgagccgc
     1441 tgcacgagct gcaccgcctc gacgccgtcc gccgcctcgc ccacgacctc gagctccggc
     1501 cagcacgcgg cgagctgctc gcgcaggtcg gcgcgcagcc agggctcgtc gtcggcgagg
     1561 acggccgtcc agcgcggcgt ggggattcgg tccatcgtca atcgctccgc ttgaccggca
     1621 tcagcagcac cgcacggcag ccgccgccgg gcgcctgttc gaggctcagg caggcgcggt
     1681 cgccgaacac cgcgcgcagt cgctcgccga gccacttgat gcccgtgccc gagccgttcg
     1741 ccggcgtcac ctcgccgggc cggcgcacgg ggccggggcc gttgtcggtg acgctcagcg
     1801 tgaggatgcc gccgcagccg cgcgcctcga cgcgcacgtg cgcgggcgcg ccgtcggccg
     1861 gcatgccgtg cttgaacgcg ttctccagca aggtcagcag cagcgcgggc ggcaggcggg
     1921 cgtcgtgcca ttcgggggcg acgtcgatct ccacccgcag ccggtcgccc aggcgcatcg
     1981 actggatcgc cgcgaaggcg gagcacagct cgagctcctg ggcaagcgtc acctcgagct
     2041 cgcgcaggct cggcagcgcg acgcggaagt agcgcacgag gtggtcgacg agcttctgcg
     2101 ccagcaccgc gtcgtggcgc accaggtagc gcaggttggc gagcgcgttg tagaggaagt
     2161 gcggctcgac ctgcgcctgc agcagctgca gcgacatgcg gatgcgcgcg gcctcggcct
     2221 cggcggcgtc ggtgcgcgag cgcagcagct ccgcctggac ggagaacagc tcgcgctcgc
     2281 gccggcgctc gcggcgtcgc aggtgctcca gccaccacag cgcgatgccg accagcgcgc
     2341 ccgtcacgag gctgccggcg accatgtgcc aggcgatcgg gcgctccgcg aagcgctcca
     2401 gcgagcgtcc gcgccacgcc acgctgatcc agatccccac caggccgccg cagacgccgc
     2461 cgaacgcgca tcgcagcgga tagccgcgcc actgcgccgc gcggcggccc ggcacgccca
     2521 gcgccgcggc gaacgtgccg gcgagcgtca gcgccagcac cggcagcagc aggtgcagga
     2581 acacgagcca ctcgcgcggc ccctcgagcg cgatgagcgc gctcagcccg gtcgtgagga
     2641 cggccacgat cggcgcgtgc tggcgcaggc tgttcgactc ggcgtccgca tcgtcgtccg
     2701 cgggcacgtc cggccgcgtc gaggcgggcg ggggctccac cgccgcgg
//
LOCUS       DASVLN010000039         8497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_109596, whole genome shotgun sequence.
ACCESSION   DASVLN010000039 DASVLN010000000
VERSION     DASVLN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109596"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VF453_00955"
     CDS             complement(<1..894)
                     /locus_tag="VF453_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686235.1"
                     /translation="MRSTQMSRLLERLDAEIAQAEGVYERECLKSRRAALLARHGQFA
                     EARFALQGLRSQSQRLKNPALSAWVALVEGLIEHFGALAPSSRAKFRRAHELASIAGE
                     PGLLAECAAWLSNADLNANDPEATCAHASEAVRVAPADGHAALARATLVIGNCWRVAG
                     HDARAQPWYKRARQHAASEGDVSLVSVMLHNLAAFQAGRIGLEDAFGRADVADAQRVL
                     LEAESTGNYDAGVGNDMLLAEMPLVRAQLLTVLGRHADAIALIDAELPRARHEGQGAR
                     EARLLADALYGEARLGRADEAA"
     gene            complement(921..1820)
                     /locus_tag="VF453_00960"
     CDS             complement(921..1820)
                     /locus_tag="VF453_00960"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX7686236.1"
                     /translation="MPADALLTTSLHSLPLLARGKVRDNYAVGEDRILMVATDRLSAF
                     DVVLGEPIPGKGALLTQMALFWFGKLEGIVPNHLTGEAPESVVQPDEVAQVRDRSMLV
                     KRLKPLPIEAVVRGYLAGSGWKEYQESQSVCGVKLPAGLKNASKLPQPIFTPATKAEA
                     GAHDENISFDVAASMIGAELAARVRDVSIALYERAAEYALTKGIIIADTKFEFGLDAD
                     GTLTLMDEVLTPDSSRYWPVDGYAEGHNPASYDKQFVRDWLESVRIDGQPWNKKAPAP
                     AMPADVAERTAAKYREAFQRLAG"
     gene            complement(1987..3051)
                     /gene="fba"
                     /locus_tag="VF453_00965"
     CDS             complement(1987..3051)
                     /gene="fba"
                     /locus_tag="VF453_00965"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903026.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX7686237.1"
                     /translation="MPLVSMRQLLDHAAENGYGIPAFNVNNLEQVQAVMTAAAEVGAP
                     VILQASAGARKYAGEPFIKHLIQAAVESWPQIPLVMHQDHGQSPDVCRGAIDLGFSSV
                     MMDGSLQADGKTIASYDYNVDVTRKVVDMAHKLGVTVEGELGCLGSLETMKGDKEDGH
                     GTDATMTMEQLLTDPEQAADFVKKTQLDALAIAIGTSHGAYKFTRKPTGDILAISRIK
                     EIHRRIPNTHLVMHGSSSVPQELLAIIREHGGAMKETYGVPVEEIQEAIKHGVRKINI
                     DTDIRLAMTGAIRKYMAENPDKFDPRDYLKPAREAAKQICRQRYVEFGCEGQAGKIKP
                     ESLQVVAARYESGQLAQTVN"
     gene            3444..4457
                     /locus_tag="VF453_00970"
     CDS             3444..4457
                     /locus_tag="VF453_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7686238.1"
                     /translation="MARIELSTLTRTITADALAHPTDLGPMLARRHSLSRGTMGKHLR
                     RLVESGWLVRTGGSRPCFAPGRQREVTHEYAVAGLDEHTPWLRDIHPVLALPPAVARV
                     AQHVFGELLNNAIDHAQATRVTVSVRQTPTHLHILIGDDGLGVFNQLAQKAQLPDAQR
                     AALEISKGRLTTQPECHGGRGLFFVARMVDAMNLQSNGQVLQWRHRGESVQFRTHPLN
                     RTGTTVFVSLALDTQVRPTDAYSAYGSDDAPLDFSRTQVPLRLLTDNGLTLDSRAQAR
                     WVVSRLELFATAELDFDGVDDVGPSFIDEVFRVYANAHPQVKLQPTKTSERVGKLLAA
                     SVH"
     gene            complement(4720..6150)
                     /gene="pyk"
                     /locus_tag="VF453_00975"
     CDS             complement(4720..6150)
                     /gene="pyk"
                     /locus_tag="VF453_00975"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX7686239.1"
                     /translation="MTRGTKIVATLGPATTDAQVLERLIRAGVDVVRLNFSHGKAQDH
                     IDRANLVREIAERVGRTVALMADLQGPKIRVGKFAEGKVMLENGARFVLDASRMEPGD
                     VEGVGLDYKELPRDVKPGDTLLLNDGLIVLTVDEVRGDQVHTTVRIGGELSNNKGINK
                     QGGGLTAPALTAKDMDDIRTAMSFRCDYLAVSFPKNATDMEMARQLANVAGESQRHKP
                     GMIAKIERTEAIPNLEAILRASDGIMVARGDLAIEVGNAAVPALQKRMIRMARDMDKV
                     VITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETAAGRYPVETVETMSAI
                     VVEAERAEDVSLDTDFVNKKFGRIDQSIAMGALFTAHHLGCKAIVALTESGSTALWMS
                     RHRTHVPVFALTSQPLSQRRMALYRNVQPLLIQAFDDRDQALAAAEKLLVAQGVLRPG
                     DTYAITCGEPMGYPGGTNMLKVCRVN"
     gene            complement(6389..7582)
                     /locus_tag="VF453_00980"
     CDS             complement(6389..7582)
                     /locus_tag="VF453_00980"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7686240.1"
                     /translation="MNVIRFSDLVAAGKVTAKRVFIRADLNVPQDDAGNITEDTRVRA
                     SVPCIRMALDAGAAVMVTSHLGRPTEGEFKPEDSLAPVARRLGELLGRDVPLVANWVD
                     GVDVKPGSVVLLENCRVNKGEKKNNPELAKKMAALCDIFVNDAFGTAHRAEGTTYGIA
                     EYAPVACAGPLLAAEIDAITQALANPKRPLVAIVAGSKVSTKLTILQSLSEKVDGLIV
                     GGGIANTFMLAAGLKIGKSLAEPDLVGEAKAVIAKMQARGAAVPIPTDVVVARKFAAD
                     AEATVKAAADVADDDLILDIGPQTAAKLAEQLKAAGTIVWNGPVGVFEFDAFAHGTET
                     LARAIAASPAFSIAGGGDTLAAIAKYGIDKDVGYISTGGGAFLEVLEGKTLPAFEILE
                     KRAAG"
     gene            complement(7824..8288)
                     /locus_tag="VF453_00985"
     CDS             complement(7824..8288)
                     /locus_tag="VF453_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686241.1"
                     /translation="MSRPLLALLAAAVLVPLAGCDQLGIESATVTAARVEADGKAVGA
                     ACRHGGRAIEDCFALNKRVDKAAIYAGWRDMDDYMRENKIEVVPPTLGPGAKVADKSS
                     DGDDASPADDADKADKADKADKAAPKAADKPRAGDKAADRAGADAGKSAAHG"
BASE COUNT         1105 a         3031 c         3053 g         1308 t
ORIGIN      
        1 ggccgcctcg tccgcgcgcc cgagccgggc ctcgccgtag agcgcgtcgg ccagcaggcg
       61 cgcctcgcgg gcgccctggc cctcgtggcg ggcgcgcggg agctccgcgt cgatcagcgc
      121 gatcgcgtcg gcgtggcggc ccagcaccgt cagcagctgc gcgcgcacga gcggcatctc
      181 ggcgagcagc atgtcgttgc ccacgcccgc gtcgtagttg ccggtggact cggcctccag
      241 cagcacgcgc tgcgcatcgg cgacgtcggc gcggccgaag gcgtcctcca gcccgatgcg
      301 gccggcctgg aacgcggcga ggttgtgcag catgacgctc acgagcgaga cgtcgccctc
      361 cgaggccgcg tgctggcggg cgcgcttgta ccagggctgc gcgcgggcat cgtggccggc
      421 gacgcgccag cagtttccga tcacgagcgt cgcgcgcgcg agcgcggcgt ggccgtcggc
      481 gggcgcgacg cgcacggcct cgctcgcgtg ggcgcaggtc gcctcggggt cgttggcgtt
      541 gaggtcggcg ttcgacagcc aggccgcgca ctcggccagc aggccgggct cgcctgcgat
      601 cgaggcgagc tcgtgcgcgc gccggaactt cgcccgcgac gacggcgcca gcgcgccgaa
      661 gtgctcgatc aatccctcga ccagcgcgac ccacgcactc agcgccgggt tcttcaggcg
      721 ctggctctgc gagcgcaggc cctgcagcgc gaagcgcgcc tccgcgaact ggccgtggcg
      781 cgcgagcagc gccgcgcggc gcgacttcag gcactcgcgc tcgtacacgc cttccgcctg
      841 ggcgatctcg gcgtccaggc gttcgagcag gcggctcatc tgcgtgctgc gcaagggacg
      901 acgggcgctc gggggagtcg tcagccggcc aggcgctgga acgcctcgcg gtacttggcg
      961 gccgtgcgct cggcgacgtc cgcgggcatc gccggggccg gcgccttctt gttccagggc
     1021 tggccgtcga tgcgcacgct ctcgagccag tcgcgcacga actgcttgtc gtagctggcc
     1081 gggttgtgcc cttcggcgta gccgtcgacc ggccagtagc gcgacgaatc gggcgtcagc
     1141 acctcgtcca tcagcgtgag cgtgccgtcg gcatcgaggc cgaactcgaa cttggtgtcg
     1201 gcaatgatga tgcccttggt cagcgcgtac tcggcggcgc gctcgtacag cgcgatcgag
     1261 acgtcgcgca cccgggccgc gagctcggcg ccgatcatcg aggcggcgac gtcgaaggag
     1321 atgttctcgt cgtgcgcgcc ggcctcggcc ttggtggcgg gcgtgaagat gggctggggc
     1381 agcttcgagg cgttcttcag gccggcgggc agcttcacgc cgcagaccga ctggctctcc
     1441 tggtactcct tccagcccga gccggcgagg tagccgcgca ccaccgcctc gatcggcagg
     1501 ggcttgaggc gcttgaccag catcgagcga tcgcgcacct gcgccacctc gtcgggctgg
     1561 acgaccgact ccggcgcctc gccggtcagg tgattgggga cgatgccctc gagcttgccg
     1621 aaccagaaca gggccatctg cgtgagcagc gcgcccttgc ccgggatcgg ctcgccgagc
     1681 acgacgtcga acgccgagag ccgatcggtc gcgaccatca ggatgcggtc ctcgccgacg
     1741 gcgtagttgt cgcgcacctt gccgcgcgcc agcagcggca gggagtggag ggacgtggtc
     1801 agcagcgcgt cggcgggcat ggcagaggca gttctttgga aagcccttat tgtgccttgt
     1861 caccccggct ccggcgccgc cgctcgtgca tcgcgaacga gacgccgggg agcgacggat
     1921 ccggctgcgc cgggccgctc gcggccagcc cctcgggggc agggagcgcc agcgaccgcg
     1981 gggtcgtcaa ttcacggtct gggcgagctg gccgctctcg tacctggccg cgacgacctg
     2041 aagcgactcc ggcttgatct tgccggcctg cccctcgcag ccgaactcga cgtagcgctg
     2101 gcggcagatc tgcttggccg cctcgcgcgc gggcttcagg tagtcgcgcg ggtcgaactt
     2161 gtccgggttc tcggccatgt acttgcggat cgcgccggtc atcgcgaggc ggatgtcggt
     2221 gtcgatgttg atcttgcgca cgccgtgctt gatcgcctcc tggatctcct cgaccggcac
     2281 gccgtaggtc tccttcatcg cgccgccgtg ctcgcggatg atcgccagca gctcctgcgg
     2341 caccgacgac gagccgtgca tcacgaggtg ggtgttcggg atcctgcggt ggatctcctt
     2401 gatgcggctg atcgcgagga tgtcgccggt gggcttgcgc gtgaacttgt acgcgccgtg
     2461 gctcgtgccg atcgcgatcg ccagcgcgtc cagctgcgtc ttcttgacga agtcggcggc
     2521 ctgctcggga tcggtcagca actgctccat cgtcatcgtg gcgtcggtgc cgtggccgtc
     2581 ctccttgtcg cccttcatcg tctcgagcga gccgaggcag cccagttcgc cctcgaccgt
     2641 cacgcccagc ttgtgggcca tgtcgacgac cttgcgcgtg acgtcgacgt tgtagtcgta
     2701 gctggcgatg gtcttgccgt cggcctgcag cgagccgtcc atcatcaccg agctgaagcc
     2761 gaggtcgatc gcgccgcggc agacgtccgg gctctggccg tgatcctggt gcatgaccag
     2821 cgggatctgc ggccagctct ccaccgccgc ctggatcagg tgcttgatga agggctcgcc
     2881 ggcgtacttg cgggcgccgg ccgaggcctg caggatgacc ggggcgccga cttcggccgc
     2941 cgcggtcatc accgcctgca cctgctcgag gttgttgacg ttgaacgccg ggatgccgta
     3001 gccgttctcg gccgcgtgat ccagcagttg acgcatcgaa acgagaggca tgggaagtcc
     3061 ttcagggatg ttgcgaaagc gggtcgcggc acggcggcgc accaccgtgc cacagaatcg
     3121 ggcggcgcgg ttcgtaacct cgccgtaact tgcccatttt atcgatgtgg gcgggcgccg
     3181 gaggcaatcg ccttgcccgg ccgtccgagg acgcgcccgc gaggcgccgg ccagccaacc
     3241 gtcgccgcgg ggcttgccgg catgcggggg gcaccgatta cgccgcggtt acggcaccgc
     3301 gggcaccccg gccggcagcc gcgctcgcgc cggcggccgg cgaattcccg ggtgtttccg
     3361 gccctgttcc acggctccca gccctcacgt tccgggacac ttcgtccgac acggccgccg
     3421 ccgcggccat cccggagccc tgcatggcac gcatcgaact ctccacgctg acccgcacga
     3481 tcaccgccga cgcgctcgcg catccgaccg acctcggccc gatgctggcg cgccggcact
     3541 cgctgtcgcg cggcacgatg ggcaagcacc tgcgccggct cgtggaaagc ggctggctgg
     3601 tgcgcaccgg cggctcgcgc ccctgcttcg cgcccggccg ccagcgcgag gtcacccacg
     3661 aatacgccgt cgccggcctc gacgagcaca ccccctggct gcgcgacatc cacccggtgc
     3721 tcgcgttgcc gcccgcggtc gcgcgcgtcg cgcagcacgt cttcggggag ctcctgaaca
     3781 acgcgatcga ccatgcgcag gcgacgcgcg tcacggtgtc ggtgcgccag acgccgacgc
     3841 acctgcacat cctgatcggc gacgacggcc tgggcgtgtt caaccagctc gcgcagaagg
     3901 cgcagctccc cgacgcgcag cgcgcggcac tcgagatctc caagggccgc ctgacgacgc
     3961 agcccgagtg ccacggcggc cgcggcctgt tcttcgtcgc gcgcatggtc gacgcgatga
     4021 acctgcagtc caacggccag gtgctgcagt ggcggcaccg cggcgagtcg gtgcagttcc
     4081 gcacccatcc gctcaaccgc accggcacca ccgtgttcgt gagcctggcg ctcgacaccc
     4141 aggtgcgccc gaccgacgcc tacagcgcct acggctccga cgacgccccg ctcgacttca
     4201 gccgcaccca ggtgccgctg cggctgctga ccgacaacgg cctcacgctc gactcccgcg
     4261 cccaggcgcg ctgggtcgtc tcgcggctgg agctgttcgc gaccgcggag ctcgacttcg
     4321 acggcgtcga cgacgtcggc cccagcttca tcgacgaggt cttccgcgtc tatgcgaacg
     4381 cccacccgca ggtgaagctg caaccgacca agaccagcga acgcgtcggc aagctgctcg
     4441 cagcgtcggt gcactgaacc aaggcgcaag cgccacgacg cgcccccccg acaagcgcga
     4501 gcgcagcaac gcccccccac agcgcgagcg cagcgacgcg ccccccagcg cgagcgcagc
     4561 gacgcgcccc cagcgcgagc gcagcgacgc gccgcccagc gccggcgctg ccgctcgaac
     4621 ggcccaaacg agacacgggg aagcggcaga tccggctccg ccggtctgca cgcgcaagcc
     4681 ccctcggggg gcagcccgcg ccagcgggcg tgggggccgt caattcactc tgcagacctt
     4741 gagcatgttg gtgccgccgg gatagcccat cggctcgccg cacgtgatcg cgtaggtgtc
     4801 tcccggacgc agcacgccct gggcgacgag cagcttctcg gccgccgcca gcgcctggtc
     4861 gcggtcgtcg aaggcctgga tcagcagcgg ctgcacgttg cggtacagcg ccatgcggcg
     4921 ctgcgacagc ggctgggagg tcagcgcgaa gaccggcacg tgggtgcggt ggcgactcat
     4981 ccagagcgcg gtcgagccgg actcggtcaa cgcgacgatc gccttgcagc ccaggtggtg
     5041 cgccgtgaag agcgcgccca tcgcgatcga ctggtcgatg cggccgaact tcttgttgac
     5101 gaagtccgtg tccagcgaga cgtcctcggc acgctcggcc tcgacgacga tggccgacat
     5161 ggtctccacc gtctcgaccg gatagcgccc tgcggcggtc tcggcgctga gcatcacggc
     5221 gtcggtgccg tccagcaccg cgttggcgac gtccgagacc tcggcgcgcg tcggcaccgg
     5281 cgcgtggatc atgctctcca tcatctgcgt ggcggtgatg acgaccttgt ccatgtcgcg
     5341 cgccatgcgg atcatgcgct tctgcagcgc cggcaccgcg gcgttgccga cctcgatggc
     5401 caggtcgccg cgcgcgacca tgatgccgtc gctcgcgcgc aggatggcct cgaggttggg
     5461 gatcgcctcg gtgcgctcga tcttggcgat catgcccggc ttgtgacgct gcgactcgcc
     5521 cgccacgttg gcgagctggc gcgccatctc catgtcggtc gcgttcttcg ggaagctgac
     5581 tgcaaggtag tcgcagcgga agctcatggc cgtgcggatg tcgtccatgt ccttggcggt
     5641 gagcgccggc gcggtcaagc cgccgccctg cttgttgatg cccttgttgt tggacagctc
     5701 gccgccgatg cgcaccgtgg tgtgcacctg gtcgccgcgc acctcgtcga ccgtcagcac
     5761 gatcaggccg tcgttcagca gcagcgtgtc gcccggcttc acgtcgcgcg gcagctcctt
     5821 gtagtcgagg ccgacgccct cgacgtcgcc cggctccatg cgcgaggcgt cgagcacgaa
     5881 cctcgcgccg ttctcgagca tcaccttgcc ttcggcgaac ttgccgacgc ggatcttcgg
     5941 cccctgcagg tcggccatca gcgcgacggt gcggccgacg cgctcggcga tctcgcgcac
     6001 gaggttggcg cggtcgatgt ggtcctgcgc cttgccgtgg ctgaagttca gccgcaccac
     6061 gtcgacgccg gcccggatga ggcgttccag cacctgtgcg tcggtggtgg cggggccgag
     6121 cgtggcgacg atcttggtac cgcgggtcat gagggactgt ctcctggagg gcgctggtgg
     6181 aatggacgcg aaattatggg cgacgcgggc gccgccgggt tacctcgacg ttacggaatc
     6241 acgccttggc gagcacggcg gcgacgcaag cggcagagtg tgcggtgaaa cccggtttcg
     6301 cgccagcggg cgccgtgatt cgcgtcgcga aggcattccc gcgccgcggc cggggacgtt
     6361 ccgcggccgc ggacgcggcg ccggcgcgtc agccggccgc gcgcttctcg aggatctcga
     6421 acgccggcag cgtcttgccc tccagcacct cgaggaaggc gccgccgccg gtcgagatgt
     6481 agccgacgtc cttgtcgatg ccgtacttgg cgatcgcggc gagggtgtcg ccgccgccgg
     6541 cgatgctgaa ggccgggctc gcggcgatcg cgcgcgccag cgtctcggtg ccgtgcgcga
     6601 aggcgtcgaa ctcgaacacg ccgaccgggc cgttccagac gatggtgccc gcggccttga
     6661 gctgctcggc gagcttcgcg gcggtctgcg ggccgatgtc gaggatcagg tcgtcgtcgg
     6721 cgacgtcggc tgccgccttg acggtggcct cggcgtcggc ggcgaatttc ctggcgacga
     6781 ccacgtcggt cgggatcggc accgccgcgc cgcgcgcctg catcttggcg atcacggcct
     6841 tggcctcgcc cacgaggtcc ggctcggcca ggctcttgcc gatcttcagg cccgcggcca
     6901 gcatgaaggt gttggcgatg ccgccgccga cgatcagccc gtcgaccttc tccgacagcg
     6961 actgcaggat ggtcagcttg gtcgagacct tgctgcccgc gacgatggcc accagcggcc
     7021 gcttcgggtt cgcgagcgcc tgcgtgatgg catcgatctc ggcggccagc agcgggccgg
     7081 cgcaggcgac gggcgcgtac tcggcgatgc cgtaggtcgt gccctcggcg cggtgggcgg
     7141 tgccgaaagc gtcgttgacg aagatgtcgc agagcgcggc catcttcttc gcgagctcgg
     7201 ggttgttctt cttctcgccc ttgttgacgc ggcagttctc cagcagcacg acgctgcccg
     7261 gcttcacgtc cacgccgtcg acccagttcg cgaccagcgg cacgtcgcgg cccaggagct
     7321 cgcccaggcg cctggccacg ggcgcgagcg agtcctcggg cttgaactcg ccctcggtcg
     7381 ggcggccgag gtggctcgtg accatcacgg cggcgccggc gtccagcgcc atgcggatgc
     7441 acggcaccga ggcgcgcacg cgggtgtcct cggtgatgtt gccggcgtcg tcctgcggca
     7501 cgttgaggtc ggcgcggatg aagacgcgct tggccgtcac cttgccggcg gcgacgaggt
     7561 cggagaaacg gatcacgttc atgatggctg tctcgagcgg tggagttgtc gggagaggtc
     7621 gtggcggcgc gcggggccgc cggagccgca agtatcctcg cccggaggtc cggcgcgagt
     7681 acgaatgcgc gccgcgcggg cgcgaccggg cccgcgccgc attgtcgtcc tcgggagcgg
     7741 acacaggggc gcgacggggc gccccgcgag cgcgaggcgc cgttcttcgg atggcgggag
     7801 cgtccggcga cgccggaccg cggtcagccg tgggcggcgg acttgccggc gtcggcgcca
     7861 gccctgtcgg cggccttgtc gcccgccctg ggcttgtcgg cggccttggg cgccgccttg
     7921 tcggccttgt ccgccttgtc ggccttgtcc gcgtcgtcgg caggcgacgc gtcgtcgccg
     7981 tcggaggact tgtccgcgac cttggcgccc gggccgagcg tcggcgggac gacctcgatc
     8041 ttgttctcgc gcatgtagtc gtccatgtcg cgccagccgg cgtagatggc ggccttgtcg
     8101 acccgcttgt tgagcgcgaa gcagtcctcg atcgcccgcc cgccgtggcg gcaggcggcg
     8161 ccgacggcct tgccgtccgc ctcgacgcgc gcggcggtca cggtggccga ctcgatgccg
     8221 agctggtcgc aaccggcaag ggggacgagg acggcggcgg cgaggagggc gagcaggggg
     8281 cgcgacatgg cgatttcggg gctgtgcgac cctatatcgg ccgatcggcg catcgcttga
     8341 gccgccgatc gccgcgaccg ggccgcgaac gccggccagt tcgtacgcgg tcagcccagg
     8401 acgtcgccga ccgccgccgg ccgcgactgc agccaggccg ccaccggctg gcgcctgccg
     8461 cccgggcgct gcacctcgac gagggcgagc gcgtccg
//
LOCUS       DASVLN010000040         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_55701, whole genome shotgun sequence.
ACCESSION   DASVLN010000040 DASVLN010000000
VERSION     DASVLN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55701"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1635)
                     /locus_tag="VF453_00990"
     CDS             complement(<1..>1635)
                     /locus_tag="VF453_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX7686242.1"
                     /translation="PEGARLLRASEQAALRFGQRFGLLDAAAATTIERASFALLDYLR
                     RQLAGKPVSPLALFPQYCEVQVLAGADRIHPADLWQAEGAWQGAALAANEQALGQSAL
                     SGAEPLRADADTRGRMEQCMLALLRGPVGPAATQMSRLFAGLAAGSADRGTAKLAGLW
                     AIAAGFFEAQAQGRLDADVYAKRLTSRLLAQVRLAERGQSEVSDRLVQDLLFFCARAR
                     SDAATTPRLAAVRQAFRLPQAEAVDYEQEALGRYDPALIPLARRRVEALKESWSSATA
                     GQSGLAASLREQATAVADVVRKLHPEGEHMARALDAAVTQVADGNIVPTPGLAMEVAT
                     GLLCLDAALDDADIADAAFGTRIQQLAERIDLARQGRTPGPVEPWMEELYRRASDRQT
                     MGSVVQELRSQLAEVELQLDKFNRAPGDREPLVPVPAKLSAMRGVLSVLGLDQAGQAV
                     VHMRDEVDAVLARPAGEVDEAAARALSEQSVGRLAANVGALGFLIDLLGVQPLLAKAL
                     FRFDADAGRLEAVMGRTEAAAPAANEAAPAPVSATP"
BASE COUNT          199 a          600 c          650 g          186 t
ORIGIN      
        1 gcggggtcgc ggagaccggt gccggcgccg cctcgttggc ggccggggcg gcggcctcgg
       61 tgcggcccat cacggcctcg aggcggcccg cgtcggcatc gaagcggaac agcgccttgg
      121 cgagcagcgg ctgcacgccc agcaggtcga tcagaaagcc gagcgcgccg acgttggcgg
      181 ccaggcggcc gacggactgc tccgacagcg cacgcgcggc cgcctcgtcc acctcgcccg
      241 cggggcgcgc gagcacggcg tcgacctcgt cgcgcatgtg gacgacggcc tggcccgcct
      301 ggtcgaggcc gagcaccgag agcacgccgc gcatcgcgga cagcttggcc ggcacgggca
      361 ccagcggctc gcggtcgccg ggcgcgcggt tgaacttgtc cagctgcagc tcgacctcgg
      421 ccagctgcga gcgcagctcc tgcacgacgc tgcccatggt ctggcgatcg gaggcgcggc
      481 ggtacagctc ctccatccaa ggctcgaccg ggccgggcgt gcggccctgg cgcgcgaggt
      541 cgatgcgctc ggcgagctgc tggatgcggg tgccgaaggc ggcgtcggcg atgtcggcgt
      601 cgtccagggc ggcgtccagg cacagcaggc cggtggcgac ctccatcgcg aggcccggcg
      661 tcggcacgat gttgccgtcg gcgacctgcg tcaccgcggc gtcgagcgcg cgcgccatgt
      721 gctcgccctc ggggtgcagc ttgcgcacga cgtcggcgac ggccgtcgcc tgctcgcgca
      781 ggctggcggc gaggccggac tggccggcgg tggccgagga ccacgactcc ttgagcgctt
      841 ccactcgccg gcgcgcgagc gggatcaacg cggggtcgta ccggcccagg gcctcctgct
      901 cgtagtcgac cgcctcggcc tgcggcaggc ggaaggcctg gcgcacggcg gccaggcgtg
      961 gcgtcgtcgc ggcgtcgctg cgggcgcggg cacagaagaa cagcaggtcc tgcacgagcc
     1021 ggtcggagac ctccgactgg ccgcgctcgg ccaggcgcac ctgggccagc aggcgcgagg
     1081 tcaggcgctt ggcgtagacg tcggcgtcga gccggccctg cgcctgcgcc tcgaagaagc
     1141 cggcggcgat cgcccacagc ccggcgagct tggccgtgcc gcggtcggcc gagccggcgg
     1201 ccaggccggc gaacagccgg ctcatctgcg tggcggccgg accgaccggg ccgcgcagca
     1261 gcgcgagcat gcactgctcc atgcggccac gggtatcggc gtcggcgcgc agcggctcgg
     1321 cgccggagag cgcggactgc ccgagcgcct gctcgttggc cgccagcgcc gcgccctgcc
     1381 aggccccttc ggcctgccag aggtcggccg ggtggatgcg gtcggctccg gcgagcacct
     1441 gcacctcgca gtactgcggg aacagcgcga gcggcgagac cggcttgccg gcgagctggc
     1501 ggcgcaggta gtcgagcagc gcgaacgagg cgcgctcgat cgtcgtcgcc gccgcggcgt
     1561 ccaggaggcc gaagcgctgg ccgaagcgca gcgcggcctg ctcgctggcg cgcagcaggc
     1621 gcgcgccctc gggct
//
LOCUS       DASVLN010000041         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_378286, whole genome shotgun sequence.
ACCESSION   DASVLN010000041 DASVLN010000000
VERSION     DASVLN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378286"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1098
                     /locus_tag="VF453_00995"
     CDS             343..1098
                     /locus_tag="VF453_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7686243.1"
                     /translation="MRKSSDLEGIPRPVVALSDEYPAGFVDPPHRHERAQLLYAIAGV
                     MAVATADGSFVIPPQRAVWIPAGVEHEVSCRGPVSVRTLYIRPTEGLPTRCRVIEVSE
                     LMRALILEVMRFPAEYDERGREGLVVELLLKTIATLPVAPLHAPMPADPRVARICREL
                     LADPARDDSLDDWAAATHMSRRTLTRLFRQETGMSLAGWRQHVRLLEAVARLACGEAV
                     SKIAFEVGYDSPSAFTAVFQRVFGTAPSRYLVS"
     gene            1148..1642
                     /gene="nudB"
                     /locus_tag="VF453_01000"
     CDS             1148..1642
                     /gene="nudB"
                     /locus_tag="VF453_01000"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEX7686244.1"
                     /translation="MSADPTPAVRPPKIPESVLVVIHTPALEVLLIERADAPGYWQSV
                     TGSKDAADEPIVETCVREVAEETGIAIGSAQVPRSALLDCGLENVYEIYPRWRHRYAP
                     GVTHNTEHVFALEVPAGTPVVLAPREHTAFAWLPWREAADRCFSPSNAEAILHLPRWR
                     AGRR"
     gene            1816..2598
                     /locus_tag="VF453_01005"
     CDS             1816..2598
                     /locus_tag="VF453_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7686245.1"
                     /translation="MPPQTVTAGTRLRVATYNIHKGVRGVGAAKRLEIHNLALGVEAL
                     DADLVFLQEVRLHHTRDAARFDQTIMGWPDQGQAEFLAPEGYHSAYRTNAYTKHGEHG
                     NALLSRWPLGDVGHHDVSDHRFEQRGLLYVPVRWQDTTVHAIVAHFGLVHSSRMRQAQ
                     RLAEYIAAELPPDEPVLVAGDFNDWGERLDVPMSAAGLTRALASDPRAPKRPTFPSRL
                     PVFSLDRIYMRGFRCEGTMVPRGAGWARMSDHLPYVAELELN"
     gene            2789..>3370
                     /locus_tag="VF453_01010"
     CDS             2789..>3370
                     /locus_tag="VF453_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX7686246.1"
                     /translation="MRPKFAGGHAITLLRGGDALFPAMGAAIAQAKSSVWVATYIFHD
                     DAVAQTLALVLADAARRGVQVRVVVDGFGSKATLARLREWMVSAGVQLAVFRPIDRWW
                     SYFVPGQARRLHQKLCVVDGEVGFVGGVNLIADRNDLHHGWSDAPRLDFAAQLRGQAV
                     AEVEQAVQAIWTRAMLGRVWRDEVASLVRSAQPA"
BASE COUNT          429 a         1264 c         1215 g          462 t
ORIGIN      
        1 gcctcggccg gcgccagcgc ggacagcggc gggtgttgcg cataagccgc ggccaggcgg
       61 cgggcgaagg cgcgcatctc gggggccagc gcgaggccat cactgacctc gacgcgcgga
      121 atcagttcga tcgtggacac ggcgggcagg gcgcggcggg ggcgccacgg caggggaggg
      181 cgcttcgagc ataggcatgc tcgaccggcc gaagcttgcg cggctgggcc gatcgctgtc
      241 gagaacgggc cgctcgcgct cgagttggcg cagaatgctc gacaagtctt cgccctgtcc
      301 cgtcgccacc caccgccgca ccacgcggca tcggagacaa ccatgcgcaa gagcagcgat
      361 ctcgaaggca tcccgcgccc cgtggtggcg ctgtccgacg agtacccggc ggggttcgtc
      421 gacccgccgc atcgccacga gcgcgcccag ctgctctacg ccatcgccgg cgtgatggcg
      481 gtggccaccg ccgacggcag cttcgtcatc ccgccgcagc gcgccgtctg gattcctgcc
      541 ggcgtcgagc acgaggtgtc gtgccgcggc ccggtctccg tgcgcacgct ctacatccgg
      601 cccaccgagg gcctgcccac gcggtgccgg gtgatcgagg tctccgagct gatgcgggcg
      661 ctgatcctcg aggtgatgcg cttccccgcg gaatacgacg agcgcggccg cgagggcctg
      721 gtcgtcgagc tgctgctcaa gacgatcgcc acgctgcccg tcgcgccgct gcacgcgccc
      781 atgcccgccg acccgcgggt cgcgcggatc tgccgcgagc tgctcgccga tccggcgcgc
      841 gacgactcgc tggacgactg ggccgccgcc acgcacatga gccgccgcac gctcacgcgc
      901 ctgttccgcc aggagaccgg catgagcctg gccggctggc gccagcacgt gcgcctgctc
      961 gaggccgtcg cccgcctggc ctgcggcgag gccgtcagca agatcgcctt cgaggtcggc
     1021 tacgacagcc cgagcgcgtt cacggccgtc ttccagcggg tgttcgggac ggcgccgagc
     1081 cggtacctcg tctcgtagcc cgtgcgaggg ccggcgggcc ggccgccggc gcgctagcat
     1141 cgcgcccatg tccgccgacc ccacgcccgc cgtccgtccg cccaagattc ccgagtcggt
     1201 cctcgtggtg atccacacgc ccgcgctgga ggtgcttctg atcgagcgcg cggacgcgcc
     1261 ggggtactgg cagagcgtca ccggctcgaa ggatgccgcc gacgagccga tcgtcgagac
     1321 ctgcgtgcgc gaggtcgcgg aggagacggg catcgccatc ggttcggcgc aggtgccgcg
     1381 ctcggcgctg ctcgattgcg ggctcgagaa cgtctacgag atctacccgc gctggcgcca
     1441 ccgctacgcg cccggcgtca cgcacaacac cgagcacgtg ttcgcgctcg aggtgcctgc
     1501 gggcacgccc gtggtgctcg cgccgcgcga gcacacggcc tttgcctggc tgccctggcg
     1561 cgaggcggcc gaccgctgct tctcgccctc gaacgccgag gcgatcctgc acctgccgcg
     1621 ctggcgcgcc gggcgccgct gagcgggccg cgtccgcttc gccggccctc ttgtcatcgg
     1681 cgcgggcgcc cgcaagtggc atgatccggc ggcatgacga ttgcccgatg gtcggccctg
     1741 ggcccgctgt cgccgggcgt ccttcgaaac gaacgagcca tcgccgtgct gaatacgtcg
     1801 tcggacttca ccgtcctgcc gccccagacc gtcaccgccg gcacgcgcct gcgcgtcgcg
     1861 acctacaaca tccacaaggg cgtgcgcggc gtcggcgcgg ccaagcggct ggagatccac
     1921 aacctcgcgc tcggcgtgga ggcgctcgac gccgacctcg tcttcctgca ggaggtgcgc
     1981 ctgcaccaca cgcgcgacgc ggcccgcttc gaccagacca tcatgggctg gccggaccag
     2041 gggcaggccg agttcctcgc gccggagggc taccactcgg cctaccgcac gaacgcgtac
     2101 acgaagcacg gcgagcacgg caacgccctg ctgtcgcgct ggccgctggg cgacgtcggc
     2161 caccacgacg tctccgacca ccgcttcgag cagcgcggcc tgctgtacgt gccggtgcgc
     2221 tggcaggaca cgacggtgca cgcgatcgtc gcccatttcg ggctcgtgca ctcgagccgc
     2281 atgcgccagg cgcagcgact ggccgagtac atcgccgccg agctgccgcc cgacgagccg
     2341 gtcctcgtgg ccggcgactt caacgactgg ggcgagcgcc tggacgtccc gatgtccgcg
     2401 gccggcctga cgcgcgcgct ggcctccgac ccgcgcgccc ccaagcggcc gaccttcccg
     2461 tcgcggctgc cggtgttctc gctggaccgc atctacatgc gcggcttccg ctgcgagggc
     2521 acgatggtgc cgcgcggcgc gggctgggcg cgcatgtccg accacctgcc gtacgtggcc
     2581 gagcttgagc taaattgacg cctgccgcgc ttcgagcgcg gtagtcgtct tccgcgccgc
     2641 gccggcctgc gccggcacgc gcggccttgg ccctgcatcc gatccaatga gggagatccc
     2701 gattcgcctg aagcgccgcc tgcgacccga tgccgcacgg ccgcggccct cgcggggcgg
     2761 cgtcgccgcg ggccagtgga gcgtgggcgt gcggccgaag ttcgccggcg gccacgcgat
     2821 cacgctgctg cgcggcggcg acgcgctgtt ccccgcgatg ggcgcggcca tcgcgcaggc
     2881 gaagtccagc gtctgggtcg cgacctacat cttccacgac gacgccgtcg cgcagacgct
     2941 ggcgctcgtg cttgccgacg ccgcgcggcg cggcgtgcag gtgcgcgtgg tggtcgacgg
     3001 cttcggctcg aaggcgacgc tcgcgcggct gcgcgaatgg atggtgtccg cgggcgtgca
     3061 gctggcggtg ttccggccga tcgaccgctg gtggagctac ttcgtgcccg gccaggcgcg
     3121 gcggctgcac cagaagctgt gcgtggtcga cggcgaggtc ggcttcgtcg gcggcgtcaa
     3181 cctgatcgcc gaccgcaacg acctgcacca cggctggagc gacgcgccgc gcctggactt
     3241 cgcggcgcag ctgcgcggcc aggcggtcgc cgaggtcgag caggccgtgc aggcgatctg
     3301 gacgcgcgcg atgctgggcc gcgtctggcg cgacgaggtc gcgagcctcg tgcgcagcgc
     3361 ccagcccgcg
//
LOCUS       DASVLN010000042         2533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_432298, whole genome shotgun sequence.
ACCESSION   DASVLN010000042 DASVLN010000000
VERSION     DASVLN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432298"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VF453_01015"
     CDS             <1..376
                     /locus_tag="VF453_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686247.1"
                     /translation="ALAAGALAALASPAGAQVQRRFPPTALRGRIVFTSPPDIALNGD
                     LAHLAPGVRIHGPDNMMMVTGAVGGDGRRYDVDYRVEATGLVSEVWLLTPEEAAVKPW
                     PRTPAEAAAWTFDYMGQSWTKP"
     gene            510..1856
                     /gene="miaB"
                     /locus_tag="VF453_01020"
     CDS             510..1856
                     /gene="miaB"
                     /locus_tag="VF453_01020"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX7686248.1"
                     /translation="MAKKVYIRTFGCQMNEYDSDKMADVLNAADGYEKTDDVEQADLV
                     LYNSCSVREKAAEKMFSDLGRIKHLKAKGVLIGVGGCVASQEGAAIVERAPYVDLVFG
                     PQTLHRLPKMLADRRALSRPQVDISFPEIEKFDHLPPARVEGASAFVSVMEGCSKYCT
                     YCVVPYTRGEEVSRPFEDVLTEVAGLADQGLKEVTLLGQNVNAYRGKMGDTDEYADFA
                     LLLETIAEFDSIERIRFTTSHPKEFGQRLIDAYARIPKLVNHLHLPVQHGSDRILMAM
                     KRGYTALEYKSTIRKLRAVRPDIRLSTDFIVGFPGETDEDFDKLMKLVEDVEFDASFS
                     FIYSPRPGTPAANLQDLTGYPVKLKRLKTLQDHLEANVLRFSEALVGTTQKILVEGPS
                     RKSADDLMGRTECNRIVNFPGGPNAGRLVGRIVDARITQAFPHSLRGEVAVEERVA"
     gene            1912..>2533
                     /locus_tag="VF453_01025"
     CDS             1912..>2533
                     /locus_tag="VF453_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7686249.1"
                     /translation="MITDPAFAAHLWLYALLVLGIIALPGMDMAFVMASALADGRSSG
                     LVATAGFVCGGAVHVAMGATGVGLLLQHSPLAFDALLLAGGLYVAWIGVGLLRHAGAL
                     VALGDAPSRPLGHTFGRAVVTCLLNPKAYVFTIAVLPQFIVPRAGPVAAQAAVLWALG
                     AAAQVLVYGAVALTAARLRTWMRGSAAAQARLGRSVGLLLVAAAAMT"
BASE COUNT          375 a          931 c          862 g          365 t
ORIGIN      
        1 ggcgctcgcc gcgggcgccc tcgccgcgct cgcgtcgcct gccggcgccc aggtgcagcg
       61 ccgcttcccg cccaccgcgc tgcgcgggcg catcgtcttc acgagcccgc ccgacatcgc
      121 gctcaacggc gacctggcgc acctcgcgcc gggcgtgcgc atccacgggc ccgacaacat
      181 gatgatggtc accggcgcgg tcggcggcga cggcaggcgc tacgacgtcg actaccgcgt
      241 cgaggccacg ggactggtgt ccgaggtctg gctgctcacg ccggaggaag ccgccgtcaa
      301 gccctggccg cgcacgccgg ccgaggccgc cgcctggacc ttcgactaca tgggccagtc
      361 ctggacgaaa ccctgacgtc cgggccccgg cgcgcgtgac gcgcgcgtga gcccatcctt
      421 cgattcagac cacccgcccc gtccccgcga cggagcccat cgcgccgccg cccatctttc
      481 cgggccgcgc gccgctccgg agatccgcca tggccaagaa ggtctacatc cgcacgttcg
      541 gctgccagat gaacgagtac gactcggaca agatggccga cgtcctgaac gccgccgacg
      601 gctacgagaa gaccgacgac gtcgagcagg ccgacctcgt cctgtacaac agctgctcgg
      661 tacgcgagaa ggccgccgag aagatgttct ccgacctcgg ccgcatcaag cacctcaagg
      721 ccaagggcgt gctgatcggc gtcggcggct gcgtcgcgag ccaggagggc gcggccatcg
      781 tcgagcgcgc gccctacgtc gacctcgtct tcggcccgca gacgctgcac cgcctgccca
      841 agatgctcgc cgaccggcgc gcgctgtcgc ggccgcaggt cgacatcagc ttccccgaga
      901 tcgagaagtt cgaccacctg ccacccgcgc gcgtcgaggg cgcgagcgcc ttcgtctcgg
      961 tgatggaggg ctgctcgaag tactgcacct actgcgtcgt gccctacacg cgcggcgagg
     1021 aagtctcgcg gccgttcgag gacgtgctga cggaggtcgc cggcctcgcc gaccagggcc
     1081 tgaaggaagt cacgctgctc gggcagaacg tcaacgccta ccgcgggaag atgggcgaca
     1141 cggacgaata cgccgacttc gcgctgctgc tggaaaccat cgcggaattc gacagcatcg
     1201 agcgcatccg cttcacgacc agccatccca aggaattcgg ccagcgcctg atcgatgcct
     1261 acgcccgcat ccccaagctg gtgaaccacc tgcacctgcc cgtgcagcac ggctccgacc
     1321 gcatcctcat ggcgatgaag cgcggctaca cggcgctcga gtacaagagc acgatccgca
     1381 agctgcgcgc cgtgcgaccc gacatccgac tgtcgaccga cttcatcgtc ggcttccccg
     1441 gcgagaccga cgaggacttc gacaagctca tgaagctggt cgaggacgtc gagttcgacg
     1501 cgtccttcag cttcatctac agcccgcgcc cgggcacgcc cgcggccaac ctgcaggatc
     1561 tcacgggcta cccggtcaag ctgaagcgcc tgaagacgct gcaggaccac cttgaggcga
     1621 acgtcctgcg cttcagcgag gcgctggtcg gcacgacgca gaagatcctc gtcgagggcc
     1681 ccagccgcaa gagcgccgac gacctgatgg gccgcaccga gtgcaaccgc atcgtcaact
     1741 tccccggcgg cccgaatgcc gggcgcctcg tcggccgcat cgtcgacgcg cggatcacgc
     1801 aggcgttccc gcattcgctg cgcggcgaag tggcggtgga agagcgcgtg gcctgagcac
     1861 ggcgctaatc ctgacaccgg ttggcatgat tgggcgcgca cactgcgccg catgatcacc
     1921 gaccccgcct tcgccgccca cctctggctc tacgccctgc tcgtgctggg catcatcgcg
     1981 ctgcccggca tggacatggc gttcgtgatg gccagcgcgc tcgccgacgg gcggtcgtcg
     2041 gggctggtgg cgaccgcggg cttcgtctgc ggcggcgcgg tgcacgtcgc gatgggcgcg
     2101 acgggcgtcg ggctgctgct gcagcattcg cccctggcgt tcgacgcgct gctgctcgcc
     2161 ggcgggctgt acgtcgcgtg gatcggtgtc gggttgctgc gccatgcggg cgcgctggtg
     2221 gcgctcggcg acgcgccctc gcggccgctc gggcacacct tcggtcgcgc cgtcgtcacg
     2281 tgcctgctca accccaaggc ctacgtgttc acgatcgccg tgttgccgca gttcatcgtg
     2341 ccgcgggccg ggccggtggc tgcgcaggcc gccgtcctgt gggccctggg cgccgcggcg
     2401 caggtgctgg tctacggcgc ggtcgcgctg acggcggcgc gcctgcggac atggatgcgc
     2461 gggagcgcag cggcgcaggc gcggctcggg aggtcggtcg gcctgctgct ggtcgcggcg
     2521 gccgcgatga cgc
//
LOCUS       DASVLN010000043         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_163820, whole genome shotgun sequence.
ACCESSION   DASVLN010000043 DASVLN010000000
VERSION     DASVLN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163820"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF453_01030"
     CDS             <1..442
                     /locus_tag="VF453_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686250.1"
                     /translation="LAAARAIGGRVRVTEDVAAGVDGADAVYGDIWVSMGQEAQAGAL
                     VAALAPYRITVATMRATHNPRAIFLHCLPALHDDATQFGREHPDARDVDDEVFEGPAS
                     RVFDQAENRMHTIKALMLRTLAGDVDLGPLPALDPAPALADTEA"
     gene            444..875
                     /locus_tag="VF453_01035"
     CDS             444..875
                     /locus_tag="VF453_01035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEX7686251.1"
                     /translation="MRIRPFLDADEPAVIALWQACGLTRPWNDPARDIARKRAVQRDW
                     FLVGTLDERVVASAMAGYDGHRGWVFYVAVAPAARGRGCGRALMREVERLLREAGCPK
                     LNLLVRDDNAQALGFYRALGYVQDRVVSLGRRLIEDAPASS"
     gene            908..>2528
                     /locus_tag="VF453_01040"
     CDS             908..>2528
                     /locus_tag="VF453_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686252.1"
                     /translation="MAYDYSSENKRLELPNPYRVENAFLALCAVFTFAGGILGLTWAR
                     EAIAAHALRLGVAPLLVGAALVAASLGFAIVAARRLRFFFGRGRPRSLAPEVAVGATG
                     NSPRADQLKAMLRQGGLEYPEPHGALNGLLYHAVPRLITAPLVVQGLAQRQFFNALAF
                     VVTLASFACAWGLLGTAETRPLISIAYFLFGALVLLRPLVTGGEARLGTRELVALVVV
                     AVVGPVVAGLLGPALPSLRFAMAGQAFALLACALLAVVLILAALLAQVDEAPQTERSC
                     EQRTMSMNGPPGALVTELDRRLQDAWTEKIPNRRYTRLDPATESAAGSGRFAGELMEE
                     SQPMPVAGSAPSGLGVALEARRHRWLVVVDLYATLLVLAGTTCALVYVRGFDPARVLA
                     EGWGNPATIGYSGVCLAIAAFCFQSTNAIWGRFDFESVLVWVEMLGTWQASRISTGNQ
                     FASRLQTVNDVARVESMTLRVWRARVESVVFGKDGARQVTAMYATAQETARLAAELEA
                     FAARQSVFMAPRAQEDLHRIDALQATEAALGG"
BASE COUNT          288 a          933 c          945 g          362 t
ORIGIN      
        1 gctggcggcc gcgcgcgcga tcggcggccg cgtgcgcgtg accgaggacg tcgccgccgg
       61 cgtcgacggc gccgacgcgg tctacggcga catctgggtc tcgatgggcc aggaggcgca
      121 ggccggcgcg ctggtcgccg cgctcgcgcc ctaccgcatc accgtcgcga cgatgcgcgc
      181 gacgcacaac ccgcgcgcga tcttcctgca ctgcctgccg gcgctgcacg acgacgcgac
      241 gcagttcggc cgcgagcatc ccgacgcgcg cgacgtcgac gacgaggtct tcgagggccc
      301 ggccagccgc gtcttcgacc aggcggagaa ccgcatgcac acgatcaagg cgctgatgct
      361 gcgcacgctg gccggcgacg tcgacctcgg gccgctgccg gcgctcgacc ctgcgcccgc
      421 gctcgcggac acggaggcct gagatgcgca tccgcccctt cctcgacgcc gacgagccgg
      481 ccgtcatcgc gctgtggcag gcctgcgggc tcacccggcc ctggaacgac ccggcgcgcg
      541 acatcgcgcg caagcgcgcc gtgcagcgcg attggttcct cgtcgggacg ctggacgagc
      601 gcgtcgtcgc cagcgccatg gccggctatg acggccaccg cggctgggtc ttctacgtcg
      661 ccgtcgcgcc cgccgctcgc ggccgcggct gcgggcgcgc cttgatgcgc gaggtcgagc
      721 gcctgctgcg cgaggccggc tgcccgaagc tcaacctgct cgtgcgcgac gacaacgcgc
      781 aggcgctcgg cttctaccgc gcgctcggct acgtgcagga cagggtcgtg tcgctcggca
      841 ggcgcctgat cgaggacgcg cccgcgtcct cctagaatcg gcgccacctg cggcttcgga
      901 gcgacctttg gcctacgact acagctcgga gaacaagcgc ctcgagctcc cgaaccccta
      961 ccgcgtcgag aacgcgttcc tcgcgctgtg cgccgtgttc acgttcgcgg gcgggatcct
     1021 cggcctcacg tgggcgcgcg aggcgatcgc ggcgcatgcg ctgcggctgg gcgtcgcgcc
     1081 gctgctggtg ggcgcggcgc tggtcgcggc gagcctgggc ttcgccatcg tcgccgcgcg
     1141 gcggctgcgc ttcttcttcg gccgcggcag gccgcgctcg ctcgcgccgg aggtggccgt
     1201 cggcgcgacc ggcaactcgc cgcgcgccga ccagctgaag gcgatgctgc gccagggcgg
     1261 cctcgagtac ccggagcccc acggcgccct caacgggctg ctgtaccacg cggtgccgcg
     1321 cctgatcacc gcgccgctgg tcgtgcaggg cctcgcgcag cggcagttct tcaacgcgct
     1381 cgccttcgtc gtgacgctcg cgagcttcgc ctgcgcgtgg ggcctgctcg gcacggccga
     1441 gacgcggccg ctgatctcga tcgcctactt cctgttcggc gcgctggtgc tgctgcggcc
     1501 gctggtgacc ggcggcgagg cgcggctggg cacgcgcgag ctcgtcgcgc tcgtggtggt
     1561 ggccgtcgtc gggccggtgg tcgcggggct gctcgggccc gccctgccct cgctgcgctt
     1621 cgcgatggcg ggccaggcgt tcgcgctgct cgcgtgcgcg ctgctggccg tcgtgctgat
     1681 cctcgcggcg ctgctggcgc aggtcgacga ggcgccgcag acggagcgca gctgcgagca
     1741 gcgcacgatg tcgatgaacg gcccgccggg cgcgctggtc accgagctcg accgtcgcct
     1801 gcaggatgcc tggacggaga agatcccgaa ccgccgctac acgcgcctcg acccggcgac
     1861 cgagagcgcc gccggctccg gccgcttcgc cggcgagctg atggaggaat cgcagccgat
     1921 gccggtggcc ggcagcgcgc cgagcggcct cggggtcgcg ctcgaggcgc ggcgccatcg
     1981 ctggctcgtc gtcgtcgacc tctacgccac gctgctggtg ctggccggca cgacctgcgc
     2041 gctggtctac gtgcgcggct tcgacccggc gcgcgtgctc gccgagggct ggggcaaccc
     2101 ggcgaccatc ggctattcgg gcgtgtgcct ggcgatcgcc gcgttctgct tccagtcgac
     2161 gaacgcgatc tgggggcgct tcgacttcga gtcggtgctc gtgtgggtcg agatgctggg
     2221 cacctggcag gcctcgcgca tcagcacggg caaccagttc gcgtccaggc tgcagaccgt
     2281 caacgacgtc gcgcgcgtcg agtcgatgac gctgcgcgtc tggcgcgcgc gggtcgagtc
     2341 ggtggtgttc ggcaaggacg gcgcgcgcca ggtgacggcg atgtacgcga cggcgcagga
     2401 gaccgcgcgc ctcgccgccg agctcgaggc cttcgcggcg cgccagagcg tcttcatggc
     2461 gccgcgtgcg caggaggacc tgcaccgcat cgacgcgctg caggccaccg aggcggcgct
     2521 gggaggat
//
LOCUS       DASVLN010000044         2713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_378359, whole genome shotgun sequence.
ACCESSION   DASVLN010000044 DASVLN010000000
VERSION     DASVLN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378359"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VF453_01045"
     CDS             <1..354
                     /locus_tag="VF453_01045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX7686253.1"
                     /translation="RGLAALDAEAQAQAGVRFHAMAADAQDSLLRRVQHGQAYSPAWA
                     GMPPELFFRKRVLHDIVGAYYGHPTAWSEIGFGGPASPRGYVRLDFDRRDPWEASEAR
                     PGREDEARRENERVR"
     gene            401..1987
                     /locus_tag="VF453_01050"
     CDS             401..1987
                     /locus_tag="VF453_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009980099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX7686254.1"
                     /translation="MQRGAWTPMREYREDEDVDFLIVGTGAGGGTLACRLAEHGFSVV
                     ALDAGPYWRPLEDFASDETHQSKLYWTDERIVDGDDPLQLGANNSGKSVGGSTVHFAM
                     VSLRFRPEWFKSRTLLGYGADWPLDWREMWRYYDEVEQALCISGPVSYPWGPPRPRYP
                     YRAHQLNAAAQVLARGCEAMGIAWAPTPIATLSAPRGLAHPCVYRGMCTIGCSTNAKQ
                     SVLVTWLPRALAAGAEIRDLAMAGRIEHDAGGRATGVHYHRDGRWRFQRARNVVVAGY
                     AIETPRLLLNSASARHPDGLANSSGLVGRNLTVQANQAVWGVMDEEVRGYKAPPSLAI
                     TEHWNYTDRGKDFFGGYCYMSQGPLAVDWADKVAQRGLWGQPLIDEMTRYNHMTGLKI
                     VGEYLPQERNRVTLADERDALGLPVARVTYSCCDNDRRLVTHSLRFMREALAAAGGRE
                     IWDETDDTCHLAGTARMGDDPARSVVDADCRSWDVRNLWVCDGSVFPTAGGANPSLTI
                     QAIACRTADRIKAMAARGEL"
     gene            complement(1991..>2713)
                     /locus_tag="VF453_01055"
     CDS             complement(1991..>2713)
                     /locus_tag="VF453_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7686255.1"
                     /translation="MATIFAPALDIAHADFRRATEVTYLGGVWGVLAALRRMKPRGRG
                     TIVVVGSALAYRSIPLQAPYCGAKSALRGFVDALRCELAHERSGVHLTMVQLSAFNTP
                     QFDWGRTTLARQPRPMGKVFQPELAARGVYFAATHRRREVWVGWPAVQAILGQRVIPG
                     WLDRWLGRTAFAGQQTDEPLPPGRRDNLWEPVPGDHGAHGRFDDEAHATSLQFLLSKH
                     RGALATAGALLAAALLLGRRRR"
BASE COUNT          375 a         1004 c          995 g          339 t
ORIGIN      
        1 cgcgggctgg ccgcgctcga cgccgaggcc caggcccagg ccggcgtgcg cttccacgcg
       61 atggccgcgg acgcgcagga ctcgctgctg cgccgcgtcc agcatggcca ggcgtacagc
      121 cctgcctggg ccggcatgcc gcccgagctg ttcttcagga agcgcgtgct gcacgacatc
      181 gtcggcgcct actacggcca tccgaccgcg tggagcgaga tcgggttcgg cggcccggcg
      241 tccccccgcg gctacgtgcg cctggacttc gatcgccgcg atccgtggga ggcctcggag
      301 gcgcggccgg gccgcgagga cgaggcgcgg cgggagaacg aacgtgtccg atgatccgcg
      361 gcgcacgccg cgcgcgacag ccagccgcgc ccccgacgtc atgcagcgcg gggcctggac
      421 gccgatgcgc gagtaccgcg aggacgaaga cgtcgacttc ctgatcgtgg gcaccggtgc
      481 cggcggcggc accctcgcct gccggctcgc cgagcacggc ttctcggtcg tcgcgctcga
      541 tgccgggccg tactggcgac cgctggagga cttcgcctcc gacgagacgc accagtccaa
      601 gctgtactgg accgacgagc gcatcgtcga cggcgacgat ccgctgcagc tgggcgcgaa
      661 caactccggc aagagcgtcg gcggctcgac ggtgcatttc gcgatggtgt cgctgcgctt
      721 ccggccggag tggttcaagt cgcgtaccct cctggggtat ggcgccgact ggccgctgga
      781 ctggcgcgag atgtggcgct actacgacga ggtcgagcag gccctgtgca tctcgggccc
      841 ggtgagctac ccgtgggggc cgccgcggcc gcgctatccg taccgcgcgc accagctcaa
      901 cgccgcggcg caggtgctcg cgcgcggctg cgaggcgatg ggcatcgcgt gggcgccgac
      961 gcccatcgcc acgctgtcgg cgccgcgcgg gctcgcgcac ccctgcgtct accgcggcat
     1021 gtgcacgatc ggctgctcga cgaacgcgaa gcagagcgtc ctcgtgacct ggctgccgcg
     1081 cgcgctcgcc gccggcgccg agatccgcga cctcgcgatg gcaggccgca tcgagcacga
     1141 cgccggcggc cgcgccaccg gcgtgcacta ccaccgcgac ggccgttggc gcttccagcg
     1201 ggcgcgcaac gttgtcgtcg cgggctacgc gatcgagacg ccgcggctgc tgctgaactc
     1261 ggcgagcgcg cgccatcccg acgggctcgc gaactcctcg ggcctcgtcg gccgcaacct
     1321 gacggtgcag gccaaccagg ccgtctgggg cgtgatggac gaggaggtgc gcggctacaa
     1381 ggcgccgccc tcgctcgcga tcaccgagca ctggaactac accgaccgcg gcaaggactt
     1441 cttcggcggc tactgctaca tgagccaggg gccgctcgcc gtggactggg ccgacaaggt
     1501 ggcccagcgc gggctgtggg gccagccgct gatcgacgag atgacgcgct acaaccacat
     1561 gaccggcctg aagatcgtcg gcgagtacct gccgcaggag cgcaaccgcg tcacgctggc
     1621 cgacgagcgc gacgcgctgg ggctgccggt cgcgcgcgtc acctactcgt gctgcgacaa
     1681 cgaccgccgc ctggtgacgc actcgctgcg cttcatgcgc gaggccctcg cggccgcagg
     1741 cgggcgcgag atctgggacg agaccgacga cacctgccac ctcgccggca cggcgcgcat
     1801 gggcgacgat ccggcccgca gcgtggtcga cgcggattgc cgcagctggg acgtgcgcaa
     1861 cctgtgggtc tgcgacggct cggtcttccc gacggccggc ggcgcgaacc cgtcgctgac
     1921 gatccaggcc atcgcgtgcc gcaccgccga ccgcatcaag gcgatggcgg cgcgcggcga
     1981 gctctgaggt tcatcgccgg cgccgcccca gcagcagcgc cgcggccagc aacgcgcccg
     2041 ccgtcgcgag cgcgccgcgg tgcttcgaga gcaggaactg caggctcgtc gcatgcgcct
     2101 cgtcgtcgaa gcgtccgtgc gcgccgtggt cgccgggcac cggctcccag aggttgtcgc
     2161 gccgaccggg cggcagcggc tcgtcggtct gctggcccgc gaacgcggtg cggccgagcc
     2221 agcgatcgag ccagccgggg atcacgcgct gcccgaggat cgcctgcacc gcgggccagc
     2281 cgacccacac ctcgcgccgc cggtgcgtgg ccgcgaagta cacgccgcgc gcggcgagct
     2341 cgggctggaa gaccttgccc atcggccgcg gctggcgggc cagcgtggtg cggccccagt
     2401 cgaattgcgg cgtgttgaag gccgacagct gaaccatggt gaggtgcacg ccgctgcgct
     2461 cgtgcgcgag ctcgcagcgc agcgcgtcga cgaagccgcg cagcgccgac ttcgcgccgc
     2521 agtacggcgc ctgcagcggg atcgagcggt acgcgagcgc cgagcccacc accacgatcg
     2581 tcccgcgccc gcgcggcttc atgcgccgca gcgccgcgag cacgccccag acgccgccca
     2641 ggtaggtcac ctcggtggcc cggcggaagt ccgcgtgggc gatgtcgagc gccggcgcga
     2701 agatcgtcgc cat
//
LOCUS       DASVLN010000045         2228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_271422, whole genome shotgun sequence.
ACCESSION   DASVLN010000045 DASVLN010000000
VERSION     DASVLN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271422"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VF453_01060"
     CDS             <1..736
                     /locus_tag="VF453_01060"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX7686256.1"
                     /translation="PATPPLAPPPRITDLSHPMRPPPAPPMPPAGGGEGRLYSPGPFS
                     SIQVDGSGQVRVVQGDRDEVWVPGDEATQEAVDVHRSGSRLMLTLPGGWKFWRDSRTL
                     VEVRVRELHGVMLAGSADVLAPGPIATPELVVSVSGAGLARFDDLRADALRFDISGAG
                     DGQLAGQVGHLRLSVSGKGKVVANELRAQTADVSISGVANASLWVVDSLRVGISGAGH
                     VGYWGQPQVSQKISGFGGVEALGDKH"
     gene            complement(772..1449)
                     /locus_tag="VF453_01065"
     CDS             complement(772..1449)
                     /locus_tag="VF453_01065"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HEX7686257.1"
                     /translation="MKTRQALLGIVALALAGALGVASAQDHHGHDHDHDRGPGPGRPV
                     PGHGGPAFDARYHHDRYYPAHGYVTATLPLGAVSVGWHGGNYFFHGGVWWRPYGGRYV
                     VVEPPVGIFLPVLPTDYVTLWIGGAPYYYANGVYYSQVVGQGYEVVAPPPGADTAQPV
                     MAPPPPPPAPDPIIYPRNGQSAAQTEADRRECNSWATTQPSAQADAHVFQRAVEACMD
                     GRGYTVR"
     gene            complement(1591..>2228)
                     /locus_tag="VF453_01070"
     CDS             complement(1591..>2228)
                     /locus_tag="VF453_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686258.1"
                     /translation="GSLGDARAGDGTFTARLDPAGEGFGDYAGLIRTELTVQSGSTQG
                     YVAFDVVYTPGVPATWSGPAREALRDGSLDIDLPVDVAEAGRYVVTGRVDDANGQPFA
                     FVSFNDVLATGPQQVRLTVYGRLVRDQKPAFPLVLHDVDGFLLREDAYPDRVLMPARD
                     GVVYTTRRYTAASFSDAAYASDETARYLAEYGKDVAQAQQQVDQLQASTGP"
BASE COUNT          278 a          760 c          876 g          314 t
ORIGIN      
        1 gcccgcgacg ccgcccctgg cgccgccacc gcgcatcacc gatctctcgc acccgatgcg
       61 cccgccgccg gcgccgccca tgccgccggc gggcggcggc gagggccggc tctattcgcc
      121 cggtccgttc agcagcatcc aggtcgacgg ctcgggccag gtgcgcgtcg tgcagggcga
      181 ccgcgacgag gtctgggtgc ccggcgacga ggccacgcag gaggccgtcg acgtccatcg
      241 ctcgggctcc cggctcatgc tgaccttgcc cggcggctgg aagttctggc gcgacagcag
      301 gacgctcgtc gaggtgcgcg tgcgcgagct gcacggcgtg atgcttgcgg gctcggccga
      361 cgtgctcgcg ccggggccga tcgcgacgcc ggagctggtg gtgagcgtct cgggcgcggg
      421 cctcgcccgc ttcgacgacc tgcgcgccga cgcgctgcgc ttcgacatct cgggcgcggg
      481 cgacggccag cttgccgggc aggtcgggca tcttcgcctg agcgtgtcgg gcaagggcaa
      541 ggtggtcgcg aacgagctgc gggcacagac ggccgatgtc tccatcagcg gcgtcgccaa
      601 tgcgtcgctg tgggtggtcg acagcctgcg ggtcggcatc tccggcgccg ggcacgtcgg
      661 ctactggggc cagccgcagg tgagccagaa gatctccgga ttcggcggcg tcgaggcgct
      721 gggcgacaag cactgaggag ggcgcctgcg gcgcgccggc tcgctcgctg ctcagcgcac
      781 ggtgtacccg cgcccgtcca tgcaggcctc gacggcgcgc tggaagacgt gcgcgtcggc
      841 ctgggcgctc ggctgggtcg tcgcccagct gttgcattcg cggcgatcgg cctcggtctg
      901 cgcggcgctc tggccgttgc gcgggtagat gatcggatcg ggcgccggcg gcggaggcgg
      961 cggcgccatg acaggctgcg cggtgtcggc acccggcggc ggcgcgacga cctcgtagcc
     1021 ctggccgacg acctggctgt agtacacgcc gttggcgtag tagtagggcg caccgccgat
     1081 ccacagggtg acgtagtcgg tcggcagcac gggcaggaag atgcccaccg ggggctcgac
     1141 gacgacgtag cggccgccgt acggacgcca ccagacgccg ccgtggaaga agtagttgcc
     1201 gccatgccag ccgacgctga ccgcgcccag gggcagggtc gcggttacat agccatgagc
     1261 cgggtagtag cggtcgtggt ggtagcgggc gtcgaaggca gggccgccgt ggcccggcac
     1321 gggacggccg gggcccggcc cgcggtcatg gtcgtggtcg tggccgtgat gatcctgggc
     1381 cgacgcgacg ccgagcgcgc cggccagcgc cagcgcgacg atgcccagga gtgcctggcg
     1441 ggtcttcatg ctcaacctcc gcgatggggc ggcgcatgcc gccgggccat tgtcgacttg
     1501 cggtgtaatc gccgggttgc acgttgtcaa tgacgtgtga agcgccgcat ggcgcggagc
     1561 tgcccgccgc cggcgtgacc ggggactccg tcagggccct gtcgacgcct gcagctggtc
     1621 gacctgctgc tgcgcctggg cgacgtcctt gccgtattcg gccaggtagc gcgcggtctc
     1681 gtcgctcgcg taggcggcgt cgctgaagga cgcggcggtg tagcggcgcg tcgtgtagac
     1741 cacgccgtcg cgcgccggca tcagcacgcg gtcggggtag gcgtcctcgc gcaggaggaa
     1801 gccgtcgacg tcgtgcagca cgagcgggaa ggcgggcttc tggtcgcgca cgaggcggcc
     1861 gtacaccgtg agccggactt gctgcgggcc ggtcgcgagg acgtcgttga agctcacgaa
     1921 ggcgaagggc tggccgttgg cgtcgtcgac ccggcccgtg accacgtagc ggcccgcctc
     1981 ggccacgtcc acgggcaggt cgatgtcgag cgagccgtcg cgcagggctt cgcgcgcggg
     2041 gccgctccag gtcgccggaa cgcccggcgt gtagacgacg tcgaaggcga cgtagccctg
     2101 cgtgctgccg gactgcaccg tcagctcggt gcggatcagg ccggcgtagt caccgaagcc
     2161 ctcgccggcc gggtcgagcc gcgcggtgaa ggtgccgtcg cccgctcgtg cgtcgccgag
     2221 cgagccgt
//
LOCUS       DASVLN010000046         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_271436, whole genome shotgun sequence.
ACCESSION   DASVLN010000046 DASVLN010000000
VERSION     DASVLN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271436"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VF453_01075"
     CDS             complement(<1..680)
                     /locus_tag="VF453_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX7686259.1"
                     /translation="MTAAIAVAEDARPADRLADTIAGVSMAGLLLPEAVAYSGIAGTP
                     PQAGVFALFAGLVVYGLVGRSRFAIVSATSSSAAVLGAATLSLAGADALLRAALAAGL
                     VLATGAAFLAAAAFRLGSACSFISKPVLRGFSFGLAIVIVVKQLPAMTGVHPTADNAF
                     VYAAQLLAALPAWNPWALGLGMAALVALKALARFRKLPGALVVIALGIAASQGLQLAR
                     HGVGEVGA"
     gene            complement(769..1410)
                     /locus_tag="VF453_01080"
     CDS             complement(769..1410)
                     /locus_tag="VF453_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HEX7686260.1"
                     /translation="MTPTAHHHLVATGLRALRPTQITVGLAEVARKRAEWAALEKKAR
                     RKLLASHWFPSVLGPRGRHYIVDHHHLGLALLEEGVDEVGVLVLEDLSYLAPEVFWRV
                     MESRRWVHPFDRNGRRRGYDAIPESVRELVDDPHRSLAGFLRTAGGYAKDASPFAEFL
                     WADYLREHIARGQIRTQPEAALREALGLARSPLARYLPGWSGTIAPATALAAA"
     gene            1531..2325
                     /locus_tag="VF453_01085"
     CDS             1531..2325
                     /locus_tag="VF453_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7686261.1"
                     /translation="MQQQEQLIDIGVNFHSSQLRGVGDELVERASAAGVTGILATGTS
                     LAASRTELAFARRHARVWSTAGVHPHDAKTWDPTSAAAFEPLWADPKVVAIGECGLDY
                     NRMFSPKEDQRRAFEAQVEAAVRHGKPLFLHCRDAFVDFAAMLREAARAGAHGVVHCF
                     TDGVREAEAYLALGFDIGVTGWITDAARGTALRSAMRAIPLDRLHLETDAPYLRPRNA
                     GKTRPYNEPALLPFVASAVAELKGEPVSTVARHATRNSRRLFKLPD"
BASE COUNT          365 a          920 c          930 g          296 t
ORIGIN      
        1 atcgcgccga cctcgccgac gccatgccgc gcgagctgca gcccctgcga cgccgcgatc
       61 ccgagcgcga tgaccacgag cgcgcccggc agcttgcgaa agcgcgccag cgccttgagc
      121 gcgaccagcg ccgccatgcc caggccgagc gcccacgggt tccacgccgg cagcgcggcg
      181 agcagctgcg cggcgtagac gaacgcgttg tcggccgtcg ggtggacgcc ggtcatcgcg
      241 ggcagctgct tgaccacgat gacgatcgcc aggccgaacg agaatccgcg cagcaccggc
      301 ttcgagatga agctgcacgc cgacccgagc cggaacgccg ctgccgcgag gaaggccgcg
      361 ccggtggcca gcacgaggcc ggccgcgagc gccgcgcgca gcagggcgtc ggcgcccgcc
      421 agcgacagcg tcgccgcgcc gagcaccgcg gccgacgagg aggtcgccga cacgatcgcg
      481 aagcgcgagc gcccgaccag cccgtagacg accagccccg cgaacagcgc gaacacgccc
      541 gcctgcggcg gcgtgccggc gatgccggag taggccaccg cctcgggcag cagcaggccg
      601 gccatcgaga ccccggcgat cgtgtcggcg agccggtcgg cgggacgggc gtcttcggcg
      661 acggcgatcg ccgcggtcat gaggcgcgct ccgccgggcg ggggttcatg agacatcctc
      721 cgtcctgcgg actgcggtgc gagccccttc ggaacggccg ggtggggctc atgccgccgc
      781 cagcgccgtc gccggcgcga tggtgcccga ccatccgggc aggtagcgcg cgagcgggct
      841 gcgcgcgagg cccagcgcct cgcgcagcgc ggcctcgggc tgggtgcgga tctggccgcg
      901 ggcgatgtgc tcgcgcaggt aatcggccca caggaactcg gcgaagggcg atgcgtcctt
      961 ggcgtagccg ccggcggtgc gcaggaagcc ggcgaggctg cggtgcggat cgtcgaccag
     1021 ctcgcgcacg ctttccggga tcgcgtcgta gccgcggcgg cggccgttgc ggtcgaacgg
     1081 gtgcacccag cggcgcgact ccatcacgcg ccagaagacc tccggcgcga ggtaggagag
     1141 gtcctccagc accagcacgc ccacctcgtc gacgccttcc tccagcagcg cgaggccgag
     1201 gtggtggtgg tcgacgatgt agtgccggcc gcgcgggccg agcaccgacg ggaaccagtg
     1261 cgaggcgagc agcttgcggc gcgccttctt ctcgagcgcc gcccattccg cgcgcttgcg
     1321 ggcgacttcg gcgaggccga cggtgatctg cgtcggccgc agcgcgcgca gcccggtggc
     1381 gacgagatga tggtgggcgg tgggcgtcat ggcggcggcg acggggagcg gcgatggtgc
     1441 gcagcatacg gcgcacaggt gtggcgcggc ttgcacgttt cccccgcacg agggaaggcg
     1501 gcgcgccggg ggccccgcga taatcgaccg atgcaacaac aagaacaact gatcgacatc
     1561 ggcgtcaact tccattcctc ccagctgcgc ggcgtcggcg acgagctcgt cgagcgcgcg
     1621 agcgccgccg gcgtgacggg catcctcgcg accggcacct ccctcgcggc cagccgcacc
     1681 gagctcgcct tcgcccgccg ccatgcgcgc gtgtggtcca ccgccggcgt gcaccctcat
     1741 gacgcgaaga cgtgggaccc cacgagcgcc gccgccttcg agccgctctg ggccgatccc
     1801 aaggtcgtcg cgatcggcga atgcggcctc gactacaacc ggatgttctc gcccaaggag
     1861 gaccagcggc gcgcgttcga ggcgcaggtc gaggctgcgg tgcgccacgg caagccgctg
     1921 ttcctgcact gccgggacgc cttcgtcgac ttcgcggcga tgctgcgcga ggccgcccgc
     1981 gccggcgcgc acggcgtcgt gcactgcttc accgacgggg tgcgcgaggc cgaggcctac
     2041 ctggcgctcg gcttcgacat cggagtgacg ggctggatca ccgacgccgc gcgcggcacg
     2101 gcgctgcgca gcgcgatgcg cgcgatcccg ctcgatcgcc tgcacctgga gaccgacgcg
     2161 ccctacctgc ggcccaggaa cgccggcaag acgcggccgt acaacgagcc cgcgctgctg
     2221 cccttcgtcg cgagcgcggt ggccgagctc aagggcgagc ccgtgtccac ggtcgcccgg
     2281 cacgccacgc gcaacagccg gcgcctgttc aagctgcccg actgaaggcg gctccgcggc
     2341 cgctcgagca tgcggttcgt cggcgtttcg cgggcgttcg tcgacattcg ccacgcacta
     2401 ccggcttggg tatggcgatt gccaaaatcg ccgcgggcca cggtcgcgcc ggtcatgcgc
     2461 gacgcgcgcc ccgggagaac gaacttggga atcgatcgca cagacggacg g
//
LOCUS       DASVLN010000047         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_432486, whole genome shotgun sequence.
ACCESSION   DASVLN010000047 DASVLN010000000
VERSION     DASVLN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432486"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2134
                     /locus_tag="VF453_01090"
     CDS             <1..>2134
                     /locus_tag="VF453_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7686262.1"
                     /translation="QLAEARGALADRERTLAAERAVAQATLASTADAVLVTDESGRVL
                     AFNQEFLRIFGLAPDEVEGACTDALAPRIAARLRDGDATPQAVGLVGASGEGLGRVET
                     LDGRVLERSASEQGLQGHGGVRRVCSWRDVTARLEAEAALRDEAGILHFLNRTGAAIA
                     STLDMAALLQTVIDAATQVSGAAFGAFLYRDEPEAPGRVCGEGTPMLALAGVSLPPSE
                     QFDPMRLAALFGSAWRAGRTMRCDDISTEPRYRLTASWYGVHAGRAPLRSFLAVPVTL
                     RSGAIVGGLFFGHPDPGVFTGRTERIVAGVAAHAGIALDNARLVAGMQRAARERERLE
                     ASEREARAESVRADIIKDEFLSTLSHELRTPLTAILGWAKVLLLQRADAATRRRGLEA
                     IERNASAQVVMIEDLLDMSRIVSGKIRIEPRRCDLAAIADAALAGVRGSAQARRIVLE
                     HRVEPAARHVHGDPARLQQVIANLLSNALKFTPEGGRVELAVGRVDEQVEIAVTDTGI
                     GIEPEFVDHVFDQFRQADSSIRRRHGGLGLGLAIVRQLVALHGGAVGASSPGLGQGAR
                     FVVRLPAGDAPVEAARGDRAEAASPSGHFADVDLRGVRALVVDDDRDARELIAQLLLE
                     CGAEVVQAAGADEALAEFDRSPPDVLLSDIGMPGRDGYELIRAVRARGEARGGQIAAV
                     ALTAFARPEDRTRALLAGFQSHVAKPVDP"
BASE COUNT          241 a          795 c          834 g          264 t
ORIGIN      
        1 ccagctcgcc gaggcgcgcg gcgcgctcgc cgatcgcgaa cgcacgctgg cggcggagcg
       61 cgcggtggcg caggccacgc tggcgtccac cgcggacgcc gtcctcgtga ccgacgagtc
      121 gggacgcgtg ctcgccttca accaggagtt cctgcgcatc ttcgggctcg cgcccgacga
      181 ggtcgagggg gcctgcacgg acgccctcgc gccgcgcatc gccgcccgcc tgcgcgacgg
      241 cgatgcgacg ccgcaggccg tcggcctggt cggcgcgtcc ggcgaggggc tggggcgtgt
      301 cgagacgctg gacggccgcg tcctcgagcg ctcggccagc gagcagggcc tgcagggcca
      361 cggcggggtg cgccgcgtct gcagctggcg cgacgtgacg gcgcgcctcg aggcggaggc
      421 ggcgctgcgc gacgaggccg gcatcctgca cttcctcaac cgcaccggtg cggccatcgc
      481 gtcgaccctc gacatggccg cgctgctgca gacggtgatc gacgccgcca cccaggtcag
      541 cggcgccgcc ttcggcgcct tcctctaccg cgacgagccg gaggcgccgg gccgcgtctg
      601 cggcgagggc acgccgatgc tggcgctggc cggcgtgtcg ctgccgccgt ccgagcagtt
      661 cgacccgatg cgcctggccg cgctcttcgg gtcggcctgg cgcgcgggcc gcacgatgcg
      721 ctgcgacgac atcagcaccg agccgcgcta ccgcctgacc gcgagctggt acggcgtgca
      781 cgcggggcgc gcgccgctgc gcagcttcct cgccgtgccg gtgacgctgc gcagcggcgc
      841 gatcgtcggc gggctgttct tcggccatcc ggatcccggc gtgttcaccg ggcgcaccga
      901 gcgcatcgtg gccggcgtcg ccgcgcacgc gggcatcgcg ctcgacaatg cgcgcctggt
      961 cgccggcatg cagcgcgcgg cgcgcgagcg cgagcggctg gaggcctcgg agcgcgaggc
     1021 gcgcgcggag tcggtgcggg ccgacatcat caaggacgag ttcctctcca cgctctcgca
     1081 cgagctgcgc acgccgctga ccgcgatcct cggctgggcc aaggtgctgc tgctgcagcg
     1141 cgcggacgcg gcgacgcggc ggcgcgggct ggaggcgatc gagcgcaacg ccagcgccca
     1201 ggtcgtgatg atcgaggacc tgctggacat gagccgcatc gtctccggca agatccgcat
     1261 cgagccgcgg cgctgcgacc tcgcggcgat cgccgacgcc gcgctcgccg gcgtgcgcgg
     1321 cagcgcgcag gcgcggcgga tcgtgctgga gcaccgcgtc gagcccgccg cccgccacgt
     1381 gcacggcgac cccgcgcggc tgcagcaggt gatcgcgaac ctgctgtcga acgcgctgaa
     1441 gttcacgccg gagggcgggc gcgtcgagct ggcggtcggc cgcgtggacg agcaggtcga
     1501 gatcgcggtc acggacaccg gcatcggtat cgagccggag ttcgtcgacc acgtgttcga
     1561 ccagttccgg caggccgatt cgtcgatccg gcgccgccac ggcggcctcg gtctcgggct
     1621 ggcgatcgtg cggcagctcg tcgccctgca cggcggcgcg gtcggcgcga gcagccccgg
     1681 tctcgggcag ggcgcgcgct tcgtggtgcg cctgccggcc ggcgacgcgc cggtcgaggc
     1741 cgcgcgcggc gatcgtgccg aggccgcgtc cccgtcgggc cacttcgctg acgtggacct
     1801 gcgcggcgtg cgcgcgctgg tggtcgacga cgaccgcgac gcccgcgagc tgatcgcgca
     1861 gctgctgctc gagtgcggtg ccgaggtcgt gcaggccgcc ggcgccgacg aggccctggc
     1921 cgagttcgac cgcagcccgc ccgacgtgct gctgagcgac atcggcatgc cgggccggga
     1981 cggctacgag ctgatccgcg cggtgcgggc gcggggcgag gcgcgcggcg gccagatcgc
     2041 ggccgtggcc ctgaccgcct tcgcgcggcc ggaggatcgc acgcgcgcgc tgctcgccgg
     2101 cttccagtcg cacgttgcca agccggtcga cccg
//
LOCUS       DASVLN010000048         4930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_271604, whole genome shotgun sequence.
ACCESSION   DASVLN010000048 DASVLN010000000
VERSION     DASVLN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271604"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..769)
                     /locus_tag="VF453_01095"
     CDS             complement(26..769)
                     /locus_tag="VF453_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7686263.1"
                     /translation="MIRFEKVDKWYGDYHALVEVDESIAKGEVVVVCGPSGSGKSTLI
                     RTINRLEDIAGGRILMDGQDIHARGVDVNALRSRIGFVFQQFNLFPHLTVLQNCTLAP
                     VELRRLPPAQAREEAMALLDRVGLAHKANEHPSALSGGQQQRVAIARALAMRPPVMLF
                     DEPTSALDPEMVGEVLLVMRDLVRDGMTLVVVTHEMGFAREVAHRVLFMDAGRILERA
                     EPHEFFDHPRHPRAQQFLADIRTPFARPA"
     gene            complement(780..1523)
                     /locus_tag="VF453_01100"
     CDS             complement(780..1523)
                     /locus_tag="VF453_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7686264.1"
                     /translation="MLDVIRDYWLYFLVGQYPAGPLGGLALTVALASLALVGAMPVGV
                     ALGLARISPWRALRWPVTAIVYVVRGVPLLMVIFWAYFFLPSVTGVKTGQFSTMLAAL
                     VLFDGVYLAEIVRAGIRGVPRGQVEAARALGLGWGRTMRRVVLPQALRSMLPSLVNQF
                     VSTIKETSLGYIIGLSEVSFIASQINTQVFTHATAVYGVLALTYFVLCFGLSRIAFLL
                     ERRMNRRPGASRPRDELVAPANPLAAPNA"
     gene            complement(1531..2229)
                     /locus_tag="VF453_01105"
     CDS             complement(1531..2229)
                     /locus_tag="VF453_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7686265.1"
                     /translation="MQSFDLALLLHGQYHDWLMAGLRVSLGLAATTFVLALPLAVAVA
                     LLRLAPSRLARAAGAAFVEGIRNVPLLAHLLFWYFGAPQLLPDSWQQRLNDWGHMEFL
                     AAAIALGLYTAAYMAEDIRSGIRAIPAVQMEAGRALGFGFLATMRRVILPQALRVTVP
                     PLISQALNLWKDTSIATVIAVTEMMYQAARVESASFRSAEAFAFATAAYLAVSLAITG
                     LGAWYHRRYPVRTL"
     gene            complement(2246..3079)
                     /locus_tag="VF453_01110"
     CDS             complement(2246..3079)
                     /locus_tag="VF453_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7686266.1"
                     /translation="MRLALTLRSLLAAAIVATGLVSAAHADQLDDIKKKGELVVGVLG
                     TDEPNSFLDPKTREIVGYEVDLAKAIAAKIGVKPVFKQLAVAARIPELQQGRVDLLAA
                     SLTHNKERESQVDFSLTTFVTGQKAIVKNSSGIRDIAQLAGKKVLTVKGGTQEGNIRK
                     AVPTADVVTFETSQQGFLALQQGKGAAFVDDEAALVDDWAKLGDARKAYTILPTSISV
                     EPLALGLRKNEPRFKEVVDDTLRGLESSGEANRIFLKWYGPDTKLHFDHRGFKIDSDR
                     I"
     gene            3362..4642
                     /locus_tag="VF453_01115"
     CDS             3362..4642
                     /locus_tag="VF453_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7686267.1"
                     /translation="MTAFTEDQARAYMHKLLTAMAQAGGSDLFIACDFPPSMKIDGAM
                     KPLTSQKLTGEVTKALAAAMMNERQREEFARDLECNFAVTVAGVQRFRINVMLQQQSV
                     TMVCRAIASEIPNFEKLKLPEVLKDVVGAKRGLVLVVGATGSGKSTSLAAMIDHRNRT
                     QAGHIITVEDPVEYVHASRMSLVTHREVGVDTHGWHHALKNTLRQAPDVILIGEIRDA
                     ETMEHAIAFAETGHLCLGTLHANSASQTIERVINFFPEERRAQLLMDLSANLRAIVSQ
                     RLVRREDGKGRAAAIEILLNTPTIAEKIFTGAFNEIKGIMGRSRELGMCTFDWALFEL
                     YEARLIGFEEAIRNADSANELRLNIKLKSRRGEPGAFDGGGLTLDMGPSPEEIAARQE
                     EEERKAREARRKAEDEELARLRERKRLAEAAQGR"
     gene            complement(4657..>4930)
                     /locus_tag="VF453_01120"
     CDS             complement(4657..>4930)
                     /locus_tag="VF453_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686268.1"
                     /translation="PATVDEARRLLALVAPLARAPALEVAPDGRILLEWEAAEHGWLR
                     LAVDGTGRVAHEAVIGEDEFEQSEPFAHDLPDWAATLLARLMRAGH"
BASE COUNT          778 a         1707 c         1789 g          656 t
ORIGIN      
        1 ccggcaccgc ggccgccggg cgtcgtcagg ccggccgcgc gaacggcgtg cggatgtcgg
       61 cgaggaactg ctgggcgcgc gggtggcgcg ggtggtcgaa gaattcgtgc ggctcggcgc
      121 gctccaggat gcggccggcg tccatgaaca gcacgcggtg agcgacctcg cgcgcgaagc
      181 ccatctcgtg cgtgacgacc accagcgtca tgccgtcgcg caccaggtcg cgcatgacca
      241 gcagcacctc gcccaccatc tcggggtcga gcgcgctggt gggctcgtcg aacagcatca
      301 ccggcggccg catcgcgagc gcgcgcgcga tcgcgacgcg ctgctgctgg ccacccgaca
      361 gcgccgacgg gtgctcgttc gccttgtgcg cgaggccgac gcgatccagc agcgccatcg
      421 cctcctcgcg cgcctgtgcg ggcggcagcc tgcgcagctc gacgggcgcg agcgtgcagt
      481 tctgcagcac ggtcaggtga gggaagaggt tgaactgctg gaacacgaag ccgatgcgcg
      541 agcgcagcgc gttgacgtcg acgccgcggg cgtggatgtc ctggccgtcc atcaggatgc
      601 ggccgccggc gatgtcctcc agccggttga tcgtgcggat cagcgtcgac ttgcccgagc
      661 ccgagggccc gcacacgacc acgacctcgc ccttggcgat gctctcgtcg acctcgacaa
      721 gcgcgtggta gtcgccgtac cacttgtcga ccttctcgaa gcggatcatg gggccggtgt
      781 caggcattgg gcgcggcgag cgggttggcg ggggcgacga gctcgtcgcg gggacggctg
      841 gcacccgggc ggcggttcat gcggcgctcg agcaagaagg cgatgcgaga cagcccgaag
      901 cacagcacga agtaggtgag cgcgagcacg ccgtagaccg cggtggcgtg ggtgaagacc
      961 tgggtgttga tctgcgaggc gatgaacgag acctccgaca gcccgatgat gtagccgagc
     1021 gaggtctcct tgatcgtgga gacgaactgg ttgaccagcg aaggcagcat gctgcgcagc
     1081 gcctggggca gcacgacgcg tcgcatcgtc cgtccccagc cgaggccgag cgcgcgcgcc
     1141 gcctcgacct gcccgcgcgg cacgccgcgg atgccggcgc gcacgatctc ggcgaggtag
     1201 acgccgtcga acagcaccag cgccgcgagc atggtgctga actgcccggt cttcacgccc
     1261 gtgacgctgg gcaggaagaa gtaggcccag aagatgacca tcagcagcgg cacgccgcgc
     1321 acgacgtaca cgatcgccgt gaccggccag cgcagcgcgc gccaggggct gatgcgcgcc
     1381 agccccagcg cgacgcccac cggcatcgcg ccgacgagcg cgagcgaggc gagcgccacc
     1441 gtcagcgcga gtccgcccag cggcccggcc gggtactggc cgacgaggaa gtagagccag
     1501 tagtcgcgga tcacgtcgag catcgcggcc tcacagcgtg cggacgggat accgccggtg
     1561 gtaccacgcg ccgaggccgg tgatcgccag cgacacggcc aggtaggccg ccgtcgcgaa
     1621 ggcgaaggcc tcggcgctgc ggaagctggc ggactccacc cgcgcggcct ggtacatcat
     1681 ctcggtgacc gcgatgacgg tggcgatgct cgtgtccttc cacaggttca gcgcctgcga
     1741 gatcagcggc ggcaccgtca cccgcagcgc ctgcggcagg atcacgcgcc gcatcgtcgc
     1801 gaggaagccg aatcccagcg cgcgcccggc ctccatctgc acggcgggga tggcgcggat
     1861 gccgctgcgg atgtcctcgg ccatgtaggc ggccgtgtac agcccgaggg cgatcgccgc
     1921 ggcgaggaac tccatgtggc cccagtcgtt gagccgctgc tgccaagaat cgggaagcag
     1981 ctgcggcgcg ccgaagtacc agaacagcag gtgcgcgagc agcgggacgt tgcggatgcc
     2041 ctcgacgaag gcggcccccg ccgcgcgcgc gagccggctg ggcgcgagcc gcagcagcgc
     2101 caccgcgacc gcgagcggca gcgcgagcac gaaggtcgtc gcggccagtc ccagggagac
     2161 gcgcagcccc gccatcagcc agtcgtggta ctggccgtgc agcaggagcg cgaggtcgaa
     2221 cgactgcaag ggacgagtcg cgccgtcaga tgcggtcgga gtcgatcttg aagccgcggt
     2281 ggtcgaagtg cagcttggtg tccgggccgt accacttcag gaagatcctg ttcgcctcgc
     2341 ccgaggattc caggccgcgc agcgtgtcgt cgacgacctc cttgaagcgc ggctcgttct
     2401 tgcgcaggcc gagcgcgagc ggctcgacgc tgatgctggt cggcaggatg gtgtacgcct
     2461 tgcgggcatc gcccagcttg gcccagtcgt ccaccagcgc cgcctcgtcg tcgacgaagg
     2521 cggcgccctt gccctgctgc agcgcgagga agccctgctg cgaggtctcg aaggtcacga
     2581 cgtcggccgt cggcacggcc ttgcggatgt tgccttcctg ggtaccgccc ttgaccgtca
     2641 gcaccttctt gccggcgagc tgggcgatgt cgcggatgcc gctggagttc ttcacgatcg
     2701 ccttctggcc ggtgacgaag gtggtcagcg agaagtcgac ctgcgactcg cgctccttgt
     2761 tgtgcgtcag cgaggcggcc aggaggtcga cgcggccctg ctgcagctcg gggatgcggg
     2821 cggcgacggc gagctgcttg aacacgggct tgacgccgat cttcgccgcg atggccttgg
     2881 cgaggtcgac ctcgtagcca acgatctcgc gcgtcttcgg atcgaggaag ctgttgggct
     2941 cgtcggtgcc cagcacgccc acgacgagct cgcccttctt cttgatgtcg tcgagctggt
     3001 cggcgtgggc ggcggagacg aggcccgtcg cgacgatcgc ggcggcgagc agggagcgga
     3061 gcgtgagggc gaggcgcatg gcggcggttc cttgttgtcg agcggcgagc gtaggcgggc
     3121 tgcggcccgt cgtgaagcga cggttgcgca tatccatcct cccggcgatg ccgggcggtc
     3181 gccggaacgc cgggccggcg accggcgcag gatagcggaa ccggcgcgag cgtgccgatt
     3241 tcacgccgat gtccgaaatg cgcggccgca cgcttgagcg ggcggggggc ccgccgatga
     3301 accgggcgac gtgcgccggc ccgggtcggc gctccccgcc atcccccgac gccggagttc
     3361 catgaccgcc ttcaccgaag accaggcccg cgcctacatg cacaagctgc tcaccgccat
     3421 ggcccaggcc ggcggctcgg acctgttcat cgcctgcgac ttcccgccca gcatgaagat
     3481 cgacggcgcc atgaagccgc tgacctcgca gaagctcacg ggcgaggtga cgaaggcgct
     3541 cgcggccgcg atgatgaacg agcgccagcg cgaggagttc gcgcgcgacc tcgagtgcaa
     3601 cttcgccgtc acggtcgccg gcgtccagcg cttccgcatc aacgtgatgc tgcagcagca
     3661 gtcggtcacc atggtctgcc gtgcgatcgc ctccgagatc ccgaacttcg agaagctcaa
     3721 gctgcccgag gtgctcaagg acgtcgtcgg cgccaagcgc gggctggtgc tggtcgtcgg
     3781 cgcgaccggc tcgggcaagt cgacgtcgct cgccgcgatg atcgaccatc gcaaccgcac
     3841 ccaggccggc cacatcatca cggtggagga cccggtcgag tacgtgcacg cgtcgcgcat
     3901 gtcgctggtg acgcatcgcg aggtgggcgt cgacacccac ggctggcacc acgcgctgaa
     3961 gaacacgctg cgccaggcgc ccgacgtgat cctgatcggc gagatccgcg acgccgagac
     4021 catggagcac gcgatcgcct tcgccgagac cggccacctg tgcctgggca cgctgcacgc
     4081 caactccgcg agccagacga tcgagcgcgt catcaacttc ttccccgagg agcgccgcgc
     4141 ccagctgctg atggacctgt cggccaacct gcgggccatc gtctcccagc gcctggtgcg
     4201 gcgcgaggac ggcaagggcc gcgcggcggc gatcgagatc ctgctgaaca cgcccaccat
     4261 cgccgagaag atcttcacgg gcgcgttcaa cgagatcaag ggcatcatgg gccgctcgcg
     4321 cgagctgggc atgtgcacct tcgactgggc gctgttcgag ctctacgagg cgcgcctgat
     4381 cggcttcgag gaggcgatcc gcaacgccga ctcggccaac gagctgcgcc tgaacatcaa
     4441 gctcaagagc cgccgcggcg agcccggcgc cttcgacggc ggcggcctga cgctcgacat
     4501 gggcccgtcg cccgaggaga tcgcggccag gcaggaggag gaagagcgca aggcccgcga
     4561 ggcgcgccgc aaggccgagg acgaggaact cgcccgcctg cgcgagcgca agcgcctggc
     4621 agaggcagcg caggggcgct gaagcgccgc cgcccgtcag tgcccggcgc gcatcagccg
     4681 cgcgagcagg gtcgcggccc agtcgggcag gtcgtgcgcg aagggctccg actgctcgaa
     4741 ctcgtcctcg ccgatcacgg cctcgtgggc gacgcggccc gtcccgtcga ccgccaggcg
     4801 cagccagcca tgctccgcgg cttcccactc gagcaggatg cggccgtcgg gcgcgacctc
     4861 cagcgccggc gcgcgcgcga gcggcgccac cagcgccagc agccggcgcg cctcgtcgac
     4921 ggtcgcgggc
//
LOCUS       DASVLN010000049         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_110141, whole genome shotgun sequence.
ACCESSION   DASVLN010000049 DASVLN010000000
VERSION     DASVLN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110141"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1826
                     /locus_tag="VF453_01125"
     CDS             <1..>1826
                     /locus_tag="VF453_01125"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX7686269.1"
                     /translation="LVFAEGRFDPSDPAGRRLLAHELAHVAQDGAPMLRRDDDAPGTA
                     PAVDPDDPTTEPDADEDLAPDSLEGVQAEWDAMQDALQAAADQQGNDDYAAIDAEDRQ
                     GRPRRRDQLGLAFWLMTLPPSAYLRTIVVTDFWVERDADLVANLADVDAVDATFAKAL
                     DIAADEDRTIALLGPRADHYERDRPEAFPAFWREWLRRALEPDAREVDLVAKAAAAAD
                     EMGRQGQRLQDGIFAQGLPVAFNDAMTMVSFTLVPELAREPVRHPVERFAQAALAWGY
                     LQSLALFTRDWNRLAAGMVLAFGQGTLAFKLDELLDLKERQVSRLRDVDSLLHNGWMS
                     AVDRKVFPPVPDGIAVRLGSYRFEAGFSAYDGLWITPRGIFDAALRATDADIAHASEE
                     GRVAIGARWESALGYRDAAWDAVKSGLWKQAPETVVTGGAFALAHAVAPEVMVFVDLF
                     YAAKAAWDAGIEVPSEIEDALALVRSATTVVDLMRTQAQLSMTQVGGLTRQVLDAIAI
                     RGAAKGARAHFSGAATEGLADAEGEASEALSTRLARTRERAAVRRETEDIVDRRLDDR
                     QVAAEIEEAGQHAPHDSSEAGYDEQIDLPNKHQWRRRRGDGQ"
BASE COUNT          245 a          683 c          670 g          228 t
ORIGIN      
        1 ctcgtcttcg ccgagggacg cttcgatccc tccgaccccg ccggccgccg cctgctcgcg
       61 cacgagctcg cccacgtcgc gcaggacggc gcgccgatgc tgcgccgcga cgacgacgcg
      121 cccgggaccg cgcccgccgt cgacccggac gacccgacga ccgagcccga tgcggacgag
      181 gatctcgcgc ccgactcgct cgaaggcgtc caggccgaat gggacgcgat gcaggacgcc
      241 ctgcaggccg ccgccgacca gcagggcaac gacgactacg ccgccatcga cgccgaggac
      301 cgccaggggc gcccgcgccg ccgcgaccag ctgggccttg cgttctggct gatgaccctg
      361 cctccctcgg cctacctgcg cacgatcgtc gtcaccgact tctgggtcga gcgggacgcc
      421 gacctcgtcg cgaacctggc cgacgtggac gcggtcgacg ccacgttcgc caaggccctc
      481 gacatcgcgg ccgacgagga ccgcacgatc gcgctgctcg gcccgcgcgc cgaccactac
      541 gagcgcgacc gtccggaggc cttccccgcg ttctggcgcg agtggttgcg gcgcgcgctg
      601 gagcccgacg cgcgcgaggt cgacctcgtc gccaaggcgg cggccgccgc cgacgagatg
      661 ggccggcagg ggcagcgcct gcaggacggc atcttcgccc agggcctgcc ggtggcgttc
      721 aacgacgcga tgacgatggt ctcgttcacg ctcgtgcccg agctcgcgcg cgagcctgtg
      781 cgccatccgg tcgagcgctt cgcccaggcg gcgctggcgt ggggctacct gcagtcgctg
      841 gcgttgttca cgagggactg gaaccgcctc gccgccggca tggtgctcgc gttcggccag
      901 ggcacgctgg cgttcaagct cgacgagctc ctcgacctga aggagcgcca ggtctcgcgc
      961 ctgcgcgacg tcgactcgct gctgcacaac ggctggatgt cggcggtcga ccgcaaggtc
     1021 ttcccgcccg tgcccgacgg catcgcggtg cggttgggca gctaccgctt cgaggccggc
     1081 ttctcggcct acgacggcct gtggatcacg ccgcgcggga tcttcgacgc cgcgctgcgc
     1141 gccaccgacg ccgacatcgc gcacgcgagc gaggaaggcc gcgtcgcgat cggcgcgcgc
     1201 tgggagtcgg ccctcggcta ccgcgacgcg gcctgggacg cggtcaagag cggcctgtgg
     1261 aagcaggcgc cggagacggt cgtgaccggc ggggccttcg cgctcgcgca cgcggtggcg
     1321 ccggaggtga tggtgttcgt cgacctcttc tacgcggcca aggcggcgtg ggacgcgggc
     1381 atcgaggtgc cgtcggagat cgaggacgcg ctggcgctcg tgcgctcggc caccaccgtc
     1441 gtcgacctga tgcgcacgca ggcgcagctg tccatgacgc aggtcggcgg gctcacgcgc
     1501 caggtgctcg acgcgatcgc gatccgcggc gcggccaagg gcgcccgggc gcatttctcg
     1561 ggggcggcca ccgaggggct ggccgacgcc gagggcgagg cctcggaggc cttgtccacg
     1621 cgcctcgccc gcacgcgcga gcgcgccgcc gtccggcgcg agacggagga catcgtcgac
     1681 cgccgcctcg acgatcgcca ggtcgccgcc gagatcgagg aagccgggca gcatgcgccg
     1741 cacgattcgt ccgaggcggg ctacgacgag cagatcgacc tgcccaacaa gcaccagtgg
     1801 cggcgccgcc gcggcgacgg gcagtg
//
LOCUS       DASVLN010000050         6911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_217415, whole genome shotgun sequence.
ACCESSION   DASVLN010000050 DASVLN010000000
VERSION     DASVLN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217415"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VF453_01130"
     CDS             <1..740
                     /locus_tag="VF453_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686270.1"
                     /translation="QREQERVLALLEDRPWHPDAPPTASIVPPPLPQPVLAQADFGVV
                     HPTRDARHMADWVVARHDNGRLPFIVLDKRAARLYVFQPNGTLVQDTPVLLGQSHGDD
                     TYPGIGNVPISEVKPYQRTTAAGRFVTRPGLDEDHTDVVWLDYDAALAMHRVINKVKS
                     ERRPWRLAQPNPKLRRISWGCINIPIPFFNQYISPVFGKQRGVVYVIPETKTFAEVFE
                     KNGGAAAVGQTLAMDNAPAAPKAVVDR"
     gene            823..1692
                     /locus_tag="VF453_01135"
     CDS             823..1692
                     /locus_tag="VF453_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7686271.1"
                     /translation="MSHPRAFIWRVLVAFKANQGLLLAGAVAYYALLSIVPLLILSVI
                     ALAHVIPEHELLETIGRYLAWLVPSQSGAVVAELRGFLDHREVVGWFLAVTMLFFSSL
                     AFAVLENALSVVFVHRVSHRKRTLLARLLMPYVYVLCLGFGLMMVTLVAGGLEELGQR
                     SIDFLGHHWSLGFLSRLLLYLLGLSGEIVLLTSIYVVMPESPLDVRHALIGAVSAVAL
                     WEVTRHVLVWYFATLSQVGVVYGSMTTAIVVLLSLELGATLVLLGAQVIAEYERLERH
                     APPAREPELVQRR"
     gene            1935..>6911
                     /locus_tag="VF453_01140"
     CDS             1935..>6911
                     /locus_tag="VF453_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX7686272.1"
                     /translation="MTIMETRPALEPVSEMHQLLGALVALRKGDADVRLPVHWSGVAG
                     RVADAFNDVVEQNANMAEELARLRQMVGKEGKLKQRASLREAKGFWGESIDCINSLID
                     DLVHPTSEVARVIGAVAQGDLSKSMALEVDGRPLEGEFLRTAKTINKMVDQLGNFSAE
                     VTRVAREVGTEGKLGGQAKVKGVAGTWKDLTDSVNSMAGNLTDQVRNIADVTTAVAKG
                     DLSKKIDVDVKGEFLTLKNTINTMVDQLRSFASEVTRVAREVGTEGSLGGQARVEGVS
                     GTWKDLTDSVNSMAGNLTSQVRNIADVTKAVAAGDLSKKITVDVKGEILELKNTVNTM
                     VDQLRSFAAEVTRVAREVGTEGKLGGQADVQGVAGTWKDLTDSVNSMAFNLTSQVRNI
                     ADVTKAVAAGDLSKKITVDVKGEISELKTTINTMVDQLSSFAAEVTRVAREVGTEGRL
                     GGQAEVKGVSGTWKDLTDSVNSMAGNLTSQVRNIADVTKAVAAGDLSKKITVDVKGEI
                     LELKNTINTMVDQLRSFAAEVTRVAREVGTEGKLGGQADVQGVAGTWKDLTESVNFMA
                     GNLTSQVRNIAEVTTAVAAGDLSKKITVDVKGEILELKNTINTMVDQLRSFAAEVTRV
                     AREVGTEGKLGGQADVQGVAGTWKDLTESVNFMAGNLTSQVRNIAEVTTAVAAGDLSK
                     KITVDVKGEISELKNTINTMVDQLSSFAAEVTRVAREVGTEGKLGGQADVQGVAGTWK
                     DLTESVNSMASNLTSQVRNIADVTKAVAAGDLSKKISVDVQGEISELKSTINTMVDQL
                     RSFAAEVTRVAREVGTEGILGGQADVQGVAGTWKDLTESVNFMAGNLTSQVRNIAEVT
                     TAVAAGDLSKKITVDVKGEISELKNTINTMVDQLRSFAAEVTRVAREVGTEGKLGGQA
                     DVQGVAGTWKDLTDSVNLMASNLTSQVRNIADVTKAVAAGDLSKKITVDVKGEILELK
                     ATINTMVDQLSSFAAEVTRVAREVGTEGRLGGQAQVQGVSGTWKDLTDNVNFMAGNLT
                     SQVRGIAKVVTAVADGDLERKLTVEAKGEIAALADTINGMTDTLATFADQVTTVAREV
                     GVEGKLGGQAKVPGASGTWKALTENVNELAANLTTQVRAIAEVATAVTQGDLTRSITV
                     ETQGEVASLKDTINEMIRNLRDTTQKNTEQDWLKTNLAKFSRMLQGQRDLVAVGQLIL
                     SELAPVVGAQQAEFYVMGSSAEAPKLRLIASYASGGQATHGKEVHLGEGLVGQCAVDK
                     RKLLLTNVPSTAFRIASGLSESTAMDVLVLPVVFEGQMRGVLELASLERFNPVHEAFL
                     DQLTESIGIVINTIEANSRTEDLLKQSQSLAEELGSRQKELQQTNQELQDKARLLAHQ
                     NQEVERKNTEVEQARQALEEKAEQLALTSKYKSEFLANMSHELRTPLNSLLILSDQLC
                     KNAEGNLTPKQVEFSKTIHSSGNDLLMLINDILDLSKIESGTVAVDASELRVDDLHRS
                     VERGFRHFAESKHVDFVVELRQPLPKTMVTDVKRLQQIIKNLLSNAFKFTHEGQVRFT
                     IATATGGWSADNEELARAAQVLAFSVADTGIGISPDKQQIIFEAFQQADGSTSRKYGG
                     TGLGLAISRELSRLLGGEIRLQSEPGRGSEFTLYLPQAYSPARTARHVR"
BASE COUNT         1212 a         2272 c         2458 g          969 t
ORIGIN      
        1 cgcagcgcga gcaggagcgc gtcctggcac tgctggagga ccggccgtgg catcccgacg
       61 cgccgccgac ggccagcatc gtgccgccgc cgctgccgca accggtgctc gcgcaggccg
      121 acttcggcgt cgtccacccg acgcgcgacg cgcgccacat ggccgactgg gtggtcgcgc
      181 gccacgacaa cggccgcctg cccttcatcg tgctcgacaa gcgcgccgcg cgcctgtacg
      241 tcttccagcc caacggcacg ctggtgcagg acacgcccgt cctgctcggc cagtcgcacg
      301 gcgacgatac gtacccaggc atcggcaacg tgccgatctc cgaggtcaag ccctaccagc
      361 gcacgacggc ggccggccgg ttcgtcactc gccccggcct ggacgaggac cataccgacg
      421 tcgtctggct cgactacgac gccgcgctcg cgatgcaccg cgtcatcaac aaggtgaagt
      481 ccgagcgccg gccctggcgc ctcgcgcagc ccaacccgaa gctgcgacgc atctcgtggg
      541 gctgcatcaa catcccgatc ccgttcttca accagtacat ctcgccggtc ttcggcaagc
      601 agcgcggcgt ggtctacgtg atccccgaga ccaagacctt cgccgaggtg ttcgagaaga
      661 acggcggcgc cgcggccgtc gggcagacgc tggcgatgga caacgcgccc gcggcgccga
      721 aggccgtggt cgaccggtga gcacccgcaa aggcggctcg tgagcgcccc ggcccacgcg
      781 atcgccgcgc gccaccctcg tcggtccgcc gcgcgtgcgt gcctgtcgca tccgcgcgcg
      841 ttcatctggc gcgtgctggt ggccttcaag gccaaccagg gcctgctgct ggccggcgcg
      901 gtggcgtact acgcgctgct gtccatcgtg ccgctgctga tcctgagcgt gatcgcgctc
      961 gcgcacgtga ttccggagca cgagctgctc gagacgatcg gccgctacct cgcatggctg
     1021 gtgcccagcc agtcgggcgc ggtcgtcgcc gaactgcgcg gtttcctcga ccaccgcgag
     1081 gtcgtcggct ggttcctcgc cgtgacgatg ctgttcttca gctccctcgc cttcgccgtg
     1141 ctggagaacg cgctgtccgt cgtcttcgtc caccgcgtgt cccaccgcaa gcgcacgctg
     1201 ctcgcgcgcc tgctgatgcc ctacgtctat gtgttgtgcc tcgggttcgg gctgatgatg
     1261 gtcacgctcg tggccggcgg gctcgaggag ctcggacagc gcagcatcga cttcctcggc
     1321 caccactggt cgctcggctt cctgtcgcgg ctgctgctgt acctgctggg cctgtcgggc
     1381 gagatcgtgc tgctcacctc gatctacgtc gtgatgcccg agagcccgct cgacgtgcgc
     1441 catgcgctca tcggcgcggt ctcggcggtg gcgttgtggg aggtcacgcg ccacgtgctg
     1501 gtgtggtact tcgcgacgct gtcgcaggtg ggcgtcgtct acggctcgat gacgaccgcc
     1561 atcgtcgtgc tgctgagcct ggagctgggc gcgacgctcg tgctgctggg cgcgcaggtc
     1621 atcgccgagt acgagcgcct cgagcgccac gcgccgccgg ccagggagcc cgagctcgtc
     1681 cagcgccgct gaggcgccgc gcgcgcacgg tcgggcttgc ggtaatcctt accgatgagc
     1741 agggcggcgc acgctcgcgt tttcccctgt gtccatcgtc gattcccgca tcccgccgag
     1801 ggacgccgcc tcggcagggg cccgctgccg ttgtaggaat cgtctggcat ggaacctgca
     1861 cagacggcag agctggccga gtggacgccg atttttcccg ggcgccccgc gagccgcgcg
     1921 cctggagacg actcatgacg atcatggaaa cgcggcccgc cctggagccg gtgagcgaga
     1981 tgcaccagtt gctgggagcg ctcgtcgcgt tgaggaaggg ggacgcggac gtgcgcctgc
     2041 cggtgcactg gtcgggtgtc gcggggcgcg tggccgacgc attcaacgac gtcgtcgagc
     2101 agaacgccaa catggccgag gagctcgccc gcctgcgcca gatggtcggc aaggaaggca
     2161 agctcaagca gcgggcgtcg ctgcgcgagg ccaagggctt ctggggcgag tcgatcgact
     2221 gcatcaactc gctgatcgac gacctcgtgc acccgaccag cgaggtggcg cgcgtgatcg
     2281 gcgcggtggc ccagggcgac ctgagcaaga gcatggcgct ggaggtcgac ggccggccgc
     2341 tggagggcga gttcctgcgc acggccaaga cgatcaacaa gatggtcgac cagctcggca
     2401 atttctcggc cgaggtcacg cgggtggcgc gcgaggtcgg caccgagggc aagctcggcg
     2461 gccaggccaa ggtcaagggc gtggccggca cgtggaagga cctgacggac tcggtcaact
     2521 cgatggccgg caacctgacc gaccaggtgc gcaacatcgc cgacgtgacg accgcggtcg
     2581 cgaagggcga cctgtccaag aagatcgacg tcgacgtgaa gggcgagttc ctcacgctga
     2641 agaacacgat caacacgatg gtcgaccagc tgcgatcctt cgcgtcggag gtgacgcggg
     2701 tggcgcgcga ggtcggcacc gagggctcgc tgggcggcca ggcgcgcgtc gagggcgtgt
     2761 cggggacgtg gaaggacctg accgactcgg tcaactcgat ggccgggaac ctgacctcgc
     2821 aggtgcgcaa catcgccgac gtgacgaagg ccgtggccgc gggcgacctg tccaagaaga
     2881 tcacggtcga cgtcaagggc gagatcctcg agctgaagaa caccgtcaac acgatggtcg
     2941 accagctgcg ctcgttcgcg gccgaagtga cgcgcgtggc gcgcgaggtc ggcaccgagg
     3001 gcaagctggg cggccaggcg gacgtgcagg gcgtggcggg cacgtggaag gacctgaccg
     3061 actcggtcaa ctcgatggcc ttcaacctga cctcgcaggt gcgcaatatc gccgacgtga
     3121 ccaaggcggt ggcggcgggc gacctgtcca agaagatcac ggtggacgtc aagggcgaga
     3181 tctccgagct caagaccacc atcaacacga tggtggacca gctgtcgtcc ttcgccgcgg
     3241 aagtgacgcg ggtggcgcgc gaggtgggca ccgagggccg gctgggcggc caggccgagg
     3301 tcaagggcgt ttcgggcacc tggaaggacc tgaccgactc ggtcaactcg atggccggca
     3361 acctcacctc gcaggtgcgc aacatcgccg acgtgacgaa agccgtggcg gcgggcgacc
     3421 tgtccaagaa gatcacggtg gacgtcaagg gcgagatcct cgagctgaag aacacgatca
     3481 acacgatggt cgaccagctg cgctcgttcg cggccgaggt cacgcgcgtg gcgcgcgagg
     3541 tcggcacgga aggcaagctg ggcggccagg cggacgtgca gggcgtagcg ggcacgtgga
     3601 aggacctgac cgagtctgtg aacttcatgg ccggcaacct gacctcgcag gtgcgcaaca
     3661 tcgccgaggt gacgaccgcc gtggccgcgg gcgacctctc gaagaagatc accgtcgacg
     3721 tcaagggcga gatcctcgag ctcaagaaca cgatcaacac gatggtcgac cagctgcgct
     3781 cgttcgcggc cgaggtcacg cgcgtggcgc gcgaggtcgg caccgagggc aagctgggcg
     3841 gccaggccga cgtgcagggc gtggcgggca cgtggaagga tctcaccgag tcggtgaact
     3901 tcatggccgg caacctgacc tcgcaggtgc gcaacatcgc cgaggtgacg acggccgtgg
     3961 ccgcgggcga cctctcgaag aagatcacgg tggacgtgaa aggcgagatc tccgagctca
     4021 agaacacgat caacacgatg gtcgaccagc tgtcgtcctt cgccgcggaa gtgacgcggg
     4081 tggcgcgcga ggtcggcacc gagggcaagc tcggcggcca ggccgacgtc cagggcgtcg
     4141 cgggaacgtg gaaggacctg accgagtcgg tgaactcgat ggcgtccaac ctgacctcgc
     4201 aggtgcgcaa catcgccgac gtgacgaagg ccgtggccgc gggcgacctg tccaagaaga
     4261 tcagcgtgga cgtccagggc gagatctccg agctcaagag cacgatcaac acgatggtgg
     4321 atcagctgcg ctcgttcgcg gccgaggtca cgcgcgtggc gcgcgaggtc ggcaccgagg
     4381 gcatcctggg cggccaggcg gacgtgcagg gcgtggcggg cacgtggaag gacctgaccg
     4441 agtcggtgaa cttcatggcc ggcaacctga cctcgcaggt gcgcaacatc gccgaggtca
     4501 cgacggccgt ggcggcgggc gacctctcga agaagatcac ggtggacgtc aagggcgaga
     4561 tctcggagct caagaacacg atcaacacga tggtcgacca gctgcggtcg ttcgcggccg
     4621 aggtcacgcg cgtggcgcgc gaggtcggca ccgaaggcaa gctgggcggc caggcggacg
     4681 tgcagggcgt tgccggcacg tggaaggacc tcaccgattc ggtgaacctg atggcgtcga
     4741 acctgacctc gcaggtgcgc aacatcgcgg acgtgaccaa ggcggtggcc gcgggcgacc
     4801 tctccaagaa gatcaccgtc gacgtcaagg gcgagatcct ggagctgaag gcgaccatca
     4861 acacgatggt ggaccagctg tcgtccttcg ccgcggaagt gacgcgggtg gcgcgcgagg
     4921 tgggcaccga gggccggctg ggcggccagg cgcaggtgca gggcgtctcg ggcacgtgga
     4981 aggatctcac cgacaacgtg aacttcatgg ccggcaacct gacctcgcag gtgcgaggca
     5041 tcgccaaggt cgtgaccgcc gtggccgacg gcgacctcga gcgcaagctg acggtggagg
     5101 ccaagggcga gatcgccgcg ctggccgaca cgatcaacgg catgaccgac acgctcgcga
     5161 ccttcgccga ccaggtgacg acggtggcgc gcgaggtggg cgtcgagggc aagctcggcg
     5221 gccaggccaa ggtgcccggc gcgtcgggaa cgtggaaggc gctgaccgag aacgtcaacg
     5281 agctcgccgc caacctcacg acgcaggtgc gcgccatcgc ggaggtcgcg accgccgtca
     5341 cccagggcga cctgacgcgc tcgatcacgg tcgagactca gggcgaggtc gcctcgctga
     5401 aggacacgat caacgagatg atccgcaacc tgcgcgacac gacgcagaag aacaccgagc
     5461 aggactggct caagaccaac ctcgccaagt tcagccgcat gctgcagggg cagcgcgacc
     5521 tggtggcggt gggccagctg atcctgtccg agctcgcgcc cgtcgtcggc gcgcagcagg
     5581 ccgagttcta cgtgatgggc agctccgcgg aggcgcccaa gctgcgcctg atcgccagct
     5641 acgcctcggg cggccaggcg acgcacggca aggaggtgca cctcggcgaa ggcctggtcg
     5701 gccagtgcgc cgtcgacaag cgcaagctgc tgctgaccaa cgtgccgtcg accgccttcc
     5761 gcatcgcatc cggcttgagc gagagcacgg cgatggacgt gctggtgctg cccgtcgtct
     5821 tcgaagggca gatgcgaggc gtcctggagc tcgcctcgct ggagcgcttc aaccccgtgc
     5881 acgaggcctt cctcgaccag ctgaccgagt cgatcggcat cgtcatcaac acgatcgagg
     5941 cgaactcgcg caccgaggac ctgctcaagc agtcccagtc gctcgccgag gagctgggtt
     6001 cgcgccagaa ggagctgcag cagaccaacc aggagctgca ggacaaggcg cgcctgctgg
     6061 cgcaccagaa ccaggaggtc gagcgcaaga acaccgaggt cgagcaggcg cgccaggcgc
     6121 tcgaggagaa ggccgagcag ctcgcgttga cgtccaagta caagtccgag ttcctcgcca
     6181 acatgtcgca cgagctgcgc acgccgctca acagcctgct gatcctgtcc gaccagctgt
     6241 gcaagaacgc ggagggcaac ctgacgccca agcaggtcga gttctcgaag acgatccact
     6301 cgtccggcaa cgacctgctg atgctgatca acgacatcct ggacctgtcg aagatcgagt
     6361 cgggcacggt ggcggtggat gcgagcgagc tgcgcgtgga cgacctgcac cgctcggtgg
     6421 agcgcggctt ccgccacttc gcggagtcca agcacgtcga cttcgtcgtg gagctgcgcc
     6481 agccgctgcc caagacgatg gtcacggacg tcaagcgcct gcagcagatc atcaagaacc
     6541 tgctgtcgaa cgccttcaag ttcacgcacg aaggccaggt gcgcttcacg attgccaccg
     6601 cgacgggcgg ctggtcggcg gacaacgagg agctcgcgcg cgcggcgcag gtgttggcgt
     6661 tctcggtggc cgatacgggt atcggcatct cgcccgacaa gcagcagatc atcttcgagg
     6721 cgttccagca ggccgacggc tcgacctcgc gcaagtacgg cggcacgggc ctggggctgg
     6781 cgatcagccg cgagctctcg cgcctgctgg gcggcgagat caggctgcag agcgagccgg
     6841 gccggggcag cgagttcacg ctgtacctgc cgcaggccta cagcccggcg cgcacggcgc
     6901 ggcacgtccg g
//
LOCUS       DASVLN010000051         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_164139, whole genome shotgun sequence.
ACCESSION   DASVLN010000051 DASVLN010000000
VERSION     DASVLN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164139"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="VF453_01145"
     CDS             <1..1659
                     /locus_tag="VF453_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX7686273.1"
                     /translation="ARRATHAGQAVREATVDGRMPARFESASWVVRSALCVEVRDPRR
                     ANGPQAERSGNASGVLYVFMPPLERLEDYLELLAAIEATAAELGVRLVLEGYPPPRDP
                     RLKVLPVTPDPGVIEVNIHPAANWRELVEHTEFLYDAAWRSRLTTEKFMIDGRHTGTG
                     GGNHFVLGGATPADSPFLRRPDVLGSLLAYWHDHPSLSYLFSGLFIGPTSQAPRIDEA
                     RNDQLYELEIALAEIAKATRERGEAVPPWLVDRALRNILVDATGNTHRSEFCIDKLYS
                     PDSSTGRLGLLELRAFEMPPHARMSVVQQLLLRALVARFWARPYDGALVRWGTELHDR
                     FLLPHFVAADFDDVIGEMAEAGMPVDRAWFDPHFEFRFPKLGEFAAGGVHVVLRGALE
                     PWHVMGEEGSAGGTVRYVDSSLERLEVKVTGFVTPRHVLTVNGQPVPLQPTGRVGEYV
                     AGVRYRAWRPPSGLHPTIPVHSPLTVDLVDTWSRRSLGGCTYHVAHPGGRNYATLPVN
                     AYEAESRRLARFFAMGHTPGRMDVGQPRRSDEFPFTLDLRRAGR"
     gene            1853..>3164
                     /locus_tag="VF453_01150"
     CDS             1853..>3164
                     /locus_tag="VF453_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX7686274.1"
                     /translation="MSSVQTSLLPDDAPEAAALARAAALRGTPGSFDELHGTRAEEAG
                     HDLAPAWRGFFDAAGADGWVGLAQTRARIARRVQDDGVTYNVYADATGRASAWPLEPL
                     PFLLDAASWAAIEAGAAQRARLLDAVLADVYGSRSLLDEGLLPASLVLAHPQYLRALH
                     GCRPAGGVRLHVAAFDLARGADGAWRVVSQRTQAPSGLGYLLENRLIISQQFPAAFRD
                     LHVQRVAAGFLGLLQGLQRLHPAGEDSRVVLLTPGPHNETYFEHAFLARYLGITLVEG
                     GDLTVRGERLYLKTLHGLEPVHVLLRRVDDEYLDPLELRGDSALGVAGLLSVIRAGQV
                     VVANAPGAGFLESPGLAAFWPAVARRLLGEELLLPSTTSWWCGEDSVWQAQRDRLADF
                     VVAPTFPTSATTTGFAPHGVAELAPEARAALLAAIEARPAAYTLQ"
BASE COUNT          377 a         1212 c         1120 g          455 t
ORIGIN      
        1 gcgcgccgcg cgacgcacgc cggccaggcg gtgcgcgagg ccacggtcga cggccgcatg
       61 ccggcgcgct tcgagtcggc gagctgggtc gtgcgctcgg ccctgtgcgt cgaggtgcgc
      121 gacccgaggc gcgccaacgg cccgcaggcg gagcgtagcg gcaatgccag cggcgtgctc
      181 tacgtcttca tgccgccgct ggaacggctg gaagactacc tcgagctgct ggccgcgatc
      241 gaggccacgg ccgccgagct gggcgtccgc cttgtgctcg agggctatcc gccgccccgc
      301 gatccgcgcc tgaaggtgct gcccgtgacg cccgaccccg gcgtgatcga ggtcaacatc
      361 catccggccg cgaactggcg cgagctcgtg gagcacaccg agttcctgta cgacgccgcc
      421 tggcgctcgc gcctgacgac cgagaagttc atgatcgacg gccgccacac cggcaccggc
      481 ggcggcaacc acttcgtcct cggcggcgcg acgccggcgg actcgccctt cctgcgccgg
      541 cccgacgtcc tcggcagcct gctggcctac tggcacgacc atccgtccct gagctacctg
      601 ttctccggcc tgttcatcgg gcccaccagc caggcgccgc gcatcgacga ggcgcgcaac
      661 gaccagctct acgaactcga gatcgccctg gcggagatcg cgaaggcgac gcgcgagcgc
      721 ggcgaggccg tgccgccctg gctcgtggac cgcgcactgc gcaacatcct cgtcgatgcc
      781 acgggcaaca cgcaccgcag cgagttctgc atcgacaagc tgtactcgcc cgactcgtcg
      841 accgggcggc tggggctgct cgagttgcgc gccttcgaga tgccgcccca cgcgcgcatg
      901 agcgtcgtcc agcaactgct gctgcgggcg ctcgtggcgc gcttctgggc ccggccctac
      961 gacggcgcgc tggtgcgctg ggggacggag ctccacgacc gcttcctgct gccgcacttc
     1021 gtggccgccg acttcgacga cgtcatcggc gagatggccg aggccggcat gccggtcgac
     1081 cgcgcctggt tcgacccgca cttcgagttc cgcttcccca agctgggcga gttcgcggcc
     1141 ggcggcgtgc acgtggtgct gcggggcgcg ctggagccgt ggcacgtgat gggcgaggag
     1201 ggcagcgccg gcggcacggt gcgctacgtc gactcgtcgc tggagcggct ggaggtcaag
     1261 gtcaccggct tcgtcacgcc gcgccacgtc ctgaccgtca acggccagcc ggtgcctctg
     1321 cagccgaccg ggcgcgtcgg cgaatacgtc gccggcgtgc gatatcgcgc gtggcggccg
     1381 ccctccggcc tgcatccgac gatcccggtt cactcgccgc tgaccgtcga cctcgtcgat
     1441 acctggtcgc ggcgctcgct gggcggctgc acgtaccacg tcgcgcatcc aggcggccgc
     1501 aactacgcga cgctgcccgt caatgcctac gaggccgaga gccgccgcct cgcgcgcttc
     1561 tttgcgatgg gccacacgcc gggccggatg gacgtggggc agccccggcg cagcgacgag
     1621 ttcccgttca cgctggacct gcgacgcgcg gggcgctgac gatgatcgcc ccgccacgga
     1681 tggacgcatt ggcgccatga ctgcgttcgc atcgaccttc gcgtccacgt ccaggtgcgc
     1741 gctcttgccg gcgtccggat gcgcgctgtg gccgaccttc caggaggcgc tgcggcccgc
     1801 gggcccggcg atgccgggct cctggcgata atccgtgctc atcccctccc cgatgtcctc
     1861 cgtccagacc agcctgcttc ccgacgatgc gcccgaggct gccgccctcg cgcgcgcggc
     1921 ggcgctgcgt ggcacgccgg ggagcttcga cgagctgcac ggcacccgcg ccgaggaggc
     1981 cggccacgac ctggcgccgg cctggcgcgg tttcttcgac gcggccggcg ccgacggctg
     2041 ggtcggcctc gcgcagacgc gcgcgcgcat cgcgcggcgc gtccaggacg atggcgtcac
     2101 ctacaacgtc tatgccgacg ccacgggcag ggccagcgcg tggccgctcg agccgctgcc
     2161 tttcctgctc gatgccgcgt cctgggctgc gatcgaggcc ggcgccgccc agcgcgcacg
     2221 cctgctcgat gcggtgctcg ccgacgtcta cggcagccgc agcctgctgg acgaggggct
     2281 gctgcccgcc tcgctggtgc tcgcacatcc gcagtacctg cgcgcgctgc acggctgccg
     2341 cccggcgggc ggtgtccggc tgcatgtcgc ggccttcgac ctcgcgcgcg gcgcggacgg
     2401 cgcctggcgc gtcgtcagcc agcgcaccca ggcgccgtcc gggctcggct acctgctgga
     2461 aaaccggctc atcatctcgc agcagttccc cgccgccttt cgcgacctgc acgtgcagcg
     2521 cgtggccgcg ggcttcctcg ggttgctgca gggcctgcag cgcctgcatc ccgcgggcga
     2581 ggattcgcgc gtcgtgctcc tgacgcccgg gccccacaac gagacctact tcgagcacgc
     2641 gttcctcgcg cgctacctcg gcatcacgct ggtcgagggc ggcgacctca ccgtgcgcgg
     2701 cgagcgcctc tacctgaaga cgctgcacgg cctggagccg gtgcacgtgc tgctgcggcg
     2761 cgtcgacgac gagtacctcg acccgctgga gctgcgcggc gactcggcgc tgggcgtcgc
     2821 cgggctgctg tcggtgatcc gcgcggggca ggtcgtcgtg gccaacgcgc cgggcgccgg
     2881 cttcctcgag agtcccggcc tcgccgcgtt ctggcccgcg gtcgcgcgcc ggctgctggg
     2941 cgaggagctg ctgctgccct cgacgaccag ctggtggtgc ggcgaggaca gcgtctggca
     3001 ggcccagcgc gaccgcctgg ccgacttcgt cgtcgccccc accttcccga ccagcgccac
     3061 gacgaccggc ttcgcgcccc acggcgtcgc cgaactcgcg cccgaggcgc gtgccgcgct
     3121 gctggccgcg atcgaggcgc ggcccgcggc gtacacgctg cagg
//
LOCUS       DASVLN010000052         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_378741, whole genome shotgun sequence.
ACCESSION   DASVLN010000052 DASVLN010000000
VERSION     DASVLN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378741"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..691)
                     /gene="orn"
                     /locus_tag="VF453_01155"
     CDS             complement(131..691)
                     /gene="orn"
                     /locus_tag="VF453_01155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX7686275.1"
                     /translation="MTETLLKKSEMNLIWVDMEMTGLLPDSDRIIEVAVVVTSPDLAV
                     RVEGPVFAIHQSDAVLDGMDAWNKGTHGKSGLIDRVRASTVTEAQAEAELVKWLADYV
                     PRGKSPMCGNSICQDRRFMARTMPALEEYFHYRNLDVSTLKELARRWKPAALEGLKKA
                     QKHTALADIQESIDELVHYRSTLLAV"
     gene            874..>2187
                     /locus_tag="VF453_01160"
     CDS             874..>2187
                     /locus_tag="VF453_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7686276.1"
                     /translation="MNPLVLSTAFALAILASLAVKYWLATRQMRHVAAHRDAVPPAYA
                     GVVTPEAHRKAADYSLAKLRLGIVSDAVGAAVLIGWTLLGGLAWLNQAVLGLVEPRWG
                     AMAYQLALFGAFSVVGWLVDLPIALYSTFALEQRFGFNRMTAGRYVKDTLLSTALGVA
                     IGGPLLAAVLWIMQAAGTHWWVLAWAVLVAFQLLAMVVYPAFIAPLFNRFEPLADEAL
                     AARVQALMARCGFRARGFYVMDGSKRSAHSNAYFTGFGASKRVVFYDTLLARLSGDEL
                     EAVLAHELGHFAHRHILKRLLAVFAFSLAGFALLGWLANQPAFYYGLGTLPNMSAPNN
                     AQALLLFMLALPPFLYFVSPLFASLSRRDEYQADAYARDRASATELVRALLKLYEDNA
                     TTLTPDPVFVRFYYSHPPASARIAALGVTIAALAGVAAGATAPEPA"
BASE COUNT          267 a          786 c          756 g          378 t
ORIGIN      
        1 cccgttcgag gtcacgcccg cgatgttcgc gtggatggag caccgctacg agccgccggc
       61 cgccgacgag ctcgcgggcg cggtcgtggt cgacacgtcg cccgggcagg ccgctgccgg
      121 ctgaaggacg tcagaccgcc aacagggtgc ttcggtagtg caccagctcg tcgatcgatt
      181 cctggatatc ggcgagcgcg gtgtgcttct gcgccttctt caggccctcg agggctgccg
      241 gcttccagcg ccgcgcgagc tccttgagcg tgctgacgtc gaggttgcgg tagtggaagt
      301 actcctcgag cgccggcatc gtgcgcgcca tgaagcggcg atcctggcag atcgagttgc
      361 cgcacatcgg gctcttgccc cgcggcacgt agtcggcgag ccacttgacg agctcggcct
      421 cggcctgcgc ctcggtgacg gtcgacgcgc gcacgcggtc gatcaggccg ctcttgccgt
      481 gggtgccctt gttccaggcg tccatgccgt cgagcacggc gtcgctctgg tggatcgcga
      541 agacgggacc ttcgacgcgc accgcgagat cggggctggt gacgaccacg gcgacctcga
      601 tgatgcggtc gctgtcgggc agcaggccgg tcatctccat gtcgacccag atcaggttca
      661 tctcggattt cttcagcagc gtctcggtca tcgggtctcc caggtgcgtg gcaggacggg
      721 atcgggcgtc gccgcgaacg cgcaccgatc cttgaacgac ggcattctcg ccccgatctg
      781 cggcgggccc gccgtcgagc gcgggcgaaa tgcggcattt ccgggccgat ccgacaccgg
      841 cgcccgcggc cgcgccgcct acacttgagc cccatgaatc ctctcgtcct gtccacggcg
      901 ttcgcgctgg ccatcctcgc ctcgctggcc gtgaagtact ggctcgccac gcgccagatg
      961 cgccacgtcg ccgcgcaccg cgacgcggtc ccgcctgcct acgccggcgt ggtcacgccc
     1021 gaggcgcatc gcaaggccgc ggactactcg ctcgcgaagc tgcggctggg catcgtgtcc
     1081 gacgccgtcg gggcggccgt gctgatcggc tggacgctgc tgggcggact cgcgtggctc
     1141 aaccaggcgg tgctcgggct ggtcgagccg cgctggggcg cgatggccta ccagctcgcg
     1201 ctgttcggtg cgttcagcgt ggtcggttgg ctggtcgacc ttccgattgc cctgtactcg
     1261 accttcgcgc tggagcagcg cttcggcttc aaccgcatga cggcgggccg ctacgtcaag
     1321 gacacgctgc tgtcgacggc gctgggcgtc gcgatcggcg ggccgctgct tgccgcggtg
     1381 ctgtggatca tgcaggccgc cggcacgcac tggtgggtgc tcgcgtgggc ggtgctcgtc
     1441 gcgttccagc tgctggcgat ggtggtctac ccggccttca tcgcgccgct tttcaaccgg
     1501 ttcgagccgc tcgccgacga ggccctggcc gcgcgggtgc aagcgctgat ggcgcgctgc
     1561 ggcttccgcg cccgcggctt ctacgtcatg gacggcagca agcgctcggc gcactcgaac
     1621 gcctacttca cgggcttcgg cgcgtccaag cgggtggtgt tctacgacac gctgctcgcg
     1681 cgcctgagcg gtgacgagct cgaggccgtg ctggcgcacg agctgggtca tttcgcgcac
     1741 cgccacatcc tcaagcgcct gctggcggtg ttcgcgttca gcctcgccgg cttcgcgctg
     1801 ctgggctggc tcgcgaacca gccggcgttc tactacggcc tgggcacgct gcccaacatg
     1861 agcgcgccca acaacgccca ggcgctgctg ctgttcatgc tggcgctgcc gccgttcctg
     1921 tacttcgtct cgccgctgtt cgcgagcctg tcgcgccgcg acgagtacca ggccgacgcc
     1981 tacgcccgcg atcgcgcgag cgcgacggag ctcgtgcgcg cgctgctcaa gctctacgag
     2041 gacaacgcga cgacactcac gccggacccg gtcttcgtgc gcttctacta ctcgcacccg
     2101 ccagcctcgg cgcgcatcgc ggcgctgggc gtgaccatcg ccgcgctggc gggcgtcgcc
     2161 gccggcgcga ccgcccccga gcccgcc
//
LOCUS       DASVLN010000053         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_378830, whole genome shotgun sequence.
ACCESSION   DASVLN010000053 DASVLN010000000
VERSION     DASVLN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378830"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VF453_01165"
     CDS             complement(<1..866)
                     /locus_tag="VF453_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX7686277.1"
                     /translation="MKTVHHALVPSTPGVRAELVSLHYGTPGQGPKATLQAALHADEI
                     PGLLVAHHLRERLAALEAAGRIRGEVVLVPVANPLGLGQRVLQSVQGRFDLATGENFN
                     RHYADLLEDVARAVDGRLGADADRNVATIREALRAAVAAVPERTALESLRRTLLSLAV
                     DADIVLDLHCDSEAALHLYTETSVWPRVEPLARLMGARAVLLAEESGDHPFDEACSTL
                     WPRLAAQFGAATPIPSACVAVTVELRGEADVSHELAARDADALVDYLCWQGVIAGGPT
                     SLPPASCEATPLA"
     gene            complement(1073..1723)
                     /locus_tag="VF453_01170"
     CDS             complement(1073..1723)
                     /locus_tag="VF453_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7686278.1"
                     /translation="MELSPPDTSVLRIEKLVFAYGRRWLFEGFSAEARPGVTWVQGAN
                     GSGKSTLLRLLAGASRPQHGRFALGGEHYGCGDRVAPLAWRRQTFWGLSDPPPTPWLT
                     IGECLGFVAGTYGLPAQGSQALREQVEALGLERVLETPLRHASLGQQRKTLLALALAL
                     PVRLLLLDEPFNALDAASAAHLARVLGERAEAGRQVVLLTSHVEPHVAVRDRWVLG"
     gene            complement(1714..>1989)
                     /locus_tag="VF453_01175"
     CDS             complement(1714..>1989)
                     /locus_tag="VF453_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686279.1"
                     /translation="WLAALPVADGWRRRARAIACAPMLLAGAAAVALVALAPRPFRTV
                     PLAAFAVGLVATPVALTAVHSSRRETHVSLWALCTGLLTAVGSELWS"
BASE COUNT          271 a          750 c          756 g          212 t
ORIGIN      
        1 gccagcggcg tggcctcgca gctcgcaggc ggcagcgacg tcggcccgcc ggcgatcacg
       61 ccctgccagc acaggtagtc gacgagcgcg tcggcgtcgc gcgcggccag ctcgtgcgag
      121 acgtcggcct cgccgcgcag ctccacggtc accgcgacgc aggccgaggg gatcggcgtc
      181 gcggcgccga attgcgcggc caggcgcggc cagagcgtcg agcaggcctc gtcgaacggg
      241 tggtcgcccg attcctcggc cagcagcacc gcgcgcgcgc ccatcaggcg ggccagcggc
      301 tcgacgcgcg gccagaccga ggtctcggtg tacaggtgca gcgcggcctc gctgtcgcag
      361 tgcaggtcca gcacgatgtc ggcatcgacc gccagcgaca gcagcgtgcg gcgcaggctc
      421 tcgagcgcgg tgcgttccgg caccgcggcg accgcggcgc gcagggcctc gcggatggtc
      481 gcgacgttgc ggtccgcgtc ggcgccgagg cggccgtcca cggcgcgcgc gacgtcctcg
      541 agcaggtcgg cgtagtggcg gttgaagttc tcgccggtcg cgagatcgaa gcggccctgc
      601 accgactgca gcacgcgctg tccgagcccc agcggattgg ccaccggcac gagcaccacc
      661 tcgccgcgga tgcggccggc ggcctcgagc gcggccaggc gctcgcgcag gtggtgggcg
      721 acgagcaggc ccgggatctc gtcggcgtgc agcgcggcct gcagcgtcgc cttcggcccc
      781 tggcccggcg tgccgtagtg caggctgacc agttcggcgc ggacgccggg cgtggacggg
      841 acgagggcgt ggtggacggt cttcatcgcg ggaatccttc aggaagtgcg ggcctgccca
      901 gggtggcggc cagcggcgtt cggcggcaag gaaccggcgc gccggcgcct tcgagatcgc
      961 gagggacgcg accgccggca tgaggcatgg cgcggcggcg gtcgggcggc aaggcgccga
     1021 ggttaccgca cgccccgcgt ggggagtcgc ttgcccggcc gcatcgcgaa gctcacccca
     1081 gcacccaccg gtccctcacc gcgacatgcg gctcgacatg actggtcagc aggaccacct
     1141 gccgcccggc ttcggcccgc tcgcccagca cccgggcgag atgcgccgcc gacgccgcat
     1201 ccagcgcgtt gaagggctcg tcgagcagca gcaggcgcac gggcaacgcc agcgccaggg
     1261 ccagcagcgt cttgcgctgt tgcccgagcg acgcatgccg cagcggcgtc tcgagcaccc
     1321 gctccaggcc cagcgcctcc acctgctcgc gcagcgcctg cgacccctgc gcgggcaggc
     1381 cgtaggtgcc ggcgacgaag ccgaggcatt cgccgatggt gagccacggc gtcggcggcg
     1441 ggtcgctcag gccccagaag gtctggcggc gccaggccag cggcgcgacg cggtcgccgc
     1501 agccgtagtg ctcgccgccc agcgcgaagc ggccgtgctg cggccgcgac gcccccgcga
     1561 gcaggcgcag cagcgtcgac ttgccgctgc cgttggcgcc ctgcacccag gtgacgcccg
     1621 ggcgggcctc ggcgctgaag ccctcgaaca gccagcgccg gccataggcg aagaccagct
     1681 tctcgatgcg caggacggac gtgtccggcg gactcagctc cacagttcgc tccccacggc
     1741 cgtcagcagg ccggtgcaca aggcccagag gctgacatgg gtctcgcgcc ggctcgaatg
     1801 cacggcggtc agcgcgacgg gcgtcgcgac gagtccgacc gcgaacgccg ccagcgggac
     1861 cgtccggaac ggccgcggcg cgagcgcgac gagcgcgacc gccgccgcgc cggccagcag
     1921 catcggcgcg caggcgatcg cccgagcgcg ccgccgccag ccgtcggcca ccggcagcgc
     1981 cgcgagcca
//
LOCUS       DASVLN010000054         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_56338, whole genome shotgun sequence.
ACCESSION   DASVLN010000054 DASVLN010000000
VERSION     DASVLN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56338"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VF453_01180"
     CDS             complement(<1..1044)
                     /locus_tag="VF453_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686280.1"
                     /translation="MSAAPWLVITIRHPDKVTENFADQLVAGLAAHGRRAYVAVHGPE
                     LGAQLAAVPLAELGGVIAIGPLPLGIQVGGQPLYRRLACPFHFYILDTLLYDIARFPV
                     AGQFLADAWSDPRLVPVFAEKATRDAWCTGPAPLAPPHSLYVPFAAFPEPPGPRPVVP
                     VQRRLVVVGSIGTELAAGIVQPDLARTLAESNAMALSGNELARVAERLLAPGGRSNTV
                     LDLFDVLALSPRAALEPEVQKFACAADSFVKRHRRVLAAQSLRGVPVDFVGSGWQQVF
                     GDEPSFRFLGNLPHGSLTRLMTFYAGLVNFDPNWEWGMHDRVYSALSVGTPVLTNANQ
                     APADEGLPAALVAT"
     gene            complement(1226..2215)
                     /locus_tag="VF453_01185"
     CDS             complement(1226..2215)
                     /locus_tag="VF453_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686281.1"
                     /translation="MTRFLFVSPDSNTPTGGVKVIYKHAELLSRLGHDAHVMHFQRPY
                     RCDWFEHQAPIVYFDELRTTDMLLVPEIMTIFGNQLANIGMRYCMFVQNGYLVLPTAS
                     LPDVWNCYKNATAILSISDDTSAFLASVFPEFAHKIIRVKYSVDTERFKPRAKLKKCT
                     FMPRKLPLHSANLVPWLARAFPDWTFQPIHGMNEAQVAEQLESSRVFLAFSDFEGCPV
                     PPIEAALSGNVVVGYPGWGGREYWNAPNFLPVPVGDMRAFVEQFRPAAGFSLRDDAAQ
                     LLAPGIDRLKAAYGLQAETELLVRAIDRVKEFSAAQGPVQPRKAPAPVAVAAA"
     gene            complement(2227..3156)
                     /locus_tag="VF453_01190"
     CDS             complement(2227..3156)
                     /locus_tag="VF453_01190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7686282.1"
                     /translation="MPANDLVSVLIPAFKPGHFRAALESVLAQEHGALEVIVGDDSPG
                     AGIRAIVEELADPRVAYMPSRAVTGGDPKLNHVLLWHKARGRYVRFMYDDDLIAPTST
                     GTLLGLLRAQPGCALSWHQRRLIDEHGRVTQQAGPLKPGQAAVMDRALLLDNMAKFLN
                     FVGEPSFTMFDRERVPHFGFNRYGRWDTAFLWDVAMYLAATEHGLAHGSGDFLGDFRV
                     HSGQVSSRANVFGAVEWELVFREELRAGRVSVEQFHVMLPKLLGLYQASKHTLPALHR
                     FQSQLLADAQAGRLRESTPEFLAHYEPLLAEHR"
BASE COUNT          495 a         1090 c         1297 g          445 t
ORIGIN      
        1 ggtggcgacg agcgccgccg gcaggccttc gtcggccggc gcctggttgg cgttggtcag
       61 gacgggcgtg cccaccgaca gcgcgctgta gacgcggtcg tgcatgcccc attcccagtt
      121 cgggtcgaag ttgacgagcc cggcgtagaa cgtcatcagc cgcgtcaggc tgccgtgcgg
      181 caagttgccg aggaagcgga acgacggctc gtccccgaac acctgctgcc agccgctgcc
      241 gacgaagtcc accggcacgc cgcgcagcga ctgcgccgcc agcacgcgtc ggtggcgctt
      301 gacgaaggag tcggccgcgc aggcgaactt ctgcacctcg ggctcgagcg cggcgcgcgg
      361 gctcaaggcc aggacgtcga acaggtcgag cacggtgttg gatcgcccgc ccggcgcgag
      421 caagcgctcg gccacgcgcg cgagttcgtt gccggacagc gccatcgcgt tggactcggc
      481 cagcgtgcgc gcgagatcgg gctgcacgat gccggccgcc agctcggtgc cgatcgagcc
      541 gaccacgacc agccgccgct gcaccgggac gacgggacgc ggccctggcg gctccgggaa
      601 ggccgcgaac ggcacgtaca ggctgtgcgg cggcgcgagc ggcgcagggc ccgtgcacca
      661 ggcgtcgcgc gtggccttct cggcgaacac cggcacgagg cgcggatcgc tccacgcgtc
      721 ggcgaggaat tgcccggcca cggggaagcg cgcgatgtcg tagagcagcg tgtcgaggat
      781 gtagaagtgg aacgggcagg cgagtcggcg gtagagcggc tggccgccga cctggatgcc
      841 cagcggcagc ggcccgatgg cgatcacgcc gccgagttcc gccagcggca ccgcggcgag
      901 ctgcgcgccg agctcgggtc cgtgcaccgc gacgtaggcc cggcggccgt gcgcggccag
      961 cccggcgacg agctggtccg cgaagttctc cgtcaccttg tccgggtggc ggatggtgat
     1021 gacgagccac ggggccgcgc tcacgacggg cgctcctggg tcgctgcgat ccggcggacg
     1081 aaagatggag gcatgtcggc agtctgcggt cgaactgcag ccggcgatcc ctggaatcgc
     1141 ggcccgaacc ggggccagct cggcgcttcc ggcgcgaaac gcgaacggcc gcgcgaggcg
     1201 gccgtcggcg aggggcggga cgggctcagg ccgcggcgac ggcgacgggc gccggcgcct
     1261 tgcgcggctg gaccgggccc tgggcggcgg agaactcctt gacgcggtcg atggcgcgca
     1321 cgagcagctc ggtctccgcc tgcagcccgt aggcggcctt caggcggtcg atgccggggg
     1381 cgagcagctg ggcggcgtcg tcgcgcagcg agaacccggc cgcgggacgg aactgctcga
     1441 cgaacgcgcg catgtcgccc accggcaccg gcaggaagtt gggtgcgttc cagtactcgc
     1501 ggccgcccca gccggggtag ccgaccacga cgttgccgct cagcgcggcc tcgatcggcg
     1561 gcaccgggca gccctcgaag tcgctgaagg cgaggaacac gcgcgaggac tcgagctgct
     1621 cggcgacctg cgcctcgttc atgccgtgga tgggctggaa cgtccagtcg gggaaggcgc
     1681 gcgcgagcca gggcaccagg ttggcggagt gcagcggcag cttgcgcggc atgaaggtgc
     1741 acttcttcag cttggcgcgc ggcttgaagc gctcggtgtc gaccgagtac ttcacgcgga
     1801 tgatcttgtg cgcgaactcc gggaacacgc tcgcgaggaa ggccgaggtg tcgtcggaga
     1861 tggacaggat cgcggtggcg ttcttgtagc agttccagac gtcgggcagc gaggccgtcg
     1921 gcagcacgag gtagccgttc tgaacgaaca tgcagtagcg catgccgatg ttggcgagct
     1981 ggttgccgaa gatcgtcatg atctcgggca ccaacagcat gtcggtggtg cgcagctcgt
     2041 cgaagtagac gatgggcgcc tggtgctcga accagtcgca gcggtagggc cgctggaagt
     2101 gcatcacgtg ggcgtcgtgc cccaggcggc tcagcagctc ggcgtgcttg tagatcacct
     2161 tcacgccgcc ggtgggcgta ttggagtccg ggctgacgaa gaggaagcgg gtcatcgggg
     2221 cgcctttcag cggtgctcgg cgagcagcgg ttcgtagtgg gcgaggaact cgggcgtgga
     2281 ctcgcgcagg cgcccggcct gcgcgtcggc gagcagctgc gactggaagc ggtgcagcgc
     2341 gggcagggtg tgcttgctcg cctggtacag gcccagcagc ttgggcagca tcacgtggaa
     2401 ctgctcgacg ctgacgcggc cggcgcgcag ctcctcgcgg aagacgagct cccactccac
     2461 cgcgccgaag acgttggcgc gcgaggacac ctgccccgag tggacccgga agtcgcccag
     2521 gaagtcgccc gagccgtgcg cgaggccgtg ctcggtggcg gcgaggtaca tcgcgacgtc
     2581 ccacaggaac gcggtgtccc agcggccgta gcggttgaag ccgaaatgcg gcacgcgctc
     2641 tcggtcgaac atcgtgaagc tgggctcgcc gacgaagttg aggaacttcg ccatgttgtc
     2701 cagcagcagc gcgcgatcca tgacggcggc ctggcccggc ttgagcggcc cggcctgctg
     2761 cgtcacgcgg ccgtgctcgt cgatgaggcg gcgctggtgc cagctgagcg cgcagccggg
     2821 ctgcgcccgc agcagcccga ggagggtgcc ggtggacgtc ggcgcgatca ggtcgtcgtc
     2881 gtacatgaag cgcacgtagc ggccgcgcgc cttgtgccac agcagcacgt ggttgagctt
     2941 gggatcgccg ccggtgaccg cgcggctggg catgtaggcc acgcgcgggt cggccagctc
     3001 ctccacgatg gcgcggatgc cggcgccggg cgagtcgtcg cccacgatca cctcgagcgc
     3061 gccatgctcc tgcgcgagga cgctctccag cgcggcgcgg aaatggccgg gcttgaaggc
     3121 cgggatgagc acggagacca ggtcgttggc gggcatgcgg cggctagggt ggacgcggcc
     3181 gatgcgggga cggccgccgt ccggctagta caccccagcc gccggcgcgg cgatggcgcg
     3241 ataagccgtg gattgggctg cctgatcgga gccggacggg ccgtccgtgg gggcgacccg
     3301 ccgcgccgcg cggccgtctt ggcgcgc
//
LOCUS       DASVLN010000055         5089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_486432, whole genome shotgun sequence.
ACCESSION   DASVLN010000055 DASVLN010000000
VERSION     DASVLN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486432"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VF453_01195"
     CDS             complement(<1..462)
                     /locus_tag="VF453_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HEX7686283.1"
                     /translation="MSKIDVRIHGSGIVGRALALALARKGLHVGIDARPAVPAGGEAR
                     PDVRAYALNAASVALLTELKVWDGLPADARTPVYDMRIAGDADGALAFSAWQQAVPAL
                     AWIVDAAELEQVLETAIRFAPAVAVVAGDADAPLAVIAEGKAAASRERLGIA"
     gene            591..1685
                     /gene="ychF"
                     /locus_tag="VF453_01200"
     CDS             591..1685
                     /gene="ychF"
                     /locus_tag="VF453_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX7686284.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVV
                     ELPDPRLQALAAVVSPERIVPAFVEFVDIAGLVAGASKGEGLGNQFLSHIRETDAIVN
                     VVRCFEDDNVIHVAGKVDPIADIEVIQTELCLADLSTVEKSLQRYSKQARAGGDKEAQ
                     RLVEVLKKCEAALNEARPVRSIDFSKEELAVLKPLCLITAKPAMFVGNVADNGFEDNP
                     YLDRLRDYAAAQKAPVVAICAKTEADLADMEPDDQALFLAEMGQTEPGLNRLIRAAFT
                     LLGLQTYFTAGEKEVRAWTVPIGATAPQAAGVIHTDFEKGFIRAQTIAYDDYIALKGE
                     QGAKDAGKMRAEGKEYVVKDGDVLHFLFSS"
     gene            1874..3901
                     /locus_tag="VF453_01205"
     CDS             1874..3901
                     /locus_tag="VF453_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686285.1"
                     /translation="MSSSGQRSAAALARIVALATLVSLGACGGGGSSSPSPALTASAA
                     ADHATAGGQSVTLTAVASNSTDTVTWSLSGPGSLSATTGATVDYIPPGPDALDAGTQA
                     TITATLGTLTETVVLNVATAPGSNWEMTQAPSEDVAAVVQANGTYVAVGARGVAQTST
                     DGRTWTSVDTGANADLFAIAYGSAGYVAVGDFGAVVSSADGSTWTSEPRLAPSSDGFW
                     LESIAYGHGLYVVAGDHGVFTSADGVTWTTVPLFSQRMSHGGVTIGNGRFVVATTAGT
                     AWSDDGVAWTLAPSAPPGMGRLVFGNGVFVGSGTAGVATSTDGVTWSSPAVIGSTDVV
                     ALDFTHGHFLLETPTLLSTSTDGRTWTTGNNPSVIDPIQGASAFTTTPSGDYLMLGTY
                     GLAATSTDLATWTPAGQADHGDGWFWVAYANGTYLAIDDNDRAWSSTDGGSWAAGTFS
                     DPNDVNFLTAANGRFFAGGGAAGSLQTSRDGVSWTTQSTGLPNRIFGVTWGNGLYVAV
                     GDAGIATSPDGLTWTVVLTTTSPESYTAVAYGAGLFVASDNRDHIATSPDGIHWTTGA
                     TAFDSIFYGIAYGPSGFVASSLFEVWHSADGFTWTKDTLPANEVAPRKVIYAAGRYVG
                     MGSNGHVLRSTDGVTWVAQPLGLPWNMSSVAFGGDRFVAVGERGEVLVSTH"
     gene            complement(3918..4877)
                     /locus_tag="VF453_01210"
     CDS             complement(3918..4877)
                     /locus_tag="VF453_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX7686286.1"
                     /translation="MSVSSVVGIDVAKAHVDVFVLGAEFKPQRFVNDAEGHSDLATAL
                     APLGVQLVVMEATGGYEAALACALQAAGFAVAVVNPKQARDFAKGMGYLAKTDAIDAR
                     ALAAFADVLSRQPDLASYVRPLTAEAQRDLAGLVTRRRQLLAMMLSERQRLQLAVTKV
                     RPSIEAMIEHIKAQLDEVDGEMVGHVQEHFGPLDTLLRSTKGIGPVASATLIAELPEL
                     GKLNRRQIAALVGVAPMANDSGSSKGRRRVSGGRFEIRRTLYMATLTATRHNPPIAAF
                     YARLLAAGKLPKVALIACMRKLLTTLNAMARSGLAWDPEFKTA"
BASE COUNT          814 a         1771 c         1734 g          770 t
ORIGIN      
        1 cgcgatgccc aggcgctcgc ggctcgcggc ggccttgccc tcggcgatca cggccagcgg
       61 ggcgtcggcg tcgccggcga cgaccgccac ggccggcgcg aagcggatcg cggtctcgag
      121 cacctgctcc agctccgccg cgtccacgat ccaggcgagc gccggcaccg cctgctgcca
      181 ggccgagaac gcgagcgcgc cgtcggcgtc gccggcgatg cgcatgtcgt acaccggcgt
      241 gcgggcgtcc gcgggcagcc cgtcccagac cttgagctcg gtcagcagcg ccaccgaggc
      301 ggcgttgagc gcgtaggcgc ggacgtcggg gcgcgcttcg cctcccgccg gcacggcagg
      361 cctcgcgtcg atgcccacgt gcagtccctt gcgggcgagc gcgagggcca gcgcgcggcc
      421 gacgatgccg ctgccgtgga tccggacgtc gatcttggac atccggccat tgtagggagc
      481 ctggccggtg atcgcggacg gcgtggcaac ggccttaaaa tgcgcgtttc ccccgaatgc
      541 cggattcacg cgccctttcg agagccccgg ccaacccgcc aggaagcgcc atgagtctga
      601 aatgcggcat cgtcggcctg cccaacgtcg gcaagtcgac cctgttcaac gccctcacca
      661 aggccggcat cgccgccgag aactacccgt tctgcacgat cgagccgaac gtcggcgtcg
      721 tcgagctgcc cgacccgcgc ctgcaggcgc tcgcggccgt cgtctcgccg gaacggatcg
      781 tgccggcgtt cgtcgagttc gtggacatcg cgggcctcgt ggccggcgcc tccaagggcg
      841 agggcctcgg caaccagttc ctgtcgcaca tccgcgagac cgacgcgatc gtcaacgtcg
      901 tgcgctgctt cgaggacgac aacgtgatcc acgtggccgg caaggtcgac ccgatcgccg
      961 acatcgaggt gatccagacc gagctgtgcc tggccgacct gtcgacggtc gagaagtcgc
     1021 tgcagcgcta ctccaagcag gcgcgcgccg gcggcgacaa ggaggcccag cgcctcgtcg
     1081 aggtgctgaa gaagtgcgag gcggcgctga acgaggccag gccggtgcgc tcgatcgact
     1141 tcagcaagga ggaactcgcc gtcctcaagc cgctgtgcct gatcaccgcc aagccggcga
     1201 tgttcgtcgg caacgtcgcc gacaacggct tcgaggacaa cccctacctc gaccgcctgc
     1261 gcgactacgc cgccgcgcag aaggcgccgg tcgtcgcgat ctgcgccaag accgaggccg
     1321 acctcgccga catggagccc gacgaccagg ccctgttcct cgccgagatg ggccagaccg
     1381 agcccggact gaaccgcctc atccgcgccg ccttcacgct gctgggcctg cagacctact
     1441 tcacggccgg cgagaaggag gtgcgcgcgt ggacggtgcc gatcggcgcg accgcacccc
     1501 aggccgcggg cgtgatccac accgactttg agaagggctt catccgcgcc cagaccatcg
     1561 cctacgacga ctacatcgcg ctcaagggcg agcagggcgc gaaggacgcc ggcaagatgc
     1621 gcgcggaagg caaggagtac gtcgtcaagg acggcgacgt gctgcatttc ctgttctcga
     1681 gctgagcccc ccggcgcccg gaggcgcggc cctggtgcac gatgcgagcc ctcactgcgg
     1741 cgccccgtag cgaatcgtca cgatttcccc caaaagggtg catgcggacg ccgcggaggg
     1801 tgcggcatac ttcacggtcg actgccgagc cgttccgctt ccgaggaccg ccggcgcatc
     1861 cgagggagat cgcatgtcat cgtcaggtca acgcagcgcc gcggcgctcg ctcgcatcgt
     1921 cgcgctcgcg acgctcgttt cactcggcgc ctgcggcggg ggtggctcgt cgagcccctc
     1981 gcccgcgctc acggccagcg ccgcggccga ccacgcgacg gcgggcggcc agtccgtcac
     2041 cctcacggcc gtcgcctcca actccaccga cacggtcacc tggtcgctca gcggcccggg
     2101 ctcgctgagc gcgaccaccg gcgcgacggt cgactacatc ccgccgggcc cggacgccct
     2161 cgacgcgggc acgcaggcca ccatcaccgc gacgctgggc acgctgaccg agacggtcgt
     2221 gctgaacgtc gcgaccgcgc ccggcagcaa ctgggagatg acgcaggcgc cctccgagga
     2281 cgtcgcggcg gtggtgcagg ccaacggcac ctacgtggcg gtgggtgcca ggggcgtggc
     2341 gcagaccagc acggacggca ggacgtggac gtccgtcgac accggggcga acgccgatct
     2401 cttcgccatc gcctatggct cggccggcta cgtggcggtc ggcgacttcg gcgccgtggt
     2461 ttcgagtgcc gacggcagta cctggacgag cgagcccagg ctcgcccctt cgagcgacgg
     2521 gttctggctc gagagcattg cctacggcca tggtctgtac gtcgtggccg gcgaccatgg
     2581 cgtcttcacg agcgccgacg gcgtgacctg gacgacggtg cccctgttca gccagcggat
     2641 gtcccatggt ggcgtcacga tcggcaatgg tcgcttcgtc gtggcgacaa ccgccggcac
     2701 cgcgtggagc gacgacggcg tcgcctggac actggcaccc tcggcccctc ccggcatggg
     2761 ccgcctcgtc tttggcaacg gcgtctttgt cgggagtggc accgccgggg ttgccacgag
     2821 cacggacggc gtgacctggt cgagcccggc cgtgatcggt agcaccgatg tggtcgcact
     2881 cgacttcacc cacgggcact tcctgctcga gacacccacc ctgctgtcga cctcgaccga
     2941 cggcaggacc tggaccaccg ggaacaaccc gtcggtgatc gatcccatcc agggggcaag
     3001 cgccttcacg acgacgccct cgggcgacta cctgatgctg ggcacctacg gcctggccgc
     3061 gacgagcacc gatctcgcca cctggacgcc tgccgggcag gccgaccacg gagatggctg
     3121 gttctgggtg gcctatgcca acggtaccta cctggcgatc gatgacaacg acagggcgtg
     3181 gagcagcacc gatggcggca gctgggcggc gggcacgttc tcggatccga acgacgtcaa
     3241 cttcctcacg gcggcgaacg gccggttctt cgccggcggc ggcgccgcgg gaagcctgca
     3301 gacatcgcgg gacggcgtga gctggacgac gcagtcgacc ggactcccga accgcatctt
     3361 cggcgtcacc tggggcaacg ggctctacgt cgcggtgggc gacgccggca tcgccaccag
     3421 tcccgacggt ttgacttgga cggtcgtctt gaccacgacg tctcccgaaa gctacacggc
     3481 cgtggcctac ggcgctggat tgttcgtcgc ctccgacaat cgcgatcaca tcgcgacgag
     3541 cccggacggc atccactgga cgacgggcgc gacggctttc gacagcatct tctatgggat
     3601 cgcgtacggt ccttcgggct tcgtcgcgag ttcgttgttc gaggtgtggc acagcgccga
     3661 tggcttcacg tggacgaagg acacgctgcc ggcgaacgag gtggcgccga ggaaggtgat
     3721 ctatgccgcc ggccgctacg tcggcatggg ctcgaatggt cacgtgctca ggagcacgga
     3781 cggcgtgacg tgggtggcac agccgttggg gctgccctgg aacatgtcgt cggtcgcttt
     3841 cggcggagac cgcttcgtcg ccgtcggcga acgcggcgag gtgctcgtgt ccacgcactg
     3901 agtaaccgtg tctcgaatca agcagttttg aactcgggat cccaagcaag gccgctcctg
     3961 gccatggcgt tgagcgtggt caggagcttg cgcatacaag cgatgagggc gaccttgggc
     4021 agcttgccgg cggccagcag gcgtgcgtag aaggccgcga tgggcgggtt gtggcgcgtg
     4081 gcggtcagcg tggccatgta gagcgttcgt cgtatctcga agcggccgcc gctgacgcgc
     4141 cggcggcctt tggaactgcc ggagtcgttg gccatcggcg ccacgcccac cagtgcagcg
     4201 atttgccgac ggttgagctt gccaagttcg ggcagctcgg cgatcagcgt ggcgctggcc
     4261 acaggcccga ttcctttggt cgagcgcagc aacgtgtcga gcggtccgaa atgctcctgg
     4321 acatgaccga ccatctcacc gtcgacctcg tcgagctgag ccttgatgtg ctcgatcatc
     4381 gcctcgatgc tcggacgcac cttggtcacg gccaactgca gtcgttggcg ctcggacagc
     4441 atcatcgcca gcaactgccg tcgccgagtg actaggcctg ccaagtcgcg ctgcgcctcg
     4501 gcggtgagcg gccggacgta gctggcgagg tcgggctggc gtgacagcac gtcggcgaat
     4561 gccgccagag cccgcgcgtc aatcgcgtcg gtcttggcca ggtagcccat gcccttggcg
     4621 aagtcgcgcg cctgcttcgg attgaccacg gccacggcaa agcccgcggc ctgcaacgcg
     4681 caggccaggg ccgcctcgta gccgccggtg gcctccatga ccaccagttg cacgcccaac
     4741 ggcgccagcg ctgtggccag gtcggagtga ccttcggcat cgttgacgaa acgctggggc
     4801 ttgaactcag cgcccaacac gaagacatcc acgtgcgcct tggcgacgtc gatacctacc
     4861 accgaggaaa cagacatggt cttcgactcc cgtgcttgtg catgcgcgct cgggcgaacc
     4921 cggcgctcgt aaccgttcgg gctacaggaa gaccagcacg gcagccgtgc gctctttact
     4981 cagtgacggg ctcgaacacc ctagccggga acaaactaca cgggccggtc tggttgcctc
     5041 aacggcattc agactctacc gcgctggtct gtttggaaga tacaagccg
//
LOCUS       DASVLN010000056         3171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_486512, whole genome shotgun sequence.
ACCESSION   DASVLN010000056 DASVLN010000000
VERSION     DASVLN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486512"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VF453_01215"
     CDS             <1..808
                     /locus_tag="VF453_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686287.1"
                     /translation="SPALCRPVTVVAAHCGPFTHVDPNCRSTLAGPFCHSTLPQCRPT
                     HVEPCQPTEYIHCPTGYGACPIPPYPDQAHAAANAVAAHGVTLAAPACLVTHVGCPAT
                     HVGPLCLPTAVAPLCHPTLSGPACAVTHVGCPPITLPPLCPLPTLAGPGCPATHIGCP
                     PVTVPPQCVPHTIAGPACPVTHLVCPPVTVPPHCLPQTVAGPACGHTILVQACGGVTA
                     AVVCQLTHGGPACTPATGLPGCAPSIAGCPSGIACNPGTIVNPGLPGAAG"
     gene            834..1946
                     /locus_tag="VF453_01220"
     CDS             834..1946
                     /locus_tag="VF453_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7686288.1"
                     /translation="MTIASAKIVRTLLDLGAIDAEAVVAGDLLIADNTRRHRNFRVMR
                     RSGPGCFVKQVQPGHAMADQSLHREAMCCWLAAHDARFAALRPLVPGFRAYDAARRTL
                     VLELLPDSENLSEHHDRVGRFPVEIGELLGATLGRAHAQVQVPPAQAPGLEMFPRAVP
                     WILSVHTLAAAQFERLSAANAQILAIVRQYPEFQSALDALRARWTVDGLMHGDMKWDN
                     CLVHAGPAVPGGLALRIVDWELADFGDVGWDVGAIFQSYLGAWILSMPAHEPASAEAL
                     VDRATRPIESMQPAIAAFWRRYLEVRGARGGSEGELLVRCVQYAAARMIQTAYEHMTY
                     APSLSAASLRLLQVSVNILTRPRDAIRGLLGLGDAL"
     gene            1943..2965
                     /locus_tag="VF453_01225"
     CDS             1943..2965
                     /locus_tag="VF453_01225"
                     /inference="COORDINATES: protein motif:HMM:NF037672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector HopA1 family protein"
                     /protein_id="HEX7686289.1"
                     /translation="MSAVVDTAPGTEREQAALAALAALVDATRILAPDRFVFAGRPVQ
                     LQPGAVQTLYPTPNPLVALLRDQVYEHAYVKPLHEPVPPAPAADPHFPQRLSGANAGR
                     ERWHEGWWIEAVEPQGRVVATRHALRRRFGPGEYLLRDGTFAPRPQAPIAVLQPRESH
                     GLQAGFYYAFGEALADEQDEAAQLRFYWNVRAGGVERLLANVSRTLNRFGVPFRFKCL
                     NAAEQYVRLDAAVLYVARRHDRLVRELLDELAAGLDARHLGTATPLFARRLRAGVGQA
                     EDPAGGESFGQHRCRIVAEGLWNAYVEQASTREARLACVVAQFERHGLSLRRPHLNAG
                     STLEDD"
     gene            2970..>3171
                     /locus_tag="VF453_01230"
     CDS             2970..>3171
                     /locus_tag="VF453_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686290.1"
                     /translation="MTTPLDMNDAGAFLEVADGLGARLAREAIWHGARCNWTGDAMEF
                     LDGRWQVAHRALPADVYGGTAGV"
BASE COUNT          369 a         1239 c         1125 g          438 t
ORIGIN      
        1 gagcccggcc ctgtgcaggc cggtgaccgt cgtcgcggcg cactgcgggc cgttcacgca
       61 cgtcgacccg aattgccgct cgaccctcgc cggccccttc tgtcactcga cgctgccgca
      121 gtgcaggccg acgcatgtgg agccgtgcca gcccaccgag tacatccact gcccgaccgg
      181 ctacggtgcg tgccccatcc caccctatcc ggaccaggcc catgccgcgg cgaacgccgt
      241 cgccgcgcat ggcgtgacgc tggccgcgcc tgcctgcctc gtgacgcatg tcggatgccc
      301 ggcgacgcac gtcggcccgc tgtgcctgcc taccgccgtc gcgccgttgt gtcatccgac
      361 gctgtcgggg ccggcgtgcg ccgtgaccca tgtcggttgt ccgccgatca cgcttccgcc
      421 gctttgcccg ctgccgacgc tggcgggccc cggctgcccg gccacgcaca tcggctgccc
      481 gccggtgacc gtgccgccgc agtgcgtgcc gcacacgatc gccgggcctg cgtgtcccgt
      541 gacgcacctg gtgtgcccgc ctgtcacggt gccgccgcac tgcctgccgc agacggtggc
      601 cggcccggcg tgcggccaca ccatcctcgt gcaggcctgc ggcggcgtca cggccgcggt
      661 ggtctgccag ctcacgcacg gcggcccggc gtgcacgcct gccaccggcc tgcccggctg
      721 cgcgccgtcg atcgcgggct gcccttcggg catcgcgtgc aatccgggca ccatcgtcaa
      781 cccgggcctg ccgggcgcgg cgggctgacc ggcgcgcgcg aaggagaccg cccatgacca
      841 tcgcctccgc gaagatcgtg cgcacgctgc tcgatctcgg cgcgatcgac gccgaggccg
      901 tggtcgcggg cgacctcctg atcgccgaca acacgcgtcg gcaccgcaat ttccgcgtga
      961 tgcgacgctc cggccccggc tgcttcgtca agcaggtgca gcccggccac gcgatggccg
     1021 accagtccct tcaccgcgag gcgatgtgct gctggctcgc cgcgcacgac gcgcgcttcg
     1081 ccgcgctgcg gccgctcgtg cccggcttcc gcgcctacga cgccgcgcgc cgcacgctgg
     1141 tgctggagct cctgcccgac tccgagaacc tgagcgagca ccacgaccgc gtgggccgct
     1201 tcccggtcga gatcggcgag ctgctgggcg cgacgctcgg ccgcgcgcat gcgcaggtgc
     1261 aggtaccgcc cgcgcaggcg cccggcctcg agatgttccc gcgcgcggtg ccgtggatcc
     1321 tgtcggtgca cacgctggcg gcggcgcagt tcgagcgcct gagcgccgcc aacgcgcaga
     1381 tcttggcgat cgtgcggcag taccccgaat tccagtccgc gctcgacgcc ctgcgcgcgc
     1441 gctggacggt cgacgggctc atgcacggcg acatgaagtg ggacaactgc ctcgtccacg
     1501 ccggccccgc agtgccgggc ggcctcgcgc tgcgcatcgt cgactgggag ctcgcggact
     1561 tcggtgacgt cggctgggac gtcggggcga tcttccagtc ctacctgggc gcgtggatcc
     1621 tctccatgcc ggcccacgag cccgcgagcg ccgaggcgct ggtcgaccgg gcgacgcggc
     1681 ccatcgagtc catgcagcct gccatcgccg ccttctggcg ccgctacctc gaggtgcgcg
     1741 gtgcgcgcgg cggcagcgag ggcgagctgc tcgtgcgctg cgtgcagtac gccgcggcgc
     1801 gcatgatcca gaccgcctac gagcacatga cctatgcgcc cagcctgtcg gccgcgagcc
     1861 tgcgcctgct gcaggtcagc gtgaacatcc tcacgcgccc gcgcgacgcg atccgcggcc
     1921 tgctggggct gggagacgcg ctgtgagcgc ggtcgtcgat acggcaccgg gtacggagcg
     1981 cgagcaggcc gcgctcgccg ccctcgcggc cctggtcgac gcgacgcgga tcctcgcgcc
     2041 cgaccgcttc gtcttcgcgg gccggccggt gcagctgcag cccggcgccg tgcagacgct
     2101 ctacccgacg cccaaccccc tcgtggcgct gctgcgcgac caggtctacg agcatgccta
     2161 cgtcaagccg ctgcacgagc cggtgccgcc cgcgccggcg gccgatccgc atttcccgca
     2221 gcggctgtcg ggcgccaacg ccggccgcga gcgctggcac gagggctggt ggatcgaggc
     2281 ggtcgagccg cagggccggg tcgtcgcgac ccggcacgcg ctgcgccgcc gtttcggccc
     2341 cggcgaatac ttgctgcgcg acggcacctt cgcgccgcgc ccgcaggcgc cgatcgccgt
     2401 gctgcagccg cgcgagtcgc acggcctgca ggcgggcttc tactacgcct tcggcgaggc
     2461 gctggccgac gagcaggacg aggccgcgca gctgcgcttc tactggaacg tccgcgcggg
     2521 cggcgtcgag cggctgctcg ccaacgtctc gcgcaccctc aaccgcttcg gcgtgccgtt
     2581 ccgcttcaag tgcctcaacg cggcggagca gtacgtgcgg ctcgatgcgg cggtgctcta
     2641 cgtcgcgcgc cgccacgacc ggctcgtgcg cgagctgctc gacgagctcg ccgccggcct
     2701 cgacgcgcgg cacctgggca cggcgacgcc actgttcgcg cgccgcctgc gcgcgggcgt
     2761 ggggcaggcc gaggatcccg ccggcggcga gagcttcggc cagcaccgct gccgcatcgt
     2821 cgccgagggg ctgtggaacg cctacgtgga gcaggcgtcg acgcgcgagg cgcggctcgc
     2881 ctgcgtcgtc gcgcagttcg agcgccatgg cctgtcgctg cggcgcccgc acctgaatgc
     2941 cggctcgacg ctggaggacg attgacgcca tgaccacgcc cctcgacatg aacgacgccg
     3001 gcgccttcct cgaggtcgcc gacggcctgg gcgcgcgcct cgcgcgcgag gcgatctggc
     3061 acggcgcgcg ctgcaactgg acgggcgacg cgatggagtt cctcgacggc cgctggcagg
     3121 tcgcgcatcg cgcgctgccc gccgacgtct acggcggcac ggcgggcgtc g
//
LOCUS       DASVLN010000057         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_56591, whole genome shotgun sequence.
ACCESSION   DASVLN010000057 DASVLN010000000
VERSION     DASVLN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56591"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..698)
                     /locus_tag="VF453_01235"
     CDS             complement(84..698)
                     /locus_tag="VF453_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686291.1"
                     /translation="MQALQRQRTLAALAIAFAAVATTAAHAAPAPAPAASAAAPAPID
                     PDKRKAVDRVIADWHPEGAMIAGAQGAVARAFDQSILILQEKHVAPEKIEQARKEMLP
                     EAQKSADKVGALAKASALKNVPTVVAPALAQNFSNDELAKIAAVMEAMEQPAWKKFQQ
                     FVPQVNQAMGRKVDAEAGADINKELKAVSETLGNKVRATVGLGN"
     gene            complement(714..2387)
                     /locus_tag="VF453_01240"
     CDS             complement(714..2387)
                     /locus_tag="VF453_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX7686292.1"
                     /translation="MQATTRPARRPARSAARADHPLRPAAAAALAFAGVLAAPGAHAA
                     LTENLAVSPVAMSMGNAVTADPPGLDSIHFNPAGLSKVEGDTRSDTLFGASLRVTAKF
                     HQPGGFDIGGWTQDPLNGTTSDHNKQAIFIPIVGKASPRLPIAVAAGLALSWHNEGSP
                     WTFATGTYVPQAVGIDRTKNPDDPARFDGKQVVIQRLVYLSPSVSYKVNDEFSLGVAI
                     PIAHQGFALDTDMRFPNKLLGIIGKLQNAWCGDSGNPLDTLGFGLCGTDSTGKQSGRL
                     NPFNEVGEMKFAATAPADITYNIGALWNPTDYLGFGAVYQSGSKTTMTGTYNFRADPM
                     LPEFVRHMYSSLFGPIVAATFGFPTSIPTNQGGNFTLVLPYPAHFQFGVKLKPVSRVQ
                     LDLDAGWTDWSKWNQLTFQFDQQVKLLEMARLFGQADSSKLVIPRGYHSVWSYGAGLQ
                     LDVWDGVKLRLGYEPRKSSVPNNKIDLIAPMPDLPVKSIGLSYEMKNGMKLETGFSYA
                     RGHYYVPAGTDCNLNCDNFFNIIYNPYAGLDVSGTLVLRYFGMKLTQPF"
     gene            complement(2400..>3323)
                     /locus_tag="VF453_01245"
     CDS             complement(2400..>3323)
                     /locus_tag="VF453_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7686293.1"
                     /translation="KKQAGDVDQSTLLKDTLTKSERQYSLLKAGRIGMNYDLNYSYEG
                     SEAINEGFDTTGAIDLFKIETTRAHTITNSVSLDYGLLDNLTLNTTIPLVSKFTQSPE
                     FGGVANALGDITLGGRYQPFVQTAGGPTFTVTGQLSIPTGRSPFDMVMGHNLATGSGY
                     ASGTLGLNASQVYDPVALFGSVNFTEAASANGLHQYNNVDQSTLLKVKPGKSVGIGAG
                     FTYALSYNISTSVSFQETVTTASTITYRDASNNVLSRSTAQQVAAILNFGLGVRLNPK
                     TTVNLSAGVGLTTDSPDFSLGLDVPLTFSFF"
BASE COUNT          466 a         1037 c         1231 g          589 t
ORIGIN      
        1 cgtcgctgcc gccgccgcag gcgacgaggg cgaacagcgc gccgagcgcc gccagcgtgc
       61 ggggcgtggc catcggggag gcgtcagttg ccgaggccga ccgtggcgcg gaccttgttg
      121 ccgagcgtct cgctgaccgc cttcagctcc ttgttgatgt cagcacccgc ctcggcgtcg
      181 accttgcggc ccatcgcctg gttgacctgc gggacgaact gctggaattt cttccacgcc
      241 ggctgctcca tcgcctccat gacggcggcg atcttggcga gctcgtcgtt gctgaagttc
      301 tgcgccagcg ccggcgcgac cacggtcggc acgttcttca gcgcggaggc cttggcgagc
      361 gcgcccacct tgtcggcgga cttctgcgcc tcgggcagca tctccttgcg cgcctgctcg
      421 atcttttccg gcgcgacgtg cttctcctgc aggatcagga tggactggtc gaacgcgcgc
      481 gcgacggcgc cctgcgcgcc ggcgatcatg gcgccctcgg gatgccagtc ggcgatcacg
      541 cggtcgacgg ccttgcgctt gtcgggatcg atgggcgccg gggcggcggc ggacgcggcg
      601 ggagccggcg cgggcgcggc gtgggcggcg gtcgtggcga cggcggcgaa ggcgatggcc
      661 agggcggcca gggtgcgttg gcgttggagg gcttgcatgg gtcgtcagga gggtcagaag
      721 ggttgcgtga gcttcatgcc gaagtagcgc agcacgagcg tgcccgagac gtcgaggccg
      781 gcgtacgggt tgtagatgat gttgaagaag ttgtcgcagt tgaggttgca gtccgtgccc
      841 gcagggacgt agtagtgccc gcgcgcatag ctgaagccgg tctccagctt catgccgttc
      901 ttcatctcgt agctcaggcc gatgctcttg acgggcaggt cgggcatcgg cgcgatgagg
      961 tcgatcttgt tgttcggcac ggagctcttg cgcggctcgt agcccaggcg cagcttgacg
     1021 ccgtcccaga cgtccaactg tagcccggcg ccgtagctcc agacgctgtg gtagccgcgc
     1081 gggatgacca gcttgctcga atcggcctgg ccgaacaggc gcgccatctc gagcagcttg
     1141 acctgctggt cgaactggaa ggtgagctgg ttccacttgg accagtcggt ccagcccgcg
     1201 tccaggtcga gctgcacgcg gctgaccggc ttcagcttga cgccgaactg gaagtgcgcc
     1261 gggtagggca gcacgagcgt gaagttgccg ccctggttgg tcgggatgct ggtcgggaag
     1321 ccgaaggtcg ccgcgacgat cggcccgaac agcgacgagt acatgtggcg cacgaactcc
     1381 ggcagcatgg gatccgcgcg gaagttgtac gtcccggtca tcgtcgtctt ggagccggac
     1441 tggtagaccg cgccgaagcc gaggtagtcg gtcgggttcc acagcgcgcc gatgttgtag
     1501 gtgatgtcgg ccggcgcggt cgccgcgaac ttcatctcgc cgacctcgtt gaacgggttc
     1561 aggcggccgc tctgcttgcc ggtggagtcc gtgccgcaca ggccgaagcc cagcgtgtcc
     1621 agcgggttgc cgctgtcgcc gcaccaggcg ttctgcagct tgccgatgat gcccagcagc
     1681 ttgttcggga agcgcatgtc ggtgtcgagc gcgaagccct ggtgggcgat cgggatcgcg
     1741 acgccgaggc tgaactcgtc gttgaccttg tagctcaccg acggcgacag gtagaccagg
     1801 cgctggatca cgacctgctt gccgtcgaac cgtgccggat cgtcgggatt cttggtgcgg
     1861 tcgatgccca cggcctgcgg gacgtaggtg cccgtcgcga aggtccaggg cgagccctcg
     1921 ttgtgccagg acagcgcgag gccggcggcc accgcgatcg gcaggcgcgg gctcgccttg
     1981 ccgacgatgg ggatgaagat cgcctgcttg ttgtggtcgg aggtggtgcc gttgagggga
     2041 tcctgcgtcc agccgccgat gtcgaagccg ccgggctggt ggaacttggc cgtcacgcgc
     2101 agggacgcgc cgaacagcgt gtcggagcgc gtgtcgccct cgaccttcga caagcccgcc
     2161 gggttgaagt ggatcgagtc gaggccgggc gggtcggcgg tcaccgcgtt gcccatcgac
     2221 atcgccaccg ggctcacggc caggttctcg gtcagcgccg cgtgcgcgcc gggcgcggcg
     2281 aggacgcccg cgaaagccag cgccgcggcg gccgccgggc gcagcggatg gtccgcgcgg
     2341 gcagcggatc gcgccgggcg gcgcgcgggg cgcgtcgtcg cttgcatcgt ggtcggccct
     2401 cagaagaacg agaaggtcag cgggacgtcg agcccgaggc tgaagtccgg cgagtcggtc
     2461 gtcaggccca cgccggcgct caggttgacc gtcgtcttcg ggttcaggcg cacgcccagg
     2521 ccgaagttca ggatcgcggc cacctgctgc gccgtcgacc ggctgagcac gttgttggag
     2581 gcgtcgcgat acgtgatggt cgaggccgta gtgacggtct cctggaacga cacgctggta
     2641 gagatgttgt atgacagcgc gtaggtaaaa ccggcgccga taccgacgct cttgcctggc
     2701 ttgaccttca gcagtgtgga ctggtcgacg ttgttgtact ggtgcagccc gttggccgac
     2761 gcggcctcgg tgaagttgac cgagccgaac agcgccacgg gatcgtagac ctgcgaggcg
     2821 ttcagcccca gggtgcccga cgcgtagccc gagccggtgg ccaggttgtg gcccatcacc
     2881 atgtcgaacg ggcttcggcc cgtggggatg ctcagctggc ccgtgacggt gaaggtcggc
     2941 ccgccggccg tctgcacgaa gggctggtag cgcccgccca gcgtgatgtc gccgagcgcg
     3001 ttggcgacgc cgccgaactc ggggctctgc gtgaacttgc tgaccagcgg gatcgtcgtg
     3061 ttcagggtca ggttgtccag caggccgtag tcgagcgaca ccgagttggt gatcgtgtgg
     3121 gcgcgcgtcg tctcgatctt gaacaggtcg atcgcgcccg tggtgtcgaa gccctcgttg
     3181 atcgcctcgc tgccctcgta gctgtagttg aggtcgtagt tcatgccgat gcgccccgcc
     3241 ttcagcagcg agtactggcg ctcggacttg gtcagcgtgt ccttcagcag cgtggactgg
     3301 tcgacgtcgc cggcctgctt ctt
//
LOCUS       DASVLN010000058         7830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_164602, whole genome shotgun sequence.
ACCESSION   DASVLN010000058 DASVLN010000000
VERSION     DASVLN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164602"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1960
                     /locus_tag="VF453_01250"
     CDS             <1..1960
                     /locus_tag="VF453_01250"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX7686294.1"
                     /translation="SSAGAAQAAGPATSDSATASAAADASGEVDAPATAADRAPLAGP
                     PEPVLLGHLVLLHDLSFIERRSQDTRQYLVGFIAVLGLIIALITVVVAQLSWRGWVHG
                     VQALLRGEGLVNPIMPAPELGPFVADLRERLRELEDEHRRMQGPQAEWTAERLRALLR
                     TQLRGDQVIVVSNREPYIHEKADGGIEVRRPASGLVTAVEPVMRACSGTWIAHGSGSA
                     DREVVDRHDRVSVPPGCDDYRLRRIWLTQAEEDGYYYGFANEGLWPLCHVAHVRPVFR
                     ESDWAAYREVNRRFADAVVAEARGEDPIVLVQDYHFALLPAMIRERLPQATILTFWHI
                     PWPNPESFGICPWRREILQGMLGSTILGFHTQFHCKNFIETVDRFLEARIEHEHSTIT
                     YQGKETLVEDYPISIEWPGPAAVAHREPVEACRQRVIGRLGLPAGVKLAVGIDRFDYT
                     KGILERLNAVERLLEKFPEWLGRFVFVQVAAPTRSALYEYRAFQARIEEVVARINERF
                     GSPGYLPVHLLARHHEPAAVEELFRAADVCVVTSLHDGMNLVCKEFVAARDDEQGVLI
                     LSRFAGAAREMTEALIVNPYHVEETADALDRAIRMPAAEQRERMASLRSTVREFNVFR
                     WAGRMLADAARWRLRSRIDARVRRYGEG"
     gene            1957..2763
                     /gene="otsB"
                     /locus_tag="VF453_01255"
     CDS             1957..2763
                     /gene="otsB"
                     /locus_tag="VF453_01255"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX7686295.1"
                     /translation="MTDVPPLLLAPEGLAELARIARAATLYAFDFDGTLAPIGPSRQN
                     VRIERAVSTLLHELARRAPVAIVTGRRVEDVRRRLGFVPVAIVGNHGAEDPARPRATT
                     RWTRALSSLRQCLRGHAAALEAARIAVEDKGPSIALHYRAAPDPAAALALVDRVLEPA
                     GADLRRFEGKCVVNLVAADAPDKADAVSRLLDARGCGTAFYAGDDVNDEPVFASARPD
                     WVTAKVGLERGTRARFLVAEQRDIREVVLRLLDAVGSGKPPPRQSSASGV"
     gene            complement(2739..3245)
                     /locus_tag="VF453_01260"
     CDS             complement(2739..3245)
                     /locus_tag="VF453_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7686296.1"
                     /translation="MTQSKQAGKPALATPPGVDSRLVLRQFRQIFNAVKAHFRQVENK
                     AGLGGAQVWALGLIRDQPGIGVSELARSMDVHPSTASNLVRSLTEKGLVAAGKDDVDR
                     RSVQLCVLPAGVRALRMAPGPREGVLPEALESLDPALLARLHADLGVLIQTLQAEGHG
                     AQTPLAEL"
     gene            complement(3469..4839)
                     /locus_tag="VF453_01265"
     CDS             complement(3469..4839)
                     /locus_tag="VF453_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX7686297.1"
                     /translation="MTLAGPTPPPEPASAAPGVQALISFLEHDPQPAIVLGPDYGILA
                     ANTAYRRQFGTADEPHVGRKCFRVSHGYDVPCDQAGEHCPMRRASETQGPDRVLHVHH
                     TPRGPEHVDVELRPILDERHRVVAYVERLVTVRQASARPSSEGLVGRSAAFGAALSAV
                     QRVAKSTLAVLLLGESGSGKELFARAVHEASARAAGPFVIVDCSGFVEALFESELFGY
                     ERGAFTGATARKQGLVETASGGTLFLDEIGDVPLPMQVKLLRLIETGTYRRVGGVETL
                     QADFRLVAATHKPLPAMVADGRFRQDLYYRISAFPIELPPLRARRDDIPVLVESILQR
                     APGGTAGIVVDAEAMAMLKRHDWPGNVRELRNVLDRARLFADDGVIRSEHLPETLHRR
                     PEVPTARVQGPAPPRVDDDALRRLVAERRLTRKEIAEQLGLSERTLYRRLKDCGLARD
                     PRPGSS"
     gene            5181..5426
                     /locus_tag="VF453_01270"
     CDS             5181..5426
                     /locus_tag="VF453_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686298.1"
                     /translation="MDNRRQTVDSPPPARGRRRKHPPGRLGHHLAVTVAAKVAILAAL
                     WFAFFHHPAQGPARPRSAADATAAHVLGTDAPQGVSP"
     gene            5423..7003
                     /locus_tag="VF453_01275"
     CDS             5423..7003
                     /locus_tag="VF453_01275"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX7686299.1"
                     /translation="MISEQLVDLSRLQFAVTALYHFLFVPLTLGMVWLLVIMETVYVT
                     TGRVIWKDMTRFWGKLFGINFALGVTTGITMEFQFGTNWAYYSHYVGDVFGAPLAIEG
                     LMAFFLESTFIGLFFFGWDRLSRKQHLLVTILMAVGTNLSALWILIANGWMQNPVGAE
                     FNFRTMRMEVTDFWAVVFNPDAQAKFVHTVSAGYVTGSMFVLSISAWYLLRGRNLEFA
                     RRSFRVAAAFGLASAASVIVLGDESGYTVGEAQQTKLAAIEAMWKTEPAPAPFTLVAW
                     PDDAAQRNDWEVKIPYVMGLIGTRSLTRVIPGIDEIKAKNRTRIEHGLVALKALEALR
                     KDREDARARAAFEANKADLGFGLLVKRYVADASSATPEVIQRAVDDSMPRVAPMFWSF
                     RIMVGLGFAMLALFAVAFWNTLRMRCVGKPWLLKWALWMLPAPWISAELGWYVAEYGR
                     QPWTIYGVLPTHLSVSSLSAGDLYGSMAGFVGFYTLLLVVEVYLMQRFARLGPGSLGT
                     GRYDNEPPTHAPAGAAAA"
     gene            7016..>7830
                     /gene="cydB"
                     /locus_tag="VF453_01280"
     CDS             7016..>7830
                     /gene="cydB"
                     /locus_tag="VF453_01280"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013693155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX7686300.1"
                     /translation="MFDYAVLKLLWWGLVGVLLVGFAIMDGHDMGVGTLLPFVGRGDD
                     ERRAVINTIGPHWDGNQVWFITAGGAVFAAWPLVYATAFSGFYWALLAVLWALFFRPV
                     GFDYRSKVSDPRWRSAWDWSLFVGGAVPPLIFGVAFGNLLQGVPFTFDAMMVSTYTGS
                     FWALLNPFALLAGVVSSAMITLQGATYLAHRTEGDIQDRAVRAARSAAVAMVVAFVAA
                     GLWLKLGRIEGYAIASAVDPSAPADPLAKTVVRSADAWWANYGRAPALWLAPAL"
BASE COUNT         1076 a         2776 c         2785 g         1193 t
ORIGIN      
        1 gtcgtccgct ggcgccgcgc aggccgccgg gccggctacc tcggacagcg ccacggcgag
       61 cgcggcggcg gacgcctcgg gcgaggtcga tgccccggcg acggcggccg atcgtgcccc
      121 cctcgcgggg cctccggagc cagtgctgct cggccacctg gtcctgctgc acgacttgag
      181 cttcatcgag cgccgcagcc aggacacgcg ccaatacctg gtgggattca tcgccgtgct
      241 gggcctgatc atcgcgttga tcacggtggt ggtggcccag ctgagctggc gcggctgggt
      301 gcacggcgtg caggcgctgc tgcgcggcga gggcctggtg aacccgatca tgccggcgcc
      361 cgaactcggg cctttcgtcg ccgacctgcg cgagcgcctg cgcgaactcg aggacgagca
      421 ccgccgcatg cagggtccgc aggccgagtg gaccgccgag cgcctgcgcg cgctgttgcg
      481 cacccagctg cgcggcgacc aggtcatcgt cgtctcgaat cgcgagccct acatccacga
      541 gaaggcggac ggcggcatcg aagtccggcg tccggccagt gggctcgtca cggcggtgga
      601 gcccgtgatg cgcgcgtgct ccgggacctg gatcgcccac ggcagcggca gcgccgaccg
      661 cgaggtcgtc gaccgccacg accgcgtcag cgtcccgccg ggctgcgacg actaccggct
      721 gcgacggatc tggctgacgc aggccgagga ggacggctac tactacggct tcgccaacga
      781 gggcctgtgg ccgctgtgcc acgtcgcgca cgtgcgcccc gtcttccgcg aatccgactg
      841 ggccgcgtac cgggaggtca accgccggtt cgccgacgcg gtggtggcgg aggcgcgcgg
      901 cgaggatccc atcgtcctcg tgcaggacta ccacttcgcg ctgctgccgg cgatgatccg
      961 cgagcgcctg ccccaggcca ccatcctgac gttctggcac atcccctggc cgaatcccga
     1021 gtccttcggc atctgcccgt ggcgacgcga gatcctgcag ggcatgctgg gcagcaccat
     1081 cctcggcttc cacacccagt tccactgcaa gaacttcatc gagaccgtcg atcgcttcct
     1141 tgaggcgcgg atcgagcacg agcactcgac gatcacctac cagggcaagg agacgctcgt
     1201 cgaggactac ccgatctcca tcgagtggcc cggcccggcc gccgtcgcgc accgcgaacc
     1261 ggtggaggcc tgccgccagc gggtaatcgg ccgcctcggg ctgcccgcgg gcgtgaagct
     1321 ggcggtcggc atcgaccgct tcgactacac gaagggcatc ctcgagcggc tcaacgcggt
     1381 cgagcgcctc ctcgagaaat tccccgaatg gctggggcgc ttcgtcttcg tgcaggtcgc
     1441 cgcgccgacg cgcagcgcgc tctacgaata ccgggccttc caggcgcgca tcgaggaggt
     1501 ggtggcgcgc atcaacgagc gcttcggctc gccgggctac ctgccggttc acctgctggc
     1561 gcgccaccac gagccggcgg ccgtcgagga gctcttccgc gccgcggacg tctgcgtcgt
     1621 caccagcctg cacgacggga tgaacctcgt ctgcaaggag ttcgtcgccg cgcgcgacga
     1681 cgagcagggc gtgctgatcc tgagccgctt cgcgggcgcc gcgcgcgaga tgacggaggc
     1741 gctgatcgtc aatccgtacc acgtcgagga gaccgcggac gcgctcgacc gggcgatccg
     1801 catgccggcg gccgaacagc gcgaacgcat ggccagcctg cgcagcacgg tgcgcgagtt
     1861 caacgtcttc cgctgggccg ggcgcatgct ggccgacgcg gcgcgctggc ggctgcgctc
     1921 gcgcatcgac gcgcgcgtgc ggcgctacgg cgaaggatga cggacgtccc gccgctgctg
     1981 ctggcgccgg aggggctggc cgagctcgcc cgcatcgcgc gtgcggcgac actctacgcc
     2041 ttcgacttcg acggcacgct cgccccgatc ggccctagcc ggcagaacgt ccgcatcgag
     2101 cgggcggtct ccacgttgct gcacgagctg gcacggcggg cgccggtggc gatcgtcacc
     2161 ggtcgccggg tcgaggatgt gcgccgacgg ctgggcttcg tgcccgtggc gatcgtcggc
     2221 aaccatgggg cggaggatcc ggcgcggccg cgcgcgacga cgcgctggac gcgcgcgctg
     2281 tcgtcgctgc gccagtgcct gcgcggccac gccgccgcgc tggaggccgc gcgcatcgcc
     2341 gtcgaggaca aggggccgtc gatcgcgctg cactaccgcg cggcgccgga tccggcggcg
     2401 gccctggcgc tcgtcgatcg cgtgctggag ccggcgggtg cggacctgcg tcgattcgaa
     2461 ggcaaatgcg tcgtcaacct cgtcgccgcg gacgcccccg acaaggcgga cgcggtcagc
     2521 cggctgctcg acgcccgcgg atgcggcacc gcgttctacg ccggcgacga cgtcaacgac
     2581 gagcccgtgt tcgcgtcggc gcgcccggac tgggtcaccg ccaaggtcgg cctcgagcgc
     2641 gggacccggg cccgcttcct cgtcgccgag cagcgcgaca tccgcgaggt cgtccttcgc
     2701 ctgctcgacg ccgtggggtc cggcaagccg ccgccgcgtc aaagctccgc cagcggcgtc
     2761 tgagcgccgt ggccttcggc ctgcagcgtc tggatcagca cgccgaggtc ggcatgcagg
     2821 cgcgcgagca atgcggggtc gagcgattcg agcgcttcgg gcagcacgcc ctcgcgcggg
     2881 cccggcgcca tccgcagcgc gcgcacgccc gccggcagga cgcacagctg caccgagcgg
     2941 cggtcgacgt cgtcctttcc cgcggcgacc agtcccttct cggtcagcga ccgcacgagg
     3001 ttgctggcgg tcgacggatg cacgtccatg gagcgcgcga gctcgctgac gccgatgccg
     3061 ggctggtcgc ggatcaggcc gagcgcccag acctgggcgc cgcccagtcc cgccttgttt
     3121 tccacctgcc ggaagtgcgc cttgacggca ttgaagatct gccggaactg gcgcagcacc
     3181 agcctggagt cgacgcccgg cggggtcgcg agagcgggtt tgccggcctg cttcgattgc
     3241 gtcatggccc ggatgatacg aaagggcgtc agcgcccatg cgcgagcgtg cctggcggcc
     3301 tcgaccaaaa agaacgggca cccgggggtg ccctgaaaca tgcgaggctc tcaagtcgcc
     3361 gacctgccga tgcccgcggc tcgagtcgcg gaagagggca agtcgcctgg gacggattat
     3421 atatgtttgt gcaaacgtat catccagcgc ttcgagcctc gaaggcgctc agctggatcc
     3481 gggacgcgga tcgcgggcca ggccgcagtc cttgaggcgc cggtaaagcg tccgctcgct
     3541 caggcccagc tgctcggcga tctcctttcg cgtcagcctg cgctccgcca cgaggcggcg
     3601 gagggcgtcg tcgtcgacgc gcggcggcgc agggccctgc acgcgcgcgg tgggcacctc
     3661 ggggcggcga tgcagcgttt ccggcaggtg ctcgctgcgg atgaccccat cgtcggcgaa
     3721 caggcgcgcg cgatccagca cgttgcgcaa ctcccggacg ttgcccggcc agtcgtgccg
     3781 cttcagcatg gccatggcct cggcatcgac gacgatgccc gccgtcccgc cgggggctcg
     3841 ctgcaggatc gactcgacga gcacgggaat gtcgtcgcgc cgcgcgcgca gcggtggcag
     3901 ctcgatcggg aaggcgctga tccggtagta caggtcctgg cggaagcggc catccgcgac
     3961 catggccggc agcggcttgt gcgtggccgc caccaggcga aagtccgcct gcagcgtctc
     4021 gacgccgccg acccggcggt aggtgccggt ctcgatgagc cgcagcagct tcacctgcat
     4081 cggcagcggg acgtcgccga tctcgtcgag gaacagcgtg ccgccgctcg cggtttcgac
     4141 caggccctgc ttgcgcgccg tggcccccgt gaaggcgccg cgctcgtagc cgaacagctc
     4201 gctctcgaac agcgcctcga cgaagcccga gcagtccacg atgacgaagg gcccggccgc
     4261 ccgcgcgctc gcctcgtgga cggcgcgcgc gaacagctcc ttgcccgaac cggactcgcc
     4321 caggagcaac accgccagcg tcgacttcgc cacgcgctgc accgccgaca gcgcggcgcc
     4381 gaaggcggcg gatcgcccga ccaggccttc ggagctcggc ctggccgacg cctggcgcac
     4441 ggtcacgagc cgttcgacat aggccacgac ccggtgccgc tcgtcgagga tggggcgcag
     4501 ctccacgtcc acgtgctccg ggccgcgcgg cgtgtggtgg acgtgcagca cgcggtcggg
     4561 gccctgggtc tcggacgcgc gcctcatcgg gcagtgttcg ccggcctggt cgcacgggac
     4621 gtcgtagccg tgcgagacgc ggaagcactt gcggccgaca tgcggctcgt cggccgtgcc
     4681 gaactgccgc ctgtacgccg tgttcgcggc gaggatgccg tagtcggggc cgagcacgat
     4741 cgcgggctgc ggatcgtgct cgaggaagga gatcagcgcc tgcaccccgg gtgcggcgct
     4801 cgcgggttcg ggcggtggcg tcgggcctgc cagggtcatg ccgccattgt ctgccaggct
     4861 gccaatttct gccggtatgg caggggcgcc gtcgcagccg ccccggcggt tgcggcgcgc
     4921 gaagcgcgga ttccttctgc atcaacgggt tacgccggga tcgctgcgac cggtcgactt
     4981 ggcacggatg ttgtttcaag aggatggaca gcgccggatg cgctgccaat ccgaatcgac
     5041 agggaggccc ggtggccggg ggttgcgctt catcagcccc gtgcgcgaag agcacgggca
     5101 taacgtgcat gtgcgcgacg gcatcagcga ggacaggctc ctggcgaacc ggcccgatcg
     5161 cgcttcacgg gacacatgac atggacaacc gccgccagac ggtcgactcg ccgccgccgg
     5221 cccgcgggcg ccgcaggaaa catccgcccg gacgcctcgg acaccacctc gccgtgaccg
     5281 tcgccgcgaa ggtcgcgatc ctggcggcgc tgtggttcgc cttcttccac cacccggccc
     5341 agggcccggc ccgcccgcga tccgcggccg acgccacggc cgcccacgtc ctcgggacgg
     5401 acgccccgca aggagtctcg ccttgatctc ggaacaactc gtcgacctgt cgcgcctgca
     5461 attcgccgtc accgcgctct accacttcct gttcgtgccg ctcacgctgg gcatggtgtg
     5521 gctgctggtc atcatggaga cggtctacgt caccaccggc cgcgtgatct ggaaggacat
     5581 gacgcgcttc tggggcaagc tgttcggcat caatttcgcg ctcggcgtca ccacgggcat
     5641 cacgatggag ttccagttcg gcacgaactg ggcctattac tcgcactacg tcggcgacgt
     5701 gttcggcgcg cccctggcga tcgaaggcct gatggcgttc ttcctggagt cgaccttcat
     5761 cggcctgttc ttcttcggct gggaccgcct gagccgcaag cagcacctgc tcgtgaccat
     5821 cctgatggcc gtgggcacca atctgtcggc gctgtggatc ctgatcgcga acggctggat
     5881 gcagaacccg gtgggcgccg agttcaactt ccggacgatg cgcatggagg tcacggactt
     5941 ctgggcggtg gtgttcaatc ccgacgccca ggccaagttc gtgcacaccg tctcggcggg
     6001 ctacgtcacc ggctcgatgt tcgtgctgtc catctccgcc tggtacctgc tgcgcgggcg
     6061 caacctggag ttcgcgcggc gcagcttccg cgtcgcggcg gcgttcggcc tggcctccgc
     6121 ggccagcgtc atcgtgctgg gcgacgaatc gggctacacg gtgggcgagg cgcaacagac
     6181 caagctggcg gccatcgagg cgatgtggaa gacggagccg gcgccggcgc ccttcacgct
     6241 cgtcgcgtgg cccgacgacg ccgcgcagcg caacgactgg gaagtcaaga ttccctacgt
     6301 gatgggcctg atcggtacgc gcagcctcac gcgcgtcatc ccgggcatcg acgagatcaa
     6361 ggcgaagaac cggacgcgca tcgagcatgg cctcgtggcg ctgaaggcgc tggaggcctt
     6421 gcgcaaggat cgcgaggatg cgcgcgcccg cgcggcgttc gaggcgaaca aggccgacct
     6481 cggcttcggc ctgctggtca agcgctacgt cgccgacgcc tccagcgcca cgcccgaggt
     6541 catccagcgg gcggtggatg acagcatgcc gcgcgtggcg ccgatgttct ggagcttccg
     6601 catcatggtc ggcctgggct tcgcgatgct ggcgcttttc gcggtcgcgt tctggaacac
     6661 gctgcgcatg cgatgcgtcg gcaagccgtg gctcctgaag tgggcgctgt ggatgctgcc
     6721 ggcgccatgg atctcggccg agctcggctg gtacgtggcc gagtacggcc gccagccgtg
     6781 gacgatctac ggcgtcctgc ccacgcacct gagcgtgtcc agcctgtccg cgggcgacct
     6841 gtacggctcg atggccggct tcgtcgggtt ctacacgctg ctgctggtgg tggaggtcta
     6901 cctgatgcag cgcttcgcgc gactgggtcc cggcagcctg ggcaccggac gctacgacaa
     6961 cgaaccgccc acgcatgcac cggcgggtgc ggcggcggcc tgaaggagtc cacgcatgtt
     7021 cgactacgca gtcctcaagc tcctctggtg gggcctcgtc ggcgtcctgc tcgtcggctt
     7081 cgccatcatg gacggccacg acatgggcgt gggcacgctg ctgcccttcg tgggccgcgg
     7141 cgacgacgag cggcgcgccg tgatcaacac gatcggcccg cactgggacg gcaaccaggt
     7201 gtggttcatc accgccggcg gcgccgtctt cgccgcctgg ccgctggtgt acgccaccgc
     7261 gttcagcggc ttctactggg cgttgctggc ggtgctgtgg gcgctgttct tccgcccggt
     7321 cggcttcgac taccgcagca aggtctccga cccgcgctgg cgctccgcct gggactggag
     7381 cctgttcgtg gggggggcgg tgccgccgct gatcttcggc gtcgccttcg gcaacctgct
     7441 gcagggcgtg ccgttcacct tcgacgcgat gatggtctcg acgtacacgg gctcgttctg
     7501 ggcgctgctc aacccgttcg cgctgctggc gggcgtggtc tcgagcgcca tgatcacctt
     7561 gcagggtgcg acctacctgg cgcaccgcac cgagggcgac atccaggatc gcgccgtgcg
     7621 tgccgcccgg tcggccgcgg tggcgatggt ggtggccttc gtcgccgccg ggctgtggct
     7681 gaagctcggc cggatcgagg ggtacgccat cgcctcggcg gtcgatccgt cggcgcccgc
     7741 cgacccgctg gccaagaccg tcgtgcgcag cgccgacgcc tggtgggcca actacggccg
     7801 ggcgcccgcg ctctggctcg cgccggcgct
//
LOCUS       DASVLN010000059         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_325546, whole genome shotgun sequence.
ACCESSION   DASVLN010000059 DASVLN010000000
VERSION     DASVLN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325546"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VF453_01285"
     CDS             complement(<1..612)
                     /locus_tag="VF453_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7686301.1"
                     /translation="MTRAAAPAASAHPAHGWHLRKKRLLMALLGALVGLEFMENAMFV
                     FAASHVVGGIDAAPREFAQVQAAYAVGSMLMIVLQQTFARHLGYRRYLTGALALFMAG
                     AAACAGSGGLASLTLARLVQGFGGGALFTSSRVLVPLLFPAAERPRALKWFMLLLFGM
                     SAAGPLLAAWLVEDASWRWVFLPALPVGAVVLAGAWTLLPDGLG"
     gene            complement(609..1097)
                     /locus_tag="VF453_01290"
     CDS             complement(609..1097)
                     /locus_tag="VF453_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013951861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7686302.1"
                     /translation="MSFESRLDRLLAGKPEELADAVRASRLVFRVAHLLQSRIDAALA
                     PLGIDMREYLALVLVGDDADALRPSDLSATLDATRTQVTRLLDGLEAKGLVRRAPNSA
                     DRRSLQLESTAAGKALTRRAAQAVHPAYAAAWSGLGPESTRRTLRALTRVHATLSGPD
                     AP"
     gene            complement(1241..1747)
                     /locus_tag="VF453_01295"
     CDS             complement(1241..1747)
                     /locus_tag="VF453_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HEX7686303.1"
                     /translation="MKLVLAHRLDGDTVTAAGVAFEDWGDVEAARTVMARVPAGPKPA
                     RGEADLRALPALLQLLQDHRLQPDAIVMDDPVHLDPQDTPGLGRRLFDALDGKVPVIG
                     ASKGAMAGGTPAQFEVHREDEAAPLTVTCAGIDLGAAKARVRAMHGRKRVPTLLKLAA
                     RLAKGGGD"
BASE COUNT          246 a          680 c          674 g          203 t
ORIGIN      
        1 gcccagcccg tcgggcagca gcgtccaggc gcccgccagc acgacggcgc cgaccggcag
       61 cgccggcagg aacacccagc gccagctcgc gtcctcgacc agccacgccg ccagcagcgg
      121 cccggccgcg ctcatgccga acagcagcag catgaaccac ttgagcgcgc gcggccgctc
      181 ggccgccggg aacagcagcg gcaccagcac ccggctgctg gtgaacagcg ccccgccgcc
      241 gaagccctgg accagccgcg cgagcgtgag cgaggcgagg ccgcccgagc ccgcgcacgc
      301 cgccgcgccg gccatgaaca gcgccagcgc gccggtcagg tagcgccggt agccgagatg
      361 gcgcgcgaag gtctgctgca acacgatcat cagcatgctg ccgaccgcat aggcggcctg
      421 cacctgcgcg aactcgcgcg gcgccgcgtc gatgccgccg acgacgtggc tcgcggcgaa
      481 cacgaacatc gcgttctcca tgaactccag ccccaccagc gcgcccagga gcgccatcag
      541 caggcgcttc ttgcgaagat gccagccgtg ggcgggatgc gcggacgcgg ccggggcggc
      601 ggcgcgcgtc atggggcgtc gggcccggac agcgtcgcgt gcacgcgcgt gagcgcccgc
      661 agcgtgcgac gcgtcgactc cgggcccagc ccggaccagg ccgccgcata cgccggatgc
      721 accgcctgcg ccgcgcgccg ggtcagcgcc ttgcccgcgg ccgtggactc cagctgcagg
      781 ctgcggcggt cggccgagtt gggtgcgcgc cgcaccaggc ccttcgcctc gagcccgtcg
      841 agcagccgcg tgacctgcgt gcgcgtggcg tccagcgtcg cgctcaggtc ggacgggcgc
      901 agcgcgtccg cgtcgtcgcc gacgagcacc agcgccaggt actcgcgcat gtcgatgccc
      961 agcggcgcca gcgcggcatc gatgcgcgac tgcagcaggt gcgcaacccg gaagacgagg
     1021 cgcgatgcgc gcacggcgtc ggccagctcc tcgggcttgc cggccagcag ccggtcgagg
     1081 cgggactcga agctcatgat gttccggtcg atattgtttc aaatgaaatc ataccgaacg
     1141 cgacgagtcg ccggcgggcg aacgaggccg gccccgcgcg ccatgcggga ttcggcccgc
     1201 cgcggggatt cgatcgaggc aggcgcgagc cgcgcgcggg tcagtcgccg ccgcccttgg
     1261 cgaggcgcgc ggccagcttc agcagcgtgg gcacgcgctt cctgccgtgc atcgcgcgca
     1321 cgcgcgcctt ggcggcgccg aggtcgatgc cggcgcaggt gacggtcagc ggcgcggcct
     1381 cgtcttcgcg gtgcacctcg aactgcgccg gcgtgccccc cgccatcgcg cccttggacg
     1441 cgccgatcac cggcaccttg ccgtcgagcg cgtcgaacag ccggcgcccg agcccgggcg
     1501 tgtcctgcgg gtcgaggtgg acggggtcgt ccatcacgat cgcgtcgggc tgcaggcggt
     1561 gatcctgcag cagctgcagc agtgcgggca gcgcgcgcag gtcggcctcg ccgcgcgccg
     1621 gcttcgggcc cgcgggtacg cgggccatga cggtgcgcgc ggcctcgacg tcgccccagt
     1681 cctcgaacgc cacgcccgcg gcggtcacgg tgtcgccgtc gaggcgatgg gcgaggacga
     1741 gcttcatggc gggctccggg gaggggtggc gggccgccat tgtggcgccg cgcggtcgat
     1801 cga
//
LOCUS       DASVLN010000060         6976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_164701, whole genome shotgun sequence.
ACCESSION   DASVLN010000060 DASVLN010000000
VERSION     DASVLN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164701"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="lnt"
                     /locus_tag="VF453_01300"
     CDS             <1..968
                     /gene="lnt"
                     /locus_tag="VF453_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX7686304.1"
                     /translation="PARRRRALLALAAPAVVVAGGAFLPQDFTRSTGTISVTLLQTDV
                     AQDDKFEDAHVRAALAWHTERLAAASGQLVVTPESSLPVLPVQIPQETWRSYEKPFDV
                     PGRAAIVGTFLGDDRIGYTNSVIGLDATHHMTDGSEYHYGKRHLLPFGEFVPPGFQWM
                     LDMMKVPIGNQARGTTTASMPVAGQRVRPLICYEDLFGEDFAASMVGPDAATLMANVT
                     NLAWFGKVEIQDQHLQFSRMRALEFQRGQVRATNTGATAIIDWHGDVTARLPPEIEGR
                     LDGRVEGRIGDTPYGRWLARWRLWPLWAAGVLALVAARVFVLRRD"
     gene            1045..1551
                     /gene="bamE"
                     /locus_tag="VF453_01305"
     CDS             1045..1551
                     /gene="bamE"
                     /locus_tag="VF453_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX7686305.1"
                     /translation="MRPSRSPLLAAAALGIGLAGCALQPYTPTDVHPGDSRDAVVAKM
                     GPPTATYTMPDGHLRLEYNHMPAGKHTYMIDFDAAGRVVKWENVLDENHFAAIRPGMT
                     SAEVLRLIGPPTYTWHYFRPTPANTWLYRFETPQRCILFEISFDLATGRVIDGDYPPD
                     PACPDEWM"
     gene            1630..2148
                     /locus_tag="VF453_01310"
     CDS             1630..2148
                     /locus_tag="VF453_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686306.1"
                     /translation="MIPSIRSRHAALAAVAALSVLCGCALRPYEPTRVHPGDTRAAVE
                     ATMGPPTAVYALADGRQRLEYNHQPAGLQTYMIDLGPDGRVASWEQVLDENHFATIAA
                     GMTEQDVLQRLGPPAERGRYAHPVESRTWTYRWQSPLPCVVFVISFDAATGRVLEPGS
                     YPPDRRCGPIRA"
     gene            2298..3218
                     /gene="glyQ"
                     /locus_tag="VF453_01315"
     CDS             2298..3218
                     /gene="glyQ"
                     /locus_tag="VF453_01315"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX7686307.1"
                     /translation="MLTFQQIILRLQDYWDKQGCALLQPYDMEVGAGTSHTATFLRAL
                     GPEPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPANILELYLGSLEALGF
                     DLKKNDVRFVEDDWENPTLGCWGLGWEVWLNGMEVTQFTYFQQVGGIDCKPITGEITY
                     GLERLAMYIQGKESLFDLEWTPGVSYRDVFHQNEVEQSTYNFEHSDVEFLLHAFGAHE
                     KMSKHLMAHQLALPAYEQLLKAGHTFNLLDARGAISVTERAAYIGRIRNLARAVAQSY
                     LESRARLGFPMAPRAWADEVVAQLDKKKAA"
     gene            3221..5332
                     /gene="glyS"
                     /locus_tag="VF453_01320"
     CDS             3221..5332
                     /gene="glyS"
                     /locus_tag="VF453_01320"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX7686308.1"
                     /translation="MSANDTKNLLVELVCEELPPKALKKLGESFAATLVASLHSAGLV
                     GANAVATSFASPRRLGVHVQGVAAKAADRALQQKLMPAAVAFDKDGNASPALLKKLAS
                     LGFGAEAVPTLKRLPDGKAESLFVDSTVAGASLADGLQKAIDETLAKLPIPKVMSYQL
                     ADGWSDVKFVRPAHRLVALHGADVVPVTALGLAADRVTQGHRFEAKAATIAVRDADSH
                     AAQLAEEGAVIASFAERRAEIVRQLADAAAKEGLKPIEDEALLDEVTALVERPNVLTC
                     RFEEDFLAVPQECLILTMKANQKYFPLLDAAGKLTNRFLVVSNIRPADPSAVIQGNER
                     VVRPRLADAKFFFDQDRKRALADRVPGLDKVVYHGKLGSQGARVERVRQIARAIAAEL
                     GDHGLVDKVDTAARLAKADLLTDMVGEFPELQGVMGGYYARHDRLGDEIADAIEDHYK
                     PRFAGDALPRNRTGLVVALADKLETLVGLFGVGQVPTGDKDPFALRRHALGVIRMLVE
                     AALPLDLDTLLALAVPAFGALVADPREAVAAFVFDRLAGSLREQGYSAHEVDAVLALA
                     PQRLAEVPRRLEAVRAFSALPEAEALAAANKRIANILKKSAEPVAAKADPALFAEAAE
                     GALWTDVAAVRRSADVLFASGDYTSSLKALAALRTPVDRFFDDVMVNADDPKVRANRL
                     GLLATLHGVMNRVADLSRLSS"
     gene            5404..5970
                     /gene="gmhB"
                     /locus_tag="VF453_01325"
     CDS             5404..5970
                     /gene="gmhB"
                     /locus_tag="VF453_01325"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX7686309.1"
                     /translation="MQAPKLVITGRDGILNVFRNDHVKAPEEWEPIPGALEAVARLNH
                     AGWHVVVATNQSGIGRGMIDMTTINAVHLHMMKLLGEKGGRIDAVFFCPHAATEQCEC
                     RKPAPGLMREIGERYGVDLEDVPMVCDTLRDLHAARAAGCVPHLVRTGRAAGVDDATL
                     AQWLQEVPGTAVHADMAAFVAHLVDAEH"
     gene            6031..6813
                     /locus_tag="VF453_01330"
     CDS             6031..6813
                     /locus_tag="VF453_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7686310.1"
                     /translation="MAKALSIVRSILFFLWMAVTVIPWGTVVVLCSPFLGADAIYTMC
                     VTWVRLAIDGSRFICGVKRHVVGMENLPGKDDPNAAVILASKHQSTWETFTFPSIMPH
                     PLAYVFKKELLYIPFFGWAMSRLDMIHIDRSRRTEAWNKVAQQGKRLSALGKWVIMFP
                     EGTRSPRGGQGTYKAGASRLAIAIGVPIVPIAVNSARCWPRKSFLLKPGVVTVSIGKP
                     IPSVGREPDELMREVEAWIEAEMRRLDPEAYAGAPAGASATA"
BASE COUNT         1019 a         2602 c         2393 g          962 t
ORIGIN      
        1 gcccggcccg gcgccgccgc gcgctgctgg cgctcgcggc gccggcggtc gtcgtcgccg
       61 ggggcgcctt cctgccgcag gacttcacgc gctcgaccgg cacgatcagc gtcacgctgc
      121 tgcagaccga cgtcgcccag gacgacaagt tcgaggatgc ccacgtccgc gccgcgctcg
      181 cctggcacac cgagcgcctg gccgccgcga gcgggcagct ggtcgtgacg ccggaatcct
      241 cgctgccggt gctgccggtg cagatcccgc aggagacctg gcgctcctac gagaagccgt
      301 tcgacgtccc gggccgcgcg gccatcgtcg gcaccttcct cggcgacgac cggatcggct
      361 acaccaactc ggtcatcggc ctggacgcca cgcaccacat gaccgacggc agcgagtacc
      421 actacggcaa gcgccacctg ctgcccttcg gcgagttcgt gccgcccggc ttccagtgga
      481 tgctggacat gatgaaggtg cccatcggca accaggcgcg cggcaccacg accgcgtcga
      541 tgccggtggc cggccagcgc gtgcggccgc tgatctgcta cgaggatctc ttcggcgagg
      601 acttcgcggc ctcgatggtc ggcccggacg cggcgacgct gatggccaac gtgaccaacc
      661 tcgcctggtt cggcaaggtc gagatccagg accagcacct gcagttctcg cgcatgcggg
      721 cactcgagtt ccagcgcggc caggtgcgcg ccaccaacac cggcgccacg gcgatcatcg
      781 actggcacgg cgacgtcacc gcgcgcctgc cgcccgagat cgaggggcgg ctcgacggcc
      841 gcgtcgaggg ccgcatcggc gacacgccct acggccgctg gctggcgcgg tggcggctct
      901 ggccgctgtg ggccgcgggc gtgctggcgc tcgtggcggc gcgggtgttc gtgctgcggc
      961 gggactgaaa tcccttccga tcgcccgccc cggcggcagg gccgtcgcgc gcggcggcgg
     1021 cgctttctgg catcctcggc gcgcatgcgc ccctcgcgat ccccgctcct cgccgccgcg
     1081 gccctgggca tcggcctggc cggctgcgcc ctccagccgt acacgcccac cgatgtccac
     1141 ccgggcgaca gccgcgacgc cgtcgtcgcg aagatgggcc cgccgaccgc cacctacacg
     1201 atgcccgacg gccacctgcg cctcgagtac aaccacatgc cggccggcaa gcacacctac
     1261 atgatcgact tcgacgccgc cggccgcgtc gtgaagtggg agaacgtcct cgacgagaac
     1321 cacttcgcgg cgatccggcc cgggatgacc tcggccgagg tgctgcgcct gatcgggccg
     1381 ccgacctaca cctggcacta tttccgcccg acgccggcca acacctggct ctaccgcttc
     1441 gagacgccgc agcgctgcat cctgttcgag atctcgttcg acctcgcgac cggccgcgtg
     1501 atcgacggcg actacccgcc cgacccggcc tgcccggacg agtggatgtg aggcccgcgc
     1561 gaccgcggcc gctatagtgc cgggcggccg ccgtggccgc gccagtccgg cacgccgccg
     1621 gcctccgcga tgattccctc gatccgatcc cggcacgccg cgctcgcggc cgtggcggcg
     1681 ctctccgtgc tttgcggctg cgcgctgcgt ccctatgagc cgacccgcgt gcatcccggc
     1741 gacacgcgcg ccgccgtcga ggcgaccatg ggcccgccga ccgccgtcta cgcgctggcc
     1801 gacggccgcc agcgcctcga atacaaccac cagcccgcgg gcctgcaaac gtacatgatc
     1861 gacctcgggc cggacggccg cgtcgcgtcg tgggagcagg tgctcgacga gaaccacttc
     1921 gcgacgatag cggccggcat gaccgagcag gacgtcctgc agcgcctggg cccgcccgcc
     1981 gagcgcgggc gctacgcgca tccggtggag tcgcggacct ggacgtaccg ctggcagtcg
     2041 ccgttgcctt gcgtcgtctt cgtcatctcg ttcgacgccg ccaccggtcg cgtgctcgag
     2101 ccgggaagct atccgcccga tcgacgctgc ggccccatcc gggcctgagc gggcgaccgc
     2161 gtcccggcga acgcccggac gccgcggcgc gtccgcaccg taaaatcgcc cgttcgaccg
     2221 tccgcagccc gccgctccca gccgcgcgtc cggacggcca gaccaccctc aggccgcccg
     2281 cggccgcatc cgacgccatg ctgaccttcc agcaaatcat tcttcgcctg caggactact
     2341 gggacaagca gggctgcgcg ctgctgcagc cctacgacat ggaggtcggc gcgggcacga
     2401 gccacaccgc gaccttcctg cgcgccctgg gcccggagcc gtggaaggcg gcctacgtgc
     2461 agccgagccg ccgccccaag gacggccgct acggcgagaa cccgaaccgc ctgcagcact
     2521 actaccagta ccaggtggtg ctcaagccgg cgcccgccaa catcctcgag ctgtacctcg
     2581 gctcgctgga ggcgctcggc ttcgacctga agaagaacga cgtccgcttc gtcgaggacg
     2641 actgggagaa ccccacgctc ggctgctggg gcctgggctg ggaggtgtgg ctcaacggca
     2701 tggaggtgac gcagttcacc tacttccagc aggtcggcgg catcgactgc aagccgatca
     2761 ccggcgagat cacctacggc ctcgagcgcc tggcgatgta catccagggc aaggaaagcc
     2821 tgttcgacct cgagtggacg cctggcgtga gctaccgcga cgtgttccac cagaacgagg
     2881 tcgagcagtc gacctacaac ttcgagcact cggacgtcga gttcctgctg cacgccttcg
     2941 gggcgcacga gaagatgtcc aagcacctga tggcgcacca gctcgcgctg cccgcctacg
     3001 agcagttgct caaggccggc cacaccttca acctgctcga cgcccgcggc gcgatcagcg
     3061 tgaccgagcg cgccgcctac atcggccgca tccgcaacct cgcgcgcgcc gtggcgcaga
     3121 gctacctcga gagccgcgcg cgcctcgggt tcccgatggc cccgagggcc tgggccgacg
     3181 aggtcgtcgc gcaactggac aagaagaagg cggcctgagc atgagcgcca acgacaccaa
     3241 gaacctcctc gtcgaactcg tctgcgagga actgcctccc aaggccctga agaagctggg
     3301 cgagtccttc gccgcgacgc tggtcgcgag cctgcactcg gccgggctgg tgggcgcgaa
     3361 cgcggtggcg acgtccttcg cctcgccgcg ccgcctgggc gtgcacgtcc agggcgtggc
     3421 cgccaaggcc gccgaccgcg cgctgcagca gaagctgatg ccggcggcgg tcgcgttcga
     3481 caaggacggc aacgcctcgc ccgcgctgct gaagaagctc gcgtcgctgg gcttcggcgc
     3541 cgaggccgtg ccgacgctca agcgcctgcc ggacggcaag gccgagagcc tgttcgtcga
     3601 ttcgaccgtc gccggcgcgt cgctggccga cggcctgcag aaggcgatcg acgagacgct
     3661 cgcgaagctg cccatcccga aggtcatgag ctaccagctc gccgacggct ggagcgacgt
     3721 gaagttcgtg cgcccggccc atcgcctcgt cgcgctgcac ggcgcggacg tcgtgccggt
     3781 cacggcgctc ggcctggccg ccgaccgcgt cacccagggg catcgcttcg aggccaaggc
     3841 cgcgaccatc gccgtccgcg acgccgacag ccacgccgcc cagctcgccg aggagggcgc
     3901 ggtcatcgcg tccttcgccg agcgccgcgc cgagatcgtg cgccagctcg ccgacgcggc
     3961 cgcgaaggaa ggcctgaagc cgatcgagga cgaggcgctg ctcgacgagg tgaccgcgct
     4021 ggtcgagcgc ccgaacgtgc tgacctgccg cttcgaggag gacttcctcg ccgtgccgca
     4081 ggaatgcctg atcctcacga tgaaggccaa ccagaagtac ttcccgctgc tggacgcggc
     4141 cggcaagctc acgaatcgct tcctcgtcgt cagcaacatc cggcccgcgg acccatcggc
     4201 ggtgatccag ggcaacgagc gcgtcgtgcg cccgcgactg gccgacgcga agttcttctt
     4261 cgaccaggac cgcaagcgcg cgctcgccga ccgcgtgccg ggcctggaca aggtcgtcta
     4321 ccacggcaag ctcggcagcc agggcgcgcg cgtcgagcgg gtgcgccaga tcgcgcgtgc
     4381 gatcgcggcg gaactcggcg accacggcct cgtggacaag gtcgacaccg ccgcgcgcct
     4441 cgccaaggcc gacctgctga ccgacatggt cggcgagttc cccgagctgc agggcgtgat
     4501 gggcggctac tacgcgcgcc acgacaggct aggcgacgag atcgccgacg cgatcgagga
     4561 tcactacaag ccgcgcttcg ccggcgacgc gctgccgcgc aaccgcacgg gcctggtcgt
     4621 cgcgctggcc gacaagctgg agacgctggt cggtctgttc ggcgtcggcc aggtgcccac
     4681 cggcgacaag gatcctttcg cgctgcgccg ccacgcgctg ggcgtgatcc gcatgctggt
     4741 cgaggcggcg ctgccgctgg acctcgacac gctgctggcg ctggccgtgc cggcgttcgg
     4801 cgcgctggtg gccgatccgc gcgaggcggt ggccgcgttc gtgttcgatc gcctggccgg
     4861 atcgctgcgc gagcagggtt actcggcgca cgaggtcgac gcggtgctgg cgctcgcgcc
     4921 gcagcggctg gccgaggtgc cgcgccgcct cgaggccgtg cgcgccttct ccgcgctgcc
     4981 cgaggccgag gcgctggccg ccgcgaacaa gcgcatcgcc aacatcctga agaagagcgc
     5041 cgagcccgtc gcggcgaagg ccgacccggc gctgttcgcc gaggccgccg agggcgcgct
     5101 gtggaccgac gtcgcggcgg tgcgccgttc ggccgacgtg ctgttcgcgt ccggcgacta
     5161 cacgagcagc ctgaaggcgc tcgccgcgct gcgcacgccg gtcgaccgct tcttcgacga
     5221 cgtgatggtc aacgccgacg atccgaaggt gcgcgcgaac cggctgggcc tgctcgcgac
     5281 gctgcacggc gtgatgaacc gggtcgcgga tctttcgcgc ctgagttcat gacgactccc
     5341 ctcgtcgcgc gcgtcgcgct cgtcgcgccc cgaggcgtat tccctttctg acccggagtc
     5401 cgcatgcaag ctcccaagtt ggtcatcacc ggccgcgacg gcatcctcaa cgtgttccgc
     5461 aacgaccacg tgaaggcgcc cgaggagtgg gagccgatcc cgggggcgct ggaggccgtc
     5521 gcgcggctga accacgcggg ctggcatgtc gtcgtggcga ccaaccagtc cggcatcggg
     5581 cgcgggatga tcgacatgac gacgatcaac gccgtgcacc tgcacatgat gaagctgctg
     5641 ggcgagaagg gcgggcgcat cgacgccgtc ttcttctgcc cgcacgcggc caccgagcag
     5701 tgcgagtgcc gcaagcccgc gccggggctg atgcgcgaga tcggcgagcg ctatggcgtc
     5761 gacctcgagg acgtgcccat ggtctgcgac acgctgcgcg acctgcacgc cgcgcgcgcc
     5821 gccggctgcg tgccgcacct cgtgcgcacc ggccgcgcgg ccggcgtgga cgacgcgacg
     5881 ctcgcgcaat ggctgcagga ggtgcccggc accgccgtcc acgccgacat ggcggccttc
     5941 gtggcgcacc tggtcgacgc cgagcactga tcctcccggg ccgcggcccc gcgcgcgcgg
     6001 ccctttcctc accgacgaca agaacaacgc atggccaagg ccctctccat cgtccgctcg
     6061 atcctgttct tcctgtggat ggcggtcacc gtcatcccgt ggggcacggt ggtcgtgctg
     6121 tgctcgccct tcctcggcgc cgacgcgatc tacacgatgt gcgtgacctg ggtgcggctc
     6181 gcgatcgacg gctcgcgctt catctgcggc gtcaagcggc acgtggtcgg catggagaac
     6241 ctgcccggca aggacgaccc gaacgccgcg gtgatcctcg cctccaagca ccagtcgacc
     6301 tgggagacct tcacgttccc gtcgatcatg ccccacccgc tcgcctacgt gttcaagaag
     6361 gagctgctgt acatcccctt cttcggctgg gcgatgtcgc ggctggacat gatccacatc
     6421 gaccgcagca ggcgcaccga ggcctggaac aaggtggcgc agcagggcaa gcggctcagt
     6481 gcgctgggca agtgggtgat catgttcccg gaaggcacgc gcagcccgcg cggcggccag
     6541 ggcacctaca aggcgggcgc gtcgcgcctg gccatcgcca tcggcgtgcc catcgtgccg
     6601 atcgcggtca actcggcgcg ctgctggccg cgcaagagct tcctgctgaa gccgggcgtg
     6661 gtgacggtgt cgatcggcaa gcccatcccg tccgtcggac gggagcccga cgagctgatg
     6721 cgcgaggtcg aggcctggat cgaggccgag atgcgccgcc tcgaccccga ggcctatgcc
     6781 ggcgcgcctg caggcgcctc cgcgaccgcc tgaaggctgt cgcgccgggt gccggccggt
     6841 agaattcgcg gccatggccc gatccgccac gatcgacgac tccgcgcaga tgtccctgtt
     6901 cgatgcctcg ccggcggcgg gcgaggcggc ggtgcccgtc gtcgcgccgt cagcgccggt
     6961 cgccgccgcg gacgac
//
LOCUS       DASVLN010000061         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_486781, whole genome shotgun sequence.
ACCESSION   DASVLN010000061 DASVLN010000000
VERSION     DASVLN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486781"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF453_01335"
     CDS             <1..502
                     /locus_tag="VF453_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7686311.1"
                     /translation="QVRTEQANVEVAAAQVATAQAALKAADIQLGYTHVRAPISGRVA
                     KRSVDVGNVVAIGQPLLAIVSDDLWVTANYKETQLKKMRLHQPVTIEVDAVPGVKFAG
                     HVDSLQRATGAYFSMLPSENATGNYVKVTQRVPVKIVFDHPEDLKRYPIGPGMSVKPS
                     VDLDAR"
     gene            513..2042
                     /locus_tag="VF453_01340"
     CDS             513..2042
                     /locus_tag="VF453_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7686312.1"
                     /translation="MASASLPRSAAGDHNPWLIAIVVSLATFMEVLDTTIANVSLRHI
                     AGGLGASQDEATWILTSYLISNAIILPVSGWLATLVGRKKFYMTCVALFTGSSFMCAL
                     SPNLATMIFFRVLQGLGGGGLSPTEQSIFADTFPPAQRAKAFAFYGVTVIVAPAIGPL
                     LGGWLTDSFSWHWIFLINLPVGVAALTLIWLLVDEPPAVVEDTREFRRNFQPDWLGFL
                     LVAATFGCLQVVLDRFQEDDGFASDKIVVFFIVFVCSAILLVWWELQHPQPVFDVRLF
                     RFGSFGAANIVMLLVGFTLFSTTQLIPQFAQNLLQYDALKAGESLALGGLAAAVMMPL
                     AGIAAGKLPPKVLVGAGLLATGLALIHTSYLNGLIGFWDLSLTRVYQSWCLPFLFVSL
                     TTAGYVGIPPDRNNEASAIINLSRNLGGSIGVAAATTELAWRVQFHHARIGEQISTIG
                     ARGHAFAQQGLAATQQAVNTQAQILSYLDIFYIFGIGALCVVPVAFLLKTPPAGAAHG
                     H"
BASE COUNT          299 a          778 c          714 g          358 t
ORIGIN      
        1 gcaggtgcgc acggagcagg ccaacgtcga ggtcgcggcc gcgcaggtcg ccacggcgca
       61 ggcggcgctc aaggcggccg acatccagct cggctacacg cacgtgcgcg cgccgatctc
      121 ggggcgcgtg gccaagcgct cggtggacgt cggcaacgtg gtggcgatcg gccagccgct
      181 gctggccatc gtcagcgacg acctctgggt cacggccaac tacaaggaga cgcagctcaa
      241 gaagatgcgg ctgcaccagc ccgtgacgat cgaggtcgac gcggtgccgg gcgtgaagtt
      301 cgcgggccac gtcgacagcc tgcagcgcgc gaccggtgcc tacttcagca tgctgccgtc
      361 cgagaacgcg accggcaact acgtgaaggt gacgcagcgc gtgccggtga agatcgtctt
      421 cgaccatccc gaggacctga agcgctaccc gatcgggccg ggcatgtcgg tcaagcccag
      481 cgtcgacctc gacgcgcgct gaagccgccc gcatggcctc cgcctcgctc ccgcgctcgg
      541 ccgccggcga ccacaacccg tggctgatcg ccatcgtggt ctcgctcgcg accttcatgg
      601 aggtgctcga cacgacgatc gccaacgtct cgctgcgcca catcgccggc ggcctgggcg
      661 cgagccagga cgaggcgact tggatcctca cgagctacct catctccaat gcgatcatcc
      721 tgccggtcag cggctggctc gcgacgctgg tcgggcgcaa gaagttctac atgacctgcg
      781 tcgccctgtt cacgggcagc tcgttcatgt gcgcgctgtc gcccaacctc gcgacgatga
      841 tcttcttccg cgtcctgcag ggcctgggcg gcggcgggct gtcgccgacg gagcagtcca
      901 tcttcgccga caccttcccg ccggcgcagc gcgccaaggc cttcgcgttc tacggcgtga
      961 ccgtcatcgt ggcgcccgcg atcgggccgc tgctgggcgg ctggctcacc gacagcttct
     1021 cctggcactg gatcttcctg atcaacctgc ccgtgggcgt cgcggcgctg acgctgatct
     1081 ggctgctggt cgacgagccg cccgccgtcg tcgaggacac gcgcgagttc cggcgcaact
     1141 tccagcccga ctggctgggc ttcctgctcg tggccgcgac cttcggctgc ctgcaggtgg
     1201 tgctcgatcg cttccaggag gacgacggct tcgcgtcgga caagatcgtc gtgttcttca
     1261 tcgtcttcgt gtgctcggcg atcctgctgg tgtggtggga gctgcagcac ccgcagccgg
     1321 tgttcgacgt gcggctgttc aggttcggct cgttcggggc cgcgaacatc gtgatgctgc
     1381 tggtcggctt cacgctcttc agcaccacgc agctgatccc gcagttcgcg cagaacctgc
     1441 tgcagtacga cgcgctgaag gcgggcgagt cgctcgcgct gggcggcctg gcggcggcgg
     1501 tcatgatgcc gctcgcgggc atcgccgccg gcaagctgcc acccaaggtg ctcgtgggcg
     1561 cggggctcct cgcgacggga ctcgccctga tccacacgtc ctacctcaac gggttgatcg
     1621 gcttctggga tctctcgctg acgcgcgtgt accagagctg gtgcctgccc ttcctcttcg
     1681 tctcgctgac gaccgcgggc tacgtcggca tcccgccgga tcgcaacaac gaggcctcgg
     1741 ccatcatcaa cctctcgcgc aacctgggcg gcagcatcgg cgtggcggcg gccaccacgg
     1801 agctcgcctg gcgcgtccag ttccaccacg cccgcatcgg cgagcagatc tcgacgatcg
     1861 gcgcgcgcgg ccacgcgttc gcgcagcagg gcctggcggc gacgcagcag gccgtcaaca
     1921 cccaggcgca gatcctgagc tacctggaca tcttctacat cttcggcatc ggcgcgctgt
     1981 gcgtcgtgcc ggtcgcgttc cttctcaaga cgccgcccgc gggagccgcg catggccact
     2041 gatcgcatcc gccccgatac ccccccaagg catgtacccg cccgcctgcg cgcgccgctg
     2101 tcggcgctcg cggcggccgc gctgctggcg ggctgcatga ccgtcggcc
//
LOCUS       DASVLN010000062         9699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_379191, whole genome shotgun sequence.
ACCESSION   DASVLN010000062 DASVLN010000000
VERSION     DASVLN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379191"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VF453_01345"
     CDS             complement(<1..338)
                     /locus_tag="VF453_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686313.1"
                     /translation="MTPPNAGASLPSPRAAAARAVAFLAAVALPILWSKSLPPSATHL
                     NEIVSVAGWGLLMCVFPAAASLRASLRAAWPVAAALAVCALACAWTLVHDAPSTDPWL
                     PALAMMGFGIG"
     gene            complement(386..1975)
                     /locus_tag="VF453_01350"
     CDS             complement(386..1975)
                     /locus_tag="VF453_01350"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7686314.1"
                     /translation="MENVQTVIIGAGPAGVTAGLELVRAGRRDVLVLEATDDIGGISR
                     TVNHKGNRIDIGGHRFFSKSDWVMNWWRELMPVAMPPGAPPEAEFRLAYQGASRLLGA
                     EHVHATEGDREVMLLRNRLSRIYFNGAFFSYPLKPGFDMVTKLGVAKCSRFALSYARS
                     HLSPIAPEQSLEDFFINRFGRALYLQFFKSYTEKVWGVPCNEISAAWGAQRVKSLNVG
                     NMVAHALKKLVPGGRKGGDEQTSLIENFLYPKYGPGQMWEVAAQRFEQGGGRILRNAK
                     VVGIEREGDRVKAVTARVAGGDVLRVEAGHVVSTMPIKDLTLAMQADVPAAIADIGGR
                     LEYRDFITVGLLYRKLRRTAETLPSKTNLVADNWIYIQDAGVKVGRLQVFNNWSPYMV
                     ADPDTVWVGLEFFARDDDELWGLSDDALKSLALREMRELKLADEADALDAIVIRQPKA
                     YPGYFGDAYTHFDELRGWLDGIRNLYLVGRNGMHRYNNQDHSMLSARYAVEAILAGGG
                     DKSAIWNVNIDDDYHEEADAK"
     gene            complement(2013..4025)
                     /locus_tag="VF453_01355"
     CDS             complement(2013..4025)
                     /locus_tag="VF453_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686315.1"
                     /translation="MNTPVSTLTAGGIRADESSAMTSSAEPHDLATSSATTAMPIDRE
                     RAALRVPTWAGLLLIAALWLVVRPYRGVRHDAVLYLGQTLSRLMPDTIGKDLFFAYGS
                     QDRYSIFSPLMAPLVRWLGVGTSEIVVLAISEVLFLVGCWSLVRSLPSRFLRWCSMLS
                     LASVSHVYGAFGMVAFAEPFLSARSLSEPFVVFALAFLARDRLGAAVAFMLMAIVMHP
                     LIALPGLVAAWFYLLREDRRWFWALTLVAVPLVAGAAGIAPFDALWHRYDDEWLAAVR
                     TADPQVFMANMTRLDTSPMIFDVGVLLVVGAQMRPSPMGRLILAVLLSTATCFVIWGV
                     GSDLFHNVLLSQLQLWRAHWLTHLMALLMLPVLLTNLWDRGAVGRWAACAVVFAMLAV
                     TGNIGAGWLCIVWAGLALLALHSGTPVSAGVLRLATIGSVAGSLLITAIMFRSTMYVV
                     SAASDRFGGVGLVQVVVGLTLGAATLGFVVLRGLAARGLPRAIAMAAVVGLAAAGMAS
                     YDQRSDWQRYVENGLEETALPFAGELAPSAKVYWDGSLIEAWMLMHRGEYYSVEQGAG
                     LLFNRATAAAYMARKRQFAGIDAAREICGTISAFTGANTDDPDSCPPSVESIKAVCAT
                     PGGPDALVFRAGSSGKSLGATAMWTYAPTDPVHRRDYRLYDCHKLH"
     gene            complement(4022..4423)
                     /locus_tag="VF453_01360"
     CDS             complement(4022..4423)
                     /locus_tag="VF453_01360"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX7686316.1"
                     /translation="MIEFLRYLAASALAFGIDLGLYRLGLSLGLGYALSACLGFTLGL
                     AVAYGLSVRWAFHVRGVRDARAEFAIFALVGVAGLLLTEGLLWLQIDLLHLHRVVAKV
                     DAAGVVFLFNFSVRKMLLFTRRNALAAGRIA"
     gene            complement(4420..6408)
                     /locus_tag="VF453_01365"
     CDS             complement(4420..6408)
                     /locus_tag="VF453_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686317.1"
                     /translation="MSVLAPSGPSMQEAPPAAQVRVAPFIVPTWYGALLVGALLLANQ
                     PYRGVRHDAVLYLGQALIRLDPSWWRSDVYFAFGSQDQYSVFSRLFALPVRAFGIPVA
                     EMAALTIGRLATFCALYLLVDRWPPLRRWLALACVAGVVHYYGGKTFSAIEPFVTART
                     FAEPLSLFAIGAVLRGRWTLGALSLAAAFIVHPLIALPTALILWVYLVVAEGRRWIGV
                     GAVVAALVLALGAAGVMPFDGLLRRYDNDWFTMAGFLTSTAFVSEWSARAYVAILGYA
                     VIVWAATWHRRDPLARLSRIAVVLGPLCCAASFWIADWEHNRLLTQLQFWRTIWIGEV
                     LSALWLPTLVLDQWARGAVGRVAAAAVCAGWIVVQDDLGLYGWAVLLWIALWLMLSLR
                     DAVIERRVLRLAFAATVLAGLMAVGQHCWDALYQLRADMRGFALTYPASIPFRVPMLT
                     VSTIGLLLVAWQTPRWRPACAAAAIALAVISIWQTDQRDEFTRFVESATPAQRPFQSD
                     IPPTARVYWDDDEVAPVWLLLHRAAFGEPAQFSGALFTRQVALTGRDIWSFLDYNSQR
                     LGHCAELQSWTSRDYVFQDCIVPLDRMRTFCAGSLHPDFIVSKMLYPLPQRSQWTFRS
                     PDGGEPVNYYLYACSAIRAAAPPPAAFSASPPRPKAIP"
     gene            complement(6621..7082)
                     /locus_tag="VF453_01370"
     CDS             complement(6621..7082)
                     /locus_tag="VF453_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEX7686318.1"
                     /translation="MKRTVQKGFTLIELMIVVAIIGILAAVALPAYKTYTIKAKVSEV
                     ILAASQCRTTVAEVYQTASSLPAANGWGCETTSASSKYVASVTTSGSGQITVLTASNS
                     LVPTEIRSTTITLTPTDATGGALTGVGQQVGGFTCAPGNMDTKYLPGSCRG"
     gene            complement(7785..8567)
                     /locus_tag="VF453_01375"
     CDS             complement(7785..8567)
                     /locus_tag="VF453_01375"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HEX7686319.1"
                     /translation="MKIQVLGCSGSIARDCRTTSFLLDDDVLIDAGTGVGDLSLDALE
                     RIDHILLTHSHLDHVLGVPLLADSVLRRRQARGAAPIEVHALPQTLDVLRAHVFNNFV
                     WPDFTRLPSASAPVLRFVPFELGDTLVLGGRRIEVLPAAHSVPACGYAIHAATPGRGA
                     WVFSGDTGPNPAFWRRIAQFPVAHVVVETAFSEAESALASASGHHCPSSLAAELAGAP
                     ADARIYITHIKPGEIAAVMTGLRELKQERPLMELLRGDVFDV"
     gene            complement(8575..>9699)
                     /locus_tag="VF453_01380"
     CDS             complement(8575..>9699)
                     /locus_tag="VF453_01380"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7686320.1"
                     /translation="GPAAVLALAGILAALVAGDALALALRGWALPVAASLTGALLLGG
                     GGVVANYLSEWHSRRSLARLFGHYLPPERVRAMASDPDRFLDVASSAENRELTILFCD
                     LRGFTTMSETLAPERLREVLNLYFSRMSEIIHAHGGTLDKFIGDAVMAFWGAPLADPQ
                     HAAHAVRAALAMAAAVDPLNAELAARGLPSLAPCMGLASGVVCVGDLGSALRRSYTAV
                     GDGVNLAARVESATRDYGLALLVADATRAAAGELPGCAWIEVDEVAVKGRAQLVTLYV
                     PLPLPESGDAAEIARFHEQLGLWRLARESLRGHHDDSSPPASMPSARAVATTRLTELL
                     EARSLPPPLRALATRQLARLANRTGNPPAACEDPSTPPTA"
BASE COUNT         1717 a         3265 c         3409 g         1308 t
ORIGIN      
        1 ccgatcccga agcccatcat cgcgagcgcg ggcaaccagg ggtcggtcga gggcgcgtcg
       61 tgcaccagcg tccaggcaca ggcgagcgcg cacaccgcga gcgccgcggc caccggccag
      121 gccgcgcgca ggctcgcccg caggctggcg gcggcgggaa agacgcacat caggaggccc
      181 caccccgcca ccgacacgat ctcgttgagg tgcgtggccg acggcggcag cgacttgctc
      241 cagaggatcg gcagcgcgac ggcggccagg aacgcgaccg cccgcgccgc cgcggcacgc
      301 ggggacggaa gcgaggcgcc ggcgttcggg ggcgtcatcg ggggtcggtc gggaatccgc
      361 ggcgcgcggc gcgaaggccg gccgcctact tggcgtccgc ttcctcgtgg tagtcgtcgt
      421 cgatgttgac gttccagatg gccgacttgt cgccgccccc ggcgaggatc gcctcgaccg
      481 cgtagcgcgc ggacagcatg gagtgatcct ggttgttgta gcgatgcatg ccgttgcggc
      541 cgaccaggta gaggttgcgg atgccgtcga gccagccgcg cagctcgtcg aagtgcgtgt
      601 aggcgtcacc gaagtagccc gggtacgcct tgggctggcg gatcacgatg gcgtcgagcg
      661 cgtcggcctc gtcggccagc ttgagctcgc gcatctcgcg cagcgcgagc gacttcagcg
      721 cgtcgtcgga caggccccag agctcgtcgt cgtcccgcgc gaagaattcg agcccgaccc
      781 agacggtgtc cgggtcggcg accatgtagg ggctccagtt gttgaagacc tgcagccggc
      841 cgaccttgac gcccgcgtcc tggatgtaga tccaattgtc ggccacgagg ttggtcttcg
      901 agggcagcgt ctcggccgtg cggcgcaact tgcggtacag caggccgacg gtgatgaagt
      961 cccgatactc cagccgaccg ccgatgtcgg cgatcgccgc gggcacgtcc gcctgcatcg
     1021 cgagcgtcag gtccttgatc ggcatcgtcg agacgacgtg gcccgcctcg acgcgcagca
     1081 cgtcgccgcc ggccacgcgg gccgtcaccg ccttgacgcg gtcgccctcg cgctcgatgc
     1141 cgaccacctt cgcgttgcgc aggatccggc cgccgccctg ctcgaagcgc tgcgccgcca
     1201 cttcccacat ctggcccggg ccgtatttcg ggtagaggaa gttctcgatc agcgaggtct
     1261 gctcgtcgcc gcccttcctg ccgccgggca cgagcttctt cagcgcgtgg gcgaccatgt
     1321 tgccgacgtt caggctcttg acgcgctggg cgccccaggc cgcgctgatc tcgttgcagg
     1381 gcacgcccca caccttttcg gtgtacgact tgaagaactg caggtacagc gcgcggccga
     1441 agcggttgat gaagaagtcc tcgagcgact gctccggcgc gatcggcgac aagtgcgagc
     1501 gcgcgtagct cagcgcgaac cggctgcact tggccacgcc cagcttcgtc accatgtcga
     1561 agcccggctt gagcggatag ctgaagaacg cgccgttgaa atagatgcgc gagaggcggt
     1621 tgcgcagcag catcacctcg cgatcgccct cggtcgcgtg cacgtgctcg gcgccgagca
     1681 ggcgcgacgc gccctggtag gcgaggcgga actcggcctc cggcggcgcg cccggcggca
     1741 tcgccaccgg catcagctcg cgccaccagt tcatcaccca gtccgacttc gagaagaagc
     1801 ggtggccgcc gatgtcgatg cggttgccct tgtggttgac ggtgcgcgag atgccgccga
     1861 tgtcgtccgt cgcctccagc acgaggacgt cgcgccggcc cgcgcgcacg agttcgaggc
     1921 cggcggtcac gcccgcgggg ccggcgccga tgatgacggt ttgcacgttt tccatggagt
     1981 cgggatcggg ttcgcggtcg cgcgcacggc gctcagtgca acttgtggca gtcgtagagg
     2041 cggtagtcgc gacgatgcac cggatcggtc ggcgcgtacg tccacatcgc cgtggcaccg
     2101 agcgacttgc cgctcgagcc cgcgcggaac acgagcgcgt ccggcccgcc gggcgtggcg
     2161 cacaccgcct tgatgctttc gacggacggc gggcagctgt cgggatcgtc ggtattggcg
     2221 cccgtgaagg cggagattgt cccgcagatc tcgcgcgccg catcgatgcc cgcgaactgg
     2281 cgcttgcgcg ccatgtaggc cgcggcggtc gcacggttga acagcaggcc ggccccctgc
     2341 tcgaccgagt agtactcgcc tcgatgcatc agcatccagg cctcgatcag cgatccgtcc
     2401 caatacacct tggcggacgg cgcgagctca cctgcgaagg gcaacgcggt ctcctccagt
     2461 ccgttctcaa cgtagcgctg ccagtctgag cgctggtcgt aggaggccat gccagccgcc
     2521 gccaggccga ccacggcggc catcgcaatg gcacgcggca gtcccctcgc ggcaagcccg
     2581 cgcagcacga cgaagccgag cgttgcggca ccaagcgtca gccccacgac cacctgcacc
     2641 agcccgacgc cgccgaagcg gtcggatgcg gcgctaacga cgtacatcgt cgaacggaac
     2701 atgatggcgg tgatgagcag agatccggcc accgatccga tcgtcgcgag cctcaacacc
     2761 cccgccgaca ccggcgtgcc gctgtgcagc gccagcagcg ccagccccgc ccagacgatg
     2821 cacagccagc ccgcgccgat gttgccagtg accgccagca tcgcgaacac gaccgcgcat
     2881 gccgcccagc ggccgacggc gccgcgatcc cacaggttcg tcagcagcac gggcagcatc
     2941 agaagtgcca tcaggtgcgt cagccagtgc gcgcgccaca gttgcagctg ggacagcagg
     3001 acgttgtgga agaggtcgct gccgacgccc cagatcacga agcaggtggc ggtgctcagg
     3061 agcacggcca ggatcagccg ccccatcggc gacggacgca tttgcgcgcc gacgacgagc
     3121 aggacgccca cgtcgaagat catgggactc gtgtcgagcc tcgtcatgtt cgccatgaat
     3181 acctgcggat cggcggtccg cacggcagcg agccattcgt cgtcgtagcg gtgccagagc
     3241 gcgtcgaacg gcgcgatgcc ggcggcgcct gccaccagcg gcacggccac gagggtcagc
     3301 gcccagaacc aacggcgatc ctcccgcagg agatagaacc acgcggcgac caggcccggc
     3361 agggcgatca agggatgcat gacgatcgcc atcagcatga acgcgacggc cgcgcccagc
     3421 cggtcgcgcg caaggaaggc cagcgcgaac acgacgaacg gctcgctcaa tgaccgtgcg
     3481 ctcaggaacg gctcggcgaa ggcgaccatg ccgaacgcgc cgtagacatg cgagacggag
     3541 gcgagcgaca gcatcgagca ccagcgcagg aatcgcgacg gcagcgagcg gacgagcgac
     3601 cagcagccaa ccaagaacag cacttcggag atcgcgagga cgacgatttc ggaagtgccg
     3661 acgcccagcc atcgcacgag cggcgccatg aggggcgaga agatggagta gcgatcctgc
     3721 gagccgtagg cgaaaaagag atccttaccg atcgtgtcgg gcatcaggcg gcttagcgtc
     3781 tgtccgaggt agagcacggc atcatggcgc acgccgcgat acggccgcac gacgagccac
     3841 agggcggcga tgagcagcag tccggcccag gtcggcacgc gcaacgccgc gcgttcgcgg
     3901 tcgatgggca tcgcggtcgt ggccgacgag gtcgcgagat cgtggggctc ggcggacgaa
     3961 gtcatggcgg aggattcgtc cgcgcggatg ccgccggccg tcagggtgga aacgggcgtg
     4021 ttcatgcaat gcgccccgcg gccagcgcat tgcgccgcgt gaacaacagc atcttgcgca
     4081 cgctgaagtt gaacaggaac accacgccgg cggcgtcgac cttggccacg acgcggtgga
     4141 ggtgcagcag gtcgatctgt agccagagca ggccttcggt cagcagcaat ccggcgacgc
     4201 cgacgagcgc gaagatcgcg aactccgcgc gcgcatcccg gacgccgcgc acgtggaagg
     4261 cccagcgcac gctcaggccg tacgccacgg caagccccag cgtgaagccg aggcacgcgg
     4321 acagcgcgta gcctaggccc agcgacagcc cgagtcgata gagcccgaga tcgatgccga
     4381 aggccagcgc actggccgcg agatagcgga gaaattcgat catgggatgg ccttcggacg
     4441 tggcggggag gcggagaacg cggcaggcgg cggcgctgcc gcgcgtatgg cactgcaagc
     4501 gtagaggtag tagttcaccg gctcgccgcc gtcgggcgac cggaacgtcc actgcgaccg
     4561 ctgcggcagc ggatagagca tcttgctgac gatgaagtcc gggtgcaggc tgcccgcgca
     4621 gaaggtgcgc atgcggtcta gaggcacgat acagtcctgg aacacgtagt cgcgggaggt
     4681 ccaggattgc agttcggcgc agtggccgag ccgctgggag ttgtagtcga ggaaactcca
     4741 gatgtcgcgg cccgtcaggg ccacctggcg agtgaagagg gccccggaga attgcgccgg
     4801 ctctccgaac gcggcgcgat ggagcagcag ccacaccggc gcgacttcat cgtcatccca
     4861 gtaaacgcgg gcagtgggcg ggatgtcgga ctgaaatggc cgttgggccg gcgtcgccga
     4921 ctcaacgaag cgagtgaatt catctcgctg atccgtctgc cagatcgaga tcaccgccag
     4981 cgcgatggcc gcggcggcac aggcgggccg ccagcgcggc gtctgccatg ccacgagcag
     5041 caggccaatg gtcgacaccg tcagcatcgg cacgcgaaac ggaatcgacg ccgggtaggt
     5101 cagcgcaaag ccgcgcatgt ccgcgcgcag ctggtacagc gcatcccagc agtgctgccc
     5161 caccgccatc aggcccgcca gcaccgtggc ggcgaacgca agtcgaagga cgcggcgctc
     5221 gatcaccgcg tcacgcaggc ttaacatgag ccacagcgca atccagagca gcacggccca
     5281 gccgtagagg ccgaggtcat cctgtaccac gatccatccc gcacagaccg ccgccgcggc
     5341 gacgcgcccc acggcgccac gcgcccactg gtcgaggacg agggttggca gccacagcgc
     5401 cgatagcacc tctccgatcc agatcgtccg ccagaattgc agctgggtca gcaggcggtt
     5461 gtgctcccag tccgcgatcc agaacgacgc cgcgcagcac agcgggccca gcacgacggc
     5521 gatgcgcgag aggcgcgcca gcgggtccct ccgatgccac gtcgccgccc acacgatcac
     5581 tgcgtagccg aggatcgcaa cgtaggcgcg ggcggaccac tcgctgacga aggcggtcga
     5641 cgtgaggaag cccgccatgg tgaaccaatc gttgtcgtag cgtcgcagca agccgtcgaa
     5701 gggcatgaca ccggcggcgc cgagggcaag gacgagcgcc gcaacaactg cgcccacccc
     5761 tatccatcgc cgcccctcgg caacgacgag atagacccac aggatcaggg ctgtcgggag
     5821 agcgatcaag ggatgtacga tgaacgctgc agcaagcgac agggcaccca gggtccaacg
     5881 gccgcgcagc accgccccaa tggcgaaaag cgacagcggc tcggcgaatg tgcgcgcagt
     5941 gacaaacggt tcgatggcgg aaaaggtctt tccgccgtag tagtgcacga cgcccgcgac
     6001 acatgcgagc gccagccagc ggcgcaacgg cggccagcga tccaccagca ggtagagcgc
     6061 acagaacgtg gccagccggc caatcgtaag cgcggccatc tcggccacag gaatgccaaa
     6121 tgcgcgcacc ggcagcgcga acagccgcga gaagacgctg tactgatcct gggagccaaa
     6181 ggcgaaatag acgtcactgc gccaccaact gggatcgagc cgaatcaagg cctggcccag
     6241 gtagagcacc gcatcgtggc gaacaccgcg atagggctgg ttcgccagca gcaacgcgcc
     6301 gaccagcaag gccccgtacc acgtcggcac gatgaacggc gcaacacgta cctgcgcggc
     6361 aggcggcgct tcctgcatcg acgggcccga aggcgcgagg acgctcatcc cgttacccca
     6421 ggatgacgga gcgcacagat tgcaacgctg acaaaaggga acgaggtcat cgggaagctt
     6481 cgaccggatc ggggccgcca gacaggtcga cgactgcgag attgtccgga tccggccggt
     6541 aggtcggatt cagaaaaaaa ggcgcctttc ggcgcccttt cgcggcggcg aatgcgccgc
     6601 ctgtccttgc tggatcaagt tcagccgcgg caagagcccg gcaggtactt ggtgtccatg
     6661 ttgcccggag cgcaggtgaa gccaccgact tgctggccga cacccgtcag ggcgccaccg
     6721 gtggcatccg tcggcgtcag cgtgatcgtc gtgctgcgga tttccgtcgg caccaggctg
     6781 ttgctggcgg tgagaacggt gatctggcca gaaccgctgg tcgtgacgga ggcgacgtac
     6841 ttgctgctcg ccgacgtggt ctcgcaaccc cagccattgg cggcgggcag cgacgacgcg
     6901 gtctgataga cctccgcgac ggtcgtacgg cactggctgg cggccaggat gacttccgag
     6961 accttcgcct tgatggtgta ggtcttgtag gccggcaggg cgacggcagc caggataccg
     7021 atgatcgcga cgacgatcat cagttcgatc agggtgaaac ccttttgaac ggtgcgcttc
     7081 atgtatgagc tccttgggag aaaagaggga cgtggctcac tcatcatgcg aatcccatgc
     7141 cagaccattc cggttgtcgg agcgcgcgca atgcgtgaat tcctgccgcg aactgacaaa
     7201 gtgcgtcacc atgagcccgc tccgccaacc attcccgtca tcggatgaca aattttgtca
     7261 gatcgagacc gctcgggcgc acgccgctca gccgcggcac gagcccggca ggtacttcgc
     7321 gtccgtgccg ctggtacgcg caggtccgca cttgaagccg ccgacctggt tgcccacgac
     7381 ggacatcgca ttgcccgccg catccgtggg cgtcagcgtg atggccgcgc tacggatctc
     7441 cgcgggcgct aggctcgtgc tgcgtggtga cggtgatcac gccgacgcta ttggtgttca
     7501 ccgagccggc gtacttactg ctcgccgttg tggtttcgca gccccaggaa ccggccgccg
     7561 gcagcgtcga agcggtctgg tagacctccg caacagcgca aggcgtcggg ctcgcgggga
     7621 gctcgaccgg gaagctgaag ccagcttgca cgccggttga cggcgagctg agcgttccga
     7681 gcgaatttcg tgccgcggcg gcgccgcttc aggcccggag aactgtcggt gtgcagacag
     7741 tttcgaaata tgaggaatgc gtcacggcgg cgcatccacg aggctcagac gtcgaagacg
     7801 tcgccccgga gcagctccat cagcgggcgc tcctgcttga gctcgcgcag gccggtcatc
     7861 acggccgcga tctcgcccgg cttgatatgg gtgatgtaga tgcgcgcgtc ggcgggtgcg
     7921 ccggccagct cggccgcgag cgagctcggg caatggtggc cgcttgcgct ggcgagcgcg
     7981 gactccgctt cgctgaacgc cgtctcgacg acgacgtgcg cgaccgggaa ttgggcgatg
     8041 cggcgccaga acgccgggtt cgggccggtg tcgccgctga atacccaggc gccgcggccg
     8101 ggcgtggccg cgtggatggc gtagccgcac gccggcaccg agtgcgccgc cggcagcacc
     8161 tcgatgcggc ggccgccgag cacgagggtg tcgccgagct cgaagggcac gaagcgcagc
     8221 accggcgccg atgcgctggg caggcgcgtg aagtcgggcc agacgaagtt gttgaacacg
     8281 tgggcgcgca gcacgtccag cgtctgcggc agcgcgtgca cctcgatcgg cgccgcgccg
     8341 cgcgcctggc ggcggcgcag cacactgtcc gccagcaacg gcacgcccag cacgtgatcg
     8401 aggtgcgagt gcgtcagcag gatgtggtcg atgcgctcga gcgcgtcgag cgacaggtcg
     8461 cccacgcccg ttccggcgtc gatgaggacg tcgtcgtcga gcaggaagct ggtggtgcgg
     8521 cagtcgcgtg cgatcgaccc ggagcagccg aggacctgga ttttcatgcg ggcatcacgc
     8581 ggttggcggc gtcgaagggt cttcgcaggc ggctggaggg ttgcccgtcc ggttcgccag
     8641 tcgggcgagc tgccgggttg cgagggcgcg caaggggggc ggcagggacc gcgcctccag
     8701 cagctcggtc agccgcgtcg tcgcgacggc gcgcgccgac ggcatcgagg ctggcggcga
     8761 gctgtcgtca tggtgccccc gcagggattc ccgtgcaagg cgccaaaggc caagttgttc
     8821 atgaaaacgg gcgatttccg ccgcgtcgcc agactccggc agcggcagcg gcacgtacag
     8881 cgtcaccagc tgggcgcggc ccttgacggc cacctcgtcg acctcgatcc aggcacagcc
     8941 gggcaactcg ccggcggccg cgcgcgtcgc gtcggcgacg agcagcgcga ggccgtagtc
     9001 gcgcgtggca ctctccaccc gcgcggcgag gttgacgccg tcgccgaccg ccgtgtagct
     9061 gcgccgcagc gccgagccga ggtcgccgac gcacacgacg ccgctcgcca gtcccatgca
     9121 cggcgcgagc gacggcagcc cgcgcgcggc gagctcggcg ttcaggggat ccacggcggc
     9181 ggccatcgcg agcgcagcgc gcaccgcatg cgccgcgtgc tgcggatcgg ccagcggcgc
     9241 gccccagaag gccatcacgg cgtcgccgat gaacttgtcg agcgtgccgc cgtgcgcgtg
     9301 gatgatctcg ctcatgcgcg agaagtacag gttcagcacc tcgcgcaggc gctcgggcgc
     9361 gagcgtctcg gacatcgtgg tgaagccgcg gaggtcgcag aacaggatcg tcagctcgcg
     9421 gttctcggca ctgctggcga cgtcgaggaa gcggtccgga tcgctggcca tcgcgcgcac
     9481 gcgctcgggc ggcaggtagt ggccgaacag gcgggcgagc gagcgccggg agtgccactc
     9541 gctcaggtag ttggcgacca ccccgccgcc gcccagcagc agggcgccgg tgagcgaggc
     9601 ggccacgggc agcgcccatc cgcgcagggc gagtgcaagc gcgtcgccgg ccaccagcgc
     9661 cgcgaggatg ccggccagcg cgagcaccgc ggccggccc
//
LOCUS       DASVLN010000063         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_486814, whole genome shotgun sequence.
ACCESSION   DASVLN010000063 DASVLN010000000
VERSION     DASVLN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486814"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1216)
                     /gene="alr"
                     /locus_tag="VF453_01385"
     CDS             complement(77..1216)
                     /gene="alr"
                     /locus_tag="VF453_01385"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7686321.1"
                     /translation="MPRPIQALIHTEALAHNVARARAAAPDARVWAVVKANAYGHGIE
                     RAFEGLRGADGFALLDFAEAERVRALGWRGPILMLEGCFEQRDLELCSRLGLWHAVHC
                     DAQIDWLAEHKTHTPHRVFLKLNSGMNRLGFTPTAFRGAWARLDKLPQVDEISLMTHF
                     SDADGPRGVSHQLAVFEEATRDLAGERTLANSAATLRHGAGPLAQAPRVGGDWVRPGV
                     MSYGCSPDYPEHTIADWDLRPTMTLRSRVIGLQALQPGDTVGYGSTFTAGDPLRIGVV
                     ACGYADGYPRHAGTGTPVLVDGVRTRIVGRVSMDMITVDLTPVPHAGLGSEVTLWGVG
                     PRPAGAPQDAPPPVLPIDEVARAAGTIGYELMCALAQRVPVAVQG"
     gene            1406..2683
                     /gene="lplT"
                     /locus_tag="VF453_01390"
     CDS             1406..2683
                     /gene="lplT"
                     /locus_tag="VF453_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEX7686322.1"
                     /translation="MKRGFYTIMAAQFFSSLADNALFAVAVDALKSTGVPAWQVTALV
                     PMFALFYVVLAPILGEFADAVPKGAVMFYSNAVKVAGCLLMLFGGHPLIAYAIVGLGA
                     AAYSPAKYGILTELLPTSQLVKANGWIEGLTILSIIAGTLLGGQLVGPRLGSVMLSIR
                     IPHVELGIHTFDQAAVVCMVVLYAVAAVFNLRIPRTGATLQSIEGNIGEIVREFSQCN
                     ARLWDDKLGQISLATTTLFWGVSANLRYIVLGWAGAALGYSMTSATAMIGVVAIGTAV
                     GAVVAAFTIRLDSAPRVILLLVGMAALVMCMDAIHSVWAAVPLLVVLGLLGGFVVVPM
                     NALLQHRGHNLMGAGRSIAVQNFNEQASILGVGAAYSLMTRLGLSVYGAIAVFGLFTA
                     GVSLLIELWHRRNLRQHPDDVQALLAIARSDSH"
     gene            2680..>3141
                     /locus_tag="VF453_01395"
     CDS             2680..>3141
                     /locus_tag="VF453_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7686323.1"
                     /translation="MSAPGARAAPVLALLVNAFVWGVSWWPFRRLQALGLHPLWATTV
                     IYVLCVAAIAAWRRNALREFATQPALWLIFLASGTTNATFNWAVSIGDVVRVVLLFYL
                     MPLWALLLARLLLGEPFTRSAIVRVALALAGAALVLSHDGSSAAGPRSPAPG"
BASE COUNT          382 a         1099 c         1159 g          501 t
ORIGIN      
        1 cgcgatgact tcggcagcaa gcccgtcacc gacgtgcgcg agtgagcggc gcgcgccccg
       61 cgcgacgccc gcgcgatcag ccctgcacgg ccaccggcac ccgctgcgcg agcgcgcaca
      121 tcagctcgta gccgatcgtg ccggccgcgc gggcgacctc gtcgatcggc agcaccggcg
      181 gcggcgcgtc ctgcggcgct ccggccgggc gcgggcccac gccccagagc gtgacctcgc
      241 tgccgaggcc ggcgtgcggg accggcgtga ggtcgacggt gatcatgtcc atcgacacgc
      301 ggccgacgat gcgcgtgcgc accccgtcca ccagcaccgg cgtgcccgtg ccggcgtggc
      361 gcgggtagcc gtccgcgtag ccgcaggcga cgacgccgat gcgcaacgga tcgcccgcgg
      421 tgaacgtgct gccgtaaccc acggtgtcgc cgggctgcag cgcctgcagg ccgatcacgc
      481 ggctgcgcag cgtcatcgtc ggccgcaggt cccagtcggc gatggtgtgc tcggggtagt
      541 cgggcgagca gccgtagctc atgacgcccg gacgcaccca atcgccgccg acgcgcggcg
      601 cctgcgcgag cggcccggcg ccgtggcgca gcgtggccgc gctgttggcg agcgtgcgtt
      661 cgcccgcgag gtcgcgcgtc gcctcctcga acaccgccag ctggtgcgac acgccgcgcg
      721 ggccgtcggc gtcggagaag tgggtcatca gcgagatctc gtcgacctgg ggcagcttgt
      781 ccaggcgggc ccaggccccg cggaacgcgg tcggcgtgaa gccgaggcgg ttcatgccgc
      841 tgttgagctt gaggaagacc ctgtgcggcg tgtgggtctt gtgctcggcg agccagtcga
      901 tctgcgcgtc gcagtgcacg gcgtgccaga gcccgaggcg cgagcacagc tcgaggtcgc
      961 gctgctcgaa gcagccctcc agcatcagga tcggtccgcg ccagcccagc gcgcgcaccc
     1021 gctcggcctc ggcgaaatcg agcagcgcga agccgtcggc gccgcgcagg ccctcgaacg
     1081 cgcgctcgat gccgtggccg taggcgttgg ccttgacgac cgcccacacg cgcgcgtccg
     1141 gtgcggcggc gcgggcgcgg gcgacgttgt gggccagggc ttcggtgtgg atcagggctt
     1201 ggatcgggcg cggcatggcg cgattctgcc agcggcggcg cgggcggtcg gggccgcccc
     1261 ggagccgggt ccgacggccg gcccgcgcgt gacttcgtgc cgaccgcccc ggatgggcgc
     1321 gtgctataaa gccgctcgcc ctaaggagag tcatttccag gcgcggggcc ggtcgggtgg
     1381 ctccgacgcc gaagacgccg aacgaatgaa acgcggtttc tacaccatca tggcggcgca
     1441 gttcttcagc tcgctcgccg acaatgcgtt gttcgccgtg gccgtggacg cgttgaagag
     1501 cacgggcgtt ccagcgtggc aggtgaccgc gctcgtgccg atgttcgccc tgttctacgt
     1561 cgtgctcgcg ccgatcctgg gcgagttcgc ggacgcggtg cccaagggcg ccgtgatgtt
     1621 ctactcgaac gccgtcaagg tggcgggttg cctcctgatg cttttcggag gccatccgct
     1681 catcgcctac gcgatcgtcg gcctgggcgc cgccgcctat tcgccggcca agtacggcat
     1741 cctgaccgag ctgctgccga cgtcgcagct ggtcaaggcc aatggctgga tcgagggctt
     1801 gaccatcctg tccatcatcg cgggcacctt gctcgggggg cagttggtcg ggccccggct
     1861 cggctcggtg atgctgtcca tccgcatccc ccacgtcgaa ctcggcatcc acacgttcga
     1921 ccaggccgcc gtggtctgca tggtggtgct ctatgcggtc gcggccgtgt tcaacctgcg
     1981 cattccgcgg acgggggcca cgctccagtc catcgaaggc aacatcggcg aaatcgtgcg
     2041 cgagttcagc caatgcaacg cgcggctgtg ggatgacaag ctgggccaga tctcgctggc
     2101 gacgacgacg ctgttctggg gcgtctcggc caacctgcga tacatcgtgc tgggttgggc
     2161 gggcgcggcc ctgggctact cgatgacctc ggcgacggcg atgatcggcg tggtcgcgat
     2221 cggcacggcg gtcggcgccg tcgtggcggc gttcaccatc cggctggact ccgcgccgcg
     2281 cgtgatcctg ctgctggtcg ggatggcggc gctcgtgatg tgcatggacg cgatccattc
     2341 ggtgtgggcg gcggtgcccc tgctggtcgt gctgggcctg ctgggcggct tcgtcgtcgt
     2401 gccgatgaac gcgctgctgc agcaccgcgg ccacaacctg atgggggccg gccgctcgat
     2461 cgcggttcag aacttcaacg agcaggcgag catcctgggc gtcggcgccg cctactcgct
     2521 gatgacgcgc ctgggcctgt cggtctacgg cgcgatcgcg gtcttcggtc tgttcacggc
     2581 cggcgtcagc ctgctgatcg agctctggca ccgtcgcaac ctgcgccagc atccggacga
     2641 cgtccaggcg ctgctcgcca tcgcccgcag cgacagccat tgagcgcgcc cggggcccgc
     2701 gcggcgccgg tgctggcgct gctggtcaac gcgttcgtct ggggcgtctc gtggtggccg
     2761 ttccggcggc tgcaggcgct cgggctgcat ccgctgtggg cgacgacggt gatctacgtg
     2821 ctgtgcgtcg ccgcgatcgc ggcctggcgg cgcaacgcgc tgcgcgaatt cgcgacgcag
     2881 cccgccctgt ggctgatctt cctcgcctcg ggcacgacca acgccacctt caactgggcc
     2941 gtcagcatcg gcgacgtggt gcgcgtcgtg ctgctgttct acctgatgcc gctgtgggcg
     3001 ctgctgctcg cgcggctgct gctgggcgag ccgttcacgc gcagcgcgat cgtgcgggtg
     3061 gcgctcgcgc tcgccggcgc ggcgctggtg ctgtcgcacg acggatcgtc cgcggccggg
     3121 ccgcgctcgc ccgcgcccgg g
//
LOCUS       DASVLN010000064         6578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_325592, whole genome shotgun sequence.
ACCESSION   DASVLN010000064 DASVLN010000000
VERSION     DASVLN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325592"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VF453_01400"
     CDS             complement(<1..1108)
                     /locus_tag="VF453_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX7686324.1"
                     /translation="MRAWPRRRVARWGVAGALGAAAVVAAALGVLCLTAGCSTVGYLS
                     QSASGHLHLLAAARPVKEVLADPALPLPLRERLELTQRIRDFATAELKEPDNGSYRSY
                     ADLHRSAAVWNVVAAPELSLKLQTWCFPIVGCVGYRGYYDLAAANEAAAPLRAQGLDV
                     DVYAVPAYSTLGWTNWIGGDPLLNTFVNWPEGEVARLIFHELAHQVAYAPGDTTFNES
                     FATAVETIGGQRWLEAHATQEVRDRQALTEQRRRDFHVLVRAWRARLDAMYRSDLPDA
                     DKRACKAEMYAAMHADYERMKRERWHGYAGYDKWFAEANNAALGAEAAYDEQVGAFIR
                     LFHAEGDDFGRFYAEVKRLARLPKAERDAGLAPYR"
     gene            complement(1108..3177)
                     /locus_tag="VF453_01405"
     CDS             complement(1108..3177)
                     /locus_tag="VF453_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX7686325.1"
                     /translation="MPTPTASPARTVHAACPHDCPDTCAIRVTVQDDKVIRLHGDPDH
                     QPTNGALCTKVSRYAERTEHAERVLRPLRRVGPKGAGRFVEVSWDEALDDIAGRLSAI
                     AARDPEAIVPYSYAGTMGMVQGEGMAARFFHKLGASRLDRTICANAGGDALAATYGGK
                     VGMHVEHFAESKLIVIWGSNSIASNLHFWPIALAAKRAGATLVAIDPRRTETAEKCHH
                     HLALLPGSDGALALSVMHELVVNDWLDHDYLARHVAGWEAMRERALQWPPERAAVECG
                     LEAQQIRDFARLYGTTRPAAIRLNYGMQRVRGGGNAVRLIALLPCLTGAWRHCAGGLL
                     LSSSGWFKAVKRAGYDHPELLAGRDPRTINMSTIGDDLLRESGEALPDGRRFGPRIEA
                     LVVYNSNPVAVAPQSGRVVAGFRREDLFTVVLEHFLTDTADHADYVLPATTQLEHWDV
                     HTSYGHTSVLVNEPALAPRGEARSNAAIFRALAARMGFDEPCLRDDDETLARQAFTGP
                     VSFDELRAHGFARLPLPEAPFADGGFPTPDGRAQCDVPAFGGVPDYVPPYESRRSAPE
                     LARRFPLAMISPPARNFLNSTFVNVKSLRDIEQEPLLEIAAEDAALRGIADGDVVRVF
                     NDRGEYRCSARVSPRARPGVVHGLGVWWRKFGRDGTNVNELTHQGLTDIGRAPSFYDC
                     LVDVAKD"
     gene            3303..4523
                     /locus_tag="VF453_01410"
     CDS             3303..4523
                     /locus_tag="VF453_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX7686326.1"
                     /translation="MNVIESLLADAAAITALRREIHSHPELCFEERRTSDLVAAKLTE
                     WGIPIRRGLARTGVVGIVEGRRGRSGGSIGLRADMDALPMSESNTFAHASRHAGRMHA
                     CGHDGHTAMLLAAAQHLAKHRDFDGTVHLLFQPAEEGGGGAREMIKEGLFDEFPMDAV
                     FGMHNWPGYGVGQFAVCPGPCMASSNEFFITIRGRGSHAALPHLAIDPVPIACQMVQA
                     FQTIVTRNKRPTDTAVISVTTIHTGEATNVVPDQAVIEGTVRTFTIPVLDLIEQRMRE
                     IAEHTAAAFGATVEFRFRRNYPPTVNHPAEAAFVREVMRETVGADNTLEFEPAMTAED
                     FSYFLLHKPGAYFVIGNGDGRHRLAYSGDEARPENLGPCTLHNPHYDFNDDLIPLGAT
                     LWINLVDRWFSRTR"
     gene            4561..5649
                     /locus_tag="VF453_01415"
     CDS             4561..5649
                     /locus_tag="VF453_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX7686327.1"
                     /translation="MSPDTDPFAPTYAQARARFRAAAQAAGARLQAHPEPGRGAGMEV
                     LAMDVARLGADDADAVLLVSSGCHGVEGHCGSGLQVALLGDAALLADAQRAGVALVLV
                     HALNPWGFSHGRRVTQEGVDLNRNFIDFRRPPPRNAGYDELADALLPADWPPPEDAER
                     RIAAYGARHGMAALQQAISGGQYAHPDGLFHGGTAPTWSHQTLRLVLRQHGARCRRLG
                     WIDLHSGLGESGVGERIHAARSDDVTRQRAKAWWGNRVTFTDDGSSVSSDLLGNAWHA
                     ACEECAQATYTGIALEFGTVPLVAVLQALRLDHWVARRHDALDPRREAARAAMRAAFY
                     TETPEWKRAVLAQGREATAQAIAGLAGR"
     gene            5746..6303
                     /locus_tag="VF453_01420"
     CDS             5746..6303
                     /locus_tag="VF453_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7686328.1"
                     /translation="MHTALIMIDVQQSFPQRPYWSQAGADAFLKHANALIDGAQARGI
                     PIVRVFHTDGPQVPTNPFAHGSEFVRPLDGLAPFEAALTIEKHRHSALVGTPLAQWLV
                     ENGIRRLIVAGIRTEQCCETTTRHASDEGWEVDFCGDATLTFDMKTPAGATLTAAEIR
                     ERTETVLVDRFCTLVDVEQALARAA"
     gene            6318..>6578
                     /locus_tag="VF453_01425"
     CDS             6318..>6578
                     /locus_tag="VF453_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7686329.1"
                     /translation="MPLPIDVFFLVLPETLLLDLAGPAEAFRLANQHALGRGQPPAFR
                     LRYVGAEPQAVTSIGMQVAGLEPLPAELPEGAWIVLMGRPGLR"
BASE COUNT          912 a         2422 c         2366 g          878 t
ORIGIN      
        1 cgcggtaggg cgcgagcccc gcgtcacgct cggccttggg cagccgcgcg agccgcttga
       61 cctcggcgta gaagcggccg aagtcgtcgc cctcggcgtg gaacaggcgg atgaacgcgc
      121 cgacctgctc gtcgtaggcg gcctcggcgc cgagcgccgc gttgttggcc tcggcgaacc
      181 acttgtcgta gcccgcgtag ccgtgccagc gctcgcgctt catgcgctcg tagtcggcgt
      241 gcatcgcggc gtacatctcg gccttgcatg cgcgcttgtc ggcatcgggc aggtcgctgc
      301 ggtacatcgc atcgagccgc gcgcgccacg cgcgcacgag gacgtggaaa tcgcgccggc
      361 gctgctcggt cagcgcctgg cggtcgcgca cctcctgcgt cgcgtgggcc tcgagccagc
      421 gctggccgcc tatcgtctcg accgcggtgg cgaaggactc gttgaaggtg gtgtcgcccg
      481 gcgcgtaggc gacctggtgc gcgagctcgt ggaagatcag ccgcgcgacc tcgccttccg
      541 gccagttgac gaaggtgttg agcagcgggt cgccgccgat ccagttcgtc cacccgagcg
      601 tggagtacgc cggcaccgcg tagacgtcga cgtccagccc ctgcgcccgc agcggcgccg
      661 ccgcctcgtt cgcggccgcg aggtcgtagt agccccggta gcccacgcag cccacgatcg
      721 ggaagcacca cgtctgcagc ttcagcgaga gctccggagc cgcgaccacg ttccacaccg
      781 cggcgctgcg gtgcaggtcg gcgtacgagc ggtagctgcc gttgtcgggc tccttcagct
      841 ccgccgtcgc gaagtcgcgg atgcgctggg tgagctcgag ccgctcgcgc agcggaagcg
      901 gcagcgcggg gtcggcgagc acctccttga ccggccgtgc cgcggccagc aggtgcaggt
      961 ggcccgacgc cgactgcgac aggtagccga ccgtcgagca gcccgccgtc aggcacagca
     1021 cgcccagcgc cgcggcgacc acggcggcgg cgccgagcgc gccggccacg ccccagcggg
     1081 ccacgcggcg tcggggccag gcgcgcatca gtccttcgcc acgtcgacga ggcagtcgta
     1141 gaacgacggc gcccggccga tgtccgtcag gccctggtgc gtgagctcgt tgacgttggt
     1201 gccgtcgcgg ccgaacttgc gccaccacac gcccaggccg tgcacgacgc cggggcgcgc
     1261 gcgcgggctc acgcgggcgc tgcaacggta ctcgccgcgg tcgttgaaga cccgcaccac
     1321 gtcgccgtcg gcgatgccgc gcagggcggc gtcctcggcg gcgatctcca gcagcggctc
     1381 ctgctcgatg tcgcgcaggc tcttgacgtt gacgaaggtg gagttgagga agttgcgcgc
     1441 cggcggcgag atcatcgcga gcgggaagcg gcgcgcgagc tcgggcgcgc tgcgcctgct
     1501 ctcgtagggc ggcacgtagt ccggcacgcc gccgaacgcc ggcacgtcgc actgcgcgcg
     1561 gccgtcgggc gtcgggaagc cgccgtcggc gaacggcgcc tcgggcaggg gcaggcgcgc
     1621 gaagccgtgc gcgcgcagct cgtcgaagga caccggcccg gtgaaggcct ggcgcgcgag
     1681 cgtctcgtcg tcgtcgcgca ggcagggctc gtcgaaaccc atgcgcgcgg ccagcgcgcg
     1741 gaagatcgcc gcgttcgagc gcgcctcgcc ccgcggcgcg agcgccggct cgttgaccag
     1801 caccgaggtg tggccgtagc tggtgtggac gtcccagtgc tccagctgcg tggtcgcggg
     1861 cagcacgtag tcggcgtggt cggcggtgtc ggtgaggaag tgctccagca cgacggtgaa
     1921 caggtcctcg cgccggaagc cggcgaccac gcgcccggac tgcggcgcca ccgccaccgg
     1981 gttgctgttg tagacgacca gcgcctcgat gcgcggcccg aagcgccggc cgtccggcag
     2041 cgcctcgccg ctctcgcgca gcaggtcgtc gccgatcgtg ctcatgttga tcgtgcgcgg
     2101 gtcgcggccg gcgagcagct cgggatggtc gtagccggcg cgcttgaccg ccttgaacca
     2161 gcccgaggac gagagcagca ggccgcccgc gcagtggcgc cacgcgccgg tcaggcaggg
     2221 cagcagcgcg atcaggcgca cggcgttgcc gccgccgcgc acgcgctgca tgccgtagtt
     2281 caggcggatc gcggccggcc tggtggtgcc gtacaggcgg gcgaagtcac ggatctgctg
     2341 cgcctccagg ccgcattcga ccgccgcgcg ctcgggcggc cactgcagcg cgcgctcgcg
     2401 catcgcctcc cagccggcga cgtggcgggc gaggtagtcg tggtcgagcc agtcgttgac
     2461 gacgagctcg tgcatcaccg acagcgcgag cgcaccgtcg ctgccgggca gcagcgcgag
     2521 gtggtggtgg catttctcgg ccgtctcggt gcggcgcggg tcgatcgcca ccagcgtcgc
     2581 gccggcgcgc ttggcggcca gggcgatcgg ccagaagtgc aggttcgacg cgatcgagtt
     2641 gctgccccag atcacgatca gcttcgattc ggcgaagtgc tcgacgtgca tgcccacctt
     2701 gccgccgtag gtcgcggcga gggcgtcgcc gccggcgttg gcgcagatcg tgcgatccag
     2761 gcgcgaggcg cccagcttgt ggaagaagcg cgccgccatg ccctcgccct gcaccatgcc
     2821 catggtgccg gcatagctgt agggaacgat cgcctcggga tcgcgcgccg cgatcgccga
     2881 caggcggccg gcgatgtcgt ccagcgcctc gtcccaggac acctcgacga accgaccggc
     2941 gcccttgggg ccgacgcgcc tgagcggcct gagcacgcgc tcggcatgct ccgtgcgctc
     3001 ggcgtagcgc gagaccttgg tgcacagcgc gccgttggtc ggctggtggt cgggatcgcc
     3061 gtgcaggcgg atcaccttgt cgtcctgcac cgtgacgcgg atggcgcagg tgtccgggca
     3121 atcgtgcggg caggccgcgt gcacggtgcg cgcgggggag gcggtcgggg tcggcacgtc
     3181 gatcattcca ggatgatgtc acagcgccca tgccgcaccg ggccgcacgg gcacgcgggg
     3241 cggcactgtt tcacgggccc cggcgggact aagatggctc tttgcgcccg ccggttcccg
     3301 ccatgaacgt catcgaatcc ctcctggccg acgccgctgc gatcaccgcg ctgcgccgcg
     3361 agatccactc ccatcccgag ctctgcttcg aggaacggcg caccagcgac ctcgtggccg
     3421 ccaagctgac cgagtggggc atccccatcc ggcgcgggct ggcgcggacg ggggtagtcg
     3481 gcatcgtcga gggccggcgc ggccgctcgg gcgggtcgat cgggctgcgc gccgacatgg
     3541 acgccctgcc catgtccgag tccaacacct tcgcccacgc cagccggcac gccggccgca
     3601 tgcacgcctg cggacacgac ggccacaccg cgatgctgct ggccgcggcg cagcacctcg
     3661 cgaagcaccg cgacttcgac ggcacggtcc acctgctgtt ccagcccgcc gaggaaggcg
     3721 gcggcggcgc gcgcgagatg atcaaggaag gactgttcga cgagttcccg atggacgccg
     3781 tcttcggcat gcacaactgg ccgggctacg gtgtcggcca gttcgcggtc tgcccgggcc
     3841 cgtgcatggc ctcgagcaac gagttcttca tcacgatccg cggccgcggc tcgcatgcgg
     3901 ccctgccgca cctggcgatc gacccggtgc cgatcgcctg ccagatggtg caggcgttcc
     3961 agaccatcgt cacgcgcaac aagcggccga cggacacggc cgtgatctcg gtgacgacca
     4021 tccacaccgg cgaggccacc aacgtcgtgc ccgaccaggc ggtgatcgaa ggcacggtgc
     4081 gcaccttcac gatcccggtg ctcgacctga tcgagcagcg catgcgcgag atcgccgagc
     4141 acacggcggc cgcgttcgga gcgacggtcg agttccgctt ccgccgcaac tacccgccca
     4201 ccgtcaacca tccggccgag gccgccttcg tgcgcgaggt gatgcgcgag acggtcggcg
     4261 ccgacaacac gctcgagttc gagcctgcga tgacggccga ggacttcagc tacttcctgc
     4321 tgcacaagcc gggcgcgtac ttcgtgatcg gcaacggcga cggtcgccac cggctcgcct
     4381 acagcggcga cgaggcgcgt cccgagaacc tgggcccctg cacgctgcac aacccgcact
     4441 acgacttcaa cgacgacctg atcccgctcg gcgcgacgct gtggatcaac ctggtcgacc
     4501 gctggttctc gcgcacgcgc tgacgcccgc gcgcggcgcg cgccaggagg aggagccccg
     4561 atgagcccgg acaccgatcc gttcgcgccg acctacgccc aggcgcgcgc gcgattccgt
     4621 gccgccgcgc aggccgccgg cgcgaggttg caggcgcatc ccgaaccggg gcgcggcgcc
     4681 ggcatggagg tgctggcgat ggacgtcgca cgcctgggcg ccgatgacgc ggacgcggtg
     4741 ctgctcgtct ccagcggctg ccacggggtg gagggccact gcggctccgg cctgcaagtg
     4801 gcgctgctgg gcgacgccgc gctgctggcg gatgcgcagc gcgcaggcgt ggcgctcgtg
     4861 ctcgtgcacg cgctcaatcc gtggggcttc tcgcatggcc ggcgcgtgac gcaggagggc
     4921 gtcgacctca accgcaactt catcgacttc cgccgcccgc ccccgcgcaa cgccggctac
     4981 gacgagctcg ccgacgcgct gctgccggcc gactggccac cgcccgaaga tgccgagcgc
     5041 cgcatcgcgg cctacggcgc acgccacggg atggcggcac tgcagcaggc gatctcgggc
     5101 ggccagtacg cccatccgga cgggctgttc cacggcggca ccgcgccgac ctggagccac
     5161 cagacgctgc gcctggtgct gcgccagcac ggcgcgcgct gccgccggct cggctggatc
     5221 gacctccaca gcggcctggg cgagagcggc gtgggcgagc gcatccacgc cgcacgctcc
     5281 gacgacgtca cgcgccagcg cgcgaaggcc tggtggggca accgcgtcac gttcacggac
     5341 gacggctcgt ccgtctcctc cgacctgctg ggcaacgcct ggcacgcggc ctgcgaggaa
     5401 tgcgcgcagg cgacctacac cggcatcgcg ctcgagttcg gcaccgtgcc gctggtcgcg
     5461 gtgctgcagg cgctgcgcct ggaccactgg gtcgcgcgcc ggcatgacgc gctcgacccg
     5521 aggcgcgagg cggcgcgcgc cgcgatgcgc gcggcgttct acaccgagac gccggaatgg
     5581 aagcgcgccg tgctcgccca gggccgcgag gcgaccgccc aggccatcgc cggcctggcc
     5641 ggccgctgac gatgtcctaa actgtccgca agcggtcgcg cccggccgct gcgcgaggcg
     5701 tgcgcggccg gcaagatgac gtccatccca accacgagaa gcgccatgca caccgccctc
     5761 atcatgatcg acgtccagca atcgttcccg cagcgcccgt actggtcgca agccggcgcc
     5821 gacgcgtttc tgaagcacgc caacgcgctc atcgacggcg cgcaggcgcg cggcatcccc
     5881 atcgtgcgcg tgttccacac cgacggcccg caggtgccga ccaacccgtt cgcccacggc
     5941 agcgagttcg tgcgccccct cgacggcctc gcgcccttcg aggcggcgct gacgatcgag
     6001 aagcatcgcc acagcgcgct cgtgggcacg ccgctcgcgc agtggctcgt cgagaacggc
     6061 atccgccggc tgatcgtcgc gggcatccgc accgagcagt gctgcgagac cacgacacgc
     6121 cacgcgagcg acgaaggctg ggaggtcgac ttctgcggcg acgcgacgct caccttcgac
     6181 atgaagaccc ccgcgggcgc gacgctcacg gccgccgaga tccgcgagcg caccgagacc
     6241 gtcctcgtcg accgcttctg cacgctggtc gacgtcgaac aggcgctcgc ccgcgcagcc
     6301 tgaccacccc cttgtccatg cccctgccga tcgacgtctt cttcctcgtg ctgcccgaga
     6361 cgctgctgct ggacctcgca ggcccggccg aggcgttccg gctcgccaac cagcatgcgc
     6421 tggggcgcgg gcagccgccc gcgttccggc tgcgctacgt cggtgccgag ccgcaggcgg
     6481 tgacctcgat cggcatgcag gtcgcgggac tggagccgct gccggccgag ctgccggagg
     6541 gcgcatggat cgtgctgatg ggacgccccg gcctgcgc
//
LOCUS       DASVLN010000065         8812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_164816, whole genome shotgun sequence.
ACCESSION   DASVLN010000065 DASVLN010000000
VERSION     DASVLN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164816"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2150)
                     /gene="pheT"
                     /locus_tag="VF453_01430"
     CDS             complement(<1..2150)
                     /gene="pheT"
                     /locus_tag="VF453_01430"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7686330.1"
                     /translation="MQFPESWLREFCNPPITTNELAELLTMAGLEVEEMRPVAPPFGG
                     IVVAEIVEAVQHPNADKLRVCKVDAGAHSPDGPLQIVCGAPNARAGIRVPLAMVGAEL
                     PPGDDGKPFRIGVGKLRGVESFGMLCSARELKLSEDHAGLLELPGDTPLGIDVREALT
                     LDDTLFTLKLTPNLAHNLSVLGIAREVSALTGAPLKTPSFAPVAVTHQDNVPVRIEAP
                     DLCGRWVGRVVHGVDPKATTPAWIVERLARCGQRSVSPLVDISNYVMFELGRPSHIFD
                     HAKIHDELVIRWAKPGETLKLLNGNTVTLDEKIGVVADSAGPESLAGIMGGEASAVSD
                     STTSVYVEAAFWWPQAVAGRSRRFNFSTDAGHRFERGVDAETIAVHVERITQLILDVC
                     GGAAGPVNDVVSRLPERRPVAMRVARAAKVIGMPVAQAECAGVMQRLGLQFVEGEGTI
                     TVTPPSWRFDLQIEEDLIEEVVRVLGFGRLPDTPPVAPVTARIRPEASRSTHAVRRAI
                     AALDYQETINFSFVEARWEHELAGNPTPIQVLNPIAAPLAVMRSSLVGSLVQVLRHNL
                     ARKAPRVRVFELGRVFRRAPQVASTLATVAGIDQPMRVGGLAWGPADTLQWGVTERHV
                     DFFDVKGDVEALLAPRAVSFVADEHPALHPGRCAKVLVDGQAIGHVGELHPRWRQSYD
                     LPSAPVVFELDLAAVQARPVPVAQPLPRQQAARR"
     gene            complement(2208..3233)
                     /gene="pheS"
                     /locus_tag="VF453_01435"
     CDS             complement(2208..3233)
                     /gene="pheS"
                     /locus_tag="VF453_01435"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7686331.1"
                     /translation="MNDLDLLVQSAEADFSAAATPADLENAKARFLGKSGRVTELLKG
                     LAALPPEDKKTRGAEINVVKQRIEAALAARRQALADAELQAQLKAEALDVTLPGRARG
                     TGGLHPVTRTLERIEQIFGSMGFDVADGPEIEEDWYNFTALNTPEDHPARSMHDTFYV
                     EGGYVLRTHTSPMQVRYAMQHVQRHKESARMPEIRVIAPGRTYRVDSDATHSPMFHQC
                     EGLWIGENVSFKDLRAVFTDFLRQFFETDDLEIRFRPSFFPFTEPSAEVDIAFASGPL
                     KGRWLEVAGSGQVHPNVVRNFGLDPERYIGFAFGMGPDRLTMLRYGVNDLRQFFDGDL
                     RFLSQFR"
     gene            complement(3393..3749)
                     /gene="rplT"
                     /locus_tag="VF453_01440"
     CDS             complement(3393..3749)
                     /gene="rplT"
                     /locus_tag="VF453_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX7686332.1"
                     /translation="MPRVKRGVTARARHKKILALAKGFRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRTRKRVFRALWIARINAAARSHGVTYSRFIAGLKKAAIDLDRKVLADLAVND
                     PAAFTSIVDKVKASLA"
     gene            complement(3778..3981)
                     /gene="rpmI"
                     /locus_tag="VF453_01445"
     CDS             complement(3778..3981)
                     /gene="rpmI"
                     /locus_tag="VF453_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX7686333.1"
                     /translation="MPKMKTKKSAAKRFRVRPGGTVKRGQAFKRHILTKKTTKNKRHL
                     RGAVNVHETNMGHMAQMLPFAGL"
     gene            complement(4177..4704)
                     /gene="infC"
                     /locus_tag="VF453_01450"
     CDS             complement(4177..4704)
                     /gene="infC"
                     /locus_tag="VF453_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX7686334.1"
                     /translation="MPNVERKHRLNREITAPMVRLNGPENEPLGVVSLQEALRLSGEY
                     DVDLVEIAAQADPPVCRLMDYGKFKYLEQKKAADAKSKQKVIEVKEVKFRPGTDEGDY
                     NIKMRNLRRFIAEDGDKGKVTLRFRGREITHQEIGMRLLERIRDELADVSVVEHMPKL
                     EGRQMIMVLAPKRKS"
     gene            complement(4855..6774)
                     /gene="thrS"
                     /locus_tag="VF453_01455"
     CDS             complement(4855..6774)
                     /gene="thrS"
                     /locus_tag="VF453_01455"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7686335.1"
                     /translation="MIAIQLPDGSKREFAQPVTVADVAASIGAGLAKAALGGRVGKPG
                     HTKLVDTSYLIEQDESLAIVTDKDPDGLDMIRHSTAHLLAYAVKELFPEAQVTIGPVI
                     ENGFYYDFSYKRPFTPEDLEAIEKKMGELAKKDEKVTRRVLPRDEAVAYFKGIGEAYK
                     AEIIGSIPAGEDVSLYREGAFEDLCRGPHVPSTGKLKHFKLMKVAGAYWRGDHRNEML
                     QRVYGTAWATKEELQQYLFMLEEAEKRDHRKLGRELDLFHIDDHAPGMVFWHPKGWAV
                     WQGVEQYMRQVYRDNGYQEVKGPQVIDKSLWELSGHWDKYKDAMFTTESEKREYALKP
                     MNCPGHILIFKQGIKSYRDLPLRFGEFGNCHRNEPSGGLHGIMRVRGFTQDDGHIFCT
                     EDQILPECLAYTALLQKVYKDFGFTDIIYKVATRPAARIGSDEAWDKAENALIKSLEA
                     TGVEVVIAPGDGAFYGPKIEYTLKDALGRQWQCGTMQVDFFMAQRLGAEYVAESGERQ
                     TPVMLHRAIVGSLERFIGILIEQHAGALPAWLAPVQVVVACITEGQADYVRDVVNSLR
                     QQGIRAEADLRNEKITYKIREHSLQKVPFILVVGDKEKASGAVAVRARGNRDLGVMPL
                     ADFQRKLAEDIAAKV"
     gene            complement(7009..8097)
                     /locus_tag="VF453_01460"
     CDS             complement(7009..8097)
                     /locus_tag="VF453_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7686336.1"
                     /translation="MAALQVFAGPAARARLRERGLQPGDVRTIPGAAGGPKGLVLNGL
                     DRFLFGHWLPRGAQPVHLLGASIGAWRLATACLPDPDAALATMARDYIDQVYEHEPGK
                     PPKARHVSELFAAKLAERFGGNEARVLAHPRLRLHVFTSRGVRALARDGRLRTPLGYL
                     AAFAANAVSRRAMGSLLERVVFSDPRDPMPVPVHDYRTRHVALDAANLRESILASCSI
                     PFWLEAVQGIAGAPPGPYWDGGITDYHLHLDYAAADAPAGDEPALVLYPHFQPTLVPG
                     WLDKPLRRRHRASARLSNVVVLAPSPEWVATLPAGKIPDRGDFKAHGDDHSSRMAAWR
                     RATAEGARLAEEFDILSRRTSIEALPLP"
     gene            complement(8144..8737)
                     /locus_tag="VF453_01465"
     CDS             complement(8144..8737)
                     /locus_tag="VF453_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX7686337.1"
                     /translation="MRRFASPLVLVPTLVVSAGLLSACAHKPATPAAATAEPAKVTES
                     IFSRTRSAVATLSPAAGGTATGIVTFSASGNAVDVHVQASGLVPGSVHGIHVHETGNC
                     ASPDFMSAGGHFNPTHQPHGPQTGPHHLGDMPSLLADPSGKVDAKFTLNGVALGGTDG
                     YVGRAVILHAGPDDYATQPTGNSGGRIACGVIATTEQ"
BASE COUNT         1247 a         2978 c         3185 g         1402 t
ORIGIN      
        1 tcgcgccgcg ccgcctgctg gcgcggcagc ggctgcgcga cgggcaccgg ccgcgcctgc
       61 acggccgcga ggtcgagctc gaacaccacc ggcgccgacg ggaggtcgta cgactggcgc
      121 cagcgcggat gcagctcgcc cacgtggccg atcgcctggc cgtcgacgag caccttcgcg
      181 caacggcccg gatgcagcgc cggatgctcg tcggcgacga agctcaccgc gcgcggcgcc
      241 agcagcgcct cgacgtcgcc cttgacgtcg aagaagtcca cgtggcgctc ggtcacgccc
      301 cactgcagcg tgtccgccgg gccccacgcc agcccgccca cgcgcatcgg ctggtcgatg
      361 ccggccaccg tcgcgagcgt cgacgcgacc tgcggtgcac ggcggaacac gcggcccagc
      421 tcgaacaccc gcacgcgcgg cgccttgcgc gcgaggttgt ggcgcagcac ctgcacgagg
      481 ctgccgacca ggctggagcg catcaccgcg agcggcgcgg cgatcgggtt gagcacctgg
      541 atcggggtcg ggttgcccgc gagctcgtgc tcccagcgcg cctcgacgaa gctgaagttg
      601 atcgtctcct ggtagtcgag cgcggcgatc gcgcggcgca ccgcatgggt cgagcggctc
      661 gcctcggggc ggatgcgggc ggtcaccggc gcgaccggcg gcgtgtcggg caggcggccg
      721 aagccgagca cccgcacgac ctcctcgatc aggtcctcct cgatctgcag gtcgaagcgc
      781 cagctcggcg gcgtcacggt gatcgtgccc tcgccttcga cgaactgcag gcccaggcgc
      841 tgcatcacgc ccgcgcactc cgcctgcgcg accggcatgc cgatcacctt ggcggcgcgc
      901 gcgacgcgca tcgccacggg ccggcgctcg ggcagccgcg agaccacgtc gttgacaggg
      961 cccgcagcgc cgccgcacac gtcgaggatc agctgcgtga tccgctcgac gtgcacggcg
     1021 atggtctcgg catcgacccc gcgctcgaag cggtgccccg cgtcggtcga gaagttgaag
     1081 cgccgcgagc gtcccgcgac ggcctgcggc caccagaacg cggcctcgac gtagacgctg
     1141 gtggtcgagt cggagacggc cgaggcctcg ccgcccatga tgccggcgag cgactcgggg
     1201 cctgcgctgt cggcgacgac gccgatcttc tcgtcgagcg tcaccgtgtt gccgttcagc
     1261 agcttgagcg tctcgcccgg cttcgcccag cggatcacca gctcgtcatg gatcttcgcg
     1321 tggtcgaaga tgtgcgacgg gcggccgagc tcgaacatca cgtagttgga gatgtcgacc
     1381 agcggcgaga cggagcgctg gccgcagcgt gccaggcgct cgacgatcca cgccggcgtc
     1441 gtcgccttcg ggtccacgcc gtgcacgacg cggcccaccc agcggccgca caggtcgggc
     1501 gcctcgatgc gcaccggcac gttgtcctgg tgcgtgaccg cgacgggcgc gaacgacggc
     1561 gtcttcagcg gcgcgccggt cagcgccgac acctcgcgcg cgatgcccag cacggacagg
     1621 ttgtgcgcga ggttgggcgt cagcttgagc gtgaacagcg tgtcgtcgag cgtcagcgcc
     1681 tcgcggacgt cgatacccaa gggtgtatcg cccggcagct cgagcaggcc ggcatggtcc
     1741 tcggacagct tgagctcgcg cgccgagcac agcatgccga agctctcgac gccgcgcagc
     1801 ttgcccacgc cgatcctgaa cggcttgccg tcgtcgccgg ggggcagctc ggcacccacc
     1861 atcgccagcg gcacgcggat gcccgcgcgc gcgttcggcg cgccgcagac gatctgcagc
     1921 gggccgtccg gcgagtgcgc gccggcgtcg accttgcaca cgcgcagctt gtcggcgttg
     1981 ggatgctgca cggcctcgac gatctcggcg acgacgatgc cgccgaacgg cggcgccacg
     2041 ggacgcatct cctcgacctc gaggccggcc atggtcagca gctcggcgag ctcgttcgtc
     2101 gtgatcggcg gattgcagaa ttcgcgcagc caggattccg ggaattgcat ggtcggggct
     2161 cgggggagaa tcgttcgggc gggagtcgtg cggcgggcgg cctggcgtca gcggaactgc
     2221 gacaggaagc gcaggtcgcc atcgaagaac tggcgcaggt cgttgacgcc gtagcgcagc
     2281 atggtcaggc ggtccggccc catgccgaag gcgaagccga tgtagcgttc cgggtcgagg
     2341 ccgaagttgc gcacgacgtt cgggtgcacc tggccggaac cggcgacctc gagccagcgc
     2401 cccttcagcg gcccggacgc gaacgcgatg tcgacctcgg ccgagggctc cgtgaacggg
     2461 aagaacgacg ggcggaagcg gatctcgagg tcgtcggtct cgaagaactg gcgcaggaag
     2521 tccgtgaaga cggcgcgcag gtccttgaag ctcacgttct cgccgatcca caggccctcg
     2581 cactggtgga acatcggcga gtgcgtcgcg tcgctgtcga cgcggtaggt gcggcccggc
     2641 gcgatcacgc ggatctcggg catgcgcgcc gactccttgt ggcgctggac gtgctgcatc
     2701 gcgtagcgca cctgcatcgg gctcgtgtgc gtgcgcagca cgtagccgcc ttcgacgtag
     2761 aaggtgtcgt gcatcgaacg cgccggatgg tcctcgggcg tgttgagggc ggtgaagttg
     2821 taccagtcct cctcgatctc ggggccgtcg gcgacgtcga agcccatcga gccgaagatc
     2881 tgctcgatgc gctcgagcgt gcgcgtgacc ggatgcaggc ccccggtgcc gcgcgcgcgg
     2941 cccggcagcg tcacgtccag cgcctccgcc ttcagctgcg cctgcagctc ggcgtcggcg
     3001 agcgcctggc ggcgcgccgc caacgcggcc tcgatgcgct gcttgacgac gttgatctcg
     3061 gcgccgcggg tcttcttgtc ctcgggcggg agcgcggcca ggcccttgag cagctcggtc
     3121 acccggcccg acttgccgag gaagcgcgcc ttcgcgttct ccaggtcggc cggcgtggcg
     3181 gcggcggaga agtcggcttc ggcggactgg accaggagat cgaggtcgtt catggcgaat
     3241 tcgtgaggcg ggaaacgagg aggcgttcgg tgcggaaaaa gaaaaggccg gcacccaggg
     3301 tgtcggcctt gtcggcgaga cgcggcgcgc ggccgcattc tcgcaggctg gtccgcggcc
     3361 ggtccctcag gcgccggcgc gggggatcac gttcaagcca gcgaagcctt caccttgtcg
     3421 acgatgctcg taaaggcggc gggatcgttg acggcgaggt cggccaggac cttgcggtcg
     3481 aggtcgatcg cggccttctt caggccggcg atgaagcggc tgtacgtcac gccgtgcgaa
     3541 cgcgcggcgg cgttgatacg ggcgatccac agggcgcgga acacgcgctt gcgggtacgg
     3601 cggtcgcggt aggcgtactg gcccgccttc atcaccgcct gcttggcgac gcgatagacg
     3661 ttcttgcggc ggccgcggaa gcccttggcg agggcgagga tcttcttgtg gcgggcgcgg
     3721 gcggttacac cacgtttgac gcgaggcatg tcggtttcct ttcagtccgt cgaagaatca
     3781 gagaccggcg aacggcagca tctgcgccat gtggcccatg ttggtctcgt gcacgttcac
     3841 cgcaccacgc aggtggcgct tgttcttcgt ggtcttcttc gtcaggatgt ggcgcttgaa
     3901 ggcctggccg cgcttgacgg taccacccgg acggacgcgg aaccgcttgg ccgcgctctt
     3961 cttggtcttc atcttgggca tggaatgctc ctgttgatgc accccggacc aggcgagcgg
     4021 cgaacggatg ccactgcttg tcggcctgcc ggagcttgtt ggcgtcccgc cgtgaaaacg
     4081 ccccggcagc tgcgctgacg aggcgtcctt cgaagggaat cgccggcttt ccgccgccgc
     4141 gcggggcgcg gatcggcgaa aacccgtgat tatagctcag ctcttgcgct tgggagcaag
     4201 caccatgatc atttgccggc cttccagctt gggcatgtgc tcgaccacgg agacgtcggc
     4261 cagctcgtcg cggatgcgct cgagcaggcg catgccgatc tcctggtgcg tgatctcgcg
     4321 accgcggaag cgcagcgtca ccttgccctt gtcgccgtcc tcggcgatga agcggcgcag
     4381 gttgcgcatc ttgatgttgt agtcgccctc gtccgtgccc ggacggaact tcacttcctt
     4441 gacctcgatg accttctgct tggacttcgc gtccgcggcc ttcttctgct cgaggtactt
     4501 gaacttgccg tagtccatca gccggcagac cggcgggtcc gcctgggccg cgatctcgac
     4561 caggtccacg tcgtactcgc cggacaggcg cagcgcctcc tggagactca cgacgccgag
     4621 cggttcgttc tcaggtccgt tcagccgcac catgggcgcg gtgatttcac gattgagccg
     4681 gtgcttgcgc tcgacattgg gcatccgacg gtcagcgaag gtagcgatgg ttcgattcct
     4741 aaatgcgaat cccgggcaag agcgggacgc ttgaagcaag gggtggtgga cgttccggct
     4801 ggaccgccgg ccggcgcggg aggcaattgc gactcccgct ccggctcggt ttcgtcagac
     4861 cttggcagcg atgtcctcgg cgagcttccg ctggaagtct gcaagaggca tcacgccgag
     4921 atcccggttg ccccgggcgc gcaccgcgac ggccccgctt gccttttcct tgtcgccgac
     4981 gacgaggatg aacggaacct tctgcaacga atgctcccgg attttatagg tgatcttttc
     5041 gttgcgcaaa tcggcctcgg cccgaatccc ctgctggcgg agcgagttca ccacgtcgcg
     5101 gacatagtcg gcctgcccct ccgtgatgca ggcgaccacc acctggacgg gcgcgagcca
     5161 ggccggcagc gcgccggcgt gctgctcgat caggatgccg atgaagcgct ccaggctgcc
     5221 gacgatcgcg cggtggagca tgaccggcgt ctggcgctcg ccgctttccg ccacgtactc
     5281 ggccccgagg cgctgcgcca tgaagaagtc gacctgcatc gtgccgcact gccactgccg
     5341 gccgagcgcg tccttgagcg tgtactcgat cttcggcccg tagaacgcgc cgtcgcccgg
     5401 ggcgatcacg acctcgacgc cggtcgcctc caggctcttg atgagcgcgt tctcggcctt
     5461 gtcccaggcc tcgtccgagc cgatgcgcgc ggccgggcgc gtcgcgacct tgtagatgat
     5521 gtcggtgaag ccgaagtcct tgtagacctt ctgcagcagc gccgtgtagg ccaggcattc
     5581 gggcaggatc tggtcttccg tgcagaagat gtggccgtcg tcctgcgtga agccgcgcac
     5641 gcgcatgatg ccgtgcaggc cgccgctggg ctcgttgcga tggcagttgc cgaactcgcc
     5701 gaagcgcagc ggcaggtcgc ggtagctctt gatgccctgc ttgaagatca ggatgtggcc
     5761 cgggcagttc atcggcttga gcgcgtattc ccgcttctcg ctctcggtcg tgaacatcgc
     5821 gtccttgtac ttgtcccagt ggccgctgag ctcccacagg gacttgtcga tcacctgcgg
     5881 gcccttgacc tcctggtagc cgttgtcgcg gtacacctgg cgcatgtact gctcgacgcc
     5941 ctgccacacg gcccagccct tcggatgcca gaacaccatg cccggcgcgt ggtcgtcgat
     6001 gtggaacagg tcgagctcgc ggccgagctt gcggtggtcg cgcttctcgg cctcctcgag
     6061 catgaacagg tactgctgca gctcctcctt ggtcgcccag gccgttccgt agacgcgctg
     6121 cagcatctcg ttgcggtggt cgccccgcca gtaggcgccg gccaccttca tgagcttgaa
     6181 gtgcttgagc ttgcccgtgc tcggcacgtg cgggccgcgg cagaggtcct cgaaggcgcc
     6241 ctcgcggtag agcgagacgt cctcgcccgc cgggatggag ccgatgatct cggccttgta
     6301 cgcctcgccg atgcccttga agtaggcgac ggcctcgtcg cgcggcagca cgcgccgggt
     6361 gaccttctcg tccttcttcg cgagctcgcc catcttcttc tcgatcgcct cgaggtcctc
     6421 gggcgtgaag gggcgcttgt acgagaagtc gtagtagaag ccgttctcga tgaccggccc
     6481 gatcgtcact tgggcctcgg ggaacagctc cttgaccgcg tacgcaagca ggtgcgcggt
     6541 cgagtggcgg atcatgtcca gcccgtcggg atccttgtcc gtgacgatgg cgagcgactc
     6601 gtcctgctcg atgaggtagc tggtgtcgac cagcttcgtg tggccaggct tgccgacacg
     6661 ccccccgagc gcggccttgg cgaggccggc gccgatcgag gcggcgacgt ctgcgacggt
     6721 caccggctgc gcgaactcgc gcttggaacc gtcgggcagt tggatagcga tcatcgggaa
     6781 agtctccggg caacaaaaaa gcgcggacct cacggccgcg cttcttcagg gaaagggaaa
     6841 agtcgtcttc gacgcgtcag cacgccacgg ccgggcgcac tccgcgggga gtgtcgagcg
     6901 agagcgtagt tcgcggtgtc ataacccgtt gcgcctttct cgttcttgcc gtgtggtgga
     6961 tggaggggtg gatccagcgg attctagcgg acgcgcgcgc cgccgccttc aggggagagg
     7021 aagcgcctcg atcgaggtcc ggcgcgacag gatgtcgaac tcctccgcca gccgcgcgcc
     7081 ctccgcggtg gcgcggcgcc aggcggccat gcgcgaggag tggtcgtcgc catgggcctt
     7141 gaagtcgccg cggtcgggga tcttgccggc cggcagggtc gcgacccact cgggcgacgg
     7201 cgcgagcacc acgacgttgg acaggcgcgc gctcgcgcgg tggcggcggc gcagcggctt
     7261 gtcgagccag ccgggcacca gcgtcggctg gaagtgcggg tacagcacga gcgccggctc
     7321 gtcgcccgcg ggcgcgtccg cggcggcgta gtccaggtgc aggtggtagt cggtgatgcc
     7381 gccgtcccag tacgggccgg gcggcgcgcc cgcgatgccc tgcacggcct cgagccagaa
     7441 cgggatcgag cagctcgcga ggatgctctc gcgcaggttg gccgcgtcga gcgcgacgtg
     7501 gcgcgtgcgg tagtcgtgca ccggcacggg catcgggtcg cgcgggtcgg agaagacgac
     7561 gcgctccagc aggctgccca tcgcgcgccg cgagacggcg ttggccgcga aggccgcgag
     7621 gtagcccagc ggcgtccgca ggcggccgtc gcgtgcgagc gcccgcacgc cgcgactcgt
     7681 gaagacgtgc agccgcaggc gcggatgcgc gagcacccgc gcctcgttgc cgccgaagcg
     7741 ctcggccagc ttggcggcga agagctcgct gacgtggcgc gccttgggcg gcttgccggg
     7801 ctcgtgctcg tagacctggt cgatgtagtc gcgcgccatc gtcgcgagcg cggcgtccgg
     7861 atcgggcagg caggcggtgg ccaggcgcca ggcgccgatc gaggcgccca gcaggtgcac
     7921 cggctgcgcg ccgcgcggca accagtggcc gaacaggaag cggtcgagcc cgttgagcac
     7981 gagccccttg gggccgcccg ccgcgcccgg gatcgtgcgc acgtcgcccg gctgcagccc
     8041 gcgttcgcgc aggcgcgcgc gcgccgccgg cccggcgaag acctgcagcg ccgccatcag
     8101 ccgcgcacgg ccggcgacgc gacgacgttc gcggacgccg cgctcactgc tcggtcgtcg
     8161 cgatcacgcc gcacgcgatg cggccgccgg agttgccggt cggctgcgtc gcgtagtcgt
     8221 cggggcccgc gtgcaggatg acggcgcggc cgacgtagcc gtcggtgccg cccagcgcga
     8281 cgccgttgag cgtgaacttg gcgtcgacct tgccgctcgg gtcggcgagc aggctgggca
     8341 tgtcgcccag gtggtggggg ccggtctgcg ggccgtgcgg ctggtgggtc ggattgaaat
     8401 ggccgccggc gctcatgaag tcggggctgg cgcagttgcc ggtctcgtgg acgtggatgc
     8461 cgtgcaccga gcccggcacg aggccgctcg cctgcacgtg cacgtcgacc gcgttgccgc
     8521 tggccgagaa ggtcacgatg ccggtggccg tgccgccggc cgcgggcgag agcgtcgcga
     8581 ccgcgctgcg cgtgcggctg aagatgctct cggtgacctt ggccggctcc gccgtcgcgg
     8641 ccgccggggt cgccggcttg tgggcgcagg ccgagagcag gcccgccgag acgacgagcg
     8701 tcggaacgag gacgagaggg gacgcgaaac ggcgcatggt cgggccttcc tggatggcga
     8761 tgggtcgagc gaacgcaacg atagcgcaaa cggacgaccg cggccgagca gg
//
LOCUS       DASVLN010000066         8364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_218000, whole genome shotgun sequence.
ACCESSION   DASVLN010000066 DASVLN010000000
VERSION     DASVLN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218000"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VF453_01470"
     CDS             <1..392
                     /locus_tag="VF453_01470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX7686338.1"
                     /translation="LVVMVGTAVTVDALDPDGRFLGGLILPGFGLMLRALEMGTAGLK
                     VPTGETVDFPTNTSDALMSGGVNAIAGAVERQYRRLRARTGVEPRVLVTGGAAVKLAP
                     ALDLDLEIVDGLIFEGLLGIEAARTAR"
     gene            complement(426..1025)
                     /locus_tag="VF453_01475"
     CDS             complement(426..1025)
                     /locus_tag="VF453_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7686339.1"
                     /translation="MKQTLIAGAVAAAATLIASPAFAQTYLGAGIGVNHSNRACGAVD
                     ATGASVDSCDKNATAVKIYGGYQLPGTPWAGELTYYDLGRYKASGAFGDAHAKGQYWG
                     IGGAYRPDFGNGWGGVARIGAAYGQGKVDYSLAGSTSGSDTRNDWHPYYGLGVNYALT
                     KQVKLEADWDNTRLTTRVPGFSAATSTVNSFTLGASYAF"
     gene            complement(1253..2614)
                     /gene="hslU"
                     /locus_tag="VF453_01480"
     CDS             complement(1253..2614)
                     /gene="hslU"
                     /locus_tag="VF453_01480"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX7686340.1"
                     /translation="MTQSTMTPQEIVSELDRHIVGQHEAKRAVAIALRNRWRRQQVEP
                     KLRHEITPKNILMIGPTGVGKTEIARRLARLADAPFVKVEATRFTEVGYVGKDVDTIV
                     RDLVEVAVKEERERAVKRHRVQAEDAAEERVLDILVPPSRASGSGSMGFGGAAPVPAP
                     ADNTARQTMRKRLREGQLDDKEIEVELVEAKSGPDILTPPGMEDMAEQLKGMFSQMGG
                     QRTKARKLRIAEALKLLAEEEAAKRVNEEEIRAAAVAHAEQNGIVFIDEIDKVAARSE
                     GQGADVSRQGVQRDLLPLVEGTTVNTKYGMVKTDHVLFIASGAFHFARPSDLIPELQG
                     RFPIRVELKALSVDDFESILTSTHASLVKQYQALLATEGVTIEFGADSVRRIAQIAFE
                     VNERTENIGARRLATVMERLLEDISYDAPNRGGQTVTISADTVSARLAELARDEDLSR
                     FIL"
     gene            complement(2646..3182)
                     /gene="hslV"
                     /locus_tag="VF453_01485"
     CDS             complement(2646..3182)
                     /gene="hslV"
                     /locus_tag="VF453_01485"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX7686341.1"
                     /translation="MEPFHGTTIVSVRRGQQVAIGGDGQVTLGNIVVKATARKVRKLY
                     REQVLAGFAGATADAFTLFERFEAKLEKHQGHLVRAAIDLTRDWRTDRVLRRLEAMLA
                     VADRTASLIISGNGDVLEPEGGIIAIGSGGAYAQAAAIALTQHTELAPRDVVKRSLEI
                     AGDLCIYTNQNHTIEVLE"
     gene            complement(3320..5314)
                     /locus_tag="VF453_01490"
     CDS             complement(3320..5314)
                     /locus_tag="VF453_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7686342.1"
                     /translation="MSDKDEGGGGGLFRRMARKVAAPARELVGMPARSSGLGEGDARP
                     TEFDKAEMKAMIERKRRNDYVRKRELDTLRRIRREGLSADQAQALTMQSSRMDENTEA
                     RSTQSSVQSSGIKAKIDAIERQMVGASSGVHAGGVQAGSGTPSKPAAPRVAPTLPPGP
                     PPPILTATIFDEEAAPTVPVTLSTTGSDENETVARLDGGPVSLPPLNMPAPGASPEGD
                     TKPVGLDGRAFANTETYPPTSAPAPLSIRQPLEFTPTPMSAAPVISHGVSPNPYAAGA
                     PAPAPRAQPAAQATVDLGGGERVEVSEVVHDDALDEAVIAFAGGDFQACERALQALVG
                     PRGERRDHDETWLVLFDLYRAIGQQSKFETVTMEYVERFHRSAPQWFSLPKMLAEATK
                     APQAEERLGGGIGWVCPPKLDPEAVTRLDALSMQLPSPWVLDWSALQSIDVEAAGRLR
                     KLFAGWANQALTMHWQGGEKLFSVLQDTAPVGVRDADPVFWMLRLEALRLAHRPDQFD
                     EVAIDYCVTYEVSPPSWENARCKALLKDNAQNTRTLSLSVVGEATTSFMDTSMMGEDS
                     TPAPVQITSIELAGQLSGDLGELLGKLTTQVGESRVVRISCALLMRVDFVAAGDLLNW
                     VAMRHSEGREIVFHDTHRLVALMFGAMGINDTARVQLRKG"
     gene            complement(5381..6016)
                     /gene="dksA"
                     /locus_tag="VF453_01495"
     CDS             complement(5381..6016)
                     /gene="dksA"
                     /locus_tag="VF453_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX7686343.1"
                     /translation="MTKTSAKPTAATFDADDAPKSRAKPTPAGTKASPAADGAAGPPV
                     KKPSSKAESEPMNAAKSTDTKLATAWKTKAGKDLTEAELLAMPESEYMGEKQLEFFRN
                     RLQALKDDLLSNAGETTEHLREDTSIVPDPADRATIEEEHALELRTRDRERKLLKKIS
                     QSIGRIDAGDYGYCDETGEPIGLGRLLARPTATLSLEAQQRREMKQKMFGD"
     gene            complement(6191..7237)
                     /locus_tag="VF453_01500"
     CDS             complement(6191..7237)
                     /locus_tag="VF453_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7686344.1"
                     /translation="MSDKLIPATILTGFLGAGKTTLLKRVLTETHGQKIAVIENEFGE
                     ENIDNEILVSDTTEQIMQLSNGCVCCTIREDLRATLALLAERRRKGELHFERVVIETT
                     GLADPGPVAQTFFMDDEIAETYLLDSILTLVDAKHANEQLDTRQEARLQVGFADQIFI
                     SKADLVDAAALDALQHRLRHMNPRAPQQKVHFGEVPISSVFGLKGFNLNAALEIDPEF
                     LNAGAHDHDHDHPHHDHAEGEACDHPHHHHHDDDVKSFVFRSDKPFNPNKLEDFLGAI
                     VQVYGPKMLRYKGVLYMKGHDRKVIFQGVHQMMGSDLGPKWAPGEKKTNKMVFIGLDL
                     PREILLQGLEGCLA"
     gene            7388..8293
                     /locus_tag="VF453_01505"
     CDS             7388..8293
                     /locus_tag="VF453_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEX7686345.1"
                     /translation="MSDPQPGRPGRPSRAGAEADKRSLFERVVEFLSPGPDSRDELIE
                     TLAEAEQRSLIEPESRAMLEGVLRMADRTAGDVMVAAPRMDLLDIDAPYDELLAAVIG
                     TGHSRFPVYDGQRENIIGTLMAKDLLKLQRAPELNLRTLLRPAVFVPESKGLNELLRE
                     FRSNRNHLAIVIDEFGNTAGLITIEDVLEEIVGEIEDEFDEADADSGGTYVLADGSLR
                     VAGDATIAEVNQAFAVTLPEDAFDTIGGYVAHEIGHVPRRGEAFRIGGLQFSVMLTRG
                     GAVRWFKVTRVPAEPAAPHPDELGG"
BASE COUNT         1105 a         2803 c         3044 g         1412 t
ORIGIN      
        1 cgctggtcgt gatggtcggc acggcggtga ccgtcgatgc cctcgacccc gacggccgct
       61 tcctcggcgg cctcatcctg cccggcttcg ggctgatgct gcgcgcgctg gagatgggca
      121 ccgccgggct gaaggtgccc accggcgaga ccgtcgattt cccgaccaat accagcgacg
      181 cgctgatgtc gggcggcgtc aacgcgatcg ccggggcggt cgagcgccag taccgccggc
      241 tgcgcgcgcg caccggcgtc gagccgcgcg tgctggtcac cggcggcgcc gcggtcaagc
      301 tggcgcccgc gctggacctc gacctcgaga tcgtcgacgg cctgatcttc gagggcctgc
      361 tgggcatcga ggctgcacgc accgcccgct gaggccgcgg gccgcgcggt gccggggcgc
      421 tcgcgtcaga acgcgtacga cgcgccgagc gtgaagctgt tgaccgtcga ggtcgcggcg
      481 ctgaagcccg gcacgcgggt cgtcagccgg gtgttgtccc agtcggcctc gagcttgacc
      541 tgcttggtca gcgcgtagtt cacacccagg ccgtagtacg ggtgccagtc gttgcgcgtg
      601 tcggagccgc tggtcgatcc cgccaggctg tagtcgacct tgccctggcc gtaggccgcg
      661 ccgatgcggg cgacgccgcc ccaaccgttg ccgaagtccg gacgataggc gccgccgatg
      721 ccccagtact ggcccttggc atgcgcgtcg ccgaacgcgc ccgaggcctt gtagcggccg
      781 aggtcgtagt aggtcagctc gcccgcccaa ggcgtgccgg gcagctggta gccgccgtag
      841 atcttcacgg cggtcgcgtt cttgtcgcag ctgtcgaccg aggcgccggt ggcgtcgacg
      901 gcgccgcagg cgcgattcga atggttcacg ccgatcccgg cgccgaggta ggtttgcgcg
      961 aacgccgggc tggcgatcag ggtggcggcg gcggcaacgg cgcctgcgat cagggtctgc
     1021 ttcacagtga ttcctcactc aagaaaggta ctcccggggg agcgatgagg atcagtctag
     1081 ctaagcgttt tccctgattc gctgaatttg tcggtcagga gccacacaaa atggtgaatc
     1141 tgtggtaaag gcgcctttac gcttcgaatt gtgtcgccgc ggaacttctt ccggcgatga
     1201 aaaatggcgc cggcctcgcg gcaagcgcca ttggttgcaa gcgagcggtc actcagagga
     1261 tgaagcgcga cagatcctcg tcgcgcgcga gctccgccag ccgcgcgctg accgtgtcgg
     1321 cgctgatcgt gacggtctgg ccgccgcggt tgggggcgtc gtagctgatg tcctccagca
     1381 gccgctccat cacggtcgcc aggcgacgcg cgccgatgtt ctcggtgcgc tcgttgacct
     1441 cgaaggcgat ctgcgcgatc cggcgcacgc tgtcggcgcc gaactcgatg gtcacgccct
     1501 cggtcgcgag cagcgcctgg tactgcttga ccaggctcgc atgggtgctc gtgaggatgg
     1561 actcgaaatc gtccaccgac agcgccttca gctcgacgcg gatggggaag cggccctgca
     1621 gctccgggat caggtcgctc gggcgggcga agtggaatgc cccggaggcg atgaacagga
     1681 cgtggtcggt cttgaccatc ccgtacttcg tgttgaccgt cgtgccctcg accagcggca
     1741 ggaggtcgcg ctggacgccc tggcgcgaga cgtccgcgcc ctggccctca gagcgcgcgg
     1801 ccaccttgtc gatctcgtcg atgaagacga tgccgttctg ctccgcgtgc gcgacggcgg
     1861 ccgcgcggat ctcctcctcg ttgacgcgct tggcggcttc ctcctcggcg agcagcttca
     1921 gcgcctcggc gatgcgcagc ttgcgcgcct tggtgcgctg gccgcccatc tgcgagaaca
     1981 tgcccttcag ctgctcggcc atgtcctcca tgccgggcgg cgtcaggatg tcggggccgg
     2041 acttggcctc gaccagctcg acctcgatct ccttgtcgtc cagctggccc tcgcgcaggc
     2101 gcttgcgcat cgtctggcgc gccgtgttgt ccgcgggggc cggcacgggc gcggcgccgc
     2161 cgaagcccat cgagccggag cccgaggcgc gcgacggcgg cacgaggatg tcgagcacgc
     2221 gttcctcggc ggcgtcctcg gcctgcacgc ggtggcgctt gacggcgcgc tcgcgctcct
     2281 ccttcacggc gacctcgacg aggtcgcgca cgatggtgtc gacgtccttg cccacgtagc
     2341 cgacctcggt gaagcgcgtg gcctcgacct tgacgaacgg cgcgtccgcc agccgcgcga
     2401 gacggcgcgc gatctccgtc ttgccgacgc cggtcgggcc gatcatgagg atgttcttgg
     2461 gcgtgatctc gtggcgcagc ttcggctcga cctgctgccg gcgccagcgg ttgcgcagcg
     2521 cgatcgcgac ggcgcgcttg gcctcgtgct ggcccacgat gtggcggtcg agctcggaga
     2581 cgatctcctg cggggtcatg gtcgactggg tcattggaat ccctcgccgg gcgaatacgt
     2641 cgtcgtcact cgagtacctc gatcgtgtgg ttctggttcg tgtagatgca caggtcgccc
     2701 gcgatctcga gcgagcgctt gacgacgtcg cgcggggcga gctcggtgtg ctgcgtgagc
     2761 gcgatcgccg cggcctgggc ataggcgccg ccggagccga tggcgatgat gccgccctcg
     2821 ggctcgagca cgtcgccgtt gccgctgatg atcagcgacg ccgtgcggtc ggccacggcg
     2881 agcatcgcct cgaggcggcg caggacgcgg tcggtgcgcc agtcgcgcgt gaggtcgatc
     2941 gcggcacgca cgaggtgccc ctggtgcttc tcgagcttgg cctcgaagcg ctcgaacagc
     3001 gtgaaggcgt cggcggtggc gccggcgaag cccgccagca cctgttcccg gtagagcttg
     3061 cgcaccttgc gtgcggtcgc cttgacgacg atgttgccga gggtgacctg gccgtcgccg
     3121 ccgatcgcga cctgctggcc gcggcgcacg ctgacgatgg tggtgccgtg gaagggttcc
     3181 atgatgggat gcgggaaagt cggccgccgg gcggccggat gagggcaggt agggattgtc
     3241 gggcgccgat caagtcgcgg ccgcggcttg cccgcgtcgc gcccggggtc gcgcgccggt
     3301 cgggcgaccg gccggccggt cagcccttgc gcagctggac gcgcgcggtg tcgttgatgc
     3361 ccatcgcgcc gaacatcagc gcgacgaggc ggtgcgtgtc gtggaagacg atctcgcggc
     3421 cttccgaatg gcgcatcgcg acccagttga gcaggtcgcc ggcggccacg aaatccaccc
     3481 gcatcagcag cgcacaggag atgcgcacga cgcgcgactc gccgacctgc gtggtcagct
     3541 tgccgagcag ctcgcccagg tcgcccgaca gctggccggc gagctcgatg gaggtgatct
     3601 gcaccggcgc gggcgtggag tcctcgccca tcatcgacgt gtccatgaag ctggtcgtgg
     3661 cctcgccgac gaccgacagc gacagggtgc gcgtgttctg ggcgttgtcc ttgagcagcg
     3721 ccttgcagcg cgcgttctcc cagctgggcg gcgagacctc gtaggtcacg cagtagtcga
     3781 tcgcgacctc gtcgaactgg tcgggccggt gcgcgaggcg caaggcctcc aggcgcagca
     3841 tccagaagac cggatcggcg tcgcgcacgc ccaccggggc ggtgtcctgc agcacggaga
     3901 acagcttctc gccgccctgc caatgcatcg tcagggcctg gttggcccag cccgcgaaca
     3961 gcttgcgcag gcggccggcg gcctcgacgt cgatggactg cagcgccgac cagtccagca
     4021 cccagggcga gggcagctgc atcgacagcg cgtccaggcg cgtgacggcc tcgggatcga
     4081 gcttcggcgg gcacacccag ccgatgccgc cgcccagccg ctcctcggcc tgcggcgcct
     4141 tggtcgcctc ggcgagcatc ttgggcagcg agaaccactg cggcgccgag cgatggaaac
     4201 gctcgacgta ctccatcgtg acggtctcga acttcgactg ctggccgatc gcgcggtaca
     4261 ggtcgaacag cacgagccag gtctcgtcgt ggtcgcggcg ctcgccgcgc ggacccacga
     4321 gcgcctgcag cgcgcgctcg caggcctgga agtcgccgcc ggcgaaggcg atgaccgcct
     4381 cgtcgagcgc gtcgtcgtgg acgacctcgc tgacctcgac gcgctcgccg ccgccgaggt
     4441 cgacggtcgc ctgcgcggcc ggctgggcgc gcggcgcggg cgccggcgcg ccggcggcgt
     4501 acgggttggg cgagacgccg tgcgagatga cgggcgccgc ggacatcggc gtcggcgtga
     4561 attccagcgg ttggcggatc gacagcggtg ccggcgccga cgtcggcggg taggtctcgg
     4621 tgttggcgaa cgcgcggccg tcgaggccga cgggcttggt gtcgccctcc gggctcgcgc
     4681 cgggcgcggg catgttcagc ggaggcaggc tcaccgggcc gccgtccagg cgggcgacgg
     4741 tctcgttctc gtcgctgccg gtcgtgctca gcgtgacggg caccgtgggg gcggcttcct
     4801 cgtcgaagat cgtcgcggtg aggatgggcg gcggcgggcc gggcggcagg gtgggcgcga
     4861 cgcgcggcgc ggccggcttg gacggcgtgc cggagccggc ctggacgccg ccggcgtgca
     4921 cgccggagct cgcgcccacc atctggcgct cgatcgcgtc gatcttcgcc ttgatgccgc
     4981 tgctctgcac cgaggactgc gtcgagcgcg cctcggtgtt ctcgtccatg cgcgaggact
     5041 gcatcgtgag ggcctgcgcc tggtcggcgg acagcccctc gcggcggatg cggcgcagcg
     5101 tgtcgagctc gcgcttcctc acgtagtcgt tgcggcgctt gcgctcgatc atggccttca
     5161 tctcagcctt gtcgaactcc gtcggccgcg cgtcgccttc gcccaggccg ctgctgcggg
     5221 cgggcatgcc caccagctcg cgcgccggcg cggcgacctt gcgcgccatg cgccggaaca
     5281 ggcctccgcc gccgccttcg tctttatcgg acatgggcag gctgtccgag cccgggttgc
     5341 gggcgcggcg gggcagggcg gtggttcgag cgcggcaggg tcagtccccg aacatcttct
     5401 gcttcatctc gcgacgctgc tgcgcctcga gcgagagcgt cgcggtcggg cgcgccagca
     5461 ggcggcccag gccgatcggc tcgccggtct cgtcgcagta gccgtagtcg cccgcgtcga
     5521 tgcggccgat ggactgcgag atcttcttca gcagcttgcg ctcgcggtca cgggtgcgca
     5581 gctccagggc gtgctcctcc tcgatcgtgg cgcggtcggc cggatcgggg acgatggagg
     5641 tgtcctcgcg caggtgctcc gtggtctcgc cggcgttgga cagcaggtcg tccttgagcg
     5701 cctgcaggcg gttgcggaag aactcgagtt gcttctcgcc catgtactcg ctctcgggca
     5761 tggcgagcag ctcggcctcg gtcagatcct tgccggcctt ggtcttccag gccgtggcga
     5821 gcttcgtgtc ggtggatttt gcagcgttca tcggttcgga ttcggccttt gaggaaggct
     5881 tcttgacggg agggccggcg gcaccgtccg ccgcgggaga cgccttggtg ccggcagggg
     5941 tgggcttggc acgcgacttc ggcgcgtcat ccgcgtcgaa cgtcgctgcg gtgggcttgg
     6001 ccgaggtctt ggtcacggtc gatctcctcg cgtcggatcg ctcggaaggc tgttataccc
     6061 gctgccggca accgttgaaa gcggtacgga gcggggctga ccttgcaaaa aaggcgggcc
     6121 gattgtagcc acgatgtccg atgccgtagt catggatttc gtcacgcttt cccggccgca
     6181 ggcgcatttt tcaggcgagg cagccttcca ggccctggag caggatctcg cgcggcaggt
     6241 ccaggccgat gaagaccatc ttgttggtct tcttctcgcc cggggcccac ttcggcccga
     6301 ggtcgctgcc catcatctgg tgcacgccct ggaagatcac cttgcggtcg tggcccttca
     6361 tgtagagcac gcccttgtac cgcagcatct tcgggccgta gacctggacg atcgcgccga
     6421 ggaagtcctc cagcttgttc gggttgaacg gcttgtccga ccggaacacg aaggacttca
     6481 cgtcgtcgtc gtggtgatgg tggtgcggat ggtcgcaggc ctcgccctcg gcgtggtcgt
     6541 gatgagggtg gtcgtggtca tggtcgtgcg ccccggcgtt caggaactcg gggtcgatct
     6601 cgagcgccgc gttcaggttg aagcccttga ggccgaacac gctggagatc ggcacctcgc
     6661 cgaagtgcac cttctgctgc ggcgcgcgcg ggttcatgtg gcgcaggcga tgctgcagcg
     6721 cgtccagcgc cgcggcgtcg accaggtcgg ccttgctgat gaagatctgg tcggcgaacc
     6781 cgacctgcag gcgcgcctcc tggcgggtgt cgagctgctc gttggcatgc ttggcgtcga
     6841 ccagcgtcag gatcgagtcg agcaggtagg tctctgcgat ctcgtcgtcc atgaagaagg
     6901 tctgggcgac cgggccggga tcggcgaggc cggtcgtctc gatgacgacg cgctcgaagt
     6961 gcagctcgcc cttgcggcgg cgctccgcca gcagggccag cgtggcgcgc aggtcctcgc
     7021 ggatcgtgca gcagacgcag ccgttggaca gctgcatgat ctgctcggtg gtatccgaga
     7081 cgaggatctc gttgtcgatg ttctcctcgc cgaattcgtt ctcgatcacg gcgatcttct
     7141 ggccgtgcgt ctccgtgagg acgcgcttga gcagcgtggt cttgcccgcg ccgaggaagc
     7201 cggtgaggat ggtggcggga atgagcttgt cggacatggg ttcttcggag gtggtggtcg
     7261 ccggcaggaa tgatgccgag gccggcgggg cagggcatct gggggcggcg aactctggtt
     7321 caaggccgcc gcccttgtca aggtaatcgg gtattctcgc ggctcgtcca ctcccgacac
     7381 cgccatagtg tctgatcccc agcccggtcg acccggccgc ccgtcccgcg cgggggccga
     7441 ggccgacaag cgctccctgt tcgagcgcgt ggtcgagttc ctgtcgccag gccccgactc
     7501 gcgcgacgag ctgatcgaaa ccctggccga ggccgagcag cgttcgctga tcgaaccgga
     7561 atcccgggcc atgctcgagg gcgtgctgcg catggccgac cgcaccgcgg gcgacgtgat
     7621 ggtcgccgcg ccgcgcatgg atctgctcga catcgacgcg ccctacgacg agctgctcgc
     7681 cgccgtcatc ggcacgggcc attcccgctt cccggtctac gacggccagc gcgagaacat
     7741 catcggcacg ctgatggcca aggacctgct gaaattgcag cgcgccccgg agctgaacct
     7801 gcgcacgctg ctgcggccgg cggtcttcgt gccggagtcc aagggcctca acgagttgct
     7861 gcgcgaattc cgcagcaacc gcaaccacct cgcgatcgtc atcgacgagt tcggcaacac
     7921 ggcgggcctg atcacgatcg aggacgtgct cgaggagatc gtcggcgaga tcgaggacga
     7981 gttcgacgag gcggatgccg actccggcgg cacctacgtg ctggccgacg gcagcctgcg
     8041 cgtggccggc gacgccacga tcgcggaggt caaccaggcc ttcgccgtca ccctgccgga
     8101 ggacgccttc gacacgatcg gcggctacgt cgcgcacgag atcggccacg tgccgcgccg
     8161 cggcgaggcg tttcgcatcg gcgggctgca gttctccgtg atgctcaccc gtggcggcgc
     8221 cgtgcgctgg ttcaaggtca cgcgcgtgcc ggccgagccc gcggcgccgc atcccgacga
     8281 actcggcgga tgagcgggca ctcgcttcgt ggccgggcgc ggctgccgct gctgcccggc
     8341 tgggccggcg tgctcgccgc ggcg
//
LOCUS       DASVLN010000067         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_164916, whole genome shotgun sequence.
ACCESSION   DASVLN010000067 DASVLN010000000
VERSION     DASVLN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164916"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /gene="flhF"
                     /locus_tag="VF453_01510"
     CDS             <1..1126
                     /gene="flhF"
                     /locus_tag="VF453_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEX7686346.1"
                     /translation="RAAMPAQPQASATMAAPQRPAMRAERAQPEFAHAPVLHDEFAEP
                     VHAIHAPQAVDAAARQSQMDMVSELRSMKGLIEERFGALAFMEKLQRQPRQALLAQKL
                     LDVGFSPALIRKMVDALPAGVTDENAWAAGVLERNLLTNEAEMAMEDQGGVYALIGST
                     GVGKTTTTAKIAAGFAARHGAANLGLITLDAYRVGAHEQLRAYGRILGVPVHTAHDRA
                     SLEDLLELLAGKKMVLIDTAGMAQRDGRTRELLEMLAHRSIQKVLVVDASSQGETIDD
                     VIVSWKASQCKGVILSKIDEAVKLAPALDALIRHKLRNIGVANGQRVPEDWHRLSANA
                     LVHRALKGGGSAAYRMDPQDVHLVFAHAGMNTSAAAGLRV"
     gene            1183..>1940
                     /locus_tag="VF453_01515"
     CDS             1183..>1940
                     /locus_tag="VF453_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HEX7686347.1"
                     /translation="MTRRSSPPTDQADGLRRMFASSRAVLVPVVANPHVEHHGAVLAR
                     LAAVFAEQGLNTLVVDAADTAPEPGELAFIDLAACVEPLAPQLAYLAARGLPMQHVDT
                     RGSCASFIPRVTSVVADIDVVVLHAEARVLARMLGHRESRPVLLAGSEGESITHAYAA
                     LKLLVQRLQVMSFDLVTPTMRGRGPTPAQVVDRVADCADRFLGAALHDSAAIDPDLAS
                     GAPAGPDLMRLACAQLALAEGDADGIPTLTQALP"
BASE COUNT          279 a          743 c          668 g          250 t
ORIGIN      
        1 ccgcgccgcg atgcccgcgc agccgcaggc gtccgcgacg atggccgccc cgcagcgtcc
       61 cgcgatgcgc gccgagcgcg cgcagcccga gttcgcgcat gcgccggtgc tgcacgacga
      121 gttcgccgag ccagtccacg cgatccacgc gccgcaggcc gtcgacgccg ccgcgcgcca
      181 gagccagatg gacatggtga gcgagctgcg ctcgatgaag ggcctgatcg aggagcgctt
      241 cggtgccctg gccttcatgg agaagctgca gcgccagccg cgccaggcac tgctcgcgca
      301 gaagctgctg gacgtcggct tctcgccggc gctgatccgc aagatggtcg atgcgctgcc
      361 cgcgggcgtc accgacgaga acgcgtgggc ggccggcgtc ctcgagcgca acctgctcac
      421 gaacgaggcc gagatggcga tggaggacca gggcggcgtg tacgcgctga tcggctccac
      481 cggcgtcggc aagacgacga ccaccgccaa gatcgccgcc ggcttcgccg cgcgccacgg
      541 cgccgccaac ctgggcctga tcacgctgga cgcctaccgc gtcggcgccc acgagcagct
      601 gcgcgcctac ggccgcatcc tcggcgtgcc ggtgcacacc gcgcacgacc gcgcctcgct
      661 ggaggacctg ctggagctgc tggcgggcaa gaagatggtg ctgatcgaca cggccggcat
      721 ggcccagcgc gacggccgca cccgcgagct gctcgagatg ctggcccacc gctcgatcca
      781 gaaggtgctg gtggtcgacg cctcgtcgca gggcgagacc atcgacgacg tgatcgtcag
      841 ctggaaggcc tcgcagtgca agggcgtgat cctgtccaag atcgacgagg cggtgaagct
      901 ggccccggcg ctggacgcgc tgatccgcca caagctcagg aacatcggcg tggccaacgg
      961 ccagcgcgtg cccgaggact ggcaccgcct gtcggccaac gcgctcgtgc accgcgcgct
     1021 caagggcggc ggcagcgcgg cctaccggat ggatccgcag gacgtgcacc tcgtgttcgc
     1081 gcatgccggc atgaacacct cggccgccgc cggcctgcgt gtctgaccaa gccggtcctt
     1141 ttccgaagat cgaccccacg cccgcatcga catagtcatc ccatgacccg tcgcagctct
     1201 cctcccaccg accaggccga cggcctgcgc cgcatgttcg catcctcgcg ggcggtgctc
     1261 gttccggtgg tcgcgaaccc gcacgtggag caccacggcg cggtgctcgc gcgcctggcg
     1321 gccgtgttcg cggaacaggg cctgaacacg ctggtcgtcg acgccgccga cacggcgccc
     1381 gagcccggcg agctcgcctt catcgacctg gccgcctgcg tcgagccgct cgcgccccag
     1441 ctcgcctacc tcgcggcacg cgggctgccg atgcagcacg tggacacgcg cggctcctgc
     1501 gcgagcttca tcccgcgcgt gacgagcgtc gtcgccgaca tcgatgtcgt cgtcctgcac
     1561 gccgaggcgc gcgtgctcgc ccgcatgctg ggccatcgcg aatcgcgccc ggtgctgctg
     1621 gccggcagcg aaggcgagag catcacccac gcctacgccg cgctcaagct gctcgtgcag
     1681 cggctgcagg tcatgagctt cgacctcgtc acgccgacga tgcgcggccg cgggccgacg
     1741 ccggcgcagg tcgtggaccg ggtcgcggac tgcgccgacc gcttcctcgg cgccgcgctg
     1801 cacgactccg cggcgatcga tcccgacctc gcgtcgggcg cgcccgccgg gcccgacctg
     1861 atgcgcctcg cgtgcgcgca gctcgcgctc gccgaaggcg acgccgacgg catcccgacg
     1921 ctcacgcagg cgctccccga
//
LOCUS       DASVLN010000068         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_57043, whole genome shotgun sequence.
ACCESSION   DASVLN010000068 DASVLN010000000
VERSION     DASVLN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57043"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF453_01520"
     CDS             complement(<1..225)
                     /locus_tag="VF453_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7686348.1"
                     /translation="MDTRKIEALWSHVHGLGVLRSAGSFTAAAQRLGLSKAAMSQRVA
                     ELERAAGVPLVRRTTRSVRLTEAGERLVEAT"
     gene            362..1882
                     /locus_tag="VF453_01525"
     CDS             362..1882
                     /locus_tag="VF453_01525"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7686349.1"
                     /translation="MPTPAAFTSTAPVGHFIGGERALPAGARTQAVWNPATGAVARQL
                     HLGTAADVDAAVGAARRAFPAWADTPPLRRARVLNQFLALLNQHKDELAAMITAEHGK
                     VFSDAQGEVARGIDVVEFACGIPQLLKGDYTEQVSTGIDNWTMRQPLGVVAGVTPFNF
                     PFMVPCWMFPVAIACGNTFVLKPSERDPSASLFIAELLQRAGLPDGVFNVVQGDKVVV
                     DALLAHPDVHALSFVGSTPIAQYIYETGARHGKRVQALGGAKNHMVVMPDADIDQAVD
                     ALVGAAYGSAGERCMAISVAVLVGDVADRLLPKLAQRARSLVVDNGMNPAAEMGPIVT
                     KQALERIEGYIALGVEEGATLVVDGRGHRVPGHEDGFFTGGTLFDHVTPQMRIYREEI
                     FGPVLACVRMPDFASAVELVNAHEFGNGVACFTSDGNVAREFARRIQVGMVGINVPIP
                     VPMAWHGFGGWKKSLFGDMHAYGEEGVRFYTKQKSVMQRWPAGIAKGAEFAMPTHR"
     gene            2009..>2157
                     /locus_tag="VF453_01530"
     CDS             2009..>2157
                     /locus_tag="VF453_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7686350.1"
                     /translation="MTAAKVLLVTGASRGIGAACAEKAAAAGWDVAVHFNRDARAAAR
                     VADSVR"
BASE COUNT          307 a          791 c          758 g          301 t
ORIGIN      
        1 cgtggcctcg acgaggcgct cgcccgcctc ggtcaggcgc acgctgcgcg tcgtgcgccg
       61 caccagcggc acgcccgcgg cgcgctccag ctcggcgacg cgctggctca tcgccgcctt
      121 gctcagaccc aggcgctgcg ccgccgcggt gaagctgccg gcgctgcgca gcacgcccag
      181 gccgtggacg tgcgaccaga gcgcctcgat cttgcgggtg tccatcgtgg ttgttccgtg
      241 attcgatgtg acgattgttc actcaaacga acagtgattc cagccgggcg gacttccccc
      301 gccgcgccgc gatgcctaga ctggcgcggt cgcatcgacc atttcgccag gagacaaacg
      361 catgcccacg cctgccgcct tcacgtccac cgcgcccgtc ggccatttca tcggcggcga
      421 gcgcgcgctg ccggccgggg cgcgcacgca ggccgtgtgg aacccggcca cgggcgcggt
      481 cgcgcgccag ctgcacctgg gcacggccgc cgacgtggac gccgccgtcg gtgccgccag
      541 gcgggcattt ccggcctggg ccgacacgcc cccgctgcgg cgcgcgcgcg tgctcaacca
      601 gttcctcgcg ctgctgaacc agcacaagga cgagctcgcc gcgatgatca ccgccgagca
      661 cggcaaggtc ttctccgatg cgcagggcga ggtggcgcgc ggcatcgacg tcgtcgagtt
      721 cgcctgcggc atcccgcagc tgctcaaggg cgactacacc gagcaggtct ccacgggcat
      781 cgacaactgg acgatgcgcc agccgctggg cgtcgtcgcc ggggtgacgc cgttcaactt
      841 cccgttcatg gtgccgtgct ggatgttccc ggtcgcgatc gcctgcggca acaccttcgt
      901 gctgaagccc agcgagcgcg acccgagcgc gagcctgttc atcgccgagc tgctgcagcg
      961 cgccggcctg cccgacggcg tcttcaacgt cgtccagggc gacaaggtcg tcgtcgacgc
     1021 gctgctcgcg catccggacg tccacgcgct gtccttcgtc ggctcgaccc cgatcgcgca
     1081 gtacatctac gagaccggcg cccgccacgg caagcgggtg caggcgctgg gcggcgcgaa
     1141 gaaccacatg gtcgtcatgc ccgacgccga catcgaccag gccgtcgacg cgctcgtcgg
     1201 cgcggcctac ggctcggcgg gcgagcgctg catggcgatc tcggtggcgg tgctggtggg
     1261 cgacgtcgcc gaccgcctcc tgcccaagct cgcgcagcgc gcgcggtcgc tggtcgtcga
     1321 caacggcatg aacccggccg ccgagatggg gccgatcgtg acgaagcagg cgctcgagcg
     1381 catcgagggc tacatcgcgc tcggcgtcga ggaaggcgcc acgctggtcg tcgacggccg
     1441 cggccaccgg gtgccgggcc acgaggacgg cttcttcacc ggcggcacgc tgttcgacca
     1501 cgtgacgccg cagatgcgca tctacaggga agagatcttc ggcccggtgc tcgcctgcgt
     1561 gcgcatgccc gacttcgcgt cggccgtgga gctcgtcaac gcgcacgagt tcggcaacgg
     1621 cgtggcctgc ttcacgagcg acggcaacgt cgcgcgcgag ttcgcgcgcc gcatccaggt
     1681 cggcatggtc ggcatcaacg tgccgatccc ggtgcccatg gcctggcacg gcttcggcgg
     1741 ctggaagaaa agcctgttcg gcgacatgca cgcgtacggc gaggaaggcg tgcgcttcta
     1801 cacgaagcag aagagcgtga tgcagcgctg gcccgccggc atcgccaagg gcgccgaatt
     1861 cgcgatgccc acgcacaggt gaggccgcca tcgcgctggc gcgcgggctt gcgagctcgc
     1921 ggcaagcatc ccgcgagccg ctccggcggg gtagcgccgg tgccgcggac gcgcgacatc
     1981 tccgggtcgg catgctagaa cggcgaacat gaccgccgcg aaagtcctcc tcgtcaccgg
     2041 cgccagccgc ggcatcggtg cggcctgcgc cgagaaggcc gcggcggcgg gctgggacgt
     2101 cgccgtccac ttcaaccgcg acgcccgggc cgccgcgcgc gtcgccgact cggtgcg
//
LOCUS       DASVLN010000069         1939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_487174, whole genome shotgun sequence.
ACCESSION   DASVLN010000069 DASVLN010000000
VERSION     DASVLN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487174"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VF453_01535"
     CDS             complement(<1..388)
                     /locus_tag="VF453_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686351.1"
                     /translation="MTTRPLPHGHHLRALDARDLPALLALHAAVMRALPDPGLFRLFG
                     GPERFLSDHLGARGESLGAFAGERLVGYGSVTRPAAGDLDNYARDLGWTPERAGRVAL
                     LSAAMVDPGSRGQGLHRALIAARIALA"
     gene            complement(385..1398)
                     /locus_tag="VF453_01540"
     CDS             complement(385..1398)
                     /locus_tag="VF453_01540"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7686352.1"
                     /translation="MMRRRELLAVALAPALGRAGSRPWRIYAITYRGRTDVERGFADY
                     FAARGLPVEITWRDIALDPSRLPALVDEIRRERPDLVYTWGTGVTLGVAGRWDEADAS
                     RHLRGIPIVFSLVASPVGAGIVRDLRSPGRPVTGVSHMAGLEAQVQAMASYRPFARLG
                     MLYSSNEPNSAAVLRELQALGRRRGFEVLARPFRVDAAGQPGANGAADIVHGLQAAGA
                     QWLYMPPDSFLGTQARAVVAPAALEARLPAFASTEQLMASGALLGLVSRYYNVGQFAA
                     YKAERILSGQAPAQGIPVETLTRFSLQIDLRVARRLDMLPPLEMFNYAELIGDAGKTP
                     PSS"
     gene            complement(1395..>1939)
                     /locus_tag="VF453_01545"
     CDS             complement(1395..>1939)
                     /locus_tag="VF453_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7686353.1"
                     /translation="LAALPAKLILAGSCFYLVPVFVARADASPSVAGRAIMLYAVALV
                     LVLPQATRWVERGVPLARLVGAGLCLSSLGGFALLGAGGVASVYAVTALLGLGQGLSI
                     AAQSSLLSQVCAAEIEAHGSGPVFGAYRLVERLGNACGPLVAGGLAVLAGPAAAFVGM
                     ATIVLGCGLCFALLARRVAR"
BASE COUNT          287 a          727 c          741 g          184 t
ORIGIN      
        1 gtgcgagcgc gatgcgcgcg gcgatcagcg cgcggtgcag tccctggcct cgggagccgg
       61 gatcgaccat cgcggccgac agcagcgcca cgcggccggc gcgctcgggc gtccagccga
      121 ggtcgcgcgc gtagttgtcg aggtcgccgg cggccggccg ggtcaccgag ccgtagccga
      181 cgaggcgctc tcccgcgaac gcgcccaggc tctcgccgcg cgcgccgagg tggtcggaca
      241 ggaagcgctc cgggccgccg aacagccgga acaggccggg atcgggcagc gcgcgcatca
      301 ccgccgcgtg gagcgccagc agcgcgggca gatcgcgggc gtcgagcgcg cgcaggtggt
      361 ggccgtgcgg caggggtcgg gtcgtcatga ggaagggggc gtcttgcctg cgtcgccgat
      421 cagctcggcg tagttgaaca tctccagcgg cggcagcatg tccaggcggc gcgcgacccg
      481 caggtcgatc tggagcgaga aacgcgtcag ggtctcgacc gggatgccct gcgcgggcgc
      541 ctggcccgac aggatgcgct cggccttgta ggcggcgaac tggccgacgt tgtagtagcg
      601 gctcacgagg cccagcagcg cgcccgaggc catcagctgc tcggtggacg cgaacgccgg
      661 cagccgcgcc tccagcgccg ccggcgccac cacggcgcgc gcctgcgtgc cgaggaagga
      721 atcgggcggc atgtacagcc actgcgcgcc cgcggcctgc agcccgtgca cgatgtccgc
      781 ggcgccgttc gcgccgggct ggccggcggc gtcgacgcgg aaaggccgcg ccagcacctc
      841 gaagccgcgc cgccgcccga gggcctgcag ctcccgcagc acggccgcgg aattgggctc
      901 gttggacgag tacagcatgc ccagccgcgc gaaaggccgg tagctcgcca tcgcctgcac
      961 ctgcgcctcc aggccggcca tgtggctgac gccggtcacc gggcggccgg gcgagcgcag
     1021 gtcgcgcacg atgcccgcgc ccaccggcga cgccaccagg ctgaacacga tggggatgcc
     1081 gcgcaggtgg cggctcgcgt cggcctcgtc ccagcgcccc gccacgccca gcgtgacgcc
     1141 cgtgccccag gtgtagacga ggtcgggccg ctcgcggcgg atctcgtcga ccagcgccgg
     1201 cagccgcgac gggtcgagcg cgatgtcgcg ccaggtgatc tccaccggca ggccgcgggc
     1261 ggcgaagtag tcggcgaagc cgcgttcgac gtcggtgcgg ccgcggtagg tgatcgcgta
     1321 gatgcgccac gggcgcgagc ccgcgcggcc gagcgcaggc gcgagggcga cggccagcag
     1381 ctcgcggcgg cgcatcatcg cgcgaccctg cgcgccagca gcgcgaagca cagcccgcag
     1441 ccgaggacga tcgttgccat cccgacgaag gcggcggccg gaccggcaag caccgccagg
     1501 ccgcccgcga cgagcggtcc gcaggcgttg cccagacgct cgaccaggcg gtaggcgccg
     1561 aacaccgggc cgctgccgtg ggcctcgatc tccgcggcgc agacctgcga cagcaggctg
     1621 ctctgcgccg cgatcgacag cccctgcccc agccccagca gcgcggtgac ggcatagacc
     1681 gacgccacgc cgccggcgcc cagcagcgcg aagccgccga gcgaggacag gcacagcccc
     1741 gcgccgacca ggcgcgcgag cggcacgccg cgctcgaccc agcgcgtggc ctgcggcagc
     1801 accagcacca gcgccacggc gtacagcatg atcgcgcgac cggcgaccga aggcgacgcg
     1861 tcggcgcggg cgacgaacac cggcacgagg tagaagcacg agcccgccag gatcagcttg
     1921 gccggcagcg ccgccaggc
//
LOCUS       DASVLN010000070         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_165155, whole genome shotgun sequence.
ACCESSION   DASVLN010000070 DASVLN010000000
VERSION     DASVLN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165155"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /gene="tmk"
                     /locus_tag="VF453_01550"
     CDS             complement(<1..403)
                     /gene="tmk"
                     /locus_tag="VF453_01550"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7686354.1"
                     /translation="MTPTTANPARRGRFVTFEGIDGAGKSSHVEALAAWARARGAEVV
                     LTREPGGTPLAERLRELFLHEAMDPLGEALLVFAARRDHLMRVIEPALARGALVLCDR
                     FTDATFAYQGGGRGFDREVLSQLERWVQGERQ"
     gene            complement(435..1427)
                     /gene="mltG"
                     /locus_tag="VF453_01555"
     CDS             complement(435..1427)
                     /gene="mltG"
                     /locus_tag="VF453_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7686355.1"
                     /translation="MLKRLVLLLAVLCAAVAGVAGWWLGRPLRLAHDDVEVSIEPGTP
                     VREIVRLWHGAGVETSPELLYEWFRWSGDARRIRAGSYRIDRGATPRSLLDKMVRGDE
                     QLESVHIIDGWTVRQMRAALAAAPDLKHTTAGMSDAQLMAAIGAPGVPAEGRFFPDTY
                     VYSKGVSDLLVLKRAHEALERQLAAAWALRAPDSPLKDADAALTLASVVEKETGVAAD
                     RGKVAGVFANRLRLGMPLQSDPTVIYGLGQSYDGTIHKVDLQTDTPYNSYTRTGLPPT
                     PIALPGRDALRAAVRPEPTKALYFVSRGDGTSVFSETLNAHNQAVNQYQRGSHK"
     gene            1511..>1985
                     /locus_tag="VF453_01560"
     CDS             1511..>1985
                     /locus_tag="VF453_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX7686356.1"
                     /translation="MTASAASTGSAAPSTDALPAGPSAVRLSDWGVIRARGEDAASFL
                     NGQLTQDMASLEPGQARLAGYCTPKGRLLASFVAWRPAADEILLACSADLLATTLKRL
                     SMYVLRAKCKLTDASAELALWGAVIPGAVSADVPAEPWSAAPAGAGTAIRLPDAGG"
BASE COUNT          251 a          725 c          748 g          261 t
ORIGIN      
        1 gctggcgctc gccctgcacc cagcgctcga gctgcgagag cacctcgcgg tcgaagccgc
       61 ggccgccgcc ctggtaggcg aaggtcgcgt cggtgaagcg gtcgcacagc acgagcgcgc
      121 cgcgcgcgag cgcgggctcg atcacgcgca tcaggtggtc gcgccgcgcc gcgaagacca
      181 gcagcgcctc gccgagcgga tccatcgcct cgtgcaggaa cagctcgcgc agccgctcgg
      241 ccagcggcgt gccgcccggc tcgcgcgtga gcacgacctc ggcgccgcgc gcgcgcgccc
      301 aggcggcgag cgcctcgacg tgcgaggact tgccggcacc gtcgatccct tcgaaggtga
      361 cgaagcggcc gcggcgggcg gggttggcgg tggtcggagt caaggcgatc ggggggcggg
      421 cggcgcgcgt caggtcactt gtgcgatccg cgctggtact ggttgacggc ctgattatgg
      481 gcgttgagcg tctcgctgaa gacgctcgtg ccgtcgccgc gcgagacgaa gtacagcgcc
      541 ttggtaggct cggggcgcac cgcggcgcgc agcgcgtcgc gcccgggcag cgcgatcggc
      601 gtcggcggca gcccggtgcg ggtgtagctg ttgtagggcg tgtcggtctg caggtcgacc
      661 ttgtggatcg tgccgtcgta gctctgcccg aggccgtaga tcaccgtcgg gtcggactgc
      721 agcggcatgc cgaggcgcag ccggttggcg aacacgccgg cgaccttgcc gcggtcggcg
      781 gcgacgccgg tctccttctc gacgaccgag gcgagcgtga gcgcggcgtc ggcgtccttc
      841 agcggcgagt ccggcgcacg cagcgcccag gcggcggcga gctggcgctc cagcgcctcg
      901 tgcgcgcgct tgagcacgag gaggtcgctg acgcccttgc tgtagacata ggtgtcgggg
      961 aagaagcgcc cttccgcggg cacgcccggc gcgccgatcg cggccatcag ctgggcgtcg
     1021 ctcatgccgg cggtcgtgtg cttgaggtcg ggcgcggcgg cgagcgcggc gcgcatctgg
     1081 cgcaccgtcc agccgtcgat gatgtggacg gattcgagct gctcgtcgcc gcgcaccatc
     1141 ttgtcgagca gcgacctcgg cgtcgcgccg cggtcgatgc ggtagctgcc ggcgcggatg
     1201 cgacgcgcgt cgcccgacca gcggaaccac tcgtacagca gctcgggcga ggtctcgacg
     1261 ccggcgccgt gccacagccg cacgatctcg cgcacgggcg tgcccggctc gatcgagacc
     1321 tcgacgtcgt cgtgcgcgag gcgcagcggg cggccgagcc accaccctgc gacgccggcg
     1381 acggcggcgc acagcacggc gagcaggagg acaaggcgtt tgagcatgga ggcgaggcgc
     1441 gccccgcagc cagcggccgg gcgagcccct gttcgcgacg ggatccgggg gcgacatgat
     1501 aatcgcgccc atgaccgctt ccgccgcctc caccggctcc gccgccccgt ccaccgacgc
     1561 cctgcccgcc ggcccgtccg ccgtccgcct ctccgactgg ggcgtgatcc gtgcccgcgg
     1621 cgaggacgcc gcgagctttc tcaacggcca gctgacgcag gacatggcca gcctcgagcc
     1681 cggccaggcc cgccttgccg gctactgcac gcccaagggc cggctgctcg cgagcttcgt
     1741 ggcctggcgt cccgcggccg acgagatcct gctcgcctgc agcgccgacc tgctcgcgac
     1801 gaccctcaag cgcctgtcga tgtacgtgct gcgcgccaag tgcaagctga cggatgcgtc
     1861 cgccgagctc gcgctatggg gcgcggtgat acctggtgcc gtatccgccg acgtaccggc
     1921 cgaaccgtgg agcgccgcgc ccgcgggcgc cggcaccgcg atccgcctgc ccgacgccgg
     1981 cggcc
//
LOCUS       DASVLN010000071         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_272826, whole genome shotgun sequence.
ACCESSION   DASVLN010000071 DASVLN010000000
VERSION     DASVLN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272826"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..539)
                     /locus_tag="VF453_01565"
     CDS             complement(90..539)
                     /locus_tag="VF453_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX7686357.1"
                     /translation="MATPRNNPYGAFNYLVSLGTGDESSVIAGFSDVSGLGTEINYSE
                     YRNGNDPVGTVHKIANTFKQDDVTLKRGLIGSLDLFQWIKDVREGADGRRSVTITLLD
                     EARNPVATFRLVNAQPKKWVGPTLAAKGGGEVAMEELHLVHEGVSYE"
     gene            complement(568..>1805)
                     /locus_tag="VF453_01570"
     CDS             complement(568..>1805)
                     /locus_tag="VF453_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEX7686358.1"
                     /translation="ADGRQTFLDSTGAAAAVAAGTLIALVEMKVLVHGPGGRVDAYSQ
                     LALGQEQQRYVGKVLEADDPEDEDAMVWLEWTPDPNGFSPAALAIGLQAADKRLSGGT
                     DGELPGPDALQGDEADPDDVTLKATGLEALGEIDDIAIVALPDAGAMPDDDTIFAAAQ
                     ALVAHAEKCRYRIAVVDAKVNSSMTEIRNFRGQFDTEYGALYHPWIEILDPLYPAVPG
                     VPPRKLLLPPSGFVTGIYARSDIARGVQKAPANEVVNGLTRFEININQARNEVLNPEG
                     VNCLRFFEGRGNRVWGARTMSSDPEWKYVNVRRLFIYMEHSIDKATQWAVFEPNNERL
                     WAGVRRSVEDFLYVLWRDGALLGAKPEQAYFVRCDRTTMTQNDLDNGRMICLVGVAPV
                     RPAEFVIFRIGQWTADSQS"
BASE COUNT          241 a          606 c          654 g          304 t
ORIGIN      
        1 cgcgaagacg ccgggcgagc cgagcggcga gcggacggtg ggcagcatgg cggcgtgggg
       61 cgtcgacgcg cggaagtcgt ccgggcggcc tactcgtagc tcacgccttc gtggacgagg
      121 tgcagctcct ccatcgcgac ctcgcccccg cccttggccg cgagcgtcgg cccgacccac
      181 ttcttgggct gcgcgttgac gagccggaag gtcgcgaccg ggttgcgcgc ctcgtccagc
      241 agcgtgatcg tcacgctgcg gcggccgtcg gcgccctcgc ggacatcctt gatccactgg
      301 aacaggtcca gcgagccgat caggccgcgc ttgagcgtca cgtcgtcctg cttgaacgtg
      361 ttggcgatct tgtggacggt gccgacgggg tcgttgccgt tgcggtactc ggaatagttg
      421 atctcggtgc ccaggcccga gacgtcggag aaaccggcga tcacgctgct ctcgtcgccg
      481 gtgcccagcg agacgaggta gttgaaggcg ccgtaggggt tgttgcgcgg ggtggccatg
      541 gccatgtcct ccgttcgggt tgccggatca ggactgggaa tcggccgtcc actggccgat
      601 gcggaagatc acgaactccg ccggccgcac cggcgccacg ccgacgaggc agatcatccg
      661 gccgttgtcg aggtcgttct gcgtcatcgt cgtgcggtcg cagcgcacga agtaagcctg
      721 ctcgggcttg gcgccgagca gcgcgccgtc gcgccacagc acatacagga agtcctcgac
      781 cgagcgcctg acgccggccc agaggcgctc gttgttcggc tcgaacacgg cccactgggt
      841 ggccttgtcg atcgagtgct ccatgtagat gaacagccgc cgcacgttga cgtacttcca
      901 ctcggggtcg gagctcatcg tgcgtgcgcc ccacacccgg ttgccgcggc cctcgaagaa
      961 tcgcaggcag ttcacgccct cggggttgag cacctcgttg cgcgcctggt tgatgttgat
     1021 ctcgaagcgc gtgaggccgt tgacgacctc gttggcgggc gccttctgca cgccgcgcgc
     1081 gatgtcgctg cgtgcataga tgccggtcac gaagcccgag ggcggcagca gcagcttgcg
     1141 gggcggcacg ccgggcacgg cggggtacag cgggtcgagg atctcgatcc acgggtgata
     1201 cagggccccg tattcggtgt cgaactggcc gcggaagttg cggatctccg tcatcgagct
     1261 gttgaccttg gcgtcgacca ccgcgatgcg gtagcggcac ttctcggcgt gcgccacgag
     1321 cgcctgcgcc gcggcgaaga tcgtgtcgtc gtcgggcatc gcgccggcgt cgggcagggc
     1381 gacgatcgcg atgtcgtcga tctcgccgag cgcttccagg ccggtggcct tgagcgtgac
     1441 gtcgtcgggg tcggcctcgt cgccctgcag cgcgtcgggg ccgggcagtt cgccgtcggt
     1501 gccgccggac agccgcttgt ccgccgcctg caggccgatc gcgagcgccg ccgggctgaa
     1561 gccgttcggg tcgggcgtcc attcgagcca caccatcgcg tcctcgtcct cggggtcgtc
     1621 cgcctcgagc accttgccca cgtagcgctg ctgttcctgc ccgagcgcga gctgggagta
     1681 cgcgtcgacg cggccgcccg ggccgtggac cagcaccttc atctcgacca gcgcgatcag
     1741 tgtgccggcc gcgacggccg cggcggcgcc ggtggagtcg aggaaggtct gccggccgtc
     1801 ggcgt
//
LOCUS       DASVLN010000072         3930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_218220, whole genome shotgun sequence.
ACCESSION   DASVLN010000072 DASVLN010000000
VERSION     DASVLN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218220"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..886
                     /locus_tag="VF453_01575"
     CDS             5..886
                     /locus_tag="VF453_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7686359.1"
                     /translation="MKHRPLKARNFRSAGEDAAKARPPRLYDGPESSYHLAFTDIDFL
                     LTEELRPVRVQLELMKPELVQHKHHIESTIVLFGSARILPPEAAEAALAVVRTTGDAQ
                     AIKRAETGLDMSRYYAEARRFATMVTAGTRHLATPIHVVTGGGPGIMEAGNRGAHETG
                     GKSIGLNIVLPHEQQPNPYITPELCFQFHYFALRKMHFLMRSVALACFPGGFGTLDEL
                     FEVLTLIQTGKSRPRPILLFGSAFWKRLIDFELLVETGMISPGDVNLFKFVETAEEGW
                     AALKGAYGFEGAEDVSV"
     gene            974..1885
                     /gene="rocF"
                     /locus_tag="VF453_01580"
     CDS             974..1885
                     /gene="rocF"
                     /locus_tag="VF453_01580"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEX7686360.1"
                     /translation="MTKVSLIGAPTDIGAGARGASMGPEAMRVAQIQPTLESHGIEVV
                     DRGNLSGPANPWQPPVDGFRHLGEVAAWNRAVHDAVHAELGLGRLPILLGGDHCLGIG
                     SISAVARHCRETGKKMRVLWLDAHADFNTHVLTPSGNIHGMPVACLCGHGPQELIEIG
                     GHVPAINPKWIRQIGIRSVDPGEKKFVHEMGLEVFDMRYIDEMGMRHTMELALATLDD
                     NTHLHVSFDVDFLDPQIAPGVGTTIPGGPTYREAQLCMEMIADTGRLASLDVMELNPA
                     LDVRNQTAVLAVDLIESLFGKSTLMRK"
     gene            2066..3688
                     /locus_tag="VF453_01585"
     CDS             2066..3688
                     /locus_tag="VF453_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7686361.1"
                     /translation="MRTLARTPAAALALLGALALVPSTPALAAALRVASAFDPQTMDP
                     HSLALEYHTRVVFQVYEPLVGHDRNYRLAPVLATRWEQRDPRTWRFHLRPGVRFHDGS
                     PFTADDAVFSIERALAPPSQRQFVLKGVTGVRKVDPLTIDILLAEPDAVLPHKLVLVA
                     MMCKSWAVAHHVERAQDYNARQETFAVRNANGTGAYRLVRYEPDVRTVLEAFPQWWNR
                     AAADNGNVDQASFVTIRSDATRLAALHSGEVDLVLDPPYQDVDRLRRDPALRVTEIGD
                     IATEYLSFDQHSAALPGVPPGPDGKPRNPFRDLRVRQAVYHAVNVDLVIEKVLRGLAI
                     PTGALLAPMIDDEEPSARAPARLRYDPVAARALLREAGYPDGFDVTMECVNVAFREHV
                     CQAIAAMLTQVGIRVSLHTSPSAQFFPMITQGKVSLAEIGFTGNAGDAWQNLNGLVHT
                     WDAKGGGTFNGGRYSNAALDALIDTLRVEPDPRRRHELVERAQAMAQDDVAYVPLYQR
                     KLAWAMQRRVHVVMSPDDTLAVRWTTADRPSP"
BASE COUNT          593 a         1464 c         1337 g          536 t
ORIGIN      
        1 ccggatgaag catcgccccc tgaaagcccg caacttccgc agcgccggcg aggacgccgc
       61 caaggcccgg cccccgcgcc tgtacgacgg ccccgagagc tcgtaccacc tcgccttcac
      121 cgacatcgac ttcctgctga ccgaggagct gcgcccggta cgcgtgcagc tggagctgat
      181 gaagcccgag ctcgtgcagc acaagcacca catcgagtcg acgatcgtgc tgttcggcag
      241 cgcgcgcatc ctgccgccgg aggcggccga ggccgcgctc gccgtggtgc gcacgacggg
      301 cgacgcgcag gcgatcaagc gcgccgagac cggcctcgac atgagccgct actacgccga
      361 ggcgcgccgc ttcgccacca tggtgacggc cggcacccgc cacctcgcga cgccgatcca
      421 cgtcgtcacc ggcggcggcc cgggcatcat ggaggcgggc aaccgcggcg cgcacgagac
      481 cggcggcaag agcatcggcc tgaacatcgt gctgccccac gagcagcagc ccaacccgta
      541 catcacgccg gagctgtgct tccagttcca ctatttcgcg ctgcgcaaga tgcacttcct
      601 gatgcgctcg gtggcgctgg cgtgcttccc cggcggcttc ggcacgctcg acgagctgtt
      661 cgaggtgctc acgctgatcc agaccggcaa gtcgcgcccg cgcccgatcc tgctgttcgg
      721 cagcgcgttc tggaagcggc tgatcgactt cgagctgctc gtcgagaccg gcatgatcag
      781 ccccggcgac gtgaacctgt tcaagttcgt cgagaccgcc gaggaaggct gggccgcatt
      841 gaagggcgcc tacggcttcg agggcgccga ggacgtgagc gtctgatccg ccgatgcggc
      901 cgggtggagg aatgtcgcct gccccgggct cctaccgaat gattcgcacc tgcgcagcac
      961 ggagaatcgc gccatgacca aagtctccct catcggcgca ccgaccgaca tcggtgcagg
     1021 cgcccgcggc gccagcatgg gcccggaagc gatgcgcgtc gcgcagatcc agccgacgct
     1081 ggaatcgcac ggcatcgagg tcgtcgatcg cggcaacctg agcggcccgg ccaacccgtg
     1141 gcaaccgccg gtggacggct tccgtcacct gggcgaggtc gcggcctgga accgcgccgt
     1201 gcacgacgcc gtccacgcgg agctcgggct cggccgcctg cccatcctgc tgggcggcga
     1261 ccactgcctg ggcatcggct cgatcagcgc cgtcgcgcgc cattgccgcg agacgggcaa
     1321 gaagatgcgc gtgctctggc tggacgccca cgccgacttc aacacccacg tcctcacgcc
     1381 cagcggcaac atccacggca tgccggtggc ctgcctgtgc ggccacggcc cgcaggagct
     1441 gatcgagatc ggcggccacg tgcccgccat caacccgaag tggatccgcc agatcgggat
     1501 ccgcagcgtc gacccgggcg agaagaagtt cgtccacgag atgggcctcg aggtgttcga
     1561 catgcgctac atcgacgaga tgggcatgcg ccacacgatg gagctcgcgc tcgccacgct
     1621 ggacgacaac acgcacctgc acgtgagctt cgacgtcgac ttcctcgacc cgcagatcgc
     1681 ccccggcgtc ggcaccacca tccccggcgg tccgacctac cgcgaggcgc agctgtgcat
     1741 ggagatgatc gccgacaccg gccgcctcgc gtcgctcgac gtgatggagc tgaacccggc
     1801 gctggacgtg cgcaaccaga cggccgtgct cgcggtcgac ctgatcgaga gcctgttcgg
     1861 caagagcacg ctgatgcgca agtgactttg gtcgcgcggg acgcgggttt tcgctgaccg
     1921 cgccgatgtc ccgccgacgg cccggcgacg catactcgcc cgggtaacgt ggacgaggag
     1981 cgggcgcatg aggctggcac gcgggatccg tcgcagggcc ggtcgggccg cgcgcgggct
     2041 tgcgcgcatc ctcgctcggg ccatcgtgcg gacgcttgcg cggacgcccg cggccgcgct
     2101 ggcgcttctc ggcgcgctgg ccctggtgcc ctcgacgccg gcgctcgcgg ccgcgctgcg
     2161 cgtcgccagc gcgttcgacc cgcagacgat ggatccgcac agcctcgcgc tggagtacca
     2221 cacgcgggtc gtcttccagg tctacgagcc gctcgtcggc cacgaccgca actaccgcct
     2281 cgcgcccgtg ctggccacgc gctgggagca gcgcgacccg cgcacctggc gcttccacct
     2341 gcggccgggc gtgcgcttcc acgacggcag cccgttcacg gccgacgacg cggtgttctc
     2401 gatcgagcgc gcgctggcgc cgccgtcgca gcgccagttc gtgctcaagg gcgtcacggg
     2461 cgtgcgcaag gtcgacccgc tgacgatcga catcctgctc gccgagcccg atgccgtgct
     2521 gccgcacaag ctcgtgctgg tcgccatgat gtgcaagtcg tgggcggtcg cgcaccacgt
     2581 cgagcgcgcg caggactaca acgcgcgtca ggagaccttc gccgtgcgca acgccaacgg
     2641 caccggcgcc taccgcctcg tgcgctacga gcccgacgtg cgcaccgtgc tcgaggcctt
     2701 cccgcagtgg tggaaccgcg cggcggcgga caacggcaac gtcgaccagg cgagcttcgt
     2761 gacgatccgc tcggacgcga cgcggcttgc ggcgctgcac tcgggcgagg tcgacctcgt
     2821 gctcgacccg ccgtaccagg acgtcgaccg cctgcggcgc gatcccgccc tgcgcgtgac
     2881 cgagatcggc gacatcgcca ccgagtacct cagcttcgac cagcacagcg ccgcgctgcc
     2941 cggcgtcccg cccgggccgg acggcaagcc gcgcaacccc ttccgcgacc tgcgcgtgcg
     3001 ccaggctgtc taccacgcgg tcaacgtcga cctcgtgatc gagaaggtgc tgcgcggcct
     3061 ggccatcccg accggggcgc tgctggcccc catgatcgac gacgaggagc cctccgcgcg
     3121 cgcgcccgcg cggctgcgct acgacccggt cgccgcgcgc gcgctgctgc gcgaggccgg
     3181 ctacccggac ggcttcgacg tgacgatgga atgcgtgaac gtcgcgttcc gcgagcacgt
     3241 ctgccaggcg atcgcggcga tgctgacgca ggtgggcatc cgggtgagcc tgcacacgtc
     3301 gccgagcgcg cagttcttcc ccatgatcac gcagggcaag gtgagcctgg cggagatcgg
     3361 cttcaccggc aacgcggggg acgcctggca gaacctgaac ggcctcgtcc acacctggga
     3421 cgccaagggc ggcggcacct tcaacggcgg gcgctactcg aacgccgcgc tcgacgcgct
     3481 gatcgacacg ctgcgggtgg agcccgaccc gcgccgccgc cacgagctcg tggagcgtgc
     3541 gcaggcgatg gcccaggacg acgtcgccta cgtgccgctg taccagcgca agctggcctg
     3601 ggcgatgcag cgccgggtgc atgtcgtgat gtcaccggac gacacgctcg ccgtgcgttg
     3661 gacgaccgcc gaccggcctt cgccgtgaag cgcgtcacgc ttgtgcgcaa tctcgcgtga
     3721 cagcggtgac cggaggtgcg cgcaggcccc tctcgggaac gcggaatgcc aactcttccg
     3781 cacgctgtca ccgcctttcc gagaacgacg agacgcccgg cccatggaca tgccaaccat
     3841 ccgccgccgc ccgctggacg ccctgctgcg gctcgtggcg ctcgcgccgg cgctggccgc
     3901 ggtcggcgtc gcgctcggcg cggcgctgct
//
LOCUS       DASVLN010000073         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_165242, whole genome shotgun sequence.
ACCESSION   DASVLN010000073 DASVLN010000000
VERSION     DASVLN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165242"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF453_01590"
     CDS             complement(<1..219)
                     /locus_tag="VF453_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="HEX7686362.1"
                     /translation="MTKTYVQIQKQIETLQREADKLKRKEVDDVIAKIKEAISVYELT
                     AADLGLDGKPGRRRAAGGRPAAKSRPARK"
     gene            complement(737..895)
                     /locus_tag="VF453_01595"
     CDS             complement(737..895)
                     /locus_tag="VF453_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX7686363.1"
                     /translation="MTLGTILLIVLVLFLLGAIPAWPYSRGWGYAPSGVLGLLVVILL
                     IMLLTGHL"
     gene            1077..2384
                     /locus_tag="VF453_01600"
     CDS             1077..2384
                     /locus_tag="VF453_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS family transporter"
                     /protein_id="HEX7686364.1"
                     /translation="MDATANVTPGHYSSEEKRRRIFAIMAASSGNLVEWFDFYIYAFT
                     AIYFAPAFFPKSNDTVQLLQAAAVFAIGFLMRPIGGWLFGKIADRRGRKTSLVISVVL
                     MCAGSLLVACLPPYAEIGLAAPALLLLARMVQGLSVGGEYGATATYLSEVALRGQRGF
                     LSSFQYVTLIGGQLLAVLVVFLLQRFLDDAALKAWGWRVPFVIGAITAVVALLLRRTL
                     HETASQAALESREAGSFGYILRNHLGAFLTVVGYTAGGSLIFYTYTTYMQKYLITTAH
                     MPKTTATTISLVTLFAYMCMQPLFGALSDRIGRRHSMMMFGALGALCTVPLMTAIGSA
                     TSPVVAGVLITAALAIVSLYTSVAAIVKAEMFPPEVRALGVGVGYAISNAIFGGSAEY
                     VALQLKDLHMESAFFWYVTAMMVVTFLVSLRLPRVASYLSSDH"
     gene            complement(2424..2723)
                     /locus_tag="VF453_01605"
     CDS             complement(2424..2723)
                     /locus_tag="VF453_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEX7686365.1"
                     /translation="MPSRRHLLALLPLAGAAGLVRAAAPALVDPKDPAAVAQGYVPDA
                     THVDRAKWPKYAPGQSCAGCALYQGAAGSASGPCPIYGGKLVAAKGWCNVFVKKT"
     gene            3016..>3981
                     /locus_tag="VF453_01610"
     CDS             3016..>3981
                     /locus_tag="VF453_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7686366.1"
                     /translation="MTSTLRFLRQPLVASLPLAALVLAGCQTDPNTGAQSIAGVKVSD
                     DPCAKTATVVGGVIGAVTGAVVASQLSKSTDAKVVGGVAGAGIGAAIGYNMDQRRCKL
                     FALAKEHNAEINIAPVVVPKGALPPAVAVAAVDKAGTAESRTTSATPFAPGNDTATSG
                     LSVTLRDSGHQFSPGSDKLTPDADALFRQIADQYSYAKQQAKLGPTSTDDDRAAVETL
                     RTKRILLVGHTDDTGNSTDNANLSERRAEAVARVFQQEGVPATNLYFQGAGETLPIAD
                     NRTEEGRAQNRRVEIVDVTDDASFHKFLATRHQSLQYYRAAGTTET"
BASE COUNT          574 a         1416 c         1388 g          603 t
ORIGIN      
        1 cttgcgcgcc gggcgcgact tggcggccgg acggccgccc gcggcgcggc ggcggccggg
       61 cttgccgtcg aggccgaggt cggccgcggt gagctcgtag acgctgatcg cttccttgat
      121 cttggcgatc acgtcgtcga cttccttgcg cttgagcttg tcggcctcgc gttgcagcgt
      181 ttcgatctgc ttctggatct ggacgtaggt cttggtcatc gtggaggtct ttggaggctt
      241 gttatcggaa ttggtcttgc agttcctccc aaccggttcc gccctccggc agtcgacgat
      301 gtcgctgcca cgatcggaat ccgacggcat tcttcggcgt gccgctggag cgcgaactta
      361 tcacgactcg gccgtcaatt ccgggtttcc tggaaataga tcgcgaaaat cgaatcgcgc
      421 ggccggattc cgaacgaccg gcgcgacgaa ttgaattcga attggcatgc gcgacgcatg
      481 cgcccgatcg cgcgctccgg tcgcgatcga tcgcgaattg ccatcggctt tgccgacgga
      541 ctggcacgtc cgcgccggga ttgcggccgg atgcggacgc cgcgcacgcc gcaattggcc
      601 cggacgacgt ccgcgtgcgg gcgccggccg cgggccggac atgcaagcgg gatcacggca
      661 gccgcgaggg ccgcggggat cccgcatcct ggttggccgg gcgggaccgg cgtggcgccg
      721 gcccgcgtcc gccgcgtcac aagtggccgg tcagcagcat gatcagcagg atcacgacca
      781 gcaggccgag gacgccgctg ggcgcgtagc cccagccgcg gctgtagggc caggccggga
      841 tggcgccgag caggaacagg acgagaacga tcaggagaat ggtgccaagt gtcatgatgg
      901 gttgctcctc gcgggtggtt ggtcgcccgg gccggggagc ccacggcgtg tgtgcctgcc
      961 ttccagcaat tcccggaccc tgcgcccgcc ggatgcgagc tttccctcat ccgcacggat
     1021 ggcccgggca tccccgcgcg gtggagaatg acccgatcac acaggagacc cgccgcatgg
     1081 acgccaccgc gaacgtcacc cccggccact acagctccga ggagaagcgc cggcgcatct
     1141 tcgcgatcat ggcggcctcc tcgggcaacc tggtggagtg gttcgacttc tacatctacg
     1201 cattcacggc gatctatttc gcgccggcgt tcttccccaa gtcgaacgac accgtgcagc
     1261 tgctgcaggc ggcggcggtg ttcgcgatcg gcttcctcat gcggcccatc gggggatggc
     1321 tgttcggcaa gatcgccgac cgccgcggcc gcaagacctc gctggtgatc tcggtggtgc
     1381 tgatgtgcgc gggttcgctg ctcgtcgcct gcctgccgcc gtacgcggag atcggactgg
     1441 ccgcgccggc gctgctgctg ctcgcgcgca tggtgcaggg cctgtcggtc ggcggcgagt
     1501 acggcgccac ggcgacctac ctgagcgagg tggcgctgcg cggccagcgc ggcttcctgt
     1561 cctcgttcca gtacgtcacg ctgatcggcg gccagctgct ggccgtgctc gtggtcttcc
     1621 tgctgcagcg cttcctcgac gacgccgcgc tgaaggcctg gggctggcgc gtgccgttcg
     1681 tgatcggcgc gatcaccgcg gtcgtcgccc tgctgctgcg ccgcacgctg cacgagacgg
     1741 cgtcgcaggc cgcgctcgag agccgcgaag ccggcagctt cggctacatc ctgcgcaacc
     1801 acctcggcgc cttcctcacc gtcgtgggct acacggcggg cggctcgctg atcttctaca
     1861 cgtacacgac ctacatgcag aagtacctga tcacgaccgc gcacatgccc aagacgaccg
     1921 cgacgacgat ctcgctcgtg acgctgttcg cctacatgtg catgcagccg ctgttcggcg
     1981 cgctgtccga ccgcatcggg cgccggcact cgatgatgat gttcggcgcg ctgggcgcgc
     2041 tctgcaccgt gccgctgatg acggccatcg gctccgcgac ctcgccggtc gtcgccggcg
     2101 tgctgatcac ggcggcgctg gcgatcgtca gcctgtacac ctcggtcgcg gcgatcgtga
     2161 aggcggagat gttcccgccc gaggtgcgcg ccctgggcgt cggcgtgggc tacgcgatct
     2221 cgaacgcgat cttcggcggc tcggcggaat acgtcgcgct gcagctgaag gatctccaca
     2281 tggagtcggc cttcttctgg tacgtgacgg cgatgatggt ggtcaccttc ctggtcagcc
     2341 tgcgcctgcc gcgcgtcgcg agctacctga gcagcgacca ttgagccccg gccgcggcgg
     2401 atgcgccgcc gcggccgggt ggttcaggtc ttcttgacga acacgttgca ccagcccttg
     2461 gcggcgacca gcttgccgcc gtagatcggg cacggccccg aggcggagcc ggccgcgccc
     2521 tggtacagcg cacagccggc gcacgactgc ccgggggcgt acttcggcca cttggcgcgg
     2581 tcgacatgcg tggcgtccgg gacgtagccc tgggcgacgg cggcgggatc cttggggtcg
     2641 acgagcgcgg gcgcggcggc gcggacgagg ccggccgcgc ccgccagcgg caggagcgcg
     2701 aggagatggc gacgggaagg catgggctga tcggcaggtt gcggcgacgc ccgattgtgc
     2761 ggccggcacg cgcgttcccg agccccgcac tcgggcatgc cgcgtgctcg gccgcgacgg
     2821 ctgctcgacc gtggcgggat ccccccgatt ggcgattccg accgcccccc ggcccgtgat
     2881 tgaatagcag ccgcgttgcc ggaccgggtg ggcccggcac gcgaggacac ggccgccgcc
     2941 ggagcccggg cgctccgcgg atcccggggg gacggccgca cgacagacga cacgcccacc
     3001 cgcggagccc tcctcatgac ctcgacgctt cgattcctcc gacaaccgct ggtcgccagc
     3061 ctgccgctgg ccgccctcgt gctcgccggc tgccagaccg acccgaacac cggggcgcag
     3121 tccatcgccg gcgtcaaggt gagcgacgac ccgtgcgcaa agacggcgac ggtcgtcggc
     3181 ggcgtgatcg gcgccgtcac gggcgccgtg gtggcgtcgc agctcagcaa gtcgacggac
     3241 gccaaggtgg tcggcggcgt cgcgggcgcg ggcatcggcg cggccatcgg ctacaacatg
     3301 gaccagcggc gctgcaagct cttcgcgctc gccaaggaac acaacgccga gatcaacatc
     3361 gccccggtcg tcgtacccaa gggcgcgctg ccgccggccg tcgcggtggc cgcggtcgac
     3421 aaggccggca ccgccgaatc gcgcaccacc tcggcgacgc cgttcgcccc tggcaacgac
     3481 accgccacca gcgggctgtc ggtcacgctg cgcgactcgg gccaccagtt ctcccccggc
     3541 tcggacaagc tcacgcccga cgccgatgcg ctgttccgcc agatcgccga ccagtactcg
     3601 tacgcgaagc agcaggccaa gctgggaccg acctcgaccg acgacgaccg cgccgccgtc
     3661 gagacgctgc gcaccaagcg catcctgctg gtgggccaca cggacgacac gggcaactcc
     3721 acggacaacg cgaacctctc cgagcgccgc gccgaggccg tcgcccgggt gttccagcag
     3781 gagggcgtgc cggccacgaa cctctacttc cagggcgccg gcgagacgct gccgatcgcc
     3841 gacaaccgca ccgaggaagg ccgggcgcag aaccggcgcg tggagatcgt cgacgtcacc
     3901 gacgacgcgt cgttccacaa gttcctcgcg acgcgccacc agtcgctgca gtactaccgc
     3961 gccgccggca cgacggagac c
//
LOCUS       DASVLN010000074         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_57331, whole genome shotgun sequence.
ACCESSION   DASVLN010000074 DASVLN010000000
VERSION     DASVLN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57331"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..602
                     /locus_tag="VF453_01615"
     CDS             105..602
                     /locus_tag="VF453_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7686367.1"
                     /translation="MTHLAPGDEVFEGGCDCRAVRYRLLARPLFVHCCHCRWCQRETG
                     SAFVINALIESGHLDVTDGEADLVDTPSASGSGQVIARCPRCRIALWSHYAGAGTRLA
                     FVRVGTLDDPDRFPPDIHIHTSSKQPWVLLPPGVPAVPEYYRASEMWPAASLERRRAL
                     FPPQA"
     gene            complement(622..861)
                     /locus_tag="VF453_01620"
     CDS             complement(622..861)
                     /locus_tag="VF453_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686368.1"
                     /translation="MTTIVAGLAVLAVFLVAGYVVQRLSREAPAMPDYCTDDDIRRLA
                     LSGERVVALKWYRQLHRVDVREAVAAVDAMTRKAA"
     gene            complement(1059..2330)
                     /locus_tag="VF453_01625"
     CDS             complement(1059..2330)
                     /locus_tag="VF453_01625"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX7686369.1"
                     /translation="MSNLSYVSPTDDSPWGTYLSQVDRVIPYLGKLSRWAETLKRPKR
                     ALIVDVPIELDNGTVAHFEGFRVQHNLSRGPGKGGVRYHPDVTLEEVMALSAWMTVKN
                     AAVNLPYGGAKGGIRVDPKKLSTKELERMTRRYTSEIGLIIGPQQDIPAPDVNTNGQI
                     MAWMMDTYSMNIGATATGVVTGKPIHLGGSAGRVKATGRGVFVTGREAARRIGLDLNG
                     ARVAVQGFGNVGSSAAELFAQAGAKIVAAQDHTGTIVNPNGFDMADLMPHVHKTGGVA
                     GFAGADSMKNEEFWDVPCEILIPAALEGQVTVDRANRIKAKLVLEGANGPTVPAADDV
                     LKDRGVLVVPDVICNAGGVTVSYFEWVQDFSSFFWSEDEINVRLDKIMVDALKKIWET
                     ADRHKITLRTATFAVACERILIAREERGLYP"
     gene            2569..>2964
                     /locus_tag="VF453_01630"
     CDS             2569..>2964
                     /locus_tag="VF453_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7686370.1"
                     /translation="MMNPNPPDAKPVPAATPGAPSAAPRLAFVGGGNMASAILGGLLA
                     AGHPRERVVVVEPHAPQADLVREKFGVRVDAVGGDALAGSEIVVWAVKPQVFAQAAAP
                     CRPFVGGALQLSVMAGIRSDAIARATGAER"
BASE COUNT          391 a         1044 c         1063 g          466 t
ORIGIN      
        1 ggcgctgctg gccggcgagg cggcggtcgc cggctgagcc cgccgcccgc gcgcgcgggc
       61 gtgcgccaga atgggcgctt ccgacatccc acgggagcgc gaccatgacc cacctggcac
      121 cgggcgacga ggtcttcgag ggcggctgcg attgccgcgc ggtgcgctac cggctgctcg
      181 cgcggccgct gttcgtgcac tgctgccact gccgctggtg ccagcgcgag acgggcagcg
      241 cgttcgtgat caacgcgctg atcgagtcgg ggcacctgga cgtcaccgac ggcgaggcgg
      301 atctcgtcga tacgccgtcg gcgagcggca gcggccaggt catcgcgcgc tgcccgcgct
      361 gccgcatcgc cttgtggagc cactacgcgg gcgcgggcac gcggctggcg ttcgtgcgcg
      421 tgggcaccct cgacgatccc gaccgcttcc cgcccgacat ccacatccac acctcgagca
      481 agcagccgtg ggtgctgctg ccgccgggcg tgccggcggt gcccgagtac taccgcgcgt
      541 ccgagatgtg gccggccgcg tcgctcgagc ggcgccgggc gctgttcccg ccgcaggctt
      601 gagcgggcgg ccgtcgcgcg ttcaggccgc cttgcgggtc atcgcgtcga ccgcggcgac
      661 ggcctcgcgc acgtcgacgc ggtgcagctg gcgataccac ttcagcgcga ccacgcgctc
      721 gcccgacaag gccaggcggc ggatgtcgtc gtcggtgcag tagtcgggca tcgcgggcgc
      781 ctcgcgcgac aggcgctgga cgacgtagcc agccacgagg aacaccgcga gcacggcaag
      841 tcccgcgacg atggtggtca tgatagggtc cccggtatag gtgacgatca cgttgccacg
      901 attgtggaac cggcgtgtga cggccgtcat ggcccctttg ggggaagcgt tgccgacggg
      961 ccacgccgcg cgcgcgcatg aaaaaggccc ccgcaggggc cctcgccggg agcgagccgc
     1021 gcgctgcagc gcagggcttc gccggagcaa gctccgcttc agggatacag gccgcgttcc
     1081 tcgcgcgcga tcaggatccg ctcgcacgcg accgcgaagg tcgcggtgcg cagcgtgatc
     1141 ttgtggcggt cggccgtctc ccagatcttc ttgagcgcgt cgaccatgat cttgtccagg
     1201 cggacgttga tctcgtcctc gctccagaag aagctcgaga agtcctgcac ccactcgaag
     1261 tacgagaccg tcacgccgcc ggcgttgcag atcacgtcgg gcacgacgag cacgccgcga
     1321 tccttcagca cgtcatcggc cgccggcacg gtcgggccgt tcgcgccctc gagcacgagc
     1381 ttggccttga tgcggttggc gcggtcgacc gtgacctggc cttccagcgc ggccgggatc
     1441 aggatctcgc acggcacgtc ccagaactcc tcgttcttca tcgagtccgc gcccgcgaac
     1501 ccggcgacgc cccccgtctt gtggacgtgc ggcatcaggt cggccatgtc gaagccgttc
     1561 gggttgacga tggtgccggt gtggtcctgg gcggccacga tcttggcgcc ggcctgcgcg
     1621 aacagctcgg ccgcggagga gccgacgttg ccgaagccct gcacggccac gcgcgccccg
     1681 ttgaggtcga ggccgatgcg gcgggcggct tcgcggccgg tgacgaacac gccacggccc
     1741 gtggccttga cgcggccggc cgagccgccc aggtggatcg gcttgcccgt gacgacgccg
     1801 gtcgcggtcg cgccgatgtt catcgagtac gtgtccatca tccaggccat gatctggccg
     1861 ttggtgttca cgtccggcgc cgggatgtcc tgctgcgggc cgatgatcag gccgatctcg
     1921 ctcgtgtagc ggcgcgtcat ccgctcgagt tccttcgtcg acagcttctt cggatcgacg
     1981 cggatgccgc ccttggcgcc gccgtagggc aggttcacgg ccgcgttctt gaccgtcatc
     2041 cacgccgaca gcgccatcac ttcttccagc gtgacgtcgg ggtggtagcg cacgccgccc
     2101 ttgccgggac cgcgcgacag gttgtgctgc acgcggaagc cttcgaagtg ggcgaccgtg
     2161 ccgttgtcga gctcgatggg cacgtccacg atcagcgcgc gcttcgggcg cttcagcgtc
     2221 tccgcccagc ggctcagctt gccgaggtac gggatcacgc ggtcgacctg ggacaggtag
     2281 gtgccccacg ggctgtcatc ggtcggggag acgtacgaca ggttgctcat gtcggatttc
     2341 cttggtggat gagtggcggt tcgcgggccg cttcgcctcg tgagcgcggg ccccgcgacg
     2401 tccatggcgc gggtcggatc gatcggatca gcacccccgc gccgggagtg gtgcgcactg
     2461 taggccggac cgcaccgaat tagtcaccga ggcccatgca agttttgcat gatggctgct
     2521 cgtcggtttc atgccggtta cggcaggcgc gggtcctaca ctcgaacgat gatgaacccg
     2581 aaccctcccg acgccaagcc cgtgcccgcc gcgacgcccg gtgcgccctc cgcggcgccg
     2641 cggctcgcct tcgtcggcgg cggcaacatg gccagcgcga tcctcggcgg cctgctcgcg
     2701 gccgggcacc cgcgcgagcg cgtggtggtg gtcgagcccc acgcgccaca agccgacctg
     2761 gtccgtgaga aatttggcgt ccgcgtggac gccgtcggcg gcgacgcgct cgccggttcg
     2821 gagatcgtcg tctgggccgt caagccgcag gtcttcgcgc aggccgcggc gccgtgcagg
     2881 cccttcgtcg gcggcgcgct gcagctgtcg gtgatggcgg gcatccgcag cgacgccatc
     2941 gcgcgcgcca ccggggccga gcgc
//
LOCUS       DASVLN010000075         7274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_273054, whole genome shotgun sequence.
ACCESSION   DASVLN010000075 DASVLN010000000
VERSION     DASVLN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7274
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273054"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VF453_01635"
     CDS             complement(<1..366)
                     /locus_tag="VF453_01635"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX7686371.1"
                     /translation="MSFLAIDIGNTRLKWALYDHAAPGRQPSLHGAVFLETIDQLAET
                     DWAALAPPAQMLGCVVAGEAVRRRVEEQMEMWDVVPRWVVSAAAEAGVVNGYDHPSRL
                     GADRWVALVGARARLQAEAG"
     gene            499..3867
                     /gene="icmF"
                     /locus_tag="VF453_01640"
     CDS             499..3867
                     /gene="icmF"
                     /locus_tag="VF453_01640"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HEX7686372.1"
                     /translation="MTDLSAQYKALAAYRPTHKVRFVTAASLFDGHDAAINIMRRILQ
                     GMGAEVVHLGHNRSVDEVVTAALQEDAQGIAVSSYQGGHNEYFAYMVDLLRQRGGEHI
                     KVFGGGGGVIVPAEIRALADKGVRIFSPEDGQRMGLQGMIGEMVMACDVDLSAYAPTD
                     LAGLAGHTEAAWRRLAQLITAIENKGLPEERVAAVREAAKAVKTPVLGITGTGGAGKS
                     SLTDELIRRIRLDQGDRLHIAVISIDPSRRKSGGALLGDRIRMNAIAPWNQNARRPAG
                     PPQGSAAPSGGSGAAQAASVGARVYMRSLATRDAGSEISEALPDVLAATKLAGFDLII
                     VETSGIGQGDAAIVPLVDVPMYVMTPEFGAASQLEKIDMLDFAEFVAINKFDRKGAAD
                     ALRDVAKQVQRNREAFSTPPDRMPVFGTMASRFNDDGVTALYQALKPRLAELGLRLDE
                     GILPVVDTRHSTHQVPIVPAARTRYLAEIADSVRGYKRNARAQATLAREVQQLRGTAR
                     MLREADADKADAHAAVVALAEQREGRLAPDARKLLAMWPDMRKAYAGDEYVVKIRDKE
                     IRTNLVHTTLAGTKIRKVVLPQFEDHGEILKWLMLDNVPGSFPYTAGVFAFKREGEDP
                     TRMFAGEGDAFRTNRRFKLVSEGMPAKRLSTAFDSVTLYGADPAPRPDIYGKVGNSGV
                     SIATLEDMKVLYSGFDLCSPTTSVSMTINGPAPTILAMFMNTAIDQNLEKFQADNGRE
                     PTADEAAKIRDWVLANVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIAEYFVHHNV
                     RNFYSVSISGYHIAEAGANPISQLAFTLSNGFTFVEAYLARGMHIDDFAPNLSFFFSN
                     GMDPEYTVLGRVARRIWAVAMRDKYGANERSQKLKYHVQTSGRSLHAQEIQFNDIRTT
                     LQALIAIYDNCNSLHTNAFDEAITTPTEDSVRRAMAIQLIINREWGLAKNENPNQGAF
                     VIEELTELVEEAVLAEFEKIAERGGVLGAMETGYQRGKIQEESMHYEMLKHTGEYPII
                     GVNTFRNPHETVPESIELARSTDDEKQSQLRRLAEFHAAHAAESPAMLARLQQAVIEN
                     RNVFEVLMDAVRVCSLGQITGALFEVGGQYRRSM"
     gene            3884..4072
                     /locus_tag="VF453_01645"
     CDS             3884..4072
                     /locus_tag="VF453_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686373.1"
                     /translation="MIKVAIVTTLRAKSGDRAYWLSRPMAERIAAVEKLRRQHAGGGS
                     DVEQGLQRVCRVARLHER"
     gene            4178..4480
                     /locus_tag="VF453_01650"
     CDS             4178..4480
                     /locus_tag="VF453_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686374.1"
                     /translation="MRALSAFGFGGLGLTRDDFMRPDAVVQLGYPPARIDLLTAIDGV
                     SFDACYARRRRLAVDGVELSLIDVDDLRANKRAFGRSKDLADLEALDAAPDNLADD"
     gene            4560..5243
                     /locus_tag="VF453_01655"
     CDS             4560..5243
                     /locus_tag="VF453_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7686375.1"
                     /translation="MNLLLAEDDAILADALTEQLRHAGFETEHAPNGAVAEFLLLKQS
                     WDLCILDLGLPMVDGLTVLQRVRQRKPELPVLVLTALDDLPSRVAGLNAGADDYLTKP
                     FDFPELEARLRALLRRSQSSPVANASMAMGSLVFERDSRRASIDGAPLELSPREWTLL
                     DLLLTRRDRVVTKEEVAQAWSSERAEPGGNNSIEVYIHRVRRKLEGSGLSIRTVRGLG
                     YLLEALRPA"
     gene            5240..6682
                     /locus_tag="VF453_01660"
     CDS             5240..6682
                     /locus_tag="VF453_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7686376.1"
                     /translation="MSKPPPPLLLRAGRLVVGDQSLHKQLLLWLLLPQLVLWLAAAFV
                     TYNVAARYANRAIDASLSQATRALSRQVKPIGNGLFIDFPRAAQDIISADPDDRVYYM
                     VSTPPGQFILGSRKLPPPPLTEPPKLGYPYFYDGVVDKVPLRLAGLYVAYGDDKDRQL
                     MLVQIARGRASRDELARSILGDTVLPLSGLILLMTMIVWAGIRAGLAPLARLRALVED
                     RAPNDLAPLRIDAAPQELRSLARALNDLLAAVHESVAAQRRFVNDAAHQLRTPLAGLK
                     SQTELALGENVDPALAARLQRVHESAVRSAHLVNQLLSLARAEPESALAQQTSRVDLR
                     ELARELTAEWVPKALQAGVDLGLDEAADGAPPVIVVGHALLLREALQNLIDNAIRYAG
                     PSSEVTVSVWRGDDGMGGVQVSDTGPGIPADLHRAVFERFVRATHEGSGCGLGLAIVK
                     SIAERHSGQVTLASLAPHGLQVRIALPLAR"
     gene            7005..>7274
                     /locus_tag="VF453_01665"
     CDS             7005..>7274
                     /locus_tag="VF453_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX7686377.1"
                     /translation="MQGTRASRPALRLVAVAAFALCAANAAHAVDWTGYMRGGPAATT
                     ESGHSRQCYGIGELKYRLGNECDFYGEFQLAQAMKVDGVDVNAVLM"
BASE COUNT         1199 a         2605 c         2449 g         1021 t
ORIGIN      
        1 gcccgcctcg gcctgcagcc gcgcgcgcgc gcccacgagc gcgacccagc gatccgcgcc
       61 caggcgcgag ggatggtcgt agccgttgac gacccccgcc tccgcggccg cggacacgac
      121 ccagcgcggg acgacgtccc acatctccat ctgctcctcg acgcggcggc gcacggcctc
      181 gccggcgacg acgcagccga gcatctgcgc cggcggcgcg agcgcggccc agtcggtctc
      241 ggccagctgg tcgatggtct cgaggaagac ggcgccgtgc aaggacggct gccggccggg
      301 ggcggcgtgg tcgtacagcg cccatttcag gcgcgtgttg ccgatgtcga tggcgaggaa
      361 gctcacagtg cgcgagcgta gcaggccggg cccgggaagc cgaaattgac gtttacgtaa
      421 acgtcaattg tgcccgatac tccaggtttc gcgcgcgcaa cccgccgcct catcgccgcc
      481 cacccgccgg aactcgccat gaccgacctc tccgcacagt acaaggcgct cgctgcctat
      541 cgtcccaccc acaaggtgcg cttcgtcacc gcggccagcc tgttcgacgg ccacgatgcc
      601 gcgatcaaca tcatgcgccg catcctgcag ggcatgggcg ccgaggtggt gcacctgggt
      661 cacaaccgct cggtcgacga ggtcgtcacc gcggcgctgc aggaggacgc gcagggcatc
      721 gccgtcagca gctaccaggg cggccacaac gagtacttcg cctacatggt cgacctgctg
      781 cgccagcgcg gcggcgagca catcaaggtc ttcggcggcg gcggcggcgt gatcgtgccg
      841 gcggagatcc gcgcgctggc cgacaagggc gtgcgcatct tcagccccga ggacgggcag
      901 cgcatgggcc tgcagggcat gatcggcgag atggtgatgg cctgcgacgt cgacctctcg
      961 gcgtacgcgc cgaccgatct cgccggcctg gccggccaca ccgaggccgc gtggcggcgc
     1021 ctggcgcagc tcatcacggc gatcgagaac aaggggctgc ccgaggaacg cgtcgcggcc
     1081 gtgcgcgagg ccgccaaggc ggtgaagacg cccgtcctcg gcatcaccgg caccggcggc
     1141 gcgggcaagt cctcgctgac cgacgaactg atccgccgca tccgcctcga ccagggcgac
     1201 cgcctgcaca tcgccgtcat ctcgatcgac ccgagccggc gcaagagcgg cggcgcgctg
     1261 ctgggcgacc gcatccgcat gaacgcgatc gcgccgtgga accagaacgc gcgccgaccc
     1321 gccgggccgc cccaagggtc ggcagccccc tcggggggca gcggcgccgc gcaagcggcc
     1381 agcgtggggg ctcgtgtcta catgcgctcg ctggccacgc gcgacgccgg cagcgagatc
     1441 agcgaagccc tgcccgacgt gctcgccgcg accaagctcg cgggcttcga cctcatcatc
     1501 gtcgagacct ccggcatcgg ccagggcgac gccgcgatcg tgcccctcgt cgacgtgccg
     1561 atgtacgtga tgacgcccga gttcggcgcc gcgagccagc tcgagaagat cgacatgctg
     1621 gacttcgccg agttcgtcgc gatcaacaag ttcgaccgca agggcgcggc cgacgcgctg
     1681 cgcgacgtcg ccaagcaggt gcagcgcaac cgcgaggcgt tctccacgcc gccggaccgc
     1741 atgcccgtct tcggcacgat ggccagccgg ttcaacgacg acggcgtcac cgcgctgtac
     1801 caggcgctca agccgcgcct ggccgagctc ggcctgcgcc tggacgaggg catcctgccc
     1861 gtggtcgaca cgcgccacag cacgcaccag gtgcccatcg tgccggccgc gcgcacgcgc
     1921 tacctcgccg agatcgccga cagcgtgcgc ggctacaagc gcaacgcgcg cgcgcaagcg
     1981 acgctcgcgc gcgaggtgca acagctgcgc gggacggccc gcatgctgcg cgaggcggac
     2041 gccgacaagg ccgacgcgca cgcggccgtc gtcgcgctcg ccgagcagcg cgaaggccgc
     2101 ctggcgcccg acgcgcgcaa gctgctggcg atgtggcccg acatgcgcaa ggcctatgcg
     2161 ggcgacgagt acgtcgtgaa gatccgcgac aaggagatcc gcaccaacct cgtccacacg
     2221 acgctcgcgg gcacgaagat ccgcaaggtc gtgctgccgc agttcgagga ccacggcgag
     2281 atcctcaaat ggctgatgct ggacaacgtg ccgggcagct tcccgtacac cgcgggcgtg
     2341 ttcgcgttca agcgcgaggg cgaggatccg acccgcatgt tcgccggcga gggcgacgcc
     2401 ttccgcacca accgccgctt caagctcgtc tccgaaggca tgccggccaa gcggctgtcg
     2461 acggcgttcg actcggtcac gctgtacggc gccgaccccg cgccgcgccc ggacatctac
     2521 ggcaaggtgg gcaactccgg ggtctcgatc gcgacgctgg aggacatgaa ggtgctgtac
     2581 tcgggcttcg acctgtgcag cccgacgacc tcggtgtcga tgacgatcaa cggccccgcg
     2641 ccgacgatcc tcgcgatgtt catgaacacc gcgatcgacc agaacctcga gaagttccag
     2701 gcggacaacg gccgcgagcc gaccgccgac gaggccgcga agatcaggga ctgggtgctc
     2761 gccaacgtgc gcggcacggt gcaggccgac atcctgaagg aggaccaggg ccagaacacc
     2821 tgcatcttct cgaccgagtt ctcgctgaag gtgatgggcg acatcgccga gtacttcgtg
     2881 caccacaacg tgcgcaattt ctactcggtg tcgatctcgg gctaccacat cgccgaggcg
     2941 ggcgcgaacc cgatctcgca gctcgcgttc acgctgtcga acggcttcac gttcgtcgag
     3001 gcctacctcg cgcgcggcat gcacatcgac gacttcgcgc ccaacctgtc tttcttcttc
     3061 tcgaacggca tggacccgga gtacacggtg ctgggccgcg tcgcgcgccg catctgggcc
     3121 gtcgcgatgc gcgacaagta cggcgccaac gagcgctcgc agaagctgaa gtaccacgtg
     3181 cagacctcgg gccgctcgct gcacgcgcag gagatccagt tcaacgacat ccgcacgacg
     3241 ctgcaggcgc tgatcgcgat ctacgacaac tgcaactcgc tgcacaccaa cgccttcgac
     3301 gaggcgatca cgacgccgac cgaggactcc gtgcgccgcg cgatggcgat ccagctgatc
     3361 atcaaccgcg aatgggggct ggccaagaac gagaacccga accagggcgc cttcgtcatc
     3421 gaggagctca ccgagctcgt cgaggaggcg gtgctcgccg agttcgagaa gatcgccgag
     3481 cgcggcggcg tcctgggcgc gatggagacc ggctaccagc gcggcaagat ccaggaggag
     3541 tcgatgcact acgagatgct caagcacacg ggcgagtacc cgatcatcgg cgtcaacacc
     3601 ttccgcaatc cgcacgagac cgtgccggaa tcgatcgagc tcgcgcggtc gacggacgac
     3661 gagaagcaga gccaactccg tcgcctggcc gagttccacg ctgcccatgc cgccgaatcg
     3721 cctgcgatgc tggcgcgcct gcagcaggcc gtgatcgaga accgcaacgt gttcgaggtg
     3781 ctgatggacg cggtgcgcgt gtgctcgctg gggcagatca ccggggcgct gttcgaggtg
     3841 ggtggccagt accggcgcag catgtgaggg gtatcatcca ggcatgatca aagtcgccat
     3901 cgtcaccacc ttgcgcgcga agtccggcga ccgtgcgtac tggctgagcc gtccgatggc
     3961 ggaacgcatc gcggcggtgg aaaagctccg tcgccagcac gccggcggcg gttccgatgt
     4021 tgaacaagga cttcaaagag tttgtcgagt tgctcgactc cacgaacgtt gagtacctgc
     4081 tcgtcggcgg ctatgcgctg gcggtgcacg gccatccccg ctacacgggc acatcgactt
     4141 ctgggtaaat ccaacgtcgg aaaacgcgga gcggctcatg cgagcgctgt ccgccttcgg
     4201 cttcggcggc ctgggcctga cccgcgacga tttcatgcga cccgatgcgg ttgtccagct
     4261 gggctatccg cctgcacgca tcgacctgct caccgcgatc gacggcgtgt cgttcgacgc
     4321 ctgctacgcg cggcggcggc gcctcgcggt ggatggcgtc gaactcagcc tgatcgatgt
     4381 cgacgatctg cgagcgaaca agcgagcttt cggccgctcg aaggacctcg cggatctcga
     4441 agcgctcgac gccgcacccg acaacctggc ggacgattga ccgcagcgct gtcgcaggca
     4501 ggcgactgcg ggcgctaccg aaacctcgcc ggccgccgga ggcggtaagc tccgcatcca
     4561 tgaacctcct cctcgccgaa gacgacgcca tcctggcgga cgcgctgacg gagcagctgc
     4621 gccacgccgg cttcgagacg gagcatgcgc ccaacggcgc cgtcgccgaa ttcctgctgc
     4681 tgaagcagtc gtgggacctg tgcatcctcg acctcgggct gcccatggtc gacggcctga
     4741 ccgtgctgca gcgggtgcgg cagaggaagc ccgagctgcc ggtgctggtg ctgacggcgc
     4801 tggacgacct gcccagccgc gtcgccggcc tgaacgccgg cgcggacgac tacctcacca
     4861 agccgttcga cttccccgag ctcgaggcgc ggctgcgcgc gctgctgcgg cgcagccagt
     4921 cctcgccggt ggccaacgcg tcgatggcga tgggctcgct cgtcttcgag cgcgactcgc
     4981 gccgggcgag catcgacggc gcgccgctgg agctgagccc gcgcgaatgg acgttgctcg
     5041 acctgctgct gacgcgccgc gaccgcgtcg tcacgaagga ggaggtcgcc caggcctgga
     5101 gcagcgagcg cgccgagccc ggcggcaaca actccatcga ggtctacatc catcgcgtgc
     5161 ggcgcaagct cgaaggctcc ggcctgagca tccgcacggt gcgcggcctc ggctacctgc
     5221 tcgaggccct gcggccggcg tgagcaagcc cccgccaccg ctgctgctgc gcgccggccg
     5281 gctggtcgtg ggcgaccagt cgctgcacaa gcagctgctg ctgtggctgc tgctgccgca
     5341 gctggtgctg tggctggcag cggccttcgt cacctacaac gtcgccgccc gctatgccaa
     5401 ccgcgcgatc gacgccagcc tgtcgcaggc gacgcgcgcg ctctcacgcc aggtcaagcc
     5461 gatcggcaac gggctgttca tcgacttccc gcgcgcggcg caggacatca tctcggccga
     5521 cccggacgac cgcgtctact acatggtcag cacgccgccg gggcagttca tcctcggcag
     5581 ccgcaagctg ccgccgccgc cgctgaccga gccgcccaag ctgggctacc cgtacttcta
     5641 cgacggcgtc gtcgacaagg tgccgctgcg cctggccggc ctgtacgtgg cctacggcga
     5701 cgacaaggac cgccagctca tgctcgtgca gatcgcccgc ggccgcgcga gccgcgacga
     5761 gctcgcgcgc agcatcctgg gcgacacggt gctgccgctg tcggggctga tcctgctcat
     5821 gacgatgatc gtgtgggccg gcatccgcgc cggcctggcg ccgctcgcgc gactgcgggc
     5881 gctcgtcgag gatcgcgcgc ccaacgacct cgcgccgctg cgcatcgatg cagcgcccca
     5941 ggagctgcgc tcgctcgcgc gcgcgctcaa cgacctgctc gcggcggtgc acgagagcgt
     6001 cgccgcgcag cgtcgcttcg tcaacgacgc cgcgcaccag ctgcgcacgc cgctggccgg
     6061 cttgaagagc cagaccgagc tcgcgctcgg cgagaacgtc gaccccgcgc tcgcggcgcg
     6121 cctgcaacgc gtgcacgaga gcgccgtgcg cagcgcccat ctcgtcaacc agctgctgag
     6181 cctggcgcgc gcggagccgg agtcggccct ggcgcagcag acctcgcgcg tcgacttgcg
     6241 cgagctcgcg cgcgagctca ccgccgagtg ggtgcccaag gcgctgcagg cgggcgtgga
     6301 cctcgggctc gacgaggccg ccgacggcgc gccgccggtg atcgtcgtcg gccacgccct
     6361 gctgctgcgg gaggcgctgc agaacctgat cgacaacgcc atccgctatg ccgggccgtc
     6421 cagcgaggtc acggtgagcg tctggcgcgg cgacgacggc atgggcgggg tgcaggtctc
     6481 ggatacggga cccggtattc ccgccgacct gcatcgcgcg gtgttcgagc gcttcgtgcg
     6541 cgcgacgcac gaaggctccg gttgcggcct ggggctggcc atcgtcaaga gcatcgcgga
     6601 acgccattcg ggccaggtga cgctcgccag cctggcgccc cacgggctgc aggtgcgcat
     6661 cgcgctgccg ctggcccgct gagcggcggc acctcggccg cgaggctgga aaacctcgaa
     6721 atcccttatt catctttcgt caaggtcagc cgaagccaca gtcgggcggc aatcgcggcc
     6781 cgaccgtgcc gaatgccaag aatttctcgc ttcgcccgcg ttgtgcgctg cagcactgca
     6841 agaaatcgtc gagttgcagc gtggcaacaa aaagctcggc actaggcgta aaccctcatt
     6901 aattctgcat taatgagtaa ttaagctttc caccgttggc cggcaaggtg cccggtcagc
     6961 gacacaacaa cgcatccccc cggatgccag caggagacta cgacatgcaa ggaacccgag
     7021 cctcgcgccc ggcactgcgg ctggttgccg tcgccgcttt cgccctttgt gctgccaacg
     7081 ccgcccacgc ggtggactgg accggctaca tgcgcggcgg ccccgccgcc accaccgaat
     7141 ccggccactc gcgccagtgc tacggcatcg gcgagctcaa gtaccgcctg ggcaacgagt
     7201 gcgacttcta cggcgagttc cagctcgcgc aggccatgaa ggtcgacggc gtggacgtca
     7261 acgccgtgct gatg
//
LOCUS       DASVLN010000076         5064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_57425, whole genome shotgun sequence.
ACCESSION   DASVLN010000076 DASVLN010000000
VERSION     DASVLN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57425"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VF453_01670"
     CDS             <1..851
                     /locus_tag="VF453_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686378.1"
                     /translation="QGAIARVPADGGAPQVLVPPATQGLANVQGVLVDAAQGALWACS
                     GDIGFTVVPSTPSALERFDLATGAPRGRFPMPDDGYCNDLAQDASGALYVSDSRHPRI
                     LKLAPGAAALAVWAEDGRLGGPAAFADKHGAYVGLNGIAVGGDGAILASLVAAAPYVL
                     RVPVGADGRAGAPQRLVAPRTLANVDALRAWKPGRLVLFESNAFGAGPYGGRITLARV
                     VGDRLELRPLVAGLNDPSSGVVAGGRVWFIESKYGLLTHRAPADGPVPEGVPFDVESV
                     PLPGDF"
     gene            complement(853..1788)
                     /locus_tag="VF453_01675"
     CDS             complement(853..1788)
                     /locus_tag="VF453_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7686379.1"
                     /translation="MLDALELFLAVASAGTFSQVAKDRAIAVSSVTRRVDALEAEVGA
                     RLFNRSPRRILLTDAGEQFLPRARAILADLAEAKDALAALNADPRGVIVVAAPATFGR
                     IAVVPAVASFLERCPHVEVELHLSDDYLDLSAQRVDVAVRIGALEASELIATRLAPMR
                     LVACASPAYLARHGRPRRPEELLRHACIDIATGAAPHQMWCFDGANQGKPLRVRGRLR
                     VDDRDAMRDAALAGAGIVHIASWLVHEDIAAGRLVELFPGRRPPPARGIERAIHAVRL
                     PGRSNDAKARLFTDHLRETFGDPPYWDRALGRRRA"
     gene            1902..2882
                     /locus_tag="VF453_01680"
     CDS             1902..2882
                     /locus_tag="VF453_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7686380.1"
                     /translation="MLPIRSRFPVLRAAIVTAALASGAAAATSSFAAAPLARSQLPGA
                     YRVQVGDFEVTTLSDGTAMLPVDKLLDQPAARTDAALRKRFLAAPVEMSFNAYLVNTG
                     TRLVLVDTGAGAFFGPTLGRLVQALRAAGVAPEQVDDVLVTHMHPDHVGGLVADGKAV
                     FPNAVVHADKRDADDWLSPATLARATGDAKTFAQDAQAALAPYIAAGRFQAFDGAVEV
                     VPGITAVPAYGHTPGHSCYLVHSGGQRLLVVGDLIHVGAVQFDDPSVAIAFDSDRAAA
                     AATRARVFGKAAKDGTLLAAAHLSFPGLGRLRAAGRSWQWIPADYTTAVK"
     gene            complement(2883..3800)
                     /locus_tag="VF453_01685"
     CDS             complement(2883..3800)
                     /locus_tag="VF453_01685"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7686381.1"
                     /translation="MSSPTPLPTLEFTPLPAPASLTGESPFWHPRERALYWVDIPGRQ
                     LNRFGVDGARHRAWSFESEPSCAAPRPDGGLVIGFRDGIFHFDPATGERRRIVAPPYD
                     PKKLRFNDGKADPQGRFWLGTIHEERLPRGSLYCLTLDALDRIFDDVANSNGLAWSPD
                     GHTMYWTDTKLSTIFAFDFEPQGGTLSRRRVFASFAPKPADGSLEGYGGRPDGGAVDS
                     EGCYWASCFEGQRLVRFAPDGALLQSIPLPVRCPTMPCFGGDDLRTLFVTTSREKRPE
                     AELAAQPWAGRVLQCRVPVPGLPTNFADV"
     gene            4018..>5064
                     /locus_tag="VF453_01690"
     CDS             4018..>5064
                     /locus_tag="VF453_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX7686382.1"
                     /translation="MPRPSPFLRAAAAALAVFAGGQACAAAAPLPALVIDPAQTTVSG
                     LSSGGFMAVQMHVAYSATFHTGAGIVAGGPYYCAQGSLATATGPCMAHDASIPVATLV
                     STTKSWAASGAIDPVANLASSKVYLYSGTQDETVVQAVMNDLKTYYASFVPAANVVYK
                     NTLGSGHAMITDDYGGSCAGTASPYVNDCNFDLAGAILQQLYGPLNPRATGALSGDFV
                     RFDQTAFVSGHGMASTGWLYVPAACSAGQTCRLHVAFHGCLQNAADVGVQFVRDTGYN
                     RWADTNRIVVLYPQTSTSATNSCWDWWGYDSANYAKKSGPQMAAVKAMVDRLASGSAP
                     AAPAPLAAPGGVTAT"
BASE COUNT          666 a         1863 c         1879 g          656 t
ORIGIN      
        1 gccagggcgc gatcgcgcgc gtgccggcgg acggcggcgc gccgcaggtg ctggtgccgc
       61 cggccacgca agggctcgcc aacgtgcagg gcgtgctggt cgatgccgcg cagggcgcgc
      121 tgtgggcctg ctcgggcgac atcggcttca cggtcgtgcc gtcgacgccc agcgcgctcg
      181 agcgcttcga cctggccacc ggcgcgcccc ggggccgctt cccgatgccc gacgacggct
      241 actgcaacga cctcgcgcag gacgcctcgg gcgcgctgta cgtgagcgat tcgcgccatc
      301 cgcgcatcct gaagctcgca cccggcgccg ccgcgcttgc cgtgtgggcc gaggacgggc
      361 ggctcggcgg gccggcggcc ttcgccgaca agcacggggc ctacgtcggc ctcaacggca
      421 tcgcggtcgg cggcgacggc gcgatcctcg cgagcctggt cgctgccgcg ccatatgtgc
      481 tgcgcgtccc ggtcggggcg gacggccgcg ccggggcgcc gcagcgcctg gtcgcgccgc
      541 gcacgctggc caacgtcgac gcgctgcggg cgtggaagcc gggccggctg gtgttgttcg
      601 agagcaatgc gttcggcgcc gggccgtacg gcgggcgcat cacgctcgcg cgcgtggtcg
      661 gcgaccggct cgagctgcgg ccgctcgtgg cggggctcaa cgacccgtcg tcgggcgtcg
      721 tggccggcgg ccgcgtctgg ttcatcgagt cgaagtacgg cctgctcacg caccgcgcgc
      781 ccgccgacgg gccggtgccc gagggcgtgc cgttcgacgt cgagtcggtt ccgctgcccg
      841 gcgacttctg actcaagccc gccgccggcc cagggcccgg tcccagtacg gcgggtcgcc
      901 gaaggtctcg cgcaggtggt cggtgaacaa ccgcgccttg gcatcgttcg agcgcccggg
      961 caggcgcacc gcgtggatgg cgcgctcgat gcccctggca ggcggcggac gccggccggg
     1021 gaacagctcg acgagccggc cggcggcgat gtcctcgtgc accagccagc tcgcgatgtg
     1081 cacgatgccg gcgcccgcga gtgccgcgtc gcgcatcgcg tcgcggtcgt cgacgcgcag
     1141 ccggccgcgc acgcgcagcg gcttgccctg gttggcgccg tcgaagcacc acatctggtg
     1201 cggcgcggcg ccggtggcga tgtcgatgca cgcgtggcgc agcagctcct cgggccggcg
     1261 cggacgcccg tggcgcgcga ggtaggccgg gctcgcgcag gcgacgagcc gcatcggcgc
     1321 gagccgcgtc gcgatcagct cgctcgcctc caacgcgccg atgcgcacgg cgacgtcgac
     1381 acgctgcgcc gacaggtcga ggtagtcgtc gctcaggtgc agctcgacct ccacgtgcgg
     1441 gcagcgctcg aggaagcttg cgaccgccgg cacgacggcg atgcggccga aggtcgccgg
     1501 cgcggccacg acgatgacgc cgcgcgggtc ggcgttgagc gcggcgagcg cgtccttggc
     1561 ctcggccagg tcggcgagga tggcgcgggc gcgcggcagg aactgctcgc cggcgtcggt
     1621 cagcaggatg cgccgcgggc tgcggttgaa caggcgcgcg ccgacctcgg cctcgagcgc
     1681 gtccacgcgc cgcgtgacgg aggagacggc gatggcgcga tccttggcca cctgcgagaa
     1741 ggtgccggcc gaggcgacgg cgagaaagag ttcgagggcg tcgagcatgt tgcggacggc
     1801 gcaatgcaaa tttgcgatgg cgatgattgt tccgcggatc gcaaagccga acaatgtctc
     1861 ccgacgaccc gccgggtcgt gtcacaacga gagaaccgcc gatgcttccg atccgttccc
     1921 gctttcccgt cctgcgcgcc gccatcgtca ccgccgcgct cgcgtccggc gccgccgccg
     1981 ccacctcctc attcgccgcg gcgccgctgg cgcgatccca gctgcccggc gcctaccgcg
     2041 tgcaggtcgg cgacttcgag gtcaccacgc tgagcgacgg cacggcgatg ctgcccgtcg
     2101 acaagctcct cgaccagccg gccgcgcgca ccgacgccgc actgaggaag cgcttcctcg
     2161 ccgcgccggt ggagatgtcg ttcaacgcct acctggtcaa caccggcacg cggctggtgc
     2221 tggtggacac cggtgccggc gcgttcttcg gcccgacgct gggccgcctc gtgcaggcct
     2281 tgcgcgccgc gggcgtggcg ccggagcagg tcgacgacgt gctcgtcacc cacatgcacc
     2341 ccgaccacgt cggcggcctg gtggccgacg gcaaggcggt gttcccgaac gcggtcgtcc
     2401 acgcggacaa gcgcgacgcc gacgactggc tctcccccgc gacgctcgcg cgcgccaccg
     2461 gcgacgcgaa gaccttcgcg caggacgccc aggccgcgct ggcgccctac atcgcggcgg
     2521 gccgcttcca ggcgttcgac ggcgccgtcg aggtcgtgcc gggcatcacc gcggtgcccg
     2581 cctacggcca cacgccgggc cactcgtgct acctggtgca cagcggcggg cagcggctgc
     2641 tggtggtcgg cgacctgatc cacgtcggcg cggtgcagtt cgacgacccg tccgtggcca
     2701 tcgccttcga ctccgaccgc gcggccgccg ccgcgacgcg cgcgcgggtg ttcggcaagg
     2761 ccgcgaagga cggcacgctg ctcgccgcgg cgcatctgtc gttcccgggg ctgggccggc
     2821 tgcgcgcggc ggggcggtcg tggcagtgga tccccgcgga ctacaccacc gcggtcaagt
     2881 gatcagacgt ccgcgaagtt cgtcggcagg ccgggcacgg ggacccggca ctgcagcacg
     2941 cgcccggccc acggctgggc ggcgagctcg gcttccggac gcttctcgcg cgaggtcgtg
     3001 acgaagagcg tgcgcaggtc gtcgccgccg aagcagggca tcgttgggca gcgcaccggc
     3061 agcgggatcg attgcagcag cgcgccgtcg ggcgcgaagc gcacaaggcg ctggccctcg
     3121 aagcaggagg cccagtagca gccctcgctg tccacggcgc ccccgtccgg ccggccgcca
     3181 tagccttcga gcgagccgtc cgccggcttg ggcgcgaagc tcgcgaacac gcgccggcgc
     3241 gacagcgtcc cgccctgcgg ctcgaagtcg aacgcgaaga tcgtcgacag cttggtgtcc
     3301 gtccagtaca tcgtgtggcc gtccgggctc caggcgaggc cgttgctgtt ggcgacgtcg
     3361 tcgaagatgc ggtcgagcgc gtccagcgtc aggcagtaca gcgagccgcg cggcaggcgc
     3421 tcctcgtgga tcgtgccgag ccagaagcgg ccctgaggat cggccttgcc gtcgttgaag
     3481 cgcagcttct tcgggtcgta gggcggggcg acgatgcgcc ggcgctcccc cgtcgcggga
     3541 tcgaagtgga agatgccgtc gcgaaagccg atcacgaggc cgccgtcggg ccgcggcgcg
     3601 gcgcaggacg gctccgactc gaacgaccag gcgcgatgcc gcgcgccgtc gacgccgaag
     3661 cgattgagct gccggcccgg gatgtcgacc cagtacagcg cgcgctcgcg cggatgccag
     3721 aagggcgact cgccggtcag cgacgcgggc gcgggcagcg gcgtgaactc gagcgtgggc
     3781 aggggcgtgg ggctggacat gacgggcgag gcgggaggaa ggccgggggc gatgccgcat
     3841 cctagcggcg taccgcgatg aaaccgcgcc ggcggattgt cggcttttgg cactctcgcc
     3901 gtgcggctta cgcgatgttg cgatggccgg gcaaacccgg gaccggcccc cgcgcgcgcg
     3961 tcgcagaatc gcccgtcggg cgcggccgcc gggcgcgccg tccaaggagg cccgaccatg
     4021 ccacgaccct cgcccttcct gcgtgccgcc gccgcggcgc tcgccgtctt cgccggcgga
     4081 caagcctgcg cggccgccgc gccgctgccg gcgctcgtga tcgaccccgc gcagacgacc
     4141 gtgtcgggcc tgtcctcggg cggcttcatg gccgtgcaga tgcacgtcgc ctactcggcc
     4201 accttccata ccggcgcggg catcgtggcc ggcggcccgt actactgcgc ccagggctcg
     4261 ctggccaccg ccaccggccc gtgcatggcg cacgacgcct cgatcccggt cgcgaccctg
     4321 gtgtcgacga cgaagagctg ggccgccagc ggcgcgatcg acccggtcgc caacctcgcg
     4381 tcgtccaagg tgtacctgta ctcgggcacg caggacgaga cggtcgtgca ggcggtgatg
     4441 aacgacctga agacctacta cgcgagcttc gtgccggcag ccaacgtcgt ctacaagaac
     4501 acgctcggct ccggccacgc gatgatcacc gacgactacg gcggcagttg cgccggcacc
     4561 gcgtcgccgt acgtcaacga ctgcaacttc gacctcgccg gcgcgatcct gcagcagctc
     4621 tacggcccgc tgaacccgcg cgcgacgggc gcgctctcgg gcgacttcgt gcggttcgac
     4681 cagaccgcct tcgtcagcgg ccacggcatg gcctcgaccg gctggctcta cgtgccggcc
     4741 gcctgctccg cggggcagac gtgccggctg cacgtggcct tccacggctg cctgcagaac
     4801 gcggccgacg tcggcgtgca gttcgtgcgc gacaccggct acaaccgctg ggccgacacc
     4861 aaccgcatcg tcgtgctgta tccgcagacc agcacgtcgg ccaccaacag ctgctgggac
     4921 tggtggggct acgacagcgc gaactacgcg aagaagagcg gcccgcagat ggccgctgtc
     4981 aaggcgatgg tcgaccgcct ggccagcggc agcgcccccg ccgcgccggc gccgctggcc
     5041 gcgccgggcg gggtcacggc cacg
//
LOCUS       DASVLN010000077         8605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_165346, whole genome shotgun sequence.
ACCESSION   DASVLN010000077 DASVLN010000000
VERSION     DASVLN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165346"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VF453_01695"
     CDS             <1..1198
                     /locus_tag="VF453_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7686383.1"
                     /translation="RAAAPAGARRPGLDAHQQLSRRGRLIAETGLRWPRVTGPALPFR
                     PGRPALEEFPFDAWQRLERRTTARARIEAAGYADPAGHPALRAAIAQWLFVSRGVRCA
                     PGQVLITAGAQQGLDLVTRLLLDVGDQALIEDPGYGGTRAVLAAQGAIARPVRIDAEG
                     LDIAHGAAQWPQARLAVVTPTHQFPLGVCMSLERRRALIDWARTSGGWIVEDDYDGEF
                     QYGPHRSPALCSMAHDARVIYAGTFSKSLGGGLRIGFLVLPDALVDAFSRARALSDRQ
                     SPGALQEVLARFIADGYLTSHLHRMRELYARRQAFLIDALARATHGAITLAPSRQGMH
                     LAFEIPSSQRDTALSQRAAEGGLQLAPISAYCVEARRRGWLFGYAGFDEATLQRAARS
                     LGRLMR"
     gene            complement(1203..2621)
                     /gene="dnaB"
                     /locus_tag="VF453_01700"
     CDS             complement(1203..2621)
                     /gene="dnaB"
                     /locus_tag="VF453_01700"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX7686384.1"
                     /translation="MTTVFDSALPSRGESRDDEVARLRVPPQSIEAEQSVLGGLLLDN
                     GAWDRAADIVTDSDFYRYEHKLIFAAIGALIGANRPADVITVFEQLQTSGKADDAGGL
                     PYLNQLAQSVPSAANMRRYAEIVRERAVLRKLIATSDEIATNAFNPQGRQVSQILDEA
                     ETKIMKIGEETSRGKQGFFAMNDLAVQLIDRVTELAENGAEDVTGVRTGFYDLDKNTA
                     GLQPGDLIILAARPSMGKTAFALNIAENVSVNEGLPVVVFSMEMGAAQLALRMVGSQG
                     RIDQQHLRTGRLDNDEWGRLTEAVERLSKSSVFIDETPALTGPELRARARRQARTCGK
                     LGLIVIDYLQLMSGSGGDGENRATEIGEISRGLKGLAKELQCPVIALSQLNRSVETRT
                     DKRPMMSDLRESGAIEQDADVIMFIYRDEYYNRETTKEPGIAEIIIAKQRNGPVGTVK
                     LTFLRQWTKFDNLAPDYQSPEY"
     gene            complement(2837..3289)
                     /gene="rplI"
                     /locus_tag="VF453_01705"
     CDS             complement(2837..3289)
                     /gene="rplI"
                     /locus_tag="VF453_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX7686385.1"
                     /translation="MQIILLEKVANLGNLGDVVKVKDGYARNFLIPTRLARRATESAI
                     KEFEAKRAELEKAAAEKLTAAQGVGEKLGGKTVRISQKAGVDGRLFGSVTNADIAEAL
                     GQNGFQVVKAQVRLPNGPLKTVGEHPVSVALHTDVVVDVTVQVVAEQD"
     gene            complement(3303..3587)
                     /gene="rpsR"
                     /locus_tag="VF453_01710"
     CDS             complement(3303..3587)
                     /gene="rpsR"
                     /locus_tag="VF453_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX7686386.1"
                     /translation="MPPPKSRFSKDKRPKRNQQSLLFRRKRFCRFTVAKVEEIDYKDV
                     DTLRDFIGENGKITPARLTGTRAFYQRQLTVAIKRARFLALLPYTDQHKV"
     gene            complement(3641..3925)
                     /gene="priB"
                     /locus_tag="VF453_01715"
     CDS             complement(3641..3925)
                     /gene="priB"
                     /locus_tag="VF453_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEX7686387.1"
                     /translation="MNRLVLAAELVERSATRYTPAGLPALDLSLKHDSEVVEDKHPRK
                     LSLTMRAVAIGEITRRVSTLPLGQVATWIGFLAPSRNARGLVFHVTEVEA"
     gene            complement(3978..4340)
                     /gene="rpsF"
                     /locus_tag="VF453_01720"
     CDS             complement(3978..4340)
                     /gene="rpsF"
                     /locus_tag="VF453_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX7686388.1"
                     /translation="MRHYEIIILIHPDQSEQVPAMLERYKALVTNSGGKVTRLEDWGR
                     RQLAYPIQKLAKAHYICLNIECGKEALVELETGFRFNDAVLRHLTVLRPKADTNPSVM
                     MKAVEKEEARKSAQESAA"
     gene            complement(4545..6956)
                     /gene="ppsA"
                     /locus_tag="VF453_01725"
     CDS             complement(4545..6956)
                     /gene="ppsA"
                     /locus_tag="VF453_01725"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX7686389.1"
                     /translation="MSQPNLATALVVPFEQLRMTDVEAVGGKNASLGEMISQLAASGV
                     RVPGGFATTAHAFREFLRHGGLTERIARRLKSLDTDDVRALAEAGAEIRKLIEDAPFP
                     PELEKAVREEFARLSASDPNASFAVRSSATAEDMPDASFAGQQETFLNVVGIDGVLHK
                     MKEVFASLYNDRAISYRVHKGYADSDVALSAGVQRMVRSDLGAAGVMFTLDTESGFTD
                     VVFITSSYGLGETVVQGAVNPDEFYVHKPALAAGKFPIIRRSLGSKLIRMEFASAEEK
                     ARDGRLVRTAETPPELRNRYSLTDADVVELAKYATIIEKHYGRPMDIEWGKDGGDGLL
                     YILQARPETVKSNAAGQAEHRYKLKGSGTVLTEGRAIGQKIGTGTVRVCRSISEMDQV
                     QNGDVLVADMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATDVL
                     ADGALVTVVCSEGDTGFVYDGLLETEVTEVTRGEMPYTPIKVMMNVGNPQLAFNFSQL
                     PNSGVGLARLEFIINNNIGVHPKAILDYPNIDADLKKAVESVARGHASPRAFYVDKLA
                     EGIATIAAAFWPKPVIVRLSDFKSNEYKKLIGGSRYEPDEENPMLGFRGASRYISGEF
                     QDAFAMECEALKRVRIDMGLSNVEIMVPFVRTVRQAQRVVEMLAERGLKRGANGGNDG
                     LRLIMMCEVPSNAILADQFLEHFDGMSIGSNDLTQLTLGLDRDSGLELLAEDFDERDP
                     AVKAMISRAITACRATGKYVGICGQGPSDHPDFAEWLAQEGIVSISLNPDSVVDTWTR
                     LAAVK"
     gene            7156..7980
                     /locus_tag="VF453_01730"
     CDS             7156..7980
                     /locus_tag="VF453_01730"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX7686390.1"
                     /translation="MPNRTVFFISDGTGITAETFGNSILAQFPGRPRKVRRPFIDTVD
                     KAAKVVEEINHTFVAEGKKPIVFITMASEDVRQALTGPECSGVIMDMFRTFVEPLEVE
                     FGMKSNHRVGRFSDAAKSEEYNERIEAINFSLAHDDGQSSRNLAQADVILVGVSRCGK
                     TPTTLYLAMQHGIKAANYPLIPEDFERGQFPSSLAPFKAKCFGLTIDPERLTQIRNER
                     RPSSKYAALENCRYEVREAESMMKREGISWLSSTHKSIEEIAATILRDIRPDKLIY"
     gene            complement(7974..>8605)
                     /locus_tag="VF453_01735"
     CDS             complement(7974..>8605)
                     /locus_tag="VF453_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7686391.1"
                     /translation="LRAIVTASGWARPELAALARTAPEVVCVPDALMRELSGLESPAP
                     LGFLIDAGRDATLRPGVRTVVLDRLQDPGNAGSLLRTASAMGFAQVVALKGTVALWSP
                     KVLRAGMGAHFGLSLVEGAGLDELRALGVPMVGTHLRDAGWLHEAALPDPLAWVFGHE
                     GQGMSDAVAAACDLRVRIAQPGGEESLNVAAAAAICLHESARRLVARGQ"
BASE COUNT         1236 a         2960 c         3012 g         1397 t
ORIGIN      
        1 ccgcgccgcc gcgcccgcgg gcgcgcggcg gccgggcctg gacgcgcacc agcagctctc
       61 gcgtcgcgga cggctgatcg ccgagaccgg gctgcgctgg ccgcgcgtca cgggcccggc
      121 cctgccgttc cggcccgggc gaccggcgct cgaggagttc ccgttcgacg cttggcagcg
      181 cctcgagcgc cgcaccaccg cacgcgcccg catcgaagcc gcgggctacg ccgatcccgc
      241 gggccatccc gcgctgcggg ccgcgatcgc gcaatggctg ttcgtctcgc gcggcgtgcg
      301 ctgcgcgccg ggccaggtgc tgatcaccgc cggcgcgcag cagggcctgg acctggtgac
      361 acggctgctg ctggacgtcg gcgaccaggc actgatcgag gatcccggct acggcggcac
      421 gcgggcggtg ctcgcggccc agggcgcgat cgcacggccg gtgcgcatcg acgcggaggg
      481 cctcgacatc gcgcacgggg ctgcccaatg gccgcaggcg cggctcgcgg tggtcacgcc
      541 gacgcaccag ttcccgctcg gcgtgtgcat gagcctcgag cgccggcgcg ccctcatcga
      601 ctgggcgcgc acgagcggcg gctggatcgt cgaggacgac tacgacggcg agttccagta
      661 cggcccgcac cgcagcccgg ccctgtgcag catggcccac gacgcgcgcg tgatctacgc
      721 cggcacgttc tcgaagtcgc tgggcggcgg cctgcgcatc ggcttcctgg tgctgccgga
      781 cgcgctcgtg gacgccttct cgcgcgcacg cgcgctgagc gaccgccagt cccccggcgc
      841 gctgcaggag gtgctcgcgc gcttcatcgc cgacggctac ctcacgagcc acttgcaccg
      901 catgcgcgag ctctacgcgc ggcgccaggc cttcctcatc gacgccctcg cccgggcgac
      961 ccacggcgcg atcacgctcg cgcccagccg ccagggcatg cacctcgcct tcgagatccc
     1021 gtcctcgcag cgcgacacgg cgctgagcca gcgtgccgcc gaggggggcc tgcagctggc
     1081 gccgatctcg gcctactgcg tcgaggcgag acggcgcggc tggctgttcg gctacgcggg
     1141 cttcgacgag gcgacgctgc agcgcgccgc gcgctcgctg ggccgcctga tgcgctgagt
     1201 cgtcagtact cggggctctg gtagtccggc gcgaggttgt cgaacttcgt ccactggcgc
     1261 aggaacgtga gcttcaccgt gccgaccggg ccgttacgct gcttggcgat gatgatctcc
     1321 gcgatgccag gctccttggt cgtctcgcgg ttgtagtact cgtcgcggta gatgaacatg
     1381 atcacgtccg cgtcctgctc gatggcgccc gactcgcgca ggtcggacat catcggccgc
     1441 ttgtcggtgc gcgtctcgac cgagcggttc agctgcgaca gcgcgatcac ggggcactgc
     1501 agctccttgg ccaggccctt gaggccgcgc gagatctcgc cgatctcggt cgcgcggttc
     1561 tcgccgtcgc cgccggagcc gctcatcagc tgcaggtagt cgatcacgat cagccccagc
     1621 ttgccgcagg tgcgcgcctg gcggcgcgca cgggcgcgca gctcggggcc ggtcagcgcg
     1681 ggcgtctcgt cgatgaacac cgaggacttg gacagccgct ccacggcctc ggtgagccgc
     1741 ccccactcgt cgttgtccag ccggccggtg cgcaggtgct gctggtcgat gcggccctgg
     1801 gagccgacca tccgcagcgc cagctgcgcc gcgcccattt ccatcgagaa cacgacgacc
     1861 ggcaggcctt cgttgacgga gacgttctcc gcgatgttca gcgcgaacgc ggtcttgccc
     1921 atggacggcc gcgccgccag gatgatgagg tcgcccggct gcaggccggc ggtgttcttg
     1981 tcgaggtcgt agaagccggt gcgcacgccg gtgacgtcct cggcgccgtt ctcggcgagc
     2041 tcggtcacgc ggtcgatcag ttgcaccgcg aggtcgttca tcgcgaagaa gccctgcttt
     2101 ccgcgcgagg tctcctcgcc gatcttcatg atcttggtct cggcctcgtc gaggatctgc
     2161 gagacctggc ggccctgcgg gttgaacgcg ttcgtcgcga tctcgtcgct ggtggcgatc
     2221 agcttgcgca gcacggcacg ctcgcggacg atctccgcgt agcgacgcat gttggccgcg
     2281 ctcggcacgc tctgcgcaag ctggttcagg tacggcaggc cgccggcgtc gtcggccttg
     2341 cccgaggtct gcagctgctc gaagacggtg atgacgtcgg ccgggcggtt cgcgccgatc
     2401 agcgccccga tcgccgcgaa gatcagcttg tgctcgtagc ggtagaagtc gctgtcggtg
     2461 acgatgtccg cggcccggtc ccatgcgccg ttgtccagca gcaggccgcc gagcacgctc
     2521 tgctcggcct cgatcgactg cggcggcacc cgcaggcgcg cgacctcgtc gtcacgggac
     2581 tcgcctcggc tgggcaatgc ggaatcgaag acggtggtca tgaagggacg attcttgcgg
     2641 gcgccgccgg gcttgcgaag tggacaagcc tgtggacagc gcgtggacga ccggtggatc
     2701 acgcaggcct gtgggcaagc ctgtggacac cccggggatg tcctgggggc aggacgggaa
     2761 aaccgccccc ggaaatgaaa aagggccgac gcgcgtcgac cctttcgctc gaagcgaccc
     2821 ggaggtcgcg ctcgcctcag tcttgctcgg cgacgacctg caccgtcacg tcgacgacga
     2881 cgtcggtgtg cagcgcgacg gagaccgggt gttcgccgac ggtcttcagc gggccgttgg
     2941 gcaggcggac ctgcgccttg acgacctgga agccgttctg gcccagggcc tcggcgatgt
     3001 cggcgttggt gaccgaaccg aagaggcggc cgtcgacgcc ggccttctgc gagatgcgca
     3061 cggtcttgcc gcccagcttc tcgccgacgc cctgcgcggc ggtcagcttc tcggcggcgg
     3121 ccttctcgag ctcggcgcgc ttggcttcga attccttgat cgcggactcg gtggcgcggc
     3181 gcgccaggcg ggtcgggatc aggaagttgc gcgcgtagcc gtccttgacc ttgacgacgt
     3241 cgcccaggtt accgaggttg gcgacctttt ccagcaggat gatttgcatg ggtcgtgact
     3301 cctcagacct tgtgttgatc ggtgtacggc agcagggcca ggaagcgggc gcgcttgatg
     3361 gcgaccgtca gctggcgctg gtagaacgcg cgcgtgccgg tcaggcgcgc cggcgtgatc
     3421 ttgccgttct cgccgatgaa gtcgcgcagc gtgtcgacgt ccttgtagtc gatctcctcg
     3481 accttggcga cggtgaagcg gcagaagcgc ttgcggcgga acagcagcga ctgctggttg
     3541 cgcttcgggc gcttgtcctt ggaaaaacga gacttgggcg ggggcatggt gatacctctt
     3601 gcgaaatggg ttccggatgg atgcttgcgg acgcggggcg tcaggcttcg acctcggtca
     3661 cgtggaagac gagcccgcgc gcattgcgcg agggagcgag gaatccgatc caggtggcga
     3721 cctgccccag ggggagggtc gatacccgtc gggtgatctc gccgatcgcc accgctcgca
     3781 tcgtcaggct cagcttgcgg ggatgcttgt cttccaccac ctcggaatcg tgcttcaggc
     3841 tcaggtcgag cgccggaagc ccggccgggg tgtaccgggt cgcgctgcgt tcgaccagct
     3901 cggcggcgag aaccaggcgg ttcatcgctg cggcgccttc atgcgaaggc tgcgagcggc
     3961 ggacgcggcg acgcccttca ggccgcgctc tcctgggccg acttgcgcgc ttcttccttc
     4021 tcgacggcct tcatcatgac cgacgggttg gtgtccgcct tcgggcgcag cacggtgagg
     4081 tggcgcagga cggcgtcgtt gaagcggaag cccgtctcga gctcgaccag ggcttccttg
     4141 ccgcactcga tgttgaggca gatgtagtgg gccttggcaa gcttctggat cgggtaggcc
     4201 agctggcggc ggccccagtc ttccaggcgg gtcaccttgc caccggagtt ggtgacgagg
     4261 gccttgtagc gttccagcat ggccggaacc tgctcactct gatccgggtg gatcaggatg
     4321 atgatctcgt aatgacgcat ggacactcct tgtggatctt cgactcgcgt cgaatggcag
     4381 ccgccccgaa agcgtcgact tctgcgctct caacgagcgc ttcgggtgcg gcaaggaacc
     4441 ggggatcata gcacaaaagg ctccgcgcgg gagccttttg caggaaagga cggccgcgcg
     4501 ccgcgctggc gggatgggcc cgcgcggccg tgcgccatcc ggccttactt gacggccgcc
     4561 aggcgcgtcc aggtatcgac caccgagtcc gggttcagcg agatcgagac gatgccctcc
     4621 tgcgccagcc attcggcgaa gtcggggtgg tcgctggggc cctgcccgca gatgcccacg
     4681 tacttgcccg tggcacggca ggccgtgatc gcgcgcgaga tcatcgcctt gaccgccggg
     4741 tcgcgctcgt cgaagtcctc ggcgagcagt tccaggccgg agtcgcggtc caggcccagc
     4801 gtcagctgcg tcaggtcgtt ggagccgatg gacatgccgt cgaagtgctc caggaactgg
     4861 tcggccagga tggcgttgct cggcacctcg cacatcatga tcagccgcag gccgtcattt
     4921 ccgccattcg cgccgcgctt caggccgcgc tcggcgagca tctcgaccac gcgctgcgcc
     4981 tggcgcaccg tgcgcacgaa gggcaccatg atctcgacgt tgctcaggcc catgtcgatg
     5041 cgcacgcgct tcagggcctc gcactccatc gcgaaggcgt cctggaactc gccgctgatg
     5101 tagcgcgacg cgccgcggaa gcccagcatc gggttctcct cgtccggctc gtagcgcgag
     5161 ccgccgatga gcttcttgta ctcgttggac ttgaagtccg acaggcgcac gatcaccggc
     5221 ttgggccaga acgcggcggc gatcgtcgcg atgccctcgg cgagcttgtc gacgtagaac
     5281 gcgcgcggcg acgcatgccc gcgggcgacg gactcgaccg ccttcttcag gtcggcgtcg
     5341 atgttcggat agtcgaggat cgccttcggg tggacgccga tgttgttgtt gatgatgaac
     5401 tcgaggcgcg cgagcccgac gcccgaattg ggcagctgcg agaagttgaa cgcgagctgc
     5461 gggttgccca cgttcatcat caccttgatc ggcgtgtagg gcatctcgcc gcgggtgacc
     5521 tcggtcacct cggtctcgag caggccgtcg tagacgaagc cggtgtcgcc ctccgagcag
     5581 acgaccgtca cgagcgcgcc gtcggccagc acgtcggtgg cgtcgccgca gcccacgacc
     5641 gccgggatgc ccagctcgcg cgcgatgatg gccgcgtggc aggtgcggcc gccgcggttg
     5701 gtgacgatcg cgctggcgcg cttcatcacc ggctcccagt tcgggtccgt catgtcggcg
     5761 accagcacgt cgccgttctg cacctggtcc atctcgctga tcgagcggca cacgcgcacg
     5821 gtgccggtgc cgatcttctg gccgatcgca cgcccttcgg tcagcaccgt gcccgagccc
     5881 ttgagcttgt agcggtgctc ggcctggccg gcggcgttgc tcttgacggt ctcgggacgc
     5941 gcctggagga tgtacagcag gccgtcgccg ccgtccttgc cccactcgat gtccatcggg
     6001 cggccgtagt gcttctcgat gatcgtggcg tacttcgcca gctcgacgac gtcggcgtcg
     6061 gtcagcgagt agcgattgcg cagctcgggc ggcgtctccg cggtgcgcac gagcctgccg
     6121 tcgcgcgcct tctcctcggc cgaggcgaac tccatgcgga tcagcttgga gccgagcgaa
     6181 cgccggatga tcgggaactt gcccgcggcc agcgccggct tgtggacgta gaactcgtcc
     6241 gggttcacgg cgccctgcac caccgtctcg cccaggccgt aactggacgt gatgaagacg
     6301 acgtcggtga agccgctctc cgtgtcgagc gtgaacatca cgcccgcggc gccgaggtcg
     6361 gagcgcacca tgcgctgcac gccggccgac agcgcgacgt cggagtcggc gtagcccttg
     6421 tggacgcggt agctgatcgc gcggtcgttg tagagggagg cgaagacctc cttcatcttg
     6481 tgcaggacgc cgtcgatgcc gacgacgttg aggaaggtct cctgctggcc ggcgaaggac
     6541 gcgtcgggca tgtcctccgc ggtcgcggaa gagcgcaccg cgaacgaggc gttcgggtcg
     6601 ctcgcggaca ggcgcgcgaa ctcctcgcgc acggccttct ccagttcggg cgggaacggc
     6661 gcgtcctcga tcagcttgcg gatctcggcg ccagcttccg cgagggctcg cacgtcgtcg
     6721 gtgtcgagcg acttcaggcg ccgggcgatg cgctcggtca ggccgccgtg gcggaggaac
     6781 tcgcggaacg cgtgcgccgt cgtggcgaag ccgccgggca cccgcacgcc ggaggcggcg
     6841 agctggctga tcatctcgcc gaggctggcg ttcttgccgc cgaccgcctc gacgtcggtc
     6901 atcctcagtt gttcgaacgg tacgaccagg gcggtcgcca ggttgggttg ggacacgggg
     6961 aaactccttg atgatggaaa accgggttcc gcccggcgcc cccgaggccg cgcgcttcga
     7021 ctctttggag ttcgagttgt cgatcgaagc gggccatggg acgcgtgcgg atgggattta
     7081 ggcgacagtt gggcgggatt ctacggagga tctgcctatc atccgtagga caacccgcca
     7141 aatcactccc cgaacatgcc caatcgcacg gtcttcttca tctccgacgg caccgggatc
     7201 accgccgaga ccttcggcaa ctcgatcctc gcccagttcc cgggccgccc gcgcaaggtg
     7261 cgccggccct tcatcgacac cgtggacaag gccgccaagg tggtcgagga gatcaaccac
     7321 accttcgtcg ccgagggcaa gaagccgatc gtcttcatca cgatggccag cgaggacgtg
     7381 cggcaggcgc tcaccggccc cgagtgctcc ggcgtgatca tggacatgtt ccgcaccttc
     7441 gtggagccgc tggaggtcga gttcggcatg aagtcgaacc accgcgtcgg ccgcttctcg
     7501 gacgccgcca agagcgagga gtacaacgag cgcatcgagg cgatcaactt ctcgctcgcg
     7561 cacgacgacg gccagtcctc gcgcaacctg gcgcaggccg acgtgatcct ggtgggcgtc
     7621 agccgctgcg gcaagacgcc cacgacgctg tacctggcca tgcagcacgg catcaaggcg
     7681 gccaactatc cgctgatccc ggaggacttc gagcgcggcc agttcccgtc ctcgctcgcc
     7741 ccgttcaagg ccaagtgctt cggcctgacg atcgatcccg agcgcctcac gcagatccgc
     7801 aacgagcgcc gcccttcgag caagtacgcc gcgctcgaga actgccgcta cgaggtgcgc
     7861 gaggccgagt ccatgatgaa gcgcgagggc atctcctggc tgtcgtcgac ccacaagtcg
     7921 atcgaggaga tcgccgcgac catcctgcgc gacatccgcc cggacaagct gatctactga
     7981 ccgcgggcga cgagccggcg cgccgactcg tgcaggcaga tcgcggccgc cgcggcgacg
     8041 ttgagcgact cctcgccgcc gggctgggcg atgcgcacgc gcaggtcgca ggcggcggcg
     8101 acggcgtccg acatcccctg accttcgtgc ccgaacaccc acgcgagcgg atcgggcagc
     8161 gccgcctcgt gcagccaccc ggcgtcgcgc aggtgcgtgc cgaccatggg tacgccgagc
     8221 gctcgcagct cgtccaggcc ggcgccctcg acgagcgaca gcccgaaatg cgcgcccatg
     8281 ccggcgcgca gcaccttggg cgaccacagc gccaccgtgc ccttgagcgc gaccacctgc
     8341 gcgaagccca tcgccgacgc cgtgcgcagg aggctgccgg cgttgccggg gtcctgcagc
     8401 cggtcgagca ccacggtccg cacgccgggg cgcagggtcg cgtcgcggcc tgcgtcgatc
     8461 aggaagccga gcggcgccgg cgactccagg ccgctcagct cgcgcatcag cgcgtcgggg
     8521 acgcagacga cctcgggcgc cgtgcgggcc agcgcggcga gctcgggcct cgcccagccc
     8581 gaggccgtca cgatcgcgcg cagcg
//
LOCUS       DASVLN010000078         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_273099, whole genome shotgun sequence.
ACCESSION   DASVLN010000078 DASVLN010000000
VERSION     DASVLN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273099"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VF453_01740"
     CDS             complement(<1..468)
                     /locus_tag="VF453_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686392.1"
                     /translation="MANASAPAPGPASDAHASFVGKLVADPKQPPNTLLLTGFLGASS
                     DEGHTRLYFDPQLSDYVEIPDDAILHRQDVPAGLSPLGASYVWVRRDATLIHGPIGPD
                     RLKASFLEGRLARDHAAAATGGFVPHIPPQTAPVLCDPTRVGPGCHPTNALACA"
     gene            complement(765..1586)
                     /locus_tag="VF453_01745"
     CDS             complement(765..1586)
                     /locus_tag="VF453_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686393.1"
                     /translation="MKNRLAALVAAGLALVGGPALAAASASATLSGLTVTLIDLYPSD
                     GGKAGVQFSTLYGGAEVGTYSYDSSLPDPSSDAAFSSYPLSGAANILSGAGRASAGAS
                     MGGDPFAGTGLAALLAAAPSAAVNGYSTAIAQYGLGSGGSTTLFSLAPGTLMDISGTV
                     TLSASSDTAGMEYADAGLQLKLFGFGNVSQNASATFAVDASSGSGMQTLTEHFDIAFT
                     GQRDAITEGYFYGSLLAEASTSDPPPAVPEPAGALPWLAGVATLAAWGRRRVRRG"
     gene            complement(1832..>2132)
                     /locus_tag="VF453_01750"
     CDS             complement(1832..>2132)
                     /locus_tag="VF453_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7686394.1"
                     /translation="SADWNNLMGYVSRKGPTPDLAASERYYDAALRIDPQHRNTLEYS
                     GELYLMKGDLPKAQARLAALAGACASNCPQYAQLKGAIARYQANGNRYIADGNGW"
BASE COUNT          285 a          721 c          834 g          292 t
ORIGIN      
        1 ggcacaggcc agcgcgttgg tcgggtggca gcccgggccg acccgcgtcg ggtcgcagag
       61 cacgggcgcc gtctgcggcg gaatatgcgg cacgaacccg ccggtcgcgg ccgccgcgtg
      121 gtcgcgcgcg aggcgcccct ccaggaacga ggccttcagc cggtcgggcc cgatcgggcc
      181 gtggatcagc gtggcgtcgc gccgcaccca gacgtagctc gcgcccagcg gcgacagccc
      241 cgccggcacg tcctgccggt ggaggatggc gtcgtcgggg atctcgacgt agtcggacag
      301 ctgcggatcg aagtacagcc gggtgtggcc ctcgtcggac gaggcgccga ggaagcccgt
      361 gagcagcagc gtgttgggcg gctgcttggg gtcggcgacg agcttgccga cgaacgacgc
      421 gtgcgcgtcg gacgcggggc cgggagcggg agcggaagcg ttggccatcg tgtctccttt
      481 cgacgtggcg gacgggcgcg agcgtgcggc accggcgccg aggcgtcggg ctcgaacgat
      541 cgaagacgcc gcgcggccgt gcggcgcggg cgtgtcaccg cggctcgcgg cgatgggcgg
      601 actgtctcgc gccggcgcac gccgccccat ccccaactcc gtgggcggga gcgttcaagt
      661 tagggatatg cgggcacgat cccgtacccc ggtcgtatcc gggcgagata cctgtcaggg
      721 tacgggtatg ccggcagcga tgccggcggg gcgcatcggg tgcgtcagcc gcggcggacg
      781 cgtcgtcgcc cccaggccgc gagcgtcgcg acgcccgcca gccagggtag cgcgccggcc
      841 ggctcgggca cggcgggcgg cggatcgctg gtgctcgcct cggccagcag gctgccgtag
      901 aagtagccct cggtgatcgc gtcgcgctgg ccggtgaagg cgatgtcgaa gtgctcggtc
      961 agggtctgca tgccggagcc gctcgaggcg tcgacggcga aggtcgcgct cgcgttctgc
     1021 gagacgttgc cgaagccgaa gagcttgagc tgcaggccgg cgtccgcgta ttccatgccg
     1081 gcggtgtccg acgacgcgct cagggtgacg gtgcccgaga tgtccatcaa cgtgcccggc
     1141 gcgagcgaga acagcgtggt cgagccgccg ctgccgagcc cgtactgggc gatcgccgtc
     1201 gagtagccgt tcaccgccgc ggacggcgcg gccgcgagca gtgcggccag gccggtgccg
     1261 gcgaaggggt cgccgcccat cgaggcgcct gccgacgccc ggcccgcgcc cgacaggatg
     1321 ttcgccgcgc cggacagcgg gtagctcgag aaggcggcgt ccgacgacgg gtcgggcagg
     1381 ctcgagtcgt agctgtacgt gcccacctcg gcgccgccgt agagcgtcga gaactggacg
     1441 ccggccttgc cgccgtcgga cgggtagagg tcgatcaggg tgacggtcag gccgctcagc
     1501 gtggcgctgg ccgaggccgc ggcgagggcc gggccgccga cgagcgcgag gccggccgcg
     1561 accagcgcgg caagacgatt cttcatgggt tcttccccgg gacgtgacgg tgggggacgc
     1621 cggcgggcgc cggcgtcgac gcgaggaact gtagtccccg tccaccgccg agctgggtcg
     1681 catcccctag aacaaggcgc cgcccgaccg ccgcattgac atgccgttac acgtatggcg
     1741 gtcatgtcac gaaacgcatg gctttcctgc cacacacgtc ccgggccccg ccggacgggc
     1801 gcgggacgga ctcacggctt cgtgacgcga ctcaccagcc gttgccgtcg gcgatgtacc
     1861 tgttgccgtt ggcctggtag cgcgcgatcg cgcccttgag ctgcgcgtac tgcgggcagt
     1921 tggacgcgca tgcgcccgcg agcgccgcga gccgcgcctg cgccttgggc aggtcgccct
     1981 tcatcaggta gagctcgccc gaatattcca gcgtgttgcg gtgctgcgga tcgatgcgca
     2041 gcgccgcgtc gtagtagcgc tccgaggccg cgaggtcggg cgtcgggccc ttgcgcgaga
     2101 cgtagcccat gaggttgttc cagtcggcgc tg
//
LOCUS       DASVLN010000079         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_218331, whole genome shotgun sequence.
ACCESSION   DASVLN010000079 DASVLN010000000
VERSION     DASVLN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218331"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VF453_01755"
     CDS             <1..829
                     /locus_tag="VF453_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX7686395.1"
                     /translation="RLRGRGPAFRLEVRRGAGSYVCGEEMALLESLEGRRGVVRAKPP
                     LPAIHGLFGWPTVINNVITLASVPRILAGGAVAYHAFGAGRSRGTLPFQLAGNVRHGG
                     LFEAAFGLTLRELMVDIGGGTASGRPIKAVQVGGPLGAYVPESAWDVPIDYEAWAALG
                     LGVGHGGIVIHDDTADLAGLARYAMEFCALESCGKCTPCRIGAVRGVEVIERIVAAPA
                     SAKPAQVALLRDLCDTMVSGSLCAMGGMTPFPVLSALDHYPEDFGLPRRTPAAAGEP"
     gene            829..3762
                     /gene="fdhF"
                     /locus_tag="VF453_01760"
     CDS             829..3762
                     /gene="fdhF"
                     /locus_tag="VF453_01760"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX7686396.1"
                     /translation="MLNLNDPRPDIDLGTPASASAELVSLTIDGVAVQARAGTSVMRA
                     AMEAGIAVPKLCATDSLEPFGSCRLCLVEIAGRRGTPASCTTPVEAGMAVVTQSERLA
                     KLRRGVMELYISDHPLDCLTCSANGDCELQDMAGAVGLREVRYGMQGANHLADRKDTS
                     NPYFEYDPAKCIVCNRCVRACEETQGTFALTISGRGFGSRVSAGMDEPFLESECVSCG
                     ACVQACPTATLQEKSVVALGMPTKSVTTTCAYCGVGCGFKAEVKGTQVVRMTPWKDGK
                     ANEGHSCVKGRFAWGYATHADRITTPMIRASIDEPWQEVSWDVAIAHAASEFKRLQAK
                     YGRDSIGGITSSRCTNEETYLVQKLVRSAFGNNNVDTCARVCHSPTGYGLGVTYGTSA
                     GTQTFKSVAKSDVIVVIGANPASAHPVFASRMKRRLRQGAKLIVIDPRRTELVDGPHV
                     HADFHLQLRPGTNVAVLNALAHVIVTEGLLDEAFIAGRCEDRAFAQWREFVARPENSP
                     EATEQETQVPAAAMRGAARLYATGRADGTPATLAADARRPNGAIYYGLGVTEHAQGST
                     AVIGIANLAMATGNIGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHIGGLEVRTQ
                     FEDAWGVTLQSEPGLRIPNMFDAALAGSFKGLYCQGEDIVQSDPNTQHVGAALRAMEC
                     VVVQDIFLNETAKYAHVFLPGSSFLEKDGTFTNAERRISLVRKVMEPLSGVGDWEATM
                     RLSNALGYPMTYSHPREIMDEIARLTPTFAGVSFDKIERLGSVQWPCNEQTAEAGTDI
                     MHADGFVRGKGRFVITQYVASDEKPTPRFPLLLTTGRILSQYNVGAQTRRTPNSVWHD
                     EDKLEIHPQDALDRGVKDGDWVGIASRAGETVMRATVTERVPPGVVYTTFHFPESGAN
                     VITTDSSDWATNCPEYKVTAVQVMPVAQPSSWQRAYRDFDAEQRRFLADAKLGRGVTA
                     PAGGK"
     gene            3759..>4200
                     /locus_tag="VF453_01765"
     CDS             3759..>4200
                     /locus_tag="VF453_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX7686397.1"
                     /translation="MTRTGTLPDDEAPRPADDGGAARVDVVVRRDGAVRAAEDWLADE
                     VPVALVFNGLSHAVMLATPLALEDFALGFGLTEGLLARPDELYDVEVVPDPAGLRVEM
                     TVASACFARMKDRRRSMAGRTGCGLCGTESLEHVEPPLPPLAPCD"
BASE COUNT          605 a         1527 c         1505 g          563 t
ORIGIN      
        1 gcgcctgcgc ggccgcggcc cggccttccg cctcgaggtg cggcgcggcg ccggctcgta
       61 cgtgtgcggc gaggagatgg cgctgctcga gagcctggag gggcgccgcg gcgtcgtgcg
      121 cgccaagccg ccgctgccgg cgatccacgg cctgttcggc tggccgaccg tcatcaacaa
      181 cgtcatcacg ctcgcgagcg tgccgcgcat cctcgcgggc ggcgcggtcg cgtaccacgc
      241 cttcggcgcc gggcgctcgc gcggcacgct gccgttccag ctcgccggca acgtcaggca
      301 cggcggactg ttcgaggccg cgttcggcct gacgctgcgg gagctcatgg tcgacatcgg
      361 cggcggcacc gccagcggcc ggccgatcaa ggcggtgcag gtcggcggcc cgctcggagc
      421 ctacgtgccc gagtcggcct gggacgtccc catcgattac gaggcctggg ccgcgctcgg
      481 cctgggcgtc gggcacggcg gcatcgtgat ccacgacgac accgccgacc tcgccggcct
      541 cgcgcgctac gcgatggagt tctgcgcgct cgagtcctgc ggcaagtgca cgccctgccg
      601 catcggggcg gtgcggggcg tggaggtcat cgagcgcatc gtcgccgccc ccgcgtcggc
      661 caagccggcg caggtggcgc tgctgcgcga cctgtgcgac accatggtct cgggcagcct
      721 gtgcgcgatg ggcggcatga cgccgtttcc ggtgctgtcc gcgctcgacc actatcccga
      781 ggacttcgga ttgccgcggc gcacgccggc cgcggcggga gaaccgtgat gctgaacctc
      841 aacgatccgc ggccggacat cgacctcggc acgcccgcga gcgcgtccgc cgagctcgtc
      901 tccctgacca tcgacggcgt cgccgtgcag gcgcgcgccg gcacctcggt gatgcgcgcg
      961 gcgatggaag ccggcatcgc cgtgcccaag ctgtgcgcga ccgacagcct cgagccgttc
     1021 ggctcgtgcc ggctgtgcct ggtggagatc gccggccgcc gcggcacgcc cgcgtcgtgc
     1081 acgacgccgg tcgaggcggg catggcggtc gtcacgcaga gcgagcgcct ggccaagctg
     1141 cgccgcggcg tgatggagct ctacatctcc gaccatccgc tcgactgcct gacctgctcg
     1201 gccaacggtg actgcgagct gcaggacatg gccggcgccg tcggcctgcg cgaggtgcgc
     1261 tacggcatgc agggcgccaa ccacctggcc gaccgcaagg acacgtctaa cccgtacttc
     1321 gagtacgacc ccgccaagtg catcgtctgc aaccgctgcg tgcgcgcctg cgaggagacg
     1381 cagggcacct tcgcgctgac catctcgggc cgcggcttcg gctcgcgggt gtcggccggc
     1441 atggacgagc ccttcctcga gagcgagtgc gtcagctgcg gcgcctgcgt gcaggcctgc
     1501 ccgacggcga cgctgcagga gaagtcggtc gtcgcgctgg gcatgccgac gaagagcgtg
     1561 acgacgacct gcgcctattg cggcgtgggc tgcggcttca aggccgaggt caagggcacg
     1621 caggtcgtgc gcatgacgcc ctggaaggac ggcaaggcca acgagggcca ctcctgcgtc
     1681 aagggccgct tcgcctgggg ctacgcgacg cacgccgacc gcatcacgac gccgatgatc
     1741 cgcgcctcga tcgacgagcc gtggcaggag gtctcgtggg acgtcgcgat cgcgcacgcg
     1801 gcctcggagt tcaagcgcct gcaggccaag tacggccgcg actcgatcgg cggcatcacg
     1861 tcctcgcgct gcacgaacga ggaaacctac ctcgtgcaga agctcgtgcg ctcggccttc
     1921 ggcaacaaca acgtcgacac ctgcgcccgc gtgtgccact cgccgaccgg ctacggcctg
     1981 ggcgtcacct acggcacctc ggccggcacg cagacgttca agtcggtcgc caagtccgac
     2041 gtgatcgtcg tcatcggcgc gaacccggcc tcggcgcacc cggtgttcgc ctcgcgcatg
     2101 aagcgccgcc tgcgccaggg cgcgaagctg atcgtgatcg atccgcggcg caccgagctg
     2161 gtcgacggcc cgcacgtcca cgccgacttc cacctgcagc tgcggcccgg caccaatgtc
     2221 gcggtgctca acgcgctcgc ccacgtcatc gtgaccgagg gcctgctcga cgaggcgttc
     2281 atcgccggcc gctgcgagga ccgcgccttc gcgcaatggc gcgagttcgt cgcacggccg
     2341 gagaactcgc ccgaggcgac cgagcaggag acgcaggtgc cggccgcggc gatgcgcggc
     2401 gccgcgcggc tgtatgcgac cggccgcgcc gacggcaccc ccgccacgct ggccgccgac
     2461 gcgcgacgcc cgaacggcgc gatctactac ggcctgggcg tgaccgagca cgcgcaggga
     2521 tcgaccgcgg tcatcggcat cgccaacctc gcgatggcca cgggcaacat cggccgcgag
     2581 ggcgtcggcg tgaacccgct gcgcggccag aacaacgtcc agggctcgtg cgacatggga
     2641 tcgttcccgc acgagctgcc cggctaccgc cacatcggcg gcctggaggt gcgcacgcag
     2701 ttcgaggacg cgtggggcgt gacgctgcag tcggagccgg ggctgcgcat ccccaacatg
     2761 ttcgacgccg cgctcgcggg ctcgttcaag ggcctgtact gccagggcga ggacatcgtg
     2821 cagtccgacc ccaacaccca gcacgtcggc gcggcgctgc gcgcgatgga gtgcgtggtc
     2881 gtgcaggaca tcttcctcaa cgagaccgcg aagtacgccc acgtcttcct gccgggatcg
     2941 tcgttcctcg agaaggacgg caccttcacg aacgccgagc gccgcatctc gctcgtgcgc
     3001 aaggtgatgg agccgctgtc gggcgtgggc gactgggagg cgacgatgcg cctgtccaac
     3061 gcgctcggat accccatgac ctactcgcac ccgcgcgaga tcatggacga gatcgcgcgc
     3121 ctgacgccga ccttcgcggg cgtgagcttc gacaagatcg agcgccttgg cagcgtgcag
     3181 tggccgtgca acgagcagac cgccgaggcc ggcaccgaca tcatgcacgc ggacggcttc
     3241 gtgcgcggca agggccgctt cgtgatcacg cagtacgtcg cctcggacga gaagccgacg
     3301 ccgcgcttcc cgctgctgct gacgaccggg cgcatcctgt cgcagtacaa cgtcggcgcg
     3361 cagacgcgcc gcacgcccaa ctccgtgtgg cacgacgagg acaagctcga gatccatccg
     3421 caggacgccc tggaccgcgg cgtgaaggac ggcgactggg tcggcatcgc gagccgcgcg
     3481 ggcgagacgg tgatgcgcgc gacggtcacc gagcgggtgc cgccgggggt ggtctacacg
     3541 actttccact tccccgagtc gggcgccaac gtcatcacga ccgacagctc cgactgggcg
     3601 accaactgtc ccgagtacaa ggtgaccgcc gtgcaggtga tgccggtggc gcagccgtcg
     3661 tcgtggcagc gcgcctaccg cgacttcgac gccgagcagc gccgcttcct cgccgatgcg
     3721 aagctcgggc gcggcgtgac cgcgccggcc ggaggcaaat gacgcgcacc ggcacgctcc
     3781 cggacgacga agcgccccgg ccggcggacg acggcggcgc cgcgcgcgtg gacgtcgtcg
     3841 tgcgacgcga cggcgccgtg cgcgccgccg aggactggct cgccgacgag gtgccggtgg
     3901 cgctcgtgtt caacggcctg tcgcacgcgg tgatgctggc cacgccgctc gcgctcgagg
     3961 acttcgcgct cggcttcggc ctcaccgagg ggctgctcgc gcggcccgac gagctctacg
     4021 acgtggaggt cgtgcccgac ccggcggggc tgcgcgtcga gatgaccgtc gcgagcgcct
     4081 gcttcgcgcg catgaaggat cgtcgtcggt cgatggccgg ccgcaccggc tgcgggctgt
     4141 gcggcaccga gagcctggag cacgtggagc cgccgctgcc gccgctcgcg ccctgcgacg
//
LOCUS       DASVLN010000080         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_433961, whole genome shotgun sequence.
ACCESSION   DASVLN010000080 DASVLN010000000
VERSION     DASVLN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433961"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /gene="nth"
                     /locus_tag="VF453_01770"
     CDS             complement(<1..695)
                     /gene="nth"
                     /locus_tag="VF453_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX7686398.1"
                     /translation="MTPADIDAFFATLQRANPEPASELHYASVFELLAAVLLSAQATD
                     VSVNKATRTLFAKAPTPQRMLALGAEGIEAHVRTIGLYRTKTKHLLETCRILVERYGG
                     EVPRDRAALEALPGVGRKTANVVLNVAFGEPTLAVDTHVFRVANRTGLAPGRTPLAVE
                     LGLLERIPAKYLDDAHHWLILHGRYVCLARMPQCGRCAVSAQCDWFRALPADARPKPG
                     QDAPVARALRAAG"
     gene            complement(703..>1541)
                     /locus_tag="VF453_01775"
     CDS             complement(703..>1541)
                     /locus_tag="VF453_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7686399.1"
                     /translation="LAARDLPPLTFTGLRFLLGAAIVAPLAWREWRSLGASGRRPRRR
                     DLGYIGLLGGVVFLGAALQQVALLRASVTHTGFLTALYIPQVPLLAWLALGQRPHWTI
                     APAVAGCLAGTWMLTAAAGTALALQPGDAWAIASSVPWAVHVLLVGHAANRLSGPMVV
                     ACGQFAACGALALAAGLAFEAPTAAGVARTLGPIAYTGAVSVALGYTLQVVAQREARP
                     ADSAIVLSSETVFAAAFGATFLGERLDARGMAGCALILGCVLMVQLQPLVRSWTRRRS
                     TA"
BASE COUNT          221 a          559 c          593 g          168 t
ORIGIN      
        1 atccccgcgg cgcgcagggc ccgggcgacg ggggcgtcct ggcccggctt cgggcgcgcg
       61 tcggcgggca gcgcgcggaa ccagtcgcac tgcgccgaga ccgcgcagcg cccgcactgc
      121 ggcatgcgcg cgaggcagac gtagcggccg tgcaggatca gccagtggtg cgcatcgtcg
      181 aggtacttcg ccggaatgcg ctcgagcagc ccgagctcga ccgccaacgg cgtcctgccc
      241 ggcgccagcc cggtgcggtt ggcgacgcgg aagacgtgcg tgtcgaccgc cagcgtcggc
      301 tccccgaacg cgacgttgag cacgacgttg gccgtcttgc ggccgacgcc cggcagcgcc
      361 tccagcgcgg cgcggtcccg cggtacctca cccccgtatc gctcgacgag gatgcggcag
      421 gtctccagca ggtgcttcgt cttcgtccgg tagaggccga tcgtgcgcac gtgggcctcg
      481 atgccctcgg cgccgagcgc cagcatgcgc tgcggcgtcg gcgccttcgc gaacagcgtg
      541 cgcgtggcct tgttgacgct gacgtcggtg gcctgcgcgg acagcagcac ggcggcgagc
      601 agctcgaaga cgctcgcgta gtgcagctcg gaggcgggct cgggattcgc gcgctgcagc
      661 gtcgcgaaga aggcgtcgat gtcggcaggg gtcattcgcg tctcaggcgg tggatcggcg
      721 tcgcgtccag gagcggacga gcggctgcaa ttggaccatc agcacgcagc cgaggatcag
      781 cgcgcatccg gccatcccgc gggcgtcgag ccgctcgccc aggaacgtcg caccgaaggc
      841 ggcggcgaac acggtctcgg acgacagcac gatcgccgag tcggccgggc gcgcctcgcg
      901 ctgggcgacc acttgcagcg tgtagccgag cgcgaccgag acggcaccgg tgtaggcgat
      961 gggcccgagc gtgcgcgcga cgccggccgc ggtcggcgcc tcgaacgcga ggccggccgc
     1021 caacgccagc gcgccgcagg ccgcgaactg cccgcaggcg acgaccatcg gcccgctcag
     1081 ccggttggcc gcgtgaccca cgagcagcac gtgcaccgcc cagggcacgc tgctcgcgat
     1141 cgcccaggcg tcgccgggct gcagcgcgag cgcggtgcca gcagcggcgg tcagcatcca
     1201 tgtgcccgcg aggcaaccgg cgacggcggg cgcgatcgtc cagtgcggac gctgccccag
     1261 cgccagccag gcgagcagcg gcacctgcgg gatgtagagc gcggtcagga agccggtgtg
     1321 cgtgacgctc gcgcgcagca gcgcgacctg ctgcagcgcc gcgccgagga acaccacccc
     1381 gcccagcagg ccgatgtagc cgaggtcgcg ccgccgcggc cgccggccgc tcgcaccgag
     1441 cgagcgccat tcgcgccagg ccaggggcgc gacgatcgcg gcgccgagga ggaagcgcag
     1501 gcccgtgaag gtcagcggcg gcaggtcgcg cgcggccagg g
//
LOCUS       DASVLN010000081         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_379915, whole genome shotgun sequence.
ACCESSION   DASVLN010000081 DASVLN010000000
VERSION     DASVLN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379915"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..612)
                     /locus_tag="VF453_01780"
     CDS             complement(175..612)
                     /locus_tag="VF453_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7686400.1"
                     /translation="MNFRKPRPEDPEINLIPFIDVLLVILIFLMLSTTYSKFTELQVT
                     LPAADAAAARDREAEIIVSVAADGRYAVDHQAVDGRNVDQLTAALQAAASQRKEPFLI
                     ISADAMSTHQAVINVLEAARRAGLSRMTFAAQATNGAGGTASP"
     gene            complement(626..1261)
                     /locus_tag="VF453_01785"
     CDS             complement(626..1261)
                     /locus_tag="VF453_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7686401.1"
                     /translation="MFKILLEAGWPIVPLFLCSVIAVAVVVERFMALRAARVAPGYLL
                     GEVLAVTRNGLPAPDVIAKLADNSLLGNVLAAGLKQLALDPRSSEQQLRQTFEGAGRD
                     AAHKLDRYLNTLGTVAAVAPLLGLFGTVVGMIEIFGSQTGSAGSANPVELAHGISTAL
                     YNTAGGLLVAIPSLMFYRYFRGRVDDYLHTLEQSSEQLLAQLVRLGRPGLK"
BASE COUNT          252 a          565 c          655 g          266 t
ORIGIN      
        1 gccagcgcgc gcgcgagcgg cgtcgtgcgc ggctgccacc acgagcgctg gagccggtgg
       61 gcgaacgtct cggtcatcgc ggcgggggag gtcgtcggtc ggggcggggc caatgaatcg
      121 gggccggcga tcgagcggcg atcggcggcc ccggcggttc ggatcggcgg gcgatcaggg
      181 cgaggccgtg ccgcccgcgc cgttggtcgc ctgcgcggcg aaggtcatgc gcgacaggcc
      241 cgcgcggcgc gccgcctcga gcacgttgat caccgcctgg tgcgtcgaca tcgcatccgc
      301 cgagatgatc aggaagggct ccttgcgctg gctcgcggcc gcctgcagcg cggcggtcag
      361 ctggtcgacg ttgcggccgt ccaccgcctg gtggtcgacg gcgtagcggc cgtcggcggc
      421 cacggagacg atgatctcgg cctcgcggtc gcgcgcggcc gcggcatcgg cggccggcag
      481 cgtgacctgg agctcggtga acttcgaata ggtcgtcgac agcatcagga agatcaggat
      541 caccagcagc acgtcgatga acgggatgag gttgatctcg ggatcttccg ggcgaggctt
      601 gcggaagttc atcgcggcgc ccgcctcact tcagaccggg gcgcccgagg cgcaccagct
      661 gggcgagcag ctgctcggac gactgctcca gcgtgtgcag gtagtcgtcg acgcggccgc
      721 ggaagtagcg gtagaacatc agcgacggga tcgccaccag caggccgccg gcggtgttgt
      781 acagcgcggt cgagatgccg tgcgcgagtt ccaccgggtt ggcgctgccg gccgagccgg
      841 tctgcgagcc gaagatctcg atcatgccca ccacggtgcc gaacaggccc agcagcggcg
      901 ccacggcggc caccgtgccc agcgtgttga ggtagcggtc gagcttgtgg gcggcgtcgc
      961 ggccggcgcc ctcgaaggtc tggcgcagct gctgctccga agagcgcggg tcgagcgcga
     1021 gctgcttcag gccggcggcc agcacgttgc ccagcagcga gttgtcggcc agcttcgcga
     1081 tgacgtcggg cgccggcagt ccgttgcgcg tgaccgccag gacctcgccg aggaggtagc
     1141 cgggcgcgac gcgggccgcg cgcaaggcca tgaaccgttc gacgacgacc gccaccgcga
     1201 tcacggagca gaggaacagc ggcacgatgg gccagccggc ctccagtagg atcttgaaca
     1261 aaatcgacct ttcgacaatg cggatgcgcc ggccgcgtcg tcgcggcggg ccggtcgcgg
     1321 cgctcggcgg gtgccgtcgc cagcgcggat tatggcgtga cggcgcgcgc tttccagcgc
     1381 ggtcgcggtg tgcgtttgta gccggccagc gatccacacg atctgtggaa aactttgtgg
     1441 gtaagtgggg ccgacggccc ccggagccgc gattttccgt gcttcgaact ggattgcctt
     1501 aaaatcgggc actgaaaaat gcttgtgaat caatggcttg cgtgttaata gcgtgcgcgt
     1561 ccgcggtatc ggcggccggc ctcggagtct tctgaaaatg tggagtacta gggtttgtcc
     1621 cctcgattcg tgaccgggag ccgtgccaat gcctgatcca ttcgccgtcc cgggcttcgg
     1681 gcccgcgccg tccccgcggc cgtcgtcgtc cgccgcgccg ccgcggcccc aggcgtgg
//
LOCUS       DASVLN010000082         5255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_273367, whole genome shotgun sequence.
ACCESSION   DASVLN010000082 DASVLN010000000
VERSION     DASVLN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273367"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1357)
                     /gene="gspF"
                     /locus_tag="VF453_01790"
     CDS             complement(143..1357)
                     /gene="gspF"
                     /locus_tag="VF453_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEX7686402.1"
                     /translation="MPAYKYEALDIAGKTANGIVEAESMKAARAHLRGLSLVPLGLAP
                     VAVADDSRPAGRFSRKAFSSSGLAVWTRQLAGLVAAGLPLERALTALSDEAEVQSQRD
                     LVAHLRSEVNGGATFARALGTVPREFDDVYRAVVAAGEQSGALGRVLERLADDLEERQ
                     ALKGKLLGAALYPIIVSIIAVVIVTFLVTYVVPQVASVFASGKHALPLLTQLMLGISA
                     FVRQWGLVALGVLVAGGFLFSTALRQPAFRTRFDAMTLNLPLAGRLARGYNAARFAGT
                     LAMLAGSGVPILKALQAAAETLSNHAMRADAMEALVQVREGAPLASALAAHKRFPGLL
                     AMFARLGEQTGQLPVMLDRAARQLGNEVQRRAFALATLLEPLLIVAMGGVVMLIVLSV
                     LLPIIQLNSFVK"
     gene            complement(1397..2818)
                     /gene="gspE"
                     /locus_tag="VF453_01795"
     CDS             complement(1397..2818)
                     /gene="gspE"
                     /locus_tag="VF453_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEX7686403.1"
                     /translation="MGARHPLPYAWAKTHLMLLEDDGRERTLHVSEGTSRTALAEVLR
                     LHAIDRIERSAPATLSARIAEAYSGGEGSAAAVVGEVESAVDLSRMMQELPAVEDLLE
                     AANDAPIIRMLNALLTQAAKDGASDIHIEPYERSSSVRFRVDGTLREVVQPNKALHAA
                     LISRLKIMAELDISEKRLPQDGRISLRIGGRAIDVRVSTLPSAHGERAVLRLLDKGEA
                     KFSLEALGMSGDVLHEFNRLVNQPHGIVLVTGPTGSGKTTTLYASLSRLDTRTSNVLT
                     VEDPVEYELPGIGQTQVNPKIDLTFAKALRAILRQDPDVVMIGEIRDFETAQIAIQAS
                     LTGHLVLATVHTNDAPSTVARLIDMGVEPFLLSSSLLGVLAQRLVRKLCEFCKREDDE
                     HRWHPVGCEQCGHSGYKGRTGVYELMVVDEAIQALVHGRAPEQQIIAAARANGMRTMR
                     EDGERLVAEGVTSLEEVVRVTRD"
     gene            complement(2853..>5255)
                     /gene="gspD"
                     /locus_tag="VF453_01800"
     CDS             complement(2853..>5255)
                     /gene="gspD"
                     /locus_tag="VF453_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX7686404.1"
                     /translation="PVQAAPHAAAAQASAGEDAVPTARARNTPVTLNFVNADIEAVSR
                     AIGAMLNRDILVDPRVKGTITVYNDKPQPLSEAYRSYLAALRGLGFTVVEAGGFLKVV
                     PEADAKLQTGVVSVGTPARSGDQVLTQIFKLQHENPNNLVAVLRPLITANNTINASPG
                     SNSLVITDYADNLQRLGRIIEALDQPSGSELEVVPLKNAVASDIVQLVQKLVDGGAVQ
                     TPGVPGQTAQTSILVDPRTNSLIIRAANATRMAQARMIVDKLDQPTAGGGPAGNIWVV
                     YLKNADATKIAEVLRAAIASGSVGGGGGSSGGTGGGSGNASGQTSAFGGSNRDATSSS
                     SMTSTSGTTGGNASPQSTNPVSASAHPSTGGQVQADPATNSLIITASEPLYRQMRTVI
                     DQLDVRRAQVYVEALMVTMDETKAAQMGFQWQNLFGRKGDSTLTGAGTNFGSQSTLNN
                     ILTGSASLIKGTSGVSSTAQTLVGSGTNAISNGLNIGIVKNLGSYYTLGALAQFLETN
                     TGANVLATPNLIAMDNEEAKIVVGQNIPLVTGSYTNTGSSSSSVNPFQTVERQDVGIT
                     LRIKSSIGENGTVRMTIYEENSSVAPGTGGQTTNKTSYEGYVTVDDGSLIVLGGLISD
                     DYNDEVDQVPLLGNIPLIGNLFKSQNRTRSKRNLMLFLRPVVMRSQSDADAITLNRYD
                     AIRRQQETTQPAKNIVLRVNDAPVVPRIGADTRVLPTPQDSQEGSVKMQNVDPLPDLH
                     RPGAIYGPGAAQPLPQQAPQPTFVPLGQMTPVAPQPGSSTTTTTTTTTVAPAPAASVP
                     TK"
BASE COUNT          679 a         1793 c         1955 g          828 t
ORIGIN      
        1 tgcacagcgc ggcgatccac gggcgcgacg gccgggtaac gggcgtgtcg gcgcgtgaac
       61 cggcaaacgg cgctcccgcc gagcgggccg cggccatcag gacaggggat tgcgggacgc
      121 cgcgccggcg cgccgcgcgc cgtcacttca cgaaggagtt cagctggatg atcggcagca
      181 gcaccgacag cacgatcagc atcaccacgc cgcccatcgc gacgatcagc agcggctcca
      241 gcagcgtcgc gagtgcgaag gcgcggcgct gcacctcgtt gccgagctgg cgcgccgcgc
      301 gatcgagcat cacgggcagc tggccggtct gctcgcccag gcgcgcgaac atggcgagca
      361 agccggggaa gcgcttgtgc gccgcgagcg cggaggcgag cggcgcgccc tcgcgcacct
      421 gcaccagtgc ctccatcgcg tccgcccgca tggcgtggtt ggacagcgtc tcggccgctg
      481 cctgcagcgc cttcaggatc ggcacgcccg agcccgcgag catcgccagc gtgccggcga
      541 agcgcgccgc gttgtagccg cgcgccaggc gcccggccag cggcaggttg agcgtcatcg
      601 cgtcgaaccg ggtgcggaac gcgggctggc gcagcgccgt ggagaacagg aagccgcccg
      661 ccaccagcac gcccagcgcg accaggcccc actggcgcac gaacgcgctg atcccgagca
      721 tcaactgcgt gagcaagggc agcgcgtgct tgccgctcgc gaagacggac gcgacctgcg
      781 gcaccacgta ggtgacgagg aaggtgacga tgacgacggc gatgatgctg acgatgatcg
      841 ggtacagcgc ggcgccgagc agcttgccct tgagcgcctg gcgctcctcg aggtcgtcgg
      901 ccaggcgctc gagcacgcgc ccgagcgcgc cgctctgctc gccggcggcg acgacggcgc
      961 ggtacacgtc gtcgaactcg cgcggcacgg tgcccagcgc gcgcgcgaag gtcgcgccgc
     1021 cgttgacctc gctgcgcagg tgggcgacga ggtcgcgctg ggactgcacc tcggcctcgt
     1081 cggacagcgc cgtcagcgcg cgctccagcg gcaggccggc cgcgaccagg ccggcgagct
     1141 ggcgcgtcca caccgccagg cccgacgacg agaaggcctt gcgcgagaag cgcccggccg
     1201 gccggctgtc gtcggccacc gcgaccggcg cgagcccgag cggcacgagg ctcagcccgc
     1261 gcaggtgcgc gcgcgcggcc ttcatcgact cggcctcgac gatgccgttg gcggtcttgc
     1321 cggcgatgtc cagcgcttcg tacttgtagg cgggcatggc ggtgtcggta cgggaacgag
     1381 gttggggtcg gctggatcag tcgcgcgtca cgcgcacgac ctcctccagc gaggtcacgc
     1441 cctcggcgac gagccgctcg ccgtcctcgc gcatcgtgcg catgccgttg gcgcgggccg
     1501 cggcgatgat ctgctgctcg ggcgcgcggc cgtgcaccag cgcctggatc gcctcgtcga
     1561 ccaccatcag ctcgtacacg cccgtgcggc ccttgtagcc gctgtggccg cactgctcgc
     1621 agccgaccgg gtgccagcgg tgctcgtcgt cctcgcgctt gcagaactcg cacagcttgc
     1681 gcacgaggcg ctgcgcgagc acgcccagca gcgacgagct gagcaggaat ggctcgacgc
     1741 ccatgtcgat caggcgcgcg acggtcgacg gcgcgtcgtt ggtgtggacg gtggcgagca
     1801 ccaggtggcc cgtcagcgag gcctggatgg cgatctgcgc ggtctcgaag tcgcggatct
     1861 cgccgatcat caccacgtcc ggatcctggc gcaggatcgc gcgcagcgcc ttcgcgaagg
     1921 tgaggtcgat cttcgggttg acctgcgtct ggccgatgcc gggcagctcg tactcgaccg
     1981 gatcctccac cgtcaacacg ttggacgtgc gcgtatcgag gcgcgagagc gaggcgtaca
     2041 gcgtcgtcgt cttgccggag ccggtcgggc cggtcacgag cacgatgccg tgcggctggt
     2101 tgacgaggcg gttgaactcg tggagcacgt cgccgctcat gcccagcgcc tcgaggctga
     2161 acttcgcctc gcccttgtcg agcaggcgca gcaccgcgcg ctcgccgtgc gcggacggca
     2221 gcgtcgagac ccgcacgtcg atcgcgcggc cgccgatgcg cagcgagatc cggccgtcct
     2281 gcggcaggcg cttctcggag atgtcgagct cggccatgat cttcaggcgc gagatcagcg
     2341 ccgcgtgcag cgccttgttc ggctgcacga cctcgcgcag cgtcccgtcg acccggaagc
     2401 gcacgctgga gctgcgctcg tagggctcga tgtggatgtc gctcgcgccg tccttggccg
     2461 cctgcgtgag cagcgcgttg agcatgcgga tgatcggtgc gtcgttggcg gcctcgagca
     2521 ggtcctcgac ggcaggcagc tcctgcatca tgcgcgagag gtcgacggcg ctctcgacct
     2581 cgcccacgac cgccgcggcg ctgccctcgc cgcccgagta ggcctcggcg atgcgcgcgc
     2641 tcagcgtcgc gggcgccgag cgctcgatgc ggtcgatggc gtgcaggcgc agcacctcgg
     2701 ccagcgcggt gcgcgaggtg ccctcgctca cgtgcagcgt gcgctcgcgg ccgtcgtcct
     2761 cgagcagcat caggtgcgtc ttcgcccacg cgtagggcag gggatggcgt gcgcccatgg
     2821 tcgcctccgt ctcgttcgtc gcgcgtcgcg ggttacttcg tggggaccga cgcggccggc
     2881 gcgggtgcga cggtcgtcgt ggtcgtggtg gtcgtcgtcg acgagcccgg ctgcggcgcg
     2941 accggcgtca tctgccccag cggcacgaac gtgggctgcg gcgcctgctg cggcagcggc
     3001 tgggcagcgc cggggccgta gatcgcgccc gggcggtgca ggtcgggcag cggatcgacg
     3061 ttctgcatct tcacgctgcc ctcctgcgag tcctgcggcg tcggcaggac gcgggtgtcg
     3121 gcgccgatgc gcggcaccac cggcgcgtcg ttcacgcgca gcacgatgtt cttggcgggc
     3181 tgcgtggtct cctgctggcg gcggatcgcg tcgtagcggt tgagcgtgat cgcatcggcg
     3241 tccgattgcg agcgcatcac caccgggcgc aggaacagca tcaggttgcg cttcgagcgc
     3301 gtgcggttct ggctcttgaa caggttgccg atgaggggga tgttgcccag cagcggcacc
     3361 tggtcgacct cgtcgttgta gtcgtccgag atcaggccgc cgaggacgat cagcgagccg
     3421 tcgtcgacgg tcacgtagcc ttcgtacgag gtcttgttcg tcgtctggcc gccggtgccg
     3481 ggcgcgacgg aggagttctc ctcgtagatc gtcatgcgca cggtaccgtt ctccccgatc
     3541 gagctcttga tgcgcagcgt gatgccgacg tcctggcgct cgaccgtctg gaacgggttg
     3601 acggagctgg agctcgaccc cgtgttggtg tagctgccgg tgacgagcgg gatgttctgg
     3661 ccgacgacga tcttggcctc ctcgttgtcc atcgcgatca ggttcggcgt ggccaggacg
     3721 ttcgcgccgg tgttggtctc gaggaactgc gcgagcgcgc ccagcgtgta gtagctgccg
     3781 aggttcttga cgatgccgat gttcaggccg ttgctgatcg cgttggtgcc gctgccgacc
     3841 agcgtctgcg cggtcgagga gactccgctc gtgcccttga tcagcgacgc cgagccggtc
     3901 aggatgttgt tgagcgtgct ctgcgagccg aagttggtgc cggcgccggt gagcgtcgag
     3961 tcgcccttgc ggccgaacag gttctgccac tggaagccca tctgcgcggc cttggtctcg
     4021 tccatcgtga ccatcagcgc ctcgacgtag acctgcgcgc ggcgcacgtc gagctggtcg
     4081 atcaccgtcc gcatctgccg gtacagcggc tcggaggcgg tgatgatgag cgagttggtc
     4141 gcggggtcgg cctgcacctg cccgccggtg gacgggtgcg ccgaggccga caccgggttg
     4201 gtcgactgcg gcgaggcgtt gccgccggtc gtgccggagg tgctggtcat ggagctggag
     4261 ctcgtcgcgt cccggttgct cccgccgaac gcggaggtct ggccgctcgc gttgcccgag
     4321 ccgccgcccg tgccgccgct gctgccgccg ccgccgccca cgctgccgct ggcgatggcc
     4381 gcgcgcagca cctcggcgat cttggtcgcg tcggcgttct tcaggtacac cacccagatg
     4441 ttgccggccg ggccgccgcc ggcggtgggc tggtccagct tgtcgacgat catgcgcgcc
     4501 tgcgccatgc gcgtcgcatt ggccgcgcgg atgatcagcg agttggtgcg cgggtcgacg
     4561 aggatcgagg tctgcgccgt ctggcccggc acgcccggcg tctgcacggc gccgccgtcc
     4621 acgagcttct gcaccagctg cacgatgtcg gaggcgaccg cgttcttcag cggcacgacc
     4681 tcgagctcgg agcccgaggg ctggtcgagc gcctcgatga tgcggccgag gcgctggagg
     4741 ttgtcggcgt agtcggtgat gaccagcgag ttgctgcccg ggctcgcgtt gatcgtgttg
     4801 ttggcggtga tcagcggccg caggacggcc acgaggttgt tcgggttctc gtgctgcagc
     4861 ttgaagatct gcgtcagcac ctggtcgccc gagcgcgccg gggtacccac cgagacgacg
     4921 ccggtctgca gcttggcgtc ggcctcgggc accaccttca ggaacccgcc ggcctcgacg
     4981 acggtgaagc ccagcccgcg cagcgccgcg aggtagctgc gataggcctc cgacagcggc
     5041 tgcggcttgt cgttgtagac ggtgatcgtg cccttgacgc gcgggtcgac gaggatgtcg
     5101 cggttgagca tcgcgccgat cgcgcgcgag acggcctcga tgtcggcgtt gacgaagttc
     5161 agggtcaccg gcgtgttgcg cgcgcgggcg gtgggcacgg cgtcctcgcc ggccgaggcc
     5221 tgcgcggcgg cggcgtgggg cgcggcctgc acggg
//
LOCUS       DASVLN010000083         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_273588, whole genome shotgun sequence.
ACCESSION   DASVLN010000083 DASVLN010000000
VERSION     DASVLN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273588"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1315
                     /locus_tag="VF453_01805"
     CDS             161..1315
                     /locus_tag="VF453_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEX7686405.1"
                     /translation="MSSELVLSELRLQTRAEHDRLERILQLEEPMTLARYTAIVAGFD
                     AFLRAWEPRVQAALPPRLQPWFRARRRGGFATADLHWLRDVAGEAARAPDTSAVERLP
                     LDALAQVLGSIYVIEGSALGGRVIAPRLKATLDLVPGRGASYFHGFGGATGEMWRDFR
                     VLAALEVGESPAAIARACRSARRTFGVLIDLFAPLAREPDLRLPVELDREPEVIDVFT
                     AAPGDPGPDTIIDVLGPTEVSGELIDVTHDEQDTVRIARADLQIELPADPPAAAGPDT
                     VIDVLGSGEPADTMFDVLAEPREEGPVDAPPHADAADTVIDVLGSGEPPDTVFDVLAE
                     PREPGPVDAPPRDGDLDTIIDVLGSGEPPDTVIDVLTLHPAVDERRAPRR"
BASE COUNT          195 a          635 c          531 g          194 t
ORIGIN      
        1 gacgactgcg gcggacgcgc tccgccacgc agcgccgcgt ggcgtgcaat ccgcccgcaa
       61 gcgcgcccgc ccctgcactc ctcgcgggcg ccgccatccg ctaggatgtc cgtcgcccgc
      121 gccgtcaatc ccaagaaccg ccaagcccgc agtcgcgccc gtgagctccg aactcgtcct
      181 gtccgaactt cgcctgcaga cccgcgccga gcacgatcgc ctcgagcgca tcctgcagct
      241 cgaggagccg atgacgctgg cccgctacac ggcgatcgtc gccggcttcg acgccttcct
      301 gcgcgcctgg gagccccgcg tgcaggccgc gctgccgccg cgcctgcagc cgtggttccg
      361 cgcgcgccgg cgcggcggct tcgcgacggc cgacctccac tggctgcggg acgtcgccgg
      421 cgaggcggcg cgcgcgcccg acacgtcggc ggtcgagcgc ctgcccctgg acgcgctggc
      481 gcaggtgctg ggctcgatct acgtgatcga gggctccgcg ctcggcggcc gcgtcatcgc
      541 gccgcgcctc aaggcgacgc tggatctcgt gcccggccgc ggcgccagct atttccacgg
      601 cttcggcggc gcgaccggcg agatgtggcg cgacttccgc gtcctcgccg cgctggaggt
      661 cggggaatcc cccgccgcca tcgcccgcgc ctgccggagc gcgcggcgca cgttcggcgt
      721 gctgatcgac ctgttcgcgc cgctcgcgcg cgagccggac ctgcggctgc cggtcgagct
      781 cgatcgggag ccggaggtca tcgacgtctt caccgccgcg ccgggcgacc ccggcccgga
      841 caccatcatc gacgtcctcg gtccgaccga ggtctccggc gagctcatcg acgtcacgca
      901 cgacgagcag gacaccgtcc ggatcgcgcg cgccgacctg cagatcgagc tccccgccga
      961 tcctccggcc gcggcggggc ccgacacggt catcgacgtg ctgggctcgg gcgagccggc
     1021 cgacacgatg ttcgacgtcc tggccgagcc gcgcgaggaa ggccccgtcg acgccccgcc
     1081 gcatgcggac gcggcggaca ccgtgatcga cgtcctgggc tcgggcgagc cgccggacac
     1141 cgtgttcgat gtcctggccg agccgcgcga gccggggccg gtcgacgccc cgccccgcga
     1201 tggcgacctc gacaccatca tcgacgtgct cggctcgggc gagccgcccg acaccgtgat
     1261 cgacgtgctg accctgcacc cggcggtcga cgagcgtcgg gcgccgcggc gctgaaggcc
     1321 cgtcgcgccg cgcaggcgcc tcgattgctc gaagggcccg ccgcccgaaa tcgcgatgcg
     1381 atcgtcgcca tgcgaggacg catcacgtag actgcccctc cccagcccga cccccgcagc
     1441 ctgccctcat gcagaccgac cggcccgacc tcaccgcctg cgccgacgag ccgatccgga
     1501 ttcccggagc catccagccg cacggccgcc tgctcgtgtt cgacgaggac accgg
//
LOCUS       DASVLN010000084         6503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_434184, whole genome shotgun sequence.
ACCESSION   DASVLN010000084 DASVLN010000000
VERSION     DASVLN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434184"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..623)
                     /locus_tag="VF453_01810"
     CDS             complement(51..623)
                     /locus_tag="VF453_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7686406.1"
                     /translation="MNKLLAIAAAALAVAGTAHASTPVVVDALADAYGTSGATAVTLH
                     AGEAFTVTVDPTDLWSAGALPRWSNADGLTHDLYATGSDESGQPAGTLIGQNWGTYST
                     ADGTFAYGELVGRIGTGPYFAVGTSFSGVASSAGTLTLYYWDSYTLDNAGAVTAEVSA
                     VPEPGSAALMLAGLAAVGGLARRRAAKKSA"
     gene            992..1843
                     /locus_tag="VF453_01815"
     CDS             992..1843
                     /locus_tag="VF453_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7686407.1"
                     /translation="MQGSISRRPARQAGEDETASGLTSAVGVVEAIAAVAAVADGASA
                     GRLELEALESELVYRGPERRSDAYRGPERRGPGAGLAKLLASVLDEVDYGLLLIAADG
                     HVVHANHAARMELAESKSVRLQGRRLVGRSPADQRLLDDALVAARDGGRRKMLSLRNE
                     ESGAEGRSCDLSIIPLPTALSAHTSGHAVLIALPRPRIAETLSVDAYARELGLTRREQ
                     EVLAGLCEGRRVKEIAARLEIGEETVRSHVKRLKAKTGCTGIVDIVNQLSRLPPMVGA
                     LRQSTAA"
     regulatory      1909..1995
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            2091..3524
                     /gene="ahcY"
                     /locus_tag="VF453_01820"
     CDS             2091..3524
                     /gene="ahcY"
                     /locus_tag="VF453_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX7686408.1"
                     /translation="MNAALKPLPTDAYVVADLSLADWGRKEIAIAESEMPALMAVRSE
                     YGASQPLKGARIAGSLHMTIQTAVLVETLQALGAEVRWASCNIFSTQDHAAAALVAAG
                     TPVFAYKGETLKDYWDYTHRIFEFGAAGAAGEGPNMILDDGGDATLLMHLGQRAEKDA
                     SVVANPTSEEERELFAAIRAKLAVDPGWYTRKSREIVGVTEETTTGVHRLAEMSAKGT
                     LLFRAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVAVVAGYGDVGKGSAQ
                     ALRALSAQVWVTEIDPINALQAAMEGYRVVTMDEAAALGDIFVTCTGNKNVITYDHMN
                     AMKEQAIVCNIGHFDNEIDIASLEHCKWEEIKPQVDHVIFPNGKRIIMLAKGRLVNLG
                     CATGHPSYVMSSSFANQTLAQIELFSRPDQYQVGQVYVLPKHLDEKVARLQLSKLNAK
                     LTELTDEQAAYIGVSKNGPYKPDTYRY"
     gene            3743..4573
                     /gene="metF"
                     /locus_tag="VF453_01825"
     CDS             3743..4573
                     /gene="metF"
                     /locus_tag="VF453_01825"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX7686409.1"
                     /translation="MRTIPLSLEFFPPKTPEGVTKLAAARAELYALEPEFCSVTYGAG
                     GSNQQGTLAAVQAIRAEGRVGVPHFTCIGTTRASVGETLDAFRAAGIDRIVALRGDLP
                     SGAGSFGEFRYASELIEQIRAHSGDHFHIEVAAYPETHPQARSPEADLKAFAAKVAAG
                     ADSAITQMFFNADAYFRFVEDARRIGVDVPIVPGIMPITNSSGLMRFADATGCEIPRW
                     MRLRLQAFGDDTKGIRAFGLEVMAALCERLVAGGAPGLHFYTMNQSAATLALARALGR
                     "
     gene            4807..>6503
                     /locus_tag="VF453_01830"
     CDS             4807..>6503
                     /locus_tag="VF453_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7686410.1"
                     /translation="MPTFPKSAAAPAFALLPLVAALAAAPAAAANFTITGSSTSAQTL
                     GSGSGQAGTVTAGSSLTVGGGTVAVSVTGNNATLTNLGTISQTRSGRVIRDNTGVSGL
                     VVTNGSATNSTALMQAADADVIQMNKSPAGVTLDNYGQMISLNASAGGAQAVDFNAIA
                     SGPNAVNNYAGALMKAFEADAVRPGVNGSVYNAGTILSITTTGSSSDGIDMQNNSGVS
                     IVNDTTGLIEGGRHGITGGAVDATVAYLASITNKAGGVIQGDNGSGINIDGFNAKELV
                     TVVNAGTIKGYGVTGDGDGVDVDGLVNLTNTGVIRSGNAVPDAGDAIAYSEGVSVGGG
                     TITNSGTIEGLVAAGNAKAVGRGITLTGNDITSGPLAGTREGLYGNAVVDNLAGGLIR
                     GQNDSAIAVTGAKSGYTVTINNAAGGTIQGGGTTAPAILGGLDDDTVNDSGRIDGAGS
                     GKAIDLGGGNNTVNILGGHAVVLGDIDGGAGGTNALHFTIGAGNAFSYAGAISDFATV
                     DVRSGMVTLSGASTYTGTTTVESGATLVLAGANRLAAGSALDLAGGTLRLAGVTGADA
                     "
BASE COUNT         1036 a         2354 c         2281 g          832 t
ORIGIN      
        1 ccaatgaaaa cgggcgccga ggcgcccgtc gtccgttcgc ggaggccggc tcaggccgac
       61 ttcttcgcgg cgcggcggcg cgccaggccg ccgacggccg cgaggccggc cagcatcagg
      121 gccgcggaac cgggttccgg cacggccgag acttcggcgg tcaccgcacc ggcattgtcg
      181 agcgtgtagc tgtcccagta gtacagggtc agcgtgccgg cgctgctggc gacgccgctg
      241 aagctggttc cgaccgcgaa gtacgggccc gtgccgatgc ggccgaccag ttcgccgtac
      301 gcgaacgtgc cgtcggcggt ggagtaggtg ccccagttct ggccgatgag cgtgccggcg
      361 ggctggccgg actcgtcgga gcccgtggcg tacaagtcgt gcgtgaggcc gtcggcgttg
      421 gaccagcgcg gcagcgcacc ggcgctccac aggtcggtcg ggtcgacggt caccgtgaag
      481 gcctcgccgg cgtgcagggt gacggccgtg gcgccggacg tgccgtaggc gtcggccagg
      541 gcgtcgacga ccaccggggt cgaggcgtgc gcggtgccgg cgacggcgag cgcggcggca
      601 gcgatggcga gcagcttgtt catcgtggga accctcctgg acatgcgccg ggcggacgcc
      661 cgagctcgac agtgattggg cgccgttttg ctgctgtgca ccatccccaa agctggggat
      721 ccggtgcatg cccccggccg tccgtcgcgc tgccggcgcc atgcatcgga aacgcaaagc
      781 cccagctcga ccgtggcggg gatactgctc gacaatgagg gcgggcgcgg ccatcgcagg
      841 ccgaaatagt gcaggacttg gctaagcgcg cgcttttgac gccgggcggc cttgttcccc
      901 cgccggcaaa acaagataaa cgcgctgtcg acccgccggc gtcttcgccg cgagccgcgt
      961 cacgaacaaa catcagtcga gggttttcga catgcagggc agcatcagca ggcgtccggc
     1021 ccgccaggcc ggcgaagacg agaccgcatc ggggttgacg agcgctgtcg gcgtcgtcga
     1081 ggccatcgcg gccgtcgccg cggtcgccga cggcgcctcg gccggccggc tcgagctcga
     1141 ggcgctcgag tcggagctcg tctaccgcgg tcccgagcgc cgcagcgacg cctaccgcgg
     1201 ccccgagcgc cgcggcccgg gcgccgggct ggccaagctg ctggccagcg tgctcgacga
     1261 ggtggactac ggcctgctgc tgatcgccgc cgacggccac gtcgtgcacg ccaaccacgc
     1321 ggcgcgcatg gagctcgcgg aatcgaagtc ggttcgcctg cagggccgcc ggctcgtcgg
     1381 ccgctcgccc gccgaccagc ggctgctcga cgacgcgctc gtcgcggcgc gcgacggcgg
     1441 gcggcgcaag atgctcagcc tgcgcaacga ggagtcgggc gcggagggcc gctcgtgcga
     1501 cctgtccatc atcccgctgc cgaccgcgct gtccgcgcac acgagcggcc acgcggtgct
     1561 gatcgcgctg ccgcgacccc gcatcgccga gacgctgtcc gtcgacgcgt acgcacgcga
     1621 gctcggcctc acgcggcgcg agcaggaggt gctcgccggg ctgtgcgagg gccggcgcgt
     1681 caaggagatc gcggcccgcc tcgagatcgg cgaggagacc gtgcgcagcc acgtcaagcg
     1741 cctgaaggcc aagaccggct gcaccggcat cgtcgacatc gtcaaccagc tctcgcgcct
     1801 gccgcccatg gtcggtgcgc tgcgccagtc cacggccgcc tgaggccggg cggctggacg
     1861 cgaaaagtcc tctcgccgcc cgcgcgccgg ccgatataat cgccaaccat tcccgaggag
     1921 cgttgcgagg cccggctgcc cagccgatgc gccccaggct cgggaccctt cccaaggcaa
     1981 cggcgctcac ccgcttcgcg accagcgaac gttccgggtg agctgcatct cgtcgcagcg
     2041 aactgggcga gctcgccggc cgcgtccgcg ggtcttccac ggagatcccc atgaacgctg
     2101 cactcaagcc cctgcccacc gacgcctacg tcgtcgccga cctctcgctg gccgactggg
     2161 gccgcaagga aatcgccatc gccgagagcg agatgcccgc gctgatggcc gtgcgctcgg
     2221 aatacggcgc gagccagccg ctcaagggcg cgcgcatcgc cggcagcctg cacatgacca
     2281 tccagaccgc ggtgctggtc gagacgctgc aggccctcgg cgccgaggtg cgctgggcgt
     2341 cgtgcaacat cttctcgacg caggaccacg ccgccgccgc gctggtcgcc gccggcacgc
     2401 cggtcttcgc gtacaagggc gagacgctca aggactactg ggactacacc caccggatct
     2461 tcgagttcgg cgccgcgggc gcggccggcg agggcccgaa catgatcctc gacgacggcg
     2521 gcgacgcgac gctgctgatg cacctgggcc agcgcgccga gaaggacgca tcggtcgtcg
     2581 ccaacccgac cagcgaggag gagcgcgagc tcttcgccgc gatcagggcc aagctggccg
     2641 tcgacccggg ctggtacacg cgcaagagcc gcgagatcgt gggcgtcacc gaggagacga
     2701 cgaccggcgt gcaccgcctg gccgagatgt ccgccaaggg cacgctgctg ttccgcgcga
     2761 tcaacgtcaa cgactcggtc accaagagca agttcgacaa cctctacggc tgccgcgaat
     2821 cgctggtcga cggcatcaag cgcgccaccg acgtgatgat cgccggcaag gtcgcggtcg
     2881 tggccggcta cggcgacgtc ggcaagggct cggcgcaggc gctgcgcgcg ctgtcggcgc
     2941 aggtctgggt caccgagatc gacccgatca acgcgctgca ggcggcgatg gagggctacc
     3001 gcgtcgtcac gatggacgag gccgcggcgc tgggcgacat cttcgtcacc tgcaccggca
     3061 acaagaacgt catcacctac gaccacatga acgccatgaa ggagcaggcg atcgtgtgca
     3121 acatcggcca cttcgacaac gagatcgaca tcgcctccct cgagcactgc aagtgggagg
     3181 agatcaagcc gcaggtcgac cacgtgatct tcccgaacgg caagcgcatc atcatgctcg
     3241 ccaagggccg gctcgtgaac ctgggctgcg ccaccggcca tccgagctac gtgatgtcgt
     3301 cgagcttcgc caaccagacg ctggcgcaga tcgagctctt cagccgtccc gaccagtacc
     3361 aggtcgggca ggtctacgtg ctgcccaagc acctcgacga gaaggtcgcg cggctgcagc
     3421 tgtccaagct caacgcgaag ctgaccgagc tgaccgacga gcaggccgcg tacatcggcg
     3481 tgtccaagaa cggcccctac aagcccgaca cttacaggta ctgactcgct agcactccct
     3541 gccccccaag ggggtgagcc tggacacagg ccgtccggtg gacggactgt cgcctggcga
     3601 acgccggacg cttgcgtccg gcaggcgtgc agaccggcaa agccggatct gcggcttccc
     3661 cgtgtctcgt cggatgcggg cgagcggcag cgccgatgtc ggcctgcacc gtcgctgcgc
     3721 tcgtgacgat ggaagaattt ctatgagaac catacccctg agcctcgagt tcttcccgcc
     3781 gaagacgccc gaaggcgtga ccaagctggc ggccgcgcgg gccgagctgt acgcgctgga
     3841 gccggagttc tgttccgtca cctacggcgc gggcggatcg aaccagcagg gcacgcttgc
     3901 ggcggtgcag gcgatccgcg cggaaggccg ggtcggcgtg ccgcacttca cctgcatcgg
     3961 caccacgcgc gcgagcgtgg gcgagacgct ggacgccttc cgggcggccg gcatcgaccg
     4021 catcgtcgcg ctgcgcggcg acctgcccag cggcgcgggc agcttcggcg agttccgcta
     4081 cgccagcgaa ctgatcgagc agatccgcgc ccactccggc gaccacttcc acatcgaggt
     4141 cgccgcctat ccagagaccc atccgcaggc ccggtcgccc gaggccgacc tgaaggcctt
     4201 cgccgccaag gtcgccgcgg gcgcggactc ggcgatcacg cagatgttct tcaacgcgga
     4261 cgcctacttc cgcttcgtcg aggacgcacg ccgcatcggc gtcgacgtgc ccatcgtgcc
     4321 gggcatcatg ccgatcacca actccagcgg cctcatgcgc ttcgccgacg ccaccggctg
     4381 cgagatcccg cgatggatgc gcctgcgcct gcaggccttc ggcgacgaca cgaagggcat
     4441 ccgggcgttc ggcctcgagg tgatggccgc gctgtgcgag cgcctcgtgg ccggcggcgc
     4501 gccggggctg cacttctaca cgatgaacca gtcggcggcg acgctggccc tggcccgggc
     4561 gctcggtcgc tgagccctgc gcggggcggg cgtcgcgccc gccttccggg ccccgcggga
     4621 tgcggcggcc cgggtttcct ctcgcccggc cgtcacggca ccgtcacgcg cggcgcggac
     4681 acttccgcgc cgcgggagcg gccttcgcat gcatgcgtgc acctggaagc cgcccccgcc
     4741 ttttcatgcc ggggcgtcgt ggaggcgtcg ccggcaccgt cgttggatac aagaaccgga
     4801 ccgcccatgc cgaccttccc gaagagcgcc gccgcgcccg cattcgccct gctgccgctc
     4861 gtcgcggccc tcgccgccgc gccggccgcc gccgcgaact tcacgatcac cggctcgtcg
     4921 acgagcgcgc agacgctggg cagcggctcc ggccaggccg gcacggtgac ggccggctcg
     4981 tcgctgaccg tcggcggcgg cacggtggcg gtctcggtca ccggcaacaa cgccacgctg
     5041 accaacctcg gcacgatcag ccagacgcgc agcggccgcg tgatccgcga caacaccggc
     5101 gtcagcggcc tcgtggtcac caacggcagc gcgacgaact cgacggccct gatgcaggcc
     5161 gccgacgcgg acgtgatcca gatgaacaag tcgccggccg gcgtgacgct cgacaactac
     5221 ggccagatga tctcgctgaa cgcgtcggcg ggcggcgcgc aggcggtgga cttcaacgcg
     5281 atcgcctccg gcccgaacgc ggtcaacaac tacgccggcg cgctgatgaa ggccttcgag
     5341 gccgacgccg tgcggcccgg cgtcaacggc agcgtctaca acgccggcac catcctgtcg
     5401 atcacgacga ccggcagctc gagcgacggc atcgacatgc agaacaactc gggcgtgtcc
     5461 atcgtcaacg acacgaccgg cctgatcgag ggcggccgcc acggcatcac cggcggcgcg
     5521 gtcgacgcga ccgtcgccta cctggcctcg atcaccaaca aggccggcgg cgtgatccag
     5581 ggcgacaacg gctcgggcat caacatcgac ggcttcaacg ccaaggagct cgtgaccgtc
     5641 gtgaacgccg gcacgatcaa gggctacggc gtgacgggcg acggcgacgg ggtggacgtc
     5701 gacgggctcg tcaacctgac caacaccggc gtgatccgct cgggcaacgc ggtgccggac
     5761 gcgggcgacg cgatcgccta cagcgagggc gtcagcgtcg gcggcggcac gatcaccaac
     5821 tcgggcacga tcgagggcct ggtcgccgcg ggcaacgcga aggcggtcgg ccgcggcatc
     5881 acgctgacgg gcaacgacat cacttcgggc ccgctcgccg gcacgcgcga gggcctgtac
     5941 ggcaacgcgg tcgtcgacaa cctcgccggc ggcctgatcc gcggccagaa cgactcggcc
     6001 atcgccgtca ccggcgcgaa gagcggctac accgtgacga tcaacaacgc ggccggcggc
     6061 acgatccagg gcggcggcac gaccgcgccg gccatcctcg gcgggctgga cgacgacacg
     6121 gtcaacgaca gcggccgcat cgacggcgcc ggcagcggca aggcgatcga cctgggcggc
     6181 ggcaacaaca cggtcaacat cctgggcggc cacgcggtcg tgctgggaga catcgacggc
     6241 ggcgccggcg gcacgaacgc gctgcacttc acgatcggcg cgggcaacgc gttctcgtac
     6301 gcgggcgcga tctcggactt cgccacggtc gatgtgcgca gcggcatggt gacgctgtcg
     6361 ggcgcgagca cctacaccgg cacgacgacg gtcgagtccg gcgcgacgct ggtgctcgcc
     6421 ggcgccaacc gcctcgcagc cggcagcgcg ctcgacctcg cgggcggcac gctgcggctg
     6481 gcgggcgtga cgggcgccga cgc
//
LOCUS       DASVLN010000085         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_218751, whole genome shotgun sequence.
ACCESSION   DASVLN010000085 DASVLN010000000
VERSION     DASVLN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218751"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VF453_01835"
     CDS             <1..1082
                     /locus_tag="VF453_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7686411.1"
                     /translation="FQMGMPACDAFPRKLWATLGVRRLRAAGVADLAYPDPRGSPRLR
                     QAIAAYLLVSRGVDCRPEQVFVTAGYRASLDLVTRVLLRPSDRVCIEDPYYPATLALL
                     RAAGLRVACVAVDEEGLRVDRLVARHADARAVVVTPAHQAPLGVSMTLARRMKLLAWA
                     AANGAWVVEDDYDSEFRYVGAPLPALKSADGAGRVLYAGTFSKVLHPGLGLAYLVVPT
                     SQVDAFTQQVVRTGNGCAHLQQEIVADFMELGHFPRHIRRMRGLYAKRRRWLVEALHA
                     AFGRGVQVDLQAGGMHLLARFPGHGADDALAERAQARGLAVQALSRRSSGTHAGQGLL
                     MGFTNVVSREDAAALAGKLRAAMGR"
     gene            1195..1653
                     /locus_tag="VF453_01840"
     CDS             1195..1653
                     /locus_tag="VF453_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7686412.1"
                     /translation="MTDIIHRIGIKAPAADVHAALSTIPGLAGWWTEQTSGSSRVGEL
                     VAFRFHLEDGSLLGGFDMEVTELSPEDKVRWRVKDGPPEWIGTDIEFSLLRQEDWTVV
                     MFGHRGWREEVEFTAHCSTKWATFLVSLRDFVETGKGRPAPNDVRIGDWH"
BASE COUNT          276 a          748 c          738 g          291 t
ORIGIN      
        1 cgttccagat gggcatgccg gcctgcgacg cgttcccgcg caagctctgg gcgaccctgg
       61 gcgtgcgccg gctgagggcc gcgggcgtgg ccgatctcgc atatcccgat ccgcgcggct
      121 cgccgcgcct gcgccaggcg atcgcggcct acctgctggt gtcgcgcggc gtcgactgcc
      181 ggcccgagca ggtcttcgtc accgccggct accgcgccag cctcgacctc gtgacgcgcg
      241 tcctgctgcg gcctagcgac cgggtctgca tcgaggaccc gtactacccg gccacgctcg
      301 cgctcctgcg cgccgccggc ctgcgcgtcg cctgcgtggc ggtggacgag gagggcctgc
      361 gcgtcgacag gctcgtcgcg cggcacgccg acgcgcgcgc cgtcgtggtg acgcccgcgc
      421 accaggcgcc gctgggcgtg tcgatgacgc tcgcgcgacg gatgaagctg ctcgcctggg
      481 cagcggcgaa cggcgcatgg gtggtcgagg acgactacga cagcgagttc cgctacgtcg
      541 gcgcgccgct gcccgcgctg aagagcgccg acggcgccgg acgcgtgctg tacgccggca
      601 ccttcagcaa ggtgctgcat ccgggactcg gcctcgcata cctggtcgtg ccgacgtcgc
      661 aggtcgatgc cttcacgcag caggtcgtgc gcaccggcaa cggatgcgcg cacctgcagc
      721 aggagatcgt cgcggacttc atggaactcg gccacttccc gcgccacatc cgccggatgc
      781 gggggctgta cgcgaagcgg cggcggtggc tggtcgaggc gctgcatgcc gccttcggcc
      841 gcggcgtgca ggtcgacctg caggcggggg gcatgcacct gctggcgcgc tttcccggtc
      901 atggcgccga cgacgcgctg gcggagcgcg cccaggctcg cgggctggcc gtgcaggcgc
      961 tgtcgcgccg atcctccggc acgcatgccg ggcagggcct gctgatggga ttcaccaacg
     1021 tcgtttcccg ggaggacgcg gccgccctcg cggggaagct gcgggcggcg atggggcgct
     1081 gattcgcgac agccttgtta ctcacgctcc ttgcggccga tcgccgacat tggcggtgac
     1141 gacgcaccga tcgcggccgt cgcgccccct caacgcatcg atcggagtcc ccgcatgacc
     1201 gacatcatcc atcgcatcgg catcaaggcg cccgccgccg acgtccacgc cgcgctctcg
     1261 accatccccg gcctcgcggg ctggtggacc gagcagacct cgggcagctc gcgcgtcggc
     1321 gagctcgtcg cgttccgctt ccacctcgag gacggcagcc tgctcggcgg cttcgacatg
     1381 gaggtgaccg agctgtcccc cgaggacaag gtgcgctggc gcgtcaagga tggcccgccg
     1441 gagtggatcg gcaccgacat cgaattcagc ctcttgcgcc aggaggactg gaccgtcgtg
     1501 atgttcggcc accgcggctg gcgcgaggag gtcgagttca cggcccactg cagcaccaag
     1561 tgggcgacct tcctcgtcag cctgcgcgac ttcgtcgaga cgggcaaggg ccggccggcg
     1621 cccaacgacg ttcggatcgg ggactggcat tgacgtcgag tgcggggcgc ccgggtcggc
     1681 gcttcgatga cgcggtcttc atacagatag ggggttaggg tatcggagga tgcggtgcct
     1741 gcgcggcggg gccgtgcgac tcgcgacgcc gccgcgcgac ggcgccgtcg agcggcgcgg
     1801 acgccactac gagaattcgt cgtcatcgaa cgatcgcaaa cgatcccgat gatcgccgcc
     1861 accgtagccc ccgcgcgaat gccgcgcggg tcggcgcccc gcaacactcg acggcaccgc
     1921 tcgatggcca ccagctccct gtccggattc agcttcaacc ccgccgcgac gttcctgccc
     1981 gacaccgccg cccagcaggc cgccgccgag cgccagcggg agatcgcggc cgcgcaggag
     2041 gcggcccggc agg
//
LOCUS       DASVLN010000086         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_273633, whole genome shotgun sequence.
ACCESSION   DASVLN010000086 DASVLN010000000
VERSION     DASVLN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273633"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /gene="murD"
                     /locus_tag="VF453_01845"
     CDS             <1..1337
                     /gene="murD"
                     /locus_tag="VF453_01845"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX7686413.1"
                     /translation="ADAAQAAEQAAAAAQRAAAEPVAAADTMSEDACASIEEPPTDDD
                     ADLPALAALDGVDPESLADADAEAPADDASEAAPAAPDDEAPPIALIPPPPPEPVFSH
                     LPQVWVLELSSFQLDGVRHFAPSAAVVLNVTQDHLDWHGSMAAYAKAKAQVYGLEPGA
                     ETILVVNRDDPAVEAMVPEPVVIKPAARGQRSRTVARRVVRFGLDAPRAPGDFGRVEE
                     NGMAWLVRAMEVDPTIKRRPGEVDDIVLNRLMPADALRIRGRHNASNALAALALCTAI
                     GLPLAPLLHGLREYRGEPHRVEPVGQVGDVEFFDDSKGTNVGATVAALAGLGLDRAPH
                     KLVVILGGDGKGQDFAPLAEPLRRHARAAATIGKDAPALEAVLATASVPFERHASLQE
                     ATRWALAQAQPGDAVLLSPACASLDMFRDYKHRADVFCETLRELAADEGDVA"
     gene            1334..2617
                     /gene="ftsW"
                     /locus_tag="VF453_01850"
     CDS             1334..2617
                     /gene="ftsW"
                     /locus_tag="VF453_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX7686414.1"
                     /translation="MTGVFSNLRQHLPEWPGFRPVEWLRKRRDAGSADERVPIRDWVD
                     AGRPTRVMGVDSALIWVIAALLAFGLVMVYSASVALPDSPRFALYSSTHFLSRQASFI
                     VIGAVCAFATAQVPIAVWEKWAPWIFALAMVMLALVLIPHLGKGVNGARRWIPLGVLN
                     FQPSELAKLGMCMYAASYMVRKMDVKEHFVKAVWPMAVALAVIGVLLLAEPDMGAFMV
                     IAAIALGVLFLGGVNGRMFLLSVAVLLGAFVLMIVFSEFRRQRIFAYLDPWNPTYAQG
                     KAYQLTHSLIAFGRGEIFGQGLGSSIEKLHYLPEAHTDFLMAVIGEELGFVGVFIVIA
                     AFFWLTRRLFIIGRQAIALDRVFAGLMCQGVGLWMGAQSFINFGVNLGVLPTKGLTLP
                     LLSYGGSGVVLNLVALALVLRVDMESRALMRGGRA"
     gene            2614..>3641
                     /gene="murG"
                     /locus_tag="VF453_01855"
     CDS             2614..>3641
                     /gene="murG"
                     /locus_tag="VF453_01855"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX7686415.1"
                     /translation="MTTTTDVATRVPPARPVGTRHLVVMAAGTGGHVMPGLAVAREVQ
                     ARGWTVSWLGTRTGMENRLVPPSGIPLDTIDFAGVRGKGLMGNLRGLLKLLAAFGACR
                     FILSSRKATAVLGMGGYVCYPGGKAARRLGKPLVLVNADAALLMSNKALLATCDRIAF
                     GFDGEDARRTNKSVVTGNPVRAEIEAIAPPAERFATRHGPLRVLLVGGSLGAKALNEA
                     LPAALQMIPAGQRPRVTHQTGEKHWAAVQADYAARGVEAEVVPFIDDMPRRLAECDLV
                     VCRAGAITVSELCAAGVASILVPLVISTTSHQRDNAEYLAGRGAARHLPQSELTPERL
                     AAELRAATR"
BASE COUNT          461 a         1362 c         1301 g          517 t
ORIGIN      
        1 ccgccgacgc cgcccaggcc gccgaacagg ccgcggccgc agcgcagcgt gccgctgccg
       61 aaccggtcgc tgcagcggac acgatgtccg aagacgcctg cgcaagcatc gaggagccgc
      121 cgaccgacga tgacgccgac ctgcccgcgc tcgcggcgct cgacggcgtc gatcccgaga
      181 gcctcgccga cgccgacgcc gaagcgcctg ccgacgatgc ctccgaggcc gcgcccgccg
      241 cgcccgacga cgaggcgccg ccgatcgccc tgatcccgcc gccgccgccc gagccggtct
      301 tcagccacct gccgcaggtc tgggtgctgg agctctccag cttccagctc gacggcgtgc
      361 gccacttcgc gccgagcgcc gcggtcgtcc tcaacgtgac gcaggaccac ctcgactggc
      421 acggctcgat ggccgcctac gcgaaggcga aggcgcaggt ctacggcctg gagccgggcg
      481 ccgagaccat cctcgtcgtc aaccgcgacg atccggccgt cgaggcaatg gtgcccgagc
      541 ccgtcgtgat caagcccgcc gcgcgcggcc agcgcagccg caccgtcgcg cgccgcgtcg
      601 tgcgcttcgg cctggacgcg ccgcgcgcgc cgggcgactt cgggcgggtc gaggagaacg
      661 gcatggcctg gctggtgcgc gcgatggagg tcgacccgac gatcaagcgc cggcccggcg
      721 aggtggacga catcgtgctg aaccgcctga tgccggccga cgcgctacgc atccgcggcc
      781 gccacaacgc ctccaacgcg ctcgcggcgc tcgcgctgtg caccgcgatc ggcctgccgc
      841 tcgcgccgct gctgcacggt ctgcgcgagt accgcggcga gccgcaccgc gtcgagcccg
      901 tcggccaggt cggcgacgtc gagttcttcg acgattcgaa gggtaccaac gtcggcgcga
      961 ccgtcgctgc cctggcgggc ctcgggctcg accgcgcgcc gcacaagctg gtcgtgatcc
     1021 tcggcggcga cggcaagggc caggacttcg cgccgctggc cgagccgctg cgccgccacg
     1081 cgcgcgcggc cgcgacgatc ggcaaggacg cgcccgccct cgaggccgtg ctggcgaccg
     1141 cgagcgtccc gttcgagcgc cacgcgtcgc tgcaggaggc gacgcgctgg gcgctcgcgc
     1201 aggcgcagcc cggcgacgcc gtcctgctga gcccggcctg cgcgagcctg gacatgttcc
     1261 gcgactacaa gcaccgcgcc gacgtgttct gcgagacgct gcgcgagctg gccgcggacg
     1321 aaggggacgt ggcatgaccg gcgtcttctc gaacctccgc cagcacctgc ccgagtggcc
     1381 cggcttccgc ccggtcgagt ggctgcgcaa gcgccgggac gccgggtcgg ccgacgagcg
     1441 cgtgccgatc cgcgactggg tcgacgccgg ccgccccacg cgcgtgatgg gcgtggactc
     1501 ggcgctgatc tgggtgatcg cggcgctgct ggccttcggc ctcgtgatgg tctattcggc
     1561 ctcggtcgcg ctgccggaca gcccgcgctt cgcgctgtat tcgtcgaccc acttcctctc
     1621 gcgccaggcc tcgttcatcg tcatcggcgc cgtgtgcgcg ttcgccaccg cgcaggtccc
     1681 gatcgcagtc tgggagaagt gggcgccgtg gatcttcgcg ctcgcgatgg tgatgctcgc
     1741 gctggtgctg atcccgcacc tgggcaaggg cgtcaacggc gcgcgccgct ggatcccgtt
     1801 gggcgtgctg aacttccagc ccagcgagct cgccaagctg ggcatgtgca tgtacgcggc
     1861 cagctacatg gtgcgcaaga tggacgtcaa ggagcacttc gtgaaggccg tctggccgat
     1921 ggccgtggcg ctggccgtca tcggcgtgct gctgctggcg gagcccgaca tgggcgcgtt
     1981 catggtgatc gcggcgatcg cgctcggcgt cctgttcctc ggcggcgtca acggccgcat
     2041 gttcctgctc agcgtcgcgg tgctgctggg cgcgttcgtg ctgatgatcg tgttcagcga
     2101 gttccgccgc cagcgcatct tcgcgtacct cgacccgtgg aacccgacct acgcgcaggg
     2161 caaggcctac cagctgacgc actccctgat cgcgttcggc cgcggcgaga tcttcggcca
     2221 gggcctcggc tcgtcgatcg agaagctgca ctacctgccc gaggcgcaca ccgacttcct
     2281 gatggccgtg atcggcgagg agctgggctt cgtcggcgtg ttcatcgtga tcgcggcgtt
     2341 cttctggctg acgcgccggc tgttcatcat cggccggcag gcgatcgcgc tcgatcgcgt
     2401 cttcgccggc ctgatgtgcc agggcgtggg cctgtggatg ggcgcgcagt ccttcatcaa
     2461 cttcggcgtc aacctcggcg tgctgccgac caagggcctg accctgcccc tgctgagcta
     2521 cggcggctcg ggggtggtgc tcaacctcgt cgccctcgcg ctggtgctgc gcgtcgacat
     2581 ggaaagccgc gcgctgatgc ggggaggtcg cgcatgacga ccacgaccga cgtcgcgacc
     2641 cgcgtgccgc ccgcgcgccc cgtgggcacg cgccacctcg tggtgatggc ggccggcacc
     2701 ggcggccacg tgatgccggg cctcgcggtc gcgcgcgagg tgcaggcgcg cggctggacg
     2761 gtcagctggc tcgggacccg cacgggcatg gagaaccgcc tcgtgccgcc gtcgggcatc
     2821 ccgctcgaca cgatcgactt cgcgggcgtg cgcggcaagg gcctgatggg caacctgcgc
     2881 ggcctcctca agctcctcgc ggcgttcggc gcctgccgct tcatcctgag ttcgcgcaag
     2941 gcgaccgcgg tgctggggat gggcggctac gtctgctatc ccggcggcaa ggcggcgcgc
     3001 cggctcggca agccgctggt gctcgtcaac gcggacgccg cgctcctgat gtccaacaag
     3061 gcgctcctcg cgacctgcga ccgcatcgcc ttcggcttcg acggcgagga cgcgcgccgc
     3121 accaacaaga gcgtggtgac gggcaacccg gtgcgtgccg agatcgaggc gatcgcgccg
     3181 ccggccgagc gcttcgccac ccgccacggc ccgctgcgcg tgctgctggt gggcggcagc
     3241 ctgggcgcga aggcgctcaa cgaggcgctg cccgccgcgc tgcagatgat ccccgccggc
     3301 cagcgcccgc gcgtcacgca ccagaccggc gagaagcact gggccgcggt gcaggccgac
     3361 tacgcggcgc gcggcgtcga ggccgaggtc gtgcccttca tcgacgacat gccgcgtcgc
     3421 ctggccgagt gcgacctcgt cgtctgccgc gccggcgcga tcacggtcag cgagctctgc
     3481 gccgcgggcg tcgcgagcat cctcgtgccg ctcgtgatca gcacgacctc gcaccagcgc
     3541 gacaacgccg agtacctggc cggccgcggc gccgcgcgcc acctgccgca gtccgagctg
     3601 acgcccgagc gcctggccgc cgagctgcgc gccgcgacgc g
//
LOCUS       DASVLN010000087         4686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_434285, whole genome shotgun sequence.
ACCESSION   DASVLN010000087 DASVLN010000000
VERSION     DASVLN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434285"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VF453_01860"
     CDS             complement(<1..619)
                     /locus_tag="VF453_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX7686416.1"
                     /translation="MNTVLGIDLGTSELKLALLGEDGRLLATAGAPLAVSRPRPHWSE
                     QHPQDWWDALVAAAATLRAAHPAEFAAVQAIGLAGQMHGAVLLDAAGMVLRPAILWND
                     TRSGAECDELVRRMPALPAIAGNLAMPGFTAPKLLWIERHEPDVHARVARVLLPKDWL
                     RWRLTGSAFSDMSDAAGTLWLDVGARAWSDALLDAGGMRRAQMPEL"
     gene            complement(616..1392)
                     /locus_tag="VF453_01865"
     CDS             complement(616..1392)
                     /locus_tag="VF453_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7686417.1"
                     /translation="MTTSALIEFAGVGKSFGGVHAVEDVSLALDPGEVVAVLGHNGAG
                     KSTLMKMLAGAFAVDRGEIRVGGQAARIEKPADAQALGIETIHQTLALADNLDAVANL
                     FLGREIMTRWNTLDDYAMEAAARRVFAQLNPNFRNIRVPVRALSGGQRQVVAISRALH
                     FNARVLVMDEPCAALGPEETRMVHELVKRLKAQGVGIFLITHDMPDVFGLSDRLAVMK
                     NGRMVGTYRTASLSEDDVLGMIIGGKPVRHVAEVHRGVSA"
     gene            complement(1389..2567)
                     /locus_tag="VF453_01870"
     CDS             complement(1389..2567)
                     /locus_tag="VF453_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7686418.1"
                     /translation="MESLQLSALDLRLALMAAVLAAMAVVFHVLTGNFLTPENLYNIA
                     QQTAVVGIVATAIALVVVGRHIDLSVGSVMGFVGVLIAWLQYTHGWSWMAACGVGLLA
                     AIAVGLYQGGLTAYLGVPSFVVTLGGLMSFRGAAFLVADGKTQPVTDPVFLWFGGGLD
                     GALGIVGSWALAAVLAALLAVRIARMRRARARHGFPLRPLWADLGFTLGLVVLLAAFT
                     GAMCAYQLPGKAHGQGIPVPVVIWGAVVVAVAFVVRRTRFGRYVFALGGNPEAARLVG
                     IPVKRVTLQMFVLLSITITIAAIVAVSRLNAGTNSLGTGMELYVIAAAVIGGVSLAGG
                     SGSVLGTVLGALIIQFLDSGLLLLDVGIGQRMIIIGQVLIVAVAFDVLYRRWSGERVA
                     "
     gene            complement(2625..3647)
                     /gene="xylF"
                     /locus_tag="VF453_01875"
     CDS             complement(2625..3647)
                     /gene="xylF"
                     /locus_tag="VF453_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_function: GO:0033222 - xylose binding [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7686419.1"
                     /translation="MTFKKPLLAAALLAAFAAHAEPLVVGVSWSNFQEERWKTDEAAI
                     KAELAKLGATYVSADAGGSPEKQLADVDGLLAKGAKALIVLAMDKDAIVPALAKAKQR
                     NVPVVAYDRLIEQPGVFYITFDNKEVGRMQARAVFSVKPAGNYVMIKGSPTDPNADFL
                     REGQQEVLAPAIKSGAIKIVGEEYTEGWKPDVAQKNMEQILTRNNNKVDAVVASNDGT
                     AGGVVAALSARGISGVPVSGQDGDQAALNRVALGTQTVSVWKDARQLGKEAADVAVDL
                     ASGKKVPGATTWSGGEHKVPMQSLFLKPVPITKANLDIVVKAGWITKDALCKDVRAAA
                     APKACK"
     gene            3843..>4686
                     /locus_tag="VF453_01880"
     CDS             3843..>4686
                     /locus_tag="VF453_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX7686420.1"
                     /translation="MQSPPLTGDQRFVKTINRMALLRLLRDEPGISRAALAERSGLTK
                     STVSLLAKELIDEGWLAEDDAAVTGSLGRRPTPLRLDGRNLAMIGADLGTDSIRIVGA
                     SVQGEVLESSTVPLRSRAPEAACHQLVRLVTAQSAKVTAAGKRLLGIGVGLPGAVDTQ
                     SGILEFAPNIGWRKVEVGKRLAFELESARLGDVPVYVQNEADLAAVGETEFGTRPAED
                     PLVYVSCSVGVGAGIVLDGALFTGATGAAGEIGHTTLHVDGLPCSCGRRGCAEAYVGL
                     KAVAR"
BASE COUNT          682 a         1689 c         1668 g          647 t
ORIGIN      
        1 cgagctcggg catctgcgcg cgccgcatgc cgccggcgtc gagcagcgcg tccgaccacg
       61 cccgcgcccc gacgtccagc cacagcgtgc cggcggcgtc ggacatgtcg ctgaacgcgc
      121 tgccggtcag gcgccagcgc agccagtcct tgggcagcag cacgcgggcg acgcgggcgt
      181 gcacgtcggg ctcgtggcgc tcgatccaca gcagcttggg cgccgtgaag ccgggcatcg
      241 cgaggttgcc cgcgatcgcc ggcagcgccg gcatccggcg cacgagctcg tcgcactcgg
      301 cgccgctgcg cgtgtcgttc cagaggatgg ccggccgcag caccatgccg gcggcgtcga
      361 gcagcacggc gccgtgcatc tggcccgcga ggccgatcgc ctgcacggcc gcgaactcgg
      421 cgggatgcgc ggcgcgcagc gtggccgcgg cggcgacgag cgcgtcccac cagtcctgcg
      481 gatgctgctc cgaccagtgc ggccgcgggc gggagaccgc cagcggcgcg cccgccgtcg
      541 cgagcaggcg gccgtcctcg cccagcagcg ccagcttcag ctcggacgtg ccgaggtcga
      601 taccaagaac cgtattcacg cggatacccc acgatgtact tccgcgacat gccgcacggg
      661 cttgccgccg atgatcatgc ccagcacgtc gtcctcgctc aggctggcgg tgcggtaggt
      721 gccgaccatg cggccgttct tcatgaccgc gaggcgatcg gacaggccga acacgtcggg
      781 catgtcgtgc gtgatcagga agatgcccac gccctgcgcc ttcagccgct tcacgagctc
      841 gtgcaccatg cgcgtctctt ccgggccgag ggcggcacag ggttcgtcca tcaccagcac
      901 gcgggcgttg aagtgcaggg cgcgcgagat cgcgaccacc tggcgctgcc cgcccgagag
      961 cgcccgcacc ggcacgcgga tgttgcggaa gttcgggttc agctgcgcga acaccctgcg
     1021 cgcggcggcc tccatcgcgt agtcgtccag cgtgttccag cgcgtcatga tctcgcggcc
     1081 gaggaacagg ttggccaccg cgtcgaggtt gtcggccagc gcgagcgtct ggtggatcgt
     1141 ctcgatgccc agggcctggg cgtcggcggg cttctcgatg cgcgcggcct ggccgccgac
     1201 gcggatctcg ccgcggtcga ccgcgaaggc gcccgcgagc atcttcatca gggtcgactt
     1261 gccggcgccg ttgtgcccga gcacggcgac gacctcgccc gggtcgagcg cgaggctgac
     1321 gtcctcgacg gcgtgcaccc cgccgaagga cttgcccacg cccgcgaatt cgatgagggc
     1381 ggaggtcgtc atgcgacacg ctccccgctc cagcgtcgat agagcacgtc gaacgcgacg
     1441 gcgacgatga gcacctggcc gatgatgatc atgcgctggc cgatgcccac gtccagcagc
     1501 agcaggccgc tgtcgaggaa ctggatgatc agcgcgccaa ggacggtccc cagcaccgag
     1561 ccgctgccgc ccgcgaggct cacgccgccg atgaccgcgg cggcgatcac gtagagctcc
     1621 atgccggtgc ccagcgaatt ggtgcccgcg ttcagccgtg agaccgcgac gatggcggcg
     1681 atcgtgatcg tgatcgacag cagcacgaac atctgcagcg tcacgcgctt gacggggatg
     1741 ccgaccagcc gcgcggcctc cgggttgccg cccagcgcga acacgtagcg cccgaagcgc
     1801 gtgcgtcgca cgacgaaggc gacggcgacg accaccgcac cccagatgac gaccggcacc
     1861 gggatgccct ggccgtgcgc cttgccgggc agctggtagg cgcacatcgc gccggtgaac
     1921 gcggccagca gcacgacgag gccgagcgtg aagccgaggt cggcccacag cggccgcaac
     1981 gggaagccgt ggcgcgcgcg ggcgcggcgc atgcgcgcga tgcgcacggc cagcagcgcg
     2041 gcgagcacgg ccgcgagcgc ccagctgccg acgatgccca gcgcgccgtc caggccgccg
     2101 ccgaaccaca ggaagaccgg gtcggtgacg ggctgcgtct tgccgtcggc cacgaggaag
     2161 gccgcgccgc ggaacgacat cagcccgccc agcgtgacga cgaaggacgg cacgccgagg
     2221 taggcggtga ggccgccctg gtacagcccg acggcgatcg ccgcgagcag cccgacgccg
     2281 caggccgcca tccagctcca gccgtgcgtg tactgcagcc aggcgatgag cacgccgacg
     2341 aagcccatca cggagccgac ggacaggtcg atgtgccggc ccaccacgac cagcgcgatc
     2401 gcggtggcga cgatgcccac gaccgcggtc tgctgggcga tgttgtagag gttctccggc
     2461 gtgaggaagt tgccggtgag gacgtggaag acgacggcca tcgcggccag cacggccgcc
     2521 atcaaggcca gccgcaggtc gagcgcggac agttgcagcg attccatggc gagaagaggc
     2581 gggcgcgccc ggccgcgcgg gccgggcgcg ccatcaggtg gcgtttactt gcaggccttg
     2641 ggcgccgcgg cggcccggac gtccttgcac agcgcgtcct tcgtgatcca gccggccttg
     2701 acgacgatgt ccaggttggc cttggtgatc ggcaccggct tcaggaacag cgattgcatc
     2761 ggcaccttgt gctcgcctcc gctccaggtc gtcgcgccgg gcaccttctt gccgctcgcg
     2821 aggtcgacgg cgacgtccgc cgcctccttg cccagctggc gcgcgtcctt ccacaccgag
     2881 acggtctgcg tgcccagcgc gacgcggttg agcgccgcct ggtcgccgtc ctggcccgag
     2941 accggcacgc ccgagatccc gcgcgccgac agcgccgcga ccacgccgcc ggccgtgccg
     3001 tcgttggagg ccaccaccgc gtcgaccttg ttgttgttgc gggtgaggat ctgctccatg
     3061 ttcttctgcg cgacgtcggg cttccagccc tcggtgtact cctcgccgac gatcttgatc
     3121 gcgcccgact tgatcgccgg cgcgagcacc tcctgctggc cttcgcgcag gaagtccgcg
     3181 ttcgggtcgg tgggcgagcc cttgatcatc acgtagttgc cggccggctt gacggagaac
     3241 accgcgcgcg cctgcatgcg gccgacctcc ttgttgtcga aggtgatgta gaagaccccc
     3301 ggctgctcga tgaggcggtc gtaggcgacc acggggacgt tgcgctgctt ggccttcgcg
     3361 agcgcgggga cgatggcgtc cttgtccatc gccagcacga tcagcgcctt ggcgcccttg
     3421 gcgagcaggc cgtcgacgtc ggcgagctgc ttctcgggcg agccgcccgc gtccgcgctc
     3481 acgtaggtcg cgcccagctt ggcgagctcg gccttgatgg cggcctcgtc ggtcttccag
     3541 cgttcttcct ggaagttcga ccagctcacg cccaccacga gcggctcggc atgcgccgcg
     3601 aacgccgcca gcagggcggc ggccaggaga ggcttcttga aggtcatggt ttgtctccgg
     3661 tgtcgtcttt gcttgtgcac ggcgcacccg ctcctcggga acgcgaaccg gagattactt
     3721 tgatcgacgg tcgaactaat tggaggtttc ccggatgccg ggcacgcgcg tcgcgactag
     3781 gctgggccgg gccgacaatg cgcgctcccc cacgcaagaa gatcaacgaa agacaacgcg
     3841 gcatgcaaag ccccccgctc acgggcgacc aacgcttcgt caagaccatc aaccgcatgg
     3901 ccctgctgcg cctgctgcgc gacgagccgg gcatctcgcg ggccgcgctg gccgagcgca
     3961 gcggcctgac caagtccacc gtcagcctgc tcgccaagga gctgatcgac gagggctggc
     4021 tggccgagga cgacgccgcc gtcaccggct cgctcgggcg ccggccgacg ccgctgcgcc
     4081 tggacggccg caacctcgcg atgatcggcg ccgacctcgg caccgacagc atccgcatcg
     4141 tcggcgcctc ggtgcagggc gaggtgctcg agtcgagcac ggtgccgctg cgctcgcgcg
     4201 cgcccgaggc cgcctgccac cagctcgtgc gcctcgtgac cgcgcagtcg gccaaggtga
     4261 cggcggccgg caagcggctg ctgggcatcg gcgtcgggct gcccggcgcg gtcgacacgc
     4321 agtccggcat cctcgagttc gcgcccaaca tcggctggcg caaggtggag gtcggcaagc
     4381 ggctcgcgtt cgagctcgag tccgcccggc tgggcgacgt gccggtctac gtgcagaacg
     4441 aggccgacct cgcggccgtg ggcgagaccg agttcggcac gcggcctgcc gaggatccgc
     4501 tcgtctacgt gagctgcagc gtgggcgtcg gcgcgggcat cgtgctcgac ggggcgctgt
     4561 tcacgggcgc gaccggcgcc gccggcgaga tcggccacac gacgctgcac gtcgacggcc
     4621 tgccctgctc ctgcggccgc cgcgggtgcg cggaggccta cgtcggcctc aaggccgtcg
     4681 cccggg
//
LOCUS       DASVLN010000088         2750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_166086, whole genome shotgun sequence.
ACCESSION   DASVLN010000088 DASVLN010000000
VERSION     DASVLN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166086"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VF453_01885"
     CDS             complement(<1..1630)
                     /locus_tag="VF453_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7686421.1"
                     /translation="MIHFRIEVADAAAHQFKVTLTIPDPAAEQHLRLPAWIPGSYMLR
                     EFARHLSRVEARQGREPRVVTQHGKDHWSVAASGRAALVVTYFAYAFDASVRTAWLGP
                     DRGFFNATSLCFAVEGRTGEPQRIELGRLPAGWDVATAMARVDAGGKAARQAPNLFES
                     ADYDELADHPFELGPFWRGTFTACGVPHEFVVAGAWPQFDGARLLADTRRICEEEIRF
                     WHGNGKPPFARYVFMLYATEDGYGGLEHRASTALIAARKDLPRLGQPGMSDGYVTLLG
                     LISHEYFHTWNVKRLKPAEFLPYDFTRENYTELLWFFEGFTSYYDDLFLVRSGLVDAG
                     RYVKLLGRAMNGVRQSPGQHVQSVAEASFDAWVKYYRQDENTPNSTVSYYTKGSLVAL
                     MLDLALRAGRKATLDDVMRTLWREAPGGAVTEELILATVARLGGKAVAAELRRWVHER
                     AELDIVPALAKTGVELDQEPLTLAAELGLRLAEGPVTGIQVKSVLAGGAAAVAGVSSG
                     DEILAVDGWRVRRLDDAQGWLRAGAGFELLVVRQQ"
     gene            complement(1719..2498)
                     /locus_tag="VF453_01890"
     CDS             complement(1719..2498)
                     /locus_tag="VF453_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEX7686422.1"
                     /translation="MTPIRGALRLLAVLALGGACLSAWADEGAIRKAFAERYPKMKPI
                     EEVTKTPIAGLYEVRVGADLYYADEKGDYIMLVGRDTLDGHIVDTRAKVDLTEERMNR
                     ILAADIPKLPYKDAIVRKYGNGSRHMILFEDPNCHYCRDVERNLPQLKDVTIYTFVIP
                     ILGPDSVMKARDIWCSKDNASTWRNWMLDGVMPTRGMGQCDVAVLDRNRELADRFRVN
                     GTPAIIFDDGSRFAGAVDLAHLSKRLDDVAAGTKRHLEPQG"
     gene            complement(2523..>2750)
                     /locus_tag="VF453_01895"
     CDS             complement(2523..>2750)
                     /locus_tag="VF453_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HEX7686423.1"
                     /translation="AERESWRPLGDEALLRRYARARLWPTRAMAGAVDGLWQLFSAPA
                     PGLRELRNQGMTLVNHLSPLKRWLVARALAS"
BASE COUNT          376 a          942 c         1033 g          399 t
ORIGIN      
        1 gctgctggcg cacgacgagc agctcgaagc ccgcgccggc gcgcagccag ccctgggcgt
       61 cgtcgagccg gcgcacgcgc cagccgtcga ccgccaggat ctcgtcgccc gaggacacgc
      121 ctgccaccgc agcagcgccg ccggccagca ccgacttcac ctggatgccg gtcaccggcc
      181 cctcggccag gcgcaggccc agctccgcgg ccagcgtcag cggctcctgg tcgagttcga
      241 cgccggtctt cgcgagcgcg ggcacgatgt cgagctccgc gcgttcgtgc acccagcgcc
      301 gcagctccgc ggcgacggcc ttgccgccca ggcgggcgac ggtcgcgagg atgagctcct
      361 cggtcacggc gccgcccggc gcctcgcgcc acagcgtgcg catcacgtcg tcgagcgtcg
      421 ccttgcgccc ggcccggagc gcgaggtcca gcatcagcgc gaccagcgag cccttcgtgt
      481 agtagctgac cgtcgagttc ggcgtgttct cgtcctggcg gtagtacttg acccaggcgt
      541 cgaagctcgc ctcggccacg ctctggacgt gctggcccgg cgactggcgc acgccgttca
      601 tcgcgcggcc gagcagcttg acgtagcggc ccgcgtcgac gaggccgctg cgcacgagga
      661 acaggtcgtc gtagtaggac gtgaagcctt cgaagaacca cagcagctcg gtgtagttct
      721 cgcgcgtgaa gtcgtagggc aggaactcgg ccggcttcag gcgcttgacg ttccaggtat
      781 ggaagtattc gtggctgatc aggcccagca acgtcacgta gccgtcggac atccccggct
      841 ggccgaggcg gggcaggtcc ttgcgcgcgg cgatcagcgc ggtgctcgcg cggtgctcga
      901 ggccgccgta gccgtcctcg gtcgcgtaga gcatgaacac gtagcgcgcg aacggcggct
      961 tgccgttgcc gtgccagaag cggatctcct cctcgcagat gcggcgcgtg tcggcgagca
     1021 ggcgcgcgcc gtcgaactgc ggccaggcgc cggcgacgac gaactcgtgc gggacgccgc
     1081 aggccgtgaa cgtgccgcgc cagaacggcc cgagctcgaa cgggtggtcg gcgagctcgt
     1141 cgtagtcggc ggactcgaac aggttcgggg cctgcctggc cgccttgccg cccgcgtcga
     1201 cacgcgccat cgcggtggcg acgtcccacc cggcgggcag gcgcccgagc tcgatgcgct
     1261 ggggctcgcc ggtgcggccc tcgacggcga agcacaggct ggtcgcgttg aagaagccgc
     1321 ggtcgggacc gagccacgcg gtgcgcaccg aggcgtcgaa cgcgtaggcg aagtaggtca
     1381 cgaccagcgc cgcgcggccc gaggcggcga cgctccagtg atccttgccg tgctgcgtga
     1441 cgacgcgcgg ctcgcggccc tggcgcgcct cgacccggct caggtggcgc gcgaactcgc
     1501 gcagcatgta gctgccgggg atccaggccg gcagccgcag gtgctgctcg gcggccgggt
     1561 cggggatggt cagcgtgacc ttgaactggt gggccgccgc gtcggcgacc tcgatccgga
     1621 agtgaatcat gcggcggagg ccggctggcg ggcgggggac atcgtcctat tgtcgcccgg
     1681 acgccgggcc ggcgccggga cgcccccggg cgccgcggtc agccctgcgg ctcgaggtgg
     1741 cgcttcgtgc ctgcggcgac gtcgtcgagc cgcttggaca ggtgcgcgag gtcgaccgcg
     1801 ccggcgaagc gcgagccgtc gtcgaagatg atcgcgggcg tgccgttgac gcggaagcgg
     1861 tccgcgagct cgcggttgcg atcgagcacg gcgacgtcgc actggcccat gccgcgcgtg
     1921 ggcatcacgc cgtcgagcat ccagttgcgc caggtgctcg cgttgtcctt cgagcaccag
     1981 atgtcgcgcg ccttcatcac cgagtcgggg ccgaggatcg ggatcacgaa ggtgtagatc
     2041 gtcacgtctt tgagctgcgg gaggttgcgc tccacgtcgc ggcagtagtg gcagttcgga
     2101 tcctcgaaca ggatcatgtg gcggctgccg ttgccgtact tgcgcacgat ggcgtccttg
     2161 tacggcagct tggggatgtc ggccgcgagg atgcggttca tgcgctcctc ggtcaggtcg
     2221 accttggcgc gcgtgtcgac gatgtggccg tccagcgtgt cgcggccgac cagcatgatg
     2281 tagtcgccct tctcgtcggc gtagtagagg tcggcgccga cgcgcacctc gtacaggccg
     2341 gcgatcggcg tcttcgtgac ctcctcgatc ggcttcatct tcggatagcg ctcggcgaag
     2401 gccttgcgga tggcgccctc gtcggcccag gccgagaggc aggcgccgcc gagggcgagc
     2461 acggcgagca ggcgcagggc gccacggatc ggggtcatgg ggacgtcctt gtgggaggcg
     2521 gctcaggatg cgagcgcgcg cgcgaccagc cagcgcttga gcgggctgag gtgattgacc
     2581 agagtcatgc cctggttgcg aagttcccgc agcccggggg ccggcgcgga gaaaagctgc
     2641 cacagcccgt cgaccgcgcc cgccatcgcg cgcgtgggcc acagccgggc gcgggcgtag
     2701 cggcgaagca gggcctcgtc gccgagcggc cgccaggact cgcgctcggc
//
LOCUS       DASVLN010000089         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_434526, whole genome shotgun sequence.
ACCESSION   DASVLN010000089 DASVLN010000000
VERSION     DASVLN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434526"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VF453_01900"
     CDS             complement(<1..833)
                     /locus_tag="VF453_01900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX7686424.1"
                     /translation="MRDAQVIVLATPVFFLLIAIELAVAWLRGVAAYAWPDTLSSIGL
                     GMLSQLVGLFTKLFTLGLYTWAYEHARLATLDASSPAVWLAALLAYDFCYYWLHRAGH
                     RVALLWAAHVVHHQSEEYNLSTALRQTGSGWLAGWLFYLPLAVAGVPPLVFAVVALVD
                     LLYQFWVHTRLVGSLGWFDRVFCAPSNHRVHHAVNDVYLDRNFGGILVVWDRWFGTFQ
                     PELPAEPCVYGTRSPLRSWNPLWANVEVYAVLAHDSRHARSLADKLRLWFKPPGWRPA
                     DV"
     gene            complement(912..1550)
                     /locus_tag="VF453_01905"
     CDS             complement(912..1550)
                     /locus_tag="VF453_01905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7686425.1"
                     /translation="MGDATRLIAVRHGETAWNVEARLQGQLDIPLNPRGADQARRAAR
                     VLSDDAIDVVVSSDLARAHATAQAIASFNRCPLLLEPGLRERSFGSFQGLTHQEVADR
                     WPEQSARWKSRDPDFAPGDGESLRTFFERCVEATLRIAEAHAGRTVVLVAHGGVMDCL
                     YRAASRIPLDAQRSWSLDNAAINRLLHTDSGLTLVGWNDVGHLADPALDEIA"
     gene            complement(1555..>1940)
                     /locus_tag="VF453_01910"
     CDS             complement(1555..>1940)
                     /locus_tag="VF453_01910"
                     /inference="COORDINATES: protein motif:HMM:NF020469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEX7686426.1"
                     /translation="ATADRRQSRPAAALAACEVRPIMMYKFKSRAAADLLMTEPVGNR
                     VLSLMGKEHTRQGIIDVDQLGPAIAALEAAVAAECPNPPCHDEDDDRPHASSSDHVSL
                     RQRVWPMVQMLKAAQAEEQPVVWGV"
BASE COUNT          283 a          654 c          748 g          255 t
ORIGIN      
        1 acgtcggccg gccgccagcc cgggggcttg aaccagaggc ggagcttgtc cgcgagcgag
       61 cgtgcgtgcc ggctgtcgtg cgccagcacg gcgtagacct cgacgttggc ccacagcggg
      121 ttccagctgc gcagcgggct gcgcgtgccg tagacgcagg gctcggcggg cagctccggc
      181 tggaaggtgc cgaaccatcg atcccagacc acgaggatgc cgccgaagtt gcggtcgagg
      241 tagacgtcgt tgaccgcgtg gtgcacgcga tggttggagg gcgcgcagaa cacccggtcg
      301 aaccagccga gcgagccgac gagccgcgtg tgcacccaga actggtacag caggtcgacg
      361 agcgcgacca ccgcgaacac gagcggcggc acgccggcga cggccagggg caggtagaac
      421 agccagcccg cgagccagcc ggaaccggtc tgccgcagcg cggtcgagag gttgtattcc
      481 tcgctctggt ggtgcaccac gtgcgccgcc cacaggagcg cgacgcggtg gccggcgcgg
      541 tgcagccagt agtagcagaa gtcgtaggcg agcagcgccg cgagccagac ggcgggcgag
      601 gacgcgtcca gcgtcgcgag gcgcgcgtgc tcgtacgccc acgtgtacag cccgagcgtg
      661 aagagcttcg tgaagaggcc gaccagctgg ctgagcatcc ccaggccgat gctgctgagg
      721 gtgtcgggcc atgcgtacgc cgcgacgccc ctcagccacg cgacggcgag ctcgatcgcg
      781 atcagcagga agaacacggg cgtggccagc acgatgacct gggcgtctcg catcggatgt
      841 ctcgcgtcgt cgcggcgccc gctcgggggc gggcatcgcc gggtggggcc ggctcgggcg
      901 cggacgggac ctcaggcgat ctcgtcgagc gcgggatcgg ccaggtgccc cacgtcgttc
      961 cagcccacca gcgtgaggcc ggagtccgtg tgcagcagcc ggttgatggc cgcattgtcg
     1021 agcgaccagc tgcgctgcgc atccagggga atccgcgatg cggcgcggta caggcagtcc
     1081 atcacgccgc cgtgcgcgac gagcacgacg gtgcggccgg cgtgcgcctc ggcgatgcgc
     1141 agcgtggcct cgacgcagcg ctcgaagaac gtgcgcaggg actcgccgtc gcccggcgcg
     1201 aagtcgggat cgcggctctt ccagcgcgcg gactgctcgg gccagcggtc cgcgacctcc
     1261 tggtgcgtca ggccctggaa gctgccgaag ctgcgttcgc gcaagcccgg ctcgagcagc
     1321 agcgggcagc ggttgaacga cgcgatcgcc tgcgcggtgg cgtgggcgcg cgcgaggtcg
     1381 ctgctgacga cgacgtcgat cgcgtcgtcc gagagcacgc gggccgcgcg ccgggcctgg
     1441 tcggcgccgc gcgggttgag cgggatgtcg agctggccct gcagccgcgc ctcgacgttc
     1501 caggccgtct cgccgtggcg gacggcgatc agccgcgtgg cgtcgcccac cggttcagac
     1561 gccccagacg accggctgct cctcggcctg cgcggccttg agcatctgca ccatcggcca
     1621 gacgcgctgg cgcagcgaca catggtcgga cgagctcgca tgcgggcggt cgtcgtcctc
     1681 gtcgtggcag ggggggttcg ggcactcggc ggccacggcg gcctcgaggg cggcgatcgc
     1741 ggggccgagc tggtcgacgt cgatgatgcc ctggcgcgtg tgctccttgc ccatgaggga
     1801 cagcacgcgg ttgccgaccg gctcggtcat gagcaggtcc gcggcggcgc ggctcttgaa
     1861 cttgtacatc atgatgggac ggacctcgca ggcggccagg gcggcagctg gtcgcgattg
     1921 tcgccgatcc gccgtcgcca
//
LOCUS       DASVLN010000090         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_219172, whole genome shotgun sequence.
ACCESSION   DASVLN010000090 DASVLN010000000
VERSION     DASVLN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219172"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..512
                     /locus_tag="VF453_01915"
     CDS             120..512
                     /locus_tag="VF453_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686427.1"
                     /translation="MMLITRDQMTRFDAAVFDAFTDRLADFVRERCGAPHGWQPALVP
                     EDPAELRRQVRAHLARAQGYGIRDQDALAVFVSLAFSYSPRFDSLPGAHALLRDEART
                     PDERVFSLLDVVVAAERRRRIPAHLQRR"
     gene            509..1174
                     /locus_tag="VF453_01920"
     CDS             509..1174
                     /locus_tag="VF453_01920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF025874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HEX7686428.1"
                     /translation="MTDAAAPTAAAPAPDYFEQLGEDAKHVFPPAPAQGAPVECPCQA
                     LARWRAQNKASITQALAGQRQMLLDKKAALARWNDADQAAFQKSFGTTTDAAKATIAG
                     RIDRMLDLNSKMTVDNFVLADPSKAGRFAFVHRDDMTHTVNIDGEFYDSPMTGIDSKP
                     GALSHEMSHFTDIGDTFDKFDGYNADKPVYGPDASHLLAQTRPDLALQHADSFEYYLE
                     GAP"
     gene            1269..1652
                     /locus_tag="VF453_01925"
     CDS             1269..1652
                     /locus_tag="VF453_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686429.1"
                     /translation="MPANPLRVQMHVDTSLPKRVLVTVVYDNVSDQPIWIEKDGLNLF
                     VYCEGAPIMDIGPSEKRRAPTLADFEQVAPGKSTTRQADIAQQFDWRPGRHVYTLKTS
                     GSYDDPVAHRIVDGPAVKVEFSLSR"
     gene            complement(1744..>2834)
                     /locus_tag="VF453_01930"
     CDS             complement(1744..>2834)
                     /locus_tag="VF453_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686430.1"
                     /translation="ACAVAALAVVAASGAPAIAIGLGIAGLVISQFSRYPGARGLRAW
                     LVVGMAAGALVAAAGHAWAWRVSLHANSPWEMVRLCAWFLWPGWLLALWTLWRWRHHL
                     AYRHIAVPSAGAAVALIAAISMGASDRALMLAVPGVAVLAAFALPTLKRATGAAIDWF
                     SVFFFTGFAGVIWVFWLSLTTGTPAKPAERVARLLPGFESHFRPLPLAIAAVGTVAWL
                     ALVRWRTARVQHALWKSLVLPASGVALSWLLLLTLGLPAIDYARSYRPWVALVENDVP
                     PGVCVAAQLPRSALAALEIYANWKLDARLDPAHTACPYLVVDENRHALAPTPPGWTLI
                     EHLHRPSERDESTAIFRREGVPAWKPER"
BASE COUNT          467 a         1032 c          974 g          361 t
ORIGIN      
        1 cccgggcggc gcgcacgcgg gcataatcgc gccttccgaa aaggccggcc ggcccgcatt
       61 gccgatgacc gccggaaacc attccaatag cgccgcacgg gaaatgcagg cgcagctgca
      121 tgatgctgat cactcgagac cagatgaccc ggttcgacgc cgcggtcttc gacgcattca
      181 cggaccggct ggccgatttc gtgcgcgagc gctgcggcgc gccgcacggc tggcagccgg
      241 ccctcgtgcc ggaggatccg gccgaacttc ggcgccaggt gcgtgcccac ctcgcgcgtg
      301 cgcagggcta cggcatccgc gaccaggacg cgctggcggt cttcgtgtcg ctcgcgttct
      361 cgtattcgcc gcgtttcgac agcctgccgg gcgcgcacgc cctgctgcgg gacgaggcgc
      421 gaaccccgga cgagcgcgtc ttctcgttgc tcgacgtcgt ggtggccgcc gagcgccgcc
      481 gccgcattcc cgcccacctg caacgccgat gaccgacgcc gccgcaccaa ccgccgccgc
      541 accggcgccc gactacttcg agcagctcgg cgaggacgcc aagcacgtgt ttccccccgc
      601 gccggcgcag ggtgcgcccg tggagtgccc ctgccaggcc ctggcccgct ggcgagcgca
      661 gaacaaggcc tcgatcacgc aggcgctcgc ggggcagcgc cagatgctcc tggacaagaa
      721 ggccgcgctc gcgcgctgga acgacgccga ccaggcggca ttccagaagt cgttcggcac
      781 cacgacggat gccgcgaagg ccacgatcgc aggccgcatc gaccggatgc tggacctgaa
      841 ttcgaagatg acggtggaca atttcgtgct ggccgatccg tccaaggccg gccgcttcgc
      901 gttcgtgcat cgcgacgaca tgacgcacac cgtgaacatc gacggtgagt tctatgattc
      961 gccgatgacc ggcatcgatt cgaagccggg ggcgctttcg cacgagatgt cgcacttcac
     1021 ggacatcggc gacacgttcg acaagttcga tggctacaac gccgacaagc cggtctacgg
     1081 ccccgacgcg tcgcacctgc tcgcacagac gcgtcccgac ctcgccttgc agcacgcaga
     1141 cagctttgaa tactatctcg agggtgcgcc ctgaattcca ccgccgccgg ctcctgctct
     1201 gcggcgtgct cgcgccgctc gccgcgcccc ttctcgcatg cgccgtcgcg ccgacggccg
     1261 gacaaacgat gccagccaat cccttgaggg tacagatgca tgtcgacacg tcgctgccca
     1321 agcgcgtgct cgtcaccgtg gtgtacgaca acgtctcgga tcagccgatc tggatcgaga
     1381 aggacgggct gaacctgttc gtgtactgcg agggggcgcc gatcatggac atcggcccgt
     1441 ccgagaagcg gcgcgcaccg acgctcgccg acttcgagca ggtggcaccg ggcaagtcga
     1501 cgacccgtca agccgacatc gcgcagcagt tcgactggcg tcccgggcgc cacgtctaca
     1561 cgctcaagac ttcgggcagc tacgacgatc cggtcgcgca ccggatcgtc gacggcccgg
     1621 cagtgaaagt cgagttctcg ctctcgcgct gacgaccgcg atccggccgg cgctgcgtcg
     1681 cggtcgccgg acgtcccggc gccaccaagc cgccctggca tgccgccccg cgccgagcag
     1741 gtatcaccgc tccggcttcc acgccggcac gccctcgcgg cggaagatcg ccgtcgattc
     1801 gtcgcgctcg gagggccggt gcaggtgctc gatgagcgtc cagcccggcg gcgtgggcgc
     1861 gagcgcgtgg cgattctcgt cgacgacgag atacgggcag gcggtatgcg cgggatcgag
     1921 gcgggcgtcc agcttccagt tcgcatagat ttccaacgcc gccagcgcgg agcggggcag
     1981 ctgcgcggcg acgcagacgc cgggcggcac gtcgttctcg accagcgcga cccagggccg
     2041 gtagctgcgc gcgtagtcga tcgcgggcag gcccaacgtc aggagcagca gccagctgag
     2101 cgcgacgccc gaggccggca gcacgagcga cttccacagc gcgtgctgaa cccgcgccgt
     2161 acgccagcga acgagcgcca gccaggccac cgtgccgacg gccgcgatgg ccagcggcag
     2221 cgggcggaag tgcgattcga agccgggcag caggcgggcg acgcgctcgg cgggcttggc
     2281 gggcgtgccg gtcgtcagcg agagccagaa cacccagatc acgccggcga agccggtgaa
     2341 gaagaagacc gagaaccagt cgatcgccgc gcctgtcgcg cgcttgagcg tcggcagcgc
     2401 gaacgcggcg agcaccgcga cgccgggcac ggccagcatc agcgcgcggt cggatgcgcc
     2461 catcgagatc gccgcgatga gcgcgacggc cgcgccggca ctcgggacgg cgatgtggcg
     2521 gtaggcgagg tggtggcgcc agcgccagag cgtccacagc gcgagcagcc agcccggcca
     2581 caggaaccac gcgcacaggc gcaccatctc ccacggcgag ttcgcgtgca ggctcacgcg
     2641 ccacgcccag gcatgtccgg cggccgcgac gagcgcgcct gctgccatgc cgaccaccag
     2701 ccacgctcgc agtccgcgcg cgcccgggta gcgcgagaac tgcgagatca cgagccccgc
     2761 gatgcccagg ccgatggcga tggccggcgc gccgctggcc gcgaccaccg cgagcgccgc
     2821 gaccgcgcag gcgg
//
LOCUS       DASVLN010000091         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_57876, whole genome shotgun sequence.
ACCESSION   DASVLN010000091 DASVLN010000000
VERSION     DASVLN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57876"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            510..1757
                     /gene="glyA"
                     /locus_tag="VF453_01935"
     CDS             510..1757
                     /gene="glyA"
                     /locus_tag="VF453_01935"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7686431.1"
                     /translation="MFDRQQSTIANIDPEVWAAIQAENTRQEEHIELIASENYTSPAV
                     MAAQGSQLTNKYAEGYPGKRYYGGCENVDVIEQLAIDRVKKLFGAEAANVQPNSGSQA
                     NQAVFLGLLQPGDTIMGMSLAEGGHLTHGMALNMSGKWFKVVSYGLNAQEDIDYDAME
                     RTAREHKPKLIIAGASAFALRIDFERFARIAKEIGAYFMVDMAHYAGLIAAGVYPNPV
                     PHADVVTSTTHKSLRGPRGGIILMKEAFAKPINSAIFPGLQGGPLEHVIAGKAIAFLE
                     ALQPAFKTYQEQVVKNADVLAKTLIERGLRIVSGRTESHVMLVDLRAKNITGKEAERV
                     LGLAHMTCNKNAIPNDPEKPMVTSGIRLGTPAMTTRGFKEAEARATANLIADVLEAPQ
                     DEGRLAEVRAKVAALTREFPVYR"
BASE COUNT          312 a          691 c          602 g          218 t
ORIGIN      
        1 ttcttcaacg acgccgcggc cttccgcggc gtcgtcgtct ccggggatcg cacggccgcg
       61 acacgtccgc cgcctgccgg cccggtcggg ccgccggaac gatcccgcga ctcgcctgcg
      121 agaccgggcc tgcggccggc gccggcacga agcgccaaat ccttcgtaaa ccgacaattc
      181 cgcgcgcgga cgcccgcagg gcggatccgg cgcgctaaac tggcgactcg cgcgactggc
      241 gacaggacgc gccccgggag accggccccg ctgtggcagc agccggcggc cggctcgcca
      301 cgggggcgtt gcgaggtggg ccaccaccgg gaagcgcgaa ggacggccgc cggctccggc
      361 gtcccggcct gtcagccgtt cgcctgggca gcgcgaccgc cgcgcaccgc cgcttcgccg
      421 atccgccgcg catccccgcg cggcgcccgc gcaggggcga gcccgtcgac gcgcccgttc
      481 agccccatcc gacgaaggat ccattcccca tgttcgaccg ccaacaatcg acgatcgcca
      541 acatcgaccc cgaggtctgg gccgccatcc aggccgagaa cacccgccag gaagagcaca
      601 tcgagctgat cgccagcgag aactacacct cgccggccgt gatggccgcg cagggctcgc
      661 agctcaccaa caagtacgcc gagggctatc ccggcaagcg ctactacggc ggctgcgaga
      721 acgtcgacgt gatcgagcag ctcgcgatcg accgcgtgaa gaagctgttc ggcgccgagg
      781 cggccaacgt gcagccgaac tccggctcgc aggccaacca ggccgtcttc ctgggcctgc
      841 tgcaacccgg cgacaccatc atgggcatga gcctggccga aggcggccac ctgacccacg
      901 gcatggcgct gaacatgagc ggcaagtggt tcaaggtcgt cagctacggc ctgaacgcgc
      961 aggaggacat cgactacgac gcgatggagc gcaccgcgcg cgagcacaag ccgaagctga
     1021 tcatcgccgg cgcgtcggcg ttcgcgctgc gcatcgactt cgagcgcttc gccaggatcg
     1081 ccaaggagat cggtgcctac ttcatggtcg acatggcgca ctacgccgga ctgatcgcgg
     1141 cgggcgtcta cccgaacccg gtgccccacg ccgacgtggt cacctcgacc acgcacaaga
     1201 gcctgcgcgg gccgcgcggc ggcatcatcc tgatgaagga ggcgttcgcc aagccgatca
     1261 actccgcgat cttccccggc ctgcagggcg gcccgctcga acacgtcatt gccggcaagg
     1321 ccattgcctt cctcgaggcg ctgcagcccg cgttcaagac ctaccaggag caggtcgtca
     1381 agaacgccga cgtgctcgcg aagacgctga tcgagcgcgg cctgcgcatc gtctcgggcc
     1441 gcaccgagag ccacgtgatg ctggtcgacc tgcgcgccaa gaacatcacc ggcaaggaag
     1501 ccgagcgcgt gctgggcctg gcccacatga cctgcaacaa gaacgcgatc ccgaacgacc
     1561 cggagaagcc gatggtcacg agcggcatcc gcctgggcac gcccgcgatg accacgcgcg
     1621 gcttcaagga ggccgaggcc cgcgccacgg cgaacctgat cgccgacgtg ctcgaggcgc
     1681 cgcaggacga gggccgcctc gccgaggtgc gcgccaaggt cgccgcgctg acgcgcgagt
     1741 tcccggtcta ccgctgaccg cccgccgcga gccccgcgcc atggcccgtg ccgcccgttc
     1801 gctccgccgc ctcgcgcgcg cgt
//
LOCUS       DASVLN010000092         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_274109, whole genome shotgun sequence.
ACCESSION   DASVLN010000092 DASVLN010000000
VERSION     DASVLN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274109"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VF453_01940"
     CDS             <1..326
                     /locus_tag="VF453_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7686432.1"
                     /translation="LVTAPADVARLQGWAAASDRATVVAAMSDLVGDDLRPRLARIHA
                     PTLVLATWAGHPGAGAREQAQATFERQYAGLAGARIAMAPTARHFVMLDDPAWFQAEV
                     GPFLR"
     gene            400..1272
                     /locus_tag="VF453_01945"
     CDS             400..1272
                     /locus_tag="VF453_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7686433.1"
                     /translation="MPVAHLLLALAVVAIWGTNFVVIRLGLDAFAPYTFAALRYTFSF
                     LPFIFFVRRPAASWGKLAAFGVLIGVGQFGLLFWAMRSDISPGLASLVIQSQVFFTIA
                     LSAAMYRERMKTLQWPAFALAVAGLAIVAAHVSASAGVTLFGVALVIAAAVAWAAGNL
                     VARAAGRVDALGFMVWSSPFAAVPLWLITWLVDGPQRVGGSLAHAGAGAWVAVAWQSI
                     GNTLFGYGVWNWLLARHPASTVTPTALLVPVFGMTASALALHEVLPGWKLFAAALVMA
                     GLAVNMLATRPRPA"
     gene            complement(1321..>1573)
                     /locus_tag="VF453_01950"
     CDS             complement(1321..>1573)
                     /locus_tag="VF453_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7686434.1"
                     /translation="QAVRERGEHDAAVRGLYAALDQAARLGGSHVRRDLLAQTLMHCA
                     ARGSGTIHVGRALLNERCLAKPVTPLTRFWAGKLQVAIA"
BASE COUNT          160 a          580 c          578 g          255 t
ORIGIN      
        1 cgctcgtgac ggcgccggcg gacgtggcac ggctgcaggg ctgggcggcg gcctccgatc
       61 gcgccaccgt ggtcgccgcg atgagcgacc tggtcggcga cgacctgcgc ccgcggctcg
      121 cccgcatcca cgcgccgacg ctggtgctcg cgacctgggc cggccatccc ggcgccggcg
      181 cgcgcgagca ggcgcaggcg accttcgagc gccagtacgc cggcctcgcg ggcgcgcgca
      241 tcgcgatggc gcccaccgcg cgccatttcg tgatgctgga cgacccggcg tggttccagg
      301 ccgaggtcgg gcccttcctg cgctgagcgc cgcgacgaac cgcagctgcg ggcaaccgga
      361 cggcggcgcg gcctttggca cgccgcctac actggctcga tgcccgtcgc ccatctcctg
      421 ctcgccctgg ccgtggtcgc catctggggc acgaacttcg tcgtcatccg cctcggcctg
      481 gacgcgttcg cgccctacac cttcgccgcg ctgcgctaca cgttctcgtt cctgcccttc
      541 atcttcttcg tccgccgccc cgcggcctcg tggggaaagc tcgcggcctt cggcgtgctg
      601 atcggggtcg ggcagttcgg gctgctgttc tgggcgatgc gctcggacat ctcgcccggc
      661 ctggcctcgc tcgtgatcca gtcgcaggtg ttcttcacga tcgcgctgtc ggcggccatg
      721 taccgcgagc gcatgaagac gctgcagtgg ccggcgttcg cgctggcggt ggcggggctc
      781 gcgatcgtcg ccgcgcacgt gtccgcgagc gccggggtga cgcttttcgg cgtggcgctt
      841 gtcatcgcgg cggcggtcgc ctgggccgcg ggcaacctcg tcgcacgtgc ggcggggcgg
      901 gtcgacgcgc tgggcttcat ggtctggtcg agcccgttcg ccgcggtgcc gctgtggctg
      961 atcacctggc tcgtggacgg tccgcagcgc gtcggcggct cgctcgcgca tgcgggtgcc
     1021 ggcgcgtggg tcgcggtcgc atggcagtcg atcggcaaca cgctgttcgg ctacggcgtg
     1081 tggaactggc tgctggcgcg ccaccccgcg tccacggtca cgcccacggc gctgctggtg
     1141 ccggtcttcg gcatgacggc ctcggcgctc gcgctgcacg aagtcctgcc gggctggaag
     1201 ctgttcgcgg cggcgctcgt gatggccggg ctcgccgtca acatgctcgc gacgcgcccg
     1261 cgtccggctt gagcgatacg ccaggcccta tcgacctgga tacccggtac cgtacgcagc
     1321 tcaggcgatc gcgacctgca gcttgccggc ccagaaacgg gtcagcggcg tcaccggctt
     1381 ggcgaggcag cgttcgttga gcagagcgcg gccgacgtgg atcgtgccgc tgccgcgcgc
     1441 cgcgcagtgc atcagcgtct gggcgagcag gtcgcgccgc acgtggctgc cgcccaggcg
     1501 agcggcctgg tcgagcgccg cgtagagccc gcgcaccgcc gcatcgtgct cgccgcgctc
     1561 gcgcacggcc tgc
//
LOCUS       DASVLN010000093         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_219343, whole genome shotgun sequence.
ACCESSION   DASVLN010000093 DASVLN010000000
VERSION     DASVLN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219343"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VF453_01955"
     CDS             complement(<1..560)
                     /locus_tag="VF453_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7686435.1"
                     /translation="MTTVLILGLGASGLAMARWCAGRGVGVRVWDSRANPPQAAALAA
                     EVPDSAFFSGDLPAAAIDGVSRVYKSPGLAPSDPRLAALMEAARAADVPVRGELDLFV
                     EALAQLKAERGYAPRVLAITGTNGKTTTTSLAGELVERAGRRVAVAGNIGPTMLDTLA
                     AALAEQAEADAIAAEEAAAQAAADAA"
     gene            complement(676..1857)
                     /gene="mraY"
                     /locus_tag="VF453_01960"
     CDS             complement(676..1857)
                     /gene="mraY"
                     /locus_tag="VF453_01960"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX7686436.1"
                     /translation="MLLSLASWLQGLDPEQFGFLRVFQYHTFRAVMAAMTALLIGLMF
                     GPWVIRRLTELKIGQPIREYGVQAHLVKRGTPTMGGVLVLIGIAVATLLWSDWSNRFV
                     WIVMIVTFGFGAIGWADDWRKVVKKNPEGMPSKEKYLWQSLIGLVAALYLAFSVSETS
                     NLRVLELFVRWVQSGFSNDLPPKADLMLPFFKTVSYPLGVFGFIALTYFVIVGASNAV
                     NLTDGLDGLAIMPVVLVAGALGLFAYIVGNAVYSKYLLFPYIPGAGELLIFCSAMAGA
                     GLAFLWFNAHPAQVFMGDVGALALGGALGTVAVITRQEIVLGIMGGVFVLEALSVMAQ
                     VSWFKYTKKKYGEGRRILKMAPLHHHFEKSGWTETQVVIRAWIITMLLCLVGLASLKL
                     R"
     gene            complement(1847..>2062)
                     /locus_tag="VF453_01965"
     CDS             complement(1847..>2062)
                     /locus_tag="VF453_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HEX7686437.1"
                     /translation="PLCAHAAQACGTQARHYADTAALLAALADPAAAPAFESALVKGS
                     RFMGMERVVALLAERDAAEGQGGAHAA"
BASE COUNT          325 a          750 c          720 g          267 t
ORIGIN      
        1 tgggcggcgt cggcggcggc ctgggcggcg gcttcctcgg cggcgatcgc atcggcctcg
       61 gcctgctcgg cgagcgccgc cgccagcgtg tccagcatcg tcgggccgat gttgccggcc
      121 accgcgacgc gccggcctgc gcgctcgacg agctcgccgg ccagcgaggt cgtggtcgtc
      181 ttgccgttcg tgccggtgat cgcgagcacg cggggcgcgt agccgcgctc ggccttcagc
      241 tgcgcgagcg cctcgacgaa caggtcgagc tcgccgcgca ccggcacgtc ggcggcgcgc
      301 gcggcctcca tcagggcggc gaggcgcgga tcggacggcg cgaggccggg gctcttgtag
      361 acgcgcgaga cgccgtcgat cgcggcggcc ggcaggtcgc cggagaagaa cgcgctgtcc
      421 ggcacctcgg ccgcaagcgc cgcggcctgc ggcgggttgg cacgcgagtc ccagacgcgc
      481 acgcccacgc cccgcccggc gcaccagcgc gccatggcca ggcccgaggc gcccaacccc
      541 aggatcagca ccgtggtcat tggagccccc cgtccgccgg gtacggcgac cggccccccg
      601 cggggtctgc gggccggctt ggggcggccc ggcgctcggc ccgcttggcg gcgatggaga
      661 agataggggt cactgtcagc gcagcttcag gctcgccagc cccacgaggc acagcagcat
      721 ggtgatgatc caggcgcgga tgacgacctg cgtctccgtc cagcccgact tctcgaagtg
      781 gtggtgcagc ggcgccatct tcaggatgcg ccggccctcg ccgtacttct tcttcgtgta
      841 cttgaaccag ctgacctgtg ccatcaccga cagcgcctcc agcacgaaga cgccgcccat
      901 gatcccgagc acgatctcct ggcgcgtgat gaccgccacg gtgccgagcg ccccgcccag
      961 ggcgagcgcg ccgacgtcgc ccatgaacac ctgggccgga tgggcgttga accagaggaa
     1021 ggccaggccc gcccccgcca tcgcggaaca gaagatcagc aactcccccg cgccggggat
     1081 gtacgggaac agcaggtact tcgagtacac cgcgttgccg acgatgtagg cgaacaggcc
     1141 cagcgcgccg gcgaccagca cgaccggcat gatcgccagg ccgtccaggc cgtcggtgag
     1201 gttgaccgcg ttgctcgcgc cgacgatcac gaagtaggtc agcgcgatga acccgaatac
     1261 acccaggggg tagctcaccg tcttgaagaa cggcagcatg aggtcggcct tgggcggcag
     1321 gtcgttggag aagccgctct gcacccagcg cacgaacagc tcgagcacgc gcaggttgga
     1381 cgtctccgac acgctgaagg ccaggtacag cgccgcgacc aggccgatca gcgactgcca
     1441 caggtacttc tccttcgacg gcatgccctc cgggttcttc ttcacgacct tgcgccagtc
     1501 gtcggcccag ccgatcgcgc cgaagccgaa ggtcacgatc atcacgatcc agacgaagcg
     1561 gttgctccag tcgctccaca gcagcgtggc gaccgcgatg ccgatcagca ccagcacgcc
     1621 tcccatcgtc ggcgtgccgc gcttgaccag gtgggcctgc acgccgtatt cgcggatcgg
     1681 ctggccgatc ttcagctcgg tcaggcggcg aatgacccac ggcccgaaca tcaggccgat
     1741 cagcagcgcc gtcatcgcgg ccatgacggc gcggaacgtg tggtactgga acacgcgcag
     1801 gaagccgaac tgctcgggat cgaggccctg cagccaggag gcgaggctaa gcagcatgcg
     1861 cgcctccctg gccctcggcc gcgtcgcgct cggcgagcag cgcgacgacc cgctccatgc
     1921 ccatgaaccg cgaacccttg accagcgccg actcgaaggc cggggcggcc gcgggatcgg
     1981 cgagcgcggc cagcagggcc gccgtgtccg cgtagtgccg cgcctgcgtg ccgcaggcct
     2041 gcgcggcgtg cgcgcacagg gg
//
LOCUS       DASVLN010000094         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_166306, whole genome shotgun sequence.
ACCESSION   DASVLN010000094 DASVLN010000000
VERSION     DASVLN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166306"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VF453_01970"
     CDS             <1..597
                     /locus_tag="VF453_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX7686438.1"
                     /translation="APRGPRAMTATTERVVALGTSTGGTQALEEVLTTLPRVCPGIVI
                     VQHMPEKFTAAFAARLDTLCRIEVREAANNDRVVPGRALIAPGGRHMLLKRSGAQYFV
                     EVVDGPVVNRHRPSVDVLFRSVAKSAGPNALGVIMTGMGDDGAAGLAEMRGAGARTLA
                     QDEDSCVVFGMPKEAIRRGGVERTVPLGAIASEILKQQ"
     gene            complement(613..>1641)
                     /locus_tag="VF453_01975"
     CDS             complement(613..>1641)
                     /locus_tag="VF453_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7686439.1"
                     /translation="PERLRAGTTPAPLVFTDLQVGGARAAAGPQARTLAIPPEAEGSF
                     AAEFSLLDFTAPERARYAYWLEGHDHGWREVDTAHRLASYTNLAPGRYRLHVKAADRD
                     GNWTGQPVSLRIDVLPAWYQSWWFLGVKLLAAGLAVTAIVAVRLRLVRRRNAQLERLV
                     GERTQELQRLQAQLEELAYSDALTGLPNRRMFGDWCRNFIAAAQRRRGGFALLLIDLD
                     HFKQVNDTRGHDAGDALLVETARRLRGVARRSDVVARLGGDEFAMLLADAADRALAEA
                     TCRRILEAFAEPAELPGGAAIRVTPSIGVALFPVDGTTQDALYKSADLALYEAKAGGR
                     NTWRFARP"
BASE COUNT          212 a          595 c          633 g          201 t
ORIGIN      
        1 gcgccgcgcg gcccgcgcgc gatgaccgcc acgaccgagc gcgtcgtcgc gctgggaacg
       61 tccaccggcg ggacgcaggc gctcgaggaa gtgctgacca cgctgccgcg ggtctgcccc
      121 ggcatcgtga tcgtgcagca catgccggag aagttcaccg ccgcgttcgc cgcgcgcctc
      181 gataccttgt gccgtatcga ggtgcgcgag gccgccaaca acgaccgcgt ggtcccgggc
      241 cgcgcgctga tcgcgccggg cgggcgccac atgctgctca agcgcagcgg cgcgcagtac
      301 ttcgtcgagg tcgtcgacgg ccccgtcgtc aaccgccacc ggccgtcggt agacgtgctg
      361 ttccgctcgg tggccaagag cgccggcccg aacgcgctgg gcgtgatcat gaccggcatg
      421 ggcgacgacg gcgccgccgg cctggccgag atgcgcggcg ccggcgcgcg cacgctcgcg
      481 caggacgagg acagctgcgt ggtcttcggc atgcccaagg aggcgatccg gcgcggcggc
      541 gtcgagcgca cggtgccgct gggcgcgatc gcgtcggaga tcctgaagca gcagtgaccg
      601 gacgccgcgc cctcagggcc gcgcgaagcg ccaggtgttg cgcccgccgg ccttggcctc
      661 gtagagcgcg agatcggcgg acttgtacag cgcatcctgc gtcgtgccgt cgaccgggaa
      721 gagcgcgacg ccgatgctcg gggtcacgcg tatcgcagca ccgccgggca gctcggcagg
      781 ctcggcgaag gcctcgagga tgcgccggca ggtcgcctcg gcgagcgcgc ggtcggcggc
      841 gtcggccagc agcatcgcga actcgtcgcc gcccagccgc gcgacgacgt ccgagcgccg
      901 cgccacgccg cgcaggcggc gcgcggtctc gaccagcagc gcgtcgccgg cgtcgtggcc
      961 gcgggtgtcg ttgacctgct tgaagtggtc gaggtcgatg agcagcagcg cgaagccgcc
     1021 gcgccggcgc tgcgccgcgg cgatgaagtt gcggcaccag tctccgaaca tgcggcggtt
     1081 gggcagcccg gtgagcgcgt cgctgtaggc gagctcctcg agctgcgcct gcagccgctg
     1141 cagctcctgc gtgcgctcgc ccaccaggcg ctccagctgc gcattgcgcc ggcgcacgag
     1201 gcgcagccgc accgcgacga tcgccgtcac cgccagcccc gcggcgagga gcttcacgcc
     1261 gaggaaccac cagctctggt accacgccgg cagcacgtcg atgcgcagcg agaccggctg
     1321 gccggtccag ttgccgtcgc ggtcggcggc cttgacgtgc agccggtagc gcccgggcgc
     1381 gaggttggtg tagctcgcga ggcggtgggc cgtgtcgacc tcgcgccagc cgtggtcgtg
     1441 gccttcgagc cagtaggcgt agcgcgcgcg ctcgggggcg gtgaagtcca gcagcgagaa
     1501 ctccgccgcg aagctgccct ccgcctcggg cgggatcgcc agcgtgcgcg cctgggggcc
     1561 cgcggccgcg cgcgcgccgc ccacctgcag gtcggtgaag acgaggggcg cgggcgtcgt
     1621 gccggcgcgc aggcgctcgg g
//
LOCUS       DASVLN010000095         3925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_166326, whole genome shotgun sequence.
ACCESSION   DASVLN010000095 DASVLN010000000
VERSION     DASVLN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166326"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF453_01980"
     CDS             <1..447
                     /locus_tag="VF453_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7686440.1"
                     /translation="VALRTAAPRPAKTPQQTLVLVVDDSKMVRLKTGKLLAAHGYQVA
                     QAVDGQDAVRLLETQLPDLVVTDVDMPNLDGFGLARHLRANARTAHVPIVMITSAEDR
                     HRDEATRAGVGLVLGKPYPEDELLAHVASFAFPAASGREAAAGAIA"
     gene            complement(490..963)
                     /locus_tag="VF453_01985"
     CDS             complement(490..963)
                     /locus_tag="VF453_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7686441.1"
                     /translation="MQLEGSCHCGAVTFTVTSHEPVPFMRCYCSICRKTAATGGYAIN
                     LGADHRTLKVRGEENLSVYQARLEGGERSTGQRNFCRHCGTALWLYDPTWPELVHPHA
                     GAIDTPLPRPTDVTHMMLGSKPDWVDAHVRPADETFDEYPKESLAEWHRRHKLDL"
     gene            1194..2630
                     /locus_tag="VF453_01990"
     CDS             1194..2630
                     /locus_tag="VF453_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX7686442.1"
                     /translation="MTDFAPPLPSPAARPWSATDDTAFLGHPRGLGWLAFSELWERFS
                     YYGMQTLLVLYMVHALLLPGHVEHVWGFDGFRRGLESVYGPLSVQALASAIFGLYAGT
                     VYLTPIAGGWIADRWLGRTTTITVGALLMALGHFLMAFEASFLIALACLLLGVGAFKG
                     NISSQVGALYGQDDPRRATAFQIFLLAVQIAVIVSPWLCGHLTEDGKYWDRGFGAAGV
                     GMVIGLLTYLGGRRWMPPEERIAASAAAAVEPRPPLSAADRRKVGLLVLLIPVLALTV
                     VGNQQIFNSYLIWAEKNLDLHLFGQEIAVTSLISLDSIISTATLLASLLFWHVWRKVL
                     REPDELMKITMGVAVAACAPLLLAGASAAVQATGHRAGIGWVVASQIVNDFGFANILP
                     VGLALYSRAAPRGMTGTMVGVYYTHLFFANMGVGWLGGQYEKMSASAFWTMHAAIVGV
                     SAVLLLLVRLTVGRGLTPAYDAPAEARA"
     gene            complement(2641..3540)
                     /locus_tag="VF453_01995"
     CDS             complement(2641..3540)
                     /locus_tag="VF453_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7686443.1"
                     /translation="MLRQPAAAAPPRTAAWVPLAPLFAVLVWTGNVLVTKRASAVIEP
                     AAITFFRWAIAFAVLTPFLARAAWRQRAVVRAHWPRIAFLGALGMSTYQGLAYEAART
                     ASAVDMGVMTATMPLLSALVARAFAAEAPSRAQAGGAIVSLLGIAWLVTRGHPAALAA
                     GGVHVGEGLMLVAVSSNALYGVLLRRWAMPLTSWQQVYVQIGFGTLVVLPFWIAGGMQ
                     PVTAASAPLVAYAALPASIAAPFFWVTGVKHHGAPRASLFLNLLPLLVAVAAWALLGE
                     PLHAFHAVGGLLVLGGVAWGLRG"
     gene            3695..>3925
                     /locus_tag="VF453_02000"
     CDS             3695..>3925
                     /locus_tag="VF453_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7686444.1"
                     /translation="MSRIDLNQLADLDALLREGSVARAAERLHLSAPAMSRRLAHLRR
                     TFGDPLFVLAGRQLVPTERALALQPRIAALLDE"
BASE COUNT          523 a         1443 c         1409 g          550 t
ORIGIN      
        1 gtcgcgctcc gcaccgccgc gccgcgcccc gcaaagacgc cgcagcagac cctggtgctg
       61 gtcgtcgacg actcgaagat ggtgcgcctg aagaccggca agctgctcgc ggcgcacggc
      121 taccaggtcg cgcaggcggt cgacggccag gacgccgtgc ggctgctcga gacccagctg
      181 cccgacctcg tcgtcaccga cgtcgacatg cccaacctcg acggcttcgg cctggcgcgc
      241 cacctgcgcg cgaacgcccg cacagcgcac gtgccgatcg tgatgatcac ctccgccgag
      301 gaccgccacc gcgacgaggc gacgcgcgcc ggcgtcgggc tggtgctggg caagccctat
      361 cccgaggatg agctgctcgc gcacgtcgcg agcttcgcgt tcccggccgc gagcgggcgc
      421 gaggccgccg cgggcgcgat cgcctgaagc gcgccggacg ctcgccggcg atacggatgg
      481 ccgtatcgcc tagaggtcga gcttgtgccg cctgtgccac tcggccagcg actccttcgg
      541 gtattcgtcg aaggtctcgt cggccgggcg gacatgggcg tcgacccagt cgggcttgga
      601 gcccaacatc atgtgcgtca cgtcggtcgg ccttggcagc ggcgtgtcga tggcgccggc
      661 gtgcggatgc accagctcgg gccaggtcgg gtcgtagagc cacagcgccg tgccgcagtg
      721 gcggcagaag ttgcgctggc cggtgctgcg ctcgccgccc tccagcctcg cctggtagac
      781 cgagaggttc tcctcgccgc gcaccttcag cgtgcgatgg tcggcgccca ggttgatcgc
      841 gtagccgccc gtggcggcgg tcttgcggca gatcgagcaa tagcagcgca tgaacggcac
      901 cggctcgtgc gacgtcaccg tgaacgtcac cgcgccgcag tggcaggatc cttcgagttg
      961 catcgtggcg tctccggggt gtgggccgcg cgcgcgccgc gcacgcggga tcgggccggg
     1021 gacggcaagg gacgtaccgg ggatcccgcc gagcatgccg ccccgacgcg ccgccgatcg
     1081 cggcgtccga acgcgcgatc ggaagaatcc tgcgggcccc ggtgaaaccg ccgacgaaaa
     1141 cgcgccggcc gggcgcttag gatgcgcgcg gtgatctcca cgcccgaccg cccatgaccg
     1201 acttcgcccc gcccctgccc tcgcccgccg cccgtccctg gtccgccacc gacgacaccg
     1261 ccttcctcgg ccatccgcgc gggctcggct ggctcgcgtt ctccgaactc tgggagcgct
     1321 tttcctacta cggcatgcag acgctgctgg tgctctacat ggtgcacgcg ctgctgctgc
     1381 cgggccatgt cgagcacgtg tggggcttcg acggcttccg gcgcgggctc gagtccgtgt
     1441 acgggccgct gtcggtgcag gcgctggcct cggcgatctt cgggctctac gcgggcaccg
     1501 tctacctcac gccgatcgcc ggcggctgga tcgccgaccg ctggctcggc cgcacgacga
     1561 ccatcacggt cggcgcgctg ctgatggcgc tcggccactt cctgatggcc ttcgaggcca
     1621 gcttcctgat cgccctggcc tgcctgctgc tgggcgtggg cgcgttcaag ggcaacatct
     1681 cgtcgcaggt cggcgcgctc tacggccagg acgacccgcg ccgcgcgacc gcgttccaga
     1741 tcttcctgct ggcggtgcag atcgcggtga tcgtctcgcc ctggctgtgc ggccacctca
     1801 ccgaggacgg caagtactgg gaccgcggct tcggcgccgc cggcgtgggc atggtgatcg
     1861 gcctgctgac ctacctcggc ggccggcgct ggatgccgcc cgaggagcgc atcgccgcct
     1921 ccgctgcggc cgccgtcgag cctcgcccgc cgctgagcgc cgccgaccgc cgcaaggtgg
     1981 ggctgctcgt cctgctgatc ccggtgctgg cgctgacggt ggtgggcaac cagcagatct
     2041 tcaactcgta cctgatctgg gccgagaaga acctcgacct gcacctgttc ggccaggaga
     2101 tcgcggtgac ctcgctgatc tcgctggact ccatcatcag cacggcgacg ctgctcgctt
     2161 cgctgctgtt ctggcatgtc tggcgcaagg tcctgcgcga gcccgacgag ctcatgaaga
     2221 tcacgatggg cgtcgcggtg gccgcctgcg cgccgctgct gctggccggc gcatcggccg
     2281 ccgtgcaggc caccgggcac cgggcgggca tcgggtgggt cgtcgcgtcg cagatcgtca
     2341 acgacttcgg cttcgccaat atcctgccgg tgggcctcgc gctgtactcg cgcgcggcgc
     2401 cgcgcggcat gacgggcacg atggtgggcg tctactacac gcacctgttc ttcgccaaca
     2461 tgggcgtcgg ctggctcggc gggcagtacg agaagatgtc cgcgagcgcg ttctggacga
     2521 tgcacgcggc gatcgtcggc gtgtcggccg tgctgctgct gctcgtgcgg ctcacggtcg
     2581 gccgcggcct gacgccggcc tacgacgcgc cggccgaggc ccgcgcttga agggccgggt
     2641 tcagccgcgc aggccccacg ccacgccgcc cagcacgagc agcccgccca ccgcgtggaa
     2701 ggcgtgcagc ggctcgccca gcagcgccca cgccgccacg gcgaccagca gcggcagcag
     2761 gttgaggaac aggctcgcgc gcggcgcacc gtggtgcttg acgccggtga cccagaagaa
     2821 cggcgccgcg atcgaggccg ggagtgcggc gtaggccacc agcggggcgc tcgcggcggt
     2881 gacgggctgc atgccgcccg cgatccagaa cggcagcacc acgagcgttc cgaagccgat
     2941 ctgcacgtag acctgctgcc aggacgtcag cggcatcgcc cagcgccgca gcagcacgcc
     3001 gtacagcgcg ttggagctca cggccacgag catcagccct tcgccgacgt gcacgccgcc
     3061 cgcggccagc gccgccggat gcccgcgcgt gaccagccag gcgatgccca gcagcgagac
     3121 gatcgccccg cccgcctgcg cgcgcgaggg cgcctccgcc gcgaaggccc gggcgaccag
     3181 cgccgacagc agcggcatcg tggccgtcat cacgcccatg tcgaccgcgc tggcggtgcg
     3241 cgcggcctcg taggcgaggc cctggtaggt gctcatgccc agcgcgccga ggaaggcgat
     3301 ccgcggccag tgcgcgcgca cgacggcgcg ctgccgccac gccgcgcgcg cgaggaaggg
     3361 cgtgagcacg gcgaacgcga tcgcccagcg gaagaaggtg atggcggcgg gctcgatcac
     3421 ggcgctcgcg cgcttggtga ccagcacgtt gcccgtccac acgaggacgg cgaacagcgg
     3481 cgcgagcggt acccaggccg cggtgcgcgg gggcgcggcg gcggcgggtt ggcggagcat
     3541 cggggcggcg gcgtgggtca tcgaggcatg cgggcggagt gcggatgccg tcatcctgca
     3601 gccctgcgag ccgtgcgacc agtgcaatct cggcaagcca cgattgcgtc gcgtgcagct
     3661 tgcgcctgat gccttcgcgc gcgaggatgg cgccatgtcg cgcatcgacc tcaatcaact
     3721 cgccgacctc gacgccctcc ttcgcgaagg cagcgtcgcg cgcgcggccg agcggctgca
     3781 cctgagcgcg cccgcgatga gccgccgcct cgcgcacctg cgccgcacct tcggcgaccc
     3841 gctgttcgtg ctcgctgggc ggcagctcgt gccgaccgag cgcgcactgg cgctgcagcc
     3901 acgcatcgcc gcgctgctgg acgag
//
LOCUS       DASVLN010000096         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_166387, whole genome shotgun sequence.
ACCESSION   DASVLN010000096 DASVLN010000000
VERSION     DASVLN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166387"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VF453_02005"
     CDS             complement(<1..201)
                     /locus_tag="VF453_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7686445.1"
                     /translation="MSGATTCPAVAVLGAGAVGCYYGAMLARAGAPVAMVGRPAHVEA
                     MRRDGLRLTTAAFDEHVPVRADA"
     gene            complement(198..1652)
                     /gene="sbcB"
                     /locus_tag="VF453_02010"
     CDS             complement(198..1652)
                     /gene="sbcB"
                     /locus_tag="VF453_02010"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HEX7686446.1"
                     /translation="MTDFSFYWHDYETFGRMPRRDRPAQFAGVRTDADLNEIGPPAML
                     YCQPAPDTLPDPESCLLTGIVPQTCLERGLPEHAFAARIEAELAAPGTVGVGYNTIRF
                     DDEVTRHLFWRNLIDPYAREWQNECGRWDLLDTVRATWALRPEGIEWPFHEDGRPSFK
                     LEHLTRANGLAHEAAHDALSDVRATIALARLVRSRQPRLWDFCLKLRRKEAVWAEIGA
                     GRPFLHLSGMYGLEQGGMAVVFPLAPHPTNRNELIVWDLTHDPEMLVDLDVDTLRLRL
                     FTRAAELPEGVQRLPVKTIHVNKSPVVVGNLKTLTSAVLDRFALDLGRAQRHAQAAPA
                     IAGRLQQVWREVYARPAADAVPDVDEDLYGGFVGNDDRRKLERLRRMSGEQLAGQRTA
                     FEDGRLEELLFRYRARNFPGTLSADEHARWIDHCAGRLHEGAGGATTLAEFFDRIDQL
                     NEQAEERGDERAAEILGALYDYAEGIAPPPRSAS"
BASE COUNT          234 a          638 c          678 g          234 t
ORIGIN      
        1 ggcatcggcg cgcacgggca cgtgctcgtc gaacgcggcg gtcgtcaggc gcaggccgtc
       61 gcggcgcatc gcctcgacat gcgccggccg gcccaccatg gcgacgggcg cgcccgcgcg
      121 cgcgagcatc gcgccgtagt agcagccgac cgcgcccgcg ccgagcacgg cgaccgcagg
      181 acacgtcgtc gcgccgctca cgaagcgctc cgcggcgggg gcgcgatgcc ttccgcgtag
      241 tcgtacagcg cgcccaggat ctcggcggca cgctcgtcgc cgcgctcctc ggcctgctcg
      301 ttgagctggt cgatgcggtc gaagaactcg gccagcgtgg tcgcgccgcc ggcgccctcg
      361 tgcaggcgcc cggcgcagtg gtcgatccag cgggcgtgct cgtcggcgga gagcgtgccg
      421 gggaagttgc gggcgcggta gcggaacagc agctcctcga gccggccgtc ctcgaacgcg
      481 gtgcgctggc cggccagttg ctcgcccgac atccggcgca ggcgctcgag cttgcggcgg
      541 tcgtcgttgc cgacgaagcc gccgtacagg tcctcgtcga cgtcgggcac cgcgtcggcc
      601 gcgggccgcg catagacctc gcgccagact tgctggagcc ggccggcgat cgcgggcgcc
      661 gcctgcgcgt ggcgctgcgc acggcccagg tcgagcgcga agcgatccag cacggcggac
      721 gtcagcgtct tcaggttgcc gaccacgacg ggcgacttgt tgacgtggat cgtcttgacg
      781 ggcagacgct ggacgccctc cggcagctcg gcggcgcgcg tgaacaggcg caggcgcagc
      841 gtgtcgacgt cgaggtcgac cagcatctcc gggtcgtgcg tgaggtccca cacgatcagc
      901 tcgttgcggt tggtcggatg cggggcgagc gggaagacga ccgccatgcc gccctgctcc
      961 agcccataca tgcccgacag gtgcaggaag ggccggccgg cgccgatctc ggcccacacg
     1021 gcctccttgc ggcgcagctt caggcagaaa tcccacagcc gcggctggcg cgacctcacc
     1081 agccgcgcga gcgcgatggt cgcgcgcacg tcggacagcg cgtcgtgcgc cgcctcgtgc
     1141 gcgaggccgt tggcgcgggt caggtgctcg agcttgaacg acggccggcc gtcctcgtgg
     1201 aacggccact cgatgccttc gggccgcaag gcccaggtag cccgcaccgt gtccagcagg
     1261 tcccagcggc cgcactcgtt ctgccattcc cgcgcgtagg ggtcgatcag gttgcgccag
     1321 aacaggtggc gcgtgacctc gtcgtcgaag cggatcgtgt tgtagccgac gcccaccgtc
     1381 cccggcgccg cgagctcggc ctcgatgcgc gccgcgaacg cgtgctcggg caggccccgc
     1441 tcgaggcagg tctgcgggac gatgcccgtg agcaggcagg actcgggatc gggcagcgtg
     1501 tccggcgcgg gctggcagta cagcatcgcc ggcgggccga tctcgttgag gtcggcgtcg
     1561 gtgcgcacgc ccgcgaactg cgcggggcgg tcgcggcggg gcatgcggcc gaaggtctcg
     1621 tagtcgtgcc agtagaagct gaaatcggtc atgccctgca agatacccga gaaagcgcgg
     1681 gccgcccggc gcgcaatgcc gcgttttcgg ccctcccggc gcgggggggg gcgcggccgg
     1741 ccgccggctt caggcggccg cgggcacgac cggcggcagc agcc
//
LOCUS       DASVLN010000097        10892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_434819, whole genome shotgun sequence.
ACCESSION   DASVLN010000097 DASVLN010000000
VERSION     DASVLN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434819"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VF453_02015"
     CDS             complement(<1..753)
                     /locus_tag="VF453_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX7686447.1"
                     /translation="MLEDQPEDVEEGARQSAFRTGYRTGFADGFDAADKVHEASRRKS
                     GKDDGEDKDGDGKPGGRQDAGQGDDDPSRDGGRNGKDDKHDKGGEKDEDKPKGPPLWK
                     RPGVVLVIIVMAIAAIVGVTLFVIHSRSHETTDDAFIDGNASQIAAQAAGRVTALHVN
                     DNQEVKAGDPLLDIDTADVQAKVDQAKAQVLSAQGQESQARAQVETQKASAAQASAQV
                     LAAQAEATNAHQDLARFKGVDPDAVSRQQYDQA"
     gene            complement(793..1512)
                     /locus_tag="VF453_02020"
     CDS             complement(793..1512)
                     /locus_tag="VF453_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686448.1"
                     /translation="MSQPFETWQVLPHGQLTEVDVNLLTVVGQIEMPLGEFPRRMTVA
                     RLRDGRLVVYSAIALDDDEMAALERWGRPAFLVVPGDHHRMDVKIWKQRYPSMEVAAP
                     AGARVMVEQVVPVDAVSPDFGDPDVQWITVPGTREHEAALLVRGRDGTTLVLNDIVAN
                     IRHEHGFGGWLMRMMGFAVDEPNIPGVRKLAMIKDAEMLRDQLLAWSEIADLRRILVS
                     HGEPIVDDPRGVLRKLAGSLR"
     gene            1687..2037
                     /locus_tag="VF453_02025"
     CDS             1687..2037
                     /locus_tag="VF453_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEX7686449.1"
                     /translation="MTILYGIPNCDTVKRARAWLADRGVGVEFHDFKKAGVPADRLAR
                     WVEAAGWERVLNRQGTTWRKLDPAVQAAVVDAASAQALMREQASVIKRPVVEWDDGRI
                     TVGFDEKAWAAGLE"
     gene            complement(2068..3396)
                     /locus_tag="VF453_02030"
     CDS             complement(2068..3396)
                     /locus_tag="VF453_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX7686450.1"
                     /translation="MAWTFSLPRFATAPFCPSEVQGSIAVAPGLPLWQRVLRFAGPGL
                     LVAVGYMDPGNWATDIEAGSVFGNSLLFVVLLSSVAAIVLQVLALRLGIVARMDLARA
                     CRERYGPPVRVALWLMAEVAIVACDVAEVLGSALALHLLFAMSLVTGIVVTAFDTLLV
                     LGLKGQGFRQVEAIVLALVATIGVCFAIELGLVGPDWHAVAAGFVPHTDTLRDPHALY
                     VAIGILGATVMPHNLYLHSSIVQTRRIGRADADRAAALRLSTVDTVGSLVLALLVNAA
                     ILVLAARAFHGTGHAGVTEIQDAYHLLDPIVGTGAASLLFGVALLASGQSSTFTGTIA
                     GQVMMEGFLKLSIPCWQRRLITRGLALVPALAGVLWLGESGVGTLLVASQVVLSLQLP
                     FAIWPLVRLTSDRAVMGRFANGAALRASAWTLFAAITVANAWLLAGLLRG"
     gene            complement(3542..4159)
                     /locus_tag="VF453_02035"
     CDS             complement(3542..4159)
                     /locus_tag="VF453_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686451.1"
                     /translation="MSLENAYRKLLAVDPGNLPSECRQLMFVQPAHVLPVSPDLQIVT
                     WAALREAAAAHDLFPKLGIKSWRDSGSSRLIARGMPMRPVIEAILSIPTWQQRTLMTA
                     WTVTGAQLNLYLYPFVDFTDISESRWLASAHGCRFVSGCQRGRSAPLFGAALRNMQRL
                     AAQIAQAMGDDPQVVELGMLPSGEVRLVEINPALTPAECKALMNT"
     gene            complement(4156..4671)
                     /locus_tag="VF453_02040"
     CDS             complement(4156..4671)
                     /locus_tag="VF453_02040"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX7686452.1"
                     /translation="MIQVSDDIKQGWLYYPPRKANDGDILSLETAFGASLPKQYIDFV
                     KTYGFVKFGYDANDTRIATVIYEGEGQRELRQATIGFLVTPERAIQTWTTFTDKSDPD
                     AEDLPLIPDGYFPAGNDNGQGLVLIELATGRIWYWRESAWRWGTEDNTRLGLLAEDFD
                     GFINALRPETA"
     gene            complement(4681..5586)
                     /locus_tag="VF453_02045"
     CDS             complement(4681..5586)
                     /locus_tag="VF453_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686453.1"
                     /translation="MTEKFNSALSEQLRSNVWKRTQEAVANMSPTEGAGLVADVTGIF
                     DPTPASDTVGLALSLVQGDGVGAVASLVSMFPYVGDAVAKPFKIARRAPKVAEAIEAV
                     LKGGDRLAGAGKAALKDAGLSLDQVAAARKQALQTVQKAMVEAKQKIANCETCKLKGA
                     DGSRAGVLHMPQNGKNGKWASGSQPSDGNGLFEFTQPKTLPDGRQVASVEYRDGAPVL
                     DDFVLGQKHELWEVTGDVGKDETQLKAMMRQTDPAWRPPDPETYVLHHFEDGAVGYVP
                     RVIHDKAIGGVAHTGGNSMLNTELF"
     gene            complement(5579..5992)
                     /locus_tag="VF453_02050"
     CDS             complement(5579..5992)
                     /locus_tag="VF453_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686454.1"
                     /translation="MVLRFTTAQIDAPVLADPRLLERELFMHLLEVRPNLRACYPLPY
                     LRWVVRDSIRLAAPFRFDTVAAYRMFLLLRFDVAPGYFKQSDIAQALSDTSLETMERW
                     KRLTGPEYADAWLQAYAFDGAGEWRATFWNEPAHD"
     gene            6161..6877
                     /locus_tag="VF453_02055"
     CDS             6161..6877
                     /locus_tag="VF453_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase subunit alpha"
                     /protein_id="HEX7686455.1"
                     /translation="MFTGIIQAVATVSAVTDRPGLRSFTLAFPRGFCEGLEIGASVAV
                     DGVCLTVTERVGEDAANFDVMQQSLNLTTLGTLAQGSRINVERAARDGAEIGGHPLSG
                     HVDFQATIAQVRTPENNHVLRIAVPPQWMRYVFPKGYIAVNGASLTVAEADRAAGWFE
                     VWLIPETLRMTTFADKREGAALNIEIERGTQVMVDTVRAAVDERLGPLMPALIELLRE
                     RGTDVDALLGADVVARLPRA"
     gene            6874..7581
                     /locus_tag="VF453_02060"
     CDS             6874..7581
                     /locus_tag="VF453_02060"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7686456.1"
                     /translation="MTIVPVTGPAPPPRLVPDADPYRPPPDTGLEVVHEEAGFVVLAK
                     PPGLLSEPGRGPDKRDSLVVRAQARWPWARLVHRLDMMTSGLIVVALDEAVHSEFNRL
                     FRERLVRKRYEALVHGRPAAEAGAIDLPLAVDWPNRPRQVVDLERGKPSLTRYRVTGD
                     VPGGLARVALEPVTGRTHQLRVHLAALGHPIAGDPFYGIAAGADAPRMMLHACGIEFP
                     HPAGDGRDCRFDVAVPF"
     gene            7685..8806
                     /locus_tag="VF453_02065"
     CDS             7685..8806
                     /locus_tag="VF453_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7686457.1"
                     /translation="MPQAVITGTGLYQPPHVVTNAELVAAFNAYADAQNAKNAEAIAR
                     GEAVAMTASSVEFIEKASGIKQRYVIEKTGVLDPARMRPKFEQRPDDQLSLMAEIAVD
                     AARKALAAAGRQAADIDAVLCAASNMQRAYPAMAVEIQQALGAGGYGFDMNVACSSAT
                     FAIEQAVNAVRAGSAKCVLVVNPEITSAHLEWKDRDCHFIFGDVCTATLVESADTAVS
                     SDRWEVLGTRLATQFSNAIRNNFGFINRSEDADPLARDKTFRQEGRKVFKEVVPLAAA
                     HIEAHLAAQGLEPTQIRRFWLHQANLGMNQLILKKLVGADAGTDVAPLVLDTYANTSS
                     AGSVLAFHEYRADLQAGDTGVICSFGAGYSIGSVIVRKQ"
     gene            complement(8816..9649)
                     /locus_tag="VF453_02070"
     CDS             complement(8816..9649)
                     /locus_tag="VF453_02070"
                     /inference="COORDINATES: protein motif:HMM:NF038119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHFG family PEP-CTERM protein"
                     /protein_id="HEX7686458.1"
                     /translation="MGMSLIVAAAMASATGSGCSWAHPGANPYRGAPEAVLADFAMPD
                     DTRRQLRALMVARRYTDVVTITRDRIQGQHVYTDLRQMHSGHGVVCHGSVDRSAWSAK
                     HEERGLVYCVGDTCVVVPTVCNNVSLLTRKPEDEPLQSGEDESIDVTPAAGAAPPPAD
                     TTVAQGPDDSPLSFEPPAAGMPGSTDQPPPDDGGGDGGGPSGFPSTPLGGGGGDVIGC
                     CGTVPAGPGGPVTPPVTPPVTPPVTPPVVPPTSPVPDAPEWSLLLAGLAVIAAVARRR
                     R"
     gene            complement(9911..>10892)
                     /locus_tag="VF453_02075"
     CDS             complement(9911..>10892)
                     /locus_tag="VF453_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7686459.1"
                     /translation="ELRCEAACRERDAAAGAIMVAPVADVEPAESAAGRVERSLARLN
                     ALRTASGTHPVARSELLSLSASRHAAYLSANGFRSAPSVHAEAAGLGYFTGADPFSRM
                     RAAGYHSTYATEVVGDIGSVATDSDCVDHLMGTVYHATLLLSRVTEAGMAYGSGAAAG
                     ACVIDLGVPLLAPGPEAAATGRLVRYPWAGMTAPTGSLRLDRETPRPPQALLPDARVG
                     IPVLVGLRDAASVMGGSGAPDVRILDFELHDARDMPVPSVILADPAISGPGLVADAAV
                     RGGFAVLIPRKPLAPGRYRVQLRATVGADVIAPAPWTFDVASDASSGAAH"
BASE COUNT         1662 a         3856 c         3739 g         1635 t
ORIGIN      
        1 cgcctggtcg tattgctggc gcgagaccgc gtccgggtcg acgcccttga agcgcgcgag
       61 atcctggtgc gcgttggtcg cctcggcctg cgcggcgagc acctgcgccg aggcctgcgc
      121 cgcgctcgcc ttctgcgtct cgacctgcgc ccgggcctgc gactcctggc cctgcgcgct
      181 cagcacctgc gccttggcct ggtcgacctt ggcctggacg tcggcggtgt cgatgtccag
      241 cagcggatcg cccgccttga cctcctggtt gtcgttcacg tgcagcgcgg tcacacggcc
      301 cgcggcctgc gccgcgatct ggctggcgtt gccgtcgatg aaggcgtcgt cggtggtctc
      361 gtgcgagcgc gaatggatga cgaacagcgt gacgccgacg atggccgcga tcgccatgac
      421 gatgatcacg agcacgacgc ccgggcgctt ccacagcggc gggcccttgg gcttgtcctc
      481 gtccttctcg ccgcccttgt cgtgcttgtc gtccttgccg ttcctgccgc cgtcgcggga
      541 cgggtcgtca tcgccctggc cggcgtcctg cctgccgccg ggcttgccgt cgccgtcctt
      601 gtcctcgccg tcgtccttgc cggactttcg gcggctcgcc tcgtgcacct tgtcggcggc
      661 gtcgaagccg tcggcgaacc cggtgcggta tccggtacgg aaggccgact ggcgtgcgcc
      721 ttcctcgacg tcctcgggct ggtcttcaag catcgtgcgg gatcctcgcg tggtcgtgcg
      781 tgttcgcttc cctcatcgca gcgaccccgc gagcttgcgc agcacgccgc gcgggtcgtc
      841 cacgatcggc tcgccgtgcg agacgaggat gcgcctcagg tcggcgatct cgctccatgc
      901 gagcagctgg tcgcgcagca tctcggcgtc cttgatcatc gcgagcttgc gcacgccggg
      961 gatgttgggc tcgtcgaccg cgaagcccat catgcgcatc agccagccgc cgaagccgtg
     1021 ctcgtggcgg atgttggcga cgatgtcgtt gagcaccagc gtcgtgccgt cgcggccgcg
     1081 caccagcagc gcggcctcgt gctcgcgcgt gccgggcacg gtgatccact gcacgtcggg
     1141 atcgccgaag tccggcgaga cggcgtcgac cggcacgacc tgctcgacca tcacccgcgc
     1201 accggccggc gccgcgacct ccatcgaggg atagcgctgc ttccagatct tcacgtccat
     1261 gcggtggtgg tcgcccggca cgacgaggaa cgcggggcgg ccccagcgct ccagcgccgc
     1321 catctcgtcg tcgtcgagcg cgatggcgct gtagacgacc agccgcccgt cgcgcagccg
     1381 cgccaccgtc atgcgacgcg ggaactcgcc cagcggcatc tcgatctggc cgacgacggt
     1441 cagcaggttg acgtcgactt ccgtcaactg gccatgcggc aagacttgcc acgtctcgaa
     1501 cggctgggac atgccggctc cggggatgga cggcgcggga aggcgcgcgg acacgacgtt
     1561 ccggcacgcg cacggccggg cgtcgacgag cccgcaggtc ggcaatcgcc atgccggcgc
     1621 ggcgcgaacg ctcggttgcg gcggacacgc gcgcgcgggc ggccgtggcg cgcgacaatg
     1681 gccgccatga cgattctcta cggcattccc aactgcgaca ccgtcaagcg cgcccgcgcg
     1741 tggctggccg accgcggcgt cggcgtcgag ttccacgact tcaagaaggc cggcgtgccc
     1801 gccgatcgcc ttgcccgctg ggtcgaggcc gcgggatggg agcgcgtgct caatcgccag
     1861 ggcacgacct ggcgcaagct cgacccggcg gtgcaggccg cggtcgtcga cgccgcgtcc
     1921 gcgcaggcgc tcatgcgcga gcaggcgagc gtcatcaagc ggccggtcgt cgaatgggac
     1981 gacggacgca tcaccgtcgg cttcgacgag aaggcctggg ccgccggcct cgagtgaggc
     2041 cgcgtccggg caggggcggc ccgcgcgtca cccgcgcagc aggcccgcca gcagccatgc
     2101 gttggcgacg gtgatggcgg cgaacagcgt ccaggccgag gccctcaggg cggcgccgtt
     2161 ggcgaagcgg cccatcaccg cgcggtcgct cgtgagccgg accagcggcc agatcgcgaa
     2221 cggcaactgc aggctcagca cgacctggct cgcgaccagc agcgtgccga cgccggactc
     2281 gcccagccac agcacgccgg ccagcgccgg cacgagcgcg aggccgcgcg tgatgaggcg
     2341 ccgctgccag cacgggatcg agagcttcag gaagccttcc atcatcacct ggccggcgat
     2401 cgtgccggta aaggtcgagc tctgccccga cgccagcagc gcgacgccga acagcagcga
     2461 cgccgcgccc gtgccgacga tggggtcgag caggtggtat gcatcctgga tctcggtcac
     2521 gcccgcgtgg ccggtgccgt ggaaggcgcg cgccgcgagc acgaggatcg ccgcgttgac
     2581 gagcagcgcc agcaccagcg agccgaccgt gtcgacggtc gacaggcgca gcgcggcggc
     2641 gcggtcggcg tcggcgcggc cgatgcggcg cgtctgcacg atggacgagt gcaggtacag
     2701 gttgtgcggc atcacggtcg cgccgaggat cccgatcgcg acgtagagcg cgtgcgggtc
     2761 gcgcagcgtg tccgtgtgcg gcacgaagcc ggcggccacc gcgtgccagt cggggccgac
     2821 gaggcccagc tcgatcgcga agcacacgcc gatcgtcgcg accagcgcga gcacgatggc
     2881 ctcgacctgg cggaagccct ggcccttcag gcccagcacc agcagcgtgt cgaaggcggt
     2941 cacgacgatg cccgtcacca gcgacatcgc gaacagcagg tgcagcgcga gcgcgctgcc
     3001 cagcacctcc gcgacgtcgc acgcgacgat cgcgacctcg gccatcagcc acagcgcgac
     3061 gcgcacgggc ggcccgtagc gctcgcggca cgcgcgcgcc aggtccatgc gcgcgacgat
     3121 gcccaggcgc agcgccagca cctgcagcac gatcgccgcg acgctggaca gcagcaccac
     3181 gaacagcagc gagttgccga acaccgagcc ggcctcgatg tccgtggccc agttgcccgg
     3241 atccatgtag ccgacggcga ccagcagccc cggcccggcg aagcgcagca cgcgttgcca
     3301 cagcggcagg ccgggcgcga cggcgatgct gccctggacc tcggacgggc agaacggcgc
     3361 ggtggcgaag cggggcagcg agaaggtcca ggccatggcc cgattgtccc gcccggccgc
     3421 ggcgtgcgcg cggcgcgggc cattggacga tgcaatggcc gcgttagcta gactgtaccc
     3481 acagtgcttt tcgcagcatg cggacgaggc gcctcgaccg gtccaccgcc gacggcggac
     3541 gtcaggtgtt catcagtgcc ttgcactccg ctggcgtcaa agcggggttg atctcgacca
     3601 gacggacctc gcctgagggc agcattccaa gttccacgac ctgcggatcg tcgcccattg
     3661 cctgcgcgat ttgcgcggcg aggcgctgca tattgcgcag tgcggcgccg aacagcggcg
     3721 cggagcggcc tcgttggcag ccactgacga accggcagcc atgagcgctg gccagccatc
     3781 gtgattcgct gatgtcggtg aagtcgacga agggatagag ataaaggttc agttgtgcgc
     3841 ctgtgaccgt ccacgcggtc atcaacgtgc gctgctgcca tgtcggaatc gacaaaatgg
     3901 cttcgatcac cggccgcatc ggcattccgc gcgcgatgag gcgagaactg ccgctgtcac
     3961 gccagctctt gatgcccagt ttgggaaaca agtcgtgcgc cgccgcggct tcacgcaggg
     4021 ccgcccaagt cacgatctga agatccgggc tgaccggcag cacatgtgcc ggctggacga
     4081 acatcagttg acggcactcg ctaggcaggt tgccggggtc gactgcgagc agcttccggt
     4141 aggcgttctc aaggctcatg ccgtttcggg acgcagagcg ttgatgaagc cgtcgaaatc
     4201 ttccgcgaga aggcccagtc tggtgttgtc ctccgtgccc cagcgccatg cgctctcgcg
     4261 ccagtaccag attcgcccgg tcgccagctc gatgaggacc aggccctgtc cgttgtcgtt
     4321 gcccgccggg aagtagccgt cgggaatcag cggtagatct tctgcgtccg ggtcgctctt
     4381 gtcagtgaag gtcgtccagg tctgtatggc tcgctcaggc gtgacgagaa agccgatggt
     4441 tgcctgacgc agctcgcgct gaccctcgcc ttcgtagatg acggtagcga ttcgcgtgtc
     4501 gttggcgtcg tagccgaact tcacgaagcc ataggtcttc acgaagtcga tgtactgctt
     4561 cggcaaggac gcgccgaagg cggtttccag cgaaaggatg tccccgtcat tcgcctttct
     4621 ggggggatag tagagccagc cttgcttgat gtcgtcggag acctgaatca tcgaagcacc
     4681 tcagaagagt tctgtgttga gcatggagtt tccgcccgta tgagcgacgc caccaatcgc
     4741 cttgtcgtga atcactcgtg gcacgtagcc caccgcgccg tcctcgaaat gatgaaggac
     4801 ataggtttcg gggtccggtg gccgccaggc cggatcggtc tggcgcatca tggccttcag
     4861 ctgtgtctcg tctttgccaa catcaccggt cacttcccag agctcatgct tctgacctaa
     4921 gacgaagtcg tccagaacgg gcgcgccgtc tcgatactcc accgaggcca cctggcgtcc
     4981 atcggggagt gtcttcggct gggtgaattc gaacagcccg ttgccgtccg aaggctggga
     5041 tccgctggcc cattttccat tcttgccatt ctgtggcatg tgcaagaccc cggcgcgact
     5101 gccgtcggca cccttcagct tgcaggtctc acagttcgcg atcttctgct tcgcctcgac
     5161 catggccttc tgcacggtct gaagcgcctg cttgcgggct gctgcgacct ggtcaagcga
     5221 cagacccgca tccttcaagg cggcttttcc ggcgccggcc agcctgtcgc cgcccttgag
     5281 tacggcttcg atcgcctccg caaccttggg cgctcggcgc gcaatcttga acggcttggc
     5341 gaccgcatca ccgacgtatg gaaacatgct caccagcgat gcgaccgcac caaccccatc
     5401 gccctgaact agggacagtg cgagtccgac cgtatccgag gcgggtgtcg ggtcaaatat
     5461 tcccgtcaca tctgccacca atccagcgcc ctcggtcggg gacatgttgg ccaccgcctc
     5521 ctgcgtgcgc ttccacacat tcgatctcag ttgctcggac agggccgagt tgaatttctc
     5581 agtcatgcgc gggttcattc caaaatgttg cacgccattc accggcgcca tcgaaagcgt
     5641 aggcctgaag ccaggcgtct gcgtactccg gtccggtcag gcgcttccag cgctccatcg
     5701 tctccaggct ggtgtcggac aaggcctgtg caatatcgct ctgtttgaaa tacccgggcg
     5761 ccacatcaaa tcgcagcagc aggaacattc gataagcggc gactgtgtcg aaccggaacg
     5821 gagcagcaag cctgatgctg tcacgcacga cccagcgcag gtatggaagg gggtagcacg
     5881 cacgcaagtt cggcctgacc tccaggaggt gcatgaacag ttcgcgctcc agcagtcgtg
     5941 gatccgccag gactggagcg tcgatctgag cagtggtgaa tctgagtacc atgtggtcat
     6001 gttagacgtt ggctccggcg cagttcagtg aggccgagcc cgtgtcaggt cgacctgcgg
     6061 tacgacgagt gcgaccggct gtctcggacg cccgacgttc gcccgccgcc atgcccttga
     6121 ggcgacaata cgggcttcca ccgctccgag ctcgcctacc atgttcaccg gcatcatcca
     6181 ggccgtcgcc accgtctccg ccgtcaccga ccgccccggg ctgcgcagct tcacgctcgc
     6241 gttcccgcgc ggcttctgcg aggggctcga gatcggcgcg agcgtcgcgg tcgacggcgt
     6301 gtgcctgacc gtcaccgagc gcgtcggcga ggacgcggcc aacttcgacg tgatgcagca
     6361 gagcctgaac ctcacgacgc tgggcacgct ggcgcagggc tcgcgcatca acgtcgagcg
     6421 cgcggcgcgc gacggcgcgg agatcggtgg ccatccgctg tccggccacg tggacttcca
     6481 ggccacgatc gcccaggtgc gcactcccga gaacaaccac gtcctgcgca tcgccgtgcc
     6541 gccgcaatgg atgcgctacg tctttcccaa gggctacatc gccgtcaacg gcgcgagcct
     6601 cacggtggcc gaggccgacc gcgcggcggg ctggttcgag gtctggctga tcccggagac
     6661 gctgcgcatg acgaccttcg ccgacaagcg cgagggcgcg gcgctcaaca tcgagatcga
     6721 gcgtggcacg caggtgatgg tcgacacggt gcgcgccgcc gtcgacgagc gcctcggccc
     6781 gctgatgccc gcgctgatcg agctgctgcg cgagcgcggc accgacgtcg acgcgttgct
     6841 gggcgcggac gtcgtcgccc ggctgccgcg ggcgtgacga tcgtccccgt caccggtccg
     6901 gcgccgccgc cccgcctggt gccggacgcc gatccctacc ggccgccgcc cgataccggc
     6961 ctggaggtgg tgcacgagga ggccggcttc gtcgtcctcg ccaagccgcc gggcctgctc
     7021 agcgagcccg ggcgtggccc cgacaagcgc gacagcctcg tcgtgcgcgc acaggcgcgc
     7081 tggccgtggg cgcggctcgt gcaccggctc gacatgatga ccagcgggct gatcgtcgtc
     7141 gcgctcgacg aggccgtgca ctcggagttc aaccgcctgt tccgcgaacg cctcgtccgc
     7201 aagcgctacg aggcgctcgt gcatggacgt cccgcggccg aggccggcgc gatcgacctg
     7261 ccattggccg tcgactggcc caaccggccg cgccaggtgg tcgacctcga gcgcggcaag
     7321 ccttcgctca cccgctaccg cgtgaccggg gacgtgcccg gcgggctcgc acgcgtcgcg
     7381 ctcgagcccg tcaccggccg cacgcaccag ctgcgcgtgc acctggccgc gctcggccat
     7441 ccgatcgccg gcgatccgtt ctacggcatc gccgccgggg ccgacgcgcc gcgcatgatg
     7501 ctgcacgcgt gcggcatcga gttcccgcat ccggccggcg acgggcgcga ctgccgcttc
     7561 gacgtcgccg tgccgttctg agtcgcgcag aagtaccggc gcgccccgga tttccccggg
     7621 gatggccctc tacgaaacga gtaacacgct gggtacactg cccacggttc acccaggatt
     7681 tcccatgccc caagccgtca tcacgggaac cggcctctac cagccgcccc acgtcgtcac
     7741 caacgcggaa ctcgtcgccg ccttcaacgc ctacgccgac gcgcagaacg cgaagaacgc
     7801 cgaggccatc gcgcgcggcg aggcggtcgc gatgacggct tccagcgtcg agttcatcga
     7861 gaaggcctcg ggcatcaagc agcgctacgt gatcgagaag acgggcgtgc tcgacccggc
     7921 ccgcatgcgc ccgaagttcg agcagcgtcc cgacgaccag ctttcgctga tggccgagat
     7981 cgccgtcgac gccgcgcgca aggcgctcgc cgcggccggc cgccaggccg ccgacatcga
     8041 cgccgtgctc tgcgccgcct ccaacatgca gcgcgcctac ccggcgatgg ccgtcgagat
     8101 ccagcaggcg ctcggcgccg gcggctacgg cttcgacatg aacgtggcgt gctcgtcggc
     8161 caccttcgcg atcgagcagg ccgtcaacgc cgtgcgcgcg ggcagcgcga aatgcgtgct
     8221 ggtggtcaat ccggagatca cctcggccca cctcgagtgg aaggatcgcg actgccactt
     8281 catcttcggc gacgtctgca cggcgacgct cgtcgagagc gccgacactg ccgtctcgag
     8341 cgaccgctgg gaggtgctcg gcacccgtct cgcgacccag ttctccaacg ccattcgcaa
     8401 caacttcggc ttcatcaacc gcagcgagga cgccgatccg ctcgcgcgcg acaagacctt
     8461 ccgccaggaa ggccgcaagg tgttcaagga agtcgtgccg ctggccgccg cgcacatcga
     8521 ggcccatctc gcggcgcagg ggctggagcc cacgcagatc cgccgcttct ggctgcacca
     8581 ggccaacctc gggatgaacc agctgatcct gaagaagctc gtcggcgccg acgccggcac
     8641 ggacgtcgcg ccgctggtgc tcgacaccta cgccaacacc tcgtcggccg gctcggtgct
     8701 cgcgttccac gagtaccgcg cggacctgca ggccggcgac accggcgtga tctgctcgtt
     8761 cggcgccggg tactcgatcg gcagcgtgat cgtgcgcaag cagtgaggcg cgcggtcagc
     8821 ggcgccggcg ggccaccgcg gcgatcacgg ccaggccggc gagcaggagc gaccactcgg
     8881 gcgcgtccgg caccggtgaa gtcggcggca cgaccggcgg cgtgacgggc ggcgtcacgg
     8941 gaggcgtgac cggcggcgtg accggtccgc ccggcccggc gggcacggtg ccgcagcagc
     9001 cgatgacatc gccgccgccg ccgccgaggg gcgtggaggg gaagccgctt gggccgccgc
     9061 catcgccacc gccgtcgtcc gggggcggct gatccgtcga accgggcatc cccgcggcgg
     9121 gaggttcgaa ggacagcggg ctgtcgtccg ggccctgcgc cacggtggtg tccgcgggcg
     9181 gcggggcggc gcctgccgcg ggcgtgacgt cgatcgattc gtcctcgccc gattgcagcg
     9241 gttcgtcctc cggcttgcgc gtgagcagcg agacgttgtt gcacacggtg ggcaccacca
     9301 cgcaggtgtc gccgacgcag tacacgaggc cgcgctcctc gtgcttcgcg ctccaggcgg
     9361 agcgatcgac gctgccgtgg cagaccacgc cgtggccgct gtgcatctgg cgcaggtccg
     9421 tgtagacgtg ctggccctgg atgcggtcgc gcgtgatggt gaccacgtcc gtgtagcggc
     9481 gcgcgaccat cagcgcccgc agctgccgcc gcgtgtcgtc cggcatcgcg aagtcggcca
     9541 gcaccgcttc cggcgccccc cggtacggat tggcgcccgg atgggcccac gaacatccgc
     9601 tccccgtcgc cgacgccatg gccgcggcca cgatcaggct catccccatt ggaggtctcc
     9661 caagtgtcgc ggggcgtgac atccaggcca cggtgcccga ccgactgacg tttgttgttc
     9721 ggttccgcaa tcacttgcgg cgcgcgcgag tctacggggc gctgggagcc gaccgaatga
     9781 ccctgctgca gtcgagcagg aaatttgtgt caagtgtcac gaaaagttat cggtttggcg
     9841 caacacgacg actgcgaaca atcggaaatg cgctaaggcg gcgccgggcg cgagacgagc
     9901 cgcggcggct tcagtgcgcg gcgcccgagg acgcatcgga cgccacgtcg aacgtccagg
     9961 gagcgggcgc gatcacgtcg gcgccgacgg tcgcgcgcag ctgcacccga tagcgccccg
    10021 gcgccagcgg cttgcgcggg atgaggacgg cgaagccccc gcggacggcg gcgtcggcca
    10081 cgaggcccgg ccccgagatc gccggatccg cgaggatcac gctggggacg ggcatgtccc
    10141 gcgcgtcgtg cagctcgaag tccaggatcc ggacgtcggg cgccccggag ccccccatga
    10201 cggaggccgc atcgcgcagc cccaccagca cggggatgcc gaccctcgca tcgggaagca
    10261 gcgcctgcgg cgggcgtggc gtctcccgat ccaggcgcag ggagccggtc ggcgccgtca
    10321 tcccggccca gggatagcgc acgagccggc cggtcgcggc ggcctccggc cccggcgcga
    10381 gcagcggcac gccgaggtcg atcacgcacg cccccgcggc cgcgccgctg ccgtaggcca
    10441 tgcccgcctc cgtcacccgg ctcagcaaca gcgtggcgtg gtagaccgtg cccatcaggt
    10501 ggtcgacgca gtcgctgtcg gtcgccaccg agccgatgtc gccgacgacc tccgtggcat
    10561 acgtcgagtg gtacccggcc gcccgcatgc gggagaaggg atcggcgccg gtgaagtacc
    10621 cgagccccgc ggcctccgcg tgcaccgagg gcgccgagcg aaagccgttg gcgctgaggt
    10681 aggccgcatg ccgggacgcc gagagggaaa gcagctccga gcgcgcgacc ggatgcgttc
    10741 ccgacgcggt gcgaagggcg ttcaggcgcg cgagcgaacg ctccacgcgg ccggcggcgg
    10801 actccgcggg ctcgacgtcg gcaaccggcg ccaccatgat cgcgcccgcg gcggcgtcgc
    10861 gttcgcggca ggccgcctcg cagcggagct ct
//
LOCUS       DASVLN010000098         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_326981, whole genome shotgun sequence.
ACCESSION   DASVLN010000098 DASVLN010000000
VERSION     DASVLN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326981"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF453_02080"
     CDS             complement(<1..318)
                     /locus_tag="VF453_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7686460.1"
                     /translation="METLALIELLDRDGQARQATRVSAWPVRVGRALDSDVVLDDPHV
                     AAHHLELVERDGIVQVVPASTINGVRVGRATVAAGTAATLPASGLLHLGPLTLRVRLA
                     SAAL"
     gene            complement(300..>1603)
                     /locus_tag="VF453_02085"
     CDS             complement(300..>1603)
                     /locus_tag="VF453_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX7686461.1"
                     /translation="SAPAALLPQAAASGPAKAESDRAAMSAPARRIYDDGRPRLLQVR
                     TLLRDQDSQASVGSGSLVGPGGLILTNWHVVSQAALEPQRYRLSYQMVDGASGALQLV
                     AFDVVHDLALVRMAAPPADVARRGLFQIRPDTGAALAQGERIFSLGNPLDVGFVVVEG
                     TYNGLVERSFLPQILFSGSLSPGMSGGPVVDDHGLIVGVNDATRLDGQQVSFLVPAEF
                     AKALIARGASSRPITGSVDAELARQLLVHQQSLTERFVALPWRQIKHAHYDVPVPQED
                     FMRCWGSSNPVAAAGLQFERSDCQMDTAIFISQSLRTGDLSVRHEVYDGSKLGPLRFA
                     QEYSLSFQNENMGHRTHDLTAPQCKEGFVENGGLPMRTVMCLRAYRRLPGLYDVAVLT
                     TSVDAKTEGVQGRFDAHGVSFANAQLLARHYLQGFAWKRSP"
BASE COUNT          229 a          577 c          587 g          210 t
ORIGIN      
        1 gagcgccgcg ctcgcgagcc gcacccgcag cgtcagcggc ccgaggtgca gcaggcccga
       61 agcaggcagc gtcgcggcag tgccggcggc caccgtcgcg cggccgacgc gcacgccgtt
      121 gatcgtcgag gccggcacga cctgcacgat gccgtcgcgc tcgacgagtt ccaggtggtg
      181 cgcggcgacg tgcgggtcgt cgagcaccac gtccgagtcc agcgcccggc cgacgcgcac
      241 cggccacgcc gacacgcgcg tcgcctggcg cgcctggccg tcgcgatcca gcagctcgat
      301 cagggcgagc gtttccatgc gaatccctgc aggtagtggc gggccagcag ctgggcattg
      361 gcgaagctca cgccatgggc gtcgaagcgg ccctgcacgc cctcggtctt cgcgtcgacc
      421 gaggtcgtga gcacggcgac gtcgtacagc ccgggcagcc ggcggtaggc gcgcaggcac
      481 atcaccgtgc gcatcggcag cccaccgttc tccacgaagc cctccttgca ctgcggcgcc
      541 gtcaggtcgt gcgtgcggtg gcccatgttc tcgttctgga agctgaggct gtattcctgc
      601 gcgaaccgca gcgggcccag cttgctgccg tcgtagacct cgtggcgcac cgacaggtcg
      661 ccggtgcgca gcgactggct gatgaagatc gccgtgtcca tctggcaatc cgaacgctcg
      721 aactgcagcc ccgccgcggc caccgggttg gacgagcccc agcagcgcat gaagtcttcc
      781 tgcggcaccg gcacgtcata gtgcgcgtgc ttgatctggc gccacggcag cgcgacgaag
      841 cgctcggtca gcgactgctg gtgcacgagc agctgccgag cgagctcggc atcgacgctg
      901 cccgtgatcg gcctggacga cgcgccgcgc gcgatgagcg ccttcgcgaa ctcggccggc
      961 acgaggaagc tgacctgctg gccgtcgagc cgcgtcgcgt cgttgacgcc gacgatcagg
     1021 ccgtggtcgt cgaccaccgg ccctccgctc atgcccgggc tgagcgagcc cgagaacagg
     1081 atctgcggca ggaagctgcg ctcgaccagg ccgttgtacg tgccctcgac gaccacgaag
     1141 ccgacgtcga gggggttgcc gagcgagaag atgcgctcgc cctgcgcgag cgccgcgccg
     1201 gtgtcggggc ggatctggaa cagcccgcgg cgcgcgacgt cggccggcgg cgcggccatg
     1261 cgcacgagcg cgagatcgtg cacgacgtcg aacgcgacca gctggagcgc gcccgacgcg
     1321 ccgtcgacca tctggtagct gagccggtag cgctgcggct cgagcgcggc ctggctgacg
     1381 acgtgccagt tggtgaggat cagcccgccc ggtccgacca gcgaccccga gccgacgctt
     1441 gcctggctgt cctggtcgcg cagcagggtg cgcacctgca gcagccgcgg ccggccgtcg
     1501 tcgtagatgc ggcgcgcggg cgcggacatc gcggcgcggt cggactccgc cttcgcgggc
     1561 ccggaggcag ccgcctgcgg cagcagcgcg gccggcgcgg agg
//
LOCUS       DASVLN010000099         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_327248, whole genome shotgun sequence.
ACCESSION   DASVLN010000099 DASVLN010000000
VERSION     DASVLN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327248"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VF453_02090"
     CDS             complement(<1..238)
                     /locus_tag="VF453_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7686462.1"
                     /translation="MNPTSATANRRPRVTTAAAIAPVPRTEPLRQRVYRQLRDAIEAG
                     SLAPGARLPASREHAGLLAVSRNTVLWAVERLQAE"
     gene            complement(315..911)
                     /locus_tag="VF453_02095"
     CDS             complement(315..911)
                     /locus_tag="VF453_02095"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7686463.1"
                     /translation="MPSPWLTPVRLTAPACTLEPLAPAHHDELVDAARDGELWKLWYT
                     TVPEPAGMRAEIDRRLGLQATGAMLPFTVRDARGRAVGMTTYMRADAANRRVEIGSTW
                     TAASAQRTGLNTAAKRLLLGHAFEALECIAVELRTHFCNHQSRRAIERLGAKLDGVLR
                     SHSLGRDGTLRDTCVYSVTAAEWPTVRVHLDWLIARPR"
     gene            complement(1006..>1846)
                     /locus_tag="VF453_02100"
     CDS             complement(1006..>1846)
                     /locus_tag="VF453_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX7686464.1"
                     /translation="YLPWHRTLGRHWLEYLIEALFLALFVLSAGVVATLREDPLLREA
                     LPSPQARAALAGLAMGLVAVAMIYSPWGRRSGTHLNPAMTLAFWRLRKVGHWDLLFYA
                     IAQFAGGALGIAAVRAWLAPAFAAAGTIPLPHGDVGALALAFALELALAAAAMLVILL
                     TINHAALYRWTGAVVGVMVALVATWQPPLSAFGVNPARTVVISLADGRWDGAALGLLA
                     PMLGMLLAVDAYRLLTGREQIACAKLAHNLDGRCIFRCQHPDQARVLALKQMDRQLRA
                     RRS"
BASE COUNT          265 a          630 c          725 g          226 t
ORIGIN      
        1 cctccgcctg cagccgctcc acggcccaca gcacggtatt gcgcgagacg gccagcagtc
       61 ccgcgtgttc gcgcgacgcg ggcagccgcg cgccgggcgc gagcgagccg gcctcgatcg
      121 cgtcgcgcag ctggcggtag acgcgctggc gcagcggctc cgtccggggg acgggcgcga
      181 tggcggcggc ggtggtgacg cgggggcggc ggttggccgt ggcggaggtc gggttcatgg
      241 cgctcgcacc atcatggcgg cggacgatga tgggcgagga gccattctgc gccgacctga
      301 agggccaatg cgtctcagcg cgggcgcgcg atcaaccagt ccaggtgcac gcggacggtc
      361 ggccattcgg cggcggtgac gctgtagaca caggtgtcgc gcagcgtgcc gtcgcggccg
      421 aggctgtggc tgcgcagcac gccgtcgagc ttggccccga ggcgctcgat ggcgcgccgg
      481 ctctggtggt tgcagaagtg cgtgcgcagc tccaccgcga tgcattccag cgcctcgaac
      541 gcgtgtccga gcagcaggcg cttggcggcg gtgttcagtc cggtgcgctg ggccgaggcc
      601 gcggtccacg tcgagccgat ctcgacgcgc cggttggccg cgtcggcgcg catgtaggtc
      661 gtcatcccga cggcgcggcc gcgggcgtcg cgcacggtga acggcagcat cgcgccggtg
      721 gcctgcaggc cgaggcggcg gtcgatctcg gcgcgcattc cggcaggctc ggggacggtg
      781 gtgtaccaga gcttccagag ctcgccgtcg cgtgcggcat cgacgagctc gtcgtggtgg
      841 gcgggcgcga gcggctcgag cgtgcacgcg ggggcggtca gtcggacggg tgtgagccag
      901 ggtgacggca tgggcattcc tcgtggtcgg gaaagcccat tgtgagaacc gcttccatgc
      961 gccggagagc cattgcaggg cgcccggaag agccattcgc gccggtcagg agcggcgcgc
     1021 gcgcagctgg cgatccatct gcttgagcgc gagcacgcgg gcctggtccg gatgctggca
     1081 gcggaagatg cagcggccgt cgaggttgtg ggcgagcttc gcgcaggcga tctgctcgcg
     1141 tcccgtcagc aggcgatagg cgtcgacggc cagcagcatg cccagcatcg gcgcgagcag
     1201 cccgagcgcg gcgccgtccc agcggccgtc ggccagcgag atgacgacgg tgcgcgccgg
     1261 gttgacgccg aacgcggaca gcggcggctg ccacgtcgcc acgagcgcca ccatcacgcc
     1321 cacgacggcg ccggtccagc ggtacagcgc ggcgtggttg atcgtcagca ggatcacgag
     1381 catcgccgcc gccgccagcg cgagctcgag cgcgaaggcg agcgccagcg cgccgacatc
     1441 gccgtgcggc agcgggatcg tgccggccgc ggcgaatgcc ggcgcgagcc aggcgcgcac
     1501 ggccgcgatg cccagcgcgc cgccggcgaa ctgcgcgatc gcgtagaaca gcaggtccca
     1561 gtggccgacc ttgcgcagcc gccagaacgc cagcgtcatc gccgggttga gatgcgtgcc
     1621 cgagcgtcga ccccagggcg aatagatcat cgccacggcg accaggccca tggcgagccc
     1681 ggccagtgcc gcccgcgcct gcggcgaggg cagggcctcg cgcagcaacg gatcctcgcg
     1741 cagcgtcgcg accacgccgg ccgagagcac gaacagggcg aggaacagcg cctcgatcag
     1801 gtactccagc cagtgccggc ccagcgtccg gtgccagggc aggtag
//
LOCUS       DASVLN010000100         5070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_166657, whole genome shotgun sequence.
ACCESSION   DASVLN010000100 DASVLN010000000
VERSION     DASVLN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166657"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VF453_02105"
     CDS             <1..1039
                     /locus_tag="VF453_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7686465.1"
                     /translation="RAEAEHQRSLARAQAERLETLERLGAIGREITRNLEPSAILAAL
                     DQHVRALLDARSLVIHRVSADGRELVMVYGVEAGAPLPPHRIALDDPRDPVACCARER
                     REVMAGADGSHTPSRTGTTPSVMCAPLSDGDRLLGVIAIGSALPRAYAERELAIFRTL
                     CAYGAIALANADAQATLVRQNEQLERLSVSDALTGLSNRLRLGQVLAQEAARQARSGA
                     PVSVILLDLDHFKAINDQHGHPAGDRVLVAIGELLKAGSRQTDEAGRWGGEEFLVVCR
                     DTELDGARALAEKLRERIASHDFAEAGRRTASFGVAQLAAGEDVAALVARVDAALYRA
                     KHGGRDRVETG"
     gene            complement(1526..2290)
                     /locus_tag="VF453_02110"
     CDS             complement(1526..2290)
                     /locus_tag="VF453_02110"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7686466.1"
                     /translation="MSVENAKIAVDGIRKAFTGARGQALQVVGGISLTVADGEFLAIV
                     GPSGCGKSTLMNMLAGFEQPDAGSVRIDGRPVTGPSPKGIVISQQGSVFPWLTVQQNL
                     MFGLNGTEHAETAQERAALADHYAAMVGLKGFENAYPHELSGGMLKRVELARAFIVKP
                     EILYLDEPFSALDALMNLRMRNELLRILAEERHTVLLITHDVEEAIYMADRIVVLSPR
                     PTTIQATFAVDLPHPRRIASVEAQQLREAILAELGL"
     gene            complement(2280..3062)
                     /locus_tag="VF453_02115"
     CDS             complement(2280..3062)
                     /locus_tag="VF453_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7686467.1"
                     /translation="MSSRISRWDTWPARVLAPMVVIAAIVALWWWVVARDDSAIFPTP
                     WAVVTGAWELVLDGTLFEHIGASLMRVGIGFGLALVVAIPLGLWMGWVTPAYRTLNPL
                     FQMLRPISPIAWIPLAILWFGVGDLSPVFLIFISSVFPMVVQTISGVHTIERRYLRAA
                     ANFGVPRRVLFRQVVIPAVLPEVIVGMRIGIGVAWLVVVAAEMIALHSGLGYLIMDSR
                     NAGNRYDLVVASMVIIGLIGLLLDGLTRLLERLKTVRWRYVR"
     gene            complement(3059..3487)
                     /locus_tag="VF453_02120"
     CDS             complement(3059..3487)
                     /locus_tag="VF453_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686468.1"
                     /translation="MKAARVAFLVPLAAVLAAAAWAGFAPLAVDSRDEPFVIPKGTWA
                     RRMAGNKVEILPPRIDLTVGLNDQLLLRNEDDVPQQFGPVLVMPGQSFRLPFERAAAY
                     SFDCTAHASGQMTIVVAEAPATPWARIRWRWNRLRKKTQA"
     gene            complement(3484..4101)
                     /locus_tag="VF453_02125"
     CDS             complement(3484..4101)
                     /locus_tag="VF453_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7686469.1"
                     /translation="MTRRNLLARLLFALSLAAASACAAAADPNPIGLKAGVFDPPQPA
                     ADFPAGLRATDGGELALSRWRGKVVLLVFGFTHCPAVCPTTLATLAEVRRLLGPDAAA
                     VQVVYVTVDPERDDLAHVRSYLAAFDRSFVGGTGEPGALALLRKRYGVTAQKIPPAGG
                     GADYFMSHSTSIYLIDRQGRLRALMPFGHTAADFAHDVRQLMALR"
     gene            complement(4098..>5070)
                     /locus_tag="VF453_02130"
     CDS             complement(4098..>5070)
                     /locus_tag="VF453_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7686470.1"
                     /translation="AALPGLLPLSAQAAADPLVVGGLAVTCNLTLPVACSARAALNQK
                     SGSSQLAYEYSKFSGWPEVKESLMSGRIQAAYMLAPLVMDLADKRIPVKIVSLGHRSG
                     AVIMVRTDSSYQKFRDLAGKRIAVPSRFAVDFLFLRKMLARENMTPKDIVLVEMAPAD
                     MPAALYAKAVDAYCTGEPFGAAAQRAGYARVLRMTRDEWRNYICCVLTVREELIHQNR
                     PLVQDLVDMVAGAGLWLDQAPAHRDKAVAIAAGRKFFNQDPKILEFVMQNPPDRVTYG
                     DLRMIRSEFDELMQLSVEAGTLKAPVPYEKYVDDSFAKAVKPTAIPL"
BASE COUNT          760 a         1832 c         1810 g          668 t
ORIGIN      
        1 ccgcgccgag gccgagcacc aacgcagcct cgcgcgcgcg caggccgagc ggctcgagac
       61 gctggagcgc ctgggcgcca tcggccgcga gatcacgcgc aacctcgagc cctcggccat
      121 cctcgcggcg ctcgaccagc acgtgcgcgc gctgctggat gcgcgctcgc tcgtcatcca
      181 ccgcgtgtcg gccgacggcc gcgagctcgt gatggtctac ggcgtcgagg ccggcgcgcc
      241 gctgccgccg caccgcatcg cactggacga tccacgcgat cccgtggcgt gctgcgcgcg
      301 cgagcggcgc gaggtgatgg ccggcgccga tgggtcgcat accccttccc gtacgggcac
      361 cacgcccagc gtgatgtgcg caccgctgtc ggacggcgac cgcctgctcg gcgtgatcgc
      421 gatcggctcc gcgctcccgc gcgcctacgc ggaacgcgag ctcgcgatct tccgcacgct
      481 gtgcgcctac ggcgcgatcg cgctcgccaa cgccgacgcc caggcgacgc tcgtacgcca
      541 gaacgagcag ctcgagcggc tgtcggtctc ggacgcgctg accggcctgt cgaaccgcct
      601 gcgcctcggc caggtgctcg cgcaggaggc ggcgcgccag gctcgcagcg gcgcgcccgt
      661 ttcggtgatc ctgctggacc tcgaccactt caaggcgatc aacgaccagc atggccatcc
      721 ggccggcgac cgggtgctgg tggcgatcgg cgagctgctg aaggccggct cgcgccagac
      781 cgacgaagcc ggccgctggg gcggcgagga gttcctggtc gtctgccgcg acaccgagct
      841 cgacggcgcc cgcgcgctcg ccgagaagct gcgcgagcgc atcgcgtcgc acgacttcgc
      901 cgaggccggc cgccgcaccg cgagcttcgg cgtcgcgcaa ctcgcggccg gtgaggacgt
      961 cgcggcgctg gtggcgcgcg tcgacgcggc gctgtaccgc gccaagcacg gcgggcgcga
     1021 ccgggtcgag accggatagt gccggcgagg gcgcgtcgcc gcaggccggc accggggctt
     1081 gaaccacgag cgcggccgcg atgcgccgat gcgccgatgc ccggagcccg gagcccggag
     1141 ctcggagctc cgacgatgcc ttccacggct gcgacggccg gccacgcagc tccggcgact
     1201 tgccgaattg cttcgacgcg tcgtccggga acctcgaagc ccctccctgc gcttgcagcg
     1261 ttgccgccca ggcgatcgac gccggacccg cgagccaggc ggtccgggtg cacgagccgg
     1321 gacgccgatg aaaatcgagg cagtgccggt cttccgcggt cggcgcgccg cactgcgcag
     1381 caggcggccg aggaaattgc cggagccgtc ggagctttct ctcccggcgt cggagcgccc
     1441 ccggggaagc gccgggcctc gcacgacgac gatccgaccg cgcagcccgt cgcgcctggc
     1501 gaccggcgat gtccgccgaa ggccgctaca ggcccagctc cgccaggatc gcctcgcgca
     1561 gctgctgcgc ctcgacgctg gcgatgcggc gggggtgcgg caggtcgacg gcgaaggtgg
     1621 cctggatcgt cgtcggccgc ggcgacagca cgacgatgcg gtcggccatg tagatcgcct
     1681 cctcgacgtc gtgcgtgatc agcagcaccg tgtggcgctc ctcggcgagg atgcgcagca
     1741 gttcgttgcg catgcgcagg ttcatcagcg cgtcgagcgc cgagaacggc tcgtcgaggt
     1801 acaggatctc gggcttgacg atgaaggcgc gcgccagctc cacgcgcttg agcatgccgc
     1861 ccgacagctc gtgcggatac gcgttctcga agcccttgag gcccaccatc gcggcgtagt
     1921 ggtcggccag cgcggcgcgc tcctgcgcgg tctcggcatg ctcggtgccg ttgaggccga
     1981 acatcaggtt ctgctgcacc gtcagccacg ggaacacgga gccctgctgc gagatcacga
     2041 tgcccttcgg gctcgggccc gtcacgggcc ggccgtcgat gcgcacgctg ccggcgtcgg
     2101 gctgctcgaa gcctgccagc atgttcatca gcgtcgactt gccgcagccg gaaggcccga
     2161 cgatggccag gaactcgccg tccgccacgg tcaggctgat gccgccgacg acctgcagcg
     2221 cctggccgcg cgcgccggtg aaggccttgc ggatgccgtc gacggcgatc ttcgcgttct
     2281 caacggacat agcgccaccg caccgtcttc agtcgctcga gcaggcgcgt caggccgtcg
     2341 agcaggaggc cgatcaggcc gatgatcacc atgctggcga cgacgaggtc gtagcggttg
     2401 ccggcgttgc gcgaatccat gatcaggtag ccgaggccgg agtgcagcgc gatcatctcc
     2461 gcggcgacga ccaccagcca cgcgacgccg atgccgatgc gcatgccgac gatcacctcc
     2521 ggcagcacgg ccgggatcac gacctggcgg aacagcacgc gccgcggcac cccgaagttg
     2581 gcggcggcgc gcaggtagcg gcgctcgatg gtgtgcacgc cggagatggt ctgcaccacc
     2641 atcgggaaca ccgacgagat gaagatcagg aacaccggcg acaggtcgcc cacgccgaac
     2701 cacaggatgg ccagcgggat ccaggcgatc ggcgagatcg gccgcagcat ctggaacagc
     2761 gggttgagcg tgcggtacgc gggcgtcacc cagcccatcc acagccccag cgggatggcg
     2821 accaccagcg cgaggccgaa gccgatgccc acgcgcatca gcgacgcgcc gatgtgctcg
     2881 aacagcgtgc cgtcgagcac cagctcccac gcgccggtga cgacggccca cggcgtcggg
     2941 aagatcgcgc tgtcgtcgcg cgccaccacc caccaccaca gcgcgacgat cgcggcgatc
     3001 accaccatcg gcgccagcac ccgggcgggc caggtgtccc atcgtgaaat cctcgaactc
     3061 atgcctgcgt cttcttcctc aaccggttcc agcgccatcg gatccgcgcc cacggcgtgg
     3121 ccggcgcctc ggccacgacg atcgtcatct gcccgctcgc gtgcgcggtg caatcgaagc
     3181 tgtaggcggc cgcgcgctcg aacggcagcc ggaagctctg ccccggcatg accagcaccg
     3241 ggccgaactg ctgcggcacg tcgtcctcgt tgcgcagcag gagctggtcg ttcaggccca
     3301 cggtcaggtc gatgcgcgga ggcaggatct ccaccttgtt gccggccatg cggcgcgccc
     3361 aggtgccctt ggggatcacg aagggctcgt cgcgcgaatc gaccgcgagc ggcgcgaagc
     3421 cggcccaggc cgcggccgcg agcacggcgg ccagcggcac caggaatgcg acgcgcgccg
     3481 ccttcaccgc agcgccatca gttggcggac atcgtgcgcg aagtccgccg ccgtgtggcc
     3541 gaagggcatc agcgcccgca gccggccctg gcggtcgatc aggtagatcg acgtcgagtg
     3601 gctcatgaaa tagtccgcgc cgccgcctgc cggcgggatc ttctgcgccg tcacgccgta
     3661 gcgcttgcgc agcagcgcga gcgcgccggg ctcgccggtg ccgccgacga agctgcggtc
     3721 gaacgcggcc aggtagctgc gcacgtgcgc gaggtcgtcg cgctccggat cgaccgtcac
     3781 gtagaccacc tgcaccgccg ccgcgtcggg cccgagcagc cggcgcacct cggccagcgt
     3841 ggcgagcgtc gtcgggcaca ccgcggggca gtgcgtgaag ccgaacacga ggagcaccac
     3901 cttgccgcgc caccgcgaga gcgccagctc gccgccgtcg gtggcgcgca gcccggccgg
     3961 gaaatcggcc gccggctgcg gcggatcgaa gacgccggcc ttgaggccga tcgggttcgg
     4021 atcggccgcc gccgcgcacg ccgacgcggc cgccagcgac agcgcgaaca gcagtcgggc
     4081 gaggaggttg cggcgcgtca tagcgggatc gccgtgggct tgaccgcctt ggcgaagctg
     4141 tcgtccacgt acttctcgta gggcaccggc gccttgagcg tgccggcctc gacggacaat
     4201 tgcatcagct cgtcgaactc cgagcggatc atgcgcaggt cgccgtaggt gacgcggtcg
     4261 ggcgggttct gcatcacgaa ctcgaggatc ttcggatcct ggttgaagaa cttgcgcccc
     4321 gcggcgatgg ccacggcctt gtcgcggtgg gcgggcgcct ggtcgagcca gaggccggcg
     4381 cccgccacca tgtcgacgag gtcctgcacc agcggccggt tctggtggat cagctcctcg
     4441 cgcaccgtga gcacgcagca gatgtagttg cgccactcgt cgcgcgtcat gcgcagcacg
     4501 cgcgcgtagc cggcgcgctg ggccgcggcg ccgaagggct cgccggtgca gtaggcgtcg
     4561 acggccttcg catagagcgc ggcgggcatg tcggccggcg ccatctcgac gagcacgatg
     4621 tccttggggg tcatgttctc gcgcgcgagc atcttgcgca ggaacaggaa gtccaccgcg
     4681 aaacggctcg gcacggcgat gcgcttgccc gccaggtcgc ggaacttctg gtacgacgaa
     4741 tccgtgcgca ccatgatcac cgcgcccgag cgatggccca gcgacacgat cttgaccggg
     4801 atgcgcttgt ccgcgagatc catcaccagc ggcgccagca tgtaggccgc ctggatgcgg
     4861 ccggacatca gcgattcctt gacctcgggc cagccgctga acttgctgta ctcgtaggcg
     4921 agctggctgg agccgctctt ctggttcagc gcggcgcgcg cgctgcaggc cacgggcagc
     4981 gtgaggttgc aggtcaccgc gaggccgccg acgacgagcg ggtcggccgc cgcctgcgcg
     5041 gacagcggaa gcagccccgg cagcgccgcg
//
LOCUS       DASVLN010000101         5447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_274380, whole genome shotgun sequence.
ACCESSION   DASVLN010000101 DASVLN010000000
VERSION     DASVLN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274380"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..499)
                     /locus_tag="VF453_02135"
     CDS             complement(11..499)
                     /locus_tag="VF453_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686471.1"
                     /translation="MDKTPDSQSAYGVFKPVGHVLASFPTEEDARGAMSDLRQAGFED
                     VQYYSAEDVRVRAERDIQNSGVLAAIGQELNLVKQQRELAEQGHPFVSVRAPEDEQAR
                     RAGAIAARHHADRAQKFGRLIIEELIEPGSGQRQVAESPDRGLDPQTPSGREDASGGR
                     TH"
     gene            complement(568..1317)
                     /locus_tag="VF453_02140"
     CDS             complement(568..1317)
                     /locus_tag="VF453_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7686472.1"
                     /translation="MGWLLIPWRLLRVLWRVLQGIWINATRLPHVDAAGRDAIVQRWA
                     TQLLRDIGVQVRVSGTLHPGPVLLVANHVSWLDIPTLHASAPQTRFVSKSAIAHWPLV
                     GTLARAGGTLFIERERKRDALRVVHEVAGALRERSTVAVFPEGTTGPGDVVLPFHANL
                     LQAAIATATPVQPVVLRYSQPGHRFSRAAQYLGGTTLVGSLVRVIGARGIVVHVDVLP
                     VEPAGEQERRALAATLRARIEQGLAASAARG"
     gene            complement(1326..2636)
                     /locus_tag="VF453_02145"
     CDS             complement(1326..2636)
                     /locus_tag="VF453_02145"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7686473.1"
                     /translation="MKILIRNGRVVDPASGRDEAADVAVAAGRIVAIGNVPPDFAAER
                     TIEADRCVVAPGLVDLSARLGSTAPGSLEAELAAAAAGGVTSVVCPPDTDPALDEPGL
                     VDMLKFRARKISRCRLFPLGALTQGLAGRELTEMIELTGAGCVGFSQAERPMHDALTL
                     SRAMQYAATCGYAVWLRPQEASLAGGVAASGAVATRLGLSGVPVAAETIAIHTIVELM
                     RATGARVHLCRLSSAAGVELVRRARAEGLPLTADVSINSLHLTDVDIGYFNADMRLVP
                     PLRQGKDRDALRAGLADGTISALVSDHTPVGNDEKTLPFGEATPGGSGLELLLSLALK
                     WGEESKLGLAQSLARVTSEPVRVLGEALGSLGQSAGRLVEGGVADVCVFDPAADWQVT
                     PDALVSRGKHTPFDASSTGTRMPGRVRHTLVAGTVAYAADARRS"
     gene            complement(2677..3639)
                     /locus_tag="VF453_02150"
     CDS             complement(2677..3639)
                     /locus_tag="VF453_02150"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX7686474.1"
                     /translation="MLARRNPQLNANGELIHLLSIEGLPRAVLTQILDTAGTFLSVND
                     RDVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLDIARSSTAKGESLLD
                     TIANLSAMHADMFVVRHGESGAPHLIARHCAPHVHVVNAGDGRHAHPTQGLLDMYTIR
                     HYKKSFQDLTVAIVGDIVHSRVARSDIHALTTLGVPEIRAVGPKTLVPGDLREMGVRV
                     CHDMAEGVKDADVIIMLRLQNERMSGAMLPSAGEYFKSYGLTEDKLALARPDAIVMHP
                     GPINRGVEIDSRVADGTHSVILPQVTFGIAVRMAVMSILAGNDA"
     gene            complement(3639..4139)
                     /gene="pyrR"
                     /locus_tag="VF453_02155"
     CDS             complement(3639..4139)
                     /gene="pyrR"
                     /locus_tag="VF453_02155"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEX7686475.1"
                     /translation="MTQLSLDAEALYGELRAGVGRLMKPDTRIVGIWSGGAWLAERLA
                     ADFRTGAVGTLSSTLHRDDFGERGLGAHEGATSLPFEIEGARILLVDDVLYTGRTIRA
                     VVNELFDYGRPASVELAVLVDRGGRELPIAPVFAAATLSLPASQKLSLAREPDGRFTF
                     AVKESH"
     gene            complement(4136..4552)
                     /gene="ruvX"
                     /locus_tag="VF453_02160"
     CDS             complement(4136..4552)
                     /gene="ruvX"
                     /locus_tag="VF453_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7686476.1"
                     /translation="MTTAAGPAGARTQSFLAFDFGVKRVGVATGNSLMRSAQPLRTIA
                     AEGDARFRAIEALIREWAPDALVVGVPFHPDGAEHDNTQRARRFGRQLHGRFRLAVHE
                     VDERYTTVAAEADGAQDADAAAAALILEQFFREQPT"
     gene            complement(4549..5118)
                     /locus_tag="VF453_02165"
     CDS             complement(4549..5118)
                     /locus_tag="VF453_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX7686477.1"
                     /translation="MADAINLTNQFLIAMPGMVDEAFAGSVVYLCEHSDKGALGLMIN
                     KPVDIRLRNLFEKVDLTLASDDLGDQPVFYGGPVQTERGFVLHERDHAASYNSTLSVP
                     GGLEMTTSKDVLEAIAQGVGPRRVLVTLGYSGWATGQLEEEIGRNGWLNVAAEPAIIF
                     DTPVEQRYEKALALLGIDPRMLSQEAGHA"
     gene            5160..>5447
                     /locus_tag="VF453_02170"
     CDS             5160..>5447
                     /locus_tag="VF453_02170"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX7686478.1"
                     /translation="MNKPHRSDRTLASSPSAAAEPSRVPRALVWFRRDLRVDDHPALA
                     RALREADAVWCVFVLDAAILDPLPRRDRRVAFIRDSLAELDARLAALAQAAG"
BASE COUNT          712 a         1969 c         2003 g          763 t
ORIGIN      
        1 gccgggcccc tcagtgcgtc cggccgccgg acgcgtcctc gcgtcccgaa ggcgtctgcg
       61 ggtcgaggcc gcgatcgggc gactcggcca cctggcgctg gcccgagccc ggctcgatca
      121 gctcctcgat gatcaggcgg ccgaacttct gcgcgcggtc ggcgtgatga cgcgccgcga
      181 tcgcgccggc gcggcgggcc tgctcgtcct cgggcgcgcg caccgacacg aacgggtggc
      241 cctgctcggc gagctcgcgc tgctgcttga ccaggttgag ctcctggccg atggcggcga
      301 gcacgccgga gttctggatg tcgcgctcgg cgcgcacgcg cacgtcctcc gcggagtagt
      361 actgcacgtc ctcgaagccg gcctgccgca ggtcggacat cgcgccccgc gcatcctcct
      421 cggtcgggaa cgaggccagc acgtggccga ccggcttgaa cacgccgtag gcgctctgag
      481 agtcgggcgt cttgtccatg atggggctcc tggctgtcga cttgggccct cctcgggcaa
      541 gtcgcggacc ccggccttgg gcgcccgtca gccccgcgcc gccgaggcgg cgagcccctg
      601 ctcgatgcgc gcccgcagcg tcgccgcgag ggcgcgccgt tcctgctcgc ccgcgggctc
      661 gaccggcagc acgtcgacat gcacgacgat gccgcgcgcg ccgatcacgc gcaccaggct
      721 gcccaccagc gtcgtgccgc ccaggtactg cgccgcgcgg ctgaagcggt gccccggctg
      781 cgaatagcgc agcacgaccg gctgcaccgg cgtcgccgtc gcgatggccg cctgcagcag
      841 gttggcgtgg aagggcagga cgacgtcgcc cggcccggtc gtgccctcag ggaacacggc
      901 caccgtcgat cgctcgcgca gcgcgcccgc gacctcgtgc accacccgca gcgcgtcgcg
      961 cttgcgttcg cgctcgatga acagcgtgcc gcccgcgcgc gcgagcgtgc ccaccagcgg
     1021 ccagtgcgcg atcgccgact tcgacacgaa acgcgtctgc ggcgcgctcg cgtgcagcgt
     1081 cgggatgtcg agccacgaga cgtggttggc caccagcagc accggcccgg gatgcagcgt
     1141 gccgctgacc cgcacctgca cgccgatgtc gcgcagcagc tgcgtggccc agcgttgaac
     1201 gatggcgtcg cggccggcgg cgtcgacgtg cggcagccgc gtcgcgttga tccagatgcc
     1261 ctgcagcacg cgccagagca cgcgcagcag gcgccagggg atgagcagcc agcccacgtc
     1321 ggcggtcagg agcgccgcgc gtcggccgcg taggcgaccg tgcccgcgac cagcgtgtgg
     1381 cgcacgcggc ccggcatgcg cgtgcccgtg gagctcgcgt cgaagggcgt gtgcttgccg
     1441 cggctgacca gggcgtcggg cgtgacctgc cagtcggccg cagggtcgaa gacgcagacg
     1501 tcggcgacgc cgccctcgac caggcgaccc gcgctctgcc cgagcgagcc gagcgcctcg
     1561 cccagcacgc gcaccggctc gctcgtcacg cgggcgagcg actgcgcgag gcccagcttc
     1621 gactcctcgc cccacttgag cgcaaggctc agcagcagct ccaggccgct gcccccgggc
     1681 gtcgcctcgc cgaaaggcag cgtcttctcg tcgttgccca ccggcgtgtg gtcggagacc
     1741 agcgccgaga tcgtgccgtc ggccaggccg gcgcgcagcg cgtcgcgatc cttgccctgg
     1801 cgcagcggcg gcacgaggcg catgtcggcg ttgaagtagc cgatgtcgac gtcggtcagg
     1861 tgcagcgagt tgatgctgac gtcggccgtc agcggcaggc cctcggcccg ggcgcggcgc
     1921 acgagctcga cgccggccgc gctcgacagc cggcagaggt gcacgcgggc gccggtggcg
     1981 cgcatcagct cgacgatcgt gtggatcgcg atcgtctcgg ccgcgaccgg cacgccggac
     2041 aggcccagcc gcgtcgccac cgcgccgctc gcggcgacgc cgcccgcgag cgacgcctcc
     2101 tgcggccgca gccacaccgc gtagccgcag gtcgccgcgt actgcatcgc gcgcgacagc
     2161 gtcagggcgt cgtgcatcgg acgctcggcc tgcgagaagc ccacgcagcc cgcaccggtc
     2221 agctcgatca tctcggtgag ctcgcggccc gcgaggccct gcgtcagcgc gccgagcggg
     2281 aacagccggc agcggctgat cttgcgggcg cggaacttga gcatgtccac cagccccggc
     2341 tcgtccagcg ccggatcggt gtcgggcggg cacacgacgc tggtcacccc gccggccgcc
     2401 gcggcggcaa gctccgcctc gagcgagccc ggggcggtgc tcccgagccg cgccgacagg
     2461 tcgaccagcc ccggcgccac cacgcaccgg tcggcctcga tggtgcgctc ggcggcgaag
     2521 tcgggcggca cgttgccgat ggcgacgatg cggcccgcgg cgacggcgac gtccgcggcc
     2581 tcgtcgcggc cgctggcggg gtcgaccacg cggccgttgc gaatcaggat cttcatcggg
     2641 aaaactccgt cctgcggact gcggtgcggg cctggctcat gcgtcgtttc cggcaaggat
     2701 ggacatcacg gccatgcgca cggcaatgcc gaacgtgacc tgcgggagga tgacgctgtg
     2761 cgtgccgtcg gccacgcgcg agtcgatctc gacgccgcgg ttgatcgggc ccgggtgcat
     2821 cacgatcgcg tcgggccggg cgagggcgag cttgtcctcg gtgaggccgt agctcttgaa
     2881 gtactcgccc gcgctgggca gcatcgcgcc ggacatgcgc tcgttctgca gccgcagcat
     2941 gatgatcacg tcggcatcct tcacgccctc ggccatgtcg tggcagacgc gcacgcccat
     3001 ctcgcgcagg tcgcccggca ccagcgtctt ggggccgacg gcacggatct cgggcacgcc
     3061 cagggtcgtg agcgcgtgga tgtcggagcg cgcgacgcgc gaatgcacga tgtcgcccac
     3121 gatcgccacc gtgaggtcct ggaaggactt cttgtagtgg cggatcgtgt acatgtccag
     3181 cagcccctgc gtcgggtgcg cgtggcggcc gtcgccggcg ttgaccacgt gcacgtgcgg
     3241 cgcgcagtgg cgcgcgatca ggtgcggcgc gcccgactcg ccgtggcgga cgacgaacat
     3301 gtccgcgtgc atcgccgaca ggttggcgat ggtgtcgagc aggctctcgc ccttggccgt
     3361 cgacgagcgc gcgatgtcga ggttgatgac gtcggccgac agccgcttgg ccgcgatctc
     3421 gaaggtcgtg cgggtgcgcg tcgagttctc gaagaacagg ttgaacacgc tcttgccgcg
     3481 cagcagcggc accttcttga cgtcgcggtc gttgaccgag aggaaggtgc ccgcggtgtc
     3541 gaggatctgc gtgagaaccg cccgcggcag gccctcgatc gacagcaggt ggatcagctc
     3601 gccgttggcg ttgagctgcg ggttgcgtcg cgcgagcatc agtgggactc cttgacggcg
     3661 aaggtgaagc ggccgtcggg ctcgcgcgcg agcgacagct tctgcgacgc gggcagcgag
     3721 agggtggccg ccgcgaagac gggggcgatc ggcagctcgc ggccgccgcg gtcgacgagc
     3781 accgccagct ccaccgacgc ggggcggccg tagtcgaaca gctcgttgac gacggcgcgg
     3841 atcgtgcggc cggtgtagag cacgtcgtcg accagcagga tgcgcgcgcc ctcgatctcg
     3901 aacggcaggc tggtcgcgcc ctcgtgcgcg cccaggccgc gctcgccgaa gtcgtcgcga
     3961 tgcagcgtgc tggagagcgt gccgaccgcg ccggtgcgga agtcggcggc caggcgctcg
     4021 gcgagccagg cgccgcccga ccagatgccc acgatccggg tgtcgggctt catcagccgg
     4081 ccgacgccgg cgcgcagttc gccgtagagg gcttcggcgt cgagcgagag ttgcgtcatg
     4141 tgggttgctc ccggaagaac tgttcgagga tcagcgcggc ggccgcggcg tccgcgtcct
     4201 gcgcgccgtc ggcctcggcg gcgaccgtgg tgtagcgctc gtcgacctcg tgcacggcga
     4261 gccggaagcg tccgtgcagt tggcgaccga agcggcgggc gcgttgcgta ttgtcgtgtt
     4321 ccgcgccgtc cggatgaaag ggcacgccga cgacgagggc gtcgggggcc cactcgcgga
     4381 tcagcgcctc gatcgcccgg aagcgcgcgt cgccctcggc cgcgatggtg cgcagcggct
     4441 gcgccgatcg catcaggctg ttgccggtcg cgacgccgac gcgcttgacg ccgaaatcga
     4501 aggccaggaa cgactgcgtg cgggcacctg cggggccggc ggccgtcgtc atgcgtggcc
     4561 ggcttcctgg ctgagcatgc gcgggtcgat gcccagcagc gcgagcgcct tctcgtagcg
     4621 ctgctcgacc ggcgtgtcga agatgatcgc cggctccgcc gcgacgttga gccagccgtt
     4681 gcggccgatc tcctcctcga gctgcccggt cgcccagccg ctgtagccga gcgtcaccag
     4741 cacgcggcgc gggccgacgc cctgggcgat cgcctcgagg acgtccttgc tggtggtcat
     4801 ctccaggccg ccgggcaccg acagcgtcga gttgtacgag gcggcgtggt cgcgctcgtg
     4861 caggacgaag ccgcgctcgg tctgcaccgg gccgccgtag aagaccggct ggtcgccgag
     4921 gtcgtcgctc gcgagcgtca ggtcgacctt ctcgaacagg ttgcgcaggc ggatgtcgac
     4981 cggcttgttg atcatcagcc cgagcgcgcc cttgtcgctg tgctcgcaga ggtagaccac
     5041 cgaccccgcg aaggcctcgt cgaccatgcc gggcatggcg atgaggaact ggttggtcag
     5101 gttgatcgcg tcggccattt cgggatttta ggccagcgcc gcgtgacaat ccggcggcga
     5161 tgaacaagcc ccaccgatcc gaccgcaccc ttgcctcctc cccgtccgcc gccgcggagc
     5221 cgtcgcgcgt gccgcgggcg ctcgtctggt tccggcgcga ccttcgcgtc gacgaccacc
     5281 ccgccctcgc gcgcgcgctg cgcgaggccg atgccgtctg gtgcgtgttc gtgctcgacg
     5341 cggcgatcct cgacccgctg ccgcgccgcg accgccgcgt cgccttcatc cgcgacagcc
     5401 tggccgagct cgacgcccgg cttgccgcgc tcgcgcaagc ggcgggc
//
LOCUS       DASVLN010000102         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_274481, whole genome shotgun sequence.
ACCESSION   DASVLN010000102 DASVLN010000000
VERSION     DASVLN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274481"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VF453_02175"
     CDS             <1..850
                     /locus_tag="VF453_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686479.1"
                     /translation="LVLATLVRLLDTTFIRVGNEEYARGNGSYGLTTLRTRHAGVRDA
                     QVRLTFKGKSGVPHEVRLDDRRVAAVVRRCKALPGQELFQYVEADGSVHKVSSSDVND
                     YLHQIAGTRVTAKDFRTWHGSVLALEFTRVACCGEREPGPARAAANKVIAQVAARLRN
                     TVAVCRKSYIHPEVLALGTALGDDEARAALLEAPWARKTAARRSGSLSAAERRLLALL
                     AHAERARKASERALAGRGICLTAEPPRPSSTRKGRHAESLGFHDAQRPRRPTRRDTAA
                     RRAGDG"
     gene            783..1199
                     /locus_tag="VF453_02180"
     CDS             783..1199
                     /locus_tag="VF453_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7686480.1"
                     /translation="MTRNVHVVRPDETLQRAAQVMDELNVGALPVCDDEGLVGIITDR
                     DITVRATAAGLAPEATKVAAVMTEHARCCTGEQLVSEAMAQMADVQIRRLPVVDGARR
                     VVGIVSLGDLATRHSGHVAAALRRISDPSMPDRSAR"
     gene            1218..>1905
                     /locus_tag="VF453_02185"
     CDS             1218..>1905
                     /locus_tag="VF453_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686481.1"
                     /translation="MTPPSAAPAPSRHRMRRGLRNTLLGLLGFVVLVAVALAVCEALE
                     WPFLRHPLERKLAQILERPVTIGEPFGVRLLGSVRAHAGSLVIGPGAAGAPTLVDEHG
                     QPRDFMRTGAAKLSLGYGTLYHQWKGDGGPLHVKLLDVDGLELNLKRDADGRANWQFG
                     APKPAGAASSPQLPNFDRLTVRNGEVRYRDDPLDLSADAHVATREGAAVAGGAPASAA
                     SSNEALPALRV"
BASE COUNT          254 a          740 c          666 g          245 t
ORIGIN      
        1 gctcgtgctc gcgacgctcg tgcggctgct cgacaccacc ttcatccgcg tcggcaacga
       61 ggagtacgcg cgcggcaacg gctcgtacgg cctgaccacg ctgcgcaccc gccacgccgg
      121 agtgcgcgac gcccaggtgc gcctcacctt caagggcaag agcggcgtgc cgcacgaggt
      181 gcgcctggac gaccgccgcg tcgccgccgt cgtgcgccgc tgcaaggccc tgcccggcca
      241 ggagctgttc cagtacgtcg aggccgacgg cagcgtgcac aaggtgagct cgtccgacgt
      301 caacgactac ctccaccaga tcgccggcac gcgcgtcacc gccaaggact tccgcacctg
      361 gcacggcagc gtgctcgcgc tggagttcac gcgcgtcgcc tgctgcggcg agcgcgagcc
      421 ggggcccgcc agggccgccg ccaacaaggt gatcgctcag gtcgcggcgc ggctgcgcaa
      481 caccgtggcc gtctgccgca agtcctacat ccatcccgag gtgctggccc tgggcacggc
      541 gctgggcgac gacgaggcgc gcgccgcgct gctcgaggcg ccgtgggcgc gcaagaccgc
      601 ggcgcgccgc agcggctcgc tcagcgccgc cgagcggcgg ctgctcgccc tgcttgccca
      661 cgccgagcgc gcgcgcaagg cctcggagcg ggccctggct gggcgcggga tttgcctcac
      721 ggccgaacca ccccgtccat cgtccacgcg caagggccgc catgcagaaa gtctcggatt
      781 tcatgacgcg caacgtccac gtcgtccgac ccgacgagac actgcagcgc gccgcgcagg
      841 tgatggatga gctcaacgtc ggcgcgctgc cggtgtgcga cgacgagggc ctggtgggca
      901 tcatcaccga ccgcgacatc accgtgcgcg cgaccgccgc gggcctcgcg ccggaggcca
      961 cgaaggtcgc cgccgtgatg accgagcacg cgcgctgctg caccggcgag cagctggtga
     1021 gcgaggcgat ggcgcagatg gccgatgtcc agatccggcg cctgcccgtg gtcgacggcg
     1081 cgcgccgcgt cgtgggcatc gtctcgctcg gcgacctggc cacgcgtcat tcgggccacg
     1141 tcgccgccgc gctgcgccgc atctcggatc cgtcgatgcc cgatcgctcc gcgcgctgac
     1201 gagaggccct tcgcacgatg acgccgccgt ccgccgcacc cgcgccttcc cgccatcgca
     1261 tgcggcgcgg cctgcgcaac acgctcctcg ggctgctcgg cttcgtcgtg ctcgtcgcgg
     1321 tcgcgctggc cgtctgcgag gccctcgagt ggccgttcct gcgccatccg ctcgaacgca
     1381 agctcgcgca gatcctggag cgccccgtga ccatcggcga gcctttcggc gtgcgcctgc
     1441 tcggctccgt gcgcgcgcat gcaggctcgc tggtcatcgg ccccggcgcg gcgggcgcgc
     1501 cgacgctggt cgacgagcac ggccagccgc gcgacttcat gcgcacgggc gcggcgaagc
     1561 tgtccctcgg atacggcacc ctgtatcacc agtggaaggg cgacggcggc ccgctgcacg
     1621 tgaagctgct ggacgtcgac ggcctggagc tgaacctcaa gcgcgacgcc gacggccgcg
     1681 ccaactggca gttcggcgcc cccaagcccg ccggcgccgc cagctcgccc cagctgccca
     1741 acttcgaccg cctcaccgtg cgcaacggcg aggtgcgcta ccgcgacgat ccgctggacc
     1801 tgagcgccga cgcgcatgtc gcgacgcgcg agggtgcggc ggtcgccggc ggtgcgcccg
     1861 cgtcggccgc ctcgtccaac gaagcgctgc ccgcgctgcg cgtga
//
LOCUS       DASVLN010000103         4922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_488558, whole genome shotgun sequence.
ACCESSION   DASVLN010000103 DASVLN010000000
VERSION     DASVLN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488558"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..599
                     /locus_tag="VF453_02190"
     CDS             177..599
                     /locus_tag="VF453_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEX7686482.1"
                     /translation="MMTKTLMILAETAVITWAMLLAASLIRVHGWTPRGLMAAMGNRD
                     GAQDVQGFAARTDRTARNMLENLVLFTALVLAATAGGVTDPHVELGARVFFWARIAYI
                     PIYMLGIPYVRTIAWAISVAGMGIIFVTLVRTLPPGVL"
     gene            complement(609..1124)
                     /locus_tag="VF453_02195"
     CDS             complement(609..1124)
                     /locus_tag="VF453_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HEX7686483.1"
                     /translation="MQQKSLFEDGRLSRPAAAALAASAVVAPALAAALDKPSRHWSTR
                     IWYGLLRKPSFQPPRAVFPVVWTALDGALALGAYRIARLPATPQRNRALGLFAANVAM
                     IAGWGRLFFGHRRLGAAAVGSAALTATASAYVAAARPIDKPAAAAAVPLVAWVGFATV
                     LGTAIWRMNRR"
     gene            1256..1894
                     /locus_tag="VF453_02200"
     CDS             1256..1894
                     /locus_tag="VF453_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX7686484.1"
                     /translation="MLQNLPDFVGHALRDQRAGLDQLAFNRLPLRAGQGAIKVTSLAF
                     ADHGPLPRACTADGEGHSPPLQWTDVPPAAHGVVLIVEDADSPTPHPLVHAIAVGLAP
                     GDGTLREGALDDEADAPDEARLGRNSYLQHGWLPPDPPPGHGEHRYAFQVFALGAGAA
                     LPETPGRDALMDVLERHAIASGLLIGTYVRHDTRERDDEPKVYTMPAGLAPD"
     gene            1899..2408
                     /locus_tag="VF453_02205"
     CDS             1899..2408
                     /locus_tag="VF453_02205"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX7686485.1"
                     /translation="MAAALRAVRADITTRAVDAIVNAANSSLLGGGGVDGAIHRAAGP
                     QLLQACRALDGCRTGDAKATPGFRLPARHVIHAVGPVWHGGGRGEAELLASCYRRSLE
                     LARELGARSVAFPCIATGVYGYPPDRAADVAVATVRETAARLDAFDEVVFCCFSAGDL
                     ALYEARLAG"
     gene            2877..>4922
                     /gene="fusA"
                     /locus_tag="VF453_02210"
     CDS             2877..>4922
                     /gene="fusA"
                     /locus_tag="VF453_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7686486.1"
                     /translation="MTRTHTPALRDLRNIGVIAHVDAGKTTTTERILFYTGEHHRIGE
                     VHDGDTVMDFDDQERKRGITINSAAVTVHWKGTQVNLIDTPGHIDFNIEVNRSLRVLD
                     GAVVVFDGVAGVEPQTETNWRLADKYRVPRIAFVNKLDRTGADFARVVAMLEERLGVV
                     AVPLQLPIGAEGDFRGVVDLLAMRALVWDRDDASQPARVTEVPPALLDAARRARSRLV
                     ETAVEQDDAALEAWLRGDEIDAGTLRALLRRGTLAGAFVPVLAGSAFRNRGVEPLLDA
                     VVDYLPRPGEVLHGDDAIEADPEAPFAALAFKVVADDHGAMTFVRVYRGRLERGSSVF
                     NATTGKAERASRIYEVHAADRIEKDVLVAGDIAAIVGLKDTLTGHTLADRAHPLQLEA
                     IAVPEPVIDVAIEPRTQADGQHLAKALHAMVREDPSLHVRQDADSGQTLLSGMGELQL
                     EVAVEKLRSRHGVEVAVGRPQVAYRETISLDTEVRYVHKKQSGGPGQFAELVLRLAPL
                     PRGEGLRFANASVGGAIPREFVPAIEAGIRRAAATGVLAGFPVVDFEATVVDGSAHER
                     DSSTLAFELAAAAAFREAAGRAQPLLLEPVMAVEVVTPVEHLGDVIGDLHRRRGHVQA
                     QAPRGNATVVAALVPLKEMFGYIGQLRALTSGRAQYTMQPSHYDAAPARARAALADPA
                     "
BASE COUNT          670 a         1849 c         1767 g          636 t
ORIGIN      
        1 tcctggccct gccggtgccg accatcgagc gcgccggcgg ctccgtgcgg tgcatgctcg
       61 cgggcatcca cctcgcgccg cggtgaggcg ccgcgccggg ccagggtatg cgcgcaccgg
      121 cgccgcgccg cggcgaccgt atgctggcgc cctcctcact gccgggcact gccatcatga
      181 tgaccaagac gctgatgatc ctcgcggaga ccgcggtgat cacctgggcg atgctgctcg
      241 ccgccagcct gatccgcgtc catggctgga cgccccgcgg cctgatggcc gccatgggca
      301 accgcgacgg cgcgcaggat gtgcagggct tcgcggcgcg caccgaccgc actgccagga
      361 acatgctcga gaacctcgtg ctgttcacgg ccctcgtcct cgcggcgacg gccggcggcg
      421 tcaccgaccc gcacgtcgag ctcggcgccc gcgtcttctt ctgggcgcgg atcgcctaca
      481 tcccgatcta catgctcggc atcccctacg tgcgcacgat cgcgtgggcg atcagcgtcg
      541 ccggcatggg catcatcttc gtcacgctcg tgcggacgct gccacccggc gtgctctgac
      601 tttcgcgctc agcgccggtt catgcgccag atcgccgtgc ccagcacggt cgcgaagccg
      661 acccaggcga ccagcggcac ggcggccgcg gcggcgggct tgtcgatcgg ccgggcggcc
      721 gcgacatagg ccgacgccgt ggcggtgagg gccgccgagc cgaccgccgc cgcgcccagg
      781 cggcgatggc cgaagaacag ccgtccccag cccgcgatca tcgcgacgtt ggccgcgaac
      841 aggcccagcg cacggttgcg ctgcggagtg gccggcaagc gggcgatgcg gtaggcgccg
      901 agcgcgagcg cgccgtccag cgccgtccag accaccggaa agaccgcgcg cggcggctgg
      961 aacgacggct tgcgcagcag gccgtaccag atgcgcgtcg accagtggcg cgacggcttg
     1021 tccagcgcgg ccgcgagcgc gggcgcgacg accgcgcttg ccgccagtgc ggcggcggcc
     1081 ggtcgcgaca ggcggccgtc ttcgaacagg gacttctgct gcatcgggtt ctccacggaa
     1141 gcgatcggtc cgctcggcaa ggcgcgcgcc cgaggccacg cacgccgctt gccgcgagcg
     1201 cggatcgggc gccgtcgcgg cgtccgttcc tcgtccctgc cggagggctt tcgccatgct
     1261 ccagaacctg cccgatttcg tcggccacgc gctgcgcgac cagcgcgccg gcctcgacca
     1321 gctcgccttc aaccgcctgc ccctgcgggc cggccagggc gcgatcaagg tcacgagcct
     1381 cgccttcgcc gaccacggcc cgctgccgcg cgcctgcacc gccgacggcg agggccattc
     1441 gcccccgctg cagtggacgg acgtgccgcc cgcggcgcat ggcgtcgtgc tgatcgtcga
     1501 ggacgccgac tcgccgacgc cgcatccgct cgtgcacgcg atcgccgtgg gcctcgcgcc
     1561 cggcgacggc acgctgcgcg aaggcgcgct cgacgacgag gccgacgccc ccgacgaggc
     1621 ccgcctcggt cgcaattcct acctgcaaca cggctggctg ccgccggatc cgccgcccgg
     1681 ccacggcgag catcgctacg ccttccaggt cttcgcgctg ggcgccggcg ccgccctgcc
     1741 cgagacgccg ggccgcgacg cgctgatgga cgtcctcgag cggcatgcca tcgcgagcgg
     1801 cctgttgatc ggcacctacg tgcgccacga cacgcgggag cgcgacgacg agcccaaggt
     1861 ctacacgatg ccggccgggc tggcgccgga ctgacccgat ggccgcggcc ctgcgcgccg
     1921 tgcgcgccga catcacgacg cgcgccgtcg acgcgatcgt caacgccgcg aactcctcgc
     1981 tgctcggcgg cggcggcgtc gacggggcga tccatcgggc ggccggcccg cagctgctgc
     2041 aggcctgccg tgcgctggac ggctgccgca ccggcgacgc caaggccacg cccggcttcc
     2101 ggctgcccgc caggcacgtg atccacgcgg tcgggccggt ctggcacggc ggcggccgcg
     2161 gcgaggcgga actgctcgcc tcgtgctacc ggcgctcgct cgagctcgcg cgcgagctcg
     2221 gcgcgcgcag cgtcgccttc ccctgcatcg ccaccggggt gtacgggtat ccgcccgatc
     2281 gcgcggccga cgtcgcggtg gcgacggtgc gcgagacggc cgcgcgcctg gacgcgttcg
     2341 acgaggtcgt gttctgctgc ttctcggccg gcgacctggc gctctacgaa gcgcggctgg
     2401 ccggctgatc cgcacggacg acgcgaccgc gcgacacgca agaccgcgcg gcttgcagcg
     2461 tcattccgct ttctttatca tgacgaccat catgaaagtc acgctgcatc gccagtcgcc
     2521 gtgccactcg gccgcccgcc aagtgaacaa ggacacgatc gaccactgac gctctccgaa
     2581 catccgagtc ggagggccgc cgcgccttgc cgacatgaac gaaccccggc aggccgcgtc
     2641 ctcccggggt ttcgtttttt ccgcccgcct ccttcgagcc acgccatgcg cacgtacgac
     2701 aagctgatcg ccacgattcc cgatcgccgg agcgcgcacg cacgcccgtg tcccggccac
     2761 ggaagcacga cctccgaccg ctgacgcccc ggtgatcccg ccggggcccg gtcacggacc
     2821 acccggcgcg agagacggcc cgcacgaagc ggccgccgcg cctcgaaagg atcaccatga
     2881 cacgcaccca cacccccgcc ctgcgcgacc tgcgcaacat cggcgtcatc gcccacgtcg
     2941 acgcgggcaa gaccacgacc accgaacgca tcctgttcta cacgggcgag caccaccgca
     3001 tcggcgaagt gcacgacggc gacaccgtca tggacttcga cgaccaggag aggaagcgcg
     3061 gcatcacgat caacagcgcc gcggtcaccg tgcactggaa gggcacgcag gtcaacctga
     3121 tcgacacgcc cgggcacatc gacttcaaca tcgaggtcaa ccgctcgctg cgcgtgctcg
     3181 acggcgcggt ggtcgtgttc gacggcgtgg ccggcgtcga gccgcagacg gagacgaact
     3241 ggcgtctcgc ggacaagtac cgcgtgccgc ggatcgcctt cgtcaacaag ctcgatcgca
     3301 cgggcgcgga cttcgcgcgc gtcgtcgcga tgctcgagga gcgcctgggc gtcgtcgccg
     3361 tgccgctgca gctgccgatc ggcgcggagg gtgacttccg cggcgtcgtc gacctgctcg
     3421 cgatgcgggc gctcgtctgg gaccgcgacg atgcctcgca accggcgcgc gtgaccgagg
     3481 tgccgcccgc gctgctggac gccgcgcggc gggcgcggtc gcgcctggtc gagaccgccg
     3541 tcgaacagga cgacgcggcg ctcgaggcct ggctgcgcgg cgacgagatc gacgcgggca
     3601 cgctgcgcgc gctccttcgc aggggcacgc tggcgggcgc gttcgtgccg gtgctcgcgg
     3661 gctcggcctt ccgcaatcgc ggcgtcgagc cgctgctgga cgcggtcgtg gactacctgc
     3721 cgcggccggg cgaggtcctg cacggcgacg acgcgatcga ggccgatccc gaggccccgt
     3781 tcgcggcgct cgcgttcaag gtcgttgccg acgaccacgg cgcgatgacg ttcgtgcggg
     3841 tctaccgcgg ccggctcgag cgcggctcgt ccgtgttcaa cgcgacgacg ggcaaggccg
     3901 agcgcgcctc gcgcatctac gaggtgcacg cggccgaccg catcgagaag gacgtgctgg
     3961 tcgcgggcga catcgcggcg atcgtcggac tgaaggacac gctcaccggc cacacgctgg
     4021 ccgaccgggc gcatccgctg cagctcgagg cgatcgcggt gcccgagccg gtgatcgacg
     4081 tcgcgatcga gccgcgcacg caggccgacg gccagcacct ggccaaggcg ctgcatgcga
     4141 tggtgcgcga ggacccgagc ctgcacgtgc ggcaggacgc cgattccggg cagacgctgc
     4201 tgtccggcat gggcgagctg cagctggagg tggcggtcga gaagctgcgt tcgcgccacg
     4261 gcgtggaggt ggcggtcggc cggccgcagg tggcctatcg cgagacgatc tcgctggata
     4321 cggaggtaag gtatgtgcac aagaagcaga gcggcggtcc cggccagttc gccgagctcg
     4381 tcctgaggct ggcgccgctg ccgcgcggcg agggcttgcg gttcgccaac gcgagcgtgg
     4441 gcggtgcgat cccgcgcgag ttcgtgccgg cgatcgaggc cggcatccgc cgcgccgcgg
     4501 ccacgggcgt gctggccggc ttcccggtgg tcgacttcga ggccaccgtg gtcgacggca
     4561 gcgcgcacga gcgcgattcg tcgacgctcg ccttcgagct ggctgccgcc gcggcgttcc
     4621 gcgaggccgc cggtcgcgcg cagccgctgc tgctcgagcc ggtgatggcc gtcgaggtgg
     4681 tcacgccggt cgagcacctc ggcgacgtga tcggggatct gcacaggcgc cgcggccacg
     4741 tccaggcgca ggcgccgcgc ggcaacgcga cggtcgtcgc ggcgctggtg ccgctgaagg
     4801 agatgttcgg ctacatcggc cagctgcgcg ccctcacctc gggccgcgcg cagtacacga
     4861 tgcagccgag ccactacgac gcggcgccgg cgcgcgcgcg ggcggcgctg gcggacccgg
     4921 cg
//
LOCUS       DASVLN010000104         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_166848, whole genome shotgun sequence.
ACCESSION   DASVLN010000104 DASVLN010000000
VERSION     DASVLN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166848"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VF453_02215"
     CDS             <1..215
                     /locus_tag="VF453_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686487.1"
                     /translation="WRQVVAALPLVVEADDIAATHARLAAAAVLLKREDDAATHRALA
                     EEALVRHAADQARWTAALEGAGLVLS"
     gene            complement(208..1176)
                     /locus_tag="VF453_02220"
     CDS             complement(208..1176)
                     /locus_tag="VF453_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7686488.1"
                     /translation="MIDITLQNFENDLLQASIQQPVLLDIWAPWCGPCRTLGPLLEKL
                     EADYGGRFVLAKLNADEQQEIAGQLSQAFGVRSIPFCVLFDQGQPVDGFVGAIPEGEI
                     RKFLDRHVPGEDAAAAESEAEAAEALLAEGDTDAALARLQEAVAIDPANEAARFDYIR
                     LLIETSRTRPELLALARQAYEPVAQRVLPNPKLDALGHWLDASEKARAARSHDELAAA
                     IAANKRDFDARFELAQSYFAAGAFTQALDELLEILMRDKSWKDELARKVYVGVLELMS
                     KPAPKPAGAEQPTEKSKLELTGHAAAAPSDPVVDQYRRRLSMTVLS"
     gene            1262..1726
                     /gene="purE"
                     /locus_tag="VF453_02225"
     CDS             1262..1726
                     /gene="purE"
                     /locus_tag="VF453_02225"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7686489.1"
                     /translation="MGSASDWETMKAAAHILSEFAIPFEARVVSAHRMPDDMFAYAEG
                     AVARGLQAIIAGAGGAAHLPGMLAAKTVVPVLGVPVASRHLQGVDSLHSIVQMPKGVP
                     VATFAIGVPGAANAALFAIAMLATRDAELRDRLAAYRARQTEVARAMTQELR"
     gene            1729..2901
                     /locus_tag="VF453_02230"
     CDS             1729..2901
                     /locus_tag="VF453_02230"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX7686490.1"
                     /translation="MPADKQRPLPPGSTLGVLGGGQLGRMFVHAAQVLGYDTLVLDPD
                     PASPAGGAAQRQLCAAYDDARALAEMAGACDAVTTEFENVPAPSLASLAEDVPVAPAA
                     AAVAICQDRAAEKAHFARAGVPCAPHAVIETTAQLAAVADDLLPGILKTSRLGYDGKG
                     QATVADRAALEAAWAALGQVPCVLEKRLPLAYELSIIVARGADGHLVTLPVQQNLHRG
                     GILALTTVPAPDVTQPQSDQAIARASAIAESMDYVGVLCVEFFVLQDGSLVANEMAPR
                     PHNSGHYSIDACDVSQFELQVRTLAGLPLVTPRQHSPCEMVNLLGDLWFDAAGREREP
                     DWPALLALPGMHLHLYGKRQARPGRKMGHLTVTAATPALARERANAAAAVLGLQGF"
     gene            2901..>3195
                     /locus_tag="VF453_02235"
     CDS             2901..>3195
                     /locus_tag="VF453_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEX7686491.1"
                     /translation="MAIVDGMDAQAIARAAEALAAGELVAFPTETVYGLGARADDDAA
                     VAKIFAAKGRPADHPLIVHVADAADAQAFADAWPESARRLADAFWPGPLTVIVP"
BASE COUNT          399 a         1181 c         1175 g          440 t
ORIGIN      
        1 gctggcgcca ggtcgtcgcg gcgctgccgc tggtcgtcga ggccgacgac atcgccgcca
       61 cgcacgcgcg tctggccgcg gcggccgtgc tcctgaagcg cgaggacgac gcggcgacgc
      121 atcgtgcgct cgccgaggaa gcgctcgtgc gccacgccgc cgaccaggcg cgctggacgg
      181 cggcgctcga gggcgccggg ctcgtcctca gctgaggacc gtcatgctca gccggcggcg
      241 gtactggtcg acgaccgggt cgctcggcgc cgcggcggcg tggccggtca gctccagctt
      301 cgacttctcg gtgggctgct cggcgccggc cggcttcggc gccggcttgg acatcagctc
      361 cagcacgccg acgtagacct tgcgcgcgag ctcgtccttc cacgacttgt cgcgcatcag
      421 gatctcgagc agctcgtcga gcgcctgcgt gaacgcgccg gccgcgaagt aggactgcgc
      481 gagctcgaag cgggcgtcga agtcgcgctt gttggcggcg atcgcggcgg cgagctcgtc
      541 gtgcgagcgc gcggcgcgcg ccttctcgct cgcgtccagc cagtgcccga gcgcgtcgag
      601 cttcgggttg ggcagcacgc gctgggcgac cggctcgtag gcctggcggg cgagcgcgag
      661 cagctcgggg cgcgtgcggc tggtctcgat cagcaggcgg atgtagtcga agcgggcggc
      721 ctcgttggca gggtcgatcg cgaccgcctc ctgcaggcgc gcgagcgcgg cgtccgtgtc
      781 gccctcggcg agcagcgcct cggcggcctc ggcttccgat tcggcggcgg cggcgtcctc
      841 gccgggcacg tggcggtcga ggaacttgcg gatctcgccc tcggggatcg cgccgacgaa
      901 gccgtcgacc ggctggccct ggtcgaacag cacgcagaac gggatcgagc gcacgccgaa
      961 cgcctggctc agctggccgg cgatctcctg ctgctcgtcg gcgttgagct tcgcgagcac
     1021 gaagcgcccg ccgtagtcgg cctccagctt ctcgagcagc gggccgagcg tgcggcacgg
     1081 gccgcaccac ggcgcccaga tgtccagcag cacgggctgc tgaatcgacg cctggagcag
     1141 gtcgttctcg aaattctgga gcgtgatgtc gatcattcgg agctcggagg atgacccggc
     1201 taaaatcgtc gttttaccgg gagtgatctt gaacagtgcc tcgcccgtgg tcggcgtcgt
     1261 catggggtcg gcgagcgact gggagacgat gaaggcggcc gcccacattc tctccgagtt
     1321 cgccattccc ttcgaggcgc gtgtcgtgtc ggcacaccgc atgcccgacg acatgttcgc
     1381 ttacgcggaa ggcgccgtcg cgcgcgggct gcaggcgatc atcgccggcg cgggcggcgc
     1441 ggcgcacctg cccgggatgc tcgcggccaa gacggtcgtg ccggtgctgg gcgtgcccgt
     1501 cgccagccgc cacctgcagg gcgtcgactc gctgcactcg atcgtccaga tgcccaaggg
     1561 cgtgcccgtc gcgaccttcg ccatcggcgt ccccggcgca gccaacgcgg ccctgttcgc
     1621 gatcgcgatg ctcgccaccc gcgacgccga gctgcgcgac aggctcgccg cctaccgcgc
     1681 ccgccagacc gaggtggcgc gcgccatgac ccaggagctc cgttgagcat gccggccgac
     1741 aagcagcgcc cgctgccccc gggctcgacg ctgggcgtgc tggggggcgg ccagctgggc
     1801 cgcatgttcg tgcacgccgc gcaggtgctg ggctacgaca cgctggtgct cgaccccgac
     1861 ccggcgagtc ccgccggcgg cgccgcgcag cgccagctgt gcgccgccta cgacgacgcg
     1921 cgcgcgctcg ccgagatggc cggcgcgtgc gacgccgtga ccaccgaatt cgagaacgtc
     1981 cccgcgccgt cgctggcgtc gctggccgag gacgtcccgg tcgcgcccgc cgccgcggcc
     2041 gtggcgatct gccaggaccg cgccgccgag aaggcgcact tcgcccgcgc cggcgtgccc
     2101 tgcgcgccgc acgcggtgat cgagacgacc gcgcagctgg ctgccgtcgc cgacgacctg
     2161 ctgcccggca tcctgaagac ctcgcgcctc ggctacgatg gcaagggcca ggcgacggtc
     2221 gccgaccgcg ccgcgctcga ggccgcgtgg gcggcgctgg gccaggtgcc ctgcgtgctg
     2281 gagaagcgcc ttccgctcgc ctacgagctg agcatcatcg tcgcccgcgg cgccgacggt
     2341 catctggtga cgctgccggt gcaacagaac ctgcaccgcg gcggcatcct cgcgctgacc
     2401 accgtgcccg cgccggacgt gacgcagccg cagtccgacc aggccatcgc gcgcgcgtcg
     2461 gcgatcgcgg agtcgatgga ctacgtcggc gtgctgtgcg tcgagttctt cgtgctgcag
     2521 gacggctcgc tggtcgccaa cgagatggcg ccgcggccgc acaactccgg ccactacagc
     2581 atcgacgcat gcgacgtgtc gcagttcgag ctgcaggtgc gcacgctcgc cgggctgccg
     2641 ctcgtgacgc cgcgccagca ctcgccgtgc gagatggtca acctgctcgg cgacctctgg
     2701 ttcgacgccg ccgggcgcga gcgcgagccg gactggccgg cgctgctggc gctgcccggc
     2761 atgcacctgc acctgtacgg caagcgccag gcgcgccccg gccgcaagat gggccacctc
     2821 accgtcaccg ccgcgacgcc ggcgctcgcg cgcgaacgcg ccaacgccgc ggccgcggtg
     2881 ctggggctgc aagggttctg atggcgatcg tggacgggat ggacgcgcag gcgatcgccc
     2941 gcgccgccga ggcgctggct gcgggcgagc tggtcgcgtt cccgaccgag accgtctacg
     3001 gcctgggcgc gcgcgcggac gacgacgccg cggtcgcgaa gatcttcgcc gccaagggcc
     3061 ggcccgccga ccatccgctg atcgtgcacg tcgccgacgc cgccgatgcg caggccttcg
     3121 cggacgcctg gcccgagtcc gcgcgccggc tcgcggacgc gttctggccc ggaccgctga
     3181 cggtcatcgt gccgc
//
LOCUS       DASVLN010000105         4265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_5474, whole genome shotgun sequence.
ACCESSION   DASVLN010000105 DASVLN010000000
VERSION     DASVLN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5474"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..392
                     /locus_tag="VF453_02240"
     CDS             84..392
                     /locus_tag="VF453_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686492.1"
                     /translation="MRPPHRFIVLACALLAAATPLLGGAFAWRLLDESRPLEAWFQLL
                     YWPSLLLDALPPRAAEGLARSPLPTVILYFLAYLGVGQVAQVVAALVRALLRRPPVRA
                     "
     gene            389..778
                     /locus_tag="VF453_02245"
     CDS             389..778
                     /locus_tag="VF453_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004523770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7686493.1"
                     /translation="MTVPADPVVVVREFWARMASNDFASVGAVLADGFTCDWPQSGER
                     IRGRDHFARMNAEYPAHGRWTFEIHRLFGHGSEIVSDVGVSDGVVRARAISFFTVVDG
                     RIARLVEFWPDPFPAPAQRAHLVEPIA"
     gene            843..1373
                     /gene="yidD"
                     /locus_tag="VF453_02250"
     CDS             843..1373
                     /gene="yidD"
                     /locus_tag="VF453_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX7686494.1"
                     /translation="MPSRLARALLLAAIRAYKRHVSPRKGFACAYRVHTGRASCSTLG
                     YRAVARHGVFRGLGILDARLAQCRLKHERYGPKPAPRSAPRLPPSQAGFVDGDCGGCD
                     GDYCNGGDLVEDVAGCLLPDDCCDDCEPCDRRRRRRKPVRERGRRGSPGASPPVEPSP
                     AADVDATARRFRRAGT"
     gene            complement(1354..1938)
                     /gene="metW"
                     /locus_tag="VF453_02255"
     CDS             complement(1354..1938)
                     /gene="metW"
                     /locus_tag="VF453_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX7686495.1"
                     /translation="MSERSDLAIIEQLVPRGSRVLDLGCGTGEVLAHLQQNHGCTGYG
                     IEIADEGVLASTRRGINVIQLNLEEGLALFDDRSFDVVLQLDTLQHLRNTEKMLRETA
                     RVGRIGIVSFPNFAHWPNRLRIASGRMPVTRALPYEWYDTPNIRVTTYADFEVLARKN
                     GLEILESFGLQHGRVVRAMPNLRASVAVFKFQRA"
     gene            complement(2000..3202)
                     /locus_tag="VF453_02260"
     CDS             complement(2000..3202)
                     /locus_tag="VF453_02260"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX7686496.1"
                     /translation="MPALGASPGSVGRVEPQSLHFAQPLPLASGAQLADYTLVYETYG
                     TLNAARDNAVLVCHALNASHHVAGVSPAGATGWWDNLVGPGKPLDTDRFFVIGVNNPG
                     SCFGSTGPTSPNPDPAAGGQPYGADFPVVTVEDWVDAQARLVDRLGIDKLAAVLGGSL
                     GGMQALAWTLRYPARVGHCVAVATAPNLSAQNIAFNEVARRAIITDPDFHGGHYARHG
                     AIPKRGLRVARMIGHITYLSDDSMEAKFGRATRHAPDDRPYLYTTQDIEFQVESYLRH
                     QGDKFSEYFDANTYLLITRALDYFDPAGAHGGDLSRAFAPARDVRFLVVSFTTDWRFS
                     PQRSREIVKALVDNRTEVSYAEIDAPHGHDAFLLDDPRYHDAMRAYFGRIADGLESQR
                     QRVVKVAV"
     regulatory      complement(3249..3325)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            3483..>4265
                     /locus_tag="VF453_02265"
     CDS             3483..>4265
                     /locus_tag="VF453_02265"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEX7686497.1"
                     /translation="MLLLAPCAQAQDERADASVDATDPANPKDPLPLWEVGLVGIAAR
                     QPAYPGADGDIGRVRVLPYGIYRGSFLRVDGGVSVRALHTPRVEWDVSGAGAFGSPSS
                     RVSARHGMPDLGLLAEIGPALRINLGDFVRPGRDPRATRLELPVRAVFDVSDGMAHKG
                     WTFEPRLAHTAWTGARAALVLSAGVLVGDRRINDVYYGVAPQYATADRPAYEARAGLI
                     ATRLGASLRHRVSPALRMIWFAEAETVRGGANEGSPLVRSRQD"
BASE COUNT          528 a         1526 c         1587 g          624 t
ORIGIN      
        1 acaaggtgca cgcgaaggct tccgacgcgg tgtcctgagc gtccgcgcgc gcggcggcgc
       61 gcgcttggac gagaatgcgg gccatgcgcc cgccccatcg cttcatcgtc ctcgcctgcg
      121 cgctgctcgc ggccgccacg ccgctgctcg gcggcgcgtt cgcgtggcgg ctgctggacg
      181 aatcccgccc gctcgaggcc tggttccagc tgctgtactg gcccagcctg ctcctggacg
      241 cgctgccgcc gcgcgcggcc gaggggctcg cgcgctcgcc gctgccgacg gtgatcctgt
      301 acttcctggc ctacctgggc gtcgggcagg tggcgcaggt cgtcgcggcg ctcgtgcgcg
      361 cgctgctgcg acgcccgccg gtgcgcgcat gacggtgccg gccgacccgg tcgttgtcgt
      421 gcgcgagttc tgggcgcgca tggccagcaa cgacttcgca tccgtggggg cggtgctcgc
      481 cgacggcttc acctgcgact ggccgcagtc cggcgagcgc atccgcggcc gcgaccactt
      541 cgcgcgcatg aacgccgaat accccgcgca cggccgctgg accttcgaga tccaccgcct
      601 cttcggccac ggcagcgaga tcgtgagcga cgttggcgtg agcgacggcg tcgtgcgggc
      661 gcgcgcgatc tcgttcttca ccgtggtcga cggccgcatc gcgcggctgg tggagttctg
      721 gcccgacccg ttcccggcgc ccgcccagcg cgcgcacctc gtcgagccga tcgcgtgacc
      781 gcgaccgatg ccggtggccg gctcgccgag acgctcgcgg tcgactcgcg ggccgggtga
      841 cgatgccgag ccgcctcgcc cgcgcgctgc tgctcgccgc gatccgcgcc tacaagcgcc
      901 acgtctcgcc gcgcaagggc tttgcctgcg cataccgcgt ccacacgggg cgggcgagct
      961 gctcgacgct cggctaccgc gccgtcgcgc gccacggcgt gttccgcggg ctcggcatcc
     1021 tggacgcgcg gctggcgcag tgccggctga aacacgagcg gtacgggccg aagccggcgc
     1081 cccggtcggc gccgcggctg ccaccgtccc aggccgggtt cgtcgacggc gattgcggcg
     1141 gctgcgacgg cgactactgc aatggcggcg acctcgtcga ggatgtcgcc ggctgcctgc
     1201 tgccggacga ctgttgcgac gattgcgagc cttgcgaccg ccgccggcgc cgccgcaagc
     1261 cggtgcgcga gcgcggtcgc cgtggttcgc ccggggcatc gccgcccgtc gaaccctcgc
     1321 cggcggccga cgtcgacgcg accgcgcgac gcttcaggcg cgctggaact tgaagaccgc
     1381 cacgctcgcg cgcaggttgg gcatcgcccg cacgacgcgt ccgtgctgca agccgaagga
     1441 ctcgaggatc tccagcccgt tcttgcgcgc cagcacctcg aagtccgcgt aggtcgtcac
     1501 gcggatgttg ggcgtgtcgt accactcgta gggcagcgcg cgcgtcacgg gcatgcggcc
     1561 cgacgcgatg cgcagccggt tcggccagtg cgcgaagttg gggaagctca cgatgccgat
     1621 gcggccgacg cgcgccgtct cgcgcagcat cttctcggtg ttgcgcaggt gctggagcgt
     1681 gtcgagctgc agcacgacgt cgaagctgcg gtcgtcgaac agcgccagcc cttcctcgag
     1741 gttgagctgg atgacgttga tgccgcgccg cgtgctcgcg agcacgcctt cgtcggcgat
     1801 ctcgatgccg tatcccgtgc agccgtggtt ctgctgcagg tgggcgagca cctcgccggt
     1861 gccgcagccc aggtccagca cgcgcgagcc gcgtggcacg agctgctcga tgatcgcgag
     1921 atcggaacgc tcgctcattg gaagacctcc ctgcttcgcg ccgcggtgcg agcccccgcc
     1981 ggaatggcca ggtgtcggct catacggcca ccttcaccac gcgctggcgc tgcgactcga
     2041 ggccgtcggc gatgcgcccg aagtacgccc gcatcgcgtc gtggtagcgc gggtcgtcga
     2101 gcaggaaagc gtcatggccg tgcggggcat cgatctcggc gtaggagacc tcggtccggt
     2161 tgtcgacgag cgccttgacg atctcgcgcg agcgctgcgg cgagaagcgc cagtcggtgg
     2221 tgaagctcac gaccaggaag cgcacgtcgc gcgccggcgc gaaggcgcgc gagagatcgc
     2281 cgccgtgcgc gcccgcgggg tcgaagtagt cgagcgcgcg cgtgatcagc aggtaggtgt
     2341 tggcgtcgaa gtactcgctg aacttgtcgc cctggtggcg caggtagctc tcgacctgga
     2401 actcgatgtc ctgcgtcgtg tacaggtacg gccgatcgtc cggcgcgtgg cgcgtggcgc
     2461 ggccgaactt ggcttccatc gagtcgtccg acaggtaggt gatgtggccg atcatgcgcg
     2521 ccacgcgcag cccgcgcttg gggatcgcgc cgtggcgggc gtagtggccg ccatggaagt
     2581 cggggtcggt gatgatcgcg cggcgcgcga cctcgttgaa cgcgatgttc tgggccgaca
     2641 ggttgggcgc ggtcgccacc gccacgcagt gcccgacgcg cgccggatag cgcaacgtcc
     2701 atgcgagcgc ctgcatgccg cccaggctgc cgcccagcac ggcggcgagc ttgtcgatgc
     2761 cgagccggtc gacgagccgc gcctgcgcgt cgacccagtc ctccaccgtg acgacgggga
     2821 agtcggcgcc gtagggctgg ccgcccgcgg ccgggtcggg attgggcgag gtcgggccgg
     2881 tcgagccgaa gcacgagccg gggttgttga cgccgatcac gaagaagcgg tcggtgtcga
     2941 gcggcttgcc ggggccgacc aggttgtccc accagccggt cgcgccggcg ggcgagacgc
     3001 cggccacgtg gtggctcgcg ttgagcgcgt ggcacacgag cacggcgttg tcgcgcgccg
     3061 cgttgagcgt gccgtaggtc tcgtagacga gcgtgtagtc ggcgagctgc gcgccgctgg
     3121 cgagcggcag gggctgcgcg aagtgaaggg actgcggctc gacgcggccg accgatcccg
     3181 gggaggcacc cagtgctggc atggattgca ggagatgacg gatcccggcc gcgcgaaacg
     3241 ctggcgggca cccgtccggg gcgccccttt tagcgacatt tgtagagcgc ccgcaagccg
     3301 gtgacaaatc ggcgctgaag ctctcagtat agtggcgcgc ggccggcgct gcgcgcgcgg
     3361 gccgattcgg gcggcgccgt ccgcctggtc cgtcctcgcc ccaaccgctg gagcctgcgc
     3421 ccttgaaacc gatcgccgcg tcctttgccc gccccttcag tgccgccggc ctggcggtgg
     3481 cgatgctcct cctcgcaccg tgcgcgcagg cgcaggacga gcgcgccgac gcctcggtgg
     3541 atgccaccga cccggccaat ccgaaggatc cgctgccgtt gtgggaggtc ggcctcgtcg
     3601 gcatcgcggc gcggcagccg gcctatcccg gagctgacgg cgacatcggg cgcgtgcgcg
     3661 tgctgcccta cggcatctac cgcggctcgt tcctgcgcgt ggacggcggc gtcagcgtgc
     3721 gcgcgctgca cacgccgcgc gtcgagtggg acgtgagcgg cgccggcgcg ttcggctcgc
     3781 cgtccagccg ggtgtccgcg cgccacggca tgcccgacct gggcctgctg gccgagatcg
     3841 gcccggcgct gcggatcaac ctcggcgact tcgtgcgccc cggccgcgac ccgcgtgcca
     3901 cgcgcctgga gctgccggtg cgcgccgtct tcgacgtgag cgacggcatg gcgcacaagg
     3961 gctggacctt cgagccgcgg ctcgcgcaca ccgcctggac gggcgcgcgg gcggcgctgg
     4021 tgctcagcgc gggcgtgctg gtgggcgatc gccgcatcaa cgacgtctac tacggcgtcg
     4081 ccccgcagta cgccaccgcc gatcgcccgg cctacgaggc gcgggcgggc ttgatcgcga
     4141 cccgcctggg cgccagcctg cgccaccgcg tcagcccggc gctgcgcatg atctggttcg
     4201 ccgaggccga gaccgtgcgc ggcggcgcca acgagggcag cccgctcgtg cgcagccgcc
     4261 aggac
//
LOCUS       DASVLN010000106         4348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_327604, whole genome shotgun sequence.
ACCESSION   DASVLN010000106 DASVLN010000000
VERSION     DASVLN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327604"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VF453_02270"
     CDS             complement(<1..209)
                     /locus_tag="VF453_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686498.1"
                     /translation="MTVALPAVALDLWSAAPARGVAVEVLPDGCRDLIVHLPRGGAPA
                     CFVSALAATSETVAIAAGDRLAGVRL"
     gene            complement(241..852)
                     /locus_tag="VF453_02275"
     CDS             complement(241..852)
                     /locus_tag="VF453_02275"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7686499.1"
                     /translation="MPDFPDVPNAPPVDLARALAIQAHANRLANRRLQNALSNLSTSE
                     LHAKRTSFFPSLIGTLNHILAVDLYYIAALHGEADAEQQYSRFAPHDELAPYVSAQER
                     SDQRLIAFCDRLDADAANAIVAMPRGEGRVQRDRAAHVLQHLFMHQTHHRGQVHAMLS
                     GTAVKPPQLDEFLMPSDAQYRVMDMSAVGWTEREVYGTADGLA"
     gene            complement(928..1398)
                     /locus_tag="VF453_02280"
     CDS             complement(928..1398)
                     /locus_tag="VF453_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7686500.1"
                     /translation="MTLPVIDRVARRIAAALQRDGRQSAQQLADSVGLSATPVWRRVK
                     EMEASGVIRGYVALLDREKLGLSICVLAHVTLERHSEGAVEQFETMVRASSEIMECHS
                     TTGEGDYLIKVVAPDMKAYDRFLQDKVFRTPGVANVRSSVVLREVKYETSLPVE"
     gene            1589..>4348
                     /locus_tag="VF453_02285"
     CDS             1589..>4348
                     /locus_tag="VF453_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX7686501.1"
                     /translation="MTLPSSDITATPDPRDDDAAAPLRDYALTDNLAAESGAVFLTGT
                     QALVRLLLMQRRRDQSVGLNTAAFVSGYRGSPLGMVDQQLWKAKKFLKDAQVEFLPAI
                     NEDLAATACLGTQRVALDPKRSVDGVAAMWYGKGPGVDRSGDALKHGNVYGSSAHGGV
                     LVVAGDDHGCVSSSMPHQSDLAMQAWSMPVLHPANVAEYLEFGLYGWALSRFSGAWVG
                     FKAISEVVESGMTVDLDAVPLDFTLPVDFQPSQDLHVRAVDLPSLALESRLAEKLAAV
                     RAFAKVNSVDKHIVSNPNATLGIVTVGKAHFDFLEVLRRLELDPNALAAAGVRIYKVG
                     LVFPLEPTRMAEFAQGLEEILVIEEKAPVVERQLKDLLYGLPDLQRPRIAGKTDAAGR
                     PLLSALGELRPSRIMPVVADWLARLNPALDRRHLVTDFTMPCLLHNEADAVRRLPYFC
                     SGCPHNTSTKVPEGSRALAGIGCHFMASWMERETSGLIQMGAEGVDWAAHSRFTKEKH
                     VFQNLGDGTYYHSGYLAIRQAIAAKATITYKILYNDAVAMTGGQPVDGKLSVPEIARQ
                     VEAEGAKRVVIVSDDIAPHRRHAHLFPQGTTFHPREELDAVQRELREVDGVTILIYDQ
                     TCAAEKRRRRKKGEFPDPPKRVFINEAVCEGCGDCGQASNCLSVIPVETEWGRKRAIE
                     QSSCNKDYSCVNGFCPSFVTLEGAVLRKKSDAGLDKAALAAEIARIGRPARWDWTGPF
                     DMLVTGVGGTGVVTVGALITMAAHLEGKQASVLDFMGFAQKGGAVLSFVRVAPTADQL
                     NQVRIDTQQADVLLACDLVVGASDDALATVKAGRTAILANTHEIATAAFVRNPDATMH
                     APALVDKLRHAAGDGRVRLLDAQGLAQALMGDTMPSNIIMLGAAWQRGLVPVSLRALM
                     RAIELN"
BASE COUNT          641 a         1553 c         1535 g          619 t
ORIGIN      
        1 agccgcacgc ccgccaggcg gtcgccggcg gcgatggcga ccgtctcgga cgtcgcggcc
       61 agcgcgctca cgaagcatgc gggcgcgccg ccgcgcggca ggtggacgat gaggtcgcgg
      121 cagccgtcgg gcaacacctc caccgcgacg ccgcgagccg gcgcggccga ccacaggtcg
      181 agggcgacgg caggcaaggc gacggtcatg ccacggcgcg aagcgcgtct caccggtcgc
      241 tcaggccagc ccgtcggcgg tgccgtagac ctcgcgctcc gtccagccga cggccgacat
      301 gtccatcacg cggtactgcg cgtcgctggg catcaggaac tcgtcgagct gcggcggctt
      361 cacggccgtg cccgagagca tcgcgtgcac ctggccgcgg tgatgcgtct ggtgcatgaa
      421 caggtgctgc aggacgtgcg cggcgcggtc gcgctgcacc cggccctcgc cgcgcggcat
      481 ggcgacgatc gcattggccg cgtcggcatc caggcggtcg cagaacgcga tcaggcgctg
      541 gtcgctgcgc tcctgcgccg acacgtaggg cgcgagctcg tcgtgcggcg cgaagcggct
      601 gtactgctgc tcggcgtcgg cctcgccgtg cagggcggcg atgtagtaca ggtcgaccgc
      661 caggatgtgg ttgagcgtgc cgatcagcga cgggaagaag ctcgtgcgct tcgcgtgcag
      721 ctcggacgtc gagaggttgg acagcgcgtt ctgcaaccgg cggttggcga ggcggttggc
      781 gtgcgcctgg atcgccagcg cgcgcgcgag gtcgaccggc ggcgcgttcg ggacgtcggg
      841 aaagtcgggc atcggatcct ccatgtgtcg tcgtgggcgt cggcgggcgc ggcaatcggc
      901 gcgcgcgcaa gcgggcaagg acttcgctca ctccaccggc agcgaggtct cgtacttgac
      961 ctcgcgcaac acgacgctgc tgcgcacgtt cgcgaccccg ggcgtgcgga agaccttgtc
     1021 ctgcaggaag cgatcgtagg ccttcatgtc cggcgcgacc accttgatca ggtagtcgcc
     1081 ctcgcccgtc gtgctgtggc actccatgat ctcggagctg gcccgcacca tggtctcgaa
     1141 ctgctcgacc gcgccttcgc tgtggcgctc gagcgtgacg tgcgcgagca cgcagatgga
     1201 cagcccgagc ttctcgcggt cgagcagcgc gacgtagccg cggatcacgc cgctcgcctc
     1261 catctccttg acccggcgcc acacgggcgt ggccgagagc ccgaccgagt cggcaagctg
     1321 ctgggcgctc tggcggccgt cgcgctgcag ggcggcggcg atgcggcggg cgacgcggtc
     1381 gatcacgggc agggtcatgg ccccattctg gcgcaatcga aacgtctacc gacgaaaact
     1441 ccaaatcctg agcacggaca tttcgaaagt cccacggatc cgggcatttt cgaacagtcc
     1501 ctgcgccggc ggaggtctac gatttcccgt cccacccgtg cgctgccgcc cgcgggcgcc
     1561 cttccctgtc gagcgagccg aacgcgccat gaccctgccc tcctccgaca tcaccgccac
     1621 gcccgacccg cgcgacgacg acgccgccgc gccgctgcgc gactacgcgc tgaccgacaa
     1681 cctcgccgcc gagtccggcg cggtcttcct cacgggcacc caggcgctgg ttcgcctgct
     1741 gctgatgcag cgccggcgcg accagtcggt cgggctgaac accgcggcgt tcgtctcggg
     1801 ctatcgcggc agtccgctgg gcatggtcga ccagcagctg tggaaggcca agaagttcct
     1861 gaaggacgcg caggtcgagt tcctgcccgc gatcaacgag gacctcgccg ccaccgcctg
     1921 cctgggcacc cagcgcgtcg cgctcgaccc caagcgcagc gtcgacggcg tcgcggccat
     1981 gtggtacggc aagggccccg gcgtcgaccg ctccggcgat gcgctcaagc acggcaacgt
     2041 gtacggctcg agcgcgcacg gcggcgtgct ggtcgtcgcc ggcgacgacc acggctgcgt
     2101 gtcctcgtcg atgccgcacc agagcgacct cgcgatgcag gcctggtcga tgccggtgct
     2161 gcacccggcc aacgtcgccg agtacctcga gttcggcctg tacggctggg cgctgtcgcg
     2221 cttctcgggc gcctgggtcg gcttcaaggc gatctcggag gtggtcgagt cgggcatgac
     2281 ggtcgacctc gacgccgtgc cgctggactt cacgctgccg gtcgacttcc agccgtcgca
     2341 ggacctgcac gtgcgcgccg tcgacctgcc ctcgctcgcg ctcgagtcgc gcctggccga
     2401 gaagctggcc gccgtgcgcg cgttcgcgaa ggtcaactcg gtcgacaagc acatcgtctc
     2461 gaacccgaac gcgacgctgg gcatcgtcac ggtcggcaag gcgcacttcg atttcctcga
     2521 ggtgctgcgc cggctcgagc tcgaccccaa cgcgctggcc gcggcgggcg tgcgcatcta
     2581 caaggtcggc ctcgtgttcc cgctcgagcc gacgcgcatg gccgagttcg cgcagggcct
     2641 ggaggaaatc ctcgtgatcg aggagaaggc gccggtcgtc gagcgccagc tgaaggacct
     2701 gctgtacggc ctgcccgacc tgcagcgccc gcgcatcgcc ggcaagaccg acgcggcggg
     2761 ccggccgctg ctgtcggcgc tgggcgaact gcggccgtcg cgcatcatgc ccgtcgtggc
     2821 cgactggctc gcgcgcctga atcccgcgct cgaccgccgc cacctggtca ccgacttcac
     2881 gatgccctgc ctgctgcaca acgaggccga cgccgtcagg cgcctgccgt acttctgctc
     2941 gggctgcccg cacaacacgt cgacgaaggt gcccgagggc tcgcgcgcgc tcgcgggcat
     3001 cggctgccac ttcatggcga gctggatgga gcgcgagacc agcggcctga tccagatggg
     3061 cgccgagggg gtggactggg ccgcgcactc gcgcttcacg aaggagaagc acgtcttcca
     3121 gaacctgggc gacggcacgt actaccactc gggttatctc gcgatccgcc aggcgatcgc
     3181 cgcgaaggcc acgatcacct acaagatcct ctacaacgac gcggtcgcga tgacgggcgg
     3241 ccagccggtc gacggcaagc tgtcggtacc cgagatcgcg cgccaggtcg aggccgaggg
     3301 cgcgaagcgc gtggtgatcg tgagcgacga catcgcgccg caccgccggc atgcccacct
     3361 gttcccgcag ggcacgacct tccacccgcg cgaggagctc gacgccgtgc agcgcgagct
     3421 gcgcgaggtc gacggcgtga ccatcctgat ctacgaccag acctgcgccg ccgagaagcg
     3481 gcgtcgccgc aagaagggcg agttccccga tccgccgaag cgcgtcttca tcaacgaggc
     3541 ggtgtgcgag ggctgcggcg attgcggcca ggcgtccaac tgcctgagcg tgatcccggt
     3601 cgagaccgag tggggccgca agcgcgcgat cgagcagagc tcctgcaaca aggactactc
     3661 ctgcgtcaac ggcttctgcc cgagcttcgt gacgctggag ggcgcggtgc tgcgcaagaa
     3721 gagcgacgcc ggcctcgaca aggccgcgct cgccgccgag atcgcccgca tcggccgtcc
     3781 cgcacgctgg gactggacgg gcccgttcga catgctggtc acgggcgtgg gcggcacggg
     3841 cgtcgtcacg gtgggcgcgc tcatcacgat ggccgcgcac ctggagggca agcaggcctc
     3901 ggtgctcgac ttcatgggct tcgcgcagaa gggcggcgcg gtgctgtcgt tcgtgcgcgt
     3961 cgcgccgacc gccgaccagc tgaaccaggt gcgcatcgac acgcagcagg ccgacgtgct
     4021 gctcgcctgc gacctcgtgg tcggcgcctc ggacgacgcg ctcgcgacgg tcaaggccgg
     4081 ccgcaccgcc atcctcgcca acacgcacga gatcgccacg gccgccttcg tgcgcaaccc
     4141 cgacgcgacg atgcatgcgc cggcgctcgt cgacaagctg cgccacgcgg ccggcgacgg
     4201 gcgcgtgcgc ctgctcgacg cgcagggcct cgcgcaggcc ctgatgggcg acacgatgcc
     4261 gtccaacatc atcatgctgg gcgcggcgtg gcagcgcggg ctggtgccgg tctcgctgcg
     4321 ggcgctgatg cgggcgatcg agctgaac
//
LOCUS       DASVLN010000107         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_167007, whole genome shotgun sequence.
ACCESSION   DASVLN010000107 DASVLN010000000
VERSION     DASVLN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167007"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VF453_02290"
     CDS             <1..257
                     /locus_tag="VF453_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7686502.1"
                     /translation="WPALVGFGVFGVGIAIVSPCVYAAGARQGAVALAAVMTLGSVGF
                     LVGPLMIGAMAQACGLSWGIALIAAAALLLAACAQRVRWD"
     gene            complement(313..798)
                     /locus_tag="VF453_02295"
     CDS             complement(313..798)
                     /locus_tag="VF453_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7686503.1"
                     /translation="MILSDVFGFTMSPWQIVLRGSVVYWFLFAVFRLVLRRDVGSMGV
                     SDFLFVVLLGDAAQNGMIGDATSTSDAIVLLSTLVFWNVVIDWATFRWPALERVLAST
                     KLKLVVDGRMIRRNMRREFITEEELMSKVRAHGLEDLARVKVMYLEADGEITLVKRDG
                     A"
     gene            complement(930..1514)
                     /locus_tag="VF453_02300"
     CDS             complement(930..1514)
                     /locus_tag="VF453_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX7686504.1"
                     /translation="MEENQTPPVSAESRNSFLEEKAFKSRTVLVFGGINDQTAAETVR
                     RLIALDAESDDPIDMLVSSPGGHLESGEAVHDIIRFINAPVRMIGTGWVGSAATHIYL
                     AVPRERRFCLPHTRFLIHQPSGGAGGQATDIAIQAREIIKARERIAKVIARETGQKIE
                     QVLQDIERDRWLSPDEAIEYGLVSRIIERRTELG"
     gene            complement(1715..2155)
                     /locus_tag="VF453_02305"
     CDS             complement(1715..2155)
                     /locus_tag="VF453_02305"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7686505.1"
                     /translation="MDTITHRYDRPSDAQVRAAEALVSRFAALWRRPDPETFRALMHE
                     DTRNLVPPMTEPADREGVVRHFSEVVRRFPGIRVDVLRWALAGETAMIEWRASATVAG
                     QPLEWTGVDVMRLRGDRMFEAQVYWDTRAVAERAAAAMQATAAG"
     gene            2252..>2557
                     /locus_tag="VF453_02310"
     CDS             2252..>2557
                     /locus_tag="VF453_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7686506.1"
                     /translation="MTTEPRPSRREEYAAATRAALLDAARAQFTEVGFQEAGIEALAR
                     AARVTKGALYHHFPDKRAVFDAVVVALQEDVAARVSRAAGQPARTAERRAEAPLDRLQ
                     "
BASE COUNT          346 a          883 c          951 g          377 t
ORIGIN      
        1 cttggccggc gctggtgggg ttcggcgtct tcggcgtggg catcgccatc gtctcgccgt
       61 gcgtctacgc cgccggcgcg cgccagggcg cggtggcgct ggccgcggtc atgacgctgg
      121 gctcggtcgg cttcctcgtc gggccgttga tgatcggcgc catggcgcag gcctgcgggc
      181 tctcgtgggg aatcgcgctg atcgcggcgg ccgcgctgct gctggcggcc tgcgcgcagc
      241 gggtgcgctg ggactgaagg ccggcgagac cgccggcgtc cgcgtcgacc gcgcgtcggg
      301 acggcgcggc cctcaggccc cgtcgcgctt gaccagcgtg atctcgccgt cggcctccag
      361 gtacatcacc ttcacgcgcg cgaggtcctc caggccgtgc gcacgcacct tggacatcag
      421 ctcctcctcg gtgatgaact cgcgccgcat gttgcgccgg atcatgcggc cgtcgacgac
      481 cagcttcagc ttcgtcgatg cgaggacgcg ctcgagcgcc ggccagcgga aggtggccca
      541 gtcgatcacg acgttccaga acacgagcgt cgacagcagc acgatcgcgt cgctcgtcga
      601 ggtggcgtcg ccgatcatcc cgttctgcgc cgcgtcgccc agcagcacga cgaaaaggaa
      661 gtccgagacg cccatcgagc cgacgtcgcg ccgcagcacg aggcggaaga cggcgaacag
      721 gaaccagtag acgacgctcc cgcgcaggac gatctgccag ggcgacatcg tgaagccgaa
      781 cacgtcggac aggatcatgg cgcctccgtc cgggccgcat gcgggcccgc ggctgcgcca
      841 cggcaaatcc ggcgccaacg caaagggcct tcgcgcccgg gagcggaagg ccctggtccg
      901 gccggccctt tcgaggcgcc tcgcaggcgt cagccgagct cggtgcggcg ctcgatgatc
      961 ctcgagacca ggccgtactc gatcgcctcg tcgggcgaga gccagcggtc gcgctcgatg
     1021 tcctggagca cctgctcgat cttctggccg gtctcgcggg cgatgacctt ggcgatgcgc
     1081 tcgcgcgcct tgatgatctc gcgtgcctgg atcgcgatgt cggtggcctg gccgccggcg
     1141 ccgccgctcg gctggtggat caggaagcgg gtgtgcggca ggcagaagcg gcgctcgcgc
     1201 ggcaccgcca ggtagatgtg cgtggccgcg ctgccgaccc agccggtgcc gatcatgcgc
     1261 accggcgcgt tgatgaagcg gatgatgtcg tgcacggcct cgcccgattc gaggtggccg
     1321 ccgggcgagg acacgagcat gtcgatcggg tcgtccgact cggcgtcgag cgcgatcagg
     1381 cggcgcacgg tctcggcggc ggtctggtcg ttgatgccgc cgaagacgag cacggtgcgg
     1441 gacttgaagg ccttttcctc gaggaacgag ttgcgcgact cggccgagac ggggggtgtt
     1501 tggttctctt ccacgaaagt ctccttggaa cgggatgcgg gaggggcgat ggcgcgatca
     1561 tcccacggcg gccgcagccg cggcgcgggc ccaccgcagc ggtggcacgg gcttgtcgcg
     1621 ggccgcgcgg acgcgaccgc agcggcgata cgtggggacg atcgcccggg acggcaaggg
     1681 ccgcgtcccg cgtcttcccc tccatggccg gcgctcagcc cgccgcggtg gcctgcatcg
     1741 cggcggcggc gcgctcggcg accgcgcgcg tgtcccagta gacctgggcc tcgaacatcc
     1801 ggtcgccccg caggcgcatg acgtcgacgc ccgtccattc gaggggctgg ccggccacgg
     1861 tcgcgctcgc acgccattcg atcatcgccg tctctcccgc cagcgcccag cgcagcacgt
     1921 cgacgcggat gccggggaac cggcgcacga cctcgctgaa gtgccgcacc acgccctcgc
     1981 ggtcggcggg ctcggtcatc ggcggcacga ggttgcgcgt gtcctcgtgc atcagcgcgc
     2041 gaaaggtctc gggatcgggg cggcgccaga gcgccgcgaa gcgcgagacg agcgcctcgg
     2101 cagcgcgtac ctgggcgtcg ctcggacgat cgtagcggtg ggtgatcgtg tccatcggtt
     2161 ctccttgaca gggggacggc gcgcgaaatg cgttccgttg gtatcaaaca tactgtcggt
     2221 atgtgtttga gtcaacggta tccttgcacg catgacgacc gagcccaggc catcgcggcg
     2281 ggaggagtac gctgccgcca cccgcgcggc gctgctggac gcggctcgcg cgcagttcac
     2341 cgaggtcggc ttccaggagg cgggcatcga ggcgctggcc cgcgcggcgc gcgtgaccaa
     2401 gggcgcgctg tatcaccact ttccggacaa gcgggcggtc ttcgacgcgg tggtcgtcgc
     2461 gctgcaggag gacgtggcgg cgcgtgtctc gcgggccgcc ggccagcctg cgcgaaccgc
     2521 ggagcgccgc gccgaggcgc cgctcgaccg cctgcag
//
LOCUS       DASVLN010000108         2751 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_58599, whole genome shotgun sequence.
ACCESSION   DASVLN010000108 DASVLN010000000
VERSION     DASVLN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58599"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1159)
                     /locus_tag="VF453_02315"
     CDS             complement(101..1159)
                     /locus_tag="VF453_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7686507.1"
                     /translation="MTALFAPLPDGPLDIVGDVHGELDALDALLGHLGYDAAGRHPEG
                     RTLVFVGDLCDRGPDSPGVFDRVRALVEDGRAAATLGNHELNLLRGDRKDGNDWFFSE
                     YGARDAKYEPRIHLPPERRGEVIAFMRSLPLLLERPDLRVVHAAWDAASVALLRAADP
                     ALSRVEHFRRWEADADALIAAQGLAELARREYDEYRERLVDPSRPVPMLQATAAYDEI
                     RQMANPIRVVTSGIERRAARPFFASGHWRFVERIRWWDEYADGVPVVVGHYWRQFALV
                     DRASLGKAEENLFTDLSPTAWHGRDGNVFCVDFSVGGRFLERPLAPGGLPRSRLGALR
                     WPEQVLVLDTGESLPTTR"
     gene            1511..1837
                     /locus_tag="VF453_02320"
     CDS             1511..1837
                     /locus_tag="VF453_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686508.1"
                     /translation="MTRMPAPRIHRLAASVAAVSLVALFASSALAATTGYDKRLAALV
                     QTTKEDPSYKRLPLDTAEDKQWFNDATEKLYARQMTKEEYVAEGAKRFPGYEPSLNTV
                     ADFMTK"
     gene            2064..2207
                     /locus_tag="VF453_02325"
     CDS             2064..2207
                     /locus_tag="VF453_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686509.1"
                     /translation="MSKEHRSTKNVKKPKKDAPQPKAGGSDRPVAPMTSVAPKGKLKN
                     KPA"
     gene            2260..>2751
                     /locus_tag="VF453_02330"
     CDS             2260..>2751
                     /locus_tag="VF453_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7686510.1"
                     /translation="MDDLRELTTRFPHAGRLDAILLRPGRGVPAVRVDEALAIAGRGL
                     DGDRSADPGRAGGGKRQVTLMQAEHLPLIAAWTRHAAIDPAVLRRNLVVSGANLLAQR
                     SPFADRPLQVRIGVQVVLEISGPCDPCSLMERALGPGAYNAMRGHGGTTARVLRGGRI
                     AVGD"
BASE COUNT          414 a         1007 c         1007 g          323 t
ORIGIN      
        1 gggatcgccg gcgccggcgc cgtcgcggtc gggagggaaa tcgtgctcgc ggctcatggc
       61 gcgctttcta gcacaggcgc ccagcgcgac cgcggcgccg tcaccgcgtc gtcggcagcg
      121 actcgccggt gtcgagcacc agcacctgct ccggccagcg caacgcgccc aggcgcgagc
      181 gcgggaggcc gccgggcgcg agcgggcgct cgaggaagcg gccgccgacc gagaagtcga
      241 cgcagaacac gttgccgtcg cgaccgtgcc aagcggtggg agagaggtcg gtgaacaggt
      301 tttcctcggc cttgccgagc gaggcgcggt cgacgagcgc gaactggcgc cagtagtggc
      361 cgacgacgac cggcacgccg tcggcgtact cgtcccacca acggatgcgt tcgacgaagc
      421 gccagtggcc gctcgcgaag aacgggcgcg ccgcgcggcg ctcgatgccc gaggtgacga
      481 cgcggatcgg gttggccatc tggcggatct cgtcgtaggc cgccgtcgcc tgcagcatcg
      541 gcaccggccg gctcgggtcg acgaggcgct cgcggtactc gtcgtactcg cggcgtgcga
      601 gctcggccag gccctgcgcg gcgatcagcg cgtcggcgtc ggcctcccag cggcggaagt
      661 gctcgacccg gctgagcgcg ggatcggcgg cgcgcagcag cgcgacggag gccgcgtccc
      721 aggcggcatg gaccacgcgc aggtcgggcc gctccagcag cagcggcagc gagcgcatga
      781 aggcgatcac ctcgccgcgg cgctccggcg gcaggtggat gcgcggctcg tacttggcgt
      841 cgcgggcgcc gtactcgctg aagaaccagt cgttgccgtc cttgcggtcg ccacgcagca
      901 ggttgagctc gtggttgccg agcgtcgcgg ccgcgcgccc gtcctcgacg agcgcgcgca
      961 cgcggtcgaa cacgccgggg ctgtcgggcc cgcggtcgca caggtcgccg acgaagacga
     1021 gcgtgcgccc ctcgggatgg cggccggcgg cgtcgtagcc gaggtggccg agcagcgcgt
     1081 ccagcgcatc gagctcgcca tggacgtcgc ccacgatgtc gagcgggccg tcgggcaggg
     1141 gagcgaagag ggcggtcatc gggcgggagg cggagtccgc gagcatacgt gcaatgcgcg
     1201 cgcccggccg gtccgggggc atgggggcga ggacgacgcg cacaggcgcg gcgtggcagg
     1261 cgaatgaccg catcgcgcgt gcgaccggtc ttgacagcgc gtgcgattcg cgccgtcgcg
     1321 gtcgcgtgcg acgaacggcg aaatgagagg aacgaactgc aacccgctgc gggctcgccg
     1381 cgcctacgat acctccacac gtcaaccgcc cggccggcct gaggtgccgc agcgcaaaca
     1441 tcctgcacgc accggttgag atgcaagaga cggctcacgc cgtcgcctca aacgaaactg
     1501 gagaatcccg atgacccgta tgcctgcccc ccgtatccat cgcttggccg ccagcgtcgc
     1561 cgcggtctcg ctcgtcgccc tgttcgcgtc cagcgccctc gccgccacca ccggctacga
     1621 caagcgcctg gccgccctgg tccagaccac gaaggaagac ccgagctaca agcgcctgcc
     1681 cctcgacacc gccgaggaca agcagtggtt caacgacgcc accgagaagc tgtacgcgcg
     1741 ccagatgacc aaggaggaat acgtcgccga aggcgccaag cgcttccccg gctacgagcc
     1801 ctcgctgaac accgtcgccg acttcatgac gaagtgatcg ggcccgcctg atcgatccga
     1861 catgcgccgg agcgcttccg gcgcccgcgc tccccgtgcc tcgtgcacgc ggggcgcgct
     1921 cgcgtttgcg ctgcgcacgg cctaacgcct cgatgcgtcg cggcgaggtc ccggcccggt
     1981 cccggctggc gaggcgcgtc catgccgtac catgtcgcgc gccgtccacc gaggacgcgg
     2041 aaccgtcgaa ccaggaggtc gccatgagca aggaacatcg cagcaccaag aacgtcaaga
     2101 agcccaagaa ggatgcgccg cagccgaagg ccgggggctc cgaccgaccg gtcgcgccga
     2161 tgacctccgt cgcgcccaag ggcaagctga agaacaagcc ggcctgagcg ccgggcgcgc
     2221 gtgcgcgcgg cgcggcaccg cgatcccggc gtcgatgcca tggacgacct gcgcgagctc
     2281 acgacccgct tcccgcacgc cgggcggctc gacgcgatcc tgctgcggcc cggccgcggc
     2341 gtgcctgcgg tgcgcgtcga cgaggcgctc gcgatcgcgg gacgcggcct cgacggcgac
     2401 cgcagcgccg accccggccg cgccggcggc ggcaagcgcc aggtcacgct gatgcaggcg
     2461 gagcacctcc cgctgatcgc cgcctggacg cgccacgccg cgatcgaccc ggccgtcctg
     2521 cggcgcaacc tcgtcgtctc gggcgcgaac ctgctcgccc agcgctcgcc cttcgccgat
     2581 cgaccgctgc aggtgcgcat cggcgtgcag gtcgtgctcg agatcagcgg cccctgcgac
     2641 ccgtgctcgc tgatggagcg cgcgctcggc ccgggcgcct acaacgcgat gcgcggccac
     2701 ggcgggacga ccgcgcgcgt gctgcgcggc gggcggatcg cggtgggcga c
//
LOCUS       DASVLN010000109         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_167126, whole genome shotgun sequence.
ACCESSION   DASVLN010000109 DASVLN010000000
VERSION     DASVLN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167126"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..998)
                     /gene="hutG"
                     /locus_tag="VF453_02335"
     CDS             complement(183..998)
                     /gene="hutG"
                     /locus_tag="VF453_02335"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HEX7686511.1"
                     /translation="MTDHPAFHLTRGTTPLLVSLPHSGELIPDDLRDAYVPRALALED
                     TDWHLQRLYAFAEGLGASVLRPRASRYVIDLNRPPDDAPMYPGANNTELCPTRFFTGD
                     PLYREGRAPDAAEIARRTTQWWLPYHRALMAELERLRAAHGHAVLFDGHSIRSELPWL
                     FEGRLPALNLGTVNGASCAASLRAALAAMLALQRRHSHVVDGRFKGGYITRHYGRPEE
                     GLHAVQLEMTWACYMDEAPPYAYREERADEVLPTLRELLTIMRDWKPASEGLQ"
     gene            complement(1050..>2180)
                     /gene="hutI"
                     /locus_tag="VF453_02340"
     CDS             complement(1050..>2180)
                     /gene="hutI"
                     /locus_tag="VF453_02340"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX7686512.1"
                     /translation="DGERIAWVGPQSRLPALPARPGRREEEHDLGGLLVTPGLVDAHT
                     HLVYAGNRAHEFELRLQGASYEEIARAGGGIRSTVAATRAADEDTLFELAAQRVRLLR
                     ADGVTTVEVKSGYGLSYEDEARCLRVARRLAGLGVGVRTTSLAAHALPPEFEGRAEAY
                     VDAICSDWLPRWHAEGLVDAVDAFCERIAFTPAQVERVFACAADLGLPVKLHAEQLSD
                     SGGAELACRYGALSCDHLEHLSDAGVAAMRAAGAVALLLPGAFHFLRETKLPPVDALR
                     AAGVPMAVATDHNPGTSPTLSLPLMIHLASVDFRLTPEEAVRGATVNAARALGLADRG
                     TLGVGQRADLALWEHEHPRELAYRFGHAACRRVVIGADDLVS"
BASE COUNT          270 a          783 c          854 g          273 t
ORIGIN      
        1 ccgcgccgac ctccagcagc acggcctcgc gccagccgcc gtcgacccag gccgcgggcg
       61 cccagtaggc gcgcgtggcc gtcatgcgcg agcctccgcc tgccgagccg tgtcgcgcat
      121 cggagcagcc tgcggcactg cgcggtgcgg tgcgaggccc ttcggaccgg ccgggcacgc
      181 gttcattgca gcccctcgct cgcgggcttc cagtcgcgca tgatcgtcag cagctcgcgc
      241 agggtcggga gcacctcgtc ggcgcgctcc tcgcggtagg cgtagggcgg cgcctcgtcc
      301 atgtagcagg cccaggtcat ctcgagctgc accgcgtgca gtccttcttc gggacggccg
      361 tagtggcgcg tgatgtagcc gcccttgaag cggccgtcga cgacgtggct gtggcggcgc
      421 tgcagcgcca gcatggcggc cagcgcggcc cgcagcgacg ccgcgcagct cgcgccgttg
      481 acggtgccga ggttcagcgc gggcagcctg ccctcgaaca gccagggcag ctcgctgcgg
      541 atgctgtggc cgtcgaacag caccgcgtgg ccgtgcgcgg cgcgcaggcg ctcgagctcg
      601 gccatcagcg cgcggtggta cggcagccac cactgcgtcg tgcggcgcgc gatctcggcg
      661 gcatcgggcg cgcggccctc gcggtacagc ggatcgccgg tgaagaagcg cgtcgggcac
      721 agctcggtgt tgttcgcgcc ggggtacatc ggcgcgtcgt cgggcgggcg gttgaggtcg
      781 atcacgtagc gcgacgcgcg cggccgcagc acgctcgcgc caagcccctc ggcgaaggcg
      841 tagaggcgct gcaggtgcca gtcggtatcc tcgagcgcga gcgcgcgcgg cacgtaggcg
      901 tcgcgcaggt cgtcggggat cagctcgccg ctgtgcggca ggctcaccag cagcggcgtc
      961 gtgccgcggg tcaggtggaa ggcgggatgg tcggtcatga ggagctgcaa cctccgttca
     1021 cgcgggcggt cggttcgagg gcttgcggat cacgacacga gatcgtcggc gccgatcacg
     1081 acgcgccggc aggctgcgtg cccgaagcgg tacgcgagct cgcgcgggtg ctcgtgctcc
     1141 cacagcgcga ggtcggcgcg ctggcccacg cccagggtgc cgcggtcggc cagccccagc
     1201 gcgcgcgccg cgttgaccgt cgcgccgcgc acggcctcct cgggcgtgag ccggaagtcg
     1261 acgctggcca ggtggatcat cagcggcagc gacagcgtcg gcgaggtgcc ggggttgtgg
     1321 tcggtcgcga ccgccatcgg cacgcccgcg gcgcgcagcg cgtcgaccgg cggcagcttc
     1381 gtctcgcgca ggaagtggaa ggcgccgggc agcagcagcg cgaccgcgcc ggccgcgcgc
     1441 atcgccgcga cgccggcgtc ggagaggtgc tcgaggtggt cgcacgacag cgcgccgtag
     1501 cggcacgcga gctcggcgcc gcccgagtcg ctcagctgct cggcgtgcag cttcaccggc
     1561 aggcccaggt ccgcggcgca ggcgaagacg cgttcgacct gcgcgggcgt gaaggcgatg
     1621 cgctcgcaga aggcgtcgac cgcgtcgacg aggccctcgg cgtgccagcg cggcagccag
     1681 tccgagcaga tggcgtcgac gtaggcctcg gcgcggccct cgaactcggg cggcagcgcg
     1741 tgcgcggcca ggctggtggt gcgcacgccg acgcccaggc ccgcgagccg gcgcgcgacg
     1801 cgcaggcagc gcgcctcgtc ctcgtacgac aggccgtagc cggacttgac ctcgacggtg
     1861 gtcacgccgt cggcgcgcag caggcgcacg cgctgggcgg cgagctcgaa cagcgtgtcc
     1921 tcgtcggccg cgcgggtggc cgcgacggtc gagcggatgc cgccgccggc gcgcgcgatc
     1981 tcctcgtagc tcgcgccctg caggcgcagc tcgaactcgt gcgcacggtt gcccgcatac
     2041 accaggtggg tatgcgcgtc gacgagcccc ggcgtgacga gcaggccgcc gaggtcgtgc
     2101 tcctcctcgc gccgtccggg acgggcgggc agggcgggca gcctggactg cgggccgacc
     2161 caggcgatgc gctcgccgtc
//
LOCUS       DASVLN010000110         5096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_381314, whole genome shotgun sequence.
ACCESSION   DASVLN010000110 DASVLN010000000
VERSION     DASVLN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381314"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..980
                     /gene="ahpC"
                     /locus_tag="VF453_02345"
     CDS             417..980
                     /gene="ahpC"
                     /locus_tag="VF453_02345"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017114658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HEX7686513.1"
                     /translation="MSLINTQVQPFKTEAFHNGKFVTVSDETLKGKWSVLIFMPAAFT
                     FNCPTEVEDAADNYAEFQKLGAEVYIVTTDTHFSHKVWHETSPAVGKAKFPLVGDPTH
                     TLTNAFGVHIPEEGLALRGTFIINPEGVIKTAEIHSNEIARDVKETLRKLKAAQYTAA
                     HPGEVCPAKWNEGAKTIAPSLDLVGKI"
     gene            1017..2585
                     /gene="ahpF"
                     /locus_tag="VF453_02350"
     CDS             1017..2585
                     /gene="ahpF"
                     /locus_tag="VF453_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEX7686514.1"
                     /translation="MLDSNLKAQLKAYLERVKLPFVITASLDEGPSSQEMHEFLQDIV
                     GLTDKITLKTDGTAARRPSFSLARAGEAARIEFAAIPMGHEFTSLVLALLQVGGHPPK
                     IEQELIDQIKGLEGDLRFETYMSLTCHNCPDVVQALNLLAVLNPRIQSVVIDGGLYQQ
                     EVAERDVLAVPMVFLNGQHFGQGRMDVEEIVKKLDTGSAARDAAKLNQKDAYDVLIVG
                     GGPAGAAAAVYAARKGIRTGLLAERMGGQTMDTMSIENFISVLETDGPKFAAALEQHA
                     KRYEVDILNLQRAQKLEPAGADGLLGVELANGAKLRSKTVIVATGARWREVNVPGEKE
                     FRNKGVAYCPHCDGPLFKGKSVAVIGGGNSGVEAAIDLAGIVAHVDLLEFGEQLRADA
                     VLVNKLGSLPNVAIHTRAQTTSIDGTDGKVSGLTYTDRATGESRKLALEGVFVQIGLV
                     PNSEWLKGTLELTKHGEIVTDARGQTSLPGVFAAGDVTTVPFKQIIIATGDGAKAALG
                     AFDHLIRSSAPVAA"
     gene            complement(2677..3582)
                     /gene="rarD"
                     /locus_tag="VF453_02355"
     CDS             complement(2677..3582)
                     /gene="rarD"
                     /locus_tag="VF453_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX7686515.1"
                     /translation="MSPGLAYAASAYFLWGLFPLYFHALEQVDAFELVVHRSLWSFAF
                     IWAVLAATRRWQWLGAVARSPRLWGQFLLSALLLSGNWLLYIWSVNNGHVIEASLGYF
                     VTPLVNVLLGTAVLRERPRRLQWAAVGIAALGVAWLTVTIGRPPWIAFGLALSFGCYG
                     LLRKTAPLGALEGLAMETLVLAPVAIAAMAVLGARHGGVFTGFSAGTVAMLLAAGPVT
                     AIPLLLFAAGARRVTMVTLGTLQYLSPSLQFGLGVWWFGEPLDAHRLAGFVLIWASLA
                     VYSLDGFLWMRRQQAVPAVARPARG"
     gene            3687..4220
                     /locus_tag="VF453_02360"
     CDS             3687..4220
                     /locus_tag="VF453_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686516.1"
                     /translation="MPQTFNVGSRSRFVNLVAWLAILLGAFACAWAAIQNAAHSSWAP
                     AAAARDGLPWLSGLLLRYLPWVFSSAVALSLATLVCAVGLLQRVEWARRLFIGLLAVA
                     IVVVLGGVWLQQELAHLVVDDTLRHAALSQRAAELFGGMVIVTRALAGALSVTAGLAL
                     AAVMRRLMSPAVRQEFA"
     gene            complement(4225..4620)
                     /locus_tag="VF453_02365"
     CDS             complement(4225..4620)
                     /locus_tag="VF453_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEX7686517.1"
                     /translation="MPIASDSPCPCGRPLAYAACCGRWHAGPDALRAPTAEDLMRSRY
                     SAFAAGLLDYLRETWHPSTRPAELEPLPAGLRWLGLEVRRHVRQDDDHAIVEFVARSK
                     LGGRAQRLHETSRFVREGGRWFYVSGDVG"
     gene            4834..>5096
                     /locus_tag="VF453_02370"
     CDS             4834..>5096
                     /locus_tag="VF453_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686518.1"
                     /translation="MSTPSPLLPLEHLHPALWRAHQLGRGRASVQPSGYRMLDLQLPG
                     GGWPHRALTELLLAAPGWGEMRLLAPALARIGAAGGGVLLIGPP"
BASE COUNT          838 a         1805 c         1741 g          712 t
ORIGIN      
        1 gcgtccggcg cgggccgggc atccgtctcg cgctcgcgca ggacggggat ggcctcgctg
       61 gccgcgagga cgccggggcg ggcgagcact tgaccgggca tcgggactgg gatcctggga
      121 tgagtgaggc tctaaaagag aaccattctc attcattgta ggccttgccc tcgcaggtgt
      181 catccgcagg acacgcaaac tcaccacacg cggcacggct atcggtgcaa ttgaaactat
      241 caatttctac attttgatag ctcgaatcac aatcgagtcc tgcagcgagg cgctctgccg
      301 tcgagccaag gcgaaagcct atcggatgaa tagaaattgg caatcgaatg tctttaagta
      361 atagctctta atcgactaga ctgccccggt cttcaaccca ctgacaagga aagcccatgt
      421 ccctgatcaa cacccaagtc cagcctttca agaccgaagc cttccacaac ggcaagttcg
      481 tcaccgtcag cgacgagacg ctgaagggga agtggtccgt gctgatcttc atgccggccg
      541 ccttcacctt caactgcccg acggaagtgg aggacgccgc cgacaactac gccgaattcc
      601 agaagctcgg cgccgaggtc tacatcgtca ccaccgacac ccacttctcg cacaaggtgt
      661 ggcacgagac ctcgcctgcg gtcggcaagg cgaagttccc gctcgtcggc gacccgacgc
      721 acacgctgac caacgcgttc ggcgtgcaca tccccgagga aggcctcgcg ctgcgcggca
      781 ccttcatcat caatccggaa ggcgtcatca agaccgccga gatccacagc aacgagatcg
      841 cccgcgacgt gaaggaaacg ctgcgcaagc tcaaggccgc ccagtacacg gccgcgcacc
      901 cgggtgaagt ctgcccggcc aagtggaacg aaggcgcgaa gacgatcgcc ccgagcctcg
      961 acctggtcgg caagatctaa cttctctctc agtcgaaaca acgaaagggg ctccacatgc
     1021 tcgattccaa cctgaaggcg cagctcaagg cctacctcga gcgcgtgaag ctgcctttcg
     1081 tcatcacggc ctcgctcgac gaaggcccgt cctcgcagga gatgcacgag ttcctgcagg
     1141 acatcgtcgg cctgaccgac aagatcaccc tgaagaccga cggcacggcc gcgcgccggc
     1201 cgtcgttctc cctggcgcgc gcgggcgagg cggcgcgcat cgagttcgcc gcgatcccga
     1261 tgggccacga gttcacctcg ctcgtgctgg cgctgctgca ggtcggcggc cacccgccca
     1321 agatcgagca ggagctgatc gaccagatca agggactcga aggcgacctg cgcttcgaga
     1381 cctacatgtc cctcacgtgc cacaactgcc cggacgtcgt ccaggcgctg aacctgctgg
     1441 ccgtgctgaa cccgcgcatc cagagcgtgg tcatcgacgg cggcctgtac cagcaggaag
     1501 tcgccgagcg cgacgtgctg gcggtgccga tggtcttcct caacggccag cacttcggcc
     1561 agggccgcat ggacgtcgag gagatcgtca agaagctcga caccggctcg gcggcgcgcg
     1621 atgccgcgaa gctgaaccag aaggacgcct acgacgtgct gatcgtcggc ggcggcccgg
     1681 ccggcgcggc cgcggccgtc tacgcggcgc gcaagggcat ccgcacgggc ctgctggctg
     1741 agcgcatggg cggccagacc atggacacga tgagcatcga gaacttcatc tcggtgctcg
     1801 agaccgacgg cccgaagttc gccgcggcgc tggagcagca tgccaagcgc tacgaggtcg
     1861 acatcctgaa cctgcagcgc gcgcagaagc tcgagccggc gggcgccgac ggcctgctcg
     1921 gcgtcgagct ggccaacggc gcgaagctgc gcagcaagac ggtcatcgtg gccaccggcg
     1981 cgcgctggcg cgaggtcaac gtgccgggcg agaaggaatt ccgcaacaag ggcgttgcat
     2041 attgcccgca ctgcgacggc ccgctgttca agggcaagag cgtcgcggtc atcggcggcg
     2101 gcaactcggg cgtcgaggcg gcgatcgacc tcgccggcat cgtcgcccac gtcgacctgc
     2161 tggagttcgg cgagcagctg cgtgcggacg cggtgctggt caacaagctc gggagcctgc
     2221 ccaacgtcgc gatccacacg cgggcgcaga cgacctcgat cgacggcacg gacggcaagg
     2281 tcagcggcct gacctacacc gaccgcgcca cgggcgagtc gcgcaagctg gcgctcgagg
     2341 gcgtgttcgt gcagatcggc ctggtgccca actccgagtg gctcaagggc acgctggagc
     2401 tcacgaagca cggcgagatc gtcaccgacg cgcgtggcca gacctcgctg cccggcgtgt
     2461 tcgccgccgg cgacgtgacg acggtgccgt tcaagcagat catcatcgcg accggcgacg
     2521 gcgcgaaggc ggcgctcggc gcgttcgacc acctgatccg cagctcggcg ccggtcgcgg
     2581 cctgatcctg cgcgaagccc ggccccgcgc gccggggccg tcgccgtatc ggacgaggcc
     2641 cggcgcaccc gggccacgcc gatttccggc ggccggtcag ccgcgcgcgg gccgcgccac
     2701 ggccggcacc gcctgctggc gccgcatcca caggaagccg tcgaggctgt agacggccag
     2761 gcttgcccag atcagcacga agccggcgag gcggtgcgcg tccaggggct cgccgaacca
     2821 ccatacgccc agcccgaact gcagcgacgg cgacaggtac tgcagcgtgc cgagcgtgac
     2881 catcgtgact cggcgcgcgc cggccgcgaa cagcagcagc gggatcgccg tcaccgggcc
     2941 cgcggccagc agcatcgcca cggtgcccgc cgaaaagccg gtgaagacgc cgccgtggcg
     3001 cgcccccagc acggccatcg cggcgatcgc caccggcgcg agcaccagcg tctccatcgc
     3061 gaggccttcc agcgcgccca gcggcgccgt cttgcgcagc aggccgtagc agccgaagct
     3121 cagcgcgagg ccgaacgcga tccagggcgg acgcccgatc gtgaccgtca gccacgcgac
     3181 ccccagcgcc gcgatgccca ccgcggccca ctgcagtcgg cgcgggcgct cgcgcagcac
     3241 ggcggtgccc agcagcacgt tgaccagcgg cgtgacgaag tagcccaggc tcgcctcgat
     3301 cacgtggccg ttgttgaccg accagatgta gagcagccag ttcccgctca gcagcagggc
     3361 gctcagcagg aactggcccc acagccgcgg cgagcgcgcg acggcgccga gccattgcca
     3421 gcgccgcgtg gccgcgagca ccgcccagat gaacgcgaac gaccacagcg agcggtgcac
     3481 gaccagctcg aacgcgtcga cctgctcgag ggcgtggaag tagagcggga acaggcccca
     3541 caggaagtag gcggaggcgg cgtaggcgag accgggagac atcgcggcat tgtccccgcc
     3601 attcggggga ttggccttct cggccgcagc ctgaaaaatc cctgtcatcg ccgcgacgca
     3661 agattgcggc accggggagc ttcaccatgc cgcaaacgtt caacgtcgga tcccgatcgc
     3721 gcttcgtcaa cctcgtggcg tggctggcca tcctgctcgg cgccttcgcc tgcgcctggg
     3781 cggcgatcca gaacgcggcg cattcgtcct gggcgccggc cgccgccgcc cgcgacggcc
     3841 tgccatggct ctccgggctg ctgctgcgct acctgccctg ggtcttctcg tcggcggtcg
     3901 cgctgtcgct ggcgacgctc gtctgcgcgg tcggcctgct gcagcgcgtg gaatgggcgc
     3961 ggcggctgtt catcggcctg ctggcggtgg cgatcgtggt ggtgctgggc ggcgtctggc
     4021 tgcagcagga gcttgcccat ctcgtggtcg acgacaccct gcgccacgcc gcgctgtcgc
     4081 agcgcgcggc cgagctgttc ggcggcatgg tgatcgtcac gcgcgcgctc gcgggcgccc
     4141 tcagcgtgac ggcggggctc gccctcgcgg cggtgatgcg gcggctgatg tcgccggcgg
     4201 tgcgccagga attcgcctga ccgttcagcc gacatccccg ctgacgtaga accagcggcc
     4261 gccttcccgc acgaaccggc tcgtctcgtg cagtcgctgg gcgcggccac ccagcttgct
     4321 gcgtgcgacg aactcgacga tcgcgtggtc gtcgtcctgg cggacgtggc gccgcacctc
     4381 caggcccagc cagcgcaggc cggcgggcag cggctcgagc tcggccggac gcgtcgaggg
     4441 atgccaggtc tcgcgcaggt aatcgagcag gccggccgcg aaggcgctgt accgcgagcg
     4501 catcaggtcc tcggccgtgg gcgcgcgcag ggcgtccggg ccggcgtgcc agcggccgca
     4561 gcaggccgcg taggcgagcg ggcggccgca ggggcagggt gaatcggaag cgatcggcat
     4621 atccgtggat tctgcccgga tggccgtttc cggaccttgg caggccggtc ggcggtccgg
     4681 tatgctgtat gcaaatacag tacttcgagg gtctcgcgat cgtgtccgcg gcggtcgtcc
     4741 ggctggctgg ccggggcgat ggcttcgccg tccccgcacg cgaccgcgcc gccctcgcca
     4801 accccgtgca ttgtccgccc tccggttccc gcgatgtcga cgccttctcc cctgctgccg
     4861 ctggagcacc tgcatccggc gctgtggcgc gcgcaccagc tcgggcgcgg gcgggcgtcg
     4921 gtgcagccca gcggctaccg gatgctcgat ctccagctgc ccggcggcgg ctggccccac
     4981 cgggcgctga cggagctgct gctcgccgcc cccggatggg gtgagatgcg cctgctcgcc
     5041 cccgcgctgg cccgcatcgg cgcggccggc ggcggcgtgc tgctgatcgg cccgcc
//
LOCUS       DASVLN010000111         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_113053, whole genome shotgun sequence.
ACCESSION   DASVLN010000111 DASVLN010000000
VERSION     DASVLN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113053"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VF453_02375"
     CDS             complement(<1..422)
                     /locus_tag="VF453_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX7686519.1"
                     /translation="MTLSVVYSRALVGLHAPEVQVEVHLANGLPSFTLVGLADTEVKE
                     ARERVRAALAQSGFAFPHNKRITVNLAPADLPKESGRFDLPIALGVLAAQGLLDAERL
                     ARCEFAGELSLAGELRPVRGALALALAVREAGCARRLVL"
     gene            complement(515..1021)
                     /locus_tag="VF453_02380"
     CDS             complement(515..1021)
                     /locus_tag="VF453_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686520.1"
                     /translation="MPHPLSTLGAAHTALSLVPLAVGLREFVRFGGIDPARRAGRVYL
                     GGLALSVFTSFGLSSTGGFNPGHALGILALGAAFGGALAARRAPQARVAPYLAAFGLS
                     FSFFLLLVPGINETLTRLPPAHPLADGPASPVVRSALLAWLAVFACGFVLQVRRLRRA
                     DRPANPGI"
     gene            complement(1097..1681)
                     /locus_tag="VF453_02385"
     CDS             complement(1097..1681)
                     /locus_tag="VF453_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7686521.1"
                     /translation="MFSAPSPKAGEIVACAQALLASGGYHGFSYADISDAVGITKASI
                     HHHFPSKAELVRTVVRQYRESARAGLAGLEERFDDPLAQLRAYTGYWASCLRERASAF
                     CICVMLASELPAIPTEVAQEVRGHFSDLATWLASVLERGAAGRTLRLRDEPRAEAMAL
                     MAAVHGAMLSARALGDPEVFARTVEPALQRLRPA"
     gene            2185..3000
                     /locus_tag="VF453_02390"
     CDS             2185..3000
                     /locus_tag="VF453_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX7686522.1"
                     /translation="MKTSALAHALALTAGLAATVAPTLAHADDAPASPITYNIGAVTD
                     YKYRGITQTKFEPAVQGGVDYAQGAIYAGTWLSTIGWVKDAAKAYNNANGTDVKSNAP
                     LEWDLYGGTKGDIVKDTLSYDVGVLAYVYLGQHFHQIGLANPDTGEAYVALTSGIFTG
                     KVSVSATDLFGVPKSKGSQYYDLTANVDLGSGFTLTPEIAYQNITGHFFQDAGLPDPS
                     YALFSATLAKDFGKGLSASLQVVGTDAKKEDYQTSSGKFTGRTGVVAGIKYSF"
     gene            3083..3421
                     /locus_tag="VF453_02395"
     CDS             3083..3421
                     /locus_tag="VF453_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7686523.1"
                     /translation="MKMVTAVVKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAVDDALVDQVIEAIETSARTGKIGDGKIFVSALEQVVRI
                     RTGETGKDAL"
BASE COUNT          601 a         1319 c         1247 g          450 t
ORIGIN      
        1 aggacgagcc ggcgcgcgca gcccgcttcg cggaccgcca acgcgagcgc gagcgcgccc
       61 cgcacgggtc gcagttcgcc ggccagcgag agctcgcccg cgaactcgca gcgcgccagg
      121 cgctccgcgt cgagcaatcc ctgcgccgca agcacgccca gcgcgatcgg caggtcgaag
      181 cgaccggact ccttgggcag gtcggccggc gcgaggttga cggtaatgcg cttgttgtgc
      241 gggaaggcga agccgctctg ggcgagcgcc gcgcgcacgc gctcgcgggc ttccttcact
      301 tcggtgtccg cgaggccgac cagcgtgaag ctgggcaggc cgttggcgag atgcacctcg
      361 acctgcacct cgggcgcgtg caggcccacg agggcgcggc tgtagacgac ggacagggtc
      421 atggaactcc ggctggaacg tggcggcccg gcccggttgc cacctgcgcc ggagccgtct
      481 cgattctcga gcgggcccgt ggaccctgcc cgcgtcaaat gccggggttt gcggggcgat
      541 cggcgcgccg cagccggcgc acctgcaata cgaagccgca ggcgaagacc gcgagccagg
      601 cgagcagtgc agagcgcacg accggcgagg ccggcccgtc cgccagcggg tgcgcgggcg
      661 gcaggcgagt cagcgtctcg ttgatgcccg gcaccagcag gaggaagaag ctgaacgaca
      721 ggccgaacgc ggccagatag ggcgccacgc gggcctgcgg cgcccggcgc gccgccagcg
      781 ccccgccgaa tgcggcgccc agcgcgagga tgcccagcgc atggccggga ttgaatccgc
      841 cggtgctcga gagcccgaag gacgtgaaca ccgacagcgc gaggccgccg aggtagaccc
      901 gccccgcgcg ccgcgccgga tcgattccgc cgaagcggac gaactcgcgc aagccgacgg
      961 ccaggggaac caggctgagc gcggtgtgcg cggcgcccag ggtcgagagg gggtggggca
     1021 tgatgcagtc ctttcgatta tctacctacc agttggtaga ctattggtgc aaaaaaaatg
     1081 cgaagcgtcc gggcgctcag gcgggccgca gccgctggag cgccggctcg acggtcctcg
     1141 cgaagacctc ggggtcgccg agtgcgcgcg ccgacaacat cgcgccgtgc acggccgcca
     1201 tgagggccat cgcctccgcg cgcggctcgt cgcgcaggcg cagcgtgcgg ccggcggcgc
     1261 cgcgctcgag caccgaggca agccacgtcg cgagatccga gaagtgcccg cgcacctcct
     1321 gcgcgacctc ggtcgggatc gccgggagct ccgacgcgag catcacgcag atgcagaacg
     1381 ccgacgcccg ctcgcgcagg cagctcgccc agtacccggt gtaggcgcgc agttgcgcca
     1441 gcggatcgtc gaaccgctcc tcgaggccgg cgaggccggc gcgcgcgctc tcgcggtatt
     1501 gccggacgac cgtccgcacc agctccgcct tgctcgggaa gtggtggtgg atgctcgcct
     1561 tggtgatgcc cacggcgtcg gagatgtcgg catagctgaa gccgtggtag ccgccggatg
     1621 cgagcagcgc ctgcgcgcag gcgacgatct ctccggcctt gggcgatgga gcggaaaaca
     1681 tgcgctagtc taccaactag tagattgatg tcaaggatca tgcgacgctt ccgccgcgga
     1741 tcgccgcgcc cgaggaaacg gggacaggat cgcgcgccgc aagctgaacc ggagctggcc
     1801 cgcgatttgc gtggccccaa ttcggaacat gccgatccgg ccacaggcac cgtcctggac
     1861 gccgccgggt gccgcggccg tccctgcgca cgcccattcg tgcgggtgcg ggcccacgag
     1921 cacgaacgtc gccccaaacg ggtgcacccg catcgtgcgt gtgcggaaac gcccgcccgg
     1981 cttcggtgcg cgtgacgagg tcgggcgcac gtcccttggc gcgccgccgc gcgggttggt
     2041 gcaggacggt cccctggctc ggcgcccgcg tccgccgggg cagctcgacc gcgtggcacg
     2101 gaacctgcgt caacgcctgc aaagacggcc gcgaaggccc tgccggttcc atccacccga
     2161 agcccaccta cccggagatc actgatgaag acatccgcgc tcgcccacgc gctcgccctc
     2221 acggccggcc tcgccgcgac ggtcgccccg accctcgcgc atgccgacga cgcgcccgcc
     2281 agcccgatca cctacaacat cggcgcggtc accgactaca agtaccgcgg catcacgcag
     2341 accaagttcg agccggccgt ccagggcggc gtggactacg cgcagggcgc gatctacgca
     2401 ggcacctggc tgtccacgat cggctgggtc aaggacgccg cgaaggccta caacaacgcc
     2461 aacggcacgg acgtgaagag caacgccccg ctcgaatggg acctgtacgg cggcaccaag
     2521 ggcgacatcg tcaaggacac gctgtcctac gacgtgggcg tgctcgcgta cgtctacctg
     2581 ggacagcact tccaccagat cgggctggcc aacccggaca ccggcgaagc ctacgtcgcg
     2641 ctgacctcgg gcatcttcac gggcaaggtc tcggtgagcg cgaccgacct gttcggcgtg
     2701 cccaagagca agggcagcca gtactacgac ctgaccgcca acgtcgacct cggcagcggc
     2761 ttcacgctga cgcccgagat cgcgtaccag aacatcaccg gccacttctt ccaggacgcg
     2821 ggcctgcccg acccgtcgta cgcgctgttc tccgcgacgc tcgccaagga cttcggcaag
     2881 ggcctgtcgg cctcgctgca ggtcgtcggc accgacgcca agaaggagga ctaccagact
     2941 tccagcggca agttcacggg ccgcaccggc gtggtcgccg gcatcaagta ctcgttctga
     3001 cgcatcccgc ggcccggggc gccgggccgc attgttcgac acggggcgcc cgcacggcgc
     3061 ccgccagcct ggagatacga ccatgaagat ggtgaccgcc gtcgtgaagc ccttcaagct
     3121 cgacgaagtc cgcgaggccc tgagcgcgat cggcgtgcag ggcatcacgg tgaccgaagt
     3181 gaagggcttc ggccgccaga agggccacac cgagctgtac cgcggcgccg agtacgtcgt
     3241 ggacttcctg cccaaggtca agatcgaggc ggccgtcgac gacgcgctcg tcgaccaggt
     3301 catcgaggcc atcgagacct ccgcccgcac cggcaagatc ggcgacggga agatcttcgt
     3361 ctccgccctc gagcaggtcg tgcgcatccg taccggcgag accggcaagg acgcgctctg
     3421 acgccgcgcc cgcccgcccc gtccctggct tcccacactc cctgaaagct gaaagcaccg
     3481 caaccatgag acagctgatc gcttccctcg ccctggggct cgccgccctg gggttcagcg
     3541 cgacctcgtt cgcggcggac gacgcggcct ccgcgccggc cgcgaccgcc tccgccgccg
     3601 cgcccgccgc ctccgcc
//
LOCUS       DASVLN010000112         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_167256, whole genome shotgun sequence.
ACCESSION   DASVLN010000112 DASVLN010000000
VERSION     DASVLN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167256"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VF453_02400"
     CDS             complement(<1..963)
                     /locus_tag="VF453_02400"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7686524.1"
                     /translation="MSTAARAAAPPLPYVTIALVALNVLAYVLELTLGVPSYTGAPGP
                     LVAAAGNAPVFTLGGQPWRLATGMFLHGGLLHLGMNMMSLGIAGRRSEEEFGHARTLL
                     VYLGGGLLASCASAVWSGHRVLALDAAGFAALHAGLSVGASGAVMAVFGALVAAALFA
                     LPTFDLPDPRPMVDWPLLGLIALTVASGWFSPLVYRAAHAAGPAAGPLLQAAYWIGLL
                     VLAALGFAALRAWREGSEQSDERLLNLAMLAMVVLNVLVCLSVPTAVDQAAHVGGVVA
                     GFALGALLAVGRGRGTALVTGLRALACVGLVAGGIHVALSWGERN"
     gene            1147..1458
                     /locus_tag="VF453_02405"
     CDS             1147..1458
                     /locus_tag="VF453_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEX7686525.1"
                     /translation="MPPSDQRPESGPDAAARARHWRRTRGLTAVLLAAWFGVGFVVPW
                     FARDLDFRFFGWPFGFWASAQGGVILFVALIAVYARRMRRVDRLLDARPGPAPGGDPP
                     P"
     gene            1455..>2402
                     /locus_tag="VF453_02410"
     CDS             1455..>2402
                     /locus_tag="VF453_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HEX7686526.1"
                     /translation="MSLRTRTFAPARSRLRPISGAAFSQRLHWIYVAFGVAFVLFVAA
                     MAGLERLGLPRPWIGFLFLVGPVAFYAGIGILSRTTDPAEYYVAGRRVPAIYNGMATG
                     ADWMSAASFIGMTGTLYLSGYGGLAYTLGWTGGFVLVALLIAPYLRKFGQYTVPDFLA
                     ARYDGNLVRVIGVVAAIGCSFLYVVAQIYGVGLITSRITGVAFEVGVFLGLGGILVCS
                     FLGGMRAVTWTQVAQCIVLLVAFLVPVAWLSLGQGAGVVPQASYGYQLQQVIAAEKRL
                     QADPAERQVMAVYRARAAVYDAKLADIPAALAADRRAAAK"
BASE COUNT          297 a          917 c          876 g          312 t
ORIGIN      
        1 gttgcgctcg ccccaggaca gcgcgacgtg gatgccgccc gcgaccagcc cgacgcaggc
       61 gagcgcgcgc aggccggtca ccagcgccgt gccgcgaccg cggccgacgg ccagcagcgc
      121 gccgagcgcg aagccggcga cgaccccgcc gacgtgcgcg gcctggtcga ccgcggtggg
      181 caccgacagg cagaccagga cgttgagcac caccatcgcg agcatcgcga ggttcagcag
      241 ccgctcgtcg ctctgctcgg agccttcgcg ccaggcgcgc agcgccgcga agcccagggc
      301 cgctagcacc agcaggccga tccagtaggc cgcctgcagc agcggcccgg cggccgggcc
      361 ggcggcgtgg gccgcccggt acaccagcgg cgagaaccag cccgacgcga ccgtcaacgc
      421 gatcaggccg agcagcggcc agtccaccat cggccgggga tcgggcaggt cgaaggtcgg
      481 cagcgcgaac agcgcggcgg ccacgagggc gccgaagacg gccatcaccg cgcccgaggc
      541 gccgaccgac agccccgcgt gcagcgccgc gaagccggcg gcgtcgagcg ccagcacgcg
      601 atggcccgac cacacggcgc tcgcgcagct cgcgagcagc ccgccgccga ggtagaccag
      661 cagcgtgcgc gcgtggccga actcctcttc cgagcggcgg ccggcgatgc cgagcgacat
      721 catgttcatg cccaggtgca gcagcccgcc gtgcaggaac atgccggtgg ccaggcgcca
      781 gggctggccg cccagcgtga agacgggcgc gttgcccgcc gcggccacga gcggccccgg
      841 cgcgccggtg taggacggca cccccagggt cagctccagg acgtaggcga gcacgttcag
      901 cgccacgagc gcgatcgtca cgtagggcag gggcggggcg gcggcgcgtg cggcagtgga
      961 catggcggac ttcgatcggg gatgacccgc cgggttccgc ggcgggcggc ggcggacggc
     1021 cgtcggcgat gatcggcacc gcgcgtcggg tttggcccgc ttgggcgggc gccaccgccc
     1081 gggcatcatg ccagtcgcgg cgcccgatgc gccagacgca cctccatcgt tccagccgat
     1141 ccccgcatgc cgccctccga ccagcgaccc gaatccggcc ccgacgccgc cgcgcgcgcc
     1201 cgccactggc gccgcacgcg cggcctgacc gccgtcctgc tggcggcctg gttcggggtc
     1261 ggcttcgtcg tgccctggtt cgcgcgcgac ctcgacttcc gcttcttcgg ctggccgttc
     1321 ggcttctggg ccagcgccca gggcggcgtg atcctgttcg tggcgctgat cgccgtctac
     1381 gcgcggcgca tgcgacgcgt cgatcgcctg ctcgatgcgc ggcccgggcc ggcccccggc
     1441 ggcgaccccc cgccatgagc ctgcgcaccc gcaccttcgc cccggcgcgc agccgcctgc
     1501 gccccatcag cggcgccgcg ttctcgcagc ggctgcactg gatctacgtc gcgttcggcg
     1561 tcgccttcgt gctgttcgtc gcggcgatgg cggggctgga gcgcctcggg ctgccacggc
     1621 cgtggatcgg cttcctgttc ctcgtcggcc cggtggcgtt ctacgccggc atcggcatcc
     1681 tgagccggac gaccgacccc gccgagtact acgtcgccgg ccgccgcgtg ccggcgatct
     1741 acaacggcat ggccaccggc gcggactgga tgtcggcggc ctcgttcatc ggcatgaccg
     1801 gcacgctcta cctcagcggc tacggcgggc tcgcgtacac gctcggatgg acgggcggct
     1861 tcgtgctggt cgcgctgctg atcgcgccgt acctgcgcaa gttcggccag tacacggttc
     1921 ccgacttcct cgccgcgcgc tacgacggca acctcgtgcg cgtgatcggc gtggtcgcgg
     1981 cgatcggctg ctcgttcctg tacgtggtgg cgcagatcta cggcgtcggg ctgatcacga
     2041 gccgcatcac cggcgtcgcg ttcgaggtcg gcgtcttcct cgggctgggc ggcatcctcg
     2101 tgtgctcgtt cctcggcggc atgcgggcgg tgacctggac gcaggtcgcg cagtgcatcg
     2161 tgctgctggt ggccttcctc gtgccggtcg cgtggctctc gctcgggcag ggcgccggcg
     2221 tcgtgccgca ggcgagctac ggctaccagc tgcagcaggt catcgccgcc gagaagcggc
     2281 tgcaggccga tcccgccgag cgccaggtga tggcggtcta ccgggcgcgc gccgccgtct
     2341 acgacgccaa gctcgccgac atccccgccg cgctcgccgc ggatcgccgt gccgcggcca
     2401 ag
//
LOCUS       DASVLN010000113         4067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_167516, whole genome shotgun sequence.
ACCESSION   DASVLN010000113 DASVLN010000000
VERSION     DASVLN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167516"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VF453_02415"
     CDS             complement(<1..456)
                     /locus_tag="VF453_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686527.1"
                     /translation="MERHPPETPTEAYAREVADRFGGLPHFFCSAQVAPGLGEGLWGF
                     ARSAYLDNPLPSAFKERLFVHLSRFCASRFCLVRHASFLMGHGRPAGVDGPVAGVDEV
                     IALLERPIPDAAALAGALQRLERGPALDELPLPGQPAEHDLLDALSAVFV"
     gene            697..1158
                     /locus_tag="VF453_02420"
     CDS             697..1158
                     /locus_tag="VF453_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686528.1"
                     /translation="MRYRHFGRHHGHRSHSRSGGWPAVLAISLEGQRLRRRLVFGGGL
                     VIAGILWLLERHGALAADELWLVAPAVLAWSAAVRLAIRPSALSVVGAFVRLALAAYL
                     VTVIEHLGGWTLAQTWPVLLIGYGLAHVAGALAWRACAGAGAGDASEEPTW"
     gene            1155..1889
                     /locus_tag="VF453_02425"
     CDS             1155..1889
                     /locus_tag="VF453_02425"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX7686529.1"
                     /translation="MKPDLGHRQVGRIVFGLFIIVVGLFALLDNLHLFDSHLVQPYWP
                     LVFVALGALKLGQNDHPRRRWFGAVLIAVGAAMTANNLGLVRFQVRDWWPLLLVGVGL
                     LVIFKRGDGPRGRWRHRHRGGMGCDGGHDERVEHGSRVDASAVMSGIVLKNDSQDFQG
                     GEISTVMGAVEVDLRQASIAGEARLHLSVIMGGVEIKVPRDWSISIAGTPTLGGIEDK
                     TIPPMAPGKRLVIEGSVVMGGVEISN"
     gene            1892..2983
                     /locus_tag="VF453_02430"
     CDS             1892..2983
                     /locus_tag="VF453_02430"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7686530.1"
                     /translation="MHPLLADVRKLFWYVLAWELAGIGIAALLHLAGQARWLGALTFA
                     LPVCGVYAFAALSSYYVCRSLPWAQRRWPVALALFGGAALLSALAWLGLGEAWSAVGR
                     LGDRNAPLVALSSAGWVLYFVAGFVLYLLSLLAHDVLIAFETVQDAARREAESRVLAR
                     DAELQMLRTQINPHFLFNSLNSISALTSIDPAAARDMTIDLAQFFRRTLALAERHRIA
                     LGEELALSEHFLSIEQRRFGAKLRAELRVAPEAAGCLLPPMTLQPLLENAIKHGIRQL
                     DEGGTITVEAVVRDGWLHVSVDNPVGDVADAGARGHGMGLRNIRERLAALYGSRAYIA
                     WGRAEGRFTAEITLPAERQAPPNQQEQRP"
     gene            2980..3741
                     /locus_tag="VF453_02435"
     CDS             2980..3741
                     /locus_tag="VF453_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7686531.1"
                     /translation="MTTTSTGPLRTLIVDDEALARALVREYLQGHADIEVVGECENGL
                     DAVRQIGALAPDLVFLDIQMPRLTGLEVLELTGRRGGVIFTTAYDEHALKAFELHAVD
                     YLLKPFSKTRFDAALERARALHASAAAMAASSQALDALVARKAEPIERILIRDREQVH
                     VIPVGKVEYLEAQGDYLAVISEGRTHLKPQRISEIEEQLDPRHFLRVHRSFIINLAHL
                     TSIERQGADRHVARMRSGKGVPISRSGYEKLRTLV"
     gene            complement(3745..>4067)
                     /locus_tag="VF453_02440"
     CDS             complement(3745..>4067)
                     /locus_tag="VF453_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7686532.1"
                     /translation="HRWAAGDEPDGPRIAAFAGVSLVVNGLVLGVLAPYRHSTQAYLK
                     ATWIDTRADVLVNAGVLVSGALVALTGYRAIDLVAGAVLAVFVVHEGWEIWESPGEEP
                     SAGD"
BASE COUNT          542 a         1484 c         1474 g          567 t
ORIGIN      
        1 gacgaacacc gccgagagcg cgtcgagcag gtcgtgctcc gccggctgtc cgggcagcgg
       61 cagctcatcc agggccggcc cgcgttccag gcgctgcagc gcgccggcga gcgccgcggc
      121 atcggggatc ggccgctcca gcagcgcgat cacctcgtcg acgcccgcga ccggcccgtc
      181 cacgcccgcg ggacgcccgt gccccatcag gaagctggcg tggcggacga ggcagaagcg
      241 cgaggcgcag aagcgcgaca ggtggacgaa cagccgctcc ttgaacgccg agggcagcgg
      301 attgtccagg taggccgagc gcgcgaagcc ccacagcccc tcccccagcc ccggcgccac
      361 ctgcgccgaa cagaagaagt ggggcaggcc gccgaagcgg tcggcgacct cgcgcgcgta
      421 ggcctcggtc ggggtctcgg gagggtggcg ttccatggag cgcatcgtgc gggcgggccc
      481 gatgccccga atgtatcgcg atgcctcgcc cgggcaagcg gcgggcgcat cgcggcccga
      541 cgggtggatc cggcgattcg tcgacgcccc gccccggccg gcgccccgcc cgcccgttcg
      601 tcgcccatcc ccgccggttc gtcgattgca agatgcctgc gggcccgaca gctacgatga
      661 ttcgtaccca gcaagaccat ccgcctggat ccgaccatgc gctaccgcca cttcggccgc
      721 catcacggcc accgctccca ctcccgttcc ggcggatggc ccgccgtgct ggcgatctcg
      781 ctggagggcc agcggctgcg ccggcggctc gtcttcggcg gcgggctggt gatcgccggg
      841 atcctctggc tgctcgagcg gcacggcgcc ctggccgcgg acgagctgtg gctggtcgcg
      901 cccgccgtgc tcgcttggtc ggcggccgtg cggctggcga tccggccgtc ggcgctgtcg
      961 gtcgtcggcg ccttcgtccg gcttgcgctc gcggcctacc tcgtgacagt gatcgagcac
     1021 ctgggcggct ggaccttggc gcagacctgg cccgtgctgc tgatcggcta cgggctcgcg
     1081 cacgtcgcgg gcgcgctggc ctggcgcgcg tgcgccggcg ccggcgcggg cgacgcgagc
     1141 gaggagccga cgtggtgaag cccgacctcg gccatcgcca ggtgggccgc atcgtcttcg
     1201 gcctgttcat catcgtcgtc gggctgttcg cgctgctgga caacctgcac ctgttcgaca
     1261 gccacctcgt gcagccgtac tggccgctgg tgttcgtggc gctgggcgcg ctgaagctgg
     1321 gccagaacga ccacccgcgc cggcgctggt tcggggcggt gctgatcgcc gtcggcgccg
     1381 cgatgacggc caacaacctc ggcctggtcc gcttccaggt gcgcgactgg tggccgctgc
     1441 tgctggtcgg cgtcggcctg ctggtgatct tcaagcgcgg cgacggcccg cgcggccgct
     1501 ggcgccaccg ccaccgcggg ggcatgggct gcgacggcgg tcacgacgag cgcgtggagc
     1561 acggctcgcg cgtcgacgcc tcggcggtga tgagcggcat cgtgctgaag aacgactcgc
     1621 aggacttcca gggcggcgag atcagcaccg tgatgggcgc cgtggaggtc gacctgcgcc
     1681 aggcctccat cgccggcgag gcgcggctgc acctgtcggt gatcatgggc ggcgtcgaga
     1741 tcaaggtgcc gcgcgactgg tcgatctcca tcgccggcac gccgaccctg ggcggcatcg
     1801 aggacaagac gatcccgccg atggcaccgg gcaagcgcct cgtcatcgag ggctccgtcg
     1861 tcatgggcgg cgtggagatc agcaactagg catgcacccg ctgctcgcgg acgtccgcaa
     1921 gctcttctgg tacgtgctcg cgtgggagct cgcgggcatc ggcatcgccg cgctgctgca
     1981 cctggccggg caggcgcggt ggctcggcgc gctcaccttc gcgctgccgg tctgcggggt
     2041 gtacgcgttc gcggcgctgt cctcgtacta cgtctgccgc agcctgccct gggcgcagcg
     2101 gcgctggccg gtggcgctgg cgctgttcgg cggcgcggcg ctgttgtcgg cgctcgcctg
     2161 gctgggactg ggcgaggcct ggagcgccgt cggccggctg ggcgaccgca acgcgccgct
     2221 ggtggccttg agctcggcgg gctgggtgct gtacttcgtc gcgggcttcg tgctgtacct
     2281 gctgtcgctg ctcgcgcacg acgtgctgat cgcgttcgag accgtgcagg acgccgcgcg
     2341 ccgcgaggcc gagtcgcgcg tgctcgcgcg cgacgccgag ctgcagatgc tgcgcacgca
     2401 gatcaacccg cacttcctgt tcaacagcct gaactcgatc agcgccctca cgtcgatcga
     2461 tcccgccgcc gcgcgcgaca tgacgatcga cctcgcgcag ttcttccgcc gcacgctggc
     2521 gctcgccgag cgccaccgga tcgcgctggg cgaggagctc gcgctcagcg agcacttcct
     2581 gtccatcgag cagcgccgct tcggcgccaa gctgcgcgcc gagctgcgcg tggcgcccga
     2641 ggccgcaggc tgcctgctgc cgccgatgac gctgcagccg ctgctggaga acgcgatcaa
     2701 gcacggcatc cgccagctcg acgagggcgg cacgatcacg gtcgaggcgg tggtgcgcga
     2761 cggctggctg cacgtgtcgg tggacaatcc ggtgggcgac gtggccgacg ccggcgcccg
     2821 cggccacggc atgggcctgc gcaacatccg cgagcgcctg gccgcgctct acggcagccg
     2881 cgcctacatc gcctggggcc gcgccgaggg ccgcttcacg gccgagatca cgctgcccgc
     2941 cgagcggcag gccccgccga accagcagga acaacgccca tgaccacgac ctccaccggc
     3001 ccgctgcgca cgctgatcgt cgacgacgag gcgctggcgc gcgccctggt ccgcgaatac
     3061 ctgcaggggc atgcggacat cgaggtcgtc ggcgagtgcg agaacggcct ggacgccgtg
     3121 cgccagatcg gcgcgctcgc gcccgacctc gtgttcctcg acatccagat gccgcgcctg
     3181 accggcctcg aggtgctgga gctgaccggc cggcgcggcg gggtgatctt cacgacggcg
     3241 tacgacgaac acgcgctcaa ggccttcgag ctgcacgcgg tcgactacct gctcaagccg
     3301 ttctcgaaga cgcgcttcga cgccgcgctc gagcgcgcgc gcgccctgca cgcctcggcg
     3361 gcggcgatgg ccgcgtcctc gcaggcgctc gacgcgctcg tcgcgcgcaa ggccgagccg
     3421 atcgagcgca tcctgatccg cgaccgcgag caggtgcatg tgatcccggt cgggaaggtg
     3481 gagtacctcg aggcccaggg cgactacctc gccgtgatct ccgaaggccg cacgcacctg
     3541 aagccgcagc gcatctccga gatcgaggag cagctcgacc cgcgccattt cctgcgggtg
     3601 caccgatcct tcatcatcaa cctcgcccac ctgacgagca tcgaacgcca gggcgccgac
     3661 cgccacgtcg cgcgcatgcg cagcggcaag ggcgtgccga tcagccgcag cggctacgag
     3721 aagctgcgga cgctggtata ggcgtcagtc gcccgcggaa ggttcctcgc ccggggattc
     3781 ccagatctcc cagccctcgt gcacgacgaa gacggccagc acggcgccgg cgacgaggtc
     3841 gatcgcgcga tatccggtga gcgcgacgag cgcgcccgac accagcacgc ccgcgttgac
     3901 gaggacgtcg gcgcgcgtgt cgatccaggt ggccttcagg taggcctggg tgctgtgccg
     3961 atacggcgcg agcacgccca ggaccaggcc gttgacgacg agcgagacgc ccgcgaaggc
     4021 ggcgatgcgc gggccgtcgg gctcgtcgcc cgccgcccag cgatgca
//
LOCUS       DASVLN010000114         5865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_435760, whole genome shotgun sequence.
ACCESSION   DASVLN010000114 DASVLN010000000
VERSION     DASVLN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435760"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VF453_02445"
     CDS             <1..638
                     /locus_tag="VF453_02445"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX7686533.1"
                     /translation="AGGALAGRVVERPHRSGPRLLPTGPDGQTMLPWSVHVSDPLPVV
                     LHDMNKVSDNVTARHLMLALARGFPLKSATLPDAQARMREWLATQGLATGDVEVENGS
                     GLSRAERARPRALAHLLANAWHAPRLREFILSLPVAGMDGTLQHRMQGTPAEGHAFLK
                     TGTLDDTRALAGYVSAASGRTYAVALMVNHPEAARATPTLDACIEWLVRHG"
     gene            complement(1019..2236)
                     /locus_tag="VF453_02450"
     CDS             complement(1019..2236)
                     /locus_tag="VF453_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7686534.1"
                     /translation="MSTTSTATASLARDGARPPGATFGILGAISAAHMINDMMQSLIL
                     AIYPILKGEFGLSYGQIGLITLTYQLTASLFQPLVGLYTDRKPVPYSLPFGMAFTLAG
                     LLLLAWAPAYGWVLLAASLVGIGSSIFHPESSRVARMASGGQHGLAQSLFQVGGNTGS
                     ALGPLVAAKVIEPRGQGSVAWFALAALVGIALLSQVSRWYAVRRLATAAKGGTRAAAS
                     PHPRRVVVGAIATLLLLIFSKYFYIASISSFYTFYLIHKFGLSVQSAQMQLFVFLAAS
                     AAGTLVGGPVGDRIGRKPVIWGSILGVAPFALALPHVGLALTTTFTILIGLILSSAFS
                     AILVYAQELIPGKVGAVSGLFFGFAFGMGGLGAAALGLLADATSIDFVYQVCAFLPLL
                     GVVAIALPRTKRA"
     gene            2372..3157
                     /locus_tag="VF453_02455"
     CDS             2372..3157
                     /locus_tag="VF453_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7686535.1"
                     /translation="MTRPTVPTDQRYFTDVDQDPMPVVSKATDYPAGHVIPPHTHVRG
                     QLVYSEHGVVMLTAAGGSWIVPPTRAVWIPAGTLHHLRCIGAVRMRSLYLRPDLTPEL
                     PAHAQAVSVSPLLRELILAAVAVVPPYSMDSRDGRVMRLLLDELQALPVLPLHLPQPA
                     DPRLRRVCERLAAQPDDTSTLADWAAELAVDAKTIQRLFQREAGMTFGQWRQQARLLH
                     ALERLALGDRVLDVALALGYESPSAFATMFKRQFGETPSAFFR"
     gene            3257..4837
                     /locus_tag="VF453_02460"
     CDS             3257..4837
                     /locus_tag="VF453_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7686536.1"
                     /translation="MKFSDLRVKTKLILGFSVLAAVVVLVSVLALRSLGRADDRLSGY
                     LSGIAQRERLAQEIRAAADARAIAARNLVLVTEPSDIALEKAAVTASHAEVGAALARL
                     KAAVAAAPDATARERALLADIERIESQYGPVALDIAAKALDGRKEDATLAMDTRCRPL
                     LAQLLKATSDDIAYARQVADERVAASHASYAGDRAVQATVSTAAVLLALGLGWLLARA
                     VTRPLERAVAIAEAVAGGDLTSSVVVDRKDETGQLLAALGRMNEGLVRMVAQVRESAE
                     GIASASSQIAAGNQDLSSRTEHQASALQQTAASMHQMTSTVQQAAGHARTASQLAAAA
                     AEVAGRGGQAVHRVVSTMGEISASSQRIADIIGVIDGIAFQTNILALNAAVEAARAGE
                     QGRGFAVVAGEVRSLAQRSAAAAREIKALIGASAERVQAGSSLVGEAGATMDDVVAQV
                     RRMTDLMGEIGASSQEQERGIVQVNQAVASIDEGTQQNAALVEQGAAAAESLKQQSVR
                     LLGMVAAFRLADHCAAAA"
     gene            4906..5310
                     /locus_tag="VF453_02465"
     CDS             4906..5310
                     /locus_tag="VF453_02465"
                     /inference="COORDINATES: protein motif:HMM:NF016807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEX7686537.1"
                     /translation="MRAIAYVSTASSNLLREDLEAIVAASRGRNAESGITGVLLYCDG
                     HFMQYIEGPDAAVLEAFERIRRDARHYQVNELLNQAITEREFADWTLGFTRASPATVL
                     DPTTRSWDRPDHDGPGVEMLRVFWRNCRSVTA"
     gene            complement(5396..>5865)
                     /locus_tag="VF453_02470"
     CDS             complement(5396..>5865)
                     /locus_tag="VF453_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7686538.1"
                     /translation="RVLLLAPAAVPFLRQAADNPGGLPQAAIDAHLAALEDDFPAWIE
                     ANSAPYFGAAGNRPLIDWTAGDMLRASMQATVELARLQLHTDFRPELARVDVPVLIVH
                     GTADASAPVELTGRPAAALIPGARLVEIDGAPHGLYVTHRQQVNALIADFARG"
BASE COUNT          827 a         2198 c         2109 g          731 t
ORIGIN      
        1 aggcgggcgg ggcgctcgcg ggccgcgtcg tcgagcggcc gcaccgctcc ggcccccgcc
       61 tgctgcccac cggccccgac ggccagacga tgctgccctg gtcggtgcac gtgtccgacc
      121 cgctgcccgt cgtgctgcac gacatgaaca aggtcagcga caacgtcacc gcgcgccacc
      181 tgatgctggc gctcgcgcgc ggcttcccgc tgaagtcggc gacgctgccg gacgcccagg
      241 cgcgcatgcg cgaatggctc gcgacccagg gcctggcgac gggcgacgtc gaggtggaga
      301 acggctcggg cctgtcgcgc gccgaacggg cgcggccgcg cgcgctcgcg cacctgctgg
      361 ccaacgcgtg gcatgcgccg cggctgcgcg agttcatcct gtcgctgccc gtggccggca
      421 tggacggcac gctgcagcac cgcatgcagg gcacgcccgc cgagggccac gccttcctca
      481 agaccggcac cctggacgac acgcgcgccc tggccggcta cgtcagcgcc gccagcggcc
      541 gcacctatgc cgtcgcgctc atggtcaacc atcccgaggc cgcgcgggcg acgccgacgc
      601 tggacgcctg catcgagtgg ctggtgaggc acggctgagc ccgtgccggg acgtcagttc
      661 cggtgcagcg acgccgccgc ctggtcgtag ctgccaccgt cgtcgaaatg ggtctcctgc
      721 gccgccttcc agccgccgaa ggccgggtcg acggtgaagg tggccacggc gggaactcgc
      781 gcgcgtgcgc ggcgagcacc tgggcgagcc cgggcgcagg ccgtggcggg cggcgagagc
      841 caggcgtagc ggccgttgcc cgaggtcttc ggggtgggga tgacgacggc cgtgccgggt
      901 cgggcgagat gatcgcgatc acctcgccgg ccgcctgctt cccaatgggc ggcgaacgcg
      961 gggtcggagt ccttgtagaa ctcgcgggtg acgtcgcagg gcgggcgcgg gacgcgcctc
     1021 aggcgcgctt cgtccgcggc agcgcgatgg cgaccacgcc caggagcggc aggaatgcgc
     1081 agacctggta gacgaagtcg atgctggtcg cgtcggccag caggcccagc gccgcggcgc
     1141 ccaggccgcc catgccgaac gcgaagccga agaacaggcc cgagaccgcg cccaccttgc
     1201 cggggatgag ctcctgcgcg tagacgagga tcgccgagaa cgccgacgac aggatcaggc
     1261 cgatcaggat cgtgaacgtc gtcgtgagcg cgagccccac gtgcggcagc gcgagcgcga
     1321 acggcgccac gccgaggatc gagccccaga tcaccggctt gcggccgatg cggtcgccca
     1381 cgggcccgcc gacgagcgtg cccgcggccg aggcggccag gaacacgaac agctgcatct
     1441 gcgccgactg caccgacagc ccgaacttgt ggatcaggta gaaggtgtag aagctgctga
     1501 tgctggcgat gtagaagtac ttcgagaaga tcagcagcag gagcgtcgcg atcgcgccga
     1561 cgacgacgcg cctcggatgc ggcgacgccg cggcgcgcgt gccgcccttg gccgcggtcg
     1621 cgaggcgtcg caccgcgtac cagcggctga cctgcgacag cagcgcgatg cccaccagcg
     1681 cggcgagcgc gaaccacgcg acgctgccct ggccgcgcgg ctcgatcacc ttggcggcca
     1741 ccagcggccc gagcgcgctg cccgtgttgc cgccgacctg gaacagcgac tgcgcgaggc
     1801 cgtgctgccc gcccgaggcc atgcgcgcga cgcgcgagga ctccggatgg aagatcgacg
     1861 agccgatccc gacgagcgac gccgccagca gcacccagcc gtaggccggc gcccaggcca
     1921 gcagcagcag gccggccagc gtgaaggcca tgccgaaggg cagcgagtag ggcaccggct
     1981 tcctgtccgt gtacaggccg accagcggct ggaacagcga ggcggtcagc tggtaggtca
     2041 gggtgatcag cccgatctgg ccgtagctca gcccgaactc gcccttcagg atcgggtaga
     2101 tcgccaggat cagcgactgc atcatgtcgt tgatcatgtg ggcggcgctg atcgcgccga
     2161 ggatcccgaa ggtggcgccc ggcgggcgcg cgccgtcgcg ggcgagggag gcggtggcgg
     2221 tggaagtcgt cgacatgggg gtgttgggcg ggcacgccgc gctgcagcac ggcggccggt
     2281 ggcgttccgg gacctgcggg catcttaggg atcgggcgac agcctgtctc gcgagtatcg
     2341 ggctgctatc ctcgcgaaaa ggacattccc cgtgacccgc cccaccgtcc cgaccgacca
     2401 gcgctacttc accgacgtcg accaggatcc gatgccggtg gtctccaagg cgaccgacta
     2461 ccccgccggc cacgtgatcc cgccgcacac ccacgtgcgc gggcagctcg tctactcgga
     2521 gcacggcgtg gtgatgctca ccgccgcggg cggctcgtgg atcgtgccgc cgacgcgcgc
     2581 cgtctggatc cccgccggca cgctgcacca cctgcgctgc atcggcgccg tgcgcatgcg
     2641 cagcctgtac ctgcggcccg acctcacgcc cgagctgccc gcccatgccc aggccgtctc
     2701 ggtctcgccg ctgctgcgcg agctcatcct cgccgccgtc gcggtggtgc cgccgtactc
     2761 gatggattcg cgcgacggcc gcgtgatgcg gctgctgctc gacgagctcc aggcgctgcc
     2821 cgtgctgccc ctgcacctgc cgcagcccgc cgacccgcgc ctgcgccgcg tctgcgagcg
     2881 cctcgccgcg cagcccgacg acacctcgac gctggccgac tgggcggccg agctggcggt
     2941 cgacgcgaag accatccagc gcctgttcca gcgcgaggcc gggatgacct tcggccagtg
     3001 gcgccagcag gcgcgcctgc tgcacgcgct cgagcggctc gcgctcggcg accgcgtgct
     3061 cgacgtggcg ctcgcgctcg gctacgaaag cccgagcgcc ttcgccacca tgttcaagcg
     3121 ccagttcggc gagacgccca gcgccttctt ccgctgatgt tgtcaggagt tctccacacg
     3181 gcttcgtgaa gtctcccgac tggtcggacg cggcgcggcg ccgaaaactc gaggagcaga
     3241 caacaacgga gtcctcatga agttctcgga cctgcgcgtc aagacaaagc tcatcctggg
     3301 gttctccgtg ctggccgccg tcgtggtgct ggtgtccgtc ctcgccctgc ggtcgctcgg
     3361 ccgcgccgac gaccgcctgt ccggctacct gtcgggcatc gcccagcgcg agcgcctggc
     3421 gcaggagatc cgcgccgccg ccgacgcccg ggccatcgcc gcgcgcaacc tggtgctggt
     3481 cacggagccg tccgacatcg ccctcgagaa ggccgccgtc accgcctcgc atgccgaggt
     3541 gggcgcggcg ctggcgcggc tgaaggcggc cgtggcggcg gcgccggacg cgaccgcgcg
     3601 cgagcgcgcg ctgcttgcgg acatcgagcg catcgagtcg cagtacggcc ccgtcgcgct
     3661 ggacatcgcc gccaaggcgc tcgacggccg caaggaggac gcgacgctcg cgatggacac
     3721 gcgctgccgc ccgctgctcg cgcagttgct caaggccacc agcgacgaca tcgcctacgc
     3781 ccgccaagtc gccgacgaac gcgtcgccgc ctcgcacgcc agctacgccg gcgaccgcgc
     3841 cgtgcaggcc accgtctcca cggcggccgt gctcctggcg ctggggctcg gctggctgct
     3901 ggcgcgcgcc gtcacgcgac cgctggagcg ggccgtcgcc atcgccgagg cggtcgccgg
     3961 cggcgacctc acctcgagcg tggtcgtcga ccgcaaggac gagacgggcc agctgctcgc
     4021 cgcgctggga cgcatgaacg aaggcctcgt gcgcatggtc gcccaggtgc gcgagtcggc
     4081 cgaaggcatc gcctcggcgt cctcgcagat cgccgccggc aaccaggacc tgtcctcccg
     4141 caccgagcac caggccagcg cgttgcagca gaccgccgcg tcgatgcacc agatgacttc
     4201 aaccgtgcag caggccgccg gccacgcccg caccgcgagc cagctggccg cggcggccgc
     4261 ggaagtcgcc ggccgcggcg ggcaggcggt gcaccgcgtg gtctcgacga tgggcgagat
     4321 cagcgcctcg agccagcgca tcgccgacat catcggcgtg atcgacggca tcgcgttcca
     4381 gaccaacatc ctcgcgctga acgcggcggt cgaggcggcg cgcgcgggcg agcagggccg
     4441 cgggttcgcg gtcgtcgccg gcgaggtgcg ctccctcgcg cagcgctccg ccgcggcggc
     4501 ccgggagatc aaggcgctga tcggcgccag cgcggagcgc gtgcaggccg gctcgtcgct
     4561 ggtgggcgaa gccggcgcga cgatggacga cgtcgtcgcg caggtgcgcc gcatgaccga
     4621 cctcatgggc gagatcggcg cgtcgtccca ggagcaggag cgcggcatcg tccaggtcaa
     4681 ccaggcggtc gcctcgatcg acgagggcac gcagcagaac gccgcgctcg tcgagcaggg
     4741 cgccgcggcc gccgagagcc tgaagcagca gtccgttcgg ctgctcggga tggtggcggc
     4801 gttccggctc gccgatcact gcgccgcggc tgcctgagcg gccgaacccc tccgacttcg
     4861 cggccggcac cgtccggccg accgcctttc ccgatccccg accccatgcg cgccatcgcc
     4921 tacgtcagca ccgcctcgtc gaatctcctg cgcgaggacc tggaggccat cgtcgccgcc
     4981 tcccgcggcc gcaacgccga gtccggcatc accggcgtcc tgctgtactg cgacggccac
     5041 ttcatgcagt acatcgaagg cccggacgcg gccgtgctcg aggccttcga gcgcatccgc
     5101 cgcgacgcgc gccactacca ggtcaacgag ctgctgaacc aggcgatcac cgagcgcgag
     5161 ttcgccgact ggacgctcgg cttcacccgg gcgtcgcctg cgaccgtcct cgacccgacc
     5221 acccgatcct gggatcgccc cgaccacgac gggccgggcg tagagatgct gcgtgtgttc
     5281 tggcgcaact gccgctccgt gacggcctga cccaccgccg ggcgcttgcg cgacgcgccc
     5341 cgggcaggac gcgcatcgcc cgcggctcgc cgcggcgtcc ggccggctgc ctgcctcacc
     5401 cgcgcgcgaa gtcggcgatc agcgcgttga cctgctgccg gtgcgtgacg tacagcccgt
     5461 gcggcgcgcc gtcgatctcg accagccgcg cgccggggat cagcgccgcc gcgggtcgcc
     5521 ccgtcagctc gaccggcgcc gacgcgtcgg ccgtgccgtg cacgatcagc acggggacgt
     5581 ccacccgcgc gagctcggga cggaagtccg tgtgcagctg caggcgcgcg agctccacgg
     5641 tcgcctgcat cgaggcgcgc aacatgtcgc cggccgtcca gtcgatcagc ggccggttgc
     5701 ccgcggcgcc gaagtagggc gccgagttcg cctcgatcca ggccgggaag tcgtcctcga
     5761 gcgccgccag gtgcgcgtcg atcgccgcct gcggcaggcc gcccgggttg tcggcggcct
     5821 ggcgcaggaa gggcacggcc gccggcgcga gcagcagcac gcgcc
//
LOCUS       DASVLN010000115        16188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_220453, whole genome shotgun sequence.
ACCESSION   DASVLN010000115 DASVLN010000000
VERSION     DASVLN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16188
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220453"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VF453_02475"
     CDS             <1..212
                     /locus_tag="VF453_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX7686539.1"
                     /translation="LGLVALSLEAALVAQAPSDPYYAESLQAWEQGRFIHFHGLAQWV
                     GWLWPFAAGVGLFRRVVAAGGVESP"
     gene            303..1574
                     /locus_tag="VF453_02480"
     CDS             303..1574
                     /locus_tag="VF453_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686540.1"
                     /translation="MGNGPLGGNTNPGISDGGTLARMLNLPPGTLSFSMPSWAEVENR
                     ARHAAHKLVNAVSTTDTSTAIGQLLGQQSFTAGIVYGMCANLAGSVWQLAKLFKTLAL
                     AEYWDTKHAGTFWERAAHSFSFGPMGPSMMDGLSAVASFWPGLDDQARRAHDDREALK
                     QLVIYSFEHPGEVFGKIGATEKRKYEEFKAFLARHTMSGDFHAGALFGELLFDILLLI
                     DGVTAIAKIAAKIPGLLRLLPELEELAPALRAALRKPEVPKAGEFAEGPKPQAVEAKP
                     SAAGDAAADAAPAKPDPTKMTTSEKGVYGEAMSDQYMDGKGFEKMNGDLVQVGDKPLG
                     QGIDGVWKNGSPPPEYVITESKYGSSQLSTLKDGTKQMSDKWVSDRLDNAVGEEAADD
                     IRYASSRGQVQKWLLQVDSNGNVTKSIIPPG"
     gene            1591..2316
                     /locus_tag="VF453_02485"
     CDS             1591..2316
                     /locus_tag="VF453_02485"
                     /inference="COORDINATES: protein motif:HMM:NF020501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PoNe immunity protein domain-containing protein"
                     /protein_id="HEX7686541.1"
                     /translation="MGKREPLLTEEFFVNRLAYLDRIVAERNERLANEPEVYTHPKEL
                     LWSNFRGRYQQIFLGYSLDEDVPRLAQRFPAVVETYEIYLRDKEAVPTDLADLDEYVV
                     SLWLVSFAILFKVDDDLWKRLLACIGNEGRDALFEALVATRSPNRKKATGLLHAAIFQ
                     PLYDAIAADAKSRDALVKRYLKAWYKALKPTYWVDSHKGPKGGGFFGYWAVEVAGVVQ
                     AFGMDDAGWRDMPYYPADLVTRA"
     gene            2349..3074
                     /locus_tag="VF453_02490"
     CDS             2349..3074
                     /locus_tag="VF453_02490"
                     /inference="COORDINATES: protein motif:HMM:NF020501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PoNe immunity protein domain-containing protein"
                     /protein_id="HEX7686542.1"
                     /translation="MSKREPLMNEAFFLDGLAYADRIIESRNTKLREQPEVYKFPKEF
                     LWSNFRDRYQQIFLGYSLEEDVPRLAQRFPAVVETYEIYLRDKAAVPTDLADLDEYVV
                     SLWLVSFAILFKVDDDLWTRLLACIGNEGRDALFEALVATRSPNRKKAASLLHAAIFQ
                     PLYDAIAADAKSRDALVKRYLKAWYKALKPTYWVDSHKGPKGGGFLGYWAVEVAGVTQ
                     AFGMDDAGWRDMPYYPEDLVTRA"
     gene            3135..3497
                     /locus_tag="VF453_02495"
     CDS             3135..3497
                     /locus_tag="VF453_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX7686543.1"
                     /translation="MTARSGAVPVTQPPYYKHHVFFCLNERPAGEDSCARVGAQAGFE
                     RCKSRVKKEGLAGPGGVRVNKAGCLDRCAGGPVAVVYPEGTWYTYVDEQDIDEIVDSH
                     LKGGRPVERLLLPPEIGR"
     gene            3516..4142
                     /locus_tag="VF453_02500"
     CDS             3516..4142
                     /locus_tag="VF453_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7686544.1"
                     /translation="MNAFTKRGEIAGPAGLLSVAVDTTTDLPSRGLAVFCHPHPLQGG
                     TMDNKVVTTVARGLMQRGWRVVRFNYRGVGASQGTWDEGRGEVDDALAVIAAHRAAGE
                     PLVLGGFSFGGYIALSAAMKLPEAERPRRLVLVAPSTVKHEAPPATAAAADTLLIHGE
                     ADDVVPLAASLDWARPQAVPVVVVPGAGHFFHGQLPLLKSLVLRHFAE"
     gene            4159..5319
                     /locus_tag="VF453_02505"
     CDS             4159..5319
                     /locus_tag="VF453_02505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7686545.1"
                     /translation="MKTLLRAAGLACAALIAPLQAGAQALPPPEIAGRAYLLVDMNAD
                     QVLAEHEADAPADPASLTKLMTAYIVFVALHDHKLALDQRLPVSQRAWAERKGGGSLM
                     FIDPKMTPTVDELLQGLIVDSGNDAAVALAEGVAGSTDAFVGMMNRQAQAWGLKNTTF
                     RNVTGLTEAGHRSTARDMVAMAEHIVRDYPEYYHYYAQRDYTFNNIRQDNRNLLLERD
                     PSVDGMKTGYTEAAGWCLLASAQRDFPNGKRRLMAIVMGADSMQGRANETQKLLNWGF
                     TAFDDVRLVEAGKAVATVPVWKGRQAQVGIGSAQAIYVAVPKGEGDRLKTQVERTDPL
                     VAPLASGQRVGQLKVATASGTAIAAIPLTVLEPVPEAGLFGRAWDALRLWIK"
     gene            5384..6274
                     /locus_tag="VF453_02510"
     CDS             5384..6274
                     /locus_tag="VF453_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX7686546.1"
                     /translation="MASLPDTLCYLNGDYLPLNQAKVSVMDRGFVFGDGAYEVIPVYD
                     RRMFRFDEHLARLARSLGKLDIEIDLERETWLGRLRKLIAAFPSGDQLLYIQVTRGVA
                     PRSLVAPPGLEPTVFASTSERVALTPAQRAQGLSCVTARDFRWQRGDIKSTSLLGSVL
                     ARRVSADQGADETLLLRDDEPAGPWLTEGASSNAWIVQEGALLGPTPGPRVLEGVRVE
                     LLRELCEECAIAFNLRPVSEADLRAADEVLLSSAGKEIVSVTRLDGEAVGHGALRGRP
                     GPVYARLHEAYQRAIESQSL"
     gene            6294..6569
                     /locus_tag="VF453_02515"
     CDS             6294..6569
                     /locus_tag="VF453_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HEX7686547.1"
                     /translation="MHEIPPEQSLIEYPSAFPIKVMGPVAEGFVEAIVEVARQFDPEF
                     DEATVEIRPSKGGNYLGITITVTATSRPQLDELYRTLTTHPMVKIVL"
     gene            6607..7092
                     /locus_tag="VF453_02520"
     CDS             6607..7092
                     /locus_tag="VF453_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686548.1"
                     /translation="MHLTRNETEKCVMARIVRQKRLHQKNFTLRQFGTWTKAEAAARK
                     WVKATLAELPPPINMKNRMTSRNTSGVVGVRLVHAVRRREDKEYPDWRWIAFWPGCPN
                     SGGIGWSVNKYGDERAFVNACLARKLESVDRAEIDEEYDRFRTTRDYKRLLRLKQITP
                     G"
     gene            7169..7891
                     /gene="lipB"
                     /locus_tag="VF453_02525"
     CDS             7169..7891
                     /gene="lipB"
                     /locus_tag="VF453_02525"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX7686549.1"
                     /translation="MPSQPVSDEAPSTAIAAAPVVRLLGRVPYEATVEAMKAFTAARD
                     ESTADEIWIVEHEPVFTQGLAGKPEHLLAPGDIPVVQTNRGGQVTFHGPGQVVGYPLV
                     DLRRLGIYVKEYVFRLEEALLRTLAAQGVTGHRVAGAPGIYVRMADPFGHAALTGPRP
                     GGDPFRGLGKVAALGVKVSNHRTYHGVALNVAMDLSPFARINPCGYAGLQTVDLATLG
                     VFADWTTVAEDLGRRLAAQFTC"
     gene            7965..8999
                     /gene="lipA"
                     /locus_tag="VF453_02530"
     CDS             7965..8999
                     /gene="lipA"
                     /locus_tag="VF453_02530"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX7686550.1"
                     /translation="MSTEPNAPVAASDAAAPAYDASAKQKAQAKTSRIPIKIVQAEQL
                     KKPDWIRVRAATPSSRFYEIKQILREHKLHTVCEEASCPNIGECFGKGTATFMIMGDK
                     CTRRCPFCDVGHGRPDPLDPEEPLNLARTIAALKLKYVVITSVDRDDLRDGGAGHFVE
                     CIRQVRALSPETRIEILVPDFRGRDERALEILKAAPPDVMNHNLETAPRLYKQARPGS
                     DYQFSLNLLKKFKALHPDVPTKSGIMVGLGETDEEILQVMRDMRAHDIDMITIGQYLA
                     PSGHHLPVMRYVHPDTFRMFEEKAVEMGFTHAAVGAMVRSSYHADKQAEQAGVPIDVP
                     RTAKASDPVA"
     gene            complement(9000..9491)
                     /locus_tag="VF453_02535"
     CDS             complement(9000..9491)
                     /locus_tag="VF453_02535"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7686551.1"
                     /translation="MKTARTAARIAAALAFTMLAGCDHAPQAPDVGYTLLDGRAQHLA
                     QLRGHVVLVNFWASDCVPCTRELPRLAQSWRRFAPKGFDTLAVSMRYDPPAQAIAFAR
                     SRALPFGVVIDLTGEVAAAFGHVDATPTTVLIDRQGREVARWIGPTDFDQLDRRLAQL
                     LDG"
     gene            9504..10208
                     /locus_tag="VF453_02540"
     CDS             9504..10208
                     /locus_tag="VF453_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HEX7686552.1"
                     /translation="MRRREFNVASVAILVARAFLPGGFLVAAGEALALGESEAAAGVR
                     EALERGANGAVDLLGRPGGFLDNPKVRIPLPGALDTAATLLRNLGQGRRVDELVVAMN
                     RAAEAAVPEARPLLVNAVKTMSVEDAVRIVRGGDTSVTDFFASKTRPALTSRFLPIVT
                     RETQKVSLAEKYNAVASKGSAFGLVRPEDANVQQYVTRKALDGLFLMIGEEERRIRAD
                     PVGTGSALLKAVFGGR"
     gene            complement(10220..11368)
                     /locus_tag="VF453_02545"
     CDS             complement(10220..11368)
                     /locus_tag="VF453_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686553.1"
                     /translation="MAMTMAANYPIMTDPVQQAQRPEADEAHWREIMSQIGSEVGLPL
                     SSALERVTALATTGKIDRAGLRQLREEIERARRAGMIGQQLARFASGRIRQTPEQVSV
                     TQMLREVLVQRGREAAARQIELRQALRPAEVVADATLLYALLQAVVDWGLDLAKSSIE
                     FKLDIKAWPPYARLTCRFAHAGRDGADPRHAAAPQPALDSMAWRLVQQISWTLSLVMD
                     RVVSEHETTLTLEFPRTINEQIEGVSVVEIDQGFGVSDNSKPLAGSHVLVIAGNRDVR
                     TQIRDSLRHMGLLVDFVNTVEEAEEFCRDALPHAIVFESVLVTERFQKLRASLVAEVA
                     NYVFIEIAAEGSSMQLSSPAEGQRAVIGRDAIAQSLPSALIYELSRTS"
     gene            11367..12104
                     /locus_tag="VF453_02550"
     CDS             11367..12104
                     /locus_tag="VF453_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686554.1"
                     /translation="MIPPSSPCRPRAAGAIVRRIGQSLQIVSKPDRRWAILQSVTRFF
                     HPQRGGVAAQATILRLGKTRFWLRAAGAAAVAAGLAGCSPALDWRDVRLPDTALVTQL
                     PCKPGRFGRKVEVAGRPLQLFMLSCEAGGVTYGVATAEIGDPAQVPAVLDGLASAAVS
                     ALHAAEPRFAPFAMEGVTPFHENVSARLHGVRPDGQAVEESIRVFARGTRVFQASAIG
                     AALPDPAVQPFHEGLRFDLERKDAGPS"
     gene            12263..12712
                     /locus_tag="VF453_02555"
     CDS             12263..12712
                     /locus_tag="VF453_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEX7686555.1"
                     /translation="MAAVLLIAHAPLASALRGVAGHCFAEKGHLFEAVDVDPTMSADD
                     AEALARAAVARLASPEVLILTDVFGATPCNAAQRLADGAAVRVVAGVNVPMLWRSLCY
                     LAEPLDALVARAVSGATQGVLQVAQTRPQNQNPSISSNDPDHARHQQ"
     gene            12681..12950
                     /locus_tag="VF453_02560"
     CDS             12681..12950
                     /locus_tag="VF453_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX7686556.1"
                     /translation="MIRTTLVISNKLGLHARASAKLTKLATSFKSEVFMSRNGRRVNA
                     KSIMGIMMLAAGLGSEVELETEGPDEQEASDAIRALVDDKFGEGQ"
     gene            complement(13009..13839)
                     /locus_tag="VF453_02565"
     CDS             complement(13009..13839)
                     /locus_tag="VF453_02565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX7686557.1"
                     /translation="MSELRNAIGAATLDGLRRPERAAGRTQRSHVAPQLHAVWATHLD
                     DVRAAQRLRHQVFVEEMGARPTPLVGAPAGHDIDVFDEFCEHLIVRTTPAADEPGQVV
                     GTYRLLTPAAARRAGSFYSDTEFDLTRLRPLRPRMVELGRSCVHPDHRSGGVILALWG
                     ALAQFMERNGMETVIGCASLSMRDGGHCAASLWAKLRETHLAGLEWQVRPRVPLPVGD
                     LEQGLDVEPPALIKGYLRLGAKVLGAPAWDPEFGTADLPMLTRVADMPERYRRHFLAA
                     "
     gene            14033..15811
                     /gene="ptsP"
                     /locus_tag="VF453_02570"
     CDS             14033..15811
                     /gene="ptsP"
                     /locus_tag="VF453_02570"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX7686558.1"
                     /translation="MSIQVFGLPVSRGVAIGRAVLVASSRVDVAHYFIEPALIEAEVL
                     RLRAARDLVVEELTALKEDVPEDAHDELVALLDVHLMLLQDETLADATRQWIVERHYN
                     AEWALSSQLEVIARQFDEMEDEYLRDRKGDLEQVVERVIAALGRLAGDSFAEPPPHSP
                     RDFAGEDPLVLVANDIAPADMLRFRRSVFTGFVTDVGGRNSHTAIVARSMDIPAVVGA
                     REASRIVTQDDWIIIDGDAGVVIVNPSPIVLEEYRFRQRQVELERARLDRLRNTPAVT
                     MDGQAIELLANIELPSDVQAVLDAGAVGVGLFRSEFLFMNRNGRLPGEEEQYQAYRRV
                     VEGLDGLPVTIRTIDIGADKPLDRDQDGRDRDHALNPALGLRAIRWSLSEPEMFGAQL
                     RAILRASALGKVRLLVPMVAHLREARLVLEAIERAKAQLRARAQAYVDVQVGAMIEVP
                     AAAILLPSLLKYFDFISIGTNDLIQYTLAIDRADESVSHLYDPWHPAVLQLLSQTIAK
                     ANELGKEVCVCGEMAGDPAFTELLLGMGLRSFSMHPTQVSSIKKRILRVDTKAWSERI
                     DLIVNADDPQAICARLHTAAPAPDGA"
BASE COUNT         2542 a         5612 c         5656 g         2378 t
ORIGIN      
        1 cgctggggct ggtcgcgctg tcgctggagg cggcgctcgt ggcgcaggcc ccgtccgatc
       61 cctactacgc cgagagcctc caggcgtggg agcagggccg cttcatccac ttccacggcc
      121 ttgcgcagtg ggtcggctgg ctgtggccct tcgcggccgg ggtcgggctg ttccggcgcg
      181 tggtcgccgc gggcggcgtc gaaagcccct gacgcgcagc tctgcaatgg agttgtcgcg
      241 cgggctgtgt cagagtcgcg ccgtggcgcg ttgcgcgccg caccggcaca cagggagtgc
      301 gcatgggcaa tggacctctg ggcggcaaca cgaaccccgg catctccgat ggcggcacgc
      361 tggcgcggat gctgaacctg ccgccgggca cgctgtcgtt ctcgatgccc agctgggccg
      421 aggtcgagaa ccgcgcgcgc cacgccgcgc acaagctggt caacgcggtc agcaccacgg
      481 acacctcgac cgcgatcggc cagctcctgg gccagcagtc gttcaccgcg ggcatcgtct
      541 acgggatgtg cgccaacctg gcgggcagcg tgtggcagct ggccaagctg ttcaagacgc
      601 tcgcgctcgc cgagtactgg gacaccaagc acgccggcac cttctgggag cgcgcggcgc
      661 actcgttcag cttcgggccg atggggccgt cgatgatgga cgggctgtcg gccgtcgctt
      721 cgttctggcc cggcctggac gaccaggccc gccgcgccca tgacgaccgc gaggcgctca
      781 agcagctggt catctactcg ttcgagcatc cgggcgaggt gttcggcaag atcggcgcga
      841 ccgagaagcg caagtacgag gagttcaagg ccttcctcgc gcgccacacg atgtccggcg
      901 acttccatgc cggcgcgctg ttcggcgagc tgctgttcga catcctgctg ctgatcgacg
      961 gcgtcaccgc gatcgccaag atcgccgcca agatccccgg cctgttgcgg ctgctgccgg
     1021 agctcgagga actcgcgccc gcgctgcgcg ccgccctgcg caagcccgag gtgcccaagg
     1081 cgggcgagtt cgccgagggc ccgaagccgc aggcggtcga ggccaagccc tccgccgccg
     1141 gcgacgccgc agccgacgcc gcgcccgcca agcccgaccc gaccaagatg acgacgtccg
     1201 agaagggcgt ctatggcgag gcgatgtccg accagtacat ggacggcaag ggcttcgaga
     1261 agatgaacgg cgacctggtg caggtgggcg acaagccgct cggccagggc atcgacggcg
     1321 tctggaagaa cggctcgccg ccgccggagt acgtcatcac ggagtcgaag tacggctcgt
     1381 cgcagttgtc cacgctcaag gacgggacga agcagatgag cgacaagtgg gtgtccgatc
     1441 gcctggacaa cgcggtcggc gaagaggctg cggacgacat ccgctacgcc agttcccgcg
     1501 gccaggtcca gaaatggttg ctccaggtcg atagcaacgg caacgttacc aagagcataa
     1561 tcccgcccgg ttaaaagcga ggatccacga atgggaaagc gcgaacccct gctgaccgaa
     1621 gagttcttcg tcaatcgtct ggcctatctc gatcgtatcg tcgccgagcg caacgagagg
     1681 ctggccaacg agccggaggt gtatacgcac ccgaaggagc tcctctggtc gaatttccgc
     1741 gggcgctatc agcagatctt cctgggctac agcctcgacg aggacgtgcc gcgcctggcg
     1801 cagcgcttcc ccgcggtcgt cgagacctac gagatctacc tgcgcgacaa ggaggccgtg
     1861 ccgaccgacc tggccgacct cgacgagtac gtcgtctcgc tgtggctggt gagtttcgcc
     1921 atcctgttca aagtcgacga cgacctgtgg aagcgcctgc tggcctgcat cggcaacgag
     1981 ggccgcgacg ccctcttcga ggcgctggtc gcgacgcgtt cgccgaaccg caagaaggcg
     2041 acgggcctgc tgcacgcggc gatcttccag ccgctgtacg acgcgatcgc cgcggacgcg
     2101 aagtcccgcg acgcgctggt caagcgctac ctgaaggcct ggtacaaggc gctcaagccg
     2161 acctactggg tcgactcgca caaaggtccg aagggcggcg gcttcttcgg ctactgggcc
     2221 gtcgaggtcg cgggcgtcgt gcaggcgttc ggcatggacg acgccggctg gcgcgacatg
     2281 ccgtactacc cggccgacct cgtgacccgg gcctgattcc aaaatcggca gcatgcgaga
     2341 ctgcccggat gagcaagcgc gagccattga tgaacgaggc cttctttctg gacggcctgg
     2401 cttacgccga caggatcatc gaatcgcgca acaccaagtt gcgcgagcag cccgaggtct
     2461 acaagttccc gaaggagttc ctatggtcga acttccgaga tcgctaccag cagatcttcc
     2521 tgggctacag ccttgaagaa gacgtgccgc ggttggcaca gcgcttcccg gcagtcgtcg
     2581 aaacctatga gatctacctg cgcgacaagg ccgcggtgcc gaccgacctg gccgacctcg
     2641 acgagtacgt cgtctcgctg tggctggtga gtttcgccat cctgttcaaa gtcgacgacg
     2701 acctgtggac gcgcctgctg gcctgcatcg gcaacgaggg ccgcgacgcg ctgttcgagg
     2761 cgctggtggc cacgcgctcg ccgaaccgca agaaggcggc gagcctgctg cacgcggcga
     2821 tcttccagcc gctgtacgac gcgatcgccg cggacgcgaa gtcgcgcgac gcgctggtca
     2881 agcgctacct gaaggcctgg tacaaggcgc tcaagcccac gtactgggtc gactcgcaca
     2941 agggtccgaa gggcggcggc ttcctcggct actgggccgt ggaggtcgcg ggcgtcacgc
     3001 aggccttcgg catggacgac gccggctggc gcgacatgcc ctactacccg gaagacctgg
     3061 tcacccgggc ctgaacgccg ggccggtgcg ctaagaaccc ttgtcggaca ggcgccgcgg
     3121 cgcggcaagg taggatgacg gcccgctccg gagccgttcc cgtgacccag ccgccgtact
     3181 acaagcacca cgtcttcttc tgcctcaacg agcgtcccgc cggcgaggac agctgcgccc
     3241 gcgtcggcgc gcaggcgggc ttcgagcgct gcaagtcgcg cgtgaagaag gagggcctcg
     3301 ccggcccggg cggcgtgcgc gtgaacaagg ccggctgcct cgaccgctgc gccggcggcc
     3361 cggtcgccgt cgtctatccc gaaggcacct ggtacaccta cgtcgacgag caggacatcg
     3421 acgagatcgt cgactcccac ctcaagggcg gccggcccgt cgagcgcctg ctgctgccgc
     3481 cggaaatcgg ccgctgacgc gagcgccgtc ccgtcatgaa cgccttcacg aagcgcggcg
     3541 agatcgccgg tcccgccggg ctcctgagcg tcgccgtcga caccacgacc gatctgccct
     3601 cgcgcggcct ggccgtgttc tgccatcccc acccgctgca gggcggaacg atggacaaca
     3661 aggtcgtcac gaccgtcgcc cgcggcctga tgcagcgcgg ctggcgggtg gtgcgcttca
     3721 actatcgcgg cgtcggcgcc tcgcagggca cctgggacga gggccgcggc gaggtcgacg
     3781 atgcgctcgc ggtgatcgcg gcccaccgcg ccgcgggcga gccgctggtg ctgggcggct
     3841 tctcgttcgg cggctacatc gccttgagcg cggcgatgaa acttccggag gccgagcggc
     3901 ctcggagact ggtgctcgtc gccccgtcga ccgtgaagca cgaggcgccg ccggccaccg
     3961 ccgcggccgc cgacacgctg ctgatccacg gcgaggccga cgacgtcgtg ccgctcgcgg
     4021 cctcgctcga ctgggcgcgg ccgcaggcgg tgcccgtcgt cgtcgtcccc ggcgccgggc
     4081 atttcttcca tggccagctg cccctgctga agtccctggt gctccgacat ttcgccgagt
     4141 gacgccgtca cgcctccgat gaagacactg ctccgcgccg cgggcctggc ctgcgccgca
     4201 ttgatcgctc ccctgcaggc cggcgcgcag gcgctgccgc cccccgagat cgccggccgc
     4261 gcctacctgc tggtggacat gaacgccgac caggtgctcg ccgagcacga ggccgacgcg
     4321 cccgccgatc cggcgtcgct gaccaagctg atgacggcct acatcgtgtt cgtcgcgctg
     4381 cacgaccaca agctggcgct ggaccagcgg ctgcccgtct cgcagcgcgc ctgggccgag
     4441 cgcaagggcg gcggatcgct gatgttcatc gacccgaaga tgacgccgac ggtcgacgag
     4501 ctgctgcagg gcctgatcgt cgactcgggc aacgatgccg cggtggcgct ggccgagggc
     4561 gtggcgggca gtaccgacgc cttcgtcggg atgatgaacc gccaggcgca ggcctggggc
     4621 ctgaagaaca cgaccttccg caacgtcacc ggcctgaccg aggcgggcca ccgcagcacg
     4681 gcgcgggaca tggtcgcgat ggcggagcac atcgtccgcg actaccccga gtactaccac
     4741 tactacgcgc agcgagacta cacgttcaac aacatccgcc aggacaaccg caacctgctg
     4801 ctggagcgcg acccgagcgt ggacggcatg aagacgggct acaccgaggc cgccggctgg
     4861 tgcctgctcg cgagcgcgca gcgcgacttc ccgaacggca agcgccgcct gatggccatc
     4921 gtgatgggcg cggactcgat gcaaggccgc gccaacgaga cccagaagct cctcaactgg
     4981 ggcttcacgg cgttcgacga tgtgcgcctc gtcgaggccg gcaaggcggt ggcgacggtg
     5041 ccggtgtgga agggccggca ggcgcaggtg ggcatcggca gcgcgcaggc gatctacgtc
     5101 gccgtgccca agggcgaggg cgaccgcctg aagacgcagg tcgagcgcac cgacccgctc
     5161 gtcgcgccgc tcgcctcggg ccagcgtgtc ggccagctga aggtggcgac ggcttccggc
     5221 accgcgattg ccgcgatccc cctcaccgtc ctcgagccgg tgcccgaggc gggcctgttc
     5281 ggccgcgcct gggatgcgct gaggctctgg atcaagtaac ctgtccgcca cgcgcccctc
     5341 cccgccgccg ggtggtgcgg gcgcccgtcc cggaggatcg accatggctt ccttgcccga
     5401 caccctgtgc tacctcaatg gagactacct tccgttgaac caggccaagg tctcggtcat
     5461 ggaccgcggc ttcgtgttcg gcgacggcgc ctacgaggtg atcccggtct acgacaggcg
     5521 catgttccgc ttcgacgagc acctcgcgcg gctcgcgcgc agcctcggca agctggacat
     5581 cgagatcgac ctggagcgcg agacctggct cggccgcctg cgcaagctga tcgcggcatt
     5641 cccgtccggc gaccagctgc tctacatcca ggtcacgcgc ggcgtggcgc cgcgctcgct
     5701 cgtcgcgccg ccgggcctcg agccgacggt cttcgcttcc accagcgagc gcgtcgcgct
     5761 gacgcccgcg cagcgcgcgc agggcctgtc gtgcgtgacg gcgcgcgact tccgctggca
     5821 gcgcggcgac atcaagagca cgtcgctcct gggcagcgtg ctcgcgcgcc gcgtctcggc
     5881 cgaccagggc gccgacgaga ccctgctgct gcgcgacgac gagcccgcgg gcccgtggct
     5941 caccgagggc gcttccagca acgcgtggat cgtccaggag ggggcgctgc tcggccccac
     6001 gccggggccg cgcgtgctcg aaggcgtgcg cgtcgagctc ctgcgcgagc tctgcgagga
     6061 atgcgccatc gcgttcaacc tgcgcccggt cagcgaagcc gacctgcgcg ccgccgacga
     6121 ggtgctgctc agctccgccg gcaaggaaat cgtctcggtc acgcgcctgg acggcgaggc
     6181 ggtcggccac ggcgcgttgc gcggccggcc gggtccggtt tatgcccggc tccacgaggc
     6241 ctaccaacgc gcgatcgaat cccagtccct ttgatagacc cacggtccgc accatgcacg
     6301 agatcccgcc cgagcagtcg ctcatcgaat accccagcgc gttccccatc aaggtgatgg
     6361 gcccggtcgc ggagggcttc gtcgaggcga tcgtggaggt cgcaaggcag ttcgacccgg
     6421 aattcgacga agcgacggtg gagatccggc ccagcaaggg gggcaattac ctgggcatca
     6481 cgatcacggt cacggccacc agccgcccgc agctggacga gttgtaccgg acattgacca
     6541 cgcatccgat ggtcaagatc gttctgtgat gcagggagac ggaacgcgaa tctctcgaga
     6601 ctttgcatgc atctcacccg caacgagacc gagaaatgcg tcatggcgcg catcgtccgc
     6661 cagaagcggc tgcatcagaa gaacttcacg ctgcggcagt tcggcacctg gacgaaggcc
     6721 gaggcggccg ctcgcaagtg ggtgaaggcg acgctggccg agctgccgcc gccgatcaac
     6781 atgaagaacc gcatgacctc gcgcaacacc tcgggggtcg tgggcgtgcg gctcgtgcac
     6841 gcggtgcggc ggcgcgagga caaggagtac ccggactggc gctggatcgc gttctggccg
     6901 ggctgcccga actcgggcgg gatcggctgg tcggtcaaca agtacggcga cgagcgggcg
     6961 ttcgtcaacg cgtgcctcgc gcgcaagctg gagagcgtcg accgcgcgga gatcgacgag
     7021 gagtacgacc gcttccgcac gacgcgcgac tacaagcggc tgctcaggct caagcagatc
     7081 acgcccggct gaccccgcgc cggatgacgc cgcggcgcgc gccgtaagat gccggcgcgg
     7141 cgtttcgccg tgccccgagc cgccccgcat gccttcccag ccggtttccg acgaggcccc
     7201 gtccaccgcc atcgccgccg cgccggtcgt ccgccttctg ggccgcgtcc cctacgaggc
     7261 gaccgtcgag gccatgaagg cgttcacggc cgcgcgcgac gaaagcacgg ccgacgagat
     7321 ctggatcgtc gagcacgaac ccgtcttcac ccagggcctg gccggcaagc ccgagcacct
     7381 gctcgcgccc ggcgacatcc ccgtcgtgca gaccaaccgg gggggacagg tgaccttcca
     7441 cgggccgggg caggtcgtcg gctacccgct ggtcgacctg cggcggctgg gcatctacgt
     7501 caaggaatat gtcttccgcc tcgaggaggc gctgctgcgc acgctcgccg cgcagggcgt
     7561 gaccgggcac cgcgtggccg gcgcgccggg gatctacgtg cgcatggccg acccgttcgg
     7621 ccatgccgcg ctcaccggtc cgcggccggg cggcgatccg ttccgcggcc tgggcaaggt
     7681 cgcggccctg ggcgtcaagg tgagcaacca ccgcacgtac cacggcgtcg ccctcaacgt
     7741 ggcgatggat ctgagcccct tcgcgcggat caacccgtgc ggttatgccg ggctgcagac
     7801 ggtcgatctc gctacactag gggttttcgc tgactggacg accgtggccg aggatctcgg
     7861 ccggcgtctt gcggcgcagt tcacctgctg acccgggcgg gcggcggcgc acgggtcggc
     7921 gccatccgcg cggctcgccg acaagccgcc ccgccaggag cccgatgtcc accgaaccga
     7981 acgcccccgt cgccgcttcc gatgccgccg cgcccgccta cgacgcgagc gccaagcaga
     8041 aggcccaggc caagacctcg cgcatcccga tcaagatcgt ccaggccgag cagctgaaga
     8101 agccggactg gatccgcgtg cgcgccgcga cgccgtcgag ccgtttctac gagatcaagc
     8161 agatcctgcg cgagcacaag ctgcacacgg tctgcgagga ggcgtcctgc cccaacatcg
     8221 gcgagtgctt cggcaagggc acggccacgt tcatgatcat gggcgacaag tgcacgcggc
     8281 gctgcccgtt ctgcgacgtc ggccacggcc gcccggatcc gctcgatccc gaggagccgc
     8341 tgaacctggc gcgcacgatc gcggcgctga agctgaagta cgtcgtcatc accagcgtcg
     8401 accgcgacga cttgcgcgac ggcggcgccg gccacttcgt cgaatgcatc cgccaggtgc
     8461 gcgcgctctc gcccgagacg cgcatcgaga tcctcgtgcc cgacttccgc ggccgcgacg
     8521 agcgcgcgct ggagatcctg aaggccgcgc cgcccgacgt gatgaaccac aacctcgaga
     8581 ccgcgccgcg gctctacaag caggcgcgcc cgggcagcga ctaccagttc agcctcaacc
     8641 tgctgaagaa gttcaaggcg ctgcacccgg acgtcccgac caagagcggc atcatggtcg
     8701 gcctcggcga gaccgacgag gagatcctgc aggtcatgcg cgacatgcgc gcgcacgaca
     8761 tcgacatgat cacgatcggc cagtaccttg cgccgtccgg ccaccacctg ccggtgatgc
     8821 gctacgtgca cccggacacg ttccgcatgt tcgaggagaa ggccgtcgag atgggcttca
     8881 cccatgccgc ggtcggggcg atggtgcgtt cgagctacca cgccgacaag caggccgagc
     8941 aggccggcgt gccgatcgac gtgccgcgca cggcgaaggc gtccgacccg gtggcctgat
     9001 cagccgtcca ggagttgcgc cagccggcgg tcgagctggt cgaagtccgt cggcccgatc
     9061 cagcgcgcga cttcgcggcc ctgccggtcg atcagcaccg tcgtcggggt cgcgtcgaca
     9121 tggccgaagg cggcggcgac ctccccggtg aggtcgatca ccacgccgaa cggcagcgcc
     9181 cgcgagcgcg cgaaggcgat ggcctgggcg ggcgggtcgt agcgcatgga gacggccagc
     9241 gtgtcgaaac ccttcggtgc gaagcggcgc caggactgtg cgagccgcgg cagctcgcgc
     9301 gtgcagggga cgcagtcgct cgcccagaaa ttcacgagga cgacgtgccc ccgcagctgc
     9361 gcgaggtgct gcgcgcggcc atctagcagc gtgtagccga cgtcgggtgc ttgcggcgca
     9421 tggtcgcagc ccgctagcat ggtgaatgcc agcgccgcgg cgatcctggc ggcggtgcgg
     9481 gccgttttca tgggggacga tcgatgagac gtcgcgagtt taacgtcgcc tccgtggcga
     9541 tcctggtggc gcgtgcgttc ctgcccggcg gcttcctggt cgcggcgggc gaggcgctcg
     9601 cgctcggcga atccgaggcc gccgcgggcg tgcgcgaggc gctggagcgc ggcgcgaacg
     9661 gtgccgtcga cctgctcggc aggcccggcg gcttcctcga caacccgaag gtgcgcatcc
     9721 cgctgcccgg cgcgctggac acggccgcca cgctgctgcg caacctcggc caggggcggc
     9781 gcgtcgacga gctggtcgtg gccatgaacc gcgctgccga agccgccgtg cccgaggcgc
     9841 ggccgctgct cgtcaacgcg gtcaagacga tgtcggtgga ggacgcggtg cgcatcgtgc
     9901 gcggcggcga cacctcggtg accgacttct tcgccagcaa gacgcgcccc gcgctgacct
     9961 cgcgcttcct gccgatcgtc acgcgcgaga cgcagaaggt ctcgctcgcc gagaagtaca
    10021 acgcggtggc gtccaagggc tcggcgttcg gactcgtccg gcccgaggac gccaacgtgc
    10081 agcagtacgt gacgcgcaag gcgctcgacg gcctcttcct gatgatcggc gaggaggaaa
    10141 ggcgcatccg cgccgacccg gtgggcaccg gcagcgcgct gctgaaggcc gtcttcggcg
    10201 ggcgctgagc ccaacggcgt cagctggtgc gcgagagctc gtagatcagc gccgacggca
    10261 gcgactgcgc gatcgcgtcg cgcccgatga cggcgcgctg gccctcggcc gggctggaca
    10321 gctgcatcga gctgccttcc gcggcgatct cgatgaagac gtagttggcg acctcggcga
    10381 cgaggctggc gcgcagcttc tggaagcgct ccgtgaccag caccgactcg aagacgatcg
    10441 cgtgcggcag ggcgtcgcgg cagaactcct cggcttcctc gacggtgttg acgaagtcga
    10501 ccagcaggcc catgtggcgc aacgagtcgc ggatctgcgt acgcacgtcg cggttgcccg
    10561 cgatcacgag gacgtggctg ccggcgagcg gcttggagtt gtccgagacg ccgaagccct
    10621 ggtcgatctc gacgacgctc acgccctcga tctgctcgtt gatcgtgcgc gggaactcga
    10681 gggtcagggt cgtttcgtgc tcggagacga cgcgatccat gacgaggctc agcgtccacg
    10741 agatctgctg caccaggcgc caggccatgg aatcgagcgc gggctgcggc gcggcggcgt
    10801 ggcgcgggtc ggcgccatcg cgcccggcgt gggcgaagcg gcaggtcagg cgcgcatagg
    10861 gcggccaggc cttgatgtcc agcttgaact cgatgctcga cttcgccagg tcgaggcccc
    10921 agtcgacgac ggcctgcagc agcgcataca gcaaggtcgc gtcggcgacc acctcggccg
    10981 gccgcagcgc ctggcgcagc tcgatctggc gcgcggcggc ctcgcgaccg cgctgcacga
    11041 gcacctcgcg cagcatctgc gtcacgctca cctgctcggg cgtctggcgg atgcgacccg
    11101 acgcgaagcg ggcgagctgc tggccgatca tgccggcccg gcgcgctcgc tcgatctcct
    11161 cgcgcagctg ccgcagcccg gcgcggtcga tcttgccggt cgtcgccagc gcggtgacgc
    11221 gctccagcgc gctcgacagg ggcaggccca cctcgctgcc gatctggctc atgatctcgc
    11281 gccagtgcgc ctcgtcggcc tccggtcgct gtgcctgctg gaccgggtcg gtcatgatcg
    11341 ggtagttcgc tgccatcgtc atcgccatga ttcctccctc ctcgccgtgc aggccgaggg
    11401 ccgccggggc cattgtgcgc cggatcggcc aatcgttaca aattgtctcc aaaccagacc
    11461 ggaggtgggc catcttgcag tcggttacga ggttcttcca tccccaaaga ggaggagtcg
    11521 ccgcccaggc cacgatcctg cggctcggca agacaaggtt ttggctccga gcggcaggcg
    11581 ccgccgccgt cgccgccggg ctggcgggct gctcgcccgc gttggactgg cgcgacgtgc
    11641 gcctgcccga caccgcgctg gtgacgcagc tgccctgcaa gccgggccgg ttcggacgca
    11701 aggtcgaggt cgcgggccgg ccgctgcagc tgttcatgct gtcgtgcgag gccggcggcg
    11761 tgacctatgg cgtcgccacg gccgagatcg gcgatccggc gcaggtgccc gcggtgctcg
    11821 acgggctggc gagcgcggcc gtgtccgccc tgcacgcggc cgaaccgcgc ttcgcaccgt
    11881 ttgcgatgga gggcgtgacg cccttccatg aaaatgtgag cgctcgcctc catggggtgc
    11941 gccccgacgg ccaggcggtc gaggaatcga tccgtgtctt cgcgcgcggc acgcgggtct
    12001 tccaggcgag cgcgatcggc gccgccctgc ccgatccagc cgtgcagccg ttccacgagg
    12061 gcttgcgctt cgacctggaa agaaaggacg ccggccccag ctgatgcggg tgcgcccgtc
    12121 cggattccct cgaagtgtgc aaatcggcca cgaaaccgcc cccccgatcg gggagacttc
    12181 cacatgcagg ctaggctcgg cggtggacgg ggccgataat cattcgtttg cgtttgttca
    12241 tccagtcgcc tggatctgag ccgtggccgc cgtcctgctg atcgcccatg cacccctcgc
    12301 gtccgccttg cgcggcgtcg ccgggcattg ctttgccgag aaggggcatc tcttcgaggc
    12361 ggtcgacgtc gatcccacga tgtcggccga cgacgccgag gcgctcgcgc gcgcggcggt
    12421 cgcgcgactc gcgtcccccg aggtgctgat cctcaccgac gtgttcggcg ccacgccctg
    12481 caacgccgcc cagcggcttg ccgacggcgc ggcggtgcgg gtggtcgcgg gcgtgaacgt
    12541 gccgatgctg tggcgctcgc tgtgctacct tgccgagccg ctcgacgcgc tcgtcgcgcg
    12601 cgccgtctcg ggcgccacgc agggcgtcct gcaggtggcc cagacccggc cacagaacca
    12661 gaacccatcc atttcatcga atgatccgga ccacgctcgt catcagcaat aagctgggcc
    12721 tgcacgcccg cgcctcggcc aagctcacaa agctggccac cagtttcaag agcgaggtgt
    12781 tcatgagccg caacggtcgt cgcgtgaacg ccaagagcat catgggcatc atgatgctgg
    12841 cagcgggcct gggctcggaa gtcgagctgg agaccgaggg ccccgacgag caggaggcca
    12901 gcgacgcgat ccgcgcgctg gtcgacgaca agttcggcga agggcagtga ggccgcgcgc
    12961 gggccggatc ccgcgcgacc gggacgagaa cggcgccgcg gcggcggatc aggccgcgag
    13021 gaagtggcgc cggtagcgct cgggcatgtc cgcgacgcgc gtgagcatcg gcaggtcggc
    13081 ggtgccgaac tccggatccc aggccggcgc gcccagcacc ttcgcgccca ggcgcaggta
    13141 gcccttgatc agcgcgggcg gctcgacgtc cagtccttgc tcgaggtcgc ccacgggcag
    13201 cggcactcgg gggcgcacct gccactccag acccgcgagg tgggtctcgc gcagcttggc
    13261 ccagaggctc gcggcgcagt ggccgccgtc gcgcatcgac aggctcgcgc agccgatcac
    13321 ggtctccatc ccgttgcgct ccatgaactg cgcgagcgcg ccccacagcg cgaggatcac
    13381 cccgcccgag cgatggtccg gatgcacgca gctgcggccc agttccacca tgcgcgggcg
    13441 cagcgggcgc aggcgcgtga ggtcgaactc ggtgtcgctg tagaagctgc cggcgcgccg
    13501 ggcggcggcg ggagtcagca ggcggtaggt gccgacgacc tggcccggct cgtcggccgc
    13561 cggggtggtg cgcacgatca ggtgctcgca gaactcgtcg aagacgtcga tgtcgtgccc
    13621 cgccggcgct ccgaccagcg gggtcggccg ggcgcccatc tcttcgacga agacctggtg
    13681 acgcaggcgc tgcgcggcgc gcacgtcgtc gaggtgggtg gcccagacgg cgtgcagctg
    13741 cggcgcgacg tgcgagcgct gcgtgcgtcc ggcggcgcgc tcggggcggc gcaggccgtc
    13801 cagcgtcgcg gcgccgatcg cgttgcgcaa ttcactcatt caattctccc gaatctacgg
    13861 cggtgatggt cacgggggcg gatgacgatg ccgtgaaccg gcggtgacgc gcccatgacg
    13921 gcgctgcgtg cggggcacgg cccggcacgg cgcggccggc ttgcgctaca ttggccggat
    13981 gggcttgccg ggccgcggtg cggcggcggg tggtcaggcg gaaaggggct tcgtgagcat
    14041 tcaggtgttc ggtcttcccg tgtcgcgcgg cgtcgcgatc gggcgcgcgg tgctggtggc
    14101 gtccagtcgc gtcgacgtcg cgcactactt catcgagccg gcgctgatcg aggcggaggt
    14161 gctccgcctg cgcgcggcgc gcgacctcgt ggtcgaagaa ctcaccgcac tgaaggagga
    14221 cgtgcccgag gacgcgcacg acgagctcgt cgcgctgctc gacgtccacc tgatgctgct
    14281 gcaggacgag acgctcgccg acgcgacgcg ccagtggatc gtcgagcgcc actacaacgc
    14341 cgagtgggcg ctctcgtcgc agctggaggt gatcgcgcgc cagttcgacg agatggagga
    14401 cgagtacctg cgcgaccgca agggcgacct cgagcaggtg gtcgagcgcg tcatcgctgc
    14461 gctggggcgt ctggcgggcg actcgtttgc cgaacccccg ccgcattcgc cgcgcgactt
    14521 cgctggcgag gatccgctcg tgctcgtggc caacgacatc gcgccggcgg acatgctgcg
    14581 tttccgccgg agcgtcttca cgggcttcgt caccgacgtc ggagggcgca attcgcacac
    14641 ggccatcgtg gcgcgcagca tggacatccc cgccgtcgtc ggggcccgcg aggcgagccg
    14701 catcgtcacg caggacgact ggatcatcat cgacggcgac gccggcgtcg tcatcgtcaa
    14761 tccctcaccg atcgtgctcg aggaataccg cttccgccaa cgccaggtcg agctcgagcg
    14821 tgcacgcctg gatcgcctgc gcaatacccc cgcggtgacg atggacgggc aggcgatcga
    14881 gctgctcgcc aacatcgagc tgccgagcga cgttcaggcg gtgctcgacg cgggcgcagt
    14941 cggcgtcggc ctgttccgca gcgagttcct cttcatgaac cgcaacggcc gcctgcccgg
    15001 cgaggaggag cagtaccagg cctaccgccg cgtggtcgaa gggctggacg ggctgccggt
    15061 gaccatccgc acgatcgaca tcggcgccga caagccgctc gaccgcgacc aggacggccg
    15121 cgaccgcgac cacgcgctga acccggctct gggcctgcgc gccatccgct ggagcctgtc
    15181 cgagccggag atgttcggcg cacagctgcg cgccatcctc cgggcgagcg cgctcggcaa
    15241 ggtgcggctg ctggtgccga tggtggcgca cctgcgcgag gctcgcctgg tgctcgaggc
    15301 gatcgagcgg gcgaaggcgc agcttcgcgc gcgcgcgcag gcgtacgtcg acgtgcaggt
    15361 cggcgccatg atcgaggtgc cggcggccgc catcctgctg ccgtcgctgc tgaagtactt
    15421 cgacttcatc tcgatcggca ccaatgacct gatccagtac acgctggcca tcgatcgcgc
    15481 cgacgaatcg gtctcgcacc tctacgaccc atggcatcct gccgtgctgc agctgctgtc
    15541 gcagacgatc gcgaaggcca acgagctggg caaggaggtc tgcgtctgcg gcgagatggc
    15601 tggcgacccg gcgttcaccg agctgttgct ggggatggga ctgcgcagct tctcgatgca
    15661 tccgacgcag gtgtcctcga tcaagaagcg catcctgcgc gtcgatacga aggcgtggag
    15721 cgagcgcatc gacctgatcg tcaacgcgga cgatccacag gccatctgcg cccggctgca
    15781 tacggctgcc ccggcaccgg acggcgcctg aggcgggcgg aagcccgctc aactttttcg
    15841 cggaaggggc ttgcgctccg acaaaggggt gtcctagaat agcaggctcc gctgatcgaa
    15901 agacggcgga gggagcgaga agctcggtga ttgcgaaagc gatcggcgtt gaaagacgaa
    15961 ataaccgagc agttgacaag gttttaaaaa caagtcaaaa tcttatttct ctgctgatca
    16021 ctggtcggca acgcagcgaa agccgcgaag ttctttaaaa atcaacagcc gataagcgtg
    16081 ggcgtttgaa ggcgagtgcc aagagtcctt cgggattcga agtcatatga ctttaaacgc
    16141 tcatggaaat tgaagatagc ttgcaaaagt ttcttcgatt cctttatg
//
LOCUS       DASVLN010000116         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_381740, whole genome shotgun sequence.
ACCESSION   DASVLN010000116 DASVLN010000000
VERSION     DASVLN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381740"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VF453_02575"
     CDS             <1..363
                     /locus_tag="VF453_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020693807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686559.1"
                     /translation="ASASPAAPAGPQPVKVKSRDGSFDGEMIGTPAPGSKFAKVKIGM
                     EFNEVSALIGGPSSLDSHETGKRWIPFYFGNDARRMEAIYNGEGCLTFTAGNVWGGGG
                     NELIRITADPTGKLCKDE"
     gene            complement(463..1242)
                     /locus_tag="VF453_02580"
     CDS             complement(463..1242)
                     /locus_tag="VF453_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7686560.1"
                     /translation="MSSTPSRTHLVLIPSYNTGPKVLETVRDARARWNPVWVVVDGST
                     DGTGRALQDMAAADPGLRVLVLPRNQGKGAAVLHGLREARAAGFTHALTMDSDGQHPA
                     SLIPRFMLDSIERPETMVLGRPVFDASAPLLRVRGRRVSNWWTNLETLGAGVADSLYG
                     FRVYPVDDLIAVMARQPWMRRFDFDTEAVVRLAWRGVKPVNVDAPVKYLTAEEGGVSH
                     FRYGRDNVLLTWMHLRLMLEFALRLPVLAWRKATGRAPFQD"
     gene            complement(1239..2024)
                     /locus_tag="VF453_02585"
     CDS             complement(1239..2024)
                     /locus_tag="VF453_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7686561.1"
                     /translation="MRTLLAVPLTLALYFMLAILGAISLGWCAIAMLLYPLLPRERGK
                     AIGRAGIARGYAFFWRLAGWSGMLRIESQALDALRDERGLIVVANHPSMLDALILIAR
                     LPRGACIMKAELQRNVFLGAGARLARYIRNDSPRKLVKLAVQDLKSGGQLVLFPEGTR
                     TTGPSVDRFCPGATTIAKLAGAPIQTVFIETDSPYLRKGWPIWRLPPLPMVYRVRLGR
                     RYQPARDADALLREIEQYFAANLQPATARVALPARREDAVPTP"
     gene            complement(2021..>2629)
                     /locus_tag="VF453_02590"
     CDS             complement(2021..>2629)
                     /locus_tag="VF453_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686562.1"
                     /translation="AAAFEGAPAACAIGAGAGPLCVRVLAGRVPPQPIENPRQVSAYN
                     YPVTYGPRSPTFSRAAAVQADASEVALFVSGTASIVGHQTVHAGDVLAQTRETLVNLQ
                     TVIAGANARGSAVFELPALDCVVYVRRPGDLALVRATFEEAVGAGSPAARGAVYVRAD
                     ICRSDLLVEIEAHGFAAGVLRAAPVEPGVDLRQARAAAGDAP"
BASE COUNT          382 a          951 c          978 g          318 t
ORIGIN      
        1 gccagcgcca gccccgccgc gcccgccggc ccgcagccgg tcaaggtcaa gtcgcgcgac
       61 ggcagcttcg acggcgagat gatcggcacg cccgccccgg gcagcaagtt cgccaaggtc
      121 aagatcggca tggagttcaa cgaggtctcg gcgctgatcg gcggcccgag cagcctcgac
      181 tcgcacgaga ccggcaagcg ctggatcccg ttctacttcg gcaacgacgc gcgccgcatg
      241 gaagcgatct acaacggcga aggctgcctg accttcacgg ccggcaacgt ctggggcggc
      301 ggcggcaacg agctgatccg catcaccgcc gacccgaccg gcaagctctg caaggacgag
      361 tgatcgctcc gcgccggcct tcccgggccg cgcaactgga cggcgccgcg gccttcgccc
      421 ggcgccgtct tgctttccgg aatgcctgcc tggcgacggc gctcagtctt ggaacggcgc
      481 ccgtcccgtc gccttgcgcc atgccagcac cggcagccgc agcgcgaact ccagcatcag
      541 gcgcaggtgc atccacgtga gcagcacgtt gtcgcgcccg tagcggaagt gcgagacgcc
      601 gccttcctcg gcggtgaggt acttgaccgg cgcatcgacg ttgaccggct tgacgccgcg
      661 ccacgcgagc cgcacgacgg cctcggtgtc gaagtcgaag cggcgcatcc agggctggcg
      721 cgccatcacc gcgatcaggt cgtcgaccgg gtagacgcgg aagccgtaca gcgagtcggc
      781 gacgccggcg cccagggtct ccaggttggt ccaccagttg gagacgcggc gcccgcgcac
      841 gcgcagcagc ggcgcgctgg cgtcgaagac ggggcggccg agcaccatgg tctcgggacg
      901 ctcgatcgag tccagcatga agcgcgggat cagcgacgcg ggatgctggc cgtcggagtc
      961 catcgtcagc gcgtgcgtga agcccgccgc gcgcgcctcg cgcaggccgt gcagcaccgc
     1021 cgcgcccttg ccctggttgc gcggcagcac cagcacgcgc aggccgggat cggcggcggc
     1081 catgtcctgc agcgcgcggc cggtgccgtc ggtggagccg tcgacgacca cccacaccgg
     1141 gttccagcgg gcgcgcgcgt cgcgcacggt ctcgagcacc ttggggccgg tgttgtagct
     1201 cgggatgagg acgaggtggg tgcgggacgg cgtcgagctc atggcgtcgg cacggcgtcc
     1261 tcgcgccgcg caggcagggc cacgcgggcg gtggccggct ggaggttggc ggcgaagtac
     1321 tgctcgatct cgcgcagcag cgcgtcggcg tcgcgcgccg gctggtagcg gcggccgaga
     1381 cggacgcggt agaccatggg cagcggcggc agccgccaga tcggccagcc cttgcgcagg
     1441 taaggcgagt cggtctcgat gaagacggtc tggatgggcg cgccggccag cttggcgatg
     1501 gtcgtcgcgc ccgggcagaa gcggtcgacg ctcgggccgg tcgtgcgcgt gccctcgggg
     1561 aacagcacca gctgtccgcc cgacttcagg tcctgcaccg cgagcttcac gagcttgcgc
     1621 ggcgagtcgt tgcggatgta gcgcgccagc cgcgcccccg cgccgaggaa gacgttgcgc
     1681 tgcagctcgg ccttcatgat gcaggcgccg cgcggcagcc gcgcgatcag gatcagcgcg
     1741 tccagcatgc tcgggtggtt ggcgacgacg atcaggccgc gctcgtcgcg cagcgcgtcg
     1801 agcgcctgcg attcgatgcg cagcatcccc gaccagccgg cgagccgcca gaagaacgca
     1861 tagccacgcg cgatgcccgc gcgtccgatc gccttgccgc gctcgcgcgg cagcagcggg
     1921 tacaagagca tcgcgatcgc gcaccagccc agcgagatcg cgccgaggat ggcgagcatg
     1981 aagtacagcg ccagcgtcag cggcaccgcc agcagcgtcc tcacggggcg tcccccgccg
     2041 cggcacgcgc ctgccgcagg tcgacgcccg gctcgaccgg cgccgcgcgc aggacgcccg
     2101 ccgcgaagcc gtgggcctcg atctccacca gcaggtcgga gcggcagatg tccgctcgca
     2161 cgtagaccgc gccgcgggcg gccggcgagc cggcgccgac cgcctcctcg aaggtcgcgc
     2221 gcacgagggc gagatccccg ggccggcgca cgtagaccac gcagtcgagg gccggcagct
     2281 cgaagacggc gctgccgcgc gcgttcgcgc cggcgatcac ggtctgcagg ttgacgagcg
     2341 tctcgcgcgt ctgggcgagg acgtcgccgg catgcaccgt ctggtggccg acgatgctgg
     2401 cggtgccgga gacgaacaac gcgacctcgc tcgcgtcggc ctgtaccgcg gccgcgcgcg
     2461 agaacgtcgg gctgcgcggc ccgtacgtca ccgggtagtt gtacgccgag acctggcgcg
     2521 ggttctcgat cggctgcggc gggacgcggc cggcgagcac gcgcacgcac agcggccccg
     2581 cgccggcgcc gatcgcgcag gccgcgggcg cgccctcgaa ggcggccgc
//
LOCUS       DASVLN010000117         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_435869, whole genome shotgun sequence.
ACCESSION   DASVLN010000117 DASVLN010000000
VERSION     DASVLN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435869"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..3472
                     /locus_tag="VF453_02595"
     CDS             218..3472
                     /locus_tag="VF453_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7686563.1"
                     /translation="MTPLPWKGGRPVASAIATALALTIAGCGGGDAAAPAQAQETTQA
                     AATAATQAASAPAPLPQLADWPKLGSAFPKEPDIEARVDALLGQMTLEEKVGQMTQAE
                     IQYVTPAEVLQYHLGSVLNGGGSWPGGDKGAPVQAWLDMADGLWTAAMSDRLHIPLLW
                     GTDAVHGHNNVKGATMFPQNIGLGAMRDPELMGRIGKVTALEVARTGIDWAFGPTVAV
                     VRDDRWGRTYEGYSEDPEVQQSYAGRVVTGMQGRLRKDAAVNQKVIATAKHFLGDGGT
                     TNGTDQGVTVATEAQLINIHGRGYYSAIQAGVQAIMVSYSSWQDAAQGPDAKALKMHG
                     NQYLLTDVLKNKMGFDGIVVTDWNGIGQVKTTNSSSPRDCTTNDCPQAINAGVDMVMA
                     PTRADWIPFIANTIASVKAGEIPMSRIDDAVRRILRVKLRMGLMDRPKPSLRNASHAI
                     GTPEDRAVAREAVRKSIVLLKNNGSALPIARGAKILVAGKSADSLANQAGGWSITWQG
                     GNANADFGGGTTLWGAVQKVAPQAVLDPSPDAKLADPSVDVAIVAIGETPYAEGAGDI
                     GPSKTLELAKLHPEDVALLNALRAKGVKRIVTVLYSGRPLYVDQELNRSDAFVAAFLP
                     GTEGDGLADVLFQKAGGGTNFDFNGKLSYSWPRSACQTSQDRFAPDYSPLYAYGYGLT
                     YASHVVQGQYDETSATHGCGQSDPGSATQPLAIFDRTNQGNWQMQVGASSNWDVPVAL
                     STASTTTTPGGEISAVPVDDQNGIQWAALLVTWNNAYGEFFSQDAANSKVDLSAYAGT
                     AGSLAFDIKVLQAPTDAVNLRLDCNYPCRGQADATAAVKALGVGTWGTLSVSLDCLAE
                     RGADLGIINRPFLMSSSGRFQAAIANIRWEPNKPGNVSCTSPKVPLDADADVYVDGVS
                     NTDLFDPPRVWTSGGSGTATLDTQFVAEDGRKVLDVRIANQLETGGNAGFAIDAKAGT
                     QLDVSAMAATGGVEYDVRVLDYGGMTQGFWTKLVCNSAPDSCRTGDLKDLIGRPPLGT
                     WLHVKLPYSDPSYAANGWDPGKLTSGVEVLPAWGDQAGSIHYQLRDIRVKKNLH"
     gene            3484..4149
                     /locus_tag="VF453_02600"
     CDS             3484..4149
                     /locus_tag="VF453_02600"
                     /inference="COORDINATES: protein motif:HMM:NF038127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DVUA0089 family protein"
                     /protein_id="HEX7686564.1"
                     /translation="MKAIRTLLASAALVAAGSLAHAAPVAVTGSNHAFGDAFDVPASA
                     FDTGADPDIDDAATVPHASVVDLAAGPLDYYRVTLAAAGTLTLDVDYGDGWDHAGLAL
                     DTKLAVWTSGGALLAVDDDDPRPWGFPGPSADAGSSSTLDPWLQLGGLAAGSYVIGVA
                     YSGADAADGGWLPGGEIPPGGMYELNVSVGPSSAPVPEPAPALAWVAGALTLALARRR
                     QGR"
     gene            complement(4212..4400)
                     /locus_tag="VF453_02605"
     CDS             complement(4212..4400)
                     /locus_tag="VF453_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686565.1"
                     /translation="MDEAPVGAARCERCELVLALVELACVLCALQECLEELALVLDVA
                     SAREAAAVVAAASRRSPA"
BASE COUNT          716 a         1656 c         1559 g          585 t
ORIGIN      
        1 ccagtacggc tcgatgctct ggcgtgagcg tcggcggtcg tccgccctgt ctcggcttgt
       61 cgttcatcag tcacctcgca acttcagcta ttgtgcaagc tgtagcgtgt gaagtgttga
      121 tttcagctac accctctgag gcccgccacc ccgtcggcgc gccacgggac gcggcagggc
      181 gcgccccgat gacgaccctc cccaggagac agcagcgatg accccccttc catggaaagg
      241 cggccggccg gtcgcctcgg cgatcgccac ggcgctcgcc ttgacgatcg ccggatgcgg
      301 cggcggcgat gccgcggccc cggcgcaggc gcaggagacg acgcaggccg ccgcgacggc
      361 cgcgacccag gctgcttccg cgccggctcc gctgccgcag ctcgccgact ggccgaagct
      421 cggcagcgcc ttccccaagg aacccgacat cgaggcccgt gtcgacgcgc tgctcgggca
      481 gatgacgctc gaagagaagg tcggccagat gacccaggcc gagatccagt acgtgacgcc
      541 ggccgaggtg ctgcagtacc acctgggctc cgtgctcaac ggcggcggct cctggcccgg
      601 cggcgacaag ggcgcgccgg tgcaggcctg gctcgacatg gccgacggcc tgtggaccgc
      661 ggcgatgagc gaccgcctgc acatcccgct gctgtggggc acggacgccg tgcacgggca
      721 caacaacgtc aagggcgcga cgatgttccc gcagaacatc gggctgggcg cgatgcgcga
      781 ccccgagctg atggggcgca tcggcaaggt gacggcgctg gaggtcgcgc gcaccggcat
      841 cgactgggcc ttcgggccga ccgtcgccgt ggtgcgcgac gaccgctggg gccgtaccta
      901 cgaggggtat tccgaagacc ccgaggtgca gcagtcctac gccggccgcg tggtcaccgg
      961 catgcagggc cgcctgcgca aggacgccgc ggtcaaccag aaggtcatcg cgaccgccaa
     1021 gcatttcctc ggcgacggcg gcacgacgaa cggcaccgac cagggcgtca cggtggcgac
     1081 cgaagcgcag ctgatcaaca tccacggccg cggctactac tcggcgatcc aggccggcgt
     1141 gcaggccatc atggtctcgt acagcagctg gcaggacgca gcccagggcc cggacgccaa
     1201 ggcgctgaag atgcacggca accagtacct gctgaccgac gtgctgaaga acaagatggg
     1261 cttcgacggc atcgtcgtca ccgactggaa cggcatcggc caggtcaaga cgaccaacag
     1321 ctcgtcgccg cgcgactgca ccaccaacga ctgcccgcag gccatcaacg ccggcgtcga
     1381 catggtgatg gcgccgacgc gcgcggactg gatccccttc atcgcgaaca cgatcgcgag
     1441 cgtgaaggcg ggcgagatcc cgatgtctcg catcgacgac gccgtgcgcc gcatcctgcg
     1501 cgtcaagctg cgcatgggcc tgatggaccg gcccaagccc tcgctgcgca acgcgtcgca
     1561 cgcgatcggc acgcccgagg accgcgcggt ggcgcgcgag gcggtgcgca agtccatcgt
     1621 gctgctgaag aacaacggct cggccctgcc gatcgcccgg ggcgcgaaga tcctcgtcgc
     1681 cggcaagagc gcggacagcc tcgccaacca ggcgggcggc tggtcgatca cctggcaggg
     1741 cggcaacgcg aacgcggact tcggcggcgg caccacgctg tggggcgcgg tgcagaaggt
     1801 cgcgccacag gcggtgctcg acccgagccc cgacgcgaag ctcgccgacc cgagcgtcga
     1861 cgtcgcgatc gtggccatcg gcgagacgcc ctacgcggag ggcgccggcg acatcgggcc
     1921 gtcgaagacc ctggagctcg ccaagctgca tcccgaggac gtcgcgctgc tgaacgcgct
     1981 gcgggccaaa ggcgtgaaga ggatcgtcac ggtgctgtac tcgggccgcc cgctgtatgt
     2041 cgaccaggag ctgaaccgct ccgacgcctt cgtcgccgcg ttcctgcccg gcaccgaagg
     2101 cgacggcctc gcggacgtcc tgttccagaa ggccggcggc ggcaccaact tcgacttcaa
     2161 cggcaagctg tcttactcgt ggccaaggtc cgcctgccag accagccagg accgattcgc
     2221 cccggactac tcgccgctgt acgcgtacgg atacgggttg acgtatgcat cgcacgtcgt
     2281 ccaaggccag tacgacgaga ccagcgcgac ccacggctgc ggccagtccg accccggcag
     2341 cgcgacccag ccgctggcga tcttcgaccg cacgaaccag ggcaactggc agatgcaggt
     2401 cggcgcatcg agcaactggg acgtgcccgt cgcgctgtcg accgcgtcca ccacgacgac
     2461 gccgggcggc gagatctcgg cggtgccggt cgacgaccag aacggcatcc agtgggccgc
     2521 gctgctcgtg acctggaaca acgcctacgg cgagttcttc tcgcaggacg ccgcgaacag
     2581 caaggtcgac ctctcggcct acgccggcac cgcaggctcg ctcgcgttcg acatcaaggt
     2641 gctgcaggcg cccaccgacg ccgtgaacct gcgcctggac tgcaactacc cctgccgcgg
     2701 ccaggccgac gcgacggccg ccgtgaaggc gctgggcgtg ggcacctggg gcacgctgag
     2761 cgtctcgctc gactgcctgg ccgagcgcgg ggccgacctg ggcatcatca accggccctt
     2821 cctcatgagc tccagcggcc gcttccaggc cgcgatcgcc aacatccgct gggagccgaa
     2881 caagccgggc aacgtgagct gcacgtcccc gaaggtgccc ttggacgcgg atgccgacgt
     2941 gtacgtggac ggggtatcca acaccgacct gttcgacccg ccgcgcgtgt ggacatcggg
     3001 cggcagcggc accgcgacgc tggacaccca gttcgtcgcc gaggacggtc gcaaggtgct
     3061 ggacgtgcgc atcgccaacc agctcgagac cgggggcaat gcgggcttcg cgatcgacgc
     3121 caaggccggc acgcagctgg acgtctcggc gatggcggcc accggcgggg tcgagtacga
     3181 cgtgcgcgtc ctggactacg gcggcatgac gcagggcttc tggacgaagc tcgtgtgcaa
     3241 cagcgcgccc gactcgtgcc gcaccggcga cctgaaggat ctcatcggcc gcccgccgct
     3301 cggcacctgg ctgcacgtga agctgcccta cagcgacccg agctacgcgg cgaacggctg
     3361 ggatccgggc aagctcactt ccggtgtcga ggtgctgccc gcctggggcg accaggccgg
     3421 ctcgatccac taccagctgc gcgacatccg cgtgaagaag aacctgcact gagaggctcc
     3481 gccatgaaag cgatccgcac cctcctcgcc tcggccgcgc tcgtcgccgc cggctcgctc
     3541 gcccacgccg cgcccgtcgc ggtgaccggc tccaaccacg ccttcggcga cgcgttcgac
     3601 gtcccggcct ccgcgttcga caccggcgcc gatcccgaca tcgacgacgc ggccaccgtg
     3661 ccgcacgcct ccgtcgtcga cctcgccgcg gggccgctgg actactaccg cgtcacgctg
     3721 gccgcggccg gaacgctgac gctggacgtc gactacggcg acggctggga tcacgccggc
     3781 ctcgccctcg acacgaagct cgccgtctgg acgtccggcg gcgcgctgct ggcggtcgac
     3841 gacgacgacc cgcgaccctg gggtttcccg ggaccgtcgg ccgacgccgg cagctcctcg
     3901 acgctcgatc cctggctgca gttgggcgga ctggccgcgg gcagctacgt gatcggcgtc
     3961 gcgtactccg gcgcggacgc cgcggacggt ggctggctgc cgggtggcga gataccccct
     4021 gggggcatgt acgagctcaa cgtctcggtc ggcccgtcaa gcgccccggt tcccgagccc
     4081 gcgcccgcgc tcgcctgggt cgcgggcgcg ctgaccctgg cgctggcgcg tcggcggcag
     4141 ggtcgctgag ccgaggcggc agttccggat cgaaggcctt tccgatcgcg cccggcgtgc
     4201 ccggggcgcg gtcaggcggg cgagcgtcgc gaagccgctg cgacgaccgc ggctgcctcg
     4261 cgcgcggacg cgacgtccag caccagcgcg agctcctcca ggcattcctg caaggcgcac
     4321 aacacgcatg cgagctcgac cagcgcgagg acgagctcgc agcgttcgca gcgcgccgcg
     4381 ccgacgggcg cctcatccat gcgccgtccc ggcatcgcgt accgcgtcct gcgcgagcac
     4441 ggtcgcgtcg cgggccgcct cgtcgacctg cgcggccgcg cgagattcgc gcatcagcca
     4501 ttcgagcgca cgcgcg
//
LOCUS       DASVLN010000118         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_113327, whole genome shotgun sequence.
ACCESSION   DASVLN010000118 DASVLN010000000
VERSION     DASVLN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113327"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VF453_02610"
     CDS             <1..433
                     /locus_tag="VF453_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HEX7686566.1"
                     /translation="AASAAPAAGAQLRLVGPASVRAGESFDVTLDLDAAAALRGMPVQ
                     LSYDRAHLALETIEEGGYFAQDGARTSFTQNIQAAEGIARAGVLRNAATSVAGHGTVY
                     KLRFKALAAGGGTIAVTGVNPIGLGGEVAVATPAPLAVAVK"
     gene            436..912
                     /locus_tag="VF453_02615"
     CDS             436..912
                     /locus_tag="VF453_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7686567.1"
                     /translation="MRARRGFSLVELMAVLAILAVLAWAILPLAEVSREREREHELQR
                     ALWQIRDAIDAYKKVSDASMPASAPRAGGVSGYPPTLAALVQGLPDPQASGRTIYFLR
                     RVPRDPFAPDALPADQTWGLRSFASPADAPAPGADVYDVHSLAEGTGLNGVPLRQW"
     gene            915..1301
                     /locus_tag="VF453_02620"
     CDS             915..1301
                     /locus_tag="VF453_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7686568.1"
                     /translation="MATRRHRARGFTLIELLVVMAVIGLLLSIAAPRFVQRVDQSRDV
                     VLRHNLAGLRAAIDEFHTDKGRYPKTLQELVDQRYLRSVPVDPLTERADTWRMVPQPD
                     ATEAAWFDVHSGAPGVAGDGTTYASW"
     gene            1339..1764
                     /locus_tag="VF453_02625"
     CDS             1339..1764
                     /locus_tag="VF453_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX7686569.1"
                     /translation="MLAILAILAIGLAAVGTRWSDRAQRERERQLLRVGRLYAEAIDA
                     YHHGSPGSDKTWPREIDDLLLDPRMLGTVRYLRAAYTDPMMPGAPFGLVRAADGTIRG
                     VYSTSTAQPFTQVTVDLGVTTLAPAHRYADWQFVPKEDS"
     gene            1761..2189
                     /gene="gspG"
                     /locus_tag="VF453_02630"
     CDS             1761..2189
                     /gene="gspG"
                     /locus_tag="VF453_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX7686570.1"
                     /translation="MTRHSRPHAASRGFTLMELLVVILIIGLLTGIVGPRLLGQISRS
                     EVTTARAQMDAFDKALQAYRLDCGHFPSSSQGLQALVTQPADETRWHGPYLQGTVPVD
                     PWGTPYQYRSPGTSGKDFDLVSLGHDRAPGGTGDDADIVR"
     gene            2208..>3159
                     /locus_tag="VF453_02635"
     CDS             2208..>3159
                     /locus_tag="VF453_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7686571.1"
                     /translation="MSAPAPATAFDVRVLRPGTSAVETLRLEAGSEAELRARLSAAGD
                     VVVGLKRASAAARGDAPTRGLGERRFDVGWWCRELRTLLLAGMTAVEAIETLAAGRHD
                     AERARIHAMLLEALRQGKSLSKAMAATGAFPAVLVAGVTASERTSTLPAALEDFLRYD
                     SLLRKLRQQLVSAAIYPAVVVTLGGAICLFLLLFVVPRFSRMYADLHGPVSGATEIVL
                     WVSRAVQDRWPLLAAGAAGLAVAGLAAWRAGWIARAGMVIVDAVPLLARQWREFRLAK
                     LYQALALMVKGGYAFDEALQVCAGLDGGARMAARVAEVRAA"
BASE COUNT          387 a         1165 c         1170 g          437 t
ORIGIN      
        1 cgccgcgtcc gccgcgccgg cggccggcgc gcagctcagg cttgtcggtc ccgcgagcgt
       61 ccgcgctggc gagagcttcg acgtcacgct cgacctggac gccgccgccg cgctgcgcgg
      121 catgccggtg cagctctcgt acgaccgcgc gcacctggcg ctcgagacga tcgaggaagg
      181 cggctacttc gcgcaggacg gcgcgcgcac cagcttcacg cagaacatcc aggccgccga
      241 gggcatcgcg cgcgccggcg tactgcgcaa cgcggccacc agcgtcgccg gccacggcac
      301 ggtctacaag ctgcgcttca aggcgttggc ggccggcggc ggcacgatcg ccgtgaccgg
      361 cgtcaatccc atcgggctgg gcggcgaggt cgcggtcgcg acgcctgcgc cgctggcggt
      421 cgcggtgaag tgagcatgcg agcgcggcgc ggcttctcgc tggtcgagct gatggcggtg
      481 ctggcgatcc tcgcggtgct cgcctgggcg atcctgccgc tggcggaggt gtcgcgcgag
      541 cgcgagcgcg agcacgagct gcagcgcgcg ctgtggcaga tccgcgatgc gatcgacgcc
      601 tacaagaagg tctcggacgc gtcgatgccg gcgtcggcgc cgcgcgcggg cggcgtgtcg
      661 ggctatccgc cgacgctggc ggcgctggtg cagggcctgc ccgatccgca ggcgagcggc
      721 cgcacgatct acttcctgcg ccgcgtcccg cgcgatccgt tcgcgcccga cgcgctgccc
      781 gcggatcaga cctggggcct gcgctcgttc gcgagtcccg ccgacgcgcc tgcgcccggc
      841 gcggacgtgt atgacgtcca ttcgctcgcc gaaggcacgg gcctgaacgg cgtgccgctg
      901 cggcagtggt gaccatggcc acccgccgcc accgcgcccg cggcttcacg ctgatcgagc
      961 tcctcgtggt gatggccgtc atcggcctgc tgctgtcgat cgccgcgccg cgcttcgtgc
     1021 agcgggtcga ccagtcgcgc gacgtcgtgc tgcgccacaa cctggccggg ctgcgcgcgg
     1081 cgatcgacga gttccacacg gacaagggcc gctacccgaa gacgctgcag gagctggtcg
     1141 accagcgcta cctgcgctcg gtgccggtcg acccgttgac ggagcgcgcc gatacctggc
     1201 gcatggtgcc gcagccggac gcgaccgagg cggcctggtt cgacgtgcac agcggcgcgc
     1261 ccggcgtcgc gggcgacggg acgacgtatg caagctggtg agcgcggccg cccgccggcg
     1321 ggattcgtct attttgcgtt gctcgcgatc ctggccatcc tggcgatcgg gctggccgcg
     1381 gttggcacgc gctggagcga ccgcgcgcag cgcgagcgcg agcggcagct gctgcgcgtc
     1441 ggccgcctct atgccgaggc catcgacgcg taccatcacg gctcgccggg cagcgacaag
     1501 acctggccgc gcgagatcga cgacctgctg ctcgacccgc gcatgctggg caccgtgcgc
     1561 tacctgcgcg ccgcctacac cgacccgatg atgccgggcg cgccattcgg cctggtgcgt
     1621 gcggccgacg gcacgatccg cggcgtctac agcaccagca cggcgcagcc gttcacgcag
     1681 gtgacggtgg acctcggcgt cacgacgctc gcgcccgcgc accgctacgc cgactggcaa
     1741 ttcgtcccga aggaagattc atgacccgac attcccggcc gcacgccgca tcccgaggct
     1801 tcacgctgat ggagctgctg gtcgtgatcc tgatcatcgg cctgctcacg ggcatcgtgg
     1861 ggccgcgcct gctgggccag atctcacgct cggaggtgac gaccgcccgc gcgcagatgg
     1921 acgccttcga caaggcgttg caggcctacc ggctggattg cgggcacttc ccgtcgagct
     1981 cccagggcct gcaggcgctc gtcacccagc ctgccgacga gacgcgctgg cacggcccct
     2041 acctgcaggg caccgtgccg gtagatccgt ggggcacgcc gtaccagtac cgctcgcccg
     2101 gcaccagcgg caaggacttc gacctcgtga gcctcggcca cgaccgcgcg cccggcggca
     2161 ccggcgacga cgcggacatc gtccgctgac cgggccccgg agcctcgatg tccgcccccg
     2221 cccccgccac cgcgttcgat gttcgcgtgc tgcgccccgg cacgtcggcg gtcgagacgc
     2281 tccgcctgga ggcaggctcc gaggccgagc tgcgcgcgcg cctgtccgcg gcgggcgacg
     2341 ttgtcgtcgg actgaagcgc gcgtcggcgg cggcacgggg cgacgcgccg acccgcggcc
     2401 tcggggaacg gcgcttcgac gtcggctggt ggtgccgcga gctgcgcacg ctgctgctgg
     2461 cgggcatgac ggcggtggag gcgatcgaga cactggccgc cggccgccac gacgccgagc
     2521 gcgcccgcat ccacgcgatg ctgctggagg cgctgcgcca gggcaagtcg ctctcgaagg
     2581 cgatggccgc caccggcgcc ttccccgcgg tgctggtcgc gggcgtgacg gccagcgagc
     2641 gcaccagcac gctgcccgcg gcgctggagg acttcctgcg ctacgacagc ctgctgcgca
     2701 agctgcgcca gcagctggtg agcgcggcga tctacccggc cgtcgtcgtc acgctgggcg
     2761 gcgcgatctg cctgttcctg ctgctgttcg tcgtgccgcg cttctcgcgc atgtacgccg
     2821 acctgcacgg ccccgtgagc ggcgcgacgg agatcgtgct gtgggtgagc cgcgcggtgc
     2881 aggatcgctg gccgctgctc gccgcgggcg ccgccgggct cgcggtggcg gggctggcgg
     2941 cttggcgcgc cggatggatc gcgcgcgccg ggatggtgat cgtcgacgcg gtgccgctgc
     3001 tcgcgcggca gtggcgcgaa ttccgcctcg ccaagctgta ccaggcgctc gcgctgatgg
     3061 tcaagggcgg ctacgccttc gacgaggcgc tgcaggtctg cgccggcctg gacggcggcg
     3121 cgcgcatggc cgcacgcgtc gccgaggtgc gcgccgcga
//
LOCUS       DASVLN010000119         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_6133, whole genome shotgun sequence.
ACCESSION   DASVLN010000119 DASVLN010000000
VERSION     DASVLN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6133"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VF453_02640"
     CDS             complement(<1..1493)
                     /locus_tag="VF453_02640"
                     /inference="COORDINATES: protein motif:HMM:NF022073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HEX7686572.1"
                     /translation="MKPSCRTVLASAALLAASLAPGAANAASVCERVLRQLGPELADA
                     TCVDSPDLTTNNPATTPANDSIPGLPPFAFTPQTDRATIAPSAAKRTPVTKAVPGIQL
                     QARIASDPTGQARFLLRLPADWNGRLVVAGASGTRSEFNGDFAWSDYVLQKGYAYASQ
                     NKGVLNLRLSSATDPLGCRLNPTSTVYVDFFDNGPGQPFARWQRFMVEAAKLARAGVA
                     AGYGRPPRFTYAVGTSNGGYQVRRAMETAPELFDGGVDWEGTFVDPHAPNLLTDLPAA
                     VLNFPDYVAGGLSPTSTAAKNLLAAGYPPDIVASPTSSLWTSYWAQFWEVTQCQWQKR
                     LDPAYDTYGAGTGNYNYVARLSASDVGAEMASFATTGRIQRPLVTVAGTMDGLLPIDH
                     HARAYARKVAEERAAHGHDGHAGWWNHEGAADADYRLYEIQNGNHIETFQDTFPQLAL
                     IEPQAQAAFDLLVAHVEARAPLPPDQCVPKGGTITRTPAEPGHCANL"
BASE COUNT          200 a          550 c          646 g          242 t
ORIGIN      
        1 tagaggttcg cgcagtgccc cggctcggcc ggcgtgcggg tgatggtgcc gcccttgggc
       61 acgcactggt cgggcggcag cggcgcgcgc gcctcgacgt gggccaccag caggtcgaac
      121 gccgcctgcg cctgcggctc gatcagcgcg agctgcggga aggtgtcctg gaacgtctcg
      181 atgtggttgc cgttctggat ctcgtagagg cggtagtcgg cgtccgcggc gccctcgtgg
      241 ttccaccagc cggcgtggcc gtcgtggccg tgcgccgcgc gctcctcggc caccttgcgg
      301 gcgtaggcgc gggcgtgatg gtcgatcggc agcaggccgt ccatcgtgcc ggcgaccgtc
      361 acgagcggcc gctggatccg cccggtggtc gcgaacgagg ccatctccgc gccgacgtcc
      421 gacgccgaca gccgtgcgac atagttgtag ttgccggtgc cggcgccgta ggtgtcgtag
      481 gccgggtcga gccgcttctg ccattggcac tgggtcactt cccagaactg cgcccagtag
      541 ctcgtccaca gcgaggacgt cgggctcgcg acgatgtcgg gcggatagcc ggcggccagc
      601 aggttcttcg ccgccgtgct ggtcggcgag aggccgccgg cgacgtagtc ggggaagttc
      661 agcaccgcag ccggcaggtc ggtcagcagg ttgggcgcgt gcgggtcgac gaaggtgcct
      721 tcccagtcga cgccgccgtc gaacagctcg ggcgcggtct ccatcgcgcg gcgcacctgg
      781 tagccgccgt tggacgtgcc gaccgcgtac gtgaaccgcg gcgggcggcc gtaacccgcg
      841 gcgacccccg cgcgcgccag cttcgcggcc tcgaccatga agcgctgcca gcgcgcgaag
      901 ggctggccag ggccgttgtc gaagaagtcg acgtagacgg tcgaggtcgg gttgaggcgg
      961 cagcccagcg gatccgtcgc gctggacagc cgcaggttga gcacgccctt gttctgcgag
     1021 gcgtaggcat agcccttctg caggacgtag tcgctccagg cgaagtcgcc gttgaattcg
     1081 ctgcgcgtgc ccgacgcgcc ggcgacgacg agccgcccgt tccagtcggc cggcaggcgc
     1141 aggaggaagc gcgcctggcc ggtcgggtcg gaggcgatgc gcgcctgcag ctggatgccg
     1201 ggcacggcct tggtcaccgg tgtgcgcttg gcggcgctcg gcgcgatcgt cgcgcggtcg
     1261 gtctggggcg tgaacgcgaa cggcggcagg ccggggatcg agtcgttggc gggcgtcgtc
     1321 gccgggttgt tggtcgtcag gtcggggctg tcgacgcagg tcgcgtcggc cagctccggg
     1381 ccgagctggc gcagcacgcg ctcgcacacg gaggcggcgt tcgcggcgcc gggggcgagg
     1441 ctcgcggcga gcagcgcggc gctcgcgagg acggtgcggc aggacggctt catcgagatc
     1501 tcccgtgcgg cgtcgaaatg cggcggacag tagcgcgccg agccggccag cgcaagggcc
     1561 gaccgcgatg cgtgggcttt ggcgtcgtcg ccctgcccgt cctcagcgca agcgtcgccg
     1621 gaccccggcc ccctcgcg
//
LOCUS       DASVLN010000120         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_435983, whole genome shotgun sequence.
ACCESSION   DASVLN010000120 DASVLN010000000
VERSION     DASVLN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435983"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1013
                     /gene="aat"
                     /locus_tag="VF453_02645"
     CDS             207..1013
                     /gene="aat"
                     /locus_tag="VF453_02645"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX7686573.1"
                     /translation="MAIRWLQPDTPLPPTSEALAADSEAPGLLAAGGGLAIARLRDAY
                     AHGVFPWYSPGQPVLWWSPDPRMVLRTDAFRMSHSLRKTLRRFLRTPGCELRFDHDVA
                     RVMRCCAETPRDGQDGTWIVPEMQLAYADWARAGDVHSIETWIDGELVGGLYGVNLGR
                     MMFGESMFSHRTDASKVALAALVAFCRSHGMPVIDCQQATGHLASLGAAPIPRDEFER
                     EVHALVARAPAPAWTYDVRIWEALDLHAAPAHAPSATPARAAAPTAAHSS"
     gene            1021..1794
                     /locus_tag="VF453_02650"
     CDS             1021..1794
                     /locus_tag="VF453_02650"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX7686574.1"
                     /translation="MTHPKEAPFASLQFYATAPYPCSYLPGRMARSQVATPGHLIHAE
                     AYSALVATGFRRSGVFTYRPYCDRCRACVPMRVPVAGFRPSRSQRRAWTAHADLKVRV
                     MRLAFVPEHYDLYLRYQAGRHAGGGMDHDSVDQYTQFLLQSRVNTRLIEFRAPDVDGE
                     PGALRMVAIADILSDGLSAVYTFYDPDVRGSFGTFAVMWQIEQARSLKLPYLYLGYWI
                     AESPKMAYKVQFRPYETLVDGRWQPGEAAPDAHPAAGVA"
BASE COUNT          227 a          717 c          612 g          258 t
ORIGIN      
        1 ggcgcgtgcc gcggccgccg cgccgtcggc gcccgcctcg acgccctccc tcctgccgcc
       61 gccgagcaag tcgggcatcc agttctgacg accgccgcgc cggcgcccgg cctgacgtcg
      121 ggcgcggcgc cggcgcgacg aaatgccgcg ctcgcttccg gacgaagccg ccatggccga
      181 ccggcgcgaa gtatgcttga cgcccgatgg cgatccgctg gctgcaaccc gacactccgc
      241 tgcctcccac ctcggaggcg ctggcggcag actccgaggc gcccggcctg ctcgcggccg
      301 gtggcggctt ggcgatcgcc cggctgcgcg acgcgtacgc acacggcgtg tttccctggt
      361 acagccccgg ccagccggtg ctctggtggt cgcccgaccc gcgcatggtg ctgcgcacgg
      421 acgcgttccg catgtcgcac tcgctgcgca agacgctgcg ccgcttcctg cgcacgcccg
      481 gctgcgagct gcgcttcgac cacgacgtgg cgcgcgtgat gcgctgctgc gccgagacgc
      541 cccgcgacgg ccaggacggc acctggatcg ttcccgagat gcagcttgcc tacgccgact
      601 gggcgcgcgc cggcgacgtc cacagcatcg agacctggat cgacggcgag ctcgtcggcg
      661 gcctgtacgg cgtgaacctc ggacgcatga tgttcggcga gtcgatgttc tcgcaccgca
      721 ccgatgcctc caaggtcgcg ctggcggcgc tggtcgcgtt ctgccgcagc catggcatgc
      781 cggtgatcga ctgccagcag gccacgggcc acctggcaag cctgggcgcg gcgccgatcc
      841 cgcgcgacga gttcgagcgc gaggtgcacg ccctcgtcgc ccgtgcgccc gcgccggcct
      901 ggacctatga tgtccgcatc tgggaggcgc tggacctgca tgccgcgccc gcccacgcgc
      961 cttccgccac gccggcccgc gccgccgcgc cgactgccgc ccattcctcg tgatcccgtc
     1021 gtgacccatc cgaaggaagc cccgttcgcg tcgctgcagt tctatgcgac cgcgccctac
     1081 ccctgcagct acctgcccgg gcggatggcg cgctcgcagg tcgccacgcc ggggcacctg
     1141 atccacgccg aggcctactc cgcgctcgtc gccaccggct tccggcgcag cggcgtcttc
     1201 acctaccggc cctactgcga ccgctgccgc gcctgcgtgc cgatgcgcgt gcccgtggcc
     1261 ggcttccggc cctcgcgaag ccagcgacgc gcgtggacgg cgcacgccga cctcaaggtg
     1321 cgcgtgatgc ggctcgcctt cgtgccggag cactacgacc tctacctgcg ctaccaggcg
     1381 ggccgtcacg ccggcggcgg catggaccac gacagcgtcg accagtacac gcagttcctg
     1441 ctgcagagcc gcgtcaacac gcgcctgatc gagttccgcg cccccgatgt ggacggcgag
     1501 cccggcgcgc tgcgcatggt ggcgatcgcc gacatcctca gcgacgggct gtccgccgtc
     1561 tacacgttct acgaccccga cgtgcgcggc agcttcggca ccttcgccgt catgtggcag
     1621 atcgagcagg cgcgctcgct gaagctgccc tacctgtacc tgggctactg gatcgccgag
     1681 agcccgaaga tggcctacaa ggtgcagttc cggccctacg agacgctcgt cgacggccgc
     1741 tggcagccgg gcgaggcggc gccggacgcg catcccgccg ccggcgtcgc ctgaagcgct
     1801 ggcgcgccgt ccgg
//
LOCUS       DASVLN010000121         4014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_436012, whole genome shotgun sequence.
ACCESSION   DASVLN010000121 DASVLN010000000
VERSION     DASVLN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436012"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="VF453_02655"
     CDS             complement(<1..1543)
                     /locus_tag="VF453_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7686575.1"
                     /translation="MSSAFSRNPLKILLLEDSRFDAEFVAEFVRANYPLAQLTVVADE
                     RAFRAALRDGLAGEPYDLVLSDHDVPGYGGAEALAHARQVAPRLPFIFVSGVIGEENA
                     VDLLKRGATDYVSKNRLSRLPLVVDRALQEVVERDARDRAERQLRRADATYGRVVDAL
                     QDYAVILLDRHGEIRDWNQGASTIFRQPREQVVGTHFERLFTPEDRASGIPGRELREA
                     RAVGSASDNRWMLRADGTRLRADGVLTALRDTEDPAGEVTGFCKILRDGTKAFEIGEA
                     LRAAKEEAERANRAKDRFLAVLSHELRTPLAPIASAARLLEREARVPPEYAGLLPMIR
                     RNVAYEARLIDDLLDVTAISSGNVPLQLAPVDMHALVEGVLEMMSDATAEKSIALAAH
                     WHADNERVLGDEAHLQRVLWNVVRNAIKFTPEGGHVDLHVDNVGSCWRLRCVDDGIGI
                     TAEAQEKIFSAFEQADGWVMKQFGGLGLGLAIARGLIEQHGGRLEVSSAGPGRGSTFT
                     LTLETL"
     gene            complement(1557..2015)
                     /locus_tag="VF453_02660"
     CDS             complement(1557..2015)
                     /locus_tag="VF453_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7686576.1"
                     /translation="MLKPILLVEDDARDLELTLVALERSQLANDVVVVRDGAAALDYL
                     NRAGEHAGRAEGNPAVMLLDLKLPKLNGLEVLKSVRETPALRSIPVVMLTSSHEESDV
                     VASYDLGVNAYVVKPVEFKEFVAAIADLGVFWAVLNEPPPGSAKASRRYE"
     gene            complement(2020..>4014)
                     /locus_tag="VF453_02665"
     CDS             complement(2020..>4014)
                     /locus_tag="VF453_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7686577.1"
                     /translation="WPADESPAPAGTLALEGGRFDASVHRRGGRAIVEFEPAVDGSGT
                     RSPIYGLARHVVPALQAARTVEELCELATRELKRLTGFGRCLAYRFDAEGHGEVIAES
                     LDAGYASYAGHHFPGSDIPPQARALYCLNYVRVIPDADYEPVPLLSSDPGVQANAIDL
                     SLAGLRSVSPVHLEYMRNMGTRASMSISIVAKGRLWGLVSGHHREARHLDFETRAACE
                     HLGRLLSLRVEAAEEHAEVAQRLELRRITLQLIAQRPDLDSTLMGLLDVPDAMLHLAR
                     AGGAAVVLNESCRLIGETPAAEHVIALGRRIVERGEDSFATDRLVEVWPDAAGWGDTC
                     AGVLALSISQVHRHLILWFRPAVARTIRWAGEPNKELRADGRIHPRRSFASWKEELRD
                     RALPWTPAELAAALELRQSLIAIVLRRAEELADLAGELGRANKELEAFSYTVSHDLRA
                     PMRHISGYVDLVLDVDSAVLSERAKRYLAHVKDASAYSGQLVDGLLDFSRMGRAAIKR
                     GRVDTRVLVAELVREFTRLQPERAIRWEVDPDLPVLHADPLLMQVALRNLVSNAVKYT
                     RDAASPHIRIRPVRRGSPGRPEEGLEVEDNGCGFDMKYVGKLFGVFQRLHQSDEFEGT
                     GIGLASIRRIVERHGGQVWATGELGRGATFGFALPRAPEN"
BASE COUNT          560 a         1417 c         1468 g          569 t
ORIGIN      
        1 cgagcgtctc gagcgtcagc gtgaaggtcg agccgcgtcc cgggccggcg ctcgagacct
       61 cgagccgccc gccgtgctgc tcgatcagcc cgcgcgcgat cgccaggccc agcccgaggc
      121 cgccgaactg cttcatcacc cagccatcgg cctgctcgaa ggcggagaag atcttttcct
      181 gcgcctcggc ggtgatgccg atgccgtcgt ccacgcaccg cagccgccag cagctgccga
      241 cgttgtcgac atgcaggtcg acgtggccgc cctcgggcgt gaacttgatc gcgttgcgca
      301 ccacgttcca gagcacccgc tgcaggtgcg cctcgtcgcc gagcacgcgc tcgttgtcgg
      361 catgccagtg cgccgcgagc gcgatggact tctccgcggt cgcgtcggac atcatctcga
      421 gcacgccctc gaccagcgca tgcatgtcca ccggcgccag ctgcagcggc acgttgcccg
      481 aggagatcgc ggtgacgtcg agcaggtcgt cgatcagccg cgcctcgtag gcgacgttgc
      541 ggcggatcat cggcagcagg cccgcgtact ccggcggcac gcgcgcctcg cgctccagca
      601 gccgcgcggc cgaggcgatc ggcgcgagcg gcgtgcgcag ctcgtgcgag agcaccgcga
      661 ggaagcggtc cttcgcccgg ttggcgcgct cggcctcctc cttggcggcg cgcagggcct
      721 cgccgatctc gaaggccttg gtaccgtcgc gcaggatctt gcagaagccg gtgacctcgc
      781 cggccggatc ctcggtgtcg cgcagcgccg tcagcacgcc gtcggcgcgc aggcgggtgc
      841 cgtcggcccg cagcatccag cggttgtcgc tcgcgctgcc caccgcgcgc gcctcgcgca
      901 gctcgcggcc gggaatcccg ctggcgcgat cctccggcgt gaacaggcgc tcgaaatggg
      961 tgccgacgac ctgctcgcgc ggctggcgga agatcgtgct cgcgccctgg ttccagtcgc
     1021 ggatctcgcc gtggcgatcg agcaggatca ccgcatagtc ctgcagcgcg tcgaccacgc
     1081 gtccgtaggt cgcatcggcg cggcgcagct ggcgttcggc gcggtcgcgg gcatcgcgtt
     1141 cgacgacctc ctgcagcgcg cggtcgacga cgagcggcag ccgcgacaga cggttcttgc
     1201 tgacgtagtc ggtggcgccg cgcttgagca ggtcgacggc attctcctcg ccgatcacgc
     1261 ccgagacgaa gatgaagggc agccgcggcg cgacctggcg cgcgtgcgcc agcgcctcgg
     1321 cgccgccgta gcccggcacg tcgtggtcgg acagcacgag gtcgtacggc tcgccggcga
     1381 ggccgtcgcg cagcgcggcg cggaaggcgc gctcgtcggc gacgacggtc agctgggcga
     1441 gggggtagtt ggcgcggacg aactcggcca cgaactcggc gtcgaagcgc gagtcctcca
     1501 gcagcaggat cttcagcgga ttgcgggaga aggcactcga cacggcgcgc tccgcttcat
     1561 tcgtagcggc gcgatgcctt ggccgacccc ggaggcggct cgttgaggac ggcccagaac
     1621 acgccgagat cggcgatcgc ggccacgaac tccttgaact cgaccggctt gaccacgtag
     1681 gcgttgacgc ccaggtcgta gctcgccacg acgtccgact cctcgtgcga ggacgtcagc
     1741 atgacgaccg ggatgctgcg cagcgccggc gtctcgcgca ccgacttcag cacctccagg
     1801 ccgttgagct tgggcagctt caggtccagc agcatcacgg ccgggttgcc ttcggcgcga
     1861 ccagcgtgct cgccggcgcg gttcaggtag tcgagggcgg ccgcgccgtc gcgcacgacg
     1921 accacgtcgt tggccagctg gctgcgttcg agggctacga gggtcaattc caggtcacgg
     1981 gcgtcgtcct cgacgaggag gatgggtttg agcatgtgtt cagttctccg gggcgcgggg
     2041 cagggcgaat ccgaaggtcg ccccgcggcc gagctccccg gtcgcccata cctggccgcc
     2101 gtgtcgctcg acgatacggc ggatgctggc aagtcctatg cccgtgccct cgaattcgtc
     2161 cgattggtgc agtcgctgga agacgccgaa cagcttgccg acgtacttca tgtcgaagcc
     2221 gcagccattg tcctcgacct cgaggccctc ttccgggcgc cccgggctgc cgcgccgcac
     2281 cgggcggatg cggatgtgcg gcgaggcggc gtcgcgcgtg tacttgacgg cgttgctgac
     2341 caggttgcgc agcgccacct gcatcagcag cgggtcggcg tgcagcaccg gcaggtcggg
     2401 atcgacctcc cagcggatcg cgcgctccgg ttgcaggcgg gtgaactcgc gcaccagctc
     2461 cgcgaccagc acgcgcgtgt cgacgcggcc gcgcttgatc gcggcgcggc ccatgcgcga
     2521 gaagtcgagc aggccgtcga ccagctgccc ggagtaggcc gacgcgtcct tcacgtgcgc
     2581 gaggtagcgc ttcgcgcgct cgctgagcac cgcggaatcg acgtccagca cgagatccac
     2641 atagcccgag atgtggcgca tcggcgcgcg caggtcgtgc gagaccgtgt acgagaaggc
     2701 ctcgagctcc ttgttcgcgc gcccgagctc gccggcgagg tcggcgagct cttcggcgcg
     2761 gcgcagcacg atcgcgatca gcgactggcg cagttccagg gccgcggcga gctcggcggg
     2821 cgtccaaggc agcgcgcggt cgcgcagctc ctccttccag ctcgcgaagc tgcgccgcgg
     2881 atggatgcgg ccgtcggcgc gcagctcctt gttcggctcg ccggcccagc ggatcgtgcg
     2941 cgccaccgcc ggacggaacc acaggatcag gtggcggtgc acctgcgaga tcgacagcgc
     3001 aaggacgccg gcgcacgtat cgccccagcc ggcggcgtcg ggccagacct cgacgaggcg
     3061 gtcggtcgcg aagctgtcct cgccacgctc gacgatgcgg cgcccgagcg cgatcacgtg
     3121 ctcggcagcg ggcgtctcgc cgatcagccg gcacgactcg ttgagcacga cggccgcgcc
     3181 gccggcgcgc gcgaggtgga gcatcgcgtc gggtacgtcc agcaggccca tcagcgtgga
     3241 gtcgaggtcg gggcgctgcg cgatcagctg cagcgtgatg cggcgcagct cgaggcgctg
     3301 cgcgacttcg gcatgctcct cggcggcctc gacgcgcagg gacagcaggc ggccgaggtg
     3361 ctcgcaggcg gcgcgggtct cgaagtccag gtgccgcgcc tcgcggtggt ggcccgagac
     3421 cagcccccac agccggccct tggcgacgat cgagatcgac atcgacgcgc gcgtgcccat
     3481 gttgcgcatg tactcgaggt gaaccggcga gacgctgcgc aggccggcca gcgacaggtc
     3541 gatcgcgttc gcctgcacgc ccgggtccga cgacagcagc ggcacgggct cgtagtcggc
     3601 gtccgggatc acgcgcacgt agttcaggca gtagagcgcg cgcgcctggg gcgggatgtc
     3661 gctgcccggg aaatggtggc cggcgtagct cgcgtagccc gcgtccaggc tttcggcgat
     3721 cacctcgccg tggccctcgg cgtcgaagcg ataggcgagg cagcggccga agccggtcag
     3781 gcgcttgagc tcgcgggtgg cgagttcgca cagctcctcg accgtgcgcg cggcctgcag
     3841 cgcgggcacg acatggcgcg cgaggccgta gatcggcgag cgcgtgccgc tgccgtcgac
     3901 cgccggctcg aactcgacga tcgcgcggcc gccgcggcgg tgcacgctgg cgtcgaagcg
     3961 tccgccctcg agcgcgagcg tgccggcggg ggcgggcgac tcgtccgcgg gcca
//
LOCUS       DASVLN010000122         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_381921, whole genome shotgun sequence.
ACCESSION   DASVLN010000122 DASVLN010000000
VERSION     DASVLN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381921"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1558
                     /locus_tag="VF453_02670"
     CDS             248..1558
                     /locus_tag="VF453_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7686578.1"
                     /translation="MNALARLLLLASLLPVALAARAADELVIATVNNPHMLTMQKMTP
                     FFERANPDVHVRWVTLEEGQLRRAVTADVMSRGGRFDVTTIGMYEAPIWARRGWLEEI
                     RPDAAYDVDDLLPAIRDGLSWQGRLYAAPFYGESSMTLYRQDLAARAHLEMPLQPTWD
                     DIAKIAARLDDPKAGVHGICLRGKAGWGDNMALVSTMVNAYGGQWFDMAWHPRIDAPA
                     WREAVSRYVDLLRRHGPRDAVGNGFNENLALFEAGHCAVWVDATIAAALVTDPRRSQV
                     ADRVGFVQAPSARTSKGANWLWAWALAIPSDAVHKEQAQRFVRWATSRAYIELVGREV
                     GWSQVPTGTRISTYRNASFVGQARWAMAEAGAISLADPSDSTLPRSPYVGVQFAAIPE
                     FQAIGQATGEDIAAALAGRMSVDEALASAQAAADREMRHAGYYK"
BASE COUNT          214 a          619 c          580 g          207 t
ORIGIN      
        1 tcacgcgcga ggcccgcggc gccggcggcc ccgcgatccc gtatcgggtt ccgccgacgc
       61 agtacgtgcc ggcgcccccg gcctcgcgcg aagctccgtc gcgtggatgc gagccgggcc
      121 cgcggggcct ctcgcaccgc cgggcgagga ccggacgcat gcgtgcgcat ccgggggacg
      181 cgcttcgcgc ggccgtcggc gcttggacgc ctcgtcgcct gtccttccaa cgacgacagg
      241 agactccatg aacgcccttg cccgcctgct gctgctcgcc tccctgctgc ccgtggccct
      301 cgccgcccgg gccgcggacg agctggtcat cgccacggtg aacaacccgc acatgctcac
      361 catgcagaag atgacgccct tcttcgagcg cgccaacccc gacgtccacg tgcgctgggt
      421 gacgctggag gaagggcagc tgcggcgcgc cgtgacggcg gacgtgatgt cgcgcggcgg
      481 ccgcttcgac gtgacgacca tcggcatgta cgaggcgccg atctgggcgc gccgcggctg
      541 gctggaggag atccgcccgg acgcggccta cgacgtcgac gacctgctgc ccgcgatccg
      601 cgacgggctg tcctggcagg gaaggctgta cgccgcgccg ttctacggcg agagctcgat
      661 gaccctgtac cgccaggacc tggcggcccg cgcgcacctg gagatgccgc tgcagccgac
      721 ctgggacgac atcgccaaga tcgccgcccg cctcgacgac cccaaggccg gcgtgcacgg
      781 catctgcctg cgcggcaagg cgggctgggg cgacaacatg gcgctcgtct cgacgatggt
      841 caatgcgtat ggcggccagt ggttcgacat ggcctggcat ccgcgcatcg acgccccggc
      901 ctggcgcgag gcggtctcgc gctacgtgga cctgctgcgc cgccacggcc cgcgcgacgc
      961 ggtcggcaac ggcttcaacg agaacctcgc cctgttcgag gccggccact gcgcggtctg
     1021 ggtcgatgcc accatcgctg cggcgctcgt caccgacccg cgccgcagcc aggtcgcgga
     1081 ccgcgtcggc ttcgtgcagg cgccgtcggc gcgcaccagc aagggcgcga actggctctg
     1141 ggcctgggcg ctcgcgatcc cgtcggatgc cgtgcacaag gagcaggcgc agcgcttcgt
     1201 gcgctgggcg acctcgcggg cctacatcga gctcgtcggc cgcgaggtcg gctggagcca
     1261 ggtgcccacc ggcacgcgca tctcgaccta ccgcaacgcg agcttcgtcg gccaggcgcg
     1321 ctgggcgatg gccgaggccg gcgcgatctc gctcgccgac ccgtccgaca gcacgctgcc
     1381 gcgcagcccc tacgtcggcg tccagttcgc ggcgatcccc gagttccagg cgatcggcca
     1441 ggccaccggc gaggacatcg ccgccgcgct ggccggccgc atgagcgtcg acgaggcgct
     1501 cgcctccgcg caggccgccg ccgaccgcga gatgcggcat gccggctact acaagtgacc
     1561 ggccgcgggc cgggcgccgg aggtcggcag ccgtcggcag cgcgcggcac gatgcccgcc
//
LOCUS       DASVLN010000123         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_489570, whole genome shotgun sequence.
ACCESSION   DASVLN010000123 DASVLN010000000
VERSION     DASVLN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489570"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1328)
                     /locus_tag="VF453_02675"
     CDS             complement(165..1328)
                     /locus_tag="VF453_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7686579.1"
                     /translation="MKYLHLVWAGLFRRKARTILTLVSIVAAFLLFGMLDAVRLSFAQ
                     VGQSANGAQRLQTGSKLSFIQTLPRALGDQIAQVPGVKAVTFANWFGGAYQDPHNAVF
                     SFAVAPNYIDLYPEIAVNAAERKAFDETRTGALVGEKLAQRYHWKVGDRIPLQSTIFP
                     DREGSKNWQFDIVGILHSTDKKANGFFDQMFLLNWKYFDDTTPYNRGQVGWYVTRVAD
                     VNQADAVARRIDALSANSDHETKTQTEQAATANWMKQLADIGLIVGSIMGAVFFTLLL
                     LSGNTMMQAVRERTAELAVMKTIGFRDGSVLAMVLAESVLLLVIGGVLGLVVASGLVP
                     VVTAASGGVLNLPPVGGSTWGLGIGLMALFGLLVGAVPAASAMRLNIVDALRR"
     gene            complement(1339..2025)
                     /locus_tag="VF453_02680"
     CDS             complement(1339..2025)
                     /locus_tag="VF453_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7686580.1"
                     /translation="MPTLIEIRDVSKVYMRGKQTVEVLHHVNLDVAQGDFLALMGPSG
                     SGKTTLLNLIGGLDQPSGGSITVGGERIDQLGASALARWRASKVGFVFQFYNLMPTLS
                     AQRNVELPLLLTKLSGAERRKRASIALQLVGLSDRASHKPTELSGGQQQRVSIARAIV
                     SDPQLLVCDEPTGDLDRQSAEDVLGLLRALNRDHGKTILMVTHDPKAAEYANSTLELD
                     KGTLVERQPA"
     gene            complement(2103..>2636)
                     /locus_tag="VF453_02685"
     CDS             complement(2103..>2636)
                     /locus_tag="VF453_02685"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7686581.1"
                     /translation="FTRTGVGTIVDMDSLEVEVDVAEAYIGHVSAGMRCEAVLDAYPD
                     WKIPAHVIAVIPEADRGKATVKVRVALEQKDARLVPDIGVRVSFLASKQPAAATPHGV
                     LVPPGALAERDGRTVVFVVREGHAHAQALPGGARDAGGMKLVSDGLEPGDVVVVSPPD
                     GLADGAAVKPTDAAAAH"
BASE COUNT          381 a          916 c          945 g          394 t
ORIGIN      
        1 aggccgcctg ccgcccgcgc cgcgtcagcg cgagccaggc cgcgacgagc gccgccaccg
       61 cgagcgcgcc cggccagggc agcagcaccc acacggcgag caccgcgacg atcagcagca
      121 cgagcccggt gtttcgaaga aaggccatgg cgtcgctccc ctgttcagcg ccgcagcgcg
      181 tcgacgatgt tcagccgcat cgcgctcgct gccggcacgg cgccgaccag caggccgaac
      241 agcgccatca gcccgatccc gaggccccag gtgctgccgc ccaccggcgg caggttcagc
      301 acgccgccgc tggccgccgt cacgacaggc accagccccg acgccaccac gagtcccagc
      361 accccgccga tcaccagcag cagcacggac tcggcgagca ccatcgcgag cacgctgccg
      421 tcgcgaaagc cgatcgtctt catgacggcg agctccgcgg tgcgctcgcg cacggcctgc
      481 atcatcgtgt tgcccgagag cagcagcagc gtgaagaaca cggcgcccat gatggagccg
      541 acgatgaggc cgatgtcggc cagctgcttc atccagttgg cggtcgcggc ctgctcggtc
      601 tgcgtcttcg tctcgtggtc cgaattagcg gagagcgcgt cgatgcggcg cgcaacggcg
      661 tcggcctggt tcacgtcggc cacccgcgtc acgtaccagc cgacctggcc gcggttgtag
      721 ggcgtcgtgt cgtcgaagta cttccagttc agcaggaaca tctggtcgaa gaagccgttc
      781 gccttcttgt cggtcgaatg caggatgccg acgatgtcga actgccagtt cttgctcccc
      841 tcgcggtcgg ggaagatcgt cgactgcagc gggatgcggt cgccgacctt ccagtggtag
      901 cgctgggcga gcttctcgcc gaccagcgcc ccggtgcgcg tctcgtcgaa ggccttgcgc
      961 tcggcagcgt tgacggcgat ctccgggtag aggtcgatgt agttgggtgc gaccgcgaag
     1021 ctgaagaccg cgttgtgcgg atcctggtag gcgccgccga accagttggc gaacgtgacc
     1081 gccttgacgc cgggcacctg cgcgatctgg tcgccgagcg cgcgcggcag ggtctggatg
     1141 aaggacagct tcgagcccgt ctgcaggcgc tgcgcgccgt tggcgctctg gccgacctgc
     1201 gcgaacgaca gccgcaccgc atccagcatc ccgaacagca ggaaggccgc gacgatggac
     1261 acgagcgtca ggatcgtccg cgccttgcgc cggaacaggc cggcccatac caggtggagg
     1321 tatttcatcg cgcgctcctc aggccggctg gcgctcgacc agcgtgccct tgtcgagctc
     1381 cagcgtgctg ttcgcgtact cggcggcctt gggatcgtgg gtgaccatca ggatcgtctt
     1441 gccgtggtcg cggttgagcg cgcgcagcag gccgagcacg tcctcggccg actggcggtc
     1501 caggtcgccg gtcggctcgt cgcagaccag cagctgcggg tcggagacga tggcgcgcgc
     1561 gatcgagacg cgctgctgct ggccgcccga gagctcggtc ggcttgtggc tcgcgcggtc
     1621 cgacaggccg acgagctgca gcgcgatcga ggcgcgcttg cggcgttccg cccccgagag
     1681 cttcgtgagc agcagtggca gttccacgtt gcgctgcgcc gacagcgtgg gcatcaggtt
     1741 gtagaactgg aacacgaagc cgaccttgga ggcgcgccag cgcgcgagcg ccgaggctcc
     1801 gagctggtcg atgcgctcgc cgcccacggt gatgctgccg ccgctgggct ggtccaggcc
     1861 gccgatcagg ttcagcagcg tcgtcttgcc cgagcccgag ggccccatga gcgcgaggaa
     1921 gtcgccctgc gcgacgtcga ggttcacgtg gtgcaggacc tcgacggtct gcttgccgcg
     1981 catgtagacc ttcgagacgt cgcggatttc gatcagcgtg ggcatgcgtg ggctcctgca
     2041 gtcgtcggcg cgttcgcgcg ccggggtggc gatcggggac tcggggttcg gggatcggtc
     2101 gctcagtgcg cggcggccgc gtcggtgggc ttgacggcgg cgccgtcggc gagcccgtcc
     2161 ggcggcgaca cgacgacgac gtcgccgggc tcgagaccgt cggataccag cttcatcccg
     2221 ccggcgtcgc gcgcgccgcc ggggagtgcc tgcgcgtgcg cgtggccctc gcgcacgacg
     2281 aagacgacgg tgcggccgtc gcgttcggcg agcgcgcccg gcggcaccag cacgccgtgc
     2341 ggcgtcgcgg ccgcgggctg cttcgacgcg aggaacgaga cgcgcacgcc gatgtcgggc
     2401 accagccgcg cgtccttctg ctccaacgcc acgcgcacct tgacggtcgc cttgccgcgg
     2461 tcggcctcgg ggatgacggc gatgacgtgg gccgggatct tccagtcagg gtaggcgtcc
     2521 agcacggcct cgcagcgcat gccggcgctg acatggccga tgtaggcctc ggcgacgtcg
     2581 acctcgacct cgagcgagtc catgtcgacg atcgtgccga cgccggtgcg cgtgaa
//
LOCUS       DASVLN010000124         6399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_328382, whole genome shotgun sequence.
ACCESSION   DASVLN010000124 DASVLN010000000
VERSION     DASVLN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328382"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..236
                     /locus_tag="VF453_02690"
     tRNA            161..236
                     /locus_tag="VF453_02690"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:194..196,aa:Thr,seq:tgt)
     gene            1222..1923
                     /locus_tag="VF453_02695"
     CDS             1222..1923
                     /locus_tag="VF453_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7686582.1"
                     /translation="MLYSRTQATRRPARSKRPATPGALLLAAAIALPGASALAAPVVY
                     TGYDNHVSTLSLNAVVAAETFYNAAAPRPSIIDFESPLPAGVSITGDGEQVIDTDCGA
                     GCGFNTSPGGTHFLQLAGTGANVTFTFSTPVTAFGFYVTGLQSSVLTGERVTFDDGSA
                     QFIPVAPVTNGDAFIGFIDLAGVTSVTYEATLDVVGIDDVWYGSQLSAVPEPATFAMV
                     GAGLIGVGLARRRRA"
     gene            complement(2065..2394)
                     /locus_tag="VF453_02700"
     CDS             complement(2065..2394)
                     /locus_tag="VF453_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686583.1"
                     /translation="MVVIDKLDGRKRIAGDHYGDPEAGLITPDEQWFVSVGDGVQCLR
                     ADGRFVTFFHQGRPPLDGMPGEVAWSVTAVDLTAGHTLRIIVDPNSDEASEWLIDLDA
                     EVVTRVG"
     gene            complement(2460..3416)
                     /locus_tag="VF453_02705"
     CDS             complement(2460..3416)
                     /locus_tag="VF453_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7686584.1"
                     /translation="MDRFTRIGVFVAAVDDGSFAAAARRLGMSAAMAGKYVSALEAEL
                     GVRLLQRTTRSLTLTEAGRRYHARCKRLIEAFEEANREATDSRHVAQGTLRVAAPVAF
                     GALHLGPVVARYLADNPRVDVQVLMDDGYADLLAAGIDVAIRIGRLEDSSLVARRIAP
                     CRMTLCASPGYLERHGHPHAIDELLHADRLTFSQAVSAGDWTLVAPDGRARVVDGPSR
                     LSANNMQMLSAAARAGVGIAYGPTFVFGDDIARGELVELLPDHRASDLAIHAVYPSAR
                     HVPLKVRLFVDRLAADFGDVPPWDRFRGAGVARPASSKPNDQ"
     gene            3577..4725
                     /locus_tag="VF453_02710"
     CDS             3577..4725
                     /locus_tag="VF453_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7686585.1"
                     /translation="MDIQSNGVRIHVTQQGDGDLALVFLHYYGGSSRTWRFVMDALSR
                     RYRTVATDHRGWGRSQAPEAGYRIEDLADDAQGVIEALGLRRYVLVGHSMGGKTAQLL
                     ASRRPPGLEGLVLVAPSPPSPMQLSDEQRAVMAGAYASRESVEWVVDHVLTARPLSGE
                     VREQVVADSLSGAPQARLGWPNVAMRDDITREVSAIDVPVLVVSGDRDQVDRVETLRE
                     ELLPRIPGARLQVLPGVGHLSPLEAPSELAAAIADFVAALEGSLRTPGQLVAAFDRAF
                     NAGDVDGLVELFAANATMRMTDGTVVSGRDALRRSFQALLFTRPRITNRMRAAYASDD
                     LALALAEWTVAATMPDGSAVSNSGVATQVLERGADRAWRLRISNPLGT"
     gene            4858..>6399
                     /locus_tag="VF453_02715"
     CDS             4858..>6399
                     /locus_tag="VF453_02715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7686586.1"
                     /translation="MSLAALAPEWPVLNALLDEALALSPGARQAWLRSLERDAPGRHD
                     AVRRLLALHERMDDDFLATLPPLPAAAFGEPGAAAGARVGPYRLVEEIGQGGMSTVWR
                     AAHVDDGGRPAVALKLPRLTWGGRFAERLDRERAIVAGLEHPHIARFVDAGLDEAGRP
                     WLATELVEGEPIDAHCARHASSLQARLELLLQVCDAVAHAHSRLVIHRDLKPANILVS
                     PRGEVKLLDFGIAKLLEDGQARETALTQAAGRALTPRFASPEQIRGGPLGTACDVYAI
                     GVVAFLLLAGDYPHALRRGTAAELEDAIAHAPPRRASDVAREPGWRRQLRGDLDAILA
                     KALSAEIAGRYPGADALARDLRRHLAGLPIEARPHSRLYVAQRFVHRHRAAVGMLAAI
                     VVALGSGLGAAAWQASLATQQRRLAERSLERERAVKEMLVEILSVAVTADPARLREPD
                     GFGVLLQEKFDQLEHRFRGRPDEWLDLLEVVSTRLPDYGDYMCSYAVGQRYLALLQAT
                     HANPLR"
BASE COUNT          907 a         2265 c         2284 g          943 t
ORIGIN      
        1 ccgacgacca cctccgcgac ggacgggcag gacgggccgg ccgcggtggc cgaggacgcc
       61 cccgagccgc ccgaggctcc gacccccccg cccagcggcc cgcgcccggc cctgaagcgc
      121 atcaagtaaa gtctcctgcc ggcccgcctc gggccggact gccgacttag ctcagctggt
      181 agagcatccg ccttgtaagc gggaggtcat ccgttcgatt cggatagtcg gcaccaaacc
      241 cttgccttgc gacacggcga tacccgtcga tggaaggctg ccagcgcccg tatgggttcg
      301 gccgccgcgc cgtgacacca ggcgcttctt cgcgctgacg ggtgtccgcc gcggattcac
      361 gcggtgaagg tgccgcttga gatgaggctg ggcgcggctg cgacgatggt cttggatcac
      421 tgagtcgccc agcggcgggg tcagtcccga cttcgagtcg gtgccccacg tcaaggcgat
      481 attcgacgcg ccgggactgg acatcgaata caaggtgcac ctgctgtccg acacgctgat
      541 tgcacagctc cgggaagtct gacccgtggc ggtcgtcgtc ggtggcaccg cggtcggtgt
      601 ctacgaactc gtggtcggcg cggcggcagc gctcacggcg ctgttcctcg tctcccctac
      661 aggccagaag gtggcacgcg acgctgcgaa ggcgacttcg gacgccctcg agcgtgcacg
      721 caggcggccc gacgaggatc cggagccgaa gcctcccaag acgaccgtca cgccgtgccc
      781 gtgcgatccg gatggcaagg gcccggagcc gtgcccgacc tgcggaaagg gaatcagccc
      841 ggtgaagggt taccgacctc gctatctcga tccacccagc gcgccgcaga tggacgaggt
      901 gtacgagcgc ggcagcgcga ggatgaggca gagcgggcgt gcgtggtgtc catggcgaag
      961 tcgacgcggc aatgcgcgac gcggcggctg cattgtccag ggccggccgc gtcatcctgc
     1021 aggtcgatcc gctgcagccg atggcgggcg gatggtccga cgcggtggcg gctgcatcgt
     1081 gcggatggcc cctggacgag gcgcaggccc ggatgtccgg aaatccgatg cccgcacgta
     1141 catcaagtgg gcagacagcc cgcctccggc cgcgcggacg acgagtggtc ggcgcaacca
     1201 tcgaacatcc aggagtcccg catgctgtat tcccgcacac aagcgacccg caggcccgcg
     1261 cggtcgaagc gccccgccac gccgggcgcg ctgctgttgg cggccgccat cgcgcttccc
     1321 ggcgcgagcg ccttggcggc acccgtggtc tacaccggat acgacaacca cgtctccacg
     1381 ctcagcctga acgccgtcgt cgccgccgag acgttctaca acgccgcggc cccgcggccc
     1441 tccatcatcg acttcgagtc gccgctgccc gccggcgtca gcatcacggg ggatggcgag
     1501 caggtcatcg ataccgactg cggcgccgga tgcggcttca acaccagccc cggcggcacg
     1561 cacttcctgc agctggccgg gaccggcgcc aacgtcacgt tcaccttttc gacgccggtg
     1621 accgcattcg gcttctacgt cacgggcctg cagagcagcg tcctgaccgg cgagcgggtg
     1681 accttcgacg acggctccgc gcagttcatc ccggtggcgc cggtgacgaa cggcgatgcc
     1741 ttcatcggct tcatcgatct cgcgggcgtc accagcgtca cctatgaggc caccctggac
     1801 gtggtcggca tcgacgacgt ctggtacggc agccagttgt ccgcggtgcc cgagcccgcg
     1861 acgttcgcga tggtcggtgc cggactgatc ggcgtcggcc ttgcgcgccg ccggcgcgcg
     1921 tgaacctgcg cgggaggccg actgacgagg catcgacgca agggttcgcg ctggcctccc
     1981 ggcgcaggag gagctgcccg caggacatga ccttcctgcg cgtggaatcg agcggcgccg
     2041 aaatcgtctt cgcacggcga aggctcaacc cactctcgtc acgacctctg cgtcgaggtc
     2101 gatcagccac tcggacgcct catcgctgtt cgggtcgacg atgatccgca gggtatggcc
     2161 ggcagtcagg tcgaccgcag tcaccgacca tgcgacttcg ccgggcatgc cgtccaaagg
     2221 gggacggccc tgatggaaga acgtcacgaa tcgaccgtcc gcacggaggc actggacgcc
     2281 gtcgccgacg ctgacgaacc attgctcgtc gggcgttatg aggccagcct ccggatcgcc
     2341 gtagtgatct ccggcgatcc gcttcctgcc atcgagtttg tctatgacga ccacggattc
     2401 gtagctgtgt gcgagtatga aattcgcaga ctcggcgaga gtccgaagcg tcgaggcagt
     2461 cattggtcgt tcggcttgga agaggcgggt ctcgctaccc ctgcgccccg gaatcggtcc
     2521 catggcggaa cgtcaccgaa gtccgccgca aggcggtcga cgaacaggcg caccttgagc
     2581 ggcacgtggc gcgcgctggg gtacaccgca tggatcgcga ggtccgaggc gcggtggtcc
     2641 ggcaggagct cgacgagctc gccgcgcgcg atgtcgtcgc cgaacacgaa cgtcggcccg
     2701 taggcaatgc cgacgcccgc gcgcgcggcc gcggacagca tctgcatgtt gttggccgac
     2761 aggcgcgacg gcccgtcgac gacgcgggcg cggccgtccg gcgcgaccag cgtccagtcg
     2821 cccgccgaga cggcctggct gaacgtcagc cggtcggcgt gcaggagctc gtcgatggcg
     2881 tggggatggc cgtggcgctc gagatagccg ggtgacgcgc agagggtcat gcgacagggg
     2941 gcgatccgtc tcgcgaccag cgacgagtcc tcgagccggc cgatccggat cgcgacgtcg
     3001 atgcccgccg cgagcaggtc ggcgtagccg tcgtccatca gcacctgcac gtcgacgcgc
     3061 gggttgtccg cgaggtagcg ggccaccacg ggcccgagat gcagcgcccc gaaggccacc
     3121 ggcgcggcca cgcgcaacgt gccctgcgcg acgtgccggg agtcggtcgc ttcgcggttc
     3181 gcctcctcga aggcctcgat gaggcgcttg cagcgtgcgt ggtagcgccg tccggcctcg
     3241 gtcagcgtca ggctgcgggt cgtcctctgg agcaggcgga cccccagctc cgcctcgagc
     3301 gcgctcacgt acttgcccgc catggccgcc gacatgccca ggcgccgggc ggccgcggcg
     3361 aagctgccgt cgtcgacggc ggccacgaag acgccgatgc gggtgaagcg gtccacgaac
     3421 tgtccttgat tcgatcgcta tgagatcgac tgtatttctc ggttgcgaga ttatcaatga
     3481 gcgtgatcga ctctaggatt ctccgcaggg tcgggtggcg tctcgcgacg cgggccggca
     3541 aacgggcgcg agcgcccctt caaggagaac aggcagatgg acatccaatc gaacggcgtg
     3601 cgcatccacg tcacgcagca gggcgacggc gatctcgcgc tcgtcttcct ccactattac
     3661 ggcggctcgt cgcgcacctg gcgtttcgtc atggacgcgc tgtcgcggcg gtatcgcacc
     3721 gtggcgaccg accaccgtgg ctggggccgg tcgcaggcgc cggaggccgg ctatcgcatc
     3781 gaggatctcg cggacgacgc gcagggcgtc atcgaggcgc tgggcctgcg gcgctacgtg
     3841 ctcgtcgggc actcgatggg cggcaagacg gcccaattgc tcgcctcgcg tcggccgccg
     3901 ggcctggagg ggctcgtcct ggtcgcgccg tcgccgccgt cgcccatgca gctgtccgac
     3961 gagcagcgcg ccgtcatggc cggtgcctac gcgtcgcgcg aatcggtcga gtgggtcgtc
     4021 gaccacgtgc tgaccgccag gccgctgtcc ggcgaggtgc gcgagcaggt cgtcgccgac
     4081 agcctgtccg gcgcgccgca ggccaggctc ggctggccga acgtcgcgat gcgggacgac
     4141 atcacgcgcg aggtgtcggc gatcgacgtg ccggtgctgg tcgtctcggg cgaccgcgat
     4201 caggtcgatc gcgtcgaaac cctgcgcgag gaactgctgc cgcgcattcc cggcgcgcgc
     4261 ctgcaggtgc tgcccggggt cggccacctg tcgcccctcg aggcgccctc ggagctcgcc
     4321 gcggccatcg ccgacttcgt cgcggcgctg gagggcagtc tgcgcacgcc cgggcagctg
     4381 gtggccgcgt tcgaccgcgc cttcaatgcg ggggacgtcg acgggctcgt cgagctgttc
     4441 gcggccaacg cgacgatgcg gatgaccgac ggcaccgtcg tgagcggccg cgacgcgttg
     4501 cggcgatcct tccaggccct gttgttcacg cgcccgcgga tcaccaaccg gatgcgcgcc
     4561 gcctatgcga gcgacgacct cgccttggcg ctcgccgagt ggacggtcgc cgcgacgatg
     4621 cctgacggca gcgcggtgag caactcgggc gtcgcgaccc aggtgctcga gcgtggtgcg
     4681 gaccgtgcct ggcggctgcg catctccaat ccgctcggca cgtgaagcgg cgcgacgatc
     4741 cgacgcttgt gaaatcagct gcaatccgga tgtcgccgcc ggccgcggca ccggctcggg
     4801 catggcttcg gcgtcgggga tggagtaagg tcgcgcgtcg ccgctgccgc cgtcatcatg
     4861 tccctcgccg cgctcgcccc ggaatggccg gtcctcaacg ccctcctcga cgaggccctg
     4921 gccttgtcgc ccggcgcgcg ccaggcatgg ctgcgttcgc tggagcggga cgcccccgga
     4981 cgacatgacg cggtgcgtcg cctgctcgcg ctgcacgagc gcatggacga cgacttcctg
     5041 gccacgcttc cgccgctgcc ggcggcggcg ttcggcgagc cgggggccgc cgccggcgcg
     5101 cgtgtcggcc cgtaccggct cgtggaggag atcggccagg gcggcatgag caccgtctgg
     5161 cgcgccgcgc acgtcgacga cggcggccgg ccggcggtcg cgttgaagct tcctcggctg
     5221 acctggggcg gccgcttcgc cgagcgcctg gatcgcgaac gcgcgatcgt ggcgggcctc
     5281 gagcatccgc acatcgcgcg cttcgtcgat gcggggctgg acgaggcggg ccggccgtgg
     5341 ctggccacgg agctggtcga gggcgaaccg atcgatgccc attgcgcgcg gcacgcgtcg
     5401 tcgctgcagg cgcgcctcga gctgctgctc caggtctgcg acgcggtcgc gcacgcgcac
     5461 tcgaggctcg tgatccaccg cgacctcaag ccggccaaca tcctcgtgtc gccgcgcggc
     5521 gaggtgaagc tcctcgattt cggcatcgcc aagctgctgg aggacgggca ggcgcgggag
     5581 acggccctca cccaggcggc cggacgtgcg ctcacgccgc ggttcgccag tcccgagcag
     5641 atccggggcg gccccctcgg cacggcgtgc gacgtctatg cgatcggcgt cgtcgccttc
     5701 ctgctgctcg cgggcgacta tccccatgcc ctgcggcgcg gcacggccgc ggagctggag
     5761 gacgccatcg cccacgcgcc gccccgccgg gccagcgacg tggcgcgcga gcccggatgg
     5821 cgtcgccagc tgcggggcga cctcgacgcg atcctcgcga aggccctgag cgcggagatc
     5881 gccggccgct atccgggcgc cgacgcgctc gcacgggacc tgcgtcggca tctcgcgggc
     5941 ctgccgatcg aggcccggcc gcacagccgg ctctacgtcg cgcagcgctt cgtgcaccgt
     6001 caccgcgccg cggtgggcat gctggcggcg atcgtcgtcg ccctgggcag cgggctgggc
     6061 gcggcggcct ggcaggcctc cctggccacc cagcagcgcc gccttgcgga gcgatcgctg
     6121 gaacgcgagc gtgccgtcaa ggagatgctg gtggagatcc tctcggtcgc ggtgacggcc
     6181 gatccggcgc gcctgcgcga gcccgacggc ttcggcgtgc tgctgcagga gaagttcgac
     6241 cagctcgagc accgcttccg cggtcgcccc gacgaatggc tcgacctgct cgaggtcgtc
     6301 tccacgcgcc tgccggacta cggcgactac atgtgttcgt atgcggtcgg ccagcgctac
     6361 ctggcgctct tgcaggcgac gcacgccaac ccgctgcgc
//
LOCUS       DASVLN010000125         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_489697, whole genome shotgun sequence.
ACCESSION   DASVLN010000125 DASVLN010000000
VERSION     DASVLN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489697"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VF453_02720"
     CDS             <1..373
                     /locus_tag="VF453_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7686587.1"
                     /translation="GFAVVASEVRSLAGRSAAAAREIGQLISHSVGQVAEGSRLVDEA
                     GRTMRDIVASTHRVSALVDVISTASAEQSAGVAQVSLEVSRMDRSTQENAALVEQGAA
                     AAANLKAKAHELVASVAAFRL"
     gene            603..1091
                     /locus_tag="VF453_02725"
     CDS             603..1091
                     /locus_tag="VF453_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7686588.1"
                     /translation="MDLIDTLFGSGSELDALQMALRAVAVSILTIAMIRLAGRRAFGQ
                     HRPFDTCLAVLLGAVLSRAVVGASPFWATMAAGLAIVVVHRLVAYASMLWPAVDDFVS
                     GDLRVLVREGRPDEAQMRKALVTDRDLAEAVRKRTGDESVPVECAVLERDGKLTVKLR
                     DG"
     gene            complement(1130..>3350)
                     /locus_tag="VF453_02730"
     CDS             complement(1130..>3350)
                     /locus_tag="VF453_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7686589.1"
                     /translation="AGVGGDDAGEKWLSWTHHALRDLHGKVFAIHSVGRDVTERRATE
                     HSLRASKAKLQKLYEATPAMLHSMDAQGVLVTVSDVWLQRLGYGRDEVIGRRSREFFA
                     PEFVARAAQDLTTALLRDGRVDEVRGQMLRKDGSRMDVLFSMIVERRDDGRPDHFLAV
                     VEDVTERIRIARELADKHELLRVTLESIGDAVVTTDARQRVEWLNPVAERMTGWLGSQ
                     ARGRPVAQVVHVVDHDTRAPAPSTTARCLAQGRTADGADNAVLVARDGAEYAIEASAS
                     PMRASDGALLGAVLVFRDVSQQRRLSQEMSYRARHDELTGLLNRSEFEQHLDRLLADA
                     PAAGQAHSLLYIDLDQFKLVNDACGHAAGDRLLKQIAAMLQSCVRQRDTIARLGGDEF
                     GIVLERCGTEQARRIADQICSQMEEFRFVHDDRRFRLGTSIGLVPLDERWHGHDAPLQ
                     AADSAAYAAKEAGRNRVHEWFDTDQAMRARMGQTQWGSRLELALDEDRFELFGQRIAP
                     LASNDGDGRVHVEVLLRLRDTNGALIAPGAFLPAAERFHVASRIDRWVVRHVFEWIAA
                     HDDLGGVDAFAINLSGQSMGDRAFHRYVTDMVAATKVDARRLCFEITETAAITNMADA
                     AAFVEAMRAIGARIALDDFGAGASSFGYLKSLPVDYLKIDGQFVRDLNEDALDLAAVR
                     CFTEVARVIGVRTVAEFVESDALLATLRDIGVDYVQGYTVHKPEPLALAARGAAEIA"
BASE COUNT          439 a         1177 c         1273 g          461 t
ORIGIN      
        1 cggcttcgcg gtggtggcga gcgaggtccg cagcctcgcg ggccgcagcg ccgcggcggc
       61 gcgcgagatc ggccagctga tatcgcacag tgtcggccag gtggccgagg gctcgcgcct
      121 ggtcgacgag gcgggccgca cgatgcgcga catcgtggcc tcgacgcatc gcgtcagcgc
      181 gctcgtggac gtcatcagca ccgcgagcgc ggagcagagc gcgggcgtcg cgcaggtgag
      241 cctcgaggtc tcgcgcatgg accgctcgac gcaggagaac gcggcgctcg tcgagcaggg
      301 cgccgcggcc gccgcgaacc tgaaggcgaa ggcgcacgag ctcgtggcct cggtggctgc
      361 gttccggctc tgaagcgcgc gcggcgcgcg tgcccggtgg ccgcctgtcc tacgcgaaca
      421 ggcggacgcg gccgacgcgg gtcgggccgc cgcgaaggca cagtgagcac cagcgcagtc
      481 ggcgggaaga actcgaacaa cgcccgcccg ccgcgccact tttcgcactt ttcgcagtcg
      541 tgcaggaggg aacggccggc ggccgcggcg tcgatccgcg ggcggcgccg gcctcgtgcg
      601 agatggacct catcgacacc ctgttcggct ccggctccga gctcgacgcg ctgcagatgg
      661 ccctgcgcgc ggtcgccgtc tcgatcctca ccatcgcgat gatccgcctg gccggtcggc
      721 gcgcgttcgg gcagcaccgg ccgttcgaca cctgcctggc cgtgctgctg ggcgcggtgc
      781 tgagccgcgc cgtcgtcggc gcgtcgccgt tctgggcgac gatggccgcg ggcctggcga
      841 tcgtggtcgt gcaccgcctc gtcgcctacg cgagcatgct ctggccggcc gtcgacgact
      901 tcgtgagcgg tgacctgcgc gtgctggtgc gcgagggccg gccggacgag gcgcagatgc
      961 gcaaggcgct cgtcaccgac cgcgacctcg ccgaggcggt gcgcaagcgc acgggcgacg
     1021 agtccgtgcc ggtggaatgc gcggtgctcg agcgcgacgg caagctgacc gtcaagctgc
     1081 gggacggctg aaggagacgg ccgcggcccg gcgcccgccg ggtcgcggat caggcgatct
     1141 cggcggctcc gcgcgcggcc agggcgagcg gctcgggctt gtgcaccgta tacccctgca
     1201 cgtagtcgac gccgatgtcg cgcagcgtcg cgagcagggc gtcggactcg acgaattcgg
     1261 ccaccgtgcg cacgccgatg acgcgggcga cctcggtgaa gcagcgcacc gcggcgaggt
     1321 ccagggcgtc ctcgttgagg tcgcgcacga actggccgtc gatcttcagg tagtccaccg
     1381 gcagcgactt caggtagccg aacgacgatg cgcccgcgcc gaagtcgtcg agggcgatgc
     1441 gggcgccgat cgcgcgcatc gcctcgacga aggccgcggc gtcggccatg ttggtgatcg
     1501 cggcggtctc cgtgatctcg aagcacaggc gccgcgcatc gaccttcgtg gccgcgacca
     1561 tgtcggtgac gtagcggtgg aacgcgcggt cgcccatcga ctggcccgac aggttgatcg
     1621 cgaacgcgtc gacgccgccg aggtcgtcgt gggccgcgat ccactcgaag acgtggcgca
     1681 ccacccagcg gtcgatgcgc gaggcgacgt ggaagcgctc ggccgccggc aggaacgcgc
     1741 cgggcgcgat gagggcgccg ttggtgtcgc gcaggcgcag cagcacctcg acatggacgc
     1801 gcccgtcgcc gtcgttcgac gcgagcggcg cgatgcgctg gccgaacagc tcgaagcggt
     1861 cctcgtcgag cgccagctcg aggcggctgc cccactgcgt ctggcccatg cgcgcgcgca
     1921 tcgcctggtc ggtgtcgaac cactcgtgca cgcggttgcg gccggcctcc ttggccgcgt
     1981 aggcggcgct gtcggcggcc tgcagcggcg cgtcgtggcc atgccagcgc tcgtccagcg
     2041 gcacgagccc gatgctcgtg cccaggcgga agcggcggtc gtcgtggacg aagcggaact
     2101 cctccatctg cgagcagatc tggtcggcga tgcgccgcgc ctgctcggtg ccgcagcgct
     2161 ccaggacgat gccgaactcg tcgccgccca ggcgcgcgat cgtgtcgcgc tggcgcacgc
     2221 acgactgcag catcgccgcg atctgcttga gcaggcggtc gcccgcggcg tggccgcagg
     2281 cgtcgttgac gagcttgaac tggtcgaggt cgatgtacag cagcgagtgc gcctggccgg
     2341 cggcgggtgc gtcggcgagc aggcggtcca ggtgctgctc gaactcggag cgattgagca
     2401 gcccggtgag ctcgtcgtgc ctggcgcggt acgacatctc ctggctgagc cggcgctgct
     2461 gcgacacgtc gcggaacacc aggacggcgc ccagcagcgc gccgtcgctc gcgcgcatcg
     2521 gcgaggccga ggcctcgatc gcgtactccg cgccgtcgcg cgcgacgagc accgcgttgt
     2581 ccgcgccgtc ggccgtgcgg ccctgggcga ggcagcgggc cgtggtgctc ggagcgggcg
     2641 cacgcgtgtc gtggtcgacg acgtggacga cctgcgcgac cggccggccg cgtgcttgcg
     2701 agccgagcca gccggtcatg cgctcggcca ccgggttgag ccactcgacg cgctggcggg
     2761 cgtcggtcgt gacgacggcg tcgccgatcg actccagcgt gacgcgcagc agctcgtgct
     2821 tgtcggcgag ctcgcgcgcg atgcggatgc gctcggtgac gtcctcgacg accgccagga
     2881 aatggtcggg ccggccgtcg tcgcggcgct cgacgatcat cgagaacagc acgtccatgc
     2941 ggctgccgtc cttgcgcagc atctggccgc gcacctcgtc gacgcggccg tcgcgcagca
     3001 gcgccgtggt caagtcctgg gcggcgcggg cgacgaactc cggcgcgaag aactcgcgcg
     3061 atcgccggcc gatcacctcg tcgcgcccgt agccgaggcg ctgcagccag acgtcgctga
     3121 cggtcacgag cacgccctgc gcatccatgc tgtgcagcat cgcgggcgtc gcctcgtaga
     3181 gcttctgcag cttggccttg ctcgcgcgca gggagtgctc ggtggcgcgg cgctcggtga
     3241 cgtcgcggcc caccgagtgg atcgcgaaga ccttgccgtg gaggtcgcgc agcgcgtgat
     3301 gcgtccagga cagccacttc tcgccggcgt cgtcgccgcc gacgccggcg
//
LOCUS       DASVLN010000126         5857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_168156, whole genome shotgun sequence.
ACCESSION   DASVLN010000126 DASVLN010000000
VERSION     DASVLN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168156"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..2451
                     /gene="lon"
                     /locus_tag="VF453_02735"
     CDS             124..2451
                     /gene="lon"
                     /locus_tag="VF453_02735"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7686590.1"
                     /translation="MSEAAPVDLPEDVVALVPMRDVVLFPHVMMPVGVGRAKSIAALL
                     QAVQSARPLGIVLQKDPHADEPGRAELCDVGTLAQVVKHVPGEGVQHAIVHGLSRLRI
                     VSLVEGYPFLAARIERLPEDATPPDTQTEALALQLRERAAEILGLLPGVPAEAAHMMQ
                     AARAPQELADIVASLVDADPAEKQALLESAVLADRLRSLLSLVARRIQVLRLSQEIGE
                     RTRQQLDDRQRRFLLKEQLRTIQQELGEDGETSAELERLQKLVADAGMPPEIDERARR
                     ELARLQRLQDSPGEAAVLRTWLEWMTELPWRTPAPLEIDIERARLELSRQHFGLEPVK
                     RRILEFLAVRKLNPSAPAPILCFVGPPGVGKTSLGQCIARTLQRPFVRVSLGGVHDEA
                     EVRGHRRTYVGALPGNIVQALRKAGTLDPVLMLDEMDKLSASAHGDPSAALLEVLDPE
                     QNASFRDNYLGVPVDLSRVTFLCTANVLEQMPAPVRDRVEVIELPGYSLEEKERIARA
                     HLLPRQLAAHGLQATAFELDDAALRRLASDYTREAGVRQLDRCIGSVARHAAMAVVES
                     PGTRVHVTERDLAAILGPAPFEHEAAMRRSVAGIATGLAWTPAGGDILFVEAARMPGD
                     GQLILTGQLGDVMKESARAALTLVRSRAAGLDVAPDAFAKADIHVHVPAGAVPKDGPS
                     AGVAMFAALASVFTGRPVRAQVAMTGEISLRGLVLPIGGVREKVLAAQRAGVATVLLP
                     RRNEKDLADMPPDTRAGLRVVLVDSVDDVLREGLE"
     gene            2676..3179
                     /locus_tag="VF453_02740"
     CDS             2676..3179
                     /locus_tag="VF453_02740"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HEX7686591.1"
                     /translation="MPEPTSRSARPAPAAHRVGFERPDSHDPYRARGKLREPVACPDC
                     GAVQEAGRWRWREALPGAVKVRCPACRRVHDGLPAGEIRLSGDFLADHRDEVMARVTH
                     VADRARKRYPLQRIMQVDQDGADVRITVTSPHLARSLGLALHESFKGELELDTHAQGG
                     PRVRWSR"
     gene            complement(3115..3429)
                     /locus_tag="VF453_02745"
     CDS             complement(3115..3429)
                     /locus_tag="VF453_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686592.1"
                     /translation="MRVQTHRFDAWREAERTAHAVERALYLLQVRSPGRDSPELQALT
                     QLAGQLRARARGLFDEAMLEMKELAESLHHRRVLAEGRDVSATSAPAAPPAHACPARA
                     RP"
     gene            complement(3606..5180)
                     /locus_tag="VF453_02750"
     CDS             complement(3606..5180)
                     /locus_tag="VF453_02750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7686593.1"
                     /translation="MLLVSACVLPATIAATFGFVSAYRAVYAALVGEAQANSRAMART
                     VDGVILNAIATLDRMASSPPLSSGDIVRFEAMAREVQPYLPGTNLVLTDRDGRQLVNT
                     ALAQGTALPMHGNPGFQRTVLSTGHAAVSDLFIGGALRRPLVAIEVPVRREGAISATL
                     AMGFLPERFARIIAEQKPEPGWVVSILDSTGTIVARTHAAERFVGHKGTPALLAAMAS
                     RPQGTVATQTLEGMPVVAVYTRSEVAHWTVAVGIPEAQLANRVARWLAWLVAFSVGML
                     FVVVLLALLVAARIAGAVRSLREPARALGRGEVVEPSTMAITEAQVVADALAQASELL
                     RRRTRELGHATQAARQIQEQADAFAHAALHDPLTGLPNRARFMGALEERIETRVATGG
                     TFAVLFVDLDDFKPVNDVHGHAVGDELLCAFASRLRAGCRGADLVARLAGDEFAVLVD
                     EHAPGESRSTVTHLRSALSRPYRVRDLTLHVSASIGVATYPLDGQDAAGLLHAADTAM
                     YLVKAAGKRRGGRASG"
BASE COUNT          784 a         2187 c         2156 g          730 t
ORIGIN      
        1 cctggcgagc cgcgcggccg cccgacgtcg agtgcgcgtg cgttgcgggc gcgcaatcgg
       61 cggccggcat tccgggcgag gcttgcgctt gccgtacgag acggcgactc gaaaggagcg
      121 accatgtccg aagccgcgcc cgtggacctg cccgaagacg tcgtcgcgct ggtgcccatg
      181 cgcgatgtcg tgctgttccc gcacgtcatg atgccggtcg gcgtcggccg ggcgaagtcc
      241 atcgccgcgc tgctgcaggc cgtgcagtcg gcccggcccc tgggcatcgt gctgcagaag
      301 gatccccacg ccgacgagcc cgggcgcgcc gagctctgcg acgtcggcac cctcgcccag
      361 gtcgtgaagc acgtgcccgg cgaaggcgtg cagcacgcga tcgtgcacgg cctgtcgcgc
      421 ttgcggatcg tgtcgctcgt cgaggggtac cccttcctcg cggcccgcat cgagcgcctg
      481 ccggaggacg ccacgccgcc cgacacgcag acggaggccc tcgccctgca gctgcgcgag
      541 cgggccgcgg agatcctcgg gctgctgccg ggcgtgccgg cggaggcggc gcacatgatg
      601 caggccgcgc gcgcgcccca ggagcttgcg gacatcgtcg cgagcctcgt cgacgccgat
      661 cccgcggaga agcaggccct gctcgagtcg gccgtcctcg ccgaccggct gcgcagcctg
      721 ctctcgctgg tcgcccggcg catccaggtg ctgcgcctgt cgcaggagat cggcgagcgc
      781 acccgccagc agctggacga tcgccagcgc cgcttcctgc tgaaggagca gctgcgcacc
      841 atccagcagg agctcggcga ggacggcgag accagcgccg agctcgagcg cctgcagaag
      901 ctggtcgccg acgcgggcat gccgccggag atcgacgagc gcgcgcggcg cgagctcgca
      961 cggctgcagc gcctgcagga ctcccccggc gaggccgccg tgctgcggac gtggctcgag
     1021 tggatgaccg agctgccctg gcgcacgccg gcgccgctgg agatcgacat cgagcgcgcg
     1081 cggctggagc tgtcgcggca gcacttcggg ctcgagccgg tcaagcgccg gatcctcgag
     1141 ttccttgccg tgcgcaagct caatccctcg gccccggcgc cgatcctgtg cttcgtcggg
     1201 ccgcccggcg tcggcaagac ctcgctcggc cagtgcatcg cgcggacgct gcagcggccc
     1261 ttcgtgcgcg tgtcgctggg cggcgtgcac gacgaggccg aggtgcgcgg gcatcgccgc
     1321 acctacgtcg gcgcgctgcc gggcaacatc gtgcaggcgc tgcgcaaggc cgggacgctc
     1381 gacccggtgc tgatgctcga cgagatggac aagctgtcgg ccagcgcgca cggcgatccg
     1441 tcggcggcgc tgctggaggt gctggatccg gagcagaacg ccagcttccg ggacaactac
     1501 ctcggcgtgc cggtcgacct gagccgcgtg accttcctgt gcaccgccaa cgtgctggag
     1561 cagatgcccg cgccggtgcg cgaccgcgtg gaggtgatcg agctgcccgg ctattcgctc
     1621 gaggaaaagg agcgcatcgc ccgcgcgcac ctgctgccgc gccaactcgc ggcgcatggc
     1681 ctgcaggcta ccgccttcga gctcgacgac gccgcgctgc gccggctggc ctccgactac
     1741 acgcgcgagg cgggcgtgcg ccagctcgac cgctgcatcg gcagcgtggc gcggcacgcg
     1801 gcgatggcgg tcgtcgagtc gcccggcacg cgtgtccacg tcaccgagcg cgacctcgcc
     1861 gccatcctcg ggccggcgcc gttcgagcac gaggcggcca tgcgccgcag cgtcgccggc
     1921 atcgccaccg gcctggcctg gacgccggcc ggcggcgaca tcctgttcgt cgaggccgcg
     1981 cgcatgcccg gcgacggcca gctgatcctg acggggcagc tcggcgacgt gatgaaggag
     2041 agcgcccgcg cggccctgac cctcgtgcgc tcgcgcgcgg ccggcctgga cgtggcgccg
     2101 gatgcgttcg cgaaggccga catccacgtc cacgtgccgg ccggcgccgt gcccaaggac
     2161 gggccgagcg ccggcgtggc catgttcgcg gcgctcgcgt cggtcttcac cggccggccg
     2221 gtgcgggcgc aggtcgcgat gaccggggag atcagcctgc gcgggctcgt cctgccgatc
     2281 ggcggcgtgc gcgagaaggt gctcgccgcc cagcgcgcgg gcgtggcgac ggtgctgctg
     2341 ccgcgccgca acgagaagga cctcgcggac atgccgccgg acacgcgcgc gggcctgcgg
     2401 gtcgtgctgg tcgacagcgt cgacgacgtc ctgcgcgagg ggctggaatg accgcgacgc
     2461 cgccgcgcga gcgggcgaga gcgctgcgac gtggacgtgc cgtcgcgcgg ccgccggtcg
     2521 gtcgtcctct acttcgcgcc gccccgtccg cccagcgcgc cagcgcccgc gtcgtgacgg
     2581 gggcctgacg gtgtccgcgg tccgtgcgtc cgctcaaggc ggacccacga gaaccgcgcg
     2641 aggctgcctg cactgcatca caccaggaga cctccgtgcc cgagcccaca tcccgttccg
     2701 cccgtcccgc ccccgctgcg caccgcgtcg ggttcgagcg tcccgacagc cacgatccct
     2761 accgcgcccg cggcaagttg cgcgaacccg tcgcctgccc cgactgcggc gccgtgcagg
     2821 aggccggccg atggcgctgg cgcgaagccc tgccgggcgc cgtgaaggtg cgctgcccgg
     2881 cctgccggcg cgtgcacgac ggcctgcccg ccggcgagat ccggctgtcg ggcgacttcc
     2941 tggcggacca ccgcgacgag gtgatggcgc gcgtgacgca cgtcgccgac cgcgcacgca
     3001 agcgctaccc gctgcaacgc atcatgcagg tcgaccagga cggcgcggac gtccgcatca
     3061 ccgtgaccag cccgcacctc gcacgctcgc tcgggctcgc gctgcacgag tcgttcaagg
     3121 gcgagctcga gctggacacg catgcgcagg gggggccgcg ggtgcgctgg tcgcgctgac
     3181 gtcgcgtccc tcggcgagca cgcgccggtg gtggagcgac tccgccagct ccttcatctc
     3241 cagcatcgcc tcgtcgaaca acccgcgagc ccttgcgcgc agttgcccgg ccagctgcgt
     3301 cagcgcctgc aattcaggcg aatcgcggcc gggcgatcgc acctgcagga ggtacagcgc
     3361 gcgctcgacc gcgtgcgccg tgcgctccgc ctcgcgccag gcgtcgaagc ggtgtgtctg
     3421 gacgcgcatg gcgcactcct cggggggggg tgcgcatacg ggacggcctg cgttctgagc
     3481 tgctggcgag aggcacgaag aggcgccagc gggaggccaa gaatcgccac tgtacgccga
     3541 cgaggccacc ctgcgcatga aatgttcgcc acgggtcgac ggcgcggcgc cgctcgagcc
     3601 gtccctcatc cggacgcacg gcccccgcgc cgcttgcccg cggccttcac caggtacatc
     3661 gcggtgtcgg cggcgtgcag caggccggcc gcgtcctggc cgtcgagggg atacgtcgcc
     3721 acgccgatgc tcgccgacac gtgcagcgtc aggtcgcgca cgcggtaggg acgcgacagc
     3781 gcgctgcgca ggtgcgtcac cgtcgatcgc gattcgcccg gggcgtgctc gtcgacaagc
     3841 acggcgaatt cgtccccggc cagccgggcg acgaggtcgg cgccccggca gccggcccgc
     3901 aggcgtgacg cgaaggcgca cagcagctcg tcgccgacgg catggccgtg cacatcgttg
     3961 acgggcttga agtcgtcgag gtcgacgaac aggaccgcga aggtgccgcc cgtcgcgacg
     4021 cgcgtctcga tgcgctcctc gagcgcgccc atgaaccggg cgcggttggg cagcccggtc
     4081 agcggatcgt gcagggccgc atgggcgaag gcgtcggcct gctcctggat ctggcgcgcg
     4141 gcctgggtcg catgcccgag ctcgcgggtg cgccggcgca ggagctccga ggcctgggcc
     4201 agggcatcgg cgacgacctg cgcctcggtg atcgccatcg tggacggctc gacgacctcg
     4261 ccccgcccga gcgcgcgggc cggctcccgc agcgagcgga ccgccccggc gatgcgcgcg
     4321 gcgaccagga gcgcgagcag cacgaccacg aacagcatgc ccacgctgaa cgccaccagc
     4381 caggcaagcc accgcgcgac ccggttggcg agctgcgcct cggggatgcc caccgccacc
     4441 gtccagtgcg cgacctcgga gcgcgtgtag accgcaacga ccggcatccc ctcgagcgtc
     4501 tgcgtcgcga cggtcccttg cggccggctc gccatcgcgg ccagcagggc cggcgtcccc
     4561 ttgtggccga cgaagcgctc cgccgcgtgc gtccgcgcga cgatggttcc cgtgctgtcg
     4621 aggatggaca cgacccagcc cggctccggc ttctgttccg cgatgatccg ggcgaagcgc
     4681 tccggcagga agcccatcgc cagcgtggcg gagatcgcgc cctcgcgccg gacggggacc
     4741 tcgatggcga ccagcggtcg ccgcagggcg ccgccgatga acaggtcgga cacggcggcg
     4801 tggccggtgg acagcacggt gcgctggaag cccggattgc cgtgcatcgg cagtgcggtg
     4861 ccctgggcga gggccgtgtt cacgagctgg cggccgtccc ggtccgtcag tacgagattc
     4921 gtgcccggca gatacggctg gacctcgcgg gccatcgcct cgaaccgaac gatgtcgcca
     4981 gaggacaggg gcggcgagga cgccatccga tcgagggtcg cgatggcgtt caggatgacg
     5041 ccatccaccg tcctggccat cgcccgggag ttcgcctgcg cttcccccac cagcgcggcg
     5101 tacacggcgc ggtaggcgga gacgaacccg aacgtcgccg cgatggtggc cggcaacacg
     5161 caggccgaga cgagcagcac gaggtaggtg cggacgctgg ccgcgcgcgg gcgcgtccgt
     5221 ccccccgggc ggtgccgacc tgcccgaagc ctgccgacga ggcgcccgcc gaatgacacg
     5281 cgttgacctt ccgagcgatg tttccgctcg actatgcccc cgagccggcc ctgcgggact
     5341 gacaaagcgc aagcgggcgc gggcgcggtg atcgccgtca accggcgccg gacacgggcc
     5401 tgcgataacg ggtcctcgcc ggcggcgacc gggtaccggc gtcgcgggat ctcgaggagc
     5461 atcgatgatg accggctcat cgacagcctg ggccctcacg ccgagcgggg ccgccgcgac
     5521 ggtcgggcca cggtccggca gcccgatccg ccgggccctg ccgccgccgc gccgatcggg
     5581 cggcctgccg ctgatggagg cgttggcacg gccctgcgcg ggcctggact tcgcccggcg
     5641 acccatcgcc gcgcagctgc tttccgacct cctctgggcc gcgtacggcg tcaaccggtc
     5701 cagcggcgac cgcaccgcgc cgtactggca ccacgccatg gtcatcgacc tgtacgcgat
     5761 caaggcgaac ggagcctggc gccacgacgc gcccgcgcac gcgctggagc gcacctcggc
     5821 ccgggacctc cgtgcgacgg cggccgcgcc ggaggcc
//
LOCUS       DASVLN010000127         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_113683, whole genome shotgun sequence.
ACCESSION   DASVLN010000127 DASVLN010000000
VERSION     DASVLN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113683"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VF453_02755"
     CDS             complement(<1..966)
                     /locus_tag="VF453_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEX7686594.1"
                     /translation="MFKNLKLATKLSGAFLIVLALMAGLGTFAVTRLTIVNGAAVDLA
                     TSWMPSVRTALSMKASLNRVRVLEFKAIASGNADVVAASEKETGDKLAEMQRNQADYE
                     KLVTSPEERAAFDSFKTEYAAYVAEHAKIVALIQQNKDDQAKVVLLGDSLRHYDALRT
                     LADKLSDINIAGGDHAAQEAQATYASARVAIIGTIAGCSVLAMLMALVITRGITVPVR
                     AAVDAANRLAAGDLTVRVPAQGTDEIGQLMSAMDGMVSKLAQVVTEVNSSAEALAGAS
                     EEVSATAQSLSQAASEQAAGVEETSASMEQMTASISQNTENAKVTD"
     gene            complement(1019..>2837)
                     /locus_tag="VF453_02760"
     CDS             complement(1019..>2837)
                     /locus_tag="VF453_02760"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEX7686595.1"
                     /translation="RGACEPAAPAGAAGGADHWHLSLRFGPDVLRNGMDPLSFIRYLG
                     TIGRVEHLETLADAVPALDALDPEGCCLGFEIALLTAADKAAIENVFEFVIDDCTLRI
                     VAPHSRVAEYVELIRGLPEGPDRLGEILVRCGSVTAHELEAALQAQAVEPAHRIGAIL
                     AEQGKVAPQVIDAALARQSQSRGRDAAARSPESQSVRVDADKLDRLINLVGELIIATA
                     GANLAAKRTRNPELIECNSTLSGLVEEVRDSALQLRMVKIGATFNRFKRVVHDVAREL
                     GKDIELRVSGEDTELDKTVVEKIGDPLMHLVRNAMDHGIDAPDVRAARGKPAQGTVSL
                     NAFHDSGSIVIQVSDDGGGLPRDKILAKGIERGLVDPARTLTDAEVYALIFEPGFSTA
                     DKITNLSGRGVGMDVVKRNITALRGSVAIDSTPGEGTTVTVRLPLTLAIINGFQVAVG
                     KSVFVVPMDVVDECIEFGAEPGHDYTDLRGQVLPFIRLRDLFEIDHAPPTAESRGHPP
                     RGRGLASERPGAAAAGTPSARQNIVVVKHAGQKFGLVVDALLGEAQTVIKPLSRMFAQ
                     VRGISGSSILGSGDVALILDVPLLMQEAQAARGPAAALS"
BASE COUNT          370 a          968 c         1045 g          454 t
ORIGIN      
        1 gtcggtgacc ttggcgttct cggtgttctg ggagatcgag gccgtcatct gctccatcga
       61 ggcgctggtc tcctcgacgc cggcggcctg ctcgctggcg gcctgcgaca gcgactgggc
      121 cgtcgcgctc acctcttcgg acgcacccgc cagcgcctcg gcgctcgagt tcacctcggt
      181 gacgacctgc gcgagcttgc tgaccatgcc gtccatcgcg gacatcagct ggccgatctc
      241 gtccgtgccc tgcgccggca cgcgcacggt caggtcgccg gcggccaggc ggttggcggc
      301 gtcgaccgcg gcgcgcaccg gcacggtgat gccgcgcgtg atgaccaggg ccatcagcat
      361 ggcgagcacc gagcagcccg cgatggtgcc gatgatggcc acgcgcgcgc tggcgtaggt
      421 cgcctgcgcc tcctgggccg cgtggtcgcc gccggcgatg ttgatgtccg acagcttgtc
      481 cgcgagcgtg cgcagggcgt cgtagtggcg caggctgtcg cccaggagga cgaccttggc
      541 ctggtcgtcc ttgttctgct ggatgagcgc cacgatcttg gcgtgctcgg cgacgtaggc
      601 cgcatactcc gtcttgaagc tgtcgaacgc cgcgcgctcc tcgggcgagg tcaccagctt
      661 ctcgtagtcg gcctggttgc gctgcatctc ggcgagcttg tcgcccgtct ccttctcgga
      721 ggcggcgacc acgtccgcat tgccggacgc gatggccttg aactcgagca cgcgcacccg
      781 gttgaggctg gccttcatcg acagggcggt gcgcacgctg ggcatccagc tggtggcgag
      841 gtcgaccgcc gcaccgttga cgatcgtcag ccgcgtgacg gcgaaggtgc cgaggcccgc
      901 catcaaggcg aggacgatga gaaacgcgcc ggacagcttg gtggcgagct tcaggttctt
      961 gaacatgatg ggttcccggt ggtggattcg gattcgtgcg aagggcgagc cctgggcgtc
     1021 aggacagggc ggccgccggg ccgcgtgcgg cctgcgcctc ctgcatcagc agcgggacgt
     1081 cgaggatgag ggcgacgtcc ccgctgccga ggatggaaga gccgctgatg ccgcgcacct
     1141 gagcgaacat cctggacagc ggcttgatga cggtctgggc ctcgccgagg agcgcgtcga
     1201 cgacgaggcc gaacttctgg ccggcgtgct tgacgacgac gatgttctgg cgcgcggacg
     1261 gcgtccccgc ggccgccgcg cccggccgct ccgaagcaag gccgcgcccc ctcggggggt
     1321 ggccgcggga ctccgcggtc gggggcgcat ggtcgatttc gaacaggtcg cgcaggcgga
     1381 tgaacggcag cacctggccg cgcaggtcgg tgtagtcgtg gccgggctcg gcgccgaact
     1441 cgatgcactc gtcgacgacg tccatcggca cgacgaagac cgacttgccc acggcgacct
     1501 ggaagccgtt gatgatcgcc agcgtcagcg gcaggcgcac cgtgacggtg gtgccctcgc
     1561 ccggcgtgga gtcgatggcg acgctgccgc gcagcgccgt gatgttgcgc ttgacgacgt
     1621 ccatgccgac gccgcgcccc gagaggttgg tgatcttgtc ggcggtcgag aagcccggct
     1681 cgaagatgag cgcgtagacc tcggcatccg tcagcgtgcg cgccgggtcg accaggccgc
     1741 gctcgatgcc cttggcgagg atcttgtcgc gcggcaggcc gccgccgtcg tcgctcacct
     1801 ggatgacgat gctgccggag tcgtggaacg cgttgagcga gaccgtgccc tgcgccggct
     1861 tgccgcgcgc ggcgcgcacg tcgggcgcgt cgatgccgtg gtccatcgcg ttgcgcacca
     1921 ggtgcatcag cgggtcgccg atcttctcga ccacggtctt gtccagctcg gtgtcctcgc
     1981 cgctgaccct cagctcgatg tccttgccca gctcgcgcgc gacgtcgtgg acgacgcgct
     2041 tgaagcggtt gaacgtggcg ccgatcttga ccatgcgcag ctgcagcgcg ctgtcgcgca
     2101 cctcctcgac gaggcccgac agcgtggagt tgcactcgat cagctcgggg ttgcgcgtgc
     2161 gcttggccgc gaggttggcg ccggcggtgg cgatgatcag ctcgccgacc aggttgatca
     2221 ggcgatcgag cttgtcggcg tcgacgcgca ccgactgcga ctccggcgac ctggccgccg
     2281 cgtcgcggcc gcggctctgg ctctgtcgcg cgagcgcggc gtcgatgacc tgcggcgcga
     2341 ccttgccctg ctcggcgagg atggcgccga tgcgatgcgc cggctcgacc gcctgcgcct
     2401 gcagcgcggc ctcgagctcg tgcgcggtga cgctgccgca gcgcaccagg atctcgccga
     2461 ggcggtcggg cccctcgggc aggccccgga tcagctcgac gtactcggcc acgcggctgt
     2521 gcggcgcgac gatgcgcagc gtgcagtcgt cgatcacgaa ctcgaacacg ttctcgatgg
     2581 cggccttgtc ggcggccgtc agcagcgcga tctcgaagcc gaggcagcag ccttcgggat
     2641 cgagcgcgtc cagcgccggc acggcgtcgg cgagcgtctc gaggtgctcg acccggccga
     2701 tggtgccgag gtagcggatg aacgacagcg gatccatgcc gttgcgcagc acgtccgggc
     2761 cgaagcgcag cgacaggtgc cagtggtcgg cgccgcccgc ggctccggcc ggcgccgcgg
     2821 gttcgcaggc gccgcgc
//
LOCUS       DASVLN010000128        18131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_436369, whole genome shotgun sequence.
ACCESSION   DASVLN010000128 DASVLN010000000
VERSION     DASVLN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 18131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18131
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436369"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VF453_02765"
     CDS             complement(<1..664)
                     /locus_tag="VF453_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7686596.1"
                     /translation="MAGCLAGCLEGLRVLDLTRLLPGPFATRHLADWGAEVLKIDEPG
                     VGDAARHLLAGREDRAAGRDSAFYRELNRGKRELRLDLRSAAGREQLLALVEGADALV
                     EGFRPGAMARLGLDWDVLRARNPRLVMASITGYGQRGPYASRAGHDINYIALAGVLSQ
                     VGTAAGELALPNFQIADLLGGTLAATSALLAALLGAQRTGRGRHVDVSMTREVWRHAV
                     VTR"
     gene            complement(759..1658)
                     /locus_tag="VF453_02770"
     CDS             complement(759..1658)
                     /locus_tag="VF453_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX7686597.1"
                     /translation="MSSQSITQTAAGDKRQQYISHELRQWIISQAQAGVAPDQVLEAM
                     KTSGWHEDVAIQALEETLKAHLAEQERAKLPKHEALPVPVPEPSVADSPSKLRVLDRD
                     VTVLMSMKLPRVVVFSGFLTDAECDQMIALAGPRMARSETVDNKTGGSEVNVARTSRG
                     MFFERGETSLIERIEKRIAALLHWPVENGEGLQVLHYKPGAEYKPHYDYFDPVHPGSA
                     TILTRGGQRVGTLLMYLNTPKKGGGTTFPDVGLEVAPIKGNAVYFSYDRAHVDTKSLH
                     GGAPVIEGEKWVATKWLREREFI"
     gene            complement(1842..2660)
                     /gene="yaaA"
                     /locus_tag="VF453_02775"
     CDS             complement(1842..2660)
                     /gene="yaaA"
                     /locus_tag="VF453_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEX7686598.1"
                     /translation="MLLLLSPAKTLDYESPLPEAVVAARAALRPAATAPQYPKDSAEL
                     IAALRGKSVAEVAALMHLSDELARLNVDRYRAWSSRATARNSRPAMWAFDGDVYDGLQ
                     ARTLDAGGIAWAQKHVLMLSGLYGVLRPLDRLQPYRLEMGSALETGRGGSLYAFWGDK
                     VAAHLNALQARDPAPVVVNLASQEYFKVTQARRGREKVLRARVVECVFEDWKAGEWKV
                     ISFFAKRARGRMARFAIDARATLPEQLQAFDADGYAFAPRASEPDRYVFRRRGA"
     gene            complement(2864..4414)
                     /locus_tag="VF453_02780"
     CDS             complement(2864..4414)
                     /locus_tag="VF453_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7686599.1"
                     /translation="MLSGASLPAWAGLQEAYEAGRHGDYATALQEFRPLADKGDRDAQ
                     FALGDMYLRGYGVPVDDVAAARWFRKAADQGDPRAQTDLGLLYEQGKGVPRDFDEAAK
                     WYRKAADQGEPNAQSNLAYLSLIGRGVPQDDVQAAHWATLAAEQGLAVAQYRLGVFYT
                     NGQGVSRDDATALHWYQAAAAQGDARAQSALGWVYSEGRGVKRDPAESLRWLRLAASQ
                     GDPTAEHNLGMALLNGHGGPRDPVEAERWIRRAADAGYAPAQVSLGLMYAAGEAVIAD
                     EKQSVAWYQKAAAQGNASGQNNLGWMLANGLGVPRDDAAALRQFRLAARKGNAFAMDN
                     LGWMAEEGRGMPADTAEALRWYRLAEARGNAPARNHLALLEARGKGMARDDAAALGGF
                     RAAAAQGLAPARHNLGYMIANGRGGLARPDKAVGMALQDMAREITPPSAEDLPAIDPA
                     TLTPAERVRSGKLAALFKNSPDFLAAVDAALAQSEFSVSATGPSSGSPHGNGTDAGTP
                     APPSATQR"
     gene            complement(4765..8373)
                     /locus_tag="VF453_02785"
     CDS             complement(4765..8373)
                     /locus_tag="VF453_02785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX7686600.1"
                     /translation="MLPDYAELLCRSNFSFLTGASSPEELVDRAAELRYSALAITDEC
                     SLSGVVHAHERATEVGLHLIVGAEMRLEAGVGGLPMRLVLLATTHRGYSNLSQWISLA
                     RRRAEKGRYEARAGDVEGHAPIEGLTGLPDCLALLLPTTGPVRIGAQIRPSAPGGADE
                     GRAVSGNVLSPAATSSQPMPSSQSVIAIARARAAATIATNKPGAGRPGGRVARQMRDG
                     AAAVDPSEPVTDTPEGVAPAALREAKPAPMPEFVNRPSAALPVESLFSQAQWLKTWFG
                     LDRGWLALPMLGQGDDDELRERVKAVARATGLRIVAVGDVLMATHSAKPLQDVLTATR
                     LQCTLGEAGHALSANAEAHLRGRARLRMLFDEAWMAETMAIAGRCKFSLSEIKYTYPQ
                     EIVPKGHTPASWLRQLTEEGAVRRFGANVPHKARRLIEHELDLIGRLKYEAYFLTVAD
                     IVRWARSRRILCQGRGSAANSAVCYCLGVTEVDPDQTEVLFERFISEERGEPPDIDID
                     FEHQRREEVIQYIYDKYGRHRAALTGVITRYRTKSAVRDVGRALGVDLDRINAVTRSL
                     HHVGGSAAGSAVERLALSLMEHGFDPDSRLGRNWIALSMKLRGQPRHLSQHPGGFLIA
                     REDVARMVPVENAAMDGRTVIQWDKDDLDVLGLLKVDILALGMLSALKRAIVFVDEKV
                     RRMGPLRPEWLRGETAPDAWSSTLTLEAIPKGDDPAVFAMLSAADSVGVFQVESRAQM
                     SMLPRLRPEAFYDLVVEVAIVRPGPIQGGMVHPYLKRREKKEAVDYPSPEVEEVLKRT
                     LGVPIFQEQVMQLAVVAADFTPGEADQLRRAMAAWKRKGGLEPFQEKLTSRMLAKGYA
                     IEFIDNIFKQIQGFGEYGFPESHAASFASLVYASSWIKRYHPDAFLAALLNSLPMGFY
                     AAPQLVRDARAHGVAVRPVDILVSDWDSTLEPALPEENVPACAERFIPSGLCAVRLGL
                     GRVQGLDEREGRRIERARARAPFVDVEDLARRAGLDDHALQCLAAADALGGLSGGRRE
                     SLWAVAGVDTRSTPLLKQTRTDEGTGPRLESLGLADAVLADYQALGLSLKCHPLALLR
                     SSLAPFKVEQAVRLNETWRSGQLARACGLVTHRQQPGTAKGVVFVTLEDETGVVNVIV
                     WPAVAAAQRRPLLGASLLTVYGVWQREGEVRHLVAKTLVDHTPMLQGLATRSRDFH"
     gene            8725..8973
                     /locus_tag="VF453_02790"
     CDS             8725..8973
                     /locus_tag="VF453_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX7686601.1"
                     /translation="MLLLKPGCECCDADLPPESPAARICSFECTFCAECADGPLGGRC
                     PNCAGELVRRPIRPPAELAAHPASTTRVFRPRGDAEPR"
     gene            9174..9770
                     /locus_tag="VF453_02795"
     CDS             9174..9770
                     /locus_tag="VF453_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686602.1"
                     /translation="MNRRLHALPLTILAALCGCAADPAAKSAADAGAAIQQAAEAPLA
                     DLNLVQTKIPPALQAAMQAPYALPPRKTCPDLAGEVTALDAALGPDLDTPATPSDPGL
                     VARGAHAIGGAANDALKGAAESVIPFRGWVRRLDGAERHSKEVAAAIAAGSVRRAYLK
                     GVGQALGCTPPAAPASALAASAVATAAPAADVPAGTVR"
     gene            9816..10037
                     /locus_tag="VF453_02800"
     CDS             9816..10037
                     /locus_tag="VF453_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HEX7686603.1"
                     /translation="MDRPIVGFHQDEAGDWVAELACGHFQHVRHDPPWTNRPWTQTAE
                     GRAGALGRTLACRKCDQGAPPDMLLPDAR"
     gene            complement(10110..10484)
                     /locus_tag="VF453_02805"
     CDS             complement(10110..10484)
                     /locus_tag="VF453_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX7686604.1"
                     /translation="MSIAPPSLTDASPPGSRLVEIRAYKLVPGTRAEFHLAMSRALEL
                     VRDYGMDVVAHGHSSEEDGYFLIRAFDDRAHLKAQQDGFYGSRIWREGPRESIVSRIA
                     VSLDTLLWLSPAAIDELRLAMS"
     gene            10726..11151
                     /locus_tag="VF453_02810"
     CDS             10726..11151
                     /locus_tag="VF453_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX7686605.1"
                     /translation="MTAQLDTATTFARVSELCLALPEVERQLQGRHADFRVRNKPFAY
                     FLDDHHGDGIVSVCCRSALGENVDRAGRDPARFYLPAYIGKRGWFGIRLDAALVDWDD
                     IGQAISLSWRLAAPGALVARHESQAAQALAAAMRAADRP"
     gene            11330..11533
                     /locus_tag="VF453_02815"
     CDS             11330..11533
                     /locus_tag="VF453_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686606.1"
                     /translation="MNALLALVLWCLLFVLCWPVALIALVLWPVVWLVTLPLRLVGIG
                     VGAAFAFLTALLFLPARALGHRR"
     gene            12191..13405
                     /locus_tag="VF453_02820"
     CDS             12191..13405
                     /locus_tag="VF453_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7686607.1"
                     /translation="MPDSTPSTRTPAPSRDAPTDIEGALHLALSSSSGTVVTDIIIHE
                     GQAIRAKSARGTLPLHQLFPGIAPFAVTRAHIAAYLAPLDGDHAAAAMTAGRETRIDP
                     ELARQHPLCFRIDGPGGASLRCSLFLRNTGELALVMRVSPGTITPLSYLNIPQDLLTA
                     FTESTSGFIVITGPTGAGKSETAMSVLDWHNAQHAGHILTLEDPVEKRLASRRSIVTQ
                     REVGVGAPSFADGMRSALRMSPDILYASEIGDAETAMQAIQGGESGSLMIATTHGKTV
                     TGALRKILGHLGPGADAMRQVLAGNLVAVVRQALVPARSGERYLLVADVLYNTGRVTQ
                     YIDKGDWTGLEAAMRQDHYLHTNEWFPMSTRLAELVEHGEIDVAEAMREASDIPTLRR
                     RLSGISPFTTYR"
     gene            complement(13436..14020)
                     /locus_tag="VF453_02825"
     CDS             complement(13436..14020)
                     /locus_tag="VF453_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX7686608.1"
                     /translation="MGKGRFEAFSDGVLAIIITIMVLELHVPHGDDLAALTAQWPIFV
                     SYVLSFIYVGIYWNNHHHLLHATSRVTGGMLWANLHLLFWLSLFPFATGWMGENHFAP
                     LPSAMYGGVLLMAAISWAILQAVILRAEGPQSLLRQALGRDWKGRFSPVLYLAGIVSS
                     FWIPGLAQALYLVVALVWLVPDRRIEAALRSRGD"
     gene            14607..15758
                     /locus_tag="VF453_02830"
     CDS             14607..15758
                     /locus_tag="VF453_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7686609.1"
                     /translation="MTAFGNAMRLAPRDPEPIYNLGVALEHFGDWSGSLMAFEAALEC
                     APEHADAAWNLSLALLRHGELERGFRHYESRFRRLAPAPRACARPAWSGEPLAGLRVL
                     VWAEQGFGDTLQFLRFVPELARRGAQVILEVPRELRGLASRLPGAPEVRVPGEALPRF
                     DVHLALMSLPFALGLRGNELAAAAPYLQADAARVADWRTRLHSLGWKPARALAVGVVW
                     ATNPALPNARDRSPGLATLAPLLKAPGLRWFSLQKGTGEAELAATAAAGPITDLAAHI
                     DDFDDTAAIVANLDVVVTCDTSVAHLAGALGKPTFVLLPFVPDWRYGLAPHDTPWYPS
                     MRLFRQSRRGHWGDARERVAGALARAVAACERVDGTCGEPSTTPAAALT"
     gene            16059..16760
                     /locus_tag="VF453_02835"
     CDS             16059..16760
                     /locus_tag="VF453_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEX7686610.1"
                     /translation="MKSSRKSSPALSEADAGFQPRDHTGFELPRALPSTVTPLLPAAT
                     RCSPARADGAPGRLHQRPISAMNQFDPLRKVMHVDEAVARFAALPGTVFTTGVFDLLH
                     RGQVARLAEARQLGERLVVGVYSDTSAALLDKTSDRPLHGEGDRAYQVAALESVDAVV
                     IFRESTPIPLLMRLRPSVYVRGGEQDVNRLAETALTHGWGGHVVALPFLDGFSTSSLL
                     ERIRAGSPALYGEES"
     gene            16757..17356
                     /locus_tag="VF453_02840"
     CDS             16757..17356
                     /locus_tag="VF453_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX7686611.1"
                     /translation="MNRATSAIGRAVFLARDGVLNEDRPNGVGRPEDFAWLPGAIPAL
                     RALRRGGWRLVVTTNQPAIARGLLGPAEYDNLTGWMRAELRRHGVALDGVYHCPHLPD
                     APLAAWRRQCACHKPHAGMFRRAARDLQLDLADCVVIGDSGDDVAAARAARVAAAIRI
                     ADPADTAALVASHAAADFTCNDLAQAADWLLHHEKRQRA"
     gene            complement(17491..>18131)
                     /locus_tag="VF453_02845"
     CDS             complement(17491..>18131)
                     /locus_tag="VF453_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX7686612.1"
                     /translation="ACAIDAARAVAAGGDRATAAALTRPPGHHAGPDFFGGYCFLNNA
                     ALAAQALRDAGARRVAVVDVDYHHGNGTQTLFYDRADVLTVSLHGDPTTEYPFYLGYA
                     DERGEGAGFGANLNLPLPAGTGFDRWSAALGDALQAVRRFGADALVVALGVDTFEGDP
                     ISRFALRSEDYLRVGERLAAEGLPTVFVMEGGYAVAEVGVNVVNVLQGFAGG"
BASE COUNT         2607 a         6730 c         6224 g         2570 t
ORIGIN      
        1 cgcgcgtgac gaccgcgtgg cgccagacct cccgcgtcat cgagacgtcg acatgccggc
       61 cgcgccccgt gcgctgcgcg ccgagcagcg cggcgagcag cgcgctcgtc gccgccagcg
      121 tgccgcccag caggtcggcg atctggaagt tgggcagcgc gagctcgcct gccgccgtcc
      181 cgacctggct cagcacgccg gcaagtgcga tgtagttgat gtcgtggcct gcccgcgacg
      241 catagggccc gcgctgtccg tagccggtga tcgaggccat caccaggcgc ggattgcgcg
      301 cccgcagcac gtcccaatcc aggcccaggc gtgccatcgc gcccggccgg aaaccttcga
      361 cgagcgcgtc cgcgccctcc accagcgcca gcagctgctc gcgtccggcc gcgctgcgca
      421 ggtccagccg cagttcacgc ttgccgcgat tgagctcgcg gtagaacgcg ctgtcgcggc
      481 cggccgcgcg atcctcgcgg ccggccagca ggtggcgcgc cgcgtcgccg acgcccggct
      541 cgtcgatctt gaggacctcg gcgccccagt cggccaggtg ccgcgtggcg aacgggcccg
      601 gcagcaggcg ggtgaggtcg aggacgcgca ggccctccag gcagcccgcc aggcagcccg
      661 ccaggccggg atccgcgtcc ggggccgtcg tcgcggcgtc ggtgccggag ggggaaccat
      721 cggctgcaac cgaggcggcc gcggcccgcg cgaccggctc agatgaactc acgctcgcgc
      781 agccacttgg tcgcgaccca cttctcgccc tcgatcaccg gagcgccgcc atgcaggctc
      841 ttggtgtcga catgggcccg gtcgtagctg aagtagaccg cgttgccctt gatcggcgcg
      901 acctccaggc cgacatcggg gaaggtcgtg ccgccgccct tcttgggcgt gttcaggtac
      961 ataagcaacg tgccgacgcg ctggcctcca cgggtcagga tcgtggccga gccggggtgc
     1021 accgggtcga agtagtcgta gtgcggcttg tactcggcgc ccggcttgta gtgcaggacc
     1081 tgcaggccct cgccgttctc gaccggccag tgcagcagcg ccgcgatgcg cttctcgatg
     1141 cgctcgatca ggctcgtctc gccgcgctcg aagaacatgc cgcggctggt gcgggcgacg
     1201 ttgacctcgc tgccgccggt cttgttgtcc acggtctccg agcgcgccat gcgcgggccc
     1261 gccagcgcga tcatctggtc gcactcggcg tcggtcagga agccgctgaa gaccaccacg
     1321 cgcggcaact tcatgctcat gagaaccgtc acgtcgcgat ccagcacccg cagcttgctg
     1381 ggcgaatccg cgacggacgg ctcgggcacc ggcaccggca gcgcctcgtg cttgggcagc
     1441 ttggcgcgct cctgttcggc gaggtgggcc ttgagcgtct cctcgagcgc ctggatcgcg
     1501 acgtcctcgt gccagccgct ggtcttcatc gcctccagca cctggtctgg ggccacgccg
     1561 gcctgcgcct gggagatgat ccactggcgg agttcgtgcg agatgtactg ttgccgcttg
     1621 tcgccggcgg cggtctgcgt gatggattgc gaggacatgg gactcaagct ccgggacgag
     1681 gcatcgacga tctcggcccg acccggaccg cgatctccaa tccccgtatt ctggcagcgc
     1741 gcaacggtcg agctcgcagg gaatccctcc attccgcggc ctgcgggcgc caccggggcc
     1801 gaattcacag gattcgcgca gcggaccggg tcgatgcccg ctcaggcgcc gcgccgccgg
     1861 aacacgtagc ggtcgggctc cgacgcacgc ggcgcgaacg cgtacccgtc ggcatcgaag
     1921 gcctggagct gctccggcag cgtcgcccgc gcgtcgatcg cgaagcgtgc catccggccg
     1981 cgggcgcgct tggcgaagaa gctgatcacc ttccactcgc cggccttcca gtcctcgaag
     2041 acgcattcga ccacacgggc gcgcaacacc ttctcgcggc cgcgacgcgc ctgcgtgacc
     2101 ttgaaatact cctgcgacgc gagattcacc accaccggcg ccgggtcgcg cgcctgcagc
     2161 gcattgaggt gcgcagccac cttgtcgccc cagaacgcgt agagcgagcc gccgcgcccg
     2221 gtctccagcg ccgagcccat ctcaagccgg tacggctgca gccgatccag cggacgcagc
     2281 acgccgtaga ggccgctgag catcagcacg tgcttctgcg cccaggcgat cccgccggcg
     2341 tccagcgtgc gcgcctgcag gccgtcgtag acgtcgccgt cgaaggccca catcgcgggg
     2401 cggctgttgc gcgcggtcgc gcgcgacgac catgcgcgat agcgatcgac gttcaggcgc
     2461 gcgagctcgt cggagaggtg catcagcgcg gcgacctcgg ccacggactt gccgcgcaac
     2521 gcggcgatca gctcggcgga gtccttcggg tattgcggcg ccgtcgccgc cggccgcagg
     2581 gccgcccggg cggcgacgac ggcctcgggc agcggggatt cgtaatcgag cgtcttcgcg
     2641 ggcgacagga gcagcagcat ggcgggcagt gtagcgacgc ctcgacgggg agtgcgggcc
     2701 ggccccgcgg gcgcggaagg cactgcggca cgcaaaccgc ttcgcccagg cgcaccggac
     2761 gagaaggcca ccggcgacac gacgcccccg agagcagcgt catgcgcagg gccgcgacgc
     2821 cgccctccga atcgcgtcgg gcaggccggc ggtcccggag gtctcagcgc tgcgtcgccg
     2881 aggggggtgc cggcgtgccc gcgtccgtgc cgttcccgtg cggcgagccg gaagacgggc
     2941 ccgtcgccga cacggaaaac tccgactgcg ccagcgcggc gtcgaccgcc gcgaggaagt
     3001 ccgggctgtt cttgaacagg gcggccagct tgccgctgcg cacgcgttcg gcaggcgtca
     3061 aggtggccgg atcgatcgcg ggcagatcct ccgcgctggg cggcgtgatc tcgcgggcca
     3121 tgtcctgcag cgccatgccc accgccttgt ccggccgcgc gagtccaccg cgcccgttgg
     3181 cgatcatgta gccgaggttg tgccgcgccg gcgcgaggcc ttgcgccgcc gccgcccgga
     3241 atccccccag cgccgccgca tcgtcccgcg ccatgccctt gccgcgagcc tccagcagcg
     3301 cgagatggtt gcgggcaggc gcgttgccgc gcgcctcggc caggcgatac cacctcagcg
     3361 cctccgcggt gtccgccggc atgccccggc cttcctccgc catccagccg aggttgtcca
     3421 tcgcgaacgc gttgcccttg cgcgccgcga ggcggaactg ccgcagcgcc gccgcatcgt
     3481 cgcgcggcac gcccagcccg ttggccagca tccagccgag gttgttctgg cccgaagcgt
     3541 tgccctgcgc cgcagccttc tggtaccagg cgacggactg cttctcgtcc gcgatgacgg
     3601 cctcgcccgc ggcgtacatc aaccccaggc tgacctgcgc cggcgcgtag cctgcgtcgg
     3661 cggcgcgacg gatccagcgc tccgcctcga ccgggtcgcg cggcccgcca tggccgttga
     3721 gcaacgccat gccgaggttg tgttccgctg tcggatcgcc ttgtgacgcc gccagccgca
     3781 gccagcgcag cgattcggcc gggtcgcgct tgacgccccg accctcgctg tagacccagc
     3841 cgagggcgct ctgcgcacgt gcgtcgccct gggcggcggc ggcctggtac caatgcaagg
     3901 cggtcgcgtc gtcgcgcgag acgccctgcc cgttcgtgta gaagaccccg agccggtact
     3961 gcgccacggc caggccctgc tccgcggcga gcgtggccca gtgggctgcc tggacatcgt
     4021 cctgcggtac cccgcggccg atcaggctga ggtaggcgag attgctttgc gcgttgggct
     4081 cgccctggtc ggctgccttg cggtaccact tggccgcttc gtcgaagtcg cgcgggacgc
     4141 ccttgccttg ttcgtagagc aagccgaggt cggtctgcgc ccgcgggtcg ccctggtccg
     4201 cggccttgcg gaaccagcgc gcggcggcaa cgtcgtcgac cggcacacca tacccccgca
     4261 ggtacatgtc gccgagcgcg aattgcgcgt cgcggtcgcc cttgtcggcc aggggccgga
     4321 actcctgcag cgcggtcgcg tagtcgccgt ggcggcccgc ttcgtaggct tcctgcaatc
     4381 cggcccacgc tggcaggctc gcaccgctca gcatcaggac cagtccgagc gtggccgatc
     4441 cccgccgcca tatccaggag gcgtgcgacc gctccgccgt caccctcggg ctcgatggct
     4501 ggcggacgga cgaggacttc atgtcgggcg gttcctggat gacggttcgc gtcgggtccc
     4561 ggccgggctc cgtccacgcg gggcgcctgg gccgccctcc tgggcgacga ggcgacgcag
     4621 cccgaattat cgatgcgggc cccgcgtccg atgccggggg cttgccttag gtcgtcgcag
     4681 gcatgcgaca cggcgccgtc cgggggtcgt actgggcgcg gcctggccgt gcgacgggtg
     4741 gaagagcgcg cggacatgcg gtcctcaatg gaaatcacgg ctgcgcgtgg ccagcccctg
     4801 cagcatcggc gtatggtcga cgagcgtctt cgcgacgagg tgccgcacct cgccctcgcg
     4861 ctgccacacg ccgtacaccg tcagcagcga cgcaccgagc agcgggcggc gctgcgccgc
     4921 cgcgaccgcg ggccacacga tcacgttgac cacgccggtc tcgtcctcca gcgtgacgaa
     4981 gacgacgccc ttggccgtgc ccggctgctg ccggtgcgtg acgagcccgc aggctcgcgc
     5041 cagttgtccg gagcgccacg tctcgttcaa gcgcaccgcc tgctcgacct tgaacggcgc
     5101 cagcgacgag cgcagcaacg cgaggggatg gcacttcagc gacagtccca gtgcctggta
     5161 atcggccagc accgcgtcgg caagccccag ggattccagt cgaggcccgg tgccttcgtc
     5221 ggtgcgggtc tgcttaagca gcggcgtcga tcgcgtgtcc acacccgcga cggcccacag
     5281 cgactcgcgc cgtccaccgc tcagtccacc cagggcgtcg gcggcggcga ggcattgcag
     5341 ggcgtggtcg tccaggccgg cgcggcgggc gagatcctcg acatcgacga aaggcgcacg
     5401 ggcgcgcgcg cgctcgatgc gtcggccttc ccgttcgtcg agtccctgga cgcgcccgag
     5461 accgagccgc accgcgcaca ggccggacgg gatgaagcgc tcggcgcacg cgggaacgtt
     5521 ctcctccggc agcgccggct cgagcgtgga atcccagtcg ctcacgagga tgtccaccgg
     5581 ccgcaccgcg accccgtgcg cgcgcgcatc gcgcacgagc tgcggcgccg cgtagaagcc
     5641 catgggcagg ctgttgagca aggcggcgag gaacgcgtcc gggtgatagc gcttgatcca
     5701 ggaactggca tagaccagcg acgcgaagct cgccgcgtgg ctctcgggga agccatattc
     5761 gccgaagccc tggatctgct tgaagatgtt gtcgatgaat tcgatcgcgt aacccttggc
     5821 gagcatccgc gaggtgagct tttcctggaa cggctccagg ccgcccttgc gcttccacgc
     5881 cgccatcgcg cgccgcagct ggtcggcctc gccgggcgtg aaatcggcgg cgacgaccgc
     5941 cagctgcatg acctgctcct ggaagatcgg cacgcccagc gtgcgcttca ggacttcctc
     6001 gacctcgggg ctgggatagt ccaccgcctc cttcttctcg cgccgcttca ggtaggggtg
     6061 caccatgccg ccctggatcg ggccgggccg cacgatcgcg acctcgacga cgaggtcgta
     6121 gaacgcctcg ggtctcagcc gcggcagcat gctcatctgg gcccggctct ccacctggaa
     6181 gacaccgacc gaatcggccg ccgacagcat cgcgaagacg gccgggtcgt cgcctttcgg
     6241 gatcgcttcg agggtcagcg tcgaactcca ggcatccggc gcggtctcgc cccgcaacca
     6301 ttccggccgc agcggcccca tccggcggac cttctcatcg acgaacacga tggctcgctt
     6361 gagcgcgctg agcatgccca gcgccaggat gtcgaccttg agcaagccga gcacgtcgag
     6421 gtcgtccttg tcccactgga tcacggtgcg gccatccatg gcggcgttct ccacgggcac
     6481 catgcgggcg acgtcctcgc gggcgatgag gaagcctccg ggatgctggc tcaggtgccg
     6541 cggctggcca cgcagcttca tgctcagcgc gatccagttg cggccaaggc gggaatcagg
     6601 atcgaagcca tgctccatca acgagagggc cagccgctcg accgcgctgc ccgccgcgga
     6661 gccgccgaca tggtgcaggc tgcgcgtgac cgcgttgatg cggtcgagat ccacgccgag
     6721 cgcacgtccg acgtcccgta cggccgactt ggtgcgatag cgcgtgatca cgccggtcag
     6781 cgccgcccga tgacgtccgt acttgtcgta gatgtactgg atcacctcct cgcggcgctg
     6841 gtgctcgaag tcgatgtcga tgtcgggtgg ctcgccgcgc tcctcgctga tgaagcgctc
     6901 gaacaggacc tcggtctgat cgggatcgac ctcggtgacc ccgaggcagt agcacacggc
     6961 cgaattggcg gcgctgccgc gcccctggca caggatgcgt cgcgatcgcg cccagcggac
     7021 gatgtcggcc accgtcagga agtaggcctc gtacttcagc cgcccgatga ggtccagctc
     7081 gtgctcgatg agtctcctcg ccttgtgcgg cacgttcgcc ccgaagcggc gaacggcccc
     7141 ctcctcggtc agctggcgca gccacgaggc gggcgtgtga cccttgggca cgatctcctg
     7201 gggataggtg tacttgatct cgctcagcga aaacttgcaa cgtcccgcga tcgccatcgt
     7261 ctcggccatc caggcctcgt cgaagagcat gcgcaggcgg gcccgcccgc gcaggtgcgc
     7321 ttccgcgttg gccgacaagg catggcctgc ctcgcccagg gtgcactgga ggcgcgtggc
     7381 ggtgaggacg tcctgcaagg gcttggccga atgggttgcc atcaggacgt ctcccaccgc
     7441 cacgatcctg agcccggtgg cgcgggcgac ggccttgacg cgctcgcgca gttcgtcgtc
     7501 gtcaccctgc cccagcatcg gcaacgcgag ccagccgcgg tcgaggccga accaggtctt
     7561 cagccactgc gcctgggaga acagcgattc gaccggcagc gctgccgacg ggcggttcac
     7621 gaattccggc atcggcgcag gcttggcctc gcgaagcgca gcgggcgcga cgccctccgg
     7681 cgtgtccgtc accggctcgg atggatccac cgctgccgcc ccatcgcgca tctggcgagc
     7741 cacacgacca cccggacggc ccgcgcccgg cttgttggtg gcgatcgtcg cggccgcccg
     7801 tgcccgcgcg atggcgatga cgctctggct ggagggcatc ggctggctcg acgtggccgc
     7861 gggtgaaagt acgtttccgg acacggctcg cccctcgtcg gcccctcctg gcgccgatgg
     7921 ccggatctgg gcgccgatcc gcacggggcc ggtcgtgggc agcagaaggg cgaggcagtc
     7981 cgggagcccg gtcaggccct cgatgggcgc atggccctcg acatcgccgg cccgggcttc
     8041 gtagcggccc ttctcggccc gccgccgcgc caggctgatc cattgcgaca ggttgctgta
     8101 accccgatgc gtggtcgcca gcagtacgag ccgcatgggc aggccaccga cgcctgcctc
     8161 cagccgcatc tcggccccaa cgatgaggtg cagtccgacc tcggtggcac gttcatgggc
     8221 atgcaccacc ccggacagcg agcactcgtc ggtgatcgcc agggccgagt agcgcaactc
     8281 ggcggcacgg tcgaccagtt cctccgggga ggaagccccg gtgaggaagc tgaagttgct
     8341 gcggcagagc agctcggcgt agtcaggcag catggcgtag ggaatgcgat acgaggacaa
     8401 cgtacctcgc caggaacgtg gtcacgccac ggtcggccaa tgcagcggcc actccgaaga
     8461 ggcgccggat gtcgacatcg agctgctgcg tccaggcatc agggccttca cgtcgagccg
     8521 catcgtcgat actgtatttt catacagcat acgcgccagg cgcgaccctg acaagacccg
     8581 aggcattcgc cggtgcgccg cccgccggaa acggagccca acccgccgac gatgtccgga
     8641 atcgtcctgc cgacgtcccg tgccggcctc gcgccgaggc gagccggaca ctacgctagc
     8701 ggcacactct cacgaggaca tccgatgctt ctcctcaagc ccggctgcga atgctgcgat
     8761 gccgacctgc cgcccgaatc tccggccgca cgcatctgct cgttcgaatg caccttctgc
     8821 gccgagtgcg ccgatggccc cctcggcggc cgctgcccca actgcgcagg agagctggtg
     8881 cgccgcccga tccgaccgcc cgcggagctc gcggcacacc cggcctcgac gacgcgcgtc
     8941 ttcaggccca gaggcgacgc cgagccccgc tgacccgaac agggccgtgc gaagacgctc
     9001 ttcccacgcc gagcgcgcca tttcgcccac ttaacccccc gtccggcgcg gcggagttct
     9061 tgcattcggc tccaagaatg gcgtcatgca ccacgccatc tccgcgaccc cgaaccgttc
     9121 gcccaaccgt tcgcccaccc ttcgcctgct cgactgctgg tacgctcggc cggatgaacc
     9181 gacgcctgca cgccctcccg ctcacgatcc tcgccgccct ctgcggctgc gcggccgacc
     9241 ccgcggccaa gagtgcggcc gatgccggcg cggcgatcca gcaggcggcg gaggcgcccc
     9301 tggccgacct caatctcgtg cagacgaaga tccctccggc gctgcaggcc gcaatgcagg
     9361 ccccgtacgc cctgccgccg cgcaagacct gcccggatct cgccggcgag gtgacggccc
     9421 tggacgcggc gctgggcccg gacctcgaca cgccggcgac gccctccgat ccgggcctgg
     9481 tcgcgcgcgg cgcgcatgcg atcggcggcg cggccaacga cgcgctcaaa ggcgcggccg
     9541 agagcgtgat cccgttccgc ggctgggttc gtcgcctgga cggtgcggag cgccactcca
     9601 aggaggtcgc ggccgccatc gccgcgggct cggtgcgccg ggcctacctc aagggcgtgg
     9661 gccaggcact cggttgcacg ccgcccgccg ccccggcgtc cgcactcgcg gcttccgcgg
     9721 tggcgaccgc ggcaccggcg gccgacgtcc ccgccggaac cgttcgttga cgcatgccgc
     9781 cgtctcgcac gccagcatcg cacgcgacgc aagccatgga tcgccccatc gtcggattcc
     9841 accaggacga ggcgggcgac tgggtcgccg agctcgcctg cggccacttc cagcacgtgc
     9901 gtcacgatcc gccgtggacg aaccgcccct ggacgcagac cgcagaaggg cgagcagggg
     9961 cactgggtcg gacgctcgcc tgccgcaagt gcgaccaggg cgccccgccg gacatgcttc
    10021 tgcccgacgc ccgctgaggc agcgacgcgc gggaccgtgt cgcttcgcgc tgcccacgcc
    10081 agcgtgcgtg aagctggcgg gacgcccgat caggacatcg caagccgcag ttcgtcgatc
    10141 gcggcaggcg acagccacag cagggtgtcg agcgagacgg cgatgcgcga cacgatggat
    10201 tcccgcgggc cctcccgcca gatcctcgag ccgtagaagc cgtcctgctg cgccttcagg
    10261 tgcgcgcggt cgtcgaacgc gcggatgagg aaatagccgt cctcctcgct cgaatgaccg
    10321 tgggcgacga cgtccatccc gtagtcgcgc accagctcca gcgcccgaga catcgcgagg
    10381 tggaactccg cgcgcgtccc ggggacgagc ttgtaggcgc ggatctcgac cagtcgggag
    10441 cccggaggcg aagcatcggt gagcgagggc ggggcgatgg acatgtcgtg tcgcgtgaaa
    10501 agtcgggagt gaacgttcga aggccgagcc cgcagtgtgc ctgtcggcca ggtcaggatc
    10561 cgtcatgttt ttacacggcg cctcgcaacg gctagggtga aaccagcccc taaggcgaag
    10621 cggcgctgcc gcagcggcct gcgcgacggt gcgcgcgatc cggcgtgcca aggggttgac
    10681 gcggagcgtc gagcgggcat gctcccgcga ttgccggagg cctcgatgac cgcccagctc
    10741 gacactgcca ccaccttcgc ccgggtctcc gagctctgcc tcgcgctgcc ggaggtggag
    10801 cgccagctcc agggccgcca tgccgatttc cgcgtgcgca acaaaccgtt cgcctatttc
    10861 ctcgacgacc accatggcga cggcatcgtc tccgtctgct gccgctcggc gcttggcgag
    10921 aacgtcgacc gcgcggggcg tgatccggcg cgcttctacc tgcccgccta catcggcaag
    10981 cgcggctggt tcggcatccg gctggacgcc gcactggtcg attgggatga cataggccag
    11041 gcgatatcgc tcagctggcg gctggcggcg cccggtgccc tggtcgcgcg ccacgagagc
    11101 caggccgcgc aggcgctggc ggcggcgatg cgggcggcgg atcgaccctg agtccgcccg
    11161 caccgagcct cgcgacggat cggtcgtcgc cccggcaagc ctgccatcca tccgccggag
    11221 ccgcagctgg tcgcatgcgc gttgacgccg cccggtggcc gcgcgatcgt ggcgacatcg
    11281 ggacggcgcg cattcgcgag cctgccctgg aacagacgaa agtcctgcga tgaatgcgct
    11341 tctcgccctc gtgctctggt gcctgctgtt cgtgctgtgc tggccggtcg cactgatcgc
    11401 cctggtgctg tggcccgtcg tgtggctggt cacgctgccg ctgcggctcg tggggatcgg
    11461 cgtgggggcc gccttcgcgt tcctgacggc gctgctgttc ctgccggcgc gcgcgctggg
    11521 gcaccgccgc tgaggtcgat ccgccggcgc gtcgcagagc cggcaaaaag ggccggaaat
    11581 cgaccgatcc gcgcagggcg gcgcgcgcgc ggcgcgtcac aatcacctgg acggccaccc
    11641 gttcccgcgc cgacggccgt cgacaccgcg cgaatccgcg ccaccgcctc gacgccgcga
    11701 ggcgacaccg cccggcaact ctgggggcgc atcgcgagcc ttgacacgct cgcgccatcg
    11761 tccgactcgc gcgatgcgag gcggatcaat tccacgacgc cgtcgccgac ggcctgcccc
    11821 gacgagtcga cgacgtcccg caccctctcg cgcggcgtca cccttgtcgg acgcgacgca
    11881 ttcgacgcgg atcgaccgct ccccggcatg ttccgcggcg gcggtgggcg atcccgtcgc
    11941 accgactcgc gaatccgcgg cccggccccg cgccgggcct gcccccgtcg atgcggcgtc
    12001 gcgacgccgt ccatcggccg tcgatacccg cgccgcggcg atgcgcttcg tgcgccgccg
    12061 ggtatccaag cccccgagcg tccgcgcgcc tgaggcgtcg cgcgacgcgc cagctcgcca
    12121 agggcaactt gcgcctgcaa tcgaggcgcg cccgcgtcgc cccgcccgcc caccctcgtc
    12181 aggagagccc atgcccgact ccacccccag cacccgaacg cccgcaccat cgcgcgacgc
    12241 cccgaccgac atcgagggtg ccctgcacct cgcgctgtcg tcgagctcgg gcaccgtggt
    12301 caccgatatc atcatccacg aaggccaggc gatccgcgcg aagtccgcgc gcggcacctt
    12361 gcccttgcac cagctgttcc cgggcatcgc gccgttcgcg gtgacgcgcg cccacatcgc
    12421 ggcgtacctc gcgccgctcg acggcgatca cgcggccgcc gcgatgaccg cgggacgcga
    12481 aacccggatc gaccccgagc tcgcgcggca gcacccgctg tgcttccgca tcgacgggcc
    12541 aggcggcgcg agcctgcgct gcagcctgtt cttgcgcaac accggtgaac tcgccctcgt
    12601 catgcgcgtc tcgccgggca cgatcacgcc gctgagctac ctgaacatcc cgcaggacct
    12661 cctgaccgca ttcaccgagt ccacgagcgg cttcatcgtg attaccggac cgacgggcgc
    12721 tggcaagagc gagaccgcga tgtccgtcct cgactggcac aacgcccagc atgccggcca
    12781 catcctcacg ctggaggatc cggtcgagaa gcgcctcgcc tcgcgcaggt ccatcgtgac
    12841 gcagcgcgag gtcggcgtcg gcgcgccgag cttcgccgac ggcatgcgtt cggcgctgcg
    12901 catgagcccc gacatcctgt acgcctcgga gatcggcgat gccgagaccg cgatgcaggc
    12961 catccagggc ggcgaatcgg gctcgctcat gatcgccacc acccacggca agaccgtgac
    13021 gggtgcgctt cgcaagatcc tcggccacct gggcccgggg gccgatgcga tgcggcaggt
    13081 gctcgcaggc aacctcgtcg ccgtggtgcg ccaggcgctg gtgccggcgc gcagcggcga
    13141 gcgctacctc ctggtcgccg acgtcctcta caacaccgga cgcgtgacgc agtacatcga
    13201 caagggcgac tggacgggac tcgaggccgc gatgcgccag gaccactacc tgcacacgaa
    13261 cgaatggttc ccgatgagca cccggctcgc cgagttggtc gagcatggcg agatcgacgt
    13321 cgccgaggcg atgcgcgagg cttccgacat tcccacgctg cggcgccggc tctcggggat
    13381 ctcgccgttc accacctatc gctgaagccg ggcgatgccc acggcgagcc gcctctcaat
    13441 cgccgcgcga ccgcagcgcg gcctcgatgc gccggtcggg cacgagccag accagcgcca
    13501 cgaccaggta cagcgcctgc gcgaggccag ggatccagaa cgacgacacg atgccggcca
    13561 ggtagagcac gggcgagaag cgacccttcc agtcgcgccc cagcgcttgc cgcagcagcg
    13621 actgcggccc ttccgctcgc aggatgacgg cctgcaggat cgcccacgag atcgcggcca
    13681 tcagcagcac gccgccgtac atggccgagg gcagcggcgc gaaatggttc tcgcccatcc
    13741 agcccgtggc gaaggggaac agcgacagcc agaacaacag gtgcaggttc gcccacagca
    13801 tgccgccggt gacgcggctc gtcgcgtgca gcaggtggtg atggttgttc cagtagatgc
    13861 cgacgtagat gaagctcagc acgtagctca cgaagatcgg ccactgggcg gtgagcgcgg
    13921 cgaggtcgtc cccgtggggc acgtgcaact cgagcaccat gatcgtgatg atgatggcca
    13981 gcacgccatc gctgaatgct tcgaaccgcc ccttgcccat cgtccctccg cagtcggtct
    14041 gcgcgccgcg cgcccgggcg cattgtcgcc ggccgcacgt cggccgattt gccggtctcg
    14101 cagggcgcta ttccgcgcgc cgcccgccgc cgaggccact aagctcgatg gcgatggagc
    14161 ccgccgtccc gaacccgact tcagtatctc gcccggaacg cgatgcggcg cgacggcgtc
    14221 cggagaactc cggcacgaag cccggccgcg ccctgggggc ggcggtcccc gccgtcgcga
    14281 cccgcgctcc ctgcagcctt tccccgcgca ccgcggcggc cgcgagcaca ccagacgcca
    14341 acgccacggc gctgctgcag catgcgctgc acaagcgatc gcaaggcctc gatggacagg
    14401 cactggccct cacgcgagcc gcgctcgcga cggagccgtc gcgcgccgac gcgtggacgc
    14461 tgctgggcat cctcgagcag cgggccggac gcgacgcgga ggcggaggcg gcctaccggg
    14521 cggcgatcgg cgtccatccc ggttatgccg acgcctggac caacctcggc aacctgctgc
    14581 gcgaccgcgg cgaccgcaac ggcacgttga cggcgttcgg caacgcgatg cggctggctc
    14641 cgcgcgatcc ggagcccatc tacaacctcg gcgtggcact cgagcatttc ggcgactggt
    14701 ccggctcgct gatggcattc gaagcggcac tggaatgtgc tccggagcac gcggacgccg
    14761 cctggaacct gtcgctcgca ctgctgcgac atggcgagct cgagcgcggc ttccggcact
    14821 acgagtcgcg tttccggcgc ctcgcacccg ccccgagggc atgcgcccgg ccggcctgga
    14881 gcggcgagcc gctcgccggc ctgcgcgtgc tcgtctgggc ggaacagggc ttcggcgaca
    14941 cgctgcagtt cctgcgcttc gtccccgaac tggcgcgtcg cggcgcgcag gtcatcctgg
    15001 aggtaccccg cgagttgcgc ggcctcgcat cgcggcttcc cggcgctccg gaggtgcgcg
    15061 tcccgggcga ggcgctgccg cgcttcgacg tccatctggc gctgatgagc cttcccttcg
    15121 cgctcggcct ccgcggaaac gagctggcag ccgccgctcc ctacctgcag gccgatgccg
    15181 cccgggttgc cgactggcgg acacgcctgc attcgcttgg atggaagccg gctcgggcgc
    15241 tcgccgtcgg ggtggtgtgg gcgacgaacc cggcccttcc caacgcgcgc gatcgttcgc
    15301 cgggactcgc gacgctggca ccgctgctga aggcgcccgg actgcgctgg ttctcgctgc
    15361 agaagggcac gggcgaggcc gaactcgccg ccaccgcggc agccgggccc atcaccgacc
    15421 tggcggccca catcgacgac ttcgacgaca ccgccgccat cgtcgcgaac ctggatgtcg
    15481 tcgtgacctg cgacacatcg gtcgcccatc tcgcaggcgc gctgggcaag ccgaccttcg
    15541 tgctgctgcc tttcgtgcca gactggcgct acggcctggc gccgcacgac acgccttggt
    15601 acccgagcat gcgtctgttc cggcagtcga ggcgtgggca ttggggcgat gcccgggagc
    15661 gggtggccgg agcgctggcg cgagccgtgg cggcctgcga gcgcgtcgac gggacctgcg
    15721 gcgagccgtc cacgacgccg gctgcggccc tgacctgacg cgccagcggg acgagctccc
    15781 ggttccggcg gcgatgactc tgtgcgggtg gtggcgccgg aagtcgcgtc catcagcccc
    15841 ccatcgccgc ggcggcccgg ctcgatcgtc cgcgaggccg agtgtccgcc gccaccctcg
    15901 ggacgcgaag cgtcgcagcg ccctgccctt ccaccggcgg cgcggcagcc cgggcagcga
    15961 cgacccggcg aagtgtttgg cagactcgtc cgcgcgcctc cgcggggcag cgggcaggcc
    16021 aattgctaca gattgctaca gccaaaaata agtcgatcgt gaaatcgagc cgcaaatcga
    16081 gtcctgccct ctccgaggcc gatgccggct tccagccgcg ggatcacact ggtttcgagc
    16141 ttccgcgcgc attgccgtcg accgtcacgc cactcctgcc ggccgcgacg cggtgctcgc
    16201 cggcacgcgc cgacggcgcg cccgggcgtc tgcatcaacg tccgatctcc gccatgaacc
    16261 aattcgatcc gcttcgcaag gtcatgcatg tcgatgaggc cgtcgcgcgc ttcgccgcgc
    16321 tgccgggcac cgtcttcacc accggcgtct tcgacctcct gcatcgcggc caggtcgcgc
    16381 gtctggccga ggcgcgccag ctgggcgaac gcctggtcgt gggggtgtac agcgacacct
    16441 cggccgccct gctcgacaag acgtcggacc gcccgcttca cggcgagggc gaccgcgcgt
    16501 accaggtcgc ggccctcgag agcgtggacg cggtcgtcat cttccgcgag tccacgccga
    16561 ttcccctgct catgcgcctg cgcccgtcgg tctacgttcg cggcggcgaa caggacgtca
    16621 accgcctggc cgagacggcg ctgacgcacg gctggggcgg ccacgtcgtc gccctgccgt
    16681 tcctcgacgg gttctcgacg agttcgctgc tggagcgcat ccgggcggga tcgccggcgt
    16741 tgtacggaga agaatcatga accgggccac gagtgcgatc ggtcgcgccg tcttcctggc
    16801 acgcgacggc gtcctcaacg aggatcggcc caacggcgtc ggccggccgg aggacttcgc
    16861 ctggctgcca ggcgccatcc cggcgctgcg cgcgcttcgc agaggcggct ggcgcctcgt
    16921 cgtgaccacg aaccagccgg ccatcgcccg cggtctcctg ggccccgccg agtacgacaa
    16981 cctcaccggc tggatgcgag cggagctcag gcgccacggc gtcgcgctgg acggcgtgta
    17041 ccactgccct cacctgcctg acgcgccgct ggccgcctgg cgacgccagt gcgcatgcca
    17101 caagccgcac gcaggcatgt tccgacgtgc ggcgcgcgac ctgcagctgg atctcgccga
    17161 ctgcgtcgtg atcggcgact cgggcgacga cgtcgccgcg gcacgcgcag cccgcgtcgc
    17221 cgcggcgatc cgcatcgccg acccggccga caccgccgcg ctcgtcgctt cccatgccgc
    17281 cgccgacttc acctgcaacg acctcgcgca ggccgccgac tggctgctgc accacgaaaa
    17341 gcggcaacgc gcctgatcgc gcctcgacgc gaacgcccct gcagcatcga gagaacgctg
    17401 accggcgcgt gggtggaaag tcgacccacg acgcagaacg ccacgcgccg cgactgcccc
    17461 ggccgtggcc gaaggttgac gcaagctctc tcagccgccg gcgaagccct gcagcacgtt
    17521 cacgacgttg acgccgacct cggccaccgc gtagccgcct tccatgacga acacggtcgg
    17581 gagaccttct gcggcgaggc gctcgccgac gcgcaggtag tcctcgctgc gcagggcgaa
    17641 gcgcgagatc gggtcgccct cgaaggtgtc gacacccagc gccaccacga gcgcgtcggc
    17701 gccaaaccgg cggacggcct gcaaggcatc gcccagcgcc gccgaccagc ggtcgaagcc
    17761 ggtgccagcc ggcagcggca ggttgaggtt ggcgccgaag ccggcgcctt cgccgcgctc
    17821 gtccgcgtag ccgaggtaga acgggtactc ggtggtcggg tcgccgtgca gcgagaccgt
    17881 cagcacgtcc gcgcggtcgt agaagagcgt ctgcgtgccg ttgccatggt ggtagtcgac
    17941 gtcgaccacg gccacgcggc gcgcgccggc gtcgcgcaac gcctgggccg cgagcgcggc
    18001 gttgttcagg aagcagtagc cgccgaagaa gtccgggcct gcgtgatggc ccggaggccg
    18061 ggtgagcgcc gccgccgtcg cgcgatcgcc gcccgcggcc accgcgcgcg cggcgtcgat
    18121 cgcgcaggcc g
//
LOCUS       DASVLN010000129         4190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_59644, whole genome shotgun sequence.
ACCESSION   DASVLN010000129 DASVLN010000000
VERSION     DASVLN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59644"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..596)
                     /locus_tag="VF453_02850"
     CDS             complement(96..596)
                     /locus_tag="VF453_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7686613.1"
                     /translation="MTQHPAVPGFYAPETFRREESVGSVLKGVLASIQSQVDRRLVDH
                     DLTHAQWVPLYKLAHGECATVAELARKLQSDPGAMTRALDRLEAKGLVARVRSSEDRR
                     VVNLELTETGREVAAIVPAVLADVLNQHLAGFTESEWRSLLGMLHRMRANGDALRAAQ
                     ASATPD"
     gene            725..2575
                     /locus_tag="VF453_02855"
     CDS             725..2575
                     /locus_tag="VF453_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX7686614.1"
                     /translation="MTANTDRAKGTTPRSLTGLMPYLRPYRGRIAAAIVFLIAAAVTT
                     LAFPVALRSLIDNGLGVSATPGASAAALNGARVMALRDHFLALFGVGAALGVFSALRF
                     YSVTWLGERVTADLRTAVYSHVVRQSPAFFETTATGEVLSRLTTDTTLVQQVVGSSLS
                     MGLRNGVMGLGALVMLVVTNPTVMSQVLGILVLVVAPSMYFGRRVRKLSRASQDRVAD
                     SSAIAAEVLNAIPVVQGYTQETREAARFSTSTEHAFETARKRTLVRAVLVAFIISATF
                     AALLWGLYQGTTAVMHGEMTAGHLGETVVYVMLLVSSVAVLSEVYGDLLRAAGATERL
                     MELLASVSPVREPASPRPLPPVGAGSGLELSHVQFNYPSRPEHPTLVDFSLTVRPGET
                     VALVGPSGAGKSTVLQLLLRFYDTQAGAVRVDGVDVRDAALGELRARIGIVPQDSTIF
                     SADALENIRYGRPDATDEEVFAAARAAFADEFIRRLPEGYRTFLGERGVRLSGGQRQR
                     IAIARAMLKNPPLLLLDEATSALDAESERMVQAALEAAMTGRTTLVIAHRLATVQKAD
                     RIVVLEQGRIVEEGTHADLMHRDGLYAKLARMQFHTPQLVHPAAADPRAA"
     gene            2615..3049
                     /locus_tag="VF453_02860"
     CDS             2615..3049
                     /locus_tag="VF453_02860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7686615.1"
                     /translation="MTTPPEDVDALFGAPAPAPSSGLPTAAPAISTSADGRKEQRVKV
                     SWPARVQLPNGRVVDLRARDLSESGVGLLTDVAIPAHTVLNFAMGVPGLHEATKITPV
                     TGTIKTTYLVLQGRDLCCGGMWVNLPEDSRDLLNKWIRRLRK"
     gene            complement(3139..4008)
                     /locus_tag="VF453_02865"
     CDS             complement(3139..4008)
                     /locus_tag="VF453_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX7686616.1"
                     /translation="MDDAILTCALTGVLTDPAQHPVPVTAAQMAAEARDAFNAGASVM
                     HVHVRMQEPGMGHLPSWDPDVAEEVCGAIREACPGVILNLTTGILGKDISGPAACIRR
                     VRPEIAACNAGSLNYLKLRADGRWAWPPLVFDNPVAKVQQFLDVMEEVGAHPEFECFD
                     VGIVRSVDMFVRAGMVKPAMGRPEVNLVMGVASGMPCDADLLALLPRWIPEGASWQAT
                     LIGRDEIWPVHQRVAELGGMLRTGLEDSFYLPGGERAGGNGPMIAALAACAERAGRRV
                     ASPARAREMLGLA"
BASE COUNT          554 a         1552 c         1481 g          603 t
ORIGIN      
        1 gcggcgtcgc cggtctcccg ggcctggggc gccgcttcgg cgcggcgcgg gcattcttgt
       61 tggcggaaag tgcggggcat ggtgtgcttc tcttgtcagt cgggagtggc ggacgcctgg
      121 gcggcgcgca gggcgtcgcc gttggcccgc attcggtgga gcatgcccag gaggctgcgc
      181 cactcggatt cggtgaagcc ggcgaggtgc tggttgagca cgtcggcgag cacggccggc
      241 acgatggccg ccacctcgcg gcccgtctcg gtcagctcca ggttcacgac gcgccgatcc
      301 tcgctggacc gcacccgcgc caccaggccc ttggcctcga ggcggtcgag cgcccgcgtc
      361 atcgcgcccg ggtcgctctg cagcttgcgc gccagctcgg ccacggtcgc gcattcgccg
      421 tgcgccagct tgtacagcgg cacccactgg gcgtgggtca ggtcgtggtc gaccagccgg
      481 cggtcgacct gcgactggat cgaagccagc acgcccttca ggacgctgcc gacgctctcc
      541 tcgcgtcgga aggtctcggg ggcgtagaag ccgggaacgg cagggtgctg cgtcatgatg
      601 ggcgtgaata tacgtgccta ggcagtcatt gtttggacag tgattgaata ggctttaatt
      661 gtgtggggga ttaccctcgg tcaccccgac ggcggtgccg ggggattgcg tagacttcgg
      721 cgccatgacc gccaacaccg accgcgcgaa gggcaccacc ccccgctcgc tgaccgggct
      781 gatgccctac ctgcgtccct accgcggccg gatcgcggcg gcgatcgtct tcctcatcgc
      841 cgcggcggtg accaccctcg ccttcccggt cgcgctgcgc tcgctgatcg acaacggcct
      901 gggcgtgagc gcgacgccgg gcgccagcgc cgcggccctg aacggcgcac gcgtgatggc
      961 gctgcgcgac cacttcctcg ccctgttcgg cgtcggtgcg gcgctgggcg tgttctcggc
     1021 gctgcgcttc tactccgtca cctggctcgg cgagcgcgtg acggccgacc tgcgcacggc
     1081 cgtctacagc cacgtcgtgc gccagagccc cgcgttcttc gagaccacgg ccaccggcga
     1141 ggtgctctcg cgcctgacca cggacacgac gctcgtgcag caggtcgtcg gctcgagcct
     1201 gtccatgggg ctgcgcaacg gcgtgatggg gctcggcgcg ctcgtgatgc tggtcgtgac
     1261 caatccgacc gtgatgtcgc aggtgctggg catcctcgtg ctggtcgtcg cgccgtcgat
     1321 gtacttcggg cgacgcgtgc gcaagctcag ccgcgcgagc caggaccgcg tcgccgactc
     1381 cagcgccatc gcggccgagg tgctcaacgc gatcccggtc gtgcagggct acacgcagga
     1441 gacgcgcgag gccgcgcgct tctcgacgtc gaccgagcac gcgttcgaga ccgccaggaa
     1501 gcgcaccctg gtgcgcgccg tgctcgtcgc cttcatcatc agcgcgacct tcgcggcgct
     1561 gctgtggggc ctgtaccagg gcacgacggc cgtgatgcac ggcgagatga cggccggcca
     1621 cctgggcgag acggtcgtct acgtgatgct gctggtcagc agcgtggcgg tgctgtcgga
     1681 ggtctacggc gacctgctgc gcgcggccgg cgcgaccgag cgcctgatgg agctgctcgc
     1741 ctcggtctcg cccgtgcgcg agccggcgtc cccgcgcccg ctgccgccgg tgggcgcggg
     1801 ctcgggcctc gagctctcgc acgtgcagtt caactacccg tcgcgaccgg agcatcccac
     1861 gctcgtcgac ttctcgctca cggtgaggcc cggcgagacg gtggccctcg tcgggccgag
     1921 cggcgccggc aagagcaccg tgctgcagtt gctgctgcgc ttctacgaca cccaggccgg
     1981 cgccgtgcgc gtcgacggcg tggacgtgcg cgacgccgcg ctcggcgagc tgcgcgcgcg
     2041 catcggcatc gtgccgcagg acagcacgat cttctcggca gacgccctcg agaacatccg
     2101 ctacggccgc ccggacgcca cggacgagga ggtcttcgcc gcggcgcgcg cggccttcgc
     2161 cgacgagttc atccgccgcc tgcccgaggg ctaccgcacc ttcctgggcg agcgcggcgt
     2221 gcgcctgtcg ggcggccagc gccagcgcat cgcgatcgcg cgcgcgatgc tgaagaaccc
     2281 gccgctgctg ctgctcgacg aagccaccag cgccctcgac gcggagagcg aacgcatggt
     2341 gcaggccgcg ctcgaggccg cgatgaccgg acgtacgacg ctggtcatcg cccaccggct
     2401 cgcgaccgtg cagaaggcgg accgcatcgt cgtgctggag caaggccgca tcgtcgaaga
     2461 gggcacgcat gccgacctga tgcaccgcga cgggctgtac gcgaaacttg ccagaatgca
     2521 gttccatacg ccgcagctcg tccatcccgc ggccgccgac ccgcgcgccg cctgagcgcg
     2581 cgctccgatc ccgattccga tccgcagcct cgccatgacg actccccctg aagacgtcga
     2641 tgccctgttc ggcgccccgg cgcccgcgcc ctccagcggc ctgccgaccg ccgccccggc
     2701 gatcagcacc tcggccgacg gccgcaagga gcagcgcgtc aaggtctcgt ggcccgcgcg
     2761 cgtgcaactg cccaacggcc gcgtcgtcga cctgcgcgcg cgcgacctct ccgagagcgg
     2821 cgtcggcctg ctcaccgacg tcgcgatccc cgcgcacacc gtgctgaact tcgcgatggg
     2881 cgtgcccggc ctgcacgagg ccacgaagat cacgcccgtg accggcacga tcaagacgac
     2941 ctacctcgtg ctgcagggcc gcgacctgtg ctgcggcggc atgtgggtga acctgcccga
     3001 ggactcgcgc gacctgttga acaagtggat ccggcggctg cgcaagtagc gcggggccgc
     3061 ggcctcatcc ccgacggccg tcctcgcgcc ggcggcgcac cgccggctgc ccgcgcgtga
     3121 cgcgggacag gcgcgacttc aggcgaggcc gagcatctcc cgcgcccgag ccggactcgc
     3181 gacccgcctc cccgcccgct ccgcgcaggc cgcgagggcc gcgatcatcg ggccgttgcc
     3241 gcccgcccgc tcgccgccgg gcaggtagaa gctgtcctcc aggccggtgc gcagcatgcc
     3301 gccgagctcg gcgacgcgct ggtgcaccgg ccagatctcg tcgcggccga tcagcgtggc
     3361 ctgccagctg gcgccctcgg ggatccagcg cggcagcagc gccagaaggt cggcgtcgca
     3421 cggcatgccc gaggcgacgc ccatcacgag gttgacctcc ggccgcccca tcgccggctt
     3481 gaccatgccg gcgcggacga acatgtcgac cgagcgcacg atgccgacgt cgaagcactc
     3541 gaactcgggg tgcgcgccga cctcctccat cacgtcgagg aactgctgca ccttggcgac
     3601 cgggttgtcg aacaccagcg gcggccaggc ccagcggccg tccgcgcgca gcttgaggta
     3661 gttgaggctg ccggcgttgc aggccgcgat ctcgggccgg acgcggcgga tgcaggccgc
     3721 cggaccggag atgtccttgc cgaggatgcc cgtcgtcagg ttgaggatca cgccggggca
     3781 ggcctcgcgg atcgcgccgc agacctcctc ggccacgtcc ggatcccacg acggcaggtg
     3841 ccccatgccg ggctcctgca tgcgcacgtg cacgtgcatg acggaggcgc ccgcgttgaa
     3901 ggcgtcgcgc gcctcggcgg ccatctgcgc cgcggtgacg ggcaccgggt gctgcgcggg
     3961 atcggtgagc acgccggtca gcgcgcaggt caggatcgcg tcgtccatcg gagcttcctt
     4021 cgtggttctt gctgcggccg tcagtcggaa ggcccgtcga ggtcgagctc gaccagcacc
     4081 gtgcccgccg cgaccgtgtc gccggccgcg gcccgcagcg cggccaccgt gccggcggcg
     4141 ccggcgtgca gccacatctc catcttcatc gcctcgacgc aggcgagcgg
//
LOCUS       DASVLN010000130         5214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_328667, whole genome shotgun sequence.
ACCESSION   DASVLN010000130 DASVLN010000000
VERSION     DASVLN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328667"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..124
                     /locus_tag="VF453_02870"
     CDS             <1..124
                     /locus_tag="VF453_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HEX7686617.1"
                     /translation="TGPVTLASGPRIGITKAADLPWRFGLAGSVHLSRPFRVAR"
     gene            146..361
                     /locus_tag="VF453_02875"
     CDS             146..361
                     /locus_tag="VF453_02875"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7686618.1"
                     /translation="MRRNMASVEAELEPAGLRRIHRTAIVNLARVRALGAGVDGETEV
                     VLRDGRRLPTSRRHRTALQERLGLRGA"
     gene            complement(363..1148)
                     /locus_tag="VF453_02880"
     CDS             complement(363..1148)
                     /locus_tag="VF453_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686619.1"
                     /translation="MFDLLAKQINSSAINGIRLFDIDPADLHKASLDFVRDGYVLVKQ
                     ALTPEFCEFCESTIKFDERVRSDSRQFYRDHNLKDGMSDVLVEMFHSLSVPLFAAIAG
                     MTVKKQYSFAMKYVAGSNLLPHWDLDLNSISATVCYKGSSQSNPIYIDRARFLNPYVN
                     RVTIKDKGSIPRANVARIDVEHRDIGIFRGREHLHWRDLIEFEEDYRAMLIHFTDYNL
                     NGVSMDPPKRDQIEGIRAALIDCEDYMEFRQRYCMYFDNRTGY"
     gene            complement(1266..2186)
                     /gene="bla"
                     /locus_tag="VF453_02885"
     CDS             complement(1266..2186)
                     /gene="bla"
                     /locus_tag="VF453_02885"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HEX7686620.1"
                     /translation="MQRRHFNAGLALVLGLAAARATGQKMLGDAASGPDGDGAWAAIE
                     SAVGGRLGVAMLCTGSGKLAGHRLDERFPMCSTFKWLLAACVLRRVADGQERLDRRLV
                     YAGRKLPEWSPVSGKHADANGLTIGELCQAAIALSDNGAANLLLETVGGPQAVTAQAR
                     AFGDGQTRLDRHEPELNVVPPGDARDTTTPRAMAGALRTGLLGDGLPPAQRAQLVAWM
                     SGTQTGLKRLRAGVPAGWRVADKTGTWDKSTLNDVAVFWPPDRPPVVVAAYLTDCPPR
                     PGIDREHALADVGREVARRVQAASRPGKAS"
     gene            2300..3166
                     /locus_tag="VF453_02890"
     CDS             2300..3166
                     /locus_tag="VF453_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7686621.1"
                     /translation="MTLPLNALRAFEASARHLSFTRAAMELHVTQNAVSAQVKNLETR
                     LGVPLFRRLPRGLALTDEGVALLPALSDSFERMRGVLEQLEGGLKVEVLTVAVVGTFA
                     VGWLLPRLEAFQRDQPSVDLRLLTNNNRVDLAAEGLDYAIRFGDGAWHGTEAEPLLEA
                     PLSPVCSPRIAARLASPADLAREKLLRSYRADEWPRWFSAAGCTTPAPIARGAIFDSS
                     ITLADAAAQGAGVALLPVAMFGEALRTERLMQPFALGVSLGRYWLTRLKSRPVTQAMR
                     TFREWLLAESAG"
     gene            complement(3556..4563)
                     /locus_tag="VF453_02895"
     CDS             complement(3556..4563)
                     /locus_tag="VF453_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7686622.1"
                     /translation="MTTIKDVARLAGVGVGTASRVISGKGSFSQGAALRVETAAKQLG
                     FRPSAIARALSLQSTGTIGIFVPDFKGPFYGPLLDAIDAELRAQDRHMVAANGCGDAD
                     SRQQSIDAVRFLIDRECDGIVICSNTLRDADFLQIRASFPNIAVVNRDVKGMKSQCFT
                     VDHKAAGRIAADTLLAHGHRRIAVISGPDVGDNRQRLQGLYERLAEDGVDTAAIPVEE
                     GDFSAPSGWDAAERLIARKPDVTAVFCANDQMAMGAISCMQSRGIAIPRKWSVMGYDD
                     ADVAAWLSPRLTSVHIPIADMGLNACRMLINTRHGTSLPVSRKFVPEVVMRDSLARVR
                     A"
     gene            complement(4609..>5214)
                     /locus_tag="VF453_02900"
     CDS             complement(4609..>5214)
                     /locus_tag="VF453_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX7686623.1"
                     /translation="VREAQVFLFDEPLSNLDAKLRTELRRELKGLHRELGATMIYVTH
                     DQVEAMTLATRVAVMRGGRILQVGTPGEVYARPVDIFVAGFLGSPGMNFIEGQAVAAA
                     GAAPMPVVLGVRPEHVELQPPGDAFPAAEVTLVEALGAHELVWLDMQGHKLSALVPAQ
                     GRYAPGQAVGVRMDLERASVFDPRTGHRLELHASAFYHRPH"
BASE COUNT          724 a         1782 c         1909 g          799 t
ORIGIN      
        1 caccgggccg gtgacgctcg cgagcggccc gcgcatcggc atcacgaagg cggccgacct
       61 gccgtggcgc ttcgggctcg ccggctcggt gcacctgagc cggccgttcc gcgtggcgcg
      121 ctgacgcctc gattcgcggc acacgctgcg ccggaacatg gcctcggtcg aggccgagct
      181 cgagcccgcg ggcttgcgcc gcatccaccg caccgcgatc gtcaacctcg cgcgcgtgcg
      241 cgcgctgggg gccggcgtcg acggcgagac cgaggtcgtg ctgcgcgacg gacggcgcct
      301 gccgacgagc cgacgccacc gcacggcgct gcaggagcgg ctcggattgc gcggcgcgtg
      361 actcaataac ccgtgcggtt gtcgaaatac atgcagtagc gctggcggaa ctccatgtag
      421 tcctcgcagt cgatcagcgc cgcccggatg ccctcgatct ggtcgcgttt cgggggatcc
      481 atcgacacgc cgttgaggtt gtagtccgtg aagtggatca gcatcgcgcg gtagtcctcc
      541 tcgaactcga tgaggtcgcg ccaatgcagg tgctcgcggc cgcggaagat gccgatgtcg
      601 cggtgctcga cgtcgatgcg cgcgacattg gcgcgcggaa tcgagccctt gtccttgatc
      661 gtcacgcggt tcacgtacgg gttcaggaag cgcgcgcggt cgatgtagat cgggttgctc
      721 tggctcgagc ccttgtagca gaccgtggcc gagatcgagt tgaggtcgag gtcccagtgc
      781 ggcagcaggt tggagccggc gacgtacttc atcgcgaagg agtactgctt cttgacggtc
      841 atgccggcga tcgccgcgaa cagcggcacc gacagcgagt ggaacatctc gaccagtacg
      901 tcggacatgc cgtccttgag attgtggtcg cggtagaact ggcgcgagtc ggaacgcacg
      961 cgctcgtcga acttgatcgt cgattcgcag aactcgcaga actcgggtgt cagcgcctgc
     1021 ttgacgagca cgtagccgtc gcgcacgaag tcgaggctcg ccttgtgcag gtcggcgggg
     1081 tcgatgtcga acaggcggat gccgttgatg gcggaggagt tgatctgctt ggccaggaga
     1141 tcgaacatgc ggagggctcc agggcgtgcg tggacgcggc ttcgtgatgt cggccagcgt
     1201 aacggccgcc gcgcgcggcc aagcccggat cagcggcggc aatcgccgcc tgttcgtccc
     1261 gtcggtcagc tcgccttgcc cggccgggac gccgcctgca cgcgccgggc gacctcgcgt
     1321 ccgacgtcgg ccagcgcgtg ctcgcggtcg atgcccggtc gcggcgggca gtcggtgaga
     1381 taggccgcga cgacgacggg cgggcgatcc ggcggccaga acaccgcgac gtcgttgagc
     1441 gtggacttgt cccaggtgcc agtcttgtcg gcgacgcgcc agccggccgg cacgcccgcg
     1501 cgcaggcgct tcaggccggt ctgcgtgccc gacatccaag cgacgagctg cgcgcgctgc
     1561 gccggcggca ggccgtcgcc caggaggccc gtgcgcaagg cgccggccat cgcgcgcggg
     1621 gtcgtggtgt cgcgcgcgtc gcccggcggc acgacgttga gctcgggctc gtggcggtcg
     1681 aggcgggtct ggccgtcgcc gaaggcgcgc gcctgcgcgg tcacggcctg cgggccgccc
     1741 acggtctcca gcagcaggtt ggcggcgccg ttgtcgctga gggcgatcgc ggcctggcag
     1801 agctcgccga tcgtcaggcc gttggcgtcc gcgtgcttgc ccgacaccgg cgaccactcc
     1861 ggcagcttgc ggccggcgta gacgagccgc cggtcgaggc gctcctgccc gtcggccacg
     1921 cgccgcagca cgcacgcggc gagcagccac ttgaaggtgc tgcacatcgg gaagcgctcg
     1981 tcgagccggt ggccggcgag cttgccgctg cccgtgcaca gcatcgccac gcccaggcgg
     2041 ccgccgaccg ccgattcgat ggcggcccag gcgccgtcgc catccgggcc gctcgcggcg
     2101 tccccgagca ttttctggcc ggtcgcccgc gccgccgcga gcccgagcac gagcgccagg
     2161 cccgcgttga agtgtcgtct ttgcatgtcg ttccctttgg aagccggctt gtccgccgtg
     2221 gatcgctgcg cacgatagcg gcgccgcggc gcgggctcaa acgctgattt cgagggactg
     2281 gccgaagata atctggggca tgacgcttcc gctgaacgcc ctgcgcgcct tcgaggcctc
     2341 ggcccgccac ctgagcttca cgcgcgccgc gatggaactc cacgtgacgc agaacgccgt
     2401 cagcgcgcag gtcaagaacc tcgagacgcg gctgggcgtg cccttgttca ggcgcctgcc
     2461 gcgcggtctc gcgctgaccg acgagggcgt cgcactgctg ccggcgctca gcgacagctt
     2521 cgagcgcatg cgcggcgtgc tggagcagct cgagggcggg ctgaaggtcg aggtgctgac
     2581 ggtggcggtc gtcggcacct tcgcggtcgg ctggctgctg ccgcggctgg aggccttcca
     2641 gcgcgaccag ccttcggtcg accttcgcct gctgacgaac aacaaccgcg tcgacctcgc
     2701 cgccgaaggg ctggactacg cgatccgctt cggcgacggc gcctggcacg gcacggaggc
     2761 ggagccgctg ctggaggcgc cgctgtcgcc ggtctgcagc ccgcgcatcg ccgcgcgcct
     2821 ggcgtcgccc gccgacctcg cgcgcgagaa gctgctgcgc tcctaccgcg ccgacgagtg
     2881 gccgcgctgg ttctccgcgg ccggctgcac gacgcccgcg ccgatcgcgc gcggggcgat
     2941 cttcgactcc tcgatcacgc tggccgacgc cgccgcgcag ggcgccggcg tcgcgctgct
     3001 gccggtggcg atgttcggcg aggcgctgcg caccgagcgc ctgatgcagc cgttcgcgct
     3061 gggcgtctcg ctcgggcgct actggctcac gcgcctgaag tcgcggccgg tgacgcaggc
     3121 gatgcggacg ttccgggagt ggctgctggc cgaatcggcg ggctagtcaa agcgcgcgag
     3181 ccacgattcc acgccggatc gaatgaattc gactccggat gcaatgaacg caccattccg
     3241 gcgagctgtc gatgccgcgc atcgaatgaa ttcagcgcgg aacggaatga attcaatgcg
     3301 cagggaaacg agttcaatcc cgagatcaat caattccatc cggagatgag tgaattcacc
     3361 gccggggtga gttcattgga ttcggcattg aattcattgg atgcggcgac taattcatgc
     3421 accggcctgg ggtgttcatt catctccggg aagaattcaa tcggccggtg aacttccccg
     3481 cggatccgcg cgctccgtgg tcggagacgg ctgcccggat ggcggccgcg gatcggcgcc
     3541 ctcgcggagc gcgtctcagg cgcgcacgcg cgcgagcgaa tcgcgcatca cgacctcggg
     3601 cacgaacttg cgcgagaccg gcaggctggt gccgtggcgg gtgttgatca gcatgcggca
     3661 ggcgttcagg cccatgtcgg cgatggggat gtgcacgctg gtcaggcgcg gcgagagcca
     3721 ggccgcgacg tcggcatcgt cgtagcccat caccgaccac ttgcgcggga tcgcgatgcc
     3781 gcgcgactgc atgcagctga tcgcgcccat ggccatctgg tcgttggcgc agaagacggc
     3841 cgtgacgtcg ggcttgcgcg cgatgaggcg ctcggccgcg tcccagcccg agggggcgct
     3901 gaagtcgcct tcctcgaccg ggatcgcggc ggtgtccacg ccgtcctcgg ccaggcgctc
     3961 gtacaggccc tgcaggcgct ggcggttgtc gcccacgtcg gggccggaga tcacggcgat
     4021 gcgccggtgg ccgtgggcga gcagcgtgtc cgccgcgatg cggcccgccg ccttgtggtc
     4081 gacggtgaag cactgcgact tcatcccctt gacgtcgcgg ttgacgacgg cgatgttggg
     4141 gaagctcgcg cggatctgca ggaagtcggc gtcgcgcagc gtgttgctgc agatgacgat
     4201 gccgtcgcac tcgcggtcga tcaggaagcg cacggcgtcg atcgactgct ggcgcgagtc
     4261 ggcgtcgccg cagccgttgg ccgcgaccat gtggcggtcc tgcgcgcgca gctcggcgtc
     4321 gatggcgtcc agcagcgggc cgtagaacgg gcccttgaag tcggggacga agatgccgat
     4381 cgtgccggtg ctctgcagcg acagcgcgcg cgcgatggcg ctcgggcgga agcccagctg
     4441 cttggccgcg gtctccacgc gcagcgccgc gccctgggag aacgagccct tgccgctgat
     4501 cacgcgcgag gcggtgccga cccccacgcc ggcgagccgt gctacgtcct tgatcgtcgt
     4561 catcgtggtc gggcctctcg cgtcgtcgtt tgcctgcctg gcgggcgctc agtggggccg
     4621 atgatagaac gccgacgcgt gcagctcgag ccggtgcccg gtgcgcgggt cgaagacgct
     4681 cgcgcgctcg aggtccatgc gcacgccgac cgcctggccc ggcgcgtagc ggccctgcgc
     4741 gggcacgagc gccgacagct tgtggccctg catgtccagc cacacgagct cgtgcgcgcc
     4801 gagcgcctcg accagggtga cctccgccgc cgggaaggcg tcgccgggcg gctgcagctc
     4861 gacgtgctcg ggccgcacgc ccagcaccac gggcatgggc gcggcgcccg cggcggcgac
     4921 ggcctggccc tcgatgaagt tcatgcccgg cgacccgagg aatccggcga cgaagatgtc
     4981 caccggccgc gcgtagacct cgcccggcgt gccgacctgc aggatgcggc cgccgcgcat
     5041 caccgcgacg cgcgtggcga gcgtcatcgc ctcgacctgg tcgtgggtca cgtagatcat
     5101 cgtggcaccg agctcccggt gcaggccctt gagctcgcgc cgcagctcgg tgcgcagctt
     5161 ggcgtcgagg ttggacaggg gctcgtcgaa gaggaagacc tgcgcctcgc gcac
//
LOCUS       DASVLN010000131         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_168281, whole genome shotgun sequence.
ACCESSION   DASVLN010000131 DASVLN010000000
VERSION     DASVLN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168281"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1343)
                     /locus_tag="VF453_02905"
     CDS             complement(261..1343)
                     /locus_tag="VF453_02905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7686624.1"
                     /translation="MTASPSEERFLPAAGAALAPASHSALDVDGVPFHRLSVVVPMYN
                     EVENAAALVDEVQAALGDYPFPWELIVVDDGSRDGTPAALEKRGAELGPHIRVIRLWR
                     NFRQTAAMQAGIDASRGDVIVTMDGDLQNDPRDIPKLVARLLTGDLDFVAGWRKNRQD
                     GFWLRKFPSRIANALIRKSTGLQFNDLGCSLKAFRGSVLREVRLYGEMHRFIPAWLAT
                     VTSPARMDEVPVNHRARVAGTSKYGLSRTFRVITDLLAMHFFLNYGTRPGHFFGGIGL
                     AAGGVGGLILAWMAVCKFVLGQAIGGRPLLALGFFLVIGGLQLLLTGVLAELLIRIYY
                     DGRHAQPYHHGRAAAPGEDAAWHTVR"
BASE COUNT          218 a          503 c          593 g          189 t
ORIGIN      
        1 ggcggcccgg gcttgcgcac cggcggatcg agcggcatcg gcgccgaggc gacgttgaac
       61 gtcagctcgg ggggcggctc cggctcggcg gactcgctgc cgagcaggtt gtcgtacggc
      121 atcagcggca gcggctccgc agcgggcggc ggcgccggcg ggcgcgggcc cggaccgggg
      181 gccggcgacg aaggcgaggg cggagtcgga ggctgggcca tcggaggtcg gctgtgcggg
      241 gcgattcgtt tcgcgggtct tcagcgcacc gtgtgccagg cggcgtcctc gcccggcgcg
      301 gcggcgcgcc cgtgatggta gggctgcgcg tggcgcccgt catagtagat gcggatgagc
      361 agctcggcca gcactcccgt cagcagcagt tgcagcccgc cgatgacgag gaagaagccg
      421 agcgccagca gcggccggcc gccgatcgcc tgcccgagaa cgaacttgca caccgccatc
      481 caggccagga tcagtccccc cacgccgccg gcggcgagcc cgatgccgcc gaagaagtgc
      541 ccgggccgcg tgccgtagtt gaggaagaag tgcatcgcca gcaggtcggt gatcacgcgg
      601 aaggtgcgcg acaggccgta cttgctggtg cccgccacgc gggcgcgatg gttgaccggg
      661 acctcgtcca tgcgcgccgg cgaggtcacc gtcgcgagcc aggccgggat gaagcggtgc
      721 atctcgccgt acagccgcac ctcgcgcagc accgagccgc ggaacgcctt caggctgcag
      781 ccgaggtcgt tgaactgcag gccggtcgac ttgcggatca gcgcgttggc gatgcgcgag
      841 gggaacttgc gcagccagaa gccgtcctgg cggttcttgc gccagccggc gacgaagtcg
      901 aggtcgccgg tcagcaggcg ggcgacgagc ttggggatgt cgcgggggtc gttctgcagg
      961 tcgccgtcca tggtgacgat gacgtcgccg cgcgaggcgt cgatgccggc ctgcatcgcc
     1021 gcggtctggc ggaagttgcg ccacaggcgg atcacgcgga tgtggggccc gagctccgcg
     1081 ccgcgcttct cgagcgccgc cggggtgccg tcgcggctgc cgtcgtcgac gacgatcagc
     1141 tcccacggga acgggtagtc gccgagcgcg gcctgcacct cgtccaccag cgcggcggca
     1201 ttctccacct cgttgtacat cggcacgacg accgagaggc ggtggaacgg gacgccgtcg
     1261 acgtcgagcg cgctgtgcga ggccggcgcg agcgcggcgc ccgcggcggg aaggaagcgt
     1321 tcctcggaag gactcgcggt catgtcggac tttctggcgt ccgcgacgga cgcacgatca
     1381 gggagaaggc gagcgcggtg gccagcgcca ccacgatcat cagcgcgtgc accgcgagcg
     1441 cggcgccaaa cagcaaccgc gcattctcgc ggaccggcgc gggggccggg gcgccgggcg
     1501 cgg
//
LOCUS       DASVLN010000132         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_436463, whole genome shotgun sequence.
ACCESSION   DASVLN010000132 DASVLN010000000
VERSION     DASVLN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436463"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF453_02910"
     CDS             <1..204
                     /locus_tag="VF453_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686625.1"
                     /translation="EPGTVRRLGDRARAVATHRFGLPRLVDELETCLRGLAVERVVRA
                     ELFPRARPHPATPLALTLAKDPR"
     gene            201..1343
                     /locus_tag="VF453_02915"
     CDS             201..1343
                     /locus_tag="VF453_02915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7686626.1"
                     /translation="MIVILYPQFHRIGGIARYLDAFLSNLPAGSPPVVLVTGDPPAAE
                     WSRPGVRCIAIPVPGNRLGLALWGWKAAREVARLAREAPVTAVNLHMPPLIPGLFLPP
                     GLRLVLTAHTTYLGMSGRFEGNAHFRSPWNALAVRLKMLMERAVIARADTVVTLTEQG
                     RDELARYGRTEGVEVVPNGVDLAEFTDDAAVARDIDVIFCGRIELRKGSRPMVEVCRR
                     LVQADPAIRIAIVGYGDDEAHVWKALAPLGDNVLLTGKVSFGDMVSYYRRSRLYASTS
                     YYEGLPGTCLEAMAMRLPPVVWDRAFYRHLVVHGISGRIVRVNDVEAMAGEILRLLRR
                     DEERQALGDNARAIVRDRYDWRRLAARLVQIDERAARVRAVQEATT"
     gene            1340..>2471
                     /locus_tag="VF453_02920"
     CDS             1340..>2471
                     /locus_tag="VF453_02920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7686627.1"
                     /translation="MTTTGIEAHAAPRGARHPRVMWTGLRGFPNVQGGVETHAEHMCP
                     RLEELGVDVTVLARRCYQPAEVGARWHGVEFVRVWAPRRKQLEAIVHTFLSVLYAGFV
                     SRPDVLHIQAIGPAIWTPLARLLGLRVVVTHHGPDYDRQKWGRLAKFVLRLGEAAAAR
                     WASELIVISRVIQRSVAIEHGRVGVLVPNGLDLPGESPDASALPAFGLEPGRYVLLVS
                     RMVPEKRHLDLVRAFALAAIPGWKLALVGAADHVDAYAREVAQVAGGTPGVVMTGFQT
                     GAALQALYAHAGLFVLPSSHEGLPIAMLEALSHGVPVVASDILPNLEVGLPRDCHFPL
                     GDVEALARLLRERAARAETPAERDARRAWAAKRFDWTRNARAT"
BASE COUNT          302 a          916 c          903 g          350 t
ORIGIN      
        1 gagcccggca cggtgcgccg cctcggcgat cgggcgcgcg cggtcgcgac gcaccggttc
       61 gggctgccgc ggctggtcga cgagctcgag acctgcctgc gcggactcgc cgtggagcgg
      121 gtggtgcggg ccgagctgtt cccccgcgcg cgcccgcacc ccgccacgcc gctcgcactg
      181 acccttgcca aggatccccg atgatcgtca tcctctatcc ccagttccac cgcatcggcg
      241 gcatcgcccg ctatctcgac gcgttcctgt cgaacctgcc cgcgggctcg ccgccggtcg
      301 tgctggtgac cggcgacccg ccggcggccg agtggtcccg ccccggcgtg cgctgcatcg
      361 ccatcccggt gcccggcaac cgcctcggcc tggcgctgtg gggctggaag gcggcgcgcg
      421 aggtggcgcg gctggcgcgc gaggccccgg tcacggccgt gaacctgcac atgccgccgc
      481 tgatcccggg acttttcctg ccgcccggcc tgcggctcgt gctcaccgcg cacacgacct
      541 acctgggcat gtccggacga ttcgagggca acgcgcattt ccgcagccct tggaacgcgc
      601 tggcggtgcg gctgaagatg ctgatggagc gcgccgtcat cgctcgcgcg gacacggtcg
      661 tcacgctgac cgagcagggc cgcgacgagc tcgcccgcta cggccgtacg gagggcgtcg
      721 aggtcgtgcc caacggcgtc gacctcgccg agttcaccga cgacgcagcc gtcgcgcgcg
      781 acatcgacgt gatcttctgc ggccgcatcg agctgcgcaa gggcagccgc ccgatggtcg
      841 aggtctgccg ccgcctcgtg caggccgacc ccgccatccg catcgccatc gtcggctacg
      901 gcgacgacga ggcgcacgtg tggaaggcgc tcgcgccgct gggcgacaac gtgctgctga
      961 cgggcaaggt ctcgttcggg gacatggtgt cctactaccg ccgcagccgc ctgtacgcct
     1021 cgacgtccta ctacgaaggc ctgcccggca cctgcctgga ggcgatggcg atgcgcctgc
     1081 cgcccgtcgt ctgggatcgc gcgttctacc gccatctcgt cgtccacggc atcagcggac
     1141 gcatcgtgcg cgtgaacgac gtcgaggcga tggccggcga gatcctgcgc ctgctccggc
     1201 gcgacgagga gcgccaggcg ctcggggaca acgcgcgcgc catcgtgcgc gaccgctacg
     1261 actggcgccg gctggcggcg cggctcgtgc agatcgacga acgcgccgcc cgcgtccggg
     1321 cggtgcagga ggccacgaca tgacgacgac gggcatcgag gcgcatgccg cgccgcgcgg
     1381 cgcgcggcat ccgcgcgtca tgtggaccgg cctgcgcggc tttcccaacg tgcagggcgg
     1441 cgtcgagacc catgcggaac acatgtgccc gcggctggag gaactcggcg tcgacgtgac
     1501 cgtgctggcg cggcgctgct accagccggc cgaggtgggc gcgcgctggc acggcgtgga
     1561 gttcgtgcgc gtctgggcgc cgcgccgcaa gcagctcgag gcgatcgtgc acaccttcct
     1621 gtcggtgctg tatgccggct tcgtgtcgcg gccggacgtg ctgcacatcc aggccatcgg
     1681 cccggcgatc tggacgccgc tcgcgcggct gctcggattg cgggtcgtcg tcacccacca
     1741 cgggccggac tacgaccgcc agaaatgggg ccggttggcg aagttcgtgc tgcgcctggg
     1801 cgaggcggcg gccgcgcgct gggcgagcga gctcatcgtg atctcgcgcg tgatccagcg
     1861 cagcgtcgcg atcgagcatg ggcgcgtggg ggtgctcgtg cccaacgggc tcgacctccc
     1921 cggcgagagc cccgacgcct ccgcgctccc agccttcggg ctcgagccgg gccgctacgt
     1981 gctgctcgtg agccgcatgg tgcctgagaa gcgccacctc gacctcgtcc gtgccttcgc
     2041 gctggccgcg atcccgggct ggaagctcgc gctcgtgggc gcggcggatc atgtggacgc
     2101 ctacgcccgc gaggtcgcgc aggtggccgg gggcacgccg ggcgtcgtga tgaccggctt
     2161 ccagaccggc gcggcgctgc aggcgctgta cgcgcacgcc ggcctgttcg tgctgccgtc
     2221 ctcgcacgag gggctgccca tcgcgatgct ggaggcgttg tcgcacggcg tgccggtcgt
     2281 cgcgagcgac atcctgccca acctcgaggt cggcctgccg cgcgactgcc acttcccgct
     2341 cggcgacgtc gaggcgctgg cccggctgct gcgcgagcgc gcagcccgcg ccgagacccc
     2401 ggccgaacgc gacgcgcgcc gggcgtgggc cgccaagcgc ttcgactgga cgcgcaacgc
     2461 gcgcgcgacg a
//
LOCUS       DASVLN010000133         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_490005, whole genome shotgun sequence.
ACCESSION   DASVLN010000133 DASVLN010000000
VERSION     DASVLN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490005"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VF453_02925"
     CDS             <1..754
                     /locus_tag="VF453_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7686628.1"
                     /translation="AAAARRGRPGLDVVIDLASAAWMWLDFLGAAVLALAATVLARPT
                     SRRRVVRAIAARGLAIAGIELECTGQDVLADGGRGGVYVCNHASYVDWLVLVAALPVH
                     ARFVAKRELARRRPLRWFLARMGVHFVERVEVRARTADAAALARLMAEGQCLVMFPEG
                     TFGRAPGLRPFHLGAFMAAARAGVPVVPVGVSGTRSALRDGSWMPRRGRVGVHIDAPL
                     RPTGTGWSDAVRLRDDARACIARHCGEPLARR"
     gene            863..1120
                     /locus_tag="VF453_02930"
     CDS             863..1120
                     /locus_tag="VF453_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686629.1"
                     /translation="MSTSPPLHQVGAPDAERETSPVVDQGDEGDELDIEAGAHGGVCR
                     FNGTPFPVGAMVLSGSEVLRCEAPGVWVRTGELRPHGKVLD"
     gene            complement(1203..1448)
                     /locus_tag="VF453_02935"
     CDS             complement(1203..1448)
                     /locus_tag="VF453_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686630.1"
                     /translation="MDVVLDPELFEAIEAGDTARMERWLVAEGDRVDTGELIGQARAA
                     RELVGILAPHGRILEQILVAAGERFVPGHALARIVEF"
BASE COUNT          190 a          631 c          610 g          211 t
ORIGIN      
        1 cgccgccgcg gcccggcgcg ggcgccccgg cctcgacgtc gtgatcgacc tggcctcggc
       61 agcctggatg tggctggact tcctcggcgc cgccgtgctc gccctcgcgg cgaccgtcct
      121 tgcgcggccg acgtcgcgca ggcgcgtggt gcgggccatc gccgcccgcg ggctggcgat
      181 cgcgggcatc gaactcgaat gcaccgggca ggacgtcctg gccgacggag gccgaggcgg
      241 cgtctacgtg tgcaatcacg cgagctacgt cgactggctc gtcctggtcg cggccctgcc
      301 cgtccatgcc cggttcgtgg ccaagcgcga gctcgcccgg cggcgcccgc tgcggtggtt
      361 cctcgcgcgc atgggcgttc atttcgtcga gcgcgtcgaa gtgcgcgcgc gcaccgcgga
      421 cgccgccgcc ctggcgcggc tgatggccga ggggcagtgc ctggtcatgt tcccggaagg
      481 gacgttcgga cgggcgccgg gcctgcgccc attccacctc ggcgccttca tggccgccgc
      541 gcgggcgggc gtgccggtcg tgccggtcgg cgtgagcggc acgcgatcgg cgctgcgcga
      601 cggctcgtgg atgccgcggc gcggccgtgt cggcgtgcac atcgacgccc cgctgcggcc
      661 gaccggcacc gggtggtccg acgccgtccg gctgcgagac gatgcgcgcg cctgcatcgc
      721 gcggcactgc ggcgaacccc tcgcgcgacg atgatcggcc tcacggcgga cgagaccgtt
      781 gcacgccgtc gttgaggaca cgcaatcgcc gcccggtcgc ggtcgctagc gtcgagatct
      841 ctccccaacg ctccgcgacg ccatgagcac gtcccctccc ctgcaccagg tcggcgcgcc
      901 cgatgccgag cgcgagacct cgcccgtggt cgaccaaggc gacgagggcg acgagctgga
      961 catcgaggcc ggcgcgcacg gcggcgtgtg ccgcttcaac gggacgccgt tcccggtcgg
     1021 cgccatggtg ctcagcggca gcgaggtgct tcgttgcgaa gcgccgggcg tgtgggtgcg
     1081 caccggggag ctccggccgc acggcaaggt gctcgactga ccggcccgcc agggcgcggc
     1141 cctcgccggc gccggaagct cgccgcacac cgaacccgcg gcggccgaca ggcccctccg
     1201 ccctagaact cgacgatgcg tgccagggca tggcccggga cgaagcgctc gcccgcggcc
     1261 accaggatct gctcgaggat ccggccgtgc ggcgccagga tgccgaccag ctcgcgcgcg
     1321 gcgcgcgcct ggccgatcag ctcgcccgtg tcgacccggt cgccttccgc caccagccag
     1381 cgctccatcc gcgccgtgtc gccggcttcg atggcttcga acagctcggg atcgaggacc
     1441 acgtccatgg ggtactccct tcgtcacgcg accgcgcggg ccgccggcaa gacggccgtg
     1501 acgggacgcg ccccgcccct gcgccgcgtg gggccaccgc ggtccgggcc ctgctcccgc
     1561 agatccggcc gaacggcagg aggacgagcc tgacgcggcc gtcggggccg ctcgggccga
     1621 gggaggcagc ccgatctcgt ct
//
LOCUS       DASVLN010000134         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_6960, whole genome shotgun sequence.
ACCESSION   DASVLN010000134 DASVLN010000000
VERSION     DASVLN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6960"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VF453_02940"
     CDS             <1..760
                     /locus_tag="VF453_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686631.1"
                     /translation="AAIASFGRLGRASVASADRHAAGSSTSSGNCGVSGTITTTLNDN
                     DNSSSVSVGDSATIVYNACHDSATSSVSGTISVTITSVSGNSFSATANVQNVQATDQG
                     LSSTVNGQFTLSDDETDTQDDETLAIGSGGLTVSVQCTSYTDTLTFASGMHIETVYDS
                     VSGDTRLSLNGGLSSSKLAGSLTLTTVQPVVQHATDAYPSSGQVRAVGANGSALLMTV
                     LNTTQVQLQLDADGNGSYEASSTANWSTLIGQQG"
     gene            954..>1581
                     /locus_tag="VF453_02945"
     CDS             954..>1581
                     /locus_tag="VF453_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication/partition related protein"
                     /protein_id="HEX7686632.1"
                     /translation="MNIVVNEDLKHYIDPLTPEEHEALERSLLAEGCRDALVLWGDVL
                     VDGHNRHGICMKHGIPFQTVQNTRFRSIEDVHLWMIDQHLGRRSVSDFQRGVLALRKK
                     EILEARQAAARDAERVAEVRGEARPVADDPDALIGGVPIAAAEPLKTREAVARAAKLS
                     SAQVGMIEKIQKQAAPEVVAALKSGTISISAAAAVASLPVDEQVAAASA"
BASE COUNT          260 a          575 c          546 g          200 t
ORIGIN      
        1 cgccgcgatc gcctcgttcg gccgcctcgg ccgcgcgtcc gtggccagcg cggatcgcca
       61 cgccgccggc agcagcacct cgtccggcaa ctgcggcgtg agcggcacca tcacgacgac
      121 cctgaacgac aacgacaaca gcagctcggt cagcgtcggc gactccgcca cgatcgtcta
      181 caacgcctgc catgacagcg cgacgtcgtc ggtcagcggc accatctcgg tgacgatcac
      241 gagcgtctcg ggcaacagct tctcggcgac tgccaacgtg cagaacgtcc aggcgaccga
      301 ccagggcctg agctccacgg tcaacggcca gttcacgctc tcggacgacg agaccgacac
      361 ccaggacgac gagacgctgg cgatcggctc gggcgggttg acggtgtccg tgcagtgcac
      421 cagctacacc gacacgctca ccttcgcgag cggcatgcat atcgagacgg tgtacgacag
      481 cgtcagcggc gacacccgcc tgagcctgaa cggcggcctg agctccagca agctggcggg
      541 cagcctgacg ctgaccacgg tgcagccggt cgtgcagcac gcgaccgatg cctacccgag
      601 ctcgggccag gtgcgcgcgg tcggcgccaa cggctccgcg ctgctgatga cggtgctgaa
      661 cacgacccag gtgcagctgc agctcgacgc cgacggcaat ggctcctacg aggccagctc
      721 gaccgccaac tggagcacgc tgatcggcca gcagggctga cgcccacggg ggcgctacgg
      781 cgccttcccc gcgtgccgcg ccggccgtcg cttccgcgat cgccggcgcg cgcgctttcg
      841 ggcgccgtgt ccggcgatcg ttcgtgacgg acgaccgccg cggcccgtcg cattcggcgc
      901 ggcgcgggcg ccagcttcta gaatccgccg cttcgcccgc aagccgtccc gccgtgaaca
      961 tcgtcgtcaa cgaagacctc aagcattaca tcgacccgct gacgcccgag gagcacgagg
     1021 cgctcgagcg aagcctgctc gccgagggct gccgcgacgc gctcgtgctg tggggcgacg
     1081 tgctggtgga cggccacaac cgccacggca tctgcatgaa gcacggcatc ccgttccaga
     1141 ccgtgcagaa cacccgcttc cgctcgatcg aggacgtgca cctctggatg atcgaccagc
     1201 acctgggacg acgcagcgtg tccgacttcc agcgtggcgt gctcgcgctg cgcaagaagg
     1261 agatcctcga ggcgcgccag gccgcggcgc gcgacgccga gcgcgtcgcc gaggtgcgcg
     1321 gcgaggctcg cccggtggcg gacgatcccg acgcgttgat cggcggcgtg ccgatcgcgg
     1381 cggccgagcc gctgaagacg cgcgaagcgg tcgcccgcgc ggccaagctg agctcggcgc
     1441 aggtcgggat gatcgagaag atccagaagc aggccgcgcc cgaggtggtg gcggcgctga
     1501 agtcgggcac gatctccatc agcgcggcgg ccgccgtcgc cagcctgccg gtggacgagc
     1561 aggtcgcggc cgcgagtgcc g
//
LOCUS       DASVLN010000135         5899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_490376, whole genome shotgun sequence.
ACCESSION   DASVLN010000135 DASVLN010000000
VERSION     DASVLN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490376"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..835
                     /locus_tag="VF453_02950"
     CDS             26..835
                     /locus_tag="VF453_02950"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX7686633.1"
                     /translation="MWAVPSPEPARRSELAPLEFRHPQAEREVRLGEHRVGYALRRGR
                     RRSIGFVVSPEGLAVNAPRWVSLQNIDAAVQAKAGWILRKLHEQRERARRVEAARIEW
                     RDGTTLPFLGETVILVLDPRVAGAVLNADAQALPGVPRLTLHVGLPQAAAPEQIRDVV
                     QSWLQRQALRIFTERCRHFAGELGVTMRRLSLSAAQTRWGSASADGSIRLNWRLVHFG
                     LEVIDYVVAHELAHLREMNHSAAFWEVVRSVVPDFERMRTTLKDVPVPVLD"
     gene            complement(1048..2109)
                     /locus_tag="VF453_02955"
     CDS             complement(1048..2109)
                     /locus_tag="VF453_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7686634.1"
                     /translation="MANADERKLHYPLGDTLPAPGDAIDVAPGVRWLRMPLPFALDHI
                     NLWLLRDRDEAGREGWAIVDCGVANDATRAAWEQVFAHHLDGLPVLRVIVTHMHPDHI
                     GNAAWLCERWDCRLWISATDWNAARLASQSTTGFGGEHAAAFFRRHGLLDPEALAKVR
                     ARSGYYASMVPEVPRQFRRLLDGRVVRIGDHAWRCIAGYGHSPEHMSLHCEALGVLVS
                     GDMVLPRISTNVSVIDVEPEADPLPLYLESIARLRALPAQTLILPSHGRPFTGLHERI
                     GQLERHHDERFADTLAACRAKACSAADLLPVLFRRPLDLHQTTFAMGEAIAHLHALAG
                     RGELRARLDDDGVRRFVTA"
     gene            2212..2688
                     /locus_tag="VF453_02960"
     CDS             2212..2688
                     /locus_tag="VF453_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7686635.1"
                     /translation="MSVSPSPAPKSRAARPAAKASAKSFTITELAAEFDVTPRAIRFY
                     EDVGLLEPGRAGRNRVYSQRERTRLMLTLRGKRLGLSLQEIKQLVDMYDSPSDTVPQL
                     QAFLEVLRSHRRQLELQMEDIRVTLEEIAGHEERCERLLRDATEAAVPLPRARARA"
     gene            2927..4108
                     /locus_tag="VF453_02965"
     CDS             2927..4108
                     /locus_tag="VF453_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX7686636.1"
                     /translation="MSHLPGLNFMLGDDIDALRDAVHAFAQAEIAPRAAEIDRSDQFP
                     MDLWRKMGELGVLGITVPEEYGGADMGYLAHMIAMEEISRASASVGLSYGAHSNLCVN
                     QIKRNGTEAQKKKYLPKLISGEHVGALAMSEPGAGSDVISMKLKAEDKGGVYVLNGSK
                     MWITNGPDADTLVVYAKTEPELGARGVTAFLIEKGMKGFSIAQKLDKLGMRGSHTGEL
                     VFQDVEVPAENILGQLNGGAKVLMSGLDYERAVLAAGPIGIMQAVMDNVVPYIHDRKQ
                     FGQSIGEFQLIQGKVADMYTVLQAARSFCYTVGKNLDALGAGHVRQVRKDCASVILWC
                     AEKATWMAGEGIQIYGGNGYINDYPLGRLWRDAKLYEIGAGTSEIRRMLIGRELFAET
                     M"
     gene            4193..4849
                     /gene="can"
                     /locus_tag="VF453_02970"
     CDS             4193..4849
                     /gene="can"
                     /locus_tag="VF453_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX7686637.1"
                     /translation="MPLTIQDLFSHNRAWAAEMERARPGFFSSLEKQQTPKFMWIGCS
                     DSRVPANQITGLEPGEVFVHRNVANVIVHSDLNALSAVQFAVDMLKVEDIMVVGHYRC
                     GGVLAALQGARIGVADNWIRHIQDVRDRHRALLEQLPADHRADALVDLNAIEQVVNLS
                     VSTVMVDAWAKGQTVRVHGWTFGVHDGLLQDLGVTIDSTDAIEPVYRKAVAAVAAKYA
                     "
     gene            4859..>5899
                     /gene="aceK"
                     /locus_tag="VF453_02975"
     CDS             4859..>5899
                     /gene="aceK"
                     /locus_tag="VF453_02975"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEX7686638.1"
                     /translation="MFPQRLDSPLAYDIARAMLDGFNRHYRLFRAESQRAKHRFETAD
                     WHAQQRAQRERIEFYDLRVREAVQRLEKEFKAGEQPMEVWHQAKLHYIGLLIDHHQPE
                     LAETFFNSVTTKILHRTYFHNDFIFVRPAVSTEYIENDQPAATATYRSYYPTRETLHE
                     NVVRIVDNFQLRCPFDDLERDAGLVVEAIVARMHQVKLRANFQLQVLSSLFFRNKGAY
                     VVGKIINGFSELPFALPLLHDRGKLVIDACLFGEDELQMLFSFARAYFMVDMEVPSAF
                     VQFLRSLMPRKPRAEIYNALGLAKQGKTLFYRDFLYHLKHSSDRFRIAPGIKGMVMLV
                     FDLPSFPYVFKVI"
BASE COUNT          937 a         2073 c         2041 g          848 t
ORIGIN      
        1 ggacgacgcc ccggtgccgg ggcgcatgtg ggccgtgccg tcccccgagc ccgcccgccg
       61 cagcgagctc gcgccgctgg agttccgcca tccgcaggcc gagcgcgagg tgcgcctcgg
      121 cgaacaccgc gtggggtatg cgctgcggcg cggccggcgg cgctcgatcg gcttcgtcgt
      181 gagccccgag ggcctggctg tcaacgcgcc gcgctgggtc tcgctgcaga acatcgacgc
      241 ggcggtgcag gccaaggcgg ggtggatcct gcgcaagctg cacgagcagc gcgagcgcgc
      301 ccgccgcgtc gaggccgcgc gcatcgagtg gcgcgacggc acgacgctgc ccttcctcgg
      361 cgagacggtg atcctcgtgc tggacccgcg cgtcgccggc gcggtgctca acgccgacgc
      421 ccaggcgctg cccggcgtgc cgcggctcac gctgcacgtg ggactgccgc aggcggcggc
      481 gccggagcag atccgcgacg tcgtccagag ctggctgcag cgccaggccc tgcgcatctt
      541 caccgagcgc tgccgccact tcgcgggcga gctgggcgtg acgatgcgcc gcctgtcgct
      601 cagcgcggcg cagacgcgat ggggctcggc gtcggccgat ggctccatcc gcctgaactg
      661 gcggctggtg cacttcgggc tcgaggtgat cgactacgtc gtcgcccacg agctcgcgca
      721 cctgcgcgag atgaaccaca gcgccgcgtt ctgggaagtc gtgcgctcgg tcgtccccga
      781 cttcgaacgc atgcgcacga cgctcaagga cgtacccgtc cccgtattgg actgagcacc
      841 gcgcgcgacg gcgccgcagc ttcgcgagat ttccccccaa aagggggatg cgacctgccg
      901 ggcgggcagg cacagtaacg cctgcatgct gtcgtcaccc cctcagggac acgcatcagg
      961 gagcgaaagc cgggcaagaa ccggcagcag ggccggtctc gattcgacga tcggccttgt
     1021 tttttgtgcg cacggcggcg cgcgtcatca agcggtcacg aagcgccgca cgccgtcgtc
     1081 gtccaggcgc gcgcgcagct cgccgcggcc cgcgagggcg tgcaggtggg caatcgcctc
     1141 gcccatcgcg aaggtggtct ggtgcaggtc cagcgggcgc cggaacagca ccggcagcag
     1201 gtcggcggcg ctgcacgcct tggcgcggca ggctgcgagc gtgtcggcga agcgttcgtc
     1261 gtgatgccgc tccagctgcc cgatgcgctc gtgcaggccc gtgaacgggc gcccgtgcga
     1321 cggcaggatc agcgtctgcg cgggcagcgc gcgcaggcgc gcgatggact cgaggtacag
     1381 cggcagcggg tcggcctcgg gctcgacgtc gatgacgctc acgttggtgg agatccgcgg
     1441 cagcaccatg tcgccggaga ccagcacgcc cagcgcctcg cagtgcagcg acatgtgctc
     1501 gggcgagtgg ccgtagccgg cgatgcagcg ccaggcgtgg tcgccgatgc gcacgacgcg
     1561 gccgtccagc aggcggcgga actggcgcgg cacttccggc accatcgacg cgtagtagcc
     1621 cgagcgcgcc cgcaccttgg cgagcgcctc gggatcgagc agcccgtgcc ggcggaagaa
     1681 cgcggccgcg tgctcgcccc cgaagcccgt cgtcgactgg ctcgccaggc gcgcggcgtt
     1741 ccagtcggtg gcgctgatcc agagccggca gtcccagcgc tcgcacagcc aggccgcgtt
     1801 gccgatgtgg tccgggtgca tgtgcgtgac gatcacgcgc agcaccggca ggccgtcgag
     1861 atggtgcgcg aagacctgct cccaggccgc gcgcgtcgcg tcgttggcga cgccgcagtc
     1921 gacgatcgcc cagccctcgc ggccggcctc gtcgcggtcg cgcagcagcc agaggttgat
     1981 gtgatcgagc gcgaagggca gcggcatgcg cagccagcgc acgccggggg cgacgtcgat
     2041 cgcgtcgccg ggcgcgggca gggtgtcgcc cagggggtag tgcagcttgc gttcgtcggc
     2101 attggccatc gtgatttcct gtggggcgcg ggcgcggcgg cgccgaaaat cggctcgctt
     2161 ttggcgccgg ctggccctag acttccgttt acgttaacgt caagtctacc catgagcgtt
     2221 tccccgagcc cggcgccgaa gtcccgggcc gcccggccgg ccgccaaggc ctccgccaag
     2281 agcttcacga tcaccgagct cgctgccgag ttcgacgtca ccccgcgcgc catccgcttc
     2341 tacgaggacg tcgggctgct cgagccgggg cgcgcgggcc gcaaccgggt ctactcgcag
     2401 cgcgaacgca cgcgcctgat gctgaccctg cgcggcaagc gcctcggcct gagcctgcag
     2461 gagatcaagc agctcgtgga catgtacgac tcgccgtcgg acaccgtgcc gcagctgcag
     2521 gccttcctcg aggtgctgcg cagccaccgc cggcagctcg agctccagat ggaggacatc
     2581 cgcgtgacgc tcgaggagat cgccggccac gaggagcgct gcgagcggct gctccgggac
     2641 gcgaccgagg ccgccgtgcc gctcccgcgc gcccgcgcgc gcgcctgacg cgcgaggcgc
     2701 gcccggcacg gaccgggcgc tcctgattta cgtttacgta aacgtcaatt aaactgcggg
     2761 atccggcgac ccgagccggt ccagagatcc cgtccgcgcc tcggccgtcc tcccgaaggt
     2821 cgacaatgcc tgccggccac ccgcgccggc ggcgagcgcg gatcccccat ccggcggcgc
     2881 gcgccctcgt ggcgccagcg gcccgccagc ccaggagacg agacccatga gccacctgcc
     2941 cggcctgaac ttcatgctcg gcgacgacat cgacgcgctg cgcgacgccg tccacgcgtt
     3001 cgcccaggcc gagatcgcgc cgcgcgccgc cgagatcgac cgcagcgacc agttcccgat
     3061 ggacctctgg cgcaagatgg gcgagctggg cgtcctgggc atcaccgtcc ccgaggaata
     3121 cggcggcgcc gacatgggct atctcgcgca catgatcgcg atggaggaga tcagccgcgc
     3181 cagcgcctcg gtgggcctga gctacggcgc gcactccaac ctgtgcgtca accagatcaa
     3241 gcgcaacggc accgaggcgc agaagaagaa gtacctgccg aagctgatct cgggcgagca
     3301 cgtgggcgcg ctggcgatga gcgagccggg cgccggctcg gacgtcatct cgatgaagct
     3361 caaggccgag gacaagggcg gcgtctacgt gctcaacggc agcaagatgt ggatcaccaa
     3421 cggccccgac gccgacacgc tggtggtcta cgccaagacc gagcccgagc tgggcgcgcg
     3481 aggcgtcacg gcgttcctga tcgagaaggg catgaagggc ttctccatcg cccagaagct
     3541 cgacaagctc ggcatgcgcg gcagccacac cggcgagctg gtgttccagg acgtcgaggt
     3601 gcccgccgag aacatcctcg gccagctcaa cggcggtgcg aaggtgctga tgtccggcct
     3661 cgactacgag cgggcggtgc tcgccgccgg cccgatcggc atcatgcagg cggtgatgga
     3721 caacgtcgtg ccctacatcc acgaccgcaa gcagttcggc cagtcgatcg gcgagttcca
     3781 gctgatccag ggcaaggtcg ccgacatgta cacggtgctg caggcggcgc gttcgttctg
     3841 ctacacggtc ggcaagaacc tcgacgcgct cggtgccggc cacgtgcgcc aggtgcgcaa
     3901 ggactgcgcg tcggtcatcc tgtggtgcgc cgagaaggcg acctggatgg cgggcgaggg
     3961 catccagatc tacggtggca acggctacat caacgactac ccgctcggcc gcctgtggcg
     4021 cgacgccaag ctgtacgaga tcggcgccgg caccagcgag atccgccgta tgctgatcgg
     4081 gcgggagctg ttcgccgaga cgatgtaggg gttcagcgtt gagcgttcag cgttcagcgg
     4141 gaaacccggc acgctcaacg ctcaacgcta accgcggaag cacaatacgc ccatgcccct
     4201 caccatccaa gacctcttct cccacaaccg cgcctgggcc gcggagatgg agcgcgcccg
     4261 cccgggcttc ttcagctccc tggagaagca gcagacgccc aagttcatgt ggatcggctg
     4321 ctcggacagc cgcgtgccgg ccaaccagat cacggggctc gagccgggcg aggtgttcgt
     4381 ccaccgcaac gtggccaacg tgatcgtgca ctccgacctc aacgcgctgt ccgcggtgca
     4441 gttcgcggtg gacatgctga aggtcgagga catcatggtg gtgggccact accgctgcgg
     4501 tggcgtgctc gcggcgctgc agggcgcgcg catcggcgtg gcggacaact ggatccgcca
     4561 catccaggac gtgcgcgacc gccatcgcgc cctgctcgaa cagctgccgg cggaccaccg
     4621 tgccgacgcg ctggtggacc tcaacgcgat cgagcaggtg gtgaacctgt cggtgagcac
     4681 cgtgatggtc gacgcctggg ccaaggggca gacggtgcgc gtgcatggct ggaccttcgg
     4741 cgtgcacgac ggcctgttgc aggacctggg cgtcaccatc gacagcaccg acgcgatcga
     4801 acccgtgtac cgcaaggcgg tggcggcggt ggccgccaag tacgcctagc gggcggccat
     4861 gttcccgcag cgcctcgact cgcccctggc ctacgacatc gcccgggcga tgctcgacgg
     4921 cttcaaccgg cactaccggt tgttccgggc cgagtcgcag cgcgccaagc accgcttcga
     4981 gacggccgac tggcacgcgc agcagcgcgc ccagcgcgaa cgcatcgagt tctacgacct
     5041 gcgggtgcgc gaggcggtgc agcgcctcga gaaggaattc aaggccggcg agcagccgat
     5101 ggaggtgtgg caccaggcca agctgcacta catcggcctg ctgatcgacc accaccagcc
     5161 ggagctggcc gagaccttct tcaactcggt caccaccaag atcctgcacc ggacctactt
     5221 ccacaacgac ttcatcttcg tgcggccggc ggtcagcacc gaatacatcg agaacgacca
     5281 gccggcggcg acggccacct accggtcgta ctaccccacg cgcgagacgc tgcacgagaa
     5341 cgtcgtgcgc atcgtcgaca acttccagct gcgctgcccg ttcgacgacc tcgagcgcga
     5401 cgctggcctg gtggtggaag cgatcgtcgc gcgcatgcac caggtgaagc tgcgcgccaa
     5461 cttccagctc caggtgctgt cgtcgctgtt cttccgcaac aagggcgcgt acgtggtggg
     5521 caagatcatc aacggctttt ccgagctgcc gttcgcgctg cctttgctgc atgaccgcgg
     5581 caagctcgtc atcgacgcgt gcctgttcgg cgaggacgag ctgcagatgc tgttctcctt
     5641 cgcgcgggcc tacttcatgg tggacatgga ggtcccgtcg gccttcgtgc agttcctgcg
     5701 ctcgctgatg ccgcgcaagc cgcgcgccga gatctacaac gccctgggcc tggccaagca
     5761 ggggaagacg ctgttctacc gcgacttcct ctaccacctg aagcactcca gcgaccgctt
     5821 ccgcatcgcc cccggcatca agggcatggt gatgctggtg ttcgacctgc cgtcctttcc
     5881 ctacgtgttc aaggtcatc
//
LOCUS       DASVLN010000136         1753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_382743, whole genome shotgun sequence.
ACCESSION   DASVLN010000136 DASVLN010000000
VERSION     DASVLN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382743"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VF453_02980"
     CDS             <1..351
                     /locus_tag="VF453_02980"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX7686639.1"
                     /translation="LRAAVAAAPFMVAGTGRLDTVLMQRLGARLFCKVGAEGVYCASL
                     PELGLGLALKMDDGNTARAAEVALVALVEALLPLADDDRAFLAPLRDVTMRNWNGRVV
                     GRLAAVPQVDARSR"
     gene            complement(360..1136)
                     /locus_tag="VF453_02985"
     CDS             complement(360..1136)
                     /locus_tag="VF453_02985"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7686640.1"
                     /translation="MNVLLVYAHPEPRSLNGALRDFTLRRLEAAGHAVEVSDLYAMKW
                     KAVVDAADSLDPPAGARFDPAGDSRRAYEHGRQSPDIAAEQDKLRRADVLILQFPLWW
                     FSMPAILKGWVDRVYAYGFGYGVGEHSDRRWGDRYGQGTLAGKRAMLVVTAGGWESHY
                     GPRGINGPIDDLLFPIQHGILYYPGFDVLPPFLAFRTGRMDAERFARTCEALGERLDA
                     LATTPPIAFRPQNGGDYEIPSLTLRDEHAPGRGGFGVHTA"
     gene            1227..>1753
                     /locus_tag="VF453_02990"
     CDS             1227..>1753
                     /locus_tag="VF453_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7686641.1"
                     /translation="MINLARLDLNLLVTLDALLAERHVTRAARRLHLSQPAVSAQLAR
                     LRDALGDPLLLPGPRGMRPTARAESLREPLRMALEALEQAVAPASPFDPAQAGNTWRV
                     AATDYGESTIALPALNTLRTSAPGTRLAMLELVPTRIEKQAEQGEIDLAFHTTEGAPP
                     GLRRRALFTERYVLIG"
BASE COUNT          251 a          629 c          632 g          241 t
ORIGIN      
        1 ctgcgcgcgg cggtcgcggc cgcgccgttc atggtcgccg gcacggggcg gctcgacacc
       61 gtgctgatgc agcgcctggg cgcgcgcctg ttctgcaagg tcggcgccga aggcgtgtac
      121 tgcgcgagcc tgccggagct cggcctgggc ctcgcgttga agatggacga cggcaacacc
      181 gcccgcgcgg ccgaggtcgc gctggtcgcc ctcgtcgagg cgctgctgcc gctcgccgac
      241 gacgaccgcg ccttcctcgc gccgctgcgc gacgtgacga tgcgcaactg gaacggccgc
      301 gtggtcggcc gcctggcggc ggtgccgcag gtcgacgcgc gatcgcgctg acggcggcgt
      361 caggccgtgt gcacgccgaa gccgccgcgg ccgggcgcgt gctcgtcgcg cagcgtgagc
      421 gacgggatct cgtagtcgcc gccgttctgc ggccggaagg cgatcggcgg cgtcgtcgcg
      481 agggcgtcca ggcgctcgcc cagcgcctcg caggtgcggg cgaagcgctc ggcgtccatg
      541 cggcccgtcc ggaaggcgag gaacggcggc agcacgtcga agccggggta gtacaggatc
      601 ccgtgctgga tgggaaacag caggtcgtcg atcgggccat tgatgccgcg cgggccgtag
      661 tgcgactccc agccgcccgc cgtgacgacc agcatcgcgc gcttgccggc cagcgtgccc
      721 tggccgtagc ggtcgcccca gcggcgatcg gagtgctctc cgacgccata cccgaacccg
      781 tacgcgtaca cgcgatcgac ccagcccttg aggatcgccg gcatcgagaa ccaccacagc
      841 gggaactgca ggatcagcac gtcggcgcga cgcagcttgt cctgctcggc ggcgatgtcg
      901 ggactctggc ggccgtgctc gtaggcgcgc cgcgagtccc cggcgggatc gaaacgcgcg
      961 ccggcgggcg gatccaggct gtcggcggcg tcgaccaccg ccttccactt catggcgtag
     1021 aggtcggaca cctcgactgc gtggcccgcg gcctcgaggc gacggagggt gaagtcacgc
     1081 agcgcgccgt tcagggagcg cggctcggga tgggcgtaga cgaggaggac gttcatggcg
     1141 cggcggtcga ggtcgttgcg atggcgccca tcttcggcac gcgttcgata tattggaaat
     1201 cgatttccac gatttccggt atccacatga tcaatctcgc tcggctcgac ctcaacctgc
     1261 tcgtcacgct ggacgcgctg ctggccgaac gccacgtcac ccgcgcggcg cggcggctgc
     1321 acctgtcgca gccggcggtg agcgcccagc tcgcgcgcct gcgcgacgcg ctcggcgacc
     1381 cgctgctgct gccgggcccg cgcggcatgc gcccaaccgc ccgcgctgaa agcttgcgcg
     1441 aaccgctgcg gatggcgctc gaagcgctgg agcaggccgt cgcgcccgcc agcccgttcg
     1501 acccggccca agccggcaac acctggcgcg tggccgccac ggattacggc gaatccacca
     1561 tcgcattgcc ggcgctgaac accctgcgca caagcgcgcc gggcacgcgg ctcgccatgc
     1621 tggagctggt gcccacgcgt atcgaaaagc aagccgagca aggagagatc gatctcgcgt
     1681 tccacaccac agagggcgca ccacccggcc tgcgccgccg cgcgctattc actgagcgct
     1741 atgtgctgat cgg
//
LOCUS       DASVLN010000137         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_382913, whole genome shotgun sequence.
ACCESSION   DASVLN010000137 DASVLN010000000
VERSION     DASVLN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382913"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1424)
                     /locus_tag="VF453_02995"
     CDS             complement(48..1424)
                     /locus_tag="VF453_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX7686642.1"
                     /translation="MRLKSKLLAVAAIPLLLSLALIAAAVLLQERELARREHALVEGG
                     YVSARRAELQHYTELALSAIRPLVDSGQDDAATRAAAMRLLAAMNYGDDGYFFVYDLH
                     GKVLMHPRQPELVGRDLWDMRDPRGRMTIQELIARAHAGGGYVDYLWRRPSTGQLAPK
                     LGYVVEIPQWGWMMGTGLYRDDIDAATAQLDAQASSNIGATLLWIAAIACGGVALISV
                     SGVWLNLSEGRVADAKLQLLARQVVRFQEDERAHLARELHDGATQTMVSAKLHVESAV
                     DQLEREHRAAPPALARALARLKESLDEVRRISHRLRPAMLDELGLPAALELLGREWDE
                     TQAHAMRVEILGEPVGLPEEVKTALFRVAQEALANIAKHAQAREVSIALHFAEGGVAL
                     RIVDDGRGFEVDTVQLDPRRGIGLRNMRERLASIGGRFEIQASPGQGTVLDAVVPAAA
                     LRRLRVAG"
BASE COUNT          194 a          564 c          564 g          196 t
ORIGIN      
        1 tgagcgcggc cttcctcgac gcgctggagg cggcgcgcgg cggctgatca gcccgccacg
       61 cgcagccgcc gcagcgccgc ggcgggcacg acggcgtcga gcaccgtgcc ctgccccggc
      121 gaggcctgga tctcgaagcg ccctccgatc gacgcgaggc gctcgcgcat gttgcgcagg
      181 ccgatgccgc gccgagggtc gagctgcacc gtgtcgacct cgaagccgcg gccgtcgtcg
      241 acgatgcgca gcgccacgcc cccttccgcg aagtgcagcg ctatcgacac ctcgcgcgcc
      301 tgcgcgtgct tggcgatgtt ggcgagcgcc tcctgcgcga cccggaacag cgcggtcttg
      361 acctcctcgg gcaggccgac gggctcgccc aggatctcga cgcgcatcgc gtgcgcctgc
      421 gtctcgtccc actcgcgccc gagcagctcc agcgccgcgg gcaggcccag ctcgtcgagc
      481 atcgccggac gcaggcggtg cgagatccgc cgcacctcgt cgagcgattc cttcagccgg
      541 gcgagcgcgc gcgccagcgc cggcggcgcg gcgcggtgct cgcgctcgag ctggtcgacc
      601 gccgactcca cgtgcagctt ggccgagacc atggtctggg tcgcgccgtc gtggagctcg
      661 cgcgcgaggt gggcgcgctc gtcctcctgg aagcgcacga cctggcgcgc gagcagctgc
      721 agcttcgcgt cggcgacgcg gccctcgctc aggttgagcc agacgccgct gacgctgatg
      781 agcgcgaccc cgccgcacgc gatcgccgcg atccagagca gcgtcgcgcc gatgttggac
      841 gaggcctggg cgtcgagctg cgcggtggcg gcgtcgatgt cgtcgcgata cagcccggta
      901 cccatcatcc agccccattg cgggatctcg acgacgtagc ccagcttggg cgcgagctgg
      961 ccggtggacg gccggcgcca caggtagtcg acgtagccgc cgcccgcgtg cgcgcgcgcg
     1021 atcagctcct ggatcgtcat gcggccgcgc gggtcgcgca tgtcccagag gtcgcgcccg
     1081 acgagctcgg gctggcgcgg gtgcatcaac accttgccgt gcaggtcgta gacgaagaag
     1141 tagccgtcgt cgccgtagtt catcgccgcg agcaggcgca tcgcggcggc gcgggtcgcg
     1201 gcgtcgtcct gcccggagtc gacgagcggc cggatggcgc tcagcgcgag ctccgtgtag
     1261 tgctgcagct ccgcgcgccg cgcgctcacg tagccgccct cgaccagcgc gtgctcgcgg
     1321 cgggcgagct cgcgctcctg cagcagcacc gccgcggcga tcagcgcgag cgacagcagc
     1381 agcgggatcg ccgcgacggc gagcagcttg gacttcaggc gcatggggac gattctgcag
     1441 cggcacgggc cgcggcggcg cgcgggcgcg ccaagcttcg cacggtggga cgcgcgggcg
     1501 acacggcggc atcccgca
//
LOCUS       DASVLN010000138         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_437205, whole genome shotgun sequence.
ACCESSION   DASVLN010000138 DASVLN010000000
VERSION     DASVLN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437205"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VF453_03000"
     CDS             <1..792
                     /locus_tag="VF453_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX7686643.1"
                     /translation="VTHGISVGAVLPRIWAGPHPVATIANGAKAGKPTVLDRPQVARA
                     FDGLYAGDDAMSRAYRESQASRREVDDAMNSSDAGDMAREQRIADNGAPLPNGFPEDA
                     ARLATLMRRDANVQVAFMALGGWDTHVNQGGASGQLAGRLRPLGQGLAALASGLGPAF
                     DDTTVVVLSEFGRTLRQNGTGGTDHGHGNVMWVLGGEVAGAKVHGRWPGLDASALYEG
                     RDLAVTTDFRQVLAGVCQHQLGLPDARLADVFPGFDATPLDLARA"
     gene            949..1644
                     /locus_tag="VF453_03005"
     CDS             949..1644
                     /locus_tag="VF453_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686644.1"
                     /translation="MDVCTACCCPVEVERELREWPLARLTARHFYQYNDSAKSEVQPA
                     AELRHLLPRLLELIASGEDIHHSTELYLDRLGRCPEGTWSADERAALDRFASAFFDAL
                     LRVGTPGRGLRVWMDTPLSVLLMFDIGGIDIGPLLDLWLRCDDPGSTVQFVESTYWDF
                     WQGCEIRNPFAADRPAFRARVRGWLLEPANRARFVEKMLAPAFLERAREQPARGRIAF
                     ATMVEAVFDQLTA"
     gene            complement(1629..>2206)
                     /locus_tag="VF453_03010"
     CDS             complement(1629..>2206)
                     /locus_tag="VF453_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase"
                     /protein_id="HEX7686645.1"
                     /translation="VAVALDRWWPTAGTHLTGERNQVPRVVRRGGELVRMALPAERST
                     HAFSPAFGDVATIELPFSEIVTLSHHLAVPDIRSWLSASSLDEIRDASTPAPAAVDAR
                     GRSAQRFEMIVQARRGSTLRTASARGQDIYAVSAPLVVEAARRLAAPGADARGALTLG
                     QAFDAGDFLAALVRQGELVIDLPGVAAQAVN"
BASE COUNT          291 a          806 c          809 g          300 t
ORIGIN      
        1 gtcacgcacg gcatcagcgt cggcgcggtg ctgccgcgca tctgggccgg gccgcatccg
       61 gtcgcgacga tcgccaacgg cgccaaggcc ggcaagccga cggtgctcga tcgcccgcag
      121 gtcgcgcgcg cgttcgacgg cctgtacgcc ggcgacgacg cgatgtcgcg cgcctatcgc
      181 gagtcgcagg cctcgcgccg cgaggtcgac gacgcgatga actcgagcga cgccggcgac
      241 atggcgcgcg agcagcgcat cgccgacaac ggcgcccccc tgcccaacgg cttccccgag
      301 gacgccgcgc gcctggccac gctgatgcgg cgcgacgcca acgtgcaggt cgccttcatg
      361 gcgctgggcg gctgggacac gcacgtcaac cagggcggcg cgtccggcca gctcgccgga
      421 cggctgcggc cgctcggcca ggggctcgcg gcgctcgcct ccggactcgg gccggcgttc
      481 gacgacacca cggtggtcgt cctctcggaa ttcggccgca cgctgcgcca gaacggcacc
      541 ggcggcaccg atcacggcca cggcaacgtg atgtgggtgc tgggcggcga ggtcgccggc
      601 gccaaggtgc acggccgctg gcccgggctg gacgcgtccg cgctgtacga gggccgcgac
      661 ctcgccgtca ccaccgactt ccgccaggtg ctcgcgggcg tctgccagca ccagctcggc
      721 ctgcccgacg cgcggctcgc ggacgtgttc ccggggttcg acgcgacgcc gctggatctg
      781 gcgcgggcct gacccggcct tccaggcaat gaccggagcc gtcgaccggg aaccatgatg
      841 cctgccaccg caccggcgac gctggccgac atcccggaca cggcgcttcg cgacgccgtg
      901 gcgcgggccc atgccgtctt cggccactgc ggggcgccgg cgttcccgtt ggacgtctgc
      961 accgcctgct gctgtcccgt cgaggtggag cgcgagctgc gcgaatggcc gctcgcccgg
     1021 ctgactgcac gccacttcta ccagtacaac gattccgcca agagcgaggt acagcccgcc
     1081 gccgagcttc gccacctgct gccgcggctg ctcgagctca tcgcgtccgg agaggacatc
     1141 catcactcca ccgagctgta cctcgatcgt ctgggccgct gccccgaggg cacgtggagc
     1201 gctgacgaac gcgcggcgct ggatcgcttc gcgtcggcct tcttcgatgc gctgcttcgc
     1261 gtcggtacgc ccgggcgcgg gttgcgcgtc tggatggaca ccccgctcag cgtgctgctg
     1321 atgttcgaca tcggcggcat cgacatcggg ccgctgctcg atctctggct gcgctgcgac
     1381 gatccgggct cgacggtgca gttcgtggag tcgacctact gggacttctg gcagggctgc
     1441 gaaatccgca atcccttcgc agccgatcga ccggcgttcc gcgcgcgcgt gcgcgggtgg
     1501 ctgctggagc cggcgaatcg cgcgcggttt gtcgagaaga tgctggcgcc ggcattcctg
     1561 gaacgtgccc gcgagcagcc tgcgcgggga cggatcgcgt tcgcgacgat ggtcgaggcg
     1621 gtgttcgatc aattgacggc ctgagccgcc acgcccggaa ggtcgatgac gagttcgccc
     1681 tgccgaacca gcgccgcgag gaaatcgccc gcgtcgaacg cctgccccag cgtgagcgcc
     1741 ccgcgcgcgt ccgcgccggg cgcggccagt cgccgcgctg cctcgacgac gagcggggcg
     1801 ctcaccgcgt agatgtcctg cccgcgcgcg ctggccgtgc gcagggtcga gccgcggcgg
     1861 gcctggacga tcatctcgaa gcgctgcgcc gaacggccgc gcgcgtcgac ggcggccggg
     1921 gcgggggtgg aggcgtcgcg gatctcgtcc agcgacgacg cgctgagcca ggagcggatg
     1981 tcgggcacag ccaggtggtg cgagagcgtg acgatctcgc tgaacggcag ctcgatcgtc
     2041 gcgacgtcgc cgaacgcggg cgagaaggcg tgggtcgagc gctcggcggg cagcgccatc
     2101 cgcaccagct cgccgccgcg ccgcaccacg cgcggcacct ggttgcgctc gccggtcagg
     2161 tgcgtgcccg cggtcggcca ccagcggtcg agggcgacgg cgacga
//
LOCUS       DASVLN010000139        14800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_114403, whole genome shotgun sequence.
ACCESSION   DASVLN010000139 DASVLN010000000
VERSION     DASVLN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14800
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114403"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /gene="thiL"
                     /locus_tag="VF453_03015"
     CDS             complement(<1..548)
                     /gene="thiL"
                     /locus_tag="VF453_03015"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX7686646.1"
                     /translation="MGEFDLIAKYFSRPVRRAVLGGGDDCALLQPTPGLQWAVSTDAL
                     VEGRHFLSTVPPDALGHKALAVNLSDLAACGAQPVAFTLSLTLPRVDEPFLEGFAQGL
                     YALADAHGCELVGGDTTAGPLNIGVTVFGEVPPGQALRRGGAHAGDELWVSGRLGDAR
                     LALEVFRGHVAPRGEAFDELRRA"
     gene            787..3081
                     /locus_tag="VF453_03020"
     CDS             787..3081
                     /locus_tag="VF453_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX7686647.1"
                     /translation="MTTPEDKRAELRQDALRYHEFPTPGKIAVTPTKQLTNQRDLALA
                     YSPGVAAACEEIVADPTNAFRYTARGNLVAVITNGTAVLGLGNIGPLAAKPVMEGKGV
                     LFKKFAGIDVFDLELHEPDLDKLVDAIAALEPTFGGINLEDIKAPDCFYVERKLRERM
                     KIPVFHDDQHGTAIVVGAGLINALKVSGKRIEDVKLVASGAGAAALACLNLLLKLGLP
                     RRNVWVTDLAGVVYQGRAELMDPDKAEFAQDTPLRTLTEAIEGADVFLGLSAGGVLKP
                     AMVAKMAANPIIFALANPTPEISPEDVQAVRSDAIMATGRSDYPNQINNVLCFPYIFR
                     GALDCGATTINDAMEVAAVHAIAELAQAEQSEVVAAAYAGADLSFGRDYLIPKPFDPR
                     LMMKIAPAVARAAIDSGVALRPIADMDVYRERLQTFVYASGTAMKPIFALAKVAVHRR
                     VAYAEGEEERVLRAVQVVVDENIARPVLIGRPAIIAQRCEKFGLRLQEGRDYEVVNTE
                     YDERFRDYWQTYHHMTERKGVTAQLAKIEMRRRLTLIGAMMLHKGEVAGLVCGTWGST
                     ANHLLYIDQVIGKRAGVNTYACMNGLILPGRQIFLVDTHVNADPSAVQLAEITMMAAE
                     EMKRLGILPKAALLSHSSFGSSSQPSAQKMRETLALLREQAPWLEVDGEMHGDAALDP
                     AYRAKIMPRSTLTGEANLLVMPNIDAANISYNLLKTAAGGGIAIGPVLLGAARPVHVL
                     TPSTTVRRIVNMTALTVADANAAR"
     gene            3642..3986
                     /locus_tag="VF453_03025"
     CDS             3642..3986
                     /locus_tag="VF453_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HEX7686648.1"
                     /translation="MAGAAVPGLSPARSAPGLGDPVTLSSLPREAQRTWQLIHSGGPF
                     PYSKDGVVFGNREKRLEREPRGYYHEYTVPTPGARDRGARRIICGGEHLTEPVACFYT
                     EDHYNSFHRIVK"
     gene            4032..4427
                     /locus_tag="VF453_03030"
     CDS             4032..4427
                     /locus_tag="VF453_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEX7686649.1"
                     /translation="MLLQTVRPNIVQSIRAYRVDELLEAAQGAGQHFLYANLTNAQSK
                     QDVLEQIAEAFLFPAHFGKNLDALYDAMTDLVHKSGSQPGFVVVLEQLPDNPKFDRES
                     REQLLDVFRDAADFWGERKIAFRCFYSFQ"
     gene            4406..4597
                     /locus_tag="VF453_03035"
     CDS             4406..4597
                     /locus_tag="VF453_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686650.1"
                     /translation="MLLFFSVARSQENGSWERAAEAAPIVEKPATKASSKANANANFG
                     MNMPLMSSAFDTAMWLNAA"
     gene            4671..5516
                     /locus_tag="VF453_03040"
     CDS             4671..5516
                     /locus_tag="VF453_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686651.1"
                     /translation="MPDFLRRLLRRAEPPRATSVHPALGAALVRHEAWADDVGTQDAT
                     VAPLQVDGDPAPSRRVADARDPQAPRDVFVAPGGLGLPGVALANMRDPQARVQLWELG
                     TTTPAGGTTFARALELVFPPGRDAAGWQVRQLVPLPRLQCVVVLCRDDDPRTALVAVL
                     DLATRRLRELGVAEPDPFTDGPDGRPVHLAALQAGPDAVLVRWHSGRVRLGQWGDVAQ
                     EDRVVLFTPRERDGLPVLTLALDDGNIRGWGLQGTTLWLHAIDGRPRPAPREYVWSLA
                     LGAVL"
     gene            5902..6771
                     /locus_tag="VF453_03045"
     CDS             5902..6771
                     /locus_tag="VF453_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686652.1"
                     /translation="MLAVGCEPAQSSGSNQEDTMFFGLFKSKSPSTVDPSVGAALVRE
                     AAFPKGETQPPVPAYPQLRRVDLTAAPPEGRISPKAIEEPAADAYVTSGAPGSSALVN
                     TYDPERRCELWTIDAKDPSRLVGQRQLRFEADQSRWIMFGVGDAVALPGNKLLVQLRT
                     FDPGPTDRAYVIDLSAGTARSLGVIRPDWPAGLPFHYLDSLQLRPDAVLAVFRTDQLR
                     LRAEHYVNRFDHLLLFSPRHPDGLEIARIGIDDGNVRGWRLIGSKLWLQTSDDRGDAP
                     KVHVWSLDLTRVL"
     gene            6964..8157
                     /locus_tag="VF453_03050"
     CDS             6964..8157
                     /locus_tag="VF453_03050"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX7686653.1"
                     /translation="MVLGDGETQHTLSKRDVNTSLANTKLRGANLEAVFQGSTAGVKT
                     GFGYVLSFNRNGLKHAVIATRGTRVEHSIADGLADLHAAITTFGGYGQVHAGFYNAFA
                     SIMPNLASQERALMDADVVHCVGHSLGGAIATLIAAHYAGRRGAGVKLYTFGSPRVGA
                     YATPQAFESMIGKDSIYRVAHDLDPVTLVGPFPYSHVNGLPDDQNNMMLRSPTARLIS
                     VANHDMMQYVESIMPGGETGMKWANIRRMSAGVVHDNAVLARWLLRSVDQPSMFTQKV
                     VEGLSVLLKAFAHFLQMQQVTSAIISGMTAIDMFSAALAKGIERVAGTSPEIMASLSI
                     AAAWARVAVVGMKFTAAVIKAIMMRMMAVLKPIASRALTLVGAGDPLPLVLAGATVIA
                     GTMLG"
     gene            8186..8752
                     /locus_tag="VF453_03055"
     CDS             8186..8752
                     /locus_tag="VF453_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686654.1"
                     /translation="MDRQSFEHLYLHANSIATLAFLRAMARIIAMPAPTEPSPAMQEF
                     AAAIEHQMQHPETFDWSVYDGIDEGVDDLIRQREEAEASGLPFFGDCVAMAEEDEAEG
                     EMPDVGRFLERARAAAAALAARIDDSFARVDHAALADALATLPRDLPPLTTALSAEDL
                     ERWIVLYEGEHEGLQRAVEETRKPEPVL"
     gene            complement(8773..9543)
                     /gene="rsmA"
                     /locus_tag="VF453_03060"
     CDS             complement(8773..9543)
                     /gene="rsmA"
                     /locus_tag="VF453_03060"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX7686655.1"
                     /translation="MAHIPRKRFGQHFLTATDVIERIVRAIDPRPGERLVEIGPGLGA
                     MTIPLRARCDALTVVELDRDLAARLRQRGDLDVVESDVLKVDFAALADRLGGPIRVVG
                     NLPYNISSPILFHLIAAAPRVVDQVFMLQKEVVDRMAAGPGGKDFGRLSVMLQWRYEI
                     EHLFDVAPDAFDPPPKVDSAIVAMRPRPAEVAARADPVLLGELVAVAFSQRRKILRNT
                     LGKWLDERGFDGTFDVQRRAEEVPVDEYLALARAVKAA"
     gene            complement(9548..10942)
                     /locus_tag="VF453_03065"
     CDS             complement(9548..10942)
                     /locus_tag="VF453_03065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7686656.1"
                     /translation="MSLVPSPVALMLAVVLAFAAAAPASAQEPAKTVAISAGSAASAP
                     APAPAPKTARRPESVPGDYIVAVVNDESVTNNEVTQRLAKVREEDAEKGGPQPTETEL
                     RKQALDQLIEERVLTTHAREIGVKVDDAEVDRAIDNIAAGNKLTSAQLRDRLRQQGMD
                     WTTFRNNIRDQITIERVREHEVVSHIHVSDSEVADYIAKLKEGHSTAPQVDIAQILVT
                     VPDKASPEEVAERKARAEQALARVNNGEPFEQVAREMSEDTNRAKGGEMGSRPLDRYP
                     DLFAKAIEGVKVGGTTPVIRSGAGFHILKVLSRGEESGITIVQTRVRHIVLRPSAELS
                     LDAAERRMLDLRQLIASGARTFEEVARQYSEDGSAAQGGDLGWISPGSLVPEFEQAMD
                     KLPVGGLSMPVRSRFGVHLIQVLDRREQVIEPKQLREQALNALREQKFEPAYQEWVAD
                     LRAKAFIEYRDPPQ"
     gene            complement(10954..13257)
                     /gene="lptD"
                     /locus_tag="VF453_03070"
     CDS             complement(10954..13257)
                     /gene="lptD"
                     /locus_tag="VF453_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX7686657.1"
                     /translation="MRPTPTPAPAREVAGSDQPPPQITLTADNVVSHIGGVAQASGGV
                     DLKRQDLELKADLLSYDQLSDTAHAEGHVRIARGLDWFSADRLDLELTRYAGTLLDTE
                     YEIGSRKAGGKAQRIELEDRDHSTLYQADYTACPRDLPAGQEPDWIIKGDRIDIDMAT
                     NQGRAEHAVLRFLGVPILAAPVLTFPVTADRKSGWLPPTAEASNRSGPGLGVPYYWNL
                     RPNVDATFTPAYITRRGPTLTSELRYLEPGDLGQTTLYLLPDDKIEDKTRGSLEWAHE
                     GVRGDWLSYSARVQRVSDDTYWKDFPGQLSAVTQRLLPTDLSGVRRFMPWGPAGEIDA
                     YARIQRFQTLQDLEDPAAVIQVPYQRSPQLGVRGSLLLDDRTRFEFETEGNRFDLSDK
                     VSTGDLAAAGLPPDTREGGTRVHLIGTVSRAFVSDWGRVEPRLSINGATYHTDSPMSD
                     GRTTATRWIPTFSTDSSASFERQTSLFGRDLVQTLEPRLLYVYTPYHDQTALPLYDTA
                     PKDFNEVSIYSDNQFTGIDRISDENQLTAGATTRFNDAVNGRELLRLGVAQRLLFKDQ
                     ALTADGTPDTRKLSDLLIWGSSSAMEHWSYDGTVEINPSSSWTQRAIVSARYHPGPFR
                     SLSLTYRFARGSSESYEVAGQWPLYHNAPGTGRSGGSRCGDGGTLYGVGRIDYSVSDR
                     RSTYAVGGVEYDANCWIARFVVERTSTGVNESTNHYMLQLELNGLSALGFGSPTKALK
                     NNVPGYQPLRDDAGLFPSSTASSTTSQ"
     gene            13549..14637
                     /locus_tag="VF453_03075"
     CDS             13549..14637
                     /locus_tag="VF453_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7686658.1"
                     /translation="MNDLSAGHAAAQRLPVTWADAEREQAFGRWLHAIAAKHRLDPSS
                     LRLASADASARRYLRIDAVASDDAAVHSYIVMDSPAAEDSIAAFVHVGGLIRAADLNG
                     PRVLEEDQRQGFLLLDDLGDTPYLAALQDADAGAADALMRDAIAALVQWQLRVDPATL
                     PPYDEALLRRELQLFPDWCVAREFGIAWGEAEQKLWADVSRRLIDSALAQPQVAVHRD
                     WMPRNLMVCERNPGVLDFQDAVRGPISYDIASLLRDAFISWDEERELDWAVRYWQRAR
                     AAGLPVDPDFGEFWRATEWMGLQRHLKILGIFCRLKHRDGKPRYSEDLPRFFGYATRV
                     AHRYAPLRGLLKLLEPMSGQAVAAGFTF"
BASE COUNT         2232 a         5219 c         5167 g         2182 t
ORIGIN      
        1 gcgcggcgca gctcgtcgaa ggcctcgccg cgcggcgcga cgtgtccgcg gaacacttcc
       61 agcgcgaggc gcgcgtcgcc gagccggccg ctgacccaga gctcgtcgcc ggcgtgcgcc
      121 ccgccgcgcc gcaatgcctg ccccgggggc acttcgccga acaccgtgac gccgatgttg
      181 agcgggcccg cggtggtgtc gccgcccacg agctcgcagc catgcgcgtc cgcgagcgcg
      241 tacaggcctt gcgcgaagcc ttcgaggaag ggctcgtcga cgcgcggcag cgtcagcgag
      301 agcgtgaatg ccacgggctg cgcgccgcag gccgcgaggt cggacaggtt caccgcgagc
      361 gccttgtggc ccagggcgtc gggcggcacg gtgctcagga agtggcggcc ctcgaccagc
      421 gcgtcggtcg agacggccca ctgcaggccg ggcgtcggct gcagcagggc gcagtcgtcc
      481 ccgccgccca gcacggcgcg gcggacggga cgggagaagt acttcgcgat gaggtcgaat
      541 tcgccgatgg gcatggcggc gcattgtcgc ccagcgcgcg tccggactcg acggatgtgg
      601 agacccgtca ttgacggccg cggcgccgcg gctgcaaagt attcggctgg cgaatgaccc
      661 catgccgatc gctcgcgcac cggcggccgg gcacgccggc gggaaacccc cgctcctaca
      721 attcgccgcc ccgaagccgc gcgccgtgga cgcggcgcgg ccccgaggac gacaccagga
      781 ctgccgatga ccactcccga agacaagcgc gccgagctgc gccaggacgc gctgcggtat
      841 cacgagttcc ccacccccgg caagatcgcc gtcaccccca ccaagcagct gaccaaccag
      901 cgcgacctag cgctggccta ctcgccgggc gtggccgccg cctgcgagga gatcgtcgcc
      961 gacccgacca acgcgttccg ctacacggcg cgcggcaacc tcgtggcggt catcaccaac
     1021 ggcaccgcgg tgctggggct gggcaacatc gggccgctgg ccgccaagcc ggtgatggag
     1081 ggcaagggcg tcctgttcaa gaagttcgcg ggcatcgacg tgttcgacct cgagctgcac
     1141 gagcccgacc tcgacaagct cgtcgatgcc atcgccgcgc tcgagcccac cttcggcggc
     1201 atcaacctcg aggacatcaa ggcgccggac tgcttctacg tcgagcgcaa gctgcgcgag
     1261 cgcatgaaga tccccgtctt ccacgacgac cagcacggca cggccatcgt ggtcggcgcg
     1321 gggttgatca acgcgctcaa ggtcagcggc aagcgcatcg aggacgtcaa gctcgtcgcc
     1381 tcgggcgcgg gcgccgcggc gctcgcatgc ctgaacctgc tgctcaagct gggcctgccg
     1441 cgcaggaacg tgtgggtgac cgacctggcg ggcgtcgtct accagggccg cgccgagctg
     1501 atggaccccg acaaggccga gttcgcgcag gacacgccgc tgcgcacgct gaccgaggcg
     1561 atcgagggcg cggacgtgtt cctgggcctg tcggcgggcg gcgtgctcaa gccggccatg
     1621 gtcgcgaaga tggccgccaa tccgatcatc ttcgcgctcg ccaacccgac gccggagatc
     1681 tcgcccgagg acgtccaggc cgtgcgctcg gacgcgatca tggccacggg ccgctcggac
     1741 tacccgaacc agatcaacaa cgtcctctgc ttcccgtaca tcttccgcgg cgcgctggac
     1801 tgcggcgcga cgacgatcaa cgacgcgatg gaggtcgcgg ccgtgcacgc gatcgcggag
     1861 ctcgcgcagg ccgagcagag cgaggtcgtg gccgccgcgt acgccggcgc cgacctcagc
     1921 ttcggccgcg actacctgat ccccaagccc ttcgacccgc gcctgatgat gaagatcgcc
     1981 cccgcggtcg cgcgcgcggc gatcgactcg ggcgtcgcgc tgcgcccgat cgcggacatg
     2041 gacgtctacc gcgagaggct gcagaccttc gtctacgcct cgggcacggc gatgaagccg
     2101 atcttcgcgc tcgccaaggt cgcggtgcac cgccgcgtcg cctacgccga gggcgaggag
     2161 gagcgcgtgc tgcgcgcggt gcaggtggtg gtcgacgaga acatcgcccg gccggtgctg
     2221 atcggccgcc cggccatcat cgcccagcgc tgcgagaagt tcggcctgcg cctgcaggaa
     2281 ggacgcgact acgaggtcgt caacaccgag tacgacgagc gcttccgcga ctattggcag
     2341 acctaccacc acatgaccga gcgcaagggc gtgacggccc agctcgcgaa gatcgagatg
     2401 cggcgccgcc tgacgctgat cggggcgatg atgctgcaca agggcgaggt cgccggcctg
     2461 gtctgcggca cctggggctc gacggccaac cacctgctct acatcgacca ggtcatcggc
     2521 aagcgcgccg gcgtcaacac ctacgcgtgc atgaacgggt tgatcctgcc cggacgccag
     2581 atcttcctgg tcgacaccca cgtcaacgcc gacccgagcg cggtgcagct ggccgagatc
     2641 acgatgatgg ccgccgagga gatgaagcgc ctgggcatcc tgccgaaggc ggcgctgctg
     2701 tcgcactcca gcttcggctc gagcagccag ccctcggcgc agaagatgcg cgagacgctg
     2761 gccctcctgc gcgagcaggc gccgtggctg gaggtcgacg gcgagatgca cggcgacgcg
     2821 gcgctcgacc cggcctaccg ggccaagatc atgccgcgct ccacgctcac gggcgaggcc
     2881 aacctgctcg tgatgcccaa catcgacgcg gccaacatct cgtacaacct gctcaagacc
     2941 gcggccgggg gcggcatcgc catcggcccg gtgctgctcg gcgccgcgcg gccggtgcac
     3001 gtgctcacgc cgtcgacgac ggtgcgccgc atcgtcaaca tgacggcgct gaccgtcgcg
     3061 gacgccaacg ccgcgcgctg acggccggcc ggcgaccgtc gcgtcgcggc cgtcgtgcga
     3121 cggttgccgt ggtctccgca ggtccgcccg gcgcggatgc tgcgccgcgg caatctttcg
     3181 gcgaaaaccc gctcggagat acccgtacga gcggtcgggc atgcgacgcg gctcagtccg
     3241 gcgcaagccg cccgcagggc ctcgccaaac acgtctagac aggccagatc gcttgttttt
     3301 aaaggcttta ctgcgtagcg tatgctcact tcgatacacg actagaggaa tccacccgac
     3361 cctcgaggct tgaggtttcg agggggttcc ggtaccattc gcgtttgaga atttcagggt
     3421 cgccacggaa caaggttgcc gcatttgcga gcacccgacc gaggccggaa ccggtcagtc
     3481 agccggacga acggggtgct gtgttgatca tcgagaagga cgtcgcgaag aacgtcaacg
     3541 cggccgcccc gtgcggtcag cgtgccggat cagcgccgag cctgtctctc cgtggccagc
     3601 gagccggttc gctgctcaag cgagtcctgg cagcggtcgt cattgccggc gccgcggtcc
     3661 cgggcctttc gccggcgcgg tccgcaccgg ggctcggcga tcccgtcacc ttgtccagcc
     3721 tgccgcgcga agcgcagcgg acctggcaac tgatccatag cggcgggccg ttcccttatt
     3781 cgaaggacgg cgtggtcttc ggcaaccgcg agaagcgcct ggagcgcgaa ccgcggggct
     3841 actaccacga atacacggtg ccgacgcccg gggcgcgcga tcgcggcgcc cggcgaatca
     3901 tttgcggcgg cgagcacctg acggaaccag tcgcttgttt ttataccgaa gatcactaca
     3961 acagttttca tcggatcgtg aagtgacacg cagcaagacg cgtgtcgtgg atttctgaga
     4021 ggaacgtggc catgcttttg cagaccgtcc gtcccaacat cgttcagtcg atacgggcgt
     4081 atcgggtcga cgaattgctc gaggcggctc aaggggccgg acagcatttc ctgtacgcga
     4141 acctgaccaa tgcgcagtcc aagcaggacg tgctggagca gatcgcggag gcattcctgt
     4201 tcccggcgca cttcggcaag aacctcgatg cgctctacga cgcgatgacc gatctcgtgc
     4261 acaagtccgg ctcgcagccg ggcttcgtcg tcgtgctgga gcaattgccc gacaacccga
     4321 agttcgaccg tgaatcgcgc gagcaattgc tcgacgtatt ccgcgacgct gcagacttct
     4381 ggggggaacg caagatagcg ttcaggtgct tctattcttt tcagtagccc gctcccagga
     4441 aaacgggtca tgggagcggg cggcagaagc ggccccgatc gtcgagaagc cggccaccaa
     4501 ggccagcagc aaggccaacg cgaacgcgaa cttcggcatg aacatgccgc tgatgagcag
     4561 cgcgttcgat accgccatgt ggctcaacgc cgcctgaggc gttccacgac gcagccttcg
     4621 tcacgacacg gcgccccgcg gggcgccgtt ttcattggat ccgagacagc atgccggact
     4681 tcctgaggcg cctgctgcgc cgcgccgagc cgccgcgggc gacgagcgtg catcccgccc
     4741 tcggcgcagc gctcgtccgc cacgaggcct gggcggacga tgtcgggacg caggatgcca
     4801 ccgtcgcccc gctgcaggtc gacggtgacc cggccccatc ccggcgggtg gccgatgcgc
     4861 gcgatccgca ggcgccgcgc gacgtgttcg tggcgccggg cgggctgggc ctgccgggcg
     4921 tggccctcgc caacatgcgc gatccgcagg cccgggtgca gctctgggaa ctcggcacga
     4981 cgacccccgc gggcggcacc actttcgcgc gcgccctcga actcgttttc ccgccgggcc
     5041 gcgatgccgc gggctggcag gtgcgccagc tcgtgccgct gccgcggctg cagtgcgtcg
     5101 tggtgctgtg ccgcgacgac gatccccgca cggcgctggt cgcggtgctg gacctggcga
     5161 cgcggcgcct gcgcgagctg ggcgtcgccg agccggatcc tttcaccgac ggccccgatg
     5221 gccggccggt gcacctcgcc gcgctgcagg ccggcccgga cgccgtcctg gtgcgctggc
     5281 acagcggccg cgtccgtctc gggcagtggg gtgacgtggc gcaggaggac cgggtcgtgt
     5341 tgttcacgcc gcgcgagcgc gacggcctgc ccgtgctgac tctcgcgctg gacgacggca
     5401 acatccgcgg ctggggcctg cagggcacga cgctgtggct gcatgccatc gacggccggc
     5461 cgcgaccggc gccacgcgaa tacgtctggt cgctggcctt gggcgcggtg ttgtaacgcg
     5521 acgaaacagc cgggcggcct gcatccctct acggcgcgcg gcatcccccg tccgggcggg
     5581 ttgctcgaat cgacggatcg atcacaatcc gcgcacgccg cctgccgccg agcaggccgg
     5641 ccaccggcca agacgccgtt cgcgcaaggg aaagggccct tgcgcagagt ccgcgatcct
     5701 cccaggacgt cgcgggcgcg ccgtcgagtg ctctcgatgc ctcgagctca ggtccgccgt
     5761 ccgcgcgggc cgcgcccgcc catgtcacgc tccccctccc cctcgccgtc attcgcggcg
     5821 ccacttccgt cccggaccgc ggcccgtccc gcgcaggcgc tcgcgcgcgc gctccggatc
     5881 gcaggatgga cgctcgccgc gatgctcgcg gtcggctgcg aaccggcgca atcctccggc
     5941 agcaatcaag aggacaccat gttcttcgga cttttcaaga gcaagtctcc ctccaccgtc
     6001 gacccgtcgg tcggcgctgc gctggtgcgc gaggcggcgt ttcccaaggg ggagacgcaa
     6061 ccgccggtgc cggcttaccc gcagttgcgc agggtcgacc tcacggcggc acctccggaa
     6121 ggtcggatct cgccgaaggc gatcgaggag cccgccgcag acgcctacgt gacgagcgga
     6181 gccccgggct cgtcggcgct cgtgaacacg tacgaccccg agcgccgctg cgagctgtgg
     6241 acgatcgacg ccaaggatcc gtcgcggctc gtcggccagc ggcagctccg gttcgaggcc
     6301 gatcaatccc gctggatcat gttcggcgtc ggcgacgccg tcgcgcttcc gggcaacaag
     6361 ctcctcgtgc aattgcgcac gttcgacccc ggccccaccg accgggcgta cgtgatcgac
     6421 ctgagcgcgg gaactgcgcg cagcctcggc gtcatccgac cagactggcc ggccggcctg
     6481 cctttccact atctggacag cctgcaactc cggccggatg cggtgctcgc ggtctttcgc
     6541 accgaccaat tgcgcctgcg tgcggagcac tacgtcaacc gattcgacca cctgctgctg
     6601 ttctcgccgc gacatcccga cgggctcgag atcgcccgca tcggcatcga tgacggcaat
     6661 gtccgcggct ggcgcttgat cggcagcaag ctgtggttgc agacgtccga cgatcggggc
     6721 gacgcaccca aggtccacgt ctggtcgctc gacctcacgc gcgtcctctg atccttaccc
     6781 aagcttccaa cgacttccag ggaacaggcg ctacagcctc caacaacgat gaacgaatcc
     6841 gatttcagcg cgctctcgcc tctcgaggcc gcgtgcatcg cgcacaactc gtatttcacc
     6901 ctcaaggact gggtcaaggg cgcccccgcc aagggcatcg agcagacgag taccgtgaaa
     6961 cgcatggtcc tgggcgacgg cgagacacag cacacgctga gcaagcgcga cgtcaacaca
     7021 tcgctcgcca acacgaagct gcgcggggcc aacctcgaag cggtcttcca ggggtcgacc
     7081 gcgggggtga agacaggctt cggctatgtc ctgtccttca accgcaacgg gctcaagcac
     7141 gcggtcatcg cgacgcgagg cacccgcgtg gagcactcga tcgcggacgg cctcgccgac
     7201 ttgcacgccg ccatcacgac cttcggcggc tacggtcagg tgcacgccgg cttctacaac
     7261 gcgttcgcga gcatcatgcc caacctcgcc tcgcaggagc gagccttgat ggacgccgac
     7321 gtcgttcact gcgtcggcca cagcctgggc ggcgcgatcg ccaccctgat cgcggcgcac
     7381 tacgccggca ggcgcggcgc gggcgtcaag ctttacacgt tcggatcccc gcgtgtgggc
     7441 gcctacgcga ccccgcaggc gttcgagtcg atgatcggca aggacagcat ctaccgcgtc
     7501 gcgcacgacc tcgaccctgt gaccctggtc ggacccttcc cgtattcgca cgtcaacggg
     7561 ctgcccgacg accagaacaa catgatgctg cgctcgccga cggcacggtt gatcagcgtc
     7621 gccaaccacg acatgatgca gtacgtggaa tcgatcatgc ccggcggcga gaccggcatg
     7681 aaatgggcaa acatccgccg gatgagcgcc ggcgtcgtcc acgacaatgc ggtactcgcg
     7741 cggtggctgc tgcgcagcgt cgatcagccg agcatgttca cgcagaaggt cgtcgagggt
     7801 ttgagcgtgc tgctcaaggc tttcgcgcat ttcctgcaaa tgcagcaggt caccagcgca
     7861 atcatctccg gcatgactgc gatcgacatg ttctccgccg ctctcgcgaa gggcatcgaa
     7921 cgcgtcgccg gcacgagccc ggaaatcatg gccagtctga gcatcgccgc tgcctgggct
     7981 cgcgtcgcgg tggtcggcat gaagttcacg gccgcggtga tcaaggccat catgatgcgg
     8041 atgatggccg tgctcaagcc catcgcgagc cgcgccctca cactggtggg agccggtgat
     8101 ccgctacccc tcgtactggc gggcgcgacg gtgatcgccg gcaccatgct gggctgaccc
     8161 gggtcgacac ggcatggagc acagcatgga tcgccaatcg ttcgaacacc tctacctgca
     8221 cgccaacagc atcgccacgc tggccttcct gcgcgcgatg gcccgcatca tcgccatgcc
     8281 agcgcctacc gagccatctc ccgcgatgca agaattcgcc gcggccatcg agcaccagat
     8341 gcagcacccg gagaccttcg actggtcggt ctacgacggc atcgacgagg gcgtcgacga
     8401 cctgatccgg caacgcgagg aagccgaagc ctccggcctc cccttcttcg gcgactgcgt
     8461 cgcaatggcc gaggaagacg aggccgaggg cgagatgccg gacgtcgggc gcttcctgga
     8521 gcgcgcgcgc gcggccgcgg cggcgctggc ggcgcgcatc gacgacagct tcgcccgcgt
     8581 cgaccacgcc gcgctggccg acgcgctggc caccttgccg cgcgacctgc cgccgctgac
     8641 gaccgcactc tcggccgaag acctcgagcg ctggatcgtc ctgtacgagg gcgaacacga
     8701 gggtctgcag cgcgccgtcg aggagacgcg caagcccgag ccggtgctct gacgccggcc
     8761 ctgccggccg cgtcaggccg ccttcacggc acgtgccagc gcgaggtact cgtccacggg
     8821 cacctcctcg gcgcggcgct ggacgtcgaa cgtgccgtcg aagccgcgct cgtcgagcca
     8881 cttgcccagc gtgttgcgca ggatcttgcg gcgctgcgag aacgcgaccg ccaccagctc
     8941 cccgagcagc accgggtcgg cccgggcggc gacctccgcc ggccggggac gcatcgcgac
     9001 gatggccgag tcgaccttgg gcggcgggtc gaaggcgtcg ggcgcgacgt cgaacaggtg
     9061 ctcgatctcg tagcgccact gcagcatcac cgacagccgg ccgaagtcct tgccgccggg
     9121 cccggcggcc atgcggtcga cgacctcctt ctgcagcatg aacacctggt cgaccacgcg
     9181 cggcgcggcg gcgatcaggt ggaacaggat cggcgacgag atgttgtagg gcaggttgcc
     9241 gaccacgcgg atcgggccgc cgaggcggtc ggcaagcgcc gcgaagtcga ccttgagcac
     9301 gtcggattcg acgacgtcga ggtcgccgcg ctggcgcagc cgcgcggcga ggtcgcggtc
     9361 gagctcgacc accgtcaggg cgtcgcagcg ggcgcgcagc gggatcgtca tcgcgcccag
     9421 gccgggaccg atctcgacca ggcgctcgcc gggccgggga tcgatcgccc ggacgatgcg
     9481 ctcgatgacg tccgtggccg tcaggaagtg ctggccgaag cgcttgcgcg ggatgtgcgc
     9541 cacggtctta ctgcggcggg tcgcggtatt cgatgaaggc cttggcgcgc aggtcggcca
     9601 cccactcctg gtaggccggc tcgaacttct gctcgcgcag cgcgttcaag gcctgctcgc
     9661 gcagctgctt gggctcgatc acctgctcgc ggcggtccag cacctggatc aggtggacgc
     9721 cgaagcgcga gcgcaccggc atcgacaggc cgccgaccgg cagcttgtcc atcgcctgct
     9781 cgaactcggg caccagcgag ccgggcgaga tccagccgag gtcgccgccc tgcgcggcgc
     9841 tgccgtcctc ggagtactgg cgcgcgacct cctcgaacgt gcgcgcgccg gaggcgatca
     9901 gctggcgcag gtcgagcatg cggcgctcgg cggcgtccag gctcagctcg gccgacggcc
     9961 gcagcacgat gtggcgcacg cgggtctgga cgatcgtgat gcccgactcc tcgccgcgcg
    10021 acaacacctt caggatgtgg aagcccgcgc cgctgcggat cacgggcgtg gtgccgccga
    10081 ccttcacgcc ctcgatcgcc ttcgcgaaca ggtcgggata gcggtccagc gggcgcgagc
    10141 ccatctcgcc gcccttggcg cggttggtgt cctccgacat ctcgcgagcc acctgctcga
    10201 aaggctcgcc gttgttgacg cgggcaagcg cctgctcggc gcgcgccttg cgctcggcga
    10261 cctcctcggg cgacgccttg tcgggcaccg tcacgaggat ctgcgcgatg tcgacctgcg
    10321 gcgcggtgga gtggccttcc ttgagcttgg cgatgtagtc cgcgacctcc gagtcgctca
    10381 cgtggatgtg cgacaccacc tcgtgctcgc gcacgcgctc gatcgtgatc tggtcgcgga
    10441 tgttgttgcg gaaggtcgtc cagtccatgc cctgctggcg caggcggtcg cgcagctgcg
    10501 ccgacgtgag cttgttgccg gcggcaatgt tgtcgatcgc gcggtcgacc tcggcgtcgt
    10561 ccaccttcac gccgatctcg cgcgcgtgcg tcgtcagcac gcgctcctcg atgagctggt
    10621 cgagcgcctg cttgcgcagc tcggtctccg tgggctgcgg gccgcccttc tcggcgtcct
    10681 cctcgcgcac cttggcgagg cgttgcgtca cctcgttgtt cgtgaccgac tcgtcgttga
    10741 cgaccgcgac gatgtagtcg ccagggaccg attccggtcg acgcgccgtc ttgggcgcgg
    10801 gcgcgggggc gggggcggag gccgcggatc cggcggagat cgcgacggtc ttggccggct
    10861 cctgcgcgga cgcgggagcc gcggccgcga aagccagcac gacggccagc atcaaggcga
    10921 ccggggacgg gacaagggac atcgagcgat ctctcattgg gaagtcgtgg acgaggccgt
    10981 ggacgacggg aacagtccgg cgtcgtcgcg cagcggctgg tagccgggga cgttgttctt
    11041 cagcgccttc gtcggcgatc cgaatccgag tgcggacagg ccgttgagtt ccagctgcag
    11101 catgtagtgg ttggtgcttt cgttcacgcc ggtggaggtg cgctcgacga cgaagcgggc
    11161 gatccagcag ttggcgtcgt attccacccc gccgaccgcg taggtcgacc ggcggtcgga
    11221 gacgctgtag tcgatccggc cgacgccgta cagcgtgccg ccgtcgccgc agcgcgatcc
    11281 gcccgagcgg ccggtgccgg gcgcgttgtg gtacagcggc cactggccgg ccacctcgta
    11341 ggactcgctc gagccgcgcg cgaagcggta ggtcaggctg agcgaacgga acggccccgg
    11401 atggtagcgc gcgctgacga tggcgcgctg cgtccagctc gacgacgggt tgatctccac
    11461 cgtgccgtcg tagctccagt gctccatcgc cgaggacgag ccccagatca gcaggtcgga
    11521 gagcttgcgc gtgtcgggcg tgccgtcggc ggtgagcgcc tggtccttga acagcagccg
    11581 ctgcgcgacg cccaggcgca gcagctcgcg gccgttgacc gcgtcgttga agcgcgtcgt
    11641 ggcgccggcg gtcagctggt tctcgtcgga gatgcggtcg atgccggtga actggttgtc
    11701 gctgtagatc gagacctcgt tgaagtcctt gggcgccgtg tcgtacagcg gcagcgccgt
    11761 ctggtcgtgg tacggcgtgt agacgtagag caggcgcggc tccagcgtct gcacgaggtc
    11821 gcggccgaac agcgaggtct ggcgctcgaa gctcgccgaa ctgtcggtcg agaaggtcgg
    11881 gatccagcgc gtcgcggtcg tgcggccgtc gctcatcggc gagtccgtgt ggtacgtcgc
    11941 gccattgatc gacaggcgcg gctcgacgcg cccccaatcg gagacgaagg cccgcgacac
    12001 cgtgccgatc aggtgcacgc gcgtgccgcc ctcgcgcgtg tcgggcggca ggccggcggc
    12061 cgcgaggtcg ccggtggaga ccttgtcgga caggtcgaag cggttgccct cggtctcgaa
    12121 ctcgaagcgc gtgcggtcgt cgagcagcag gctgccgcgc acgcccagct gcggcgagcg
    12181 ctggtagggc acctggatga cggccgccgg atcctcgaga tcctgcagcg tctggaagcg
    12241 ctggatgcgg gcgtacgcgt cgatctcgcc ggcggggccc cagggcatga agcggcgcac
    12301 gcccgacagg tcggtcggca gcaggcgctg cgtgacggcg ctcagctggc cggggaagtc
    12361 cttccagtac gtgtcgtcgg agacgcgctg gacgcgggcg ctgtagctca gccagtcccc
    12421 gcgcacgccc tcgtgcgccc actcgagcga gccgcgggtc ttgtcctcga tcttgtcgtc
    12481 gggcagcagg tacagcgtcg tctggccgag gtcgcccggc tcgaggtagc gcagctcgct
    12541 cgtcagcgtc gggccgcggc gggtgatgta ggcgggcgtg aaggtcgcat cgacgttcgg
    12601 gcgcaggttc cagtagtacg gcacgcccag gccggggccg gagcggttgc tcgcctcggc
    12661 ggtgggcggc agccagccgg acttgcggtc ggccgtgacc gggaacgtca gcaccggcgc
    12721 ggcaaggatg ggcacgccca ggaagcgcag cacggcatgc tcggcgcggc cctggttggt
    12781 ggccatgtcg atgtcgatgc ggtccccctt gatgatccag tcgggctcct ggccggcggg
    12841 caggtcgcgc gggcaggcgg tgtagtcggc ctggtagagc gtcgaatggt cgcgatcctc
    12901 cagctcgatg cgctgcgcct tgccgccggc cttgcgcgag ccgatctcgt actcggtgtc
    12961 cagcagggtg cccgcgtagc gcgtcagctc gaggtcgagg cggtcggcgc tgaaccagtc
    13021 caggccgcgc gcgatgcgca cgtggccctc ggcatgggcg gtgtcgctca gctggtcgta
    13081 gctcaggagg tccgccttga gctcgaggtc ctggcgcttc aggtcgacgc cgccgctcgc
    13141 ctgggcgacg ccgccgatgt ggctgacgac gttgtccgcg gtgagcgtga tctgcggcgg
    13201 cggctggtcg gagccggcga cttccctggc cggcgcgggc gtgggcgtcg gacgcacctt
    13261 gggaagcgcc tgggcgcccg cggatcccgc ggtcgcgcac aacagcgccg ccacggccag
    13321 tggcgcgagg gcggagggag gaaggcgacg acgcgacgag gacagacgca gcacgcgagc
    13381 gggatggcag gggtggagtt cgaagcgcct cgcgccgcac ggacccccgc gacgacggac
    13441 gtgcatcgac gcgcgacgcc gcccggccgg agaccggccc gcctgcggcg cagggggatt
    13501 ccttagaatg cggattatgc attcccgcgc gtcccgagcc cgcattcgat gaacgacctc
    13561 tccgccggcc acgccgccgc gcaacgcctt cccgtcacct gggccgacgc cgagcgcgag
    13621 caggccttcg gccgctggct gcacgcgatc gcggcgaagc accggctcga cccgtcctcc
    13681 ctgcgcctgg catcggccga cgcgagcgcg cggcgctacc tgcgcatcga cgccgtcgcc
    13741 tccgacgacg cggccgtgca cagctacatc gtgatggact cgcccgcggc cgaggacagc
    13801 atcgccgcct tcgtgcacgt cggcgggctg atccgcgccg ccgacctcaa cggcccgcgc
    13861 gtgctcgagg aggaccagcg ccagggcttc ctgctgctgg acgacctcgg cgacacgccc
    13921 tacctcgccg cgctgcagga cgcggacgcc ggcgccgccg atgccctgat gcgcgatgcc
    13981 atcgccgcgc tggtgcagtg gcaactgcgc gtcgaccccg cgacgctgcc cccctacgac
    14041 gaggcgctgc tgcgccgcga gctgcagctg tttcccgact ggtgcgtcgc gcgcgagttc
    14101 ggcatcgcct ggggcgaggc cgagcagaag ctgtgggccg acgtcagccg ccggctgatc
    14161 gacagcgccc tcgcccagcc gcaggtcgcc gtccaccgcg actggatgcc gcgcaacctc
    14221 atggtctgcg agcgcaaccc cggcgtgctc gacttccagg acgccgtccg cggcccgatc
    14281 agctacgaca tcgcctcgct gctgcgcgat gcgttcatct cctgggacga ggagcgcgag
    14341 ctggactggg cggtgcgcta ctggcagcgc gcgcgcgccg ccgggctgcc ggtcgacccg
    14401 gacttcggcg agttctggcg cgccaccgag tggatgggcc tgcaacgcca tctgaagatc
    14461 ctcggcatct tctgccgcct caagcaccgc gacggcaagc cgcgctacag cgaggacctg
    14521 ccgcgcttct tcggctacgc cacgcgcgtg gcgcaccgct acgcgccgct gcgcgggctg
    14581 ctgaagctgc tcgagccgat gtcgggccag gccgtcgccg cgggcttcac gttctgacgc
    14641 cgcggccggg gccatgagaa acgggcgccg cggcgcccgt cgtcatgtcc ttcgagtccg
    14701 gaggatcaga agctttccca gtcgccgccg tcgccggtcg acggcgcggc ggcgaccgcc
    14761 accggtgcct cgacgctcgg cgctgccggc acgaccgggg
//
LOCUS       DASVLN010000140         5496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_60506, whole genome shotgun sequence.
ACCESSION   DASVLN010000140 DASVLN010000000
VERSION     DASVLN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60506"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VF453_03080"
     CDS             complement(<1..417)
                     /locus_tag="VF453_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HEX7686659.1"
                     /translation="MNREMLMLVDAISREKTVERELVFGAVESALASATKKLHGPDVD
                     IRVSVDRETGEYDTFRRWHVVPNEAGLQIPDAEILLFEAQEQIADIEVDDYIEEPIES
                     VPIGRIGAQAAKQVILQKIRDAEREQLLNDFLSRGER"
     gene            complement(414..1037)
                     /gene="rimP"
                     /locus_tag="VF453_03085"
     CDS             complement(414..1037)
                     /gene="rimP"
                     /locus_tag="VF453_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX7686660.1"
                     /translation="MTVTAHNSSWSAAVERTVTGLGFDLVDVERSGGGLLRVTIDRVP
                     GRSYPTGESEFVLVEDCEAVTRQLQYVLEVENCDYARLEVSSPGLDRPLKLSDAEFAR
                     FAGQQAEVTLRQPFQGRKKWSGELQAREGGWRLVLPKAPVSKTRAKAMAAAGIAPEAE
                     HTLDFALEELREARLVPVLDFKGRGRQARSDAGPQAGDQSDDGGQEE"
     gene            1472..2272
                     /gene="fabI"
                     /locus_tag="VF453_03090"
     CDS             1472..2272
                     /gene="fabI"
                     /locus_tag="VF453_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX7686661.1"
                     /translation="MGFLAGKRLLITGVLNNRSIAYGVARACHREGAELAFSYVGERF
                     KERITEFAAEFDSRLVFDCDVGSDEQIAAMFDGLAQAWPEGFDGFVHSIGFAPREAIA
                     GDFLDGLSREAFRVAHDISAYSFPAMAKAALPHLRDRSALLTMTYLGAIRAVPAYNTM
                     GLAKASLEASVRYLAASMGPKKAIRVNGISAGPIKTLAASGIKGFGKLLGVFEENAPL
                     RRTVTIDDVGNVAAFMLSDLAGGVTGEITYVDAGFSQVMGGGAALLDE"
     gene            complement(2335..3312)
                     /gene="glk"
                     /locus_tag="VF453_03095"
     CDS             complement(2335..3312)
                     /gene="glk"
                     /locus_tag="VF453_03095"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX7686662.1"
                     /translation="MNVDSNSGFPRLLGDVGGTNARFALQEAPDAGPTQVVTYKGADY
                     PNFEQAILHYLSQVSGPRPVQGAIGIANPIVGDHVKMTNSSWSFSIEGIRQKAGFSRF
                     LVVNDFTALALSLPALEPAHLRRIGGGTADPASAVGLLGPGTGLGVSGLVRVDGRRTV
                     PLGGEGGHVSLAPANAREDAVVAVLRDRFGHASAERALSGSGLVNLYEALCAIDGVAA
                     RALEPAGVTADATSGADVRCVEAVDMFFAFLGSCAGNLALTLGARGGIYIGGGIPPRL
                     GDWIDRSAFRERFVAKGRFRDYLDAIPTWLIVADTSPALIGAARALDEL"
     gene            complement(3512..5416)
                     /locus_tag="VF453_03100"
     CDS             complement(3512..5416)
                     /locus_tag="VF453_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HEX7686663.1"
                     /translation="MHHPRQLSAAMLLAAGTLAGGAQAADAAPAAAPIERIEPAFWWV
                     GMHDERLQLMVHGPRIADAAPRLEAGSDEGGAVRIESVSRVANPNYLFIDLRIRPDAR
                     PGRLEIVFSRGEQTFRAPYELRAREPGSAQRRGFDSSDVMLCLVPDRFADGDPRNDHV
                     AGYTDVVDRSDDRKARHGGDIQGIVDHLDYIRGLGVTMLWPTPLTENAQPEYSYHGYA
                     ATDTYKIDPRFGTNEDYVRLVHAAREKGIGVVMDVVLNHIGSHHWWMRDMPTPDWIGF
                     DGRYVQTQHARTTTSDPYASKADSRIYTDGWFGPNMPDMNQRNPFLATYQIQNAIWWI
                     EYAGLAGLRVDTWGYSDQAFLQEWTTRVRAEYPNLNIVGEEWTGQPAVLMHWLRGGVG
                     GEPVAGRLPSAMDFPLNQTLRRALTAEDTLHTGLMELYEALVNDVLYPEPSKMVLFEG
                     NHDMPRLYSALGDDADLTKMALAYVMTMPRVPQLYYGTEILMTSPTVRDDGKVRQDFP
                     GGWPGDRVDAFTGEGLTPAQLDMQRFVRTLGTWRRTQTTIHHGALTHFVPEHGTYVWF
                     RHDAHSVVMVAINKNHADATLDMARFREVLPAPVAAREVISGRQVELGATVTLPPRSV
                     RIFEVLRRPG"
BASE COUNT          808 a         1916 c         1921 g          851 t
ORIGIN      
        1 gcgctcgccg cgcgacagga agtcgttgag cagctgctcg cgctcggcgt cgcggatctt
       61 ctgcaggatg acctgcttgg ccgcctgcgc gccgatgcgg ccgatcggca ccgactcgat
      121 cggctcctcg atgtagtcgt ccacctcgat gtcggcgatc tgctcctggg cctcgaacag
      181 caggatctcg gcgtccggga tctgcaggcc ggcctcgttg ggcacgacgt gccagcggcg
      241 gaaggtgtcg tactcgcccg tctcgcggtc gacggagacg cggatgtcca cgtcgggccc
      301 gtgaagcttc ttggtggccg acgcgagcgc ggactcgacc gcgccgaaca cgagctcgcg
      361 ttcgacggtc ttctcgcgcg agatcgcgtc caccaacatc agcatttcgc ggttcattct
      421 tcttgacctc cgtcgtcgga ttgatcgccg gcctgcggcc cggcatcgga gcgggcctgg
      481 cggccgcgcc ccttgaagtc cagcaccggc acgagccggg cctctcgcaa ttcttccagc
      541 gcgaagtcga gcgtgtgctc ggcctcgggc gcgatccctg ccgccgccat cgccttcgcc
      601 ctcgtcttgc tgaccggcgc cttcggcagc accagccgcc agccgccttc gcgcgcctgc
      661 agctcgccgc tccacttctt gcggccctgg aagggctggc gcagcgtgac ctcggcctgc
      721 tgccctgcga aacgggcgaa ctcggcgtcg ctcagcttga gcggacggtc gagcccgggc
      781 gacgacacct cgagccgggc gtagtcgcag ttctcgacct cgagcacata ttgcagctgc
      841 cgcgtgacag cttcgcagtc ctcgaccagc acgaactccg actccccggt cgggtacgag
      901 cgccccggca cgcgatcgat cgtcacgcgc agcagcccgc cgccggaacg ctcgacgtcc
      961 accaggtcaa atcccagacc ggtgacggtg cgttcgacgg ctgcggacca gctcgagttg
     1021 tgtgcggtga ctgtcaagaa agcgttcggt gttcgatgac gaaagggacg gccaaaaagt
     1081 gacaaaggcc cctgaggacc cttgcacctg catcggcgcc gccgccgttt cgggcggcgc
     1141 cgcctggaga gttctatttt cgcttcggcc ctgctctacc gggacttaat tcacgctccg
     1201 ggcacagcgg caaaaaaaat gggcagaacc agatccgccc acctcttcct cggctgccgc
     1261 ccgaccctcg tgcactgcac gcggaccgga cgtcccggtt gcgctgaaaa tccccttttc
     1321 cgccgggcgc gccccgctaa actgcggggc ttgaacgtcg gatgccgggt atccagtact
     1381 tcagcgaagc tcgtattata gcctgcaaga cccgtacccg caagattggg gtgcgacaat
     1441 ctccctccac gcaacaattc ccggagaccg catgggattc ctcgccggca agcgcctgct
     1501 catcacgggt gtcttgaaca accgctccat cgcctacggc gtcgcccgcg cctgccaccg
     1561 cgaaggcgcc gaactggcct tcagctacgt gggcgagcgc ttcaaggagc gcatcaccga
     1621 gttcgccgcc gaattcgact cccgcctcgt gttcgactgc gacgtcggca gcgacgagca
     1681 gatcgccgcc atgttcgacg gcctggccca ggcctggccc gagggcttcg acggcttcgt
     1741 ccactcgatc ggcttcgccc cgcgcgaggc gatcgcgggc gatttcctcg acggcctgtc
     1801 gcgcgaggcc ttccgcgtcg cgcacgacat ctcggcctac agcttcccgg cgatggccaa
     1861 ggccgcgctg ccgcacctgc gcgatcgcag cgcgctgctg acgatgacct acctgggcgc
     1921 catccgcgcc gtgccggcct acaacaccat gggactcgcc aaggcctcgc tggaggccag
     1981 cgtgcgctac ctggccgcga gcatgggccc gaagaaggcg atccgcgtca acggcatctc
     2041 cgccggcccg atcaagacgc tcgccgcctc cgggatcaag ggattcggca agctgctggg
     2101 cgtgttcgag gagaacgccc cgctgcgccg cacggtgacg atcgacgacg tcggcaacgt
     2161 cgccgcgttc atgctgtccg acctggccgg cggcgtgacg ggcgagatca cgtacgtcga
     2221 tgccggcttc agccaggtga tgggcggcgg cgccgcgctg ctggacgagt gatccggctg
     2281 cggcccacgg gaaagcgcgc cgggcggcgc gcttttttca tgggcggcgg ccgatcagag
     2341 ctcgtcgagc gcccgcgccg cgccgatcag cgccggcgag gtgtcggcga cgatcagcca
     2401 ggtcgggatg gcgtcgaggt agtcgcggaa gcggcccttc gcgacgaacc gctcccggaa
     2461 ggccgagcgg tcgatccagt cgcccaggcg cggcgggatg ccgccgccga tgtagatgcc
     2521 gccgcgcgcg cccagcgtca gcgccaggtt gccggcgcag ctgccgagga acgcgaagaa
     2581 catgtcgacc gcctcgacgc agcgcacgtc ggcgccgctc gtcgcgtcgg cggtgacgcc
     2641 ggcgggctcg agcgcgcgcg ccgccacccc gtcgatggcg cacagcgcct cgtagagatt
     2701 cacgaggccc gaaccggaga gcgcgcgctc ggccgacgca tggccgaagc gatcgcgcag
     2761 caccgcgacg accgcgtcct cgcgcgcgtt cgcgggcgcg aggctgacgt ggccgccctc
     2821 gccgcccagc ggcacggtgc gcctgccgtc cacgcgcacg aggcccgaga cgcccaggcc
     2881 cgtccccggc ccgagcaggc cgaccgcgga agcgggatcg gccgtcccac cgccgatccg
     2941 ccgcaggtgc gccggctcga gcgcgggcag cgacagcgcg agcgccgtga agtcgttgac
     3001 gaccaggaag cgcgagaagc ccgccttctg ccggatgccc tcgatcgaga acgaccagct
     3061 cgagttggtc atcttcacgt ggtcgcccac gatcgggttg gcgatgccga tggcgccctg
     3121 caccggccgc ggcccgctca cctgcgagag gtagtgcagg atcgcctgct cgaagttcgg
     3181 gtagtccgcg cccttgtacg tgacgacctg cgtgggcccg gcatccggcg cctcctgcag
     3241 cgcgaagcgg gcgttggtgc cgccgacgtc gccgagcagg cgcgggaaac cggagttgga
     3301 atcgacattc atggaaacgg caggactcgc gggtccgatg gaaggggttg ggggggcatg
     3361 cgcgggcaca ctcgcggcga agccgcaaat gtagtttgcc tacgcatttc ccgcaacacc
     3421 cccgagccac cgggagtttc cgcctcaacc ggtgcacgcc tgcgtcaccg gcctttcacg
     3481 aaagccgtag ttcgaataca cccccgcacg ctcagccagg gcgccgcagc acctcgaaga
     3541 tccgtaccga gcgcggcggc agcgtgacgg tcgcgccgag ctcgacctgc cggccggaga
     3601 tcacctcccg cgccgccacc ggcgccggga gcacctcccg gaagcgcgcc atgtccagcg
     3661 tcgcgtcggc gtggttcttg ttgatcgcca ccatcacgac cgagtgcgcg tcgtggcgga
     3721 accagacata ggtgccgtgc tcgggcacga agtgcgtcag cgcgccgtgg tggatggtcg
     3781 tctgcgtgcg gcgccaggtg ccgagcgtgc ggacgaagcg ctgcatgtcc agctgcgccg
     3841 gggtcaggcc ctcgccggtg aaggcgtcga cccgatcgcc gggccagccg ccggggaaat
     3901 cctggcgcac cttgccgtcg tcgcggacgg tcgggctggt catcaggatc tcggtgccgt
     3961 agtacagctg cggcacgcgc ggcatggtca tcacgtaggc cagcgccatc ttcgtgaggt
     4021 cggcgtcgtc gccgagcgcg ctgtacagcc gcggcatgtc gtggttgccc tcgaacagca
     4081 ccatcttcga cggctcgggg tacagcacgt cgttgaccag tgcctcgtag agctccatca
     4141 ggccggtgtg cagcgtgtcc tcggcggtga gcgcgcggcg cagcgtctgg ttgaggggaa
     4201 agtccatcgc gctgggcagg cggccggcga ccggctcgcc gccgaccccg ccgcgcagcc
     4261 agtgcatcag caccgcgggc tggccggtcc attcctcgcc gacgatgttg aggttcggat
     4321 actcggcccg cacgcgcgtc gtccactcct ggaggaaggc ctggtcggag tagccccagg
     4381 tatccacgcg caggccggcg aggccggcgt actcgatcca ccagatcgcg ttctggatct
     4441 ggtaggtcgc gaggaagggg ttgcgctggt tcatgtcggg catgttgggc ccgaaccagc
     4501 cgtcggtgta gatgcgcgaa tccgccttgg aggcgtacgg gtcgctggtc gtcgtgcggg
     4561 cgtgctgggt ctggacgtag cggccgtcga agccgatcca gtccggcgtg ggcatgtccc
     4621 gcatccacca gtggtgcgag ccgatgtggt tcagcacgac gtccatcacg acgccgatgc
     4681 ccttctcgcg cgcggcgtgc acgaggcgca cgtaatcctc gttcgtgccg aagcgcgggt
     4741 cgatcttgta ggtgtcggtc gccgcgtagc cgtggtacga gtactcgggc tgcgcgttct
     4801 cggtcagcgg cgtcggccac agcatcgtca cccccaggcc ccggatgtag tcgaggtggt
     4861 cgacgatgcc ctggatgtcg ccgccgtggc gcgccttgcg gtcgtcggag cggtcgacga
     4921 catcggtgta gccggcgaca tggtcgttgc gcgggtcgcc gtcggcgaag cggtcgggga
     4981 cgaggcacag catcacgtcg gagctgtcga agccgcgccg ctgcgccgag cccggctcgc
     5041 gggcgcgcag ctcgtagggc gcgcggaagg tctgctcgcc gcggctgaac acgatctcga
     5101 gccggcccgg gcgcgcgtcc ggccggatgc gcaggtcgat gaagagatag ttcgggttgg
     5161 cgacgcgcga gaccgactcg atccgcactg cgccgccctc gtcgctgccg gcctccaggc
     5221 gcggggcggc gtcggcgatg cgcgggccgt gcaccatcag ctgcaggcgc tcgtcatgca
     5281 tgccgaccca ccagaacgcg ggctcgatgc gctcgatggg cgcggcggcg ggggcggcat
     5341 cggccgcctg ggcgccgccg gcgagcgtgc ccgcggcaag cagcatggcc gcggagagtt
     5401 gtcggggatg gtgcatggat ccttgtctcg tggtgtggtt gttcagggcg acacgaagac
     5461 cgcggcggtg cgggccggga tcgtgaaggt ggcggt
//
LOCUS       DASVLN010000141         8878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_114520, whole genome shotgun sequence.
ACCESSION   DASVLN010000141 DASVLN010000000
VERSION     DASVLN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8878
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114520"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /locus_tag="VF453_03105"
     CDS             complement(<1..1858)
                     /locus_tag="VF453_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686664.1"
                     /translation="MPKTFASPSKTFAAGLRAAPRWALVALTAVACVGLRAALQPALG
                     DQMPFVVAYPAIALAASMWGIPGGLAVAATAAIAAVIPWIPPTIPVADQPRHVGSFAV
                     VAIFMCLVCNLQARRAGASADTDAGADTPLTSWLRAVLWGSMLIPLTAFLAAAWWGYE
                     NASAEAVATARRAGVLVRDHARHTLDVAASIARRADAAASFTDDEVRANEHDIHVRLA
                     DMVAGVPSIVNLNIWDADGQALARSDRYPAASGTVPVADRAYFIAQKPPGARLGVSEV
                     LLGRQTGRELMNVSIRRSSADERFRGIVAVSLSPRFFRDYFRSLATDTPKLARFALVR
                     TDGTVLAAWPDEPVGTRRSPDDQVLARIAVGEASGDAVVRSRDGDVRFASFQRVGSYP
                     LYVVAGVGRRAMFAGWLHYVGLLAALLAPVTAGLTWVSLVAIRKTRREQAVSAQLVEA
                     QERRAAAERAVMEAQKLETLAQLTGGVAHDFNNLLAIVSSSLHVHRHLHPELAEERHL
                     LAMGRAVQSGTRLTRQLLSFSRRQALRPETVDLRAWLPGAEGLLRSTLGSRIDMEIEV
                     EPDTRPVRVDLAELELSLINLALNAKHAMPAGGRLRIVARNEPAGGGAPPRVA"
     gene            1975..2451
                     /locus_tag="VF453_03110"
     CDS             1975..2451
                     /locus_tag="VF453_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7686665.1"
                     /translation="MKKHDFSHRFGFVVNEVGRLYGRQFDQISRVELGLSRAQCRLIA
                     QVASNEGDRPWTQAELAQRLELTPMGVATLCDRMEAAGWIVRRPSATDRRANAIELQP
                     RAEDALAKAIAVSDRVQESALAGFTAAERRQLLAMLQRVRANLSAAPAAPAAGDDQ"
     gene            2576..4135
                     /locus_tag="VF453_03115"
     CDS             2576..4135
                     /locus_tag="VF453_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7686666.1"
                     /translation="MSAQGDHASIPHRGMITVSIMLATIMQALDTTIANVALPHMQGS
                     LQSSQDQISWVLTSYIVAAAIATPLTGWLCSAFGRRRVFLAAVLGFTVASALCGMAGS
                     LWEIVAARLLQGLFGAALVPLSQAVLLDINPKEKIGQAMAIWGAGIMVGPILGPMLGG
                     WLTENADWRWVFYINLPVGALAFYGIARFLPESRPEGIRLDLFGFATLSLAIGLLQMF
                     LDRGEQLDWFDSPEIRIEAIGCLIAFLFFLAHTFTIEGRSFFDRRLLLDANFVSGLVL
                     AFIVGLVLYATMALLPTLLQGLLGYPVVYTGMVMAPRGVGTMVAMLLVGRMLHVIDAR
                     WIMALGFALTALALWIMTRMTLDMGSSLIVASGIIQGLGIGFTFVPLTAVAFATLAPQ
                     LRNDGTPIFSLLRNIGSSVGISIVQAMLTSGTARAHAELAVDINRANLVVQGLPRLLD
                     PATPSGRAILDLEVNRQAAMMAYLGDFRFMLALTVLSMPLLLLIRKPRPAPAKPGEDA
                     VVAEAELLHEV"
     gene            4314..5753
                     /locus_tag="VF453_03120"
     CDS             4314..5753
                     /locus_tag="VF453_03120"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEX7686667.1"
                     /translation="MKNDLPPAPTSEEIATALQAALERQRAAYFEHPVPTLAERKADL
                     RTLLRFIRDHKQALCDAISADYGHRSQHETLLAEVFPAMDGITHAIGQLRKWMRPQRR
                     AVDLRNFLGARNRVIPQPLGVVGIIVPWNFPLHLSLIPLTYVFAAGNRAMVKMSENSR
                     HLAALLIEKMPAYFPPEKLQFFDETGGVGIEFSKLKFDHLLFTGSGATGRSVMAAAAA
                     NLVPVTLELGGKAPAIVCDDFPLRLAAERILFAKLLNAGQICTTVDHAWLPRARIDEF
                     VALAGEIVLQRFGSMASADYTSIIDERAFERLLAALEDARARGARLVQLLPGEPFDRA
                     TRKIAPHLVLDAPADCELLQREIFGPILPLRAYDRLEDVVHAINAGARPLAIYPFSYD
                     GQRVQMLLDRVMSGGVSVNEALFHVAQHDLPFGGVGESGMGHYHGREGFDTFSKLRPV
                     FYQARVSGMKFMAPPYGRFANALLGFLTK"
     gene            complement(5781..6536)
                     /gene="msrA"
                     /locus_tag="VF453_03125"
     CDS             complement(5781..6536)
                     /gene="msrA"
                     /locus_tag="VF453_03125"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7686668.1"
                     /translation="MTRFSRAATLPPSRRLRPFAALLATGAVVFAATHFIGTAHAAET
                     AVAIPPPAVDEPANPARHAETAVFAGGCFWGVQGVFQHVKGVTNAVSGYSGGAKSTAE
                     YEKVGTGLTGHAESVQVTFDPAQVSYGKLLQVYFSVAHNPTELNYQGPDHGSQYRSAV
                     FPLDDAQRKVATAYIAQLDAAKAYGKPVVTRVEPFKGFFPAEGYHQNFLALNPDHPYI
                     VYNDLPKVADLKRLFPEQYMDKPVLVPNVVASK"
     gene            complement(6566..7069)
                     /gene="msrB"
                     /locus_tag="VF453_03130"
     CDS             complement(6566..7069)
                     /gene="msrB"
                     /locus_tag="VF453_03130"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7686669.1"
                     /translation="MPIARRTALLSGAQFVLGGGLVALVAAGVRARAAAPATHYEVEM
                     TDAQWKAKLSPRAYAVLRHEATETPFTSPLNSEHRKGTFSCAGCALALFSSDTKFDSG
                     TGWPSFWQPLPNAVATERDLSLGMVRDEVHCRRCGGHLGHVFDDGPKPTGLRYCMNGV
                     AMTFTPA"
     gene            7264..8016
                     /locus_tag="VF453_03135"
     CDS             7264..8016
                     /locus_tag="VF453_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7686670.1"
                     /translation="MSTSRLDDPAVRTAVAPTGRLRASINLGNPILANADPATGRARG
                     VSVDLAHALAARLDMPLETIEFTAAAKSVDAVAGEAADVGFFAIDPARGASIAFTDAY
                     VLIEGAYLVRADSTLQANEEVDRAGTRIVVGQGSAYDLYLTREIGAARFVRVPTGPAI
                     VETLAAGGADVAGGIRQALADIARERPGLRLLPGRFMVIRQAMGLPRSRGEAAAAALS
                     AFVEEMKASGFVADALARHGISGASVAPPADA"
     gene            8046..8552
                     /locus_tag="VF453_03140"
     CDS             8046..8552
                     /locus_tag="VF453_03140"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7686671.1"
                     /translation="MDSPNALPRHQTSMTVLMTPDMANFSGNVHGGTILKLLDQVAYA
                     CAARYAARYVVTLSVDQVVFREPIHVGELVTFLACVNHTGTSSMEVGVKVLAEEIRTQ
                     RVRHANSCFFTMVALDDDRKPVAVPPLVPATPEERRRHAAAELRKVLRAEYRSRFEAV
                     RQANPDAA"
BASE COUNT         1209 a         3266 c         3148 g         1255 t
ORIGIN      
        1 tcgccacgcg cggcggcgcg ccgccgccgg ccggctcgtt gcgggcgacg atgcgcagcc
       61 ggccgccggc gggcatcgcg tgcttcgcgt tcagggcgag gttgatcagc gagagctcga
      121 gctccgcgag gtcgacgcgc accggccgcg tgtcgggctc cacctcgatc tccatgtcga
      181 tgcgcgagcc gagcgtggag cgcagcaggc cctcggcgcc cggcagccag gcgcgcaggt
      241 cgacggtctc cgggcgcagc gcctggcggc gcgagaacga cagcagctgg cgcgtgagcc
      301 gcgtgccgga ctgcaccgcg cggcccatcg cgagcaggtg ccgctcctcg gccagctccg
      361 gatgcaggtg gcggtgcacg tgcaggctgc tggagacgat ggccagcagg ttgttgaaat
      421 cgtgggcgac gccgcccgtc agctgcgcga gcgtctcgag cttctgcgcc tccatcactg
      481 cgcgctcggc ggcggcgcgg cgctcctggg cctcgaccag ctgggcggag acggcctgct
      541 cgcgccgcgt cttgcggatc gccaccagcg acacccacgt caggcccgcg gtgacgggcg
      601 cgagcagcgc ggcgagcagc cccacgtagt gcagccagcc ggcgaacatc gcgcgccgtc
      661 ccacgcccgc gacgacgtac agcggatagc tgcccacccg ctggaagctc gcgaagcgga
      721 cgtcgccgtc gcgcgagcgc acgaccgcgt cgccggaggc ttcgccgacg gcgatgcggg
      781 cgagcacctg gtcgtccggc gaccgccgcg tgcccaccgg ctcgtcgggc cacgcggcga
      841 gcaccgtgcc atccgtgcgc acgagcgcga agcgcgcgag cttgggcgtg tcggtcgcga
      901 gcgagcggaa gtagtcgcgg aagaagcggg gcgacagcga gaccgcaacg atgccgcgga
      961 agcgctcgtc ggcggagctg cgccggatcg agacgttcat cagctcccgc ccggtctggc
     1021 gcccgagcag cacctcgctc acgcccaggc gcgcgccggg cggcttctgg gcgatgaaat
     1081 acgcgcgatc ggcgaccggc accgtgccgc tcgcggccgg atagcggtcg ctgcgcgcca
     1141 aggcctggcc gtcggcgtcc cagatgttga ggttcacgat cgacgggacg cccgcgacca
     1201 tgtcggccag gcggacgtgg atgtcgtgct cgttcgcgcg cacctcgtcg tcggtgaagc
     1261 tcgcggccgc gtcggcgcgg cgtgcgatcg aggcggccac gtccagcgtg tgccgggcat
     1321 ggtcgcgcac cagcacgccc gcgcggcgcg cggtggcgac ggcctcggcg ctcgcgttct
     1381 cgtagcccca ccaggcggcg gcgaggaagg ccgtcagggg gatcagcatc gacccccaca
     1441 gcacggcgcg cagccacgag gtgagcggcg tgtcggcgcc cgcgtcggtg tccgccgagg
     1501 cgcccgctcg ccgcgcctgc aggttgcaga cgaggcacat gaagatcgcc acgaccgcga
     1561 acgagccgac gtggcgcggc tggtccgcga ccgggatcgt gggcgggatc cagggaatca
     1621 ccgccgcgat ggcggccgtc gccgccacgg cgagtccgcc ggggatcccc cacatcgacg
     1681 cggccagggc gatcgccgga taggccacca cgaacggcat ctggtcgccg agcgccggct
     1741 gcagcgccgc ccgcaggccg acgcaggcca ccgcggtgag cgcgaccagg gcccagcgcg
     1801 gcgcggcgcg caggcccgcc gcgaaggtct tggacggact ggcgaacgtc ttcggcatgg
     1861 cgccggttgc ggaagcggtg gtggagaccc cgcgatggta gcggcccgcc gccgacccgc
     1921 gcgggcgtgg ccccgcgccc tcagttaata aactggttta tcatatatcc ctatatgaaa
     1981 aagcacgact tttcgcaccg cttcgggttc gtcgtgaacg aggtcgggcg cctgtacggt
     2041 cgccagttcg accagatctc gcgcgtggag ctcggcctgt cgcgtgcgca gtgccgcctc
     2101 atcgcgcagg tggcttccaa cgagggcgac cggccgtgga cgcaggccga gctcgcgcag
     2161 cggctcgagc tcactccgat gggcgtcgcg acgctgtgcg accgcatgga ggctgccggc
     2221 tggatcgtgc gccgacccag cgccaccgac cgccgcgcca acgcgatcga gctgcagccg
     2281 cgggccgagg acgcgctcgc caaggccatc gccgtcagcg accgcgtgca ggagagcgcc
     2341 ctggccgggt tcaccgcggc ggaacgccgg cagctgctcg ccatgctgca gcgcgtgcgg
     2401 gccaacctct cggcagcgcc cgcggcgccc gccgcggggg acgatcaatg agcccccgcc
     2461 cggccgatcc gaagggggct cgcaccggag tccgcaggac gcaggttgcc ctatcctccc
     2521 gcccggccgg tccgaaggcg gctcgcaccg cggtccgcag gacgaaggct gcctcatgag
     2581 cgcgcagggc gaccacgcca gcatccccca ccgcgggatg atcacggtct cgatcatgct
     2641 cgcgaccatc atgcaggcgc tcgacacgac catcgccaac gtcgcgctgc cgcacatgca
     2701 gggctcgctg cagagctcgc aggaccagat ctcctgggtg ctgacgtcct acatcgtcgc
     2761 cgccgccatc gccacgccgc tgaccggctg gctgtgctcg gcgttcggac ggcggcgggt
     2821 cttcctcgcg gcggtgctcg gcttcaccgt cgcctccgcg ctgtgcggca tggcgggctc
     2881 gctgtgggag atcgtcgcgg cgcggctgct ccagggcctg ttcggcgctg cgctggtgcc
     2941 gctgtcgcag gcggtgctgc tggacatcaa cccgaaggag aagatcggcc aggcgatggc
     3001 catctggggc gcgggcatca tggtcggccc gatcctgggc ccgatgctgg gcggctggct
     3061 gaccgagaac gccgactggc gctgggtctt ctacatcaac ctgccggtgg gcgcgctcgc
     3121 cttctacggc atcgcccgct tcctgcccga gagccggccc gagggcatcc ggctggacct
     3181 gttcggcttc gcgacgctca gcctcgcgat cggcctgctg cagatgttcc tcgaccgcgg
     3241 cgagcagctc gactggttcg actctccgga gatccgcatc gaggcgatcg gctgcctgat
     3301 cgcgttcctg ttcttcctcg cgcacacgtt cacgatcgag ggccgctcgt tcttcgaccg
     3361 ccggctgctg ctggacgcca acttcgtctc cggcctcgtg ctcgccttca tcgtcggcct
     3421 cgtgctgtac gcgacgatgg cgctgctgcc gacgctgctg cagggcctgc ttggataccc
     3481 cgtggtgtac accggcatgg tgatggcgcc gcgcggcgtc gggacgatgg tcgcgatgct
     3541 gctggtcggg cgcatgctgc acgtcatcga cgcccgctgg atcatggcgc tcggcttcgc
     3601 cctcaccgcc ctcgcgctgt ggatcatgac gcgcatgacg ctggacatgg gcagctcgct
     3661 gatcgtcgcc tcgggcatca tccagggcct ggggatcggc ttcacgttcg tgccgctcac
     3721 cgcggtcgcg ttcgcgacgc tcgcgccgca gctgcgcaac gacggcacgc cgatcttcag
     3781 cctgctgcgc aacatcggct caagcgtcgg catctccatc gtgcaggcga tgctcacctc
     3841 gggcacggcg cgcgcgcacg cggaactcgc ggtggacatc aaccgcgcca acctcgtcgt
     3901 gcagggcctg ccgcgcctgc tcgacccggc gacgccgtcc ggccgcgcga tcctcgacct
     3961 cgaggtcaac cgccaggccg ccatgatggc ctacctcggc gacttccgct tcatgctggc
     4021 gctgacggtg ctgtcgatgc ccctgctgct gctgatccgc aagccgcggc cggctccggc
     4081 caagccgggc gaggacgccg tcgtcgccga ggcggagctg ctgcacgagg tgtgatccgc
     4141 cgatcccgcg attggctcga cgggccaagc attggctccg cgggccagtc gcgcacgcga
     4201 cgttccaggg gtcgcaccct aggacacggg cggcgggcgg gcgcagaatc gtcggctcag
     4261 gcgcgcgcgg ccgccgtcgc gccgccgccc cgacgtgcca tgcgaggacc gccatgaaga
     4321 acgacctgcc cccggctccc accagcgagg agatcgcgac cgcgctgcag gccgcgctcg
     4381 agcgccagcg cgcggcctac ttcgagcacc cggtgccgac gctggccgag cgcaaggccg
     4441 acctgcgcac gctgctgcgc ttcatccgcg accacaagca ggcgctgtgc gacgccatca
     4501 gcgccgacta cggccaccgc tcgcagcacg agacgctgct ggccgaggtg ttcccggcga
     4561 tggacggcat cacgcacgcg atcggccagc tgcgcaagtg gatgcgcccg cagcgtcgcg
     4621 ccgtcgacct gcgcaacttc ctcggcgcgc gcaaccgcgt gatcccgcag ccgctgggcg
     4681 tggtcggcat catcgtgccg tggaacttcc cgctgcacct gagcctgatc ccgctgacct
     4741 acgtcttcgc cgccggcaac cgggcgatgg tcaagatgag cgagaactcg cgccacctcg
     4801 cggccttgct gatcgagaag atgccggcct acttcccgcc ggagaagctg cagttcttcg
     4861 acgagaccgg cggcgtgggc atcgagttct cgaagctgaa gttcgaccac ctgctgttca
     4921 ccggctcggg cgcgaccgga cgctcggtga tggcggccgc cgcggccaac ctggtgccgg
     4981 tgacgctgga gctgggcggc aaggccccgg ccatcgtctg cgacgacttc ccgctgcgcc
     5041 tggcggccga gcgcatcctg ttcgcaaagc tgctcaacgc cgggcagatc tgcacgacgg
     5101 tcgaccacgc ctggctgccg cgcgcccgca tcgacgagtt cgtcgcgctc gcgggcgaga
     5161 tcgtgctgca gcgcttcggc tcgatggcct cggccgacta cacgtccatc atcgacgagc
     5221 gcgcgttcga gcgcctgctc gccgcgctcg aggacgcccg cgcccgcggc gcgcgactgg
     5281 tgcagctgct tcccggggag cccttcgacc gcgcgacgcg caagatcgcg cctcatctcg
     5341 tgctcgacgc accggccgac tgcgagctgc tgcagcgcga gatcttcggc cccatcctgc
     5401 cgctgcgcgc gtacgaccgc ctcgaggacg tcgtgcatgc catcaacgcc ggcgcgcggc
     5461 cgctcgcgat ctacccgttc agctacgacg ggcagcgcgt gcagatgctg ctcgaccggg
     5521 tgatgtccgg cggcgtgtcg gtcaacgagg cgctgttcca cgtcgcccag cacgacctgc
     5581 ccttcggagg cgtcggcgaa tccggcatgg gccactacca cggccgcgag ggcttcgaca
     5641 ccttctcgaa gctgcgtccg gtcttctacc aggcgcgcgt cagcggcatg aagttcatgg
     5701 cgccgcccta cggccgcttc gccaacgcgc tgctgggctt cctgaccaag tgaccacgcc
     5761 cggcgcgcgt cgccgcgggg tcacttcgac gccacgacgt tgggcaccag taccggcttg
     5821 tccatgtact gctcggggaa caggcgcttc aggtcggcga ccttgggcag gtcgttgtag
     5881 acgatgtagg ggtggtcggg gttcagcgcc aggaagttct ggtggtagcc ctcggccggg
     5941 aagaagccct tgaacggctc cacgcgcgtc acgaccggct tgccatacgc cttggcggca
     6001 tcgagctgcg cgatgtaggc cgtcgcgacc ttgcgctgcg cgtcgtccag ggggaagacg
     6061 gccgagcggt attgcgagcc gtggtccggg ccctggtagt tcagctcggt cgggttgtgg
     6121 gcgaccgaga agtagacctg cagcagcttg ccgtagctga cctgcgccgg gtcgaacgtg
     6181 acctgcacgg actcggcgtg gccggtcagc ccggtgccga ccttctcgta ctccgcggtg
     6241 gacttcgcgc cccccgagta gccggagacg gcgttcgtca cgcccttcac atgctggaac
     6301 acgccctgca cgccccagaa gcagccgccc gcgaagacgg ccgtctcggc atggcgggcg
     6361 gggttggcgg gctcgtcgac cgccgggggc gggatggcga ccgcggtctc cgcggcatgg
     6421 gccgtgccga tgaagtgcgt ggcggcgaag accaccgcgc ccgtcgcgag caaggcggcg
     6481 aagggacgca ggcggcgtga cggcgggagg gtggcggcgc gcgagaagcg ggtcatggcg
     6541 ggttccttgg caggtcgtcg ggacgtcagg cgggcgtgaa ggtcatcgcg acgccgttca
     6601 tgcagtagcg caggccggtg ggcttcgggc cgtcgtcgaa gacgtggccc aggtggccac
     6661 cgcagcggcg gcagtgcacc tcgtcgcgca ccatgcccag cgacaggtcg cgctcggtgg
     6721 ccacggcgtt gggcagcggc tgccagaagc tgggccagcc tgtgccgctg tcgaacttgg
     6781 tgtcggacga gaacagcgcc agcgcgcatc cggcgcacga gaaggtgccc ttgcggtgct
     6841 cgctgttgag ggggctggtg aacggcgtct ccgtcgcctc gtggcgcagc acggcgtagg
     6901 cccgcgggct cagcttggcc ttccactggg cgtcggtcat ctcgacctcg tagtgcgtgg
     6961 ccggcgcggc ggcgcgcgcg cggacgccgg cggcgacgag cgcgacgaga ccgccgccca
     7021 gcacgaactg ggcgccggac aggagagcgg tgcgacgggc gatgggcatg gctggctttc
     7081 cgagaaacga agtggcgacg tggatgggtc gggcgaccgg cgccgatcct tacacgcccg
     7141 gcgcggcact gggtgaatga cccggccgag ctcgaatggt tcatcgagcc tcccgcggcc
     7201 gggacgcgac agcgacgcgc gcggacgcgc ccgaccggcc ccgaatgccc gcagaatgcc
     7261 tgcatgagca cgtcccgcct cgacgacccc gccgtccgca ccgccgtcgc gcccaccggc
     7321 cgcctgcgcg cctcgatcaa cctcggcaac cccatcctcg cgaacgccga tcccgcgacc
     7381 ggccgcgccc gcggcgtctc ggtcgacctc gcccacgcgc tggccgcgcg cctggacatg
     7441 cccctcgaga cgatcgagtt caccgccgcc gcgaaatcgg tcgacgccgt ggccggcgag
     7501 gcggcggatg tcggattctt cgcgatcgac ccggcccgcg gcgcctcgat cgcgttcacc
     7561 gacgcctacg tgctgatcga gggcgcctat ctcgtgcgcg cggattcgac gctgcaggcc
     7621 aacgaggagg tcgatcgcgc cggcacgcgc atcgtcgtcg gccagggcag cgcgtacgac
     7681 ctgtacctca cgcgcgagat cggggccgcg cggttcgtgc gcgtgcccac cgggccggcg
     7741 atcgtggaga cgctggcggc cggaggcgcg gacgtggccg ggggcatccg ccaggcgctc
     7801 gccgacatcg cccgggagcg cccgggcctg cgcctgctgc ccggccgctt catggtgatc
     7861 cgccaggcga tgggcctgcc gcgcagccgc ggcgaggcgg ccgcggcggc gctgtcggcc
     7921 ttcgtcgagg agatgaaggc gagcggcttc gtcgccgacg ccctcgcccg ccacggcatc
     7981 tcgggcgcct ccgtcgcgcc gcccgccgac gcctgaaccc cttcccactc cgacaccgcc
     8041 tgcccatgga ttccccgaac gcgctgccgc gccaccagac ctcgatgacc gtgctcatga
     8101 cgcccgacat ggccaacttc tccggcaacg tccacggcgg caccatcctg aagctgctcg
     8161 atcaggtcgc ctacgcctgc gccgcgcgct acgccgcccg gtacgtggtg acgctgtcgg
     8221 tcgaccaggt cgtgttccgc gagccgatcc acgtcggtga gctggtgacc ttcctcgcct
     8281 gcgtgaacca caccggcacg agctcgatgg aagtcggcgt caaggtgctg gccgaggaga
     8341 tccgcaccca gcgcgtgcgc cacgccaaca gctgcttctt caccatggtc gccctggacg
     8401 acgatcgcaa gcccgtcgcg gtgccgccgc tcgtgccggc gacccccgag gagcgccgcc
     8461 gccacgccgc cgccgagctc cgcaaggtgc tgcgcgccga gtaccgcagc cggttcgagg
     8521 ccgtccgcca ggccaatccg gacgcggcct gagcgacccg ctggccccgg cgccgccggc
     8581 agccttggcg tcatcagtga ttttgcaaat gcgaatgagt cgcattaaac tgatggcatg
     8641 ccttgctcga tcggcttgcc ccctgcacca cgcctgccac cccgctcgcg gaggccgtcg
     8701 tgaaccgccg cccgcccctg ccgcggcgcc gccgcctgcg ccagggcctg ccgcgtcggc
     8761 gaccgtcccg cgagcgcccg gcgctgcctc ccctcgccgt tccggcccgc gcgcccgaca
     8821 cgtccgccga tccgcgcgcg gacgagcgcc tggccctcgc cgcgctgagc ctggagaa
//
LOCUS       DASVLN010000142         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_276496, whole genome shotgun sequence.
ACCESSION   DASVLN010000142 DASVLN010000000
VERSION     DASVLN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276496"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..>2150
                     /gene="kefC"
                     /locus_tag="VF453_03145"
     CDS             266..>2150
                     /gene="kefC"
                     /locus_tag="VF453_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HEX7686672.1"
                     /translation="MNHGHWLTLALTYLAAAVIAVPLARRLGVGAIIGYLGAGFAIGP
                     WGLRLVTDPSQVADLAEFGVVLMLFLIGLELEPKRLWAMRRPIFGWGSAQLLGSMAAI
                     GAIGWACLGHGADALPVAVVAGAALAMSSTAIGLATLAERNILQTTVGQSVLSVSLFQ
                     DIAAIPLLALIPLLAPDRVAGEDAGHPWLAAGRAFGMIAAIVLGGRLAIRPALRWIAR
                     SATPEIFTAAALGLVVGTAALMRAVGLSEALGAFLAGVLLAESEYRRELETDIEPFKG
                     LLLGLFFIAVGMGIDAGVVASHPWPVAEIVVGFLVVKALVLWGIELGIPIPRPERPVF
                     TLLLAQGGEFGFVVIQAAVGKDVIDAATAGVLTAAITVSMLLTPLLMLAADRWWIPLV
                     QKYHPRKRLSELAEPQDAPVIIAGFGRYGQIIGRLLHANGIEMTVLDHDAEQVDALRR
                     FGFRVHYGDATRLDLLRVAGAARARVIVVAVDGMDASLELVDLVKEHFPNLVIVARAR
                     NVQHWYQLNERGVAFIERETLDAALMSARSVLETMGLEPYRARQLAMRFRRHSVAQLH
                     ALAPHFRDESKLVSLARAGRQQLEQLFAQERHDRRPAAPTWDVGAQPGAEFDAHAPAP
                     PRDE"
BASE COUNT          245 a          829 c          774 g          302 t
ORIGIN      
        1 gctcgctccg ctccccgccc ccagggggct cacgcgggct cgctccggag cggccgagcg
       61 ctcgccggcg gacaccacga cccggctcgc tccgctcccc gcccccaggg gcctcacgcc
      121 ggctcgctcc ggagcggccg agcgctcgcc ggcggacacc acgacccggc tcgctccgct
      181 ccccgccccc agggggctca cgccggctcg ctccggagcg gccgagcgcc cgccggccgg
      241 acaccgcaac gactgacccg ccaagatgaa ccacggccac tggctcaccc tcgccctcac
      301 ctacctcgcc gccgcggtga tcgcggtgcc gctcgcgcgc cggctcggcg tgggcgcgat
      361 catcggctac ctcggtgcgg gcttcgcgat cgggccctgg ggcctgcggc tggtgaccga
      421 tccgtcgcag gtggccgacc tggccgagtt cggcgtcgtg ctgatgctgt tcctgatcgg
      481 gctggagctc gagcccaagc gtctctgggc gatgcggcgg ccgatcttcg gctggggctc
      541 ggcgcagctg ctgggcagca tggcggccat cggcgcgatc ggctgggcct gcctcggcca
      601 cggcgccgac gcgctgcccg tcgccgtcgt cgcgggcgcg gcgctcgcga tgtcgtcgac
      661 cgccatcggc ctggcgacgc tcgccgagcg caacatcctg cagacgaccg tcggccagtc
      721 ggtgctgagc gtctcgctgt tccaggacat cgccgcgatc ccgttgctgg cgctgatccc
      781 gctgctcgcg ccggaccgcg tcgccggcga ggacgccggc cacccgtggc tcgccgcggg
      841 ccgcgcgttc gggatgatcg ccgcgatcgt gctgggcgga cggctggcga tccggccggc
      901 gctgcgctgg atcgcccgca gcgccacgcc ggagatcttc accgccgcgg cgctcggcct
      961 ggtcgtgggc accgcggcac tgatgcgggc ggtcgggctg tcggaggcgc tcggcgcctt
     1021 cctcgccggc gtgctgctgg ccgagagcga gtaccggcgc gagctcgaga ccgacatcga
     1081 gccgttcaag ggcctgctgc tggggctgtt cttcatcgcc gtgggcatgg gcatcgacgc
     1141 cggcgtcgtg gcgagccacc catggccggt ggcggagatc gtcgtcggct tcctcgtggt
     1201 gaaggcgctg gtgctgtggg gcatcgagct gggcatcccg atcccgcggc cggagcggcc
     1261 cgtcttcacg ctgctgctgg cccagggcgg cgagttcggc ttcgtcgtga tccaggcggc
     1321 ggtcggcaag gacgtgatcg acgccgccac cgcgggcgtg ctgaccgcgg cgatcaccgt
     1381 gtcgatgctg ctgacgccgc tgctgatgct cgcggccgac cgctggtgga tcccgctcgt
     1441 gcagaagtac cacccgcgca agcggctgtc ggagctcgcc gagccgcagg acgcgccggt
     1501 gatcatcgcc ggcttcggcc gctacggcca gatcatcggc cgcctgctcc acgccaacgg
     1561 catcgagatg accgtcctcg accacgacgc cgagcaggtc gacgcgctgc gccgcttcgg
     1621 cttccgcgta cactacggcg acgcgacgcg gctggacctg ctgcgcgtgg cgggcgcggc
     1681 gcgggcgcgc gtcatcgtcg tggcggtcga cggcatggac gcgagcctgg agctggtcga
     1741 cctcgtgaag gagcacttcc ccaacctcgt catcgtcgcc cgcgcgcgca acgtgcagca
     1801 ctggtaccag ctcaacgagc gcggcgtcgc gttcatcgag cgcgagacgc tggacgccgc
     1861 gctcatgagc gcccgcagcg tgctcgagac catgggcctg gagccgtacc gcgcgcgcca
     1921 gctcgcgatg cgcttccgcc gccactcggt cgcgcagctg cacgcgctcg ccccgcactt
     1981 ccgggacgaa tccaagctgg tctcgctcgc gcgcgccggc cgccagcagc tcgagcagct
     2041 gttcgcgcag gagcgccacg atcgccgccc cgcggcgccg acctgggacg tcggcgccca
     2101 gcccggcgcc gaattcgacg cccacgcccc cgcgccgccg cgcgacgagg
//
LOCUS       DASVLN010000143        27238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_437510, whole genome shotgun sequence.
ACCESSION   DASVLN010000143 DASVLN010000000
VERSION     DASVLN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 27238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27238
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437510"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1242
                     /locus_tag="VF453_03150"
     CDS             43..1242
                     /locus_tag="VF453_03150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7686673.1"
                     /translation="MKVIKLREGKERSLLRRHPWVFQGSIDKGRADAGETVRVEGHDG
                     RFLAWGAYSPESQIRVRAWSFDQAERIDAAFFARRLETAIALRARMGIPSDGVRLVHG
                     EADGLPGLVVDRYGELLSAQFLAAGVERWKDAIADALLRQTGCSRLYERSDASVRALE
                     GLPSVRAWLRGGSDDPAASRTTIRESGWKLDVDVAEGHKTGYYLDQRDNRARFAQLVR
                     QYGCKRVLNCFSYTGGFSVAALAGGAERVVSVDSSAPALALADAHVRLNGFDPARHES
                     RDSDVNALLRAALQSGERFDAIVLDPPKFAPTAAHADRASRAYKDINRLALKLLEPGG
                     LLLTFSCSGGISADLFHKIVAGAAMDAEVDGALIARLEGAPDHPTTLTFPEGEYLKGL
                     AILKSAA"
     gene            complement(1276..1776)
                     /locus_tag="VF453_03155"
     CDS             complement(1276..1776)
                     /locus_tag="VF453_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7686674.1"
                     /translation="MDALDEINDLSRSSGELDAVDRRLLELLQADASRTNQALAEAAG
                     VSPATALRRVRRLVVSGVIERQVALLAPRFADAGLSAIAEITLDRQGAELLDAFEARA
                     VAEPAVQQCYRVSAGPDFVLVAWVPDMAAWSALVQRLFTQDANVRNVKTYFSVKRAKF
                     DPRVPV"
     gene            1906..3783
                     /gene="glmS"
                     /locus_tag="VF453_03160"
     CDS             1906..3783
                     /gene="glmS"
                     /locus_tag="VF453_03160"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX7686675.1"
                     /translation="MCGIVGGVSTRNIVPILVEGLKRLEYRGYDSCGVAVHQDGALRR
                     TRSTARVAELAEQIAADHVAAGTGIAHTRWATHGAPAVHNAHPHFSGVLPPGAATSAA
                     DASRGARIALVHNGIIENHDELRAELKARGFVFESQTDTEVIAHLVNHLYDGDLFDAV
                     QRAVTRLKGAYAIAVFCRDEPHRVVGARAGSPLVLGLGEGENFLASDAMALAGVTDQI
                     VYLEEGDVVDVQLGKHWIVGLQPDGRHRAVEREVRTVHAHTGAAELGPYRHYMQKEIF
                     EQPRAIGDTLDGINGISPELFGDGAWRAFKDVDQVLILACGTSFYAGSVARYWLESIA
                     RIPTSIEVASEYRYRDSVPNPRTLVVTISQSGETADTLSALRHARALGMDKTLTICNV
                     STSAMVRECRFAFVTRAGAEIGVASTKAFTTQLVGLFLLTLALAQVRGHLDDATEDAH
                     LKALRHLPAAVQAVLALEPQIMAWSDEFAAKENALFLGRGLHYPIALEGALKLKEISY
                     IHAEAYPAGELKHGPLALVTAAMPVVTVAPHDALLEKLKSNMQEVRARGGELFVFADT
                     DTRIDSDTGLHVIRMPEHYGVLSPILHVVTLQLLAYHTACARGTDVDKPRNLAKSVTV
                     E"
     gene            complement(4056..4499)
                     /locus_tag="VF453_03165"
     CDS             complement(4056..4499)
                     /locus_tag="VF453_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686676.1"
                     /translation="MTPEQYRVWLGTQFMLLSDVAPGQDSLFDQIVESLFTNPQIVVL
                     VWIGKYTSEKCLLSDRGFSNSINLPYVDSIDFNLRHDAFIRYVFRPRRMVLQRVPAHV
                     IDAMPKLFNVYYSVDDMDHLRTYNWNVMNQSRSHVFCASGTGVVH"
     gene            complement(4525..4938)
                     /locus_tag="VF453_03170"
     CDS             complement(4525..4938)
                     /locus_tag="VF453_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686677.1"
                     /translation="MITLVNSGGWVFPATIASVVAREPQHARIHVNLALDDVFSFDVK
                     QNSTLRENFEALFHRYEDSVQEHTEAVLRKAAANDNDGMHFEVFEVFKAKLMNFMRNP
                     FAVAKMLDTFRNVSTLVPTNAERRAELEPVLEQAA"
     gene            complement(4935..5174)
                     /locus_tag="VF453_03175"
     CDS             complement(4935..5174)
                     /locus_tag="VF453_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7686678.1"
                     /translation="MDFREGNLRHARNLTQAELASRCGLDRSYIGSIEQARRNISIDN
                     IDRLAEALGVQARDLLARIVLPGEASDRHRPPGSE"
     gene            5214..5786
                     /locus_tag="VF453_03180"
     CDS             5214..5786
                     /locus_tag="VF453_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX7686679.1"
                     /translation="MDEIADYSAAIARARTLDWSHYPYTAIVVPGIGPDDLDVSLSAR
                     GKLNVRLAAARFAQGLAPFTLVSGGRVHPRGTHYAEALEMRRALMERFGIPGDCIVVD
                     PYARHTTTNLCNAVRRLTALGAPLDRDALIVSNVEQIQYMVSADFASRNARELGYQPG
                     LVKPRTSANELEFMPSAMSLRGDPADPLDP"
     gene            6077..6304
                     /locus_tag="VF453_03185"
     CDS             6077..6304
                     /locus_tag="VF453_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686680.1"
                     /translation="MLPLDFPIADSCLHLIEGTAATAPLYMDGYEALVLRTARVEGFF
                     VFGVRNVSIPFAAPAMTGHVLASLSREDAPP"
     gene            6658..7662
                     /locus_tag="VF453_03190"
     CDS             6658..7662
                     /locus_tag="VF453_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7686681.1"
                     /translation="MNKHTLIHACAGIAAVTALSTASAADAPAVSLTGDIKAAATYGN
                     GGQSPVDGAAADHVALNDNSSYFSLSARQDLPGGLYAGVQLDSFFGIDTGSDATNGNF
                     FSRRAVGKLGGSFGEIYFGRSLTPASLMVLFTDPWYWDGSVAQAGWMVQLANYTSTQY
                     LRTNNTIGWVSPKMSGFTLSLAASAGEKVNSKDLGGSLTYDNGPLSAGIAFDQSHGFF
                     NDPTLNRTIVGVASYDFGVVKPMLSFTSSKVAGVNYSSVVVAATAPAGQSGLLKAQYS
                     HMNDVDTATAGRQAYDKFALGYQYNLTKSVNFFGQVSDAKEKSLSATNTLELGMEYGF
                     "
     gene            8037..9218
                     /locus_tag="VF453_03195"
     CDS             8037..9218
                     /locus_tag="VF453_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HEX7686682.1"
                     /translation="MIVIAVAVAVFVFFDWTAWRGGPSPQGPSSVAPPPVVIGVAVGL
                     TGANGVVAPTVVQSSQLAVDEINRAGGILGRRVDLVVADDASDPAGVPAAAQQLLQQH
                     HADVVIAMETSAARTAMLPFIERARVPYVYTSFYEGHACSPWMYANGWVPEQQVVPVV
                     AYLMHDKAARSVYLVGSDYAFGRGMLEFTRRYIESHGGRVAGEDYLPVADTDWSTIVA
                     RIRAAKPDALISATAGGAPNVALAKQLDSAGLHLPYGNLAIDESTARTMGDRATGMLM
                     SASYLTSIDSTENHDFLAHMLIRFGDKLLAPNELSVPQYEAIHLYRLAAERAGTTESH
                     AVLAALGAVTFDGPRGLVQMSASRHAALAMRLARVQADGSARILQTFGNTDPGAQCPG
                     F"
     gene            9139..10863
                     /locus_tag="VF453_03200"
     CDS             9139..10863
                     /locus_tag="VF453_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7686683.1"
                     /translation="MCRQTARLASCRPSATRTPARNAPASDDPPSPTGEIDMTWNNRF
                     SRIGARLALGFGGLLALMCVIAATSGWSLLATGAQVRQIVEVNDRRVSQARDQLDAID
                     AMTAAVRSIALFTDMDALKSENAKLEAAQRRYDEAAHALEASLADAPDDERALAAEIA
                     RLGRDGESMVRSIAKQGLAGANIDVTTALTQTLPPVEATWRAKVGALVELLSRRNAEM
                     STAATQRTHDATLACGALAVIATVVGGFFAWVIARGIQRPLADAVRVAERIAAGVLDS
                     PIESGGAGGASEIRRLLGAVGAMQARLRDLVSAILEAAGSLQVASAEVAAGNADLSRR
                     TEHVAARLQEAASAVERLSTDVRHGVDASAKASELAREAAQIAATGGEVVDKVVSTMT
                     RISTSSQRMADIIGVIDGIAFQTNVLALNAAVEAARAGDLGRGFAVVAGEVRLLAGRS
                     ASAAKEIGELIEASMRHIDEGATLVRDAGGAMGRIVVGARHVSDIIGEVSTAARHQSA
                     RVDEISVAASEVDQATQQNAALVEQSAAAADSLRDQADRLAQLVSAFELGDRVAPARD
                     GVDAVVAN"
     gene            11050..11946
                     /locus_tag="VF453_03205"
     CDS             11050..11946
                     /locus_tag="VF453_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase family protein"
                     /protein_id="HEX7686684.1"
                     /translation="MFPSGTPGERSAFPDIVVACVQMAPQVGRKHDNVGRGLAAIERA
                     ADQGAQLVVLPELCNTGYVFDSPEEAFSLAEDVRDGQTVQAWMDAARRRDLVLVAGLA
                     ERDGDRLYNSAAVIGPGGLIGHYRKLHLWNEEHLFFEPGDLGLPVFDTPLGRIAPLIC
                     YDGWFPETYRYLAQRGVDIVCMPTNWVPMPGQSDDERAMANTLAIANAHVSAVNIACA
                     DRVGTERGQRFIGRSLIVAAGGQVLAGPASADDEELLLARLNLASSRQASRFNAFNDA
                     LHDRRGDVYGTVHGDDWPPQCS"
     gene            11979..13145
                     /locus_tag="VF453_03210"
     CDS             11979..13145
                     /locus_tag="VF453_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HEX7686685.1"
                     /translation="MNKSFRPVLNFGATAALAVLALGARAEEPIKIGVAVGLSGANSV
                     VAPAVVQSSQLAVDEINAAGGILGRKVQLEIVDDASGAVGAQKAYDTLVFQKKVDAVI
                     SMETSAARNAALPAITRGKVPFIYTSFYEGHSCNPWMYVNAWVPEQQVAPIVNYFWST
                     LKARNYFLVGNDYAFGRGMLEFTRKYIEKNGGKVVGEEYLPIDGSDWTSVISKIREVH
                     PDALVSATAGGAPNVALAKQLQGSGLAIPYGNLAIDEGTAHNMGDAAKDMYMSASYLT
                     NIDTAGNHHFVDALNKKFGGELKTPNELSEPQYEAFFLYKAAVEKAKSTDHAAVVKAL
                     GEVSFDGPRGDVRMNRSRHAPLAMRLGQVQADGSIHILQTFKSVDPGEQCPLLK"
     gene            13208..14041
                     /locus_tag="VF453_03215"
     CDS             13208..14041
                     /locus_tag="VF453_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7686686.1"
                     /translation="MALLLDIITTAAVLFIVTSGLMVIFGVMKIVNFAHGALLTLGAY
                     ASLVVTRLALPPWLGMPLALVVGTAAGALVERFVIRPLYKRPLDAILATWGLGIVIGQ
                     VIVAVFGREVQFVQAPLSGAVAIAGTTYSAYRLVLVPIALVLCGLLLALLAGTRFGLK
                     TRAVIMNEDLAESLGIHAGRVRFATFALGAALGTLAGCLITPLSSVDPGMGLTWLVSA
                     FMLVMVSGHSMASLLLTCLVFGACQVLVSAYANPVLGGMTIALLAALTLRIRPAGFAR
                     G"
     gene            14049..15842
                     /locus_tag="VF453_03220"
     CDS             14049..15842
                     /locus_tag="VF453_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX7686687.1"
                     /translation="MIIPKIQSPATRSRAARPLTFVLAVGAIVLAAGPWLFDDYLLNV
                     LVKAFFFAIAAVTVDLLWGYMGHLTFGQSAFFGLGAYASALVFTHHGLSWGTAVIALG
                     LAVVVPAGVAALLGWLSFYRGASPFFATVMSLVLPIVASQLLLSGGEWTGSSSGLTGY
                     ETPDLPLGAWYAIAGLLLAGAGALGWMVVRGDAGRVLVAIRDNELRCAYLGLRVHAIK
                     IVLLVATAVVAGVAGFGYGAFSGVVAPELTGFVFGTELIIWVVLGGRATLWGPLAGAV
                     LINVATSYLSSSLPFAWPLVVGVVFVVVIVLLPQGVVPLAVRLVDRGRSPVSPAPVLR
                     EAAPAAKPASDGPAFEMRDVAKRFGSLAVLQGIDLRADAGDLVGLIGPNGAGKTTLMR
                     CMSDGSERSAGDVRLSGRDIRNAAPDDCVRLGLGRKFQNANIFESLTVAECLRMARTI
                     VQAPRLLGRAPTLELPSYALEVMRVTGLEAKLAVVARDLSHGEQQALELAMVLALEPR
                     IVLLDEPTAGLTKTERRQIGEVLSALALRHGRCCLLVEHDLDFVEEIATRIVVMHQGR
                     IVMEGPFREVVESELVKTIYAGSAAPAREVA"
     gene            15839..16546
                     /locus_tag="VF453_03225"
     CDS             15839..16546
                     /locus_tag="VF453_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7686688.1"
                     /translation="MMARALDIEGVSSGYKASLVLRNASLTVAKGEAVALLGKNGMGK
                     STLMKTVMGYLPKQSGRVHVGSDDVTRASPHQVAACGVAYAAQERPLFNDLSVRDNLR
                     LGLAQERLFDERFAEIEPLFPVFKSRLRQPAGTLSGGEQKMLLVARSLMMRPALMLLD
                     EITEGLQPSVIDRIAHALRWERERHGTSMLLVEQHVAFALAVVDRYVVLKRGEFIDSG
                     DARAQGARDAVFDHLQV"
     gene            16518..17558
                     /locus_tag="VF453_03230"
     CDS             16518..17558
                     /locus_tag="VF453_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX7686689.1"
                     /translation="MLSSIISRSRPMTLFAAATEGLPRCVVIATGGTVAMRPDPRSGA
                     PVPALSGEELVASVPQLLGLARLDVRSVFNVPSVEMGPARWLALRQALLAALDDDEVC
                     GIVVTHGTDLLEETAWFLALTLPAECPVVLTGAMRHAAESQFDGPRNLLDAVRVAISP
                     SARGMGVLVVMDGEVYCAREAEKSHTTETSAFRRGPGIELGAIGAEGPAFWRKPRPHP
                     RIEPAGGDMPRVDIVPMFGGADGLPIRWAVQSGARGVVVQAVGAGNVSAAMYTELLNA
                     LEAGVMVVVATRVRGGRSQPVYGFLGGGTLLAQAGAIFSGGLSPQKARILLMLALQPR
                     WSTAQVRELFDF"
     gene            17587..18735
                     /locus_tag="VF453_03235"
     CDS             17587..18735
                     /locus_tag="VF453_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011494388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7686690.1"
                     /translation="MSREDASTSNAGWRVGILYSRSGATSVTESEHFFGTTLAVEEIN
                     AAGGVLGRPLLPVAYDPKGDSGEYRRLANRLVIDDDVNVIFGCSRSSSRKAVLPVVER
                     NNALLWYCSFYEGFEYSPNIVYTGAVPNQNSVQLAAYLLQHCGTRLFLVGADYIYPRE
                     SNRVMRDVVERHGGEILDEVYLPIEAERAELEEVLDDIEKAQPSVVFSTLVGQSGRLF
                     YTMFHERGLDANRMPIASLGMAEEEIRLVGPERCVGHITAATYFSSLQSSANDHFKKL
                     WHDRFGERPTSTWSEMAYSQVHLFARALERSGSLDTVKLVNALRKVRFDSPEGPIAID
                     VDNNHLALTPRIGRCRADGQFDVVWQSAEPIRPDPYLSTFGFANFWLK"
     gene            18740..19354
                     /locus_tag="VF453_03240"
     CDS             18740..19354
                     /locus_tag="VF453_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX7686691.1"
                     /translation="MSSVRRLYEDLRSISVVVIHPPGEDRDVIVQQLQRIGCRLRTVW
                     PFPAEPPSQVDVVFFQVSQDLADHGGWSASALDSAEVTLVALSDYENPTTLKLLLDSH
                     AHGVITKPFRSSGILSTLVLARSAHSFQQRMQGKIDKLETTMKSRRVMEKAIRLLVDH
                     QKMSEVQAYEHMRSRATSLRVTVAEVAGMMIDAQEAMEKLGLRG"
     gene            19478..20634
                     /locus_tag="VF453_03245"
     CDS             join(19478..19745,19745..20634)
                     /locus_tag="VF453_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611359.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX7686692.1"
                     /translation="MSKPRRHFSDEFKREAVRMCLEPGAVILQVARNLGIDQSVLRRW
                     VQAERGGRLEMNSAKPLRSEAATELERVQRELRRVTMERDILKKAPRLLREGPAVKYA
                     WIAKQRGTWSTRAMCRLLEVSHSGFYEWLGRGPSARSLEDARLTRGIRQSFELSDRTY
                     GSPRVWHDLRAAGERCGRHRVARLMNAANLKARTRRRRMPGDGAQRLEHNIAENRLQR
                     EFEADGPNKKWVADFTYVWTAEGWLYVAAVMDLYSRRIVGWSMSESMHAKMVTDALLM
                     ALWRRGRPSQLLLHSDQGSQYTSEEFQSMLRAEGITCSMSRRGDCWDNAAMESFFSSL
                     KTERLSRKVYRTRDELRADVFDYVERFYNQQRRHSTLDFVSPVQFEQGLMG"
     gene            complement(20743..21249)
                     /locus_tag="VF453_03250"
     CDS             complement(20743..21249)
                     /locus_tag="VF453_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686693.1"
                     /translation="MATLHLELEIDSDVHPELYARLASIERSAARQERLRQLAATGLI
                     WEIARLHGPAFSVPEAVDPADPPPPPVDLGGDPASAVDAGSVIDHAPVVPQDVPVLVD
                     VVDEAEMNGAAIVDPPARALAAPAREGPDPRGSAPFASPSPVEAPHGRTRSARIRRMK
                     ESGLFQNG"
     gene            complement(21260..22321)
                     /locus_tag="VF453_03255"
     CDS             complement(21260..22321)
                     /locus_tag="VF453_03255"
                     /inference="COORDINATES: protein motif:HMM:NF037161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686694.1"
                     /translation="MVRAIDVGFGLVKFSLPAAGGDVGFRSFPSLAIPADTSAVRSLS
                     TRRRDTFDVPVQGALYEVGQDVGLALAGGSFGRDVTDEFYKGKVYEAVTKGALRYMAE
                     AGDSTIDVLVLGLPVHQFNDTARRSYLRRTYEGPLDVGGGKTVTVARVEVQAQPMGGY
                     AALEEHLDELNREIAATGGALQPLASVDDLDQLAVLMVDPGEHTLDWLLIQQGTINPS
                     ASNAASDAGRHRVLKAVQAALSEDVGRPLGVSVQPHINEALRKKTPVKLSGVAYDLSR
                     YEPRVMSVIEDAVNRMIDGLRDATEIVDLIVLVGGHPDRYRDVLKQRFPAIPVFVMPE
                     SVTANVRGFQMIGEAVAES"
     gene            complement(22911..23396)
                     /locus_tag="VF453_03260"
     CDS             complement(22911..23396)
                     /locus_tag="VF453_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686695.1"
                     /translation="MNNHAVAIAAALIACAAPCCDAGTKEKAMSSKPEPTASRAPPQR
                     VPPVVIDGVRYAPVIGDIDRDGQIGGILGAFDASNRELWRLRVYENVRMPGVEGDVQD
                     VYFRSMRADGRKLLIENEVGERFEVDTVSRRVVAHLPAAPRSNIDPISGRQRMPPPPE
                     D"
     gene            complement(23393..24544)
                     /locus_tag="VF453_03265"
     CDS             complement(23393..24544)
                     /locus_tag="VF453_03265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF025874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HEX7686696.1"
                     /translation="MANPVPYLLIHRKLVAATQTPDKYANDWKVIAEALPNLVRDDGF
                     NYLQGGIPERIREKLAGAKSQSKAIRTAAKAEGSLVSNPSDNACARALLLKTLSHLYF
                     YESGGERKLWILSLPATYVAHPIQYAKSPDAVVDQLLEQNVEIFTDEQKRRIESAVSM
                     SLRWVERAMIVAGDPTRPEHMTLLKRWFVPATHPDADGAIAAFTPTLRRGLLKIAIGL
                     KVGDLIILDDPGQRGTNSKWEQSEAYTFPRDDIHAVWVEPGFWGGGNTLTGATNWARI
                     VVHELTHNYCQTEDHSYSWQGLLPRESDVFRRVNDARVAQDAGFSAVRTLTMEQCLTN
                     ADSWAFFCADAAGALTDRDRIAALGSKLYADTGWRPTQKVVRTVVAQAR"
     gene            complement(24687..25406)
                     /locus_tag="VF453_03270"
     CDS             complement(24687..25406)
                     /locus_tag="VF453_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7686697.1"
                     /translation="MGAQWKAKGKELAANAKGKLFGKLAKDLMLAAKAGADPAGNAKL
                     RLVIEQARKVSMPKDTLERAIKKGAGLTGETVNFEHVFYEGFAPHRVPVVVECLTDNV
                     NRTAPEIRVIFRKGQLGAPGSVTWDFDHVGMIEAEPATPGVDPEEAAIEAGAQEVEPG
                     DEDGTTLFLTDPTDLDLVSRALPEHGFKVLSAKLGYKAKNPVDPASLSPEALEEVETF
                     LAALDEHDDVQNVFAGLGSAG"
     gene            25601..25765
                     /locus_tag="VF453_03275"
     CDS             25601..25765
                     /locus_tag="VF453_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HEX7686698.1"
                     /translation="MFLVLKCLLGAAAVLLIALLSRSRNFHLAGLVPLFPTFALIAHA
                     AGRMAEMKPS"
     gene            complement(25762..26181)
                     /locus_tag="VF453_03280"
     CDS             complement(25762..26181)
                     /locus_tag="VF453_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7686699.1"
                     /translation="MTQPRAGAGGGQVAGGCLCGDVRVVASGRPYRVGLCHCLDCRRH
                     HGALFHASAIFPREAVAIEGETREFAGRSFCPRCGSSVFSRSGDEVEVSLGALDAPDL
                     FAPTYELWTIRRESWLPPFPLAHRYERDRDASSRFED"
     gene            complement(26178..27074)
                     /locus_tag="VF453_03285"
     CDS             complement(26178..27074)
                     /locus_tag="VF453_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017332912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HEX7686700.1"
                     /translation="MDQRTQVIAVPDEERDAAAPSPRLADALERGLVLHMPAFPFPLS
                     ADELGVLDESNVDPDRKNISFDPKTGGLAGFVGPQDARRTAEGLVRRYHETAQALIDR
                     LFPHYRGRMRAAPASLRLHPIEGKALSWRKDDSRLHVDAFPSRPNRGERILRVFLNIN
                     PHGVARVWRVGEPFEDIARRFLPQLRPPLPGSAWLLNALHVTKSPRSRYDHLMLGLHD
                     AMKADMGYQANSPQEEVAFPPGSAWICFSDQTSHAAMSGQFMLEQTFHLPLAAMADPA
                     KSPLKVLERLVGRRLVEGEASR"
BASE COUNT         4302 a         9251 c         9227 g         4458 t
ORIGIN      
        1 cgcgcccgcc gcccgcgcgc gcggggcggc gacaatcgcg ccatgaaagt catcaagctg
       61 cgcgaaggca aggagcgctc gctgctgcgg cgccacccgt gggtgttcca gggcagcatc
      121 gacaagggcc gcgccgatgc cggcgagacg gtgcgcgtgg agggccacga cggccgcttc
      181 ctcgcatggg gcgcgtacag ccccgagtcc cagatccgcg tgcgcgcctg gagcttcgac
      241 caggccgagc gcatcgacgc cgcgttcttc gcgcgccggc tcgagaccgc gatcgcgctg
      301 cgtgcgcgca tgggaatccc cagcgacggc gtgcgcctcg tccacggcga ggccgacggg
      361 ctgcccgggc tggtcgtgga tcgctacggc gagctgctgt ccgcgcagtt cctcgccgcc
      421 ggcgtcgagc gctggaagga cgccatcgcc gacgccctgc tgcgacagac cggctgcagc
      481 cgcctgtacg agcgcagcga cgccagcgtg cgcgcgctcg agggcctgcc gtcggtgcgc
      541 gcctggctgc gcggcggcag cgacgacccg gccgcctcgc gcacgacgat ccgcgagagc
      601 ggctggaagc tggacgtcga cgtcgcggag ggccacaaga ccggctacta cctcgaccag
      661 cgcgacaacc gcgcccgctt cgcccagctc gttcgccagt acggctgcaa gcgggtgctg
      721 aactgcttca gctacaccgg cggcttctcg gtggcggcgc tggccggcgg ggccgagcgc
      781 gtcgtcagcg tcgactcgtc ggcgccggcg ctcgcgctgg ccgacgccca cgtgcgcctg
      841 aacggcttcg acccggcgcg ccacgagagc cgcgactccg acgtcaacgc cctgctgcgc
      901 gcggcgctcc agtcgggcga gcgcttcgac gcgatcgtcc tcgacccgcc gaagttcgcg
      961 cccaccgcgg cccacgccga ccgcgcctcg cgcgcctaca aggacatcaa ccgcctcgcg
     1021 ctcaagctgc tcgagcccgg cggcctgctg ctgacgttct cgtgctcggg cggcatcagc
     1081 gccgacctgt tccacaagat cgtcgccggc gccgcgatgg atgccgaggt cgacggcgcg
     1141 ctgatcgccc ggctcgaggg cgcgcccgac catccgacca cgctcacctt ccccgagggc
     1201 gagtacctca agggactcgc gatcctgaag tcggccgcgt gacggcgcgg cgcggacgcc
     1261 gctcccgtcc ggccgtcaga ccggcacgcg aggatcgaac ttggcgcgct tcacgctgaa
     1321 gtaggtcttc acgttgcgga cgttggcgtc ctgcgtgaac aggcgttgca cgagggcgct
     1381 ccaggccgcc atgtccggca cccaggccac cagcacgaag tccggccccg ccgagacgcg
     1441 gtagcactgc tgcaccgccg gctcggccac cgcgcgcgcc tcgaaggcgt cgagcagctc
     1501 cgcgccctgg cggtcgagcg tgatctccgc gatcgcgctc aggccggcgt cggcgaagcg
     1561 cggcgccagc agcgcgacct ggcgctcgat caccccggac accaccagcc ggcgcacgcg
     1621 ccgcagggcc gtcgcgggcg agacgccggc cgcctccgcc agtgcctggt tggtgcggct
     1681 ggcgtcggct tgaagcagct ccagcaggcg gcggtcgacc gcgtcgagct cgccgctcga
     1741 cctggaaaga tcatttattt catccagcgc atccatatgg cattttcatt ctaccctgga
     1801 accgactgaa attttcggtc aatccaatgc gaacttagaa aacatccatc tctccgaccg
     1861 gcctacgatt catccatcgt catgttcgaa tccggagacc cacccatgtg cggcatcgtc
     1921 ggcggggtca gcacccgcaa catcgtcccc atcctcgtcg aaggcctgaa gcggctcgag
     1981 taccgcggct acgactcctg cggcgtcgcg gtgcaccagg acggcgccct ccggcgcacg
     2041 cgcagcaccg cgcgcgtcgc ggagctcgcc gagcagatcg ccgccgacca cgtcgccgcc
     2101 ggcaccggca tcgcccacac gcgctgggcc acgcacggcg cgccggccgt gcacaacgcg
     2161 cacccgcact tctcgggcgt cctgccgccc ggggcggcca ccagcgcggc cgacgcctcg
     2221 cgcggcgcgc gcatcgcgct cgtccacaac ggcatcatcg agaaccacga cgagctgcgc
     2281 gccgagctga aggcgcgcgg cttcgtcttc gagagccaga ccgacaccga ggtgatcgcc
     2341 catctcgtga accacctgta cgacggcgac ctgttcgacg ccgtgcagcg cgccgtcacg
     2401 cgcctgaagg gcgcctacgc gatcgcggtg ttctgccgcg acgagccgca ccgtgtcgtc
     2461 ggcgcgcgcg ccggctcccc gctggtgctc ggcctgggcg agggcgagaa cttcctcgcg
     2521 tccgacgcga tggcgctggc gggcgtcacc gaccagatcg tctacctcga ggaaggcgac
     2581 gtcgtcgacg tgcagctggg caagcactgg atcgtcggcc tgcagcccga cggccgccac
     2641 cgcgccgtcg agcgcgaggt gcgcaccgtg catgcgcaca ccggagccgc ggagctcggg
     2701 ccctaccgcc actacatgca gaaggagatc ttcgagcagc cgcgcgccat cggcgatacc
     2761 ctggacggta tcaacggcat cagcccggag ctgttcgggg acggcgcgtg gcgggcgttc
     2821 aaggacgtcg accaggtgct catcctcgcc tgcggcacca gcttctacgc cggcagcgtc
     2881 gcgcgctact ggctcgagag catcgcgagg atccccacca gcatcgaggt cgcgagcgag
     2941 tatcgctacc gcgacagcgt cccgaatccg cgcacgctgg tcgtcaccat cagccagagc
     3001 ggcgagacgg ccgacacgct ctccgccctg cgtcacgcgc gcgcgttggg catggacaag
     3061 acgctgacca tctgcaacgt gtcgaccagc gccatggtgc gcgaatgccg cttcgcgttc
     3121 gtcacgcgcg ccggcgcgga gatcggcgtc gcgtccacca aggccttcac gacgcagctg
     3181 gtcgggctgt tcctgctcac gttggcgctc gcgcaggtgc gcggccacct cgacgacgcg
     3241 accgaggacg cccacctgaa ggcgctgcgc cacctgcccg ccgcggtgca ggccgtcctc
     3301 gcgctcgagc cgcagatcat ggcctggagc gacgagttcg ccgccaagga gaacgcgctg
     3361 ttcctcggac gcggcctgca ctacccgatc gccctggaag gcgcgctcaa gctcaaggag
     3421 atcagctaca tccacgccga ggcctacccc gccggcgaac tcaagcacgg cccgctcgcc
     3481 ctcgtcaccg ccgcgatgcc ggtcgtcacc gtcgcgcccc atgacgcgct gctggagaag
     3541 ctcaagagca acatgcagga agtccgcgcc cgcggcggcg agctcttcgt cttcgccgat
     3601 accgataccc gcatcgactc cgacaccggc ctgcacgtca tccgcatgcc cgagcactac
     3661 ggcgtgctgt cgcccatcct gcacgtcgtg acgctgcaac tgctcgccta tcacacggcc
     3721 tgcgcgcgcg gcacggacgt ggacaagccg cggaacctgg ccaagagcgt gacggtggag
     3781 tgaggtcagc cgttctggaa cagctgatgt tctgaccaaa taaagtcgca aacacttcat
     3841 taaagtcgca aacaactcaa tagagtcgca aacactttca gcgtgcgaat ttgcgtctcc
     3901 agcacgatgc gcgtatgggt gagccgacgg cggctttcaa ccccgaagga gacaggctcg
     3961 acctgtacca acggtcagtt tccgctgcat tgcagtcgtc cgctatgcgc gtcgtcggga
     4021 tgacgccacg cgcggaatgg tcgatttctc gggctttagt gcacgacacc cgtaccagag
     4081 gcgcagaaca cgtgcgaccg gctctggttc atcacgttcc agttatacgt ccgcaggtga
     4141 tccatgtcgt cgaccgagta gtacacattg aatagcttcg gcatcgcgtc gatcacgtgc
     4201 gccggcacgc gctgcaagac cattcgacgc gggcgaaaca cgtagcgaat gaacgcgtcg
     4261 tgccgaaggt tgaagtcgat gctgtcgacg tacggcagat tgatcgagtt gctgaacccg
     4321 cggtcagaca gcagacactt ctcgctcgtg tatttgccga tccagaccag caccacgatc
     4381 tgcgggttag tgaacagcga ttccacgatc tgatcgaaga ggctgtcttg acccggcgcg
     4441 acatcgctaa gtagcatgaa ctgtgtgccg agccacacgc ggtactgctc gggcgtcaac
     4501 cccagcgttt tgcagatctg ctcttcatgc ggcttgttcc aggacgggtt cgagttcagc
     4561 gcgtcgctcc gcgtttgtcg gaacgagcgt cgacacgttc ctaaacgtgt cgagcatctt
     4621 ggccaccgca aacgggttcc gcatgaagtt catgagcttc gccttgaata cctcgaacac
     4681 ctcgaagtgc attccgtcgt tgtcattcgc agcggccttc cgcaacacag cctccgtgtg
     4741 ctcctgcacg ctgtcctcgt accgatgaaa aagagcctcg aagttctcac gcagcgtcga
     4801 gttctgcttc acgtcgaagc tgaacacgtc atcgagtgcc aagttgacgt ggatgcgcgc
     4861 gtgctgcggc tccctggcca caacagaagc gatcgtcgct gggaacaccc aacccccgct
     4921 gttcaccaac gtgatcattc gctgccaggc ggtcgatggc ggtcggaggc ctcacccggc
     4981 agcacgattc gcgcgagaag atcacgggcc tggactccaa gggcctcggc cagccgatcg
     5041 atgttgtcga tggagatgtt gcggcgcgcc tgctcgattg aacctatgta ggagcgatcc
     5101 agcccgcagc gcgatgcaag ttcggcctgg gtcaaattgc gcgcgtggcg caggttgcct
     5161 tctctgaagt ccaactcacg gcgcaagcag cgagggagac ggtaagcaaa accgtggacg
     5221 agatcgctga ttacagcgcc gccatcgcac gcgccaggac cctagactgg tcgcactacc
     5281 cctacacggc gatcgtcgtc cccggcatcg gcccggacga ccttgatgtg tcgctgagcg
     5341 cgcgcggcaa gctcaacgtc cggctggccg ccgcgcgctt cgcgcaggga ctcgcgccgt
     5401 tcaccctggt cagcggcgga cgggtgcatc cacgagggac gcactatgcg gaagcgctgg
     5461 agatgcgccg cgccctgatg gagcgcttcg gcatcccggg cgactgcatc gtcgtcgatc
     5521 cttacgcgag gcacaccacc acgaacctgt gcaacgcggt gcgtcggctg accgcgctgg
     5581 gggcacccct ggatcgcgac gcgctgatcg tgtcgaacgt cgaacagatc caatacatgg
     5641 tcagcgccga cttcgcatcg cgcaacgcgc gggagctcgg gtaccagccc ggactcgtca
     5701 agccacggac gtccgcgaac gagctcgagt tcatgccttc ggccatgtcg ctgcgcggcg
     5761 atcccgcaga tccgctcgac ccctgaaggc ttgcagctga acacgcctcg gtccgactct
     5821 gcggaggtgc gcctgcaggc cgaagacggt gcgccggcgc tcgtttgctg ctggcatgaa
     5881 cacctcttcg cgccgcgtgc gggtcacggt gcacagatcg attgcgcatc gaatgtcgta
     5941 ccgctcgctg atcgattggt gtcggtcttc gagccttttt cgctgatgtg ccgcgatgcg
     6001 tcgagacgcg tcgcgcacct gcggcatgac cacatgttgg cttgtccgca cggatcagta
     6061 gtagcaccga ttgagcatgc tgcccttgga ctttccaatt gcagacagtt gccttcatct
     6121 cattgaaggc acagcggcaa cagcgccgct gtacatggat ggctatgaag ccctcgtctt
     6181 gcgcactgcg cgggtcgagg gcttttttgt ttttggagtt cgaaacgtgt cgattccgtt
     6241 tgccgcgccg gcgatgaccg gccacgtcct cgcctcgctc tcgcgcgagg atgcgccgcc
     6301 ctgatcggcg tgcggccgtc gcgccgtcat cggcgcttac accaagaaat cgtgtcgtcg
     6361 tcgacgcgca tgcgcgacgg ctggcttcgc tcgccctgag atccgtgcgg ccctcgatgc
     6421 cgtgcgcaga cgctgcctag cggccgcgag gacctcaccg cgatgtgagc gacagaacaa
     6481 cttccggagg ggtcatgaat cgagattcgc ctgccaatcg cggaccgccg atgcgccgct
     6541 gcgcctgacc gttctccctg ctgcactccc tacgcgtccg ccttcgggat cggaccggac
     6601 ttgctcgcgc ctgattttcc gcgggccttc cctacaccca caccacgagg aatcctcatg
     6661 aacaagcaca cgctcatcca cgcctgcgcc ggcatcgctg ccgtcacggc cctgtcgacc
     6721 gcgagcgctg ccgacgcgcc cgccgtctcg ctgaccggcg acatcaaggc tgccgccacc
     6781 tacggaaacg gtggccaatc gccggtcgac ggcgcggctg cagaccacgt cgcgctcaac
     6841 gacaactcat cgtatttcag cctctccgca cgccaggacc tgccgggcgg gctctatgcc
     6901 ggcgtgcagc tggacagctt cttcggcatc gacactggca gcgatgcgac caacggcaac
     6961 ttcttcagcc gccgcgccgt cggcaagctg ggcggcagct tcggtgagat ctacttcggc
     7021 cgctcgttga caccggcgtc gcttatggtg ctgttcacgg atccctggta ctgggacggc
     7081 agcgtcgcgc aagcgggctg gatggtgcaa ctggccaact acaccagcac gcaatacctg
     7141 cgtacgaaca acacgatcgg ctgggtctcg ccgaagatgt cgggcttcac gctgagcctg
     7201 gccgcgtccg caggcgagaa ggtcaatagc aaggatctgg gcggcagcct cacctacgac
     7261 aacggcccgc tgtcggcggg catcgcgttt gaccagagcc acggcttctt caacgatccg
     7321 acgctgaacc gcacgatcgt cggtgtcgcc tcgtacgact tcggcgtagt caagccgatg
     7381 ctctcgttca cgtcgtcgaa ggtggccggc gtcaactact cgtccgtcgt tgtggctgcc
     7441 accgcgcctg ccggacagag cggtctgctg aaggcgcagt actcgcacat gaacgacgtc
     7501 gacacagcca ccgcgggtcg ccaggcctac gacaagttcg cgctgggcta ccagtacaac
     7561 ctgaccaaga gcgtgaactt cttcggacag gtctcggacg cgaaggagaa gtcgctgtcg
     7621 gcgaccaaca cgctcgagct cggcatggaa tacgggttct gatctcctgg gctcacggtt
     7681 cgcgacgggc gccgcgcgag ggcgccctgg cgaatgcgat gggtgttggc cgagctcggg
     7741 ggagctcggc ctattttttt ttgagcaatc gcgcgaacag cggcgtgaaa tgttcgctgt
     7801 tcgggcgctc gcaccgtccc cgcggcgttg acagcgcgcg ccacggtccc ttacgctgaa
     7861 tccacttcgt cgcagccttg cctggcgcgc gaacttcggc agcatcgccg aacacatcac
     7921 ggctatgaga gcccccgaat tgcgcatcgc gcagtcgggg gctttttcgt tttcgctttc
     7981 gacatgtgga gggtatgaac atgagcagag gaacgcgtgc ggcgtggata ggcgccgtga
     8041 tcgtgatcgc cgtggcggtg gctgttttcg tcttcttcga ctggacggca tggcgtggcg
     8101 ggccgtcacc gcaagggcca tcgagcgtcg cgccgccgcc cgtggtgatc ggcgtggcgg
     8161 tcggactgac cggcgcgaat ggtgtcgtcg cgccgaccgt ggtgcagtcg tcgcaactgg
     8221 cggtcgacga gatcaaccgc gcgggcggga tcctcggtcg acgcgtggac ctggtcgtcg
     8281 ccgacgacgc gtcggacccg gcaggcgtgc ccgcagccgc gcagcaactg ctgcagcagc
     8341 accatgcgga cgtcgtgatc gcgatggaga ccagcgcagc ccgcacggcg atgctcccgt
     8401 tcatcgagcg tgcgcgtgtg ccgtacgtct acacctcgtt ctatgaaggt cacgcgtgca
     8461 gtccctggat gtatgccaat ggctgggtgc ccgagcagca ggtggtgccg gtggtcgcgt
     8521 acctgatgca cgacaaggcg gcccgcagcg tctacctggt cggcagcgac tatgcgttcg
     8581 gccggggcat gctggagttc acgcgccgct acatcgagtc ccacggcggt cgggtggccg
     8641 gcgaggacta cctgccggtg gccgacaccg actggagcac gatcgtcgcg cgcattcgcg
     8701 cggcgaagcc ggatgcgctg atcagcgcga ccgcgggtgg cgcgcccaac gtggcactcg
     8761 ccaagcagct cgactcggca gggctgcacc tgccgtacgg caacctcgcg atcgacgaga
     8821 gcacagcgcg cacgatggga gatagggcga ccggcatgct gatgtcggct tcgtacctga
     8881 ccagcatcga ctcgaccgag aaccacgact tcctcgcaca catgctgatc cgcttcggcg
     8941 acaagctgct ggcgcccaac gagctctcgg tgccccagta cgaggcgatc cacctgtacc
     9001 gcttggcggc cgaacgcgca ggcaccaccg agtcccacgc cgtgctggct gcgctcggcg
     9061 cagtgacgtt cgatggtccg cgcggcctgg tgcagatgag cgccagccga cacgccgcgc
     9121 tggcgatgcg actggcgcgt gtgcaggcag acggctcggc tcgcatcctg cagaccttcg
     9181 gcaacacgga ccccggcgcg caatgccccg gcttctgacg atccgccttc acccacagga
     9241 gagattgaca tgacttggaa caaccggttc tcgcgcatcg gcgccaggct ggcgctcggg
     9301 ttcggcggcc tgctcgcgtt gatgtgcgtg atcgccgcga ccagcggctg gtcgctgctg
     9361 gccaccggcg cgcaggtgcg ccagatcgtg gaggtcaacg accgccgcgt gtcgcaggcc
     9421 cgcgaccagc tcgacgccat cgacgccatg acggccgcgg tgcgttcgat cgccctgttc
     9481 accgacatgg acgcattgaa gtccgagaac gccaagctcg aggcggcgca gcgccgctac
     9541 gacgaggccg cgcatgcgct cgaggcgtcg ctggccgacg cccccgacga cgaacgtgcg
     9601 ctcgccgccg agatcgcccg cctcggtcgt gacggcgagt cgatggttcg cagcatcgcc
     9661 aagcagggct tggcgggcgc caacatcgac gtgacgaccg cgctgacgca gacgctgccg
     9721 ccggtcgaag ccacgtggcg tgcgaaggtt ggcgccctgg tggaactgct gtcgcggcgc
     9781 aacgctgaga tgtcgacggc ggcgacgcag cgcacccacg atgccacgct ggcctgcggc
     9841 gcgctggcag tgatcgcgac cgtggtgggg ggctttttcg cctgggtgat cgcgcgcggc
     9901 atccagcggc cgctggccga tgccgtgcgc gtggccgagc gcatcgcagc cggggtgctc
     9961 gacagcccga tcgagtccgg cggcgccggt ggagcgagcg agatccgccg gctgttgggc
    10021 gcggtcggcg cgatgcaggc gcggctgcgc gacctggtgt cggcgatcct ggaagccgcc
    10081 ggcagcttgc aggtcgccag cgccgaggtc gcggcgggca atgcggactt gagtcgccgc
    10141 accgaacacg tggccgctcg tctgcaggag gcggcaagcg cagtcgaacg gctgtcgacc
    10201 gacgtgcgcc atggcgtcga tgcctcggcc aaggcgagcg agctcgcgcg cgaggcggcg
    10261 cagatcgcgg ccaccggcgg cgaggtagtc gacaaggtgg tgtcgacgat gacgcggatc
    10321 agtacatcgt cgcagcgcat ggccgacatc atcggtgtga tcgacgggat cgccttccag
    10381 accaacgtct tggccctgaa cgcggcggtc gaggcggcgc gcgcgggcga cctggggcgc
    10441 ggcttcgcgg tggtcgcggg cgaggtgcgg ctgctggcag gacgcagcgc ctccgccgcc
    10501 aaggagatcg gcgagctcat cgaggcgagc atgcgccaca tcgacgaggg ggcgacgctg
    10561 gtgcgcgatg cgggcggtgc gatggggcgc atcgtcgtcg gtgcgcgcca tgtgtcggac
    10621 atcatcggcg aagtgagcac cgcggcgcgc caccagagcg cgcgcgtcga cgagatcagc
    10681 gtggccgcct ccgaggtcga ccaggcgacg cagcagaacg cggccctggt cgaacagagc
    10741 gccgccgcgg cggacagtct cagagaccag gccgatcggc tcgcgcagct cgtgagcgca
    10801 ttcgagctgg gcgatcgcgt tgcgcccgcg cgcgacggcg tcgatgccgt cgtcgcgaat
    10861 tgatcgcaca tcagtgcaat ctcggcgttg acacttccgc tcgccatgcg taatctagcg
    10921 tcatcgtctt tcgacctgca tggtgaaagg cgcgtcggca agactgccgg cctcaacaac
    10981 ggctacgaag cccctgattc gcgcaccagc gcagtcaggg gctttttctt tttgcattcc
    11041 gatttcgaca tgttcccttc cggtactcct ggcgaacgca gcgccttccc cgatatcgtc
    11101 gtcgcgtgcg tgcagatggc gccgcaggtc ggccgcaagc acgacaatgt ggggcgcggg
    11161 ctggcggcga tcgaacgcgc agccgaccag ggcgcgcaac tcgtcgtcct tcccgagctg
    11221 tgcaacaccg gctacgtgtt cgacagccct gaagaggcgt tctcgctcgc agaggacgtc
    11281 cgcgacgggc agaccgtgca ggcgtggatg gacgccgccc ggcgacgcga cctggtcttg
    11341 gtcgcgggcc tggccgagcg cgacggcgat cgcctctaca actcggcggc agtgatcggg
    11401 cccggtggcc tcatcggcca ctatcgcaag ctgcacctgt ggaacgagga gcacctgttc
    11461 ttcgagccgg gcgatctggg cctgcccgtc ttcgatacgc cgctcggccg catcgcgccg
    11521 ctgatctgct acgacggctg gtttcccgag acctatcgct atctcgccca acgcggcgtc
    11581 gacatcgtgt gcatgcccac gaactgggtg ccgatgccgg ggcagtcgga tgacgaacgc
    11641 gcgatggcca acacgctggc catcgcaaac gcccacgtga gcgcggtcaa catcgcctgc
    11701 gccgaccgcg tgggcaccga gcgcgggcag cgcttcatcg ggcgcagcct gatcgtggcc
    11761 gccggcggcc aggtgctcgc cgggccagcg agcgccgacg acgaggagct gctgctcgcg
    11821 cgcctgaatc tcgcgagctc gcggcaggcg agccgcttta acgctttcaa cgatgcgctg
    11881 cacgatcgtc gcggcgatgt ctatggcacg gtgcacggcg acgactggcc gccgcaatgc
    11941 tcctgaacga tcacgccagt ccacaaagag gaacgaccat gaacaagagc tttcgtcctg
    12001 tcctgaactt cggcgccacc gcggcgctgg ccgtgctcgc gctgggggca cgcgccgaag
    12061 agccgatcaa gatcggcgta gccgtcggcc tgtccggcgc caacagcgtc gtcgcaccgg
    12121 ccgtggtgca gtcgtcgcag ctggccgtcg acgagatcaa tgccgccggc ggcatcctcg
    12181 gccgcaaggt gcagttggag atcgtcgacg atgcctcggg cgcggtcggc gcgcagaagg
    12241 cttacgatac gctggtcttc cagaagaagg tcgatgcggt catctcgatg gagacgagcg
    12301 cggcgcgcaa cgcggcgctg ccggccatca cgcgcggcaa agtgcccttc atctacacgt
    12361 cgttctacga gggacactcg tgcaatccgt ggatgtacgt caacgcgtgg gttccggagc
    12421 agcaggtggc gccgatcgtc aactacttct ggtccaccct gaaggcgcgc aactacttcc
    12481 tggtcggcaa cgactacgcg ttcggccgcg gcatgctgga gttcacccgc aagtacatcg
    12541 agaagaacgg cggcaaggtc gtcggcgaag agtacctgcc gatcgacggt tccgactgga
    12601 cctcggtgat cagcaagatt cgcgaagtgc atcccgatgc gctggtcagc gccaccgccg
    12661 gcggtgcgcc gaacgtggcg ctggccaagc aactgcaggg ctcgggccta gcgattccct
    12721 acggcaacct cgccatcgac gaaggcaccg cgcacaacat gggcgacgcg gccaaggaca
    12781 tgtacatgtc ggcgtcctac ctcaccaaca tcgacacggc gggcaaccat cacttcgtcg
    12841 atgccttgaa caagaagttc ggcggggaac tgaagacgcc caacgagctg tccgagccgc
    12901 agtacgaggc gttcttcctc tacaaggcgg cggtggagaa ggccaagtcg accgaccatg
    12961 ccgcggtcgt caaggcgctc ggcgaggtgt cgttcgacgg cccgcgcggc gacgtgcgca
    13021 tgaaccgcag ccgccatgcg ccgctggcga tgcgcctggg ccaggtgcag gccgatggct
    13081 cgatccacat cctgcagacc ttcaagagtg tcgacccggg cgagcagtgc ccgctcctga
    13141 agtaagcgcg atccggggcg acgtccgcgc caggctggcg ggcgtcgccg ccatctagga
    13201 aaaagatatg gctctcctac tcgacatcat cacgaccgcc gcggtgctgt tcatcgtcac
    13261 ctcgggtttg atggtgatct tcggcgtgat gaagatcgtc aatttcgcgc acggcgcgct
    13321 gctcacgctg ggcgcctacg cgagcctcgt cgtcacgcgc ctggcgctgc cgccctggct
    13381 cggcatgccg ctggcactcg tggtgggcac ggcggccggc gcgctcgtgg agcgcttcgt
    13441 gatccgcccg ctctacaagc gcccgctcga cgccatcctc gccacctggg ggctgggcat
    13501 cgtgatcggc caggtgatcg ttgcggtgtt cggccgcgag gtgcagttcg tccaggcacc
    13561 gctgtcgggc gctgtcgcca tcgccggcac gacctattcg gcctatcgcc tcgtgctggt
    13621 gccgatcgcg ctagtgctgt gcgggctgct gctggcgctg ctggccggca cccgcttcgg
    13681 cctgaagaca cgcgccgtca tcatgaacga ggatctcgcc gagagcctgg gcatccacgc
    13741 cggccgcgtg cgctttgcca ccttcgcgct cggggccgcg ctgggcacgc tcgcgggctg
    13801 cctcatcacg cccctgtcca gcgtcgatcc tggcatgggc ctgacctggc tcgtgagcgc
    13861 gttcatgctc gtgatggtgt cgggccactc gatggccagc ctgctgctca cctgcctcgt
    13921 gttcggtgcc tgccaggtcc ttgtcagtgc ctacgcgaac ccggtgctcg ggggcatgac
    13981 gatcgcgctg ctcgcggcgc tcacgctgcg catccgtccg gcggggtttg cccgtggatg
    14041 agactctcat gatcattccg aaaatccagt cgcccgcgac gcgttctcgc gccgcccgcc
    14101 cactcacctt tgtccttgcc gtgggcgcga tcgtgctcgc agccggcccc tggctgttcg
    14161 acgactacct gctcaacgtc ctggtcaagg ccttcttctt cgcgatcgcg gcggtgaccg
    14221 tcgacctgct gtggggctac atgggccacc tcacgttcgg gcagtcggcc ttcttcggcc
    14281 tcggtgcgta tgcctcggcg ctcgtcttca cgcaccacgg cctgtcgtgg ggcacggcgg
    14341 tcatcgcgct cgggctcgcg gtcgtcgtgc cggcaggggt tgcggcgttg ctgggatggc
    14401 tgtcgttcta ccgcggggcg tcgccgttct tcgccaccgt gatgtcgttg gtgctgccga
    14461 tcgtcgccag ccagctgttg ctgtccggcg gagaatggac cggctcgagt tccggcctga
    14521 ccggctacga gacgcctgac ttgccgctgg gggcctggta cgccatcgcg ggcctgttgc
    14581 tggccggcgc gggcgccctg ggctggatgg ttgtgcgagg cgacgccggc cgcgtgctgg
    14641 tggccatccg cgacaacgag ctgcgctgtg cctatctcgg cttgcgcgtg catgccatca
    14701 agatcgtcct gctcgtggcc accgccgtcg tcgcgggcgt ggccggattc ggctacggcg
    14761 cgttcagcgg tgtcgtcgcg cccgagctca ccggcttcgt gttcggcacc gagctcatca
    14821 tctgggtcgt gctcggcggt cgcgccacgt tgtgggggcc gcttgccggc gcggtgctga
    14881 tcaatgtcgc gacctcctac ctgagcagca gtctgccgtt cgcgtggccg ctggtcgtgg
    14941 gcgtggtctt cgtcgtcgtg atcgttctgc tgccgcaagg cgtcgtgccg ctcgcggtgc
    15001 ggctggtcga ccgcgggcgc tcgccggtat cgccggcgcc cgtactgcgc gaggccgcgc
    15061 cagcggccaa gccagcgagc gatgggccgg ccttcgagat gcgcgacgtc gccaagcgct
    15121 tcggttcgct tgcggtgctg cagggcatcg acttgcgcgc ggacgccggc gacctggtcg
    15181 gcctcatcgg acccaacggc gccggcaaga cgacgctgat gcgctgcatg agcgacggca
    15241 gcgagcgcag cgccggcgac gtcaggctgt cgggtcgcga catccgcaat gccgcgccgg
    15301 acgactgcgt gcgcctgggc ctgggccgca agttccagaa cgccaacatc ttcgagtcgc
    15361 tgaccgtcgc cgaatgcctg cgcatggcac gcacgatcgt ccaggcaccg cgacttctcg
    15421 gccgggcacc gacgctggag ttgccgagct atgcgctgga ggtgatgcgc gtcaccggac
    15481 tggaggccaa gctcgcggtc gtcgcgcgcg acctgtcgca tggcgagcag caggcgctgg
    15541 agctagctat ggtgctggcg ctggagccgc gcatcgtgct gctggacgag cccacggccg
    15601 gcctgaccaa gaccgagcgc aggcagatcg gcgaagtgct gtccgcgctc gcgctgcgcc
    15661 acggtcgctg ctgcctgctg gtggagcacg acctcgattt cgtcgaggag atcgccaccc
    15721 gcattgtcgt gatgcaccag gggcgcatcg tgatggaggg cccgttccgc gaggtcgtgg
    15781 agtcggagct cgtcaagacg atctacgcag ggtcggcagc gccggcccgg gaggtggcgt
    15841 gatggcgcgc gcattggaca tcgaaggtgt cagcagcggc tacaaggcct cgctcgtcct
    15901 gcgcaacgcc tccttgacgg tggccaaggg cgaagccgtc gcgctgctgg gcaagaacgg
    15961 catgggcaag agcacgctga tgaagacggt gatgggctac ctgcccaagc agtcgggccg
    16021 cgtgcacgtg ggcagcgacg acgtcacgcg cgcctcgccg caccaggtcg cggcatgcgg
    16081 cgtcgcctac gcggcgcagg agcggccgct gttcaatgac ctgagcgtgc gcgacaacct
    16141 gcgcctgggc ctcgcccagg agcgcttgtt cgacgagcgc ttcgccgaga ttgagccgct
    16201 gttcccggtc ttcaagagca ggctgcgcca gcccgcgggc acgctttcgg gcggcgagca
    16261 gaagatgctg ctggtggcgc gctcgctgat gatgcgccct gcgctgatgc tgctggacga
    16321 gatcacggaa ggcttgcagc cgtcggtcat cgaccgcatc gcgcatgcgt tgcgctggga
    16381 gcgcgagcgt cacggcacgt cgatgctgct ggtcgagcag cacgtcgcct tcgcgctcgc
    16441 ggtggtcgat cgctacgtcg tcctcaagcg gggcgaattc atcgattcgg gcgatgcccg
    16501 tgcgcaagga gcacgcgatg ctgtcttcga tcatctccag gtctaggcca atgacgctat
    16561 ttgcagctgc cacagagggg ctgccgcgtt gcgtcgtgat cgcgacgggt ggcaccgtgg
    16621 ccatgcggcc cgacccgcgc agcggggcgc cagtgccggc gctgtccggc gaggaactcg
    16681 tggccagcgt gccgcagctg ctcgggctcg ctcgcctcga tgtgcgcagc gtcttcaacg
    16741 tgccctcggt ggagatgggc cccgcgcgct ggctggcgtt gcgccaggcg ctgctcgcgg
    16801 cgctggacga cgacgaggtg tgcggcatcg tcgttacgca cggcaccgac ttgctggagg
    16861 agacggcctg gtttctcgcg ctgacgctgc ccgccgaatg ccccgtcgtg ctgacaggcg
    16921 ccatgcgcca cgcggcggag agccagttcg acggcccacg caacctgctc gacgccgtgc
    16981 gtgtcgcgat atcgcccagc gctcgtggca tgggcgtgct cgtggtgatg gatggcgagg
    17041 tctattgcgc ccgcgaggcc gagaagtcgc ataccaccga gacgagtgct ttccggcgcg
    17101 ggcccggcat cgaactgggc gcgatcggcg ccgaagggcc ggccttctgg cgcaagcctc
    17161 ggccgcatcc ccgcatcgag cccgccggcg gcgacatgcc gcgcgtagac atcgtcccga
    17221 tgttcggcgg agcggacggc ctgccgatcc gctgggccgt gcagtccggt gcgcgcggcg
    17281 tcgtcgtgca ggccgtcggc gccggcaacg tcagcgccgc gatgtacacc gagctgctga
    17341 acgcgctgga ggccggggtc atggtcgtgg tggccacgcg cgtgcgcggc ggccgcagcc
    17401 agccggtcta cggtttcctc ggcggcggca cgctgctggc tcaggccgga gcgatctttt
    17461 ccggagggct gtcgccgcag aaggcgcgta tcctgttgat gctggcgctg caaccgcgct
    17521 ggtcgacggc gcaggtgcgc gaactgttcg atttctgaga cctggttcct gtcgcgggag
    17581 atgtccttgt cacgagaaga tgcgtcgacg tcgaacgccg gctggcgggt cgggatcctt
    17641 tactcgcgct cgggggccac gagcgtcacc gagtcggagc atttcttcgg cacgacgctg
    17701 gccgtcgagg agatcaacgc tgcgggcggc gtcctcggac gacccctgct gccggtggcc
    17761 tacgatccga agggcgacag cggcgagtac cggcgcttgg ccaaccggct cgtgatcgac
    17821 gacgacgtga acgtcatctt cggctgttcg cgctcgtcca gccgcaaggc cgtgctgccg
    17881 gtggtcgagc gcaacaacgc gcttctctgg tactgctcgt tctacgaggg cttcgaatac
    17941 tcgcccaaca tcgtctacac cggcgccgtg ccgaaccaga acagcgtgca gctggcggct
    18001 tacctgctgc agcattgcgg cacgcgcctg ttcctggtcg gcgcggacta catctatccg
    18061 cgcgagtcca accgcgttat gcgcgatgtc gttgagcggc acggggggga aatcctcgac
    18121 gaggtctacc tgcccatcga ggccgaacgt gccgagctcg aggaggtgct ggacgacatc
    18181 gagaaggcgc agccgagcgt ggtcttctcg acgctggtcg ggcaatccgg gcggctcttc
    18241 tacacgatgt tccatgaacg cggcctcgat gcgaatcgca tgccgatcgc cagcctgggc
    18301 atggcggaag aggagattcg tctcgtcggt ccggagcgct gcgtcggaca cattaccgcg
    18361 gcgacctatt tcagttcgct gcagagctcg gccaacgatc acttcaagaa gctgtggcac
    18421 gaccggttcg gcgagcgtcc gacgagcacg tggtccgaga tggcgtactc gcaggtacac
    18481 ctgttcgcgc gggcgctgga gcgcagcggg agcctggaca cggtcaagct ggtcaacgcg
    18541 ctgcgcaagg tgcgcttcga ctcgccggaa gggccgatcg cgatcgacgt cgacaacaac
    18601 catctcgcgc ttacgccacg catcggcagg tgccgcgcgg atggccaatt cgacgtcgtc
    18661 tggcagagcg cggagccgat ccgtcccgac ccgtacctgt cgacgttcgg gttcgccaac
    18721 ttctggctga agtagcacca tgtcgagtgt ccgtcgcctc tacgaggacc tgcgcagcat
    18781 cagcgtggtc gtgatccatc cgccgggcga ggatcgcgac gtgatcgtgc agcagttgca
    18841 gcgcatcggc tgccgcttgc ggaccgtgtg gccgttccct gcggagccgc cgtcccaggt
    18901 ggacgtggtc ttcttccagg tctcgcagga tctggccgac cacggcggct ggtcggcgag
    18961 cgcgctcgat tctgccgagg tcacgctggt ggcactgtcg gactacgaga acccgacgac
    19021 gctcaagctg ctgctcgatt cccatgcgca cggcgtgatc accaagccgt tccgctcgag
    19081 cgggattctc agcacgctcg tgctcgcgag gtcggcgcac agcttccagc aaagaatgca
    19141 gggcaagatc gacaagctgg agacgacgat gaaatcgcgt cgtgtgatgg agaaggcgat
    19201 ccgcctgctt gtcgaccacc agaagatgtc cgaggtgcaa gcctacgagc acatgcgatc
    19261 gcgtgcgacc agcctgcgcg tgaccgtggc ggaagtcgct gggatgatga tcgatgcgca
    19321 ggaggccatg gagaagctcg ggcttcgagg ttgatggcgt gccgtggctc cgacggtcct
    19381 tctcttgccg tgtcggggaa gcgacatacg agcgaagatt gggcttcccc cggttctccg
    19441 gacacatcga atgacatgat gtgtactgga gagaaccatg agcaagccaa gaaggcattt
    19501 cagcgacgag ttcaagcgtg aagccgtgag gatgtgcctc gaaccggggg cggtcattct
    19561 gcaggtagcg cgtaaccttg ggatcgatca gagcgtgctg cgccgatggg tgcaggccga
    19621 gcgcggcggc aggctggaga tgaattcggc gaagcccctg cgcagcgagg cagccacgga
    19681 gctggagcgg gtgcagcggg aactgcgccg agtgacgatg gagcgcgaca tcctaaaaaa
    19741 agcgctcggc tacttcgcga aggacccgca gtgaagtacg cctggattgc caagcagcgc
    19801 gggacgtggt ctacgcgggc gatgtgtcga ctgctggagg tctcgcacag cgggttctac
    19861 gagtggctcg gacgaggccc gagtgccagg tcgcttgagg atgccaggct cacgcgcggc
    19921 atccgccaga gtttcgaact cagcgaccgg acctacggta gtccgcgggt ctggcacgac
    19981 ctacgtgctg caggagagcg gtgcggtcgc caccgggtgg cccggctcat gaacgcagcg
    20041 aacctcaagg ctcgaaccag gcgtcggcgt atgcctggcg atggcgcgca gcgcctcgag
    20101 cacaacatcg ccgagaaccg cctgcagcgc gaattcgaag ccgacggccc gaacaagaag
    20161 tgggtcgcgg acttcaccta cgtctggacg gccgagggct ggctgtatgt cgcagcggtc
    20221 atggacttgt actcgaggcg catagtgggc tggtcgatga gcgagtcgat gcacgcgaag
    20281 atggtgaccg acgccctgct gatggcattg tggcggagag gacgtccctc gcagctactt
    20341 ctgcactcag atcagggcag tcaatacacg agcgaagagt tccagtcgat gctgcgggcc
    20401 gaaggcatca cgtgcagcat gagccggcgc ggagactgct gggacaacgc ggcgatggag
    20461 agcttcttca gcagcctcaa gaccgaacgc ctgagccgca aggtctaccg cactcgcgac
    20521 gagctgcgag ccgacgtctt cgactatgtc gagcgcttct acaaccagca gcgccggcac
    20581 tcgaccctgg acttcgttag cccagtccag ttcgaacaag gtctaatggg ctaaccaagg
    20641 cgtccggaga accgggggaa gcccaatcgt cccgagttca ctgacttggg cgagtccgcc
    20701 agcgcaagcc cgaagacctc gcgcgcggtg ccgggtggac gttcagccgt tctggaacag
    20761 ccctgactcc ttcatccgcc ggatccgcgc gcttcgcgtg cgtccgtgcg gagcctccac
    20821 cggcgaggga gaagcgaagg gcgcggatcc gcgggggtcg gggccctcgc gcgcaggcgc
    20881 cgcgagcgcg cgcgcaggcg gatccacgat cgcggctccg ttcatctcgg cttcgtcgac
    20941 gacgtccacc agcaccggca cgtcctgcgg caccaccggc gcgtggtcga tcaccgatcc
    21001 cgcatccacg gccgacgccg ggtcgccgcc gaggtcgacc ggcggcgggg gcggatccgc
    21061 aggatcgacg gcctcgggca cgctgaaggc cggcccgtgc aggcgcgcga tctcccagat
    21121 gagcccggtg gccgcgagct ggcgcagcct ctcctggcgg gcggcgctgc gctcgatcga
    21181 cgccagccgc gcgtagagct cgggatggac gtcgctgtcg atctccagct cgagatgcag
    21241 cgtggccatg tcgcgcgctt caggactcgg cgaccgcctc gccgatcatc tggaagcctc
    21301 gcacgttggc agtgaccgac tccggcatca cgaagacggg aatcgccggg aagcgctgct
    21361 tgagcacgtc gcggtagcga tccggatgtc cgcccacgag cacgatcagg tcgacgatct
    21421 ccgtcgcatc gcgcaggccg tcgatcatgc ggttgacggc gtcctcgatc acgctcatca
    21481 cccgcggttc gtacctggac aagtcatatg cgacgccgga cagcttgacc ggcgtcttct
    21541 tgcgcagcgc ctcgttgatg tgcggctgca cggacacccc cagcggccgg ccgacgtcct
    21601 ccgaaagcgc cgcctggacg gccttcagca cccggtggcg gccggcgtcc gaggcggcgt
    21661 tgctggccga cggattgatc gtgccctgct ggatgaggag ccagtccagc gtgtgctcgc
    21721 cggggtccac catcagcacc gcaagctggt cgagatcgtc gacggatgcc agcggctgca
    21781 gcgcgccgcc ggtcgccgcg atctcccggt tgagctcgtc caggtgctcc tccagcgccg
    21841 cgtagccgcc catcggctgc gcctgcacct ccacccgggc gaccgtcacg gtcttgcctc
    21901 cgccgacatc gagcgggccc tcgtaggtgc ggcggaggta gctgcgtctc gcggtgtcgt
    21961 tgaactggtg gacgggcaat cccagcacga ggacgtctat cgtcgagtcg cccgcctcgg
    22021 ccatgtaccg cagggcgccc ttcgtcaccg cttcgtacac ctttcccttg tagaactcgt
    22081 cggtgacgtc gcggccgaag ctgcccccgg ccaaggccag gccgacatcc tgcccgacct
    22141 cgtagagcgc gccctggacg gggacgtcga acgtgtcgcg gcgccgcgtg ctcagcgagc
    22201 gcaccgcgct ggtgtcggcc gggatcgcga gcgagggaaa gctgcggaag ccgacatcgc
    22261 cgccggcggc gggcagcgag aacttcacca gcccgaagcc gacgtcgatg gcgcgtacca
    22321 ccgggccggt gctcgactgc gacgctgcgg ccggggcggg ctgctgcgca ggagcggcgt
    22381 gcgggccgcg ggacggcgac gtgtcgcgga ttttcgaaac gttcggttgt gccatggatt
    22441 ctcctgttga ccatgccggc ctgctggcct ggctcgccgc cgcgaggctg ctggcttctc
    22501 gacagtcgca cgacgtgcgc acgcgcggca attcctcctc ccggtgcggg ccagcgccac
    22561 cctggcgtcg atgccgcgtg cagagaggct ctcggcgctg cccgcatcgc cacgagggga
    22621 cgccgcgagg cgcatgcaat gcgtacggtc gagtaaggcc gacggcaagg cgagctgtcc
    22681 gtgctgcgca gcaggggtcg ccggcgtgga tcgaccggag cggacgccct gcgcgtcctg
    22741 cagcgccccg gctgcgccgt ccatgacact ccctgccata ccccagcgca gtaattcacc
    22801 aaggggatcg cacggcacaa gcgacagcaa gaatgccgca cgctgggata tcgcatgggg
    22861 ctgcggcgcc agcggaggcg tccgcggggc gccaacggcg cagcctcagc tcagtcttcg
    22921 ggcggcggcg gcattcgctg ccggcccgag atcggatcga tgttcgacct cggtgccgcc
    22981 ggcaggtggg cgacgacgcg gcgcgagacg gtgtcgacct cgaagcgttc cccgacctcg
    23041 ttctcgatca gcagcttgcg accgtcggct cgcatcgacc ggaaatagac atcctgcaca
    23101 tcgccttcca cgcccggcat tcgaacgttc tcgtagacgc gcaagcgcca cagctcccgg
    23161 ttcgaggcgt cgaaggcgcc caggataccg ccaatctggc cgtcccgatc gatgtcgccg
    23221 atgacaggtg cataacgcac accgtcgatg acgacaggag gcactcgctg gggcggcgca
    23281 cggctggcgg tgggctccgg cttgctcgac atggccttct ccttggtgcc cgcgtcgcaa
    23341 catggggcgg cgcaagcgat caacgccgcc gcaattgcga cagcatggtt gttcatcgtg
    23401 cttgcgcaac gaccgtgcgc acgaccttct gggtaggcct ccagccggtg tccgcgtaga
    23461 gcttgctgcc cagtgcggcg atgcggtcgc ggtccgtcaa tgctccggcg gcgtccgcgc
    23521 agaaaaacgc ccagctgtcc gcgttcgtca ggcactgctc catcgtgagc gtgcgcactg
    23581 cggaaaaccc agcgtcctgc gctacacgcg cgtcgttgac acggcgaaag acatccgact
    23641 cccgtggcag caagccttgc caactgtagc tatggtcctc ggtctggcag tagttgtgcg
    23701 tgagctcatg cacgacgatg cgcgcccagt tcgtggcgcc cgtcagcgtg ttgcctccgc
    23761 cccagaaacc tggctcgacc catacggcgt gaatgtcatc gcgcgggaag gtgtaggctt
    23821 cgctctgttc ccatttgctg ttggtgccgc gctggccggg gtcatcgaga atgatcaagt
    23881 cgccgacctt caagccgatg gcgatcttga gcaggccacg acgcagcgtg ggcgtgaatg
    23941 ccgcaatggc gccgtcggca tccggatggg tcgcaggcac gaaccagcgc ttcaacaggg
    24001 tcatatgctc gggtcgcgtc ggatcgccag ccacgatcat tgctcgctcg acccagcgaa
    24061 gggacattga cacagcgctc tcgatgcggc gcttctgctc atcggtaaat atctcgacgt
    24121 tttgttccaa caactggtcg accacggcgt cgggcgactt cgcatattga atcggatggg
    24181 cgacatatgt ggccggaagg gaaagtatcc acaacttccg ctcgccgcca ctttcgtaaa
    24241 aatagaggtg cgacagcgtc ttcagcagaa gcgcccgcgc gcaagcattg tcggaagggt
    24301 tgctcaccaa gctgccttcg gccttcgccg ccgtccggat ggccttggac tgcgacttgg
    24361 cgcccgccag cttctcgcgg atccgctcgg gaatcccgcc ctggagatag ttgaagccgt
    24421 catcgcgcac gaggttcggc aacgcctcgg caatcacttt ccagtcgttg gcgtatttgt
    24481 cgggcgtctg cgtcgccgcc acgagcttcc tgtgaatcaa tagatacgga acggggttcg
    24541 ccaccaagcc ctccttgtgg aatcatgcgc cggcccgagc aggcacggct ggagccgtgg
    24601 gagcttatgc agcgttgccc tcagcgacaa ccgccgccac aagcctcccc ctatccgaag
    24661 tcgtcggtcc agggcagcgc cttcgctcag cccgcgctgc ccagccccgc gaagacgttc
    24721 tgcacgtcgt cgtgctcgtc gagcgccgcc aggaaggtct cgacctcctc gagcgcctcg
    24781 ggcgacagcg acgccggatc gaccggattc ttcgccttgt agccgagctt ggcggacagc
    24841 accttgaagc cgtgctcggg gagcgcgcgg ctgacgaggt cgaggtcggt ggggtcggtg
    24901 aggaacagcg tcgtgccgtc ctcgtcaccc ggctcgacct cctgcgcgcc ggcttcgatg
    24961 gcggcttcct cgggatcgac gccgggcgtc gccggctcgg cctcgatcat gccgacgtgg
    25021 tcgaagtccc aggtgaccga gcccggcgca ccgagctggc ccttgcggaa gatcacgcgg
    25081 atctcgggcg cggtgcggtt gacgttgtcg gtcaggcact cgacgacgac cggcacgcgg
    25141 tgcggcgcga agccctcgta gaacacgtgc tcgaagttca ccgtctcgcc ggtcaggccg
    25201 gcgcccttct tgatcgcgcg ctcgagcgtg tccttgggca tcgagacctt gcgcgcctgc
    25261 tcgatgacga ggcgcagctt cgcgttgccc gccgggtcgg cgccggcctt cgcggccagc
    25321 atcaggtcct tggccagctt gccgaacagc ttgcccttgg cgttcgccgc cagttccttg
    25381 cccttggcct tccactgcgc gcccatgtct cgatcctcgt gttgggtcgc cgcaaggcgg
    25441 gcctgcggcc gcgcgcctgc cggcgacagg agaaagccgc attctgccgc agcgctcgtc
    25501 ggtgccgacg agcgaccgcg ccgcggtacc ggggcggcgg agcaacagga tcgtggccgg
    25561 tccctggcag gatgggcgat ccctcctgct cgtcgtccgc atgttcctcg tcctcaagtg
    25621 cctgctcggc gccgccgccg tgctgctgat cgccctgctc tcgcgcagcc gcaacttcca
    25681 tctcgccggc ctggtgccgc tgttccccac attcgcgttg atcgcccatg ctgctggccg
    25741 catggccgaa atgaagccgt cctagtcctc gaagcggctc gacgcgtcgc ggtcgcgctc
    25801 gtagcgatgc gcgagcggga acggcggcag ccaggactcg cggcggatcg tccacagctc
    25861 gtaggtcggc gcgaagaggt cgggcgcgtc gagcgcgccc aggctcacct cgacctcgtc
    25921 gccgctgcgc gagaacaccg acgagccgca gcgcgggcag aacgagcggc cggcgaactc
    25981 gcgcgtctcg ccttcgatcg ccaccgcctc gcgcgggaag atcgcggacg cgtggaacag
    26041 cgcgccatgg tgcctgcggc aatcgaggca gtggcacagg ccgacgcggt acggccgacc
    26101 ggaggccacc acccgcacgt cgccacacag gcagccgccg gcgacctgcc cgccgcccgc
    26161 gccggcacgc ggctgcgtca ccgcgacgcc tcgccttcca ccaggcgcct gccgaccagc
    26221 cgttccagca ccttgagcgg cgacttcgcc gggtcggcca tcgccgccag cggcaggtgg
    26281 aaggtctgct ccagcatgaa ctggccgctc atcgcggcgt gcgaggtctg gtcggagaaa
    26341 cagatccagg cgctgcccgg cgggaaggcg acctcctcct gcggcgagtt cgcctggtag
    26401 cccatgtccg ccttcatcgc gtcgtgcagg cccagcatca ggtggtcgta gcggctgcgc
    26461 gggctcttgg tgacgtgcag cgcgttcagc agccaggccg agccgggcag cggcgggcgc
    26521 agctgcggca ggaagcggcg tgcgatgtcc tcgaagggct cgcccacgcg ccacacgcgc
    26581 gccacgccgt gcggattgat gttgaggaag acgcgcagga tgcgctcgcc ccggttggga
    26641 cgcgacggaa aggcgtcgac gtgcaggcgg ctgtcgtcct tgcgccagga cagcgccttg
    26701 ccctcgatcg gatgcagccg caggctcgcc ggcgcagcgc gcatgcggcc gcggtagtgc
    26761 gggaacaggc ggtcgatcag ggcctgagcc gtctcgtggt agcggcgcac caggccttcg
    26821 gccgtgcggc gcgcgtcctg cgggccgacg aatcccgcaa ggccgccggt cttggggtcg
    26881 aagctgatgt tcttgcggtc cgggtcgacg ttggattcgt cgagcacgcc cagctcgtcg
    26941 gccgacagcg ggaacgggaa ggccggcatg tgcagcacga ggccgcgctc gagcgcgtcc
    27001 gcgagccgcg gcgagggcgc ggcggcgtcg cgctcctcgt ccgggaccgc gatgacctgg
    27061 gttcgttgat ccatgggcgc gatggtagcc gcaacgcgcc ggcacgcagg tcagccgccc
    27121 agcagccgag cgagcagcca ggccggcagc ccgaggtaca gcccggccag gcccagccgc
    27181 tcgagctgcc gccgcgcccg ccccgcgcgg gccggggaca gcagcacccc ggcgacgg
//
LOCUS       DASVLN010000144         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_437620, whole genome shotgun sequence.
ACCESSION   DASVLN010000144 DASVLN010000000
VERSION     DASVLN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437620"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VF453_03290"
     CDS             complement(<1..362)
                     /locus_tag="VF453_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686701.1"
                     /translation="MPAPTLRPAADPLDAMDRSTDRGVDAAMARQERADLPGERSDQP
                     RPDKAGDVYLDAARDLVEGERDPEQARRDDDREAERVADGDPDIAGIGADDDIEAGAP
                     ARGADDDDPIDETERAGD"
     gene            complement(422..712)
                     /locus_tag="VF453_03295"
     CDS             complement(422..712)
                     /locus_tag="VF453_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7686702.1"
                     /translation="MLNLLNIAVWLVVGMVVGAVAGLLMRHEEDAAVFPNVAVGIVGA
                     LAAGWWVSPLLGVRVAHPGVFNFGALALSLLGAVGLLALLSVVRRRPGGDAR"
     gene            1021..2463
                     /locus_tag="VF453_03300"
     CDS             1021..2463
                     /locus_tag="VF453_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7686703.1"
                     /translation="MTHALIVEDDADSADTMAELIAAQGFTVAVARNLRDARRQIILQ
                     LPDLVLLDLQLPDGSGMELFQDESLVANSEIVLITGHASLDSSIQALRMGAADYLVKP
                     VNLKQLQGILSRVMRPSALQAELASLTAEWESNGHFGHLWGRSPPMRRIYEQISRVAV
                     TAVSVFITGESGTGKEVVAQTVHDLSRRRKHPFLAVNCGAISPNLIESEIFGHEKGSF
                     TGADRQHQGFFERAHGGTLFLDEITEMPLELQVKLLRVLETGLFMRVGSTVAQETDVR
                     VIAATNRPPELAVAQGKLREDLLYRLNVFPIELPPLRDRSEDVPLLAQHFLSAICERE
                     GTVKSFTPAAIQRLVGYRWPGNVRELRNVVQRAYVMAPSVQITEEWLPEDPAGAASSS
                     VAAASPSASTTSGARAASPASAAAAPAGPTITIPIGTSMAEAERRLILATLEHFHQQK
                     ERTAAALGVSLKTLYNRLKEYSQDKVDTAE"
BASE COUNT          383 a          938 c          892 g          351 t
ORIGIN      
        1 tcgtcgccgg cgcgctcggt ctcgtcgatc gggtcgtcgt cgtcggcgcc gcgggcgggc
       61 gcgccggcct cgatgtcgtc gtccgcgccg atgccggcga tgtcgggatc gccgtcggcg
      121 acgcgttccg cctcgcggtc gtcgtcgcga cgcgcctgct cgggatcgcg ctcgccctcg
      181 acgaggtccc gcgcggcgtc gaggtagacg tcgcccgcct tgtcgggacg cggctggtcg
      241 gagcgctcgc cgggcaggtc ggcgcgctcc tggcgggcca tcgccgcgtc gacgccgcgg
      301 tcggtggagc gatccatggc gtccagcggg tcggccgccg gtcggagggt gggtgcgggc
      361 atggcgatct ccgatagagg tgggatgccg cggttcagca accgctatgc ccagcgtcgc
      421 gtcagcgggc gtcgccgccc ggacggcggc ggacgacgct cagcagggcc agcagcccga
      481 ccgcccccag cagcgacagc gccagcgcgc caaagttgaa gacgccagga tgcgccaccc
      541 gcacgcccag cagcggcgag acccaccagc cggcagccag cgccccgacg atgcccaccg
      601 ccacgttcgg gaagacggcg gcgtcctcct cgtggcgcat gagcaacccc gcgacggcgc
      661 cgacgaccat ccccacgacg agccagacgg caatgttgag caggttgagc atggcggtcg
      721 cctcgtgtcg atgagtctcc ggcccacggc cgggctggag ctacagcaat cggcgtgccg
      781 gtaggcgcgc atcccgatgc ggtcgagctc tcgtctgaca agccgtgacc ggcatcctgc
      841 gcaaaatggc tctcgactcg gcgtatgctg cgcgtcgcgg gtcgctcgac catgtggggc
      901 gaccgcgcaa tccggacctg gaagggtcgt gtccggcgtg agaggcgcac aagacgctcg
      961 agtcgcttcc agccgccttc gggcgctttc gaagaaccaa gatccggaca cgaacccatc
     1021 atgacccatg cgttgatcgt cgaggacgac gccgattccg ccgacacgat ggcggagctc
     1081 atcgccgccc agggcttcac cgtggcagtg gcgcgcaacc tgcgcgacgc gcgccgccag
     1141 atcatcctgc aactgcccga cctggtgctg ctcgatctcc agctgcccga cggcagcggg
     1201 atggagctgt tccaggacga gagcctggtg gccaactcgg agatcgtgct gatcacgggg
     1261 catgcgagcc tcgacagctc catccaggcg ttgcgcatgg gcgccgccga ctacctggtc
     1321 aagccggtca acctgaagca gctgcagggc atcctgtcgc gcgtgatgcg gccgtcggcc
     1381 ctgcaggccg agctcgcgtc gctcaccgcc gaatgggaga gcaacggcca cttcggccac
     1441 ctctggggcc gctcgccgcc gatgcgccgc atctacgagc agatctcgcg cgtggcggtg
     1501 acggcggtgt ccgtcttcat cacgggcgag agcggcaccg gcaaggaggt cgtcgcgcag
     1561 accgtgcacg acctgagccg ccgccgcaag catcccttcc tcgccgtgaa ctgcggcgcg
     1621 atctcgccca acctcatcga gagcgagatc ttcggccacg agaaaggctc cttcacgggc
     1681 gccgaccgcc agcaccaggg cttcttcgag cgcgcccacg gcggcacgct gttcctcgac
     1741 gagatcacgg agatgccgct cgagctgcag gtcaagctgc tgcgcgtgct ggagacgggg
     1801 ctgttcatgc gcgtcggctc gaccgtcgcg caggagaccg acgtgcgcgt catcgccgcc
     1861 accaaccggc cgcccgagct cgccgtcgcc cagggcaagc tgcgcgagga cctgctctat
     1921 cgcctcaacg tcttcccgat cgagctgccg ccgctgcgcg accgctcgga ggacgtgccg
     1981 ctgctcgccc agcacttcct gtcggcgatc tgcgagcgag aaggcaccgt caagagcttc
     2041 acgccggcgg cgatacagcg cctggtcggc taccgctggc cgggcaacgt ccgcgagctg
     2101 cgcaacgtcg tccagcgcgc ctacgtgatg gcgccgtcgg tgcagatcac cgaggaatgg
     2161 ctgccggagg atccggccgg cgcggcgtcc agctcggtcg cggcggcctc gccgtcggcg
     2221 tcgaccacct cgggcgcgcg cgccgcgagc ccggcctcgg ccgccgcggc gccggcgggc
     2281 ccgacgatca ccatcccgat cggcacctcc atggccgagg cggagcgacg cctgatcctc
     2341 gccacgctgg agcacttcca tcagcagaag gaacgcaccg cggcggcgct gggcgtcagc
     2401 ctgaagacgc tctacaaccg gctcaaggaa tacagccagg acaaggtcga cacggcggaa
     2461 tgagccgcgg ccccgcgcgc ccgcggggcc ctcggccgcg tgtcgatcag cgcttgatct
     2521 ggttgccgac gatgccgccc acggcggcgc cgccgaccgc gccg
//
LOCUS       DASVLN010000145         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_276582, whole genome shotgun sequence.
ACCESSION   DASVLN010000145 DASVLN010000000
VERSION     DASVLN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276582"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..415
                     /locus_tag="VF453_03305"
     CDS             50..415
                     /locus_tag="VF453_03305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7686704.1"
                     /translation="MAAAAKEPGASVAADGLVYRSLKDGTGASPAATDIVKVNYRGTF
                     VDGEEFDSSYSHGQPISFPLNRVIPCWTEGVQKMKVGGKAKLTCPSAIAYGSRGAGGV
                     IPPDATLVFEVELLGINGK"
     gene            723..857
                     /locus_tag="VF453_03310"
     CDS             723..857
                     /locus_tag="VF453_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686705.1"
                     /translation="MVGVGLLSQVASAGVADGSFAAMVVVLALAAIATGLRWIPKEGR
                     "
     gene            complement(862..1176)
                     /locus_tag="VF453_03315"
     CDS             complement(862..1176)
                     /locus_tag="VF453_03315"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX7686706.1"
                     /translation="MADTGSPLPSPLRTRIESLSGMDMSAVRVHYNSPRPAQLGAQAH
                     AQGTDIHLAPGQGHRLAHEAWHIVQQRQGRVGDNLRTHVGLSTDPHAREVECDMLARR
                     TP"
     gene            complement(1217..1987)
                     /locus_tag="VF453_03320"
     CDS             complement(1217..1987)
                     /locus_tag="VF453_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006755859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX7686707.1"
                     /translation="MRTVVFNQKGGVGKSTITCNLAAISAHQGLRTLVVDLDPQGNST
                     RYLLGDLADDAESLPSVAEFFEQTLKFVARTRGAGEFIVETPFEHLHLMPSAPALEEL
                     HAKLESRYKIYKLRDALDEIAGDYDRVYIDTPPAMNFYTRSALIAAGGCLIPFDCDDF
                     SRRALYALLDNVKEIQADHNEGLAVRGIVVNQFQPRANLPQRLVQELVDEGLPVLEPY
                     LSSSVKVRESHQLARPMIHLDPRHKLTQELVALHERLG"
     gene            complement(2096..>2371)
                     /locus_tag="VF453_03325"
     CDS             complement(2096..>2371)
                     /locus_tag="VF453_03325"
                     /inference="COORDINATES: protein motif:HMM:NF028480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7686708.1"
                     /translation="KIARDLRGQGLGRHDVATIGAKAAADLDAVAATLGEAPFFGGAE
                     PSALDACAYAFLANLIRVPVDSPVKAHALGLAPLVAYDARMRARVGA"
BASE COUNT          296 a          831 c          878 g          366 t
ORIGIN      
        1 cttcgccgcc ggtgccgcga ccgccgccct cgccgacgac cacgccgccc tggccgccgc
       61 cgccaaggag cccggcgcca gcgtcgccgc ggacggcctg gtctaccgtt cgctcaagga
      121 cggcaccggc gccagcccgg cggcgaccga catcgtcaag gtcaactacc gcggcacctt
      181 cgtcgacggc gaggagttcg acagctcgta cagccacggc cagccgatct ctttcccgct
      241 caaccgcgtc atcccgtgct ggaccgaggg cgtccagaag atgaaggtcg gcggcaaggc
      301 caagctgacc tgcccgtccg cgatcgccta cggctcgcgc ggcgccggcg gcgtgatccc
      361 gccggacgcg acgctcgtgt tcgaggtcga gctgctcggc atcaacggca agtgagcggc
      421 ggccggcccg gcggctcgcc ggggcacggc gcttgcagcg ggcggcgtca tgccgtcgtt
      481 cgcccgttcc gatcccgccg aagcggcgcc cccgcccgcc gtgcgcggcc cttggccggc
      541 gcgcctggcg gcgctggacc tcgggctcat gttcatggcc agcacgctcg tcacgccgct
      601 gtacgggccg tggcgcgagg cgttcgggtt ctcggcgctc acgctgactc gcgcccgatg
      661 cgcggcgcgc cgaggtcgtc tcgtgcttcc tgctcgcctg cttcctcggc aactcgctgc
      721 cggtggtcgg cgtcgggttg ctgtcgcagg tcgcctcggc cggcgtggcc gacggctcgt
      781 tcgcggcgat ggtcgtcgtg ctggcgctgg cggcgatcgc gaccgggctg cgctggatcc
      841 cgaaggaagg acgctagcgg gctagggcgt gcgccgcgcg agcatgtcgc actcgacctc
      901 gcgcgcgtgc ggatcggtcg acaacccgac atgcgtgcgc aggttgtcgc cgacgcgccc
      961 ctggcgctgc tgcacgatgt gccaggcctc gtgcgccagc cgatggccct ggcccggggc
     1021 gaggtggatg tcggtgccct gggcatgggc ctgcgcaccg agctgcgccg ggcgcggcga
     1081 gttgtagtgc acgcgcacgg cgctcatgtc catgccggac aggctctcga tgcgggtcct
     1141 caagggcgag ggcagtgggc ttccggtgtc ggccatggcg cgtcctgtcg ggctggggtg
     1201 cccgattgtg cgccggtcag ccgagccgtt cgtgcagcgc caccagctcc tgcgtgagct
     1261 tgtgccgcgg atcgaggtga atcatcggcc gcgcgagctg gtgcgactcc ctgaccttga
     1321 ccgagctcga gaggtacggc tcgagcacgg gcaggccctc gtcgaccagc tcctgcacga
     1381 gccgctgcgg caggttggcg cggggctgga actggttgac gacgatgccc cgcaccgcca
     1441 ggccctcgtt gtggtcggcc tggatctcct tgacgttgtc cagcagcgcg tagagcgcgc
     1501 ggcgcgagaa gtcgtcgcag tcgaacggga tcaggcagcc gcccgccgcg atcagcgccg
     1561 accgcgtgta gaagttcatt gccggcggcg tgtcgatgta gacgcggtcg tagtcgcccg
     1621 cgatctcgtc gagcgcgtcg cgcagcttgt agatcttgta gcgcgactcg agcttggcgt
     1681 gcagctcctc cagcgcgggc gcggacggca tcaggtgcag gtgctcgaac ggcgtctcga
     1741 cgatgaactc gccggcgccg cgcgtgcggg cgacgaactt cagcgtctgc tcgaagaact
     1801 ccgcgacgct cggcagggac tcggcgtcgt cggccaggtc gccgagcagg tagcgcgtcg
     1861 agttgccttg cgggtcgagg tccacgacca gcgtgcgcag cccctggtgc gcgctgatgg
     1921 ccgcgaggtt gcaggtgatc gtggacttgc ccacgccgcc cttctggttg aacacgacgg
     1981 tgcgcatgac gactggcctc gcggatgcgg ttggatgcga agtcgccagt ctagcgcggc
     2041 gttgctgcag cgcagcatca aattttggag gcggcggccg agttccgccg cagcctcatg
     2101 cgccgacgcg cgcccgcatc cgcgcgtcgt aggccaccag cggcgcgaga cccagggcgt
     2161 gcgccttcac ggggctgtcg acgggcacgc ggatcaggtt ggcgaggaac gcgtaggcgc
     2221 aggcgtcgag cgccgacggc tcggcgccgc cgaagaacgg ggcctcgccg agcgtcgcgg
     2281 cgacggcgtc gaggtcggcg gcggccttgg cgccgatcgt cgcgacgtcg tgccgaccca
     2341 ggccctgccc ccgcaggtcg cgcgcgatct t
//
LOCUS       DASVLN010000146         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_329708, whole genome shotgun sequence.
ACCESSION   DASVLN010000146 DASVLN010000000
VERSION     DASVLN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329708"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1367)
                     /gene="fabF"
                     /locus_tag="VF453_03330"
     CDS             complement(123..1367)
                     /gene="fabF"
                     /locus_tag="VF453_03330"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7686709.1"
                     /translation="MSRRRVVVTGLGLVCPVGNTVAEGWANIVAGKSGIATITKFDPT
                     PFACKFAGEVKGFNVEDYIPGKDARHMDTFIHFGVAAAMQAIKDAGLPTGDQCPEELA
                     DRIGVLVGAGIGGLPEIERTQMELANRGPRRVSPFFVPASIINMISGHVSIYCGYRGP
                     NLGLVTACTTGLHSIGVSARLIEYGDADIMVAGGAESTVSPLGIGGFAAARALSTRND
                     DPAGASRPWDKDRDGFVLGEGAGVLVLEEYEHAKRRGAQIYAELSGFGMSGDAYHMTA
                     PNVDGPRRSMKAALDNAGINADQVQYLNAHGTSTPLGDLNETNAVKLAFGDHAKKFVV
                     SSTKSMTGHLLGGAGGIESVFTVLALHHQVVPPTINLVNQDPECDLDYCANTAREMKL
                     DVAVKNNFGFGGTNGTLVFRRV"
     gene            complement(1568..1807)
                     /gene="acpP"
                     /locus_tag="VF453_03335"
     CDS             complement(1568..1807)
                     /gene="acpP"
                     /locus_tag="VF453_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7686710.1"
                     /translation="MSDIEARVKKIIAEQLGVPEADVANEKAFVADLGADSLDTVELV
                     MALEDEFSIEIPDEEAEKITTVQLAIDYAKAHAKG"
     gene            complement(1904..2650)
                     /gene="fabG"
                     /locus_tag="VF453_03340"
     CDS             complement(1904..2650)
                     /gene="fabG"
                     /locus_tag="VF453_03340"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX7686711.1"
                     /translation="MSDATTVQTALVTGASRGIGRAIALALAQRGYRVIGTATSDAGA
                     QAITEALAGHTGCKGVKLDVNDPAGVTAVVEGIVKEYGGLQVLVNNAGITRDGLAMRM
                     KDEDWDAVHDTNLKAVFRMTREVMRTMMKQRYGRIVNITSVVGASGNPGQANYAAAKA
                     GVAGMTRALARELGSRNITVNCVAPGFIETDMTHVLPEAQKAALLQQIPANRLGRPEE
                     IAHAVAFLASPEAGYVTGVELHVNGGMYMN"
     gene            complement(2647..>3228)
                     /locus_tag="VF453_03345"
     CDS             complement(2647..>3228)
                     /locus_tag="VF453_03345"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7686712.1"
                     /translation="MQEAVPVGTGGMAAILGLDAQAVRDGCAAAAAASGEVVEAVNFN
                     DPKQTVIAGSKAGVEKACELLKAAGAKRALPLPVSAPFHSSLMRPAAERLKEALAAVT
                     LHAPAIPVVNNVDVATPADADAIRDALVRQAAGAVRWVEVVQALRARGLTHVIECGPG
                     KVLAGMVKRIDGELVSASIYDPATLAEAKGMLE"
BASE COUNT          440 a         1128 c         1144 g          516 t
ORIGIN      
        1 gcgcagcgac cgacgccgcg acgacgagcg ccagcgcgcc gcgccaggcg cggccgagcg
       61 agctggtgac ctcgacgggg ggcggcgcgc gcattcgctc ggcggggagc cggctgccgc
      121 ggtcagacgc gacggaacac cagggttccg ttggtgccgc cgaagccgaa gttgttcttc
      181 acggcgacgt cgagcttcat ctcccgcgcc gtgttggcgc agtagtcgag gtcgcactcg
      241 gggtcctggt tgaccaggtt gatggtcggc gggacgacct ggtggtgcag cgcgagcacg
      301 gtgaacaccg actcgatgcc gccggcgccg cccagcaggt ggccggtcat cgacttcgtc
      361 gagctgacga cgaacttctt cgcgtggtcg ccgaaggcca gcttcaccgc gttggtctcg
      421 ttcaggtcgc ccagcggcgt cgacgtgccg tgcgcgttca ggtactgcac ctggtcggcg
      481 ttgatgcccg cgttgtccag cgccgccttc atcgagcggc gggggccgtc gacgttgggc
      541 gcggtcatgt ggtacgcgtc gccgctcatg ccgaagcccg agagctccgc gtagatctgc
      601 gcgccacggc gcttggcgtg ctcgtactcc tcgagcacga gcacgcccgc gccctcgccc
      661 agcacgaagc cgtcgcgatc cttgtcccag gggcgcgagg cgccggccgg atcgtcgttg
      721 cgggtcgaca gcgcgcgcgc cgccgcgaag ccgccgatgc ccaggggcga caccgtcgat
      781 tccgcgccgc cggcgaccat gatgtcggcg tcgccgtact cgatcaggcg ggcggacacg
      841 ccgatcgagt gcaggcccgt cgtgcaggcg gtcaccaggc ccaggttggg cccgcggtag
      901 ccgcagtaga tcgagacgtg gccggagatc atgttgatga tggaggccgg cacgaagaac
      961 ggcgacacgc gacgcgggcc gcggttggcg agctccatct gcgtgcgctc gatctccggc
     1021 aggccgccga tgccggcgcc caccagcacg ccgatgcggt cggcgagttc ctcagggcac
     1081 tgatcgcccg tcggcagccc ggcgtccttg atcgcctgca tcgccgccgc cacgccgaaa
     1141 tggatgaagg tatccatgtg gcgagcgtcc ttgccgggga tgtagtcctc gacgttgaag
     1201 cccttgacct cgcccgcgaa cttgcaggcg aacggcgtgg ggtcgaactt ggtgatggtg
     1261 gcaatgcccg acttgccggc cacgatgttg gcccagccct cggccaccgt gttgcccacg
     1321 ggacagacca gacccagtcc ggtgacgacg acgcgacgac gactcataga ttcactcgat
     1381 caatgctcca cacggagctt cacgctgacg cagcaatcgc gggaccgccg aacgaaggtg
     1441 tccgacgtcc ccggcgctgc cgctcacacg tcccaaacgg aaaaccgggg gagggaggga
     1501 tctggctccg ccagtcccga tccggccggc cccctcgggg ggcagcgacc gtcagggagc
     1561 gtgggggtca acctttcgca tgggccttgg cgtagtcgat cgccagctgc accgtcgtga
     1621 tcttctcggc ttcctcgtcg gggatctcga tgctgaactc gtcctcgagg gccatcacga
     1681 gttcgaccgt gtcgagcgag tcggcgccca ggtcggcgac gaaggccttc tcgttggcca
     1741 cgtcggcttc ggggacgccg agttgctcgg caatgatctt cttcactcgt gcttcgatat
     1801 cgctcatgac actcctccgg gaggtttgcg taaaaacggg cggattctac ttgcccggaa
     1861 aaccggtccg gcgcgtcgac cccagggccg ggcgccggcc cgctcaattc atgtacatgc
     1921 cgccgttgac gtgcagctcg acgccggtga cgtagcccgc ctcgggcgag gccaggaacg
     1981 cgacggcatg cgcgatctcc tcgggccggc cgaggcggtt cgcggggatc tgctgcagca
     2041 gcgcggcctt ctgggcctcg ggcaggacgt gcgtcatgtc ggtctcgatg aagcccggcg
     2101 cgacgcagtt gaccgtgatg ttgcggctgc ccagctcgcg cgccagggca cgcgtcatgc
     2161 ccgcgacgcc ggccttggcc gccgcgtagt tggcctgccc ggggttgccg ctggcgccca
     2221 ccaccgaggt gatgttgacg atgcggccgt agcgctgctt catcatcgtg cgcatgacct
     2281 cgcgcgtcat gcggaagacg gccttcaggt tggtgtcgtg caccgcgtcc cagtcctcgt
     2341 ccttcatgcg catggccagg ccgtcgcgcg tgatgccggc gttgttgacc agcacctgca
     2401 ggccgccgta ctccttgacg atgccctcca cgacggccgt gacgccggcg ggatcgttga
     2461 cgtcgagctt cacgcccttg cagccggtgt ggccggccag ggcctcggtg atcgcctggg
     2521 cgccggcgtc gctcgtcgcg gtaccgatca cgcggtagcc gcgctgcgcg agcgcgagcg
     2581 cgatggcgcg gccgatgccg cgcgaggcgc cggtcaccag cgcggtctgg acggtcgtcg
     2641 cgtcgctcat tccagcatcc ccttcgcttc ggccagcgtg gccgggtcgt agatcgaggc
     2701 cgagaccagc tcgccgtcga tgcgcttgac catgccggcc aggaccttgc ccgggccgca
     2761 ttcgatgacg tgcgtcaggc cgcgggcgcg cagcgcctgc acgacctcca cccagcgcac
     2821 cgcgcccgcc gcctggcgca cgagcgcgtc gcggatggcg tcggcgtcgg ccggggtggc
     2881 gacgtccacg ttgttgacga ccgggatcgc gggcgcgtgg agcgtgacgg cggccagggc
     2941 ctccttcagg cgctcggccg cggggcgcat caggctcgag tggaacggcg ccgacaccgg
     3001 cagcggcagc gcgcgcttgg cacccgcggc cttgagcagc tcgcaggcct tctcgacgcc
     3061 tgccttgctg ccggcgatga cggtctgctt cgggtcgttg aagttcaccg cctcgacgac
     3121 ctcgccgctc gccgcggccg cggcggcgca gccgtcgcgc acggcctgcg cgtccaggcc
     3181 gaggatcgcg gccatgccgc cggtgcccac gggcacggcc tcctgcat
//
LOCUS       DASVLN010000147        13798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_60965, whole genome shotgun sequence.
ACCESSION   DASVLN010000147 DASVLN010000000
VERSION     DASVLN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60965"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VF453_03350"
     CDS             complement(<1..460)
                     /locus_tag="VF453_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7686713.1"
                     /translation="MTPYLDIPVTEASQVGDARRNATRLAASRGLDETACGRIALVVT
                     ELGTNLVRHARRGRLLLGCAAGADGCRFEAISIDHGPGMADVARCLRDGYSSGGTAGT
                     GLGAVRRLSSGFSIHSQPDKGTVVLSRVWIPAAEAGAGDAGARSRVAVAGV"
     gene            complement(462..866)
                     /locus_tag="VF453_03355"
     CDS             complement(462..866)
                     /locus_tag="VF453_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX7686714.1"
                     /translation="MSEPLTGAMPLASERDIVMSRQQVRKLTQQAKFSLVDQTKMITA
                     ASELSRNTVVYGGGGEMRWEILTEGIRTGVRLWFEDKGPGIPDVDLALTDGWTSGSGM
                     GVGLSGSRRLVNEFELRTVVGEGTCVTVTRWR"
     gene            complement(859..1233)
                     /locus_tag="VF453_03360"
     CDS             complement(859..1233)
                     /locus_tag="VF453_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7686715.1"
                     /translation="MERIPILRMGRNLLVTIQVDLQDQSALALQDDLAARIEATGADG
                     VLIDISALEIVDSFIGRMLASISGIARVLDATTVVVGMQPAVAITLVELGLSLEGVRT
                     ALDVERGMALLEAAHAERGRLE"
     gene            complement(1241..2101)
                     /locus_tag="VF453_03365"
     CDS             complement(1241..2101)
                     /locus_tag="VF453_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7686716.1"
                     /translation="MNIETKALLEAVTADQERFQQEWLSEIASSGTAGAASSTATQDV
                     RDMVIALVEALRSDADLERFNDAPGFGRLRNLLGGLSASRAAQGSSAAETSHFVMALK
                     RPLFTGLKASRGQDPQGLFDAMWSASLLVDKMAQWTTNTYQRSREEVIQRQQEELLEL
                     STPVVKLWEGVLAVPMIGTLDSGRTQLVMESLLQRIVETGSELAIIDITGVPTVDTLV
                     AQHLLKTVTAIRLMGADCIISGIRPQIAQTIVHLGIDLQGITTKATLADALQLAMKRA
                     GYVVTKAAKA"
     gene            2444..3370
                     /gene="rpoH"
                     /locus_tag="VF453_03370"
     CDS             2444..3370
                     /gene="rpoH"
                     /locus_tag="VF453_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX7686717.1"
                     /translation="MTTTQATALSVRDPWALVPPLGNLDAYISAINRLPMLSQEEETR
                     LATKLRDEGDLHAASALVMSHLRLVVSVSRQYLGYGLPHGDLIQEGNIGLMKAVKRFD
                     PDQGARLVSYAMHWIKAEIHEYILKNWRMVKVATTKAQRKLFFNLRSLKSKLKDEASD
                     ADVHRNTLTRAQVERMASDLNVKPEEVLEMETRMSGGDIAMESQNDDGEETFAPIAYL
                     ADDRDEPTRVIEKAAHDRLAGQGIVEALDQLDPRSRRIVEERYLKVNDDASGGMTLHE
                     LAAEYGVSAERIRQIEVAALKKMRKTLEPQRA"
     gene            complement(3445..4083)
                     /locus_tag="VF453_03375"
     CDS             complement(3445..4083)
                     /locus_tag="VF453_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7686718.1"
                     /translation="MTSTRRLLLAAALPLALGLAVLAGCDRGPAAPPPPPPHKFAAID
                     LTGAEYARQFSLPDFDGKVRTLADFKGKVTAVFFGYTQCPDACPTTMAELAGVAKTLG
                     PDASRLQVVFITVDPARDTPGLLKAYVTNFRPDFLALRGDDAQTREVAKEFKVFYEKV
                     PGKTPETYTMDHTAGVYIFDPQGRIRLFARQNMDPGQLAGDIQALLAQSHAA"
     gene            complement(4095..5006)
                     /gene="cyoE"
                     /locus_tag="VF453_03380"
     CDS             complement(4095..5006)
                     /gene="cyoE"
                     /locus_tag="VF453_03380"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX7686719.1"
                     /translation="MSTAVPIPSSAKQPAPPSRWRQFYALTKPRVVQLIVFCAVIGMF
                     LAVPGWPPLVPALAATAGIWLVAAAAAAFNCLVEQEIDRRMARTAWRPTARGELTTAQ
                     TLVFSGLLCGAGAVLLLAWVNALTLWLTLATFVGYAIVYTLLLKPRTPQNIVIGGASG
                     AMPPVLGWAAMTGHADANAWLLCLIIFLWTPPHFWALALYRTEDYRKAGLPMLPVTHG
                     PDFTKLHVFLYTIVLLAGTMLPFVSGLSGLPYLAAALVLGLVFCWYGFRLWRDYSDAL
                     ARKTFRFSILYLSLLFAALLVDHYIPR"
     gene            complement(5003..6106)
                     /locus_tag="VF453_03385"
     CDS             complement(5003..6106)
                     /locus_tag="VF453_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX7686720.1"
                     /translation="MNATPAYDWHAAPGLALIALGIVSIVLMLAWWRGGLRRLGRGQR
                     VAALAGLTLFLCFDLVCFGAYTRLSDSGLGCPDWPGCYATASPVAAAHPIAEAEARAP
                     GGPVTERKAWIEMIHRYFATALGVLIIGLVAAAALARDRRALAWALVTLAWVIGQGVF
                     GALTVTWKLYPAIVTGHLLGGMGLLALLALQAPAPAVGGLVRARDRAALAIASVALLV
                     QLALGGWVSTNYAVLACSGFPTCNGQWWPRMDWAEGFTLARELGRRADGAPIAMSALV
                     AIQWMHRLFALALTAALAWLVVRLSRRGREARRRAGWILAILVVQWGTGLSNVVLGWP
                     MAAALAHTAGAAALVICLGREAAALLKSRITPS"
     gene            complement(6103..6924)
                     /locus_tag="VF453_03390"
     CDS             complement(6103..6924)
                     /locus_tag="VF453_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686721.1"
                     /translation="MPDPTPTAPTLAPTDPTQPVPLEPLAASSHEVHADDEDVPAAAR
                     LAADGLESLSFTVHDTPEPVRDAQDARSGRGRRTMWAILLLCALPVVLSYFTYYVIRP
                     QGRANYATLIEPQRPLPGLDRVPAQTLDGRSAPLTALKGQWLLVVVAPAGCGAGCEKL
                     LFMQRQLREMVGAERDRIDKVWLVDSAGPVAPTLLSAVEAPPAVIVRRVDRDALARWL
                     EPEPHHALEEHLYIVDPLGNWMMRAPVDADPVKLKRDIDRLLRASSWWDRPKDGR"
     gene            complement(6917..7771)
                     /locus_tag="VF453_03395"
     CDS             complement(6917..7771)
                     /locus_tag="VF453_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX7686722.1"
                     /translation="MTDDYSTRDPARSAGSPGPHGVAGNAEGASVERRRGPTAASRLG
                     WLVVAVMCAATTARLGVWQLSRAHQKESAAALVAERSHEPPLPAAALATDAAGAAAQW
                     QRRIELEGRWDVAHTVFLMNRTMDARSGFYVMTPLKLPSGDAVVVQRGWIARDDARPM
                     AAPPVPAPGGTVRVAGHVAPWPAHWIELGKPVPGPVRQNLEPGPFAAEAGLALRPEIV
                     VEDEGPGAPSDGLRRDWPAPDIGTATNYGYAAQWFAMSVAFLGLWAWTQFFRRRAGAS
                     SASDPTDA"
     gene            7770..7991
                     /locus_tag="VF453_03400"
     CDS             7770..7991
                     /locus_tag="VF453_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2909 domain-containing protein"
                     /protein_id="HEX7686723.1"
                     /translation="MRIVIVGFLVLVLAALGGAGFFMLRRKPDDGRADKRMARALAVR
                     VGLSVTLFLLILLAYALGWIQPRGIPVGT"
     gene            complement(8124..9008)
                     /locus_tag="VF453_03405"
     CDS             complement(8124..9008)
                     /locus_tag="VF453_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7686724.1"
                     /translation="MSAATPHGSTPHYFIPAPSRYPVTLAAGMLLVIFGGAQWINDNG
                     WGKWVLLAGLVVWLSTLAQWFSQAIGESEGGQYSDRVDTSFRWSMSWFIFSEVMFFAS
                     FFGALFWARNIALPMLGNLDHQLLWPDFHAVWPSLQAGATASPADTIAAFHTMGPWPI
                     PTLNTAILLSSGVTLTIAHHALIANHRAKAVGWMWITVLLGASFLCCQAYEYHEAYTA
                     MNLKLSSGIYGSTFFMLTGFHGFHVFVGMLMLFFITMRLQKGHFTPTRHFGFEGAAWY
                     WHFVDVVWLGLYVVVYWL"
     gene            complement(9059..9322)
                     /locus_tag="VF453_03410"
     CDS             complement(9059..9322)
                     /locus_tag="VF453_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEX7686725.1"
                     /translation="MPEPVPPADEPERDPHSPLARKAAFGQTFRAVAWSFLGIRRSKD
                     HEQDVKRLNPLHVVIAALLAAALFVLALIALVHFVIGLSGASH"
     gene            complement(9331..9960)
                     /locus_tag="VF453_03415"
     CDS             complement(9331..9960)
                     /locus_tag="VF453_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX7686726.1"
                     /translation="MTTSTTPANPRAAARPAALRRHNRRMLVGLTVFVVLMVGFGYAM
                     VPMYRSICEALGVNVIARDDGDGQVDAGAANSQVDLSRSVTIEFDANVRGPWEFHPEQ
                     SSVVVHPGALTTVMYEFKNTQSRTMTAQAIPSYAPGGAQPHFNKVQCFCFTQHTLAPG
                     ESKRWPVTFFVDDKLPKDIKTITLSYSFFEVGGTMPAAPSGDAPPAPHA"
     gene            complement(9974..10090)
                     /locus_tag="VF453_03420"
     CDS             complement(9974..10090)
                     /locus_tag="VF453_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HEX7686727.1"
                     /translation="MALTPEQRRKNLRLGLVLASVAFVFLAGFVAKAILFGL"
     gene            complement(10114..11742)
                     /gene="ctaD"
                     /locus_tag="VF453_03425"
     CDS             complement(10114..11742)
                     /gene="ctaD"
                     /locus_tag="VF453_03425"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX7686728.1"
                     /translation="MSAVLPHPIDHGHEDDHAHGHHPTGWRRWLFATNHKDIGTLYLL
                     FSFTMFLIGGILALCIRLELFRPGLQYFNPELFNQFTTMHGLIMVFGAIMPAFVGFAN
                     WMIPMQIGAADMAFARMNNLSFWLLIPAALMLAGGFFMPGGAPAAGWTLYAPLTLQMG
                     MSMDSSIFALHIMGASSIMGSINIIVTILNMRAPGMTLMKMPMFAWTWLITAYLLIAV
                     MPVLAGAITMTLTDRHFHTSFFNPAGGGDPVMYQHVFWFFGHPEVYIMILPAFGIISQ
                     VVPAFARKRLFGYTSMVYATASIAILSFIVWAHHMYTTGMPVTGQLFFMYATMLISVP
                     TGVKVFNWLATMWRGSMTFEAPMLWAVGFIFVFTMGGFTGLICSMAPIDIQLQDTYYV
                     VAHFHYVLVAGSLFGMFSGFYYWSPKWTGVMYNETRGKIHFWASLITFNVTFFPMHFL
                     GLAGMPRRIADYPMQFADFNALASIGGLGFGLSQVYFLLFVVIPVMRGKGEPAPQRPW
                     EGAEGLEWEVPSPAPWHTFETPPKLDKTATKVIG"
     gene            complement(11803..13005)
                     /gene="coxB"
                     /locus_tag="VF453_03430"
     CDS             complement(11803..13005)
                     /gene="coxB"
                     /locus_tag="VF453_03430"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX7686729.1"
                     /translation="MTTCSPRQLPLRAALAALASTAAGLAAAAPNADGVTDLPGGPRL
                     NGVDFQPAVTDIAQQLKDLHYGMLVVCTAIFVVVFGFMFYSIVKHRKSVGHQAANFHE
                     SVAVEIAWTVVPFVIVILMGAFATRTVVAMKDTTNPDLTIKVTGYQWKWGYDYVKGEG
                     EGLSFISTLDTAQRELSEEDKAAGNDYLLKVDNPLVVPVDRKVRVITTANDVIHSWSV
                     PAFGVKQDAIPGFVRDTWFRATKPGDYYGMCSELCGKEHAYMPIHVIVKSAADYTAWV
                     AAQRKALAAAADDPKKVWEMGPLVARGEKVYNANCGVCHQANGKGGGAVKALDGSPVV
                     NDADKNAQILTVLHGRKVNGVEAMPAWQGKLSETDIAAAITYTKNSWSNHTGQVVQPA
                     DVLADESK"
     gene            complement(13169..>13798)
                     /locus_tag="VF453_03435"
     CDS             complement(13169..>13798)
                     /locus_tag="VF453_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HEX7686730.1"
                     /translation="AAPAWRRWLGLAAAGPRAMPQSAVPAGQAQLVWSNMALHWAPDA
                     PTLMRDWHAALAVDGFLMFTTVGPGTLPELAEAYRRRGWGTPYAPWTDMHDLGDMLVE
                     AGFADPVMDQETLRLTWSSPQAALDELHALGANTDRARAAGLRTPRWREALLEALGER
                     RDRDGRVVLEFEIVYGHAFKPQPRAKVAARTEVGLEDMKSMLSRGRPAR"
BASE COUNT         2212 a         4733 c         4902 g         1951 t
ORIGIN      
        1 tcacccccgc gaccgcgacg cgcgaccgcg cgccggcgtc gcccgcaccg gcctcggccg
       61 ccgggatcca gacgcgcgag aggacgaccg tgcccttgtc cggctgcgag tggatcgaga
      121 agccgctcga caggcgccgc accgcaccga gcccggtgcc ggcggtgccg ccgctcgagt
      181 agccgtcgcg caggcagcgc gcgacgtcgg ccatgcccgg gccgtggtcg atcgagatcg
      241 cctcgaagcg gcagccgtcg gcgcctgcgg cacatcccag cagcaggcgt ccgcggcggg
      301 cgtggcggac caggttcgtg ccgagctccg tcacgaccag cgcgatgcgg ccgcaggcgg
      361 tctcgtccag gccgcgcgag gcggcgagcc gggtcgcgtt gcggcgggcg tcgccgacct
      421 gcgaggcctc ggtgaccggg atgtccaggt acggggtcac gctatctcca gcgcgtgacg
      481 gtgacgcagg ttccctcgcc gacgacagtg cgcagctcga attcgttgac cagccgccgg
      541 ctgcccgaca ggccgacgcc catgccgctg cccgaggtcc agccgtcggt cagcgcgaga
      601 tccacgtcag ggatgcccgg gcccttgtcc tcgaaccaca gccgcacgcc ggtgcggatg
      661 ccttcggtca ggatctccca gcgcatctcg ccgccgccgc catagaccac cgtgttgcgc
      721 gagagctcgc tcgcggccgt gatcatcttg gtctggtcga cgagcgagaa cttcgcctgc
      781 tgcgtcagct tgcgcacctg ctggcggctc atgacgatgt cgcgctcgct cgcgagcggc
      841 atcgcgccgg tcagcggctc actcaaggcg accccgctcg gcgtgcgcgg cctccagcag
      901 ggccatgccg cgctcgacgt ccagcgccgt gcgcacgccc tccagcgaca gcccgagctc
      961 gaccagcgtg atggccaccg ccggctgcat gccgacgacg accgtggtcg cgtccagcac
     1021 ccgggcgatg ccggagatgc tcgccagcat gcggccgatg aaggagtcga cgatctccag
     1081 cgccgagatg tcgatcagca cgccgtccgc gccggtggcc tcgatcctgg ccgcgaggtc
     1141 gtcctgcagc gccagggccg actggtcctg caggtcgacc tggatggtca ccagcaggtt
     1201 gcggcccatg cgcaggatgg ggatccgctc catggcgtgc tcaggccttc gccgccttgg
     1261 tcacgacata gccggcgcgc ttcatcgcga gttgcagcgc gtcggcaagc gtggccttgg
     1321 tcgtgatgcc ctgcaggtcg atgcccaggt ggacgatggt ctgcgcgatc tgcgggcgga
     1381 tgccgctgat gatgcagtcc gcgcccatga ggcggatcgc cgtcaccgtc ttgagcagat
     1441 gctgggcgac cagcgtgtcg acggtgggca cgccggtgat gtcgatgatc gcgagctccg
     1501 agcccgtctc gacgatgcgc tgcagcagcg actccatcac gagctgggtg cggcccgagt
     1561 ccagcgtgcc gatcatcggc actgccagca cgccctccca cagcttgacg accggcgtcg
     1621 acagctcgag cagttcctcc tgctggcgct ggatcacctc ctcgcgcgag cgctggtagg
     1681 tgttggtcgt ccattgcgcc atcttgtcca ccagcaggga agccgaccac atcgcgtcga
     1741 acagcccctg cggatcctgg ccccgcgatg ccttcagtcc ggtgaacagc gggcgcttga
     1801 gcgccatgac gaaatggctg gtctcggcag cgctggaacc ctgcgccgca cgcgaagcgg
     1861 acagcccgcc cagcaagttg cgcaggcggc cgaagccggg cgcgtcgttg aagcgttcga
     1921 ggtcggcatc gctgcgcagg gcctcgacca gcgcgatcac catgtcgcgc acgtcctgcg
     1981 tcgccgtcga gctcgcggcg ccggcagtgc ccgagctggc gatctccgac agccactcct
     2041 gctggaaccg ttcctggtcg gccgtgacgg cctccagcaa ggccttggtc tcgatattca
     2101 tgaggcaccc tcttcgcgaa ggcggcaagg gtaactcaac gtgacgcggc ggcagcaatt
     2161 gatcggtgtc agcgttcatt tcccgccgtt gtcctacaaa ccgggaaagg ccgtgccggc
     2221 gccggcggcg cccttgaacc gcgtcgacgc ggcgccagat tcggcattga atggtcgagt
     2281 cgatcattcg acgtgcatga atgcgagcgt tcactatccg tatttcgtcg tgagatagct
     2341 attcctgcaa tcaggtcaag gcgccgcgac acgccagcac tcttcgatcg agagtgctaa
     2401 tatccgcgga accgatcgcc cgaaaagcgg tcacacacag cacatgacca ccacccaagc
     2461 caccgcactg tccgtccgcg atccctgggc cctggtgccc ccgctcggga acctggacgc
     2521 ctacatctcg gcgatcaacc gcctgccgat gctgtcgcag gaggaggaga cgcgcctcgc
     2581 gaccaagctg cgcgacgagg gcgacctgca cgccgccagc gcgctggtga tgtcgcacct
     2641 gcgcctcgtc gtgtccgtct cgcgccagta cctcggctac ggcctgccgc atggcgacct
     2701 gatccaggaa ggcaacatcg gcctgatgaa ggccgtcaag cgcttcgatc ccgaccaggg
     2761 cgcgcgcctc gtcagctacg cgatgcactg gatcaaggcc gagatccacg agtacatcct
     2821 gaagaactgg cgcatggtca aggtcgcgac gaccaaggcc cagcgcaagc tgttcttcaa
     2881 cctgcgctcg ctcaagagca agctgaagga tgaggcgtcc gatgcggacg tccatcgcaa
     2941 cacgctcacc cgcgcccagg tcgagcgcat ggccagcgac ctgaacgtca agcccgagga
     3001 agtcctcgag atggagacgc gcatgtccgg cggcgacatc gcgatggagt cgcagaacga
     3061 cgacggcgag gagaccttcg cgccgatcgc ctacctggcg gacgaccgcg acgagccgac
     3121 ccgcgtgatc gagaaggccg cccacgaccg cctcgccggc cagggcatcg tcgaggcgct
     3181 ggatcagctc gatccgcgca gccgccgcat cgtcgaggag cgctacctca aggtgaacga
     3241 cgacgcctcg ggcgggatga cgctgcatga actcgccgcc gagtacggcg tgagcgccga
     3301 gcgcatccgc cagatcgagg tcgccgcgct gaagaagatg cgcaagacgc tcgagccgca
     3361 gcgcgcctga tcgacggcgt tgccccagga agaagaacgg acgccgcggc gtccgttttc
     3421 ttttcggccc gcaggccgcg gcggtcaggc cgcgtgcgac tgcgcgagca gcgcctggat
     3481 gtcgccggcg agctggccag ggtccatgtt ctggcgggcg aacaggcgga tgcggccctg
     3541 cggatcgaag atgtagacgc cggccgtatg gtccatcgtg taggtctccg gcgtcttgcc
     3601 gggcaccttc tcgtagaaga ccttgaactc cttggcgacc tcgcgcgtct gggcgtcgtc
     3661 gccgcgcagc gccaggaaat ccgggcggaa attggtcacg taggccttca gaagccccgg
     3721 cgtgtcgcgc gccgggtcga cggtgatgaa gacgacctgc agcctggaag catcggggcc
     3781 gagcgtcttg gcgacgccgg cgagctccgc catggtcgtg gggcaggcgt ccgggcattg
     3841 cgtgtagccg aagaagacgg ctgtgacctt gcccttgaag tcggccagcg tgcgcacctt
     3901 gccgtcgaag tcgggcaggc tgaactggcg cgcgtactcg gcgccggtga ggtcgatcgc
     3961 cgcgaacttg tggggcggcg ggggcggcgg cgcggccggg ccgcggtcgc agccggccag
     4021 gacggccagg ccgagcgcga gtggcagcgc ggcggcgagc aggagtcgtc gggtcgaggt
     4081 catggaggga gcattcaccg ggggatgtag tggtcgacca gcagcgccgc gaacagcagc
     4141 gacaggtaca ggatcgagaa gcggaaggtc ttgcgcgcga gggcgtcgct gtagtcgcgc
     4201 cacagccgga agccgtacca gcagaacacc agcccgagca ccagcgcggc cgcgaggtag
     4261 ggcaggccgc tcaggccgct gacgaagggc agcatcgtcc ccgccagcag cacgatggtg
     4321 tagaggaaca cgtgcagctt ggtgaagtcc ggcccgtggg tgacgggcag catcggcagg
     4381 ccggccttgc ggtagtcctc ggtacggtac agggcgagcg cccagaagtg cggcggggtc
     4441 cacaggaaga tgatcaggca cagcagccag gcgttcgcgt cggcgtggcc cgtcatcgcg
     4501 gcccagccga gcaccggcgg catcgcgccc gaggcgccgc cgatcacgat gttctgcggg
     4561 gtgcgcggct tgagcagcag cgtgtagacg atcgcatagc cgacgaaggt ggccagcgtc
     4621 agccacagcg tgagcgcgtt gacccaggcg agcagcagca ccgcgcccgc gccgcacagc
     4681 aggccggaga agacgagggt ctgcgcggtc gtcagctcgc cgcgcgccgt cgggcgccac
     4741 gcggtgcggg ccatgcgccg gtcgatctcc tgctcgacca ggcagttgaa cgcggccgca
     4801 gccgccgcga cgagccagat gcccgccgtc gcggccagcg ccggcaccag cggcggccag
     4861 ccgggcacgg ccaggaacat cccgatcacg gcacagaaca cgatgagctg gaccacgcgc
     4921 ggcttggtga gcgcgtagaa ctggcgccag cgcgacggcg gcgccggctg cttggcggac
     4981 gaagggatgg ggacggcggt gctcatgacg gggtgattct cgacttcagg agggcggcgg
     5041 cctcgcggcc caggcagatc accagggccg ccgcaccggc agtgtgcgcc agcgccgccg
     5101 ccatcggcca accaaggaca acattgctca ggccggtgcc ccattggacc accaggatcg
     5161 cgaggatcca gcccgcgcgc cgccgcgcct cgcgcccgcg ccgcgacaat cgcacgacca
     5221 gccacgccaa cgccgccgtc agcgcgagcg cgaacagccg gtgcatccac tggatcgcga
     5281 ccagcgcgct catggcaatc ggggcgccgt cggcgcgacg tccgagctcg cgggcgagcg
     5341 tgaagccctc ggcccagtcc atgcgcggcc accactggcc attgcaggtc gggaagccgc
     5401 tgcaggcgag caccgcgtag ttggtactga cccagccgcc cagcgccagt tgcaccagca
     5461 gcgccaccga ggcgattgcc agcgccgcgc ggtcgcgcgc gcgcacgagg ccgccgacgg
     5521 ccggcgcggg cgcctgcagg gccaggagcg cgagcaggcc catgccgccc agcaggtggc
     5581 ccgtgacgat ggccgggtag agcttccacg tcaccgtcag cgcgccgaac acgccctgcc
     5641 cgatgaccca ggccagcgtc accagggccc aggccagcgc gcgccggtcg cgggcgagcg
     5701 ccgcggccgc gaccaggccg atgatcagca cgcccagggc ggtcgcgaag tagcggtgga
     5761 tcatctcgat ccaggccttg cgctcggtga ccggccctcc gggcgctcgc gcctcggctt
     5821 cggcgatggg atgcgcggcc gcgaccgggc tggcggtcgc gtagcagccc ggccagtcgg
     5881 ggcagcccag gcccgagtcg ctcagccgcg tgtaggcgcc gaagcagacg aggtcgaagc
     5941 acaggaacag tgtcagcccg gcgagcgcgg ccacgcgctg tccgcggccg aggcgccgca
     6001 gcccgccgcg ccaccacgcg agcatcagca cgatggagac gatgcccagc gcgatcagcg
     6061 cgaggccggg cgcggcgtgc cagtcgtagg cgggcgtcgc gttcaccgcc cgtccttcgg
     6121 ccgatcccac cacgacgacg cgcgcagcag gcggtcgatg tcgcgcttga gcttgacggg
     6181 gtcggcgtcg accggtgcgc gcatcatcca gttgccgagc ggatcgacga tgtacaggtg
     6241 ctcctcgagc gcgtggtgcg gctcgggctc gagccagcgc gcgagcgcgt cgcggtcgac
     6301 gcgccgcacg atcaccgcgg gcggcgcctc gaccgccgac aggagcgtcg gcgcgaccgg
     6361 ccccgcgctg tcgacgagcc acaccttgtc gatgcggtcg cgctccgcgc ccaccatctc
     6421 gcgcagctgg cgttgcatga acagcagctt ctcgcagccc gcgccgcagc ccgccggcgc
     6481 gaccaccacg agcagccact ggcccttgag cgccgtcagc ggcgccgagc gcccgtccag
     6541 cgtctgcgcc ggcacccggt cgaggcccgg cagcgggcgc tgcggctcga tcagcgtcgc
     6601 atagttcgcg cgcccctgcg gccggatgac gtagtaggtg aagtaggaca gcaccaccgg
     6661 cagcgcgcac agcagcagga tcgcccacat cgtgcgccgg ccgcggccgc tgcgcgcgtc
     6721 ctgggcgtcg cgcacgggct cgggcgtgtc gtgcacggtg aagctcaagg actcgaggcc
     6781 gtcggccgcc aggcgcgcgg cggcgggcac gtcctcgtcg tccgcatgca cctcgtggga
     6841 cgaggcggcg agcggctcga gggggacggg ctgcgtcggg tcggtcggcg cgagggtcgg
     6901 cgcggtcggc gtcgggtcag gcatcggtcg gatcggaagc ggaagaggcg ccggcgcggc
     6961 ggcggaagaa ctgcgtccac gcccacaggc cgaggaaggc cacgctcatc gcgaaccact
     7021 gggcggcata gccgtagttc gtggcggtgc cgatgtcggg cgcgggccag tcgcgacgca
     7081 ggccgtcgga tggcgcgccg gggccctcgt cctcgaccac gatctccggc cgcaacgcga
     7141 gccccgcctc ggccgcgaag ggcccgggct cgagattctg ccgcaccggc ccgggcaccg
     7201 gcttgccgag ctcgatccag tgcgccggcc acggcgcgac atgtcccgcc acgcgtaccg
     7261 tcccgccggg cgcaggcacc ggcggcgccg ccatcggacg ggcatcgtcg cgcgcgatcc
     7321 agccgcgctg cacgaccacc gcgtcgccgg agggcagctt cagcggcgtc atcacgtaga
     7381 agcccgagcg cgcgtccatc gtccggttca tcaggaagac ggtgtgcgcg acgtcccagc
     7441 ggccctcgag ctcgatgcgg cgctgccact gcgcggcggc gccggccgcg tcggtcgcga
     7501 gcgctgcggc cggcaggggc ggttcgtggc tgcgctccgc caccagcgcg gcggcgctct
     7561 ccttctggtg cgcgcgggag agctgccaga cgcccagccg cgccgtcgtc gccgcgcaca
     7621 tcaccgcgac gacgagccag cccaggcgcg acgccgccgt cggcccacgg cgccgctcga
     7681 cggatgcgcc ctcggcattg cccgcgacgc catgcgggcc gggcgaaccc gcggaccgcg
     7741 ccggatcgcg ggtggaataa tcgtcggtca tgcgtatcgt gatcgtgggg ttcctggtgc
     7801 tcgtcctggc cgcgctcggc ggggccgggt tcttcatgct gcgccgcaag cccgacgacg
     7861 gccgcgccga caagcgcatg gcgcgcgcgc tggcggtgcg cgtcggactg tcggtgacgc
     7921 tgttcctgct gatcctcctg gcctacgcgc tcggctggat ccaaccgcgc ggcatcccgg
     7981 tcggcacctg agcgccgccg cagccggcgg ccagcaggtt cgaacgcctc gccgcgccgc
     8041 ggttccatcc cctcaaacga ggacgccgcc cgaggtcgag cggcgtcatt gtcgcgcgtc
     8101 gcgggcccca gcccgccgga cgctcaaagc cagtacacga ccacgtagag gcccagccag
     8161 acgacgtcga cgaagtgcca gtaccacgcc gcgccctcga agccgaagtg gcgcgtcggc
     8221 gtgaagtggc ccttctgcag ccgcatcgtg atgaagaaca gcatcagcat gccgacgaag
     8281 acgtggaagc cgtggaagcc tgtgagcatg aagaaggtcg agccgtagat gcccgacgac
     8341 agcttcaggt tcatcgccgt gtacgcctcg tggtactcgt aggcctggca gcacaggaag
     8401 ctcgcgccca gcagcacggt gatccacatc cacccgacgg ccttggcgcg gtggttggcg
     8461 atcagcgcgt ggtgcgcgat cgtcagcgtc acgccggagg acagaaggat cgcggtgttg
     8521 agggtcggga tcggccacgg gcccatggta tggaaggcgg cgatcgtgtc ggccggcgag
     8581 gcggtcgcgc cggcctgcag gctcggccag acggcgtgga agtcgggcca cagcagctgg
     8641 tggtcgaggt tgcccagcat cggcagcgcg atgttgcggg cccagaacag cgcgccgaag
     8701 aacgacgcga agaacatcac ctcggagaag atgaaccagc tcatgctcca gcggaacgag
     8761 gtgtccacgc ggtcggagta ctggccgccc tcgctctcgc cgatggcctg cgagaaccac
     8821 tgcgcgagcg tcgacagcca gacgacgagc ccggccagca acacccactt gccccagccg
     8881 ttgtcgttga tccattgcgc gccgccgaag atcaccagca gcatgcccgc ggcgagcgtg
     8941 accgggtagc gcgagggcgc cgggatgaag tagtgcgggg tgcttccgtg gggagtcgcg
     9001 gcggacatgg ctcggggtct cctggcttct cttcagaatc gattcgatgg ccggtcggtc
     9061 aatgcgacgc gcccgacagg ccgatgacga aatgcacgag cgcgatcagc gccagcacga
     9121 acagcgccgc ggccagcagc gccgcgatca ccacgtgcag cgggttgagc cgcttcacgt
     9181 cctgctcgtg atccttcgag cgccggatgc ccaggaagga ccaggcgacc gcgcggaagg
     9241 tctggccgaa cgcggccttg cgcgcgagcg gcgagtgcgg gtcgcgctcc ggctcgtcgg
     9301 caggcggcac gggttcgggc atgggcggct tcaggcatgc ggcgcgggcg gagcgtcgcc
     9361 ggacggagcc gccggcatgg tgccgccgac ctcgaagaac gagtaggaca aggtgatcgt
     9421 cttgatgtcc ttgggcagct tgtcgtcgac gaagaacgtg accggccacc gcttggactc
     9481 gcccggcgcg agcgtgtgct gcgtgaagca gaagcactgc accttgttga agtgcggctg
     9541 cgccccgccc ggggcgtagc tcgggatggc ctgcgccgtc atcgtgcgcg actgcgtgtt
     9601 cttgaactcg tacatcaccg tcgtcagcgc gcccggatgc acgacgaccg aggactgctc
     9661 gggatggaat tcccacgggc cgcggacgtt ggcgtcgaac tcgatcgtga cgctgcgcga
     9721 gaggtcgacc tgggaattgg ccgcgccggc gtcgacctgc ccatcgccat cgtcgcgcgc
     9781 gatcacgttg acacccagcg cctcgcagat cgaccggtac atcggcacca tcgcgtaccc
     9841 gaagccgacc atcagcacga cgaacaccgt caggccgacg agcatgcgcc ggttgtggcg
     9901 ccgcagcgcg gcaggacgtg ccgcggcgcg cgggttcgcg ggcgtggtgg aggtcgtcat
     9961 gggcgtcgca tcctcagagc ccgaacagga tcgccttggc gacgaacccg gcgaggaaga
    10021 cgaacgccac cgacgcgagc acgaggccga gccggaggtt cttgcggcgt tgctcgggcg
    10081 tcagggccat ggaggtctcg atgcggagac ggttcagccg atgaccttgg tggccgtctt
    10141 gtccagcttg ggcggcgtct cgaacgtgtg ccacggcgcg ggcgacggca cttcccactc
    10201 gaggccctcg gcgccttccc acgggcgttg cggcgcgggc tcgcccttgc cgcgcatcac
    10261 cggaatgacg acgaacagca ggaagtagac ctgcgacagg ccgaagccga ggccgccgat
    10321 cgaggccagc gcgttgaagt ccgcgaactg catcgggtag tccgcgatgc ggcgcggcat
    10381 gccggccagc ccgaggaagt gcatcgggaa gaacgtcacg ttgaacgtga tcagcgacgc
    10441 ccagaagtgg atcttgccgc gcgtctcgtt gtacatcacg cccgtccact tcggcgacca
    10501 gtagtagaag ccggagaaca tgccgaacag cgaaccggcg accagcacgt agtggaagtg
    10561 ggcgacgacg tagtaggtgt cctgcagctg gatgtcgatc ggcgccatcg agcagatcag
    10621 gcccgtgaag ccgcccatcg tgaacacgaa gatgaagccg accgcccaca gcatcggcgc
    10681 ctcgaaggtc atcgagccgc gccacatcgt cgcgagccag ttgaagacct tcacgcccgt
    10741 gggcaccgag atcagcatcg tcgcgtacat gaagaacagc tggcccgtga ccggcatgcc
    10801 cgtcgtgtac atgtggtgcg cccagacgat gaacgacagg atcgcgatcg acgccgtcgc
    10861 atacaccatg gaggtatagc cgaacaggcg cttgcgggcg aaggccggca ccacctggct
    10921 gatgatcccg aacgcgggca ggatcatgat gtagacctcg gggtggccga agaaccagaa
    10981 cacgtgctgg tacatcaccg ggtcgccgcc gccggccggg ttgaagaagc tcgtgtggaa
    11041 gtgacggtcc gtcagcgtca tcgtgatggc gccggccagc accggcatca cggcgatcag
    11101 caggtaggcg gtgatgagcc aggtccaggc gaacatcggc atcttcatca gcgtcatccc
    11161 gggggcgcgc atgttgagga tggtcacgat gatgttgatc gagcccatga tcgagctcgc
    11221 gcccatgatg tgcagcgcga agatcgagct gtccatcgac atgcccatct gcagcgtcag
    11281 cggcgcgtag agcgtccagc ccgccgcggg cgcgccgccg ggcatgaaga agccgcccgc
    11341 cagcatcagc gccgccggga tcagcagcca gaagctgagg ttgttcatgc gcgcgaacgc
    11401 catgtccgcg gccccgatct gcatcgggat catccagttc gcgaagccca cgaacgccgg
    11461 catgatggcc ccgaacacca tgatgagccc gtgcatcgtg gtgaactggt tgaacagctc
    11521 ggggttgaag tactgcaggc cggggcggaa cagctccagg cggatgcaca gcgccaggat
    11581 gccgccgatc aggaacatcg tgaacgagaa cagcaggtac agcgtcccga tgtccttgtg
    11641 gttggtcgcg aagagccagc gtcgccagcc ggtcgggtgg tggccgtgcg cgtggtcgtc
    11701 ctcgtggccg tggtcgatcg ggtggggcag aacagcactc atgacaggaa acctctcaac
    11761 gaacgcgggt gcgcggcgat cctggcggga ccgggcggcg gcttacttcg actcgtcggc
    11821 gagcacgtct gccggctgca cgacctggcc ggtgtggttg ctccacgaat tcttcgtgta
    11881 cgtgatcgcg gcggcgatgt cggtctccga cagcttgccc tgccatgccg gcatcgcctc
    11941 gacgccgttg accttgcggc cgtggagcac ggtgaggatc tgcgcgttct tgtcggcgtc
    12001 gttgacgacc ggcgagccgt cgagcgcctt gaccgcgccg ccgcccttgc cgttggcctg
    12061 gtggcagacg ccgcagttgg cgttgtagac cttctcgccg cgcgcgacga gcgggcccat
    12121 ctcccacacc ttcttcggat cgtcggccgc ggccgcgagc gccttgcgct gcgcggcgac
    12181 ccaggcggtg tagtccgcgg cgctcttgac gatcacgtgg atcggcatgt acgcgtgctc
    12241 cttgccgcag agctcggagc acatgccgta gtagtcgccg ggcttggtcg cgcggaacca
    12301 ggtgtcgcgc acgaagcccg ggatcgcgtc ctgcttcacg ccgaaggccg ggacgctcca
    12361 cgaatggatc acgtcgttgg cggtggtgat gacgcgcacc ttcctgtcga ccgggacgac
    12421 gagcgggttg tcgaccttga gcaggtagtc gttgccggcc gccttgtcct cttcggagag
    12481 ctcgcgctgc gcggtgtcga gcgtggagat gaacgacagg ccctcgccct cgcccttgac
    12541 gtagtcgtag ccccacttcc actggtagcc ggtgaccttg atggtcaggt cgggattggt
    12601 ggtgtccttc atcgcgacga ccgtgcgcgt cgcgaacgcg cccatcagga tgacgatgac
    12661 gaatggcacg accgtccagg cgatctcgac ggcgacgctc tcgtggaagt tggccgcctg
    12721 gtggccgacg ctcttgcggt gcttcacgat tgaatagaac atgaagccga acacgacgac
    12781 gaagatcgcc gtgcagacca ccagcatgcc gtagtggagg tccttgagct gttgcgcgat
    12841 gtcggtgacg gcgggctgga agtcgacgcc gttgaggcgg gggccgccgg gcaggtcggt
    12901 cacgccgtcg gcattcgggg cggcggcggc gaggccggcg gcggtcgacg cgagggcggc
    12961 cagtgcggcg cgcagcggca gttgcctggg gctgcaagtc gtcatcaatg tcgtcacgta
    13021 gttcgatcct tgttatcgac acgggcttcg cgcgcgcggc ccggtcggca tgatcagcgt
    13081 tcgcgcgctt tccctgtaca gggtttgcac agggtcgggg cgccttcaga cacaagactc
    13141 ggacgattct agggcaggtt ctccagccct accttgcggg tcgaccccga ctcagcatcg
    13201 atttcatgtc ctcgagcccg acttccgtgc gcgccgccac cttggcgcgc ggctgcggct
    13261 tgaacgcgtg gccgtagacg atctcgaact ccagcaccac gcgaccgtcg cggtcgcggc
    13321 gctcgccgag ggcctcgagc agcgcctcgc gccagcgcgg ggtgcgcagg cccgcggcgc
    13381 gcgcgcggtc ggtgttggcg cccagcgcgt gcagctcgtc gagcgccgcc tgcggcgagc
    13441 tccaggtcag gcgcagggtc tcctggtcca tcaccggatc ggcgaagccg gcctccacca
    13501 gcatgtcgcc gaggtcgtgc atgtccgtcc acggcgcata cggggtgccc catccgcggc
    13561 ggcggtaggc ctcggccagc tcgggcagcg tgcccgggcc cacggtcgtg aacatcagga
    13621 agccgtcgac cgcgagcgcg gcatgccagt cgcgcatcag cgtcggcgcg tccggcgccc
    13681 agtgcagcgc catgttcgac cacaccagct gcgcctgtcc cgcggggacg gccgactgcg
    13741 gcatggcgcg cggtcccgcc gcggccagtc ccagccaccg ccgccaggcg ggcgcggc
//
LOCUS       DASVLN010000148         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_383405, whole genome shotgun sequence.
ACCESSION   DASVLN010000148 DASVLN010000000
VERSION     DASVLN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383405"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VF453_03440"
     CDS             <1..1605
                     /locus_tag="VF453_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686731.1"
                     /translation="LLKLARAAGPEAILAGGALPAPVVRAIFWALALMALCAALVLTL
                     PLSVTRVVGSAALVALALACINAFGSFVLTFWPLRRGLPGLVSWALVLAVLASPFNDN
                     HAPLARSLAHRGPGPRPAIDVAYARWLAARGTADPVYVIATEGGGIRAAYWTAAVLEH
                     LAADPQRHFARDLFAISSVSGGSVGAGFWVAGERARRCEPSAEAVRPDVATRALTTDF
                     VSPAVGFMLFPDLMQRFVPHSFPLANRSRGLEESWQRAFAALPGRPMDRSLAELYDGC
                     PALPQLLVNATVAETGQRAVLTSLSTSSFVDVFGLPAGSAVDPSTDHQPLAGVLLQSA
                     RFPVVSPAGSVVDVHDPAHPLVTRLIDGGYFDNSGIVTALELIDGMRVVPDSPPIVLI
                     VISDATYDDCAREDGTTYCRSPTVPPERTAVRGPVAGTAWLNEPLPILTGLFDVRDSH
                     VLEAMARALRTTQVAVLDRSLAVSGETNAQRAAEVAAPLGWALSRSVTDMLDKQSAWV
                     AGRMPLQRPDWHPPTPLVPHAEDRHG"
BASE COUNT          173 a          639 c          590 g          245 t
ORIGIN      
        1 ctgctcaagc tcgcgcgggc cgcgggcccg gaggcgatcc tcgccggcgg cgccctgccc
       61 gccccggtcg tgcgtgcgat cttctgggcg ctcgcgctga tggccctgtg cgccgcactg
      121 gtgctcacgc tccccctgag cgtcacccgc gtggtcggca gcgcggccct ggtggcgctg
      181 gcgctcgcct gcatcaacgc cttcggcagc ttcgtgctga cgttctggcc actgcgccgc
      241 ggcttgccgg gattggtctc gtgggcgctc gtgctggcgg tgctcgccag tcccttcaac
      301 gacaaccatg cgccgctcgc gcgctcgctc gcgcatcgcg gcccggggcc gcgcccggcg
      361 atcgacgtcg cctacgcgcg ctggctcgcc gcgcggggca cggccgaccc ggtctacgtg
      421 atcgcgacgg agggcggcgg catccgcgcc gcgtattgga ccgccgcggt gctggagcac
      481 ctcgccgcgg atccgcagcg ccacttcgcg cgcgacctgt tcgcgatctc gtcggtctcg
      541 ggcggcagcg tcggcgcggg gttctgggtc gcgggcgaac gggcgcggcg ctgcgagccc
      601 tcggccgagg cggtgcgtcc ggacgtcgcg acgcgcgcgc tgacgaccga cttcgtctcg
      661 cccgccgtcg gcttcatgct ctttcccgac ctgatgcagc gcttcgtgcc gcattccttc
      721 ccgctggcca accgctcgcg cggcctcgag gagagctggc agcgcgcctt cgcggcgctg
      781 cccggacggc cgatggaccg cagcctggcc gagctgtacg acggctgccc cgcgctgccg
      841 cagctgctgg tcaacgccac ggtcgccgag acgggccagc gtgccgtgtt gacgagcctg
      901 tccacgagca gcttcgtcga cgtcttcggg cttcccgcgg gcagcgccgt cgacccgtcc
      961 accgaccacc agccgctcgc cggcgtgctg ctgcagagcg cgcgctttcc ggtcgtcagt
     1021 cccgcgggct ccgtcgtcga cgtgcacgat cccgcgcacc cgctcgtcac gcggctgatc
     1081 gacggcggct acttcgacaa ctcgggcatc gtgacggcgc tggagctgat cgacggcatg
     1141 cgcgtcgtcc cggattcgcc gcccatcgtg ctgatcgtga tctcggacgc gacctacgac
     1201 gactgcgcgc gcgaggacgg cacgacctac tgccgatcgc ccacggtgcc gcccgagcgc
     1261 acggcggtgc gcggcccggt cgcgggcacg gcctggctca acgagccgct gccgatcctc
     1321 acgggcctgt tcgacgtgcg cgactcgcac gtcctggagg cgatggcgcg ggcgctgcgg
     1381 acgacgcagg tcgccgtgct ggaccgctcg ctggcggtca gcggcgagac gaacgcgcag
     1441 cgcgccgccg aggtcgcggc gccgctggga tgggcgctct cccgcagcgt caccgacatg
     1501 ctcgacaagc agagcgcctg ggtcgccggc cgcatgccgc tgcagcggcc cgactggcat
     1561 cccccgacgc cgctcgtccc ccacgcggag gatcgccatg gctgagcgcg atgccccgcc
     1621 ggccgcgccg ctgcccgggt cgctggg
//
LOCUS       DASVLN010000149         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_383439, whole genome shotgun sequence.
ACCESSION   DASVLN010000149 DASVLN010000000
VERSION     DASVLN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383439"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /gene="aceE"
                     /locus_tag="VF453_03445"
     CDS             <1..1694
                     /gene="aceE"
                     /locus_tag="VF453_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703183.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX7686732.1"
                     /translation="DGAFVRKNFFGRDPKTLEMVAKMSDEDIWRLQRGGHDPQKVYAA
                     YHRAVNTVGQPTVLLVKTVKGFGMGKSGEAKNTAHQTKKLTDEDVRAFRDRFDIPIPD
                     EKLAELPFFKPEPHTPEMKYLHERRKALGGYLPHRRAKADEQLPVPPLSAFQAVLDPT
                     AEGREISTTQAYVRFLTALLRDKAIGPRVVPILVDEARTFGMEGLFRQIGIYNPLGQN
                     YTPVDKDQVMYYKEAKDGQILQEGINEPGGMSSWIAAATSYSTNNRIMVPFYVYYSMF
                     GFQRIGDLAWAAGDMQARGFLLGGTSGRTTLNGEGLQHEDGHSHILAGTIPNCVSYDP
                     TFAHEVAVIMQHGLVRMVERQENVYFYITLLNENYAMPGLKPGTEEQIVKGMYLLQEA
                     TQAGSLRVNLLGSGTILRESMAARELLAQDWGVGANVWSCPSFNELARDGQNAERWSL
                     LHPTEARRKSFVETQLEPHDGPVIASTDYIKNYAEQIRAFIPQGRSYKVLGTDGYGRS
                     DFRSKLRDHFEINRHYIVVAALKALADEGKLPAAKVAEAIAKYGIDADKSNPLYA"
     gene            1725..>1982
                     /locus_tag="VF453_03450"
     CDS             1725..>1982
                     /locus_tag="VF453_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7686733.1"
                     /translation="MALVEVKVPDIGDFKDVAIIELLVKPGDTIKAEQSLITVESDKA
                     SMEIPSSAAGVVKEMKVALGDKVSEGSTLLLLETAGGAAAPA"
BASE COUNT          379 a          690 c          652 g          261 t
ORIGIN      
        1 acgacggcgc cttcgtgcgc aagaacttct tcggccgcga cccgaagacg ctcgagatgg
       61 tcgccaagat gagcgacgag gacatctggc gcctgcagcg cggcggccac gacccgcaga
      121 aggtctatgc ggcctaccac cgcgcggtca acaccgtggg ccagccgacg gtgctgctgg
      181 tcaagacggt caagggcttc ggcatgggca agagcggcga ggcgaagaac accgcccacc
      241 agaccaagaa gctgaccgac gaggacgtgc gcgccttccg cgaccgcttc gacatcccga
      301 tccccgacga gaagctcgcc gagctgccgt tcttcaagcc cgagccgcac acgccggaga
      361 tgaagtacct gcacgaacgc cgcaaggcgc tcggcggcta cctgccgcac cgccgcgcca
      421 aggccgacga gcaactgccc gtgccgccgc tgtccgcgtt ccaggccgtg ctcgacccga
      481 cggccgaggg ccgcgagatc tcgacgacgc aggcctacgt gcgcttcctg accgcgctgc
      541 tgcgcgacaa ggcgatcggc ccgcgcgtgg tgcccatcct ggtcgacgag gcgcgcacct
      601 tcggcatgga aggcctgttc cgccagatcg gcatctacaa cccgctgggc cagaactaca
      661 cgccggtcga caaggaccag gtgatgtact acaaggaagc caaggacggc cagatcctgc
      721 aggagggcat caacgagccc ggcggcatgt cgagctggat cgcggcggcg acgtcgtact
      781 cgacgaacaa ccgcatcatg gtgccgttct acgtgtacta ctcgatgttc ggcttccagc
      841 gcatcggcga cctcgcctgg gccgccggcg acatgcaggc gcgcggcttc ctgctgggcg
      901 gcacctccgg ccgcacgacc ctcaacggcg agggcctgca gcacgaggac ggccacagcc
      961 acatcctggc cggcacgatc cccaactgcg tcagctacga ccccaccttc gcccacgagg
     1021 tcgcggtgat catgcagcac ggcctcgtgc gcatggtgga gcgccaggag aacgtctact
     1081 tctacatcac gctgctgaac gagaactacg cgatgccggg cctgaagccg ggcaccgagg
     1141 agcagatcgt caagggcatg tacctgctgc aggaggccac gcaggccggc tcgctgcggg
     1201 tgaacctgct cggctcgggc acgatcctgc gcgagtcgat ggcggcgcgc gagctgctcg
     1261 cgcaggactg gggcgtcggc gccaacgtct ggagctgccc gagcttcaac gagctcgcgc
     1321 gcgacggaca gaacgccgag cgctggagcc tgctgcaccc gaccgaggcg cggcgcaagt
     1381 ccttcgtcga gacgcagctg gagccccacg acggcccggt catcgcgtcc accgactaca
     1441 tcaagaacta cgccgagcag atccgcgcgt tcattccgca gggccgctcg tacaaggtgc
     1501 tgggcaccga cggctacggc cgcagcgact tccgcagcaa gctgcgcgac cacttcgaga
     1561 tcaaccgcca ctacatcgtg gtcgccgcgc tcaaggcgct ggccgacgag ggcaagctgc
     1621 cggcggccaa ggtcgccgag gcgatcgcca agtacggcat cgacgccgac aagtccaatc
     1681 cgctctacgc ctgacccggc accccacaac acggagacga acccatggca ttggtggaag
     1741 tgaaggtgcc cgacatcggc gacttcaagg acgtcgcgat catcgagctg ctggtcaagc
     1801 cgggcgacac gatcaaggcg gagcagagcc tgatcacggt cgagagcgac aaggcgtcga
     1861 tggagatccc gtcctcggcg gccggcgtgg tcaaggagat gaaggtcgcg ctcggtgaca
     1921 aggtcagcga gggctcgacc ctgctgctgc tcgagaccgc gggcggtgcg gccgctcccg
     1981 ct
//
LOCUS       DASVLN010000150         3140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_8267, whole genome shotgun sequence.
ACCESSION   DASVLN010000150 DASVLN010000000
VERSION     DASVLN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8267"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..3029
                     /gene="uvrA"
                     /locus_tag="VF453_03455"
     CDS             42..3029
                     /gene="uvrA"
                     /locus_tag="VF453_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7686734.1"
                     /translation="MRAAESQAIRVRGARTHNLKNIDLDIPKHALVVITGLSGSGKSS
                     LAFDTLYAEGQRRYVESLSAYARQFLQLMDKPDVDVIEGLAPAISIEQKATSHNPRST
                     VGTVTEIHDYLRLLFARAGTPYCPDHDLPLAATPVAQMVDAVLALPQETRLLVLAPVV
                     RDRKGEFVELFADMQAQGYVRFRVDGQIVEASDLPKLKKTEKHDIDIVIDRLRTRPDA
                     RQRLAESFEAALRIADGRALALEMDSGAEHLFSSRFACPVCSYSLPELEPRLFSFNSP
                     VGACPGCDGLGQVTAFDPERVVAFPSLSLAAGAVKGWDRRNGYTFSMIEAVAAHLGFD
                     VDAPWESLPERAREVLLNGSGDEEIEFTYAAETGAKSRRAAVKRKHPFEGILPNFERR
                     FRETDSAAVREDLARYQAARACPACHGTRLRREARHVFLVNADPLPAVGRGQPPMAAG
                     GLAEERPGAAACGIAPSAQRKPIYEIEHATLSDCLAYFEQLRLEGARADIADKVVREI
                     RSRLVFLNNVGLNYLSLDRSADTLSGGEAQRIRLASQIGSGLTGVMYVLDEPSIGLHQ
                     RDNDRLIATLRHLRDLGNSVLVVEHDEDMILASEYCIDMGPGAGVHGGRVIAHGTPAE
                     VAANPESLTGRYLARTLRIEVPGERHPWRREPADGDPKNAPALRVRGARGNNLKDVTV
                     DIPVGLLTCVTGVSGSGKSTLVNDTLYAAVARELYGSHDEPAAHEAIEGLEAFDKVIN
                     VDQSPIGRTPRSNPATYTGLFTPIRELFAEVPAARERGYGPGRFSFNVSGGRCESCQG
                     DGVLKVEMHFLPDVYVPCDICHGKRYNRETLEVLYKGKHIAQVLELTVEDALAFFSAV
                     PNIARKLQTLMDVGLGYITLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILDEPTT
                     GLHFADIQLLLRVLHQLRDAGNTIVVIEHNLDVIKTADWLIDMGPEGGAGGGTVVVEG
                     TPETVAACPRSHTGAYLKRLLAS"
BASE COUNT          470 a         1182 c         1073 g          415 t
ORIGIN      
        1 ccgtcggggc cgagccgtcg gccgcctacc tcgcccgcgc gatgcgcgcg gccgagtcgc
       61 aagccatccg cgtgcgcggc gcccgcacgc acaacctgaa gaacatcgac ctcgacatcc
      121 ccaagcacgc gctggtggtg atcacggggc tgtcgggctc gggcaagtcc agcctcgcct
      181 tcgacacgct gtacgcggag ggccagcgcc gctacgtcga gagcctgtcg gcctatgcgc
      241 gccagttcct gcagctgatg gacaagcccg acgtcgacgt gatcgagggc ctcgcgcccg
      301 cgatctcgat cgagcagaag gccaccagcc acaacccgcg ctcgacggtg ggcaccgtca
      361 ccgagatcca cgactacctg cgcctgctgt tcgcccgcgc cggcacgccg tactgccccg
      421 accacgacct gccgctcgcc gccacgcccg tcgcgcagat ggtggacgcg gtgctcgcgc
      481 tgccccagga gacgcgcctg ctcgtgctcg cgccggtcgt gcgcgaccgc aagggcgagt
      541 tcgtcgagct gttcgccgac atgcaggcgc aaggctacgt gcgctttcgc gtcgacggcc
      601 agatcgtcga ggcctccgac ctgcccaagc tgaagaagac cgagaagcac gacatcgaca
      661 tcgtgatcga ccgcctgcgc acgcgccccg acgcgcgcca gcgcctggcc gagagcttcg
      721 aggccgcgct gcgcatcgcc gacgggcgcg ccctggcgct cgagatggac tcgggcgccg
      781 agcacctgtt ctcctcgcgc ttcgcctgcc cggtgtgcag ctactcgctg cccgagctcg
      841 agccgcggct gttctcgttc aactcgccgg tcggcgcctg cccgggctgc gacggcctgg
      901 gccaggtcac ggccttcgac ccggagcgcg tcgtcgcctt cccgtcgctg agcctggccg
      961 cgggcgcggt caagggctgg gaccggcgca acggctacac cttctcgatg atcgaggcgg
     1021 tcgccgccca tctcggcttc gacgtcgacg caccgtggga atcgctgccc gaacgcgcgc
     1081 gcgaggtgct gctgaacggc tcgggcgacg aggagatcga gttcacctac gccgcggaga
     1141 cgggcgcgaa gtcgcgccgg gccgcggtca agcgcaagca tccgttcgaa ggcatcctgc
     1201 ccaacttcga gcgccgtttc cgcgagaccg actcggccgc ggtgcgcgag gatctcgccc
     1261 gctaccaggc cgcgcgcgcc tgcccagcct gccacggcac ccgcctgcgc cgggaagctc
     1321 gccacgtgtt cctggtgaac gcagaccccc tgcccgcggt cggcaggggc cagcccccca
     1381 tggcggcggg cggccttgcc gaggagcggc cgggcgcagc cgcctgcggc atcgccccgt
     1441 cggcccagcg caagcccatc tacgagatcg agcatgccac gctgtccgac tgcctcgcct
     1501 acttcgagca gctgaggctc gagggcgcgc gcgccgacat cgccgacaag gtggtgcgcg
     1561 agatccgcag ccggctcgtg ttcctcaaca acgtcggcct gaactacctg agcctggatc
     1621 gcagcgccga cacactctcg ggcggcgagg cgcagcgcat ccggctcgcg tcgcagatcg
     1681 gctccgggct gacgggcgtg atgtacgtgc tcgacgagcc gtccatcggc ctgcaccagc
     1741 gtgacaacga ccgcctgatc gcgacgctgc gccacctgcg cgacctcggc aactcggtgc
     1801 tggtggtcga gcacgacgag gacatgatcc tcgcctccga gtactgcatc gacatgggcc
     1861 cgggcgccgg cgtgcacggc ggccgcgtga tcgcgcacgg cacgccggcc gaggtggcgg
     1921 ccaatccgga gtcgctcacc ggccgctacc tcgcacgcac gctgcgcatc gaggtgcccg
     1981 gcgagcgcca cccgtggcgc cgcgagcccg cggacggcga cccgaagaac gcgccggcgc
     2041 tgcgcgtgcg cggcgcgcgc ggcaacaacc tgaaggacgt cacggtcgac atccccgtgg
     2101 ggctgctgac ctgcgtgacg ggcgtctccg gctcgggcaa gtcgacgctg gtcaacgaca
     2161 cgctgtacgc cgccgtcgcg cgcgagctgt acggcagcca cgacgagccc gccgcgcacg
     2221 aggcgatcga gggcctggag gccttcgaca aggtcatcaa cgtcgaccag agcccgatcg
     2281 gccgcacgcc gcgctccaac ccggcgacct ataccgggct gttcacgccg atccgcgagc
     2341 tcttcgccga ggtgcccgcg gcgcgcgagc gcggctacgg cccgggccgc ttcagcttca
     2401 acgtctcggg cggccgctgc gagagctgcc agggcgacgg cgtgctcaag gtggagatgc
     2461 actttctccc cgacgtctac gtgccctgcg acatctgcca cggcaagcgc tacaaccgtg
     2521 agacgctgga ggtcctctac aagggcaagc acatcgccca agtgctggag ctgaccgtcg
     2581 aggacgcgct ggcgttcttc tccgccgtgc ccaacatcgc gcgcaagctg cagacgctga
     2641 tggacgtcgg cctgggctac atcaccctgg ggcagagcgc gacgacgctg tccggcggcg
     2701 aggcgcaacg cgtgaagctg gcgctggagc tgtccaagcg cgacaccggc cgcacgctgt
     2761 acatcctcga cgagccgacc accggcctgc acttcgcgga catccagctg ctgctgcgcg
     2821 tgctgcacca gctgcgcgac gcgggcaaca ccatcgtcgt catcgagcac aacctggacg
     2881 tcatcaagac ggccgactgg ctgatcgaca tgggcccgga gggcggcgcc ggcggcggga
     2941 cggtggtggt cgaaggcacg ccggagaccg tcgcggcatg cccgcgcagc cacacgggcg
     3001 cctacctcaa gcggctgctg gcgagctgag ggccgcggcc gcgccctctc agtcggcgcg
     3061 caccgcgcgg tcgcgcccgg cagccttggc gcgggccagc gcgccctgtg cacgggccag
     3121 gaagtcggcc gccgcctctc
//
LOCUS       DASVLN010000151         7276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_61122, whole genome shotgun sequence.
ACCESSION   DASVLN010000151 DASVLN010000000
VERSION     DASVLN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61122"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VF453_03460"
     CDS             complement(<1..278)
                     /locus_tag="VF453_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686735.1"
                     /translation="MAARPPPTLRHLLAHRLAWRLVAIFVGAAMVPLALSDWLATTVL
                     GGVATRLDHERRALAVHDVSLQLLDRLTLARAMLRRIADSTPGPAAGAA"
     gene            complement(278..742)
                     /locus_tag="VF453_03465"
     CDS             complement(278..742)
                     /locus_tag="VF453_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7686736.1"
                     /translation="MKIPASPRPAAGYTLVELSVIVLLVGILTAIALPFYNSYRDRIR
                     VSHAIMSIQAMEASIDFYRTQNGALPGSLADMQLQGSLDPWGNPYVYYNIQVNGKGHA
                     RKDHALNPLNTDYDLYSAGADGQTKSQITQKDSVDDVIRASNGAFVGLASDF"
     gene            complement(846..1208)
                     /gene="fliE"
                     /locus_tag="VF453_03470"
     CDS             complement(846..1208)
                     /gene="fliE"
                     /locus_tag="VF453_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX7686737.1"
                     /translation="MDVRLKPFDFNAAVARAGLRPDGTPLAGGPSSIGADLGAKGVSG
                     PAKAGFGSALSDALKGVSDLQNQASWMQDQLQMDNPTVSLEQTMVTMQKAQIGFQATL
                     SVRNRLVQAYTDIMNMQV"
     gene            1474..3147
                     /gene="fliF"
                     /locus_tag="VF453_03475"
     CDS             1474..3147
                     /gene="fliF"
                     /locus_tag="VF453_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX7686738.1"
                     /translation="MEQALAPAAVPLPDAAPGPLARLAAMPAAAKMKVGGGLAALVAV
                     VIAIGLWSSQGDYRVLFANLPDKEGGAVVAQLAQMQVPYKFAEGGSAILVPADKVNEV
                     RLKLASQGLPKSSVVGFELMDNARFGQTQTQERVNLQRALEGELTRTISSIDAVESAR
                     VHLALPNQNGFFREQQKPSASVVLMLRGGRVLDRAQLAGIVHLVASSVPELQPKDVSV
                     LDQTGTLLTQNGDHDGQGLDPTQLQYVNQMEGGYVKRIQELLEPVVGAANLRATVTAD
                     VDFSQSDATSEQFKPNQGDPKDATVRSQQLSDTGTGANAVPSGVPGATSNTPPVPATA
                     PINGASAPLQAAQGGSVGPNGHRETVTNYEVDRTVRVARGATGLVRRLNAAVVVNQKT
                     TTDAKGKTTTTPLTQEEMDKLTALVQEAIGYDKDRGDSVKVINAPFKVEAAPKEEELP
                     LWKQQWLLDLLRAGGVPAGLTLVALAVLFGLVRPAVMAALAKPVEVEKAELDVVAGDH
                     EEVGMDALPEILEAPQMARKLESARQLAKENPIAVANIVRDWVNGEASA"
     gene            3178..4191
                     /gene="fliG"
                     /locus_tag="VF453_03480"
     CDS             3178..4191
                     /gene="fliG"
                     /locus_tag="VF453_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX7686739.1"
                     /translation="MPVSSKSDDQGVEDAAILLMSLGEEEAANVFKHLAPKEVQKLGE
                     TIARMKSITRERMDGVLDKFGTIAQEQHVLVQDTDEYVKSVLRKALGEDKANLLIDRI
                     LQGSDVTGIESLKWMDAGSVAELLRNEHPQIVAAILVHLDYDQSSGVLKSFTERQRNE
                     VLIRIATLDGIQPSALKDLNEVMSKVLAGGEKLKKASLGGVKTAAEIINLMGSSVETS
                     ALDYIREADNELAQKIMDNMFTFDDLNKLDDKGFQQLLKEVQSESLVIALKGATPELR
                     EKVFRNMSSRAAETLREDLESRGPVRVSEVESEQKEMLKIVRRLVDEGQIVLASGGAD
                     DFL"
     gene            4178..4972
                     /locus_tag="VF453_03485"
     CDS             4178..4972
                     /locus_tag="VF453_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEX7686740.1"
                     /translation="MTSSKSTLHARMAEREADRMAPVPPRGAPQARFIPREELEAFAA
                     WRPGSFGGGGAPSPHPSERPENKVHAAPPPPPPPPTEEEWLEEVRHAREAGYQDGYRD
                     GQVALEAFKEQFAKQMAAQFGQMLQTLDAQWNGLEEKMALAVTRTAVQLARQVVRHEL
                     DTRPEIVAKVAQEAVGAIVLSARHLCVRVHPADHAHVAEGAGEALKARDARLVADPAI
                     EPGGCVVDSDLGHVDARIATRWAQAAAVFGSQDGWDGDAAADGEAR"
     gene            4981..6381
                     /gene="fliI"
                     /locus_tag="VF453_03490"
     CDS             4981..6381
                     /gene="fliI"
                     /locus_tag="VF453_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEX7686741.1"
                     /translation="MPERAARWDRYLADIENYATVPQPLEMQGTLVRVTGLVLEAAGV
                     RVPVGAVCDVSGAGGEQPVSAEVVGFDGDRAFLMPTGELHGLSSGAKVVPRAVPHLPP
                     RFGEENHPWRRSEDRGLHLPMGAGLLGRVVDAHGQPLDRLGPLRDVRDEPMVRRSINA
                     MERDPVRQPLDTGVRAINSMLTVGRGQRIGLFAGTGVGKSVLLGMMARYTQADVIVVG
                     LIGERGREVKEFIEDILGEEGLARSVVVAAPADAPPLVRMQGASYATAVAEHFRDRGQ
                     HVLLLMDSLTRYAMAQREIALAIGEPPATRGYPPSCFAKLPQLVERSGNGAPGGGSIT
                     AFYTVLTEGDDPQDPIADAARGILDGHIVLSRELAESGHFPAIDVEKSISRVMTSVAP
                     RDHIAAARVARQALSRMTKARDLIQLGAYQPGADPELDAAVKMAPKLTALLQQDMHER
                     ASMAESKHTLNKAITL"
     gene            6398..6853
                     /gene="fliJ"
                     /locus_tag="VF453_03495"
     CDS             6398..6853
                     /gene="fliJ"
                     /locus_tag="VF453_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX7686742.1"
                     /translation="MKRLQMLFTLLDREQKRRDDARAVVQGALRNVEAQQQQADGLAS
                     YRVEYCAKWSAKFQTAATIEILRSYHGFLARLDQAVAQQQQVVAHAQRQAELARQKLV
                     EREIRVATVERLIQRRQALLAKIADRRDQKNLDEMAARRALPRELAGMA"
     gene            6910..>7276
                     /locus_tag="VF453_03500"
     CDS             6910..>7276
                     /locus_tag="VF453_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686743.1"
                     /translation="MAVNAAHSHAHPSKASHSAANAIAAPRAGAHVRARADADADAGG
                     EGFAAQLMRAQPQEHDAAPKSDSKDASAKDKDDKSAKDDRKDAAADGTQKQPDPSTLP
                     WPPQAQTTAALAAQSAPAES"
BASE COUNT         1160 a         2587 c         2609 g          920 t
ORIGIN      
        1 gccgcgcccg ccgcggggcc gggcgtggag tccgcgatcc ggcgcagcat cgcgcgtgcg
       61 agcgtgaggc ggtcgagcag ctgcagcgag acgtcgtgca ccgccagcgc gcgccgctcg
      121 tggtcgagcc gcgtcgccac gccgccaagc acggtggtgg cgagccagtc cgacagtgcc
      181 agcggcacca tggccgcacc gacgaagatc gccaccagcc gccaggcgag ccggtgcgcc
      241 agcaggtggc ggagcgtcgg cggcggccgc gcggccatca gaagtccgag gccaggccga
      301 cgaaggcccc gttgctcgcg cggatcacgt cgtcgacgct gtccttctgc gtgatctggc
      361 tcttggtctg gccgtcggcg ccggcgctgt agaggtcgta gtcggtgttg agcgggttga
      421 gcgcgtggtc cttgcgcgcg tggccctttc cgttgacctg gatgttgtag tagacgtacg
      481 ggttgcccca ggggtcgagc gaaccctgga gctgcatgtc cgcgagcgag cccggcagcg
      541 cgccattctg cgtacggtag aagtcgatgc tcgcctccat ggcctggatg gacatgatgg
      601 catgcgagac gcggatgcgg tcgcggtatg agttgtagaa aggcagcgcg atggcggtca
      661 ggatgccgac cagcaggacg atgacgctca actcgaccag tgtgtagccg gcggccgggc
      721 gtggcgaagc ggggatcttc atcgggggat catcggctga cgccggcccg cgcacagcgc
      781 gcggggggga atgccgcgtg caatattgac ggatgacagc gccgtcctgc gcccggatgg
      841 ccaggtcaaa cctgcatgtt catgatgtcg gtgtaggcct gcaccaggcg gttgcgcacc
      901 gacagggtgg cctggaagcc gatctgggcc ttctgcatcg tgaccatcgt ctgctcgagg
      961 ctgacggtgg ggttgtccat ctgcaactgg tcctgcatcc agctggcctg gttctgcagg
     1021 tcgctgacgc ccttgagcgc gtccgacagt gcgctgccga acccggcctt ggccgggccc
     1081 gagacgccct tggcgccgag gtcggcgccg atggacgacg ggccgcccgc caggggcgtg
     1141 ccgtccggcc gcaggcccgc gcgggcgacg gcggcgttga agtcgaaagg cttgagtctg
     1201 acgtccatca cggctgctcc ccgctcgccg gtccggacgc ggccgggcgg ccacggaagg
     1261 cggcgctcgg gcggacgggg gattcggctg gcatggtcgg aactttagtg gcgcggcacg
     1321 cggacgaagg cgggatctgg cggccgaacg gcggccttct cgcgccttct gcccgggggc
     1381 gattcctcaa gaatcctccc aatgcgccga ggccgggtct cgcctgcccg gcgcgccact
     1441 cgacaaatcc cgcgaaccgc cgtagaccac ttcatggaac aagccctcgc tcctgccgcc
     1501 gttcccctgc ccgacgccgc gcccgggccg ctcgcccgcc tggcggcgat gccggccgcg
     1561 gcgaagatga aggtcggcgg cggcctggcc gcgctggtgg cggtggtcat cgcgatcggc
     1621 ctgtggagct cccagggcga ctaccgggtg ctgttcgcca acctgcccga caaggaaggc
     1681 ggcgcggtcg tcgcccagct cgcgcagatg caggtgccct acaagttcgc cgagggcggc
     1741 tcggccatcc tggtgccggc cgacaaggtc aacgaggtgc gcctgaagct ggcctcgcag
     1801 ggactgccca agtcgagcgt cgtcggcttc gagctgatgg acaacgcccg cttcggccag
     1861 acccagacgc aggagcgcgt caacctgcag cgcgcgctcg agggcgagct cacgcgcacg
     1921 atcagctcga tcgacgccgt cgagagcgcc cgcgtgcacc tggcgctgcc caaccagaac
     1981 ggcttcttcc gcgagcagca gaagcccagc gcctcggtcg tgctgatgct gcgcggcggc
     2041 cgcgtgctcg atcgcgcgca gctggccggc atcgtgcacc tggtggcctc cagcgtgccc
     2101 gagctgcagc ccaaggacgt cagcgtcctc gaccagaccg gcacgctgct gacccagaac
     2161 ggcgaccacg acggccaggg cctcgacccg acgcagctgc agtacgtcaa ccagatggaa
     2221 ggcggctacg tcaagcgcat ccaggagctg ctggagccgg tcgtcggcgc cgccaacctg
     2281 cgcgcgaccg tcaccgcgga cgtcgacttc tcgcagagcg acgcgacctc cgagcagttc
     2341 aagccgaacc agggcgaccc gaaggacgcg acggtgcgca gccagcagct gtcggacacc
     2401 ggcaccggcg ccaacgcggt gccctcgggc gtgccgggcg cgacgagcaa caccccgccg
     2461 gtacccgcca ccgcgccgat caacggcgcc tcggccccgc tgcaggcggc gcagggcggc
     2521 agcgtcggcc cgaacggcca ccgcgagacc gtcaccaact acgaggtcga ccgcaccgtg
     2581 cgcgtggcgc gcggcgccac cggcctggtg cgccgcctga acgcggcggt cgtcgtcaac
     2641 cagaagacca ccaccgacgc caagggcaag accaccacga cccccctcac gcaggaggag
     2701 atggacaagc tcaccgccct cgtgcaggag gcgatcggct acgacaagga tcgcggcgat
     2761 tcggtcaagg tgatcaacgc gccgttcaag gtcgaggccg cgccgaagga agaggagctg
     2821 ccgctctgga agcagcagtg gctgctcgac ctgctgcgcg ccggcggcgt gccggccggc
     2881 ctgacgctcg tggcgctggc cgtgctgttc gggctcgtcc gccccgcggt catggcggcg
     2941 ctggccaagc cggtcgaggt cgagaaggcg gagctcgacg tcgtcgccgg cgaccacgag
     3001 gaggtcggca tggacgcgct gcccgagatc ctcgaggccc cgcagatggc caggaagctg
     3061 gagagcgcac gccagctggc caaggaaaac ccgatcgcgg tcgccaacat cgtgcgcgac
     3121 tgggtcaacg gcgaagcgtc ggcctgagcg ccggacgaca gcgaacgcga gagcaccatg
     3181 cctgtgtctt cgaaatcgga cgatcagggc gtcgaggacg ccgcgatcct gctcatgtcg
     3241 ctgggcgagg aggaggcggc caacgtcttc aagcacctcg cgcccaagga agtgcagaag
     3301 ctgggcgaga ccatcgcccg catgaagtcg atcacgcgcg agcgcatgga cggcgtgctc
     3361 gacaagttcg gcaccatcgc ccaggagcag cacgtgctgg tgcaggacac cgacgagtac
     3421 gtgaagtccg tgctgcgcaa ggcgctcggc gaggacaagg ccaacctgct gatcgaccgc
     3481 atcctgcagg gcagcgacgt caccggcatc gagtcgctga agtggatgga cgcgggctcg
     3541 gtggccgagc tgctgcgcaa cgagcacccg cagatcgtcg ccgcgatcct ggtgcacctg
     3601 gactacgacc agtcctcggg cgtgctgaag tcgttcaccg agcgccagcg caacgaggtg
     3661 ctgatccgca tcgcgacgct cgacggcatc cagccctcgg cgctgaagga cctcaacgag
     3721 gtgatgagca aggtgctcgc gggcggcgag aagctgaaga aggcgtcgct gggcggcgtc
     3781 aagacggccg ccgagatcat caacctgatg ggctcgtccg tggagacctc ggcgctcgac
     3841 tacatccgcg aggcggacaa cgagctggcc cagaagatca tggacaacat gttcacgttc
     3901 gacgacctga acaagctcga cgacaagggc ttccagcagc tgctcaagga agtgcagagc
     3961 gagtcgctcg tcatcgcgct caagggcgcg acgcccgagc tgcgcgagaa ggtgttccgc
     4021 aacatgtcca gccgcgccgc cgagacgctg cgcgaggatc tcgagtcgcg cggcccggtg
     4081 cgcgtctccg aggtcgagtc ggagcagaag gagatgctga agatcgtccg ccgcctggtc
     4141 gacgaaggcc agatcgtgct cgcgagcgga ggcgcggatg acttcctcta agtcgaccct
     4201 gcacgcccgc atggccgagc gcgaggccga ccgcatggcc ccggtgccgc cgcgcggcgc
     4261 cccgcaggcg cgcttcatcc cgcgcgagga gctcgaggcc ttcgccgcct ggcgccccgg
     4321 ctcgttcggc ggcggcggcg cgccctcgcc gcatccgtcc gagcgtcccg agaacaaggt
     4381 gcatgccgcg ccgccgcccc cgccgccgcc gccgaccgag gaagagtggc tcgaggaagt
     4441 gcgccacgcg cgcgaggccg ggtaccagga cgggtatcgc gacggccagg tcgcgctcga
     4501 ggccttcaag gaacagttcg ccaagcagat ggccgcgcag ttcggccaga tgctgcagac
     4561 gctcgatgcc cagtggaacg gcctcgagga gaagatggcc ctggccgtga cgcgcaccgc
     4621 ggtgcagctg gcgcgccagg tcgtgcgcca cgagctcgac acgcggccgg agatcgtcgc
     4681 caaggtcgcc caggaagccg tcggcgccat cgtgctgtcg gcgcgccacc tgtgcgtgcg
     4741 cgtccatccg gccgaccacg cccacgtcgc cgagggcgcc ggcgaggcgc tgaaggcgcg
     4801 cgacgcgcgg ctcgtcgccg atccggccat cgagccgggc ggctgcgtgg tcgactccga
     4861 cctcggccac gtcgacgccc gcatcgccac gcgctgggcg caggccgcgg ccgtgttcgg
     4921 cagccaggac ggctgggacg gcgatgccgc cgccgacggg gaggcgcgct gatgctggcg
     4981 atgcccgaac gcgcggcgcg gtgggaccgc tatctggccg acatcgagaa ctacgccacc
     5041 gtcccgcagc cgctggagat gcagggcacg ctggtgcgcg tcacgggcct ggtgctcgag
     5101 gccgccggcg tgcgcgtgcc cgtgggtgcc gtgtgcgacg tcagcggcgc cggcggcgag
     5161 cagccggtct cggccgaggt cgtcggcttc gacggcgatc gcgccttcct catgcccacg
     5221 ggcgagctgc acggcctgtc cagcggcgcg aaggtggtgc cgcgcgcggt gccgcacctg
     5281 ccgccgcgct tcggcgagga gaaccacccc tggcgccgca gcgaggatcg cggcctgcac
     5341 ctgccgatgg gcgcgggact gctgggccgc gtcgtcgacg cgcacggcca gccgctcgac
     5401 cgcctcggcc cgctgcgcga cgtgcgcgac gagccgatgg tgcgccgctc tatcaacgcg
     5461 atggagcgcg acccggtgcg ccagccgctg gacaccggcg tgcgcgccat caactcgatg
     5521 ctgacggtgg gccgaggcca gcgcatcggc ctgttcgccg gcaccggcgt cggcaagtcg
     5581 gtgctgctgg gcatgatggc gcgctacacg caggccgacg tgatcgtcgt cgggctgatc
     5641 ggcgagcgcg gccgcgaagt caaggaattc atcgaggaca tcctcggcga ggaagggctg
     5701 gcgcgctcgg tcgtcgtcgc ggcgccggcc gatgcgccgc cgctcgtgcg catgcagggc
     5761 gcgagctacg ccaccgcggt ggccgagcac ttccgcgacc gcggccagca cgtgctcctg
     5821 ctgatggact cgctcacccg ctacgcgatg gcgcagcgcg agatcgcgct ggcgatcggc
     5881 gagcccccgg ccacgcgcgg ctatccgccg agctgcttcg ccaagctgcc gcagctggtc
     5941 gagcgctcgg gcaacggcgc gcccggcggc ggctcgatca ccgccttcta caccgtgctc
     6001 accgagggcg acgatccgca ggatccgatc gccgatgcgg cacggggtat cctggacggg
     6061 cacatcgtgc tgtcgcgcga gctcgccgag tccggccact tcccggccat cgacgtcgag
     6121 aagtcgatct cgcgcgtgat gaccagcgtc gccccgcgcg accacatcgc cgcggcccgc
     6181 gtcgcgcgcc aggccctgtc gcgcatgacc aaggcgcgcg acctgatcca gctcggcgcc
     6241 taccagccgg gcgccgatcc cgagctcgac gccgccgtga agatggcccc gaagctgacc
     6301 gcgctgctgc agcaggacat gcacgagcgc gcctcgatgg ccgagagcaa gcacacgctg
     6361 aacaaggcga tcaccctctg acgcctaggg agccccgatg aagcgcctcc agatgctgtt
     6421 caccctgctc gatcgcgagc agaagcgccg cgacgacgcg cgcgccgtcg tgcagggcgc
     6481 cctgcgcaac gtcgaggcgc agcagcagca ggccgacggc ctcgctagct accgcgtcga
     6541 gtactgcgcc aagtggtcgg ccaagttcca gaccgcggcg accatcgaga tcctgcgcag
     6601 ctaccacggc ttcctcgcgc gcctggacca ggcggtcgcg cagcagcagc aggtcgtcgc
     6661 gcatgcgcag cgccaggccg agctcgcgcg ccagaagctc gtcgagcgcg agatccgcgt
     6721 cgcgacggtg gagcgcctga tccagcggcg ccaggcgctg ctcgcgaaga tcgccgaccg
     6781 gcgcgaccag aagaacctcg acgagatggc ggcgcggcgc gcgctgcccc gggagctggc
     6841 cggcatggcg tgacgccggc tcgaactcga tccccccgca agacatcccc cgcgaaccga
     6901 agacccacca tggccgtcaa cgcagcccat tcccacgccc acccctccaa ggcctcgcac
     6961 tccgcggcca acgcgatcgc cgcgccgcgc gcgggcgcgc atgtccgcgc gcgagccgat
     7021 gccgacgcgg atgccggcgg cgagggcttc gccgcgcagc tgatgcgcgc ccagcctcag
     7081 gagcacgacg ccgcgccgaa gtccgactcg aaggacgcgt cggccaagga caaggacgac
     7141 aagtccgcca aggacgacag gaaggacgcc gcggccgacg gcacgcagaa gcagcccgac
     7201 ccgtcgacgc tgccctggcc gccgcaggcg cagacgaccg ccgcgctggc cgcgcagtcg
     7261 gcgcctgccg agtccg
//
LOCUS       DASVLN010000152         5213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_115069, whole genome shotgun sequence.
ACCESSION   DASVLN010000152 DASVLN010000000
VERSION     DASVLN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115069"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VF453_03505"
     CDS             complement(<1..1479)
                     /locus_tag="VF453_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7686744.1"
                     /translation="MSEQIRGPRLRLSALAIGIGAALAGCAVGPDFHAPDAPVVADAS
                     HPYTPVALPAQTASAPGPAGDVQRFVAGQDIARSWWETFHSPTMNALVASALEHSPNL
                     AAAQATLREAEENYRATYGQLALPSVTGQLAATRERTPTATANGFSDPIVSLYNAGLD
                     VSYTLDVFGGNRRQIEGARAAVDVQRFQVEATWLSLTSSVVATAIQAASLEAQLKATQ
                     EVVDVETRSLGVIDKQVQLGALARSAVLQQRTLVAQTQAQLPPLQKALAQSRHQLAVL
                     TGRLPGDPDLPSVDLATLNLPHELPVSVPSDLVRQRPDIRASEATLHQASAEIGVATA
                     ALYPQVTLSGSIGREALTPGKLFTSGGLTAWTLAGSLTQPIFNGGALRAKRRAAVDAY
                     DAASAQYRQTVLQAFLDVANALRAVDTDAEALRADAQAEALARESLDLVERQYQLGAA
                     SYLASLDAQRTWLQTRVALAQAQAARYADTTALFQALGGGWWN"
     gene            1915..3522
                     /locus_tag="VF453_03510"
     CDS             1915..3522
                     /locus_tag="VF453_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7686745.1"
                     /translation="MPPCLSLRPSCPHFTRFTLAVALALPAATPAGAQTLRWASQGDP
                     QTMDPYSQNEGLTNMVNGQVYEFLVARDRRMNLVPGLATEWKQDSPTKWTFKLRRGVK
                     FHDGRPFTADDVVFSIQRAREKTSQIAVYAAPLGEVRKLDDHTVEFDLPRFDPVFLQH
                     VNLVYIMSRSWCVEHHAEHPQNFTAGEETWTALHANGTGPYVLESRQPDVKTVLRRNP
                     DYWQPIAGNVQQVVYTPIRNAGTRVAALLSHEVDFVLDPPPSDLGRLRADGQIQIVDG
                     MENRIVFIGMDQARDQLLYSSVKGRNPFKDERVRRAMYEAIDIEAIRTRLMNGQALPT
                     GAMAPTPKADFDDPALEKRLPYDVAGAKRLLAEAGYPDGFEVTLDCPNNRYINDERIC
                     IALASMWAKIDVKVRVNAMPRATYFPKLDKLDTSLYMLGWGGPVDDAETIFTPVMRNR
                     GADGVGAFNYGNSRDDVFDRLAAASTQEPDPARRGALIRQAFAREHEQVHLIPLHRQV
                     IPWAMWKGVSVVHRPDNWLEVSWITMK"
     gene            complement(3544..3915)
                     /locus_tag="VF453_03515"
     CDS             complement(3544..3915)
                     /locus_tag="VF453_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX7686746.1"
                     /translation="MTDALSPAAIATANITATTAAGPFRVDRFAVPRAALAAFLERVR
                     RSQRLLDEQPGCLQNLVLSRDDGAQADVDIVTIVEWADEAAMHAAKATVQARYAAEGF
                     DPAAFMRGLGVRADMVSYLPV"
     gene            4002..4502
                     /gene="soxR"
                     /locus_tag="VF453_03520"
     CDS             4002..4502
                     /gene="soxR"
                     /locus_tag="VF453_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HEX7686747.1"
                     /translation="MKNSAEGREGPLHPSGLPAELSVGEVARRSGVAVSTIHFYEAQG
                     LVASRRTAGNQRRYPRAALRRVAFVRVAQRVGISLADIAAALAELPEGSAPTRDDWAR
                     LSARWRAELDERMAQLKRLRDTLDDCIGCGCLSIDRCRLRNPADRLAAEGAGPRRLLA
                     GRARGG"
     gene            4531..5001
                     /locus_tag="VF453_03525"
     CDS             4531..5001
                     /locus_tag="VF453_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7686748.1"
                     /translation="MSVREVTQSIRIHAGPERVWDALVHPEAGERWRNARFATDWRPG
                     SPIAIEALIGTKRYRDKGRVLRAEPHSALEYTYWSRVSGLPDAPGSYSRIAMVLVADG
                     ADTVLTVTQRVPPSPVRRGPGWEIGDDSGARHVEFYWRTTLPVLKRVAEAGGAG"
BASE COUNT          730 a         1875 c         1897 g          711 t
ORIGIN      
        1 gttccaccag ccgccgccca gcgcctggaa cagcgccgtc gtgtcggcat agcgcgccgc
       61 ctgcgcctgc gcgagcgcca cccgggtctg cagccaggtg cgctgcgcgt cgagcgaggc
      121 gaggtagctc gccgcgccca gctggtactg gcgttcgacg aggtcgagcg actcgcgggc
      181 gagcgcctcg gcctgggcgt cggcgcgcag ggcctcggcg tcggtgtcca ccgcccgcag
      241 cgcgttggcg acgtcgagga aggcctgcag cacggtctgg cggtactgcg cggacgcggc
      301 gtcgtaggcg tcgaccgccg cgcggcgctt cgcccgcaac gcgccgccgt tgaagatcgg
      361 ctgcgtcagg ctgcccgcca gcgtccaggc ggtcagcccg cccgaagtga acagcttgcc
      421 cggcgtgagc gcctcgcgcc cgatgctgcc gctcagcgtg acctgcgggt agagcgccgc
      481 ggtcgcgacg ccgatctcgg cgctcgcctg gtgcagcgtc gcctcgctgg cgcggatgtc
      541 ggggcgctgg cgcacgaggt cggacggcac gctcaccggc agctcgtgcg gcaggttcag
      601 cgtggcgagg tcgacgctcg gcaggtccgg gtcgcccggc aggcggccgg tcagcacggc
      661 gagctggtgg cgcgactgcg caagcgcctt ctgcagcggc ggcagctgcg cctgcgtctg
      721 cgcgacgagc gtgcgctgct gcagcaccgc cgagcgcgcg agggcgccga gctgcacctg
      781 cttgtcgatc acgccgagcg agcgggtctc gacgtcgacc acttcctgcg tcgccttgag
      841 ctgggcctcg agcgaggcgg cctggatggc ggtggcgacg acgctggagg tcaggctcag
      901 ccaagtggcc tcgacctgga agcgctgcac gtcgaccgcg gcgcgcgcgc cttcgatctg
      961 gcgccggttg ccgccgaaca cgtccagcgt gtaggagacg tccaggccgg cgttgtacag
     1021 gctgacgatg ggatcgctga agccgttggc ggtcgcggtc ggcgtgcgct cgcgcgtcgc
     1081 ggcgagctgg ccggtcaccg aaggcagcgc cagctggccg taggtggcgc ggtagttctc
     1141 ctcggcctcg cgcagggtcg cctgggcggc ggcgaggttg gggctgtgct ccagcgccga
     1201 cgcgacgagc gcgttcatgg tgggcgagtg gaaggtttcc caccacgaac gcgcgatgtc
     1261 ctggcctgcc acgaagcgct gcacgtcgcc ggcggggccg ggtgcgctcg ccgtctgcgc
     1321 gggcagggcg accggcgtgt agggatggct cgcgtcggcc accaccggcg cgtcgggcgc
     1381 gtggaagtcg ggtccgaccg cgcagcccgc cagggccgcc ccgatgccga tcgccaaggc
     1441 gctcaggcgc aggcgcggcc ctcggatctg ctcactcatt cgtgctcctc gatgttttgg
     1501 ctcaggcagc cgcccggcac cggcgccagc cttcgcgggg gatggtagtc gggtttcccc
     1561 tcgggtaccc cgatgtcccc acaaagtagt tgccgttgca accattgact tgcccgctca
     1621 tctttcctgt gaacaaaaag acgggcgcat gtcgctcggg cgcggcgcag agtgtgaacc
     1681 ggttccaccc cgtccggggc cgcgatcgga tcgaacgacg ggagcggtcg gccaaacgcc
     1741 ggctggcgcc gacgaaatgc aggacgcggg ccaatgcgag gatgacctca tcgcccggcc
     1801 ggctccggga aaccgctgtc ccggcgcgac ggccggcatg cacgagccgt gccacactcg
     1861 gcccggatcc ggcgacccgc ccgggcacgc tcgcggacca ctaggagccg aaccatgccg
     1921 ccctgcctga gcctgcgccc gtcctgcccg cacttcacgc ggttcacgct cgccgtcgcg
     1981 ctggcgctcc ccgcggccac gccggccggc gcgcagaccc tgcgctgggc cagccagggc
     2041 gatccgcaga cgatggatcc gtactcgcag aacgagggcc tgaccaacat ggtcaacggc
     2101 caggtctacg agttcctcgt cgcccgcgac cgccggatga acctcgtgcc cggcctggcc
     2161 accgagtgga agcaggacag cccgacgaag tggacgttca agctgcgccg cggcgtcaag
     2221 ttccatgacg gccgcccgtt caccgccgac gacgtcgtct tctcgatcca gcgcgcgcgc
     2281 gagaagacct cgcagatcgc cgtctacgcc gcgccgctgg gcgaggtgag gaagctcgac
     2341 gaccacaccg tggagttcga cctgccgcgc ttcgacccag tcttcctgca gcacgtcaac
     2401 ctcgtctaca tcatgagcag gtcgtggtgc gtcgagcacc acgccgagca cccgcagaac
     2461 ttcaccgcgg gcgaggagac ctggaccgcg ctgcacgcca acggcaccgg cccgtacgtg
     2521 ctcgagagcc ggcagcccga cgtgaagacc gtcctgcggc gcaatccgga ctactggcag
     2581 ccgatcgcgg gcaacgtgca gcaggtcgtg tatacgccga tccgcaacgc cggcacgcgc
     2641 gtcgcggcgc tgctgtcgca cgaggtcgac ttcgtgctcg acccgccgcc ctccgatctc
     2701 ggccgcctgc gcgccgatgg ccagatccag atcgtcgacg gcatggagaa ccgcatcgtc
     2761 ttcatcggca tggaccaggc gcgcgaccag ctgctgtatt ccagcgtgaa gggccgcaac
     2821 cccttcaagg acgagcgcgt gcgccgggcg atgtacgagg cgatcgacat cgaggcgatc
     2881 cgcacccggc tgatgaacgg ccaggcgctg cccaccggcg cgatggcgcc cacgcccaag
     2941 gccgacttcg acgaccccgc gctcgagaag cgcctgccct acgacgtcgc gggcgcgaag
     3001 cggctgctcg ccgaggccgg ctacccggac ggcttcgagg tgacgctgga ctgcccgaac
     3061 aatcgctaca tcaacgacga gcgcatctgc atcgcgctgg cgtcgatgtg ggcgaagatc
     3121 gacgtcaagg tgcgcgtcaa cgcgatgccg cgcgcgacct atttccccaa gctcgacaag
     3181 ctcgacacca gcctctacat gctgggctgg ggcggcccgg tcgacgacgc cgagaccatc
     3241 ttcacgcccg tgatgcgcaa ccgcggcgcc gacggcgtgg gcgcgttcaa ctacggcaac
     3301 tcgcgcgacg acgtcttcga ccgcctcgcc gccgcctcga cgcaggagcc cgatccagcc
     3361 cggcgcggcg cgctgatccg gcaggccttc gcgcgcgagc acgagcaggt gcacctgatc
     3421 ccgctgcatc gccaggtgat cccatgggcg atgtggaagg gcgtgagcgt cgtgcatcgg
     3481 ccggacaact ggctcgaggt gtcgtggatc acgatgaagt gacgggccgc gccgcacgcc
     3541 gcgtcacacc ggcaggtagc tgaccatgtc ggcgcgcacg cccagcccgc gcatgaaggc
     3601 ggccgggtcg aagccctcgg ccgcgtagcg cgcctgcacc gtcgccttgg cggcgtgcat
     3661 ggcggcctcg tcggcccatt cgacgatggt gacgatgtcg acgtccgcct gggcgccgtc
     3721 gtcgcggctc agcaccaggt tctgcaggca gccgggctgc tcgtcgagca ggcgctgcga
     3781 ccggcgcacg cgttcgagga aggccgcgag cgcggcccgc ggcacggcga agcggtcgac
     3841 gcggaacggc cccgcggcgg tcgtggcggt gatgttggcg gtggcgatcg cggcggggga
     3901 cagggcatcc gtcatggcgg tactctccgg gaggcgcgcg gggattcgcg cggttcggtg
     3961 atactctgca acctcaacat agcttgaggt caagtgccgc gatgaagaat tccgccgaag
     4021 gccgcgaggg cccgctccat ccgtcgggcc tgccggccga gctcagcgtg ggcgaggtcg
     4081 cgcggcgcag cggcgtcgcg gtgtcgacca tccacttcta cgaggcccag ggcctggtcg
     4141 cgagccggcg cacggccggc aaccagcgcc gctacccgcg cgccgcgctg cgccgcgtcg
     4201 ccttcgtgcg ggtggcgcag cgcgtgggca tctcgctggc cgacatcgcg gccgcgctcg
     4261 ccgaactgcc cgagggatcc gcgccgacgc gcgacgactg ggcgcgcctg tccgcgcgct
     4321 ggcgcgccga gctcgacgag cgcatggcgc aactcaagcg cctgcgcgac acgctcgatg
     4381 actgcatcgg ctgcggctgc ctgtcgatcg accgctgccg cctgcgcaat ccggcggatc
     4441 gcctggccgc cgaaggcgcg ggcccgcggc ggctgctcgc gggccgggcc cggggcggtt
     4501 gaacacggcg cgcatcggca agccgggatc atgtccgtcc gcgaagtcac gcagtcgatc
     4561 cgcatccacg ccggacccga gcgggtgtgg gacgcgctcg tccatcccga ggccggcgag
     4621 cgctggcgca acgcgcgctt cgcgaccgac tggcggcccg gcagcccgat cgcgatcgag
     4681 gcgctgatcg ggacgaagcg ctaccgcgac aaggggcggg tgctgcgcgc cgagccgcat
     4741 tcggcgctcg agtacacgta ctggtcgcgg gtctccggcc tgccggacgc gccggggtcg
     4801 tattcgcgga tcgcgatggt gctcgtggcc gacggtgccg acacggtgct gaccgtcacg
     4861 cagcgggtgc cgccatcgcc ggtgcgccgc ggcccgggat gggagatcgg cgacgactcg
     4921 ggcgcccgcc acgtggagtt ctactggcgc acgacgctgc ccgtcctgaa gcgggtcgcc
     4981 gaggcgggcg gcgcgggctg aaggccatgc ggcgcttgcc ggacaatgcg cgcctgaccg
     5041 agaaaggacg atcccgatga cgtggctgcg cgccctggtt tggatgcttc cggcgctgct
     5101 tgcgctgccc gccgccgcgg cgccgccggt cgcgttcacc gacctcgcga cgccgttcga
     5161 gcgcgtcgcg gccgacaccg cgggccggcc cgatgccgag cgcatcgccg cgg
//
LOCUS       DASVLN010000153         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_330176, whole genome shotgun sequence.
ACCESSION   DASVLN010000153 DASVLN010000000
VERSION     DASVLN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330176"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..890)
                     /gene="cysT"
                     /locus_tag="VF453_03530"
     CDS             complement(57..890)
                     /gene="cysT"
                     /locus_tag="VF453_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX7686749.1"
                     /translation="MIARTLLLRRHSVLPGFDLALGFAVLYLCLIVLVPLSAVFLRTF
                     AGTWNGFVAAAFSPRVLASYRLTFGMSFAAALLNAGFGLLVAWVLVRYRFPARRLVDA
                     LVDLPFALPTAVAGISLTTLYAGNGWIGRWLPFKVSYTPLGVFVALTFIGLPFVVRTV
                     QPVLEDLHLELEEAAATLGASRWQTFRLVVLPALVPALATGFALAFARALGEYGSVIF
                     IAGNMPMVSEITPLLIITKLEQYDYTGATSIAVVMLVAAFVLLLLINLLQAWARRRQG
                     R"
     gene            complement(1094..1312)
                     /locus_tag="VF453_03535"
     CDS             complement(1094..1312)
                     /locus_tag="VF453_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX7686750.1"
                     /translation="MTILAVNVRNQFRGKVREIVEGPVVSEVDVETPQGLIVTSVVTT
                     RSIHELGLKVGKDVVALVKSTEVSIATL"
     gene            complement(1740..1994)
                     /locus_tag="VF453_03540"
     CDS             complement(1740..1994)
                     /locus_tag="VF453_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEX7686751.1"
                     /translation="MLNRLKRAEGQLRGIQRMIESGQPGIDIASQLVAVRRALDSTYV
                     HLTVSLVEQELGSRVGSDARGRKHVAEVLGELTALLSKVR"
     gene            complement(2269..2865)
                     /locus_tag="VF453_03545"
     CDS             complement(2269..2865)
                     /locus_tag="VF453_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7686752.1"
                     /translation="MVHLVDDEAPVRDALAFLFSSHGLAVRAYASGPEFLAAADEAPL
                     AGCVLLDVRMEPMSGLQVHDALVARGSGVPVLFLTGHGDIPMAVEALKKGAFDFIEKP
                     SGDAALVERVTRALAVDAARQAADSEEDEVAARLASLSEREREVMQRVAAGKLNKVIA
                     DELCISVRTVEVHRARVFAKLGVRSAAEVATLLARGRA"
BASE COUNT          457 a         1057 c         1115 g          364 t
ORIGIN      
        1 gcgcagccgg ggggctcgcg cgcacggggg cggaggtcgg cgcaacggcg ctcatctcaa
       61 cgcccctggc gccggcgcgc ccaggcctgc agcaggttga tcagcagcag cagcacgaag
      121 gccgcgacca gcatcacgac ggcgatcgag gtcgcgcccg tgtagtcgta ctgctcgagc
      181 ttggtgatga tcagcagcgg cgtgatctcg gaaaccatgg gcatgttgcc cgcgatgaag
      241 atcaccgacc cgtactcgcc cagcgcgcgc gcgaacgcga gcgcgaagcc ggtcgccagc
      301 gccggcacga gcgcgggcag cacgacgagc cggaaggtct gccagcgcga ggcgccgagc
      361 gtggcggcgg cctcctcgag ctcgaggtgc aggtcctcca gcaccggctg gacggtgcgc
      421 acgacgaagg gcaggccgat gaaggtgagc gcgacgaaca cgcccagcgg cgtgtagctc
      481 accttgaacg gcagccagcg gccgatccag ccgttgcccg cgtacagcgt cgtcagcgag
      541 atgcccgcga ccgcggtcgg cagcgcgaac ggcaggtcga ccagcgcgtc cacgagccgg
      601 cgcgccggga agcggtagcg caccagcacc cacgcgacga gcaggccgaa gcccgcgttc
      661 agcagcgcgg cggcgaagga catcccgaag gtgagccggt acgaggccag gacgcgcggc
      721 gagaaggcgg cggcgacgaa gccgttccac gtgcccgcga acgtgcgcag gaagaccgcg
      781 gacagcggca ccaggacgat caggcagagg tacagcaccg cgaagccgag cgccaggtcg
      841 aagccgggca gcacgctgtg gcggcgcagc aggagggtgc gggcgatcat gacggacggg
      901 aagcgtcgac cggcgcacgc ggacggtgcg acgccggtcg ggcggctgga gaaccgggcc
      961 ggctgcgcgc cggatggaac gcggcgccgt cggcccgggc gacggggttc gtccactgta
     1021 ggaggacgca cccccggcgc ggaacgaagc gatgcgcgga tcgatatgcg cgatccggtg
     1081 atgccccgcc gcttcagagc gtcgcgatcg acacctcggt ggacttcacc agcgccacga
     1141 catccttgcc gaccttcagg ccgagctcgt ggatcgagcg cgtggtgacg accgaggtca
     1201 cgatcaagcc ctggggcgtc tcgacgtcga cctcggacac gacgggtcct tcgacgatct
     1261 cgcggacctt gccgcggaac tggttgcgca cgttgacggc gaggatggtc atgggttcca
     1321 ccggggatgc tgcggacgac ggccgccgat gacccatctt cccggcgcgc cggccgccgg
     1381 ccaacgaatc ggaacgcgca tcgctcttcg gtttgtgcat gacgtgcgcg acaccggcgc
     1441 gtgtgggcgc ccacatacgc cagggcgtac gctgctcccg gcatgcgccg cacgcgcagc
     1501 cttcccggcc cggctcggca tggcacgccg cggctgcgcc gccccaagac cgcgccgcgg
     1561 ccttgcaagg tggccgcctc catgccgcgg gcagcgtccc gcacgggcgg gacgccgagg
     1621 cgtcgcctcg cgcacgcggg tgcgcaggcg caggtgcgcg cgccggggcg tccggcacgc
     1681 gagaaccggt ccatcccctc gcgccggcgc tgccggcgcg acggggctgg gcgcgcgctt
     1741 cagcgcacct tggacagcag cgcggtcagc tcgccgagga cttcggcgac gtgcttgcgg
     1801 ccgcgggcgt cgctgccgac gcgggagccc agctcctgct cgacgaggct cacggtcagg
     1861 tgcacgtagg tgctgtccag cgcgcggcgc accgcgacca gttggctcgc gatgtcgatc
     1921 cccggctgcc cggactcgat cattcgctgg atccctcgca gctgcccttc ggcacgcttg
     1981 aggcggttga gcatttcgct tcggctttgt tcgtcgacaa gcactgacat tgtatggacc
     2041 ctccaacggc agcccggatg cggccaccgt cgcgcgattg tgacggctcg gccgcatcct
     2101 tgcataggtg ggcgggtacc caggacacgg taaagatgac ggattcccgc gctttatccc
     2161 tagataaccg gggcctgcag ccctcccgcg cccgcacgcg ggcgacaaac gccatcgata
     2221 cggttcacgg tatgcagcgc cgccatcgcc gcggcgcggg ccgtccgctc aggcgcgccc
     2281 gcgcgcgagg agggtcgcga cctcggccgc ggagcgcacc cccagcttgg cgaacacgcg
     2341 cgcgcggtgg acttcgacgg tgcgcaccga gatgcacagt tcgtcggcga tgaccttgtt
     2401 caacttgccg gcggccacgc gctgcatcac ctcgcgctcc cgctcggaca ggctcgccag
     2461 gcgcgccgcg acctcgtcct cctcggaatc ggcggcctgg cgcgcggcgt ccacggccag
     2521 cgcgcgcgtg acgcgctcca ccagcgccgc atcgccggag ggcttctcga tgaagtcgaa
     2581 ggcgcccttc ttcagcgcct cgaccgccat cgggatgtcg ccgtggccgg tgaggaagag
     2641 gacgggcacg ccgctgccgc gcgccaccag cgcgtcgtgc acctgcaggc cggacatcgg
     2701 ctccatgcgc acgtccagca ggacgcagcc ggccagcggc gcctcgtcgg cggcagccag
     2761 gaactcgggc ccgctcgcat aggcgcgcac cgcgaggccg tgcgacgaga acaggaaggc
     2821 gagcgcgtcg cgcaccgggg cctcgtcgtc gacgagatgc accatgggct cggcggcggc
     2881 gaggcgggag ggggaggcga gggtggcgtt catgggcggt acgggtgggc ggcgggcggc
     2941 gcggcggcgt cctcgcgcgc ggcgtcggcg tcggggccgg ccggcgaggt cgc
//
LOCUS       DASVLN010000154         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_115104, whole genome shotgun sequence.
ACCESSION   DASVLN010000154 DASVLN010000000
VERSION     DASVLN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115104"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VF453_03550"
     CDS             complement(<1..704)
                     /locus_tag="VF453_03550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7686753.1"
                     /translation="MRIAMISEHASPLATIGGVDSGGQNVYVAQVARELARLGHEVDV
                     FTRRDDAALPTVVQVAPRWRVIHVDAGPPAFVPKEQLLGHMAAFAAFCDAWLRTGPGY
                     DVVHANFFMSGQVGLALKRKFGLPLVTTFHALGLVRREHQREADAFPPERIAIERALV
                     EHSDRVVAECPQDELDLRRLYGADPGRMATIPCGFDAGEFAPQPREHARARLGLPRDE
                     FMVLQLGRMVPRKGVET"
     gene            complement(1114..>2878)
                     /locus_tag="VF453_03555"
     CDS             complement(1114..>2878)
                     /locus_tag="VF453_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEX7686754.1"
                     /translation="LALAGGTRAATPDFGPDVRVFTPDMAVADIQAAVDAVAARQLSN
                     QFGPERVALLFMPGTYGSAAAPLNFQLGYYTSVAGLGRAPTDVAINGSINAYNQCDAS
                     GCVALNNFWRSISNLDIEVNNASLGCHAGEFWAVSQAAPMRRVRVNGNTTLMDYCSGP
                     SYASGGFIADSQFVGGTIISGSQQQWITRDSTIDGWTNGVWNQVFSGVVGAPATCFPG
                     STACGGPYTTLPTSPVTREAPYLFVDAQGAWQVLAPTLRRESSGTTWANGPTPGTAIP
                     ISKFFIAKPTDSTARINEALLRGANLILTPGVYKLDDALRIPYANTIVLGLGFPTLIP
                     QHGTAAIKVDDQPGVDITGVLIDAGPVNSPVLLQLGTSARQLVSRLGGAPVALHDVFF
                     RIGGAQAGRATTSLVVNSNGAILDDIWAWRADHGNGVGWNVNKADTGVVVNADDVTAY
                     GLFVEHYQKTETLWNGERGRVVFYQNEMPYDPPSQAAWSESNTVLGYPALKVADGVKQ
                     FNGYGLGSYNFFNQGLDIYASNAFEVPASLPSGSLQDLLTVFLDPSHGRGGVLNVVDG
                     AGGSATAANPSTPVTVTRFPQ"
BASE COUNT          407 a         1014 c         1050 g          407 t
ORIGIN      
        1 gtctccacgc ccttgcgcgg caccatgcgg cccagctgca gcaccatgaa ctcgtcgcgc
       61 ggcaggccca gccgcgcacg cgcgtgctcg cgcggctgcg gcgcgaactc gccggcgtcg
      121 aagccgcagg ggatggtggc catgcgcccc ggatcggcgc cgtagaggcg gcgcaggtcg
      181 agctcgtcct gcgggcactc ggccacgacg cggtcgctgt gctcgacgag cgcgcgctcg
      241 atggcgatgc gctcgggcgg gaaggcgtcg gcctcgcgct ggtgctcgcg ccgcaccagg
      301 ccgagcgcgt ggaaggtcgt gaccagcggc agcccgaact tccgcttcag cgcgagcccg
      361 acctggcccg acatgaagaa gttggcatgc acgacgtcat accccggccc cgtacgcagc
      421 cacgcgtcgc agaacgcggc gaaggccgcc atgtggccga gcagctgctc cttgggcacg
      481 aaggccggcg gcccggcatc gacatggatg acgcgccagc gcggagcgac ctgcacgacc
      541 gtcgggagcg ccgcgtcgtc gcgccgcgtg aagacgtcga cctcgtgccc cagccgggca
      601 agctcgcgcg cgacctgcgc gacgtagacg ttctggccac ccgagtccac gcccccgatg
      661 gtcgcgaggg gcgaggcgtg ttcactgatc atggcgatgc gcatccggcg gtttcctccg
      721 ggtcgtggcg gaggaagagg cgagcggcgt gcgaggccgc gaacgcggcc atggaagcgg
      781 ccgcccagcg tccccgggaa gcgcttctcc ctctgtggaa ccgcttctcg cggaggtcgc
      841 gaggccgggc aggcgcaccg cgcgcgccac ctgccgttcg gacggaaacg ccggtgctct
      901 ttacggcaag gagcttcgat ccggttgaag cccgggccgg tcgcggcttc tacgcgattg
      961 aatccggcgc cgccgaggct cgacggcccc gcgagctcgg cggccgttca gcgcgacggc
     1021 gcgccttgtg cggcaccgtc aggccgcgca cgcgtacgca ggcgcgcgaa gaagcgggac
     1081 ggccggccca cacgccggcc gtcccgggcc cgctcactgc gggaagcgcg tcaccgtcac
     1141 cggcgtgctc gggttggcgg cggtcgcgga gccgcccgcg ccgtccacca cgttcagcac
     1201 gccgccacgg ccgtgcgagg ggtcgaggaa gaccgtcagc aggtcctgca ggcttcccga
     1261 gggcaggctc gcgggcacct cgaacgcgtt ggaggcgtag atgtcgagcc cctggttgaa
     1321 gaagttgtac gagcccagcc cgtagccgtt gaactgcttc accccgtcgg cgaccttcag
     1381 cgccgggtac ccgagcaccg tattcgactc gctccatgcg gcctgcgacg gcgggtcgta
     1441 gggcatctcg ttctggtaga acacgacccg gccgcgctcg ccgttccaca gggtctccgt
     1501 cttctggtag tgctcgacga acaggccgta ggccgtcacg tcgtcggcat tgacgaccac
     1561 gcccgtgtcg gccttgttga cgttccagcc gacgccgttg ccgtggtcgg cgcgccaggc
     1621 ccagatgtcg tcgaggatcg cgccgttcga attcacgacg agcgacgtgg tcgcgcgccc
     1681 ggcctgggcg ccgccgatgc ggaagaacac gtcgtgcagc gccaccggcg cgccgcccag
     1741 gcgcgagacc agctgccgcg cgctcgtgcc cagctgcagc agcaccggcg agttcaccgg
     1801 cccggcatcg atcagcacgc cggtgatgtc cacgcccggc tggtcgtcga ccttgatcgc
     1861 cgccgtgccg tgctgcggga tcagcgtcgg gaagcccagg cccagcacga tcgtgtttgc
     1921 gtacgggatg cgcagcgcgt cgtcgagctt gtagacgcct ggcgtcagga tcaggttggc
     1981 gccgcgcagc agcgcctcgt tgatgcgcgc cgtcgagtcc gtcggcttgg cgatgaagaa
     2041 cttcgagatc gggatcgcgg tgcccggcgt cggcccgttc gcccacgtcg tgccggagga
     2101 ctctcggcgc agcgtcggcg cgagcacctg ccaggcgccc tgggcgtcga cgaacaggta
     2161 cggcgcctcg cgcgtgacgg ggctcgtcgg cagcgtcgtg tacgggccgc cgcaggcggt
     2221 cgagccggga aagcacgtcg ccggcgcgcc gacgaccccc gagaacacct ggttccacac
     2281 gccgttcgtc cagccgtcga tcgtgctgtc gcgcgtgatc cactgctgct gcgagccgct
     2341 gatgatggtc ccgcccacga actgcgagtc ggcgatgaag ccgccgctcg cgtacgacgg
     2401 gccgctgcag tagtccatca gcgtcgtgtt gccgttcacg cgcacgcggc gcatcggcgc
     2461 ggcttgcgac accgcccaga actcgccggc gtggcagccg aggctcgcgt tgttcacctc
     2521 gatgtcgagg ttggagatcg agcgccagaa gttgttcagc gcgacgcagc ccgacgcgtc
     2581 gcactggttg tacgcattga tcgagccgtt gatggccacg tcggtcggcg cgcggccgag
     2641 gcccgcgacg ctcgtgtagt agccgagctg gaagttcagc ggcgcggccg cggagccgta
     2701 ggtgccgggc atgaacagca gcgcgacccg ctcggggccg aactggttcg acagctgacg
     2761 tgccgcgacg gcgtcgaccg ccgcctggat gtcggcgacg gccatgtcgg gcgtgaagac
     2821 gcgcacgtcg gggccgaagt cgggggtcgc ggcgcgggtg ccgccggcga gcgcgagc
//
LOCUS       DASVLN010000155         3799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_61151, whole genome shotgun sequence.
ACCESSION   DASVLN010000155 DASVLN010000000
VERSION     DASVLN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61151"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF453_03560"
     CDS             complement(<1..555)
                     /locus_tag="VF453_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686755.1"
                     /translation="MNSPLAAAVAVLAIVAVGASARAQPAAASAPASAAGGAISVGML
                     ADLMAARLKNQCDMARTALELSHAPQERDLLASNAELACDCLPAQLAPALGPDRDAPV
                     DQAQFLARMRSALDTCGARGIRRSIARSCERGFDPTDAASEPVASADARQRAHCDCMR
                     DGLARMDDASLARAAEDAYRRFGER"
     gene            complement(619..1074)
                     /locus_tag="VF453_03565"
     CDS             complement(619..1074)
                     /locus_tag="VF453_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7686756.1"
                     /translation="MVKRTSHMETGCPVARPLDALGDGWSLLIVRDAFDGLARFGEFQ
                     KSLGLAKNILASRLRHLVEHGILEASAAADGGAHQEYRLTAKGRALFPVLVALRQWGE
                     AHCFEPGELQVRLVDRKRGRPVRRLELRSHDGRLLDAEDTRVDFDMGDG"
     gene            1213..2139
                     /locus_tag="VF453_03570"
     CDS             1213..2139
                     /locus_tag="VF453_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7686757.1"
                     /translation="MIDADETFDGTFAFAPHFTDAPGFPMHFVDEGPRDGEVVLCLHG
                     EPTWGYLFRHLVPALRDRCRVVVPDHMGFGKSAAPPARSYRLQDHVDNLERLVLALDL
                     RGITLVMHDFGGPTGMGLAARHPGRIARVIAVNGPTPFGQADLGERLAANAATAPWFR
                     WIAQAHADGSLEPVLERLGFNILSVLKLNGFEDHALITDRWLRAYGAPFATPADCAGA
                     IGWARGFALGEHRFEEPDADARRILAGKPAMAIWGLADRTLQAAHFLPLFRAAFPGAP
                     VHELPGVGHYSLEDAPADVNGLVAAFIRGTRG"
     gene            complement(2157..2741)
                     /locus_tag="VF453_03575"
     CDS             complement(2157..2741)
                     /locus_tag="VF453_03575"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7686758.1"
                     /translation="MRQPVPLDRCTRLLNHGPTVLVSAKHEGRRNVMAAAWSMPLDFL
                     PPKVAVVLDKQTFTRGLVEASGRFALAVPPVALAALTQRVGNTSGRDVDKFAVPDALA
                     RAEPGPATGAPLVEGCVAWLECRRLPEAHVEQAYDLFLGEVVAAWADDRVFRDGRWHF
                     ADADPALRTLHHIAGGHFLAIGDEVGYAPDTRPR"
     gene            2875..3468
                     /locus_tag="VF453_03580"
     CDS             2875..3468
                     /locus_tag="VF453_03580"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEX7686759.1"
                     /translation="MSEALNDAALKQLFLEARTFNKFQPRPVDDATLHALVDLLKWAP
                     TSMNAQPGRFVFVRSAEAKARLKPALMPGNQDKTMAAPVTVIVAHAPDFHEHLPTQFP
                     AIPGARDMFAGNDAMREITALRNGTLQGAYLIVAARALGLDVGPMSGFENAAVDAAFF
                     PGGEWKSNFLINLGYGDPSGNYPRGPRLPFDTVARIV"
     gene            complement(3478..>3799)
                     /locus_tag="VF453_03585"
     CDS             complement(3478..>3799)
                     /locus_tag="VF453_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7686760.1"
                     /translation="YEQGSGARRAALAALEAMRRPYRIVCESASPAGMLAAVDSGLAV
                     ALFSRCSVPAHHELLGERHGLPPLPLQQVVLMRSRASAGSAAADAMAAQVLDTLGRSQ
                     RPAA"
BASE COUNT          521 a         1397 c         1367 g          514 t
ORIGIN      
        1 gcgctcgccg aagcggcgat acgcgtcctc ggccgcgcgg gcgaggctgg cgtcgtccat
       61 gcgtgccagg ccgtctcgca tgcagtcgca gtgcgcgcgt tggcgcgcgt cggccgaggc
      121 gaccggctcg gacgccgcgt cggtcggatc gaagccgcgc tcgcacgagc gcgcgatcga
      181 gcgccggatg ccgcgcgcgc cgcaggtgtc gagggccgat cgcatccgcg ccaggaactg
      241 cgcctgatcc acgggagcgt cgcggtcggg acccagcgcg ggcgcgagct gcgcgggcag
      301 gcagtcgcat gcgagttccg cgttgctcgc gagcaggtcg cgctcctgcg gcgcgtgcga
      361 cagctccagg gccgtccggg ccatgtcgca ctggttcttc aatcgcgcgg ccatcaggtc
      421 ggccagcatg ccgacgctga tggcgccgcc ggccgcggac gccggtgccg aggcagccgc
      481 cggctgggcg cgcgcggatg cgccgacggc gacgatcgcg aggacggcga cggcggcggc
      541 gagcggggag ttcatcgatg gtcggtctcg gggaagggcg caggcggcgc ccggcgcccg
      601 ccattgtgcc ggcggcgatc agccgtcgcc catgtcgaaa tcgacgcgcg tgtcctccgc
      661 gtcgagcagt cggccatcgt gcgagcgcag ctccaggcgc cggacgggcc ggccgcgctt
      721 gcgatcgacg agccgcacct gcagctcgcc cggctcgaag caatgcgcct cgccccattg
      781 gcgcaaggcc acgagcaccg ggaacagggc gcggcccttg gccgtcagcc ggtattcctg
      841 gtgcgcgccg ccgtccgcgg cggctgaggc ttcgaggatg ccgtgctcga cgagatgccg
      901 cagccgcgac gccaggatgt tcttcgcgag gccgaggctc ttctggaact cgccgaagcg
      961 ggcgaggccg tcgaacgcgt cgcggacgat cagcagcgac cacccgtcgc ccagcgcatc
     1021 caggggccgc gccacggggc atcccgtttc catgtgactc gttcgcttga ccatgcctgc
     1081 ctcgattgag tggttgcatc ataaaacctt gtttggtaca acgcgaatgg tttcaacatg
     1141 aaaccttctg aagaaccgat ccgccgcggc ctcccgccgc cgtggcgccc gcaactccac
     1201 caccttccga ccatgatcga tgccgacgaa accttcgatg gaaccttcgc gttcgccccg
     1261 cacttcaccg acgcgccggg gttcccgatg cacttcgtcg acgaggggcc gcgcgacggc
     1321 gaggtcgtgc tgtgcctgca cggcgagccg acctggggct acctcttccg ccacctcgtg
     1381 ccggcactgc gcgaccgttg ccgcgtcgtc gtgcccgacc acatgggctt cggcaagagc
     1441 gcggcgccgc cggcgcgcag ctaccggctc caggaccacg tcgacaacct cgaacgcctc
     1501 gtcctcgcgc tcgacctgcg cggcatcacg ctcgtgatgc acgacttcgg cggcccgacc
     1561 ggcatgggcc tggccgcgcg ccatccgggc cgcatcgcgc gcgtgatcgc cgtcaacggg
     1621 ccgacgccgt tcggccaggc ggatctcggc gagcgcctcg cggccaacgc cgcgacggcg
     1681 ccgtggttcc gctggattgc gcaggcgcac gcggacggca gcctcgagcc cgtgctggag
     1741 cggctcggct tcaacatcct cagcgtgctg aagctgaacg gcttcgagga ccacgcgctg
     1801 atcacggaca ggtggctgag ggcctacggc gcaccgttcg cgacgccggc cgattgcgcg
     1861 ggggcgatcg gctgggcgcg cggcttcgcg ctcggcgagc atcggttcga ggagcccgac
     1921 gccgacgcgc gccggatcct cgccggcaag ccggccatgg cgatctgggg cctggccgac
     1981 cggacgctgc aggccgcgca cttcctgccg ctgttccgcg ccgcgtttcc cggcgcgccc
     2041 gtccacgagc tgcccggcgt cgggcactac agcctggagg acgcgcccgc cgacgtgaac
     2101 gggctcgtcg cggccttcat ccgcggcacg cgcgggtgac gggggccggc cgggcctcag
     2161 cgcggtcggg tgtccggcgc gtagccgacc tcgtcgccga tcgcgaggaa atgcccgccg
     2221 gcgatgtggt gcagcgtgcg caacgcgggg tcggcgtcgg cgaagtgcca gcggccgtcc
     2281 cggaacacgc ggtcgtcggc ccaggcggcc acgacctcgc cgaggaacag gtcgtaggcc
     2341 tgctcgacat gcgcctcggg caggcggcgg cattcgagcc aggcgacgca gccctcgacc
     2401 agcggggcgc ccgtggcggg cccgggctcg gcgcgggcga gcgcgtccgg caccgcgaac
     2461 ttgtcgacgt cgcggcccga ggtgttgccc acgcgctggg tgagcgcggc cagcgccacc
     2521 ggcggcacgg cgagcgcgaa gcggccgctc gcctcgacca ggccgcgggt gaaggtctgc
     2581 ttgtcgagga cgacggcgac cttgggcggc aggaagtcca gcggcatcga ccaggcggcc
     2641 gccatcacgt tgcgccggcc ttcgtgcttg gcgctgacga gcaccgtggg cccgtggttc
     2701 aggaggcggg tgcagcgatc gagggggacg ggctggcgca tggccgcatt ctcgccccgg
     2761 acgggcgttg accgaatttg aacgggatct tcgacatcct tgcacggccg cggcgcgggc
     2821 tcgcgggatg atgccggcca gccgtccccc attcatccgc caggaacccc gaccatgtcc
     2881 gaagccctga acgacgccgc gctcaagcag ctgttcctcg aagcccgtac cttcaacaag
     2941 ttccagccgc gcccggtgga cgacgcgacg ctgcacgcgc tcgtcgacct cctgaagtgg
     3001 gcgccgacct cgatgaacgc ccagccaggc cgcttcgtct tcgtgcgcag cgccgaagcc
     3061 aaggccaggc tgaagcccgc gctgatgccg ggcaaccagg acaagaccat ggccgcgccg
     3121 gtcacggtga tcgtcgccca cgcgcccgat ttccacgagc acctgcccac gcagttcccg
     3181 gcgatcccgg gcgcgcgcga catgttcgcc ggcaacgatg cgatgcgcga gatcaccgcg
     3241 ctgcgcaacg gcaccctgca gggcgcctac ctgatcgtcg cggcacgcgc gctggggctg
     3301 gacgtcgggc cgatgagcgg cttcgagaac gccgccgtcg acgcggcgtt ctttcccggg
     3361 ggcgagtgga agtcgaactt cctgatcaac ctcggctacg gcgacccgag cggcaactat
     3421 ccgcgcggcc cgcgcctgcc cttcgatacg gtcgcgcgta tcgtctgacc cgtcccgtca
     3481 ggccgcggga cgctggctgc ggcccagggt atcgagcacc tgcgccgcca tcgcgtcggc
     3541 tgcggccgag cctgccgacg cgcggctgcg catcagcacg acctgctgca gcggcagcgg
     3601 cggcaggccg tggcgttcgc ctagcagctc atggtgcgcc gggacgctgc agcgcgagaa
     3661 cagggccacc gccagcccgc tgtcgacggc ggccagcatg ccggccgggc tcgcgctctc
     3721 gcacacgatg cgatagggtc gccgcatcgc ctccagcgcc gcgagcgcag cccgccgcgc
     3781 gccgctgccc tgctcgtag
//
LOCUS       DASVLN010000156         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_438162, whole genome shotgun sequence.
ACCESSION   DASVLN010000156 DASVLN010000000
VERSION     DASVLN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438162"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VF453_03590"
     CDS             complement(<1..1059)
                     /locus_tag="VF453_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7686761.1"
                     /translation="MLLHAVLAFSLFETLALAGVLLAWADRVAGARLLAVFLVGIATW
                     IAGNEAPIWLGPQAEPLMMALLATAPFTSAFFFHFCVVFCRVHVGRKTIAAAYALGAI
                     STLVAELGHPAHMEPGRHIAWIAVAEPLGWGASVAWIVLGSGGVAVLLAALARARPPA
                     FSQIAAVSASCLWGLACLAGYGMEVSGLAVYPFPLLGLPLYPVILVYGILRYGVFVAN
                     AWARRALVWTLLLAFGAGVVALTPLLPFESRWLGGLVVAASCLALNGPLRRLAERLVY
                     PGGTVTATDLARWRVALQAADSPGALAEQAATLLALRIGLPVAVRVGDDGMTAGSDLP
                     LLACTHGPQGWQATLVGWD"
     gene            1230..1895
                     /locus_tag="VF453_03595"
     CDS             1230..1895
                     /locus_tag="VF453_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEX7686762.1"
                     /translation="MTRIVVSAASPAFIPSAPGLDLVAHVGAAGWARLPTAVRRRFGA
                     GHGDVVYAGRITLRRSRAGRAFAALARLFGGPLTAAAGESLPAVVRVATDGRGGMTWE
                     RAFDAGGAHGMQVVRSTKETGPGGELVERTAGGLGMVLVAFEERGTLVFESRGFVFVL
                     GRLRLRVPALLAPGTCRVVHADLGGGRFRFALDMRHPLWGHTFHQSGVFVDPHDATEE
                     VAQ"
     gene            1892..>2647
                     /locus_tag="VF453_03600"
     CDS             1892..>2647
                     /locus_tag="VF453_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7686763.1"
                     /translation="MTAVLIILAVQALLGAFDNFWHHELQARLPQRASARRELALHAA
                     REAIYAVVFLGLGWFEWHGALACALAALLLAELAITLADFLEEDRSRRLPPFERVLHT
                     LLTMGYGLFLGAIAPQLWRWARLPIAFVASPHGTLSVLFTLFAIGVGAWAVRNGMAVA
                     ALGRSCRSASTVPADAPHDPRIAPAVLVAGGTGFVGRALVARLVRERRRVIVLARDLV
                     QARADFGPRVWVVDRLDAIPPETRLDAVVHLAGA"
BASE COUNT          356 a          970 c          965 g          356 t
ORIGIN      
        1 gtcccagccg accagcgtcg cctgccagcc ctgcgggccg tgcgtgcagg cgagcagcgg
       61 caggtcgctg cccgctgtca tcccgtcgtc gccgacgcgc accgccactg gcaagccgat
      121 gcgcagcgcg agcagcgttg cggcctgctc cgccagcgct ccgggcgagt cggccgcctg
      181 cagggcgacg cgccagcgcg cgaggtcggt tgccgtcacg gtgccgccgg gatacaccag
      241 ccgctccgcg agccgtcgca gcggcccgtt gagcgccagg caactggccg cgacgaccag
      301 cccgccgagc cagcgcgact cgaacggcag caggggcgtg agagccacca cgcccgcgcc
      361 gaaggccagc agcaacgtcc acacgagcgc gcgccgtgcc cacgcgttgg cgacgaatac
      421 cccgtagcgc aggatgccgt agacgaggat gaccggatac agcggcagcc cgagcagcgg
      481 gaacggatac accgccagcc cggatacctc catcccgtat ccggccaggc aggccagtcc
      541 ccacaggcac gatgccgaga cggccgcgat ctggctgaac gcggggggcc gcgcccgcgc
      601 cagcgccgcc agaagcaccg cgacgccacc cgagcccagc acgatccacg cgacgctcgc
      661 gccccaaccg agcggctcgg ccaccgcgat ccaggcgatg tggcggccgg gctccatgtg
      721 tgcgggatgg cccagctcgg cgaccagcgt ggagatcgcg cccagtgcgt acgcggccgc
      781 gatcgtcttg cggccgacgt ggacgcggca gaagacgacg cagaagtgga agaagaacgc
      841 ggacgtgaag ggcgcggtcg cgaggagcgc catcatcagc ggctcggcct gcggcccgag
      901 ccagatcggc gcctcgttgc ccgcgatcca ggtcgcgatg cccaccagga agaccgccag
      961 cagccgcgcg cccgccacgc ggtcggccca agccagcagc acgccggcca gcgcgagcgt
     1021 ctcgaacagg gagaaggcga ggacggcgtg cagcagcatg gcggcggcgc tgggcggggt
     1081 gtcaacgcgg ttgacgacag gggccgcggg cagtgtaaac gaagtacaca gtctcgatcg
     1141 ggaacctcag tcgaatcaac aacttgcgag cgccaggttc gttggcccgg ttctcgcgaa
     1201 gacgggttcg tcccaatgga ggagaaccca tgacacgcat cgtcgtttcc gcagcctcgc
     1261 cggcattcat tccgtccgct cccgggctcg acctggtcgc ccacgtcggt gcggccggct
     1321 gggcgcgcct gcccaccgcc gtgcgccgcc gtttcggcgc cggccacggg gacgtcgtct
     1381 atgccggccg catcacgttg cgccgctcgc gggccggacg cgccttcgcg gcgctcgcgc
     1441 gactgttcgg cgggcccttg acggctgcgg ctggcgagtc gctgcccgcg gtcgtgcgcg
     1501 tcgccacgga cggccgcggc gggatgacct gggagcgcgc gttcgacgcc ggcggcgcac
     1561 acgggatgca ggtcgtgcga tcgaccaagg agaccggccc cggcggcgag ctcgtggaac
     1621 gcaccgctgg cgggctgggc atggtgctgg tcgccttcga ggagcgcggc acgctggtct
     1681 tcgagagccg cggcttcgtg ttcgtgctcg ggcgcctgcg cctgcgagtg cctgcgttgc
     1741 tcgcacccgg aacctgccgg gtcgtgcacg ccgacctcgg cggtggccgg ttccgcttcg
     1801 cgctggacat gcgccacccg ttgtggggcc ataccttcca ccagtcgggg gttttcgtcg
     1861 acccgcacga cgccaccgag gaggtcgcgc aatgaccgcg gtgctgatca tcctggccgt
     1921 gcaggcgctg ctgggggcgt tcgacaactt ctggcaccac gaactgcagg cgcgcttgcc
     1981 gcagcgcgcg tcggcgcggc gcgagctggc gctgcatgcc gcgcgcgagg cgatctacgc
     2041 ggtcgtcttc ctcggcctcg gctggttcga gtggcacggc gcgctggcgt gcgcgcttgc
     2101 cgccctgttg cttgccgagc tcgcgatcac gctggccgac ttcctcgagg aggaccgctc
     2161 gcgccggctg ccgccgttcg agcgcgtgct gcatacgctg ctgacgatgg gctacggcct
     2221 gttcctcggc gccatcgcac cccagctgtg gcgctgggcc cgcctgccga tcgccttcgt
     2281 ggcctcgccg cacggcacgc tgtcggtgct gttcacgctc ttcgccatcg gcgtgggcgc
     2341 ctgggccgtg cgcaacggga tggcggtcgc cgcgctggga cgatcgtgcc gaagcgcgag
     2401 caccgtaccc gcggacgcgc cgcacgaccc tcgcatcgcc cctgcggtgc tcgtggcggg
     2461 agggacgggc ttcgtcggcc gcgcgctggt ggcccggctc gtgcgcgagc gccgacgtgt
     2521 catcgtgctg gcgcgcgacc tcgtgcaggc gcgcgcggac ttcgggccgc gcgtgtgggt
     2581 cgtcgaccgc ctcgacgcga tcccgcccga gacgcggctc gacgctgtcg tccatctcgc
     2641 cggcgcg
//
LOCUS       DASVLN010000157         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_8413, whole genome shotgun sequence.
ACCESSION   DASVLN010000157 DASVLN010000000
VERSION     DASVLN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8413"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..>1692
                     /gene="hrpA"
                     /locus_tag="VF453_03605"
     CDS             82..>1692
                     /gene="hrpA"
                     /locus_tag="VF453_03605"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEX7686764.1"
                     /translation="MTDGILLAETQTDPLLQAYDTLIIDEAHERSLNIDFLLGYLREI
                     LPRRPDLKVIVSGETGSGKTTQLPKIALALGRGRANGGGLVGHTQPRRIAASSVAKRI
                     AFELNSPLGEVVGYRVRFQDRLQPGASVKLMTDGILLAETQSDPLLRAYDTLIIDEAH
                     ERSLNIDFLLGHLRQILPRRPDLKIVVTSATIDAERFAQHFASARGPAPVLHVSGRTF
                     PVEQRYRPFEESREYGLNEAIADAVDELWRQGPGDVLVFLPGEREIRDAAEALRKHHP
                     PGVDILPLFARLSQAEQDRVFETGGAPRIVLATNVAETSLTVPGIRYVVDSGLARVKR
                     YSYRTKVEQLQIEPVSQAACNQRAGRCGRVSNGICIRLFDEADFLARPRFSDPEILRS
                     SLAGVILRMKSLRLGAVEDFPFLEEPPRKAIADGYALLGELNAVDDANELTPVGVELA
                     KLPLDPRVGRMILEARDRHCLAEVLVIASALSGQDVRDRPLEQQQAADEKHRKFDDEK
                     SEFMGYLKLWKWLETSRGGQAEAIPDAVS"
BASE COUNT          264 a          619 c          576 g          233 t
ORIGIN      
        1 ctgaagacgc cgctgggcga agtcgtcggc tacaaggtcc gcttccagga tcgcctgcag
       61 ccgggcgcca gcgtcaagct gatgacggac ggcatcctgc tggccgaaac ccagaccgat
      121 ccgctgctgc aggcctacga cacgctgatc atcgacgagg cccacgaacg ctcgctgaac
      181 atcgacttcc tgctcggcta cctgcgcgag atcctgccgc ggcggccgga cctgaaggtc
      241 atcgtcagcg gcgagaccgg ctccggcaag acgacgcagc tgcccaagat cgcgctcgcg
      301 ctcggccgcg gaagggccaa cggcggtggc ctcgtcggcc acacccagcc gcgccgcatc
      361 gccgcgtcga gcgtggccaa gcgcatcgcg ttcgagctga attcgccgct cggcgaagtg
      421 gtgggctacc gcgtgcgctt ccaggaccgg cttcagcccg gcgcctcggt caagctgatg
      481 accgacggca tcctgctggc cgagacccag tccgacccgc tgctgcgcgc ctacgacacg
      541 ctgatcatcg acgaggccca cgagcgcagc ctcaacatcg acttcctgct cggccacctg
      601 cgccagatcc tgccgcgccg gcccgacctg aagatcgtcg tcacctcggc cacgatcgac
      661 gccgagcgct tcgcgcagca tttcgcctcg gccaggggcc ccgcgccggt gctgcacgtc
      721 agcggccgca ccttcccggt cgagcagcgc taccgcccgt tcgaggagtc gcgcgaatac
      781 ggcctgaacg aggcgatcgc cgacgcggtc gacgagctct ggcgccaggg gccgggcgac
      841 gtgctcgtct tcctgcccgg cgagcgcgag atccgcgacg ccgccgaggc gctgcgcaag
      901 caccatccgc ccggcgtcga catcctgccc ctgttcgcgc gcctgtcgca ggccgagcag
      961 gaccgggtct tcgagaccgg cggcgcgccg cggatcgtgc tggcgaccaa cgtcgccgag
     1021 acctcgctga ccgtcccggg catccgctac gtcgtcgact cggggctggc gcgggtcaag
     1081 cgctacagct accgcacgaa ggtcgagcag ctgcagatcg agcccgtgag ccaggccgcg
     1141 tgcaaccagc gcgcgggccg ctgcggccgg gtttccaacg gcatctgcat ccgcctgttc
     1201 gacgaggccg acttcctggc ccgcccgcgc ttcagcgatc ccgagatcct gcgctcgtcg
     1261 ctcgcgggcg tgatcctgcg catgaagagc ctgcggctgg gcgcggtcga ggacttcccc
     1321 ttcctcgagg agccgccgcg caaagcgatc gccgacggct acgcgctgct gggcgaactc
     1381 aacgcggtcg acgacgccaa cgagctcacg ccggtcggcg tcgagctcgc gaagctgccg
     1441 ctggatccgc gcgtgggccg catgatcctc gaggcgcgcg accgccactg cctggccgag
     1501 gtgctggtga tcgcgtcggc gctgtcgggg caggacgtgc gcgaccggcc gctggagcag
     1561 cagcaggccg ccgacgagaa gcacaggaag ttcgacgacg agaagtcgga gttcatgggc
     1621 tacctcaagc tgtggaagtg gctcgagacg tcgcgcgggg ggcaggcgga ggcgataccc
     1681 gacgccgtat cc
//
LOCUS       DASVLN010000158         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_330215, whole genome shotgun sequence.
ACCESSION   DASVLN010000158 DASVLN010000000
VERSION     DASVLN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330215"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VF453_03610"
     CDS             <1..259
                     /locus_tag="VF453_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686765.1"
                     /translation="AWGVALPVAIVAAGLASPWGWLLALVYPLQMLRLGLRPAAAARE
                     AGAVWRRAFFLVAGRFPEAAGVLLFWRRKLRGRASTLIEYK"
     gene            316..1290
                     /locus_tag="VF453_03615"
     CDS             316..1290
                     /locus_tag="VF453_03615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7686766.1"
                     /translation="MTQTYALISPCRNEADYMRETLDTVVAQTATPALWVIVDDGSSD
                     DTPRILAEYAARHPWIRVVTRKDRGHRAVGPGVIDAFYAGYETIVPTDFDFVCKLDLD
                     LRLPPRYFEILMARMRAEPRLATCSGKAYVEERGGLIPERHGDETSLGMTKFYRVSAF
                     RDIGGFVREVMWDGIDCHECRRKGWIARSWDEPELRFVHLRPMGSSQQSIYVGRMRHG
                     FGQYFMGTGFLYMAASALLRTTQKPYVIGSAWMLWGWIKSALKGAPRYGDAEFRAFLR
                     RWQWRSLLVGKARATAEIERRSAARWQQGQAAPATPAPMPVSSTEGAA"
     gene            1287..2546
                     /locus_tag="VF453_03620"
     CDS             1287..2546
                     /locus_tag="VF453_03620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7686767.1"
                     /translation="MKVAYLVNQYPKVSHTFIRREIFALERQGLEVARFAIRGWDAEV
                     KGDEDLAERERTRHVLRGGIGSLLARAARRCIANPGRAWTALRAALAMGRQAERPWPY
                     HLVYFLEACTVADWLRDEGAAHLHAHFGTNSAEVARLAHLLSGVPYSFTVHGPEEFDK
                     PKFLHLADKIRDAAFVVAISSYGRSQLWRWLPAGDWAKVQVVHCALEKSFFAPSGRLT
                     VGTAPRLVNVGRLCAQKGQLLLVEAAARLAAAGAEFELVLCGDGEMRAPIEAAVARHG
                     LQRHVRITGWISSDTVREELLAARALVLPSFAEGLPVVVMEAMAVGRPVVTTRIAGIP
                     ELVRDGVDGWLVPPGSLEDLVQAMGACLAASPEALARMGASAHARVQERHDADVEAAK
                     LKALFTGSVAPDASRAAAAAPAGPRTA"
BASE COUNT          328 a          934 c          937 g          354 t
ORIGIN      
        1 cgcgtggggc gtcgcgctgc ccgtggccat cgtcgccgcg gggctcgcct cgccgtgggg
       61 ctggctgctg gcgctggtct acccgctgca gatgctgcgg ctgggcctgc gtccggccgc
      121 ggccgcgcgc gaggcgggag cggtctggcg gcgcgcgttc ttcctcgtcg ccgggcgctt
      181 cccggaggcg gcaggcgtct tgcttttctg gcgcaggaag ctgcgcggcc gcgccagcac
      241 cctgatcgag tacaagtagg cgccaaggcg agtcgtttgc ctcgcgcgcg cctgccacaa
      301 gccctccatg cagccatgac ccagacctat gccctcatct cgccgtgccg caacgaggcg
      361 gactacatgc gcgagaccct cgacacggtg gtcgcacaga ccgccacgcc ggccctgtgg
      421 gtgatcgtgg acgacggctc ctcggacgac acgccgagga tcctcgccga atacgcggcg
      481 cgccacccct ggatccgcgt cgtcacgcgc aaggaccgcg gccaccgcgc cgtcggcccg
      541 ggcgtaatcg acgccttcta cgccggctac gagacgatcg tgccgacgga cttcgacttc
      601 gtctgcaagc tcgatctcga cctgcgcctg ccgccgcgct acttcgagat cctgatggcg
      661 cgcatgcgcg cggagccgcg cctggcgacg tgcagcggca aggcctacgt cgaggagcgc
      721 ggcggcctga tccccgagcg ccacggcgac gagacctcgc tgggcatgac caagttctac
      781 cgcgtcagcg cgttccgcga catcggcggc ttcgtgcgcg aggtcatgtg ggacggcatc
      841 gactgccacg aatgccgccg caagggctgg atcgcgcgca gctgggacga gccggagctg
      901 cgtttcgtgc accttcggcc gatgggctcg agccagcaga gcatctacgt cggccgcatg
      961 cgccacggct tcggccagta cttcatgggc acgggcttcc tgtacatggc cgccagcgcg
     1021 ctgctgcgca cgacgcagaa gccgtacgtg atcggcagcg cgtggatgct gtggggctgg
     1081 atcaagagcg cgctcaaggg cgcgccgcgc tacggcgacg ccgagttccg cgccttcctg
     1141 cgccgctggc agtggcgctc gctgctggtg ggcaaggcgc gcgcgaccgc ggagatcgag
     1201 cggcgcagcg ccgcgcgctg gcagcagggc caggccgcgc cggcgacgcc cgcgccgatg
     1261 ccggtctcgt ccacggaggg cgcggcgtga aggtcgccta cctcgtcaac cagtacccga
     1321 aggtcagcca caccttcatc cggcgcgaga tcttcgcgct cgagcgccag ggcctggagg
     1381 tcgcgcgctt cgcgatccgc ggctgggacg ccgaggtcaa gggcgacgag gacctcgccg
     1441 agcgcgagcg cacccggcac gtgctgcgcg gcggcatcgg cagcctgctc gcgcgcgcgg
     1501 ccaggcgctg catcgccaac ccgggccgcg cctggacggc gctgcgcgcg gcgctggcga
     1561 tgggccggca ggccgagcgt ccgtggccgt accacctcgt ctacttcctc gaggcgtgca
     1621 cggtcgccga ctggctgcgc gacgaaggcg ccgcccacct gcacgcgcac ttcggcacca
     1681 actccgcgga agtcgcgcgc cttgcgcacc tgctctcggg cgtgccgtac agcttcacgg
     1741 tgcacggccc ggaggagttc gacaagccga agttcctgca cctggcggac aagatccgcg
     1801 acgccgcgtt cgtggtggcg atcagcagct acggccgcag ccagctctgg cgctggctgc
     1861 cggcgggcga ctgggcgaag gtgcaggtgg tgcactgcgc gctcgagaag tccttcttcg
     1921 cgccgtccgg gcgcctgacc gtcggcaccg cgccgcgcct ggtcaacgtc ggccggctgt
     1981 gcgcgcagaa gggccagctg ctgctggtcg aggccgccgc gcgcctggcc gccgcgggtg
     2041 ccgagttcga gctggtgctg tgcggcgacg gcgagatgcg cgccccgatc gaggccgcgg
     2101 tcgcccgcca cggcctgcaa cgccacgtcc gcatcacggg ctggatctcg agcgacacgg
     2161 tgcgcgagga gctgctcgcc gcccgcgcgc tggtgctgcc cagcttcgcg gagggcctgc
     2221 ccgtcgtcgt gatggaggcg atggccgtcg gccgcccggt cgtgacgacg cgcatcgccg
     2281 gcattccgga gctcgttcgc gacggtgtcg acgggtggct ggtgccgccg ggcagcctcg
     2341 aggatctcgt gcaggcgatg ggtgcctgcc tggccgcgtc gcccgaggcg ctcgcgcgca
     2401 tgggcgcgag cgcccatgcg cgcgtgcagg agcgccacga cgcggacgtc gaggcagcca
     2461 agctgaaggc gctgttcacc ggcagcgtcg ccccggacgc gtcacgcgcc gcggcggcgg
     2521 cacccgccgg cccccgcacc gcctgagccg gcc
//
LOCUS       DASVLN010000159         4417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_8529, whole genome shotgun sequence.
ACCESSION   DASVLN010000159 DASVLN010000000
VERSION     DASVLN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8529"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="VF453_03625"
     CDS             <1..1423
                     /locus_tag="VF453_03625"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX7686768.1"
                     /translation="GTGNAAAAVAAATGQQVNPTPQTPPLPALPPGTAPNYRAIVQEF
                     GPCVVHVSVEGTHKPTAEEAEENGFGGGPVFRFPGIPGFPGFGIQRGGQMPEQPFKGL
                     GSGFIIGSDGLILTNAHVVKEAKDVTVKLQDRREYVAKVLGVDTATDIAVLKIDAKGL
                     PVVRFGQPSKLMVGDYVLAIGAPYGLDQTATSGIISAKGRSLPSENGENFVPFLQTDA
                     AVNPGNSGGPLFDAAGNVIGINSQIYSRTGGFQGVSFAIPIDVALAIKDQIIATGHAQ
                     HARLGVSIQPLNQDLAESFGLDRPDGALVSTVAPNSGAAKAGLKSGDVITKINGERIE
                     EPGQLSSRIGMSKPGDKVTLEVWRDHKTETVTATLGALNDKTVTAQGGDTIDGAKLGL
                     ALRPLNRDERGEVGQSGLVVESADGAAAKAGVEQGDVVLAVNGKPVASVDDVKAILAA
                     KPKRVALLIWRDGERIFFPVNLG"
     gene            complement(1597..1797)
                     /locus_tag="VF453_03630"
     CDS             complement(1597..1797)
                     /locus_tag="VF453_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686769.1"
                     /translation="MKNVLIASVLVAFAAAASAQGAPASAAQTGNASPDVAASAVAKH
                     KAKHAAKKKAKKAAKAASAASM"
     gene            2240..4345
                     /locus_tag="VF453_03635"
     CDS             2240..4345
                     /locus_tag="VF453_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7686770.1"
                     /translation="MAATPEYRLLNADTAAAAAPLAGAGLNPAQMEAVMHLDGPCLVL
                     AGAGSGKTRVITYKIVRLLAAGLKPGQIAAITFTNKAAQEMRERARALVGGKAAKDLA
                     ISTFHSLGVRMLREDGQRLGLKEQFSILDSDDVLGVLKDAGSATDNKLARQWQWTISL
                     WKNMGYTSDDALRVAKDAEEQVAARVMKLYEERLAAYSAVDFDDLIGLPLKLLRRDEQ
                     ARAKWQALFRHVLVDEYQDTNAVQYELLKQLVGEPDRARFTAVGDDDQSIYGWRGATI
                     ENLRRLPEEYPSLKVIPLEQNYRSTTSILRAANEVIAANPKLFPKKLWSEHGDGEPVR
                     VVECDGEEHEAERAVARIQSLRGNDGRVKFSDCAILYRANHQSRVFEQKLRAAQIPYK
                     VSGGQSFFDRAEIKDLCAWLRLIVNEDDDPAFLRAVTTPKRGIGHATLGALSEFAGKW
                     KVSMFEALFSESLGVALKSKAIDGLHEFGRYVNDFQHRAKTTVGAEAAKPMLMEWLKD
                     IDYEKHLYDNEDSEKAAQSRWQNVLDFVDWVARRCGGEITQEGGTFESERKSVLDIAQ
                     SISVILSLAERGDEQDVVTLSTLHASKGLEWPHVVLVGINEGLLPFKSDGEDMTPERL
                     QEERRLMYVGITRARETLSVSTLRRRKKGRDTVAGVPSRFIAEMKLNEAKAQENPMER
                     LKRLRAELAAKNAGAAPAA"
BASE COUNT          715 a         1583 c         1551 g          568 t
ORIGIN      
        1 cggcacgggc aacgccgccg cggccgttgc cgccgccact ggccagcagg tcaacccgac
       61 gccgcagacg ccgccgctgc ccgcgctgcc gccgggcacg gccccgaact accgcgccat
      121 cgtgcaggag ttcggcccct gcgtggtgca cgtcagcgtc gaaggcacgc acaagccgac
      181 cgccgaggag gccgaggaga acggcttcgg cggcgggccg gtcttccgct tcccgggcat
      241 ccccggcttc ccgggcttcg gcatccagcg cggcggccag atgcccgagc agcccttcaa
      301 gggcctcggc tcgggcttca tcatcggctc ggacggcctg atcctcacca acgcgcacgt
      361 cgtcaaggaa gccaaggacg tcaccgtcaa gctccaggat cgccgcgagt acgtcgccaa
      421 ggtgctgggg gtggataccg ccaccgacat cgccgtcctc aagatcgacg ccaagggcct
      481 gcccgtggtg cgcttcggcc agccgtccaa gctgatggtg ggcgactacg tgctcgcgat
      541 cggcgcgccc tacggcctcg accagacggc cacctccggc atcatcagcg ccaagggccg
      601 ctcgctgccg agcgagaacg gcgagaactt cgtgccgttc ctgcagacgg acgcggcggt
      661 caaccccggc aactcgggcg gcccgctgtt cgacgccgcg ggcaacgtga tcggcatcaa
      721 ctcgcagatc tacagccgca ccggcggctt ccagggcgtg agcttcgcga tcccgatcga
      781 cgtggcgctg gcgatcaagg accagatcat cgccaccggc cacgcgcagc acgcgcgcct
      841 gggcgtcagc atccagccgc tgaaccagga cctcgccgag agcttcggcc tcgaccgccc
      901 ggacggcgcg ctggtctcca ccgtcgcgcc caacagcggc gcggccaagg ccggcctgaa
      961 gtcgggcgac gtgatcacca agatcaacgg cgagcgcatc gaggagccgg gccagctgtc
     1021 ctcgcgcatc ggcatgtcca agccgggcga caaggtcacg ctggaggtct ggcgcgacca
     1081 caagaccgag accgtgacgg cgacgctggg cgcgctcaac gacaagaccg tcacggcgca
     1141 gggcggcgac accatcgacg gcgccaagct gggcctggcg ctgcgtccgc tcaaccgcga
     1201 cgagcgcggg gaggtgggcc agagcggcct cgtggtcgag agcgccgacg gcgccgccgc
     1261 caaggcgggc gtggagcagg gcgacgtcgt gctggccgtc aacggcaagc cggtggcctc
     1321 ggtcgacgat gtcaaggcga tcctggcggc caagcccaag cgcgtcgcgc tgctgatctg
     1381 gcgcgacggc gagcggatct tcttcccggt caacctgggc tgatccccgt cggggcgggc
     1441 cgtcgcccgc ccgatgggcc caagggcgtc ccggcggacg cccttttgct tcgggcgccc
     1501 gatcgccggg cacaaaaaag ccgaccgggc gcgagccggg tcggcttttc ctcgcgcgcg
     1561 gccggcgccc tgagcaccgg ccgcgcacgc gtcgacttac atcgaggcgg cggaggcggc
     1621 cttcgcagcc ttcttcgcct tcttcttggc ggcgtgcttg gccttgtgct tggcgacggc
     1681 cgaggcggcg acgtccggcg aggcgttgcc ggtctgggcg gcggaagccg gggcgccctg
     1741 ggccgaggcg gcggcggcga aggcgacgag gacggaggcg atgaggacgt tcttcatggc
     1801 gaacctttcg ggtgggaaat ggaatgggga cgtccgctta acgagcgcag gcgacgaacg
     1861 gtttacgcgc gggcgaagat ttctcgcgcc ggccgaatgt aaagatggcg gctgcgcggc
     1921 cggcgaaggc tgctgcgccc gccgcgaagc gtcgacgcga gcgcgcaggt cggccagcgc
     1981 ggaaaagccg cccgaaaccg cgccgtccgt cacgggcgcc gccgggggcg ttcgctatca
     2041 tctcgactcg cgcggcgccg ccgtcgtcgc gtccaccggc gcgcgcccgc ggccctcgtt
     2101 gcaatcgctt accgcccgcc gcggcgccga cccgtgcagg gggctcgcga cgtgcggttc
     2161 gaacccctcc gtttccgctc cctttccact ccggttccat cgccggccgc gcctgccgcc
     2221 cgccgcgtcc ttccgcccca tggccgccac ccccgaatac cgactcctga acgccgacac
     2281 cgccgcggcg gccgccccgc tggccggggc cggcctcaat cccgcgcaga tggaggccgt
     2341 gatgcacctg gacggcccgt gcctcgtgct cgcgggcgcc ggctcgggca agacgcgcgt
     2401 gatcacctac aagatcgtcc gcctgcttgc cgccgggttg aagccgggcc agatcgcggc
     2461 catcaccttc accaacaagg cggcgcagga gatgcgcgag cgcgcccgtg cgctggtcgg
     2521 cggcaaggcg gccaaggacc tcgcgatcag cacgttccac tcgctgggcg tgcgcatgct
     2581 gcgcgaggac ggccagcgcc tcggcctgaa ggagcagttc tccatcctgg acagcgacga
     2641 cgtgctcggc gtgctcaagg acgccggcag cgcgaccgac aacaagctcg cccgccagtg
     2701 gcaatggacg atcagcctgt ggaagaacat gggctacacg agcgacgacg ccctgcgcgt
     2761 ggccaaggac gccgaggaac aggtcgccgc gcgcgtgatg aagctctacg aggagcgcct
     2821 ggccgcgtac tcggcggtcg acttcgacga cctgatcggc ctgccgctga agctgctgcg
     2881 ccgcgacgag caggcgcggg ccaagtggca ggcgctgttc cgccacgtgc tggtcgacga
     2941 gtaccaggac accaacgccg tccagtacga gctcctgaaa cagctcgtcg gcgagcccga
     3001 ccgcgcgcgc ttcaccgccg tgggcgacga cgaccagagc atctacggct ggcgcggcgc
     3061 gacgatcgag aacttgcgcc gcctgcccga ggaatacccg tcgctcaagg tcatcccgct
     3121 cgagcagaac taccgctcga ccacctccat cctgcgcgcc gccaacgagg tgatcgcggc
     3181 caaccccaag ctgttcccga agaagttgtg gagcgagcac ggcgacggcg agccggtgcg
     3241 cgtcgtcgag tgcgacggcg aggagcacga ggccgagcgc gccgtcgccc gcatccagtc
     3301 cctgcgcggc aacgacgggc gcgtgaagtt ctccgactgc gcgatcctgt accgcgccaa
     3361 ccaccagtcg cgcgtgttcg agcagaagct gcgtgccgcg cagatcccgt acaaggtctc
     3421 gggcggccag agcttcttcg accgcgccga gatcaaggac ctctgcgcct ggctgcgcct
     3481 gatcgtcaac gaggacgacg acccggcctt cctgcgcgcc gtcacgacgc cgaagcgcgg
     3541 catcggccac gcgacgctgg gcgcgctcag cgagttcgcc ggcaagtgga aggtcagcat
     3601 gttcgaggcg ctgttctcgg agtcgctggg cgtcgccctg aagtcgaagg cgatcgacgg
     3661 cctgcacgag ttcggccgct acgtgaacga cttccagcac cgcgcgaaga ccaccgtcgg
     3721 cgccgaggcc gccaagccga tgctgatgga gtggctcaag gacatcgact acgagaagca
     3781 cctctacgac aacgaggact ccgagaaggc cgcgcagtcg cgctggcaga acgtgctgga
     3841 cttcgtcgac tgggtcgcgc ggcgctgcgg cggcgagatc acgcaggagg gcggcacctt
     3901 cgagagcgag cgcaagagcg tgctggacat cgcgcagtcg atcagcgtca tcctgagcct
     3961 ggccgagcgc ggtgacgagc aggacgtcgt cacgctgtcg acgctgcacg cgtccaaggg
     4021 cctcgagtgg ccgcacgtgg tgctggtggg catcaacgag ggcctgctgc ccttcaagtc
     4081 cgacggcgag gacatgacgc ccgagcgcct gcaggaggag cgtcgcctga tgtacgtggg
     4141 catcacgcgc gcgcgcgaga cgctgtcggt gagcaccctg cgccgacgca agaagggccg
     4201 cgacaccgtg gccggcgtgc cgagccgctt catcgcggag atgaagctca acgaggcgaa
     4261 ggcgcaggag aacccgatgg aacgcctcaa gcgcctgcgc gccgagctgg ccgcgaagaa
     4321 cgccggcgcc gcgccggcgg cctgaacggc ccgggttcga gcgcgcactc tagcccgcgc
     4381 gcgggcaatc cctgagcggc gtcccgcacg cccgcgg
//
LOCUS       DASVLN010000160         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_330420, whole genome shotgun sequence.
ACCESSION   DASVLN010000160 DASVLN010000000
VERSION     DASVLN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330420"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VF453_03640"
     CDS             <1..438
                     /locus_tag="VF453_03640"
                     /inference="COORDINATES: protein motif:HMM:NF026049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX7686771.1"
                     /translation="AYSKDMQDDKPPVFEAAGLLALSLAAMAGMVADATFDTARMRQA
                     AELGYATATDLADWLARTAGVPFREAHHITGAAVKLAEQKGVALDAVPLAELQEIDPR
                     IGKGALAALSVDASVAARASHGGTAPAEVEKRVAEARAALGIA"
     gene            435..674
                     /locus_tag="VF453_03645"
     CDS             435..674
                     /locus_tag="VF453_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686772.1"
                     /translation="MIRLIIIAGSALLLVACAQTAELKPPPGKTLPVAPYGREDKPSA
                     EELLQPSAQAVPERSVELQTRSQPREDDPFDLPPQ"
     gene            676..1935
                     /gene="lysA"
                     /locus_tag="VF453_03650"
     CDS             676..1935
                     /gene="lysA"
                     /locus_tag="VF453_03650"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7686773.1"
                     /translation="MDHFQLRNGELYAEDVPLARIADEVGTPVYVYSRATLVRHARVF
                     REALAGVPGAHLAFAVKSNPNLAVLKVLQKEGYGADVVSGGELERALRAGMAPADIVF
                     SGVGKNARELERGLDAGIGQFNIESEEEGRELAEIAAARGIAARCALRVNPDVDAGTH
                     DKISTGKKENKFGVAIDEAPGIYARLAGLSGLDMRGVAVHIGSQLADLAPLEKAFARL
                     GQLIGELRAAGLKVTHADLGGGLGVPYKAGEVLPSPAEYGAMVARVTKGWGVALMFEP
                     GRVIAGNAGILLTRVVRVKRSGNRTPFVIVDAAMNDLARPALYGAWHDFDAVRPQGQR
                     MVANVVGPICETGDTFATEREIDALEPGELAVFRTAGAYGATMASSYNSRGFVPEVLV
                     DGDRFAVVAERIAPAAIADAERVPDWL"
     gene            1945..>2445
                     /locus_tag="VF453_03655"
     CDS             1945..>2445
                     /locus_tag="VF453_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEX7686774.1"
                     /translation="MRSLPLFHRIAGRKVIVLGEGPLAEAKARLVERAGGIVASEGDP
                     DARLAFVALDEPEAATARLKARGLLVNVADRPELCDFTVPSLLDRDPVLIAVGTSGAS
                     AGLAKQLRLRLEALLPQSLGALAEALQAARGKLRERFPGADDRRRALDAALAAGGPLD
                     PLGPAHA"
BASE COUNT          360 a          833 c          917 g          335 t
ORIGIN      
        1 gcctattcga aggacatgca ggacgacaag ccgccggtgt tcgaggcggc ggggctgctg
       61 gcgctgtcgc tcgccgcaat ggcgggaatg gtcgcggacg cgacgttcga caccgcaagg
      121 atgcgccagg cggccgagct cggctatgcg actgcgaccg atctcgccga ctggctggcg
      181 cgcaccgccg gggtgccgtt ccgcgaggcg caccacatca ccggcgcggc ggtgaagctg
      241 gccgaacaga agggcgtcgc gctcgatgcg gtgccgctcg ccgaactcca ggagatcgat
      301 ccacggatcg gcaaaggcgc gctcgccgcg ctgtcggtcg atgcgtcggt cgccgcgcgc
      361 gcgagccatg gcggaaccgc gccggccgag gtagagaagc gcgtcgccga agcgcgcgcg
      421 gcgctgggaa tcgcatgata cgtctgatca tcatcgccgg atcggctctg ctgctcgtcg
      481 cttgcgcgca gaccgcggag ctcaagccgc ccccgggcaa gaccctgccg gtcgcgccat
      541 acggccgcga ggacaagccg agcgccgaag aactgctgca gccgagcgcg caggcggtgc
      601 ccgagcgcag cgtcgaactg cagacccgct cgcagccgcg cgaagacgat ccgttcgacc
      661 tgccgccgca ataagatgga ccactttcag ctcaggaacg gcgagcttta cgccgaggac
      721 gtgccgctgg cccggatcgc cgacgaagtc ggcacgccgg tctacgtcta ttcgcgcgcc
      781 accttggtgc gccacgcgcg ggtgttccgc gaggcgctgg cgggtgtgcc aggcgcgcac
      841 ctcgcattcg cggtcaagtc caatcccaac ctcgcggtgc tcaaggtgct ccagaaggag
      901 ggctacggcg ccgacgtggt ttcgggcggc gagctcgaac gggcgctgcg cgcgggcatg
      961 gcgccggcgg acatcgtgtt ctcgggcgtc ggcaagaacg cgcgcgagtt ggagcgcggc
     1021 ctcgatgccg gaatcggcca gttcaacatc gagtcggagg aagagggccg cgagctggcc
     1081 gagatcgcgg ccgcgcgcgg catcgcggcg cgctgcgcgc tgcgcgtgaa tcccgacgtc
     1141 gatgccggca cccacgacaa gatctcgacc ggcaagaaag agaacaagtt cggcgtcgcg
     1201 atcgacgagg cgccgggtat ctatgcgcga ctggccgggc tttccgggct cgatatgcgc
     1261 ggcgtcgcgg tgcacatcgg cagccagctt gccgatctcg cgccgctgga gaaggccttc
     1321 gcgcggctgg ggcaactgat cggcgagctc cgcgcggcgg ggctcaaggt gacccacgcc
     1381 gatctcggcg gcggcctcgg cgtgccctac aaggcgggcg aggtgctgcc gagcccggcc
     1441 gagtacggcg caatggtcgc acgtgtgacc aaagggtggg gcgttgccct gatgttcgag
     1501 cccgggcggg tgatcgcggg caacgccggc atcctgctga cccgggtggt gcgggtgaag
     1561 cgcagcggca accgcacgcc gttcgtgatc gtcgacgcgg cgatgaacga cctcgcccgg
     1621 ccggcgctct acggcgcgtg gcacgatttc gacgcggtgc ggccccaggg ccagcggatg
     1681 gtcgccaacg tggtggggcc gatctgcgag accggcgaca cgttcgcgac cgagcgcgag
     1741 atcgatgcgc tcgagccggg cgagctggcg gtgttccgca ccgccggcgc ctatggcgcg
     1801 acgatggcct cgagctacaa cagccgcggg ttcgtgcccg aagtgctggt cgacggggat
     1861 cgcttcgcgg tggttgccga acggatcgcg ccggccgcga tcgccgacgc cgaacgggtg
     1921 ccggattggc tctgagcaac tgagatgcgc tcgctgccgc tcttccatcg catcgccggc
     1981 cgcaaggtga tcgtgctggg agaagggcca cttgccgagg ccaaggcgcg gctggtcgag
     2041 cgcgccgggg gcatcgtcgc gagcgagggc gatcccgacg cccgcctggc gttcgtcgcg
     2101 ctcgacgagc ccgaagcggc gaccgcccgg ctaaaggcgc gtgggctgct ggtcaacgtc
     2161 gccgaccggc ccgagctgtg cgacttcacg gtgccgagcc tgctggaccg cgatccggtg
     2221 ctgatcgcgg tcggcacctc gggcgcatcg gccgggctcg ccaagcagtt gcggctgcgg
     2281 ctcgaggcat tgctgccgca gtcgctcggg gcgctcgccg aggcgcttca ggccgcgcgc
     2341 gggaaactgc gcgagcgctt tcccggcgcc gacgaccgcc gccgcgcgct cgatgccgcg
     2401 ctcgccgcgg gcggcccgct cgatccgctg ggccctgccc acgcg
//
LOCUS       DASVLN010000161        15553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_169965, whole genome shotgun sequence.
ACCESSION   DASVLN010000161 DASVLN010000000
VERSION     DASVLN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15553
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169965"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1238)
                     /locus_tag="VF453_03660"
     CDS             complement(30..1238)
                     /locus_tag="VF453_03660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7686775.1"
                     /translation="MTREAAAPSAGPTLVVWPAVPAWRIDGELWLDRKFKDGQDEFAA
                     RWPGRIKAVMDVVDLPAMPAFGACRWTGAGEAWSLELIPRGEAFGRHHIAGADVLLAS
                     ADSPRKLVAPDLCRGVGTACVMAIEYTLRTRLDMLRQAPMTPLKRLKTLAWHLRNEPR
                     IRRALRECDGVQANGQAAFDAYTRGRPHGLLYWDTRLAAADVVGEAALEARLATLAAP
                     RPLRLAFSGRLIAAKGADALVPLAAALQRRGVPVHFDVWGAGELEAGMRADIARLGLG
                     SSMRVNGPVDFAGVLMPTLQRDVDLFVCCHRQGDPSCTYAETLGCGVPIAGFANESLS
                     ALVASHGVGWVVPMGAIERLADVVERLHGARGELADAARRARGFGVRNDFETVVAQRT
                     AHCLAVAQAR"
     gene            complement(1235..2287)
                     /locus_tag="VF453_03665"
     CDS             complement(1235..2287)
                     /locus_tag="VF453_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX7686776.1"
                     /translation="MSKLGQIGDILRYLAQDSHSIHYTGWVGYRNLGDEILLEAAQEC
                     FAPARIVWIREFHNARARALVARKRHELAMLGGGTQIGERSPIDRFRRAIAASKAAFV
                     FGAGVTPAVDGPIPSWLEQWGEVLRPLAYVGVRGPESAATLRRAGVDAQVLGDPVAWF
                     TRPVDFWQPTEGLIGVNVGHSHGHMYGQESTIQTQLAQLVRELGARGHRFEFFCVWPE
                     DLETTRRIAAEAGVKDPVIHEIYEGARRFQEAVRRMQVFVGIKLHAVALAMCSSVPSL
                     MIEYRPKCREFATTMGVQEFCVRSDALDVGQMLALVDDLKARGRETATVMNAHGNAIR
                     GRLEALSKSLLSSHGW"
     gene            2590..3357
                     /locus_tag="VF453_03670"
     CDS             2590..3357
                     /locus_tag="VF453_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEX7686777.1"
                     /translation="MLNVWADDIPMDDLLARLATEGGIVFTVNPDHLYHLQHNPAFLH
                     AYRCADVITVDSHYIRIVLRLLGRPVRHRLPGSDIVPALCRRQAQDPATRIFLLGARP
                     GVAQRAREAINARAGRELVVGAHGPSMNFVNDPAEIDATIDMINASGATVLLVGLGAP
                     KQEIWIASVRHRLPHVRAMMGVGATIDYEAGEVRRAPQLMRSLGLEWFYRVATEPGRY
                     LMRYVRSSRFIWWMLMDRLGRYKDPFAGPASASVQSR"
     gene            3426..4676
                     /locus_tag="VF453_03675"
     CDS             3426..4676
                     /locus_tag="VF453_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686778.1"
                     /translation="MQLKPAFSLTPLVLCLAAGSAHADLSPYSFGASETIQHQSNIDH
                     GPGNGPGDWLSTTELRGGLDQPLGRDELKANVAIDFNRYKNFHRRNATGYLASTEFDW
                     SLPADLSGSFGAETRRRQYVYGLNGEALGGSGGSDQRNLETDNHFYARGQIGGESRWT
                     LYGGLDATNRRYSLASFSGNDERQWSGNVGTRYATSPDLSFSLQASYSHGEYPNFQQQ
                     AGGPVVDDTFSMRSYDLVTNYVASGNSKLDASVGFTTQTSDIQSARHFVNGSLNWTWT
                     PPSHFNVRVGLARDSDADTDAVNVSFNPNGINGRSINNVAHADIGYELTAKVSLDLLA
                     QYTQRKYSNAVLPVLDAQGKPQVANGTNNTRRVYLSAHYQPTRTTDLSCGAGREIRRS
                     HVSGDLTPGYTDNTVQCVAAIDFK"
     gene            4732..6012
                     /locus_tag="VF453_03680"
     CDS             4732..6012
                     /locus_tag="VF453_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7686779.1"
                     /translation="MNTVAVVGLGYVGLPLVIEFGKLGRTIGFDIAADKVARCRAGTD
                     PSREIPDAEMALATHAEYTSDPADLRDADVVIVAVPTPVDHAHIPDFGPLIGASRSIG
                     PHLKPGATVVYESTVYPGATEEVCIPELERSSGKRWKRDFFVGYSPERINPGDREHTL
                     TRVVKVVAGDMPETLERVAALYERIVQPGVHRCSSIKAAEACKVIENTQRDLNIALMN
                     ELAIIFDKVGIDTSEVLEAAGTKWNFLKFKPGLVGGHCIGVDPYYLTHKAETLGYHPQ
                     VILAGRRINDGMGKFIAEQTVKQMIAAGSSVKGARVNVLGLTFKEDCADLRNSKVVDI
                     VAELKSYGVEVFVHDPQADAAEARHEYGVELVAWDDLPRADAVVAAVAHREFKALGVD
                     RIAEHLVGGGVFIDVKAAFPEGELLARGLRVWRL"
     gene            6029..7051
                     /locus_tag="VF453_03685"
     CDS             6029..7051
                     /locus_tag="VF453_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEX7686780.1"
                     /translation="MKVLVTGAAGFIGMHVAQRLLARGDEVVGIDSLNSYYDPALKAA
                     RLAELARDPRAAAFRFERLDVADGAALQALFARERFDRVVHLAAQAGVRYSIENPQAY
                     GEANLAGFLNVLEACRRHPVEHLVYASSSSVYGGNAKMPFSEADSVDHPVSLYAATKK
                     ANELMAHTYSHLYALPTTGLRFFTVYGPWGRPDMAYFSFTRDILAGRPIAVFNEGRML
                     RDFTYINDIVDGVVAVLDKPAAPDPGFEPLAPNPGTSRAPYRVFNIGNQDPVALGEFI
                     ATLERALGVEAIRDYRPMQPGDVVATHADVSALKAWTGVSPRTPLADGLARFVEWYRR
                     YFADRA"
     gene            7103..8161
                     /gene="rfbB"
                     /locus_tag="VF453_03690"
     CDS             7103..8161
                     /gene="rfbB"
                     /locus_tag="VF453_03690"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX7686781.1"
                     /translation="MILVTGGAGFIGSNFVLDWLENHDEPVLTLDALTYAGNLANLES
                     LQGDPRHVFQHGDIGDRALVDALLARHQPRAIVNFAAESHVDRSILGPGAFLKTNIEG
                     TFTLLEAARTYHATLEGEDRAAFRFLHVSTDEVYGSLTPDAAPFTENHPYEPNSPYSA
                     SKAASDHLVRAWHHTYGLPTLTTNCSNNYGPLHFPEKLIPLVIVNALAGKPLPVYGDG
                     LNVRDWLYVRDHCAAIREVLARGRVGETYNVGGLNEKTNIDIVRGICRLLDELRPDPA
                     GPRERLITFVKDRPGHDRRYAIDATKLCTELGWRPQETFETGLRKTVQWYLANAEWVQ
                     GVQSGAYRDWVSTQYGKH"
     gene            8175..9071
                     /gene="rfbD"
                     /locus_tag="VF453_03695"
     CDS             8175..9071
                     /gene="rfbD"
                     /locus_tag="VF453_03695"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX7686782.1"
                     /translation="MKMLLFGAGGQVGWELQRSLQPLGELVALDFDSRDLPADFSRPE
                     SLAATVEAVRPDVIVNAAAHTAVDKAESEPALAQAINADAPGAIARAAAASGAWLVHY
                     GTDYVFDGSGDQPRDETAPTAPLSVYGRTKLAGEEQIRASGCRHLILRTSWVYAARGG
                     NFARTMLRLAGTKTELSVIADQVGAPTGADLLADLSAHMIRAARATPELAGTYHAVAG
                     GFTSWHGYATLVIETARGRGMPLTVPAGAIRPIPTSAYPTPAPRPLNSRLDTRKLRER
                     FGLSLPPWEAGVRRMLDEILPA"
     gene            9103..9984
                     /gene="rfbA"
                     /locus_tag="VF453_03700"
     CDS             9103..9984
                     /gene="rfbA"
                     /locus_tag="VF453_03700"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX7686783.1"
                     /translation="MNRKGIILAGGSGTRLHPATLAISKQLIPVYDKPMIYYPLSVLL
                     LAGIREILVISTPQDTPRFEQLLGDGRRWGLELRYVVQPSPDGLAQAFLIGEDFLGGA
                     PSALVLGDNIFYGHDLGGRLEAANARAECATVFAYPVHDPERYGVVEFDAGGRAISLE
                     EKPARPKSRYAVTGLYFYDEDVVAHAKALAPSPRGELEITDLNRRYLEAGKLDVQILG
                     RGDAWLDTGTHDSLLEASQFVQTLEKRQGLKACCPEEICWRQKWIDDAQLAALAAPLA
                     KSGYGQYLQRLLSERVF"
     gene            9984..10526
                     /gene="rfbC"
                     /locus_tag="VF453_03705"
     CDS             9984..10526
                     /gene="rfbC"
                     /locus_tag="VF453_03705"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX7686784.1"
                     /translation="MNVETTAIEGVLILQPKVFGDARGFFLESFNQKVFDEAVGRHVD
                     FVQDNHSRSARGVLRGLHFQRGEKAQGKLVRVVAGAVFDVAVDIRRDSPSFGRWVGVE
                     LSADNHRQLWVPPGLAHGFLVLSETADFLYKTTEFYSPQHEGAVRWDDPDLAIAWPDV
                     GAAPTLSAKDAAAGTLRDLP"
     gene            10745..11839
                     /locus_tag="VF453_03710"
     CDS             10745..11839
                     /locus_tag="VF453_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX7686785.1"
                     /translation="MILAAGQGTRVRPLTKNLPKPMIPILGKPVLEYLIEHLARYGVN
                     EIMINVAYTHWKIENYFGNGSRWGVEIGYSFEGTRDHGDIVPKPMGSAGGMRKIQDHS
                     GFFDETTIVLCGDAVIDLDIGAALHEHKTKQALASVVTLEVPEEEVKNYGVVVTDKDG
                     RITEFQEKPSPAEAKSRLASTGIYIFEPQVLDLIPSGQTFDIGSQLFPLLAERGLPFF
                     AQSRWFNWIDIGRVTDYWAVCQRVLRGEVAQMDMPGREVRPGVWVGLNTSIDFDKVDI
                     TGPVYIGSSSVIEPGAKVVGPAWLGNGCILRQGSKLVRSILFDYVRLSQGETLEDMIV
                     SPYYCVDRHGNTTYPGDESKPLRWGDARAS"
     gene            11914..13359
                     /locus_tag="VF453_03715"
     CDS             11914..13359
                     /locus_tag="VF453_03715"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX7686786.1"
                     /translation="MSTAIPIQPVIMAGGSGTRLWPLSRAGFPKQFLALAGDRSLFQE
                     AVARLAGLAAGDIAVRAPLVVGNAEHRFLALEQLREQGVEPASVILEPVGRNTAPALT
                     LAALAATEGDADPVLVVSPADQTVTDVPAFTAALQQAVRGAAGGAIVILGITPDRPET
                     GYGYIRSVAGDDGLARVAQFVEKPDAPTAARYLDEGGYTWNSGMFVLRASTWLKALEA
                     FRPDILAATRAAWQGREPDARFVRPTREAFLAVPAESVDYAVMERCPGSAFDIRMVPL
                     AAGWNDLGAWDAVWQVASKDGAGNVAVGDVMFEDSRNTLVHASGRLVTTVGLDDVVIV
                     ETADAVLVADRARSQDVKKLVGRLDSGRRTEHALHRKVHRPWGWYDSIDAGPRFQVKR
                     IMVNPGASLSLQMHHHRAEHWIVVQGTAEVTCGARKVILTENQSTYIPLGETHRLANP
                     GKLPLEIIEVQSGSYLGEDDIVRFEDTYGRN"
     gene            13465..14859
                     /locus_tag="VF453_03720"
     CDS             13465..14859
                     /locus_tag="VF453_03720"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEX7686787.1"
                     /translation="MFEERSVRKQFLSAPPSIGSVIASLLEPLVAAGALAVFHAIYGY
                     RMDGAAMALAILLLVLMFPGVNRFGRTGVGVGIDIILSWVWVLTVLALLGYATDSLNA
                     FDPRMLTGWAIATPLVQWALVAIGTAIQRHLASLPDARRPAVIIGAGRMGMRVAQMLR
                     QRRSFGHDLLGFFDDRSADRVNLPPDALLLGALKGLPEYIEVHGVKDVFITLPLTSQP
                     RIQSLLESLQNTTASIHFVPDIFGVSVIQGRLEDMGGVPVVGLMVAPFTGINGFVKRA
                     SDIVLSGLILLLISPILAVLAIGVKLSSPGPVIFRQRRTGLDGEIIEVYKFRSMRTQD
                     NGPVVKQATRDDPRITPFGAFIRKTSLDELPQFVNVLQGRMSIVGPRPHAVAHNEQYR
                     RLVKAYMARHKVKPGITGWAQVNGLRGETDTLDKMAARVEYDLEYLRNWSLGLDLLII
                     ARTVKLVFVDHKAY"
     gene            14994..>15553
                     /locus_tag="VF453_03725"
     CDS             14994..>15553
                     /locus_tag="VF453_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686788.1"
                     /translation="MSNNAIACLLGLALAAGAGGAAAATGARITLQGDGVDLSLNQPA
                     DGANFTFGSFDVALAPGATVQETFDYTVRLSADGLPVARDWSFCTPITETDCGPDPTG
                     FEQAYASIWMGRDGRSPTDQDEWISDSLSFVNFLAPAGQSGLYTGQLVYTATNTSTLF
                     EQSTNVTILAAAFVDAAPVPEPAPLT"
BASE COUNT         2406 a         5704 c         5283 g         2160 t
ORIGIN      
        1 ccccgcaccg cctgagccgg ccgcgccggt cagcgcgcct gcgccacggc caggcagtgc
       61 gcggtgcgtt gcgcgaccac ggtctcgaag tcgttgcgaa cgccaaagcc gcgcgcccgc
      121 cgggccgcgt cggcgagctc cccgcgcgcg ccgtgcaggc gctcgacgac atcggccagg
      181 cgctcgatcg cgcccatggg cacgacccag ccgacgccgt gcgaggcgac gagggcggac
      241 agcgactcgt tggcgaagcc ggcgatcggc acgccgcagc ccagggtctc ggcataggtg
      301 cacgacggat cgccctggcg gtggcagcac acgaagaggt cgacgtcgcg ctgcagggtg
      361 ggcatcagga cgccggcgaa gtccacggga ccgttcaccc gcatggacga gcccaggccg
      421 aggcgcgcga tgtccgcgcg catgccggcc tccagttcgc cggcgcccca gacgtcgaag
      481 tgcaccggca cgccacgccg ctgcagcgcc gccgcgagcg gcaccagcgc atcggcaccc
      541 ttggccgcga tgaggcggcc cgagaaggcg agccggagcg gacgcggcgc cgcgagcgtc
      601 gccaggcgcg cctcgagcgc ggcctcgccc acgacgtcgg cggcggccag gcgcgtgtcc
      661 cagtacagca atccgtgggg gcgcccccgc gtataggcgt cgaaggcggc ctggccgttg
      721 gcctgcacgc cgtcgcactc gcgcagcgcg cggcggatgc ggggttcgtt gcgcaggtgc
      781 caggcaagcg tcttcaggcg cttgagcggc gtcatgggtg cctggcgcag catgtccagc
      841 cgggtgcgca gcgtgtactc gatcgccatc acgcaagccg tgccgacgcc gcggcagagg
      901 tcgggggcga cgagcttgcg cgggctgtcg gcgctggcca gcagcacgtc ggcgcccgcg
      961 atgtggtgac ggccgaaggc ctcgccgcgc gggatcagct cgagcgacca ggcctcgccc
     1021 gcgcccgtcc agcggcaggc gccgaatgcc ggcatggcgg gcaggtcgac gacgtccatc
     1081 accgccttga tccggccggg ccagcgggcg gcgaactcgt cctggccgtc cttgaacttg
     1141 cgatccagcc acagctcgcc gtcgatgcgc cacgcgggca ccgccggcca gacgaccagc
     1201 gtcggcccgg cggaaggggc tgcggcctcg cgggtcacca gccgtggctc gagagcaggg
     1261 acttcgacag cgcttccagc cgcccgcgga tggcgttgcc gtgcgcgttc atcaccgtcg
     1321 cggtctcccg gccgcgtgcc ttgaggtcgt cgaccagcgc cagcatctgg ccgacgtcca
     1381 gggcgtccga gcggacgcag aattcctgga cgcccatggt cgtcgcgaac tcgcggcact
     1441 tggggcggta ctcgatcatc agggacggaa cgctgctgca catcgccagc gcgacggcgt
     1501 gcagcttgat gccgacgaac acctgcatgc ggcgcacggc ctcctggaag cggcgcgcgc
     1561 cctcgtagat ctcgtggatc accggatcct tcacgccggc ctcggcggcg atgcggcgcg
     1621 tggtctcgag gtcctcgggc cagacgcaga agaactcgaa gcgatggccg cgtgcgccca
     1681 gctccctgac cagctgcgcg agctgcgtct ggatggtgct ctcctgccca tacatgtggc
     1741 catgcgagtg gccgacgttg acgccgatga ggccctcggt cggctgccag aagtccaccg
     1801 gccgcgtgaa ccaggccacc gggtcgccca gcacctgcgc gtccacgccg gcgcggcgca
     1861 aggtggcggc cgattcgggc ccgcggacgc ccacgtaggc gagcggccgc agcacctcgc
     1921 cccactgctc cagccacgag gggatcgggc cgtccaccgc cggcgtgacg cccgcgccga
     1981 agacgaacgc cgccttgctc gccgcgatcg cgcgccggaa gcggtcgatc ggcgagcgct
     2041 ccccgatctg cgtgccgccc cccagcatcg ccagctcgtg gcgtttgcgg gcgaccagcg
     2101 cgcgggcgcg ggcgttgtgg aactcgcgga tccagacgat gcgcgccggc gcgaagcatt
     2161 cctgcgccgc ctccaggagg atctcgtcgc cgaggttccg gtagcccacc cagccggtgt
     2221 agtggatgga atgcgagtcc tgcgcaaggt aacgcaggat gtcgccgatc tgaccgagct
     2281 tgctcatgga ggtgggtagg aaggatggaa tcgttgacgg aaggcgcgcg tgcgccggtc
     2341 gatcggccgg cattctgcgg caaatcggtg acgacgccgg tacaagccgc gcgcggcggc
     2401 gccggatgca gctatcgtgc ccggcgcagg ccgggcgcgc cgcctgccaa gggcgccact
     2461 catggcagaa tgcccgggtt ccacgcccgg gaagggtcgc ggcttccgga gagaccgatt
     2521 tcgggcaaca gcatgaacaa acttggggat tgtgggacac ggcaggacag ccgctggccg
     2581 cgccagccct tgctcaacgt ctgggcggac gacatcccga tggacgacct gctcgcgcgg
     2641 ctcgcgaccg agggcggcat cgtcttcacg gtcaaccccg accacctgta ccacttgcag
     2701 cacaacccgg cgttcctgca cgcctatcgc tgcgccgacg tgatcacggt ggacagccac
     2761 tacatccgca tcgtcctgcg gctgctgggg cgtccggtgc ggcaccgcct gcccggatcg
     2821 gacatcgtcc cggcgctgtg tcgccgccag gcccaggacc cggcgacgcg catcttcctg
     2881 ctcggcgcgc ggccgggcgt cgcgcagcgg gcgcgcgagg cgatcaacgc ccgcgccggc
     2941 cgcgagctgg tcgtcggcgc ccatgggcct tcgatgaatt tcgtcaatga tccggcggag
     3001 atcgatgcca cgatcgacat gatcaacgcg agcggcgcga cggtgctgct cgtgggcctg
     3061 ggcgcgccca agcaggagat ctggatcgcc agcgtgcgcc accgcctccc ccacgtccgg
     3121 gcgatgatgg gcgtgggcgc gacgatcgac tacgaggccg gcgaggtgcg tcgcgcgccg
     3181 cagctgatgc gcagcctcgg gctcgaatgg ttctaccgcg tggcgaccga gccggggcgg
     3241 tacctcatgc gctacgtccg cagctcccgc ttcatctggt ggatgctgat ggatcgcctg
     3301 ggccgctaca aggatccgtt cgccggcccg gcctccgcgt ccgtgcagtc ccgctgaccc
     3361 aagccgggcg cgcccgcacc gccgtgccgc ccgggacccg aacgaagaaa aggacgagac
     3421 gacccatgca actcaagccc gccttctcgc tgacgcccct cgtgctgtgc ctggccgccg
     3481 gttcggcgca tgccgacctg tcgccctact cgttcggggc ctcggagacg atccagcacc
     3541 agagcaacat cgaccacgga ccgggcaacg gccccggcga ctggctgtcg acgaccgagc
     3601 tgcgcggcgg gctggaccag ccgctgggcc gcgacgagct caaggccaac gtcgccatcg
     3661 acttcaaccg ctacaagaac ttccatcgcc gcaatgcgac gggctacctc gcgagcacgg
     3721 agttcgactg gagcctgccg gcggaccttt cgggctcgtt cggcgccgag acgcggcgcc
     3781 ggcagtacgt ctacggactg aacggcgagg ccctcggcgg cagcggtggc agcgaccagc
     3841 gcaacctcga gaccgacaac cacttctacg cccgcggcca gatcggcggg gagtcgcggt
     3901 ggaccctcta tggcggcctc gacgccacga accgtcgcta ctccctcgcc tcgttcagcg
     3961 gcaacgacga gcgccagtgg tccggcaacg tcggcacgcg ctacgcgacg agcccggacc
     4021 tgagcttctc gctgcaggcg tcgtactcgc acggcgaata ccccaacttc cagcagcagg
     4081 cgggcggccc cgtcgtcgac gacacgttca gcatgcgctc gtacgacctc gtcaccaact
     4141 acgtcgcctc gggcaacagc aagctcgacg cgagcgtcgg cttcaccacg cagaccagcg
     4201 acatccagtc cgcccgccat ttcgtcaacg ggtcgctgaa ctggacatgg acgccgccct
     4261 cgcacttcaa cgtgcgcgtc ggcctggccc gcgactcgga cgccgacacc gacgcggtca
     4321 acgtcagctt caacccgaac ggcatcaacg gtcgctccat caacaacgtg gcgcacgccg
     4381 acatcggcta cgagctgact gccaaggtga gcctcgacct gctggcgcag tacacccagc
     4441 gcaagtactc gaacgcggtg ctgcccgtcc tcgatgcgca gggcaagccg caggtcgcca
     4501 acggcaccaa caacacgcgg cgcgtgtacc tgtcggcgca ctaccagccg acgcgcacca
     4561 ccgacctgag ctgcggcgcc gggcgcgaga tccggcgctc ccacgtctcc ggcgacctga
     4621 cgccaggcta caccgacaac accgtgcagt gcgtcgccgc gatcgacttc aagtgaacgg
     4681 gccggccgcg tgccgcccac ggattcccgg attgaacagg aacgacaacg aatgaacacg
     4741 gtcgccgtcg tcggcctcgg ctacgtgggg ctgcccctcg tgatcgagtt cggcaagctg
     4801 ggccgcacca tcggcttcga catcgccgcg gacaaggtcg cgcgatgccg cgcgggcacc
     4861 gacccgtcgc gcgagatccc cgacgccgag atggcgctcg ccacccacgc cgagtacacc
     4921 agcgacccgg ccgacctgcg cgacgccgac gtcgtcatcg tcgcggtgcc gacgccggtc
     4981 gaccacgcgc acattcccga cttcggcccg ctgatcgggg cgtcgcgctc gatcggcccg
     5041 cacctgaagc ccggcgccac cgtggtctac gaatcgaccg tctaccccgg cgcgaccgag
     5101 gaggtctgca tccctgagct ggagcgcagc tccggcaagc ggtggaagcg cgacttcttc
     5161 gtcggctaca gccccgagcg catcaacccg ggcgaccgcg agcacacgct gacgcgcgtg
     5221 gtcaaggtcg tcgccggcga catgcccgag accctggagc gcgtcgccgc gctgtacgag
     5281 cgcatcgtgc agcccggcgt gcatcgctgc tccagcatca aggccgccga ggcctgcaag
     5341 gtcatcgaga acacgcagcg cgacctcaac atcgcgctga tgaacgagct cgcgatcatc
     5401 ttcgacaagg tcggcatcga cacgagcgag gtgctcgagg ctgccggcac caagtggaac
     5461 ttcctgaagt tcaagccggg cctggtcggc ggccactgca tcggcgtcga cccgtactac
     5521 ctcacgcaca aggccgagac gctcggctac cacccgcagg tgatcctcgc cggccgccgc
     5581 atcaacgacg gcatgggcaa gttcatcgcg gagcagaccg tcaagcagat gatcgccgcc
     5641 ggctcgtccg tcaagggcgc gcgcgtgaac gtgctgggcc tgaccttcaa ggaagactgc
     5701 gccgacctgc gcaacagcaa ggtcgtggac atcgtcgccg agttgaagtc ctacggcgtc
     5761 gaggtgttcg tgcacgaccc gcaggccgat gccgcggaag cgcgccacga gtacggcgtc
     5821 gagctggtgg cctgggacga cctgccgcgc gccgacgcgg tggtcgccgc ggtcgcccac
     5881 cgcgagttca aggcgctggg cgtcgaccgg atcgccgagc acctggtcgg cggcggcgtc
     5941 ttcatcgacg tgaaggcggc attccccgag ggcgagctgc tcgcccgcgg gctgcgcgtc
     6001 tggcgcctgt gacggccggg ggcggacgat gaaggtgctg gtcaccggcg cggccggctt
     6061 catcgggatg cacgtcgcgc agcgcctgct cgcgcgcggc gacgaggtgg tcggcatcga
     6121 cagcctcaac agctactacg acccggcgct gaaggcggcg cggctggcgg agctcgcgcg
     6181 cgacccgcgg gccgccgcgt tccgcttcga gcgcctggac gtcgccgacg gcgcggcgct
     6241 gcaggcgctg ttcgcgcgcg agcgcttcga ccgtgtcgtg catctcgcgg cgcaggccgg
     6301 cgtgcgctac tcgatcgaga atccgcaggc ctacggcgag gccaacctcg cgggcttcct
     6361 gaacgtgctc gaggcatgcc gccggcatcc agtcgaacac ctcgtctacg cgagcagctc
     6421 cagcgtctac ggcggcaacg ccaagatgcc gttctcggag gccgacagcg tcgaccaccc
     6481 ggtgagcctg tacgccgcga ccaagaaggc caacgagctg atggcgcaca cgtacagcca
     6541 cctgtacgcg ctcccgacga ccggcctgcg cttcttcacg gtctacgggc cgtggggacg
     6601 gccggacatg gcctacttct cgttcacgcg cgacatcctc gccggccggc cgatcgcggt
     6661 cttcaacgaa ggccgcatgc tgcgcgactt cacctacatc aacgacatcg tcgacggcgt
     6721 ggtcgcggtg ctcgacaagc ccgccgcgcc cgatcccggc ttcgagccgc tcgcgcccaa
     6781 tccgggcacg agccgcgcgc cgtaccgcgt gttcaacatc ggcaaccagg atccggtcgc
     6841 cctcggcgag ttcatcgcga cgctcgagcg cgcgctgggc gtcgaggcga tccgggatta
     6901 ccggcccatg cagccgggcg acgtcgtggc gacgcatgcc gacgtctcgg cgctgaaggc
     6961 gtggaccggc gtgtccccgc gcacgccgct tgccgacggg ctcgcccgat tcgtcgagtg
     7021 gtatcgtcgc tacttcgccg atcgcgcgtg agaacctaac cgccgcggcg cgcgccgacc
     7081 ggcgtccacc aggaaccatc gcatgattct cgtcaccggc ggcgccggat tcatcggcag
     7141 caacttcgtc ctcgactggc tcgagaacca cgacgagccc gtgctgacgc tggacgccct
     7201 gacctacgcg ggcaacctcg ccaacctgga gtcgctgcag ggcgacccgc ggcacgtctt
     7261 ccagcacggc gacatcggcg accgcgcgct cgtcgacgcg ctgctcgcca ggcaccagcc
     7321 gcgggcgatc gtgaacttcg cggccgagag ccacgtcgac cgctcgatcc tcggccccgg
     7381 cgccttcctc aagaccaaca tcgagggcac cttcacgctg ctcgaggccg cgcgcaccta
     7441 ccacgcgacg ctcgagggcg aggatcgcgc ggcgttccgc ttcctgcacg tgagcaccga
     7501 cgaggtctac ggctcgctca cgcccgacgc cgcgcccttc acggagaacc atccctacga
     7561 gccgaacagc ccgtactccg cgagcaaggc ggctagcgac catctcgtgc gcgcctggca
     7621 ccacacctac ggcctgccca cgctcacgac gaactgctcg aacaactacg gcccgctgca
     7681 cttccccgag aagctgatcc cgctggtgat cgtcaatgcg ctcgcgggca agccgctgcc
     7741 ggtctacggc gacggcctga acgtgcgcga ctggctctac gtgcgcgacc actgcgcggc
     7801 gatccgcgag gtgctcgcgc gcgggcgcgt cggcgagacc tacaacgtcg gcggcctcaa
     7861 cgagaagacc aacatcgaca tcgtgcgcgg catctgccgg ctgctcgacg agctgcggcc
     7921 cgatccggcc ggcccgcgcg agcgcctgat caccttcgtg aaggaccgcc ccggccacga
     7981 ccgccgctac gcgatcgacg cgaccaagct ctgcaccgag ctcggctggc gtccgcagga
     8041 gaccttcgag accggcctgc gcaagacggt gcagtggtac ctcgccaacg cggagtgggt
     8101 gcagggcgtg cagagcggcg cctaccggga ctgggtctcg acgcagtacg gcaagcactg
     8161 aggcgggcga cgcgatgaag atgctcctct tcggcgcggg cggccaggtc ggctgggagc
     8221 tccagcgctc gctgcagccg ctcggcgagc tggtggcgct ggacttcgac agccgcgacc
     8281 ttccggccga tttcagcagg ccggagtcgc tcgccgcgac ggtcgaggcc gtgcggccgg
     8341 atgtcatcgt caacgccgcc gcccacacgg cggtcgacaa ggcggagtcc gagcccgcac
     8401 tggcgcaggc gatcaacgcg gacgcgcccg gcgcgatcgc gcgcgcggct gcggcctcgg
     8461 gcgcgtggct ggtgcactac ggcacggact acgtcttcga cggctcgggc gaccaaccgc
     8521 gcgacgagac ggcgcccacg gccccgttgt cggtctacgg ccgcacgaag ctggccggcg
     8581 aggagcagat ccgcgcgagc ggctgccgcc acctgatcct gcgcacgagc tgggtctacg
     8641 ccgcgcgcgg cggcaacttc gcgcgcacga tgctgcgcct ggcgggcacg aagaccgagc
     8701 tcagcgtcat cgccgaccag gtcggcgcgc ccacgggcgc cgacctgctg gccgacctca
     8761 gcgcccacat gatccgcgcc gcccgcgcca cgccggagct ggccggcacc taccacgcgg
     8821 tcgccggcgg cttcacgagc tggcacggct acgcgaccct cgtgatcgag acggcgcgcg
     8881 gccgcggcat gccgttgacc gtgcccgccg gcgcgatccg cccgatcccg acgagcgcct
     8941 acccgacgcc cgcgccgcgg ccgctgaact cgcgcctgga cacgcgcaag ctgcgcgagc
     9001 gcttcggcct gtcgctgccg ccctgggaag ccggcgtgcg ccgcatgctc gacgaaatcc
     9061 tcccggcctg agaatccgag gccccgttct cgaagggaac gcatgaaccg caaaggcatc
     9121 atcctcgccg gaggctccgg cacccggctg catccggcga cgctcgcgat cagcaagcag
     9181 ctgatccccg tctacgacaa gccgatgatc tactacccgc tcagcgtctt gctgctggcg
     9241 ggcatccgcg agatcctcgt gatcagcacg ccgcaggaca cgccgcgctt cgagcagctg
     9301 ctgggcgacg gccgccgctg gggcctcgag ctgcgctacg tggtgcagcc gagcccggac
     9361 ggcctcgcgc aggcgttcct gatcggcgag gacttcctcg gcggcgcccc gagcgctctc
     9421 gtgctgggcg acaacatctt ctacggccac gacctcggcg gccggctgga ggccgccaac
     9481 gcgcgcgccg agtgcgccac cgtcttcgcc tacccggtgc acgatcccga gcgctacggc
     9541 gtcgtcgagt tcgacgccgg cggccgcgcg atcagcctcg aggagaagcc ggccaggccc
     9601 aagagccgct acgccgtgac gggcctgtac ttctacgacg aggacgtcgt cgcccacgcg
     9661 aaggcgctcg cgcccagccc gcgcggcgag ctcgagatca ccgacctgaa ccggcgctac
     9721 ctcgaggccg gcaagctcga cgtccagatc ctcggccggg gcgatgcctg gctcgacacg
     9781 ggcacgcacg actcgctgct cgaggccagc cagttcgtgc agacgctcga gaagcgccag
     9841 ggcctgaagg cctgctgccc ggaggagatc tgctggcgcc agaagtggat cgacgacgcg
     9901 cagctcgccg cgctcgccgc gccgctggcc aagagcggct acgggcaata cctgcagcgg
     9961 ctcctctcgg agcgcgtgtt ctgatgaacg tcgagaccac cgccatcgag ggcgtgctga
    10021 tcctgcagcc gaaggtcttc ggcgacgcgc gcggattctt cctcgagagc ttcaaccaga
    10081 aggtcttcga cgaggccgtc ggccgtcacg tcgacttcgt ccaggacaac cactcgcgct
    10141 cggcgcgcgg ggtgctgcgc gggctgcatt tccagcgtgg cgagaaggcg cagggcaagc
    10201 tggtgcgcgt cgtcgccggg gcggtgttcg acgtcgcggt ggacatccgg cgcgacagcc
    10261 cgagcttcgg gcgctgggtg ggcgtcgagc tgtcggccga caaccaccgc cagctgtggg
    10321 tgccgccagg cctcgcgcac ggcttcctgg tgctgtccga gacggccgac ttcctctaca
    10381 agacgaccga gttctactcg ccgcagcacg agggtgcggt gcgctgggac gacccggacc
    10441 tcgcgatcgc ctggcccgac gtgggcgcgg caccgaccct ctcggccaag gacgcggccg
    10501 ccggcacctt gcgggacctg ccgtgagcct ccggccgagc aggccgaccg catgaatcgc
    10561 ctcgcatgac gcggcgcccg cctacatcgc ccgtcatggc cgcgctgcca gaatgcggcg
    10621 gcgcgatgtg tggcgccgct gcaccgcccc tgtcagcggg attcccggac gcctcgtgcg
    10681 cgccggatac tcgctcgacg tcgccggaca accatcgatc aaaggatttg ctatggccaa
    10741 ggccatgatt ctcgccgccg ggcagggcac ccgcgtgcgg ccgctgacca agaacctgcc
    10801 gaagccgatg atcccgatcc tcggcaagcc cgtgctggag tacctgatcg agcacctcgc
    10861 gcgctacggc gtcaacgaga tcatgatcaa cgtggcctac acgcactgga agatcgagaa
    10921 ctacttcggc aatggctcgc gctggggcgt ggagatcggc tactcgttcg agggcacgcg
    10981 cgaccacggc gacatcgtcc ccaagccgat gggctcggcc ggcggcatgc gcaagatcca
    11041 ggaccacagc ggcttcttcg acgagaccac gatcgtgctg tgcggcgacg cggtcatcga
    11101 cctggacatc ggcgcggccc tccacgagca caagaccaag caggcgctcg cgagcgtcgt
    11161 gacgctggag gtgcccgagg aagaggtcaa gaactacggc gtggtcgtga ccgacaagga
    11221 cggccgcatc accgaattcc aggagaagcc ctcgccggcc gaggccaagt cgcgcctcgc
    11281 gagcacgggc atctacatct tcgagccgca ggtgctcgac ctgatcccgt ccggccagac
    11341 cttcgacatc ggcagccagc tgttcccgct gctggccgag cgcggcctgc cgttcttcgc
    11401 gcagagccgc tggttcaact ggatcgacat cggccgcgtc accgactact gggcggtctg
    11461 ccagcgcgtg ctgcgcggcg aggtggcgca gatggacatg ccgggccgcg aggtgcgccc
    11521 gggcgtctgg gtcggcctga acaccagcat cgacttcgac aaggtcgaca tcacgggacc
    11581 ggtctacatc ggctcgtcca gcgtcatcga gccgggcgcc aaggtcgtcg gcccggcgtg
    11641 gctgggcaac ggctgcatcc tgcggcaggg cagcaagctg gtgcgcagca tcctgttcga
    11701 ctacgttcgc ctttcgcagg gcgagaccct ggaggatatg atcgtctcgc cctactactg
    11761 cgtcgaccgc cacggcaaca cgacctatcc cggcgacgag agcaagccgc tgcgctgggg
    11821 cgacgcccgc gcctcctgac gcccgagaat cccccgggcg cgccgtctac ggagggcgcg
    11881 cccgcttcca tttcccaccg ccagagaatc ccgatgtcca cggccatccc gatccaaccc
    11941 gtcatcatgg ccggaggcag cggcacgcgg ctgtggccgc tgtcgcgcgc gggcttcccg
    12001 aagcagttcc tcgcgctggc gggcgaccgg agcctcttcc aggaggcggt ggcgcggctg
    12061 gccggcctcg cggccggcga catcgccgta cgtgcgccgc tggtggtggg caacgccgag
    12121 catcgtttcc tcgcgctgga gcagttgcgc gagcagggcg tcgagccggc gtcggtgatc
    12181 ctcgagccgg tcggccgcaa caccgcgccg gcgctgacgc tggccgcgct cgcggcgacc
    12241 gagggcgacg ccgatccggt gctggtcgtc tcgcccgccg accagaccgt caccgacgtc
    12301 cccgccttca ccgcggccct gcagcaggcg gtgcgcggcg ccgccggcgg cgcgatcgtg
    12361 atcctgggca tcacgcccga ccgcccggag acgggctacg gctacatccg cagcgtcgcg
    12421 ggcgacgacg gcctggcccg cgtcgcgcag ttcgtcgaga agccggacgc cccgaccgcg
    12481 gcgcgctacc tcgacgaggg cggctacacc tggaactcgg gcatgttcgt gctgcgggcg
    12541 tcgacctggc tgaaggcgct ggaggcgttc cgccccgaca tcctcgccgc cacccgcgcc
    12601 gcgtggcagg gccgcgagcc cgacgcccgc ttcgttcgcc cgacgcgcga ggccttcctc
    12661 gcggtaccgg cggagtcggt cgactacgcc gtgatggaac gctgccccgg cagcgcgttc
    12721 gacatccgca tggtgcccct ggccgccggc tggaacgacc tcggcgcctg ggacgccgtc
    12781 tggcaggtcg cgtccaagga cggcgcgggc aacgtggccg tcggcgacgt gatgttcgag
    12841 gacagccgca acacgctggt ccacgcgagc ggccgcctcg tgaccaccgt cggcctggac
    12901 gacgtcgtca tcgtcgagac ggcggacgcg gtgctggtcg ccgaccgcgc gcgcagccag
    12961 gacgtcaaga agctggtggg ccgcctggac agcggccggc gcaccgagca tgcgctgcat
    13021 cgcaaggtcc accgcccctg gggctggtac gacagcatcg acgccggccc gcgcttccag
    13081 gtcaagcgca tcatggtcaa cccgggcgcg tcgctgagcc tgcagatgca ccaccatcgg
    13141 gccgagcact ggatcgtcgt gcagggcacc gccgaggtca cctgcggcgc gcgcaaagtc
    13201 atcctgaccg agaaccagag cacatacatc ccgctcggcg agacgcaccg gctcgcgaac
    13261 ccgggcaaac tgccgctcga gatcatcgag gtgcaatccg gctcctatct cggcgaggac
    13321 gacatcgtcc gcttcgagga cacctacggc aggaattgat ggcccccacg ctcgctggcg
    13381 cttgctgcct cccggtgcgc gcctgcgcat ccccggccgt tggcccgaat cccgcttcag
    13441 atcgcgcccg accttccacc tgacatgttc gaagagcgca gcgtccgcaa gcagttcctg
    13501 agcgcgccgc cgtccatcgg cagcgtgatc gcgtccctgc tcgagcccct ggtggccgcg
    13561 ggggcgctgg ccgtcttcca cgcgatctac ggctaccgca tggacggcgc ggcgatggcg
    13621 ctggccatcc tgctgctggt gctgatgttc ccgggcgtaa accgcttcgg gcgcacgggc
    13681 gtcggcgtcg gcatcgacat catcctgtcg tgggtctggg tgctgacggt gctggcgctg
    13741 ctgggctacg cgaccgacag cctgaacgcc ttcgacccgc gcatgctgac cggctgggcg
    13801 atcgcgacgc cgctcgtgca gtgggcgctg gtggcgatcg gcacggcgat ccagcgccac
    13861 ctcgcctcgt tgccggacgc gcgccggccg gccgtgatca tcggcgccgg ccgcatgggc
    13921 atgcgcgtcg cgcagatgct gcgccagcgg cgctccttcg gccacgacct gctgggcttc
    13981 ttcgacgacc gctccgccga ccgcgtcaac ctgccgccgg acgcgctgct gctcggcgcg
    14041 ctcaagggcc tgcccgagta catcgaggtg cacggcgtca aggacgtctt catcacgctg
    14101 ccgctgacct cgcagccgcg catccagtcg ctgctggagt cgctgcagaa caccaccgcg
    14161 tcgatccact tcgtgcccga catcttcggc gtcagcgtga tccagggccg gctcgaggac
    14221 atgggcggcg tgccggtcgt gggactgatg gtggcgccgt tcacgggcat caacggcttc
    14281 gtcaagcggg cgagcgacat cgtgctgtcc gggctgatcc tgctgctgat ctcgcccatc
    14341 ctcgcggtgc tggccatcgg cgtgaagctc agctcgccgg ggccggtgat cttccgccag
    14401 cgccgcaccg gcctggacgg cgagatcatc gaggtctaca agttccgctc gatgcgcacg
    14461 caggacaacg ggccggtcgt caagcaggcg acccgcgacg atccgcgcat cacgcccttc
    14521 ggcgcgttca tccgcaagac ctcgctcgac gagctgccgc agttcgtcaa cgtgctgcag
    14581 gggcgcatga gcatcgtcgg gccgcggccg catgccgtcg cgcacaacga gcagtaccgg
    14641 cgcctggtca aggcctacat ggcgcgccac aaggtcaagc ccggcatcac gggctgggcc
    14701 caggtcaacg ggctgcgcgg cgagaccgac acgctggaca agatggccgc gcgcgtcgaa
    14761 tacgacctcg aatacctgcg caactggtcg ctggggctcg acctgctgat cattgcgcgc
    14821 acggtcaagc tcgtcttcgt cgatcacaag gcctactgag cggccgagcc gcgcgaacgc
    14881 ggccgcattt gtcacagcgg acgagcgaat tcctacgttc gctttcgatt ttggctgcgg
    14941 ccgcgtggcg tccctcttct aagctcgctg gaattccacg acggagacaa gggatgtcga
    15001 acaacgcgat cgcctgcctg ctgggcctcg cactggccgc cggcgcgggc ggcgccgcgg
    15061 ccgccacggg cgcgcggatc acgctgcagg gtgacggggt cgacctctcg ctgaaccagc
    15121 cggccgacgg cgcgaacttc accttcggca gcttcgacgt cgcgctcgcg cccggcgcga
    15181 cggtgcagga gaccttcgac tacaccgttc ggctgagcgc cgacggcctg ccggtcgcgc
    15241 gcgactggtc gttctgcacg ccgatcaccg agaccgactg cggacccgac ccgaccggct
    15301 tcgagcaggc ctatgcctcg atctggatgg gccgcgacgg gcgctcgccg accgaccagg
    15361 acgagtggat cagcgactcc ctgagcttcg tcaacttcct cgcgcccgcc gggcagtccg
    15421 gcctgtacac gggccagctc gtctacaccg cgaccaacac ctccacgctg ttcgagcagt
    15481 ccacgaacgt gacgatcctg gcggcggcct tcgtcgatgc ggccccggtg ccggagcccg
    15541 cgccgctgac gat
//
LOCUS       DASVLN010000162         5748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_170026, whole genome shotgun sequence.
ACCESSION   DASVLN010000162 DASVLN010000000
VERSION     DASVLN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170026"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1377
                     /gene="odhB"
                     /locus_tag="VF453_03730"
     CDS             121..1377
                     /gene="odhB"
                     /locus_tag="VF453_03730"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX7686789.1"
                     /translation="MALVEVKVPQLSESVAEATMLQWKKKVGDPVAIDEILIEIETDK
                     VVLEVPAPAAGVIAELTVGDGATVAAEQLIARIDTEGKAVAAAPAPIAAAAAAVAVPA
                     PVATATGGAMAGVAMPAAAKLMADNQLAAGSVPGSGRDGRVTKGDVLGAVAAGATKPV
                     AAIPIPTGAPVKLLPQVAAPAVKLGDRPEQRVPMSRLRARVAERLLQSQATNAILTTF
                     NEVNMAPVMEMRKRFQEKFEKEHGVKLGFMSFFVKAAVAALKRYPVINASVDGNDIVY
                     HGYFDIGIAVGSPRGLVVPILRNADQLTFAEIEKKIAEFGQKAKDGKLSLEELTGGTF
                     SISNGGVFGSMLSTPIINPPQSAILGVHATKDRAVVENGQIVVRPMNYLAMSYDHRII
                     DGREAVLGLVAMKDALEDPSRLLFDI"
     gene            1537..2964
                     /gene="lpdA"
                     /locus_tag="VF453_03735"
     CDS             1537..2964
                     /gene="lpdA"
                     /locus_tag="VF453_03735"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7686790.1"
                     /translation="MAKNFDVVVIGGGPGGYIAAIRAAQLGFNVACVDEWKNDKGGPA
                     PGGTCTNVGCIPSKALLQSSEHFEHAGHAFADHGIQVSGLAIDVAKMLARKSAVVKQN
                     NDGILYLFKKNKVAFFHGRASFAKAEGGAYELKVAGAAEETLVARQVIVATGSNARAL
                     PGAAFDEDKILSNDGALRIGAVPATLGVIGSGVIGLEMGSVWRRLGAQVTILEALPAF
                     LGAADESIAKEAGKVFAKQGLKIELGVKVTKVDTAGEGVKVSYTDAKGGEQVLAVDRL
                     IVSIGRVPNTIGLNPEAVGLKLDERGAIVVDDECRTNLPGVWAIGDVVRGPMLAHKAE
                     EEGVAVAERIAGQHGHVNFNTVPWVIYTSPEIAWVGQTEAQLKAAGRDYKAGQFPFLA
                     NGRARALGDTTGFVKVIADAKTDEILGVHMIGPMVSELISEACTAMEFKASSEDIARI
                     CHAHPSLSEATKEAALAVDKRTLNF"
     gene            3062..4162
                     /gene="zapE"
                     /locus_tag="VF453_03740"
     CDS             3062..4162
                     /gene="zapE"
                     /locus_tag="VF453_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX7686791.1"
                     /translation="MPTVTELYHQTLEERGFRADEAQLAAVAALQRCEDEWVAYKSRR
                     SNAITKMLVRPPIPRGVYMYGGVGRGKSFLMDCFFQAVPLKRKVRLHFHEFMREVHRE
                     LHELKGMADPLQELGRRISLRYRLICFDEFHIADVTDAMILHRLLLALFDNRVSFVTT
                     SNFRPDDLYPNGLNRERILPAIELLNARLEVINVDAGTDYRQRTLTDAKLYHTPLGPQ
                     ADAEMAAIFDHLAEARDEDPLLHIEHREIRARRRAGGVVWFDFRQLCGGPRSMNDYLE
                     LATQFHTLMLSDVPQMSPRTASEARRFTWLVDVLYDRRVKLVLSAAVPPEELYTEGPL
                     AHEFPRTISRLHEMQSKEYLEIDKRDVDTGLT"
     gene            4470..5672
                     /locus_tag="VF453_03745"
     CDS             4470..5672
                     /locus_tag="VF453_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX7686792.1"
                     /translation="METLKQSGDTLFLLLGAVMVLAMHAGFAFLELGTVRKKNQVNAL
                     VKILVDFAVSTIVYFFLGYTIAYGTSFLTGADQLALHHGFELVRFFFLLTFAAAVPAI
                     VSGGIAERARFEPQLAATAVIVGVAYPLLEGTAWNGRYGVQAWLKSTFGAEFHDFAGS
                     VVVHAMGGWVALAAVLLLGPRSNRYRKDGAISAHPPSNIPFLALGAWVLTVGWFGFNV
                     MSAQTLDKISGLVAVNSLMAMVGGTLVAVLMGRNDPGFAYNGPLAGLVAVCAGSDIMH
                     PAAALVVGGVAGAIFVQLFTLTQNRWKIDDVLGVWPLHGLCGAWGGIAAGIFGSSALG
                     GLGGVSFASQLVGTLATLAWGLASGFVVYGLLRRFVGLRLTPEEEFEGADLTVHRIGA
                     TPEREASW"
BASE COUNT          914 a         1942 c         1969 g          923 t
ORIGIN      
        1 ggctttgtgc tgaccaaata acttcggtct gtcattccag agagcgggaa tccgcgtcgt
       61 gcgtcgtggg taccggcgct tgcgggaatg gcagcctaaa aaccaacaga aagaattcaa
      121 atggcactcg tagaagtcaa agttccgcaa ctgtccgaat cggttgccga agcaaccatg
      181 ctgcaatgga aaaagaaggt cggcgacccg gtcgcgatcg atgaaattct gatcgagatc
      241 gaaaccgaca aggtcgtgct ggaagtccct gcgcctgcgg ccggcgtgat tgccgagttg
      301 acggttggcg acggcgccac ggtcgccgcc gagcagctga tcgccagaat cgacactgaa
      361 ggcaaagcgg ttgctgccgc gccggcacct attgccgcag ctgcggcagc ggtggccgtg
      421 cctgcgccgg tcgccaccgc aacggggggc gccatggcgg gtgtcgccat gccggcggct
      481 gccaagctca tggccgacaa ccagctggcg gccggctccg tgcccggcag cggcagggac
      541 ggccgcgtca ccaagggcga cgtgctcggc gcggtggccg caggtgctac aaaacctgta
      601 gctgctatcc caatacccac gggggctccg gtcaaattat tgcctcaagt ggcggcgccg
      661 gccgtgaaac tgggagatcg ccccgagcag cgtgtgccga tgagccgcct gcgcgcgcgc
      721 gtggccgagc ggctgctgca gtcgcaagcg accaatgcca tcctgaccac cttcaatgaa
      781 gtcaacatgg cgccggtgat ggaaatgcgc aagcgcttcc aggagaagtt cgagaaggag
      841 cacggcgtca agctcggctt catgagcttc ttcgtgaagg ccgcggtcgc cgcgctcaag
      901 cgctatccgg tgatcaacgc ctcggtcgac ggcaacgaca tcgtctacca cggctacttc
      961 gacatcggca tcgcggtggg ttcgccgcgt ggcctggtgg tgccgatcct gcgcaacgcc
     1021 gaccagctga ccttcgcgga gatcgagaag aagatcgccg agttcggcca gaaggccaag
     1081 gacggcaagc tctcgctcga ggagctgacc ggcggcacgt tctcgatctc caacggcggc
     1141 gtgttcggct cgatgctgtc gacgcccatc atcaacccgc cgcagtccgc catcctgggc
     1201 gtgcacgcga ccaaggatcg cgcggtggtg gagaacggcc agatcgtcgt gcgcccgatg
     1261 aactacctcg cgatgtccta cgaccaccgc atcatcgacg gccgcgaggc cgtgctgggg
     1321 ctggtggcga tgaaggacgc gctggaagat ccgtcgcgcc tgctgtttga catctagccc
     1381 ccacgcccgc tggcgcgggc tgccccccaa gggggcgttc gcgtgcagac cggcggagcc
     1441 ggatctgccg cttccccgtg tctctcttga cacgcgcgag cggcagcgcc ggatccgggt
     1501 agcgagcctg tcggctcgat tttttgggaa ctatccatgg ccaagaattt tgatgtcgtc
     1561 gtgatcggcg gcggccccgg cggctacatc gcggcgatcc gcgcggcgca gctgggcttc
     1621 aacgtcgcct gcgtcgacga gtggaagaac gacaagggcg gcccggcccc gggcggcacc
     1681 tgcaccaacg tcggctgcat cccgtccaag gcgctgctgc agtcgtccga gcacttcgag
     1741 cacgccgggc acgccttcgc cgaccacggc atccaggtct cgggcctcgc gatcgacgtc
     1801 gcgaagatgc tggcgcgcaa gagcgccgtc gtgaagcaga acaacgacgg catcctctac
     1861 ctgttcaaga agaacaaggt cgccttcttc catggccgcg cgagcttcgc gaaggccgag
     1921 ggcggcgcct acgagctcaa ggtcgccggc gcggccgagg agacgctggt cgccaggcag
     1981 gtgatcgtcg ccaccggctc caacgcccgc gcgctgcccg gcgcggcctt cgacgaggac
     2041 aagatcctct cgaacgacgg cgcgctgcgc atcggcgcgg tgcccgcgac gctgggcgtg
     2101 atcggctcgg gcgtgatcgg cctggagatg ggctcggtct ggcgccgcct gggcgcgcag
     2161 gtcaccatcc tcgaggcgct gccggccttc ctcggcgccg ccgacgagtc catcgccaag
     2221 gaggccggca aggtcttcgc gaagcagggc ctgaagatcg agctcggcgt gaaggtgacc
     2281 aaggtcgaca cggcgggcga gggcgtgaag gtctcctaca ccgatgccaa gggcggcgag
     2341 caagtgctgg cggtcgaccg gctgatcgtc tcgatcggcc gcgtgcccaa caccatcggc
     2401 ctgaatcccg aggcggtggg cctgaagctc gacgagcgcg gcgccatcgt cgtcgacgac
     2461 gagtgccgca ccaacctgcc gggcgtgtgg gcgatcggcg acgtcgtgcg cggcccgatg
     2521 ctcgcgcaca aggcggagga agagggcgtc gcggtggcgg agcgcatcgc cggccagcat
     2581 ggccacgtca acttcaacac ggtgccctgg gtcatctaca cgagccccga gatcgcgtgg
     2641 gtcggccaga ccgaggcgca gctcaaggct gccggccgcg actacaaggc gggccagttc
     2701 ccgttcctcg ccaacggccg cgcccgtgcg ctcggcgaca cgaccggctt cgtcaaggtc
     2761 atcgccgacg cgaagaccga cgagatcctg ggcgtgcaca tgatcggccc gatggtctcg
     2821 gagctgatct ccgaggcctg taccgcgatg gagttcaagg cctcgtccga ggacatcgcg
     2881 cggatctgcc atgcgcatcc gtcgctgtcg gaggccacga aggaagcggc gctcgccgtc
     2941 gacaagcgga ccttgaactt ctgatcccgg ccgcgtccag atgcgcggca atcacgggcc
     3001 gacggcaccg ctctcgaggc ggcccgccgc ggcccgtttt cattgaccct ctgcgatccc
     3061 gatgcccacg gtcaccgagc tatatcacca gacgctggaa gagcgcggct tccgggccga
     3121 cgaggcgcag ctcgccgcgg tcgcggccct gcagcgctgc gaggacgagt gggtcgccta
     3181 caagtcgcgc cgctccaacg cgatcacgaa gatgctggtg cggccgccga taccccgtgg
     3241 ggtgtacatg tacggcggtg tcggccgcgg caagagcttc ctgatggact gcttcttcca
     3301 ggccgtgccg ctcaagcgaa aggtgcgcct gcacttccac gagttcatgc gcgaggtgca
     3361 ccgcgagctg cacgagctca agggcatggc cgatccgctg caggagctcg gccgccgcat
     3421 ctcgctgcgc taccggctga tctgcttcga cgagttccac atcgccgacg tcaccgacgc
     3481 gatgatcctg caccggctgc tgctggcgct gttcgacaac cgcgtctcct tcgtcacgac
     3541 ctcgaacttc cggcccgacg acctctaccc gaacggcctc aaccgcgagc gcatcctgcc
     3601 cgcgatcgag ttgctgaacg ccaggctgga ggtgatcaac gtcgacgccg gcaccgacta
     3661 ccgccagcgc acgctgacgg acgcgaagct ctatcacacg ccgctggggc cgcaggccga
     3721 cgccgagatg gcggcgatct tcgaccacct cgccgaggcg cgcgacgagg atccgctgct
     3781 gcacatcgag catcgcgaga tccgcgcccg ccgccgcgcc ggcggcgtgg tctggttcga
     3841 cttcaggcag ctgtgcggcg ggccgcgctc gatgaacgac tacctggagc tcgcgacgca
     3901 gttccacacg ctgatgctgt cggacgtgcc gcagatgtcc ccgcggacgg cgtccgaggc
     3961 gcggcgcttc acgtggctcg tggacgtgct ctacgaccgg cgcgtcaagc tggtgctgtc
     4021 ggccgcggtg ccgcccgagg agctctacac cgagggcccg ctcgcccacg agttcccgcg
     4081 cacgatctcg cgcctgcacg agatgcagtc gaaggagtac ctggagatcg acaagcgcga
     4141 cgtcgacacc ggcctcacct gatcccacgg gctcgatcgc actggccgag ccttgcagcc
     4201 gcgccgccgg ccatcctata ctgttcgttc atacagcatt cgatcgagcc gatgtttccc
     4261 cgccgacctg ccggcgcacg tccgatgtgc gtcgagtcct tgaattggtg cgtcgatgca
     4321 ctgcaacggt gagtccgtgc gcgccgcaac gacgaatcgt ccctttccgc ccgccccaag
     4381 gccgagcacg cattttgctt ttcggtgcat cttgcgcgag ctcgcaccac agtggaatgc
     4441 gggaggcgct ggccagccga ggacgatgga tggagacttt gaagcagagc ggcgacacgc
     4501 tcttcctgtt gctgggcgcc gtgatggtgc tcgcgatgca cgccgggttc gcgttcctgg
     4561 agctgggcac ggtccgcaag aagaaccagg tcaacgcgct ggtcaagatc ctcgtcgact
     4621 tcgcggtctc gaccatcgtc tacttcttcc tcggctacac gatcgcgtac gggacgagct
     4681 tcctgaccgg cgccgaccag ctcgcgctgc accacgggtt cgagctggtg cgcttcttct
     4741 tcctgctgac cttcgcggcg gctgtgccgg cgatcgtttc cggcggcatc gcggagcgcg
     4801 cccgcttcga gccgcagctc gcggccaccg cggtcatcgt gggcgtggcc tatccgctgc
     4861 tcgagggcac ggcctggaac ggtcgctacg gcgtgcaggc gtggctgaag tcgaccttcg
     4921 gcgccgagtt ccacgacttc gcgggcagcg tggtcgtcca tgcgatgggc ggctgggtgg
     4981 cactggcggc cgtgctgctg ctcggaccgc gttccaaccg ctaccgcaag gacggcgcga
     5041 tcagcgcgca cccgccgtcc aacatcccct tcctcgcgct cggcgcctgg gttctgaccg
     5101 tgggctggtt cggcttcaac gtgatgagcg cgcagacgct ggacaagatc tccggcctcg
     5161 tcgcggtgaa ctcgctgatg gcgatggtcg gcggcaccct ggtggcggtg ctgatgggcc
     5221 gcaacgaccc gggcttcgcc tacaacggcc cgctcgcggg gctggtggcg gtgtgcgcgg
     5281 gctcggacat catgcatccg gcggcggcgc tggtcgtggg cggcgtggcc ggcgcgatct
     5341 tcgtccagct gttcacgctc acgcagaacc gctggaagat cgacgacgtc ctgggcgtgt
     5401 ggccgctgca cggcctgtgc ggcgcctggg gcggcatcgc ggccgggatc ttcggctcga
     5461 gcgcgctcgg cgggctgggc ggcgtgtcct tcgcgtcgca gctggtgggc acgctcgcga
     5521 cgctggcctg gggcctcgcg tctggcttcg tcgtctatgg cctgctgcgc cggttcgtcg
     5581 gcctgcgcct gacgcccgag gaggaattcg agggcgccga cctcacggtg catcgcatcg
     5641 gggcgacgcc ggagcgcgaa gcgagctggt gagtggtcgc ccggcagccg ccgggagagc
     5701 cgtcccgttc agggcgaccg ggacgggcgc gatcgacgcg cggcggcg
//
LOCUS       DASVLN010000163         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_170062, whole genome shotgun sequence.
ACCESSION   DASVLN010000163 DASVLN010000000
VERSION     DASVLN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170062"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="VF453_03750"
     CDS             <1..1761
                     /locus_tag="VF453_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7686793.1"
                     /translation="WLRDDGALSGEDVDKLLRRFGTSASSLHPLQRLGGASLVDARDG
                     RVLDTDGLARWLAARFELPYQRIDPLRVDVGRVSEVMSVAYAEMRRALPLNVGPTEVV
                     IGTSEPFDIAWVPEIEAHVKRPVRLQLAHPEDITRYTGEFYSLAAQVRVATRAQDAAN
                     AANSFEQLVELGKNKGSLDANDQGVVQVVDWLWQYAFEQRASDIHLEPRRDMGVIRLR
                     IDGVLHTAHQVPAAVMNAMTARIKLLGRMDVIERRRPQDGRIKTRRPDGAGGMPGGEV
                     EMRLSTLPTAFGEKVVMRIFDPGTVDKPFDALGFSPAEAERWQSLIGQAHGIILVTGP
                     TGSGKTTTLYSTLKKLATDEVNVSTVEDPIEMIEPSFNQTQVQPAVELDFASGLRALM
                     RQDPDIIMVGEVRDLETAEMAIQAALTGHLVFSTLHTNDAPSSITRLVDLGVPPYLIS
                     ATVIGVLAQRLARTLCALCKAPDDNADTVRMLEELAAPWRLGGRIRPMKAVGCPACRR
                     TGYRGRAALYELMPVTEPLKETIHPALDVPRLTRQAMVEGMRPLRLAGLMKVAEGLTT
                     VDEILRCTPQWTAARAAPAS"
     gene            1884..2462
                     /locus_tag="VF453_03755"
     CDS             1884..2462
                     /locus_tag="VF453_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7686794.1"
                     /translation="MKVRVLVAACVAALSAGANAAVVLSDNFDADAQGLNVVPAGWTV
                     ANAGTVDVIGTGFFDEIPGNGNYIDLDGSNGQAGLLTTSTGLLNGTYTATFQLGGNHR
                     DGTTDLVTVQFGGASAVFSVGPNDAFTTYSLTTTANLEQLSLSFLDGRGGNVGALLDN
                     VSISSAVPEPAGLPLLAAGVLGVAFAARRRRA"
     gene            2615..3571
                     /locus_tag="VF453_03760"
     CDS             2615..3571
                     /locus_tag="VF453_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7686795.1"
                     /translation="MSTDSIPPSPTLATVATTTPVTRPARLWPGLALATAGSIAFSGK
                     AIIVKLAYRHGVDAVTLIMYRMLFALPLFAALAWWAGRGRPALTGRDWRAIAGLGLTG
                     YYLASFLDFAGLAYVSAGLERLVLYLNPTLVLLLGRVLFKRRVAARQLGALAVSYAGV
                     LLVFGHEAHLAGGHVVLGTLLVFASAVSYAVYMTFSGEEVRRLGSLRLTGLASTVACA
                     LCIGQFLLLRPLAAARVDDAVIGLSVLNAVACTVAPVLMVMMAIERVGATVAAQTGMV
                     GPLSTILMGVAVLGEPFTAWIAAGTACVLSGIWLLARARPAT"
     gene            complement(3589..4068)
                     /locus_tag="VF453_03765"
     CDS             complement(3589..4068)
                     /locus_tag="VF453_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7686796.1"
                     /translation="MPGALAERIVDDKAWKLIDALQADARASLKTLAAAAGLSIAATA
                     ERLKRLQDAGVLRGTRAEIDAALAGWPVRAIVGITVLQPGKKALLDKLRRSAEVLECH
                     HVAGADSYLFTVVARDLPDLERFLGTINGYGETRTSIVFSTPIERRGLVRPGQARKR"
     gene            4162..>4368
                     /locus_tag="VF453_03770"
     CDS             4162..>4368
                     /locus_tag="VF453_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019452026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7686797.1"
                     /translation="MGLSTILQGAGIGFAIAAPVGPIGLLCIRRTLRDGPAAGLATGF
                     GAATADGVYGLVAAGGLGVASWLLG"
BASE COUNT          578 a         1596 c         1571 g          623 t
ORIGIN      
        1 tggctgcgcg acgacggcgc gctctcggga gaggacgtcg acaagctgct gcgccgattc
       61 ggcaccagcg cctccagcct gcatccgctg cagcgcctgg gcggcgcgag cctcgtcgac
      121 gcgcgcgacg gccgcgtgct cgacaccgac ggcctcgcgc gctggctggc cgcgcgcttc
      181 gagctgccat accagcgcat cgacccgctg cgcgtcgacg tcgggcgggt gtccgaggtg
      241 atgtcggtcg cctatgccga gatgcggcgc gcgctgccgc tgaacgtcgg gccgacggag
      301 gtcgtcatcg gcacgtccga gcctttcgac atcgcgtggg tgcccgagat cgaggcgcac
      361 gtgaagcggc ccgtgcggct gcagctcgcg catccggagg acatcacccg ctacaccggc
      421 gagttctatt cgctggccgc ccaggtgcgc gtcgccacgc gcgcgcagga cgccgcgaat
      481 gccgccaaca gcttcgagca gctcgtcgag ctgggcaaga acaagggctc gctcgatgcc
      541 aacgaccagg gcgtcgtgca ggtcgtcgac tggctctggc agtacgcctt cgagcagcgc
      601 gcgagcgaca tccacctcga gccgcggcgc gacatgggcg tgatccgcct gcgcatcgac
      661 ggcgtgctgc acaccgcgca ccaggtgccg gcggcggtga tgaacgcgat gacggcgcgc
      721 atcaagctgc tcggtcgcat ggacgtgatc gagcgccggc gcccgcagga cggccgcatc
      781 aagacgcgcc ggcccgacgg cgccggcggc atgcccggcg gcgaggtgga gatgcggctg
      841 tccacgctgc ccacggcctt cggcgagaag gtggtgatgc ggatcttcga ccccggcacg
      901 gtggacaagc ccttcgacgc gctgggcttc tcgccggccg aggcggagcg ctggcagtcg
      961 ctgatcggcc aggcgcacgg catcatcctc gtcacggggc cgaccggctc gggcaagacg
     1021 acgacgctgt actcgacgct caagaagctc gcgaccgacg aggtcaacgt gagcacggtg
     1081 gaggatccga tcgagatgat cgagccgtcc ttcaaccaga cgcaggtgca gcccgcggtg
     1141 gagctggact tcgcgagcgg cctgcgcgcg ctgatgcgcc aggatccgga catcatcatg
     1201 gtcggcgagg tgcgcgacct cgagaccgcg gagatggcga tccaggccgc cctgacgggc
     1261 cacctcgtct tctcgacgct gcacacgaac gacgcgccat cctcgatcac ccgcctggtc
     1321 gacctcggcg tgccgccgta cctgatctcg gccacggtga tcggcgtgct cgcacagcgc
     1381 ctcgcgcgca cgctctgcgc gctgtgcaag gcgcccgacg acaacgccga caccgtgcgc
     1441 atgctggagg aactcgcggc gccctggcgc ctgggcgggc gcatccggcc gatgaaggcg
     1501 gtcgggtgcc ccgcgtgccg gcgcaccggc taccgcggcc gcgcggccct gtacgagctg
     1561 atgccggtga ccgagccgct caaggagacg atccatcctg cgctggacgt accccggctg
     1621 acgcgccagg cgatggtcga gggcatgcgc ccgctgcggc tggccggcct gatgaaggtg
     1681 gcagagggcc tgacgacggt cgacgagatc ctgcgctgca cgccgcagtg gacggcggcg
     1741 cgggcggctc cggcgtcctg aacgacggcg gggaccgtcc ccggatttgg ggaagacgcc
     1801 gccggctgcg cggccataga ctcgcatcca acgcctcccg cgtccgcggc ggggccgtcc
     1861 gacaaccagc cgagggtctt ccgatgaaag ttcgcgtgct cgtggcggcc tgcgtggccg
     1921 cgctgtccgc cggcgccaac gccgcggtcg tcctgtccga caacttcgac gccgacgcgc
     1981 aggggctcaa cgtcgtgccg gcgggctgga ccgtcgccaa cgccggcacc gtcgacgtca
     2041 tcggcaccgg cttcttcgac gagatcccgg gcaacggcaa ctacatcgac ctcgacggct
     2101 ccaacggcca ggccgggctg ctcacgacga gcacgggcct gctgaacggc acctacacgg
     2161 cgaccttcca gctcggtggc aaccaccgcg acggcacgac cgacctggtc accgtgcagt
     2221 tcggcggcgc cagcgcggtg ttcagcgtcg gccccaacga cgccttcacc acctacagcc
     2281 tgacgaccac ggccaacctg gagcagctga gcctcagctt cctggacggc cgcggcggca
     2341 acgtcggcgc actgctggac aacgtgtcga tctcgtcggc tgtccccgag cccgcgggcc
     2401 tgccgctgct ggccgcgggc gtgctgggcg tggccttcgc cgcgcgccgc cgccgcgcct
     2461 gagccgccgc cttcctcgag ggacgcgcgc gcgccgcgcg gtggtctcgc cggcggtccg
     2521 cgacgcctgc gccgacgcga tcgacgctcc gcacgaaagc gcgattgccc acgcgcgatg
     2581 ccgcggcgcg aggcctcgcg taagctcgcg ggcgatgtcc accgactcga tcccgccgtc
     2641 gcccacgctc gcgacggtcg cgaccaccac cccggtcacc cgccccgccc gactctggcc
     2701 cggcctcgcg ctcgcgacgg cgggctccat cgccttctcg ggcaaggcga tcatcgtcaa
     2761 gctcgcgtac cgccacggcg tcgacgcggt gacgctgatc atgtaccgga tgctcttcgc
     2821 gctgccgctg ttcgcggcgc tcgcctggtg ggcgggacgc gggcggccgg cgctgaccgg
     2881 gcgcgactgg cgcgcgatcg ccgggctcgg cctgacgggc tactacctcg cgagcttcct
     2941 ggacttcgcc ggcctcgcct acgtcagcgc ggggctggag cgactggtgc tctacctgaa
     3001 cccgacgctg gtgctgctgc tcggacgggt gctgttcaag cgccgggtcg ctgcgcgcca
     3061 gcttggcgcg ctggcggtga gctatgccgg cgtgctgctg gtcttcggcc acgaggcgca
     3121 cctggccggc ggccacgtcg tcctcggcac gctgctcgtc ttcgcgagcg cggtgagcta
     3181 cgccgtctac atgaccttca gcggcgagga ggtcaggcgc ctgggctcgc tgcggctcac
     3241 cggcctcgcg tccaccgtcg cctgcgcgtt gtgcatcggc cagttcctgc tgctgcggcc
     3301 gctcgcggca gcgcgggtgg acgacgcggt gatcgggctg tcggtgctca acgcggtcgc
     3361 gtgcaccgtc gcgcccgtgc tgatggtcat gatggccatc gagcgcgtcg gcgccacggt
     3421 ggccgcgcag accggcatgg tcggccccct gagcaccatc ctcatgggcg tcgccgtcct
     3481 cggcgagccc ttcaccgcct ggatcgcggc cggtaccgcg tgcgtgctgt cggggatctg
     3541 gctgctggcg agggcgcggc cggcgacctg acgacccggc gagccgacct agcgcttgcg
     3601 agcctgtccc ggccgcacga gcccgcggcg ctcgatcggc gtcgagaaga cgatcgaggt
     3661 gcgcgtctcg ccatagccgt tgatggtgcc caggaagcgc tcgaggtcgg gcaggtcgcg
     3721 cgcgacgacg gtgaacaggt aggagtcggc gccggcgaca tggtggcact cgagcacctc
     3781 ggccgagcgg cgcagcttgt cgagcaacgc cttcttgccc ggctgcagga cggtgatgcc
     3841 gacgatggcc cgcaccggcc agcccgcgag cgccgcgtcg atctcggcgc gcgtgccgcg
     3901 cagcacgccg gcgtcctgca ggcgcttcag gcgctcggcg gtcgccgcga tggacagtcc
     3961 cgccgccgcg gcgagcgtct tcagcgacgc gcgggcgtcg gcctgcagcg cgtcgatcag
     4021 cttccacgcc ttgtcgtcga cgatgcgctc cgcgagggct ccgggcattc ggtctcctgg
     4081 ccggaaatat tccggcggtc gccgggttct accagaaatc cgcactgtgc cggcaatcgc
     4141 gcggatcgca gaatcaacgg catgggcttg tcgaccatcc tccagggcgc gggcatcggc
     4201 ttcgcgatcg cggcgccggt ggggccgatc ggactgctgt gcatccggcg caccttgcgc
     4261 gacggaccgg cggccggcct cgccaccggc ttcggcgcgg cgaccgccga cggcgtgtac
     4321 ggcctcgtcg cggccggcgg gctgggcgtc gcgtcctggc tgctcggg
//
LOCUS       DASVLN010000164         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_8801, whole genome shotgun sequence.
ACCESSION   DASVLN010000164 DASVLN010000000
VERSION     DASVLN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8801"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VF453_03775"
     CDS             complement(<1..823)
                     /locus_tag="VF453_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7686798.1"
                     /translation="MRTPIDAFAHAAAGAAGALEWPFFDAAHRQRQAALARWLGDLAS
                     DLPAPLDPQAPQDELDTHCRTWVRRLGEAGWLRVAVGGVAHGGESETLDTRTLCLMRE
                     TLAAHDGLADFAFAMQGLGSGAITLAGTPALKARYLPRVARGEAIAAFALSEPDAGSD
                     VAAMACTARRDGGTWVLDGEKTWISNGGIADFHVVFARVVEPGAAPEARGTKTIAAFV
                     VDADAPGLRIAERIATMAPHPLARLAFEGCRVPAAHCLAEGGEGFKLAMRTLDVFR"
     gene            complement(825..1682)
                     /locus_tag="VF453_03780"
     CDS             complement(825..1682)
                     /locus_tag="VF453_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX7686799.1"
                     /translation="MTDRKPLDSALAAGNRRALAGYQATHFRWECVDRVARITLDRPE
                     RKNPLSFESYAELRDLFAALRHAADVRAVVIEGAGGNFCSGGDVHEIIGPLIGLPAPE
                     LLMFTRMTGDLVKAMRHCPQPIVAAVDGVCAGAGAILAMASDLRYGTARTKTAFLFAR
                     VGLAGCDMGACAMLPRIVGQGRAAEWLYSGRALGGEEAAAWGFYNRLVEPDALRDEAT
                     AFARQLADGPAFAHALTKTMLHQEWSMDIDTAIESEAQAQALCMLTEDFGHAYRAFAA
                     KSRPAFEGR"
     gene            complement(1709..2269)
                     /locus_tag="VF453_03785"
     CDS             complement(1709..2269)
                     /locus_tag="VF453_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7686800.1"
                     /translation="MRSPARDPRSTDRLDELIGDDAIGHEGRAGSADHAAVRTWLRLL
                     SCATQVEAEIRRRLRVQFDMTLPRFDYLAQLYRFRDDQPQGLRMSALSKYLMVTGGNV
                     TSLTDDLEKSGHVTRRVDPDDRRSFRIALTTRGRRAFERVAQAHERWIVELFSGLDDQ
                     EGAQLHALLGHLRLSVTRRLHAQDER"
     gene            complement(2281..3039)
                     /locus_tag="VF453_03790"
     CDS             complement(2281..3039)
                     /locus_tag="VF453_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7686801.1"
                     /translation="MLVTGATRGIGAAIAQAMAAAGWHVTLAGRQLGALEIVRATLAV
                     GEGVAHDCLVLDVTDPASVEAAFAQAHAKGGALQALVNNAGAVETAPLAKTPLASWER
                     MLAVNLTGAFLCTQAALPPMLAAGAGRIVNIASTAGQKGYAYCTAYAAAKHGVIGMTR
                     SLALEVAARGIAVNAVCPGYTDTDIVRDGVARLVAATGRDEATARATFSQASPLQRLV
                     EPAEVAATVRWLCADAPAALTGQALSISGGEVMN"
BASE COUNT          389 a         1102 c         1157 g          402 t
ORIGIN      
        1 accggaacac gtcgagcgtg cgcatcgcga gcttgaagcc ctcgccgccc tcggccaggc
       61 agtgggcggc cggcacgcgg cagccctcga acgcgagccg cgcgagcgga tgcggggcca
      121 tcgtggcgat gcgctcggcg atgcgcaggc cgggcgcgtc ggcgtcgacg acgaaggcgg
      181 cgatcgtctt cgtcccgcgc gcctcgggcg ccgcaccggg ctcgacgacg cgcgcgaaga
      241 cgacgtggaa gtcggcgatg ccgccgttgg agatccaggt cttctcgccg tccaggaccc
      301 aggtgccgcc gtcgcggcgg gcggtgcagg ccatggccgc gacgtcggag ccggcgtccg
      361 gctcggagag cgcgaacgcc gcgatcgcct cgccgcgggc gacgcgagga agatagcgcg
      421 ccttcagcgc cggcgtgccc gcgagggtga tggcgccgct gcccaggccc tgcatcgcga
      481 aggcgaagtc ggcgaggccg tcgtgggcgg ccagcgtctc gcgcatcagg cacagcgtgc
      541 gggtatcgag cgtctccgac tcgcccccgt gcgcgacgcc gccgacggcg acacgcagcc
      601 agcccgcctc gcccagccgg cgcacccagg tacggcagtg ggtatcgagc tcgtcctgcg
      661 gcgcctgcgg atccagcggc gcgggcaggt cgctcgcgag gtccccgagc cagcgcgcga
      721 gcgcggcctg gcgctggcgg tgcgcggcat cgaagaaagg ccattcgagc gcgccggccg
      781 cgccggcggc cgcgtgggcg aaggcgtcga tcggcgtcct catgtcagcg accctcgaac
      841 gcgggcctgg acttcgccgc gaacgcgcgg tacgcgtgtc cgaagtcctc ggtgagcatg
      901 cacagcgcct gcgcctgcgc ctcggattcg atcgcggtgt cgatgtccat gctccactcc
      961 tggtgcagca tcgtcttggt cagcgcgtgc gcgaaggccg ggccgtcggc gagctggcgc
     1021 gcgaacgccg tggcctcgtc ccgcagcgcg tcgggctcga cgagccggtt gtagaaaccc
     1081 caggccgcgg cctcctcgcc gccgagcgcg cggccggaat acagccactc cgccgcgcgg
     1141 ccctggccga cgatgcgcgg cagcatcgcg caggcgccca tgtcgcagcc ggcgaggccc
     1201 acccgggcga acaggaacgc cgtcttcgtg cgcgccgtgc cgtagcgcag gtcgctcgcc
     1261 atcgcgagga tcgcgcccgc gcccgcgcac acaccgtcga ccgccgcgac gatgggttgc
     1321 gggcagtggc gcatcgcctt cacgaggtcg cccgtcatgc gcgtgaacat cagcagctcg
     1381 ggcgccggca gtccgatcag cggcccgatg atctcgtgga catcgccgcc cgagcagaag
     1441 ttgccgcccg cgccctcgat caccaccgcg cgcacgtcgg ccgcgtggcg cagcgcggcg
     1501 aacaggtcgc gcagctcggc gtagctctcg aagctcagcg ggttcttgcg ctcggggcgg
     1561 tccagcgtga tgcgggcgac gcggtcgacg cattcccagc ggaagtgcgt ggcctggtag
     1621 ccggccagcg cgcggcggtt gccggcggcg agcgcggagt cgagaggctt gcggtcggtc
     1681 atggtcggta cgtgttgggg tatggatgtc agcgctcgtc ctgcgcgtgc agccggcgcg
     1741 tgaccgacag ccgcaggtgg ccgagcaggg cgtgcaactg cgcgccttcc tggtcgtcga
     1801 ggccggagaa cagctcgacg atccagcgtt cgtgcgcctg ggcgacgcgc tcgaacgcgc
     1861 gccggccgcg cgtcgtcagc gcgatgcgga acgagcgccg gtcgtcgggg tcgacccggc
     1921 gcgtgacgtg gccgctcttc tcaaggtcgt cggtcaggct ggtcacgttg ccgccggtca
     1981 ccatcaggta cttcgacagc gcgctcatgc gcaggccctg cggctggtcg tcgcggaagc
     2041 ggtacagctg cgcgaggtag tcgaagcgcg gcagcgtcat gtcgaactgc acgcgcaggc
     2101 ggcggcggat ctcggcctcg acctgcgtcg cgcacgacag caggcgcagc caggtcctga
     2161 ccgcggcatg gtcggcggac ccggcgcgcc cctcgtggcc gatcgcgtcg tcgccgatga
     2221 gctcgtccag gcggtcggtg ctgcgcgggt cgcgggcggg gctgcgcatc gggcgtttcc
     2281 tcaattcatc acctcgccgc cgctgatcga cagggcctgg cccgtcaggg cggcgggcgc
     2341 gtcggcgcag agccagcgca ccgtcgccgc gacctccgcg ggttcgacca gccgctgcaa
     2401 cgggctcgcc tgggagaagg tcgcgcgcgc ggtggcctcg tcgcgaccgg tcgcggcgac
     2461 cagccgggcg acgccgtcgc gcacgatgtc ggtgtcggtg tagccggggc acacggcgtt
     2521 gacggcgatg ccgcgtgcgg ccacctccag cgccagcgag cgcgtcatgc cgatgacgcc
     2581 gtgcttggcc gcggcatagg cggtgcagta ggcatagcct ttctggcccg cggtgctcgc
     2641 gatgttgacg atgcggcccg cgcccgcggc cagcatcggc ggcagcgcgg cctgcgtgca
     2701 caggaacgcg ccggtcaggt tgacggcgag catgcgctcc cagctcgcga gcggcgtctt
     2761 cgcgagcggc gccgtctcga ccgcgcccgc gttgttgacg agcgcctgca acgccccgcc
     2821 cttcgcatgc gcctgcgcga acgcggcctc gacgctggcg gggtcggtga cgtcgagcac
     2881 gaggcagtcg tgcgccacgc cttcgccgac ggcgagcgtc gcgcgaacga tttcgagcgc
     2941 gccgagctgc cggcccgcca gcgtgacgtg ccatcccgcg gccgccatcg cctgggcgat
     3001 cgccgcgccg atgccgcgcg tcgcgccggt gacgagcacg cgcggcgcag
//
LOCUS       DASVLN010000165         2568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_438754, whole genome shotgun sequence.
ACCESSION   DASVLN010000165 DASVLN010000000
VERSION     DASVLN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438754"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1470
                     /locus_tag="VF453_03795"
     CDS             295..1470
                     /locus_tag="VF453_03795"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEX7686802.1"
                     /translation="MPKNIVFCADGTWSGDDKDPLDATAPTNVLKLFHDLAGTDTLET
                     FSLSDEQERILHDATGGVTQVAKWIHGVGDSDNWLVKVVGGSVGAGLIKHVVRGYTFV
                     SRWYEPGDRIHLVGFSRGAYTARALAGLIDARGLLDASRMDLDDREEAYRAAASQWTA
                     YRRARLRYDPLAIGKLEELLTLLPGFFDKPHEPAPLLPDVPVEVVAVWDTVGSMGIPV
                     FDGHGANIDALRFTDARLSGHVKFGIQVIAIDEQRGNFTPCLWQDDPRVTQWLFAGAH
                     TDCGGGNPAAAGESGLSDISLGWMIDRLRTHGVLFLSPLAYQPAPDPRAPAHAPWRHA
                     PWRYFPTAIRPCDRDGQFPAGLTLHPSVKERLAAAAVLPEVGGQPAPYAPANLAAYR"
     gene            1906..>2568
                     /gene="kdsA"
                     /locus_tag="VF453_03800"
     CDS             1906..>2568
                     /gene="kdsA"
                     /locus_tag="VF453_03800"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX7686803.1"
                     /translation="MTTVTLNDSIAVDNSRPFVLFGGINVLESRELALRSAEQYVRVT
                     RKLGIPYVFKGSYDKANRSSIHSYRGPGLEEGLRILEAVKSEFGVPVLTDVHEPWQAQ
                     PVSEVVDVLQLPAFLARQTDLVVALARTGRVCNIKKPQFLSPSQVKNIVEKFVEAGNP
                     NLILCDRGTCLGYDNLVVDMLGFSVMRRTTGDLPVIFDVTHALQQREAGAAASGGRRA
                     QVA"
BASE COUNT          393 a          968 c          868 g          339 t
ORIGIN      
        1 ggagcgctcg gcggggtcgc gggggtcgga tcggtcacgg gcacggtcgg catggacgga
       61 agtgtcggac tttccgggac gcgcggccat ggcctggcga ctcgttgccg cgggcgtcac
      121 gccggtgtca cgcgggcgtc tcccggacgc gacgtcgggc ggggcaccgg cccgcccgcc
      181 ggtcggccgc gcgacatgcg ggcgggttga gtccgcgcaa tccaggccgc cccgcttcgc
      241 gctcagatcg gatccgatcg cccggcgtcc gggcgagacg ggggaagctt cgccgtgcca
      301 aagaacatcg tcttctgcgc tgacggaacc tggtccggcg acgacaagga tccgctggac
      361 gccaccgcgc ccacgaacgt cctgaagctg ttccacgacc tcgcgggaac cgacaccctc
      421 gagacgttca gcctgtcgga cgagcaggag cgcatcctcc acgacgcgac cggcggcgtc
      481 acgcaggtcg cgaagtggat ccacggcgtc ggggactccg acaactggct cgtcaaggtc
      541 gtcggcggat ccgtcggggc cgggctcatc aagcatgtcg tgcgcggcta caccttcgtc
      601 tcgcgatggt acgaaccggg ggatcgcatc cacctcgtcg gcttcagccg cggcgcctac
      661 accgcccgcg ccctggcggg cctgatcgac gcccgcggcc tgctggacgc gagccgcatg
      721 gacctcgacg accgcgaaga ggcctaccgg gcggccgcat cgcagtggac cgcctatcgc
      781 cgcgcgaggc tgcgctacga cccgctggcg atcggaaagc tcgaggagct cctcacgctg
      841 ctgccgggat tcttcgacaa gccgcacgag cccgcgccgc tgctgcccga cgtgcccgtc
      901 gaagtggtcg cggtctggga cacggtcggc tcgatgggca tcccggtgtt cgacggccac
      961 ggcgcgaaca tcgacgcatt gcgcttcacc gacgcccgcc tgagcgggca cgtgaagttc
     1021 ggcatccagg tcatcgccat cgacgaacag cgcggcaact tcacgccttg cctgtggcag
     1081 gacgatccgc gtgtcacgca gtggctcttc gccggcgccc acacggactg cggcggaggc
     1141 aacccggcgg cggccggcga gtcgggcctc tcggacatca gcctcggctg gatgatcgat
     1201 cgcctgcgga cgcacggcgt cctgttcctg agcccgctcg cctaccagcc cgcgccggac
     1261 ccgcgcgcgc cagcgcacgc gccgtggcgc cacgccccct ggcgctactt cccgacggcc
     1321 atccgcccct gcgaccgcga cggccagttc ccggccggcc tgaccctgca tccctccgtg
     1381 aaggagcgcc tcgccgccgc ggcggtcctg cccgaggtcg gcgggcagcc cgcgccctac
     1441 gcgcccgcca acctggccgc ctatcgctag ggcggctcgt ccgcgatcca gcgacgccgc
     1501 tctcggcgag ccccccgcaa gcccgcgacg cggaacggcg ccgccagccc tgcacgaaaa
     1561 gaaaacgggc gcccccagcg cccaaaacct gaagccagcc gccgggggac ggctgcgcga
     1621 aaccggtggc cacgcgcgcg acgagcgcgc tcgcgccggg tctgatgaat cgacggattg
     1681 caacctgcgt gcccgatgcc gggtcgcagg cgagatgggg ctcccgcacg ccgaagtcgc
     1741 cgccgcacgc gcccttgcag cgtgccgcag cgacaagaat tgtcagccgg cacgcccgga
     1801 aatgtcggca ccgacaaatc ctgtcatcgc cgcgccgggg ccgcccgcgg aggcgatcgg
     1861 cccgcacgcg cgaaaatggc ggccagccag tcccggagcc ccgccatgac gaccgtcacc
     1921 ctgaacgatt ccatcgccgt cgacaacagc cgccccttcg tgctgttcgg cggcatcaac
     1981 gtgctcgaat cgcgcgagct cgccctgcgc agcgcggagc aatacgtgcg cgtcacgcgc
     2041 aagctcggca tcccctacgt gttcaagggc agctacgaca aggccaaccg ctcgtcgatc
     2101 cattcctacc gcggccccgg cctcgaggaa ggcctgcgca tcctcgaggc ggtgaagtcg
     2161 gaattcggcg tgccggtgct cacggacgtc cacgagccgt ggcaggcgca accggtatcc
     2221 gaggtggtcg acgtgctgca gctgcccgcc ttcctcgcgc gccagaccga cctcgtcgtc
     2281 gcgctcgcgc gcaccggccg cgtctgcaac atcaagaagc cgcagttcct gagcccgtcg
     2341 caggtgaaga acatcgtcga gaagttcgtc gaggcgggca accccaacct catcctgtgc
     2401 gaccgcggca cctgcctggg ctacgacaac ctcgtcgtcg acatgctcgg gttcagcgtg
     2461 atgcgacgga cgaccggcga cctgccggtg atcttcgacg tgacgcacgc gctgcagcag
     2521 cgcgaggccg gcgccgcggc ctcgggcggc cggcgcgcgc aggtcgcc
//
LOCUS       DASVLN010000166         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_170343, whole genome shotgun sequence.
ACCESSION   DASVLN010000166 DASVLN010000000
VERSION     DASVLN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170343"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VF453_03805"
     CDS             <1..508
                     /locus_tag="VF453_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686804.1"
                     /translation="QPPRGKGGNGNGGRNDRGPRGDKGPGQNAGGQQGKQRGGRDRDR
                     DRNRGRQEQGSPAPVREPGDPSFIPNPLQQTFDRRAIQQRERPVVATHYDDDEDGPIG
                     PIPNPLQQTYDKRGIQGNRDAFGGKRKGGGGGGSPKQPDPLKTAVGYIGADAFVKKLN
                     SRGGRGRR"
     gene            676..1098
                     /gene="ndk"
                     /locus_tag="VF453_03810"
     CDS             676..1098
                     /gene="ndk"
                     /locus_tag="VF453_03810"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7686805.1"
                     /translation="MTERTLSIIKPDAVAKNVIGQILSRFEAAGLKIVAAQMRHLSQA
                     EAEAFYAVHKARPFFGDLVKFMISGPVMVQVLEGEGAIAKNRELMGATDPKKADKGTI
                     RADFADSIDANAVHGSDAPETAAQEIAFFFPALNIHSR"
     gene            1172..2323
                     /gene="rlmN"
                     /locus_tag="VF453_03815"
     CDS             1172..2323
                     /gene="rlmN"
                     /locus_tag="VF453_03815"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7686806.1"
                     /translation="MTTPDNLLDLDQAALKAYCERVGEKPFRATQLFRWIHQRGVGDF
                     DAMTDLARSLRAKLAGSAEIRPLQPITEQVSADGTRKWLFDVGAGNAIETVYIPEDDR
                     ATLCISSQAGCAVGCRFCSTGHQGFSRNLTTGEIIAQLWHAEHRMRAELGLDAGERAI
                     SNVVMMGMGEPLQNYDRLVPALRMMLDDHGYGLSRRRVTVSTSGVVPMIDRLREDCPV
                     ALAVSLHAPDDALRDQLVPINRKYPIDELLSACERYLDAAPRDFITFEYCMLDGVNDS
                     VAQAKALVRLVKDRLPVKLNLIPFNPFPESGLTCSPRQRVQEFGAVLIDAGIVTTIRK
                     TRGDDIDAACGQLAGEVQDRTNAQRRMTRAIPIVPEAAPPQPASPSLKH"
     gene            2338..3192
                     /gene="pilW"
                     /locus_tag="VF453_03820"
     CDS             2338..3192
                     /gene="pilW"
                     /locus_tag="VF453_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEX7686807.1"
                     /translation="MQLLSGPVRSTRWLGAAFAAACLLAGLGGCVHEGPPLPKTPRGA
                     SADGGDIATASDQTQADRLAATRLSLASAYFFDRKQPRDALDEVKLALAARPNYPDAY
                     DLRGLIYAQLNDPAIAEQSFQRARELAPHEGGWMHDYAWFLCGQKRYPEADAEFTRAM
                     AEPGYTGQARTLLAQGVCEARAGHMADAERTLLKAYEQDPANPVTAYNLADVLYVNGQ
                     YDRARFYIRRINSKDELVSAQSLWLAARIEHKSGQADQVRELGAQLHKRFPQSAEAAA
                     FDRGRFDE"
BASE COUNT          513 a         1205 c         1074 g          404 t
ORIGIN      
        1 ccagccgccg cggggcaagg gcggcaacgg caacggcggc cgcaacgacc gcggtccgcg
       61 cggcgacaag ggcccgggcc agaatgccgg cggccagcag ggcaagcagc gtggtggtcg
      121 cgaccgcgac cgcgaccgca accgcggccg ccaggagcag ggcagccccg cgccggtccg
      181 cgagccgggc gacccgagct tcatcccgaa cccgctgcag cagaccttcg accgccgcgc
      241 catccagcag cgcgagcgcc cggtggtcgc gacgcactac gacgacgacg aggacggccc
      301 gatcggtccg atccccaacc cgctgcagca gacctacgac aagcgcggca tccagggcaa
      361 ccgcgacgcc ttcggcggca agcgcaaggg cggcgggggc ggcggctcgc ccaagcagcc
      421 cgatccgctg aagaccgcgg tcggctacat cggggccgac gccttcgtga agaagctcaa
      481 cagccgggga gggcgcggcc gccgttgaac ttcccgccgc ggcgcggcct cgaagactcg
      541 tcgcgcccgc cgccgcatcg cccgccggca gcgcgtgcca agaccttttc ggcacgccag
      601 cccatccgca acgacatccg ctcggtctaa aatgccgggc tttgcttcaa gtcacctgcc
      661 tcaggagaac caagaatgac cgaacgtacc ctgtccatca tcaagccgga cgccgtcgcc
      721 aagaacgtga tcggccagat cctgtcgcgc ttcgaggccg ccggcctgaa gatcgtcgcc
      781 gcgcagatgc gccacctgtc gcaggccgag gccgaggcgt tctacgccgt gcacaaggcc
      841 cgtcccttct tcggcgacct ggtcaagttc atgatctccg gcccggtgat ggtgcaggtg
      901 ctcgagggtg aaggcgcgat cgccaagaac cgcgagctga tgggcgccac cgacccgaag
      961 aaggccgaca agggcacgat ccgcgccgac ttcgccgact cgatcgacgc caatgccgtc
     1021 cacggctcgg acgcccccga gaccgcggcc caggagatcg cgttcttctt ccccgccctc
     1081 aacatccact cgcgctgacc cggcgcgcgg cgcgcgcgga cgcggcccct tcccggcggc
     1141 cgcctcgcgc gggcccatgg actctcgcgc gatgacgact cccgacaacc tgctcgacct
     1201 cgaccaagcc gccctcaagg cgtattgcga gcgcgtgggt gagaagccgt ttcgcgcgac
     1261 ccagttgttc cgctggatcc accagcgcgg cgtcggcgat ttcgacgcca tgaccgacct
     1321 ggcgcgctcg ttgcgcgcca agctggcggg cagcgccgag atccggccgc tgcagccgat
     1381 caccgagcag gtctcggccg acggcacgcg caagtggctg ttcgacgtcg gcgccggcaa
     1441 cgcgatcgag acggtctaca tccccgagga cgaccgcgcc acgctgtgca tctcctcgca
     1501 ggccgggtgc gcggtcggct gccgcttctg ctcgaccggc caccagggct tcagccgcaa
     1561 cctgaccacg ggcgagatca tcgcccagct ctggcacgcc gagcaccgca tgcgcgccga
     1621 gctcggcctg gacgccggcg agcgcgccat cagcaacgtc gtgatgatgg gcatgggcga
     1681 gccgctgcag aactacgatc gactggtgcc ggcgctgcgc atgatgctgg acgaccatgg
     1741 ctacggcctg tcgcgccggc gcgtgaccgt ctccacgtcg ggcgtcgtcc cgatgatcga
     1801 tcgcctgcgc gaggactgcc cggtcgcgct ggccgtctcg ctgcacgcgc ccgacgacgc
     1861 gctgcgcgac cagctcgtgc cgatcaaccg caagtacccg atcgacgagc tgctgtcggc
     1921 ctgcgagcgc tacctcgacg ccgccccgcg cgacttcatc accttcgagt actgcatgct
     1981 cgacggcgtg aacgactcgg tcgcgcaggc caaggcgctg gtgcggctcg tgaaggaccg
     2041 gctgccggtc aagctcaacc tcatcccgtt caacccgttc ccggagtcgg gcctgacgtg
     2101 ctcgccgcgc cagcgcgtgc aggagttcgg cgcggtgctc atcgacgccg gcatcgtcac
     2161 gacgatccgc aagacccgcg gcgacgacat cgacgccgcc tgcggccagc tcgcgggcga
     2221 ggtgcaggat cgtaccaacg cgcagcgccg catgacgcgc gccatcccca tcgtgccgga
     2281 ggcggcgccg ccgcagccgg cgtccccgtc cctcaaacac tgatcgccag aaagcgcatg
     2341 caactcctct ccgggccggt tcgatcgacg cgttggttgg gagcggcgtt cgccgcggcg
     2401 tgcctgcttg ccgggctggg cggctgcgtg cacgagggcc cgccgctgcc gaagacgccg
     2461 cgcggcgcga gcgcggacgg cggggacatc gccaccgcgt ccgaccagac ccaggccgac
     2521 cgcctcgcgg cgacgcggct gagcctcgcg tcggcctact tcttcgaccg caagcagccg
     2581 cgcgacgcgc tcgacgaggt caagctcgcg ctggccgcgc gacccaacta ccccgacgcc
     2641 tacgacctgc gcgggctcat ctacgcgcag ctcaacgacc cggcgatcgc cgagcagagt
     2701 ttccagcgcg cgcgcgagct cgcgccgcac gagggcggct ggatgcacga ctacgcgtgg
     2761 ttcctgtgcg gccagaagcg ctatcccgag gccgacgccg agttcacgcg cgcgatggcc
     2821 gagcccggct acacgggcca ggcgcgcacg ctgctcgcgc agggcgtctg cgaggcgcgc
     2881 gcgggccaca tggccgatgc ggagcgcacg ctgctcaagg cctacgagca ggatccggcc
     2941 aacccggtga ccgcctacaa cctggccgac gtgctgtacg tgaacggcca gtacgaccgc
     3001 gcccggttct acatccggcg catcaattcg aaggacgagc tggtgagcgc gcagtcgctc
     3061 tggctggccg cgcgcatcga gcacaagagc ggccaggccg accaggtccg cgagctcggc
     3121 gcccagctgc acaagcgctt cccgcagtcg gcggaggccg cggcgttcga ccggggtcgc
     3181 ttcgatgagt gacgac
//
LOCUS       DASVLN010000167         3551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_492214, whole genome shotgun sequence.
ACCESSION   DASVLN010000167 DASVLN010000000
VERSION     DASVLN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492214"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF453_03825"
     CDS             complement(<1..228)
                     /locus_tag="VF453_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX7686808.1"
                     /translation="MTQASLFDADDLPAPPPAGLPEGLAYEPGFLSPDEEAALVALVG
                     TLPLEAAKYKAYTARRRVASFGGAFDYDANRL"
     gene            complement(266..727)
                     /locus_tag="VF453_03830"
     CDS             complement(266..727)
                     /locus_tag="VF453_03830"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HEX7686809.1"
                     /translation="MRHHPPARAVSAPPDPGCAMLLPGHQFADAYRVPVPPGMDAMAA
                     TRLAFARGPWWVRGLMALRNGVVGVFGLKAASSAGFPVISQSPERVVLGFDDRHLDFR
                     IVVALAGGCATLTTAVRWHNAFGRAYLAVVMPFHRVIAARMLEGVAGVPAA"
     gene            815..2023
                     /locus_tag="VF453_03835"
     CDS             815..2023
                     /locus_tag="VF453_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7686810.1"
                     /translation="MNLQPLDAWLWTLASMAGAAIVATLAYVVARPIVRRVTRHATVA
                     SAIVGRLDRPLRWLLPLLAMQLAIEGAPDDLAHVEGVRQLLSVLLIAAFTWCAVAAVR
                     GLVDAVIVLHPQDVADNLEARRVITQARVLSRIAIAIALFAGIAFILMTFPRARQFGT
                     SLLASAGLSALVIGLAAKSVFGNLLAGLQIALSQPIRIDDVLIVQGEWGRVEEITSTY
                     VVLKIWDERRLVIPLQWFIDNPFQNWTRTTSQIIGSVFFWVDYSTDLAPLRAEATRLA
                     QASKDYDGRVCLLQVVDTSERAMKLRLIVSSPSAGQSWDLCCLVREGIVALMRKEQPH
                     ALPRLRAEIHRTWDEPAAPATAAPSPHVEARLAGERTDTGGAAVNNDTGMSDLAAPTG
                     GVVPPQEAPR"
     gene            2304..3401
                     /locus_tag="VF453_03840"
     CDS             2304..3401
                     /locus_tag="VF453_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7686811.1"
                     /translation="MLAAAPVVASGPLFAKDSGNLVLGQSAPTTGPSAQLGIQLNRGA
                     RLYFDKVNAQGGVNGQKIELRLLDDGYEPPRAKANTQKLIDDDVFALFGYVGTPTSLA
                     CLPLVRDSGIPFFAPFTGAMALRQPVLRNVFHVRASYDDETALIVNQLHNLGLNKVAV
                     FRQNDSYGQSGLDGVVKALSRYSLAPTAVATFERNSLQVTPAVKAIVAARPDVVVQIG
                     SYKSCAAFIREARKAGYGGQFYNVSFVGTKALSDELDKEALGVVVSQVMPYPFSPAVP
                     IVRDFLDAVKAAGGDAQANYSSLEGYVDARVFVEGLRRGKSNTREGLLSGLESLQHVD
                     FDGFNLNFGRDHAASHFVELSMLTGDGGVRC"
BASE COUNT          510 a         1312 c         1221 g          508 t
ORIGIN      
        1 caggcggttg gcgtcgtagt cgaaggcgcc gccgaagctc gcgacacggc ggcgcgcggt
       61 gtacgccttg tatttcgcgg cctccagcgg cagcgtgccg accagcgcga ccagcgcggc
      121 ctcctcgtcg ggcgacagga agccgggctc gtaggcgagt ccctcgggca ggccggcggg
      181 cggcggcgcg ggcaggtcgt cggcgtcgaa gagcgaggct tgggtcatgg cacgaacgac
      241 gcaggtgcgg cgtccggtgc gcctttcaag ccgccgggac gccggcgacg ccctcgagca
      301 tgcgcgccgc gatcacgcgg tggaacggca tcacgacggc caggtaggcg cgaccgaacg
      361 cgttgtgcca gcgcacggcc gtcgtgagcg tcgcgcagcc gccggccagc gcgacgacga
      421 tgcggaagtc gagatggcgg tcgtcgaagc cgaggacgac gcgctcgggc gactggctga
      481 tcaccgggaa tcccgcggag ctcgcggcct tcagcccgaa cacgccgacc acgccgttgc
      541 gcagggccat cagcccgcgc acccaccacg gcccgcgcgc gaacgccagc cgcgtcgcgg
      601 ccatcgcgtc catgcccggc gggacgggca cgcggtaggc atcggcgaac tggtggcccg
      661 gcagcagcat ggcgcagccg gggtcgggag gcgcggagac ggcgcgggcc ggggggtggt
      721 ggcgcatggc ggaactatag tgcgcgcctg cgtggaggcg cgtcgtttgc cgggccgcgc
      781 ggtcgcctcc gtcccgtcaa cacccctgcc caccatgaac ctgcaaccgc tcgacgcctg
      841 gctctggacg ctcgcctcga tggcgggcgc cgcgatcgtg gccaccctcg cgtacgtcgt
      901 cgcgcgcccg atcgtgcgcc gcgtgacccg gcacgcgacc gtggcgtccg cgatcgtcgg
      961 ccggctcgat cgcccgctgc gctggctgct gccgctgctg gcgatgcaac tggccatcga
     1021 aggcgccccc gacgacctcg cccatgtcga gggcgtgcgg cagctgctgt ccgtgctgct
     1081 gatcgccgcg ttcacgtggt gcgccgtcgc cgcggtgcgc gggctcgtcg acgccgtgat
     1141 cgtgctgcac ccgcaggacg tcgcggacaa cctcgaggcg cgccgggtca tcacgcaggc
     1201 gcgcgtgctc tcgcgcatcg cgatcgcgat cgccctgttc gcgggcatcg cgttcatcct
     1261 gatgacgttc ccgcgcgcgc ggcagttcgg caccagcctg ctcgcctcgg ccgggctgtc
     1321 cgcgctggtg atcggcctgg cggcgaagtc ggtcttcggc aacctgctcg ccgggctgca
     1381 gatcgcgctg tcgcagccga tccgcatcga cgacgtgctg atcgtgcagg gcgaatgggg
     1441 ccgcgtcgag gagatcacca gcacctatgt cgtgctcaag atctgggacg agcgccgcct
     1501 cgtcatcccg ctgcagtggt tcatcgacaa cccgttccag aactggacgc gcaccacctc
     1561 gcagatcatc ggcagcgtgt tcttctgggt cgactactcc accgacctcg ccccgctgcg
     1621 cgccgaggcg acgcggctcg cgcaagcctc gaaggactac gacggccgcg tctgcctgct
     1681 gcaggtggtc gacaccagcg agcgcgcgat gaagctgcgc ctgatcgtga gctcgccgtc
     1741 ggccggccag agctgggacc tgtgctgcct cgtgcgcgag ggaatcgtcg ccctcatgcg
     1801 caaggagcag ccgcacgcgc tgccgcgctt gcgcgccgag atccaccgca cctgggacga
     1861 gccggccgcg cctgcgacgg ccgcgccctc gccccacgtc gaggcgcggc tcgcgggcga
     1921 gcgcacggac accggaggcg ccgcggtcaa caacgacacc ggcatgagcg acctcgccgc
     1981 gcccaccggt ggcgtcgtgc cgccgcagga agcgccacgc tgaggccgcc ccgcgccgga
     2041 accggccccg atgctgccga gaaggtgact cggcagcagg ggtaacctgt tgtaagcgaa
     2101 gtttccttac gaaaacaacg gccgagttgt caatccggac tcgtcggctc gtccgtgatg
     2161 acgatcacga aacgaccgat tgaggggctt cttttgccca tatccggagc cgaggacatc
     2221 gttgtcgggt gacaatccga tacccctccc cggttccacc gtcaaatcgc gccctcgata
     2281 tgaaacgccg ttccctgctc ggcatgctcg ccgctgcgcc ggtcgtcgcg tccggccccc
     2341 tgttcgccaa ggactcgggc aacctggtcc tcggccaatc cgcgcccacc acggggccgt
     2401 cggcgcagct gggcatccag ctcaaccgtg gcgcccggct ctacttcgac aaggtcaacg
     2461 cccagggcgg cgtcaacggc cagaagatcg agctgcgcct gctcgacgac ggctacgagc
     2521 cgccgcgcgc caaggccaac acgcagaagc tgatcgacga cgacgtcttc gccctgttcg
     2581 gctacgtcgg cacgcccacg tcgctcgcct gcctgcccct ggtgcgcgac tcgggcatcc
     2641 cgttctttgc ccccttcacg ggcgcgatgg cgctgcgcca accggtgctg cgcaacgtct
     2701 tccatgtccg cgcctcctac gacgacgaga ccgcgctgat cgtcaaccag ctgcacaacc
     2761 tcggcctgaa caaggtcgcg gtgttccgcc agaacgacag ctacgggcag tccggcctcg
     2821 acggcgtcgt caaggcgctg tcgcgctaca gcctcgcgcc gaccgcggtg gccaccttcg
     2881 agcgcaactc cctgcaggtg acgcccgcgg tcaaggcgat cgtcgcggcg cggcccgacg
     2941 tcgtcgtgca gatcggctcc tacaagagct gcgccgcgtt catccgcgaa gcgcgcaagg
     3001 ccggctacgg cggtcagttc tacaacgtct ccttcgtcgg caccaaggcg ctgtccgacg
     3061 agctggacaa ggaggccctg ggcgtcgtcg tgagccaggt catgccctac ccgttctcgc
     3121 cggccgtgcc gatcgtgcgc gacttcctgg acgccgtgaa ggccgccggc ggcgacgcgc
     3181 aggccaacta ctcgagcctc gagggctacg tcgacgcgcg cgtgttcgtc gagggcctgc
     3241 gccgcggcaa gtccaacacc cgcgagggcc tgctgtcggg cctggagtcg ctgcagcacg
     3301 tggacttcga cggcttcaac ctcaacttcg gccgcgacca cgcggcgtcg cacttcgtcg
     3361 agctgtcgat gctcacgggc gacggcggcg tgcgctgctg aggacccgcc ccgcggcgag
     3421 gtgccgaccg cggtggcgcg gccgtcgccg aagccgcgag gcgaggcctc aactcctgag
     3481 gatccgccac gcgcgcccca tcaggtcgcg gctcgatcgc gcccgcgccc ccagccgcgc
     3541 gtcgccgccg c
//
LOCUS       DASVLN010000168         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_277696, whole genome shotgun sequence.
ACCESSION   DASVLN010000168 DASVLN010000000
VERSION     DASVLN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277696"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..507)
                     /locus_tag="VF453_03845"
     CDS             complement(91..507)
                     /locus_tag="VF453_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7686812.1"
                     /translation="MNLPVPTPAQLPDRRIPLCASGELVEAGGAVEFDVLLWRRPARA
                     FALRFEGRVRAYLNRCAHVPTEMQARPGEFLDADRRFIVCAMHGAVYEPTTGRCIAGP
                     CRGEWLAAIPVVEEDGRVYWYPSPDIAPAAPDEPSA"
     gene            complement(504..1172)
                     /locus_tag="VF453_03850"
     CDS             complement(504..1172)
                     /locus_tag="VF453_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7686813.1"
                     /translation="MTDLRPRRFDLVVFDWDGTLFDSTALIVQSIQAACRDIGEPEPD
                     ARRAAWVIGLSLRAALEHAVPTLTAQRYPLLVDRYRHHYAARQHALTLFPGVLEMLQA
                     LKARHHWLAVATGKSRVGLDDALGTAALRGVFDGSRTADETRSKPDPQMLHELMRQFG
                     AEPHRTLMIGDTTHDLQLAANAGAASVAVSYGAHEPESFATHSPLLVAHSVAELAAWL
                     ERHA"
     gene            complement(1169..2197)
                     /locus_tag="VF453_03855"
     CDS             complement(1169..2197)
                     /locus_tag="VF453_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7686814.1"
                     /translation="MIEAKRRPAQTTPPAAPAAPAVRQVEVDAEFAGQRLDNFLLREL
                     KGVPKTHVYRIIRSGEVRVNKGRAGADTKLVAGDIVRVPPLRLPATSADAPAEAAPPR
                     EFPILFEDEHLVAIDKPAGTAVHGGSGVSFGVIEQLRRARPQQRYLELVHRLDRETSG
                     ILLLAKKRSALVALQEQFRARETGKTYAALVVGAWPASLKVIDVALLRTEDAAGDRRV
                     RAVSADHADGQRAISLVKVARRFADTTLLDVTIKTGRTHQIRVHLAHAGHVIVGDDKY
                     GDFALNRRYARGEAVAGVRFERMFLHARRLAFDHPATGERLELAAPLPPECARLVDAL
                     PAAMPDTP"
     gene            2973..>3695
                     /locus_tag="VF453_03860"
     CDS             2973..>3695
                     /locus_tag="VF453_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX7686815.1"
                     /translation="MKRMLINATQPEERRLAIVDGQKLLDFENEIEGREQRKGNIYKA
                     VVTRVEPSLEACFVDYGEDRHGFLPFKEISRQYFREGVDVKSARIQDVIREGQELLVQ
                     VEKEERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEDREELKENLDQLQYP
                     KGMSLIARTAGIGRSAPELQWDLNYMLKLWDAIDGASREGKGAFLIYQESSLVIRAIR
                     DYFTADIGEILIDTDDIYEQAHQ"
BASE COUNT          547 a         1246 c         1406 g          496 t
ORIGIN      
        1 cctcgtccgg cgtcgggtgc gggagatcgt gttggtcggt catgggacga tgcgggggcg
       61 tgatggggac gcggcgggcg gggcgcggcg tcaggcggac ggctcgtcgg gggcggcggg
      121 cgcgatgtcc ggggaaggat accaatacac ccggccgtcc tcctcgacga ccgggatggc
      181 ggcgagccac tcgccgcggc aggggccggc gatgcatcgg ccggtggtgg gctcgtagac
      241 cgcgccatgc atcgcgcaga cgatgaagcg gcggtcggcg tcgaggaact cgcccgggcg
      301 cgcctgcatc tcggtgggca cgtgcgcgca gcggttcagg taggcgcgca cgcgtccctc
      361 gaagcgcagc gcgaacgcgc gcgcgggccg gcgccagagc agcacgtcga attcgaccgc
      421 acctccggcc tcgaccagct cgccgctcgc gcacagcggg atgcggcggt ccggcagctg
      481 cgccggcgtc ggcacgggca ggttcatgcg tggcgctcga gccaggccgc gagctcggcg
      541 accgaatgcg cgaccagcag cggcgagtgg gtcgcgaacg actcgggctc gtgcgcgccg
      601 taggacaccg cgacgctggc ggcaccggcg ttggcggcca gctgcaggtc gtgcgtcgtg
      661 tcgccgatca tcagcgtgcg gtgcggctcc gcgccgaact ggcgcatcag ctcgtgcagc
      721 atctgcgggt cgggcttgga tcgcgtctcg tcggcggtgc gcgagccgtc gaacacgccg
      781 cgcagggccg cggtgccaag cgcgtcgtcc aggccgacgc ggctcttgcc ggtggccacc
      841 gcgagccagt ggtggcgcgc cttcagcgcc tgcagcatct ccagcacgcc ggggaacagc
      901 gtgagcgcat gctggcgcgc ggcgtagtgg tggcggtagc ggtcgaccag cagcgggtag
      961 cgctgcgcgg tgagcgtggg cacggcgtgc tcgagcgcgg cgcgcaggct caggccgatc
     1021 acccaggcgg cgcggcgcgc gtcgggctcg ggctcgccga tgtcgcggca ggcggcctgg
     1081 atgctctgca cgatcagggc ggtggagtcg aacagggtgc cgtcccagtc gaagacgacg
     1141 aggtcgaagc ggcgcgggcg gaggtcggtc atggcgtgtc gggcatggcg gccgggaggg
     1201 cgtcgacgag gcgggcgcac tcgggcggca gcggcgcggc gagctcgagg cgctcaccgg
     1261 tggcgggatg gtcgaacgcg agccggcggg cgtgcaggaa catgcgctcg aagcggacgc
     1321 ccgcgacggc ctcgccgcgc gcgtaacggc ggttgagggc gaagtcgccg tacttgtcgt
     1381 cgccgacgat cacatggccg gcatgggcga gatggacgcg gatctggtgc gtgcggccgg
     1441 tcttgatggt gacgtccagc agcgtcgtgt ccgcgaatcg gcgcgccacc ttcacgagcg
     1501 agatggcgcg ctggccgtcg gcatggtcgg cggacacggc gcgcacgcgg cggtcgccgg
     1561 ccgcgtcctc cgtgcgaagg agcgcgacgt cgatcacctt cagcgacgcc ggccaggcgc
     1621 cgaccacgag cgcggcgtag gtcttgccgg tctcgcgcgc gcggaactgc tcctgcaggg
     1681 ccaccagcgc gctgcgcttc ttggcgagca gcaggatgcc cgaggtctcg cgatcgagcc
     1741 ggtgcacgag ctcgaggtag cgctgctgcg ggcgcgcgcg gcgcaactgc tcgatcacgc
     1801 cgaagctcac gcccgagccg ccatggacgg cggtgccggc gggcttgtcg atggcgacca
     1861 ggtgctcgtc ctcgaacagg atcgggaact cgcgcggcgg ggcggcctcg gccggcgcgt
     1921 cggcggaggt ggcgggcagg cgcagcggcg gcacgcgcac gatgtcgcct gccacgagct
     1981 tggtgtcggc gcccgcgcgg cccttgttca cgcgcacctc gccggagcgg atgatgcgat
     2041 agacgtgcgt cttgggcacg cccttgagct cgcgcaggag gaagttgtcc aggcgctggc
     2101 ccgcgaattc ggcgtcgacc tcgacctggc ggacggccgg tgccgccggg gccgccggcg
     2161 gggtcgtttg ggccggtctg cgtttcgcct ctataattgg ccggaccacg ttggtgccat
     2221 aagttcttga ttttccagag tttacccggg tgtttcccgc gcaggctctc gaagaccgga
     2281 cgtccacggc cccggcgcgg cgccggaacc cgcaagggcg ccggtcaaca aggtgatgca
     2341 acgcgtacgc gccctctgca ggccggcgac cacgaagtgg cccggcggtc gacggcaagg
     2401 acgtggaaga gcaacggcac atgaaccgcc ctgcagaggc tttgcaggct ggagatagaa
     2461 gttttccgaa gccgccaggc gcgtcgggcc ccgtgggtcc ggcgagcgct ggcggccggc
     2521 gtcggcaacg catccgcaaa cgcatggcgt tcgttttcca cccccacccc gtcccgcacc
     2581 gccgcgcggt cgagtcccgg cccgggccgc tgccgccacg cctcgtgcgt gccgcagccg
     2641 ccgccgcgcg ctcgccgcat ggccggggcc gcgaggtgcg accccctgca agagcggcca
     2701 cggccgcgtt cgcaggcggc caggggaaga cgctgcgcat cctgccccgc ggccgcgtga
     2761 cctgacgtcc aagcgccaac gctgccacga ggtcgccgcc ccatccgggc atgaccggca
     2821 gtccagggcg attccttcga tcggttcggt ttcgtttctc gtgcatcgtt ccgcgcccca
     2881 gagcccgcga caggcggcca gggcgcaacg aggtgccgtc gcctcgaggt ggccaacggg
     2941 cccccgggac ggcggcggat gaaggagtgc acgtgaaacg catgctgatc aacgcgacgc
     3001 agccggaaga acgccggctg gccatcgtcg acggacagaa actgctcgat ttcgagaacg
     3061 agatcgaggg ccgcgaacag cgcaagggca acatctacaa ggcggtcgtg acgcgggtcg
     3121 agccctcgct cgaggcctgc ttcgtcgact acggcgagga tcgccacggc ttcctgccgt
     3181 tcaaggagat ctcgcgccag tacttccgcg agggcgtgga cgtcaagtcc gcgcgcatcc
     3241 aggacgtcat ccgcgaaggc caggagctcc tggtccaggt cgagaaggaa gaacgcggca
     3301 acaagggcgc cgcgctgacg accttcatct cgctggccgg ccgctacctc gtgctgatgc
     3361 ccaacaaccc gcgcggcggc ggcgtgagcc ggcgcatcga gggcgaggac cgcgaggagc
     3421 tcaaggagaa cctcgaccag ctgcagtatc ccaagggcat gagcctgatc gcgcgcaccg
     3481 ccggcatcgg ccgcagcgcg cccgaactgc agtgggacct caactacatg ctcaagctgt
     3541 gggacgccat cgatggcgcc tcgcgcgagg gcaagggcgc gttcctgatc taccaggagt
     3601 cgtcgctggt catccgcgcg atccgcgact acttcacggc ggacatcggc gagatcctga
     3661 tcgacaccga cgacatctac gagcaggcgc accag
//
LOCUS       DASVLN010000169         7318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_9127, whole genome shotgun sequence.
ACCESSION   DASVLN010000169 DASVLN010000000
VERSION     DASVLN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9127"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF453_03865"
     CDS             complement(<1..189)
                     /locus_tag="VF453_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686816.1"
                     /translation="MGSIDTGGLATSKARELNADAWGLLVERSRALVHHASSLMGTLT
                     HPALADDDADHAAVRAAVE"
     gene            complement(398..1069)
                     /locus_tag="VF453_03870"
     CDS             complement(398..1069)
                     /locus_tag="VF453_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7686817.1"
                     /translation="MRILIAEDDQVLADGLLRSLRGSGYAVDQVSSGTEADAALASHE
                     FDLLILDLGLPRLHGMEVLRKLRARGSSLPVLILTAADSVDQRVKGLDLGADDFMAKP
                     FSLQELEARVRALTRRGLGTASSVIRHGPLTFDANGRVAYINDQMIELSAREISLLEV
                     LLQRAGRLVSKDQLVTRLCEWGEEVSNNAIEVYIHRLRKKIEQGPIRIATVRGLGYCL
                     EKIAG"
     gene            1363..2448
                     /gene="recA"
                     /locus_tag="VF453_03875"
     CDS             1363..2448
                     /gene="recA"
                     /locus_tag="VF453_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX7686818.1"
                     /translation="MDATKNASAKNAPAPAAASALNTEKAKALQAALAQIEKQFGKGS
                     IMRLGESEKIEDIQVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVAAE
                     MQKLGGVCAFIDAEHALDIQYAQKLGVNLQELLISQPDTGEQALEIVDALVRSGSVDL
                     IVVDSVAALTPKAELEGEMGDSLPGLQARLMSQALRKLTGTIKKSNTMVIFINQIRMK
                     IGVMFGSPETTTGGNALKFYASVRLDIRRIGSIKRGEEIIGNETKVKVVKNKVSPPFK
                     EAEFDILYGAGISREGEIIDLGVAAKVVEKSGAWYAYNGEKIGQGKDNCREFLKENPA
                     LAVEIENKVRESLGVPLVPVGDDADKG"
     gene            2727..3170
                     /locus_tag="VF453_03880"
     CDS             2727..3170
                     /locus_tag="VF453_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686819.1"
                     /translation="MALTALAATTAAFVQVAGFDEPGDVAIVEALVRAIRSPEGFERL
                     MSLETFPQTVAAGAARGTGSGQADRDAPGGGATGSAAARRGAAGGAAMDARATPVAPH
                     RLADDHRASDFFVFADDHCLDRLTAASRFFCAPGGGFLATIPDWA"
     gene            3167..3874
                     /locus_tag="VF453_03885"
     CDS             3167..3874
                     /locus_tag="VF453_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEX7686820.1"
                     /translation="MTSPERSRRARARPSLKMQAIALLSRREHSRQELRAKLLAALRR
                     RAREDAAEAAASQAHADPVERALAQTPSNGIDDAFLPRPRARLPVPPPRNSVVVDSDG
                     FLRPAQEPQAPSQHEREQDADPEAVVDALLDWLAARGYLSDERFVETRVHARAAKQGA
                     ALIRQELARHGLALQPEQATALRESEFARAQALWQRKFGEVADEPRERARQARFLASR
                     GFAADVVRRIVGGQDEF"
     gene            4175..5335
                     /gene="sucC"
                     /locus_tag="VF453_03890"
     CDS             4175..5335
                     /gene="sucC"
                     /locus_tag="VF453_03890"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX7686821.1"
                     /translation="MKIHEYQGKELLRQFGVPVPRGYPAFTVQEAVEAAQKLGGSVWV
                     VKAQIHAGGRGKGGGVKLARSLDDVKALAGEILGMQLKTHQTGPEGQKVRRLYIEEGA
                     DIKKEYYVSLVTDRATQKVALIASSEGGMDIEEVAHSSPEKIITEVIDPLAGLTAEQA
                     KKVAAAVGLPEGSQAQAVDLFQKLYQCYMDTDASLVEINPLNCDSKGALIALDAKFNF
                     DANALFRHPEIVAYRDLDEEDPAEIEASKFDLAYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGGEPANFLDVGGGATAEKVTEAFKIMLKNPHVKGILVNIFGGIMKCDTIAEGVIQ
                     ACKAVNLSVPLVVRMKGTNEELGKKMLAESGLPIISADTMAEAATKIVAAVK"
     gene            5370..6263
                     /gene="sucD"
                     /locus_tag="VF453_03895"
     CDS             5370..6263
                     /gene="sucD"
                     /locus_tag="VF453_03895"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX7686822.1"
                     /translation="MSIYINKDTKVITQGITGKTGQFHTLGCQAYANGKNAFVAGVNP
                     KKAGEKFSDIPIYATVSEARQQTGANVSVIYVPPAGAAAAIWEAVEADLDLVVCITEG
                     IPVRDMLVVRNKMKAKEAAGGKKTLLLGPNCPGLITPDEIKIGIMPGHIHRKGRIGVV
                     SRSGTLTYEAVAQLTEIGLGQSSAVGIGGDPINGLKHIDVMKAFNDDPETDAVIMIGE
                     IGGPDEAEAARWCKANMKKPVVGFIAGVTAPAGKRMGHAGALISGGADTADAKLAIME
                     ECGFTVTRNPSEMGKLLKGLL"
     gene            6403..7098
                     /locus_tag="VF453_03900"
     CDS             6403..7098
                     /locus_tag="VF453_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7686823.1"
                     /translation="MDLTSPAFWTALGSIILANILLSGDNAVVIAMAARALPAHQQKR
                     AIVFGSVAAIVMRVVLTLIAVKMLTLPYLKLVGGALLLWIGVNLLNEEDDGHAHGTQA
                     PGVAAAIRTILVADLVMSLDNVLAVAAAAQGNTPLLVIGLAVSVPLIVFGSTLLLKVM
                     ERFPLIITAGAALLGWLAGEMMLGDPGIVRRFGEIPEAWVTGAGIAGAVLVVAVGLAL
                     KARRKRFQQAPGS"
BASE COUNT         1175 a         2550 c         2534 g         1059 t
ORIGIN      
        1 ctccacggcg gcgcggaccg cggcgtggtc ggcgtcgtcg tcggcgagag cgggatgcgt
       61 gagcgttccc atcagcgacg aggcatggtg gacgagagcg cggctgcgtt cgaccagcag
      121 gccccaggcg tcggcgttca attcgcgggc tttcgaagtg gcgaggccgc cagtgtcgat
      181 cgatcccatg cctgtcgagt tgcaagctcc gggcccgatg tggcgcctgc acgaaccgcg
      241 gccgccggcg ttgcgcgaaa cggcgcgcgg cgtgcgatat ctcacgcgcc gggtggcctc
      301 cggacgatcg ccgcaggcgc gcgccgcgtc gcgggcagcc gcccgaggcg ccccgcggtc
      361 ggcacgccga cggggcgcgg acgttcttgc cgcaggatca gccggcaatc ttctccaggc
      421 agtacccgag gccccgcacc gtggcgatgc ggatcggccc ctgctcgatc ttcttgcgca
      481 ggcggtggat gtagacctcg atggcgttgt tgctcacctc ctcgccccat tcgcacaggc
      541 gggtgacgag ctggtccttg ctcacgaggc ggcccgcgcg ctgcagcagc acctcgagca
      601 ggctgatctc gcgcgccgac agctcgatca tctggtcgtt gatgtaggcc acgcgcccgt
      661 tggcgtcgaa ggtgagcggc ccgtggcgga tcacgctcga ggccgtgccc agcccgcgcc
      721 gcgtgagcgc gcgcacgcgg gcctcgagtt cctgcagcga gaatggcttg gccatgaagt
      781 cgtcggcgcc caggtcgagg cccttgacgc gctggtcgac cgagtcggcc gcggtgagga
      841 tcagcaccgg cagcgacgag ccgcgcgcgc gcagcttgcg cagcacctcc atgccgtgca
      901 ggcgcggcag gccgaggtcg aggatcagca ggtcgaactc gtgcgaggcg agggcggcat
      961 cggcctcggt gccgctggag acctggtcga ccgcgtagcc ggagccgcgc agggagcgca
     1021 gcagcccgtc ggcgaggacc tggtcgtctt ccgcgatgag gatgcgcatg gcggggttgt
     1081 ctccttgccg gctccgggcg ttttcagtcg attcggcgcg cgcggccggc ctctcgcccg
     1141 ggttaatgcc cggtttcgcc gcggggatgc tcgtggggcg ccgctacatt cgttttgtcg
     1201 attctaagaa ccccgcgacc cgtccgattg cgatcgcgca gtggctcatc cagcgagctt
     1261 gtgcctcgcc acaatgaccg aaaccctgca aaagggctgt acagatatcc agccctgtcc
     1321 cataattccc ggcagcgaaa actgcccaac ccggagatcc gcatggacgc caccaagaac
     1381 gcttccgcca agaacgcccc cgcgccggcc gccgccagcg cgctgaacac ggagaaggcc
     1441 aaggcgctgc aggctgcgct cgcgcagatc gagaagcagt tcggcaaggg ctcgatcatg
     1501 cgcctgggcg agagcgaaaa gatcgaggac atccaggtcg tgtccaccgg ctcgctgggc
     1561 ctggacatcg cgctgggcgt cggcggcctg ccgcgcggcc gcgtggtcga gatctacggc
     1621 ccggagtcgt ccggcaagac cacgctgacg ctgcaggttg ctgccgagat gcagaagctc
     1681 ggcggcgtct gcgccttcat cgacgccgag cacgcgctgg acatccagta cgcgcagaag
     1741 ctcggcgtca acctgcagga gctgctgatc tcgcagcccg acaccggcga gcaggcgctc
     1801 gagatcgtgg acgcgctcgt gcgctccggc tcggtggacc tcatcgtcgt cgactccgtc
     1861 gcggcgctga cgcccaaggc cgaactcgag ggcgagatgg gcgactcgtt gcccggcctg
     1921 caggcccgcc tgatgagcca agccctgcgc aagctgaccg gcaccatcaa gaagagcaac
     1981 acgatggtga tcttcatcaa ccagatccgc atgaagatcg gcgtgatgtt cggcagcccc
     2041 gagacgacca ccggcggcaa cgcgctcaag ttctacgcct cggtccgcct ggacatccgc
     2101 cgcatcggct cgatcaagcg cggcgaggag atcatcggca acgagaccaa ggtcaaggtc
     2161 gtcaagaaca aggtctcgcc cccgttcaag gaagccgagt tcgacatcct ctacggcgcg
     2221 ggcatcagcc gcgagggcga gatcatcgac ctgggcgtcg cggccaaggt ggtcgagaag
     2281 tccggcgcct ggtacgccta caacggcgag aagatcggcc agggcaagga caactgccgc
     2341 gaattcctga aggagaaccc ggccctggcg gtcgagatcg agaacaaggt tcgcgagtcc
     2401 ctgggcgtgc cgctggtgcc ggtcggcgac gatgccgaca agggctgacg cacgcgactg
     2461 atttgcgccg tcacgcgatg gcgtcctccg ccgcgctcct gccgccgcct ctcgccacga
     2521 cgaggcgaac gtccgtgagt gggccttcca cgaatcctgc gcggagcaag cgggccggcc
     2581 cgctgtcgcc tcgcgcactg cctcggagcg tgtcgatgag cgctggcgcc ctgcgtcccg
     2641 tcgaacggcc cggacggcaa gttcgtccgg ggcgtccgcg tcgtcccggt catccggctg
     2701 gaaggccggc gttcgtggag cggcggatgg cgttgacggc gctggccgcc acgacggcag
     2761 cgttcgtcca ggtcgccgga ttcgacgaac cgggcgatgt cgccatcgtc gaggcgctcg
     2821 tgcgagccat tcgctcgccg gaggggttcg agcggctcat gtcgctggaa acgttcccgc
     2881 aaacggtcgc tgccggggcc gcgcgcggga cgggctccgg ccaggcggat cgggacgccc
     2941 ccggcggtgg cgcgacaggc agcgcggcgg ctcgtcgtgg cgcggcgggg ggcgctgcca
     3001 tggacgcgcg ggccacgccc gtcgcgcccc atcgcctcgc cgacgatcac cgcgcatccg
     3061 atttcttcgt cttcgcagac gaccattgcc tcgaccggct caccgccgcg tcgaggtttt
     3121 tttgcgcccc cgggggcggc ttccttgcca cgatcccgga ttgggcgtga cgtctccgga
     3181 aagatctcgt cgcgcacgcg cgcggcccag cctcaagatg caggcgatcg cgctcctgtc
     3241 gcggcgcgag cacagtcgcc aggaactgcg cgcgaagctg ctggccgcgt tgcgccggcg
     3301 cgcgcgcgaa gacgccgccg aggctgccgc ctcgcaggcg catgccgacc cggtcgagcg
     3361 ggcgctcgcg cagacgccgt cgaacgggat cgacgacgcc ttcttgccgc gaccgcgagc
     3421 acggctgccg gtgccgccgc cccggaactc agtcgtcgtc gacagcgatg gctttctccg
     3481 gccggcgcag gagccgcaag cgccgtctca gcacgagcgg gagcaggacg ccgacccgga
     3541 agccgtcgtc gacgcgctcc tcgactggct cgccgcgcgc ggctatctga gcgacgagcg
     3601 cttcgtcgag acccgggtgc atgcgcgcgc cgcgaagcag ggcgccgcgc tgatccgcca
     3661 ggagctggcg cggcacgggc tcgcgctgca gccggaacag gcgaccgcgc tgcgggaaag
     3721 cgagttcgcg cgggcgcagg cgttgtggca gcgcaagttc ggcgaagtcg cggacgaacc
     3781 tcgcgagcgc gcccgccagg cgcgcttcct ggcctcgcgc ggcttcgcgg cggacgtcgt
     3841 ccgccgcatc gtcggcggcc aggacgagtt ctgacgcccc ggcgtggtgc gcgaagggcg
     3901 cgtgccgcac cgcgaagcct gcgcgaccgg cgggcatgtc cgggtgcctc acgtggtgca
     3961 gtgcaccatg ttaaagtcac cggttacatc gggtttccga gcattcgcgc ttgcgcgggc
     4021 ttctcccggt gcctggaatc catcgcaaaa ttccgtcttt gcgctccctc ccgcccgctg
     4081 tcgcccgtcg gccggccgct ctcgtcgtag atgcacgccg ccgcgcagcc gcctgggcac
     4141 tccctttggc cgaccacctc gagaagacgc ttccatgaag atccacgaat accaaggcaa
     4201 ggaactcctg cgccagttcg gcgtgcccgt gccgcgcggc taccccgcgt tcacggtgca
     4261 ggaggcggtg gaagccgcgc agaagctcgg cggcagcgtc tgggtggtca aggcccagat
     4321 ccacgccggt ggtcgtggca agggcggcgg cgtcaagctc gcgcggtcgc tggacgacgt
     4381 caaggcgctg gccggcgaga tcctgggcat gcagctcaag acgcaccaga ccggccccga
     4441 gggccagaag gtccgccgcc tgtacattga ggaaggcgcg gacatcaaga aggaatacta
     4501 cgtctcgctg gtgaccgacc gcgcgacgca gaaggtcgcg ctgatcgcct cgagcgaagg
     4561 cggcatggac atcgaggaag tcgcgcactc ctcgcccgag aagatcatca ccgaggtcat
     4621 cgatccgctg gccggcctga ccgccgagca ggcgaagaag gtcgcggccg ccgtcggcct
     4681 gcccgagggc tcgcaggcgc aggccgtcga cctgttccag aagctctacc agtgctacat
     4741 ggacaccgac gcctcgctgg tcgagatcaa cccgctcaac tgcgacagca agggtgcgct
     4801 gatcgccctg gacgcgaagt tcaacttcga cgccaacgcg ctgttccgcc atcccgagat
     4861 cgtcgcctat cgtgacctcg acgaagagga tccggccgag atcgaggcct cgaagttcga
     4921 cctcgcctac atctcgctgg acggcaacat cggctgcctc gtcaacggcg ccggcctcgc
     4981 gatggccacg atggacacga tcaagctgtt cggcggcgag ccggcgaact tcctggacgt
     5041 gggtggcggc gccaccgccg agaaggtcac cgaagccttc aagatcatgc tgaagaaccc
     5101 gcacgtcaag ggcatcctgg tcaacatctt cggcggcatc atgaagtgcg acaccatcgc
     5161 cgaaggcgtc atccaggcct gcaaggcggt gaacctgagc gtgcccctcg tcgtgcgcat
     5221 gaagggcacc aacgaggaac tgggcaagaa gatgctggcc gagtcgggcc tgcccatcat
     5281 ctccgccgac acgatggccg aagccgcgac caagatcgtc gccgcggtca agtaatcgag
     5341 ccccagccca gaccaaggaa tcaagcgaaa tgtcgatcta catcaacaag gacacgaagg
     5401 tcatcaccca gggcatcacg ggcaagaccg gccagttcca caccctcggc tgccaggcct
     5461 acgccaacgg caagaacgcc ttcgtcgccg gcgtgaaccc gaagaaggcg ggcgagaagt
     5521 tctcggacat ccccatctac gcgaccgtct cggaagcccg gcagcagacc ggcgcgaacg
     5581 tctccgtgat ctacgtgccg cccgcgggcg ccgccgccgc gatctgggaa gccgtcgagg
     5641 ccgacctcga cctcgtggtc tgcatcaccg agggcatccc cgtgcgcgac atgctggtcg
     5701 tgcgcaacaa gatgaaggcc aaggaggccg ccggcggcaa gaagacgctg ctgctgggcc
     5761 ccaactgccc gggcctcatc acgcccgacg agatcaagat cggcatcatg ccgggccaca
     5821 tccaccgcaa gggccgcatc ggcgtcgtct cgcgctcggg cacgctgacc tacgaggcgg
     5881 tcgcgcagct gaccgagatc ggcctgggcc agtcgtcggc ggtgggcatc ggcggcgacc
     5941 cgatcaacgg cctcaagcac atcgacgtga tgaaggcctt caacgacgac ccggagaccg
     6001 acgccgtcat catgatcggc gagatcggcg gcccggacga ggcggaagcc gcgcgctggt
     6061 gcaaggccaa catgaagaag ccggtcgtcg gattcatcgc cggcgtcacc gcgccggccg
     6121 gcaagcgcat gggccacgcc ggggcgctga tctcgggcgg cgccgacacg gccgacgcca
     6181 agctggcgat catggaggag tgcggcttca cggtcacgcg caacccgtcg gagatgggca
     6241 agctgctcaa gggcctgctc tgagctgaac gcaagcttgc gggggcggtt agactgggcc
     6301 ctcgcggcga gacgaggagg agcccgcggg ctcctttttc tttggcctgc cgcttgcctc
     6361 gcccgctctc caaccggatc gcagacgcga cccccgtcga ccgtggacct gacctccccc
     6421 gccttctgga ccgccctcgg ctccatcatc ctcgccaaca tcctgctgtc gggcgacaac
     6481 gcggtcgtga tcgcgatggc cgcgcgcgcg ctgccggcgc accagcagaa gcgggcgatc
     6541 gtgttcggca gcgtggccgc gatcgtgatg cgcgtcgtgc tcacgctgat cgcggtgaag
     6601 atgctgacgc tgccctacct gaagctggtc ggcggggcgc tgctgctgtg gatcggcgtc
     6661 aacctgctca acgaggaaga cgacggccac gcgcacggca cgcaggcgcc gggcgtggcg
     6721 gcggcgatcc gcaccatcct cgtggccgac ctcgtgatga gcctggacaa cgtcctcgcc
     6781 gttgccgccg ccgcacaggg caacacgccc ctgctggtga tcggcctcgc ggtgagcgtg
     6841 cccttgatcg tcttcggcag cacgctgctg ctgaaggtga tggagcgctt ccccttgatc
     6901 atcacggccg gcgccgcgct gctgggctgg ctggcgggcg agatgatgct gggcgacccg
     6961 ggcatcgtgc gccgcttcgg cgagatcccc gaggcctggg tgaccggcgc agggatcgcc
     7021 ggcgccgtgc tggtggtcgc ggtcggcctg gccctgaagg cgcggcggaa gcggttccag
     7081 caggcaccgg gctcctgagc ggggcgcgcc ggcgcgggcg gcggtccctg cctgccactg
     7141 tcgcgtgacg gatgccgcac agcggcgctg ggacaaaccc ggctcggatt gacgtgcgcc
     7201 atgccagccc gcacgcttcg gcccatgccg cgcatcgcgc tttccccgcc gacgcccgcg
     7261 ccgaccgccc cccgcgcggc cgcgcgtgcc tggttccgct tgagtcgcct gttcggcc
//
LOCUS       DASVLN010000170         3095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_170515, whole genome shotgun sequence.
ACCESSION   DASVLN010000170 DASVLN010000000
VERSION     DASVLN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170515"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VF453_03905"
     CDS             <1..1005
                     /locus_tag="VF453_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7686824.1"
                     /translation="LVTLALILAVNASLNPGLWHLQWRDGHLYGSLVDIVNRAAPLAI
                     VALGMTLVIATRGIDISVGAVVAISGAVAVLLLGDKLDIVGGHTVPVARVPMSLAVLA
                     ALAVALLCGAWNGLLVARVGMQPIIATLILMVAGRGAAQLLSGGQILTTYYAPYFVIG
                     NGFLLGVPVAALIALAVWGVTSLVLERSALGLFIQAIGINPRAARVAGVRSRVITVCV
                     YAFCGLCAGVAGLIVTSNIKSADGNNAGLLMELDAILAVTLGGTLLTGGRYTLAGTMI
                     GALIIQTLTSTIYAIGVPPEINLVVKAVVVFIVMLLQSADFRRQVRALVVRPPVGGAR
                     "
     gene            1126..2109
                     /gene="yjfF"
                     /locus_tag="VF453_03910"
     CDS             1126..2109
                     /gene="yjfF"
                     /locus_tag="VF453_03910"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HEX7686825.1"
                     /translation="MTAAKRSRLKVPPIVASVALLLAMVVFGGLMYTGFFSAQVFLNL
                     LIDNAFLLVVAVGMTFVILTGGIDLSVGSVVALTTMVTAALVEHAHWPPLVVIPLTLA
                     GGAVFGALQGWLIHAFRLQPFIVTLAGMFLARGLCYVISIDSISITDPTFTAIAQARI
                     ALLPGAEISVGALIALALFAVALYVAHGTAFGRNVYAIGGSEHSAVLMGLPVRATVVG
                     VYALSGFCAAMGGMLFTFYMLSGYGLHATGMELDAIASVVIGGTLLAGGVGLVAGTLC
                     GVLMLGLIQTLITFDGTLSAWWTKIVIGALLFAFCLLQRLTQAADPAAEPA"
     gene            complement(2203..2796)
                     /locus_tag="VF453_03915"
     CDS             complement(2203..2796)
                     /locus_tag="VF453_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4114 domain-containing protein"
                     /protein_id="HEX7686826.1"
                     /translation="MFLKTRLAVAALALAAAGAANAGAIPYPNPGTQNPDTYTFTASS
                     DGDLVAYFLGTGASYNEDLGVLVNGVDTGLYGLQNHSTAVDTSFNFGSVHAGDVLTFF
                     IRVQTTGDTFYSNPSLNSDGVNHVYSTSFAGDGTAPAGTYVAFEDLNGGGDLNYHDET
                     FSFVNVSTRPAVPEPANVALLLAGLGLIGTMARRRQR"
     gene            complement(2988..>3095)
                     /locus_tag="VF453_03920"
     CDS             complement(2988..>3095)
                     /locus_tag="VF453_03920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7686827.1"
                     /translation="AQASAVGFYRRAGFVAHGAPFVEAGIAHQEMRRRP"
BASE COUNT          403 a         1063 c         1114 g          515 t
ORIGIN      
        1 ctcgtcacgc tggcgctgat cctcgccgtc aacgcgagcc tgaaccccgg cctgtggcac
       61 ctgcagtggc gcgacggcca cctctacggc agcctggtcg acatcgtcaa ccgcgccgcg
      121 ccgctcgcga tcgtcgcgct cggcatgacg ctcgtgatcg ccacgcgcgg catcgacatc
      181 tcggtcggcg ccgtggtggc gatctcgggc gcggtcgcgg tgctgctgct cggcgacaag
      241 ctcgacatcg tcggcggcca caccgtgccc gtcgcccggg tgccgatgtc gctggccgtg
      301 ctcgcggcgc tggcggtggc gctgctgtgc ggcgcgtgga acgggctgct ggtcgcgcgc
      361 gtcggcatgc agcccatcat cgcgacgctc atcctgatgg tcgccggccg cggcgcggcg
      421 cagctgctgt cgggcgggca gatcctgacc acctactacg cgccctactt cgtgatcggc
      481 aacggcttcc tgctcggcgt gccggtggca gcgctgatcg cgctcgcggt ctggggcgtg
      541 acgagcctgg tgctcgagcg cagcgcgctg ggcctgttca tccaggcgat cggcatcaat
      601 ccgcgcgccg cccgcgtggc cggggtgcgc tcgcgcgtca tcacggtctg cgtctacgcg
      661 ttctgcggcc tgtgcgcggg cgtggccggg ctgatcgtga cctccaacat caagagcgcc
      721 gacggcaaca acgcgggcct gctgatggag ctggacgcca tcctcgcggt cactctgggg
      781 ggcacgctgc tgaccggcgg acgctacacc ctcgccggca ccatgatcgg cgcgctcatc
      841 atccagacgc tgacctcgac gatctacgcg atcggcgtcc cgcccgagat caacctcgtc
      901 gtgaaggccg tcgtcgtgtt catcgtgatg ctgctgcagt ccgccgactt ccggcgccag
      961 gtgcgcgcgc tggtcgtgcg gccgccggtg ggaggggcgc gatgatcgcc gcccggccgc
     1021 ccgaaggcga tcgcaccgca gtgcggagca cggaggtttc caaatgaccg ccgcccggcc
     1081 gcccgaaggc gatcgcacgg cagtgcgaag cacggaggtt tccgaatgac cgccgccaag
     1141 cgctcccggc tcaaggtgcc gcccatcgtc gcgagcgtcg cgctgctgct cgcgatggtg
     1201 gtgttcggcg gcctcatgta cacgggcttc ttcagcgccc aggtgttcct caacctgctg
     1261 atcgacaacg ccttcctgct cgtcgtcgcg gtgggcatga ccttcgtgat cctgaccggc
     1321 gggatcgacc tgtcggtggg ttcggtggtc gcgctcacga cgatggtgac ggccgcgctg
     1381 gtcgagcacg cgcactggcc gccgctggtc gtgatcccgc tgacgctcgc cggcggcgcg
     1441 gtcttcggcg cgttgcaggg ctggctgatc cacgcgttcc ggctgcagcc cttcatcgtc
     1501 acgctggccg gcatgttcct cgcacgcggg ctgtgctacg tgatcagcat cgactcgatc
     1561 agcatcaccg acccgacctt caccgcgatc gcccaggccc gcatcgcgct gctgccgggc
     1621 gcggagatct cggtgggggc gctgatcgcg ctcgcgctgt tcgccgtggc gctctacgtc
     1681 gcccacggca cggccttcgg gcgcaacgtc tacgcgatcg gcggcagcga gcactccgcc
     1741 gtcctgatgg gcctgccggt gcgcgcgacc gtggtgggcg tctatgcgct cagcggcttc
     1801 tgcgccgcga tgggcggcat gctgttcacg ttctacatgc tctcgggcta tgggctgcac
     1861 gcgacgggca tggagctcga cgccatcgcc tcggtggtga tcggcgggac gctgctggcg
     1921 ggcggggtcg gcctcgtcgc gggcacgctg tgcggcgtgc tgatgctggg gctgatccag
     1981 acgctgatca ccttcgacgg cacgctcagc gcctggtgga ccaagatcgt gatcggcgcc
     2041 ctgctgttcg ccttctgcct gctgcagcgg ctcacgcagg cggccgatcc tgccgccgag
     2101 ccggcctgac ggcgggcgcc cccatttcct gaagcgacga aggccggacg agccggcctt
     2161 cgaatgcgat gcggcgacgg gcacgcggcc cgccgcggcg catcagcgct ggcgacgacg
     2221 cgccatcgtg ccgatcaggc ccaggccggc gagcagcagc gccacgttgg ccggctccgg
     2281 cacggccgga cgggtggaga cgttgacgaa cgagaaggtc tcgtcgtggt agttcaggtc
     2341 gccgccgccg ttcaggtcct cgaaggcgac gtaggtgccg gccggcgcgg tgccgtcgcc
     2401 cgcgaagctc gtcgagtaga cgtggttcac gccgtcgctg ttcagggacg ggttcgagta
     2461 gaaggtgtcg ccggtcgtct ggacgcggat gaagaacgtc agcacgtcgc ccgcgtggac
     2521 gctgccgaag ttgaacgagg tgtcgacggc cgtcgaatgg ttctgcaggc cgtacaggcc
     2581 cgtgtccacg ccgttgacca ggacgccgag gtcctcgttg taggaggcgc cggtgcccag
     2641 gaagtaggcc accaggtcgc cgtcggacga ggcggtgaag gtgtacgtgt ccgggttctg
     2701 cgtgccgggg ttcggatacg ggatcgcgcc ggcgttggcg gcgccggccg cggcgagggc
     2761 cagcgcggcg acggcgaggc gggtcttgag gaacatgcga ggactccaac ggttgcttgt
     2821 ggaacttccg cccggcgcaa gcgggtaagc ccgcagagaa ttcgccttcg gcgtgaagga
     2881 ttgggcgact ttctcaacca cggttcagtt gtacgatacg ggttttcccc catgcctcac
     2941 cagatccggt gaaaaggccc cacgcggcgc gggccgtcac gaccgcgtca cgggcggcgc
     3001 cgcatctcct ggtgcgcgat gccggcctcg acgaacggcg cgccgtgcgc gacgaagccg
     3061 gcgcggcgat agaagcccac cgcgctggcc tgcgc
//
LOCUS       DASVLN010000171         6883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_170577, whole genome shotgun sequence.
ACCESSION   DASVLN010000171 DASVLN010000000
VERSION     DASVLN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170577"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VF453_03925"
     CDS             complement(<1..963)
                     /locus_tag="VF453_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX7686828.1"
                     /translation="MERNLGLDVLRLALALMVIGLHAELLAGVSPMWNHLLVDGLFRA
                     AVPVFLVINGYYFFDTVQRGRVRAWLTGTAGLYAFWTIAYLPLHAPGSAADRHAWARF
                     AVDLVLGFWHLWYVAALLAAGAVLALLRRWSSRTLLALAAALYLAGCALQLETLYGSH
                     AFSNRIYRSFLLDALPFLVVGYVIRREDFRPSLGACLAAACAGAAGVMAESLASFVWS
                     ARSGNVDLFFSLALLGPALFLAARHVEVAAPTRGLAKVASSVYFVHVAILVLAGRWLH
                     LEQGPALFGLVALASLGLSPLVVALARRFPVVLGGAGRAVQAPAS"
     gene            complement(1091..3892)
                     /gene="metH"
                     /locus_tag="VF453_03930"
     CDS             complement(1091..3892)
                     /gene="metH"
                     /locus_tag="VF453_03930"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX7686829.1"
                     /translation="MSSPVSPVPAPAAIPVPPPMRLSGLEPVSLEPGHLFVNIGERTN
                     VTGSKAFARMILAGEFEKALAVARQQVENGAQVIDVNMDEAMLDSQAAMVRFLNLVAS
                     EPEIARVPVMVDSSKWSVIEAGLKCLQGKGIVNSISMKEGVDEFKRQARLIRRYGAAT
                     VVMAFDEQGQADTFARKTEICERAYRILVDEVGFPPEDIIFDPNIFAIATGIEEHDNY
                     AVDFIEATRWIKRNLPGAKVSGGVSNVSFSFRGNEPVREAIHTVFLYHAIKAGMDMGI
                     VNAGMVGVYDNLEPRLRELVEDVVLNRQPAYKDGEPRLTPTERLLEIAEEVKGAVRDD
                     SARLAWRAGTVAQRLSHALVQGITDFIVEDTEEAYREIVAKGGRPLHVIEGPLMDGMN
                     VVGDLFGAGKMFLPQVVKSARVMKSAVAHLVPYIEEEKKRLEAAGADVRSKGKIVIAT
                     VKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVSCQDILARAKAERADIIGLSGLITPSL
                     EEMQYVASELQRDPDLRAMKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVG
                     VCSDLLSQERSDKFLEELEADYARVREQHANRKQVPLVTLEAARANRTPLDWDAYTPP
                     APKFIGRRLFKAYDLAEIAACIDWGPFFQTWDLAGPYPKILDDEIVGEAARKVFADGQ
                     AMLRRIVEGRWLTANGVFGFWPANTVGDDTIEIYTDESRSEVLLRWSPLRMQTERPVV
                     PGPDGRDMRRPNRSLADFIAPKGTKPDWIGLFAVTAGLGVDKREAEFQAAHDDYSSIM
                     LKALADRLAEAFAERLHQRVRTELWGYAPDERLSNEALIAEQYRGIRPAPGYPACPEH
                     GIKRALFRALQADEAGMALTESLAMTPAASVSGFYLAHPQSTYFNVGRIGEDQLLDWA
                     AREDVPEAEARRRLSSALG"
     gene            3982..5199
                     /locus_tag="VF453_03935"
     CDS             3982..5199
                     /locus_tag="VF453_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686830.1"
                     /translation="MKVVPLNSEGLRVGRPLPFSVRDGSGAVLLARGASIQTDKQLQL
                     LRARPLFIDYAESEAVRRAVNGELDRMFLQDAALGRIASAQPNYDVVAEKLAATARAA
                     AQAEAQLDWPNLQMRLRLLLVDPRGADWIARLRALRDDVLQLLERNPDRALVRLTYDA
                     GSDFQDYSASHSLYVCVLVALSCVVVPGWDPAWRDPLTLAALTMNLTFTTTQDELARQ
                     AGALNDNQKAMLRDHPEQAARLLAEIGVDDPLWLHAVRHHHSAPPGPVVGRAPGAVLA
                     RLVRRADRYAARLSPRKSRPASSATAAAQVALLDEGGQTDEAGQALIRTLGLYPPGAW
                     VKLQCGEIALVLRRTAIAKAPVVASFVNRSGMPLAVPALRNTRLPDYAVTGAVPPAQV
                     KVRPNFEMLAKMA"
     gene            complement(5245..6300)
                     /locus_tag="VF453_03940"
     CDS             complement(5245..6300)
                     /locus_tag="VF453_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX7686831.1"
                     /translation="MTARPLPFTRAARLPELLKQRIVVIDGAMGTMIQRYKLHEADFR
                     GTLLPDHPKDVKGNNELLQLVRPDVLREIHSQYLAAGADIIETNTFGATSVAQEDYGL
                     AHLAREMNLAAARLARECADRYETPDKPRFVAGALGPTPRTASISPDVNDPGARNIHF
                     DELRAAYREQAEGLVEGGADLFLVETIFDTLNAKAAIFALDELMEETGVRLPVIISGT
                     VTDASGRILSGQTVTAFWHSVRHARPVAVGLNCALGAALMRPYIEELAKVAGDTAISC
                     YPNAGLPNPMSETGFDETPEVTGALMEEFAQAGFLNIVGGCCGTTPEHIAAIARRVSA
                     YRPRGVHDPLFSQLAAA"
     gene            complement(6348..6809)
                     /locus_tag="VF453_03945"
     CDS             complement(6348..6809)
                     /locus_tag="VF453_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686832.1"
                     /translation="MRIWKRRAALALLAAAGLASLAGCGGDVYWDDGGWADFDVIAAV
                     DGHRVHGFDAFPGDEQVVHAVVGDAVELDSSGWVDWTIAAGDGPDTSTHAGDTFQVDE
                     VTVSETVVNSGQIALSLSATAPLAAPVTVTIYAISRDDPDQVARIDIVVSD"
BASE COUNT          984 a         2464 c         2462 g          973 t
ORIGIN      
        1 gctggccggc gcctgaaccg cgcgccccgc gccgcccagc acgaccggga agcggcgcgc
       61 cagcgcgacc accagcggcg acaggcccag gctggcgagc gcgaccaggc cgaacagcgc
      121 cggtccctgc tccaggtgca gccaacggcc ggcgagcacg aggatcgcca cgtgcacgaa
      181 gtacaccgag gacgcgacct tggcgagccc ccgcgtcggc gccgcgacct ccacgtgtcg
      241 cgccgccagg aacagcgcgg gcccgagcaa ggccagcgag aagaagaggt cgacgttgcc
      301 gctgcgcgcc gaccagacga agctcgcgag actctcggcc atcacgcccg cggcgccggc
      361 gcacgccgcc gccaggcagg cgcccagcga aggccggaag tcctcgcggc ggatgacgta
      421 accgacgacg aggaagggca gggcgtcgag gaggaaactg cggtaaatcc ggttggagaa
      481 cgcgtggctg ccgtagagcg tctcgagctg gagcgcgcac ccggcgaggt agagcgccgc
      541 cgcgagcgcc agcagcgtcc ggctcgacca ccggcgcagc agggcgagca ccgccccggc
      601 ggcgagcagc gccgcgacgt accagagatg ccagaagccg agcacgagat cgaccgcgaa
      661 gcgggcccac gcgtggcggt cggcggccga cccgggcgcg tgcaacggca ggtaggcgat
      721 ggtccagaac gcgtacagcc cggcggtgcc cgtgagccat gcgcgcacgc ggccgcgctg
      781 gacggtgtcg aagaagtagt agccgttgat gacgaggaac accggcacgg cggcgcggaa
      841 caacccgtcg accagcaggt ggttccacat cgggctcacg ccggcgagca gctccgcgtg
      901 caggccgatg accatcagcg cgagcgcgag ccgcagcacg tccagaccga ggtttctttc
      961 catcgccgag cccgccaggt tgcggcggcg cgggtcgatg acaagcctgc gcgcgccgag
     1021 ttgaacggcg cacggtagca atccggatgc ccggatgggt atccctaggg acgcggctcg
     1081 cgcgcgccgc tcacccgagc gccgagctca accggcggcg ggcctccgcc tcgggcacgt
     1141 cctcgcgcgc ggcccagtcg agcagctggt cctcgccgat gcgcccgaca ttgaagtagg
     1201 tcgactgcgg atgcgcgagg tagaagcccg agacgctcgc cgccggggtc atcgccaggc
     1261 tctcggtgag cgccatgccc gcctcgtcgg cctgcagggc ccggaacagc gcgcgcttga
     1321 tgccgtgctc gggacaggcc gggtagccgg gcgccgggcg gatgccccgg tactgctcgg
     1381 cgatcagcgc ctcgttggac agtcgctcgt cgggtgcgta gccccacagc tcggtgcgca
     1441 cgcgctggtg caggcgctcg gcgaaggcct cggccaggcg gtcggcgagc gccttgagca
     1501 tgatcgacga atagtcgtcg tgcgctgcct ggaactcggc ctcgcgcttg tcgacgccca
     1561 ggccggccgt gaccgcgaac aggccgatcc agtccggctt ggtgcccttg ggggcgatga
     1621 agtcggcgag cgagcggttc gggcgccgca tgtcccgccc gtccggaccg ggcacgaccg
     1681 ggcgctcggt ctgcatgcgc agcggcgacc agcgcagcag cacctcggag cgcgactcgt
     1741 cggtgtagat ctcgatcgtg tcgtcgccga ccgtgttcgc cggccagaag ccgaacacgc
     1801 cgttggcggt gagccagcgg ccctcgacga tgcgccgcag catcgcctgg ccgtccgcga
     1861 acaccttgcg cgcggcctcg ccgacgatct cgtcgtcgag gatcttcggg tacggcccgg
     1921 ccaggtccca ggtctggaag aacgggcccc agtcgatgca ggccgcgatc tcggcgaggt
     1981 cgtaggcctt gaacaggcgc cggccgatga acttcggtgc cggcggcgtg tacgcgtccc
     2041 agtccagcgg cgtcctgttg gcgcgcgccg cctccagcgt caccagcggc acctgcttgc
     2101 ggttggcgtg ctgctcgcgc acgcgcgcgt agtcggcctc gagctcctcg aggaacttgt
     2161 cggagcgctc ctgcgagagc aggtcggagc acacgcccac cgagcgcgac gcgtccggga
     2221 cgtagacgac cggtccctcg tagtgcggcg cgatcttgac ggcggtgtgg actcggctgg
     2281 tcgtcgcgcc gccgatcagc agcgggatct tcatcgcgcg caggtcgggg tcgcgctgca
     2341 gctcggaggc gacgtactgc atctcctcca ggctcggggt gatcaggccc gacaggccga
     2401 tgatgtcggc gcgctcggcc ttcgcgcggg cgaggatgtc ctggcaggac accatcacgc
     2461 ccatgttgac gacctcgaag ttgttgcact ggaggacgac ggtgacgatg ttcttgccga
     2521 tgtcgtggac gtcgcccttg accgtcgcga tcacgatctt gcccttggag cgcacgtccg
     2581 cgccggcggc ctcgaggcgc ttcttctcct cctcgatgta gggcacgagg tgggccaccg
     2641 ccgacttcat cacccgcgcc gacttcacca cctggggcag gaacatcttg ccggcgccga
     2701 acaggtcgcc gacgacgttc atgccgtcca tcagcggccc ctcgatcacg tgcagcggcc
     2761 gcccgccctt cgcgacgatc tcccggtagg cctcctcggt gtcctcgacg atgaagtcgg
     2821 tgatgccctg gacgagcgcg tgcgacaggc gttgcgccac cgttcccgcg cgccaggcga
     2881 gccgtgccga gtcgtcgcgc acggcgccct tgacctcctc ggcgatctcc agcaggcgct
     2941 ccgtgggcgt caggcgcggc tcgccgtcct tgtaggccgg ctggcggttc agcaccacgt
     3001 cctcgaccag ctcgcgcagc cgcggctcga ggttgtcgta gacgcccacc atgccggcgt
     3061 tgacgatgcc catgtccatg cccgccttga tcgcgtggta caggaacacg gtgtggatcg
     3121 cctcgcgcac gggctcgttg ccgcggaacg agaagctcac gttggagacg ccgcccgaga
     3181 ccttcgcgcc gggcaggttg cgcttgatcc agcgcgtcgc ctcgatgaag tcgacggcgt
     3241 agttgtcgtg ctcctcgatg cccgtcgcga tcgcgaagat gttcgggtcg aagatgatgt
     3301 cctcgggcgg aaagccgacc tcgtcgacca ggatgcggta tgcgcgctcg cagatctcgg
     3361 tcttgcgcgc gaaggtgtcg gcctggccct gctcgtcgaa ggccatgacc accgtcgcgg
     3421 cgccgtagcg gcggatcagc cgcgcctggc gcttgaactc gtcgacccct tccttcatcg
     3481 agatcgagtt gacgatgccc ttgccctgca ggcacttcag gccggcctcg atgacgctcc
     3541 acttcgacga gtcgaccatc accggcacgc gcgcgatctc gggctcgctg gccacgaggt
     3601 tgaggaagcg caccatggcg gcctggctgt ccagcatcgc ctcgtccatg ttgacgtcga
     3661 tgacctgggc gccgttctcc acctgctgac gcgcgacggc cagcgccttc tcgaactcgc
     3721 cggccaggat catgcgtgcg aaggccttgg aacccgtgac gttcgtgcgc tcgccgatgt
     3781 tgacgaacag gtgccccggc tccagcgaga ccggctcgag gccggacagc cgcatcggcg
     3841 gcgggacggg aatggcggcg ggcgccggca cgggcgaaac gggcgaggac atgtgccaaa
     3901 ttgtaggcgc cgcccctcaa gcgctcggcc gggcagccga tattccggat ccgtacaccc
     3961 cctttcgggc attctcgatc catgaaggtc gtcccgctca attccgaagg cctgcgcgtc
     4021 ggccgcccgc tgccgttctc ggtgcgcgac ggctcgggcg ccgtgctgct cgcgcgcggc
     4081 gcctcgatac agaccgacaa gcagctgcag ctcctgcgcg cgcggccgct gttcatcgac
     4141 tacgccgagt ccgaggccgt gcggcgggcc gtcaacggcg aactggaccg gatgttcctg
     4201 caggacgcgg cgctcggccg catcgccagc gcgcagccca actacgacgt cgtcgccgag
     4261 aagctcgccg cgaccgcccg cgcggccgcg caggccgagg cccagctgga ctggcccaac
     4321 ctgcagatgc ggctgcggct gctgctggtc gacccgcgcg gcgccgactg gatcgcccgc
     4381 ctgcgcgcgc tgcgcgacga cgtcctgcag ctgctggaac gcaaccccga ccgcgcgctg
     4441 gtgcgcctga cctacgacgc gggcagcgac ttccaggact acagcgcgag ccacagcctg
     4501 tacgtgtgcg tgctggtcgc gctcagctgc gtcgtcgtgc cgggctggga tcccgcgtgg
     4561 cgcgacccgc tgacgctcgc cgccctgacg atgaacctca cgttcacgac gacccaggac
     4621 gagctcgccc gccaggccgg cgcgctcaac gacaaccaga aggcgatgct gcgcgaccat
     4681 ccggagcagg ccgcgcggct gctggccgag atcggcgtcg acgacccgtt gtggctgcac
     4741 gcggtgcggc accatcacag cgcgcccccg gggccggtgg tcggtcgcgc gccgggcgcg
     4801 gtgctcgcac gcctggtgcg ccgcgccgac cgctacgcgg cccgcctgag cccgcgcaag
     4861 agccggccgg cgtcttccgc gacggcggcg gcgcaggtcg cgctgctcga cgagggcggg
     4921 cagaccgacg aggccggcca ggcgctgatc cgcacgctgg gcctctatcc gcccggcgcg
     4981 tgggtgaagc tgcagtgcgg cgagatcgcg ctcgtcctgc gccgcaccgc aatcgccaag
     5041 gcgccggtcg tcgcgagctt cgtcaaccgc tccggcatgc ccctggccgt acccgcgctg
     5101 cgcaacaccc ggctgccgga ctacgcggtc accggcgcgg tgccgccggc gcaggtcaag
     5161 gtgcgtccca acttcgagat gctcgcgaag atggcctgac ggccggccgg ccgcttcccg
     5221 cgcatcgggc gggccgtcgg cgcctcaggc cgccgcgagc tgcgagaaca gcggatcgtg
     5281 caccccgcgc gggcggtagg cgctgacgcg gcgcgcgatc gcggcgatgt gctcgggcgt
     5341 cgtcccgcag cagccgccga cgatgttgag gaagcccgcc tgcgcgaact cctccatcag
     5401 cgcgccggtg acctcgggcg tctcgtcgaa gccggtctcg ctcatcgggt tgggcaggcc
     5461 ggcgttcggg tagcagctga tcgccgtgtc gccggcgacc ttggcgagct cctcgatgta
     5521 ggggcgcatc agcgccgccc ccagcgcgca gttcaggccg accgccaccg gccgcgcgtg
     5581 gcgcaccgag tgccagaacg cggtcaccgt ctggcccgag aggatgcggc ccgacgcgtc
     5641 cgtcaccgtg cccgagatga tcaccggcag gcggacgccg gtctcctcca tcagctcgtc
     5701 gagcgcgaag atcgcggcct tggcgttgag cgtgtcgaag atggtctcga cgaggaacag
     5761 gtcggcgccg ccctcgacga gcccttcggc ctgctcgcgg taggcggcgc gcagctcgtc
     5821 gaagtggatg ttgcgcgcgc ccgggtcgtt gacgtcgggg ctgatgctgg ccgtgcgcgg
     5881 cgtcgggccg agcgcgcccg cgacgaagcg cggcttgtcg ggtgtctcgt agcggtcggc
     5941 gcactcgcgc gcgaggcgcg cggcggcgag gttcatctcg cgcgccaggt gcgcgaggcc
     6001 gtagtcctcc tgcgccaccg aggtcgcgcc gaaggtgttg gtctcgatga tgtccgcgcc
     6061 cgccgcaagg tactgcgaat ggatctcgcg caggacgtcg ggccgcacca gctgcagcag
     6121 ctcgttgttg cccttcacgt ccttcgggtg gtccggcagc agcgtgccgc ggaagtcggc
     6181 ctcgtgcagc ttgtagcgct ggatcatcgt gcccatggcg ccgtcgatga cgacgatgcg
     6241 ctgcttcagc agctcgggca ggcgggcggc gcgcgtgaag ggcagggggc gggcggtcat
     6301 ggctggcatt ctaaggagtc gccctgcccg cggcggccgg cgggggatca gtccgacacg
     6361 acgatgtcga tgcgcgcgac ctggtccggg tcgtcgcgcg agatcgcgta gatcgtcacc
     6421 gtcaccggcg ccgcaagcgg ggcggtcgcc gacagcgaca gcgcgatctg ccccgagttg
     6481 acgaccgtct cgctgacggt cacctcgtcg acctggaagg tgtcgccggc gtgggtggag
     6541 gtatcggggc cgtcgccggc cgcgatcgtc cagtcgaccc atcccgacga atccagctcg
     6601 accgcgtcgc cgacgacggc atgcacgacc tgctcgtcgc ccgggaaggc gtcgaagccg
     6661 tgcacgcggt ggccgtcgac ggcggcgatg acgtcgaaat cggcccagcc gccatcgtcc
     6721 cagtaaacgt ccccgccgca gccggccagg cttgccagcc ccgccgccgc gagcagcgcc
     6781 agcgcggcgc gtcgcttcca gatccgcatc gctcgtctcc ttcgactgtc gccgggcgcg
     6841 cgtcgacgac cgccggcgcc cgtggggcgg acccggcaat cgg
//
LOCUS       DASVLN010000172         2139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_170615, whole genome shotgun sequence.
ACCESSION   DASVLN010000172 DASVLN010000000
VERSION     DASVLN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170615"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF453_03950"
     CDS             <1..327
                     /locus_tag="VF453_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686833.1"
                     /translation="FVTRERVAAGPGEEGDTAWRIALIQKDPRRGARGRVSLAGLPGA
                     ALAKVASARLETWRCADVFDSSDERALWQHADEALAPASTFDLRLPPASLALLTINLK
                     AFPHAG"
     gene            324..1511
                     /locus_tag="VF453_03955"
     CDS             324..1511
                     /locus_tag="VF453_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7686834.1"
                     /translation="MTTSISSLAGQARRPRIGVDFHTFDGLFQGSRSHLLGIYREAIR
                     RAPEYDFVFFCADPEALRAADPAFAMPNAYIVAMPHKGGLARLAWQLAWRQWRSRLDL
                     LHVQYRMPLVPLGACAVTIHDALYETHPQFFDKRFVRMARLTGRLAARRAKVLLTVSE
                     FSRAQIASLYRVDPLQVTITGNGVNLARFHPAEEGGSEEWNGAEIVRALGVEPGEYLL
                     TVGRLEPRKNHLGLVRAYAQLSEPRPPLLIVGQRDFGCDEVFAEVAALGLQDGVKLLE
                     NVADAELPALLRHARVFVYPSFAEGFGMPVLEAMASGVAVVTSDTTALAEVAAGAALT
                     ADPGDPKAIAGRIEQLLFDGKLRRGLAARGLEVAARHRWDTAAKALLGAFRRYLASVP
                     GRG"
     gene            1587..2045
                     /locus_tag="VF453_03960"
     CDS             1587..2045
                     /locus_tag="VF453_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7686835.1"
                     /translation="MTPSSTDIVRKQVSLKASPARVWQALADAREFGAWFRVNVEGPF
                     VVGQPAEGFITHPGYEHLRWRIVVEAMEPESRFAFRWHPYAVDPAIDYASEAPTHVEF
                     RLETEGSGTLLTVTESGFDRLPAHRRELAYRMNDGGWAAQMDNLRAHVDA"
BASE COUNT          276 a          801 c          757 g          305 t
ORIGIN      
        1 ttcgtcacgc gcgagcgcgt cgccgccggc cccggggagg agggtgatac ggcgtggcgt
       61 atcgcgctga tccagaagga tccgcgccgc ggcgcgcgcg ggcgcgtctc gctcgcgggc
      121 ctgccgggcg cggcgcttgc caaggtcgcg tcggctcgcc tggaaacctg gcgctgcgcc
      181 gacgtgttcg actcgagcga cgagcgcgcg ctctggcaac atgcggacga ggcgctcgcg
      241 cccgcgtcca cgttcgacct gcggctgccg ccggcgagcc tggctctgct cacgatcaac
      301 ctcaaggcct tcccccacgc cggatgacga cctccatctc ctcgcttgcc ggccaggcgc
      361 gccgcccccg catcggcgtc gacttccaca cgttcgacgg cctgttccag ggcagccgca
      421 gccacctgct cgggatctat cgcgaggcga tccggcgcgc gcccgagtac gacttcgtgt
      481 tcttctgcgc cgaccccgag gcgctgcgcg cggccgatcc cgcgttcgcg atgccgaacg
      541 cgtacatcgt cgcgatgccg cacaagggcg gcctcgcgcg cctggcctgg cagctcgcct
      601 ggcggcagtg gcgcagccgg ctcgacctgc tgcacgtgca gtaccgcatg ccgctcgtgc
      661 cgctgggcgc ctgcgcggtg accatccacg acgcgctcta cgagacccat ccgcagttct
      721 tcgacaagcg cttcgtgcgc atggcgcgcc tgacgggccg gctcgcggcg cggcgcgcga
      781 aggtgctgct gacggtcagc gagttctcgc gcgcccagat cgcgagcctc tatcgcgtcg
      841 atcccctgca ggtgacgatc accggcaacg gcgtcaacct cgcgcgcttc catccggccg
      901 aggagggcgg cagcgaagaa tggaacggcg ccgagatcgt gcgcgcgctg ggcgtcgagc
      961 ccggcgaata cctcctcacc gtcggccgcc tggagccgcg caagaaccac ctgggcctcg
     1021 tgcgggcgta cgcgcagctg tccgagccgc ggccgccgct gctgatcgtc ggccagcgcg
     1081 acttcggctg cgacgaggtc ttcgccgagg tcgccgccct gggcctgcag gacggcgtga
     1141 agttgctcga gaacgtcgcc gacgccgagc tgccggcgct gctgcgccac gcgcgcgtgt
     1201 tcgtgtaccc gagcttcgcg gagggcttcg gcatgcccgt gctggaggcg atggccagcg
     1261 gcgtggcggt cgtgacgtcg gatacgacgg cgctcgccga ggtcgcggcc ggcgccgcgc
     1321 tcacggccga tccgggcgat ccgaaggcga tcgccggccg catcgagcag ctgctgttcg
     1381 acggcaagct gcgccgcggc ctggccgcgc gcgggctcga ggtcgcggcg cggcatcgct
     1441 gggacaccgc ggccaaggcg ctgctcggcg cgttccggcg ctacctcgcg tcggtgccgg
     1501 gccgcggttg agcgccgtct cgatacgcaa ccaaatggtt gcatatcgac ggcgatcggc
     1561 gtacaaaggc gtccaaggag acgcccatga ccccttcctc gaccgacatc gtccgcaagc
     1621 aggtctcgct gaaggcgtcg cccgcgcgcg tctggcaggc gcttgccgac gcgcgcgagt
     1681 tcggcgcctg gttccgcgtg aacgtcgaag gcccgttcgt cgtcggccag ccggccgagg
     1741 gcttcatcac gcatccgggc tacgagcacc tgcgctggcg catcgtcgtg gaggcgatgg
     1801 agcccgagtc gcgcttcgcc ttccgctggc atccgtacgc ggtcgacccg gcgatcgact
     1861 acgcgtcgga agcgcccacc cacgtcgagt tccggctcga gacggaaggc agcggcacgc
     1921 tgctcacggt caccgagtcc ggcttcgacc gcctgcccgc gcaccggcgc gagctcgcgt
     1981 accgcatgaa cgacggcggc tgggcggcgc agatggacaa cctgcgcgcc cacgtcgacg
     2041 catgacgccg cggcacgccc gcaccctccc gcccgcgccg gcggacgccg ccgcgctgtt
     2101 cgcggcgctc ggcgaccaga cgcggctcgc gctgctggc
//
LOCUS       DASVLN010000173        11293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_223201, whole genome shotgun sequence.
ACCESSION   DASVLN010000173 DASVLN010000000
VERSION     DASVLN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223201"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..3033)
                     /locus_tag="VF453_03965"
     CDS             complement(142..3033)
                     /locus_tag="VF453_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lanthipeptide synthetase LanM family
                     protein"
                     /protein_id="HEX7686836.1"
                     /translation="MTAPVMGAGDFEASLGCLFASDLDELAAQLFSIPGLDDTERTTI
                     AQETRANLVATLHGKLSRMLLLELNAARLRGQLAGETPEQRWAEFLALSSRPAFWDDI
                     ACEYPEMRGRVARIVAHRCANSLRFARRFAADRRALDDFAGAPLGALESIGFGAGDTH
                     QQGQTVAVVRCTGGRVVYKPRSLAVDAALGAFIDELFADDASPHRIRVPRVLERDGYG
                     WTAFVEHRHAADDDELRSFYLGIGHWLALMRLVGGSDLHSENLIAHGGTPVVVDCETL
                     FTPRLPQIPAGLGNAVDRAMEVTNSTVLASGLLPGRSGALGWRGVDSSALGGLPGQQP
                     RVRKIEIVGAGTDEARIGTDEREIEQSQNLPGTSPALATFWPDVVRGFDELTARLQAL
                     DAEGALAPRMARFKSCRIRVVPRATEVYAEIGRMLWHPVSLHDQAAAVARGVDLLRRM
                     AARVRIAPHIHEIVEAEVADLLDGDIPFYTTIAGHGVLDGPRGTTWLKPSDLVEEALD
                     AWRVADFALERRVIRAALVSAYINEGWIPPEGQMKPARVDTADLDARRRRQAAAIMRE
                     LVASRIDGDDGTVSWIAPVLNPDGWSVQPLEQDLYNGASGLLLLVAGYEREVAAGRAD
                     PVDGVAALLPRLQKTLRCAELQREKQTREVKHPRPGSPGAYIGLGSQIWALLTLRRLG
                     LDPGECLERACGLASFLPASVAADEVHELLVGKAGAIVPLLMLAAATGQRAYVDMAVD
                     IGDRLVGAARVRETDGAMCWPQQKWPEGVGGFAHGATGISWALAKLARASGEPRFADA
                     SSAAARFEDALWNEAEGNWTDLRFVDGVGTAAAWCHGSVGIGLAQLDLDPALAAPGAR
                     ERVRRAADATWRMGMGWNHSLCHGDLGAAELLVQAVDRGLAPAGMTRDSLLAWILGSM
                     ERHGAVCGIVRDTFSPGLMPGLGGVAYQLLRMHPGCDLPSVLVLGGGPS"
     gene            complement(3041..3235)
                     /locus_tag="VF453_03970"
     CDS             complement(3041..3235)
                     /locus_tag="VF453_03970"
                     /inference="COORDINATES: protein motif:HMM:NF040361.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6229 family protein"
                     /protein_id="HEX7686837.1"
                     /translation="MNTTQRTVDADALIAQWRAGSEESPAGPLFSGGEYAEADIVGDI
                     VNFTAQCGTGCSASRKQYCC"
     gene            4025..4654
                     /locus_tag="VF453_03975"
     CDS             4025..4654
                     /locus_tag="VF453_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEX7686838.1"
                     /translation="MNILQVNSSARAWNNGQGSQSTRLASELVAALRAADPASSVVVR
                     DLARTPHPALDEATLGALFTPTEHRTPEQQARVALDDALIAELRAADVVVLGVPMINF
                     GVTSQLKNWFDAVAKARVTFAYTDKGPVGLLTGKKVYAVLTRGGVHRDTPNDSQVPYL
                     RTMLAFLGMTDVEFVYAEGLSMGPEAEARALASARDEIARIAGGAPVAA"
     gene            4716..5624
                     /locus_tag="VF453_03980"
     CDS             4716..5624
                     /locus_tag="VF453_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7686839.1"
                     /translation="MTTILDTPSAAVPVRRPRTVERLVAGQSTRDGDGVKLTRVLTQP
                     LQRRLDPFLMLDAFGSDDAADYIGGFPDHPHRGFETVTYMIAGRMRHRDSFGGEGLLE
                     NGGVQWMTAGRGLIHSELPEQDEGRMEGFQLWLNLPAADKMGEPGYRDIASGEIPGFT
                     RAGAHVRVIAGESHGVAGAVRRAGTEPLYLDLHLEPGARFEQALPAGHNAFVYVYRGA
                     LDVEGADASTAVPVQRMAVLANGADRDGVRLRAGEAGARALLIAGRPLGEPIAQYGPF
                     VMNTQAEIFQAVEDFRAGRFGAPPGR"
     gene            complement(5711..7072)
                     /locus_tag="VF453_03985"
     CDS             complement(5711..7072)
                     /locus_tag="VF453_03985"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX7686840.1"
                     /translation="MKKREFLAVGGAMPLMLAGCGGSGSGSAPVRLVNASVGYPSLGF
                     MVETTQATSSDVAYGSASPFETVQAGSVTMTLTVGGSSVVSTNRNLGKDTRYTLVAYG
                     FKDELKSLLVTESTVAPDSGRANINVLNTSVDIGSVDVYMQTTNTTDLSLATQIASSV
                     TPQTQSPFYSALPGTYYITVVGAGSLANGKTDVRFRSTGTITIADQDIVTVILTPGAS
                     GTLANAIVLFQQNGPDGSKGATSFLNTQARLRVVTAVPNGTVVAVGPLPNSTTNQYLL
                     PASNKSQVSGYADVATGTPPPITYNGSALTVTGAITQLAAGGDYTLAIYPNGGTPTAT
                     LFLDDNTAPLGSAGVKYRLMNLAYDNPTSALRMTVNSKAVANNVVFPSASTYTEVATP
                     QTIATVATVSDGAQAIYTIPQQVLVSGNVFTEVIVSYTVAGDGTPVVSQFTLSADETL
                     TIV"
     gene            7287..9395
                     /locus_tag="VF453_03990"
     CDS             7287..9395
                     /locus_tag="VF453_03990"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEX7686841.1"
                     /translation="MPAPTPEPDASATPYPKLLSPLDLGFTTLRNRTVMGSMHTGLED
                     AARDFPRMAAFYAERARGGAGLIVTGGFAPNFEGWVKPFAGTLWTHGGARRHRQVTGA
                     VHAEGGKIALQILHAGRYGYSPLCVAPSRIQSPISPFTPRALGARGVERQIRAFVRCA
                     VLAREAGYDGVEIMGSEGYLINQFLVTHTNRREDAWGGSYENRMRLPVEIVRRVREAV
                     GPDFIVIYRLSMIDLIPDGSSWDEAVQLAKAVAAAGATILNTGIGWHEARVPTIVTSV
                     PRAAFGWVTGKMRAELRAAGLGIPLVTSNRINTPEVAEGLLAPAPDQFGQADLVSLAR
                     PFLADPAFVAKAAAGRADEINTCIACNQACLDHTFRNRQASCLVNPRAGYETELVLRP
                     TASPRRFAVVGAGPAGLAAATSLAERGHRVVLFDAADRIGGQFNMARRIPGKEEFHET
                     LRYFRRRIEALPTLELRLGTRVDAAALAAGGFEAVVLATGVVPRDPRIPGQDHPKVLS
                     YIDVLRHGKPVGDRVAIVGAGGIGFDVAEFLATGADRGLPSPSLHLEDWKREWGVADP
                     AEHRGGVVPPQPDAPVRRITLLQRKAGKHGAGLGKTSGWVHRATLKMKGVEMLGGVNY
                     EAVTDEGLAVSFGPERKDGTVLAVDTIVLCAGQEPLRELAAPLRAAGVAVHLIGGADE
                     AAELDAKRAIDQGTRLAATL"
     gene            9556..10251
                     /locus_tag="VF453_03995"
     CDS             9556..10251
                     /locus_tag="VF453_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEX7686842.1"
                     /translation="MANPSAFWFAITRLGESEILLPAFLAGALWMAFARPAGALGRIA
                     GGVAHAHDHPARLAAWRWIAGLFTTTWLTLATKVAFLGFGIGSAALDFTGFSGHSMYS
                     TAILPVLGAVVAGRRGAVAGGLLALVVTVSRVQVGAHSWSEAVTGLALGGAVAWWTLD
                     DYLARPGAVRAPWWLPVLLAAWLTVLPWRGPPSQTHDVVVAIALKVSGHDRPYTRFEL
                     LAGLIRPMPVRSS"
     gene            10323..10814
                     /locus_tag="VF453_04000"
     CDS             10323..10814
                     /locus_tag="VF453_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7686843.1"
                     /translation="MAHEPRPVPSFAARSYCWEDFAPGRVFELGSTRVEREAVLAFAR
                     DFDPQPFHLDDAAAEASLFGRLAASGWHTCAMVMRLMCDGYLNDSASLGSPGIDELRW
                     LKPVYPGDTLHVRMEVLESRPMKSKPHVGLVLSRWRVTNQDGVDVMTMQGWGMFGRRT
                     PAG"
     gene            11024..>11293
                     /locus_tag="VF453_04005"
     CDS             11024..>11293
                     /locus_tag="VF453_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7686844.1"
                     /translation="MSHTCYHPAFEQADIRYAIGVDGGGTGTRARLWTSGGALIGHGE
                     SGPSALAQGIEQAWKHIGQAVERAFASAGLAAPVPRHCVVGLGLAG"
BASE COUNT         1577 a         4050 c         4041 g         1625 t
ORIGIN      
        1 ccgcctcgcg caggatctcc acgatcggcc gcaaggccat gcgcaggcgc gaccaggcca
       61 accgcgcgcc cgcgggcgcg tcggccggcg cctcgatgcg ccagtacggc gtctcgaggt
      121 cgggtgcggc ggcctcggag ctcaggacgg gcctccgccg agcaccagca ccgagggcag
      181 gtcgcagccg ggatgcatgc gcagcagctg gtaggcgact ccgcccagcc ccggcatgag
      241 gccgggcgag aaggtgtcgc gcacgatgcc gcagacggcg ccatggcgct ccatgctgcc
      301 caggatccag gcgagcagcg agtcgcgcgt catgcctgca ggcgcgaggc ccctgtcgac
      361 ggcctgcacg agcagctcgg cggcgccgag gtcgccgtgg cacaggctgt ggttccagcc
      421 catgcccatg cgccaggtgg cgtccgcggc gcggcgcacg cgctcgcgcg cgcccggggc
      481 ggccagagcg ggatcgagat ccagctgcgc gaggccgatg cccaccgagc cgtggcacca
      541 cgccgccgcg gtgccgacgc cgtcgacgaa gcgcaggtcc gtccagttgc cttcggcctc
      601 gttccacagc gcgtcctcga agcgcgcggc ggcggacgag gcgtcggcga agcgcggctc
      661 gccgctcgcc cgcgccagct tggccagcgc ccacgagatg cccgtcgcac catgtgcgaa
      721 cccgcccacg ccttcgggcc acttctgctg cggccagcac atcgcgccgt cggtctcgcg
      781 gacgcgggcc gccccgacca ggcggtcgcc gatgtcgacg gccatgtcga cgtaggcgcg
      841 ctggccggtc gcggccgcga gcatcagcag cggcacgatc gcgccggcct tgccgaccag
      901 cagctcgtgc acctcgtcgg cggcgaccga cgccggcagg aagctcgcga ggccgcacgc
      961 ccgctccagg cactcgcccg gatcgagccc gaggcggcgc agcgtgagca gcgcccagat
     1021 ctgcgagccg aggccgatgt aggcgcccgg cgagccgggt cgcggatgct tcacctcgcg
     1081 cgtctgcttc tcgcgctgga gctcggcgca gcgcagcgtc ttctgcagcc gcggcagcag
     1141 cgccgcgacg ccgtcgaccg gatccgcgcg ccccgccgcg acctcgcgtt catagcccgc
     1201 gaccagcagc agcagccccg acgcgccgtt gtacaggtcc tgctccagcg gctgcaccga
     1261 ccagccgtcc gggttgagca ccggcgcgat ccacgagacc gtgccgtcgt cgccgtcgat
     1321 gcggctcgcg accagctcgc gcatgatcgc cgcggcctgc cggcggcggc gcgcgtcgag
     1381 gtcggcggtg tccacccgcg caggcttcat ctgtccttcg ggcgggatcc agccctcgtt
     1441 gatgtacgcg ctgacgagcg cggcgcggat cacgcgtcgc tcgagcgcga agtccgcgac
     1501 gcgccaggcg tccagcgctt cctcgaccag gtccgacggc ttcagccacg tcgtgccgcg
     1561 cgggccgtcg aggacgccgt ggcccgcgat cgtcgtgtag aacgggatgt cgccgtccag
     1621 caggtcggcc acctccgcct cgacgatctc gtggatgtgc ggcgcgatgc gcacgcgggc
     1681 ggccatccgc cgcagcaggt cgacgccgcg cgccacggcg gccgcctggt cgtgcagcga
     1741 gacgggatgc cacagcatgc ggccgatctc ggcatacacc tcggtcgcgc gcggcaccac
     1801 gcggatgcgg caggacttga agcgcgccat ccgcggtgcg agcgcgcctt cggcatcgag
     1861 cgcctgcagg cgcgcggtga gctcgtcgaa gccccgcacg acgtccggcc agaacgtggc
     1921 gagcgccggg ctcgtgccgg gcaggttctg cgactgctcg atctcccgct cgtcggtgcc
     1981 gatgcgcgcc tcgtcggtgc ccgcgccgac gatctcgatc ttgcgcacgc gcggctgctg
     2041 gccgggcagc ccgccgagcg cggagctgtc gacgccgcgc cagcccagcg cgccgctgcg
     2101 cccgggcagc aggccgctcg cgagcaccgt gctgttggtg acctccatcg cgcggtcgac
     2161 cgcgttgccc aggccggccg ggatctgggg cagccgcggc gtgaacagcg tctcgcagtc
     2221 gaccaccacc ggcgtgccgc cgtgcgcgat caggttctcg gagtgcaggt cgctgccgcc
     2281 gacgagccgc atcagggcga gccagtggcc gatgccgagg tagaagctgc gcagctcgtc
     2341 gtcgtccgcg gcgtggcggt gctcgacgaa cgcggtccag ccgtagccgt cgcgctccag
     2401 gacgcgcggc acgcggatgc ggtggggcga ggcgtcgtcg gcgaacagct cgtcgatgaa
     2461 ggcgcccagc gcggcgtcga cggccagcga acgcggcttg tagaccacgc ggccgccggt
     2521 gcagcgcacc accgcgacgg tctggccttg ctggtgggtg tcgccggcgc cgaagccgat
     2581 cgactcgagc gcgccgagcg gcgcgccggc gaagtcgtcg agcgcccggc gatcggcggc
     2641 gaagcgtcgc gcgaagcgca ggctgttggc gcagcggtgc gccacgatgc gcgccacgcg
     2701 gccgcgcatc tcgggatatt cgcaagcgat gtcgtcccag aacgcgggac gtgaagaaag
     2761 cgccaggaac tcggcccagc gttgctcggg cgtctcgccg gccagttgcc cacgaaggcg
     2821 cgcggcgttg agttccagca gcagcatgcg cgagagcttg ccgtgcagcg tcgccacgag
     2881 gttcgcgcgc gtctcctgcg cgatcgtcgt gcgttccgta tcgtccaggc cggggatcga
     2941 gaaaagctgg gctgccagct cgtccaggtc ggacgcgaac aggcagccca gcgatgcttc
     3001 gaagtcaccg gcacccatga caggcgcggt catgacttct tcagcagcag tattgcttgc
     3061 gcgaggcgct gcagccggtg ccgcactggg cggtgaagtt gacgatgtcg ccgacgatgt
     3121 cggcttccgc gtactcgccg ccggagaaca gcgggccggc cggggactct tcggaaccgg
     3181 cacgccattg ggcgatcagg gcatcggcat cgacggtgcg ttgcgtggtg ttcatgcgga
     3241 actccttgat ttagggatgt gatggaacgt gccgaggcga cgccctcccc ttcggccgac
     3301 gtggatgccg accattcgcg gaacgctcct cccctgcacg gcgcgcaggt tgcaacagcg
     3361 gtcgtgtcgt cacaagcgct ggaaaacccg ctccggaatt gcacgtccct tgcaaacacg
     3421 gattgacgtc gaaccgcgga atttccatga accggctaat ttcgcctaaa aggtaaccaa
     3481 ataattcagg cgccgcttgg gggtggtcga ccagaggtac tgatacgcgt acgggtacag
     3541 gtgtcatgaa ctgattcgac gcaaaacgat gactgctctg ttgcacatgc ttacgtcaat
     3601 agattggcca atctgctcgc tcacgacttt ccgtataagt ctcggcaaga catgaattac
     3661 ggaaggcttg gccatcgata attccatggc cgcgacattc ggccgaacaa gtcacgcagc
     3721 attcgatcgc cgcacgccgc gattgcgcgt ctgcgctgtc cgatttcaat gtcacgagcg
     3781 cgctgaaaac ggcgccgggc gcgtatccct cctttggggg gagcgtgtcg cacgcgatgc
     3841 ggggcgcccg tgagttcgag cttcaacgcg ccggcgccgc cgagccagcg acagctcgga
     3901 tcgtcccgcc agtggaacaa tcaaacccaa atcaacgact tccgtcgcag gatcgtcgtc
     3961 aatacagtgg cttccatcga ccggcacccc gccggccgcg tcaaacgacc cggaaggaca
     4021 cgccatgaac atcctgcaag tcaactccag cgcccgcgca tggaacaacg gccagggttc
     4081 gcaatcgacc cgtctcgcga gcgaactcgt cgccgcgctg cgggctgccg atcccgcgag
     4141 ctcggtcgtg gtccgcgacc tcgcccgcac gccgcatccg gcgctcgacg aggcgacgct
     4201 gggcgcgctg ttcacgccca ccgagcaccg cacgccggag cagcaggcgc gggtggcgtt
     4261 ggacgacgcg ctgatcgccg agctgcgcgc cgccgacgtg gtcgtgctgg gcgtgcccat
     4321 gatcaacttc ggcgtgacca gccagctcaa gaactggttc gacgccgtcg cgaaggcgcg
     4381 cgtgaccttc gcctacaccg acaagggccc ggtcggcctg ctcaccggca agaaggtcta
     4441 cgccgtgctg acccggggtg gcgtgcaccg cgacacgccc aacgacagcc aggtccccta
     4501 cctgcgcacg atgctcgcgt tcctgggcat gacggacgtc gaattcgtct acgccgaggg
     4561 cctgagcatg ggcccggagg ccgaggcgcg ggcgctggcg tcggcgcgcg acgagatcgc
     4621 ccgcatcgcc ggcggggcgc cggtcgcggc ttgaggcgca tcgcatccac cgggcgcgtg
     4681 cctcgcggcg cgcgtccttt cacaggagat cccgcatgac cacgatcctc gacaccccgt
     4741 ccgccgccgt cccggtgcgc cgtccgcgca cggtggagcg cctcgtcgcg ggccagtcca
     4801 cccgcgacgg cgatggcgtg aagctcacgc gcgtgctgac gcagccgctg cagcggcgcc
     4861 tcgacccgtt cctgatgctg gacgcgttcg gctcggacga cgcggccgac tacatcggcg
     4921 gcttccccga ccacccgcat cgcggcttcg agacggtcac ctacatgatc gcgggccgca
     4981 tgcgacaccg cgactcgttc ggcggcgaag gcctgctcga gaatggcggc gtgcagtgga
     5041 tgacggccgg ccgcggcctg atccacagcg agctgcccga gcaggacgag ggccgcatgg
     5101 aaggcttcca gctgtggttg aacctgcccg cggccgacaa gatgggtgag ccgggctacc
     5161 gcgacatcgc gagcggcgag atccccgggt tcacgcgcgc cggcgcccac gtgcgggtga
     5221 tcgcgggcga gagccacggc gtggccggcg cggtgcgcag ggccggcacc gagccgctgt
     5281 acctcgacct gcacctggag ccgggcgcgc gcttcgagca ggcgctgccc gcgggccaca
     5341 acgcgttcgt ctacgtgtac cgcggcgcgt tggacgtcga aggcgccgac gcgtcgaccg
     5401 ccgtgcccgt ccagcgcatg gcggtgctgg ccaacggcgc cgaccgcgac ggcgtgcgcc
     5461 tgcgtgccgg cgaggcaggg gcgcgcgcgc tgctgatcgc ggggcgtccg ctgggcgagc
     5521 cgatcgccca gtacggcccg ttcgtgatga acacgcaggc cgagatcttc caggcggtcg
     5581 aggacttccg cgccggccgc ttcggcgcgc caccgggccg gtgaccgtcg acgcgcctgc
     5641 ggcgcttcca tgaaaaatgg cgcctcgagg gcgccattcg tcttcgcggg cccgcgggcc
     5701 gcgcgcgagg tcagacgatc gtgagcgtct cgtcggccga cagcgtgaac tgcgacacca
     5761 ccggcgtgcc gtccccggcc acggtgtagg agacgatcac ttcggtgaac acgttgccgc
     5821 tgaccagcac ctgctgcggg atcgtgtaga tcgcttgcgc gccatcggac accgtcgcca
     5881 cggtcgcgat cgtctgcggc gtggcgactt ccgtgtaggt ggacgcgctc gggaacacga
     5941 cgttgttcgc gacggccttc gagttgaccg tcatccgcag cgcggacgtc gggttgtcgt
     6001 aggccaggtt catcagccgg tacttgacgc ccgccgaacc caggggtgcc gtgttgtcgt
     6061 cgaggaacag cgtcgccgtg ggcgtgccgc cgttcgggta gatggccagc gtgtagtcgc
     6121 cgccggcggc cagctgggtg atggcgcccg tgacggtgag ggccgagccg ttgtaggtga
     6181 tcggcggcgg cgtgcccgtg gccacatccg cgtatcccga gacctgcgac ttgttggagg
     6241 ccggcagcag gtactggttg gtcgtcgagt tcggaagcgg gccgacagcc accaccgtgc
     6301 cattgggcac cgcggtcacc acgcgcaggc gcgcctgcgt gttcaggaag ctcgtcgcgc
     6361 ccttgctgcc gtccgggccg ttctgctgga acagcacgat cgcgttggcg agcgtgccgc
     6421 tggcgcccgg ggtcaggatg acggtgacga tgtcctggtc ggcgatcgtg atcgtgccgg
     6481 tcgagcggaa gcggacgtcg gtcttgccgt tggccaggct gccggcgccc acgacggtga
     6541 tgtagtaggt gcccggcagc gccgagtaga acgggctctg cgtctgcggc gtcacgctgg
     6601 aggcgatctg cgtggcgagc gacaggtcgg tggtgttggt cgtctgcatg tagacgtcca
     6661 ccgagccgat gtccaccgag gtgttcagca cgttgatgtt ggcgcggccc gagtcggggg
     6721 cgacggtgct ctccgtgacc agcagcgact tcagctcgtc cttgaagccg taggcgacca
     6781 gcgtgtagcg ggtatccttg cccaggttgc ggttcgtcga gacgacgctg gagccgccga
     6841 ccgtcagcgt catcgtgacg ctgccggcct ggacggtctc gaacgggctc gcgctgccgt
     6901 aggcgacgtc ggaggacgtg gcctgagtcg tttcgaccat gaaccccagc gacggatagc
     6961 caacgctcgc gttgaccagt cggacgggtg cgctgccgga gccactgccg ccgcaaccgg
     7021 cgagcatcag gggcatcgcg ccgcccacgg ccaggaactc acgttttttc atcgaaacac
     7081 tccacttttc caggcgagcg cctgtcatct cgaatccgcc attatgcggc agaccatgtt
     7141 aaccgcccgt tgccgcgttg ccggcgcact gcgcggagag cctgcatcgc gcaacttcgc
     7201 acgggtggaa acccggcgaa ggctctagac ggacaccccg atttccggcc gtccgcaggc
     7261 gccgcgacgc gtctacgatc gcggccatgc ccgccccgac gcccgagccc gacgcctccg
     7321 ccacgcccta tccgaagctg ctgtcgccgc tggatctcgg cttcacgacg ctgcgcaacc
     7381 gcaccgtcat gggcagcatg cataccggcc tggaggacgc cgcgcgcgac ttcccgcgca
     7441 tggccgcctt ctacgccgag cgggcgcgcg gcggcgcggg gctgatcgtc accgggggct
     7501 tcgcgcccaa tttcgagggc tgggtcaagc ccttcgcggg cacgctgtgg acgcacgggg
     7561 gcgcgcgccg ccatcgccag gtcaccggcg ccgtgcacgc cgagggcggc aagatcgcgc
     7621 tgcagatcct gcatgccggg cgctacggct actcgccgct gtgcgtggcg ccgagccgca
     7681 tccagagccc catctcgccg ttcacgccgc gcgcgctggg cgcccgcggc gtcgagcgcc
     7741 agatccgcgc cttcgtgcgc tgcgcggtgc tcgcgcgcga ggcgggctac gacggcgtcg
     7801 agatcatggg cagcgagggc tacctgatca accagttcct cgtgacgcac accaaccggc
     7861 gcgaggacgc ctggggcggc tcctacgaga accggatgcg gctgccggtg gagatcgtgc
     7921 gccgcgtgcg cgaggcggtc ggcccggact tcatcgtcat ctaccggctg tcgatgatcg
     7981 acctcatccc cgacggcagc agctgggacg aggcggtgca gctcgccaag gcggtggccg
     8041 ccgcgggcgc gacgatcctg aacaccggca tcggctggca cgaggcgcgg gtgccgacca
     8101 tcgtgaccag cgtgccgcgc gcggccttcg gctgggtcac cggcaagatg cgcgcggagc
     8161 tgcgcgccgc cgggctcggg atcccgctgg tcacgagcaa ccggatcaac acgcccgagg
     8221 tcgccgaggg gctgctcgcg ccggcgccgg accagttcgg ccaggccgat ctcgtctcgc
     8281 tcgcgcggcc cttcctcgcg gatccggcgt tcgtcgccaa ggccgcggcc ggccgggccg
     8341 acgagatcaa cacctgcatc gcctgcaacc aggcctgcct cgatcacacc ttccgcaacc
     8401 ggcaggcgag ctgcctcgtc aacccgcgcg ccggctacga gacggagctc gtgcttcgcc
     8461 cgaccgcgag ccccaggcgc ttcgccgtcg tcggtgccgg gccggcaggg ctcgccgccg
     8521 ccacctcgct ggcggagcgc ggccaccggg tcgtgctgtt cgacgccgcc gaccgcatcg
     8581 gcggccagtt caacatggcc aggcgcatcc ccggcaagga ggagttccac gagacgctgc
     8641 gctacttccg ccgccgcatc gaggcgctgc cgacgctgga gctgcgcctg ggcacgcggg
     8701 tggacgccgc ggccctggcc gcgggcggct tcgaggcggt ggtgctcgcc accggcgtgg
     8761 tgccgcgcga tccccgcatc ccgggccagg accatcccaa ggtgctcagc tacatcgacg
     8821 tgctgcggca cggcaagccg gtgggcgatc gcgtggcgat cgtcggcgcg ggcggcatcg
     8881 gcttcgacgt cgccgagttc ctcgccacgg gcgccgaccg cgggctgccg tcgcccagcc
     8941 tgcacctgga ggactggaag cgcgaatggg gcgtcgcgga tccggcggag caccgcggcg
     9001 gcgtcgtccc gccgcagccc gacgccccgg tgcgccggat caccctgctg cagcgcaagg
     9061 ccggcaagca cggcgccggg ctgggcaaga cctccggctg ggtgcaccgc gcgacgctga
     9121 agatgaaggg cgtcgagatg ctcggcggcg tgaactacga ggccgtcacg gacgagggcc
     9181 tggccgtgag cttcggcccc gagcgcaagg acggcacggt gctggcggtc gacacgatcg
     9241 tgctgtgcgc cggccaggag ccgctgcgcg agctggccgc cccgctgcgg gcggccggcg
     9301 tcgcggtgca cctgatcggc ggcgccgacg aggccgccga actcgacgcc aagcgggcga
     9361 tcgaccaggg cacgcggctc gcggcgacgc tgtgagcgcg aacgggcgct aaaccagcgc
     9421 gcgcgccggc cgatgatcgg ggagcgccgc gcgggcgtgg cgccgcctcg tccgcttcac
     9481 attcgcctca cgcgcattcg ctagcatgcc gccgatgcga cgcttcatcc ctccgaccgt
     9541 gccccgatga ttcccgtggc caatccgtcc gcgttctggt tcgctatcac gcggctgggc
     9601 gaatccgaga tcctgctgcc cgccttcctc gccggcgcgc tgtggatggc cttcgcgcgc
     9661 ccggccggcg cgctcggtcg catcgccggc ggcgtcgcgc acgcccacga ccaccccgcc
     9721 cgcctggcgg cctggcgctg gatcgccggc ctgttcacga ccacctggct gacgctcgcc
     9781 accaaggtcg cgttcctggg cttcggcatc ggctccgcgg cgctggactt caccggcttc
     9841 tccgggcact cgatgtactc gacggcgatc ctgccggtgc tcggcgcggt ggttgccggt
     9901 cgccgcggcg cggtggccgg cgggctgctc gccctcgtcg tcacggtgtc gcgggtgcag
     9961 gtgggcgcgc attcgtggag cgaggccgtc accgggctcg cgctgggcgg cgccgtcgcc
    10021 tggtggacgc tggacgacta cctggcccgt cccggcgccg tgcgcgcgcc ctggtggctg
    10081 ccggtgctgc tcgccgcgtg gctgaccgtg ctgccctggc gcggcccgcc gtcgcagacg
    10141 cacgacgtgg tggtggcgat cgcgctgaag gtctccggcc acgaccggcc gtacacgcgc
    10201 ttcgagctgc tcgccggcct gatccggccg atgccggtgc gctcgagctg acgcggcccc
    10261 gccgcccgcg cgggcggccg cgaggcgcta tgctggccgc tcgaccgcca cagtccgagc
    10321 ccatggccca cgaaccgcgc cccgtcccat ccttcgccgc ccggagctat tgctgggagg
    10381 acttcgcgcc cgggcgcgtc ttcgagctcg gcagcaccag ggtcgagcgc gaggccgtgc
    10441 tggcgttcgc gcgcgatttc gacccccagc ccttccacct cgacgacgcc gcggcggagg
    10501 cctcgctgtt cggccgcctg gccgcgagcg gctggcacac ctgcgcgatg gtgatgcggc
    10561 tgatgtgcga cggctacctc aacgactcgg ccagcctcgg ctcgcccggc atcgacgaac
    10621 tgcgctggct caagccggtc tacccgggcg acaccctgca cgtgcgcatg gaggtgctgg
    10681 agtcgcgccc gatgaagagc aagccgcacg tcggcctcgt gctgtcgcgc tggcgggtga
    10741 cgaaccagga cggcgtcgac gtgatgacga tgcagggctg gggcatgttc gggcggcgca
    10801 cgccggcggg ctgacgggtt gccccggcga cggcgggcag cgccggggcg gacttgattg
    10861 gtattaccac tggttcatgc gagcgtcgcg gtccatgcgg cgcgccgttt gatgcttccg
    10921 gccgggaatt ccacctcggg gaacaaattc cggggaagca tcggccgccc gcagcgtcgc
    10981 gttgacaagc ggtctatcat ggtactatag tggtatcaca cctatgagcc acacctgcta
    11041 tcaccccgcc ttcgaacagg ccgacatccg ctacgccatc ggcgtcgatg gcggcggcac
    11101 gggcacccgc gcccggctct ggacgagcgg cggcgcgctg atcggccacg gcgagtccgg
    11161 accgtcggcg ctcgcgcagg gcatcgagca ggcgtggaag cacatcggcc aggcggtgga
    11221 gcgcgcgttc gcgtcggccg gcctcgcggc gccggtgccg cggcactgcg tcgtcggcct
    11281 cggcctggcg ggc
//
LOCUS       DASVLN010000174         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_223258, whole genome shotgun sequence.
ACCESSION   DASVLN010000174 DASVLN010000000
VERSION     DASVLN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223258"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VF453_04010"
     CDS             <1..433
                     /locus_tag="VF453_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7686845.1"
                     /translation="AAAAGPAAPDFTLRAADGRNVRLDELRGQVVLVNFWATWCGPCR
                     EEMPHLNALYEKYRKSGFVLLGVNIDDNPATAIATAGKLAVGFPILLDTDKQVSKLYK
                     LTDMPSTAIIDRDGRLRYVHRGFREGVEADYDQQIRALLKE"
     gene            436..705
                     /locus_tag="VF453_04015"
     CDS             436..705
                     /locus_tag="VF453_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HEX7686846.1"
                     /translation="MPELRLAPLANLAGLARLAAACGLAAVLGACAFKPIEPWVKPYE
                     RERLADPVMKADVNELPARQRDHIYTVREGARGATGVQGGGCGCN"
     gene            692..1798
                     /locus_tag="VF453_04020"
     CDS             692..1798
                     /locus_tag="VF453_04020"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEX7686847.1"
                     /translation="MAATRLNGAAAVAGLALAAGAAQAVDLPEDRAETMYHYYDGGGT
                     KAQGPAVLIRKSMMDRVALQAGYYVDAVSNASIDVVTTASPYKETRNEFDLGLDYVVR
                     DTLIKVSGSRSKEPDYLADTFNLDLSQDVFGGMTTVAFGFSRGQDKVGQHGVSGWIDS
                     ARHWQYRVGATQILTPRWQMSANLEAIDDDGYLGSPYRVARVFGAAIPERDPRTRASH
                     ALKLSTTGDITPEGATNRSSVRVDYRYFWDTWGIRAHTLELGYNRYFGPQWLMESFLR
                     YYRQGHALFYSDDAQAETLYVTRNRQLSTMHDVSAGATITYTLKDVPGKYDVKLSGSY
                     QLIDFRYSDFTDIRTGKPYSNHANVIQTVLSATF"
BASE COUNT          323 a          739 c          628 g          257 t
ORIGIN      
        1 cgcagccgcc gccggccccg ccgcacccga cttcacgctg cgcgccgccg acggccgcaa
       61 cgtccgcctc gacgaactgc gcggccaggt cgtgctcgtg aacttctggg ccacgtggtg
      121 cggcccgtgc cgcgaggaga tgccgcacct gaacgcgctg tacgagaagt accgcaagtc
      181 cggcttcgtg ctgctgggcg tcaacatcga cgacaacccc gccaccgcga tcgcgaccgc
      241 gggcaagctg gcggtgggat tccccatcct gctggacacc gacaagcagg tcagcaagct
      301 gtacaagctc accgacatgc cctcgacggc catcatcgac cgcgacggcc gcctgcgcta
      361 cgtgcaccgc ggcttccgcg agggcgtcga ggccgactac gaccagcaga tccgcgcgct
      421 gctcaaggaa tgacgatgcc cgagctccgc ctcgcccctc tcgccaacct cgccggcctc
      481 gcgcggctcg ccgccgcctg cggcctggcc gccgtgctgg gcgcctgcgc gttcaagccg
      541 atcgagccct gggtcaagcc ctacgagcgc gagcgcctcg ccgaccccgt catgaaggcc
      601 gacgtcaacg agctgcccgc caggcagcgc gaccacatct acaccgtgcg cgagggcgcg
      661 cgcggcgcca ccggcgtgca ggggggcggc tgtggctgca actaggctca acggcgcggc
      721 ggcggtcgcg ggcctcgcgc tcgccgcggg cgcggcgcag gcggtcgatc tccccgagga
      781 tcgcgccgag acgatgtacc actactacga cggcggcggc acgaaggcgc agggcccggc
      841 ggtgctgatc cgcaagtcga tgatggaccg cgtcgcgctg caggccggct actacgtgga
      901 cgcggtcagc aacgcgtcga tcgacgtcgt cacgaccgcg agcccgtaca aggagacgcg
      961 caacgagttc gacctcggcc tcgactacgt ggtgcgcgac acgctcatca aggtgagcgg
     1021 ctcgcgcagc aaggagccgg attacctggc cgacacgttc aacctcgacc tgtcgcagga
     1081 cgtcttcggc ggcatgacca cggtcgcctt cggcttcagc cgcggccagg acaaggtcgg
     1141 acagcacggc gtgagcggct ggatcgactc ggcgcggcac tggcagtacc gcgtcggcgc
     1201 gacgcagatc ctcacgccgc gctggcagat gagcgccaac ctcgaggcga tcgacgacga
     1261 cggctacctc ggcagcccat accgcgtcgc gcgggtgttc ggcgcggcga ttcccgaacg
     1321 cgacccgcgc acgcgcgcga gccacgcgct caagctgtcg acgaccggcg acatcacgcc
     1381 cgagggcgcg accaaccgca gctcggtgcg cgtcgactac cgctacttct gggacacctg
     1441 gggcatccgc gcccacacgc tggagctcgg ctacaaccgc tatttcggcc cgcagtggct
     1501 gatggagagc ttcctgcgct actaccgcca gggccatgcc ctcttctaca gcgacgacgc
     1561 gcaggcggag acgctatatg tcacgcgcaa ccgccagctc agcaccatgc acgacgtgag
     1621 cgccggcgcg acgatcacct acacgctcaa ggacgtcccc ggcaagtacg acgtcaagct
     1681 gagcggctcg taccagctga tcgacttccg gtactcggac ttcaccgaca tccgcaccgg
     1741 caagccctat tcgaaccacg ccaacgtgat ccagaccgtg ctgtcggcca cgttctgaca
     1801 tcgtccaaga gagcccgtcc atgaagcaac cccttcgcgc ctgcctcgcg ctcgccgcgg
     1861 cgatcggttt ctgcgccgcc gcggccttcg tcgccgcgcc ggtgcacgcg gcggacgcac
     1921 cgtcgcccgc agcggccgcc gcgcccc
//
LOCUS       DASVLN010000175         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_439228, whole genome shotgun sequence.
ACCESSION   DASVLN010000175 DASVLN010000000
VERSION     DASVLN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439228"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VF453_04025"
     CDS             complement(<1..505)
                     /locus_tag="VF453_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7686848.1"
                     /translation="MLRVEGLAKRYGAGLVFSGVSLAAARGELVALLGESGVGKSTLL
                     NCIAGLDTADAGTITLAGRRVLELDEPAAARLRRETLGFVFQAFHVLPHLNVADNVGL
                     PLQLLGRPRGDRVTHILAAVGLEGLEERAPRQLSGGQLQRVAIARALVHEPALVLADE
                     PTGNLDPR"
     gene            915..1712
                     /locus_tag="VF453_04030"
     CDS             915..1712
                     /locus_tag="VF453_04030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7686849.1"
                     /translation="MTLENDSSALTGLVRVLERRSTRFWGAMAIAVAVAAVAVATGLG
                     MLAGGRAAAVALSVAVFFALVALGLAGTVIRLARTVADSRARQVLHAVDDDLTGLSNR
                     RHFLELVEREFARCRRYGDDGALLLIDADHLRRINEHLGQLCGDALLLEVTKRVGATL
                     RQPDLLARFGGAELAVFLPNTDPLGALDAAERIRQQVASSPYQWQQTHVVATVSIGVA
                     TVHQGQLSVDTMVHEAEQALEAAKEAGRNCVRTGPIKPRKSGERVAS"
     gene            1866..>2195
                     /locus_tag="VF453_04035"
     CDS             1866..>2195
                     /locus_tag="VF453_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7686850.1"
                     /translation="MRRLILKMSMSLDGFVAGPDGEIDWMFPDRDAGSTAWVLETLRA
                     AGLHAMGSRTLRDMAAWWPTARDPMAEPMNAIPKVVFTRRGLAAGAGDTTTALRDAGR
                     QRAAEGEA"
BASE COUNT          292 a          782 c          823 g          298 t
ORIGIN      
        1 tgcgcgggtc gaggttgccg gtgggctcgt cggcgagcac cagcgccggc tcgtgcacga
       61 gggcccgggc gatggccacc cgctgcagct ggccgccgga cagctgccgc ggcgcgcgct
      121 cctcgaggcc ttcgagcccg accgccgcga ggatgtgcgt gacgcggtcg ccgcgcggcc
      181 gcccgagcaa ctgcagcggc aggccgacgt tgtccgcgac gttcaggtgc ggcaggacat
      241 ggaaggcctg gaacacgaat ccgagcgtct cgcgtcgcag gcgggcggcg gccggctcgt
      301 cgagctcgag cacgcggcgg ccggccagcg tgatcgtccc cgcatcggcg gtgtccaggc
      361 cggcgatgca attgagcagc gtggacttgc ccacgccgga ttcgccgagc agcgcgacca
      421 gctcgccgcg cgccgccgcg agcgacacgc cgctgaacac gagtcccgcg ccatagcgct
      481 tggcgaggcc ctcgacccgc agcaacggtt cggaggggcc ggcggttcgg gcggaagggg
      541 cgggggcgtc catgcggcag cgaggatacg cgaagccgtg cccccggccc gcgcgggaga
      601 cgccgcgccg gcggctcgca ggccggtcga tcgcgacctg ccaagtcccg gacggtctgc
      661 aagattgcgc atttcgtccc cgggcgtggc ggcgctcggg ctgcggcagc gccgaccctg
      721 ccgcacaatc gacccggaac cggccatgcg aacgggccac cgggggcccg cccgggggcg
      781 ccggcgtggc ggggacggat agccggcgcg gtcgcgaggg gatatgctgg aatggcccgc
      841 gtcatcgggc tctcaaattg cccgcgcgtt ggtcgaatac cgttcgacgc tttgcttttt
      901 tctggggtgg atgggtgacg cttgaaaacg actcctcggc gctgaccggc ctcgtgcggg
      961 tgctcgagcg ccgcagcacg cgcttctggg gtgccatggc catcgcggtg gcggtcgcgg
     1021 ccgtggcggt ggcgacgggg ctgggcatgc ttgcgggggg ccgcgcggcg gccgtcgcgc
     1081 tgagcgtggc cgtgttcttc gcgctggtgg cgctgggcct ggcgggcacg gtgatccgcc
     1141 tcgcgcgcac ggtggccgac tcgcgcgcac gccaggtgct gcacgcggtc gacgacgacc
     1201 tgacgggcct gtcgaaccgg cgccacttcc tggagctcgt cgagcgcgag ttcgcgcgtt
     1261 gccgccgcta cggcgacgat ggcgcgctgc tgctgatcga cgccgaccac ctgcgccgga
     1321 tcaacgagca cctcggccag ctgtgcggcg acgcgctgct gctggaggtc accaagcgcg
     1381 tcggcgcgac gctgcgccag ccggacctgc tcgcccgctt cggcggggcc gagctcgcgg
     1441 ttttcctgcc caacaccgat ccgctgggcg cgctcgacgc cgccgagcgc atccgccagc
     1501 aggtggcgtc ctccccctac cagtggcagc agacccacgt ggtcgccacg gtcagcatcg
     1561 gcgtggccac cgtccaccag ggccagctga gcgtcgacac catggtgcac gaggccgagc
     1621 aggccctgga ggccgccaag gaggccggcc gcaactgcgt gcgcaccggc ccgatcaagc
     1681 cgcgcaagag cggcgagcgc gtcgccagct gacgccgccg gggcctttcc cgacatcctc
     1741 gcgacccgcc ctcgagcggg tcgcttcgtt ccagggtttc taccgagatt aaaacatacc
     1801 gagtagttgg tatgcttttg ccatcgagtc gacgtaccgg tcgaccgcca cgaaaggacg
     1861 ctgccatgag acgcctgatc ctgaagatgt cgatgtcgct ggacggcttc gtggccgggc
     1921 cggacggcga gatcgactgg atgttccccg accgcgatgc cggctcgacg gcctgggtgc
     1981 tggagaccct gcgtgccgcg ggcctgcacg cgatgggcag ccgcaccctc cgcgacatgg
     2041 cggcctggtg gccgaccgcc cgggatccga tggccgagcc gatgaacgcg attcccaagg
     2101 tcgtcttcac ccggcgcggc ctggctgccg gcgccggcga caccaccacc gcgctgcgcg
     2161 acgccgggcg gcagcgcgcg gccgagggcg aggcc
//
LOCUS       DASVLN010000176         4821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_116333, whole genome shotgun sequence.
ACCESSION   DASVLN010000176 DASVLN010000000
VERSION     DASVLN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116333"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VF453_04040"
     CDS             <1..704
                     /locus_tag="VF453_04040"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEX7686851.1"
                     /translation="DLPLPIARHLARALAHAGEPPFAVRLAHRGEFDVSPRRVAWRAF
                     TSRQWMLGSRPGFVWAARMSMLPAVAVDVLDAYVDGVAELRADLWGWLPLSKARATPA
                     IARGELMRWLAESVLVPWAWRTCPGLRWRPVDDRHADATLADGAVDVALHFAFGADGL
                     AESVHADSRPRMVGGGIVETPWNGRWSDYRTCDGVLVPTRGEVEWRIDGRRKPYLRAA
                     LARYEGVQRAGELHA"
     gene            complement(725..1150)
                     /locus_tag="VF453_04045"
     CDS             complement(725..1150)
                     /locus_tag="VF453_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7686852.1"
                     /translation="MSALTRFDPMDEFFPEFFRRLARPLSFDAGLPAEIRLDVSENEK
                     EYLVSAEVPGARKDDVRVEIDRNQVTISAEVRKDVEDKHADGGRALLRETWRGRTSRT
                     FTLAQDVDDKAASAKLEDGVLRLTLPKRAGAASKLLSIQ"
     gene            1360..2016
                     /locus_tag="VF453_04050"
     CDS             1360..2016
                     /locus_tag="VF453_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7686853.1"
                     /translation="MMKSDAQIKSDVEAELAWDPLVDEAHVGVSVRDAVVELTGTLGS
                     HAERLAAERAALRVGGVQGVTVALRVAPPAGCERADADVVKACQHALAWTADVPEGMV
                     RLRVEKGRVTLVGDVDWAYQRQAAEMAVCDLLGVVAVVNELRVRARPAETDIRAGIRA
                     ALERQADRDAERIDVHVDGSCVMLSGRARTWAEREAALAAAWSAAGVTQVVNEVVVRP
                     "
     gene            2081..4567
                     /locus_tag="VF453_04055"
     CDS             2081..4567
                     /locus_tag="VF453_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7686854.1"
                     /translation="MTASPPSELPSPWQELLAGAGLMVGVVDQAGRVLLANRAMRDAC
                     GRGDAGASGLQWPRDFVATVDQAEAAEALARWIGGDPAAGAVELSPLERGGAAVRRVR
                     WHGTSWSTAAGVALLGVDVSAERAQEAKLLRTTRFYRALSDMNAAVGRLQDASALYRA
                     VCEIAVDSGHALMAWVGLRDGEFLAPVAWGGNARDYTRGLALRLEGGPQPRGPSSDAF
                     ASGRPSICNDVDTDPRMAPWREHARAFGVRSSAAFPILRDGRPIGTLNLYFADRGAFD
                     EQLTDLAQRMVSDVGFALAQIDREQARAEAERLAQERELQLSGLVESALDAIIAIDAR
                     QRVVLFNAAATRMFGVTAEAAIGGTLDRFIPPDERRAHRHAVDRYAAEGQTSRRMGLA
                     RELTGLRANGDIFPMEASISRSGEGERLLMTVMVRDVTHMRRAESERAARMLAEAANK
                     AKTEFLSRMSHELRTPLNAILGFSQLLRADARDPLSARHRERVELVIQAGEHLRSLIE
                     EMLDVSGIEAGRVAVVTRDFELREVLDGVLRMSAPHAAECGIRLEPLYAPDETMPVRG
                     DPSRVRQIVLNVVSNAIKYNRGGGRVQVGFERTPQHVRILVRDDGLGMTPAQLAQLFQ
                     PFNRLGREGGAAPGMGIGLVLVRQLARLLGGEVSVESASGQGTTVCVTLPAAAGPPAP
                     RDEDEAAGAVGDGHVAGRVLYIEDNPVNAMLVEQMLARWPRVEVTIAEDGESGLERAR
                     ATLPQVVLLDMQLPDISGLEVLRRLRAHPLTRDVPIIALSASGMPDEVHAARSAGADD
                     YWTKPVDLGAFLANMHELLTRRGAAPHGSH"
     gene            4634..>4821
                     /locus_tag="VF453_04060"
     CDS             4634..>4821
                     /locus_tag="VF453_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7686855.1"
                     /translation="MSIDTSAATAAPPSAAADERPGRYRRLLLPFDDSPASRHALDEA
                     LKLARESGASIELFAMFEA"
BASE COUNT          587 a         1740 c         1839 g          655 t
ORIGIN      
        1 cggacctgcc gctgcccatc gccaggcatc tcgcccgcgc gctggcgcac gccggcgagc
       61 cgccgttcgc ggttcgcctc gcgcatcgcg gcgagttcga cgtctcgccg cgtcgcgtcg
      121 cctggcgcgc gttcacgtcc cgccaatgga tgctggggtc gcgtccggga ttcgtctggg
      181 cggcgcgcat gtcgatgctg cctgccgtcg ccgtcgacgt cctcgatgcc tacgtggacg
      241 gcgtcgccga gttgcgggcc gacctgtggg ggtggctgcc cctgtcgaag gcgcgcgcca
      301 cgccggcgat cgcgcgcggc gagctgatgc gctggctcgc cgagtcggtg ctggtgccct
      361 gggcctggcg cacctgcccc ggcctgcgct ggcggcccgt ggacgaccgc catgcggacg
      421 cgacgctcgc cgacggcgcc gtcgacgtcg cgctgcactt cgcgttcggg gcggacggcc
      481 tcgccgaatc ggtgcacgcc gacagccgtc cccgcatggt cggcggcggc atcgtcgaga
      541 cgccctggaa cgggcgctgg tcggactacc gcacctgcga cggcgtgctg gtgccgaccc
      601 gcggcgaggt cgagtggcgc atcgacggcc ggcgcaagcc gtatttgcgc gcggcgctgg
      661 cgcgctacga aggcgtccag cgcgcgggcg aactgcacgc ctgacgcgcc ggcgcgtccg
      721 cggcttactg gatcgacagc agcttgctgg cggcgccggc ccgcttgggc agcgtcagcc
      781 gcagcacgcc gtcctcgagc ttggcggagg cggccttgtc gtcgacgtcc tgcgccagcg
      841 tgaacgtgcg cgaggtgcgt ccgcgccagg tctcgcgcag cagggcgcga ccgccgtcgg
      901 cgtgcttgtc ctcgacgtcc ttgcggacct cggcgctgat cgtgacctgg ttgcggtcga
      961 tctcgacgcg cacgtcgtcc ttgcgggcgc cggggacctc ggcgctcacg aggtattcct
     1021 tctcgttctc gctgacgtcg agccggatct ccgcgggcag gccggcgtcg aagctcagcg
     1081 gccgcgccag gcggcggaag aactcgggaa agaactcgtc catcggatcg aagcgggtga
     1141 gggcgctcat ggcgttctcc tgggtggttg gtaggcgatg ggactctagg aagcgccgcg
     1201 ccggatggac ttgagcgcgg tcaatgccct ccccgatcgg ccgcccggag cgccgccttg
     1261 cgctggcgca agccgatgcc cgcgccgggg cgaagaatcg tcgggccggg cagcgcccgc
     1321 cgacatccgg cgcggcggcc ggccccagga gagaacgcca tgatgaaaag cgatgcgcag
     1381 atcaagtccg acgtcgaggc cgagctcgcg tgggatccgc tggtcgacga ggcgcacgtc
     1441 ggcgtgagcg tgcgcgacgc cgtcgtcgag ctcaccggca cgctcggcag ccatgcggaa
     1501 cggctcgccg ccgagcgcgc agcgctgcgc gtcggcggcg tccagggcgt cacggtggcg
     1561 ctgcgcgtcg ccccgcccgc gggttgcgaa cgcgccgacg cggacgtcgt gaaggcctgc
     1621 cagcatgcgc tcgcgtggac ggccgacgtg cccgagggca tggtccggct tcgcgtcgag
     1681 aaggggcgcg tcacgctggt gggcgacgtg gactgggcct accagcgcca ggcggccgag
     1741 atggcggtct gcgacctgct gggcgtggtc gccgtcgtca acgagctccg tgtgcgcgcg
     1801 cggcccgcgg agacggacat ccgcgccggc atccgcgcgg cgctcgagcg ccaggccgac
     1861 cgcgacgcgg agcgcatcga cgtgcacgtg gacggctcct gcgtgatgct ctcgggccgc
     1921 gcgcgcacct gggccgagcg cgaggccgcg ctggccgccg cctggtcggc cgccggcgtc
     1981 acgcaggtgg tcaacgaggt cgtggtgcgg ccttgagagc cccgcccgac cggccgggct
     2041 cgccgccgga gtccatgacg atgtccctgc cgtccacgcc atgaccgcct cgccgccgtc
     2101 cgagctgccg tcgccctggc aggaactgct ggcgggtgcc ggactgatgg tcggcgtcgt
     2161 cgaccaggca ggccgtgtcc tgctcgccaa ccgggcgatg cgcgacgcgt gcggtcgagg
     2221 cgacgccggc gcgagcggcc tgcaatggcc gcgggacttc gtcgccaccg tcgaccaggc
     2281 agaggccgcc gaggcgttgg cgcgctggat cggcggcgat ccggccgctg gtgccgtcga
     2341 gctgtcccca ctcgagcgcg gtggtgcggc cgtgcgccgc gtgcgctggc atggtacgtc
     2401 gtggagcacc gccgcgggtg tggcgctgct gggcgtcgac gtctccgcgg agcgcgccca
     2461 ggaggccaag ctgctgcgca cgacgcgctt ctaccgcgcg ctgtccgaca tgaatgccgc
     2521 ggtggggcgg ctgcaggacg cgtcggcgct gtaccgggcc gtgtgcgaga tcgcggtcga
     2581 ctcgggccat gcgctgatgg cgtgggtcgg cctgcgcgac ggcgagttcc tggcgccggt
     2641 cgcctggggc ggcaacgccc gcgactacac gcgcggcctg gccctgcggc tggagggcgg
     2701 gccgcagccg cgcggtccct ccagcgacgc cttcgcgagc ggccgcccgt cgatctgcaa
     2761 cgacgtcgac accgatccgc ggatggcgcc ctggcgcgag catgcacggg ccttcggcgt
     2821 gcgttcgtcg gccgcgttcc cgatcctgcg tgatgggcgc ccgatcggca cgctcaacct
     2881 gtacttcgcc gaccgcggcg ccttcgacga gcaactgacc gacctcgcgc agcgcatggt
     2941 ctcggacgtc gggttcgcgc tcgcgcagat cgatcgcgag caggcgcgcg ccgaggccga
     3001 gcgcctggcg caggagcgcg agctgcagct gtccgggctc gtggaatcgg cgctggacgc
     3061 catcatcgcg atcgatgccc gccagcgcgt cgtgctgttc aacgccgcgg cgacgcgcat
     3121 gttcggcgtg acggccgagg cggccatcgg cggcacgctg gaccgcttca tcccgcccga
     3181 cgagcgccgc gcgcatcgcc acgcggtgga tcgctacgcg gccgagggcc agacgtcccg
     3241 ccgcatgggc ctggcccgcg agctcaccgg cctgcgcgcg aacggcgaca tcttcccgat
     3301 ggaggcctcc atctcgcgct cgggcgaagg cgagcggctg ctcatgacgg tgatggtgcg
     3361 cgacgtcacg cacatgcgcc gggccgagag cgagcgcgcc gcgcgcatgc tcgccgaggc
     3421 cgcgaacaag gcgaagaccg agttcctctc ccgcatgagc cacgagctgc gcacgccgct
     3481 gaacgcgatc ctcggcttct cgcagctgct gcgcgcggac gcgcgcgatc ccctgtcggc
     3541 gcggcaccgc gagcgcgtgg agctcgtgat ccaggccggc gagcacctgc gctcgctcat
     3601 cgaggagatg ctggacgtct ccggcatcga ggccggccgc gtggcggtcg tgacgcgcga
     3661 cttcgagctg cgggaggtgc tcgacggcgt gctgcgcatg agcgcgccgc atgcggccga
     3721 gtgcggcatc cgcctcgagc cgctgtacgc gccggacgag accatgcccg tgcgcggcga
     3781 tccgtcccgg gtgcggcaga tcgtgctcaa cgtggtctcc aacgcgatca agtacaaccg
     3841 cggcggcggg cgggtccagg tgggcttcga gcgcacgccg cagcacgtgc gcatcctcgt
     3901 ccgcgacgac ggcctgggga tgacgccggc gcagctcgcg cagctgttcc agccgttcaa
     3961 ccgcctcggg cgcgaaggcg gcgccgcgcc ggggatgggc atcggcctgg tgctcgtgcg
     4021 ccagctggcg cggctcctcg ggggcgaggt cagcgtggag agcgcgagcg ggcagggcac
     4081 gacggtgtgc gtcacgctgc ccgcggcggc gggcccgccg gcccctcgcg acgaggacga
     4141 ggcggcgggc gccgtgggcg acggccacgt ggcgggccgc gtgctctaca tcgaggacaa
     4201 cccggtcaac gcgatgctcg tcgagcagat gctcgcgcgc tggccgcgcg tggaggtgac
     4261 catcgcggag gacggcgaat cgggcctcga gcgggcccgc gcgaccctgc cgcaggtcgt
     4321 gctcctggac atgcagctgc ccgacatctc cggcctggag gtgctgcgcc ggctgcgcgc
     4381 gcatccgctc acgcgcgacg tgccgatcat cgcgctgtcc gcgagcggca tgcccgacga
     4441 ggtgcacgcg gcccgcagcg ccggcgccga cgactactgg acgaagccgg tcgacctcgg
     4501 cgccttcctc gcgaacatgc acgagctgct gactcggcgc ggtgcggccc cgcacggttc
     4561 gcattgacgg cgcgcaagcg cgcgcagccg cggccgccgc atgctggccg gcaggaatcc
     4621 acggagagag cgcatgtcca tcgacacttc ggccgcgacc gcggccccgc catcggccgc
     4681 ggcggacgag cgccccggcc gctaccgccg gctgctgctg ccgttcgacg acagcccggc
     4741 ctcgcggcat gcgctcgacg aggcgctgaa gctggcgcgc gagagcggcg cgtcgatcga
     4801 gctgttcgcg atgttcgagg c
//
LOCUS       DASVLN010000177         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_439264, whole genome shotgun sequence.
ACCESSION   DASVLN010000177 DASVLN010000000
VERSION     DASVLN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439264"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1016)
                     /locus_tag="VF453_04065"
     CDS             complement(42..1016)
                     /locus_tag="VF453_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX7686856.1"
                     /translation="MTVLWIVLALYLACIGVVHFRGRVRHSFWGQVFDHSGLLAPVNV
                     FLYAFSAVPNKPFIDLAHFRELDALAANSAVIREEGLALMELQRMKRPDQKDDAGFNS
                     FAKAGWKRFYLKWYGDSHPSAERLCPRTTELLRAIPSVKAAMFASLPPGGVLNPHKDP
                     YAGSMRYHLGLRTPNDDGCWIDVDGERYSWRDGQGVVFDETFIHTAENRTDVERLILF
                     CDVARPMRTRFADAFNRWFGRHVIAAASSPNDVGDRVGLVNRLFFLSHEAGIVRRRFK
                     AWNRTAYQVTKVALTLCLAVALLWAPSHRLLQALRGSPQAPAAAASGN"
     gene            1251..1616
                     /locus_tag="VF453_04070"
     CDS             1251..1616
                     /locus_tag="VF453_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7686857.1"
                     /translation="MLDWLDYRKQLLARIGEFGAASPDTVRGYQTLSNAGAKTGHLDA
                     KTRELIALAVAVTTRCDGCITVHSAQALKHGATKDEIAEALGVAVALNAGAAMVYSAR
                     VLDAVDAYGRAAPASAPAA"
     gene            complement(1782..>2292)
                     /locus_tag="VF453_04075"
     CDS             complement(1782..>2292)
                     /locus_tag="VF453_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686858.1"
                     /translation="DRFQVRSASEAADRWLAAVVGAPLRFVRLGDDAVGRPQAQRLHV
                     VTLESAAEVDALLRSRGLPPADPLRYRPNLVLAGRGAPLVPFVDDLLAAAEWPGGRIE
                     VQAACIRCVVPDVDPANGIAGHGVLDAMAELSAQRRPGGPVALGVYGRATPRARIGRG
                     QAMSLVFAF"
BASE COUNT          372 a          797 c          826 g          297 t
ORIGIN      
        1 ggagcgcgtc cgccgcagtc gtcgcaccgg ccgccgccgg ctcagttgcc ggacgcggcg
       61 gccgccggcg cctggggcga cccgcgcagc gcctgcagga gccggtgcga cggcgcccac
      121 agcagcgcga ctgccaggca cagcgtcagc gcgaccttgg tcacctggta ggccgtccgg
      181 ttccaggcct tgaagcggcg gcgcacgatg ccggcctcgt gcgacaggaa gaacagcctg
      241 ttcacgagcc cgacgcggtc gccgacgtcg ttgggcgagc tcgccgccgc gatcacgtgg
      301 cggccgaacc agcggttgaa ggcgtccgcg aagcgcgtgc gcatggggcg ggcgacgtcg
      361 cagaacagga tcagccgctc gacgtcggtc cggttctcgg ccgtgtggat gaaggtctcg
      421 tcgaagacca cgccctggcc gtcgcgccag ctgtagcgct cgccgtcgac gtcgatccag
      481 cagccgtcgt cgttcggggt gcgcaggccg aggtggtagc gcatcgagcc ggcgtaggga
      541 tccttgtgcg ggttgaggac gccgcccggc ggcagcgagg cgaacatcgc cgccttcacg
      601 ctcggaatcg cgcgcagcag ctcggtggtg cgcgggcaca ggcgctcggc cgacgggtgg
      661 gagtcgccgt accacttgag gtagaaccgc ttccagcccg ccttcgcgaa ggagttgaat
      721 ccggcgtcgt ccttctggtc gggccgcttc atgcgctgca gttccatcag cgccaggcct
      781 tcctcgcgga tcaccgccga attcgccgcc agcgcgtcga gctcgcggaa atgcgcgagg
      841 tcgatgaaag gcttgttcgg caccgcggag aacgcgtaca ggaagacgtt gaccggcgcg
      901 agcagcccgg agtggtcgaa gacctggccc cagaagctgt ggcgcacgcg gccgcggaag
      961 tgcacgacgc cgatgcaggc gaggtagagg gcgaggacga tccagagaac ggtcattgcg
     1021 caggaagggg agcgccgcgc gcccacggcc cggcatgcgg gcggcgggcg accgcggcga
     1081 agccgcaagg ataccggatc gtgctcctgc gcattcgccg gccgcgcccg gcgcaagacg
     1141 ttggagcaag cgatcaggac gccacggcat tgttcgtctg ctggcgctgc ggcacagtct
     1201 gcgccatggc agcgcacccc ggcgccgtcc ctacccacga aggaagcccc atgctcgact
     1261 ggctcgacta ccgcaagcaa ctcctcgccc gcatcggcga attcggcgcc gcctcgcccg
     1321 acacggtgcg gggctaccag acgctgtcca acgcgggcgc gaagaccgga cacctcgacg
     1381 ccaagacccg cgagctcatc gcgctggccg tcgcggtgac gacccgctgc gacggctgca
     1441 tcaccgtgca cagtgcgcag gccctcaagc acggcgcgac caaggacgag atcgccgagg
     1501 cgctgggcgt ggccgtggcg ctcaatgccg gcgccgcgat ggtgtactcg gcgcgcgtgc
     1561 tcgacgcggt cgacgcgtac ggccgggccg cgcccgcaag cgcgccggcg gcgtgaactt
     1621 caagtgaaat ctccaaacgc cgtataaccg gttgacttga aaagactgct gcggcggcgc
     1681 ccgctcgcgg gcagcagtcc agattagcta atgtaggtcg tgaggaatat catgtaagtc
     1741 actgtcgtac agcgatattt tgcgcaactt caagtgaacg ttcaaaacgc gaacacgagc
     1801 gacatcgcct ggccccggcc gatccgcgcc cggggcgtcg cccggccgta gacgcccagc
     1861 gccaccggtc cgccgggtcg ccgctgggcg ctcagctcgg ccatggcatc caacacgcca
     1921 tggccggcga tgccgttcgc ggggtcgaca tccggcacga cgcatcggat gcacgccgcc
     1981 tgcacttcga tgcgaccgcc gggccactcg gcggcggcga gcaggtcgtc gacgaagggc
     2041 accagcggcg cgccgcggcc ggcgaggacg aggttgggcc gatagcgaag cggatcggcc
     2101 ggcggcaggc cgcggctgcg gagcagcgcg tcgacttcgg ctgcggattc cagcgtcacc
     2161 acatgcagcc gctgcgcctg cggccggccg accgcgtcgt cgccaaggcg cacgaagcgc
     2221 agcggcgcgc cgacaacggc ggccagccag cggtcggccg cctcgctcgc gctgcggacc
     2281 tggaagcggt cg
//
LOCUS       DASVLN010000178         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_384661, whole genome shotgun sequence.
ACCESSION   DASVLN010000178 DASVLN010000000
VERSION     DASVLN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384661"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VF453_04080"
     CDS             complement(<1..597)
                     /locus_tag="VF453_04080"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7686859.1"
                     /translation="MKLDTTLHAETPEGIDILLRPAGMAPRVLAYSIDWMIRLVVAMM
                     GMAAMQALGGIGLGLSLVLAFGLEWLYPIVFELLPGAATPGKRVLGLQVMMDDGLPIT
                     PAASVTRNLLRAFDFLPFGFGLGVLSMLVRRDFKRVGDVVAGSLVVHVGQVKLAGALP
                     EVAPMAPRVPLQRPQQIALLRLAGRSGRITPERLDELAA"
     gene            complement(656..1219)
                     /locus_tag="VF453_04085"
     CDS             complement(656..1219)
                     /locus_tag="VF453_04085"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7686860.1"
                     /translation="MSDITTQNPFAPPLARLSEPSPAGGLVEASRGSRFAAALLDGVI
                     PGGVGLVLGVSAAMAIPGLGRESGQAIGAALGFAALFGVLIIGWLVVTGVMVYRYGQT
                     IGKRIMSIRVVRTDGSRAAFGRILGLRVGVMMVLGFIPLLGPLIGLIDSLLIFRDSSK
                     CLHDDIADTRVVTAASSEHATLAGSLA"
     gene            complement(1320..1868)
                     /locus_tag="VF453_04090"
     CDS             complement(1320..1868)
                     /locus_tag="VF453_04090"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7686861.1"
                     /translation="MSDHASQDDNRYAPPRAVVDEPLPVEGQLEPASRGQRFGGAFID
                     GLVAMAISIPVLIAMYGTQFFVAARGGPTALLGGVAVYMVLYLALQSWFLYTAGQTVG
                     KKAMGIRIVRADGGRAEFPRLLFLRQGLPMVINMIPFLGKLLTLVDVLFIFGASRRCV
                     HDYIADTVVVSAASSRHAVLQR"
     gene            complement(2015..2992)
                     /locus_tag="VF453_04095"
     CDS             complement(2015..2992)
                     /locus_tag="VF453_04095"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEX7686862.1"
                     /translation="MEIFDYDNILLLPRKCRVDSRSECDTSMALGGRRFKLPVVPANM
                     KTVLDETIARWLAANGYFYVMHRFDLDTVAFAKAMREAGLIVSISAGVKPADYAIVDR
                     LAAEGVGADYVTIDIAHGHAESVRRMIAHLKEKLPQAFVIAGNVGTPEAVIDLETWGA
                     DATKVGVGPGKVCITRLKTGFGTGGWQLSALKWCARVANKPIIADGGIRHHGDIAKSI
                     RFGATMVMIGSLFAGHEESPGQTVEVDGKLYKEYYGSASDFNKGEYRHVEGKRILEPI
                     KGTLAETLREMQEDLQSSISYAGGSRVEDIRRVNYVILGGENAGEHLFM"
     gene            complement(3011..>3471)
                     /locus_tag="VF453_04100"
     CDS             complement(3011..>3471)
                     /locus_tag="VF453_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686863.1"
                     /translation="IPESAERLARLGPYLPERLQAQPAAFDTSTPLPPHRADQRFWVH
                     SSLPHEGYFGVQMHASGTWPEGSTGWIALYEQIPAGVRDTRIARRLVRVVVGPLKLPD
                     APGKAHAVAPLYGLRWPLNAHVEKLVAAAWIEDAHGRITQLATDRCADPR"
BASE COUNT          506 a         1190 c         1290 g          485 t
ORIGIN      
        1 cgcggcgagc tcgtcgagcc gctcgggcgt gatgcggccc gagcggccgg ccaggcgcag
       61 cagggcgatc tgctgcggcc gctgcagcgg cacgcgcggg gccatcggcg cgacttccgg
      121 cagcgcgccg gcgagcttca cctgcccgac gtgcacgacc agcgagcccg cgacgacgtc
      181 gcccacgcgc ttgaagtcgc gccgcaccag catcgacagc acgcccaggc cgaagccgaa
      241 gggcaggaag tcgaaggcgc gcagcaggtt gcgcgtcacg gaggcggccg gcgtgatggg
      301 caggccgtcg tccatcatca cctgcaggcc cagcacgcgc ttgccgggcg tcgccgcgcc
      361 gggcaggagc tcgaaaacga tcggatagag ccactcgagc ccgaacgcga gcaccagcga
      421 cagccccagc ccgatgccgc ccagcgcctg catggcggcc atgcccatca tcgcgaccac
      481 caggcggatc atccagtcga tggaataggc gagcacccgc ggcgccatgc cggcggggcg
      541 cagcaggatg tcgatgcctt cgggcgtctc ggcgtggagg gtggtgtcga gcttcaagga
      601 cggcgggcgg cgcgccgccg gggcgtgcgc gcgcgtcggg cgggaggagg agcggtcagg
      661 cgaggctgcc ggccagcgtt gcgtgctccg aggaggcggc cgtgacgacg cgggtgtcgg
      721 cgatgtcgtc gtgcaggcac ttgctcgaat cgcggaagat cagcaggctg tcgatcagtc
      781 cgatcagggg cccgagcagc gggatgaagc cgagcaccat catcacgccc acgcgcagcc
      841 cgaggatgcg gccgaaggcg gcgcggctgc cgtcggtgcg cacgacgcgg atgctcatga
      901 tgcgcttgcc gatcgtctgg ccgtagcggt agaccatcac gccggtgacc acgagccagc
      961 cgatgatcag cacgccgaac agcgcggcga agccgagcgc ggccccgatc gcctggcccg
     1021 actcgcggcc cagcccggga atggccatcg cggcggacac gcccagcaca aggccgacgc
     1081 cgccagggat gacgccgtcc agcagggccg ccgcgaagcg cgacccgcgg ctcgcctcga
     1141 cgaggcctcc ggcgggcgag ggctcgctca ggcgggccag cggcggcgcg aagggattct
     1201 gggtggtgat gtcggacatg tcttcctctt tgcttggcta tcgggggagc gtcggacgcg
     1261 ccctctctct gtggccggtc gcgatcgccc gcgggcgccg tgccggggaa actcggggat
     1321 cagcgctgca gcaccgcgtg gcgcgacgac gcggcgctca cgaccaccgt gtcggcgatg
     1381 tagtcgtgca cgcagcggcg cgaggcgccg aagatgaaca gcacgtcgac cagcgtgagc
     1441 agcttgccca ggaacgggat catgttgatc accatcggca ggccctggcg caggaacagc
     1501 aggcgcggga actccgcccg gccaccgtcg gcgcgcacga tgcggatgcc catcgccttc
     1561 ttgccgacgg tctggccggc ggtgtagagg aaccacgact gcagcgccag atacagcacc
     1621 atgtagacgg ccacgccgcc cagcagcgcg gtcgggccgc cgcgggccgc cacgaagaac
     1681 tgcgtgccgt acatcgcgat cagcaccggg atgctgatcg ccatcgcgac gaggccgtcg
     1741 atgaaggcgc cgccgaagcg ctgcccgcgc gaggcgggct cgagctggcc ctcgacgggc
     1801 aagggttcgt ccacgaccgc gcgcgggggc gcatagcggt tgtcgtcctg gctggcgtga
     1861 tcgctcaaga gctgtcctcc ctggagggcg gcggagggcc gcggatgcaa gacgtctggc
     1921 gcgtcttcct gcgtgggggc gatcttaacc gcgctgcggg cggctggaat cggggtcgaa
     1981 ccggggatgg acggcgcgcg cggcgcgtgt cacgtcacat gaacaggtgc tcgccggcgt
     2041 tctcgccgcc caggatcacg tagttcacgc gccggatgtc ctcgacccgg ctgccgccgg
     2101 cgtaggagat cgagctctgc aggtcctcct gcatctcgcg cagggtctcg gccagcgtgc
     2161 ccttgatcgg ctcgaggatg cgcttgccct cgacgtggcg gtactcgccc ttgttgaagt
     2221 cggaggcgct gccgtagtac tccttgtaca gcttgccgtc gacctccacc gtctgccccg
     2281 ggctctcctc gtggccggcg aacagcgagc cgatcatcac catcgtcgcg ccgaagcgga
     2341 tgctcttggc gatgtcgccg tggtgccgga tgccgccgtc cgcgatgatg ggcttgttgg
     2401 ccacgcgggc gcaccacttg agcgccgaca gctgccagcc gcctgtgccg aagccggtct
     2461 tcagccgcgt gatgcagacc ttgcccgggc cgacgcccac cttggtcgcg tcggcgcccc
     2521 aggtctcgag gtcgatcacc gcctcgggcg tgcccacgtt gccggcgatc acgaaggcct
     2581 gcggcagctt ctccttcagg tgcgcgatca tgcgccgaac gctttcggcg tggccgtggg
     2641 cgatgtcgat cgtcacgtag tcggcgccca cgccctcggc ggcgaggcgg tcgacgatgg
     2701 cgtagtcggc cggcttgacc cccgcgctga tcgagacgat gagcccggcc tcgcgcatcg
     2761 ccttcgcgaa cgcgacggtg tcgaggtcga agcggtgcat gacgtagaag tagccgttgg
     2821 cggcgagcca gcgcgcgatc gtctcgtcca gcaccgtctt catgttggcc ggcaccaccg
     2881 gcagcttgaa gcggcggccg cccagggcca tcgaggtgtc gcattcgctg cggctgtcga
     2941 cgcggcactt gcgcggcagc agcaggatgt tgtcgtagtc gaagatttcc acggtcggtt
     3001 ccttgccgcg tcagcggggg tcggcgcagc ggtcggtggc gagctgggtg atgcgcccgt
     3061 gggcgtcctc gatccaggcc gcggcgacca gcttctccac gtgcgcgttg agcggccagc
     3121 gcaggccgta cagcggcgcg acggcgtggg ccttgcccgg cgcgtccggc agcttcagcg
     3181 ggccgaccac cacgcgcacc aggcggcgcg cgatcctcgt gtcgcgcacg ccggcgggga
     3241 tctgctcgta gagcgcgatc cagccggtcg agccctcggg ccaggtgccg ctcgcatgca
     3301 tctggacgcc gaagtagccc tcgtgcggca ggctggagtg cacccagaag cgctggtcgg
     3361 cgcgatgcgg gggcaggggc gtcgaggtgt cgaaggccgc gggctgcgcc tgcagccgct
     3421 cgggcaggta gggcccgagc cgcgcgaggc gctcggcgct ctcggggatc g
//
LOCUS       DASVLN010000179         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_331389, whole genome shotgun sequence.
ACCESSION   DASVLN010000179 DASVLN010000000
VERSION     DASVLN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331389"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1712
                     /gene="argH"
                     /locus_tag="VF453_04105"
     CDS             279..1712
                     /gene="argH"
                     /locus_tag="VF453_04105"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX7686864.1"
                     /translation="MSDNQLDKKSQAWSALFSEPMSELVKRYTASVGFDKRLWQADIA
                     GSLAHARMLAAQGVIAQQDFADIERGMAQIRADIEAGRFEWKLELEDVHLNIEARLTQ
                     LVGDAGKRLHTGRSRNDQVATDVRLWLRGEIDEIALLLADMQRALLAVAARHTETVMP
                     GYTHLQVAQPVSFAHHLLAYVEMFARDAERLADARKRVNRLPLGAAALAGTSYPLDRE
                     AVARALGFDGVCQNSLDAVSDRDFAIEFSAFASIVMVHVSRLAEEIVLWMNQNFGYVD
                     LSDRYCTGSSIMPQKRNPDVAELARGKSGRVVGHLMALITLMKGQPLTYNKDNQEDKE
                     PLFDTVDTVKDTLRIMAEMVGGELDAATGARSGGLVPKPEAMERAARRGYATATDLAD
                     YLVRKGVPFRDAHEVVAHAVKVGIERGVDLADLPLDVLQGFHAAIGEDVYGVLTLRGS
                     LEARRVTGGTAPVEAQRQIERHRARLG"
     gene            complement(1742..>2267)
                     /locus_tag="VF453_04110"
     CDS             complement(1742..>2267)
                     /locus_tag="VF453_04110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7686865.1"
                     /translation="ALPQVEALAGAAEAIPLPDASVDAVVCAQAFHWFATRAALAEMH
                     RVLVPGGRLGLVWNRRDARVPWVARLDAIVNRREGDVPRLHTGAWRRAFPFEGFGPLA
                     ESRFAHGHTGAPEDVIVNRVRSTSFIAGLPEGERARVDDEVRAMVASEPALAGRAEVT
                     VPYETEAYVAVRID"
BASE COUNT          335 a          837 c          819 g          276 t
ORIGIN      
        1 ccgcagcagg atgcccggct cgcacagcgc gggcacccgc gcctcggccg ggcgcgcggg
       61 cagcggctcg ccgagcacgg tcgtcaccgg gggttgggcg gacgcgggtt ccatcggccc
      121 gcattgtggg ggcgcgtgcg cgggcgccac aagccgcgcc atccgggcgg cgtgcgtggt
      181 cggacagccg gccgccgacg cctacaatcc gggttctccc tgaaccgccc tgcgccgatc
      241 ccgcgacatc ggtcgggccc tgccgcgcga aagacgagat gagcgacaac cagttggaca
      301 agaaatcgca ggcctggtcg gccctgttct ccgagccgat gagcgagctc gtcaagcgct
      361 acaccgccag cgtcggcttc gacaagcgcc tgtggcaggc cgacatcgcc ggcagcctcg
      421 cgcatgcgcg gatgctcgcc gcgcagggcg tgatcgcgca gcaggacttc gccgacatcg
      481 aacgcggcat ggcgcagatc cgcgccgaca tcgaggccgg ccgcttcgag tggaagctcg
      541 agctggagga cgtgcacctg aacatcgagg cacgcctgac gcagctcgtg ggcgacgccg
      601 gcaagcgcct gcacaccggg cgctcgcgca acgaccaggt cgcgaccgac gtgcgcctgt
      661 ggctgcgcgg cgagatcgac gagatcgccc tgctgctggc cgacatgcag cgcgcgctgc
      721 tggcggtcgc cgcgcgccac accgagaccg tgatgccggg ctacacgcac ctgcaggtcg
      781 cgcagccggt gagcttcgcg caccacctgc tcgcctacgt cgagatgttc gcgcgcgacg
      841 ccgagcgcct cgccgacgcc aggaagcgcg tgaaccgcct gccgctgggc gccgccgcgc
      901 tcgcgggcac cagctacccg ctcgaccgcg aggcggtcgc gcgcgcgctc ggcttcgacg
      961 gcgtgtgcca gaactcgctg gacgccgtga gcgaccgcga cttcgcgatc gagttctcgg
     1021 ccttcgcgtc catcgtcatg gtgcacgtct cgcgcctggc cgaggagatc gtgctctgga
     1081 tgaaccagaa cttcggctac gtcgacctgt cggaccgcta ctgcacgggc tcgtcgatca
     1141 tgccgcagaa gcgcaacccg gacgtcgccg agctggcgcg cggcaagagc ggccgcgtgg
     1201 tcggccacct gatggcgctg atcaccctga tgaagggcca gccgctcacc tacaacaagg
     1261 acaaccagga agacaaggag ccgctgttcg acaccgtcga cacggtcaag gacacgctgc
     1321 gcatcatggc cgagatggtg ggcggcgagc tcgacgccgc caccggcgcg cgctccggcg
     1381 gcctggtgcc caagcccgag gcgatggaac gcgccgcgcg ccgcggctac gccaccgcca
     1441 ccgacctcgc cgactacctg gtgaggaagg gcgtgccctt ccgcgatgcc cacgaggtcg
     1501 tggcgcacgc ggtcaaggtc ggcatcgagc gcggcgtcga cctcgccgac ctgccgctgg
     1561 acgtgctgca gggcttccac gcggccatcg gcgaggatgt ctacggcgtg ctcacgctgc
     1621 gcggctcgct cgaggcgcgg cgcgtcacgg gcgggacggc gcccgtggag gcgcagcggc
     1681 agatcgagcg gcaccgggca cgcctcggtt gatccgccgc ccgcgcgcgc gggtgcgagg
     1741 ctcaatcgat ccgcaccgcc acgtaggcct cggtctcgta cggcaccgtc acctcggcgc
     1801 ggcccgccag cgcaggctcc gacgccacca tggcgcgcac ctcgtcgtcg acgcgtgcgc
     1861 gctcgccctc gggcaggccg gcgatgaagc tggtcgagcg cacgcggttg acgatcacgt
     1921 cctccggagc gccggtatgg ccgtgcgcaa agcgcgactc ggcgagcggg ccgaagccct
     1981 cgaacgggaa cgcgcggcgc cacgcgccgg tgtgcaagcg cgggacgtcg ccctcgcggc
     2041 ggttgacgat cgcgtccagg cgcgcgaccc agggcacgcg ggcgtcgcgg cggttccaga
     2101 ccagccccag ccggccgccc ggcacgagca ctcggtgcat ctcggcgagc gcggcgcgcg
     2161 tcgcgaacca gtggaaggcc tgggcgcaga cgacggcgtc cacgctcgcg tcgggcagcg
     2221 ggatcgcctc ggcggcgccc gcgagtgcct cgacctgcgg cagcgcc
//
LOCUS       DASVLN010000180         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_278071, whole genome shotgun sequence.
ACCESSION   DASVLN010000180 DASVLN010000000
VERSION     DASVLN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278071"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..724)
                     /gene="rpiA"
                     /locus_tag="VF453_04115"
     CDS             complement(50..724)
                     /gene="rpiA"
                     /locus_tag="VF453_04115"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX7686866.1"
                     /translation="MTQDELKALVGQAAIAYVQPGSIVGVGTGSTVNHFIEALAVMPR
                     RVSGAVSSSVQSTERLVRHGIPVLDAGEVTGLGVYVDGADEIDASGCMIKGGGAALTR
                     EKIVADLAERFVCIADESKRVPVLGRFPLPVEVIPMAVRQVIRRFAALGGEATVRTGV
                     TTDNGNPIVDVRGLQIADPVAMESEVNQWPGIVCVGIFARHRADVCLLGTSTGVQTLT
                     YGSARG"
     gene            complement(733..1254)
                     /locus_tag="VF453_04120"
     CDS             complement(733..1254)
                     /locus_tag="VF453_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7686867.1"
                     /translation="MSRVHLVEGPVGAGKSTYSATLAATSGGVHIPLDEWFATLFSPD
                     RPDGDFVPWYLERKERLLGLIWSHSRRILSAGRSVVLELGLIQREQRIQFCRRVANEG
                     FPLHVHVLDASLEVRRGRVRRRNEERGPTFSMVVPDHVFEMASRLWEPPEELEREEFE
                     ISFVTDHPHRPRR"
     gene            complement(1355..>1547)
                     /locus_tag="VF453_04125"
     CDS             complement(1355..>1547)
                     /locus_tag="VF453_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686868.1"
                     /translation="MTKTIASQAVAAVLAVAATFATASGVTHIASQQFAMAEKAAMSQ
                     TGEQVASVQHVVVVARRLA"
BASE COUNT          243 a          528 c          538 g          238 t
ORIGIN      
        1 tcggcgcgac gctgctggcg gccgcgagcg cgatcgcgcc gcgcgaggat cagccgcgcg
       61 cggacccgta ggtcagcgtc tggacgccgg tggacgtgcc cagcaggcaa acgtcggcgc
      121 ggtggcgcgc gaagatgccc acgcacacga tgcccggcca ctggttgacc tcggactcca
      181 tcgccacggg gtcggcgatc tgcaggccgc gcacgtcgac gatcgggttg ccgttgtcgg
      241 tggtcacgcc cgtgcgcacg gtggcctcgc cgccgagcgc cgcgaaccgg cggatcacct
      301 gccggacggc catcgggatc acctcgaccg gcagcgggaa tcggccgagc accggcacgc
      361 gcttcgattc gtcggcgatg cacacgaagc gctcggccag gtcggcgacg atcttctcgc
      421 gcgtcagcgc cgcgccaccg cccttgatca tgcagccgct tgcgtcgatc tcgtccgcgc
      481 cgtcgacgta cacgcccagg cccgtcacct cgcccgcgtc gagcacgggg atgccgtggc
      541 gcaccaggcg ttcggtgctc tgcacggagc tcgacaccgc gcccgagacg cgccgaggca
      601 tgacagccag ggcctcgatg aagtggttga cggtcgagcc ggtgccgacg ccgacgatgc
      661 tgccgggttg cacgtaggcg atggccgcct ggccgacgag ggccttgagt tcgtcttgcg
      721 tcatggcagg gattatcgcc gcgggcggtg ggggtggtcc gtgacgaagc tgatctcgaa
      781 ctcttcgcgc tccagctcct ccggcggttc ccaaagcctg cttgccatct cgaacacatg
      841 gtccggcacg accatcgaaa acgtcgggcc gcgctcttcg ttcctgcgtc gaacacgccc
      901 gcgacgaact tccaaggaag cgtcgaggac atggacgtga agtggaaacc cttcgttcgc
      961 gaccctccgg cagaactgga ttcgctgctc ccgctggatc agacccagct cgagcacgac
     1021 gcttcgaccc gcggacaata tcctccggct gtggctccag atcaggccca gcaagcgctc
     1081 cttgcgttca aggtaccagg gcacgaaatc gccgtccggg cggtccgggc tgaaaagcgt
     1141 tgcgaaccat tcgtcgagcg ggatgtgcac gccgccggag gtcgcggcca aggtcgcgct
     1201 gtaggtggac tttccggccc cgaccgggcc ctcgaccaga tgaactcgag acatgcttga
     1261 cgatccagga tggggacagt ggccggaatt ctgacgcgat ctctgccatg aaaaagcccc
     1321 gcgctcggcg gggctcggcg gcggcgggcg tcgctcaggc gagacggcgc gcgaccacga
     1381 ccacgtgctg cacgctcgcg acctgctcgc cggtctgcga catcgcggcc ttctcggcca
     1441 tcgcgaactg ctggctggcg atgtgggtca ctccggacgc ggtggcgaag gtggcggcga
     1501 cggcgaggac ggcggcgacg gcttgcgagg cgatggtctt ggtcatg
//
LOCUS       DASVLN010000181        10053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_62311, whole genome shotgun sequence.
ACCESSION   DASVLN010000181 DASVLN010000000
VERSION     DASVLN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62311"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(473..1783)
                     /locus_tag="VF453_04130"
     CDS             complement(473..1783)
                     /locus_tag="VF453_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686869.1"
                     /translation="MANPDATTLPGPDFIDTLPPAPHVIPAPASRPHSDAPDRMLFYF
                     SGFDPKGATFYHRLFRNGIGQRNASHDETLAVGGRHRVGRWASVWTALWRGAPAPGGG
                     RPPTMRTRVHFMRWDDLVRRHWRRTPGQLARDYWNVYVRGAVSGVLWRVRRQAPSAFG
                     LAMLPLGVALVSLMAGLLVGSALEWIGALSPLASALVGLIMGAVLWRGLARWVDCEWL
                     LRLYAFVRSQALGQTPEVDERLDEMAGHVVEAVEARLRQPGAGPLREIMFVGHSTGTL
                     LASQVLARALGQLGEMVDSRRNRSGMTLSLVTLGHCLPIAAEWPGAQRLRGQLDVLSE
                     CPSLTWHDYSASSDRAAFWRAPPWPVPARLRGHQASPRFRGRLSAAAYAQLRRDERDA
                     HLQYLRPPVAKGDADTFDFFALTCGPATLAERHSSLKSAADLRD"
     gene            complement(1790..2785)
                     /locus_tag="VF453_04135"
     CDS             complement(1790..2785)
                     /locus_tag="VF453_04135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7686870.1"
                     /translation="MPATVTSPSADASEVGLRAPSLSARQLGLRVIERGWLSANQVVF
                     AAVDEAPATVVDTGFSLHAVQTLALVDHALEGAPLSRILNTHLHSDHCGGNHALQGRG
                     GVETWVPAASVDIVRRWDQEALTYVLTDQPCSRFAVDRALVPGGAISIGAAEWQIHAA
                     PGHDMDAILLFEPQTRTLIAGDALWESRLAIIFPELAGEDGFGPTRRTLDLIERLQPR
                     EVIPGHGRPFEDVEAALAASRERVAAFERQPERHAQHAARALLMFHLLEVREAAFEAL
                     VDWMYGTPIYRQVAARLGLDETRATAAARAHVERLVDDGVLERRGDRVGVRPQAS"
     gene            complement(2814..4502)
                     /locus_tag="VF453_04140"
     CDS             complement(2814..4502)
                     /locus_tag="VF453_04140"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX7686871.1"
                     /translation="MPRPWLAHYPPGVPATVPVDGYPSLVALLEEAWKKHAARDACVF
                     RGSRLTFAQVDERSLALAAWLQAKGLQCGDRVALMMPNLPQYMVAIGAVLRAGGVVVN
                     VNPLYTPRELHHQLADSGARFILVLENFAHVLEQALPGTDLRHVVLAAAGDLLGFAEG
                     QLVNFVARHVKKMVPEFRLPRDQGRSVTRFNMAVAEGTRLVLKPVPLGPDDVAFLQYT
                     GGTTGISKGATLLHRNVVANILQSEAWFRPMLETLGDRPLTAVCALPLYHIYALTICY
                     MFGARLGMKLLLIANPRDIPGFVKSLSKHRVNMFPAVNTLFNALANDADFAKLDFSDL
                     VVSNGGGMAVQQATAERWLRITGCPVVEGYGLSETSPVATSNRLDKPEFTGTIGLPIP
                     STDIAIRDDDGRDLPFGEPGEICIRGPQVMAGYWKRPDETAKVITPDGFFRSGDIGVM
                     DERGYIRIVDRKKDMILVSGFNVYPNEIEQVVNLHPGVLECAAVGIQDEKSGEAVKLF
                     VVRRDPALAEADIAAYCRENFTAYKRPRVIEFRDDLPKSNVGKILRRELRTPGA"
     gene            complement(4649..5377)
                     /locus_tag="VF453_04145"
     CDS             complement(4649..5377)
                     /locus_tag="VF453_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686872.1"
                     /translation="MSDPASRILAALAAVESERARRAAAPGFAATVESLKSWQQRRFA
                     RTHADLLEHPRFGRAARFFLDELYGPQDFARRDAEFGRIVPAVVRLFPEDIVLTVEAL
                     AEVHALSERLDGAMAARLAGPTIDGPAYVRAWQAVGEPQAREHQIELAMVVGRALERH
                     VRSRVLRASLKAMRGPARAAGLGTLQAFLETGFDAFAAMRGADEFLGTIERRERALGA
                     RLFEPTAEQAAAAPAADDPLAALP"
     gene            5476..6411
                     /locus_tag="VF453_04150"
     CDS             5476..6411
                     /locus_tag="VF453_04150"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX7686873.1"
                     /translation="MVASIARLLFIAWFALLAALLAWGAQSGWPWGAWLGLYVLVLGH
                     PTLLAIELAAARIVSRRAQAGAPGPAPLPVGAMRLLRAWLREWLVSTIVFGVRLPWRA
                     QAWPDHLPRSSRGLRGVVFVHGFLCNRGMWNPWLKRLTRLERAFVAVDLEPVHSDIDA
                     YAELVERAVRRIEAVTGLAPVIVAHSMGGLAVRAWLRTRAGRAESHGHEHAARVITIG
                     TPHHGSRVARLSRAANAVQMRVGSEWLAALARQDGADHGALFTCWWSDCDQLVYPPST
                     AVLADSESRQLRGVGHLAMCGREEIWEEIRRRIGR"
     gene            complement(6428..7540)
                     /gene="hppD"
                     /locus_tag="VF453_04155"
     CDS             complement(6428..7540)
                     /gene="hppD"
                     /locus_tag="VF453_04155"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX7686874.1"
                     /translation="MAFTPWDNPMGTAGFEFIEYAAPDPAAMAAVFERMGFKAIARHR
                     HKAVTLYRQGHINFILNAEPDSFAQRFARLHGPSICAIAFRVQDAKFAYERAIGLGAW
                     GFAGAAGPGELNIPAIKGIGDSLIYLVDRWRGKNGAKPGDIGNIGFYDVDFEPLPGVS
                     REELDPQGNGLLEIDHLTHNVHRGRMEEWAEFYERLFNFREVRYFDIEGKVTGIRSKA
                     MTSPCGLIRIPINEEGNDGKGQVQEYLDRYHGEGIQHIALTSANLPDTVDRLRASGVK
                     LLSTPDTYYELVDKRIPGHGEDVKALHERQILVDGAPGELLLQIFSENQLGPIFFEFI
                     QRKGNEGFGEGNFKALFESIELDQIRRGVLKADLPA"
     gene            7680..8162
                     /locus_tag="VF453_04160"
     CDS             7680..8162
                     /locus_tag="VF453_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7686875.1"
                     /translation="MSAATDLDPIDRRILQALQADGRLTFDELAGRVGLSASAVLRRV
                     RRLEEAEVISGYVALVQPEAVGLGLTAYLNVRLEKHSEAHKRNPMDLFKAAVQTWPEV
                     VECVSLTGEMDYLLRVVVTDMAHYARFIGDTLLRHPSVQDCKSSFVLERIKGTTAMPV
                     "
     gene            complement(8171..8794)
                     /locus_tag="VF453_04165"
     CDS             complement(8171..8794)
                     /locus_tag="VF453_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7686876.1"
                     /translation="MPGPDRQFWQERFERNETPWDRGGAGPQLLAWVENGVLDATALA
                     GRIAVPGCGSGHDVLALAQRGFDVVGIDYAQAACERTRARLAAAGATAEVVAADVLAW
                     QPARPLAAVYEQTCLCAMHPDHWTRYARALHDWLRPGGRLFLMGMQVSRPAAADGVIE
                     GPPYNVPVNALRALFDGSRWEWPAPPYPRVAHPFADAYELGLVLVRR"
     gene            complement(8883..9731)
                     /locus_tag="VF453_04170"
     CDS             complement(8883..9731)
                     /locus_tag="VF453_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX7686877.1"
                     /translation="MNAPTGSSDSPAPRTVAGWLRALGDPEIPVLADTAAALEDMRLV
                     EDDVDARGIASAIGRDPLMTLKVLAYASTQGSNHRLTDAETVLEALVLMGITPFFRAF
                     GPQPSAEDRLEAHPQALAGLQRVLARADRAARFAAAFAVHRADHDAVVLHEAAQLHDF
                     AEMLLWLHAPALALEIRRRQAADPALRSAIVQRELLGAELNDIQHALMQQWRLPEILV
                     RITDDRHAENSQVRNVLLAIRVARHTMDGWDNPAVPDDVRDIATLLRLNEASALAFLH
                     EVDAQD"
BASE COUNT         1438 a         3504 c         3664 g         1447 t
ORIGIN      
        1 tccttcccat cccgaacagg acagtgaaac gcctctgcgc cgatgatagt gcggattccc
       61 gtgtgaaagt aggacattgt caggctaata tcccgcaacc gcccgagctg aaaccttcag
      121 ctcgggcgtt ttgctttgcg gagcccgcag ccgcccgagc tgaaaccttc ggcttgggcg
      181 ctttggcttt gagggcattg aatcggcgtc gcccctgggc tcctttcggg actggcctgt
      241 tatccgcggc ggcttcttgt cgtcgggcgt accggcgtgc tggtgccccg ccttccaggc
      301 gccatcctcg cgcctgacga tccgggcgac ccgaagggcc gtctccaccg tggccggctt
      361 gccgcggaca tccatcgtcg cccgctcgat tccggcgcag aagccgagat cgtcggatgt
      421 ccgggcgcag cacgttccgg ccgcagcgac cggatggcga acgcgtgaaa cctcagtcgc
      481 gcaggtccgc cgccgacttc agcgaggaat ggcgctcggc cagggtcgcg gggccgcacg
      541 tcagggcaaa gaagtcgaag gtgtcggcat cgcccttggc cacgggggga cgaaggtact
      601 gcaggtgcgc atcccgctcg tcgcggcgca gttgagcgta cgccgccgca gacagccggc
      661 cgcggaagcg cggcgacgcc tgatggcctc gcagccgcgc cggcaccggc cagggcgggg
      721 cgcgccagaa cgccgcgcgg tcggaggacg ccgaatagtc gtgccaggtc agcgacggac
      781 actccgacaa cacgtccagc tggcctcgaa gccgctgggc gcccggccac tcggcggcga
      841 tcggcaggca atgaccgagc gtcaccaacg acaaggtcat gccgctgcga ttgcgacggc
      901 tgtccaccat ctcgccgagc tggcccagcg cgcgggcgag tacctgcgac gcgagcaagg
      961 tgcccgtcga gtgcccgacg aacatgatct cccgcagcgg gcccgcgccg ggctggcgca
     1021 gccgcgcctc gaccgcctcg acgacgtggc cggccatctc gtccaggcgc tcgtcgacct
     1081 ccggtgtctg gccgagcgcc tgcgagcgca cgaaggcgta gagccgcaaa agccactcgc
     1141 aatcgaccca gcgagcgagg ccgcgccata gcacggcgcc catgatcagg ccgaccagcg
     1201 ccgaggccag cggcgagagt gcgccaatcc actccagcgc gctgccgacc agcagtcccg
     1261 ccatcagcga caccagcgcc acccccagcg gcagcatcgc gagcccgaac gccgacggcg
     1321 cctgccgtct cacgcgccac agcacacccg agacggcgcc gcgcacgtac acgttccagt
     1381 agtcgcgcgc cagctggccg ggcgtgcggc gccagtgccg gcgaaccagg tcatcccagc
     1441 gcatgaagtg cacgcgcgtg cgcatggtcg gcggacggcc tccgcctggt gccggagcgc
     1501 cgcgccagag ggccgtccag accgaagccc agcgacccac gcggtgacgg ccaccgacgg
     1561 ccagcgtctc gtcgtgagag gcattgcgct ggccgatgcc gttgcggaat agccggtgat
     1621 agaacgtcgc gcccttggga tcgaagccgc tgaagtagaa cagcatgcga tccggcgcgt
     1681 cggaatgcgg gcgcgacgcg ggcgccggga tgacgtgcgg cgccggcggc agcgtgtcga
     1741 tgaaatccgg cccgggcagg gtcgtggcgt cgggattggc catgccgcct cagctcgcct
     1801 gcggccgcac gcccacgcga tcgccgcggc gctcgagcac gccgtcgtcg accaggcgct
     1861 ccacgtgcgc gcgcgcggcc gcggtcgcac gcgtctcgtc gagtcccagg cgcgcagcca
     1921 cctggcgata gatgggtgtg ccgtacatcc agtcgaccag cgcctcgaac gccgcttcgc
     1981 gcacctcgag caggtggaac atcagcaacg cgcgcgcggc atgctgggcg tgccgctcgg
     2041 gctgacgctc gaaggcggcc acgcgctctc ggctggcggc gagcgcggcc tcgacgtcct
     2101 cgaacgggcg gccgtggccg gggatgacct cgcgcggctg caggcgctcg atcaggtcga
     2161 gcgtgcggcg cgtcgggccg aagccgtcct cgccggcgag ctcggggaag atgatggcca
     2221 ggcgagactc ccacaacgcg tcgccggcga tcagcgtgcg cgtctgcggc tcgaacagca
     2281 ggatcgcgtc catgtcgtgg cccggcgccg catggatctg ccactcggcg gcaccgatcg
     2341 agatcgcccc gccgggcacg agcgcgcgat cgacggcgaa gcgggagcaa ggctggtccg
     2401 tgagcacgta ggtcaacgct tcctggtccc aacgccgcac gatgtccacg ctggcggcag
     2461 gcacccaggt ctcgacaccg ccgcggccct gcagcgcatg gttgccgccg cagtggtccg
     2521 agtgcaggtg ggtgttgagg atgcggctga gcggcgcgcc ttccagcgcg tggtcgacca
     2581 gcgccagcgt ctgcaccgca tgcaggctga agccggtgtc gaccaccgtg gccggggcct
     2641 cgtccacagc ggcgaagacg acctggttgg ccgacagcca gccgcgctcg atcacgcgca
     2701 gcccgagctg ccgggccgaa agcgagggcg cgcgcaagcc gacctcggag gcgtcggcgg
     2761 aagggctcgt gacggtcgcg ggcacggaaa cggaacggga aaaccgggca ggctcaggca
     2821 ccgggcgttc gcagctcgcg gcgcaggatc ttgccgacgt tggacttggg caggtcgtcc
     2881 cggaactcga tgacccgggg tcgcttgtag gccgtgaaat tctcgcggca atacgccgcg
     2941 atgtcggctt ccgcaagcgc cggatcgcgc cggaccacga acaacttcac ggcctcgccc
     3001 gacttctcgt cctggatccc caccgcggcg cactcgagca cgcccggatg caggttgacc
     3061 acctgctcga tctcgttcgg atacacgttg aacccggaca cgaggatcat gtccttcttg
     3121 cgatcgacga tgcggatgta gccgcgctcg tccatcacgc cgatgtcgcc ggagcggaag
     3181 aagccatccg gcgtgatgac cttggcggtc tcgtccggtc gtttccagta gccggccatc
     3241 acctgcgggc cgcggatgca gatctcgccc ggctcgccga acggcaggtc gcgaccgtcg
     3301 tcgtcgcgga tcgcgatgtc cgtcgacggg atgggcaggc cgatggtgcc cgtgaactcg
     3361 ggcttgtcca gccggttgct ggtggccacg ggcgaggtct ccgacaggcc gtagccctcg
     3421 acgacggggc agcccgtgat gcgcagccag cgctcggccg tcgcctgctg caccgccatg
     3481 ccgccgccgt tggagacgac gaggtcggag aagtccagct tggcgaagtc ggcatcgttg
     3541 gcgagcgcgt tgaacagcgt gttgaccgcc gggaacatgt tgacgcggtg cttgctcagc
     3601 gacttcacga agcccgggat gtcgcgcggg ttcgcgatca gcagcagctt cattcccagg
     3661 cgtgcaccga acatgtagca gatggtcagc gcgtagatgt ggtacagcgg cagcgcgcac
     3721 acggcggtca ggggcctgtc gccgagcgtc tcgagcatcg gccggaacca cgcctcgctc
     3781 tgcaggatgt tggcgaccac gttgcggtgc aggagcgtcg cgcccttgga gatgcccgtc
     3841 gtcccgccgg tgtactgcag gaacgcgacg tcgtccggcc ccagcggcac gggcttcagc
     3901 acgagccgcg tgccttcggc aacggccatg ttgaagcgcg tgaccgagcg accctggtcg
     3961 cgcggcaacc ggaactcagg caccatcttc ttgacgtggc gggccacgaa gttgaccagc
     4021 tggccttcgg cgaagcccag caggtcgccg gccgccgcga gcacgacgtg gcgcagatcg
     4081 gtgccgggca gcgcctgttc cagcacatgt gcgaagttct cgagcacgag gatgaagcgg
     4141 gcgccggagt cggcgagctg gtggtgcagc tctcgcggcg tgtacagcgg gttgacgttg
     4201 acgacgaccc cgcccgcgcg cagcacggcg ccgatggcga ccatgtactg cggcaggttg
     4261 ggcatcatca gcgcgacgcg gtcgccgcat tgcagcccct tggcctgcag ccacgccgcc
     4321 agcgccaggg aacgctcgtc gacctgcgcg aaggtcaggc gcgatccgcg gaagacgcag
     4381 gcgtcgcgcg cggcgtgctt cttccacgct tcctccagca gcgccaccag cgacggatag
     4441 ccgtcgaccg gcaccgtcgc gggcacgccg ggcgggtagt gcgcgagcca gggacggggc
     4501 aggtcgtcgg cgggcgccgc gagcggcagg gcggaggacg tgacggcgtt catgacgcgg
     4561 attgtcccgc agcccgccgg cacctccacc gaggggaaat cggcatttcc cgcgccgcgc
     4621 cgggcctggg cggcttccat gtggctcgct acggcagcgc ggccagcgga tcgtcggctg
     4681 ccggagcagc tgccgcctgt tcggcggtcg gctcgaacag gcgggcgccg agcgcgcgct
     4741 cgcgccgctc gatggtgccg aggaactcgt cggcgccgcg catcgccgcg aaggcgtcga
     4801 atccggtctc gaggaaggcc tgcagcgtgc cgaggccggc cgcgcgcgcc ggcccgcgca
     4861 tcgccttgag gctcgcgcgc aggacgcggc tgcgcacgtg gcgctcgagg gcgcgcccca
     4921 cgaccatcgc gagctcgatc tggtgctcgc gcgcctgcgg ctcgccgacg gcctgccacg
     4981 cgcgcacgta ggccggcccg tcgatcgtcg ggccggcgag gcgggcggcc atcgcgccgt
     5041 ccagccgctc ggacaacgcg tgcacctccg ccagcgcctc gaccgtgagc acgatgtcct
     5101 cgggaaacag ccgcacgacg gccggcacga tgcgtccgaa ctcggcgtcg cggcgggcga
     5161 agtcctgcgg gccgtagagt tcgtcgagga agaaccgcgc cgcgcgcccg aagcgcgggt
     5221 gctcgagcag gtcggcatgg gtgcgggcga agcggcgctg ctgccaggac ttcagcgact
     5281 cgaccgtcgc ggcgaatccg ggcgcggccg cccgccgcgc gcgctcggac tcgacggccg
     5341 cgagcgcggc gaggatgcgg gaagcgggat cgctcatcgc gcgcagggta tcaggccgcc
     5401 acggccattc gggcccgccg cgatgccgcg tatttcctgg acgggacacc aggcgcacgg
     5461 ggtcatactc ggcgcatggt cgccagcatc gcccgactgc tgttcatcgc ctggttcgcg
     5521 ctgctcgcgg cattgctggc ctggggcgcg cagtctggct ggccctgggg cgcatggctc
     5581 ggcctctacg tgctcgtgct ggggcacccg acgctgctcg cgatcgagct ggccgccgcg
     5641 cgcatcgtct cgcgccgcgc gcaggccggc gcgccgggcc ctgcgcccct gcccgtgggc
     5701 gcgatgcggc tgctgcgcgc gtggctgcgc gaatggctgg tctcgaccat cgtctttggc
     5761 gtgcggctgc cctggcgtgc gcaggcctgg ccggatcacc tgccgcgcag ctcgcgcggg
     5821 ctgcgcggcg tcgtcttcgt gcacggcttc ctctgcaacc gcggcatgtg gaatccgtgg
     5881 ctcaagcggc tgacgcgcct cgagcgcgcc ttcgtcgcgg tcgacctcga gccggtccat
     5941 tcggacatcg atgcctacgc cgagctcgtc gagcgcgccg tgcgccgcat cgaggcggtg
     6001 accggcctcg cacccgtgat cgtggcgcac agcatgggcg ggctcgcggt gcgcgcctgg
     6061 ctgcgcacgc gcgcgggccg cgccgagagc cacggccacg agcatgccgc ccgcgtcatc
     6121 acgatcggca cgccgcacca cggcagccgg gtcgcgcgcc tctcgcgcgc cgccaacgcg
     6181 gtgcagatgc gagtcggcag cgagtggctc gcggcgctcg cgcgccagga cggcgccgac
     6241 cacggcgcgc tgttcacatg ctggtggagc gactgcgacc agctggtcta tccgccctcg
     6301 accgccgtcc ttgcggacag cgaatcgcgc cagctgcgcg gcgtcggcca tctcgcgatg
     6361 tgcgggcgcg aggagatctg ggaagagatc cggcgccgca tcgggcgctg aggccggcgt
     6421 cgcgggctca ggccggcagg tcggccttca gcacgccgcg gcggatctgg tcgagctcga
     6481 tcgactcgaa cagcgccttg aagttgcctt cgccgaagcc ctcgttgccc ttgcgctgga
     6541 tgaactcgaa gaagatcggc ccgagctggt tttccgagaa gatctgcagc agcagctcgc
     6601 ccggcgcgcc gtcgaccagg atctggcgct cgtgcagcgc cttgacgtcc tcgccgtggc
     6661 cggggatgcg cttgtcgacc agctcgtaat aggtgtccgg cgtggacagc agcttgacgc
     6721 cgctggcacg cagccggtcg acggtgtcgg gcaggttggc cgaggtcagc gcgatgtgct
     6781 ggatgccttc gccgtggtac cggtcgaggt attcctgcac ctggcccttg ccgtcgttgc
     6841 cctcctcgtt gatcgggatg cggatcaggc cgcaggggct ggtcatcgcc ttgctgcgga
     6901 tgccggtgac cttgccctcg atgtcgaagt agcgcacctc gcggaagttg aacaggcgtt
     6961 cgtagaactc ggcccattcc tccatgcggc cgcggtgcac gttgtgcgtg aggtggtcga
     7021 tctccagcag gccgttgccc tgcgggtcga gttcttcgcg cgagacgccc ggcaggggct
     7081 cgaagtcgac gtcgtagaag ccgatgttgc cgatgtcgcc cggcttcgcg ccgttcttgc
     7141 cgcgccagcg gtcgaccagg tagatgagcg agtcgccgat gcccttgatc gccgggatgt
     7201 tgagctcgcc cgggccggcc gcgccggcga agccccaggc gcccaggccg atcgcgcgct
     7261 cgtaggcgaa cttggcgtcc tgcacgcgga aggcgatcgc gcagatgctg ggaccgtgca
     7321 ggcgtgcgaa gcgctgcgcg aacgagtccg gctcggcgtt gaggatgaag ttgatgtggc
     7381 cctggcggta gagcgtcacg gccttgtggc ggtggcgcgc gatcgccttg aagcccatgc
     7441 gctcgaagac cgcggccatc gcggcgggat cgggcgcggc gtactcgatg aactcgaagc
     7501 cggcggtgcc catcgggttg tcccaggggg tgaaggccat ggatgtctcc tgcgtggaat
     7561 ggggccgtgc gaaggctgcc ggcgcgatgg acgcatccta ggcccgcgtg ggcgcagttt
     7621 tcctgcgtat tccgtctcgg cattgccgga ttgcgcagga atcgtgtaca aatagggtca
     7681 tgagcgccgc caccgacctc gatcccatcg atcgacgcat ccttcaggcg ttgcaggcgg
     7741 acggccggct gaccttcgac gagctcgcgg gccgcgtcgg gctgtccgcc tcggcggtgc
     7801 tgcggcgagt gcgccggctc gaggaggccg aggtcatctc cggctacgtc gcgctggtgc
     7861 agccggaagc cgtcggcctc gggctgacgg cctacctgaa cgtgcggctc gagaagcaca
     7921 gcgaggccca caagcgcaac ccgatggatc tcttcaaggc tgcggtgcag acctggcccg
     7981 aggtcgtcga gtgcgtctcg ctcacgggcg agatggacta tctgctgcgc gtcgtcgtga
     8041 ccgacatggc gcactacgcg cgcttcatcg gcgacacgct gctgcgccac ccgagcgtgc
     8101 aggactgcaa gtccagcttc gtgctcgagc gcatcaaggg cacgacggcg atgcccgtct
     8161 gagcacgcct tcagcgccgc acgagcacga gtcccagctc gtaggcgtcc gcgaacgggt
     8221 gcgcgacgcg cggatacggc ggtgccggcc attcccaccg cgagccgtcg aacagcgccc
     8281 gcaaggcgtt gaccgggacg ttgtacggcg ggccctcgat cacgccgtcg gcggcggccg
     8341 gccgggagac ctgcatcccc atcaggaaca gccgcccgcc gggccgcagc cagtcgtgca
     8401 acgcccgcgc atagcgcgtc cagtggtcgg gatgcatggc gcacaggcag gtctgttcgt
     8461 agacggcggc caacgggcgg gccggctgcc acgcgaggac gtcggccgcg acgacctcgg
     8521 cggtcgcgcc cgctgccgcc agccgtgcgc gcgtgcgctc gcaggcggcc tgcgcgtaat
     8581 cgatgccgac gacgtcgaac ccgcgctggg cgagcgcgag cacgtcgtgg ccgctgccgc
     8641 agcccggcac cgcgatgcgg cccgccaggg ccgtcgcatc cagcacgccg ttctcgaccc
     8701 aggcgagcag ctgcggtccg gcgccgccgc gatcccacgg cgtctcgttg cgttcgaagc
     8761 gttcctgcca gaactggcga tcaggacccg gcatgggcat tcctcgtggc gtcgatgcgc
     8821 gatcgtagtg ccgtttcggc ggacgcgcgc gccgcgtccc tcctggtctg gcgtcgcgtg
     8881 actcagtcct gtgcgtcgac ctcgtgcagg aaggcgagcg ccgaggcctc gttgagccgc
     8941 agcagggtgg cgatgtcgcg tacgtcgtcg ggcacggcgg ggttgtccca gccgtccatg
     9001 gtgtggcgcg ccacccggat cgccagcagc acgtttcgca cctgcgagtt ctcggcgtgg
     9061 cggtcgtcgg tgatgcgcac gaggatctcg ggcagccgcc attgctgcat gagggcgtgc
     9121 tggatgtcgt tgagctccgc gcccagcagc tcgcgctgca cgatggccga gcgcagcgcc
     9181 gggtcggccg cctggcgccg gcggatctcg agcgccagcg ccggagcgtg cagccagagc
     9241 agcatctcgg cgaagtcgtg gagctgggcc gcctcgtgca ggacgacggc gtcgtggtcg
     9301 gcgcggtgca cggcgaatgc ggcggcgaag cgcgcggcgc ggtcggcgcg cgcgagcacg
     9361 cgctgcagtc cggccagtgc ctgcgggtgt gcttccaggc gatcctccgc cgagggctgc
     9421 ggcccgaacg cgcggaagaa gggcgtgatg cccatgagca cgagcgcctc gagcacggtc
     9481 tcggcgtcgg tgaggcgatg gttcgaaccc tgggtcgacg cgtaggcgag caccttcagc
     9541 gtcatcagcg ggtcgcgtcc gatcgccgag gcgatgccgc gcgcgtcgac atcgtcctcg
     9601 accaggcgca tgtcttcgag cgccgccgcg gtgtcggcga ggacggggat ctccgggtcg
     9661 cccagcgccc gcagccagcc ggccacggtg cgcggcgcgg gggagtcgga cgagccggtc
     9721 ggagcgttca tggcggagtg tcggcaggcg gcgggcccgc ggcgtgcgcg ggcctcccct
     9781 gtcttatcgg tgctgcgcgg gccggcttga ggccggcgtc ggcgccgcgc gcatcacgcg
     9841 cgtgcgccgc gtgcgggaat ccacgcccgc agccacacgc gcgtccacac ccgggtgcag
     9901 gcccgcgccc agacccgcgt ccaaaggcga tgggacatca cagccggcgc acgtccacgt
     9961 agccgacgag ccgaccgtcg acgtacaacg cgccttcggg cgcgccggca tcggcgtgga
    10021 actcgacggt gacgtcgtgc tggctggcgc ggc
//
LOCUS       DASVLN010000182         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_492790, whole genome shotgun sequence.
ACCESSION   DASVLN010000182 DASVLN010000000
VERSION     DASVLN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492790"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VF453_04175"
     CDS             complement(<1..222)
                     /locus_tag="VF453_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686878.1"
                     /translation="MTAEDADPTALLARLLAAARRKSADVAAELHPAWMERGLRRLGL
                     ADRAAAAADARWSATLAEVYDLRWPGLDRL"
     gene            complement(219..974)
                     /gene="sctJ"
                     /locus_tag="VF453_04180"
     CDS             complement(219..974)
                     /gene="sctJ"
                     /locus_tag="VF453_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004530627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HEX7686879.1"
                     /translation="MAGSIASRLARLALALGLACMLAACKQEVFTKLSEGDANDMLEV
                     LLGAHLDAAKTTADGKTWDVTVESSQLGTALATLHANGLPAEHRASLGDMFKKDGLIS
                     TPTEERVRFIYGVSQELERTLASIDGVVAARVHVVLPNNDPMADHVKPSSASVFIKYR
                     PDINVATIVPTVKNLVVRSVEGLTYENVNVTLVAAIVPAAPPVPAGEGDGSPVLAIAL
                     AAVLAAAGGGWMFWKRRNAAAAAAAPVAATGRP"
     gene            complement(982..1380)
                     /locus_tag="VF453_04185"
     CDS             complement(982..1380)
                     /locus_tag="VF453_04185"
                     /inference="COORDINATES: protein motif:HMM:NF021036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686880.1"
                     /translation="MTPVAPIPPIDPLASIGSAGAAGTPQAAAAPELRQRFESLMQRH
                     AQEAAADSTGKNATAIGGMIDREQSGLNELQSDMTSFIEHMPSMDATERTAGSMMLMD
                     KVSEAHIKMSLTMGVTKSSTKSLQSLLKNE"
     gene            complement(1415..1867)
                     /locus_tag="VF453_04190"
     CDS             complement(1415..1867)
                     /locus_tag="VF453_04190"
                     /inference="COORDINATES: protein motif:HMM:NF021153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HrpB1 family type III secretion system apparatus
                     protein"
                     /protein_id="HEX7686881.1"
                     /translation="MQNNALPVAQMRIIVAAHVLSDKFGVQADTGRILAVMAASVPDR
                     PEIVAAQARNLLIARQYPEARDLLTEAEAKHPRNAVIKALLAMCMFVQKDSLWEAYAE
                     EALALPRDAVALGIVEMLSRTSGRALRGLEARAEPSPPHHYAFVGLAC"
     gene            complement(1994..2356)
                     /locus_tag="VF453_04195"
     CDS             complement(1994..2356)
                     /locus_tag="VF453_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686882.1"
                     /translation="MPNANKVSHGATTAGQYMQMGKTGANTAVQSGVGSAVGGAAVTN
                     INDSTGDAVSNYLSQQNTALANQAAMASAAANMQNQTAAIDMAQQDNAAANHQAVAMD
                     NARNKLANTGADGMKDLA"
     gene            complement(2450..2722)
                     /locus_tag="VF453_04200"
     CDS             complement(2450..2722)
                     /locus_tag="VF453_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686883.1"
                     /translation="MTTAFTLPPDDLLAAFDSLEATLKTDTSGDRARRLNRYFAQVEL
                     EARQSQVRSIDFDEKRMAGLLAEAFAATQRGVCAAWNKLHGRELAA"
     gene            complement(2736..>3430)
                     /locus_tag="VF453_04205"
     CDS             complement(2736..>3430)
                     /locus_tag="VF453_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686884.1"
                     /translation="AEPPRRRLDRRWLWSGAAAVIALVFAGGLATATSKPRDQRTVSL
                     RDAHVLLQRELDAKAGGHLQVAELPDELVVEGLLDDAAQAKAAERAMDSVPPQYTVAR
                     KFSVATDVVETIRSALDMPGARISYRGGGVFAVDVDTNDVEATRAAIARVANDLAPTV
                     RRIDAVLGESPAPRPPMPAVLAAWVSGDGTNVMETRDGVKHLVPADEPASWPDRAAAE
                     PPIAMARQRRAP"
BASE COUNT          417 a         1178 c         1314 g          521 t
ORIGIN      
        1 gaggcggtcg aggccgggcc atcgcaggtc gtacacctcc gcgagcgtcg ccgaccagcg
       61 tgcgtccgcg gccgcggccg cgcggtcggc aaggccgaga cggcgcaggc cgcgctccat
      121 ccacgccggg tggagctcgg ccgcgacatc ggccgacttc cggcgcgcgg ccgccagcag
      181 gcgcgccagg agcgccgtgg gatcggcatc ctccgcggtc atggccgccc cgtggccgcg
      241 accggcgcgg cggcggcggc agcggcattg cgacgcttcc agaacatcca cccgccgccc
      301 gcggccgcga ggaccgccgc cagcgcgatc gcgaggacgg gcgatccgtc gccctccccg
      361 gccggcacgg gaggcgccgc cgggacgatc gccgccacca gcgtgacgtt gacgttctcg
      421 tacgtcagcc cttcgacgct gcgcaccacc aggttcttga ccgtgggcac gatggtggcg
      481 acgttgatgt cgggccggta cttgatgaag accgacgcgc tcgagggctt gacgtggtcg
      541 gccatcgggt cgttgttggg aagcacgacg tgcacgcgcg cggccaccac gccgtcgatg
      601 ctggccagcg tgcgttcgag ctcctgcgac acgccgtaga tgaagcgcac gcgctcctcg
      661 gtcggcgtgg agatgaggcc gtccttcttg aacatgtcgc ccaggctggc gcgatgctcg
      721 gcgggcaggc cgttcgcgtg cagcgtggcc agcgccgttc cgagctgcga cgactcgacc
      781 gtcacgtccc aggtcttgcc gtccgcggtc gtcttggccg catcgaggtg cgcgccgagc
      841 aggacctcca gcatgtcgtt ggcgtcgccc tcgctgagct tggtgaagac ctcctgcttg
      901 caggcggcca gcatgcaggc gaggccgagg gccagcgcga ggcgcgcgag gcgggatgcg
      961 atggaacctg ccatggccgc ctcactcgtt cttcagcagc gactgcaggc tcttggtgga
     1021 cgacttggtc acgcccatcg tcagcgacat cttgatgtgc gcctccgaga ccttgtccat
     1081 cagcatcatc gagccggcgg tgcgctcggt tgcgtccatc gacggcatgt gctcgatgaa
     1141 ggacgtcatg tcggactgca gctcgttgag cccggactgc tcgcggtcga tcatgccgcc
     1201 gatcgcggtc gcgttcttgc cggtggaatc ggccgcggct tcctgcgcgt ggcgctgcat
     1261 gagcgactcg aagcgctggc gcagctcggg cgcggcggcc gcctgcggcg tgccggcggc
     1321 gccggcggag ccgatcgagg cgagcgggtc gatcggcggg atgggggcga cgggcgtcat
     1381 gcgcgggctc ctggtcgggt gacggggccg ggcctcagca ggccaggccg acgaaggcgt
     1441 agtgatgggg cggcgacggc tcggcccggg cctcgaggcc gcgcagcgcg cgccccgacg
     1501 tgcgcgacag catctcgacg atgcccagcg ccaccgcgtc gcgcggcaac gccagcgctt
     1561 cctcggcgta ggcctcccag aggctgtcct tctggacgaa catgcacatc gccagcagcg
     1621 ccttgatcac ggcgttgcgc ggatgcttcg cctccgcctc ggtgagcagg tcgcgcgctt
     1681 ccgggtactg ccgggcgatc aggaggttgc gcgcctgcgc cgcgacgatc tcggggcggt
     1741 cggggaccga tgccgccatc acggcgagga tgcggccggt gtcggcctgc acgccgaact
     1801 tgtccgacag gacgtgcgcg gccacgatga tcctcatctg cgcgacgggc agggcgttgt
     1861 tctgcatggt gcggttcctt ggcgacgcgc gcggacgcga cgtcgacgaa tgaggaggcc
     1921 ggccgggacc ggcgtcggcc aggccggggc ggcgcgacgg cgcgccgtcc ccggaaggca
     1981 tcgcgaccgg gcgtcaggcc aggtccttca tgccgtcggc gccggtgttg gccagcttgt
     2041 tgcgcgcgtt gtccatcgcg acggcctggt ggttggccgc ggcgttgtcc tgctgcgcca
     2101 tgtcgatggc ggcggtctgg ttctgcatgt tcgcggcggc ggaggccatc gcggcctggt
     2161 tggccagcgc ggtgttctgc tgcgacaggt agttgctgac ggcgtcgccg gtcgagtcgt
     2221 tgatgttggt gacggcggcg ccgccgacgg cgctgccgac cccgctttgc accgcggtgt
     2281 tggcgccggt cttgcccatc tgcatgtact ggccggcggt ggtggcgccg tgggagactt
     2341 tgttggcatt cggcatgggg actcctgaaa cgtgtgatga acagccctca agggcgccgg
     2401 cgtggccggc gcatcgggag cgggctgggt ggctccgtcg aatcggggat caggccgcga
     2461 gctcgcggcc gtgcaacttg ttccacgccg cgcagacgcc gcgctgcgtg gccgcgaagg
     2521 cctcggccag caggccggcc atccgcttct cgtcgaagtc gatgctgcgc acctggctct
     2581 ggcgcgcctc gagctcgacc tgcgcgaagt agcggttcag gcgccgcgcg cgatcgccgc
     2641 tcgtgtcggt cttcagcgtg gcctcgagcg agtcgaaggc ggccagcagg tcgtcgggcg
     2701 gcagggtgaa ggcggtggtc atcggcatgt ctccgtcagg gggcccggcg ctggcgcgcc
     2761 atggcgatcg gtggttcggc cgcggcgcgg tcgggccagg aggcgggctc gtccgccgga
     2821 acgaggtgct tgacgccgtc gcgcgtctcc atcacgttcg tgccgtcgcc gctcacccag
     2881 gccgcgagca cggcaggcat cggcggccgg ggcgcgggcg actcgcccag caccgcgtcg
     2941 atgcggcgga cggtcggggc gaggtcgttc gccacgcggg cgatggcggc gcgggtcgcc
     3001 tcgacgtcgt tcgtgtccac gtcgacggcg aagacgccgc cgccgcggta ggagatccgc
     3061 gccccgggca tgtcgagggc ggagcggatc gtctcgacga cgtcggtggc cacggagaac
     3121 ttgcgcgcga ccgtgtactg cgggggcacc gagtccatcg cccgctcggc cgccttggcc
     3181 tgggcggcgt cgtccaggag gccctcgacc accagctcgt cgggcagctc cgcgacctgc
     3241 aggtggccgc cggccttggc gtcgagctcc cgctgcagca ggacgtgggc gtcgcgcagg
     3301 ctcaccgtgc gctggtcgcg cggcttggaa gtggcggtcg ccaggccgcc cgcgaacacg
     3361 agcgcgatca cggccgccgc ccccgaccac agccagcggc gatccaggcg ccgccgcggc
     3421 ggctcggccg
//
LOCUS       DASVLN010000183         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_9598, whole genome shotgun sequence.
ACCESSION   DASVLN010000183 DASVLN010000000
VERSION     DASVLN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9598"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..522)
                     /locus_tag="VF453_04210"
     CDS             complement(100..522)
                     /locus_tag="VF453_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7686885.1"
                     /translation="MLRQSSTNLAVETRGRGLVEITRPVAAWVAGTGMREGLLTLFVR
                     HTSASLLVQENADPDVRGDLDRFFARLVPDGDALFRHREEGPDDMPGHVRSALTAVQL
                     GIPLADGRLVLGTWQGIYLWEHRLRGHRREVAAHLVGE"
     gene            complement(647..>2261)
                     /locus_tag="VF453_04215"
     CDS             complement(647..>2261)
                     /locus_tag="VF453_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686886.1"
                     /translation="QGGDATVDDTLAYIAADISADPFDIAKGAQATIAAEVRMGDVVS
                     TVSGALTVLGDRPLPDGDSERLVHVTLTIATAHERIAYESGDLALHRTGDPAAGEYAI
                     DDAAHDPATPPLEFAWRGSPMAAIGVLLQAAKIVTIDGSRPSPAAGGDPTGGTGTGTG
                     TGAGTGNTTAERLVRLDALPAMPGLTDAAGAAAMDALIRIGDATGDDAFVARARRRLF
                     PTLDAPSRPVVSALRDWVMFRRRRTMPCCPGAAASVKSAIDTIKVWHLKVADAEACKA
                     LISVLDHANPAALAQFAFRPVGLLRYGDADTAPVESPDLVLAMWRAAQPGTQVVAGRY
                     WENAPATGQNWQNNFRLTAMLKQIAGLTTPPPTGQGAIAPLVDLAAPLLDTGYDGGML
                     IVTQDMGSVASNWRSLLADSRDFKPGAYHPLDAASPRTPLQYAGTAPVGNAVATYVST
                     VCSGTLRVRGLSLETTQAPDANAAARLAAVAAIVQGSGMTMPGAALGQVVAPMSPEDQ
                     RFLKAVGVDPASLDEVVYLELEDTGAASA"
BASE COUNT          270 a          814 c          873 g          304 t
ORIGIN      
        1 cggccggccg tggcgtttgg ctgagcatcg cggcgcgtca ggcaggctgg ggacggcgcg
       61 ctgcggcgcg ggctccccgt ggagcgggcg ggcggccgct cattcgccga cgaggtgcgc
      121 ggcgacctcc cggcgatgtc cgcgcaggcg gtgttcccac aggtagatgc cctgccatgt
      181 cccgagcacc agccgcccgt ccgccagcgg gatgcccagc tgcaccgccg tcaacgcgct
      241 gcggacgtgg cccggcatgt cgtcgggccc ctcctcgcgg tggcggaaca gcgcgtcgcc
      301 atcgggcacg aggcgggcaa agaagcggtc gaggtcgccg cgcacgtcgg ggtcggcgtt
      361 ctcctgcacc agcaggctgg ccgaggtgtg gcgcacgaac agcgtcagca gcccctcgcg
      421 catgcccgtg cccgcgaccc acgccgcgac cggccgcgtg atctcgacga gcccgcgccc
      481 gcgcgtctcg acggcgaggt tggtggacga ttgcctgagc atcgcccgag catagcgggc
      541 acgcgcgcgc tgcggtgtcg gacgagcgcc cggcgcacgg tgctcggctg gcgccggcgc
      601 ggatccggca tccgcgaccg gcggcgtcgc aggcaccgcg tcgccctcag gcgctggccg
      661 cgccggtgtc ctcgagctcg aggtacacca cctcgtcgag cgaggccggg tcgaccccga
      721 ccgccttcag gaagcgctgg tcctcgggcg acatcggcgc cacgacctgg cccagcgcgg
      781 cgccgggcat cgtcatgccg ctgccctgaa cgatcgccgc gaccgcggcc aggcgtgcgg
      841 cggcgttcgc gtccggcgcc tgcgtcgtct cgagcgacag gccgcgcacc cgcagcgtgc
      901 cgctgcacac cgtggacacg taggtcgcga cggcgttgcc gaccggcgcc gtgcccgcgt
      961 actgcaacgg cgtgcgcggg ctcgcggcgt cgagagggtg gtaggcgccg ggcttgaagt
     1021 cccgcgagtc ggccagcagc gagcgccagt tcgaggcgac gctgcccatg tcctgcgtga
     1081 cgatcagcat gcccccgtcg tagccggtgt ccagcagcgg ggccgcgagg tccaccagcg
     1141 gtgcgatcgc gccctggccg gtgggcggcg gcgtcgtgag gccggcgatc tgcttgagca
     1201 tcgccgtgag ccggaagttg ttctgccagt tctggccggt ggccggggcg ttctcccagt
     1261 agcgcccggc cacgacctgc gtgccgggct gcgccgcacg ccacatcgcg agcaccaggt
     1321 cgggcgactc gacgggcgcc gtgtcggcgt cgccgtagcg cagcagcccg accggccgga
     1381 acgcgaactg cgccagcgcc gcggggttcg cgtggtcgag caccgagatc agcgccttgc
     1441 aggcctcggc gtccgcgacc ttgaggtgcc agaccttgat cgtgtcgatc gccgacttca
     1501 cggaagcggc ggcgccgggg cagcatggca tcgtgcggcg gcggcggaac atgacccagt
     1561 cgcgcagcgc cgagacgacg ggtcgcgacg gcgcgtccag cgtggggaac aggcgtcgcc
     1621 gcgcacgtgc gacgaacgca tcgtcgccgg tcgcgtcgcc gatgcggatg agcgcgtcca
     1681 tcgccgccgc gccggcggcg tcggtgaggc cgggcatggc gggtagcgcg tccaggcgca
     1741 ccagccgctc ggccgtcgtg ttgcccgtgc cggcgccggt gcccgtacct gtacctgtac
     1801 cgcctgtcgg gtcgccgccc gcggccgggc tcggacggct gccgtcgatc gtgacgatct
     1861 tggcggcctg cagcagcacg ccgatcgccg ccatcggact gccgcgccac gcgaactcca
     1921 gcggcggcgt ggcggggtcg tgcgcggcgt cgtcgatggc gtactcgccc gcggcgggat
     1981 ccccggtgcg atgcagcgcg aggtcgccgg actcgtaggc gatgcgttcg tgcgcggtgg
     2041 cgatcgtgag ggtgacgtgg acgaggcgct cgctgtcgcc gtcggggagc gggcggtcgc
     2101 ccagcacggt cagcgcgccg ctgaccgtgg agacgacgtc gcccatgcgc acctcggcgg
     2161 cgatcgtcgc ctgcgcgcct ttggcgatat cgaacggatc ggcggagatg tcggctgcga
     2221 tgtaggcgag cgtgtcgtcg accgtcgcgt cgccgccttg c
//
LOCUS       DASVLN010000184         4273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_223530, whole genome shotgun sequence.
ACCESSION   DASVLN010000184 DASVLN010000000
VERSION     DASVLN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223530"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..822)
                     /locus_tag="VF453_04220"
     CDS             complement(13..822)
                     /locus_tag="VF453_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7686887.1"
                     /translation="MEHVATSSPTPRVVLQARGLVKRYGQVAALDGADFELRAGEILA
                     VIGDNGAGKSSLIKALAGAVRPDEGEILLDGAPANFRGPIDARHAGIETVYQDLAVAP
                     AMTIAENLFLGRELRRPGWRGRWLRMLDKRRMLEESVARLNELKVGIRSMTQPLETLS
                     GGQRQCVAVARAAAFARHVVIMDEPTAALGVKEGGMVLELIRRVRDRGLAVVLISHNM
                     PHVFEVADRVHVARLGRRCVVLDPKKIDMSDAVAVMTGALPPERLPREALA"
     gene            complement(824..1846)
                     /locus_tag="VF453_04225"
     CDS             complement(824..1846)
                     /locus_tag="VF453_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7686888.1"
                     /translation="MGAVHFHRPAAGGDPGSLAARLRARVGTVPSVATLGPFVALLAA
                     CAFFATQSDRFLAGGNLSLIAQQVAVVGVIAIGQTLVILTAGIDLSCGMVMALAGMVM
                     TKLASEGGVPPAVAILLGILVAAAFGLANGLLVTRVRLPSFIVTLGTMNIAFAATQLY
                     SNAQTITDLPPLFTSLGGTFTVGRTAVAWGTVAMLALYAIAWFLLRETAKGRHVYAVG
                     NNAEATRLVGIRTQRVLLGVYTLAGALYGIAAVLSVARTGVGDPNAGQTDNLDSITAV
                     VLGGTSLFGGRGGVLGTLVGALVVGVFRNGLTLIGVSSIYQVLVTGVLVILAVAADQL
                     SRKSSR"
     gene            complement(1846..2847)
                     /locus_tag="VF453_04230"
     CDS             complement(1846..2847)
                     /locus_tag="VF453_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7686889.1"
                     /translation="MRRRVPSLFAATLAIVAAVPAGAADQPVVGLITKTETNPFFVKM
                     KEGAEQAAKERGAKLLTAAGRKDGDNAGQVTAIENMVTAGAKTIMITPNDSKAIVPAI
                     RKARAQGVMVIALDSPADPRDATDALFATDNYKAGLLIGEYAKAALGGKPAHIVTLDL
                     FPGHPVGAQRHNGFMKGFGLAAPDAASNELGKASEIVCSADSYGDQAKGQTAMENCLQ
                     KSPEVNVVYTINEPAAAGAYTALQRAGKAKGVIIVSVDGGCAGVRNVKAGVIAATSQQ
                     YPLKMAALGVEAGVDYAKTGKKASGYTDTGVTLIADKPMPGIDSKDSATGLRLCWGQ"
     gene            3123..3860
                     /locus_tag="VF453_04235"
     CDS             3123..3860
                     /locus_tag="VF453_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7686890.1"
                     /translation="MDELTRHEILIVDDDDNLCDTLSAVLEAAGFAAQCAADGATMMS
                     KLARGAFSLVLIDLRLRNEDGMQLARELRQRSRIPIIMVTGQGDDTDRVLGLETAVDD
                     FVMKPFNNRELVARIRALLRRSTEVGMPRSTRADPVGHARYRFDRWIVNLSTRTLSHA
                     DGTRCELTQGEFALLEAMVLSPNRVISREQLLTMTRGADSATTDRTIDVLILRLRKKL
                     EVNPALPTIIKTERGLGYRFAVDVVRC"
     gene            complement(3879..>4273)
                     /locus_tag="VF453_04240"
     CDS             complement(3879..>4273)
                     /locus_tag="VF453_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7686891.1"
                     /translation="ASQVPPGLRILLVEDDDDVRPALEDVLARLEAEVLAARSLPEAV
                     EALQAHAVDLVLSDVGLGPQGDGLALRAWVIERCPGTPVVVMSGLPSDLLAQRYEGST
                     RVPILRKPFTADELAAAISAALEAASDA"
BASE COUNT          599 a         1551 c         1533 g          590 t
ORIGIN      
        1 gcccgctccc cgtcaggcca gcgcctcgcg cggcaggcgc tccggcggca gcgcgcccgt
       61 catgacggcc accgcgtcgc tcatgtcgat cttcttcgga tcgagcacga cgcagcgccg
      121 gcccagccgc gccacgtgga cgcggtcggc cacctcgaag acgtgcggca tgttgtgcga
      181 gatgagcacc acggcaagcc cgcggtcgcg cacgcgacgg atcagctcga gcaccatgcc
      241 gccctccttg acgccgagcg cggcggtcgg ctcgtccatg atcacgacgt ggcgcgcgaa
      301 cgccgcggcg cgcgccacgg cgacgcactg gcgctggccg ccggacagcg tctccagcgg
      361 ctgcgtcatc gagcggatgc cgaccttgag ctcgttcagc cgcgcgacgc tctcctcgag
      421 catgcggcgc ttgtccagca tgcgcagcca gcggccgcgc cagcccgggc ggcgcagctc
      481 gcggccgagg aacaggttct ctgcgatggt catcgcgggc gcgacggcga ggtcctggta
      541 gaccgtctcg atgccggcgt gccgggcgtc gatcgggccg cggaagttcg cgggcgcgcc
      601 gtccagcagg atctcgccct cgtcgggccg cacggcgccg gccagggcct tgatgagcga
      661 ggacttgccc gcgccgttgt cgccgatgac ggcgaggatc tcgccggcgc gcagctcgaa
      721 gtcggcgccg tccagggcgg cgacctgtcc gtagcgcttg acgagtccgc gcgcctgcag
      781 caccacgcgc ggcgtcgggg aggaagtggc gacgtgttcc atgtcagcga ctcgacttgc
      841 gggagagttg gtctgcggcg accgccagga tcaccagcac gccggtgacg agcacctggt
      901 agatcgacga cacgccgatc agcgtcagcc cgttgcggaa cacgccgacg accagcgcgc
      961 ccaccagcgt gcccagcacg ccgccgcggc cgccgaacag gctcgtgccc ccgagcacga
     1021 cggccgtgat cgagtcgagg ttgtcggtct ggccggcgtt cgggtcgccg acgcccgtgc
     1081 gcgcgacgga cagcaccgcc gcgatgccgt acagcgcacc cgcgagcgtg tagacgccca
     1141 gcagcacgcg ctgcgtgcgg atgcccacca ggcgcgtcgc ttccgcgttg ttgccgacgg
     1201 catagacgtg gcggcccttg gcggtctcgc gcagcaggaa ccaggcgatc gcgtacagcg
     1261 ccagcatcgc caccgtgccc cacgcgaccg ccgtgcggcc gacggtgaag gtgccgccca
     1321 gcgacgtgaa cagcggcggc aggtcggtga tggtctgcgc gttggagtac agctgggtcg
     1381 ccgcgaacgc gatgttcatc gtgcccagcg tcacgatgaa cgacggcagg cgcacgcgcg
     1441 tgacgagcag gccgttggcc agcccgaacg ccgccgccac gaggatgccc agcaggatcg
     1501 cgacggccgg cggcacgccg ccctccgacg cgagcttggt catcaccatg ccggccagcg
     1561 ccatgaccat cccgcacgac aggtcgatgc ccgcggtgag gatgaccagc gtctgcccga
     1621 tcgcgatcac gccgacgacg gcgacctgct gggcgatcag cgacaggttg ccgccggcca
     1681 ggaagcggtc cgactgcgtg gcgaagaacg cgcaggcggc cagcagcgcg acgaaggggc
     1741 ccagcgtcgc gacgctgggg acggtgccga cgcgcgcgcg caggcgggcg gccagcgagc
     1801 cggggtcgcc cccggcggcg gggcggtgga agtgcacggc gcccatcact ggccccagca
     1861 caggcgcagg ccggtcgcgg agtccttgct gtcgatgccg ggcatcggct tgtcggcgat
     1921 cagcgtgacc ccggtgtcgg tgtagccgct ggccttcttg cctgtcttcg cgtagtcgac
     1981 gcccgcctcg acgccgagcg cggccatctt cagcgggtac tgctgcgagg tcgcggcgat
     2041 gacgccggcc ttgacgttgc gcacccccgc gcagccgccg tccaccgaga cgatgatcac
     2101 gcccttggcc ttgccggcgc gctgcagcgc ggtgtaggcg ccggcggcgg cgggctcgtt
     2161 gatcgtgtag acgacgttga cctcggggct cttctgcagg cagttctcca tcgcggtctg
     2221 gcccttggcc tggtcgccgt agctgtcggc cgagcacacg atctcggagg ccttgccgag
     2281 ctcgttgctg gccgcgtcgg gcgccgcgag gccgaagccc ttcatgaagc cgttgtggcg
     2341 ctgcgcgccc accgggtggc cggggaagag gtccagcgtg acgatgtgcg ccggcttgcc
     2401 gcccagcgcc gccttcgcgt actcgccgat cagcaggccg gccttgtagt tgtcggtggc
     2461 gaacagcgcg tcggtcgcgt cgcgcgggtc ggccgggctg tccagggcga tcaccatcac
     2521 gccctgcgcg cgcgccttgc ggatcgccgg cacgatcgcc ttggagtcgt tgggcgtgat
     2581 catgatggtc ttcgcgcccg cggtgaccat gttctcgatc gccgtgacct ggcccgcgtt
     2641 gtcgccgtcc ttgcgacccg cggcggtcag cagcttcgcg ccgcgctcct tggccgcctg
     2701 ctccgcgccc tccttcatct tgacgaagaa cgggttggtc tcggtcttgg tgatgaggcc
     2761 caccaccggc tggtcggccg cgcccgcggg gacggcggcg acgatagcga gggtggcggc
     2821 gaacagggaa gggacgcggc ggcgcatggg ttgtctcctg ggaatgtcgg ttcggttcgg
     2881 atcgtccgga cgccgccgcc ggcgcctaca cttcgcaagc gcgcggccgg ggtccgtccg
     2941 cggcatttgg cggcgcggcg gttaacgagc ggtgaaggcc gccggcggcc cctgcaatag
     3001 atgaaacatt ccaggccgcc tccggcctcc gtttcaaatg tgaacgagcc cgacccgccg
     3061 cgacatcggc cgttaccgcc ggacggctcc aatgacgcgc gtacgggcag cgggaacgca
     3121 tcgtggatga actgaccagg cacgagatcc tgatcgtcga cgacgacgac aacctgtgcg
     3181 acacgctgtc ggcggtgctc gaggccgccg gcttcgccgc ccagtgcgcc gccgacggcg
     3241 cgacgatgat gagcaagctc gcgcgcggcg cgttctcgct cgtgctgatc gacctgcggc
     3301 tgcgcaacga ggacggcatg cagctcgcgc gcgagctgcg ccagcgctcg cgcatcccca
     3361 tcatcatggt caccggccag ggcgacgaca ccgaccgcgt gctgggcctg gagaccgcgg
     3421 tcgacgactt cgtcatgaag ccgttcaaca accgcgagct cgtcgcccgc atccgcgcgc
     3481 tcctgcgccg cagcaccgag gtcggcatgc cgcgctcgac gcgcgccgac ccggtcggcc
     3541 acgcccgcta ccgcttcgac cgctggatcg tcaacctgag cacgcgcacc ctgtcgcatg
     3601 ccgacggcac gcgctgcgag ctgacgcagg gcgagttcgc gctgctcgag gcgatggtgc
     3661 tgtcgcccaa ccgcgtgatc tcccgcgagc agctgctgac gatgacgcgc ggcgccgact
     3721 ccgccacgac ggaccgcacg atcgacgtcc tcatcctgcg cctgcgcaag aagctggagg
     3781 tcaacccggc gttgccgacg atcatcaaga ccgagcgggg gctgggctac cggttcgcgg
     3841 tcgacgtggt gcgctgctga acgacgcccg cggcggcgtc aggcatcgga cgcggcctcg
     3901 agcgccgcgg agatcgcggc cgcgagctcg tcggccgtga agggcttgcg caggatcggc
     3961 acgcgcgtgc tgccctcgta gcgctgcgcc agcaggtcgc tcggcaggcc cgacatcacg
     4021 acgaccggcg tgccggggca gcgctcgatc acccaggccc gcagcgcgag cccgtcgccc
     4081 tggggcccga ggcccacgtc cgacagcacg aggtcgaccg catgcgcctg cagcgcctcg
     4141 accgcctcgg gcagcgagcg cgcggccagc acctcggcct ccagccgcgc gaggacgtcc
     4201 tccagcgccg gccgcacgtc gtcgtcgtcc tccacgagca ggatgcgcag cccgggcggc
     4261 acctgcgagg cgg
//
LOCUS       DASVLN010000185         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_439492, whole genome shotgun sequence.
ACCESSION   DASVLN010000185 DASVLN010000000
VERSION     DASVLN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439492"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VF453_04245"
     CDS             complement(<1..600)
                     /locus_tag="VF453_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX7686892.1"
                     /translation="MTTDPVSDERARAWRGVRRLLAVRVDNLGDVLMTTPALAAAAAS
                     APGIEITLLGGSGASALAPHLPMVHDVMTARVPWVRHEDGAATVDDAALVARLAERCF
                     DAAVVFTVCTQSALPAAVLCRLAGIPLRLAHVRENPYQLLSDWAPELDLSVSGARHEV
                     RRQLDLVARVGWRCADERLRFALRGPDVARVDAMLGRDDG"
     gene            complement(597..>1796)
                     /locus_tag="VF453_04250"
     CDS             complement(597..>1796)
                     /locus_tag="VF453_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX7686893.1"
                     /translation="MVPRKGVETVVRAMACAPCAGMRLRVVGGDSAPADPLRTPEIGR
                     LQAIARECGVEDRVVFEGRQPRSVLRDWYAASDVFVTTPWYEPFGITPLEAMACARPV
                     VGSAVGGVRSTVVDGETGLLVPPRNPEALAHALATLRAHPSRATAMGEAGLARARAEY
                     TWTGVAQRLAEVFAEVTAAPAPSRSVFHVAGLDIPVPVPAAARPVPAIFLDKDGTLVE
                     DVPFNVDPARLRFTPHAIEALRLWRDAGWRLVVITNQPGLGRGLFDRAALARLHEGLA
                     RRLADAGIVLDGFYACPHVEGEGCDCRKPRDGLLRTAARELGIDLGRSWMVGDILNDV
                     EAGHRAGCRSVLLDTGGETEWVPGPLRMPELRCADLLEAAVATLSPDEIPAVVPPRTA
                     SSAGAVA"
BASE COUNT          243 a          683 c          677 g          193 t
ORIGIN      
        1 cccgtcgtcg cgaccgagca tcgcgtcgac gcgcgcgaca tccgggccgc gcagggcgaa
       61 gcgcagccgc tcgtcggcgc agcgccagcc gacgcgggcg acgaggtcga gctggcgccg
      121 aacctcgtgg cgcgcgccgg agacggacag gtcgagctcc ggcgcccagt cgctgagcag
      181 ctggtacggg ttctcgcgca cgtgcgccag gcgcagcggg atgccggcga gcctgcacag
      241 cacggccgcg ggcagcgcgc tctgggtgca gaccgtgaag acgacggccg cgtcgaagca
      301 gcgctcggcc aggcgcgcga ccagcgccgc gtcgtcgacg gtggcggcgc cgtcctcgtg
      361 ccgcacccag ggcacgcgcg cggtcatcac gtcgtgcacc atgggcaggt gcggcgccag
      421 ggcggacgcg ccggagccgc ccagcagcgt gatctcgatg cccggcgccg aggctgcggc
      481 ggcggcgagc gccggcgtcg tcatcagcac gtcgccgagg ttgtcgacgc gcacagccag
      541 cagccggcgc acgccgcgcc aggcgcgtgc gcgttcgtcc gacacgggat ccgtcgtcac
      601 gcgaccgccc ccgccgacga tgcggttcgc ggcggcacga cggcggggat ctcgtcgggc
      661 gagagcgtcg cgaccgcggc ctccagcagg tcggcgcagc gcagctcggg catgcgcagc
      721 gggcccggca cccactcggt ctcgccgccg gtgtccagca gcacgctgcg gcaacccgcg
      781 cgatggccgg cctcgacgtc gttgaggatg tcaccgacca tccacgagcg cccgagatcg
      841 atgccgagct cgcgcgcggc cgtgcgcagc aggccgtcgc gcggcttgcg gcagtcgcag
      901 ccctcgccct ccacgtgcgg gcaggcgtag aagccgtcca gcacgatgcc cgcgtcggcc
      961 agccgcctcg cgaggccctc gtgcaggcgg gcaagcgccg cgcggtcgaa caggccgcgt
     1021 ccgaggcccg gctggttcgt gatcacgacc aggcgccagc cggcgtcgcg ccacaggcgc
     1081 agcgcctcga tcgcatgcgg cgtgaagcgc aggcgcgcgg ggtcgacgtt gaaaggcacg
     1141 tcctcgacca gcgtgccgtc cttgtcgagg aagatcgccg gcaccggtcg cgccgcggcg
     1201 ggtaccggaa cgggtatgtc gaggcccgcg acatggaaga cggagcgcga cggcgccggc
     1261 gcggcggtga cttccgcgaa gacctcggcc aggcgttgcg ccacgcccgt ccaggtgtac
     1321 tcggcccgcg cgcgcgcgag cccggcctcg cccatcgcgg tggcgcgcga cggatgcgcg
     1381 cgcaaggtcg cgagcgcgtg cgccaatgcc tcgggattcc tgggcggcac cagcaggccc
     1441 gtctcgccgt cgacgaccgt cgagcgcacg ccgcccaccg cgctgccgac gacgggccgc
     1501 gcgcaggcca tcgcctccag cggcgtgatg ccgaacggct cgtaccaggg cgtcgtcacg
     1561 aagacgtcgc tggcggcgta ccagtcgcgc aggaccgaac gcggctggcg accttcgaag
     1621 accactcggt cctcgacccc gcattcgcgc gcgatggcct gcaggcggcc gatctcgggc
     1681 gtgcgcaggg ggtcggcggg cgcgctgtcg ccgcccacga cgcgcagccg catgcccgcg
     1741 cacggcgcgc aggccatcgc gcgcacgacc gtctccacgc ccttgcgcgg caccat
//
LOCUS       DASVLN010000186         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_278181, whole genome shotgun sequence.
ACCESSION   DASVLN010000186 DASVLN010000000
VERSION     DASVLN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278181"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VF453_04255"
     CDS             complement(<1..333)
                     /locus_tag="VF453_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686894.1"
                     /translation="MRYRTLLLYLDDQPGAGACADHAIRLAREFEGHLLGLSCHRPVP
                     WPDEDADGRGDVLIPELRAAEDAAREREAAFAQRCAKAGLGSCGTLRDDAEPGAAVAR
                     RALAADLVV"
     gene            complement(417..1229)
                     /locus_tag="VF453_04260"
     CDS             complement(417..1229)
                     /locus_tag="VF453_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX7686895.1"
                     /translation="MMIARALRRVAILLVAWIAGAAAQVPVPPAAAHVIDQAGALESG
                     QAAALEARLAGFEREHGAQVVVLIVPTTAPETIEQYGIRVADQWKLGRRGVDDGVILL
                     LAMQDRAARIEVGYGLEGTLPDATAHRILAEDMVPRLQAGDIAGAVTAGVDRILAVVA
                     GTPLPPPRDDEDVRSTTTLVTVAMASVAVGGLLRRLIGRPRAAALTGGGAGLLAAWLA
                     GSWAVGLLAGAIAAALAFTGVFGWLPWSSGGGGSSFRGRGGGFGGGGASGRW"
     gene            complement(1226..1882)
                     /locus_tag="VF453_04265"
     CDS             complement(1226..1882)
                     /locus_tag="VF453_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7686896.1"
                     /translation="MIARALIGFPLRHLLAFVLALLVGNLAGCGYNRLVSADENVKAA
                     WSEVLNQYQRRNDLVPALVEVVKADAAFERSTLMQVVEARARATAVQATPALVDDPEA
                     FARFERAQAQVGGALSRLLVVAENYPTLQANQAFRDLQAQLEGTENRIAVARHRYIEA
                     VNTYNTEVRSLPSNMTAMAFGFHAKANFRTDDDAAVARPPHIDMAGAFAPSAPASARP
                     "
     gene            complement(1985..>2434)
                     /locus_tag="VF453_04270"
     CDS             complement(1985..>2434)
                     /locus_tag="VF453_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7686897.1"
                     /translation="EEVILHSPRPSLLLPYAGRFEAIGRTALVAWDGSHAAARAASDA
                     LPLLKRASAVHLVAFDREVGEARTLDHAPLLALARWFGRHAVDVQPRACFPGTDIGNA
                     LLSHAADVGADLLVMGCWGHARLAERLLGGATRTVLESMTLPVLTSR"
BASE COUNT          335 a          903 c          912 g          284 t
ORIGIN      
        1 cacgacgagg tcggcggcca gcgcccgcct ggcgacggcg gcccccggtt cggcgtcgtc
       61 gcgcaaggtc ccgcacgacc cgagcccggc cttcgcgcaa cgctgcgcga acgcggcctc
      121 gcgttcccgc gccgcatcct cggccgcccg cagctccggg atgagcacat cgccgcggcc
      181 gtcggcgtcc tcgtcgggcc agggcacggg ccggtggcac gagagtccca gcaggtggcc
      241 ctcgaactcg cgcgcgagcc ggatcgcatg atcggcgcag gcgccggccc ccggctggtc
      301 gtccaggtag agcaacaggg tgcggtagcg catgcggatc tccggcgatc gcgacgcggc
      361 gattgtgcgg aactcgacgc cgccggcgct tgcgccgacg caacgaccgg acgcggctac
      421 cagcgcccgg acgccccgcc tccgccgaag ccgccgccac gtccgcggaa ggacgatccg
      481 ccgccgccgg acgaccacgg cagccagccg aagacgccgg tgaacgcgag cgccgccgcg
      541 atcgcgccgg ccagcaggcc gacagcccag gacccggcaa gccaggcggc cagcaggccc
      601 gcgccaccgc cggtgagcgc ggctgcgcgc gggcggccga tcagtcggcg cagcagtccg
      661 ccgacggcca ccgaggccat cgccacggtg acgagcgtcg tcgtcgagcg cacgtcctcg
      721 tcgtccctcg gcggcggcaa gggcgtgccg gccacgaccg cgaggatgcg gtcgaccccg
      781 gccgtcaccg cgccggcgat gtcgcccgcc tgcaggcgcg gcaccatgtc ctccgccagg
      841 atgcggtgcg ccgtcgcatc gggcagggtg ccctcgagac cgtagccgac ctcgatgcgc
      901 gccgcgcgat cctgcatcgc gagcagcagg atcacgccgt cgtcgacgcc gcggcggccg
      961 agcttccact ggtcggcgac gcggatgccg tactgctcga tcgtctccgg cgcggtggtc
     1021 ggcacgatca ggacgaccac ctgcgcgccg tgctcgcgct cgaagccggc gagccgcgcc
     1081 tccagcgccg cggcctggcc ggattccagc gcgccggcct ggtcgatcac gtgcgcagcc
     1141 gcgggcggta ccgggacctg cgcggcggcg ccggcgatcc aggcgaccag caggatcgcg
     1201 acccggcgca gcgcgcgggc gatcatcatg gccgggcgct cgccggcgcc gagggagcga
     1261 acgcgcccgc catgtcgatg tgcggcggac gcgccaccgc ggcgtcgtcg tcggtgcgga
     1321 agttcgcctt ggcgtggaag ccgaacgcca tcgcggtcat gttggacggc aggctgcgca
     1381 cctcggtgtt gtaggtgttg acggcctcga tgtagcgatg gcgcgcgacg gcgatgcggt
     1441 tctccgtccc ttccagctgc gcctgcaggt cgcggaacgc ctggttcgcc tgcagcgtcg
     1501 gatagttctc ggcgaccacc agcaggcgcg acagcgcgcc gccgacctgc gcctgggcgc
     1561 gctcgaagcg ggcgaaggcc tcgggatcgt cgaccagcgc gggggtcgcc tgcacggccg
     1621 tcgcgcgcgc gcgcgcctcg acgacctgca tcagcgtcga gcgctcgaac gccgcgtcgg
     1681 ccttgacgac ctcgaccagc gccggcacga ggtcgttgcg tcgctggtac tggttcagca
     1741 cctccgacca ggcggccttg acgttctcgt cggcgctcac gaggcggttg tagccgcagc
     1801 ccgcgaggtt gccgaccagc agcgcgagca cgaacgccag caggtggcgc aggggaaatc
     1861 cgatcaaggc gcgagcgatc atggggtcat cctaggaagg tcggccgcgc aggccttgag
     1921 gccgatcaat gcgtcttgcg cggccgagcg gggccgaggc gcatcgaggt ccgccggcgc
     1981 ccggtcagcg cgaggtcagc acgggcagcg tcatgctctc gagcacggtg cgggtcgcgc
     2041 cgccgagcag gcgctcggca aggcgcgcat ggccccagca gcccatgacg agcaggtcgg
     2101 cgccgacgtc ggccgcgtgc gacagcaatg cgttgccgat gtcggtgccc gggaagcatg
     2161 cgcggggctg gacgtcgacc gcgtggcgac cgaaccagcg ggcgagggcc agcagcggcg
     2221 catggtcgag ggtccgcgcc tcgccgactt cgcgatcgaa ggcgacgaga tgcaccgcgc
     2281 tcgcccgctt gagcagcggc agcgcgtcgg aggccgcgcg cgcggcggca tggctgccgt
     2341 cccaggcgac gagcgccgtc ctgccgatcg cctcgaagcg cccggcatag ggcagcagca
     2401 ggctcgggcg cgggctgtgc aggatcacct cctc
//
LOCUS       DASVLN010000187         6879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_170944, whole genome shotgun sequence.
ACCESSION   DASVLN010000187 DASVLN010000000
VERSION     DASVLN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170944"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF453_04275"
     CDS             <1..216
                     /locus_tag="VF453_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7686898.1"
                     /translation="RTPLLARIVAPTLVIHGEADPLVPVANGRDLAARIAGAQADFIA
                     GMGHDLPDELLERFADRIHANARRADA"
     gene            complement(532..2931)
                     /locus_tag="VF453_04280"
     CDS             complement(532..2931)
                     /locus_tag="VF453_04280"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEX7686899.1"
                     /translation="MKTSGHKMKLLPALVLGALAISAHAAVSTAWVATATKAHDPRNA
                     VHVAPMRAGDQVPVVISLKLRNKAQLDAFTAKLMAGDKSVHPLTSAEFMAQYAPSNEQ
                     VARVVSYLQTHGFTGIEVAPNHLLVSATGGAGVIKDAFKAELHTYDVNGRSAYANVTD
                     ALVPASLGDTVLAVIGLQTVHLAHTNIKHAEAVKTQAATPQAVTGVSIPNFASIYGAS
                     SLPSATNATIGIITAGSLTQTIKDLKSFASSAGYPAPPVTTTVVGTAGTSTSGTDEWN
                     MDSQSSLAAAGGTIKSMILYNVNSLSDSALSQGYNRAVTDNKARAINVSLGGCESDEG
                     SVEASQDQIFQTAVSQGQMFSVSSGDSGAYECGATAGKAQSYPAVSPYVIAVGGTTLS
                     TNGATWAGETVWSCTSASSCQQSSSGGAGGGPSLTENAPSWQTAAGVLGSSTKRGVPD
                     ISFDAAPSSGALVLVNGQQAQIGGTSLAAPLWAGFWSRIQSANNNTLPFPAQTLYQGA
                     ASHPNWFHDVTSGNQGYAAATGWDYASGYGSVQIANFATAFSGGGCTSNCSTPVANFT
                     DTVSNLTATFTDSSTDTGGTISSYSWNFGDGSTSTTKSPVHTYAAAGTYTVTETVTDN
                     LGATSTKSASVTVSSSGGGGSQVVTNGGFESGSTGWTASSGVICTNSGCSGETAHSGT
                     GFAWMDGYGSSHTDSVSQSVTIPSGKSSASLSFYLHIDTAETTKTSAYDKLTVSVTSG
                     STTTTLGTFSNLNAASGYVLKSFSMTPYIGKTVTIKFTGTEDSSLQTSFVLDDVSLTA
                     Q"
     gene            complement(3607..5916)
                     /locus_tag="VF453_04285"
     CDS             complement(3607..5916)
                     /locus_tag="VF453_04285"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX7686900.1"
                     /translation="MEKNTASLSPAEQALRDAALEYHRSPVRGKIAVTPTKPLSNQRD
                     LSLAYSPGVAYACLAIEEDPTLAHDYTARGNLVGVVTNGTAVLGLGDIGPLAGKPVME
                     GKGCLFQKFAGIDVFDIELAERDPDKLVDIIAALEPTLGGVNLEDIKAPECFYIEKKL
                     RERMNIPVFHDDQHGTAIISSAALLNGLELVGKRIGEVKIAVSGAGAAAIACLDLMCA
                     LGVRRENVFVCDSKGVIHDRREDKLDESKKRYQQVTAARTLADVMKGADVVLGCSAAG
                     AVTADMVASMADRPIILALANPEPEIRPELAKSVRPDCIVATGRSDYPNQVNNVLCFP
                     YIFRGALDCGATRITEEMKVACVREIAELAKAEVSGEVAAAYAGRELRFGPDYIIPTP
                     FDSRLILRIAPAVAKAAEASGVATRPIADHDAYVQSLDRFVSHTGLVMRPLFGAARQA
                     PKRVAYAEGEDERVLRAVQIALDERLVKPILIGRPAVIEARIERAGLRIKAGRDFEVV
                     NPEDDTRFRQYWEAYHRLNARDGVTAEMAKATVRRSNTTIAALMVHLGDADALLCGLV
                     GRFDGHFSHLREVIGLRPGAHGFATMNALLLDKHTLFIADTSVNDDPSAEQLADIAAM
                     AVKEVRRFALEPRVAFLSHSMFGSSQRPSARKMRAARDLFVARMPEVPADGEIQGDAA
                     FSPELRAAILPDSTLTGSANVLVCPNLDAANILFNVLKMTGGHGVTIGPMLLGANAPV
                     HVLTPSSTVRRIVNMTALVVAHAATSGAA"
     gene            complement(6107..6565)
                     /locus_tag="VF453_04290"
     CDS             complement(6107..6565)
                     /locus_tag="VF453_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7686901.1"
                     /translation="MYRAIVRRKLRRAFEDINAGRYDGIVGQFAREHRHAMAGRHPLA
                     GERRTVASTARWYERLQRLLPGLAFEIRAIVVGGTPWRTHAAVAWDDRFTLPDGTVGC
                     NSGVHEFELAWGRVRSLVIHCDTERLHDYCRRIAALGVAEASAAPITDVR"
     gene            6647..>6879
                     /locus_tag="VF453_04295"
     CDS             6647..>6879
                     /locus_tag="VF453_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7686902.1"
                     /translation="MPASRPSAKVAKPAKPAKPAKGAPAPRPYHHGDLRESLLEVAAR
                     WLDERGAATLTLRELAKAAGVSHAAPYHHFAGL"
BASE COUNT          966 a         2293 c         2519 g         1101 t
ORIGIN      
        1 cgcacgccgc tgctcgcgcg catcgtcgcg ccgacgctcg tgatccatgg cgaagccgat
       61 ccgctggtgc cggtcgccaa tggccgcgac ctcgccgcgc gcatcgcggg cgcgcaggcc
      121 gacttcatcg ccggcatggg ccacgacctg cccgacgaac tgctggagcg cttcgccgac
      181 cgcatccacg ccaatgcgcg ccgcgcggac gcgtgaagcg gcgcatcccg gtgccggctt
      241 cgtgacgata cggtgacccg tatgcccgcc cgcactgcga gcacggacat cggagagaat
      301 gcgcgctgac cgccgcgggc cgcgaaggcg ttgcgtcacg ccaactcctg cgccggggac
      361 agcgcgaaag acccgccgcg cagcggggcg acttgtcccg gccgcgccgg gggtgcccat
      421 gcagcacggc gcgcccggca gggaaaaagg gccgcgcggt tgcccgggcg gccctggtcg
      481 cgatcgtcgg caggccggcg cagcggcgcc gaccggcctc gagcgcgcgg cttactgcgc
      541 ggtcagcgag acgtcgtcca ggacgaacga ggtctggagc gacgagtcct ccgtgccggt
      601 gaacttgatc gtgaccgtct tgccgatgta gggcgtcatg ctgaaggact tcagcacgta
      661 gcccgaggcg gcgttcaggt tcgagaaggt gcccagcgtc gtggtcgtgc tgccgctggt
      721 gaccgagact gtcagcttgt cgtacgccga ggtcttggtc gtctcggccg tgtcgatgtg
      781 caggtagaac gacagcgagg cgctcgactt gcccgacggg atcgtcaccg attgcgagac
      841 gctgtcggtg tggctcgagc cgtagccgtc catccaggca aagccggtgc ccgagtgggc
      901 cgtctcgccg ctgcagccgc tgttcgtgca gatcacgccc gagctggccg tccagccggt
      961 ggagcccgac tcgaagccgc cgttggtgac gacctgcgag ccgccgccgc ccgaggacga
     1021 cacggtcacc gaggcggact tcgtcgacgt cgcgcccagg ttgtccgtca cggtctcggt
     1081 cacggtgtac gtgccggccg cggcgtaggt gtggacgggg ctcttggtcg tcgaggtcga
     1141 gccgtcgccg aagttccacg agtacgagct gatcgtgccg cccgtgtcgg tggaggaatc
     1201 ggtgaacgtg gccgtcaggt tcgagaccgt gtcggtgaag ttcgcgaccg gcgtcgagca
     1261 gttgctcgtg cagccgccgc ccgagaaggc ggtggcgaag ttggcgatct gcacggagcc
     1321 gtagcccgag gcgtagtccc agccggtggc cgcggcgtag ccctggttgc ccgaggtcac
     1381 gtcatggaac cagttcggat gcgacgccgc gccctggtag agcgtctgcg ccgggaacgg
     1441 cagcgtgttg ttgttggccg actggatgcg cgaccagaag ccggcccaca gcggcgcggc
     1501 cagcgaggtg ccgccgatct gggcctgctg gccgttgacc agcaccagcg cgccgctgct
     1561 gggcgcggcg tcgaacgaga tgtcgggcac gccgcgcttg gtggacgagc cgagcacgcc
     1621 ggcggccgtc tgccagctgg gcgcgttctc cgtcagcgag gggccgccgc cggcgccgcc
     1681 ggacgacgac tgctggcagc tcgaggcgct ggtgcacgac cacaccgtct cgccggccca
     1741 cgtggcgccg ttggtcgaca gcgtcgtgcc gcccaccgcg atgacgtagg gcgagaccgc
     1801 cgggtagctc tgcgccttgc ccgcggtggc gccgcactcg tacgcgccgg agtcgcccga
     1861 cgagaccgag aacatctggc cttgcgacac ggcggtctgg aagatctggt cctgcgacgc
     1921 ctcgaccgag ccttcgtcgc tttcacagcc gcccagcgag acgttgatcg cgcgcgcctt
     1981 gttgtccgtc acggcgcggt tgtagccctg cgacagcgcc gagtcgctca gcgaattgac
     2041 gttgtacagg atcatggact tgatcgtgcc gccggcggcc gccagcgagc tctgcgagtc
     2101 catgttccat tcgtcggtgc ccgacgtgga ggtgccggcg gtgccgacga cggtcgtcgt
     2161 gaccggcggc gcggggtagc cggcgctgga ggcgaagctc ttcaggtcct tgatggtctg
     2221 ggtcagcgag cccgcggtga tgatgccgat ggtcgcgttc gtggcgctcg gcaggctcga
     2281 cgcgccgtag atggacgcga agttcgggat gctgacgccc gtcaccgcct gcggggtcgc
     2341 cgcttgcgtc ttgacggcct ccgcgtgctt gatgttcgtg tgggccaggt ggacggtctg
     2401 caggccgatg acggcgagca cggtgtcgcc gagcgacgcg ggcacgagcg cgtcggtgac
     2461 gttggcgtac gcggagcggc cgttcacgtc gtacgtgtgc agctccgcct tgaaggcgtc
     2521 cttgatgacg ccggcgccgc cggtggcgct caccagcagg tggttcgggg cgacctcgat
     2581 gccggtgaag ccgtgcgtct gcaggtagct cacgacgcgc gcgacctgct cgttgctcgg
     2641 cgcgtactgg gccatgaact cggcgctggt gagcgggtgc acgctcttgt cgccggccat
     2701 cagcttggcc gtgaaggcgt cgagctgggc cttgttgcgc agcttcagcg agatgacgac
     2761 cggcacctgg tcgcccgcgc gcatcggcgc cacgtgcacg gcattgcgcg ggtcgtgcgc
     2821 cttggtcgcg gtggcgaccc aggccgtcga cacggcggcg tgggcggaga tcgcgagcgc
     2881 gcccagcacg agggcgggca gcagcttcat cttgtgtcct gaggtcttca tggaactctt
     2941 ctcctgatcg atgggtcagt cgcgcaaatg tcggcgcgag ttttttgcta tgggagcggc
     3001 aattcgattt tgtcgacgtc gcccggtgga gcatcggcgg tcaccggttg gtggaattcc
     3061 aattcggggg gaggccgccg atgcaggcgc cgattctgtt cccgcgcaat ggaggcggaa
     3121 tcaggtcgaa ccccgagggg tatcgaggtg cactcaatgc acctcgaccg tgccaattag
     3181 ttgaagttgg tgcgcactcc aattcgctca tcttctgctt gaaagagttg tatccggttg
     3241 caccattgct cctccgggtg cgccattaat gcgtcattaa ttgccgcgcc ggcaatgatt
     3301 caggattaaa tccggacgcc gggattacgg gaattcccat ttcgggaaat ccctctccgg
     3361 gatttggtgg aaagaccatt cccgaattgc acatggttat taccgatgca atgcgcggcg
     3421 catggtcgtt cgtgccggcg gtgcgcgacg cggccgcgac gccaaatccg gacagtctga
     3481 agcgaagccg aacgacatcg cgccgccgcg ccccgggcgg taccgcatgc ggcaccaacg
     3541 aaaacgcggc ccggaggccg cgtcgtcgag gcgtgccggc cgtggcggcc gacgatgccc
     3601 ggatgcctat gcggcgcccg atgttgccgc gtgcgcgacg accagcgccg tcatgttgac
     3661 gatgcggcgc accgtcgacg agggcgtgag cacgtgcacc ggcgcgttgg cgcccagcag
     3721 catcgggccg atcgtgacgc cgtggccgcc ggtcatcttc agcacgttga acaggatgtt
     3781 ggcggcatcg aggttggggc agaccagcac gttggccgag ccggtgagcg tcgaatcggg
     3841 caggatcgcg gcgcgcagct cggggctgaa cgcggcgtcg ccctggatct cgccgtccgc
     3901 cggcacctcg ggcatgcggg cgacgaacag gtcgcgcgcc gcgcgcatct ttctcgcgga
     3961 cggccgttgg ctgctgccga acatcgagtg cgagaggaag gccacgcgcg gctcgagcgc
     4021 gaagcggcgc acctccttca cggccatcgc ggcgatgtcg gcgagctgtt cggcgctcgg
     4081 gtcgtcgttg accgacgtgt cggcgatgaa cagggtgtgc ttgtccagga gcagcgcgtt
     4141 catcgtcgcg aagccgtgcg cgcccgggcg caggccgatg acctcgcgca ggtgcgagaa
     4201 gtggccgtcg aagcggccga ccagcccgca cagcagcgcg tccgcgtcgc ccaggtgcac
     4261 catcagcgcg gcgatcgtcg tgttggagcg gcgcaccgtc gccttggcca tctccgccgt
     4321 gacgccgtcg cgcgcgttca ggcggtgata cgcctcccag tattggcgga agcgcgtgtc
     4381 gtcctcgggg ttgacgacct cgaagtcgcg cccggccttg atgcgcaggc ccgcgcgctc
     4441 gatgcgcgcc tcgatgaccg ccggacggcc gatcaggatc ggcttgacca ggcgctcgtc
     4501 cagcgcgatc tgcaccgcgc gcagcacccg ctcgtcctcg ccctcggcgt aggccacgcg
     4561 cttgggcgcc tggcgtgcgg cgccgaaaag cggccgcatc accagccccg tgtgcgagac
     4621 gaaacggtcg agcgactgca cgtaggcgtc gtgatcggcg atcggccgcg tggcgacgcc
     4681 ggaggcctcg gccgccttcg cgaccgcggg cgcgatgcgc aggatcaggc gcgagtcgaa
     4741 cggcgtcggg atgatgtagt cggggccgaa ccgcagctcg cggccggcat acgcggccgc
     4801 gacctcgccc gagacctcgg ccttcgcgag ctcggcgatc tcgcgcacgc aggcgacctt
     4861 catctcctcc gtgatgcgcg tcgcgccgca gtcgagcgcg ccgcggaaga tgtacgggaa
     4921 gcacaggacg ttgttgacct ggttcgggta gtccgagcgg ccggtggcga cgatgcagtc
     4981 ggggcgcacc gacttcgcga gctcgggccg gatctccggc tcggggttgg cgagcgccag
     5041 gatgatgggc cggtcggcca tcgaggcgac catgtcggcc gtcaccgcgc ccgccgcgct
     5101 gcagccgagg acgacgtcgg cgcccttcat cacgtcggcg agcgtgcgcg cggcggtgac
     5161 ctgctggtag cgcttcttcg attcgtcgag cttgtcctcg cggcggtcgt ggatcacgcc
     5221 cttggagtcg cagacgaaga cgttctcgcg cctgacgccc agcgcgcaca tcaggtcgag
     5281 gcacgcgatg gccgcggcgc cggcgcccga gaccgcgatc ttcacctcgc cgatgcgctt
     5341 gcccaccagc tcgaggccgt tgagcagcgc ggcgctcgag atgatggccg tgccgtgctg
     5401 gtcgtcgtgg aagacgggga tgttcatgcg ctcgcgcagc ttcttctcga tgtagaagca
     5461 ctcgggcgcc ttgatgtcct cgaggttgac gcctcccagc gtcggctcga gcgcggcgat
     5521 gatgtcgacc agcttgtccg ggtcgcgctc ggcgagctcg atgtcgaaca cgtcgatgcc
     5581 agcgaacttc tggaacaggc agcccttgcc ctccatcacc ggcttgcccg cgagcggccc
     5641 gatgtcgccc agtcccagca ccgccgtgcc gttggtgacg acgcccacca ggttgccgcg
     5701 cgccgtgtag tcgtgcgcga gggtcgggtc ctcctcgatc gcgaggcagg cgtaggcgac
     5761 gcccggcgag tacgcgagcg agaggtcgcg ctggttggac agcggcttcg tcggcgtgac
     5821 ggcgatcttg ccgcgcaccg ggctgcggtg gtactcgagg gcggcgtcgc gcagcgcctg
     5881 ctcggcgggc gacagggagg cggtgttctt ttccatggcg ggtcctttcc ggtcggcact
     5941 tcgccggcgg ggaagggatc ttgccagggt tggcgtaacc gcgatgaaac ccgggcgcca
     6001 tcatccttcg cggatggtgg gacgtggctt gcctgcgacg acctcatgcg catccggcct
     6061 cgcgccatgc gggccgtgca tggcgcgcgc cgccgtcgcg gcagcgtcac cggacgtcgg
     6121 tgatcggcgc ggcgctcgcc tcggcgacgc ccagcgcggc gatgcggcgg cagtagtcgt
     6181 gcaggcgctc ggtgtcgcag tggatcacca gcgatcgcac gcggccccag gcgagctcga
     6241 actcgtgcac gccggagttg cagcccacgg tgccgtccgg cagcgtgaag cggtcgtccc
     6301 aggcgacggc cgcgtgcgtg cgccagggcg tgccgccgac cacgatggcg cggatctcga
     6361 acgcgaggcc cggcagcagg cgctgcaggc gctcgtacca gcgcgcggtc gaggccacgg
     6421 tgcggcgctc gcccgccagc gggtggcggc cggccatcgc atggcggtgc tcgcgcgcga
     6481 actggccgac gatgccgtcg tagcggccgg cgttgatgtc ctcgaaggcg cggcgcagct
     6541 tgcggcggac gatggcgcgg tacatggcgc agctcccgtt ggatctgaac actgtcaaga
     6601 tcagcggatg gtggacgcgt atccttctcg tgtcaagatg cggggcatgc ccgcatcccg
     6661 cccgtccgcg aaagtcgcga agcctgccaa gcctgccaag cctgcgaagg gcgcgcccgc
     6721 gccgcgcccg taccaccacg gcgacctgcg cgagagcctg ctcgaggtgg cggcccggtg
     6781 gctcgacgag cgcggcgccg ccacgctcac cctgcgcgag ctggcgaagg cggccggggt
     6841 ctcgcacgcc gcgccgtacc accacttcgc ggggctgga
//
LOCUS       DASVLN010000188         1760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_439700, whole genome shotgun sequence.
ACCESSION   DASVLN010000188 DASVLN010000000
VERSION     DASVLN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439700"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VF453_04300"
     CDS             <1..787
                     /locus_tag="VF453_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7686903.1"
                     /translation="HPAPITPAERSAAHANPGLQRALALAALDLRDEGRREWNYTLRG
                     MSDRELMAAAQWACDSNDWQLCINTSERTKGEVDVLQRYPMPYQRDIVGAARAEGLEP
                     AFVFGLIRQETRFMAQMRSYVGASGLMQLMPSTAKWAARKGGIAYRPELIGDPATNLR
                     IGTFYLKMVMDSFGGSAPMAAAAYNAGPARPRRWRGAANVDPVIWAENVPFLETRDYV
                     KKVMTNAAIYAALQSNRPPLLRARLGPEIGPHDAEQPAAPDLP"
     gene            784..>1760
                     /locus_tag="VF453_04305"
     CDS             784..>1760
                     /locus_tag="VF453_04305"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEX7686904.1"
                     /translation="MKAWIVGGAVRDRLLGEPVNDRDWVVVGASPQDLLDAGYLPVGK
                     DFPVFLHPRTKEEYALARTERKTGPGYKGFAFRAAPDVTLEQDLVRRDLTINAMAQAE
                     DGTLVDPHGGRRDLEARVLRHVSEAFAEDPVRILRLARFAARWPDFSVAPETMALMRG
                     MVAAGEVDALVAERVWQEVSRGLMERAPARMFEVLREAGALARLMPEVDALFGVPQPP
                     VHHPEVDTGVHVMMVLGRCAATAQPLPVRWACLVHDLGKARTPADVLPRHIGHEQRGE
                     RLIRALDERLRVPVECHELALLTAREHTHVHASLGLGAAALMRLLERCDA"
BASE COUNT          239 a          682 c          619 g          220 t
ORIGIN      
        1 gcatcctgcg ccgatcaccc cggccgagcg cagcgccgcc cacgcgaacc cggggctgca
       61 gcgcgcgctc gcgctggcgg cgctggacct gcgcgacgag ggccggcgcg agtggaacta
      121 cacgctgcgc ggcatgagcg accgcgagct catggccgca gcgcaatggg cctgcgactc
      181 caacgactgg cagctgtgca tcaacaccag cgagcgcacc aagggcgagg tcgacgtgct
      241 gcagcgatac cccatgccgt accagcgcga catcgtgggc gcggcgcgcg cggagggcct
      301 ggagccggcc ttcgtcttcg gcctgatccg ccaggagacc cgcttcatgg cccagatgcg
      361 ctcgtacgtc ggcgcgagcg ggctcatgca gttgatgccc tcgacggcca agtgggccgc
      421 gcgcaagggc ggcatcgcct atcgcccgga gctgatcggc gatcccgcga ccaacctgcg
      481 catcggcacc ttctacctca agatggtgat ggacagcttc ggcggctccg cgccgatggc
      541 cgccgcggcc tacaacgccg ggcccgcccg cccgcggcgc tggcgcggcg ccgccaacgt
      601 cgacccggtg atctgggccg agaacgtgcc cttcctcgag acgcgcgact acgtcaagaa
      661 ggtcatgacc aacgccgcga tctacgcggc cctgcagtcc aaccgcccgc cgctgctgcg
      721 cgcgcgcctg ggccccgaga tcggacctca cgacgccgag cagcccgcgg cgcccgacct
      781 gccatgaagg cctggatcgt cggcggcgcc gtgcgcgacc ggctgctggg cgagccggtc
      841 aacgaccgcg actgggtcgt cgtcggcgcg agcccgcagg acctgctgga cgccggctac
      901 ctgcccgtcg gcaaggactt ccccgtgttc ctgcatccgc gcacgaagga ggaatacgcg
      961 ctcgcccgca ccgagcgcaa gaccgggcct ggctacaagg gcttcgcctt ccgcgccgcg
     1021 cccgacgtca cgctggagca ggacctggtg cgccgcgacc tcacgatcaa cgcgatggcc
     1081 caggccgagg acggcacgct cgtcgatccg cacggcggcc ggcgggacct cgaggcgcgc
     1141 gtgctgcgcc atgtctcgga ggccttcgcc gaggatccgg tgcgcatcct gcgcctcgcc
     1201 cgcttcgccg cccgctggcc cgacttcagc gtcgcgccgg agacgatggc gctgatgcgc
     1261 ggcatggtcg cggccggcga ggtcgacgcg ctggtcgccg agcgcgtctg gcaggaggtc
     1321 tcgcgcggcc tgatggagcg cgcccccgcc cgcatgttcg aggtcctgcg cgaggccggc
     1381 gcgctcgcgc ggctgatgcc cgaggtggac gccctcttcg gcgtgccgca gccgcccgtg
     1441 caccatcccg aggtcgacac cggcgtccac gtgatgatgg tgctgggccg ctgcgccgcg
     1501 acggcccagc cgctgccggt gcgctgggcc tgcctcgtcc acgacctcgg caaggcccgc
     1561 acgcccgccg acgtgctgcc gcgccacatc ggccacgagc agcgcggcga gcgcctgatc
     1621 cgcgcgctcg acgagcgcct gcgcgtgccg gtcgagtgcc acgagctcgc gctgctcacc
     1681 gcgcgcgagc acacgcacgt gcacgcgagc ctcggcctcg gcgccgccgc gctcatgcgg
     1741 ctgctcgaac gctgcgacgc
//
LOCUS       DASVLN010000189         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_278427, whole genome shotgun sequence.
ACCESSION   DASVLN010000189 DASVLN010000000
VERSION     DASVLN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278427"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VF453_04310"
     CDS             <1..229
                     /locus_tag="VF453_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX7686905.1"
                     /translation="LVTYGPYLEDDAAVAPSNREFDAFLRARDPASGLRRLADVRDQA
                     AAHGLALRERVAMPANNLMLVFAREPGAATS"
     gene            226..849
                     /locus_tag="VF453_04315"
     CDS             226..849
                     /locus_tag="VF453_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEX7686906.1"
                     /translation="MTDPQDIPDPDELVVARTRAWLRRAVVGLNLCPFARAVDAKGQV
                     RYVVSRAADPEALLATLCVELQRLADTDPAVVDTTLLVHPGVLTDFADFNDFLEVADA
                     AVEDLGLDGVLQVASFHPQFQFADTEPDDVTNATNRAPYPTLHLLREDSVDRAVAAFP
                     EAEAIFERNMERLEALGSEGWAGVQAAWEADARAEVDAANGGPAPGS"
     gene            947..2308
                     /locus_tag="VF453_04320"
     CDS             947..2308
                     /locus_tag="VF453_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HEX7686907.1"
                     /translation="MAPPADTSSIVSLLIVDDNPVQRMQVSALCRELGVPMIYEAGSG
                     REALDLIALLVLPPDLIIVDLEMPAMDGVEFIECLHERGLDVPLIVASSREVVLIQTV
                     ETMARNLGMPVLAALRKPLRSDQLRPAFERWNRERRDDGTRPSGAEPLELPRDELARA
                     IAGGAIKVWYQPKVDMARGKVTGVEALSRWTHPQLGRIGPDRFVALAEREGLIRELTF
                     HVLGTAVAQAARWNALGLPLSLAVNLSPTLLEVPGLVQRIVEMVEREGLPPRQLVLEI
                     TESSVVDCMGAALGVLARLRLQGFGLSIDDYGTGFSSMQQLARIPFSELKIDRSFVHG
                     AHQRTNLRVILQSALTMAHQLGLVTVAEGIETVEDWCLVRDSGCTLGQGWLVAPALAA
                     HELPDWIRAHEPRVLSLGSGQRLAAGATADERRLHERRLYERRMAERRVRGKRAPPPP
                     TSS"
     gene            complement(2310..2816)
                     /locus_tag="VF453_04325"
     CDS             complement(2310..2816)
                     /locus_tag="VF453_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686908.1"
                     /translation="MPHRSLALAAALALAGLACGARAEPAEALKPLAFLAGHCWKGTF
                     ADGQTTDEHCFRWLYDGHVLRDTHVVRAPGRPDAVGESTYYVNPAAGRVEFLYVENAG
                     GISRGTVEALPEALIFPDTQYTGDGEAVVYRARWTPVSPDAYEAWSEAQTPDGWTTMF
                     RIVLRRAD"
     gene            3055..>3367
                     /locus_tag="VF453_04330"
     CDS             3055..>3367
                     /locus_tag="VF453_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686909.1"
                     /translation="MTLDEAIVLFVDLAAARPRAGQEELVAALRARGVEDAARMMALV
                     PMAFAHVVLARAGARLPDDFLICDAGRELTARRRLVDEPIFVAARTLARVMLADDDMR
                     RR"
BASE COUNT          427 a         1235 c         1249 g          456 t
ORIGIN      
        1 cctcgtcacc tacggcccct atctcgagga cgacgcggcc gttgcgccga gcaaccgcga
       61 gttcgacgcc ttcctgcgcg cccgcgaccc ggcctcgggc ctgcgccggc tcgccgacgt
      121 gcgcgaccag gcggccgccc acggcctcgc gctgcgcgaa cgcgtcgcga tgcccgccaa
      181 caacctgatg ctcgtgttcg cccgcgagcc cggagccgcg acctcatgac cgatccgcaa
      241 gacattcccg acccggacga gctcgtcgtc gcccgcaccc gcgcctggct gcgccgcgcc
      301 gtcgtcggcc tgaacctgtg cccgttcgcg cgcgccgtcg acgccaaggg ccaggtgcgc
      361 tacgtcgtca gccgcgccgc cgatcccgag gcgctgctgg ccacgctgtg cgtcgagctg
      421 cagcgcctgg ccgacaccga tcccgccgtc gtcgacacga cgctgctggt acatcctggg
      481 gtattgaccg acttcgccga cttcaacgac ttcctcgagg tcgccgacgc ggcggtcgag
      541 gatctcgggc tggatggcgt gctgcaggtc gcgagcttcc acccgcagtt ccagttcgcc
      601 gacaccgagc ccgacgacgt gaccaacgcg accaaccgcg cgccgtaccc gacgctgcac
      661 ctgctgcgcg aggacagcgt cgaccgcgcc gtggccgcgt tcccggaggc cgaggcgatc
      721 ttcgagcgca acatggagcg actcgaggcg ctcggctccg agggctgggc cggcgtccag
      781 gccgcatggg aggccgacgc gcgggcggag gtggacgcgg caaacggcgg gccggcgccg
      841 ggctcctgac cggcgatgca cggccgcgtg gcggggtcgc cctgcggcgg gcgaggtcgg
      901 gcgaacgagg ctttgcagcg gcgcgcgtcc ggcgcaaact tggcgcatgg ccccgcccgc
      961 ggacacgtct tccatcgtct cgctgctgat cgtcgacgac aacccggtgc agcgcatgca
     1021 ggtctcggcg ctgtgccgcg agctgggcgt gccgatgatc tacgaagccg gcagcggccg
     1081 ggaggcgctg gatctgatcg cgctgctggt gctgccgccc gacctcatca tcgtcgacct
     1141 cgagatgccg gcgatggacg gcgtggagtt catcgagtgc ctgcacgagc gcggactgga
     1201 cgtgccgctg atcgtggcgt ccagccgcga ggtcgtgctg atccagacgg tcgagacgat
     1261 ggcgcgcaac ctcggcatgc ccgtgctggc ggcgctgcgc aagcccctgc gttccgacca
     1321 gctgcgcccg gccttcgaac gctggaaccg cgagcgccgc gacgacggca cgcggccgtc
     1381 cggcgccgag ccgctcgagc tgccgcgcga cgagctggcg cgcgcgatcg ccggcggcgc
     1441 gatcaaggtg tggtaccagc ccaaggtgga catggcgcgc ggcaaggtga cgggcgtcga
     1501 ggcgctctcg cgctggacgc atccgcagct cggccgcatc gggcccgacc gcttcgtcgc
     1561 gctggccgag cgcgaggggc tgatccgcga gctcaccttc cacgtgctgg gcaccgcggt
     1621 cgcgcaggcc gcgcgctgga acgcgctcgg gctgccgctg tcgctggccg tcaacctgtc
     1681 gccgacgctg ctggaggtgc cggggctcgt gcagcgcatc gtcgagatgg tcgagcgcga
     1741 gggcctgccc ccgcgccagc tcgtgctgga gatcaccgag agctcggtcg tcgactgcat
     1801 gggcgccgca ctcggcgtgc tcgcgcggtt gcggctgcag ggcttcggtc tgtcgatcga
     1861 cgactacggc accgggttct cgtcgatgca gcagctcgcg cgcatcccgt tcagcgagct
     1921 caagatcgac cgctccttcg tgcatggcgc gcaccagcgc accaacctgc gcgtgatcct
     1981 gcagtcggcg ctgacgatgg cgcaccagct cgggctcgtg acggtggccg agggcatcga
     2041 gacggtcgag gactggtgcc tggtgcgcga ctcgggttgc acgctcgggc agggctggct
     2101 ggtggccccg gcgctcgccg cgcacgagct gcccgactgg atccgcgccc acgagccgcg
     2161 cgtgctgagc ctgggcagcg gccagcgcct cgcggccggc gcgaccgccg acgagcgccg
     2221 gctccacgaa cgccgcctct acgagcgccg catggccgag cgccgcgtgc gcggcaagcg
     2281 cgcgccgccg ccgccgacat cctcgtgact cagtcggcgc gccgcagcac gatccggaac
     2341 atcgtcgtcc agccgtccgg cgtctgcgcc tcgctccagg cctcgtaggc gtccggggag
     2401 acgggcgtcc agcgggcacg gtagacgacc gcctcgccgt cgccggtgta ctgcgtgtcg
     2461 ggaaagatca gcgcctcggg cagcgcctcc acggtgccgc ggctgatgcc gccggcgttc
     2521 tcgacgtaga ggaactcgac gcggcccgcc gccgggttga cgtaataggt cgattcaccg
     2581 accgcgtccg gccggcccgg ggcgcgcacg acgtgcgtgt cgcgcaggac atggccatcg
     2641 tagagccagc ggaagcagtg ttcgtcggtg gtctgtccgt ccgcgaaggt gcccttccag
     2701 caatggccgg cgaggaaggc gagcggcttc agggcctccg ccggctcggc gcgggcgccg
     2761 caggcgaggc cggccagggc gagggcggcg gcgagtgcga gggagcggtg cggcatggag
     2821 ggcagtcctg cgggctttcg gggaacgcgg tcggtgcccg ccggcgcccc tgccgccgac
     2881 gggtgccgcg cagtctatcg acaacaggcg gcgcgggctc tcccgccaaa gggggaggag
     2941 cgcgctatcc tgccgccatg tcctccagcg cgcgttccgc atgaagcacg ccgggcccgc
     3001 catgctcgcc acgccggggc cgctgcgcgc gcgcccgggc gtcgcatccg cgcgatgacg
     3061 ctggacgaag ccatcgtcct gttcgtcgac ctcgccgcgg cgcgcccgcg ggcggggcag
     3121 gaggagctgg tcgccgcgct gcgggcgcgg ggcgtcgagg acgccgcacg catgatggcg
     3181 ctggttccca tggcgttcgc gcacgtggtg ctcgcgcgcg cgggggcgcg gctgccggac
     3241 gacttcctga tctgcgacgc cggccgcgag ctcaccgcgc gccggcggct ggtcgacgag
     3301 ccgatcttcg tcgcggcgcg caccctcgcg cgggtgatgc tggccgacga cgacatgcgc
     3361 cgccgcg
//
LOCUS       DASVLN010000190         4257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_332014, whole genome shotgun sequence.
ACCESSION   DASVLN010000190 DASVLN010000000
VERSION     DASVLN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332014"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VF453_04335"
     CDS             <1..524
                     /locus_tag="VF453_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX7686910.1"
                     /translation="HSRAALVWCVASADDPVAPLTDAQRLAVLAHRLPDVVGTPVAIS
                     PLKSFALGLNAEASVVDGRVVRIGNAAQTLHPVAGQGLNLGLRDAFLLVDRLADGARA
                     SDHGVARALRTLAFARAPDRWSVIGATDFLARSFTWQAPGLAAARGLGLAALQALPPL
                     KRAFARQMMFGWR"
     gene            582..1514
                     /locus_tag="VF453_04340"
     CDS             582..1514
                     /locus_tag="VF453_04340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEX7686911.1"
                     /translation="MFVVAGEALMDVFTGATTPSGIALDARIGGSPLNVAFGLARMGQ
                     HVAFLGGISRGELGERLMDALRAEGVSLDCVHRSHAPTTISLIGVDARGVPEYAFYGA
                     GAADRTLPLEALERLPADVRALHVGSYTMVTEPTASTHRALIERVHGRVLVSYDPNLR
                     LNVEPDIEVWRARLEWMLPHADVLKLSEEDLHLLYPGADPVHFAHECLAKGAGLVALT
                     RGGEGAMAWHSSGSVEVPPVHVDVIDTVGAGDTFQAALLTRLEELHVLSPAGVRGMSG
                     KLLLDAMRFAAEAAAITCSRRGADLPRRAELGIR"
     gene            complement(1534..2172)
                     /locus_tag="VF453_04345"
     CDS             complement(1534..2172)
                     /locus_tag="VF453_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686912.1"
                     /translation="MGLGIDVGYLANMIASDPEGAEWFEDEMASVNRVLAAEGLPAHV
                     EPRIAPPAGRCRTTGFPYSTLHCLRRAYAHRVADPGWTATAVPEGVDPADDDEVEAQT
                     ERFDSHLLCHSDSEGFYLPIDFPQVVFANEDDDIPGGLLGSTQRLRAELALVAPALGV
                     ALDADGELADEEAARLDAIDTDHPLRRELLAWFALWEAARRSLASGCAIVFC"
     gene            complement(2249..2761)
                     /locus_tag="VF453_04350"
     CDS             complement(2249..2761)
                     /locus_tag="VF453_04350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7686913.1"
                     /translation="MSASSADLTIRPATAADAAPLRAIRNHWIAASHASFDEQPLAPE
                     AVRDWIAGFASTGPHRLLVAERAGVVVGFASSQPYRAHPAFRHTVETSIYVAAGAGRG
                     GIGSRLYDALFAAIADEGLHRAVVGIALPNEASVRLHRRQGFREVGTFDEYAIKNGRF
                     ISSVWMQRTL"
     gene            complement(2802..3728)
                     /locus_tag="VF453_04355"
     CDS             complement(2802..3728)
                     /locus_tag="VF453_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7686914.1"
                     /translation="MNTPEPRVLAEMKSLVEGFASADGMTTSPLPGVSFGRSGASLPR
                     SPVLIDPCLVLVVQGRKRSYLGDQVFDYGPGQYLVASMPMPFEGESFATPEEPLLVVK
                     VKLDLALVGELALALDAGAESSVAPEGMFAAAVDDCIADAVVRLLRALRSPLETRLFG
                     QALVRELCFRVLTGERGAALRAGLAQRGHFGQIAKALQRIHVDYPQRLDIDTLAREAN
                     LSAAAFHAHFKTVTATSPIQYLKATRLHKARLLMIQDGVSASRASSRVGYESASQFSR
                     EFKRLFGRTPVEELRLVHATYAPQPQEARDRP"
     gene            3970..>4257
                     /locus_tag="VF453_04360"
     CDS             3970..>4257
                     /locus_tag="VF453_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686915.1"
                     /translation="MTSHVIAVCTAPGKTDLRAGRPLPRAADSPRRSSAPTVPVRPLS
                     QPAPVADRARDAPPSRDAHPEEKKMRKTWFVTGAASHVGAELVRDALAAGHA"
BASE COUNT          586 a         1558 c         1533 g          580 t
ORIGIN      
        1 gacactcgcg ggcggcgctc gtgtggtgcg tcgccagcgc ggacgacccg gtcgccccgc
       61 tcaccgacgc ccagcgcctg gccgtgctcg cgcaccgcct gccggacgtg gtcggcacgc
      121 cggtcgcgat ctcgccgctg aaatccttcg ccctcggcct gaacgccgag gccagcgtgg
      181 tcgacggccg cgtcgtgcgc atcggcaacg cggcgcagac gctgcatccg gtggccggcc
      241 agggcctgaa cctggggctg cgcgacgcgt tcctgctggt cgaccgcctc gccgacggcg
      301 cgcgcgcgtc cgaccatggc gtcgcacgcg cgctgcgcac gctcgcgttc gcgcgcgcgc
      361 ccgaccgctg gagcgtcatc ggggcgacgg acttcctcgc gcgcagcttc acgtggcagg
      421 caccgggcct cgcggccgca cgcgggctgg ggctcgcggc gctgcaggcg ctgccgccgc
      481 tcaagcgcgc attcgcgagg caaatgatgt tcggatggcg gtgagaaccc aaggtcgcgc
      541 aagcggcagc aactcagcaa cgacgagaaa gtagacacct catgttcgtg gtcgcgggcg
      601 aagccctgat ggatgtgttc accggcgcga cgacgccgtc cggcatcgcg ctcgacgcgc
      661 gcatcggcgg ctcgccgctg aacgtggcgt tcgggctcgc gcgcatgggg cagcacgtgg
      721 ccttcctcgg cgggatctcg cgcggcgagc tcggcgagcg cctgatggac gcgctgcgcg
      781 ccgaaggcgt gtcgctcgac tgcgtccacc gcagccatgc gccgaccacg atcagcctga
      841 tcggcgtcga tgcgcgcggc gtcccggagt acgcgttcta cggcgccggc gccgccgacc
      901 gcacgctgcc gctggaggcg ctcgagcgcc tgcccgccga cgtgcgggcg ctgcacgtcg
      961 gctcgtacac gatggtcacc gagcccaccg cctcgacgca ccgggcgctg atcgagcgcg
     1021 tgcacggccg ggtgctcgtg agctacgacc cgaacctgcg cctgaacgtc gagcccgaca
     1081 tcgaggtctg gcgcgcccgc ctcgagtgga tgctgccgca cgccgacgtg ctcaagctca
     1141 gcgaggagga cctgcacctg ctctaccctg gcgccgaccc cgtgcacttc gcgcacgaat
     1201 gcctcgccaa gggcgccggc ctcgtcgcgc tgacccgcgg cggcgagggc gcgatggcct
     1261 ggcattcgag cggcagtgtc gaggtgccgc cggtgcacgt cgacgtcatc gataccgtcg
     1321 gcgcgggcga caccttccag gccgcgctgc tgacgcggct ggaggagctg cacgtgctct
     1381 cgcccgccgg ggtgcgcggc atgtcgggca agctgctgct cgacgcgatg cgcttcgccg
     1441 ccgaggccgc ggccatcacc tgctcgcgcc gcggcgccga cctgccgcgg cgggccgagc
     1501 tgggcatccg ctgaccggcc ggcgcaggca gcgtcagcag aagacgatcg cgcagccgct
     1561 cgccaggctt cgccgcgccg cctcccacag cgcgaaccag gcgagcagct cgcgccggag
     1621 cgggtggtcg gtgtcgatcg cgtccaggcg cgccgcctcc tcgtcggcca gctcgccgtc
     1681 ggcgtcgagc gcgacgccca gtgccggcgc cacgagcgcg agctccgcgc gcagccgctg
     1741 cgtcgagccc agcaggccgc cggggatgtc gtcgtcctcg ttcgcgaaca cgacctgcgg
     1801 gaagtcgatc ggcaggtaga agccctcgct gtccgagtgg cacagcaggt ggctgtcgaa
     1861 ccgctccgtc tgcgcctcga cctcgtcgtc gtcggccgga tcgaccccct cgggcacggc
     1921 ggtcgccgtc cagccggggt ccgcgacgcg gtgcgcgtag gcgcggcgca ggcaatgcag
     1981 cgtcgagtac gggaagcccg tcgtgcggca tcgacccgcc ggcggggcga tgcgcggctc
     2041 gacgtgcgcg ggcaagcctt ccgcggcgag cacgcggttg acgctcgcca tctcgtcctc
     2101 gaaccactcg gcgccctcgg gatcactggc gatcatgttg gcgaggtagc cgacgtcgat
     2161 gccgaggccc atgccgtgtc ctttcattgc aagtgacgat cttgcgattg tggcccgggc
     2221 aagtgtcggc gccgcggccg gcgacggttc aaagcgtccg ctgcatccac accgagctga
     2281 tgaagcggcc gttcttgatc gcgtactcgt cgaaggtgcc gacctcgcgg aagccctggc
     2341 ggcggtgcag gcgcaccgag gcctcgttgg gcagcgcgat gccgaccacc gcgcggtgca
     2401 ggccctcgtc cgcgatcgcg gcgaacaagg cgtcgtacag gcgcgagccg atgccgccac
     2461 ggcccgcgcc cgcggcgacg tagatgctcg tctcgacggt gtggcggaac gccggatgcg
     2521 cgcgataggg ctggctgctc gcgaagccga cgaccacgcc ggcgcgctcc gcgacgagca
     2581 gccggtgcgg cccggtcgac gcgaagcccg cgatccagtc gcgcacggcc tcgggcgcga
     2641 gcggctgctc gtcgaagctg gcgtgggacg cggcgatcca gtggttgcgg atcgcgcgca
     2701 ggggcgcggc gtcggcggcc gtcgcagggc ggatggtcag gtcggcggac gaggcgctca
     2761 tgccaccgat tgtggcgcgg cgtcgggacg cggccgccgc gtcacggccg gtcgcgggct
     2821 tcctgcggct gcggcgcgta ggtcgcgtgc acgaggcgca gctcctcgac cggcgtgcgc
     2881 ccgaacaggc gcttgaactc gcggctgaac tgcgacgcgc tctcgtagcc gacgcggctg
     2941 gacgcgcggc tcgcgctcac gccgtcctgg atcatcagca gccgcgcctt gtgcagccgc
     3001 gtcgccttca ggtactggat cggcgaggtc gcggtgaccg tcttgaaatg cgcgtggaaa
     3061 gccgcggcgc tcaggttggc ctcgcgcgcg agggtgtcga tgtccaggcg ctgcggatag
     3121 tcgacgtgga tgcgctgcag cgccttggcg atctggccga aatgcccgcg ctgcgcgagc
     3181 ccggcgcgca gcgcggcgcc gcgctcgccc gtgagcacgc gaaagcacag ctcgcgcacg
     3241 agcgcctggc cgaacagccg cgtctccagc ggcgagcgca gcgcgcgcag caggcgcacg
     3301 acggcgtcgg cgatgcagtc gtcgaccgcg gcggcgaaca tgccctccgg cgcgaccgag
     3361 gactcggcgc ccgcatccag cgccagcgcc agctcgccca ccagcgcgag gtcgagcttg
     3421 accttgacca ccagcagcgg ctcctcgggc gtcgcgaacg actcgccctc gaacggcatc
     3481 ggcatcgacg cgaccaggta ctggcccggc ccgtagtcga acacctggtc gcccaggtag
     3541 ctgcgcttgc ggccctggac gacgagcacc aggcaggggt cgatcagcac cggcgagcgc
     3601 ggcagcgacg cgcccgaacg cccgaaggag acccccggca gcggcgaggt cgtcatgccg
     3661 tccgccgacg cgaagccttc gacgagcgat ttcatctccg cgagcacgcg cggctcgggc
     3721 gtgttcatgt ccgacgccat cctgtcatgt gagcgcatta taggaaaggg acagcttctt
     3781 gggctgttgg cgctacgaat agtcgtagaa catagtttta ggcaagaagt ctgtagcttc
     3841 cgatctttcg gacgccccca cggatttcct aagatgcacg gcacccggcc tcgaagagag
     3901 ctggcgtgcg cggcccggac aagccgcctt gcgcgatccg cctcgccttg cggcgacggc
     3961 agtaccgcca tgacctcgca cgtcattgcc gtctgcaccg cgcccgggaa gactgatctc
     4021 cgtgcaggcc gcccgctccc gcgggcagcg gactccccgc gcaggagctc cgccccgacc
     4081 gttcccgttc gaccgctctc gcagccggcg cccgtcgccg accgcgcgcg cgatgcgccg
     4141 ccctcgcgcg acgcccaccc cgaggagaag aagatgagaa agacttggtt cgtgaccggt
     4201 gccgcttccc acgtcggcgc agagctcgtg cgcgacgcgc tggcggccgg ccacgcc
//
LOCUS       DASVLN010000191         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_62861, whole genome shotgun sequence.
ACCESSION   DASVLN010000191 DASVLN010000000
VERSION     DASVLN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62861"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VF453_04365"
     CDS             <1..733
                     /locus_tag="VF453_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7686916.1"
                     /translation="APVAAPAPAPASAQEPAPAADAGDQESFIYSVSHDLRAPIRVVE
                     GFARILKEDYGRFLDRIGNDHVDRILAAAARMNSMIDALLALSRLQSQPLKRQPVDLS
                     QLARYVVDDLRGNEPDREADIHIEPGLVVHGDPTLIRIAVENLVGNAWKYSAQAPRTR
                     IELRREDVNGQPAFVVADAGAGFDMRFADRLFGVFQRLHSAKDFQGTGVGLASVRRIV
                     RRHGGDIWAESEVGKGARFYFTLPS"
     gene            832..1311
                     /locus_tag="VF453_04370"
     CDS             832..1311
                     /locus_tag="VF453_04370"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX7686917.1"
                     /translation="MTLTLSDLLATGDRWIACLPATPDDIARLESELGLRVPGELRAA
                     LLASNGLKLPHPYLDDPESAMLGLLDVDGIIDATRQFRAHLLDGERPWRADVAPGRLL
                     LIAETVFGDALAYLCDDADPSAATLVQIHQDHRDGDSLDPLKGLTLLDLLADLGEED"
     gene            complement(1312..>2370)
                     /locus_tag="VF453_04375"
     CDS             complement(1312..>2370)
                     /locus_tag="VF453_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7686918.1"
                     /translation="VPAAAVQRALAGSAAGMASRCPPQGLPDLRERIALMLRGQGIPV
                     DAGRVLTTYGGTHAIDMICRTFLQPGDAVVVEDPGYFLMFGRLQQEGVRIVPVARRRD
                     GIDIEQLEAACVEHKPRLAFLQTVLHNPTGWGSSVANLHKVLMLAHKHGFLVAEDDVQ
                     GHFHPGQATRLAALAGMERVIYYSSFCKALSPALRVGYVAADPVLLKPLLRHKIHTVL
                     TTAALNEYVMLEVLAAGRLRKHLDRLNRRLGDARRTSEAQLAAAGIAFESPGEGGLFL
                     WGEVPAGVDVDQLVQDAMRDKILLVRGAAFMADAAGDPHIRFNAAFCRDPRVADWLVQ
                     RFAATSGASELVRRAMAR"
BASE COUNT          327 a          919 c          813 g          311 t
ORIGIN      
        1 cgccccggtg gccgcaccgg cgcccgcacc cgcctccgcg caggagcccg cgccggccgc
       61 ggacgcgggc gaccaggagt cgttcatcta ctccgtctcg cacgacctgc gcgcgccgat
      121 ccgcgtcgtc gagggcttcg cccgcatcct caaggaggac tacggccgct tcctcgaccg
      181 catcggcaac gaccacgtcg accgcatcct cgccgcggcg gcgcgcatga acagcatgat
      241 cgacgcgctg ctcgcgctct cgcgcctgca gtcgcagccg ctcaagcgcc agccggtgga
      301 cctgtcgcag ctcgcgcgct acgtcgtcga cgacctccgc ggcaacgagc ccgaccgcga
      361 ggccgacatc cacatcgagc ccggcctcgt cgtgcacggc gacccgacgc tgatccgcat
      421 cgcggtggag aacctcgtcg gcaacgcgtg gaagtacagc gcgcaggcgc cgcgcacgcg
      481 catcgagctg cggcgcgagg acgtcaacgg ccagccggcc ttcgtcgtcg ccgacgccgg
      541 cgccggcttc gacatgcgct tcgcggaccg cctgttcggc gtgttccagc gcctgcacag
      601 cgccaaggac ttccagggca cgggcgtcgg cctcgcgtcg gtgcgccgca tcgtgcggcg
      661 ccacggcggc gacatctggg ccgagtccga ggtcggcaag ggcgcgcgct tctatttcac
      721 gctgccgtcc tgaaatcctg ccgaatttcc gggactcgac cgccgcggac gcgctagagt
      781 ccgggcagac cgccgcgact ttcccgcggc ccaacgcccg ccccgccccc cgtgaccctg
      841 acgctgtccg acctcctcgc caccggcgac cgctggatcg cctgcctgcc cgcgacgccc
      901 gacgacatcg cgcgcctcga atccgagctc ggcctgcgcg tgccgggcga gctgcgcgcc
      961 gcgctgctcg cgtccaacgg cctgaagctg ccgcatccct acctcgacga ccccgagtcc
     1021 gcgatgctgg gcctgctgga cgtcgacggc atcatcgacg ccacgcgcca gttccgcgcc
     1081 catctcctcg acggcgagcg tccgtggcgc gccgacgtcg cgcccgggcg cctgctgctg
     1141 atcgccgaga ccgtcttcgg cgacgcgctc gcctacctct gcgacgacgc cgacccgtcc
     1201 gccgccacgc tggtgcagat ccaccaggac caccgcgacg gcgactcgct cgacccgctc
     1261 aaggggctga cgctgctgga cctgctggcc gatctcggcg aggaagactg atcagcgcgc
     1321 catcgcgcgc cgcacgagct cgctcgcgcc cgaggtcgcc gcgaagcgct gcaccagcca
     1381 atcggccacg cgcgggtcgc ggcagaacgc ggcgttgaag cggatgtgcg gatcgccggc
     1441 ggcgtcggcc atgaaggcgg cgccgcgcac cagcaggatc ttgtcgcgca tcgcgtcctg
     1501 cacgagctgg tcgacgtcga cgcctgcggg cacctcgccc cacaggaaca ggccgccctc
     1561 gccgggcgac tcgaacgcga tgcccgcggc ggcgagctgc gcctcgctgg tgcggcgggc
     1621 gtcgcccagg cgccggttca ggcggtcgag gtgcttgcgc aggcggccgg cggcgagcac
     1681 ctccagcatc acgtactcgt tgagcgccgc ggtcgtcagc acggtgtgga tcttgtggcg
     1741 cagcaacggc ttgagcagca ccgggtcggc cgcgacgtag cccacgcgca gcgccgggct
     1801 gagcgccttg cagaagctcg agtagtagat cacccgctcc atgccggcca gcgccgccag
     1861 gcgcgtcgcc tggccaggat ggaagtggcc ctggacgtcg tcctcggcca cgaggaagcc
     1921 gtgcttgtgc gcgagcatca gcaccttgtg caggttcgcg acgctcgagc cccagccggt
     1981 cgggttgtgc agcacggtct gcaggaaggc gaggcgcggc ttgtgctcga cgcacgccgc
     2041 ctcgagctgc tcgatgtcga tgccgtcgcg ccggcgcgcc accggcacga tgcgcacgcc
     2101 ctcctgctgc aagcggccga acatcaggaa gtagccggga tcctcgacca ccaccgcgtc
     2161 gccgggctgc aggaaggtgc ggcagatcat gtcgatcgcg tgcgtgccgc cgtaggtcgt
     2221 gagcactcgc ccggcgtcga ccgggatgcc ctggccgcgc agcatcagcg cgatgcgctc
     2281 gcgcaggtcg ggcagcccct gcggcgggca acgcgaggcc atgcccgcgg cgctgccggc
     2341 gagcgcgcgc tgcaccgcgg cggcaggcac
//
LOCUS       DASVLN010000192         8611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_439948, whole genome shotgun sequence.
ACCESSION   DASVLN010000192 DASVLN010000000
VERSION     DASVLN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439948"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2227)
                     /locus_tag="VF453_04380"
     CDS             complement(<1..2227)
                     /locus_tag="VF453_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7686919.1"
                     /translation="MPPPSSAIDTEQFERIARRNVTLPLVAGVVSAAAFVAFFVYFLQ
                     VMGWVDHTHLVIGRFNELAALEADVEASSRAYLLTGDEANLGPYNVAKTQLDGQIDDL
                     AKLTEDNPTQTDRLKRVRTTQHAYEDFAQQMINLRRNKADFSSLLGSQLGRQLSDRIR
                     GELVAGKDAEQGLLTDRLDSAHTVTWAGIGSYLLFIFVVFSFIAYAGRRDIRQLSTDF
                     GAALKAQTDAAEDLQRQAWVLDGQAQLAQSLVGQQTLATIGRSVLQFLAGTLGPVVAA
                     MYVRDANGRMARVSTHGFDKALEAREDDPEAGLVAQAARDARINHVRGLTGSYFKVAS
                     GLGETVPAEIVVAPVTNDGVVNGVLELGFMRPVTPRDLQLLKLVEGSIGASVEAAQYR
                     KRLQDAVEETQSLNEELQVQQEELRTANEELGEQSRVLTESQQHLEHQQAELEATNTQ
                     LAEQAHVLDRRNEELAQAQKVLEERADELQRASRYKSEFLANMSHELRTPLNSSLILA
                     KLLSENKEGNLNPEQLKFAQTIYSAGNDLLNLINDILDLSKVEAGRLELQPQFVSVPR
                     LVESLERTFAPLAQDKRLAFEVRVAPEAPSSLVTDNQRVEQILKNLLSNAIKFTDKGG
                     VSVHVEPRPDDRIAFSVRDSGIGIPESQQEIIFEAFRQADGTINRRYGGTGLGLSISR
                     ELARLLGGAITVESAAGQGSAFTLTLPVKWTDVPDQEPAGAPSPRAAGVLAPVQSPRA
                     LP"
     gene            complement(2419..3687)
                     /gene="hemW"
                     /locus_tag="VF453_04385"
     CDS             complement(2419..3687)
                     /gene="hemW"
                     /locus_tag="VF453_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX7686920.1"
                     /translation="MNVIPIDPAPGEGDAAGPPERDPQWYHRPGTLALPAPPPLAVYV
                     HLPWCLRKCPYCDFNSHEFRTAGATPADLPETAYLDALRADLERALPLVWGRQVHSVF
                     IGGGTPSLFSPAGIERLVSDLRMLLPLAGDCEITMEANPGTFEKDRFRAFAQAGVTRL
                     SIGVQSFDDAQLAALGRVHDRAQALAAAEEAARSFGTFNLDLMYALPGQSLAGLEADL
                     AQALALAPPHLSVYHLTMEPNTVFGRRPPANLPDDDLASDMLDLITARTAGAGLERYE
                     VSAYALPGHRCRHNLNYWQFGDYLGLGAGAHGKLSFAHRVVRQVRWREPRMYIERARA
                     GRAMSNEADVARAQLPFEFMLNALRLREGFALALFTQRCGLPLSAIEPALAEGERRGL
                     LERGGPPGGEWLRPSARGFDFLNDLTELFL"
     gene            complement(3684..4328)
                     /locus_tag="VF453_04390"
     CDS             complement(3684..4328)
                     /locus_tag="VF453_04390"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX7686921.1"
                     /translation="MKLVLASNNAKKLAELQALFDGLGVALVRQGELGVSEAEEPHVT
                     FIENALAKARHASAATGLPAIADDSGLCVDALGGAPGVVSANYAVVAASAGRALPREV
                     VRETQDAANNALLLERMAAAEHRHAAFVCTLVAIRAADDPQPLVAVGRWEGEILSALE
                     GQGGFGYDPLMYIPALGATVAQLAPEVKNAHSHRALAAARMRELMRAAWRLQGR"
     gene            complement(4352..5083)
                     /gene="rph"
                     /locus_tag="VF453_04395"
     CDS             complement(4352..5083)
                     /gene="rph"
                     /locus_tag="VF453_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7686922.1"
                     /translation="MAFQRSGARAANELRPVRLTRRFTAHAEGSVLVEFGGTRVLCTA
                     SVEERVPPFRKGSGEGWVTAEYGMLPRATHTRSDREAAKGKQSGRTQEIQRLIGRSLR
                     AVFDLKLLGERTITLDCDVLQADGGTRTAAITGACVAAHDAVATLLAAGKIERTPLTG
                     FVAAVSVGIVEGTPLLDLEYVEDSACDTDMNVVMTGAGHFIEIQGTAEGAPFSRIEMN
                     QMLDLADGGIRQLIAAQKSALGLAA"
     gene            complement(5169..6083)
                     /locus_tag="VF453_04400"
     CDS             complement(5169..6083)
                     /locus_tag="VF453_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX7686923.1"
                     /translation="MRFGTYQVSRKGGREKNEDRMGYCYTRDSGLFALADGMGGHPEG
                     EVASQLALQTLAAYFQKEAKPDLKDPQRFLQDAILAGHHQLLRYATDRGLTDTPRTTV
                     VACVLQGGSIYWAHCGDSRLYLMRGDRLIARTRDHSYSELREALSHVVPIEEKVNRNV
                     LFTCLGSPGKPVIDSAGPMVLRAGDRVLLCSDGLWSSVEDTDIVNLMATYPLHDSVPE
                     LVEQALRNAGEKSDNVTALAVEWEGSEDEDRSGGVSTRSLGEDVFASTIQASMTGDSF
                     LPDIDDDEIERSIREINEAIQRSGPRKT"
     gene            complement(6173..7141)
                     /locus_tag="VF453_04405"
     CDS             complement(6173..7141)
                     /locus_tag="VF453_04405"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7686924.1"
                     /translation="MSKPKPAPLPSGTVVGGYQIVKKIAAGGFGIVYLAEDETRHLVA
                     LKEYLPASLADRSPGELIPRVKPEKQPLYRLGLKSFFEEGRSLAQISHASVVSVLNFF
                     RENETVYMVMNYLQGDTLQDFIVTARDLKREKVLRESTIRSLFDEILRGLRIVHQHKM
                     LHLDIKPANIFITNDNKAVLLDFGAAREVLSKEGQFIRPMYTPGFAAPEMYRRDGSLG
                     PWTDIYAIGACLYACMQGYPPNDAPQRIDKDRLPLSLSRLRNVYSDNLIEVIEWCMAL
                     DPLSRPQSVFALQKELSREIERRYTTLSFSERLKLQIENLKSGAHP"
     gene            7172..8104
                     /locus_tag="VF453_04410"
     CDS             7172..8104
                     /locus_tag="VF453_04410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX7686925.1"
                     /translation="MAVYSMTGFGNASATAASAASTEGAAPAAPADSAAVTVELRSVN
                     SRFLDLAFRMPDELRQHEGALRELVSAACRRGKVDIRVSAARTSGDDWPQPTPDQLNR
                     LSRLEGAVTAWMPKAAPLSVNEVLHWCRSAAPAPAARMDEALLDAARRAVAALKEARA
                     REGDKLVAILRERIVRLRELAAQAEPLVPAVVERQQQRFLERWNDALKSTGSADVPTD
                     ALRERALNEAATFALRIDVAEELSRLRAHLEEIDRLLSAGGELGKRLDFLIQELHREA
                     NTLGSKSAALELTGVSVEMKVLIEQMREQVQNVE"
     gene            8135..>8611
                     /locus_tag="VF453_04415"
     CDS             8135..>8611
                     /locus_tag="VF453_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686926.1"
                     /translation="MRPAVPAGGTPTAAIDDDGRVRRSCAPSSTTHRSMPRSDIRFRR
                     TGTLAAVVLLTVGAAHGQAKDHAVDVWPAAPAPAIAPESILAHQDVAALGRQLQVGDI
                     VFTRVGAYPFRKVADATGTWTNHVGIVLSTAGPEPVLGESRFPFSGTTPFARFVARS"
BASE COUNT         1195 a         3011 c         3087 g         1318 t
ORIGIN      
        1 cgggcagcgc gcgcggcgat tgcaccggcg cgaggacgcc ggcagcacgg ggcgacggcg
       61 cgcccgcggg ctcctggtcg ggcacgtcgg tccacttcac gggcagcgtc agcgtgaacg
      121 cgctgccctg gccggcggcg ctctccaccg tgatcgcccc gcccaacagc cgcgcgagct
      181 cgcgcgagat cgacagcccg agccccgtgc cgccgtagcg gcggttgatc gtgccgtcgg
      241 cctggcggaa ggcctcgaag atgatctcct gctggctctc ggggatgccg atgcccgagt
      301 cccgcaccga gaacgcgatc cggtcgtcgg gccgcggctc gacgtgcacc gacacgccgc
      361 ccttgtcggt gaacttgatg gcattggaca gcaggttctt caggatctgc tcgacgcgct
      421 ggttgtcggt gacgagggag ctcggcgcct cgggtgcgac gcgcacctcg aaggcgagcc
      481 gcttgtcctg ggcgagcggc gcgaaggtgc gctcgagcga ctccaccagc cgcggcaccg
      541 agacgaactg cggctgcagc tcgagccggc cggcctccac cttcgagagg tcgaggatgt
      601 cgttgatcag gttcagcagg tcgttgccgg cgctgtagat cgtctgcgcg aacttcagct
      661 gctcggggtt gaggttgccc tccttgttct cggacagcag cttggcgagg atcagcgagc
      721 tgttgagcgg cgtgcgcagc tcgtgcgaca tgttggcgag gaactcggac ttgtagcggc
      781 tcgcgcgctg cagctcgtcg gcgcgttcct cgaggacctt ctgcgcctgg gcgagctcct
      841 cgttgcggcg atcgagcacg tgggcctgct cggcgagctg cgtgttcgtg gcctcgagct
      901 cggcctgctg gtgctcgagg tgctgctgcg actccgtgag cacgcgcgac tgctcgccga
      961 gctcctcgtt ggcggtgcgc agctcctcct gctgcacctg cagctcctcg ttgagggact
     1021 gcgtctcctc gaccgcgtcc tgcaggcgct tgcggtactg cgcggcctcg accgacgcgc
     1081 cgatcgaccc ctccaccagc ttcagcagct gcaggtcgcg cggcgtcacg ggtcgcatga
     1141 agccgagctc gaggacgccg ttgacgacgc cgtcgttggt caccggcgcg acgacgatct
     1201 cggccggcac cgtctcgccg aggccgctgg cgaccttgaa gtagctgccc gtcaggccgc
     1261 gcacatggtt gatccgcgca tcgcgcgccg cctgcgcgac gaggccggcc tcgggatcgt
     1321 cctcgcgcgc ctccagcgcc ttgtcgaagc cgtgggtcga cacgcgcgcc atgcggccgt
     1381 tggcgtcgcg cacgtacatc gctgccacca cggggccgag ggtgccggcc aggaactgca
     1441 gcacgctgcg gccgatcgtc gcgagcgtct gctggccgac cagcgactgc gcgagctggg
     1501 cctggccgtc cagcacccag gcctgccgct gcaggtcctc ggccgcgtcg gtctgggcct
     1561 tcagcgcggc gccgaagtcg gtcgacagct ggcggatgtc gcggcgcccc gcatacgcaa
     1621 tgaacgagaa gacgacgaag atgaacagca ggtacgagcc gatgcctgcc caggtcaccg
     1681 tgtgcgcgga gtccaggcga tcggtcagca ggccctgctc cgcgtccttg cccgcgacca
     1741 gctcgccgcg gatgcggtcg ctgagctggc gtccgagctg cgagcccagc agcgagctga
     1801 agtccgcctt gttgcgccgc aggttgatca tctgctgggc gaagtcctcg tacgcgtgct
     1861 gcgtcgtgcg cacgcgcttg aggcggtcgg tctgcgtcgg gttgtcttcc gtcagcttcg
     1921 ccaggtcgtc gatctggcca tccagctgcg tcttggcgac gttgtagggg ccgaggttgg
     1981 cctcgtcgcc cgtcagcagg taggcgcggc tgctcgcctc gacgtcggcc tccagggccg
     2041 cgagctcgtt gaagcggccg atgacgaggt gagtgtggtc gacccagccc atcacctgca
     2101 ggaagtagac gaagaaggcg acgaacgcgg cggcgctgac gacgccggcg accagcggca
     2161 gcgtgacgtt gcggcgggcg atccgttcga actgctcggt gtcgatggcg gacgagggtg
     2221 gcggcatggt gtcccgttcg tgcggcggca gggtccgtcc ctgctcggcc gttcgggcct
     2281 gcgagcaaga accgatcccg cgctgcagcc cgcatggata cggggcgcgc gggcctttcc
     2341 ccgatctcgc ccagcggcga tcggtaacga ttaccgatgc cgttcccgat cgccggcaag
     2401 cccttccggc ccggtcgttc agaggaacag ctcggtgagg tcgttgagga aatcgaagcc
     2461 gcgcgcgctc ggtcgcagcc attcgccgcc cggcggcccg ccgcgttcga gcaggccgcg
     2521 gcgttcgcct tcggccagcg ccggctcgat cgccgacagc ggcaggccgc agcgctgcgt
     2581 gaacagcgcg agcgcgaagc cctcgcgcag gcgcagcgcg ttgagcatga actcgaatgg
     2641 cagctgggcg cgcgcgacgt cggcctcgtt ggacatcgcg cgcccggccc gcgcgcgctc
     2701 gatgtacatg cgcggctcgc gccagcgcac ctggcgcact acgcggtgcg cgaagctcag
     2761 cttgccgtgc gccccggcgc ccaggcccag gtaatcgccg aactgccagt agttcaggtt
     2821 gtgccggcag cgatggccag gcagcgcgta ggccgagacc tcgtagcgct cgaggcccgc
     2881 gccggcggtg cgcgccgtga tcaggtccag catgtccgag gcgaggtcgt cgtccggcag
     2941 gttcgcgggc gggcggcggc cgaagacggt gttcggctcc atcgtcaggt ggtagaccga
     3001 caggtgcggc ggcgcgagcg cgagcgcctg cgcgaggtcg gcttccaggc ccgcgagcga
     3061 ctgacccggc agcgcgtaca tcaggtcgag gttgaaggtg ccgaagctgc gcgcggcctc
     3121 ctcggccgcc gccagcgcct gcgcgcggtc gtgcacgcgc ccgagcgccg cgagctgcgc
     3181 gtcgtcgaag ctctgcacgc cgatggacag gcgcgtcacg cccgcctgcg cgaaggcgcg
     3241 gaagcgatcc ttctcgaacg tcccgggatt ggcctccatc gtgatctcgc agtcgccggc
     3301 cagcggcagc agcatgcgca ggtcggagac gaggcgctcg atgcccgccg gggagaacag
     3361 gctgggcgtg ccgccgccga tgaacacgct gtgcacctgc cggccccaga cgagcggcag
     3421 cgcacgctcg aggtcggcgc gcagcgcgtc gaggtaggcg gtctcgggca ggtcggcggg
     3481 cgtcgcgccg gcggtccgga attcgtgcga gttgaagtcg cagtacgggc acttgcgcag
     3541 gcaccacggc aggtgcacgt agaccgccag cggcggcggc gcgggcagcg cgagcgtccc
     3601 ggggcggtgg taccactgcg gatcgcgctc gggcggcccg gcggcatcgc cctcgcccgg
     3661 tgccggatcg atggggatga cgttcatcgc ccctgcagcc gccaggcggc gcgcatcagc
     3721 tcgcgcatgc gcgcggccgc gagcgcgcga tggctgtgcg cgttcttcac ttccggggcc
     3781 agctgcgcga ccgtcgcgcc cagggccggg atgtacatca gcgggtcgta gccgaagccg
     3841 ccctggccct cgagcgccga caggatctcg ccctcccagc gcccgaccgc caccagcggc
     3901 tgcgggtcgt ccgccgcccg gatcgccacc agcgtgcaca cgaaggccgc atggcgatgc
     3961 tcggccgccg ccatgcgctc cagcagcagc gcgttgttgg cagcgtcctg cgtctcgcgc
     4021 acgacctcgc gcggcagcgc ccgccccgcg ctggccgcga ccacggcgta gttggccgag
     4081 accacgcccg gcgcaccgcc gagcgcgtcg acgcacaggc cggagtcgtc cgcgatggcc
     4141 ggcaggccgg tcgcggccga ggcgtggcgc gccttggcga gcgcgttctc gatgaacgtg
     4201 acgtgcggct cctcggcctc ggacacgccc agctcgccct ggcgcacgag cgcgacgccc
     4261 aggccgtcga acagcgcctg cagctcggca agcttcttcg cgttgttgga ggcgaggacg
     4321 agcttcatgc ggataccccg tggcgtacgg ttcaggcggc gaggccgagc gcggacttct
     4381 gcgccgcgat cagctggcgg atgccgccgt cggcgaggtc gagcatctgg ttcatctcga
     4441 tgcgcgagaa cggcgcgccc tcggcggtac cctggatctc gatgaagtgg ccggcaccgg
     4501 tcatcaccac gttcatgtcc gtgtcgcagg ccgagtcctc gacgtactcg aggtccagca
     4561 gcggcgtgcc ctcgacgatg cccaccgaca ccgcggcgac gaagcccgtc aggggcgtgc
     4621 gctcgatctt gccggccgcc agcagcgtcg cgaccgcgtc gtgcgccgcg acgcaggcgc
     4681 cggtgatggc cgcggtgcgc gtgccgccgt cggcctgcag cacgtcgcag tcgagcgtga
     4741 tggtgcgctc gccgagcagc ttcaggtcga acacggcgcg cagcgagcgt ccgatcaggc
     4801 gctggatctc ctgcgtgcgc ccgctctgct tgcccttggc ggcctcgcgg tcgctgcggg
     4861 tgtgcgtcgc gcgcggcagc atgccgtact ccgcggtcac ccagccctcg cccgagccct
     4921 tgcggaacgg cggcacgcgc tcctcgacgg aggcggtgca cagcacgcgc gtgccgccga
     4981 actccacgag cacggagccc tcggcgtgcg cggtgaagcg gcgcgtcagg cggacggggc
     5041 ggagctcgtt ggcggcgcgg gcgccggaac gctggaaggc catggatgcg gttcctcgaa
     5101 atggaaacgc cgggctgggt gcccggcgcc gtggattgtc gtcgatgcgg cgatgccgcc
     5161 cgcgcgcgtc aggtcttcct gggaccgctg cgctggatgg cctcgttgat ctcgcggatc
     5221 gagcgctcga tctcgtcgtc gtcgatgtca ggcaggaacg aatcgccggt catgctggcc
     5281 tggatcgtcg aggcgaagac gtcttcgccc agcgagcgcg tcgacacgcc gccactgcgg
     5341 tcctcgtcct ccgagccttc ccactcgacc gccagcgcag tgacgttgtc gctcttctcg
     5401 ccggcattgc gcaacgcctg ctcgacgagc tcgggcaccg agtcgtgcag cgggtaggtc
     5461 gccatcaggt tgacgatgtc ggtgtcctcg acgctgctcc acaggccgtc cgagcacagc
     5521 agcacgcggt cgccggcgcg cagcaccatc gggccggcgc tgtcgatcac cggcttgccg
     5581 gggctgccca ggcaggtgaa caggacgttg cggttgacct tctcctcgat cggcacgacg
     5641 tgcgagagcg cctcgcgcag ctcggagtac gagtggtcgc gggtgcgggc gatcaggcgg
     5701 tcgccgcgca tcaggtacag gcgcgagtcg ccgcagtggg cccagtagat cgagccgccc
     5761 tgcagcacgc aggcgacgac ggtggtgcgg ggcgtgtcgg tgaggccgcg gtcggtggca
     5821 tagcgcagca gctggtggtg gccggcgagg atggcgtcct ggaggaagcg ctggggatcc
     5881 ttcaggtcgg gcttggcctc cttctggaag taggccgcga gcgtctgcag cgcgagctgc
     5941 gaggcgactt cgccctcggg atggccaccc atcccgtcgg ccagcgcgaa cagccccgag
     6001 tcccgcgtgt agcaatagcc catgcggtcc tcgttcttct cgcggccgcc cttgcggctg
     6061 acttggtacg ttccgaaccg catcggtcac tcctcgtgtc gggcccaacg cacgcggcaa
     6121 gcggcggccc tggcggattg ggtgggggcc gcgcccggac gccgcgcggc ggtcacgggt
     6181 gggccccgga tttcaggttc tcgatctgca gcttcaggcg ctcggagaag ctcagcgtcg
     6241 tgtagcggcg ctcgatctcg cgcgaaagct ccttctgcag cgcgaacacg ctctgcggcc
     6301 gcgagagcgg gtcgagcgcc atgcaccact cgatcacctc gatcaggttg tccgagtaga
     6361 cgttgcgcag gcgcgagagc gacagcggca gccggtcctt gtcgatgcgc tggggcgcgt
     6421 cgttgggcgg atagccctgc atgcacgcgt acaggcaggc gccgatggcg tagatgtccg
     6481 tccacggccc cagcgagccg tcgcggcggt acatctccgg cgcggcgaag cccggcgtgt
     6541 acatcgggcg gatgaactgg ccttccttgc tcagcacctc gcgcgcggcg ccgaagtcga
     6601 gcagcacggc cttgttgtcg ttggtgatga agatgttggc cggcttgatg tccaggtgca
     6661 gcatcttgtg ctggtgcacg atgcgcaggc cgcgcagtat ctcgtcgaac agcgagcgga
     6721 tcgtcgattc gcgcagcacc ttttcgcgct tgaggtcgcg cgcggtcacg atgaaatcct
     6781 gcagcgtgtc gccctgcagg taattcatga ccatgtagac ggtttcgttc tcgcggaaga
     6841 aattcaacac cgagaccacg ctcgcatggg aaatctgcgc gagcgaccgg ccttcctcga
     6901 agaaactctt gaggccgagg cgatagagcg gctgcttctc cggcttgacg cgcgggatca
     6961 gctcgcctgg cgagcgatcg gcgagagatg ccggcagata ttccttgagg gcgacgagat
     7021 ggcgcgtctc gtcttcggcc aggtagacga tgccgaaacc gcctgcagcg atcttcttga
     7081 cgatctgata gcccccgacg acggtacccg agggtagcgg cgcgggtttt ggctttgaca
     7141 taatctgatt ttgcaacctg gcaccagaat catggcagtt tacagcatga ccggcttcgg
     7201 aaacgcctcg gcgacggcgg cttcggccgc ctccaccgag ggcgcagcgc ccgccgcccc
     7261 cgccgattcc gcggccgtca ccgtcgaact gcgatcggtc aacagccgct tcctcgacct
     7321 cgccttccgc atgcccgacg agctgcgcca gcacgagggc gcgctgcgcg aactcgtctc
     7381 ggcggcctgc cggcgcggca aggtcgacat ccgcgtcagc gccgcgcgca cgagcggcga
     7441 cgactggccg cagcccacgc ccgaccagct caaccgattg tcccgcctgg aaggcgccgt
     7501 gaccgcctgg atgccgaagg cggcgccgct gagcgtgaac gaagtcctgc attggtgccg
     7561 ttcggcggcg cccgcgcccg cggcgcgcat ggacgaggcg ctgctggatg ccgcgcgccg
     7621 ggccgtcgcg gccctgaagg aagcccgcgc gcgcgagggc gacaagctcg tcgcgatcct
     7681 gcgcgagcgc atcgtgcgcc tgcgcgagct cgcggcgcag gccgagccgc tggtgccggc
     7741 ggtcgtcgag cgccagcagc agcgcttcct cgagcgctgg aacgacgcac tgaagtccac
     7801 tggcagcgcc gacgtcccca ccgacgccct gcgcgagcgc gccctcaacg aggcggcgac
     7861 cttcgcgctg cgcatcgacg tcgccgagga gctgtcgcgc ctgcgcgccc acctcgagga
     7921 gatcgaccgc ctgctctcgg ccggcggcga gctcggcaag cggctggact tcctgatcca
     7981 ggaactgcac cgcgaggcca acaccctcgg ctccaagtcg gccgcgctgg agttgaccgg
     8041 cgtctcggtg gagatgaagg tgctgatcga gcagatgcgc gagcaggtgc agaacgtcga
     8101 gtgaagcggg cgataccggc cgcggtatcg caccatgcgt cccgcggtgc cggcgggcgg
     8161 caccccgacg gccgcgattg acgacgacgg gcgggtgcga cgatcctgcg ccccgtcgtc
     8221 cacaacgcac cgctccatgc cccgctccga catccgtttt cgccgcaccg gcactctcgc
     8281 cgccgtcgta ctgctgaccg tcggcgccgc gcacggccag gccaaggatc acgccgtcga
     8341 cgtatggcct gccgcgccgg cgcctgccat cgcgcccgag tccatcctcg cccaccagga
     8401 cgtcgctgcg ctcggccgcc agttgcaggt cggcgacatc gtcttcacgc gcgtcggcgc
     8461 ctatccgttc cgcaaggtgg ccgacgcgac gggcacctgg acgaaccacg tcgggatcgt
     8521 gctgtccacc gccggccccg agccggtgct cggcgaaagc cggttcccgt tctcgggcac
     8581 gacccccttc gcgcgcttcg tggcgcgctc g
//
LOCUS       DASVLN010000193         9867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_63087, whole genome shotgun sequence.
ACCESSION   DASVLN010000193 DASVLN010000000
VERSION     DASVLN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63087"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..2038)
                     /locus_tag="VF453_04420"
     CDS             complement(5..2038)
                     /locus_tag="VF453_04420"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7686927.1"
                     /translation="MILNCSRSALSAVSVAVLAMLSACGGSSSGPSPQATADSASVPW
                     NAATALDVTSNDAAASGAALAVQSQPAHGTASVQGGKIVYTPTAGYFGADSFSYLLGN
                     SSAQVNVTVLASMTIQGTVHDDAMPGAQVVLSVDGVAQPAVTADADGNYTLTVTTANP
                     NAFLSLQATGVGAQSNVALSSLVGDAASTAAAADSQGAVSAQALPAANVTNVSTAVAV
                     LSQQALGKAPASTADLLAAQGQFTAAQAVQMAAAIKLVADSGVALPSGSANTLALVSN
                     PAAYTAFVTDQATHNATAFTATQNAVLADPSLAVAPPAPATGSGDVTLLVTLGQGAGA
                     QGATRLTMKADGTATIGGSVPLTAHWTTDGGQITVTYDAPYVYENNATASDGRQYLVN
                     ESDTGFKLRQLSGPPGAGPATLDTLGSITWEGGPSNGQTATLADNWVAETMVGQPQAI
                     VAADVAVGTEWAGFLTTDFDPSTSNWTDQDVLKIVDATHVLFERTNVTGTWALNNGAL
                     EVTTPAGLFRYTRLFTGPKGEERWLDEHIVDGTAAWSFDAATVKVTPGLAFTAASAAH
                     DWRSYVNAGLASSSFYIDLAADGTGVPVLDDASGNPIGSPYWPGTWTIAPDGSEVFTR
                     SYCNDQTLSCGQGEVRSWTLLATAGSNIYVMERLQELGSMDQYRINVYTDADH"
     gene            2444..2956
                     /locus_tag="VF453_04425"
     CDS             2444..2956
                     /locus_tag="VF453_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7686928.1"
                     /translation="MTIELILEAGGEPSRDAIAERAGVGIGTLYRHFPDQQALLHAVA
                     RHALGKSIEAGEAIVADAGDGIEALRRYMHAALDNGIGVVNMIHPLLENHDWPDLLAR
                     AGAMMKAIVTRAVRDGRLGDDVSERDIVFAMIRFARPLAIGLPQVEERAIAHRHLNFY
                     IDGLCRRHDR"
     gene            3076..3471
                     /gene="arr"
                     /locus_tag="VF453_04430"
     CDS             3076..3471
                     /gene="arr"
                     /locus_tag="VF453_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HEX7686929.1"
                     /translation="MQLLDSGPFFHGTKADLQVGDLLTAGFTSNYREGLVMNHVYFTA
                     LSKGAGLAAEMAAGSGRPRVYVVEPTGQFEHDPNVTDKKFPGNPTRSYRSRHPLKIIG
                     EIERWERFDADFVVGLRRKVREGMGEIIN"
     gene            complement(3520..5157)
                     /locus_tag="VF453_04435"
     CDS             complement(3520..5157)
                     /locus_tag="VF453_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7686930.1"
                     /translation="MAGQDTWWRRGVIYQVYPRSFQDTDGDGIGDLRGIRRRLPHLAD
                     LGVDAAWISPIYPSPMADFGYDVADYCGIDPRFGTLADFDALVADAHARGLRVILDFV
                     PNHSSSQHPWFLESRSSRANPRRDWYLWRDPAPDGGPPNNWLSNFGGPAWTFDEATGQ
                     YYAHAFLPQQPDLNWRNPEVRAAMYDALRFWLLRGVDGFRVDVIYHVIKDAQFRDNPP
                     NPDYVAGRDPQSHRQLPQYTVDQPEVQQVVAEMRGVLDAFSTPGRERVLIGEIYLPIS
                     RLVAYYGADRDGLLQGAQLPFNFHLIGTAWQADAIDRVVRQYEAALPTGAQPNWVLGN
                     HDKPRIASRVGLAGARLAAMLLLTLRGTPTLYYGDEIGMTDVTIPPDEVQDPFEKNEP
                     GKGLGRDPQRTPLPWDGGPNAGFTTGRPWLRLGADADVRNVQAQAADPGSMLSLYRRL
                     LAWRRERPALNAGDWTPLGVHGTVLAYARIHEGGDRVLVLLNFGDGQAPVPAELRAEV
                     TAAATWLSTSGDRASLPWPAVLQAHEGLMIGARASGQ"
     gene            5222..6886
                     /locus_tag="VF453_04440"
     CDS             5222..6886
                     /locus_tag="VF453_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX7686931.1"
                     /translation="MINDLWYKNAIVYCLSVDSYMDANGDGCGDFEGLARRLDYLHGL
                     GITTLWLMPFQTSPRRDSGYDVADYYNVDPRFGTLGDFVEFTHGAKQRGMRVLIDLVV
                     NHTSDQHPWFQQARADPASRYRDWYVWAEEPPPDATDGVVFPGVQKSTWTYDKKARLW
                     YFHRFYDFQPDLDTSNPHVQAEILKIMGFWTQLGVSGFRMDAVPFVISLKGADVRRRK
                     PDFDMLRSFRELLQWRQGDAVILGEANVLPKDDLAYFGDEGERLHMMFNFQVNQGLFY
                     ALAAADGRPLMKALAATKPRPATSQWASFLRNHDELDLGRLTPAQRERVFAEFAPDPT
                     MQLYGRGIRRRLAPMLGSDRRRIELSYSLMLTLPGTPVLRYGDEIGMGDDLGLPEREC
                     ARTAMQWSDEPHGGFTCAKRPAVAVIAEGAYGFPHVNVAAQRRDPESLLNWMERVIRM
                     RKEVPEIGWGDYEVLDAGDPAVVMLRYEWRNNAVLFIHNLDNKPCEVRFPAQDSHGRR
                     GVLVNLLSEDHSRAGDDGEHRVLMEPYGYRWFRVGGLDYLLERSDV"
     gene            complement(6903..7319)
                     /locus_tag="VF453_04445"
     CDS             complement(6903..7319)
                     /locus_tag="VF453_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7686932.1"
                     /translation="MTEHLGRLVDHVHLRVRDLAASRRFYAAALEAIDRLDLLADGDG
                     CFFADELYVDVADGPVSHVHLAFQARERSQVDRFHAAALAAGGVDNGGPGLRAYHAGY
                     YAAFVLDPDGNNIEVVHHGEVERSADSVTVDRREDS"
     gene            complement(7316..>9867)
                     /locus_tag="VF453_04450"
     CDS             complement(7316..>9867)
                     /locus_tag="VF453_04450"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic beta 1-2 glucan synthetase"
                     /protein_id="HEX7686933.1"
                     /translation="DKRLAPFVPTRQVRPESALAPRLPTGDLALFNGYGGYAKDGREY
                     VVAPPAGVRTPAPWANVIANPDFGTVVSEAGAAYTWAENAHEWRLTPWHNDPVSDPSG
                     ETFHVRDEETGAFWMPCGTVSSGDGAPGRAAIARHGFGYSTFEQSAHGIHTELRVFCA
                     IDAPVKFSVLTLRNDSGRPRKVSATGYVEWVLGALRPATAPHIVTDHREDGPATARNP
                     YSGDHADYTGFFDVDAEYQQAGSMTCDRAEFIGRNGSLLEPVCLKREGLSGRFGAAMD
                     PCAAFQVPVTLLEGESRVVVFRLGMGKTPADAERIALQFRGEAVAQAEFERVQAHWGK
                     TLGAVTVRSPDAALNALANGWLVYQTIACRLWARSGYYQSGGAFGFRDQLQDAMALVH
                     TRPELLRAQLLLAASRQFEEGDVQHWWHPPQGRGVRTHISDDYLWLPMALCRYVQATG
                     DVAVLRETVPFLEGRPLKPEDESYFDLPAVSHRSATLYEHAALAVKHGLRTGEHGLPL
                     IGGGDWNDGMNLIGEHGKGESVWMGFFVCEVLREFAGLARVHHDEVFARTCEDARQRI
                     AQALDEHAWDGAWYRRAYFDDGTPLGTAGGIECQIDSIAQSWAVLSGIAPRERARQAL
                     DSLGKRLVNPQQRIVKLLDPPFDGKGPNPGYIAGYVPGVRENGGQYTHGAIWAAMAFA
                     AAGDVRRAWDVVDMINPVNHARTPAEVAVYKVEPYVMTADVYSVAPHTGRGGWSWYTG
                     SAGWMYRLLVEAVLGLRREQAEGGPTLVLDPRVPPHWTAFEVDYVHGETFYRLQFARA
                     ADAGAPRVVLDGRALEGNRLLLVDDGRQHSVQVVLPATAQVPAAQSAELLL"
BASE COUNT         1426 a         3386 c         3562 g         1493 t
ORIGIN      
        1 cggatcagtg gtcggcgtcg gtgtagacgt tgatccggta ctggtccatg ctgcccagtt
       61 cctgcaggcg ttccatcacg tagatgttcg agccggcggt cgccagcagc gtccacgaac
      121 gcacctcgcc ctggccgcac gacagcgtct gatcgttgca gtacgagcgc gtgaacacct
      181 ccgagccgtc gggggcgatc gtccaggtgc cgggccagta cggcgaaccg atcgggttgc
      241 cgctggcgtc gtcgagcacc ggcacgccgg tgccgtccgc ggccaggtcg atgtagaacg
      301 acgagctggc caggccggcg ttgacgtagc tgcgccagtc gtgcgcggcg ctggccgccg
      361 tgaacgcgag gcccggcgtc accttgacgg tcgccgcgtc gaacgaccac gccgcggtgc
      421 cgtcgacgat gtgctcgtcg agccagcgct cctcgccctt cgggccggtg aacaggcgcg
      481 tgtagcggaa caggcccgcc ggtgtggtca cttccagcgc gccgttgttc agggcccagg
      541 tgccggtgac gttggtgcgc tcgaacagca cgtgcgtcgc gtcgacgatc ttgagcacgt
      601 cctggtcggt ccagttcgag gtggacggat cgaagtccgt ggtgaggaag ccggcccact
      661 ccgtgcccac ggccacgtcg gcggcgacga tggcctgcgg ctggcccacc atcgtctcgg
      721 cgacccagtt gtcggcgagc gtggccgtct gcccgttgga cgggccgcct tcccatgtga
      781 tgctgccgag ggtgtccagc gtggcgggcc ccgcgcccgg cgggccgctg agctggcgca
      841 gcttgaagcc ggtgtcggac tcgttgacga ggtactggcg gccgtcgctc gcggtcgcgt
      901 tgttctcgta cacgtagggg gcgtcgtagg tgaccgtgat ctggccgccg tccgtggtcc
      961 agtgggccgt cagcggcacg ctgccgccga tcgtggcggt accgtccgcc ttcatggtca
     1021 ggcgcgtcgc gccttgggcg ccggcgccct ggccgagcgt caccagcagc gtgacgtcgc
     1081 cggagcccgt ggccggcgcc ggcggcgcga ccgcgaggct cgggtcggcc agcacggcgt
     1141 tctgcgtcgc cgtgaaggcg gtcgcgttgt gggtggcctg gtcggtcacg aacgcggtgt
     1201 aggccgccgg gttcgacacc agcgccagcg tgttcgcgct gccgctcggc agggccacgc
     1261 ccgagtcggc gacgagcttg atcgcggcgg ccatctgcac cgcctgcgcc gccgtgaact
     1321 ggccctgcgc ggccagcagg tcggccgtgc tggcgggcgc cttgcccagc gcctgctgcg
     1381 agagcaccgc caccgcggtc gacacgttcg tgacgttggc ggccggcagc gcctgcgcgc
     1441 tcaccgcgcc ctggctgtcc gccgcggcgg ccgtcgaggc cgcgtcgccg accaggctgg
     1501 acagggcgac gttcgactgc gccccgacgc cggtcgcctg cagcgacagg aacgcgttgg
     1561 gattggccgt cgtgaccgtc agggtgtaat tgccgtccgc gtcggcggtc acggcgggct
     1621 gggccacgcc gtccaccgac agcacgacct gggcgcccgg catcgcgtcg tcgtgcaccg
     1681 tgccctggat cgtcatcgac gccagcacgg tcacgttcac ctgcgcgctg ctgttgccga
     1741 gaagatagga gaacgaatcc gcgccgaaat acccggccgt cggcgtgtag acgatcttgc
     1801 cgccctgtac ggaggcggtg ccgtgcgccg gttgcgactg caccgccagc gcggcgcccg
     1861 atgccgccgc gtcgttggac gtcacgtcca gcgcggtcgc cgcgttccag ggcaccgacg
     1921 ccgagtccgc ggtcgcctgc ggcgagggac cggaagagga gccgccgcag gcgctcagca
     1981 tcgcgagcac ggcgaccgac accgccgaca gcgcacttct cgaacaattc aggatcatcg
     2041 tggttccctt gcccgtgtgt gaggaagagt cggagcgtct gggctccagc cccgatcacg
     2101 gtttcggcga ggggaggggg tccttgacgc gtggactttg gaagagggcc gatgtgtgcc
     2161 gaagtcgaca cattgcgtgg ctccgtcggg ggcgggcggg caggcctccg agcggataca
     2221 ggctcgcatg cctgaagctt gacggcctta aacggaggaa tatgctccga ttatgtttga
     2281 cgccgatgcg ctcccattgg cgcggcgccc catgaaatgg tggcacggcc gtggccgcgt
     2341 ccgctgccac gacgctccct cttcttcgtc tgcattcgga agaggaggaa caggaatccg
     2401 tatgcgcgct gatgcacaaa gaaaccgcca acgccttctg gacgtgacga tcgaactgat
     2461 cctggaggcc gggggagagc cttcgcgcga tgccatcgcc gaacgcgccg gtgtcggcat
     2521 cgggacgctt taccgccact tccccgatca gcaggcgctg ctgcacgccg tcgctcgtca
     2581 cgccttgggc aagagcatcg aggcgggcga agcgattgtt gccgacgctg gagatggcat
     2641 cgaggcgcta cgccgctata tgcacgccgc gctcgacaac ggcatcggtg tggtcaacat
     2701 gattcacccg ctgctcgaga accacgactg gcccgatctc ctggcgcgcg ccggggcaat
     2761 gatgaaggcc atcgtcaccc gcgctgtccg ggacgggagg ctgggcgacg acgtctcgga
     2821 gcgggacatc gtcttcgcga tgatccgctt cgcgcgaccg ctggccatcg ggttgccgca
     2881 ggtcgaggag cgagcgatcg cgcacaggca cctgaatttc tacatcgacg gcctttgccg
     2941 gcgacacgac cgctgatccg cgagcgatgg ctcatccgga tgcggtgatg gcgacggaag
     3001 caggcagctc ggtctgaacg tccgggcgat ttctccagct cgcatccgcg acgcgtcgat
     3061 ctccaaaggg cattcatgca actcctcgat tcgggaccct tctttcacgg cacgaaggcg
     3121 gaccttcaag ttggcgatct cctgaccgcc gggttcacat cgaactacag ggagggtctg
     3181 gtcatgaacc acgtctattt caccgctctg agcaagggag ccggcctggc tgcggagatg
     3241 gccgccggca gcgggaggcc acgtgtttac gtcgtcgaac ccaccggcca gttcgagcac
     3301 gaccccaacg tgacggacaa gaagtttcca ggcaacccga cgcgttccta tcgaagccga
     3361 catccgttga agatcattgg cgagatcgag agatgggagc ggttcgacgc cgacttcgtc
     3421 gtgggactga gacgaaaggt gcgagaaggg atgggcgaga tcatcaactg accacgaatc
     3481 acgatcgtcc ctgatgaacg ggtgaacgcc ggatggactt cactgtcccg aggctcgggc
     3541 gccgatcatg agcccctcgt gcgcctgcag cactgccggc cacggcaggg acgcccggtc
     3601 gcctgaggtg gagagccagg tcgcggccgc cgtcacctcc gcccgcagct ccgccggcac
     3661 cggcgcctgg ccgtccccga aattcagcag caccagcacc cggtcgcccc cttcatggat
     3721 gcgtgcgtag gccagcaccg tgccgtgcac gcccagcggc gtccagtcgc ctgcattgag
     3781 cgccggccgc tcccggcgcc acgccagcag gcggcgatac agcgagagca tcgagcccgg
     3841 atccgccgcc tgggcctgca cgttgcgcac atcggcatcg gcgcccaggc gcagccaggg
     3901 ccggccggtg gtgaagccgg cgttcgggcc gccgtcccag ggcaggggcg tgcgctgcgg
     3961 gtcgcggccc aggcccttgc cgggctcgtt cttctcgaac ggatcctgca cttcgtcggg
     4021 agggatggtc acgtcggtca tcccgatctc gtccccgtag tacagggtcg gggtaccgcg
     4081 cagcgtgagc aggagcatcg ccgcaagccg tgcgccggca aggcccaccc ggctggcgat
     4141 gcggggcttg tcgtggttgc ccagcaccca gttgggctgc gcgcccgtcg gcagggccgc
     4201 ctcgtactgg cgcaccacgc ggtcgatcgc gtcggcctgc caggcggtgc cgatcaggtg
     4261 gaagttgaag ggcagttgcg cgccctgcag caggccgtcg cggtcggcgc cgtagtaggc
     4321 gacgagacgc gagatgggca ggtagatctc gccgatcagc acgcgctcgc gccccggggt
     4381 gctgaacgcg tccaacacgc cgcgcatctc ggccaccacc tgctggactt ccggctggtc
     4441 gacggtgtat tgcggcagct ggcggtggct ctgcgggtcg cgcccggcca cgtagtcggg
     4501 gttcggcggg ttgtcgcgga actgggcgtc cttgatcacg tggtagatca cgtcgacgcg
     4561 aaagccgtcg acgccgcgca gcaaccagaa tcgcagcgcg tcgtacatcg cggcgcggac
     4621 ctcgggattg cgccagttga ggtccggctg ctggggcagg aaggcgtggg cgtagtactg
     4681 gcccgtcgct tcgtcgaagg tccatgccgg accgccgaag ttgctgagcc agttgttggg
     4741 cgggccgccg tcgggagcgg ggtcgcgcca caggtaccag tcgcggcgcg gattggcgcg
     4801 gctgctgcgg ctctcgagga accacggatg ctggctcgag ctgtggttgg ggacgaagtc
     4861 gaggatcacg cgcaggcccc gcgcgtgggc gtcggcgacg agcgcgtcga agtcggcgag
     4921 cgttccgaag cgcgggtcga tcccgcagta gtcggccacg tcgtagccga agtcggccat
     4981 gggggaaggg tagatgggcg agatccacgc ggcgtccacc ccgaggtccg cgagatgcgg
     5041 caggcgccgc cggatgccgc ggaggtctcc gatgccgtcg ccgtcggtgt cctggaacga
     5101 gcgcgggtag acctggtaga tcaccccgcg ccgccaccac gtgtcctggc ctgccatccc
     5161 gctcctttca tcgccgtcgt gtcagggctc tcgggtgcgg caattgccgc gccctgcggg
     5221 catgatcaac gacctctggt acaagaacgc gatcgtgtac tgcctgtcgg tggacagcta
     5281 catggacgcg aacggcgacg gctgcggcga cttcgagggc ctcgcgcgcc ggctcgacta
     5341 cctgcacggg ctgggcatca ccaccctctg gctgatgccg ttccagacct ccccccgccg
     5401 cgacagcggc tacgacgtcg ccgactacta caacgtcgac ccgcgcttcg gcacgctggg
     5461 cgacttcgtg gagttcacgc acggcgccaa gcagcgcggc atgcgcgtgc tgatcgacct
     5521 ggtggtcaac cacacgtccg accagcatcc gtggttccag caggcgcgcg ccgatccggc
     5581 gtcccgctac cgtgactggt acgtgtgggc cgaggagccg ccgcccgacg ccacggacgg
     5641 cgtggtgttc ccgggcgttc agaagtcgac ctggacctac gacaagaagg cccggctctg
     5701 gtacttccat cgcttctacg atttccagcc cgacctcgac acgtcgaacc cgcacgtgca
     5761 ggcggaaatc ctgaagatca tgggcttctg gacgcagctg ggggtgtccg gctttcgcat
     5821 ggacgcggtg ccgttcgtga tctcgctcaa gggcgccgac gtgcgcaggc gcaagcccga
     5881 cttcgacatg ctgcgcagct tccgcgaact gctgcaatgg cgccagggcg acgccgtcat
     5941 ccttggcgag gcgaacgtgc tgcccaagga cgacctggcc tacttcggcg acgaaggcga
     6001 gcggctgcac atgatgttca acttccaggt caaccagggc ctgttctatg cgctggcggc
     6061 ggcggacggc cggccgctga tgaaggcgct ggccgcgacc aagccgcgcc cggccacgtc
     6121 gcagtgggcc agcttcctgc gcaatcacga cgaactcgac ctcggtcgcc tcacgccggc
     6181 ccagcgtgag cgggtattcg ccgagttcgc gcccgatccg acgatgcagt tgtacggccg
     6241 cggcatccgg cgccgcctgg cgcccatgct gggcagcgac cgccgccgga tcgagctgtc
     6301 ctacagcctg atgctgacgc tgccgggcac gcccgtgctg cgctacggcg acgagatcgg
     6361 catgggcgac gacctgggcc tgcccgagcg cgaatgcgcc cggacggcga tgcaatggtc
     6421 ggacgaaccg cacggcgggt tcacctgcgc gaagcggccc gccgtggcgg tcatcgccga
     6481 aggggcctac ggcttcccgc acgtgaacgt tgccgcgcaa cggcgggatc cggagtcgct
     6541 gctcaactgg atggagcgcg tgatccgcat gcgcaaggag gtgccggaga tcggctgggg
     6601 cgactacgag gtgctcgacg ccggcgaccc cgcggtcgtg atgctgcgct acgagtggcg
     6661 caacaacgcg gtgctgttca tccacaacct cgacaacaag ccgtgcgagg tgcgcttccc
     6721 ggcgcaggac tcgcacggcc gccgcggcgt gctcgtcaac ctgctgtccg aggaccacag
     6781 ccgcgcgggc gacgatggcg agcaccgcgt gctgatggag ccctacggct accgctggtt
     6841 ccgggtgggc ggcctcgact acctgctcga gcgcagcgac gtgtgatcgc cggccggcgt
     6901 cctcacgagt cttcgcgccg gtcgacggtg acgctgtcgg cgctgcgctc gacctcgccg
     6961 tggtgcacga cctcgatgtt gttgccgtcc gggtcgagca cgaacgcggc gtagtagccg
     7021 gcgtgataag cgcgcaggcc ggggccgccg ttgtcgacgc cgccggcggc gagggcggcg
     7081 gcgtggaagc ggtcgacctg cgagcgctcg cgcgcctgga aggccaggtg cacgtgcgag
     7141 accgggccgt cggcgacgtc gacatagagc tcgtcggcga agaagcagcc gtcgccgtcg
     7201 gccaggagat ccaggcggtc gatcgcctcc agcgccgcgg cgtagaagcg gcggctggcg
     7261 gcgaggtcgc gcacgcgcag gtgcacgtgg tcgacgaggc ggcccaggtg ctcggtcaca
     7321 gcagcagctc cgccgactgc gccgccggaa cctgcgcggt cgcaggcagc acgacctgca
     7381 cgctgtgctg gcgcccgtcg tcgaccagca gcaggcggtt gccctcgagc gcgcggccgt
     7441 ccagcaccac gcgcggcgcg ccggcgtcgg cggcgcgggc gaactgcagg cggtagaagg
     7501 tctcgccgtg cacgtagtcg acctcgaagg ccgtccagtg cggcggcacg cgcgggtcga
     7561 gcaccagcgt cgggccgccc tcggcctgct cgcgccgcag gcccagcacg gcctcgacca
     7621 gcaggcggta catccagccc gccgagccgg tgtaccagct ccagccgccg cgcccggtgt
     7681 gcggcgcgac gctgtagacg tcggccgtca tcacgtaggg ctcgaccttg tagaccgcga
     7741 cctccgccgg cgtgcgcgcg tgattgaccg ggttgatcat gtcgaccacg tcccaggcgc
     7801 gccgcacgtc gcccgcggcg gcgaaggcca tcgccgccca gatcgcgccg tgggtgtact
     7861 ggccgccgtt ctcgcgcacg cccggcacgt acccggcgat gtatccgggg ttggggccct
     7921 tgccgtcgaa cggcggatcc agcagcttga cgatgcgctg ctgcgggttg acgaggcgct
     7981 tgccgagcga gtcgagcgcc tggcgcgcgc gctcgcgcgg cgcgatgccc gagagcaccg
     8041 cccagctctg cgcgatcgag tcgatctggc actcgatgcc gccggccgtg ccgagcggcg
     8101 tgccgtcgtc gaaatacgcc cggcggtacc aggcgccgtc ccaggcgtgc tcgtcgagcg
     8161 cctgcgcgat gcgctgccgg gcgtcctcgc aggtgcgcgc gaagacctcg tcgtggtgca
     8221 cgcgcgcgag gcccgcgaac tcgcgcagca cctcgcagac gaagaagccc atccagacgc
     8281 tctcgccctt gccgtgctcg ccgatcaggt tcatgccgtc gttccagtcg ccgccgccga
     8341 tcagcggcag gccgtgctcg cccgtgcgca gcccgtgctt gacggccagc gccgcgtgct
     8401 cgtagagcgt cgcgctgcgg tgcgacaccg cgggcaggtc gaagtaggac tcgtcctccg
     8461 gcttgagcgg ccggccctcg aggaagggca cggtctcgcg cagcaccgcc acgtcgccgg
     8521 tggcctggac gtagcggcac agcgccatcg gcagccacag gtagtcgtcg ctgatgtgcg
     8581 tgcgcacgcc gcggccctgc ggcggatgcc accagtgctg gacgtcgcct tcctcgaact
     8641 ggcggctggc ggccagcagc agctgggcgc gcagcagctc gggacgcgtg tgcaccaggg
     8701 ccatcgcgtc ctgcagctgg tcgcggaagc cgaacgcgcc gccggactgg tagtagccgc
     8761 tgcgcgccca cagccggcag gcgatcgtct ggtacacgag ccagccgttg gccagcgcat
     8821 tgagcgcggc gtcgggcgag cgcaccgtga ccgcgccgag cgtcttgccc cagtgcgcct
     8881 gtacgcgctc gaattcggcc tgggcgaccg cctcgccccg gaactgcagc gcgatgcgct
     8941 cggcgtcggc gggcgtcttg cccatgccca ggcggaagac gaccacgcgc gactcgcctt
     9001 ccagcagcgt caccggcacc tggaacgcgg cgcacggatc catcgcagcc ccgaagcggc
     9061 cggacaggcc ctcgcgcttc aggcacaccg gctcgagcag gctgccgttg cggccgatga
     9121 actcggcgcg gtcgcaggtc atgctgccgg cctgctggta ctcggcgtcg acgtcgaaga
     9181 agccggtgta gtccgcgtgg tcgccgctgt aagggttgcg cgcggtggcg gggccgtcct
     9241 cgcggtggtc ggtgacgatg tgaggcgccg tggccggccg cagcgcgccc agcacccact
     9301 cgacgtagcc ggtggcgctg accttgcggg ggcggccgga gtcgttgcgc agggtcagca
     9361 ccgagaactt caccggcgcg tcgatggcgc aaaacacgcg cagctcggtg tggatgccgt
     9421 gcgcgctctg ctcgaacgtg ctgtagccga agccgtggcg cgcgatcgcg gcgcgcccgg
     9481 gcgcgccgtc gccgctggag acggtgccgc agggcatcca gaaggcgccg gtctcctcgt
     9541 cgcggacgtg gaaggtctcg ccgctcgggt cggagacggg gtcgttgtgc cagggcgtga
     9601 ggcgccactc gtgcgcgttc tcggcccacg tgtaggcggc gccggcctcg gacaccaccg
     9661 tgccgaagtc cgggttggcg atcacgttgg cccagggcgc gggcgtgcgc acgcccgcgg
     9721 gcggggcgac gacgtactcg cgcccgtcct tcgcgtagcc gccgtagccg ttgaacagcg
     9781 cgaggtcgcc ggtgggcagg cgcggcgcga gcgcggactc ggggcgcacc tggcgcgtcg
     9841 gcacgaaggg cgcgagccgc ttgtcgg
//
LOCUS       DASVLN010000194         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_224371, whole genome shotgun sequence.
ACCESSION   DASVLN010000194 DASVLN010000000
VERSION     DASVLN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224371"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VF453_04455"
     CDS             complement(<1..319)
                     /locus_tag="VF453_04455"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEX7686934.1"
                     /translation="MSAHPIPAHYRPIAGSERRPSPAAVRQGPADANEVLQVTITLRR
                     RPDGPPAPDFDAVGAVPLAQRRPLSRDEFAAKYGAHPDDIAKVVDFVRTAGMTVVETN
                     AARR"
     gene            567..1937
                     /locus_tag="VF453_04460"
     CDS             567..1937
                     /locus_tag="VF453_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX7686935.1"
                     /translation="MSLSLRSQRDPEPDPERVAAVRAAALPLAADESAFDDLLRRVGD
                     ARFVLLGEASHGTHEFYLVRERITRRLIARHGFDAVAIEGDWPDAWRVNRHVRGLGAD
                     ATPDDALAGFRRFPTWMWRNAVVTDFVGWLRGWNAALPPEHRCGFYGLDLYSLNASMA
                     AVLAWLDDAQPELAARVRERYACFEPFGGDTQAYGLMTGLGSTPSCERAVLAALDDLR
                     RRRAATRDTATADDAEAAFDAEQNAALVRDAERYYRTMYLSEVSSWNERDSHMASTLQ
                     ALEAHLSRPGRPARIVVWAHNSHLGDARATEMGAWRGEHNLGQLVRERHGDASFLLGF
                     TTHDGSVTAARDWDSDVQRKRVNPSRGDSIEGLLHAVGGARFWLPLRDDARLREALDA
                     PLLERAIGVIYRPDTERQSHYFEARVARQFDALVHVDRTRALEPLDRGPQWHDTEAPE
                     TYPQGL"
     gene            complement(1941..>2062)
                     /locus_tag="VF453_04465"
     CDS             complement(1941..>2062)
                     /locus_tag="VF453_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7686936.1"
                     /translation="CPNTAAYRRRLLARPSIARAVDEARPFRGWFPPGAPDRD"
BASE COUNT          251 a          753 c          794 g          264 t
ORIGIN      
        1 tgcggcgcgc ggcgttggtc tcgacgacgg tcatgccggc ggtccgcacg aagtcgacga
       61 ccttggcgat gtcgtcggga tgcgccccgt acttcgccgc gaactcgtcg cgcgagagcg
      121 gccggcgctg cgcgagcggc accgcgccga cggcgtcgaa gtccggcgcg ggcggaccgt
      181 ccggacggcg gcgcagcgtg atcgtgacct gcagcacctc gttggcgtcg gcggggccct
      241 ggcgcacggc ggcgggcgac ggacggcgtt cgctgcccgc gatggggcgg tagtgggcgg
      301 gaatgggatg ggcggacatc ggcggctctc ctgggcaggg tgcgcccggg cgacggcctg
      361 cggggccggc gtcggacgcc atggggttga tgaggcggcg gatcttgcgc gccgggccgc
      421 gggctcgccc atcccacgcg atggggggac tcgaggccaa gtcccctgtt tgaaggatgg
      481 ttggcgcggc cggccgggcc ggggcgattg cccgcagagg tgtcgggagc ccggcttgcc
      541 ggtccttcgt gcaccgggag cctgccatga gcctgtcgtt gcggtcgcag cgcgaccccg
      601 agcccgatcc cgagcgcgtc gccgccgtgc gcgccgccgc gctcccgctg gccgcggacg
      661 aatccgcgtt cgacgacctg ctgcgtcgcg tcggcgacgc gcgcttcgtg ctgctgggcg
      721 aggcctcgca cggcacgcac gagttctacc tcgtccgcga gcgcatcacg cgacgcctga
      781 tcgcgcgcca cggcttcgac gcggtcgcca tcgagggcga ctggcccgac gcgtggcgcg
      841 tgaaccgcca cgtgcgcggg ctgggcgccg acgccacgcc cgacgacgcg ctcgccggct
      901 tcaggcgctt tccgacctgg atgtggcgca acgcggtcgt cacggacttc gtcggctggc
      961 tgcgcggctg gaacgcggcg ctgccgcccg agcatcgttg cggcttctac ggcctcgacc
     1021 tctacagcct gaacgcgtcg atggccgcgg tgctcgcctg gctcgacgac gcgcagccgg
     1081 agctggccgc gcgcgtgcgg gagcgctacg cctgcttcga gcccttcggc ggcgacacgc
     1141 aggcatacgg cctgatgacg ggcctgggct cgacgccgtc ctgcgagcgg gccgtgctcg
     1201 ccgcgctcga cgacctgcgg cgccggcgcg ccgcgactcg cgacacggcg acggcggacg
     1261 acgccgaggc cgcgttcgat gccgagcaga acgccgcgct cgtgcgcgac gccgagcgct
     1321 actaccggac gatgtacctg agcgaggtgt cgtcctggaa cgagcgcgac tcgcacatgg
     1381 cgagcacgtt gcaggccctc gaggcgcacc tgtcgcgccc gggccggccg gcgcgcatcg
     1441 tcgtatgggc gcacaactcg cacctcggcg acgcgcgcgc caccgagatg ggcgcgtggc
     1501 gcggcgagca caacctcggc cagctcgtgc gcgagcgcca cggcgacgcg tccttcctgc
     1561 tcggcttcac gacccacgac ggcagcgtga cggcggcgcg cgactgggac tcggacgtgc
     1621 agcgcaagcg cgtcaacccg tcgcgcggcg actcgatcga gggactgctg cacgcggtgg
     1681 gcggcgcgcg cttctggctg ccgctgcgcg acgacgcgcg cctgcgcgag gccctggacg
     1741 cgccgctgct cgaacgtgcg atcggcgtga tctaccgccc cgacaccgag cgccagagcc
     1801 actacttcga ggcgcgcgtg gcgcgccagt tcgatgcgct cgtgcacgtc gaccgcacgc
     1861 gcgcgctcga gccgctcgat cgcggcccgc agtggcacga caccgaggcg ccggagacct
     1921 acccgcaggg cctgtgagcg tcagtcgcga tccggcgcgc ccggcgggaa ccagccgcgg
     1981 aacggccgcg cctcgtccac cgcgcgcgcg atcgagggcc gggccagcag ccggcggcga
     2041 taggcggcgg tgttcgggca gc
//
LOCUS       DASVLN010000195         5959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_224397, whole genome shotgun sequence.
ACCESSION   DASVLN010000195 DASVLN010000000
VERSION     DASVLN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224397"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="VF453_04470"
     CDS             complement(<1..1297)
                     /locus_tag="VF453_04470"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7686937.1"
                     /translation="MSFDHQAQPAHEQQDDLAAEAATPETNGFAALGLAPEILRGIAE
                     AGYDEPTEVQARAIPAAIAGGDLLVSSRTGSGKTAAFVLPALQRILEARRDPAKRPQR
                     GQPSGPRVLVLAPTRELAMQVSRACQTYGRGLQGLRVATVVGGVPYAAQLRALRGPLD
                     VLIATPGRLIDHIGAGKALMSQVELLVLDEADRMLDMGFIDDIHHVAAQLSPARQTVM
                     FSATFEGNVGHLAQELTREPQRIRVSAPTEQHTQIEQRLHWADNRAHRDALLDQLLTD
                     RDLDQCVVFTSTQRDADELADRLAQIGHSVAALHGGMPQGRRNRVLGALRMRQLRVLI
                     ATDVAARGIDVPTISHVINYGLPMKAEDYVHRIGRTGRAGRSGIAVTLAERRDVPMIR
                     RIERFTTHAIPPSVVAGLEPKLAPPPAGRSGGFRDDRGPG"
     gene            1855..3837
                     /locus_tag="VF453_04475"
     CDS             1855..3837
                     /locus_tag="VF453_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEX7686938.1"
                     /translation="MPDHKTALPTSHVLVVSADATWRDDVVAGLDAAAARLDNPFALR
                     AVAVAQASSAPERVLADGDVQIVLIDHGDHGPGAVATHQDVAELARGIIALRPELSLY
                     VTLEDDENKMLLEQLASNVVHGYFYRGERDYNGWARILAAEIAEKSATPFYDELRHYV
                     RMAKDSWHTPGHAGGDSFQGSPWVGDFHDFVGENMLRADLSVSVEMLDSLMHPTGVIA
                     QAQRLAAQAFGARKTYFATNGTSTANKVIFQTLLAPGDKLLLDRNCHKSVHHGVILSG
                     ARPVYLDSSVNRKYGLFGPVPRATVLEAIVAHPDARALILTSCTYDGLRYDLAPIVEA
                     AHAAGIKVIVDEAWYGHARFHPALRPTALESGADYCTQSTHKVLSAFSQASMIHVNDP
                     AFDEHLFRENFNMHASTSPQYNLIASLDVARKQAVMEGYRLLDRSLKYAQELRERINA
                     TGAFRALELADLLPEELAGDNIQLDPTKITIDISPSGFSSDELREELFERFNIQVEKS
                     THNTLTLLLTVGTTRSKVSRLFDALLRLSKERRPARANLRMPELPHFTRLACLPRDAF
                     YEYGERLPLLDEQGQPNAALEGRVCCDQITPYPPGIPVLVPGQVITAEIIGFLTRLMR
                     MQKSIEMHGLATCEGEECLRVLRPHELDALAQRSRL"
     gene            complement(3838..5139)
                     /locus_tag="VF453_04480"
     CDS             complement(3838..5139)
                     /locus_tag="VF453_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7686939.1"
                     /translation="MTSRNLDPAFWERARRHLVRYGGSFEPVIIERAAGSLLFDADGR
                     EILDFTSGQMSALLGHSHPDIVATVRASAGQLMHLNSTLLSRPVVDLAQRLAELAPGR
                     LERCLLLSTGAESNEAAIRLAKLVTGGHEIVGFSQSWHGMTGAAAAATYSAGRRGYGP
                     AMPGSMAIPAPNAYRPRFVRDGRHDWQAELDDGFDQVDRQSTGALAAFIAEPILSSGG
                     ILDLPEGYLAALKRKCEERGMLLIVDEAQTGVGRTGMMFACERDGVAPDILTLSKTLG
                     AGLPLAATITTAEIEERAHERGYLLYTTHVSDPLPAAVGLTVLDVVRRDGLVERARVA
                     GGRLRDGLLALQERHERVGDVRGRGLMLGLEVVQDRATKAPANDYGAAVARRCMALGL
                     SMNIVKLPGMGGVFRIAPPLTVTDAEIDRAVAILDEALATA"
     gene            5240..>5959
                     /locus_tag="VF453_04485"
     CDS             5240..>5959
                     /locus_tag="VF453_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7686940.1"
                     /translation="MPASIDIELARSFVAMADAGTLVGAAGRVGRTQSALSMQLKRLE
                     ETVGRTLFDRSPQGLRLTAAGDLLLGHATRLLRVHDEALAELSGRGLAGRLRLGCPED
                     YAVRLLPELLRRFGSQHPRVEFEVACAPTPRLRAMLARHAIDVALVSQPQEGEDAGAI
                     RVEPLVWVGRADVDPAAFDPLPLALSEPEAFDHQAPLEVLRRRGRAWRIAHASPHLLG
                     LLSMARSGLAITVLTGTAVPAD"
BASE COUNT          822 a         2113 c         2191 g          833 t
ORIGIN      
        1 cgccggggcc gcggtcgtcg cggaagccac ccgagcgacc ggccggcggc ggcgcgagct
       61 tgggttcgag gccggcgacg acggacggcg ggatcgcgtg cgtggtgaag cgctcgatgc
      121 ggcggatcat cggcacgtcg cggcgctcgg ccagcgtgac ggcgatgccc gagcggccgg
      181 cgcggccggt gcggccgatg cggtgcacgt agtcctcggc cttcatcggc aggccgtagt
      241 tgatgacgtg cgagatcgtc ggcacgtcga tgccgcgcgc ggcgacatcg gtggcgatca
      301 gcacgcgcag ctggcgcatg cgcagcgcgc cgaggacgcg gttgcgccgg ccctgcggca
      361 tgccgccgtg cagcgccgcg accgagtggc cgatctgcgc gaggcggtcg gcgagttcgt
      421 cggcgtcgcg ctgggtgctg gtgaagacga cgcactggtc gaggtcgcga tcggtcagca
      481 gctggtcgag cagggcgtcg cggtgggcgc ggttgtcggc ccagtgcagg cgctgctcga
      541 tctgcgtgtg ctgttcggtc ggcgcggaga cgcggatgcg ctgcggctcg cgcgtgagct
      601 cctgcgcgag atggccgacg ttgccctcga aggtggcgct gaacatcacc gtctggcgcg
      661 ccggcgacag ttgcgccgcc acgtggtgga tgtcgtcgat gaagcccatg tcgagcatgc
      721 gatcggcctc gtcgaggacg agcagctcga cctggctcat cagcgccttg ccggcgccga
      781 tgtggtcgat caggcggccc ggcgtggcga tcaggacgtc gagcgggccg cgcagcgcgc
      841 gcagctgcgc ggcgtagggc acgccgccca cgaccgtggc gacgcgcagg ccctgcaggc
      901 cgcggccgta ggtctggcag gcgcgcgaga cctgcatcgc gagctcgcgc gtgggcgcca
      961 gcaccagcac gcgcgggccg ctcggctggc cgcgctgcgg gcgcttggcc ggatcgcggc
     1021 gcgcctccag gatgcgctgc agcgccggga gcacgaaagc cgcggtcttg ccgctgccgg
     1081 tgcgcgagga cacgagcagg tcgccgccgg cgatcgccgc gggaatggcg cgcgcctgca
     1141 cctcggtggg ctcgtcgtag ccggcctcgg cgatgccgcg caggatctcg ggggcgaggc
     1201 cgagcgccgc gaagccgttg gtctcgggag tggccgcctc ggcggcgagg tcgtcttgtt
     1261 gttcgtgggc cggttgggcc tggtggtcga agctcatgca gtctttcaag cacggcgcgc
     1321 gccgccgcgc cccgggggag gccggagcat ggcgaacggg cgccacggcg ccgcctgccg
     1381 ggtcgcggac tcggcagcgc caatcgggtc gcgtagatga aaacggggca ggccgccgcg
     1441 ttgcagggcc gtggtcgcgg acgaccggcc tgtcgcggtg ggaatgaaag cccaggaaac
     1501 gtctggagcg acggcatcgc cgccgaccga acgccgtttc cgagtgccgt ccaccgggag
     1561 gtgtcgggca gcagaccggc tcgagaggtc agaggggatg agcggaagac gcgcgtgcct
     1621 tcctggtgca agcccgtggc ctcgcggccg gggatcgctt tgcggatgtg cattcgcagc
     1681 gtcaactcat attttgccag aacttttcac ttcgcgcact gaattaccat gcctgacccg
     1741 ccagcccggc cgttccggtg acgtgcttca cgtcctgccg gcacgccgtc cggagcccga
     1801 caagaaccgc gccgccccac gaaggcggcc gcccgaccag cgaacccgcc cgccatgccc
     1861 gaccacaaga ccgccctgcc gaccagccac gtcctcgtcg tcagcgccga cgccacctgg
     1921 cgcgacgacg tcgtcgccgg cctcgacgcc gccgccgcgc gcctggacaa cccgttcgcg
     1981 ctgcgcgccg tggcggtggc gcaagcgtcg tccgcgcccg agagggtgct cgcggacggg
     2041 gacgtgcaga tcgtcctgat cgaccatggc gaccacgggc cgggcgccgt ggccacgcac
     2101 caggacgtcg ccgagctcgc gcgcgggatc atcgcgctga ggcccgagct gtcgctgtat
     2161 gtcacgctgg aggacgacga gaacaagatg ctgctggagc agctcgcgag caacgtcgtg
     2221 cacggctact tctaccgcgg cgagcgcgac tacaacggct gggcgcgcat cctcgccgcg
     2281 gagatcgccg agaagtccgc gacgccgttc tacgacgagc tgcgccacta cgtgcgcatg
     2341 gccaaggaca gctggcacac gcccggccac gcgggcggcg actcgttcca gggcagcccg
     2401 tgggtgggcg acttccacga tttcgtcggc gagaacatgc tgcgcgccga cctctcggtc
     2461 tcggtcgaga tgctggactc gctgatgcac ccgaccggcg tcatcgcgca ggcccagcgc
     2521 ctggctgcgc aggccttcgg cgcgcgcaag acctacttcg cgaccaacgg cacgtcgacg
     2581 gccaacaagg tcatcttcca gacgctgctc gccccgggcg acaagctgct gctcgaccgc
     2641 aactgccaca agtcggtcca ccacggcgtg atcctgtccg gcgcgcggcc ggtgtacctg
     2701 gattcgtcgg tcaaccgcaa gtacgggctg ttcgggccgg tcccgcgcgc gacggtgctc
     2761 gaggcgatcg tcgcccaccc ggacgcgcgc gcgctgatcc tcacctcctg cacgtacgac
     2821 ggcctgcgct acgacctcgc gcccatcgtc gaggccgcgc atgccgcggg catcaaggtg
     2881 atcgtcgacg aggcctggta cggccacgcg cgcttccacc cggcgctgcg cccgaccgcg
     2941 ctggagagcg gcgccgacta ctgcacccag agcacgcaca aggtgctgtc cgccttctcg
     3001 caggcgagca tgatccacgt caacgacccg gcgttcgacg agcacctgtt ccgcgagaac
     3061 ttcaacatgc acgcctcgac gagcccgcag tacaacctga tcgcgagcct ggacgtggcg
     3121 cgcaagcagg cggtgatgga gggctaccgc ttgctcgacc gttcgctgaa gtacgcgcag
     3181 gagctgcgcg agcgcatcaa cgccaccggc gccttccgcg cgctggagct ggccgacctg
     3241 ctgcccgagg agctcgccgg cgacaacatc cagctcgacc cgaccaagat cacgatcgac
     3301 atctcgccct cgggcttctc ctcggacgag ctgcgcgagg agctgttcga gcgcttcaac
     3361 atccaggtcg agaagtcgac tcacaacacg ctcacgctgc tgctgacggt gggcacgacg
     3421 cgctccaagg tctcgcgcct gttcgacgcg ctgctgcggt tgtccaagga acgccgtccg
     3481 gcgcgggcga acctgcgcat gcccgagctg ccgcacttca cgcgcctcgc ctgcctgccg
     3541 cgcgacgcgt tctacgagta cggcgagcgc ctgccgctgc tcgacgaaca gggccagccc
     3601 aacgcggcgc tggaaggccg cgtctgctgc gaccagatca ccccgtaccc gcccggcatc
     3661 cccgtgctcg tgcctggcca ggtcatcacc gccgagatca tcggcttcct cacgcgcctg
     3721 atgcgcatgc agaagtccat cgagatgcac ggcctggcga cctgcgaggg cgaggagtgc
     3781 ctgcgcgtgc tgcggcccca cgagctcgac gcgctcgcgc agcggtcccg gctctgatca
     3841 ggcggtcgcc agcgcctcgt cgaggatcgc gacggcgcgg tcgatctccg cgtcggtgac
     3901 cgtcagcggc ggggcgatgc ggaagacgcc gcccatgccg gggagcttga cgatgttcat
     3961 gctcaggccg agcgccatgc agcggcgggc gacggcggcg ccgtagtcgt tcgcgggggc
     4021 cttggtcgcg cgatcctgca cgacctcgag accgagcatc agcccgcgac cgcgcacgtc
     4081 gccgacgcgc tcgtggcgct cctgcagcgc gagcaggccg tcgcgcaggc ggccgccggc
     4141 gacgcgcgcg cgctcgacga ggccatcgcg tcgcacgacg tccagcaccg tcaggcccac
     4201 cgccgcgggc agcgggtcgg agacgtgcgt cgtgtagagc aggtagccgc gctcgtgggc
     4261 gcgctcctcg atctcggcgg tcgtgatcgt cgcggccagc ggcaggcctg cgccgagcgt
     4321 cttcgacagc gtgaggatgt ccggcgcaac cccgtcgcgc tcgcacgcga acatcatgcc
     4381 cgtgcggccg acgccggtct gcgcctcgtc gacgatcagc agcatcccgc gctcctcgca
     4441 cttgcgcttg agcgccgcga ggtagccctc gggcaggtcg aggatgccgc cgctgctgag
     4501 gatcggctcg gcgatgaagg cggcgagcgc gccggtggac tggcgatcga cctggtcgaa
     4561 gccgtcgtcg agctcggcct gccagtcgtg gcggccgtcg cgcacgaagc gcgggcggta
     4621 cgcgttgggc gcggggatgg ccatggagcc gggcatcgcg ggcccgtagc cgcgccggcc
     4681 cgcgctgtag gtcgccgcgg cggcggcgcc ggtcatgccg tgccaggact gcgagaagcc
     4741 gacgatctcg tggccgccgg tgaccagctt ggccaggcgg atcgcggcct cgttggactc
     4801 ggcgcccgtg ctcaggagca ggcagcgctc gagccgcccg ggcgcgagct cggccagccg
     4861 ctgcgcgagg tcgacgaccg gccgcgacag cagcgtgctg ttcaggtgca tcagctggcc
     4921 ggccgaggcg cgcaccgtgg cgacgatgtc gggatgcgag tggccgagca gggcgctcat
     4981 ctggccggac gtgaagtcga ggatctcgcg gccgtcggca tcgaagagca ggctgcccgc
     5041 cgcgcgctcg atgatgaccg gctcgaagct gccgccgtag cgcacgaggt gccgccgggc
     5101 gcgttcccag aaggcggggt cgaggttgcg ggacgtcatg gcggctctcc ggtcggttgc
     5161 gatgccgcca cgataggact tgacgggtgt ttcgcacagc gaatactcgt gaaccgtcac
     5221 atcagcgata ttgataccca tgccggcctc gatcgacatc gaactcgccc gcagcttcgt
     5281 cgccatggcc gacgccggca ccctcgtggg cgcggcgggc cgcgtcgggc gcacgcagtc
     5341 cgcgctgagc atgcagttga agcgcctgga ggagaccgtc ggccgcacgc tgttcgatcg
     5401 ctcgccgcag ggcctgcggc tgacggcggc gggcgacctg ctgctgggcc acgcgacgcg
     5461 cctgctgcgc gtgcacgacg aggcgcttgc cgagctgtcg ggccgcggtc tggcgggccg
     5521 gctgcgcctg ggctgccccg aggactacgc cgtgcgcctg ctgcccgagt tgctgcgccg
     5581 tttcggcagc cagcatccgc gcgtggagtt cgaggtcgcc tgcgcgccga cgccgcgcct
     5641 gcgcgcgatg ctggcgcggc acgcgatcga cgtggcgctc gtctcgcagc cgcaggaggg
     5701 agaggacgcc ggcgcgatcc gcgtcgagcc cctggtgtgg gtcggccgcg ccgacgtcga
     5761 cccggccgcg ttcgacccgc tgccgctggc actgtccgag cccgaggcct tcgaccacca
     5821 ggcgccgctc gaggtgcttc gccggcgcgg tcgcgcctgg cggatcgcgc acgcgagccc
     5881 gcacctgctg ggcttgctct cgatggcgcg ctcggggctg gcgatcacgg tgctgacggg
     5941 caccgcggtg ccggccgac
//
LOCUS       DASVLN010000196         4569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_171409, whole genome shotgun sequence.
ACCESSION   DASVLN010000196 DASVLN010000000
VERSION     DASVLN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171409"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VF453_04490"
     CDS             complement(<1..563)
                     /locus_tag="VF453_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX7686941.1"
                     /translation="MDTPAATTSDPHGPRPSVRAVFLAFLRLGCTSFGGPVAHLGYFR
                     SEFVERRRWLDDATFTDLVALCQFLPGPASSQVGIAIGLLRAGWIGSLAAWLGFTLPS
                     AALLIAFGYGISRFADVARSGAVHGLKVVAVAVVAQAVWGMAKSLCPDRPRAALAIVA
                     ALLTLVVPGAVGQLGAIAACAIVGWSML"
     gene            complement(595..1497)
                     /locus_tag="VF453_04495"
     CDS             complement(595..1497)
                     /locus_tag="VF453_04495"
                     /inference="COORDINATES: protein motif:HMM:NF016293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HEX7686942.1"
                     /translation="MHDLPTPAVAPSWPDAHAALGEEYAAQWTRRAKYALRKLVTSRQ
                     RAEVMAFVAGSPAARTLLACAPRVFYPVMRKHLDRRFDTDRRARAVIESIRGLEQGMS
                     REACDEVCRGGSVVLGTLPDGTRIRLNLNRLTFDEGLWALDLSDEAGERLFTISFGFS
                     EPGTLMIGCVQGPHRHVDGLQSGRDLTKAAEGLRPAHLLMHLLRDCAAAWRVERIVGV
                     DEPFQSKGRWNHPAVGRKFDYDTFWTEVGGTKREDGNWDLPVSVPQRPLEDVPAKRRS
                     MYRKRYALLEALGVQARAAMGVRA"
     gene            complement(1535..3316)
                     /locus_tag="VF453_04500"
     CDS             complement(1535..3316)
                     /locus_tag="VF453_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEX7686943.1"
                     /translation="MPSDLHATSIARLLSEKTLRAHYQPIVRVDTGEVVAHESLIRLP
                     PGHPLRNPDELFRAAREQGLLVRTEQACLDEGLRTWKRRGAERLFLNLSAYAVIEMVD
                     RLGAAGVVRALEAAGVAPASLVVEITEHDHVGDVPRLIRAATNIRAYGLRFALDDFGD
                     GRSSLRLWAELRPDYVKIDKYFTKGIADNPVKVQTLRGLARFAEIFGTTLVAEGIETM
                     DELRVIRDLGLPLGQGFGLGRPAPEPALAIADEARAAIESSVISVLPELARAGGGDVT
                     IERLLTWVEPRPPDLSVNALAQQFAADDSLRAVALVADGRPVGLVNRQSFIDRCARPY
                     FRDLYGRQPCIEFANRTPLILDRHVGVDVMTEVLTSADQRYLTEGFIVTDDGRYAGLG
                     SGQQLVRLVTEVRIEAARHANPLTFLPGNIPISEHIRRLLEVGAPFAACYADMNDFKP
                     FNDHYGYWQGDEMIRLVARTLLAHCDPRRDFVGHVGGDDFVVLFQSADWEERCRAIVA
                     AFNERAPALFDAQALERGGIEAEDRHGVRRFFPFTTLALGVVPVRPGLFDKPEAVASA
                     AAAAKHQAKSARSGLFVERPADGRLPA"
     gene            3642..>4569
                     /locus_tag="VF453_04505"
     CDS             3642..>4569
                     /locus_tag="VF453_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX7686944.1"
                     /translation="MKLKHKIPGVFAGALALSLAAGLGSVLVVDRSLSTIRSDVMRQV
                     DDERAITSLSGHFKAQLLEWKDTLLRGSDVHLLDKHWNGFLRQQQAVAEGAQALRGRL
                     DSAELNAELERFVAARRRMAEGYAQGFDKFKATGFDSSVGDMAVRGIDDEPARLLDDL
                     AARIAERRNHVAAQAFDAGVRAIGFAVAVMVGAGVLGLALGLALGRRVVRPLLSATQV
                     ARAVAVGDLARRIEVEGDDETAALLRALAEMQAQLRGVVGSVRESASSVEDASAGIAQ
                     GNVDLSRRTGQQSSALQVTASTMEQLGATASNS"
BASE COUNT          640 a         1613 c         1727 g          589 t
ORIGIN      
        1 agcatcgacc agccgacgat cgcgcaggcg gcgatcgcgc cgagctgccc gacggcgccc
       61 ggcacgacga gcgtgagcag cgccgcgacg atggccagcg cggctcgcgg ccggtcgggg
      121 cacagcgact tcgccatgcc ccacaccgcc tgcgcgacca ccgcaacggc gacgaccttg
      181 aggccatgca ccgcgcccga gcgcgccacg tcggcgaagc gcgagatgcc gtagccgaac
      241 gcgatcagca gcgccgcgga cggcagcgtg aagccgagcc acgccgcgag cgatccgatc
      301 cagccggcgc gcagcaggcc gatcgcgatc ccgacctggc tgctggccgg cccgggcagg
      361 aactggcaca gcgcgacgag gtcggtgaag gtcgcatcgt cgagccagcg gcggcgctcg
      421 acgaactcgg agcggaagta gcccaggtgc gccacggggc cgccgaagga cgtgcagccc
      481 aggcgcagga acgcgaggaa gacggcccgg acggacggtc gcgggccgtg cggatccgac
      541 gtcgtcgcgg cgggcgtgtc catggggtcg tcgacggcgc cagcgatgcg gcgttcaggc
      601 ccgcacgccc atcgcggccc gcgcctgcac gccgagcgcc tcgagcagcg cataccgctt
      661 gcggtacatc gagcggcgct tcgccggcac gtcctcgagc ggccgctgcg gcacgctgac
      721 cggcaggtcc cagttgccgt cctcgcgctt cgtgccgccg acctccgtcc agaaggtgtc
      781 gtagtcgaac ttgcgtccca cggcgggatg gttccagcgg cccttggact ggaagggctc
      841 gtccacgccg acgatgcgct cgacgcgcca cgcggcggcg cagtcgcgca gcaggtgcat
      901 cagcaggtgg gccgggcgca ggccctccgc ggccttcgtg aggtcgcgcc cggactgcag
      961 cccgtcgaca tggcggtgcg ggccctggac gcagccgatc atcagcgtgc ccggctccga
     1021 gaagccgaag ctgatcgtga acaggcgctc gccggcctcg tcggacaggt cgagcgccca
     1081 cagcccttcg tcgaaggtca ggcggttgag gttgaggcgg atgcgggtgc cgtccggcag
     1141 cgtcccgagc accacgctgc cgccgcggca gacctcgtcg caggcctcgc gcgacatccc
     1201 ctgctcgagg ccgcggatgc tctcgatgac ggcgcgcgcg cggcggtcgg tgtcgaagcg
     1261 gcggtcgaga tgcttgcgca tcaccgggta gaacacccgc ggcgcgcagg ccagcagcgt
     1321 gcgcgcggcc ggcgagccgg ccacgaacgc catgacctcg gcgcgctgcc gcgaggtgac
     1381 gagcttgcgc agcgcgtact tcgcgcggcg cgtccattgg gcggcgtatt cctcgccgag
     1441 cgcggcgtgg gcgtcgggcc aggacggcgc gacggcggga gtcgggagat cgtgcatgga
     1501 attcgttcgc cccggcggcg agggcgcgat cttctcatgc cggcaggcga ccgtccgcgg
     1561 ggcgctcgac gaacaggccc gagcgcgcgg acttcgcctg gtgcttggcc gccgccgcgg
     1621 ccgacgcgac cgcctccggc ttgtcgaaca gcccgggccg cacgggcacg acgcccagcg
     1681 cgagcgtcgt gaacgggaag aaccggcgca ccccgtggcg atcctcggcc tcgatgccgc
     1741 cgcgctcgag cgcctgggcg tcgaacagcg cgggcgcgcg ctcgttgaag gccgcgacga
     1801 tcgcgcggca gcgctcctcc cagtccgcgc tctggaacag cacgacgaag tcgtcgccgc
     1861 cgacgtggcc cacgaagtcg cgccgcgggt cgcagtgcgc gagcagcgtg cgggcgacca
     1921 ggcggatcat ctcgtcgccc tgccagtagc cgtagtggtc gttgaacggc ttgaagtcgt
     1981 tcatgtcggc gtagcacgcc gcgaagggcg cgccgacctc cagcaggcgg cggatgtgct
     2041 cgctgatcgg gatgttgccg ggcaggaagg tgagcggatt ggcgtggcgc gccgcctcga
     2101 tgcgcacctc ggtgaccagg cggacgagct gctggccgct gcccagcccg gcgtagcggc
     2161 cgtcgtcggt gacgatgaat ccctcggtca ggtagcgctg gtcggccgac gtgagcacct
     2221 ccgtcatcac gtcgacgccg acgtggcggt cgaggatcag gggcgtccgg ttcgcgaact
     2281 cgatgcaggg ctggcggccg tagaggtcgc ggaagtaggg ccgggcgcag cggtcgatga
     2341 aggactgccg gttgacgagc cccaccggcc ggccgtcggc caccagcgcc accgcgcgca
     2401 gcgagtcgtc ggcggcgaac tgctgcgcga gcgcgttgac gctcaggtcg ggcggccgcg
     2461 gctcgaccca ggtcagcagg cgctcgatgg tgacgtcgcc cccgcccgcg cgcgcgagtt
     2521 cgggcagcac cgagatcacg ctgctctcga tcgccgcgcg tgcctcgtcg gcgatcgcca
     2581 gcgcgggctc gggcgcgggc cggcccaggc cgaagccctg gcccagcggc aggccgaggt
     2641 cgcggatgac gcgcagctcg tccatcgtct cgatgccctc ggcgaccagc gtcgtgccga
     2701 agatctccgc gaagcgcgcg aggccgcgca gcgtctgcac cttcaccggg ttgtcggcga
     2761 tgcccttggt gaagtacttg tcgatcttca cgtagtcggg ccgcagctcg gcccacaggc
     2821 gcaggctcga tcggccgtcg ccgaagtcgt cgagcgcgaa gcgcaggccg taggcgcgga
     2881 tgttcgtggc ggcgcggatc aggcgcggca cgtcgccgac gtggtcgtgc tccgtgatct
     2941 cgacgaccag cgacgccggc gcgacgcccg ccgcctccag cgcacgcacg acgcccgcgg
     3001 cgcccaggcg gtcgaccatc tcgatcacgg cgtacgcgct caggttcagg aacaggcgct
     3061 cggcgccgcg ccgcttccac gtgcgcaggc cctcgtcgag gcaggcctgc tcggtgcgca
     3121 cgagcaggcc ctgctcgcgc gcggcgcgga acagctcgtc ggggttgcgc agcgggtggc
     3181 cgggcggcag ccggatcagg ctctcgtggg cgacgacctc gccggtatcg acgcggacga
     3241 tgggctggta gtgcgctcgc agcgtcttct cgctcagcag gcgagcgatc gaggtcgcgt
     3301 gcaggtccga tggcattcag gggaatctcc gtggtcgcgc cgccgccgtt tgcgtcgcgg
     3361 ccggtccgcg ccacccgtgg aactgtatcg agccgggctt cgcgtgcgcg atgcctgcac
     3421 aaaagtagcc gcgttcgcgg gaggaatgcg cgcctgcgca cgagatgtcc tgacccgctc
     3481 cgcgcgcctg ggcggtcgtc acgagggaag cggttccagc cgtgtgacga tcgtgtgaat
     3541 ccggcccatg cccgagggat cgcgggggca tgctcaagcc cgcggggacg gggtcgaaaa
     3601 gctcgcggtc ggccgcggcg cggcccctcg aaaacttcgt catgaaactg aaacacaaga
     3661 tccccggcgt cttcgccggc gccctcgcgc tctcgctggc cgccggcctc ggcagcgtcc
     3721 tcgtcgtcga tcgctcgctg tccacgatcc gctcggacgt gatgcggcag gtcgacgacg
     3781 agcgcgcgat cacgagcctg tcgggccact tcaaggcgca gctgctggaa tggaaggaca
     3841 cgctgctgcg cggctcggac gtgcacctgc tggacaagca ttggaacggg ttcctgcggc
     3901 agcagcaggc cgtggccgag ggcgcgcagg ccctgcgcgg gcgcctggac agcgcggagc
     3961 tcaacgccga gctcgagcgc ttcgtcgcgg cgcgccggcg catggccgag ggctacgcgc
     4021 agggcttcga caagttcaag gccacgggct tcgattcgtc ggtcggcgac atggccgtgc
     4081 gcggcatcga cgacgagccc gccaggctgc tggacgacct cgccgcgcgc atcgccgagc
     4141 gccgcaacca cgtcgcggcg caggccttcg acgccggcgt gcgggcgatc gggttcgccg
     4201 tggccgtgat ggtcggcgcc ggcgtgctgg ggctcgcgct cgggctcgcg ctcggccgcc
     4261 gcgtcgtgcg tccgctgctg agcgcgacgc aggtcgcccg ggccgtcgcg gtgggtgacc
     4321 tggcgcgccg catcgaggtc gagggcgacg acgagacggc ggcgctgctg cgcgccctgg
     4381 ccgagatgca ggcccagctg cgcggcgtcg tcggcagcgt gcgcgagagc gcgagcagcg
     4441 tcgaggacgc gagtgccggc atcgcgcagg gcaacgtcga cctgagccgc cgcaccggcc
     4501 agcagtcgtc ggcgctgcag gtgacggcgt cgacgatgga acagctcggg gccaccgcga
     4561 gcaacagcg
//
LOCUS       DASVLN010000197         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_63343, whole genome shotgun sequence.
ACCESSION   DASVLN010000197 DASVLN010000000
VERSION     DASVLN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63343"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VF453_04510"
     CDS             <1..1143
                     /locus_tag="VF453_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEX7686945.1"
                     /translation="AATAYSAGATVTENGVTYKANWWTQGDDPATHSGPTGSGQPWSV
                     TTSCSGSPTPPPPPPPPPPPPPPPGPPTPAGFVFGSYKDVTINMDWNVDQISTTIGGS
                     RQPVLNAMPARQPSMTWAFASGECGSENWGGVSPSALVAQNLSQWVAAGKKYIVSTGG
                     ANGVFTCGSDAGFEKFIQTYNSSSLQGIDFDIEGGQSQDVINNLVARVKTAQANHPNL
                     RFSFTLATLGGNAPQSLGAIGVNVMSAIKSAGLSGYIINLMTMDYGSANPSNCTIGGN
                     GQCDMAQSAINAAINLHNYYGVPYSQIELTPMVGGNDTQGETFTLANVDTVSSWVKAN
                     GLAGVHFWSLDRDTDCAPGSSSPTCNSYGQAGNWGFTNRFISDLGL"
     gene            complement(1240..2808)
                     /locus_tag="VF453_04515"
     CDS             complement(1240..2808)
                     /locus_tag="VF453_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7686946.1"
                     /translation="MSHPPVRKSLSVARRLAWGFGALVGLSLVSSVVAGWQAGQIAAK
                     VQRVVEFNNAVSDKVGHLRNAMDEMSIQARSVALMTEMKSINAEVDRFKAAKTRYQAD
                     EKALQALVAGAGGTDDERRIVKDVVALSTVTIPLVEEATNQAADGNNVEAALGLTNRV
                     APKETAWREALANLQKVVAADDAAAVSGARAAEAAMAGLLVAFAVAAVAIGSLVGWRA
                     IRSVKGPLDRAMQFARRVAEGDLSSEAQPERADELGLLLESLNGMQAKLRALVGHIRE
                     SAESIGTASKEVADGNQDLSSRTEQTAGNLQHAASAMTQLTGNVRQSADAAAQANGLA
                     MSASEVARRGGRAVSEVVETMNGINESSKKISDIIGVIDGIAFQTNILALNAAVEAAR
                     AGEQGRGFAVVASEVRSLAQRSAGAAKEIKSLIGASVERVETGARQVAEAGQTMREIE
                     SSVERVTAIISEISAASAEQSSGIGQVSGAVSHLETMTQQNAALVEESAAAAESLRSQ
                     AAGLNTLVGAFRLA"
     gene            3226..3750
                     /locus_tag="VF453_04520"
     CDS             3226..3750
                     /locus_tag="VF453_04520"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7686947.1"
                     /translation="MSKSIFARAAIGAALLATAGIASATNVGTFTVDAMANSIEGGSA
                     FDTGVTLHAGDTLTISVPTTDIWNNSYGDPHYDANANGNTVQGYFEYGNLSTNIGSLV
                     GQIGNGDYFKVGTSYTGTVSSTGDLKFFYWDSDAYNNVGAVHAAVSAVPEPATFALMG
                     LGLGLLGLSRRRKI"
     gene            complement(3839..>4250)
                     /locus_tag="VF453_04525"
     CDS             complement(3839..>4250)
                     /locus_tag="VF453_04525"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7686948.1"
                     /translation="APALPSAGDLDAVLAALAGELRRRAPANERLQWIRASVGQMIKL
                     IPVDEVLYFRSDEKYTLVVWVGGEALIRKSIRELLDGLDPETFIQVHRSTIVNLRFVA
                     QVVKGPNETAEVQLKGRPETLAVSRNHVHWFRQY"
BASE COUNT          654 a         1556 c         1417 g          623 t
ORIGIN      
        1 gcggcaaccg cgtacagcgc cggcgccacg gtcaccgaga acggcgtgac ctacaaggcc
       61 aattggtgga cgcagggcga cgatcccgcg acccacagcg gcccgaccgg cagcggccag
      121 ccctggagcg tcaccaccag ctgcagcggc tcgcccacgc cgcctccccc gcccccgcct
      181 ccgccgcccc cgccgccccc gccggggccg ccgacgccgg ccggcttcgt gttcgggtcc
      241 tacaaggacg tgacgatcaa catggactgg aacgtcgacc agatctcgac gaccatcggc
      301 ggctcgcgcc agccggtgct gaacgccatg cccgcgcgac agccgtcgat gacctgggcg
      361 ttcgcctcgg gcgagtgcgg ctcggagaac tggggcggcg tgtcgccctc ggcgctggtt
      421 gcgcagaacc tcagccagtg ggtcgcggcc gggaagaagt acatcgtctc gaccggcggc
      481 gccaacggcg tcttcacctg cggctcggac gccggcttcg agaagttcat ccagacctac
      541 aactcttcca gcctgcaggg catcgacttc gacatcgagg gcggccagtc gcaggacgtg
      601 atcaacaacc tcgtcgcgcg ggtgaagacg gcgcaggcca accatccgaa cctgcgcttc
      661 agcttcacgc tggccacgct gggcggcaac gcgccgcaga gcctgggcgc gatcggggtc
      721 aacgtcatgt cggcgatcaa gtcggccggg ctgtcgggct acatcatcaa cctcatgacg
      781 atggactacg gcagcgcgaa cccgtcgaac tgcacgatag gcggcaacgg ccagtgcgac
      841 atggcgcagt cggcgatcaa cgcggcgatc aacctgcaca actactacgg ggtgccgtat
      901 tcgcagatcg agctgacgcc gatggtcggc ggcaacgaca cgcagggcga gaccttcacg
      961 ctcgccaacg tcgacaccgt gtcgtcctgg gtcaaggcca acgggctggc cggcgtgcac
     1021 ttctggtcac tcgaccgcga cacggactgc gcgcccggca gctcctcgcc gacctgcaac
     1081 agctacggcc aggccggcaa ctggggcttc acgaaccggt tcatctcgga cctggggctg
     1141 tgatgcgctg agccgcctgc gccgcaggcg cgaaagcctg ctccgctcgc gcggggcagg
     1201 cttcattgcg tgcgcgggcg cggccctggc cgcgcgcgat caggccaggc ggaacgcgcc
     1261 gaccagcgtg ttcaggccgg cggcctgcga gcgcagcgat tccgcggcgg cggcgctctc
     1321 ttcgacgagc gcggcgttct gctgcgtcat cgtctcgagg tgcgacacgg cgcccgagac
     1381 ctggccgatg cccgaggact gttccgccga ggcggcgctg atctccgaga tgatggccgt
     1441 cacgcgctcg acgctggact cgatctcgcg catcgtctgg ccggcctcgg cgacctggcg
     1501 cgcgcccgtc tcgacgcgtt ccaccgacgc gccgatcagc gacttgatct ccttggccgc
     1561 gcccgccgag cgctgcgcca gcgagcgcac ctccgaggcc acgaccgcga agccgcgccc
     1621 ctgctcgccc gcgcgcgcgg cctcgaccgc ggcgttcagc gccaggatgt tggtctggaa
     1681 cgcgatgccg tcgatgacgc cgatgatgtc cgagatcttc ttcgagctct cgttgatgcc
     1741 gttcatcgtc tcgaccacct cggagaccgc ccgcccgccg cggcgcgcga cttccgacgc
     1801 cgacatcgcc aggccgttgg cctgcgccgc cgcgtccgcg ctctggcgca cgttgccggt
     1861 gagctgggtc atggcgctcg cggcatgctg caggttgccg gccgtctgtt cggtgcgcga
     1921 gctcaggtcc tggttgccgt cggcaacctc cttgctggcc gtgccgatcg actcggccga
     1981 ctcgcggatg tggccgacca gcgcgcgcag cttggcctgc atgccgttca gggactcgag
     2041 gagcaggccc agctcgtcgg cgcgctcggg ctgcgcctcg gacgacaggt cgccctcggc
     2101 gacgcggcgc gcgaactgca tcgcgcgatc gagcgggccc ttgacgctgc ggatcgcgcg
     2161 ccagcccacc agcgagccga tcgccaccgc ggcgaccgcg aacgccacca gcaggcccgc
     2221 catcgcggcc tcggccgcgc gggcgccgga gacggcggcc gcgtcgtccg cggcgacgac
     2281 cttctgcagg ttggcgagcg cctcgcgcca cgcggtctcc ttgggcgcga cgcggttggt
     2341 caggccgagc gccgcctcga cgttgttgcc gtccgcggcc tggttcgtcg cctcctcgac
     2401 cagcgggatc gtcaccgtcg acagcgcgac cacgtccttg acgatgcggc gctcgtcgtc
     2461 cgtgccgccg gcgccggcga ccagcgcctg cagcgccttc tcgtcggcct ggtagcgcgt
     2521 cttggccgcc ttgaagcggt cgacctcggc gttgatcgac ttcatctcgg tcatcagggc
     2581 gacgctgcgc gcctggatgc tcatctcgtc catcgcgttg cgcaggtggc cgaccttgtc
     2641 ggacacggcg ttgttgaact cgacgacgcg ctgcaccttg gcggcgatct gcccggcctg
     2701 ccagccggcg accaccgagg acaccagcga caggccgacg agcgccccga acccccaggc
     2761 gaggcgacgg gcgacggaca ggctcttgcg gacaggggga tgggacattc gaggactccg
     2821 ggacagggtt tccccggagc tatcggccct gcgccggcat tcttgagtcc ggcggacggc
     2881 gcgcggtgca cccgtgccgc agaaatcacg cgatggcgcg ggcggcgcct ggcacggctc
     2941 gccgctgccg acgtgatcgc accgaagtga tcctcccgag ggccatcggc cgatcggcgc
     3001 ccggcttgag cccttgaaaa agcgcaatca gggaaaatcg ggaatagatc acggaaatca
     3061 aagtggcagg agcgatatcg gggactcatg cggcaccgca gcaatcccgc tcgacggtac
     3121 agtaacggca cgggatggcc gaacggtttt cccgagcact ccctgcctgt catggaaggg
     3181 ggaggactcc aaccgactcc aaccgacaac aacgggaacc ttgtgatgag caagagcatc
     3241 ttcgcgcgcg ccgccatcgg cgccgccctg ctggccacgg ccggcatcgc ctccgccacc
     3301 aacgtcggca ccttcacggt cgacgcgatg gccaactcga tcgagggcgg ctccgccttc
     3361 gacacgggcg tcacgctgca cgccggcgac acgctgacga tctcggtgcc gaccaccgac
     3421 atctggaaca actcctacgg cgacccgcac tacgacgcga acgccaacgg caacaccgtc
     3481 cagggctact tcgagtatgg caacctgagc acgaacatcg gctcgctggt cggccagatc
     3541 ggcaacggcg actacttcaa ggtcggcacc tcgtacaccg gcacggtctc gtccaccggc
     3601 gacctgaagt tcttctactg ggacagcgac gcgtacaaca acgtcggcgc cgtgcacgcc
     3661 gccgtctcgg ccgtgccgga acccgcgacc ttcgccctga tgggcctggg cctgggcctc
     3721 ctcggcctga gccgccgccg caagatctga ggccgcgcgg cgcccggcgc cgccctcctc
     3781 gactgcaaag ggtgcccgcg ggcacccttt ttcattgacg cgtccgcggt tcccggcttc
     3841 agtactggcg aaaccagtgc acgtggttgc ggctgacggc cagcgtctcg ggacgcccct
     3901 tcagctgcac ctccgccgtc tcgttcggtc ccttgacgac ctgcgcgacg aagcgcaggt
     3961 tgacgatggt cgagcggtgt acctggatga aggtctccgg atcgaggccg tcgagcagct
     4021 cgcggatgga cttgcggatc agcgcctcgc cgccgaccca gacgaccagc gtgtacttct
     4081 cgtcggagcg gaagtacagc acctcgtcga ccgggatcag cttgatcatc tgccccaccg
     4141 acgcgcggat ccactgcagc cgctcgttgg ccggcgcgcg ccggcgcagt tcgccggcca
     4201 gcgccgcgag cacggcgtcc aggtcgccgg ccgacggcaa cgccggggcg
//
LOCUS       DASVLN010000198         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_224562, whole genome shotgun sequence.
ACCESSION   DASVLN010000198 DASVLN010000000
VERSION     DASVLN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224562"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2302)
                     /locus_tag="VF453_04530"
     CDS             complement(<1..2302)
                     /locus_tag="VF453_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEX7686949.1"
                     /translation="MEKILLQIAAELNVRPAQVNAAVTLLDGGATVPFIARYRKEVTD
                     NLDDAQLRELESRLAYLRELEDRREAVLRSIEEQGKLTPELAAAIAAAPTKQELEDLY
                     LPYKQKRRTKGMIAREAGLEPLADRLFADPSLDPHAEAAAFVNADAGFADAFAVLDGV
                     RDLLSERWAEDAALVGTLREWLWEEGLLQSKKTDGKDEHNADVAKFRDYFDYAEPIRT
                     VPSHRALAVFRGRTQEILDAKLVLDEELVPGQPSLAEGRIARHLGWSHAKRPADDLIR
                     RTVSWTWKVKLSLSLERDLFARVREEAEKVAIRVFAENLRDLLLAAPAGKRVVMGLDP
                     GIRTGVKVAVVSDTGKVLATHTVYPHEPRCDWEGSLHALGRLVAQHGVNLIAIGNGTA
                     SRETDKLAGDLIKRVQQLAPDVKIDKVVVSEAGASVYSASEFASKELPELDVSLRGAV
                     SIARRVQDPLAELVKIDPKSIGVGQYQHDVNQSELARTLDAVVEDCVNKVGVDLNTAS
                     APLLARVSGLTQAVAGSIVRWRDANGAFATRAQLLEVAGLGPKTFEQAAGFLRIRDGA
                     NPLDMTGVHPETYPVVERMLAQAGRPIQEVMGRSDVLKSLRPDAFADERFGAITVRDI
                     LVELEKPGRDPRPDFKVARFNDGVEDIKDLQPGMMLEGTVSNVAQFGAFVDLGVHQDG
                     LVHVSQLSNKFVNDAREVVKTGDIVKVKVLEVDLARQRIALTMKLDAQPVPKGARNAD
                     NSFRPAARNERAGGGQRGGPSSAPAGQ"
BASE COUNT          328 a          893 c          882 g          406 t
ORIGIN      
        1 cctgccccgc cggcgcggac gagggcccgc cgcgttggcc gccgcccgcg cgctcgttgc
       61 gcgccgccgg gcggaagctg ttgtccgcgt tgcgcgcgcc cttgggcaca ggctgcgcgt
      121 ccagcttcat cgtgagcgcg atgcgctggc gcgcgaggtc gacctcgagc accttgacct
      181 tgacgatgtc gcccgtcttg acgacctcgc gcgcatcgtt gacgaacttg ttggacagct
      241 ggctcacgtg cacgaggccg tcctggtgca cgccgaggtc gacgaacgcg ccgaactgcg
      301 ccacgttgct gacggtgcct tccagcatca tgccgggctg caggtccttg atgtcctcga
      361 cgccgtcgtt gaagcgcgcg accttgaagt cgggacgcgg gtcgcggcca ggcttctcga
      421 gctcgacgag gatgtccctg accgtgatgg cgccgaacct ctcgtcggcg aatgcgtcgg
      481 ggcgcaggga cttcagcacg tcgctgcggc ccatcacctc ctggatcggc cgccccgcct
      541 gcgcgagcat gcgttcgacg accggatagg tctccgggtg cacgccggtc atgtccagcg
      601 ggttggctcc gtcgcggatg cgcaggaagc cggcggcctg ctcgaaggtc ttcgggccga
      661 ggcccgcgac ctccagcagc tgcgcccggg tcgcgaacgc gccgttggcg tcgcgccagc
      721 gcacgatgct gcccgcgacc gcctgcgtca gccccgacac gcgcgccagc agcggtgcgg
      781 acgcggtgtt caggtcgacg ccgaccttgt tcacgcagtc ctcgacgacc gcgtccagcg
      841 tcctggcgag ctcgctctgg ttgacgtcgt gctggtactg gccgacgccg atgctcttcg
      901 ggtcgatctt gaccagctcg gcgagcggat cctgcacccg gcgcgcgatc gagacggcgc
      961 cgcgcaggct cacgtcgagc tcgggcagtt ccttgctcgc gaactccgag gccgagtaca
     1021 ccgacgcgcc cgcctcgctc acgaccacct tgtcgatctt cacgtccggc gcgagctgct
     1081 gcacgcgctt gatcaggtcg cccgcgagct tgtcggtctc gcggctggcg gtgccgttgc
     1141 cgatcgcgat caggttgacg ccgtgctgcg cgacgaggcg gccgagcgcg tgcagcgaac
     1201 cctcccagtc gcagcgcggc tcgtgcgggt agacggtgtg ggtcgcgagc accttgccgg
     1261 tgtcgctcac caccgcgacc ttgacgcccg tgcggatccc ggggtcgagg cccatcacga
     1321 cgcgcttgcc cgccggcgcg gccagcagca ggtcgcgcag gttctcggcg aagacgcgga
     1381 tggcgacctt ctcggcctcc tcgcgcacgc gggcgaacag gtcgcgctcc aggctcagcg
     1441 acagcttgac cttccacgtc cacgacaccg tgcgtcggat caggtcgtcg gccgggcgct
     1501 tcgcgtgcga ccagccgagg tggcgcgcga tgcggccttc ggcgagcgag ggctgcccgg
     1561 gcaccagctc ttcgtcgagc accagcttgg cgtcgaggat ctcctgcgtg cggccgcgga
     1621 agacggcgag cgcgcggtgc gacggcacgg tcctgatcgg ctcggcatag tcgaagtagt
     1681 cgcggaactt ggcgacgtcg gcgttgtgct cgtccttgcc gtcggtcttc ttcgattgca
     1741 gcaggccttc ctcccacaac cactcgcgca acgtgccgac gagcgccgcg tcctcggccc
     1801 agcgctcgga cagcaggtcg cgcacgccgt cgaggaccgc gaacgcgtcc gcgaagccgg
     1861 cgtccgcatt gacgaacgcc gccgcctcgg cgtgcggatc cagcgacggg tcggcgaaga
     1921 gcctgtcggc gagcggctcg aggccggcct cgcgggcgat catgcccttg gtgcgccgct
     1981 tctgcttgta cggcaggtag agatcctcga gctcctgctt ggtcggcgcg gcggcgatcg
     2041 ccgcggcgag ctcgggggtc agcttgccct gctcctcgat gctccgcagg acggcctcgc
     2101 gccggtcctc gagctcgcgc aggtaggcca ggcgcgactc gagctcgcgc agctgggcgt
     2161 cgtcgaggtt gtcggtgact tccttgcggt aacgcgcgat gaaaggcacc gtggcgccgc
     2221 catccagcag cgtgaccgcc gcgttcacct gggccggccg gacattcagc tcagccgcga
     2281 tttgaagaag gatcttttcc aaggccgttc cacacgccaa tgcatcgaaa gcgcgcgagt
     2341 gtgccatagc gatccggacg tccccggcgt cggcggcggc gtcggcggcg gcgtcggcgc
     2401 cccgccggtt gtggtagggc cacggttacg gcgcgggtac accggcgtgg caagcccgcc
     2461 cgtgacatcc ggttacccga cggtcgcgcc cgggccatga aatccaggc
//
LOCUS       DASVLN010000199         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_63428, whole genome shotgun sequence.
ACCESSION   DASVLN010000199 DASVLN010000000
VERSION     DASVLN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63428"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..775
                     /locus_tag="VF453_04535"
     CDS             194..775
                     /locus_tag="VF453_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX7686950.1"
                     /translation="MEHTLPPLPYAHDALQPHMSRETLEYHHDKHHNAYVTTLNNLQK
                     GTEFESLPLEEIIKKASGPIYNNAAQIWNHTFFWNCMKPNGGGEPKGALADAINAKWG
                     SFAAFKEAFQKSAVGNFGSGWTWLVKKADGSVDIVNMGAAGTPLTTGDKALFTVDVWE
                     HAYYIDHRNLRPKFVETYLNSLANWDFAEANFA"
     gene            1054..2178
                     /locus_tag="VF453_04540"
     CDS             1054..2178
                     /locus_tag="VF453_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7686951.1"
                     /translation="MQVKLNMIVAAAAALCAGAVSAQDVVRIGQVGPVSGPQAHYGKD
                     EQNGARMAIEDLNKQNLTIGGKKVKFELVAEDDAADPKQGTAAAQKLCDQKVNGVVGH
                     LNSGTTIPASKIYHECRIPQISPSATNPAYTKQGFDTAFRLLANDNALGSGLALYAAD
                     ALKLKRVAVIDDRTAYGQGVAEVFKKSAQARGIQIVSEQYTNDKATDFMSILTAIKAK
                     NPDGIFYGGMDPQAGPMLRQIDQLGMNVKFFGGDGICTEKLIELAAGAKELGNVVCAQ
                     GGVSIEKMPSGVEWKKRYDAEFPGEFQVYSPYTYDAVGVLVDAMKRANSTDPKVYLPF
                     VGKTNYKGVTTTVQFEPDGELKNPAMTFYAYKDGKQVALN"
     gene            2457..2780
                     /locus_tag="VF453_04545"
     CDS             2457..2780
                     /locus_tag="VF453_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686952.1"
                     /translation="MDKPLAIVAQDAAPEDTATARRRFNWIAAIFLLACGVSGVFAAV
                     LVAFVFRDAIGEPIGLLLAASLVAGVVTTWLLYQLGKQRLCDRAIVMGGGIASAVMCS
                     MMFLD"
BASE COUNT          514 a          985 c          951 g          444 t
ORIGIN      
        1 acgctcgacc tcggcgagcc cgacggcgac ctctgacgca cggcggggca ggggcgcacg
       61 cccgatagcc ctggcggcgc gattgccgcg gatcgggtgc ctacaatgcc ccgcgagggt
      121 cggaccgcca ggacggtcgc gtacctcaca gaaccaccga cgaaagctcg ttcgccaaga
      181 aaggacaaac ctcatggaac atacgctgcc tcccctgccg tacgcgcacg acgcgctcca
      241 gccgcacatg agccgcgaga cgctcgagta ccaccacgac aagcaccaca acgcctacgt
      301 cacgacgctc aacaacctgc agaagggcac cgagttcgag tcgctgccgc tcgaggagat
      361 catcaagaag gcgtcgggcc cgatctacaa caacgcggcc cagatctgga accacacgtt
      421 cttctggaac tgcatgaagc ccaatggcgg cggcgagccc aagggcgcgc tggccgacgc
      481 gatcaacgcg aagtggggct cgttcgcggc cttcaaggaa gcgttccaga agtcggcggt
      541 cggcaacttc ggctcgggct ggacctggct cgtgaagaag gccgacggct ccgtcgacat
      601 cgtcaacatg ggtgccgccg gcacgccgct gaccacgggc gacaaggccc tgttcacggt
      661 cgacgtgtgg gaacacgcgt actacatcga ccaccgcaac ctgcgcccga agttcgtcga
      721 gacctacctg aactcgctcg cgaactggga cttcgcggaa gccaacttcg cctgacccgc
      781 gagcctttcg gaaccctgcg aggcggcctt cgggccgctt ttttgttcgc gcgcgcgggt
      841 ttggtctgtc aacgcgcgtg gggtccattg cgttcggcgc gccgttggac gcctgcggct
      901 gaagatccac cgtccgagac attgagtacc gtcattcatt cggggaaatg tggttgatcc
      961 cgatagggct tcgggaatgt tttggagtat ctttgcgccc gttgaggtca tccacacgtc
     1021 acactcaact ttcccccaac cggaggtttg tacatgcaag tgaaactgaa catgatcgtc
     1081 gccgcggccg ctgcgctgtg cgccggcgcg gtctcggcgc aggacgtcgt ccgcatcggc
     1141 caggtcggtc ccgtctcggg cccgcaggcc cactacggca aggacgagca gaacggcgct
     1201 cgcatggcga tcgaggatct gaacaagcag aacctgacga tcggcggcaa gaaggtcaag
     1261 ttcgaactgg tcgcggaaga tgacgcggcc gatccgaagc aaggcaccgc cgccgcacag
     1321 aagctgtgcg accagaaggt caacggcgtc gtcggccacc tgaactcggg caccacgatc
     1381 ccggcctcga agatctacca cgagtgccgc atcccgcaga tcagcccgtc ggccacgaac
     1441 ccggcctaca cgaagcaggg cttcgacacc gcgttccgcc tgctggccaa cgacaacgcc
     1501 ctgggctcgg gcctggcgct gtacgccgcc gacgcgctga agctcaagcg cgtggccgtc
     1561 atcgacgacc gcaccgccta cggccagggc gtcgccgagg tgttcaagaa gtcggcgcaa
     1621 gcccgtggca tccagatcgt gagcgagcag tacacgaacg acaaggccac cgacttcatg
     1681 tcgatcctga ccgccatcaa ggcgaagaac ccggacggca tcttctacgg cggcatggat
     1741 ccgcaagccg gcccgatgct gcgccagatc gaccagctgg gcatgaacgt caagttcttc
     1801 ggcggcgacg gcatctgcac cgagaagctg atcgagctgg ccgccggcgc caaggagctg
     1861 ggcaacgtgg tgtgcgcgca gggcggcgtc tcgatcgaga agatgccttc cggcgtggag
     1921 tggaagaagc gctacgacgc cgagttcccg ggcgagttcc aggtctactc gccgtacacc
     1981 tacgacgcgg tgggcgtgct ggtcgacgcg atgaagcgcg ccaactccac cgacccgaag
     2041 gtctacctgc cgttcgtcgg caagaccaac tacaagggcg tgacgaccac ggtgcagttc
     2101 gagccggacg gcgagctgaa gaatccggcg atgacgttct acgcctacaa ggacggcaag
     2161 caggtcgccc tgaactgacc gccgcgtcat ccggttcgcc gggacgggaa gggcgccgcg
     2221 aggcgccctt tttcgttgcg tggccggttc gtcgtcgcgc ggcggccgcg gggatccgcc
     2281 ggcctctgcc ggggcgcgtc gattgccgcg tcgtcggcag gcggcggcac ttcggggcat
     2341 tcctcgtccg gatcgacgca aaccctgacg ccggccccgc ggcgcagcgc tacgatctcg
     2401 tcgtggcgcc gcccgggagc ccgtgtgcgc cgcggtgccg tcgaaggaga accgccatgg
     2461 acaagccact cgcgatcgtt gcccaggacg ccgcgccgga ggacaccgcc acggcccgcc
     2521 gccgattcaa ctggatcgcg gcgatcttcc tcctcgcctg cggcgtcagc ggcgtcttcg
     2581 ccgccgtgct ggtcgccttc gtcttccgcg acgcgatcgg cgagccgatc gggctgctgc
     2641 tggcggcctc gctcgtggca ggggtcgtga ccacgtggct gctctaccag ctcggcaagc
     2701 agcgcctgtg cgaccgcgcg atcgtgatgg gcggcgggat cgcgtcggcc gtcatgtgct
     2761 cgatgatgtt cctcgattga gccgtcggcg gcggcgggct ccgggttgac gcacgggcgc
     2821 gctcgccgcg cggcgtggca acatcgggtc atgactcgcg cagactccgc ccgccctcgt
     2881 ccccgtttcc gggc
//
LOCUS       DASVLN010000200         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_63439, whole genome shotgun sequence.
ACCESSION   DASVLN010000200 DASVLN010000000
VERSION     DASVLN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63439"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..875
                     /locus_tag="VF453_04550"
     CDS             207..875
                     /locus_tag="VF453_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7686953.1"
                     /translation="MDALMPVFDHLFHADKYLAAFVAANGAWVYGILFAVIFVETGVV
                     VMPFLPGDSLLFIVGASSATGLLDPWMAVGTMLAAAILGNQTNYTIGRWVGPRVFHWE
                     NSKWFNKRGFDRAHEFYEKYGGFTLIVGRFMPFVRTFAPFVAGVTRMTRSRFTLFDVT
                     GGALWIGSVTAAGWFFGNLPWVRGHIDVIVIATILIPGIAVLFGMLRKKKRKRAAGTT
                     PATR"
     gene            complement(1270..>2107)
                     /locus_tag="VF453_04555"
     CDS             complement(1270..>2107)
                     /locus_tag="VF453_04555"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7686954.1"
                     /translation="APAPPQLAQATAARGAGVDTSAFGPQAPFAPPQPLTPEEKRKVD
                     RLLVIAIDPGHGGEDPGATGPTGLREKDVVLAIATQLRDRLNAKPGVRVLMTRDSDFF
                     VPLGERVRKAERVQADLFVSIHADAFLTPQARGASVFALSEGGATSAAARWMANRENH
                     ADAVGGVNVKSKDSQVMRTLLDMSTAAQIKDSLVLGNEVLSRIGKVGKLHRGQVEQAS
                     FAVLKAPSIPSILVETAFISNPQEEDKLRDPGYRRQLVEALYTGIDRYFAKNPPIRHR
                     DV"
BASE COUNT          280 a          719 c          783 g          325 t
ORIGIN      
        1 gacggcggga gccgcggccg cttcggcggc ggcggcgtgg gaggagggcg tggacatggg
       61 cggtcgcagg tgcgggagca aggcctgccc cggcgcatgg acgggccggg gcggcggcgg
      121 tcgagcgggc tcgacccgcc attgtaggaa gcctggcgcc gcgggagcga atacggcccc
      181 ggaagacccg tcgataatcg ccgcccatgg atgccctgat gcccgtcttc gaccacctgt
      241 tccacgccga caagtacctg gccgccttcg tcgcggccaa cggggcctgg gtctacggca
      301 tcctgttcgc cgtcatcttc gtcgagaccg gggtcgtcgt gatgcccttc ctgccgggcg
      361 actcgctgct gttcatcgtc ggcgcgagct cggccacggg cctgctcgac ccctggatgg
      421 cggtgggcac gatgctcgcg gcggccatcc tcggcaacca gaccaactac acgatcggcc
      481 gctgggtcgg cccgcgcgtc ttccactggg agaactcgaa gtggttcaac aagcgcggct
      541 tcgaccgcgc ccacgagttc tacgagaagt acggcggctt cacgctgatc gtcggccgct
      601 tcatgccctt cgtgcgcacc ttcgcgccgt tcgtggcggg cgtgacgcgg atgacgcgct
      661 cgcgcttcac gctgttcgac gtgaccggcg gcgcgctgtg gatcggcagc gtgaccgcgg
      721 cgggctggtt cttcggcaac ctgccgtggg tgcgcggcca catcgacgtc atcgtgatcg
      781 cgacgatcct gatcccgggg atcgccgtcc tgttcgggat gctgcgcaag aagaagcgaa
      841 agcgcgcggc cggcacgacg cccgcgaccc gctgaatcgc ggccggtacg ccggcccggc
      901 atcgggcgcc ggcgcgaacg gggtttcgaa cgccggcgcc ggtgtttcga acatcggcgc
      961 cgcaccgaaa agatgcgcac gtgcgtttga aaggggcggc gctgccgtcc cgcatccgga
     1021 cgatcgcgtt gcggaccgca actggccggg cgcgcggccg ccgatttgcg gagcgcaagc
     1081 tcggcgccgg catcgcggag ggaaagtcag ccccggaaag gggcggcctt gcattcgaaa
     1141 ataccgtcgt cgatgttttc gaatcggccg tcgatgttcc gcaatgcatt cgcgcgccct
     1201 gcgccgatcg ggcgggccgg cggcaatgga cgcgcgcggc cagcggcgcc gtcgacggcc
     1261 tgcgcgcgtt cagacgtccc ggtgccggat cggcgggttc ttcgcgaagt agcggtcgat
     1321 gccggtgtag agcgcctcga ccagctggcg gcggtacccg gggtcgcgca gcttgtcctc
     1381 ttcctgcggg ttcgagatga aggcggtctc gacgaggatg gacgggatcg acggcgcctt
     1441 gagcaccgcg aacgaggcct gctcgacctg gccgcggtgc agcttgccga ccttgccgat
     1501 gcgcgacagc acctcgttgc cgagcaccag gctgtccttg atctgcgccg cggtgctcat
     1561 gtccagcagg gtgcgcatga cctgcgagtc cttggacttc acgttgacgc cgcccaccgc
     1621 gtcggcgtgg ttctcgcggt tggccatcca gcgggcggcc gcgctcgtgg cgccgccctc
     1681 gctgagcgcg aacaccgacg cgccgcgcgc ctgcggcgtg aggaaggcgt cggcgtggat
     1741 cgagacgaac aggtcggcct gcacgcgctc ggccttgcgc acgcgctcgc ccagcggcac
     1801 gaagaagtcg gagtcgcgcg tcatcagcac tcgcacgccg ggcttggcgt tgaggcggtc
     1861 gcgcagctgg gtggcgatcg cgaggacgac gtccttctcg cgcaggcccg tggggccggt
     1921 cgcgcccgga tcctcgccgc catggccggg atcgatcgcg atcacgagca ggcggtcgac
     1981 cttgcgcttc tcctcgggcg tgagcggctg cggcggcgcg aacggcgcct gcgggccgaa
     2041 cgcgctcgta tcgacgcccg cgccgcgcgc ggcggtcgcc tgggcgagct gcggcggcgc
     2101 cggggcg
//
LOCUS       DASVLN010000201         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_279206, whole genome shotgun sequence.
ACCESSION   DASVLN010000201 DASVLN010000000
VERSION     DASVLN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279206"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VF453_04560"
     CDS             complement(<1..1284)
                     /locus_tag="VF453_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX7686955.1"
                     /translation="MKPGSLSHALGSAMGARIGMAALNYGLFWALSHRLATSTLGGFS
                     LLMNVFFMTALLPLLGLATPLTRRAATERGRLGVETSNALVFATPVAIVLALAIAAVG
                     RFAYGPELRLPFLLLALSMLPTASILVSESTLLGLERMADIARFQFVEALARTVLAIA
                     AVMLGRGLVGVFAVFLALRCAMALAYRLHPLLPRFSRADVSAAVQRRNWREVPVFLGI
                     GLLAAMTARIDIVVLSRMSGLREVGVYAAASRLYEASLMLPTIAALAMMPTLARLFAS
                     DRAYFRDVLVVAMRVSLGAGFVVALGVGALAQPLVGLLYKPEVAAAAPVLRWLMFGAV
                     LMTLDQILSSTMMAAKAQAHDLRALVIALCALGIGLAALVPRLQATGAAIAVTLAICC
                     RVGYRVRWVVRLLELEHLLRELWRVLLAVVAGIAAL"
     gene            complement(1281..>2795)
                     /locus_tag="VF453_04565"
     CDS             complement(1281..>2795)
                     /locus_tag="VF453_04565"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7686956.1"
                     /translation="LAWGLAAVAGALVVAKAAALGESAAAGVGVVGLLGIGAWLAWRR
                     RPKEMLVAGLYFLAPVDVSKALVAPIDRFYSPGLYLTVAEVVLLLLALLWAGRRLFIE
                     RRGLPFTRLDGLALAYFAWLCLRTAGSLQGTLAAASAVSYGLAVLAYYVASHALESPA
                     EIRLALRAAAAGLAAETLWVAAQMATRTPLALPGAKVMPSAGDMLFFGGAGEAFRPTG
                     FFIHPNSLADHMTLLIPPAFALVLLGLRRLDARVWWTALAVLAAATGLLLVTLSRGGW
                     AAAVLGGAWVVWRYARGGLLTRRQLAGMALAGLAGVVALLAVYPEILLRLTAPDERST
                     ESRLILTDQALTIIKAHPWIGVGFGGYNRAAYEYIAPAFANISEEYQQALLELVVHDH
                     YLLIAAELGVPAALFFLALMLRFAGQPGPWGAWRDPTAFALAVGLSGAVVTQVLFLAS
                     DNFYADIRVFMIWLVAGTLQALCLQQRAGLYEPAAGAPPSIPGAVPAAAGRPAE"
BASE COUNT          476 a         1074 c          998 g          247 t
ORIGIN      
        1 cagcgccgcg atgcccgcga ccacggccag cagcacgcgc cagagctcgc gcagcaggtg
       61 ctcgagctcg agcagccgca ccacccagcg cacgcggtag ccgacgcggc agcagatcgc
      121 cagcgtcacc gcgatcgccg cgccggtggc ctgcagccgc ggcaccagcg cggccaggcc
      181 gatcccgagc gcgcacagcg cgatcacgag cgcgcgcagg tcgtgcgcct gcgccttcgc
      241 ggccatcatg gtcgacgaca ggatctggtc gagcgtcatc agcacggcgc cgaacatcag
      301 ccagcgcagc accggcgccg ccgccgcgac ctcgggcttg tacagcaggc cgacgagggg
      361 ctgcgccagc gcgccgacgc cgagcgcgac gacgaagccc gcgcccaggc tcacgcgcat
      421 cgcgaccacc agcacgtcgc ggaagtacgc gcggtcggac gcgaacaggc gcgcgagcgt
      481 cggcatcatc gccagcgcgg cgatcgtcgg cagcatcagc gaggcctcgt acagccgcga
      541 ggccgccgca tacacgccga cctcccgcag ccccgacatc cgcgacagca cgacgatgtc
      601 gatgcgcgcc gtcatcgccg cgagcagccc gatgcccagg aacaccggca cctcgcgcca
      661 gttgcggcgc tgcaccgcgg cgctcacgtc cgcgcgcgag aagcgcggca gcagcggatg
      721 caggcggtag gcgagcgcca tcgcgcagcg cagcgcgagg aaaacggcga acacgccgac
      781 gagcccgcgg cccagcatca cggcggcgat cgcgagcacc gtgcgcgcga gcgcctcgac
      841 gaactggaag cgggcgatgt cggccatgcg ctccagcccg agcagggtgg attccgacac
      901 caggatggag gccgtcggca gcatggacag cgcgagaagc aggaacggca gccgcagctc
      961 cggcccgtac gcgaagcgcc ccaccgcggc gatcgccagg gccagcacga tcgccaccgg
     1021 cgtcgcgaag acgagcgcgt tgctggtctc cacgcccagg cgcccgcgct cggtcgcggc
     1081 gcggcgcgtg agcggcgtcg cgagtcccag cagcggcagc agcgccgtca tgaagaagac
     1141 gttcatcagc agcgagaagc cgccgagcgt cgatgtcgcg aggcggtgcg acaacgccca
     1201 gaacagcccg tagttcagcg ccgccatgcc gatgcgcgca cccatcgcgg agccgagcgc
     1261 gtgcgagagg ctgcccggct tcactcggcc ggcctccctg cggccgcggg tacggcgccg
     1321 ggtatcgacg gcggcgcgcc ggcggcaggc tcgtacaacc ccgcgcgctg ctgcaggcac
     1381 agcgcctgca gggtgcccgc gacgagccag atcatgaaga cgcggatgtc ggcgtagaaa
     1441 ttgtcggagg ccaggaacag cacctgcgtc accaccgcgc ccgacaggcc gaccgccagc
     1501 gcgaacgcgg tcgggtcgcg ccatgcgccc cagggccccg gctggcccgc gaagcgcagc
     1561 atcagcgcga ggaagaacag cgccgccggc acgcccagct ccgcggcgat cagcaggtag
     1621 tggtcgtgca ccaccagctc gagcagcgcc tgctggtact cctcggagat gttggcgaag
     1681 gccggcgcga tgtactcgta ggccgcgcgg ttgtagccgc cgaagccgac gccgatccac
     1741 ggatgcgcct tgatgatcgt gagcgcctgg tcggtgagga tgagccgcga ctcggtcgag
     1801 cgctcgtcgg gcgcggtcag gcgcagcagg atctccgggt acacggccag cagcgccacg
     1861 acgcccgcga gccccgccag cgccatcccc gcgagctggc ggcgcgtcag cagcccgccg
     1921 cgcgcgtagc gccacacgac ccaggcgccg cccagcaccg ccgcggccca gccgccgcgc
     1981 gacagcgtca ccagcagcag gcccgtcgcc gccgcgagga cggccagcgc cgtccaccag
     2041 acccgcgcgt ccagccgccg caggcccagc agcacgagcg cgaacgccgg cgggatgagc
     2101 agcgtcatgt gatcggccag cgagttcggg tggatgaaga agcccgtcgg ccggaacgcc
     2161 tcgccggcgc cgccgaagaa cagcatgtcg ccggccgacg gcatcacctt cgcgccgggc
     2221 agtgcgagcg gcgtgcgcgt cgccatctgc gcggcgaccc acagcgtctc ggctgcgagc
     2281 cccgcggcgg cggcgcgcag cgcgaggcgg atctcggcgg gcgactccag cgcatgcgag
     2341 gcgacgtagt aggccagcac cgccaggccg taggacaccg cgctcgcggc cgccagcgtg
     2401 ccctgcagcg agccggcggt gcgcaggcac agccacgcga agtaggcgag cgcgaggccg
     2461 tccagccgcg tgaacggcag gccgcggcgc tcgatgaaca gccgccggcc ggcccacagc
     2521 agcgcgagca gcagcagcac gacctccgcg accgtgaggt acaggccggg cgagtagaag
     2581 cggtcgatcg gcgcgacgag cgccttggag acgtccaccg gcgcgaggaa atacagcccc
     2641 gccaccagca tctccttggg tcgccgccgc cacgcgagcc aggcgccgat gcccagcagc
     2701 ccgacgacgc ccacgcccgc cgcggccgac tcgcccaggg ccgccgcctt cgcgaccacg
     2761 agcgcccccg ccaccgccgc gaggccccag gcgag
//
LOCUS       DASVLN010000202         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_171658, whole genome shotgun sequence.
ACCESSION   DASVLN010000202 DASVLN010000000
VERSION     DASVLN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171658"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="VF453_04570"
     CDS             <1..121
                     /locus_tag="VF453_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033635647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX7686957.1"
                     /translation="LAAGKLPKVALIACMRKLLTTLNAMARSGLAWDPEFKTA"
     gene            complement(138..1460)
                     /locus_tag="VF453_04575"
     CDS             complement(138..1460)
                     /locus_tag="VF453_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7686958.1"
                     /translation="MSALIIFTLLILLMLTGMPVSIALGLTVMGFFFFLSDVPMASVG
                     MKLFEGLNSFPIMAIPFFILAGNFLTNGGAARRMIVFASKMVGHFYGGLGLAGVLACA
                     LFAAISGSSVATVVAIGSIILPAMVEAGYPKRFSAGVITTSGALGILFPPSINLVIFS
                     IATNGMSATGPDGNPVSTASVGDLFIAGVLPGITLAILLGLTTWWRARKNDYPRMPRA
                     SWGERFKAFLDAFWGLALIIIVIGGIYTGIFTPTEAAAIAAVFAFVISVFVYKDLKFK
                     DVPRVLLNSAAMSSMLLYIITNAVLFSFLLTSEQIPQAMADWMVHQGFGPVVFLLLVN
                     VILLAAGNFMDPSAIVLIMAPILYPVAVKLGVNPVHMGILMAVNMEVGMCHPPIGLNL
                     YVSSGITKMGITELTISVWPWLKTMIAFLILVTYWPPLSLWLPHLLSH"
     gene            complement(1457..2101)
                     /locus_tag="VF453_04580"
     CDS             complement(1457..2101)
                     /locus_tag="VF453_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7686959.1"
                     /translation="MFGRFLDHLEEWLIAFLMGAATLLIFVAVVHRYAAGAHYPPSLQ
                     FIQDFLLQINLGWAQELCIYMFIWMAKFGAAYGVRHGTHVGVDTLVRTLPPAQARWLT
                     IIGLAGGIIFTVVVGTMGAVLVWDDGMKYALFHALGRNVDDLTEGATTPDLELPVWIE
                     YMCIPVASYLMCFRFVQVLVSFWKTGELPHHEVAHVEGLNDVDNYEPTPEGTIK"
BASE COUNT          427 a          709 c          745 g          316 t
ORIGIN      
        1 gctggccgcc ggcaagctgc ccaaggtcgc cctcatcgct tgtatgcgca agctcctgac
       61 cacgctcaac gccatggcca ggagcggcct tgcttgggat cccgagttca aaactgcttg
      121 actcgagaca cggttactca gtgcgacagc aggtggggca gccagagcga caacggcggc
      181 cagtaggtga cgaggatcag gaacgcgatc atcgtcttca gccagggcca gaccgagatc
      241 gtcagctccg tgatgcccat cttcgtgatg cccgaggaca cgtacaggtt caggccgatc
      301 ggcgggtggc acatgccgac ttccatgttc accgccatca ggatgcccat gtgcaccggg
      361 ttcacgccca gcttgaccgc gaccgggtag aggatcggcg ccatgatcag gacgatcgcc
      421 gacggatcca tgaagttgcc ggccgccagc aggatcacgt tgaccagcag caggaacacg
      481 accggcccga agccttggtg caccatccag tcggccatcg cctgcgggat ctgctcggac
      541 gtcagcagga acgagaacag caccgcgttc gtgatgatgt acagcagcat cgagctcatc
      601 gccgccgagt tcagcagcac gcgcggcacg tccttgaact tcaggtcctt gtagacgaag
      661 acggagatca cgaacgcgaa caccgccgcg atcgccgcgg cttccgtcgg cgtgaagatg
      721 cccgtgtaga tgccgccgat gacgatgatg atcagcgcca ggccccagaa cgcgtcgagg
      781 aaggccttga agcgctcgcc ccagctcgcg cgcggcatgc gcgggtagtc gttcttgcgc
      841 gcgcgccacc aggtcgtcag gcccagcagg atcgccagcg tgatcccggg cagcacgccg
      901 gcgatgaaca ggtcgccgac cgaggccgtc gacaccgggt tgccgtccgg tccggtggcg
      961 ctcatcccgt tggtcgcgat cgagaagatc acgaggttga tcgagggcgg gaacaggatg
     1021 cccagcgcac ccgaggtcgt gatgacgccc gcggagaagc gcttcgggta gcccgcctcg
     1081 accatcgccg gcaggatgat cgagccgatc gccacgacgg tggcgaccga cgagcccgag
     1141 atcgcggcga acagggcgca cgcgagcacg cccgccagcc cgaggccgcc gtagaagtgg
     1201 cccaccatct tcgaggcgaa gacgatcatc cgccgcgccg cgccgccgtt ggtcaggaag
     1261 ttgccggcga ggatgaagaa cgggatcgcc atgatgggga agctgttcag gccctcgaac
     1321 agcttcatgc ccacgctggc catcggcacg tcggacagga agaagaagaa gcccatcacc
     1381 gtcaggccga gcgcgatgga caccggcatg ccggtgagca tcagcaggat cagcagggtg
     1441 aagatgatca gcgcgctcat ttgatcgtgc cctccggcgt cggctcgtag ttgtcgacgt
     1501 cgttcagccc ctcgacgtgg gcgacttcgt ggtgcggcag ctcgccggtc ttccagaacg
     1561 aaaccagcac ctgcacgaag cggaagcaca tcaggtacga cgcgaccggg atgcacatgt
     1621 actcgatcca gacgggcagc tcgaggtcgg gggtcgtggc gccttcggtg aggtcgtcga
     1681 cgttgcggcc cagggcatgg aacagcgcgt acttcatgcc gtcgtcccac accagcaccg
     1741 cgcccatcgt cccgacgacc accgtgaaga tgatgccgcc ggccaggccg atgatcgtga
     1801 gccagcgcgc ctgcgcgggc ggcagggtgc gcacgagggt gtcgacgccc acgtgggtgc
     1861 cgtgccggac gccgtaggcg gcgccgaact tggccatcca gatgaacatg tagatgcaca
     1921 gctcctgggc ccagccgagg ttgatctgca gcaggaagtc ctggatgaac tgcagcgacg
     1981 gcggatagtg cgcgccggcg gcgtagcgat ggaccaccgc gacgaagatc agcagcgtcg
     2041 ccgcgcccat gaggaacgcg atgagccact cttcgaggtg gtcgaggaag cgaccgaaca
     2101 tcggacgagg cctttctcgc gcggcgcgcg atgcaggagg gacgcgtgcg cccgccgcgc
     2161 gccggcgtcg ggcgcggcgg catcggacgc ccagtgg
//
LOCUS       DASVLN010000203         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_385734, whole genome shotgun sequence.
ACCESSION   DASVLN010000203 DASVLN010000000
VERSION     DASVLN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385734"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VF453_04585"
     CDS             complement(<1..949)
                     /locus_tag="VF453_04585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7686960.1"
                     /translation="MNGDATARAHWQRVKTLLAQALELEGPAQSAFVDEVCAGDAALR
                     EELVSLLAAARSADEDLDRLPAGLAIASIEAGPHPAWVGRRVGPWRVLSLVASGSAGD
                     VWRAEPVDGRLGQQVAIKAMRICRDAEGVIARLRGELRILTRVAHPNLARVLDGGVTD
                     DGVPYFVMEFVEGEPIDEWCERQRLPLAGRLRLFSEVCRAVHDAHGQHVVHRDLKAGN
                     ILVTHDGVVKLVDFGIPRRFDPATPGPHPVTRHLGAHVYASPEQVRGEDLTAASDIYS
                     LGAVLYRLLTNASPYPGAPAGDDDALVRAIRDADPPPPSR"
     gene            complement(1011..1640)
                     /locus_tag="VF453_04590"
     CDS             complement(1011..1640)
                     /locus_tag="VF453_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7686961.1"
                     /translation="MAAGDTGPPGEVTQLLASWSRGDGDAFARMVPMVYDELRRMAQR
                     HLQRERDGHTLQGTGLVHEVYLRLAEKGPMQWESRGHFFGWASTMMRHILIDHARSRQ
                     AAKRGGGVEVLSLDALREDRGTLPGLSAAVTDEAPDLVAIDRALRKLERLDPQQGRVV
                     ELRYFCGLSVAETAEALAVSPATVKREWVTARAWLLRELSRTDEGGGAP"
     gene            complement(1863..>2388)
                     /locus_tag="VF453_04595"
     CDS             complement(1863..>2388)
                     /locus_tag="VF453_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7686962.1"
                     /translation="EVDPRLALATRSSLPAAWTLAQLEDCSADAPARAPVRRFVQDLA
                     GTGMRGGAFLVLPGEPEGRRHFVSLLARTPDPRLLSGERIGEVLTLGLCLHEYYTRYS
                     GLPDRADPAASLTPVQREILGHVARGESDKRIAHHLSISSHAVDYHMRQLRNRFAVHN
                     RVQLAQASRTLLGA"
BASE COUNT          290 a          885 c          906 g          307 t
ORIGIN      
        1 cgcggctggg cggcggcggg tcggcgtcgc ggatcgcgcg cacgagcgcg tcgtcgtcgc
       61 cggccggcgc gcccgggtag gggctcgcgt tggtcagcag gcggtagagg acggcgccca
      121 gcgagtagat gtcgctcgcc gcggtgagat cttcgccccg cacctgctcc gggctcgcgt
      181 agacgtgcgc gccgaggtgg cgcgtgaccg gatgcggccc gggcgtcgcg gggtcgaagc
      241 gccgcgggat gccgaagtcc accagcttga ccacgccgtc gtgcgtcacg aggatgttgc
      301 cggccttgag gtcgcgatgg acgacgtgct gcccgtgcgc gtcgtgcacc gcgcggcaga
      361 cctcgctgaa gaggcgcagc cgcccggcca gcggcaggcg ctggcgctcg caccattcgt
      421 cgatcggctc gccctcgacg aactccatca cgaagtaggg cacgccgtcg tcggtgacgc
      481 cgccgtccag gacgcgggcg aggttcgggt gcgcgacccg cgtgaggatg cgcagctcgc
      541 cgcgcaggcg cgcgatcacg ccctcggcgt cgcggcagat gcgcatcgcc ttgatggcga
      601 cctgctggcc gaggcggccg tcgaccggct ccgcgcgcca gacgtcgccc gcgctgccgc
      661 tggccaccag cgacagcacg cgccacgggc ccacgcgccg gccgacccag gcaggatgcg
      721 gaccggcctc gatcgaggcg atcgccaggc cggcgggcag gcggtccagg tcctcgtcgg
      781 cgctgcgcgc ggcggccagc agcgagacga gttcctcgcg cagcgccgcg tcgcccgcgc
      841 agacctcgtc gacgaatgcg ctctgcgcgg gcccctcgag ctccagcgcc tgcgcgagca
      901 gcgtcttcac gcgctgccag tgcgcgcgcg cggtcgcgtc gccgttcacc aggcgacctc
      961 cgtgcggcgc gccgcgctgc gggcccccgt cgaagcggcc gggcaagggc tcatggcgcc
     1021 cccccgcctt catcggtgcg gctgagctcg cgcagcagcc acgcgcgcgc ggtgacccat
     1081 tcgcgcttga cggtcgccgg cgagacggcc agcgcctccg cggtctcggc cacgctcagg
     1141 ccgcagaagt agcgcagctc gacgacgcgg ccctgctgcg gatcgaggcg ttcgagcttg
     1201 cgcagcgcac ggtcgatcgc gaccaggtcg ggcgcctcgt ccgtcacggc ggcggacagc
     1261 ccgggcaggg tgccgcggtc ctcgcgcagc gcatccaggc tcagcacctc gacgccgccg
     1321 ccgcgcttgg cggcctggcg cgagcgcgcg tggtcgatga ggatgtggcg catcatcgtc
     1381 gacgcccagc cgaagaagtg gccgcgcgac tcccactgca tcggcccctt ctcggccagc
     1441 cgcaggtaga cctcgtgcac gaggccggtg ccctgcaggg tatggccgtc gcgctcgcgc
     1501 tgcaggtggc gctgggccat gcggcgcagc tcgtcgtaga ccatcggcac catgcgcgcg
     1561 aaggcgtcgc cgtcgccgcg agaccagctc gccagcagct gcgtcacctc gcccggcggc
     1621 cccgtgtcgc ccgccgccat ggccgcagcg acgggggcga tcccgctccc ggtgcgtcga
     1681 cttcgatcgc ctgcctcgtc gtccatgcgg cctgcccgtc ctcgagttga tgcggcgccg
     1741 ccgccgcccg ggtcgggccg cgcggggcgc gtaccgatta tggcggccga gctgtcgatc
     1801 cgcagtaccc cgtccggtat ccacatgtga acgcgaccgg tgtttcgtgc aaagcggccg
     1861 cttcaggcgc ccaggagcgt gcgcgacgcc tgcgccagct gcacgcggtt gtgcacggcg
     1921 aagcggttgc gcagctggcg catgtggtag tccaccgcat gcgaggagat gctcaggtgg
     1981 tgggcgatgc gcttgtcgct ctcgccgcgc gcgacgtggc cgaggatctc gcgctgcacc
     2041 ggcgtcagcg aagccgccgg gtctgcgcgg tcgggcaggc cgctgtagcg cgtgtagtac
     2101 tcgtgcaggc acaggccgag cgtcagcacc tcgccgatgc gctcgcccga gagcagccgc
     2161 gggtcgggcg tgcgcgcgag caggctgacg aagtggcgcc ggccctcggg ctcgcccggg
     2221 agcacgagga acgcgccgcc gcgcatgccg gtgccggcga gatcctgcac gaagcgccgc
     2281 acgggcgcgc gcgcgggcgc gtcggcggag cagtcctcga gctgggccag cgtccacgcg
     2341 gccggcaggc tcgagcgcgt ggccagcgcg aggcgcgggt cgacctcg
//
LOCUS       DASVLN010000204        11114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_63629, whole genome shotgun sequence.
ACCESSION   DASVLN010000204 DASVLN010000000
VERSION     DASVLN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63629"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..581)
                     /locus_tag="VF453_04600"
     CDS             complement(189..581)
                     /locus_tag="VF453_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEX7686963.1"
                     /translation="MQATASGTFRVTMTPRPAGDSPAALGRFLLAKTFEGGLVGTSDG
                     EMLSAGDPAAGSAGYVALEWVDGRLGDRAGRFALQHTGTMDRGAQTLSIVVVPGSGGG
                     ALAGISGRLELAIEDGVHNYTLHYQLPD"
     gene            692..1522
                     /locus_tag="VF453_04605"
     CDS             692..1522
                     /locus_tag="VF453_04605"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX7686964.1"
                     /translation="MTRPVRSQYEDFMRLVRDRGRAKGDRTGTGTTSLFGHQMRFDLS
                     EGFPLVTTKKVHLKSIILELLWFLRGDSNVKWLQERGCTIWDEWAGPDGELGPVYGVQ
                     WRSWPTPEGGHVDQIAQVVHQLRTNPDSRRIIVSAWNVADLPRMALMPCHALFQFYVA
                     PPEAPGARGKLSCQLYQRSADIFLGVPFNIASYALLTHMLAQQCDLDVGDFIWTGGDC
                     HLYDNHAEQVDTQLARTPFPYPTLRIRRRPDSILGYELEDFEVLDYQHHPAIKAPVAV
                     "
     gene            1519..2043
                     /locus_tag="VF453_04610"
     CDS             1519..2043
                     /locus_tag="VF453_04610"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004547470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX7686965.1"
                     /translation="MKIALIAAVSRNGVIGNAGELLWKLPEDMQFFKRATTGSPVVMG
                     RRTWDSIPAKFRPLAARTNIVLTRDREWRADGAIVVHTFEDAIERALESVATEPHASR
                     AWIIGGAEIYALAMPHADELVLTEIDRDFQGDARFPGWTRGDFVEVSRERHRAAAPND
                     FDFSFVTLRRVTPG"
     gene            complement(2015..2623)
                     /locus_tag="VF453_04615"
     CDS             complement(2015..2623)
                     /locus_tag="VF453_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7686966.1"
                     /translation="MPTYDVRPAVRAASAALALLTGLAGAARADTTEAVWFQGTWDHS
                     PDCWELPDQCQSGPPQDVVGPWTGTLDVTLPSWADGTYDASTAHVVMDSFLGGFDSDA
                     WVGIPFWGSITVSGGFVTSIDATWYYPSAPDVTLTFAGLTMDYDQPHEHHYGPTVGGA
                     TLQVPVPLPTVPEPAGWALALAGLAVVGRLRRPVSRASRAAA"
     gene            complement(2801..3616)
                     /locus_tag="VF453_04620"
     CDS             complement(2801..3616)
                     /locus_tag="VF453_04620"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX7686967.1"
                     /translation="MTTAAPLDARFHALHADGLLLLANCWDAGSARLAQEAGARAVAT
                     SSAAVAWSHAWPDGDLLPRELLLQTVRAVAGAVTLPVTVDVEGGFSDDPAEVGELVGA
                     VVEAGAVGINIEDGSAEPALLAAKIAAAREAGKARGVDLFVNARTDVFLRGLAEPGQR
                     VAEVLAREARYRDAGASGIFVPGITDAGEIAAVVAGAKLPLNVMARPGMMPIAQLREL
                     GVRRYSAGSGISELVNGLVAALMRSFVDEGRIEAPAALEAMGYSELNTVMARG"
     gene            3828..4415
                     /locus_tag="VF453_04625"
     CDS             3828..4415
                     /locus_tag="VF453_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX7686968.1"
                     /translation="MTSGFHVHGPHDHEMEHATEHAAAHGDVGGMTGQLAVITAVLAT
                     IGALFSYMGGATQADAALAKNDAAIRKTEASDQWNFYQAKNARQAVAELGLDLVPADK
                     RDQYAKAIAKYKSDKDPIMDKAKELEKEAASFDERSEHLMHLHHRWAAATTFVQISIA
                     LAAIALLTRRRWLEWGTLGVAAIGVATGVLAFLQA"
     gene            4551..5510
                     /locus_tag="VF453_04630"
     CDS             4551..5510
                     /locus_tag="VF453_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7686969.1"
                     /translation="MARARRLTPEFEHDLARDPALGYEAPGPDEFVRCIEHGSPTPLE
                     RWHCHDEYELQLIVGTRGRAFIGDYEGHFEPGHLVLTGPRLPHNWISTDLPPDGLAVR
                     SLVLQFREEPLREGMRAIRELGELRPLLERARRGVEFFGIGEQVRERFQRIRASRGLA
                     RFVEFAGLLCLLQRCEDTRLLSSDPTAGLGDAPGAALASAAIAFIDAHCTEALSVGDV
                     ARRFHMSESRFSRLLRQATDGTFTEFVNRARVHRACEMLMHSERQVAAVCFAAGFNNI
                     ANFNRRFREIKGTTPTDFRAQHRARLGPPLPARRDAPQRRREP"
     gene            5507..6235
                     /locus_tag="VF453_04635"
     CDS             5507..6235
                     /locus_tag="VF453_04635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7686970.1"
                     /translation="MTDRLRDKVAVVTGAASGIGLASARAMLAEGATVVMADRDAAAL
                     EALRAGLGPAMHPLVVDLLDAADCATLVPRALAVAGRIDILHANTGSYVGGDLVDAEP
                     AAIDRMVALNVAVVMKNVQAALGHMIARRTGDVVVTSSLAAHFPTPWEPVYASSKWAI
                     DCFVQTVRRQVFRHGIRVGAISPGPVATGLLADWPADKLREAQASGSLLDPAEVAETI
                     VFMLTRPRGMTIRDIVMMPTQFDL"
     gene            6293..7300
                     /locus_tag="VF453_04640"
     CDS             6293..7300
                     /locus_tag="VF453_04640"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7686971.1"
                     /translation="MKICIYGAGAIGLLIGARLAATGRHEVSAVARGATLAALVEHGF
                     RAETPDGPVGGRALASEHPAVLGVQDVVVVAVKAPALADIAARIAPLLGPHTIVLPAM
                     NGVPWWFCQGLPGAASGLTLSSVDPDGAIARGIPVEHVVGCVVHAASYVGEPGVTHNR
                     MGRGLVIGEPRGGISPRVEQLRAALEAAGFEATASPMIQRDIWYKLWGNMTTNPISAI
                     TGATTDRVMADDLVRQLTTEAMLESGRIGERLGFAIAQTPEERHQITRKLGAFKTSML
                     QDVEAGRAIELDSLVGAVRELGRHVGVATPMIDAIFGLARLFGRVHGLYPEAAPRPSA
                     G"
     gene            7355..8278
                     /locus_tag="VF453_04645"
     CDS             7355..8278
                     /locus_tag="VF453_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7686972.1"
                     /translation="MTAADLPSSSLPTPAAAPRRRLVARHSPLVRVTHWINVLCFSLL
                     LMSGAQIFNAHPRLYWGEYGADFDHAWLAVQSLHDARGWHGALRVGPLVLETTGVLGA
                     SPEGGGLVPRAFPSWLTIPSYQDLATGRRWHFFFAWLFAINGLVYLAAGFARRHFGRD
                     LLPTRAELAPAHLAHEVVTHAKLRFPKGEAARRYNVLQKLAYLGVIFVLLPGMVLTGL
                     TMSPGLDSALHGLLDVFGGRQSARAIHFICASLLVAFVVVHLVMVVASGLWNNLRSMV
                     TGRYAIEEEAMSAERPPEGARAAVRAKEASR"
     gene            8275..9096
                     /locus_tag="VF453_04650"
     CDS             8275..9096
                     /locus_tag="VF453_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX7686973.1"
                     /translation="MSGRAPDLLIRQADAMARFSARRRWLRGLVGGAGALALAGCDRL
                     SQDDRVLATLRGAEKLTMGSQRLILEHQPLAKEYGERDLSPKFRANGSTMPESAEYKR
                     LLQGRFADYRLQVDGLVQRPLALSLAEIKSMKARQQITRHDCVEGWSAIGKWQGVPLG
                     QVLDLAGVKPGARYVIFHCADNLAGTADASGLYYESIDLLDAYHPQTILAYGMNGGDL
                     PVAHGAPLRLRVERQLGYKQAKFVMRVEVADGFSRLHGGHGGFWEDRGYEWYAGI"
     gene            9233..10000
                     /locus_tag="VF453_04655"
     CDS             9233..10000
                     /locus_tag="VF453_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7686974.1"
                     /translation="MKLATWNVNSLSVRLPHLLDWLANTQPDVVVLQETKLVDEKFPH
                     AELREAGWHAEFFGQKTYNGVALLSRVPATDVVRNIPGHADEQARVIAGTVGDVRVVG
                     AYFPNGQAPGSEKFAYKMAWLDALRGWLATELAAHPKLVLMGDFNIAPEDRDVHDPVL
                     WAGQIHCTDEERAHFRGLCGLGLHDAFRLFEQAPKLWSWWDYRNLAFRKNQGLRIDHV
                     LVSEALRAGVTACTIDKAPRKLERPSDHAPVTVEIAG"
     gene            complement(10019..10774)
                     /locus_tag="VF453_04660"
     CDS             complement(10019..10774)
                     /locus_tag="VF453_04660"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7686975.1"
                     /translation="MPLLGILNFAIAIYFGVHAVRTGRNMYWLIILIIAPFLGSLIYF
                     FAEYLPEIRHSAVARKSKRAVSAIVDPNRALREAQQDFERTPTADNRARVAEALLARG
                     DAAAAVEEFRQCASGPYAKDLKFRRGLARAQLAAGQHAQAVQTLESLFADEPQEARGD
                     AALWYAQALAEIDETRALEAFARARQVHETTETLAAYGLYLARLGRDAEARELLERVL
                     HNARVGSATSRELNRDAIDRARAALRTIEARRG"
     gene            complement(10835..>11114)
                     /locus_tag="VF453_04665"
     CDS             complement(10835..>11114)
                     /locus_tag="VF453_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7686976.1"
                     /translation="VPALAPTTFATEPPPAGTPGGGAWLGDLELAARTLLDAGSPDVW
                     EQLSRQFEGQLIRTALALTRGRRIEAAQKLGIGRNTITRKIQELGLDD"
BASE COUNT         1540 a         4117 c         3954 g         1503 t
ORIGIN      
        1 cccgcgcagg cgcccgcccg ggcggccgcg gcgcccgcgt gcgccgtccc ccgtcccggc
       61 cgtcgcaccg gaccgcctgg cctcgttcac gcttgcttca tcgttgttcg tccccgacgc
      121 gcgagccctc cggcgcgcat ccgcctgccg gcccatcgtg cgtcgggcca cccgggcggt
      181 caaccgggtc agtcgggcag ctggtagtgc agcgtgtagt tgtgcacgcc gtcctcgatc
      241 gccagctcca gccgtccgct gatgccggcc agcgccccgc cgcccgagcc cggcaccacg
      301 acgatggaca gtgtctgcgc gccgcggtcc atcgtgccgg tgtgctgcag cgcgaagcgg
      361 ccggcccggt cgccgaggcg gccgtccacc cattcgagcg cgacgtagcc cgccgagccc
      421 gcggccgggt cgccggccga cagcatctcg ccgtcgctcg tgcccacgag gccgccctcg
      481 aaggtcttgg cgagcaggaa gcggccgagc gccgcggggc tgtcgccggc cggacgcggg
      541 gtcatggtga cgcggaaggt gccggaggcg gtggcttgca tggggggctc ccgtggatgg
      601 cgtgcgcgcg agcatacgtc ctcacgcccg gcatcgccgg cgaagaccgc gtgcgcgggg
      661 gaggatggcc aagtccccct agaatccgcc gatgacccgc cccgtccgtt cgcagtacga
      721 agatttcatg cgcctcgtgc gcgaccgcgg ccgcgccaag ggcgaccgca ccgggacggg
      781 cacgaccagc ctcttcggcc accagatgcg cttcgacctg tcggagggct tcccgctggt
      841 gacgaccaag aaggtgcacc tgaagtccat catcctcgag ctgctgtggt tcctgcgcgg
      901 cgactcgaac gtgaagtggc tgcaggagcg cggctgcacg atctgggacg agtgggccgg
      961 ccccgacggc gagctcggcc cggtctacgg cgtgcagtgg cgctcgtggc cgacgcccga
     1021 gggcggccac gtcgaccaga tcgcccaggt cgtccaccag ctgcgcacca atcccgactc
     1081 ccgccgcatc atcgtcagcg cctggaacgt cgccgacctg cccaggatgg cgctgatgcc
     1141 ctgccacgcg ctcttccagt tctacgtcgc gccacccgag gcgcccggcg cccgcggcaa
     1201 gctgtcgtgc cagctctacc agcgcagcgc cgacatcttc ctgggcgtcc cgttcaacat
     1261 cgcgagctac gcgctgctca cgcacatgct cgcgcagcag tgcgacctcg acgtcggcga
     1321 cttcatctgg accggcggcg actgccacct ctacgacaac cacgccgagc aggtcgacac
     1381 ccagctcgcg cgcacgccgt tcccctaccc gacgctgcgc atccggcgcc ggcccgactc
     1441 gatcctcggc tacgagctgg aggacttcga ggtgctcgac taccagcacc acccggcgat
     1501 caaggcgccg gtcgcggtat gaagatcgcg ctgatcgccg cggtctcgcg caacggcgtc
     1561 atcggcaacg ccggcgagct gctgtggaag ctgcccgagg acatgcagtt cttcaagcgc
     1621 gcgaccacgg gctcgccggt cgtcatgggc cgtcgcacct gggattcgat cccggcgaag
     1681 ttccggcccc tcgcggcgcg caccaacatc gtcctcacgc gcgaccgcga gtggcgggcg
     1741 gacggggcga tcgtcgtcca caccttcgag gacgcgatcg agcgcgcgct ggaatccgtc
     1801 gccaccgagc cgcacgcctc gcgcgcctgg atcatcggcg gcgccgagat ctacgcgctc
     1861 gcgatgccgc acgccgacga gctcgtgctc accgagatcg accgcgactt ccagggcgat
     1921 gcccgcttcc ccggctggac gcgcggcgat ttcgtcgagg tctcgcgcga gcggcaccgt
     1981 gccgccgcgc ccaacgactt cgacttctcg ttcgtcacgc tgcggcgcgt gacgcccggc
     2041 tgacgggccg gcgcaaccgg ccgacgacgg ccaggccggc cagggccagc gcccagcccg
     2101 cgggctccgg aaccgtcggc aaaggcacgg ggacctgcag cgtcgcgccg ccgacggtcg
     2161 ggccgtagtg gtgctcgtgc ggctggtcgt agtccatggt caggccggcg aaggtcagcg
     2221 tcacgtccgg cgcgctcggg tagtaccagg tcgcgtcgat cgaggtcacg aagccgccgg
     2281 agacggtgat cgagccccag aacgggatgc ccacccaggc gtcggagtcg aagccgccca
     2341 ggaacgaatc catcacgacg tgcgcggtcg acgcatcgta ggtgccgtcc gcccaggacg
     2401 gcagcgtgac gtccagggtg cccgtccacg ggccgaccac gtcctgcggc gggccggact
     2461 ggcattgatc gggcagctcc cagcaatcgg gcgagtgatc ccaggtgccc tggaaccaca
     2521 ccgcctcggt ggtgtcggcg cgcgcggcgc cggccaggcc ggtgagcagc gcgagggctg
     2581 cggatgccgc gcgaaccgcg ggccggacgt cgtaggtcgg catgtttcct cccaaggatg
     2641 aagacttgac gaccggcgac tctagcggca acggcccgtc gcggcgcccc cctagaagcg
     2701 acctgcggcc gggcacgccg atgaaaacgc ggagcttgcg tgaaggtgtc cccgacggcc
     2761 ggggaagggt gcccgtgagg ccgatccggg ccgtgcgcct tcagccgcgc gccatcaccg
     2821 tgttcagctc ggagtacccc atcgcctcga gcgcggcggg cgcctcgatg cggccctcgt
     2881 cgacgaagct gcgcatcagc gccgcgacga ggccgttgac cagctcggag atgccggagc
     2941 ccgcgctgta gcggcgcacg cccagctcgc gcagctgcgc gatcggcatc atgccgggac
     3001 gagccatcac gttcagcggc agcttggcgc cggcgacgac ggccgcgatc tcgccggcgt
     3061 cggtgatgcc gggcacgaag atgccgctcg cgccggcgtc gcggtagcgc gcctcgcgcg
     3121 cgagcacctc ggccacgcgc tggccgggct cggccaggcc gcgcaggaac acgtcggtgc
     3181 gggcgttgac gaacaggtcg acgccacgcg ccttgccggc ctcgcgcgcg gccgcgatct
     3241 tggcggcgag cagcgcgggc tcggcgctgc cgtcctcgat gttgatgccg acggcgccgg
     3301 cctcgaccac cgcgccgacc agctcgccca cctcggccgg gtcgtcggag aagccgcctt
     3361 cgacgtccac cgtcaccggc agcgtgaccg cgcccgcgac cgcgcgcacg gtctgcagca
     3421 gcagctcgcg cggcagcagg tcaccgtcgg gccaggcgtg cgaccaggcg acggcggcgc
     3481 tgctggtggc caccgcgcgc gcgccggcct cctgggcgag gcgggcgctg ccggcgtccc
     3541 agcagttggc cagcagcagc aggccgtcgg catgcagggc gtggaagcgg gcgtcgagcg
     3601 gtgcggcggt cgtcatggcg tcctttcggg gtcggcggtg gcgagggggg tcggcgcgcg
     3661 atgttcgcac aggcgcacgc gccgttgccg gccggatgcg gacatgcccc tgcccgacgg
     3721 ccggcaatcg ggccgcccgc gacgggcccc cgtcaggccc gttcgctacc atcgcggcca
     3781 tcgacgcccg cgcgcgtccc gaaggcaacg catcggagag ttcccgcatg acctcaggct
     3841 tccacgtgca cgggccgcac gaccacgaga tggagcacgc gaccgagcac gccgcagccc
     3901 acggcgacgt cggcgggatg accggccagc tcgccgtcat caccgcggtg ctggcgacga
     3961 tcggcgccct gttctcgtac atgggcggcg cgacccaggc cgacgccgcg ctggccaaga
     4021 acgatgccgc gatccgcaag accgaggcat ccgaccagtg gaacttctac caggcgaaga
     4081 acgcgcgcca ggcggtcgcg gagctgggcc tcgacctcgt gcctgccgac aagcgcgacc
     4141 agtacgccaa ggcgatcgcc aagtacaagt cggacaagga tccgatcatg gacaaggcca
     4201 aggagctcga gaaggaggcc gcgtccttcg acgagcgcag cgagcacctg atgcacctgc
     4261 accaccgctg ggccgcggcg accaccttcg tccagatctc gatcgcgctg gcggcgatcg
     4321 ccctgctgac ccgccggcgc tggctcgagt ggggcacgct gggcgtggcc gcgatcggcg
     4381 tggcgaccgg cgtgctggcg ttcctccagg cttgagaacc cgcgcaaaag tatcggtatc
     4441 ggcctgccgc gtatcggcag cgcgggcatc cgggtctatc gttgcgccat cgacggccgc
     4501 gcggtcaccg cgccgccggc ggggtcgacc accaccagga gcatcgcagc atggccaggg
     4561 cccgcaggct cacccccgag ttcgagcatg acctcgcgcg cgaccccgcg ctcggctacg
     4621 aggcgcccgg ccccgacgaa ttcgtgcgct gcatcgagca cggcagcccg acgccgctgg
     4681 aacgctggca ctgccacgac gagtacgagc tgcagctgat cgtcggcacg cgcggccgcg
     4741 cgttcatcgg cgactacgag ggccatttcg agccgggcca cctcgtgctc accggcccgc
     4801 gcctgccgca caactggatc tccaccgacc tgccgcccga cggcctggcc gtgcgcagcc
     4861 tggtgctgca gttccgcgag gagccgctgc gcgagggcat gcgtgcgatc cgcgagctcg
     4921 gggagctgcg gccgctgctg gagcgtgcgc ggcgcggcgt cgagttcttc ggcatcggcg
     4981 agcaggtgcg cgaacgcttc cagcgcatcc gggcgagccg cggcctcgcg cgcttcgtcg
     5041 agttcgccgg cctgctgtgc ctgctgcagc gctgcgagga cacgaggctg ctgtcctccg
     5101 acccgacggc cggcctgggc gacgccccgg gcgcggcgct cgcgtccgcg gcgatcgcct
     5161 tcatcgacgc gcactgcacc gaggcgctgt cggtcggcga cgtcgcgcgc cgcttccaca
     5221 tgagcgagag ccgcttctcg cgcctcctgc gccaggccac cgacggcacc ttcaccgagt
     5281 tcgtcaaccg cgcgcgcgtg catcgcgcct gcgagatgct gatgcacagc gagcgccagg
     5341 tggcggccgt ctgcttcgcc gccggattca acaacatcgc caacttcaac cggcgcttcc
     5401 gcgagatcaa gggcaccacc ccgaccgact tccgcgcgca gcaccgggcc cgcctcgggc
     5461 cgcccctgcc ggcgcggcgc gacgcaccac agcggagacg agaaccatga cagacagact
     5521 gcgggacaag gtggccgtcg tgaccggcgc ggcctcggga atcggcctgg ccagcgcgcg
     5581 cgcgatgctg gccgagggcg cgaccgtcgt gatggccgac cgcgacgccg cggcgctgga
     5641 ggcgctgcgc gccgggctcg ggccggcgat gcatccgctc gtcgtcgacc tgctcgacgc
     5701 cgcggactgc gccacgctcg tgccgcgcgc gctggcggtg gccgggcgca tcgacatcct
     5761 gcacgccaac accggcagct acgtgggcgg cgacctcgtg gacgccgagc ccgccgcgat
     5821 cgaccgcatg gtggcgctca acgtcgccgt cgtgatgaag aacgtgcagg ccgccctcgg
     5881 ccacatgatc gcgcgccgca ccggcgacgt cgtcgtgacg agctcgctgg cggcgcactt
     5941 cccgacgcct tgggagccgg tctacgcgtc gtcgaagtgg gcgatcgact gcttcgtgca
     6001 gaccgtgcgg cggcaggtgt tccggcacgg catccgggtc ggcgcgatct cgccgggccc
     6061 ggtcgccacc ggcctgctcg cggactggcc ggccgacaag ctgcgcgagg cgcaggcctc
     6121 gggcagcctg ctcgatccgg ccgaggtcgc cgagaccatc gtcttcatgc tgacgcggcc
     6181 tcgcggcatg acgatccgcg acatcgtcat gatgccgacc cagttcgacc tttgaggcgc
     6241 gggcctgcgc gactgcgcca gaatgcacgc tccacggcgg catccggaca ccatgaagat
     6301 ctgcatctac ggcgcgggcg cgatcggcct gctcatcggc gcgcgcctcg cggccacggg
     6361 ccggcacgag gtgagcgcgg tcgcgcgcgg agcgacgctc gcggcgctcg tcgagcacgg
     6421 ctttcgcgcc gagacgcccg acggcccggt cggcggccgt gcgctcgcga gcgagcaccc
     6481 cgccgtgctg ggcgtgcagg acgtcgtcgt cgtcgccgtg aaggcgccgg cgctggccga
     6541 catcgccgcg cgcatcgcgc cgctgctggg cccgcacacg atcgtgctgc cggcgatgaa
     6601 cggcgtgcca tggtggttct gccaggggct gccgggcgcg gcctccgggc tcacgctgtc
     6661 cagcgtcgac ccggacggcg cgatcgcgcg cggcatcccg gtcgagcacg tcgtcggctg
     6721 cgtcgtccac gccgccagct acgtgggcga gccgggcgtc acgcacaaca ggatgggccg
     6781 cgggctcgtg atcggcgagc cgcgcggcgg aatctcgccg cgcgtggagc agctgcgggc
     6841 cgcgctcgag gcggccggct tcgaggccac cgcctcgccc atgatccagc gcgacatctg
     6901 gtacaagctc tggggcaaca tgaccaccaa cccgatcagc gcgatcaccg gcgccacgac
     6961 cgaccgcgtg atggccgacg acctggtgcg ccagctcacg accgaggcga tgctggaatc
     7021 gggccgcatc ggagagcgcc tgggcttcgc gatcgcgcag acgcccgagg aacgccacca
     7081 gatcacgcgc aagctgggcg cgttcaagac ctcgatgctg caggacgtcg aggcgggccg
     7141 cgcgatcgag ctggacagcc tggtgggcgc ggtgcgcgag ctcggccggc acgtgggggt
     7201 cgccacgccg atgatcgacg ccatcttcgg gctcgcacgc ctgttcggcc gcgtgcacgg
     7261 gctctatccc gaggccgcgc cgcggccgtc ggcgggctga atgtaaggtt ccggcgtgcc
     7321 gcgcgaccaa cggcggagta ccctcgctgc tgccatgacc gccgccgacc tgccctcctc
     7381 ctcgctgccg acgccggccg ccgcgccgcg gcgccggctc gtcgcgcgcc actcgccact
     7441 cgtgcgcgtc acgcactgga tcaacgtcct gtgcttctcg ctgctgctga tgtcgggcgc
     7501 gcagatcttc aacgcccatc cgcgcctgta ctggggcgag tacggtgccg acttcgacca
     7561 cgcctggctc gcggtgcagt cgctgcacga cgcgcgcggc tggcacggcg cgctgcgggt
     7621 cgggccgctg gtgctcgaga cgaccggcgt gctgggtgcc tcgccggaag gcggtggcct
     7681 ggtgccgcgc gcgttcccct cgtggctgac gatcccgtcc taccaggatc tcgcgaccgg
     7741 ccgccgctgg cacttcttct tcgcgtggct gttcgcgatc aacgggctgg tctacctggc
     7801 cgccgggttc gcgcgccgcc acttcggacg cgacctgctg cccacgcgcg ccgagctcgc
     7861 gccggcgcac ctggcccacg aggtcgtcac gcacgcgaag ctgcggttcc ccaagggcga
     7921 ggccgcccgc cgctacaacg tgctgcagaa gctcgcctac ctcggcgtga tcttcgtgct
     7981 gctaccgggc atggtgctga cgggactgac gatgtcgccc ggcctcgact ccgcgctcca
     8041 cgggctgctc gacgtcttcg gcggccgcca gtcggcgcgt gcgatccact tcatctgcgc
     8101 gtcgctgctg gtcgccttcg tggtggtgca cctcgtgatg gtcgtggcct cggggctgtg
     8161 gaacaacctg cgctcgatgg tgaccgggcg ctacgcgatc gaggaggagg cgatgagcgc
     8221 cgaacggccg cccgaaggcg ctcgcgccgc tgtccgcgcg aaggaggctt cgcgatgagc
     8281 ggccgcgctc ccgacctcct gatccgccag gcggacgcca tggcccgctt cagcgcccgt
     8341 cgccgctggc tgcgcgggct ggtcggcggc gcgggcgcgc tggcgctggc cggctgcgac
     8401 cgcctgtcgc aggacgaccg cgtcctggcc accctgcgcg gtgccgagaa gctgacgatg
     8461 ggcagccagc ggctgatcct cgagcaccag ccgctcgcca aggagtacgg cgagcgcgac
     8521 ctctcgccga agttccgcgc caacggctcc acgatgcccg agagcgccga gtacaagcgc
     8581 ctgctgcagg gccgcttcgc cgactaccgg ctgcaggtcg acggtctcgt gcagcggccg
     8641 ctcgcgctgt cgctggccga gatcaagtcg atgaaggcgc gccagcagat cacgcgccac
     8701 gactgcgtcg agggctggag cgcgatcggc aagtggcagg gcgtgccgct cgggcaggtg
     8761 ctcgacctcg ccggcgtgaa gccgggtgcg cgctacgtga tcttccactg cgcggacaac
     8821 ctggccggca ccgccgacgc gagcggtctt tactacgaga gcatcgacct gctggatgcc
     8881 taccacccgc agaccatcct cgcctacggg atgaacggcg gcgacctgcc cgtcgcccac
     8941 ggcgccccgc tgcgcctgcg cgtcgagcgc cagctcggct acaagcaggc caagttcgtg
     9001 atgcgcgtcg aggtcgcgga cggtttctcg cgcctgcacg ggggccacgg cggcttctgg
     9061 gaagaccgcg gctacgagtg gtacgcgggc atctgaagcg gcatgcgcgg atccggcgcg
     9121 ccggcgcacc ccgcgcgggc gcgatgcgcg tcgatggtgc ggcgaacgtg caccgcgaag
     9181 cggcccgcgc gggcgcacaa tcgcggctcg accatcacgc gccgacccgc ccatgaagct
     9241 cgccacctgg aacgtcaact ccctgtccgt gcgcctgccg cacctgctcg actggctggc
     9301 gaacacgcag cccgacgtcg tcgtgctgca agagaccaag ctcgtcgacg agaaattccc
     9361 gcacgcggag ctgagggagg ccggctggca tgccgagttc ttcggccaga agacctacaa
     9421 cggcgtcgcc ctgctctcgc gcgtgccggc caccgacgtc gtgcgcaaca tccccggcca
     9481 cgccgacgag caggcgcgcg tgatcgcggg cacggtgggc gacgtgcgcg tcgtcggcgc
     9541 ctacttcccc aacggccagg cgccgggcag cgagaagttc gcctacaaga tggcctggct
     9601 cgacgccctg cgaggctggc tcgcgaccga gctcgccgcg catccgaagc tcgtgctgat
     9661 gggcgacttc aacatcgcgc cggaagatcg cgacgtccac gatcccgtgc tgtgggccgg
     9721 ccagatccac tgcaccgacg aggagcgcgc gcatttccgc ggcctgtgcg gcctcggcct
     9781 gcacgacgcg ttccgcctgt tcgagcaggc gcccaagctg tggagctggt gggactaccg
     9841 caacctcgcg ttccgcaaga accagggcct gcgcatcgac cacgtcctcg tgagcgaggc
     9901 gctgcgtgcc ggggtcaccg cctgcacgat cgacaaggcg ccgcgcaagc tcgagcgccc
     9961 gagcgaccac gcgccggtga cggtggagat cgccggctga gggccggccg cgcgacgttc
    10021 agccgcggcg cgcctcgatg gtccgcagcg ccgcgcgcgc gcggtcgatc gcgtcgcgat
    10081 tgagctcgcg cgaggtcgcg ctgccgacgc gggcgttgtg cagcacgcgt tccagcaact
    10141 cgcgcgcctc ggcgtcgcgg ccgaggcggg cgaggtacag gccataggcg gccagcgtct
    10201 cggtcgtctc gtgcacctgg cgggcgcgtg cgaaggcctc gagcgcccgc gtctcgtcga
    10261 tctcggcaag cgcttgcgcg taccacaggg ccgcgtcgcc gcgtgcctcc tgcggctcgt
    10321 cggcgaacag cgactcgagc gtctgcaccg cctgcgcatg ctgccccgcg gcgagctgcg
    10381 cgcgcgcgag gccgcgccgg aacttgagat ccttcgcgta ggggccgctc gcgcactggc
    10441 ggaactcctc gaccgcggcc gccgcgtcgc cgcgcgcgag cagcgcctcg gccacgcggg
    10501 cacggttgtc ggcggtgggc gtgcgctcga agtcctgctg cgcctcgcgc agcgcacggt
    10561 tcggatcgac gatcgccgac accgcccgct tcgacttgcg cgccacggcg gagtggcgga
    10621 tctcgggcag gtactcggcg aagaagtaga tcaggctgcc caggaacggc gcgatgatca
    10681 ggatgatcag ccagtacatg ttgcggcccg tgcggaccgc gtggacgccg aaatagatgg
    10741 cgatcgcgaa gttcagaatg cccagcagcg gcatcgttcc ccccagttgt cggaacccgc
    10801 gattttaagg cgtgcgaagg aaggcggctg cccgtcagtc gtcgagcccg agctcctgga
    10861 tcttgcgcgt gatcgtgttg cgcccgatgc ccagcttctg cgccgcctcg atgcgccgcc
    10921 cgcgcgtgag cgccagcgca gtgcggatca gctggccctc gaactggcgg ctgagctgct
    10981 cccagacgtc gggcgagccg gcgtccagca gcgtgcgcgc cgcgagctcg agatcgccca
    11041 gccacgcacc gccacccggg gtgccggccg gcggcggctc ggtcgcgaac gtcgtgggcg
    11101 cgagcgccgg gacg
//
LOCUS       DASVLN010000205         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_63667, whole genome shotgun sequence.
ACCESSION   DASVLN010000205 DASVLN010000000
VERSION     DASVLN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63667"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1536)
                     /locus_tag="VF453_04670"
     CDS             complement(61..1536)
                     /locus_tag="VF453_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7686977.1"
                     /translation="MQLQVLTVLAHPDAAPFLKRQVLDGRLREALAAAEDALDARHPE
                     AGANDESLCVLAQLCGDLMLAQDRAEEAEETYRLAVKLAGGARRGTVRVVSCRSTGYL
                     SLYRQRHGTALACFARLAADEAATTAQRVEAHCAMAVAQQALGQIAAAAQALDKAGEL
                     ACSAGDPDLAMLASLMRVDFAVARALRTHDALQDHVFWRPATRARVPERDDLQPLATL
                     DICLAAHGHHGLVAQRLRHLRDLLRASYGEPGLQAALLDHLAQLRACGLSTLEQQARM
                     DTALVALSQRSTELARSVLEPVLARDAAGSRRWSVELAFCQAKLSQLAGRVEEAMQYY
                     QRYAFESMQCVRAGAAVPVERAAAPDAAVPVEARDDIEMRLPAKYRRAYRYLQSHLDC
                     PGLTVREIADDMGVTERALQSAFKTYLGMTPGEIVQRCRVERIRADLLREGAPGTSVI
                     ETAARWGIRNRSTLIASYRKYFRETPTQTLARRGLQVVAAA"
BASE COUNT          227 a          659 c          696 g          248 t
ORIGIN      
        1 gccgcgcagg cgagcgcgat cagcccgcga tgcagcgccg cgacgcccgg cacgtccgcc
       61 tcacgccgcg gcgacgacct gcaggccgcg ccgcgccagc gtctgggtcg gcgtctcgcg
      121 gaagtacttc cggtacgagg cgatcagcgt cgagcggttg cggatccccc agcgggccgc
      181 ggtctcgatg acgctggtgc ccggggcgcc ctcgcgcagc aggtcggcgc ggatgcgttc
      241 gacccggcag cgctgcacga tctcgcccgg cgtcatgccc aggtaggtct tgaaggcgga
      301 ctgcagcgcg cgctccgtga cgcccatgtc gtcggcgatc tcgcgcacgg tcaggccggg
      361 gcagtccagg tgcgactgca ggtagcgata cgcgcggcgg tatttcgccg gaaggcgcat
      421 ctcgatgtcg tccctggcct cgaccggcac cgccgcgtcg ggtgccgcgg cgcgttcgac
      481 gggcaccgcg gcgccggcgc gcacgcactg catcgactcg aaggcgtagc gctggtagta
      541 ctgcatggcc tcctcgacac ggccggcgag ctggctcagc ttggcctggc agaacgcgag
      601 ctccacgctc cagcggcgcg agcctgcggc gtcgcgggcg agcaccggct cgagcacgga
      661 gcgggcgagc tcggtgctgc gctgcgacag cgcgaccagc gcggtgtcca tgcgcgcctg
      721 ctgctcgagc gtcgacagcc cgcacgcgcg cagctgcgcg aggtggtcga gcagcgccgc
      781 ctgcaggccg ggctcgccgt agctcgcgcg cagcaggtcg cgcaggtggc gcaggcgctg
      841 cgcgaccagc ccgtggtggc cgtgcgccgc gagacagatg tccagcgtgg cgagcggctg
      901 caggtcgtcg cgctcgggca cgcgggcgcg tgtcgccggt cgccagaaca cgtggtcctg
      961 aagcgcgtcg tgcgtgcgca gtgcacgggc gaccgcgaag tcgacgcgca tgaggctggc
     1021 cagcatcgcg agatccgggt cgccggcgga gcacgcgagc tcgcccgcct tgtccagggc
     1081 ctgcgcggcg gccgcgatct ggcccagcgc ctgctgcgcc accgccatcg cgcaatgcgc
     1141 ctcgacgcgc tgggccgtcg tggcggcctc gtcggcggcg aggcgggcga agcacgccag
     1201 cgccgtgccg tgccgctggc gatacaggct gaggtatccg gtgctgcggc acgacaccac
     1261 ccgcacggtg ccgcgccgcg ccccgccggc gagcttcacg gcgaggcggt aggtctcctc
     1321 ggcctcctcc gcgcggtcct gggcgagcat caggtcgccg cacaactgcg cgaggacgca
     1381 gagggattcg tcgttcgcgc cggcctcggg atggcgcgcg tccagcgcgt cttcggcggc
     1441 ggcgagcgcc tcgcgcagcc ggccgtccag cacctggcgc ttcaggaagg gggccgcgtc
     1501 gggatgcgcg agcacggtga ggacttggag ctgcatgggg tctctctcgg tctcgggctt
     1561 cgacatgaag ccgctgtccg atcgatgcta cgaacgcagt cccgagcaga ttctcaacaa
     1621 atctctgtcg cgtcccgtaa cgtctcggca tcgtggcggc gcgcacgccc gggcgcttgc
     1681 cagggttcga cggctcggca gccgcgctcg ccggcggctt gccggcgccg gcacgtgagt
     1741 cgggcccggc atgactgcgg ctttcgcgtc ggaggttcgc gttggcgacc gcgcgcgacg
     1801 ccgcggcgat gccggatccg cgcaaggccc
//
LOCUS       DASVLN010000206         6927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_279417, whole genome shotgun sequence.
ACCESSION   DASVLN010000206 DASVLN010000000
VERSION     DASVLN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279417"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /gene="tagF"
                     /locus_tag="VF453_04675"
     CDS             complement(<1..303)
                     /gene="tagF"
                     /locus_tag="VF453_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HEX7686978.1"
                     /translation="MATCGFYGKLPSEGDFVSRRLPWEFTSAWDDWLQQGMQASRGAL
                     GERWLELYLSAPVWRFQLAPGVCGPLAWRGLFFASVDRVGRYFPLTLAFAGGPGRAG"
     gene            complement(364..3909)
                     /gene="tssM"
                     /locus_tag="VF453_04680"
     CDS             complement(364..3909)
                     /gene="tssM"
                     /locus_tag="VF453_04680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEX7686979.1"
                     /translation="MWAKLKAFFSNRWVLAVIGLLVVSLLIWFVGDAIAFYDHRPLGS
                     AGARLGLILFIILCFAVWEGVKRWRAWRANKQLLSAIGEGASSNSALSQREVAELKKR
                     FDEAMDTLRKARFDDKSGGGRNYLYQLPWYMFIGAPGSGKTTALVNSGLRFPLEGKMG
                     KDAVKGIGGTRNCDWWFTDEAVLLDTAGRYTTQSSNREVDAAAWTGFLTLLKKFRPAQ
                     PLNGAIVTVSLSDLFTQSAAQRDEYARSVRARIQELYSTLGVRFPIYVMVTKTDLLAG
                     FSEFFGDLGREARAQVWGTTFDFPMPVGAALGETFGREFDALVGRLNARLTTRMEEER
                     DPQRRATLFAFPQQFAALREMLRAFLDEAFLSSSFNEEPMVRGVYFTSGTQEGSPFDR
                     VLGNISRAYGLERQVLAPAAASGRSYFLTHLLRSVVFAESDISGRSAVIERRRSRVAV
                     VAYSLLALFAVLLIAGWTISFVRNEALISEVSAAQQDVSKQVQATPPVTVGDVMTVLP
                     LLDAARAIPTGYADRDAPTPWSRSFGLSQAGKLGTQANAAYLNLLRDVFMPRIALRLE
                     QQIRDAQSPEVRYEALKTYIELYDPTHLKAQDVEAWIGADWDRTLPRDISQQQRGNLR
                     DHLDAALAQTPLEMALPRDQALIDEVRKQLAASSLAERAYSRLKRLGAGDGVKDFVVS
                     DAGGPQAALVFTRASGTPLTQGIPGLYTVAGFDKGFRRQALPMVKQLGDEQAWVLGPA
                     AGSADARIATTYTQVEQLYLNDYIKVWDDLLGDLRLVPSTSLQESIQTITLLSASDSP
                     MKLLLNGVARETKLTGNNTRSAEAAAAGAMDKVTGAMRSTVDKLMGSKPADTGDAQAK
                     PEAIVDQHFEQLQRVVAGPAGQPVPLDATLGVLKEYEVQLRATDEAIKRGATPTSDTL
                     IIARIKSEADRMPPPISNLLNGLISRSTAQSASSGQAAVKAAAAGEVGSFCKQAVEGR
                     YPFTRGVPKEVPLGDFARLFAPNGVIDGFVKTKLVGMVDMNGPVWKPIQIADGVNSLD
                     PATIVNFQRASVIRDAFFPGGSANPSASFDLLLQRADDGVSDVQVSVDGQVTHLAPGG
                     NAGMRLSWPSMSPTSQIKLTLVAGGKPSTNPLIFDGPWALFRFADAGKVEGGTPERQM
                     ISYQVNDKHVWFELRSGSVKNPLRLPELAQFRCPG"
     gene            complement(3925..5268)
                     /gene="icmH"
                     /locus_tag="VF453_04685"
     CDS             complement(3925..5268)
                     /gene="icmH"
                     /locus_tag="VF453_04685"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEX7686980.1"
                     /translation="MSQPPFPNLPGLPPNKPQPDSESTIMIPKPGGRRPAGPAPTAGQ
                     AVSLDTAQVLSDVEESAAPVGLNPLVANASVLLNAVPTIRRTLNHADPAGLRAYLLRA
                     ITDFEARARAAGSSPEHVLIARYALCTMIDEAVANMPWGASPEWVQQSLLVTLHREGF
                     GGEKFFQLLDKAMEDPRRNLELLELMYVCLALGFEGRYSVMENGRAQLDAVRDRLHDV
                     IRRERGEFERDLSGKWKGQQRQAKPLVRRLSAWIVLAAVALLLFVLYLVFALVLGSKS
                     DPVFSALGSIHSDVGDLKRPPPPAPPPAAPRLKPLLAAEIAQGLLDVDEDATSSHVVI
                     HGDKFFDPGSAEIRPELVPVITRVAQAINQVPGVVTVSGHTDNQPTKRSLRFPSNYEL
                     SLERARGVAALLAQTVQKHTIKAEGLADSKPLVPNTTPAGRAQNRRVEITLRTGT"
     gene            complement(5327..6664)
                     /gene="tssK"
                     /locus_tag="VF453_04690"
     CDS             complement(5327..6664)
                     /gene="tssK"
                     /locus_tag="VF453_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEX7686981.1"
                     /translation="MSRKRVIWSEGLFLRPQHFQQLERSVEMLVDARTHGVFAPAWGL
                     GTLRLDDEALKIGRLAVKAARGVMPDGTPFAIPEEYAAPLAIEIGTEVKETFVHLALP
                     LVRAGMPEFAVDAGPTTALSRFVGNDQRVEDNVQGVGDPAEMKLGQLNLRLMLDDDPR
                     SGFCSIAIARIVERRSTGQLILDESFIPPALECRGFSRLQDFLVEARNLVRHRADTLA
                     ARLNQSQQKGVGEIGDFMMLQVANRFDPWLSHLAERETLHPEALYAVLVQAAGEFATF
                     SERRKRRARVQVPYRHEDLKGTFEPLMFDFRELLTEVINPNAVPIPLQERAAGLYTGM
                     VPDPELLRTAQFVLAVNADKTSEFVRTRFPAQVKIGPPDKIRDLVRLHLPAIAVEPLP
                     MAPPRMPYYAGFTYFQLDRSTELWKQLDVTKVLALHIAGDFPGLQLELWALKP"
BASE COUNT          995 a         2386 c         2518 g         1028 t
ORIGIN      
        1 gcccgccctg cccggcccgc ccgcgaaggc gagcgtcagc gggaagtagc ggccgacgcg
       61 gtcgacgctc gcgaagaaca gcccgcgcca cgcgagcggc ccgcacacgc cgggcgcgag
      121 ctggaagcgc cagaccggcg ccgacaggta cagctccagc cagcgctcgc cgagtgcacc
      181 gcgcgaggcc tgcatgccct gttgcagcca gtcgtcccag gcggacgtga actcccacgg
      241 caggcgccgc gagacgaagt ccccctcgct ggggagcttg ccgtagaagc cgcaggtggc
      301 catggcgggt cgtcgacgaa aacggaaggc ctggacgcgg gacgccggag ggtgcggggc
      361 agatcagccc gggcagcgga actgcgcgag ctcgggcagg cgcagcgggt tcttcacgct
      421 gccggagcgc agctcgaacc agacgtgctt gtcgttgacc tggtagctga tcatctggcg
      481 ctcgggcgtg ccgccctcga ccttgccggc atcggcaaag cggaacagcg cccacggccc
      541 gtcgaagatc agcgggttgg tcgagggctt gccgccggcc accagcgtca gcttgatctg
      601 gctggtcggg ctcatgctgg gccagctcag gcgcatgccg gcgttgccgc ccggcgcgag
      661 gtgcgtgacc tggccgtcga ccgagacctg cacgtcggac acgccgtcgt ccgcgcgctg
      721 caggagcagg tcgaaggacg ccgacgggtt ggccgagccg cccgggaaga acgcatcgcg
      781 gatcaccgaa gcacgctgga agttcacgat cgtcgccggg tcgagcgagt tgacgccgtc
      841 ggcgatctgg atcggcttcc agaccgggcc attcatgtcg accatgccga ccagcttggt
      901 cttgacgaag ccgtcgatga cgccgttggg cgcgaacagg cgcgcgaagt cgcccagcgg
      961 cacttccttg gggacgccgc gcgtgaacgg atagcggccc tcgaccgctt gcttgcagaa
     1021 gctgcccacc tcgccggccg ccgcggcctt gacggccgcc tgcccggagc tggccgactg
     1081 cgccgtgctg cgcgagatga ggccgttgag caggttgctg atcggcggcg gcatgcgatc
     1141 cgcctcgctc ttgatgcgcg cgatgatcag cgtgtcggag gtcggcgtcg cgccgcgctt
     1201 gatcgcctcg tcggtcgcgc gcagctgcac ctcgtactcc ttgagcacgc ccagcgtggc
     1261 gtcgagcggc accggctggc ccgcggggcc ggcgacgacg cgctgcaact gctcgaagtg
     1321 ctggtcgacg atcgcctccg gcttggcctg ggcgtcgccc gtgtcggcgg gcttgctgcc
     1381 catcagcttg tcgaccgtgc tgcgcattgc gccggtgacc ttgtccatcg cgccggcggc
     1441 cgcggcctcg gcggagcgcg tgttgttgcc ggtgagcttg gtctccctcg cgacgccgtt
     1501 gagcagcagc ttcatcggcg agtcgctcgc cgagagcagc gtgatcgtct ggatgctctc
     1561 ctgcaggctg gtcgagggca cgaggcgcag gtcgccgagc aggtcgtccc agaccttgat
     1621 gtagtcgttc aggtacagct gctccacctg cgtgtaggtg gtcgcgatgc gcgcgtccgc
     1681 cgagcccgcg gcggggccca gcacccaggc ctgctcgtcg ccgagctgct tgaccatggg
     1741 cagcgcctgg cggcggaagc ccttgtcgaa cccggcgacc gtgtacagcc ccgggatgcc
     1801 ctgggtcagc ggcgtgccgg aggcgcgcgt gaacacgagc gccgcctgcg gcccgccggc
     1861 gtcgctgacg acgaagtcct tgacgccgtc gcccgcgccc aggcgcttca gacgcgaata
     1921 ggctcgctcg gccagcgacg aagccgccag ctgcttgcgc acctcgtcga tcagcgcctg
     1981 gtcgcgcggc agggccatct cgagcggcgt ctgcgccagc gccgcgtcga ggtggtcgcg
     2041 caggttgccg cgctgctgct gcgagatgtc gcgcggcagg gtgcggtccc agtcggcgcc
     2101 gatccaggcc tcgacgtcct gcgccttcag gtgcgtgggg tcgtacagct cgatgtacgt
     2161 cttcagcgcc tcgtagcgca cctcgggcga ctgggcgtcg cggatctgct gctccaggcg
     2221 cagcgcgatg cggggcatga acacgtcgcg cagcaggttc aggtaggcgg cgttcgcctg
     2281 cgtgccgagc ttgccggcct ggctcaggcc gaagctgcgc gaccacggcg tcggcgcgtc
     2341 acggtcggca tagccggtcg ggatcgcgcg cgccgcatcc agcagcggca gcacggtcat
     2401 cacgtcgccc accgtcaccg ggggcgtggc ctgcacctgc ttggagacgt cctgctgcgc
     2461 cgcgctgacc tcggagatca gcgcctcgtt gcgcacgaag ctgatcgtcc agccggcgat
     2521 gagcaaaacc gcgaacagcg cgagcagcga atacgcgacc accgcgacgc ggctgcggcg
     2581 ccgctcgatc accgcgctgc ggccgctgat gtcggattcg gcgaagacga cgctgcgcag
     2641 caggtgcgtg aggaagtagc tgcggcccga cgcggcggcg ggcgcgagca cctggcgctc
     2701 caggccgtac gcgcgcgaga tgttgcccag gacgcggtcg aacggactgc cctcctgggt
     2761 gccgctcgtg aagtagacgc cgcggaccat cggttcctcg ttgaacgagg agctcaggaa
     2821 cgcctcgtcg aggaaggccc tgagcatctc cctgagcgcg gcgaactgct gcgggaacgc
     2881 gaagagggtc gcgcggcgct gcgggtcgcg ctcctcttcc atgcgcgtgg tcaggcgcgc
     2941 gttgaggcgg ccgacgagcg cgtcgaactc gcgcccgaag gtctcgccca gcgcggcgcc
     3001 gacgggcatc gggaagtcga aggtcgtgcc ccagacctgc gcgcgggcct cgcggccgag
     3061 gtcgccgaag aactcgctga agcccgccag caggtcggtc ttcgtcacca tcacgtagat
     3121 cgggaagcgc acgccgagcg tcgagtacag ctcctggatg cgcgcgcgca ccgagcgcgc
     3181 gtactcgtcg cgctgcgccg cggactgcgt gaacaggtcg gacaggctca ccgtcacgat
     3241 cgcgccgttg agcggctgcg ccggacggaa cttcttcagc agcgtgagga agccggtcca
     3301 cgcggcggcg tcgacctcgc ggttgctgct ctgggtcgta taccgtccgg ccgtatcgag
     3361 cagcaccgcc tcgtcggtga accaccagtc gcagttgcgc gtgccgccga tgcccttgac
     3421 ggcgtccttg cccatcttgc cctcgagcgg gaagcgcagg ccggagttga cgagcgccgt
     3481 cgtcttgccg gagccggggg cgccgatgaa catgtaccag ggcagctggt agaggtagtt
     3541 gcgcccaccg cccgacttgt cgtcgaagcg cgccttgcgc agcgtgtcca tcgcctcgtc
     3601 gaagcgcttc ttcagctccg cgacctcgcg ctgcgacagc gccgagttgc tggacgcgcc
     3661 ctcgccgatc gcagacagca gctgcttgtt cgcgcgccag gcgcgccagc gcttgacgcc
     3721 ttcccagacg gcgaagcaaa ggatgatgaa caggatgagg ccgagccgcg cgcccgcgga
     3781 gccgagcggc cggtggtcat agaacgcgat cgcgtcgccg acgaaccaga tcagcagcga
     3841 gaccaccagc agcccgatga ccgccagcac ccaacgattg gaaaagaacg ccttgagctt
     3901 cgcccacatg tcttgttgtc ccgcttaggt cccggtacga agggtgatct cgacgcggcg
     3961 gttctgcgcg cggcccgcgg gcgtcgtgtt gggcacgagc ggcttgctgt cggccagccc
     4021 ctccgccttg atcgtgtgct tctgcaccgt ctgcgcgagc agcgcggcga cgccgcgggc
     4081 acgctccagc gagagctcgt aattcgacgg gaagcgcagc gagcgcttgg tcggctggtt
     4141 gtccgtgtgc ccgctgaccg tcaccacgcc cggcacctgg ttgatcgcct gcgcgacgcg
     4201 cgtgatcacc ggcacgagct ccggccggat ctcggcgctg cccgggtcga agaacttgtc
     4261 gccgtggatc acgacgtggc tcgaggtggc gtcctcgtcg acgtccagca gcccctgcgc
     4321 gatctcggcg gccagcagcg gcttcaggcg cggcgcggcc ggcggcggcg cgggcggcgg
     4381 cggccgcttg aggtcgccga cgtccgagtg gatcgagccc agcgcggaga acaccgggtc
     4441 ggacttcgag ccgagcacga gcgcgaacac gagatacagc acgaacagca gcagcgccac
     4501 cgcggcgagc acgatccacg ccgacagccg gcgcacgagc ggcttggcct ggcgctgctg
     4561 gcctttccac ttgccggaga ggtcgcgctc gaactcgccg cgctcgcggc ggatcacgtc
     4621 gtgcaggcgg tcgcgcaccg cgtcgagctg cgcgcggccg ttctccatca cgctgtagcg
     4681 cccttcgaag ccgagcgcga ggcagacgta catcagctcc agcagctcga ggttgcggcg
     4741 cgggtcctcc atcgccttgt ccaggagctg gaagaacttc tcgccgccga agccctcgcg
     4801 gtgcagcgtc acgagcaggc tctgctgcac ccactcgggg ctcgcgcccc agggcatgtt
     4861 ggcgacggcc tcgtcgatca tcgtgcacag cgcgtagcgc gcgatcagca cgtgctcggg
     4921 gctgctgccg gcggcgcggg cgcgcgcctc gaaatcggtg atcgcgcgca gcaggtaggc
     4981 gcgcaggccg gcgggatccg cgtggttcag cgtgcggcga atggtcggca ccgcgttcag
     5041 cagcacggag gcgttggcca ccagcgggtt gaggccgacg ggcgcggcgg attcctcgac
     5101 gtcggagagc acctgcgcgg tgtccagcga cacggcctgc cccgcggtgg gtgccggccc
     5161 ggccgggcgg cggccgcccg gcttcgggat catgatcgtg gattccgagt cgggctgcgg
     5221 cttgttggga ggcagccccg gcaggttggg aaacggcggt tggctcatga ggggactcgc
     5281 ttcgcggcgc ccggcgccac gaggtttggg ctcgggatct gcgacctcag ggcttcaggg
     5341 cccagagctc cagttgcagg ccggggaagt cgcccgcgat gtgcagggcg agcaccttgg
     5401 tcacgtcgag ctgcttccac agctcggtgc tgcgatcgag ctggaaatag gtgaagccgg
     5461 cgtagtacgg catgcgcggc ggcgccatcg gcagcggctc caccgcgatc gcgggcaggt
     5521 gcaggcgcac gaggtcgcgg atcttgtcgg gcgggccgat cttgacctgc gcggggaagc
     5581 gcgtgcgcac gaactcgctg gtcttgtcgg cgttgaccgc cagcacgaac tgcgcggtgc
     5641 gcagcagctc cgggtcgggc accatgccgg tgtagaggcc ggcggcgcgc tcctgcagcg
     5701 ggatgggcac cgcattcgga ttgatgacct cggtcagcag ctcgcggaag tcgaacatca
     5761 gcggctcgaa ggtgcccttg aggtcctcgt gccggtaggg cacctgcacg cgcgcgcggc
     5821 gcttgcggcg ctcgctgaag gtggcgaact cgcccgcggc ctgcaccagc accgcgtaca
     5881 gcgcctcggg atgcagcgtc tcgcgctcgg cgagatggct cagccacgga tcgaagcggt
     5941 tggcgacctg cagcatcatg aagtcgccga tctcgccgac gcccttctgt tgcgactggt
     6001 taaggcgcgc cgccagcgtg tcggcgcgat ggcgcacgag gttgcgcgcc tcgaccagga
     6061 agtcctgcag gcgcgagaag ccgcggcatt ccagcgcggg cgggatgaag ctctcgtcga
     6121 ggatcagctg gcccgtgctg cggcgctcca cgatgcgcgc gatggcgatg ctgcagaagc
     6181 ccgagcgcgg gtcgtcgtcc agcatcaggc gcaggttcag ctggccgagc ttcatctccg
     6241 ccgggtcgcc gacgccctgc acgttgtcct cgacgcgctg gtcgttgccg acgaagcgcg
     6301 agagcgcggt cgtcgggccg gcgtcgaccg cgaactccgg catgcccgcg cgcacgagcg
     6361 gcagcgcgag gtgcacgaag gtctccttga cctcggtgcc gatctcgatg gccaggggcg
     6421 cggcgtattc ctccgggatg gcgaacggcg tgccgtccgg catcacgccg cgcgcggcct
     6481 tcaccgcgag gcggccgatc ttcaaggcct cgtcgtcgag gcgcagcgtg ccgagacccc
     6541 aggccggcgc gaacacgccg tgggtgcgcg cgtcgaccag catctccacc gaccgttcga
     6601 gctgctggaa atgctgcggc cgcaggaaca gtccctcgga ccagatgacg cgcttacgac
     6661 tcatgctctt cttgcctcgc caggcgcgcg gtcgcgcgcg ttttcgggtc tcgccatccc
     6721 tctcggaggc ggcgccaact gtacacgcaa accacttcgc cgccgagggt ggaaagcacc
     6781 cctttccggg ggatgcggag gtgcacgcgc agggccgcga gggccttcgc cgatgcgtgg
     6841 cgagcgcgca cccgccatcc cgggcgagcg cgcgcgcggg tcgctcaggc ctcgcgccag
     6901 cggccctgca gcgggccgtt gcccggc
//
LOCUS       DASVLN010000207         4421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_385917, whole genome shotgun sequence.
ACCESSION   DASVLN010000207 DASVLN010000000
VERSION     DASVLN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385917"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /gene="urtB"
                     /locus_tag="VF453_04695"
     CDS             <1..985
                     /gene="urtB"
                     /locus_tag="VF453_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX7686982.1"
                     /translation="APDGEADPQLRAALAAALAQVDGRLAWGERLGLLFTGLSLGSIL
                     LLVALGLAITYGLMGVINMAHGELMMIGAYATWLVQGLFRRWAPQWFDAYLVLAVPAA
                     FLAAALVGAVLERGVIRFLYGRPLETLLATWGISLVLMQLVRTLFGAQNVEVENPSWM
                     SGGVAVMSNLVLPWNRLAILAFALAVLGATALVIGRTRLGLFVRGVTQNRRMASCTGI
                     DTARTDTMAFALGAGIAGLAGCALSQVGNVGPDLGQGYIVDAFMVVVLGGVGQLAGTV
                     LAALGLGLMGKALEGVAGAVLAKILVLVFIVVFIQKRPQGLFALKGRSAES"
     gene            1047..2195
                     /gene="urtC"
                     /locus_tag="VF453_04700"
     CDS             1047..2195
                     /gene="urtC"
                     /locus_tag="VF453_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEX7686983.1"
                     /translation="MSPAASPVPAVHLLPEARRGLLLGVRGWIAVAAALALVAIVVPV
                     LNLLVPAGSPLHLGDWGVALVAKIMCYAICALAMDLVWGYAGILSLGHGLYFALGGYA
                     MGMYLMRQIGRDGNYRSDLPDFMVFLDWKTLPWTWAGSASLGFQLFLVVAAPSVVAGV
                     FGWFAFRSRIKGVYFSIITQALTFAAMLLFFRNETGFGGNNGFTDFKRIAGVPIALPS
                     TRMALFVITGCTLVGFVLMARAIVNSKFGRVLQAIRDAESRVMFTGYDPLRYKLAVWV
                     LSAAMCGVAGALYVPQVGIVNPSEMSPASSIEMAVWCAVGGRGTLVGPVLGAFFVNGA
                     KSWFTQAFPEFWLYFLGALFIAVTLFLPDGLLGIAASLRREKGVEARR"
     gene            2192..3055
                     /gene="urtD"
                     /locus_tag="VF453_04705"
     CDS             2192..3055
                     /gene="urtD"
                     /locus_tag="VF453_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEX7686984.1"
                     /translation="MTPELMDEGAAVLERARARATPAPSGGASFGRVVQRHVPDFAHG
                     VALYLDDISVSFDGFKALDALSLAIDVGELRCIIGPNGAGKTTMMDVITGKTRPDAGR
                     AHFGTNIDLLRLREPEIVRAGICRKFQKPTVYEELSVFENLELALDADRGVRATLTAR
                     MSAAQRERIAGILELVHLLPEARRLAGLLSHGQKQWLEIGMLLAQEPKLLLVDEPVAG
                     MTDSETVQTAELLKEINRTKTVVVVEHDMAFVRELGVKVTCLHEGSVLAEGSIDQVSQ
                     NERVVEVYLGR"
     gene            3059..3754
                     /gene="urtE"
                     /locus_tag="VF453_04710"
     CDS             3059..3754
                     /gene="urtE"
                     /locus_tag="VF453_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEX7686985.1"
                     /translation="MLEVTNIDLHYGAAQALRGISLKAEVGKVTCVLGRNGVGKTSLL
                     RAIVGQRPVSAGAITLEGRDITKLTTVERARHGVAIVPQGREIFPLLTVEENLETGFA
                     PLKRDQRSVPDDVFSLFPVLNSMLGRRGGDLSGGQQQQLAIGRALVMRPRLLLLDEPT
                     EGIQPSIIKDIGRAISYLRGLKEMAIVLVEQYLDFAQELGDHLVVMDRGSIVYSCDKA
                     SMDEGALKRALAI"
     gene            3805..>4421
                     /locus_tag="VF453_04715"
     CDS             3805..>4421
                     /locus_tag="VF453_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX7686986.1"
                     /translation="MNAAERQAIFAANRATGHIALGVAAHAGRTHRTRVHEDGSLRVR
                     FPNAPGDMLEAVIVNTGGGMTGGDRFAIDIVLEEGASLIAGTAAAEKIYRSTGPDTEM
                     DVTLTVGANARLVWLPQETILFDRARLKRRIDIDLAGSASLLMAEAVVFGRAAMGEAI
                     EQGFFADRWRVRRAGRLVFADSARLDGAIAGKLAAVASAAGGIAIA"
BASE COUNT          610 a         1494 c         1573 g          744 t
ORIGIN      
        1 cgcgcccgac ggcgaggcgg atccgcagct gcgcgccgcg ctcgccgccg cgctcgccca
       61 ggtcgacggg cgcctcgcct ggggcgagcg cctgggcctg ctgttcaccg gcctgagcct
      121 gggctcgatc ctgctgctcg tcgcgctggg cctggcgatc acctacggcc tgatgggcgt
      181 gatcaacatg gcgcacggcg agctgatgat gatcggcgcc tacgccacct ggctcgttca
      241 gggcctcttc cgccgctggg cgccgcagtg gttcgacgcc tacctggtgc tcgccgtccc
      301 cgcggccttc ctcgccgccg ccctcgtcgg cgcggtgctg gagcgcggcg tgatccgctt
      361 cctctacggc cggccgctcg agacgctgct ggcgacctgg ggcatcagcc tcgtgctgat
      421 gcagctggtg cgcacgctgt tcggcgcgca gaacgtcgag gtggagaacc cgtcgtggat
      481 gtcgggcggc gtcgcggtca tgagcaacct cgtgctgccc tggaaccggc tcgcgatcct
      541 cgccttcgcg ctggcggtgc tcggcgccac cgcgctggtg atcggccgca cgcggctggg
      601 cctgttcgtg cgcggcgtga cgcagaaccg ccgcatggcc tcgtgcacgg gcatcgacac
      661 cgcgcgcacg gacacgatgg ccttcgcgct gggcgcgggc atcgcgggcc tggccggctg
      721 cgcgctgagc caggtgggca acgtcggccc cgacctcggc cagggctaca tcgtcgacgc
      781 gttcatggtg gtggtgctgg gcggcgtcgg ccagctcgcg ggcacggtgc tcgcggcgct
      841 cggcctgggg ctgatgggca aggcgctcga gggcgtcgcc ggcgcggtac tggccaagat
      901 cctcgtgctg gtcttcatcg tcgtgttcat ccagaagcgg ccgcagggcc tgttcgccct
      961 caagggccgg agcgccgaat cgtgagtgcc cgtcccgccg cccgcgaggg cgatcgcatc
     1021 gcaggccgcc aggccggagg tatccgatga gtcccgccgc aagtcccgtg cccgcggtcc
     1081 acctgctgcc cgaagcccgc cgcggcctgc tgctgggcgt gcgcggctgg atcgccgtgg
     1141 cggcggcgct cgcgctggtg gcgatcgtcg tgccggtgct gaacctgctc gtgccggccg
     1201 gcagcccgct gcacctgggc gactggggcg tcgcgctggt ggccaagatc atgtgctacg
     1261 cgatctgcgc gctcgcgatg gacctggtct ggggctacgc cggcatcctc tcgctgggcc
     1321 acggcctgta tttcgcgctc ggcgggtacg caatgggcat gtacctgatg cgccagatcg
     1381 gccgcgacgg caactaccgc agcgacctgc ccgacttcat ggtgttcctg gactggaaga
     1441 cactgccctg gacctgggcc ggcagcgcgt cgctcggctt ccagctgttc ctggtcgtcg
     1501 cggcgccgtc ggtggtggcc ggcgtgttcg gctggttcgc gttccgctcg cgcatcaagg
     1561 gcgtgtactt ctcgatcatc acgcaggcgc tgaccttcgc cgcgatgctg ctcttcttcc
     1621 gcaacgagac cggcttcggc ggcaacaatg gcttcaccga cttcaagcgc atcgcgggcg
     1681 tgccgatcgc gctgccctcg acgcgcatgg ccctcttcgt catcacgggg tgcacgctcg
     1741 tgggcttcgt gctgatggcg cgcgcgatcg tcaactcgaa gttcggccgc gtgctgcagg
     1801 cgatccgcga cgcggagagc cgcgtgatgt tcaccggcta cgacccgctg cgctacaagc
     1861 tcgcggtctg ggtgctgtcg gcggcgatgt gcggggtcgc cggcgcgctc tacgtgccgc
     1921 aggtgggcat cgtcaacccg agcgagatgt cgccggcgtc ctcgatcgag atggcggtct
     1981 ggtgcgcggt gggcgggcgc ggcacgctgg tcgggcccgt cctcggcgcg ttcttcgtca
     2041 acggcgcgaa gagctggttc acccaggcct tccccgaatt ctggctgtac ttcctcggcg
     2101 cgctgttcat cgcggtgacg ctgttcctgc ccgacgggct gctcggcatc gcggcgagcc
     2161 tgcggcgcga gaagggcgtg gaggcccggc gatgaccccc gagctgatgg acgaaggcgc
     2221 cgccgtcctc gagcgcgccc gcgcgcgcgc gacgccggcg ccttcgggcg gcgcgagctt
     2281 cggccgcgtc gtgcagcgcc acgtgccgga cttcgcgcac ggcgtcgcgc tctacctgga
     2341 cgacatcagc gtgagcttcg acggcttcaa ggcgctcgac gcgttgtccc ttgccatcga
     2401 cgtcggcgag ctgcgctgca tcatcggccc caacggcgcg ggcaagacga cgatgatgga
     2461 cgtgatcacc ggcaagaccc gccccgatgc cgggcgcgcg cacttcggca cgaacatcga
     2521 cctgctgcgc ctgcgcgagc ccgagatcgt gcgcgccggc atctgccgca agttccagaa
     2581 gcccaccgtc tacgaggagc tgtcggtgtt cgagaacctc gagctcgcgc tcgacgccga
     2641 ccgcggcgtg cgcgcgacgc tcacggcacg gatgagcgcc gcgcagcgcg agcgcatcgc
     2701 cggcatcctg gagctcgtgc acctgctgcc cgaggcgcgc cggctggccg gcctgctgtc
     2761 gcacgggcag aagcagtggc tggagatcgg catgttgctg gcgcaggagc cgaaattgct
     2821 gctggtcgac gagccggtcg ccggcatgac cgacagcgaa accgtgcaga ctgccgaatt
     2881 gcttaaggag atcaacagga ccaagacggt ggtggtggtc gagcacgaca tggcctttgt
     2941 gcgcgaactc ggcgtcaagg tcacctgtct gcacgagggc tcggtgctgg cggaaggctc
     3001 gatcgaccag gtatcgcaga acgagcgcgt tgttgaagtt tacttaggac gctgaccgat
     3061 gctcgaggtg accaacatcg atctgcacta tggcgcggcg caggcgctgc gcggcatttc
     3121 gctcaaggcc gaagtgggga aggtgacctg cgtgcttggc cgcaacggcg tcggcaagac
     3181 cagcctgttg cgtgccattg tcggccagcg gccggtgagc gccggcgcga tcaccctcga
     3241 gggcagggat atcaccaagc ttacgaccgt cgagcgcgcg cgccacggcg tcgccatcgt
     3301 gccgcagggg cgtgagattt tcccgctgct gaccgtcgag gaaaacctgg agaccggttt
     3361 tgcgccgctc aagcgcgacc aacgctcggt gccggacgac gtgttctcgc tgttcccggt
     3421 gctcaacagc atgctcggcc ggcgcggcgg cgatctctcc ggcggccagc agcaacaatt
     3481 ggcgatcggc cgcgccttgg tgatgcggcc gcgcctcttg ttgctcgacg agccgaccga
     3541 aggcatccag ccttcgatca tcaaggacat cggccgcgcc atctcctacc tccgcggtct
     3601 caaggaaatg gctattgtgc tggtcgagca gtacctcgac ttcgcgcagg aactcggcga
     3661 ccatctggtg gtcatggacc gcggatcgat cgtctattcg tgtgacaagg cgagcatgga
     3721 cgaaggggcg ctcaagcgcg cgctggcgat ttgagggcga tgagcgctat gctttcggcc
     3781 gtaggcgtca aagaggcacc gttcgtgaac gcggcagaac gacaagcgat cttcgccgcc
     3841 aatcgcgcga ccgggcacat cgcgctcggc gtggcggcac acgccggccg cacgcaccgt
     3901 acccgcgtgc acgaggacgg ctcgctgcgc gtgcgatttc ccaacgcgcc aggcgacatg
     3961 ctggaagcgg tcatcgtcaa caccggcggc ggcatgacgg gcggcgaccg ctttgccatc
     4021 gatatcgtgc tggaagaagg ggcgagcctg atcgccggca ccgcggcggc ggaaaagatt
     4081 tatcgctcca ccggaccgga caccgaaatg gatgtgacgc tgacggtcgg cgccaacgcc
     4141 aggcttgtct ggctgccgca ggaaaccatt ctgttcgacc gcgcgcgtct taagcgccgc
     4201 atcgatatcg atctggccgg aagcgcctcg ctgctgatgg cggaggcggt ggtgttcggc
     4261 cgtgcggcca tgggcgaggc aatcgagcag ggttttttcg ccgaccgttg gcgcgtgcgc
     4321 cgcgctggcc ggctcgtgtt cgccgacagc gcgcggctcg acggcgccat cgccgggaag
     4381 ctggcggcgg tcgcttcggc cgccggcggt attgctatcg c
//
LOCUS       DASVLN010000208         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_332933, whole genome shotgun sequence.
ACCESSION   DASVLN010000208 DASVLN010000000
VERSION     DASVLN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332933"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1809
                     /locus_tag="VF453_04720"
     CDS             <1..>1809
                     /locus_tag="VF453_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7686987.1"
                     /translation="AVDSGSAAAASSAIGAVVGAETATFKPADELQRLQLVGRSQPDV
                     ALPGLLHYADTLRPDDPRHLETLMELGDVYVSLADGERVEQVAHRIEQLGVPLARPAA
                     MVLRGHWLQAHDEVSRAERQFIEAAALLPPDAPASLRLRLLTSWAEVKSRGGHYDAAL
                     ARYNQALKLIDDTGPPWRRIDMRGSIASVLMDANQFDKAAEFVEAMMRLATEAGDELG
                     MSRAYTLRGFTLSRGPDSAAALAADRTALEHARRSGNQRQVVLCMANVADYYLSHGDF
                     QTAWDISQRALPIARRLNSESVESLALANTGLALIGMHRKEEGLPLVHESMSMDERSG
                     SARSISDEAYELGGYLERAGYLGDALDAYRRYRRMSDELDQQDRQRALIELQEGFANE
                     SRQHELEMLSREGRLKDAEILHHELRIKLWTAAGLSSLLLAAVIATLARRLRVRNQML
                     SVSNEQLRQQAEIDPLTGLSNRHHLQAVMARRPGPGLQGTLYLLDVDHFKQINDRCGH
                     AGGDAVLIEIARRLRATLRDDDLVVRWGGEEFLVLVRPLPPAEAEALALRLLGALADA
                     PVPHDGQPVAVSASIGYAGFPLASSAPQPELVVPWE"
BASE COUNT          256 a          679 c          644 g          230 t
ORIGIN      
        1 cggccgtcga cagcggatcc gcggccgcgg cgtcgtcggc catcggggcg gtcgtcggcg
       61 ccgagaccgc gaccttcaag ccggccgacg agctgcagcg cctgcaactc gtcggccgca
      121 gccagccgga cgtcgccctg cccggactcc tgcactacgc cgacaccctg cgtcccgacg
      181 atccgcgcca cctcgagacg ctgatggagc tgggcgacgt ctacgtcagc ctggccgacg
      241 gcgagcgcgt cgagcaggtc gcccatcgca tcgagcagct gggcgtgccg ctcgcccgtc
      301 cggcggcgat ggtgctgcgc gggcactggc tgcaggccca cgacgaggtg agccgcgccg
      361 agcgccagtt catcgaggcc gccgcgctgc tgccgcccga cgctccggcc agcctgcggc
      421 tgcggctgct cacgagctgg gccgaggtca agagccgcgg cggccactac gacgcggcgc
      481 tcgcgcgcta caaccaggcg ctcaagctga tcgacgacac cgggccgccg tggcgccgca
      541 tcgacatgcg cggctcgatc gcgagcgtcc tgatggacgc caaccagttc gacaaggccg
      601 ccgagttcgt cgaggcgatg atgcggctgg ccaccgaggc cggcgacgag ctggggatgt
      661 cgcgcgccta cacgctgcgc ggcttcaccc tgagccgcgg cccggacagc gcggccgccc
      721 tggccgccga ccgcacggcg ctcgagcatg cgcggcgctc gggcaaccag cgccaggtcg
      781 tgctgtgcat ggccaacgtc gccgactact acctctcgca cggcgacttc cagactgcgt
      841 gggacatctc ccagcgcgcg ctgcccatcg cccgccggtt gaacagcgag tccgtcgaga
      901 gcctggcgct cgccaacacg ggcctcgcgc tgatcggcat gcaccgcaag gaagagggcc
      961 tgccgctggt gcacgagtcg atgagcatgg acgagcgctc cggatccgcg cgcagcatct
     1021 ccgacgaggc ctacgagctg ggcggctacc tcgagcgcgc cggctacctc ggcgacgccc
     1081 tcgacgccta ccgccggtac cgtcgcatgt ccgacgagct cgaccagcag gatcgccagc
     1141 gcgcgctgat cgagctgcag gagggcttcg ccaacgagag ccgccagcac gaactggaga
     1201 tgctgtcgcg cgagggccgg ctcaaggacg ccgagatcct gcaccacgag ctgcgcatca
     1261 agctgtggac ggccgcgggg ctgtcgagcc tgctgctcgc cgccgtgatc gcgacgctcg
     1321 cgcgccggct gcgggtgcgc aaccagatgc tgtcggtcag caacgagcag ctgcgacagc
     1381 aggcggagat cgacccgctg acgggcctgt ccaaccgcca ccacctgcag gccgtgatgg
     1441 cccggcgtcc cggtcccggc ctgcagggca cgctgtacct gctggacgtc gaccacttca
     1501 agcagatcaa cgaccgctgc ggccacgccg ggggcgacgc ggtgctgatc gagatcgcgc
     1561 gccgcctgcg ggcgacgctg cgcgacgacg acctcgtcgt gcgctggggc ggcgaggagt
     1621 tcctggtgct cgtgcgcccg ctgccgccgg ccgaggccga ggcgctcgcg ctgcggctgc
     1681 tgggcgcgct cgccgacgcg ccggtgccgc atgacggcca gccggtggcg gtgtcggcct
     1741 cgatcggcta cgccggcttc ccgctcgcct cgtcggcgcc gcagccggaa ctcgtcgtgc
     1801 cctgggagc
//
LOCUS       DASVLN010000209         2806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_171975, whole genome shotgun sequence.
ACCESSION   DASVLN010000209 DASVLN010000000
VERSION     DASVLN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171975"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VF453_04725"
     CDS             complement(<1..259)
                     /locus_tag="VF453_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686988.1"
                     /translation="MSVPSSSLPGASDAAAAAPQPRTIHAAPASVAGTAVRQDLVHPD
                     PLLDCLVEVCRLHGQHATRASLSAGLPLVDGRLTLALAERAA"
     gene            complement(314..859)
                     /locus_tag="VF453_04730"
     CDS             complement(314..859)
                     /locus_tag="VF453_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7686989.1"
                     /translation="MFSPRSIRHCRACGAPTEYRMPDEDNRERAVCTACSTIHYENPL
                     NVVGTVPELDGRVLLCKRNIEPRRGLWTLPAGFMELGETTAQGALRETVEEAGAEVEI
                     GPLFTVLDVPHVSQLHLYYLARMTSTRLDPGPETMEARLFTEDEVPWEQLAFRTVRFT
                     LERFFADRRRGQFGMHRADIA"
     gene            999..1703
                     /locus_tag="VF453_04735"
     CDS             999..1703
                     /locus_tag="VF453_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7686990.1"
                     /translation="MTDVITVPAIPRVPVHGGGTFPVHRIYCVGRNYAEHAAEMGHDA
                     REQPFFFSKPADAVLPVADGETGRMDYPTLTADLHHELELVVAIGDAAAGNGRDVAEE
                     DAPRLIFGYAIGLDMTRRDLQATAKKMGRPWSIAKGFDQSAPIGPIHRIEETGELTAG
                     EIALEVDGAPRQRGDLSDMIWNVRETIAWISRAWMLRPGDLIFTGTPAGVAAVGRGQV
                     MRGRIAKLGELVVTLR"
     gene            1736..2371
                     /gene="maiA"
                     /locus_tag="VF453_04740"
     CDS             1736..2371
                     /gene="maiA"
                     /locus_tag="VF453_04740"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX7686991.1"
                     /translation="MELHNYFRSSASWRVRIALALKGLPYDYRPVHLVRQEQLAPAFV
                     EASSTGLVPLLVTDGGERLHQSLALIEYLDEIHPEPPLLPADAAGRARVRALALDIAC
                     EVHPLNNLRVLQYLVRTVGASEEQKIAWIRHWIESGLAAVERQLAARPSTFCHGESPG
                     LADCTLVPQIFNAQRFGARLEHVPHVMRVHAACMAHPAFASTQPSACPDAE"
     gene            2382..>2806
                     /locus_tag="VF453_04745"
     CDS             2382..>2806
                     /locus_tag="VF453_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HEX7686992.1"
                     /translation="MPVEIVEPLARADLIWPPDWPRGTGAVMSTRAGGVSAAPFDSLN
                     LRAQSEDPAELIAVAENRRRFAASLGAQPVFLDQVHGTRVVRLDASHLAAGAVPERAD
                     ASVTTVPGIACTVLVADCLPVLFATRDGRAVGAAHAGWRG"
BASE COUNT          367 a         1043 c         1027 g          369 t
ORIGIN      
        1 cggcggcgcg ctcggccagg gccagggtga gccggccgtc gaccagcggc aggccggctg
       61 acagcgaggc gcgcgtcgcg tgctggccgt gcaggcggca gacctcgacc aggcagtcga
      121 gcagcgggtc gggatgcacg aggtcctggc gcacggcggt gccggccacc gaggcgggcg
      181 cggcgtggat cgtgcgtggc tgcggcgccg cggcggcggc gtcggaggct ccgggcagcg
      241 aggaagaggg gacgctcatc gcgggaagag ggccggtcgg ccgggatcgt cgggcgccgc
      301 catcggcgcc tcgtcacgcg atatcggcac gatgcatccc gaattgacct cggcggcggt
      361 cggcgaagaa gcgttccagg gtgaaacgca cggtccggaa ggcgagctgc tcccagggca
      421 cctcgtcctc ggtgaacagg cgcgcctcca tggtctcggg gccgggatcc aggcgggtgc
      481 tggtcatgcg cgccaggtag tacaggtgca gctggctgac gtgcggcacg tcgagcacgg
      541 tgaacagcgg gccgatctcg acctcggccc ccgcttcctc gaccgtctcg cgcagcgcgc
      601 cctgggccgt ggtctcgccg agctccatga agccggcggg cagcgtccag aggccgcgcc
      661 gcggctcgat gttgcgcttg cacagcagga cgcggccgtc gagctcgggg acggtgccga
      721 cgacgttgag cgggttctcg tagtggatcg tcgagcaggc cgtgcagacc gcgcgctcgc
      781 ggttgtcctc gtcgggcatg cggtactcgg tcggagcgcc gcaggcacgg cagtggcgga
      841 tggaacgtgg ggagaacatg gtcgcggccc tcggtgaagc gcagtgtagc cggcggcccc
      901 gcggcgaccc cggccgcgag ggggttcatc gccccgcatg gcatcattgc ccgccgtcgg
      961 gggccgccgc ggtccccgca acgatccgag gcccgaccat gaccgacgtc atcaccgtcc
     1021 ccgccatccc gcgcgtgccc gtgcacggcg gcggcacctt ccccgtccac cgcatctact
     1081 gcgtcggccg caactacgcg gagcacgcgg ccgagatggg ccacgacgcg cgcgagcagc
     1141 cgttcttctt ctccaagccg gccgacgccg tgctgccggt cgccgacggc gagaccgggc
     1201 ggatggacta cccgacgctc accgccgacc tccaccacga gctcgagctg gtcgtggcga
     1261 tcggcgacgc cgccgccggc aacggccgcg acgtcgcgga ggaggacgcc ccgcgcctga
     1321 tcttcggcta cgcgatcggc ctggacatga cgcgccgcga cctccaggcc acggccaaga
     1381 agatgggccg gccgtggtcg atcgccaagg gcttcgacca gtcggcgccg atcgggccga
     1441 tccaccgcat cgaggagacc ggcgagctga ccgccgggga gatcgcgctg gaggtcgacg
     1501 gcgcgccgcg ccagcgcggc gacctgtccg acatgatctg gaacgtgcgc gagaccatcg
     1561 cctggatcag ccgcgcctgg atgctgcggc cgggcgacct gatcttcacc ggcacgccgg
     1621 cgggcgtcgc ggcggtgggc cgcggccagg tgatgcgcgg gcgcatcgcg aagctgggcg
     1681 agctcgtcgt cacgctgcgc tgatccgccg cgtacccgta cgtcgcgtcg ccgccatgga
     1741 actgcacaac tacttccgct cctcggccag ctggcgcgtg cgcatcgcgc tcgcgctcaa
     1801 gggcctgccc tacgactacc ggcccgtcca cctggtgcgc caggagcagc tggcgccggc
     1861 cttcgtcgag gcctcgtcca ccggcctcgt gccgctgctc gtgaccgacg gcggcgagcg
     1921 cctgcaccag tcgctcgcgc tcatcgagta cctcgacgag atccatcccg agccgccgct
     1981 gctgccggcc gacgccgccg gccgcgcacg cgtgcgtgcg ctcgcgctgg acatcgcgtg
     2041 cgaggtccac ccgctcaaca acctgcgcgt gctgcaatac ctggtcagga cggtgggcgc
     2101 gagcgaggag cagaagatcg cctggatccg ccactggatc gagtccggtc tcgccgcggt
     2161 ggagcgccag ctcgccgcgc ggccgtccac cttctgccac ggcgaatccc ccgggctcgc
     2221 cgattgcacc ctggtgccgc agatcttcaa cgcgcagcgc ttcggggcgc ggctggagca
     2281 cgtcccgcac gtcatgcgcg tgcacgcggc gtgcatggcg cacccggcgt tcgcgtccac
     2341 gcagccgtcg gcctgtcccg acgccgaatg aagggatccc cgtgcccgtc gagatcgtcg
     2401 aacccctggc gcgtgccgac ctgatctggc cgcccgactg gccgcgcggc accggcgcgg
     2461 tcatgagcac gcgcgccggc ggcgtcagcg ccgcgccctt cgactcgctg aacctgcgcg
     2521 cgcagtccga ggatccggcc gagctcatcg ccgtcgccga gaaccgccgg cgcttcgccg
     2581 cctcgctggg cgcgcagccg gtcttcctcg accaggtgca cggcacgcgg gtcgtccggc
     2641 tcgacgcttc ccatctggcc gccggcgccg tgcccgagcg cgccgacgcc agcgtcacga
     2701 ccgtgccggg catcgcgtgc accgtgctcg tcgccgactg cctgccggtg ctgttcgcca
     2761 cgcgcgacgg ccgcgcggtc ggcgccgcgc acgcgggctg gcgcgg
//
LOCUS       DASVLN010000210         2626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_279619, whole genome shotgun sequence.
ACCESSION   DASVLN010000210 DASVLN010000000
VERSION     DASVLN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279619"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1107)
                     /locus_tag="VF453_04750"
     CDS             complement(181..1107)
                     /locus_tag="VF453_04750"
                     /EC_number="6.2.1.n2"
                     /inference="COORDINATES: protein motif:HMM:NF005479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--[acyl-carrier-protein] ligase"
                     /protein_id="HEX7686993.1"
                     /translation="MSLHETPDPYDPAAFRAGLVEHGFLLPTGVRGGDGRTAKFEDIL
                     ERFDALVMRETAGDGAQPLHFPPIVARALIERLGYLGNFPQLVGSIHSFFGDEKTARE
                     LAALADAGQRWEHLLEPAETMLLPAACYPVYPLFSGLVPAQGRLVTTRNWCYRHEPSD
                     EPTRLQSFRMREFVRVGAPEAVQAWRDAWLERGLALLRGLGLPAASDVASDPFFGRAG
                     RMMATSQRDQRLKFEIVVPVISAGKPTAVCSFNWHQEHFTSTFGIRRPDGALAHTACL
                     GFGLERVTLALIKAHGFDLDAWPAAVRAPLWG"
     gene            complement(1104..2327)
                     /locus_tag="VF453_04755"
     CDS             complement(1104..2327)
                     /locus_tag="VF453_04755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7686994.1"
                     /translation="MPNPDFARLLQEVRRISKEIAAPAAADVDAQARFPQEAVDALRA
                     ARVLSAAVPERLGGAGLGLAQLGQLCHALAQGCASSAMVLAMHTIQVACVARHAAGSA
                     EFEAVLRELAAEQRLFASMTSEAGTFGDTRTSICAIEPADDPARFLLAKDATTGSYCA
                     HADAILVTARRHAEAAPGDQVLAFLRATDATLRQVGSWDTLGMRGTCSPGFRLESSAP
                     RAQVLPVDYADISALTMVPWSHVLWAALWTGIATDAHARAAAFVRGQARRNPGSTPPT
                     ALRLAELTTTLQAMRHHWQSVAAEVDALPLPSTAVAPRAGAAVAAADEPHPLSRIAWS
                     LKFNSLKTAAAERAPEVVHGALQVVGILGYKNDTPFSLGRHYRDALSAALMISNDRIA
                     AQSANLLLVFKDDQE"
     gene            2389..>2626
                     /locus_tag="VF453_04760"
     CDS             2389..>2626
                     /locus_tag="VF453_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7686995.1"
                     /translation="MNDKDDDSVPDATTRAGVDTPQGVQPDVTWLEPAGPARAAVLLL
                     HGLDMTPAQLAPLLRSLRLPAWVALPAGPVERPGG"
BASE COUNT          339 a          916 c         1042 g          329 t
ORIGIN      
        1 atggcgcacg ccggccccgt ccagcgtcat gcggccggcg gccgcggcgc cggggcgcac
       61 ggccatggcg gtcggcgtgt ctccgtgggg ccgcggggcg acgggatcgg cctcggacgg
      121 ggacggcgcg ctgcgcctgt cgcggctgct catgatggaa gcttcccctt gcaagtcatc
      181 tcagccccac agcggtgcgc gcaccgcggc gggccaggcg tcgaggtcga agccgtgcgc
      241 cttgatcagc gcgagcgtca cgcgctccag gccgaagccc aggcaggcgg tgtgggcgag
      301 cgcgccgtcg gggcgacgga tgccgaaggt cgacgtgaag tgctcctggt gccagttgaa
      361 cgagcagacc gcggtcggct tcccggcgga gatcacgggg acgacgatct cgaacttcag
      421 ccgctggtcg cgctggctgg tggccatcat gcggccggcg cggccgaaga acgggtcgct
      481 cgcgacgtcg ctggcggccg gcaggcccag gccgcgcagc agcgcgaggc cgcgctccag
      541 ccacgcgtcg cgccacgcct gcaccgcctc gggcgcaccg acgcggacga actcgcgcat
      601 gcggaaggac tgcaggcgtg tcggctcgtc cgagggctcg tgccgatagc accagttgcg
      661 cgtcgtgacg agccggccct gcgcgggcac gaggccgctg aacagcggat agaccggata
      721 gcaggccgcc ggcagcagca tggtctcggc gggctcgagc aggtgctccc agcgctgtcc
      781 cgcgtcggcg agcgccgcga gctcgcgcgc ggtcttctcg tcgccgaaga aactgtggat
      841 gctgcccacc agctgcggga agttgccgag gtagccgagg cgctcgatca gggcgcgcgc
      901 gacgatgggc gggaaatgca gcggctgcgc gccgtcgccg gcggtctcgc gcatgacgag
      961 ggcgtcgaag cgctcgagga tgtcctcgaa cttcgcggtg cggccgtcgc cgccgcgcac
     1021 gccggtcggc aggaggaacc cgtgctcgac caggccggcg cggaaggcgg cggggtcgta
     1081 cggatccggg gtctcgtgca ggctcattcc tggtcgtcct tgaacaccag cagcaggttg
     1141 gcgctctgcg cggcgatgcg gtcgttggag atcatcagcg cggccgacag cgcgtcgcgg
     1201 tagtggcggc cgaggctgaa cggcgtgtcg ttcttgtagc cgaggatgcc caccacctgc
     1261 agcgcgccgt gcacgacctc gggcgcgcgc tcggcggcgg cggtcttcag gctgttgaac
     1321 ttgagcgacc aggcgatgcg cgagagcggg tgcggctcgt ccgcggcggc gacggccgcg
     1381 ccggcccgag gcgcgaccgc ggtggacggc aggggcaggg cgtcgacctc cgcggcgacc
     1441 gactgccagt ggtggcgcat cgcctgcagg gtcgtcgtga gctcggccag ccgcagcgcc
     1501 gtgggcggcg tgctgcccgg gttgcggcgc gcctggccgc ggacgaaggc ggcggcgcgc
     1561 gcgtgggcgt cggtggcgat gcccgtccac agcgcggccc acaggacgtg cgaccagggc
     1621 accatcgtga gcgccgagat gtcggcgtag tccaccggca ggacctgcgc gcgcggcgcc
     1681 gacgactcga gccggaagcc cgggctgcag gtgccgcgca tgcccagcgt gtcccagctg
     1741 ccgacctggc gcagcgtcgc gtcggtcgcg cgcaggaacg cgagcacctg gtcgcccggc
     1801 gcggcctcgg cgtggcggcg ggcggtgacg aggatcgcgt ccgcatgcgc gcagtacgag
     1861 ccggtggtcg cgtccttggc gagcaggaag cgcgccgggt cgtccgcggg ttcgatggcg
     1921 cagatgctgg tgcgggtgtc gccgaaggtg ccggcctccg aggtcatcga cgcgaacagg
     1981 cgctgctcgg ccgccagctc gcgcagcacg gcctcgaact cggcgctgcc cgcggcgtgg
     2041 cgcgcgacgc aggcgacctg gatcgtgtgc atcgcgagca ccatcgcgct ggaggcgcag
     2101 ccctgcgcga gcgcatggca cagctgcccc agctgcgcga ggcccagccc cgcgccgccg
     2161 aggcgctcgg gcacggcggc cgacaggacg cgcgcggcac gcagcgcgtc gacggcctcc
     2221 tgcgggaagc gcgcctgggc atcgacgtcg gcggcggcgg gggccgcgat ctccttcgag
     2281 atgcggcgca cctcctgcag cagtcgggcg aagtccggat tgggcatggc cggtactgta
     2341 gccgcggggg cgggcggctt cccgcgtaat ccgaccagaa tatcggccgt gaacgacaag
     2401 gacgacgatt ccgtgcccga tgccacgacc cgcgccgggg tcgatacccc acagggtgtc
     2461 cagcccgacg tgacctggct cgagcctgcc ggccccgcgc gcgcggccgt gctgctgctg
     2521 cacggcctgg acatgacgcc cgcacagctc gcgccgctgc tgcgatcgct gcggctgccc
     2581 gcgtgggtcg cgctgccggc ggggccggtc gagcgcccgg gcgggc
//
LOCUS       DASVLN010000211        11739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_440791, whole genome shotgun sequence.
ACCESSION   DASVLN010000211 DASVLN010000000
VERSION     DASVLN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11739
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440791"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /gene="hscA"
                     /locus_tag="VF453_04765"
     CDS             complement(<1..1756)
                     /gene="hscA"
                     /locus_tag="VF453_04765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEX7686996.1"
                     /translation="MALLQISEPGQAPDPHQRRIAVGIDLGTTHSLVAAVRHGVAECL
                     PDDQGRVILPSVVRYLEGERRQVGHAAVEAQGEDAANTIVSAKRFMGRALADVAGHGR
                     LPYDFVDQPGMVGLRTRAGVKTAVEVSAEILATLRFRAEDTFDDEIHGAVITVPAYFD
                     DAQRQATKDAATLAGLNVLRLINEPTAAAIAYGLDQGVEGLYAVYDLGGGTFDVSLLR
                     LARGVFEVVATGGDSALGGDDYDQRLASALLARAGLPPLEQLAPAERRGVQAAARRAK
                     EVLSDAEHVEVAFEVAGTPRRMEVTRAELEAATAELTATTIRLLKRVLRDAKVGADEV
                     QGVVMVGGSTRMPAVRAAVGALFGRPPLTNLNPDEVVALGAAIQANALAGNAASGELL
                     LLDVTPLSLGLETMGGLVERIIERNSTIPTAKAQDFTTFKDGQTAMALHVVQGERELV
                     SDCRSLARFELRGIPPMVAGAARIRVTFQVDADGLLSVSAREQGSGVEAAIVVKPSYG
                     LGDEQVAAMLREGFASAESDMARRSLREAQVEAQRMILATESALAADGDLLAADERTA
                     IDALLARVREAAATAAEPR"
     gene            complement(1808..2353)
                     /gene="hscB"
                     /locus_tag="VF453_04770"
     CDS             complement(1808..2353)
                     /gene="hscB"
                     /locus_tag="VF453_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEX7686997.1"
                     /translation="MDDRLTALDLSATDFALFGLPERFGLDLAALDATWKALQGAAHP
                     DRHASASPSAQRLAMQWAVRINEAYRRLKDPLSRAAYLCTLHGVDVAAESNTAMPAAF
                     LMQQMEWREALEDARDADAVEALADAVAAQRRATLEQLRARIDEAGGVDWPAVAQTVR
                     GLMFVDRFLADVDRRLEALQD"
     gene            complement(2338..2823)
                     /locus_tag="VF453_04775"
     CDS             complement(2338..2823)
                     /locus_tag="VF453_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7686998.1"
                     /translation="MNLTIRPTPPDHPRVIALIEQLDAYLYSVYPPDEVYALDVESLK
                     HPTVTFLGVWDGEDAVGCGAVRVMDGDAGTGGARYGEIKRMFVSPARRGQGIARRVLA
                     ALEDHLRGQGIDRALLETGDPQVEAVRLYERTGYLRRGPFGDYPDNGSSVFMEKRWTT
                     A"
     gene            complement(2960..3283)
                     /gene="iscA"
                     /locus_tag="VF453_04780"
     CDS             complement(2960..3283)
                     /gene="iscA"
                     /locus_tag="VF453_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEX7686999.1"
                     /translation="MAVTLTEAAARHVTRYLSKRGRGVGVRLGVKTTGCSGLAYKLEY
                     ADDFAPEDIVFEGHGVKVLVDPKSLPYIDGTELDFVREGLNEGFKFHNPREKDRCGCG
                     ESFRV"
     gene            complement(3295..3726)
                     /gene="iscU"
                     /locus_tag="VF453_04785"
     CDS             complement(3295..3726)
                     /gene="iscU"
                     /locus_tag="VF453_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEX7687000.1"
                     /translation="MAYSNKVIDHYENPRNVGGFDKGDETVGTGMVGAPACGDVMKLQ
                     IKVDPATGLIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEALSIRNTQIAEELALPP
                     VKIHCSILAEDAIKAAVNDYRTRHGAAAPQAAASETAPANA"
     gene            complement(3800..5014)
                     /locus_tag="VF453_04790"
     CDS             complement(3800..5014)
                     /locus_tag="VF453_04790"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX7687001.1"
                     /translation="MTPHLPIYMDYGATTPVDPRVVDAMIPWLREHFGNAASRSHAWG
                     WEAEEAVERARKQVADLIGADPREIVWTSGATESNNLALKGAAQFYKTRGKHLITVKT
                     EHKCVLDTMRELERQGFEVTYLDVLESGLLDLDAFKAAIRPDTILASVMFVNNEIGVI
                     QDVESIGRICRERGMIFHVDAAQATGKVDIDLSKLPIDLMSLASHKTYGPKGIGALYV
                     RRKPRVRIEPQMHGGGHERGMRSGTLPTHQIVGMGEAFRIAGEEMGAERERIRALQKR
                     LYDGLAGIDQVFVNGDLERRVPHNLNLSFNFVEGESLIMGVKGLAVSSGSACTSASLE
                     PSYVLRALGRSDELAHSSLRMTIGRFTTEEEIDSAVRMLKDTVARLRELSPLWDMYRE
                     GIDLNSIQWAAH"
     gene            complement(5069..5602)
                     /gene="iscR"
                     /locus_tag="VF453_04795"
     CDS             complement(5069..5602)
                     /gene="iscR"
                     /locus_tag="VF453_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEX7687002.1"
                     /translation="MRLTTKGRFAVTAMIDLALRSSNGPVALAAISQRQQISLSYLEQ
                     LFGKLRRHELVESTRGPGGGYSLGRGAESITVADIIVAVDEPIDATGCAGKENCMGDD
                     TGRCMTHDLWASLNVKMIEFLDSISLKKLVDEQLAKGVSIEEVPVRRAISTQPVVKPI
                     KVNAPNSVFALGNAFSK"
     gene            complement(5921..6436)
                     /locus_tag="VF453_04800"
     CDS             complement(5921..6436)
                     /locus_tag="VF453_04800"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HEX7687003.1"
                     /translation="MSPESAPAPDHAAAGGRIRVLMVCLGNICRSPTAEAMLRRHVRE
                     AGLEALVEVDSAGTADYHVGSPPDRRAIAHGEGRGLAMRHLRGRQVERADFDRFDFVF
                     AMDEDNLANLARLRPAGSRARLGLLLDLAPHAGAREVPDPYYGGAEGFERVLDLVDAA
                     CAAFVATLGRG"
     gene            complement(6433..8577)
                     /gene="uvrB"
                     /locus_tag="VF453_04805"
     CDS             complement(6433..8577)
                     /gene="uvrB"
                     /locus_tag="VF453_04805"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX7687004.1"
                     /translation="MPDEPSPAALAHASSSGEPQEPPKGEFVSFPDSPFQLFQPYPPA
                     GDQPTAIAGLCEGIEDGLTYQTLLGVTGSGKTYTMANVIARMGRPAIVFAPNKTLAAQ
                     LYSEFREFFPKNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSV
                     LERRDTIIVASVSAIYGIGKPEDYTQMRFILRNGDKLGQREVIAQLIRMQYTRNETDF
                     SRGSFRVRGDTIDVFPAEHSELAVRIELFDDEVESLQLMDPLTGQVRQKVPRFTIYPS
                     SHYVTPRERVLAAVEGIKEELRWRLDDFVKAGKLVEAQRLEQRTRFDLEMLQEVGHCK
                     GIENYTRHLSGSKPGEPPPTLVDYLPKDAIMFIDESHVMMGQIGGMFNGDRARKQTLV
                     DYGFRLPSALDNRPLKFEEFERKMRQCVFVTATPADYEKQHAGQVVEQLVRPTGLVDP
                     VVEVRPATTQVDDVLQEIRERTDAGDRVLVTTLTKKMAEQLTEYLTENGVKVRYLHSD
                     IDTVERVEILRDLRLGTFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRAERSLIQ
                     TIGRAARNVNGMAILYADRMTDSMRRAIDETERRRAKQMAHNAAMGITPKGIRKTIRD
                     LIDGVAAERSGKDDLKFAQQAAEVEAMSEKDLGKRIKALEKQMLEHAKSLEFEKAARV
                     RDQLALLREQAFGGGGAHDANVAVPAGAARRA"
     gene            8772..9971
                     /locus_tag="VF453_04810"
     CDS             8772..9971
                     /locus_tag="VF453_04810"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEX7687005.1"
                     /translation="MSLFAAVELAPRDPILGLNEQFNADPNPAKVNLGVGVYTDENGK
                     LPLLGCVAAAEKQLVDAQKARGYLPIDGIAAYDKAVQGLVFGADSAVVKDGRVATVQA
                     LGGTGGLKVGADFLKRLRPDATVLISDPSWENHRALFTQAGFPVETYPYYDAAKRGIA
                     FDALVAKLEASPAGTVVVLHACCHNPTGYDLAPAQWDRIIAVVKARGLVPFLDMAYQG
                     FGSGLAEDGAVVQQFLAAGLDFFVSTSFSKSFSLYGERVGALSVVCASKDEASRVLSQ
                     LKIMIRTNYSNPPTHGAQVVATVLTTPALRAQWEQELAGMRDRIKRMRRALVERLAAA
                     GVKGDLGFIVEQNGMFSYSGLSAAQMQRLRSEFGVYGVDSGRICVAALNEHNLAYVAK
                     AIAAVSA"
     gene            10312..>11739
                     /gene="phaZ"
                     /locus_tag="VF453_04815"
     CDS             10312..>11739
                     /gene="phaZ"
                     /locus_tag="VF453_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX7687006.1"
                     /translation="MLYQLYETQRALMAPFADFAEATAKLYKHPLSPFTHVPGAHRLA
                     AAFELVHRLGMDYEKPAFDIQTVASGGVKVAVQEQVAIEKPFCRLLRFKRFTDDAATL
                     ERMRAQPTVLVVAPLSGHHATLLRDTVRSLLQDHKVYITDWTDARMVPVEAGPFHLDD
                     YIRYVQEFVRHIGADVHVISVCQPTVPVLAAISLMASAGEATPLSMTMMGGPIDARKS
                     PTAVNNLAMNRSYEWFENNVIYRVPQNYPGSQRQVYPGFLQHTGFVAMNPDRHASSHY
                     DYFLDLVRGDEDSAEAHRKFYDEYNAVLDLPAEYYLDTIRTVFQDFALVNGSWEVDGK
                     LVRPQDIEDSALLTVEGELDDISGAGQTRAAHGLCTGIPATRQFHYDVPGAGHYGIFS
                     GRRWRELVYPQVRDFIARFEKERQGDRAVTPAVKAQATRAVRKAKASVPAAAPATEVK
                     ATTPARKVRHTATKVAPASAGVPVAG"
BASE COUNT         1707 a         4106 c         4094 g         1832 t
ORIGIN      
        1 cgcgcggctc ggcggcggtg gcggccgcct cgcgcacgcg cgcgagcagc gcgtcgatcg
       61 cggttcgctc gtcggcggcg aggaggtcgc cgtcggccgc cagcgcggac tcggtggcga
      121 ggatcatgcg ctgggcctcg acctgcgcct cgcgcaggct gcggcgggcc atgtcggact
      181 cggcggacgc gaagccttcc ctgagcatcg cggcgacctg ctcgtcgccc aggccgtagc
      241 tgggcttgac gacgatggcc gcctcgacgc ccgagccctg ctcgcgcgcc gacacgctca
      301 gcaacccgtc ggcgtcgacc tggaaggtga cgcggatgcg cgccgcgccg gcgaccatcg
      361 gcgggatgcc gcgcagctcg aagcgcgcga gcgagcggca gtcggagacc agctcgcgct
      421 cgccctgcac cacgtgcagc gccatcgcgg tctggccgtc cttgaaggtg gtgaagtcct
      481 gcgccttggc ggtcgggatc gtcgagttgc gctcgatgat gcgctcgacc agtccgccca
      541 tcgtctccaa tcccagcgac agcggcgtca cgtccagcag cagcagctcg ccgctggcgg
      601 cgttcccggc cagggcgttg gcctggatcg cggcacccag cgcgacgacc tcgtccgggt
      661 tcaggttggt cagcggcggg cggccgaaga gcgcgccgac ggcggcgcgc acggccggca
      721 tgcgcgtcga gccgccgacc atcacgacgc cctggacctc gtccgcgccg accttggcgt
      781 cgcgcaacac gcgcttgagc aggcggatcg tcgtcgccgt gagctcggcg gtcgcggcct
      841 cgagctcggc acgcgtgacc tccatacgcc ggggggtacc ggcgacttca aacgcgacct
      901 cgacgtgttc ggcgtcgctc aagacctcct tggcacggcg cgcggccgcc tgcacgccgc
      961 ggcgctcggc gggagcgagc tgctccagcg gcggcaggcc ggcacgggcg agcaacgcgc
     1021 tcgcgaggcg ctggtcgtag tcgtcgccgc ccagggccga gtcgccaccc gtggccacga
     1081 cctcgaacac accgcgcgcc aggcgcagca gcgagacgtc gaaggtgccg ccgcccaggt
     1141 cgtagaccgc gtacaggcct tcgacgccct ggtcgaggcc gtaggcgatc gccgcggcgg
     1201 tgggctcgtt gatcaggcgc aggacgttca ggccggcgag cgtcgcggcg tccttggtcg
     1261 cctggcgctg ggcgtcgtcg aaataggccg gcacggtgat gaccgcgccg tggatctcgt
     1321 cgtcgaaggt gtcctcggcg cggaagcgca gcgtcgccag gatctcggcc gagacctcca
     1381 ccgccgtctt cacgcccgcg cgcgtgcgca ggccgaccat gcccggctgg tcgacgaagt
     1441 cgtacggcag ccggccgtgg cccgcgacgt ccgccagcgc gcgccccatg aagcgcttgg
     1501 ccgagacgat ggtgttggcg gcgtcctcgc cctgggcctc gaccgcggcg tggccgacct
     1561 ggcgacgctc gccctcgagg tagcgcacga ccgagggcag gatcacgcgg ccctggtcgt
     1621 cgggcaggca ctcggccacg ccgtggcgca ccgcggccac gagcgagtgc gtcgtgccga
     1681 ggtcgatgcc gacggcgatg cggcgctggt gcgggtcggg cgcctggccg ggttcggaga
     1741 tctggagcag agccatggtg gaaacgggaa gcgtgtctct tcgttcttgt cggaatcggg
     1801 gcaggcgtca gtcctgcagc gcctcgaggc ggcggtcgac gtcggccagg aagcggtcga
     1861 cgaacatcag cccgcgcacc gtctgcgcga cggcgggcca gtcgaccccg ccggcctcgt
     1921 cgatgcgcgc gcgcagctgc tcgagcgtcg cccggcgctg cgccgcgacg gcgtcggcga
     1981 gcgcctcgac ggcgtcggcg tcgcgggcat cctcgagcgc ctcgcgccat tccatctgct
     2041 gcatcaggaa cgccgcgggc atcgcggtgt tgctctcggc ggcgacatcg accccgtgca
     2101 gcgtgcagag gtaggccgcg cgggagagcg gatccttgag gcggcggtag gcctcgttga
     2161 tgcggacggc ccactgcatc gccaggcgct gcgcggacgg cgacgcgctc gcgtggcggt
     2221 cgggatgcgc agcgccctgc agcgccttcc aggtcgcgtc gagcgcggcg agatcgaggc
     2281 cgaagcgctc gggcaggccg aacagcgcga agtcggtcgc ggagagatcg agcgcggtca
     2341 ggcggtcgtc catcgcttct ccatgaacac ggacgaaccg ttgtccgggt agtcgccgaa
     2401 cgggccgcgg cgcaggtatc cggtgcgctc gtacagccgc accgcctcga cctgggggtc
     2461 gccggtctcg agcagcgccc ggtcgatgcc ctggccgcgc agatgatcct cgagcgccgc
     2521 cagcacccgg cgggcgatgc cctggccgcg ccgcgccggc gagacgaaca tgcgcttgat
     2581 ctcgccgtag cgcgcgccgc cggtgccggc gtcaccgtcc atcacgcgca ccgcgccgca
     2641 gccgacggcg tcctcgccgt cccagacgcc gaggaaggtc accgtcgggt gcttgagcga
     2701 ctcgacgtcg agcgcgtaga cctcgtcggg cggatagacc gagtacaggt aggcgtcgag
     2761 ctgctcgatc agcgcgatga cgcggggatg gtcgggaggc gtggggcgaa tggtgaggtt
     2821 catggtggcg gggcaagcac gattcacgcc gcgcgcggcc gccgacgcgg tgcaccggca
     2881 cgcgcggatc ggccgcgccg gctcaggaac gaaaaggcgc gacctcggcc gcgcccccgt
     2941 gcctgtcgcg gcaggcggtt cagacgcgga aggactcgcc gcagccgcag cgatccttct
     3001 cgcgcgggtt gtggaacttg aagccctcgt tgaggccctc gcgcacgaag tccagctccg
     3061 tgccgtcgat gtagggcagg ctcttcgggt cgacgagcac cttcacgcca tggccctcga
     3121 agacgatgtc ctcgggggcg aaatcgtccg cgtattcgag cttgtaggcg aggccggagc
     3181 agccggtcgt cttcacgccc agccgcacac ccacgccgcg tccgcgcttg ctgaggtagc
     3241 gcgtcacgtg ccgggccgcc gcttcggtca gggtcaccgc catcgcgtcg ggcctcaggc
     3301 gttggccggg gccgtctcgc tggccgcggc ctgcggcgcg gcggcgccgt ggcgcgtgcg
     3361 gtagtcgttg acggcggcct tgatcgcgtc ctcggccagg atcgagcaat ggatcttcac
     3421 cggcggcagc gccagttcct cggcgatctg cgtgttgcgg atggacagcg cctcgtcgag
     3481 cgacttgccc ttcacccact cggtcacgag cgacgacgac gcgatcgccg agccgcagcc
     3541 gtaggtcttg aagcgcgcgt cctcgatgag cccggtcgcc gggtcgacct tgatctgcag
     3601 cttcatcacg tcgccgcagg ccggcgcgcc gaccatgccg gtgccgaccg tctcgtcgcc
     3661 cttgtcgaat ccgccgacgt tgcgcgggtt ctcgtagtgg tcgatgacct tgttgctgta
     3721 agccatgatg tctaccctcg gatgtcttcg atgatgttgc aggtgcggcg cgtcgattgc
     3781 ggccgtcggg gaggccgcgt cagtgcgccg cccactggat cgagttgagg tcgatgccct
     3841 cgcggtacat gtcccacagc ggggacagct cgcgcaggcg cgcgaccgtg tccttgagca
     3901 tgcgcaccgc ggagtcgatc tcctcctcgg tggtgaaccg gccgatcgtc atgcgcagcg
     3961 acgagtgcgc cagctcgtcg ctgcggccga gcgcgcgcag cacgtagctc ggctccaggc
     4021 tcgcggacgt gcaagccgaa ccggaggaca ccgcgaggcc cttgacgccc atgatgagcg
     4081 actccccctc gacgaagttg aacgacaggt tcaggttgtg cggcactcgg cgctcgaggt
     4141 cgccgttgac gaagacctgg tcgatgcccg cgaggccgtc gtagagccgc ttctgcagcg
     4201 cgcggatgcg ctcgcgctcg gcgcccatct cctcgccggc gatgcggaag gcctcgccca
     4261 tgcccacgat ctggtgcgtg ggcagcgtgc cggagcgcat gccgcgctcg tggccgccgc
     4321 cgtgcatctg cggctcgatg cgcacgcgcg gcttgcggcg cacgtacagc gcgccgatgc
     4381 ccttcgggcc gtacgtcttg tgcgatgcca ggctcatcag gtcgatcggc agcttcgaga
     4441 ggtcgatgtc gaccttgccc gtcgcctgcg ccgcgtcgac gtggaagatc atgccgcgct
     4501 cgcggcagat ccggccgatc gactcgacgt cctggatcac gccgatctcg ttgttcacga
     4561 acatgaccga tgcgaggatc gtgtccgggc ggatcgccgc cttgaacgca tcgaggtcca
     4621 gcaggccgct ctccagcacg tcgaggtagg tgacctcgaa gccctggcgc tcgagctcgc
     4681 gcatcgtgtc gagcacgcac ttgtgctccg tcttgaccgt gatcaggtgc ttcccgcggg
     4741 tcttgtagaa ctgcgcggcg cccttcagcg cgaggttgtt cgactcggtc gccccggacg
     4801 tccacacgat ttcccgcgga tcggcgccga tcaggtcggc gacctgcttg cgtgcgcgct
     4861 ccaccgcctc ctcggcctcc cagccccacg cgtgcgaacg cgacgcggcg ttgccgaagt
     4921 gctcgcgaag ccaggggatc atggcgtcga cgacgcgcgg gtcgacgggc gtcgtcgcgc
     4981 cgtagtccat gtagatcggg agatgcgggg tcatggggca ggaagtcgtg ggatctggaa
     5041 aaggaacggg cgcgtcagga cgacgcggtc acttcgagaa cgcgttgccg agcgcgaaca
     5101 cggagttcgg cgcgttgacc ttgatgggct tgacgaccgg ctgcgtcgag atggcccggc
     5161 gcacgggcac ttcctcgatc gacacgccct tcgcgagctg ctcgtcgacg agcttcttca
     5221 gcgagatgga gtcgaggaac tcgatcatct tcacgttgag gctggcccac aggtcgtgcg
     5281 tcatgcagcg accggtgtcg tcacccatgc agttctcctt gccggcgcag cccgtggcgt
     5341 cgatcggctc gtcgacggcg acgatgatgt cggccaccgt gatcgactcg gcgccgcggc
     5401 ccagcgagta gccgccaccg gggccgcgcg tgctctcgac aagctcgtgg cggcgcagct
     5461 tgccgaacag ctgctcgagg taggacaggg agatctgctg gcgctgcgag atcgccgcca
     5521 gggccaccgg accattgctg gagcggagag ccagatcgat catcgcagtg acggcaaatc
     5581 ggcctttggt ggtcagtcgc atggggtctc tcctgaggca attgaggcca ccccacacgg
     5641 ggtaaccctg ggttttcaac gtgacttgat tccgagcgtt ttagtcgatt atgccagaag
     5701 cctaccgatt ttgtcgagat tgaccctgga gtcaatgccc gaggccggac gacctcggcg
     5761 gccgggtgcg cgcttgttgc gcactgcagc acgacgcccc gcgactgtaa cgactaggga
     5821 aagcccgcac cgccaacgtc gcgataccag cggggtcaag ggatcacgta gcgataccga
     5881 aagactcgga tactcgacgc agttgctcgg cttcagctgc tcagccgcgg cccagtgtcg
     5941 cgacgaaggc ggcgcatgcc gcgtcgacga ggtcgagcac gcgctcgaag ccttccgcac
     6001 cgccgtagta ggggtcgggc acctcgcggg cgccggcgtg cggcgcgagg tccagcagca
     6061 gcccgaggcg ggcgcgcgat ccggccgggc gcaggcgtgc caggttcgcg aggttgtcct
     6121 cgtccatcgc gaagacgaag tcgaagcggt cgaaatccgc gcgttcgacc tgacggccgc
     6181 gcaggtgccg catcgccagg ccgcggccct cgccgtgcgc gatcgcgcgc cgatccggcg
     6241 gcgagcccac gtggtagtcc gcggtgccgg cggagtcgac ctcgaccagc gcctccagcc
     6301 ccgcctcccg cacgtgccgg cgcagcatgg cctcggccgt cggcgaacgg cagatgttgc
     6361 cgaggcagac catgaggacg cggatgcggc cgccggctgc ggcgtgatcg ggcgcgggag
     6421 cgctctccgg gctcatgcgc ggcgcgccgc gcccgccggc accgcgacgt tcgcgtcgtg
     6481 cgcgcccccg ccgccgaagg cctgctcccg cagcagcgcg agctggtcgc gcacgcgcgc
     6541 ggccttctcg aactcgaggc tcttggcgtg ctcgagcatc tgcttctcga gcgccttgat
     6601 gcgcttgccg agatccttct cgctcatcgc ctcgacttcg gcggcctgct gcgcgaactt
     6661 caggtcgtcc ttgccggaac gctccgcggc cacgccgtcg atcaggtcgc ggatggtctt
     6721 gcggataccc ttcggggtaa tgcccatcgc ggcgttgtgc gccatctgct tcgcgcgccg
     6781 gcgctcggtc tcgtcgatgg cccgccgcat cgagtcggtc atcctgtcgg catacaggat
     6841 cgccatgccg ttgacgttgc gcgccgcgcg gccgatggtc tggatgaggc tgcgctcggc
     6901 gcgcaggaag ccctccttgt cggcatccag gatcgccacc agcgagacct cggggatgtc
     6961 caggccctcg cgcagcaggt tgatgcccac cagcacgtcg aaggtgccca ggcgcaggtc
     7021 gcgcaggatc tcgacgcgct cgacggtgtc gatgtcggag tgcaggtagc gcaccttcac
     7081 gccgttctcg gtcaggtact cggtcagctg ctcggccatc ttcttggtga gcgtcgtcac
     7141 gagcacgcgg tcgccggcgt ccgtgcgctc gcggatctcc tgcagcacgt cgtcgacctg
     7201 cgtcgtcgcg ggacgcacct cgaccacggg gtccaccagg ccggtgggac gcacgagctg
     7261 ctcgacgacc tgtccggcat gctgcttctc gtagtccgcg ggcgtggccg tgacgaacac
     7321 gcactggcgc atcttgcgct cgaactcctc gaacttcagc ggccggttgt ccagcgccga
     7381 cggcaggcgg aagccgtagt cgaccagcgt ctgcttgcgc gcgcggtcgc cgttgaacat
     7441 gccgccgatc tggcccatca tcacgtggct ctcgtcgatg aacatgatcg cgtccttggg
     7501 caggtagtcg accagcgtgg gcggcggctc gccgggcttc gagccggaca ggtggcgcgt
     7561 gtagttctcg atgcccttgc agtggccgac ctcctgcagc atctcgaggt cgaagcgcgt
     7621 gcgctgctcg agccgctgcg cctccacgag cttgccggcc ttgacgaagt cgtcgaggcg
     7681 ccagcgcagc tcctccttga tgccttcgac cgccgccagc acgcgctcgc gcggcgtgac
     7741 gtagtggctc gagggataga tcgtgaagcg cggcaccttc tggcgcacct ggccggtgag
     7801 cggatccatc agctgcagcg actcgacctc gtcgtcgaac agctcgatgc gcacggcgag
     7861 ctccgagtgc tccgccggga agacgtcgat cgtgtcgccg cgcacgcgga agctgccgcg
     7921 cgagaagtcg gtctcgttgc gcgtgtactg catgcggatc agctgcgcga tgacctcgcg
     7981 ctgaccgagc ttgtcgccgt tgcgcaggat gaagcgcatc tgcgtgtagt cctcgggctt
     8041 gccgatgccg tagatcgccg acacgctcgc cacgatgatg gtgtcgcggc gctcgagcac
     8101 gctcttggtc gccgacagcc gcatctgctc gatgtgctcg ttgatcgacg agtccttctc
     8161 gatgaacagg tcgcgctgcg ggacgtaggc ctcgggctgg tagtagtcgt agtagctgac
     8221 gaagtactcg acggcgttct tcgggaagaa ctcgcggaat tcgctgtaca gctgcgcggc
     8281 gagcgtcttg ttgggcgcga agacgatcgc cggacgcccc atgcgggcga tgacgttggc
     8341 catcgtgtag gtcttgcccg agcccgtgac gcccagcagc gtctggtagg tcaggccgtc
     8401 ctcgatgccc tcgcacaggc ccgcgatcgc ggtcggctgg tcgccggcgg gcgggtacgg
     8461 ctggaacagc tggaacggcg agtccgggaa actgacgaac tcacccttcg gcggttcctg
     8521 cggctcgccg ctcgacgacg cgtgggccag cgcggcgggc gacggctcgt ccggcatgac
     8581 ctcttgacga ttcggcatgg ggtattccct cagaaggcgc ttaaaatcga cgcgctgcag
     8641 ctccgcacca cgagtgcgcc gacgaacctg acagcgtagc gcaccccgga cgccattcgc
     8701 gtcgcgccgg ccgcgcgacc cccgcaactg ccgtcgttca ccggcttttc aagccacatc
     8761 ctggaagctc catgtccctg tttgccgccg tcgaactcgc cccgcgagac ccgatcctgg
     8821 gcctgaacga acagttcaac gccgacccca atcccgccaa ggtcaacctg ggcgtgggcg
     8881 tgtacaccga cgagaacggc aagctgccgc tgctgggctg cgtggccgcg gccgagaagc
     8941 agctcgtcga cgcccagaag gcgcgcggct acctgcccat cgacggcatc gccgcgtatg
     9001 acaaggccgt gcagggcctc gtgttcggcg cggacagcgc ggtggtgaag gacggccgcg
     9061 tggcgaccgt gcaggcgctc ggcggcaccg gcggcctgaa ggtcggcgcg gacttcctga
     9121 agcgcctgcg cccggacgcg accgtgctga tcagcgaccc gagctgggag aaccaccgcg
     9181 cgctgttcac gcaggccggc ttcccggtcg agacctaccc gtactacgac gccgcgaagc
     9241 gtggcatcgc cttcgacgcg ctggtcgcga agctcgaggc ctcgcccgcc ggcacggtcg
     9301 tcgtgctgca cgcctgctgc cacaacccga ccggctacga ccttgcgccg gcgcagtggg
     9361 acaggatcat cgccgtcgtg aaggcgcgcg gcctcgtgcc cttcctcgac atggcctacc
     9421 agggcttcgg ctcgggcctg gccgaggacg gcgcggtcgt gcagcagttc ctcgccgccg
     9481 gcctggactt cttcgtctcg acctccttct cgaagagctt ctcgctctac ggcgaacgcg
     9541 tcggcgcgct gagcgtcgtc tgcgcgagca aggacgaggc gtcgcgcgtg ctgtcgcagc
     9601 tgaagatcat gatccgcacg aactactcca acccgccgac gcacggcgcg caggtggtcg
     9661 cgaccgtgct gacgacgccc gcgctgcgcg cccagtggga acaggagctc gccggcatgc
     9721 gcgaccgcat caagcgcatg cgccgcgcgc tggtcgagcg gctcgccgcg gccggcgtga
     9781 agggcgacct cggcttcatc gtcgagcaga acggcatgtt cagctactcg ggcctgtctg
     9841 cggcccagat gcagcgcctg cgctcggagt tcggcgtcta cggcgtcgac tccggccgca
     9901 tctgcgtcgc cgcgctgaac gagcacaacc tggcctacgt cgccaaggcg atcgcggcgg
     9961 tctcggcctg agcgaagcgc cttcgcccca tccccagccc cgacgcgatc gcctcggggt
    10021 tttttttcgc cactcggcga ccgcacccga cgggatcggg ccatggcgcc ttctccgaca
    10081 cggaaaccgc cgctgttccg tcgatcgacg caccgctggc ggcgcatagt cgaggcgggc
    10141 gtcgcttttg cgcccggggc aagcatgcgc cgcggaatcg gcgtactatt cctcggattg
    10201 ctgcgccgca gcaattaagc cgccgaacga aatcgtcaag aatccgttca gctcgacggc
    10261 acaatcgctg ctcaaccgtc ccgtcgcgtc cgaaaggcca ggtttcccgt catgctgtac
    10321 caactctacg aaacccagcg cgcgctgatg gcgccgttcg ccgatttcgc cgaggcgacc
    10381 gccaagctct acaagcaccc gctgtcgccg ttcacgcacg tccccggcgc gcaccgcctg
    10441 gccgcggcct tcgaactggt gcaccggctc ggcatggact acgagaagcc ggcgttcgac
    10501 atccagaccg tggccagcgg cggcgtcaag gtcgccgtgc aggagcaggt cgccatcgag
    10561 aagccgttct gccgcctgct gcgcttcaag cgcttcaccg acgatgccgc cacgctcgag
    10621 cgcatgcggg cgcagccgac ggtgctggtc gtggcgccgc tgtccggtca ccacgcgacg
    10681 ctgctgcgcg acaccgtgcg cagcctgctg caggaccaca aggtctacat caccgactgg
    10741 acggacgcgc gcatggtgcc ggtcgaggcc ggcccgttcc acctcgacga ctacatccgc
    10801 tacgtgcagg agttcgtccg ccacatcggg gccgacgtgc acgtgatctc ggtgtgccag
    10861 ccgacggtgc cggtgctcgc ggcgatctcg ctcatggcca gcgcgggcga ggccacgccg
    10921 ctgagcatga cgatgatggg cggcccgatc gatgcccgca agtcgcccac cgccgtgaac
    10981 aacctggcaa tgaaccgcag ctacgagtgg ttcgagaaca acgtgatcta ccgcgtgccg
    11041 cagaactacc ccggctcgca gcggcaggtc tacccgggct tcctgcagca caccggcttc
    11101 gtggcgatga atcccgaccg ccacgcctcc tcgcactacg actacttcct cgacctggtg
    11161 cgcggcgacg aggactccgc cgaggcgcac cgcaagttct acgacgagta caacgccgtg
    11221 ctcgacctgc cggccgagta ctacctcgac acgatccgga ccgtgttcca ggacttcgcg
    11281 ctcgtcaacg gcagctggga agtcgatggc aagctggtgc gcccgcagga catcgaggac
    11341 agcgcgctgc tgacggtcga gggtgaactc gacgacatct cgggcgcggg ccagacgcgc
    11401 gcggcccacg ggctgtgcac cggcatcccc gccacgcgcc agttccacta cgacgtgccc
    11461 ggcgctggcc actacggcat cttctccggc cggcgctggc gcgagctggt ctacccgcag
    11521 gtgcgcgact tcatcgcgcg cttcgagaag gagcgccagg gcgaccgcgc ggtcaccccg
    11581 gccgtgaagg cgcaggccac gcgcgcggtc aggaaggcca aggcgagcgt gccggccgcg
    11641 gcgcccgcga ccgaggtcaa ggcgacgacg ccggccagga aggtgcgcca cacggccacc
    11701 aaggttgcgc cggcgagcgc gggcgtgccg gtggccggc
//
LOCUS       DASVLN010000212         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_279684, whole genome shotgun sequence.
ACCESSION   DASVLN010000212 DASVLN010000000
VERSION     DASVLN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279684"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..707)
                     /locus_tag="VF453_04820"
     CDS             complement(186..707)
                     /locus_tag="VF453_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687007.1"
                     /translation="MRRCVARGLALASLAAAAAMPAQAVEPLRQLDIVLLEPGAVIEQ
                     RVDGGADGLADYVKRLGEAAAETMRAHPRQLPDAGFIVVAARPGGQSRTWFDFAVPLM
                     PETATALSEAIAAVRPTPVARDEIVFALRVSLWGGRPSARVAPVPPEWREAGRQAGRR
                     LAVDELVDRLWPR"
     gene            885..1244
                     /locus_tag="VF453_04825"
     CDS             885..1244
                     /locus_tag="VF453_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HEX7687008.1"
                     /translation="MRSSNPNGGLVYSTDAGRMCPGCRRPVAQCACRQAAPPAGDGVV
                     RVFFETKGRGGKGVTVVRGLGLDDAGLAPIAKQLKAACGVGGTVKDGAIELQGEHRDR
                     VVALLQSQGRTVRKAGG"
     gene            1244..1645
                     /locus_tag="VF453_04830"
     CDS             1244..1645
                     /locus_tag="VF453_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687009.1"
                     /translation="MGAHLEPGRASPAGHPRRLGAPFSGALRSFSFWIANGTLGLPLL
                     EGIDYWPALRESPSLLEETYAIFANVLEFDATGTPVNAKYAEYRAAQCIRRWCDPAYE
                     VVPPFEDWETALHAPPSRSDAKPWPPGVPRP"
     gene            1718..>2343
                     /locus_tag="VF453_04835"
     CDS             1718..>2343
                     /locus_tag="VF453_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687010.1"
                     /translation="MSSPHTTAALHRLRPLALAAALAGALVGFGPARATDALSAAIPS
                     DLPDARLDFGDRHVGLPAVGRWTLVQREERPLAERRDKGSVHETWAVAMDGPRVRMVV
                     RLMMPSAGAHRTRRGVLSPCLEPEGILREDLSSRAGLPECLAIFGHENLRGIFRRRAQ
                     PTASWIAAHAEVDPSAPAVEFAYTKRADTAFGSVRIFTTTDAFPADDDA"
BASE COUNT          299 a          891 c          869 g          284 t
ORIGIN      
        1 gttcgcagga cgcaagcaag tcgtgatcga cgcgccgggc gtcgccgcgg ccgatgcatg
       61 cgcgaccgca ggtgttcgcc gatcgcctgt cgtccgtcgc cgttggccga tcgccgacgg
      121 gcgtccgcgc accccgcgcc gcctcggtgc gtggtgccgg cggggcgagt cgttcccgga
      181 actcgtcagc gtggccagag gcgatcgacc agctcgtcca cggccagccg ccgcccggcc
      241 tggcggcccg cctcgcgcca ttcgggcggc acgggcgcga ccctggcgct cggccggccg
      301 ccccacaggc tgacgcgcag ggcgaacacg atctcgtcgc gcgccacggg cgtcggccgg
      361 acggcggcga tggcctcgga cagcgcggtc gccgtttcgg gcatcagcgg cacggcgaag
      421 tcgaaccagg tgcgcgactg gccgccgggc cgcgccgcga cgacgatgaa gcccgcgtcg
      481 ggcagttgcc gcggatgcgc gcgcatcgtc tccgcggccg cctcgcccag tcgcttgacg
      541 tagtcggcga ggccgtccgc accgccatcg acgcgctgct cgatgaccgc gcccggctcc
      601 agcagcacga tgtcgagctg tcgaagcggc tcgaccgcct gcgccggcat cgcggcggcg
      661 gcggccaggg acgcgagcgc gagccccctg gcgacgcaac gacgcaaggg cgcgcgatcg
      721 aggcagcgag gacgaagggg cgacggcatg gcaggacgga ccggggcagg gcgcgcgggc
      781 gccgatgcgg gattgtcaca tcgggcgagc gccgggcagt gggccgaccg ccggcggccc
      841 gggcttcgtc gacgccgcgc ggcgcgatcg gttccaatac ggccatgcga tcctcgaatc
      901 ccaacggcgg cctcgtctac tcgaccgacg ccggccgcat gtgccccggc tgtcgccgtc
      961 ccgtcgccca atgcgcctgt cgacaggccg cgccgcccgc gggcgacggc gtcgtgcgcg
     1021 tcttcttcga gaccaagggg cgcggcggca agggcgtgac ggtggtgcgc gggctgggcc
     1081 tcgacgacgc cgggctcgcg ccgatcgcca agcagctgaa ggccgcctgc ggcgtcggcg
     1141 gcaccgtgaa ggacggcgcg atcgagctgc agggcgagca ccgcgaccgc gtcgtggcgc
     1201 tgctgcagtc gcaggggcgg acggtcagga aggcgggcgg gtgatgggcg cccacctcga
     1261 accgggccgc gcatcaccgg ccgggcaccc gcggcgactg ggcgcgccgt tctccggcgc
     1321 gttgcgcagc ttcagcttct ggatcgcgaa cggcacgctc ggcctgccgc tgctggaagg
     1381 catcgactac tggcccgcgc tgcgcgagtc gccgagcctc ctcgaggaga cgtacgcgat
     1441 cttcgccaac gtcctggaat tcgatgccac cggcacgccg gtgaacgcga aatatgccga
     1501 ataccgcgcg gcgcagtgca tccggcgctg gtgcgacccg gcgtacgagg tggtgccgcc
     1561 gttcgaggac tgggagaccg cgctgcatgc gccgccgtcg cgcagcgacg cgaagccctg
     1621 gccgcccggc gtgccccggc cgtgatgcgg gcggaaacat cccgacaccg tcgcccgcaa
     1681 cgggtcgccc gcccgcgtcg ctaaactgcg gcgcgccatg agctcgcccc acaccaccgc
     1741 cgccctgcat cgtctccggc cgctcgcgct cgccgccgcg ctggcggggg cactcgtcgg
     1801 cttcgggccc gcccgtgcca cggacgcgct ctcggccgcc atcccgtccg acctgcccga
     1861 cgcccgcctc gacttcggcg accgccacgt cggcctgccc gcggtcggcc gctggacgct
     1921 ggtgcagcgc gaggagcggc cgctcgcgga gcgccgcgac aagggctcgg tccacgagac
     1981 ctgggccgtc gcgatggacg gcccgcgcgt gcgcatggtg gtccggctga tgatgccgag
     2041 cgccggcgcg catcgcacgc gccgcggcgt cctgtcgccg tgcctggagc cggaaggcat
     2101 cctgcgcgag gatctctcga gcagggccgg cctgcccgag tgcctcgcga tcttcggcca
     2161 cgagaacctg cgcggcatct tccgccgccg ggcgcagccg accgcgagct ggatcgcggc
     2221 gcatgccgag gtcgatccct ccgcgccggc ggtcgagttc gcgtacacca agcgcgccga
     2281 cacggcattc ggctcggtgc gcatcttcac gaccaccgac gcgttccccg ccgacgacga
     2341 cgc
//
LOCUS       DASVLN010000213         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_386057, whole genome shotgun sequence.
ACCESSION   DASVLN010000213 DASVLN010000000
VERSION     DASVLN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386057"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VF453_04840"
     CDS             complement(<1..540)
                     /locus_tag="VF453_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX7687011.1"
                     /translation="MLSGFTPPYTPSGRSALIPPPPWHYAGQLMSLAFRLDAARAQEL
                     LPEGLGTATGAGAAHFCEWQSTSDGSELLDPAYAQYREFIALVEVERAGGRCFYCPFI
                     YVDQDLSMARGWLQGWPKKSGSVWMTRSYDLDHPAAAPARAGTRFGASLAVKDRRLAE
                     ALVTLSGEPAQPIGLLAAPA"
     gene            675..1151
                     /locus_tag="VF453_04845"
     CDS             675..1151
                     /locus_tag="VF453_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7687012.1"
                     /translation="MPRTLDNHDSRILRALQDDGRLTVAELAEKVHLSTSPTWRRVKR
                     LEDEGVIVGYQAMLDRRALGWGVLAFVMVTIGDHGDAEARAFEQAVGDLPEIVACWSI
                     AGSSDFLLQVVGQDLDTYADFAMTIVRRLPGIKAMQTILTLKEVKAPMPWPVPRAA"
     gene            complement(1148..2434)
                     /locus_tag="VF453_04850"
     CDS             complement(1148..2434)
                     /locus_tag="VF453_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7687013.1"
                     /translation="MFATPVARWLGRRGVHFSWIVAALAFLTMLSSSAALGLPGAFLR
                     PISAEFGWTTEQVSSALALRFMLYGLIGPFAAVLMERYGLRRIVCGALSLIALGLALA
                     TRMTALWQLVVLWGVVLGVGSGMTALVLGATVANRWFEQRKGLVLGLLTASSATGQLV
                     FLPIAAALIERHGWRVAVLPSFAMCLVVAGLAFCLLRDHPDELGLHPFGADPVRADAP
                     ARRWKPGFAEPVRALAAASRSLTFWVLFGTFFVCGLSTNGLIQTHFISLCGDYGLSPV
                     PAASVLATIGAFDFVGTIASGWLSDRFDNRKLLFWYYGLRGLSLLWLPHSGFTLIGLS
                     LFAVFYGLDWVATVPPTVKLSAATFDKQRAAMIFGWIFAGHQLGGAVAAYGAGLARTV
                     MASYSPALYAAGAACLLAAVGIFLVRKPAATAAVRV"
BASE COUNT          420 a          876 c          936 g          294 t
ORIGIN      
        1 cgccggcgcg gccagcaggc cgatgggctg cgccggctcg ccgctgagcg tgaccagcgc
       61 ctcggcgagc cgccgatcct tcaccgcgag gctcgcgccg aagcgggtgc cggcgcgcgc
      121 gggcgctgcg gccgggtgat cgaggtcgta gctgcgcgtc atccagacgc tgcccgactt
      181 cttgggccag ccctgcagcc agccgcgcgc catcgacagg tcctggtcga cgtagatgaa
      241 cggacagtag aagcagcgcc cgccggcgcg ctccacctcg acgagcgcga tgaactcgcg
      301 gtactgcgcg taggccgggt cgagcagctc gctgccgtcg gaggtggact gccattcgca
      361 gaagtgcgcg gcgccagcgc cggtggcggt gcccaggccc tctggcagca gctcctgcgc
      421 cctggccgca tcgagccgga aggccaggga catcaactgg cccgcgtagt gccagggcgg
      481 gggcgggatc agcgccgagc ggcccgacgg ggtgtacgga ggcgtgaagc cggaaagcat
      541 ggtgcgggtt ccttcgttgt tcgatgaaga cccatgctat tgcggtcgac cgggaaatgc
      601 ctgcctttct ctgccaggat cggcgggatt cgaggcatgc gaactatgat tcctagcctc
      661 gaattggaat gctcatgcct cgcaccctcg acaaccacga ttcccgcatc ctgcgcgcgc
      721 tccaggacga cggccgcctg acggtcgccg agctcgccga gaaggtgcac ctgtcgacct
      781 cgccgacctg gcgccgcgtc aagcggctgg aggacgaggg cgtgatcgtc ggctaccagg
      841 cgatgctgga tcgccgcgcg ctcggctggg gcgtgctggc attcgtgatg gtgacgatcg
      901 gcgaccacgg cgacgcggag gcgcgcgcgt tcgagcaggc cgtcggcgac ctgcccgaga
      961 tcgtcgcgtg ctggagcatc gccggcagct cggacttcct gctgcaggtc gtcggccagg
     1021 atctcgatac ctacgccgac ttcgcgatga ccatcgtccg ccgcctgccc ggcatcaagg
     1081 cgatgcagac gatcctgacg ctcaaggagg tcaaggcgcc catgccctgg cccgtgccgc
     1141 gggccgccta gacccggacc gcggcggtgg cagccggctt gcgcacgagg aagatcccga
     1201 ccgccgccag caggcaggcg gcgcccgcgg cgtacagcgc cgggctgtac gaggccatga
     1261 cggtgcgtgc gaggcccgcg ccgtaggccg ccaccgcgcc gccgagctgg tggccggcga
     1321 agatccagcc gaagatcatc gcggcgcgct gcttgtcgaa cgtggcggcc gaaagcttga
     1381 ccgtcggcgg caccgtggcg acccagtcca ggccgtagaa gacggcgaac agcgacagcc
     1441 cgattagcgt gaagcccgaa tgcggcagcc acagcagcga cagcccgcgc agcccgtagt
     1501 accagaacag cagcttgcgg ttgtcgaagc ggtcggacag ccagcccgag gcgatcgtgc
     1561 cgacgaagtc gaacgcgccg atcgtcgcca gcaccgaggc cgcgggcacg ggcgacaggc
     1621 cgtagtcgcc gcacagcgag atgaagtggg tctggatcag gccgttggtc gacagcccgc
     1681 agacgaagaa cgtgccgaac agcacccaga acgtgaggct gcgagaggcc gccgccaggg
     1741 cccgcacggg ctccgcgaag ccgggcttcc agcgccgggc cggggcgtcg gcgcgcacgg
     1801 gatcggcgcc gaacggatgc aggccgagct cgtccggatg gtcgcgcagc aggcagaacg
     1861 ccaggcccgc gacgacgagg cacatcgcga acgacggcag caccgccacg cgccagccgt
     1921 ggcgctcgat cagggcggcg gcgatcggca ggaacacgag ctggccggtc gcggagctcg
     1981 cggtcagcag gcccagcacc aggcccttgc gctgctcgaa ccaccggttg gcgacggtcg
     2041 cgcccagcac cagcgcggtc atgcccgagc cgacgcccag cacgacgccc cacagcacga
     2101 cgagctgcca cagcgccgtc atgcgcgtgg ccagcgcgag gcccagcgcg atcagcgaca
     2161 gcgcgccgca cacgatgcgc cgcaggccgt agcgctccat cagcaccgcg gcgaacgggc
     2221 cgatgaggcc gtagagcatg aagcgcagcg cgagcgccga cgacacctgc tcggtcgtcc
     2281 agccgaactc ggcggagatc ggccgcagga acgcgccggg caggcccagc gcggccgagg
     2341 agctgagcat ggtcaggaag gcgagggcgg cgacgatcca cgagaagtgg acgccgcggc
     2401 ggcccagcca tcgggcgacg ggcgtggcga acacggctca ggcctccttg cgggcggggc
     2461 ggcgcgcgcg ttggcgtgcc ggggcggcgg gcacggtcgc gggcgatggt tcgggcagca
     2521 gccgct
//
LOCUS       DASVLN010000214         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_279689, whole genome shotgun sequence.
ACCESSION   DASVLN010000214 DASVLN010000000
VERSION     DASVLN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279689"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1597
                     /gene="argA"
                     /locus_tag="VF453_04855"
     CDS             251..1597
                     /gene="argA"
                     /locus_tag="VF453_04855"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEX7687014.1"
                     /translation="MSLDLVFPHTFVPWFRAVAPHIHAYHGKTFVVAITGEAIAAHKL
                     NALAQDLAILHAMGMKLVLVHGFRPQVNDQLKAKNHPARYSHGIRITDAFALDCAQEA
                     AGQLRFEIEAAFSQGLPNTPMANATVRVVSGNFLTARPVGIVDGVDFMHSGVVRKVDG
                     AAIRSALDIGAVVLLSPFGFSPTGEAFNLTMEEVATSTAVALQADKLLFLTEVPGIHE
                     VQGDPESPIDTELALADAERLVATLPAPTVPTDTTFYLQHCVKACQGGVERSHILPFS
                     VDGALLMEIFTHDGIGTMVVDEKLESLREATSDDVGGILHLIEPFERDGTLVKRSRTE
                     IERDVGTYTVIEHDGIIFGCAALYPYPEAGTAELAALTVSPQVQSQGDGERILKRIEQ
                     RAKALGLKSIFVLTTRTMHWFIKRGFEQVDADWLPEARKKKYNWDRRSQVLVKKLP"
     gene            1614..1886
                     /locus_tag="VF453_04860"
     CDS             1614..1886
                     /locus_tag="VF453_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEX7687015.1"
                     /translation="MPRTVQCVYLKREAEGLDFPVYPGELGKRIYDNVSKEAFEAWKR
                     HQTMLVNENRLNLADARARQYLARQMEQFFFGGGAEQPAGYVPPTA"
     gene            2083..2388
                     /locus_tag="VF453_04865"
     CDS             2083..2388
                     /locus_tag="VF453_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEX7687016.1"
                     /translation="MSSLQELLAQKAEIDRQISDARRQERNEAISKVRALMAEHGLTA
                     ADLAAKAPTARASTSGRKVAAKYRDPVSGQTWTGRGLKPKWLAAALESGKQLADFAV"
     gene            2505..>3839
                     /gene="dnaG"
                     /locus_tag="VF453_04870"
     CDS             2505..>3839
                     /gene="dnaG"
                     /locus_tag="VF453_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7687017.1"
                     /translation="MIPGSFLQDLLARIDVVELVGRTVQLKKAGINYKGLCPFHGEKS
                     PSFIVSPTRQTYHCFGCGVHGDAIRFLVEQHGMGFVDAVKELAQQVGMAVPEDDATPE
                     ERAETARKREQSASLTDVLARACEHYKAQLRASPRAIDYLKGRGLSGEIAARFALGYA
                     PDGWRTLASAFPRYDDPLLVESGLVIVSGDDPATQKRYDRFRDRVMFPIRNVKGEIIG
                     FGGRVLDKGEPKYLNSPETPVFVKGRELYGLFEARQGLRERGYVLVTEGYMDVVALAQ
                     LGFPNAVATLGTACTAEHVHKLLRFTDKVVFSFDGDAAGRRAAGRALEAALPHANDTR
                     SFRFLFLPPEHDPDSYIREHGAAAFEQYVQKALPLSQQLMEMAAQDCDMATVEGRARM
                     LAQARPLWSALPDGALRRQMLAELAAKGHLPTDELDRLWQNGRQGGRGAGRAG"
BASE COUNT          613 a         1377 c         1292 g          557 t
ORIGIN      
        1 ggcgggcgtg ccgggtctcg tggcgagcgc tgcacgcggc gttcgggcgc ggcgatctcg
       61 gcggtcgggg cgtccgccgg ggcggcaggc gccgtggcgg cgggacgggg aggacgggtg
      121 ggcgaattca cgagcccgcg attatcgcaa gaccgacgcg cgcgccgggc gcggccctcg
      181 cggggtcatg gggcggtgtt gcacaatgcc gggctgacgt ttgacgacaa ggccgcccac
      241 cgcgcccgac atgagcctcg acctggtctt cccccacacc ttcgtgccgt ggttccgcgc
      301 cgtggcaccg cacatccacg cctaccacgg caagaccttc gtcgtggcga tcacgggcga
      361 ggcgatcgcc gcgcacaagc tcaacgccct cgcgcaggac ctcgccatcc tgcatgcgat
      421 gggcatgaag ctggtgctcg tgcacggctt ccggccgcag gtcaacgacc agctcaaggc
      481 caagaaccac ccggcgcgct acagccacgg catccgcatc accgacgcgt tcgcgctcga
      541 ctgcgcgcag gaggccgcgg gccagctgcg cttcgagatc gaggcggcgt tctcgcaggg
      601 cctgcccaac acgccgatgg ccaacgcgac cgtgcgcgtg gtgtcgggca acttcctgac
      661 cgcccggccg gtgggcatcg tcgacggcgt cgatttcatg cacagcggcg tcgtgcgcaa
      721 ggtcgacggc gcggcgatcc gcagcgcgct ggacatcggc gcggtcgtcc tgctgtcgcc
      781 gttcggcttc tcgcccaccg gcgaggcctt caacctgacg atggaggagg tcgcgacctc
      841 caccgccgtg gcgctgcagg ccgacaagct gctcttcctg accgaggtgc cgggcatcca
      901 cgaagtgcag ggcgaccccg aatcgccgat cgacaccgag ctcgccctgg ccgacgccga
      961 gcgcctggtc gccaccctgc cggcgcctac ggtgccgacc gacaccacgt tctacctgca
     1021 gcattgcgtc aaggcctgcc agggcggcgt cgagcgctcg cacatcctgc cgttctcggt
     1081 ggacggcgcg ctgctgatgg agatcttcac gcacgacggc atcggcacga tggtcgtcga
     1141 cgagaagctc gagagcctgc gcgaggcgac gtccgacgac gtgggcggca tcctgcacct
     1201 gatcgagccg ttcgagcgcg acggcacgct ggtcaagagg agccgcacgg aaatcgagcg
     1261 cgacgtcggc acttataccg tcatcgagca cgacggaatc attttcggct gcgccgcgct
     1321 gtatccctac cccgaggccg gcaccgcgga attggcggcg ctcacggtat ccccccaggt
     1381 gcagagccag ggcgacggcg agcgtatcct caagcgaatc gagcagcgcg ccaaggcgct
     1441 cggcctgaag tcgatattcg tgctgaccac gcgcacgatg cattggttca tcaagcgcgg
     1501 cttcgagcag gtcgatgccg attggctgcc cgaggcgcgc aagaagaaat acaactggga
     1561 ccggcgctcg caagtactgg tcaagaaact gccctgaaca ctggagctcc gtcatgcccc
     1621 gcaccgtcca atgcgtctac ctcaagcgcg aggccgaagg tctcgacttc cccgtctacc
     1681 cgggcgagct cggcaagcgc atctacgaca acgtcagcaa ggaagccttc gaggcgtgga
     1741 agcgccacca gacgatgctc gtcaacgaga atcggctgaa cctcgccgac gcgcgcgccc
     1801 gccagtatct ggcgcgccag atggaacaat tcttcttcgg cggcggcgcg gagcagcccg
     1861 ccggatacgt cccgcccacg gcctgaacga cgacccgcga taccggtcgc gtacgcgttt
     1921 ctcctgctcg gccccgagaa atattcctgc gccatcgtgt atattgattc ggcgtggccg
     1981 cgcctcgtgc gaagtgtcac gctccttccc gtgcgcgagt ttctggtcgc gaatttcgcg
     2041 ataagatgtt tcagttgccg aattgacacg aggatttgag acatgtcttc cttgcaggaa
     2101 ctgctggccc agaaggccga aatcgaccgc cagatttccg atgcccgccg ccaggaacgc
     2161 aacgaagcga tctccaaggt tcgcgcgctg atggccgagc acggcctgac cgccgccgat
     2221 ctcgccgcca aggcgcccac cgcccgggcg agcaccagcg gccgcaaggt cgccgccaag
     2281 taccgcgatc cggtcagcgg ccagacctgg accggacgcg gcctgaagcc gaagtggctc
     2341 gccgccgcgc tggagtcggg caagcagctg gccgacttcg cggtctgacg cgcgcgggcc
     2401 cgggcgcccg ccggccgcct cgccctttcc tgtcgagcgc ctgcgggcgc tcgatgcgtt
     2461 tgcggcgcgc gtcgcgccac gccgccctgc cctacactgc cccggtgatt cctggctcct
     2521 tcctgcaaga cctgctcgcg cgcatcgacg tcgtcgagct ggtcggccgc accgtccagc
     2581 tgaagaaggc cggcatcaac tacaagggcc tgtgcccgtt ccacggcgag aagtcgccca
     2641 gcttcatcgt cagcccgacg cgccagacct accactgctt cggctgcggg gtccacggcg
     2701 acgcgatccg cttcctggtc gagcagcacg gcatgggctt cgtcgacgcg gtcaaggagc
     2761 tggcgcagca ggtcggcatg gccgttcccg aggacgacgc cacgcccgag gagcgcgccg
     2821 aaaccgcccg caagcgtgag cagagcgcct cgctgaccga cgtgctcgcg cgcgcctgcg
     2881 agcattacaa ggcgcaactg cgcgcctcgc cgcgggcgat cgactacctc aagggccgcg
     2941 gcctgagcgg cgagatcgcg gcacgcttcg cgctcggcta cgcgcctgac ggctggcgca
     3001 cgctggcgag cgccttcccg cgctacgacg acccgctgct ggtggagtcg gggctcgtga
     3061 tcgtctccgg agacgacccg gcgacgcaga agcgctacga ccgcttccgc gaccgcgtca
     3121 tgttcccgat ccgcaacgtc aagggcgaga tcatcggctt cggcggccgg gtgctggaca
     3181 agggcgagcc gaagtacctg aactcgccgg agacgccggt cttcgtcaag ggtcgcgagc
     3241 tctacggcct gttcgaggcc cgccaggggc tgcgcgagcg cggctacgtg ctcgtcaccg
     3301 agggctacat ggacgtcgtg gcgctggcgc agctgggctt tcccaacgcc gtcgcgacgc
     3361 tcgggacggc ctgcacggcc gagcacgtgc acaagctgct gcgcttcacg gacaaggtgg
     3421 tcttcagctt cgacggcgac gccgccgggc gccgcgccgc gggccgcgcg ctcgaggccg
     3481 ccctgcccca cgcgaacgac acgcgcagct tccgcttcct cttcctgccg cccgagcatg
     3541 acccggacag ctacatccgc gagcacggcg ccgccgcctt cgagcagtat gtccagaagg
     3601 cgctgccgct gtcccagcag ctcatggaga tggccgccca ggattgcgac atggccaccg
     3661 tcgagggccg cgcccgcatg ctcgcgcagg cccggccgct gtggagcgcc ctgcccgacg
     3721 gcgcgctgcg gcgccagatg ctggccgagc tggccgccaa gggccacctg cccacggacg
     3781 agctcgaccg gctgtggcag aacggacgcc agggtgggcg cggggccggc cgggcgggc
//
LOCUS       DASVLN010000215         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_333276, whole genome shotgun sequence.
ACCESSION   DASVLN010000215 DASVLN010000000
VERSION     DASVLN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333276"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VF453_04875"
     CDS             <1..966
                     /locus_tag="VF453_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687018.1"
                     /translation="SVLVGVAHRVDAEWAIVDLRSGATRLLPRSRANPHQGGWDLWWA
                     SAPVAQTLLRVNSLGDVEFLDRDSLRPVGGFSLASLPGTRRPQFFGDVKLSPDGRYVL
                     TYWKRDIRQEDPELVIFDRQGRIVESGSPLDYEPLPLLGAFDWLPDGRYLYLAGKRIA
                     VRQIGNPKYLVAPLRLPPDVDVNHASVAVSPDGRHLALALAHDIVNGQRVRVGYNLLF
                     LSALDGTGLHQVTQPSAIMLDHGISMGHTDPVWSPDGRWVAFTPRQGGAYGAAHYGNP
                     CVDVLALPVDTGLATIDGLRDPPSLQLQAGGAPVVSCSTVQWVTP"
     gene            1142..1795
                     /gene="sctR"
                     /locus_tag="VF453_04880"
     CDS             1142..1795
                     /gene="sctR"
                     /locus_tag="VF453_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HEX7687019.1"
                     /translation="MDLTQFSPSSALVTVVLLALAPFVAVMVTSFTKIVVVLSLLRNA
                     LGLQQSPPNVVLNGLALVLSIYIMFPVMQQMSDRVQGYDNIAGNTKTMLAAADTAKEP
                     LRDFLVRHAPPRERAFFLKTAQKALTQDRAAQLTDRDFLVVVPAFTVSELSAAFQIGF
                     LIFLPFLVIDLVISNILMAMGMMMLSPTTVSMPFKLLLFVLVDGWVKLTHGLVLTYH"
     gene            1807..2076
                     /gene="sctS"
                     /locus_tag="VF453_04885"
     CDS             1807..2076
                     /gene="sctS"
                     /locus_tag="VF453_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctS"
                     /protein_id="HEX7687020.1"
                     /translation="MNNDALQLTQQALWFVMMLSAPPIAVAALVGIVVAVLQAATQIQ
                     EQTLQYALKFFAIVVTIFLTASMLGGGLYQFADRVFTGFAGMVVH"
     gene            2092..2886
                     /gene="sctT"
                     /locus_tag="VF453_04890"
     CDS             2092..2886
                     /gene="sctT"
                     /locus_tag="VF453_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HEX7687021.1"
                     /translation="MDALANVGLWSSQVLLIGLSTTRIAVAFLLLPLFTPDTMPVLVR
                     NAVFLALGGLSVALQPAVDVRHLDALDWLRLFGKEVFLGGAIGLLFGSVLWAFDVAGQ
                     IIDLKTGASMAQVVDPLSGHQTPLSAALLTRVASVVFMSSGGFLLLVGLMLQSYVLWP
                     VNATLPALRPGGFTLLEAEFGRLATLGLVISGPFLVVLYLVEAVFGLLNRYAQQLNVF
                     SLSASVKVLAAQALLILLLSTFGQLLVDDISARPPIVLRSLKAMLR"
     gene            2968..3183
                     /locus_tag="VF453_04895"
     CDS             2968..3183
                     /locus_tag="VF453_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687022.1"
                     /translation="MDITSSSLAHLGLSSGWSAPSTVGAVDEGAHDPASWFRADASAA
                     RIALPAATPEAADVASLARRVLAHLAA"
BASE COUNT          409 a         1184 c         1097 g          553 t
ORIGIN      
        1 agcgtgctgg tgggcgtcgc gcatcgcgtc gacgccgaat gggccatcgt cgacctgcgc
       61 agcggcgcga cgcggctgct cccgcgcagc cgtgcgaacc cgcaccaggg cggctgggac
      121 ctgtggtggg cgagcgcgcc ggtggcgcag acgctgctgc gcgtcaacag cctgggcgac
      181 gtcgagttcc tcgaccgcga ctcgctgcgg ccggtcggcg gcttctcgct cgcctcccta
      241 cccggcaccc ggcgacccca gttcttcggc gacgtcaagc tctcgcccga cggccgctac
      301 gtgctgacgt actggaagcg cgacatccgc caggaggatc cggagctggt gatcttcgac
      361 cgccagggac gcatcgtcga gagcggctcg ccgctggact acgagccgct gccgctgctc
      421 ggcgccttcg actggctgcc cgacggccgc tacctctacc tcgccggcaa gcgcatcgcg
      481 gtgcgccaga tcggcaatcc gaagtacctc gtcgcgccgc tgcgcctgcc gcccgacgtc
      541 gacgtcaacc acgcgagcgt cgccgtcagc cccgacggcc gccacctcgc gctggcgctc
      601 gcgcacgaca tcgtcaatgg ccagcgggtg cgcgtcggct acaacctcct gttcctgtcg
      661 gccctggacg gcaccggcct gcaccaggtg acgcagcctt cggccatcat gctggatcac
      721 ggcatcagca tgggccacac cgatccggtc tggtcgcccg acggccgctg ggtcgcgttc
      781 acgccgcgcc agggcggggc ctacggcgcc gcgcattacg gcaatccctg cgtggacgtg
      841 ctcgcgctgc ccgtcgacac cggcctggcc accatcgacg gcctgcgcga cccgccgtcc
      901 ctgcaactgc aggccggcgg cgcgcccgtg gtctcgtgca gcacggtgca gtgggtcacg
      961 ccctgagcaa ccggcgcctc gcatagggcg gcgggtatcg gcggcgcgcg gcggcgcccc
     1021 tgggaacgct cccaggctgg gaacgctccc agctggtggc cctcccggcc atcggacaca
     1081 ctgcggcccg accccaacgt tcagcatgag gagagggccc ccagggcacg ggccccgatc
     1141 gatggacctg acgcagttct cgccgtcctc ggcgctggtg accgtcgtgt tgctggcgct
     1201 ggcgccgttc gtggcggtga tggtcacctc cttcaccaag atcgtcgtgg tgctgagcct
     1261 gctgcgcaac gccctggggc tgcagcagtc gccgccgaac gtggtgctca acggcctcgc
     1321 gctggtgctc agcatctaca tcatgttccc ggtgatgcag cagatgtccg accgcgtcca
     1381 gggctacgac aacatcgccg gcaacacgaa gaccatgctc gccgcggccg acaccgccaa
     1441 ggagccgctg cgcgacttcc tcgtacgcca tgcgccgccg cgcgagcgcg cgttcttcct
     1501 gaagaccgcg cagaaggcgc tgacccagga tcgcgcggcg cagctgacgg atcgcgactt
     1561 cctcgtcgtc gtgccggcct tcaccgtcag cgagctctcg gccgcgttcc agatcggctt
     1621 cctgatcttc ctgcccttcc tcgtcatcga cctcgtcatc tccaacatcc tgatggcgat
     1681 ggggatgatg atgctgtcgc cgaccaccgt ctcgatgccg ttcaagctgc tgctgttcgt
     1741 gctcgtggac ggctgggtga agctcacgca cggcctcgtg ctcacctacc actgaggacg
     1801 ccccccgtga acaacgacgc gctgcagctg acccagcagg ccctctggtt cgtgatgatg
     1861 ctgtcggcgc cgccgatcgc ggtggcggcg ctggtgggca tcgtggtcgc ggtgctgcag
     1921 gcggcgacgc agatccagga gcagacgctg cagtacgcgc tgaagttctt cgcgatcgtc
     1981 gtcaccatct tcctgaccgc ctcgatgctg ggcggcgggc tctaccagtt cgcggaccgg
     2041 gtgttcaccg gcttcgccgg catggtcgtg cactaggccg gatcgccgcg gatggacgcg
     2101 ctcgccaacg tcggcctgtg gtcgtcgcag gtcctgctga tcgggttgtc gaccacgcgc
     2161 atcgcggtcg cgttcctgct cctgccgctc ttcaccccgg acacgatgcc cgtgctggtg
     2221 cgcaacgcgg tgttcctcgc gctgggcggc ctgtcggtgg cgctgcagcc ggccgtcgac
     2281 gtgcggcacc tcgacgcgct cgactggctg cgcctgttcg gcaaggaggt cttcctcggc
     2341 ggggccatcg gcctgctgtt cggctccgtg ctgtgggcct tcgacgtcgc cggccagatc
     2401 atcgacttga agaccggcgc ttcgatggcg caggtcgtcg acccgctgtc gggccaccag
     2461 acgccgctgt cggcggccct gctcacgcgc gtggcgagcg tcgtgttcat gtcctcgggc
     2521 ggcttcctgc tgctcgtcgg cctgatgctg caaagctatg tgctctggcc cgtgaacgcg
     2581 acgctgcccg cgctgcggcc cggcggattc acgctgctcg aagcggagtt cgggcgtctc
     2641 gcgacgctcg gcctcgtgat cagcgggccg ttcctcgtcg tgctgtatct cgtggaggcc
     2701 gtcttcgggc tgctgaaccg ctacgcgcag cagctcaacg tgttctcgct gtcggcgtcg
     2761 gtgaaggtgc tggccgcgca ggcgctgctg atcctgctgc tgtcgacgtt cgggcagctg
     2821 ctggtcgacg acatcagcgc gcgcccgccc atcgtgctgc gcagcctgaa ggcgatgctg
     2881 cgctgaaccg gggcccgccg ccctgggaac gatgccagtg ggcggcgcgc gctcgcttgg
     2941 ctagagtggc gtcaacgcag gttcatcatg gacatcacct cttcctcgct cgcgcatctc
     3001 ggcttgtcgt ccggctggtc ggcaccatcg accgtgggcg ccgtcgacga aggcgcgcac
     3061 gatccggcgt cgtggttccg cgccgacgcc agcgccgcgc gcatcgccct gcccgccgcg
     3121 acgccggagg ccgccgacgt cgcgagcctt gcgcgacgcg tgctggccca tctcgccgcc
     3181 tgaaggccgg ggaggcgcgg tgctcaagcc gcagcccgac tggctcgcgg cggcgcaggc
     3241 gct
//
LOCUS       DASVLN010000216         6112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_172424, whole genome shotgun sequence.
ACCESSION   DASVLN010000216 DASVLN010000000
VERSION     DASVLN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172424"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VF453_04900"
     CDS             <1..304
                     /locus_tag="VF453_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7687023.1"
                     /translation="HAAPPTVATTPTATPDTSGAQAAAGGRFVVQIGAFSDDAKARDA
                     RGKVEKLGLKTYTQAVETPSGKRVRVRVGPYATKAEADRAAARIKSDGLPAAVLSL"
     gene            345..887
                     /locus_tag="VF453_04905"
     CDS             345..887
                     /locus_tag="VF453_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX7687024.1"
                     /translation="MNDLQQLGWVDLTLLAILVVSVLIGLWRGLTFELVSLLGWLVAF
                     VIANSCGPLLADVVPLGEPGSGLRLWICYAIVFFAVLVSCTLLARMLRALIAATPLSV
                     VDRLLGGVFGVARGALILVILATLVSLSPVARSDTWRASHGALWLGQALEVMRPVLPQ
                     SLAAHLSSAAAAEDVEPVTA"
     gene            967..2484
                     /gene="purF"
                     /locus_tag="VF453_04910"
     CDS             967..2484
                     /gene="purF"
                     /locus_tag="VF453_04910"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX7687025.1"
                     /translation="MCGIVGVISKAPVNQLIYDSLLLLQHRGQDAAGIVTMQGHKCFM
                     HKARGMVRDVFRTRNMRALPGTVGLGQVRYPTAGNAYSEEEAQPFYVNAPFGIVLVHN
                     GNLTNAHALKAELIDKDRRHINTESDTEVLINILAHELERVARGSTLTPDVVFDAVAA
                     VHKRIKGSYAVVALIAGHGLLAFRDPFGIRPLIYGETETPEGRSVMVASESVALEGTG
                     HQVVRDVAPGEALFIDMDGGVHTRQCAENPTLHPCMFEYVYLARPDSRIDGISVYQAR
                     LNMGETLAQRLISTMPPSEIDVVIPIPESSRPSAMQLAQKIGKPYREGFVKNRYVGRT
                     FIMPGQSVRKKSVRQKLNAIGVEFKGRNVLLVDDSIVRGTTSKEIVQMAREAGANKVY
                     MASAAPPVKYPNVYGIDMPTPEELIASGRTMEEIREFIGADALIYQDVDAMKRVVGRL
                     NKDIVGFEASCFDGCYITGDVSSDDFKAIEAQRRSQQEEEDGPGRSRLALQNASE"
     gene            2551..3948
                     /gene="gltX"
                     /locus_tag="VF453_04915"
     CDS             2551..3948
                     /gene="gltX"
                     /locus_tag="VF453_04915"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7687026.1"
                     /translation="MNDTPVRTRIAPSPTGFLHLGTARTALYSWAFARHHGGEFVLRI
                     EDTDVARSTRESVDQIIASMDWLGLSYDEGPIYQMQRLERYDAVVQGMLAAGTAYHCY
                     CTPEDLERMKDDQESRGEKRRYDGTWRPLPGKTLPPIPAGVKPVVRFANPVDGEVTWD
                     DLVKGPITVANKEIDDLIIVRDDGVPTYNFAVVVDDWDMRISHVFRGDEHINNTPWQI
                     NMFRALGAPLPKFGHLPIILGDDGLKLSKRRGAVSVTEYEANGYLPEGMNNYLARLGW
                     SHGDDELFTIEQMVSWFDGSHLNRSPAQWDAAKLLWVNAHHMKQADDARLAALVVGQL
                     ARRGISAQADDAMAARCALLKDRCQTTVELADWIEMFHAAPTPAQADLDQHVTDAART
                     ALRTLRGKLETAAWDKAGIAAAMKETLAEHAIKMPQLAIPLRVVVCGRAQTPAIDALI
                     ALFQREVVLDRLRTV"
     gene            4025..4100
                     /locus_tag="VF453_04920"
     tRNA            4025..4100
                     /locus_tag="VF453_04920"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4058..4060,aa:Ala,seq:ggc)
     gene            4154..4229
                     /locus_tag="VF453_04925"
     tRNA            4154..4229
                     /locus_tag="VF453_04925"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4188..4190,aa:Glu,seq:ttc)
     gene            4292..4368
                     /locus_tag="VF453_04930"
     tRNA            4292..4368
                     /locus_tag="VF453_04930"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4326..4328,aa:Asp,seq:gtc)
     gene            4386..4461
                     /locus_tag="VF453_04935"
     tRNA            4386..4461
                     /locus_tag="VF453_04935"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4420..4422,aa:Glu,seq:ttc)
     gene            4523..4599
                     /locus_tag="VF453_04940"
     tRNA            4523..4599
                     /locus_tag="VF453_04940"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4557..4559,aa:Asp,seq:gtc)
     gene            4787..5431
                     /locus_tag="VF453_04945"
     CDS             4787..5431
                     /locus_tag="VF453_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687027.1"
                     /translation="MPAPAFRLAPHARRLALAAIAFASASLAGCATPGVHAPDFVEPA
                     ASAPTAKLRIVNVRPQAYYAAIALFDSAACLDRANLGMTGGASKDTERVGMLDAKPVQ
                     AGILERRVPAGELLVVGPRAVFPTVTAGELMHAFEPQAQDATRARQAGVCRVPSFTPK
                     AGEQYEIVVDLTPARCSVTPYRLSQGDGGEVKREEVAAQASRISTYEFDMKCFK"
     gene            complement(5471..6013)
                     /locus_tag="VF453_04950"
     CDS             complement(5471..6013)
                     /locus_tag="VF453_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7687028.1"
                     /translation="MTADTPAPAFVLRPASRTDAPLIAELHARSRASAYRGLLPDDYL
                     DRAMPVEAAAHWAQRLPELAAGEGQALVAEIDGRPVGFVCMSAPDAQRSVYIENLHVL
                     PGHKGGGIGTRLLEVAAAWARERGATRLHLLVFEGNRAAIGFYESRGWRCVERLDDEL
                     GGHALPALVCALPLDAGPTG"
BASE COUNT          952 a         2146 c         2107 g          907 t
ORIGIN      
        1 gcatgcggca ccgccgaccg tcgcgacgac gccgacggcc acgcccgaca cgtccggcgc
       61 gcaggctgcc gccggcgggc gcttcgtcgt gcagatcggc gcattctccg acgacgccaa
      121 ggcccgcgat gcccgcggca aggtcgagaa gctgggcctg aagacctaca cgcaggccgt
      181 ggagacgccc tcgggcaagc gcgtgcgcgt gcgcgtgggc ccgtacgcga ccaaggccga
      241 ggccgaccgc gccgccgccc gcatcaagtc cgacggcctg ccggccgcgg tgctgtcgct
      301 gtgagcctgg ccgacgccgc ccgccgcgcc tgccgggagt cccgatgaac gacctgcagc
      361 agctcggctg ggtcgacctc acgctgctcg cgatcctcgt ggtgtcggtc ttgatcggcc
      421 tgtggcgcgg cctcacgttc gagctggtgt cgctgctggg atggctggtg gcgttcgtga
      481 tcgccaactc gtgcgggccg ctgctggcgg acgtcgtgcc gctgggggag ccgggctcgg
      541 ggctgaggct gtggatctgc tacgcgatcg tcttcttcgc cgtgctcgtc tcgtgcacgc
      601 tgctcgcgcg catgctgcgg gcgctcatcg cggcgacgcc gctgtcggtg gtcgaccgcc
      661 tgctgggcgg cgtcttcggc gtcgcgcgcg gcgcgctcat cctcgtgatc ctggcgacgc
      721 tcgtgtccct gtcgccggtc gcgcgatcgg acacgtggcg cgcctcgcac ggcgcgttgt
      781 ggctcgggca ggcgctggag gtgatgcggc ccgtgctgcc gcagtcgctc gcggcgcacc
      841 tgtcgagcgc cgcggcggcc gaagacgtgg agccggtcac ggcctgaatt cgccgccggc
      901 cggcggcccg cggcgcgaac gcggaaaatc ggcaccagtc ccccggaatt tgcaaggaac
      961 ccaactatgt gcggcatcgt cggcgtgatc tccaaggctc ccgtcaacca gctgatctac
     1021 gactccctgc tgctgctgca gcaccgcggc caggatgccg ccggcatcgt gacgatgcag
     1081 ggccacaagt gcttcatgca caaggcgcgc ggcatggtgc gggacgtctt ccgcacccgc
     1141 aacatgcgcg cgctgccggg taccgtgggc ctgggccagg tgcgctaccc gacggccggc
     1201 aacgcctaca gcgaggagga ggcgcagccc ttctacgtca acgcgccgtt cggcatcgtg
     1261 ctcgtgcaca acggcaacct gacgaacgcg cacgcgctga aggcggagct gatcgacaag
     1321 gatcgccgcc acatcaacac cgagagcgac accgaggtgc tgatcaacat cctcgcccac
     1381 gagctcgagc gcgtcgcccg cggctccacg ctgacgcccg acgtcgtctt cgacgcggtg
     1441 gccgccgtgc acaagcggat caagggctcg tatgcggtgg tcgcgctgat cgccgggcac
     1501 ggcctgctcg cgttccgcga cccgttcggc atccgtccgc tgatctacgg cgagaccgag
     1561 acgcccgagg gccgcagcgt gatggtcgcc agcgagagcg tcgcgctcga gggcaccggc
     1621 caccaggtcg tgcgcgacgt cgcgccgggc gaggcgctgt tcatcgacat ggacggcggc
     1681 gtccacacgc gccagtgcgc cgagaacccg acgctgcatc cgtgcatgtt cgagtacgtc
     1741 tacctcgcgc gccccgactc gcgcatcgac ggcatctccg tctaccaggc gcgcctgaac
     1801 atgggcgaga cgctcgccca gcgcctgatc tcgacgatgc cgcccagcga gatcgacgtc
     1861 gtcatcccga ttcccgagtc gagccggccg tcggcgatgc agcttgcgca gaagatcggc
     1921 aagccctacc gcgaggggtt cgtgaagaac cgctacgtcg gtcgcacctt catcatgccg
     1981 gggcagtcgg tgcgcaagaa gtcggtgcgc cagaagctca acgcgatcgg cgtcgagttc
     2041 aagggccgca acgtgctgct ggtcgacgac tcgatcgtgc gcggcacgac gtccaaggag
     2101 atcgtgcaga tggcgcgcga ggccggtgcg aacaaggtct acatggcctc ggcggcgccg
     2161 ccggtgaaat accccaacgt gtacggcatc gacatgccca cgcccgagga gctgatcgcc
     2221 tccggccgca cgatggagga gatccgcgag ttcatcggcg ccgacgcgct gatctaccag
     2281 gacgtggacg cgatgaagcg cgtcgtgggc cggctgaaca aggacatcgt ggggttcgag
     2341 gcctcctgct tcgacggctg ctacatcacg ggcgacgtga gctccgacga cttcaaggcg
     2401 atcgaggcgc agcgccgctc gcagcaggag gaggaggacg gcccgggccg ctcgcgcctc
     2461 gcgctgcaga acgcgtccga atgaacgccg cggccgccgc cggcgcctga tcgactcacc
     2521 cggcccgcgc cgggccgcaa aagacatccg atgaacgata cccctgtccg tacccgcatc
     2581 gccccgtcgc cgacgggctt cctccatctc ggcaccgcgc gcacggcgct gtactcgtgg
     2641 gcgttcgcgc gccaccacgg cggcgagttc gtgctgcgca tcgaggacac cgacgtcgcc
     2701 cgctcgaccc gggagtcggt cgaccaaatc atcgcgtcca tggactggct gggcctgtcc
     2761 tacgacgagg gcccgatcta ccagatgcag cggctggagc gctacgacgc ggtggtgcag
     2821 ggcatgctcg cggccggcac ggcgtaccac tgctattgca cgcccgagga tctcgagcgg
     2881 atgaaggacg accaggagtc gcgcggcgag aagcggcgct acgacggcac gtggcgtccg
     2941 ctgcccggca agacgctgcc gcccatcccc gcgggcgtca agccggtggt gcgcttcgcg
     3001 aacccggtcg acggcgaggt gacctgggac gatctcgtca agggcccgat cacggtcgcc
     3061 aacaaggaga tcgacgacct gatcatcgtg cgcgacgacg gcgtgcccac gtacaacttc
     3121 gccgtcgtgg tggacgattg ggacatgcgc atcagccacg tcttccgcgg cgacgagcac
     3181 atcaacaaca cgccctggca gatcaacatg ttccgcgcgc tcggcgcgcc gctgcccaag
     3241 ttcggccacc tgcccatcat cctcggcgac gacggcctga agctgtccaa gcgccgcggc
     3301 gccgtgagcg tgaccgagta cgaggccaac ggctacctgc ccgagggcat gaacaactac
     3361 ctcgcgcgcc tgggctggag ccatggcgac gacgagctgt tcacgatcga gcagatggtg
     3421 tcctggttcg acggctcgca cctgaaccgc agtcccgccc agtgggacgc ggccaagctg
     3481 ctgtgggtca acgcccacca catgaagcag gccgatgacg cgcgcctggc cgcactggtg
     3541 gtcgggcagc tcgccaggcg cggcatttcg gcgcaggccg acgacgccat ggcggcgcgc
     3601 tgtgcgctgc tgaaggaccg ctgccagacg accgtggagc tggcggactg gatcgagatg
     3661 ttccatgccg cgcccacgcc cgcgcaggcc gacctggacc agcacgtcac cgatgccgcg
     3721 cgcaccgcgc tgcgcacgct gcgcggcaag ctcgagaccg cggcctggga caaggccggc
     3781 atcgcggccg cgatgaagga gacgctggcc gagcacgcca tcaagatgcc gcagctcgcg
     3841 atcccgctgc gcgtggtcgt ctgcggccgc gcgcagacgc cggccatcga tgcgctgatc
     3901 gcgcttttcc aacgcgaagt tgtgctcgac cgcttgcgca ctgtttgaaa tctgttctat
     3961 acttgcggtc ttcgctcttc accacgcgat gcagcttcgg aagtcaagaa ccggggcgga
     4021 cattgggggt atagctcagc tgggagagcg cttgcatggc atgcaagagg tcagcggttc
     4081 gatcccgctt acctccacca tttgcccctc ggggcaggcc ggcagcgcgt ggcagggagt
     4141 ggaaacggat tttgacccta tcgtctagag gcctaggaca cgaccctttc acggtcggta
     4201 ccggggttcg aatccccgta gggtcgccag cgatagcggg cacaagttgc aggcaacgct
     4261 tggaacgcaa gttcgaagtt gccgaccacg tggagtggta gttcagttgg ttagaatacc
     4321 ggcctgtcac gccgggggtc gcgggttcga gtcccgtcca ctccgccacg aattgtttta
     4381 cgactggccc tatcgtctag aggcctagga cacgaccctt tcacggtcgg taccggggtt
     4441 cgaatccccg tggggtcgcc agtcaccaca cacaagccgc gtacggcgct tggagcgcga
     4501 gctcgaagcc ggacggccac gtggagtggt agttcagttg gttagaatac cggcctgtca
     4561 cgccgggggt cgcgggttcg agtcccgtcc actccgccaa gcatttgcaa agcagccacc
     4621 ttcgggtggc tgttttcatt tccgcgcaga tcgtccgaaa aaagtcgcgt cgaccacgcc
     4681 atcgtccggg cgtgccacat gtcgaggtcg tcgcgcgtgt gtcggggttg aaacgaacct
     4741 acaggtcgcg cgcaacatgg ccgatacacg agggcaggag acgcccatgc ccgcacccgc
     4801 ctttcgcctc gccccgcacg cccgccgact cgccctggcg gcgatcgcgt tcgcctcggc
     4861 ttcgctggcc ggctgcgcga cgccgggcgt ccacgcgccg gacttcgtcg agcccgcggc
     4921 cagtgcgccg actgccaagc tgcgcatcgt caacgtccgc ccgcaggcgt actacgccgc
     4981 gatcgcgctg ttcgattcgg ccgcctgcct cgaccgcgcg aacctcggca tgaccggcgg
     5041 cgcgagcaag gacaccgagc gcgtcggcat gctcgacgcc aagcctgtgc aggccggcat
     5101 cctcgagcgc cgcgtgcccg cgggcgagct gctggtcgtc ggcccgcgtg ccgtgttccc
     5161 gacggtcacc gcgggcgagc tgatgcacgc gttcgagccg caggcgcagg acgcgacgcg
     5221 ggcgcgccag gctggcgtct gccgcgtccc gagcttcacg cccaaggccg gcgagcagta
     5281 cgagatcgtc gtcgacctca cgcccgcgcg ctgcagcgtc acgccgtacc gcctgtcgca
     5341 gggcgacggc ggcgaggtca agcgcgagga ggtcgccgcg caggcgtcgc ggatctcgac
     5401 ctacgagttc gacatgaagt gcttcaagtg aggctcgcga cgcactgaag cgcccgcttg
     5461 cattgtccgt tcaaccggtg ggaccggcgt cgagcggcaa ggcgcagacc agcgccggca
     5521 gggcgtgccc gcccagctcg tcgtccaggc gctcgacgca gcgccagccg cgcgactcgt
     5581 agaagccgat cgcggcgcgg ttgccttcga acacgagcag gtgcaggcgc gtcgccccgc
     5641 gctcgcgcgc ccaggccgcc gcgacctcca gcaggcgcgt gccgatgcca ccgcccttgt
     5701 gtccgggcag cacgtgcagg ttctcgatgt agacgctgcg ctgcgcgtcg ggcgcgctca
     5761 tgcacacgaa gcccaccggc ctgccgtcga tctccgcgac cagcgcctgg ccctcgccgg
     5821 ccgccagctc gggcaggcgc tgcgcccagt gcgcggctgc ctccaccggc atcgcgcgat
     5881 cgaggtagtc gtccggcagc agcccgcggt aggccgaagc gcggctgcgc gcatgcagtt
     5941 cggcgatgag cggggcgtcg gtgcgcgagg cagggcgcag gacgaaggcg ggcgcggggg
     6001 tgtcggcggt catcgtggca gctcggatcg agcgcgcgtt ataccccggc gcgccgcttc
     6061 ggcgttcgcc ccgcgcggcg gcggcgggcc ctccgacgac ggcttcaaag ca
//
LOCUS       DASVLN010000217         3883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_441011, whole genome shotgun sequence.
ACCESSION   DASVLN010000217 DASVLN010000000
VERSION     DASVLN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441011"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1294
                     /gene="proB"
                     /locus_tag="VF453_04955"
     CDS             188..1294
                     /gene="proB"
                     /locus_tag="VF453_04955"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX7687029.1"
                     /translation="MRDARRIVVKVGSSLVTNEGRGVDAEAIGNWCRQMALLGRSGRE
                     IVMVSSGAIAEGMKRLGWTARPKELHELQAAAAVGQMGLAQMYESKLREHGFGSAQVL
                     LTHADLADRERYLNARSTLVTLLGLQVIPVINENDTVVTDEIKFGDNDTLGALVANLV
                     DADAYVILTDQRGLYTADPRKDPAARFVDRARAGDPELERMAGGAGSAIGRGGMLTKI
                     LAAKRASRSGTSTVIAWGRETDVLLRLAQGEPIGTLLAAETAKVAARKRWMVDHLQLR
                     GAVVVDDGAAIKLRDEGKSLLPIGVHEVEGDFRRGDVIAIRALDGRVVARGLANYSSA
                     EARLIARKPSSQIEAALGYANEPELVHRDNLVLG"
     gene            complement(1320..1952)
                     /locus_tag="VF453_04960"
     CDS             complement(1320..1952)
                     /locus_tag="VF453_04960"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX7687030.1"
                     /translation="MLDREGFRPNVGIVLLNSRNQVFWGKRLRTHSWQFPQGGIKYGE
                     SPEQAMFRELHEEVGLLPDHVKIIARTRDWLRYEVPEHYIRKDARGHYRGQKQIWFLL
                     KLMGRDSDMNLRATDHPEFDAWRWNDYWVPLDVVIEFKRGVYQLALTELARFLPKTNY
                     HNRYLRGGPRGPREDRSLEPAGVPSDLPPDAAAGESAPDSRLPAAGPSVN"
     gene            2081..3823
                     /locus_tag="VF453_04965"
     CDS             2081..3823
                     /locus_tag="VF453_04965"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX7687031.1"
                     /translation="MKASQFFVSTLKEAPADAEVTSHKLMTRAGMIKRLGAGIYNYMP
                     MGLRVIRKVEAIVREEMNRAGAVEVTMPVVQPAELWEETGRFGKMGPELLRLKDRHDR
                     DFVMQPTSEEVVTDIARQELRSYKQLPRNFYQIQTKFRDERRPRFGIMRGREFTMKDA
                     YSFDRDKAGADRSYQAMRSAYRAIFDRFGLRYRAVAADSGAIGGDLSEEFQVIADTGE
                     DAIIYCPTSDYAANIEKAEAVALIASRGPALEPMAKVATPGKSTCEDVAALLGLPLAR
                     TVKSIVLATDERDEAGDVVRTTVWLLLLRGDHELNEVKAGKVAGLEGSRWATTAEIEA
                     HFGCKPGYLGPVGMKEPVKVVADRTVANMSDWICGANEVDFHLAGVNWGRDLPEPDVV
                     ADIRNVVAGDPSPDGQGVLAIDRGIEVGHVFYLGTKYSKPMNATFLDENGKPQLFEMG
                     CYGIGVTRVVAAAIEQNHDARGIIWPVALAPFTVVLCPIGYDRSADVKAAADKLHDEL
                     AALGIDVLLDDRGERPGAMFADWELIGVPQRVVISDRGLKDGKLEVQGRREEAASAIA
                     LDDVVVEIQARLAQ"
BASE COUNT          604 a         1361 c         1378 g          540 t
ORIGIN      
        1 gagccggagg ccgagctgga ggcggtcgcc gcgccggccc cggcgcgccg cacgcgtcgc
       61 gcggcatgag gcgccgaacg gccgctccca agggggctcg gacggcgatc cgcaggatgg
      121 agctcgcctc gtgagcgccg gcaccgagcg cgtcgcgggc ggggtgccgc ctgcccccaa
      181 cctcgagctg cgcgacgcgc gccgcatcgt cgtcaaggtc ggctccagcc tcgtcacgaa
      241 cgagggccgc ggcgtcgatg cggaggcgat cggcaactgg tgccgccaga tggcactgct
      301 cggccggtcg ggtcgcgaga tcgtcatggt gtcctcgggc gcgatcgccg aaggcatgaa
      361 gcgcctgggc tggacggcgc ggccgaagga gctgcacgag ctgcaggccg cggccgccgt
      421 cggccagatg ggcctcgcgc agatgtacga gagcaagctg cgcgagcacg gcttcggcag
      481 cgcgcaggtg ctgctcacgc atgccgacct cgcggaccgc gagcgctacc tgaacgcacg
      541 ctccaccctc gtgacgctgc tcgggctgca ggtgatcccc gtcatcaacg agaacgacac
      601 ggtcgtcacc gacgagatca agttcggcga caacgacacg ctgggcgcgc tcgtggccaa
      661 cctggtcgat gccgacgcct atgtcatcct caccgaccag cgcggcctgt acaccgccga
      721 cccgcgcaag gatcccgccg cccgcttcgt cgaccgcgcc cgggccggcg accccgagct
      781 cgagcgcatg gccggcgggg ccggctcggc gatcgggcgc ggcggcatgc tgaccaagat
      841 cctcgccgcc aagcgcgcct cgcgctccgg cacgagcacc gtgatcgcgt ggggccggga
      901 gaccgacgtc ctcctgcgcc tcgcccaggg cgagccgatc ggcacgctgc tcgccgccga
      961 gaccgcgaag gtcgccgcgc gcaagcgctg gatggtcgac cacctgcagc tgcgcggcgc
     1021 ggtcgtggtg gacgacggcg ccgcgatcaa gctgcgcgac gaaggcaaga gcctgctgcc
     1081 gatcggcgtc cacgaggtcg agggcgactt ccgccgcggc gacgtgatcg cgatccgcgc
     1141 gctcgacggg cgcgtcgtgg cgcgcgggct ggcgaactac tcgtcggcgg aggcgcggct
     1201 gatcgcgcgc aagccgtcct cgcagatcga ggcggcgctg ggctatgcga acgagcccga
     1261 gctcgtccac cgcgacaacc tcgtgctggg ctgatcccgg cgtgggccgg gccgcgcgcc
     1321 tagttcaccg aagggccggc ggcgggcagg cggctgtccg gcgccgactc gccggccgcg
     1381 gcgtcgggcg gcaggtcgct cggcacgccc gccggctcca ggctgcggtc ctcgcgcggg
     1441 ccgcgcggtc caccgcgcag gtagcggttg tggtagttgg tcttgggcag gaagcgcgcg
     1501 agctcggtca gcgcgagctg gtagacgccg cgcttgaact cgatgacgac gtccagcggc
     1561 acccagtagt cgttccagcg ccaggcatcg aattccgggt ggtcggtcgc gcgcaggttc
     1621 atgtcgctgt cgcgacccat cagcttcagc aggaaccaga tctgcttctg gcccctgtaa
     1681 tggccgcgcg cgtccttgcg gatgtagtgc tcgggcacct cgtagcgcag ccagtcgcgc
     1741 gtgcgggcga tgatcttcac gtggtcgggc agcaggccga cttcctcgtg aagttcacgg
     1801 aacatcgcct gctcgggcga ctcgccgtat ttgatgcccc cttgcgggaa ctgccaggag
     1861 tgcgtgcgga gccgctttcc ccagaacacc tggttgcgcg agttgagcag gacaatgccg
     1921 acgttgggcc tgaagccctc tcggtcgagc ataatctgac cccgattctt gaactgaggt
     1981 cattattgca tcgggctcgc cgaaaacacg agcccgcgtt tttccgcata cgcggcagca
     2041 agcacgacac cttgatgaca aaccccggta acgtccgctc atgaaagcct cgcagttctt
     2101 cgtctccacg ctcaaggaag cccccgccga cgccgaggtc acgagccaca agctcatgac
     2161 ccgcgcgggc atgatcaagc gcctgggcgc aggcatctac aactacatgc cgatgggcct
     2221 gcgcgtgatc cgcaaggtgg aggccatcgt gcgcgaggag atgaaccgcg ccggtgccgt
     2281 cgaggtcacg atgccggtgg tccagccggc cgagctgtgg gaagagacgg gccgcttcgg
     2341 caagatgggc ccggagctgc tgcgcctgaa ggatcgccac gatcgcgact tcgtgatgca
     2401 gccgacctcc gaggaagtgg tcactgacat cgcgcgccag gagctgcgca gctacaagca
     2461 gttgccgcgc aatttctacc agatccagac caagttccgc gacgagcgcc ggccccgctt
     2521 cggcatcatg cgcggccgcg agttcacgat gaaggacgcg tactccttcg accgcgacaa
     2581 ggccggcgcc gaccggagct accaggcgat gcgcagcgcc taccgcgcga tcttcgaccg
     2641 cttcggcctg cgctaccgcg cggtggccgc ggactcgggt gcgatcggcg gcgacctgtc
     2701 cgaggagttc caggtcatcg ccgacaccgg cgaggacgcg atcatctact gcccgacctc
     2761 cgactacgcc gccaacatcg agaaggcgga ggccgtcgcg ctcatcgcct cgcgcgggcc
     2821 ggcgctcgag ccgatggcca aggtcgcgac gcccggcaag agcacctgcg aggacgtggc
     2881 ggcgctgctc gggctgccgc tcgcgcgcac ggtgaagtcc atcgtgctcg cgaccgacga
     2941 gcgcgacgag gccggcgacg tcgtcaggac gaccgtctgg ctgctgctgc tgcgcggcga
     3001 ccacgagctc aacgaagtga aggccggcaa ggtcgcgggc ctcgagggct cgcgctgggc
     3061 cacgacggcc gagatcgagg cgcatttcgg ctgcaagccg ggctacctcg gcccggtcgg
     3121 catgaaagag ccggtcaagg tcgtcgccga ccgcaccgtc gccaacatgt ccgactggat
     3181 ctgcggcgcc aacgaggtcg acttccacct cgccggcgtc aactggggcc gcgacctgcc
     3241 cgagccggac gtcgtcgccg acatccgcaa cgtcgtcgcg ggcgacccgt cgccggacgg
     3301 ccagggcgtg ctggcgatcg accgcggcat cgaggtcggc cacgtgttct acctcggcac
     3361 caagtattcc aagccgatga acgcgacctt cctcgacgag aacggcaagc cccagctgtt
     3421 cgagatgggc tgctacggca tcggcgtgac gcgcgtcgtc gcggcggcga tcgagcagaa
     3481 ccacgacgcg cgcggcatca tctggccggt cgcgctcgcg ccattcacgg tggtgctgtg
     3541 cccgatcggc tacgaccgca gcgccgacgt caaggccgcg gccgacaagc tccacgacga
     3601 gctcgcggcg ctgggcatcg acgtgctgct ggacgaccgc ggcgagcgcc ccggcgcgat
     3661 gttcgcggac tgggagctga tcggcgtgcc gcagcgcgtc gtgatctcgg atcgcggcct
     3721 caaggacggc aagctcgagg tgcagggccg ccgcgaggag gctgcctcgg ccatcgcgct
     3781 ggacgacgtc gtggtcgaga tccaggcgcg cctggcgcaa tgacggcagg catgcgccgg
     3841 cgcgcggtcg tcgtcggcct cgccgccggc gcgctggcgc tcg
//
LOCUS       DASVLN010000218         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_225344, whole genome shotgun sequence.
ACCESSION   DASVLN010000218 DASVLN010000000
VERSION     DASVLN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225344"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VF453_04970"
     CDS             complement(<1..1189)
                     /locus_tag="VF453_04970"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEX7687032.1"
                     /translation="MTAPPSSGRRSLVIMGIRGVPAAHGGFETFAARLAPWLVEHGWD
                     VTVYCEEPVRTPAPYESEWRGVRRVHIGFGPDRAINSIRFDWACIDHFLAERPRKPLV
                     LTLGYNTALFGLRLRAAGVTHVINMDGIEWARSKWGPIAKAWLYLNDWGGCLGASHLV
                     ADHPEIARHLSSRVDARRISMIPYGTDLVDHADPMPLAALGLAPGGFFSLIARPEPEN
                     SVLEIVRAFSARRRGLKLVVLGRYRPEAYPYHAAVMKAASDEVMFPGAIYDAEVLAAL
                     RQHSLAYLHGHQVGGTNPSLLEAMGAGNAVIAHDNRFNRWVAQDGALYFADAAGCAEA
                     IDRLAADPSLGARLGRGNRARAEGRFGWPLVLSQYEMTMAALHARATGVVDAPVPPLA
                     GHKD"
     gene            complement(1186..>1518)
                     /locus_tag="VF453_04975"
     CDS             complement(1186..>1518)
                     /locus_tag="VF453_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687033.1"
                     /translation="LGRLLGVRLQDDLAAELAQAAARGVALDFVFSRDDPGRVLLREE
                     AGRPGMRLQRDKRLQVCEVAHADHTFAGTRGRAGLYARLDCLLQPAAPMSPVPSATLP
                     PLPAAARP"
BASE COUNT          206 a          554 c          566 g          192 t
ORIGIN      
        1 cgtccttgtg cccggccagc ggcggcacgg gggcgtccac cacgcccgtg gcccgggcgt
       61 gcaaggccgc cattgtcatc tcgtattggg acagcaccag cggccagccg aatcggcctt
      121 cggcgcgcgc gcggttgccg cggcccaggc gcgcgcccag cgacgggtcc gccgccaggc
      181 ggtcgatcgc ctcggcgcag cccgcggcgt ccgcgaagta cagcgcgccg tcctgcgcga
      241 cccagcggtt gaagcggttg tcgtgcgcga tcaccgcgtt gcccgcgccc atcgcctcca
      301 gcaacgacgg attggtgccg ccgacctggt ggccgtgcag gtaggcgagc gaatgctggc
      361 gcagcgcggc gagcacctcg gcgtcgtaga tcgcgcccgg gaacatcacc tcgtcgctcg
      421 ccgccttcat cacggcggcg tggtacggat aggcctcggg ccggtaacgc ccgagcacca
      481 ccagcttcag cccgcgccgc ctggcgctga acgcgcgcac gatctcgagc accgagttct
      541 ccggctccgg ccgcgcgatc agcgagaaga agccgcccgg cgcgaggccc agcgccgcca
      601 gcggcatcgg gtcggcgtgg tccacgaggt cggtgccgta cgggatcatg ctgatgcgcc
      661 gcgcgtcgac ccggctcgag aggtggcgcg cgatctcggg atggtcggcc acgaggtgcg
      721 aggcgccgag gcagccgccc cagtcgttga ggtacagcca ggccttggcg atcggccccc
      781 acttgctgcg cgcccactcg atgccgtcca tgttgatgac gtgcgtgacg ccggccgcgc
      841 gcagccgcag gccgaacagc gccgtgttgt agcccagcgt cagcaccagc ggcttgcgcg
      901 ggcgctcggc gaggaagtgg tcgatgcagg cccagtcgaa gcggatgctg ttgatcgccc
      961 ggtcggggcc gaagccgatg tgcacgcgcc ggacgccgcg ccactcgctt tcgtacgggg
     1021 cgggcgtgcg caccggctcc tcgcagtaga ccgtgacgtc ccagccgtgt tccaccagcc
     1081 agggcgcgag tcgcgcggcg aaggtctcga atccgccatg cgccgcgggc accccgcgga
     1141 tgcccatgat cacgagcgac ctccggccgg acgacggcgg cgcggtcatg gccgggcggc
     1201 cgcgggcagc ggcgggaggg tggcggacgg gacgggcgac atgggggcgg cgggctgcag
     1261 caggcaatcc agtcgagcgt acagaccggc ccgcccgcgc gtcccggcga acgtgtggtc
     1321 cgcgtgcgcg acttcgcaca cttgcagccg tttgtcgcgt tgcagcctca tcccgggacg
     1381 gccggcctcc tcgcgcagca gcacgcggcc ggggtcgtcg cgcgagaaca cgaagtcgag
     1441 cgcgacgccg cgcgccgcgg cctgcgccag ttcggcggcg aggtcgtcct gcagccgcac
     1501 gcccagcagg cggccgag
//
LOCUS       DASVLN010000219         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_280012, whole genome shotgun sequence.
ACCESSION   DASVLN010000219 DASVLN010000000
VERSION     DASVLN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280012"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VF453_04980"
     CDS             <1..1321
                     /locus_tag="VF453_04980"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HEX7687034.1"
                     /translation="PAPAPVSATAVTAAFASTSADIPNPDRGFYVWGGSDFVTSFDLG
                     SIQAGFANGQRLALASISLAAYRTSDLPASFLSTLSSNLAAVRSAGMKVSLLFAYDFS
                     ASGADASAAQIKRHLEQLQPVLAANADVIPYMRAGFIGAWGEWHSSQSGNSCGYNSGS
                     TPCSTADANRAIVRDALLANVPSTTQIGFRYPADLQLWYPSGNAPARVGMHNDCFLAG
                     PTDTGTYTSSGQRPFAQTLTQRGAFGGETCEGGETPVRSSCSDILSEGAQYHLAWLNS
                     VYAPSVLNAWKSGGCYSQVSATMGYRIQVDRVTHESAVARGATLAVDVDLRNVGWARM
                     FVARKLVITLRNRSTGAVVVASGGDLSALPEQATSSSRITVSVPVPAGAATGMYDVLL
                     SAPDVFAATKGDARFAVRFANADQSSSGQVWSASAATLATGTAVQVE"
     gene            complement(1338..2516)
                     /locus_tag="VF453_04985"
     CDS             complement(1338..2516)
                     /locus_tag="VF453_04985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX7687035.1"
                     /translation="MTGRNVACAIRANVRGMSRWTPPPADTLRLMLVGDLMTGRGIDR
                     ILRWPLPAPPGLGAREARRQLREAERANGPVPLRSGPDYVWGDALEAMAQLDAPLRLA
                     SLGTAITSAATAWAGKDTHLRMNPRHVDCLAAGGITACALASDHLLDWDYPGLRETLA
                     ALSEAGIAGAGAGLDAASALSPAIMAIGPGARLLLFSWATPAAGIPAGWGATSTRPGI
                     ALLPGLGEADAREVKACVECHRKPGDLVVVSLHWGEREAAEVSAAQRAFAHRLVDLGI
                     VDVLHGHGARAPLPFEVYGDRLLLYACGDLIDDEEAMPVAEGQRADLGCLYVATLARR
                     DGRLRMLEIQPLRRRRFRLERADAEAWIWLGRRLGLPSEDRGAGDDAPEIPWCLRWDA
                     "
     gene            2587..>2822
                     /locus_tag="VF453_04990"
     CDS             2587..>2822
                     /locus_tag="VF453_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687036.1"
                     /translation="MEPQQRPYAAPHHSWSDRERLLLSLLLWAASRDAGGGGRDPMGR
                     LRLAVLLQSGAFLYDPRAHALRPCGAQDLRTRRDR"
BASE COUNT          369 a         1107 c          988 g          358 t
ORIGIN      
        1 gcccgccccg gcgccggtgt ccgcgaccgc cgtcaccgcc gccttcgcct ccacgagcgc
       61 cgacatcccc aacccggatc gcggcttcta cgtctggggc ggcagcgact tcgtcaccag
      121 cttcgacctc ggcagcatcc aggccggctt cgccaacggc cagcgcctgg cgctcgcgtc
      181 gatctccctg gcggcctacc gcacgtccga cctgccggcc tcgttcctgt ccacgctctc
      241 gtccaacctc gcggccgtgc gctcggcggg catgaaggtc tcgctgctgt tcgcctacga
      301 cttcagcgcc tccggcgccg acgccagcgc ggcgcagatc aagcgccacc tggaacagct
      361 gcagcccgtg ctggccgcca acgccgacgt catcccgtac atgcgcgcgg gcttcatcgg
      421 cgcgtggggg gagtggcact cgtcgcagtc ggggaactcc tgcggctaca actccggcag
      481 cacgccgtgc tcgaccgccg acgccaaccg cgcgatcgtg cgtgacgcgc tgctggccaa
      541 cgtcccgtcg acgacccaga tcggcttccg ctatccggcg gacctgcagc tctggtaccc
      601 gagcggcaat gcaccggccc gcgtcggcat gcacaacgac tgcttcctcg ccggaccgac
      661 cgacacgggc acgtacacct cgtcgggaca gcgtccgttc gcgcagacgc tgacccagcg
      721 cggcgcgttc ggcggcgaga cctgcgaggg cggcgagacg ccggtgcgca gcagctgctc
      781 cgacatcctg agcgagggcg cgcagtacca cctcgcctgg ctcaacagcg tctacgcgcc
      841 ctcggtgctg aacgcctgga agagcggcgg ctgctacagc caggtgtcgg cgacgatggg
      901 ctatcgcatc caggtcgacc gggtcaccca cgagagcgcc gtcgcgcgcg gcgcgacgct
      961 ggccgtcgac gtcgacctgc gcaacgtcgg ctgggcgcgg atgttcgtcg cgcgcaagct
     1021 ggtgatcacg ctgcgcaacc gctcgaccgg cgcggtggtc gtcgccagcg gcggcgacct
     1081 gagcgccctg ccggagcagg cgacgtcctc gagccgcatc accgtgagcg tgcccgtgcc
     1141 cgcgggcgcg gcgaccggga tgtacgacgt gctcctgagc gcgcccgacg tgttcgccgc
     1201 caccaagggc gacgcgcgct tcgccgtgcg cttcgccaat gccgaccagt cgtccagcgg
     1261 ccaggtctgg agcgcctcgg cggcgacgct ggcgacggga acggccgtgc aggtggagtg
     1321 acctgcgacg ggccgcctca ggcgtcccag cgcaggcacc aggggatctc cggggcgtcg
     1381 tccccggctc cccggtcctc gctgggcagc ccgagcctgc ggcccagcca gatccaggcc
     1441 tcggcgtccg cccgctccag gcggaaccgg cgacggcgca gcggctggat ctccagcatc
     1501 cgcagccgtc cgtcgcgccg cgcgagggtc gccacgtaca ggcagcccag gtcggcgcgc
     1561 tgtccctcgg cgaccggcat cgcctcctcg tcgtcgatga ggtcgccgca ggcgtacagc
     1621 aggagccgat cgccgtagac ctcgaagggc agcggcgccc gggcaccgtg gccgtgcagc
     1681 acgtccacga tgccgaggtc gaccaggcgg tgcgcgaacg cccgctgcgc cgccgacacc
     1741 tccgccgcct cgcgctcgcc ccagtgcagc gagacgacga ccaggtcgcc cggcttgcga
     1801 tggcattcaa cgcaggcctt gacctcgcgc gcgtcggcct cgccgagccc cggcagcagc
     1861 gcgatgccgg gccgcgtgct ggtggcgccc caacccgccg ggatgccggc ggcgggcgtc
     1921 gcccacgaaa acagcagcag ccgcgctccc gggccgatcg ccatgatcgc cggcgagagc
     1981 gcggacgcgg cgtccagtcc cgcccccgcg ccggcgatgc cggcctccga cagcgccgcc
     2041 agcgtctcgc gcaggccggg gtagtcccag tccagcaggt ggtcgctcgc cagcgcgcag
     2101 gcggtgatcc cgccggcggc gaggcagtcg acgtgccgcg ggttcatgcg caggtgcgtg
     2161 tccttgccgg cccatgccgt cgccgccgag gtgatggccg tgccgaggct ggcgaggcgc
     2221 agcggcgcgt ccagctgcgc catcgcctcg agcgcgtcgc cccagacgta gtccgggccg
     2281 ctgcgcagcg gcaccggccc gttcgcgcgc tcggcctcgc gaagctgccg acgcgcctcc
     2341 cgcgcaccga gcccgggcgg cgccggcagc ggccagcgga ggatccggtc gatgccgcga
     2401 ccggtcatca ggtcgcccac cagcatcagg cgcagcgtgt cggcgggcgg cggcgtccat
     2461 cgtgacatcc cgcgcacgtt ggcgcggatg gcgcaggcga cattgcgtcc agtcaatcgg
     2521 acggaggacg acgcgccgac catggcaccg ttgccgcgca cgccagcggc ccgggacgac
     2581 ctcatcatgg agccgcagca gcgaccgtac gccgcccctc accattcctg gagcgatcgc
     2641 gagcgcctgc tgctgtcgct cctgctttgg gccgcctcgc gcgacgccgg cggcggcggc
     2701 cgcgatccga tgggccggct ccggctcgcg gtgctgctgc aaagcggcgc cttcctctac
     2761 gatccgcgcg cgcacgcgct gcgcccctgc ggggcccagg acctgcgcac gcgccgcgat
     2821 cg
//
LOCUS       DASVLN010000220         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_386392, whole genome shotgun sequence.
ACCESSION   DASVLN010000220 DASVLN010000000
VERSION     DASVLN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386392"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..461
                     /locus_tag="VF453_04995"
     CDS             222..461
                     /locus_tag="VF453_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687037.1"
                     /translation="MTSAPRTYPDPKPARGAMLVRGVCGAALGVVMAVGIWIKAGGLG
                     WWPSVVVGLGACGTRAFGSMRHGDAFWFRVLRRRS"
     gene            723..1250
                     /locus_tag="VF453_05000"
     CDS             723..1250
                     /locus_tag="VF453_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687038.1"
                     /translation="MSAQIISNRISLSIPADEMQAIQDAIKVLQDKLLPHLVDLGPTD
                     RRTMPRMGAKTVDFVSKTLGSATAHPQIVPGFVDLGEFSRDLAAVGTLRALLQPLQQL
                     ADLVGDSLALSGSEAYTAALACYQSFKAASKLGVPGMTTIADDLAERFPGRPSRAGSP
                     PASNGGDASPADDPA"
     gene            1654..>2777
                     /locus_tag="VF453_05005"
     CDS             1654..>2777
                     /locus_tag="VF453_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687039.1"
                     /translation="MSPDLPTKKDVIDYLRNQQKRAEIDSKVDGVNIWVLYGAIALVV
                     WKIVEPFDPKDWASPVLVARLVALSILLDGLRSGVMGLATRGPEPLFRWPRRGDASRR
                     FFLARYTLVAAPFIATLALDDRDPVAFFLALGSFGGYVVFVLEALYDRFSTRRTGFRF
                     PPAVGQPVPAALLGIALGAAVVWHFAAAIVPSLAAITAGQVKACLLALTLYWLLVTLA
                     RRHARIRQLDWTYVLERDLLLGPKSVPAALTEIEYHASGVELPRVFDRYFEQVRDELA
                     ELGARIDAFNGRLAAAFAVPAEQTLERQMQHDELRRLVEEPLERLAATFGDFHAFQRV
                     GLRQRIPPHAPGLDAALAAVRDKQAQAEAVVDAARVEIDAT"
BASE COUNT          390 a         1026 c          903 g          458 t
ORIGIN      
        1 ctcctcgagg aactcgcgct tgcgcgtctt cttcgtcgac aggttcaggc caagtccgag
       61 ctgtttcatg ggcccgattt tcgtccggcg ccgctcttca cgctacgcgg ggccgcccgg
      121 agttttgcag actgtcccta accagcttgc cggtcagccg acgttcgcga tcgtcgggta
      181 atggccgaaa tctgctgccg aggttatggt accgctacac catgacgagc gccccccgca
      241 cctatcctga cccgaagcca gcgcgcggcg cgatgctggt tcgcggcgtt tgcggagcgg
      301 cgctcggcgt cgtcatggcc gtcggcatct ggatcaaggc aggcggcctc gggtggtggc
      361 cgtccgtcgt cgtggggctc ggcgcttgcg gcacgcgcgc cttcgggtcc atgcgccacg
      421 gggatgcctt ctggttccgg gtgctccggc gtcgctcgta gcccgtcgaa gctggcgcgg
      481 ccccatcgac cgcttccgac cggtcatgcg cgaaggcctc ttggcccatg gaacgctgga
      541 cggacgcaag gcgtccggcg atcttcgtga cgacgcgggt gccgacacgg cgggacgcag
      601 ccacccccta agttcgggcc ggcccctagc ctgaagggtt ggatacacca ccccgtatca
      661 caacaatcgc ccaccgcccg gcacggtgcc ccgcggaaac tccatcaagg aaggacgatt
      721 cgatgagtgc ccagatcatc agcaaccgca tctcgctcag catcccggcg gacgagatgc
      781 aggccatcca ggacgcgatc aaggtgctgc aggacaagct gctgccgcac ctcgtcgacc
      841 tcgggccgac cgaccgccgc acgatgccgc gcatgggtgc gaagaccgtc gacttcgtct
      901 cgaagaccct gggctccgcg accgcgcatc cgcagatcgt cccgggcttc gtcgacctgg
      961 gggagttctc gcgcgacctc gccgccgtcg gcacgctgcg ggcgctgctg cagccgctgc
     1021 agcaactggc cgacctggtc ggcgacagcc tcgcgctctc gggcagcgag gcctacaccg
     1081 ccgccctcgc ctgctaccag tcgttcaagg ccgcctccaa gctgggcgtc ccgggcatga
     1141 ccacgatcgc cgacgatctc gccgagcgct tccccggacg cccgtcgcgc gccggttcgc
     1201 cgccggcatc gaacggtggc gacgcctcgc ccgccgacga cccggcctga tccgctgtcg
     1261 ccgccggcgt agtcgccggc aatggaatcg gaaacaccgg ccctggcttc cagaacatcg
     1321 gccgtgtatt ccccggaaac gacgccgccc cgaaaagggg cggcttttca ttccggaaca
     1381 ccggcgccgc tgtttcctat tccaacgtcg gcgtttccga ttgcaagtcg ccgctggcgg
     1441 gaagtcggaa ttgcgatcct ggacacctcc ggccggttcc gggatgccgt cattggtgtt
     1501 tcggaccgca agcccgatgt tttcaaacgc ccggccgatg ttctccaatg catcccgtcc
     1561 ccgcgcgaca atgctagagt ccccgccaat tccaatttcc gacccgcgcg cgcccatccg
     1621 caccggtgcg tgcgcgaccc ctgggaggtc gccgtgagcc cggacttgcc gacgaagaag
     1681 gacgtgatcg actacctgcg caaccagcag aagcgcgcgg agatcgattc caaggtcgac
     1741 ggcgtcaaca tctgggtgct gtacggcgcc atcgcgctcg tcgtctggaa gatcgtcgag
     1801 cccttcgacc cgaaggactg ggcgtcgccg gtgctggtgg cgcggctggt cgcgctctcg
     1861 atcctgctgg acggcctgcg ctccggcgtc atgggcctgg cgacgcgcgg gccggaaccg
     1921 ctgttccggt ggccgcgccg cggcgacgcc tcgcgccgct tcttcctcgc ccgctacacc
     1981 ctcgtcgcgg ccccgttcat cgcgaccttg gcactggacg accgcgaccc ggtcgcgttc
     2041 ttcctcgcgc tgggcagctt cggcggctat gtcgtgttcg tcctcgaagc cttgtacgac
     2101 cgcttctcga cacgccggac cggcttccgc ttcccgccgg ccgtcggcca acccgtgccg
     2161 gccgccctgc tcggaatcgc cctcggcgcc gcggtcgtct ggcacttcgc ggccgccatc
     2221 gtgccctcgc tggcggccat cacggccgga caggtcaagg cctgcctgct cgcgctgacg
     2281 ctgtactggc tgctcgtcac gctggcgcgc cggcacgccc gcatccgcca gctcgattgg
     2341 acgtacgtgc tcgaacgcga cctgctgctg gggccgaagt ccgtgcccgc ggccctgacg
     2401 gagatcgaat accacgcgtc cggcgtcgag ctgccgcgcg tcttcgaccg ctatttcgag
     2461 caggtgcgcg acgaactcgc cgagctcggg gcccgcatcg acgcgttcaa cgggaggctg
     2521 gccgccgcgt tcgcggtgcc cgccgagcag acgctggagc gccagatgca gcacgacgag
     2581 ctgcgacggc ttgtcgagga gccgctggaa cgcctggccg cgacctttgg cgacttccac
     2641 gcgttccagc gcgtcgggct gcgccagcgc atccctcccc acgcgccggg gctggacgcc
     2701 gcgctggccg cggtgcgcga caagcaggcc caggcggagg ccgtcgtcga cgcggcgcgc
     2761 gtggagatcg acgccac
//
LOCUS       DASVLN010000221         1900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_495158, whole genome shotgun sequence.
ACCESSION   DASVLN010000221 DASVLN010000000
VERSION     DASVLN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495158"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VF453_05010"
     CDS             <1..157
                     /locus_tag="VF453_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7687040.1"
                     /translation="ASQRTVQRALVELEAAGRVRSIGRARAQRWLAPPLAGFTTILLL
                     PGALPRG"
     gene            201..914
                     /locus_tag="VF453_05015"
     CDS             201..914
                     /locus_tag="VF453_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687041.1"
                     /translation="MPIPRSAADAALPIAEPAAEPAEVLRELGPFPGVTQVAGVTRDG
                     TQLWAATPAGLMALDPVTGRAGRVIEQPADAGTAFDGRHLWQVAEARIDKIDPATGEV
                     VASIPAPGQGRDSGLAWAEGSLWVGQYRDRRIVQVDPADGRVLRTIESNRFVTGVTWA
                     GDELWHGTWEGDESELRRVDPASGAVLERLAMPAGVGVSGLESDGADVFYCGGGHSGR
                     IRVVRKPARGRTPAVPTSS"
     gene            1029..1820
                     /locus_tag="VF453_05020"
     CDS             1029..1820
                     /locus_tag="VF453_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7687042.1"
                     /translation="MSNALVDNDTTSRLAASTAPHAVVSHARWVEARRALLAREKALQ
                     RQSDQLARERQALPWERVEKDYVFETAAGPRRLGELFDGCSQLIVQHVMFAPGDDAAC
                     KHCAFMSDHVDPMLPHLARRDTAFIAIARAPVAALERFGQRMGWRLRWASSGGNDFNY
                     DYGVSFTRAELARGHVTYNYGEVAFPHQDAPGISVFARAADGTVFHTYSTYFRGVERM
                     MLTYQFLDIVPKGRDEAETGPMGWVRLHDEYESADGAAAHACCGD"
BASE COUNT          241 a          739 c          678 g          242 t
ORIGIN      
        1 cgcgagccag cgcacggtgc agcgggcgct ggtcgagctc gaggccgccg gccgcgtgcg
       61 ctcgatcggc cgcgcccggg cccagcgctg gctggcgccg ccgctggccg gattcacgac
      121 gatcttgtta ctccccggcg cgcttccgcg cgggtagatt gcatccaacg cgccacggat
      181 tgcgcgacct ggagaccgag atgcccatcc cccgttccgc cgccgacgcc gccctgccca
      241 tcgccgagcc ggcggccgag cccgccgagg tgctgcgcga gctgggcccc tttcccggcg
      301 tcacgcaggt ggccggcgtg acgcgcgacg gcacgcagct gtgggccgcg accccggccg
      361 ggctgatggc gctcgacccc gtcaccgggc gcgccgggcg cgtgatcgag cagcccgccg
      421 acgccggcac cgcgttcgac ggccgccatc tctggcaggt cgcggaggcg cgcatcgaca
      481 agatcgaccc ggccacgggc gaggtcgtcg cgtcgatccc cgcgccgggc cagggccgcg
      541 actcgggcct cgcctgggcc gagggcagcc tgtgggtcgg ccagtaccgc gatcgtcgca
      601 tcgtccaggt cgatcccgcc gacggccgcg tgctgcgcac gatcgagtcg aaccgcttcg
      661 tcaccggcgt gacatgggcc ggcgacgagc tctggcacgg cacctgggag ggcgacgaga
      721 gcgagctgcg ccgcgtcgat cccgccagcg gcgcggtgct cgagcggctg gcgatgccgg
      781 ccggcgtcgg cgtcagcggg ctcgagtccg acggcgcgga cgtcttctac tgcggcggcg
      841 gccacagcgg gcgcatccgc gtggtgcgca agccggcgcg cggccgcacg cccgcggtgc
      901 cgacgtcgag ctgacgcccg tgtcctgacg cggccgccgc gtccccgccg attttcctcg
      961 cgctcgcgac ggcctcatcc cggccgcgca ggcgcagcct tgccccgccg tccaaccagg
     1021 agcccaccat gagcaacgcc ctcgtcgaca acgacaccac ttcgcgcctc gccgcatcca
     1081 ccgcgccgca cgccgtcgtc tcccacgccc gctgggtcga ggcccggcgc gcgctgctgg
     1141 cgcgcgagaa ggcgctgcag cgccagtccg accagctcgc gcgcgagcgc caggccctgc
     1201 cctgggagcg cgtggaaaag gactacgtct tcgagaccgc cgccggcccg cgccgcctgg
     1261 gcgagctgtt cgacggctgc agccagctga tcgtgcagca cgtgatgttc gcgccgggcg
     1321 acgacgcggc ctgcaagcac tgcgcgttca tgtccgacca tgtcgacccg atgctgccgc
     1381 acctggcccg ccgcgacacg gcgttcatcg cgatcgcgcg cgcccccgtc gcggcgctcg
     1441 agcgcttcgg acagcgcatg gggtggcgct tgcgctgggc ctcgtccggc ggcaacgact
     1501 tcaattacga ctacggcgtc tccttcacgc gcgcggagct cgcacgcggc cacgtcacct
     1561 acaactacgg cgaggtcgcg ttcccgcacc aggacgcgcc gggcatcagc gtgttcgcgc
     1621 gggcggcgga cggcaccgtc ttccatacct actccacgta tttccgcggc gtcgagcgga
     1681 tgatgctgac ctaccagttc ctcgacatcg tgcccaaggg ccgcgacgag gcggagaccg
     1741 gcccgatggg ctgggtgcgc ctgcacgacg agtacgagtc cgccgacggc gccgcggcgc
     1801 acgcctgctg cggcgactga agccggccgc gaggaacgac gccatggccg acggctggac
     1861 ctggctggcg ctcgcgggcg ccggcgcact gcacgggctc
//
LOCUS       DASVLN010000222         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_333447, whole genome shotgun sequence.
ACCESSION   DASVLN010000222 DASVLN010000000
VERSION     DASVLN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333447"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..678
                     /locus_tag="VF453_05025"
                     /pseudo
     CDS             1..678
                     /locus_tag="VF453_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265184.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            682..1143
                     /gene="aroQ"
                     /locus_tag="VF453_05030"
     CDS             682..1143
                     /gene="aroQ"
                     /locus_tag="VF453_05030"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma subclass chorismate mutase AroQ"
                     /protein_id="HEX7687043.1"
                     /translation="METLRRLIDARLLLAPDVAQFKWNHHIPIEDREREAQIVAQLGG
                     QAAALGVPREAAERFFRAQIEASKIAQNELFQGWDVFKHGQFPDAPDLATVTRPKIDT
                     LNEQLLHALADNWPVLSDPRRKADVERALRPMAAEDVSAKAVAEALSPLVR"
     gene            complement(1151..1387)
                     /locus_tag="VF453_05035"
     CDS             complement(1151..1387)
                     /locus_tag="VF453_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HEX7687044.1"
                     /translation="MDLANLVQMANRIGDFFAAQPDRAEALQGVAEHIRKFWEPRMRR
                     ELLAAIDAGEAAGLSEIVAAAIAAHRAALEPKAA"
     gene            complement(1394..>1520)
                     /locus_tag="VF453_05040"
     CDS             complement(1394..>1520)
                     /locus_tag="VF453_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX7687045.1"
                     /translation="APTARAVDVARRAGLALAGFARRDDLVLYAGAGRFERPSSH"
BASE COUNT          222 a          551 c          543 g          204 t
ORIGIN      
        1 ggcgtgctgc gcgtgggcac gaccggcgac tacaagccgt tcagcctgcg caccggcggc
       61 aacgcgttcg tcgggctgga catcgcgctg gccgccgacc tcgcgaagtc gctgggcgtg
      121 aagctcgagg tcgtgccgac gacctgggcg acgctgatgc ccgacttcgt cgagggccgc
      181 ttcgacctcg cgctgggcgg cgtctcgatc acgctggagc gccagcgcaa ggcgtacttc
      241 tcgatcccct acctgcgcga cggcaagacg cccatcgtgc gctgcgacga catggcccgc
      301 taccagaccc tcgcgcagat cgaccggccc gaggtgcggc tgatcgtgaa cccgggaggc
      361 accaacgagc gcttcgcccg tgcgcacgcg ccgcacgcga cgatctcggt gtggccggac
      421 aacgtgacca tcttcgaccg catcgccaac gcggacgccg acgtcatgat cacggacgcc
      481 atcgaggccc ggttgcaggc gcgcctccat cccaagctgt gcgccgtgca tccggacgcg
      541 ccgttcgact tctcggagaa ggccgtcctg ctgccgcgcg acgagccgct gaaggcctac
      601 gtcgatcagt ggctgcacca gctgatcgcc tccggcacct tcgaccggat gaacgagcag
      661 tggctcgcct atccgtgggg catggagacg ctgcgccggc tgatcgacgc gcgcttgctg
      721 ctggcgccgg acgtcgcgca gttcaagtgg aaccaccaca tcccgatcga ggaccgcgag
      781 cgcgaggcgc agatcgtcgc gcagctcggc ggacaggccg ccgcgctggg cgtgccgcgc
      841 gaggccgcgg agcgcttctt ccgcgcgcag atcgaggcgt cgaagatcgc gcagaacgag
      901 ctgttccagg gctgggacgt cttcaagcac ggccagttcc ccgacgcgcc cgacctcgcg
      961 accgtcacgc gcccgaagat cgacacgctc aacgagcagc tgctgcacgc cctcgcggac
     1021 aactggcccg tcctgtccga cccgcgccgc aaggccgacg tcgagcgcgc gctgcgcccg
     1081 atggcggcgg aggacgtcag cgcgaaggcg gtcgcggagg cgctctcgcc gctggtgcgc
     1141 tgagcgcgga tcaggccgcc ttcggctcca gcgccgcccg gtgcgccgcg atcgcggcgg
     1201 cgacgatctc gctgaggccg gcggcctcgc cggcgtcgat cgctgcgagc agctcgcggc
     1261 gcatgcgcgg ctcccagaac ttgcggatgt gctcggcgac cccctgcagc gcctccgcgc
     1321 ggtcgggctg ggcggcgaag aagtcgccga tgcggttggc catctggacg aggttggcga
     1381 gatccatgtc ggttcagtgg ctggaaggcc gttcgaagcg cccggcgccg gcgtagagca
     1441 cgaggtcgtc gcggcgcgcg aagccggcga gcgccaggcc ggcgcggcgc gcgacgtcga
     1501 ccgcacgcgc cgtcggcgcc
//
LOCUS       DASVLN010000223         6668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_495184, whole genome shotgun sequence.
ACCESSION   DASVLN010000223 DASVLN010000000
VERSION     DASVLN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495184"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1494
                     /locus_tag="VF453_05045"
     CDS             184..1494
                     /locus_tag="VF453_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX7687046.1"
                     /translation="MPTAPPADYCAIDFGTSNSAVAVPAGDAMALVPLEPEHGERGRT
                     MPTAVFYLAEGPGLQNLPKLYGRAAVAAYVEGTDGRLMRSMKSALGSALMEQHTDVGA
                     GRSVAFGDVIASYLLHLRRAAQAHAGREIERVVLGRPVFFVDDDATRDAAAQAGLEAA
                     ARSVGFREVHFQYEPIAAAFHYEQQVDREQVVIVADIGGGTSDFSVVRVGPERIGRAD
                     RRDDILASHGVHVAGTDFDRHVELARILPALGYGAYGPPRAGRAPLEVPSRIYFDLAT
                     WHLINLCYRPQRLMEIASMRDDYADPRHHARLTTVLRERLGHALAGLAEEAKIAVAQG
                     GAHAVDLFEVERALEVEVTEAQVVEAVNHDLGRILQAAGDTARLAGLHADQIDALYFT
                     GGSTGLRLLAERLAAAFPQARPVHGDRFASVATGLGLDARRRFG"
     gene            1750..2367
                     /locus_tag="VF453_05050"
     CDS             1750..2367
                     /locus_tag="VF453_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7687047.1"
                     /translation="MKLIGSLTSAYVRKVRIVLAEKKLDYNFVLEDVWGSDAILASNP
                     LGKVPCLVMEGAEAVFDSRVIVEYVDTLSPVGKLIPSSGRERVEVRTWEALADGLIDA
                     AVVARLEKTWPGRTDGQRCDAWVDRQMVRIAAAIKSISQGLGDKPFCAGTHFSLADIA
                     VGCALGYLDFRFPEIAWRADHPNLARLADKLAQRQSFADTVPREA"
     gene            2448..3737
                     /locus_tag="VF453_05055"
     CDS             2448..3737
                     /locus_tag="VF453_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX7687048.1"
                     /translation="MGSQKIEHYTHPAHFEPLLPGQHKMGPLLERASDLTRAASRLTL
                     PAGQLPMRQLGELLRRMNSYYTNRLEGEHTRPSEIERALHDDYSRSADLARRQRLAVA
                     HIRTELSCEAELDGWLAIEARLPRDSAFSSIAMDSVLAALYGPEALQWLHRELFSELE
                     AGDLRLTDGSSMVPGEFRERQVSVGRHEAPLHTAVPGFLGRWNDVYARARRGEQSIVA
                     LAAAHHRLTWIHPFPDGNGRVARLHSHLMLRSMGLTHGLWSPLRGFARTEERYKATLA
                     AADEHRRGDLDGRGNLTEAGLIDWIDYCLDTFIDQANFMGEQLNVATMRERIAAALAF
                     ESSIRRSGVRSEALIPLHYLFATAGSIPRAAFKTMTGLGDRTATSLVSALVERGFLES
                     DTAYGDVRFGIPDHALRFYFPALWPEAEQEQASRGDA"
     gene            3813..4658
                     /locus_tag="VF453_05060"
     CDS             3813..4658
                     /locus_tag="VF453_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687049.1"
                     /translation="MRAREAHCGRCPIARRKPMHRRTLVGALPALAILKSFAQAVDGA
                     ASQPAGAAAGRKYVCLSLVADEIQYVQSVEPAVGHNLQRNEVTAEPAANLHLDEAALV
                     AMNEALDRSSAKGAPRSFLIGSDPAFFEDQYEWFSGDRATLPPKLAKSIDGEKATHLL
                     LLTKSRSDAADIKTVDFGVNVGRGHVSGVGIYGDPGRGGVIGGGSQDSQQVQLRAPYF
                     GLAPFASVRLSLVELASGRVLAHKTAFRARALARSSESSRFALLQDLIQQAVLETLPA
                     LVDAS"
     gene            complement(4704..6416)
                     /locus_tag="VF453_05065"
     CDS             complement(4704..6416)
                     /locus_tag="VF453_05065"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX7687050.1"
                     /translation="MPTWLLSLDRFFPIRYLTFLAFAVMMLLGAFTEVAFHTGGGWAI
                     VGLAGVVTGLRDVRQKRHSILRNYPVIGHLRFFLEFIRPEMRQYFIEGDNEAAPFSRQ
                     QRSLVYQRAKGDPDKRPLGTQLDVQAEGYEWINHSLQPSRVASHDFRVTIGANCAQPY
                     DASIFNISAMSFGALSGAAIRALNGGAKMGNFAHDTGEGSISVHHRAQGGDLIWEVAS
                     GYFGCRNADGTFNAELFAQHAREPQVKMIELKLSQGAKPGHGGVLPGPKVTKEIADAR
                     GVEQGVDCISPASHSAFSTPVEMLQFIARLRELSGGKPTGFKFCVGHPWEWFAIAKAM
                     LETGLLPDFIVVDGAEGGTGAAPLEFTDHVGAPLQEGLLLVHNTLVGLNLRDRIRIGC
                     AGKVVSAFDIARMMALGANWCNAARGFMFALGCIQAQHCHTDHCPTGVATQDPQRQKA
                     LVVPAKIERVYNFHRHTLEALRELVQAAGLQHPNEITANHVVRRVAGDKVKLLANHLE
                     FVAPGVLLKAMRGEADWPHNVYKVYWPLARANSFAAQAAHAQPDGLKVPMAPAGQATL
                     DLVG"
BASE COUNT          969 a         2387 c         2313 g          999 t
ORIGIN      
        1 gaggcgctcg cctggcggcc gcccgcgccc gggcgctacg tgctgcgcgt cgtcgacgac
       61 cagggccgcg ccgacgtgcg cgcgctcgag gtccgcttcc agtcgcccgg cgcgccgtga
      121 tttgacgccg cgcgccggcg ccgacacaat cgcgccccct tgccgttcca accgcccgcc
      181 gcgatgccga ccgccccgcc cgccgactac tgcgccatcg acttcggcac ctccaactcc
      241 gccgtcgccg tcccggccgg ggacgcgatg gcgctcgtgc cgctggagcc cgagcacggc
      301 gaacgcggcc gcaccatgcc cacggccgtg ttctacctgg ccgagggtcc ggggctgcag
      361 aacctgccca agctctacgg ccgcgccgcg gtggccgcct atgtcgaggg caccgacggc
      421 cgcctgatgc gctcgatgaa gagcgcgctc ggctccgcgc tcatggaaca gcacaccgac
      481 gtgggcgccg gccgctcggt cgcgttcggc gacgtcatcg cgagctacct gctgcacctg
      541 cggcgcgccg cgcaggcgca cgcggggcgc gagatcgagc gcgtcgtcct cggccggccg
      601 gtgttcttcg tcgacgacga cgccacccgc gacgccgcgg cccaggccgg gctcgaggcc
      661 gcggcgcgct cggtcggctt ccgcgaggtg cacttccagt acgagccgat tgccgcggcg
      721 ttccactacg agcagcaggt cgatcgcgag caggtcgtca tcgtcgcgga catcggcggc
      781 ggcacgtccg acttctcggt cgtgcgcgtc ggccccgagc gcatcggccg cgccgaccgc
      841 cgcgacgaca tcctcgcgag ccacggcgtg catgtcgccg gcaccgactt cgaccgccac
      901 gtcgagctcg cgcgcatcct gccggcgctc ggctacggcg cctacggccc gccgcgcgcg
      961 ggccgcgcgc cgctggaggt gcccagccgc atctacttcg acctcgccac ctggcacctg
     1021 atcaacctct gctaccggcc gcagcggctc atggagatcg cctccatgcg cgacgactac
     1081 gccgacccgc gccaccacgc gcggctgacc accgtgctgc gcgagcgcct gggccacgcg
     1141 ctggccggcc tcgcggagga ggccaagatc gcggtcgcgc agggcggcgc gcacgcggtc
     1201 gacctgttcg aggtcgagcg cgcgctggag gtcgaggtga ccgaggcgca ggtggtcgag
     1261 gcggtgaacc acgacctcgg ccgcatcctg caggccgccg gcgacacggc gcgcctcgcc
     1321 ggcctccatg ccgaccagat cgacgcgctg tacttcaccg gcggctcgac cggcctgcgc
     1381 ctgctcgccg agcgcctggc cgccgcgttc ccgcaggcgc ggccggtgca cggcgaccgc
     1441 ttcgccagcg tcgcgacggg cctcgggctc gacgcgcggc gccgcttcgg ctagccgcga
     1501 ccacggtcgc gttgcatcgc ggagacagcc gccccctgct tgggggatgt cgcgcggcgc
     1561 ggcttcgtgg tctgatcgcc gaccgccact gcgggaaggc cgggcgcggc gcggcatccg
     1621 ggcaccgccg tggtgcggcg agcccgcccg ccccgggcga accatcgcct cccgggtcgc
     1681 cgccgcgagg catttccgcc agggcgcttc gatagaatga agcgcaagcc ccaaggtctc
     1741 cttcgtcaca tgaaactcat cggatctctc accagcgcct acgttcgcaa ggtgcgcatc
     1801 gtgctggcgg agaagaagct ggactacaac ttcgtgctcg aggatgtctg gggcagcgac
     1861 gccatcctcg cctccaaccc gctcggcaag gtgccgtgcc tcgtgatgga aggcgcggag
     1921 gcggtgttcg actcgcgcgt gatcgtcgag tacgtcgaca cgctgtcgcc ggtgggcaag
     1981 ctgattccgt cgtcggggcg cgagcgcgtc gaggtgcgca cgtgggaggc gctcgccgac
     2041 ggcctgatcg acgccgccgt cgtcgcgcgc ctcgagaaga cctggcccgg tcgcaccgac
     2101 ggccagcgct gcgacgcctg ggtcgaccgc cagatggtgc gcatcgccgc ggcgatcaag
     2161 tcgatcagcc agggcctcgg cgacaagccc ttctgcgcgg gcacgcactt ctcgctcgcc
     2221 gacatcgcgg tcggctgcgc cctcggctac ctggacttcc gcttcccgga gatcgcctgg
     2281 cgcgccgacc accccaacct cgcccgcctg gccgacaagc tcgcgcagcg ccagagcttc
     2341 gcggacacgg tgccgcggga agcgtgaatc ccgcatccga tctcgcaaaa atggcagcct
     2401 cgagctgcca ttttcatagg cggatcgcga gatctacaat ctcgcaaatg ggatcgcaga
     2461 aaatcgagca ttacacccat ccggcccact tcgagccgct gcttccgggc cagcacaaga
     2521 tgggcccgtt gctcgagcgc gcgagcgacc tgacgcgagc cgcctcgcgc ttgacgctgc
     2581 cggcaggcca actcccgatg cgacagctgg gcgaattgct ccggcggatg aactcgtact
     2641 acacgaaccg gctggaaggc gagcacaccc ggccgagcga gatcgagcgc gccctgcatg
     2701 acgactattc gcgcagtgcc gacttggcgc ggcgccagcg gctggcggtc gcgcatatcc
     2761 gcaccgagct gtcatgcgaa gccgagctgg atggctggct tgccatcgag gctcgcttgc
     2821 cgcgggattc ggcgttttct tccattgcca tggactcggt actggccgcg ctatacggcc
     2881 cggaagcctt gcaatggctg cacagggagt tgttctccga actcgaggcc ggcgatttgc
     2941 ggctgacgga cggctcgtcg atggtgcctg gcgagttcag ggaacgccag gtcagcgtcg
     3001 gccgccatga agcgccgttg cacacggcgg tacctggctt cctcggtcgc tggaatgacg
     3061 tgtatgcgcg agcccgaagg ggcgagcagt cgatcgtcgc cctcgcggcc gcccatcacc
     3121 gactgacctg gattcacccg ttccccgatg gcaacggcag ggtggccagg ctccattcac
     3181 acttgatgtt gcgctccatg ggactcacgc acgggctctg gtctccgttg cgcggatttg
     3241 cccgcacgga ggaacgctac aaggccaccc tggctgctgc cgacgagcac cgccgcggcg
     3301 acctcgacgg ccgcggaaac ctcaccgaag ccgggttgat cgactggatc gactactgcc
     3361 tggacacctt catcgatcaa gccaacttca tgggtgaaca gctgaatgtg gccacgatgc
     3421 gtgaacggat cgccgcggcc ctggcgttcg aatccagcat ccgcagatcc ggcgttcgca
     3481 gcgaggcact cattccgttg cactatttgt tcgcgacagc gggatcgatc ccgcgggcgg
     3541 cattcaagac gatgaccggc ctgggcgacc ggaccgcgac ctcgctggtg tccgcgctgg
     3601 tcgaacgggg cttcctggaa agcgacacag cctacggcga cgttcgcttc ggcatccccg
     3661 accacgcgct acgtttctac ttcccggcgc tgtggccgga agcggaacag gagcaggcga
     3721 gccgcggcga tgcctgaccg tccgggaaaa cgccccagtg atcggctggc ggcggtttcg
     3781 tagaattgcc cacgggacga tgtggacgga gcatgcgggc gcgcgaggcg cactgcggcc
     3841 ggtgccccat cgcgaggagg aaacccatgc atcgcagaac cctggtcggc gcgctgccgg
     3901 ccctggccat cctgaaatcg ttcgcgcagg cggtcgacgg cgccgcctcg cagccggccg
     3961 gcgccgcggc ggggcgcaag tacgtgtgcc tgtcgctggt cgccgacgag atccagtacg
     4021 ttcaatcggt cgagccggcc gtcggccaca acctgcagcg caatgaggtg actgccgagc
     4081 ctgccgcgaa tctgcatctg gacgaggccg cgctcgtcgc gatgaacgaa gcgctcgacc
     4141 gcagctcggc caagggcgcg ccgcgcagct tcctgatcgg cagcgacccg gccttcttcg
     4201 aggatcagta cgagtggttc tcgggcgatc gggcgacact gccgcccaag ctcgcgaaat
     4261 cgatcgacgg cgagaaggcg acccacctcc tgttgctgac caagtcccgc tcggacgcgg
     4321 cggacatcaa gacggtcgac ttcggcgtga acgtgggtcg cgggcacgtg tcgggcgtgg
     4381 gcatctacgg cgatcctggc cgcggcggcg tcatcggcgg cggctcccag gacagccagc
     4441 aggttcaatt gcgggcgccg tacttcggtc tggccccctt cgcgagcgtg cggctgtcgc
     4501 tcgtggagct cgcgtcgggc cgggtgctcg cacacaagac cgccttccga gcgcgcgcgc
     4561 tcgctcgctc cagcgaatcc tcgcgcttcg cgctgcttca ggacctgatc cagcaggccg
     4621 tgctcgagac gctgccagcc ctcgtcgacg ccagctgagg tcgcgcgagc cgaatacgct
     4681 gctgggtatc agacccgccg acctcagccc accaggtcca gcgtcgcctg ccccgccggc
     4741 gccatcggca ccttgagccc gtccggctgc gcgtgcgcgg cctgggcggc gaagctgttg
     4801 gcgcgcgcga gcggccagta gaccttgtag acgttgtgcg gccaatcggc ctcgccgcgc
     4861 atcgccttca gcagcacgcc gggggccacg aactcgaggt ggttggcgag cagcttgacc
     4921 ttgtcgccgg cgacgcggcg caccacgtgg ttggcggtga tctcgttggg atgctgcagc
     4981 cccgcggcct gcacgagctc gcgcagcgcc tccagggtgt ggcggtggaa gttgtagacg
     5041 cgctcgatct tggccggcac caccagcgcc ttctgccgct gcggatcctg cgtggcgacg
     5101 ccggtcgggc agtggtcggt gtggcagtgc tgggcctgga tgcagccgag cgcgaacatg
     5161 aagccgcgcg ccgcgttgca ccagttcgcg ccgagcgcca tcatgcgcgc gatgtcgaac
     5221 gcgctgacga ccttgcccgc gcagccgatc ctgatccggt cgcgcaggtt cagacccacc
     5281 agcgtgttgt gcaccagcag caggccttcc tgcagcggcg cgccgacgtg gtcggtgaac
     5341 tcgagcggcg ccgcgcccgt gccgccctcg gcgccgtcga cgacgatgaa gtcgggcagc
     5401 aggccggtct cgagcatcgc cttggcgatc gcgaaccatt cccacggatg gccgacgcag
     5461 aacttgaagc cggtgggctt gccgcccgag agctcgcgca ggcgggcgat gaactgcagc
     5521 atctcgaccg gcgtcgagaa cgcgctgtgg ctcgcgggcg agatgcagtc cacgccctgc
     5581 tcgacgccgc gcgcgtcggc gatctccttc gtgaccttgg ggccgggcag cacaccgccg
     5641 tggccgggct tggcgccctg gctcagcttg agctcgatca tcttgacctg cggctcgcgc
     5701 gcgtgctgcg cgaacagctc ggcgttgaac gtgccgtccg cgttgcggca gccgaagtag
     5761 cccgacgcga cctcccagat caggtcgccg ccctgcgccc ggtggtggac gctgatcgag
     5821 ccctcgccgg tgtcgtgcgc gaagttgccc atcttcgcgc cgccgttgag cgcccggatc
     5881 gccgcgccgc tcaaggcgcc gaagctcatc gcgctgatgt tgaagatgct cgcgtcgtag
     5941 ggctgcgcgc agttcgcgcc gatcgtcacg cggaagtcgt gcgaggccac gcgcgacggc
     6001 tgcagcgagt ggttgatcca ctcgtagccc tcggcctgca cgtccagctg cgtgcccagc
     6061 gggcgcttgt cgggatcgcc cttggcacgt tggtacacga gagagcgctg ctggcgcgag
     6121 aacggcgccg cctcgttgtc gccctcgatg aagtactggc gcatctcggg gcggatgaac
     6181 tcgaggaaga agcgcaggtg gccgatcacc gggtagttgc gcaggatcga gtggcgcttc
     6241 tggcgcacgt cgcgcaggcc cgtgaccacg cccgcgaggc cgacgatcgc ccacccgccg
     6301 ccggtgtgga aggcgacctc ggtgaacgcg cccagcagca tcatcaccgc gaaggcgagg
     6361 aaggtgaggt agcggatggg gaagaagcgg tcgagcgaca gcagccaggt gggcatttgg
     6421 atctcgcgtc gtggtggtgc cggggctcgt cgcccccttc gtcatcttcg gcggcccggg
     6481 cgccgacttg atgacgccgg cgcgacggga cgcacgggtg ccgcccgcgg gcggcgatcc
     6541 agctgcaatg tactgccgcg cagccggcgg cgggccgcag gacttccccg agcgtgccag
     6601 cgggtacgtc agcccagcgc gagcgtcgcg cgcacggcgc cggcgcggtc ggcccgggca
     6661 ctgaggcc
//
LOCUS       DASVLN010000224         4906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_280129, whole genome shotgun sequence.
ACCESSION   DASVLN010000224 DASVLN010000000
VERSION     DASVLN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280129"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(493..1032)
                     /gene="flhC"
                     /locus_tag="VF453_05070"
     CDS             complement(493..1032)
                     /gene="flhC"
                     /locus_tag="VF453_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HEX7687051.1"
                     /translation="MRVKSILTEAKQIERAVTLIKLGARLQVLESETDLSYERLLRLY
                     KEVSGKSPSKGQLPFSTDWFMTWQPNIHASLFLNIHEYLNKAAAMEEIDTVIKAYQLY
                     QEQTEAQGLEPLLSITRAWRLVKFVDNGMLTMTKCTKCGGHFVTHPHEMARHFVCGLC
                     NPPARAGKGKAAGALSLAH"
     gene            complement(1087..1410)
                     /gene="flhD"
                     /locus_tag="VF453_05075"
     CDS             complement(1087..1410)
                     /gene="flhD"
                     /locus_tag="VF453_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HEX7687052.1"
                     /translation="METNQILAEIREANLSYMMLAQSLIRSDREQALFRLGISEETAN
                     MIQALTPAQMMKIASGNTLLCRFRMDDDLIWNLITSHGKSAANETVSRLHASILMAGR
                     HQEAA"
     gene            complement(1732..2349)
                     /locus_tag="VF453_05080"
     CDS             complement(1732..2349)
                     /locus_tag="VF453_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687053.1"
                     /translation="MTLSCISPGATLGANVRIGRFCIVEEDVVLADGVVLEDYAMVLR
                     GSVIGPRTKIGTYTKVGRDVRIGADCSFTSFCEIRDRCDLGDRVLMGSRGTLSAGTVV
                     EDDVVMKYAFVVTDTPDLTRNDCKSVGRLKRKSRFGASVVIMPSVTIGENAEIGACSQ
                     VRKDVPDNQVWFGTPAKYYRDVALPEAAPATPEGIGAAQEPAAAH"
     gene            complement(2362..3315)
                     /locus_tag="VF453_05085"
     CDS             complement(2362..3315)
                     /locus_tag="VF453_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX7687054.1"
                     /translation="MGATMKNAGFSLPDILAALAALGVECVPRPARDCGPLRLCSYFA
                     QEPRGLYFWEGEAEPAFQHDDIVVMTARPLAGDYACQLLVAHPQRAFYLLMRHLFAPP
                     GPTRGEVHPTAVVSPAARIGPGTVIGPYCVVGDCEIGSASHLDSHVVVKDGSVIGDRV
                     TIESHSTIGATGVAWVWDPESGERIVQPQIGGARVGDDCFIGTDVTVVRGSINEVTSL
                     GAGCVVAHGTKIGHGCVVGTHVHFANNVAIAGNCRIGDRAFLSAASVLRPRVSIARGV
                     VVGAGAVVTRDVEQEDLVVSGVPARAMPSKASHSGVPRLAH"
     gene            3525..4715
                     /locus_tag="VF453_05090"
     CDS             3525..4715
                     /locus_tag="VF453_05090"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7687055.1"
                     /translation="MAAQSPIYVTQPELPPLEEFLPYLQKIWDGRQLTNGGPFHVALE
                     AALCEYLGVRHVSLFANATIALVTALQSLRVTGEVITTPYSFVATAHSAMWNGIKPVF
                     VDVDPVTLNLDPAKIEAAITPQTTAILPVHCYGTPCDVDAIQRIADDYNLKVIYDAAH
                     AFGVRREEGGGMRSILDHGDLSVLSFHATKVFNTFEGGAIVSPDAKAKQRIDHLKNFG
                     FVNETTVVATGINGKMSEFNAALGLLQLKHVGAAIERRGAIERRYRAQLQGAPGVRFL
                     DRPAGVTANHSYLPILVGPEHSAGRDGLYHALRAEGVMARRYFHPLISDFPMYRGLPS
                     ADPRGLPVARRASEQVICLPIYPALADADVDRIAGIVRDTATRARADAAPAAIAEPLL
                     LHAA"
BASE COUNT          746 a         1697 c         1719 g          744 t
ORIGIN      
        1 agagggcacc ggtgcgcatg agcccggtca tcgcgatcca ggccgccgcc caggccagca
       61 gcgcgggcag cggccagcgc aacgcaggca ggcgcgcgcg cggcggcgat gggggggcgg
      121 cggacgaggc ggacggacgc atgtcgccag tgtctcgtcg ggcgcgtccg gccgcccggt
      181 ggaaaagagc cccgaaaacc ggtcgttccc caccggcgag cccggtcggc aaagaaaaag
      241 gcgatccggt cagggatcgc cttggaaagc acaggggttg tgctaggagg agacaagcaa
      301 gaaatacact gaactagacc tgttatcggc gccggcgttc cgccgtcgag ggcgatttac
      361 aggccggtta cgtaacaaat cgagaattcg ttcacggaat tcgccgccgg tcgcccggcc
      421 gtgcgatgcg cgccacgcga ccgtggcgct ccggctcgtc cgtgggcgag ccggggtcgt
      481 cgacaccttt tctcagtggg ccagggacag cgcgcccgcc gccttgccct tgccggcacg
      541 tgccggcggg ttgcacagcc cgcacacgaa gtggcgcgcc atctcgtgcg ggtgggtgac
      601 gaaatggccg ccgcacttgg tgcacttggt catcgtcagc atgccgttgt ccacgaactt
      661 cacgaggcgc cacgcacgcg tgatcgacag cagcggctcc aggccctgcg cctcggtctg
      721 ctcctggtag agctggtagg ccttgatcac cgtgtcgatc tcctccatcg ccgcggcctt
      781 gttcaggtac tcgtggatgt tgaggaagag gctggcgtgg atgttgggct gccaggtcat
      841 gaaccagtcg gtcgagaacg ggagctgtcc cttggacggg ctcttgcccg agacttcctt
      901 gtacaggcgc agcaggcgct cgtaggacag gtcggtctcc gactccagca cctgcagtcg
      961 agcaccgagc ttgatcagcg tgaccgcacg ttcgatctgc ttggcttcgg tgaggatgct
     1021 cttgacgcgc atggcggttc tcctgcctgg ttcgttatgg tcgagggcgc ggccttcgcc
     1081 ggcggcttac gcggcttcct ggtggcggcc ggccatcagg atggaggcgt gcaggcgcga
     1141 gaccgtctcg ttggcggcgc tcttgccgtg gctggtgatc aggttccaga tcaggtcgtc
     1201 gtccatgcgg aagcggcaga gcagcgtgtt gcccgacgcg atcttcatca tctgggccgg
     1261 cgtgagggcc tggatcatgt tggccgtctc ctccgagatc cccaggcgga acagcgcctg
     1321 ttcgcgatcc gaacggatca ggctctgggc cagcatcatg taggacaggt tggcttcgcg
     1381 gatctcggcg aggatctggt tggtttccat ggtgctgttc cttgcttgtc tggtagaggt
     1441 gagaaacgtg tcaaacggtg tcaacgtggg atgaacttta aaaccgggaa ctggccggca
     1501 acataagtcc acttcggcca cttgagtcgg gcttgcgcca gcaggtctgt caggcgaatt
     1561 cctacacgtc tgtcatccgg cccaggcaaa aaatacatcg ctggggcgtt ggcccgcgcc
     1621 gcgtcgcgcg agtcccttgg gcgggaatgg gctatggaac gtccttccca tggcaaaacg
     1681 gcggccaggg cccgggcccc gaccgccgag cggcggcgat gcggcggtcg ctcagtgcgc
     1741 ggccgcgggc tcctgggcgg cgccgatccc ctcgggcgtc gcgggcgcgg cctccggcag
     1801 cgcgacgtcg cggtagtact tcgccggggt gccgaaccag acctggttgt cgggcacgtc
     1861 cttgcgcacc tgcgagcagg cgccgatctc ggcgttctcg ccgatcgtga cggagggcat
     1921 gatcaccacg ctcgcgccga agcgcgactt gcgcttcagt cgcccgacgc tcttgcagtc
     1981 gttgcgcgtc aggtcgggcg tgtcggtgac gacgaaggcg tacttcatga cgacgtcgtc
     2041 ctccacgacc gtgcccgccg agagcgtccc gcgactgccc atcagcacgc ggtcgccgag
     2101 gtcgcagcgg tcgcggatct cgcagaacga cgtgaacgag cagtccgcgc cgatgcgcac
     2161 gtcgcggccg accttggtgt acgtgccgat cttcgtgcgc gggccgatga ccgagccgcg
     2221 cagcaccatg gcgtagtcct cgaggacgac gccgtcggcc aggacgacgt cctcctcgac
     2281 gatgcagaag cgcccgatgc ggacgttcgc gccgagcgtg gcgccggggg agatgcacga
     2341 cagggtcatg gtgaaccttt ctcagtgggc gagcctgggg acgcccgagt gcgaggcctt
     2401 ggaaggcatg gcgcgggcgg ggacgcccga gaccacgagg tcctcctgct cgacatcgcg
     2461 tgtgacgacg gcgccagccc cgacgacgac accgcgcgcg atggacacgc gcggcctgag
     2521 cacgctggcg gcgctcagga aggcgcgatc cccgatccgg caattgccgg cgatcgcgac
     2581 gttgttggcg aaatggacgt gcgtcccgac cacgcagccg tggccgatct tggtgccgtg
     2641 cgcgaccacg cagccggcgc ccagcgacgt cacctcgttg atggagccgc gcacgacggt
     2701 gacgtcggtg ccgatgaagc agtcgtcgcc gacgcgcgcg ccgccgatct gcggctggac
     2761 gatgcgctcg cccgactccg gatcccagac ccaggcgacg ccggtggcgc cgatggtcga
     2821 gtgcgactcg atggtcacgc ggtcgccgat cacgctgccg tccttcacga cgacatgcga
     2881 atcgaggtgg ctggcggagc cgatctcgca gtcgccgacg acgcagtacg gcccgatcac
     2941 ggtgccgggg ccgatgcgcg ccgcgggcga gacgaccgcg gtgggatgca cctcgccccg
     3001 ggtcggcccg ggcggggcga agaggtggcg catgagcagg tagaacgcgc gctgcggatg
     3061 cgcgacgagc agctggcagg cgtagtcgcc ggcgagcggc cgggccgtca tgacgacgat
     3121 gtcgtcgtgc tggaacgcgg gctcggcctc gccttcccag aagtacaggc cgcgcggctc
     3181 ctgggcgaag tagctgcaca gccgcagcgg cccgcagtcg cgcgccgggc gcggcacgca
     3241 ctcgacgccc agcgccgcga gggcggcgag gatgtcgggc agcgagaagc cggcgttctt
     3301 catggtggcg cccatgatgg gcgctggcgc gcgcggccga tcggcggatc tgcggggcaa
     3361 aagcgcggca atccgcgccc gggccgtcgc cgcgcgcgcc ggccggcaat tggcgggcgg
     3421 atccggccca tttcccgcca tcgccgcgga gcccgggcgc ggatcatcgg cctacgcccg
     3481 ttcccgcgcg gccgcggggc ggcgcgacac caggaaagtc cgccatggca gcccaatcgc
     3541 cgatctacgt cacccagccc gagcttccgc cgctggagga gttcctgccc tacctgcaga
     3601 agatctggga cggccgccag ctcaccaacg gcggcccctt ccacgtcgcg ctcgaggcgg
     3661 cgctgtgcga gtacctcggc gtgcgccacg tctcgctgtt cgccaacgcg accatcgcgc
     3721 tcgtcacggc gctgcagtcg ctgcgcgtga cgggcgaggt gatcacgacg ccgtactcgt
     3781 tcgtcgcgac cgcccactcg gccatgtgga acggcatcaa gcccgtgttc gtcgacgtcg
     3841 atccggtgac gctcaacctc gacccggcca agatcgaggc cgcgatcacg ccgcagacca
     3901 cggccatcct gccggtgcac tgctacggca cgccgtgcga cgtcgacgcg atccagcgca
     3961 tcgccgacga ctacaacctc aaggtcatct acgacgccgc gcacgcgttc ggcgtccggc
     4021 gcgaggaggg cggcgggatg cgctcgatcc tcgaccacgg cgacctgtcg gtgctcagct
     4081 tccacgcgac caaggtcttc aacaccttcg agggcggcgc gatcgtctcg cccgacgcga
     4141 aggccaagca gcgcatcgac cacctgaaga acttcggctt cgtcaacgag acgacggtcg
     4201 tggccacggg catcaacggc aagatgagcg agttcaacgc cgcgctgggc ctgctgcagc
     4261 tcaagcacgt cggcgcggcc atcgagcgcc gcggcgccat cgaacgccgc taccgggcgc
     4321 agctgcaggg cgcgccgggg gtgcgcttcc tcgaccgccc ggccggcgtc accgccaacc
     4381 actcgtacct gccgatcctc gtcggccccg agcattcggc cgggcgggac ggcctctacc
     4441 acgcgctgcg cgccgagggc gtgatggcgc ggcgctactt ccatccgctg atctccgact
     4501 tcccgatgta ccgcggtctg ccgtcggccg atccgcgcgg cctgccggtc gcgcgccgcg
     4561 cgtcggagca ggtgatctgc ctgccgatct accccgccct cgcggacgcg gacgtcgacc
     4621 gcatcgccgg catcgtccgc gacaccgcca cgcgcgcccg cgccgacgcg gcgcccgcgg
     4681 cgatcgcgga gcccttgctg ctgcacgccg cctgagccgc ccgcaggcgc ttgcaccgca
     4741 gtgcggagca cggttgccca tgagcctcgc gccgatgccc ggcgtgcgcc aaatggccgg
     4801 ccttggcccg attcatcccc cgatcgcgcg cgaccccgga cgcgcatcat ggggcatgac
     4861 ctccgagatc cgccccaagc ccgcccgcag cacgctggtc gtcgtc
//
LOCUS       DASVLN010000225        13173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_386631, whole genome shotgun sequence.
ACCESSION   DASVLN010000225 DASVLN010000000
VERSION     DASVLN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13173
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386631"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VF453_05095"
     CDS             <1..1030
                     /locus_tag="VF453_05095"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7687056.1"
                     /translation="EPYAPATAAAVVRPPVAGQPLPPPSRTLQEEMRQASAVCNRGRE
                     RVEKMFSEARLGRVIDAEACQPLVQEISDSVIRNPGALVSLARLKTQDDYSYMHSVAV
                     CALMVALGRELGFDEEQCRRAGTAGLLHDVGKALMPLEILNKPGKLTDEEFDVMRSHP
                     LRGHELLLEARGADPAAMDVCLHHHEKMDGSGYPYKLTPDRLTQLARMGAVCDVYDAI
                     TSNRPYKAGWDPAESIGKMASWKGHFDPQIFAAFVKSVGIYPTGSLVRLHSERLAVVL
                     EQNPQALTAPVVRVFFSLKSNMPVSLQRLDLSSGTSDRIVGRESPERWNFAFLDELWA
                     GDAAPKRR"
     gene            complement(1137..2810)
                     /locus_tag="VF453_05100"
     CDS             complement(1137..2810)
                     /locus_tag="VF453_05100"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX7687057.1"
                     /translation="MRSTTLRLASLLLLTPIARLALAQVQPGAGDLLQQVHPPVVPST
                     AQPAPSIRLDAARAKDDLSAVKFELRHIRITGNTVVDESTLHALVQDLEGRTVTLGEV
                     SEAVARITRYYQRQGYPFSRALIPAQSIRQGELTVQVLEARYGDVRLSGNKSPVASAL
                     MREILAPLKPGAPVADASLDRALLLLSDLPEVSVGATASPGSEPGSTDLDVQAQELPT
                     PHFDFGLDNFGSRYTGRARVSAAGSLFNPLHRGDTLGLALLTSGSGMRYARASYDIVL
                     GGSGTHAGVAYSAMRYHLGEDASALDAHGSAGVSSAWLRYPVLRGRRANVYVTAEFDH
                     KRLRDDIGATGIDNQRHVDSGVLSLSGDLQSWPGDGASGWTLALATGHVGFDNSAAGA
                     ADQASARTRGSFTKWNLDASHQQRLTADGALYLAVSVQATDANLDSAEKLSVGGPYSV
                     RAYESGALSGDSGGTATVEWRQGLGALSGGRLQLRGFFDAAHLRINHRTWAAVDNGAT
                     IRGAGAGLSWSNASLWTVDAVLASRIGAAPAQAGRTASTRGWIMVSRAI"
     gene            complement(2845..8487)
                     /locus_tag="VF453_05105"
     CDS             complement(2845..8487)
                     /locus_tag="VF453_05105"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YDG domain-containing protein"
                     /protein_id="HEX7687058.1"
                     /translation="MHGHASQNKIFRLVWNAARRAWVAVAETARGRGGKTRRRAAAST
                     LLLGAAHALAGPQGGTVTSGSATIRQAGSVTTITQSSSNVALDWQSFDTTAQESVRFV
                     QPSATAVAVNRISDTSATRFFGRLDANGQVWLINPNGVLFGASSQVDVGGLVASTLDL
                     APGGGAHFEGAGTGSIVNQGHISAAEGGYVALVGNTVGNAGSISAPLGSVALAAGSDV
                     SLTFAQDRLLSVTVKRSTLNNLAENGGLIAADGGTVLLSAGARDAVLASAVNNSGIVE
                     ARSVHAVDGTIVLDGGGSGSASNSGTLDASGRAAGETGGTVHVLSGNAVNLAAGSLVD
                     VSGDAGGGTALVGGNFQGGGSEQHALAATVAAGAGIRADALTTGQGGTAVVWSDGSTQ
                     FDGGISARGGTQAGNGGQVETSGHRLKVSASAAVDTSAPGGTAGNWLLDPDDVVIGNA
                     SLWGSSFHIDVDSVALTNALNSGSVTVKTTASHGSCSGVACTTGTAGNGDIVILDFIG
                     AVSDYNNGGFGYNWTSGSTLTLSAYRDIHFVTTTGVPNATDPTGSSTTWGGGIEIDGP
                     GKAVLHADNAARGTGTVRFDDNFNSFLYAGNSSSAIDIYYSPTSFSSPTDYSIYDAGG
                     AGTITSYMAVNVGVTIANKTYDGTTSAVIHSAPTVASLPSGVTVSTAGATAQFSDKNA
                     GTGKSVSLLGVTFGGGGTTTTDGSGQHYYLNGLASATADIAKADLAVSGVSASNKTYD
                     ATTTATLTGTASVTALGGDVVSVSGTGIGTFADKNAGSNKAVTVTGYTLAGTDAGNYN
                     VIQPTGLTATISKADLAVSGVSANNKTYDTTTTATLTGTASVTALGSDVVSVTGTGIG
                     TFVDKNAGSNKSVTVTGYTLAGTDAGNYNVIQPAGLTATIAKADLAVSGVTASDKTYD
                     ATVAATLSGTASVTALGSDVVSVSGTGVGTFVDKNAGSNKAVTVTGYTLAGTDAGNYN
                     VIQPTGLTATIAKADLAVSGVTASNKTYDTTTSATLTGTASVTALGSDVVSVSGTGIG
                     TFADKNAGSNKAVTVTGYTLTGTDAGNYNVIQPAGLTATIDKADLAVSGVTASNKTYD
                     ATTAATLSGAASVTALGSDVVSVSGTGIGTFVDKNAGSNKSVAVTGYTLAGTDAVNYN
                     IVQPTGLTATIDKADLAVSGITAGNKTYDATTAATLSGTASVTALGSDIVSVSGTGLG
                     AFSDKNAGSNKSVTVTGYTLAGTDAGNYSVIQPTGLTATISKADLAVSGVTASNKTYD
                     TTTSATLTGTASVTALGTDVVSVTGTGIGTFVDKNAGSNKSVTVTGYTLAGTDAVNYN
                     IVQPTGLTATISKADLAVSGVSASNKTYDATTTATLTGTASVTALGGDVVSLTGTGIG
                     TFVDKNAGSNKSVTVTGYTLAGTDAGNYNVIQPTGLTATIAKADLAVSGVTASNKTYD
                     TTTSATLIGTASVTALGSDVVSVSGTGIGTFADKNAGSNKAVTVAGYTLTGTDAGNYN
                     VIQPAGLTATISKADLAVSGVTASNKTYDATTAATLSGTASVTALGSDMVSVTGTGIG
                     TFADKNAGSNKSVTVTGYTLAGTDAANYNVIQPTGLTATIAKADLAVSGITASNKTYD
                     ATTNATLAGTASVTALGSDVVSVSGTGIGTFADKNAGSNKSVTVTGYTLAGTDAGNYT
                     IEQPTGLTASIAQAPLVVSGITAADKVFDGSTAAVVSTANAELQGLQAGDQVTLSATG
                     AFTDAAIGAGKTVTLSSSYGGADVGNYAIVGQASASASILAASAGTPPPPTVSPTQQA
                     VLANVVPMIQSGGLSLASATATLPSDAGVDAPSVVAIPTAPPALSVSASSQQAPRPLE
                     LLAPQFESPPIVVSSPNARLTLQIRRGGVRLPSAPNAE"
     gene            complement(8719..11814)
                     /locus_tag="VF453_05110"
     CDS             complement(8719..11814)
                     /locus_tag="VF453_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7687059.1"
                     /translation="MKFTDLFIKRPVLAGVISLLIVVMGLRSLFSLPVAQYPRTQNSV
                     VTISTTYYGADAQTVAGFITQPLEGAIAQAQGIDYLSSSSSSGVSTITATLRLNYDAN
                     RALTEINTQVNSVKNQLPAQAQQPVLTVATGQTTDAMYMGFYSDTLPTNNVTDFLLRV
                     VKPKLDSVPGVQTAELLGARQFALRAWLDADKLAAHGLSAADVSQALAANNYLAALGS
                     SKGQMVTVPLTAGTDLHSLDEFKHLIVKQSGDSVVRLEDVATVTLGSENYDFNVAFGG
                     VRSVFIGIKVAPEANILDVAKSVREAFPDLKAQLPSGVTGEIVYDSTDFINTSIKEVV
                     KTLVEALLIVTVVIYLFLGTFRAVVVPLVAMPLSLIGAFFIMLALGYSINLLTLLALV
                     LAIGLVVDDAIIVVENVDRHMKEEHKTPLQAALVAARELAGPIMAMTVVLVAVYVPIG
                     FQGGLTGALFTEFAFTLAAAVFVSGIVALTLSPMMCSRIFRADQDDGKFAHAIDHVFE
                     KVRGRYQRILGGLLKTYPVVIVMGLLLFALLGVMFMMSQGELAPEEDQGIVLSQVVGA
                     PNATSDQMQTYAKQVFDIAHQAPEYKQMFQITGVPTVNAGIGGVLLKDWGDRTRSAHE
                     IQVDLQAKWNRIAGARVAAFQFPPLPGTSGLPVQFVITTTEPFENLNTVAQQVMDKAR
                     ASGKFYFLDQDLKLDAPQAKVVVDRDKIAALGMTQQDVGSALTAALGGGYVNYFSIAG
                     RSYKVIPQVLQVDRLNPSNVLDFYIKTPSAGLIPASTIAHLEYSVQPEAITRFQQLNS
                     VTISGVAGGSQGDTLKFLQDTLKQVAPSGYNADYAGQSRLFHNESGGFVITLAFAVII
                     VFLALAAQFESFRDPVVILMSVPQALFGAMIFIFWGFSSVNIYTQVGLVTLMGLISKH
                     GILIVEVANNEQKRGLSKMEAILSATSIRLRPILMTTAAMVFGVVPLVIASGAGAAGR
                     QAMGLVIFTGMSIGTLFTLFVVPAFYLLLGADHHKRSQASEDADAAIDEVDHEQPKPA
                     H"
     gene            complement(11831..12988)
                     /locus_tag="VF453_05115"
     CDS             complement(11831..12988)
                     /locus_tag="VF453_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7687060.1"
                     /translation="MTKRMLIMIGCVLLLVLVLAGGFALHIKKLMSSFPKPTPSVVTA
                     ARVEPLEWQPQLSAVGSVTPVRGVDVTTEIAGQVREILFKSGQDVRKGDVLVELNADT
                     DKAQLQSLQAAADLAATVLKRDQGQFAAQAIAQAQVDADQADLKSKRALVAQQQAVID
                     KKTIRAPFSGKLGITTVNPGQYLNPGDKIVTLQTIDPVYVDFNLPQKEVGNLSTGQDV
                     IVQTDGFPGVHFPGKITAISPKVDTTTRNIVVEATIPNAKRQLLPGMFANVLVDVGEK
                     KRYLTLPQTAITYNPYGSTVFVVVDAAEATRAAKAASAPAPAASEGLVVQQAFVTTGE
                     TRGDQVAILKGLKEGQQVVTSGQVKLKNGAPVKIDNSIVPANSPNPTPQEH"
BASE COUNT         1816 a         4348 c         4815 g         2194 t
ORIGIN      
        1 cgagccgtat gcgccggcca ccgccgcggc cgtggtgcgg ccgcccgtcg ccggccagcc
       61 gctgccgccg ccgtcccgga ccctgcagga ggaaatgcgc caggcctcgg ccgtctgcaa
      121 ccgcggccgc gaacgggtcg agaagatgtt cagcgaggcg cgcctcggcc gagtcatcga
      181 cgccgaggcc tgccagccgc tggtgcagga gatctcggac tccgtcatcc gcaacccggg
      241 cgcgctggtg agcctcgcgc gcctgaagac gcaggacgac tactcctaca tgcactcggt
      301 ggccgtgtgc gcgctgatgg tggcgctcgg tcgcgagctc ggcttcgacg aggagcagtg
      361 ccgccgcgcc ggcaccgccg gcctgctgca cgacgtcggc aaggcgctga tgccgctgga
      421 gatcctcaac aagcccggca agctcaccga cgaggagttc gacgtcatgc gcagccaccc
      481 gctgcgcggc cacgagcttc tgctggaggc gcgcggtgcc gatcccgcgg ccatggacgt
      541 ctgcctgcac caccacgaga agatggacgg ctccggctac ccgtacaagc tgacgcccga
      601 tcgcctgacc cagctcgcgc gcatgggcgc ggtctgcgac gtctacgacg cgatcacctc
      661 gaaccgtccc tacaaggcgg gctgggatcc ggccgaatcg atcggcaaga tggcgagctg
      721 gaagggccac ttcgacccgc agatcttcgc ggccttcgtc aagagcgtgg gcatctaccc
      781 gaccggctcg ctcgtgcgcc tgcattccga gcgcctggcc gtcgtcctcg agcagaaccc
      841 gcaggcgctg accgcgccgg tcgtgcgcgt cttcttctcg ctgaagtcca acatgcctgt
      901 ctcgctgcag cgcctcgacc tctcgagcgg caccagcgac cgcatcgtcg gccgcgagtc
      961 gccggaacgc tggaacttcg ctttcctcga cgagctgtgg gcgggagatg cggcgccgaa
     1021 gaggcgctga gcggcgctga gcggttgcgc gagggccggg atgacttgcc gcgtcgcgcg
     1081 aattctcgca cccggaagcc accgccccgg atcggcgagc cgggcgcgcc ggcgggtcag
     1141 atcgcccggc tgaccatgat ccagccgcgt gtggacgccg tgcggccggc ctgggccgga
     1201 gcggcgccga ttcgcgatgc cagcacggcg tcgacggtcc agagggacgc gttgctccag
     1261 ctcaggcccg cgccggcgcc gcggatcgtg gcgccgttgt cgacggctgc ccacgtccgg
     1321 tggttgatgc gcaggtgggc ggcgtcgaag aagccccgca gctggaggcg cccgccggac
     1381 agggcgccca gtccctggcg ccattcgacc gtggccgtgc cgccggaatc gccggagagc
     1441 gcgccgctct cgtaggcccg caccgaatag gggccgccca ccgacagctt ctccgccgag
     1501 tcgagattcg catccgtcgc ctgcaccgac acggccaggt agagcgcgcc gtcggccgtc
     1561 aggcgctgct ggtgcgatgc gtcgaggttc cacttcgtga acgagccgcg ggtccgggcc
     1621 gacgcctggt cggcggcgcc ggcggccgaa ttgtcgaagc cgacgtggcc ggtggccagg
     1681 gccagggtcc acccgctcgc gccatcgccc ggccacgact gcagatcgcc gctcaggctg
     1741 aggacgccgc tgtcgacgtg gcgctggttg tcgatgccgg tcgcgccgat gtcgtcgcgc
     1801 aggcgcttgt ggtcgaactc ggccgtcaca tagacgttcg cgcgcctgcc gcgcagcacg
     1861 ggataccgca gccaggcgct gctcacgccg gccgagccgt gcgcatcgag cgcgctggcg
     1921 tcttcgccca ggtggtagcg catcgcggaa tacgcgacgc cggcatgggt gcccgagccg
     1981 ccgagcacga tgtcgtagga agcgcgcgcg tagcgcatgc cgctgccgct ggtgagcagc
     2041 gcgaggccga gcgtgtcgcc gcgatgcagc gggttgaaga ggctgccggc ggcgctgacg
     2101 cgcgcgcgac cggtgtagcg gctgccgaag ttgtccaggc cgaagtcgaa gtgcggcgtg
     2161 gggagttcct gcgcctggac gtccaggtcg gtcgagccgg gctcgctccc cggcgaagcc
     2221 gtggcgccca cggagacttc cggcaggtcg gacagcagca gcagggcccg atccagggac
     2281 gcatccgcca cgggtgcgcc aggcttcagg ggggccagga tctcgcgcat cagggcgctc
     2341 gcgacgggac tcttgttgcc gctcaggcgg acgtcgccgt agcgggcttc gagaacctgg
     2401 accgtcagtt cgccctgccg gatcgactgg gccgggatca gcgcacgcga gaacggatag
     2461 ccctggcgct ggtagtagcg cgtgatgcgg gccacggcct cgctgacttc cccgagcgtc
     2521 acggtgcggc cttccaggtc ctgcacgagc gcatgcaagg tcgactcgtc gacgaccgtg
     2581 ttgccggtga tgcggatgtg gcgcagctcg aacttgaccg cggacaggtc gtccttcgcc
     2641 cgggcggcgt cgagccggat cgacggcgcg ggctgcgcgg tcgacgggac gacgggcgga
     2701 tgcacctgct gcaggagatc gccggcaccg ggctggacct gggccagcgc caggcgcgcg
     2761 atcggggtca gcagcagcag cgaggcgagg cgaagagtgg tcgaacgcat ggtttgtcgt
     2821 gtggtcgatg cggggcgccg agggtcactc ggcgttgggg gcggacggca ggcggacgcc
     2881 gccgcgccgg atctgcagcg tcaagcgcgc gttggggctg gagacgacga tcggcggcga
     2941 ttcgaattgc ggcgccagca gttccagggg ccggggcgct tgctggctcg acgcgctgac
     3001 ggacagggcg ggcggcgccg tcggaatggc gacgaccgaa ggcgcatcca cgccggcgtc
     3061 gctcgggagg gtggccgtcg ccgaggccag ggaaaggccg ccggactgga tcatcggcac
     3121 gacattggcc agcacggcct gctgggtcgg cgacaccgtc ggcggtgggg gggtgcccgc
     3181 gctcgcggcg aggatgctcg cgcttgcgct ggcctggccc acgatggcgt agttgcccac
     3241 gtccgcgccg ccgtacgaac tgctgagggt gaccgtcttg cccgcgccga tcgccgcgtc
     3301 ggtgaaggcc cccgtggcgc tgagcgtgac ctggtcgcct gcctgcaagc cctgcagctc
     3361 cgcgttcgcg gtcgacacga cggccgcggt cgacccgtcg aacaccttgt cggcagccgt
     3421 gatgcccgat acgacgagcg gggcctgggc gatcgaggcc gtgagcccgg tgggctgctc
     3481 gatcgtgtag ttgccggcgt cggtgccggc cagcgtgtat cccgtgacgg tcacggactt
     3541 gttcgagccg gcgttcttgt cggcgaaggt gccgatgccg gtgcccgaga ccgagaccac
     3601 gtcgctgccc agcgccgtca ccgaggcggt gccggcgagc gtcgcgttgg tcgtggcgtc
     3661 gtaggtcttg ttgctggccg tgatgccgct gaccgcgagg tcggccttgg cgatggtggc
     3721 cgtcaggccg gtgggctgga tgacgttgta gttggcggcg tcggtgccgg ccagggtata
     3781 gccagtgacc gtcaccgact tgttcgagcc ggcgttcttg tcggcgaacg tgccgatgcc
     3841 ggtgcccgtc accgagacca tgtcgctgcc cagcgccgtc acggaagccg tgcccgacaa
     3901 ggttgcggcc gtggtggcgt cgtaggtctt gttgctggcg gtgacgccgc tgaccgcgag
     3961 atcggccttg gagatggtgg cggtcaggcc ggcgggttgg atgacgttgt agttgccggc
     4021 gtcggtgccg gtcagcgtgt agccagcgac ggtcacggcc ttgttcgaac cggcgttctt
     4081 gtcggcgaag gtgccgatgc cggtgcccga caccgagacc acgtcgctgc ccagtgccgt
     4141 caccgaggca gtgccgatga gcgtcgcgct ggtcgtggtg tcgtaggtct tgttgctggc
     4201 ggtgacgccg ctgaccgcga ggtcggcctt ggcgatggtg gccgtcagtc cggtgggctg
     4261 gatgacgttg tagttgccgg cgtcggtgcc ggccaacgtg tagccggtga cggtcacgga
     4321 cttgttcgag cccgcgttct tgtcgacgaa cgtgccgatg ccggtgcccg tcagcgagac
     4381 cacgtcgccg cccagggccg tcaccgaggc ggtgccggtg agcgtcgcgg tggtggtggc
     4441 gtcgtaggtc ttgttgctgg cgctcacgcc gctgaccgcg aggtcggcct tggagatggt
     4501 ggcggtcagg ccggtgggct ggacgatgtt gtagttgacg gcgtcggtgc cagccagggt
     4561 atagccggtg accgtcaccg acttgttcga gccggcgttc ttgtcgacga acgtgccgat
     4621 gccggtgccc gtcaccgaga cgacgtcggt gcccagggcc gtcaccgagg cggtgccggt
     4681 gagggtcgcg ctggtcgtgg tgtcgtaggt cttgttgctg gcggtgacgc cgctgaccgc
     4741 gaggtcggcc ttggagatgg tggcggtcag gccggtgggc tggatgacgc tgtagttgcc
     4801 ggcgtcggtg ccggccagcg tgtagccggt gacggtcacg gacttgttcg agcccgcgtt
     4861 cttgtcggag aaggcgccga ggccggtgcc gctcaccgag acgatgtcgc tgcccagtgc
     4921 cgtcacggag gccgtgcccg acaaggtcgc ggccgtggtg gcgtcgtagg tcttgttgcc
     4981 ggccgtgatg ccgctgaccg cgaggtcggc cttgtcgatg gtggcggtca ggccggtggg
     5041 ctggacgatg ttgtagttga cggcgtcggt gcctgccaac gtgtagccgg tgacggccac
     5101 ggacttgttc gagcccgcgt tcttgtcgac gaacgtgccg atgccggtgc cgctgacgga
     5161 gaccacgtcg ctgcccagcg ccgtcacgga ggccgcgccc gacaaggttg cggccgtggt
     5221 ggcgtcgtag gtcttgttgc tggcggtgac gccgctgacc gcgaggtcgg ccttgtcgat
     5281 ggtggcggtc aggccggcgg gttggatgac gttgtagttg ccggcgtcgg taccggtcag
     5341 cgtgtagccg gtgacggtca cggccttgtt cgaaccagcg ttcttgtcgg cgaaggtgcc
     5401 gatgccggtg cccgacaccg agaccacgtc gctgcccagt gccgtcaccg aggcagtgcc
     5461 ggtgagcgtc gcgctggtcg tggtgtcgta ggtcttgttg ctggcggtga cgccgctgac
     5521 ggcgaggtcg gccttggcga tggtggccgt cagtccggtg ggctggatga cgttgtagtt
     5581 gccggcgtcc gtgccggcca acgtgtagcc cgtgacggtt accgccttgt tcgagccggc
     5641 gttcttgtcg acgaaggtgc cgacgccggt gccgctcacg gagaccacgt cgctgcccag
     5701 tgccgtcacg gaggccgtgc ccgacaaggt tgcggccacg gtggcgtcgt aggtcttgtc
     5761 gctggcggtg acgccgctga cggcgaggtc ggccttggcg atggtggccg tcaggccggc
     5821 gggttggatg acgttgtagt tgccggcgtc cgtgccagcc agggtatagc cagtgaccgt
     5881 caccgacttg ttcgagccgg cgttcttgtc gacgaacgtg ccgatgccgg tgcccgtcac
     5941 cgagacgacg tcgctgccca gtgccgtcac cgaggcggtg ccggtgagcg tcgcggtggt
     6001 cgtggtgtcg taggtcttgt tgttggcgct cacgccgctg accgcgagat cggccttgga
     6061 gatggtggcc gtcagtccgg tgggctggat gacgttgtag ttgccggcgt cggtgccggc
     6121 caacgtgtag cccgtgacgg ttaccgcctt gttcgagccg gcgttcttgt cggcgaaggt
     6181 gccgatgccg gtgcccgaca ccgagaccac gtcgccgccc agggccgtca ccgaggcggt
     6241 gccggtgagc gtcgcggtgg tcgtggcgtc gtaggtcttg ttgctggcgc tcacgccgct
     6301 gacggcgagg tcggccttgg cgatgtcggc ggtcgcgctg gccaggccgt tcaggtagta
     6361 gtgctggccg ctgccatccg tggtggtggt gccgccgccg ccgaaggtga cgccgagcag
     6421 gctcaccgac ttgcccgtgc cggcgttctt gtcggagaac tgcgccgtgg cgccggcggt
     6481 gctcaccgtc acgccggagg gcaggctggc caccgtgggc gccgagtgga tgacggccga
     6541 ggtcgtgccg tcataggtct tgttggcgat cgtcaccccc acgttgacgg ccatgtagga
     6601 cgtgatcgtg ccggcgccgc cggcgtcgta gatgctgtag tcggtcggtg aactgaagct
     6661 cgtcgggctg tagtagatgt cgatggcgct gctgctgttg cccgcgtaga ggaagctgtt
     6721 gaagttgtcg tcgaacctca ccgtgcccgt cccgcgcgcc gcgttgtcgg cgtgcagcac
     6781 cgccttgccg gggccatcga tctcgatgcc tcccccccag gtcgtcgacg agcccgtggg
     6841 atcggtcgcg ttcggcacgc cagtggtggt cacgaagtgg atgtcgcgat acgcgctcaa
     6901 ggtcagcgtg ctgccgctcg tccagttgta gccgaagccg ccgttgttgt agtcgctcac
     6961 cgcgccgatg aagtcgagga tgacgatgtc gccgttgccc gcggtgccgg tcgtgcaagc
     7021 gacgccgctg cagctgccgt gcgacgccgt cgtcttgacg gtgacgctgc cgctgttgag
     7081 cgcgttggtc agcgcgacgg aatcgacgtc gatgtggaat gacgatcccc acaggctggc
     7141 attgccgatg acgacgtcgt ccggatccag cagccagttg ccggccgtgc cgccgggcgc
     7201 cgaggtgtcg accgcggccg aggcgctcac cttcaaccgg tggccggacg tctcgacctg
     7261 gccgccgttg cctgcctggg tgccgccgcg cgcgctgatg ccgccgtcga actgcgtgct
     7321 gccatcggac cacacgaccg cggtgccgcc ctggcccgtc gtcagggcgt ccgcccggat
     7381 gccagcaccc gccgcgacgg tggccgccag tgcgtgctgc tcgctgccgc caccctggaa
     7441 attcccgccg accagcgccg taccgccgcc ggcatcgccg ctgacgtcga cgaggctgcc
     7501 ggcagccagg ttcaccgcat tgcccgacag gacgtgtacc gtcccgccgg tctcccccgc
     7561 cgcgcggccc gacgcgtcca gcgtgccgct gttcgacgcg ctgcccgagc cgccgccatc
     7621 gagcacgatc gttccgtcga cggcatgcac gctgcgcgcc tcgacgatgc cgctgttgtt
     7681 gaccgcgctg gccagcaccg cgtcacgcgc acccgccgac agaagcacgg tgccgccgtc
     7741 cgccgcgatc agcccgccgt tctcggccag gttgttcaag gtgctgcgct tcaccgtcac
     7801 ggaaagcagc cggtcctggg cgaaggtcag gctcacatcg ctgcccgcgg ccagcgccac
     7861 cgagcccagc ggggcactga tgctgccggc gttgcccacc gtgttcccga ccagggccac
     7921 atagccgccc tcggcagcgg agatgtgccc ctggttgacg atggagccgg tgccggcccc
     7981 ttcgaaatgc gcgccgcccc cgggcgccag gtcgagcgtg gaggccacca gcccaccgac
     8041 gtcgacctgc gagctggcgc cgaacagcac gccgttcggg ttgatcagcc agacctggcc
     8101 attggcgtcg agacggccga agaaccgggt ggccgaggtg tcggagatgc ggttgaccgc
     8161 gacggcggtg gccgagggct gcacgaaccg cacggactcc tgggcggtgg tgtcgaacga
     8221 ctgccagtcg agcgcgacgt tggagctcga ctgcgtgatc gtggtgacgc tgccggcttg
     8281 acggatggtg gcgctgccgg aggtcaccgt cccgccctgc gggcctgcca gcgcatgcgc
     8341 cgcgcccagg agcagggtcg aggcggccgc ccgccggcgc gtcttgccgc cgcggccccg
     8401 ggcggtctcg gccactgcca cccaggcccg gcgggccgca ttccagacga ggcggaagat
     8461 cttgttctga ctggcgtgac cgtgcacggg gcgctccttg gatggtctct acctcaggaa
     8521 gccggcccgg ttcggccggc tcctggatcg ttgccttcgg atgctagcca cgatgccacg
     8581 cggaccatcc gtgaagaagc gcccgcaagc gtgactacat cacggcttca cggcagtcgt
     8641 ccgggcgtgc acggagcgcc ccgccatgaa aaaggcccgg catcgctgcc gggccctcgc
     8701 cttcaggaag ccgcgcgttc agtgcgccgg cttcggctgc tcgtggtcga cctcgtcgat
     8761 cgccgcgtca gcgtcctcgg acgcctgcga gcgcttgtgg tggtcggcgc ccagcagcag
     8821 gtagaacgcc ggcacgacga acagcgtgaa cagcgtgccg atggacatgc ccgtgaagat
     8881 cacgaggccc atcgcctgcc gtccggcggc gcctgcgccc gaggcgatca ccagcggcac
     8941 cacgccgaag accatcgcgg cggtggtcat caggatgggg cgcagccgga tggacgtggc
     9001 cgagaggatg gcctccatct tcgacaggcc gcgcttctgc tcgttgttgg cgacctcgac
     9061 gatcaggatc ccgtgcttgg agatcagccc catcagcgtc accaggccga cctgggtgta
     9121 gatgttgacc gagctgaagc cccagaagat gaagatcatc gcgccgaaga gggcctgcgg
     9181 cacggacatc aggatgacga ccgggtcgcg gaagctctcg aactgcgcgg ccagcgccag
     9241 gaacacgatg atgaccgcga acgcgagcgt gatcacgaag ccgccggact cgttgtggaa
     9301 caggcgcgac tggccggcgt agtcggcgtt gtagccggag ggtgcgacct gcttgagcgt
     9361 gtcctgcagg aacttcagcg tgtcgccctg cgagccgcct gccacgcccg agatcgtgac
     9421 cgagttcagc tgctggaagc gcgtgatcgc ctccggctgc acgctgtact cgaggtgggc
     9481 gatcgtgctc gccgggatca ggccggcgct gggcgtcttg atgtagaagt ccagcacgtt
     9541 ggacgggttc agccggtcga cctggagcac ctgcgggatc accttgtacg agcggcccgc
     9601 gatcgagaag tagttgacgt agccgccgcc cagcgccgcc gtcagcgcgg agccgacgtc
     9661 ctgctgggtc atcccgagcg cggcgatctt gtcgcggtcg acgacgacct tggcctgcgg
     9721 cgcgtccagc ttcaggtcct ggtcgaggaa gtagaacttg cccgacgcgc gcgccttgtc
     9781 catcacctgc tgcgcgacgg tgttcaggtt ctcgaacggc tccgtcgtgg tgatgacgaa
     9841 ctgcaccggc aggcccgagg taccgggcag cggcgggaac tggaaggccg ccacgcgcgc
     9901 gccggcgatc ctgttccact tcgcctgcag gtcgacctgg atctcgtgcg cgctgcgggt
     9961 gcggtcgccc cagtccttga gcagcacgcc gccgatgccg gcgttgaccg tcggcacgcc
    10021 ggtgatctgg aacatctgct tgtactcggg cgcctggtgc gcgatgtcga agacctgctt
    10081 ggcgtaggtc tgcatctggt cggaggtcgc gttgggcgcg cccacgacct gcgacagcac
    10141 gatgccctgg tcctcctccg gcgccagctc gccctgcgac atcatgaaca tgacgcccag
    10201 cagcgcgaac agcagcaggc ccatcacgat gacgacgggg taggtcttga gcagtccgcc
    10261 caggatgcgc tggtagcgcc cgcgcacctt ctcgaagacg tggtcgatcg cgtgcgcgaa
    10321 cttgccgtcg tcctggtcgg cgcggaagat gcgcgagcac atcatcggcg acagcgtcag
    10381 cgcgacgatg ccggagacga acaccgccgc cgccagcgtg aacgcgaact cggtgaacag
    10441 ggcgccggtg aggccgccct ggaagccgat cggcacgtac accgcgacca gcaccaccgt
    10501 catcgccatg atgggccccg cgagctcgcg cgcggcgacc agcgcggcct gcagcggcgt
    10561 cttgtgctcc tccttcatgt ggcggtcgac gttctccacc acgatgatgg cgtcgtcgac
    10621 caccaggccg atggccagca ccagcgcgag cagcgtgagc aggttgatcg agtacccgag
    10681 cgccagcatg atgaagaagg cgccgatcag cgacaacggc atcgccacca gcggcaccac
    10741 cacggcgcgg aacgtgccga ggaacaggta gatcaccacc gtcacgatca gcagcgcctc
    10801 gaccagcgtc ttcaccactt ccttgatgga ggtgttgatg aagtcggtgg agtcgtagac
    10861 gatctcgccg gtcacgccgc tcggcagctg cgccttgagg tcggggaagg cctcgcgcac
    10921 cgacttggcg acgtccagga tgttggcctc cggcgccacc ttgatgccga tgaacaccga
    10981 gcgcacgccg ccgaacgcga cgttgaagtc gtagttctcg gagcccagcg tgacggtggc
    11041 gacatcctcc agccgcacga cggagtcgcc gctctgcttg acgatcaggt gcttgaactc
    11101 gtcgagcgag tgcaggtcgg tgccggccgt cagcggcacg gtgaccatct ggcccttgct
    11161 cgagccgagc gcggcgaggt agttgttggc cgccagcgcc tggctcacgt cggccgcgga
    11221 caggccgtgg gccgcgagct tgtcggcatc cagccaggcg cgcagcgcga actggcgtgc
    11281 gcccagcagc tcggcggtct gcacgccggg cacggagtcg agcttgggct tgacgacgcg
    11341 cagcaggaag tcggtgacgt tgttcgtggg cagcgtgtcg ctgtagaagc ccatgtacat
    11401 cgcgtcggtc gtctgccccg tcgcgaccgt cagcaccggc tgctgcgcct gcgcgggcag
    11461 ctggttcttg accgagttga cctgcgtgtt gatctcggtc agcgcgcggt tggcatcgta
    11521 gttcaggcgc agcgtggccg tgatcgtgga cacgcccgag gagctcgacg acgagaggta
    11581 gtcgatgccc tgggcctgcg cgatcgcgcc ttcgagcggc tgcgtgatga agcccgcgac
    11641 ggtctgggcg tcggcgccgt agtaggtcgt cgagatcgtg acgaccgagt tctgcgtgcg
    11701 cgggtactgc gcgaccggca ggctgaacag cgatcgcagc cccatcacga cgatgagcag
    11761 gctgatcacg ccggccagca ccggccgctt gatgaagaga tcggtgaact tcatggcgaa
    11821 agccttccgc tcagtgttcc tgcggggtgg ggttcgggct gttggcaggc acgatcgagt
    11881 tgtcgatctt caccggcgcg ccgttcttca gcttgacctg gccgctggtg acgacctgct
    11941 ggccctcctt caggcccttc aggatcgcga cctggtcgcc gcgggtctcg cccgtcgtca
    12001 cgaaggcctg ctgcacgacg aggccctccg aggcggccgg cgcgggcgcc gacgcggcct
    12061 tcgcggcgcg cgtcgcctcg gcggcgtcga cgaccacgaa caccgtcgag ccgtacgggt
    12121 tgtacgtgat cgcggtctgc ggcagcgtca ggtagcgctt cttctcgccg acgtcgacca
    12181 gcacgttggc gaacatgccg ggcagcagct ggcgcttcgc gttggggatc gtggcctcga
    12241 cgacgatgtt gcgcgtggtc gtgtcgacct tcgggctgat cgccgtgatc ttgccgggga
    12301 agtgcacccc cgggaagccg tcggtctgca cgatcacgtc ctggccggtg gagaggttgc
    12361 cgacttcctt ctgcggcagg ttgaagtcga cgtagacggg atcgatggtc tgcagcgtca
    12421 cgatcttgtc gcccgggttc aggtactggc cggggttgac cgtcgtgatg cccagcttgc
    12481 ccgagaaggg cgcccggatg gtcttcttgt cgatgaccgc ctgctgctgc gcgacgagcg
    12541 cgcgcttgct cttcaggtcg gcctggtcgg cgtcgacctg cgcctgcgcg atcgcctgcg
    12601 cggcgaactg gccctggtcg cgcttgagca ccgtggccgc gaggtcggcg gcggcctgca
    12661 gggactgcag ctgcgccttg tccgtgtcgg cgttgagctc gaccagcacg tcgcctttcc
    12721 tcacgtcctg gccggacttg aagaggatct cgcgcacctg gcccgcgatc tcggtcgtca
    12781 cgtcgacgcc gcgcaccggc gtcacgctgc cgacggccga cagctgaggc tgccattcca
    12841 gcggctccac cctggccgcg gtcaccacgc tgggcgtcgg cttcgggaag gaggacatca
    12901 gcttcttgat gtgcagcgcg aagccgccgg ccagtaccag caccagcagc agcacgcagc
    12961 caatcatgat gagcatccgc ttcgtcatgt cttactctcc agggtcgtgt tcgtgtgtgt
    13021 tcttcgcggg cgcgccatcc gtgtcgtgga cggcgcgccg gatctctttg aaacggggaa
    13081 ggcgggcctg cgcggcgccg ggctcagcgc gcggcgggcg cgctggcagc cgcggcggag
    13141 gccaccgcgt cgggcgcgtt ccaccagccg ccg
//
LOCUS       DASVLN010000226         6098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_495369, whole genome shotgun sequence.
ACCESSION   DASVLN010000226 DASVLN010000000
VERSION     DASVLN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495369"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VF453_05120"
     CDS             <1..849
                     /locus_tag="VF453_05120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEX7687061.1"
                     /translation="GLRPLQALSRAARDLRPKRRAQRLPDEHLPVELRQLADAFNGAL
                     VRQEEAYQQLEAFNADVAHELRTPLANMIGGTQVALARQRSAAEFQEALQSNLEELER
                     LRSIVNDMLFLARADQGEAATGVAQAELAREIELTIEFFEPLLDEHAMTVAVEGELRA
                     QATMNVALFRRALTNLLQNAIEHSADGARLVVAVTPRADSTWISVTNPGGTVAAEHLP
                     RLFDRFYRVDSARHDPGERHGHGLGLAIVRAVASMHGGAVSAASADGVTTVAFSLAAQ
                     PAQRPG"
     gene            complement(871..2424)
                     /locus_tag="VF453_05125"
     CDS             complement(871..2424)
                     /locus_tag="VF453_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7687062.1"
                     /translation="MEKKKLSVSARLRLMALATLLLAVMLSAYTGYAFYAASLDQRLA
                     TTRSFVAQSIAIAKRNADAAGSDPSAVAAAQAKALAEIRALRYDGNEYMWVNDMQARM
                     VMHPIKPEMNGKDLTDFKDPDGRRLFSDMVDIVRKSGHGYYDYLWPKPGSADPVSKRS
                     YVEGFAPWGWLIGSGVWTNDVRAAALRFAAINLAVGLAGGIAMLLFATSLARSMQRRL
                     QEVEAALQGLAAGDLTVSLHAERADEIGALVHAVERTRDGLQTVVSQVRHATDAITSA
                     SSEIATGNIDLSQRTEETSSNLQRTASSMTQLTGTVRQSADAASQANQLAHSASEVAT
                     RGGEVVSQVVTTMSDIAQASRKIADIIGVIDGIAFQTNILALNAAVESARAGEQGRGF
                     AVVAGEVRTLAKRSAEAAKEIKALIESSVSRVDTGSRLVSDAGETMHEIVSSVRRVSD
                     IVGEITAAASEQSAGIGVVNQAVGQLDQMTQQNAALVEESAAAAESLKSQAQKLSDVV
                     ATFRLAPAH"
     gene            complement(2700..4484)
                     /gene="ggt"
                     /locus_tag="VF453_05130"
     CDS             complement(2700..4484)
                     /gene="ggt"
                     /locus_tag="VF453_05130"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7687063.1"
                     /translation="MNPSRLQAPRRGARTALAAATIAALLAAALPTPVLAQLAPNAST
                     GVVRYDLVNEIFHPVHTQHGMVASESYLASQVGVDVLRKGGNAVDAAVATAFALAVTL
                     PNAGNLGGGGFMMLHDAKTGKDTAIDFREMAPGRASRNMYLDDKGDVIPDKSLFTQAA
                     IGVPGTVAGMMYALEHYGTKKRAELIAPAIALARKGFPVDEQLAVTLDAEQKHLGAWD
                     SSKKIFFKDGRPLRTGEILRNDDLANSMALISRDGARAFYEGPIGKAIVAEAKAHDGL
                     ITMADLKNYKVVERVPVRGSYRGYEIVSMPPPSSGGVHIVQILNMLSAFPLKEWGFNS
                     AKTIHAEAEAMKLAYADRSEYLGDPDFVKVPVKGLTSPAYAAELVKKIDPDKATPSAT
                     IKPGKPQPYESDQTTQFTIADAQGNVVSVTYTLNTSFGSGIVATGTGILLNNEMDDFS
                     AKPGVPNAYGLVGGDANAVGPMKRPLSSMSPTIVLKDGKPWLATGSPGGARIITTTLQ
                     VIVDMIDFGMNPAEASSAVRIHHQWLPDELRIEKGLSPDTIALLTGMGYKVVERPAMG
                     RTQTIEMTPAGFFGYSDPRNPNGATLGY"
     gene            4694..5836
                     /locus_tag="VF453_05135"
     CDS             4694..5836
                     /locus_tag="VF453_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7687064.1"
                     /translation="MPTRLPRFAAVVPAAAACLAAAFLALAAPLAAQAAGPEPKVLNI
                     YNWSDYIADDTLKNFEKETGIKVNYDNYDSNEILHGKLVAGNTGYDIVVPSAHFAKMQ
                     IEAGLFQKLDKSKLTNWGNLDPVLLQQMAKVDPGNQYLVDWLWGYITVGINTAKVKQA
                     LGGLAMPEDPFALVFDPKYADKLKSCGISFLDSPSEVLPIALGFIGKPPYSRNAADYK
                     AAGAMLKKVHADVGRFSSSGYINDMVAGSVCAVLGYSGDINIARARAIAAHNGNDIVA
                     FVPKHGATLFFDSMAIPKDAPHPQNALLFINYILRPEVHAALTNKVFYANPNKASLKY
                     VNKDVANNPSVFLKPEDLARMTVPDTLPQDIKRVQTRTFTEFKTGL"
BASE COUNT          841 a         2135 c         2177 g          945 t
ORIGIN      
        1 gggctgcggc cgctgcaggc gctgtcgcgc gcggcgcgcg acctgcggcc gaagcggcgc
       61 gcgcagcgcc tgcccgacga gcacctgccg gtcgagctgc ggcagctggc cgatgcgttc
      121 aacggcgcgc tcgtgcgcca ggaggaggcc taccagcagc tggaggcctt caacgccgac
      181 gtcgcgcacg agctgcgcac cccgctggcc aacatgatcg ggggcacgca ggtcgcgctg
      241 gcgcgccagc gcagcgccgc ggagttccag gaggcgctgc agtccaacct cgaggagctc
      301 gagcgcctgc gctccatcgt caacgacatg ctgttcctcg cccgcgccga ccagggcgag
      361 gccgcgaccg gcgtcgcgca ggcggagctc gcgcgcgaga tcgagctgac gatcgagttc
      421 ttcgagccgc tgctcgacga gcatgcgatg accgtggccg tcgagggcga gctgcgcgcg
      481 caggcgacga tgaacgtcgc gctgttccgg cgcgcgctca cgaacctgct gcagaacgcg
      541 atcgagcact cggccgacgg cgcgcgcctg gtggtcgcgg tcacgccgcg cgcggactcg
      601 acctggatct cggtgaccaa ccccggcggc accgtcgcgg cggagcacct gccgcgcctg
      661 ttcgaccgct tctaccgcgt cgactccgcg cgccacgacc cgggcgagcg ccatggccac
      721 ggcctcgggc tcgcgatcgt gcgcgcggtg gccagcatgc acggcggcgc ggtctccgcc
      781 gcgagcgccg acggcgtcac gacggtggcc ttcagcctcg ccgcgcagcc ggcgcagcgg
      841 ccgggctgag ctgccgcggc cgcgccggca tcaatgcgcc ggtgcgaggc ggaacgtcgc
      901 gacgacgtcc gagagcttct gcgcctgcga cttcagcgac tcggccgcgg cggcgctttc
      961 ctcgacgagc gcggcgttct gctgcgtcat ctggtcgagc tgccccactg cctggttgac
     1021 gacgccgatg ccggcgctct gctcgctggc ggcggcggtg atctcgccca cgatgtcgga
     1081 gacgcggcgc accgagctca cgatctcgtg catcgtctcg ccggcgtcgg agacgaggcg
     1141 gctgccggtg tccacgcgcg agacgctgct ctcgataagc gccttgatct ccttggcggc
     1201 ctcggcgctg cgcttggcca gcgtgcgcac ctcgcccgcc acgacggcga agccgcggcc
     1261 ctgctcgccg gcgcgcgccg actcgacggc cgcgttgagc gcgaggatgt tggtctggaa
     1321 cgcgatgccg tcgatcacgc cgatgatgtc ggcgatcttg cggctcgcct gggcgatgtc
     1381 gctcatcgtg gtgacgacct gcgagacgac ctcgccgccg cgcgtggcga cctcggaggc
     1441 cgagtgcgcg agctggttgg cctgcgaagc ggcatcggcg ctctggcgca cggtgccggt
     1501 gagctgcgtc atcgagctcg cggtgcgctg caggttgctc gaggtttcct cggtgcgctg
     1561 cgagaggtcg atgttgccgg tcgcgatctc ggacgatgcc gacgtgatgg cgtcggtcgc
     1621 gtggcgcacc tgcgagacga ccgtctgcag gccgtcgcgg gtgcgctcga cggcgtgcac
     1681 cagggcgccg atctcgtcgg cgcgctcggc atgcaggctg acggtcaggt cccccgcggc
     1741 caggccctgc agcgcggcct cgacctcctg cagccggcgc tgcatcgaac gcgccaggct
     1801 ggtggcgaac agcagcatcg cgatgccgcc ggccaagccg accgccaggt tgatcgccgc
     1861 gaagcgcagc gcggcggcgc gcacgtcgtt cgtccagacg cccgagccga tcagccagcc
     1921 ccagggcgcg aagccctcga cgtaggagcg cttggacacc gggtcggcgc tgcccggctt
     1981 cggccagagg tagtcgtagt agccgtggcc ggacttgcgc acgatgtcga ccatgtcgga
     2041 gaacaggcgg cggccgtccg gatccttgaa gtccgtgaga tccttgccgt tcatctccgg
     2101 cttgatcggg tgcatgacca tgcgcgcctg catgtcgttg acccacatgt actcgttgcc
     2161 gtcgtagcgc agcgcgcgga tctcggccag cgccttggcc tgcgcggcgg cgaccgcgga
     2221 cgggtcgctg ccggcggcgt cggcattgcg cttggcgatc gcgatcgact gcgcgacgaa
     2281 gctgcgcgtg gtcgcgagac gctggtcgag cgacgccgcg tagaacgcgt agccggtgta
     2341 cgcggacagc atcacggcca gtagcagcgt ggccagcgcc atcagccgta gccgcgcgga
     2401 gacggagagc ttcttctttt ccatcgggat tcccttctcg ttcgtccgcg cgggaggcgc
     2461 gcgcggacgg gccgtccgcg tcgcgccagg gcgatggggt ggccgcgcag gtccggtaga
     2521 tcggccggcg acgtgtccgg cgagaggcgg gtcgtcccgg accatgcccc ttttgcgtgc
     2581 acaaatgcgc accggcctgg ggtgcgcggt cgcgcagcgt cgcggcgtcg gcgggcgaag
     2641 catccgcgcc cggctgcccg gcacgcgcgc ggacgggctg ccgccgcgcg cgtgatcgct
     2701 cagtacccga gcgtcgcgcc gttcgggttg cgcgggtcgg agtagccgaa gaagccggcc
     2761 ggggtcatct cgatggtctg cgtgcggccc atcgccgggc gctcgacgac cttgtagccc
     2821 atgccggtca gcagcgcgat cgtgtcgggg ctcaggccct tctcgatgcg cagctcgtcg
     2881 ggcagccact ggtggtggat gcggacggcg ctcgaggcct cggccgggtt catgccgaag
     2941 tcgatcatgt cgacgatcac ctgcagcgtg gtcgtgatga tgcgggcgcc gcccgggctg
     3001 ccggtggcca gccacggctt gccgtccttg agcacgatcg tcggcgacat cgagctcagc
     3061 ggccgcttca tcggcccgac ggcgttggcg tcgccgccga ccaggccgta cgcgttgggc
     3121 acgccgggct tggcggagaa gtcgtccatc tcgttgttca gcaggatgcc ggtgccggtg
     3181 gcgacgatgc cggagccgaa gctcgtgttg agcgtgtagg tcacgctcac cacgttgccc
     3241 tgcgcgtcgg cgatcgtgaa ctgcgtggtc tggtcgctct cgtacggctg cggcttgccg
     3301 ggcttgatcg tcgccgaggg cgtcgccttg tccgggtcga tcttcttgac cagttccgcg
     3361 gcgtacgcgg gcgaggtcag ccccttgacc ggcaccttca cgaagtcggg gtcgccgagg
     3421 tactcggagc gatccgcata ggcgagcttc atcgcctcgg cctcggcgtg gatcgtcttc
     3481 gcgctgttga agccccactc cttgagcggg aaggccgaca gcatgttcag gatctgcacg
     3541 atgtgcacgc cgcccgagct cggaggcggc atcgagacga tctcgtagcc gcggtagctg
     3601 ccgcgcaccg gcacccgttc gacgaccttg tagttcttca ggtccgccat cgtgatcagc
     3661 ccgtcgtgcg ccttcgcctc ggcgacgatg gccttgccga tcgggccttc gtagaacgcc
     3721 cgggcgccgt cgcgcgagat cagcgccatg ctgttggcga ggtcgtcgtt ccgcaggatc
     3781 tcgccggtgc gcagcgggcg gccgtccttg aagaagatct tcttcgagct gtcccacgcg
     3841 ccgaggtgct tctgctcggc gtccagcgtg acggcgagct gctcgtcgac cgggaagccc
     3901 ttgcgcgcga gcgcgatggc cggcgcgatg agctcggcgc gctttttcgt gccgtagtgc
     3961 tcgagcgcgt acatcatgcc cgcgacggtg cccggcacgc cgatggccgc ctgcgtgaac
     4021 agcgacttgt cggggatgac gtcgcccttg tcgtccaggt acatgttgcg cgacgcgcgg
     4081 cccggggcca tctcgcggaa gtcgatcgcg gtgtccttgc cggtcttcgc gtcgtgcagc
     4141 atcatgaagc cgccgccgcc gaggttgccg gcgttgggca gcgtcacggc cagcgcgaag
     4201 gccgtcgcga ccgcggcgtc gaccgcgttg ccgcccttgc gcaggacgtc cacgccgacc
     4261 tgcgaggcga ggtagctctc gctcgccacc atgccgtgct gcgtgtgcac gggatggaag
     4321 atctcgttga cgaggtcgta gcgcacgacg ccggtggagg cgttcggcgc gagctgggcg
     4381 aggacggggg tgggcagcgc ggcggccagc agggccgcga tcgtggcggc ggccagtgcc
     4441 gtgcgggcgc cgcggcgggg agcctggaga cgggacggat tcatggcggg tgcatcctcc
     4501 gggggtggtg tggtcgagcc gcgatggtag tcccgctggc gtgcgggcgc cggtaccaat
     4561 ctccgtagtg gcgtggggcc cgccggtcgg gcccgtcgtg ccccggtcgc ccgcgcccgt
     4621 gggccgcggc cccgcgcgcc gtggtgcaat acgggttgtc gccgttccac ccctgcccgg
     4681 agcctcccgc cccatgccga cccgcctgcc tcgcttcgcc gccgtcgttc ccgccgcggc
     4741 cgcctgcctc gccgctgcct tcctcgccct cgccgcgccc ctggccgcgc aggccgccgg
     4801 gccggagccg aaggtgctca acatctacaa ctggtcggac tacatcgccg acgacacgtt
     4861 gaagaacttc gagaaggaaa cgggcatcaa ggtcaactac gacaactacg acagcaacga
     4921 gatcctgcac ggcaagctgg tcgcgggcaa cacgggctac gacatcgtcg tgccgagcgc
     4981 gcacttcgcc aagatgcaga tcgaggccgg cctgttccag aagctcgaca agagcaagct
     5041 gaccaactgg ggcaacctcg acccggtgct gctgcagcag atggccaagg tcgacccggg
     5101 caaccagtac ctcgtcgact ggctgtgggg ctacatcacg gtcggcatca acaccgccaa
     5161 ggtcaagcag gcgctcggcg ggctggcgat gcccgaggat ccgttcgcgc tggtcttcga
     5221 cccgaagtac gccgacaagc tgaagagctg cggcatcagc ttcctcgact cgccctccga
     5281 ggtgctgccg atcgcgctcg gcttcatcgg caagccgccc tacagccgca acgcggccga
     5341 ctacaaggcc gccggcgcga tgctgaagaa ggtgcatgcc gacgtcggcc gcttctcgtc
     5401 gtcgggctac atcaacgaca tggtcgcggg ctcggtgtgc gcggtcctcg gctactcggg
     5461 cgacatcaac atcgcccgcg cccgcgcgat cgcggcccac aacggcaacg acatcgtcgc
     5521 cttcgtgccc aagcacggcg cgacgctgtt cttcgactcg atggcgatcc ccaaggacgc
     5581 gccgcatccg cagaacgcgc tgctgttcat caactacatc ctgcgccccg aggtgcacgc
     5641 ggcactgacc aacaaggtct tctacgcgaa cccgaacaag gcgtcgctga agtacgtcaa
     5701 caaggacgtc gcgaacaacc cgagcgtgtt cctcaagccc gaggacctcg cccgcatgac
     5761 ggtgcccgac acgctgccgc aggacatcaa gcgcgtgcag acccgcacct tcacggagtt
     5821 caagaccggg ctctgacggc cgaattgggc ggcctcgggg ccgctgttcg ggctttagga
     5881 cggcgcgacc ctgcctacga tggaggggcg atggacgccg gttgaccggc gtgtccgccg
     5941 gacggcccga ccgcgggcgc atcgcgaccc gactgtccag gaatcccgtc atgcgcgtcc
     6001 aagccaacca ctcctccgca acgtccctgc cgggcctgtc gtccggcggc ggcaaggccg
     6061 ggcacgcggg ccacgccggc aaggccgcgg cggcggcc
//
LOCUS       DASVLN010000227         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_280268, whole genome shotgun sequence.
ACCESSION   DASVLN010000227 DASVLN010000000
VERSION     DASVLN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280268"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..2111)
                     /locus_tag="VF453_05140"
     CDS             complement(204..2111)
                     /locus_tag="VF453_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7687065.1"
                     /translation="MTAPRTGALLRTSVPLPFVLRRGARALLATLPALAAGSACAQSS
                     AGDVPAQDVVVTAARTAQKLPDTLPATTVISRHDIEASPATDLPDLLRQMTSFGVAQT
                     GPLGSQTSVFLRGANSNQVLVLVDGAPLSRADFGSAPWELIPLGQIDHVEIVRGNLSS
                     LYGASAVGGVVQIFTRKGAGASVSVGVGGNGHVETSGSIGRRLGDAATPLELSASLSG
                     QNTDGYSARDPKADPTANPDRDPGYQAGATVSIGKTWAPGQRTDFSVMHSYTHSDYDG
                     YTKHVGQDTLTTDLDEFSLRSRHQLATGLAMNLSAGQTLEHFRDPTEADGEFGASPTV
                     GSGRTRLLGAELDWQAAPDHQVQLQFEDRDERFGEYPNAQQSRRTKSERLGYLGRVAG
                     VVEVQANLRHDDADDFGSADTGLLALGWNVTQALKLVGQFSTAFSAPSFTELEYAVSP
                     DALKAERSRDLEFGAHWQGRGWLARATWFSQHQRDLVGFNAAFQSVNIGRATNRGVEL
                     AADGDTGLGKVGLDATFQNPRDGDTDTQLLRRPRTVVAASWRVPLMGWESGVYVRYAG
                     RHLDADPVTFADVEAKARTTLGLSTQHALSPNWTLGAKVDNVANTRTPEVLGYTAPRR
                     ELLFTLRGTWQ"
     regulatory      complement(2190..2452)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2535..2807
                     /locus_tag="VF453_05145"
     CDS             2535..2807
                     /locus_tag="VF453_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HEX7687066.1"
                     /translation="MSRLPDLAERIERLLLRHEEQKRTNQLLEQQLRNVTDERDNLRS
                     RLAAARARIDALIDRLPGAEADAAAAIASTEVADADAKTGNGGTTS"
     gene            2804..>3039
                     /locus_tag="VF453_05150"
     CDS             2804..>3039
                     /locus_tag="VF453_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX7687067.1"
                     /translation="MKQVEVTILGQSYLLACPDGGEHLLHAAVAAVDREMSAIRDAGK
                     VKARERIAVLAALNLAYRLAELPRGAAAEAAAEAT"
BASE COUNT          415 a         1072 c         1150 g          402 t
ORIGIN      
        1 gcgagcacgc ggggcgcgcg cagcgtctcg atgaggcgga cgtcccagcc gcccgcgccc
       61 aggcacaggg cgagtgccgc gatcgcggcg gtggcgagcg ccacgcacgc gagccgcgcg
      121 atcgccggat cgcgcacggc cggggcatcg ggggtcgaca tcgatgcagt gtcggtcatc
      181 gcggcccccc gttcacggcg gcgtcactgc cacgtcccgc gcagcgtgaa cagaagctcg
      241 cggcgcggcg cggtgtagcc gaggacttcg ggcgtgcgcg tgttggcgac gttgtcgacc
      301 ttggcgccga gggtccagtt cggcgacagc gcatgctggg tcgacagccc gagcgtcgtg
      361 cgcgccttgg cctcgacgtc ggcgaaggtc accggatcgg cgtcgaggtg ccggcccgcg
      421 tagcgcacgt acacgcccga ctcccagccc atcagcggca cgcgccagct ggcagcgacg
      481 accgtgcgcg ggcggcgcag cagttgcgtg tcggtgtcgc cgtcgcgcgg gttctggaac
      541 gtggcgtcga ggcccacctt gcccaggccg gtgtcgccgt cggcggccag ctcgacgccg
      601 cggttggtcg cacggccgat gttgaccgac tggaacgccg cgttgaagcc gacgaggtcg
      661 cgctggtgct gcgagaacca ggtcgcgcgc gccagccagc cgcggccctg ccagtgcgcg
      721 ccgaactcga ggtcgcgcga gcgctcggcc ttcagcgcgt cgggggagac cgcgtactcg
      781 agctcggtga agctcggcgc ggagaacgcg gtggagaact gtcccaccag cttcagcgcc
      841 tgcgtgacgt tccagcccag cgccagcagg ccggtgtcgg ccgagccgaa gtcgtccgcg
      901 tcgtcgtgac gcagattggc ctgcacctcg acgaccccgg cgacgcgacc caggtagccc
      961 aggcgctcgg acttcgtccg gcgcgactgc tgcgcgttcg ggtactcgcc gaagcgctcg
     1021 tcgcgatcct cgaactggag ctgcacctgg tggtcgggcg cggcctgcca gtccagctcg
     1081 gcgccgagca ggcgggtgcg gccggagccc accgtcggac tcgcgccgaa ctcgccgtcg
     1141 gcctcggtcg ggtcgcggaa gtgctccagc gtctggccgg cgctcaggtt catcgcgagg
     1201 ccggttgcga gctggtggcg cgatcgcagc gagaactcgt cgaggtcggt ggtcagcgtg
     1261 tcctggccga cgtgcttcgt gtagccgtcg tagtcgctgt gcgtgtagct gtgcatgacg
     1321 ctgaagtcgg tgcgctggcc gggcgcccag gtcttgccga tcgagaccgt ggcgcccgcc
     1381 tggtagcccg ggtcgcggtc ggggttggcc gtcgggtcgg ccttcggatc gcgtgcgctg
     1441 tagccgtcgg tgttctggcc cgacaggctc gcgctcagct ccagcggcgt cgcggcgtcg
     1501 ccgaggcggc ggccgatcga gccgctcgtc tcgacgtggc cgttgccgcc cacgcccacc
     1561 gagacgctgg cgccggcgcc cttcctcgtg aagatctgca ccacgccgcc gacggcggac
     1621 gcgccgtaca ggctcgagag gttgccgcgc acgatctcga catggtcgat ctggcccagc
     1681 gggatcagct cccagggcgc ggagccgaag tccgcgcgcg acagcggcgc gccgtcgacg
     1741 agcacgagca cctggttgga gttcgcgccg cgcaggaaca cgctggtctg cgagccgagg
     1801 gggccggtct gcgcgacgcc gaagctggtc atctggcgca gcaggtcggg caggtcggtg
     1861 gcgggcgagg cctcgatgtc gtggcggctg atgacggtcg tggcgggcag cgtgtcgggc
     1921 agcttctgcg cggtgcgcgc cgcggtgacg acgacgtcct gcgcggggac gtcgcccgcg
     1981 ctggattgcg cgcaggcgct gccggcggcc agcgccggca gcgtggcgag caacgcgcgc
     2041 gcgccgcggc ggaggacgaa cggaagggga acggacgtgc gcagcagcgc gccggtgcgg
     2101 ggggcagtca tgataggaaa cgagggtgag ccagcgtggc gcgagcttcc ccgcacgcgc
     2161 caccgaacgg cccgccccgc gatgcgcgtg cgacgggccc cgaggttggc cggtatccgg
     2221 gctggcggag aggcagcgcc cgaaggcctt cccatgcgcc ttgggaggac gcacagtgac
     2281 cacgggtgtc ggacgccgat gaagcctcgt ggcgaggccg tcatcctccg cgaccgttgc
     2341 gggggcagct caggtggggc cggcgcttcg aggcgaaggc ggccgtcctg attcccgttt
     2401 gactcgggcg gcgaggaacg ccgcccggag caccaactga agagcggatc gtaccattcg
     2461 acaccgcctt ccccccgcgg agcccctcta caatcggctt ttccgccgcg cgtcccccga
     2521 cgccccaagc ctccatgtca cgactgccgg atcttgcgga acgcatcgag cgactcctgc
     2581 tgcgccacga ggaacagaag cgcaccaacc agctgctcga gcagcagctg cgcaacgtca
     2641 ccgacgagcg cgacaacctg cgctcgcggc tcgccgcggc gcgcgcgcgc atcgacgcgc
     2701 tgatcgaccg gctgcccggc gccgaggccg atgcggccgc ggcgatcgcg agcaccgagg
     2761 tcgcggacgc ggatgcgaag accggcaacg gaggcacgac gtcatgaagc aggtggaagt
     2821 caccatcctc ggccagagct acctgctcgc ctgccccgac ggcggcgagc acctgctgca
     2881 cgccgccgtc gccgccgtcg accgcgagat gagcgccatc cgcgacgccg gcaaggtgaa
     2941 ggcgcgcgag cgcatcgcgg tgctggcggc cctgaacctc gcctaccggc tcgccgagct
     3001 gccccgcggc gccgcggccg aggccgccgc cgaggcgac
//
LOCUS       DASVLN010000228         5678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_280392, whole genome shotgun sequence.
ACCESSION   DASVLN010000228 DASVLN010000000
VERSION     DASVLN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280392"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VF453_05155"
     CDS             complement(<1..259)
                     /locus_tag="VF453_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687068.1"
                     /translation="MTSPAASRRPQRLCILGSTGSIGTSTLDVVALHPADYEVFALTA
                     QSRVDELFAQLVRWRPRYAALPDAVAADALGARVKAAGLPTE"
     gene            complement(267..1094)
                     /locus_tag="VF453_05160"
     CDS             complement(267..1094)
                     /locus_tag="VF453_05160"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7687069.1"
                     /translation="MLRERVLTALVLLALLVPAVAADNPLWFELLTVLMIGAAGWEWA
                     RLCGLGQASASVSGVALALACAVLGPAYEIEIPPVAWSGVTAVWVLGGAIALRAGPDG
                     FKRLNHAVRFIAGWLLIGAAWAALIASRARGLPYLASVCGLVWMADIAAYFGGRALGR
                     RKLAPTISPGKSWEGAISGLVGVLVLAVVVVVVTARPHGNLFWVLRTHLGWAGMIVAC
                     AALVALAVTGDLVESLVKRAAGMKDSSRLLPGHGGVLDRIDALLPVFPAALALVTAF"
     gene            complement(1084..1851)
                     /gene="uppS"
                     /locus_tag="VF453_05165"
     CDS             complement(1084..1851)
                     /gene="uppS"
                     /locus_tag="VF453_05165"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX7687070.1"
                     /translation="MTASVFPSGAARRDPSVPRHVAIVMDGNGRWAKKRFMPRIFGHK
                     HGVDALVATIQACANRGIEHLTVFAFSSENWKRPEEEVSGLMGLVLSAMTRYLTKIAG
                     DGVRVRIIGDRSAVSASVRAAWERAEKTTAHNDRITVWVAFNYGGRWDIVQACQRAIA
                     DGVPASELTEERLSSYMALREAPDPDLFIRTGGEVRISNFLLWQVAYSELYFTQCLWP
                     DFDEAELDRALSAFAARDRRFGGVKPDAVATAGGNAA"
     gene            complement(1866..2426)
                     /gene="frr"
                     /locus_tag="VF453_05170"
     CDS             complement(1866..2426)
                     /gene="frr"
                     /locus_tag="VF453_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7687071.1"
                     /translation="MTIADIKKNAETKMARSIEAFKTELQKIRTGRAHAGLLDQIHVE
                     YYGSDVPLNQVANISLLDARTIGVQPFEKQMASKIEKAIRESDLGLNPSTTGDTIRVP
                     MPMLTEERRKDLTKVVRHQGEESKVAVRNLRREANDGAKKLLKDKEITEDEERRLSDD
                     VQKLTDRTIAEIDRLVQAKEAEILAV"
     gene            complement(2523..3233)
                     /gene="pyrH"
                     /locus_tag="VF453_05175"
     CDS             complement(2523..3233)
                     /gene="pyrH"
                     /locus_tag="VF453_05175"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7687072.1"
                     /translation="MPAYKRILLKLSGEALMGDDAYGINRATIVRMVREVQEVVRQGT
                     EVAVVIGGGNIFRGVAGGSVGMDRATADYMGMLATVMNALAIADTMRQEGMVARVMSA
                     IGIDQVVEPYVRPKALQYLEEGKVVVFGAGTGNPFFTTDTAAALRGAEIGAEIVLKAT
                     KVDGVYSADPKKVPDATRYTSITFDEAITRNLQVMDATAFALCRDQNLPIKVFSIFKP
                     GALARVVAGEDEGTLVHV"
     gene            complement(3385..4260)
                     /gene="tsf"
                     /locus_tag="VF453_05180"
     CDS             complement(3385..4260)
                     /gene="tsf"
                     /locus_tag="VF453_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7687073.1"
                     /translation="MPAITASMVAELRAKTDAPMMLCKKALTEADGDLAKAEEILRVK
                     LGTKASNAASRVTAEGVIATYVAGNVGAMVEVNCETDFVTKNEEFIAFANKLAKLVAE
                     KNPADVAALSALELEGTTVDAFRLALVGKIGENMSIRRFERFENAGLAHYLHGTRIGV
                     VVQFEGDDVAAKDVAMHVAATKPKALSAADVPADLVETERNVATQKAAESGKPADIVA
                     KMVEGSVQKYLKEVSLLNQSFVKNDKQTVEQMLKAANTTIKGFTLYVVGEGIEKKVDN
                     FADEVAAQVAAARGQ"
     gene            complement(4430..5176)
                     /gene="rpsB"
                     /locus_tag="VF453_05185"
     CDS             complement(4430..5176)
                     /gene="rpsB"
                     /locus_tag="VF453_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7687074.1"
                     /translation="MSVSMREMLEAGVHFGHQTRFWNPKMAPYIYGHRNKIHIINLEK
                     TLPAFENALKFVRQLAAKRGTILMVGTKRQAREVVAAEAQRCGMPYVDQRWLGGMLTN
                     FKTVKGSLKKLKDMQAATEAGTVAVIKKEALMFQRERLKLEKDIGGIQDMAALPDAIF
                     VIDVGYHKIAILEANKLGIPVIGVVDTNHSPQGIDYVIPGNDDSAKAVALYAKAIADA
                     VLEGKANATNEVAQQVSAAGDEFVEVSAEG"
     gene            5454..>5678
                     /locus_tag="VF453_05190"
     CDS             5454..>5678
                     /locus_tag="VF453_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7687075.1"
                     /translation="MKVAVIGAGMAGVAAAHALARDGHDVTVLERRASVAEETSFAPA
                     GIAAAGLAAPWLPMPGRLQASLSGGEVAALR"
BASE COUNT          810 a         1974 c         1951 g          943 t
ORIGIN      
        1 cctcggtcgg caggcccgcg gccttcacgc gcgcgccgag cgcgtcggcg gcgaccgcat
       61 cgggcagcgc cgcgtagcgc ggccgccagc gcacgagctg cgcgaacagc tcgtcgacgc
      121 ggctctgcgc ggtcagcgcg aagacctcgt agtccgcggg atgcagcgcg acgacgtcca
      181 gcgtgctggt gccgatggag ccggtcgagc cgaggatgca caggcgctgc ggccggcggc
      241 tcgcggcagg ggaagtcatg ggcatgtcag aaggccgtca cgagggccag ggccgccggg
      301 aacacgggca gcagcgcgtc gatgcgatcc agcacgccgc cgtggcccgg cagcaggcgg
      361 ctcgaatcct tcatgccggc ggcgcgcttg accagcgact cgacgaggtc gcccgtcacc
      421 gccagcgcca ccagcgccgc gcacgcgacg atcatgccgg cccagcccag gtgcgtgcgc
      481 agcacccaga acaggttgcc gtgcgggcgc gcggtgacga ccaccacgac gaccgccagc
      541 acgagaacgc ccacgaggcc gctgatcgcg ccctcccagc tcttgccggg gctgatggtc
      601 ggcgcgagct tgcgccggcc gagcgcccgg ccgccgaagt aggccgcgat gtcggccatc
      661 cagacgaggc cgcagaccga tgccagatac ggcagcccgc gcgcacgcga ggcgatcagc
      721 gcggcccacg cggccccgat cagcagccag cctgcgatga agcgcacggc gtggttcagg
      781 cgcttgaagc cgtcggggcc ggcacgcagc gcgatcgcgc cgccgagcac ccagaccgcg
      841 gtcacgccgc tccaggcgac gggcgggatc tcgatctcgt aggccggacc gagcaccgcg
      901 caggccagcg cgagcgcgac gcccgagacg cttgccgacg cctgcccgag gccgcacagc
      961 cgtgcccact cccagccggc ggcgccgatc atcagcaccg tcagcagctc gaaccacagc
     1021 ggattgtccg cggcgaccgc gggcacgagc agcgccagca gcaccagcgc ggtcaggacg
     1081 cgctcacgca gcattgccgc cggccgtcgc gacggcatcc ggtttcacgc cgccgaagcg
     1141 acggtcgcgt gccgcgaagg ccgacagcgc gcggtcgagc tcggcctcgt cgaagtccgg
     1201 ccagaggcac tgcgtgaagt acagctccga gtaggccacc tgccagagca ggaagttgct
     1261 gatgcgcact tcgccccccg ttcggatgaa gaggtcggga tccggcgcct cgcgcagcgc
     1321 catgtacgag gacaggcgct cctcggtcag ctcggacgcg ggcacgccgt cggcgatcgc
     1381 gcgctggcag gcctggacga tgtcccagcg gccgccgtag ttgaaggcga cccacacggt
     1441 gatgcggtcg ttgtgcgcag tcgtcttctc ggcgcgctcc caggccgcgc gcacggacgc
     1501 cgagaccgcg ctgcggtcgc cgatgatgcg cacgcgcacg ccgtcgccgg cgatcttggt
     1561 caggtagcgc gtcatggcgc tcagcaccag gcccatgagg ccggagacct cctcctcggg
     1621 gcgcttccag ttctccgacg agaacgcgaa gacggtcagg tgctcgatgc cgcggttggc
     1681 gcaggcctgg atcgtcgcga cgagggcgtc gacgccgtgc ttgtggccga agatacgcgg
     1741 catgaagcgc ttcttcgccc agcggccgtt gccgtccatc acgatggcga cgtggcgcgg
     1801 cacggacggg tcgcgtcgcg cggcgccgga cgggaacacg gacgcggtca cttttcgctt
     1861 cgggatcaaa ccgcgaggat ctcggcttcc ttggcctgca ccagccggtc gatctccgcg
     1921 atggtgcgat cggtcagctt ctgcacgtcg tcggagaggc ggcgctcctc gtcctcggtg
     1981 atctccttgt ccttgagcag cttcttggcg ccgtcgttgg cctcgcggcg caggttgcgc
     2041 acggcgacct tggactcctc gccctggtgg cgcacgacct tggtcaggtc cttgcggcgc
     2101 tcctcggtga gcatgggcat cggcacgcgg atcgtgtcgc cggtcgtcga cgggttcagg
     2161 cccaggtcgg actcgcggat ggccttctcg atcttggagg ccatctgctt ctcgaacggc
     2221 tgcacgccga tcgtgcgcgc gtccagcagc gagatgttgg cgacctggtt cagcggcacg
     2281 tccgagccgt agtactcgac gtggatctgg tccagcaggc ccgcgtgcgc gcgcccggtg
     2341 cggatcttct gcagctcggt cttgaacgcc tcgatggaac gggccatctt ggtctcggcg
     2401 ttcttcttga tgtcggcaat cgtcatgatc tctcaacgct cctcacaacc tgggacggcc
     2461 gccgcttgcc acgacgcgcc gtcgctcgaa aactcgtgcg cgcccgcacg ggacgcgcac
     2521 gctcagacgt gcaccagcgt gccctcgtcc tcgcccgcca cgacgcgcgc cagcgcgccc
     2581 ggcttgaaga tgctgaacac cttgatcggc aggttctggt cgcggcacag cgcgaaggcg
     2641 gtcgcgtcca tcacctgcag gttgcgggtg atggcctcgt cgaacgtgat ggacgtgtag
     2701 cgggtcgcgt cgggcacctt cttcgggtcg gcgctgtaga cgccgtccac cttggtcgcc
     2761 ttcagcacga tctcggcgcc gatctcggcg ccgcgcagcg cggcggcggt gtcggtggtg
     2821 aagaacggat tgccggtgcc ggcgccgaag acgacgacct tgccctcttc caggtactgc
     2881 agcgccttgg gccgcacgta gggctcgacc acctggtcga tgccgatcgc cgacatcacg
     2941 cgggcgacca tgccctcctg gcgcatcgtg tcggcgatcg cgagcgcgtt catcaccgtg
     3001 gcgagcatgc ccatgtagtc ggccgtcgcg cgatccatgc ccaccgagcc gcccgcgacg
     3061 ccgcgaaaga tgttgccgcc gccgatcacg accgcgacct cggtgccctg gcgcacgact
     3121 tcctgcacct cgcgcaccat gcgcacgatc gtggcgcggt tgatgccgta ggcgtcgtcg
     3181 cccatcagcg cttcgccgga gagtttcaga aggatgcgct tgtaggccgg cataggatga
     3241 gggactcccc gtggtgcgcg cggtcagcgc ctggacgttc gttgttcgat cccgcagtgt
     3301 aagccggcgc cgcgcgtgcg ccggccgtcc ccgtgcggtc ggggtggcgg gggccggcgc
     3361 tcgtgacgcc ggccctcgcg ggcctcactg gccgcgggcg gcggccacct gggcagccac
     3421 ttcgtcggcg aagttgtcga ccttcttctc gatgccctcg cccacgacgt agagcgtgaa
     3481 gcccttgatg gtcgtgttgg cggccttgag catctgctcg acggtctgct tgtcgttctt
     3541 cacgaacgac tggttcagca gcgagacttc cttcaggtac ttctgcaccg agccctcgac
     3601 catcttggcg acgatgtcgg ccggcttgcc cgactcggcg gccttctgcg tggcgacgtt
     3661 gcgctcggtc tcgaccaggt cggccgggac gtccgcggcc gacagtgcct tcggcttcgt
     3721 cgcggcgacg tgcatcgcga cgtccttggc ggccacgtcg tcgccctcga actggacgac
     3781 cacgccgatg cgcgtgccgt gcaggtagtg cgcgaggccg gcgttctcga agcgctcgaa
     3841 gcggcggatg gacatgttct cgccgatctt gccgaccagc gcgaggcgga aggcgtcgac
     3901 ggtcgtgccc tcgagctcga gcgcggacag cgcggcgacg tcggcggggt tcttctcggc
     3961 gaccagcttg gccagcttgt tggcgaaggc gatgaactcc tcgttcttcg tgacgaagtc
     4021 ggtctcgcag ttgacctcga ccatcgcgcc gacgttgccg gcgacgtagg tggcgatcac
     4081 gccctcggcc gtgacgcgcg aggcggcgtt gctcgccttg gtgcccagct tgacgcgcag
     4141 gatctcttcc gccttggcca ggtcgccgtc ggcttcggtc agcgccttct tgcagagcat
     4201 catcggcgca tcggtcttgg cgcgcagttc agccaccatg cttgcggtaa tcgcgggcat
     4261 cttctaactc catcatctgc gccggccagg gtagtggccg acagtgaaca aattgcttct
     4321 gtcgagtcgg gcaggcggca cgccgcccga ctcatccctg cggcgaccca actgtgcgaa
     4381 gggggcacga agcccccttg cagatcgaac cgtccgggga gcggacgcgt cagccttcgg
     4441 cgctgacctc gacgaactcg tcgccggccg cggagacctg ctgcgcgacc tcgttggtgg
     4501 cgttggcctt gccctcgagc accgcgtcgg cgatcgcctt ggcgtacagc gcgacggcct
     4561 tggccgaatc gtcgttgccc gggatcacgt agtcgatgcc ctgcggcgaa tggttggtgt
     4621 cgacgacgcc gatgaccggg atgcccagct tgttggcctc gaggatggcg atcttgtgat
     4681 agccgacgtc gatcacgaag atcgcgtccg gcagcgcggc catgtcctgg atgccgccga
     4741 tgtccttctc cagcttcagg cgctcgcgct ggaacatcag cgcttccttc ttgatgacgg
     4801 cgaccgtgcc ggcctcggtg gcggcctgca tgtccttcag cttcttcagc gaacccttga
     4861 ccgtcttgaa gttggtcagc atgccgccga gccagcgctg gtcgacgtag ggcatgccgc
     4921 agcgctgcgc ttcggccgcg acgacttcgc gcgcctggcg cttggtgccc accatcagga
     4981 tcgtgccgcg cttggccgcc agctggcgga cgaacttcag cgcattctcg aacgccggca
     5041 gcgtcttctc gagattgatg atgtggatct tgttgcgatg gccgtagatg tacggggcca
     5101 tcttggggtt ccagaagcgc gtttggtggc caaaatgcac accggcttcc agcatttcgc
     5161 gcatggagac ggacattggg gtaactccga aggttgggtc tagaatcccg gctcactttg
     5221 catcgagtca cggcgcttac ggttgctgac catcggtccc accgctcgag tcgccgacct
     5281 cggacacaac accttgtctg ccgggttcgc gggtttggga cttcacatga agccccgttc
     5341 ttgccgagca ccgcgcgttg ccgcatggcc acccagaaaa acccaaagat tatagcaggc
     5401 cgcgagcggc cttgtcaccc gcccctgtcc gccgtccagg cgcgggccgt gcgatgaagg
     5461 tggccgtgat cggcgccggc atggccggcg tcgccgccgc gcatgcgctc gcgcgcgacg
     5521 gccacgatgt caccgtcctc gaacgtcgcg ccagcgtggc cgaggagacg agtttcgcgc
     5581 ccgcgggcat cgcggccgcc ggcctcgcgg cgccctggct gccgatgccc ggtcgcctgc
     5641 aggcgagcct ctcgggcggc gaggtcgccg cgctgcgg
//
LOCUS       DASVLN010000229         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_495640, whole genome shotgun sequence.
ACCESSION   DASVLN010000229 DASVLN010000000
VERSION     DASVLN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495640"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1546)
                     /locus_tag="VF453_05195"
     CDS             complement(<1..1546)
                     /locus_tag="VF453_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEX7687076.1"
                     /translation="MHPTDRRKFLKTAAAAAAVGANALPAGIRQALAIPAHRETGTIE
                     DVKHIVILMQENRSFDHYFGTMRGVRGFGDPRPATLPGGKSVWHQPGNGGEVLPFRPD
                     VPNLGLQFLEGTPHDWTSMHQAWNQGRLDQWVPAKGANTMAYLQREDIPFHFALADAF
                     TICDAYHCSLLGPTDPNRYHMWTGWVGNDGSGGGPVVDNAEAGYGWSTFPERLTNAGI
                     SWKVYQDVGTGLDAAGFWGWTSNPYIGNYGDNSLLYFHQYQDAQPGSPLYEGARRGTN
                     VAADPGQSFFDLLAADVAAGTLPQVSYIVAPEAYSEHPNWPANYGAWYTSQVLEVLTS
                     NPEVWSKTALFVTFDENDGLFDHVTPPFPALSRAGGLSTADVSRESFAGSASYPAGPY
                     GLGARVPMTIVSPWTKGGWVCSQVFDHTSLIRFVETRFGRAHPNLRESNITPWRRAVT
                     GDLTSAFNFADPNGEVGHLPSTAAYLPADTNRHDSVVPVPPADQAVPRQEPGLRRSRA
                     LPYAFEV"
     gene            complement(1739..>2126)
                     /locus_tag="VF453_05200"
     CDS             complement(1739..>2126)
                     /locus_tag="VF453_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687077.1"
                     /translation="NAGETAIDGVESAGSEVAGVVTGTYGALKGAVGAVVGGVEHGVE
                     AVVHGVEDVVEGSWNLATQGVQTVVDGIGTAAHEVFIDAPAAIVSTAASVAEDVASAA
                     VKVAGAAVEYSASPLVVGAKLVASVI"
BASE COUNT          288 a          736 c          791 g          311 t
ORIGIN      
        1 cgacctcgaa cgcgtagggc agcgcgcgcg agcggcgcag gccgggctcc tggcgcggca
       61 cggcctggtc ggccggcggc accggcacga cgctgtcgtg gcggttcgtg tcggccggca
      121 ggtaggcggc ggtcgagggc aggtggccga cctcgccgtt cgggtcggcg aagttgaagg
      181 cggaggtcag gtcgcccgtg accgcgcggc gccagggcgt gatgttggac tcgcgcaggt
      241 tcggatgcgc gcggccgaag cgggtctcga cgaagcggat gagcgacgtg tggtcgaaca
      301 cctgcgagca cacccagccg cccttggtcc acggcgagac gatggtcatg ggcacgcgcg
      361 cacccaggcc ataggggccg gccgggtagc tcgcgctgcc ggcgaacgat tccctggaga
      421 cgtccgccgt cgacaggccg ccggcgcgcg acagtgccgg gaacggcggc gtgacgtggt
      481 cgaacaggcc gtcgttctcg tcgaaggtga cgaacagggc cgtcttgctc cacacctcgg
      541 gattggaagt cagcacctcc agcacctgcg aggtgtacca ggcgccgtag ttggccggcc
      601 agttcgggtg ctcggagtac gcctccggcg cgacgatgta ggagacctgc ggcagcgtgc
      661 ccgcggcgac gtcggcggcc aggaggtcga agaacgactg cccgggatcg gccgcgacgt
      721 tggtgccgcg gcgcgcgccc tcgtacagcg ggctgccggg ctgggcgtcc tggtactggt
      781 ggaagtacag cagcgagttg tcgccgtagt tgccgatgta cgggttgctc gtccagcccc
      841 agaagccggc ggcgtccagg ccggtcccga cgtcctggta gaccttccac gagatgcccg
      901 cgttggtgag gcgctcgggg aaggtcgacc agccgtagcc cgcctccgcg ttgtcgacga
      961 ccgggccgcc gcccgatccg tcgttgccga cccagcccgt ccacatgtgg tagcggttcg
     1021 ggtcggtggg cccgagcagc gagcagtggt aggcgtcgca gatcgtgaac gcgtcggcga
     1081 gcgcgaagtg gaacgggatg tcctcgcgct gcaggtacgc catcgtgttg gcgcccttgg
     1141 ccggcaccca ctggtcgagg cgcccctggt tccaggcctg gtgcatcgac gtccagtcgt
     1201 gcggcgtgcc ctcgaggaac tgcaggccga ggttgggcac gtccgggcgg aacgggagca
     1261 cctcgccgcc gttgcccggc tggtgccaca ccgacttgcc gcccggcagc gtcgccggcc
     1321 gcgggtcgcc gaatccgcgc acgccgcgca tcgtgccgaa gtagtggtcg aacgaccggt
     1381 tctcctgcat gaggatgacg atgtgcttga cgtcctcgat ggtgcccgtc tcgcggtgcg
     1441 cggggatggc cagcgcctgg cggatgcccg cgggcagcgc gttggcgccg acggcggccg
     1501 cggcggcggc ggtcttgagg aacttgcggc gatcggtggg atgcatggag tccgtcctgt
     1561 cggtttgtcg ttgtcgcacg tccgcaccgc gggcgtggct ggcgggcaat ctaggacgac
     1621 cgcatgacaa cccgaagaca cgcgcggcgt cttccgaagg acggatacaa cccttacgcg
     1681 cgcggacgct tccgcgtcgc gcgccaaggt ccgccgcgcc tcccggcgcc ggccggcctc
     1741 agatgacgga cgcgacgagc ttggcgccga ccacgagcgg cgatgcgctg tactcgacgg
     1801 cggccccggc caccttcacc gcggcgctgg ccacgtcctc ggccacgctc gcggccgtcg
     1861 acacgatggc ggccggcgcg tcgatgaaca cctcgtgcgc ggccgtgccg atgccgtcga
     1921 cgacggtctg cacgccttgc gtggcgaggt tccagctgcc ttccacgaca tcctcgacgc
     1981 cgtgcaccac ggcctccacg ccatgctcga ccccgccgac cacggcgccg accgcgccct
     2041 tgagcgcgcc gtaggtgccg gtcacgacgc cggcgacctc cgagcccgcg ctctcgacgc
     2101 catcgatcgc ggtctcgccc gcgttc
//
LOCUS       DASVLN010000230         9202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_64715, whole genome shotgun sequence.
ACCESSION   DASVLN010000230 DASVLN010000000
VERSION     DASVLN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64715"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VF453_05205"
     CDS             <1..191
                     /locus_tag="VF453_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEX7687078.1"
                     /translation="PAPAAYEPLLQRVFAGFADSAAPVTRAQDVAEAVWRAATDAACP
                     MFLPAGADAVAATEAAGQ"
     gene            complement(298..3120)
                     /locus_tag="VF453_05210"
     CDS             complement(298..3120)
                     /locus_tag="VF453_05210"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7687079.1"
                     /translation="MPSASAGTADFRKLFEASPGSYLALRADGPRFTIVAVTDAYLQA
                     TMTRREDIVGRPLFEVFPDNPQDPAATGERHLRASLERVCACGQPDTMAVQKYDVRRP
                     PERGGGFEERWWSPVNSPVFDARGKVVQLIHRVEDVTEFVRLHELGAEREKINVQLQA
                     QAATMEADILRRASELQEVNEQLRSAHEEVSRLYERTRELDQLKTSFFANASHELRTP
                     LTLILGTVQLLLDTPGLDGQVRSRLETVVLNAQTLLRHVTDILDLSRLEAGALAPRFE
                     RTDVARLARVVAAHFETLAVEREIALNVAADEAMLADVDEALMQRVLLNLLSNAFKFS
                     PAGSAISVGVRSEEEQVVIEVADGGPGVPQGSREAVFERFRQLDGGPGRRHPGTGLGL
                     AIVREFVQLHGGSASVDDAPGGGARFTIRVPLRHPDAAVGHRHGTTSAVPDVQAMLHE
                     LLPGRQQPSPVGDDQAPLVLVVEDNADMRQFIREALAPQCRVIEAADGAQGLDKAVAE
                     RPDIVLTDIMMPGVPGNEFLSVLRADPAFDDVPVIVLSARADDAMRVELLRHGAQDYL
                     TKPFAVEELRARVGTLLAGRRAMERVRRSEESWRSLFEQSSEAILLAEADGRVLEANR
                     AAHAMLGEQVFIGRNLQELIGRGARSMAAAGMAGGVREITVRRADGSLLPVECRSQSL
                     KDGRLLCLLIDITARRESQEATRALTDELERRVAARTGQIYRLAIELESAEQRARQQI
                     ARDLYDDLGQTLIAARMFLEPLAADERADVREHVNELMLLLDQANRGVRSLGAQLAPA
                     ILDELGLGAALGWLARQIARQFGLEVAIEGDTDELPLSPEARTVSYRAVRELLINVAK
                     HARTGRATVHIERQAREMVVEVTDAGVGFDPALMRGSGGFGLSSIRERLACLGGSFDI
                     HRKPGDGMVATIRVPLELLPSSVASA"
     gene            3607..6330
                     /locus_tag="VF453_05215"
     CDS             3607..6330
                     /locus_tag="VF453_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7687080.1"
                     /translation="MFQPTKINAGLQIAFGNAALKRSIPMLTAALFGLPAVAQTSPDA
                     NTPDTTQRVLVTGSMIPRTSAETAEAITVISAQSLKDMGIQTVEQALQQIASNQATTL
                     TSSTVTTWGTGGGSFASLRGLGASRTLVLLDGQRLTPNAQVDSSADLNSIPFAAIERI
                     EVLREGASSQYGADAIAGVINFITRKDMKGGELNVTGQKSQGAGGNTYGADVTYGLGS
                     LGSDGYNLMGSLSYTKQNELRALQRGFAQRGGGDNAYYTSPASFKDANGNLFSVDYPD
                     CGKSTGVISTYLSTANGYCGYQYTNATDLIPQSSVASGMLQFSKALGSDNTLKLQYFA
                     TQSKVKTWGGAYSYAETMNPTFNAAYFPTAARSTPNTAFFNTPADATPDLTQDITVLW
                     TDPGNNRYQGDHSTEQRFLASLEGSHADWDYQANLNYSQNLSTVYLSGGYPDETLIQE
                     TDANGDAVLNHLINPFGPQTAAGQAVIDNTYQAGNLDTARLRMVDVNGSASHSVGDWF
                     HAGNATLALGIGARREQINSGTTPLAARMSAETGFYPSSVSGKRTSEAAYAELNVPVT
                     KQLEFTVSDREDHYSDFGNTNNAKASFRFQPSKLVTFRGAVSTGFRAPSLVNLFSPQI
                     LGASTNFSGSVCQQFAGICGAQGMVVTGGNPDLKPEKSDNYDLGMVLAPTTNLGMTLD
                     FYRVTISNQITTLTSSTIYKNYNTFSNLYHLNSNGSLSIAGDHSCDAGASAPTCGYIL
                     RTVQNSGGVTTNGIDVSVNYAFNTSAGRFRTSLEGNWTSQYKLQAYEGAKWQNIRGDY
                     SAGYNPVLAWQDLLTVDWSKDVWGAGLSNHYESGYKDQDGGRHVGSYSIWSGYGSWKA
                     TKQVTLLAGVRNLLNTKPSFSNQTDEWQEGYNPVLADPIGRAFYAKMTVEF"
     gene            complement(6507..7955)
                     /locus_tag="VF453_05220"
     CDS             complement(6507..7955)
                     /locus_tag="VF453_05220"
                     /inference="COORDINATES: protein motif:HMM:NF017870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HEX7687081.1"
                     /translation="MNKSALVAAGVVAAACAAYVGAAVVGGQKVEKALRAVPVAAQAQ
                     WPMVKVTEERYDRGLFTATHTVTLRLGCDAAAAGASSPQASVTIVQHVQHGPFPGFAG
                     YGAATVDTELALDKGTRQDLVKFAGTDQPLKVHTDVAFDGATHTHLTIASFRTPDAAG
                     PQVAFQGLTGDIDSDAHAIEYDLRAPGLSVAEAASAPVAMRMNFKGAHVHARAEGAGE
                     LALRAGKSQGELESAEVDFVSAPDAGGTPHKLAFEQFRFSQDTSIDKGLMNTVARADG
                     HGQVDDTKLDRIEMQATMKRFDAAAYQGVVRRMVGNDPATCGRKAPDPAQLFASQDVQ
                     AALLRMLSANPEFSLDRLAVEVGGKRAELGYLIGVNGFTADDAKLPLPASLMARGYGT
                     ARIKLPADWIQQAMTYVAQQSGQSSDIGDRAAMVELMLGKAIDGGYVVREDDMLRSEV
                     AFRNGQATINGKPLGGPAAAGAAAAGAAPTPL"
     gene            complement(8085..8777)
                     /locus_tag="VF453_05225"
     CDS             complement(8085..8777)
                     /locus_tag="VF453_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687082.1"
                     /translation="MSAARPRSSAATAFALLLPALLVLVFALHLRSLPQFLDFHLHYQ
                     PAPPDAVVRGLVAHGPQRALLHDPHVIAYLALPLFLVTALALHRRGRVRHPRASACAC
                     AITAIGTIYLGGVFGMWTAFYDGLSHVDPRDIDGATAAFAAMTAPRGAFLLTTSLAKL
                     AFTGLLLQGLVLWSPARRDRLAASCIALGSALFLAFWDLDNWMLVGSVLLLAGLVAGL
                     GAGGLPDDARSA"
     gene            8856..>9202
                     /locus_tag="VF453_05230"
     CDS             8856..>9202
                     /locus_tag="VF453_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7687083.1"
                     /translation="MPATGHAREDVRDPADRRVSRTRRSIDEAFLALLQRRGYEAVGV
                     SDIVREADVGRATFYEHYASKDDLLRAQLRQVAAATIRLRPAVSGKAGALDATRLFAH
                     VRDVPVLHRLIAGR"
BASE COUNT         1411 a         3232 c         3223 g         1336 t
ORIGIN      
        1 gcccggcgcc ggcggcctac gagccgctgc tgcagcgcgt cttcgccggc ttcgcggaca
       61 gcgcggcgcc ggtgacgcgg gcgcaggatg tcgccgaggc ggtgtggcgc gcggcgacgg
      121 atgccgcctg tccgatgttc ctgccggcag gggcggatgc ggtggccgcg acggaggccg
      181 ccggtcaatg acccgtcgcg cggggcgggc cgtcatgcgc cggccttcct cgacgcggta
      241 cgaacacggc ggtcgactcg acccgggtac gggcggacgg ccccgcgtgc gcaggactca
      301 cgcgctcgcg acggaggacg gcagcagctc caggggcacg cgaatggtgg ccaccatgcc
      361 gtcgcccggc ttgcggtgga tgtcgaagga gccgccgagg caggccagcc gctcgcggat
      421 gctggaaagg ccgaagccgc cgctcccgcg catcagcgcc gggtcgaacc cgacgccggc
      481 gtcggtcact tcgaccacca tctcgcgggc ctgccgttcg atgtgcacgg tcgctcgccc
      541 cgtgcgcgcg tgcttggcga cgttgatcag caactcgcgc accgcgcggt agctgacggt
      601 ccgggcctcg ggggacagcg gcaattcgtc ggtgtcgccc tcgatcgcca cctccagccc
      661 gaactggcgg gcgatctgcc gggccagcca gccgagggcc gcacccaggc ccagctcgtc
      721 gaggatcgcg ggggccagct gtgccccgag cgagcggacg ccccggttcg cctggtcgag
      781 caggagcatc aattcgttga cgtgctcgcg gacgtcggcg cgctcgtcgg ccgccagcgg
      841 ttcgaggaac atgcgagccg cgatgagcgt ctggccgagg tcgtcgtaca ggtcgcgcgc
      901 gatctgctgc cgggcccgct gctccgcgga ttcgagctcg atggccagcc gatagatctg
      961 cccggtgcgc gcggccacgc gccgctccag ttcgtcggtg agtgcgcggg tcgcctcctg
     1021 actctcgcgg cgcgccgtga tgtcgatcag caggcacagc aggcgcccgt ccttgagcga
     1081 ctggctgcgg cactcgaccg gcagcaggct gccgtcggcc ctgcgcaccg tgatctcgcg
     1141 gacgccgccg gccatgcccg ccgcggccat gctgcgggcg ccgcgcccga tcaattcctg
     1201 caggttgcgg ccgatgaaga cctgctcgcc gagcatggcg tgcgccgcgc gattcgcctc
     1261 cagcacgcgg ccgtcggctt cggccagcag gatcgcctcg ctcgactgct cgaacagcga
     1321 tcgccacgat tcctcgctgc gccggacgcg ttccatcgcc cgtcggcccg ccagcaaggt
     1381 gcccacgcga gcccggagct cctccaccgc gaacggcttg gtcaggtagt cctgggcacc
     1441 gtggcgcagc agctcgacgc gcatcgcatc gtcggcccgg gcgctcagca cgatgacggg
     1501 cacgtcgtcg aacgcgggat cggcgcgcag cacggacagg aactcgttgc ccggcacccc
     1561 gggcatcatg atgtcggtga gcacgatgtc ggggcgctcc gcgacggcct tgtccaggcc
     1621 ttgcgcgccg tccgccgcct cgatgacgcg gcactgcggg gccaacgcct cccggatgaa
     1681 ctggcgcatg tcggcgttgt cctcgacgac cagcaccagg ggggcctggt cgtctccgac
     1741 cggcgaaggc tgctggcggc cgggcagcag ttcgtgaagc atcgcctgca cgtcgggcac
     1801 ggcggaggtc gtcccgtgac ggtgcccgac cgccgcgtcc ggatgccgca gcggaacccg
     1861 gatcgtgaac cgcgcgcctc cgcccggcgc gtcgtcgacg ctcgcggatc ctccgtgcag
     1921 ctgcacgaac tcgcggacga tcgcgaggcc gaggccggtg ccggggtgcc ggcgaccggg
     1981 gccgccatcg agctgcctga agcgctcgaa gacagcctcg cgcgatccct gcggaacgcc
     2041 ggggccgccg tcggcgactt cgatgacgac ctgctcctcc tccgaccgga cgccgaccga
     2101 tatggcgctg ccggcaggcg agaacttgaa ggcgttggac agcaggttca gaaggacgcg
     2161 ctgcatgagc gcttcgtcga cgtcggccag catggcctcg tccgccgcga cgttcagcgc
     2221 gatctcgcgc tccaccgcga gcgtctcgaa gtgggcggcg acgacgcgcg ccagccgcgc
     2281 cacgtcggta cgctcgaatc ggggggcgag ggcgcccgcc tcgagccgcg acaggtccag
     2341 gatgtccgtg acgtggcgca gcaaggtctg cgcgttgagg acgacggtct cgagccggga
     2401 gcgcacctgg ccgtcgaggc ccggggtgtc gagcagcagc tgaaccgtgc ccaggatgag
     2461 cgtcaggggc gtgcgcagct cgtggctcgc gttggcgaag aagctggtct tgagctggtc
     2521 gagctcgcgc gtccgttcgt agagccgcga gacttcctcg tgggcgctgc gcagctgctc
     2581 gttgacctcc tgcagctcgc tcgcgcgcct gaggatgtcg gcctccatgg tcgctgcctg
     2641 cgcctgcagc tgcacgttga tcttctcgcg ttcggcgccg agttcgtgca ggcggacgaa
     2701 ctcggtgacg tcctcgacgc gatggatcaa ctgcacgacc ttcccccggg cgtcgaagac
     2761 gggggagttg accggactcc accaacgttc ctcgaatccg ccgccacgtt ccggcggtcg
     2821 tcggacgtcg tacttctgta ccgccatcgt gtcgggctgg ccgcaggcgc acacgcgttc
     2881 gagcgatgcg cgcaggtggc gctccccggt cgccgccggg tcctggggat tgtcgggaaa
     2941 gacctcgaac agcggccggc cgacgatgtc ctcgcgccgg gtcatcgtcg cctggaggta
     3001 ggcatcggtc accgcgacga tcgtgaagcg cggcccgtcc gcccggagcg ccaggtacga
     3061 gcccggcgac gcctcgaaca gcttgcgaaa gtcggcagtt ccggcgctgg cggacggcat
     3121 ggggactccc ggcggcccgg cgcgtgtgga tcgcgcggat cgagcctcgc gccactctag
     3181 gcggccgggt cgccggttcg cttgcgctgg gtcaaccggc cgaaggcgcc tcgcagtcgt
     3241 ggcaaggcga tggtgtggtc gcggtgggcg gccacggatg ccacctgtcc agtgtgttgc
     3301 tgccggcggg gcggacgcgg ggccgcgatg gcgggcacgg gagcacggtg acgccgcccc
     3361 gggatacggc cgaacgcatc tgtcgtcact gtccttgcaa cttgcctagt caatagagat
     3421 tcgcctgtgt cggcaattcc gcggctttct cgcaacggct tcaacgttct ccccagatct
     3481 gggcaacgtc gacttgagcg aggatggcct cgcgaacgtc ctgggcccat tcccttcgtg
     3541 ggctcgtaag ccgatcccgc gatgacccgg acgtcgagtt gcataccaac ttcctcagga
     3601 ggatcaatgt tccaacccac gaagatcaac gcaggtcttc aaatcgcatt cggcaacgcc
     3661 gccctcaagc gctcgatccc gatgctcacc gccgcgctgt tcggcctgcc ggcggtcgcg
     3721 cagacgagcc cggatgcgaa cacccccgac acgacgcagc gcgtcctggt tacgggcagc
     3781 atgattccgc gcaccagcgc cgagaccgcc gaggccatca ccgtcatctc cgcgcagagc
     3841 ctgaaggaca tgggcatcca gacggtggag caggcgctgc agcagatcgc ctcgaaccag
     3901 gcgacgacgc tgacctccag caccgtgacg acgtggggca cgggcggcgg ctcgttcgcc
     3961 agcctgcgcg ggctgggcgc gtcccgcacg ctggtgctgc tggacggcca gcgtctcacg
     4021 cccaatgccc aggtcgacag ctccgccgac ctcaacagca tcccgttcgc ggccatcgag
     4081 cgcatcgaag tgctgcgtga aggggcgtcg tcccagtacg gcgcggatgc gatcgccggc
     4141 gtgatcaact tcatcacgcg caaggacatg aagggcggcg agctgaacgt gaccggtcag
     4201 aagtcgcagg gcgcgggtgg caacacctac ggcgcggacg tcacctacgg cctcggcagc
     4261 ctgggctcgg acggctacaa cctgatgggc tcgctcagct acaccaagca gaacgagctg
     4321 cgcgcgctgc agcgcggctt cgcccagcgc ggcggcggcg acaacgccta ctacacgtcg
     4381 ccggccagct tcaaggacgc caacggcaac ctgttctccg tcgactatcc ggactgcggc
     4441 aagagcacgg gcgtcatctc gacctacctc agcacggcca acggctactg cggctaccag
     4501 tacaccaacg cgacggacct gatcccccag tcgagcgtgg cgtccgggat gcttcagttc
     4561 agcaaggcgc tgggcagcga caacacgctg aagctgcagt atttcgcgac gcagtcgaag
     4621 gtcaagacgt ggggtggcgc gtactcgtat gccgagacga tgaacccgac tttcaacgcg
     4681 gcgtacttcc cgacggccgc gcgctcgacg cccaacaccg cgttcttcaa cacgcccgcg
     4741 gacgccaccc cggacctgac gcaggacatc acggtgctgt ggaccgatcc tggcaacaac
     4801 cgctaccagg gcgaccactc caccgagcag cgcttccttg cttccctcga gggctcgcac
     4861 gccgactggg actaccaggc caacctcaac tacagccaga acctcagcac ggtctacctt
     4921 tctggcggct atcccgacga gaccttgatc caggaaaccg acgccaacgg cgacgcggtg
     4981 ctcaatcacc tgatcaaccc gttcggcccg cagacggccg ccggccaggc ggtgatcgac
     5041 aacacgtacc aggccggcaa cctggacacc gcgcgcctgc gcatggtgga cgtcaatggc
     5101 tcggccagcc attcggtggg cgactggttc cacgcgggca acgcgacgct ggccttgggc
     5161 atcggcgcgc gccgcgagca gatcaacagc ggcacgactc cgttggcggc gcggatgtcc
     5221 gcggagacgg gcttctaccc gtccagcgtg tcgggcaagc gcacgtccga agcggcgtac
     5281 gccgagctga acgtgcccgt gaccaagcag ctggaattca cggtgtccga tcgtgaggac
     5341 cactacagcg acttcggcaa cacgaacaac gccaaggcgt cgttccgctt ccagccgtcc
     5401 aagctggtca ccttccgagg tgccgtctcc acgggcttcc gcgcgccgtc gctcgtcaac
     5461 ctcttctcgc cgcagatcct gggcgccagc accaacttca gtggctcggt ctgccagcag
     5521 ttcgcgggca tctgcggtgc gcagggcatg gtggtgaccg gcggcaatcc ggacctgaag
     5581 cccgagaagt cggacaacta cgacctgggc atggtgctcg cgccgacgac caacctgggc
     5641 atgacgctgg acttctaccg cgtcacgatc agcaaccaga tcacgaccct gacgagctcg
     5701 acgatctaca agaactacaa cacgttcagc aacctctacc acctgaacag caacggctcg
     5761 ctgtcgatcg ccggcgacca cagctgcgat gccggtgcct cggcgccgac ctgcggctac
     5821 atcctgcgca cggtgcagaa ctcgggcggc gtgacgacca acggcatcga cgtgagcgtg
     5881 aattacgcgt tcaacacctc cgccggtcgt ttccgcacca gcctggaggg caactggacg
     5941 agccagtaca agctccaggc gtacgagggc gcgaagtggc agaacatccg tggcgactac
     6001 agcgcgggct acaacccggt gctggcctgg caggacttgc tgacggtgga ctggagcaag
     6061 gacgtctggg gtgccggcct gtcgaaccac tatgagtccg gctacaagga ccaggacggt
     6121 ggccgccatg tcggcagcta ctcgatctgg agcggctacg gctcctggaa ggcgaccaag
     6181 caagtcaccc tgctcgccgg ggtgcgcaac ctgctcaaca ccaagccgtc gttctccaat
     6241 cagaccgacg aatggcagga aggctacaac cccgtgctgg ccgacccgat cgggcgcgcg
     6301 ttctacgcga agatgacggt cgagttctga ccaccccggg gccgcgcctg agcggcggcc
     6361 cgcggcgacc tccacgggtc ggcgcctgcc tcgtggcggg ctccggcccg ttcttcatgg
     6421 gcgaaggcta tcggtggcct cgagacgcga cggatcgcaa cgtcgaccca aggggccgga
     6481 tcccgacccc gaccccggtc cgctcgtcac agcggcgtcg gcgccgcgcc tgcagccgct
     6541 gcgccggccg cggccgggcc gcccagcggc ttgccgttga tcgtcgcctg gccgttcctg
     6601 aacgccacct cggagcgcag catgtcgtcc tcgcgcacga cgtagccccc gtcgatcgcc
     6661 ttgcccagca tgagttcgac catcgcggcg cggtcgccga tgtcgctgga ctggcccgac
     6721 tgctgcgcga cgtaggtcat cgcctgctgg atccagtcgg cggggagctt gatgcgcgcg
     6781 gtgccgtagc cgcgcgccat caggctcgcc ggcagcggaa gcttcgcgtc gtcggccgtg
     6841 aagccgttga cgccgatcag gtagcccagc tcggcgcgct tgccgccgac ctcgacggcg
     6901 agcctgtcca gcgagaactc gggattggcc gacagcatcc gcagcagcgc cgcctgcacg
     6961 tcctgcgacg cgaacagctg ggccgggtcg ggcgccttcc tgccgcaggt cgccgggtcg
     7021 ttgcccacca tgcggcgcac gacgccctgg taggccgcgg cgtcgaagcg cttcatcgtc
     7081 gcctgcatct cgatcctgtc gagcttcgtg tcgtcgacct ggccgtggcc gtcggcgcgc
     7141 gcgacggtgt tcatcaggcc cttgtcgatc gaggtgtcct gcgagaacct gaactgttcg
     7201 aaggcgagct tgtgcggcgt gccgccggcg tccggcgcgg agacgaagtc cacctctgcc
     7261 gattccagct cgccctggct cttgccggca cgcagcgcga gctcgcccgc gccttcggcc
     7321 cgcgcatgca cgtgggcccc cttgaagttc atgcgcatgg ccacgggggc gctggcggcc
     7381 tcggcgacgc tcaggcccgg cgcgcgcagg tcgtactcga tcgcgtgggc gtcgctgtcg
     7441 atgtcgccgg tcaggccctg gaacgcgacc tgcgggccgg cggcgtcggg cgtgcggaag
     7501 ctcgcgatcg tgaggtgggt gtgggtcgcg ccgtcgaagg cgacgtcggt gtgcaccttg
     7561 agcggctggt cggtgccggc gaacttcacg agatcctggc gcgtgccctt gtccagcgcc
     7621 agctcggtgt cgaccgtcgc ggcgccgtag ccggcgaatc ccgggaaggg cccatgctgg
     7681 acgtgctgca cgatcgtgac ggacgcctgc ggcgacgacg ccccggccgc cgccgcgtcg
     7741 cagccgaggc gcagggtgac ggtgtgggtc gcggtgaaga ggccgcggtc gtagcgttcc
     7801 tcggtcacct tgaccatcgg ccattgcgcc tgcgcggcga ccggcacggc ccgcaacgcc
     7861 ttctcgacct tctggccgcc gacgacggcc gcgccgacat aggccgcgca cgccgcggcg
     7921 acgacgcccg cggcgacgag tgctgacttg ttcatgtgtc ctctcccctt cgatcttcga
     7981 agtcggttct tatggagtcg cggcctcacg gcggcgacga ttcgggacgc cgatgatatg
     8041 cgctcttcgg ggcgggaaat gcgggcggcg gggcgcagca gggctcaggc ggagcgcgcg
     8101 tcgtccggaa ggcctcccgc gcccaggccc gcgaccagcc cggccagcag cagcacgctg
     8161 cccacgagca tccagttgtc gagatcccag aacgcgagga acagcgcgct gcccagggcg
     8221 atgcacgacg ccgcgaggcg gtcgcgacgg gccgggctcc agaggacgag tccctgcagc
     8281 agcagcccgg tgaaggccag cttggcgagc gaggtcgtga gcaggaaggc gccgcgcggc
     8341 gcggtcatcg cggcgaaggc ggcggtggcg ccgtcgatgt cgcgcgggtc gacgtgcgag
     8401 aggccatcgt agaaggccgt ccacatgccg aagacgccgc cgaggtagat cgtgccgatg
     8461 gcggtgatcg cgcaggcgca ggcgctcgcg cgcgggtggc ggacgcggcc gcggcggtgc
     8521 agcgcgagcg cggtgacgag gaacagcggc aacgccagat acgcgatcac gtgcggatcg
     8581 tgcagcagcg cgcgctgcgg gccatgggcg acgagcccgc gcacgacggc gtcgggcggc
     8641 gcgggctggt agtggagatg gaagtccagg aactgcggca ggctgcgcag gtggagcgcg
     8701 aagacgagca ccagcagcgc cggcaggagc agggcgaacg cggtggcggc ggacgaacgg
     8761 gggcgtgcag cggacatgtc gagggcgatc ggtggtgggg acgcggctta tgatcggcgc
     8821 gccgtcccct cgaatccacc cgcagccgac cccgcatgcc cgctaccgga cacgcccgcg
     8881 aagatgtccg ggatcccgcc gaccgccggg tctcgcgcac gcgccgcagc atcgacgagg
     8941 ccttcctcgc gctgctgcag cgccgcggct acgaggccgt cggcgtcagc gacatcgtgc
     9001 gcgaggccga cgtcggccgc gcgaccttct acgagcacta cgcctccaag gacgacctgc
     9061 tgcgcgcgca gctgcgccag gtcgcggcgg cgacgatccg ccttcggccc gcggtgtccg
     9121 gcaaggccgg ggcgctggat gcgacgcggc ttttcgcgca cgtgcgcgac gtgccggtgc
     9181 tccatcgcct gatcgcgggg cg
//
LOCUS       DASVLN010000231         4571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_119001, whole genome shotgun sequence.
ACCESSION   DASVLN010000231 DASVLN010000000
VERSION     DASVLN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119001"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VF453_05235"
     CDS             complement(<1..295)
                     /locus_tag="VF453_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX7687084.1"
                     /translation="MSPTASPPLPAWPYPLWIAHRGAGRHAPENTLAAFREGFAHGFA
                     MFECDVKLSADHVPFLLHDSALERTSSGHGRAGDRPWAALSRLDAGAWLAPRWA"
     gene            580..4527
                     /locus_tag="VF453_05240"
     CDS             580..4527
                     /locus_tag="VF453_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEX7687085.1"
                     /translation="MNAPLATTLAAAATIAQAHPAAARLREIPYNYTSFSDREIVQRL
                     LGVRAWEILNRLRDERQTGRSARMLYEVLGDIWVVQRNPYLEDDLLDNPKRRQMLIDA
                     LNHRLAEVEKRRTPQADPQRDAVVAELLVGARGAVAAFGKHFSDVWDLRQRARKVLGK
                     VTARDNIKFDGLSRVSHVTDATDWRIEYPFVVLTPDTEAEMAGLVAGCVELGLTIVPR
                     GGGTGYTGGAVPLTWMSAVINTEKLEAMTEVELVDLPGHASPVATVWTEAGVVTQRVS
                     DAADRAGYVFAVDPTSAEASCIGGNIAMNAGGKKAVLWGTALDNLASWRMVTPEAKWL
                     EVVRLDHNLGKIHDAPVARFELRYFDATGKKLERTETLEIPGRQFRKEGLGKDVTDKF
                     LAGLPGIQKEGCDGLITSARWVVHRMPAHVRTVCLEFFGNPKEAVPSIVDIKDFMFAE
                     MKRPGGAVLAGLEHMDDRYLKAVGYATKSKRGGLPKMVLIGDIVGDDADAVARATSEV
                     IRLANGRSGEGFVAVSAEARKKFWLDRKRTAAIAKHTNAFKVNEDVVIPLERMGEYTL
                     GIERINIELSLRNKLALLDALERFVVDGPLPLGKTDDGGQAIATPELLGDRVQRAQAL
                     LGDVRALWTRWMHGLDVVQPADGQEPARSFFERMQDHTLRASWKTQVLAPLQAIFAGA
                     EFQPVIEECKRIHKQVLRGRTWVALHMHAGDGNVHTNIPVNSDNYEMLQTAHEAVGRI
                     MTLARSLDGVISGEHGIGITKLEFLSDDELADFASYKAKVDPQGRFNKGKLLRGESLK
                     GLGDDVRHADLSHAYTPSFGLMGHESLIMQQSDIGAISASIKDCLRCGKCKPVCATHV
                     PRANLLYSPRNKILATSLLVEAFLYEEQTRRGVSLRHWEEFEDVGDHCTVCHKCVTPC
                     PVDIDFGDVSMNMRNLLRKMGRKSFRPAAAGAMFMLNATNPQTIKIARTAMAGVGAKV
                     QRLANDVLRPAARKQTSAPPATVGPAPVREQVIHFINKKMPGGLPKKTARALLDIEDK
                     NYVPIIRDPKATTADSEAVFYFPGCGSERLFSQVGLATQAMLWHAGVQTVLPPGYLCC
                     GYPQRGSGLFDKAEEMITDNRVLFHRVANTLNYLDIKTVVVSCGTCYDQLQGYKFEEI
                     FPGCRIVDIHEFLLEKGIKLESGPGYLYHDPCHTPMKQQDPMKTVKALVGDAVVKSAR
                     CCGESGLLGLTRPDISTQVRFRKEEELRKDEAALRALQADRGLARPDADLKILTSCPS
                     CLQGLSRYGDDLQNGLLEADYIVVEMARRILGEDWMPDYVRRANAGGIERVLV"
BASE COUNT          746 a         1611 c         1607 g          607 t
ORIGIN      
        1 ccgcccagcg cggcgccagc caggcgcccg cgtccaggcg cgacagcgcc gcccagggcc
       61 ggtcgcccgc gcggccgtgg ccggagctgg tgcgctcgag ggcgctgtcg tgcagcagga
      121 aggggacatg gtcggccgag agcttgacgt cgcactcgaa catcgcgaag ccatgggcga
      181 agccctcgcg gaaggcggcg agcgtgttct cgggcgcgtg gcggccggcg ccgcggtgcg
      241 cgatccagag cggatagggc caggcgggga gcggagggga ggcggtcggg gacatcggtt
      301 gccgaacttc agccgggaag gggatgcggc gcgcgaccgg cttcgctcgc cgcgaagcgc
      361 cgtttgcccc aacgggaagc cgggtgtcga cggcgtaccg cagagtgtag gccccgcgcg
      421 ccgccgggtg cggactggac gccacacccc agaccccacg gcggcgccgg cgtgcggcgc
      481 tgggttacga ttcgactcca cgccgcggcc ttttcccgtc ttccgggcat cgcccctgcc
      541 gcgctccagg cgtggacacg caccgcccgt gccccgccca tgaacgcacc gctcgccacg
      601 accctggccg ccgccgcgac gatcgcgcag gcccatcccg ccgccgcccg gctgcgggag
      661 atcccgtaca actacacctc gttctccgac cgcgagatcg tgcagcgcct gctgggcgtg
      721 cgcgcctggg agatcctgaa ccgcctgcgc gacgagcgcc agaccggccg ttccgcgcgc
      781 atgctctacg aggtgctcgg cgacatctgg gtcgtgcagc gcaaccccta cctcgaggac
      841 gacctgctgg acaacccgaa gcgccggcag atgctcatcg acgcgctgaa ccaccgcctc
      901 gccgaggtcg agaagcgccg cacgccgcag gccgacccgc agcgcgacgc cgtggtcgcc
      961 gagctgctcg tcggcgcgcg cggcgccgtc gcggccttcg gcaagcactt cagcgacgtc
     1021 tgggacctgc gccagcgcgc gcgcaaggtg ctgggcaagg tcacggccag ggacaacatc
     1081 aagttcgacg gcctgtcgcg cgtctcgcat gtcaccgacg ccacggactg gcgcatcgag
     1141 tacccgttcg ttgtcctcac gcccgacacc gaggccgaga tggccggcct cgtcgccggc
     1201 tgcgtcgagc tcgggctgac catcgtcccg cgcggcggcg gcaccggcta caccggcggc
     1261 gccgtgccgc tgacgtggat gagcgcggtc atcaacaccg agaagctgga ggcgatgacc
     1321 gaggtcgagc tggtcgacct tcccggccac gccagccccg tcgccaccgt ctggacagag
     1381 gccggcgtcg tcacgcagcg cgtctccgac gccgccgacc gcgcgggcta cgtcttcgcg
     1441 gtcgacccga cctcggcgga ggcgagctgc atcggcggca acatcgcgat gaacgccggc
     1501 ggcaagaagg ccgtgctgtg gggcaccgcg ctggacaacc tcgcgagctg gcgcatggtc
     1561 acgcccgagg ccaagtggct ggaggtcgtg cggctggacc acaacctcgg caagatccat
     1621 gacgcgccgg tcgcccgctt cgagctgcgc tatttcgatg ccacgggcaa gaagctcgag
     1681 cgcaccgaga cgctggagat cccgggccgc cagttccgca aggaaggcct gggcaaggac
     1741 gtcaccgaca agtttctcgc cggcctgccc ggcatccaga aggaaggctg cgacggcctc
     1801 atcacgagcg cgcgctgggt cgtgcaccgc atgcccgccc acgtgcgcac cgtgtgcctg
     1861 gagttcttcg gcaacccgaa ggaggccgtg ccctcgatcg tcgacatcaa ggacttcatg
     1921 ttcgccgaga tgaagcgccc gggcggcgcc gtcctcgcgg gcctggagca catggacgac
     1981 cgctatctca aggcggtcgg ctacgcgacc aagagcaagc gcggcggcct gccgaagatg
     2041 gtgctgatcg gcgacatcgt cggcgacgac gcggacgcgg tcgcgcgcgc gacgagcgag
     2101 gtgatccgcc tggccaacgg gcgctcgggc gagggcttcg tcgcggtgag cgccgaggcg
     2161 cgcaagaagt tctggctcga ccgcaagcgc accgccgcga tcgcgaagca cacgaacgcg
     2221 ttcaaggtca acgaggacgt ggtgatcccg ctcgagcgca tgggcgagta cacgctgggc
     2281 atcgagcgca tcaacatcga gctgagcctg cgcaacaagc tggcgctgct cgacgcgctg
     2341 gagcgcttcg tcgtcgacgg cccgctgccg ctgggcaaga ccgacgatgg cggccaggcg
     2401 atcgcgacgc ccgagctgct cggcgaccgc gtgcagcgcg cgcaggcgct gctcggcgac
     2461 gtgcgggcgc tgtggacgcg ctggatgcac ggcctggacg tcgtccagcc ggcggacggc
     2521 caggagccgg cgcgcagctt cttcgagcgc atgcaggacc acacgctgcg cgcgtcgtgg
     2581 aagacgcagg tgctcgcgcc gctgcaggcg atcttcgcgg gcgccgagtt ccagccggtg
     2641 atcgaggaat gcaagcgcat ccacaagcag gtgctgcgcg gacgcacctg ggtcgcgctg
     2701 cacatgcacg ccggcgacgg caacgtgcac accaacatcc cggtcaactc cgacaactac
     2761 gagatgctgc agaccgcgca cgaggcggtc ggccgcatca tgaccctcgc gcgcagcctg
     2821 gacggcgtca tctcgggcga gcacggcatc ggcatcacca agctcgagtt cctgtcggac
     2881 gacgagctcg ccgacttcgc gtcgtacaag gccaaggtcg acccgcaggg ccgcttcaac
     2941 aagggcaagc tgctgcgcgg cgagtcgctg aaggggctcg gcgacgacgt ccgccacgcc
     3001 gacctctcgc acgcgtacac gccgtcgttc ggcctgatgg gccacgagtc gctgatcatg
     3061 cagcagtcgg acatcggcgc gatcagcgcg tcgatcaagg actgcctgcg ctgcggcaag
     3121 tgcaagccgg tgtgcgcgac gcacgtgccg cgcgccaacc tgctgtacag cccgcgcaac
     3181 aagatcctcg cgacctcgct gctggtcgag gccttcctgt acgaggagca gacccgccgc
     3241 ggcgtgtcgc tgcgccactg ggaggagttc gaggacgtcg gcgaccactg caccgtctgc
     3301 cacaagtgcg tgacgccgtg cccggtggac atcgacttcg gcgacgtctc gatgaacatg
     3361 cgcaacctgc tgcgcaagat gggccgcaag agcttccggc cggctgccgc tggcgcgatg
     3421 ttcatgctca acgcgaccaa cccgcagacg atcaagatcg cccgcaccgc gatggcaggc
     3481 gtcggcgcca aggtgcagcg cctggccaac gacgtgctgc ggcccgcggc gcgcaagcag
     3541 acctccgccc cgccggcgac ggtgggtccg gccccggtgc gcgagcaggt catccacttc
     3601 atcaacaaga agatgccggg cggcctgccc aagaagaccg cgcgcgcgct gctggacatc
     3661 gaggacaaga actacgtccc gatcatccgc gacccgaagg cgaccaccgc cgacagcgag
     3721 gccgtcttct acttcccggg ctgcggctcg gagcggctgt tctcgcaggt cggcctcgcg
     3781 acgcaggcca tgctgtggca cgcgggcgtg cagaccgtgc tgccgccggg ctacctgtgc
     3841 tgcggctacc cgcagcgcgg ctcgggcctg ttcgacaagg ccgaggagat gatcaccgac
     3901 aaccgcgtgc tgttccaccg cgtcgccaac acgctgaact acctcgacat caagacggtg
     3961 gtggtcagct gcggcacgtg ctacgaccag ctgcagggct acaagttcga ggagatcttc
     4021 ccgggctgcc gcatcgtcga catccacgag ttcctgctgg agaagggcat caagctggag
     4081 tcggggccgg ggtacctgta ccacgatccc tgccacacgc cgatgaagca gcaggatccg
     4141 atgaagacgg tcaaggcgct ggtcggcgac gcggtcgtca agagcgcgcg ctgctgcggc
     4201 gagtcgggcc tgctcgggct cacgcggccg gacatctcga cgcaggttcg cttccgcaag
     4261 gaggaggagc tgcgcaagga cgaggccgcg ctgcgcgcgt tgcaggccga tcgcgggctc
     4321 gccaggccgg acgcggacct gaagatcctc acatcctgcc cgagctgcct gcagggcctg
     4381 tcgcgctacg gcgacgactt gcagaacggc ctgctggaag cggactacat cgtcgtcgag
     4441 atggcgcgcc gcatcctcgg ggaggactgg atgcccgact acgtgcggcg cgcgaacgcc
     4501 ggcggcatcg agcgcgtgct ggtgtgatgc ggcgcgtcgc gacccggggc gtcctgctcg
     4561 ccgttctggc g
//
LOCUS       DASVLN010000232         7172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_495788, whole genome shotgun sequence.
ACCESSION   DASVLN010000232 DASVLN010000000
VERSION     DASVLN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495788"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1136
                     /locus_tag="VF453_05245"
     CDS             156..1136
                     /locus_tag="VF453_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEX7687086.1"
                     /translation="MTSLILYTPAGVLASAKPLRKAAKRLGRMGFDVEVDEACLLRHQ
                     RFAGDDDTRLETLHRVARAAPGIALATRGGYGMTRLLDRLDWKLIARSVESGTRWVGY
                     SDVTALQMGLLAHAKATSWMGPMACDDFGRDELDEVTPDCFAEAMNGALEAIGFRTEA
                     GFDGLDVRGTTWGGNLAMLCSLLGTKHWPKVRGGILLLEDVGEHPYRVERMLLQLHQA
                     GVLGSQKAVVLGAFTDWRKSPLDRGYDLKAMVRHLRGVAGVPVLTGLPFGHVPTKVTV
                     PFGVKAHLAVQGRDAFLGWDEDHAHPRHEGGDDHAGHDHAHHADHDGHGH"
     gene            1425..3626
                     /locus_tag="VF453_05250"
     CDS             1425..3626
                     /locus_tag="VF453_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7687087.1"
                     /translation="MAWLAASACALLLAGCDNSPWPSGSASENTLFTAMQEGSPRHMD
                     PTASYWSNDTTFTYQIYEPPYEYAYLKRPYVLEGRSAEDVAKPVFLDKDGKVLPEDAP
                     SDQVAESVYDVHIKHGILFQPHPCFAKDANGHYYYHAMKPGDLRGRTSPMEFQHTGTR
                     ELVADDFVYALKRHATTRITTPVFGIFSEYVIGLKDYGALVKAEDAKLRAGEDPASLD
                     RPFLDFRKWPLAGATAPDKYTLRIRIKGRYPQWSYWMAMTFMAPVPWEADAFYAQPGM
                     AAANLTLDTWPVGTGPFMQTEYFKDRLQVMQRNPNYRGEPYPCEGTPEDKAQGLLDDC
                     GKKTPFVDKIVVVAEREQVPQRAKFRQGYYDLEVFERTDKGLEYVVEKQNSEEIRKEY
                     DEKGFRLDRFDDVNSYTLGFNMLDPVIGKGKTPQDDERHRKLREAISIAIDWDEYSKI
                     FPYRAGATAMGPIPYGIFGSREKSLEGVNPVTHRIVDGKPVLRSLDEAKKLMAEAGYP
                     DGRDEKTGRPLVLNYDFYALPTPERKVEIDWVVRQFAKLNIQLEVRATDNNQYQEKER
                     KGRQQVYWTGWNADYPDAENFLVLFYGPNSKALYDGENTSNYQNPEYDRLFEQMKSLP
                     NGPEKQALIDKMVHILQVDAPWSWGYFPYASAAVQHWIYNSKPAVLIRDHGRYLRLDP
                     AERARAQAAWNRPIWWPMVALLAAFVALLLTASRMFRRRERTNARGEVVAA"
     gene            3652..4635
                     /locus_tag="VF453_05255"
     CDS             3652..4635
                     /locus_tag="VF453_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7687088.1"
                     /translation="MLPYLVRRFFYGALILLGVNLATFFLFFTVNTPDDMARLNIGGK
                     HVTQALIDKWKVERGYDKPLYWNDAKQGAERVTQTIFWERSISLFELKFGRADADSAG
                     DIGPEVARRMWVSVQLALPLFVLQVIASTAFALLLVLFRHTRLDFWGVVTCVVMLSIS
                     SLFYIIVGQFIFSRMLRIAPISGYAPGLDVVKFLVLPIVLSLLSRLGTEARLYRAMFL
                     EEIGKDYVRTARAKGLAESVVLSRHVLRNALIPIVTSAGSYLPYVFLGSLVFESFFGL
                     PGLGAYVIDAITKQDFAVVRTMVFVGSLLYIATFVLIDVVYTWVDPRVRLG"
     gene            4655..6124
                     /locus_tag="VF453_05260"
     CDS             4655..6124
                     /locus_tag="VF453_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7687089.1"
                     /translation="MFKFVILWTDVTVWALVACGIVYGVAVMRSPNLRASWRRVFCDA
                     PALCSGIVLAACLALTLADSVHYRAALKPVAGEPAGEVAYEAVAHSLLDVALSELIAS
                     REVTYSRPFDYVSLTKDSVQVNGQMVRIAPRLKFGGAHLSDPEHQWRSDLLSRGGTAL
                     LRGALLALACCAVVVFGVARGRRQGLLDTAASILRNEGEIPWRAGLWTVAVLATLVGG
                     TASIMGWYHVLGTDTTGNDVLYQALKSVRTAFVIGSLATVATLPLAVVLGIMAGYFRG
                     WVDEVIQYVYTVLSSVPNVLLIAACVLMVQVWLDKHPDAFETGVERADVKLFLLCAVL
                     GLTGWAGLCRLLRAETMKLRELDYVQAADAFGVGPTRIMLRHIFPNVAHLMLIVTVLD
                     FSSLILYEAVLSYVGVGVDPSMNSFGGMINLARKEMSLDPVVWWSFAAAFGFMLAVVL
                     AANLFADGVRDAFDPRARAFRPRLPITRRRADPDSSASA"
     gene            6153..>7172
                     /locus_tag="VF453_05265"
     CDS             6153..>7172
                     /locus_tag="VF453_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7687090.1"
                     /translation="MLQTQDLKVALDAEIGLVKALDGLTLAIARGETFALVGESGCGK
                     SMTALALMRLLPDNGRVVSGDVRIDREDVLALSEAGMRRVRGGRIGMIFQEPSTSLNP
                     VMKVGAQIVEAIETHTALRGEAARAKAIEWLRRVGIPEPERRADDYPFRMSGGQKQRV
                     MIAMTLATEPDFLVADEPTTALDVTIQAQVLELLKDLQKEQGLGMLLITHDLAVVAGM
                     AHRVALMYAGQIIEVARAEDFFAAPRHPYARALLRALPDAGRRGQPLQAIPGTVPVLT
                     QAFGGCRFAPRCDRVMAHCATTVPELEEVAGREPSGHHHAVRCLLYVAGAPATPAAPA
                     RGAAPR"
BASE COUNT         1072 a         2532 c         2467 g         1101 t
ORIGIN      
        1 gaggagggcg ccgacgccgg gcccgacacg ccctccacgc cgccgcacca cgagtcgccg
       61 cccgccgcgc acgacgacga tggaccgatc ccggtcggcg tggccatcga gatcgatcct
      121 tccccctttc ccgacgcgca aggagcgccc gcgcgatgac ctcgctgatc ctgtacaccc
      181 ccgccggcgt gctcgcctcc gccaagccgc tgcgcaaggc cgccaagcgc ctcggcagga
      241 tgggcttcga cgtcgaggtc gacgaggcct gcctgctgcg ccaccagcgc ttcgccggcg
      301 acgacgacac gcggctcgag accctccatc gcgtcgcccg cgcggcgccc ggcatcgcgc
      361 tggccacccg cggcggctac ggcatgacgc ggctgctcga tcggctggac tggaagctga
      421 tcgccaggag cgtggagtcg ggcacgcgct gggtcggcta cagcgacgtc acggcgctgc
      481 agatgggcct gctcgcgcat gcaaaggcga ccagctggat gggccccatg gcctgcgacg
      541 acttcggccg cgacgagctc gacgaggtca cgccggactg cttcgccgaa gcgatgaacg
      601 gtgcgctgga agcgatcggc tttcgcaccg aggccggctt cgacgggctg gacgtgcgag
      661 gcacgacctg gggcggcaac ctcgcgatgc tgtgctcgct gctcggcacg aagcactggc
      721 cgaaggtgcg cggcggcatc ctgctgctgg aggacgtcgg cgagcatccc taccgcgtcg
      781 agcgcatgct gctgcagctg caccaggccg gcgtgctcgg ctcgcagaag gcggtcgtgc
      841 tgggcgcgtt caccgactgg cgcaagtcgc cgctggatcg cggctacgac ctgaaggcga
      901 tggtgcgcca cctgcgcggc gtcgccggcg tgcccgtcct caccgggctg ccgttcggcc
      961 acgtgcccac caaggtgacc gtgcccttcg gcgtcaaggc gcacctggcc gtgcagggac
     1021 gcgacgcgtt cctcggctgg gacgaggatc acgcccaccc tcgccatgag ggcggcgacg
     1081 accatgcggg ccacgaccac gcccatcacg ccgatcacga cggccacggc cactgacggc
     1141 ggcgccgcgc ggcggcgatg cttgtctaga cgtgtatggc caagggtggc gccgcgggcc
     1201 ttgcggcacg cttgctgcgc tgcaacaggg gctgaaaacc cctagccaac tgccgggcag
     1261 acctccctaa aatcggggac tcgcctcctg cggtcccgtg ccacgagccg gcctcctgca
     1321 gaaggcggtt ttgttttgtc gacggccgcg cgcaccggcg ccgggccgtc gccgtcgtgt
     1381 cctggagatc cgcaatgggc ggtgtttttc gtcgttgggg ccgcgtggcc tggctggccg
     1441 cgagcgcctg cgcgctgctg ctggccggct gcgacaacag cccgtggccg agcggctccg
     1501 catcggagaa cacgctgttc accgcgatgc aggaaggctc gccgcgccac atggacccga
     1561 cggcgtccta ctggtccaac gacacgacct tcacctacca gatctacgag ccgccctacg
     1621 agtacgccta cctgaagcgg ccgtacgtgc tcgaggggcg ctcggccgag gacgtcgcca
     1681 agccggtgtt cctcgacaag gacggcaagg tgctgcccga ggacgcaccc agcgaccagg
     1741 tggccgagag cgtgtacgac gtgcacatca agcacggcat cctcttccag ccgcacccgt
     1801 gcttcgccaa ggacgcgaac gggcactact actaccacgc gatgaagccg ggcgacctgc
     1861 gcgggcgcac gtcgccgatg gagttccagc acaccggcac gcgcgaactg gtggccgacg
     1921 acttcgtcta cgcgctcaag cgccacgcga cgacgcgcat cacgacgccg gtcttcggca
     1981 tcttctcgga gtacgtgatc ggcctgaagg actacggcgc gctggtcaag gccgaggatg
     2041 cgaagctgcg cgcgggcgag gatccggcca gcctcgaccg ccccttcctc gatttccgca
     2101 agtggccgct ggccggcgcg acggcgcccg acaagtacac gctgcggatc cgcatcaagg
     2161 gccgctaccc gcagtggagc tactggatgg cgatgacctt catggcgccg gtgccgtggg
     2221 aggccgacgc gttctacgcc cagcccggga tggccgcggc caacctgacg ctcgacacct
     2281 ggcccgtggg caccggcccg ttcatgcaga ccgagtactt caaggatcgc ctgcaggtga
     2341 tgcagcgcaa cccgaactac cgcggcgagc cgtatccgtg cgagggcacg cccgaggaca
     2401 aggcccaggg cctgctggac gactgcggca agaagacgcc cttcgtcgac aagatcgtcg
     2461 tcgtcgccga gcgcgagcag gtgccccagc gcgccaagtt ccgccagggc tactacgacc
     2521 tcgaggtctt cgagcgcacc gacaaggggc tcgagtacgt ggtcgagaag cagaactccg
     2581 aggagatccg caaggagtac gacgagaagg gcttccgtct cgaccgcttc gacgacgtca
     2641 acagctacac gctcggcttc aacatgctcg acccggtgat cggcaagggc aagacgccgc
     2701 aggacgacga acgccaccgc aagctgcgcg aggcgatctc gatcgcgatc gactgggacg
     2761 agtactcgaa gatcttcccg taccgcgccg gcgcgacggc gatgggaccg attccgtacg
     2821 gcatcttcgg ctcgcgcgag aagtcgctcg agggcgtgaa ccccgtgacc catcgcatcg
     2881 tcgacggcaa gccggtgctg cgctcgctcg acgaggcgaa gaagctgatg gccgaggccg
     2941 gctaccccga cggacgcgac gagaagacgg gccggccgct ggtcctcaac tacgacttct
     3001 acgcgctgcc gacgcccgag cgcaaggtcg agatcgactg ggtcgtgcgc cagttcgcca
     3061 agctcaacat ccagctggag gtgcgcgcca ccgacaacaa ccagtaccag gagaaggagc
     3121 gcaagggccg ccagcaggtc tactggacgg gctggaacgc cgactacccc gacgccgaga
     3181 acttcctggt gctgttctac gggccgaact cgaaggcgct gtacgacggc gagaacacgt
     3241 ccaactacca gaaccccgag tacgaccgcc tgttcgagca gatgaagtcg ctgcccaacg
     3301 ggcccgagaa gcaggcgctg atcgacaaga tggtgcacat cctgcaggtc gacgcgccct
     3361 ggtcgtgggg ctacttcccg tacgcgtcgg cggcggtgca gcactggatc tacaactcca
     3421 agcccgccgt cctgatccgc gaccacggcc gctacctgcg gctcgacccc gcggaacgcg
     3481 cgcgcgcgca ggcggcatgg aaccggccga tctggtggcc gatggtggcg ctgctggccg
     3541 ccttcgtcgc gctgctgctg acggcgtcgc gcatgttccg gcggcgcgag cgcaccaacg
     3601 cgcgcggcga ggtcgtcgcg gcctgaggcc gcccacaagg ttcgcccaca gatgcttccc
     3661 tacctcgtcc gccgcttctt ctacggcgcc ctgatcctgc tgggcgtcaa cctggcgacg
     3721 ttcttcctgt tcttcacggt caacacgccc gacgacatgg cgcgcctgaa catcggcggc
     3781 aagcacgtca cccaggccct gatcgacaag tggaaggtcg agcgcggcta cgacaagccg
     3841 ctgtactgga acgacgcgaa gcagggcgcc gagcgcgtca cccagacgat cttctgggag
     3901 cgctccatct cgctgttcga gctgaagttc ggccgcgccg atgccgacag cgcgggcgac
     3961 atcggccccg aggtcgcgcg ccgcatgtgg gtgagcgtgc agctcgcgct gccgctgttc
     4021 gtgctgcagg tgatcgcgag cacggcgttc gcgctgctgc tggtgctgtt ccgccacacg
     4081 cggctcgact tctggggggt cgtgacctgc gtggtgatgc tgtcgatctc cagcctgttc
     4141 tacatcatcg tcggccagtt catcttctcg cgcatgctgc gcatcgcgcc gatctcgggc
     4201 tacgcgccgg ggctcgacgt cgtcaagttc ctcgtgctgc ccatcgtcct gtcgctgctg
     4261 tcgcgcctgg gcacggaggc gcgcctgtac cgcgcgatgt tcctcgagga gatcggcaag
     4321 gactacgtgc gcaccgcgcg cgccaagggc ctggccgagt cggtcgtgct gtcgcgccac
     4381 gtgctgcgca acgcgctgat cccgatcgtc acgagcgccg gctcgtacct gccctacgtg
     4441 ttcctcggca gcctcgtgtt cgagagcttc ttcggcctgc cgggcctggg cgcgtacgtc
     4501 atcgacgcca tcaccaagca ggacttcgcc gtcgtgcgca ccatggtctt cgtcggctcg
     4561 ctgctgtaca tcgcgacctt cgtgctgatc gacgtcgtct acacgtgggt cgacccccgg
     4621 gtgcgcctgg gctgaaggga agggcgcctc gagcatgttc aagttcgtca tcctctggac
     4681 cgacgtcacg gtgtgggcgc tggtcgcctg cgggatcgtc tacggcgtgg cggtgatgcg
     4741 ctcgcccaac ctgcgggcga gctggcgccg cgtcttctgc gacgccccgg cgctgtgctc
     4801 cggaatcgtg ctggccgcgt gcctcgcgct gaccctggcc gacagcgtgc actaccgggc
     4861 ggcgctcaag cccgtcgccg gcgagccggc gggcgaggtg gcctacgagg cggtcgcgca
     4921 ttcgctgctg gacgtggccc tgtccgagct catcgcctcg cgggaggtga cctactcgcg
     4981 gccgttcgac tacgtgagcc tcacgaagga ttccgtgcaa gtcaacggcc agatggtgcg
     5041 catcgcgcca cgcctgaagt tcggcggtgc gcacttgtcg gatcccgagc accagtggcg
     5101 cagcgacctg ctctcgcgcg gcggcacggc gctgctgcgc ggggcgctgc tggcgctcgc
     5161 gtgctgcgcg gtggtcgtct tcggggtcgc gcgcggccgc cggcagggcc tcctcgacac
     5221 cgccgccagc atcctgcgca acgagggcga gatcccctgg cgcgccgggc tgtggacggt
     5281 ggcggtgctc gcgacgctgg tcggcggaac cgcttccatc atgggctggt accacgtcct
     5341 gggcaccgac acgacgggca acgacgtgct gtaccaggcg ctcaagagcg tgcgcacggc
     5401 gttcgtgatc ggctcgctcg cgacggtggc gacgctgccg ctggcggtgg tgctgggcat
     5461 catggccggc tacttccgcg gctgggtcga cgaggtgatc cagtacgtct acacggtgct
     5521 gtcgtccgtg cccaacgtgc tgctgatcgc cgcctgcgtg ctgatggtcc aggtctggct
     5581 ggacaagcat cccgacgcgt tcgagaccgg cgtcgagcgc gccgacgtca agctgttcct
     5641 gctgtgcgcc gtgctcggcc tgaccggctg ggccggcctg tgccgcctgc tgcgcgccga
     5701 gacgatgaag ctgcgcgagc tcgactacgt gcaggccgcc gacgcgttcg gcgtcgggcc
     5761 gacgcgcatc atgctgcgcc acatcttccc caacgtcgcg cacctgatgc tgatcgtcac
     5821 ggtgctcgac ttctcctcgc tgatcctgta cgaggcggtg ctgtcgtacg tcggcgtcgg
     5881 cgtcgatccc tcgatgaaca gcttcggggg catgatcaac ctggcgcgca aggagatgag
     5941 cctcgacccg gtggtgtggt ggagcttcgc cgcggcgttc ggcttcatgc tggccgtggt
     6001 gctcgcggcc aacctgttcg ccgacggcgt gcgcgacgcc ttcgatccgc gcgcccgcgc
     6061 cttccgtccg cggctgccga tcacgcgccg ccgcgccgac cccgattcgt ccgcgtcggc
     6121 ctgacgtcgc gtcgccccag cgagttcccc gcatgctgca gacccaagac ctgaaggtgg
     6181 cgctggacgc cgagatcggc ctcgtgaagg cgctggacgg cctcacgctg gccatcgctc
     6241 gcggcgagac tttcgcgctc gtcggcgaat ccggttgcgg caagagcatg acggcgctcg
     6301 cgctgatgcg cctgctcccg gacaacgggc gcgtggtctc gggcgacgtg cgcatcgacc
     6361 gcgaggacgt gctggcgctg tccgaggccg gcatgcggcg cgtgcgcggc ggccgcatcg
     6421 gcatgatctt ccaggagccc tcgaccagcc tgaacccggt gatgaaggtc ggcgcccaga
     6481 tcgtcgaggc gatcgagacg cacaccgcgc tgcgcggcga ggcggcgcgc gcgaaggcga
     6541 tcgagtggct gcgccgtgtc ggcatccccg agccggagcg ccgcgccgac gactatccgt
     6601 tccgcatgag cggcggccag aagcagcgcg tgatgatcgc gatgacgctg gccaccgagc
     6661 ccgacttcct cgtcgccgac gagccgacca ccgcgctgga cgtcacgatc caggcgcagg
     6721 tgctggagct gctgaaggac ctgcagaagg agcagggcct gggcatgctg ctgatcacgc
     6781 acgacctcgc ggtcgtcgcc ggcatggcgc accgcgtggc gctgatgtat gccgggcaga
     6841 tcatcgaggt cgcgcgcgcc gaggacttct tcgccgcgcc gcgccacccc tacgcgcggg
     6901 cgttgctgcg cgcgctgccc gacgccgggc gccgaggcca gccgctgcag gcgatccccg
     6961 gcacggtgcc tgtgctgacg caggccttcg gcggctgccg cttcgcgccg cgttgcgacc
     7021 gcgtgatggc gcactgcgcg acgacggtgc cggagctcga ggaggtcgcc ggccgcgaac
     7081 cctcgggtca ccaccacgcc gtgcgctgcc tgctgtacgt tgccggtgca ccggcaacgc
     7141 cggccgcacc ggcaaggggc gcggcgcccc gt
//
LOCUS       DASVLN010000233         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_495818, whole genome shotgun sequence.
ACCESSION   DASVLN010000233 DASVLN010000000
VERSION     DASVLN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495818"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1278
                     /locus_tag="VF453_05270"
     CDS             145..1278
                     /locus_tag="VF453_05270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7687091.1"
                     /translation="MRILHLDPDDLDNPLAGGGPVRTFEICRRLARRHEVTVLTPTFP
                     GSTPELVRDGVRYVRLGRRVREHGSSHHITFALALPGAVRRFGHDLLVEDFMPPASAT
                     WTPLFRRRDRPLIASVQWFFARRYTEWLKLPFHWGEEYGVRLYDHFVVLTEAMRGRIL
                     ARSPGADCRVIPNGVDEALFEVPPVDAARGRGVLFLGRTEMHAKGLDLLLQALARLPE
                     ASRPPLAIAGTVQDGAALEALIDRLALRPWVAVTGTYDPARRLELLRDCRVLAMPSRD
                     ETFGMTIAEANAAARVAVVWDRAPMNEVAAPGCPRVAPYDVDAYAAALAATLALPDDA
                     LRARGDEARAWARQYDWDAVAAAQEAFYLDVVERFRHARGIAR"
     gene            1306..1935
                     /locus_tag="VF453_05275"
     CDS             1306..1935
                     /locus_tag="VF453_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687092.1"
                     /translation="MTDTAAPPPTSIPPAVPADARPSWAQTRALIRSDLSRIAAHMEE
                     GASFAHRVYFFLLPGMQALFWYRLSRHWYLRGWTRLARFVNLFAMYLTRAEIPPTTSI
                     GPSALVAHATGVALFGRIGARFTIQGDGGCGGGFGTEDIGGGPGYPVIGDDVVLAHGA
                     KVLGAVRVGDGVHVGPCALVTTDVPAGALVLWPRPRILAGGAAPPAVKP"
     gene            1949..>2774
                     /locus_tag="VF453_05280"
     CDS             1949..>2774
                     /locus_tag="VF453_05280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7687093.1"
                     /translation="MTIDLSIIVVNWNVRDLLRECLRSTLEDGGIPPGRLELVVVDND
                     SRDGSVEMVRAEFPQLTLIANRENVGFGRANNQALPLCRGRHVLLLNPDTRVLPGALA
                     ALVRHMDETPDAAVMGCRLLNADGSLQRWTGGAYPRLLNVVNHYFFVDRLLPPGWRPL
                     PLYLDRDVAHDIDVDWVSGAAMILRADRLGGRLFDPHYFMYGEDMELCHRLKAAGGRV
                     VYTPVASIVHYQGESMKQQDADVMLSSLKGPRQFYKHVRGDRGVLAYDLATVAGFGL"
BASE COUNT          325 a         1071 c          991 g          387 t
ORIGIN      
        1 cggcgcgctc gcggtcgggc cgctggcggc gctcgcggca tcgctgggcg cctacctgct
       61 cgtgctggtc gcgagcggcg cgatccggcc gtcggcgatc gcgagcgcgc gccggcgcct
      121 cgcggccctg ttcgagaggt gagcatgcgc atcctgcacc tcgacccgga cgacctggac
      181 aacccgctgg ccggcggcgg gccggtgcgc acgttcgaga tctgccgccg cctcgcgcgc
      241 cggcacgagg tcaccgtgct cacgccgacc ttcccgggct cgacgcccga gctggtgcgc
      301 gacggggtgc gctacgtgcg cctggggcgg cgggtgcgcg agcacgggtc gtcgcaccac
      361 atcaccttcg cgctcgcgct gccgggcgcg gtgcggcgct tcgggcacga cctgctcgtc
      421 gaggacttca tgccgcccgc atccgccacc tggacgccgc tgttccgccg ccgcgaccgg
      481 ccgctgatcg cgtcggtgca gtggttcttc gcgcgcaggt acaccgagtg gctgaagctg
      541 ccgttccact ggggcgagga gtacggcgtg cggctctacg accacttcgt cgtgctgacc
      601 gaggcgatgc gcgggcgcat cctcgcacgt tcgcccggcg ccgactgccg cgtgattccc
      661 aacggcgtcg acgaagcgct gttcgaggtc ccgccggtgg acgcggcgcg cggccgcggc
      721 gtgctgttcc tcggccgcac cgagatgcac gccaagggcc tcgacctgct gctgcaggcg
      781 ctcgcgcgcc tgcccgaggc gtcgcggccg ccgctggcga tcgccggtac cgtgcaggac
      841 ggcgcggcgc tcgaggcgct gatcgaccgg ctggccctgc gcccctgggt cgcggtcacc
      901 gggacctacg accccgcgcg ccgcctcgag ctgctgcgcg actgccgcgt cctcgccatg
      961 ccctcgcgcg acgagacctt cggcatgacg atcgcggagg ccaacgccgc ggcgcgggtg
     1021 gccgtggtgt gggatcgcgc gccgatgaac gaggtggccg cgcccggctg cccgcgcgtc
     1081 gcgccctacg acgtcgacgc ctacgccgcg gcgctcgcgg cgacgctcgc gctgcccgac
     1141 gacgcgctgc gcgcccgcgg cgacgaggcc cgggcctggg cgcgccagta cgactgggac
     1201 gcggtcgccg cggcccagga ggccttctac ctcgacgtcg tcgagcgctt ccgccacgcc
     1261 cgcgggatcg cccgctgacg gcaacgcgca acgaacgaga acgacatgac cgacaccgcc
     1321 gcaccgccgc cgacgtcgat accgcctgcg gtacccgcgg acgcgcgccc cagctgggcg
     1381 cagacgcgcg ccctgatccg ctccgacctc tcgcgcatcg ccgcgcacat ggaggagggc
     1441 gcctcgttcg cccatcgcgt ctacttcttc ctgctgccgg gcatgcaggc gctgttctgg
     1501 taccggctct cgcgccactg gtacctgcgc ggctggacgc ggctcgcgcg cttcgtcaac
     1561 ctgttcgcga tgtacctgac gcgcgccgag atcccgccca cgacctcgat cggcccgtcg
     1621 gcgctggtcg cgcatgcgac cggcgtcgcg ctgttcgggc gcatcggcgc gcgcttcacg
     1681 atccagggcg acggcggctg cggcggcggc ttcggcaccg aggacatcgg cggcggaccg
     1741 ggctacccgg tgatcggcga cgacgtcgtg ctcgcccacg gcgccaaggt gctgggcgcg
     1801 gtgcgcgtgg gcgacggcgt gcatgtcggg ccgtgcgcgc tcgtcaccac cgacgtgccg
     1861 gccggcgccc tcgtgctgtg gccgcggccg cgcatcctcg ccgggggcgc cgcgccgccc
     1921 gcggtgaagc cgtgagggcg ccccggccat gaccatcgac ctcagcatca tcgtcgtcaa
     1981 ctggaacgtg cgcgacctgc tgcgcgagtg cctgcgctcg acgctggagg acggcggcat
     2041 cccgccgggg cggctcgagc tcgtcgtcgt cgacaacgac tcgcgcgacg gcagcgtcga
     2101 gatggtgcgc gcggagttcc cgcagctgac gctgatcgcc aaccgcgaga acgtcggctt
     2161 cggccgcgcc aacaaccagg cgctgccgct gtgccgcggc cgccacgtgc tgctgctcaa
     2221 cccggacacg cgcgtcctgc ccggcgcgct cgccgcgctg gtgcgccaca tggacgagac
     2281 ccccgacgcc gccgtgatgg gctgccggct gctcaacgcc gacggcagcc tgcagcgctg
     2341 gacgggcggc gcctatccgc gcctgctcaa cgtcgtcaac cactacttct tcgtcgaccg
     2401 cctcctgccg cccggctggc gcccgctgcc gctgtacctc gaccgcgacg tcgcgcacga
     2461 catcgacgtc gactgggtct cgggcgcggc gatgatcctg cgcgccgacc gtctcggcgg
     2521 ccgcctgttc gacccgcact acttcatgta cggcgaggac atggaactgt gccaccggct
     2581 gaaggccgcg ggcgggcgcg tcgtctacac gccggtcgcg tccatcgtcc actaccaggg
     2641 cgagagcatg aagcagcagg acgccgacgt catgctgtcc tcgctcaagg gcccgcggca
     2701 gttctacaag cacgtgcgcg gcgaccgcgg ggtgctggcc tacgacctcg cgaccgtggc
     2761 cggcttcggc ctgc
//
LOCUS       DASVLN010000234         7585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_441991, whole genome shotgun sequence.
ACCESSION   DASVLN010000234 DASVLN010000000
VERSION     DASVLN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441991"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VF453_05285"
     CDS             complement(<1..347)
                     /locus_tag="VF453_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7687094.1"
                     /translation="MTMIRGADGTSLFVRDWGQGRPLVFLAGWTLSSDAWAYQMQPLS
                     RQGLRCVAIDRRAHGRSDDPGRGLDFDTLADDVAAVLEALDLRDVCLVAHSFASGEAM
                     RLLTRHGGRRGRVG"
     gene            complement(442..810)
                     /locus_tag="VF453_05290"
     CDS             complement(442..810)
                     /locus_tag="VF453_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7687095.1"
                     /translation="MPARPPRRLVALLAAAALAAPALAASGRLGFAIEAETDGLFSTT
                     LKRLTITSVVDGAPAQRAGLRAQDEVEAVNGVAVVGAPGGRIRDLVAAVRAGDHLLLK
                     VRRADGEHAVDVVAGERPAD"
     gene            complement(1005..1427)
                     /locus_tag="VF453_05295"
     CDS             complement(1005..1427)
                     /locus_tag="VF453_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687096.1"
                     /translation="MLEFNIDIQKQLISAALDDFTPNSAFPNLHGGQFGDTPDAWREA
                     VVTFIQDMVAAGLIAPLAGREGYQAKSAQQIAELLRNGDPETGLDSDLLWDVMHFAGT
                     AKLADLLHALSLDDWQARSAGPCVDLARRLGEIGAIRL"
     gene            complement(1438..1704)
                     /locus_tag="VF453_05300"
     CDS             complement(1438..1704)
                     /locus_tag="VF453_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687097.1"
                     /translation="MPPSADPNATAEAFARAAFNGQTPDKLVPILSGDSWVAKLPDGT
                     AVTFRVAGDASAATDAGTATVEVNNAAVKAINDGNVAKFKFPGL"
     gene            complement(1757..2572)
                     /locus_tag="VF453_05305"
     CDS             complement(1757..2572)
                     /locus_tag="VF453_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687098.1"
                     /translation="MSSRPPGVLGTSTYPGDPHDRVLTGAGSFRSPAPAGHQAATSLQ
                     DIEHRLTELAHGLSTAVGWHQKQGAAYKLRGQHSFTAGVLWGMSENLAETVISAVDLV
                     ETLALAEYWESKQEHSLIDQVRANMFFSAIPGVRLSMTVTSHFWPGFDKRAQEAYEER
                     GAIIDAVEHAFTHPKDFFGSVTKVQEERIGQFVAFKNERSLSGNFKAGKLLGELLFDL
                     LMVIDLAVGLAKLALAVPRLARYTEDLAKLAREFAFSARPAPAAACGGAAVHP"
     gene            2861..3358
                     /locus_tag="VF453_05310"
     CDS             2861..3358
                     /locus_tag="VF453_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687099.1"
                     /translation="MNTHVTGNRLSVDIPADDLQAIRDAVQTLQDKLMPHLANIGPDE
                     KRALTKMGSKSVDFVSKGLGYTIANPQFKPAFVDIDEFSKSVAAYGLLRSLHQPVSQL
                     ADMLDDSMALAGSDAMTSALAYYQSVKNADKLNLPGAATIAADLAERFAGQGPRASSK
                     APAAA"
     gene            3430..3750
                     /locus_tag="VF453_05315"
     CDS             3430..3750
                     /locus_tag="VF453_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687100.1"
                     /translation="MPRRLAPKPRQIPAPIQRFDAGHNQFHAEWKSFHGGWNEWAASM
                     NQFDPAMNDFDAESNGFIPKIIEFIAGWNSLDARVNSLHAAMIEFTVLVRQIDAAMNE
                     FNGG"
     gene            4054..4446
                     /locus_tag="VF453_05320"
     CDS             4054..4446
                     /locus_tag="VF453_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687101.1"
                     /translation="MQIDEKAMRQMAAIRRGVAEVGYVTAVLQERGDSLAIRLDEAVL
                     RREVVATLEACDRLGLQSDADRLSLCLLEITTFAGMRDLRKLPGLLDYAGGAPEARMP
                     ALLQAMPPRVWAQITAQAPEVRRLRGIE"
     gene            4443..5303
                     /locus_tag="VF453_05325"
     CDS             4443..5303
                     /locus_tag="VF453_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687102.1"
                     /translation="MTAPESGCVPAMKPMAMAASLAQSGGLAAAKGGPMKLIQSCPCN
                     PDGRPPLAARRAIDDKLFCAVMCCCNAGASKGAADQRLMQQCVNDVFTAADKDLNWKS
                     RYKSEISYVMDPPNTPGQPMPLMSRTTGIATKPTEYWQGGARAQLGDTWVAGLGMVRR
                     PDIVVVNDPCQPPVGSNIERVVEMKFEGDTSDTEQNEAYRQIAGGADKYSVLRAGGKP
                     QPEEQPPCDCNSDENRRHVPVLTPEAQAEQERKAKMIKTAAGAGAVAAGGMAVSEILE
                     AVLVGLLAAL"
     gene            5328..6317
                     /locus_tag="VF453_05330"
     CDS             5328..6317
                     /locus_tag="VF453_05330"
                     /inference="COORDINATES: protein motif:HMM:NF023304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI immunity family protein"
                     /protein_id="HEX7687103.1"
                     /translation="MADVDTVSWPWPEEGKPWDGLPMARLGLRAALFFGDGYDPALRA
                     ALMTIIEQYVAFSGGRIRAYQRSGDRRRMTADASSPVDLEPLRGKVDEMGAPFSIEMS
                     AEADPAVASHWSMVTVANNAGYLLLHMPLSVFEGAPPHSFRNLFQKWCSELNVLHAYA
                     GLGLVLPVGGRALGGALRRSGSILTRFVGLDADDPGSTAMWCRGGIRTVNWLTAINAD
                     LLAKVGGEATVLRVAGADVQAMPYARGSIFVAGTSAQLGDMQVDNFPAAFRTLGRAVA
                     PLRADIPNAWLDAPPGYAAPPGFTSKSGWGDAEPEELPSLHYTKTWMARFDGA"
     gene            6572..6645
                     /locus_tag="VF453_05335"
     tRNA            6572..6645
                     /locus_tag="VF453_05335"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6604..6606,aa:Gly,seq:ccc)
     gene            6809..6949
                     /locus_tag="VF453_05340"
     CDS             6809..6949
                     /locus_tag="VF453_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687104.1"
                     /translation="MLAGSRARGIVAQAKVVLHSRLDQVMVVARQARPSMPARPPVQA
                     RP"
     gene            7051..7470
                     /locus_tag="VF453_05345"
     CDS             7051..7470
                     /locus_tag="VF453_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687105.1"
                     /translation="MPVPAHHVPFEAIAQRLLQARPTASDLSQALGGPAIAPGSDATF
                     PLNAAPFATVNVSEFRGEVDLVFDLATGPWTLRDVVAEPEAWSSGPRLPHSGVLESTR
                     TWTAPGRSITCVVRLDGEGPLAGRSVIGQARCQVSPV"
BASE COUNT         1236 a         2519 c         2616 g         1214 t
ORIGIN      
        1 ccgacccgcc cgcggcggcc gccgtggcgt gtcagcaggc gcatcgcctc gccgctcgcg
       61 aacgaatggg cgaccaggca gacgtcgcgc aggtccagcg cctcgagcac ggcggcgacg
      121 tcgtcggcca gcgtgtcgaa gtccaggccg cggcccgggt cgtccgagcg gccatgcgcc
      181 cggcggtcga tcgcgacgca gcgcaagccc tggcgcgaca gcggctgcat ctggtaggcc
      241 caggcgtcgc tgctgagcgt ccagcccgcg aggaacacga ggggccgtcc ctggccccag
      301 tcgcggacga agagggaggt gccgtcggcg ccgcggatca tggtcatggt gtcgctcctg
      361 gtgaggtcgt tgtcgatggc gccagtgtcg gcagccaggg gaagcgcgtc gattacctgc
      421 ggcgtcatgg cgccgcgccg gtcagtcggc gggacgctcg cccgcgacga cgtcgaccgc
      481 gtgctccccg tcggcgcgcc gcaccttcag cagcaggtgg tcgccggccc gtaccgcggc
      541 gacgaggtcg cggatgcggc cgcccggcgc gccgacgacg gccacgccgt tcaccgcctc
      601 gacctcgtcc tgcgctctca agcccgcccg ctgcgccggc gccccatcga cgaccgaggt
      661 gatggtcagc cgcttgagcg tggtcgagaa caggccgtcg gtctcggcct cgatggcgaa
      721 gcccaggcgg ccggacgcgg cgagggcggg agcggcgagc gcggcggcgg ccaggagggc
      781 gacgaggcgg cggggcgggc gtgcgggcat ggcgggcgcg ggggaagtgc aggaggcgcg
      841 atgttgcacg gcgagggggc gcgtttccac gcgcttgcga cgcactgcgt gggcgcccga
      901 cggacggctg gccgcggccg ggcgaatccc tcgggctgcg cgagttccgg cgaccgcact
      961 ccttcactag cctgagctca cgcaggcttg tgccatcccg cctttcagag acggatcgcc
     1021 ccgatctccc cgagccgacg cgcgagatcg acgcaggggc ctgcgctccg ggcctgccag
     1081 tcatccagcg acagcgcatg tagcaggtcg gcgagctttg cggtgcctgc gaagtgcatg
     1141 acgtcccaca gcagatcgga atcgagaccc gtctcgggat cgccgttgcg caggagctcg
     1201 gcaatctgtt gggcgctctt tgcctgatag ccctctcgcc ccgcaagcgg cgcaatgagt
     1261 cccgcggcga ccatgtcctg tatgaacgtg acgacggcct cgcgccatgc gtcgggcgtg
     1321 tcgccgaatt ggccgccgtg caggttcgga aaggccgaat tcggagtgaa gtcgtcgagc
     1381 gcggccgaga tcagctgctt ctggatatcg atgttgaatt cgagcatcag gcaatcctca
     1441 caagccgggg aatttgaact tcgcgacgtt cccgtcgttg atcgccttga ccgcggcgtt
     1501 gttgacttcg accgtcgccg taccagcatc tgtggccgcg gacgcatccc cggccacccg
     1561 gaaggtgacc gccgtgccgt cgggcagttt agccacccag gaatcaccgc tcaggatggg
     1621 caccagcttg tccggcgtct gcccgttgaa cgcggcgcgg gcgaacgctt cggcggtggc
     1681 gttgggatcg gcggatggag gcatgggtat cggtgttccc ggccccccga tccctgccgg
     1741 aagcttgcct tggtcgtcaa ggatggacgg cggcaccacc gcaggcggcc gctggggcgg
     1801 gtctagcgga gaaggcgaat tcgcgcgcca gcttcgccag gtcttcggtg tagcgcgcca
     1861 gtctcggaac cgccaaggcg agcttggcca ggccgacggc caagtcgatg accatcaaca
     1921 ggtcgaacag cagttccccc agcagcttgc cggccttgaa attgcccgat agcgaccgtt
     1981 cgttcttgaa ggcgacgaac tggccgatgc gctcttcctg gaccttggtc acagatccga
     2041 agaagtcctt cggatgcgtg aaggcatgct cgacggcgtc gatgatggcg ccgcgctcct
     2101 cgtacgcctc ctgcgccctc ttgtcgaagc cgggccagaa gtggctggtg accgtcatgc
     2161 ttagtcggac gccagggatg gccgagaaga acatgttggc gcggacttgg tctatgaggc
     2221 tgtgctcctg cttcgactcc caatattcgg cgagggccag cgtctccacc aggtcgaccg
     2281 ccgagatgac cgtctcggcg aggttttcgc tcatgcccca caacacgccc gcagtgaagc
     2341 tgtgctggcc acgcagcttg taggccgcgc cttgtttctg gtgccaaccg acggccgtcg
     2401 acaagccatg ggccagttcc gtcagccgat gctcgatgtc ttggagggac gtggcggcct
     2461 gatggccggc gggggcgggc gaccggaagc tcccggcgcc cgtgagcacg cgatcgtgcg
     2521 ggtcgccggg atacgtggaa gtgcccaata cgccgggagg tctgctgctc atgttgggtg
     2581 gcgaacgtca ggacggcgca cgccagcagc gcagggcgtc cgcttgcatc cctacatgat
     2641 ggggtgtcgc gcggaggctg cccgacgagg tggcgccgtg cccgcgatgc ccgttgaccg
     2701 acacgtcacg cccgctcgcc cggcaagtta gggatccggg ccgctgtggc aagagcagtc
     2761 cctagtttgc gtattgagat acccgtggcg gtatctccac aatcgcgcac ctccgcggca
     2821 aagggtcgcg gcggacgcaa ccacacacag ggagattgca atgaacaccc acgtgaccgg
     2881 gaaccgactc tcggtcgaca ttcccgccga cgacctgcag gcgatccgcg acgccgtgca
     2941 gacgctgcag gacaagctga tgccgcacct ggccaacatc ggccccgacg agaagcgcgc
     3001 cctgacgaag atgggcagca agagcgtcga cttcgtctcg aaggggctgg gctacaccat
     3061 cgccaacccg cagttcaagc ccgccttcgt cgacatcgac gaattctcga agagcgtggc
     3121 ggcctacggc ctgctgcgct cgctgcacca gccggtcagc cagctcgcgg acatgcttga
     3181 cgacagcatg gcgctcgccg gcagcgacgc gatgacctcg gcgctggcct actaccagtc
     3241 ggtcaagaac gccgacaagc tgaacctgcc cggcgcggcg accatcgccg ccgacctcgc
     3301 cgaacgcttc gccggccagg gcccgcgtgc ctccagcaag gcgcccgcgg cggcctgaac
     3361 gccccgccaa cccgccgccg acgcgccccg cgccggcggc cgaggcaccg acccgcggga
     3421 cgcgtcccgg tgcctcgcag gcttgccccg aagcctcgcc agatccccgc tccgatccaa
     3481 cgattcgacg ccggacataa tcaattccac gccgaatgga aatcattcca cggcggatgg
     3541 aatgaatggg ccgcttcgat gaatcaattc gatccggcga tgaatgattt cgacgcggag
     3601 agcaatggat tcatcccgaa gatcattgaa ttcatcgccg gatggaattc attggatgcg
     3661 cgggtgaatt cattgcatgc ggcgatgatt gaattcaccg ttttggtgcg tcagatcgac
     3721 gcggcgatga atgaattcaa tgggggatag aattcgcggc ctcgcggcct cgcggcctcg
     3781 cggcctcgcg gcgcgtcact cgcggggcag cggtcgcacg tgagggccaa gcgaaaggcc
     3841 ccccagctgg gcacgggccg gccgggcaac aagatcagct ccgcggccgc tgcgacgtgg
     3901 cctcgcaatt ccattgacct gcgcaggccc ccgcccccac tctcgagcaa ctgacgcacc
     3961 ctagtcgcct tgtttcaagc ggttgcacat gggtgcttag tgaccgggta cgaggatggg
     4021 tatattccct gcccgttcag ggagaagctg cacatgcaaa tcgatgaaaa ggcgatgcgg
     4081 cagatggcgg cgatcaggcg cggcgtggcc gaggtcggat acgtcactgc tgtcctgcaa
     4141 gaacgggggg actcgctggc gatacggctc gatgaagccg ttctcaggcg cgaggtcgtc
     4201 gcaactctcg aagcctgcga caggctggga ttgcaaagcg acgccgatcg actgtcgctt
     4261 tgcttgctcg agatcacaac ttttgccggc atgcgcgacc tgcgcaagct gcccggactc
     4321 ctcgactacg ccggcggcgc gccggaagcg cgcatgcccg ccttgctcca ggcgatgccg
     4381 ccgcgggtct gggcgcagat cacggcccag gcacccgagg tgcgtcggct gcgaggcatc
     4441 gaatgacggc tccggagagc ggctgcgtcc cggcgatgaa gccgatggcg atggcggctt
     4501 cgctggccca gtccggtggc ctggctgcgg caaagggcgg gccgatgaaa ctgattcaaa
     4561 gctgcccctg caatccggac ggccggccgc cgctggcggc caggcgggca atcgacgaca
     4621 agctgttctg tgccgtcatg tgctgctgca acgcgggcgc ctcgaagggc gctgccgatc
     4681 aacgcttgat gcagcagtgc gtcaacgacg tcttcaccgc agccgacaag gatctgaact
     4741 ggaagagccg ctacaagtcg gagatctcgt acgtgatgga tcctccgaac acgccaggcc
     4801 agccgatgcc gctcatgtcc cgtacgacgg gcattgcgac gaagcccacg gaatactggc
     4861 aaggcggcgc gcgtgctcag cttggagata cttgggtggc aggcttgggc atggttcgac
     4921 ggcccgacat tgttgttgtg aacgacccat gccagccgcc ggtcggcagc aacatcgagc
     4981 gtgtcgtcga gatgaagttc gaaggcgaca ccagcgatac ggagcagaac gaggcctatc
     5041 gccagatcgc cggaggcgcc gataagtatt ccgtgctccg cgcaggaggc aagccccagc
     5101 cggaagagca gccgccttgc gactgcaaca gcgatgagaa tcgtcgccat gtccccgtac
     5161 tgacgccgga agcccaggcc gagcaggagc gcaaggcgaa gatgatcaag accgcggcag
     5221 gcgcgggtgc ggtggcggcg ggcggaatgg ccgtctccga aattctggag gcggttctcg
     5281 taggtttgct cgcagcgctt tgagaaagac ctttcatgac aatcaccatg gccgacgtcg
     5341 acacggtttc ctggccctgg cccgaagaag gcaagccatg ggacgggctg ccgatggctc
     5401 gcctcggcct gcgtgcggcc ctcttcttcg gcgacggcta cgacccagcg ttgcgcgccg
     5461 cgttgatgac catcatcgaa cagtacgtgg cattcagcgg cggtcgcata cgcgcctacc
     5521 agcgctcggg cgatcgacgc cggatgacgg cagacgcgag ttcgcctgtc gaccttgagc
     5581 cgcttcgcgg caaggtggac gagatggggg cgccgttctc catcgagatg tccgcggagg
     5641 ccgatccggc ggtcgccagc cattggtcga tggtgaccgt ggcgaacaac gcgggctatc
     5701 tgctgttgca catgcccctg agcgtgttcg aaggcgcgcc gccgcacagt ttcaggaacc
     5761 tgttccagaa gtggtgctcg gagctgaacg tgcttcatgc ctacgcaggg ctcggattgg
     5821 tgctgccggt cggcggacgt gccctcgggg gagcgctccg acgctcaggc tccatcctca
     5881 ctcgcttcgt ggggctcgac gccgacgatc cgggctcgac ggcgatgtgg tgccgcggcg
     5941 gcatccgcac ggtcaactgg ttgacggcga tcaacgcgga cctgctggcg aaggtcggcg
     6001 gcgaagcgac cgtgctgcgg gtggccgggg cagacgtcca agcgatgccc tatgcacgcg
     6061 gatcgatctt tgtggcgggc acctccgcgc agctcggcga catgcaggtc gacaacttcc
     6121 ctgcggcatt ccggacgctc ggacgggcag tggccccact gagggcagat attccgaatg
     6181 cgtggcttga cgcgccgccc ggctatgccg caccgcctgg gttcacttcg aagtccggat
     6241 ggggcgatgc cgagcccgag gaactgccgt cgctgcacta cacgaagacc tggatggccc
     6301 gattcgacgg ggcctagcgg tgactggcag ccagctttcg ccagctggtc gtcggctccg
     6361 acactgcctc gaagctgagc acgcaggatc gagcacctcc tgaccgcgcg ctccgactga
     6421 agcgtgcggt tcggggccgg cctcgaggca tgcctcgacg gcggtatgca gttggaagat
     6481 gacgcgcgat gcgagacggg gatcgcgcac atcgcaggga cgccgcgcgg cgccgatgcc
     6541 caccgaaaat cgcgtaaact cgccccctgc cgcgggtgta gttcaatggt agaacggcag
     6601 cttcccaagc ttcatacgag ggttcgattc ccttcacccg ctccactgcc gcccaggtga
     6661 gtcctcgggc ggccggcata gagggccgtt ggcggtccgg tgcgagaagg atttcgtcgc
     6721 gaatcgcctg ggatcggcac gtttccagtg tgtcgcgtgc gtccgtcgaa tgggcctcgt
     6781 ctcgattgct cccggatggg cctcctgcat gctggctggt tcgcgcgcgc gaggcatcgt
     6841 cgcgcaggcg aaggtcgtgc tccactcccg gctcgaccag gtgatggtcg tcgcgcgcca
     6901 ggcgcggcca tccatgccgg cgaggccacc cgtgcaggcg aggccctgat gcgccgcctc
     6961 ctgcgcctcg cgacgctgtg catcgcgtcc tcctgcgccg cctgccaggg tgcgcgaccc
     7021 cccctcgatc cttccccgaa agagaccacc atgccggttc ccgcccacca cgtgcccttc
     7081 gaggcgatcg cccagcggct cctgcaggcg cggccgaccg cgtcggatct ttcgcaggcg
     7141 ctgggcggac ccgccatcgc gccgggaagc gacgccacgt tcccgttgaa cgccgcgccg
     7201 ttcgcgaccg tcaacgtcag cgagttccgc ggcgaggtcg acctggtctt cgacctcgcc
     7261 accggcccct ggacgctgcg cgacgtcgtc gcggagcccg aagcctggag ctcggggccg
     7321 cgtcttccgc acagcggcgt gctggagtcg acgcgcacct ggacggcgcc cggtcgcagc
     7381 atcacgtgcg tggtgcgcct cgacggcgag ggcccgctgg ccgggcggtc ggtcatcggc
     7441 caggcgcggt gccaggtgtc gccggtgtga tcgtcggggg ccgcggccgg cggtcctgcg
     7501 ggcgggccac cgatccttcc cggaagggga tgctgcagtc cgatttcgac tcgtcccacg
     7561 tcgagggcga ctgcggacgt gccgg
//
LOCUS       DASVLN010000235         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_441998, whole genome shotgun sequence.
ACCESSION   DASVLN010000235 DASVLN010000000
VERSION     DASVLN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441998"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VF453_05350"
     CDS             <1..516
                     /locus_tag="VF453_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEX7687106.1"
                     /translation="SDAIARATGAERVVRAMPNTPALIGEGIAGLYARPAVTPADRAA
                     IEAVLAPTGESLWVAQESDLDAVTALSGSGPAYVFFFIEAMIQAAAEMGLAPEQGRRL
                     AQATFKGATALARASDESPETLRARVTSKGGTTHAAITAMESAGVKEAIVQALRAAQA
                     RAKELGDEFGR"
     gene            complement(529..1428)
                     /gene="ubiA"
                     /locus_tag="VF453_05355"
     CDS             complement(529..1428)
                     /gene="ubiA"
                     /locus_tag="VF453_05355"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX7687107.1"
                     /translation="MPASRVSLYLDLIRWNRPAGWLLLLWPTLAALWIASDGFPGWHL
                     LAVFTLGTILMRSAGCCINDVADRDFDKHVKRTAQRPVTSGALSVRQALGFGAVLALL
                     AFGLVLTTNPPTILLSFVALVVTILYPFAKRVVSMPQAVLGVAFSFGIPMAFSAATGA
                     NDWHPSAIGHAVPGLAWLLLLGNLFWVLAYDTEYAMVDRDDDLKIGIRTSAITLGRLD
                     VAGVMAFYATYLLSWLYLGLKLGLGPAYAVGIVAAALQAGWHFTMIWDRTREGCFRAF
                     RLNHWVGAAVFAGVVLAFLPGVR"
     gene            complement(1469..>1905)
                     /locus_tag="VF453_05360"
     CDS             complement(1469..>1905)
                     /locus_tag="VF453_05360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7687108.1"
                     /translation="RGAMLAELAAAGTPLDLARVHFVGQLPHARYLQALQVSAAHVYL
                     TVPFVLSWSMLEAMACGALVVGSRTSPVEEVIEDGIDGVLVDFFDRDALVAAVLAALR
                     DPARHAPLRAAARAKILARYDLRTRCLPAGLALLEDVAARRT"
BASE COUNT          305 a          700 c          694 g          206 t
ORIGIN      
        1 agcgacgcca tcgcgcgcgc caccggggcc gagcgcgtcg tgcgcgcgat gcccaacacg
       61 ccggcgctga tcggcgaggg catcgccggg ctgtacgcgc ggcccgccgt gacgccggcc
      121 gaccgcgcag ccatcgaggc cgtgctcgcg ccgacgggcg agtcgctgtg ggtggcgcag
      181 gagtccgacc tcgacgccgt cacggcgctc tccggctcgg gtccggccta cgtgttcttc
      241 ttcatcgagg ccatgatcca ggccgccgcc gagatgggcc tggcccccga gcaaggccga
      301 cgcctcgcgc aggccacgtt caagggcgcg accgcgctcg cgcgcgcgtc cgacgaatcg
      361 cccgagaccc tgcgcgcccg cgtgacctcc aagggcggca ccacgcacgc ggcgatcacg
      421 gcgatggagt ccgccggcgt caaggaggcg atcgtgcagg cgctgcgcgc ggcgcaggcc
      481 cgcgccaagg agctcggcga cgaattcggc cgctgatcgc cgcgcgcttc agcgcacgcc
      541 cggcaggaag gccagcacca cgccggcgaa caccgccgcg ccgacccagt ggttcaggcg
      601 aaaggcgcgg aagcagcctt cgcgcgtgcg atcccagatc atcgtgaagt gccagcccgc
      661 ctgcagcgcg gctgccacga tgcccacggc gtacgccggc cccaggccca gcttcaggcc
      721 gaggtacagc cacgacagca ggtaggtcgc gtagaacgcc atcacgcccg cgacatcgag
      781 gcgcccgagc gtgatggccg aggtgcggat gccgatcttc aggtcgtcgt cgcggtcgac
      841 catcgcgtac tcggtgtcgt aggccagcac ccagaacagg ttgcccagca gcagcaacca
      901 cgccaggccc ggcaccgcat ggccgatcgc ggacggatgc cagtcgttcg cgcccgtggc
      961 cgcggagaac gccatgggga tgccgaagct gaacgccacg cccagcaccg cctgcggcat
     1021 cgagacgacg cgcttggcga acggatagag gatggtcacg accagcgcga cgaaggacag
     1081 caggatggtc ggcgggttgg tggtgagcac gagcccgaac gcgagcagcg cgagcacggc
     1141 gccgaagccc agcgcctgtc gcacggacag cgccccgctc gtcaccggcc gctgcgccgt
     1201 gcgcttgacg tgcttgtcga agtcgcggtc ggcgacgtcg ttgatgcagc agccggcgct
     1261 gcgcatcagg atcgtgccga gcgtgaacac cgcgagcagg tgccagcccg ggaagccgtc
     1321 gctggcgatc cacagcgccg ccagcgtcgg ccacaacagc agcagccagc cggcggggcg
     1381 gttccagcgg atcaggtcga ggtagagcga gacgcggctt gcgggcatgg ggcgcattgt
     1441 cgcgcgacgc cccggcccat gccccggctc acgtccggcg cgccgcgacg tcctccagca
     1501 atgccagccc cgccggcagg cagcgcgtgc gcaggtcgta gcgggcgagg atcttcgcgc
     1561 gcgccgccgc ccgcaggggc gcgtggcgcg cggggtcgcg cagcgcggcg aggaccgcgg
     1621 ccacgagcgc gtcgcggtcg aagaagtcga ccagcacgcc gtcgatgccg tcctcgatca
     1681 cctcctcgac cggcgacgtg cggctgccga ccacgagcgc gccgcaggcc atggcctcca
     1741 gcatcgacca cgacagcacg aagggcaccg tcagatacac gtgcgccgcc gagacctgca
     1801 gcgcctgcag gtagcgcgca tggggcagct ggccgacgaa gtgcacgcgc gcgaggtcga
     1861 gcggcgtgcc ggcggcggcg agctcggcga gcatcgcgcc gcgcc
//
LOCUS       DASVLN010000236         3509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_226069, whole genome shotgun sequence.
ACCESSION   DASVLN010000236 DASVLN010000000
VERSION     DASVLN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226069"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..1976
                     /locus_tag="VF453_05365"
     CDS             345..1976
                     /locus_tag="VF453_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7687109.1"
                     /translation="MIDLPAPRPTGRLALSLLAAIALLAGCAALPPAPQPGVDTGPVA
                     LIPRPDPLPSGWEHGAFMEVFVRGYQDSDGDGIGDLRGLTQRLDYLQRLGVRGLWLMP
                     VTASADHDHGYGVVDYRAVERDYGTLADFDELVRQAHARGIGIVMDYVINHSARENPL
                     FQASAASPASPWRDWYVWEPVAPAGWRIFDRDPWTTTPNGAYFAQFGAAMPDFNLRNP
                     KVVAYHLDSLRFWLDHGVDGFRIDAVGHLFENGPQGWSLQPENYAFLRRVRDALDVGR
                     RHVMVCESPGDPAGFARESACGSAFAFGHSGDLVQAGRGDEAAIRKVADYFLSAPDGM
                     ATMVSNHDAFAGPRLWDALDGDAARVRVAAAAYLLQPGTPYLYYGEELGMSHAQEANR
                     DAQLRGPMSWTPDGGFTTGAPFRHPSANLATNNAQAESADPGSMLAFYTAMLGLRNAH
                     PSIALGRYLAPHVDGRVMSFQRAHGAERTLVVINYGDAAGAARVPAWPSNRRLQRLYP
                     APATAAEARLAPARDGDAALVPAPALSVQVFLVAG"
     gene            2256..>3509
                     /locus_tag="VF453_05370"
     CDS             2256..>3509
                     /locus_tag="VF453_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7687110.1"
                     /translation="MPAIPARRRRRWLGTLIALLSIAGLGLLAWHLTHKPKSAYAGGP
                     GGGRRSGGAPATTVGVAAATRADIPVYIDALGTVTPSATVTVTPQVSGVLTKVSFEEG
                     QMVKAGQVLATIDPRPFELALMQAQGAQQKDEAQLEAAQVTLKRYQVLLQQDSIARQD
                     VDTQAALVKQLQGTVLGDKAAVGTARLNLEWTRITAPVAGRIGLRSVDVGNLVNSGSA
                     AGVGTITELQPIDIEFAIPQDRIPALQGRIAAGARLAVTAYDRTRTQVLDTGVFLSLN
                     NQSDTQTGTVRAKARFANARLALFPSQFVNVRVLLDTVQGATVVPVTALRHGPAGDFV
                     YVLDGDHTVSQRTVVQGLSTTDTVQVTEGLAVGEKVITEGGDRLKDGARVVLPGDAPA
                     SGARRRGDGASGASEGWSGRHRRSAE"
BASE COUNT          508 a         1290 c         1240 g          471 t
ORIGIN      
        1 gcagcagggt cgcggaaggc gcgacggcgt cgcaggccgc gagcagcgcg ccctggtcct
       61 gcgtcgcggc gagcgccggc atggcggcaa gcgccgccag cgtcgcgacg caggcccgtt
      121 gcgcgaaacc gagcagcaga gatttcatcc ggaatctggc gacaagtttg agtagtttaa
      181 cttccttcca taagaatgca ctccgacttc aggaaggatt aatacattgt gcttgagagg
      241 catgaccaat gtctagcctg gatacctagt agatttccag atttccgatt cgcatacact
      301 gtaatttcac tacactctcg cgaccgagtg accttggaga cgccatgatc gacctccccg
      361 cgccgcgccc gaccggtcgg ctcgcgctgt ccctgctggc cgccatcgcg ctgctggcag
      421 gctgtgcggc gctcccgcca gcgccgcagc cgggcgtcga caccggcccg gtggccctga
      481 tcccgcggcc cgacccgctg ccttcggggt gggagcacgg tgcattcatg gaggtcttcg
      541 tccgcggcta ccaggacagc gacggcgacg gcatcggcga cctgcgcggg ctcacgcagc
      601 ggctggacta cctccagcgg ctgggcgtgc gcgggctgtg gctcatgccg gtcaccgcca
      661 gcgcggacca tgaccacggc tacggcgtgg tcgactaccg cgcggtggag cgcgactacg
      721 gcacgctcgc cgacttcgac gagctcgtgc gccaggccca cgcgcgcggc atcggcatcg
      781 tgatggacta cgtcatcaac cacagcgcgc gcgagaaccc cctgttccag gcctcggccg
      841 cctcgccggc gagcccctgg cgcgactggt acgtctggga gccggtcgcg cccgcgggct
      901 ggcgcatctt cgaccgggat ccgtggacga cgacgcccaa cggcgcctac ttcgcgcagt
      961 tcggcgcggc gatgccggac ttcaacctgc gcaacccgaa ggtcgtggcc taccacctgg
     1021 acagcctgcg cttctggctg gatcacggcg tcgacggctt tcgcatcgac gccgtcggcc
     1081 acctgttcga gaacggcccc cagggctgga gcctgcagcc ggagaactac gccttcctgc
     1141 gtcgcgtgcg cgacgcgctg gacgtcggcc gccgccacgt catggtgtgc gagtctcccg
     1201 gcgatccggc cggcttcgcg agggaatcgg cctgcggctc cgcgttcgcg ttcggccaca
     1261 gcggcgacct ggtgcaggcc gggcgcggcg acgaggcggc catccgcaag gtcgccgact
     1321 acttcctgag cgcgccggac ggcatggcca cgatggtctc caaccacgac gccttcgccg
     1381 gtccgcggct gtgggacgcg ctggacggcg acgccgcccg cgtgcgcgtg gccgccgccg
     1441 cctacctgct gcagcccggc acgccctacc tgtactacgg ggaggagctc ggcatgtcgc
     1501 acgcgcagga ggccaaccgc gacgcgcagc tgcgcgggcc gatgagctgg acgcccgacg
     1561 gcggcttcac cacgggcgcg ccctttcgcc acccttcggc caacctggcg acgaacaatg
     1621 cgcaggccga gtccgccgac ccgggctcga tgctcgcctt ctacacggcg atgctcgggc
     1681 tgcgcaacgc gcatccgtcg atcgccctcg ggcgctacct cgccccgcac gtggacgggc
     1741 gcgtgatgag cttccagcgc gcccacggcg ccgagcgcac gctggtcgtc atcaactacg
     1801 gcgacgcggc gggtgcggcg cgcgtgccgg catggccgtc gaaccggcgg ctgcagcgcc
     1861 tgtacccggc gcccgcgacg gccgcggagg cgcgcctcgc gcccgcacgc gacggcgacg
     1921 cggccctcgt gccggcgccg gcactgtcgg tgcaggtctt cctggtcgcc ggctgatgcc
     1981 gccggcgggc gtgccccgcg aacgaccgcg accgcggctg cggggtttgc agctcgacag
     2041 gtaaagaaac ctgaagctac cgtacggaca acggccacag gcgtcccgtt tgctatggtt
     2101 gcgcccggct gcaggggtgc ccggcgggcg aggctcgcgt ccgcggcgcc cggcggccgg
     2161 attccgggcg tcggaggatc ccgtccccgc gtgaacgccg cacaaggaca gagatggaaa
     2221 aagaacaagc gcccgccggc tccgcgggcc aggcgatgcc ggcgataccg gcccgccgcc
     2281 gacgccgctg gctcggcacc ctgatcgcgc tgctgtcgat cgccggcctg ggcctgctcg
     2341 cctggcacct cacgcacaag ccgaagagcg cctacgccgg cggcccgggc ggcgggcgcc
     2401 gaagcggcgg cgctccggcg acgacggtcg gggtcgccgc cgcgacccgg gccgacatcc
     2461 cggtctacat cgacgcgctc ggcacggtca cgccctcggc gacggtcacc gtgacgccgc
     2521 aggtctcggg cgtgctgacc aaggtgtcgt tcgaggaagg ccagatggtc aaggcggggc
     2581 aggtgctcgc cacgatcgat ccgcgcccgt tcgagctggc gctgatgcag gcgcagggcg
     2641 cgcagcagaa ggacgaggcg cagctcgagg ccgcgcaggt cacgctcaag cgctaccagg
     2701 tgctgctgca gcaggactcg atcgcgcgcc aggacgtcga cacccaggcc gcgctcgtca
     2761 agcagctgca gggcacggtg ctcggcgaca aggccgccgt cggcacggcc aggctcaacc
     2821 tcgagtggac acgcatcacc gcgcccgtgg ccggccgcat cggcctgcgc agcgtggacg
     2881 tcggcaacct ggtgaactcg ggctcggccg ccggcgtggg cacgatcacc gagctgcagc
     2941 cgatcgacat cgagttcgcg atcccgcagg accgcatccc ggcgctgcag ggccgcatcg
     3001 ccgccggcgc gcggctcgcg gtcaccgcgt acgaccgcac ccgcacgcag gtgctggaca
     3061 cgggcgtgtt cctgtcgctg aacaaccagt ccgacaccca gaccggcacc gtgcgcgcga
     3121 aggcgcgttt cgccaacgcc cggctcgccc tcttcccgag ccagttcgtc aacgtgcgcg
     3181 tgctgctcga caccgtgcag ggtgcgacgg tcgtgccggt caccgcgctg cgccacgggc
     3241 cggcgggcga cttcgtctac gtcctcgacg gcgatcacac ggtcagccag cgcaccgtgg
     3301 tgcaggggct gtcgaccacc gacacggtgc aggtcaccga gggcctggcc gtcggcgaga
     3361 aggtcatcac ggaaggcggc gatcgcctga aggacggcgc ccgggtcgtg ctgccgggcg
     3421 acgccccggc ctcgggcgcc cggcggcgcg gcgacggcgc ttccggcgcg agcgagggct
     3481 ggagcggtcg ccatcgccga tcggccgag
//
LOCUS       DASVLN010000237        14224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_119252, whole genome shotgun sequence.
ACCESSION   DASVLN010000237 DASVLN010000000
VERSION     DASVLN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14224
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119252"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..391
                     /locus_tag="VF453_05375"
     CDS             47..391
                     /locus_tag="VF453_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX7687111.1"
                     /translation="MPADLTLHRDHDLGLARARKVALKWAERIEAELEMECTLIEGDD
                     EDVLEFTRAGVDGRMVVAADRFDIEAKLGFLLRPFLRQIEAEAIRQLDDALAKETAKA
                     AKKTAARAARKQ"
     gene            complement(425..679)
                     /locus_tag="VF453_05380"
     CDS             complement(425..679)
                     /locus_tag="VF453_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HEX7687112.1"
                     /translation="MSTLDDQFQKAVADSKNLPERPDNMTLLKIYALYKQGSEGDVDG
                     KRPGFTDMVGRAKWDAWNELKGKSQDEAKQEYVDLIEGLK"
     gene            857..2269
                     /locus_tag="VF453_05385"
     CDS             857..2269
                     /locus_tag="VF453_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX7687113.1"
                     /translation="MSRVDTAWLRMDTEANLMMIVGVWLLEPQLALAELRQRVETSLL
                     QYRRFRQRVVEDALGAQWIDDEHFDIAAHVVPEKLELAPGQAPLDALKAYVGDLCTTP
                     LNAGRPLWQFHLFEDFETGRTALVARIHHCIADGIALISVMLSITDGGSTPPELARRD
                     EPREEGEHDWVADYLLRPAADLAVKAIGVYGAGLGKGVEMMADPTAPISGGVEMARIG
                     GQIVSDLAAMALMPDDSPTRLKGKATPGKRVAWGEPLPLDEVKAITRGLGASINDVLL
                     ASVAGAIGAYLRGKGDDPTGLEIRAMVPVNLRPLEKAWQLGNRFGLVPLVLPIGIANP
                     IERLAEVRRRMDELKGGYQPLLAFALLSVAGVLIKPVQAALLNLFAKKATAVMTNVPG
                     PAKPLALCGRTLRQVMFWVPASGDIGMGVSILSYGGGVQFGLITDSAMCPDPEEIVRH
                     FRPEYEQMLYLAMMLPWGDA"
     gene            complement(2313..3089)
                     /locus_tag="VF453_05390"
     CDS             complement(2313..3089)
                     /locus_tag="VF453_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687114.1"
                     /translation="MAKWTAFPYDAADYTYDKAALKKHWARLHKGDAEPFPKDEAVQA
                     AWALFHAGEFQKAHEAGLKAGAAGAAVANKAQAIYANYLEKSEKTKLAMFLEVAERAE
                     KLAKDDPKNFAAHYWMAYALGRYSQGISVAKALAQGLGAKVKTALETTIALAPQHADA
                     HIALGAFHAEVIDKVGKLLGKTQGADTATGLKMFKQALALNPDSAIAKVEYANGLVML
                     EGDKRMKEAETLYAEAAAAAPKDAMERLDVEMAKAELEDE"
     gene            complement(3118..3771)
                     /locus_tag="VF453_05395"
     CDS             complement(3118..3771)
                     /locus_tag="VF453_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7687115.1"
                     /translation="MEAKKPRRTAERILEVTLDLFNRFGEPNVSTTLISAELHISPGN
                     LYYHYPAKDELINALFDRYEKSLNELLQAADNVANVEDAWLFFHMLFELIWQYRFLYR
                     DLNDLLSKNRRLETHFQFVLKHKSRAVEAVLDGLSRSSAVRVDRRDVPAIATSMVVVL
                     TYWLSYEYVRDPRKALEPEAAGATLARGAWHVLSLLTPYLEPAAREHLLALAGAYRS"
     gene            3882..4784
                     /locus_tag="VF453_05400"
     CDS             3882..4784
                     /locus_tag="VF453_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7687116.1"
                     /translation="MSAISFQGVEKTYASGVRALDGVTFDVQPGEFFGLLGPNGAGKT
                     SLISILAGLARATRGHVTVMGHHVVDDYAAARRSLGIVPQELVFDPFFSVRETLRIQS
                     GYFGVRNNDAWIDELLAGLGLADKADANMRQLSGGMKRRVLVAQALVHRPPVIVLDEP
                     TAGVDVELRQTLWQFIARLNREGHTVLLTTHYLEEAEALCQRIAMLKKGRVVALERTS
                     ALLAGTAHTMLRFKTDHALPASLAPRARVTGRIAQVSARDAADVEAVLAELRRERVPV
                     EDLEIGRADLEDVFLSIMTAEAAQ"
     gene            4781..5596
                     /locus_tag="VF453_05405"
     CDS             4781..5596
                     /locus_tag="VF453_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7687117.1"
                     /translation="MSSLPSSRPRVSSLSTARGILAGAQPLFRKEVLRFWKVAFQTVA
                     APVLTAVMYLVVFGHALTGRLSAYPGVSYVQFLIPGLVMMSVLQNAFANSSSSLIQSK
                     VTGNLVFLLVTPLSPAAWFLAYTGAAVVRGLVVGAGVMLVTAWFAPPGLASAPWILVF
                     AVLGAAMMGTLGLIAGLWAEKFDQLAAFQNFIIMPLTFLSGVFYSVHSLPPLWHFASR
                     LNPFFYMIDGFRHGFFGASDVSPWTSLAVVGGSLAVVAAIALALLRSGYKLRH"
     gene            5648..5929
                     /locus_tag="VF453_05410"
     CDS             5648..5929
                     /locus_tag="VF453_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX7687118.1"
                     /translation="MSDPTPQEVHGFIAGGLQCDHLEVEGDGRHFFATIVSAEFEGLA
                     RVRRHQRVYAALGDRMRAQIHALSMKTLTPAEWSAQGALGNAAGHHHSH"
     gene            6066..7358
                     /gene="murA"
                     /locus_tag="VF453_05415"
     CDS             6066..7358
                     /gene="murA"
                     /locus_tag="VF453_05415"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7687119.1"
                     /translation="MDKLLIRGGRRLNGEVAISGAKNAALPELCATLLAAGAVTLHNV
                     PRLQDVATVVKLIRNMGVAVRWGAEQDGGAADEVVVDASVLTSPEAPYELVKTMRASI
                     LVLGPLLARFGEATVSLPGGCQIGSRPVDQHIKGLQAMGADIRVEHGYILARAARLKG
                     ARITTDMVTVTGTENLLMAATLAEGETILENAAQEPEIPDLAELLIAMGARIEGHGTS
                     RIRIQGVEGLHPPARPHRIVPDRIEAGTFLCAVAAAGGDVTLRDAEAAHLEAVIEKLR
                     EAGVSIEAGDRWIRVRSPMGEAGGRLRAVSLRTSEYPAFPTDMQAQFMAVDCIAQGTA
                     TVTETIFENRFMHVDELRRLGARIEIDGKHAVVAGVERLSGATVMATDLRASASLVIA
                     GLVADGQTIVDRIYHLDRGYDRMEAKLQRLGADIERVK"
     gene            7379..8038
                     /gene="hisG"
                     /locus_tag="VF453_05420"
     CDS             7379..8038
                     /gene="hisG"
                     /locus_tag="VF453_05420"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX7687120.1"
                     /translation="MLTLALSKGRIFEETLPLLAAAGIEVTEDPEKTRKLILATNQPD
                     VRVVLVRATDVPTYVQHGGADVGVAGKDVLIEHAAGADGNGGLYQPLDLRIARCRMSV
                     ATRADFDYEAAVKQGSRIRVATKYTAVASRHFADKGVHVDLIKLYGSMELAPLTGLAD
                     AIVDLVSTGSTLKANRLVEVERIMDISSRLVVNQAALKLKRERIRRLIDAFAGAIDKN
                     A"
     gene            8067..9383
                     /gene="hisD"
                     /locus_tag="VF453_05425"
     CDS             8067..9383
                     /gene="hisD"
                     /locus_tag="VF453_05425"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX7687121.1"
                     /translation="MPVQIRQLATSIADFEARFAELRHWSEEADHAIEERVAAILADV
                     RARGDAAVLEYTARFDGLQADAVAGLELARDELEAAFEAITPAQRAALESAARRVRSY
                     HERQLEACGRSWQYRDEDGTLLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAVPAQVAG
                     VGEIVMVVPTPRGERNPLVLAAAYVAGVHRAFTVGGAQAVAALAYGTATIPAVDKITG
                     PGNAYVASAKRRVFGQVGIDMIAGPSEILVLADGTTPADWVAMDLFSQAEHDELAQSI
                     LLCPDAGYVEQVKAAIDRLLPDMPRRDVIRASLEGRGALIHTHSMDEACAISNRIAPE
                     HLEVSSADPHRWEPQLRHAGAIFLGAYTSESLGDYCAGPNHVLPTSGTARFSSPLGVY
                     DFQKRSSLIEVSEAGAQVLGPVAAELAYGEGLQGHARAAELRLKNT"
     gene            9468..9686
                     /locus_tag="VF453_05430"
     CDS             9468..9686
                     /locus_tag="VF453_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX7687122.1"
                     /translation="MKNVTVTMEDQVADWARMEAARRNTSVSRLIGELLAEKMRHDDS
                     YERAMREALEFQSFGRSDGSRFERGANR"
     gene            9692..10162
                     /locus_tag="VF453_05435"
     CDS             9692..10162
                     /locus_tag="VF453_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7687123.1"
                     /translation="MPASAPPRVFIDTGVLLHCVDDHDTRRRDRARAWLSSCWTARCG
                     RTSMQVLSEFYAHARRHFPTAISAGDARAFVRRYQHWKPWIVDQATIDTAWAIESRHE
                     LPYFDALILAAAQQQECRFIASDRWVHGRQIETVTVVDPFASSFDALDTMGTPA"
     gene            10183..11268
                     /gene="hisC"
                     /locus_tag="VF453_05440"
     CDS             10183..11268
                     /gene="hisC"
                     /locus_tag="VF453_05440"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7687124.1"
                     /translation="MSRYLRADVQGMHGYAVQPSAGFVKVDTMENPFPLPPDLRRALG
                     ERLAEVALNRYPAERGDVLRAELAKCARMPGDCDIMLGNGSDELISLLTLAADVPGNV
                     VLAPVPGFVMYEMAARLQGLKFVGVPTTADFELDGPAMLAAIREHQPAIVWLAYPNNP
                     TANLWDDAVIDAIIEAAPGLVVIDEAYQPFAARGSLERLRRHEHVLLMCTMSKFGLAG
                     VRIGYLVGRRPLIAEIDKLRPPFNVSVLNCEAALFALEHADEYARQAATIRAERAKLF
                     DALAATPGVKPFPSEANMILARVPDAKRVFEGMKARGVLVKNVSGLHPLLANCVRITI
                     GTPDENPLTLAALRGALQELGDLEKAE"
     gene            11265..11873
                     /gene="hisB"
                     /locus_tag="VF453_05445"
     CDS             11265..11873
                     /gene="hisB"
                     /locus_tag="VF453_05445"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX7687125.1"
                     /translation="MTIAPSPARVAAVQRDTKETKIKVRVNLDGTGTARLQTGIGFFD
                     HMLDQIARHGLIDLEIHADGDLHIDGHHTVEDVGITLGQAVAQAVGDKKGLTRYGHAY
                     VPLDEALSRVVVDFSGRPGLHMDVKFTSGSVGAFDTQLAFEFFQGFSNHAGVSLHIDN
                     LKGHNAHHQCETIFKAFGRALRMAMTLDERSAGVIPSTKGSL"
     gene            11870..12538
                     /gene="hisH"
                     /locus_tag="VF453_05450"
     CDS             11870..12538
                     /gene="hisH"
                     /locus_tag="VF453_05450"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX7687126.1"
                     /translation="MTTGGRGAPSTVAVVDYGMGNLRSVAQAVMKAAEGQGVDVVIAR
                     TADEVLAAERVVLPGQGAIRDCLRELRDSGMQDAVLDAAARKPLLGVCVGMQMLLDRS
                     EEQGQPGLGLIPGEVRRFRLEGRLQDDGSRYKVPQMGWNRVRQDVAHPLWAGIPDGSW
                     FYFVHSFYATTVDPAHGAGTTDYGGRFTSAVARDNIFATQFHPEKSAALGLRLYRNFL
                     HWTP"
     gene            12554..13297
                     /gene="hisA"
                     /locus_tag="VF453_05455"
     CDS             12554..13297
                     /gene="hisA"
                     /locus_tag="VF453_05455"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX7687127.1"
                     /translation="MLLIPAIDLKDGHCVRLEQGDMSAATSFGDDPAAMARRWLEAGA
                     RRLHLVDLNGAFAGKPVNESAVKAIIKEVDGRIPIQLGGGIRDLDTIERYLDDGLSYI
                     IIGTAAVKSPGFLKDACTAFGGHIIVGLDAKDGKVATDGWSKLTGHEVVDLALKFQDY
                     GVEGVIYTDIGRDGMLTGINIEATVKLAQALTIPVIASGGLSGLADIEQLCAVESEGV
                     EGVICGRSIYTGALDFAHAQSRADELLAE"
     gene            13359..14138
                     /gene="hisF"
                     /locus_tag="VF453_05460"
     CDS             13359..14138
                     /gene="hisF"
                     /locus_tag="VF453_05460"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX7687128.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGA
                     DELTFLDITATSDGRDLILPIIEAVASQVFIPLTVGGGVREVADVRRLLNAGADKVSF
                     NSAAVANPQVIAEASDRYGAQCIVVAIDAKRRSDEDAATRGPGWDVYTHGGRKNTGLD
                     AVEWAREMARRGAGEILLTSMDRDGTRAGFDLALTRAVADAVPVPVIASGGVGSLEHL
                     ADGVQQGGADAVLAASIFHYGQHTVGEAKALMAARGIPVRR"
BASE COUNT         2076 a         5114 c         4974 g         2060 t
ORIGIN      
        1 ctgacgcgtt ctggtccaga atgtcgtccc gaggagacac gcccacatgc ccgccgacct
       61 gaccctgcac cgcgaccacg acctcggcct cgcccgcgcg cgcaaggtgg cgctgaagtg
      121 ggccgagcgc atcgaggcgg agctcgagat ggaatgcacc ctgatcgagg gcgacgacga
      181 agacgtgctg gagttcacgc gcgccggcgt cgatggccgc atggtcgtgg cggccgatcg
      241 cttcgacatc gaggcgaagc tgggcttcct gctgcgaccc ttcctcaggc agatcgaggc
      301 cgaggcgatc cgccagctgg acgacgcgct tgccaaggag accgccaagg cggcgaagaa
      361 gacggcggcg agggccgcga ggaagcagta gcggcgtgac ggccggcgcg cgccggccgc
      421 ggcctcactt caggccttcg atcaggtcga cgtattcctg cttggcctcg tcctgcgact
      481 tgcccttgag ctcgttccag gcgtcccact tggcgcggcc gaccatgtcg gtgaagcccg
      541 ggcgcttgcc gtcgacgtcg ccctcgctgc cctgcttgta caacgcgtag atcttcagca
      601 gcgtcatgtt gtccggacgc tcgggcaggt tcttgctgtc ggccaccgcc ttctggaact
      661 ggtcgtcgag ggtgctcatt gcgtgtctcc gcgtgggtga aagtcaggct tgacgggccg
      721 gatgatagac accaatctgg ccgccctcgc gaaataatgt cgagagagca gtccagccgc
      781 gcgcgccttc cggaatcggc cggggcgtcg atccgcggcg ggcaggggga gacgcttgag
      841 cagcaacgtt gaacgcatgt cccgggtcga cacggcctgg ctgcgcatgg acaccgaggc
      901 caacctgatg atgatcgtcg gggtgtggct gctggagccc cagctcgcgc tcgccgagct
      961 gcgccagcgc gtcgagacca gcctgctgca gtaccggcgc ttccgccagc gggtggtcga
     1021 ggacgcgctc ggcgcccagt ggatcgacga cgagcacttc gacatcgccg cccacgtcgt
     1081 ccctgaaaag ctcgagctcg cgcccggcca ggcgccgctg gacgcgctca aggcctacgt
     1141 cggcgacctg tgcacgacgc cgctgaacgc cggccgcccg ctgtggcagt tccacctgtt
     1201 cgaggatttc gagaccggcc gcacggcgct cgtcgcgcgc atccaccact gcatcgccga
     1261 cggcatcgcg ctgatctcgg tgatgctgtc catcacggac ggcggcagca cgccgcccga
     1321 gctcgcccgg cgcgacgagc cgcgcgagga gggcgagcac gactgggtcg ccgactacct
     1381 gctccggccc gcggccgacc tcgcggtcaa ggcgatcggc gtctacggcg cgggcctggg
     1441 caagggcgtc gagatgatgg ccgacccgac cgccccgatc tcgggcggcg tggagatggc
     1501 gcgcatcggc ggccagatcg tcagcgacct cgcggcgatg gcgctgatgc ccgacgactc
     1561 gcccacgcgc ctgaagggca aggccacgcc gggcaagcgc gtcgcctggg gcgagccgct
     1621 gccgctcgac gaggtcaagg cgatcacccg cggactgggc gcgtcgatca acgacgtgct
     1681 gctcgcgagc gtggccggcg cgatcggcgc ctacctgcgc ggcaagggcg acgacccgac
     1741 cggcctcgag atccgcgcga tggtgccggt caacctgcgg ccgctcgaga aggcctggca
     1801 gctgggcaac cgcttcgggc tcgtgccgct ggtgctgccg atcggcatcg ccaacccgat
     1861 cgagcggctc gccgaggtgc gccgccgcat ggacgaactc aagggcggct accagccgct
     1921 gctcgccttc gcgctgctgt cggtggcggg cgtgctgatc aagccggtgc aggccgcgct
     1981 gctgaacctg ttcgcgaaga aggccaccgc ggtgatgacc aacgtcccgg gcccggccaa
     2041 gccgctcgcg ctgtgcggcc gcaccttgcg gcaggtgatg ttctgggtgc ccgcctcggg
     2101 cgacatcggc atgggcgtga gcatcctcag ctacggcggc ggcgtgcagt tcggtctcat
     2161 caccgactcc gcgatgtgcc ccgaccccga ggagatcgtc cgccacttcc ggccggagta
     2221 cgagcagatg ctgtacctgg cgatgatgct gccctggggc gacgcctgaa ccctggccga
     2281 ggcccgcccc gccgggtgcg cggggcggcg cgtcactcgt cctcgagctc cgccttcgcc
     2341 atctccacgt ccagccgctc catcgcgtcc ttcggcgcgg cggcggcggc ctcggcatag
     2401 agggtctcgg cttccttcat gcgcttgtcg ccctcgagca tcacgaggcc gttggcgtat
     2461 tccaccttcg cgatggccga gtcggggttc agcgcgaggg cctgcttgaa catcttcagg
     2521 ccggtggcgg tgtccgcgcc ctgcgtcttg ccgaggagct tgccgacctt gtcgatcacc
     2581 tcggcgtgga aggcgccgag cgcgatgtgg gcgtcggcgt gctggggggc cagcgcgatc
     2641 gtcgtctcga gcgcggtctt gaccttggcg cccaggccct gcgccagcgc cttggccacg
     2701 ctgatgccct ggctgtagcg gcccagcgcg taggccatcc agtagtgggc ggcgaagttc
     2761 ttcgggtcgt ccttggcgag cttctcggcg cgctcggcga cctccaggaa catcgcgagc
     2821 ttggtcttct cgctcttctc gaggtagttg gcgtagatcg cctgcgcctt gttggcgacc
     2881 gccgcgcccg cagcgcccgc cttcaggccg gcctcgtgcg ccttctggaa ctcgcccgcg
     2941 tggaacaggg cccacgcggc ctggacggcc tcgtccttcg ggaagggctc ggcgtcgccc
     3001 ttgtgcaggc gcgcccagtg cttcttcagc gccgccttgt cgtaggtgta gtcggcggcg
     3061 tcgtagggga aggcggtcca cttggccatg cgtgtctccg gtgcgggtcg ggaggtttca
     3121 ggaacggtag gcgcccgcca gggcgagcag gtgctcgcgc gcggccggct ccaggtaggg
     3181 cgtgagcagg ctcagcacgt gccacgcgcc gcgcgcgagc gtcgcgccgg cggcctcggg
     3241 ctcgagcgcc ttgcgcgggt cgcgcacgta ctcgtagctg agccagtagg tgagcacgac
     3301 caccatcgag gtcgcgatcg cgggcacgtc gcggcggtcg acgcgcaccg cgctgctgcg
     3361 gctcaggccg tcgagcacgg cctcgacggc gcgcgacttg tgcttgagga cgaactggaa
     3421 gtgcgtctcc agtcggcggt tcttcgacag caggtcgttg aggtcgcggt acaggaagcg
     3481 gtactgccag atcagctcga acagcatgtg gaagaacagc caggcgtcct ccacgttcgc
     3541 cacgttgtcg gcggcctgca gcagctcgtt gagcgacttc tcgtagcggt cgaagagcgc
     3601 gttgatcagc tcgtccttcg ccgggtagtg gtagtacagg ttgcccgggc tgatgtgcag
     3661 ctccgccgag atcagcgtcg tcgacacgtt cggctcgccg aagcggttga acaggtcgag
     3721 cgtcacctcg aggatgcgtt cggcggtgcg acggggtttc ttggcttcca tggggccggc
     3781 ggcggtgcgg atcgcgcaat ctagcatcgc ggccgcgcgc tgcccagccg gagtgccact
     3841 gttggagcgc gctctccagg cttgcgccac aatccgcgcc catgtcagcg atttcgttcc
     3901 agggcgtgga gaagacctac gccagcggcg tccgggcgct ggatggcgtc acgttcgacg
     3961 tccagcccgg cgagttcttc ggcctgctcg gccccaacgg cgcgggcaag acctcgctga
     4021 tcagcatcct cgcgggcctc gcgcgcgcca cgcgcggcca cgtcacggtg atgggccacc
     4081 acgtcgtcga cgactacgcc gccgcccgcc gctcgctggg catcgtgccg caggagctcg
     4141 tgttcgaccc gttcttctcg gtgcgcgaga cgctgcgcat ccagagcggc tacttcggcg
     4201 tgaggaacaa cgacgcctgg atcgacgagc tgctcgcggg cctgggcctc gcggacaagg
     4261 ccgatgccaa catgcgccag ctctcgggcg gcatgaagcg gcgcgtgctg gtggcgcagg
     4321 ccctcgtgca ccggccgccg gtcatcgtgc tggacgagcc gaccgcgggc gtggacgtcg
     4381 agctgcgcca gaccctgtgg cagttcatcg cgcgcctgaa ccgcgagggc cacaccgtgc
     4441 tgctgaccac gcactacctc gaggaggccg aggcgctgtg ccagcgcatc gcgatgctca
     4501 agaagggccg ggtcgtcgcg ctggagcgca cctccgcgct cctggcgggc accgcgcaca
     4561 cgatgctgcg cttcaagacc gaccacgcgc tgcccgcgag cctcgcgccg cgcgcgcgcg
     4621 tgaccggccg catcgcccag gtgagcgcgc gcgacgccgc cgacgtcgag gccgtgctcg
     4681 ccgagctgcg gcgcgagcgg gtgccggtcg aggacctcga gatcggccgc gccgacctcg
     4741 aggacgtctt cctctcgatc atgaccgcgg aggcggcgca atgagcagcc tcccgtcgtc
     4801 gcgcccgcgg gtctcgagcc tgtcgaccgc ccgcggcatc ctcgccggcg cccagccgct
     4861 gttccgcaag gaggtgctgc gcttctggaa ggtggcgttc cagaccgtcg ccgcgccggt
     4921 gctgacggcg gtgatgtacc tggtcgtctt cggccacgcg ctcaccggcc gcctgagcgc
     4981 ctaccccggc gtgagctacg tgcagttcct catccccggc ctcgtgatga tgagcgtgct
     5041 tcagaacgcg ttcgccaaca gctcgtcctc gctgatccag agcaaggtca cgggcaacct
     5101 cgtgttcctg ctcgtgacgc cgctgtcgcc cgcggcctgg ttcctcgcct acaccggcgc
     5161 cgcggtggtg cgcgggctcg tcgtcggcgc gggcgtgatg ctcgtgaccg cgtggttcgc
     5221 cccgccgggg ctcgcgagcg cgccgtggat cctcgtcttc gccgtgctcg gcgcggccat
     5281 gatgggcacc ctcggcctga tcgcgggcct gtgggcggag aagttcgacc agctcgccgc
     5341 gttccagaac ttcatcatca tgccgctgac cttcctgtcg ggcgtcttct actcggtgca
     5401 ctcgctgccg ccgctgtggc acttcgcgag ccggctcaac ccgttcttct acatgatcga
     5461 cggcttccgc cacggcttct tcggcgcgag cgacgtctcg ccgtggacga gcctggccgt
     5521 ggtcggcggc agcctcgccg tggtcgcggc gatcgcgctg gcgctgctgc ggtccggcta
     5581 caagctgcgg cattgagaat cccggcgctg gcgggccgcc gccggcatga gggataatcc
     5641 cgatcccatg tccgatccca cgccccagga agtccacggc ttcatcgccg gcggcctgca
     5701 atgcgaccac ctcgaggtcg agggcgacgg tcgccacttc ttcgccacca tcgtctcggc
     5761 ggagttcgag ggcctcgcgc gcgtgcgccg tcaccagcgg gtctacgcgg cgctcggcga
     5821 ccggatgcgg gcgcagatcc acgcgctgtc gatgaagacg ctgacgccgg ccgagtggtc
     5881 ggcgcagggt gcgctcggca acgccgccgg ccatcaccat tcgcactgac gcccgccgcg
     5941 ccgcctgacg cgcgctgcgg gacgccttcg cgccgcggcc ggcgcgggtg tcccgggacg
     6001 acgcggcagc cggcggggcc ttccaccccg acacgctcgc gcagccttcc cagcaagaca
     6061 tcccgatgga caaactcctc atccgcggcg gccggcgcct gaacggcgag gtcgcgatct
     6121 ccggcgcgaa gaacgcggcg ctgcccgagc tgtgcgcgac gctgctggcc gcgggcgcgg
     6181 tgacgctgca caacgtgccg cggctgcagg acgtcgcgac cgtggtcaag ctgatccgca
     6241 acatgggcgt cgccgttcgc tggggggccg agcaggacgg cggcgcggcg gacgaggtcg
     6301 tcgtggacgc gagcgtgctg acctcgcccg aggcgcccta cgagctcgtg aagacgatgc
     6361 gcgccagcat cctggtgctc gggccgctgc tcgcccgctt cggcgaggcg acggtgtcgc
     6421 tgccgggcgg ctgccagatc ggctcgcgcc cggtcgacca gcacatcaag ggcctgcagg
     6481 cgatgggcgc ggacatccgc gtcgagcacg gctacatcct cgccagggcg gcgcggctga
     6541 agggcgcgcg catcacgacc gacatggtca cggtcaccgg caccgagaac ctgctgatgg
     6601 ccgcgacgct ggccgagggc gagaccatcc tcgagaacgc cgcgcaggag cccgagatcc
     6661 ccgacctcgc cgagctgctg atcgcgatgg gcgcgcgcat cgagggccac ggcacgagcc
     6721 gcatccgcat ccagggcgtc gagggcctgc acccgccggc gcggccgcac cgcatcgtgc
     6781 ccgatcgcat cgaggccggc accttcctct gcgcggtggc cgcggccggc ggcgacgtca
     6841 cgctgcgcga cgccgaggcg gcgcacctgg aggcggtgat cgagaagctg cgcgaggccg
     6901 gcgtctcgat cgaggcgggc gaccgctgga tccgcgtgcg ctcgccgatg ggcgaggccg
     6961 gcggccggct gcgcgccgtc agcctgcgca ccagcgagta cccggccttc ccgaccgaca
     7021 tgcaggccca gttcatggcc gtcgactgca tcgcccaggg caccgcgacc gtcaccgaga
     7081 cgatcttcga gaatcgcttc atgcatgtcg acgagctgcg ccgcctgggc gcgcgcatcg
     7141 agatcgacgg caagcacgcg gtggtcgcgg gcgtcgagcg gctgtcgggc gcgaccgtga
     7201 tggccaccga cctgcgcgcc tcggcgagcc tcgtgatcgc cggcctcgtc gccgacggcc
     7261 agaccatcgt cgaccgcatc taccacctgg accgcggcta cgaccgcatg gaagccaagc
     7321 tgcagcgcct cggcgcggac atcgagagag tgaagtgacc cccaccacga cgacgcccat
     7381 gctcacgctc gccctgtcca agggccgcat cttcgaggag accctcccgc tgctggcggc
     7441 cgccggcatc gaggtgaccg aggatccgga gaagacgcgc aagctgatcc tcgcgaccaa
     7501 ccagcccgac gtgcgcgtcg tgctggtgcg cgcgaccgac gtgcccacct atgtccagca
     7561 cggcggcgcc gacgtcggcg tcgcgggcaa ggacgtgctg atcgagcacg ccgccggcgc
     7621 cgacggcaac ggcgggctct accagccgct ggacctgcgc atcgccaggt gccgcatgag
     7681 cgtggccacg cgcgcggact tcgactacga agccgcggtc aagcagggct cgcgcatccg
     7741 cgtcgcgacg aagtacaccg ccgtcgcgag ccgccacttc gccgacaagg gcgtgcacgt
     7801 cgacctcatc aagctctacg gctcgatgga gctcgcgccg ctcaccggcc tggccgacgc
     7861 gatcgtcgac ctcgtctcga ccggctcgac gctgaaggcg aaccgcctgg tggaggtcga
     7921 gcgcatcatg gacatctcct cccgcctggt cgtcaaccag gccgcgctca agctcaagcg
     7981 cgagcgcatc cgccggctga tcgacgcctt cgcgggcgcg atcgacaaga acgcctgacg
     8041 cctccaactc caccgacgcc actgccatgc cggtccagat ccgccagctc gccacgtcca
     8101 tcgccgattt cgaagcccgc ttcgccgagc tgcgccattg gtccgaggag gccgaccatg
     8161 cgatcgagga gcgcgtcgcc gcgatcctcg ccgacgtgcg cgcgcgcggc gacgccgcgg
     8221 tgctcgagta cacggcgcgc ttcgacggcc tgcaggccga cgccgtcgcc ggcctggaac
     8281 tggcccgtga cgagctggag gccgcgttcg aggcgatcac gccggcgcag cgggcggcgc
     8341 tggaatcggc ggcgcggcgg gtgcggtcgt accacgaacg ccagctcgag gcctgcggcc
     8401 gcagctggca ataccgcgac gaggacggca cgctgctcgg ccagaaggtg acgccgctcg
     8461 atcgcgtcgg catctacgtg ccgggcggca aggccgccta cccgtccagc gtgctgatga
     8521 acgcggtgcc ggcgcaggtc gccggcgtcg gcgagatcgt gatggtcgtc ccgacgccgc
     8581 gcggcgagcg caaccccctc gtgctggccg cggcctacgt cgccggcgtg catcgcgcgt
     8641 tcacggtcgg cggcgcgcag gccgtggccg cgctcgcgta cggcaccgcg accatcccgg
     8701 ccgtcgacaa gatcaccggc cccggcaacg cgtacgtcgc gagcgcgaag cgccgcgtgt
     8761 tcggccaggt gggcatcgac atgatcgccg gtccgagcga gatcctcgtg ctcgcggacg
     8821 gcacgacgcc ggccgactgg gtcgcgatgg acctgttcag ccaggccgag cacgacgagc
     8881 tcgcgcaaag catcctgctg tgtcccgacg ccggctacgt cgagcaggtc aaggcggcga
     8941 tcgaccgcct gctgcccgac atgccgcgcc gcgacgtgat ccgcgcgtcg ctcgagggcc
     9001 gcggcgccct gatccacacg cattccatgg acgaggcgtg cgcgatcagc aaccgcatcg
     9061 cgccggagca cctggaggtc agcagcgccg acccgcaccg gtgggagccg cagctgaggc
     9121 atgccggcgc catcttcctc ggcgcgtaca cgagcgagag cctgggcgac tactgcgccg
     9181 gccccaacca cgtcctgccc acctcgggca cggcgcgctt ctcgtcgccg ctgggcgtct
     9241 atgacttcca gaagcgcagc agcctgatcg aggtgagcga ggccggggcc caggtactcg
     9301 ggccggtcgc ggccgagctc gcgtacggcg aggggctgca gggacatgcc cgggccgcag
     9361 agctgcggct gaagaacacc tgatctttcg cgacactgag ttcgtgcaac acgaattcag
     9421 tgtgacacga agttcgtgta ttcttcgatc caaagactgg atccgcgatg aagaacgtca
     9481 ccgtcaccat ggaagaccag gtcgccgact gggcacggat ggaagccgct cgccgcaaca
     9541 caagcgtctc gcgcctcatc ggcgagctgt tggcggagaa gatgcgccac gacgattcct
     9601 acgaacgcgc gatgcgcgag gcgctggagt tccagagctt cgggcgctcg gacggcagcc
     9661 gcttcgagcg cggggcgaac cggtgagcgc ggtgccggcc tccgcgccgc cccgggtctt
     9721 catcgacacg ggggtcctgc tgcactgcgt ggacgaccat gacacgcggc gccgtgatcg
     9781 cgcgcgagcc tggctctcga gctgctggac ggcgcgttgc ggccgcacga gcatgcaggt
     9841 cctctccgag ttctatgccc acgcccgccg gcacttcccg accgcgatct cggcgggaga
     9901 cgcccgcgcg ttcgtgcgcc gctaccagca ctggaagccc tggatcgtcg accaggccac
     9961 catcgacacg gcctgggcga tcgaaagccg ccacgagctc ccctacttcg acgcgctgat
    10021 cctggcagcc gcgcagcagc aggagtgccg cttcatcgcc tccgaccgct gggtgcacgg
    10081 gcggcagatc gagacggtga ccgtggtcga cccgttcgca tcctccttcg acgccctgga
    10141 cacgatggga acccccgcat gacgacagcc ctctccgccc gcatgtcccg ctacctccgc
    10201 gccgacgtcc agggcatgca cggctacgcc gtccagccca gcgccggctt cgtcaaggtc
    10261 gacacgatgg agaacccgtt cccgctgccg cccgacctgc ggcgcgcgct gggcgagcgg
    10321 ctcgccgagg tcgcgctcaa ccgctacccg gccgaacgcg gcgacgtgct gcgcgccgag
    10381 ctcgcgaaat gcgcccgcat gccgggcgac tgcgacatca tgctgggcaa cggctccgac
    10441 gagctgatca gcctgctgac gctggccgcc gacgtgccgg gcaacgtggt gctcgcgccg
    10501 gtgccgggct tcgtgatgta cgagatggcg gccaggctgc agggcctgaa gttcgtcggc
    10561 gtgccgacga cggccgactt cgagctcgac ggccccgcga tgctcgccgc gatccgcgag
    10621 caccagccgg ccatcgtctg gctcgcctac ccgaacaacc cgaccgcgaa cctgtgggac
    10681 gacgccgtca tcgacgcgat catcgaggcc gcgcccggcc tggtcgtgat cgacgaggcc
    10741 taccagccgt tcgcggcgcg cggctcgctc gagcgcctgc gccgccacga gcacgtgctg
    10801 ctgatgtgca cgatgagcaa gttcggcctc gcgggcgtgc gcatcggcta cctcgtcggt
    10861 cgcaggccgc tgatcgccga gatcgacaag ctgcgcccgc cgttcaacgt cagcgtgctc
    10921 aattgcgagg cggcgctgtt cgcgctcgag catgccgacg aatacgcgcg ccaggccgcg
    10981 acgatccgcg ccgagcgcgc gaagctgttc gacgcgctcg cggcgacgcc cggcgtgaag
    11041 ccgttcccga gcgaggccaa catgatcctc gcgcgcgtgc ccgacgcgaa gcgcgtcttc
    11101 gagggcatga aggcgcgcgg ggtgctggtc aagaacgtgt cgggcctgca cccgctgctc
    11161 gccaactgcg tgcgcatcac catcggcacg cccgacgaga acccgttgac cctcgccgcg
    11221 ctgcgcggcg ccctgcaaga gctcggcgac ctggagaaag ctgaatgacc atcgccccgt
    11281 caccggcccg cgtggccgcc gtccagcgcg acaccaagga aacgaagatc aaggtgcgcg
    11341 tcaacctcga cggcaccggc accgcccgcc tgcagacggg catcggcttc ttcgaccaca
    11401 tgctcgacca gatcgcgcgc cacgggctga tcgacctcga gatccatgcc gacggcgacc
    11461 tgcacatcga cggccaccac accgtcgagg atgtcggcat caccctcggc caggccgtcg
    11521 cgcaggcggt cggcgacaag aagggcctga cgcgctacgg ccacgcctac gtgccgctgg
    11581 acgaggcgct ctcgcgcgtg gtggtcgact tctcgggccg ccccggcctg cacatggacg
    11641 tgaagttcac ttccggctcg gtcggcgcgt tcgacaccca gctcgcgttc gagttcttcc
    11701 agggcttctc gaaccacgcg ggggtgtcgc tgcacatcga caacctgaag ggccacaacg
    11761 cgcatcacca gtgcgagacg atcttcaagg cgttcgggcg cgcgctgcgc atggcgatga
    11821 cgctcgacga gcgttccgcg ggcgtgatcc cgtcgacgaa gggctcgctg tgaccacggg
    11881 cggacgaggg gccccttcga cggtcgccgt cgtcgactac ggcatgggca acctgcgctc
    11941 ggtggcgcag gcggtcatga aggcggcgga ggggcagggg gtcgacgtcg tcatcgcgcg
    12001 cacggccgac gaggtgctgg cggccgagcg cgtcgtgctg ccgggccagg gcgcgatccg
    12061 cgactgcctg cgcgagctgc gcgactcggg catgcaggac gcggtgctcg acgcggccgc
    12121 gcgcaagccg ctgctgggcg tgtgcgtggg catgcagatg ctgctcgatc gcagcgagga
    12181 gcagggccag cccggcctcg ggctgatccc gggcgaggtg cggcgcttcc gcctcgaggg
    12241 ccgcctgcag gacgacggca gccgctacaa ggtgccgcag atgggctgga accgcgtgcg
    12301 gcaggacgtc gcgcatccgc tgtgggcggg cattccggac ggctcctggt tctacttcgt
    12361 ccacagcttc tatgcgacga ccgttgatcc ggctcacggc gcgggcacga ccgactacgg
    12421 cgggcgcttt acctctgccg tggcccggga taacattttc gccacccaat tccaccccga
    12481 gaagagcgcg gccctgggcc tgcggctcta tcgcaacttc ctgcactgga cgccctgacg
    12541 gggcccgcgc gtcatgctgt tgattcccgc gatcgatctg aaggacggcc actgcgtccg
    12601 cctcgaacag ggcgacatgt ccgccgccac cagcttcggc gacgatcctg ccgcgatggc
    12661 ccggcgatgg ctggaggccg gggcccgccg gctgcacctg gtcgacctga acggcgcgtt
    12721 cgccggcaag ccggtcaacg aatcggcggt caaggcgatc atcaaggaag tcgacggccg
    12781 catccccatc cagctgggcg gcggcatccg cgacctcgac accatcgagc gctacctcga
    12841 cgacggcctg agctacatca tcatcggcac ggccgcggtc aagagcccgg gcttcctgaa
    12901 ggacgcctgc accgcgttcg gcggccacat catcgtcggc ctggacgcca aggacggcaa
    12961 ggtcgcgacc gacggctgga gcaagctcac cggccacgag gtcgtcgacc tggcgctcaa
    13021 gttccaggac tacggcgtcg agggcgtgat ctacaccgac atcggccgcg acgggatgct
    13081 cacgggcatc aacatcgagg ccaccgtgaa gctcgcgcag gcgctcacga tcccggtgat
    13141 cgcctcgggc ggcctgtcgg ggctggccga catcgagcag ctgtgcgcgg tcgagtccga
    13201 gggcgtcgaa ggcgtgatct gcggccgctc gatctacacg ggcgcgctcg acttcgcgca
    13261 cgcgcagtcg cgcgccgacg aactcctcgc cgagtgaccc tgcgcccgcg ggcgcttctc
    13321 caccccgaaa ggcggcgggc tcgtcccgcc ggagcgccat gctcgccaaa cgcatcatcc
    13381 cctgcctgga cgtgaccggc ggccgcgtcg tcaaaggcgt gaacttcgtc gagctgcgcg
    13441 atgccggcga cccggtcgag atcgcggccc gatacaacga gcagggcgcc gacgaactga
    13501 ccttcctcga catcaccgcg accagcgacg gccgcgacct catcctgccc atcatcgagg
    13561 cggtggcctc gcaggtcttc atcccgctga cggtgggcgg cggcgtgcgc gaggtcgccg
    13621 acgtgcgccg gctgctcaac gcgggcgcgg acaaggtgag cttcaactcc gcggcggtcg
    13681 ccaacccgca ggtgatcgcc gaggcctcgg atcggtacgg cgcgcagtgc atcgtcgtgg
    13741 cgatcgacgc caagcgccgc agcgacgagg acgccgcgac gcgcggcccg ggctgggacg
    13801 tctacaccca cggcggccgc aagaacaccg gcctcgacgc ggtcgagtgg gcgcgcgaga
    13861 tggcgcgccg cggcgccggc gagatcctgc tcaccagcat ggaccgcgac ggcacgcgcg
    13921 ccggcttcga cctcgcgctg acccgcgccg tcgcggatgc cgtgccggtg ccggtgatcg
    13981 cctcgggcgg cgtcggcagc ctcgagcacc tggccgacgg cgtgcagcag ggcggcgccg
    14041 acgccgtgct cgcggcgagc atcttccact acggccagca caccgtcggc gaggccaagg
    14101 cgctgatggc ggcgcgcggc atcccggtgc gccgatgagg cctgcggcca agcgcgacgc
    14161 ggccccgggc gcacccgtcg cgggacggcc cggcgcgaag gcgaagccgc atcccggcgg
    14221 tccg
//
LOCUS       DASVLN010000238         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_226204, whole genome shotgun sequence.
ACCESSION   DASVLN010000238 DASVLN010000000
VERSION     DASVLN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226204"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VF453_05465"
     CDS             <1..1211
                     /locus_tag="VF453_05465"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX7687129.1"
                     /translation="CHHDDDPAALARDAAVAQALEADGRRLETAKDHVVFERSELLTG
                     AGKPYGVFTPYKNAWLQRLQAHPEAIAPHRSDSGAGALAALPAALASPWRGGAAVPEL
                     AEFGFEPPDDRLADAGLHPGSAGGEALLGDFLEHLDDYDAVRDFPARAGCSHLGVHLR
                     FGTLSIRRLAALALQRVEGGSRGAQTWLSELVWRDFFQQVLHHHPHVVGHAFRPEFEH
                     LRWERGAHADAAFEAWCAGRTGYPLVDAGMRQLAATGYMHNRLRMVTASFLAKDLGID
                     WRRGEAWFAQLLNDFELASNNGNWQWAASTGCDAQPWFRIFNPVTQSERFDPDGAFIR
                     RWVPELAKLPAKAIHAPWQAPADVLAAAGIELGRDYPRPVVDHARAREKTLARYGSRG
                     GARPRGETPT"
     gene            complement(1419..2447)
                     /locus_tag="VF453_05470"
     CDS             complement(1419..2447)
                     /locus_tag="VF453_05470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7687130.1"
                     /translation="MPEIIDHLASLTGLRDRDTLDVSLAYSIRDLLAPRRVAIYRLVG
                     DPGSERWLNRATLLADSPAATADPAWVDLNALPRADERPTWLECMTTTQVITSKDGDA
                     VTTLFPVVSDTGVVGVVELQTLRSVGPKTRRLVFGVLRIYRNVQSLLDYSERDTLTGL
                     LNRKIFDDSFYKVSSLPLLDAKLDDGERRHASGSRYWLGVIDIDHFKNVNDRFGHLIG
                     DEVLLLLSRIMRGCFRFSDQLYRFGGEEFVVLLLCSDEADAGAALERFRGVVEDYAFP
                     QAGRITVSVGFTAIEVGDTPAAAFERADRAVYHAKHNGRNQVSNHAELMRQGVIEDDK
                     RVSDVELF"
     gene            complement(2511..>4498)
                     /locus_tag="VF453_05475"
     CDS             complement(2511..>4498)
                     /locus_tag="VF453_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7687131.1"
                     /translation="VPAAEPPVEVVPLPAVAPLATHAPSVAIDWARFSASNPSRVVRA
                     VETAAANPATSMVRVRAPLLDRLVNQAGEVSITRSRMESDVGQMRGSLRELTENLDRL
                     RRQLRDIELQAESQIASRLEASRSSSVEFDPLEMDRFTRFQELTRMMAESVNDVATVQ
                     RSLQQTVQSAEDEMASQARLTRGLQEDLLRARMVPFDTLSERLYRVVRQAAKETSKQV
                     RLNVEGSSIELDRSVLDRMAGAFEHVLRNAVVHGIEDGDSRRVLDKDPVGTIDVALAQ
                     QGNEVRIQIRDDGRGLDLQRIEAKGRRNGLIAPDAHPTDAELANLIFTPGFSTAEEVT
                     ELAGRGVGMDAVRNDVTALGGRIETASAAGRGTAFTLLLPLTTAVTRVVQLRCGELTV
                     AIPASLVEVVRRAPVGEVEQAYATGQYRDGVNDVPFFWLGALLQASTHGTLVGRTTQV
                     VLVRSAEQRVALHVDQVMGNQEVVVKNLGPQLARLPGLAGMSLLATGEVVPIYNPVAL
                     AAFYGQRPGAEGAAPLVPEIVQEVEEVRAPLVLVVDDSLTVRRVTQRLLTREGFRVTV
                     AKDGLDALEQLEVERPTVMLTDIEMPRMDGFDLLRNVRHDPRWQDLPVIMITSRIAQK
                     HRDLAMQLGANHYLGKPYDEEQLIALIQQNVAKAMPA"
BASE COUNT          630 a         1611 c         1619 g          638 t
ORIGIN      
        1 actgccacca cgacgacgat cccgccgccc ttgcccgcga cgccgcggtg gcgcaggcgc
       61 tcgaggccga cgggcgccgg ctcgagacgg ccaaggacca tgtcgtcttc gagcgctccg
      121 agctgctgac gggcgccggc aagccctatg gcgtgttcac gccgtacaag aacgcgtggc
      181 tccagcggct gcaggcgcat cccgaggcga tcgcgccgca tcgcagcgac agcggcgccg
      241 gggcgctggc ggcgctcccg gcggcgctag cctcgccctg gcgcggcggg gccgcggtgc
      301 ccgagctcgc cgagttcggc ttcgagccgc ccgacgaccg cctcgccgac gccggcctgc
      361 accccggcag cgcgggcggc gaggcgctgc tgggcgactt cctggagcac ctcgatgact
      421 acgacgccgt ccgcgatttc cccgcccggg ccggctgcag ccacctgggc gtgcacctgc
      481 gcttcggcac gctgtcgatc cgccggctgg cggcgttggc gctgcagcgc gtcgagggcg
      541 gcagccgcgg ggcgcagacc tggctgtcgg agctggtctg gcgcgacttc ttccagcagg
      601 tgctgcacca ccatccgcat gtcgtcgggc atgcattccg gcccgagttc gagcacctgc
      661 gctgggaacg cggcgcccac gccgatgccg cgttcgaggc ctggtgcgcc ggccgcaccg
      721 gctatccgct ggtcgacgcg ggcatgcgcc agctggccgc gaccggctac atgcacaacc
      781 gcctgcgcat ggtgacggcc agcttcctcg cgaaggacct cggcatcgac tggcgccggg
      841 gcgaggcctg gttcgcgcaa ctgctcaacg acttcgagct cgcctccaac aacggcaact
      901 ggcagtgggc ggcgtccacc ggctgcgacg cccagccctg gttccgcatc ttcaacccgg
      961 tgacgcagag cgagcgcttc gaccccgacg gcgccttcat ccgccgctgg gtgcccgagc
     1021 tcgcgaagct gccggccaag gcgatccacg cgccctggca ggcgccggcg gacgtgctgg
     1081 ccgcggccgg catcgagctg ggacgcgact acccgcgccc cgtcgtcgac cacgcgcggg
     1141 cccgcgagaa gacgctcgcc cgctacggct cgcgcggcgg cgcccgaccc cgcggcgaga
     1201 ccccgacgta ggcgcgcagc gccgaggccc ttcccggcgc tgccgcttgg gcgacccaag
     1261 cgagacacgc gggagcggcg gatctggctt ggccagtccg ctcgcggccg gccccctcgg
     1321 ggagcgaagg cggcaaaggg ctcgcgcgag cgagaccccg ccgtagcggc tcgcggcatg
     1381 cttgccgcga gcccccggag cgccagcgac cgtgggggct agaacagttc cacgtcgctg
     1441 acgcgcttgt catcctcgat cacgccctgg cgcatcagct cggcatggtt gctgacctgg
     1501 ttgcggccgt tgtgcttggc gtgatagacg gcgcggtcgg cgcgctcgaa ggcggcggcg
     1561 ggcgtgtcgc cgacctcgat ggccgtgaag ccgacgctga cggtgatgcg gccggcctgg
     1621 gggaaggcgt agtcctcgac cacgccgcgg aagcgctcca gcgcggcgcc ggcgtccgcc
     1681 tcgtcgctgc acagcagcag cacgacgaac tcctcgccgc cgaaccggta gagctggtcc
     1741 gagaagcgga agcagccgcg catgatgcgc gacagcagca gcagcacctc gtcgccgatc
     1801 aggtggccga agcggtcgtt gacgttcttg aagtggtcga tgtcgatcac gcccagccag
     1861 tagcgcgagc ccgaggcatg gcggcgctcg ccgtcatcca gcttggcgtc gagcagcggc
     1921 agcgacgaga ccttgtagaa gctgtcgtcg aagatcttgc ggttcagcag cccggtgagc
     1981 gtgtcgcgct cgctgtagtc gagcagcgac tgcacgttgc ggtagatgcg cagcacgccg
     2041 aacacgaggc ggcgcgtctt cgggcccacc gagcgcaggg tctgcagctc gaccacgccg
     2101 accacgccgg tgtcggagac gaccgggaac agcgtcgtga ccgcatcgcc gtccttggag
     2161 gtgatgacct gcgtggtggt catgcactcg agccaggtcg ggcgctcgtc ggcgcgcggc
     2221 agcgcgttca ggtcgaccca ggcgggatcg gcggtggcgg ccggcgaatc ggccagcagc
     2281 gtcgcgcgat tgagccaccg ttccgagccc ggatcgccca ccagccggta gatcgccacg
     2341 cgccgcggcg cgagcagatc ccggatggaa taggccaggc tgacgtcgag cgtgtcgcgg
     2401 tcgcgcagcc cggtcagcga ggcaaggtgg tcgatgattt ccggcattgg aggatgagag
     2461 cgtgtcgacc ctgatatcgg aggctgccgg cgggactgta ggcagaacgt tcaggccggc
     2521 atcgccttgg cgacattctg ctggatcaac gcgatcaatt gctcttcatc atacggcttg
     2581 ccgaggtaat ggttcgcccc gagctgcatg gccaggtcgc ggtgcttctg ggcgatgcgc
     2641 gacgtgatca tgatgaccgg caggtcctgc cagcgcgggt cgtggcggac gttgcgcagc
     2701 aggtcgaagc cgtccatgcg cggcatctcg atgtcggtga gcatgacggt cgggcgctcg
     2761 acctcgagct gctcgagcgc atccaggccg tccttggcca ccgtgacgcg gaagccttcc
     2821 cgcgtgagca gccgctgcgt gacgcggcgc accgtgagcg agtcgtcgac cacgagcacc
     2881 agcggcgcgc gcacctcctc gacttcctgc acgatctccg gcaccagcgg cgcggcgccc
     2941 tcggcgcccg ggcgctggcc gtagaacgcg gcgagcgcga ccgggttgta gatcgggacg
     3001 acctcgccgg tggccagcag cgacatgccc gcgaggcccg gcaggcgcgc gagctgcggc
     3061 ccgaggttct tcacgaccac ttcctggttg cccatgacct ggtcgacgtg cagggcgacc
     3121 cgctgctcgg cgcttcgcac cagcacgacc tgcgtcgtcc ggcccaccag cgtgccgtgg
     3181 gtcgacgcct gcagcagcgc gccgagccag aagaacggga cgtcgttgac gccgtcgcgg
     3241 tactggccgg tcgcataggc ctgctcgacc tcgcccaccg gcgcgcgccg cacgacctcg
     3301 accagcgagg ccgggatcgc caccgtcagc tcgccgcagc gcagctgcac gacgcgcgtc
     3361 acggcggtcg tcagcggcag cagcagcgtg aaggccgtgc cgcggccggc ggccgatgcc
     3421 gtctcgatgc ggccgcccag cgcggtgacg tcgttgcgca cggcgtccat gcccacgccg
     3481 cggccggcga gctcggtcac ctcctcggcg gtcgagaagc cgggcgtgaa gatcaggttc
     3541 gcgagttcgg cgtccgtcgg atgcgcgtcc ggcgcgatca acccgttgcg ccggcccttg
     3601 gcctcgatgc gctgcaggtc gaggccgcgg ccgtcgtcgc ggatctggat ccgcacctcg
     3661 ttgccctgct gcgcgagcgc gacgtcgatg gtgccgaccg gatccttgtc cagcacgcgg
     3721 cggctgtcgc cgtcctcgat gccgtgcacg acggcattgc gcagcacgtg ctcgaaggcg
     3781 ccggccatgc gatccaggac acttcgatcc agctcgatcg agctgccttc cacgttcagg
     3841 cgaacctgct tggacgtctc cttggcagcc tggcgcacga cgcggtacag gcgctcggac
     3901 agcgtgtcga acggcaccat gcgggcgcgc agcaggtctt cctgcagccc gcgcgtcagg
     3961 cgcgcctggg aggccatctc gtcttcggcc gactgcaccg tctgctgcag gctgcgctgc
     4021 acggtggcca cgtcgttgac cgactcggcc atcatccgcg tcaattcctg gaagcgcgtg
     4081 aaccggtcca tctcgagcgg gtcgaactcg accgacgacg agcggctcgc ctccaggcgc
     4141 gaggcgatct gcgactcggc ctgcagctcg atgtcgcgca gctgccggcg caggcggtcc
     4201 aggttttccg tcagttcgcg cagcgagccg cgcatctggc cgacgtcgct ctccatgcgc
     4261 gagcgcgtga tggagacttc gccggcctgg ttgacgaggc ggtcgagcag cggggcgcgc
     4321 acgcgcacca tcgacgtcgc cgggttcgcg gcggccgtct cgacggcccg cacgacgcgc
     4381 gacgggttgc tggcgctgaa ccgggcccag tcgatcgcca ccgacggcgc gtgggtcgcc
     4441 agcggcgcga ccgcgggcag gggcacgacc tcgacgggag gctccgcggc gggcacgg
//
LOCUS       DASVLN010000239         6430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_387369, whole genome shotgun sequence.
ACCESSION   DASVLN010000239 DASVLN010000000
VERSION     DASVLN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387369"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..969)
                     /locus_tag="VF453_05480"
     CDS             complement(70..969)
                     /locus_tag="VF453_05480"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF028064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pesticin C-terminus-like muramidase"
                     /protein_id="HEX7687132.1"
                     /translation="MADTRVPGVQGQPASPIAVPARTPGPLGRNDAADPNTTARLGDT
                     PGTSGVNDSHAPSLPAATAAGGPPAAKLPDGTAIAPGADGKAAALVCPAPLAKRADGG
                     KVDVDWAFIREREGDKLDGYVPDASGSSSGVTIATGIDLGQRSGSDIDKLDITDDLKK
                     KLKPYCLKTGKDATDLLAKSPLSITADEASALTKAVKGPMLDALIKEYDAAVDKANAA
                     DHCARVHFAELPGSVQTALASAQFQYNSLSSRTQNYWKQVTQQKWDEASKNLKNFGDR
                     YKTRRKLEAGLIDDAIAAAVPPK"
     gene            complement(1088..1897)
                     /locus_tag="VF453_05485"
     CDS             complement(1088..1897)
                     /locus_tag="VF453_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7687133.1"
                     /translation="MGFRLVTLNLNGIRSAHNKGFLQWAETMKADCMGVQEVKAQHEH
                     VHEVFDTVDGMAGRFHYALKKGYSGVGLYSRVAPTEVLTGFGEQEFDDEGRYVEMRFD
                     KPGAGLKHPFEKLSIISCYFPSGSSGEERQAAKFRFLDVIYPHLMALKAQREFILVGD
                     INIAHKEIDLKNWKGNQKNSGFLPEERAWMTKLLTDGGLVDVFRTLNDRAEQYTWWSN
                     RGQAWAKNVGWRLDYHLATPGVAALARREHIYLEQRFSDHAPLAIDYEFTL"
     gene            1912..2592
                     /gene="pyrE"
                     /locus_tag="VF453_05490"
     CDS             1912..2592
                     /gene="pyrE"
                     /locus_tag="VF453_05490"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7687134.1"
                     /translation="MTDIAATDALAQDFVRFSMEAGVLRFGDFTTKAGRQSPYFFNAG
                     LFDDGDKLMRLGEFYARRILASGIAFDMLFGPAYKGITLAAAVAIGLTRHGRNVGYAY
                     NRKEAKAHGEGGTLVGAPVRGRVLIIDDVITDGASKREAADLIRAAGAEPVGVAIMMD
                     RQERARDEGEDRRSGVQYVEQTLGLRVVSVATLADLMAMLSTTGDPALAANAARVAAY
                     RARYGIDQ"
     gene            2618..2995
                     /locus_tag="VF453_05495"
     CDS             2618..2995
                     /locus_tag="VF453_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687135.1"
                     /translation="MTALDRPLRRAGRAAILLAMGAACAPAGAMAVKYQCIGYRPLTA
                     ELTPRDGHVQFEGHDWRLRRVAAGGGQARYVGREVTITAKQRELTLVHGDETLRCVLL
                     SDAVQEFAPHPPGAASAASAPQR"
     gene            complement(3042..4490)
                     /gene="gatB"
                     /locus_tag="VF453_05500"
     CDS             complement(3042..4490)
                     /gene="gatB"
                     /locus_tag="VF453_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX7687136.1"
                     /translation="MSTLIRGYEVVIGFETHTQLSTVSKIFSGASTQFGAAPNTQASA
                     VDLALPGTLPVLNRGAVERAIKFGLAVGATVAPLSIFARKNYFYPDLPKGYQISQYEI
                     PVVQGGAVSFMLDGQPRTVKLTRAHLEEDAGKSVHEGEWSGIDLNRAGTPLLEIVTEP
                     DMRSTTEAVEYAKALHALVVWLGICDGNMQEGSFRCDANVSVRKPGQPFGTRREIKNL
                     NSFRFLQQAIDYEINWQIDEIEDGRKIQQATVLFDPDTGETRMMRTKEDAQDYRYFPD
                     PDLPPLVISRAWVDEVRAAMPELPGVMAARFVRDDGLPEYDAAMMTQSLAVARYYEAA
                     RDAGAPAKLVANWVMGELSRRLNAEGRDIAESPVEAPLLATLIQRIQDGTVSNNAARK
                     VFDALWTREKGTAGNVDETIDRLDLRTSNDAGALEAIIDGVLAANQKSVDEFRAGKDK
                     AFQALVGQAMKASKGKANPAQVNELLKKKLGV"
     gene            complement(4487..5416)
                     /locus_tag="VF453_05505"
     CDS             complement(4487..5416)
                     /locus_tag="VF453_05505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX7687137.1"
                     /translation="MPAPVRGAVVEGDCLDVLRAVPAERVDLIVTSPPYADQRASTYG
                     GVHPDDYVDWFLPRAQQFARVLKASGSLVVNIKEKVVGGERHTYVLELILAMRKQGWL
                     WTEEYVWHKRNCFPGKWPNRFRDAWERCLHFTRERRFKMNQDAVMVPMGDWAQSRLKT
                     LGANDVVRFDSRVGNAFGKNVANWQGRERAYPTNVLHLATECGNKGHSAAFPQALPRW
                     FIELFTDPGDVVLDPFAGSGTTLAAAIALGRHAQGVELNPDYARRCEAAVTGALQAAE
                     ALRRAAAPDPELPSSGRRDDLATPPIPKDRVPS"
     gene            complement(5465..>6430)
                     /locus_tag="VF453_05510"
     CDS             complement(5465..>6430)
                     /locus_tag="VF453_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX7687138.1"
                     /translation="GGSIRQPASFTGTTGIKPTYGVCSRFGMIAFASSLDQAGPLARS
                     AEDCALLLSAMSGFDERDATSVQRPPQDFHAQMLAPRPGANAARPLAGLRIGLPKEFF
                     PAALAADVDGRVRAALAEMEKLGATLVDVSLPRTELSIPVYYIIAPAEASSNLSRYDG
                     VRYGHRTASHGDLLDMYKKSRSEAFGPEVKRRIMIGTYVLSHGYYDAYYLQAQKLRRM
                     IADDFQAAFKSCDLIAGPVAPTVAWTLGERNDDPVKNYLADIFTLPGSLAGLPGMSVP
                     AGLGDGGMPVGLQLLGNYFAEGTLLHAAHALQQATDFHTLAPAAR"
BASE COUNT          864 a         2199 c         2343 g         1024 t
ORIGIN      
        1 cgacggtcgc gaacgcgctc cacgcgagcg ctgccacggc caggcggagg cgggatgcgc
       61 ggcgcgccgt catttcggag gcaccgcggc cgcgatcgcg tcgtcgatca gccccgcctc
      121 gagcttgcgc cgcgtcttgt agcggtcgcc gaagttcttc aggttcttgc tcgcctcgtc
      181 ccatttctgc tgcgtcacct gcttccagta gttctgcgtc cgcgaggaca gcgagttgta
      241 ctggaactgc gccgacgcga gcgccgtctg gacgctgccc ggaagctccg cgaaatgcac
      301 gcgcgcgcag tgatcggcgg cgttggcctt gtcgacggcg gcgtcgtact ccttgatcag
      361 cgcatccagc atcggcccct tcacggcctt ggtcagcgcg ctggcctcgt cggcggtgat
      421 gctcagcggg gacttcgcga gcaggtcggt cgcgtccttg ccggtcttca ggcagtaggg
      481 cttcagcttc ttcttcaggt cgtcggtgat gtcgagcttg tcgatgtccg acccgctgcg
      541 ctgcccgagg tcgatgcccg tggcgatcgt cacgccgctc gagctgcccg acgcgtcggg
      601 cacgtagccg tcgagcttgt cgccctcgcg ctcgcggatg aaggcccaat cgacgtcgac
      661 cttgccgccg tcggcgcgct tggccagcgg cgcagggcag acgagcgccg ccgccttgcc
      721 gtcggcgccg ggggcgatgg ccgtgccgtc aggcagcttg gctgccggcg gaccgccggc
      781 ggcggtcgcg gccggcaggc tgggcgcatg cgaatcgttg acgccggacg tccccggcgt
      841 gtcgccgagg cgcgcggtgg tgttcgggtc ggccgcatcg ttgcgcccga gcggccccgg
      901 cgtgcgggcg gggacggcga tcggcgaggc gggctggcct tggacgccgg gaacgcgtgt
      961 atcagcgatg acggactccc ctcgggatgg gcaatgcggg cgatactacc ccgaggcgaa
     1021 cccgtcggcg cccgcgtcgt tccgggccgc tgccgtcagg ggcggcgcgc gcggtggccg
     1081 caggcgctca gagcgtgaac tcgtagtcga tcgccagcgg cgcgtggtcg ctgaagcgct
     1141 gctcgaggta gatgtgctcc ctgcgcgcga gcgccgcgac gccgggcgtg gcgaggtggt
     1201 agtcgaggcg ccagcccacg ttcttcgccc aggcctggcc gcggttgctc caccacgtgt
     1261 actgctcggc gcggtcgttg agcgtgcgga agacgtcgac gaggccgccg tcggtcagca
     1321 gcttcgtcat ccaggcgcgc tcctcgggca ggaagccgga gttcttctgg ttgcccttcc
     1381 agttcttgag gtcgatttcc ttgtgggcga tgttgatgtc gcccacgagg atgaactcgc
     1441 gctgcgcctt cagcgccatc aggtgcgggt agatgacgtc gaggaagcgg aacttggcgg
     1501 cctggcgctc ctcgccggac gagccgctcg ggaagtagca gctgatgatg ctcagcttct
     1561 cgaaaggatg cttgagcccc gcgccgggct tgtcgaagcg catctcgacg tagcggccct
     1621 cgtcgtcgaa ctcctgctcg ccgaaaccgg tgagcacctc ggtcggcgcg acgcgactgt
     1681 acaggcccac gccggagtag cccttcttca gtgcgtagtg gaagcggccg gccatgccgt
     1741 cgacggtgtc gaagacctcg tgcacgtgct cgtgctgcgc cttcacctcc tgcacgccca
     1801 tacaatcggc cttcatcgtc tcggcccatt gcaggaagcc cttgttgtgg gcggaccgga
     1861 tgccgttcag gttcagggtg acgaggcgaa atcccaaggg tgatcctcca aatgaccgac
     1921 attgccgcca ccgacgcgct cgcgcaggac ttcgtccgct tctcgatgga ggccggcgtg
     1981 ctgcggttcg gcgacttcac gacgaaggcc ggacggcagt cgccgtattt cttcaacgcc
     2041 gggctgttcg acgacggcga caagctcatg cggctgggcg aattctatgc acgccgcatc
     2101 ctcgcgtccg gcatcgcgtt cgacatgctg ttcgggccgg cctacaaggg catcacgctg
     2161 gcggcggccg tggccatcgg cctgacgcgg cacggccgca acgtcggcta cgcctacaac
     2221 cgcaaggagg ccaaggcgca cggcgaaggc ggcacgctgg tcggcgcgcc ggtgcgcggc
     2281 cgggtgctga tcatcgacga cgtgatcacc gacggcgcct ccaagcgcga ggccgccgac
     2341 ctgatccgcg ccgcgggcgc cgagcccgtg ggcgtcgcga tcatgatgga ccgccaggaa
     2401 cgcgcccgcg acgagggcga ggatcgccga tccggcgtgc agtacgtcga gcagacgctg
     2461 ggcctgcgcg tggtctcggt cgcgacgctg gcagacctga tggcgatgct gtcgacgacc
     2521 ggcgacccgg cgctggccgc caacgccgcg cgcgtcgccg cctaccgcgc ccgctacggg
     2581 atcgaccagt aaccggccac agccaggagc agcacccatg accgccctcg accgacccct
     2641 ccgtcgcgcg gggcgcgcgg cgatcctgct ggccatgggc gccgcatgcg cgcccgcggg
     2701 cgcgatggcc gtcaagtacc agtgcatcgg ctaccggccg ctcaccgccg agctcacgcc
     2761 gcgcgacggc cacgtgcagt tcgagggcca cgactggcgc ctgcgccgcg tcgcggcggg
     2821 cggcggccag gcccgctacg tcggccgcga ggtcacgatc acggcgaagc agcgcgagct
     2881 cacgctggtg cacggcgacg agacgctgcg ctgcgtgctg ctgtccgacg cggtgcagga
     2941 attcgcgccg catccgccag gcgcggcgtc ggcggcctcg gcgccgcagc gctgacacgg
     3001 cgcgaggggc cgacgcggcg acgacgcgcg tcccgtgcgc ttcagacccc gagcttcttc
     3061 ttcagcagct cgttgacctg cgccgggttg gccttgccct tgctggcctt catcgcctgg
     3121 ccgacgaggg cctggaaggc cttgtccttg ccggcgcgga actcgtcgac cgacttctgg
     3181 ttcgcggcca gcacgccgtc gatgatcgcc tcgagcgcac cggcgtcgtt gctcgtgcgc
     3241 aggtcgagcc ggtcgatggt ctcgtcgacg ttgcccgccg tgcccttctc gcgcgtccac
     3301 agcgcgtcga agaccttgcg cgcggcgttg ttcgagacgg tgccgtcctg gatccgctgg
     3361 atcagcgtgg cgagcagcgg cgcctccacc ggactctcgg cgatgtcgcg gccctcggcg
     3421 ttcagccgcc gcgagagttc gcccatcacc cagttggcga ccagcttggc cggcgcgccg
     3481 gcgtcgcgcg cggcctcgta gtagcgcgcg accgcgagcg actgcgtcat catcgcggcg
     3541 tcgtactcgg gcaggccgtc gtcgcgcacg aagcgggcgg ccatcacgcc gggcagctcc
     3601 ggcatcgccg cgcgcacctc gtcgacccac gcgcgcgaga tcacgagcgg cggcaggtcg
     3661 gggtcgggga agtagcggta gtcctgcgcg tcctccttgg tgcgcatcat gcgcgtctcg
     3721 cccgtgtcgg ggtcgaacag cacggtggcc tgctggatct tgcggccgtc ctcgatctcg
     3781 tcgatctgcc agttgatctc gtagtcgatc gcctgctgca ggaagcggaa gctgttgagg
     3841 ttcttgatct cgcgccgcgt gccgaagggt tggcccggct tgcgcaccga gacgttggcg
     3901 tcgcagcgaa acgagccctc ctgcatgttg ccgtcgcaga tgccgagcca gacgaccagc
     3961 gcgtgcagcg ccttcgcgta ctcgacggcc tcggtggtgg agcgcatgtc gggctcggtg
     4021 acgatctcca gcagcggcgt gccggcgcgg ttcaggtcga tgcccgacca ctcgccctcg
     4081 tggaccgact tgccggcgtc ctcctcgagg tgggcgcgcg tgagcttcac cgtgcgcggc
     4141 tggccgtcca gcatgaacga gaccgcgccg ccctggacga ccgggatctc gtactggctg
     4201 atctggtagc ccttgggcag gtcggggtag aaatagttct tgcgggcgaa gatggacagc
     4261 ggcgccacgg tcgcgccgac cgcgaggccg aacttgatcg cgcgctcgac cgcgccgcga
     4321 ttgagcaccg gcagcgtgcc gggcagcgcg aggtcgaccg ccgaggcctg cgtgttgggc
     4381 gcggcgccga actgcgtcga cgcgcccgag aagatcttgc tgacggtgga gagctgcgtg
     4441 tgggtctcga agccgatcac gacttcgtag ccgcggatga gggtgctcat gatggaacgc
     4501 ggtccttggg aatcgggggc gtcgcgaggt cgtcgcggcg tccggaggaa gggagttcgg
     4561 gatcgggcgc ggcggcgcgg cgcagcgcct cggcggcctg cagcgcaccg gtgacggcgg
     4621 cctcgcagcg gcgcgcgtag tcgggattca gctcgacgcc ctgcgcgtgg cgcccgagcg
     4681 cgatggccgc cgccagcgtg gtgccggagc cggcgaacgg atcgagcacg acgtcgccgg
     4741 ggtcggtgaa cagctcgatg aaccagcgcg gcagcgcttg cgggaaggcc gcgctgtggc
     4801 ccttgttgcc gcactcggtc gcgaggtgca ggacgttcgt gggataggcg cgctcgcggc
     4861 cctgccagtt ggcgacgttc ttgccgaacg cgttgcccac gcgcgaatcg aagcgcacga
     4921 cgtcgttggc gcccagcgtc ttcaggcgcg actgcgccca gtcgcccatc ggcaccatca
     4981 cggcgtcctg gttcatcttg aagcgcctct cgcgcgtgaa gtgcaggcag cgctcccacg
     5041 cgtcgcggaa gcggttgggc cacttgccgg gaaagcagtt gcgcttgtgc cagacgtatt
     5101 cctcggtcca cagccagccc tgcttcctca tggccaggat cagctcgagg acgtaggtgt
     5161 ggcgctcgcc gccgacgacc ttttccttga tgttgaccac cagcgagccg gaggccttca
     5221 gcacgcgcgc gaactgctgc gcgcgcggca ggaaccagtc gacgtagtcg tccgggtgca
     5281 cgccgccgta ggtcgaggcg cgctggtcgg cgtagggcgg cgaggtgacg atgaggtcga
     5341 cccgctcggc gggcaccgcg cgcaagacgt ccaggcagtc gccctcgacc acggcgccgc
     5401 ggacgggcgc gggcatggcg gcgaagcgat cggccggggc gttcatgtcg gctgcaacga
     5461 ccggtcagcg cgcggcgggc gccagcgtgt ggaaatccgt cgcctgttgc aacgcgtgcg
     5521 cggcgtgcag cagcgtgccc tcggcgaagt agttgcccag cagctgcagg cccaccggca
     5581 tgccgccgtc tcccagcccc gcgggcacgc tcatgcccgg caggccggcg agcgagccgg
     5641 gcagcgtgaa gatgtcggcg aggtagttct tcaccgggtc gtcgttgcgc tcgccgaggg
     5701 tccacgccac cgtcggcgcc accgggccgg cgatcaggtc gcaggacttg aaggcggcct
     5761 ggaagtcgtc ggcgatcatt cgacgcagct tctgtgcctg caggtagtag gcgtcgtagt
     5821 agccgtggct cagcacgtag gtgccgatca tgatgcggcg cttgacctcg gggccgaagg
     5881 cctcgctgcg cgacttcttg tacatgtcca gcaggtcgcc gtggctggcc gtgcgatggc
     5941 cgtagcgcac gccgtcgtag cggctcaggt tcgagctcgc ctcggccggc gcgatgatgt
     6001 agtagaccgg gatcgacagc tcggtgcgcg gcagcgagac gtcgaccagc gtcgcgccca
     6061 gcttctccat ctcggcgagc gccgcgcgca cgcggccgtc gacgtcggcg gccagcgccg
     6121 ccgggaagaa ctccttcggc agcccgatgc gcaggcccgc gagcggcctt gccgcgttcg
     6181 cgcccgggcg cggcgcgagc atctgcgcgt ggaagtcctg cggcgggcgc tgcacgctgg
     6241 tggcgtcgcg ctcgtcgaag ccgctcatcg ccgacagcag cagggcgcag tcctcggccg
     6301 agcgcgcgag cggccccgcc tggtcgaggc tggacgcgaa cgcgatcatg ccgaagcgcg
     6361 agcacacgcc gtaggtgggc ttgatgcccg tcgtgccggt gaagctggcg ggctggcgga
     6421 tcgagccgcc
//
LOCUS       DASVLN010000240         4750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_65341, whole genome shotgun sequence.
ACCESSION   DASVLN010000240 DASVLN010000000
VERSION     DASVLN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65341"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VF453_05515"
     CDS             complement(<1..611)
                     /locus_tag="VF453_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX7687139.1"
                     /translation="MSDRGRRVWLFDLDNTLHDAGAWVFARMNDTMRTYVVDTLGVTP
                     AEADALRERYWRRYGSTMLGLVRHHGVRPAHFLLETHRFPGLEERVTGHRHDLAAVAR
                     LRGRRIVLTNAPRAYALRVLGALGALRLFDEVLSIEDMTMFGHWRPKPDRRMLRAVAV
                     RLGVPPARCTLVEDSLENVLAARRVGMRAVWMQRFARRGAHAGP"
     gene            complement(608..1540)
                     /gene="argB"
                     /locus_tag="VF453_05520"
     CDS             complement(608..1540)
                     /gene="argB"
                     /locus_tag="VF453_05520"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7687140.1"
                     /translation="MTTPTPSSPAASHPDADLSHIAPRDKAEILAQALPYIRRFHGKT
                     MVIKYGGNAMTEPALQQDFAEDVVLLKLVGINPVVVHGGGPQIDTALSKLGKKGTFIQ
                     GMRVTDQETMDVVEWVLGGEVQQDIVGLINGAGGKAVGLTGFDGAMIRARKLKMKDKD
                     DPSIEHDVGQVGDIVSVDPSVVKALQDDQFIPVVSPIGFGEGNQSFNINADVVAGKLA
                     EVLHAEKLVLLTNTPGVLDKAGKLLTDLSAREIDALFADGTISGGMLPKIASALDAAR
                     SGVNAVHIIDGRVPHAMLLEILTDQAYGTMIRAR"
     gene            1646..1936
                     /locus_tag="VF453_05525"
     CDS             1646..1936
                     /locus_tag="VF453_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HEX7687141.1"
                     /translation="MRRLAPTLAALALAAASAAAFAQAVVAGEVKKVDKPAGRITLKH
                     AEIKLYDMPPLTGAYKVQDPSMLDKVQVGDHVKFTLDRVNSVYTITKIDVMK"
     gene            complement(1965..3974)
                     /locus_tag="VF453_05530"
     CDS             complement(1965..3974)
                     /locus_tag="VF453_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7687142.1"
                     /translation="MQYFVTGATGFIGRRLVKTLLARRGATVYFLVRPGSEGKVADLL
                     AYWGVSKTRAIPVSGDLTSRKLGVAADQVKALKGQIDHAYHLAAVYDLGADEEQQVAV
                     NIEGTRNAVEFAKAIDARHFHHVSSIAAAGLYEGVFREDMFDEAENLDHPYYATKHES
                     EKIVRRECKVPWTVYRPAVVVGDSRTGEMDKIDGPYYFFKLIQRMRQILPPWMPGVGL
                     EGGRVNIVPVDFVVAALDHISHQQGGRSGGCYHLTDPEGLRVGDVLGIFAKAAHAPKM
                     NLFVNAALLGFVPKGVKKSLMALAPVRRVRNAVMKDLGLPEDILSFINYPTRFDNREA
                     TAALKGSGIECPPLRDYAWRLWDYWERHLDPALKIDHSLKGTVAGKVVLVTGGSSGIG
                     LAAACKFAEAGAITIICARDEGKLAEAKAEILAYARQGGRADARVETYSVDIADEAGC
                     KAFVGWLTEQFGGVDFLVNNAGRSIRRAIESSYDRFHDYERTMQLNYFGCLRVTMGLL
                     PGMVAKRKGHVVNISSIGVLTNAPRFSAYVASKAALDAWTRCASSEYADVGVTFTTIN
                     MPLVRTPMIAPTKIYNNVPTLSPEEAADMIAQACVEKPVRIATRLGVFGEVLHAFVPR
                     VAQIVSNTTFRMFPDSEAAAGDKGGKPKLSAEAVALQQMMRGIHF"
     gene            4162..>4750
                     /locus_tag="VF453_05535"
     CDS             4162..>4750
                     /locus_tag="VF453_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX7687143.1"
                     /translation="MVKKLSKIAAKKASSPAGMFDSALASAVKDSAQQIWLAGMGAFA
                     KAQAEGGKVFDALVKEGVSLQRKTQAVAEEKLGDVTGKMSSMASEVTSKAGASWDKLE
                     NIFEERTAKALGKLGVPTAKDVAELSRRVEELSAAVARLSKSPKAAPASAKTPAAKAP
                     AKKAAAKRAPAKKATTANGAAKPAAKRAAAKRAAAS"
BASE COUNT          715 a         1627 c         1677 g          731 t
ORIGIN      
        1 ggccccgcat gcgcgccgcg ccgggcgaag cgctgcatcc acaccgcccg catgccgacg
       61 cgacgtgccg ccagcacgtt ctccagcgaa tcctcgacca gggtgcagcg cgccggtggc
      121 actcccaggc gaaccgcgac cgcgcgcagc atgcgccggt cgggcttggg ccgccagtgg
      181 ccgaacatcg tcatgtcctc gatggagagg acttcgtcga acagccgcag cgcgcccagc
      241 gccccgagca cccgcagcgc gtaggcgcgc ggcgcgttgg tgagcacgat gcgccggccg
      301 cgcaggcgcg cgacggcggc gaggtcgtgg cgatggcccg tgacacgctc ctcgagcccg
      361 ggaaagcggt gcgtctcgag caggaagtgc gccggccgca cgccgtggtg gcggacgagg
      421 cccagcatcg tcgagccgta gcggcgccag tagcgctcgc gcagcgcgtc ggcctcggcg
      481 ggcgtgacgc ccagcgtgtc gacgacgtag gtgcgcatgg tgtcgttcat ccgcgcgaac
      541 acccacgcgc cggcgtcgtg cagcgtgttg tcgaggtcga agagccagac gcgccggccg
      601 cggtcgctca ccgggcgcgg atcatcgtgc cgtaggcctg gtcggtcagg atctccagca
      661 gcatggcgtg cggcacgcgg ccgtcgatga tgtgcacggc gttgaccccc gagcgcgcgg
      721 cgtccagcgc cgaggcgatc ttgggcagca tgccgcccga gatcgtgccg tcggcgaaca
      781 gggcgtcgat ctcgcgcgcc gacaggtccg tcagcagctt gccggccttg tccagcacgc
      841 cgggcgtgtt ggtcagcagc accagcttct cggcgtggag cacctcggcc agcttgccgg
      901 cgacgacgtc ggcgttgatg ttgaagctct ggttgccctc gccgaagccg atcgggctga
      961 cgaccgggat gaactggtcg tcctgcagcg ccttcacgac ggacgggtcc accgagacga
     1021 tgtcgccgac ctgacccacg tcgtgctcga tcgacgggtc gtccttgtcc ttcatcttga
     1081 gcttgcgcgc gcggatcatc gcgccgtcga agcccgtcag gccgaccgcc ttgccgccgg
     1141 cgccgttgat gaggccgacg atgtcctgct gcacctcgcc gccgagcacc cattcgacga
     1201 cgtccatcgt ctcctggtcg gtcacgcgca tgccctggat gaaggtgccc ttcttgccga
     1261 gcttggacag cgccgtgtcg atctgcgggc cgcccccgtg cacgaccacc gggttgatgc
     1321 cgaccagctt cagcagcacc acgtcctcgg cgaagtcctg ctgcagcgcc ggctccgtca
     1381 tcgcgttgcc gccgtacttg atgaccatcg tcttgccgtg gaacctgcgg atgtacggca
     1441 gcgcctgggc gaggatctcg gccttgtcgc gcggggcgat gtgcgacagg tcggcgtcgg
     1501 gatgtgacgc ggcggggctg gagggcgtgg gcgtggtcat gcgcggattc tagggacctt
     1561 tccgcgaccg cagcatgtcg cctcagggcc gcgtgtcggc cggaaacggg ccgcggacgt
     1621 tggaggagta cagtcccttc gcatcatgag acgcctcgcc cccaccctcg ccgccctcgc
     1681 cctcgccgcc gccagcgcgg ccgccttcgc ccaggcggtc gtcgccggcg aggtcaagaa
     1741 ggtggacaag cctgccggcc gcatcacgct caagcatgcc gagatcaagc tctacgacat
     1801 gcctccgctg accggcgcct acaaggtgca ggatccgtcc atgctcgaca aggtgcaggt
     1861 cggcgaccac gtgaaattca cgctcgaccg cgtgaactcg gtctacacca tcaccaagat
     1921 cgacgtcatg aagtgacggc ggcgcccgcg ggcgccgcat cgcctcagaa gtggatcccg
     1981 cgcatcatct gctgcagcgc gaccgcttcg gccgagagct tgggcttgcc gcccttgtcg
     2041 cccgccgcgg cctcggagtc ggggaacatg cggaacgtcg tgttgctgac gatctgcgcg
     2101 acgcgcggca cgaaggcgtg cagcacctcg ccgaacacgc ccaggcgcgt cgcgatgcgc
     2161 accggcttct cgacgcaggc ctgcgcgatc atgtcggcgg cctcctccgg cgagagcgtg
     2221 ggcacgttgt tgtagatctt cgtgggcgcg atcatcggcg tgcgcacgag cggcatgttg
     2281 atcgtcgtga aggtgacgcc gacgtcggcg tactcgctcg acgcgcagcg cgtccaggcg
     2341 tccagcgcgg ccttgctcgc gacataggcc gagaagcgcg gcgcgttggt cagcacgccg
     2401 atcgagctga tgttgacgac gtggcccttg cgcttggcca ccatgccggg cagcaggccc
     2461 atcgtgacgc gcaggcagcc gaagtagttc agctgcatcg tgcgctcgta gtcgtggaag
     2521 cggtcgtagc tcgactcgat cgcgcgccgg atcgagcggc cggcgttgtt gaccaggaag
     2581 tcgacgccgc cgaactgctc ggtcagccag ccgacgaagg ccttgcagcc ggcctcgtcg
     2641 gcgatgtcca cggagtaggt ctccacgcgc gcgtccgcgc ggccgccttg cctggcgtag
     2701 gcgaggatct cggccttggc ctcggcgagc ttgccctcgt cgcgggcgca gatgatggtg
     2761 atcgcgcccg cctcggcgaa cttgcaggcc gcggccaggc cgatgccgga ggagccgccc
     2821 gtcaccagca cgaccttgcc ggcgacggtg cccttgaggc tgtggtcgat cttcagcgcc
     2881 gggtcgaggt ggcgctccca gtagtcccac aggcgccacg cgtagtcgcg cagcggcggg
     2941 cactcgatgc cggagccctt gagcgcggcg gtggcctcgc ggttgtcgaa gcgggtcggg
     3001 tagttgatga agctcaggat gtcctcgggc aggccgaggt ccttcatcac cgcgttgcgc
     3061 acccggcgca ccggcgcgag cgccatcagg ctcttcttca cgcccttcgg gacgaagccg
     3121 agcagcgcgg cgttcacgaa caggttcatc ttcggcgcat gcgcggcttt cgcgaagatg
     3181 cccagcacgt cgcccacgcg caggccttcc gggtcggtga ggtggtagca gccgccgctg
     3241 cgcccgcctt gctggtggct gatgtggtcg agcgccgcga cgacgaagtc caccggcacg
     3301 atgttcacgc gcccgccctc gagcccgacg ccgggcatcc agggcggcag gatctggcgc
     3361 atgcgctgga tcagcttgaa gaagtagtac gggccgtcga tcttgtccat ctcgcccgtg
     3421 cgcgagtcgc cgacgaccac ggcgggccga tagaccgtcc agggcacctt gcactcgcgg
     3481 cggacgatct tctcggactc gtgcttggtc gcgtagtacg ggtggtcgag gttctcggcc
     3541 tcgtcgaaca tgtcctcgcg gaagacgcct tcgtagaggc ccgccgcggc gatggacgag
     3601 acgtggtgga agtgcctggc gtcgatcgcc ttggcgaact cgacggcgtt gcgcgtgccc
     3661 tcgatgttga cggcgacctg ctgctcctcg tcggccccga ggtcgtagac ggcggcgagg
     3721 tggtaggcgt ggtcgatctg gcccttgagc gccttcacct ggtcggccgc gacgcccagc
     3781 ttcctgctcg tgaggtcgcc gctgaccggg atcgcgcggg tcttcgagac gccccagtag
     3841 gcgaggaggt cggcgacctt gccctccgag cccggtcgca ccaggaaata caccgtggcg
     3901 ccgcggcgcg cgagcagggt cttgacgagt cgcctgccga tgaagccggt cgcgccggtc
     3961 acgaaatact gcattcggtg tctcctctga aggtcggtgg ttcgggcccc ggggccagga
     4021 ggtcgattgt gggacagccg cgcggcgcgg cgcacgcgtg tttgtcccgt gcaacgccat
     4081 agagcgctga ccctacggaa ttaggctgcg cgccccatcg gcaatgccta tatttcgagt
     4141 cttcaaacca aaggaaatcc catggtcaag aagctttcca agatcgccgc gaagaaggcc
     4201 tcctcgccgg ccggcatgtt cgacagcgcg ctcgcgagcg ccgtcaagga ttcggcccag
     4261 cagatctggc tggccggcat gggcgcgttc gccaaggcgc aggccgaggg cggcaaggtg
     4321 ttcgacgcgc tggtcaagga aggcgtgagc ctgcagcgca agacgcaggc ggtcgccgag
     4381 gagaagctgg gcgacgtcac cggcaagatg agctcgatgg cctccgaggt cacgagcaag
     4441 gccggcgcgt cgtgggacaa gctcgagaac atcttcgagg agcgcaccgc caaggcgctg
     4501 ggcaagctgg gcgtgccgac cgccaaggac gtcgccgagc tgtcgcgccg cgtcgaggag
     4561 ctgagcgccg ccgtggcccg cctctcgaag tcgccgaagg ccgcgccggc ctccgcgaag
     4621 accccggcgg ccaaggcccc ggcgaagaag gccgccgcca agcgcgcgcc cgcgaagaag
     4681 gcgacgacgg ccaacggcgc ggccaagccc gccgccaagc gcgcggccgc gaagagggca
     4741 gccgcctcga
//
LOCUS       DASVLN010000241         6189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_281201, whole genome shotgun sequence.
ACCESSION   DASVLN010000241 DASVLN010000000
VERSION     DASVLN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281201"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..655)
                     /locus_tag="VF453_05540"
     CDS             complement(23..655)
                     /locus_tag="VF453_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7687144.1"
                     /translation="MIRVVIVDDHAIVRSGLRQFLSDHVDLRVTGEAADGREALELVR
                     GGDLDVLLMDLSMPDQSGVDALAAIKARRPDLPVLILSGFPETHYATTLLRQGASGYL
                     NKGCDPDEIVKAIRTVAMGRRYITAQVAEMLADGAAGVNGRPAHEQLSERELQVFLRL
                     AKGETVGQIAEGMFLSVKTVSTYRARTLEKLQLETNSDLTYYALKNGLIQ"
     gene            799..2226
                     /locus_tag="VF453_05545"
     CDS             799..2226
                     /locus_tag="VF453_05545"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX7687145.1"
                     /translation="MSFLRTLETARRSRFAVPVALLLGLAVLLTNELGNRRALAVIES
                     REALLDARVEVSLLLREVIAAESGQRGYLLTGRPEYKEPYIRAAATVDAQLAKIARMY
                     ARWPDREADVKKLDELTRQRMSELNTTITLYESQRSDGWRDLVLTDIGRETMLAIEQQ
                     ARAMARVEGARIDESRELLQETLLFSRIGIAALVFLSLAALLLYARQARRFDAERVER
                     AAALRFERDRLEAEVVRRTREITQIATHLQTAREDERSSLARELHDELGGLLTAAKLD
                     VARMRSRLAGAPPEVTERMGHLVKTLDEGIALKRRIIEDLRPSSLNNLGLKAALEILC
                     AEFAERSEIAVTTEIEDLPFGESARLTVYRLVQEALTNVAKYAAAKRVHVGVHGNGDS
                     AEIEVRDDGVGFDPSAARATAHGLAGMRFRVQSGRGELRIRSKRGQGTTIHARLPLED
                     RVPDTGPAANDLSVLLGPGSFPPAA"
     gene            2396..2707
                     /locus_tag="VF453_05550"
     CDS             2396..2707
                     /locus_tag="VF453_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7687146.1"
                     /translation="MIQRLAIAVFAGALTVLSAGCAVQRHQETVGAYVDDSAVTTKVK
                     AKFAEDPTVSAMAIKVDTMKGVVQLSGFAKSEDERMMAERLARNTNGVRDVRNDIVVK
                     H"
     gene            complement(2929..3117)
                     /locus_tag="VF453_05555"
     CDS             complement(2929..3117)
                     /locus_tag="VF453_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687147.1"
                     /translation="MISAVSTTNKGPVSPPALFDSNWVPQGYVGPVALPGTGRIVYWT
                     GRIAIGLRYAPGGRQTND"
     gene            3270..3611
                     /locus_tag="VF453_05560"
     CDS             3270..3611
                     /locus_tag="VF453_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7687148.1"
                     /translation="MPDPALPSDGSPCQACGACCAAFRVSFHWLEAEGSALPEPLAER
                     VSEHLLCMRGTWSGRPRCVALAGEVGRAVACTVYPHRPSPCREVQAGDAQCTRARAAH
                     GLPPLGTGAAA"
     gene            3761..4363
                     /locus_tag="VF453_05565"
     CDS             3761..4363
                     /locus_tag="VF453_05565"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7687149.1"
                     /translation="MLQFKKSVSSLLAAAALSALSAHASAGVTYYTSQSDYAAALASS
                     TTETFDSTPLASGLSFVSNAGSVGGGLFNDRVVIGSATTTFSFGGDVNGFGGFFDESP
                     GGWGQGLAFTLTLANGSTEVLSQHLDQDGGSFFGFISTDAFSSVKLTGGNESNGVAET
                     YNVDNVQFGSLSTVPENGSMAMLLAGLGMLGVVARRRAGK"
     gene            complement(4466..>6189)
                     /locus_tag="VF453_05570"
     CDS             complement(4466..>6189)
                     /locus_tag="VF453_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687150.1"
                     /translation="PPAPAPAPGPTPPPPAPAPAGAPVIVYTDVPSGPVSGGEGGQGA
                     YLSIFGRNFGDPSALGTTTTVTIGGVAVANYRYLGPSKVAARLGLQELVVQVGHLAGK
                     AAGTALPVVVTAGGGASNADFTFTPNPGRVLFVALDGNDSTAVADDISHPWRTLQNGA
                     GGAYGAMRAGDQVVVRGGTWHDVLGVDGTWLRFGNDASKKGSAPTGAAGTGWIHITAY
                     PGPAGGNAPEDVHYVTPAGGRGGIEGPWSAIRGVSGEYVAISNLHLESSATASSDAAP
                     INLQYNDGPWRIVNNEIGPWPSTLAAPNNAKAGGISGEGVGVEILGNDIHDIACAQGH
                     ATNPLENHAIYIDDDGAYEIAWNVIERIPGGNGLQIFTNGGFSTVTNNVAFHHNIVDG
                     VGKHGINLADGSGANIAVYGNLVANTQYSGLRFNTTDLVGAKVWANTFYATSLHGASD
                     FYGAVSNDWGLPAGAVDFTDNIFFPAPGTNYVGGSVGFFADGKATWSTDLWFGGHDGV
                     PSFASAPRTGDPKFVAAGSDYHLAAGSAAAGTGSAAVSSLVKVDLDGRVRTSPWDIGA
                     YAASGTH"
BASE COUNT          872 a         2192 c         2215 g          910 t
ORIGIN      
        1 cggccgcgcg cgcggcgacg cctcactgga tgaggccgtt cttcagggcg tagtaggtga
       61 ggtcgctgtt ggtctcgagc tggagcttct ccagcgtgcg cgcgcggtag gtgctgaccg
      121 tcttcacgct caggaacatg ccctccgcga tctggcccac cgtctcgccc ttggccaggc
      181 gcaggaagac ctgcagctcg cgctcggaca gctgctcgtg cgccggccgg ccgttcacgc
      241 ccgcggcgcc gtcggccagc atctccgcga cctgcgcggt gatgtagcgc cggcccatgg
      301 ccaccgtgcg gatcgccttg acgatctcgt cggggtcgca tcccttgttc aggtagccgc
      361 tggccccctg gcgcagcagc gtggtggcgt agtgcgtctc cgggaagccg ctgaggatca
      421 gcacgggcag gtcggggcgc cgcgccttga tggcggcgag cgcgtcgacg ccgctctggt
      481 cgggcatcga caggtccatg aggagcacgt cgaggtcgcc gccacgcacg agctcgagcg
      541 cctcgcggcc gtccgccgcc tcgccggtga cgcgcaggtc gacgtggtcg gagaggaact
      601 ggcgaagccc ggagcgaacg atcgcatgat cgtcgacgat cacaacgcga atcatgtagt
      661 ctcccgcggt atcgaagaac tggaaatcag ctgcgcgccg agcggcttga agccctcggc
      721 gcaccaccgg cctgacgtct gggccgcgag tcgaacatcc gtcgggctga gtcgcccaag
      781 tgtagcgggg aacccgacat gtccttcctg agaaccctgg agaccgcccg gcgcagccgt
      841 ttcgcggtgc cggtggcgct ccttctcggc ctggccgtcc tgttgacgaa cgagctcggc
      901 aatcgacgtg ccctcgcggt gatcgaaagc cgcgaggcgc tgctggatgc gcgcgtcgag
      961 gtcagcctgc tgctgcgcga ggtcatcgcc gccgaatccg gccagcgcgg ctacctgctc
     1021 acgggccggc ccgaatacaa ggagccgtac atccgcgccg cggcgaccgt cgacgcgcaa
     1081 ctcgcgaaga tcgcccgcat gtacgcgcgc tggccggatc gagaggccga cgtgaagaag
     1141 ctcgacgagc tgacgcgcca gcgcatgtcg gagctgaaca ccacgatcac gctgtacgag
     1201 agccagcgct ccgacggctg gcgcgacctg gtgctgaccg acatcggccg cgagaccatg
     1261 ctcgccatcg agcagcaggc gcgggcgatg gcgcgcgtcg agggagcgcg catcgacgaa
     1321 tcgcgcgagc tgctgcagga gacgctgctg ttcagccgca tcggcatcgc ggcgctcgtc
     1381 ttcctgagcc tcgcggcgct gctgctgtac gcgcgccagg cgcgccgctt cgacgccgag
     1441 cgcgtggagc gcgccgcggc gctgcgcttc gagcgcgacc gcctcgaggc cgaggtcgtg
     1501 cgccgcacgc gcgagatcac gcagatcgcc acgcacctgc agaccgcgcg cgaggacgaa
     1561 cgcagcagcc tcgcccgcga gctgcacgac gagctgggcg gcctgctgac cgcggcgaag
     1621 ctcgatgtcg cccgcatgcg cagccgcctg gctggcgcac cgcccgaggt caccgagcgc
     1681 atgggccacc tcgtgaagac gctggacgaa ggcatcgcgc tcaagcgccg catcatcgag
     1741 gacctgcggc cgtcgtcgct gaacaacctc gggctgaagg cggcgctcga gatcctgtgc
     1801 gcggaattcg ccgagcgcag cgagatcgcc gtcaccaccg agatcgagga cctgcccttc
     1861 ggcgaatccg cgcgcctgac cgtctaccgg ctggtgcagg aagcgctcac caacgtcgcc
     1921 aagtatgcgg ccgcgaagcg cgtgcatgtc ggcgtgcacg gcaacgggga ctcggcggag
     1981 atcgaggtgc gcgacgacgg cgtcggcttc gacccgagcg ccgcgcgcgc caccgcccac
     2041 gggctggcgg gcatgcgctt tcgggtgcag tcggggcgcg gcgagctgcg gatccgctcc
     2101 aagcgcgggc agggcacgac gatccacgcc cgcctgccgc tggaggaccg cgtgcctgac
     2161 accggcccgg cggccaacga cctcagcgtg ctgctggggc cgggcagctt cccgccggcg
     2221 gcctgagacg gcccgcccct tgcctgaagc ggagctgtcg gccccgtccg acaggtcgag
     2281 gcggcgccgg actatgcctg ccgcgcgccc gcgggcctat cgtttgcagc atcggccgcg
     2341 gcgccaccgt cgcgccgagg gccctacatc aatcacgaca tcgaaggaga acaccatgat
     2401 ccagcgtctc gccatcgccg tcttcgccgg cgccctgacc gtcctgtccg ccggctgcgc
     2461 cgtgcaacgc caccaggaga ccgtcggcgc gtacgtcgac gactccgccg tcaccacgaa
     2521 ggtcaaggcg aagttcgccg aggatccgac cgtgagcgcg atggcgatca aggtcgacac
     2581 gatgaagggc gtcgtgcagc tgtccggctt cgccaagtcc gaggacgagc gcatgatggc
     2641 cgagcgcctc gcgcgcaaca ccaacggcgt gcgcgacgtg cgcaacgaca tcgtcgtcaa
     2701 gcactgatcc cgcgcccgcg gcggcccggt ccgcgggtcg agccgccgcg gctcgtggac
     2761 gaacgacagg cgcccgcggg cgcctgcttt ttttcagtgg gttagatacc cgggcaggca
     2821 tgcgaaggca cgccgccgga tgaagcgggg ccgggaacgc cgcgtgcaat ggaaggcgcg
     2881 gctggaacaa ctgtccgcaa ggtcgcggac ggggaaggcg gcggcgactc agtcgttggt
     2941 ctggcggccg cccggcgcgt agcgcaggcc gatcgcgatg cggccggtcc aatacacgat
     3001 gcggccggtg ccgggcagcg ccacggggcc gacgtagccc tggggcaccc agttcgagtc
     3061 gaacaaggcg ggcggcgaca ccgggccctt gttggtggtc gagacggcgg aaatcatggc
     3121 tcacctccga tgcaactggt aactcttcgg caaacggcgc gcccggccgt ggcgttctcc
     3181 cattggggca gtgcgcaggc gggcggtcta tcccccgtcg gggccatctc cctagctcgg
     3241 cgtggcggcc gagcgacaat gccgccccga tgcccgatcc cgccctgccg tcagacggct
     3301 cgccctgcca ggcctgcggc gcctgctgcg cggcattccg cgtctcgttc cactggctcg
     3361 aggccgaggg gagcgccctg cccgagccgc tggccgagcg cgtgagcgag cacttgctgt
     3421 gcatgcgcgg gacgtggtcg gggcgtccgc gctgcgtggc gctggcgggc gaggtcggcc
     3481 gcgccgtcgc ctgcaccgtc tatccgcacc gccccagccc gtgccgcgag gtgcaggccg
     3541 gcgatgcgca atgcacccgg gcgcgcgccg cccacggcct gccgccgctc ggcacgggcg
     3601 cggcagcctg aggcgctccg gcggctgcca tcgattcctc cccgcttttg gggagacatc
     3661 ttactgtcga gtagctgaat caatccctag aatgcgcacc gggaggggtt gccttttgga
     3721 aaagggctgc tccaagcctg tcattccaca aggaaaatcg atgctgcaat tcaagaagtc
     3781 cgtgtcctcc ctgctggccg ccgctgccct gtcggcactg agcgcccatg catccgccgg
     3841 ggtcacctac tacacctcgc agtccgacta cgccgccgcc ctggcgtcgt ccacgaccga
     3901 gaccttcgac tcgacgccgc ttgcctcggg cctgagcttc gtgtcgaacg ccggctccgt
     3961 cggcggcggc ctgttcaacg atcgcgtcgt catcggcagc gcgacgacga cgttctcgtt
     4021 cggcggcgac gtcaacggct tcggcggctt cttcgacgag tcgcctggcg gctggggcca
     4081 gggcctcgcc ttcacgctga cgctggccaa cggctcgacc gaggtcctgt cgcagcacct
     4141 cgaccaggac ggcggcagct tcttcggctt catctcgacc gacgccttct ccagcgtgaa
     4201 gctgaccggc ggcaacgagt cgaacggcgt cgccgagact tacaacgtcg acaacgtgca
     4261 gttcggctcg ctgtccaccg tcccggagaa cggttcgatg gccatgctgc tggccggcct
     4321 gggcatgctg ggcgtcgtcg cccgccgccg cgccggcaag tgatcgccgc tcgcggcgca
     4381 ggcgcgccgc gacgccgcga cgggcccgct tcggcgggcc tttttcatgg cacggggcgc
     4441 ccgcctcgag accgtggagc ggccctcagt gcgtgccgct cgcggcgtag gcaccgatgt
     4501 cccacgggct ggtgcgcacg cggccgtcga ggtcgacctt cacgagcgag gacaccgcgg
     4561 cgctgcccgt ccccgccgcg gcgctgccgg cggcaaggtg gtagtcgctg cccgccgcga
     4621 cgaacttcgg gtcgcccgtc ctcggcgcgc tcgcgaagga cggcacgcca tcgtgcccgc
     4681 cgaaccagag gtcggtcgac cacgtcgcct tgccgtcggc gaagaagccc accgagccgc
     4741 cgacgtagtt ggtccccggg gcggggaaga agatgttgtc ggtgaagtcg accgcgccgg
     4801 cgggcaggcc ccagtcgttg gacaccgcgc cgtagaagtc cgacgcgccg tgcaggctcg
     4861 tcgcgtagaa cgtgttggcc cacaccttcg cgccgacgag gtcggtggtg ttgaagcgca
     4921 gccccgagta ctgcgtgttc gcgacgaggt tgccgtagac cgcgatgttc gcgccgctgc
     4981 cgtcggccag gttgatgccg tgcttgccga cgccatcgac gatgttgtgg tggaacgcga
     5041 cgttgttggt gaccgtggag aagccgccgt tcgtgaagat ctgcaggccg ttgccgccgg
     5101 ggatgcgctc gatcacgttc caggcgatct cgtaggcgcc gtcgtcgtcg atgtagatcg
     5161 cgtggttctc gagcgggttg gtcgcatggc cctgggcgca ggcgatgtcg tggatgtcgt
     5221 tgccgaggat ctcgacgccc acgccctcgc cgctgatgcc gccggccttc gcgttgttcg
     5281 gcgccgcgag cgtcgacggc cacgggccga tctcgttgtt gacgatgcgc caggggccgt
     5341 cgttgtactg caggttgatc ggcgcggcgt cgctcgacgc ggtcgccgac gattccaggt
     5401 gcaggttgga gatcgcgacg tattcgccgg agacgccgcg gatcgccgac caggggccct
     5461 cgatgccgcc gcggccgccg gccggcgtga cgtagtgcac gtcctcgggc gcgttgccgc
     5521 cggccgggcc cgggtaggcg gtgatgtgga tccagcccgt gccggctgcg ccggtgggcg
     5581 ccgagccctt cttgctcgcg tcgttgccga agcgcagcca ggtgccgtcg acgcccagga
     5641 cgtcgtgcca cgtgccgccg cgcacgacga cctggtcgcc ggcccgcatc gcgccgtacg
     5701 cgccgccggc gccgttctgc agcgtgcgcc acgggtgcga gatgtcgtcg gcgacggcgg
     5761 tcgagtcgtt gccgtccagg gcgacgaaca gcacgcggcc tggattgggc gtgaacgtga
     5821 aatcggcatt gctcgcgccg ccgcccgcgg tcaccacgac gggcagcgcc gtgccggccg
     5881 ccttgcccgc gagatggccg acctgcacga ccagctcctg caacccgagc cgcgcggcca
     5941 ccttggacgg cccgaggtag cggtagttgg cgaccgccac gccgccgatc gtcaccgtgg
     6001 tcgtcgtgcc cagcgcggac gggtcgccga agttgcgccc gaagatggac agataggcgc
     6061 cctgcccgcc ctcgccgccg ctcaccgggc cgctgggcac gtcggtgtag acgatcaccg
     6121 gcgcgccggc cggtgcgggc gccggtgggg gcggcgtcgg cccgggcgcc ggggcgggcg
     6181 ctggcgggg
//
LOCUS       DASVLN010000242        10475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_226323, whole genome shotgun sequence.
ACCESSION   DASVLN010000242 DASVLN010000000
VERSION     DASVLN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10475
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226323"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VF453_05575"
     CDS             complement(<1..524)
                     /locus_tag="VF453_05575"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEX7687151.1"
                     /translation="MLKQLVIVASLIPLAACVTANPDVVSRYDTQRMSSVQDATVLSV
                     RPVVIDGQQSGIGGVSGAVIGGVAGSNVGGPRGSAIVGLVGAIAGGVIGNAVERNATK
                     ENALEIILQMKNGDRRMITQGIGREGFAPGEPVIVVTTGGRARVMKAPPVTTGSAAQS
                     GYAPAAGYEQPASYP"
     gene            complement(754..1311)
                     /locus_tag="VF453_05580"
     CDS             complement(754..1311)
                     /locus_tag="VF453_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX7687152.1"
                     /translation="MDVLQLDVSGRPQAWMTVKEAAVLYACDAVAWTLGEPRHVMRGG
                     VQRATGLQSRIEVHAIIAVRGTIPSRAWRQAPALTNAKLFARDRHVCAYCGAHHPAEE
                     LTREHIVPTSRRGLDSWMNCITACRACNGRKGSRTPEEAGMSLLYLPYVPNLHEDMIL
                     RGRRILADQMEFLLASVPRSSRLHA"
     gene            1673..2386
                     /locus_tag="VF453_05585"
     CDS             1673..2386
                     /locus_tag="VF453_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7687153.1"
                     /translation="MTAVNDAAAALRRPSRPLQKGLQTRATIIEAALALAANKGLEGL
                     SIGSLAEVTGMSKSGVFAHFGSREELQISVIRDYHVKFEEEVFLPSIREPRGLPRLRA
                     MFERWLRRVSYEIDSGCIYISGAVEFDDRPGPVRDALATMVQTWQDALTRAIQMAIDE
                     GHLAPDTEPEQVMFETHGLILALHHDARFLRNPRSVERARRGFERVLAHSMASAPNPV
                     PGPAPGAAEPPKEARPARR"
     gene            2443..4233
                     /locus_tag="VF453_05590"
     CDS             2443..4233
                     /locus_tag="VF453_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7687154.1"
                     /translation="MPQYNPPLRDMQFVLHEVLNIVDELKVMPRHAETDADTINAVLE
                     EGGKFASEVIFPLNLSGDAEGCKLDKTTHEVTPPKGFKEAYKTYVEGGWPSLSCDPEY
                     GGQGLPVALNQCFYEMLNSGNQAWTMYPGLSHGAYEALHAHGTPEQKAMFLPKLTSGE
                     WTGTMCLTEPHCGTDLGLLRTKAEPQADGTYKITGAKIFISAGEHDLSENILHLVLAR
                     LPDAPAGSKGISLFLVPKIKVGKDGSLGERNPIFCAGLEHKMGIHGNATCQMVLEGAV
                     GTMVGEPNKGLAAMFVMMNAARLGVGVQSLGLTEVAYQNAVAYAKDRLQMRALSGPKA
                     PEKAADPIIVHPDVRKMLLTARAYAEGGRALAIHTALLIDKELNHPDEDVRKEAADEV
                     ALLTPIVKAFITDNGWIATSHCMQVYGGHGFIHEWGMEQYVRDARINMIYEGTNTIQS
                     LDLLGRKVLADNGAKLKRFGKKVAQFVEDEGISEAMQEFVNPLADLGDKVTKLTTEIG
                     MKAFQNPDEVGAAAVDYLRVCGHLVFAYFFARMAKVALDKQSSGDPFYKAKLTTARFY
                     FAKLLPETAGLIRSCRAGLKPLMEMDEALF"
     gene            4379..4822
                     /locus_tag="VF453_05595"
     CDS             4379..4822
                     /locus_tag="VF453_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX7687155.1"
                     /translation="MKKAAVAFLLATASVLALAQNATPAGLWKTIDDDGKTEKSLVRI
                     TNDNGVYTGKVEKIFDPAKADAKCDKCDDDRKDKPIVGMTIINGVKQDAEENNLFDGG
                     QILDPANGKTYRVRLKPTEGGKKLEVRGYIGTPMLGRTQTWIRAE"
     gene            4901..7291
                     /locus_tag="VF453_05600"
     CDS             4901..7291
                     /locus_tag="VF453_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX7687156.1"
                     /translation="MSRFQVRKVAVLGAGVMGAQIAAHLVNVKVPVVLFDLPAKSGPR
                     NGIVTKAVDGLKKLKPAPLGVVEDAGLIQQANYEEHLEQLRDCDLVIEAIAERMDWKL
                     DLYTKVAPFIAPHAIVASNTSGLSITKLSEVLPEEIKPRFCGIHFFNPPRYMRLVELI
                     PTPTTRPEILDQLETFVTSALGKGVVRAKDTPNFVANRVGIAGMLATIIEAEKFGLTY
                     DVVDDLTGKKLGRASSGTFRTADVVGLDTMAHVIKTLQDNLKDDPFFPSYATPPVVGR
                     LIEQGALGQKAGAGFFKKVGKDILRLDPSKGEYVPAGAKADPIVDRILKKPAAERLKL
                     LRESTNPQAQFVWAILRDGFHYAAVHLADIADSAREVDFAMRWGFGTKQGPFELWQDA
                     GWKQVAEWIKEDIDAGKALSSAPLPAWVFDGPVPEKGVHTPDGSWSAAQGKYLPASQL
                     DVYKRQPFPETVLGAGAANPLKSGTEEFKNEEVRVWTLDGEVLIASITAKLHLISPTV
                     TEGLLKAVEIAESKYKGLVIWSPDEAFSAGANLEALMPVFMKSGAKGIAPEEKKLQDM
                     MLRVRYAAVPVVAAVRGIALGGGCELAVHCAARVAAMESYMGLVEVGVGLIPGGGGLT
                     YIARRAAEAAAAVPGSDLLSFLKEGFTAAATAKVGTSALESRKIGYLLESDIVVPHID
                     ELLHVASTHAKALFDAGYRAPLKRPIPVAGRSGIATIKASLVAMRDGGFISQHDFHIG
                     SLIADVCCGGEVDAGTPVTEEYLMALERKHFCSLLENPKTQERIMGMLQTGKPVRN"
     gene            7306..8505
                     /locus_tag="VF453_05605"
     CDS             7306..8505
                     /locus_tag="VF453_05605"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7687157.1"
                     /translation="MSKQISDAYIVAATRTPIGKSGRGYFKNTRPDDLLVAALQGALK
                     QVPSLDPKAIEDAIVGCAMPEGEQGLNFARTGVLLAGLPNTVGGITVNRFCASGLSAV
                     QMAADRIRVGEADVMIAAGAESMSMVPMSGNKPSFNPHVFDKDENIGIAYGMGLTAEK
                     VASQWKVSREAQDEFALGSHQKALKAIAAGEFKDEITPVEVFTRTPNLLTGEIDVTSR
                     WVDVDEGPRPDTSMEGLAKLKAVFANKGSVTAGNSSQTSDGAGALIVVSERALKQFNL
                     TPLARFVSFASKGVPPEIMGIGPIEAIPAALKSAGLNLSQLDWIELNEAFAAQALAVI
                     NTVGLDATKVNPMGGAIALGHPLGATGAIRSATVVHALRRHNLKYGMVTMCVGTGQGA
                     AGIFERL"
     gene            8631..9521
                     /locus_tag="VF453_05610"
     CDS             8631..9521
                     /locus_tag="VF453_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7687158.1"
                     /translation="MTRARAFRELTLHAADGFALGGRRYGDAARARAGIVIAPAMGVP
                     QSYYADFAAWLAAQGYAVLSFDYRGIGASRPAGSLREVRADVVTWAERDADAAIAQLE
                     SELRADTPAGIAPRPLHWLGHSLGGQILGLVPRTERLARVVTVGSGSGYWRENAAGAR
                     RWSWWLWFVAVPLALRVAGYFPGRRLRKIGDLPAGVMAQWRRWCLSPDYMMGEGGPAL
                     RARYASVHNPILSLSFTDDEYMSARNIDSMHGFYAGAPREMRRLAPRDVGASAIGHFG
                     FFRARFEATLWPQVARFLAA"
     gene            9560..10321
                     /locus_tag="VF453_05615"
     CDS             9560..10321
                     /locus_tag="VF453_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7687159.1"
                     /translation="MTIRTATTEGVATIEIARPEKKNALTQAMYTAMAEALAAADADA
                     SVRAILITGQPGVFTSGNDLEDFMQRPPAGEESPVFRFMKALLDTGKPVVAAVTGAAI
                     GIGTTMLLHCDLVYVSDEARLAMPFVGLGLVPEYASSLLVPQLVGHAKATELLLLGEP
                     FTGERAVEMGIANAVLPAAEVVNHARRMAERFKALPPGAVRESKALMRRGQQALVRET
                     VAVEGEAFGKRLRSPEAMEAFQAFFQKRKPDFSKF"
BASE COUNT         1687 a         3747 c         3565 g         1476 t
ORIGIN      
        1 ggatagctcg cgggctgctc gtagccggca gccggcgcat agccgctctg cgcggcgctg
       61 ccggtggtca ccggcggcgc cttcatgacg cgcgcgcggc cgccggtcgt caccacgatc
      121 accggctcgc cgggcgcgaa gccctcccgg ccgatgccct gggtgatcat gcggcggtcc
      181 ccgttcttca tctgcaggat gatctcgagc gcgttctcct tggtggcgtt gcgctcgacc
      241 gcgttgccga tcacgccgcc ggcgatcgcg ccgacgagtc cgacgatggc agagccgcgc
      301 ggcccgccga cgttggagcc ggcgacaccg ccgatcaccg cgccgctgac cccgccgatg
      361 ccgctctgct ggccgtcgat cacgaccggg cgcaccgaca gcacggtggc gtcctgcacg
      421 gaggacatgc gctgggtgtc gtagcggctg acgacgtcgg ggttggcggt cacgcacgcg
      481 gccagcggga tgagcgaggc gacgatgacg agctgcttga gcatggaagt tctccgggag
      541 tcctgtccgc ggggcgggcg atccggcccg cgtcgacggt gacacatatg gttgcgctgg
      601 gctgcgaaag caattgcggg cgccgcgcgt cccaaagtaa agaaatgtgc gcccttgtcg
      661 ggctgggagt gcgcgcaagg ggcattcgtt ccccggcatt ctgcgtcgcg ggcgggcagg
      721 gcgcgcgccc gcggcccgcg gttcagcctg ccctcaggcg tgcaggcggc tcgagcgcgg
      781 cacgctcgcg agcaggaact ccatctggtc ggccaggatg cggcggccgc gcaggatcat
      841 gtcctcgtgc aggttgggca cgtagggcag gtagagcagg ctcatgccgg cctcctcggg
      901 cgtgcggctg cccttgcggc cgttgcaggc gcggcaggcg gtgatgcagt tcatccatga
      961 gtccagcccg cgacgcgacg tcggcacgat gtgctcgcgc gtgagctcct cggccggatg
     1021 gtgcgcgccg cagtacgcgc agacgtggcg gtcgcgcgcg aacagcttgg cgttggtgag
     1081 ggcgggcgcc tggcgccagg cgcgcgaggg gatcgtgccg cgcacggcga tgatggcgtg
     1141 cacctcgatg cgcgactgca gccccgtggc gcgctggacg ccccctcgca tcacgtggcg
     1201 cggctcgccg agcgtccagg ccacggcgtc gcaggcgtac aggaccgcgg cctccttgac
     1261 cgtcatccag gcctgcggcc ttcccgacac gtccagttgc agcacatcca tcaccgtcac
     1321 ggcggacaca ctccttgtgc cttcaagcat accccgccac tggatggatc cacaacccgc
     1381 gcgggcggga cgcgtgccgc gggccccggc gcgccccgcc ccgggggatc gccgcggcgg
     1441 cctcttgcgc gtttaccccg atggaaaatg aacgatcgtt cttttccgtg tcccgagtgt
     1501 gacttccgag gcgtttttct aggatccggg acctaaaaaa agtggtcgac accattggcg
     1561 cggcgacccg aagtctgcaa aatagaacga ccgttcgatt tattccgggc ccgccgatgc
     1621 ggcgccggcg cgatccggac ggactcgcca ccgatcccgg ctcgccgccg ggctgaccgc
     1681 cgtgaacgac gccgccgccg ctcttcgccg accgtcccgt cccctgcaga agggcctgca
     1741 gacgcgcgcc accatcatcg aggcagcgct ggcgctggcc gcgaacaagg gcctcgaggg
     1801 cctgtcgatc ggctcgctgg ccgaagtcac gggcatgagc aagtccggcg tcttcgcgca
     1861 cttcggctcg cgcgaggagc tgcaaatttc cgtcatccgg gactaccacg tcaagttcga
     1921 ggaggaagtc ttcctcccgt cgatccgcga gccgcgcggc ctgccgcgcc tgcgcgccat
     1981 gttcgagcgg tggctgcggc gcgtgtccta cgagatcgac tcgggctgca tctacatctc
     2041 cggcgccgtc gagttcgacg accgtcccgg ccccgtccgc gacgcgctcg cgacgatggt
     2101 gcagacctgg caggacgcgc tgacgcgcgc gatccagatg gccatcgacg agggccacct
     2161 cgcgcccgac accgagccgg agcaggtcat gttcgagacc cacggcctca tcctcgcgct
     2221 gcaccacgac gcgcgcttcc tgcgcaaccc gcgcagcgtc gagcgcgccc gccgcggctt
     2281 cgagcgcgtg ctcgcgcact cgatggcgtc cgccccgaat cccgtgcccg ggcccgcgcc
     2341 cggcgccgcc gagccgccga aggaggcgcg ccccgcgcgc cgctgaacgg ccccgtccgg
     2401 ttcccaccgc cccttccttc ccgagctcca ggagctttcg acatgcccca atacaacccg
     2461 ccgctccgtg acatgcagtt cgtgctgcac gaggtcctga acatcgtcga cgagctgaag
     2521 gtcatgcccc ggcacgcgga gaccgacgcc gacaccatca acgcggtgct cgaggaaggc
     2581 ggcaagttcg cgtccgaggt gatcttcccg ctgaacctga gcggcgacgc cgagggctgc
     2641 aagctcgaca agacgacgca cgaggtgacg ccgcccaagg gcttcaagga ggcgtacaag
     2701 acctacgtcg aaggcggctg gccgtcgctg tcgtgcgacc cggagtacgg cggccagggc
     2761 ctgcccgtcg cgctgaacca gtgcttctac gagatgctca acagcggcaa ccaggcctgg
     2821 acgatgtatc ccggcctgtc gcacggcgcg tacgaggcgc tgcacgcgca tggcacgccg
     2881 gagcagaagg cgatgttcct gcccaagctg acctccggcg aatggacggg cacgatgtgc
     2941 ctgaccgagc cgcactgcgg caccgacctc ggcctgctgc gcacgaaggc cgagccccag
     3001 gccgacggca cctacaagat caccggcgcc aagatcttca tctccgccgg cgagcacgac
     3061 ctgtccgaga acatcctgca cctggtgctc gcgcgcctgc cggacgcgcc ggccggctcc
     3121 aagggcatct cgctgttcct cgtgcccaag atcaaggtcg gcaaggacgg ctcgctgggc
     3181 gagcgcaacc cgatcttctg cgcgggcctg gagcacaaga tgggcatcca cggcaacgcc
     3241 acttgccaga tggtgctgga aggcgcggtc ggcacgatgg tcggcgagcc caacaagggc
     3301 ctggccgcga tgttcgtgat gatgaacgcg gcccgcctgg gcgtgggcgt gcagtcgctg
     3361 ggcctgaccg aggtcgcgta ccagaacgcg gtcgcctacg cgaaggatcg cctgcagatg
     3421 cgcgcgctgt ccggtccgaa ggcgcccgag aaggccgccg acccgatcat cgtccacccc
     3481 gacgtgcgca agatgctgct gaccgcgcgc gcctacgccg agggcggccg cgcgctggcc
     3541 atccacaccg cgttgctgat cgacaaggaa ctcaaccacc ccgacgagga cgtgcgcaag
     3601 gaggccgccg acgaggtcgc gctgctgacg cccatcgtca aggccttcat caccgacaac
     3661 ggctggatcg ccacctcgca ctgcatgcag gtctacggcg gccacggctt catccacgag
     3721 tggggcatgg agcagtacgt gcgcgacgcg cggatcaaca tgatctacga gggcacgaac
     3781 accatccagt cgctggacct gctgggccgc aaggtgctgg ccgacaacgg cgccaagctc
     3841 aagcgcttcg gcaagaaggt cgcgcagttc gtcgaggacg agggcatcag cgaggcgatg
     3901 caggaattcg tgaacccgct ggccgacctg ggcgacaagg tcaccaagct gaccaccgag
     3961 atcggcatga aggcgttcca gaacccggac gaggtcggtg ccgcggcggt cgactacctg
     4021 cgcgtatgcg gccacctcgt gttcgcctac ttcttcgccc gcatggccaa ggtcgcgctc
     4081 gacaagcagt cgtcgggcga cccgttctac aaggccaagc tgaccaccgc gcgcttctac
     4141 ttcgccaagc tgctgcccga aaccgccggc ctcatccgca gctgccgcgc cggcctgaag
     4201 ccgctgatgg agatggacga agccctcttc tgatcggacg cgcccggcgc gccgcgcgcg
     4261 cggcttgccg ggccatcccc gatccgaggc gtcgccggca ggacagcgat tcgcggcgaa
     4321 tcggagcgat catcggcccg cggcccggcc gcccacacca acccaggaga cttcctcgat
     4381 gaaaaaagct gctgttgcct tcctcctcgc caccgccagc gtgctggccc tcgcccagaa
     4441 cgcgacgccc gcgggcctgt ggaagaccat cgacgacgac ggcaagaccg agaagtcgct
     4501 cgtgcgcatc accaacgaca acggcgtcta cacgggcaag gtcgagaaga tcttcgatcc
     4561 ggccaaggcc gacgcgaagt gcgacaagtg cgacgacgac cgcaaggaca agcccatcgt
     4621 cggcatgacc atcatcaatg gcgtgaagca ggacgccgag gagaacaacc tcttcgacgg
     4681 cggccagatc ctcgacccgg ccaacggcaa gacctaccgc gtccgcctga agcccaccga
     4741 aggcggcaag aagctcgaag tgcgcggcta catcggcacg ccgatgctgg gccgcacgca
     4801 gacctggatc cgcgccgagt aagcgcgaca gcaaagcaag cggggcgcgg cggcggtgct
     4861 gcccgtcgcc cgccacgttc cacccaccgg agatacacaa gtgagtcgat tccaagttcg
     4921 caaggtcgcc gtcctcggcg ccggcgtgat gggcgcgcag atcgcggccc atctggtcaa
     4981 cgtgaaggtg ccggtcgtcc tgttcgacct gcccgcgaag tcggggccca ggaacgggat
     5041 cgtcaccaag gccgtcgacg gcctgaagaa gctgaagccg gcgcccctgg gcgtggtcga
     5101 ggacgcgggt ctgatccagc aggccaacta cgaggagcac ctcgagcagc tgcgcgattg
     5161 cgacctcgtc atcgaagcca ttgccgaacg catggactgg aagctggacc tctacacgaa
     5221 ggtcgcgccg ttcatcgccc cgcacgccat cgtcgcgtcc aacacctcgg gcctgtcgat
     5281 caccaagctg tcggaagtcc tgcccgagga gatcaagccg cgcttctgcg gcatccactt
     5341 cttcaacccg ccgcgctaca tgcgcctggt ggagctgatc ccgacgccga ccacgcgccc
     5401 cgagatcctc gaccagctcg agaccttcgt cacctccgca ctcggcaagg gcgtggtgcg
     5461 cgcgaaggac acgcccaact tcgtcgccaa ccgcgtcggc atcgccggca tgctggccac
     5521 gatcatcgag gcagagaagt tcggcctcac gtacgacgtc gtcgacgacc tgacgggcaa
     5581 gaagctcggc cgcgcgagct cgggcacctt ccgcaccgcg gacgtcgtcg gcctcgacac
     5641 gatggcccac gtcatcaaga cgctgcagga caacctgaag gacgatccgt tcttcccgag
     5701 ctacgccacg ccgccggtcg tcggccgcct gatcgagcag ggcgcgctgg gccagaaggc
     5761 gggcgccggc ttcttcaaga aggtcggcaa ggacatcctg cgcctggatc cgtccaaggg
     5821 cgagtacgtg ccggccggcg cgaaggcgga cccgatcgtc gaccgcatcc tcaagaagcc
     5881 cgcggccgag cgcctgaagc tgctgcgcga gtccaccaac ccgcaggcgc agttcgtgtg
     5941 ggccatcctg cgcgacggct tccactacgc cgccgtgcac ctggccgaca tcgccgactc
     6001 ggcgcgcgag gtcgacttcg cgatgcgctg gggcttcggc accaagcagg gcccgttcga
     6061 gctgtggcag gacgccggct ggaagcaggt ggccgagtgg atcaaggaag acatcgacgc
     6121 cggcaaggcg ctgtcgtccg caccgctgcc cgcctgggtg ttcgatggcc cggtcccgga
     6181 aaagggcgtg catacccccg atggttcctg gagcgccgcg cagggcaagt acctgccggc
     6241 gtcgcagctc gacgtgtaca agcgccagcc cttccccgag acggtgctcg gcgccggcgc
     6301 ggccaatccg ctgaagtcgg gcaccgagga gttcaagaac gaggaagtcc gcgtctggac
     6361 gctggacggc gaagtgctga tcgccagcat caccgccaag ctgcacctga tctcgccgac
     6421 cgtgaccgaa ggcctgctga aggccgtcga gatcgccgag agcaagtaca agggcctggt
     6481 gatctggtcg ccggacgagg ccttctccgc cggcgcgaac ctcgaggcgc tgatgccggt
     6541 cttcatgaag tccggcgcca agggcatcgc accggaagag aagaagctgc aggacatgat
     6601 gctgcgcgtg cgctacgccg cggtgccggt cgtcgcggcg gtgcgcggca tcgcgctggg
     6661 cggcggctgc gagctggcgg tgcactgcgc cgcgcgcgtg gccgcgatgg agagctacat
     6721 gggcctcgtc gaggtcggcg tcggcctgat tccgggcggc ggcggcctga cctacatcgc
     6781 gcgccgggcc gccgaggccg cggccgcggt gccgggttcc gacctgctgt cgttcctgaa
     6841 ggaaggcttc accgccgccg cgacggccaa ggtcggcacg agcgcgctcg agtcccgcaa
     6901 gatcggctac ctgctcgagt ccgacatcgt cgtgccgcac atcgacgagc tgctgcacgt
     6961 cgcctcgacg cacgccaagg cgctgttcga cgccggctac cgcgcaccgc tcaagcgtcc
     7021 gatcccggtc gccggccgct ccggcatcgc caccatcaag gcctcgctgg tcgcgatgcg
     7081 cgacggcggc ttcatcagcc agcacgactt ccacatcggc agcctcatcg ccgacgtgtg
     7141 ctgcggcggc gaggtcgacg ccggcacgcc ggtgaccgag gaatacctga tggcgctgga
     7201 gcgcaagcac ttctgctcgc tgctcgagaa ccccaagacg caagaacgca tcatgggcat
     7261 gctccagacc ggcaagccgg tccgcaactg attggacgaa cgaacatgag caagcaaatt
     7321 tccgacgcct acatcgtcgc ggccacccgc acgccgatcg gcaagtccgg ccggggctac
     7381 ttcaagaaca cgcgcccgga cgacctgctg gtcgcggcgc tgcagggcgc gctcaagcag
     7441 gtgcccagcc tcgacccgaa ggcgatcgag gacgcgatcg tcggctgcgc gatgcccgag
     7501 ggcgagcagg gcctgaactt cgcccgcacc ggcgtgctgc tggccggcct gcccaatacg
     7561 gtcgggggca tcacggtgaa ccgcttctgc gcgtccggcc tgtcggccgt gcagatggcc
     7621 gcggaccgca tccgcgtcgg cgaggccgac gtgatgatcg ccgccggcgc ggagagcatg
     7681 agcatggtgc cgatgagcgg caacaagccc tcgttcaacc cgcatgtctt cgacaaggac
     7741 gagaacatcg gcatcgccta cggcatgggc ctgacggcgg agaaggtcgc cagccagtgg
     7801 aaggtctcgc gcgaggcgca ggacgagttc gcgctcggct cgcaccagaa ggcgctcaag
     7861 gccatcgccg ccggcgagtt caaggacgag atcacgccgg tcgaggtctt cacgcgcacg
     7921 ccgaacctgc tgacgggcga gatcgacgtg acgagccgct gggtcgacgt cgacgagggc
     7981 ccgcgtcccg acacgtcgat ggaagggctc gccaagctca aggccgtgtt cgcgaacaag
     8041 ggttcggtga ccgccggcaa cagctcgcag acctcggacg gcgccggcgc gctgatcgtc
     8101 gtcagcgagc gcgcgctcaa gcagttcaac ctgacgccgc tcgcgcgctt cgtgagcttc
     8161 gcgtccaagg gcgtgccgcc cgagatcatg ggcatcggcc cgatcgaggc cattcccgcg
     8221 gcgctgaagt cggccggcct gaacctgtcg cagctggact ggatcgagct gaacgaggcc
     8281 ttcgccgccc aggcgctggc cgtgatcaac acggtcggcc tcgacgccac gaaggtgaat
     8341 ccgatgggcg gcgcgatcgc cctcggccac ccgctgggcg cgaccggcgc gatccgttcc
     8401 gcgaccgtcg tgcacgcgct gcgccgccac aacctgaagt acggcatggt gacgatgtgc
     8461 gtcggcaccg gccagggcgc cgccggcatc ttcgagcgcc tgtaatccca cgcccggccc
     8521 gcggccgtcg gctgcacgag taccccacga aggtactcgt cgctcccgtc gccgcgggcc
     8581 ttttcgcatc cgtcgacgcg tatcccagac cttccccacg aggcgaagcc atgacccgtg
     8641 cccgcgcgtt ccgagagctg accctgcacg ccgcggacgg cttcgcgctc ggtggccgcc
     8701 gctacggcga cgccgcccgt gcgcgcgcgg gcatcgtcat cgcgccggcg atgggcgtgc
     8761 cgcagtcgta ctacgccgac ttcgcggcct ggctcgcggc gcagggctac gcggtgctga
     8821 gcttcgacta ccgcggcatc ggcgcgtcgc ggcccgcggg ctcgctgcgc gaggtgcgcg
     8881 ccgacgtggt cacatgggcc gagcgcgacg ccgacgccgc gatcgcgcag ctggagagcg
     8941 agctgcgcgc cgatacgccc gccggtatcg cgccgaggcc gctgcactgg ctcggccaca
     9001 gcctgggcgg ccagatcctc ggcctcgtgc cgcgcacgga gcgcctcgcg cgcgtcgtga
     9061 cggtgggctc gggcagcggc tactggcgcg agaacgcggc cggcgcgcgg cgctggtcgt
     9121 ggtggctgtg gttcgtcgcc gtgccgctcg cgctgcgcgt ggccggctac ttccccggcc
     9181 ggcggctgcg caagatcggc gacctgcccg ccggcgtgat ggcgcagtgg cgccgctggt
     9241 gcctgagccc cgactacatg atgggcgagg gcggcccggc gctgcgggca cgctacgcga
     9301 gcgtccacaa tccgatcctg agcctgagct tcaccgacga cgagtacatg tccgcgcgca
     9361 acatcgactc gatgcacggc ttctacgccg gcgcaccgcg cgagatgcgc cgcctggccc
     9421 cgcgcgacgt cggcgcatcc gcgatcggcc acttcggctt cttccgcgcg cgcttcgagg
     9481 cgacgctgtg gccccaggtc gcacgcttcc tcgcggcgtg aatcgtccgt ccccaacacc
     9541 gcatccacga gagacctcca tgaccatccg cactgccacg accgagggcg tcgccacgat
     9601 cgagatcgcg cgccccgaga agaagaacgc cctcacgcag gcgatgtaca cggcgatggc
     9661 cgaggccctg gcggcggccg atgccgacgc ctccgtgcgc gcgatcctga tcaccgggca
     9721 gccgggcgtc ttcacctcgg gcaacgacct cgaggacttc atgcagcgcc cgcccgcggg
     9781 cgaggaatcg ccggttttcc gtttcatgaa ggcgcttctg gacacgggca agcccgtcgt
     9841 cgcggccgtc accggcgcgg cgatcggcat cggcacgacg atgctgctgc actgcgacct
     9901 cgtctacgtg agcgacgagg cgcgcctggc gatgcccttc gtcggcctcg gcctggtgcc
     9961 ggagtacgcg tccagcctgc tcgtcccgca gctggtcggc cacgcgaagg cgacggagct
    10021 gctgctgctg ggcgagccct tcaccggcga gcgcgcggtg gagatgggca tcgccaacgc
    10081 ggtgctgccg gcggccgagg tcgtcaacca cgcgcgccgc atggccgagc gcttcaaggc
    10141 gctgccgccg ggcgcggtgc gcgagagcaa ggcgctgatg cgtcgcggcc agcaggcgct
    10201 ggtgcgcgag accgtcgcgg tcgagggcga ggccttcggc aagcggctgc gcagccccga
    10261 ggcgatggag gcgttccagg ccttcttcca gaagcgcaag ccggacttct ccaagttctg
    10321 acgtggcggc gaggcccctc ccgtacgtcg acgacgagcg cccgcgcgcg ccgcgtgccg
    10381 cgcgcgcgtc cctgcgcctg cgcggcacgc cgccgtccgc tgccttgacg ctcgcgacca
    10441 agctcgcgct gttgccgctg ctgctgctgc agggc
//
LOCUS       DASVLN010000243         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_442603, whole genome shotgun sequence.
ACCESSION   DASVLN010000243 DASVLN010000000
VERSION     DASVLN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442603"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VF453_05620"
     CDS             complement(<1..521)
                     /locus_tag="VF453_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687160.1"
                     /translation="MNTALRIDAPWRLVGPWYRWTHPGLPADGRASRPAIQMFAGDDF
                     VQAFLQRPQHSLRYDPAIDVVSHYDLVSAVPGGALAAKVSTLFAVNAKGDPAGPGDAK
                     FRARLAPSALRKLFQPTHDRHYLVTCELHCDLPGFPSAGRAGVCQAGFVVRRRRSLVP
                     AGITAGDIDAQAAP"
     gene            complement(518..1177)
                     /locus_tag="VF453_05625"
     CDS             complement(518..1177)
                     /locus_tag="VF453_05625"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX7687161.1"
                     /translation="MIAGPHDGDGARWYGLYPAKVVDLVDPDGKGRIKVQFPSFGEDG
                     ATVVAWATLLSPYADRDQGLEILPEPQSQVVVGFEAGEPARPYIVGACWNGRRAPPER
                     ATAANDLRTLRTRSGSELQFDDSAGAAKVTLSMKSGHRLVLDDEAQQVTFQHANGCSI
                     EINVAGQVTITANATVEVNASGVNVHAPSVVCDGLLQCQTLIATSVVSASYTPGAGNV
                     W"
     gene            complement(1174..2268)
                     /locus_tag="VF453_05630"
     CDS             complement(1174..2268)
                     /locus_tag="VF453_05630"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HEX7687162.1"
                     /translation="MPLPFLPLQASTIPEIKVDGQANADLARDLVRLEVEEDVYGMKR
                     LAATLMGTGPRDGARDEPLNWMDGRVLDFGTELRVSMGPTTAREEVFAGKLSALELSV
                     DQGRTPEVVCLAEDALMDLRMTRRFRTYEQASESELVRQIAAKHGLTAQCDVEGPTWA
                     MVQQWNQSDLAFLRERARRLAAEVWVDGSRLRMATRDKRGGASVTLIQGNNLVQARLR
                     ADLAHQRSRLTVGGFDDGADDRLDESADTSAIDAEAQGHRHGAAVLRQALGERASYHV
                     RSVPLKGDEARALAKAALLWRARRFVTVSGIAEGSTALRVGAQLRLERVSPVFEGDGY
                     YVTRIRHQFDLADGYRTHFDAEREWIGSGS"
     gene            complement(2291..2476)
                     /locus_tag="VF453_05635"
     CDS             complement(2291..2476)
                     /locus_tag="VF453_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687163.1"
                     /translation="MPVTIHELESEIDVRPADGDGTGQEVGASTQPTPAAQESWQESA
                     RREHQRLARTAAWGHDD"
BASE COUNT          305 a          922 c          960 g          378 t
ORIGIN      
        1 ggcgcggcct gcgcgtcgat gtcgcccgcg gtgatgccgg ccgggacgag gctgcgccgg
       61 cgccgcacga cgaagccggc ctggcacacg ccggcgcgcc cggcgctggg gaagccgggc
      121 aggtcgcagt gcagctcgca ggtgacgaga tagtgccgat cgtgcgtcgg ctggaacagc
      181 ttgcgcagcg cgctgggcgc cagtcgggcg cggaacttcg catcgccggg tccggccgga
      241 tcgcccttgg cattgacggc gaagagcgtc gagaccttgg ccgcgagcgc gccgcccggc
      301 accgcgctga cgaggtcgta gtggctgacg acgtcgatgg cggggtcgta ccggagcgag
      361 tgctgcggcc gctgcaggaa ggcctgcacg aagtcgtcgc ccgcgaacat ctggatcgcg
      421 ggccggctgg cgcgcccgtc cgcaggcagc cccggatgcg tccagcggta ccacgggccg
      481 acgagccgcc agggcgcgtc gatacggagt gcggtattca ccatacgttg cccgcgcccg
      541 gcgtgtagct ggcggagacg accgaggtcg cgatcagcgt ctggcactgc agcaggccgt
      601 cgcagacgac gctgggggcg tgcacgttca cgccggatgc gttgacctcg accgtcgcgt
      661 tcgccgtgat cgtgacctgg ccggccacgt tgatctcgat gctgcagccg ttggcgtgct
      721 ggaacgtgac ctgctgcgcc tcgtcgtcga gcacgagccg gtggccgctc ttcatcgaca
      781 gcgtcacctt ggccgcgccg gcgctgtcgt cgaactgcag ctcgctgccc gagcgcgtcc
      841 gcagcgtgcg aaggtcgttg gccgcggtgg cgcgctccgg cggcgcgcgg cggccgttcc
      901 agcaggcgcc gacgatgtag gggcgcgcgg gctcgccggc ctcgaagccg acgaccacct
      961 gcgactgcgg ctcgggcagg atctccaggc cctggtccct gtccgcgtag ggcgacagca
     1021 gcgtcgccca cgcgacgacg gtcgcgccat cctcgccgaa ggaggggaac tgcaccttga
     1081 tgcggccctt gccgtccggg tcgacgaggt cgaccacctt ggccgggtac aggccgtacc
     1141 agcgggcgcc gtcgccgtcg tgcgggcccg cgatcacgat ccgctcccga tccattcgcg
     1201 ctccgcgtcg aagtgcgtgc ggtagccgtc ggcgaggtcg aattggtgcc gaatgcgcgt
     1261 gacgtagtag ccgtcgccct cgaacaccgg gctcacgcgc tcgaggcgca gctgcgcgcc
     1321 gacgcgcagg gcggtcgatc cctcggcgat gccgctgacc gtgacgaatc gccgcgcgcg
     1381 ccagagcagc gcggccttcg cgagcgcgcg cgcctcgtcg cccttgagcg gcacgctgcg
     1441 cacgtggtag ctcgcgcgct cgccgagcgc ctgccgcagc acggcggcgc cgtggcggtg
     1501 cccctgggcc tcggcgtcga tggcgctcgt gtcggcgctc tcgtcgagcc tgtcgtccgc
     1561 gccgtcgtcg aagccgccga cggtcagccg cgagcgctgg tgcgccaggt cggcgcgcag
     1621 ccgcgcctgc acgaggttgt tgccctggat cagcgtgacg ctcgcgccgc cgcgcttgtc
     1681 gcgcgtggcc atgcgcagcc ggctgccgtc gacccagacc tcggcggcga ggcggcgtgc
     1741 gcgctcgcgc aggaaggcga ggtcgctctg gttccactgc tgcaccatcg cccaggtcgg
     1801 gccctcgacg tcgcattgcg cggtcaggcc gtgcttggcg gcgatctggc gcacgagctc
     1861 cgactcgctg gcctgctcgt aggtgcggaa gcgtcgcgtc atgcgcaggt ccatcaacgc
     1921 gtcctcggcc aggcagacga cctcgggcgt gcggccctgg tcgaccgaga gctccagcgc
     1981 gctcagcttg ccggcaaaga cctcctcgcg cgcggtcgtc gggcccatcg agacgcgcag
     2041 ctccgtgccg aagtccagca cgcggccgtc catccagttc agcggctcgt cgcgcgcgcc
     2101 atcgcgcggg ccggtgccca tcaacgtcgc ggccagccgc ttcatgccgt acacgtcctc
     2161 ctcgacctcg aggcgcacca ggtcgcgcgc caggtcggcg ttggcctggc cgtcgacctt
     2221 gatctcgggg atcgtcgacg cttgcagcgg aaggaatggc agcggcatgg cgcgaggact
     2281 cctcggtcgc tcagtcgtcg tgtccccagg ccgccgtgcg ggcgagccgc tggtgctcgc
     2341 gccgcgcgct ttcctgccag gactcctgtg ccgccggcgt gggctgcgtg ctcgcgccga
     2401 cctcctggcc ggtgccgtcc ccgtcggcgg ggcgcacgtc gatctcggac tcgagctcgt
     2461 ggatggtgac gggcatggcg gtacctctgg cggtatgggt cgaggtcagc gcgcgcggcg
     2521 gcggccgcgg cagccgcagc cggggcggcg cgcgtccggt gcgct
//
LOCUS       DASVLN010000244         3336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_442704, whole genome shotgun sequence.
ACCESSION   DASVLN010000244 DASVLN010000000
VERSION     DASVLN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442704"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VF453_05640"
     CDS             <1..714
                     /locus_tag="VF453_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687164.1"
                     /translation="QAAGAAVPCGCGCHVPPAFDRAAAENSIMDAVDDDLANNPALPA
                     YQDKVAALSGAIDNAMDSIPSMPLDQLSQLAQQATDARNGLEDELKDAMLPAVQDAMD
                     EANAAKAAAQQAAAGAMSQAGSYEDQAQQAADGAMATARSTADGAMGTATSTADTAKS
                     MADQAASTASAAADAARQQAMQQIQDAARPASDAADLAQQTAQHLANQADDAVKQYLP
                     STPGMPSLPAGVPAIPAFG"
     gene            782..1618
                     /gene="ppk2"
                     /locus_tag="VF453_05645"
     CDS             782..1618
                     /gene="ppk2"
                     /locus_tag="VF453_05645"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX7687165.1"
                     /translation="MSKKKHDKGARDAHDDDAGAAGHGHHDKYEERLRPLQVRLNGVM
                     RTLASRGTRVVVLFEGRDSAGKGGSIKAISEKLNTRQVRTVALGVPSERERTQWYFQR
                     YVPHLPAAGEMVLFDRSWYNRAGVEAVMGFCTPDEVRAFLKAAPTFERMLVDDGIQVV
                     KYWLTVDQAEQEHRFAERLDDPLKQWKLSPVDLQARARYEEYGRARDRMLEATHTKWA
                     PWTLVDFNDQHEGRITLIEDLLSRLPAPEDPPPDFEIPPLGHEPLKEKFRGPLKPIKP
                     AP"
     gene            1662..2750
                     /gene="hemE"
                     /locus_tag="VF453_05650"
     CDS             1662..2750
                     /gene="hemE"
                     /locus_tag="VF453_05650"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX7687166.1"
                     /translation="MWPTLQNDTFLRACLRQPTDHTPVWLMRQAGRYLPEYRATRDRA
                     GSFMGLATNVELATEVTLQPLERFPLDAAILFSDILTVPDAMGLGLTFAAGEGPRFER
                     PLRDEADIAALAVPPLERLRYVFDAVSAIRRALGGRVPLIGFSGSPWTLACYMVEGKG
                     SDDYRLVKTLMYARPDLMHRVLAVNADAVAAYLNAQIDAGAQAVMIFDSWGGVLADAA
                     FHAFSLAYTRRVLAQLQREKDGARVPAIVFTKGGGQWLEAIADSGADVVGLDWTTDLG
                     AARRRVGDRVALQGNLDPTVLFAPPEVVQAQARAVLDSFGPPKRGDGGWDGHIFNLGH
                     GISQHTPPEHVTALVEAVHAHSSRRQAA"
     gene            3134..>3336
                     /locus_tag="VF453_05655"
     CDS             3134..>3336
                     /locus_tag="VF453_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687167.1"
                     /translation="MNHVGVAIDAPQHAGLGALLTYESPVPLAPGSLARVPLGRREVT
                     GVVWECMPAADAPPPAASPDPAAP"
BASE COUNT          533 a         1211 c         1154 g          438 t
ORIGIN      
        1 caggccgccg gcgcggcggt gccctgcggc tgcggctgcc acgtcccgcc ggcgttcgac
       61 cgcgccgcgg ccgagaactc gatcatggac gcggtcgacg acgacctcgc gaacaacccg
      121 gcgctgcccg cctaccagga caaggtcgcc gcgctgtccg gcgcgatcga caacgcgatg
      181 gacagcatcc cctcgatgcc ccttgaccag ctctcgcagc tcgcgcagca ggccaccgac
      241 gcgcgcaacg gcctcgagga cgagctcaag gacgcgatgc tgcccgcggt gcaggacgcg
      301 atggacgagg ccaacgccgc gaaggccgct gcgcagcagg ccgccgcggg cgcgatgagc
      361 caggccgggt cgtacgagga ccaggcgcag caggccgccg acggcgccat ggcgacggcc
      421 cggtcgaccg ccgatggcgc gatgggcacc gcgacgtcga ccgcggacac cgcgaagtcg
      481 atggccgacc aggcggcgag cacggcctcg gcggcggccg acgcggcgcg gcagcaggcg
      541 atgcaacaga tccaggacgc cgctcgcccg gcgagcgacg cggccgacct cgcgcagcag
      601 accgcgcagc acctcgccaa ccaggccgac gatgccgtca agcagtacct gccgtcgacg
      661 cccgggatgc cgtcgctgcc ggccggcgtg ccggcgatcc cggcgttcgg ctgaccccgc
      721 ctcgccgcgc ccgcgggcgc gccgaatgcc ggagggcgcg atctgggcgc atcatccggc
      781 catgagcaag aagaagcacg acaagggcgc gcgcgacgcg catgacgacg acgcaggggc
      841 cgccggtcac ggccaccacg acaagtacga ggagcgcctg cggccgctgc aggtgcggct
      901 caacggcgtc atgcgcacgc tcgcgagccg cgggacgcgc gtggtcgtgc tgttcgaagg
      961 ccgcgacagc gcgggcaagg gcggctcgat caaggcgatc tccgagaagc tcaacacgcg
     1021 ccaggtacgc accgtggccc tgggcgtgcc gagcgagcgc gagcgcacgc agtggtactt
     1081 ccagcgctac gtgccgcacc tgccggccgc cggcgagatg gtgctgttcg atcgcagctg
     1141 gtacaaccgc gccggcgtcg aggccgtgat gggcttctgc acgccggacg aagtgcgggc
     1201 cttcctcaag gcggcgccga cgttcgagcg catgctggtc gacgacggca tccaggtcgt
     1261 gaagtactgg ctcaccgtcg accaggccga gcaggaacac cgcttcgccg agcgcctgga
     1321 cgatccgctc aagcagtgga agctttcgcc cgtcgacctg caggcgcgcg cccgctacga
     1381 ggagtacggc cgcgcccgcg accgcatgct ggaagcgacg cacaccaagt gggcgccgtg
     1441 gacgctggtc gacttcaacg accagcacga aggccgcatc acgctgatcg aggacctcct
     1501 ttcgcgcctg ccggcgcccg aggatccgcc gcccgacttc gagatcccgc cgctcggcca
     1561 cgagccgctg aaggagaagt tccgcggccc cttgaagccg atcaagccgg cgccttgacc
     1621 tcgcgcgtgc ggacaggccg cgcgacggga taatccgtcg catgtggccc accctccaga
     1681 acgacacctt cctgcgcgcc tgcctgcgcc agccgaccga ccacacgccc gtctggctga
     1741 tgcgccaggc cgggcgctac ctgcccgagt accgcgccac gcgcgaccgc gccggcagct
     1801 tcatgggact ggccacgaac gtcgagctgg ccaccgaggt caccctgcag ccgctggagc
     1861 gtttcccgct cgacgcggcg atcctgttct cggacatcct caccgtgccg gacgcgatgg
     1921 gcctgggctt gacgttcgcg gcgggcgagg gcccgcgctt cgagcgcccg ctgcgcgacg
     1981 aggccgacat cgcggcgctg gcggtgccgc cgctggagcg gctgcgctac gtcttcgacg
     2041 cggtctcggc gatccggcgc gcgctcggcg gccgcgtgcc gctgatcggc ttctcgggca
     2101 gcccgtggac gctcgcgtgc tacatggtcg agggcaaggg ttcggacgac taccggctcg
     2161 tgaagacgct gatgtacgcg cggcccgacc tgatgcaccg cgtgctggcc gtcaacgccg
     2221 atgcggtcgc cgcctacctg aacgcccaga tcgatgccgg cgcgcaggcc gtgatgatct
     2281 tcgacagctg gggcggcgtg ctcgccgacg cagctttcca cgcgttctcg ctcgcctaca
     2341 cgcgccgggt gctggcgcag ctgcagcgcg agaaggacgg cgcacgcgtc cccgccatcg
     2401 tcttcaccaa gggcggcggc cagtggctgg aggcgatcgc cgacagcggc gcggacgtcg
     2461 tcgggctcga ctggaccacc gacctcggcg ccgcgcgccg ccgcgtgggc gaccgcgtcg
     2521 cccttcaggg caacctcgac ccgacggtgc tcttcgcgcc gcccgaggtc gtgcaggcgc
     2581 aggcgcgcgc cgtgctggac agcttcggcc cgcccaagcg aggcgacggt ggctgggatg
     2641 gccacatctt caacctcggc cacgggatca gccagcacac gccgcccgag cacgtcactg
     2701 ccctggtgga ggcggtgcac gcgcactcca gccgccgaca agcggcgtag gacgccactg
     2761 acaagtcacg aacgttgcaa cggcggtatt tgtccggtaa gtgagcgctc aggtgttgac
     2821 ttatccccaa aattcagact ccgacaagcc ggcctccgag gcacttgaca aaggcgccgg
     2881 agatcacccg ttttggtgcc aagtcattga tttgtttgaa tttttggggc tgcataaatt
     2941 ttgggcgatc cgacgcggcc gacggtgaat caagcgcttg gcggcggttc tgccagcttt
     3001 cccacaaagt tatccacaga aactgtgggt gacttcaaaa tgccatacgg atcatgagct
     3061 tacggctcgc agctcatacg ggccacaagc tttcgtcctc aagtgacagc cgcgtgacag
     3121 cggaaaggcg tccgtgaacc acgtcggcgt cgcgatcgac gcgccccagc acgccgggct
     3181 gggcgcgttg ctgacctacg agagccccgt gccgctcgcg cccggctcgc tcgcacgcgt
     3241 accgctgggc cggcgagaag tcacgggcgt cgtgtgggag tgcatgcccg cggccgacgc
     3301 gccgccgccg gccgcatcgc ccgacccggc cgcgcc
//
LOCUS       DASVLN010000245         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_334537, whole genome shotgun sequence.
ACCESSION   DASVLN010000245 DASVLN010000000
VERSION     DASVLN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334537"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VF453_05660"
     CDS             complement(<1..426)
                     /locus_tag="VF453_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7687168.1"
                     /translation="MSSTLCSDRPAGVDRAAHAPPPPAAAHWPAIASLALGVFGLVTA
                     EFLPASLLTAMSADLGVSDGAAGQAVTATALVGAIAAPTVPLATRRLDRRHVMLALTV
                     LLLASNALAIAAHGLPLLLAARVLLGVALGGFWSMAAALA"
     gene            503..1498
                     /locus_tag="VF453_05665"
     CDS             503..1498
                     /locus_tag="VF453_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7687169.1"
                     /translation="MLDHSSRPVRPPSSDFSKPLDPLTQVLLGLRLEGVEYGRCLLRT
                     PWAVAFKEQPHARFHFVARGGSWLRTRTGDWMRLDPGSAVLLPRGSFHVIASSPEVPA
                     IDIESLARRAVAEDIYLVEGDPKAAGADAPPPDVMFCGAMRFNLDPLHPLVAMMPEAM
                     RADGLAVRDPTVPALLEAMEREVELDRIGACGILSRLADVLAASIIRAWVECGCSDST
                     GWVAAVRCPKLGKVVAAIHADPARNWTVPMLAALMGASRSSFAEAFTRTLGESPAKYV
                     AKMKMVQARRWIAQDGMRVAVAADRLGYDSEASFSRAFKRVIGHSPSAARATAAR"
     gene            complement(1529..>2845)
                     /locus_tag="VF453_05670"
     CDS             complement(1529..>2845)
                     /locus_tag="VF453_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX7687170.1"
                     /translation="AHYERATALAPGFAAAHFGLGNTCQDAGRLEDAVACYQAALAAQ
                     PDHVEALNNLGVCQRQLGAIEEAAAAFERVLALRPDQIEAHCNLSPLKKYRPGDPQVD
                     RLLGLGYRVPALPEHGRVRYWFAAGKMLDDVGRHDEAFDAFAEGNRLKRALAPWDEAG
                     HDDLVRRIRATLGRETLAALAPDADEPADGPIPVFIVGMPRSGTSLLEQVLATHPAVH
                     GAGELTWLGEALASAAGDLAPGAEFAFPETLAAWPPEDLRRLGRRYRERLRALAPGAS
                     HVVDKLPGNFLHVGFMHVLFPDARIVHARRDPMDACFSCWSRLFAADHLAFTYDLEVL
                     GRYWVTYDDLMRHWRAALPPGRMLEMPYETMVADFEAQARRLVDYLGLPWDARCLEFH
                     DNRRVVRTASVAQVRRPIYRTSVERWRPYERRLAPLRAIVGGRYPA"
BASE COUNT          391 a         1008 c         1094 g          352 t
ORIGIN      
        1 cgcgagcgcc gccgccatcg accagaagcc gccgagcgcg acgcccagca gcacccgcgc
       61 ggccagcagc agcggcaggc cgtgggcggc gatcgcgagg gcgttcgacg ccaacagcag
      121 gacggtcagc gcgagcatca cgtgacggcg atccaggcgg cgcgtggcca gcggcacggt
      181 gggggcggcg atcgcaccga ccagcgccgt cgcggtgacc gcctggccgg cggcgccgtc
      241 gctgacgccg aggtcggccg acatcgccgt cagcaggctc gcgggcagga attcggcggt
      301 gacgaggccg aagacgccca acgccagcga ggcgatggcg ggccagtgcg ccgcggccgg
      361 cggtggcggg gcgtgggcgg cgcggtcgac gccggccggg cggtcggaac agagcgtgga
      421 ggacacggca agacgtttcc ggtgacaggg gaacgccagc gtaggtctaa gatgccggcg
      481 tttccatgcc ggaaaatctg aaatgcttga ccattcgtcc aggcccgtgc gaccgccctc
      541 gtccgacttc agcaagccgc tcgacccgct cacgcaggtg ctgctggggc tgcggctcga
      601 aggcgtcgag tacggccgct gcctgctgcg caccccctgg gcggtcgcgt tcaaggagca
      661 gccgcatgcg cgcttccact tcgtcgcccg cggcggcagt tggctgcgca cgcgcaccgg
      721 cgactggatg cgcctggacc ccggcagcgc ggtgctgctg ccgcgcggca gcttccacgt
      781 gatcgcgagc tcgcccgagg tgccggccat cgacatcgaa tcgctcgccc gacgcgccgt
      841 cgccgaggac atctacctcg tcgagggaga ccccaaggcc gcaggcgcgg atgcaccgcc
      901 gccggacgtg atgttctgcg gcgcgatgcg cttcaacctc gacccgctgc atccgctggt
      961 ggcgatgatg cccgaggcga tgcgcgccga cggcctcgcg gtgcgcgacc cgaccgtgcc
     1021 cgcgctgctc gaagcgatgg agcgcgaggt cgagctcgac cgcatcggcg cctgcggcat
     1081 cctgtcgcgg ctcgcggacg tgctggcggc cagcatcatc cgcgcctggg tggagtgcgg
     1141 ctgcagcgac tccaccggct gggtcgcggc ggtgcggtgt cccaagctcg gcaaggtggt
     1201 cgccgcgatc cacgcggatc cggcgcgcaa ctggacggtc cccatgctcg cggcgctgat
     1261 gggcgcgtcg cgctcgagct tcgccgaggc cttcacgcgc accttgggcg agagtccggc
     1321 gaagtacgtc gcgaagatga agatggtgca ggcgcgccgg tggatcgcgc aggacggcat
     1381 gcgcgtggcg gtcgcggccg accggctcgg ctacgactcc gaggcttcgt tcagccgcgc
     1441 gttcaagcgc gtgatcgggc attcgccgag cgcggcgcgc gcgaccgcgg cgcgctgagc
     1501 cgctcgcacg cccgcgcgat tgcctccttc acgccgggta gcgcccgccg acgatcgccc
     1561 gcagcggcgc cagccggcgc tcgtatggcc gccagcgctc gaccgaggtg cggtagatcg
     1621 gccgccgcac ctgggcgacg ctggcagtgc gcacgacgcg gcggttgtcg tggaactcga
     1681 ggcagcgcgc gtcccagggc aggccgaggt agtcgaccag gcgccgcgcc tgggcctcga
     1741 agtcggcgac catcgtctcg tagggcatct ccagcatgcg gccgggcggc agcgcggcgc
     1801 gccagtggcg catcaggtcg tcatacgtca cccagtatcg gccgagcacc tccaggtcgt
     1861 aggtgaaggc caggtggtcc gccgcgaaca gccgcgacca gcacgagaag caggcatcca
     1921 tcgggtcgcg ccgggcgtgc acgatgcgcg catcggggaa cagcacgtgc atgaagccga
     1981 cgtgcaggaa gttgcccggc agcttgtcga cgacgtgcga cgcgccgggc gcgagcgcgc
     2041 gcaggcgctc gcggtagcgg cggcccaggc ggcgcaggtc ctcggggggc caggcggcga
     2101 gggtctcggg gaacgcgaac tccgcgcccg gcgcaaggtc gccggccgcc gaggccagcg
     2161 cctcgccgag ccacgtcagc tcgcccgcgc cgtgcacggc cggatgggtg gcgagcacct
     2221 gctccagcag cgacgtgccc gaacgcggca tgccgacgat gaagacgggg atcggcccgt
     2281 ccgcgggctc gtccgcgtcc ggcgcgagcg ccgccagcgt ctcgcgcccg agcgtcgcgc
     2341 ggatgcggcg cacgaggtcg tcgtggccgg cctcgtccca tggggcgagc gcgcgcttga
     2401 ggcggttgcc ttcggcgaac gcgtcgaagg cctcgtcgtg gcggccgacg tcgtcgagca
     2461 tcttgcccgc cgcgaaccag tagcgcacgc ggccgtgctc ggggagcgcg gggacgcggt
     2521 agcccagccc gagcaggcgg tcgacctgcg ggtcgccggg ccggtacttc ttcaacggcg
     2581 acaggttgca atgggcctcg atctggtcgg gccgcagcgc gagcacgcgc tcgaaggccg
     2641 cggccgcctc ctcgatcgcg ccgagctggc gctggcagac gcccaggttg ttgagcgcct
     2701 cgacgtgatc cggctgcgcg gcgagcgcgg cctggtagca ggcgacggcg tcctcgaggc
     2761 ggccggcgtc ctggcaggtg ttgcccaggc cgaagtgcgc cgccgcgaag cccggggcga
     2821 gcgccgtcgc gcgctcgtag tgcgc
//
LOCUS       DASVLN010000246         6769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_442736, whole genome shotgun sequence.
ACCESSION   DASVLN010000246 DASVLN010000000
VERSION     DASVLN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442736"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VF453_05675"
     CDS             complement(<1..244)
                     /locus_tag="VF453_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687171.1"
                     /translation="MDEARDVSGERRARVAYTCGMEREATAMPPGRTRCEGPRAPRGL
                     AAAGAGIVLAAAVAAAGASVHHRAPAAKRARVAAAST"
     gene            254..1726
                     /locus_tag="VF453_05680"
     CDS             254..1726
                     /locus_tag="VF453_05680"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX7687172.1"
                     /translation="MIEPLLVPRESFDDPDAAVERVRQIYQAGVDHLRRHLHEFVAGR
                     VPAAHVRACYPSVRVHTDTVARADSVSSYGFVAGPGTFETTLTRPELFASYYREQFRL
                     LRQHHGVKVEVGVSREPMPLHFSFAEHDHVEGTLTAQQRLQLRDLFDLPNLAAMDDGI
                     ANGTHDPGAGEPLPLALFTAPRVDYSLHRLRHYTGTAPEHFQNFVLFTNYQFYIDEFI
                     ALGHREMARADSDYDAFVEPGNVVTARAGRPAPAGPAGIAPQRLPQMPAYHLKRSDNS
                     GITMVNIGVGPSNAKNISDHIAVLRPHAWIMLGHCAGLRTTQQLGDYVLAHGYVREDH
                     VLDEELPLWVPIPPLAEVQVALEQAVEQITQLSGMALKRVMRTGTVASTDNRNWELLP
                     HPGPERRFSQSRAIALDMESAAIAANGFRFRVPYGTLLCVSDKPLHGEIKLPGMANHF
                     YRERVEQHLRIGIRALELLRDAGAKLHSRKLRGFAEVAFQ"
     gene            1746..2621
                     /locus_tag="VF453_05685"
     CDS             1746..2621
                     /locus_tag="VF453_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HEX7687173.1"
                     /translation="MFRTLVQPGRPEAQRVESVEGPALEAAFVVPAGATLLEGIAAGM
                     ARAGLRCGTVRLADVEVASLQYVRPAPAADGEHVAFYSATHRIEGPLRLQRVVATFGE
                     KDGRPFVHAHGLWFDERGRLAGGHLHNDAVRVAADAPARAWGLAGAAMAVEFDEETRF
                     SLFRPRPVGDAAPADAGMPRCVIARVRPNEDLLAAVERTAAAHGIASAHIAGSVGSLV
                     GASFADGREVADTATEILVLHGAVEPASGTPRATLEIALVDPRGDVHVGVLARGANAV
                     LICFELVLVEDRGAT"
     gene            2787..4838
                     /gene="fusA"
                     /locus_tag="VF453_05690"
     CDS             2787..4838
                     /gene="fusA"
                     /locus_tag="VF453_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7687174.1"
                     /translation="MTSLDTSDIAAIRNVALVGASATGKTTLAEALLARAGAIKTIGS
                     VERGSTVSDHDPLERRAQHSLSTSILNLEHDGARVQLLDTPGALEFVGQSLPALEAVE
                     TAAIVISAAAGVDATAQRMMAHAAERKLDRMIVVNRIDVAGADCAAVLAQIRETFGRE
                     CLPLNLPAAGGTRVVDCFFNRGGETDFSSPEEAHRALVEQVVEVDADFVDRYLNQGDV
                     DARELHAPLEQALREGHLIPVCFVSARTGAGVPELLDAIAKLLPNPSEGNPPDFLDGE
                     GADARLVHARPDPSMHVLAHVFKVAIDPYVGKMGVLRVHQGTITRDSQLFVGDGRKPF
                     KVGHLFRLQGAEHVEIPRAGPGDICAIAKVDALHFDAVLHDAAEDSHIHLKALSFPTP
                     VHGIAIEPKRHGDEQRLWDLVQRAVDEDPCLRIEQVAATRETVVNGLGELHLRMLLER
                     LREVHKFEVETRPPRVAYRETITAKAEGHYRHKKQTGGAGQFGEVFLRVEPLGRGEGF
                     EFVDVVKGGAIPGQFMQAVEKGVREALAAGAIAGYPVEDIRVTIHDGKSHSVDSKDIA
                     FATAARKATWVAIMEARPIVLEPIVDVEITAPAAAMGDITGDLASRRGQVSGTDALLG
                     GAIVVRGKAPAAELIGYAMRLNAMTSGEGRFTVAFSHYEPAPASVQNALRTQHKPVEV
                     D"
     gene            complement(4927..5472)
                     /locus_tag="VF453_05695"
     CDS             complement(4927..5472)
                     /locus_tag="VF453_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687175.1"
                     /translation="MKKIQALSRFASIAALCAAAAVMAGCATASKPENMIAKPAVAGI
                     HHPQTTSVVVAGGSETSSMGKSQISNDAFRQAIAASIEQSKTFSAVVEGKGGDYQLGV
                     TVISMDQPSFGLSFTVKMEAAWSLKKADGTAVWQDSIKSEGTATTGDAFAGVERLRIA
                     NEKAAQANIAAALGKIGQLQF"
     gene            complement(5564..6001)
                     /locus_tag="VF453_05700"
     CDS             complement(5564..6001)
                     /locus_tag="VF453_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687176.1"
                     /translation="MFRRIAAAGSVALVLGATTGCAVNQAQSTLMAGADLGKVKSIYV
                     VHEEGDDHAVDLELKKAFEKRGYTVATGPALKPPYEQDAVVTYVDKWMWDITMYMLQL
                     NVTMRDSKEFPLATAHSVHTSLTRKSQPEMVDEVVTNVLAAKN"
     gene            complement(6118..>6769)
                     /locus_tag="VF453_05705"
     CDS             complement(6118..>6769)
                     /locus_tag="VF453_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687177.1"
                     /translation="DPGGGRRRAAWLLALAVLAAAWAGFDLWLSSVPGESGTAMTGMY
                     LAAPLGLGIWCGLWALVSKIFQRQFAFWPHLEAALFWPLVATIVEAVCGQAAFALSLP
                     VLAKAGHVLAIAAITMLLWRHLSIVQPTRRRAFGWVLVGVVAVVGGLNVAERVRHQQP
                     LVGGLYLGTISLPAMRLAKPESADAFVQSAAPLQKSLSRWAKKGDDDEQEGAPDDD"
BASE COUNT          967 a         2497 c         2369 g          936 t
ORIGIN      
        1 gcgtcgacgc ggcggcgacc ctggcccgct tcgcggccgg cgcacggtga tgcacgctcg
       61 cgccggccgc ggccaccgcc gcggcgagca cgatcccggc gcccgccgcc gcgaggccgc
      121 gcggcgcgcg cgggccctcg catcgcgtcc gtccgggggg catcgccgtg gcttctcgtt
      181 ccatcccgca agtgtaggca acgcgcgcgc ggcgctcacc ggaaacgtca cgcgcttcgt
      241 ccacaatggc cgcatgatcg aaccgctgct cgtgccccgc gaatccttcg acgaccccga
      301 cgccgcggtg gaacgcgtgc gccagatcta ccaggccggc gtcgaccacc tgcgccgcca
      361 cctgcacgag ttcgtcgccg ggcgcgtgcc cgcggcgcac gtgcgcgcct gctacccgag
      421 cgtgcgcgtg cacaccgaca cggtcgcgcg cgccgactcc gtctccagct acggcttcgt
      481 cgcgggcccc ggcacgttcg agaccacgct cacgcgcccg gagctcttcg cgtcgtacta
      541 ccgcgagcag ttccgcctgc tgcggcagca ccacggcgtg aaggtcgagg tcggcgtcag
      601 ccgagagccc atgccgctgc acttcagctt cgccgagcac gaccacgtcg agggcacgct
      661 gacggcgcag cagcgcctgc agctgcgcga cctgttcgac ctgcccaacc tcgcggcgat
      721 ggacgacggc atcgccaacg gcacgcacga ccccggcgcg ggcgagccgc tgccgctcgc
      781 gctgttcacg gcgccgcgcg tcgactactc gctgcaccgc ctgcgccact acaccggcac
      841 ggcgcccgag cacttccaga acttcgtcct gttcacgaac taccagttct acatcgacga
      901 gttcatcgcg ctgggccacc gcgagatggc gcgcgcggac tccgactacg acgccttcgt
      961 cgagcccggc aatgtcgtca cggcgcgcgc cggccgaccg gcgccggccg gacccgcggg
     1021 gatcgcgccg cagcgcctgc cgcagatgcc ggcgtaccac ctcaagcgct cggacaacag
     1081 cggcatcacg atggtcaaca tcggcgtcgg gccgtccaac gcgaagaaca tcagcgacca
     1141 catcgccgtg ctgcgcccgc acgcgtggat catgctgggc cactgcgcgg ggctgcgcac
     1201 gacgcagcag ctcggcgact acgtgctggc ccacggctac gtgcgcgagg accatgtgct
     1261 cgacgaggag ctgccgctgt gggtgccgat cccgccgctc gccgaagtgc aggtggcgct
     1321 cgagcaggcg gtggagcaga tcacgcagct gtccggcatg gcgctcaagc gcgtgatgcg
     1381 cacgggcacc gtcgcctcga ccgacaaccg caactgggag ctgctgccgc atccgggccc
     1441 ggagcgccgc ttcagccaga gccgcgcgat cgcgctcgac atggaatccg ccgccatcgc
     1501 cgccaatggc ttccgcttcc gcgttccgta cgggacgctg ctgtgcgtct ccgacaagcc
     1561 gctgcacggc gagatcaagc tgcccggcat ggccaaccac ttctaccgcg aacgcgtcga
     1621 gcagcacctg cgcatcggca tccgcgcgct cgagctgctg cgcgatgccg gcgcgaagct
     1681 gcacagccgc aagctgcgcg ggttcgcgga ggtcgcgttc caatgacgcc ccgcgcgcgc
     1741 tgaccgtgtt caggacgctc gtccagcccg gccggcccga agcgcagcgc gtcgagtcgg
     1801 tcgaaggccc cgcgctcgaa gccgcgttcg tcgtgcccgc cggcgcgacg ctgctcgaag
     1861 gcatcgccgc cggcatggcg cgcgcgggcc tgcgctgcgg cacggtgcgg ctggcggacg
     1921 tcgaggtcgc ctcgctgcag tacgtgcgcc cggcaccggc cgccgacggc gagcatgtcg
     1981 ccttctacag cgcgacccac cgcatcgagg ggccgctgcg gctgcagcgt gtcgtcgcca
     2041 ccttcggcga gaaggacggc cggccgttcg tgcatgcgca cggcctctgg ttcgacgaac
     2101 ggggccggct cgcgggcggc catctgcaca acgacgcggt gcgcgtggcc gcggacgcgc
     2161 ccgcacgcgc gtggggcctg gccggggcgg cgatggcggt cgagttcgac gaggagacgc
     2221 gcttcagcct gttccgtcct cggcccgtcg gcgacgccgc cccggccgac gccggcatgc
     2281 cccgctgcgt gatcgcgcgg gtgcggccca acgaggacct gctggccgcc gtcgagcgca
     2341 cggccgcggc gcacggcatc gcatcggcgc acatcgcggg cagcgtgggc agcctggtcg
     2401 gcgcgagctt tgccgacggc cgcgaggtcg ccgacacggc caccgagatc ctcgtgctgc
     2461 acggtgcggt cgagcccgcg tcgggcacgc cgcgcgccac gctcgagatc gcgctggtcg
     2521 atccgcgcgg cgatgtccac gtcggcgtgc tcgcgcgcgg cgccaacgcg gtgctgatct
     2581 gcttcgagct cgtcctcgtc gaggatcgcg gggcgacctg agcgggtcgc gatggggagc
     2641 gtcacgcgac ccgccaggca gtgcgccgac ccttgcaccg acccgcccac gggcgtaggc
     2701 tgcggcttca tcctgccatc cggccgtgac gcggttcggg tcgccgcgga gccggtctgg
     2761 cgtctcggcc cgtggagaca cgtgccatga cgagcctgga cacatcggac attgcagcga
     2821 tccgcaacgt cgccctggtg ggcgcctcgg ccaccgggaa gacgaccctc gccgaggcct
     2881 tgctggcgcg cgcgggcgcc atcaagacga tcggctccgt cgagcgcggc tcgaccgtga
     2941 gcgaccacga tccgctggaa aggcgcgccc agcactcgct gtcgaccagc atcctcaatc
     3001 tcgagcacga cggcgcccgc gtgcagttgc tcgacacgcc cggggcgctc gagttcgtcg
     3061 gccagtcgct gccggcgctc gaggccgtgg agacggcggc catcgtcatc agcgcggctg
     3121 ccggcgtcga cgcgaccgcg cagcgcatga tggcgcacgc ggccgagcgc aagctcgatc
     3181 gcatgatcgt cgtcaaccgc atcgacgtcg ccggcgcgga ctgcgccgcc gtgctcgccc
     3241 agatccgcga gaccttcggc cgcgaatgcc tgccgctgaa cctgcccgcc gccggcggca
     3301 cgcgcgtcgt cgactgcttc ttcaaccgcg gcggcgagac cgacttctca tcgccggagg
     3361 aagcgcaccg cgcactcgtc gagcaggtcg tggaggtcga cgccgatttc gtcgaccgct
     3421 acctgaacca gggcgacgtc gacgcgcgcg agctgcacgc gccgctggag caggcgctgc
     3481 gcgaaggtca cctgatcccg gtgtgcttcg tctccgcgcg caccggtgcc ggcgtgcccg
     3541 agctgctgga cgcgatcgcg aagctgctgc ccaatccgag cgagggcaac ccgcccgact
     3601 tcctcgacgg cgagggcgcc gacgcgcgcc tcgtgcatgc gcggcccgac ccgtcgatgc
     3661 acgtgctcgc gcatgtcttc aaggtcgcga tcgaccccta cgtcggcaag atgggcgtgc
     3721 tgcgcgtgca ccagggcacg atcacgcgcg acagccagct gttcgtcggc gacggccgca
     3781 agcccttcaa ggtcggccac ctgttccggc tgcagggcgc cgagcacgtc gagatcccgc
     3841 gcgccggccc gggcgacatc tgcgccatcg ccaaggtcga cgcgctgcac ttcgatgccg
     3901 tgctgcacga cgccgcggag gacagccaca tccacctgaa ggccctgtcc ttcccgacgc
     3961 cggtgcacgg catcgcgatc gagccgaagc gccacggcga cgagcagcgc ctctgggacc
     4021 tcgtgcagcg cgcggtcgac gaggacccgt gcctgcgcat cgagcaggtc gcggccacgc
     4081 gcgagacggt ggtcaacggc ctgggcgagc tgcacctgcg catgctgctg gagcggctgc
     4141 gcgaggtgca caagttcgag gtggagacgc gccctccgcg cgtcgcctac cgcgagacga
     4201 tcaccgcgaa ggcggagggc cactaccgcc acaagaagca gaccggcggc gccggccagt
     4261 tcggcgaggt cttcctgcgc gtcgagccgc tcggccgcgg agagggcttc gagttcgtcg
     4321 acgtcgtcaa gggcggcgcg atcccgggcc agttcatgca ggcggtcgag aagggcgtgc
     4381 gcgaggcgct ggccgccggc gcgatcgcgg gctatccggt cgaggacatc cgcgtcacga
     4441 tccacgacgg caagagccac agcgtcgact cgaaggacat cgccttcgcg accgcggcgc
     4501 gcaaggcgac ctgggtcgcc atcatggagg cgcggcccat cgtgctcgag cccatcgtcg
     4561 acgtcgagat caccgcgccc gccgccgcga tgggcgacat cacgggcgac ctcgcctcgc
     4621 ggcgcggcca ggtcagcggc accgacgcgc tcctgggcgg cgccatcgtc gtgcgcggca
     4681 aggcgccggc cgccgagctg atcggctacg cgatgcgcct gaacgcgatg accagcggcg
     4741 aagggcgctt cacggtcgcc ttctcccact acgagcccgc gcccgcgagc gtgcagaacg
     4801 cgctgcgcac gcagcacaag ccggtcgagg tcgactgagc agccgcctct cgacggacaa
     4861 ggggcgcgtg cccgtcgccg gacgcgcgcc ccttggtcct cgtggcgccg ccgcggcggc
     4921 gcgccgtcag aattgcagct ggccgatctt gcccagcgcg gcggcgatgt tggcctgggc
     4981 ggccttctcg ttggcgatgc gcaggcgctc gacgccggcg aacgcgtcac cggtcgtcgc
     5041 ggtgccttcc gacttgatgc tgtcctgcca gacggccgtg ccgtcggcct tcttcagcga
     5101 ccaggcggct tccatcttca ccgtgaagga caggccgaac gacggctggt ccatcgagat
     5161 gacggtcacg ccgagctggt agtcgccgcc cttgccctcg acgacggcgg agaacgtctt
     5221 gctctgctcg atcgacgcgg cgatggcctg gcggaaagcg tcgttcgaga tctgcgactt
     5281 gcccatcgag ctcgtctcgc tgccgccggc gacgacgacg ctggtcgtct gcggatggtg
     5341 gatgccggcg acggccggct tggcgatcat gttctccggc ttggaggcgg tggcgcagcc
     5401 ggccatgacg gcggcggccg cgcacagggc ggcgatggag gcgaaacggg acagcgcctg
     5461 gatcttcttc atgatgggat gtgtgttgac gtggttgcga tgaggcacgc cgatgccgcc
     5521 gtcggcgtgt ccggccagcg gcgtcggcgt gcgaggggaa cgctcagttc ttggcggcga
     5581 ggacgttggt gacgacctcg tccaccatct cgggctgcga cttgcgcgtg agcgaggtgt
     5641 gcacggaatg cgccgtggcg agcgggaact ccttcgagtc gcgcatcgtc acgttgagct
     5701 gcagcatgta catcgtgatg tcccacatcc acttgtcgac gtaggtcacg acggcgtcct
     5761 gctcgtacgg cggcttcagc gccgggccgg tggccacggt gtagccgcgc ttctcgaagg
     5821 ccttcttcag ctcgaggtcg accgcgtggt cgtcgccctc ctcgtggacg acgtagatcg
     5881 atttgacctt gcccaggtcg gcgcccgcca tgagcgtgga ctgggcctgg ttgacggcgc
     5941 agccggtggt ggcgccgagg acgagcgcga ccgagccggc cgcggcgatg cgtctgaaca
     6001 tgaactccct ccctgtgaac gtttgttgct cagcgggccg gattatttca gttcgtcgct
     6061 ccagctcgac agtggcaaat ccgagttcag ctcgcgttca ggttctcgcg gccgcggtca
     6121 gtcgtcgtcg ggcgcgcctt cctgctcgtc gtcgtcgccc ttcttcgccc agcgcgagag
     6181 cgacttctgc agcggcgccg ccgactggac gaacgcgtcg gccgactccg gcttcgccag
     6241 gcgcatcgcc ggcaacgaga tcgtcccgag gtagagcccg ccgaccagcg gctgctggtg
     6301 gcgcacgcgc tcggcgacgt tcaggccgcc gacgaccgcg accacgccga cgaggaccca
     6361 gccgaacgcg cgccgccgcg tcggctgcac gatgctcagg tggcgccaca gcagcatcgt
     6421 gatcgccgcg atcgccagca cgtgcccggc cttggccagc acgggcagcg acagggcgaa
     6481 cgccgcctgg ccgcagacag cctcgacgat cgtggccacc agcggccaga acagggccgc
     6541 ctccaggtgc ggccagaacg cgaactggcg ctggaagatc ttcgagacca gcgcccacag
     6601 cccgcaccag atgcccagcc cgagcggcgc cgcgaggtac atgccggtca tcgccgtgcc
     6661 cgactcgccc ggcaccgacg acagccagag gtcgaagccc gcccacgccg cggccagcac
     6721 cgcgagcgcg agcagccagg ccgcacgcct gcggccaccg ccggggtcg
//
LOCUS       DASVLN010000247         5512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_226666, whole genome shotgun sequence.
ACCESSION   DASVLN010000247 DASVLN010000000
VERSION     DASVLN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226666"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2271)
                     /locus_tag="VF453_05710"
     CDS             complement(<1..2271)
                     /locus_tag="VF453_05710"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX7687178.1"
                     /translation="MSIRSFAADLLSPKQHPPARTAPPPEPQAEVSTFAALSEKLDYL
                     SKLDLKKVREAYKFADEAHLGQLRASGIPYITHPLAVAGLVADWKLDAQALVAALTHD
                     TMEDCGVTKPELIERFGAPTADLVDGLTKLDKLQFSTREEGQAESFRKMLLAMSRDVR
                     VILIKLCDRLHNMRTMDAMAPGKRARIARETIEIYAPIAHRLGLNQMYRELQDLSFRF
                     IHPWRYAAVAKALERARGTRRDIVDRMRRDVEAAFAAARRRVTVYSREKTVFSIYRKM
                     REKHAGFAQVNDIFGFRVVVNSLDECYQSLGTLHQMYKPVPGRFKDYIAIPKANGYQS
                     LHTTLVNPLGTAVEFQVRTEQMHAVAEAGIAAHWMYKALSKEGRDGKGGPMPDEAQRL
                     GAMWLQSLIDIQRDTHDSSEFLEHVKIDLFPDAVYVFTPKSKILALPRGATPIDFAYA
                     IHSDVGDHCVAAKVNGEPVALRTELKSGDVVEVSTSPNARPNPSWLNSVRTGRARAKI
                     RHFLKNLEQEESIALGEKLLAQALRSEGMAMPVEDESNAAVWQALTRWSGSRSRAELM
                     VDIGLGRKISSIVAKRLAQLLAERGGRPDAVTLTMGRYGEGDGQPVHNLVEIDGSEGA
                     SVQLATCCRPIPGDEIAGHFGGEGLTVHTTDCSIGRRLKDRDAERWMGVDWAEVLTRP
                     FETSVTVLVRNAKGALAQVAQAISAAEADITHIDMGDERAAETTELRLLVSVRDRLHL
                     ADVLRTLKRAPVVLRAA"
     gene            2583..4118
                     /locus_tag="VF453_05715"
     CDS             2583..4118
                     /locus_tag="VF453_05715"
                     /inference="COORDINATES: protein motif:HMM:NF027798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 64 protein"
                     /protein_id="HEX7687179.1"
                     /translation="MTRLSIRLACPLAGLLVALAPAARAQTAADYAQGVTVAGSTATI
                     WFQPSSSSTTWTDVHYQLDGGPQQNLRMARDASTGRETEAVAAPVSAGSTLRYFFTYD
                     KGSPAYDSASYSYTVGSGSGSGSGSGSGSGSGGTWNQHTTFHLVNGTHGAYGDAQVYW
                     AIVGKDWATGRFVHVDAGGALVPMSTADNGALAKDGVAYTDYFHTLAQASTVTIPPID
                     SARLLLSVGGPMYLRVNQDAAGNLGYTGANIENPSDPNIDVTFDFVEMAIDPNSGFYG
                     NTTRVDQFGFPVTLRLQGLGGYDQTVGETETRAALVSEFAGAVPGPFQGLAQSPYLPY
                     RIVAPGHASFRPGQPNANYLDGYIATVWNEYQARTLTFTDQQGTFTGHVVNGAFVFTD
                     GQGTYTIARAPTTAEALLGNGVLNDPTGQSPGTPGWDKQLQIQAQLCAAINRHIVENP
                     SQWSTPGAFYGAGSVANWYARFWHDHALGGLAYGFPYDDVGGFSSSLHTAAPTTATVT
                     IGW"
     gene            complement(4249..4452)
                     /gene="rpoZ"
                     /locus_tag="VF453_05720"
     CDS             complement(4249..4452)
                     /gene="rpoZ"
                     /locus_tag="VF453_05720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX7687180.1"
                     /translation="MARITVEDCLEKIPNRFQLVLAATYRARMLSQGHAPKIDSKNKH
                     GVTALREIAAGEVGKEMLRKVPT"
     gene            complement(4496..5122)
                     /gene="gmk"
                     /locus_tag="VF453_05725"
     CDS             complement(4496..5122)
                     /gene="gmk"
                     /locus_tag="VF453_05725"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7687181.1"
                     /translation="MELPGKLFVVAAPSGAGKSTLVKALLQHEPQLAVSVSHTTRSPR
                     GQERDGREYHFVDTTTFRAMVDDGQFFEHAHVHGNWYGTSRRSVESLLEDGKDVVLEI
                     DWQGALQIRELFPAAKLIFILPPSWDTLALRLRGRGEDAPEVIAQRLDNARHEVAQAP
                     LFDYVIINGSIDAAVADLRAVVRDQPERLCFAAQRDAHPEVFAALQLP"
     gene            complement(5235..>5512)
                     /locus_tag="VF453_05730"
     CDS             complement(5235..>5512)
                     /locus_tag="VF453_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HEX7687182.1"
                     /translation="RAHVFYDTGFDLHSRRQFCSRYVREVLQEGAGVQVGRIETFAGL
                     LHESPGADLGFWRAWYFGRIPWQRETVTPASLLRSPGLTTVFDGTAA"
BASE COUNT          787 a         1901 c         1969 g          855 t
ORIGIN      
        1 ggccgcgcgc aggacgaccg gcgcgcgctt gagcgtgcgc agcacgtcgg ccaggtgcag
       61 gcggtcgcgc acgctcacga gcaggcgcag ctcggtggtc tcggcggcgc gttcgtcgcc
      121 catgtcgatg tgcgtgatgt cggcctcggc ggcgctgatc gcctgcgcga cctgcgccag
      181 cgcgcccttg gcgttgcgca ccaggacggt caccgaggtc tcgaacgggc gcgtgagcac
      241 ctccgcccag tcgacgccca tccagcgctc ggcgtcgcgg tccttgaggc ggcggccgat
      301 cgagcagtcc gtcgtgtgca cggtcaggcc ctcgccgccg aagtggccgg cgatctcgtc
      361 gcccgggatc ggccggcagc aggtcgccag ctgcaccgac gcgccttcgc tgccgtcgat
      421 ctcgacgagg ttgtgcacgg gctgcccgtc gccctcgccg tagcggccca tcgtcaaggt
      481 gacggcgtcg gggcggccgc cgcgctcggc cagcagctgc gcgaggcgct tggcgacgat
      541 cgacgagatc ttgcgcccga ggccgatgtc gaccatcagc tcggcgcggc tgcggctgcc
      601 gctccagcgc gtgagcgcct gccagacggc agcgttggac tcgtcctcga ccggcatcgc
      661 catgccctcc gagcgcagcg cctgcgcgag cagcttctcg ccgagcgcga tcgactcctc
      721 ctgctcgagg ttcttcagga agtggcggat cttggcgcgc gcgcgtcccg tgcgcaccga
      781 gttcagccat gacgggttcg gccgcgcgtt gggcgaggtg ctgacctcga cgacgtcgcc
      841 gctcttcaat tcggtgcgca gcgccaccgg ctcgccgttg accttggccg cgacgcagtg
      901 gtcgccgacg tcggagtgga tcgcgtaggc gaagtcgatc ggcgtcgcgc cgcgcggcag
      961 cgcgaggatc ttgctcttgg gcgtgaagac gtagaccgcg tcggggaaca ggtcgatctt
     1021 cacgtgctcg aggaactcgc tcgagtcgtg ggtgtcgcgc tggatgtcga tcagcgactg
     1081 cagccacatc gcgcccaggc gctgggcctc gtcgggcatc gggccgccct tgccgtcgcg
     1141 gccctccttg gacagcgcct tgtacatcca gtgcgccgcg atgccggcct cggcgacggc
     1201 gtgcatctgc tcggtgcgga cctggaactc gaccgccgtg ccgagcgggt tgacgagcgt
     1261 cgtgtgcagc gactggtagc cgttggcctt ggggatcgcg atgtagtcct tgaagcggcc
     1321 cggcaccggc ttgtacatct ggtgcagcgt gccgagcgac tggtagcact cgtcgagcga
     1381 gttcacgacc acgcggaagc cgaagatgtc gttgacctgc gcgaacccgg cgtgcttctc
     1441 gcgcatcttg cggtagatcg agaagacggt cttctcgcgg ctgtagacgg tcacgcgccg
     1501 gcgcgcggcg gcgaaggcgg cctcgacgtc gcggcgcatg cggtcgacga tgtcgcgccg
     1561 cgtgccgcgc gcgcgctcga gcgccttggc caccgcggcg tagcgccagg ggtggatgaa
     1621 gcggaacgag agatcctgca gctcgcggta catctggttc aggcccaggc ggtgcgcgat
     1681 cggggcgtag atctcgatcg tctcgcgcgc gatgcgggcg cgcttgccgg gcgccatcgc
     1741 gtccatcgtg cgcatgttgt gcaggcggtc gcacagcttg atgaggatga cgcgcacgtc
     1801 gcgcgacatc gccagcagca tctttcggaa ggactcggcc tggccctcct cgcgcgtgga
     1861 gaactgcagc ttgtcgagct tggtcagccc gtcgacgagg tcggcggtgg gggcgccgaa
     1921 gcgttcgatc agctcgggct tggtgacgcc gcagtcctcc atcgtgtcgt gcgtcagcgc
     1981 ggccacgagc gcctgcgcgt cgagtttcca gtccgccacg aggccggcga cggccagtgg
     2041 gtgcgtgatg taggggatgc cgctcgcccg cagctggccg aggtgggcct cgtcggcgaa
     2101 cttgtaggcc tcgcgcacct tcttcaggtc gagcttgctg aggtagtcca gcttctcgga
     2161 cagcgccgcg aaggtggaca cctccgcctg cggctcgggc gggggggccg tgcgcgccgg
     2221 cggatgctgc ttcggcgaca gcaggtcggc ggcgaacgat cgaatgctca tcccggaaat
     2281 ttagcgtgta aaccgcccgc ccgacggcgg ccggccgaat gtccgcgcat tcctgcctcc
     2341 cgcagcacgt gcaggcgagc ctttcgatgg ctgccgggcg acggtcgacc gggtttgccc
     2401 tggaaatcgt tggcaataag tccatcgtat ttgcgaacat ctgtttgcaa atggcgagca
     2461 aatgaaactt ttccgcccca ggagccggca tcgtccctag catgcctccg gttcgcactg
     2521 gttctggaac cggttcccga ccagtcgagt cgcccatcgg ccacttccac ggagactccg
     2581 ccatgacacg cctgtccatc cgcctcgcct gcccgttggc gggcctgctc gtcgccctgg
     2641 cgcccgccgc ccgcgcgcag accgccgccg actatgcgca gggggtgacg gtcgccggca
     2701 gcaccgcgac gatctggttc cagccgagtt cgtcgtccac gacttggaca gacgtgcact
     2761 accagctcga cggcggcccg cagcagaacc tgcgcatggc ccgcgacgcg tccaccggcc
     2821 gcgagaccga ggccgtggcg gcgccggtgt ccgcgggcag cacgctgcgc tacttcttca
     2881 cctacgacaa ggggtcgccg gcgtacgact cggcgagcta cagctacacc gtcggctccg
     2941 gctccggctc cggctccggc tcgggatccg gttcgggctc gggcggcacc tggaaccagc
     3001 acaccacctt ccacctcgtc aacggcacgc acggcgcgta cggcgacgcg caggtctact
     3061 gggccatcgt gggcaaggac tgggcgaccg gccgtttcgt gcacgtggac gcgggcggcg
     3121 ccctggtgcc gatgtcgacc gccgacaacg gcgcgctggc caaggacggc gtcgcctaca
     3181 ccgactactt ccacacgctg gcgcaggcga gcacggtgac gatcccgccg atcgactccg
     3241 cgcgcctgct gctgagcgtg ggcggcccga tgtacctccg cgtgaaccag gacgccgcgg
     3301 gcaacctcgg ctacaccggc gccaacatcg agaacccgag cgaccccaac atcgacgtca
     3361 ccttcgactt cgtggagatg gccatcgacc cgaactcggg cttctacggc aacacgacgc
     3421 gcgtcgacca gttcggcttc ccggtcacgc tgcgcctgca ggggctgggc ggctacgacc
     3481 agacggtggg cgagacggag acgcgcgcgg cgctggtgtc ggagttcgcc ggtgcggtgc
     3541 cggggccgtt ccagggcctc gcccagtcgc cctacctgcc gtaccgcatc gtcgcgccgg
     3601 ggcacgccag cttccgtccc ggccagccga acgcgaacta cctcgacggc tacatcgcca
     3661 cggtctggaa cgagtaccag gcgaggacgc tgaccttcac cgaccagcag ggcaccttca
     3721 ccggccacgt cgtgaacggc gccttcgtct tcacggacgg ccagggcacc tacaccatcg
     3781 cgcgcgcgcc gacgaccgcc gaggccctgc tgggcaacgg cgtgctcaac gatccgacgg
     3841 ggcagtcgcc cggcacgccc ggctgggaca agcaactgca gatccaggcc cagctgtgcg
     3901 ccgcgatcaa ccgccacatc gtcgagaacc cgtcccaatg gagcacgccc ggggcgttct
     3961 acggcgccgg ctcggtcgcg aactggtacg ccaggttctg gcacgaccac gcgctcggcg
     4021 ggctggccta cggcttcccg tacgacgacg tcggcgggtt cagctcctcg ctgcacacgg
     4081 cggcgccgac gactgcgacc gtgacgatcg gctggtgagg cgccggacgc gccccgggcg
     4141 cgaatgaaaa gagcccgcgg ccgtgcggca ggcgggctct tcttgcgggc gcgctgtcag
     4201 gcgcgcttcc gcgccagcgc gcgcctcccg cgcgcgcggg gctgcggatc aggtcggaac
     4261 cttgcgcagc atctccttgc cgacttcgcc ggcggcgatc tcgcgcaggg cggtgacgcc
     4321 gtgcttgttc ttggagtcga tcttgggcgc gtggccctgg ctcagcatgc gggcgcgata
     4381 ggtcgcggcg agcacgagct ggaagcggtt ggggatcttc tccaggcagt cttcaacggt
     4441 gatgcgggcc atgaggggtt cctgggctag ggtgaggcgg tcgatcggtg cgcattcagg
     4501 gaagctggag cgcggcgaac acctcggggt gagcgtcgcg ctgggccgcg aagcacagcc
     4561 gctcgggctg gtcgcggacg acggcgcgaa ggtccgccac cgcggcgtcg atcgaaccgt
     4621 tgattataac gtagtcgaac agcggcgcct gggcgacctc gtggcgcgca ttgtccaggc
     4681 gctgggcgat gacctcgggc gcatcctcgc cgcggccgcg caggcgcagg gcgagcgtgt
     4741 cccagctcgg gggcaggatg aagatcagct tcgcggccgg gaacagctcg cggatctgca
     4801 gggcgccctg ccagtcgatc tccagcacga catccttgcc gtcctccagc aacgactcga
     4861 cgctgcggcg cgaggtgccg taccagttgc cgtggacgtg cgcatgctcg aagaactggc
     4921 cgtcgtcgac catcgcgcgg aacgtcgtcg tgtcgacgaa gtgatattcg cggccgtcgc
     4981 gctcctggcc gcgcggcgag cgcgtcgtgt gcgagacgga gaccgccagc tgcggctcgt
     5041 gctggagcag ggccttgacg agggtggact tgccggcgcc gctgggcgcg gcgacgacga
     5101 agagcttgcc ggggagttcc atggtgcggg cgccgcgatc gaagccgttc attatcgtcg
     5161 caaacgtcgc tgcccgatcg gggcgcggca cgcctgcggc ggcacaagtt cgcgggatgc
     5221 ggcccggcgc gtcgtcaggc ggcggtgccg tcgaacaccg tggtgaggcc ggggctgcgc
     5281 agcaggctcg ccggcgtcac ggtctcgcgc tgccagggga tgcggccgaa ataccaggcg
     5341 cgccagaagc cgaggtcggc accgggcgac tcgtgcagca gaccggcgaa cgtctcgatg
     5401 cggccgacct ggacgcccgc gccctcctgc agcacctcgc gcacgtagcg cgagcagaac
     5461 tggcgccgcg agtgcaggtc gaatccggtg tcgtagaaca cgtgcgcgcg tt
//
LOCUS       DASVLN010000248         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_334830, whole genome shotgun sequence.
ACCESSION   DASVLN010000248 DASVLN010000000
VERSION     DASVLN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334830"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..894)
                     /locus_tag="VF453_05735"
     CDS             complement(142..894)
                     /locus_tag="VF453_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX7687183.1"
                     /translation="MTSRIEYEPATMSEQDGVRYLHLGTPWVQGAMRVRKPQAIELEY
                     VQRMMAWMLWRPADEVARGHAVQLGLGAAAITRFTHGAMRMRTTAVELNPTVISACRM
                     WFKLPPDDARLEVVQGDAGRWVEDPAHLQSADVLSVDLYDHEAAAPVLDSDAFYAACR
                     GVLAHGGLMAVNLFGRDASFPASAARIASAFGSDQVWSLRPTREGNTVVIAARGVEVP
                     DRDTLAARADNIEARYGLPARKWLRMVRPLTL"
     gene            complement(891..1379)
                     /locus_tag="VF453_05740"
     CDS             complement(891..1379)
                     /locus_tag="VF453_05740"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEX7687184.1"
                     /translation="MLEGLAPVIAPDTRLVVLGSFPGVASLAAQQYYGHPRNAFWRLL
                     SALWGEDLVGRDYAGRLEALRRHRVGLWDVYRACERAGSLDSAIRVPEPNDLARLRTL
                     APGLRAVAHNGAESARSRRLTAALGVDVHALPSTSPANAAWSFERKLAAWRDVFSRYD
                     PQ"
BASE COUNT          197 a          566 c          595 g          191 t
ORIGIN      
        1 aggcggccgc tcggcgccgc gtgccgcagc gcgggcgcgc cggcggacgg gccgggcgcg
       61 ctcgcggtcg ggctcgcgga tcgcgcggcg ggtcgggcgg cgggcggagc gggcgtcgga
      121 gtggaagtcg gcatgcgggc cttagagcgt caacgggcgc accatgcgca gccacttgcg
      181 cgcgggcagg ccgtagcggg cctcgatgtt atccgcgcgg gcggcgagcg tgtcgcgatc
      241 gggcacctcg acgccgcgcg ccgcgatgac gacggtgttg ccctcgcgcg tggggcgcag
      301 gctccagacc tggtcgctgc cgaaggccga cgcgatgcgc gccgcgctgg cgggaaagct
      361 cgcgtcgcgg ccgaacaggt tgaccgccat caggcccccg tgcgcgagca cgccgcggca
      421 ggcggcgtag aacgcgtcgc tgtccaggac gggcgccgcc gcctcgtggt cgtagaggtc
      481 cacgctcagc acgtcggccg actgcaggtg ggccggatcc tcgacccagc gcccggcgtc
      541 gccctgcacg acctccaggc gcgcgtcgtc cggcggcagc ttgaaccaca tccggcaggc
      601 ggagatcacc gtcggattga gctccaccgc ggtcgtgcgc atgcgcatcg cgccgtgggt
      661 gaagcgcgtg atcgcggccg caccgaggcc gagctgcacc gcgtggccgc gcgcgacctc
      721 gtcggccggt cgccacagca tccaggccat catgcgctgc acgtattcga gctcgatcgc
      781 ctgcggcttt cgcacgcgca tcgcgccctg cacccacggc gtgcccaggt gcaggtagcg
      841 cacgccgtcc tgctcggaca tggtggcggg ttcgtactcg attctcgagg tcattgcgga
      901 tcgtagcgcg agaagacgtc gcgccaggcc gcgagcttgc gctcgaagct ccaggcggcg
      961 ttggcggggc tggtcgaggg cagcgcatgg acgtccacgc ccagcgccgc ggtcaggcgg
     1021 cgcgagcgtg ccgactcggc cccgttgtgc gccaccgcgc gcaggcccgg cgcgagcgtg
     1081 cgcaggcgcg cgaggtcgtt gggctcgggc acgcggatcg cgctgtccag gctgccggcg
     1141 cgctcgcaag cgcggtagac atcccacagg ccgacgcgat ggcgccgcag cgcctcgagg
     1201 cggccggcat agtcgcgccc gacgagatcc tcgccccaca gcgccgacag cagccgccag
     1261 aacgcgttgc gcggatggcc gtaatactgc tgcgcggcca gcgaggcgac gccggggaag
     1321 ctcccgagca cgacgagccg cgtgtccggc gcgatgacgg gagccaagcc ttcgagcatc
     1381 gcgcgatcct aacccgcgcg ccctcgcccc gcgggccgcg caggcgtcgc cggccgggca
     1441 tccgggccgg cggacgcgcg cccgccgtca ctcacccgtg gccttgtgca cgccgtggtc
     1501 gatcgcctcg ccggtccggt gcgcagcgtg gcggatagca tggccggtc
//
LOCUS       DASVLN010000249         1764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_334854, whole genome shotgun sequence.
ACCESSION   DASVLN010000249 DASVLN010000000
VERSION     DASVLN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334854"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VF453_05745"
     CDS             <1..687
                     /locus_tag="VF453_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7687185.1"
                     /translation="AHVEQAEQAAGRERERQLQARLAELGALAATVAHDIRNPLNIIA
                     MAVAGAPTDTRREVADQVARIANLSRDLLDYAKPWKLATARVDLAAQVRAAAARLPEV
                     TLGDGLAQALPVDADPRRIDQALANLLENARAAAEDAGVGGARARVHVDAETTPQGLQ
                     LHVCDDGPGVPDEIRARLFEPFASRSPGGTGLGLAIVARVMAAHGGSVALTERPPWRT
                     CFTLTFPTPT"
     gene            684..>1764
                     /locus_tag="VF453_05750"
     CDS             684..>1764
                     /locus_tag="VF453_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7687186.1"
                     /translation="MSDTTADIGADTHGRILLVDDEPAFQRLGAAFLRDQGYEVAVAG
                     DGDQALEACSRRRPDVVLLDLAMPPHMDPEIGLELIAHLPPVPVVVLTGHGEHELALR
                     AAELGAWDFLAKPVDPDMLRFVVARALRKARVDAELAALRAANAADDLGLVGQAPAMA
                     QLRAMVRRVAQTGVSVLVLGPTGTGKELVARALHAGGVRSAGPFVAIHCGALSAELLE
                     SELFGHVKGSFTGAYRDQPGLVEAAHGGTLFLDEVGEMPPPMQVKLLRFLQEGTFVAV
                     GGRVQKRADVRVVAATHRDLEAMVREGAFREDLFYRLKGVVLRTPALADRAADVPLLA
                     ARFLRRVAPRAGLSASALGWLAAREW"
BASE COUNT          221 a          663 c          652 g          228 t
ORIGIN      
        1 gcgcacgtcg agcaggccga gcaggccgcc ggccgcgagc gcgagcgcca gctgcaggcc
       61 cgcctggcgg agctcggcgc gctggccgcg acggtcgcgc acgacatccg aaacccgctc
      121 aacatcatcg cgatggccgt agccggcgcg cccacggaca ccaggcgcga ggtcgccgac
      181 caggtcgcgc ggatcgccaa cctgtccagg gatctgctgg actacgccaa gccatggaag
      241 ctcgcgacgg cgcgcgtcga cctcgcggcc caggtgcgcg ccgcggccgc acggctgccc
      301 gaggtgacgc tgggcgatgg tcttgcgcag gcgctgccgg tcgacgccga tccccggcgc
      361 atcgaccagg cccttgcgaa cctgctcgag aacgcccgcg cggccgccga ggatgcgggc
      421 gtgggtggag cacgcgcccg cgtgcacgtc gacgccgaaa ccacgccgca ggggctgcag
      481 ctgcatgtct gcgacgacgg ccccggcgta cccgacgaga tccgcgcccg cctgttcgag
      541 cccttcgcct cgcgcagccc cggcggcacc gggctcggcc tggccatcgt cgcgcgggtg
      601 atggctgcgc acggcggctc ggtcgccctg accgagcgcc cgccctggcg cacctgcttc
      661 accctcacct tcccgacccc gacatgagcg acacgaccgc cgacatcggc gcggataccc
      721 acggccgtat cttgctcgtc gacgacgagc cggcgttcca gcgcctgggc gccgcattcc
      781 tgcgcgacca gggctacgag gtcgccgtcg cgggggacgg cgatcaggcg ctggaggcct
      841 gctcgcgccg gcggccggac gtcgtgctgc ttgatctcgc gatgccgccc cacatggatc
      901 cagagatcgg cctggagctg atcgcgcacc tgccgccggt gcccgtcgtc gtgctgacgg
      961 gccacggcga gcacgagctc gcgctgcgcg ccgcggagct cggcgcctgg gatttcctcg
     1021 ccaagccggt cgaccccgac atgctgcgct tcgtcgtcgc gcgcgcgctg cgcaaggcgc
     1081 gcgtggacgc ggagctcgcc gcgctgcgcg ccgccaatgc cgctgacgac ctcggcctcg
     1141 tcggccaggc accggcgatg gcgcagctgc gcgcgatggt gcgtcgggtc gcgcagaccg
     1201 gcgtgagcgt gctcgtgctc gggccgaccg gcaccggcaa ggagctcgtc gcgcgcgccc
     1261 tgcacgcagg aggcgtgcgc agcgcggggc cgttcgtcgc catccactgt ggcgcgctgt
     1321 cggccgagct gctcgagagc gagctgttcg gtcacgtcaa gggcagcttt accggcgcgt
     1381 accgggacca gcccgggctc gtggaggccg cgcacggcgg cacgctgttc ctcgacgagg
     1441 tcggcgagat gccgccgccg atgcaggtca agctgctgcg tttcctgcag gaaggcacct
     1501 tcgtcgcggt cgggggacga gtgcagaaac gcgccgacgt gcgcgtggtc gcggcgacgc
     1561 accgcgacct cgaagcgatg gtgcgcgagg gcgcgttccg cgaagacctc ttctaccgcc
     1621 tcaagggcgt cgtgctgcgc acgccggcgc tcgcggaccg cgccgccgac gtgccgctgc
     1681 tggccgcgcg cttcctgcgc cgtgtcgcac cgagggcggg gctgtcggcg agcgcgctcg
     1741 ggtggctcgc cgcgcgcgag tggc
//
LOCUS       DASVLN010000250         1717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_66141, whole genome shotgun sequence.
ACCESSION   DASVLN010000250 DASVLN010000000
VERSION     DASVLN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66141"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VF453_05755"
     CDS             complement(<1..628)
                     /locus_tag="VF453_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7687187.1"
                     /translation="MTPADDRPIDGDAAAPPLRVLHADADLVVIDKPAGLLVHRSSLD
                     EHETRSAAELLRAQRGEPLWLLHRLDKATSGLLAFARNVDTARALGPAFESGRVGKRY
                     LALVRGWPQDDGVIDYPLARDPELPSAGQPRLPATTRWHVQRRFEWPFPDGRHATSRY
                     ALVEVEPLSGRRHQIRRHFKHLAHPLVGDTTHGKGAHNRAVAAWLGASR"
     gene            complement(686..>1717)
                     /locus_tag="VF453_05760"
     CDS             complement(686..>1717)
                     /locus_tag="VF453_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7687188.1"
                     /translation="IAEADAQAARACWATIALAAISIVTGAACAWLITRSVTAPLDAA
                     VRAVRTVADGDLGGRIVVQGRDELASLLGSLQAMQSSLRKLVGEVRGTTDSIQVASRD
                     VAQGSQDLSARTERAAASLQETASTMEQISSTLRHTADAAGTAEELARGATQAAEAGG
                     AVVDRMTATMERITSYAARIGEIVGIIDGIAFQTNILALNAAVEAARAGEQGRGFAVV
                     AGEVRSLAQRSAQAAGEIGKLIGESGQAVGDGGGLVAETRTSMAAIADSIRQVGAVIA
                     EIRTASSEQADAVAQVNTAVSDLDDATQQNAALVEQTAAAADSLREQAHRLADAVSVF
                     RVGDDAGGA"
BASE COUNT          190 a          642 c          652 g          233 t
ORIGIN      
        1 gccgcgatgc ccccagccac gcggcgaccg cccggttgtg cgcgcccttg ccgtgcgtcg
       61 tgtcgcccac cagcggatgc gcgagatgct tgaagtggcg ccggatctgg tggcgtcggc
      121 cgctgagcgg ctcgacctcg accagggcgt agcggctcgt cgcgtgacgg ccgtcgggga
      181 acggccattc gaagcgccgc tgcacgtgcc agcgcgtcgt cgcgggcagg cgcggctggc
      241 cggcggaggg cagctcgggg tcgcgcgcga gcgggtagtc gatcacgccg tcgtcctgcg
      301 gccagccgcg caccagcgcg agatagcgct tgcccacgcg cccggactcg aacgccggcc
      361 cgagcgcgcg tgccgtgtcg acgttgcgcg cgaacgcgag caggcccgac gtcgccttgt
      421 ccagccggtg cagcagccac agcggctcgc cgcgctgtgc gcgcagcagc tccgcggcgc
      481 tgcgggtctc gtgctcgtcg agcgacgagc gatgcaccag caggccggcg ggcttgtcga
      541 tgacgacgag gtcggcgtcg gcgtggagca cgcgcagcgg cggcgcggcg gcgtcgccgt
      601 cgatggggcg gtcgtcggcc ggggtcatgt cggttcagtt cgcggcatcg tcggcggatt
      661 gtccgacgcc aagcggtgcg cgcgttcagg cgcccccggc gtcgtcaccc acccggaaga
      721 ccgacaccgc gtccgcgagc cggtgcgcct gctcgcgcag gctgtcggcg gcggcggcgg
      781 tctgctccac gagcgcggca ttctgctgcg tggcgtcgtc gaggtcggag accgcggtgt
      841 tgacctgcgc cacggcgtcg gcctgctcgg acgaggcggt gcggatctcc gcgatcaccg
      901 cgccgacctg gcggatcgag tcggcgatcg ccgccatcga cgtccgcgtc tcggcgacca
      961 gcccgccgcc gtcgcccacc gcctggcccg actcgccgat cagcttgccg atctcgccgg
     1021 ccgcctgcgc ggagcgctgc gcgaggctgc gcacctcgcc ggcgaccacc gcgaagccgc
     1081 gcccctgctc gccggcgcgc gcggcctcga ccgcggcgtt gagcgcgagg atgttggtct
     1141 ggaaggcgat gccgtcgatg atgcccacga tctcgccgat gcgcgccgcg tagctcgtga
     1201 tgcgctccat cgtcgcggtc atgcggtcga cgaccgcgcc gccggcctcg gccgcctgcg
     1261 tcgcgccgcg cgccagttcc tcggcggtgc ccgcggcgtc ggcggtgtgg cgaagcgtgc
     1321 tcgagatctg ctccatcgtc gatgccgtct cctgcagcga cgcggcggcg cgctcggtgc
     1381 gggcggacag gtcctggctg ccctgcgcga cgtcgcggct ggcgacctgg atcgaatccg
     1441 tcgtgccgcg cacctcgccc accagcttgc gcagggagga ctgcatcgcc tgcaggctcc
     1501 cgagcagcga ggcgagttcg tcgcggccct ggacgacgat gcgcccgccc aggtcgccgt
     1561 ccgcgaccgt ccgcacggcg cgcaccgcgg cgtcgagcgg cgccgtcacc gagcgcgtga
     1621 tgagccacgc gcaggccgcg ccggtgacga tggagatcgc cgccagcgcg atcgtcgccc
     1681 agcacgcgcg cgcggcctgg gcgtcggcct cggcgat
//
LOCUS       DASVLN010000251        10627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_281929, whole genome shotgun sequence.
ACCESSION   DASVLN010000251 DASVLN010000000
VERSION     DASVLN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10627
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281929"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..2971
                     /locus_tag="VF453_05765"
     CDS             359..2971
                     /locus_tag="VF453_05765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7687189.1"
                     /translation="MTNTNADSARWQRVKDLLARALELPPAEQRGFLERECGDDAGLR
                     TEIESLLSAAHDADDRLHSLAPDWIADAVHAAGGGSWIGRRVGAWRIVALIGGGGMGE
                     VYRAERVDGQFAQDVAVKVMRGGFNPEGLAARFKAERQILASLDHPNLAKVLDGGISE
                     DGLPWFVMELVDGEPIDVYVAREKRSIVERVRLFRSVCQVVHYAHQKRVVHRDLKADN
                     ILVRRDGVVKLVDFGIAKRIDPAAAEPPTRTATAQRAMTLVYSSPEQVRGGEITPASD
                     IYSLGVVLYRLLTDTSPYPPATTENPYELTRAICDTEPAPPSAAATTRSEADRRRLRG
                     DLDAVVMMALRKDPARRYASAEAMSEDLFRHLEGLPVQARRGAWSYRAGRFLLRNRAL
                     VGAVMAANLALVAGIAVAGYEAWQANQQRARAERDFARLRQLAGVFVFDIHDAIQTLP
                     GSTAARRLVAQTALSYLQDLSAESAGDGMLQTEVASGYRKVGDILGRPYVANLGDPKG
                     AHENYERARRMLQPLVAAPLASAQGRAARRELVQLLQHEGSLYDSLGDDKQAEATIAA
                     ALPMAQALADLPDAAPSDRLALASSYVRLSAVQFYQGHPQDYLKSSDRSLALLQDVLA
                     RTPDDQEALQQQSVARDLRGQYLTQDDTPAGRQAAVAAFEQSVHALDHLHALAPQRAD
                     VTRRLAGSLSNVGSARAFAGDLKGSAVALRRAIALLGELVARDPLDMDARGTRALVTA
                     NLVDLLRETGDYDEAIRDGNDALAMFDQMAAGARQDASIRSAMGLAHFVLAKSFVARA
                     HANPKRPAFAAGDRNEACRHFDTSLDILQALKGSAGIAPNNLDPETVRKEMQGCPAHE
                     PAVAATGAAASATR"
     gene            complement(2984..3601)
                     /locus_tag="VF453_05770"
     CDS             complement(2984..3601)
                     /locus_tag="VF453_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7687190.1"
                     /translation="MDHLEPFRNDELTVTISELLVATADSADPLLDDSVAELLHAMRL
                     RMEMDVVFVSEFIDGRRMFRHVDAAPGALDIRAGDSNPIEESVCQRVVEGRVPELVHD
                     LSALPADQLPQLPARIGAHLSTPIVLKDRSVYGTLCCFSAAPRPELVQADLDKLRLCA
                     VLVARKIELGQARGLQEPPPDWQLEPKARYESPTWQRASSRGDSR"
     gene            3883..3975
                     /gene="kdpF"
                     /locus_tag="VF453_05775"
     CDS             3883..3975
                     /gene="kdpF"
                     /locus_tag="VF453_05775"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEX7687191.1"
                     /translation="MSWMVVLGGVVSVGLLAYLVAALLQPEKFS"
     gene            3972..5762
                     /gene="kdpA"
                     /locus_tag="VF453_05780"
     CDS             3972..5762
                     /gene="kdpA"
                     /locus_tag="VF453_05780"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX7687192.1"
                     /translation="MTGHAWSVLAAYLVVLIALAIPAGRWIAHVMEGRVAFAGRVESL
                     FYRLAGVKADEEMGWLQYALAILLFNGLGVVAVYLLQRLQQWLPLNPQAMANVSPDSS
                     FNTALSFVTNTNWQGYVGETTMSYLTQMLGLAVQNFFSAATGIVVAVALIRGLARHTA
                     KGIGNAWVDLTRCTLWILLPISFVYALFLVQQGSIQNFDAYKDVHTLEVVKWQEPRTG
                     ADGQPVKDAKGNPVMVDKSSDHQTLAMGPVASQESIKMLGTNGGGFFNANSAHPYENP
                     TPLSDFVQMVSIFLIPAALCLAFGRMVGDRRQGWAVMAAMALLFVAGAIGAMHFEQQG
                     NPQLAALHVDQSTGALQAGGNMEGKETRFGIAESALFATITTDASCGAVNAMHDSFTP
                     LGGMVPLVNMQLGEVVFGGVGTGLYGMLVFAIIAVFIAGLMIGRTPEYLGKKIEAYEM
                     KMSSIAILVTPLLVLAGTAIAAMADAGRAGTSNPGAHGFSEILYAFTSAANNNGSAFA
                     GLSANTPFYNVMLGIAMWFGRFGVIVPVLALAGSLAAKKRLATTGGTMPTHGPLFVAL
                     LVGTVLLVGLLNYVPALALGPAVEHLTLWK"
     gene            5767..7896
                     /gene="kdpB"
                     /locus_tag="VF453_05785"
     CDS             5767..7896
                     /gene="kdpB"
                     /locus_tag="VF453_05785"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX7687193.1"
                     /translation="MTRKTFTLFDPALAKPAMVDALRKLSPRVQWRNPVMFVVYVGSI
                     LTTALWVQALVGKGEAPAGFILAIALWLWFTVLFANFAEALAEGRSKAQAASLRSLKR
                     QTWAKKLEGRLEAPLHDAKWIPCESANLRKGDHCYVIAGDVIPADGEVVEGVASVDES
                     AITGESAPVIRESGGDFSAVTGGTKVLSDWIVMRVTVNPGETFVDRMIAMVESAKRQK
                     TPNEVALTILLVALTIVFLAVTVTLLPFSLYAVDANKAGSPITVTVLISLLVCLIPTT
                     IAGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASVFLP
                     APGVKESDLADAAQLASLADETPEGRSIVVLAKQKYNLRERDLQAMGASFVHFSAQTR
                     MSGVDLPMVVESAQAVGGDSAGGPLRQVRKGAADAIRRHVQALGAAFPAALEATVEAV
                     ARRGSTPLVVSDGTRVLGVIELKDVVKGGIKERFAELRRMGIKTVMVTGDNRLTAAAI
                     AAEAGVDDFLAEATPEQKLELIRSHQAEGRLVAMTGDGTNDAPALAQADVAVAMNSGT
                     QAAKEAGNMVDLDSNPTKLIEVVETGKQMLMTRGALTTFSIANDVAKYFAIIPAAFVT
                     TYPQLRALDVMRLASPSSAILSAVIFNALIIVFLIPLALKGVQYRPLGASVLLRKNLL
                     VYGLGGVIVPFIGIKAIDLVLAACGLV"
     gene            7907..8485
                     /gene="kdpC"
                     /locus_tag="VF453_05790"
     CDS             7907..8485
                     /gene="kdpC"
                     /locus_tag="VF453_05790"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX7687194.1"
                     /translation="MKSLVRPAVTLFVLLTAVTGVVYPLAVTGVAKAAFPGQASGSLV
                     MKDGQAVGSALIGQNFSDPKYFWGRPSATSPMAYNGANSGGSNQGPTNPALVDAVKGR
                     IDALEAADPGNAAPVPVDLVTASASGLDPEISVAAARYQAARVARVRGLSPAVVGGLI
                     DRHATDRLLGLLGEPRVNVLELNLDLDRTATH"
     gene            8598..9467
                     /locus_tag="VF453_05795"
     CDS             8598..9467
                     /locus_tag="VF453_05795"
                     /inference="COORDINATES: protein motif:HMM:NF021230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEX7687195.1"
                     /translation="MPRHLLFAALAATPLLNPIAAHAQAAAAPGAAASAPAPSLTATA
                     NVNVVSDYRFRGIDQTWGRPAVQGGADLSWANGLYAGAWLSNVSGNVYPGGSLEVDLY
                     AGYNGKINDDFGYTVGGYGYVYPGANIDKSACPSAAYPTAPCTPPSQSPNTFELNAGA
                     SWKWISYKLSVSMTDYFGANAGTGYSGRTRGTMYHDITVTYPIMDDLSLAGHVGRTDV
                     KGSYAGANPDYTDWRLTLTKTFTGGWSAAVAGAGANNDRFYRPPVGGLSYANAGTRDL
                     NKPTLIVQLGRAF"
     gene            9479..>10627
                     /locus_tag="VF453_05800"
     CDS             9479..>10627
                     /locus_tag="VF453_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401404.1"
                     /note="sensory histidine kinase in two-component
                     regulatory system with KdpE; signal sensing protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HEX7687196.1"
                     /translation="MTDSRPDPDALLAQLKAEEAQARRGRLKIFFGATAGVGKTYAML
                     SAARAQRAQDVDLVVGVVETHGRLETQSLLDGLEVVPLRDVSYRGHALKEFDLDAVLT
                     RKPSLVLVDELAHSNVQGSRHPKRWQDVQELLAAGIDVWTTVNVQHLETLNDVVNGIT
                     GVRVWETVPDRVFDEADEVVIVDLPPDDLLQRLRDGKVYMPQQAATAVRNFFRKGNLI
                     ALRELALRRTADRVDDDMLHWRRARAVAPVWQTREAMLVCIGPDERADKLVRTGARLA
                     AQLDVPWHCVYVETPRLQRLPPARRERVLRLLRVAQESGAETAVLAGSQLAAAIVRYA
                     HEHNLPRVVLGRDVRQVARRWRTSLADAVGTMGADLDVLQVALPRANGD"
BASE COUNT         1553 a         3841 c         3717 g         1516 t
ORIGIN      
        1 ggcctgctgg gcggccgtgg tgtcgaacag gaatgggctg aacgaattcg gaccaagcga
       61 ggggacggcc atcgaaagct cctgacgtgg gcattgctcg acgacggcag ccggagttcc
      121 gtgccggggg acgagagcgc cggtggacgc cccgtgtcga gctccggagc agtctaggac
      181 ggccgccgcg cagggcggaa atgccaaaag acggacgttg tatgccgaaa cgacatgggg
      241 tttccaccta gtcccggccg tccgggttgc aattcgatga catgttccgg cgccccgtcg
      301 aggtaacctc gcgccgctgc acccgtggcc gggccgcggc gacgccaagg agcccgaggt
      361 gaccaacacg aacgccgatt ccgcgcgctg gcagcgcgtg aaggacctgc tcgcgcgggc
      421 gctggagctg ccgccggccg agcagcgcgg cttcctcgag cgcgagtgcg gcgacgacgc
      481 cggcctgcgc acggagatcg agtcgctgct gtccgcggcc cacgacgccg acgaccgcct
      541 gcattcgctc gcccccgact ggatcgccga cgccgtgcat gcggcgggcg gcggctcgtg
      601 gatcggccgc cgcgtgggcg cctggcgcat cgtcgcgctg atcggcggcg gcggcatggg
      661 cgaggtctac cgcgccgagc gcgtcgacgg ccagttcgcg caggacgtgg cggtcaaggt
      721 gatgcgcggc ggcttcaatc ccgagggact ggccgcgcgc ttcaaggcgg agcgccagat
      781 cctcgcgagc ctcgaccatc ccaacctcgc caaggtcctc gacggcggca tcagcgagga
      841 cggcctgccc tggttcgtga tggagctggt cgacggcgag cccatcgatg tctacgtcgc
      901 gcgcgagaag cggtccatcg tcgagcgcgt gcggctgttc cgcagcgtct gccaggtggt
      961 gcactacgcg caccagaagc gcgtcgtgca ccgcgacctc aaggccgaca acatcctcgt
     1021 gcgccgcgac ggcgtcgtga agctggtgga cttcggcatc gccaagcgca tcgatcccgc
     1081 cgcggccgag ccgccgacgc gcaccgccac cgcgcagcgc gcgatgacgc tcgtctactc
     1141 gagcccggag caggtgcgcg gcggcgagat cacgccggcc agcgacatct actccctggg
     1201 cgtcgtgctg taccgcctgt tgaccgacac gagcccgtat ccgcccgcga cgaccgagaa
     1261 cccgtacgag ctcacgcgcg ccatctgcga caccgagccg gcgccgccga gcgccgccgc
     1321 gacgacgcgc tcggaggccg accgccggcg cctgcggggc gacctggacg cggtcgtgat
     1381 gatggcgctg cgcaaggatc ccgcgcggcg ctacgcctcg gccgaggcga tgtcggaaga
     1441 cctgttccgc catctcgaag gcctgcccgt gcaggcacgc cgcggcgcgt ggagctaccg
     1501 cgccggccgc ttcctgctgc gcaaccgggc gctggtgggc gcggtcatgg cggccaacct
     1561 ggcgctggtg gcgggcatcg ccgtcgcggg ctacgaggcg tggcaggcca accagcagcg
     1621 cgcccgggcc gagcgcgact tcgcccgcct gcgccagctc gccggcgtgt tcgtgttcga
     1681 catccacgac gcgatccaga cgctgcccgg ctccaccgcc gcacgccggc tcgtcgccca
     1741 gaccgcgctc agctacctgc aggacctgag cgcggagtcc gcgggcgacg gcatgctgca
     1801 gaccgaggtc gcgtccggct atcgcaaggt cggcgacatc ctgggccgtc cctacgtcgc
     1861 caacctgggc gatcccaagg gcgcccacga gaactacgag cgcgcgcgcc gcatgctgca
     1921 gccgctcgtc gcggcgccgc tcgcgagcgc gcagggacgc gcggcgcggc gcgagctggt
     1981 gcagctgctg cagcacgagg gctcgctgta cgactccctc ggcgacgaca agcaggccga
     2041 ggcgaccatc gccgcggcat tgccgatggc ccaggcgctc gccgacctgc cggacgcggc
     2101 gccatccgac cgcctggcgc tggcgtccag ctacgtccgg ctgagcgcgg tgcagttcta
     2161 ccagggccat ccgcaggact acctgaagag ctccgaccgc tcgctcgcgc tgctgcagga
     2221 cgtgctcgcc cgcacgcccg acgaccagga ggcgctgcag cagcagtcgg tcgcgcgcga
     2281 cctgcgcggg cagtacctca cgcaggacga cacgccggcc ggccgccagg ccgccgtggc
     2341 ggcgttcgag cagtcggtgc acgcgctgga ccacctgcac gcgctcgcgc cgcagcgcgc
     2401 cgacgtcacg cgccggctcg cgggctcgct cagcaacgtc ggctcggcgc gcgccttcgc
     2461 gggcgacctg aagggctcgg ccgtggcgct gcggcgcgcc atcgcgctgc tgggcgagct
     2521 ggtcgcgcgc gacccgctcg acatggacgc gcgcggcacg cgggcgctcg tcacggccaa
     2581 cctggtcgac ctgctgcgcg agaccggcga ctacgacgag gcgatccgcg acggcaacga
     2641 cgcgctcgcg atgttcgacc agatggccgc gggcgcacgc caggacgcct cgatccgcag
     2701 cgcgatgggg ctcgcccatt tcgtgctcgc caagtccttc gtcgcgcgcg cgcacgccaa
     2761 cccgaagcgt cccgcgttcg ccgccggcga ccgcaacgag gcgtgccgcc acttcgacac
     2821 ctcgctcgac atcctgcagg cgctcaaggg cagcgcgggc atcgcgccga acaacctcga
     2881 cccggagacg gtgcgcaagg agatgcaggg ctgccccgcg cacgagcccg cggtcgcggc
     2941 gacgggggct gcggcgtccg cgacgcgttg acggccgatc cgatcagcgc gagtcgccgc
     3001 gcgacgaggc gcgctgccag gtcggcgact cgtagcgtgc cttcggctcc agctgccagt
     3061 cgggcggcgg ctcctgcagg ccgcgcgcct gaccgagctc gatcttgcgc gccaccagca
     3121 cggcgcacag gcgcagcttg tcgaggtcgg cctgcacgag ctccggccgc ggcgccgcgc
     3181 tgaagcagca cagggtgccg tagacgctgc ggtccttcag cacgatgggc gtgctcaggt
     3241 gcgcgccgat gcgcgccggc agctgtggca attggtcggc gggcagcgcc gacaggtcgt
     3301 gcaccagctc cggcacccgg ccctccacca cgcgctggca gaccgattcc tcgatcgggt
     3361 tcgagtcccc cgcgcggatg tcgagcgcgc cgggcgcggc gtcgacgtgg cggaacatcc
     3421 gccgcccgtc gatgaactcg gagacgaaga cgacgtccat ctccatgcgc agccgcatcg
     3481 cgtgcagcag ctccgcgacc gagtcgtcga gcagcgggtc ggcggagtcc gcggtggcga
     3541 cgagcagctc cgagatggtg acggtgagtt cgtcgttcct gaacggttcc agatggtcca
     3601 tgtgcgatcg cttccggggc cgcctgctcc ctcgacgcac gcggcccgcc gacgcagtgt
     3661 actgaaagcg gtgtcggccg cggaaagccg cgggcggctc ctgacgccgt ctttatgtcg
     3721 gcggccccaa tctttgcgac atctttatgc cgcccttcct acgatgactt ctcatctaca
     3781 ggaggacgac atggacgtcg tttatgcggt gaccgccctc ggattctggc tgctgatggc
     3841 cgggatggcg gtggggtgcg caaagcttgg agggccgaag ccatgagctg gatggtcgtg
     3901 cttggcggcg tggtgtcggt gggcctgctg gcctacctcg tggcggcgct gctgcagccg
     3961 gagaagttct catgaccgga cacgcctgga gcgtgctcgc ggcgtacctg gtcgtcctga
     4021 tcgcgctcgc gatcccggcc ggccgctgga tcgcgcacgt catggaagga cgcgtcgcct
     4081 tcgcgggccg cgtcgaatcg ctcttctacc gcctggccgg cgtgaaggcc gacgaggaga
     4141 tgggctggct gcagtacgcg ctggcgatcc tgctgttcaa cggcctgggc gtcgtcgccg
     4201 tctacctgct gcagcgcctg cagcagtggc tgccgctgaa cccgcaggcg atggccaacg
     4261 tgtcgccgga ctcgtcgttc aacacggcgc tgagcttcgt caccaacacg aactggcagg
     4321 gctacgtcgg cgagacgacg atgagctacc tcacccagat gctcgggctg gcggtgcaga
     4381 acttcttctc cgccgcgacc ggcatcgtgg tggcggtcgc gctgatccgc ggcctcgcca
     4441 ggcataccgc aaagggtatc ggcaacgcct gggtcgacct cacgcgctgc acgctgtgga
     4501 tcctgctgcc gatctcgttc gtctacgcgc tgttcctggt gcagcaggga tcgatccaga
     4561 acttcgacgc ctacaaggac gtgcacacgc tcgaggtcgt caagtggcag gagcccagga
     4621 cgggcgccga cggccagccg gtcaaggacg ccaagggcaa tccggtgatg gtggacaagt
     4681 cgtccgacca ccagaccctc gcgatggggc cggtggcgtc gcaggagtcg atcaagatgc
     4741 tgggcaccaa cggcggcggc ttcttcaacg ccaactcggc gcatccctac gagaacccga
     4801 cgccgctgtc cgacttcgtg cagatggtct cgatcttcct gatccccgcg gcgctgtgcc
     4861 tggcgttcgg gcggatggtc ggcgaccggc gccagggctg ggcggtgatg gcggcgatgg
     4921 cgctgctgtt cgtcgccggc gcgatcggcg cgatgcactt cgagcagcag ggcaacccgc
     4981 aactggcggc gctgcacgtg gaccagtcga ccggcgcgct gcaggccggc ggcaacatgg
     5041 agggcaagga gacgcgcttc gggatcgccg agtcggccct gttcgccacc atcacgacgg
     5101 acgcgtcctg cggcgccgtc aacgcgatgc acgactcgtt cacgccgctg ggcggcatgg
     5161 tgccgctggt gaacatgcag ctgggcgagg tggtcttcgg cggcgtcggc acgggcctgt
     5221 acggcatgct ggtgttcgcg atcatcgcgg tcttcatcgc gggcctgatg atcggccgca
     5281 cgcccgagta cctcggcaag aagatcgagg cctacgagat gaagatgagc tcgatcgcga
     5341 tcctggtcac gccgctgctc gtgctcgcgg gtaccgcgat cgccgcgatg gccgatgccg
     5401 ggcgcgcggg cacgtccaat cccggcgcgc acggcttctc ggagatcctg tacgccttca
     5461 cctcggcggc caacaacaac ggcagcgcgt tcgcgggcct gtccgccaac acgcccttct
     5521 acaacgtgat gctgggcatc gcgatgtggt tcggccgctt cggcgtgatc gtgccggtgc
     5581 tggcgctcgc gggctcgctc gctgcgaaga agcgcctcgc cacgaccggc gggacgatgc
     5641 ccacgcacgg cccgctgttc gtcgcgctgc tggtcggcac ggtgctgctg gtcggcctgc
     5701 tgaactacgt gccggcgctc gcgctgggcc cggccgtcga gcacctgacg ctctggaagt
     5761 gaacccatga cacgcaagac tttcacgttg ttcgacccgg cgctggccaa gccggcgatg
     5821 gtggacgcgc tccgcaagct cagcccgcgc gtccagtggc gcaacccggt gatgttcgtc
     5881 gtctacgtcg gcagcatcct caccaccgcg ctgtgggtgc aggccctggt cggcaagggc
     5941 gaggcgccgg ccggcttcat cctcgcgatc gcgctgtggc tgtggttcac cgtgctgttc
     6001 gccaatttcg ccgaggcgct cgccgagggc cgcagcaagg cgcaggccgc gtcgctgcgc
     6061 agcctcaaga ggcagacctg ggcgaagaag ctcgaagggc ggctcgaggc accgctccac
     6121 gacgcgaagt ggatcccgtg cgaatccgcg aacctgcgca agggcgacca ttgctacgtc
     6181 atcgccgggg acgtcatccc ggccgacggc gaagtggtcg agggcgtcgc ctcggtggac
     6241 gagagcgcca tcaccggcga gtccgcgccg gtcatccgcg agtcgggcgg cgacttctcg
     6301 gccgtcaccg gcggcaccaa ggtgctgtcc gactggatcg tgatgcgcgt gaccgtgaac
     6361 cccggcgaga ccttcgtcga ccgcatgatc gcgatggtcg agagcgccaa gcgccagaag
     6421 acgcccaacg aggtcgcgct gaccatcctg ctcgtggcgc tgaccatcgt gttcctcgcc
     6481 gtgaccgtga cgctgctgcc gttctcgctg tacgccgtcg acgccaacaa ggccggctcg
     6541 ccgatcacgg tgacggtgct gatctcgctg ctcgtgtgcc tgatcccgac gacgatcgcc
     6601 ggcctgctgt cggccatcgg cgtggccggc atgagccgca tgatgcaggc caacgtgatc
     6661 gcgacctcgg gccgtgcggt cgaggctgcc ggcgacgtcg acgtgctcct gctggacaag
     6721 accggcacga tcacgctggg caaccggcag gcgtcggtct tcctgccggc gccgggcgtc
     6781 aaggaatcgg acctcgcgga cgccgcgcag ctcgcatcgc tggccgacga gacccccgag
     6841 ggccgcagca tcgtcgtgct ggccaagcag aagtacaacc tgcgcgagcg cgacctgcag
     6901 gcgatgggtg cgagcttcgt ccacttctcg gcgcagacgc gcatgagcgg ggtcgacctg
     6961 cccatggtcg tcgagtcggc gcaggcggtc ggcggcgaca gcgcgggcgg ccccctgcgc
     7021 caggtgcgca agggcgccgc cgacgcgatc cgccgccatg tgcaggcgct gggcgcggcc
     7081 ttcccggcgg cgctggaggc gacggtcgag gcggtcgccc gccgcggcag cacgccgctc
     7141 gtcgtgagcg atggcacccg ggtgctgggc gtgatcgagc tgaaggacgt cgtcaagggc
     7201 ggcatcaagg agcgcttcgc cgagctgcgc cgcatgggca tcaagaccgt gatggtgacc
     7261 ggcgacaacc gcctgaccgc cgccgcgatc gccgccgagg ccggcgtcga cgacttcctc
     7321 gccgaggcga caccggagca gaagctcgag ctgatccgct cgcaccaggc cgaaggccgg
     7381 ctcgtcgcga tgaccggcga cggcaccaac gacgcacccg cgctcgcgca ggccgacgtc
     7441 gcggtggcga tgaactcggg cacgcaggcc gccaaggagg ccggcaacat ggtcgacctc
     7501 gactccaatc cgaccaagct gatcgaggtc gtcgagaccg gcaagcagat gctcatgacg
     7561 cgcggcgcgc tgaccacctt cagcatcgcc aacgacgtcg ccaagtactt cgcgatcatc
     7621 ccggccgcct tcgtcacgac ctatccgcag ctgcgcgcgc tcgacgtgat gcggctcgcg
     7681 agcccgtcgt cggccatcct gtcggcggtg atcttcaacg cgctgatcat cgtcttcctc
     7741 atcccgctgg cgctcaaggg cgtccagtac cggccgctcg gcgcctccgt gctgctgcgc
     7801 aagaacctgc tggtctacgg cctcggcggc gtgatcgtgc ccttcatcgg catcaaggcg
     7861 atcgacctcg tgctggccgc ctgcggcctc gtctgaaagc atccccatga aatcgctcgt
     7921 ccgccccgcg gtgacgctgt tcgtcctgct caccgccgtc accggcgtcg tctatccgct
     7981 ggccgtcacg ggcgtcgcga aggcggcgtt ccccgggcag gcctccggca gcctcgtcat
     8041 gaaggacggc caagccgtcg gctccgcgct gatcggccag aacttcagcg acccgaagta
     8101 cttctggggg cgcccgtcgg ccacgagccc gatggcctac aacggcgcca actcgggcgg
     8161 ctcgaaccag gggccgacga acccggcgct cgtcgacgcc gtcaagggcc gcatcgacgc
     8221 gctcgaggca gccgacccgg gcaacgccgc gccggtgccg gtcgacctcg tcacggcatc
     8281 ggccagcggc ctcgatcccg agatcagcgt ggccgccgcg cgctaccagg ccgcccgcgt
     8341 cgcacgcgtg cgcgggctgt cgccggcggt cgtcggcggc ctgatcgacc gtcacgccac
     8401 cgaccgcctg ctcggcctgc tcggcgagcc gcgcgtcaac gtgctggaac tcaacctcga
     8461 cctcgatcgc accgcgacgc attgagcgcc gtacggcgcg cacgcgattc cctcggccct
     8521 tcgtccggcc ttcctggtcc gtccgcggcc cgcgccgcgc gccgcgggcg gccaggcgcc
     8581 tttttcgcca tcccatcatg ccccgtcatc tcctgttcgc cgcgctcgcg gccacgccgc
     8641 tcctgaaccc gatcgccgcg cacgcgcagg ccgcggccgc gcccggcgcg gcggcttccg
     8701 cacccgcgcc gtcgctgacc gcgaccgcca acgtcaacgt cgtctccgac taccgcttcc
     8761 gcggcatcga ccagacctgg ggccgtcccg ccgtgcaggg cggcgccgac ctgtcctggg
     8821 cgaacggcct gtacgccggc gcctggctgt ccaacgtgtc gggcaacgtc tatccgggcg
     8881 gcagcctgga ggtcgacctc tacgccggct acaacggcaa gatcaacgac gacttcggct
     8941 acaccgtggg tggatacggg tacgtgtacc ccggcgcgaa catcgacaag tcggcctgcc
     9001 catccgcggc gtacccgacc gcgccgtgca cgccgccctc gcagtcgccg aacaccttcg
     9061 agctcaacgc cggcgcgagc tggaagtgga tcagctacaa gctctccgtc tcgatgaccg
     9121 actacttcgg cgccaacgcg ggcaccggct actccggccg cacgcgtggc accatgtacc
     9181 acgacatcac ggtgacctat cccatcatgg acgacctgag cctcgcgggc cacgtgggcc
     9241 gcaccgacgt caaggggagc tacgccggcg cgaatccgga ctacaccgac tggcggctga
     9301 ccttgacgaa gacgttcacg ggcggctgga gcgcggccgt cgccggtgcc ggcgcgaaca
     9361 acgaccgctt ctaccgtccg ccggtcggcg gcctgtcgta cgccaacgcc ggcacccgcg
     9421 atctcaacaa gcccacgctg atcgtgcagc tcgggcgcgc cttctgaacc ggcgctccgt
     9481 gaccgacagc cgccccgacc ccgacgcgct cctggcccag ctgaaggccg aggaagcgca
     9541 ggcgcgccgc ggcaggctga agatcttctt cggcgccacg gcgggcgtcg gcaagacgta
     9601 tgcgatgttg tcggccgcgc gggcgcagcg ggcgcaggac gtcgacctgg tcgtcggggt
     9661 ggtcgagacg cacggccgcc tggagaccca gtcgctcctg gacggcctgg aggtggtgcc
     9721 gctgcgcgac gtgagctacc gcggccatgc gctcaaggag ttcgacctcg acgccgtgct
     9781 cacgcgcaag ccctcgctcg tgctggtcga cgagctcgcg cactccaacg tgcagggctc
     9841 gcgccacccg aagcgctggc aggacgtgca ggagctgctg gccgccggca tcgacgtctg
     9901 gacgacggtc aacgtccagc acctggagac gctcaacgac gtcgtcaacg gcatcacggg
     9961 cgtgcgggtg tgggagacgg ttccggaccg cgtgttcgac gaggccgacg aggtcgtcat
    10021 cgtcgacctg ccgcccgacg acctgctgca gcgcctgagg gacggcaagg tctacatgcc
    10081 gcagcaggcc gccaccgcgg tgcgcaactt cttccgcaag ggcaacctga tcgcgctgcg
    10141 cgagctcgcg ctgcgccgca ccgccgaccg cgtcgacgac gacatgctgc actggcgccg
    10201 cgcgcgcgcg gtcgcgccgg tctggcagac gcgcgaggcg atgctggtgt gcatcggccc
    10261 cgacgaacgc gccgacaagc tcgtgcgcac gggggcgcgg ctggcggccc agctggacgt
    10321 tccctggcat tgcgtctacg tcgagacccc gcgcctgcag cgcctgccgc cggcgcgccg
    10381 cgagagggtg ctgcggctgc tgcgcgtcgc ccaggaatcg ggtgccgaga ccgccgtcct
    10441 ggcgggcagc cagctggccg cggccatcgt gcgctacgcc cacgagcaca acctgccgcg
    10501 cgtcgtcctc gggcgggacg tgcggcaggt cgcgcgccgg tggcgaacgt cgctggcgga
    10561 cgccgtcggc acgatgggcg cggacctcga cgtcctgcag gtcgcgctgc cgcgcgcgaa
    10621 cggcgac
//
LOCUS       DASVLN010000252         4768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_120128, whole genome shotgun sequence.
ACCESSION   DASVLN010000252 DASVLN010000000
VERSION     DASVLN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120128"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VF453_05805"
     CDS             complement(<1..414)
                     /locus_tag="VF453_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX7687197.1"
                     /translation="MRTPAFLRFPLLTTAAAITAGLALGGCSSFSNEPATVKGFLAWI
                     APYKPDIVQGNVVTTEQISQIKPGMTRIQVRDILGTPLITDPFHAQRWDYVFTFKRQG
                     FDEQKRAFVVVFDKDAVEKIDAPSLPSEDEFVASIS"
     gene            519..971
                     /gene="fur"
                     /locus_tag="VF453_05810"
     CDS             519..971
                     /gene="fur"
                     /locus_tag="VF453_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEX7687198.1"
                     /translation="MTHAEELKSSGLKATLPRIKILEVFQRSAERHLTAEDVYKALLG
                     DGSDIALATVYRVLMQFEQAGLLARSHFESGKSVFELNEGTHHDHLLCLTCGRVEEFY
                     DAEIEARQREVASSRGFALQEHTLSLHAVCTRENCEHRPPGKPVGRHG"
     gene            complement(1110..2063)
                     /gene="hprK"
                     /locus_tag="VF453_05815"
     CDS             complement(1110..2063)
                     /gene="hprK"
                     /locus_tag="VF453_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX7687199.1"
                     /translation="MKPRVISAEALFEEHQPALRWEWIAGHAHPERRFDEAAVREARS
                     AADLIGYLNYIHPYRVQLVGRREVAYLANAPDDVQQRRIQRIVTLEPPVIIVADDERP
                     PDKLVALCDRAEIPLFVTSSSAGHVIDLVRSYLSQLFADRTTRHGVFMDILGVGVLLT
                     GESGLGKSELGLELISRGNGLVADDAVDLYRVSQSTLEGRCPDLLMNLLEVRGIGLLD
                     IRAIFGETAVRRKMRLKLIVHLVRKETMEREFERLPYEPLYEDILGIPVRKVVIAVDA
                     GRNLAVLVEAAVRNTILQLRGIDTYAEFVKRHQDAMARGGE"
     gene            complement(2191..2661)
                     /locus_tag="VF453_05820"
     CDS             complement(2191..2661)
                     /locus_tag="VF453_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7687200.1"
                     /translation="MNRLAAILPLANVQVGVDATSKKRVFEQAGVLFENQHAIARATV
                     TDNLFARERLGSTGLGHGVAVPHGRVKGLKNPLAAVLRVSQPIPFDAPDDEPVGLLIF
                     LLVPEAATQRHLEILSEIAEMLSDRALRDRLKGDVDAPTLHRLISDWEPLKSVA"
     gene            complement(3001..3354)
                     /gene="raiA"
                     /locus_tag="VF453_05825"
     CDS             complement(3001..3354)
                     /gene="raiA"
                     /locus_tag="VF453_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX7687201.1"
                     /translation="MNLTISGHHLEVTPALREYVLTKLDRITRHFDAVVDVSVLLTVE
                     NLKEKERRQKAEVTLHVKGKDIFVEQSHEDLYAAIDQLMDKLDRQVCRHKDRLQDHHH
                     ASGKRQLAAEQPAST"
     gene            complement(3591..>4768)
                     /locus_tag="VF453_05830"
     CDS             complement(3591..>4768)
                     /locus_tag="VF453_05830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7687202.1"
                     /translation="AAARAALHLLGRLRVGTLVIQAPDGTVHQAVGREGAEPHAALAV
                     HDWAMFAATMRAGDIGFAESFMAGQWTTPHLPDLLRLLLANREAIEQAIYGSRWGALL
                     HRVLHALRRNTRAGSRRNIQAHYDLGNAFYRLWLDPTMNYSSAWFEGDLGKPLAEAQT
                     AKLRRAIEATGLRAGQRLLEIGCGWGAVAESACAVDAAQVVGVTLSHEQLAWARDRLA
                     RSGHAARADLRLQDYRDVDDGPFDAVVSIEMFEAVGREYWDTYFRAVHRQLKPGAKAC
                     IQAIVIRDDLFERYAATPDFIQRHVFPGGMLASDALFVRHAAAAGLRVVDRFAFGRDY
                     AETLRRWRHAFLARVDEVRTQGFDERFVRLWEFYLAYCEAAFDAGNTDVVQYTLQRD"
BASE COUNT          722 a         1599 c         1707 g          740 t
ORIGIN      
        1 gctgatcgag gcgacgaatt catcctcgct gggcagggac ggggcgtcga tcttctcgac
       61 ggcgtccttg tcgaagacga ccacgaaggc gcgcttctgc tcgtcgaagc cctggcgctt
      121 gaacgtgaag acatagtccc agcgctgcgc gtggaacggg tcggtgatca gcggcgtgcc
      181 gaggatgtcg cgcacctgga tgcgcgtcat gccgggcttg atctggctga tctgctccgt
      241 cgtgacgacg ttgccctgca cgatgtcggg cttgtacggc gcgatccagg cgaggaagcc
      301 cttgaccgtg gcgggctcgt tgctgaagct gctgcagcca ccgagggcga ggcccgcggt
      361 gatcgccgcg gccgtcgtca ggagaggaaa gcgaaggaaa gccggagtgc gcatcgacga
      421 agctcggggc cgggccccca agggcgccaa ggctggatat gattcaccga ttctagtctg
      481 cgacggcaag tgcgccgcgg gcaaaagccg cacttaacat gacccacgcc gaagaactca
      541 agagcagcgg cctgaaagcc accctgccgc gcatcaagat cctcgaggtc ttccagcgtt
      601 ccgccgagcg gcacctgacg gccgaggacg tctacaaggc gctgctcggc gacggctcgg
      661 acatcgcgct ggccaccgtc taccgcgtgc tgatgcagtt cgagcaggcg ggcctgctcg
      721 cgcgcagcca tttcgagtcc ggcaagtcgg tcttcgagct caacgagggt acccaccacg
      781 accacctgct gtgcctgacc tgcggccgcg tcgaggagtt ctacgacgcc gagatcgagg
      841 cgcgccagcg cgaggtcgcg tcctcgcgcg ggttcgcgct gcaggagcac acgctgtcgc
      901 tgcacgccgt gtgcacgcgc gagaactgcg agcaccgccc gcccggcaag cccgtcggca
      961 gacatggttg acccccacgg tcgctggcgc tccctgcccc ccgagggggc gtgcgcgagc
     1021 agaccggcaa agccggatct gccgcttccc cgtgtctcgc ttggacgagc acaagcggca
     1081 gcgccgtcga aattggcgct gcgcgctact cactcgccgc cgcgggccat cgcgtcctgg
     1141 tggcgcttga cgaactcggc gtaggtgtcg atcccgcgca gctgcaggat cgtgttgcgc
     1201 accgccgcct cgaccagcac cgcgaggttg cggcccgcgt ccaccgcgat cacgaccttg
     1261 cgtaccggga tgcccaggat gtcctcgtac agcggctcgt agggcaggcg ctcgaactcg
     1321 cgctccatcg tctccttgcg cacgaggtgg acgatcagct tcagccgcat cttccgccgc
     1381 accgcggtct cgccgaagat cgcgcggatg tcgagcaggc cgatgccgcg cacctcgagc
     1441 aggttcatca gcaggtcggg gcagcggccc tcgagcgtcg actgcgagac gcggtagagg
     1501 tcgacggcat catcggcgac caggccgttg ccgcgcgaga tcagctcgag cccgagctcg
     1561 ctcttgccga ggccggactc gcccgtcagc agcacgccga cccccaggat gtccatgaac
     1621 acgccatggc gcgtcgtgcg gtcggcgaag agctggctca ggtacgagcg caccaggtcg
     1681 atcacgtggc ccgccgacga cgacgtcacg aacagcggga tctcggcgcg gtcgcacagc
     1741 gcgaccagct tgtccggcgg gcgctcgtcg tcggcgacga tgatgaccgg cggctccagc
     1801 gtcacgatgc gctggatgcg gcgctgctgg acgtcgtcgg gcgcgttggc gaggtaggcc
     1861 acctcgcggc gcccgaccag ctgcacgcgg tacgggtgga tgtagttgag gtagccgatc
     1921 aggtcggcgg ccgagcgcgc ctcgcgcacc gcggcctcgt cgaagcggcg ctccgggtgg
     1981 gcgtggccgg cgatccactc ccagcgcagc gcgggctggt gctcctcgaa cagcgcctcc
     2041 gcgctgatga cacggggttt catggagcct ccacggtcgc tggcgcgggg ccgtgcgcgt
     2101 gcagaccggc agagccggat ctgccgcttc ccggtgtctc gtttgctgcg tccgagcggc
     2161 agcgccggtg tcgactaggc atcctggcga tcaggcgacc gacttcagcg gttcccagtc
     2221 ggagatgagc cgatgcagcg tcggcgcgtc gacatcgccc ttgaggcggt cgcgcagcgc
     2281 gcggtcggac agcatctcgg cgatctcgga caggatctcg agatggcgct gcgtggcggc
     2341 ctcgggcacg agcaggaaga tcagcaggcc gaccggctcg tcgtccgggg cgtcgaacgg
     2401 gatcggctgc gagacgcgca gcaccgcggc gagcgggttc ttcaggccct tgacgcggcc
     2461 gtgcgggacg gcgacgccgt ggcctaggcc ggtcgagccc agtcgctcgc gggcgaacag
     2521 gttgtcggtg accgtcgcgc gcgcgatcgc gtgctggttc tcgaacagga cgcccgcctg
     2581 ctcgaagacg cgcttcttgc tggtggcgtc cacgcccacc tggacgttgg cgaggggaag
     2641 gatggcagcg agacggttca tgaagcgctc ccggcgggtg gtcgggatcc ggagtcaccc
     2701 ggtggtcccc gggctggagg tcacccggcg actcccggga tcgaacattc gattatagga
     2761 atcggcaaac cccgtgcgcg cgccgagagg aaggggtttg ccttggggcg cgaggattgc
     2821 tgcggcgcag caacaatttc acggattcgg gcccctgaag ccggtttcgg ccacgccggg
     2881 tcgcgcttga gcgtctcgcc aaaaaccatg tgcaggactc gacaaacgaa aaagcggccc
     2941 gcgggccgct tgctcgtcga gccggcgagg cgccgttgcc ggcgcatcgc ggcgcgggat
     3001 tcaggtcgag gccggctgct cggcggcgag ctggcgcttc ccggacgcgt gatgatggtc
     3061 ctgcagccga tccttgtggc ggcagacctg gcggtccagc ttgtccatca gctggtcgat
     3121 cgcggcgtag aggtcctcgt gggactgttc gacgaagatg tcctttccct tcacatgcag
     3181 ggtcacttcg gccttctggc gacgttcctt ttccttgagg ttttccacgg tgagcaacac
     3241 gctgacatcg acgacggcgt cgaaatggcg cgtgatcctg tccagcttgg tcaggacgta
     3301 ttcacgcagg gccggtgtca cttcgagatg atggccgctg atcgtcaggt tcatcagtgc
     3361 ctcctttcac gcgagagttt caagacatgg ccgggctcgc gagagcctgc cagcagggcc
     3421 ccggttcacc gttgggagga cccggggtgc gagtccacta tgcgcctcgg ccaggccttt
     3481 gaccagcctc cccaaatcgc gattagcggg ttatattcct tcgctgtcgg gggatcgccg
     3541 cgcgaccgtg cgtgctgcac ggccgaggtt gcgacgggcg gcacgcgcgc tcaatcgcgc
     3601 tgcagcgtgt actgcacgac gtcggtgttg ccggcgtcga acgcggcctc gcaataggcc
     3661 aggtagaact cccacagccg cacgaagcgc tcgtcgaagc cctgggtgcg cacctcgtcc
     3721 acgcgtgcga ggaaggcgtg gcgccagcgg cgcagcgtct cggcgtagtc gcggccgaac
     3781 gcgaagcgat cgacgacccg caggccggcg gcggcggcgt ggcgcacgaa cagcgcgtcg
     3841 ctcgccagca tgccgcccgg gaagacgtgg cgctggatga agtcgggcgt ggcggcgtag
     3901 cgctcgaaga ggtcgtcgcg gatcacgatc gcctggatgc acgcctttgc gcccggcttg
     3961 agctggcggt gcacggcgcg gaagtaggtg tcccagtact cgcggccgac ggcctcgaac
     4021 atctcgatcg agacgacggc gtcgaacggt ccgtcgtcga cgtcgcggta gtcctgcagg
     4081 cgcaagtcgg cgcgcgcggc atgtccggag cgcgcgagac gatcgcgcgc ccacgcgagc
     4141 tgttcgtgcg acagcgtcac gcccaccacc tgcgcggcgt cgaccgcgca ggcgctctcg
     4201 gccacggcgc cccagccgca gccgatctcc agcaggcgct ggccggcgcg caggccggtg
     4261 gcctcgatcg cgcggcgcag cttggcggtc tgcgcctcgg ccagcggctt gccgaggtcg
     4321 ccctcgaacc acgcgctgga gtagttcatc gtcggatcca gccacaggcg gtagaaggcg
     4381 ttgccgaggt cgtagtgcgc ctggatgttt cggcggctgc ccgcgcgcgt gttgcggcgc
     4441 agcgcgtgca ggacgcggtg cagcagcgcg ccccagcgcg agccgtagat cgcctgctcg
     4501 atggcctcgc gattggccag cagcagccgc agcaggtccg gaaggtgcgg cgtcgtccac
     4561 tggccggcca tgaagctctc ggcgaagccg atgtcgccgg cccgcatcgt cgcggcgaac
     4621 atcgcccagt cgtggaccgc gagcgcggcg tgcggctcgg cgccctcccg gccgacggcc
     4681 tggtggaccg tgccgtccgg cgcctggatg acgagcgtgc ccacccgcag ccggccgagc
     4741 aggtgcagcg ccgcccgggc ggccgcgg
//
LOCUS       DASVLN010000253         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_120281, whole genome shotgun sequence.
ACCESSION   DASVLN010000253 DASVLN010000000
VERSION     DASVLN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120281"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF453_05835"
     CDS             complement(<1..237)
                     /locus_tag="VF453_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687203.1"
                     /translation="MKSWVRWEGWPARPADAFDAAVEPRRRKRVLSRVDDLRAAPRLF
                     KHPARPPTPNDDGEPKLPVPGGAARPNAPAAVRPR"
     gene            250..2130
                     /gene="htpG"
                     /locus_tag="VF453_05840"
     CDS             250..2130
                     /gene="htpG"
                     /locus_tag="VF453_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX7687204.1"
                     /translation="MDKQTLSFQAEVQQILHLVTHSLYSNKEIFLRELVSNASDACDK
                     LRFEAIDHPQFLEDAPNLEVRLIVDAAARTLTIRDNGIGMSAEEAVAHLGTIAKSGTR
                     EFMSKLEGDQKKDANLIGQFGVGFYSGFIVADRITVESRRAGLPADQGVRWTSEGTGA
                     FEVETIEKAERGTDVILHLRADEDEFLRPWKLKSIVSKYSDHISLPILMKKEEWDADK
                     AEYKATDEWETVNKAAALWARSKSDVTDAEYQEFYKQISYDSDPPLAYTHNRVEGRSE
                     YTQLLYIPAKAPFDLWNRDKRGGVKLYVKRVFIMDDAEALMPVYLRFVKGVIDSADLP
                     LNVSRELLQESRDVKAIREGSTKRVLSMLESLADSDSAEDKDKYQAFWDEFGAVLKEG
                     IGEDHANRERLAKLYRFASTHADKGVSLADYVSRMKEGQEAIYVITADSLPAAKASPQ
                     LEIFRKKGIEVLLLVDRVDEWLLSHLMEFEGHALQSVAKGAVDLGKLQDEAEKKAAEE
                     AATAFKPTLDRLKEALKDRAKDVRATSRLVDSAACIVVEEGEMSAHLARLLKQAGQNA
                     PDAKPILEVNPEHPLVKKIESDPRFDDFAHLLFDQAWLAEGGQLEEPASYVQRVNRLL
                     VG"
     gene            2345..2974
                     /gene="queE"
                     /locus_tag="VF453_05845"
     CDS             2345..2974
                     /gene="queE"
                     /locus_tag="VF453_05845"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEX7687205.1"
                     /translation="MYSVKEIFYTLQGEGANAGRPAVFCRFAGCNLWTGREEDRATAA
                     CNFCDTDFVGTDGLGGGKFRTADLLADEIAQHWGAEPRHRFVVLTGGEPLLQVDKPLT
                     DALHARGFAIAVETNGTVPRPEGLDWVCVSPKADNALEILAGDELKLVFPQDGVDPAR
                     FEGLAFTHFFLQPMDNVLQSTNQRLAMQYCLAHPQWKLGLQTHKILDIR"
     gene            2977..3420
                     /gene="queD"
                     /locus_tag="VF453_05850"
     CDS             2977..3420
                     /gene="queD"
                     /locus_tag="VF453_05850"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEX7687206.1"
                     /translation="MLTITKRLEFDAGHRIPQHKSQCRNLHGHRYALEITLEGEPVDD
                     PGSSDHGMLMDFSDVKAIAKEHVVDPWDHAFIVHAGDTQVRELLDRIPGHKTVVVDRV
                     PTAENLVAIAFDILDPLYTARFGNGLVLKKLRLFETPTSWADCER"
BASE COUNT          635 a         1337 c         1262 g          507 t
ORIGIN      
        1 gcgcggccgc acggcggcgg gcgcgttcgg gcgcgccgcg ccgcccggca cggggagctt
       61 cggctccccg tcgtcgttgg gggttggcgg gcgcgccgga tgcttgaaaa gccggggggc
      121 ggccctcaag tcgtcgacgc gcgacaatac gcgtttgcgc cgccgcggct cgaccgccgc
      181 gtcgaaggcg tccgccggcc gggcgggcca cccctcccat cgaacccagg acttcaatcg
      241 ggcaacccca tggacaagca aaccctttcc ttccaggccg aggtccagca gatcctgcac
      301 ctcgtcacgc actcgctgta ctccaacaag gagatcttcc tgcgcgagct ggtctccaac
      361 gcgtccgacg cctgcgacaa gctgcgcttc gaggccatcg accacccgca gttcctcgag
      421 gacgcgccga acctggaggt gcgcctgatc gtcgacgccg cggcgcgcac gctgaccatc
      481 cgcgacaacg gcatcggcat gtcggccgag gaggcggtcg cccatctcgg cacgatcgcc
      541 aagagcggca cccgggagtt catgtcgaag ctggaaggcg accagaagaa ggacgccaac
      601 ctgatcggcc agttcggcgt cggcttctac tcgggcttca tcgtcgccga ccgcatcacg
      661 gtcgagtcgc gccgcgccgg cctgccggcc gaccagggcg tgcgctggac gtcggagggc
      721 acgggcgcct tcgaggtcga gaccatcgag aaggccgagc gcggcaccga cgtcatcctg
      781 cacctgcgcg ccgacgagga cgagttcctg cgcccgtgga agctgaagtc catcgtctcc
      841 aagtactccg accacatctc gctgcccatc ctgatgaaga aggaggaatg ggacgccgac
      901 aaggccgagt acaaggcgac cgacgagtgg gagacggtca acaaggcggc cgcgctgtgg
      961 gcgcgcagca agagcgacgt caccgacgcc gagtaccagg agttctacaa gcagatcagc
     1021 tacgacagcg acccgccgct cgcctacacg cacaaccgcg tcgagggccg cagcgagtac
     1081 acgcagctgc tctacatccc ggccaaggcc ccgttcgacc tctggaaccg cgacaagcgc
     1141 ggcggcgtga agctctacgt caagcgcgtg ttcatcatgg acgacgccga ggcgttgatg
     1201 ccggtctacc tgcgcttcgt caagggcgtg atcgactcgg ccgacctgcc gctgaacgtc
     1261 tcgcgcgagc tgctgcagga aagccgcgac gtcaaggcga ttcgcgaggg ctcgaccaag
     1321 cgcgtgctct cgatgctgga gtcgctggcc gacagcgaca gcgccgagga caaggacaag
     1381 taccaggcct tctgggacga gttcggcgcc gtgctcaagg agggcatcgg cgaggatcac
     1441 gccaaccgcg agcgcctggc caagctctac cgcttcgcgt cgacgcatgc ggacaagggc
     1501 gtctcgctgg ccgactacgt ctcgcgcatg aaggaaggcc aggaggcgat ctacgtcatc
     1561 acggccgact cgctgcccgc cgcgaaggcg agcccgcagc tcgagatctt ccgcaagaag
     1621 ggcatcgagg tgctgctgct ggtcgaccgc gtcgacgagt ggctgctgtc gcacctgatg
     1681 gagttcgagg gccacgcgct gcagagcgtg gccaagggcg ccgtcgacct gggcaagctc
     1741 caggacgagg ccgagaagaa ggccgccgag gaggccgcca ccgcgttcaa gccgaccctc
     1801 gaccgcctga aggaggcgct caaggatcgc gccaaggatg tccgcgccac ctcgcgcctg
     1861 gtcgattccg cggcttgcat cgtcgtcgag gagggcgaga tgagcgcgca cctcgcgcgg
     1921 ctgctcaagc aggccggcca gaacgccccg gatgccaagc ccatcctcga ggtcaacccc
     1981 gagcacccgc tcgtcaagaa gatcgagtcg gatccgcgct tcgacgactt cgcgcacctg
     2041 ctgttcgacc aggcctggct cgccgagggc ggccagctcg aggagccggc gtcctacgtg
     2101 cagcgcgtca accgcctgct ggtcggctga caaatggctg ttcctgcctt tcggcaaccg
     2161 ccgctggcgc ttacacgccg gcgtgaagtt cgggggagcg acggatccgg ctttgccggg
     2221 ccgtacgcgg ccgcccccct cgggggagcg caggcggcaa agggctcgcg tgagcgagac
     2281 cccgccggag cggctcgcgg catgcttgcc gcgagccccg gagcgccagc gagcgtgtgg
     2341 gcacatgtat agcgtcaagg aaatcttcta cacgctccag ggcgagggcg cgaacgcggg
     2401 ccggccggcg gtgttctgcc gcttcgccgg ctgcaacctg tggacggggc gcgaggagga
     2461 tcgcgccacg gccgcctgca acttctgcga taccgacttc gtcggcaccg acggcctggg
     2521 cggcggcaag ttccgcaccg ccgacctgct ggccgacgag atcgcgcagc actggggcgc
     2581 ggagccccgg catcgcttcg tcgtgctgac cggcggcgag ccgctgctgc aggtcgacaa
     2641 gccgctgacc gacgccctgc atgcgcgcgg cttcgcgatt gcggtcgaga ccaacggcac
     2701 cgtgccgcgg cccgaagggc tcgactgggt ctgcgtgagc ccgaaggcgg acaacgcgct
     2761 cgagatcctg gccggcgacg agttgaagct ggtctttccg caggacggcg tcgacccggc
     2821 gcgcttcgag ggactcgcct tcacgcactt cttcctgcag ccgatggaca acgtgctgca
     2881 gtcgaccaat cagcgcctgg cgatgcagta ctgcctcgcg cacccgcaat ggaagctggg
     2941 actgcagacg cacaagatcc tggacatccg ctgaccatgc tcaccatcac gaaacgcctc
     3001 gagttcgacg cgggccaccg catcccccag cacaagagcc agtgccgcaa cctgcacggg
     3061 caccgctacg cgctggagat cacactcgag ggcgagcccg tcgacgaccc gggctcgagc
     3121 gaccacggca tgctgatgga cttcagcgac gtcaaggcga tcgccaagga gcacgtggtc
     3181 gacccgtggg accacgcgtt catcgtgcat gccggcgaca cgcaggtgcg cgagctgctc
     3241 gaccggatcc ccggccacaa gaccgtcgtc gtcgaccgcg tgccgacggc cgagaacctc
     3301 gtcgcgatcg cattcgacat cctcgacccg ctgtataccg cgcgcttcgg caacggcctc
     3361 gtgctgaaga agctgcggct gttcgagacg cccacgagct gggccgactg cgaacgctga
     3421 acgacggccc ccgctcgctg cgctcaccgc cccccgaggg ggctgggccg ttcgctccgg
     3481 aaacgacgac ccccgctcgc tgcgctcacc gcccccgagg ggggctgggc cgctcgctcc
     3541 cgaaacgacg acccccgctc gctgcgctcg ccgccccccg agggggctgg gccgctcgct
     3601 ccggaaacga cgacccccgc tcgctgcgct caccgccccc cgagggggct gggccgctcg
     3661 ctccggagcg gccgagcgct cgcggccctg gcccgcgcgc ggcgcagggt ctcatccctc
     3721 gtaggcagcc aggcggcgct g
//
LOCUS       DASVLN010000254         4685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_497586, whole genome shotgun sequence.
ACCESSION   DASVLN010000254 DASVLN010000000
VERSION     DASVLN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497586"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..2931
                     /gene="ileS"
                     /locus_tag="VF453_05855"
     CDS             58..2931
                     /gene="ileS"
                     /locus_tag="VF453_05855"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX7687207.1"
                     /translation="MTADTTDHPKSTPDYRATLNLPDTPFPMRGDLPRREPGWVKQWD
                     EEGRYKRLRDAREGRPLFVLHDGPPYANGQIHMGHAVNKVLKDMIVKTRQLMGFDAQY
                     APGWDCHGLPIENAIEKEFGRKLSRDEMQSKSRAYATAQIGQQMVDFKRLGVLGDWEH
                     PYRTMDFANEAGELRAFKRVIERGFVYRGLKPVYWCFDCGSSLAEFEIEYQDKKSYTI
                     DVGFKCAEPQKLAAAFGLPALAKDAFVVIWTTTPWTIPANQALNLNPQLEYSLVDTDR
                     GLLLLASALVEKCLARYKLEGRVVGTALGQALDGLKFRHPLAEVDPGYDRLSPVYLAD
                     YATADDGTGIVHSSPAYGVDDFNSCVAHGVKIDEILNPVQGSGVYAPELPLFGGQFIW
                     KAQPAIIEALRAAGRLFDTSEIVHSYPHCWRHKTPVIYRAAAQWFVRMDEGEGVFTKD
                     KAPRTLRQMALAAIDQTGFYPENGKARLRDMIANRPDWCISRQRSWGVPIPFFLHKDT
                     GELHPRTMEILDQAADIVEKGGIEAWSRVTPEEILGADDAPAYTKSTDILEVWFDSGS
                     TFFHVLRGTHKGNEAHPGFHPQGPEADLYLEGHDQHRGWFHSSLLLACALYGRAPYKG
                     LLTHGFTVDSQGRKMSKSLKNGVDPQETSGKLGAEIIRLWVAASDYSGDIAGDDKILA
                     RVVDAYRRIRNTLRFLLANTSDFDPRRDAVPLADLVEVDRYALARAAQLQADVLAHYE
                     KYEFHPVVAKLQVYCSEDLGAFYLDVLKDRLYTTAPKSFARRSAQTALWHITHAMLRW
                     MAPFLSFTAEEAWPVFAPGASFSIFAETFWPLEQGDAALLAKWERLRAIREAANKAIE
                     EVRAAGGVGSSLQAELVIAANAEDRVLLESLGDDLKFVTITSRVELAAADALAVAVTP
                     SSSPKCERCWHWRDDVGRDPAHPTLCGRCTSNLFGTGEVRTAA"
     gene            3187..3708
                     /gene="lspA"
                     /locus_tag="VF453_05860"
     CDS             3187..3708
                     /gene="lspA"
                     /locus_tag="VF453_05860"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX7687208.1"
                     /translation="MARSPARYSSRSSSAGGPSLWLWLGLALVVVLLDQFAKALIVGD
                     FREYESRRVTSFFNVVRWHNHGAAFSFLNGAGGWQRWMFIGLAIAASVFIVWLLRRHA
                     SQRLFCFAIAMVLGGAVGNVIDRIVRGYVVDFISLHWKDVYAFPAFNLADSAITLGAI
                     CLVVDELRRVRRS"
     gene            3713..4522
                     /locus_tag="VF453_05865"
     CDS             3713..4522
                     /locus_tag="VF453_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7687209.1"
                     /translation="MARLTAAALLLVLAAGAAQADPAPPAGLDAVDLEAMTSTELRER
                     VAHGATTILVPIGGTEQNGAHMVLGKHNVRAHVLADRIARELGNAIVAPTIAYVPEGS
                     IHPPAAHMKYAGTISIPDAVFEGLLEAAARSFKQHGFKDVVLLGDHGGYQADLARVAD
                     KLDREWARDPSCRVHAPPEYYRVTQTAYVQALKAHGATDAELGVHAGLADTSLALAVD
                     PALVREDKLAGPYPPGVTGNPRRSTAALGELGTKLVVEATVAAIRNETKPR"
BASE COUNT          710 a         1747 c         1560 g          668 t
ORIGIN      
        1 cgccgcgacg gctcttcagc acctcccgcg cgcgccgccg ccggcgcgct ctccgccatg
       61 accgctgaca cgaccgacca cccgaagtcc acgccggact accgcgccac gctcaacctg
      121 cccgacacgc cgttcccgat gcgcggggac ctgcccaggc gcgagccggg atgggtcaag
      181 caatgggacg aggaaggccg ctacaagcgc ctgcgcgacg cgcgcgaggg ccgcccgctg
      241 ttcgtcctgc atgacggccc gccctacgcc aacggccaga tccacatggg ccacgccgtg
      301 aacaaggtgc tcaaggacat gatcgtcaag acccgccagc tgatgggctt cgatgcccag
      361 tacgcgccgg gctgggactg ccacggcctg ccgatcgaga acgcgatcga gaaggaattc
      421 ggccgcaagc tgagccgcga cgagatgcag tcgaagagcc gcgcctacgc gaccgcgcag
      481 atcggccagc agatggtcga cttcaagcgc ctgggcgtgc tcggcgactg ggagcatccg
      541 taccgcacga tggacttcgc caacgaggcc ggcgagctgc gcgccttcaa gcgggtgatc
      601 gagcgcggct tcgtctaccg cggcctgaag cccgtgtact ggtgcttcga ctgcggctcg
      661 tcgctggccg agttcgagat cgagtaccag gacaagaaga gctacacgat cgacgtcggc
      721 ttcaagtgcg cggagccgca gaagctggcg gccgccttcg ggctgccggc gctggccaag
      781 gacgccttcg tcgtcatctg gacgaccacg ccgtggacga tccccgccaa ccaggcgctc
      841 aacctcaacc cgcagctcga atacagcctc gtcgacaccg accgtggcct gctgctgctg
      901 gcgtcggcgc tcgtcgagaa gtgcctggcg cgctacaagc tggagggccg ggtcgtgggc
      961 acggcgctcg ggcaggcgct ggacgggctg aagttccgcc acccgctggc cgaggtcgac
     1021 ccgggctacg accgcctgtc gcccgtctac ctcgccgact acgcgaccgc cgacgacggc
     1081 accggcatcg tccactcgtc gcccgcgtac ggcgtggacg acttcaactc ctgcgtcgcg
     1141 cacggcgtga agatcgacga gatcctgaac ccggtgcaag gctccggcgt gtacgcgccc
     1201 gagctgccgc tgttcggcgg ccagttcatc tggaaggcgc agccggccat catcgaggcc
     1261 cttcgcgcgg ccggccggct gttcgacacc tcggagatcg tccacagcta cccgcactgc
     1321 tggcgccaca agacaccggt gatctaccgc gccgccgcgc aatggttcgt gcggatggac
     1381 gagggcgaag gcgtcttcac gaaggacaag gcgccgcgca cgctgcgcca gatggcgctc
     1441 gcggcgatcg accagaccgg cttctatccc gagaacggca aggcacgcct gcgcgacatg
     1501 atcgccaacc gtcccgactg gtgcatcagc cgccagcgca gctggggcgt gccgatcccg
     1561 ttcttcctgc acaaggacac gggcgagctg cacccgcgca cgatggagat cctcgaccag
     1621 gcagccgaca tcgtcgagaa gggcggcatc gaggcctgga gccgcgtgac gcccgaggag
     1681 atcctcggcg ccgacgacgc gcccgcctac accaagagca ccgacatcct cgaggtctgg
     1741 ttcgactcgg gctcgacctt cttccacgtg ctgcgcggca cgcacaaggg caacgaggca
     1801 cacccgggct tccatcctca gggcccggag gccgacctct acctcgaggg ccacgaccag
     1861 catcgcggct ggttccactc gtcgctgctg ctggcctgcg cgctctacgg tcgcgcgccg
     1921 tacaagggcc tgctgacgca cggcttcacc gtggacagcc agggccgcaa gatgagcaag
     1981 tcgctcaaga acggcgtcga tccgcaggag accagcggca agctcggcgc cgagatcatc
     2041 cgcctctggg tcgcggcaag cgactactcg ggcgacatcg ccggggacga caagatcctc
     2101 gcgcgcgtgg tcgacgcgta ccgccgcatc cgcaacacgc tgcgcttcct gctcgccaac
     2161 acgagcgact tcgacccgcg gcgcgacgcc gtgccgctcg ccgacctggt ggaggtcgac
     2221 cgctacgccc tggcgcgcgc cgcgcagctg caggccgacg tgctcgcgca ctacgagaag
     2281 tacgagttcc acccggtcgt cgcgaagctg caggtctact gttccgagga cctgggcgcg
     2341 ttctacctcg acgtgctcaa ggatcgcctc tacacgacgg cgccgaagtc gttcgcgcga
     2401 cggtcggcgc agaccgcgct gtggcacatc acgcacgcga tgctgcgctg gatggcgcct
     2461 ttcctcagct tcaccgccga ggaggcgtgg cccgtcttcg cgccgggcgc gtcgttctcg
     2521 atctttgccg agaccttctg gccgctggaa cagggcgacg ccgcgctgct cgcgaaatgg
     2581 gagcgcctgc gcgcgatccg cgaggccgcc aacaaggcga tcgaagaggt gcgcgccgcc
     2641 ggcggtgtgg gctcgtcgct gcaggcggag ctcgtgatcg cggccaacgc cgaggatcgc
     2701 gtcctgctcg agtcgctcgg cgacgacctg aagttcgtga ccatcacgtc cagggtcgaa
     2761 ctcgctgccg ccgacgcgct cgccgtcgcg gtcacgccgt cctcgtcgcc gaagtgcgag
     2821 cgctgctggc actggcgcga cgacgtcggc cgcgatccgg cgcaccccac gctgtgcggg
     2881 cgctgcacga gcaacctgtt cggcacgggc gaggtgcgga cggcggcctg acgccgcgcc
     2941 gatggacgac gccgcttcga cgccaggcaa cgcggccgat ccggtgccga ggctcgctgc
     3001 cggcgcggcg ctcgacatcg gcaggcgcgg ctgcgggcgg gtgccgagcc tcgcgatcgg
     3061 cccccgtcgg tcggctaaag tagccggtcc cgcgcccggc cgagcgcctc gtcggcgtcc
     3121 cgaccggctc gcgtgaacgc gctcacctcc gcttccgatc cttcgcattc gatccttcat
     3181 ccctgcatgg cccgctcccc cgcccgatat tcgtcccgaa gcagcagcgc cggcggcccg
     3241 tccctgtggc tgtggctggg cctcgcgctc gtcgtcgtgc tgcttgacca gtttgccaag
     3301 gcgctgatcg tcggcgactt ccgcgaatac gagagccgcc gcgtcacctc gttcttcaac
     3361 gtcgtgcgat ggcacaacca cggcgcggcg ttctcgttcc tgaacggcgc gggcgggtgg
     3421 cagcgctgga tgttcatcgg gctggcgatc gcggcctcgg tcttcatcgt ctggctgctt
     3481 cggcggcatg cgtcgcagcg gctgttctgc ttcgcgatcg ccatggtgct gggcggcgcg
     3541 gtgggcaacg tgatcgaccg catcgtgcgc ggctacgtcg tcgacttcat ctcgctgcac
     3601 tggaaggacg tgtacgcctt cccagccttc aacctcgccg acagcgcgat cacgctgggc
     3661 gcgatctgcc tggtcgtcga cgagctgcgc cgcgtgcgcc ggagctgacg cgatggcgcg
     3721 cctcaccgcg gcagcccttc ttctcgtgct tgccgccggt gcggcgcagg ccgacccggc
     3781 gccgcccgcc ggcctcgacg ccgtcgacct ggaggcgatg accagcaccg agctgcgcga
     3841 gcgcgtcgcc cacggtgcga cgaccatcct cgtgcccatc ggcggcaccg agcagaacgg
     3901 cgcccacatg gtgctgggca agcacaacgt ccgcgcgcac gtcctcgccg atcgcatcgc
     3961 ccgcgagctg ggcaacgcga tcgtcgcgcc cacgatcgcc tacgtgcccg agggcagcat
     4021 ccacccgccc gcggcgcaca tgaagtacgc gggcacgatc tcgattccgg acgccgtctt
     4081 cgagggcctg ctggaggccg cggcgcgcag cttcaagcag cacgggttca aggacgtcgt
     4141 cctcctcggc gaccacggcg ggtaccaggc cgacctcgcg cgggtcgcgg acaagctcga
     4201 ccgcgagtgg gcgcgggatc cgtcctgccg cgtccacgcg ccccccgagt actaccgcgt
     4261 cacgcagacg gcctacgtgc aggcgctgaa ggcgcacggc gcgaccgacg ccgagctcgg
     4321 cgtgcacgcg gggctggccg acacctcgct cgcgctggcc gtcgatccgg cgctggtacg
     4381 cgaggacaag ctcgccgggc cctacccgcc gggcgtgacc ggcaacccgc ggcgctcgac
     4441 cgccgcgctg ggggaactcg gcacgaagct ggtcgtcgaa gccacggtcg ccgcgatccg
     4501 caacgaaacg aaaccccgct gatcggcgcg cggcgcccgc cggcatttgc ccgacaatcg
     4561 cagcagcccc cgcacgaaac gcctcccccg acatgacgcc cttcctccgc cgcggcctgc
     4621 tcgtcgccgc gcccgccctg cccgccgcgc tggcgctctt cgccgcgctg caccatcccg
     4681 tcgcc
//
LOCUS       DASVLN010000255         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_497615, whole genome shotgun sequence.
ACCESSION   DASVLN010000255 DASVLN010000000
VERSION     DASVLN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497615"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1613)
                     /locus_tag="VF453_05870"
     CDS             complement(<1..>1613)
                     /locus_tag="VF453_05870"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX7687210.1"
                     /translation="ALRDLRLPAPAVDGPAPPPGASAATLMLVCAIAAMLVVVGLVCS
                     WLDTHAHARNRQLADSLSEANRRLRDQAHFDALTRLPNRLMFEERMKAALEAAGARPE
                     PCSIAVLFIDLDGFKPINDSFGHVAGDAVLREIGRRLQAVARDGDTVARVGGDEFLMM
                     TPRPGHEAVAASVAQRLLEALNEPLTLPNDVTVNLSGSIGIVLYPEHGPASKLIANAD
                     AAMYAAKRAGGSTYAFFEPRMELDAREQLALQNELRHAIERGELQLYYQPKIDGRSGQ
                     ITGVESLLRWQHAVRGFVPPGLFIPIAERFGLIGAIGNWVIDEACRQLREWKRQGLRM
                     RVAVNLSVHQLRQEDLVQRVRVAVEHFGVDPQLLTFEITESVAMEDTQETMRAFAQLA
                     SVGVSLSIDDFGTGYSSLAYLRKLPARQLKIDQSFVQDLGTSPDALAVVDAVIRLAHA
                     LGLRVVAEGVETPRQRDILLGLRCDELQGYLFARPMTARMLTLWATGESHPGAHALGR
                     DDAGAAARADFRPSLFLEETDARAPGEAG"
BASE COUNT          222 a          556 c          622 g          213 t
ORIGIN      
        1 gccggcctcg ccgggcgcgc gggcgtccgt ctcctcgagg aagagcgacg ggcggaagtc
       61 cgcgcgcgcg gcggcgccgg cgtcgtcccg tcccagcgca tgcgcgcccg gatgggactc
      121 gcccgtcgcc cacagcgtca gcatacgcgc ggtcatcggc cgcgcgaaca ggtagccctg
      181 cagctcgtcg cagcgcaggc cgagcaggat gtcgcgctgg cgcggcgtct ccacgccctc
      241 ggcgacgacg cgcaggccca gcgcgtgcgc gagacggatc accgcgtcca cgaccgccag
      301 cgcgtcgggg ctggtgccga ggtcctgcac gaagctctgg tcgatcttga gctggcgcgc
      361 cggcagcttg cgcaggtagg cgaggctcga gtagccggta ccgaagtcgt cgatggacag
      421 cgacacgccc acgctcgcga gctgcgcgaa ggcgcgcatc gtctcctggg tgtcctccat
      481 cgcgaccgac tcggtgatct cgaaggtcag cagctgcggg tcgacgccga agtgctccac
      541 cgcgacgcgc acgcgctgga cgaggtcctc ctggcgcagc tggtgcaccg agaggttgac
      601 cgcgacgcgc atgcgcaggc cctggcgctt ccactcgcgc agctggcggc aggcctcgtc
      661 gatgacccag ttgccgatcg cgccgatcag gccgaagcgc tcggcgatcg ggatgaacag
      721 gccgggcggc acgaagccgc gcaccgcgtg ctgccagcgc agcagcgact cgacgcccgt
      781 gatctgcccg gagcggccgt cgatcttggg ctggtagtac agctgcagct cgccgcgctc
      841 gatcgcgtgg cgcagctcgt tctgcagcgc gagctgctcg cgcgcgtcga gctccatgcg
      901 cggctcgaag aacgcgtagg tcgagccgcc tgcgcgcttg gcggcgtaca tcgcggcgtc
      961 ggcgttggcg atcagcttgg aggcgggccc gtgctcgggg tagagcacga tgccgatcga
     1021 gcccgagagg ttgacggtga cgtcgttggg cagcgtcagc ggctcgttca gggcctccag
     1081 caggcgctgc gcgaccgagg cggcgacggc ctcgtggccg gggcgcgggg tcatcatcag
     1141 gaactcgtcg ccgccgacgc gcgccacggt gtcgccgtcg cgggcgacgg cctgcaggcg
     1201 gcggccgatc tcgcgcagca cggcgtcgcc ggcaacgtgg ccgaaggagt cgttgatcgg
     1261 cttgaagccg tccaggtcga tgaacagcac ggcgatcgag cacggttcgg gacgcgcgcc
     1321 ggcggcctcg agggcggcct tcatgcgttc ctcgaacatc agccggttcg gcaggcgggt
     1381 cagcgcgtcg aagtgggcct ggtcgcgcag gcggcggttg gcctcgctga gcgagtcggc
     1441 gagctggcgg ttgcgcgcgt gggcatgggt gtccagccac gagcacacga ggccgacgac
     1501 gaccagcatc gccgcgatcg cgcacaccag catcagcgtg gcggcgctcg cgcccggcgg
     1561 cggggcgggg ccgtcgacgg cgggcgcggg caggcgcagg tcgcgcagag ccg
//
LOCUS       DASVLN010000256         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_335410, whole genome shotgun sequence.
ACCESSION   DASVLN010000256 DASVLN010000000
VERSION     DASVLN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335410"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1233)
                     /locus_tag="VF453_05875"
     CDS             complement(7..1233)
                     /locus_tag="VF453_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7687211.1"
                     /translation="MTSHSDDTTNSPWHLAQRCARMNPSILREILKVTELPGVRSLAG
                     GLPAADTFPVEAMREATQRVLADTPREALQYAASEGFGPLREWVSQQMTAAGLDAPAE
                     RILILNGSQQGLDLVGRVLLDAGSAVAVETPTYLGALQAFTPNEPVFTSVASDADGPL
                     PEAVAALPRGTRFMYVLPNFQNPTGRVVPAARRDAIVAAAQRAGVPMLEDNPYGDLWY
                     DEAPPKPIASRWHEGTVYLGSFSKVFAPGLRLGYLVCPKPLFPKLLQAKQAADLHTTG
                     FNQRVVHQVIKDGFLEHHIPAIRNRYRVQRDAMAAALKAHLPAGCEWRAPAGGMFFWV
                     RLPEGFDAVALFPKAVEAGVAYVPGAAFYADAPDVRTVRLSFVTLSPADIEDAVSRLG
                     GVLRAHAATLPAAVAA"
     gene            complement(1280..2278)
                     /locus_tag="VF453_05880"
     CDS             complement(1280..2278)
                     /locus_tag="VF453_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7687212.1"
                     /translation="MRLEPQAATSSTAAHGPPAGACEAAHRHQERRTRLGLWLGVLGM
                     LIFAFTIPMTRLACGGGDSPQLDPVFVAIGRAGVAGLLSLAYLAATRAPWPHRSHWAM
                     LVLTLLGNVLGWPLLLGLAVRRVDAMHASVISGLMPLTTAAVGALLLRQRPSRGFWTF
                     ALLGAALVVGFAAWRGAGLPQLADLLLLGAITASSLGYITGVRVTQGPHALTPEQTIS
                     WVLVLGLPLTLPVLVLQWPTTTVHPAAWAGFAYVAVMSMWMGFFAWYRGLAWGGTLRV
                     SQVQLLQPFVSVLLSVPLLGERLEPATLAFSIAVMACVYFGRRMSTGAAGAVGGRP"
BASE COUNT          381 a          890 c          970 g          282 t
ORIGIN      
        1 cggcgatcat gccgccaccg ccgccggcag cgtcgccgcg tgcgcgcgca gcacgccgcc
       61 gaggcggctc accgcgtcct cgatgtcggc gggcgacagc gtgacgaagg acaggcgcac
      121 cgtgcgcacg tcgggcgcgt cggcgtagaa ggccgcgccg ggcacgtagg cgacgcccgc
      181 ctcgaccgcc ttcgggaaca gcgcgacggc gtcgaagccc tcgggcaggc gcacccagaa
      241 gaacatgccg cccgccggcg cgcgccactc gcagccggcc ggcaggtgcg ccttcagcgc
      301 cgcggccatc gcgtcgcgct ggacgcggta gcggttgcgg atcgcgggga tgtggtgctc
      361 caggaagcca tccttgatca cctggtgcac gacgcgctgg ttgaagccgg tcgtgtgcag
      421 gtcggccgcc tgcttggcct gcagcagctt ggggaagagc ggcttcggac agacgaggta
      481 gcccaggcgc agcccgggcg cgaacacctt ggagaacgag cccaggtaga cggtgccctc
      541 gtgccagcgc gaagcgatcg gcttgggcgg cgcctcgtcg taccagaggt cgccgtacgg
      601 gttgtcctcc agcatcggca cgccggcgcg ctgggcggcg gcgacgatcg cgtcgcggcg
      661 cgcggcgggc acgacgcggc ccgtcgggtt ctggaagttg ggcagcacgt acatgaagcg
      721 cgtgccgcgc ggcagtgcgg cgacggcctc gggcagcggg ccgtcggcgt cgctggccac
      781 cgacgtgaag accggctcgt tgggcgtgaa ggcctgcagc gcgccgaggt aggtcggcgt
      841 ctcgacggcg accgcgctgc cggcgtccag cagcacccgg ccgacgaggt cgaggccttg
      901 ctgcgacccg ttgaggatca ggatgcgctc ggccggcgcg tcgaggccgg cggcggtcat
      961 ctgctgcgag acccattcgc gcagcggccc gaagccctcg ctggccgcgt actgcagcgc
     1021 ctcgcgcggg gtgtcggcga gcacgcgctg cgtcgcctcg cgcatcgcct cgaccgggaa
     1081 ggtgtcggcc gcgggcaggc cgcccgcgag ggagcgcacg ccgggcagct cggtgacctt
     1141 caggatctcg cgcaggatcg acgggttcat gcgggcgcag cgctgcgcga ggtgccaggg
     1201 cgagttcgtc gtgtcgtcgg agtgcgaggt catggtcggg taccttggtg ggcttggatg
     1261 gaggggatcg taggtcaggt cacgggcggc cgccgaccgc gccggcggcg ccggtggaca
     1321 tgcggcggcc gaagtagacg caggccatca cggcgatcga gaaggcgagc gtggcgggct
     1381 cgaggcgctc gcccagcagc ggcacgctca ggagcacgga gacgaagggc tggagcagct
     1441 gcacctggct cacgcgcagc gtcccgcccc aggcgaggcc gcggtaccag gcgaagaagc
     1501 ccatccacat ggacatcacc gccacgtacg cgaagccggc ccaggcggcg ggatgcaccg
     1561 tggtcgtggg ccactgcagc acgagcaccg gcagcgtgag cggcagtccg agcacgagca
     1621 cccaggagat cgtctgctcc ggcgtcagcg cgtgcggccc ctgcgtcacg cgcacgccgg
     1681 tgatgtagcc gagcgaggac gcggtgatcg cgcccagaag cagcaggtcg gccagctgcg
     1741 gcaggccggc gccgcgccac gccgcgaagc ccaccacgag cgccgccccc agcagcgcga
     1801 aggtccagaa gccgcgcgag ggccgctggc gcagcagcag cgccccgacc gccgcggtgg
     1861 tcagcggcat cagcccgctg atgacggacg cgtgcatcgc gtcgacccgt cgcaccgcca
     1921 gcccgagcag cagcggccag ccgagcacgt tgcccagcag cgtcagcacg agcatcgccc
     1981 agtgcgagcg gtgcggccac ggcgcgcgcg tcgcggccag gtaggcgagc gacagcagcc
     2041 ccgcgacgcc ggcgcggccg atcgcgacga agacgggatc cagctgcggc gagtcgccgc
     2101 cgccgcaggc caggcgcgtc atcgggatgg tgaacgcgaa gatcagcatg cccagcacgc
     2161 cgagccacag gccgagccgg gtgcggcgct cctggtgccg gtgcgcggcc tcgcacgcgc
     2221 cggccggcgg accgtgggcg gcggtcgagg acgtggcggc ttggggttcg aggcgcatcg
     2281 cggcgtcggt caaggaaggc ggacgaccgg ccggagggct ggtcgggacg tccagtctag
     2341 gctcataatc aatacacgat cagtacagtt ctcgaatgca gagaccagat ctgtaccgac
     2401 gtcggcggca atacagatcc gccgtccggc cagacacacc cgctcgcccg catgccgcac
     2461 gatccgatgc ccgcccccga cgcccgcccg cgctcccgcg ccgccccggg cggggcgcac
     2521 gcg
//
LOCUS       DASVLN010000257         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_282330, whole genome shotgun sequence.
ACCESSION   DASVLN010000257 DASVLN010000000
VERSION     DASVLN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282330"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VF453_05885"
     CDS             complement(<1..311)
                     /locus_tag="VF453_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7687213.1"
                     /translation="MERSFRDLWNGRSGIAREVSMSGRPQYDEVAVIEAAARSFQRHG
                     YAATSLHELTAVTGLSRSSLYQRFRDKEGLFQQALAAYTTRLLRRMNAARDGSPRARL
                     RA"
     gene            complement(360..2135)
                     /locus_tag="VF453_05890"
     CDS             complement(360..2135)
                     /locus_tag="VF453_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX7687214.1"
                     /translation="MTLHQIESILIIAAMLAAFMSGRLSYDIVAGLALVVAVVLGVVP
                     VDKAFLGFANPVIVIIASVLVVSRAIAASNVLDVWIGSLLSRVKSTSLQIGVLTASVA
                     YLSAFVKNVGTLGIVMPLAIQTARRSGRPRSMYLMPLAFGSLVGGTITKIGTSPNLLI
                     STVRHELGRPPFGLFDFVAVGLPLTTLAVVFLAFGWRLLPEREGHKSAEEAFSIEAYT
                     TELRIPAQPASPGLTVRGLEEMGEGEMSVAAIVREGGRRYIPSEDWRLFPGDVVVVLA
                     DPTVVEKVVAKTGFEIHGAKDIPEPPEKMDEMEVMEVIVTADSLLVGATPASLQLRHR
                     FGVNLFAVRKASRASTTRLRAHRFAAGDVVLLQGWGKSLASSVAELGVLPLADRKLTL
                     GRRASGLVPIGLLAAALVLVGLGWVQVEVAFFAAAVLTVLLRQVTVKEAYAAIEWPVI
                     VMLACLIPVGEGLKETGATALIAHALTHVAGQVPGGVAVGVVVLAAMLLTPLLHHAAA
                     VLVLGPVAAAVAGDLGYRADPFLMAVALGCACDFLTPIGHQNNTLVMGPGGYRFGDYW
                     RLGLPLTLMVLFVGTPLILRAWPLH"
BASE COUNT          364 a          810 c          776 g          262 t
ORIGIN      
        1 gcccgcaggc gcgcgcgcgg cgagccgtcg cgcgccgcgt tcattcgccg cagcagccgg
       61 gtcgtgtagg ccgcgagagc ctgctggaac agcccctcct tgtcgcggaa gcgctggtag
      121 aggctcgagc gcgagagccc cgtgacggcc gtgagctcgt ggagcgaggt cgcggcgtag
      181 ccgtggcgct ggaacgaccg cgcggccgcc tcgatgaccg cgacctcgtc gtactgcggc
      241 cttccgctca tggacacctc ccgggcgatg ccggaacgac cgttccagag atcccggaac
      301 gaacgttcca tgaatgatgg atcgatcgtt ccatcatatc ggcgcgggcg gtgccaagtt
      361 cagtgcaggg gccaggcgcg caggatcagc ggcgtcccga cgaacaggac catcagcgtc
      421 agcggcagcc ccagccgcca gtagtcgccg aagcggtagc cgccgggccc catcacgagc
      481 gtgttgttct ggtggccgat gggcgtcagg aagtcgcagg cgcagccgag cgccaccgcc
      541 atgaggaagg ggtcggcccg gtagccgagg tcgcccgcga ccgccgcggc caccggcccc
      601 agcaccagaa cggcggcggc gtggtgcagc agcggcgtga gcagcatcgc ggccagcacc
      661 accacgccca ccgcgacgcc gcccggcacc tgccccgcca cgtgggtcag cgcgtgcgcg
      721 atgagcgcgg tcgcacccgt ctccttgagc ccttcgccga ccgggatcag gcaggcgagc
      781 atgacgatca ccggccactc gatcgcggcg taggcctcct tgacggtgac ctggcgcagc
      841 agcacggtca gcaccgcggc ggcgaagaag gcgacctcga cctgcaccca gcccagcccg
      901 accagcacca gcgccgcggc cagcagcccg atcggcacga ggccgctggc gcgccggccg
      961 agcgtgagct tgcggtcggc cagcggcagc acgccgagct cggccacgga cgacgcgagg
     1021 ctcttgcccc agccctgcag cagcacgacg tcgccggccg cgaagcggtg cgcgcgcagg
     1081 cgcgtggtcg acgcccgcga cgccttgcgc accgcgaaca ggttgacgcc gaagcggtgg
     1141 cgcagctgca gcgaggccgg cgtcgcgccg accagcagcg agtcggcggt gacgatgacc
     1201 tccatcacct ccatctcgtc catcttctcg ggcggctcgg ggatgtcctt ggcgccgtgg
     1261 atctcgaacc cggtcttcgc gacgaccttc tcgaccaccg tcggatcggc cagcaccacc
     1321 acgacgtcgc ccgggaacag ccgccagtcc tcggacggga tgtagcgccg cccgccctcg
     1381 cgcacgatcg cggcgaccga catctcgccc tcgcccatct cctcgagccc gcgcacggtc
     1441 agtccgggcg acgccggttg cgccgggatg cgcagctcgg tcgtgtaggc ctcgatcgag
     1501 aacgcctcct cggcggactt gtggccttca cgttcgggca gcaggcgcca gccgaaggcg
     1561 aggaagacca ccgccagggt cgtcagcggc aaaccgacgg cgacgaagtc gaacagcccg
     1621 aacggcggcc gcccgagctc gtggcgcacg gtggagatca gcaggttcgg cgaggtcccg
     1681 atcttcgtga tcgtgccgcc gaccagcgag ccgaacgcga gcggcatcag gtacatcgag
     1741 cgcggccgcc ccgagcgccg ggccgtctgg atcgccagcg gcatcacgat ccccagcgtg
     1801 ccgacgttct tcacgaacgc cgacaggtag gcgaccgacg cggtcagcac gccgatctgc
     1861 aacgaggtcg acttcacccg cgacagcagc gagccgatcc agacgtccag cacgttggac
     1921 gccgcgatcg cgcggctgac caccagcacc gacgcgatga tcacgatcac cgggttggcg
     1981 aagccgagga aggccttgtc caccggcacg acgcccagca ccaccgccac caccagcgcg
     2041 agcccggcca cgatgtcgta ggagaggcgg ccgctcatga aggcggccag catcgcggcg
     2101 atgatcagga tggattcgat ctggtgcagc gtcatggagg ggcggtcggg cgccccgggg
     2161 cgggcgcgcc ggcggccgtg gcaaacggga tgccgcgagg cgcgcgccct cg
//
LOCUS       DASVLN010000258         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_282360, whole genome shotgun sequence.
ACCESSION   DASVLN010000258 DASVLN010000000
VERSION     DASVLN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282360"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF453_05895"
     CDS             <1..411
                     /locus_tag="VF453_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX7687215.1"
                     /translation="PPGVAAIDVRDAWARPTVPGQTSSVVYARITAPSGGRLVGASSP
                     VGDVSIHDMHMEGDIMRMRDLDAIDLPAGQAVALAPNGLHLMLTGLKRPLAAGAKVPV
                     TLSVVDREGRRGTLAVQVPVAASAPADEEGHHHG"
     gene            404..1453
                     /locus_tag="VF453_05900"
     CDS             404..1453
                     /locus_tag="VF453_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7687216.1"
                     /translation="MADLRRPLLAAALSAAAAPVLACATCGCSLSSDAAMGYTTETGW
                     RMSLQYDWIDQKQLRSGTHAISAHDVAAINDAGGDQEVEHDTVNRYTTLGLSWAPNAD
                     WGVRLLVPWVDRGHSTYGSSTNPLSSDDLSSAKIVGLGDVRVVGTWQGLLPGGNLGLQ
                     LGLKLPTGRYGGPDADGTGIAGRRPVAFRDGPLSREDSPGNLVDTSLQPGTGSTDVLL
                     GAFWHTAVTHEVDTFVNAQFQAAVSERLHQAGADFRPGNTTFVSVGARYEADPALIPQ
                     VQVNVSHKGADRGALADNPDSAGTVVYVSPGATWSPQPRVQLFAFAQIPVHSNLVGIQ
                     LAPRWTASGGISVGF"
     gene            complement(1496..>2122)
                     /gene="epsC"
                     /locus_tag="VF453_05905"
     CDS             complement(1496..>2122)
                     /gene="epsC"
                     /locus_tag="VF453_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEX7687217.1"
                     /translation="AGEDERAIDARALAIVRDFGAALPAIRELLDTDVLAAYRGDPAA
                     RSVDEVLLCYPGLHAVIHHRVAHQLYRLGAPLLARIVAELAHGDTGIDIHPGARIGSH
                     FFIDHGTGVVIGETAVIGRNVRIYQAVTLGAKNFAVDAEGALIKGGPRHPQVEDDVVI
                     YAGATVLGRVTIGRGASIGGNVWLTHDVPPGARITQATAHADRNGEAR"
BASE COUNT          269 a          801 c          777 g          275 t
ORIGIN      
        1 ccgcccggcg tcgccgcgat cgacgtgcgc gacgcctggg cccggcccac ggtgccgggg
       61 cagacgtcct cggtcgtcta cgcgcgcatc acggcgccct cgggcggccg gctggtcggc
      121 gcgagctcgc cggtcggcga cgtctcgatc cacgacatgc acatggaggg cgacatcatg
      181 cgcatgcgcg acctcgacgc gatcgacctg cccgccggcc aggccgtggc gctcgccccc
      241 aacggcctgc acctgatgct gaccggcctg aagcggccgc tggccgccgg cgcgaaggtg
      301 ccggtcacgc tgagcgtcgt cgaccgcgag ggccgccgcg gcacgctcgc cgtgcaggtg
      361 cccgtcgccg cctcggcgcc cgccgacgag gagggccatc atcatggctg acctgcgccg
      421 tccgctcctg gccgccgcgc tgtcggcggc cgccgccccc gtcctcgcct gcgcgacctg
      481 cggctgctcg ctgtccagcg acgccgcgat gggctacacc accgagaccg gctggcgcat
      541 gagcctgcaa tacgactgga tcgaccagaa gcagcttcgc agcggcaccc acgcgatctc
      601 cgcccacgac gtcgccgcga tcaacgatgc cggcggcgac caggaggtcg agcatgacac
      661 cgtcaaccgc tacaccacgc tgggcctgtc atgggcgccg aacgccgact ggggcgtgcg
      721 cctgctcgtg ccctgggtgg accgcgggca ctcgacctac ggctcgtcga cgaaccccct
      781 gagctctgac gacctgagca gcgcgaagat cgtcgggctc ggcgacgtgc gcgtcgtcgg
      841 cacctggcag ggcctgctgc cgggcggcaa cctcggcctc cagctggggc tgaagctgcc
      901 gaccggccgc tacggcgggc cggacgcgga cggcaccggc atcgccggcc gccgcccggt
      961 ggcgttccgc gacggcccgc tctcgcgcga ggattcgccg ggcaacctgg tcgacaccag
     1021 cctgcagccc ggcaccggct cgacggacgt gctcctcggc gcgttctggc acaccgccgt
     1081 gacgcacgag gtcgacacct tcgtcaacgc gcagttccag gcggcggtct ccgagcgcct
     1141 gcaccaggcc ggcgcggact tccgccccgg caacacgacc ttcgtgagcg tcggtgcgcg
     1201 ctacgaagcc gacccggcgc tgatcccgca ggtgcaggtc aacgtctcgc acaagggcgc
     1261 cgaccggggc gcgctggccg acaaccccga cagcgccggc accgtggtct acgtgagccc
     1321 gggcgcgacc tggtcgccgc agccgcgcgt gcagctgttc gcgttcgcgc agatccccgt
     1381 gcactcgaac ctggtgggca tccagctcgc gccgcggtgg acggcctcgg gtgggatctc
     1441 ggtgggcttc tgagtaacga cgcgccgggc gccccgcgag ggcgcgcccg gcgcgtcagc
     1501 gcgcctcgcc gttgcggtcg gcgtgcgcgg tcgcctgcgt gatgcgcgcg ccgggcggca
     1561 cgtcgtgcgt gagccagacg ttgccgccga tcgaggcgcc gcggccgatg gtcacgcggc
     1621 ccagcacggt cgcgccggcg tagatgacga cgtcgtcctc gacctgcgga tggcgcgggc
     1681 cgcccttgat cagcgcgcct tcggcgtcga ccgcgaagtt cttggcgccg agcgtgacgg
     1741 cctggtagat gcggacgttg cggccgatca ccgccgtctc gccgatcacg acgcccgtgc
     1801 cgtggtcgat gaagaagtgc gagccgatgc gcgcgcccgg gtggatgtcg atgccggtgt
     1861 cgccgtgcgc gagctcggcg acgatgcgcg cgagcagcgg cgccccgagc cggtacagct
     1921 ggtgcgcgac gcggtggtgg atcacggcgt gcaggccggg gtagcacagc agcacctcgt
     1981 cgacgctgcg tgccgccgga tcgccccggt aggcggcgag gacgtcggtg tcgagcagct
     2041 cgcggatcgc cggcagcgcg gcgccgaagt cgcgcacgat ggccagcgcg cgcgcgtcga
     2101 tggcgcgctc gtcctcgccg gc
//
LOCUS       DASVLN010000259         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_443916, whole genome shotgun sequence.
ACCESSION   DASVLN010000259 DASVLN010000000
VERSION     DASVLN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443916"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VF453_05910"
     CDS             complement(<1..938)
                     /locus_tag="VF453_05910"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX7687218.1"
                     /translation="MARPTRAAARIPRVPASSPEAPRARESSALTLTLVFAGLVCYAS
                     LYPFSGWFWPAGQPLSRLIVPPWPRYHVGFDDWSNFVGYMPLGALVYASALRRGRGQA
                     AAGALACVLPSALSWVMEFTQHFLPGRYPSLMDWVLNSLGACTGASLAIAAHASGAGR
                     RFMPLRERWFTPRSGFMQALLVLWPIGFLYPGTVPFGQGISWERVQEALYDLLSDHPL
                     AADFEWLWASLGENAAPMSQAAAGFAVAVGLAAPCLVAFSITRRGWRRAAMVLAAVGA
                     GLAANTLSASLNFGPVHALAWFSPAVPAGLALGALVA"
     gene            complement(965..1405)
                     /locus_tag="VF453_05915"
     CDS             complement(965..1405)
                     /locus_tag="VF453_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX7687219.1"
                     /translation="MSSGYLWVKAFHIVFVASWFAGLFYLPRIFVNLAQVPADSHAER
                     ERLLLMARKLYRFSSMLMVVAIAFGLALWLGYGIGIGPHSGWLHAKLLLVVLAIGYHH
                     RCRGLLRSFEKMENRRRERWFRVFNEMAILLFAAIVVLVVVKPF"
     gene            1720..2718
                     /gene="hemB"
                     /locus_tag="VF453_05920"
     CDS             1720..2718
                     /gene="hemB"
                     /locus_tag="VF453_05920"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX7687220.1"
                     /translation="MSHLPPFPTSRPRRLRRDAFSRELVREHRLSPGNLILPVFVVEG
                     GDVVQDIPSMPGVQRVGIERLLGVAQRCVTLGVPALALFPAIDPSLKTPDGAEALNPD
                     GLIPRAVRALKARFPELGVMTDVALDPYTSHGQDGLLDDSGYIVNDPTVQILVRQARV
                     QAEAGVDIVAPSDMMDGRIGAVRAMLEAQGLIHTRIMAYSAKYASAFYGPFRDAVGSA
                     AALGKSNKKVYQMDPGNSDEALREVSLDIAEGADMVMVKPGMPYLDIVRRVKDEFRMP
                     TFVYQVSGEYAMLKAAAANGWLDGPAVMMESLLAFRRAGADGVLTYFALEAAQLLR"
     gene            complement(2723..>3157)
                     /gene="ybeY"
                     /locus_tag="VF453_05925"
     CDS             complement(2723..>3157)
                     /gene="ybeY"
                     /locus_tag="VF453_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX7687221.1"
                     /translation="LQLSLQFADPSHREQLPRHRVARWIRAALEVPGTVTVRIVGEEE
                     GRALNRDYRGKDYATNVLTFDYVQEPEVEADLVLCAPVVEREAAAQGTSIADHYAHLL
                     VHGTLHAQGYDHEDDGPEAAAMEARETEILAGFGIADPYARA"
BASE COUNT          517 a         1126 c         1121 g          393 t
ORIGIN      
        1 gcgaccagcg cgccgagcgc cagccccgcg ggcaccgccg gcgagaacca cgccagcgca
       61 tgcaccggcc cgaagttgag cgaggccgac agcgtgttgg ccgcgagccc ggcgccgacc
      121 gcggccagca ccatcgcggc ccgccgccag ccgcggcggg tgatcgagaa cgcgaccagg
      181 cacggagcgg ccagcccgac cgccaccgcg aatcccgcgg ccgcctgcga catcggcgcc
      241 gcgttctcgc ccaggctcgc ccagagccac tcgaagtccg cggcaagcgg atggtcggag
      301 agcaggtcgt agagcgcctc ctgcacgcgc tcccacgaga taccctgccc gaacggcacc
      361 gtgcccggat agaggaagcc gatcggccac agcaccagga gtgcctgcat gaagccgctg
      421 cgcggggtga accagcgctc gcgcagcggc atgaagcgcc gcccggcgcc gctcgcgtgc
      481 gcggcgatgg ccaggcttgc gccggtgcag gcgcccagcg agttgagcac ccagtccatc
      541 agcgacggat agcgcccggg caggaagtgc tgggtgaact ccatgaccca cgacagcgcc
      601 gacggcagca cgcaggcgag cgcgccggcc gctgcctggc cgcgccctcg gcgcagcgcg
      661 ctcgcgtaga ccagcgcgcc cagcggcatg tagccgacga agttggacca gtcgtcgaat
      721 ccgacgtggt agcgcggcca gggcggcacg atcaaccggg acagcggctg ccccgccggc
      781 cagaaccagc cgctgaacgg atacaggctc gcgtagcaga ccagccccgc gaagaccagc
      841 gtcagcgtca gcgccgagct ctcgcgcgcg cgcggcgcct cgggcgacga ggcgggcacg
      901 cgcgggatgc gcgccgcggc gcgggtgggg cgggccatcg ggcggcggct gccggtcagc
      961 cgccctagaa cggcttgacg acgacgagca cgacgatcgc cgcgaacagc aggatcgcca
     1021 tctcgttgaa gacgcggaac cagcgctcgc ggcgacggtt ctccatcttc tcgaagctgc
     1081 gcaggagccc gcggcagcgg tggtggtagc cgatcgcgag gacgaccagg agcagcttgg
     1141 cgtgcagcca gccgctgtgc gggccgatgc cgatgccgta gcccagccac agcgccaggc
     1201 cgaacgcgat cgcgacgacc atcagcatgc tgctgaagcg gtagagcttg cgcgccatca
     1261 gcagcaggcg ctcgcgctcg gcgtggctgt cggcgggcac ctgcgcgagg ttcacgaaga
     1321 tccgcggcag gtagaacaag ccggcgaacc agcttgcgac gaagacgatg tggaaggcct
     1381 tgacccagag gtatccgctg ctcatggggc gccattatgg gcggcgaccc tgacgcgagg
     1441 cacgtgtccc gcgcgaggcc cggggcttgg cggtcgggct tgccggcgat cgcgggctcg
     1501 gaaaagaaag agccccggca tccacgaggg acggccgagg ctgcaaagcc ttgacgctgt
     1561 catcacgcaa ggcacctgct cagggaggaa aagcagggga caatgccctc acggacatca
     1621 tccgaaacgg aatgtagcag aactcccgac gagcgcgaca gtgctcgcga cccgtttcat
     1681 cgaagatccg tatttccgac gtaaaaggtt catcacactg tgtcgcatct gccacctttc
     1741 ccgaccagcc gcccgcgccg gctgcgccgc gacgcgttca gccgggagct cgtgcgcgag
     1801 caccggctga gtcccggcaa cctgatcctg cccgtcttcg tcgtcgaggg cggggacgtc
     1861 gtccaggaca tccccagcat gcccggcgtg cagcgcgtgg gcatcgagcg cctgctcggc
     1921 gtggcccagc gctgcgtcac gctgggcgtg cccgcgctcg cgctgttccc cgccatcgac
     1981 ccctcgctga agacgcccga cggcgccgag gcgctgaacc cggacggcct gatcccgcgc
     2041 gcggtgcggg cgctcaaggc ccgcttcccg gagctgggcg tgatgacgga cgtcgcgctg
     2101 gatccgtaca ccagccacgg ccaggacggc ctgctggacg acagcggcta catcgtcaac
     2161 gacccgaccg tgcagatcct ggttcgccag gcacgcgtgc aggccgaggc cggcgtcgac
     2221 atcgtcgccc cgagcgacat gatggacggc cgcatcggcg cagtgcgcgc catgctcgag
     2281 gcccaggggc tcatccacac ccggatcatg gcctacagcg cgaagtacgc gagcgccttc
     2341 tacggcccgt tccgcgacgc cgtcggctcg gcggcggcgc tgggcaagag caacaagaag
     2401 gtctaccaga tggatccggg caacagcgac gaggccctgc gcgaggtgag cctggacatc
     2461 gccgagggcg ccgacatggt catggtcaag cccggcatgc cgtacctgga catcgtccgg
     2521 cgggtgaagg acgagttccg catgcccacc ttcgtctacc aggtcagcgg cgagtacgcg
     2581 atgctcaagg ccgccgccgc caacggctgg ctggacggtc cggccgtgat gatggagtcg
     2641 ctgctggcct tccgccgcgc cggcgccgac ggcgtgctga cctacttcgc gctcgaggcg
     2701 gcgcagctgc tgcgctgacg gctcaggcgc gcgcgtaggg atcggcgatc ccgaagccgg
     2761 cgaggatctc ggtctcgcgc gcctccatgg ccgcggcctc ggggccgtcg tcctcgtgat
     2821 cgtagccctg cgcgtgcagc gtgccgtgca ccagcaggtg cgcgtagtgg tcggcgatcg
     2881 acgtgccctg cgccgcggcc tcgcgctcga ccaccggcgc gcacagcacc aggtcggcct
     2941 cgacctcggg ctcctgcacg tagtcgaagg tcagcacgtt ggtcgcgtag tccttgccgc
     3001 ggtagtcgcg gttcagcgcg cggccctcct cctcgcccac gatgcgcacg gtgaccgtgc
     3061 cgggcacctc gagcgcggcg cggatccagc gcgcgacgcg gtggcgcggc agctgctcgc
     3121 ggtgggaggg atcggcgaac tgcaggctca gctgcag
//
LOCUS       DASVLN010000260         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_120620, whole genome shotgun sequence.
ACCESSION   DASVLN010000260 DASVLN010000000
VERSION     DASVLN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120620"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..2288
                     /locus_tag="VF453_05930"
     CDS             36..2288
                     /locus_tag="VF453_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7687222.1"
                     /translation="MTLHRDASLEASDRRPTVLLVDDDEVNLLMTAAALRDRGFDITS
                     ATGGEEALTRLASWTPDVVVLDAIMPGLDGFETCVALRHMPGFDNVPVLMLTGLDDEA
                     SINRAYQVGATDFFVKATQWSLLAGRLRYLLRASRTRQELERSKAKLARAQDLARMGS
                     FDWWMGDGRSFVNGLELSEEGLRVFGYGPDDRVNLRDMLRMIAPTDREPLVRLARQIL
                     YQVSVIATDVPMTLPDGRFRIIHVEAEPEFDEHGHCCAYTGIVQDVTDRRIAEDRIRH
                     LANYDALTNLPNRRQLIWRAERALEFGRRLGHQVALLLIDLDRFKVINDTLGHAAGDE
                     LLMEVAFRLRGCVRHCEQLFEGVIETAGSRSHRTLEAVGRLGGDEFVALLPEVADEAD
                     AQRVASRILEALREPVVVAGQDCFATASVGIALFPRDGNTVADLLRNSDVAMYAVKSQ
                     GRNDALVYTPRLAGRGREKLALESALHKAIERNELVLYYQPKIDVVSGRMVGAEALMR
                     WRRGDLLIQPADFIPVAEETGLIVPMSEWALREAARQARIWADAFGFSHSIAVNMPNR
                     MFERTDLIEQITAAVREEGIPHSMIQLEITETGLMKDLQRVIPSLHRLTEIGVQIAVD
                     DFGTGYSSLAYLTSLPISEVKIDRSFVRDLGVLPQSLAVVTAIIALAKSLDLKVVAEG
                     VENQRQQDVLYGEGCTLMQGFLFSKPMPPEDVPSWLEQVVLPRKAPWVPGAAKARAGA
                     DDVIDVGRPS"
BASE COUNT          362 a          898 c          829 g          339 t
ORIGIN      
        1 gccgcatgcc gcgacccccg ccggggagcg cggcgatgac gctgcatcgc gacgccagcc
       61 tcgaggccag cgaccgtcgt ccgacggtgc tgctggtcga cgacgacgag gtcaacctgc
      121 tcatgaccgc cgccgcgctc cgcgaccgcg gattcgacat cacctcggcc accggcggcg
      181 aggaggccct cacgcggctg gcgagctgga cgccggacgt ggtcgtcctc gacgccatca
      241 tgccgggcct ggacggcttc gagacctgcg tcgcgctgcg ccacatgccg ggcttcgaca
      301 acgtgccggt gctgatgctc accggcctgg acgacgaggc ctcgatcaac cgcgcctacc
      361 aggtcggcgc gaccgacttc ttcgtcaagg cgacgcagtg gagcctgctc gccggccgcc
      421 tgcgctacct gctgcgcgcg tcgcgcacgc gccaggagct cgagcgcagc aaggccaagc
      481 tcgcgcgcgc ccaggacctc gcccgcatgg gcagcttcga ctggtggatg ggcgacggcc
      541 gaagcttcgt caacggcctg gagctgtccg aggaaggcct gcgcgtgttc ggctacgggc
      601 ccgacgaccg cgtcaacctg cgcgacatgc tgcgcatgat cgccccgacc gaccgcgagc
      661 cgctcgtgcg cctggcgcgc cagatcctgt accaggtctc cgtgatcgcc accgacgtgc
      721 cgatgacgct gccggacggg cgctttcgca tcatccacgt cgaggcggag ccggagttcg
      781 acgagcacgg ccactgctgc gcctacacgg gcatcgtgca ggacgtgacc gaccgccgca
      841 tcgcggaaga ccgcatccgc cacctcgcca actacgacgc cctgaccaac ctgcccaatc
      901 gccggcagct gatctggcgc gcggagcggg cgctcgagtt cggccgccgc ctcggccacc
      961 aggtggcgct gctgctgatc gacctcgacc gcttcaaggt gatcaacgac acgctgggcc
     1021 acgccgccgg cgacgagctg ctgatggagg tcgcgttccg cctgcgcggc tgcgtgcgcc
     1081 actgcgagca gctgttcgag ggcgtgatcg agaccgcggg ctcgcgctcg caccgcacgc
     1141 tggaggccgt gggccgcctg ggcggcgacg agttcgtcgc cctgctgccc gaggtcgccg
     1201 acgaagccga cgcgcagcgc gtcgcctcgc gcatcctgga ggcgctgcgc gaaccggtgg
     1261 tggtcgcggg ccaggactgc ttcgccaccg cgtcggtcgg catcgcgctg ttcccgcgcg
     1321 acggcaacac ggtggccgac ctgctgcgca actccgacgt cgccatgtac gcggtgaagt
     1381 cccagggccg caacgacgcg ctggtgtaca cgccgcgcct ggcgggccgc gggcgcgaga
     1441 agctggcgct cgagagcgcg ctgcacaagg cgatcgagcg caacgagctg gtgctgtact
     1501 accagcccaa gatcgacgtc gtgtcggggc gcatggtcgg cgcggaggcg ctgatgcgct
     1561 ggcgccgcgg cgacctgctg atccagccgg ccgacttcat ccccgtggcc gaggagaccg
     1621 gcctgatcgt cccgatgagc gagtgggcgc tgcgcgaggc cgcgcgccag gcgcgcatct
     1681 gggcggacgc gttcggcttc tcgcactcga tcgccgtcaa catgcccaac cgcatgttcg
     1741 agcgcaccga cctcatcgag cagatcacgg cggccgtgcg cgaggaaggc atcccgcaca
     1801 gcatgatcca gctggagatc accgagaccg gcctcatgaa ggacctgcag cgcgtgatcc
     1861 cgtcgctgca ccggctcacg gagatcggcg ttcagatcgc ggtcgacgac ttcggcaccg
     1921 gctattcctc gctcgcctac ctgacctcgc tgccgatctc ggaggtcaag atcgaccgca
     1981 gcttcgtgcg cgacctcggc gttcttccgc aaagcctggc agttgtcacg gcgatcatcg
     2041 cgctcgcgaa gtcgctggac ctaaaggtcg tggccgaagg tgtcgaaaac cagagacagc
     2101 aggatgttct ctacggcgag ggctgcacgc tgatgcaggg gttcctgttc agcaagccga
     2161 tgccgccgga ggacgtcccg tcctggctcg agcaggtcgt cctgccgcgc aaggcgccct
     2221 gggtgcccgg agccgccaag gcccgggccg gcgccgacga cgtcatcgac gtcggtcggc
     2281 cgtcctgagc gcctgcagcg cgggcgggcg gctgccgccg cccctttgcc ttcgccgtcg
     2341 tccatgcccg ccacccagcc caaacgcgag accccgcgcc ccgcggccgc cgccaagccc
     2401 gccgcacccg gcgccactcc caagccgg
//
LOCUS       DASVLN010000261         5338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_120642, whole genome shotgun sequence.
ACCESSION   DASVLN010000261 DASVLN010000000
VERSION     DASVLN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120642"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..986
                     /locus_tag="VF453_05935"
     CDS             150..986
                     /locus_tag="VF453_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX7687223.1"
                     /translation="MLLRHSAAADAAAPTPGGLPVDIRLMNAIAVVVLALVAIGLAGA
                     GARWLALRPAFSFEDLQLEGDLQRNSVTTVRANALPHLKGNFFTMDLGRARAAFEQVP
                     WVRRAVVRRVWPDRLVVTLEEHQPVALWSAGDDASDRMVNSYGEVFEANVGDVEEDGL
                     PEFSGPDGSAAQVLAMYRRLKPVFAQMDAEITALALSGRGSWRAELDSGAAIELGRGS
                     DDEVVERGMRFVRTLPQVLKRIGAPLESADLRHAEGYAVKFKGVGFGADPKQPPAAAR
                     RQ"
     gene            1007..2239
                     /gene="ftsA"
                     /locus_tag="VF453_05940"
     CDS             1007..2239
                     /gene="ftsA"
                     /locus_tag="VF453_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX7687224.1"
                     /translation="MAKEYKDLVVGLDIGTAKVMAVVAEMLPGGELRLAGLGVAPTHG
                     LKRGVVVNIDATVQSIQQALKEAEMMADCKISRVYTGITGSHIRGQNSTGMVIVRDNR
                     EVTPTDMARVVETAKAINIPNDQRVLLVEPQEFVIDGHEVKEPIGMSGSRLEVRVHIV
                     TGAQSAAENIVKCVRRCGLEVDQLVLNPSASSQAVLTQDEMDLGVALVDIGAGTTDVA
                     IFTGGSVRHTAVIPIAGDLITSDIAMALRTPTKDAEEIKVEYGVAKQLLADATEQVEV
                     PGLGDRAPRMLSRQALAGVIEPRVEEIFSLVHQVIRESGCEELLSSGIVLTGGTAVMP
                     GMVELGEDIFLKPVRKGLPTYSGALFDMVANPRSATVMGLLEEARIAHSRGHKAAAQA
                     GSMQTFFGKARDWFMGNF"
     gene            2388..3638
                     /gene="ftsZ"
                     /locus_tag="VF453_05945"
     CDS             2388..3638
                     /gene="ftsZ"
                     /locus_tag="VF453_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7687225.1"
                     /translation="MPIEMIEEFDQGTQIKVIGVGGGGGNAVEHMVSEGVQGVEFICA
                     NTDAQALNRSGASSIIQLGHKGLGAGGKPEVGKAAAEEAIEQIKAAIQGANMLFITAG
                     MGGGTGTGAAPVIARVAKEMGILTVGVVTKPFEFEGGRRMKSADAGVAELEANVDSLI
                     VVLNEKLLEVLGDDVTQDQAFAHANDVLKNAVGGISDIIHIPGLVNVDFEDVKTVMSE
                     PGKAMMGTAIAQGPDRATKAAEAAVACPLLEGIDLSGAKGVLVLIAASRQSFKLSESR
                     LAMNTIRRYAAEEAHVIYGTAYDETLGDQLRVTVIATGLSQSRRAQAPLTVVQTVQHA
                     RTGTDNLPLMGNPAAGHANAPMMGSVQQQPIAPAMPSNTDYGHLGTPSVWRHRQHAAA
                     KVEALSSHGMDEIEIPAFLRKQAD"
     gene            3707..4477
                     /locus_tag="VF453_05950"
     CDS             3707..4477
                     /locus_tag="VF453_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7687226.1"
                     /translation="MSRLLLLLFAGLKFSKLFLSVGSMLLAVGVYALVFGWRYAVGIV
                     AMLVIHEFGHFLAARQRGLKAELPMFIPFAFAWVRLRDRPHTAETEAFVALAGPLVGT
                     VGALGAYFLAHNENVPWLLAVAYTGFFINLFNLIPIPPFDGGRITQILSPRIWLLGLP
                     VLGLMMWYSFSPILVVIAILAVPYAIAAFKFRADTPEAQAYYALVSPRVRWEYAFYYV
                     VLMVFLVFMMHDTHQQLAGYGNGGDRVEQTEDSGAGQV"
     gene            complement(4548..>5338)
                     /locus_tag="VF453_05955"
     CDS             complement(4548..>5338)
                     /locus_tag="VF453_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687227.1"
                     /translation="APPPAAASARARAQGDMRPQVISTTDVDLTCNGGGTATITITNG
                     TDASEHNSVFDAGEVYDVTFNSCVAFTGLDALNGTIHMTIDSVANGTTSATMTATNLA
                     VAGGLATYNGTATIARTTAATASGTTQTSTIAATGLTLAGAYNGHNDTFTVGSLAGTR
                     TTTWDTSPAPTGWTWSGSDALSGTAGGTRTFATTTSTTTALAYGADGTPTAGAWTVTR
                     TDSNARITATIDGSSVDFTVDDSGDGTVDDSWTDTLANLDAAIG"
BASE COUNT          796 a         1786 c         1972 g          784 t
ORIGIN      
        1 cactcgctgg tgccgatcgc cgcgcgcggc gcgggcatca gctacgagcg cctgtgcacg
       61 atgctggtgg ccgcggcggc gctcgagccc gtcggccgcg gcggcaagcc cgcgaccaat
      121 gaccaggagg gcgcgcgcgc ctgaccgcca tgctgctgcg ccattccgcc gccgccgacg
      181 ccgccgcgcc caccccgggc gggctgccgg tggacatccg cctgatgaac gcgatcgccg
      241 tcgtggtgct cgcgctggtg gcgatcggcc tcgcgggcgc gggcgcacgc tggctcgcgc
      301 tgcggccggc cttctcgttc gaggacctcc agctcgaggg cgacctgcag cgcaacagcg
      361 tgaccacggt gcgtgccaac gcgctgccgc acctgaaggg caacttcttc acgatggacc
      421 tcggccgtgc gcgcgcggcc ttcgagcagg tgccctgggt gcgccgtgcc gtggtgcgcc
      481 gggtgtggcc ggaccggctc gtggtgacgc tggaggaaca ccagcccgtg gcgttgtggt
      541 cggccggcga cgacgcgagc gaccgcatgg tcaacagcta cggcgaggtc ttcgaggcca
      601 acgtcggcga cgtcgaggag gacggcctgc ccgagttctc cggcccggac ggcagcgccg
      661 cgcaggtgct cgcgatgtac cggcgcctga agccggtgtt cgcgcagatg gacgccgaga
      721 tcacggcgct cgcgctgtcg ggccgcggca gctggcgcgc ggagctcgac tcgggcgcgg
      781 cgatcgaact ggggcgcggc agcgacgacg aggtggtcga gcgcggcatg cgcttcgtgc
      841 gcacgctgcc gcaggtgctc aagcgcatcg gcgcgccgct ggaaagcgcc gacctgcgcc
      901 acgcggaagg ctacgccgtg aagttcaagg gcgtgggctt cggcgccgac cccaagcagc
      961 cgcccgccgc cgcgcgccgg caatgagaga gtgagctgag aaagatatgg ccaaggaata
     1021 caaggatctc gtcgtcgggc tcgacatcgg caccgccaag gtgatggccg tcgtcgccga
     1081 gatgctgccc gggggggaac tgcggctggc gggcctgggc gtcgcgccca cgcacggcct
     1141 gaagcgcggc gtggtcgtca acatcgacgc gacggtgcag tccatccagc aggccctgaa
     1201 ggaggccgag atgatggccg actgcaagat ctcgcgcgtc tacaccggga tcacgggcag
     1261 ccacatccgc ggccagaact ccactggcat ggtgatcgtg cgcgacaacc gcgaggtcac
     1321 gcccaccgac atggcgcgcg tggtggagac cgccaaggcg atcaacatcc ccaacgacca
     1381 gcgcgtgctg ctcgtcgagc cgcaggagtt cgtgatcgac ggccacgagg tcaaggagcc
     1441 gatcggcatg agcggcagcc gcctcgaggt gcgcgtgcac atcgtgacgg gggcgcagtc
     1501 ggcggcggag aacatcgtca agtgcgtgcg gcgctgcggc ctggaggtcg accagctggt
     1561 gctcaacccg agcgcgtcga gccaggcggt gctcacgcag gacgagatgg acctcggcgt
     1621 cgcgctggtg gacatcggcg ccggcaccac cgacgtcgcg atcttcaccg gcggctcggt
     1681 gcgccacacc gcggtgatcc cgatcgcggg cgacctcatc acgagcgaca tcgcgatggc
     1741 gctgcgcacg ccgacgaagg acgccgagga gatcaaggtc gaatacggcg tcgccaagca
     1801 gctgctcgcc gatgcgaccg agcaggtcga ggtgccgggc ctcggcgacc gggcaccccg
     1861 catgctctcg cgccaggcgc tcgccggcgt gatcgagccg cgcgtcgagg agatcttctc
     1921 gctcgtgcac caggtgatcc gcgagtcggg ctgcgaggaa ctgctgtcct cgggcatcgt
     1981 gctgaccggc ggcacggcgg tgatgccggg gatggtggag ctcggcgagg acatcttcct
     2041 caagcccgtg cgcaagggcc tgccgaccta ctcgggcgcg ctgttcgaca tggtcgccaa
     2101 cccgcgctcg gcgacggtga tgggattgct ggaggaggcg cgcatcgccc acagccgcgg
     2161 ccacaaggcg gcggcgcagg cgggctcgat gcagaccttc ttcggcaagg cgcgcgactg
     2221 gttcatgggg aatttctgat gaagaaggtg atgctggaca tgagaggcgt cacccgcata
     2281 aggcccggcg cgcacgacgc gaccgagtcg aggattggga acgagacggg tattcggatc
     2341 aacggatttc aggcaactgc aacagcattc aagcaaggag tgaaaacatg ccgatcgaga
     2401 tgattgaaga gttcgaccaa ggcacgcaga tcaaggtcat cggcgtgggc ggcggcggcg
     2461 gcaacgccgt ggagcacatg gtcagcgagg gcgtccaggg cgtggagttc atctgcgcca
     2521 acaccgacgc gcaggcgctg aaccggtcgg gcgcgagctc gatcatccag ctgggccaca
     2581 agggcctggg cgcgggcggc aagcccgagg tcggcaaggc cgcggcggaa gaggcgatcg
     2641 agcagatcaa ggccgcgatc cagggcgcca acatgctgtt catcacggcc ggcatgggcg
     2701 gcggcacggg cacgggcgcc gcgccggtga tcgcgcgcgt ggccaaggag atgggcatcc
     2761 tgaccgtcgg cgtcgtgacc aagccgttcg agttcgaggg cggccgccgc atgaagtcgg
     2821 ccgacgcggg cgtcgccgag ctcgaggcca acgtcgactc gctgatcgtg gtcctcaacg
     2881 agaagctgct ggaggtgctg ggcgacgacg tcacgcagga ccaggccttc gcgcacgcca
     2941 acgacgtgct gaagaacgcg gtcggcggca tcagcgacat catccacatc ccgggtctcg
     3001 tgaatgtcga cttcgaggac gtgaagacgg tcatgagcga gccgggcaag gcgatgatgg
     3061 gcaccgcgat cgcgcagggc ccggatcgcg cgaccaaggc cgccgaggcc gccgtcgcat
     3121 gcccgctgct ggaaggcatc gacctgtccg gcgccaaggg cgtgctggtg ctgatcgccg
     3181 cgtcgcgcca gagcttcaag ctctcggaga gccgcctggc gatgaacacg atccgccgct
     3241 acgccgccga ggaagcgcat gtgatctacg gcaccgccta cgacgagacg ctgggcgacc
     3301 agctgcgcgt cacggtgatc gcgacgggcc tgtcgcagtc gcgccgcgcg caggcgccgc
     3361 tgacggtcgt ccagacggtg cagcacgccc gcaccggcac ggacaacctg ccgctcatgg
     3421 gcaatcccgc ggcgggccac gccaacgcgc cgatgatggg ctcggtgcag cagcagccga
     3481 tcgcgcccgc gatgccgagc aacaccgact atggccacct cggcacgccc agcgtctggc
     3541 gccatcgcca gcacgccgcg gccaaggtcg aggccctgag ctcgcacggc atggacgaga
     3601 tcgagatccc cgcgttcctg cgcaagcagg ccgattgacg gccaggcggg ggcgggcgcc
     3661 agcgctcgcc cccggggcgg ggcgcgccat ccgctaggat cgtgggatgt cgcgtctcct
     3721 gctcctgctt ttcgccggcc tcaagttcag caagcttttc ctgagcgtcg gctcgatgct
     3781 gctcgccgtg ggcgtgtatg cgctcgtctt cggctggcgc tatgcggtgg gcatcgtcgc
     3841 gatgctcgtg atccatgagt tcggccactt cctcgccgcg cgccagcgcg ggctgaaggc
     3901 ggagctgccg atgttcatcc cgttcgcgtt cgcgtgggtg cgcctgcgcg atcgtccgca
     3961 caccgcggag accgaggcgt tcgtcgcgct cgcggggccg ctcgtgggca cggtgggcgc
     4021 gctcggcgcc tacttcctcg cccacaacga gaacgtcccg tggctgctcg cggtcgccta
     4081 cacgggcttc ttcatcaacc tgttcaacct gatcccgatc ccgccgttcg acggcggccg
     4141 catcacgcag atcctgtcgc cgcggatctg gctgctgggc ctgccggtcc tgggcctgat
     4201 gatgtggtac tcgttctcgc ccatcctggt cgtgatcgcg atcctcgccg tgccctacgc
     4261 gatcgccgcg ttcaagttcc gcgccgacac ccccgaggcg caggcctact acgcgctcgt
     4321 cagcccgcgg gtgcgctggg agtacgcctt ctactacgtc gtgctgatgg tgttcctcgt
     4381 cttcatgatg cacgacaccc accagcagct ggcgggctac ggcaacggcg gcgaccgcgt
     4441 cgagcagacc gaggattcgg gcgccggcca ggtctgaggc ggctgcaagc gacgacgccg
     4501 cggccccctc gcggaggacg cggcgccgcc ggcgggaacg cagtcgctca gccgatggcc
     4561 gcgtcgaggt tggccagcgt gtccgtccag ctgtcgtcga ccgtgccgtc gccgctgtcg
     4621 tcgacggtga agtcgaccga gctgccgtcg atcgtcgcgg tgatcctggc gttgctgtcc
     4681 gtgcgggtga ccgtccacgc gccggccgtg ggcgtgccgt ccgcgccgta ggccagcgcg
     4741 gtcgtcgtgc tggtcgtcgt cgcgaaggtg cgggtgccgc cggccgtgcc ggagagggcg
     4801 tcggagccgc tccaggtcca gccggtcggc gccggcgagg tgtcccaggt cgtcgtgcgg
     4861 gtgcccgcga ggctgccgac cgtgaacgtg tcgttgtggc cgttgtacgc gccggccagg
     4921 gtgaggccgg tcgccgcgat cgtggaggtc tgcgtcgttc cgctggccgt cgccgccgtg
     4981 gtccgcgcga tcgtggcggt gccgttgtag gtggccaggc cgccggccac ggccaggttg
     5041 gtggcggtca tcgtcgccga ggtcgtgccg ttggcgacgg agtcgatcgt catgtggatc
     5101 gtgccgttca gcgcgtcgag gccggtgaac gcgacacagg aattgaaggt gacgtcgtag
     5161 acctcgcccg cgtcgaagac ggagttgtgc tcgctcgcgt cggtgccgtt ggtgatggtg
     5221 atggtggcgg tgccgccgcc gttgcaggtg agatccacgt cggtggtcga gatcacctgc
     5281 ggccgcatgt cgccctgcgc ccgggcgcgc gcgctcgcgg cggcgggcgg gggcgcgg
//
LOCUS       DASVLN010000262         9298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_335865, whole genome shotgun sequence.
ACCESSION   DASVLN010000262 DASVLN010000000
VERSION     DASVLN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335865"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="VF453_05960"
     CDS             <1..841
                     /locus_tag="VF453_05960"
                     /inference="COORDINATES: protein motif:HMM:NF021437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HEX7687228.1"
                     /translation="DQPLTAEAMTRAMTLAEHVQRALDASGAQVVIVARAGQDLRKYG
                     LQYSHLAFAYRESIPAPAPPPAAIPGTVSPMAAMGDPAAIAIDAARAAVPASPAPPAG
                     SRTVWRIAHKLNQCGTAHASVFRQGLGEFFLDTPFEYRAAFVVPEPEVQAALLPILAD
                     DARLVQWNTPAYSVVAYPWATTYQQSNQWALETLAGALEPAATTRARAQAWLQLHDYR
                     PTVLHLDAFTRLGARMTRANVAFDDHPNDQRFSDRIATVTVESMFDWLQRERLSGSPQ
                     TVY"
     gene            940..2862
                     /locus_tag="VF453_05965"
     CDS             940..2862
                     /locus_tag="VF453_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7687229.1"
                     /translation="MSTRPDTPPHAPPSQFALLGQRRFAPFFWTQFFGAGNDNVLKFA
                     FTVLVTYQLDVSWLPPEKAGLVISAVFILPYVLLSATAGQLADKYDKATMMRLIKSFE
                     IAIMLLAAWGFVGGQVVVLLLCLFLMGVHSTLFGPVKYAYLPQHLDDHEITGGNGMVE
                     MGTFVAILLGQLAGGLLVVVPGMGPALAAGACLALAVIGRLTAQAIPATPAEDPALKV
                     NWNPLSETLRNLRLAGKDIVVFRSLLGISWMWFFGATFLSQFPSFAKDVLHGDAHVAS
                     LLLVLFSVGVGIGSLLCEVLSRRQVEIGLVPLGAIGMSLFAIDLYFAVGHLAMPVGAG
                     VWGIAGFVGQGQHVRPMIDLFMLSLSAGLYSVPMYALIQTRAQPSHRARIIAANNILN
                     ALFMIASSLIAGALLHFGVSIPGVFLWTGIANAIVAGYIFLLVPEYLLRFFAFVLTRT
                     VYRFRIRGEEHIPLEGPAIVACNHVSFIDPVLLMAASPRPIRFLMDSRIFRTPGLGLL
                     FRLAKAIPIAPKSDDPAAYEAAFAAADQVLAEGDLLGIFPEGGITRDGQLMPFKGGIM
                     RILEKRPVPVVPLALQNVWGSFFSRIEGAAMTKPFRRGLFSHVGLHVGPRIAPAEVTP
                     EGLQREVGTLMETRVY"
     gene            complement(2880..3392)
                     /locus_tag="VF453_05970"
     CDS             complement(2880..3392)
                     /locus_tag="VF453_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687230.1"
                     /translation="MQPPRSSSWRALSTLGAWLVALVIVFEEWGWAPLARRMSALARL
                     PAVAWVERRIAALPPHAALVVFCVPMLLLLPVKVGALWLIAQGRASLGLLLFFGAKLL
                     STGVVARVFILTQPRLMALAWFARGYARWLAWKARVLARVRATAPWRAMRRMARRVRA
                     MQLRVKRRGR"
     gene            3501..4397
                     /locus_tag="VF453_05975"
     CDS             3501..4397
                     /locus_tag="VF453_05975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7687231.1"
                     /translation="MSVKNTRHVISTPRARAAMSLALLAVLAACGGNGDATPTATISQ
                     TTVTATRFGGTALVTIQGTGLDSTLVVSSQNCSGMTMLNTAPYVSSTTTAYYQCTVDG
                     GIQGNVVASSNGVVVGQGGFTVPQPIVTMAVGNGLAVSGNIVFSLRADVAPKTVANFL
                     HYVNTGFYNNTIFHRVVPGFVVQGGGYGPTVGGHLPAAKTTDTSIGLESSGLSNVQWS
                     IAMANDGGSPPMANSQFFVNLADNTGLDGGYTVFGTVSAGTDVVTAITNAPATCTANP
                     AASTTDCLPQPDVLVSSATQTQ"
     gene            complement(4435..5562)
                     /gene="tgt"
                     /locus_tag="VF453_05980"
     CDS             complement(4435..5562)
                     /gene="tgt"
                     /locus_tag="VF453_05980"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7687232.1"
                     /translation="MLTFELLRTDAHARRGRLTLNHGVVETPVFMPVGTYGTVKGVLP
                     RSLEEMGAQIILGNTFHLWMRPGLDVMKQFGGLHRFESWGRPILTDSGGFQVWSLGEM
                     RKISEEGVRFASPVNGDKLFLTPEGSMQIQTILNSDIVMQFDECTPYETKGVLTTERE
                     ARASMELSLRWAARCRDEFARLANPNALFGIVQGGMYEHLREASLDKLVALDFPGYAV
                     GGVSVGEPKEDMRRIVAHTPHRLPAHKPRYLMGVGTPEDLVYGVSQGVDMFDCVMPTR
                     NARNGHLFTRFGDLRLRNARYKSDQAPVDETCTCHTCANFSRAYLHHLDRCGEMLGPM
                     LTTIHNLHYYLNLMREVRGALDEGRFGEFQARFAADRARGV"
     gene            complement(5706..6041)
                     /locus_tag="VF453_05985"
     CDS             complement(5706..6041)
                     /locus_tag="VF453_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687233.1"
                     /translation="MQVRSLSAIAAVAAALFAGAVQAQEIDRGETLQARNLASQGADV
                     QQRSRVSVVAETRNLQAEGALKAVGEKADAPVVPAVPTVARGLTRAEVKADLAQWRSA
                     HPQVVGERG"
     gene            6223..7167
                     /locus_tag="VF453_05990"
     CDS             6223..7167
                     /locus_tag="VF453_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7687234.1"
                     /translation="MDQLRAMRVFARVIDTGGFAKAAQALDIAPAVATRLVAELEEHL
                     GARLINRTTRRLALTDVGEGYLERVRNILTEVDDAEAAANQATAEPRGRLSVLAPPGF
                     TVHQLAKHLPRFRKLHPKVSIDLTVAPSVEAPDENHDVTIIVQRQRPIDGAFIARRIA
                     MAEVILCATSEYLDRRGRPQHPSELADHEMLMPLPPSEQRELSFEPAWGGGAAVAVEL
                     SPTPPLTTTHVDTLYAAALAGMGIAGLPSFIAEDALMEVALERILPQWRLRALTLFAA
                     MPTRKHVPVRTRAFVDFLVETFASQRDPWLAAAGCESC"
     gene            complement(7201..8310)
                     /gene="queA"
                     /locus_tag="VF453_05995"
     CDS             complement(7201..8310)
                     /gene="queA"
                     /locus_tag="VF453_05995"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX7687235.1"
                     /translation="MTRDAVFLPPPSGEHVLADFDFELPPELIAQHPAAERSASRLLD
                     GTGAEPVDRVFRDLPALLEPGDLLVFNDTRVIKARLHGAKATGGGVEVLVERVLPPGE
                     AWAHIRASKSPRAGSTMRLGGEDGFDVEVLGRCGPDDALFHLRFPGDVFALLDRHGHV
                     PLPPYIEHADSEDDVRRYQTVFADRPGAVAAPTAALHFDDALLARIAERGVQSARVTL
                     HVGAGTFQPVRSESLVEHKMHTEWFDVPAATVEAVRRTRAAGGRVVAVGTTTVRALES
                     AATAAAQAGDPGGLRAGAGETRIFITPGYRFRAVDRLITNFHLPRSTLMMLVSAFSGM
                     EPIRALYAHAIARRYRFFSYGDAMLLARRDDAARG"
     gene            complement(8339..8488)
                     /locus_tag="VF453_06000"
     CDS             complement(8339..8488)
                     /locus_tag="VF453_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687236.1"
                     /translation="MRLVARFVVLLVAVCGVSVAFAWAWDRSDASHGAAATPDQADQA
                     GHAAS"
     gene            8650..>9298
                     /locus_tag="VF453_06005"
     CDS             8650..>9298
                     /locus_tag="VF453_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX7687237.1"
                     /translation="MPPSKQSPLPASAAPAPAARAELSGPQRALLRLGLVRPIDLALH
                     LPLRYEDETTLTPLRAAIPGEKVQVEGVVTDARVEARGRRQLVVRMADDEGTELVLRF
                     LNFYPSHQKTLAAGRRVRVRGELRGGFLGREMVHPTFKAVDGDVPLASSLTPVYPTTA
                     QLPQPYLRKAIAASLARAPLAEVLPPQVVPPGLPSLREALETLHHPPADLPLSALE"
BASE COUNT         1340 a         3384 c         3237 g         1337 t
ORIGIN      
        1 cgaccagccg ctcacggccg aggcgatgac gcgcgcgatg acgctggcgg agcacgtcca
       61 gcgcgcgctc gacgcgagcg gggcgcaggt ggtcatcgtc gcgcgcgcag gccaggacct
      121 gcgcaagtac ggcctgcagt actcgcacct ggccttcgcc tatcgcgagt cgattccagc
      181 gcccgcgccg ccgccggcgg cgatacccgg taccgtatcg ccaatggccg cgatgggcga
      241 ccccgccgcc atcgccatcg atgccgcccg ggccgccgtt ccggcgtcgc ccgcgccgcc
      301 tgcgggctcc cgcacggtct ggcgcatcgc gcacaagctc aaccagtgcg gcaccgcgca
      361 cgcctcggtg ttccgccagg gcctgggcga gttcttcctc gacacgccgt tcgaataccg
      421 cgccgccttc gtcgtccccg agcccgaggt ccaggccgcg ctgctgccga tcctggcgga
      481 cgacgcgcga ctcgtgcagt ggaatacgcc cgcctacagc gtcgtcgcct atccgtgggc
      541 gacgacctac cagcagagca accagtgggc gctcgagacg ctggccggcg cgctggagcc
      601 tgccgccacg acgcgcgccc gcgcccaggc ctggctgcag ctgcacgact accggcccac
      661 cgtgctgcac ctggacgcgt tcacgcgcct gggcgcgcgc atgacgcgtg cgaacgtcgc
      721 cttcgacgac catcccaacg accagcgctt cagcgaccgc atcgccacgg tgaccgtcga
      781 gtcgatgttc gactggctgc aacgcgagcg cctgtccgga tcgccgcaga ccgtgtattg
      841 acgccacccg cgccgaaaga cgagaaaccc atccatcgga cgcgctcgat cgccggctcc
      901 agcggctaca gttcagcgac ccatcccccg acgtccgcca tgagcacccg ccccgacacg
      961 ccgccgcacg cccctcccag ccagttcgcg ctcctcggcc agcgccggtt cgcgccgttc
     1021 ttctggacgc agttcttcgg cgcaggcaac gacaacgtgc tgaagttcgc gttcacggtg
     1081 ctggtcacct accagctcga cgtgagctgg ctgcccccgg agaaggccgg cctcgtgatc
     1141 agcgccgtct tcatcctgcc gtacgtgctg ctgtcggcca ccgccggcca gctggcggac
     1201 aagtacgaca aggcgaccat gatgcggctg atcaagtcgt tcgagatcgc catcatgctg
     1261 ctggcggcat ggggcttcgt cggcggccag gtcgtggtgc tgctgctgtg cctgttcctg
     1321 atgggcgtcc actcgacgct gttcggaccg gtgaagtacg cctacctgcc gcagcatctc
     1381 gacgaccacg agatcaccgg cggcaacggc atggtggaga tgggcacctt cgtcgccatc
     1441 ctgctggggc agctggccgg cgggctgctg gtcgtcgtgc cggggatggg ccctgcgctc
     1501 gcggccggcg cctgcctcgc gctcgcggtg atcggccgcc tcacggcgca ggcgatcccg
     1561 gccacgcccg cggaggatcc tgcgctgaag gtcaactgga acccgctgtc cgagaccctt
     1621 cgcaacctgc gcctcgcggg caaggacatc gtcgtcttcc gctcgctgct gggcatctcg
     1681 tggatgtggt tcttcggcgc gaccttcctc tcgcagttcc cgtccttcgc gaaggacgtg
     1741 ctgcatggcg acgcccatgt cgcctcgctg ctgctggtgc tgttctccgt gggcgtgggc
     1801 atcggctcgc tgctgtgcga ggtgctgtcg cggcgacagg tggagatcgg cctcgtgccc
     1861 ctgggcgcga tcggcatgag cctgttcgcg atcgacctgt acttcgccgt cggccacctg
     1921 gcgatgcccg tgggcgcggg cgtctggggc atcgccggct tcgtggggca gggccagcac
     1981 gtgcggccga tgatcgacct gttcatgctc tcgctgagcg ccggcctgta cagcgtgccg
     2041 atgtacgccc tgatccagac gcgcgcacag cccagccatc gcgcgcgcat catcgcggcc
     2101 aacaacatcc tgaacgcgct gttcatgatc gcgagctcgc tgatcgcggg ggcgctgctg
     2161 cacttcgggg tcagcatccc cggcgtgttc ctgtggaccg gcatcgccaa cgccatcgtc
     2221 gcgggctaca tcttcctgct cgtgccggag tacctgctgc gcttcttcgc cttcgtgctc
     2281 acgcgcacgg tctatcgctt ccgcatccgc ggcgaggagc acatcccgct cgagggcccg
     2341 gcgatcgtcg cgtgcaacca cgtgagcttc atcgatccgg tgctgctgat ggcggcaagc
     2401 ccgcgcccga tccgcttcct gatggactcg cgcatcttcc gcacgccggg actgggcctg
     2461 ctgttccggc tcgccaaggc gatcccgatc gcgcccaaga gcgacgaccc cgccgcctac
     2521 gaggccgcct tcgcagccgc cgaccaggtg ctggcggagg gcgacctgct gggcatcttc
     2581 cccgaaggcg gcatcacgcg cgacggccag ctgatgccgt tcaagggcgg catcatgcgc
     2641 atcctcgaga agcgcccggt gcccgtggtg ccgctggcgc tgcagaacgt ctggggctcg
     2701 ttcttctcgc gcatcgaggg cgcggcgatg accaagccct tccggcgcgg cctgttcagc
     2761 catgtcggcc tgcacgtcgg cccgcgcatc gcgccggcgg aggtcacgcc cgagggcctg
     2821 cagcgcgagg tcgggacgct catggagacg cgggtctact gagttgcgaa gcgcgtcggt
     2881 caacgtcccc gccgcttcac gcgcagctgc atcgcccgca ctcgccgcgc catcctgcgc
     2941 atcgcgcgcc atggcgcggt cgcacgcacg cgcgcgagga cgcgcgcctt ccacgcgagc
     3001 cagcgggcat aaccgcgcgc gaaccacgcc agcgccatca accgtggctg cgtgaggatg
     3061 aacacgcgcg ccacgacgcc ggtgctgagc agcttggcgc cgaagaacag cagcagcccg
     3121 aggctggcgc ggccctgcgc gatcagccac aacgcgccga ccttcaccgg cagcagcagc
     3181 agcatcggca cgcaaaagac gacgagggcg gcgtgcggcg gaagcgccgc gatgcggcgt
     3241 tcgacccagg ccacggcggg cagccgcgca agcgcactca tgcgacgcgc cagcggcgcc
     3301 cacccccatt cctcgaagac gatgacgagc gccaccagcc acgcccccag cgtcgagagc
     3361 gcgcgccagg acgacgaccg cggcggttgc acccgtgcga gcttaaactt agccggtttg
     3421 cgacccaaat taactccacg ttaacgcgac ggcggcgact gtcgacttag gatgtcgccc
     3481 tcattgcttc cgagacaagc atgtccgtca agaacactcg acacgtcatt tccacgccgc
     3541 gcgcgcgcgc cgcgatgtcg ctggccctgc tcgccgtcct cgcggcctgc ggcggcaacg
     3601 gcgacgccac ccccaccgcg acgatcagcc agaccaccgt cacggcgacc aggttcggcg
     3661 gcacggcgct cgtcacgatc cagggcaccg gcctggacag cacgctggtc gtgagctcgc
     3721 agaactgctc cggcatgacg atgctgaaca cggcaccgta cgtgagctcg acgacgacgg
     3781 cttactacca gtgcaccgtc gacgggggca tccagggcaa cgtggtcgcg tcttccaacg
     3841 gcgtggtggt cggccagggc ggcttcacgg tgccgcagcc catcgtcacg atggcggtgg
     3901 gcaacggcct ggcggtgagc ggcaacatcg tcttcagcct gcgcgccgac gtcgcaccca
     3961 agacggtcgc gaacttcctg cactacgtga acaccggctt ctacaacaac acgatcttcc
     4021 accgggtcgt gccgggcttc gtcgtgcagg gcggcggcta tggcccgacg gtcggcggcc
     4081 acctgccggc cgccaagacg accgacacct ccatcggcct ggagagctcc ggcctgagca
     4141 acgtgcagtg gtcgatcgcg atggccaacg acggcggctc gccgccgatg gccaactcgc
     4201 agttcttcgt caacctcgcg gacaacacgg ggctggacgg cggctacacc gtcttcggca
     4261 ccgtctccgc cgggaccgat gtcgtcaccg cgatcaccaa cgcgccggca acctgcaccg
     4321 cgaaccccgc ggccagcacg accgactgcc tgccgcagcc cgacgtgctg gtcagctcgg
     4381 cgacgcagac gcagtaagcg cgccgcggcc cggcgcgcca cgcgcgcggg gccgctacac
     4441 gcctcgagcg cggtcggccg cgaagcgcgc ctggaactcg ccgaagcgcc cctcgtccag
     4501 ggcgccgcgc acctcgcgca tcaggttcag gtagtagtgc aggttgtgga tggtggtcag
     4561 catcgggccc agcatctcgc cgcagcggtc gaggtggtgc aggtaggcgc ggctgaagtt
     4621 ggcgcaggtg tggcaggtgc aggtctcgtc gaccggcgcc tggtcggact tgtagcgcgc
     4681 attgcgcagg cgcaggtcgc cgaagcgcgt gaacaggtgg ccgttgcgcg cgttgcgcgt
     4741 cggcatgacg cagtcgaaca tgtccacgcc ctgcgagacg ccatagacga gatcctcggg
     4801 cgtgccgacg cccatcaggt agcgcggctt gtgcgccggc aggcgatgcg gcgtgtgggc
     4861 gacgatgcgg cgcatgtcct ccttgggctc gcccaccgag acgccgccca ccgcgtagcc
     4921 ggggaaatcc agcgccacca gcttgtcgag cgacgcctcg cgcaggtgct cgtacatgcc
     4981 gccctggacg atgccgaaca gcgcgttcgg gttggccagg cgcgcgaact cgtcgcggca
     5041 gcgcgccgcc cagcgcagtg acagctccat cgacgcccgc gcctcgcgct cggtggtcag
     5101 cacgcccttg gtctcgtagg gcgtgcactc gtcgaactgc atgacgatgt ccgagttcag
     5161 gatcgtctgg atctgcatgc tgccctcggg cgtgaggaac agcttgtcgc cgttgacggg
     5221 cgaggcgaag cgcacgcctt cctcggagat cttgcgcatc tcgcccagcg accagacctg
     5281 gaagccgccc gagtccgtga ggatgggcct gccccagctc tcgaagcggt gcaggccgcc
     5341 gaactgcttc atcacgtcca gcccgggacg catccagaga tggaaggtgt tgccgaggat
     5401 gatctgggcg cccatctcct cgagcgagcg cggcagcacg cccttgaccg tgccgtaggt
     5461 gcctaccggc atgaagaccg gggtctccac gacgccatgg ttcagcgtca ggcggccgcg
     5521 gcgggcgtgc gcgtcggtgc gcagcagttc gaatgtcagc atcggcatat tgtcgccggc
     5581 cgcgaaccgg gctcgcggcg ggccgcaagc ggcgacgccc cggcttgcgg ggcgtcgaag
     5641 gaaggacagg cccggccgat gcgggacggg cctcggggcc cggccgacgc cggccccggg
     5701 ggcgatcagc cgcgctcgcc gacgacctgc ggatgcgccg agcgccactg cgccaggtcg
     5761 gccttcacct cggcgcgcgt caggccgcgg gcgacggtcg ggaccgccgg cacgacaggc
     5821 gcgtcggcct tctcgccgac ggccttcagc gcaccttcgg cctgcaggtt gcgggtctcg
     5881 gcgacgaccg agacgcggct gcgctgctgc acgtcggcgc cttgcgacgc gaggttgcgc
     5941 gcctggaggg tctcgccacg gtcgatttcc tgggcctgga cggcaccggc gaacagggcg
     6001 gcggcaacgg cggcgatggc ggagagggag cggacttgca tgatgtattt cctgattcca
     6061 cggggttgag gcggtccgag cggtcgcagg tcgcgtcctg ccgggcctcg gtgatgcgga
     6121 ggtggtgtca gcgcgtcatc gatggcttca ctgtattccc cggaaaattc cggataaata
     6181 ggcaactata gaattgttta ttcccgatcc aggaagaatc cgatggacca gcttcgcgcc
     6241 atgcgggtgt tcgcccgcgt catcgacacc ggcggcttcg ccaaggcggc ccaggcgctg
     6301 gacatcgcac cggccgtcgc cacgcgcctg gtggccgagc tcgaggagca cctcggcgca
     6361 cgcctgatca accgaacgac gcggcgcctg gcgctcaccg acgtgggcga gggctacctg
     6421 gagcgcgtgc gcaacatcct caccgaggtc gacgacgccg aggccgccgc caaccaggcg
     6481 accgccgagc cgcgcggccg cctgagcgtg ctcgcgccgc cgggcttcac ggtgcaccag
     6541 ctcgccaagc acctgccgcg ctttcgcaag ctgcatccca aggtgtcgat cgacctgacc
     6601 gtcgcgccct cggtggaggc gcccgacgag aaccatgacg tcaccatcat cgtccagcgc
     6661 cagcgcccga tcgacggtgc gttcatcgcg cgccgcatcg cgatggccga ggtgatcctg
     6721 tgcgcgacct ccgagtacct cgaccgccgc ggccgtccgc agcatccctc ggaactcgcc
     6781 gaccacgaga tgctgatgcc gctgcccccg agcgagcagc gcgagctcag cttcgagccg
     6841 gcctggggcg gcggagcggc ggtcgcggtg gagctgagcc ccacgccgcc cctgaccacc
     6901 acccatgtcg acacgctcta cgccgcggcg ctcgccggca tgggcatcgc cggcctgccg
     6961 tccttcatcg ccgaggacgc gctgatggag gtcgccctcg aacgcatcct gccgcaatgg
     7021 cgcctgcgcg cgctgacgct gttcgccgcg atgcccacgc gcaagcacgt gcccgtgcgc
     7081 acgcgcgcgt tcgtggactt cctcgtcgag accttcgcga gccagcgcga tccgtggctg
     7141 gcggcggcgg ggtgcgagag ctgctaggcg cgggcagcgc agcgcgccgg ctcgcgacgc
     7201 tcaaccgcgg gcagcgtcgt cccgcctcgc cagcagcatc gcgtccccgt agctgaagaa
     7261 ccggtaccgc cgcgcgatcg cgtgcgcgta gagcgcgcgg atcggctcca tgccggagaa
     7321 cgcgctcacc agcatcatca gcgtgctgcg gggcaggtgg aagttggtga tcaggcggtc
     7381 gactgcgcgg aaccggtagc ccggcgtgat gaagatgcgc gtctcgcccg cgccggcgcg
     7441 caggccgccg ggatcgcccg cctgcgccgc ggcggtggcg gcggattcga gcgcccgcac
     7501 cgtcgtcgtg ccgaccgcga ccacgcggcc gcccgcggca cgcgtgcgcc gcacggcctc
     7561 gacggtggcg gccgggacgt cgaaccactc ggtgtgcatc ttgtgctcga cgaggctttc
     7621 gcttcgcacc ggctggaacg tgcccgcgcc gacgtgcagg gtcacgcgcg cgctctggac
     7681 gccgcgctcg gcgatgcggg cgagcagcgc gtcgtcgaag tgcagcgcgg ccgtcggtgc
     7741 ggcgaccgcg ccgggacggt cggcgaagac ggtctgatag cggcgcacgt cgtcctcgct
     7801 gtcggcgtgc tcgatgtagg gcggcagcgg cacgtggccg tggcgatcga gcagcgcgaa
     7861 gacgtcgccg gggaagcgca ggtggaacag cgcgtcgtcg gggccgcagc ggccgagcac
     7921 ctcgacgtcg aagccgtcct cgccgccgag ccgcatcgtg ctgccggcgc gcggcgactt
     7981 gctcgcgcgg atgtgcgccc acgcctcgcc cggcggaagg acgcgttcga ccagcacctc
     8041 gacgccgccg cccgtcgcct tcgcgccatg caggcgcgcc ttgatgacgc gggtgtcgtt
     8101 gaagacgagc aggtcgcccg gctcgagcag cgcgggcagg tcgcggaaga cgcggtcgac
     8161 gggctccgca ccggtgccgt ccagcaggcg cgacgcgctg cgctcggcgg cgggatgctg
     8221 ggcgatcagc tcgggcggga gctcgaagtc gaaatccgcg aggacgtgct cgccggacgg
     8281 cggcggcagg aagaccgcat cgcgggtcat cgcgacgtgt gcgtgcgcgc ggcgccgttc
     8341 aggaggccgc gtggccggcc tggtcggcct ggtcgggggt ggcggccgcg ccgtgcgacg
     8401 cgtcggaccg gtcccaggcc caggcgaagg cgacactcac gccgcagacg gcaaccagga
     8461 ggacgacgaa acgggcaacg agacgcatga tgtggcggtg ggatccgcgc ggtccgggcg
     8521 cgggcaggga tggacgcccg gtattgtcgt tgaaggcgcg cgccggcgac gtatccgctt
     8581 gtacccggag tgcactttcg tcgcattccg cgtcgggcgc gcaacgcggc tggggcgaga
     8641 atcgccgcca tgccgccctc caagcaatcg ccgctcccag cctccgccgc gccggcgccg
     8701 gccgcgcgtg ccgagctgag cggcccgcag cgcgcgctgc tgcggctggg gctggtgcgg
     8761 cccatcgacc tcgcgctgca cctgccgctg cgctacgagg acgagacgac gctcacgccg
     8821 ctgcgcgccg cgatccccgg cgagaaggtg caggtcgagg gcgtggtgac ggacgcacgc
     8881 gtcgaggcgc gcggccggcg ccagctcgtc gtgcggatgg ccgacgacga gggcaccgag
     8941 ctcgtgctgc gcttcctcaa cttctatccc tcgcaccaga agaccctcgc cgccggccgg
     9001 cgcgtgcgcg tgcgcggcga gctgcgcggc ggcttcctcg ggcgcgagat ggtgcatccg
     9061 acgttcaagg ccgtggacgg cgacgtgccc ctcgcgtcgt cgctgacgcc ggtctacccg
     9121 accaccgcgc agctgcccca gccctacctg cgcaaggcga tcgcggcgag cctcgcgcgc
     9181 gccccgctcg ccgaggtgct gccgccgcag gtcgtgccgc cgggcctgcc gtcgctgcgc
     9241 gaggcgctcg agacgctgca ccacccaccg gccgacctgc cgctgtcggc gctggagg
//
LOCUS       DASVLN010000263         1697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_175074, whole genome shotgun sequence.
ACCESSION   DASVLN010000263 DASVLN010000000
VERSION     DASVLN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175074"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /gene="sctT"
                     /locus_tag="VF453_06010"
     CDS             <1..814
                     /gene="sctT"
                     /locus_tag="VF453_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HEX7687238.1"
                     /translation="GPRSARAAGEPVDTIELAALQAWFTPVALTLPRLLAVFAIAPFF
                     SGNMMSGLVRNGVILMLAIFMSPAASDLPVLTLGGWVLIAAKEAFIGVLLGLGFGLFV
                     WAVQSVGDLVDFQTGSASAPFFDPVGGHEGGPTGQFLGWVVITLFMSAGGMLAMVRVI
                     VDSFRLWPVASFYPHMGEVIEQFAVRQGDTLFLWTVKLAAPIIFVLLLVELGIGLVGR
                     VAPQLNVFVFSQPVKSVLANLMLLLFLYFLYDSLQEFLKPENGVLDFLRNTL"
     gene            811..>1697
                     /locus_tag="VF453_06015"
     CDS             811..>1697
                     /locus_tag="VF453_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEX7687239.1"
                     /translation="MSANKDQKPTARRLREARKRGDVAFSSDIASAAVFTVVVGGLWL
                     LGATSFGLLQQLWLHATSAELLTRPDDRFPELLRHTGHVLLWIALPVTGLAALAGIAS
                     SFFQVGGILAFERLKPDVARLAPSRGLQRMFSTRNLINLVKMLVKTLLLIVLVGATVR
                     GLLDAALKLGYLRPDAILAAGAHALLGLFGAAAVIYAVLAGVDYAHQHYEYIKSLRMS
                     IDEVRRDYKEAEGDPLTRARRRGAHFEAIYAGLGDRVAASSAVLHSGRVAVALQYLGE
                     RDLPRVIARGENEVAMRLR"
BASE COUNT          199 a          587 c          607 g          304 t
ORIGIN      
        1 tgggccgcgc agcgcgcgcg cggcgggtga gcccgtggac acgatcgagc tcgccgccct
       61 gcaggcctgg ttcacgccgg tcgcgctgac gctgccgcgg ctgctcgcgg tcttcgcgat
      121 cgcgccgttc ttctccggca acatgatgag cggcctggtg cgcaacggcg tcatcctgat
      181 gctcgcgatc ttcatgtcgc ccgcggcgag cgacctgccc gtgctgacgc tgggcggctg
      241 ggtgctgatc gcggcgaagg aggccttcat cggcgtgctg ctgggcctgg gcttcggcct
      301 gttcgtctgg gcggtgcaga gcgtgggcga cctcgtggac ttccagaccg gctccgcgag
      361 cgcgcccttc ttcgacccgg tcggtggcca cgagggcggt cccacggggc agttcctcgg
      421 ctgggtcgtc atcacgctgt tcatgtccgc gggcgggatg ctcgcgatgg tgcgcgtgat
      481 cgtcgactcc ttccggctct ggccggtggc ctcgttctac ccgcacatgg gcgaggtgat
      541 cgagcagttc gccgtgcgcc agggcgacac gctgttcctg tggaccgtca agctcgccgc
      601 gcccatcatc ttcgtgctgc tgctggtgga gctgggcatc ggcctcgtcg ggcgcgtggc
      661 gccgcagctc aacgtgttcg tgttctcgca gccggtcaag agcgtgctcg ccaacctgat
      721 gctgctgctg ttcctgtact tcctctacga ctcgctgcag gagttcctga agccggagaa
      781 cggggtgctg gacttcctca ggaacacctt gtgagcgcca acaaggacca gaagccgacc
      841 gcgcgccggc tgcgcgaggc gcgcaaacgc ggcgacgtcg ccttcagctc ggacatcgcc
      901 tccgccgccg tcttcacggt cgtcgtcggc gggctgtggc tgctcggcgc gaccagcttc
      961 ggcctgctgc agcagctgtg gctgcatgcg acgagcgccg agctgctcac ccgtcccgac
     1021 gaccgcttcc ccgagctgct gcgccacacc ggccacgtgc tgctgtggat cgcgctgccc
     1081 gtcacggggc tcgcggcgct cgcggggatc gcgtcctcgt tcttccaggt cggcggcatc
     1141 ctggccttcg agcggctgaa gcccgacgtc gcgcggctcg cgccctcgcg cggcctgcag
     1201 cgcatgttct cgacgcgcaa cctgatcaac ctcgtgaaga tgctggtcaa gacgctcctc
     1261 ctgatcgtgc tcgtgggcgc gacggtgcgc gggctgctgg atgcggcgct caagctgggc
     1321 tacctgcgtc ccgacgcgat ccttgcggcg ggcgcccatg cgctgctcgg gctgttcggc
     1381 gccgccgcgg tgatctacgc ggtgctggcc ggggtcgact acgcgcacca gcactacgag
     1441 tacatcaaga gcctgcgcat gtcgatcgac gaggtgcggc gcgactacaa ggaggccgag
     1501 ggcgatccgc tcacgcgcgc gcggcgccgc ggtgcgcatt tcgaggcgat ctacgcgggc
     1561 ctgggtgacc gcgtggccgc gtcctcggcg gtgctgcatt ccgggcgggt ggccgtcgcg
     1621 ctgcagtacc tgggcgagcg cgacctgccg cgcgtgatcg cgcgcggcga gaacgaggtc
     1681 gcgatgcgcc tgcgcga
//
LOCUS       DASVLN010000264         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_283010, whole genome shotgun sequence.
ACCESSION   DASVLN010000264 DASVLN010000000
VERSION     DASVLN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283010"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..960
                     /gene="folE"
                     /locus_tag="VF453_06020"
     CDS             247..960
                     /gene="folE"
                     /locus_tag="VF453_06020"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX7687240.1"
                     /translation="MTLIRSEADLAQLPASERIRYRLVGADCRFHANDNISAYVRPGE
                     TEELKEEVARHMQAVLQALVIDTESDHNTQETARRVAKMFVDEVFRGRYQPMPSVTEF
                     PNVSRLNELMIVGPISVRSACSHHMCPILGRVWIGVLPNEHSNLIGLSKYARIVEWIM
                     TRPQIQEEAVTMLANELQERVKPDGLAIVMEADHFCMQWRGVKDMDSAMTNSVMRGAF
                     LKDPNLRREFLSLLTKKHD"
     gene            975..1406
                     /locus_tag="VF453_06025"
     CDS             975..1406
                     /locus_tag="VF453_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEX7687241.1"
                     /translation="MLVRLMYASRATAGVDQEELHAILRRSRTTNAREGVTGALCLCT
                     TGRLFIQVLEGGREAVSRLYNRIAADPRHAEVTLLLYEEIAERRFAGWAMGRVDMARL
                     NPALVLKYSQNAQLDPWSISGRATAAMFEELIATAAIAQQA"
     gene            complement(1476..>1818)
                     /locus_tag="VF453_06030"
     CDS             complement(1476..>1818)
                     /locus_tag="VF453_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7687242.1"
                     /translation="AESLAAAGHEVRTAHSAVEAQVVAAAFVPDVAILDIGLPDLDGY
                     ALAGRLRDAPWRGRLVALTGYGQAADRERALAAGFDLHVTKPADPAALLDAVDGFLRE
                     PGAGRRSAADT"
BASE COUNT          283 a          678 c          631 g          226 t
ORIGIN      
        1 ggtcaccgat cggagtctac ccagcgcctg ttgcgcaaac gctgcgcccg gccgcggacc
       61 cggcgccgcc gtccggccgc cggcgccgcg attgcccgtt caccgcgcag gcccgggcac
      121 ggcggcgcac aatgcctgcc cggccacgac ggccgcccca ccgcgcccac catgtccaac
      181 tccgaccgat cgcccgcgac gggcccgcag ccgccgtccg atcccgccac cccgtcgacg
      241 cgggcgatga cgctgatccg cagcgaggcc gacctcgccc agctgccggc gtccgagcgg
      301 atccgctacc ggctggtggg cgccgactgc cgcttccacg ccaacgacaa catctccgcc
      361 tacgtccgcc ccggcgagac cgaggagctg aaggaagagg tcgcccggca catgcaggcg
      421 gtgctccagg cgctggtgat cgacaccgag agcgaccaca acacgcagga gacggcgcgc
      481 cgcgtggcca agatgttcgt cgacgaggtc ttccgcggcc gctaccagcc gatgccctcg
      541 gtcaccgagt tcccgaacgt ctcgcggctg aacgagctga tgatcgtcgg gccgatctcg
      601 gtgcgcagcg cgtgctcgca ccacatgtgc ccgatcctcg gccgcgtctg gatcggcgtg
      661 ctgcccaacg agcactccaa cctgatcggg ctgtcgaagt acgcccgcat cgtcgaatgg
      721 atcatgacgc gcccgcagat ccaggaggag gcggtgacga tgcttgccaa cgagctgcag
      781 gaacgcgtca agcccgacgg cctggccatc gtgatggagg ccgaccactt ctgcatgcag
      841 tggcgcggcg tgaaggacat ggactccgcg atgaccaaca gcgtcatgcg cggcgcgttc
      901 ctgaaggacc cgaacctgcg gcgcgagttc ctgtcgctcc tgaccaagaa gcacgactga
      961 aggcgaggcg caccatgctg gtccgactga tgtatgcgag ccgcgcgacc gccggcgtcg
     1021 atcaggaaga gctccacgcg atcctgcgcc gctcgcgcac gaccaacgcg cgcgagggcg
     1081 tcaccggcgc gctgtgcctg tgcaccacag ggcggctgtt catccaggtg ctcgaaggcg
     1141 ggcgcgaggc cgtgagccgg ctctacaacc gcatcgccgc cgacccccgc cacgcggagg
     1201 tgacgctgct gctgtacgag gagatcgccg aacgccgctt cgccggctgg gcgatgggcc
     1261 gggtcgacat ggcacggctg aacccggcgc tggtgctgaa gtactcgcag aacgcgcagc
     1321 tcgatccgtg gtcgatctcc ggccgcgcga cggcagcgat gttcgaggag ctgatcgcca
     1381 ccgcggcgat cgcccagcag gcgtagcagc ctgcgcgcag gcccggctcg cactcgacac
     1441 cgccctgccc cgccggcttc ggaccggctc caggcctagg tgtccgctgc cgaccggcgc
     1501 ccggcgccgg gctcgcgcag gaagccgtcg accgcgtcca gcagcgccgc gggatcggcc
     1561 ggcttggtga cgtgcaggtc gaagcccgcg gcaagcgcgc gctcgcgatc ggcggcctgg
     1621 ccgtagccgg tgagtgcgac gaggcggccg cgccagggcg cgtcgcgcag ccgcccggcg
     1681 agcgcgtagc cgtcgaggtc gggcaggccg atgtccagga tcgcgacgtc cggcacgaag
     1741 gccgcggcca cgacctgagc ctcgaccgcc gagtgcgcgg tgcgcacctc gtggccggca
     1801 gcggccagcg actcggcc
//
LOCUS       DASVLN010000265         3891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_175199, whole genome shotgun sequence.
ACCESSION   DASVLN010000265 DASVLN010000000
VERSION     DASVLN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175199"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VF453_06035"
     CDS             complement(<1..577)
                     /locus_tag="VF453_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7687243.1"
                     /translation="MPCPIPASHATGSSVFDVLSSSHARLHRRAAVGDGLVIAQWSNS
                     FDTTKYQPEHHTLSLYLSGGQGTYRADAPGERGAPGKLCLMPAGHEVDWIVGPEQHFV
                     HLYFAADQLGPLALRVLDREARELALPELNFVDDRALAATLGGLVGLDWEDAGDRLAA
                     NQIGHDALVHVLARHAGRALGRRLRGGLAPAT"
     gene            755..1657
                     /locus_tag="VF453_06040"
     CDS             755..1657
                     /locus_tag="VF453_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7687244.1"
                     /translation="MTAVLYALTVAIWGTTWIALKMQLGIVPIPWSIAWRFALAALVL
                     FAWLAWRGRLRLPRREARPLVLAQGLCLFCLNFICFLHAEAEVPSGLVAVVFSTSTLW
                     NALGARLVFKRVLTPQVLGGGALGLAGLVAMFWPEIARHGASAATLRGLAFALGGTLC
                     FSCGNLLSARLQALGEPPAFTNAWGMATGTLVLALGSLAAGVAPAFDPSPAYVGAWLY
                     LAIPGSVVGFTAYLMLVGRLGPERAAYCTVLFPLVALAISAVVEGYRWTPPALAGLAL
                     VMAGNVLVFRKARPAAAASRPAAA"
     gene            complement(1667..3484)
                     /locus_tag="VF453_06045"
     CDS             complement(1667..3484)
                     /locus_tag="VF453_06045"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX7687245.1"
                     /translation="MDTRTSPARARIARLREVMQQRGLDAVLVPSSDPHLSEYLPGRW
                     QGREHFSGFTGSMGTLAVTPTKAALFADSRYWAQAETQLQGSGIALERIPTGTATNHV
                     DWLAAGVPRGGTVAVDGDVLGLAASQQLRRRLEAAGIALQTGADVLGEAWDERPALPQ
                     ARVYEHVAPYAPQPRSAKLAQVRAAMSAAGATHHFVSTVDDIAWITNLRGADVEYNPV
                     FLAHLLIAADAATLFVGTGKVAPELAAALAADGIVLADYADAGAALAALPADAVLLVD
                     PRRITLGFREQVGDGVRVVEAINPSTLAKSRKTAEELAFVREAMAQDGAAMCAFYAWF
                     DKATAADRPFGPITELTIDDKLSAERRKRPGFVGLSFPVIAGWNANGAMPHYRASPES
                     HATIEGDGLLLIDSGAQYVGGTTDITRVWPVGTISREHRLDYTRVLQGTLALSQARFP
                     RGTLSPMLDALARAPLWAAKLDYGHGTGHGVGYFLNVHEGPQSISKALPEPTMAMEAG
                     MITSIEPGLYRPGQWGIRIENLVANVSCGANAFGDFLEFETLTLCPIDTRCIEPGMLE
                     RRDVDWLNAYHAVVRERLLPLVEGEAKDWLLARTEPFAG"
BASE COUNT          458 a         1377 c         1497 g          559 t
ORIGIN      
        1 gcgtcgcggg cgcgaggccg ccgcgcaggc gccggccgag ggcgcggccg gcgtgccggg
       61 cgaggacatg cacgagcgcg tcgtggccga tctggttggc ggcgaggcgg tcgcccgcgt
      121 cctcccagtc gaggccgacg agtccgccca gcgtcgcggc gagtgcccgg tcgtcgacga
      181 agttgagctc gggcagcgcg agctcgcgcg cctcgcggtc gagaacgcgc agtgcgagcg
      241 gcccgagctg gtcggccgcg aagtacaggt gcacgaagtg ctgctccggc ccgacgatcc
      301 agtcgacctc gtggccggcc ggcatcaggc agagcttgcc gggcgcgccg cgctcgccgg
      361 gcgcgtccgc ccggtaggtg ccctggccgc cgctcaggta gagcgacagc gtgtggtgct
      421 cgggctggta cttcgtcgtg tcgaagctgt tgctccactg cgcgatcacc aggccgtcgc
      481 cgaccgccgc gcggcggtgc agccgggcgt gcgacgagga cagcacgtcg aagacgctgc
      541 tgccggtggc gtgggaagcg gggatcgggc agggcatggg gggtcgcgca ggcgcgcgcg
      601 tggcgtcgac tctaacggct cgcgggcccg cgcgggcggc cgcggcgcat gcaagcgcag
      661 gatcgtgcaa ggaccgcacg ctcgccgcga cggcgcgcgc gcatgcgcag gatcgtgcaa
      721 gccgccgcgg ccgcgcgcgg ccagactcgc gcccatgacc gccgtcctct acgccctcac
      781 cgtcgcgatc tggggcacca cctggatcgc cctgaagatg cagctcggga tcgtgccgat
      841 cccgtggtcg atcgcctggc gcttcgcgct cgcggcgctg gtgctgttcg cctggctcgc
      901 ctggcgcggc cgcctgcgcc tgccgcggcg cgaggcgcgg ccgctggtgc tcgcccaggg
      961 gctgtgcctg ttctgcctga acttcatctg cttcctgcac gcggaagccg aggtcccgag
     1021 cggcctggtc gccgtcgtct tctcgacgtc gacgctatgg aatgccctcg gcgcgcgact
     1081 cgtcttcaaa cgcgtgctga cgccccaggt gctgggcggc ggggcgctgg ggctcgcggg
     1141 gctggtggcg atgttctggc cggagatcgc gcgccacggg gcgtcggcgg cgacgctgcg
     1201 cgggctggcg ttcgcgcttg gcgggacgct gtgcttctcg tgcggcaacc tgctgtcggc
     1261 gcggctgcag gcgctcggcg agccgcccgc cttcaccaac gcctggggca tggccaccgg
     1321 caccctcgtg ctggcgttgg gcagcctggc cgccggcgtc gcgccggcct tcgacccgtc
     1381 gccggcgtac gtcggcgcgt ggctgtacct cgcgatcccc ggctcggtgg tgggcttcac
     1441 ggcctacctg atgctggtcg ggcgcctcgg gcccgagcgc gccgcctact gcacggtgct
     1501 gttcccgctc gtggcgctgg cgatctctgc cgtcgtcgag ggctaccgct ggacgccgcc
     1561 ggcgctggcc ggcctcgcgc tggtgatggc aggcaacgtg ctggtcttcc gcaaggccag
     1621 gccggcggcc gcggcatcga ggcccgcggc cgcctgagcg cgcgcgtcag ccggcgaacg
     1681 gctcggtgcg cgccagcagc cagtccttcg cctcgccctc gaccagcggc agcagccgct
     1741 cgcgcaccac tgcgtggtag gcgttgagcc agtcgacgtc gcggcgctcg agcatcccgg
     1801 gctcgatgca gcgggtgtcg atcgggcaca gcgtcagcgt ctcgaactcg aggaagtcgc
     1861 cgaacgcgtt ggcgccgcag gagacgttgg ccaccaggtt ctcgatgcgg atgccccact
     1921 ggccaggccg gtacaggccg ggctcgatcg aggtgatcat gccggcctcc atcgccatcg
     1981 tgggctcggg cagcgccttg ctgatgctct gcgggccctc gtgcacgttc aggaagtagc
     2041 cgacgccatg gccggtgcca tggccgtagt ccagcttcgc ggcccacagc ggcgcgcggg
     2101 cgagcgcgtc cagcattggc gacagcgtgc cgcgcgggaa gcgcgcctgg ctcagcgcga
     2161 gcgtgccctg cagcacgcgc gtgtagtcga ggcggtgttc ccgcgagatc gtgccgacgg
     2221 gccagacgcg cgtgatgtcg gtcgtgccgc cgacgtactg cgcgcccgag tcgatcagca
     2281 gcaggccgtc gccctcgatc gtcgcgtgcg actcggggct cgcgcggtag tgcggcatcg
     2341 cgccgttggc gttccagccg gcgatcaccg ggaagctcag gcccacgaag ccgggacgct
     2401 tgcggcgctc ggccgagagc ttgtcgtcga tggtgagctc ggtgatcggt ccgaacgggc
     2461 gatccgcggc cgtggccttg tcgaaccagg catagaacgc gcacatcgcg gcgccgtcct
     2521 gggccatggc ctcccgcacg aaggcgagct cctcggcggt cttgcggctc ttggcgagcg
     2581 tgctcgggtt gatcgcctcg accacgcgca cgccgtcgcc gacctgctcg cgaaagccca
     2641 gcgtgatgcg gcgcgggtcg accagcagca cggcgtccgc gggcagtgcg gcgagtgccg
     2701 cgccggcgtc cgcgtagtcg gccaggacga tgccgtcggc agcgagcgcg gcggcgagct
     2761 cgggcgccac cttgcccgtg ccgacgaaca gcgtcgccgc gtcggcggcg atcagcaggt
     2821 gggcgaggaa gaccgggttg tactcgacgt ccgcgccgcg caggttcgtg atccaggcga
     2881 tgtcgtcgac cgtcgagacg aagtggtgcg tcgcgcccgc ggccgacatc gcggcgcgga
     2941 cctgcgcgag cttggccgag cgcggctgcg gcgcgtaggg cgcgacatgc tcgtagacgc
     3001 gcgcctgcgg cagcgctggg cgctcgtccc aggcctcgcc gagcacgtcg gcgcccgtct
     3061 gcaacgcgat gcccgcggcc tcgaggcgac ggcgaagctg ctgcgaggcg gccaggccga
     3121 gcacgtcgcc gtcgaccgcg acggtgcccc cgcgcggcac ccccgctgcg agccagtcga
     3181 catggttcgt cgcggtgccg gtggggatgc gctccagcgc gatgccgctg ccctgcagtt
     3241 gcgtctccgc ctgcgcccag tagcggctgt cggcgaacag cgccgccttg gtcggggtga
     3301 ccgcgagcgt gcccatcgag ccggtgaagc cggagaagtg ctcgcggccc tgccagcggc
     3361 ccggcaggta ctccgacaga tgcggatcgc tcgacggcac cagcacggcg tcgaggccgc
     3421 gctgctgcat cacctcgcgc agtcgggcga tgcgggcgcg ggcgggggag gtgcgggtgt
     3481 ccatcgggat tccttcgcgg ggaggtcggt ggcgtgccgg cggtgggcac ggcgcggccg
     3541 ggtaacgggc ggcctgcgga tcgcgcgggg ccgccggagc tgttcgaggc gagcgtcgat
     3601 tctaggcgcg cgggagtgcg gcgggcgcga cgcaagaccc gcccggccga gaggctgcgc
     3661 tggcttgttg tgtgttacct tggtacataa gtacgaatgt gacacaatgc ctctcccgca
     3721 cgacggagac cggaccacca tgaccgacga cgagttcctc catggcttcg acgcgggcgc
     3781 gctgccgccc gcgcagttcg accacgccgc ccacctgcgc gcggcgcggg cgctgctgct
     3841 gcgcgcgccc ttcctcgagg cctgcgcccg gatgcgcgac ggcctgcgcg c
//
LOCUS       DASVLN010000266         5623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_67055, whole genome shotgun sequence.
ACCESSION   DASVLN010000266 DASVLN010000000
VERSION     DASVLN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67055"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1492)
                     /locus_tag="VF453_06050"
     CDS             complement(149..1492)
                     /locus_tag="VF453_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7687246.1"
                     /translation="MFTRRRPPPPSEPSAAVETARGPDFASAIRAIADQAAELGRGAA
                     ELDGVIEDVVADSTRQVSGIDRLAADMNALVASNRAIEASGDAGRVAVGQAREAVAQV
                     GEGVLGVVASLRDVARAATEITQIALQTRLVAFNATVEAKRAGEAGAGFAVVAEAVKD
                     LASKVETSSKLIMATVTQLDSRIDSLAASIAENQGTAQHSPFHAALAQVDASVNEIAA
                     ATRRNLEACQGLAAEVGETARSVAGNAESLKRVNANTRSFLKASESLAEITNDCGAVT
                     ADTPFIEAVVEGAATLSARLERAVAEGRISLADLFDERYQPVPGSDPAQVTTRFTALT
                     DELFPEVQEALLALDPKVVFCAAVDRNGYLPTHNAKFSQPQGADPVWNAANARNRRIF
                     NDRTGLAAGRNERRFLLQTYRRDMGGGQRVLMKDLSAPIVVQGRHWGGLRLAYKF"
     gene            complement(1605..2783)
                     /locus_tag="VF453_06055"
     CDS             complement(1605..2783)
                     /locus_tag="VF453_06055"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HEX7687247.1"
                     /translation="MTTAVQPMRVRAIDAFRGLNMLLMVFVNQLAGVSGVPPWAKHMK
                     ADADAMSFVDVVFPAFLFITGMAIPFALAQRAARGDDGAALQRHVLARTLGLLVLGLF
                     MVNAEEGFNPAFMRWNIGAWQLAFYACAFLVWGVYGSARPGGRDGWRAVGVLGLLVVA
                     DAYRGGADGSAWMTHQWWGILGLIGWSYLITATVFLAARGRLAWLLAATAACVGYYAA
                     AHAVPTGSKAWWAEFLDEPGLVCETSLTLLGAATSLVFFAPGARRPFARAAALALALV
                     VAGALLRPAYTISKIYGSPTWCLYSAAICIVLFAALHWLVDLRGRAGWVVVVRPMAAH
                     PLVAYLIPFVIVAAMDAMHVGLPAILDEGAVGVAFSVFVAFLVLGLTAIVTRRVRLQL
                     "
     gene            complement(2830..>5623)
                     /locus_tag="VF453_06060"
     CDS             complement(2830..>5623)
                     /locus_tag="VF453_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7687248.1"
                     /translation="APASAAAPAPAASAPVPAPAPEDKPQVVEVRGIRAAYASSLYNK
                     KTSDSIVEVVTAEDVGKLPAKNVADTVQRLPGVNISSNSGGDGAYADANRVSLRGTSP
                     ELTQTLVNGHSVGSADWFIANQGFNGGAAGRSVSYDLLPSEIVGKVTVYKTAQADLIE
                     GGATGSVDIETRSPLSLAKPVTVDGSVEAVYGTTSRRFDPQVSGLVGWKNDAQTLGVI
                     VQAFSENRHDRRDGQEFLGYNTVSFQDIYGLAPVANPSNDPAIAAQNAANAAQLATLR
                     AQHPGLDGATFANNVNATLLEMHMQRKGALVDVELKPASALRLDFNGFYSKLTATNQD
                     SSLETEQTALIGAGVLPDSYNVNGSGLLTQATWTQANMSDYASTGAYVADYDKYYRPN
                     ESARVWYADMDFDWSASDRLRVNGKLGRTQGVGDTPNEYAYVTNVGNAGLSYQMNGLY
                     VPADVSFPGYANTGNYDDPHLFLAYDGLNKTRATDSETYFQADGEYAVEAGVLDAVKF
                     GARLTSHRRDVLDYYNDGCEAVIPDDTYTQCNGIAAWSGQVENHYGQGLRGGPGFLRN
                     IWQLSPQQIVDYVNAPGNLPASSLPYSWPSSFVVQEKTQAVYGMGKFVGDHWKANAGL
                     RVVRTQQHADYNEGADAAGPGDTIHHDPHNGDYIKVRTDKTYTDLLPSVNFSYDFSRS
                     LIGRLAASRTMTRAEYTDLAGAVSLNGQNGTGSGGNPQLKPVRSNNFDATIEWYYAPQ
                     ALLEFNVFTMNLVSYIGYGQTTIYAPNLQQNPIVTTPTYPYAVAQPVNDKGTNKGFEI
                     GWQQPLPHGFGLGLNYTYAHGRDSSGAPLAGSSKNTYNAEGYFENDAFSARLTYSYRS
                     DFLTGIVSALPQYVAGSGDLSASMNLKLTSQLTLSFDAKNLTGALARQYVVDKDMPAA
                     IYNNGRQFFLGLKYSM"
BASE COUNT          775 a         1922 c         2070 g          856 t
ORIGIN      
        1 cgcgacggca cgccggccga cctgctgcgc gccgccgacg ccgcgctcta cgagggcaag
       61 cgccgcggcc gcgaccaggt gcgcgaacgc accctcgcgc cgcgccgcgc ggcttgaagc
      121 gacccggccg ccgccgcggc cgtccggttc agaacttgta cgcgagccgc aggccgcccc
      181 agtgccggcc ctgcacgacg atgggcgccg acaggtcctt catcagcacg cgctggccgc
      241 cgcccatgtc gcggcggtag gtctgcagca ggaagcggcg ctcgttgcgg ccggcggcca
      301 ggccggtgcg gtcgttgaag atgcgccggt tgcgcgcgtt cgccgcgttc cacaccggat
      361 cggcgccctg cggctgcgag aacttcgcgt tgtgggtggg caggtagccg ttgcggtcga
      421 ccgccgcgca gaacacgacc ttggggtcga gcgcgagcag cgcctcctgc acctcgggga
      481 agagctcgtc ggtgagcgcc gtgaagcgcg tggtcacctg cgcggggtcg ctgccgggca
      541 ccggctggta gcgctcgtcg aagaggtcgg ccagggagat gcgtccctcg gccaccgccc
      601 gctccagccg cgccgacagc gtcgccgccc cctcgaccac cgcctcgatg aacggcgtgt
      661 cggcggtgac ggcgccgcag tcgttggtga tctcggccag gctctccgac gccttcagga
      721 agctgcgggt gttggcgttg acgcgcttga gcgactccgc gttgcccgcg accgaacgcg
      781 ccgtctcgcc gacctcggcg gccaggccct ggcaggcctc gaggttgcga cgcgtcgccg
      841 cagcgatctc gttgacgctc gcatcgacct gcgcgagcgc ggcatggaac gggctgtgct
      901 gcgcggtgcc ctggttctcg gcgatgctgg cggccaggct gtcgatgcgc gagtccagct
      961 gcgtcaccgt ggccatgatc agcttggacg aggtctcgac cttggaagcg agatccttca
     1021 ccgcctcggc gacgaccgcg aagcccgcgc ccgcctcgcc cgcgcgcttg gcctcgaccg
     1081 tcgcgttgaa ggcgaccagc cgcgtctgca gggcgatctg cgtgatctcg gtggccgcgc
     1141 gcgcgacgtc gcgcagcgag gccacgactc ccagcacgcc ctcgccgacc tgcgcgaccg
     1201 cctcgcgcgc ctggccgacc gcgacgcggc cggcatcgcc gctcgcctcg atcgcgcggt
     1261 tggaggcgac gagtgcgttc atgtcggcgg ccaggcggtc gatccccgag acctggcggg
     1321 tgctgtcggc gaccacgtcc tcgatcacgc cgtccagctc ggccgccccg cgcccgagct
     1381 cggccgcctg gtcggcgatg gctcggatcg cgctcgcgaa gtccggcccg cgggccgttt
     1441 cgaccgcggc ggacggctcg gaaggcggcg gcgggcgccg gcgagtgaac atggggcgac
     1501 tccctggcgc gcacggcgcc gttgcggtga tcgcccaagt atgcaaacaa gcccggcccg
     1561 cggcaatgcg gggttcctga tcccgcgcac gcgatcgccc gcgttcagag ctgcagccgc
     1621 acgcggcgcg tcacgattgc cgtcagcccc aggacgagga aggcgacgaa gacgctgaag
     1681 gccacgccga cggcaccctc gtcgaggatg gcaggcagcc cgacgtgcat cgcatccatc
     1741 gccgcgacga tcacgaacgg gatgaggtag gcgaccaggg gatgcgccgc catcggccgc
     1801 acgacgacca cccagccggc gcgcccgcgc aggtcgacga gccagtgcag cgccgcgaac
     1861 agcacgatgc agatcgcggc cgagtacagg caccaggtcg gcgagccgta gatcttggag
     1921 atcgtgtagg ccgggcgcag cagggcaccc gcgacgacca gcgcgagggc gagcgccgcc
     1981 gcgcgcgcga acggccgccg cgcgccgggg gcgaagaaca cgaggctggt cgcggcgccc
     2041 agcaacgtga gcgaggtctc gcagacgagg ccgggctcgt cgaggaactc ggcccaccag
     2101 gccttggacc ccgtcggcac cgcgtgcgcc gccgcgtagt agccgacgca cgcggccgtc
     2161 gccgcgagca gccacgcgag ccgtccgcgc gcggccagga acaccgtggc cgtgatcagg
     2221 taggaccagc cgatcagccc gaggatgccc caccattggt gcgtcatcca ggcgctgccg
     2281 tcggcgccgc cgcgataggc gtcggccacc accagcaggc ccagcacgcc caccgcacgc
     2341 cagccgtcgc gcccaccggg ccgcgcgctg ccgtacacgc cccagaccag gaacgcgcag
     2401 gcgtagaacg cgagctgcca ggcgccgatg ttccagcgca tgaaggcggg gttgaagccc
     2461 tcctcggcat tgaccatgaa caggcccagc accagcagcc ccagcgtgcg cgcgagcacg
     2521 tggcgctgca gcgcggcgcc gtcgtcgccg cgcgccgcgc gctgggcgag cgcgaacggg
     2581 atcgccatgc ccgtgatgaa caggaacgcc gggaagacga cgtcgacgaa gctcatcgcg
     2641 tcggcgtcgg ccttcatgtg tttggcccag ggcggcacgc cgctgacgcc ggcgagctgg
     2701 ttgacgaaca ccatcaggag catgttgagg ccccggaagg cgtcgatggc gcgcacgcgc
     2761 atgggctgga cggcagtggt caagaaggat tcccgcgttc gaggttgcga aggggcgcgg
     2821 cggcggtgct cacatcgagt acttcagccc gaggaagaac tggcgcccgt tgttgtagat
     2881 cgccgccggc atgtccttgt ccacgacgta ctggcgcgcc agcgcgccgg tgaggttctt
     2941 ggcgtcgaag gacagcgtca gctgcgacgt gagcttgagg ttcatcgacg ccgacaggtc
     3001 gccggagccg gcaacgtact gcggcagcgc gctcacgatg ccggtgagga agtccgagcg
     3061 gtagctgtag gtcaggcgcg cgctgaaggc gtcgttctcg aagtagccct cggcgttgta
     3121 cgtgttcttc gacgagcccg ccagcggcgc gcccgacgag tcgcggccgt gcgcgtacgt
     3181 gtagttcagg ccgagcccga agccgtgggg cagcggttgc tgccagccga tctcgaagcc
     3241 cttgttggtg cccttgtcgt tgaccggctg cgcgaccgcg tacgggtagg tcggcgtcgt
     3301 gacgatcggg ttctgctgca ggttgggcgc gtagatcgtc gtctggccgt agccgatgta
     3361 cgagaccagg ttcatggtga acacgttgaa ctcgagcagg gcctgcggcg cgtagtacca
     3421 ctcgatcgtg gcgtcgaagt tgttcgagcg cacgggcttc agctgcggat tgccgcccga
     3481 gccggtgccg ttctggccgt tcaggctcac ggcgccggcg aggtcggtgt actccgcgcg
     3541 cgtcatcgtg cgcgaggcgg cgagccgccc gatcaggctg cgcgagaagt cgtacgagaa
     3601 gttcacgctc ggcagcaggt cggtgtaggt cttgtccgtg cgcaccttga tgtagtcgcc
     3661 gttgtgcggg tcgtggtgga tcgtgtcgcc cgggccggcg gcgtcggcgc cctcgttgta
     3721 gtcggcatgc tgctgcgtgc gcacgacgcg caggccggcg ttggccttcc agtggtcgcc
     3781 gacgaacttg cccatgccgt agaccgcctg cgtcttctcc tgcacgacga agctgctggg
     3841 ccacgaatag ggcagcgagc tcgccggcag gttgcccggc gcattgacgt agtcgacgat
     3901 ctgctgcggg ctcagctgcc agatgttgcg caggaagccc gggccgccgc gcaggccctg
     3961 gccgtagtgg ttctcgacct ggccgctcca cgcggcgatg ccgttgcact gcgtgtaggt
     4021 gtcgtcgggg atgacggcct cgcagccgtc gttgtagtag tcgagcacgt cgcggcggtg
     4081 gctggtgagg cgggcgccga acttgaccgc gtccagcacg cccgcctcca ccgcatattc
     4141 gccgtcggcc tggaagtagg tctcgctgtc ggtggcgcgc gtcttgttca ggccgtcgta
     4201 ggcgaggaac aggtgcgggt cgtcgtagtt gccggtgttc gcgtagccgg ggaaggagac
     4261 gtccgcggga acgtacaggc cattcatctg gtaagagagg cccgcgttgc cgacgttggt
     4321 gacgtacgcg tactcgttcg gggtatcgcc gacgccctgc gtgcgcccga gcttgccgtt
     4381 cacgcgcagg cggtcggagg ccgaccagtc gaagtccatg tcggcgtacc agacgcgcgc
     4441 gctctcgttg ggccggtagt acttgtcgta gtcggcgacg taggcgcccg tggacgcgta
     4501 gtcggacatg ttggcctgcg tccaggtcgc ctgcgtcagc agcccgctgc cgttgacgtt
     4561 gtacgagtcg ggcagcacgc ccgcgccgat cagcgcggtc tgctcggtct ccaggctcga
     4621 gtcctggttg gtcgcggtga gcttggagta gaagccgttg aagtccagcc gcagggcgct
     4681 cgcgggcttg agctcgacgt ccaccagcgc gcccttgcgc tgcatgtgca tctccagcag
     4741 cgtcgcgttg acgttgttgg cgaaggtcgc gccgtccagg cccgggtgct gcgcgcgcag
     4801 cgtggccagc tgcgcggcgt tggcggcgtt ctgcgcggcg atcgccgggt cgttgctcgg
     4861 gttggcgacc ggcgcgaggc cgtagatgtc ctggaacgag accgtgttgt agccgaggaa
     4921 ctcctggccg tcgcggcggt cgtggcggtt ctcggagaac gcctgcacga tcacgcccag
     4981 cgtctgcgcg tcgttcttcc agccgaccag gccgctgacc tgcggatcga agcggcgcga
     5041 ggtggtgccg tagacggcct cgaccgagcc gtcgaccgtc accggcttgg cgaggctcag
     5101 cggcgagcgc gtctcgatgt ccaccgagcc ggtcgcgccg ccctcgatca ggtcggcctg
     5161 cgcggtcttg tagacggtga ccttgccgac gatctccgac ggcagcaggt cgtagctgac
     5221 gctgcgcccg gccgcgccgc cgttgaagcc ctggttggcg atgaaccagt cggcgctgcc
     5281 gacgctgtgg ccgttgacca gggtctgcgt gagctcgggc gaggtgccgc gcaggctgac
     5341 gcggttggcg tcggcgtagg cgccgtcgcc gcccgagttc gagctgatgt tgacgcccgg
     5401 caggcgctgc acggtgtcgg cgacgttctt ggcgggcagc ttgccgacgt cctccgccgt
     5461 cacgacctcg acgatcgagt ccgaggtctt cttgttgtac agcgaggacg cgtaggccgc
     5521 gcggatgccg cgcacctcca cgacctgcgg cttgtcctcc ggcgcgggcg cgggcaccgg
     5581 cgcggacgcc gcgggcgcgg gtgccgccgc cgacgcgggg gcc
//
LOCUS       DASVLN010000267         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_228023, whole genome shotgun sequence.
ACCESSION   DASVLN010000267 DASVLN010000000
VERSION     DASVLN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228023"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..91)
                     /locus_tag="VF453_06065"
     CDS             complement(<1..91)
                     /locus_tag="VF453_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687249.1"
                     /translation="MRISVIGAGAWGTALAIAAARRHRVLLWAR"
     gene            complement(126..581)
                     /gene="secB"
                     /locus_tag="VF453_06070"
     CDS             complement(126..581)
                     /gene="secB"
                     /locus_tag="VF453_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX7687250.1"
                     /translation="MADQDQTPVFQIQRIYLKDLSLEQPNSPQVLLEQTQPQVDIQLG
                     LGADQVAPGVFEVTVTGTVTTKVGDRTLFLVEAKQAGIFEVRNVPGDQLQAILHVLCP
                     SMLYPYLRAIVSDICTRAGFPPILLAEVNFQAMFEAQQQAQANGAAAAN"
     gene            complement(697..954)
                     /gene="grxC"
                     /locus_tag="VF453_06075"
     CDS             complement(697..954)
                     /gene="grxC"
                     /locus_tag="VF453_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX7687251.1"
                     /translation="MNPVRMYTTQVCPYCIRAKQLLKQRGVEAIDEIRVDLHPEQRQT
                     MIAQTGRRTVPQIFIGETWVGGCDDLIALDQRGGLLPLLGA"
     gene            complement(1107..1529)
                     /locus_tag="VF453_06080"
     CDS             complement(1107..1529)
                     /locus_tag="VF453_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7687252.1"
                     /translation="MRFFLDPITWILILAVVVSGGLLFWPLVSRSRAGGISTSEAVRL
                     INREKAVVIDVREPGEFAAGRVDGSRNVPLGRLEVAPELPKNKSLPVILVCATGKRAA
                     RAAKALEAKGYGKALVLAGGVEAWRAANLPMGSDKPAA"
     gene            1633..2379
                     /gene="gpmA"
                     /locus_tag="VF453_06085"
     CDS             1633..2379
                     /gene="gpmA"
                     /locus_tag="VF453_06085"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX7687253.1"
                     /translation="MHKLVLIRHGESTWNLENRFTGWTDVELTATGVTQARTAGELLK
                     EAGYEFDVAYTSVLKRAIWTLWHCLDTMDRTWLPVVKDWRLNERHYGGLQGLNKADMA
                     RQYGDEQVLIWRRSYDTPPPALEADDPRGQRHDLRYARLAPDQIPLTECLKDTVARVL
                     PCWNEVLAPAIRSGQRVVVAAHGNSIRALVKYLDGIGDADIVGVNIPNGIPLVYELDD
                     DLKPIRSYYLGDAEAAAKAAAAVAAQGKKA"
     gene            complement(2498..>3366)
                     /locus_tag="VF453_06090"
     CDS             complement(2498..>3366)
                     /locus_tag="VF453_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7687254.1"
                     /translation="AVAEELHFTRAAQRLHMAQPPLSQQIRALEAELGVELFARTRRS
                     VALTDAGRALLDRAREMLAAAQALPAELQRVARGERGLLRIGFSSTLPLTALLRDLVA
                     AHRRACPEVALQLREMHTALQFEALQRDELDVGLVRYNEGTPDGIRLQVLRRDPLRLV
                     VPTAHRFARRRSVAIAECRDEAFIGFPPSMGTGSMPVLARLCAQAGFEPRIAQEAREA
                     TTQIGLVAAGLGIAVLPAPLDALRIEGARYVPLAGEDAVLVMSAAVRGDEASERVLSF
                     VRRAAALAGDAR"
BASE COUNT          507 a         1215 c         1214 g          430 t
ORIGIN      
        1 cacgcgccca cagcagcacg cggtggcgcc gcgcggcggc gatggccagc gccgtgcccc
       61 aggcgccggc gccgatgacg gagatgcgca tcgcgcgcgt cgcccggacc aaggcgtcgt
      121 gcgcttcagt tcgcggcggc cgcgccgttg gcctgcgcct gctgctgcgc ctcgaacatc
      181 gcctggaagt tgacctcggc cagcaggatc ggcgggaagc cggcgcgcgt gcagatgtcg
      241 gagacgatcg cgcgcaggta cgggtagagc atcgacgggc agagcacgtg caggatcgcc
      301 tgcagctggt cgcccgggac gttgcgcacc tcgaagatgc cggcctgctt ggcctcgacc
      361 aggaacagcg tgcggtcgcc gaccttggtc gtgaccgtgc ccgtgaccgt gacctcgaac
      421 acgcccggcg cgacctggtc ggcgcccagg ccgagctgga tgtcgacctg cggctgggtc
      481 tgctcgagca gcacctgcgg cgaattcggt tgctcgagcg acagatcctt caggtagatg
      541 cgctggatct ggaaaacggg agtttggtct tggtcagcca tgggatcttc caaaatgcga
      601 ggcccgccaa ccggatggtg cgggccgcga gcgcgcatta tgcgggatcc gcacgaccgc
      661 ccgcgacgcg cgagccgcgg gcgacagccg cgaacgtcag gcgcccagca ggggcagaag
      721 cccgccgcgc tggtcgagcg cgatcaggtc gtcgcagccg ccgacccagg tctcgccgat
      781 gaagatctgc ggcaccgtcc ggcgcccggt ctgggcgatc atcgtctggc gctgctcggg
      841 atgcaggtcg acgcggatct cgtcgatcgc ctcgacgccg cgctgcttga gcagttgctt
      901 ggcgcggatg cagtacgggc acacctgggt ggtgtacatg cggaccggat tcatggtctt
      961 gcggccggcg cgaagccggc ggaaatgcgt ggttgcccga ttttcgcgca acccggcgag
     1021 ggctgcgtcg cgtgcgggaa tgccctgccg gagcaaggcc ggggccctcg accgtgcggc
     1081 gcgaggccgc gcccggggtc gccccctcag gcggccggct tgtcggagcc catcggcagg
     1141 ttggccgcgc gccaggcctc gacgccgccc gccagcacca gcgccttgcc gtagcccttg
     1201 gcctcgagcg ccttggccgc gcgcgcggcg cgcttgccgg tcgcgcacac caggatcacc
     1261 ggcagcgact tgttcttggg cagctcgggc gcgacctcga gccggcccag cgggacgttg
     1321 cgcgagccgt cgacgcggcc ggccgcgaac tcgccgggtt cgcgcacgtc gatcaccacc
     1381 gccttctcgc ggttgatcag gcgcaccgcc tcggaggtgg agatgccgcc ggcgcgcgag
     1441 cgcgagacca ggggccagaa cagcaggccg ccggagacga cgacggccag gatcaggatc
     1501 cacgtgatgg ggtcgaggaa gaatcgcacg atgggccgtt cgctgaagaa gaaggttcgg
     1561 gaaaacacgc gattatagaa ttcgcgcttc ccaagacccg atccactccg cattccgcgc
     1621 gaggcccgcc ccatgcacaa gctcgtcctg atccgccacg gcgaatccac ctggaacctc
     1681 gagaaccgct tcaccggctg gacggacgtc gagctgaccg ccaccggcgt gacgcaggcc
     1741 cgcacggccg gagagctgct caaggaggcc ggctacgagt tcgacgtcgc ctacacctcc
     1801 gtgctcaagc gcgcgatctg gacgctctgg cactgcctgg acacgatgga ccgcacctgg
     1861 ctgccggtcg tcaaggactg gcgcctgaac gagcgccact acggcggcct gcagggcctg
     1921 aacaaggcgg acatggccag gcagtacggc gacgagcagg tgctgatctg gcgccgcagc
     1981 tacgacacgc cgccgccggc gctcgaggcg gacgacccgc gcggccagcg ccacgacctg
     2041 cgctacgccc ggctcgcgcc cgaccagatc ccgctgaccg agtgcctgaa ggacaccgtc
     2101 gcgcgcgtgc tgccgtgctg gaacgaggtg ctggcgccgg cgatccggtc gggccagcgc
     2161 gtcgtcgtcg cggcgcacgg caactcgatc cgcgcgctcg tgaaatacct ggacggcatc
     2221 ggcgacgccg acatcgtggg cgtcaacatc cccaacggca tcccgctggt ctacgaactc
     2281 gacgacgacc tgaagccgat ccgcagctac tacctcggcg acgcggaagc cgccgcgaag
     2341 gccgccgcgg ccgtggcggc ccagggcaag aaggcctgag ggactgaccg cccggctcgc
     2401 gccggctcga tccggaacgg ccgcgcgcac gtgggcgtgc cgtcggcccc gcttgcaccg
     2461 atcccagcag atccgcttcg aacgaggatg atgcgtgtca gcgggcgtcg cccgccagcg
     2521 ccgcggcgcg ccgcacgaag ctgagcacgc gctccgaggc ttcgtcccca cgcaccgcgg
     2581 ccgacatcac gagcaccgcg tcttcgcccg ccagcggcac gtagcgcgcg ccctcgatgc
     2641 gcagcgcgtc cagcggcgcc ggcagcaccg cgatgcccag gccggcggcg acgaggccga
     2701 tctgcgtcgt cgcctcgcgc gcttcctgcg cgatccgggg ctcgaagccg gcctgcgcgc
     2761 acagtcgcgc gagcacgggc atcgagcccg tgcccatgct cggcggaaag ccgatgaagg
     2821 cctcgtcgcg gcactccgcg atcgccaccg agcgccggcg cgcgaagcgg tgcgcggtcg
     2881 gcacgaccag ccgcagcggg tcgcggcgca acacctgcag gcggatgccg tcgggcgtgc
     2941 cctcgttgta gcggacgagg ccgacgtcga gctcgtcgcg ctgcagcgcc tcgaactgca
     3001 gtgccgtgtg catctcgcgc agctgcagcg cgacctccgg gcatgcgcgc cggtgcgcgg
     3061 ccacgaggtc gcgcagcagc gccgtcagcg gcagcgtcga cgagaagccg atccgcagca
     3121 gcccgcgctc gccgcgtgcg acgcgctgca gctcggctgg cagggcctgg gcggccgcga
     3181 gcatctcgcg cgcgcgatcg agcagcgccc ggccggcgtc ggtcagcgca acgctgcgcc
     3241 gcgtgcgcgc gaagagctcg acgcccagct cggcctcgag cgcgcggatc tgctggctga
     3301 gcggcggctg cgccatgtgc aggcgctggg ccgcgcgcgt gaagtgcagc tcctcggcga
     3361 cggcga
//
LOCUS       DASVLN010000268        14157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_498764, whole genome shotgun sequence.
ACCESSION   DASVLN010000268 DASVLN010000000
VERSION     DASVLN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498764"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1387)
                     /locus_tag="VF453_06095"
     CDS             complement(77..1387)
                     /locus_tag="VF453_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687255.1"
                     /translation="MRAFPPRAALALTLACGLALAGTAVAATPAPDAAASQPADAVRP
                     EFATIFNAAQDLLLKQGKAPDALAKIKEAEALPNLSPYEQYLILRVKAPAEYAANDYP
                     ASIADFEKLLPDSRLAPADRTPLLRVLINMLVDQKQWAKAAQWFPQYFSAAGEDPRMR
                     ELQAEVLYRVPDYPAAAKALQSIIDTETAAGHAPTEKQLRLLLSAQSQDGDDKGREAT
                     LERLAIAYPKADVWREITSRAAASSTKLNDREYVDVYRLKTAAFDGTPDGERLSYAAL
                     AQRAGYPGEAKRVIEEGKAHDAFKGNDAAEANKLLAAVTKGAAADRQQAAANEASARS
                     AKDGNALVGQGLLSAVDGDAAHGAQLIQQGIDKGGLKSVDEARLHLGYAQYLAGDYAA
                     AAKTFQTVAPAGNTGALAHAWSLLAQSKAQPHPAAAPAASAAAK"
     gene            complement(1584..2324)
                     /locus_tag="VF453_06100"
     CDS             complement(1584..2324)
                     /locus_tag="VF453_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7687256.1"
                     /translation="MSALAQLLNTLDPEGSQPLYQQLQRALREAIEKRVLGPDDALPS
                     ERQLAADLGVSRITVRKAIQGLADEGFLDQRQGSGNFVSTRIDKNFAKLTSFSEDMRA
                     RGRTPRSEWIKRSTGTVTPEEAMKLALSPGSTVFRFHRLRYADDAPMALEYATLSAAA
                     LPSLESVDNSLYDALERAGNRPVRALQRLRALLLNEEQARLLGAKVGDAGLLVERLGY
                     LRDGRAVELSQSIYRGDTYDFVAELNAS"
     gene            2725..5496
                     /locus_tag="VF453_06105"
     CDS             2725..5496
                     /locus_tag="VF453_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7687257.1"
                     /translation="MKTKLTPIAAAAAVVFAGAATPAFAQSAAAPASAASAAAKEDVQ
                     RVEVTGIRASLQTSINQKRNAESHVEVITAEDIGKLPDKNVADSLAHVPGVTISSAGA
                     NEGGFDESDRVSMRGTSPSLTQTLINGHNVATGDWFVLDQTGTVGRSVSYTLLPTEIV
                     SSVVVHKSSEASLVEGGVAGSVDIITRKPLEFSKPLSAAVSVGAVYAQLPAKTDPQVS
                     GLVNWKNEAGTLGVLIQGFYEKRHLRRDGIEVLGYDTIQANDASAKAGLPGLTAGVKY
                     PDLIGDALFLQERKRTGGLIDVEMKPNSVTTLDLNGFMSDMTATNINRNYMFFGRKLG
                     GGQVPTEYTVDNGYLTSATYSNSAGMMAGIYDQIARPKEGSHSQYLDFDGKFQLTDKF
                     NLKTQIGFSSGYGKTPEQDVAETVVGSTGASFKLNGPTSAANFGLGNATYNSQGSVGL
                     NDTFNTDSGDGWIFGDQNLKVKDGEQWAQLDAEYDVEVGPLVDIKFGGRGAQHTRSLW
                     GVIGQGPTVDWSLPDLAAACANGHDFRCIPAASGNYPSNFANGLTSAIPHDMWTFSPA
                     QLAAYDAKFTNRDPITRADYGADYGLRETDTAGYIQGDLEGNKWSGNFGLRIVNTKEQ
                     IQNYVAHATAQPGDVTTSAFGNFALTTTTHSYTDLLPSLNLKFDLSKDMVARFAASRT
                     MTRADYSQLAGSVSLGAVDDVNLIGTGSGGNPDLKPITSNNADLSFEWYFAPRALLSA
                     SLFYMDVTSYIGLSPVHRTYTYTDNAHPNGVPVDFTLTVPNNISAKVHGVELAWQQPV
                     WGNFGVDANYAFTTSSTADGSPMVGTSKNTYNVGGYFENDFLNARVTWNHRSAFYSGL
                     DRATAYYQGAAGDLTASVGWTFNKNFNVTLDGRNLNNPKLRYYEQGGQPRAIYENGRQ
                     FYLTAHVSY"
     gene            5679..6701
                     /locus_tag="VF453_06110"
     CDS             5679..6701
                     /locus_tag="VF453_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX7687258.1"
                     /translation="MTASPPSLPEWHGVDRARFEAEVLPRDEPAILRGLAAGWPAVAR
                     GRESPQAIARYLAALDQGHPSAAVMTRPEIEGRLGYDATMEGFNFLRQPRTVTQVLEQ
                     VLRYARLERAPAVAMQSAKIAQCLPGFTSENPMPLLDERVEPRAWIGSAIVTPAHFDE
                     SHNVAVCVAGRRRFTVFPPEQVANLYVGPLDNTPAGMPMSLVDFAKPDLERFPRFREA
                     WASARIADLEPGDAIYLPPLWWHHVQSLERFNMLVNYWWRAAAPGRAVPPFTLDALMH
                     AAATVRALPDAQRRAWRHLFEHYLFDVDRDVAGHIPPERQGVLGPMSAEEHAQLRALL
                     AQRLSR"
     gene            6903..9683
                     /locus_tag="VF453_06115"
     CDS             6903..9683
                     /locus_tag="VF453_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7687259.1"
                     /translation="MLFQRDPIAAAAALALLGALVPAQVFAQAATAAPAPASAASAAA
                     KDDIQRVEVTGLRASLEASLSRKRNAESVVEVVTADDIGKLPDKNVADSIQRVPGVNI
                     ASSAGGEGGFDENDRVSIRGTSPSLTQTLINGHAVATGDWFILDQQGVVGRSVSYSLL
                     PSEIVRTVTVAKSATADLPEGGVAGAVNIETRQPLDFKKPLTVEASVQGVYADLARKT
                     DPQLNALAGWKNDAGTFGVLVQAFSETRHERRDGLETFGYAPIDPGSPAAQAHPDLAG
                     VIAPDGVNSALFEQTRKRVGGLLDLEARPIDSLTVDLNGFYSKLDASNYNRSFYAAPA
                     NLLGFAVPDSYKVVDNTLVSANFSPAAVAAGMAAGGPDNIGAEVPAEVDSLYRPHEGA
                     QTWYLDLNGKYRASDDLTLKGDVGYTRGVGRTPNEYDYFAGIGGDGTVGMNYALHGMG
                     AADLSFPGTDVSNFNASAFTEGGAYDKVYTYDSEKYASLDAELAVDSGVLESLKFGGR
                     YTQHARSTVWPVNDSCQPPSGFQPGPCDNTAAAPRPAWSGGTYPGNFGNGFGAGAGFL
                     RHVWQLDPGAIEAFDKQYANSGGFASTENWQSEFAVKERDAAIYGMANLTGTRWSGNV
                     GLRVVRTNQSSLSNVSDNSGDTVLTPADNSHGLFTPTQIRHDFTDFLPSANFKFDLTK
                     ELVARVAAARTMARADYSALAGAVSLDDTTHTGNGGNPNLKPVRSNNLDATLEWYFAP
                     KSLLSAGVFLMDITSYVDYGTSQSVYLDQTHHQLATYTITAPVNIRAKNKGFELGYQQ
                     ALQWNLGVVANYTYTDGKTSSGAEMVGNSKNTYNLEAYYDDDTFSTRIAYTYRSAFLA
                     GLNSSFAQHEDGVGDLALSAEYKLNKNVTLTFDALNLNNPTLKYYGENRGQLEALYTS
                     GRQFYLGARFSL"
     gene            complement(10135..11352)
                     /locus_tag="VF453_06120"
     CDS             complement(10135..11352)
                     /locus_tag="VF453_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEX7687260.1"
                     /translation="MNKPPAVSLSGTNLEFARSHNRRVVMEAIRLHGRLTRADIARLT
                     ALTPQTISNIASELLKLGFLTALTPERGTRGQPATPFVINPDGAYSIGLQIDQNTMLA
                     VVVDLSGKVRGRCTLAVDHPTPAQALPLVRDVVETLRKSSDADWERVLGLGLAVPGPF
                     DVEGLSAVGPTALPGWQDLSIAQQVGNAIGLPVIMENDATAAAIGERLHGVARTFRSF
                     TYLFIGTGLGAGMFHDGHLYKGSGHNAGEVGHMIVVPDGRACPCGNRGCLERYVSLQA
                     AYEALELGDVAHATPQMLLDPSPQARARLDAWLDEAAQRLRQAVNILESLLDAEAVVI
                     GGFLPSSVLALLIERLEPLYTSVAARRNRRTPRVIAGLAGHDTAALGAAALPIFDELN
                     PNIGVLLKADAHG"
     gene            11806..13155
                     /locus_tag="VF453_06125"
     CDS             11806..13155
                     /locus_tag="VF453_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7687261.1"
                     /translation="MRQRHGFQSRLRAALGVAARGLAAVAFALACDAAAAKPVELSMW
                     VMPVTLNAQHDVPALLQPWLARHPGVTVRVTVLDWESGWNKITAAAASRRGPDLLELG
                     TTWMPAIAAMGGLEQLTDAQLAEVDHGRAYFPELWKTTQVFGKPGVYGLPWYADVRAA
                     WYRTDAFAQAGVDPKQAFATWDSFRAALRKVNGQVVAGRRVAALAYAGKNDWDVVHNL
                     APWIWNAGGDLLTPDKRHSALDQPAALKAIDFYSRLAVDGLVPPDALEKDSDILEGAF
                     VAGDYAVIFSGPWLLRRILAAPADAPVRTHFDVAPYPAGPAGRATIFSGSNLAIFKGS
                     RHKAEAWDLLKYLGDKPAQVELARLASMMPTRLDAAMDPSLQARPAFAKITAIALDGR
                     AYPPIPAWGPLEAVYTKHLGQLAELTSGISGTYSVAAMKAEIARTVKEADKVLEEAN"
     gene            13152..14105
                     /locus_tag="VF453_06130"
     CDS             13152..14105
                     /locus_tag="VF453_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7687262.1"
                     /translation="MSAASARRQERRPRRFDAFPYLLLAPAALMMLVVNLVPVVQGLR
                     MSLLKLNQYTLSQYMDAPFAGLRNYRGVLLDPDDPVHAGMLLALRNTALYALLVTACS
                     VGLGLGVAMLLNREFAGRSLARTLLMLPWVVPTYVVGTLWGFMWQRDEGIVNHVLVDV
                     LHLMDHKPFWLIGGNTFFAIVIPTVWRAWPVLMLIFLAALQAVPEELYEAARLDGANA
                     WRRFTHVTLPALKPVIVIQVMFQIVDNVYSYNIVAMMFGKGAGYAGEWGDLLMPLLTR
                     QSFSYWRFGEGAAVSFVMMALMLVFVGVWMRAFRRSMTSEG"
BASE COUNT         2206 a         5030 c         4842 g         2079 t
ORIGIN      
        1 cgacagcgcg ggcgacgcct accgcctcag cgcctgaaga cgcaaccggc cgcgcaggca
       61 gcggccggtg cccgcgtcac ttggcggccg cggaggccgc cggcgccgcg gccggatggg
      121 gttgcgcctt cgattgcgcg agcagcgacc acgcgtgcgc gagggcgccc gtgttgccgg
      181 cgggcgcgac ggtctggaag gtcttggccg cggcggcgta gtcgccggcc aggtactgcg
      241 cgtagccgag gtgcaggcgc gcctcgtcga ccgacttcag gccgcccttg tcgatgccct
      301 gctggatcag ctgcgcgccg tgggcagcgt cgccatcgac cgccgacagc aggccctgcc
      361 cgaccagcgc gttgccgtcc ttcgccgagc gtgccgaggc ctcgttggcc gccgcctgct
      421 ggcggtccgc ggcggcgccc ttggtgacgg cggcgagcag cttgttcgcc tcggccgcgt
      481 cgttgccctt gaaggcatcg tgcgccttgc cttcctcgat cacccgcttg gcctcgcccg
      541 ggtagccggc gcgctgcgcc agcgcggcgt agctcaggcg ctcgccgtcg ggcgtgccgt
      601 cgaacgcggc cgtcttcaag cggtagacgt ccacgtactc gcggtcgttc agcttggtcg
      661 acgaggccgc ggcgcggctc gtgatctcgc gccagacgtc ggccttcggg taggcgatgg
      721 ccaggcgctc cagcgtcgcc tcgcggccct tgtcgtcgcc gtcctgcgac tgcgcgctga
      781 gcagcaggcg cagctgcttt tccgtcggcg catggccggc ggcggtctcg gtgtcgatga
      841 tggactgcag cgccttggcg gcggcgggat agtcgggcac gcggtacagc acctcggcct
      901 gcagctcgcg catgcgcgga tcctccccgg cggcggagaa gtactgcggg aaccactgcg
      961 cggccttcgc ccactgcttc tggtcgacga gcatgttgat cagcacgcgc agcagcgggg
     1021 tgcggtcggc cggcgcgagg cggctgtcgg gcagcagctt ctcgaagtcg gcgatgctgg
     1081 cgggatagtc gttggccgcg tactcggccg gcgccttcac gcgcaggatc aggtactgct
     1141 cgtacggcga caggttgggc agcgcctcgg cttccttgat cttggcgagc gcgtcgggcg
     1201 ccttgccctg cttgagcagc aggtcctgcg ccgcgttgaa gatcgtcgcg aactccgggc
     1261 gcacggcgtc ggcgggctgc gatgcggcgg cgtccggcgc gggcgtcgcg gcgacggcgg
     1321 tgccggccag ggcgaggccg caggccagag tcagcgccag cgcggcgcgc ggggggaagg
     1381 ctctcattgt cggttccagg gcatgcgccg aacgtgtagg ggaccggtgc gcgcgggcgg
     1441 cgtccctcgc gcgggcgaaa actcgtattg tcgtcaaacg cgcgaagcgc ccgcctggcc
     1501 gaccgtttcg acttgaaacg acaagcccgc ggcatcgcgc gcgcagggcc cggcgcacgg
     1561 caggacgcat gcctgccgcg ggatcaggag gcgttgagct ccgccacgaa gtcgtaggtg
     1621 tcgccgcggt agatcgactg cgacagctcc accgcgcggc cgtcgcgcag gtagcccagg
     1681 cgttcgacca gcagccccgc gtcgcccacc ttcgcgccga gcagccgcgc ctgctcctcg
     1741 ttgagcagca gggcgcgcag gcgctgcagc gcgcgcacgg gccggttgcc cgcgcgctcc
     1801 agggcgtcgt agagcgagtt gtcgacggac tcgagcgagg gcagcgccgc ggccgagagc
     1861 gtcgcgtact cgagggccat cggcgcgtcg tcggcgtagc gcaggcggtg gaagcggaag
     1921 acggtcgagc ccgggctgag cgcgagcttc atcgcctcct ccggcgtcac ggtaccggtc
     1981 gaacgcttga tccattcgct gcgcggcgtg cggccgcggg cgcgcatgtc ctccgagaac
     2041 gaggtcagct tggcgaagtt cttgtcgatg cgcgtcgaga cgaagttgcc cgagccctgg
     2101 cgctggtcga ggaagccctc gtcggcgagg ccctggatcg ccttgcgcac cgtgatgcgc
     2161 gagaccccga ggtcggcggc gagctggcgc tccgacggca gcgcgtcgtc gggcccgagg
     2221 acgcgcttct cgatcgcctc gcgcagcgcg cgctgcagct gctggtacag cggctggctg
     2281 ccctcgggat cgagcgtgtt gagaagttgt gccagcgcgc tcatagcgtg cccttttctt
     2341 ggggttccca gggtcgtctc gcgggggctg tcaattgggc aaagccggat cgacaggcgg
     2401 acgcatcatg ccgcggagca taccactcag gcaccaaacc gaagaaacgc gcaccgaccc
     2461 gggaagcggg tgcgccttcc tcgtgccaag tgtgaattgt tcgcatccgc cgaggtgtac
     2521 gcattgttgc cgatgcgcca tatcagggta aacgcataga gccactgtct taactggttg
     2581 tacagtggta ctacccgatc gaataccacg aaccccagcg ttcctagccc gagggtgcac
     2641 cgtctgcgcg agagagacgg aacgacccaa tcccaaccgg cccgcgttcc cgaaaaagcg
     2701 cggccacccg aaaagaggag tcccatgaag acgaagctca cgcccatcgc agccgctgcg
     2761 gcggttgtat tcgccggcgc ggccacgccg gcgtttgcgc agtccgctgc ggcccccgct
     2821 tccgccgcgt ccgccgccgc gaaggaggat gtccagcgag tcgaggtgac gggcatccgg
     2881 gcctcgttgc agacctcgat caaccagaag cgcaacgccg agtcgcacgt cgaagtgatc
     2941 accgccgagg acatcggcaa gctgccggac aagaacgtcg ccgactcgct cgcgcacgtc
     3001 ccgggcgtga ccatcagttc ggcgggcgcc aacgaaggcg gcttcgacga gagcgaccgc
     3061 gtctcgatgc gcggcacgtc gccttcgctg acgcagacgc tgatcaacgg gcacaacgtc
     3121 gccacgggcg actggttcgt gctcgaccag accggcacgg tcggccgcag cgtctcgtac
     3181 accctgctgc cgaccgagat cgtcagcagc gtcgtggtgc acaagagctc cgaggcgagc
     3241 ctcgtcgaag gcggcgtggc cggttccgtg gacatcatca cccgcaagcc gctcgagttc
     3301 agcaagccgc tgtcggccgc ggtcagcgtg ggcgcggtct acgcgcagct gcccgccaag
     3361 accgacccgc aagtcagcgg cctcgtgaac tggaagaacg aggccggcac cctcggcgtg
     3421 ctgatccagg gcttctacga gaagcgccac ctgcgccgcg acggcatcga ggtgctgggc
     3481 tacgacacca tccaggcgaa cgacgcgtcg gcgaaggcgg gcctgcccgg gttgaccgcc
     3541 ggcgtgaagt atcccgacct catcggcgac gcgctgttcc tgcaggagcg caagcgcacc
     3601 ggcggcctga tcgacgtcga aatgaagccc aattcggtca cgacgctgga cctgaacggc
     3661 ttcatgtccg acatgacggc gacgaacatc aaccgcaact acatgttctt cggccgcaag
     3721 ctcggcggcg ggcaggtgcc gacggagtac acggtcgaca acggctacct gacttcggcg
     3781 acgtacagca actccgccgg catgatggcc ggcatctacg accagatcgc gcggccgaag
     3841 gaaggctcgc actcgcagta cctggacttc gacggcaagt tccagctgac cgacaagttc
     3901 aacctcaaga cccagatcgg cttctcgagc ggctacggca agacgccgga gcaggatgtc
     3961 gccgagaccg tggtgggcag cacgggggcg agcttcaagc tgaacggccc gactagcgcg
     4021 gcgaacttcg gcctcggcaa cgcgacctac aactcgcagg gcagcgtcgg cctgaacgac
     4081 accttcaaca ccgactcggg cgacggctgg atcttcggcg accagaacct caaggtcaaa
     4141 gacggcgagc aatgggcgca actggatgcc gagtacgacg tcgaggtcgg cccgctggtc
     4201 gacatcaagt tcggcgggcg tggcgcccag cacacgcggt cgctgtgggg cgtcatcggc
     4261 cagggcccga cggtggactg gtcgctgccc gacctggctg ccgcctgcgc gaacggccac
     4321 gacttccgct gcatcccggc cgcctcgggc aactatccga gcaacttcgc caacggcctg
     4381 acgagcgcca tcccgcacga catgtggacg ttctcgccgg cccagctcgc ggcctacgac
     4441 gcgaagttca cgaatcgtga cccgatcacc cgcgcggact acggcgcgga ctacggcctg
     4501 cgcgagacgg acaccgccgg ctacatccag ggcgacctgg aaggcaacaa gtggtcgggc
     4561 aacttcggcc tgcgcatcgt caacacgaag gagcagatcc agaactacgt cgcgcacgcc
     4621 acggcccagc ccggcgacgt caccacgtcg gccttcggca acttcgcgct gacgacgacg
     4681 acccattcgt acaccgacct tctgcccagc ctgaacctga agttcgacct gagcaaggac
     4741 atggtcgccc gcttcgcggc gtcgcggacg atgacccgcg cggactactc gcagctggcc
     4801 ggctccgtgt ccctcggcgc ggtggacgac gtcaacctga tcggcacggg cagcggcggc
     4861 aaccccgacc tgaagccgat cacgtcgaac aacgcggacc tgagcttcga gtggtacttc
     4921 gctccgcgcg cgctgctgtc ggccagcctg ttctacatgg acgtgaccag ctacattggt
     4981 ctgtcgcccg tgcatcggac ctacacctac accgacaacg cgcacccgaa cggcgtgccc
     5041 gtggacttca ccctgacggt gccgaacaac atctcggcga aggtccacgg cgtcgagctc
     5101 gcgtggcaac agccggtgtg gggcaacttc ggcgtcgatg ccaactacgc cttcacgacc
     5161 tccagcaccg cggacggctc gccgatggtc ggcacctcga agaacaccta caacgtcggc
     5221 ggctacttcg agaacgactt cctgaacgcc cgcgtgacgt ggaaccaccg ctccgccttc
     5281 tacagcggcc tggaccgcgc cacggcgtac taccagggcg ccgccggcga cctcaccgcg
     5341 tcggtgggct ggacgttcaa caagaacttc aacgtcacgc tcgatggccg caacctgaac
     5401 aacccgaagc tgcgctacta cgagcagggt ggccagccgc gtgcgatcta cgagaacggc
     5461 cggcagttct acctgacggc gcacgtctcg tactgaccgc tcccgcggcg tccgcgcgaa
     5521 ccatccgcgc ggacgcgtcg agcaggcgca gggcgacctc cgggtcgtcc tgtttttttt
     5581 tggcgatccc tcggcccgcc tccgcggcgc cccggcgcgc ggctatgctg gcgcatcgcc
     5641 cgcatgcctg ccgacccgtc ctccgaacgc gaggcccgat gaccgcctcc cctccttccc
     5701 tgcccgaatg gcatggcgtc gaccgcgcac ggttcgaagc cgaggtcctg ccgcgcgacg
     5761 agcccgcgat cctgcgcggc ctggccgcgg gctggcccgc ggtcgcgcgc ggccgcgagt
     5821 cgccgcaggc gatcgcccgg tacctggccg ctcttgacca gggccatccc agcgccgcgg
     5881 tgatgacgcg gcccgagatc gagggccggc tcggctacga cgcgacgatg gagggcttca
     5941 acttcctgcg ccagccgcgc accgtcacgc aggtgctgga gcaggtgctg cgctacgccc
     6001 gcctcgagcg cgcgccggcg gtggcgatgc agagcgcgaa gatcgcgcaa tgcctgccgg
     6061 gcttcacctc cgagaacccg atgcccctgc tggacgagcg cgtcgagccg cgggcatgga
     6121 tcggttcggc catcgtcacg ccggcgcatt tcgacgagtc gcacaacgtc gccgtctgcg
     6181 tcgcggggcg ccgccgcttc accgtgttcc cgccggagca ggtcgccaac ctgtacgtcg
     6241 gccccctgga caacacgccc gccggcatgc ccatgtcgct ggtggacttc gccaagcccg
     6301 acctcgagcg cttcccgcgc tttcgcgagg cctgggccag cgcgcggatc gccgatctcg
     6361 agccgggcga cgccatctat ctgccgccgc tgtggtggca ccacgtgcag tcgctcgagc
     6421 gcttcaacat gctggtgaac tactggtggc gggcggcggc cccggggcgc gccgtgccgc
     6481 cgttcacgct cgacgcgctg atgcatgcgg cggcgaccgt gcgcgcgctg cccgatgcgc
     6541 agcgccgggc ctggcggcac ctgttcgagc attacctgtt cgacgtcgat cgcgacgtcg
     6601 ccggccacat cccgccggag cggcaggggg tgctcggccc gatgagcgcc gaggagcatg
     6661 cgcagttgcg cgcgctgctg gcccagcggc tctcgcgctg aaggcgccgc atccgattcg
     6721 gccgccgcgc ggcccggcgc tcgcggccga atcattcgcc ggcggcgcgt ccccggcatc
     6781 gtcgaaccga aggcctccct cggtggcgtc cgcgacggat cagtgaaaac cagcgatacg
     6841 aggaggaggt ggaaccgtat gatttcatta aatcaactca tttgatttga tggaatcaag
     6901 caatgctctt ccaacgagac ccgatcgccg ctgccgcggc gctggccctg ctcggcgcgc
     6961 tcgtgccggc gcaggtgttc gcacaagccg ccaccgccgc cccggcgcct gcgtccgccg
     7021 cgtcggccgc cgccaaggac gacatccagc gcgtcgaggt caccggcctg cgcgcctcgc
     7081 tcgaggcctc gctgtcgcgc aagcgcaatg cggagtcggt cgtcgaggtg gtcaccgcgg
     7141 acgacatcgg caagctcccc gacaagaacg tcgccgactc gatccagcgc gtgcccggtg
     7201 tcaacatcgc gtcgtcggcg ggcggcgaag gcggcttcga cgagaacgac cgcgtcagca
     7261 tccgcggcac cagccccagc ctcacgcaga cgctgatcaa cggccacgcg gtggcgacgg
     7321 gcgactggtt catcctcgac cagcagggcg tggtgggacg cagcgtgagc tactcgctgc
     7381 tgccgtcgga gatcgtgcgc acggtcaccg tcgccaagag cgcgaccgcg gacctgcccg
     7441 agggcggcgt cgccggcgcg gtgaatatcg agacgcgcca gccgctggac ttcaagaagc
     7501 cgctgacggt cgaggcctcg gtgcagggcg tctacgccga cctcgcccgc aagaccgacc
     7561 ctcagctcaa cgcgctggcc ggctggaaga acgacgcggg caccttcggc gtactggtgc
     7621 aggccttctc cgagacccgc cacgagcgcc gcgacggcct cgagaccttc ggctacgcgc
     7681 ccatcgaccc gggctcgccg gccgcgcagg cgcatccgga ccttgccggc gtcatcgcgc
     7741 ccgacggcgt caactccgcg ctgttcgagc agacccgcaa gcgcgtcggc gggctgctgg
     7801 atctcgaggc caggccgatc gacagcctga cggtggacct gaacggcttc tactcgaagc
     7861 tggacgcgag caactacaac cgcagcttct acgccgcccc ggccaacctg ctggggttcg
     7921 cggtgccgga ctcgtacaag gtcgtggaca acacgctggt gtcggcgaac ttctcgcccg
     7981 cagccgtcgc ggcgggcatg gccgccggcg gccccgacaa catcggcgcc gaggtgcccg
     8041 ccgaagtgga ctcgctctac cgcccccacg agggcgcgca gacctggtac ctcgacctga
     8101 acggcaagta ccgcgcgagc gacgacctca cgctcaaggg cgacgtcggc tacacccgcg
     8161 gcgtcggccg cacgcccaac gagtacgact acttcgccgg catcggcggc gacggcacgg
     8221 tcggcatgaa ctacgcgctg cacgggatgg gcgcggccga cctgtccttc cccggcaccg
     8281 acgtctccaa cttcaacgcg agcgccttca ccgagggcgg cgcctacgac aaggtctaca
     8341 cctacgacag cgagaagtac gcctcgctgg acgccgagct cgcggtggac tcgggcgtgc
     8401 tcgagtcgct gaagttcggc ggccgctaca cgcagcatgc gcgcagcacg gtctggccgg
     8461 tcaacgacag ctgccagccg cccagcggct tccagcccgg cccgtgcgac aacaccgccg
     8521 ccgcaccgcg gccggcctgg tcgggcggca cctacccggg caacttcggc aacggcttcg
     8581 gcgccggcgc gggcttcctg cgccacgtct ggcagctcga cccgggcgcg atcgaggcct
     8641 tcgacaagca gtacgccaac agcggcggct tcgcgagcac cgagaactgg cagtccgaat
     8701 tcgccgtcaa ggagcgcgac gccgcgatct acggcatggc caacctcacc ggcacgcgct
     8761 ggagcggcaa cgtgggcctg cgcgtcgtgc gcacgaacca gagctcgctg tccaacgtct
     8821 ccgacaacag cggcgacacg gtgctgacgc cggccgacaa ctcccacggc ctgttcacgc
     8881 cgacgcagat caggcacgac ttcaccgact tcctgcccag cgcgaacttc aagttcgacc
     8941 tgacgaagga gctcgtcgcc cgcgtcgccg cggcgcgcac gatggcgcgc gcggactaca
     9001 gcgcgctggc cggcgcggtc tcgctcgacg acacgaccca caccggcaac ggcggcaacc
     9061 ccaacctgaa gccggtgcgc tcgaacaacc tcgacgcgac gctcgagtgg tacttcgcgc
     9121 cgaagtcgct gctgtccgcg ggcgtgttcc tgatggacat cacgtcctac gtcgactacg
     9181 gcacctcgca gtcggtctac ctcgaccaga cccaccacca gctcgcgacc tacacgatca
     9241 ccgcgccggt caacatccgc gcgaagaaca aggggttcga gctcggctac cagcaggccc
     9301 tgcagtggaa cctgggcgtc gtcgccaact acacgtacac cgacggcaag acctcctcgg
     9361 gcgcggagat ggtgggcaac tcgaagaaca cgtacaacct cgaggcgtac tacgacgacg
     9421 acacgttctc gacgcgcatc gcgtacacgt accgctcggc cttcctcgca ggcctgaact
     9481 ccagcttcgc ccagcacgag gacggcgtcg gcgacctcgc gctgtcggcc gagtacaagc
     9541 tcaacaagaa cgtgacgctg accttcgacg cgctcaacct gaacaacccg acgctgaagt
     9601 actacggcga gaaccgcggc cagctcgagg cgctgtacac gagcggtcga cagttctacc
     9661 tgggggcgcg cttctcgctc tgacgagggt gccgtggccg tcgcccggcg ggcgcgagcg
     9721 cgccggcccg cgccggaggg acgcgcggca tgcgcgctcc gcgccccgcc cattttcatg
     9781 cagaccagcc aacgttccgg cgcgcgcgcg ccgccgcggg tggatcagcg tttttaccga
     9841 gtaccagtct agccactggt agtacattgg tgccatgttg tgacgtgagg cgggggagag
     9901 tcaccaggcc ccgcccgcag catccggcgc gattccggcg gggcccttgc ccgctggtcc
     9961 gatcgatagc gggccttcgt gccggtctgg tgggtggagg tgggtcggtg tctcccggtc
    10021 cacctctttt tttttcgtgc ccgccaaaaa aaaccggtcg acaccccgaa gcgtcgaccg
    10081 gctcgcaatg cccaatcgat gaaattggac tagcccgaaa ctggaaaggc tcgttcaccc
    10141 gtgcgcgtcc gccttcagca gcacgccgat gttggggttg agctcgtcga agatcggcag
    10201 cgccgcggcg cccagggcgg ccgtgtcgtg gccggccagg ccggcgatga cgcgcggcgt
    10261 gcggcggttg cggcgcgcag cgaccgaggt gtacagcggc tccaggcgtt cgatgagcag
    10321 cgcgagcacc gacgacggca ggaagccgcc gatcacgacc gcctcggcat cgagcagcga
    10381 ctccaggatg ttgaccgcct ggcgcaggcg ctgcgcggcc tcgtcgagcc acgcgtccag
    10441 gcgcgcgcgc gcctgcgggc tcgggtcgag cagcatctgc ggcgtggcgt gggcgacgtc
    10501 gccgagctcc agcgcctcgt acgcggcctg cagcgagacg tagcgctcga ggcagccgcg
    10561 gttgccgcag gggcaggcgc ggccgtcggg cacgacgatc atgtgcccca cctcgccggc
    10621 gttgtggccc gagcccttgt acaggtggcc gtcgtggaac atgcccgcgc ccaggccggt
    10681 gccgatgaac aggtaggtga agctgcggaa ggtgcgcgcg acgccgtgca gccgctcgcc
    10741 gatcgcggcg gcggtggcat cgttctccat gatcacgggc aggccgatcg cgttgcccac
    10801 ctgttgcgcg atcgagagat cctgccaccc cggcagcgcg gtcggcccga cggccgacag
    10861 gccctcgacg tcgaacggcc cgggcaccgc cagtcccagg cccaggacac gctcccagtc
    10921 ggcgtcggag gacttgcgca gcgtctcgac gacgtcgcgc accagcggca gcgcctgcgc
    10981 gggcgtcgga tggtcgaccg ccagcgtgca gcgcccgcgc accttgccgg agagatccac
    11041 gaccacggcg agcatggtgt tctggtcgat ctgcaggccg atcgagtagg cgccgtccgg
    11101 attgatcacg aacggcgtcg ccggctgccc gcgggtgccg cgctcgggcg tgagcgcggt
    11161 caggaatccg agcttgagga gctcgctggc gatgttggag atggtctgcg gcgtgagcgc
    11221 ggtcaggcgt gcgatgtcgg cgcgcgtgag gcggccgtgc aggcggatcg cctccatcac
    11281 gacgcgccgg ttgtgcgaac gcgcgaattc gaggttggtc ccggacaggc tgacggcagg
    11341 aggcttgttc accgaggtca tgtggagagc gctgcggcgc agtgtattaa cttaatcaaa
    11401 ttgaagtaat gctcgacaac ccgcatcgcc ggtctccggc gaacgttcct cggcgagtcc
    11461 gggggctcga ccggggaagt gccgaaagct cgtcgtgacc gtcgatcgag ccggcggcgc
    11521 gagcgatctt cggcggacct gggcctggcg cgggacaagc accgctacgc gcccggcccg
    11581 acacgtcgcc aaccgcgggg ccatggcggc gagggcgtcc gcgcggggcg cgccgacgag
    11641 ccggattgtc gctcgcgccg tccgatccgg ctcgacgcgg cacgtctcgt cctgcgcgat
    11701 ccgcgcggga ccggggtggg tgtaaacccc ggtcggcccg cccttgaccc gaccggctcg
    11761 acttttgata atgcaaacaa attgatttaa cgacatctgc aactcatgcg tcagcgtcac
    11821 ggattccagt cgcgactgcg cgccgccttg ggcgtcgcgg cgcgcggact cgccgccgtc
    11881 gccttcgcgc tggcatgcga cgcggcggcg gccaagcccg tcgagctgag catgtgggtg
    11941 atgcccgtca cgctgaacgc gcagcatgac gtgcccgcgc tgctgcagcc gtggctggcc
    12001 aggcatccgg gcgtgaccgt gcgcgtgacg gtgctcgact gggaatccgg ctggaacaag
    12061 atcaccgccg ccgccgcgtc gcgccgcggg ccggacctgc tggagctcgg caccacctgg
    12121 atgcccgcga tcgcggcgat gggcgggctc gagcagctca ccgacgcgca gctggccgag
    12181 gtcgaccacg gccgcgccta cttccccgag ctgtggaaga cgacgcaggt gttcggcaag
    12241 cccggcgtct acggtctgcc gtggtacgcg gacgtgcgcg ccgcctggta ccgcaccgac
    12301 gcgttcgcgc aggcgggcgt cgacccgaag caggccttcg cgacctggga ttcgttccgg
    12361 gccgcgctgc gcaaggtcaa cggccaggtc gtcgcgggcc ggcgcgtcgc ggcgctcgcc
    12421 tacgcgggca agaacgactg ggacgtcgtg cacaacctcg cgccgtggat ctggaacgcc
    12481 gggggcgacc tgctcacgcc cgacaagcgc cattccgcgc tcgaccagcc cgccgcgctg
    12541 aaggcgatcg acttctactc ccgcctcgcg gtcgacggcc tcgtgccgcc cgacgcgctg
    12601 gagaaggact ccgacatcct cgaaggcgcc ttcgtggccg gcgactacgc ggtgatcttc
    12661 tcggggccgt ggctgctgcg gcgcatcctc gccgcgcccg cggacgcgcc cgtgcgcacg
    12721 cacttcgacg tcgcgcccta tccggccggg cccgccgggc gcgcgacgat cttcagcggc
    12781 tccaacctcg cgatcttcaa gggctcgcgc cacaaggccg aggcctggga cctgctgaag
    12841 tacctgggcg acaagccggc gcaggtggag ctcgcacggc tcgcgtcgat gatgcccacg
    12901 cggctggacg cggcgatgga tccctcgctg caggcgcgcc ccgcgttcgc caagatcacc
    12961 gcgatcgcgc tcgacggccg cgcctatccg ccgatcccgg cgtgggggcc gctcgaggcc
    13021 gtctacacca agcacctggg ccagctcgcg gagctgacct cgggcatctc gggcacctac
    13081 agcgtcgcgg cgatgaaggc cgagatcgcg cgcaccgtca aggaggccga caaggtgctg
    13141 gaggaagcga attgagcgcc gcatccgcgc ggcggcagga gcgcaggccc cggcgcttcg
    13201 atgcgttccc gtacctgctg ctcgccccgg cggcgctgat gatgctggtc gtcaacctcg
    13261 tgcccgtcgt ccagggcttg cggatgtcgc tgctgaagct caaccagtac acgctcagcc
    13321 agtacatgga cgcgcctttc gccggcctgc gcaactaccg cggcgtgctg ctcgatcccg
    13381 acgatcccgt gcatgccggc atgctgctcg cgctgcgcaa cacggcgctc tacgcgctgc
    13441 tggtgaccgc gtgctccgtc gggctcgggc tgggcgtggc gatgctgctc aatcgcgagt
    13501 tcgcgggcag gtcgctcgcg cgcaccctgc tgatgctgcc ctgggtcgtc ccgacctacg
    13561 tcgtcggcac gctgtggggc ttcatgtggc agcgcgacga gggcatcgtc aaccacgtcc
    13621 tcgtcgacgt gctgcacctg atggaccaca agccgttctg gctcatcggc ggcaacacgt
    13681 tcttcgcgat cgtcatcccc acggtgtggc gggcgtggcc ggtgctgatg ctgatcttcc
    13741 tcgccgcgct gcaggccgtg cccgaggagc tctacgaagc cgcgcgcctg gacggcgcca
    13801 acgcctggcg gcgcttcacg cacgtgacgc tgcccgcgct caagccggtg atcgtcatcc
    13861 aggtgatgtt ccagatcgtc gacaacgtct actcgtacaa catcgtcgcg atgatgttcg
    13921 gcaagggcgc ggggtatgcg ggcgagtggg gcgacctgct gatgccgctg ctcacgcgcc
    13981 agagcttcag ctactggcgc ttcggcgagg gcgccgccgt ctcgttcgtg atgatggcgc
    14041 tgatgctcgt gttcgtcggc gtctggatgc gcgcgttccg caggagcatg accagtgaag
    14101 gctgacgcgc cgcccctgct cgcgcccgcg ccggcagcgt cggcgcaagg cgtcgcg
//
LOCUS       DASVLN010000269         4267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_444489, whole genome shotgun sequence.
ACCESSION   DASVLN010000269 DASVLN010000000
VERSION     DASVLN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444489"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF453_06135"
     CDS             <1..502
                     /locus_tag="VF453_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX7687263.1"
                     /translation="AMAEYPRPGHDDQTDFYIAERKPGAVLRPYRMGDPPAITVRAGT
                     TEEWVIENWTNELHTFHIHQVHFRVLDVDGEPVADPSLRDVVEVPYATATGYRSKEGP
                     VRPGRVRVKMTFPAELAGDIPFHCHLVDHEDNGMMAVIRVLPPGARRPPRTAGAGAMA
                     GMRMDH"
     gene            complement(496..1008)
                     /locus_tag="VF453_06140"
     CDS             complement(496..1008)
                     /locus_tag="VF453_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687264.1"
                     /translation="MPRILTVDRKLFSWWRQVHRVLDERGEPLYEMTWRWGWPTRTWV
                     VSRGGRPVAAMRRKLLAFAPTWIVEAAGARFLWRRKLLALRRRTQAIGGPFDGAELTG
                     SLLGLSFRLERGGRLVARARERILTLRERHDVELLDDTPDGELLAALAMFNLLLQKEA
                     EQEERAATGQ"
     gene            1294..1881
                     /locus_tag="VF453_06145"
     CDS             1294..1881
                     /locus_tag="VF453_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7687265.1"
                     /translation="MSHRPARAALCAAAVLLAALAAPAHATDVALPGDGTWLEFDVDS
                     FAAPSFGTGWIDQGDGSPLSFSFTVAAGHTATLTVVDAGFAGDTFAVTNHGAALGATS
                     AVPAGTADGDVVFDFDAALADPAYSRGTWTLGPGSYAIGGSLLQSVTMDGAALDATDG
                     GIRLATSVPEPALPALLVAGLAVVGAAARRRKNAA"
     gene            1900..3834
                     /locus_tag="VF453_06150"
     CDS             1900..3834
                     /locus_tag="VF453_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEX7687266.1"
                     /translation="MNAKRTLLAAACSLAAAAASCGTATAATAAPAPITIKIVGFNDF
                     HGNLQSPGTFGQNTVVPPSQRPAVGGVDYIGAYVARLKAQNPLNVVVGAGDIIGASPL
                     VSALFFDEPAVETMNHIGLEFTSVGNHEFDKGSAELRRLQNGGCHTTGGVPDANSCKG
                     LGSRAPGTFDGAKYRWLSANVIENATGRPLLAPYGIKSFNGVKVAFIGMTLKATPTIV
                     TPTGVAGLTFLDEADTVNALVPRLRAQGIEAIVVLVHQGGFQSSPNVGDINGCDGNLK
                     NADGSDSDIANIVKRLDNAVDLVISGHTHAAYNCSANTVDVKNVGGKAVSTPRPTGLP
                     NKVGRLVPVTSASAFGRIVTDIDMTIDPKTHDVIAVSPTNRLVDRTDPAAQAAVAADP
                     TVANIVAGYNTLVSPLANAVIGTITTALPSTANAAGEEPAGDVIADAQLQATQPASLG
                     GAQMAFMNAGGVRSPGFLSPSNTYPYDLTYGNAFTVQPFGNSLVTMTVTAQQLKNLLE
                     QQFSGCMGQTANRVMQSSNGLAYSWSASAPACSKIVDVTYTPTDVTVTPPVVTGAPDR
                     IVVGGVVQNPAKTYRITVNNFMATGGDGFTALLGGQNSLGGAQDIDALTAYLANYKAP
                     HAAYDPTQPALAIPRVTLLP"
     gene            3903..>4267
                     /locus_tag="VF453_06155"
     CDS             3903..>4267
                     /locus_tag="VF453_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687267.1"
                     /translation="MRRRRASVRLAAAAALAALLCTGSSALAHDDDPPSPARAALVAH
                     AEDELARGHAAAAMDDFERAGQMQHTPDAEMGLIRAELQDGQYRRALAFCAHTAGEHL
                     EAADGGALYAWLLRMGGQDA"
BASE COUNT          617 a         1664 c         1454 g          532 t
ORIGIN      
        1 cgcgatggcc gaataccctc gccccggcca tgacgaccag accgacttct acatcgccga
       61 acgcaagccg ggcgccgtgc tgcggccgta caggatgggc gatccgcccg cgatcaccgt
      121 gcgggcgggc accaccgagg agtgggtgat cgagaactgg accaacgagc tgcacacctt
      181 ccacatccac caggtccact tccgcgtgct cgacgtcgac ggcgagccgg tcgccgaccc
      241 gtcgctgcgc gacgtcgtcg aggtgcccta cgcgaccgcc accggctacc gcagcaagga
      301 agggccggtg cggccgggcc gcgtgcgcgt gaagatgacg ttcccggcgg agctcgcggg
      361 cgacatcccg ttccactgcc atctcgtcga ccacgaggac aacggcatga tggccgtgat
      421 ccgcgtgctg ccgccggggg cgaggcgccc gccgcgcact gccggcgccg gcgcgatggc
      481 cggcatgcgg atggatcact gaccggtcgc ggcccgctcc tcctgctccg cttccttctg
      541 cagcagcagg ttgaacatcg ccagggcggc gagcagctcg ccgtcgggcg tgtcgtcgag
      601 gagctcgacg tcgtggcgct cgcgcagcgt gaggatgcgc tcgcgcgccc gcgcgacgag
      661 gcggccgccg cgctcgaggc ggaagctcag gcccagcagg ctgcccgtga gctcggcgcc
      721 gtcgaacggg ccgccgatcg cctgcgtccg ccggcgcagc gcgagcagct tgcggcgcca
      781 caggaagcgc gcgccggcgg cctcgacgat ccaggtcggc gcgaacgcga gcagcttgcg
      841 ccgcatcgcc gcgaccggcc ggccgccgcg gctcacgacc cacgtgcgcg tcggccagcc
      901 ccagcgccac gtcatctcgt agagcggctc gccgcgctcg tcgagcacgc ggtgcacctg
      961 cctccaccac gagaagagct tgcggtcgac ggtgaggatg cggggcatgg cgggactggg
     1021 cgacgaggtg gcgggcagta tgcgcccagg cggcggtccg gccgggcgtt cgcggacgat
     1081 cgcccatgcc gtcgatcgac ccttcgctcg cgagcctcgc ctccgccgtg ctgctgcatc
     1141 aggcgctggc gcaggccccg gcgcagtgag cgccccggcg caaccgggac aactcctgcc
     1201 gcgaccccgg cgagggtcat cgcttcttca cgggctcgtc acgcgtcgca cggatagtcc
     1261 ggcgcactcc aacgaaatcc cgaggaagag accatgtccc accgtccggc ccgggccgcg
     1321 ctctgcgccg ccgccgtcct gctcgccgcg ctcgcggcgc ccgcgcacgc caccgacgtc
     1381 gccctgcccg gcgacggtac ctggctggaa ttcgacgtcg acagcttcgc cgcgccgtcc
     1441 ttcggcaccg gctggatcga ccagggcgac ggctcgccgc tgagcttctc gttcacggtc
     1501 gccgccggcc acacggcgac gctcaccgtg gtggacgccg gcttcgcggg ggacaccttc
     1561 gccgtgacca accatggcgc ggcgcttggc gccacgtcgg ccgtgcccgc cggcaccgcc
     1621 gacggcgacg tggtgttcga cttcgacgcc gcgctcgccg atcccgccta cagccgcggc
     1681 acctggacgc tcggcccggg cagctacgcc atcggcggca gcctgctgca gtcggtgacg
     1741 atggacggcg cggcgctgga cgcgaccgac ggcgggatcc gccttgcgac ctcggtgccc
     1801 gagccggcgc tgcccgcgct gctggtggcc ggcctggccg tcgtcggtgc cgccgcgcgc
     1861 cgccgcaaga acgccgcctg acgcgaagga gtccacgcca tgaacgccaa gcgcaccctc
     1921 ctcgccgccg cctgctcgct cgccgcggcc gccgcctcct gcggcaccgc caccgcggcc
     1981 accgcggccc cggcgccgat cacgatcaag atcgtcggct tcaacgactt ccacggcaac
     2041 ctgcagtcgc ccggcacctt cggccagaac accgtcgtgc cgccgtcgca gcggcccgcc
     2101 gtcggcgggg tcgactacat cggcgcctac gtcgcgcggc tgaaggcgca gaacccgctc
     2161 aacgtcgtcg tcggcgcggg cgacatcatc ggcgcgtcgc cgctggtctc ggccctgttc
     2221 ttcgacgaac ccgcggtcga gacgatgaac cacatcggcc tggagttcac ctcggtgggc
     2281 aaccacgagt tcgacaaggg ctcggccgag ctccggcgcc tgcagaacgg cggctgccac
     2341 acgacgggcg gcgtgcccga cgccaacagc tgcaagggcc tgggctcgcg cgcgccgggc
     2401 accttcgacg gcgcgaagta ccggtggctg tcggccaacg tgatcgagaa cgcgaccggc
     2461 cgcccgctgc tcgcgcccta cggcatcaag tcgttcaacg gcgtgaaggt cgccttcatc
     2521 ggcatgacgc tgaaggcgac gccgaccatc gtgacgccca ccggcgtcgc cggcctgacc
     2581 ttcctcgacg aggccgacac ggtcaacgcg ctcgtgccgc gcctgcgcgc ccagggcatc
     2641 gaggccatcg tcgtgctggt gcaccagggc ggcttccagt ccagcccgaa cgtcggcgac
     2701 atcaacggct gcgacggcaa cctgaagaac gccgacggca gcgactccga catcgccaac
     2761 atcgtcaagc gcctggacaa cgcggtcgat ctcgtcatca gcggccacac gcacgccgcc
     2821 tacaactgct cggccaacac ggtcgacgtg aagaacgtcg gcggcaaggc cgtctcgacg
     2881 ccgcgtccga ccggcctgcc caacaaggtc ggccgcctgg tgccggtcac cagcgcgagc
     2941 gccttcggcc gcatcgtcac cgacatcgac atgacgatcg acccgaagac gcacgacgtc
     3001 atcgcggtct cgccgaccaa ccgcctcgtc gaccgcaccg acccggccgc gcaggccgcg
     3061 gtcgccgccg acccgacggt ggccaacatc gtcgccggct acaacacgct cgtctcgccg
     3121 ctggccaatg ccgtgatcgg caccatcacg acggcgctgc cctcgaccgc caacgcggcc
     3181 ggcgaggagc ccgcgggcga cgtcatcgcc gacgcccagc tgcaggcgac gcagccggcg
     3241 agcctgggcg gcgcccagat ggcgttcatg aacgcgggcg gcgtgcgctc gccgggcttc
     3301 ctgtcgccgt cgaacaccta cccgtacgac ctgacctacg gcaacgcgtt cacggtgcag
     3361 cccttcggca acagcctggt gacgatgacg gtgaccgcgc agcagctcaa gaacctgctc
     3421 gagcagcagt tcagcggctg catgggccag accgccaacc gcgtgatgca gagctccaac
     3481 ggcctcgcgt actcgtggag cgcctcggcg cccgcgtgct cgaagatcgt cgacgtgacc
     3541 tacaccccga ccgacgtcac cgtgacgccg ccggtggtga cgggcgcgcc cgaccgcatc
     3601 gtcgtcggcg gcgtcgtgca gaacccggcg aagacctacc gcatcaccgt caacaacttc
     3661 atggcgacgg ggggcgacgg cttcacggcg ctgctgggcg gccagaactc gctcggcggc
     3721 gcgcaggaca tcgacgccct gaccgcctac ctcgccaact acaaggcccc gcacgcggcc
     3781 tacgatccga cgcagccggc gctcgcgatc ccgcgcgtga cgctgctgcc ttgagcgccg
     3841 cccggccgcg ctcgtcgcgc gctctgcggg gcggtcgctg ccgcgtcagc gcggcggcgg
     3901 ccatgcggcg ccgtcgcgcg tccgttcggc tggccgcggc ggccgcgctc gcggcgctgc
     3961 tgtgcacggg gtcgtccgcg ctggcgcacg acgacgaccc tccctcgccc gcacgcgccg
     4021 cgctcgtcgc ccacgccgag gacgagctcg cgcgcggcca cgccgccgcc gcgatggacg
     4081 acttcgaacg cgccggccag atgcagcata cgccggacgc ggagatgggc ctgatccgcg
     4141 ccgagttgca ggacggccag taccggcgcg cgctcgcctt ctgcgcccac acggcgggcg
     4201 agcacctcga ggccgcggac ggcggcgcgc tctacgcgtg gctgctgcgc atgggcggcc
     4261 aggacgc
//
LOCUS       DASVLN010000270         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_228263, whole genome shotgun sequence.
ACCESSION   DASVLN010000270 DASVLN010000000
VERSION     DASVLN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228263"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VF453_06160"
     CDS             complement(<1..1023)
                     /locus_tag="VF453_06160"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEX7687268.1"
                     /translation="MALTRLKSPAAAGRWLKSWVTGTLRTDSRALQPGDAFIAWPGYA
                     TDGRRFVASALASGAATCLVEEAGVEAFRFEDARVATLTNMKAAAGHIASAFFDEPSA
                     RLDVVATTGTNGKTSTAWWTAQALNLLGRRAGFVGTLGVGVPPLPQPVAAGDSKLDWT
                     IRPTGLTTPDPILLQATFRRMADDGFAACAMEASSIGIVEHRMAGTRVDVALFTNFTR
                     DHLDYHGSVDNYWAAKRLLFGWVGLKAASINVDDDAGALLADELARGPLDLWTYSVRP
                     EAGARLSAEGIGYRDGGLAFTLVERDAGGATHTAGVATTLIGEYNVSNLLAVLGGLRA
                     LGVPLAD"
     gene            complement(1037..2824)
                     /locus_tag="VF453_06165"
     CDS             complement(1037..2824)
                     /locus_tag="VF453_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7687269.1"
                     /translation="MIRKPGAPRTQASRGRAVSNVTYANSPLLASKTPLWRSRFLVVL
                     VGIGSLVLIGRAAVIQIISNDFYLKQGEARYAHTLEMPASRGRIIDRNGLLLAASVKA
                     PSIWAIPKDVDADTGERKQLAQALGMSVAELDERLDGNPNFVWLKRQVDDGVWRQVKA
                     LDLKGIHQVSEYRREYPQGEAAAHVVGFTGDEEKGQEGIELAYQQQLQGRDGSREVVK
                     DRLGNIVEDIGDPQRPVDGEDIELAIDSKVQFFAYQRIRDQVIAQKAKSGSVVVIDTK
                     TGEVLALANYPSYDPSDRHHLSGSQLRNRALTDVFEPGSTMKPFIVGTVLEKGLVTPY
                     SLVDATPGSLVIGGFAPKDAEPNGVLTVQQVIQKSSNIGAARLAMKLTPKEEWEKFSS
                     IGLGQKPQINFPGVVTGRLRPYKTWRPIEQATMAYGYGLSASLFQLARAYTVFANDGV
                     LENVSMLRQSSPGQGTRVFKPETVKEILTMLQMVASPGGTAPKARTLGYSVGGKSGTA
                     YKAEGHGYAQNKYRPWFVGLAPISHPRIVCAVMIDEPGAGLHYGGDVAAPVFSQVVSQ
                     TLPLLGVAPDLDVKTQVTMKDVKQVPESD"
BASE COUNT          377 a          996 c         1045 g          456 t
ORIGIN      
        1 gtcggccagc ggcacgccca gcgcgcgcag tccgccgagc acggcgagca ggttgctcac
       61 gttgtactcg ccgatcagcg tcgtcgccac gccggccgtg tgcgtcgcgc cgccggcgtc
      121 gcgctcgacc agcgtgaacg ccaggccgcc gtcgcggtag ccgatcccct cggcggacag
      181 ccgtgcaccg gcctcgggcc gcaccgagta cgtccacagg tcgagcggcc cgcgcgcgag
      241 ctcgtcggcg agcagcgcgc cggcatcgtc gtcgacgttg atcgacgcgg ccttgagccc
      301 gacccagccg aacagcagcc gcttggcggc ccagtagttg tcgacgctgc cgtggtagtc
      361 gaggtggtcg cgggtgaagt tcgtgaacag ggcgacgtcg acgcgcgtgc cggccatgcg
      421 gtgctcgacg atgccgatcg agctcgcctc catcgcgcac gcggcgaagc cgtcgtcggc
      481 catgcggcgg aaggtcgcct gcagcaggat cgggtcgggc gtcgtgaggc cggtgggccg
      541 gatcgtccag tcgagcttcg agtcgcccgc cgcgaccggc tgcggcagcg gcggcacgcc
      601 cacgcccagc gtgccgacga agccggcgcg gcggccgagc aggttcagcg cctgcgcggt
      661 ccaccaggcg gtcgaggtct tgccgttggt gccggtggtc gcgacgacgt ccaggcgcgc
      721 gctcggctcg tcgaagaacg cgctcgcgat gtggccggcc gcggccttca tgttggtcag
      781 cgtggccacg cgcgcgtcct cgaaccggaa cgcctcgacg ccggcctcct cgaccaggca
      841 ggtcgccgcg ccggaggcga gcgcggacgc gacgaagcgc cggccgtcgg tcgcatagcc
      901 cggccacgcg atgaacgcgt cgccgggctg cagggcgcgg ctgtccgtgc gcagcgtgcc
      961 ggtgacccag gacttgagcc agcggccggc ggcggcgggg gacttcaggc gcgtcaatgc
     1021 catgggatgc tcgtcctcaa tcgctttcgg gaacctgttt gacgtccttc atggtgacct
     1081 gcgtcttcac gtcgaggtcc ggcgccaccc ccagcagcgg cagcgtctgc gacaccacct
     1141 ggctgaagac gggcgccgcg acgtcgccgc catagtgcag gccggcgccc ggctcgtcga
     1201 tcatcaccgc gcagacgatg cgggggtggg agatcggcgc aagcccgacg aaccaggggc
     1261 ggtacttgtt ctgcgcgtag ccgtggccct cggccttgta cgcggtgccg gactttccgc
     1321 cgacgctgta gccgagcgta cgcgccttgg gcgccgtgcc gccgggcgac gcgaccatct
     1381 gcagcatcgt caggatctcc ttgaccgttt ccggcttgaa gacgcgcgtg ccctgcccgg
     1441 gactggactg ccgcagcatg gagacgttct cgagcacgcc gtcgttggcg aacacggtgt
     1501 aggcgcgcgc gagctggaac agcgatgccg acaggccgta gccgtaggcc atcgtggcct
     1561 gctcgatcgg ccgccaggtc ttgtacgggc gcaggcggcc ggtgacgacg ccggggaagt
     1621 tgatctgcgg cttctggccc aggccgatgg aggagaactt ctcccactcc tccttgggcg
     1681 tcagcttcat cgccaggcgc gcggcgccga tgttgctcga cttctggatg acctgttgca
     1741 ccgtcagcac gccattgggc tcggcgtcct tgggcgcgaa tccgccgatg accagcgagc
     1801 cgggcgtggc gtccaccagc gagtacggcg tgaccaggcc cttctcgagc acggtgccga
     1861 cgatgaaggg cttcatggtc gagccgggct cgaacacgtc cgtcagcgcg cggttgcgca
     1921 gctgcgagcc tgacaggtgg tggcggtcgc tcgggtcgta gctcgggtag ttggcgagcg
     1981 cgagcacctc gcccgtcttc gtgtcgatca cgaccacgct gccggacttg gccttctgcg
     2041 cgatgacctg gtcgcggatg cgctggtagg cgaagaactg caccttggag tcgatcgcga
     2101 gctcgatgtc ctcgccgtcg accgggcgtt gcgggtcgcc gatgtcctcg acgatgttgc
     2161 ccaggcggtc cttgaccacc tcgcgcgagc cgtcgcggcc ctgcagctgc tgctgatagg
     2221 cgagctcgat gccttcctgg cccttttcct cgtcgccggt gaagccgacg acgtgcgcgg
     2281 ccgcctcgcc ctgcgggtac tcccggcggt attcgctgac ctggtggatg cccttcaggt
     2341 cgagcgcctt gacctgccgc cacacgccgt cgtcgacctg gcgcttgagc cagacgaagt
     2401 tcgggttgcc gtcgaggcgc tcgtcgagct cggcgacgct catgcccagc gcctgcgcga
     2461 gctgcttgcg ctcgccggtg tcggcgtcga cgtccttggg gatggcccag atcgacggcg
     2521 ccttcacgct ggcggccagc agcaagccgt tgcggtcgat gatgcggccg cggctggccg
     2581 gcatctccag cgtgtgcgcg tagcgcgcct cgccctgctt caggtagaag tcgttgctga
     2641 tgatctggat caccgcggcg cggccgatca gcacgagcga gccgatgccc accagcacga
     2701 cgaggaagcg gctgcgccac agcggcgtct tggacgcgag cagcgggctg ttggcgtagg
     2761 tgacgttgct caccgcgcgg ccgcgggaag cctgcgtgcg cggggcgccg ggcttgcgga
     2821 tcatcgggac ccctccccgc tcgccggcgc ggagggccgg cgcgccgcgg gctc
//
LOCUS       DASVLN010000271         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_67453, whole genome shotgun sequence.
ACCESSION   DASVLN010000271 DASVLN010000000
VERSION     DASVLN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67453"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VF453_06170"
     CDS             complement(<1..160)
                     /locus_tag="VF453_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7687270.1"
                     /translation="MPSSASFDDLAAFVAVARAGGFRGAAVARGASASSLSEAVRRLE
                     ARLGVRLLH"
     gene            253..1143
                     /locus_tag="VF453_06175"
     CDS             253..1143
                     /locus_tag="VF453_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEX7687271.1"
                     /translation="MTVIDLAAAGTFALGHRIVKRIGYGAMQLAGPGVFGPPRDRAAA
                     LAVLREAVELGVDHIDTSDFYGPHVTNELIREALHPYRDGLTIVTKVGAKRGPQGSWE
                     PAFSAAELERAVHDNLRHLGLDVLDVVNLRLMFDVHGPAEGPIEAPLTALAELRAKGL
                     VRHLGLSNATRRQVAEARAIVDIACVQNQYNLAHRQDDAFIDELAAAGTAYVPFFPLG
                     GFTPLQSTALSAVARELGATPMQVALAWLLRRSPNLLLIPGTSTVAHLRENLAAASLQ
                     LPDEALAALDGIGAAAPAAH"
     gene            1227..1652
                     /locus_tag="VF453_06180"
     CDS             1227..1652
                     /locus_tag="VF453_06180"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7687272.1"
                     /translation="MTAPARWTRDYAHDIAADADVAWRTLADTTGWPAWNPGVLAVAL
                     EGPFASGSWFAMTLPDGMAIRTRLLEVEAPRRFVDETWIEGTVVRVDHRLEVAGPGRC
                     RVTFAVEAEGPQAAAMGEGASADFPEVLAGLARFVEARA"
     gene            1649..2110
                     /locus_tag="VF453_06185"
     CDS             1649..2110
                     /locus_tag="VF453_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7687273.1"
                     /translation="MTAKPLPTRHAGPGDSPGFLLWRVSNAWQREQRAALQAHGLTHT
                     QFVALAVATWFGHDGPLTQARLAKLTGSDPMTTSQVVRALEAAGHFRREAHRDDSRAK
                     AILVTPAGRKLAAAALPAVEAVDEAFFGGLAPAERQRLVKLFQRLAGPDEP"
BASE COUNT          322 a          914 c          834 g          301 t
ORIGIN      
        1 ggtggagcag ccgcacgccc agccgcgcct ccagccggcg cacggcctcg ctgaggctgg
       61 aggcgctggc gccgcgggcc acggcggcgc cgcggaagcc gccggcacgg gcgacggcga
      121 cgaacgccgc gagatcgtcg aacgacgcag aggagggcat tgttcgatta tccgcacggg
      181 ccgtccggat tgcgacggct agtcgaacgg ttgcgcgatt cctacagtgg cgccatcacc
      241 caaggagatt ccatgaccgt catcgacctc gccgccgccg gcaccttcgc cctcggccac
      301 cgcatcgtca agcgcatcgg ctacggcgcg atgcagctcg ccggccccgg cgtcttcggg
      361 ccgccccgcg accgcgccgc ggcgctggcc gtgctgcgcg aggccgtcga gctgggggtc
      421 gaccacatcg acaccagcga cttctacggc ccgcatgtca ccaacgagct catccgcgag
      481 gcgctgcacc cgtaccgcga cggcctgacg atcgtcacca aggtgggcgc gaagcgcggg
      541 ccgcagggct cgtgggagcc ggcgttctcg gccgcggaac tcgagcgcgc cgtccacgac
      601 aacctgcggc acctcggcct ggacgtgctc gacgtcgtca acctgcgcct gatgttcgac
      661 gtccacggcc cggccgaggg cccgatcgag gcgccgctga cggccctggc cgaactgcgc
      721 gccaagggcc tggtccgcca cctcggcctc agcaacgcga cgcgccggca ggtggccgag
      781 gcgcgcgcca tcgtggacat cgcctgcgtg cagaaccagt acaacctcgc ccatcgccag
      841 gacgacgcct tcatcgacga gctcgccgcc gccggcaccg cctacgtccc gttcttcccg
      901 ctgggcggat tcacgccgct gcaatcgacc gcgctgtcgg cggtcgcgcg cgagctgggg
      961 gcgacgccga tgcaggtcgc gctcgcgtgg ctgctgcgac gctcgcccaa cctgctgctg
     1021 atccccggca cgtccacggt cgcgcacctg cgcgagaacc tcgccgccgc gtccctgcag
     1081 ctgccggacg aggcgctggc ggcgctcgac ggcatcggcg cagcggcgcc ggccgcgcac
     1141 tgattcgcgt tcgggcgggg cgcccttgac aataaaataa gtgcacataa catatgcgca
     1201 cttgctactt gagcctggag cccgacatga ccgcccccgc ccgctggacg cgcgactacg
     1261 cccacgacat cgccgccgac gccgacgtcg cctggcgcac gctcgccgac acgacgggct
     1321 ggcccgcgtg gaacccgggc gtgctcgcgg tcgcgctgga gggcccgttc gcgagcggct
     1381 cctggttcgc gatgacgctg cccgacggca tggcgatccg cacccggctg ctggaggtcg
     1441 aggcgccgcg tcgcttcgtc gacgagacct ggatcgaggg cacggtggtg cgcgtcgacc
     1501 atcgcctgga ggtcgcgggc cccggccgct gccgcgtcac gttcgcggtg gaagccgaag
     1561 ggccgcaggc cgccgcgatg ggcgagggcg cctccgcgga cttccccgag gtgctggcgg
     1621 gcctcgcccg cttcgtcgag gcccgcgcgt gacggccaag ccgctgccga cgcggcacgc
     1681 agggcccggc gacagcccgg gcttcctgct ctggcgcgtc tccaacgcct ggcagcgcga
     1741 gcagcgcgcc gcgctccagg cgcacggcct cacgcacacg cagttcgtcg cgctggcggt
     1801 ggcgacctgg ttcggccacg acggtccgtt gacgcaggcg cggctcgcga agctgaccgg
     1861 cagcgatccg atgacgactt cccaggtcgt gcgcgcgctc gaggccgcgg gccacttccg
     1921 ccgcgaggcg caccgcgacg acagccgcgc caaggcgatc ctcgtgacgc ccgccggccg
     1981 caagctcgcc gccgccgcgc tgccggcggt cgaggcggtc gacgaggcgt tcttcggcgg
     2041 cctcgcccca gccgagcgcc agcgcctggt caagctgttc cagcggctcg ccgggccgga
     2101 cgaaccctga agcctgcgcc gctgacggag gagtcgcgaa gctgtcgcgg cgctgtccca
     2161 tgggtggcgt cccggatacc cgctggcgcc cggacctgcg acgctcgcgc cttcgccgaa
     2221 acggatccgc gtctaccaga cgacccttcc catgatcgag acgaattccc cgacggccgc
     2281 cgcggacgcc cacgaggccg ccggccggct gctgcagcag ggcatcgcgg ctgccggcga
     2341 cggccgcgcc gaggacgcga tcgcgcgttt t
//
LOCUS       DASVLN010000272         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_175952, whole genome shotgun sequence.
ACCESSION   DASVLN010000272 DASVLN010000000
VERSION     DASVLN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175952"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..367
                     /locus_tag="VF453_06190"
     CDS             200..367
                     /locus_tag="VF453_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX7687274.1"
                     /translation="MLRWALVFAIIALVAGLFGFTGIEAGAAEIARVLFFLFVVLVVV
                     FLVLGSNIFRR"
     gene            complement(410..1069)
                     /gene="sctR"
                     /locus_tag="VF453_06195"
     CDS             complement(410..1069)
                     /gene="sctR"
                     /locus_tag="VF453_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HEX7687275.1"
                     /translation="MNGSLPDPLTLVALIVAFGLAPFAALMVTCYTKLVIVFGLLRTA
                     LGLQQTPPNMVLNGIAIILSVYIMAPIGMDVADSLRNHAFGQKGEAVTDIMAVADAAK
                     QPLKDFLQKHTHERERRFFMDSATSVWPKARAQALRSDDFIVLVPSFTLSELTRAFQI
                     GFIIYMVFVVVDLIVATILLALGMSMISPTTISLPFKLLLFVMLDGWARLIHGLVLSY
                     R"
     gene            complement(1104..>1500)
                     /locus_tag="VF453_06200"
     CDS             complement(1104..>1500)
                     /locus_tag="VF453_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX7687276.1"
                     /translation="VVVEAAPGGAAGPRFIALAEGSRITIQQAREQTMNRPPEAPTAA
                     APRDAAELAVDRLDAIEVTLRFEVGDLAISLGDLRNVRAGHVFELGQPINRGPVRIVA
                     HGNLLGKGTLVAVGDRLGVRVSEFAPGEL"
BASE COUNT          213 a          510 c          549 g          228 t
ORIGIN      
        1 gctcgtcgac gcgggtcgcg tgcggccgca cgtcagcgcg gtctccccgc tgcgcgacgc
       61 gcgctcggca ctgcaggcga tcgaggaagg gcacacgctg ggcaaggcgg tcctggtgat
      121 ggatcgcgcg tgacgggtgc ctgcccggcg gcacggcgct tgccgtgtcc gccgcgatcc
      181 cacttccgaa agacgcatca tgctcagatg ggccctggtc ttcgccatca tcgcgctcgt
      241 cgccggtctc ttcggcttca ccggcatcga ggccggcgcc gcggagatcg cgcgcgtgct
      301 gttcttcctg ttcgtcgtcc tggtcgtcgt cttcctggtg ctcgggtcga acatcttcag
      361 gcgatagcgc ggccgggcgc cggccggtgc gcgccggccg gcgcccgcgt cagcggtagg
      421 acagcacgag tccgtggatc agccgcgccc agccgtccag catcacgaac agcagcagct
      481 tgaacggcag cgagatcgtc gtcggcgaga tcatcgacat gcccagcgcg agcaggatcg
      541 tcgcgacgat caggtcgacg acgacgaaca ccatgtagat gatgaagccg atctggaacg
      601 cgcgcgtcag ctcgctcagg gtgaagctcg gcacgagcac gatgaagtcg tcggagcgca
      661 gcgcctgcgc gcgcgccttg ggccacacgc tggtggccga gtccatgaag aagcggcgct
      721 cgcgctcgtg ggtgtgcttc tgcaggaagt ccttgagcgg ctgcttggcg gcgtcggcga
      781 ccgccatgat gtcggtgacg gcctcgccct tctggccgaa ggcgtggttg cgcagcgagt
      841 cggcgacgtc catgccgatc ggcgccatga tgtagaccga caggatgatc gcgatgccgt
      901 tgagcaccat gttcggcggc gtctgctgca ggccgagcgc ggtgcgcagc agcccgaaca
      961 cgatcacgag cttggtgtag cacgtgacca tcagcgccgc gaacggcgcg aggccgaacg
     1021 cgacgatcag cgcgacgagg gtcagcggat cgggcaggct gccgttcacg gggcgatcct
     1081 tccttgcggg gacgcgccgc ggctcacagc tcgcccggcg cgaactcgct cacgcgcacg
     1141 cccagccggt cgcccaccgc gaccagcgtc cccttgccca gcaggttgcc gtgcgcgacg
     1201 atgcgcaccg gcccgcggtt gatcggctgg ccgagctcga agacgtggcc cgcgcgcacg
     1261 ttgcgcaggt cgccgaggct gatcgcgagg tcgccgacct cgaagcgcag cgtgacctcg
     1321 atcgcgtcga ggcggtcgac ggcgagctcg gcggcgtcgc gcggcgcggc ggcggtcggg
     1381 gcttcgggtg ggcggttcat ggtctgctcc ctggcttgct ggatcgtgat gcgcgagccc
     1441 tcggccagcg cgatgaagcg gggccccgcg gcgcccccgg gcgcggcctc gacgacgacg
//
LOCUS       DASVLN010000273         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_14298, whole genome shotgun sequence.
ACCESSION   DASVLN010000273 DASVLN010000000
VERSION     DASVLN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14298"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VF453_06205"
     CDS             complement(<1..603)
                     /locus_tag="VF453_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687277.1"
                     /translation="MIRTLASSAALALAALAAAPAHAALALDTGTPGGQAIGALALDP
                     MDWYAAQVDFSKAGVVDAVSAHVLGGAPGETFHVSLYADDGFGRPGAALDTATVTFGT
                     DGWNGVAGLSGWTVQAGAYWIGIELADGDTLGSASATGALLDTGAPAPAQTAFSSFGS
                     TGYEAFGGLSFGARVDAVPEADSALTMLAGLALVAGLARKR"
     gene            complement(621..1868)
                     /locus_tag="VF453_06210"
     CDS             complement(621..1868)
                     /locus_tag="VF453_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7687278.1"
                     /translation="MPVSPRDARALARALATIAAAAAAALAPAAFAADTTPVVLTFST
                     VGDSRQDPNTYDQASVGATLDGQDATYLQNSKAFARILRTIQSQKSQLLFFNGDMING
                     YGWAAWGYTSNATQSAISGPVAPATTADIVGSDLMKHYREYAFWRGLVAGTMEQGVYV
                     FPVPGNHETECKACPPNADGQKQSKVENEQAYVASMGDLIVDSARFAGIVGSAPSNVQ
                     YGPAAGTGPDALSTDQTKLSYSFDWKNLHFAVINTDPVGQETSAPAQWLAADFAAASG
                     RGAKKFFVFGHKPAFTYAYAPGVSGSGLDATPTTTAKRNAFWNVIEQYHATYFCGHQH
                     TYHVSQPLGGAYQVIVGSGGSPFDPKASQATAGQLANPSTDRDYAWATVKVHADGGID
                     ILGYGFPANFGPTQLIGQYFIAP"
     gene            complement(2018..>2567)
                     /locus_tag="VF453_06215"
     CDS             complement(2018..>2567)
                     /locus_tag="VF453_06215"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX7687279.1"
                     /translation="PPPPDPVGDGVGEPHEALQSLVRGSAFQSTLLRLMAFAHGAHEG
                     VDDATVAGHDRPPLAALRRTLSRLLRRVLRDADGFADLPLPGRHKVRKRLKRLRYVAE
                     MLAPLHERDAVRAWTRVLGKAQDALGEHVDLALAAQRFERAAAAEPRQWFAVGWLRAE
                     SEATARAGRKALRRLREAEPFW"
BASE COUNT          338 a          892 c          982 g          355 t
ORIGIN      
        1 gcgcttgcgc gcgaggcccg cgaccagcgc cagtccggcc agcatcgtca gcgcgctgtc
       61 ggcctccggc accgcgtcca cgcgcgcgcc gaaggacagc ccgccgaacg cctcgtagcc
      121 ggtcgagccg aacgagctga aggccgtctg cgccggcgcc ggcgcgcccg tgtccagcag
      181 tgcgccggtc gcgctcgcgg agccgagcgt gtcgccgtcg gcgagctcga tgccgatcca
      241 gtacgcgccc gcctgcaccg tccagcccga gagcccggcg acgccgttcc agccgtcggt
      301 gccgaaggtg acggtcgcgg tgtcgagcgc ggcgcccggc cggccgaagc cgtcgtcggc
      361 gtagagcgag acgtggaagg tctcgcccgg cgcgccgccg aggacgtggg ccgagaccgc
      421 gtcgacgacg cccgccttcg agaaatccac ctgcgcggcg taccagtcca tcgggtcgag
      481 cgccagcgcg ccgatggcct ggcccccggg cgtgccggtg tcgagggcga gcgccgcgtg
      541 cgcgggcgcg gcggcgaggg cggcgagcgc gagcgccgcg gacgaggcga gggtgcggat
      601 catggacgtg tcccgtgcgc tcacggcgcg atgaagtact ggccgatcag ctgcgtcggc
      661 ccgaagttgg cggggaagcc gtagcccagg atgtcgatgc cgccgtcggc atgcaccttg
      721 accgtggccc aggcgtagtc gcggtcggtc gacggattgg cgagctggcc agccgtggcc
      781 tgcgatgcct tcggatcgaa cggcgagccg cccgagccga cgatgacctg gtacgcgccg
      841 cccagcggct gcgagacgtg gtaggtgtgc tggtggccgc agaaatacgt ggcgtggtat
      901 tgctcgatca cgttccagaa cgcgttgcgc ttggcggtcg tcgtcggcgt cgcgtccagg
      961 ccggagcccg agacgcccgg cgcataggcg tacgtgaacg cgggcttgtg gccgaagacg
     1021 aagaacttct tcgcgccgcg gcccgaggcg gccgcgaagt cggcggccag ccactgcgcg
     1081 ggggccgagg tctcctggcc gaccgggtcg gtgttgatca ccgcgaagtg caggttcttc
     1141 cagtcgaagc tgtaggagag cttggtctgg tccgtcgaca gcgcgtcggg gccggtgccc
     1201 gcggcggggc cgtactggac gttgctcggc gccgagccga cgatgcccgc gaagcgcgcg
     1261 ctgtcgacga tcaggtcgcc catgctcgcc acgtaggcct gctcgttctc gaccttggac
     1321 tgcttctgcc cgtcggcatt gggcgggcag gccttgcact cggtctcgtg attgcccggc
     1381 accgggaaca cgtagacgcc ctgctccatc gtgccggcca cgagcccgcg ccagaacgcg
     1441 tactcgcgat agtgcttcat caggtccgag cccacgatgt cggcggtcgt cgccggcgcc
     1501 accgggccgc tgatcgcgct ctgcgtggcg ttggaggtgt agccccacgc ggcccagccg
     1561 tagccgttga tcatgtcgcc gttgaagaac aggagctgcg acttctgcga ctggatcgtg
     1621 cgcaggatgc gcgcgaaggc cttgctgttc tgcaggtagg tggcgtcctg gccgtccagc
     1681 gtcgcgccga cgctcgcctg gtcgtaggtg ttgggatcct ggcgcgagtc gccgacggtc
     1741 gagaaggtca gcacgaccgg cgtcgtgtcg gcggcgaagg ccgcgggcgc gagggcggcg
     1801 gcggccgcgg ccgcgatcgt ggcgagtgcg cgcgcgagcg cgcgtgcgtc gcgaggggac
     1861 acgggcatgg ggagctcctt cacggtttcg ttgtcatcgt cgccggccac gggcttgccc
     1921 ggccggagcg caacgcactg tagggagcgg gaatgacggg cccgtgacgt ctagatgacg
     1981 cggcgccttc ggcctgcgcg gatcgcgaat cgacgcttca ccagaacggc tcggcctcgc
     2041 gcagccgccg cagcgccttg cggcccgcgc gcgcggtggc ctccgactcg gcgcgcagcc
     2101 agccgaccgc gaaccattgg cgcggctcgg cggcggcggc gcgctcgaag cgctgcgccg
     2161 ccagggcgag atcgacgtgc tcgccgagcg cgtcctgcgc cttgccgagc acgcgcgtcc
     2221 acgcgcgcac cgcatcgcgc tcgtgcagcg gtgcgagcat ctcggcgacg tagcggagcc
     2281 gcttcaggcg cttgcgcacc ttgtgccgcc cgggcagcgg caggtcggcg aagccgtcgg
     2341 cgtcgcgcag cacccggcgc agcaggcgcg aaagcgtgcg ccgcagcgcc gcaagcggcg
     2401 ggcggtcatg gccggcgacg gtggcatcgt cgacgccctc gtgcgcgccg tgcgcgaagg
     2461 ccatcagccg cagcagcgtc gactggaacg ccgacccgcg aaccagcgac tgcagtgcct
     2521 cgtgcggttc gccgacgccg tcgccgaccg gatcgggcgg cggcgga
//
LOCUS       DASVLN010000274        10875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_228517, whole genome shotgun sequence.
ACCESSION   DASVLN010000274 DASVLN010000000
VERSION     DASVLN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10875
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228517"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VF453_06220"
     CDS             <1..1078
                     /locus_tag="VF453_06220"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX7687280.1"
                     /translation="LAHPGEDAGAVYVLSNQASGNAVVVYGRDEAGRLRFERSVPTGG
                     RGTITQPPPNGAPGIDPLGSQGAIVYRDGLLLAVNAGSNDVSLFEADEHGGLRLLDRA
                     PSGGVEPVSLAVRGDIVLVANAGGTPNITSLRLDRHRARLEPIADGSTLLPGGTGAAP
                     GEVAVSPDGDTVVVTEKGTNLIDTFRLRDDGRSVLRASSASVGNTPFGFAIGRHGVVT
                     VVDAAAGAASSYAIEAGGALRTVTGALSLDGQLAPCWLVETHDGRLAFTGNAGTQGIS
                     ALSISGRGDLALLAAAAATTNGAALDLGLSPDDRFLYARSANGADAAAPGSVTGFRVH
                     RDGSLTAIDTVSDVPPGAQGIAVR"
     gene            complement(1105..2004)
                     /locus_tag="VF453_06225"
     CDS             complement(1105..2004)
                     /locus_tag="VF453_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7687281.1"
                     /translation="MDLLTDIALLMARHVSKDGFHATAIERLTLARSSTVTMPMPNVY
                     RPQLCFVAQGRKEVTLGRRLLAYAPGIYGIVTHDVPVTGQVVQASPDMPYLCMFLEFD
                     PVLLGQMALRVPAPSAAASLSIGKTVSPAGEGLLEAIVRLLRLLDEPSSQPVLAPLAE
                     QEILYRLLAGPDGARMRSVTAGQGRVAQIGRAIAWIGQHFRERLSVERLALEVGMSPS
                     SLHQHFRSVTSMTPLQFQKRLRLQDARSLMLMEDLDVTTTALRVGYESPSQFSREYRR
                     HFGEPPARDVARLRIARPPEVSA"
     gene            2171..3130
                     /locus_tag="VF453_06230"
     CDS             2171..3130
                     /locus_tag="VF453_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011547479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7687282.1"
                     /translation="MSIDRPRLPSVALGTWAWGDSGEAGDGYFGSRLTRAGLEELTEK
                     AHAAGFSLWDTAVVYGMGRAESVLGDLLRRYDRGSYQLSTKFTPQVAGGGEQPMGDML
                     AQSMARLGTDYIDLYWIHNPADVERWTPHLIPLLVSGKVRHVGVSNHNVREIERANQI
                     LGKAGFRVEAVQNHYSLLYRDAEEAGILDYCRDQDIPFFAYMVLEQGALGGRYGPGNP
                     MPEGTQRAKVYNGMLHQLKDLTATLGSIGQPVGATAAEVAAAWAIAKGTTPIVGVTRP
                     SHVEGLARARSMALSSEEVARLEALASGVEADTRGWWEKDLRA"
     gene            3287..4489
                     /locus_tag="VF453_06235"
     CDS             3287..4489
                     /locus_tag="VF453_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687283.1"
                     /translation="MTYLPTLSLVALGLASAAGPAGAGRMQIPELLPEHQEQLSARRL
                     RDARDDAARAEQLRLDAQRQQDALRRGTGDGGGGLPGGERHRVGNYRIDCLDCNFTGL
                     VLRPVKINSSGTVAANETTTGQGYVIAGGTLTAAPVVAGATFSTVSDINDHGHLAGVQ
                     AMAGSGNRGYLWRYGTINMIDGPNGGAASPRAINAADQVTGSAYGASGAYGAFLYRSG
                     ALTQLPLPPGHSYAWGTGINVAGHVVGSSYTGGTYADPDAWRYADGNLTVLSPQFVST
                     YANAVNDLDEVAGSVDSYAAYWSASGRLQILNTLPNFSTGTATAINVHGVVVGILAGN
                     NSAAWPRIAFMWDGRTVYDLNALIQGGTTSGWTLNEATSINDVGVIVGVGMLDGVTHA
                     FMATPLKR"
     gene            complement(4514..5932)
                     /gene="thrC"
                     /locus_tag="VF453_06240"
     CDS             complement(4514..5932)
                     /gene="thrC"
                     /locus_tag="VF453_06240"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7687284.1"
                     /translation="MKYISTRGDRTERSFSEILLEGLAPDGGLYLPTHYPQVDAATLA
                     RWRTLPYAELAFEILSLYVDDIPADDLRRLAAKTYTAEVFGTREIVPLRALEPGVMIE
                     ALSNGPTLAFKDMAMQLLGNLFEYELGRRGQTLNILGATSGDTGSAAEYAMRGKKGVS
                     VFMLSPNGRMSPFQQAQMFSLPDANIHNLCVEGVFDDCQDIVKAVSGDLAFKRRWRIG
                     TVNSINWARLLAQVVYYFAGWLQATKAGGEQVDFAVPSGNFGNVCAGHVARMMGLPIG
                     RLVVATNENDVLDEFFRTGTYRVRGAAETFETSSPSMDISKASNFERFVFDLLGRDGG
                     RTRALFAEGLARDGAFTLAADEKARIDRFGFVSGRSTHADRLATIRDTDARFGDVIDT
                     HTADGLKVAREHARPGTTMIVLETALPVKFAATIEEALGRKPQVPAELGDLESRPRRF
                     TVVPADVAVVKTFIETHAPAGS"
     gene            complement(5929..7248)
                     /locus_tag="VF453_06245"
     CDS             complement(5929..7248)
                     /locus_tag="VF453_06245"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX7687285.1"
                     /translation="MKPIQVGLLGIGTVGSGTYKVLTRNQEEIRRRAGRGIEIAMVAD
                     LDVERAKAVVGDGVRVVADARAVIADPAIDIVVELIGGYGVAKALVLEAIAAGKHVVT
                     ANKALLAVHGNEIFAAAREKGVMVAFEAAVAGGIPIIKALREGLTANRIEWIAGIING
                     TTNFILSEMRDKGLDFDVVLKEAQRLGYAEADPSFDIEGVDAAHKAAIMSAIAFGIPM
                     PFDKAHVEGIVGLQAADIKHAEALGYRIKLLGITRRRDDANGIELRVHPTLVPAKRLI
                     ANVEGAMNAVVVQADAVGTTLYYGKGAGSEPTASAVVADLVDVTRLHTADPEHRVPHL
                     AFRADALAATPILPIAQVVTSFYLRLCVADEAGVLSRITTTLAEHDISIDAVLQRPSP
                     DQKPGRQDLIILTHNTVEGRMRAAIEKMQALPTVLAPIVSLRKEDLA"
     gene            complement(7274..8302)
                     /locus_tag="VF453_06250"
     CDS             complement(7274..8302)
                     /locus_tag="VF453_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HEX7687286.1"
                     /translation="MTAASRAACAALLALAAGLARGQSFEHKDWQLACDNTRTCRAAG
                     YQADADEALPVSVLFTRAAGPATKVEGELQLGTFSDDSPHPGIVRVSIGGRAAGTVTL
                     DGDNHGALPPAVTEALLKALVGTGAITFSGGGSTWRLSGVGAAAVLLKMDDLQGRVGT
                     PSALVRKGAAPETNVKPPLPMPVVRAVRIPKAPRPGDDALAARIVATLHDVADCDLLG
                     DRTAPRDAGDAPALWHLDATRVLVTAPCWRAAYNTGDGYWVANAKPPYDPVLVTASGT
                     DFDEAAGISSQQKGRGIADCLGNEAWTWDGRGFVHTSETTSGMCRSIAGGGAWDLPTL
                     VTQVIPAK"
     gene            complement(8323..9549)
                     /locus_tag="VF453_06255"
     CDS             complement(8323..9549)
                     /locus_tag="VF453_06255"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7687287.1"
                     /translation="MKPIAKSSKLANIGYDIRGPVLDKARQMEDEGQKIIKLNIGNVA
                     AFGLEPPDEIVQDMIRNLPHAAAYTDSKGLFAPRRSVVHYTQEKRVAGVTIDDVYLGN
                     GASELIAMSMNALLDAGDEVLLPSPDYPLYTAVVGLSGGTPVHYVCDESSGWLPDLDD
                     IRRKVTPRTKAIVVINPNNPTGALYPVDVLEAIVEVARRHELIVFADEIYDKTLFDGE
                     QHTSIASLADDVLFVTFNGLSKNYRSCGYRAGWMVVSGAKRQARDYVEGLNMLASMRL
                     CANTPGQLAIQTALGGYQSIKDLVAPGGRLARQRDLAYELLTQIPGVSVVRPKAALYM
                     FPRLDPALYPIADDQQFAYELLAEEKVLIVQGTGFNWAATDHFRVVFLPNVDDLAEAI
                     GRIARFLDGYRKRHAA"
     gene            9836..10735
                     /locus_tag="VF453_06260"
     CDS             9836..10735
                     /locus_tag="VF453_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7687288.1"
                     /translation="MTRPATRAAGASSRRALLRGAALLALAANVAGTAFAQAKVLRVS
                     AIPDEAPTELQRKFTPLGEYLKAKTGMDVQFVPVSDYAAVVEGLADGKIDLAWLGGFT
                     YVQARIRTHGAVTPIVQRAEDAVFTSRIIVPLDSPIHTIAELKGHSFVFGSPSSTSGH
                     LEPRHFLMQQGIDPARDFKTIAFSGAHDATVAFVAAGRADAGALNASVMDKLAEAHNP
                     NVAKVRVLAVTPPFFDYNWTVRPGMDPALVKTLTDAFLALDPANPAQREIMALQRATR
                     FIPTKPSNYDGIEAAGRSAGLIK"
     gene            10732..>10875
                     /locus_tag="VF453_06265"
     CDS             10732..>10875
                     /locus_tag="VF453_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7687289.1"
                     /translation="MSVQLASASVVHANGRRAVDGVSLAIAQGERVALIGPSGAGKTT
                     LLRV"
BASE COUNT         1582 a         3804 c         3899 g         1590 t
ORIGIN      
        1 cctcgcccat cccggcgagg acgccggcgc ggtctacgtg ctgtccaacc aggcgtccgg
       61 caacgccgtc gtcgtctacg gccgcgacga ggccggccgc ctgcgtttcg agcgcagcgt
      121 ccccaccggc ggccgcggca ccatcacgca gccgccgccc aacggggcgc cgggcatcga
      181 cccgctcggc tcgcagggcg cgatcgtgta tcgcgacggg ctgctgctgg ccgtcaacgc
      241 gggcagcaac gacgtctcgc tgttcgaggc ggacgagcac ggcggcctgc gcctgctcga
      301 ccgcgcgccg tcgggcggcg tcgagccggt cagcctcgcg gtgcgcggcg acatcgtgct
      361 cgtcgccaat gccggcggca cgcccaacat cacgagcctg cgcctggatc gccaccgcgc
      421 ccgcctcgag ccgatcgccg acggcagcac gctgctgccc ggcggcaccg gcgcggcgcc
      481 gggcgaggtc gcggtcagcc cggacggcga cacggtcgtc gtgaccgaga aggggacgaa
      541 cctcatcgac accttccgcc tgcgcgacga cggccgctcg gtgctgcgcg cgtcgagcgc
      601 ctcggtgggc aacacgccct tcggcttcgc catcggccgc cacggcgtcg tgaccgtcgt
      661 cgacgcggcg gcgggcgcgg cgagctccta cgcgatcgag gccggcggcg ccctgcgcac
      721 ggtgaccggc gccctctcgc tcgacggcca gctggcgccg tgctggctgg tcgagacgca
      781 cgacggccgc ctcgccttca ccggcaacgc gggcacgcag gggatcagcg cgctgtcgat
      841 ctccgggcgc ggcgacctcg ccttgctggc cgcggcagcc gcgacgacga acggcgccgc
      901 gctggacctc ggcctgtctc ccgacgaccg cttcctgtat gcgcgcagcg ccaacggcgc
      961 cgacgcggcc gcgcccggct cggtcacggg tttccgggtg catcgcgacg gcagcctgac
     1021 ggccatcgac acggtctccg acgtgccccc gggcgcgcag ggcatcgccg tgcgctgagc
     1081 gtcggcgggc gtcatgtggc cccctcaggc ggaaacctcc gggggccgcg cgatacgcaa
     1141 gcgcgcgacg tcgcgcgcgg gaggctctcc aaagtggcga cgatattccc ggctgaattg
     1201 ggaaggactt tcgtagccga cgcgaagggc cgtggtggtc acgtcaagat cctccatcag
     1261 catgaggctg cgcgcgtcct ggagccggag ccgcttctgg aattgcaacg gcgtcatcga
     1321 ggtcacggat cgaaagtgct gatgcaggct ggaggggctc atgccgacct ccagagcgag
     1381 ccgctcgacg ctgagacgct cccggaagtg ctggccgatc caggcgatgg cacgaccgat
     1441 ctgggcgacc cgcccctggc cggccgtgac ggagcgcatc ctggcgccgt ccgggccggc
     1501 tagcaggcga tagaggatct cctgctctgc caggggtgcc aggacgggct gggaagatgg
     1561 ctcgtcgaga aggcgcagca ggcgaacgat cgcctcgagc aatccttccc cggcaggcga
     1621 taccgtcttg ccgatggaca aggacgccgc cgccgacggc gccggcaccc gcagcgccat
     1681 ctgtcccagg agtaccggat cgaactcgag gaacatacag agatagggca tgtccggcga
     1741 cgcctggacg acctggccgg tcacgggcac gtcatgggtg acgatcccgt agatgcccgg
     1801 ggcgtaggcg agcaagcgcc tgccgagcgt gacctccttg cgcccctgtg cgacgaagca
     1861 cagttgaggt cggtacacat tgggcatcgg catcgtgacc gtcgacgacc gggcgagcgt
     1921 cagtcgttcg atggcggtgg cgtggaagcc atccttggaa acatgccgcg ccatcaggag
     1981 cgcgatatcg gtcaacaggt ccacgggcgg actatcgcac gcagggcaag cgggcggggg
     2041 gcgggggccg aatccggagg atcgggcagt ccattcggtc gatcgtgctc acgctgcagc
     2101 ctgtgggcgc ggcacagtgt cgacggtgcc ggtgcgagac gcaccggctc gcaaccttca
     2161 aggaacgaac atgagcatcg atcgtccgcg cctgccgtcc gtggcgctgg gaacatgggc
     2221 ctggggcgac agcggggaag caggcgacgg ctacttcggc agccggttga cgcgagccgg
     2281 tctggaggaa ctgaccgaga aggctcacgc cgcgggattc agcctgtggg atacggcggt
     2341 ggtgtatggg atgggtcgcg cggaatcggt attgggagac ctgttgaggc ggtacgaccg
     2401 cggttcctac cagctctcca cgaagttcac gccgcaggtc gccggcggcg gcgagcagcc
     2461 gatgggcgac atgctggcac agagcatggc ccgcctcggt accgactaca tcgacctgta
     2521 ctggatccac aatccggcgg acgtcgagcg gtggacgcct cacctcattc ccctgctcgt
     2581 gagcggcaag gtccggcatg tgggcgtctc caaccacaac gtgcgtgaga tcgagcgggc
     2641 caaccagatc ctcggaaagg ccgggttccg ggtcgaagcc gtccagaacc actacagcct
     2701 cctctaccgc gacgccgagg aagcgggcat cctggactac tgccgcgacc aggacatacc
     2761 gttcttcgcc tacatggtgc tcgaacaggg agcgctcggc ggccggtatg gtcccggcaa
     2821 cccgatgccg gaaggcaccc agcgggcaaa ggtctacaac ggcatgctgc atcagttgaa
     2881 ggacctgacg gccacgctgg gctcgatcgg ccagccggtc ggagcgactg cagccgaggt
     2941 cgcggcggcg tgggccatcg ccaaggggac gacgcccatc gtcggcgtca cgcggccttc
     3001 gcacgtggag ggactggcgc gtgcgcggtc gatggcgctt tccagcgagg aggtcgctcg
     3061 actggaagcc ctggccagcg gcgtggaagc ggacactcgc gggtggtggg agaaggactt
     3121 gcgagcctga agcgcgcgcc ggcgccggat cgggcgccgt ggcgtcgtcg cgatggccag
     3181 cgcaattgcg cgcgatcaat cgggctccga aggccctcgg caggatttcg cacggacggc
     3241 cgcacgttgc cgccgccgac atccccaggc cttcaggagc acggacatga catacctgcc
     3301 caccctatcg ctcgtcgcac tcggcctggc gtccgccgcc ggccccgccg gcgccggccg
     3361 gatgcagatt ccggaactgc tgccggaaca ccaggaacag ctgtcggccc ggcggctgcg
     3421 cgatgcgcgc gacgacgccg cgcgcgccga gcagctccgg ctcgatgcgc agcgccagca
     3481 agatgccctg cggcgaggga cgggcgatgg cggcggcggc ctgcccggag gcgaacgaca
     3541 ccgtgtcggg aactaccgga tcgattgcct cgactgcaac ttcaccgggc tggtgctgcg
     3601 ccccgtcaag atcaacagct cggggaccgt ggccgccaac gagaccacga ccggccaggg
     3661 ctacgtgatc gccggcggca ccctcaccgc cgccccggta gtggccggcg ccaccttcag
     3721 cacggtgagc gacatcaacg accatggcca tctggcgggg gtgcaggcga tggccggatc
     3781 gggcaaccgg ggctatctct ggcgttacgg gacgatcaac atgatcgacg gcccgaacgg
     3841 gggcgccgcc tctccgcgcg cgatcaacgc ggccgatcag gtgacggggt ccgcctacgg
     3901 cgcttccggc gcctacggtg ccttcctcta tcggagcggc gctctgaccc agctgccatt
     3961 gccgcccggc cacagctacg cctggggcac gggcatcaac gtcgccggac acgtcgtggg
     4021 gagttcgtat acgggcggga cgtacgccga tcccgacgca tggcgatatg cggacggcaa
     4081 cctcacggtc ctctcgccgc agttcgtcag cacgtatgcc aacgcggtga acgacctgga
     4141 cgaggtggcc ggcagcgtcg acagctatgc ggcgtactgg agcgcctccg gccggctgca
     4201 gatcctgaac accctgccga atttcagcac cggcacggcc accgcgatca acgtgcacgg
     4261 cgtcgtggtg gggatcctgg ccggcaacaa cagcgccgcc tggccccgga tcgcgttcat
     4321 gtgggacggc cggacggtgt acgacctgaa cgcgttgatc cagggtggca cgacgtcggg
     4381 ctggacgctg aacgaggcca cgagcatcaa cgacgtcggc gtgatcgtgg gcgtcggcat
     4441 gctcgacggc gtgacgcacg ccttcatggc cacgcccctg aagcgctgat cgtcgaaggc
     4501 gccgcgcgcc ggcctaggag cctgccggcg cgtgcgtctc gatgaacgtc ttcacgaccg
     4561 ccacgtcggc cggcacgacc gtgaagcgcc gcggccgcga ctcgaggtcg ccgagctccg
     4621 cgggcacctg cggcttgcgg cccagggcct cctcgatcgt cgcggcgaac ttcaccggca
     4681 gcgcggtctc gagcacgatc atcgtcgtgc ccgggcgggc gtgctcgcgc gcgaccttca
     4741 ggccgtcggc ggtgtgggtg tcgatgacgt cgccgaagcg ggcgtcggtg tcgcggatcg
     4801 tggcgaggcg atcggcgtgc gtgctgcggc ccgagacgaa gccgaagcgg tcgatgcgcg
     4861 ccttctcgtc ggcagcgagc gtgaacgcgc cgtcgcgcgc cagcccctcg gcgaacagcg
     4921 cgcgcgtgcg cccgccgtcg cgaccgagca ggtcgaagac gaagcgctcg aagttggagg
     4981 ccttggagat gtccatcgac gggctcgagg tctcgaaggt ctcggccgcg ccgcgcaccc
     5041 ggtaggtgcc ggtgcggaag aactcgtcca ggacgtcgtt ctcgttggtc gccaccacga
     5101 ggcggccgat cggcaggccc atcatgcgcg cgacgtgtcc cgcgcagacg ttgccgaagt
     5161 tgcccgaggg cacggcgaag tcgacctgct cgccgccggc cttcgtcgcc tgcagccagc
     5221 cggcgaagta gtagacgacc tgcgcgagca ggcgcgccca gttgatcgag ttgaccgtgc
     5281 cgatgcgcca gcggcgcttg aacgcgaggt cgcccgagac ggccttgacg atgtcctggc
     5341 agtcgtcgaa cacgccctcc acgcacaggt tgtggatgtt ggcgtcgggc aggctgaaca
     5401 tctgcgcctg ctggaacggg ctcatgcggc cgttcgggct cagcatgaag acgctcacgc
     5461 ccttcttgcc gcgcatcgcg tattcggccg cgctgcccgt gtcgcccgag gtggcgccga
     5521 ggatgttgag cgtctgcccg cgccggccca gctcgtactc gaacaggttg ccgagcagct
     5581 gcatcgccat gtccttgaag gcgagcgtcg ggccgttgga cagcgcctcg atcatcacgc
     5641 ccggctccag cgcgcgcagc ggcacgatct cgcgcgtgcc gaagacctcg gcggtgtagg
     5701 tcttcgcggc caggcggcgc aggtcgtcgg cggggatgtc gtcgacgtag agcgacagga
     5761 tctcgaacgc gagctccgcg tacggcagcg tgcgccagcg cgcgagcgtc gcggcgtcga
     5821 cctgcgggta gtgcgtcggc aggtacaggc cgccgtcggg cgcgaggccc tcgagcagga
     5881 tctcgctgaa cgagcgctcg gtgcggtcgc cgcgggtgct gatgtacttc acgccaggtc
     5941 ttccttgcgc aggctgacga tgggcgccag aaccgtgggc agcgcctgca tcttctcgat
     6001 cgcggcgcgc atgcggcctt ccaccgtgtt gtgcgtcagg atgatgaggt cctggcggcc
     6061 cggcttctgg tcggggctcg ggcgctggag cacggcgtcg atggagatgt cgtgctcggc
     6121 cagcgtcgtc gtgatccgcg acagcacgcc cgcctcgtcg gccacgcaca ggcgcaggta
     6181 gaacgaggtg acgacctggg cgatgggcag gatgggcgtc gcggcgagcg catcggcgcg
     6241 gaaggcgagg tgcggcacgc ggtgctcggg gtcggcggtg tgcaggcgcg tcacgtcgac
     6301 gaggtcggcg acgacggccg aggcggtggg ctccgagccc gcgcccttgc cgtagtacag
     6361 cgtggtgccc acggcgtcgg cctgcacgac gacggcgttc atcgcgccct cgacgttggc
     6421 gatcagccgc ttggccggca ccagcgtcgg gtgcacgcgc agctcgatgc cgttcgcatc
     6481 gtcgcggcgg cgcgtgatgc ccagcaactt gatgcggtag ccgagcgcct cggcatgctt
     6541 gatgtccgcg gcctgcaggc cgacgatgcc ctcgacgtgc gccttgtcga agggcatcgg
     6601 gatgccgaac gcgatcgcgc tcatgatggc ggccttgtgc gcggcgtcca cgccctcgat
     6661 gtcgaagctc gggtcggcct cggcgtagcc caggcgctgc gcctccttga gcacgacgtc
     6721 gaagtcgagg cccttgtcgc gcatctcgga caggatgaag ttggtcgtgc cgttgatgat
     6781 gccggcgatc cactcgatgc gattggcggt caggccctcg cgcagcgcct tgatgatggg
     6841 gatgccgccg gccaccgcgg cctcgaaggc gaccatcacg cccttctcgc gcgcggcggc
     6901 gaagatctcg ttgccgtgca cggccagcag cgccttgttt gcggtgacca catgcttgcc
     6961 cgcggcgatc gcctccagca ccagcgcctt ggcgacgccg tacccgccga tcagctcgac
     7021 gacgatgtcg atcgccgggt cggcgatgac cgcgcgcgca tccgcgacca cgcgaacgcc
     7081 gtcgcccacg accgccttgg cacgctcgac gtcgaggtcg gccaccatcg cgatctcgat
     7141 gccgcggccc gcgcggcggc ggatctcctc ctggttgcgc gtcagcacct tgtacgtgcc
     7201 ggaacccacg gtcccgatgc ccagcaggcc gacttggatc ggtttcatct cgttgtcctc
     7261 tcgaaagatc tgttcacttc gccgggatca cctgcgtcac cagcgtcggc aggtcccacg
     7321 ccccgccgcc ggcgatgctg cggcacatgc cgctggtcgt ctcgctcgtg tgcacgaagc
     7381 cgcggccgtc ccaggtccag gcctcgttgc cgaggcagtc ggcgatgccg cgacccttct
     7441 gctgcgacga gatgcccgcg gcctcgtcga agtcggtgcc ggaggcggtc accagcacgg
     7501 gatcgtaggg cggcttcgcg ttggcgaccc agtagccgtc gcccgtgttg taggccgcgc
     7561 gccagcacgg cgccgtgacg agcacgcgcg tcgcgtcgag gtgccagagc gcgggcgcgt
     7621 cgccggcgtc gcggggcgcg gtgcggtcgc ccagcaggtc gcagtcggcg acgtcgtgca
     7681 gcgtcgcgac gatgcgcgcg gcgagcgcgt cgtcgcccgg gcgcggcgcc ttggggatgc
     7741 gcacggcgcg cacgaccggc atcggcagcg gcggcttgac gttcgtctcc ggcgccgcgc
     7801 ccttgcgcac cagcgccgac ggcgtgccga cgcggccctg caggtcgtcc atcttcagca
     7861 gcacggcggc cgcgcccacg cccgacaggc gccaggtcga gccaccgccc gagaacgtga
     7921 tggcgccggt gccgacgagc gccttgagca gcgcctcggt gaccgccggt ggcagcgcgc
     7981 cgtggttgtc gccatccagg gtcacggtgc ccgcggccct gccgccgatc gagacgcgca
     8041 cgatgcccgg atgcgggctg tcgtcgctga acgtgcccag ctgcagctcg ccctcgacct
     8101 tcgtggccgg cccggccgcg cgcgtgaaca gcaccgacac gggcagtgcc tcgtccgcgt
     8161 cggcctggta gccggcggcg cggcaggtgc gcgtgttgtc gcaggcgagc tgccagtcct
     8221 tgtgctcgaa ggactggccg cgcgccaggc cggcggccag cgccagcaag gccgcgcacg
     8281 cggcgcggga ggcggcggtc atggggcgtc gtctcccggg ctttaggcgg cgtgccgctt
     8341 gcggtagccg tccaggaagc gcgcgatgcg gccgatcgcc tccgcgaggt cgtcgacgtt
     8401 gggcaggaac acgacgcgga aatggtcggt cgcggcccag ttgaagccgg tgccctggac
     8461 gatcagcacc ttctcctcgg cgagcagctc gtaggcgaac tgctggtcgt cggcgatcgg
     8521 gtacagcgcc gggtcgaggc gcgggaacat gtagagcgcc gccttggggc ggaccacgct
     8581 cacgccgggg atctgcgtca gcagctcgta ggcgaggtcg cgctggcgcg cgaggcggcc
     8641 gccgggggcg acgaggtcct tgatgctctg gtagccgccg agcgccgtct ggatcgcgag
     8701 ctggcccggc gtgttcgcgc acaggcgcat cgacgcgagc atgttcagcc cctcgacgta
     8761 gtccctggcc tgccgcttcg cgcccgagac caccatccag cccgcgcggt agccgcagga
     8821 gcggtagttc ttcgacagcc cgttgaaggt cacgaacagc acgtcgtccg cgagcgaggc
     8881 gatgctcgtg tgctgctcgc cgtcgaacag cgtcttgtcg tagatctcgt cggcgaagac
     8941 gatcagctca tggcgacgcg cgacctcgac gatcgcctcg agcacgtcga ccgggtagag
     9001 cgcgccggtc gggttgttcg ggttgatgac cacgatcgcc ttggtgcgcg gcgtgacctt
     9061 gcggcggatg tcgtccaggt cgggcagcca gccgctcgac tcgtcgcaca cgtagtgcac
     9121 cggcgtgccg cccgacaggc cgaccaccgc ggtgtagagg gggtagtcgg gtgacggcag
     9181 cagcacctcg tcgcccgcgt ccagcagcgc gttcatgctc atcgcgatga gctcggaggc
     9241 gccgttgccg aggtagacgt cgtcgatcgt cacgccggcc acgcgcttct cctgcgtgta
     9301 gtgcacgacc gagcggcgcg gcgcgaacag gcccttggag tccgtgtagg ccgccgcgtg
     9361 cggcaggttg cggatcatgt cctgcacgat ctcgtccggc ggctcgagcc cgaacgcggc
     9421 gacgttgccg atgttgagct tgatgatctt ctggccctcg tcctccatct ggcgggcctt
     9481 gtcgaggacc gggccgcgga tgtcgtagcc gatgttggcg agcttgctgg acttggcgat
     9541 cggcttcacg gccggtggat cctgtcgggt ggagtggacg cggcacgcgg cttcgcggtg
     9601 gacgaagcgc ggcggggagt ccaagtgtct catgggcggc ccctggcacg gcaggtcgcc
     9661 gacccgcgcg ccggcccttt ttcgcatggc ccgcgacact tccccccgcg gcgccgcgtc
     9721 cgcacccgaa cgttgccttg accaacggcg aaggatggcc gcggcgccgg ccgttgaagg
     9781 caaaatgcgc ggccataccg gcgccgctct ccaggcgccg caacccggag cctccatgac
     9841 ccgtcccgcc acccgcgccg ccggcgcgtc gtcccgtcgc gccctgctgc gcggcgccgc
     9901 cctccttgcc ctcgcggcca atgtcgccgg caccgccttc gcgcaggcga aggtgctgcg
     9961 cgtctcggcc atcccggacg aggctccgac cgagctgcag cgcaagttca cgccgctggg
    10021 cgagtacctg aaggcgaaga ccggcatgga cgtgcagttc gtccccgtga gcgactacgc
    10081 cgcggtcgtc gagggcctgg ccgacggcaa gatcgacctc gcctggctgg gcggcttcac
    10141 ctacgtccag gcgcgcatcc gcacccacgg cgccgtcacg cccatcgtcc agcgcgccga
    10201 ggacgccgtc ttcacgagcc gcatcatcgt gccgctcgac agcccgatcc acacgatcgc
    10261 cgagctcaag ggccacagct tcgtcttcgg ctcgccctcg tcgacctcgg gccacctcga
    10321 gccgcgccac ttcctgatgc agcagggcat cgacccggcc agggacttca agacgatcgc
    10381 gttctccggc gcgcacgacg cgaccgtcgc cttcgtggcc gccggccgcg cggacgcggg
    10441 cgcgctcaac gcctcggtga tggacaagct ggccgaggcc cacaacccca acgtcgccaa
    10501 ggtgcgcgtg ctggcggtca cgccgccgtt cttcgactac aactggacgg tgcgccccgg
    10561 catggatccc gcgctggtga agacgctgac cgacgccttc ctcgcgctcg acccggccaa
    10621 cccggcgcag cgcgagatca tggcgctgca gcgcgccacg cgcttcatcc cgacgaagcc
    10681 gtccaactac gacggcatcg aggccgcggg gcgctcggca ggcctgatca agtgagcgtc
    10741 cagctcgcct cggcgagcgt cgtccatgcc aacggccggc gcgcggtcga cggcgtctcg
    10801 ctcgcgatcg cccagggcga gcgggtcgcg ctgatcgggc cgtcgggcgc cggcaagacc
    10861 acgctgctgc gcgtg
//
LOCUS       DASVLN010000275         3714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_336737, whole genome shotgun sequence.
ACCESSION   DASVLN010000275 DASVLN010000000
VERSION     DASVLN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336737"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1190)
                     /locus_tag="VF453_06270"
     CDS             complement(189..1190)
                     /locus_tag="VF453_06270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7687290.1"
                     /translation="MTLAQEAPPPPRRRLSLVTGSFNEEGNVDEWFERTFAACAGLAD
                     RYELEVIWIDNASADATVEKVRAWCARDRRVKLIVNARNFGVVRSPFHAMMQATGDAC
                     VYLASDLQDLPELIPDFVARWEQGFDVVAGVYRQSPDGFLMRRCRAAYYAILTRVSDS
                     DPVIRGFTGFGLFSRRVLEHMRHIGGPSPITRALVAEIGLPSTTVAYDKPPRKYGATS
                     NDVLRLIDQAVLALTSMSKAPVRIATIVGLALSAFGFVMAACYLIAKLLFWDSFPLGQ
                     APLLIGIFLFSSVQILIMGLIGEYVAAIHQRLQRHPWVVEKTRVNFDEDAPASRQDF"
     gene            1379..1780
                     /locus_tag="VF453_06275"
     CDS             1379..1780
                     /locus_tag="VF453_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX7687291.1"
                     /translation="MGLRRLATLLETHQFLRFLLAGGLNTLFGFGVYALSVLAGLPVW
                     LAMLVGTVAGIAFNFVTLGGYAFRDLSARRLPRFVLGYAGVYAVNLAAFDLVHRRVAN
                     PVACQVLLVVPVALFSYLVMSRLVFRKRREA"
     gene            complement(1786..2304)
                     /gene="moaC"
                     /locus_tag="VF453_06280"
     CDS             complement(1786..2304)
                     /gene="moaC"
                     /locus_tag="VF453_06280"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX7687292.1"
                     /translation="MTDSDDSASPPAAQATGLTHFDAAGRAHMVDVGAKPETRRVARA
                     AGSIRMQPATLAVIAAGSARKGDVLGVARIAAIQAAKRTAELVPLCHPLPLTHVAVEF
                     ALDEANGAVHCTVQAETLGRTGVEMEALTAVQVGLLTIYDMVKAIDRGMVMGDIRVLE
                     KHGGKSGDWSAA"
     gene            2448..3656
                     /locus_tag="VF453_06285"
     CDS             2448..3656
                     /locus_tag="VF453_06285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7687293.1"
                     /translation="MRLNLASLAFAACLALNAVVSTAPIPAQAQVRLPALGEAEEDSF
                     SITDEKRVGDAIMREIRPDPDVIDDPVLFEYLESIFRPLHDAARRRGDISDEVESTFA
                     WEPFLVRDRTFNAFALPGGYVGVNLGLIAVAGSRDEMAGVLGHELSHITQRHIARSMV
                     ANKHQSILTMAAMLIGLLASARAKNSDIPMAVLASGQAAQATGQLTFSRQMEAEADAF
                     GENVMTDAGFAPAGMAAMFERLATAEQLNDNGMYPWLRSHPMTLDRLATARLRAREVP
                     PPDPAKSHLTEHSLMRARARALMDTSDPALRRMQAQARLGAPLNDAERLGALYGGALA
                     SIELRDFATADTMLADARQVLAQHAALVAEQERERAAPPVSNPAASAADPAASGVHQA
                     VTVHGPLYPI"
BASE COUNT          526 a         1327 c         1320 g          541 t
ORIGIN      
        1 gaccggcaag acgctggcgc aggtcgccgt cgaccagtcc ccgatccacg tcgccctcct
       61 gccgcccaac tacgtcggac gcctcagcca gcccggcctg atcgcgctga tgcgcgagcc
      121 gagcccggtg aagcccttcg ccgcaccgaa cgtggcggga cgctagccgg ccgggctccg
      181 gcagccgctc agaaatcctg acggctggcg ggggcgtcct cgtcgaagtt gacccgggtc
      241 ttctcgacga cccacggatg gcgctgcagc cgctggtgga tcgcggcgac gtactcgccg
      301 atcaggccca tgatgaggat ctgcacggac gagaacagga agatgccgat cagcagcggc
      361 gcctggccga gcgggaacga gtcccagaac agcagtttgg cgatcagata gcaagcggcc
      421 atgacgaagc cgaatgccga cagcgcgagt cccacgatgg tggcgatccg caccggcgcc
      481 ttgctcatgg acgtcagcgc cagaaccgct tggtcgatca ggcgcagcac atcgttgctg
      541 gtcgcgccgt atttgcgcgg cggcttgtcg taggcgacgg ttgtcgacgg caggccgatc
      601 tccgcgacca gtgcgcgcgt gatggggctg gggccgccga tatggcgcat gtgctccagg
      661 acgcgacgtg agaacaggcc gaagcccgtg aagcctcgga tgacaggatc ggaatcgctg
      721 acacgggtca gaatcgcgta gtaggcggcg cggcaccggc gcatgaggaa gccgtccgga
      781 ctctggcggt agacacctgc gacgacgtcg aagccctgtt cccaccgcgc cacgaagtcg
      841 gggatcaact cgggtaggtc ctgcaggtcg ctcgcgagat aaacgcaggc atccccggta
      901 gcctgcatca tcgcgtggaa gggggagcgg acgacaccga aattgcgcgc gttcacgatg
      961 agcttgaccc gtcggtcgcg ggcgcaccag gcccgcacct tctcgaccgt ggcgtcggca
     1021 gatgcgttgt cgatccagat gacctcgagc tcgtagcggt ccgcgaggcc ggcgcacgcg
     1081 gcgaaggtgc gctcgaacca ctcgtcgacg ttgccctcct cgttgaagga gcccgtgacg
     1141 aggctgagcc tgcggcgagg cggcgggggg gcttcctggg cgagggtcat cctggcggtg
     1201 cgatgcgagc gggcaggccc gcattgtgag atatccggcg ggcgctgtcg tgcgaaggat
     1261 gtctcgcgcc ggccgtggcg cgtcgcccgg accgcggccg gcgcgcggat ccgggctatc
     1321 cttcgccgcg tccccgccac ctccaccgcc ggcccgagtc gagccgatcc ccacgcccat
     1381 gggcctgcgc cgcctcgcca ccctgctcga gacccaccag ttcctgcgct tcctgctcgc
     1441 gggcggactg aacacgctct tcggcttcgg cgtctacgcg ctctcggtgc ttgccggcct
     1501 gcccgtgtgg ctggcaatgc tcgtcggcac ggtcgcgggc atcgccttca acttcgtgac
     1561 gctgggcggc tacgcgttcc gcgacctgtc cgcgcggcgg ctgccgcgct tcgtgctcgg
     1621 ctacgcgggc gtgtacgccg tcaacctcgc ggcgttcgac ctcgtccatc ggcgggtggc
     1681 caatccggtg gcctgccagg tgctgctggt ggtgcccgtg gcgctgttct cgtacctggt
     1741 gatgtcgcgc ctcgtgttcc gcaagcggcg cgaggcctga cccgctcagg cggccgacca
     1801 gtcgccggac ttcccgccgt gcttctcgag cacccggatg tcgcccatca ccatgccgcg
     1861 gtcgatcgcc ttgaccatgt cgtagatcgt cagcaggccg acctgcacgg cggtcagggc
     1921 ctccatctcg acgccggtgc ggccgagcgt ctcggcctgg acggtgcaat gcacggcgcc
     1981 gttcgcctcg tccagcgcga actcgacggc cacgtgcgtc agcggcagcg gatggcacag
     2041 ggggacgagc tcggcggtgc gcttggcggc ctggatcgcc gcgatgcgcg cgacgcccag
     2101 cacgtcgccc ttgcgcgcgc tgccggcggc gatcacggcg agcgtcgcgg gctgcatgcg
     2161 gatcgagccg gcggcacggg cgacgcggcg cgtctcgggt ttggcgccga cgtcgaccat
     2221 gtgggcgcgg ccggcggcgt cgaagtgcgt gagcccggtg gcctgcgcgg cgggcgggct
     2281 cgcgctgtcg tcggagtcgg tcatggaacc tgggtggatc gggaaggtcg gatgcgggag
     2341 gcgccgctac aaggccgata catggcggtg tcatcatagc gaccgggcgc gccgccgcgg
     2401 cccgcgcccc gccgtccacg accgcacgcc ctgccggaaa gccgcccttg cgcctgaacc
     2461 tcgcctccct cgccttcgcc gcgtgcctcg cgctcaacgc cgtcgtgtcg accgcgccga
     2521 tcccggccca ggcccaggtg cgcctgccgg cgctcggcga ggccgaggag gactccttca
     2581 gcatcaccga cgagaagcgc gtcggcgacg cgatcatgcg cgagatccgc cccgaccccg
     2641 acgtcatcga cgacccggtg ttgttcgagt acctggagtc gatcttccgt ccgctgcacg
     2701 acgcggcgcg ccggcgcggg gacatcagcg acgaggtcga gagcacgttc gcatgggagc
     2761 ccttcctcgt gcgcgaccgc actttcaacg cgttcgcgct gcccgggggc tacgtcggcg
     2821 tgaacctcgg gctgatcgcc gtcgccggct cgcgcgacga gatggccggc gtgctcggcc
     2881 acgagctctc gcacatcacc cagcgccaca tcgcgcgcag catggtcgcc aacaagcacc
     2941 agagcattct cacgatggcg gcgatgctga tcggcctgct cgcttcggcg cgcgcgaaga
     3001 actccgacat cccgatggcc gtgctcgcga gcggccaggc ggcgcaggcg acggggcaac
     3061 tcaccttctc gcgccagatg gaggccgagg ccgacgcgtt cggcgagaac gtgatgaccg
     3121 acgcgggctt cgcgccggcc ggcatggcag cgatgttcga acgcctcgcg accgccgagc
     3181 agctcaacga caacggcatg tacccgtggc tgcgctcgca cccgatgacg ctggaccgcc
     3241 tggccacggc gcggctgcgc gcgcgcgagg tgcccccgcc cgatcccgcc aagagccact
     3301 tgaccgagca ctcgctcatg cgcgcccggg cgcgggcgtt gatggacacg tccgacccgg
     3361 cgctgcgccg catgcaggcg caggcgcggc tcggcgcgcc gttgaacgac gcggagcgcc
     3421 tgggcgccct ctacggcggc gcgttggcgt cgatcgagct gcgcgacttc gcgaccgccg
     3481 acacgatgct ggccgatgcc cgccaggtgc tggcgcagca cgcggcgctg gtcgccgagc
     3541 aggaacgcga gcgtgcggcg cctccggtct cgaatccggc ggcgagcgcc gccgacccgg
     3601 ccgccagcgg cgtccaccag gccgtcacgg tgcacgggcc gctgtacccg atctagccgg
     3661 aggtcgcgcg cgacttcgcg ctgctgcacc tggaggaggc gatcgcccgc cgcg
//
LOCUS       DASVLN010000276         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_228592, whole genome shotgun sequence.
ACCESSION   DASVLN010000276 DASVLN010000000
VERSION     DASVLN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228592"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VF453_06290"
     CDS             complement(<1..1452)
                     /locus_tag="VF453_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7687294.1"
                     /translation="MASTHDGPALGYDSDGATIQAHAFAYRRSPDQDAPVPARHPVVV
                     VGAGPVGLTLAIDLARRGVRVVLLDNDSRLSTGSRAICFAKRTLEIWDRLGVGDAMCA
                     KGVQWNTGRVFFRDAQLYQFDLLPETGHERPAFINLQQYYAEAFLAVRAGELPGIDLR
                     WKNTACGIEQRGDHARVAIDTPEGRYEIDADHVVACDGSRSPVRAMLGLESKGRVFKD
                     RFLIADVRMQADFPAERWFWFDPPFHPNRSVLLHRQPDGVWRIDFQLGWDADPEAEKQ
                     PERIRPRVDAMMRAALGREVPFELEWASVYTFSCLRMDAFRHGRVLFAGDAAHGVSPF
                     GARGANSGVQDADNLGWKLAAVLQGRAGDALLATYAPEREAAADENLLNSTRSTDFIT
                     PKSEASRLFRDAALALSRTQPFARALVNSGRLSTPATLATSPLNTPDAAGDSFESRMV
                     PGAPAVDAPVVHADGRRGWLLRALGPDFTLLLAD"
     gene            complement(1457..2416)
                     /locus_tag="VF453_06295"
     CDS             complement(1457..2416)
                     /locus_tag="VF453_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7687295.1"
                     /translation="MSTEKTFASQADLEEKKVTFSQLSEHAYAYTAEGDPNTGIIVGD
                     DAVLVADTQATPAMAADVIRRIREVTDKPIKYVVLTHYHAVRVLGASAYAPQEIIASR
                     DTYDLIVERGEADKASEIGRFPRLFRNVETVPAGLTWPTITFEQKMTLWLGKLEVQIL
                     QLGRGHTKGDTVVWLPQDRVLLSGDLVEFDATPYAGDAYFQDWPATLANIAALKPRAL
                     VPGRGAALMGEEQVARGLAMTRDFVSDLYASVKAGAAAGRELKAVYRETYDRLKPKYG
                     GWVIFDHCMPFDVTRAYDEATQYPDPRIWTAERDRQMWEALEG"
     gene            2583..>3100
                     /locus_tag="VF453_06300"
     CDS             2583..>3100
                     /locus_tag="VF453_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7687296.1"
                     /translation="MTRTKPEDEDKAQRAVQSVEVGGRLLLALAAHGAPMSLKDLAAG
                     AGLPSSRAHPYLVSFGKLGLVEQDGASGRYLLGPAALQLGLAALHQLDAVRSATPVAE
                     ALARETGHAVGLAVWGNFGPTVIRMIDASEPLHVAMRAGTVMSILGTATGRAFAAYLA
                     RERLERAMAGPLG"
BASE COUNT          412 a         1082 c         1161 g          445 t
ORIGIN      
        1 gtcggccagc agcagggtga agtccggccc gagcgcccgc agcagccagc cgcgccggcc
       61 gtccgcgtgc acgacgggcg cgtcgaccgc cggggcgccg ggcaccatgc ggctttcgaa
      121 ggaatcgccc gccgcgtccg gcgtgttcag cggcgacgtc gcgagcgtcg cgggcgtcga
      181 caagcgcccg ctgttgacca gcgcccgcgc gaagggctgc gtgcgcgaga gcgcgagcgc
      241 cgcgtcgcgg aacagccggc tcgcctcgct cttgggcgtg atgaagtcgg tcgagcgcgt
      301 cgagttgagc aggttctcgt ccgccgcggc ctcgcgctcg ggcgcgtagg tcgcgaggag
      361 cgcgtcgccg gcacggccct gcagcacggc ggcgagcttc cagcccaggt tgtccgcgtc
      421 ctgcacgccc gagttcgcgc cgcgcgcgcc gaagggcgag acgccgtgcg ccgcgtcgcc
      481 cgcgaacagc acgcgcccgt ggcggaacgc gtccatgcgc aggcacgaga acgtgtagac
      541 gctcgcccat tcgagctcga acggcacctc gcggcccagc gccgcgcgca tcatcgcgtc
      601 gacgcgcggc cggatgcgct cgggctgctt ctccgcctcg gggtcggcgt cccagccgag
      661 ctggaagtcg atgcgccaga cgccgtcggg ctggcggtgc agcagcacgc tccggttcgg
      721 gtggaagggc ggatcgaacc agaaccagcg ctcggcgggg aagtccgcct gcatgcgcac
      781 gtcggcgatc aggaagcgat ccttgaagac gcgccccttg ctctcgaggc ccagcatcgc
      841 gcgcaccggc gagcgcgagc cgtcgcacgc gacgacgtgg tccgcgtcga tctcgtagcg
      901 gccctcgggg gtgtcgatcg ccacgcgagc gtggtcgccg cgctgctcga taccgcaggc
      961 cgtgttcttc cagcgcaggt cgatgccggg aagctcgccc gcgcggacgg cgaggaaggc
     1021 ctcggcgtag tactgctgca ggttgatgaa ggcgggccgc tcgtggccgg tctcgggcag
     1081 caggtcgaac tggtagagct gcgcgtcgcg gaagaacacg cgcccggtgt tccactgcac
     1141 gcccttcgcg cacatcgcgt cgccgacgcc caggcgatcc cagatctcca gcgtgcgctt
     1201 ggcgaagcag atcgcgcgcg agccggtcga cagccgcgag tcgttgtcca gcagcacgac
     1261 gcgcaccccg cgccgggcca ggtcgatcgc gagcgtcagg ccgacgggcc cggcaccgac
     1321 cacgaccacc ggatggcgcg cgggcacggg cgcatcctgg tcgggcgagc ggcggtacgc
     1381 gaaggcgtgc gcctggatcg tcgcgccgtc ggagtcgtag cccagcgccg ggccgtcgtg
     1441 cgtcgacgcc atcgcgtcag ccctcgagcg cttcccacat ctggcgatcg cgctcggccg
     1501 tccagatgcg cgggtcgggg tactgcgtcg cctcgtcgta ggcgcgcgtg acgtcgaagg
     1561 gcatgcagtg gtcgaagatc acccagccgc cgtacttcgg cttgaggcgg tcgtaggtct
     1621 cgcggtagac cgccttgagc tccctgcccg ccgccgcgcc ggccttcacg ctggcgtaca
     1681 ggtcggagac gaagtcgcgc gtcatcgcga ggccgcgggc gacctgctcc tcgcccatca
     1741 gcgccgcgcc gcggccgggc acgagcgcgc gcggcttcaa cgcggcgatg ttggcgagcg
     1801 tggcgggcca gtcctggaag taggcgtcgc cggcgtaggg cgtggcgtcg aactcgacga
     1861 ggtcgcccga cagcagcacg cgatcctgcg gcagccagac gacggtgtcg cccttggtgt
     1921 ggccgcgccc gagctgcagg atctgcactt cgagcttgcc gagccacagc gtcatcttct
     1981 gctcgaacgt gatcgtgggc caggtgaggc ccgccggcac ggtctcgacg ttgcggaaca
     2041 ggcgcgggaa gcggccgatc tcgctcgcct tgtcggcctc gccgcgctcg acgatcaggt
     2101 cgtaggtgtc gcggctggcg atgatctcct gcggcgcata ggcgctcgca ccgagcacgc
     2161 gcaccgcgtg gtagtgcgtg aggacgacgt acttgatcgg cttgtccgtg acctcgcgga
     2221 tgcggcggat cacgtcggcc gccatcgcgg gcgtggcctg ggtgtcggcc accagcaccg
     2281 cgtcgtcgcc gacgatgatg ccggtgttgg ggtcgccctc cgcggtgtac gcgtacgcgt
     2341 gctcggagag ctggctgaag gtgaccttct tctcctcgag gtcggcctgc gacgcgaatg
     2401 tcttctcggt gctcatgcgg ggtctcctgg cgcgtcccgc tcggggcggg gatgtggatc
     2461 gtccatgggg ccagtctagc gcgtcgttcg gaacgtcgca aacgcgtttg tcatccgcga
     2521 actttgatga gacggcaccg gacgccgtgg caagatccgc cctgctcaag cccacatcca
     2581 ccatgacgag aacgaagccc gaggacgagg acaaggcgca gcgcgcggtg cagtcggtcg
     2641 aggtcggcgg ccggctgctg ctggcgctgg cggcgcacgg ggcgccgatg tcgctgaagg
     2701 atctcgccgc cggcgccggc ctgccgtcgt cgcgcgcgca tccctacctc gtgagcttcg
     2761 gcaagctggg gctggtcgaa caggatggcg ccagcggccg ctacctgctc gggccggcgg
     2821 cgctgcagct cgggctggcg gcgctgcacc agctggacgc cgtgcgcagc gcgacgccgg
     2881 tggccgaggc gctcgcgcgc gagacgggcc acgcggtggg gctggccgtg tggggcaact
     2941 tcgggccgac ggtgatccgc atgatcgacg cgagcgagcc gctgcacgtg gcgatgcgcg
     3001 cgggcaccgt gatgtccatc ctgggcaccg ccaccggccg tgcgttcgcc gcctacctcg
     3061 cgcgcgagcg cctggagcgc gcgatggccg gcccgctggg
//
LOCUS       DASVLN010000277        51349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_445017, whole genome shotgun sequence.
ACCESSION   DASVLN010000277 DASVLN010000000
VERSION     DASVLN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 51349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51349
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445017"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VF453_06305"
     CDS             complement(<1..426)
                     /locus_tag="VF453_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7687297.1"
                     /translation="MPLTTTPSLLSPPSADVPLAELAPRERILRTAHELFYREGIRAT
                     GIDRVIAESGVAKLTFYRQFESKDALVRAFLDARHARWMAWFADALSRHGGGLRAIVP
                     SMREWFASPDYRGCAFLNSVGELGPAQPQVTAIARVHKRA"
     gene            534..1001
                     /locus_tag="VF453_06310"
     CDS             534..1001
                     /locus_tag="VF453_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7687298.1"
                     /translation="MDTKPPLPPFTLETAAQKVRLAEDAWNSRDPDRVVQVYAEDTRW
                     RNRAEFPVGRAQVREFLVRKWSRELDYRLVKELWAHDGARIAVRFAYEWHDDSGHWYR
                     SHGNENWEFDARGLMTRRHASINDQPIREDERKFRWPLGRRPDDHPGLSDLGL"
     gene            complement(1012..1812)
                     /locus_tag="VF453_06315"
     CDS             complement(1012..1812)
                     /locus_tag="VF453_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7687299.1"
                     /translation="MSQIRVGIGGWTYEPWRETFYPDKLPASRELWHASRQVTAIEVN
                     GTYYSTMKPAAFERWFDETPDDFVFSLKANRFATNRRVLAEAGESVQRFIDSGLARLR
                     HKLGPVLWQFAPTKRFDPADFEAFLKLLPADVEGLRLRHVLDVRHDSFMVPEFLALAR
                     AHGMGVVFTDSPDYPSFANLTTDFVYLRLMQSRAEVVTGYPPQVLDAFADCARTWAAG
                     GQPQGLPLVGGDGAPAPAVSRDVFMFMINGAKERAPRAATEVIARLAG"
     gene            complement(1850..2422)
                     /locus_tag="VF453_06320"
     CDS             complement(1850..2422)
                     /locus_tag="VF453_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7687300.1"
                     /translation="MIDRPWAGGAGRGAQPAPMRTDRLLLRLPEDADLDVVVEIYADP
                     RTSALSPLRPLEDEDEARELLSGWRRHWRRFNHGAWAIETLAAPGRVVGFGGVTRRDF
                     EGDELPNLWYRLAPGAWGRGYATELARAALADARGRVGLGDIHAIALPENHASIHVLE
                     KLGMRRNGVLPGEHGTCTRFLLAAAARDPA"
     gene            2623..2877
                     /locus_tag="VF453_06325"
     CDS             2623..2877
                     /locus_tag="VF453_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687301.1"
                     /translation="MNRRYAYRDYQILVTAQPAGDRPGWRPEICVIAPDDRWEFVPTH
                     GSLVANDAQYCLEIGRRCGESAIQDRHIDELVARYDGFWH"
     gene            2963..3335
                     /gene="ssrA"
                     /locus_tag="VF453_06330"
     tmRNA           2963..3335
                     /gene="ssrA"
                     /locus_tag="VF453_06330"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3435..3863
                     /locus_tag="VF453_06335"
     CDS             3435..3863
                     /locus_tag="VF453_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687302.1"
                     /translation="MNSHAARSGLLYAHTVFVEAVILRRYLEPGGEPIPEAERAPPRR
                     FILSWESEGELKRASLMSFWDTPGDLVKPLFPADIRWIRGDRMLIVGEEFVRPGHMSN
                     PDARAPRTFRQAWGIRILGPRPPRNAEAPRPLPAGENASV"
     gene            complement(3874..4443)
                     /locus_tag="VF453_06340"
     CDS             complement(3874..4443)
                     /locus_tag="VF453_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687303.1"
                     /translation="MDKSDRWRKIREAIYGLIALGAVGIVAAGAFVAMDDRIPRERST
                     PVDVAEVRAPAAPPPRHHAYVYESDGIYGYRPALSEQDRQNGISTKPLLTVRYLGLKG
                     DRHTWVAFEGGPENQIEFECTSPCHFVNATPFNFMGRTGQTEVMEAVEGSMLGAVVGD
                     ILAGDLKISGFGNDRSSAQPAASPTSSAL"
     gene            4649..4822
                     /locus_tag="VF453_06345"
     CDS             4649..4822
                     /locus_tag="VF453_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687304.1"
                     /translation="MHNIVARERVVQAFTEALDANPTHHTIDIAQDVADRLGIPVESV
                     FECVDAAPEPAAA"
     gene            5082..5441
                     /locus_tag="VF453_06350"
     CDS             5082..5441
                     /locus_tag="VF453_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HEX7687305.1"
                     /translation="MTNTTPVTDGELPNRGPRVKPADVEAEIASEHYFVAGDGVYGAA
                     HKAGDHSVQAFPPSLDLLTFCVLTLRNGFTVTGESACAAPENFDAEIGKRIARQAAVA
                     KVWPLLGFRLRDAIATA"
     gene            5599..5793
                     /locus_tag="VF453_06355"
     CDS             5599..5793
                     /locus_tag="VF453_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687306.1"
                     /translation="MTTTTNAPTAWKKGDRVTVKTSRGAPATGKVLARAAEGLKGLWV
                     AVKLDDGREIKARPGSLTAA"
     gene            5850..6245
                     /locus_tag="VF453_06360"
     CDS             5850..6245
                     /locus_tag="VF453_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687307.1"
                     /translation="MTETKHLDPVIAAAYRRFLEIYQVDMTDPHVQPTASLSRGFFAH
                     GYAEAHRAAIAQQQEDAWRPMLVEPMPGRWCISRWNPSGWRESWDAGTGRWGPFSVEL
                     VSREQALEQMRRLGSAPAVVDVRDPVVPA"
     gene            6250..6630
                     /locus_tag="VF453_06365"
     CDS             6250..6630
                     /locus_tag="VF453_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687308.1"
                     /translation="MAIPDLPPPMSDGRFRFLLRQGTREEFGRVVEAVARAPLVALVA
                     TLRAELRRNALMGLASSGEAIEAAGEVERLRDALGEIRPIADAIAKGRYGIADAAATG
                     IRNIVDRALAPAESAPIPEGTSRV"
     gene            6623..6811
                     /locus_tag="VF453_06370"
     CDS             6623..6811
                     /locus_tag="VF453_06370"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX7687309.1"
                     /translation="MSEPTTSRKPRGFAALSPERRREIASMGGRTAHAAGTAHQFTSD
                     EARAAVAKRHRKPSAANV"
     gene            6804..7163
                     /locus_tag="VF453_06375"
     CDS             6804..7163
                     /locus_tag="VF453_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687310.1"
                     /translation="MSDQRALVEDAARAAGLQPAHWSDDGRFLLLVGVQYPWNPVIDN
                     GDCAALEAACSIDVNWYDERVIASIGLDIVDGIDADEFYVTHADDRQAARRLASTRAA
                     AAAWRDAASTVREGSSS"
     gene            7160..7546
                     /locus_tag="VF453_06380"
     CDS             7160..7546
                     /locus_tag="VF453_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687311.1"
                     /translation="MTKQEVPQLVPVGTVKVRHVRGPERRYTITNGALDNLATGTYHL
                     FASTGAVAADARELTVYLDEEMSALLRQTIGDDGDAHAIKLHIGDGHSGWGLYVSLAD
                     YPEEGAIQLAAIRSPDAAGEPQGEPK"
     gene            7543..8958
                     /locus_tag="VF453_06385"
     CDS             7543..8958
                     /locus_tag="VF453_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687312.1"
                     /translation="MTTPTLPPEVARLVHSKDLYELLMNVQNGIDAPGHASMANGVIV
                     MVDKARNALAHLSASAAAQGEAVAWQVWWGIGEMRPHWPPFKTENEARDHASMIRSNT
                     EVRPLYTHPAPPASASAPRAQEAQQPAAWIAWPGGEPPVEGHVVVHYRLRSGAESNNS
                     ADGLFWHHDGGEDDIVAYRRAHPAHEGGEDGELPELSDEHILALWREATSGNHGDTTH
                     QFVCWFARAIHASRAQPAAASAAVREALRIGRSWIKAVTKSSHATASERDAALVDLAF
                     IDRTVDRLAAAPDVPPAEVAQAAPLMANGMTEAETSATASVVGLSQAAPVDALPELPP
                     LPWKVPTAAAGAVFKYAEDYARAAQALVRATPAPRVESQAIDAKIGLRELPPIRMGSQ
                     EVESLEAAAKERGVILQAHVRDLLRAPRVEAKPLTEEQIREGYREVRDAGLVVNFAQG
                     VKYAERFHGVGTHPEAEGGAS"
     gene            8955..9155
                     /locus_tag="VF453_06390"
     CDS             8955..9155
                     /locus_tag="VF453_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687313.1"
                     /translation="MSQGIAAGKSLCLPFPLMPAFDVQIVVPRDMTRGEAEGLCAFVM
                     SLAAPEAAAIKHAEADSEKEAR"
     gene            9152..9415
                     /locus_tag="VF453_06395"
     CDS             9152..9415
                     /locus_tag="VF453_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687314.1"
                     /translation="MSKQSEAKERQCYTPKLIPSVCGNCAHKRSTITEHPAEFSWSKP
                     YTTEKDLRCAIGGFAVRKMGSCSEWAGTSEHPEADKEKSNGSL"
     gene            9402..9704
                     /locus_tag="VF453_06400"
     CDS             9402..9704
                     /locus_tag="VF453_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687315.1"
                     /translation="MAACDYRSCDVCGGKAFYDAELAYESMSDENSIRRGATPMRYAG
                     REQFSEPELTAKFGQVLGYVGDWAVLCTACSKTHRTAILPRESATGTQADSGVAGS"
     gene            9701..10678
                     /locus_tag="VF453_06405"
     CDS             9701..10678
                     /locus_tag="VF453_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX7687316.1"
                     /translation="MTAYYNEIDPYAAEWLRNLIAAGHIAPGEVDQRSIEDVHPDDLR
                     GFTQCHFFAGIGVWSYALRRAGWADDRPVWTGSCPCQPFSAAGQGAGFDDERHLWPAF
                     FHLIRERRPAKIIGEQVASKDAEPWLDLVQDDLEGVGYAFGAVPFPSAGVGAPHIRDR
                     LYWVADAQRTDRRRQAAAADYAGSQTERSANRAGGRSRTSVLLGDTSSVRRDEPEHAG
                     AVCREKAQAPGDCRGDGAGPAGSIIELAIAERVGPVNGFWRAADWIACRDDRWRPVEP
                     GTFPLAHGAPARVGRLRAYGNAINAEAARVFIEAFMTEPNPLPGDHHGT"
     gene            10668..11294
                     /locus_tag="VF453_06410"
     CDS             10668..11294
                     /locus_tag="VF453_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687317.1"
                     /translation="MAPDPTKPDAAALAAARQDLHRKFRELGYTTNAQTSELLDVVFD
                     RLAAMAQRPDSAGSLVGDRDLALGLLRGLHPEITRDEPMEVAQQIFDHVQAEREVASR
                     RIVRMQETIDALLRQLRGAPSQVATASAQSAKRDATSQAAQREETCEWWQDGNEDGTW
                     FSACGGDPWRFEAEDPTKNGMRFCIHCGKRMVQRDAARAAGAQGGERV"
     gene            11287..11808
                     /locus_tag="VF453_06415"
     CDS             11287..11808
                     /locus_tag="VF453_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEX7687318.1"
                     /translation="MSDRDLNITAVFSDCGRFRHILRACWDSSRPTLGWICLNPSQAG
                     RPGGDGFVVGDPSVRKMAGFSADNGYGGLVLANLFDFIATDPREMKAAGYPRSPNADD
                     WIRTAAREAGGHVVCAWGTNARKLSRPTEVLALLRSAGARTMALRLTDDGIPWHPLML
                     PYSCKLVDLEVRG"
     gene            11805..11939
                     /locus_tag="VF453_06420"
     CDS             11805..11939
                     /locus_tag="VF453_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687319.1"
                     /translation="MIALGFLLLVAVILGVLRLGASEAPPPDYGDYRGDPNGDDSAWK
                     "
     gene            11930..12079
                     /locus_tag="VF453_06425"
     CDS             11930..12079
                     /locus_tag="VF453_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687320.1"
                     /translation="MEVIESWVQLPPQRRVIPSTTHVRPYARAGGALVEKDARAIARK
                     QSTSL"
     gene            complement(12593..13105)
                     /locus_tag="VF453_06430"
     CDS             complement(12593..13105)
                     /locus_tag="VF453_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7687321.1"
                     /translation="MTTLITRSESELKVPSSASSFVARLACDAAFAAGQADGFAKGVA
                     AQTYRFIVEPENNSEIESPEFDQLLDELAADPVYASELPRARAWVAEAFYPTMTLSAL
                     RLRAGMSQRQLAEACGMEQPHISRMESGKHEPHLMQIRRISQALGVSMDEFASAWENS
                     RAALNEREDV"
     gene            complement(13556..14524)
                     /locus_tag="VF453_06435"
     CDS             complement(13556..14524)
                     /locus_tag="VF453_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX7687322.1"
                     /translation="MCYSAQVYADWRKYTRMFGATMSVGEFYRTFWQRAHEPAAKVKI
                     PKAMEHAFADPQTDDERRIKAEIDAWHASEATRLEQELFAQRKRLADAERVLAGAKPT
                     KKAADDARIAPDKIERARRALADLQRSELEPRDSRIFPGVYAPVMVVQDGQFVVMPMR
                     YQCRPCGSPAFFDVKFPGTYNARRDSLGSRFWKPLFGFSHGIMLASSFFEHVPRHKAE
                     GRDLAAGEAEEDMILEFKPAGLDEMLVACLWARWTAPGQADLLSFAAITDEPPPEVAA
                     AGHDRCIIPIKRENVEAWLRPGADVAAMQAILDDRERPYYEHRLAA"
     gene            complement(14536..14781)
                     /locus_tag="VF453_06440"
     CDS             complement(14536..14781)
                     /locus_tag="VF453_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687323.1"
                     /translation="MTNLGEKDTQDMHEIFGRLDALTAAVRALVNAFPDPDEARRILL
                     RSLDSECGRGLSPEHRTPWAQQGVDAVLDELRIPVEP"
     gene            14850..15467
                     /locus_tag="VF453_06445"
     CDS             14850..15467
                     /locus_tag="VF453_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX7687324.1"
                     /translation="MNFASRLLLLAAIAAAATPADASRRKHSLVDQAVSAYEDSHSYS
                     IPAAGAIEVAFSPNEGSEHLVVKVIDSAQRELRVLAYSFTSVPVTEALVRARHRGVDV
                     RVIADQKENVSQDRSGKARAALGALQAAGADVRTISVYPIHHDKVVIADGQTVELGSF
                     NYSAAAAHANSENVFVNWANPKLASVYLEHFARNYAQAQPFVPSY"
     gene            15524..15847
                     /locus_tag="VF453_06450"
     CDS             15524..15847
                     /locus_tag="VF453_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7687325.1"
                     /translation="MSTFGARLKQARQLAGLSQRQLGILAGIAPGSSSARMNQYERDT
                     HEAPMALVRKLARVLDVPVPFFYAEKDEHARLLLAYSKLPAAQRRAFLASVKQAAQQL
                     SSSKA"
     gene            complement(15882..16508)
                     /locus_tag="VF453_06455"
     CDS             complement(15882..16508)
                     /locus_tag="VF453_06455"
                     /inference="COORDINATES: protein motif:HMM:NF022181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 family protein"
                     /protein_id="HEX7687326.1"
                     /translation="MKTILAWIANQVPAVRWAELAILLAVVGTIAGLLGVQQYRIGSL
                     RTAVATEKAGRAADRATAATVAASATADRLAESGRRIQANQEIDREATRLETLRQRDA
                     DSLAAADVDRRMRNAFRAGVAAGGRAGGGDPGPVSVSAAASAPVDLRADVFDEWDGRA
                     RTLEAALDQAHLRGLACEAEYDALRRKASVRLALVGQDAAASAPPSSP"
     gene            complement(16520..16834)
                     /locus_tag="VF453_06460"
     CDS             complement(16520..16834)
                     /locus_tag="VF453_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687327.1"
                     /translation="MTTKFPTKGTLESVVLAYDFSLAIGTATIAAPSFTCEVESGAED
                     ASPLSVLSGDPQIDSGNAARVLQRVVGGVDGTDYRIQCTVTTSAGDTLTLAAILPVRA
                     KL"
     gene            complement(17253..17801)
                     /locus_tag="VF453_06465"
     CDS             complement(17253..17801)
                     /locus_tag="VF453_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687328.1"
                     /translation="MTVRIAPFTFDFEASQIAVDAGVTSVDVTALYRAAQQAQQSAEG
                     MLYGPLVSGSGLVPLGSGVYVGITVKLLGNWQLLFTAGAYQARVSGGNLVGGPGDDPL
                     AYSPGVQVMLVQSANATVVDVGGSITDDVAAIRALAEADEVYDHEAGLLHTYARGTTT
                     DLIPPKAVTGTTQPTDSGLVQA"
     gene            complement(17805..19223)
                     /locus_tag="VF453_06470"
     CDS             complement(17805..19223)
                     /locus_tag="VF453_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687329.1"
                     /translation="MAIQDDFSVDAGGNIRHTGGATVYSVLALHAWLQDMADNAATSS
                     DDLVSILSVNPSKLDGPRNAAIASRLNLLSTFNIDATAAQFINFGSIKQASGATLYSG
                     LKTIGSIVAGSSIYVVQNGTKLTKFWPNGHVQIMVLVTNAGSTIGDGTVTAFSRVYGQ
                     TYDYFNVDLTAGGENIAAIGTSADANITLSKAAALALSSHVTITVADSNHDIGDGAGS
                     KLYKGTIALDGSISVAQLYQYLQAITCEGQTGAINGVAGEQYQYLNAAYAPNKAAPFG
                     NFAGGKLFLAQGWWLSGVQAADSQNYQLTSHDGTTHSAPVVTTITVGNLVSGDGVLVA
                     RDNGSGGILTTEFTLNGAHAIGSGTITVNETITADHPSAGVIRVEGVRYAYTSWTGHT
                     FTLSGTTGEAHANGANAFVPLIDKTTTSTSESVSFTYSAEFTARIRVRQGSGGTPIVP
                     FETTLDVLSAGGSVNTVRTADV"
     gene            complement(19239..21809)
                     /locus_tag="VF453_06475"
     CDS             complement(19239..21809)
                     /locus_tag="VF453_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687330.1"
                     /translation="MTALAGKAGGDTYNVNGGALTIDCDTRYGPNLSPTTGPLGPITI
                     SATLGGQVKIDGTKVRLIPYTSGSGNVPAAGTSITQGGVSAELLGVWSAINTTPTTAG
                     SAMPASGFIKVRNVTGGSFAAGALSGITATARGADIVGWIEVAGKEASAFSMGKLGSF
                     TIQGAWLSPFDSTGAEIKTSGVAGQQIQLPASVTGTYYNGVWIETSAGSGVYQRYGNG
                     ADSTNAGWSTDERQRNVWISSAGVLTIGKDGGGNVGGVLPASGCRIRVPNVCLVTTNT
                     TVGLGANSTPSSTVNTRYFMPASNSGTLQFDLANFAWGMNAAPYASISVTDSTIVGPF
                     QIVNPLSAPTFQNNIVGQRDTSQADVTQCVVTTFNGGTVSDNTFSSIAIQTSFVYAGN
                     LTMNGGGYMDRNRFQVMVPRTVSGSNPGCAAINLSSMTSADSNDFVGEMIYTLTLTDT
                     KLSNSRFAARSNGTLGATVLSGQAFWNSGSGFTNATIDGWTYIVNTANACAPSNNGIV
                     VPSGSSGFKVRNFGTAAQPLSLGTVNPMSGAVIATLNNAYDLGVFVQNCWFADGNSSS
                     ASVTQLVSQATFNIEGGSIPGVAAILSASNAKFRGVRVTNAPRAAANTSGIHWADYFT
                     SDTVGAIAILMTDVVSLTSDQLSYSGTAQFSNAPGLIMPSVGDQVVWTMNYAAQGHSQ
                     LQSISMSGGTIGNYTVEFRADIGDGNGWSAWQAATNTNLQAVAITPSKGIKLAVRLTA
                     TTGTTTAITGLTIATTTTLANQQAVSYPLDPVTVTVTNCVVGSRIKITRTDTNAVLVN
                     DAVTASTYSVTGDWLNVPMQIELRCATAPGPYYQPFVAQGTPTINGLSITALQVRDDQ
                     "
     gene            complement(21847..22332)
                     /locus_tag="VF453_06480"
     CDS             complement(21847..22332)
                     /locus_tag="VF453_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HEX7687331.1"
                     /translation="MAVADNLRRRSAIVAAVCALAAPAEGIRHYAYYDPPGVLTVCDG
                     HTGNVTAGRYYSDGECYQLLQADAGQAVDTVLRCAPGAPDSVAAAFGDAVFNLGPTIA
                     CDVRKSTAARLLAARQWRGACEQLPRWDRATVAGVSVALPGLTKRRQLERGVCLQGLT
                     S"
     gene            complement(22319..22546)
                     /locus_tag="VF453_06485"
     CDS             complement(22319..22546)
                     /locus_tag="VF453_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687332.1"
                     /translation="MKLIDDAGKTWHRLWSVRLAILAALLGAADAALPYIAPAHSSPA
                     FAALTAAVSLAAAGARLVAQPKLQGDADGSR"
     gene            complement(22562..23008)
                     /locus_tag="VF453_06490"
     CDS             complement(22562..23008)
                     /locus_tag="VF453_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687333.1"
                     /translation="MTDPLQDRDEDETPSRGEMSSVGAIHELSRVEIKGLSEDEWREL
                     VLQRLEHGRVRMDGMEASLAANTAETAKTAASVAQALPMIGHIHEIVETAQSFFRGLA
                     KVSAFFARMWLWLGPVVRAVSVLAGAATAVKVAYITFRGGNPPRSH"
     gene            complement(23031..24839)
                     /locus_tag="VF453_06495"
     CDS             complement(23031..24839)
                     /locus_tag="VF453_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX7687334.1"
                     /translation="MDPLWKSDASSQAWEPVTTRSIGYPRAGDLATGQHPTHIGGEWF
                     EDMLEEIKSTIESAGMTFDPTDVTQFLSAIYALILRPDFVAAPTSGTAPLTVSFTDTS
                     TRGRADTHLWDFGDGHTSTDASPTHTYGGQGTYSVTMTVTLNGAVRSVTKAAIISVAA
                     APDDSTVFLLHFEGTAGSQTFTDSGIRGLTFTPADTGVTLVADPRAGATSGRFSSSNG
                     ISTSWPAGFSMAGPFTIEFSWKPTDLGAKHLLFSSAAGGVNGDRMMIVLFDGGELQWY
                     QGVYGATDTRAPFPDLEVVANAWNDVAITRDAHGVVRAFLNGQVSATTVIDNNDYTHA
                     GAVITVGGLNAPGTNATTFELDEVRLASACLYTDNYPVSLPLAGAPGHEVDPYFSAVG
                     LLMNGDTLTDAAGHTVLTYAGTGSGTPLVNTTDPDFPHGAIDVSPDRGYLGITAFGTE
                     LDFGAGDFTIELDYKARAKSSAYPTMLTTYLDGSTYGANIVDHHDLSPGNLAIAGGPE
                     FPIFPFATSIGTLYRMCLQRAGGTLMLFVNGAMVASRADSHHWTVQGAVDIGNDTANS
                     GQCAFDGLIGRIRITKGIARHSPAGYVTNSDLWPTS"
     gene            complement(24843..25970)
                     /locus_tag="VF453_06500"
     CDS             complement(24843..25970)
                     /locus_tag="VF453_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0031221 - arabinan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosidase catalytic domain-containing
                     protein"
                     /protein_id="HEX7687335.1"
                     /translation="MDRVYQANVSATPPTPPSPGAHGHVQSDVPHPLQATVPPTTPGP
                     WIFHYVTESFMRVIEAAGLTPDATNLHQLADAVVALASAPARAPLDALSVQPAMAFST
                     RLLLIDYGGACMRVRRSSDNSEMDIGFARGTVDTAALLAFVGSGNGYITNWYNQTGSG
                     IDAVQANASAQPLIVESGAVLEIGGFPAALFSTANGLVFNNAAGARLDFSLGAVFATT
                     QSNGQTSTGLGYQVSGFLYADTSGSSRDFGFGNLGNTLTLWEGGAGDDLGPETGVRGA
                     TVINDGALHVGICRRTSTSGAVDVYLDGSLQASGTAARGERSDAAFCRIGSGGAGDGY
                     AVNTSPFPAAFSTPEILVYASRLSDADVELLQAAQAAYHGG"
     gene            complement(25973..26212)
                     /locus_tag="VF453_06505"
     CDS             complement(25973..26212)
                     /locus_tag="VF453_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687336.1"
                     /translation="MEPRAYRSGAAGTPPALPASAVTGYPKDADEASGATVPGPHWFY
                     MVGEEVRNLQIRGGQAPAIGAQTQMLAAIKSIAGS"
     gene            complement(26212..26679)
                     /locus_tag="VF453_06510"
     CDS             complement(26212..26679)
                     /locus_tag="VF453_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687337.1"
                     /translation="MTRYYSKSTSGFYVDAIHGEAMPTDAVEISDDDYGALMTAQASG
                     KSIVAGADGKPVATDPPAPTIDQQRATLSRAVQAHLDAVAKSNGYDSMATAVTYADEP
                     AVPTYQADGKALRAWRSEVWSAALALINAQGALPTAAAILAALPAYVAPTAAS"
     gene            complement(26681..27472)
                     /locus_tag="VF453_06515"
     CDS             complement(26681..27472)
                     /locus_tag="VF453_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687338.1"
                     /translation="MQASQVPAFFSIPWANGAGAGYVRDIPTPSQIGSEPGAASLTDG
                     FPPLNFLDPTTAGGVPPKGQDFNGILRWITQWIRWQQAGGFPGYDAGVSTAIGGYPAG
                     AVLVSADGTHFWKSTVDNNTSDPDTGGANWTVVQQGSIPFADITGVPNFLLTSDFAST
                     LAAAFTSGQSLGTSGYQKLPGGLIVQWGTYLAATTDTAYTVSLPIAFPTAHLRSFATV
                     DTSGTVAGAWSAYATPGSLSTVLLTADVQSDSASNVPLAFLCIGK"
     gene            complement(27486..28220)
                     /locus_tag="VF453_06520"
     CDS             complement(27486..28220)
                     /locus_tag="VF453_06520"
                     /inference="COORDINATES: protein motif:HMM:NF022494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HEX7687339.1"
                     /translation="MLPAGYLDPSVESTVEMPVDAELYPRVPPAWPPTVLQQYANSPR
                     LLGMISSFAVALDADAMTEMFLDSVWDIDTAVGYGLDVWGRIVDVRRGLYVPGGQSGK
                     TFGFGEAGGPLVFGFGQQPFASVQTGTPNFLLADDDYRRLILVKAFSNISDRSIHTMN
                     RALMLMFAGRGGNVSVVDYGGMKAAINFPFQPTALDLAILQQSGAFPAPSGVQLFVVV
                     LNPHHVLGFGEAGENVGSFATGAFNH"
     gene            complement(28223..29635)
                     /locus_tag="VF453_06525"
     CDS             complement(28223..29635)
                     /locus_tag="VF453_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX7687340.1"
                     /translation="MSSTQVPALTIGDNGWIAPDDADILAGRQADINAAFGTTLNPAL
                     NTPQGQLATSEAAIIAGAYATFMAVMAGVDPATSSGRAQDAIARIYYLTRNPAESTVV
                     QAVCSGKTGTVIPVNAQAVDQDGNLYLCTEAGTIPSGGSITLPFACAVTGPIACPAGF
                     LERIYQAIPGWDSVANVAAGVVGSDAESAADFEYRRQQSVALNANSTTAAVRAAVLAV
                     PGVIDAYALENPSDATSGATFTGSIAGNVLTVTGAAGVISVGDVVLGSGVVEGTLISS
                     YLAGSGGDGTYALNIAQTVAGESMACAPGGVQLKPHSIFVAAYGGDAQAIGAAILTKK
                     NPGCDMNGNTTVAVQDTSSLFAAPYPTYRISFTVPAPTGIKVAVAMQNNGKVPSTAAA
                     QIQAAVVAAFNGTDGGERARIAGEVFAARFYAGIVSLGAWAQLKSVKVGITAADQDSI
                     QLRADQIPTLSTSDIAVTFT"
     gene            complement(29646..29999)
                     /locus_tag="VF453_06530"
     CDS             complement(29646..29999)
                     /locus_tag="VF453_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687341.1"
                     /translation="MAATVYLNPTTWDLDISADGNIALAAEPYALAQDAASAIKTFQG
                     ECFYDTTIGVPLRQEILGQSPSTALVKSLLQAAALTVPEVASAEVFITGIAGRVVQGQ
                     VQVTSTSGATSSATI"
     gene            complement(29999..30613)
                     /locus_tag="VF453_06535"
     CDS             complement(29999..30613)
                     /locus_tag="VF453_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX7687342.1"
                     /translation="MRVEAVSNDGGVSPVGTVDILPLVAQVDGFGVAQAHGTIYGCPY
                     MRLQGGANAVILDPQVGDIGIAVFASRDISSVIVNKGPANPGSRRTHSMADGLYIGGV
                     LNGTPEQYVQFSNAGITLSSPNAVTLQAPAVTIDASAVVVAADTVTVNATEAITLAAE
                     TTSIIGDLNVTGTMSAGDIKQGGKSIGAPHTHNLNGGGHTLGVT"
     gene            complement(30723..31598)
                     /locus_tag="VF453_06540"
     CDS             complement(30723..31598)
                     /locus_tag="VF453_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687343.1"
                     /translation="MALVRRKITLQFAYGTGPSGDGPPQSVTLSGHRVSVNVANAGGA
                     GMPTAHVRIFGLTLSMMNQLSTMGQLPNAYRANTISVYAGDDVIGMAKIFEGTIQEAW
                     ADMQAAPEVAFNITAFAGLIDALRRSPPLSYPGTADAAVVLSNIADRMDDVAFENNGV
                     SVILSTPYYPGTLREQALAVVQHAGIEWNGIENGVLAIWPRGGSRGGLVPRIAKDTGM
                     VGYPTYTSTGLIVTTTFNPVVRHGGQIDVESSLTPACGRWVVAMLTHDIEAEVPSGKW
                     LTLMQVAKPGYAPVV"
     gene            complement(31598..31921)
                     /locus_tag="VF453_06545"
     CDS             complement(31598..31921)
                     /locus_tag="VF453_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687344.1"
                     /translation="MMNIPLSALPSQTVSALLGGQSCRLKVYQRTTGMFIDVYVDDAL
                     ILGGAMLRDRVLVLRDAYFDFVGDLMMIDTAAESDPFYSASEPTIGSRFILLYVEAAD
                     FAALA"
     gene            complement(31918..32550)
                     /locus_tag="VF453_06550"
     CDS             complement(31918..32550)
                     /locus_tag="VF453_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687345.1"
                     /translation="MADGAPTLLSGMSAPAAARDTTDTARSTGAKSAEWGIFFNGSKA
                     LEPDSIVAMDYQREWSLLDYQVEQGAFETFNKVARPFDTRLRITKGGSQSDRTAFLAQ
                     VEALAESLNTYDVVTPERTYLSVNVSRLGYGRSARDGATLLIVDIGLRQIRTTAVAAF
                     TQVASPSAASPVVGGTVQPVATPAPQKAIADAALKRQASLPLLALKGQLR"
     gene            complement(32550..34259)
                     /locus_tag="VF453_06555"
     CDS             complement(32550..34259)
                     /locus_tag="VF453_06555"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7687346.1"
                     /translation="MPTIIDSLLVTLGLDPRGYKKGAKEAEDAHNSLGRGSSKVNKEL
                     QAQLKKSQDAVATLRNEVLRMAAVFVGMSAIKSFIESSTKFDAATGRMAKTLDMSTEG
                     LSTWQQVAEQMGVSGQAVAGDMDKLTQSLERFSATGEGGDNFRFFRALNVQISKLGSQ
                     EARDARDVLLDVADAFKGMDPKRAKMMGLGMGFSPETISMLLKGRKELEGALDRARST
                     ALSEDDVRAAQDRQKAWAELEQTFGRLARDVENELTPSMVGLANFVKQHAPETAFAIS
                     AITASMTALSAVRFIGLISSLRGLTAAAKAASATAAVAEGAGAVGGLGGLAGARAGLA
                     GMGLLGQANIYAGAAVGATEIARLGVLLKEWWDVEHRQGAKLTPDAAARLAQLNAAGG
                     SAVPTTGDSGSKQEYLRSLEQKWGLPAGLLDSVWQEESGRGQHMVSPKGAMGDFQQMP
                     ATARAYGITDPMNFQQSASAAARMLADLLRQYGGNLPTTLAAYNFGSGNLQRSGGALP
                     LETQKYVRDIMGRLRGSGGSGTNNETTIENMTIVTQATDARGIARDMQPALIAAQANT
                     GMF"
     gene            complement(34261..34413)
                     /locus_tag="VF453_06560"
     CDS             complement(34261..34413)
                     /locus_tag="VF453_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7687347.1"
                     /translation="MPRAIGVVVSSRLASMSELSTTLGTHDLYDLLEILAVDAHNERV
                     LSTPKG"
     gene            complement(34452..34868)
                     /locus_tag="VF453_06565"
     CDS             complement(34452..34868)
                     /locus_tag="VF453_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687348.1"
                     /translation="MARREAFVTIDAEGRDKGKVFKLTEMPAAQAERWAIRAFLALAN
                     AGVDLPEDVATSGMAGIAVVGIRALGKLKWADAEPLLDEMMLCVSACPQPDNRTVVTG
                     LFPDSIEEISTRLRLRKEVFLLHTGFSLPAAPSTSA"
     gene            complement(34868..35305)
                     /locus_tag="VF453_06570"
     CDS             complement(34868..35305)
                     /locus_tag="VF453_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687349.1"
                     /translation="MANKTLTAANSIITISIADLVPSPVQLQGFAADAIYDTEAIEQA
                     QTSMGVDGKLSAGLVLMETPWNVHLQADSDSNQLFDTWIAQQKQQREVYFANGSIVLP
                     SLKTAITLTKGVLTRAPMMPNAAKTMQPRAYTIVWESVTPAGI"
     gene            complement(35342..36844)
                     /locus_tag="VF453_06575"
     CDS             complement(35342..36844)
                     /locus_tag="VF453_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HEX7687350.1"
                     /translation="MTASIPAAYFVEVTPSVIKAGGNALVLNGVVLTESTRAPLGAVL
                     SFASADGVAAYFGDSSVEAQVAAIYFNGFDNSDVKPGSILFTQYNASAVAGWMRGGTS
                     QTPILSELQALSGVLTLTVDGTPHTSSNIDLSAATSFSNAATIIQAAFTSPGFTVSFD
                     SVSAAFVFTSNTTGASSSVSVATGSLAAGLALTTATGAVTSPGAAAAVPGTFMDGIKR
                     ATQNWATFMTAFDPDGGSGNTVKQAFAAWVNSQNKRFIYVAWDDDVTPTESTSAAASL
                     GAILVAANSEATCPIYGPTWDKAAFICGSIASIDFTRTNGRPNFAYRSQDGLPADVTS
                     DTVAANLQANGYNFYGAVATANQGFEYFYPGSVTGTWSWLDSLVCEIWLNNALQLALL
                     TLLTIVKSIPYNQAGYALIRAACMDPINAALNFGAIRAGVTLTAQQIAEVNNQAGTQI
                     DQVLSTRGWYLQVNDASGTTRAARSSPPITLWYMDGDSVNKLSLSSIAVQ"
     gene            complement(36933..37490)
                     /locus_tag="VF453_06580"
     CDS             complement(36933..37490)
                     /locus_tag="VF453_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687351.1"
                     /translation="MSATVDITEELTLAALRSFLLAILPAGVDVIVGQSNRVPEPMAG
                     DFVVQTAFFKHRLSTNRTTYADGRLTDPVAGAGQRQDTVPQQVTVQLDFHGPSSYDNA
                     TIVEALWRSDAGVDGLAISGLAIAPLYASDPRQAPFLNGEQQVEQCWQVDLVMQCNPV
                     ITTGQDFAASLEVGLINVDREFPPA"
     gene            complement(37487..37870)
                     /locus_tag="VF453_06585"
     CDS             complement(37487..37870)
                     /locus_tag="VF453_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687352.1"
                     /translation="MNLSALVGGVIGAVNPKVQAQYSASTGSASAPSGRRTPTYADSV
                     SVLVQKQALTYKDLQQLQGVNLNGEACAIYVEGDWRGVSRPAGRGGDMLTLDDGTVWL
                     VVHVLENFFQTAGWAKVAAVLQNGQ"
     gene            complement(37875..38420)
                     /locus_tag="VF453_06590"
     CDS             complement(37875..38420)
                     /locus_tag="VF453_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687353.1"
                     /translation="MKGLNFDKIRARLEAIPEGFAERVAKVGWFPAAQYKDGTPVAYV
                     AAIQENGYSAGGIQARPFIRPTIADRKGAWVKVMADGMRAVVKGRASAEQVLDGVGLQ
                     AAGDIAHTLGTASFAPLSPVTLMLRKMRDDDPSLVMNRRAVAEAAARVARGEAGSTRT
                     DPLHDTGLMISSLTHLVGEAE"
     gene            complement(38417..38845)
                     /locus_tag="VF453_06595"
     CDS             complement(38417..38845)
                     /locus_tag="VF453_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HEX7687354.1"
                     /translation="MAAHTFDQDAFCKSFPALAQVPADQLAVYFQDATAFLGENDTCI
                     LRGAVMDRALSLLTAHLAQSAAMLQAGQTSVAPINGATQGSVSVQMQPPPTTSGWQFW
                     LASTPYGLQLWALLSMRAAGGFLIGGSPERSSYRKAGGVW"
     gene            complement(38855..39235)
                     /locus_tag="VF453_06600"
     CDS             complement(38855..39235)
                     /locus_tag="VF453_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687355.1"
                     /translation="MAKVYIYSTLTSDMHYTAWLPPVEKGAIANVERVVFIRGGSGVA
                     NDRIVTPSGVRTEIDDSDLEVLQKNEVFKLHVKNGHMRVERKDVDAEKVAADMNRADP
                     SAPKTPADYANAGDDDAKPAAKNM"
     gene            complement(39326..40459)
                     /locus_tag="VF453_06605"
     CDS             complement(39326..40459)
                     /locus_tag="VF453_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid family protein"
                     /protein_id="HEX7687356.1"
                     /translation="MKSQEHGYLSPRSFQLPDGMPRRLEMKASDVANYLALSAVGIGL
                     PRGKIDRMAEIALDEFAMDDAQGLLTAASITTPVQFLQTWLPGFVRTITAARKIDSLI
                     GMTTIGDWEDEEVVQGVLEPVGTAVPYGDLTNVPLSSWNTNFERRTVVRFEKGMQVGV
                     LEERRAARIRLNTAAEKRNSSATALEIQRNLVGFFGYNSGNNRTYGFLNDPSLPAYYT
                     VPNGTGGSPTWASKTFLEITADIRQAATKLQTQSQDTVNPLTDATTLALPTAVAQAVT
                     TATDFGMSVRKWVAENYPKMRIESAPQLNGANGGANVFYLYAESVDDGASDNSRVIEQ
                     IVPAKFMVLGVEKRSKAYVEDYSNATAGVMVKRPYAIVRGSGI"
     gene            complement(40476..40991)
                     /locus_tag="VF453_06610"
     CDS             complement(40476..40991)
                     /locus_tag="VF453_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687357.1"
                     /translation="MTAFQSTVNITYAFGVPGEFNKDGPRRVVRRPINSGGANPNVIG
                     YAYTAANGTGVASVGGAITNGTSIFAGILANPKEYSSSGGSSGPLSPTLTLPDNSEGD
                     FVLMGNGLVVALTTAANMGDQVIYNTTTGALAAVAPGAAAGAGNAYVPNCFVEEVVNA
                     AAGLVSIRLTN"
     gene            complement(40995..42557)
                     /locus_tag="VF453_06615"
     CDS             complement(40995..42557)
                     /locus_tag="VF453_06615"
                     /inference="COORDINATES: protein motif:HMM:NF021494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HEX7687358.1"
                     /translation="MTEESKITPTAAGIAYVADGRVLLLKRSASTDAHPLTWGFPAGG
                     IEEGEGSLEAAARESMEETGHRPDVVAPLEERDGFALFLCTEPAFAPVLNDEHLGYCW
                     ASPDDLPTPLHPMVADQIALALAAAAPAPEEPAEPDDVAMDESARDFDTNGWFEIKGN
                     PLSKVGVFPYRGRQVGKPDEPDRLFQILRPAEELSDPEAIASFRLLPWIDNHVMLGPE
                     EAGLMPAERKGVQGVVGEDVYFDPDTDDGVLRGNVKLFSQSMATLIEAGKRELSCGYR
                     CKYDWTPGVFKGQPYDAIQRRIRGNHLALVKSGRMGPDVAVLDSIDQPTTSEKEPSMA
                     DEKKDEGGSGMTLEAALDAFKQVMPAIKMLMEHANPGAATTTAAAVDADPGKTEEEKK
                     DEAKTVAAKDDDSGTPPPKDDDDKGSAAMDEAAVFRSVMGQVVRRDKLARELSAHIGT
                     FDHSEMTEAEVAAYGVKKLGIKAAAGQEAAVLSGYLAAAVAPSAKKATTAVAMDSSSG
                     GGDFVTRFLQGE"
     gene            complement(42619..43467)
                     /locus_tag="VF453_06620"
     CDS             complement(42619..43467)
                     /locus_tag="VF453_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HEX7687359.1"
                     /translation="MATIPRPNEPLRGGVLSPNAAIQRDFEVAILAMIRRLADETKKT
                     VARVFADAAHDAADAAPREGAIAGFSNISSQARIQLNALMDKYEPMFNRLAKKATKRM
                     MDRTIQNSAVTLGMSLREISQSVQLNAKAMSPALLDIINASTTEAVGLIKVIPTTYLT
                     KVQGAVMRSITTGQGLKDLTPFLEKAYRQNVRHARLVAMDQTRKAYSGITAQRMLDID
                     CPEYEWVHTAGSKEPRIDHIEMSGSVYSLKDPPVIDKRTGQRGKPGDAIGCRCKMRPI
                     VRFHAD"
     gene            complement(43442..44776)
                     /locus_tag="VF453_06625"
     CDS             complement(43442..44776)
                     /locus_tag="VF453_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HEX7687360.1"
                     /translation="MDDDGNGDGIALKLAATAQNIPEVLAAWYASQTFIGHQLAAILA
                     QHWLIDKACGMPGRDAIRQGFDIVSASGDEIPPEAIKILKAADRRVKLNAQLEEFVRK
                     GRIFGIRIALFKVESTDTEYYEKPFNPDGVTPDSYKGIVQVDPYWTAPMLDQAASSRP
                     DTAHFYEPTWWQINGQRYHRSHLVIFRNSEVPDLLKPQYLYGGVPVPQRIMERVYGAE
                     RTANEAPQLAQTKRTTVWRTDMSQFAALGDEGVERMSTWARFRDNYAIKLGDKEGDEF
                     TQFDTSLADLDSVIMTQYQIVAAAANVPATKLLGTSPKGFNATGEFEEASYHEELESI
                     QAHDLTPLVERHHLLVMRSEVVPKLAAMKDIETTVEWRPVDSPTAGEQAELNLKKAQT
                     GQALVSSGAIGSEEERQRIALDPASGYAGVDLTAPAPDDDLEDDDGDDPAAE"
     gene            complement(44951..46348)
                     /gene="terL"
                     /locus_tag="VF453_06630"
     CDS             complement(44951..46348)
                     /gene="terL"
                     /locus_tag="VF453_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEX7687361.1"
                     /translation="MRVFSPQERFAAAELARADHYFFSRWMFQQRKGFQWQRARHHKL
                     ICDALMRVYRGECKRLVINIPPRYSKTELAVINWIAWCFGQVPDAEFIHTSYSSQLAV
                     NNSAGIRSMVQHEAYREIFPGVALSGEAAHHWKTTAGGVMYATGTGGTITGFGAGKHR
                     EGFGGAIVIDDPHKADEARSDVIRQGVIDWFQTTLESRKNSPDTPIIVIMQRLHQDDL
                     AGWLLGEQPGIAPGGNGEVWEHLCLSAWNDDGTPLWPEKHDAEALRRMESASPYVFAG
                     QYRQRPAPLDGGLFKPDQISVIDALPAGRIDWVRGWDLASTTDGDWTVGLRLGRTEDG
                     RFIIADVNRMRCGPDQRDAAIVNTAARDGRPTKQSIPQDPGQAGKTQVLYLTRQLAGY
                     KVSTSPETGDKVTRAEPAAAQVNVGNVSMLRGDWNAAFISELRVFPNGSFDDQVDALS
                     RAFGELIVVRRSFFG"
     gene            complement(46352..46834)
                     /locus_tag="VF453_06635"
     CDS             complement(46352..46834)
                     /locus_tag="VF453_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2280 domain-containing protein"
                     /protein_id="HEX7687362.1"
                     /translation="MAKLTEPQKLFIVQALACFDTPSQIVDAVREEFGIEIHRRQVAE
                     YDPSKVSGRGQISAKLVALFESTRAAFLKETSQIPIANQAVRLRTLQRLATLAENQRN
                     VALAAQLVEQAAKEVGGAFTNRREITGKGGGPIEQASTTTSVTLEEFQQIARRVADEV
                     "
     gene            complement(46857..47483)
                     /locus_tag="VF453_06640"
     CDS             complement(46857..47483)
                     /locus_tag="VF453_06640"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HEX7687363.1"
                     /translation="MARTSAPRVPRRPRPPADGRMQPAAEVLEWIERYILRETGELHN
                     PEHRHLIGADLQVMWAPQGFAKKGRTVVGTAEEVSFRSGGFERMRAEQQYADWFGVAP
                     KFLITFAASYAADCTDAEWCALVEHELYHVAHARGLFGEPLFTKEGRPRLEIRGHDVE
                     EFVGVVRRYGTGNPEGNLARLVEAAGRAPEVSRLQVAGACGTCLLRAA"
     gene            complement(47486..47764)
                     /locus_tag="VF453_06645"
     CDS             complement(47486..47764)
                     /locus_tag="VF453_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687364.1"
                     /translation="MTPRAPVEGVLLTHKGWFGVCPVYLGDLDTEAPLIDPRSPLLIP
                     LFLLSEAMFGLCFTVLAAAGRENPGWALKITGKLRRPIRIEAPRICED"
     gene            complement(47769..48008)
                     /locus_tag="VF453_06650"
     CDS             complement(47769..48008)
                     /locus_tag="VF453_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687365.1"
                     /translation="MPTIREAMQAEVDRLRTQADAIEAKLKSVEPSLANVLEHDAAAV
                     ASFFRAFGEHIFGHKPAAAPAPDAQPTAPATTAAQ"
     gene            complement(48605..48946)
                     /locus_tag="VF453_06655"
     CDS             complement(48605..48946)
                     /locus_tag="VF453_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687366.1"
                     /translation="MHYKDGREAKVGDLVKGKVYNTPGVVVGVMLGITPGAPTCNCRV
                     GIVRYRDAGDIGKVEQPYAHAVCLSDGHFQVHRERIGHGPITATLVGVKVDYGQCDQL
                     ELVHRDGESPD"
     gene            complement(48966..49041)
                     /locus_tag="VF453_06660"
     tRNA            complement(48966..49041)
                     /locus_tag="VF453_06660"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49006..49008),aa:Met,seq:cat)
     gene            complement(49060..49347)
                     /locus_tag="VF453_06665"
     CDS             complement(49060..49347)
                     /locus_tag="VF453_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687367.1"
                     /translation="MIRSLRVLPSLALAVVMSVASTAFDGLARVVAAARDAFAFAVSV
                     VRSAFRVEPAAGGRVDMRSSAALTAASAFVKRMVKRDAPRIEARWSMCPSI"
     gene            complement(49413..49685)
                     /locus_tag="VF453_06670"
     CDS             complement(49413..49685)
                     /locus_tag="VF453_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687368.1"
                     /translation="MSYSFTIRAANKAEAKNSVAAKLAEVVRHQSTHAVDQDKAYAAA
                     CAFIDALVDDDTRDIHVGMYGNVTTSGAGVIGAGVNVSASLADRTA"
     gene            complement(49954..50724)
                     /locus_tag="VF453_06675"
     CDS             complement(49954..50724)
                     /locus_tag="VF453_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687369.1"
                     /translation="MRTTNDEPVTMAERYSGAIESSNLRVRETAGDVDLLVAAGLAGN
                     CLAGALLRLQVEYDTVRGEHRAAEARLRAAEIDAARQKGDFVDEDGVVLSTAEQRASA
                     INSAAQAQALTDHVMIIATLPSLRRTKEMLGHFALARARELRFVLPTERILRLAGAAL
                     DVFLSPQCRECRGIGFTGALQRGEPQKRCRACKATGSRRYSVGKDEIGRAFVFALLLD
                     LSDLVDTAAASLARQRRAVREAKAMLLRAEAAGSGAVG"
     gene            complement(50721..51014)
                     /locus_tag="VF453_06680"
     CDS             complement(50721..51014)
                     /locus_tag="VF453_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687370.1"
                     /translation="MKPELVPTPEQALQARVAAAHARGDRITAISDSDSYSEVVLVGI
                     AIGDAAAFVMTIDRAEYDGFKVLKAMGVPCDGPAPTANAIDRAKRAQKKGTKA"
     gene            complement(51011..>51349)
                     /locus_tag="VF453_06685"
     CDS             complement(51011..>51349)
                     /locus_tag="VF453_06685"
                     /inference="COORDINATES: protein motif:HMM:NF018769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease domain-containing protein"
                     /protein_id="HEX7687371.1"
                     /translation="RRPVAGAVEAWGARIREAARGEPCTVRLPGVCRFAPEFSIWSHA
                     RWGARLGDAGRGMATKALDVCGAIACTACDGVYDGQIAAPHLTRDEIDLAWCMGHFRS
                     LGRLLEKGVL"
BASE COUNT         7867 a        17332 c        17639 g         8511 t
ORIGIN      
        1 cgcgcgcttg tggacacgcg cgatcgccgt cacttgcggc tgcgccggtc cgagctcgcc
       61 gacgctgttg aggaacgcgc agccgcggta gtcgggcgag gcgaaccatt cgcgcatcga
      121 cggcacgatc gcgcgcaggc cgccgccgtg gcgcgagagc gcgtccgcga accaggccat
      181 ccagcgcgcg tggcgcgcgt cgaggaaggc gcgcacgagc gcgtccttgc tctcgaactg
      241 gcggtagaac gtgagcttgg cgacccccga ttccgcgatg acgcgatcga tgcccgtcgc
      301 gcggatgccc tcgcggtaga acagctcgtg cgccgtgcgc aggatgcgct cgcgcggcgc
      361 gagctcggcc agcggcacgt ccgcggacgg cggtgacagc agcgagggag tcgtcgtcag
      421 gggcatgcgt ccgattgtag acaagctcgt ctacctgcgg ctagactgcg gcggacgcca
      481 ggtagacaag tacgtctaca ttcaagttca cgctccactc cccacaggcc gccatggaca
      541 ccaagcctcc gcttccgccc ttcaccctcg agaccgccgc gcagaaggtc cgcctggccg
      601 aggacgcctg gaactcgcgc gaccccgacc gcgtcgttca ggtctacgcg gaggacacgc
      661 gctggcgcaa ccgcgccgag ttcccggtcg gccgcgcgca ggtgcgcgaa ttcctggtgc
      721 gcaagtggtc gagggagctc gactaccggc tggtcaagga gctgtgggcg cacgacggcg
      781 cgcgcatcgc ggtgcgcttc gcctacgagt ggcacgacga ctcgggccac tggtaccgct
      841 cgcacggcaa cgagaactgg gagttcgacg cccgcggcct gatgacgcgc cgccatgcga
      901 gcatcaacga ccagccgatc cgcgaggacg agcggaaatt ccgctggccg ctcggccgtc
      961 ggccggacga ccatccgggg ctgagcgacc tgggcctgta ggctcagggc ttcagccggc
     1021 caggcgcgcg atcacctcgg tcgcggccct gggtgcgcgc tccttggcgc cgttgatcat
     1081 gaacatgaac acgtcgcgcg agacggccgg cgccggcgcg ccgtcgcccc cgaccagcgg
     1141 caggccctgc ggctgcccgc ccgcggccca ggtgcgggcg cagtcggcga aggcgtccag
     1201 cacctgcggc gggtagcccg tgacgacctc cgcgcgcgac tgcatgagcc gcaggtagac
     1261 gaagtccgtc gtcaggttgg cgaacgaggg gtagtcgggc gagtcggtga agacgacgcc
     1321 catgccgtgg gcgcgggcga gcgcgaggaa ctcgggcacc atgaagctgt cgtggcgcac
     1381 gtccagcacg tggcgaagac gcaggccctc gacgtcggcg ggcagcagct tgaggaaggc
     1441 ctcgaagtcg gcgggatcga agcgcttggt cggcgcgaac tgccacagca ccggcccgag
     1501 cttgtggcgc aggcgcgcga gcccgctgtc gatgaagcgc tgcaccgatt cgcccgcctc
     1561 ggccagaacg cggcggttgg tcgcgaagcg gttggccttc agcgagaaga cgaagtcgtc
     1621 gggcgtctcg tcgaaccagc gctcgaaggc ggccggcttc atcgtgctgt agtaggtgcc
     1681 gttgacctcg atggccgtca cctgccggct cgcgtgccag agctcgcgcg acgcgggcag
     1741 cttgtccggg tagaaggtct cgcgccaggg ttcgtacgtc cagccgccga tgccgactcg
     1801 gatttggctc atcgggtctc cttcgctcgc aggtcgggga agcgcgatgc tacgccggat
     1861 cccgcgccgc cgcggccagc aggaagcgcg tgcaggtgcc gtgctcgccc ggcagcacgc
     1921 cgttgcggcg catgcccagc ttctccagca cgtggatcga cgcgtggttc tcgggcagcg
     1981 cgatcgcgtg gatgtcgccc aacccgacgc gtccgcgcgc gtcggcgagc gccgcgcggg
     2041 cgagctcggt ggcgtatccc cggccccagg cgccgggcgc gaggcggtac cagaggttgg
     2101 ggagctcgtc gccctcgaag tcgcgccgcg tcacgccgcc gaagcccacg acgcggccgg
     2161 gcgccgccag cgtctcgatc gcccaggcgc cgtggttgaa gcggcgccag tgccgccgcc
     2221 agcccgacag cagctcgcgt gcctcgtcct cgtcctcgag cggccgcagc gggctcaggg
     2281 cgctcgtgcg cgggtcggcg tagatctcga cgacgacgtc gaggtccgca tcctcgggca
     2341 ggcgcagcag gaggcggtcg gtgcgcatcg gcgcaggctg ggcgccgcgg cccgccccgc
     2401 cggcccaggg gcgatcgatc atggtccggc gtccttgacg gtcgagtccg gcatgggccg
     2461 attgtcgccg accaggcacg ctccgggcgc gcggctcgac cgtgtcccga gtccgcgccg
     2521 aaggcccgaa ctggcggcga gatcgggcgc atctcccgtg atcgccgccg cgcgcgcggc
     2581 ccgagactgc gtcgtcgtcc tccttcacgg gtacctcgcc gcatgaatcg ccgctacgcc
     2641 taccgcgact accagatcct cgtcacggcc cagccggcgg gcgaccggcc cggttggcgt
     2701 cccgagatct gcgtcatcgc gccggacgac cgctgggaat tcgtgcccac gcacggctcg
     2761 ctggtcgcca acgacgcgca gtattgcctg gagatcggcc gccgctgcgg cgagagcgcg
     2821 atccaggatc gccacatcga cgagctcgtc gcccgctacg acggcttctg gcactgaacg
     2881 ggacgccccg ggcgcaaaaa atgcgccggc ggaacgccgg ggcctcttga aggcacgcgc
     2941 catgccgtaa gataggtctc ctggggccga cctggtttcg acgagggtac ggatccggcg
     3001 cagggcatgc cgagcaccag ttcgctcgta aatccactgg aaacaaacta actgcgaacg
     3061 attcttcgta cgctctcgcg gcttaacacc cgtgagcctc tcaacggttg gccgatgggc
     3121 cgggttcgag atcgcaagat cctgagctga gaggtcattc acattggctg gctatccgtc
     3181 gggccgctcg acgggtagcg agacctagcg gctggcgtca ccggtagcgt gctcctgcgc
     3241 gaccggcggc gcgagactca aaccagagag ctacgcatgt agaactgtcc ggtgatggct
     3301 ttcggacggg ggttcaattc cccccggctc caccaccaac gccagcggcc gccaatgaga
     3361 acacctcgtg gcggccgctt cctttttggc gtttaccccc ttttgctggc gttcgattcg
     3421 cacgtgattc gcacgtgaat tcgcacgcgg ccagaagtgg tctgctgtat gctcatacag
     3481 tgttcgtcga agccgtcatc ctccgccgct acctcgagcc cggcggggaa cccatccccg
     3541 aggccgagcg cgcgccgccg cgccggttca tcctgagctg ggagtccgag ggggagctca
     3601 agcgcgcgag cttgatgtcg ttctgggaca cgccgggtga tctggtgaag ccgctgtttc
     3661 cggccgacat ccggtggatc cgcggcgacc gcatgctcat cgtgggcgag gagttcgtgc
     3721 ggcccggcca catgagcaac cccgacgctc gcgcgccgcg gacgttccgc caggcgtggg
     3781 gcatccggat cctgggccca cggccgcctc gcaatgccga ggcgccgcgc ccgttgcctg
     3841 ccggcgagaa cgcttcggtc taggttgcga tgctcagagc gctgagctgg tcggtgacgc
     3901 ggctggctgg gcgctacttc gatcgttgcc gaagccagag attttcagat cgcccgcgag
     3961 gatgtcgcca acgaccgcgc cgagcatcga cccttcgact gcctccatga cctcggtctg
     4021 acctgtccgt cccatgaagt tgaacggggt tgcgttcacg aagtgacagg gcgacgtgca
     4081 ctcgaactcg atttggtttt cagggccgcc ctcgaaagcg acccaggtgt gccgatcgcc
     4141 cttcagtcca aggtatcgaa cggtcaggag aggtttcgtc gagatcccgt tctgtcgatc
     4201 ctgttcgctc aaggccgggc ggtaaccata gatcccgtcg ctttcataga cgtatgcgtg
     4261 gtgacggggc ggcggtgcgg ctggagcccg gacttcggcc acgtcaaccg gcgttgatcg
     4321 ctcgcgaggg atccggtcgt ccatcgcgac gaatgcgccg gcggcgacga tgcccacggc
     4381 gccgagcgca atcagaccat agatcgcttc tcgaatcttt cgccaccgat ccgacttgtc
     4441 catcgactga cctcccgctc tgtgacggga atggtagcaa accgttgagc agcacgcgaa
     4501 gcggccggtt ggctcggctc tcgcgaggct gccggttctc ggcgacgccg ccacttcgac
     4561 caatgagatg aattcgcttg acgcggctga gtttgatctg ttttaatacc gccggtactt
     4621 cggtactaat cccgcgagag gagatcgaat gcacaacatc gtagcccgcg aacgggtcgt
     4681 ccaggccttc accgaagcgc tggacgccaa ccccacgcac cacaccatcg acatcgccca
     4741 ggacgtggct gaccgcctgg gcatccccgt cgaatccgtc ttcgagtgcg tcgacgcggc
     4801 tcccgagccg gccgccgcct gatcgtttcg gccagcgcgg cggcgcgcct cctcctccct
     4861 ccttcccttg tttgccgccg ccggcgcctt cgggcgccag ccaccttctc acctgaacgc
     4921 cgttgcggcg cgtggagctt gcgattgggc cggctttgaa gcgcgtcgac cgaggatcac
     4981 ccggcctgcc tcccgggccc tcggtgcagt tgaggccgcg gccgcaattc gcggcgccgg
     5041 aagcacgtaa ccggcaccta cgcaccaacc aggaaccttc catgaccaac acgacccctg
     5101 tgaccgacgg cgaactgccg aaccgcgggc cgcgcgtgaa gccggcagac gtcgaggcgg
     5161 agatcgccag cgagcactac ttcgtcgccg gcgatggcgt ctacggcgcc gcgcacaagg
     5221 ccggcgacca ctccgtgcag gcgttcccgc cgtcgctcga cctgctgacg ttctgcgtgc
     5281 tcacgctgcg caacggcttc acggtcaccg gcgagtcggc gtgcgccgcg ccggagaact
     5341 tcgacgccga gatcggcaag cgcatcgcgc gccaggccgc ggtggcgaag gtctggccgc
     5401 tgctcggctt tcgcctgcgc gacgcgatcg cgaccgcctg accggacacc ctgtggaggg
     5461 tcgtgatcgg ctgcaaggcc gacagagggg tgcaagtccc tgatgcacgc ggaaaccccg
     5521 cctctgcgag gccgaaggcc cggccgggtg gaacgcccgg cacccatcac caaccgctca
     5581 acgaaaggag cactctcgat gaccaccacc acgaacgcgc ccaccgcctg gaagaagggc
     5641 gaccgcgtca ccgtcaagac ctcgcgcggc gcgccggcga ccggcaaggt gctcgcgcgc
     5701 gcggccgagg gcctgaaggg cctgtgggtc gccgtgaagc tggatgacgg ccgcgagatc
     5761 aaggcgcgcc cgggctcgct caccgccgcc tgatcgtgac cgccgcggcc cgcccgcggc
     5821 gcccttccct cccccacgga ccgaacgcca tgaccgagac caagcacctc gaccccgtca
     5881 tcgccgcggc gtatcgccgc ttcctcgaga tctaccaggt cgacatgacc gacccgcacg
     5941 tccagccgac ggcgagcctc tcgcgcgggt tcttcgcgca cggctacgcc gaggcgcacc
     6001 gtgccgccat cgcgcagcag caggaggacg cctggcgccc gatgctggtc gagccgatgc
     6061 cgggccgctg gtgcatcagc cgctggaacc cctcgggctg gcgcgagtcg tgggatgccg
     6121 gcaccggccg gtggggcccg ttctccgtcg agctcgtgtc gcgcgagcag gcgctcgagc
     6181 agatgcggcg cctgggctcc gcgccggccg tcgtcgacgt gcgcgatccg gtcgtcccgg
     6241 cctgacgcca tggcgatccc cgacctgccg ccaccgatgt ccgacggacg ctttcgcttc
     6301 ctgctacgcc aaggcacgcg cgaggagttc ggccgcgtgg tcgaagccgt tgcgcgcgcg
     6361 ccgctcgtcg ctctcgtcgc gacgctgcgc gctgagctgc gccgcaacgc gctgatgggc
     6421 ctggccagca gcggcgaggc gatcgaggcg gccggcgagg tcgaacgctt gcgcgatgcc
     6481 ctgggcgaga tccgtccgat cgccgacgcc atcgccaagg gccgctacgg catcgccgac
     6541 gccgcggcca ccggcatccg aaacatcgtc gaccgggcgc tcgcgccggc cgagtcggca
     6601 cccatccccg aaggaacctc tcgtgtctga acccaccacc tcccgcaagc cccgcggctt
     6661 cgccgccctg tcgcccgagc gtcgccgcga gatcgcatca atgggcggcc gcacggctca
     6721 cgccgccggc accgcccacc agttcacgtc ggacgaggcg cgcgccgcgg tcgcgaagcg
     6781 ccaccgcaag ccgagcgcgg cgaatgtctg accagcgcgc actcgtcgag gacgccgcgc
     6841 gcgcggcagg ccttcagccg gcccactggt cggacgacgg ccgcttcctg ctgctggtcg
     6901 gtgtgcagta cccgtggaat ccggtcatcg acaacggcga ctgtgcggcc ctggaagccg
     6961 cctgctcgat cgacgtcaac tggtacgacg agcgcgtgat cgccagcatc ggcctcgaca
     7021 tcgtcgacgg catcgacgcc gacgagttct acgtcacgca cgccgacgac aggcaggctg
     7081 cccgccgcct cgcgtcgacg cgcgccgctg cggccgcctg gcgcgacgcg gcatccaccg
     7141 tccgagaagg cagctcttca tgaccaagca agaagtcccg cagctcgtgc ccgtgggcac
     7201 cgtgaaagtc cgccacgtcc gcggccccga gcgcaggtac accatcacga acggcgcgct
     7261 cgacaacctg gccacgggca cctaccacct gttcgcgtcg acaggcgccg tggccgccga
     7321 tgcgcgcgag ctgaccgtct acctcgacga agagatgtcc gcgctgctgc gccagacgat
     7381 cggtgacgac ggagacgcgc acgcgatcaa gctgcacatc ggcgacggcc acagcggatg
     7441 gggactctac gtgtcgctgg ccgactaccc cgaggaaggc gctatccagc tcgcggcgat
     7501 acggagcccc gacgcggccg gcgagccgca gggagagccc aagtgaccac ccccaccctc
     7561 ccgcccgaag tggcgcggct cgtccactca aaagacctct acgagctgtt gatgaacgtc
     7621 cagaacggca tcgacgcgcc aggtcacgcc agcatggcga atggcgtgat cgtgatggtg
     7681 gacaaggctc gcaacgccct cgcccacctc agcgccagcg cagcagcgca gggagaggcg
     7741 gtggcgtggc aggtctggtg gggtatcggc gaaatgcgcc cgcattggcc gccgttcaag
     7801 accgagaacg aggcgcgcga ccacgcgtcg atgatcagga gcaacaccga ggtgcggccg
     7861 ctctacaccc accccgcacc ccctgccagc gccagcgctc cccgcgcgca ggaggcgcag
     7921 cagccggcgg cctggatcgc gtggccagga ggagagccgc cggtcgaagg tcacgtcgtc
     7981 gtgcattacc ggctgcgcag cggcgcggaa tcgaacaaca gcgccgatgg cttgttctgg
     8041 catcacgacg gtggtgagga cgacatcgtc gcctatcgac gcgcccaccc cgcgcatgaa
     8101 ggcggcgagg atggggagct gccggagctg agcgacgagc acatcctcgc gctgtggcgc
     8161 gaggccactt cgggtaatca cggcgacacg acacatcagt tcgtctgctg gttcgcccgc
     8221 gcaatccacg cctcccgcgc ccagcctgcg gccgcctcgg cagcggtgag ggaggcgctg
     8281 cgaatcggcc gatcatggat aaaggcggtg acgaagagta gccacgcaac cgcctcagaa
     8341 cgagacgccg cactcgtcga ccttgctttc atcgaccgta ccgtcgaccg cctcgccgcc
     8401 gctcccgatg tcccgcctgc ggaggtcgcc caggccgcgc cgctgatggc gaacggcatg
     8461 acggaagccg aaacgagcgc gaccgcgagc gtggtggggc tgtcccaggc cgcgccggtc
     8521 gatgcgctgc cggagctgcc tccactccct tggaaggttc cgaccgcagc ggctggagct
     8581 gttttcaagt acgccgagga ctacgcccgc gccgcccagg ccctcgtccg cgccacaccc
     8641 gcgcccaggg tggaatcgca ggccatcgac gcgaagatcg gtctgcgaga gctgcctccg
     8701 atccggatgg ggtcgcaaga ggtcgaatcc ctggaggctg ctgcgaagga gcgcggcgtc
     8761 atcttgcagg cccatgtgcg tgacctgctg cgagcgccca gggtggaggc gaagccgctg
     8821 acggaggagc agatccgcga gggttaccgc gaggtgcgcg acgcaggcct cgttgtgaat
     8881 ttcgcgcagg gcgtcaagta tgccgagcgc ttccacggcg tcggcacgca ccccgaggcg
     8941 gaaggaggtg cgtcttgagc caaggcatcg ccgccggaaa gtcactgtgc cttccgtttc
     9001 ccctgatgcc tgcattcgac gtgcagatcg tcgttccgcg cgacatgacc cgaggcgagg
     9061 ccgaggggct gtgcgcgttc gtcatgtcac tggctgctcc tgaagcggcc gccatcaaac
     9121 acgccgaggc ggacagcgaa aaggaagcac gatgagcaag caatccgagg ccaaagagcg
     9181 tcagtgctat accccgaagc tcattccgag cgtctgcggc aactgcgccc acaagcgcag
     9241 cacgatcacc gagcatccgg ccgaattctc gtggtcgaag ccctacacca ccgagaagga
     9301 cttgcgctgc gcgatcggcg gcttcgcggt gaggaagatg ggctcgtgct ccgaatgggc
     9361 cggcacgagt gagcaccccg aggcggacaa ggagaagagc aatggcagcc tgtgattacc
     9421 gatcatgcga tgtctgcggc ggcaaggcgt tctacgacgc cgagctcgcc tacgaatcca
     9481 tgtccgacga gaactccatt cgacgtgggg caacgccgat gcgctacgcc ggtcgcgagc
     9541 agttcagcga acccgagctg accgccaagt tcggtcaagt ccttggctac gtcggagact
     9601 gggccgtcct ctgcaccgcg tgcagcaaga cgcatcgaac cgccattctt ccgcgcgagt
     9661 cggccaccgg cacgcaggcg gacagcgggg tggcgggatc gtgacggcct actacaacga
     9721 gatcgacccc tacgccgccg aatggctgcg caacctgatt gccgccggcc acatcgcgcc
     9781 aggcgaagtc gatcaacgga gtatcgagga tgtccacccc gacgacctgc gtggattcac
     9841 gcagtgccat ttcttcgccg gcatcggcgt ctggagctac gcgcttcggc gcgccggctg
     9901 ggcagacgac cgacctgttt ggactggttc ctgtccgtgc caacctttca gcgcggcagg
     9961 ccaaggcgct gggtttgatg acgagcggca cctatggccg gctttcttcc acctcatccg
    10021 cgagcgacgc cctgcaaaga tcattggaga gcaggttgcg agcaaggacg cagagccttg
    10081 gctcgacctt gttcaagatg acctggaagg cgtgggttac gccttcgggg cggtgccgtt
    10141 cccgtctgcg ggcgtcggtg ctccgcacat ccgcgaccgc ctgtattggg tggccgacgc
    10201 ccagcgcaca gatcgtcgac gccaagccgc ggccgccgat tatgcagggt cgcaaaccga
    10261 gcgatccgca aatcgggctg gcggacgtag ccgtacatct gtgctcttgg gcgacacctc
    10321 aagcgttcga cgcgacgaac cggagcacgc cggagcagtt tgtcgcgaga aagctcaagc
    10381 accaggcgac tgccgcggtg acggagctgg ccccgcaggc tcaattatcg agctggccat
    10441 cgccgagaga gtcggcccgg ttaacggctt ctggcgagct gctgactgga tcgcatgcag
    10501 ggatgaccgc tggcggccag ttgaacccgg cacattcccg ctggctcatg gggctcccgc
    10561 cagagtggga cgcctgcgcg cctacggcaa cgcgatcaac gccgaagcgg cgcgcgtctt
    10621 catcgaagcc ttcatgacgg agcccaaccc cctccccgga gaccaccatg gcacctgacc
    10681 cgaccaagcc cgatgcggcg gcactggcgg cggcacgcca ggatttgcac cgcaagttcc
    10741 gtgagctggg ctacacgacg aatgcgcaga ccagtgagct gctggatgtc gtgttcgacc
    10801 gcctcgccgc catggcccag cgccccgatt ccgctggatc gctggtgggt gatcgcgacc
    10861 tcgccctggg cctgcttcgc ggtctgcacc ccgagatcac ccgcgacgag cccatggaag
    10921 tcgcgcagca gattttcgac cacgtccagg ccgagcggga agtagcatcc cggcggatcg
    10981 tcagaatgca agagacgatc gacgcgctgc tgcgccagct acgcggcgcg ccttcccaag
    11041 tcgccaccgc cagcgcacaa agcgccaagc gcgacgccac ctcccaggca gcgcagcggg
    11101 aggagacgtg cgaatggtgg caagacggca acgaggacgg cacttggttc tcggcctgcg
    11161 gcggcgaccc gtggcggttc gaggccgaag acccgaccaa gaacggcatg cgcttctgca
    11221 tccactgcgg caagcgcatg gtgcagcgcg acgccgctcg cgctgctggt gcgcagggag
    11281 gggagcgtgt ctgaccgcga cctcaacatc accgcggtct tcagcgactg cggccgcttc
    11341 cgccacatcc tgcgggcctg ctgggacagc agtcgaccga cgctcgggtg gatctgcctc
    11401 aaccccagtc aggccgggcg cccgggcggc gacggcttcg tcgtgggcga cccctcagtt
    11461 cggaagatgg ccggcttcag cgcggacaac ggatacggcg gcctcgtact ggcgaacctg
    11521 ttcgacttca tcgccaccga cccgcgcgag atgaaggccg ccggctatcc gcgcagcccc
    11581 aacgccgacg actggatccg cacggcggct cgggaggcag gcggccatgt cgtgtgcgcc
    11641 tggggcacga acgcccgcaa gctgtcgcgg cccaccgagg tgctcgcgct cctgcgctcc
    11701 gccggcgcgc gcacgatggc actgcgcctc accgacgacg gcatcccctg gcacccgctg
    11761 atgctgccgt actcgtgcaa gctcgtcgac ctcgaggtga gaggatgatc gcgctcgggt
    11821 tcctcctgct cgtggccgtc attcttggcg tcctgcgcct gggagcgagc gaggcgccgc
    11881 cgccggacta cggcgactac cggggcgacc cgaacggaga tgactcggca tggaagtgat
    11941 cgaaagctgg gtacagcttc cgccccagcg gcgcgtcatt ccttcaacga ctcatgttcg
    12001 cccttatgca cgggcaggtg gtgcactcgt cgagaaggat gcgcgcgcta ttgcacgtaa
    12061 gcaatcgacc agcctctgag cgcctgtgct cgaatagagc caaggatgca gttgttccaa
    12121 tcgcttaatc gggaactcta gacgctcacg gctagcatga tgacttaggt tgcgggcgtt
    12181 gaagtggccg gcgcagcctg ctcaacccat aggcggcgtc cacggagctc ctcaccgtca
    12241 gccacaacaa ccacgtccag ataacggcag tcggccggaa gctccaacgg gattattgtg
    12301 acctgaagca ttatttgcgc tctggtcctg ccttctttat ggatgtcttc tactttcgga
    12361 ttcagcgtac cgctttcgaa aatgacctcc ccatcgtttc gctcgattcg agcactgaac
    12421 tccgagacgg gacgatcaag cggcgtatca atgcaaatcc acacgcaaag tcgcggcaac
    12481 cctaacggaa ggtgagggac actcatccct cccgtgtaga cacccatgaa ggatggctta
    12541 tcgccaacct caagtcgaat gtcgtcgcac caaagattgg taacgaatcg gctcatacgt
    12601 cttctctctc attaagcgcg gcccgagaat tttcccatgc actcgcaaac tcatccattg
    12661 aaacgccaag cgcttgcgaa atcctacgaa tttgcatcag gtgaggctcg tgcttcccag
    12721 actccattct agaaatatga ggctgctcca ttccgcaagc ctcggcaagc tgtcgctgcg
    12781 acatcccggc tcgcagacgc aaagccgaca gcgtcatagt tggatagaac gcctccgcaa
    12841 cccatgcccg cgcccgaggg agctcgctgg cgtaaacagg gtcagcggcc agctcatcga
    12901 gtagttggtc gaactcaggg ctctcgattt cagaattatt ttcgggctcg acgataaacc
    12961 tatacgtttg tgcagcgaca cctttcgcaa atccatctgc ttggcctgca gcaaaggctg
    13021 catcgcacgc cagtctagcg acgaaggaac tcgcgctgga tggtaccttc aattcgcttt
    13081 cggagcgggt gatcagggtc gtcatagtca aactcacttc tgcgcgcaat tccaagaatg
    13141 tggaagcact cggatcgcga attaaatata tagaatattc gaaacctcag ttcgtctcgt
    13201 tcaagctcgt acgacttaac tcggtatatc gtgcggccca agcggagcat gctgccccat
    13261 ttttcgaccg atatgctctc tgtccagtct cgcccataac cagcttgcaa taaacgacct
    13321 tgcaactctg cgtctttgcg caatgcatcc acgaactccg ctagacgggc gaagtcccga
    13381 ggcgccatct ggcccctcag gactctcagg tctttcacag cctctccgtg aaccttcagc
    13441 atgtatgata ccacccttga tatggtagcg cattcccctg atcgggggaa cagcgccact
    13501 cgacagcgcc atgttacagg gtgtatccgt cgcgagccct gccggccggc cagcttcagg
    13561 ccgccaaccg gtgctcgtag tagggtcgtt ctcgatcgtc caggatcgcc tgcatagcag
    13621 ccacgtcggc cccaggccgg agccacgctt cgacgttctc ccgcttgatc gggatgatgc
    13681 accggtcgtg cccagcggcc gcgacctccg gcggcggctc gtccgtgatc gcagcgaacg
    13741 acagcaagtc agcctgcccg ggcgccgtcc agcgggccca gaggcaggcg acgagcatct
    13801 cgtcgaggcc cgcgggcttg aactcgagga tcatgtcctc ttcggcctca ccggcggcga
    13861 ggtcgcggcc ctcggccttg tgccgcggca cgtgctcgaa gaacgaactg gccagcatga
    13921 tcccgtgcga gaagccaaag agcggcttcc agaagcggct gccgaggctg tcgcggcgcg
    13981 cgttgtaggt gccagggaac ttgacgtcga agaaagccgg cgagccgcac ggccggcact
    14041 ggtagcgcat cggcatgacg acgaactgcc cgtcctgcac gaccatcacc ggcgcataga
    14101 cgcccgggaa gatccgagag tcgcgcggct caagctcgct gcgctggagg tcggccagcg
    14161 cccggcgcgc gcgctcgatc ttgtcggggg caatgcgcgc gtcgtcggca gcctttttcg
    14221 tcggcttggc gccggccaga acgcgctccg cgtccgccag gcgcttccgt tgggcgaaca
    14281 gctcttgctc gagcctcgtg gcttcgctgg cgtgccatgc gtcgatctcg gccttgatcc
    14341 gccgctcgtc gtcggtctga ggatcggcga aggcatgctc catcgccttc gggatcttca
    14401 ccttcgccgc gggctcgtgg gctcgctgcc agaaggtgcg atagaactcg ccgacgctca
    14461 tcgtggcgcc gaacatcctg gtgtatttcc gccagtcggc gtagacctgg gcgctgtagc
    14521 acatggcaat cgccctcagg gctcgaccgg gatccgcagc tcgtcgagta cagcatcgac
    14581 gccctgctgc gcccacggcg ttcgatgctc aggcgagagc ccgcgaccgc actccgagtc
    14641 gaggctccgc agcaggatcc ttcgagcctc gtccggatcg gggaatgcgt tcacgagggc
    14701 gcgcacggcc gcggtgagcg catccagccg gccgaagatc tcgtgcatgt cctgggtgtc
    14761 tttctcgccc aggttggtca tcggggttgt gtccatgtcg tcgcacctcc gagaccgatt
    14821 ctgcacctcc tgtcgcgcga caatggatca tgaatttcgc ctcccgcctc ctgctcctcg
    14881 ccgcgatcgc cgccgccgcc acgcccgccg acgcgagccg ccgcaagcat tcgctggtcg
    14941 accaagccgt ctctgcctac gaggacagcc actcctacag catccccgcc gcaggcgcca
    15001 tcgaagtcgc cttcagcccg aatgagggct cggagcatct cgtcgtgaag gtcatcgaca
    15061 gcgcgcagcg cgagctgcgc gtcctcgcct attcgttcac gtccgtcccg gtcaccgaag
    15121 cgctggtgcg cgcccggcac cgcggcgtcg acgtccgcgt gattgccgac caaaaggaga
    15181 acgtcagcca ggaccgcagc ggcaaggctc gggccgctct cggtgccctg caggcggccg
    15241 gcgccgacgt ccgcacgatc agcgtctacc cgatccatca cgacaaggtc gtgatcgccg
    15301 acggccagac cgtcgagctc ggctcgttca actactctgc cgcggccgcg cacgccaaca
    15361 gcgagaacgt cttcgtgaac tgggcgaacc cgaagctcgc gagcgtctac ctcgagcact
    15421 tcgcgaggaa ctacgctcag gcccagccgt tcgtcccgag ctactgaccc gcggcgccgc
    15481 gcgagcgcgt acgatcgcct tcacccaatc tcgagacgac gcgatgtcga ccttcggcgc
    15541 ccgcctgaag caagcccgcc agctggcagg gctctcgcag cggcaactcg gaatcctggc
    15601 gggcatcgct ccaggctcgt ccagcgcacg gatgaaccag tacgaacgag acacgcacga
    15661 ggctcccatg gcgctcgttc gcaagctcgc gcgcgtgctc gacgtgccgg tgccgttctt
    15721 ctacgccgag aaggacgagc atgcgcggct gttgctcgcc tactcgaagc tgcccgctgc
    15781 gcaacgccgg gccttccttg cgagcgtgaa gcaggctgcg caacagctct cgtcgagcaa
    15841 agcctgacgg ctggatcgcc gtcgacacgt cggcatcggc atcagggtga gcttggcggc
    15901 gccgacgcgg ctgcgtcctg gccgaccagc gcgagtcgca cgctggcctt gcgcctcagg
    15961 gcgtcgtact cggcttcgca ggcgaggccg cggaggtggg cctggtcaag agctgcctcg
    16021 agcgttcgcg cgcggccgtc ccattcgtcg aacacgtcgg ctcgcagatc gacgggagcg
    16081 gaagctgccg cgctgacgga gacaggtccg ggatcaccgc ctcctgcacg accaccggcg
    16141 gcgacgccgg cgcggaatgc gttgcgcatc cgacgatcga catcggcagc agcgagagag
    16201 tcagcatcgc gctgacggag ggtttcgagg cgggtcgctt cacggtcgat ctcctggttg
    16261 gcttggatcc gacgcccgct ctcggcgagg cggtctgcgg tggcactcgc agccacggtg
    16321 gcggcggtgg cgcggtcggc ggctcggccg gccttctcgg tcgcaacggc ggtgcgcagc
    16381 gacccgatgc ggtactgctg gacgccgagc aatccggcga tcgtgcccac gacggcgagg
    16441 aggatcgcga gctcggccca gcgcacggcc ggcacctggt tggcgatcca cgcaaggatg
    16501 gtcttcatcg aggtctcctt cagagcttgg cgcgcaccgg caggatcgcg gcgagcgtca
    16561 gggtgtcgcc cgccgaggtc gtgaccgtgc actggatgcg gtagtcggtg ccgtcgacgc
    16621 cgccgacgac gcgctgcagc acacgggccg cgtttccgct gtcgatctgc gggtcgccgg
    16681 acaacaccga gagcggcgag gcatcctcgg caccggactc gacctcgcac gtgaacgagg
    16741 gggcggcgat cgtggccgtg ccgattgcca gcgagaagtc gtaggcgaga acgaccgact
    16801 cgagggtgcc cttggtcggg aatttcgtgg tcatgaggtt gcactccgag aagttgtggc
    16861 cgcctgcgca cgccaggcgc gcagagggag gtcggcgatc caggcgcggg ccggcgcgac
    16921 aacgtcgaac ccaggcgtcg cagacagcgg cggccatgcg gtagccagcc acgctctcgg
    16981 cgcgagcgag gcgacccaga tccgaggcat ggcgttggct ccccacgcgc gcggctgcat
    17041 ggagaccgcg atcggcggga tcgatcggac tgcgccggcg gccgcccagc cccgccgcgg
    17101 cacagttgcg cgccatgcac gcagcgaagc gacagcgcgc cagtcacggg gacgagcaga
    17161 cgcactgaaa ccgggcgtct gcgcgagcgt ctcgccggcg ccgcccgcga agaggaggct
    17221 gcgcccgaga agcagcaggc ggcggagagc gttcatgcct gcacgagccc cgagtcggtg
    17281 ggctgggtgg taccggtgac ggccttcgga ggaatgagat ccgtcgtcgt gcctcgggcg
    17341 taggtgtgca gcaggccggc ctcgtgatcg tagacctcgt cggcctcggc cagcgcgcgg
    17401 atcgcggcga catcgtccgt gatcgatcct ccgacgtcga caacggtcgc gttggccgac
    17461 tgcacgagca tgacctgcac gcccgggctg taggcgagcg ggtcatcgcc tgggccgccc
    17521 acgaggttgc cgcccgacac tcgggcctgg tacgcgccgg cggtgaagag gagctgccag
    17581 ttgcccagca gcttcacggt gatgccgacg tagacgcccg acccgagcgg caccaagccg
    17641 ctgcccgaca ccaagggccc gtacagcatc ccctcggcgg actgctgcgc ctgctgcgcc
    17701 gcgcgataca gcgccgtcac gtcgaccgac gtcacgccgg cgtcgacggc gatctgtgac
    17761 gcctcgaagt cgaacgtgaa cggggcgatg cggacggtca tggctcagac gtccgcagtg
    17821 cgcaccgtgt tcaccgatcc gccagccgag agcacgtcga gagtcgtctc gaacggcacg
    17881 atcggggtgc cgcccgatcc ctggcgtacg cggatgcgcg cggtgaactc ggccgagtac
    17941 gtgaagctga ccgattcgct ggtgctggta gtcgtcttgt cgatcagcgg cacgaaggcg
    18001 ttggcgccgt tggcgtgcgc ttcgcccgtc gttcccgaga gggtgaaggt atggcccgtc
    18061 cacgaggtgt aggcgtagcg aacgccctcg acgcggatca cgcccgcgga cgggtgatcc
    18121 gccgtgatgg tctcgttgac ggtgatcgtg ccggagccga tcgcgtgcgc gccgttgagg
    18181 gtgaactcgg tcgtcaggat cccgcccgag ccgttgtcgc gcgccaccag cacgccgtcg
    18241 cccgagacga ggttgccgac ggtgatcgtc gtgacgaccg gcgccgaatg cgtggttccg
    18301 tcgtgcgagg tgagctggta gttctggctg tctgcggcct ggacgcccga gagccaccag
    18361 ccctgcgcga ggaacaactt cccaccggcg aagttgccga acggcgccgc cttgttcggg
    18421 gcataggccg cgttgaggta ctggtactgc tcgccggcga cgccgttgat cgcgccggtc
    18481 tggccctcgc aggtaatggc ctgcaggtac tggtagagct gcgccaccga gatcgatccg
    18541 tccagcgcga tcgtgccctt gtaaagcttc gaaccggccc cgtcaccgat gtcgtggttc
    18601 gagtcggcga cagtgatcgt cacgtgcgac gacagcgcga gcgccgcggc cttcgagagt
    18661 gtgatgttcg cgtccgcgga cgtcccgatg gccgcgatgt tctcgccgcc ggcggtcagg
    18721 tcgacgttga agtagtcgta ggtctggcca tagacgcggg agaaggcggt cacggtgccg
    18781 tctccgatcg tgctgccggc attcgtcacg agtaccatga tctgaacgtg gccgttgggc
    18841 cagaacttcg tcagcttcgt cccgttctgg acgacataga tgctcgaacc cgcgacgatc
    18901 gagccgatgg tcttcaggcc ggagtagagc gtcgccccgc tggcctgctt gatcgagccg
    18961 aagttgatga actgcgccgc cgttgcgtcg atgttgaaag tcgacagcag gttgagccgc
    19021 gaggcgatgg cagcattgcg cgggccgtcc agcttcgacg ggttgaccga cagaatcgac
    19081 acgaggtcgt cgctgctggt ggccgcgttg tccgccatgt cctgaagcca cgcgtgaagc
    19141 gcgaggacgg agtagaccgt cgcgccgccg gtgtgccgga tgttgccgcc tgcatcgacg
    19201 gagaaatcgt cttggatcgc catgttgggc tccgggggtt actggtcgtc gcgcacctga
    19261 agcgcggtga tcgaaaggcc gttgatcgtc ggcgtgcctt gcgcgacgaa gggctggtag
    19321 tagggccccg gcgcagtagc gcatcgcagc tcgatctgca tcggaacgtt cagccagtcg
    19381 ccagtgacgc tgtaggtcga ggccgtgacc gcgtcattga cgagcaccgc attcgtgtcg
    19441 gtgcgcgtga tcttgatgcg cgacccaacg acgcagttgg tcacggtcac ggtcacgggg
    19501 tcgagcgggt agctgacggc ctgctggttg gcgagcgtcg tggtcgtggc gatcgtcagg
    19561 ccggtgatgg ccgtcgtcgt gccggtcgtt gctgtgagcc ggacggccag cttgatgccc
    19621 ttcgacggcg tgatcgcgac ggcctgaagg ttcgtgttgg tggcggcctg ccaggccgac
    19681 caaccattgc cgtcgccaat gtccgcgcgg aattcgaccg tgtagttgcc gatcgtgccg
    19741 cccgacatcg atatcgactg cagttgcgag tgcccctgtg ccgcgtagtt catcgtccag
    19801 actacctgat cgccgacaga cggcatgatg aggccggggg cgttcgagaa ctgcgcggtt
    19861 ccgctatagg agagctggtc actcgtcagg ctcaccacgt ccgtcatgag gatcgcaatg
    19921 gcgcccaccg tgtcggacgt gaagtagtcg gcccaatgga tgcccgacgt gttggccgcc
    19981 gcacgcgggg cattcgtgac gcgcactccg cggaacttag cgttcgaggc cgagaggatg
    20041 gcggccacgc cgggaatcga tccgccttcg atgttgaacg tcgcctgcga cacgagctga
    20101 gtcaccgacg ccgagctgct gttgccgtcc gcgaaccagc agttctgaac gaagaccccg
    20161 aggtcgtacg cgttgttcag cgtcgcaatg acggcaccgc tcatcgggtt gacggtgccg
    20221 aggctcaacg gctgcgcagc cgtcccgaag ttgcgcacct tgaaccccga gctgccagac
    20281 ggcacgacga tcccgttgtt cgacggcgcg caggcgttgg ccgtgttgac gatgtacgtc
    20341 cagccgtcga tcgtcgcatt cgtgaatccg ctaccgctgt tccagaacgc ctgcccggaa
    20401 agcacggtcg cgccgagcgt gccgttcgac cgcgcggcga accgcgagtt ggagagcttc
    20461 gtgtcggtga gcgtcagggt gtagatcatc tcgccgacga agtcattcga gtcggccgag
    20521 gtcatgcttg agaggttgat cgccgcgcaa cccgggttcg atccgctcac tgtgcgcggc
    20581 accattacct ggaagcggtt gcggtccatg tagccgccgc cgttcatcgt caagttgcct
    20641 gcgtagacga acgacgtctg gatcgcgatc gacgagaacg tgttgtcgga gacggttccc
    20701 ccgttgaacg tcgtcacgac gcactgcgtc acgtccgcct gggacgtgtc gcgctggccg
    20761 acgatgttgt tttggaaggt cggcgcagac agcgggttga cgatctggaa tgggccgacg
    20821 atggtgctgt cggtcacgct gatcgaggca tagggcgcgg cgttcatgcc ccaggcgaaa
    20881 ttcgcgaggt cgaattggag cgtgccggag ttgctggccg gcatgaagta gcgcgtgttg
    20941 accgtggaac tgggcgtgct gttcgcgccg aggccgacgg tcgtgttcgt cgtgacgagg
    21001 cacacgttgg gtacccggat gcggcagccg gacgcgggca gcacgccccc gacgttgcct
    21061 ccgccgtcct tcccgatcgt cagcacccct gccgacgaga tccagacgtt gcgctgccgt
    21121 tcgtcggtcg accatcctgc gttcgtcgag tcggcgccgt tgccgtagcg ctgatagacg
    21181 ccggagcccg cgctcgtctc gatccatacg ccgttgtagt aggtgccggt cacgctcgcc
    21241 ggcagttgga tctgctggcc ggcgacaccg ctcgtcttga tctccgcacc cgtcgagtcg
    21301 aacggcgaca gccacgcgcc ctggatggtg aaactgccga gcttgcccat cgagaaggcc
    21361 gacgcttcct tccctgcgac ctcgatccag cccacgatgt cggcgccgcg cgccgtggcc
    21421 gtgatgcccg agagcgcgcc cgcggcgaac gagcctccgg tgacgttcct caccttgatg
    21481 aagccgctcg cgggcatggc cgagcccgcg gtggtgggcg tcgtgttgat cgcgctccag
    21541 acgcccagca gctcggcact cacgccgcct tgcgtgatgc tcgtgccggc ggccggcacg
    21601 ttgccgctgc cgctcgtgta ggggatgagg cgaaccttgg tgccgtcgat cttgacttgg
    21661 ccaccgagcg tggctgagat cgtgattggg cccagcggcc cggtcgtcgg gctcaggttc
    21721 gggccatagc gggtgtcgca gtcgatcgtg agcgcacccc cgttgacgtt gtacgtgtcg
    21781 ccgcctgcct tcccggccag tgccgtcaaa ttcgtgggcg tcgcgatgga aaacgtcgtc
    21841 atcgactcag ctcgtcaagc cctgcaggca gacgccgcgc tcgagctggc ggcgcttggt
    21901 gaggcccggc agcgcgacgc tcacgccagc cacggtggcg cgatcccagc gagggagctg
    21961 ctcgcaggcg ccgcgccatt ggcgggcggc gagcagccgc gccgcggtgc tcttgcggac
    22021 gtcacaggcg atcgtcgggc cgaggttgaa aacggcgtca ccgaaggctg ctgcgacgct
    22081 gtcgggcgcc ccaggcgcgc agcgcagcac cgtgtcgacg gcctggccgg cgtcggcctg
    22141 caggagctgg tagcactcgc cgtcggagta gtagcggccc gcggtcacgt tgccggtgtg
    22201 gccgtcgcag accgtgagga cgccgggcgg gtcgtagtag gcgtagtgcc ggatgccctc
    22261 ggcgggcgcg gccagcgcgc agacggcggc cacgatcgcc gagcgccggc gcaagttgtc
    22321 agcgactgcc atcggcgtct ccctgcagtt tcggctgcgc caccaggcgc gcgccggcgg
    22381 cggccaacga cacggcggcc gtcaacgcgg cgaaagccgg cgaggagtgc gcgggagcga
    22441 tgtacggcag cgccgcatcg gcggcgccga gcagcgccgc gaggatggcc agcctcacag
    22501 accaaagcct gtgccacgtc ttgccagcgt cgtcgatcag cttcatggtg gtctccaacg
    22561 gtcagtgaga acggggcgga ttgccgccac ggaacgtgat gtaggccacc ttgacggccg
    22621 tcgccgcgcc agccagcacg gagaccgcgc gcacgaccgg gcccagccag agccacatgc
    22681 gcgcgaagaa ggcggagacc ttcgccaggc cgcggaagaa gctctgggcg gtctcgacga
    22741 tctcgtggat gtgcccgatc atcggcagcg cctgggccac cgatgcggcg gtcttcgctg
    22801 tctcggccgt attcgccgcc agcgacgcct ccatgccgtc catccgcaca cggccgtgct
    22861 ccagccgctg cagcacgagc tcgcgccatt cgtcctcgga gaggcctttg atctcgacgc
    22921 gcgaaagctc gtggatcgcg ccgaccgacg acatctcgcc gcggctcgga gtctcgtctt
    22981 cgtctcggtc ttgcagggga tcggtcacca ggtgtccttg gagatgtgcc tcagctggtc
    23041 ggccagaggt cggagttcgt gacgtagcca gccgggctgt ggcgggcgat acccttggtg
    23101 atgcgaatgc ggccgatgag tccatcgaac gcacactgcc cagagttcgc ggtgtcgttg
    23161 ccgatgtcga cggcgccttg caccgtccaa tggtgcgaat cggcacgcga cgcgaccatc
    23221 gcaccgttca cgaacagcat cagcgtcccg cccgcgcgct gcagacacat gcggtacagc
    23281 gtcccgatcg aggtcgcgaa cgggaagatc gggaattcgg ggccgcccgc gatcgccagg
    23341 tttcccggcg agaggtcgtg gtggtcgacg atgttcgcac cgtaggtcga gccatccagg
    23401 tacgtcgtca acatcgtcgg atacgcgctg ctcttggcgc gcgctttgta gtcgagctcg
    23461 atggtgaagt cgcccgctcc gaagtcgagc tcagtgccga aggccgtgat gccaaggtac
    23521 ccgcggtctg gactgacatc gatcgcgccg tgcggaaaat caggatccgt cgtgttgacg
    23581 agaggcgttc cgctgcccgt accggcatac gtcagtaccg tgtgcccggc ggcatcggtg
    23641 agcgtgtcgc cgttcatcaa caagccaact gcactgaagt acgggtcgac ctcatgacct
    23701 ggcgccccgg ccaacggcag cgagacgggg tagttgtcgg tgtagaggca cgcgctcgcg
    23761 agtcgaactt cgtcgagctc gaaggtcgtc gcgttcgtac cgggcgcgtt gaggccgccc
    23821 acggtgatga ccgcgcccgc gtgggtgtag tcgttgttgt cgatgaccgt ggttgcgctg
    23881 acctggccgt tgaggaacgc gcgcaccacg ccgtgcgcat cgcgagtgat cgcgacgtcg
    23941 ttccaggcgt tcgcgacgac ctcgaggtca gggaaaggcg cgcgcgtgtc ggtcgcgccg
    24001 tagacaccct ggtaccactg gagttctcct ccgtcgaaca gcacgatcat catccgatcg
    24061 ccgtttacgc cgccagccgc cgagctgaac aggaggtgct tcgcaccgag gtcggtcggc
    24121 ttccacgaga actcgatggt gaaagggccg gccatgctga agccggcggg ccacgaggtg
    24181 gagatgccgt tcgagctcga gaaccgcccc gaggtcgcac ctgcacgggg gtcggcgacc
    24241 aacgtgacgc cagtgtccgc cggcgtgaag gtcaggccac ggatgccgct gtccgtgaag
    24301 gtctgcgacc cggcagtgcc ttcgaagtgc agcaggaaca cagtcgagtc gtccggcgcc
    24361 gcggcgacgg agatgatggc ggccttggtc acgctgcgca cggcaccgtt gagagtcacg
    24421 gtcatcgtca ccgagtatgt gccctgcccg ccgtaggtgt gcgtcgggct ggcgtcggtg
    24481 ctcgtgtggc cgtcgccgaa gtcccagagg tgcgtgtcgg cgcggccgcg cgtcgaggtg
    24541 tccgtgaacg acacggtcag cggggctgtg ccgctggtcg gcgcggcgac gaaatcggga
    24601 cgcaggatca gcgcatagat cgccgacagg aattgggtga cgtccgtcgg atcgaacgtc
    24661 atgcccgcgc tctcgatcgt gctcttgatc tcctcgagca tgtcctcgaa ccactcgccg
    24721 ccaatgtgcg tcgggtgctg tccggtcgca aggtcgcccg cgcgcgggta tccgatcgag
    24781 cgcgtggtga ccggctccca ggcttgcgac gacgcgtccg acttccagag cgggtccatg
    24841 tcctatcccc cgtggtaggc ggcctgggcg gcctgcagga gctcgacatc ggcatcgctc
    24901 agccgcgacg cgtacacgag gatctcgggc gtcgagaagg ccgcggggaa cggcgaggtg
    24961 ttgacggcgt agccgtcgcc ggcgccaccg gagccgatgc ggcagaacgc agcgtcgctg
    25021 cgctcaccgc gggcggcagt gccgctggcc tgcaggctgc cgtcaaggta gacatcgacg
    25081 gcgccggagg tcgaggtgcg tcggcagatg ccgacatgca gggcaccgtc attgatcacc
    25141 gtcgcgccgc ggacacctgt ctcggggccg aggtcgtcgc cggcgccgcc ctcccacaac
    25201 gtgagcgtgt tgccgaggtt gccgaagccg aagtcgcgcg aactgccgga cgtatcggcg
    25261 tagaggaagc cgctgacttg ataccccagg ccggtgctgg tttgtccgtt gctctgcgtt
    25321 gtggcgaaga ccgcgccgag gctgaaatcg aggcgtgcgc ctgccgcgtt gttgaagacg
    25381 aggccattcg cggtgctgaa caaagccgcc gggaacccgc cgatctcgag caccgccccg
    25441 gactccacga tgaggggctg agccgatgca ttcgcctgca cggcatcgat cccgctcccc
    25501 gtctggttgt accagttcgt gatgtacccg ttgcccgagc cgacgaaagc cagcagagcc
    25561 gccgtgtcga cggtgccacg cgcgaagccg atgtccatct ccgagttgtc gctgctgcgg
    25621 cgcacgcgca tgcaggcgcc gccgtagtcg atcagcagca ggcgcgtcga gaacgccatc
    25681 gccggctgca cggacagcgc gtccagcggg gcccgcgccg gcgcactggc cagcgcgacg
    25741 acggcgtcag cgagctggtg caggttcgtc gcgtccggcg tgaggccggc cgcctcgatg
    25801 acccgcatga aggactcggt cacgtagtgg aagatccacg ggcccggcgt ggtcgggggt
    25861 accgtcgcct gcagcggatg cgggacatcg ctctgcacgt ggccgtgcgc cccgggcgac
    25921 ggaggcgtcg gcggtgtggc gctgacgttg gcctggtaga cgcgatccac cctcagctcc
    25981 ccgcgatgga cttgatcgca gcgagcatct gcgtttgcgc gccgatcgcc ggagcctgcc
    26041 cgccgcggat ctgcaggttg cgcacttctt cgcccaccat gtagaaccag tgcgggcccg
    26101 gcacggtcgc tccggacgcc tcgtcggcgt ccttcgggta gcccgtgacc gcactcgccg
    26161 gcagcgccgg cggggtcccg gctgcaccgg agcggtaggc gcgcggctcc atcagctcgc
    26221 cgccgtcggc gccacgtagg cgggcagagc ggcgaggatg gcagcggccg tcggcaatgc
    26281 cccctgcgcg ttgatcagcg cgagggccgc cgaccagacc tcggagcgcc aggcgcgcaa
    26341 ggccttgccg tcggcttggt aggtcggcac cgcgggctcg tcggcgtagg tgacggccgt
    26401 cgccatgctg tcgtagccgt tgctcttcgc cacggcatcg agatgcgcct ggacggcgcg
    26461 cgagagcgtc gcgcgctgct ggtcgatcgt gggcgccggc ggatccgttg ccacgggctt
    26521 tccgtcggcg ccggcgacga tgctcttgcc ggacgcctgc gcggtcatca gcgcgccgta
    26581 gtcgtcgtcg gagatctcca ccgcgtccgt cggcatggcc tcgccatgga tggcgtcaac
    26641 gtagaagccg gaggtcgatt tgctgtagta gcgggtcatg tcacttcccg atgcagagga
    26701 acgcgagcgg cacgttggag gcgctgtcgg actggacgtc cgccgtcagg agaacggtcg
    26761 acagcgaccc gggcgtggcg taggccgacc acgcgcccgc gacggtgccg ctcgtgtcca
    26821 cggtcgcgaa cgaccgcagg tgagctgtcg ggaaggcgat cggcagcgac accgtgtagg
    26881 ccgtgtccgt ggtcgcggcc aggtaggtgc cccactggac gatcaggccc ccgggcagct
    26941 tctggtagcc ggacgtccca agcgactggc ccgaggtaaa cgcagccgcc agcgtcgatg
    27001 cgaagtcgct cgtgagcagg aagttgggga cgcccgtgat gtccgcgaac gggatggagc
    27061 cctgctgcac gaccgtccag ttggcgccgc cggtgtcggg gtcgctcgtg ttgttgtcga
    27121 ccgtcgactt ccagaagtgc gtgccgtccg cgctcaccag caccgcgccg gcggggtagc
    27181 cgccgatcgc ggtgctgacg cccgcgtcgt agcccggaaa gccgcccgcc tgctgccagc
    27241 ggatccactg cgtgatccac cgcaggatgc cgttgaagtc ctggcccttg ggcggcacgc
    27301 cgccggcggt cgtggggtcg aggaagttca gcggcgggaa gccgtcggtg aggctcgcgg
    27361 cccccggctc gctgccgatc tgcgacggcg tggggatgtc gcggacgtag cccgcgccgg
    27421 cgccgttggc ccaggggatg gagaagaagg cggggacttg actggcctgc atgcgctgcc
    27481 tcgggtcagt ggttgaatgc gccggtggcg aagctgccga cgttctcgcc ggcctcgccg
    27541 aagccgagca cgtggtgcgg gttcaggacg acgacgaaga gctgcacgcc cgaaggcgcc
    27601 gggaacgcgc cagactgctg caggatcgcg aggtcgagcg cggtcggctg gaacgggaag
    27661 ttgatcgccg ccttcatgcc gccgtagtcc acgacgctga cgttgccgcc gcggccggcg
    27721 aacatgagca tcagcgcgcg gttcatcgtg tggatggacc ggtcgctgat gttcgagaag
    27781 gccttcacca ggatcaaacg ccggtagtcg tcgtcggcca acaggaagtt gggcgtgccg
    27841 gtctgcacgg aggcgaacgg ctgctggccg aagccgaaga cgagcgggcc gccggcctcg
    27901 ccgaagccga aggtcttgcc cgactgaccg cccggcacgt acaggccgcg gcgcacgtcg
    27961 acgatccgac cccagacgtc caggccgtag ccgacggcgg tgtcgatgtc ccagaccgag
    28021 tcgaggaaca tctccgtcat cgcgtcggcg tccagcgcca cggcgaacga gctgatcatc
    28081 cccagcagac gcgggctgtt cgcgtactgc tgcagcacgg tgggcggcca cgccggcggc
    28141 acgcgcggat acagctccgc gtcgaccggc atttcgacgg tcgattcgac ggaggggtcg
    28201 aggtagccgg cgggcagcat ggtcaggtga aggtcaccgc gatgtcggag gtcgacagcg
    28261 tcgggatctg gtcggcgcgg agctggatgc tgtcctggtc ggctgcagta atgccgacct
    28321 tgacggactt gagttgcgcc caggcgccga ggctcacgat gccggcgtag aagcgcgccg
    28381 cgaagacctc gccggcaatc cgcgcgcgct cgccgccgtc ggtgccgttg aacgcggcga
    28441 cgacggccgc ctggatctgc gcggccgcgg tcgacggcac cttgccgttg ttctgcatcg
    28501 ccaccgcgac cttgatgccg gtcggcgccg gcacggtgaa cgagatccgg taggtcgggt
    28561 acggcgcggc gaagagcgag ctcgtgtcct gcaccgcgac ggtcgtgttg ccgttcatgt
    28621 cgcagccggg attcttcttc gtgaggatcg ccgcgccgat ggcttgcgcg tcgccgccgt
    28681 aggctgcgac gaagatcgag tgcggcttga gctgcacgcc acccggcgcg caggccatcg
    28741 actcgccggc gaccgtctgc gcgatgttca gcgcgtaggt gccgtcgccg ccgctgccgg
    28801 ccaggtagct gctgatcagc gtcccctcga cgacgccgct cccaagcacc acgtccccga
    28861 cggagatcac gccggccgcg cccgtcaccg tcaggacatt gccggcgatg ctgccggtga
    28921 aggtcgcccc gctggtcgcg tccgacgggt tctcgagcgc ataggcgtcg atgacgccgg
    28981 gcactgccag cacggcggcc cgcacggcgg cggtggtgct gttggcgttc aacgccacgg
    29041 actgctggcg ccggtactcg aagtccgcgg cgctctccgc gtccgacccg acgacgcccg
    29101 cagccacgtt ggcgaccgag tcccatccgg ggatggcctg gtagatccgc tccaggaagc
    29161 cggccgggca cgcgatcggg cccgtcaccg cacaggcgaa cggcagcgtg atcgagccgc
    29221 ccgatggaat cgtgcccgcc tcggtgcaca ggtacaggtt cccgtcctgg tcgaccgcct
    29281 gggcgttgac ggggatcacc gtcccggtct tgccgctgca caccgcctgg acgacggtcg
    29341 actcggccgg gttgcgggtc aggtagtaga tccgggcgat ggcgtcctgc gcgcggccgc
    29401 tggaggtggc cgggtcgact ccggccatga ccgccatgaa cgtcgcatag gcgcccgcga
    29461 tgatcgcggc ttcgctcgtg gcgagctgcc cctgcggcgt gttgagcgcc gggttcagcg
    29521 tcgtgccgaa ggcggcgttg atgtccgcct gccggccggc gaggatgtct gcgtcgtcgg
    29581 gcgcgatcca gccgttgtcg ccgatggtca gggcggggac ttgcgtgctg ctcatgcggg
    29641 cccgatcaga tggtcgccga cgacgtcgcg ccgctggtgc tcgtgacctg cacctgccct
    29701 tgcacgacac ggcccgcgat gccggtgatg aacacctcgg ccgaggcgac ctcgggcacg
    29761 gtcagagctg cggcctggag cagcgacttg acgagtgccg tcgacggcga ctggcccagg
    29821 atctcctggc gcagcggcac gccgatggtc gtgtcgtaga agcattcgcc ctggaaggtc
    29881 ttgatcgccg aggcggcgtc ctgcgcgagg gcgtacggct ccgccgcaag ggcgatgttg
    29941 ccgtcggccg agatgtcgag atcccaggtc gtcgggttga ggtagacggt cgcggccatc
    30001 aggtcacccc cagcgtgtgt ccgccgccgt tcaggttgtg cgtgtgcggc gcgccgatgc
    30061 tcttgccgcc ctgcttgatg tcgcctgcag acatcgtgcc ggttacgttc aggtcgccaa
    30121 tgatcgaggt cgtctccgca gccagggtga tggcctccgt cgcattgacc gtcaccgtgt
    30181 ccgcggcgac cacgaccgca ctcgcgtcga tggtcacggc gggtgcctgc agcgtcacgg
    30241 cgttgggcga cgacagcgtg atgcccgcgt tcgagaactg gacgtactgc tcgggcgtcc
    30301 cgttcagcac gccgccgatg tagagaccgt cggccatcga gtgcgtgcgc cggctgcccg
    30361 gattcgcggg ccccttgttc acgatgacgc tggagatgtc gcgcgatgcg aagacggcaa
    30421 tgccgatgtc gccgacctgc ggatcgagga tcacggcgtt ggcgccgccc tgcaggcgca
    30481 tgtacgggca gccgtagatc gtgccgtgcg cctgagcgac gccgaagccg tcgacctgcg
    30541 ccaccagcgg caggatgtcg accgttccga ccggcgacac gccgccgtcg ttgctgaccg
    30601 cttcgacgcg cacgagggtc gcggtggcga ccccggctag agcctggcga acaagaaagg
    30661 ccagcgcgtt gaaatggctg gccggatcgg agttctgttg tgcgccgagg tgacctggcg
    30721 cgctagacga cgggggcata gccgggcttg gcaacctgca tgagggtgag ccattttccg
    30781 gagggaacct cggcctcaat gtcatgggtc agcatcgcaa ctacccagcg accgcaagcc
    30841 ggcgtcaggc tactctcgac atcgatctgg ccgccatgcc tgaccaccgg attgaaggtc
    30901 gtcgtgacga tgaggcccgt cgacgtgtag gtcgggtacc cgaccatgcc cgtgtccttg
    30961 gcgatgcgcg gtacgagccc tccgcgggag ccgccccgtg gccagatggc gagcacgcca
    31021 ttttcgatgc cattccactc gatgccggca tgctggacaa ccgcgagggc ctgttctcgc
    31081 agcgtgcctg ggtaatacgg tgtcgagagg atcacgctca cgccgttgtt ctcgaaggcg
    31141 acatcgtcca tcctgtcggc aatgttcgag aggacgactg ctgcgtccgc ggtaccgggg
    31201 tagctgagcg gcggagaacg acgcagcgcg tcgatcaagc ccgcgaaggc ggtgatgttg
    31261 aacgccacct ccggtgcagc ctgcatgtcc gcccaagcct cctggatagt gccctcgaag
    31321 atcttcgcca tgccgatgac gtcatcgccc gcgtacaccg agatcgtgtt cgcgcgatag
    31381 gcattgggga gctgccccat cgtcgagagc tggttcatca tcgagagcgt cagcccgaag
    31441 atgcgaacat gcgcagtcgg catgccggct ccgcccgcgt tcgccacgtt cacgctcaca
    31501 cgatgacctg acagggtcac gctttgcggc ggcccgtcgc cagacgggcc agtcccatac
    31561 gcgaactgca gcgtgatctt gcgcctgacg agcgccatca ggcgagcgcc gcgaagtccg
    31621 cggcctccac gtagagcagg ataaagcgac tgccgatcgt cggctcgctc gccgagtaaa
    31681 acgggtcgga ttccgccgcg gtgtcgatca tcatcaggtc gccgacaaag tcgaagtagg
    31741 catcgcgcag caccagtacg cgatcgcgga gcatcgcgcc accgaggatc agcgcgtcgt
    31801 cgacgtagac gtcgatgaac atgccggtcg tgcgctggta gaccttcagg cggcacgact
    31861 ggccgccaag gagcgccgac acggtctgag atggcagcgc ggacaacggg atgttcatca
    31921 tcgtagttgc cccttcaagg cgaggagcgg caggctcgcc tgacgcttca gcgctgcgtc
    31981 tgcgatggcc ttctgcggcg ccggcgtcgc aactggctgt accgtcccgc cgaccacggg
    32041 tgatgcggcc gatggactgg cgacctgcgt gaatgccgcg actgcggtcg tgcgaatctg
    32101 ccgcaggcca atgtcgacga tgagcaatgt cgctccgtct cgcgctgatc tgccgtaccc
    32161 gaggcgcgag acgttcacgg acaggtacgt ccgctcgggt gtcacgacgt cgtaggtgtt
    32221 aagcgattcg gcgagcgcct cgacctgcgc aaggaaggct gtccggtcgg actggctacc
    32281 acccttggtg atccgaagcc gggtgtcgaa aggacgagcc accttgttga acgtctcgaa
    32341 tgcgccctgt tcaacctggt agtccagaag cgaccactcg cgctggtagt ccatcgcgac
    32401 gatcgagtcc ggctcaagcg ccttggagcc gttgaagaag atgccccact cggccgactt
    32461 tgcgcccgtg ctgcgcgcgg tgtccgtggt gtcacgggcg gcagccggag cgctcatgcc
    32521 gctcagcagg gttggagcgc cgtcagccat cagaacatcc ccgtgttcgc ctgcgctgcg
    32581 atcagcgctg gctgcatgtc tcgcgcgatg ccgcgcgcat cggtggcctg tgtcacgatg
    32641 gtcatgttct cgatcgtcgt ctcgttgttg gtgcctgaac ctccgctgcc gcgcagacgg
    32701 cccatgatgt cgcgcacata cttctgcgtc tccagcggca gcgcgccgcc ggagcgctgg
    32761 aggttgccgg agccgaagtt gtaagccgct agcgtcgtcg gcaggttgcc gccgtactga
    32821 cgcagcaggt cggccagcat gcgcgccgcg gcgctggcgg actgctgaaa gttcatcggg
    32881 tccgttatgc cgtatgccct cgctgtggcc ggcatctgtt ggaagtcgcc catcgccccc
    32941 ttcgggctga ccatgtgctg cccgcgtccg gactcttcct gccataccga atcgaggagg
    33001 cccgccggaa gcccccactt ctgctcgagc gatcgaaggt attcctgctt cgagccgctg
    33061 tcgcccgtcg tcggcaccgc cgagccaccg gctgcgttca gttgcgcgag tcgcgccgcc
    33121 gcgtcgggag tcagcttcgc tccctgacga tgttccacgt cccaccattc cttgagcagc
    33181 acgcccagcc gtgcgatctc ggtcgcacct acagccgcgc ccgcatagat gttggcctgg
    33241 cccaagagtc ccatcccagc caggcccgcc ctcgcgcccg cgagtccgcc cagtccgccc
    33301 acggctcccg ctccttcagc gaccgccgca gtcgccgacg cggcctttgc cgccgcggtc
    33361 aagccgcgca gcgatgagat caggccgatg aatcgcacgg ccgatagcgc ggtcatcgaa
    33421 gctgtgatgg cgctgatcgc aaaagcggtc tcgggagcat gctgcttcac gaagttggcc
    33481 agtccgacca tcgacggtgt cagctcgttc tccacgtctc gcgcgagacg tccgaatgtc
    33541 tgttcgagct ccgcccaggc cttctgccgg tcttgagcgg cccggacatc gtcttccgac
    33601 agcgcggtgc tgcgagcacg gtcgagcgcc ccctcaagtt ccttgcgtcc tttcagcagc
    33661 atcgagatgg tctctgggct gaatcccatc cccaagccca tcatcttcgc gcgctttgga
    33721 tccatcccct tgaaggcgtc cgccacatca agtagcacgt cacgtgcgtc ccgcgcctct
    33781 tggctaccca gcttcgatat ttgaacgttc agagcgcgaa agaacctgaa gttgtcgccg
    33841 ccttcccccg ttgcactgaa gcgctccagc gactgggtca acttgtccat gtcgcccgcg
    33901 acggcctgcc ccgagacgcc catctgctcg gcgacctgct gccaagtcga caagccctcc
    33961 gtgctcatgt cgagcgtctt cgccatccgg cccgtcgcgg catcgaactt cgtcgagctc
    34021 tcgatgaacg acttgatcgc cgacatgccc acgaagaccg cagccatgcg caagacctcg
    34081 ttgcgcaacg tggcgaccgc gtcctggctc ttcttgagct gggcctgcag ctccttgttg
    34141 accttgctcg agcctcgccc gaggctattg tgggcgtctt ctgcttcctt ggcgcccttc
    34201 ttgtacccgc gtgggtcgag cccgagagtg accaggagcg agtcgatgat cgtcggcatt
    34261 tcagcccttc ggtgtgctca gcacgcgttc gttgtgcgcg tcgaccgcga ggatctcgag
    34321 caggtcatag aggtcgtggg tgccgagcgt cgtcgacagc tcggacatgc tggccagccg
    34381 ggagctcacc acgacgccga tcgcgcgcgg cacgttgacg tagtcggcga gctcgcgcgg
    34441 cggcccgccg cctaggccga ggtcgagggg gcggcgggca gcgaaaaacc cgtgtgcagc
    34501 agaaagactt ccttgcgcag gcgcaggcgg gtggagatct cctcgatgct gtcggggaag
    34561 aggccggtca cgaccgtccg attgtccggc tgcggacacg ccgagacgca gagcatcatc
    34621 tcgtcgagca gcggttccgc gtcggcccac ttcagcttgc cgagggcccg gatgccgacg
    34681 acggcaatgc cagccatgcc ggaggttgcg acgtcctcgg gaaggtcgac gccggcgttt
    34741 gccagcgcca ggaaggcccg gatcgcccag cgctcggcct gcgctgctgg catctcggtc
    34801 agcttgaaga ccttaccctt gtcgcgcccc tcggcgtcga tggtgacgaa tgcttcgcgg
    34861 cgagccatca gatccccgcc ggcgtgaccg attcccagac gatggtgtac gcccgcggct
    34921 gcatggtctt ggcggcgttc ggcatcatcg gcgcccgggt caggacaccc ttggtcagcg
    34981 tgatcgccgt cttcagcgaa ggcagcacga tcgagccgtt cgcgaagtag acctcgcgct
    35041 gctgcttctg ctgcgcgatc cacgtatcga agagctgatt gctgtcgctg tccgcctgga
    35101 ggtgcacgtt ccagggggtc tccatgagca cgaggcccgc cgagagcttg ccgtcgacgc
    35161 ccatcgacgt ctgggcctgc tcgatggcct cggtgtcgta gatcgcgtcc gcagcgaagc
    35221 cttggagctg cacgggggag ggcacgaggt cggcgatcga gatcgtgatg atcgagttcg
    35281 ccgcggtgag ggtcttgttt gccatgtggg ttcctcgtag aagtcgtggt ggcgcggccg
    35341 cttactgcac ggcgatcgac gacagcgaca gcttgttgac cgagtcgccg tccatgtacc
    35401 agagggtgat cggcggcgac gagcgggccg cgcgcgtggt gcccgacgcg tcgttgacct
    35461 gcagatacca gccgcgggtg gacagcactt ggtcgatctg ggtgccggcc tggttgttca
    35521 cctcggcgat ctgctgggcg gtgagcgtca cgcccgcgcg gatcgcgccg aagttcaggg
    35581 ccgcgttgat cggatccatg caggcagcgc ggatcaacgc gtagccggcc tggttgtagg
    35641 ggatgctctt gacgatggtc aggagcgtca ggagcgcgag ctgcagcgcg ttgttcagcc
    35701 agatctcgca gaccagcgag tcgagccacg accaggtgcc ggtgacgctg cccgggtaga
    35761 agtactcgaa gccctggttc gcggtcgcga cggcgccgta gaagttgtag ccgttcgcct
    35821 gcaggttcgc ggcgaccgtg tcgctggtga cgtcggccgg caggccgtcc tgcgagcggt
    35881 aggcgaagtt cgggcggccg ttcgtgcgcg tgaagtcgat cgaggcgatc gagccgcaga
    35941 tgaacgcggc cttgtcccag gtcgggccgt agatcggaca ggtggcttcg gagttggcgg
    36001 ccacgaggat cgcgcccaag ctcgcggcgg cgctggtcga ctcggtcggc gtgacgtcgt
    36061 cgtcccaggc gacgtagatg aaccgcttgt tctggctgtt gacccaggcg gcgaatgcct
    36121 gcttcaccgt gttgccgctg ccgccgtcgg ggtcgaaggc ggtcatgaag gtggcccagt
    36181 tctgggtagc gcgcttgatg ccgtccatga acgtgcccgg caccgcggcg gccgcgcccg
    36241 gcgaggtcac ggcgcccgtg gcggtcgtca gcgccaggcc ggcggccagg gatccggtcg
    36301 cgaccgagac gctcgacgag gcgccggtcg tgttgctcgt gaagacgaag gccgccgaca
    36361 cgctgtcgaa ggacaccgtg aagccaggcg acgtgaaggc cgcctggatg atggtcgcgg
    36421 cattgctgaa gctcgtcgcc gcgctgagat cgatgttgga cgaggtgtgc ggcgtgccat
    36481 cgaccgtcag cgtcaggaca ccgctcagcg cctgcagctc ggagaggatc ggcgtctgcg
    36541 acgtaccgcc gcgcatccaa ccggccaccg ccgaggcgtt gtactgcgtg aagaggatcg
    36601 agccgggctt gacgtccgag ttgtcgaagc cgttgaagta gatcgcggcg acctgcgcct
    36661 ccaccgagga atcgccgaag taggcggcga cgccgtcggc ggacgcgaac gacaggacgg
    36721 cgcccagcgg cgcgcgcgtg ctctcggtga gcactacgcc gttgaggacg agcgcgttgc
    36781 ctcccgcctt gatcacgctg ggcgtgacct cgacgaaata cgcggcgggg atggaagcgg
    36841 tcatggatgg ctccgggttg cgtgaaatgc aaagaggccg cgcgcggcgg gctctcggtg
    36901 gcggcgcggg gccgcgcggt ggaggtggaa cgtcaggccg gcgggaactc gcggtcgacg
    36961 ttgatcaggc cgacctcgag cgaggccgcg aagtcctgtc cggtcgtgat gaccgggttg
    37021 cactgcatga cgaggtcgac ctgccagcac tgctcgacct gctgctcgcc gttgaggaac
    37081 ggcgcctggc gcgggtcgga ggcgtagagc ggcgcgatcg caaggccgct gatggccagg
    37141 ccgtcgactc cggcatcgct gcgccagagc gcctctacga tcgtcgcgtt gtcgtagctg
    37201 ctggggccgt ggaagtcgag ctgcacggtg acctgctggg gaaccgtgtc ctggcgctgg
    37261 cctgcgccgg cgacgggatc cgtcagccgc ccgtcggcgt aggtcgtccg gttggtggac
    37321 agccggtgct tgaagaaggc ggtctggacg acgaagtctc ccgccatcgg ctcggggacg
    37381 cggttgctct ggccgacgat cacatcgacg cccgcgggca ggatcgccag caggaacgag
    37441 cgcagcgcgg cgagcgtcag ctcctcggtg atgtcgacgg tcgcgctcat tggccgttct
    37501 ggagtaccgc ggcgaccttg gcccagccgg cggtctggaa gaagttttcg aggacgtgca
    37561 cgacgagcca gacggtgccg tcgtccagcg tcagcatgtc gccgccgcgg ccggccgggc
    37621 gcgacacgcc acgccagtcg ccttcgacgt agatcgcgca ggcctcgccg ttgaggttga
    37681 cgccctggag ctgctgcaga tccttgtagg tcagggcctg cttttgcacg aggacggaca
    37741 ccgaatcggc gtatgtcggc gtgcggcggc cggacggcgc agacgcgctg cccgtcgacg
    37801 cgctgtactg cgcctgcacc ttcgggttca cggcgccgat gacgccgccg acgagcgcgc
    37861 tgaggttcat ggggtcactc cgcctcgccg accaggtgcg tcagcgagga gatcatcagg
    37921 cccgtgtcgt gcagcgggtc ggtgcgggtg ctgccggcct caccgcgcgc cacgcgggcc
    37981 gcggcttcgg cgaccgcgcg gcggttcatg accaggctcg ggtcgtcgtc gcgcatcttg
    38041 cgcagcatca gcgtcacagg cgacagcggc gcgaagctcg ccgtgcccag cgtgtgggcg
    38101 atgtcgccgg cagcctgcag gccgacgccg tccagtacct gttcggcgct cgcgcggccc
    38161 ttgacgacgg cgcgcatgcc gtcggccatc accttgaccc aggcgccctt ccgatcggcg
    38221 atggtcggcc ggatgaacgg acgcgcttgg atgccgcccg cggagtagcc gttctcctgg
    38281 atcgcggcga cgtacgcgac gggcgtgccg tccttgtact gcgccgcggg gaaccagccg
    38341 accttcgcga cccgttcagc gaacccctcg gggatcgcct ccaggcgcgc gcggatcttg
    38401 tcgaagttga ggcccttcac cagacgcccc cggccttcct gtacgacgag cgctcgggcg
    38461 agccgccgat caggaagcct cccgcggcgc gcatgctcag gagcgcccac aactgcaggc
    38521 cgtacggcgt gctcgcgagc cagaactgcc agccgctggt cgtgggcggc ggctgcatct
    38581 ggacgctgac gctgccctgc gtcgcgccgt tgatcggcgc cacggacgtc tggccagcct
    38641 gcagcatcgc cgccgactgc gccaggtggg ccgtgagcag gctcagggcc cggtccatca
    38701 ccgcaccgcg caggatgcag gtgtcgttct cgccgaggaa ggccgtcgcg tcctggaagt
    38761 agacggcgag ctggtcggcc ggcacctgcg ccagggccgg gaacgacttg cagaacgcgt
    38821 cctggtcgaa ggtgtgagcg gccatgggct gcccttacat gttcttcgcg gcgggcttgg
    38881 cgtcgtcgtc gccggcgttg gcgtagtcgg cgggcgtctt cggcgccgag ggatcggcgc
    38941 ggttcatgtc ggcggcgacc ttctccgcgt cgacgtcctt gcgctccacg cgcatgtggc
    39001 cgttcttgac gtgcagcttg aacacctcgt tcttctggag aacctcgagg tcgctgtcgt
    39061 cgatctcggt gcggacgccg ctcggggtca cgatgcggtc gttggcgacg cccgagccgc
    39121 ccctgatgaa gacgacgcgc tcgacgttcg cgatggcgcc cttctcgaca ggcggcagcc
    39181 aggccgtgta gtgcatgtcg gaggtgagcg tggaatagat gtagaccttg gccatgtagc
    39241 ctctccaaat gggaaggccc cgcggggctg ccgcggggcc ttcagttgtg acgcggcggt
    39301 gtgccgcgga cagttcagcg atgcgtcaga tgcccgagcc gcgcacgatc gcgtaggggc
    39361 gcttgaccat gacgccggcc gtggcgttcg agtagtcctc gacgtaggcc ttcgagcgct
    39421 tctcgacgcc cagcaccatg aacttcgccg gcacgatctg ctcgatgacg cgcgagttgt
    39481 ccgaggcgcc gtcgtccacc gactccgcgt acaggtagaa cacgttcgcg ccgccgttgg
    39541 cgccgttgag ctgcggcgcc gactcgatgc gcatcttcgg gtagttctcc gccacccact
    39601 tgcgcaccga catgccgaag tcggtcgccg tggtcacggc ctgcgccacc gcggtcggca
    39661 gcgccagggt cgtggcatcg gtcagcgggt tgacggtgtc ctgcgactgg gtctgcagct
    39721 tcgtcgccgc ctggcggatg tcggcggtga tttcgaggaa cgtcttgctc gcccaggtcg
    39781 gcgacccgcc ggtaccgttg ggcaccgtgt agtacgccgg caggctcggg tcgttcagga
    39841 agccgtaggt gcggttgttg cccgagttgt agccgaagaa cccgaccagg ttccgctgga
    39901 tctcgagggc ggtcgccgag ctgttgcgct tctcggccgc cgtgttgagg cggatccgcg
    39961 cggcgcggcg ctcttcgagg acgccgacct gcatgccctt ctcgaatcgc acgacggtgc
    40021 ggcgctcgaa gttcgtgttc cagctggaga gcggcacgtt cgtcaggtcg ccgtacggga
    40081 cggcggtgcc gacgggctcg agcacgccct ggacgacttc ttcgtcttcc cagtcaccga
    40141 tcgtcgtcat gccgatcagc gagtcgatct tgcgcgccgc cgtgatggtg cgcacgaagc
    40201 ccggcagcca cgtctgcagg aactggaccg gcgtggtgat ggaggccgcg gtcagcagac
    40261 cctgggcgtc gtccatcgcg aattcgtcga gggcgatctc ggccatgcgg tcgatcttcc
    40321 cgcggggcag gccgatgcct acagcagaca gcgccaggta gttggcgacg tccgacgcct
    40381 tcatctccag ccggcgcggc atgccgtcag gcagttggaa gctgcgcggc gagagatagc
    40441 cgtgctcttg ggacttcatg gtgttgatcc tccgatcagt tggtcaggcg gatcgacacg
    40501 aggccggccg cggcgttgac gacttcctcg acgaagcagt tcggcacgta ggcgttgccc
    40561 gcgccggccg cagcgccggg agcgaccgcg gcgagggcgc cggtggtcgt gttgtagatg
    40621 acttggtcac ccatgttggc ggccgtcgtc agcgcgacga ccaggccatt gcccatcagg
    40681 acgaagtcgc cttcgctgtt gtcgggcagc gtgagcgtcg gcgacagcgg ccccgacgag
    40741 ccgccggagc tcgagtactc cttcggattg gccaggatgc cggcgaagat cgacgtgccg
    40801 ttggtgatgg caccgccgac ggacgcgacg cccgtgccgt tggccgccgt gtaggcgtag
    40861 ccgatgacgt tcggattggc gccacccgag ttgatcgggc gacgcacgac acggcgcggg
    40921 ccgtccttgt tgaactcgcc cggaacgccg aaggcgtagg tgatgttgac ggtggactgg
    40981 aaagcggtca tcgcttactc cccctgcagg aatcgggtga cgaagtcgcc gccgccggag
    41041 ctggagtcca tggcgacggc ggtggtggcc ttcttggccg acggcgcgac cgcggcggcg
    41101 aggtagccgc tcagcaccgc cgcctcctgg ccggcggccg ccttgatgcc cagcttcttg
    41161 acgccgtacg cggcgacttc ggcctcggtc atctcggagt ggtcgaaggt gccgatgtgg
    41221 gcggacagct ctcgggcgag cttgtcgcgg cgcacgacct gacccatgac cgagcggaag
    41281 acggcggcct cgtccatcgc ggcgctgccc ttgtcgtcgt cgtccttcgg gggcggcgtg
    41341 ccggagtcgt cgtccttggc ggcgacggtc ttggcctcgt ccttcttctc ctcctcggtc
    41401 ttgcccggat cggcgtcgac cgcggcggcc gtggtggtgg ccgcgcccgg attcgcatgc
    41461 tccatgagca tcttgatcgc gggcatgacc tgcttgaacg cgtcgagcgc agcttccagc
    41521 gtcatgccgg agccgccctc gtccttcttc tcgtcagcca tggatggctc cttttcagag
    41581 gtggtgggtt gatcgatcga gtccaggacg gcgacgtccg ggcccatgcg gccgctcttg
    41641 acgagcgcca ggtggttgcc gcgaatccgc cgctggattg cgtcgtacgg ctggcccttg
    41701 aacacgccgg gcgtccagtc gtacttgcag cggtagccgc aggacaactc ccgcttcccg
    41761 gcctcgatca gggtggccat ggattgggag aagagcttca cgttcccgcg caggacgccg
    41821 tcgtccgtgt ccgggtcgaa atagacgtcc tcgccgacga cgccctgcac gcccttgcgc
    41881 tcggccggca tcaagccggc ctcttcgggg cccagcatca cgtggttgtc gatccacggc
    41941 agcaggcgga agctcgcgat ggcttcgggg tcggagagct cctcggcggg tcgcagaatc
    42001 tggaacaggc gatcgggctc gtcgggcttg ccgacctgcc ggccgcggta ggggaagacg
    42061 ccgaccttcg acagcgggtt gcccttgatc tcgaaccagc cgttcgtgtc gaagtcgcgc
    42121 gcggactcgt ccatggcgac gtcgtcgggc tcggctggct cctcgggcgc cggcgccgcg
    42181 gcggccagcg ccagggcgat ctggtcggcc accatcggat gcagcggcgt cggcagatcg
    42241 tccgggctcg cccagcagta gccgaggtgc tcgtcgttca gcaccggtgc gaaggcaggc
    42301 tcggtgcaca ggaacagcgc gaagccgtcg cgctcctcga gcggcgcgac gacgtccgga
    42361 cgatgtcccg tctcttccat cgactcgcgt gcggccgcct ccaggctgcc ctcgccctcc
    42421 tcgatgccgc cggcggggaa gccccaggtg agcgggtgcg catcggtcga agcggagcgc
    42481 ttgagcagca gcacacggcc gtcggccacg taggcgatgc cggcggcggt gggcgtgatc
    42541 ttcgattctt cggtcatggc gtcgccaatg caaaaggccc gcgcgcggcg ggccttgctg
    42601 gtggtgtcgt cggagaggtc agtcggcgtg gaagcggacg atcggccgca tcttgcagcg
    42661 gcagccgatg gcgtcacccg gcttgccgcg ctgacccgtg cgcttgtcga tgacgggcgg
    42721 atccttgagg ctgtagacgc tcccgctcat ctcgatgtgg tcgatgcgcg gctccttcga
    42781 gccagcggtg tgcacccatt cgtactcggg gcagtcgatg tccagcatcc gctgggccgt
    42841 gatgccgctg tacgccttgc gcgtctgatc catcgcgacc aggcgcgcgt ggcggacgtt
    42901 ctgcctgtag gccttctcca ggaacggcgt cagatccttg aggccctgcc cggtggtgat
    42961 ggaccgcatc acggctccct gcaccttcgt gaggtaggtc gtcggaatga ccttgatcag
    43021 gcccaccgcc tcggtcgtgc tcgcgttgat gatgtccagc agcgccggcg acatcgcctt
    43081 ggcgttgagc tgcacggact ggctgatctc ccgcagcgac atgcccagtg tcaccgcgct
    43141 gttctggatc gtgcgatcca tcatgcgctt cgtcgccttc ttggcgagcc ggttgaacat
    43201 cggctcgtac ttgtccatca gcgcgttgag ctggatccgc gcttggctgc tgatgttcga
    43261 gaagccggcg atcgcgccct cgcgcggcgc ggcgtcggcc gcgtcatggg cggcgtcggc
    43321 gaagacccgg gcgaccgtct tcttcgtctc gtcggccagc cgccggatca tggccaggat
    43381 cgccacctcg aagtcgcgct ggatcgccgc gttggggctc agcacgcccc cgcgcagcgg
    43441 ctcattcggc cgcgggatcg tcgccatcgt cgtcctcgag gtcgtcgtcc ggcgccggag
    43501 cggtcaggtc gacacccgcg tacccgctcg ccggatcaag cgcgatgcgc tggcgctctt
    43561 cctcgctgcc gatcgcgccg gagctgacca gcgcctggcc ggtctgcgcc ttcttgaggt
    43621 tgagctcggc ctgttcgccg gccgtcgggc tgtcgaccgg acgccactcg accgtcgtct
    43681 cgatgtcttt catcgcggcg agcttcggca cgacctcgga gcgcatgacc agcaggtggt
    43741 ggcgctcgac cagcggcgtc aggtcgtggg cctggatgct ctccagctcc tcgtggtagc
    43801 tggcctcctc gaactcgccc gtggcgttga agcccttcgg cgaggtgccg agcagcttcg
    43861 tcgcgggcac gttcgcggcc gcggcgacga tctggtactg cgtcatgatg acgctgtcca
    43921 ggtcggcgag cgacgtgtcg aactgggtga actcgtcgcc ttccttgtcc ccgagcttga
    43981 tcgcgtagtt gtcgcggaag cgggcccacg tgctcatgcg ctcgacgcct tcgtcgccca
    44041 gcgccgcgaa ctgcgacatg tccgtgcgcc acaccgtggt gcgtttcgtc tgcgcgagtt
    44101 gcggcgcctc gttggcggtg cgctcggcgc cgtagacgcg ctccatgatc cgctggggca
    44161 ccggcacacc gccgtacagg tactgcggct tcagcaggtc gggcacctcg ctgttgcgga
    44221 agatcacgag gtgcgagcgg tggtagcgct ggccgttgat ctgccaccag gtgggctcgt
    44281 agaagtgcgc cgtgtccggc cgcgaggacg ccgcctgatc gagcatcggc gccgtccagt
    44341 acgggtcgac ctggacgatg cccttgtacg agtcgggcgt gacgccgtcc gggttgaacg
    44401 gcttctcgta gtactcggtg tcggtgctct cgaccttgaa cagcgcgatg cggatgccga
    44461 agatccgacc cttgcgcacg aactcctcga gctgggcgtt cagcttcacc cggcggtcgg
    44521 ccgccttcag gatcttgatc gcctcgggcg ggatctcgtc gccggacgcg ctcacgatgt
    44581 cgaagccctg gcggatcgcg tcgcgcccgg gcatgccgca ggccttgtcg atgagccagt
    44641 gctgcgccag gatcgcggcg agctgatggc cgatgaacgt ctgcgacgcg taccaggcgg
    44701 ccagcacttc cggaatgttc tgcgcggtcg cggccagctt gagcgcgatc ccgtcgccgt
    44761 tgccgtcatc gtccatggcg ccctcgggcc ggaacttcgg caagccgcgt tgcagagcgt
    44821 caagataatc cgccacgcga agcgcgccgc cgcggcgctc tgccgcgtga gtgctccaga
    44881 acgagccggg ctcgcgcgcg ggcgccggcg gcgcggcaac aggctttcgg cgcaaccagg
    44941 agaacatagg ctatccaaag aaggagcggc gcacgacgat cagctcgccg aatgcgcgcg
    45001 agagcgcgtc gacctggtcg tcgaacgatc cgttcgggaa gacgcgcagc tcggagatga
    45061 aggcggcatt ccagtcgccg cgcagcatcg acacattgcc gacgttcacc tgcgccgcgg
    45121 ccggctcggc ccgggtgacc ttgtcgccag tctcgggcga cgtcgagacc ttgtagcccg
    45181 cgagctggcg cgtcaggtac agcacctgcg tcttgcctgc ctgccccggg tcttgcggga
    45241 tgctctgctt cgtggggcgc ccgtcgcgcg ccgccgtgtt cacgatcgcg gcgtcgcgct
    45301 ggtcgggccc gcagcgcatg cggttgacgt ccgcgatgat gaagcggccg tcctcggtgc
    45361 gaccgagccg caggccgacc gtccagtcgc cgtccgtcgt gctcgccaag tcccagccgc
    45421 gcacccagtc gatccgcccg gccggcagcg cgtcgatgac gctgatctgg tcgggcttga
    45481 acaggccacc atccagcggc gcgggccgct gccggtactg gccggcgaac acgtagggtg
    45541 acgcgctctc catgcggcgc agcgcctcgg catcgtgctt ctccggccac agcggcgtgc
    45601 cgtcgtcgtt ccaggccgag aggcagaggt gctcccacac ctcgccgttg ccgcccggcg
    45661 caatgccggg ctgctcgccc agcagccagc ccgccaggtc gtcctggtgc agccgctgca
    45721 tgatcacgat gatcggcgtg tccgggctgt tcttccggct ctcgagcgtc gtctggaacc
    45781 agtcgatcac gccctggcga atgacgtcgc tgcgcgcctc gtcagccttg tgcgggtcgt
    45841 cgatgacgat cgccccgccg aagccctccc ggtgcttgcc cgcgccgaag ccggtgatcg
    45901 tgccgccggt gcccgtcgcg tacatgactc cgccggccgt cgtcttccag tggtgcgccg
    45961 cctcaccgct cagcgccacg cccggaaaga tttcccggta ggcctcgtgc tgcaccatgc
    46021 tgcggatgcc cgcgctgttg ttgaccgcga gctggctgct gtagctggtg tggatgaact
    46081 cggcgtccgg cacctgcccg aagcaccacg caatccagtt gatcaccgca agctcagtct
    46141 tcgagtaccg cggcgggatg ttgatcacca gacgcttgca ctcgccgcgg tagacacgca
    46201 tcagcgcgtc gcagatcagc ttgtggtgcc gggcccgctg ccactggaag cccttgcgct
    46261 gctggaacat ccagcggctg aagaagtagt ggtcggcgcg cgcgagctca gcggccgcga
    46321 accgctcctg cggcgagaag acccgcacgc atcacacctc gtcggccacc cgccgcgcga
    46381 tctgctggaa ctcctcgagc gtcacggacg tggtggtgct ggcctgctcg atcgggccgc
    46441 cccccttgcc cgtgatctcg cggcggttcg tgaacgcccc gccgacctcc tttgccgcct
    46501 gctccacgag ctgcgcggcg agagcgacgt tgcgctggtt ctcggcgagc gtggccagcc
    46561 gctgaagcgt gcgcagacgc accgcctgat tggcgatcgg gatctgcgac gtctctttca
    46621 ggaacgccgc cctcgtcgac tcgaacagcg cgacgagctt ggcgctgatc tgcccgcggc
    46681 cggacacctt cgacgggtcg tactcggcca cctggcgccg gtggatctcg atgccgaact
    46741 cctcccgaac cgcgtcgacg atctgcgaag gcgtgtcgaa gcaagcgagc gcctgaacga
    46801 tgaagagctt ctgcggctcg gtgagctttg ccatggttgt cggggccggg ttcgggctaa
    46861 gctgccctta ggagacacgt gccgcacgca cccgcgacct gcaggcggct gacttcggga
    46921 gcgcgccccg ccgcctcgac caggcgtgcc aggttgccct ccgggttgcc ggttccgtac
    46981 cggcggacga cgcccacgaa ctcttcaacg tcgtgccctc ggatctctag gcgcgggcgc
    47041 ccttccttcg tgaacagcgg ctcgccgaac aggccccgcg cgtgcgccac gtggtagagc
    47101 tcgtgctcta ccagcgcgca ccactcagcg tcggtgcaat cggcggcgta gctcgccgcg
    47161 aaggtgatca ggaacttggg tgcgacgccg aaccagtcgg cgtattgctg ctcggcgcgc
    47221 atgcgctcga agccgccgga gcggaagctg acttcctcgg cggttccgac tacggtgcgg
    47281 cccttcttcg cgaatccttg cggcgcccac atgacctgca ggtcggcgcc gatcaggtgg
    47341 cggtgctccg ggttgtggag ctcgcctgtc tcccgcagga tgtagcgctc gatccactcg
    47401 agcacttcgg ccgcgggttg catccgcccg tccgccggcg gccgcgggcg ccggggcacg
    47461 cgcggcgcgc tggtgcgggc cacggtcagt cctcgcagat ccgcggcgcc tcgatgcgga
    47521 tcggccggcg cagctttccc gtgatcttca gcgcccaccc cgggttctcg cggcccgcgg
    47581 cggccagcac cgtgaagcac aggccgaaca tcgcctccga cagcaggaac agcgggatca
    47641 gcagcggcga gcgcgggtcg atcagcgggg cctcggtgtc caggtcgccc aggtagaccg
    47701 ggcagacgcc gaaccagccc ttgtgcgtca gcaggacgcc ttcgacgggc gcgcgcggtg
    47761 tcattgcctt actgcgcggc ggtggtcgcc ggggccgtgg gttgcgcgtc gggcgcgggc
    47821 gcggcggccg gcttgtgccc gaagatgtgc tcgccgaagg cgcggaagaa cgaggccacc
    47881 gccgccgcgt cgtgctccag cacgttggca aggctcggct cgacggactt cagcttcgcc
    47941 tcgatggcgt cggcttgcgt ccgcaggcgg tcgacctcgg cttgcatcgc ctcgcgaatg
    48001 gtgggcatgt cgttctcctg ttgagttgag ggtgcaggcg cccgccgcca ggcgccggag
    48061 gaggccggcc ggcacgttca ctcgcgtcac ctgctgccgg cgatctttcg cactctggcc
    48121 ggcttgtgcg ggccgctgcg ctccgatgtg gcccatgacc aagcaggtcg gcgggagtgg
    48181 cccgggtggc gcttgacgcg gcgcttggcc gctcccgcgg caaggatcga ggggccggtg
    48241 ctgatctccg gcttgccccg ttcagcgtct acggggcgcg attgaatttc cgactctgga
    48301 cttcactgcg catcagcctg cgcattccct cgcgaaagcg atccccggct cacgctcccc
    48361 ttcggcctcg cggcctccgc tgctgcgcga tcgctttcgc gaggagccgg cttccgccgg
    48421 gtctcgcggc ccgtcttccc gggctgtcgt ctcgcctgac tgtctcgccc cttgccgtca
    48481 ctggctgacg gggttccaac aacagtggca gcgcgggttg cctgccgatg tgcgtgaatc
    48541 cacgcgagcc cttttgcggc cctggcaggt gcttcgcgcc ggctgaccgg cgaaatggga
    48601 atgatcagtc gggggattcc ccgtcgcggt ggacgagctc gagctggtcg cactggccgt
    48661 agtcgacctt cacgcccacg agcgtggcgg tgatcgggcc atggccgatg cgctcgcggt
    48721 gcacctggaa gtgcccgtcg ctcaggcaca cggcgtgggc gtacggctgc tccaccttgc
    48781 cgatgtcgcc ggcatcgcgg taccgcacga tgccgacgcg gcagttgcac gtgggcgcgc
    48841 cgggcgtgat acccagcatg acgccgacga ccacgccggg cgtgttgtag accttgcctt
    48901 tgaccaggtc gccgaccttg gcctcgcgac cgtccttgta gtgcatgcgc gtctcctcgc
    48961 gatgatggtt gcgggggccg ggctcgaacc ggcgacctcc gggttatgag cccggcgcgc
    49021 tacccactgc gctaccccac gtcttgatcg ttcagccgct cagatcgatg ggcacatgct
    49081 ccagcgagcc tcgatcctcg gcgcgtcgcg cttcaccatg cgcttgacga aggcgctggc
    49141 cgcggtcaga gcggccgacg agcgcatgtc gacccggccg ccggcggcgg gctcgacgcg
    49201 gaacgccgaa cgcacgacgc tcactgcgaa cgcgaaggca tcgcgcgcgg ccgccacgac
    49261 gcgcgcgagg ccgtcgaacg ccgtggaggc gacgctcatc acgacggcga gcgccaggct
    49321 cggaaggact cggagggatc ggatcatcgg aggctcactg gttgtcgccg gcggctcccg
    49381 gcgggaaggc cctggccgta gcgggcaaga tgtcaggccg tgcggtcggc gaggctcgcg
    49441 ctgacgttca cgcccgcgcc gatcacgccg gcaccgctgg tcgtcacgtt gccgtacatg
    49501 ccgacgtgga tgtcgcgcgt gtcgtcgtcc acgagggcgt cgatgaaggc gcaggcggcg
    49561 gcgtacgcct tgtcctgatc gaccgcgtgc gtcgactggt ggcgcaccac ctccgcgagc
    49621 ttggcggcga cggagttctt ggcctcggcc ttgttggcgg cgcggatggt gaaggaatag
    49681 gacatggatg ggctcgcaaa gaagaagccc ccggggcgtt tccacctcgg gggcaagcac
    49741 gtcgccttct cagactcttg gtgtgcccgg cgcggacgca cctgtccatc gacagggccg
    49801 tctgcggaac gggcgccagg ggcaaccccc tggggacaaa ttcaacgcgt gggccgaagc
    49861 tgacgcgccg ccgtctgcag cgcccggaca gggctggcat gcagcacgac gtgattgtgc
    49921 acaggtcgca taagagcgat atcgagcttt gagtcagccg accgcgccgc tgcccgcggc
    49981 ttcagcccgc agcagcatcg ccttggcctc gcgcaccgca cgacgttgcc gcgcgagact
    50041 cgccgccgcc gtgtcgacga gatccgagag gtcgagcagc agcgcgaata cgaaggcgcg
    50101 acctatctca tccttgccca cgctgtacct ccggctgccc gtggccttgc aggcgcggca
    50161 ccgcttctgc ggctccccgc gctgcagcgc acccgtgaac ccgatgccgc ggcattcgcg
    50221 gcattgcgga gataggaaga cgtccagcgc cgcgccagca aggcgcagga tccgctcggt
    50281 cgggaggacg aacctcagtt cccgggcgcg tgccagcgcg aagtgaccca acatttcctt
    50341 ggtgcgccgc aggctgggca gcgtcgcgat gatcatgacg tggtcggtca aggcctgcgc
    50401 ctgggcggcg ctgttgattg cgctcgcccg ctgctccgct gtggacagga cgacaccgtc
    50461 ctcgtcgacg aagtccccct tctgccgcgc ggcgtcgatc tccgcggcgc gcagccgcgc
    50521 ctctgccgcc cggtgctctc cacgcaccgt gtcgtactcg acctgcaggc gcagcagcgc
    50581 gccggcgagg cagtttcccg ccagccccgc cgcgaccagc aggtcgacgt cgcccgctgt
    50641 ctcccgcacg cgcagattgc tcgactcgat cgccccgctg tagcgctcgg ccatcgtcac
    50701 gggctcgtcg ttcgtcgtcc tcatgccttc gttcccttct tctgcgcgcg cttggcgcga
    50761 tcaattgcgt tcgccgtggg cgccgggccg tcgcacggca cgcccatggc cttcaagacc
    50821 ttgaatccgt cgtactcggc gcggtcgatc gtcatcacga acgccgcggc gtcgccgatg
    50881 gcgattccca ccaggacgac ctcgctgtag ctgtccgagt cgctgatggc ggtgatccgg
    50941 tcgccgcgtg cgtgggcggc tgcgacgcgc gcttgcagcg cctgctcagg cgtgggcacc
    51001 agttcgggct tcacaggacg cccttctcga gcaggcggcc caggctgcgg aagtggccca
    51061 tgcaccacgc caggtcgatc tcgtcgcggg tcaggtgcgg cgcggcgatc tggccgtcgt
    51121 agaccccatc gcaggcggtg caggcgatcg caccgcagac gtcgagcgcc ttcgtcgcca
    51181 tgccgcggcc cgcgtcgccc aggcgcgcgc cccagcgggc gtgcgaccag atcgagaact
    51241 cgggcgcgaa ccggcagacg cccggcaggc gcaccgtgca gggctcgccc ctggctgcct
    51301 cgcggatgcg cgcgccccag gcctcgacgg cgcccgcgac cgggcggcg
//
LOCUS       DASVLN010000278        20874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_499291, whole genome shotgun sequence.
ACCESSION   DASVLN010000278 DASVLN010000000
VERSION     DASVLN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 20874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20874
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499291"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VF453_06690"
     CDS             <1..328
                     /locus_tag="VF453_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687372.1"
                     /translation="AAATSPAPVAPLPTAELAPEHPPITAAAPAPVEPAPTAAPASPV
                     VATERATLTLTQINERLNGPKLDAACLAKLGVPQVESKGPAVRIDAEDWALLKAALVA
                     HIEALA"
     gene            325..822
                     /locus_tag="VF453_06695"
     CDS             325..822
                     /locus_tag="VF453_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687373.1"
                     /translation="MNRRHRARRAVVARPTFSLLDEAMASNTEPLARARRVEQLTRMW
                     EGFDAMSTASMPTTDDWRVCSDAVNLMESLVDLGYVEDGSKLLEDAVAALARAGQRAT
                     DGKALRLDGPGRDAVRAVLLDYGSALELLPARAFIQCHRRTERRIREILAGRGQAHDI
                     EVVSL"
     gene            840..1070
                     /locus_tag="VF453_06700"
     CDS             840..1070
                     /locus_tag="VF453_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687374.1"
                     /translation="MTTTTPRRRRVKKQPDARPGVDMLASQPPTAPAPVRLPKPRGPA
                     NMPGEPIVTERTKITRCPSPSLGLHTNTHAEA"
     gene            1090..1290
                     /locus_tag="VF453_06705"
     CDS             1090..1290
                     /locus_tag="VF453_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687375.1"
                     /translation="MRYSQDKDIAKSVAELLREGWTYTRGNRHPRVVAPNGKFVSFSV
                     TPSCPHAAARFQRDVQTIKATL"
     gene            1287..1994
                     /locus_tag="VF453_06710"
     CDS             1287..1994
                     /locus_tag="VF453_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687376.1"
                     /translation="MIVIEQPKVEEVRDAAGDIERRLTHPAFALIRATRMTGHCALYG
                     TDFIHNNLVKIAIGRSALHRSTSHDWPAPGAELVEVAMSEAQWARLISSTGGVDAETP
                     CTLVKLGTDTVPGLPPPESRADEFADELRNTQSRAISSLGDLRAAIDGLGLSKRSAAS
                     LAAMVDNALTQITENSEHVVRQFGKHAEHLVEEVKTEVSAWMQTMIASAQAAARLGDR
                     SAPPLPPLELNGNGNGK"
     gene            1991..2215
                     /locus_tag="VF453_06715"
     CDS             1991..2215
                     /locus_tag="VF453_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687377.1"
                     /translation="MTAAAESSCRACGHAPIDGCSRVECPERKAWSAGALDDLAPAAH
                     SGVFSTNGQDGHVQVGTAGCFRRVKHRGAE"
     gene            2230..2829
                     /locus_tag="VF453_06720"
     CDS             2230..2829
                     /locus_tag="VF453_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687378.1"
                     /translation="MSINIEKFAGVDDFRTYINRPWRRDSKLYATNGHFMIEIDDDGR
                     DAAPFEKQPDCVGLFAKYTPGDFMPIPPLAAPVHCQWCKGRGACYRKACPDCDGQGEF
                     AHGMYDYDCKRCEGDGYFLFPTAPPGEKETRCSACYGLGEEVRKDQGTPVGELLVATR
                     LLRVLTELPGIEVAVCKAPEALWFRFTGGRGLVMPMRKG"
     gene            2833..3303
                     /locus_tag="VF453_06725"
     CDS             2833..3303
                     /locus_tag="VF453_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687379.1"
                     /translation="MSTPPKDFGPLSNGDLTRIAESVGMVRSFDGVLMRSIGHAGGMT
                     AEHGFAFARAAISEDRARQAAAAPKPAGALDENIALREMAKHAHRALNAAAAILATID
                     EEELGDQRVADVEKRCTQIACSLFSLLRGNEVDRYESSPLETAALPLAGTRGAA"
     gene            3300..3767
                     /locus_tag="VF453_06730"
     CDS             3300..3767
                     /locus_tag="VF453_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687380.1"
                     /translation="MKLADRFIARVTRRPPDFVIGGAARPYLRRWWVIPRNRLFNVYL
                     HQFLRSDDDRALHDHPWANASILLRGRYVEHTISAGGIEHRRELRAGDFRLRPSGRFA
                     HRVELVDGPCWTLFLTGPRYREWGFHCPRAGWIHWEKFTAADDVGAVGRGCDQ"
     gene            3842..4045
                     /locus_tag="VF453_06735"
     CDS             3842..4045
                     /locus_tag="VF453_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEX7687381.1"
                     /translation="MVVPLEWIKLDRYCELTGDTPGAVHARRRKRQWSNGVECIVGPD
                     GNLWINPAAFNAWVRREKAPETT"
     gene            4042..5235
                     /locus_tag="VF453_06740"
     CDS             4042..5235
                     /locus_tag="VF453_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HEX7687382.1"
                     /translation="MTAPKKLKEITLPRGVKIREFATERRLQVAFSYRGEECRELLPP
                     QPITQTTANLAGGLRQEIQRKIVEGSFHYADYFPGSARAARFSAGTSRALITDKLEAQ
                     REIYRRQVKEGRMSPSTLHGYEKAIDSAKYRYWEGKTLADATPAALREWIGGFGVTAK
                     FARNLLIPMRSLMEDALNDDLIDEDPFNRIALNKLLRQTAADSDYHVDPYSADERAAL
                     LAAARADERPLVAFWFETGLRPGELFALRKTRILRERTVARVDTNWVQNTEKAPKTAA
                     GNRAVDLSSAALGAVEAQLEITPQAIEHLFINPTTGRPWENDTQLRKSLWIPLNERAK
                     VRYRNPYQARHTFASTRLTAGHNPWYVAQQLGHANVQMVFQVYGKFISDDYQPPKAAA
                     KGDGR"
     gene            complement(5405..6265)
                     /locus_tag="VF453_06745"
     CDS             complement(5405..6265)
                     /locus_tag="VF453_06745"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEX7687383.1"
                     /translation="MRLLRYGTRGNERPGLLDAQGRVRDLGAVVPDLRGDVLTPAGLD
                     RLRALDPSSLPLVSGRPQDDLRLGPCVAGVGKFICIGLNYADHAAESGMPIPTEPVVF
                     GKWTSAITGPDDDVEIPPGSTKTDWEVELGVVIGEGGRAIDERDAMRHVAGYCVVNDV
                     SEREYQLERGGQWDKGKGCDTFGPIGPWLVTADEIPDPQALDLWLEVNGRRYQSGNTR
                     TMVFGVAHIVSYLSRFMSLQPGDIISTGTPPGVGLGQNPPTYLAPGDVVRLGIAGLGE
                     QRQRCRMAGR"
     gene            complement(6282..7019)
                     /locus_tag="VF453_06750"
     CDS             complement(6282..7019)
                     /locus_tag="VF453_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7687384.1"
                     /translation="MSQRLQGRTAFVTAAGNGIGRAAALAFAAEGATVWATDIAPESL
                     ATLGHGLIRTRVLDVRDTAAVNAAVAGAGRIDVLFNCAGIVHHGSLLECDDDAWARSM
                     DLNVASMHRVTRAALPGMLAAGGGSVINMSSMASSIKGFANRCVYGASKAAVIGLTKS
                     IAADFVRQGIRCNAVCPGTVDTPSLRGRIAAAADPAQAERDFIARQPMGRLAEVDDIT
                     PLLVYLASDESRFVTGQALSVDGGVTI"
     gene            complement(7055..7765)
                     /locus_tag="VF453_06755"
     CDS             complement(7055..7765)
                     /locus_tag="VF453_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX7687385.1"
                     /translation="MPPVRQSDTRRLYQQVADQIRAVIADGAYAVGSRLPPERELALQ
                     LGVSRPSLREALIALEIDGRVEIRMGSGVYVCAPARRADHTPALGESPSELMHARAAV
                     ESAVIVLAAARVTRPGMERIRGILDAMRDDHARGRGPLENDRRFHVAIAEMGGNSVLV
                     RLVGELFDERHGPIASRLSVRSENVATWSEALAEHERIYEALDARDPQAAAAAMGMHL
                     RASQARWVGGTATDEPGK"
     gene            8029..9591
                     /locus_tag="VF453_06760"
     CDS             8029..9591
                     /locus_tag="VF453_06760"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX7687386.1"
                     /translation="MSDAASAPLLELDGVCKRFLGVVALEGVSFDLRPGEVHAVCGEN
                     GAGKSTLMKVISGEYQADGGTIRYRGREVRFPSPLAAQRQGIVMIHQELNLVPHLSVA
                     ENIYLAREPRRGWFVDRARLRADAQANLDRLGGGISPDATVLSLPVAQQQMVEIAKAL
                     SMRASVLIMDEPTSSLTETETTRLFKVIHELKRAGVAIVYISHRLEEMAEIVDRVTVL
                     RDGRYISTMPFAGTSVDDIVARMVGRALDDKFPPPTRQPADEVLLSVQELRREGRFGP
                     VSFELRRGEILGFAGLIGAGRTEVARAVFGADPVDGGSVRLNGRALAIRSPIDAIRHG
                     IAYLSEDRKAHGLAVKMTLAHNVTLANMHAVSGRRGFIRFAQEIAVARRYIDALGIRT
                     PGPRQVARLLSGGNQQKVVIAKWLFRQSQVIFFDEPTRGIDVGAKYAIYELMDRLAAQ
                     GVGVVLISSELPEILGLTDRVAVFHEGRLASVLETGRTTQEEIMRHASGSGAAGRAST
                     VPPDLDLNLVAP"
     gene            9588..10571
                     /locus_tag="VF453_06765"
     CDS             9588..10571
                     /locus_tag="VF453_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7687387.1"
                     /translation="MNDRQKDLVQKFAALAGLLVLATVFSLTSRAFLTVDNGMTVALQ
                     VTSIAYLGIGATFVIITGGIDLSVGSVLALSGIVAALCVKSGMPVALGMSAGVLAGTL
                     CGAINGLLVTRLKLPPFIATLGMMLVARGVALQVTGARAISGLGDAFGELGNGSLGRV
                     VRVDASGFPDIVFPGIPYPVLMMVVLAVVASVVLTRTTLGRHVYAVGSNAEAARLSGV
                     NVSGVVLFTYVVSGALAGLAGCVLMSRLVTGQPNEGVMYELDAIAASVIGGTSLIGGV
                     GTISGTAIGSFVIGILRNGLNMNGVSAFTQQIVIGLVILLTVWVDQMRNRR"
     gene            10642..11574
                     /locus_tag="VF453_06770"
     CDS             10642..11574
                     /locus_tag="VF453_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7687388.1"
                     /translation="MLPRLVLLTSLAMAAATACAGEIAVIVKTTNSTYWQNVNKGASA
                     AIKEIPGRTMTFSGPASESAIADEVNLVENAVNRHVDAIVLAASDPDALVPAIKKAWE
                     ARIPVVLIDSMVSDAGKPYYQSFLSTDNKLAGELCAKQLIADVGTTGKIAIMSYVAGA
                     GSEVGRVGGFKDYIAAHSKLQIVGPFYSQSQMATALNQTTDVLASNPDLKGIFGANEP
                     TAVGMGRALVQSGKAGKVVAVGFDGNEDLQKFVRDGTLAATAVQGSYQMGYLGVKTVA
                     EVLQGKKVPKMLDTGVVMVTKTNIDTPQAKNVLY"
     gene            11604..12626
                     /locus_tag="VF453_06775"
     CDS             11604..12626
                     /locus_tag="VF453_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7687389.1"
                     /translation="MKVTDRRTFADGRLEVTVFGLGCAALAGLFEAVPEPEARATLDA
                     AWDAGLRYFDTAPFYGYTRGERRVGAALVRRPRGELVLSTKVGRLMEPDDGVAAGSDG
                     WADPLPWRPRFDYTYDGVMRSLEQSLHRLGLERVDILLVHDIGRLTHGERHDLHWAQL
                     TRGGGLRALEELRRDGRIAAFGLGVNEFDVVRDAMQEARLDCTLLAGRHTLLEQRALD
                     FLDACARDGNAIIIGGPFNSGVLAGDPKFDYGAAPPDVVRRAQALADACAEFDVPLQA
                     AALQFPTAHPAVVSCVAGARTAAQLRQNVRWFEQEIPPALWAALRTRGLLDERAPCPG
                     DPGARS"
     gene            12620..13513
                     /locus_tag="VF453_06780"
     CDS             12620..13513
                     /locus_tag="VF453_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7687390.1"
                     /translation="MMMRIDAHQHFWRLADRAGQWPPAELVAIHRDFLPADLAVSLRD
                     AQVDGTVLVQSLPSEDDTRFLLALADAHPFVRGVVGWTDLKAPDAVQRIEALARHPRL
                     RGLRPMLQDLPDDDWIADPALAPAIDAMCRLGLVFDALVQPRHLRFLHAFARRFPALA
                     IVIDHAAKPFIARGELQPWRDDMAALAALPNVACKLSGLLTEAGADTHAEALRPYVQA
                     AWDLFGPSRLLWGSDWPVLRLAADYDGWLAMSEHLLATLREPADARARAAIFGGNAMR
                     LYRLAPPAPVATGRPPASLID"
     gene            13518..14528
                     /locus_tag="VF453_06785"
     CDS             13518..14528
                     /locus_tag="VF453_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006052099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX7687391.1"
                     /translation="MLTVICDTPGTLLARSRPVPERRAGEVLLRVKRVGVCGTDLHIF
                     TGNQPYLSYPRVMGHELSGIVEEADADSGLAPGDAAYVMPYLSCGRCIACRQGKTNCC
                     VAIEVLGVHRDGAFTEYLALPARFVHRADGVTLDQAAMVEFLAIGAHAVRRGDVRAGE
                     RVLVAGAGPIGLATMLFARLRGATVVALDGRRDRLDFALAHVGAAGAVAVGEDDEAEL
                     ARLSDGEHYDVVFDATGNAAAMERGFRFIAHGGRYVLVSIVQGRIGFSDPEFHKREAT
                     LLSSRNATDEDFATVLEAMRAGRVPDAALATHRLRLADVPATFAGLLDPAAGVIKAIV
                     EC"
     gene            14536..15669
                     /locus_tag="VF453_06790"
     CDS             14536..15669
                     /locus_tag="VF453_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017424712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEX7687392.1"
                     /translation="MKPGNPILQFGTGRFLQAHVGLFVSEALARGDALGGITVVQTTG
                     SPASTARTAAMAAGEGYEVRIRGLVDGRAVESSQRCRAVRTALVASAHWAAIREDVAD
                     GTVQVIVSKTGDDGWRPDPADGPALLDTAAAAPRAFPAKLLVLLHARWTRRPAAALTI
                     LPCELVARNGHALRDLVIAQAREWGLPPAFADHLRHGVTWANSLVDRIVSEALEPVGA
                     VAEPYALWAVERQQGLVLPCAHPAIVLTDDLGRHERLKLHLLNLGHTVLAERWLRTER
                     GSERTVLQAMAEPGYRGALESVWRDEVLPVFDAWGLASEAAAYVRSVRDRLLNPYLAH
                     RLADIAQNHAQKKARRLEPVVEAARVALPLLPQPALRAALAAD"
     gene            15688..16017
                     /locus_tag="VF453_06795"
     CDS             15688..16017
                     /locus_tag="VF453_06795"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEX7687393.1"
                     /translation="MQRMGMVIGIVPDRIGEYRRLHEAVWPAVLERLRASHIRNYTIF
                     LREPENLLFACWDYHGADFAADMAAIAADPATRRWWTFTDPCQQPLPTRTPGEHWAAM
                     ERVFHLD"
     gene            16048..17577
                     /locus_tag="VF453_06800"
     CDS             16048..17577
                     /locus_tag="VF453_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX7687394.1"
                     /translation="MTSAPALLLLHPSDSVAVARLALPAGASPALPDGRTVTASQAIP
                     AGHKIAVKDIAPGDTVRKYGQSIGVATRAIAAGEHVHVHNIDMGSSHPAHGVGRDYVP
                     TAPAARPATFEGYVRADGGVGTRNYVGVVSSVNCSATVCKAIAQAFAGDAMSPWPGVD
                     GVVAITHGGGCGMSGSGEGIELLRRTLRGYASHANFAGVLVVGLGCEVNQVGAIAAGL
                     GEAEPDRIATMTIQEAGGTRAAIEEGRRLVEGLLHRASAARRSPVSVSRLVVGLQCGG
                     SDGYSGISANPVLGAAVDLLVAHGGTAILSETPEIYGAEHLLTARAATPAVADRLLQR
                     IRWWEDYTARNGGDMNNNPSPGNKAGGITTILEKSLGAVAKGGGTGLMDVLEYAQPVR
                     VPGLVFMDTPGYDPVSATGQVAGGANLICFTTGRGSTYGCKPTPSLKLATNTPMFERM
                     RLDMDFNAGEILDGTLDVASAAERLFQLMLATASGRRSLSETNDLGDNEFVPWQLGAV
                     M"
     gene            17553..18530
                     /locus_tag="VF453_06805"
     CDS             17553..18530
                     /locus_tag="VF453_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX7687395.1"
                     /translation="MATGRGDVIEAPDRDILLLGEPLVLFAAADEGPLATVRRFDRFC
                     AGAELNVAIGLARLGLDVGYLSRVGDDSFGSHLCEVMDREGIDRTGVVVDPDRPTGFM
                     LKARAAPGDDPVIEYHRRGSAASRMSARDMPVAPGRRTRQLHLTGICPALSDSCRELA
                     FALAAATRAAGRRVSFDPNVRPHLWPDRDAMVATLGGLAALADLVLPGLEEGRLLTGR
                     QSPQEIATHYLDRGAQHVVVKLGPAGAFCASRGAPGVFVPGRPVERVVDTVGAGDAFA
                     TGVISGLLDGLGLAEATARGNVLGARAVRFPGDSDGLPTREELADELRR"
     gene            complement(18605..19294)
                     /locus_tag="VF453_06810"
     CDS             complement(18605..19294)
                     /locus_tag="VF453_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7687396.1"
                     /translation="MTNTAGRILVVEDEAELRRFVALSLGREGFDVHEAGGLERALIE
                     AASRRPDLVLLDLGLPDGDGVDFVRDLRTWSQVPVVVLSARGDERDKIEALDAGADDY
                     LIKPFGVGELLARVRAHLRRRIGQRPEQSVIEFGAIRIDIGARSVRNGGEAVRLTPIE
                     YRLLTHLATHPHAVLTHRQLLNAVWGPSHSQDTHYLRVYMGQLRKKIEADPAQPVHLL
                     TETGIGYRFVP"
     gene            complement(19291..>20874)
                     /locus_tag="VF453_06815"
     CDS             complement(19291..>20874)
                     /locus_tag="VF453_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX7687397.1"
                     /translation="GDAPTPAAGPRIGPTEDGRRPAWRAYAATLLVCAIAAAAAAPLS
                     AVFELSNVVMVFLLAVVFAAVRYGRGPAVLAAFVGVAAFDFLYVPPRLSFSVTDVQYL
                     VTFSVMLVVGLVIGQLTAVLKWQARVAMLREERVGALYRMSRDLSGALLLEQIAEIAA
                     RFLDSEFEARSALLVADLDDRVQEPLPSASAPGKVDRGIAQWAYDHGEPAGQGTHTLP
                     ASPVLYLPLKAPMRLRGVLAIEPRNPSRLAGPEQMRLLETCASLVAISLERVHYVDVA
                     RESTVHIESERLRNSLLAAISHDLRTPLAALVGMADTLALGGADAARQAGLAKDIRDA
                     SLRMSSQVANLLDMARLQSGHIRLSRQWLPLEEAIGTAMQAMSGVLDRRRVSVKLPPD
                     LPMLHVDPGLFDRVLCNLLENAQKHTPPGTPIEIGALESGDRIRILVEDRGPGLPRNR
                     EEAIFEMFERGRKESATPGVGLGLAICRAIVTAHDGTIVAENRPGGGARFVIDLPRVE
                     PPALDGIEAAADGDDGPRA"
BASE COUNT         3089 a         7629 c         7252 g         2904 t
ORIGIN      
        1 cgccgcagcg acgtcgccgg cgcccgtggc cccgctgccg acggccgaac tggcgccgga
       61 gcatccgccc atcaccgctg ccgcgccggc gcccgtcgaa cccgcgccga ccgctgcccc
      121 ggcgtccccc gtcgtcgcca cggaacgtgc gacgctgacg ctcacccaga tcaacgagcg
      181 cctgaacggc ccgaagctgg acgccgcctg ccttgccaag ctgggcgtgc cgcaggtgga
      241 atcgaaagga cccgccgtcc gcatcgacgc cgaagactgg gcgctcctga aggctgcgct
      301 ggtcgcccac atcgaggcgc tcgcatgaac cggcggcacc gcgcgcggcg cgccgtcgtc
      361 gcgcgcccga ccttcagcct tctggacgag gccatggcga gcaacacgga gccactcgcc
      421 cgcgcgcgcc gcgtcgagca gctcacgcgc atgtgggagg gcttcgacgc tatgtccacg
      481 gcctccatgc ccaccaccga cgactggcgg gtctgcagcg acgcggtgaa cctgatggag
      541 agcctcgtcg acctcggcta cgtcgaggac gggtcgaagc tcctcgagga cgccgtcgcc
      601 gccctcgccc gtgctggcca gcgcgccacc gatggaaagg cgctgcgact cgacggcccc
      661 ggccgcgacg cggtgcgcgc cgtccttctc gactacggct cggcgctgga gcttctgccc
      721 gcgcgcgcct tcatccagtg ccaccgccgc accgagcggc gcatccgcga gatcttggcc
      781 ggccgcggcc aggcgcacga catcgaagtc gtgagcctct gaccaccgaa agcgacacca
      841 tgaccacgac cacgccgcgg cgccgccgcg tcaagaagca acccgacgcc cgccccggcg
      901 tcgacatgct ggcttcccag ccgcctaccg caccggcgcc cgtgcgcctg cccaagcccc
      961 gcgggccggc caacatgccg ggcgagccca tcgtgaccga gcgcacgaag atcactcgct
     1021 gcccgtcgcc cagcctcggc ctgcacacca acacgcacgc cgaagcctga ctgagggccc
     1081 gacgccctca tgcgctattc ccaggacaag gacatcgcga agtcggtcgc cgagctgctc
     1141 cgcgagggct ggacgtacac gcggggcaac cgccatccgc gcgtcgtcgc gccgaacggc
     1201 aagttcgtgt cgttcagcgt cacgccctcg tgcccgcatg ccgcagcccg cttccagcgc
     1261 gatgtccaaa ccatcaaggc caccctgtga tcgtcatcga acaaccaaag gtcgaagaag
     1321 tccgtgacgc cgccggcgac atcgaaaggc gcctgacgca tcccgcgttc gcgctgattc
     1381 gcgccacgcg catgaccggc cactgcgccc tgtacggcac ggacttcatc cacaacaacc
     1441 tcgtcaagat cgcgatcggc cgaagcgcgc tgcatcggtc gacgtcccat gactggcccg
     1501 cgccgggcgc cgagctggtc gaagtcgcga tgagcgaagc gcagtgggcg cggttgatct
     1561 cgtccaccgg cggagtcgac gcggagacgc cctgcacgct ggtcaagctc ggtaccgaca
     1621 ccgttccggg actcccgcca ccagagtcgc gtgccgacga attcgctgac gagctgcgaa
     1681 acacgcagtc ccgggctatc agttccctcg gcgacttgcg cgctgcgatc gatgggcttg
     1741 gcctctccaa gcgctcggcc gcaagcctcg cggcgatggt cgacaacgcg ctgacgcaga
     1801 tcaccgagaa cagcgagcat gtggtgcgcc agttcggcaa gcacgccgag cacctcgtcg
     1861 aggaggtcaa gaccgaggtc agcgcctgga tgcagaccat gatcgccagc gcccaggccg
     1921 cggcccgcct gggcgaccgg tcggcgccgc cgctgccgcc gctcgagctg aacggcaacg
     1981 ggaacggcaa gtgaccgcgg ccgccgaatc cagctgtcgc gcctgcggcc acgcgccaat
     2041 cgacggctgc tcgcgcgtcg agtgccccga gaggaaggcc tggagcgccg gcgcgctcga
     2101 cgacctggcg cccgccgcgc actcgggcgt cttcagcacc aacggccagg acggccacgt
     2161 gcaggtcggc acggccggct gcttccgccg cgtgaaacac cgcggcgcgg aatgaacagg
     2221 aggacaacca tgtccatcaa catcgagaag ttcgccggcg tcgacgactt ccgcacctac
     2281 atcaaccggc cgtggcgccg agacagcaag ctgtacgcga cgaacggtca cttcatgatc
     2341 gagatcgacg acgacggccg cgacgccgcg ccgttcgaga agcagccgga ctgcgtcggg
     2401 ctcttcgcca agtacacgcc gggcgatttc atgccgattc cgccgctggc cgcaccggtt
     2461 cactgccagt ggtgcaaggg ccgcggcgcc tgctatcgca aagcgtgccc ggactgcgac
     2521 ggccagggcg agttcgccca cggcatgtac gactacgact gcaagcgctg cgaaggcgac
     2581 gggtacttcc tgttcccgac cgccccgccg ggcgagaagg agacgcgctg ctccgcctgc
     2641 tacggcctcg gtgaagaggt gcgcaaggat caaggaacgc cggtcggaga acttctcgtc
     2701 gccacgcgcc tgctgcgcgt gctcaccgag ctccccggca tcgaggtcgc cgtctgcaag
     2761 gctcctgaag cgctctggtt ccgattcacc ggcggccgcg ggctggtgat gccgatgcgg
     2821 aagggctgag cgatgtcgac tcccccgaaa gacttcggcc cgctcagcaa cggcgacctg
     2881 acccgcattg cggagtccgt cggcatggtc aggagcttcg atggcgtcct gatgcgcagc
     2941 atcggccacg ccggcggcat gacggcggag cacggcttcg cattcgcacg cgccgcgatc
     3001 tcggaagatc gcgcccgcca agcggcggcg gcgccgaagc cggcgggcgc cctcgacgag
     3061 aacatcgctc tccgcgagat ggccaagcac gcgcaccgcg cgctgaacgc cgcggccgca
     3121 atcctggcca ccatcgacga agaggagctg ggcgaccagc gtgtcgccga cgtggagaag
     3181 cgctgcacgc agatcgcctg cagcctgttc tcgctgctgc gcgggaacga ggtcgaccgc
     3241 tacgagtcgt ctccgctgga aactgcagcg cttcccttgg cagggacgag aggtgccgca
     3301 tgaagctcgc cgaccgtttc atagcgcgcg tgacgcgccg gccgcccgac ttcgtgatcg
     3361 gtggcgccgc gcgcccctac ctgcgccgct ggtgggtgat cccgcggaac cggctgttca
     3421 acgtctactt gcaccagttc ctccgcagcg acgacgaccg cgcgctccac gaccatccgt
     3481 gggccaatgc gtcgatcctg ctgcgcgggc ggtatgtcga gcacacgatc tccgccggcg
     3541 gcatcgagca tcgacgcgaa ctgcgcgccg gcgacttccg cctacggccg tccggccgct
     3601 tcgcgcaccg cgtcgagctg gtcgatgggc cgtgctggac gctgttcctc accgggccgc
     3661 gctaccgtga atggggcttc cattgccccc gcgccggctg gattcactgg gagaaattca
     3721 ccgcggccga cgacgtcggc gccgtcggga ggggatgcga ccaatgacga aacgttctgc
     3781 taccgccatg accgaaccga gcgccggccc agcttcccat gcaccgagcg agctgaggcc
     3841 cttggtcgtc ccgctcgagt ggatcaagct cgaccgctac tgcgagctca ccggcgatac
     3901 gccgggcgcc gtccacgcac gccgccgaaa gcgacaatgg tcgaacggcg tggagtgcat
     3961 cgtcggcccg gatggcaatc tctggatcaa tccggcagcg ttcaacgcct gggtgcgccg
     4021 cgagaaggcg ccggagacga cgtgaccgcg ccgaagaagc tgaaggagat cacgctgccc
     4081 cgcggcgtga agatccgcga gttcgccacc gagcggcgcc tgcaggtggc cttcagctac
     4141 cgcggcgagg aatgccgaga gctcctgccg ccacagccca tcacacagac caccgccaac
     4201 ctggctggcg gcctgcgcca ggagatccag cgcaagatcg tcgaagggtc gttccattac
     4261 gccgactact tcccgggcag cgcccgcgcc gcgcggttca gcgcgggcac atctcgcgcg
     4321 ctcatcacgg acaagctcga ggcccagcgc gagatctacc gccggcaggt gaaggaaggc
     4381 cggatgtcgc cgagcaccct gcacggctac gagaaggcga tcgactcggc gaagtacagg
     4441 tactgggagg gaaagacgct cgctgacgcc acgcccgcgg cgctgcgcga gtggatcggc
     4501 ggcttcggcg tcacggccaa gttcgcgcgc aacctgctca tcccgatgcg cagcctgatg
     4561 gaggacgcgc tgaacgacga cctgatcgac gaagacccgt tcaaccggat cgccctgaac
     4621 aagctgctgc ggcagaccgc ggccgacagc gactaccacg tcgacccata cagcgccgac
     4681 gaacgagcag cgctcctggc agcggcccga gccgatgagc gccctctcgt ggcattttgg
     4741 ttcgagaccg ggctgcggcc cggcgagctc ttcgccctcc ggaagacgag gatcctgcga
     4801 gaacgaacgg tcgcccgcgt cgacacgaac tgggtgcaga acacggagaa ggcgccgaag
     4861 accgccgccg ggaaccgcgc cgtcgacctg agctcggccg ccctgggcgc ggtcgaggcc
     4921 cagctcgaga tcacgccgca ggccatcgag cacctcttca tcaacccgac gaccggccgg
     4981 ccgtgggaga acgacacgca gctccgcaag agcctgtgga tcccgctcaa cgaacgcgcg
     5041 aaggtgcgct accgcaaccc ctaccaggct cggcacacct tcgccagcac gcggctcaca
     5101 gccgggcaca atccctggta cgtcgcgcag cagctcggac atgcgaacgt ccagatggtg
     5161 ttccaggtct acggcaagtt catctcggac gactaccagc cgcccaaggc cgccgccaaa
     5221 ggcgacgggc gctgaatcgc acgcgattcg cacgtagcgg gctgcgagtc gcaccaggac
     5281 tcaaaatcac gcgggttcaa ttccccccgg ctccaccacc tacctgccca gcgacctccg
     5341 ctggcgtcca aagacccaag gcgaatcgac ggcttgggtc tttttgcttg tccgccaggc
     5401 gtggctaacg ccccgccatc ctgcagcgct gccgctgctc gccgagcccg gcgatgccaa
     5461 ggcggacgac gtcgccgggc gccaggtagg tcggcggatt ctggcccagg ccgacgccgg
     5521 ggggcgtgcc ggtcgagatg atgtcgcccg gctgcaggct catgaaccgg ctcaggtagc
     5581 tgacgatgtg cgcgacaccg aacaccatgg tgcgggtgtt gccgctctgg tagcgccgcc
     5641 cgttgacctc gagccagagg tcgagcgcct gcgggtcggg gatctcgtcg gcggtcacca
     5701 gccaggggcc gatcggtccg aaggtgtcgc agcccttgcc cttgtcccac tggccgccgc
     5761 gttcgagctg gtattcccgc tccgaaacgt cgttgaccac gcagtagccg gccacgtggc
     5821 gcatggcgtc gcgctcgtcg atggcccggc cgccctcgcc gatcaccacg ccgagttcga
     5881 cctcccagtc ggtcttggtc gatcccggcg gaatctcgac atcgtcgtcc ggccccgtga
     5941 tggcgctcgt ccacttgccg aacaccacgg gctcggtcgg gatcggcatg cccgattccg
     6001 ctgcgtggtc ggcgtagttc aggccgatgc agatgaactt gccgacgcct gccacgcagg
     6061 ggcccagccg caggtcgtcc tggggacggc cgctgacgag cggcagcgac gacggatcga
     6121 gcgcgcgcag ccggtcgagt cccgcgggcg tcaggacgtc gccgcggaga tcgggcacga
     6181 ccgcgccgag gtcgcggacg cggccctggg cgtcgagcag gccggggcgc tcgttgccgc
     6241 gggtcccata tcgaagcaat ctcattggcg gctcctgccg gtcagatggt gacgccgccg
     6301 tcgaccgaca gggcctggcc cgtcacgaag cgcgattcgt cgctggccag gtagaccagc
     6361 agcggtgtga tgtcgtcgac ctccgcgagg cggcccatgg gctggcgggc gatgaagtcg
     6421 cgctcggcct gcgccggatc ggccgccgcc gcgatgcggc cgcgcagcga gggcgtgtcg
     6481 acggtgccgg ggcacacggc gttgcagcga atgccctggc ggacgaagtc ggccgcgatg
     6541 gacttcgtca gcccgatcac tgcggccttg ctcgccccgt agacgcagcg gttggcgaac
     6601 cccttgatgc tcgaggccat cgaggacatg ttgatgacgc tgccgccgcc cgccgccagc
     6661 atgccgggca aggccgcgcg cgtgacccgg tgcatgctgg cgacgttgag gtccatgctg
     6721 cgcgcccagg cgtcgtcgtc gcactcgagc agcgagccgt ggtgcacgat gcccgcgcag
     6781 ttgaagagga cgtcgatgcg ccctgccccg gcgaccgcgg cgttgacggc ggcggtgtcg
     6841 cgcacgtcca gcacccgcgt gcggatgagc ccgtgtccga gcgtggcgag cgactcgggc
     6901 gcgatgtcgg tcgcccagac ggtggcgcct tcggcggcga acgcgagcgc cgccgcgcgc
     6961 ccgatgccgt tgccggcggc ggtgacgaag gcggtcctgc cctgcaatct ctggctcatg
     7021 actttccttt cggaggcgct gccggaggcc gcgctcattt gcccggctcg tcggtcgccg
     7081 tgccgcccac ccaccgcgcc tgcgacgccc gcaggtgcat gcccatcgcg gcggcggcgg
     7141 cctggggatc gcgcgcatcg agcgcttcgt agatgcgttc gtgctcggcc agcgcctcgc
     7201 tccacgtcgc gacgttctcg gagcggacgc tcagccggga ggcgatcggc ccgtgccgtt
     7261 cgtcgaacag ctcgcccacc aggcgcacca gcaccgagtt gccgcccatc tcggcgatgg
     7321 cgacatggaa gcggcgatcg ttttccagcg gcccgcgccc gcgcgcatgg tcgtcgcgca
     7381 tcgcgtcgag gatgccgcgg atgcgctcca tgcccgggcg ggtgacgcgc gcggcggcca
     7441 ggacgatcac cgccgactcg acggcggcgc gcgcgtgcat cagctcggac gggctttcgc
     7501 cgagcgcggg cgtgtggtcg gcgcgtcgcg ccggtgcgca gacgtagacg ccggagccca
     7561 tgcgaatctc gacccggccg tcgatctcga gcgcgatcaa cgcctcccgc agcgagggcc
     7621 gcgacacgcc cagctgcagc gccagctcgc gctccggagg caggcggctg ccgaccgcgt
     7681 aggcgccatc cgcgatgacc gcccggatct ggtcggcgac ctgctggtac agccggcggg
     7741 tatcgctctg cctgacaggg ggcatgtgaa ctccagaagc aattggccag accagacgag
     7801 aaagcgcgga tcggggccag cgagcgcgcg agaggctcga ctgtaccggg atcgtcactg
     7861 cgagggattc gggtttgtat tggcctgacc agatgcaaat tagtggcaca gtggcctgac
     7921 agcattggtc tgtccaattt gccgagcttc gcggttccct cctgtcgcca tgacttgtcc
     7981 cgcccgccct tctccgactc gccgcatctc ctgccggagg tccgtcgcat gagcgatgcc
     8041 gcgtccgccc cactgctgga gctggacggc gtctgcaagc gcttcctggg cgtcgtcgcg
     8101 ctggaaggcg tcagcttcga cctgcggccc ggcgaagtgc acgcggtctg cggcgagaac
     8161 ggcgcgggca agtcgacgct gatgaaggtg atcagcggcg agtaccaggc cgacggcggc
     8221 acgatccggt accgcggccg cgaagtgcgc ttcccgtcgc cgctcgccgc gcagcgccag
     8281 ggcatcgtga tgatccacca ggagctgaac ctggtgcctc acctgtcggt ggccgagaac
     8341 atctacctgg cgcgcgagcc gcgccgcgga tggttcgtcg atcgggcgcg cctgcgcgcg
     8401 gatgcccagg ccaacctcga ccggctgggc ggcgggatct ctccggacgc caccgtcctc
     8461 agcctgccgg tcgcgcagca gcagatggtg gagatcgcca aggcgctgtc gatgcgcgcg
     8521 tcggtgctca tcatggacga accgacctcg tcgctcaccg agacggagac cacgcggctt
     8581 ttcaaggtca tccatgagct caagcgcgcg ggggtggcca tcgtctacat ctcgcaccga
     8641 ctcgaggaga tggccgagat cgtggaccgg gtgaccgtgc tgcgcgacgg ccgctacatc
     8701 tcgacgatgc ccttcgccgg gacgagcgtg gacgacattg tggcgcgcat ggtcggccgc
     8761 gcgctcgacg acaagttccc gccgcccacg cggcagcccg ccgacgaggt gctgctgtcg
     8821 gtgcaggagc ttcggcgcga gggacgcttc gggccggtca gcttcgagct gcggcggggc
     8881 gagatcctgg gcttcgcggg actgatcggc gccgggcgca ccgaggtggc gcgcgccgtc
     8941 ttcggtgccg atccggtcga cggcggcagc gtcaggctga acggtcgcgc gctcgcgatc
     9001 cgctcgccga tcgacgccat ccgccacggc atcgcctacc tgtcggaaga ccgcaaggcg
     9061 cacggcctgg ccgtgaagat gacgctggcg cacaacgtca ccctggccaa catgcatgcg
     9121 gtctcgggac ggcgcggatt catccgcttc gcgcaggaga tcgccgtcgc gcgccgctac
     9181 atcgacgcgc tgggcatccg cacgcccggg ccgcggcagg tcgcgcggct gctgtcgggc
     9241 ggcaaccagc agaaggtggt gatcgccaag tggctgttcc ggcagtcgca ggtcatcttc
     9301 ttcgacgagc cgacgcgcgg catcgacgtc ggggccaagt acgcgatcta cgagctgatg
     9361 gacaggctcg ccgcgcaggg ggtgggcgtc gtcttgatca gctcggagct gcccgagatc
     9421 ctcggcctca ccgaccgggt ggccgtgttc cacgagggac ggctcgccag cgtgctcgag
     9481 acgggccgca cgacccagga agaaatcatg cgccacgcct ccggatccgg cgccgccggc
     9541 cgtgcgtcca cggtcccgcc ggacctcgac ctgaacctcg tcgccccatg aacgatcgcc
     9601 agaaagacct cgtccagaag ttcgccgcgc tcgccggcct gctggtgctc gccaccgtct
     9661 tctcgctgac cagccgcgcc ttcctcaccg tcgacaacgg catgacggtc gcgctgcagg
     9721 tcacgtcgat cgcctacctg ggcatcggcg ccacgttcgt catcatcacc ggcggcatcg
     9781 acctgtccgt gggctcggtg ctcgccctgt ccggcatcgt cgccgcgctg tgcgtgaagt
     9841 cgggcatgcc ggtggcgctg ggcatgtcgg cgggcgtcct cgccggcacg ctgtgcggag
     9901 cgatcaacgg cctgctggtc acgcgcctga agctgccgcc gttcatcgcc acgctcggga
     9961 tgatgctggt cgcccgcggc gtcgcgctgc aggtcaccgg cgcgcgcgcg atctcggggc
    10021 tgggcgacgc gttcggcgaa ctcggcaacg gctcgctggg ccgggtcgtc cgggtggacg
    10081 cgagcggctt tcccgacatc gtctttcccg gcataccgta ccccgtactc atgatggtgg
    10141 tgctcgcggt ggtcgcgtcg gtcgtgctga cgcgcacgac cctcggccgc catgtctacg
    10201 ccgtcggctc caacgcggag gcggcgcgtc tgtcgggggt caacgtctcg ggcgtcgtgc
    10261 tgttcaccta cgtcgtctcc ggcgcgcttg ccggcctggc gggctgcgtc ctgatgtcgc
    10321 ggctggtcac cggccagccc aacgaaggcg tgatgtacga gctggacgcg atcgcggcct
    10381 cggtgatcgg cggtacctcg ctgatcggcg gcgtcggcac gatctcgggc accgcgatcg
    10441 gctcgttcgt gatcggcatc ctgcgcaacg gcctgaacat gaacggtgtc tccgccttca
    10501 cgcagcagat cgtcatcggc ctggtcatcc tgctcaccgt gtgggtcgac cagatgcgca
    10561 accgccgatg agcccggccg gcccggcgca gggccggccc tttccacacc aagaacccac
    10621 tggagacaag acatgagcaa gatgcttccc cgattggtgc tcctcaccag cctcgcgatg
    10681 gccgcagcga ctgcgtgcgc cggcgagatc gccgtgatcg tgaagaccac caactccacc
    10741 tactggcaga acgtcaacaa gggcgccagc gccgccatca aggagatccc gggccgcacc
    10801 atgaccttct cggggccggc ctcggagtcg gccattgccg acgaggtgaa ccttgtcgag
    10861 aacgcggtga accgccacgt ggacgcgatc gtgctcgccg cctccgatcc cgacgcgctg
    10921 gtgccggcga tcaagaaggc ctgggaagcg cggatccccg tcgtgctgat cgactcgatg
    10981 gtgtccgacg ccggcaagcc gtactaccag tccttcctct cgaccgacaa caagctggcg
    11041 ggcgagctgt gcgcgaagca gctgatcgcc gatgtcggga cgaccggcaa gatcgcgatc
    11101 atgtcctacg tcgccggggc cggctccgag gtgggacgcg tcggcggctt caaggactac
    11161 atcgcggcgc actcgaagct gcagatcgtc gggccgttct attcgcagtc gcagatggcc
    11221 acggccctca accagacgac cgacgtgctc gcgtccaacc cggacctgaa gggcatcttc
    11281 ggcgccaacg agccgaccgc ggtcggcatg gggcgggcgc tcgtccagtc gggcaaggcg
    11341 ggcaaggtcg tggccgtcgg cttcgacggc aacgaggacc tgcagaagtt cgtgcgcgac
    11401 ggcacgctgg ccgcgacggc ggtgcagggt tcataccaga tggggtatct cggcgtgaag
    11461 acggtcgcgg aggtgctgca gggcaagaag gtgcccaaga tgctggacac cggcgtggtc
    11521 atggtcacca agaccaacat cgacacgccc caggcgaaga acgtcctgta ctgatcggcg
    11581 cgcacgccag aacgcggctg cacatgaaag tcaccgaccg ccgaaccttc gccgacggac
    11641 gcctcgaggt gaccgtcttc ggcctgggct gcgccgcgct cgcggggctg ttcgaggcag
    11701 tccccgaacc ggaagcccgg gcgacgctgg acgccgcctg ggacgccggc ctgcgctact
    11761 tcgacacggc gccgttctac ggctacacgc gcggcgagcg gcgcgtgggc gcggcgctgg
    11821 tgcggcggcc gcgcggcgaa ttggtcctca gcaccaaggt cgggcgcctg atggaacccg
    11881 acgatggcgt ggccgccggc agcgacggct gggcggatcc cctgccctgg cgcccgcgct
    11941 tcgactacac ctacgacggc gtgatgcgct cgctcgagca gagcctgcac cggctcggcc
    12001 tggaacgggt cgacatcctg ctggtgcacg acatcggccg tctcacgcac ggcgagcgcc
    12061 acgacctcca ctgggcgcag ctcacccgcg gcggaggctt gcgcgcgctc gaggagctgc
    12121 gccgcgacgg ccgcatcgcg gcgttcgggc tgggcgtcaa cgaattcgac gtggtgcgcg
    12181 acgcgatgca ggaggcccgc ctggactgca cgctgctggc cggccgccac acgctgctcg
    12241 aacagcgtgc gctggatttc ctcgacgcct gcgcgcgcga cggcaacgcc atcatcatcg
    12301 gcggcccgtt caactcgggc gtgctggcgg gcgatccgaa gttcgactac ggcgccgcgc
    12361 cgcccgacgt cgtgcggcgg gcgcaggcgc tcgccgacgc ctgcgcggaa ttcgacgtgc
    12421 ccctgcaggc cgcggcactg caattcccga ccgcgcatcc ggcggtcgtc tcgtgcgtgg
    12481 ccggcgcgcg cacggccgcg cagctgcggc agaacgtgcg ctggttcgag caggagatcc
    12541 cgcccgcgct gtgggcggca ctgcgcacgc gcggcctgct cgacgagcga gcaccgtgtc
    12601 cgggcgaccc gggggcacga tcatgatgcg gatcgacgcg caccagcact tctggcgcct
    12661 ggccgaccgc gccggacagt ggccgcccgc ggaactggtc gcgatccatc gcgacttcct
    12721 ccccgcggat ctcgccgtgt cgctgcgcga tgcgcaggtc gatggcaccg tgctcgtcca
    12781 gtcgctgccg agcgaggacg acacgcgctt cctgctcgcg ctggcggacg cccatccgtt
    12841 cgtgcgcggc gtcgtcggat ggacggacct gaaggcgccc gacgccgtgc aacgcatcga
    12901 ggcgctggcg cgccatccgc ggctcagggg cttgcggccg atgctgcagg acctgcccga
    12961 cgacgactgg atcgccgatc ccgcgctcgc gcccgccatc gacgcgatgt gccggctcgg
    13021 cctggtgttc gacgccctcg tgcagccgcg ccacctgcgc ttcctgcacg cgttcgcgcg
    13081 gcgcttcccg gcgctcgcca tcgtcatcga ccatgccgcc aagccgttca tcgcgcgcgg
    13141 ggaactccag ccctggcgcg acgacatggc cgcgctcgcg gcgctgccca acgtcgcctg
    13201 caagctctcg ggcctgctca ccgaagccgg cgccgacacg cacgccgagg cgctgcggcc
    13261 ctacgtgcag gccgcgtggg acctgttcgg cccgtcgcgg ctgctctggg gcagcgactg
    13321 gccggtgctg cggctggccg ccgactacga cggctggctc gcgatgagcg agcacctgct
    13381 cgccacgctg cgcgagcccg ccgacgcccg cgcgcgggcc gcgatcttcg gcggcaacgc
    13441 gatgcgtctc taccgactgg caccacccgc cccggtggcg accggccggc ctcccgcctc
    13501 cctcatcgac tgaacccatg ctcaccgtca tctgcgatac gcccggcacc ctgctggccc
    13561 ggagtcgccc ggttcccgaa cgccgggccg gcgaagtgct gctgcgcgtc aagcgcgtcg
    13621 gcgtctgcgg caccgacctg cacatcttca cgggcaacca gccctatctg tcctacccgc
    13681 gcgtgatggg ccacgagctg tccgggatcg tggaggaggc cgacgccgac agcggcctcg
    13741 cgcccggcga cgccgcctac gtgatgccgt acctgtcgtg cggccgctgc atcgcctgcc
    13801 ggcaaggcaa gaccaactgc tgcgtcgcga tcgaggtgct gggcgtgcat cgggacggcg
    13861 ccttcaccga gtacctggcc ctgcccgcgc gcttcgtgca ccgggccgat ggcgtcacgc
    13921 tggaccaggc cgcgatggtc gagttcctcg ccatcggcgc gcacgccgtg cgacgcggcg
    13981 acgtgcgggc cggcgagcgc gtgctggtcg ccggcgccgg ccccatcggc ctggccacga
    14041 tgctcttcgc gcgcctgcgc ggggcgacgg tggtggcgct cgacggtcgc cgcgaccgcc
    14101 tcgacttcgc gctcgcccac gtcggcgcgg ccggcgccgt ggcggtcggc gaggacgacg
    14161 aggccgagct cgcgcggctc agcgacggcg agcactacga cgtcgtgttc gacgccaccg
    14221 gcaacgccgc ggcgatggag cgcgggttcc gcttcatcgc gcacggcgga cgctatgtcc
    14281 tcgtgtcgat cgtgcagggc cggatcggct tctcggatcc ggaattccac aagcgcgagg
    14341 cgaccctcct gagcagccgc aacgcgaccg acgaggactt cgcgacggtg ctggaggcga
    14401 tgcgcgccgg gcgcgtcccc gacgcggcgc tcgccaccca ccgcctgcgc ctggccgacg
    14461 tgccggcgac cttcgccggc ctgctcgatc cggccgccgg cgtgatcaag gcgatcgtgg
    14521 aatgctgagg cgcccatgaa gccgggaaac ccgatcctgc agttcggcac ggggcgcttc
    14581 ctgcaggccc acgtcggcct gttcgtctcc gaagcgctcg cgcggggcga cgcgctgggc
    14641 ggcatcacgg tggtccagac gacgggcagc cccgccagca ccgcgcgcac ggccgcgatg
    14701 gccgcgggtg agggctacga agtccgcatc cgcggcctgg tcgacgggcg cgcggtggaa
    14761 tccagccagc gctgccgcgc ggtgcgcacc gcgctggtcg cgagcgcgca ctgggcagcg
    14821 atccgcgagg atgtcgccga cggcacggtg caggtcatcg tgtccaaaac gggcgacgac
    14881 ggctggcggc ccgatccggc cgacgggccc gcgctcctgg acacggcggc cgccgcgccg
    14941 cgcgccttcc cggccaagct gctcgtgctg ctgcacgcgc gctggacgcg ccgtcccgcg
    15001 gccgcactca ccatcctgcc gtgcgagctc gtcgcgcgca acggccacgc gctgcgcgac
    15061 ctcgtcatcg cccaggcgcg cgaatggggc ctgccgccgg cgttcgccga ccacctgcgc
    15121 cacggcgtga catgggcgaa ctcgctggtc gatcgcatcg tcagcgaggc gctggagccg
    15181 gtgggcgccg tcgccgagcc gtacgcgctg tgggcggtgg aacgccagca gggcctggtg
    15241 ctgccatgcg cgcaccccgc catcgtcctg accgatgacc tgggccggca cgagcggctg
    15301 aagctgcacc tgctcaacct ggggcacacg gtcctcgccg agcgctggct gcgcaccgag
    15361 cgcgggagcg agaggacggt cttgcaggcg atggcagagc cgggctaccg aggtgcgctg
    15421 gaatcggtgt ggcgcgacga ggtgctgccg gtgttcgacg cctggggcct ggcctcggaa
    15481 gccgccgcct acgtgcgctc cgtgcgcgac cggctgctca atccctacct ggcccatcgg
    15541 ctcgccgaca tcgcgcagaa ccatgcgcag aagaaggcgc ggcggctcga acccgtggtc
    15601 gaggccgccc gcgtcgccct gccgctgctg ccgcagcccg cgctgcgcgc cgcgctcgcg
    15661 gccgactgac cccaaaggag accttcgatg caacgcatgg gcatggtcat cggcatcgtg
    15721 ccggaccgca tcggcgaata ccgccggctg catgaagccg tgtggccggc ggtgctcgag
    15781 cgcctgcggg cgtcccacat ccgcaactac accatcttcc tgcgcgagcc ggaaaacctg
    15841 ctgttcgcct gctgggacta ccacggcgcc gatttcgccg cggacatggc cgcgatcgcc
    15901 gccgatcccg cgacgcgacg ctggtggacc ttcaccgacc cgtgccagca gcccctgccg
    15961 acgcggacgc ccggcgagca ttgggcagcg atggagcgcg tcttccacct cgattgaccc
    16021 cgcgcccgcg cgcgacggcg acacaccatg acctccgcac ctgccctgct cctgctccat
    16081 ccctccgaca gcgtcgcggt cgcgcgcctg gccctgccgg ccggcgcgtc gcccgcgctg
    16141 ccggacggtc gcaccgtgac cgcgtcgcaa gcgatcccgg ccggccacaa gatcgccgtg
    16201 aaggacatcg cgcccggcga caccgtgcgc aagtacggcc agtcgatcgg cgtggccacg
    16261 cgcgccatcg ccgccggcga gcatgtccat gtgcacaaca tcgacatggg gtcgtcccat
    16321 ccggcgcacg gcgtcgggcg cgactacgtg cccaccgccc cggccgcccg gccggcgacc
    16381 ttcgaaggtt acgtgcgtgc ggacggcggc gtcggcacgc gcaactacgt cggcgtcgtc
    16441 tcgagcgtca actgctcggc caccgtgtgc aaggcgatcg cccaggcgtt cgcgggcgac
    16501 gccatgtcgc cctggccggg cgtcgacggc gtggtggcca tcacgcacgg cggcggctgc
    16561 ggcatgagcg ggagcgggga aggcatcgag ctcctgcgcc ggacgctgag gggctacgcc
    16621 agccatgcga acttcgccgg cgtgctggtc gtcggcctgg gctgcgaggt caaccaggtg
    16681 ggcgccatcg cggccggact gggcgaggcc gagcccgacc gcatcgccac gatgacgatc
    16741 caggaggccg gtggcacgcg cgcggccatc gaggagggcc gccgcctcgt cgagggcctg
    16801 ctgcaccggg cgagcgccgc gcggcgctcc cccgtctcgg tgagccggct ggtcgtcggc
    16861 ctgcagtgcg gcggatccga cggctactcg ggcatcagcg ccaatccggt gctcggggcg
    16921 gcggtcgatc tgctcgtcgc gcatggcggc acggccatcc tgtccgagac gcccgagatc
    16981 tatggcgccg agcacctgct gaccgcgcgc gcggcgacgc cggccgtcgc ggaccggctg
    17041 ctgcagcgca tccgctggtg ggaggactac accgccagga acggcggcga catgaacaac
    17101 aacccgtcgc cgggcaacaa ggccggcggc atcaccacca tcctcgagaa gtcgctgggc
    17161 gcggtcgcca agggcggcgg cacgggtctg atggacgtcc tcgaatacgc gcagcccgtg
    17221 cgcgtccccg ggctcgtgtt catggacacg ccgggctacg accccgtgtc ggccaccggc
    17281 caggtggccg gcggcgcgaa cctgatctgc ttcacgaccg gccgcggctc gacctacggc
    17341 tgcaagccga cgccctcgct gaagctggcg accaacacgc cgatgttcga gcgcatgcgg
    17401 ctggacatgg acttcaacgc cggcgagatc ctcgatggca cgctcgacgt cgcttcggcc
    17461 gcggagcgcc tgttccagct catgctcgcc accgcctcgg gccggcgcag cctgagcgag
    17521 acgaacgacc tcggcgacaa cgagttcgtg ccgtggcaac tgggcgcggt gatgtgatcg
    17581 aggcccccga ccgcgacatc ctgctgctcg gcgagccgct cgtgctgttc gcggcggcgg
    17641 acgagggccc gctcgcgacg gtgcggcgct tcgaccgctt ctgcgccggc gcggagctca
    17701 acgtggcgat cggcctggcg cggctgggcc tggacgtcgg ctacctgtcg cgcgtgggcg
    17761 acgacagctt cggatcgcat ctgtgcgaag tcatggatcg cgagggcatc gatcgcaccg
    17821 gcgtcgtcgt cgacccggac cgtcccaccg gcttcatgct caaggcgcgc gcggcgcccg
    17881 gggacgaccc ggtcatcgaa taccaccggc gcgggtcggc ggccagccgc atgagcgctc
    17941 gcgacatgcc ggtcgcgccg ggcaggcgca cgcgccagtt gcatctgacc ggcatctgtc
    18001 ccgcgctgtc cgactcctgc cgcgagctgg ccttcgcgct cgccgccgcg acccgcgctg
    18061 ccgggcgacg cgtgtccttc gatcccaacg tacggccgca cctgtggccg gaccgggacg
    18121 ccatggtcgc cacgctgggc ggcctcgcgg cgctggccga cctggtgctg cccgggctcg
    18181 aggaagggcg cctcctgacg ggccgccagt cgccgcagga gatcgcgacc cactacctcg
    18241 accgcggcgc gcagcacgtc gtggtcaagc tcgggcccgc aggcgccttc tgcgcgagcc
    18301 ggggagcgcc gggcgtgttc gtccccgggc ggccggtcga acgcgtggtc gataccgtcg
    18361 gcgccggcga cgcattcgcc acgggcgtga tcagcggact cctcgatggc ctgggcctgg
    18421 ccgaggcgac ggcgcgcggg aacgtgctcg gggcccgcgc cgtccgattc ccgggcgaca
    18481 gcgacggact gccgacgcgc gaggagctcg cggacgagtt gcggcgatag cgccgccctg
    18541 cgccgcgcgg cgacaaaccc gggccgcgga ttcgaacgct ggcggcgcgt cccccgtcac
    18601 gcgtctacgg cacgaaccga tagccgatgc ccgtctccgt gagcaggtgc accggctgcg
    18661 ccgggtccgc ctcgatcttc ttgcgcagct gccccatgta gacgcgcaag tagtgcgtgt
    18721 cctgcgaatg gctcggcccc cagacggcgt tgagcagctg gcggtgcgta agcacggcat
    18781 gcgggtgcgt ggccaggtgc gtgagcagcc ggtactcgat cggcgtcagg cgcacggcct
    18841 cgccgccgtt gcgcaccgac cgcgcgccga tgtcgatgcg gatcgccccg aactcgatga
    18901 ccgattgctc ggggcgctgc ccgatgcgcc ggcgcaggtg cgcccgcacg cgcgccagca
    18961 gctcgccgac gccgaagggc ttgatcaggt agtcgtccgc gccggcgtcc agcgcctcga
    19021 tcttgtcgcg ctcgtcgccg cgcgccgaca gcaccaccac cggcacctgc gaccaggtgc
    19081 gcaggtcgcg cacgaagtcg acgccgtccc cgtccggcag gccgaggtcg agcagcacga
    19141 ggtcgggacg gcgcgacgcc gcctcgatca acgcgcgctc gaggcccccg gcctcgtgga
    19201 cgtcgaagcc ctcgcgcccg agcgagagcg cgacgaagcg ccggagctcc gcctcgtcct
    19261 cgaccaccag gatcctgccg gcggtgttcg tcatgcccgg gggccgtcgt cgccgtcggc
    19321 ggcggcctcg atcccgtcga gcgcgggggg ctccacgcgg ggcaggtcga tcacgaagcg
    19381 cgcgccgccg cccgggcggt tctcggcgac gatcgtgccg tcgtgcgccg tcacgatcgc
    19441 cctgcagatc gccaggccca ggccgacacc cggcgtggcg ctttccttgc gcccgcgctc
    19501 gaacatctcg aagatcgcct cctcgcgatt cctgggcagc cccgggccgc gatcctccac
    19561 caggatgcgg atgcggtcgc ccgactccag cgcgccgatc tcgatcggag tacccggcgg
    19621 ggtatgcttc tgcgcgttct cgagcaggtt gcacagcacg cggtcgaaca ggccgggatc
    19681 gacgtgcagc atcggcaggt ccggcggcag cttcacgctg acgcggcggc ggtcgagcac
    19741 ccccgacatc gcctgcatcg ccgtgccgat cgcctcctcg agcggcagcc actggcggct
    19801 caggcggatg tgtccggact gcagccgcgc catgtccagc aggttggcga cctgcgagct
    19861 catgcgcagc gacgcgtcgc ggatgtcctt cgccaggccc gcctgccgcg ccgcgtccgc
    19921 gccgcccagg gccagcgtgt ccgccatgcc cacgagcgcg gccagcggcg tgcgcaggtc
    19981 gtgcgagatc gccgcgagca acgagttgcg cagccgctcc gactcgatgt ggacggtgct
    20041 ctcgcgcgcg acgtcgacgt agtggacgcg ctcgagcgat atcgcgacga gcgaggcgca
    20101 ggtctcgagc aggcgcatct gctccggccc ggcgaggcgc gacgggttgc gcggctcgat
    20161 cgcgagcacc ccgcgcaagc gcatcggcgc cttcagcggc aggtacagca cgggcgaggc
    20221 cggcagcgtg tgcgtgccct gccctgcggg ctctccatgg tcgtaggccc attgcgcgat
    20281 gccgcggtcg accttgcccg gcgcgctcgc cgagggcagc ggctcctgca cgcggtcgtc
    20341 caggtcggcc accagcagcg ccgagcgcgc ctcgaactcc gagtcgagga agcgcgccgc
    20401 gatctcggcg atctgctcga gcagcagcgc gccggacaga tcgcgcgaca tgcggtacag
    20461 cgcgcccacg cgttcctcgc gcagcatggc gacgcgcgcc tgccacttga ggacggccgt
    20521 gagctggccg atgaccaggc cgaccaccag catcacggag aaggtgacga ggtattgcac
    20581 gtccgtcacg ctgaaggaca ggcgcggcgg gacgtacagg aagtcgaacg cggccacgcc
    20641 gacgaaggcc gccagcaccg ccggcccgcg gccgtagcgc accgccgcga acacgaccgc
    20701 gagcaggaac accatcacga cgttggacag ctcgaacacc gcgctcagcg gcgccgcggc
    20761 cgccgccgcg atcgcgcaga cgagcaaggt cgccgcgtag gcccgccagg cggggcgcct
    20821 gccgtcctcg gtcggcccga tgcgcggacc ggcggccggc gtgggggcgt cgcc
//
LOCUS       DASVLN010000279         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_67865, whole genome shotgun sequence.
ACCESSION   DASVLN010000279 DASVLN010000000
VERSION     DASVLN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67865"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..>2235)
                     /locus_tag="VF453_06820"
     CDS             complement(33..>2235)
                     /locus_tag="VF453_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEX7687398.1"
                     /translation="AAEGRRQMSDDLREDPAPPGVTQRVGLGRRLPVILMTEASECGL
                     ACLAMIASWHGMRIDLPSLRLRFPISRKGGNFETLVRVASALGLDSRPLKLDMHNLSE
                     LQLPCILHWDMNHFVVLKSVGRRLVIHDPAVGVRSFSRDEFAQHFTGIAMELAPASGF
                     APRDERARFTLRGLMGRITGLKTGLAQILALALALEAVVIALPFYVQWVVDQALLAAD
                     RELLGVLALGFGLLVALQAGMGAVRAWWVATLSARLNFQWLGNVFGHLMRLPLEFFEK
                     RHLGHIVSVFGSVTVIQKTLTTGVVQAMVDGLMVAGTLAMMGLYSPRLLAISVVATAL
                     YGLLRWNVFRALREASAEEIVHAARQQTHFIESAAGIQSVRLFGRGGLRRAGWMNMLA
                     DQFNAGLRVQRVHVTHETAQTLLFGLERVLVVWLAARMVLDGAFTVGMLFAYLSYKEL
                     FSARVGALIDTASELAMLRLHGERVADVVLAPPEASEAPSPAEVDLSRRPARLELRRV
                     SYRYSPTEPWVLDGVDLVVEAGRSLAITGPSGCGKTTLVKVLLGLLPPTQGEVLFDGT
                     PIHRLGLDQYRALIGTVMQEDRLFSGSLADNIGFFDAEPDLERIEDCARLAAVDEEIR
                     EMPMGYHTLVGDVGIGISGGQKQRVLLARALYRRPRILVLDEATSHLDLHNERSVNEA
                     VRAMQLTRVIVAHRPETIAMADRVVEMADGRIVADSAPPAPAAPSLAREHA"
BASE COUNT          336 a          817 c          804 g          278 t
ORIGIN      
        1 cgccccccga gggggctggg ccgcgagcag cgtcaggcgt gctcgcgcgc gaggctggga
       61 gcggcgggcg ccggcggcgc gctgtcggca acgatccgcc catcggccat ctcgacgacg
      121 cggtcggcca tcgcgatggt ctcgggccgg tgggcgacga tcacgcgcgt cagctgcatc
      181 gcccgcaccg cctcgttgac gctgcgctcg ttgtggaggt cgaggtggct cgtcgcctcg
      241 tccagcacga ggatgcgcgg gcgccggtac aacgcccgcg cgagcagcac gcgctgcttc
      301 tgcccgccgg agatgccgat gcccacgtcg ccgaccagcg tgtggtagcc catgggcatc
      361 tcgcggatct cctcgtcgac ggcggccagg cgcgcgcagt cctcgatgcg ctcgaggtcg
      421 ggctcggcgt cgaagaagcc gatgttgtcc gcgagcgagc ccgagaacag ccggtcctcc
      481 tgcatcaccg tgccgatcag ggcgcggtac tggtcgagcc cgagccggtg gatcggcgtg
      541 ccgtcgaaga gcacctcgcc ctgggtgggc ggcagcaggc ccagcagcac cttcaccagc
      601 gtcgtcttgc cgcagcccga cggcccggtg atcgccaggc tgcgcccggc ctcgacgacg
      661 aggtcgacgc cgtcgagcac ccagggctcg gtcggcgagt agcggtagct cacgcgccgc
      721 agctccaggc gcgcgggacg ccgcgagagg tcgacctccg ccggcgacgg cgcctcgctc
      781 gcctcgggcg gcgccagcac gacgtcggcg acgcgctcgc cgtgcaggcg cagcatcgcg
      841 agctcggacg cggtgtcgat cagggcgccc acgcgggccg agaacagctc cttgtacgac
      901 aggtacgcga acagcatgcc caccgtgaac gcgccgtcca gcaccatgcg cgccgcgagc
      961 cagacgacca gcacgcgctc gagcccgaac agcagcgtct gcgcggtctc gtgcgtcacg
     1021 tgcacgcgct gcacgcgcag gccggcgttg aactggtcgg ccagcatgtt catccagccc
     1081 gcgcgccgca gcccgccgcg gccgaacagg cgcacgctct ggatgcccgc ggcgctctcg
     1141 atgaagtgcg tctgctggcg cgccgcgtgc acgatctcct cggcgctcgc ctcgcgcagc
     1201 gcgcggaaga cgttccagcg cagcaggccg tacagcgcgg tcgcgaccac cgagatcgcc
     1261 agcagccgcg ggctgtacag ccccatcatc gcgagcgtgc cggcgaccat cagcccgtcg
     1321 accatcgcct gcacgacgcc ggtggtcagc gtcttctgga tcaccgtgac cgagccgaac
     1381 acggagacga tgtggccgag gtggcgcttc tcgaagaact ccagcggcag gcgcatcagg
     1441 tggccgaaga cgttgcccag ccactggaag ttcagccgcg cgctgagcgt cgcgacccac
     1501 caggcgcgca ccgcgcccat cccggcctgc agcgccacca gcagcccgaa gccgagcgcg
     1561 agcacgccga gcagctcgcg gtcggcggcg agcagcgcct ggtcgacgac ccactgcacg
     1621 tagaacggca gcgcgatgac caccgcctcg agcgcgagcg ccagcgcgag gatctgcgcg
     1681 agcccggtct tcaggcccgt gatgcggccc atcaggccgc gcagcgtgaa gcgggcgcgc
     1741 tcgtcgcgcg gcgcgaagcc ggaggcgggc gcgagctcca tcgcgatgcc ggtgaagtgc
     1801 tgcgcgaact cgtcgcgcga gaagctgcgc acgccgacgg ccgggtcgtg gatcaccagg
     1861 cggcggccca ccgacttcag caccacgaag tggttcatgt cccagtgcag gatgcagggc
     1921 agctgcagct cggacaggtt gtgcatgtcg agcttgagcg gccggctgtc gaggccgagc
     1981 gccgaggcga cgcgcaccag cgtctcgaag ttgccgccct tgcgcgagat ggggaagcgc
     2041 aggcgcagcg acggcaggtc gatgcgcata ccgtgccagc tcgcgatcat cgccaggcac
     2101 gcgaggccgc actcgctcgc ctcggtcatg aggatcacgg gcagccggcg gccgaggccg
     2161 acgcgctggg tcacgcccgg cggcgccggg tcctcgcgca ggtcgtcgct catctggcgg
     2221 cgaccttcag ctgct
//
LOCUS       DASVLN010000280         7707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_389647, whole genome shotgun sequence.
ACCESSION   DASVLN010000280 DASVLN010000000
VERSION     DASVLN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389647"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1844)
                     /locus_tag="VF453_06825"
     CDS             complement(48..1844)
                     /locus_tag="VF453_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX7687399.1"
                     /translation="MKASGRAHRLTSLLAAATAALALALPAAAATREIVVEAPAPFKA
                     LLEKNLDVERAARLPEADSLDDNEWARLVAAVPAQGRALAETEGYFRADVTVAADPGD
                     PHRLTIRLVPNEPATVGRLTIEFDGDLARKAEAGDPQAQAIEAQVRAGWKLKPGAILR
                     NGDWTGDKAQALQALRAQGYAAAEWTATASQVDPSTNRARLFLVADSGPRFLAGNLVV
                     EGLERQPERTVRLLAGFDAGTPLTQQRLTDYQDRLEKTGLFDQVAVIYDPDPAQADHA
                     TITVHVHEQSLQQAQVAVGYSSTTGPRVTLEHTDRKPFGLAATTSNKLQWGRDLQQWD
                     FSLATHPAQDFHSWILGASIGRILTTGDDVRSGFIRFGRTQDSNPLDRMTFAEFERSL
                     ACTQYGCIDARALSLNQHGVWRRLDSVILPTKGYSINAQVGVGYAGGPDSKYGPYARL
                     YGRLTEYWPLGHDWYTQARVELGQIIVDPAVAMPDAEQWRAGGEDSVRGYAWRSLAPT
                     DAHGDIVGGNALITTSAEIAHPFTASLPSVWWATFVDAGRAAPRFNDLKMALGYGVGV
                     RWRSPVGPLKVDVSRGDEARRWRLDLSIGIAF"
     gene            complement(1841..3007)
                     /locus_tag="VF453_06830"
     CDS             complement(1841..3007)
                     /locus_tag="VF453_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687400.1"
                     /translation="MLIPFFYTLRDARLPVSVKEYLTLLEALRAGVIGPSIDDFYFLA
                     RTTLVKDEAHFDRFDKAFAAFFNGVAAVVNSKEIPEDWLRQLIERELTPEQKAAVAAM
                     GWDELMRTLQQRLEEQKGRHEGGSKWIGTGGTSPFGHGGFNPQGIRIGGAGKNRSAVK
                     VWEQRAYKDYDDSLELGTRNIKVALRRLRRFAREGAELELALDDTIHATAAAAGMLDI
                     KMQPERHNKVKVLLLMDVGGTMDEHIHRVEELFSAAKSEFKHLEFYYFHNCVYDFVWK
                     NNRRRFSEKHATWDVIRKYNKDYRLVFIGDATMSPYEILQPGGSVEYNNAEAGAEWLQ
                     RLANAFPKHCWINPEPQGVWQYRQSISLIQQLVGNRMFPLTLAGLEGAMRLLTK"
     gene            3241..4797
                     /locus_tag="VF453_06835"
     CDS             3241..4797
                     /locus_tag="VF453_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7687401.1"
                     /translation="MNFLSQWRIGRRLAVAFGVVVALTLASAGFTQLRLADVSRLSHE
                     LTTEQNERLALSYHWKNNIEVNSGRAVLMGMMTDQTFVATEMDKSKATSADTTAVQKR
                     FTELETTPQGKALIAAIAEVRARWLAQRDVMLKANDGTTSIAAAIAKGNAVKEFNGIV
                     DGYMAAANQLVTYETSRAKADGAAIDDALAMTRLVGGVCTLVCALFSIGLGLAITRSI
                     VRPVQAAQHAADRIAAGDLTGACRAEGTDEAAQLVKSLATMQDALKRIVREIRNSTEQ
                     IQVASTEVASGNQDLSARTEQTAGNLQQAASSMDELTSTMRHSADAATQAHGLAHSAS
                     EIAARGGQVVSQVVSTMDEINSSSKKISDIIGVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRSLAQRSAEAAKEIKSLIGTSVDKVETGARLVQDAGSTMQEIVQAVR
                     RVSEVLGEITTAASEQAGGIQLVNEAVNNVDQMTQQNAALVEQSAAAAESMKDQAGKL
                     AKAVEQFRTN"
     gene            complement(4939..5526)
                     /locus_tag="VF453_06840"
     CDS             complement(4939..5526)
                     /locus_tag="VF453_06840"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7687402.1"
                     /translation="MIDVRPVTLSDGRIRLEPMTLAHVDALKAAAADGELWNIRVTSV
                     PTPEDTQGYVERALAMKDRLPFVVVDLEAGGRVVGSTSYHDIVPAVDRVEIGWTWYAQ
                     SVQRSHVNTCAKLLLLVHAFETLGAQVVGWRTDNFNFASQRAIERLGAKRDGVIRHHA
                     LRRDGTVRDTVMYSVTRGEWPEVKAHLTYQANKPR"
     gene            complement(5537..6379)
                     /locus_tag="VF453_06845"
     CDS             complement(5537..6379)
                     /locus_tag="VF453_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7687403.1"
                     /translation="MKFEGSSNYVATPDLMLAVNAAMTLRRPLLVKGEPGTGKTMLAE
                     EAAAALGLPLLEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDTRVKDIHNYIVKGVLWQ
                     AFTSATPCALLIDEIDKADIEFPNDLLRELDRMEFHVYETKETIRAVQRPIVFITSNN
                     EKELPDAFLRRCFFHYIRFPDAPTMQRIVDVHFPGIKQELVAAALKSFYDVRNLPGLK
                     KKPSTSELLDWLKLLLAEDIPVAALQSKDEKVSIPPLIGALLKNEQDVTLFEKLIFMQ
                     KNNR"
     gene            6596..7237
                     /locus_tag="VF453_06850"
     CDS             6596..7237
                     /locus_tag="VF453_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7687404.1"
                     /translation="MTRTLTLLFALAAVFSQAHAQDAQRGATKVQMCIGCHGIVGYQA
                     DFPMVYKVPKIAGQDAKYIASALTEYRAGDRKFPTMRSIAASLSDQDIADIAAYYSKL
                     GHPDGPVPAKPEVPIPAELQSKVATCISCHGQNFSSPTDGTIPRLAGQYQDYLQQALT
                     SYQVEDNPHFGRKNPVMGAMAKPLKREDIEAITEYIASLPGELRTVPQEHFKK"
     gene            7421..>7707
                     /locus_tag="VF453_06855"
     CDS             7421..>7707
                     /locus_tag="VF453_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HEX7687405.1"
                     /translation="MLKKIRVDQVRLGMHLHSLEGAWMDHPFWKTRFVIRDAEQLKKL
                     QDSVIAEVWIDPEKGLDVADDTPALPAPMRSMAAPAPARPTPTIAEPYAPAT"
BASE COUNT         1141 a         2641 c         2732 g         1193 t
ORIGIN      
        1 ggcggcggtg ccttcgggtc gggtgtgcgg tcgtccacgt cgggccctca gaacgcgatg
       61 ccgatgctca ggtcgagccg ccagcggcgc gcctcgtcgc cgcgcgagac gtcgaccttc
      121 agcggcccga ccgggctgcg ccagcgcacc ccgacgccgt agcccagcgc catcttgagg
      181 tcgttgaagc gcggcgccgc gcggccggcg tcgacgaagg tcgcccacca caccgagggc
      241 agcgaggccg tgaacggatg ggcgatctcg gcgctggtcg tgatcaacgc gttgccgccc
      301 acgatgtcgc cgtgcgcgtc cgtcggcgcg agcgagcgcc acgcgtagcc gcgcaccgag
      361 tcctcgccgc ccgcgcgcca ctgctcggcg tccggcatcg cgacggcggg gtcgacgatg
      421 atctggccca gctcgacgcg cgcctgcgtg taccagtcgt ggcccagcgg ccagtactcg
      481 gtcaggcggc cgtacaggcg cgcgtagggg ccgtacttcg agtccggccc gcccgcgtag
      541 ccgaccccca cctgcgcgtt gatcgagtag cctttcgtcg gcaggatcac gctgtccagg
      601 cgtcgccaga cgccgtgctg gttgagcgac agcgcgcgcg cgtcgatgca gccgtactgc
      661 gtgcaggcga gcgagcgctc gaactccgcg aaggtcatgc ggtcgagcgg gttggagtcc
      721 tgcgtgcggc cgaagcggat gaagcccgag cgcacgtcgt cgccggtcgt caggatgcgg
      781 ccgatgctcg cgccgaggat ccagctgtgg aagtcctgcg ccggatgcgt cgcgagcgag
      841 aagtcccact gctgcaggtc gcggccccac tgcagcttgt tgctcgtcgt cgcggccagg
      901 ccgaagggct tgcggtcggt gtgctcgagg gtgacgcgcg ggccggtcgt cgagctgtag
      961 ccgacggcga cctgggcctg ctgcagcgat tgctcgtgca cgtgcacggt gatcgtcgcg
     1021 tggtcggcct gcgccgggtc ggggtcgtag atgacggcga cctggtcgaa caggccggtc
     1081 ttctccaggc gatcctggta gtcggtcagc cgctgctgcg tgagcggcgt gcccgcgtcg
     1141 aagccggcga gcagccgcac ggtgcgctcg ggctggcgct ccagaccctc gacgaccagg
     1201 ttgcccgcga ggaaacgcgg gccgctgtcg gcgaccagga acagccgcgc ccggttggtc
     1261 gacgggtcga cctggctcgc ggtggccgtc cactcggcgg ccgcgtagcc ctgcgcgcgc
     1321 agcgcctgca gggcctgcgc cttgtcgccg gtccagtcgc cgttgcgcag gatcgcgccc
     1381 ggcttgagct tccagcctgc gcgcacctgg gcctcgatcg cctgcgcctg cgggtcgccg
     1441 gcctcggcct tgcgcgcgag gtcgccgtcg aactcgatcg tcaggcggcc gaccgtcgcc
     1501 ggctcgttgg gcacgaggcg gatcgtgagg cggtgcgggt cgcccggatc ggccgcgacg
     1561 gtgacgtcgg cgcggaagta gccctcggtc tcggccagcg cgcggccttg cgccggcacg
     1621 gcggcgacca ggcgcgccca ttcgttgtcg tcgaggctgt cggcctcggg caggcgcgcg
     1681 gcgcgctcga cgtcgaggtt cttctcgagc agcgccttga acggcgccgg cgcctcgacg
     1741 acgatctcgc gcgtcgccgc ggccgccggc agggcgagcg cgagcgccgc cgtcgcggcg
     1801 gccagcaggg aagtcagtcg atgcgcgcgc ccgctcgcct tcatttcgtc aacaggcgca
     1861 tcgccccttc cagcccggcg agggtcagcg ggaacatgcg gttgccgacg agctgctgga
     1921 tcagcgagat gctctgccgg tactgccaga cgccttgcgg ctcgggattg atccagcagt
     1981 gcttgggaaa ggcgttggcc aggcgctgca gccactcggc gcccgcctcg gcgttgttgt
     2041 actcgacgct gccgcccggc tgcaggatct cgtagggact catcgtcgcg tcgccgatga
     2101 agacgaggcg gtagtccttg ttgtacttgc ggatgacgtc ccaggtcgcg tgcttctcgg
     2161 agaagcggcg ccggttgttc ttccacacga agtcgtagac gcagttgtgg aagtagtaga
     2221 actcgaggtg cttgaactcg ctcttggcgg ccgagaacag ctcctcgacg cggtggatgt
     2281 gctcgtccat cgtgccgccc acgtccatca gcagcagcac cttgaccttg ttgtggcgct
     2341 cgggctgcat cttgatgtcg agcatgcccg cggccgccgc ggtggcgtgg atggtgtcgt
     2401 ccagcgcgag ctcgagctcc gcgccctcgc gcgcgaagcg ccgcagccgg cgcagcgcga
     2461 ccttgatgtt gcgggtgccg agctcgagcg agtcgtcgta gtccttgtag gcacgctgtt
     2521 cccacacctt gaccgcgctg cggttcttgc ccgcgccgcc gatgcggatg ccctgcggat
     2581 tgaagccgcc gtggccgaag ggcgaggtgc cgccggtgcc gatccacttg ctgccgccct
     2641 cgtggcggcc cttctgctcc tcgaggcgct gctgcagcgt cctcatgagc tcgtcccagc
     2701 ccatcgcggc gacggccgcc ttctgctcgg gcgtgagctc gcgctcgatg agctggcgca
     2761 gccagtcctc ggggatctcc ttcgagttga cgaccgcggc cacgccgttg aagaacgccg
     2821 cgaaggcctt gtcgaagcgg tcgaagtgcg cctcgtcctt caccagcgtc gtgcgggcga
     2881 ggaagtagaa gtcgtcgatc gacgggccga tcaccccggc gcgcagcgcc tccagcaggg
     2941 tgaggtactc cttcacggag accggcagcc tcgcgtcgcg cagcgtgtag aagaagggga
     3001 tcagcatggc ggcgggacgg gggcgcgggc agctctgggg cgtggagcgt gaagagtatc
     3061 accgttgcaa ggccgcgcag ggcgcgggcc tcaagctccg gcgggaatgg tcgatgaccc
     3121 gggagatgca cggtttcggg gcgccttatc gggccttggc accgaggtgg acacgacaga
     3181 attttcctca gacacgcccc ctttcgggtg ggggctcgaa cgaacgcagg agacgtttcg
     3241 atgaacttcc tcagtcaatg gcgcatcggg cggcggctgg ccgtcgcctt cggcgtggtg
     3301 gtcgcgctca cgctggcctc ggccggcttc acccagctgc ggctggcgga cgtctcgcgg
     3361 ctgtcgcacg agctcacgac cgagcagaac gagcgcctcg cgctgtccta tcactggaag
     3421 aacaacatcg aggtcaactc cggccgcgcc gtgctgatgg gcatgatgac ggaccagacc
     3481 ttcgtcgcca cggagatgga caagtccaag gcgacgagcg cggacaccac cgcggtgcag
     3541 aagcgcttca ccgagctcga gacgacgccg cagggcaagg cgctgatcgc cgcgatcgcc
     3601 gaagtccgcg cccgctggct tgcgcagcgc gacgtcatgc tcaaggccaa cgacggcacg
     3661 acgtccatcg ccgcggccat cgccaagggc aacgccgtca aggaattcaa cggcatcgtc
     3721 gacggctaca tggcggcggc gaaccagctc gtcacctacg agacctcgcg cgccaaggcc
     3781 gatggcgccg cgatcgacga cgcgctcgcg atgacgcgcc tggtcggcgg cgtgtgcacg
     3841 ctggtgtgcg ccctgttctc gatcgggctg ggcctcgcga tcacccgcag catcgtgcgc
     3901 cccgtgcagg ccgcgcagca cgccgccgac cgcatcgccg cgggcgacct caccggcgca
     3961 tgccgcgccg agggcaccga cgaggccgcg cagctggtca agtcgctggc gacgatgcag
     4021 gacgcgctca agcgcatcgt gcgcgagatc cgcaattcga ccgagcagat ccaggtcgcg
     4081 agcaccgagg tggccagcgg caaccaggac ctgagcgcgc gcaccgagca gaccgccggc
     4141 aacctgcagc aggccgccag ctcgatggac gagctcacca gcaccatgcg ccacagcgcc
     4201 gacgccgcga cccaggccca tggcctcgcg cactcggcct ccgagatcgc cgcgcgcggc
     4261 ggccaggtgg tgtcgcaggt cgtctcgacg atggacgaga tcaactcgtc gtccaagaag
     4321 atctccgaca tcatcggcgt gatcgacggc atcgcgttcc agaccaacat cctggcgctc
     4381 aacgccgcgg tcgaggccgc gcgcgcgggc gagcagggcc gcggcttcgc ggtcgtcgcg
     4441 ggcgaggtgc gttcgctggc gcagcgctcg gccgaggcgg ccaaggagat caagtccctg
     4501 atcggcacca gcgtcgacaa ggtcgagacc ggcgcgcgcc tcgtgcagga cgcgggctcg
     4561 acgatgcagg agatcgtgca ggccgtgcgc cgcgtgagcg aggtgctggg cgagatcacc
     4621 accgccgcga gcgagcaggc cggcggcatc cagctcgtca acgaggccgt caacaacgtc
     4681 gaccagatga cgcagcagaa cgcggcgctc gtcgagcagt cggccgcggc cgccgagtcg
     4741 atgaaggacc aggccggcaa gctggccaag gcggtcgagc agttccgcac gaattgacgg
     4801 cccccacggt cgctggcgct ccctgccccc ccagggggcg tgcgcgtgca gaccggcgga
     4861 gccggatctg ccgcttcccc gtgtctcgct tgacgcgtcc gggcggcagc gccggtcgga
     4921 tgaaaacgag ccgcgcggtc agcgcggctt gttcgcttgg taggtcaggt gcgccttgac
     4981 ctcgggccat tcgccgcgcg tgacgctgta catcaccgtg tcgcgcacgg tgccgtcgcg
     5041 gcgcagggcg tggtggcgga tgacgccgtc gcgcttcgcg cccaggcgct cgatggcgcg
     5101 ctggcttgcg aagttgaagt tgtccgtgcg ccagcccacg acctgggcac ccagcgtctc
     5161 gaacgcgtgc accagcagca gcagcttcgc gcaggtgttg acgtgcgagc gctgcaccga
     5221 ctgcgcgtac cacgtccacc cgatctcgac gcggtcgacg gcaggcacga tgtcgtggta
     5281 gctcgtcgag ccgacgacgc ggccgccggc ctcgaggtcg accaccacga agggcaggcg
     5341 atccttcatc gcgagggcgc gctcgacgta gccctgcgtg tcctcgggcg tcggcaccga
     5401 ggtgacgcgg atgttccaca gctcgccgtc cgcggccgcg gccttcagcg cgtcgacgtg
     5461 cgcgagcgtc atcggctcga gccggatgcg gccgtcgctc agcgtgacgg gtcggacgtc
     5521 gatcatgccg ccgtcctcac cggttgttct tctgcatgaa gatcagcttc tcgaacaggg
     5581 tgacgtcctg ctcgttcttc agcagcgcgc cgatcagcgg cgggatcgag accttctcgt
     5641 ccttcgattg cagcgccgcg acggggatgt cctcggccag cagcagcttg agccagtcca
     5701 gcagctcgga ggtcgagggc ttcttcttca ggcccggcag gttgcgcacg tcgtagaagc
     5761 tcttgagcgc cgcggccacg agttcctgct tgatgcccgg gaagtgcacg tcgacgatgc
     5821 gctgcatggt cggcgcgtcg gggaaccgga tgtagtggaa gaagcagcgg cgcaggaagg
     5881 cgtcgggcag ctccttctcg ttgttcgacg tgatgaagac gatggggcgc tgcacggcgc
     5941 ggatcgtctc cttggtctcg tagacgtgga actccatgcg gtcgagctcg cgcagcaggt
     6001 cgttcgggaa ctcgatgtcg gccttgtcga tctcgtcgat caggagggcg cagggcgtcg
     6061 cgctcgtgaa ggcctgccac agcacgccct tgacgatgta gttgtggatg tccttcacgc
     6121 gcgtgtcgcc gagctggctg tcgcgcaggc ggctgacggc gtcgtactcg tacaggccct
     6181 gctgcgcctt ggtggtgctc ttgatgtgcc actcgagcag cggcaggccg agcgcggcgg
     6241 ccgcctcctc ggcgagcatc gtcttgcccg tgccgggctc gcccttgacc agcagcggcc
     6301 ggcgcagcgt catcgccgcg ttgacggcga gcatgaggtc gggagtcgcg acgtagttgc
     6361 tggagccttc gaacttcatg gaaatgcgtg tgcgatggga cggcggcgcg cgccgcggac
     6421 gggccggggg catggccggc gggaaggggg agggcgtcga tgcgggggcg ggttttgccg
     6481 gacggcgggc ccgttccagt ataaggtcac gtccctataa tccgtcgctg ccgggcaagg
     6541 ccgccggcga gccgggaatc cctccctcga ttctgagacc accgaagacg agacgatgac
     6601 gagaaccctg accttgctgt tcgccctcgc ggctgttttc tcccaggccc atgcgcagga
     6661 cgcgcagcgc ggcgcgacca aggtccagat gtgcatcggc tgccacggca tcgtcggcta
     6721 ccaggccgat ttcccgatgg tctacaaggt gcccaagatc gcgggccagg acgccaagta
     6781 catcgcctcc gcgctcaccg agtaccgagc cggcgaccgc aagttcccga cgatgcgcag
     6841 catcgcggcc tcgctgagcg accaggacat cgcggacatc gcggcctact actcgaagct
     6901 cggccatccc gacggtcccg tgccggccaa gcccgaggtg ccgatcccgg cggagctgca
     6961 gtccaaggtc gcgacctgca tctcctgcca cgggcagaac ttctcgtcgc ccactgacgg
     7021 caccatcccg cgcctggccg gccagtacca ggactacctg cagcaggcgc tgacctcgta
     7081 ccaggtcgag gacaaccccc acttcggccg caagaatccg gtgatgggcg cgatggccaa
     7141 gccgctcaag cgcgaggaca tcgaggcgat caccgagtac atcgcttcgc tgcccggcga
     7201 gctgcgcacg gtgccgcagg agcacttcaa gaagtgaccc gcgcgcgccg cgcgcggggc
     7261 gcggcatgac gaagcgccgc cggacccgcg tgtccggcgg cgcttgtcct tggggaatgt
     7321 caactcttca cggtcgcgtt cgcgcgcttg ccctacagtt ggcggccggc gtgccgaagt
     7381 tcctgctagc ccggccttcg ccggtgcttc cgcgccgccc atgctcaaga agatccgcgt
     7441 cgaccaggtc cgcctcggca tgcacctcca ttcgctcgaa ggcgcgtgga tggaccatcc
     7501 attctggaag acgcgcttcg tcatccgcga tgccgagcag ctgaagaagc tgcaggacag
     7561 cgtcatcgcc gaggtctgga tcgatccaga gaagggcctg gacgtcgccg acgacacgcc
     7621 cgcgctgccg gcgccgatgc gctccatggc ggcaccggcg cctgcccggc cgacgccgac
     7681 gatcgccgag ccgtatgcgc cggccac
//
LOCUS       DASVLN010000281        11559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_445188, whole genome shotgun sequence.
ACCESSION   DASVLN010000281 DASVLN010000000
VERSION     DASVLN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445188"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1160
                     /locus_tag="VF453_06860"
     CDS             87..1160
                     /locus_tag="VF453_06860"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEX7687406.1"
                     /translation="MTRQHLPAGLALREYGHLPDGRVVHEATLSDGAGLVLRAITLGG
                     IVTRLEVPDREGEVANVVAGFADLADYVERNPHFGVIVGRYANRIAGAAMEIDGQRFE
                     LDRNDGPNTLHGGARGWGKRLWEIESAQSQGSPSLVLRYVSADGEEGFPGRVEARVTY
                     ALAGDRTWVVDYEARTDKPTVVNMSHHDYFNLAGGGSAMGHVLTLPASRFTAIDRTLI
                     PTGIADVEGTPLDFRAATPIGARIGQPHEQLALAGGYDHNWILDEPANGRPRFAARLE
                     DPASGRVLLLETSEPALQFYSGNFLDGSLRGPEGQAYEHRSAVCLEPQHNPDSPHHPD
                     WPSTILRPGEVYRSRSVYRFEAN"
     gene            complement(1157..1564)
                     /locus_tag="VF453_06865"
     CDS             complement(1157..1564)
                     /locus_tag="VF453_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7687407.1"
                     /translation="MTTVKLWTDDEAAADPRVAAVFADIRATRRTDFVNNIWRALANQ
                     PALLERTWQGVKDVMVAPGALDPLVKEMVYIAVSAANNCGYCVHSHTAAARAKGMTDA
                     QHAELMAIVALASSTNAIASAMQFPTDDRFLVA"
     gene            1784..3724
                     /gene="pepF"
                     /locus_tag="VF453_06870"
     CDS             1784..3724
                     /gene="pepF"
                     /locus_tag="VF453_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX7687408.1"
                     /translation="MKRTVLALAIALGYLGAAQAANAPAAPAAPASSVAAASNPPETW
                     NLSQLYPTDAAWETSRAKLEADLPKLKALQGTLGANAKSLLAGLDQISAANRELARLF
                     TYAQLKGDENTKVTANEARRGQAATLATQLGTALSWEDSEIAALGKDRIEQYIAEEPG
                     LAKHAYHLRSILRQAAHVLPADQEAIVAAAQDPLSRTSRIYDILTNADLPWPKITVHG
                     KTVTLDQETYVTLRNDADPKVREQVFKAFWPVFKAYTRTIGAIYETHLRGQVFDAREH
                     KYDSVLAAKTSDLNTPETVYRTLVAEANAGLPVLQRYLKMRGKLLGLKEQKYSDVYAT
                     LAKAPRTYTLGEAEQLVLKGVAPLGDDYVKALGQHFQEGWMDAVPRLGKRSGAYMEPA
                     AYDVHPYVLLSFNGTYDSVSTIAHEWGHAMHSVLAEANQPYETADYGIFVAEIPSTTN
                     EMLLADYVSAHAKTKAEKIYALTQQLEGMRTAFFRQAMFAEFELKAHEAAEKDQPLTG
                     DDFSRIYLDLLKRYYGDAQGVMKIEDLYGAEWAYIPHFYTDYYVYQYATCISAAAYFA
                     EGIEKGDTALRTRYLDMLKAGGSDDPYLVVKKAGLDMATPAPYRALVQRMSHLLDDLD
                     ALTAAPPARATAPAKAAASAAR"
     gene            complement(4273..4797)
                     /locus_tag="VF453_06875"
     CDS             complement(4273..4797)
                     /locus_tag="VF453_06875"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HEX7687409.1"
                     /translation="MKLKAILTSLALAAIGTGASAANVVDWGVLGPVTDVAYVTYHTD
                     GPIDDVYTFSIGNWSDVPTYAEEFEARSVSMENAQFTLYSGTYGDPGATMVGTPFPFT
                     NTATLTTYTSLASGNYYFEITGTSAMAGSAYDFEASANESGPPSDVPEPTNMALLLAG
                     VGLIGFVARRRGAR"
     gene            5764..7653
                     /locus_tag="VF453_06880"
     CDS             5764..7653
                     /locus_tag="VF453_06880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX7687410.1"
                     /translation="MNRNRKAPTATPALALAALVVASLTACGGGGSAMTEQADAADST
                     TAATAAASATAESDLDAQLAQASATPTYHMAPVVLPAPDESDVGGTNASARVAPQRFA
                     IDPAVADLSTRNLTPDVLAQHLADARMHPASAGSAATTKSLTSTVFTPAQIRAAYGLP
                     DLPAVTTSLTAAQAATLGAGQTIYIIDAYHDASALTDLNVFSTKFGLPTCTQTAIATT
                     AAMPLAAAPSRCTLSVVEATSTGTMTSTVPSYNGTWAPESKLDVQWAHAIAPLARIVL
                     IETPNSMSNNILGAITLANKMGPGVVSMSFAFPDPGWAASVDSYFTKTAGMTFVAATG
                     DVGGQVAWPAVSPHVLAVGGTGMNWTPTSGRYEQAWASGGGGVSAYEAMPAYQSAVTV
                     GGARPSHRAVADVAFNASPTTGQYVSLTLPGHVQAWSAYGGTSIGTPQWAGIVAVADA
                     IRANAGKGMLGDIHSALYSSIGAVPGNYAAAFDDIVDGTNGTCSICKATAGFDTATGW
                     GSPNVTALLPLLTGTSASVAASPSPAPAPTPAPTTNHAPAISSQTVNVKYSKAFSYQL
                     QGRDADGDALTYTISGAPAGVSLSSSGMLTWSSPSTKGRFGTTVTVRDSHGASSTGVI
                     ILLVA"
     gene            complement(7946..9127)
                     /locus_tag="VF453_06885"
     CDS             complement(7946..9127)
                     /locus_tag="VF453_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7687411.1"
                     /translation="MTTSTRRSLLQAAGWTTLALAAALAGCGKKDETPAPAAVPAADA
                     GSAASAAAAPAPAASGPLKIAFAYVGPVGDGGWSYGHDLGRKALEAALGDKVKTTFVE
                     KVPEGADAERVFRDLVAQGNTLVFGTSFGYMEPMLKVAADNKGVKFEHATGYKTAENL
                     RTYDARTYEGAYMAGVVAGGMTKTGTLGVVGSIPIPEVIRNIDSFTLGAQSVNPKVKV
                     RVVWVNEWFNPPKETEGAQSLIDGGADVLFQNTDSSAVLQTAEKNGKYGLGWDSDMKS
                     YGPKAHLASAVENWGPYYIKAANDVLNGTWTTGKSWWGVKEGAIDLVSISDKVPADLK
                     AKVDQVKSGLKDGSFAIWKGPIASNDGKDVLKAGETADDKFLGGIKFYVKGIEGQVPG
                     N"
     gene            complement(9177..10097)
                     /locus_tag="VF453_06890"
     CDS             complement(9177..10097)
                     /locus_tag="VF453_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7687412.1"
                     /translation="MNEWALLVAATLNAGTVLAIAGLGLLVNERAGVVNLGAEGIMLV
                     AAIAGFATAVHTGSDTLAFGAGALAGAAMAAAFGLLVIWLNTNQVATGLALSLFGSGF
                     SAFVGIRYTQERLGPRPHFAIPGLADLPFAGPALFRQHPMVYFAIALAVGLSWFLYRS
                     RAGLVLRAVGESPESAHALGYPVRRIRLAAVMAGGALCGLAGAYVSVIYTPLWVEGMI
                     AGKGWIALALTTFATWRPARVLLGAWLFGGVTMLQFHLQAEGVQVPSQLLTMLPYLAT
                     IAVLALISRNPAWIRANMPASLGKPFFPGG"
     gene            complement(10094..11176)
                     /locus_tag="VF453_06895"
     CDS             complement(10094..11176)
                     /locus_tag="VF453_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7687413.1"
                     /translation="MLRLEARPAPSRAMSWASPAIALAVTTLAGLALFALLGKDPLQG
                     LGVFFVEPFRGANALSELAVKATPLMLIGLGLAISYRADVWNIGAEGQFVLGAIAASG
                     LAIHVGDGASHWVVVPILLAGVVGGMAWAAIVAFLKDRANTNEILVSLMLVYVAEQLL
                     GYLVYGPWKDPQGYNFPQTVTFAAATQVPRLVTGLRVNVGFLLALAAVAASWVWLFRL
                     RGGFRLQVGGLAPAAARYAGFSSRHALWVALLASGGLAGLAGALEVAGPLGQLTPYVP
                     AGYGFAAIIVAYVGRLHPVGVLFSALLMSLFYIGGELAQSRLGCPKSLTGVFQGVLLF
                     SLLASDTLIHFRVRRAARHAAGGAAR"
     gene            complement(11163..>11559)
                     /locus_tag="VF453_06900"
     CDS             complement(11163..>11559)
                     /locus_tag="VF453_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7687414.1"
                     /translation="ARFGVKAGGPDAPARSLSGGNLQKFLVGREIDARPTLLVVAQPT
                     WGVDVGAAAQIRGELLALRDAGCAVLVVSEDLDELLEISDRLVVIARGRVSPSLPVAE
                     ASPARIGEWMSGLWPAASTDSTEAAHAAA"
BASE COUNT         1830 a         4274 c         3863 g         1592 t
ORIGIN      
        1 cgcgcgcagg ccgaggcgcg cgcctcgcac ggcctccatc ccgccatcga cagccaccac
       61 gaacgactgc acagccggag cttcacatga cacgccaaca cctccccgcc ggcctcgcgc
      121 tgcgcgagta cggccacctg cccgacggac gcgtcgtgca cgaggccacc ctgtccgacg
      181 gcgcgggcct cgtgctgcgc gcgatcacgc tgggcggcat cgtcacgcgc ctggaagtgc
      241 ccgaccgcga aggcgaggtc gccaacgtcg tcgccggctt cgcggatctc gcggactacg
      301 tcgagcgcaa cccgcacttc ggcgtgatcg tcggccgcta cgccaaccgc atcgcgggcg
      361 cggcgatgga gatcgacggc cagcgcttcg agctcgatcg caacgacggc ccgaacacgc
      421 tgcacggcgg ggcgcgcggc tggggcaagc gcctgtggga gatcgagtcc gcgcagtcgc
      481 agggcagtcc gtcgctcgtg ctgcgctacg tgagcgccga cggcgaagaa ggattccccg
      541 gccgcgtcga ggcgcgcgtg acctatgcgc tcgcgggtga tcgcacctgg gtggtcgact
      601 acgaggcccg cacggacaag cccacggtgg tcaacatgag ccaccacgac tacttcaacc
      661 tcgccggcgg cggcagcgcg atgggccacg tgctgacgct gcccgcgagc cgcttcacgg
      721 cgatcgacag gacgctgatc ccgaccggca tcgcggacgt cgagggcacg ccgctcgact
      781 tccgcgccgc gacgccgatc ggcgcgcgca tcggccagcc gcacgagcaa ctggcgctcg
      841 cgggcggata cgaccacaac tggatcctcg acgagcccgc gaacggccgg ccgcgcttcg
      901 cggcgcggct cgaggatccc gcctcgggcc gcgtgctgct gctcgagacg agcgagccgg
      961 cattgcagtt ctattcgggc aacttcctcg acggctcgct gcgcggcccc gagggccagg
     1021 cctacgagca ccgttcggcc gtctgcctcg agccgcagca caaccccgac tcgccccatc
     1081 accccgactg gcccagcacg atcctgcggc cgggcgaggt gtaccgcagc cgttcggtct
     1141 accgcttcga ggctaactag gcgacgagga acctgtcgtc ggtcgggaac tgcatcgccg
     1201 acgcgatcgc gttcgtgctc gacgccagcg cgacgatggc catcagctcc gcgtgctggg
     1261 cgtcggtcat gcccttggcg cgggcagcgg cggtgtgcga gtgcacgcag tagccgcagt
     1321 tgttggcggc ggagaccgcg atgtagacca tctccttgac cagcgggtcc agggcgccgg
     1381 gcgcgaccat cacgtccttc acgccctgcc aggtgcgctc cagcagtgcg ggctggttgg
     1441 cgagggcgcg ccagatgttg ttgacgaagt ccgtgcggcg agtcgcgcgg atgtcggcga
     1501 agacggcggc cacgcgcggg tcggcggcgg cctcgtcgtc ggtccatagc ttgacggtgg
     1561 tcatgcggcg tttctccatc gagtgcgtgt gccgcggact gtagcgagct cgcgcgcgca
     1621 cgtcccgtcg tccaggcaaa cccgtacgcg tcagcccgaa caagggtttg tcccggcgcc
     1681 attcggcccc gcgattcgcc atttcgtcgc gcccgcgcca tccagtcccc taggcaagcg
     1741 gtatcgtcgc ggctttgccg atcacgcgac attgcccacg accatgaaac gcaccgtcct
     1801 cgccctcgcc atcgccctcg gatacctcgg ggccgcccag gccgcgaacg cgcccgccgc
     1861 accggccgcc ccggcctcgt ccgtcgcggc ggcgtcgaac ccgcccgaga cctggaacct
     1921 gtcgcagctc tatccgaccg acgccgcctg ggagacctcg cgcgccaagc tcgaggccga
     1981 cctgcccaag ctcaaggcgc tgcagggcac gctgggcgcg aacgcgaaga gcctgctggc
     2041 cggcctcgac cagatctccg ccgccaaccg cgagctcgcc cgcctgttca cgtatgcgca
     2101 gctcaagggc gacgagaaca ccaaggtcac cgccaacgaa gcccgccgcg gccaggccgc
     2161 cacgctcgcg acgcagctgg gcacggcgct gtcctgggag gactccgaga tcgccgccct
     2221 cggcaaggac cggatcgagc agtacatcgc cgaggagccg ggcctggcca agcacgccta
     2281 ccacctgcgc tcgatcctgc gccaggccgc gcacgtgctg ccggccgacc aggaggccat
     2341 cgtggcggcg gcgcaggatc cgctctcgcg caccagccgc atctacgaca tcctgacgaa
     2401 cgccgacctg ccctggccga agatcaccgt ccacggcaag acggtgacgc tcgaccagga
     2461 gacctacgtc acgctgcgca acgacgcgga cccgaaggtg cgcgagcagg tcttcaaggc
     2521 cttctggccc gtgttcaagg cctacacccg caccatcggc gcgatctacg agacccacct
     2581 gcgcggccag gtcttcgacg cgcgcgagca caagtacgac tccgtgctcg cggccaagac
     2641 gagcgacctc aacacgcccg agaccgtcta ccgcacgctc gtggccgagg ccaacgccgg
     2701 cctgccggtg ctgcagcgct acctgaagat gcgcggcaag ctgctcgggc tgaaggagca
     2761 gaagtactcc gacgtctatg ccacgctcgc caaggcaccc cgcacctaca cgctgggcga
     2821 ggccgagcag ctggtgctga agggcgtcgc gccgctgggc gacgactacg tcaaggcgct
     2881 gggccagcac ttccaggaag gctggatgga cgccgtgccg cgcctgggca agcgctcggg
     2941 cgcctacatg gagccggccg cgtacgacgt gcacccgtac gtgctcctga gcttcaacgg
     3001 cacctatgac agcgtctcga cgatcgccca cgagtggggc cacgcgatgc actcggtgct
     3061 cgccgaggcc aaccagccct acgagacggc cgactacggc atcttcgtcg ccgagatccc
     3121 gtcgacgacc aacgagatgc tgctggccga ctacgtctcg gcgcatgcga agaccaaggc
     3181 cgagaagatc tatgcgctca cgcagcagct cgagggcatg cgcacggcgt tcttccgcca
     3241 ggccatgttc gccgagttcg agctgaaggc gcacgaggcg gccgagaagg accagccgct
     3301 gaccggggac gacttctcca ggatctacct cgacctgctc aagcgctact acggcgacgc
     3361 gcagggcgtg atgaagatcg aggacctgta cggcgccgag tgggcctaca tcccgcactt
     3421 ctacaccgac tactacgtgt accagtacgc gacctgcatc agcgccgccg cctacttcgc
     3481 cgagggcatc gagaagggcg acaccgcgct gcgcacgcgc tacctcgaca tgctcaaggc
     3541 aggcggctcg gacgatccgt acctggtcgt caagaaggcg ggcctggaca tggccacgcc
     3601 ggcgccgtac cgcgcgctgg tgcagcgcat gtcccacctg ctggacgacc tcgacgcgct
     3661 gaccgcggcc ccgcccgcca gggccacggc tccggccaag gcggccgcct ccgccgcgag
     3721 gtgacccgcc cgccggcgtc cggctcgacg ccgtcgccgg cgaccccggc ccatgggcgc
     3781 ccgctccggc gggcgtcttc gtttccggca ttcgcggcgc ggagtttccc aggccgcctt
     3841 gcgccggtta tctgcaggcg cccggataac cggatcgacc gtcgccggaa accacgcaac
     3901 ggttaccttt cgaatcgaat tccccggaac cggaatattc cccgccgaca gtttgtcgag
     3961 gcaatcgatg gcttcgcgtg ctcgccgggc gacgcgtgcc cgcgcgcccc ggcgggacat
     4021 ggccgagcag ctccgcgcgt ttcggccgcg accgcgactc gcggcgagcc gccgcggccg
     4081 gaaaaattaa cggtcgtgac gatcgagccg gcgtaaaggc tactcgacca catgacgcgc
     4141 cgtcccggcg ccgcagcgcg ccaggcgcct gcgtggagga gacccaccat cgcggcgggc
     4201 aagaaaaagg gcagcgcgcc agccgctgcc cttcgtaccg cccgcggcgc ccccgcgcgc
     4261 cgcggccgtt catcaacgcg cgccgcgacg acgcgccacg aagccgatca gcccgacgcc
     4321 tgcgagcagc agggccatgt tggtcggttc cgggacgtcc gacggcgggc ccgactcgtt
     4381 ggccgaggcc tcgaagtcgt atgcggagcc ggccatcgcc gacgtgcccg tgatctcgaa
     4441 gtagtagttg ccactggcca gcgacgtgta ggtggtcagc gtggcggtgt tggtgaacgg
     4501 gaagggcgtg cccaccatcg tcgcgccagg gtcgccgtag gtgcccgaat agagcgtgaa
     4561 ctgcgcgttc tccatcgaga cggaccgcgc ttcgaactct tcggcgtagg tggggacgtc
     4621 cgaccagttg ccgatgctga acgtgtagac gtcgtcgatc gggccgtcgg tgtggtacgt
     4681 cacgtacgcc acgtcggtga ccgggccgag cacgccccag tcgacgacgt tggcggcgga
     4741 agcgccggtg ccgatggcgg cgagcgcgag ggaagtcaga atggctttca gtttcacttg
     4801 ttggtcctcc tagcagtggc cttttgtgga gacccaggcc gcaaaggtct gggtaacgtt
     4861 ttgcaaccac tgttcccgca cgggcccgtc gcacctcggg aagaaccctt gaccgctttt
     4921 ggggaggccg tcggggcgtc ccagcgcgct gcagagcggt gcgtccggcg catcgatcga
     4981 tatcccttcg cggttccccg tcaattcgca agggcgccgc gatattcgcc aggcgctgcg
     5041 cgcccggaat actcgacctt tgatcgccgc ggggtttcat gaaacccgaa atccatgccg
     5101 tcggaggccg attttcctcc cgacaatatt cccagcccat ccgcacggag cggcatggcc
     5161 ggtgacgatc ccgctcggcc gttgttgccg ccgggcacct acctcaactc gaccatccga
     5221 ggcaagtcgg gaaaaatttc gtgcccggtc gaaaggggtc gaattgcaac atgagcggat
     5281 cagcatccgg ctcgacagtg agcatcggtg gagtcgacga tgtcgcagac cacgccgtaa
     5341 agggagcttc agaagagact gcagttttcg tcatggcaaa ctctgctcat caataaaacg
     5401 cgactcgcga attactgccg agctgcattc cgtttcattt caaccgattg cccggtagat
     5461 gctgcggtaa tattcgctcg ccgtcgaaga acggcaccca gttgtccggt gtcatcgggc
     5521 cctgtccggc ctcgaccctg acgcactcgc tgctctgtcg tgatttcctg actcatcgcc
     5581 tccgggctgc ggtcgaagca cacaacagcg ccttgcagta ccagtctgcc tagcagcctg
     5641 gcactgcatg tccacacccg gcgggtccgg cgtgatggcg tgctggtcgc gatgcaccga
     5701 gcgtcgggat gggcgtgcct ttgcggatcc gtcgatgact tggcttcatc gaaggattcc
     5761 gttttgaacc gcaatcgcaa ggccccgacc gccacccccg ccctggccct cgccgccctc
     5821 gtcgttgcca gcctcaccgc ctgcggtggc ggcgggtcgg ccatgaccga gcaggccgac
     5881 gccgccgatt cgacgaccgc ggccaccgcc gccgcttcgg ccaccgccga gagcgacctc
     5941 gacgcccagc tggcccaggc ttccgccacg ccgacgtacc acatggcgcc ggtggtgctg
     6001 cccgcgcccg acgagagcga cgtcggcggc acgaacgcct cggcgcgcgt ggccccgcag
     6061 cgcttcgcga tcgaccccgc cgtcgcggac ctgtccacgc gcaacctgac gcccgacgtg
     6121 ctcgcccagc acctggccga cgcgcgcatg caccccgcct cggccggctc cgcggccacc
     6181 acgaagtcgc tgacctcgac cgtcttcacg ccggcccaga ttcgcgccgc gtacggcctg
     6241 cccgacctgc ccgccgtcac gacctcgctg accgccgcgc aggccgccac cctcggcgcc
     6301 ggccagacca tctacatcat cgacgcctac cacgacgcga gcgcgctgac cgacctgaac
     6361 gtcttctcga cgaagttcgg cctgccgacc tgcacgcaga cggcgatcgc cacgaccgcg
     6421 gcgatgccgc tggccgccgc gccgtcccgg tgcacgctgt cggtggtcga ggcgacctcg
     6481 accggcacga tgaccagcac cgtgccgagc tacaacggca cctgggcgcc ggagagcaag
     6541 ctcgacgtgc aatgggcgca cgcgatcgcg ccgctcgcgc gcatcgtgct gatcgagacc
     6601 ccgaactcga tgagcaacaa catcctcggc gccatcacgc tggccaacaa gatgggcccg
     6661 ggcgtcgtct cgatgagctt cgccttcccc gacccgggct gggcggcgag cgtcgactcc
     6721 tacttcacca agaccgctgg catgacgttc gtcgcggcga ccggcgacgt cggcggccag
     6781 gtggcctggc cggccgtctc gccccacgtg ctcgccgtcg gcggcacggg catgaactgg
     6841 acgccgacca gcggccgcta cgagcaggcc tgggccagcg gcggcggcgg cgtgagcgcc
     6901 tacgaggcga tgccggcgta ccagtcggcc gtcacggtcg ggggcgcgcg gccttcgcac
     6961 cgcgccgtcg cggacgtcgc gttcaacgcg agcccgacga ccggccagta cgtctcgctg
     7021 acgctgcccg gccacgtgca ggcgtggagc gcttacggcg gcaccagcat cggcacgccg
     7081 cagtgggcgg gcatcgtcgc cgtcgccgac gcgatccgcg ccaatgccgg caagggcatg
     7141 ctcggcgaca tccacagcgc gctgtacagc tcgatcggcg ccgtcccggg caactacgcc
     7201 gctgcgttcg acgacatcgt cgacggcacc aacggcacct gctcgatctg caaggccacc
     7261 gccggcttcg acaccgcgac cggctggggc tcgcccaacg tcaccgccct gttgcccctg
     7321 ctgaccggca cctccgcctc ggtcgccgcg tcgccgtcgc cggcaccggc cccgacgccc
     7381 gccccgacga ccaaccacgc gccggcgatc agcagccaga ccgtgaacgt caagtacagc
     7441 aaggcgttct cgtaccagct gcagggccgc gacgccgacg gcgacgcgct gacctacacc
     7501 atctcgggcg ccccggccgg cgtctcgctg tcgagctcgg gcatgctcac ctggtcgagc
     7561 ccgtcgacga agggccgctt cggcaccacg gtcaccgtgc gcgacagcca cggcgccagc
     7621 agcaccggcg tcatcatcct cctggtcgcc tgagcgtcct cgacctcgcc cacgacgccc
     7681 gcctcgcgcg ggcgtttttc atggcgcgcc ggccgggcgc gacgcgccgt ccgcgcgccg
     7741 aggaaggctg cgtcccagct catgcgcgca agcggatgtc gcatatcgat ggcggcgtat
     7801 ggcccggacg gatccgcgtg ggcgaagatg tccgctctcg ctgtcatcac gccccgcacc
     7861 cagggatttc caacgatccg tggcgtcacg gccgcgaccg cggcccgccc ggtccatgtc
     7921 gaccggcgcg gggcgcgcgt cccggtcagt tgccaggcac ctggccctcg atgcccttga
     7981 cgtagaactt gatgccgccg aggaacttgt cgtccgcggt ctcgccggcc ttgagcacgt
     8041 ccttgccgtc gttcgaggcg atcggcccct tccagatcgc gaagctgccg tccttcaggc
     8101 ccgacttgac ctggtcgacc ttggccttga ggtcggcggg caccttgtcg gagatggaca
     8161 ccaggtcgat cgcgccttcc ttgacgcccc accacgactt gccggtcgtc caggtgccgt
     8221 tgaggacgtc gttggccgcc ttgatgtagt acggccccca gttctcgacc gccgaggcga
     8281 gatgcgcctt gggaccatag gacttcatgt cggaatccca gccgaggccg tacttgccgt
     8341 tcttctcggc cgtctgcaga accgcgctgg agtccgtgtt ctggaacagc acgtccgcgc
     8401 caccgtcgat cagcgactgc gcgccctcgg tctccttggg cgggttgaac cactcgttga
     8461 cccacaccac gcgcaccttg accttcggat tcacgctctg cgcgccgagg gtgaagctgt
     8521 cgatgttgcg gatcacctcg gggatgggga tcgagccgac cacgcccagc gtgcccgtct
     8581 tcgtcatgcc gcccgcgacg acgcccgcca tgtacgcgcc ctcgtaggtg cgggcgtcgt
     8641 aggtgcgcag gttctcggcg gtcttgtagc cggtggcgtg ctcgaacttg acgcccttgt
     8701 tgtcggcggc gaccttgagc atcggctcca tgtagccgaa gctggtcccg aagacgagcg
     8761 tgttgccctg cgccacgagg tcgcggaaga cgcgctcggc gtcggcgccc tcgggcacct
     8821 tctcgacgaa ggtcgtcttg accttgtcgc ccagcgccgc ctcgagcgcc ttgcgaccga
     8881 ggtcgtgccc gtagctccag ccgccgtcgc ccacggggcc gacgtaggcg aaggcgatct
     8941 tcagcggccc ggaggccgcc ggcgcaggcg ccgcggcggc ggatgcggcc gagccggcgt
     9001 cggccgccgg taccgccgcg ggcgcgggcg tctcgtcctt cttgccgcag ccggccagcg
     9061 ccgcggcgag tgccagggtc gtccagcccg cggcctggag cagcgaacgc cgggtgcttg
     9121 tcgtcatggg gaatatcctc ggaggttgtc gtgcgtggga tgggtgaccc gggctctcaa
     9181 ccgcccggga agaacggctt gccgagcgag gccggcatgt tcgcgcggat ccaggccgga
     9241 ttgcgggaga tcagcgccag caccgcgatg gtggcgaggt agggcagcat cgtcagcagc
     9301 tggctcggca cctgcacgcc ctcggcctgg agatggaact gcagcatcgt cacgccgccg
     9361 aacagccagg cgccgagcag cacgcgcgcc ggccgccagg tggcgaaggt ggtcagggcg
     9421 agcgcgatcc agcccttgcc cgcgatcatg ccctcgaccc acagcggggt gtagatgacc
     9481 gacacgtacg cgcccgccag cccgcacagc gcgccgccgg ccatcacggc agcgagccgg
     9541 atccgccgca ccggatagcc gagcgcgtgc gcggactccg gcgactcgcc caccgcgcgc
     9601 agcacgaggc cggcgcggga tcggtacagg aaccacgaca gcccgaccgc cagcgcgatc
     9661 gcgaagtaga ccatcggatg ctggcggaac agcgcggggc cggcgaaggg gagatcggcg
     9721 aggcccggga tcgcgaagtg cggccgcgga ccgaggcgct cctgcgtgta gcggatgccg
     9781 acgaaggccg agaagccgct gccgaacagg ctcagcgcga ggccggtggc gacctgattg
     9841 gtgttcagcc agatcacgag caggccgaag gccgcggcca tcgcggcgcc cgccagcgcg
     9901 ccggcgccga acgcgagcgt gtcgctgccg gtgtggacgg cggtcgcgaa gccggcgatc
     9961 gcggcgacca gcatgatgcc ctcggcgccg aggttgacga cgccggcacg ctcgttgacc
    10021 agcagcccca gtcccgcgat cgcgagcacg gtgccggcgt tgagcgtcgc ggcgacgagc
    10081 agtgcccatt cgttcatcgc gcggcacccc cggcggcatg gcgcgcggcg cggcgcacgc
    10141 ggaagtggat cagcgtgtcg ctcgccagca gcgagaacag cagcacgccc tggaagacgc
    10201 cggtgagcga cttcggacag cccagccgcg actgcgcgag ctcgccgccg atgtagaaca
    10261 ggctcatgag cagcgccgag aacagcaccc cgaccggatg cagccgcccg acgtaggcga
    10321 cgatgatggc cgcgaagccg tagccggccg gcacgtaggg cgtgagctgg ccgagcggcc
    10381 cggcgacctc gagcgccccc gccaggccgg ccaggccgcc cgacgcgagc agcgcgaccc
    10441 acagcgcatg ccgcgaggag aacccggcat agcgcgccgc cgccggcgcc agcccgccga
    10501 cctgcagccg gaagccgccg cgcagccgga acagccacac ccagctcgcc gcgaccgcgg
    10561 ccagcgcaag gaggaagccg acgttcacgc gcaggccggt gaccagccgc ggcacctgcg
    10621 tcgccgcggc gaaggtgacc gtctgcggga agttgtagcc ctgcggatcc ttccacggcc
    10681 cgtagacgag gtagccgagc agctgctccg cgacgtagac cagcatcagg ctcacgagga
    10741 tctcgttggt gttggcgcga tccttcagga aggcgacgat cgccgcccag gccattccgc
    10801 cgacgacgcc cgcgagcagg atgggcacga cgacccagtg gctcgcgccg tcgccgacat
    10861 ggatcgccag ccccgacgcc gcgatcgcgc ccagcacgaa ctggccctcg gcgccgatgt
    10921 tccagacgtc ggcgcggtac gagatcgcca ggcccaggcc gatcagcatc agcggcgtcg
    10981 ccttgaccgc gagctcggac agcgcgttcg cgccgcggaa gggctcgacg aagaacacgc
    11041 ccaggccctg cagcggatcc ttgcccagca gcgcgaacag cgccagcccg gcgagcgtcg
    11101 tgaccgccag cgcgatcgcg ggcgacgccc aggacatcgc gcggctgggc gcgggacggg
    11161 cctcaagccg cagcatgcgc cgcctccgtc gaatccgtcg acgccgccgg ccacagtccc
    11221 gacatccact cgccgatgcg cgccggcgag gcctcggcca ccggcagcga cggcgacacg
    11281 cggccgcgcg cgatcacgac caggcggtcg ctgatctcga gcagctcgtc cagatcctcg
    11341 ctgaccacca gcaccgcgca gccggcatcc cgcagcgcga gcagctcgcc gcggatctgc
    11401 gccgccgcgc cgacatcgac gccccaggtc ggctgcgcga cgaccagcag cgtcggccgg
    11461 gcatcgatct cgcggccgac caggaacttc tgcaggttgc cgcccgacag gctgcgcgcc
    11521 ggcgcgtccg gcccgccggc cttcacgccg aagcgcgcg
//
LOCUS       DASVLN010000282         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_176408, whole genome shotgun sequence.
ACCESSION   DASVLN010000282 DASVLN010000000
VERSION     DASVLN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176408"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VF453_06905"
     CDS             <1..587
                     /locus_tag="VF453_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7687415.1"
                     /translation="VATARDDAGLARIAALGAKPLRVDLADAASAAGLAWLVDGEAFD
                     TVIVNAGVYGPATQGLQPPTAADFDAVMHTNVLGAMRALAAVADALAPGAKLGVMSSS
                     MASIAARPAADAWTYRASKAALNSVLKDASIALAGRATCLALDPGWVRTDMGGASAPL
                     APDDAVAGLRRVLAAATPERSGSFLTWRGETMPW"
     gene            685..1815
                     /locus_tag="VF453_06910"
     CDS             685..1815
                     /locus_tag="VF453_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7687416.1"
                     /translation="MHLSDDHRAVQDAIRTYVQERIAPKAAAWDRDAHFPKEELRGLA
                     ELGCYGIAVPTEWDGAGLDYLAVSLILEEIAAGDGATSTVVSVNNCPVCSILMAFGND
                     DQKERFLRPLARGDLLGAFCLTEPHVGSEAGGLRTTAVRDGDDYVLNGVKQFITSGKN
                     GDVAIVMAVTDKAAGKKGISAFVVPTSTPGYVVARVEEKMGQHASDTAQILFENCRVP
                     AANRLGEEGQGLKIALSGLEGGRIGIASQSVGMARAAFEAALRYSKERVAFAQPIFEH
                     QAIQFKLAEMATQIEAARQLIHHAASLKDAGLPCLKEAAMAKLFASEMAERVCSAAIQ
                     VFGGYGYTTDFPVERIYRDVRVTQIYEGTSEVQKILIGRGLA"
     gene            1812..>2008
                     /locus_tag="VF453_06915"
     CDS             1812..>2008
                     /locus_tag="VF453_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687417.1"
                     /translation="MSLGGLVLDDPVAAGLHPRDDWWPDALRLSPGVAFDVAPAPSGI
                     PIAAGALLRPAPSSRRLGAWMR"
BASE COUNT          275 a          748 c          726 g          259 t
ORIGIN      
        1 tggtcgcgac cgcgcgcgac gacgccggcc tcgcgcgcat cgcggcgctg ggcgcgaagc
       61 cgctgcgggt ggatctcgcc gacgccgcga gcgccgcggg cctcgcgtgg ctggtcgacg
      121 gcgaggcctt cgacaccgtg atcgtcaacg caggcgtcta cggccccgcc acgcagggcc
      181 tgcagccgcc gaccgcggcc gacttcgacg ccgtgatgca cacgaacgtg ctgggcgcga
      241 tgcgggcgct cgcggcggtc gcggacgcgc tcgcgcccgg ggcgaagctg ggggtgatgt
      301 cttccagcat ggcctcgatc gccgcgcggc ccgccgccga tgcctggacg taccgcgcct
      361 ccaaggccgc cctcaactcg gtgctgaagg acgcctcgat cgcgctggcc ggccgcgcga
      421 cctgcctcgc gctcgacccc ggctgggtgc gcaccgacat gggcggcgcg tcggccccgc
      481 tggcgccgga cgacgccgtc gccggcctgc gccgggtgct ggccgcggcc acgcccgagc
      541 gatcgggcag cttcctgacc tggcggggcg agaccatgcc ctggtaaggt tccgggtgcg
      601 cgacgcgccc ccgacatgac tgcggccccg cgccacgagc gcccggtccg cgacgctcga
      661 ccacgaccca cgaccgagac caccatgcat ctctccgacg accaccgcgc cgtgcaggac
      721 gccatccgca cctacgtcca ggagcgcatc gcgcccaagg ccgcggcctg ggatcgcgac
      781 gcccacttcc cgaaggagga gctgcgcggg ctcgccgagc tcggctgcta cggcatcgcg
      841 gtgcccaccg agtgggacgg cgcgggcctc gactacctcg cggtgtcgct gatcctcgag
      901 gagatcgccg ccggcgacgg cgcgacgtcc acggtggtga gcgtcaacaa ctgcccggtc
      961 tgctcgatcc tgatggcctt cggcaacgac gaccagaagg agcgcttcct gcggccgctc
     1021 gcgcgcggcg acctgctcgg cgcgttctgc ctgaccgagc cgcacgtcgg ctccgaggcc
     1081 ggcggcctgc gcaccacggc cgtccgcgac ggggacgact acgtcctcaa cggcgtcaag
     1141 cagttcatca ccagcggcaa gaacggcgac gtcgcgatcg tgatggcggt caccgacaag
     1201 gcggccggca agaagggcat cagcgcgttc gtcgtgccga cctcgacgcc gggctacgtc
     1261 gtcgcgcgcg tcgaggagaa gatgggccag cacgcgagcg acacggcgca gatcctgttc
     1321 gagaactgcc gcgtgcccgc cgccaaccgc ctcggcgagg aaggccaggg cctgaagatc
     1381 gcgctgtcgg ggctcgaggg cgggcgcatc ggcatcgcgt cgcagtcggt gggcatggcg
     1441 cgcgcggcgt tcgaggcggc gctgcgctac tcgaaggagc gcgtcgcgtt cgcgcagccg
     1501 atcttcgagc accaggcgat ccagttcaag cttgccgaga tggccacgca gatcgaggcc
     1561 gcgcgccagc tgatccacca cgccgcgagc ctgaaggacg ccggcctgcc ctgcctgaag
     1621 gaagccgcga tggccaagct gttcgccagc gagatggccg agcgcgtgtg ctccgccgcg
     1681 atccaggtgt tcggcggcta cggctacacg accgacttcc cggtcgagcg catctaccgc
     1741 gacgtgcgcg tcacgcagat ctacgagggc acgtccgaag tccagaagat cctcatcggg
     1801 cgggggctgg cgtgagcctg ggcgggctcg tgctcgacga ccccgtcgcc gcgggcctgc
     1861 atccgcgcga cgactggtgg cccgacgcgc tgcgcttgtc gccaggcgtc gcgttcgatg
     1921 tcgcccccgc gccgtcgggc atcccgatcg cggccggcgc gctgctgcgg ccggcgccgt
     1981 cctcgcgccg cctgggtgcg tggatgcg
//
LOCUS       DASVLN010000283         5695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_122184, whole genome shotgun sequence.
ACCESSION   DASVLN010000283 DASVLN010000000
VERSION     DASVLN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122184"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1298)
                     /locus_tag="VF453_06920"
     CDS             complement(39..1298)
                     /locus_tag="VF453_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7687418.1"
                     /translation="MSKPLRALARALALAIIATSAATTACATTTLTVATFPDLDRAAL
                     AAMPRWRAAHPDVDVKIVSLQYADHHTAMTTALATGSGLPDVMAIDFRFIGKFAESNG
                     FEDLSRPPYGDAALAAKFVPYTIAQATNSHGALAALPADIGPGTLLYRTDLLAKAGLA
                     ESDLTGSWEQFIAAGVKLKKATGACLLADSEDITDIVIRAGLKPGEGLYFDRQGHVLV
                     DSPRFVRGFELGRAAHRAGIDAGVTMWTNEWVAGLRSGHIAAEMMGAWLAGHLKNWIA
                     PDTAGKWRSAVLPAGMEASYGGSFYAIPRRAKHKAEAWQFIQFMTADRETQLASLRVL
                     DTFPALIAAQQDPTMAEPMPFLGGQPARLLWRDIAARVPSIAVDKYDAMATDVVRSEY
                     QNVVRDGKDIPTALADAKYLIEHRARR"
     gene            complement(1433..3871)
                     /locus_tag="VF453_06925"
     CDS             complement(1433..3871)
                     /locus_tag="VF453_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7687419.1"
                     /translation="MNSRAPTFRSVLRNPVNLAVLALLAGASSVHAQGAAQPAPAASA
                     AAASRDDAVQEVTVTATKRTTTLQSTPLAVTPINAAELDKEHVQTVEDVVHLVPSFQA
                     TSEGDHGVITMTLRGIGNDSAKTEYADPEVALFVDGIYAPRAEGAAALLFDMDGIEVL
                     RGPQGTLWGRNSTVGAVNMKTAKAEIGSNFGNFEAGLGNYNRLGARGAFNIGISDTFA
                     IRAAFVHEQHDGYVSYQKPPTISLADQETAAAPAIKAYNDAHPNSPQLSFRPLDPNSF
                     VQGGPKYDAQDQTAERISFLWKPMRNLSWNASYEHFEDHGTPNMNLMQDPRPGESFWS
                     ALISVAPYLDRSVNTLRSHVSWDLSDYLNLNYTAGYSRYSGSEDFDQSGGALVPTSFT
                     TNGKVQEDRTDWSHYKNYSHELQLQSNGSHELDWILGLYYAAEDNGIRFDIPITDGTT
                     QGTINWQGAFIQPKETVNSQAVFGQATWNIGDSLHLTGGARFTHDDRKNEGGVNWGWQ
                     GKDGSVPLRIDADTDPATLGAAAFGPTHNDGHYTGSKTTWLARANYDLSKNAMVYASV
                     STGYKSGGLQDGGYHYGPETLTNYEVGSKNTFLDGKVHFNNSLYYEDFKDFQFSAPVT
                     NPDGSHGLATSNAQGAKVYGFETELSAALSKNDTVSISGAYLHTRLGYLLAGSNDYSL
                     PACTESTIISTCLNVTGHKLPHSPSFSGTAMYEHDFRFASGSKLSPRVTFHYEGRSTL
                     SVFDLGPQDEQKAYTRTDVGLRYQDAKDWYVDAFVRNVENGKVRTNAGNSFGAWESQY
                     LPPRTFGVNAGFNF"
     gene            4265..>5695
                     /locus_tag="VF453_06930"
     CDS             4265..>5695
                     /locus_tag="VF453_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX7687420.1"
                     /translation="MNPIRDILVVGGGTAGWLTAAFLARTLGTAAGGVRVTLVEASDI
                     PAIGVGEGTFPSIRSTLSAIGLDEARFLRECDATFKQGVHFAHWRHAPGTAPASEESY
                     FHPFNAPSQRPGGPELLPYWLLGGAGARPFADAVSMQQRVADASRAPKRAGDGDWLGP
                     MNYAYHFDAGKFATLLATHAKSLGVAHVVATVERVELAGPDTIAAVHTRERGALAADL
                     YVDCTGFRAALIGRALGSRPKPLGDVLFVDRALAIQVPYPGPDAPIPSYTISTAHEAG
                     WTWDIGLQRRRGIGYVYSSRHTDDARAEAVLRAYAGPKAEGVAARALKLDIGYRETQW
                     IGNCVAVGLSAGFLEPLESSGIGLIEAAAYLIGFLLPADGDLAPAARRFNAQMRARFE
                     RIVDFLKLHYALTRRTDTAFWRDNADPRSWTPTLSDLLASWRSRPPHRLDFVVDLEMY
                     PPSSWQYVLYGMGYRTDLGALRAAWPR"
BASE COUNT          751 a         1913 c         2110 g          921 t
ORIGIN      
        1 tcggcggcag ggactcggga tgtgtcatcg tggccatctc agcgtcgcgc acggtgttcg
       61 atcaggtatt tcgcgtcggc gagcgcggtc gggatgtcct tgccgtcgcg cacgacgttc
      121 tggtactccg agcgcacgac atcggtggcc atcgcgtcgt acttgtcgac cgcgatcgac
      181 ggcacgcgcg cggcgatgtc gcgccacagc aggcgcgccg gctggccccc gaggaagggc
      241 atgggctcgg ccatcgtcgg gtcctgctgg gccgcgatga gcgcggggaa ggtgtccagc
      301 acgcgcagcg aggcgagctg cgtctcgcgg tcggcggtca tgaactggat gaactgccac
      361 gcctcggcct tgtgcttcgc acgccgcggg atggcgtaga acgagccgcc gtacgaggcc
      421 tccatgcccg caggcagcac ggccgagcgc cacttgccgg cggtgtcggg cgcgatccag
      481 ttcttcaggt ggccggcgag ccaggcgccc atcatctcgg cggcgatgtg gcccgagcgc
      541 aggccggcga cccattcgtt ggtccacatc gtcacgccgg cgtcgatgcc cgcgcggtgc
      601 gcggcccggc cgagctcgaa gccgcgcacg aagcgcggac tgtcgacgag cacgtggccc
      661 tggcggtcga agtacaggcc ctcgcccggc ttgaggccgg cgcggatgac gatgtcggtg
      721 atgtcctcgc tgtcggccag caggcaggcg cccgtggcct tcttcagctt gacgccggcg
      781 gcgatgaact gctcccagct gccggtcagg tcggactcgg ccaggcccgc cttcgcgagc
      841 aggtccgtgc gatagagcag cgtgcccggg ccgatgtcgg ccggcagcgc cgcgagcgcg
      901 ccgtggctgt tggtcgcctg ggcgatggtg taggggacga acttcgcggc aagcgcggcg
      961 tcgccgtagg gcgggcgcga cagatcctcg aagccgttgg actcggcgaa cttgccgatg
     1021 aagcggaagt cgatcgccat cacgtcgggc aggccggagc cggtggcgag cgcggtggtc
     1081 atcgcggtgt ggtggtcggc gtactgcagc gagacgatct tcacgtcgac gtcgggatgc
     1141 gcggcgcgcc agcgcggcat cgcggcgagc gcggcgcggt cgaggtcggg gaaggtcgcg
     1201 acggtgaggg tcgtcgtcgc gcacgcggtc gtggcggccg acgtcgcgat gatcgcgagg
     1261 gccagggcac gggcgagcgc gcgcaggggc ttggacatgg aagcggacgt gagacgggga
     1321 aaaggccgcg ggcgcgggga gacacgggcc cgcggcaaac ccactcggga aagcgcggcc
     1381 tggccgcggg cggcggggcc gcgccgcgcg gcgcaggccc cgcctcgcgg gctcagaagt
     1441 tgaagccggc gttgacgccg aaggtgcgcg gcggcaggta ctgcgattcc cacgcgccga
     1501 agctgttgcc ggcgttggtg cgcaccttgc cgttctcgac gttgcgcacg aaggcgtcca
     1561 cgtaccagtc cttggcgtcc tggtaccgca ggccgacgtc ggtgcgcgtg taggccttct
     1621 gctcgtcctg cggtccgagg tcgaagacgc tcagcgtgct gcggccctcg tagtggaacg
     1681 tcacccgcgg cgagagcttg gagccgctcg cgaagcggaa gtcgtgctcg tacatcgccg
     1741 tgccggagaa cgacggcgag tgcggcagct tgtggcccgt gacgttcagg caggtgctga
     1801 tgatcgtcga ctccgtgcag gcgggcagcg agtagtcgtt gctgccggcg agcaggtacc
     1861 ccaggcgcgt atgcaggtag gcgccgctga tcgacaccgt gtcattcttc gacagcgcgg
     1921 ccgacagctc ggtctcgaag ccgtagacct tggcgccctg cgcgttggag gtcgccaggc
     1981 cgtgcgagcc gtccgggttg gtcaccggcg cgctgaactg gaagtccttg aagtcctcgt
     2041 agtacagcga gttgttgaag tgcaccttgc cgtcgaggaa ggtgttcttg ctgccgacct
     2101 cgtagttggt cagggtctcc gggccgtagt ggtagccgcc gtcctgcagg ccgccggact
     2161 tgtagccggt cgacacgctg gcgtagacca tcgcgttctt gctgaggtcg tagttggcgc
     2221 gcgcgagcca ggtggtcttg gagccggtgt agtggccgtc gttgtgggtc gggccgaagg
     2281 ccgcggcgcc gagcgtggca gggtcggtgt cggcgtcgat gcgcagcggc accgagccgt
     2341 ccttgccttg ccagccccag ttgacgccgc cctcgttctt gcggtcgtcg tgcgtgaagc
     2401 gggcgccgcc ggtcaggtgc aggctgtcgc cgatgttcca ggtcgcctgg ccgaagaccg
     2461 cctgcgagtt caccgtctcc ttgggctgga tgaacgcgcc ctgccagttg atggtgccct
     2521 gggtcgtgcc gtcggtgatg gggatgtcga agcggatgcc gttgtcctcg gcggcgtagt
     2581 agagccccag gatccagtcc agctcgtgcg agccgttgga ctgcagctgc agctcgtggc
     2641 tgtagttctt gtagtgcgac cagtcggtgc ggtcctcctg caccttgccg ttggtcgtga
     2701 agctggtggg caccagcgcg ccgccgctct ggtcgaagtc ctcgctgccg ctgtagcgcg
     2761 agtagcccgc ggtgtagttc aggttgaggt agtcgctcag gtcccagctg acgtggctgc
     2821 gcagggtgtt gacgctgcgg tcgaggtacg gcgcgacgct gatcagcgcc gaccagaagc
     2881 tctcgcccgg acgcggatcc tgcatgaggt tcatgttggg cgtgccgtgg tcctcgaagt
     2941 gctcgtacga ggcgttccag ctcaggttgc gcatgggctt ccacaggaag ctgatgcgct
     3001 cggcggtctg gtcctgcgcg tcgtacttcg ggccgccctg cacgaacgag ttcggatcga
     3061 gcgggcggaa cgagagctgc ggcgagttcg ggtgcgcgtc gttgtaggcc ttgatcgccg
     3121 gggccgcggc ggtctcctgg tcggccagcg agatcgtggg cggcttctgg tagctcacgt
     3181 agccgtcgtg ctgctcgtgc acgaaggccg cgcggatcgc gaaggtgtcg ctgatgccga
     3241 tgttgaacgc gccgcgcgcg cccaggcggt tgtagttgcc caggccggcc tcgaagttgc
     3301 cgaagttgct gccgatctcg gccttggcgg tcttcatgtt gaccgcgccg acggtggagt
     3361 tgcggcccca cagcgtgccc tgcggcccgc gcagcacctc gatgccgtcc atgtcgaaca
     3421 gcagcgccgc ggcgccttcg gcgcgcgggg cgtagatgcc gtcgacgaag agcgcgacct
     3481 cggggtccgc gtactcggtc ttggcgctgt cgttgccgat gccgcgcagg gtcatcgtga
     3541 tgacgccgtg gtcgccttcg ctggtggcct ggaagctcgg cacgaggtgg acgacgtcct
     3601 ccaccgtctg cacgtgctcc ttgtccagct cggcggcgtt gatgggcgtg accgccagcg
     3661 gcgtgctctg cagggtggtg gtgcgcttgg tcgccgtgac ggtgacctcc tgcacggcgt
     3721 cgtcgcgcga ggcggcggcg gcgctcgcgg cgggagcggg ttgcgccgcg ccctgcgcgt
     3781 gcacggagga agcgccggcc agcagggcca gcaccgcgag gttgacgggg ttccggagaa
     3841 cgctccggaa ggtcggtgcg cgagagttca cgtgtgtctc caatgttgct gatgcgtctt
     3901 gcggccgtct atgcggcgat tcatggcgtc ttcgatgtcg cgatgcgcgg gcagcgcggg
     3961 tgcagcaccc cgcggccccc tcctcgccac gtgtcgccac gtgcattccg cggggcgcgc
     4021 gccacggggc cggcgccgct ccgcctcggt cgaccggcct cgtgagcgcg gggtcgaccg
     4081 tcggaatgca gcggatgatg ccgatcgatg acaacgctgt caacaaaaaa gacaacgatg
     4141 tctgacagcg gtgtcatttc gccggtatca tgcccggcaa cgggcggtgg gaccccggga
     4201 aaacgccgag aacgggaagc agcgcccggc ccgcccgtcc cgcaagcgaa agccggagaa
     4261 gcgcatgaat cccattcgag acatcctcgt cgtcggcggc ggcaccgccg gctggctcac
     4321 cgcggccttc ctcgcgcgca cgctgggcac ggccgcgggc ggcgtgcggg tcaccctggt
     4381 cgaggcgagc gacatcccgg ccatcggcgt cggcgaaggc acctttccgt cgatccgcag
     4441 cacgctctcg gcgatcggcc tggacgaggc gcgcttcctg cgcgagtgcg acgcgacgtt
     4501 caagcagggt gtgcacttcg cgcactggcg ccacgcgccg ggcacggcgc cggccagcga
     4561 ggagagctac ttccacccgt tcaacgcccc cagccaacgc ccgggcgggc ccgagctgct
     4621 cccctactgg ctgctgggcg gcgccggcgc acggccgttc gcggacgccg tctcgatgca
     4681 gcagcgcgtg gccgacgcct cgcgcgcgcc caagcgcgcc ggcgacggcg actggctcgg
     4741 gccgatgaac tatgcctacc acttcgacgc cggcaagttc gcgaccctgc tcgcgaccca
     4801 cgcgaagtcg ctcggcgtcg cgcacgtggt ggcgacggtc gaacgcgtcg agctcgcggg
     4861 ccccgacacc atcgccgccg tccacacgcg cgagcgcggc gcgctggcgg ccgacctcta
     4921 cgtcgactgc accggcttcc gcgccgcgct gatcggccgg gccctcggat cgcgcccgaa
     4981 gccgctgggc gacgtgctgt tcgtcgaccg cgcgctcgcg atccaggtcc cgtaccccgg
     5041 ccccgacgcg ccgatcccgt cgtacacgat ctcgaccgcc cacgaggccg gctggacctg
     5101 ggacatcggc ctgcagcggc gccggggcat cggctacgtc tactcgagcc gccacaccga
     5161 cgacgcacgc gccgaggccg tgctgcgcgc ctacgccggg ccgaaggccg agggcgtcgc
     5221 cgcgcgcgcg ctgaagctgg acatcggcta ccgcgagacc cagtggatcg gcaactgcgt
     5281 ggcggtcggg ctgtcggcgg gcttcctcga gccgctcgag tcctcgggca tcggcctgat
     5341 cgaggccgcg gcctacctga tcggcttcct gctgccggcc gacggcgacc tcgcgcccgc
     5401 cgcccgccgc ttcaacgcgc agatgcgcgc gcgcttcgag cgcatcgtcg atttcctgaa
     5461 gctgcactac gccctcaccc ggcgcaccga caccgcgttc tggcgcgaca acgccgaccc
     5521 gcgctcgtgg acgccgacgc tctccgacct gctggcgtcc tggcgctcgc ggccgccgca
     5581 ccggctcgac ttcgtcgtcg acctggagat gtacccgccc tcgagctggc agtacgtgct
     5641 gtacggcatg gggtatcgca ccgacctggg cgcgctgcgc gccgcgtggc cgcgc
//
LOCUS       DASVLN010000284         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_389919, whole genome shotgun sequence.
ACCESSION   DASVLN010000284 DASVLN010000000
VERSION     DASVLN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389919"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1144
                     /locus_tag="VF453_06935"
     CDS             185..1144
                     /locus_tag="VF453_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7687421.1"
                     /translation="MPANPAILSVRRLSKTYASGFQALKSVDLEIRRGEILALLGPNG
                     AGKTTLISIVCGIVNASEGTVTVDGHDIVRDYRATRSLIGLVPQELTTDAFETVWDTV
                     SFSRGLFGKAADPAHIEKTLRALSLWDKKDARIRTLSGGMKRRVMIAKALSHEPQILF
                     LDEPTAGVDVELRRDMWALVRELRATGVTIILTTHYIEEAEEMADRIGVIAKGELLLV
                     EDKAELMDKLGSKQVTLQLAAPLAAVPAAFDGYAVQLSPDGRELTCTYDTRHASHGIV
                     DFMKRVDAAGLGYADLRTKQSSLEEIFVSLVDGARQRKEEKPT"
     gene            1141..1914
                     /locus_tag="VF453_06940"
     CDS             1141..1914
                     /locus_tag="VF453_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7687422.1"
                     /translation="MSALSNWHAIRAIYRFEMARTWRTLTQSIVSPVISTSLYFVVFG
                     AAIGSRIQQVGGIPYGAFIVPGLVMLSLLTQSISNASFGIYFPRFTGTIYELLSAPVS
                     PLEAVIAYVGAAASKSVLLGAIILATAALFVPLRIAHPFVMVLFLVLTAVTFSLVGFI
                     IGLWADGFEKLQVIPLLIVTPLTFLGGSFYSISMLPPFWQTVALFNPVVYLISGFRWS
                     FYGAGDVGVGVSLVVTALFLAAAMAIVAWMFRTGYRLKN"
     gene            2006..2293
                     /locus_tag="VF453_06945"
     CDS             2006..2293
                     /locus_tag="VF453_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687423.1"
                     /translation="MDIRQDPNSIPNFSDGDRLGELQAGEREQEQSSVELDRPDGDGD
                     IPTGSGLDSQLDQGSGVTAKPGASAPGTPAERGEDASKTGGGAVDGLVGAP"
BASE COUNT          337 a          835 c          777 g          352 t
ORIGIN      
        1 tgccgccgga cgaggcgctc gcggtgatgg ccctgatcga actcgggttc gccagcgcga
       61 cccagcgccg cgagctggcg ccggtccagg cctgagcacg ggcgcgcgac gcccgcccat
      121 ccccgcgcag gggcgctggg cgccggcaag cacgagcggc gcgatctcgc tatcctgtcg
      181 cgccatgccc gccaaccccg ccatcctgtc cgtccgccgg ctgtccaaga cctacgcgtc
      241 gggattccag gcgctcaaga gcgtcgacct ggagatccgc cgcggcgaga tcctcgcgct
      301 gctgggtccg aacggcgccg gcaagaccac cctcatcagc atcgtctgcg gcatcgtcaa
      361 cgcgagcgag ggcacggtca ccgtcgacgg ccacgacatc gtgcgcgact accgcgccac
      421 gcgctcgctg atcggcctcg tgccccagga gctcacgacc gacgccttcg agacggtgtg
      481 ggacacggtc tccttcagcc gcggcctgtt cggcaaggcg gccgatccgg ctcacatcga
      541 gaagacgctg cgcgcgctgt cgctgtggga caagaaggat gcgcgcatcc gcacgctctc
      601 gggcggcatg aagcggcgcg tgatgatcgc caaggcgctg tcgcacgagc cgcagatcct
      661 gttcctcgac gagcccaccg cgggcgtcga cgtggagctg cggcgcgaca tgtgggccct
      721 cgtgcgcgag ctgcgcgcca ccggcgtcac catcatcctg acgacgcact acatcgagga
      781 agccgaggag atggccgacc gcatcggcgt catcgccaag ggcgagctgc tgctggtcga
      841 ggacaaggcc gagctgatgg acaagctcgg cagcaagcag gtcacgctgc agctcgccgc
      901 gccgctggcc gcggtgccgg ccgccttcga cggctatgcg gtgcagctgt cgcccgacgg
      961 ccgcgagctc acctgcacct acgacacccg ccacgcgtcc cacggcatcg tcgacttcat
     1021 gaagcgcgtc gacgccgcgg gcctcgggta cgccgacctg cgcaccaagc agagctcgct
     1081 ggaagagatc ttcgtgagcc tcgtcgacgg cgcacggcag cgcaaggagg agaaaccgac
     1141 atgagcgccc tcagcaactg gcacgcgatc cgcgccatct acaggttcga gatggcgcgc
     1201 acctggcgca cgctcacgca gagcatcgtc tcgcccgtga tctcgacctc gctgtacttc
     1261 gtcgtcttcg gcgccgcgat cggctcgcgc atccagcagg tcggcggcat cccgtacggc
     1321 gcgttcatcg tcccggggct ggtgatgctg tcgctgctga cgcagagcat ctccaacgcg
     1381 tcgttcggca tctacttccc gcgcttcacc ggcacgatct acgagctgct gtcggcgccg
     1441 gtgtcgccgc tggaggcggt gatcgcctac gtcggcgcgg cggcgtcgaa gtcggtgctg
     1501 ctgggcgcca tcatcctcgc gacggcggcg cttttcgtgc cgctgcggat cgcgcatccg
     1561 ttcgtcatgg tgctgttcct cgtgctgacc gcggtcacgt tcagcctcgt cggcttcatc
     1621 atcgggctgt gggccgacgg cttcgagaag ctgcaggtga tcccgctgct catcgtcacg
     1681 ccgctgacct tcctgggcgg cagcttctac tcgatctcga tgctgccgcc gttctggcag
     1741 accgtcgccc tgttcaaccc ggtggtctac ctgatcagcg gctttcgctg gagcttctac
     1801 ggcgcgggcg acgtcggcgt gggcgtgagc ctggtggtga cggcgctgtt cctcgctgcg
     1861 gcgatggcca tcgtcgcgtg gatgttccgc accggctatc gcctcaagaa ctgacggccg
     1921 gccgggtccg cggcccgggg aacgcgggtt gccccggcag gacgtgcgcg gcccggcgcc
     1981 gcgcgaacgt tccaggaggc ttcccatgga catccgccaa gaccccaact cgatccccaa
     2041 cttcagcgac ggcgaccgcc tcggcgagct ccaggccggc gagcgcgagc aggagcagtc
     2101 cagcgtcgag ctcgatcgtc cggacggcga tggcgacatc cccaccggct cgggcctgga
     2161 ctcgcagctc gaccagggca gcggcgtgac ggccaagccc ggtgcctcgg cgcccggcac
     2221 ccccgcagag cgcggcgagg acgcctcgaa gaccggcggc ggagccgtcg acggcctggt
     2281 cggcgcgccc tgacgactcg c
//
LOCUS       DASVLN010000285         2790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_68219, whole genome shotgun sequence.
ACCESSION   DASVLN010000285 DASVLN010000000
VERSION     DASVLN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68219"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VF453_06950"
     CDS             complement(<1..408)
                     /locus_tag="VF453_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7687424.1"
                     /translation="MTQADRERHFWPAVWAMGVGGFAIGTGEFVIMGLLPEVARDLGV
                     TIPQAGHVISAYALGVVVGAPVLAVVTASWPRRALLMAMMALFAAGNFASAAAPGYAA
                     MMLLRLVSGLPHGTFFGVSALVAASLAPPGRRAG"
     gene            798..1553
                     /locus_tag="VF453_06955"
     CDS             798..1553
                     /locus_tag="VF453_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX7687425.1"
                     /translation="MPAATAALREGDRVPRVRFRVRDGAEWGEVSSDQLFDGRTVVVF
                     ALPGAFTPTCSSQHLPRYEELAPAFERLGVDEVVCVSVNDPFVMEEWGRSESARRVRL
                     LPDGNAEFTRGMGMLVDKRELNFGARSRRYSMLVRDGRIDKLFLEPDEPGDPYGVSDA
                     DTMLRHLDPRAAAPDEVAILSRPGCPFCAHAKAALDAAGLAYVDISLPPLDRSRALRA
                     MTGAQTVPQVFVNGQLVGDSEALDAWLARRERA"
     gene            1807..>2790
                     /locus_tag="VF453_06960"
     CDS             1807..>2790
                     /locus_tag="VF453_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX7687426.1"
                     /translation="MNSNLFQGRPVAVIGAGVMGIGIVQVALLAGHPVRLFDTRDGAA
                     DAAARQLAQTLAKFADKGKLTPEDVQAALARLRAAATLRELADSALVVEAIVENLAVK
                     QQLLRELEEIVAPECVLASNTSSLSITAIARGLKAPGRVVGMHFFNPVPLMRLVEVVS
                     GLATEPAVADAIHTLAQDWGKVAVHAKSTPGFIVNRIARPFYAETLALLQERAAAPAA
                     LDACVRGAGFRMGPCELMDLIGHDTNFAVTQSVYEANFFDKRFQPSLVQREMVDGGLL
                     GRKSGRGFYHYNDGAAAPKVAEPEAEHPRPAQSLVLHGTGAVAELLAARLGA"
BASE COUNT          376 a         1020 c         1038 g          356 t
ORIGIN      
        1 gcccgcgcgg cggcccggcg gcgcgagcga ggccgccacc agcgccgaga cgccgaagaa
       61 cgtgccgtgc ggcagcccgc tgaccagccg cagcagcatc atcgcggcgt agccgggcgc
      121 cgcggcgctc gcgaagttgc cggccgcgaa gagcgccatc atcgccatca gcagcgcgcg
      181 ccgcggccag ctggccgtca ccacggccag caccggtgcg ccgacgacca cgccgagggc
      241 gtaggcgctg atcacgtggc cggcctgcgg gatcgtgacc cccaggtccc gcgccacctc
      301 cggcagcagg cccatgatca cgaactcgcc ggtgccgatc gcgaagccgc cgacgcccat
      361 cgcccagacc gcgggccaga agtggcgttc gcggtccgcc tgggtcatcg aatggccggc
      421 ggcgggcgcg ggtggaaggc tggcggcgga ggtcgagctc atggcggcag gcggccgcgc
      481 gcgggcgcga caggcgcgtc gcacggcggc aggaaaggcg gttggggagg gagtggcggc
      541 cagaccggcc ggctccgagc gggattgtgc attgcaacaa caaaccttgc gacgcgcgct
      601 ggcatcccgc tggaaccgtt tccggccatt cgtcacgcag tgtgcgatgg cgctgcgcgg
      661 gcgccacggg cccggtccgc atgctgaacc ggacgaggca tccggcttgc cgaccaggga
      721 gatcacgccg accgaccccc accgaaaggc ccgccatgac gagttccgac gccccgacct
      781 ccgcctcccg cgacgacatg ccggccgcga ccgccgccct gcgcgaaggc gaccgcgtcc
      841 cccgcgtgcg cttccgggtg cgcgacggtg cggagtgggg cgaggtgtcc agcgaccagc
      901 tcttcgacgg ccgcacggtc gtcgtcttcg cgttgccggg cgcgttcacg ccgacctgct
      961 cgtcgcagca cctgccgcgc tacgaggagc ttgcgccggc cttcgagcgc ctgggcgtgg
     1021 acgaggtcgt ctgcgtctcc gtcaacgacc cgttcgtgat ggaggagtgg ggccgcagcg
     1081 agtccgcgcg gcgcgtgcgc ctgctgcccg acggcaacgc cgagttcacg cgcgggatgg
     1141 gcatgctcgt cgacaagcgc gagctcaact tcggcgcgcg ctcgcggcgc tattcgatgc
     1201 tggtgcgcga cgggcgcatc gacaagctct tcctcgagcc cgacgaaccg ggcgacccgt
     1261 acggcgtgtc ggacgccgac acgatgctgc gccacctcga cccgcgcgcc gccgcgcccg
     1321 acgaggtcgc gatcctgagc cgccccggct gcccgttctg cgcccacgcg aaggccgcgc
     1381 tcgacgccgc cgggctcgca tacgtggaca tctcgctgcc gccgctggac cgctcgcgcg
     1441 cgctgcgggc gatgaccggc gcccagacgg tgccgcaggt gttcgtcaac gggcagctgg
     1501 tgggtgactc cgaggcgctg gacgcctggc tcgcgcggcg cgagcgggcc tgacgcagcg
     1561 tcgacgaggc gcggcgggac agcgcgcgga tcgagtcggc ccgcgaaaaa tccacccgtc
     1621 gggccgtcgg tcgcgcaaca cagggaggca ggggcgctcg tctcggcccg acaccgcgtg
     1681 cgacgcggcc acgcgggcgg caagggccgc ggcaggatca tcgccgcggt cgggcgcatt
     1741 gtcgggctcg ggggcttgcc tcgtcctgaa gcgggccctt cctgcgtctt gtgcacaatc
     1801 gcccggatga actccaacct cttccagggc cgcccggtgg cggtcatcgg cgcgggcgtg
     1861 atgggcatcg gcatcgtcca ggtggcgctg ctggcgggcc atccggtgcg cctgttcgac
     1921 acgcgcgacg gcgcggccga cgccgcggcg cggcagctgg cgcagacgct ggcgaagttc
     1981 gccgacaagg gcaagctcac gcccgaggac gtgcaggccg ccctcgcgcg cttgcgcgcc
     2041 gcggcgacgc tgcgcgagct cgccgactcg gcgctcgtgg tcgaggcgat cgtcgagaac
     2101 ctcgcggtca agcagcagct gctgcgcgag ctcgaggaga tcgtggcgcc cgagtgcgtg
     2161 ctcgcgagca acacgtcctc gttgtcgatc accgcgatcg cgcgcgggct gaaggcaccg
     2221 gggcgcgtcg tcggcatgca cttcttcaat cccgtgccgc tgatgcggct ggtcgaggtc
     2281 gtctccggcc tcgcgaccga gcccgcggtg gccgacgcga tccacacgct cgcgcaggac
     2341 tggggcaagg tcgcggtgca cgcgaagtcc acgccgggct tcatcgtcaa ccggatcgcg
     2401 cggccgttct acgccgagac gctggcgctg ctgcaggagc gcgcggccgc gcccgcggcg
     2461 ctcgacgcct gcgtgcgcgg cgcgggcttc cgcatggggc cgtgtgagct gatggacctg
     2521 atcggccacg acacgaactt cgcggtcacg cagtccgtct acgaggccaa cttcttcgac
     2581 aagcgcttcc agccttcgct ggtgcagcgc gagatggtcg acggcgggct gctcgggcgc
     2641 aagtcgggcc gcggcttcta ccactacaac gacggcgcgg ccgcgcccaa ggtcgcggag
     2701 ccggaggccg agcacccgcg ccccgcgcag tcgctggtgc tgcacggcac cggggcggtt
     2761 gccgagctgc tcgccgcgcg cctgggggcc
//
LOCUS       DASVLN010000286         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_389930, whole genome shotgun sequence.
ACCESSION   DASVLN010000286 DASVLN010000000
VERSION     DASVLN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389930"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VF453_06965"
     CDS             complement(<1..1060)
                     /locus_tag="VF453_06965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7687427.1"
                     /translation="MDAPPAPADDAGRDARDGVGAGLCAADLEDAEAAGLVGLGVRGD
                     ASPGTDAAPPALVLASLRALSADGPLGREDAAYLERDGQLRMAGAVAQAIEDRATLVV
                     EAGTGVGKTFAYLVPVLLAGRRALVSTATKNLQDQLFYRDLPRLTTALGVPVSTALLK
                     GRSSYLCPYRMERARVEATFADRLAIRALSRIEVWAQSTTSGDLSELEGLDERSEVIP
                     LVTSTRENCLGSECPKFAVCPVMKARRDAMAADVVVVNHHLFFADMALREGGVAELLP
                     SVDVAVFDEAHQMVDIGVQFLGQTLATGQAIDLARDLVGAGLQQARGLAPWTELGAKL
                     ELLARELRLAAAGPRAHAT"
     gene            complement(1077..1280)
                     /locus_tag="VF453_06970"
     CDS             complement(1077..1280)
                     /locus_tag="VF453_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX7687428.1"
                     /translation="MSESLHSPQRRLIELRMEHADLDELIDRAGAQAALDELALKRLK
                     KRRLLLRDHIALLEAELDPPDTA"
     gene            1325..2092
                     /locus_tag="VF453_06975"
     CDS             1325..2092
                     /locus_tag="VF453_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX7687429.1"
                     /translation="MNTTTQGFRLSAATGLHKGDRAYQQDQIELLVHPRIQGCALGLV
                     ADGMGGKSGGRTAADQVVMTARQVFERFSPARDDPTAMLTSLVVESHLMIKLTAITAE
                     EEPHSTLASFLVMPDRRAYTVHAGDSRIYHFRGAELVRRTKDHSYVQRLIDEGRLTEE
                     KANNHPQSNLLTGCLGTQQDPPVELNAIDHLEIGDSLMACSDGLWHYLTAREMGAIIN
                     ALSPREASEMLISKARQRARGSGDNLSLVLVRVEAIA"
BASE COUNT          311 a          786 c          769 g          282 t
ORIGIN      
        1 cggtcgcgtg cgcacgcggg cccgcggcgg ccagtcgcag ctcgcgcgcg agcagctcca
       61 gcttcgcgcc cagctccgtc cacggcgcga ggccgcgcgc ctgctgcagg ccggccccga
      121 cgaggtcgcg cgcgaggtcg atcgcctggc ccgtcgcgag cgtctgcccg aggaactgca
      181 cgccgatgtc gaccatctgg tgcgcctcgt cgaacacggc gacgtcgacc gagggcagca
      241 gctccgcgac gccgccctcg cgcagcgcca tgtcggcgaa gaacaggtgg tggttgacga
      301 cgaccacgtc ggccgccatc gcgtcgcgcc gcgccttcat caccgggcag accgcgaact
      361 tcgggcactc gctgccgagg cagttctcgc gcgtggaggt gaccagcggg atcacctcgg
      421 agcgctcgtc gagcccttcg agctcgctca ggtcgccgct ggtcgtggac tgcgcccaca
      481 cctcgatgcg cgagagcgcg cggatcgcga gccggtcggc gaaggtggcc tcgacgcggg
      541 cccgctccat gcggtacggg cacaggtagc tggagcggcc cttgagcagt gccgtcgaga
      601 cggggacgcc cagggcggtc gtcaggcgcg gcaggtcgcg gtagaagagc tggtcctgca
      661 ggttcttggt cgccgtgctg acgagcgcgc gccgcccggc cagcagcacg ggcacgaggt
      721 aggcgaaggt cttgccgacg ccggtgcccg cctcgaccac cagcgtcgcc cgatcctcga
      781 tcgcctgcgc gaccgccccg gccatgcgca gctggccgtc gcgctcgagg taggcggcgt
      841 cctcgcgccc gagcgggccg tcggccgaca gcgcgcgcag cgaagcgagc acgagcgcgg
      901 gcggcgccgc gtccgtcccg ggggatgcat cgccacggac accaagcccg accaggccgg
      961 cggcctcggc atcctcgaga tccgccgcgc acaggccggc accgaccccg tcgcgcgcat
     1021 cgcggccggc gtcgtctgca ggggcgggcg gcgcgtccat cgcgtccggg cgcgcctcag
     1081 gcggtgtcgg ggggatcgag ttcagcttcg agcagcgcga tgtggtcgcg caggaggagg
     1141 cgccgcttct tcaggcgctt gagggcgagt tcgtcgagcg ccgcctgcgc gcccgcacgg
     1201 tcgatcagct cgtcgaggtc ggcatgctcc atgcggagct cgatcagccg tcgttgcgga
     1261 gaatgcaggg attcgctcat gtcggggccc gcgccgggca tcgccagagt ttatattgac
     1321 gaccatgaac acgaccaccc aaggcttccg actttccgcc gccacgggac tgcacaaggg
     1381 cgaccgcgcc taccagcagg accagatcga gctcctggtg cacccgcgca tccagggctg
     1441 cgcgctgggg ctggtggcgg acggcatggg cggcaagagc ggtggccgca ccgccgcgga
     1501 ccaggtcgtg atgacggcgc gccaggtgtt cgagcgcttc tcgcccgcgc gcgacgatcc
     1561 gaccgcgatg ctcaccagcc tggtcgtcga gtcgcacctg atgatcaagc tgaccgcgat
     1621 caccgccgag gaggagccgc acagcacgct cgcgtccttc ctcgtgatgc cggatcgccg
     1681 cgcctacacc gtgcacgcgg gcgattcgcg catctaccac ttccgcggcg ccgagctcgt
     1741 gcgccgcacc aaggaccact cctacgtcca gcgcctcatc gacgagggcc ggctgacgga
     1801 ggagaaggcc aacaaccacc cgcagtccaa tctgctgacc ggctgcctcg gcacccagca
     1861 ggatccgccg gtcgagctca acgcgatcga ccacctcgag atcggcgact cgctgatggc
     1921 ctgcagcgat ggcctgtggc actacctgac cgcgcgcgag atgggtgcga tcatcaacgc
     1981 gctgtcgccg cgcgaggcga gcgagatgct gatcagcaag gcgcgccagc gggcgcgcgg
     2041 atccggcgac aacctctcgc tggtgctggt gcgggtcgag gcgatcgcct gaggcttcgc
     2101 ccccgcgtgc gccaccccgg cgcggcggcg tgcgccgcga ccgccgcc
//
LOCUS       DASVLN010000287         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_499737, whole genome shotgun sequence.
ACCESSION   DASVLN010000287 DASVLN010000000
VERSION     DASVLN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499737"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VF453_06980"
     CDS             <1..1029
                     /locus_tag="VF453_06980"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX7687430.1"
                     /translation="EAAAAAARAVGVGSGASPMVSGHSEANAALEEELAAFVGLPRAL
                     YFYAGYATNAGVVPALVGPGDALFSDALNHACLIDGARLSKATLHRFAHADLAELETQ
                     LAASPARRKLVISDAVFSMDGDVADVPALLALCERHDALLMLDDAHGFGVLGPQGRGT
                     LAAAGFANLDGQRGASRRVLYMATLGKAAGVSGAFVAGPDTLVEWVLQKTRSYIFATA
                     APALLAESLRAALRVMRAEPERLARLHANIARFKAGVPAAIAGTGWTLLPSSTAVQGL
                     VIGSNALALRVMQSLADDGLWVPAIRPPTVPEGTARLRIALSAAHTPAEVDRLLAALA
                     NAARRHAA"
     gene            complement(1163..>1611)
                     /locus_tag="VF453_06985"
     CDS             complement(1163..>1611)
                     /locus_tag="VF453_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687431.1"
                     /translation="AQARQREQQEAQQRSQQAAQQHAQQEAAQAQARQREQQEAQQRS
                     QQAAQQHAQQEAAQAQARQREQQEAQQRSQQAAQQHAQQEAAQAQARQREQQEAQQRS
                     QQAAQQHAQQEAAQAQARQREQAQKEQAERKEQQQRRDDNNGQNQR"
BASE COUNT          140 a          627 c          592 g          252 t
ORIGIN      
        1 gaggccgcgg ccgccgcggc gcgcgcggtc ggcgtcggca gcggcgcctc gccgatggtc
       61 agcggccaca gcgaggccaa cgcggcgctc gaggaggagc tcgccgcctt cgtcgggctg
      121 ccgcgggcgc tgtacttcta cgccggctac gcgacgaacg cgggcgtcgt gccggcgctg
      181 gtcgggcccg gcgacgcgct gttctccgac gcgctcaacc acgcctgcct catcgacggc
      241 gcacgcctgt ccaaggcgac gctgcaccgc ttcgcgcacg ccgacctcgc cgagctcgag
      301 acccagctcg ccgccagccc ggcgcgtcgc aagctcgtga tcagcgacgc ggtcttcagc
      361 atggacggcg acgtcgccga cgtccccgcg ctgctggccc tgtgcgaacg ccacgacgcg
      421 ctgctgatgc tggacgacgc gcacggcttc ggcgtgctcg ggccgcaagg gcgcgggacg
      481 ctcgcggccg cggggttcgc gaatctcgac gggcagcgcg gcgcgagccg gcgcgtgctg
      541 tacatggcca cgctcggaaa ggcggcgggc gtctccgggg cgttcgtcgc cgggcccgac
      601 acgctggtcg aatgggtgct gcagaagacg cgcagctaca tcttcgccac cgctgcgccg
      661 gcgctgctgg ccgagtcgct gcgcgccgcg ctgcgcgtga tgcgcgcgga gccggagcgg
      721 ctcgcccgcc tgcacgccaa catcgcgcgt ttcaaggccg gcgtgccggc ggcgatcgcg
      781 ggcaccggct ggacgctgct gccctcgtcg accgcggtgc agggcctggt catcggcagc
      841 aacgcgctcg cgctccgcgt catgcagtcg ctcgccgacg acggcctctg ggtgcccgcc
      901 atccgcccgc ccaccgtccc cgagggcacg gcgcgcctgc gcatcgccct gtcggccgcg
      961 cacacgccgg ccgaggtcga ccggttgctc gcggcgctcg cgaacgcggc gcggcgacac
     1021 gcggcctgag gcgagatccc tcctcgtgat ccggagcccg gcgacatcgt ggtgccggcg
     1081 accggcgtcg tttccatcga caccggtcga cgagccggcc gcctgagcgc gcgcccgacg
     1141 ggccgcggcc ggcgccgccg cgtcagcgct ggttctggcc gttgttgtcg tcgcggcgct
     1201 gctgctgctc cttgcgctcg gcctgctcct tctgcgcctg ctcacgctgg cgcgcctggg
     1261 cctgggcggc ttcctgctgc gcgtgctgct gcgcggcctg ctgcgagcgc tgctgcgctt
     1321 cctgctgctc gcgctggcgc gcctgcgcct gcgcggcctc ctgctgcgcg tgctgttgcg
     1381 cggcctgctg cgaacgttgc tgcgcttcct gctgttcgcg ctggcgcgcc tgcgcctgcg
     1441 cggcttcctg ctgcgcgtgc tgttgcgcgg cctgctgcga gcgctgctgc gcttcctgct
     1501 gttcgcgctg gcgcgcctgc gcctgcgcgg cctcctgctg cgcatgctgt tgcgcggcct
     1561 gctgcgagcg ctgctgcgct tcctgctgct cgcgctggcg cgcctgcgcc t
//
LOCUS       DASVLN010000288         8294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_499806, whole genome shotgun sequence.
ACCESSION   DASVLN010000288 DASVLN010000000
VERSION     DASVLN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499806"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..734)
                     /locus_tag="VF453_06990"
     CDS             complement(15..734)
                     /locus_tag="VF453_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7687432.1"
                     /translation="MSDSILSVSGLSVNYGHIEAVRDVDFALAAGRITTLVGANGAGK
                     STTLLALSGLIPKAAGKVMFDGHDVTRLAAHKLVGAGLVQVAEGRATLTTLSVRENLE
                     LGAYTRRDGAAARAGDLERVFALFPRLKEREDGLAGNLSGGEQQMLAIGRALMARPRV
                     LLLDEPSMGLAPIVVQGIFRTLQEINRDGLTIFLVEQNVRQALKIAERAYVLENGRIV
                     LQGGGRELLDEPRVREAYLGG"
     gene            complement(731..1546)
                     /locus_tag="VF453_06995"
     CDS             complement(731..1546)
                     /locus_tag="VF453_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7687433.1"
                     /translation="MSERAASDLLRLDGVSRHFGGLKVLQDVSFTVRPGTISGLIGPN
                     GAGKTTVFNLITGLLPPSDGAITFDGESLVGRKPHQITRRGVARTFQNIRIFKEMTLL
                     ENVLVGTHRHLGYGVPGLLMSLPGFRAEERRARERARELLSWMKLDHKADDLADNLSY
                     GAQRKLEIARALATEPRLLLLDEPVAGMNSTEKTELMREIVNIQGRGYTIFMIEHDMR
                     FVMGLCEHISVLNFGRIIARGGPEQIRTDPQVIEAYLGREDDDAPAAQAEAAR"
     gene            complement(1543..2403)
                     /locus_tag="VF453_07000"
     CDS             complement(1543..2403)
                     /locus_tag="VF453_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7687434.1"
                     /translation="MDAFDNFWSIYSNVVLSLGTNALLALSIYLTLSCGMLAMANAAF
                     MGIGAYTSAILTMNYGAPFPLAIAAGMAAPAVVAFVIGKPTLRLSGVYLAMATLAFGE
                     VVRIVILNMDSLTGGALGLNGIPQSTQWWHVLLAVAIVLFVLGRLRRSKIGRAFEAIK
                     EDETAAGLMGIDVNGHKMLAFVLGAAIAGLAGVLDAHLTFFIGPQEFGFDRGVEILTM
                     TILGGINSLVGPVVGSVIVTLLPELLRGLQDYRGVFNGLILVAIVLFLPKGLWNPAWL
                     KRRVRAKEAA"
     gene            complement(2509..3390)
                     /locus_tag="VF453_07005"
     CDS             complement(2509..3390)
                     /locus_tag="VF453_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7687435.1"
                     /translation="MLEQQLFNALSLGCVYALFALGFTLVFGVLGVINLSHGAVFMAG
                     AYAALAAVTRLHAPLWAAILAGFAFSGLLGLAIDRLVLRPLRARDAPHLIPMIATIGL
                     GITMNNLAQGLFGAENQRFPSDVLPQDSLTIAGVSVTVLELGIILVSFALMTVLLVTL
                     KKTQFGRALRAIAESPKAARLLGIDVEGLFMVTSFVAAGLGGIAGVLIGLYSNALFPL
                     MGQPIMHKGIAVIILGGMGDIRGAMFAGLFLGFAEVLSVAYVGSDMRDAVAFGLLFLV
                     LLVRPKGLFGRIQERKV"
     gene            complement(3407..4483)
                     /locus_tag="VF453_07010"
     CDS             complement(3407..4483)
                     /locus_tag="VF453_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7687436.1"
                     /translation="MKIGVAEALSGGAAQYGTKIRNGFQLAVDEINAAGGVNGNKIVL
                     VVEDEQGKKDEAINAFKKLIFQDKVLMLFGPTLSNSAQAADPIAQGSHIVVFGTSNTA
                     DGITSIGDHVFRNSVTEADVLPVTIKTAAKVAGIKKVAVLYGNDDVFTKSGYDNFKKA
                     LEELHIPVTTTETFAKGDVDFKAQLTKIKATNPDALVLSALIAEGAPIMVQARQLGLN
                     VPVIGGNGMNSVKIFDLAKGASDGLWVGSPWSIENRTPQNNSFIVAYTKQFSDPPDQF
                     AAQAYDAMHIAVEALKKVKLSGNLAKDRDAVQAALPAIKWTGATGPFSFRRATDKSGK
                     PAGYDANQVPIVSTTRGKNYEIEK"
     gene            4880..7069
                     /locus_tag="VF453_07015"
     CDS             4880..7069
                     /locus_tag="VF453_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEX7687437.1"
                     /translation="MLALAAAPAWSQTATTPGADSAGTAGAAAAGLVANQSVTIEGEA
                     QEDFGRGSVSLDKLPADLHEVPQSITVLSKSLLQSQGANSLADALRNVPGITLGGAEG
                     GQIGNNINLNGFTARTDLYLDGFRDRGQYYRDTFALDSVEVLMGPSSMLFGRGSTGGA
                     INQVSKVAQLRPVTELELSATTNGLARIAGDIDRRVGDTAAFRVAAMAQEGAPTTRDV
                     MRNRDAGVAPSLRFGIGTGDEITLSALLQANRDMPDYGVSPRTFAGRVGRPVDVPRDT
                     FFGYKDDRTIQDVGALTAQWQHRFGADTILRERLQTNRVHVDARETASQGLLGSGAGG
                     AVEDTDPLSALYVRLQSHDRVIDDHSVFDQADLTSRFDTGPVHHELLAGAELGVETYS
                     NQAYSRTGSGSCDGVKLGTGYVGCELLTQPVHVDSPASAPDVAGNLATSHADSTALYV
                     GDTLAAGPFKLVLGVRRDWFDARVANTIPTASAPASVAQDVAHTSYRGGAIWQPTPLQ
                     SYYVSYSTSFDPSLEQLTNTTGATRPLPPASNKAIEGGGRVDVVEGRLSVNGAAFQIT
                     QDNARNQQADGTYAATGSVRVRGVRLGASGAPVRGWKVFAGYAWLDAEIVRAIAVGTQ
                     GMVPANTPRNSATLWTTWEFLPRFEIGGGAVYQSRRYMNNTNLTEVPGYARIDATLAY
                     RRRDYDVRLNLFNLADRKWYDGIIQSDGGRAVPGTGRSAMLSLSYHL"
     gene            7074..7760
                     /locus_tag="VF453_07020"
     CDS             7074..7760
                     /locus_tag="VF453_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX7687438.1"
                     /translation="MLVTIPQALDADRLAAVRARLETADDAWVDGRVTAGWQGAPVKS
                     NEQVDERSPIAAECQALVLAALERHPRFISAVLPNEVYPPMFNRYGAGMAFGAHVDGS
                     VRLHPRTGRKLRTDVSATLFLNEPSDYDGGELVIHDAYGTQRVKLAAGDMVVYPATSL
                     HEVTPVTRGTRLASFFWVQSLVRDDARRAMLFDLDNAIQRLNRQGADDEARRVLVGVY
                     HNLMRQWSET"
     gene            7834..>8294
                     /locus_tag="VF453_07025"
     CDS             7834..>8294
                     /locus_tag="VF453_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX7687439.1"
                     /translation="MTTPDPRRGAPAPRISRRMAAVAWLRKTHGWIGLWGATLGLLFG
                     FSGFWLNHRAVLKLEPEVTRNQAQIAVPDPGPADAKTMAAWLQLVLPIARQATSIRVE
                     PAHPVAWADSPGARSAPAAVDGQSALAGVGLERSRSRERETDPGIAPASAAS"
BASE COUNT         1246 a         2936 c         2920 g         1192 t
ORIGIN      
        1 gaacgccgcg gcgttcagcc gcccaggtag gcttcccgca cgcgcggctc gtccagcagc
       61 tcgcgcccgc cgccctggag cacgatgcgg ccgttctcca ggacgtaggc gcgctcggcg
      121 atcttgagcg cctggcgcac gttctgctcg acgaggaaga tcgtgagccc gtcgcggttg
      181 atctcctgca gcgtgcggaa gatgccctgc acgacgatcg gcgcgaggcc catcgacggc
      241 tcgtccagca gcagcacgcg cgggcgcgcc atcagcgcgc ggccgatcgc gagcatctgc
      301 tgctcgccgc ccgagaggtt gcccgccagg ccgtcctcgc gctccttcag gcgcgggaag
      361 agggcgaaga cccgctcgag gtcgcccgcg cgcgcggcag cgccgtcgcg gcgggtgtag
      421 gcgccgagct ccaggttctc gcgcacgctc aacgtcgtca gcgtggcgcg gccctcggcc
      481 acctgcacca ggccggcgcc gaccagcttg tgcgcggcca ggcgcgtgac gtcgtggccg
      541 tcgaacatca ccttgccggc ggctttcggg atcagccccg acagcgcgag cagcgtcgtc
      601 gactttcccg cgccgttggc gccgaccagc gtcgtgatgc ggcccgcggc gagcgcgaag
      661 tcgacgtccc ggacggcctc gatgtggccg tagttgacgc tcaggccgct gaccgacagg
      721 atgctgtcgc tcatcgcgcc gcctccgcct gcgcggccgg cgcgtcgtcg tcctcgcggc
      781 cgaggtaggc ctcgatcacc tgcgggtcgg tgcggatctg ctccgggccg ccgcgggcga
      841 tgatgcggcc gaagttcagc accgagatgt gctcgcacag gcccatcacg aagcgcatgt
      901 cgtgctcgat catgaagatg gtgtagccgc ggccctggat gttgacgatc tcgcgcatca
      961 gctccgtctt ctcggtgctg ttcatgccgg cgacgggctc gtcgagcagc agcaggcgcg
     1021 gctcggtggc cagcgcgcgc gcgatctcga gcttgcgctg cgcgccgtag gacaggttgt
     1081 cggcgaggtc gtcggccttg tggtcgagct tcatccacga cagcagctcg cgcgcgcgct
     1141 cgcgggcgcg gcgctcctcc gcgcggaagc ccggcagcga catcagcagc cccggcacgc
     1201 cgtagcccag gtggcggtgc gtgccgacga gcacgttctc cagcagcgtc atctccttga
     1261 agatgcggat gttctggaac gtgcgcgcga cgccgcggcg cgtgatctgg tgcggcttgc
     1321 ggccgacgag gctctcgccg tcgaaggtga tcgcgccgtc gctgggcgga agcaggcccg
     1381 tgatgaggtt gaagaccgtg gtcttgcccg cgccgttggg cccgatgagg ccggagatgg
     1441 tgccgggccg gaccgtgaac gagacgtcct gcagcacctt cagcccgccg aagtggcgcg
     1501 agacgccgtc caggcgcaag aggtcggagg ccgcgcgctc gctcatgcgg cctccttcgc
     1561 gcgcacgcgg cgcttgagcc acgcgggatt ccacagcccc ttgggcagga acagcacgat
     1621 cgccaccagg atcaggccgt tgaagacgcc gcggtagtcc tgcaggccgc gcagcagctc
     1681 gggcagcagc gtgacgatga cggagccgac cacggggccg acgaggctgt tgatgccgcc
     1741 gaggatggtc atggtgagga tctcgacgcc gcggtcgaag ccgaactcct gcgggccgat
     1801 gaagaaggtg aggtgggcgt ccagcacgcc ggccaggccc gcgatggcgg cgccgagcac
     1861 gaaggccagc atcttgtggc cgttgacgtc gatgcccatc aggcccgcgg cggtctcgtc
     1921 ctccttgatg gcctcgaagg cgcggccgat cttcgagcgc cgcagccgcc cgagcacgaa
     1981 cagcacgatc gccaccgcga gcagcacgtg ccaccactga gtggactgcg ggatgccgtt
     2041 cagtcccagc gcgccgccgg tgaggctgtc catgttgagg atgacgatgc gcaccacctc
     2101 gccgaacgcc agcgtggcca tcgcgaggta gacccccgac agccgcagcg tcggcttgcc
     2161 gatcacgaac gcgaccaccg ccggcgccgc cattcccgcc gcgatcgcga gcgggaaggg
     2221 cgcgccgtag ttcatggtca ggatcgccga ggtgtacgcg ccgatgccca tgaaggcggc
     2281 gttggccatc gccagcatcc cgcacgacag ggtgaggtag atggacagcg ccagcagcgc
     2341 attggtgccg agcgacagca ccacgttgct gtagatcgac cagaagttgt cgaaggcgtc
     2401 gatcattggt tgcctgcgcg gcatccgccg gcgagcgctc ggccgctccg gagcgagccg
     2461 gcgggccccc tcggggggcg atgagcggag cgaatggggg tggtcatgtc agactttgcg
     2521 ttcctggatg cggccgaaca ggcccttggg acgcaccagc agcacgagga acagcaggcc
     2581 gaaggcgacg gcgtcgcgca tgtcggagcc gacgtacgcg accgacagca cctccgcgaa
     2641 cccgaggaac aggcccgcga acatcgcgcc gcggatgtcg cccatgccgc ccaggatgat
     2701 caccgcgatg cccttgtgca tgatgggctg gcccatcagc gggaagaggg cgttcgagta
     2761 caggccgatc agcacgcctg cgatgccgcc cagccccgcc gcgacgaagg aggtgaccat
     2821 gaacaggccc tcgacgtcga tgcccagcag ccgcgcggcc ttggggctct cggcgatcgc
     2881 gcgcagcgcg cggccgaact gcgtcttctt cagcgtgacg agcagcaccg tcatcagtgc
     2941 gaaggacacg aggatgatgc ccagctccag caccgtcacg ctgacgccgg cgatggtcag
     3001 cgagtcctgc ggcagcacgt cgctcgggaa gcgctggttc tcggcgccga acagcccctg
     3061 cgcgaggttg ttcatcgtga tgcccagccc gatggtggcg atcatgggga tcaggtgcgg
     3121 cgcgtcgcgc gcgcgcagcg gccgcagcac gaggcggtcg atggcgagcc cgagcagccc
     3181 cgagaacgcg aacccggcga ggatggccgc ccacagcggc gcatgcaggc gcgtgaccgc
     3241 ggccagcgcc gcgtaggcgc cggccatgaa gacggcgccg tgcgagaggt tgatcacccc
     3301 caggacgccg aagacgagcg tgaagcccag cgcgaacagc gcgtagacgc aaccgagcga
     3361 gagggcgttg aagagctgct gttcgagcac gggcgcgcgc gtcctgttac ttctcgatct
     3421 cgtagttctt gccgcgcgtg gtgctcacga tgggcacctg gttcgcgtcg tagccggccg
     3481 gcttgccgga cttgtccgtc gcgcggcgga acgagaaggg gccggtcgcg ccggtccact
     3541 tgatggcggg cagcgcggcc tgcacggcgt cgcggtcctt ggcgaggttg ccgctcagct
     3601 tgaccttctt caacgcctcg acggcgatgt gcatcgcgtc atacgcctgc gccgcgaact
     3661 ggtcgggcgg gtcgctgaac tgcttggtgt aggcgacgat gaagctgttg ttctgcggcg
     3721 tgcggttctc gatcgaccac gggctgccga cccacaggcc gtccgaggcg cccttggcga
     3781 gatcgaagat cttgaccgag ttcatgccgt tgccgccgat cacgggcacg ttcaggccca
     3841 gctggcgcgc ctgcaccatg atgggcgcgc cttcggcgat cagcgcggac agcacgagcg
     3901 cgtcggggtt ggtcgccttg atcttggtca gctgcgcctt gaagtcgaca tcgcccttcg
     3961 cgaaggtctc ggtggtggtg accgggatgt gcagctcttc cagcgccttc ttgaagttgt
     4021 cgtagccgct cttggtgaag acgtcgtcgt tgccgtacag cacggcgacc ttcttgatgc
     4081 ccgcgacctt ggccgcggtc ttgatcgtca ccggcagcac gtcggcctcg gtgaccgagt
     4141 tgcggaagac gtggtcgccg atggaggtga tgccgtcggc cgtgttggac gtgccgaaga
     4201 ccacgatgtg gctgccctgg gcgatcgggt cggccgcctg cgccgagttc gacagggtcg
     4261 ggccgaacag catcagcacc ttgtcctgga agatcagctt cttgaaggcg ttgatcgcct
     4321 cgtccttctt gccctgctcg tcctcgacga ccagcacgat cttgttgccg ttgacgccgc
     4381 cggccgcgtt gatctcgtcg accgccagct ggaagccgtt gcggatcttc gtgccgtact
     4441 gcgcggcgcc gccggagagc gcttccgcga cgccgatctt gatgtcggcc gcctgggcgc
     4501 cggcagcgcc gaggaacgcg gccgcggcga gcgcgacggg gagggcccgg gcgaaacgga
     4561 ggggggatgt cacttgggat gtctccatgg cggattcgat gaaccctgaa atgtaagcgg
     4621 tcatggccga ggtggcacgg ccggcggaca ttgtggcgtc gctggcgtcg cgccgggttc
     4681 acatgttggc gcccgggagc gcgcggcggc cgtcgtggcg ctcggcccgg tcgccgttgc
     4741 gaggagatga cagtgcgggc gagatggcga ctcggcaggg gttgcctgtc acatcgaaat
     4801 gaacaagaat gattcgcatt aacatttcag ccatccgaac tcgtcgatga accgccgccc
     4861 gacccctatc gccgccgcct tgctcgccct tgccgccgcg cccgcctggt cgcagaccgc
     4921 caccaccccc ggcgccgact cggccggcac cgccggcgcc gcggcggccg ggctggtcgc
     4981 caaccagagc gtgacgatcg agggcgaggc gcaggaggat ttcggccgcg gcagcgtgtc
     5041 gctcgacaag ctgccggcgg acctgcacga ggtgccgcag tcgatcaccg tgctgagcaa
     5101 gtcgctgctg cagagccagg gcgcgaactc gctggccgac gcgctgcgca acgtgcccgg
     5161 catcacgctc ggcggcgcgg agggcgggca gatcggcaac aacatcaacc tcaacggctt
     5221 caccgcgcgc acagacctct acctggacgg cttccgcgac cgcggccagt actaccgcga
     5281 caccttcgcg ctcgactcgg tcgaggtgct gatggggccg tcgtcgatgc tgttcggccg
     5341 cggctccacg ggcggcgcga tcaaccaggt gagcaaggtg gcgcagctgc gcccggtgac
     5401 cgagctcgag ctctcggcca ccaccaacgg gctcgcgcgc atcgcgggcg acatcgatcg
     5461 ccgggtcgga gacaccgcgg ccttccgcgt cgccgcgatg gcccaggagg gcgcgcccac
     5521 gacgcgcgac gtgatgcgca accgcgatgc cggcgtcgcg ccctcgctgc gcttcggcat
     5581 cggcacgggc gacgagatca cgctgtccgc gctgctgcag gccaaccgcg acatgcccga
     5641 ctacggcgtc tcgccgcgca ccttcgcggg ccgggtcggg cggcccgtgg acgtgccgcg
     5701 cgacaccttc ttcggctaca aggacgaccg cacgatccag gacgtcggcg cgctcacggc
     5761 gcagtggcag caccggttcg gcgccgacac gatcctgcgc gaacgcctgc agaccaaccg
     5821 cgtgcacgtc gacgcgcgcg agaccgcgtc gcagggcctg ctgggcagcg gcgccggcgg
     5881 cgcggtcgag gacaccgatc cgctgtccgc gctctacgtg cgcctgcaga gccatgaccg
     5941 cgtgatagac gaccactcgg tcttcgacca ggccgacctg acgagccgat tcgacaccgg
     6001 gccagtccac cacgagttgc tggcgggggc ggagctcggc gtcgagacct actcgaacca
     6061 ggcctacagc cgcaccggca gcggctcgtg cgacggcgtc aagctgggca ccggctacgt
     6121 gggatgcgag ctgctgacgc agcccgtcca cgtcgactcc ccggcctcgg cgcccgacgt
     6181 cgcgggcaac ctcgcgacca gccacgccga ctccacggcg ctgtacgtcg gcgacacgct
     6241 ggccgccggc ccgttcaagc tcgtgctcgg cgtgcgccgg gactggttcg acgcccgcgt
     6301 cgccaacacg atcccgacgg cgagcgcgcc cgcgagcgtc gcgcaggacg tcgcgcacac
     6361 gagctaccgc ggcggcgcga tctggcagcc gacgccgctg cagtcctact acgtcagcta
     6421 cagcacctcg ttcgacccgt cgctggagca actgaccaac accaccggcg cgacgcggcc
     6481 gctgccgccc gcgagcaaca aggcgatcga gggcgggggc cgggtcgacg tcgtcgaggg
     6541 ccggctgtcg gtcaacggcg cggcgttcca gatcacgcag gacaacgcgc gcaaccagca
     6601 ggccgacggc acctacgccg cgaccggcag cgtgcgcgtg cgcggcgtgc gcctgggtgc
     6661 ctcgggggcg ccggtcaggg gctggaaggt cttcgcgggc tacgcgtggc tcgatgccga
     6721 gatcgtgcgc gcgatcgcgg tcggcaccca gggcatggtg ccggccaaca cgccgcgcaa
     6781 ctccgccacg ctgtggacga cgtgggagtt cctcccccgt ttcgagatcg gcggcggagc
     6841 cgtctaccag tcgcggcgct acatgaacaa caccaacctg accgaggtgc cgggctatgc
     6901 gcgcatcgac gcgacgctcg cgtaccgccg gcgcgactac gatgtgcgcc tgaacctgtt
     6961 caacctcgcg gaccgcaagt ggtacgacgg catcatccag tccgacggcg gccgcgccgt
     7021 gccgggcacc ggccgcagcg cgatgctctc gttgtcctac catctctgac agcatgctcg
     7081 tcacgatccc gcaggcgctg gacgccgacc gactcgccgc cgtgcgtgcc cggctggaga
     7141 ccgcggacga cgcctgggtc gacggccgcg tcaccgccgg ctggcagggc gcgccggtca
     7201 agtccaacga gcaggtggac gagcgctcgc ccatcgcggc ggagtgccag gcgctggtcc
     7261 tcgcggcgct ggaacgccat ccccgcttca tcagcgccgt gctgcccaac gaggtctacc
     7321 cgccgatgtt caaccgctac ggcgccggca tggccttcgg cgcccacgtg gacggcagcg
     7381 tgcggctgca tccgcgcacg ggccgcaagc tgcgcaccga cgtctccgcg acgctgttcc
     7441 tcaacgagcc ttcggactac gacggcggcg agctcgtgat ccacgacgcc tacggcacgc
     7501 agcgcgtgaa gctggcggcc ggcgacatgg tggtgtaccc ggcgaccagc ctgcacgagg
     7561 tcacgcccgt cacgcgcggc acgcgtctcg cgtcgttctt ctgggtgcag agcctcgtgc
     7621 gcgacgacgc ccggcgggcg atgctgttcg acctcgacaa cgcgatccag cgcctgaaca
     7681 ggcagggcgc cgacgacgag gcccgccgcg tgctggtcgg cgtctaccac aacctgatgc
     7741 ggcaatggag cgagacctga gcgtcgcgcg gcaccgctat cctcgaccga cctgccgccg
     7801 cccggcggtc cctgcagacg agttcccgct cccatgacga ctcccgaccc gcgccgcggc
     7861 gcgcccgctc cccgcatcag ccgccgcatg gccgccgtcg cctggctgcg aaagacccac
     7921 ggctggatcg gcctgtgggg cgcgaccctg ggccttctgt tcggcttcag cggcttctgg
     7981 ctcaaccatc gcgccgtgct caagctcgag cccgaggtca cgcgcaacca ggcgcagatc
     8041 gcggtgcccg atcccggccc cgcggacgcg aagacgatgg ccgcctggct gcagctcgtg
     8101 ctgcccatcg cccgccaggc caccagcatc cgcgtcgagc ccgcgcatcc ggtcgcctgg
     8161 gccgacagcc cgggcgcccg atccgcaccg gccgcggtcg acggccagtc cgcgctcgcg
     8221 ggcgtcggcc tggagcgcag ccgttcgcgc gaacgcgaga ccgatcccgg catcgcgccg
     8281 gcgtccgcgg cctc
//
LOCUS       DASVLN010000289         8967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_445471, whole genome shotgun sequence.
ACCESSION   DASVLN010000289 DASVLN010000000
VERSION     DASVLN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445471"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF453_07030"
     CDS             <1..230
                     /locus_tag="VF453_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687440.1"
                     /translation="TADARAPQASLPQGLWDSLGLAAGDKVRVTQGEGSVVLAARLDK
                     TLAPTAVRVPAGHVLTAALGAAFGPITVAKA"
     gene            253..1308
                     /gene="nuoH"
                     /locus_tag="VF453_07035"
     CDS             253..1308
                     /gene="nuoH"
                     /locus_tag="VF453_07035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX7687441.1"
                     /translation="MIEQFNQWGLAHLGGIAWPVVWALIRIVVTILPLLGCVAYLTLW
                     ERVAIGRSQIRPGPNRVGPRGLLTPIADAMKLIFKEIIRPTAANKALFLIAPIMTIMP
                     ALAAWAVMPFNPTLVIADVNAGLLFVMAITSLEVYGVIIAGWASNSKYAFLGALRASA
                     QMVSYEIAMGFCLVIVLMVSGTMNIGGIVAGQGKGMFAAHGWNFLSWNWLPLLPIFFI
                     YFISGLAETNRHPFDVVEGESEIVAGHMVEYSGMAFAMFFLAEYANMILVSALCVIMF
                     LGGWISPVGFIPDSFWWLFLKTFCVVSMFLWVRSTFPRYRYDQLMRLGWKIFIPVTLV
                     WLVVVGLWMQSPLSIWK"
     gene            1334..1852
                     /gene="nuoI"
                     /locus_tag="VF453_07040"
     CDS             1334..1852
                     /gene="nuoI"
                     /locus_tag="VF453_07040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX7687442.1"
                     /translation="MTTTTERGFSRFSLKDFFSSFLLLELWKGMKLTGKYFFSPTITI
                     QFPEEKTPLSPRFRGLHALRRYDNGEERCIACKLCEAVCPAMAITIESDVRSDGSRRT
                     TRYDIDLTKCIFCGFCEESCPVDSIVETHILEYHGEKRGDLYFTKDMLLAVGDRYERE
                     IAAAKEADAKYR"
     gene            1893..2552
                     /locus_tag="VF453_07045"
     CDS             1893..2552
                     /locus_tag="VF453_07045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX7687443.1"
                     /translation="MTTTSVLFYIFSAVLLASSFGVITARSTVHAALFLVLAFFNASC
                     LWMMLKAEFLAITLVLVYVGAVMVLFLFVVMMLDINTEELRAGFWRAMPVAGAVGALV
                     ALEMALVLMGGFRRAEATPFDAGLMKQGNTHALGFEVYTNYLYPLEIAAVLLLVAIVS
                     AIALTLRQRKDTKTMDPSLQVRARKADRLRIVKMAPVVDLPSAPPAAPEAAAKTEGGK
                     Q"
     gene            2613..2930
                     /gene="nuoK"
                     /locus_tag="VF453_07050"
     CDS             2613..2930
                     /gene="nuoK"
                     /locus_tag="VF453_07050"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX7687444.1"
                     /translation="MNVGDITLAHYLTLGGMLFALSVVGIFLNRRNLIVLLMAIELML
                     LAVNLNFVAFSHYLGDMAGQVFVFFILTVAAAESAIGLAILVVLFRNRATIAADELDE
                     LKG"
     gene            3035..5140
                     /gene="nuoL"
                     /locus_tag="VF453_07055"
     CDS             3035..5140
                     /gene="nuoL"
                     /locus_tag="VF453_07055"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7687445.1"
                     /translation="MSANLSLSQLLIVPLAPLVGAIGAGLFGKQIGRKGAHTLTILGV
                     LVSFIVSAMTLYSVAVDGARFAGTVYEWMNAGGLKMEVGFQIDGLTAMMMCVVTFVSL
                     MVHLYTVGYMEEDPGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIG
                     FWFKRPTAIFANLKAFLVNRVGDFGFILGIGLIAAYTHSLNYAEVFDAFAKKPELAAT
                     LLPHSDWLLITVICICLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGI
                     FMVTRMSALFELSDTALNFVLVIGSITALFMGFLGIIQNDIKRVVAYSTLSQLGYMTV
                     ALGLSAYHVAVFHLMTHAFFKALLFLGAGSVIMGMHHDQDIRNMGGLRKYMPITWITS
                     LVGSLALIGTPFFSGFYSKDSIIEAAHHSTLPGAGFAIFSVTAGVFVTAFYSFRMYFL
                     VFHGPERFRNPPFPMPDAHHAASDPHASDVADVGAHSEPDAHDHDDHGHHHEPHESPW
                     VVTVPLVLLAIPSAVIGAMAIKPLLFGDFFKGAITVDAAKHPAMEELSKDFDGWLSAG
                     LHAFASVPFGLALLGVVLSYVMCIAKPSLSDAAEKFFRSIGVYQILENKYFFDWFNEN
                     VIAAGARLLGRGLWKGGDQGVIDGLAVNGSARVVGVFAGIVRLVQTGHLYWYALVMIL
                     GVVGLLTWQLLPYFRTLVH"
     gene            5163..6638
                     /locus_tag="VF453_07060"
     CDS             5163..6638
                     /locus_tag="VF453_07060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX7687446.1"
                     /translation="MNLLSLAIWLPIAAGVVLLAIGSDKNAPLVRWVALLAAIASFVV
                     TIPLYTGFDATTAAMQFQVNLPWIERFHAAYHLGVDGISLWFVLLTAFITVIVVIAGW
                     EVITERVNQYMGAFLILSGLMVGVFTAVDGLLFYTFFEATLIPMYIIIGVWGGPRRIY
                     ASFKFFLYTLLGSLLMLVAIVYLYFKSGGSFDILAWHALKLPMQAQTLLFFAFLAAFS
                     VKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGFVRFSLPIAPDASHEWAWFI
                     IALSLIAVIYIGLVAMVQSDMKKLVAYSSIAHMGFCTLGFFVFDSLGMQGALVQMISH
                     GFVSGAMFLCIGVLYDRVHSREIKAYGGVVNKMPWFVTFAVLFAMANCGLPGTAGFVG
                     EWMVILSSVGFNFWIGALASTALIFGAAYTLWMVKRVYFGEVANHHVAELTDINAREF
                     FMLAVLAVAVLAMGLDPKPFTDVMSVSVDQLLKHVAQSKLI"
     gene            6658..8136
                     /gene="nuoN"
                     /locus_tag="VF453_07065"
     CDS             6658..8136
                     /gene="nuoN"
                     /locus_tag="VF453_07065"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX7687447.1"
                     /translation="MTQLNLPIYPEIFLLVLTSVVALMGANASTARLNAAFWLAEISL
                     LVFSGMHLHELQSGQAIYGMQRMVVEDPLGHLLALSAGLATAITLGYAKPYLASRDLF
                     KGEFLSLTMFSLLGISVMCAANNLLVVYVGLELMTLSLYALTALRRDHAVSTEAAMKY
                     FVLGALASGFLLYGLSMMYGATGTLDIPYIFDAIHKGTIAPQVLTFGLVFIVAGLAFK
                     LGLAPFHMWVPDVYQGAPTAVTLLISGAPKLAAFAISFRILVEGLSGLGTDWQQMLIV
                     LAVVSMSLGNLAAIVQSNLKRLLAYSAIAQLGFMLLGLTPTVVNLDTAAAANGYSSAM
                     FYIITYVLTTLGTFGLVMYLSRQGFESEEVSDLAGLAKRSPWIAGVMSLFMFSLAGIP
                     PLVGFYAKLAVLQALIATGAKGYIILAVVAVLLSLIGAYYYLRVVKTMFFDEPTDATP
                     IRGETGTTLLLALNGIAALAFGLMPDGLMSLCRDAVQKALVS"
     gene            8136..8795
                     /locus_tag="VF453_07070"
     CDS             8136..8795
                     /locus_tag="VF453_07070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7687448.1"
                     /translation="MSAEQGGASGSGAEAIVAGGSAPAAGPGIDEHLVERRLRSEAVY
                     DGRFLKVRRDRVRLPGGGEAEREYIVHPGAVMIVPIADDGRLVVERQHRYPPDVVLLE
                     FPAGKLDPHETSLECARRELLEETGYAAREWAYAGVMHNAPAYSDETIEIWFARGLAA
                     GAQQLDEGEFVEVLLMDEPALFALAARGELTDAKTMVALLRLQQWRAGAWAPDWTSAP
                     A"
     gene            8819..>8967
                     /locus_tag="VF453_07075"
     CDS             8819..>8967
                     /locus_tag="VF453_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX7687449.1"
                     /translation="MKVLDLRCAAGHGFEGWFASEDDYGSQRERGLLDCPVCGNADIA
                     RLPSAP"
BASE COUNT         1285 a         3069 c         2903 g         1710 t
ORIGIN      
        1 tgaccgccga cgcgcgcgcg ccgcaggcga gcctgccgca ggggctgtgg gattcgctgg
       61 gcctggccgc tggcgacaag gtgcgcgtga cgcagggcga gggcagcgtc gtgctggccg
      121 cgcgcctgga caagacgctc gcgccgaccg ccgtgcgcgt gccggccggc cacgtgctga
      181 ccgccgccct gggcgccgcg ttcggtccga tcacggtcgc caaggcctga accgcacgag
      241 ctgagacacc aaatgatcga acaattcaat caatggggcc tcgcccacct gggcggcatc
      301 gcgtggccgg tggtctgggc gctgatccgg atcgtcgtca cgatcctgcc gctgctcggc
      361 tgcgtcgcct acctgacgct gtgggagcgc gtggccatcg gccgttcgca gatccgcccg
      421 ggcccgaacc gcgtcgggcc gcgcggtctg ctcacgccga tcgcggacgc catgaagctg
      481 atcttcaagg agatcatccg cccgaccgcc gcgaacaagg cgctgttcct gatcgccccg
      541 atcatgacca tcatgccggc gctcgcggcc tgggcggtga tgccgttcaa cccgacgctg
      601 gtgatcgccg acgtcaacgc cggcctgctg ttcgtgatgg ccatcacctc gctcgaggtg
      661 tacggcgtga tcatcgcggg ctgggcctcg aactcgaagt acgcgttcct cggcgcgctg
      721 cgcgcctcgg cccagatggt cagctacgag atcgcgatgg gcttctgcct cgtgatcgtg
      781 ctgatggtct cgggcacgat gaacatcggc gggatcgtcg cggggcaggg caagggcatg
      841 ttcgcggcac acggctggaa tttcctgtcg tggaactggc tgccgctgct gcccatcttc
      901 ttcatctact tcatctcggg cctcgcggaa accaaccgcc acccgttcga cgtggtggaa
      961 ggcgagtcgg agatcgtggc gggccacatg gtcgagtact cgggcatggc gttcgcgatg
     1021 ttcttcctcg ccgaatacgc caacatgatc ctggtctcgg cgctgtgcgt gatcatgttc
     1081 ctgggcggct ggatcagccc ggtcggcttc atccccgaca gcttctggtg gctgttcctg
     1141 aagacgttct gcgtcgtctc gatgttcctg tgggtgcgct cgaccttccc gcgctaccgc
     1201 tacgaccagc tgatgcgcct cggctggaag atcttcatcc ccgtcacgct ggtgtggctg
     1261 gtggtcgtcg gcctgtggat gcagtccccg ctgtcgatct ggaagtgaag cgaggacgcc
     1321 aaccatgacc accatgacca cgacgaccga acgcggcttc tcccgctttt cgctcaagga
     1381 cttcttctcc agcttcctcc tgctcgagct ctggaagggg atgaagctga ccggcaagta
     1441 cttcttctcg ccgacgatca ccatccagtt cccggaagag aagaccccgc tcagcccgcg
     1501 cttccgcggc ctgcacgcgc tgcgccgcta cgacaacggc gaggagcgct gcatcgcgtg
     1561 caagctgtgc gaggcggtgt gcccggcgat ggcgatcacc atcgagtccg acgtgcgctc
     1621 cgacggctcg cgccgcacga cgcgctacga catcgacctg accaagtgca tcttctgcgg
     1681 cttctgcgag gaaagctgcc cggtcgactc gatcgtcgag acgcacatcc tcgagtacca
     1741 cggcgagaag cggggcgacc tgtacttcac caaggacatg ctgctcgcgg tcggtgaccg
     1801 ctacgagcgc gagatcgcag ccgccaagga ggccgacgcc aagtaccgct gaagcgcgac
     1861 cccgcgcgag gcgtacccgg taccgtattg ccatgaccac tacctccgtc ctgttctaca
     1921 tcttctcggc cgtgctgctg gcgtcctcgt tcggtgtcat caccgcgcgt tcgaccgtgc
     1981 atgccgcgct gttcctggtg ctcgccttct tcaacgcatc ctgcctgtgg atgatgctga
     2041 aggccgagtt cctcgcgatc accctggtgc tcgtgtacgt cggcgccgtg atggtgctgt
     2101 tcctgttcgt cgtgatgatg ctcgacatca acacggagga actgcgcgcc ggcttctggc
     2161 gcgccatgcc cgtggcgggc gccgtgggcg cgctggtcgc tctcgagatg gcgctcgtgc
     2221 tgatgggcgg cttccgccgc gccgaggcca cgccgttcga cgccgggctg atgaagcagg
     2281 gcaacacgca cgcgctcggc ttcgaggtct acacgaacta cctgtacccg ctggagatcg
     2341 cggcggtgct gctgctggtg gccatcgtct cggccatcgc gctgacgctg cgccagcgca
     2401 aggacaccaa gaccatggat ccctcgctgc aggtgcgcgc ccgcaaggcc gaccgcctgc
     2461 gcatcgtcaa gatggcgccg gtcgtcgacc tgccgtcggc gccgcccgcc gcgccggagg
     2521 ctgccgccaa gaccgaagga ggcaagcaat gagctcccgc tcggccgctc cgcacggagc
     2581 ccgcaccgca gtccgcagga cggaggtttc aaatgaacgt cggtgacatc acgctcgccc
     2641 actacctgac gctgggcggc atgctgttcg cgctgtcggt cgtcggcatc ttcctgaacc
     2701 gccgcaacct gatcgtgctg ctgatggcca tcgagctgat gctgctggcg gtcaacctga
     2761 acttcgtcgc cttctcgcac tacctgggcg acatggccgg ccaggtcttc gtgttcttca
     2821 tcctgaccgt cgccgcggcc gagtccgcga tcggcctcgc gatcctggtc gtgctgttcc
     2881 gcaatcgcgc gacgattgcc gcggacgagc tcgacgagct gaagggctag acatgacgac
     2941 tcccattcgc tccgctcatc gtcccccgac gggactgggc cgctcgctcc ggagcggccg
     3001 agcgctcgcg gcctgacgga aagaaaaaaa acagatgtct gcgaacctgt ctctctccca
     3061 actcctgatc gtgccgctgg cgccgctggt gggcgcgatc ggtgctggcc ttttcggcaa
     3121 gcaaatcggc cgcaagggcg cgcatacgct gaccatcctc ggcgtcctgg tctcgttcat
     3181 cgtctcggcg atgacgctgt actcggtcgc ggtcgacggc gcgcgcttcg cgggcaccgt
     3241 ctacgagtgg atgaacgccg gcggcctgaa gatggaagtc ggcttccaga tcgacggcct
     3301 gaccgcgatg atgatgtgcg tcgtgacctt cgtgtctctg atggtccacc tctacaccgt
     3361 gggctacatg gaggaggatc cgggctacca gcgcttcttc tcgtacatct cgctgttcac
     3421 cttctcgatg ctcatgctgg tcatgagcaa caacttcctg cagctgttct tcggctggga
     3481 agcggtgggc ctcgtctcgt acctgctgat cggcttctgg ttcaagcggc cgacggccat
     3541 cttcgccaac ctcaaggcct tcctggtcaa ccgcgtcggc gacttcggct tcatcctcgg
     3601 catcggcctg atcgccgcct acacgcactc cctgaactac gccgaggtct tcgacgcctt
     3661 cgcgaagaag ccggagctcg ccgcaacgct gttgccgcac agcgactggc tgctgatcac
     3721 cgtgatctgc atctgcctgt tcatcggtgc catgggcaag agcgcgcagt tcccgctgca
     3781 cgtctggctg cctgattcca tggaaggccc gacgccgatc tcggcgctga tccacgcagc
     3841 gacgatggtc acggccggca tcttcatggt cacgcgcatg tcggccctct tcgagctgtc
     3901 ggacacggcg ctgaacttcg tgctggtgat cggctcgatc acggcgctgt tcatgggctt
     3961 cctgggcatc atccagaacg acatcaagcg cgtcgtcgcg tactcgacgc tctcgcagct
     4021 gggctacatg accgtggcgc tgggcctgtc ggcgtaccac gtcgccgtct tccacctgat
     4081 gacgcacgcg ttcttcaagg cgctgctgtt cctcggcgcg ggctcggtca tcatgggcat
     4141 gcaccacgac caggacatcc gcaacatggg cggcctgcgc aagtacatgc ccatcacctg
     4201 gatcacgtcg ctggtcggtt cgctggcgct gatcggcacc ccgttcttct cgggcttcta
     4261 ctcgaaggac tcgatcatcg aggcggcgca ccacagcacg ctgccgggcg cgggcttcgc
     4321 gatcttctcg gtgacggcgg gcgtcttcgt gacggcgttc tactcgttcc ggatgtactt
     4381 cctggtcttc catggcccgg aacgcttccg caacccgccg ttcccgatgc ccgacgcgca
     4441 ccacgccgca agcgacccgc acgcgagcga cgtcgccgac gtgggcgcgc attcggagcc
     4501 cgacgcccac gaccacgacg accacggcca ccaccacgag ccgcacgagt cgccgtgggt
     4561 cgtgacggtg ccgctggtgc tgctggcgat cccgtcggcc gtcatcggcg cgatggcgat
     4621 caagccgctg ctgttcggcg acttcttcaa gggcgcgatc acggtagacg ccgcgaagca
     4681 cccggcgatg gaagagctca gcaaggattt cgacggctgg ctgtcggcgg gcctgcatgc
     4741 gttcgcctcg gtgccgttcg ggctcgcgct gctgggcgtc gtgctgtcgt acgtgatgtg
     4801 catcgccaag ccgtcgctgt cggatgccgc ggagaagttc ttccgctcca tcggcgtcta
     4861 ccagatcctc gagaacaagt acttcttcga ctggttcaac gagaacgtca tcgccgccgg
     4921 cgcgcgcctg ctgggccgcg gcctgtggaa gggtggcgac caaggcgtca tcgacggcct
     4981 ggcggtcaac ggctccgcgc gtgtggtcgg cgtcttcgcc ggcatcgtgc gcctggtcca
     5041 gaccggccac ctctactggt atgcgctggt gatgatcctc ggcgtcgtcg gcctgctgac
     5101 ctggcagctg ctgccttact tccggacgct cgtccactga caagccggcg gagaacacaa
     5161 gaatgaatct tctgagtctg gccatctggc tgcccatcgc ggcaggggtg gtgctgctgg
     5221 cgatcggcag cgacaagaac gcgccgctgg tccgctgggt ggcgctgctg gcggcgatcg
     5281 cgtccttcgt ggtcacgatc ccgctgtaca cgggcttcga cgcaacgacc gcggcgatgc
     5341 agttccaggt caacctgccc tggatcgagc gcttccacgc cgcctaccac ctcggtgtgg
     5401 acggcatctc gctgtggttc gtgctgctca cggccttcat caccgtcatc gtggtgatcg
     5461 ccggctggga ggtcatcacc gagcgcgtga accagtacat gggcgcgttc ctgatcctct
     5521 cgggcctcat ggtgggcgtg ttcacggcgg tcgacggcct gctgttctac acgttcttcg
     5581 aggcgaccct gatcccgatg tacatcatca tcggcgtctg gggcggcccg cggcggatct
     5641 acgcgtcgtt caagttcttc ctgtacacgc tgctcggctc gctgctgatg ctggtcgcga
     5701 tcgtgtacct ctacttcaag tcgggcggca gcttcgacat cctcgcctgg catgcgctga
     5761 agctgccgat gcaggcgcag acgctgctgt tcttcgcgtt cctggccgcg ttctcggtca
     5821 aggtgccgat gtggccggtg cacacctggc tgccggatgc ccacgtcgag gcgcccacgg
     5881 gcggctccgt cgtgctggcg gcgatcatgc tgaagctggg cgcgtacggc ttcgtgcgct
     5941 tctcgctgcc gatcgcgccg gacgcgagcc acgagtgggc ctggttcatc atcgcgctgt
     6001 cgctgatcgc ggtcatctac atcggcctcg tggcgatggt gcagagtgac atgaagaagc
     6061 tggtcgccta ctcgtcgatc gcgcacatgg gcttctgcac gctcggcttc ttcgtcttcg
     6121 actcgctggg catgcaaggg gcgctcgtgc agatgatctc gcacggcttc gtctccggcg
     6181 cgatgttcct gtgcatcggc gtgctctacg accgggtcca ctcgcgcgag atcaaggcct
     6241 acggcggcgt cgtcaacaag atgccgtggt tcgtcacctt cgcggtgctg ttcgcgatgg
     6301 ccaactgcgg cctgccgggc accgccggct tcgtgggcga gtggatggtc atcctgtcga
     6361 gcgtcggctt caacttctgg atcggcgcgc tcgcctcgac cgccctgatc ttcggcgcgg
     6421 cctacacgct gtggatggtc aagcgggtgt acttcggcga ggtcgccaac caccacgtcg
     6481 ccgagctgac cgacatcaac gcccgcgagt tcttcatgct cgccgtgctc gccgtcgcgg
     6541 tgctggcgat gggcctggat cccaagcctt tcaccgacgt gatgagcgtc tcggtggacc
     6601 agctgctgaa gcacgtcgcc cagtccaagc tgatctgagc gccgcggaaa gactcccatg
     6661 acccaattga acctgccgat ctatcccgag atcttcctgc tggtgctgac ctcggtggtc
     6721 gcgttgatgg gcgccaacgc ctcgacggcg cgcctgaacg ccgcgttctg gctggccgag
     6781 atctcgctgc tcgtcttctc gggcatgcac ctgcacgagc tgcagtccgg ccaggccatc
     6841 tacggcatgc agcgcatggt cgtcgaggat ccgctgggcc acctgctggc gctgtcggcc
     6901 ggcctggcca cggcgatcac gctcggctat gccaagccct acctggcctc gcgcgacctg
     6961 ttcaagggcg agttcctgtc gctgacgatg ttctcgctgc tgggcatctc ggtgatgtgc
     7021 gccgccaaca acctgctggt ggtctacgtg ggcctcgagc tgatgacgct gtcgctgtac
     7081 gcgctcaccg cgctacgccg cgaccatgcc gtctcgaccg aggcggcgat gaagtacttc
     7141 gtcctcggcg cgctggccag cggcttcctg ctgtacggcc tgtcgatgat gtacggcgcc
     7201 accggcacgc tcgacatccc ctacatcttc gacgccatcc acaagggcac catcgcgccg
     7261 caggtcctga ccttcggcct cgtgttcatc gtcgccggcc tcgcgttcaa gctcggcctc
     7321 gcgccgttcc acatgtgggt gcccgacgtc taccagggcg cgccgaccgc ggtcacgctg
     7381 ctgatctcgg gcgcaccgaa gctggccgcg ttcgcgatca gcttccgcat cctggtggaa
     7441 ggcctgtcgg gcctgggcac cgactggcag cagatgctga tcgtgctggc cgtcgtctcg
     7501 atgtcgctgg gtaacctggc ggccatcgtg cagtcgaacc tgaagcgcct gctggcgtac
     7561 tcggcgatcg cgcagctggg cttcatgctg ctggggctga cgccgaccgt cgtgaacctg
     7621 gacaccgccg ccgccgccaa cggctacagc tcggcgatgt tctacatcat cacctacgtg
     7681 ctgacgacgc tgggcacctt cggcctggtg atgtacctgt cgcgccaggg cttcgagagc
     7741 gaggaagtct ctgatctcgc cggcctggcc aagcgctcgc cctggatcgc gggcgtgatg
     7801 tcgctgttca tgttctcgct cgccggcatc ccgccgctgg tcggcttcta tgccaagctc
     7861 gccgtgctgc aggcgctgat cgccaccggc gcgaagggct acatcatcct cgcggtggtc
     7921 gcggtgctgc tgtcgctgat cggtgcctac tactacctgc gcgtcgtcaa gacgatgttc
     7981 ttcgacgagc cgaccgatgc gacgccgatc cgcggcgaga ccggcaccac gttgctgctg
     8041 gcgctcaacg gcatcgccgc gctggccttc gggctgatgc cggacgggct gatgtcgctg
     8101 tgccgcgacg ccgtgcagaa ggcgctcgtc agctgatgtc cgcggagcag ggcggcgcga
     8161 gcggatccgg ggccgaggcg atcgtcgccg gcggctccgc cccggccgcc ggcccgggca
     8221 tcgacgagca tctcgtcgag cgccgcctgc gctccgaggc cgtgtacgac ggccgcttcc
     8281 tgaaggtgcg ccgcgaccgc gtgcgcctgc ccggcggcgg cgaggccgag cgcgagtaca
     8341 tcgtgcatcc gggcgcggtg atgatcgtgc cgatcgcgga cgacggccgc ttggtcgtgg
     8401 aacgccagca ccgctatccg cccgacgtcg tgctgctgga attccccgcc ggcaagctcg
     8461 atccgcacga gacctcgctc gagtgcgcgc gccgggaact gctcgaggag accggctacg
     8521 ccgcccgcga gtgggcctat gcgggggtga tgcacaacgc gccggcctat tcggacgaga
     8581 cgatcgagat ctggttcgcg cgcggcctcg cggccggggc gcagcaactg gacgagggcg
     8641 agttcgtcga ggtgctgctg atggatgagc cggccctgtt cgcgctcgcc gcgcgcggcg
     8701 agctcaccga tgccaagacg atggtcgcgc tgctgcgcct gcagcagtgg cgcgccgggg
     8761 cgtgggcgcc ggactggacg tccgcgcccg cctgatcagc accgcacgac cccttgccat
     8821 gaaggtcctc gacctgcgct gcgctgccgg ccacggcttc gaaggctggt tcgcctccga
     8881 ggacgactac ggctcgcagc gcgagcgcgg cctgctcgat tgccccgtct gcggcaacgc
     8941 ggacatcgcg cggctgccca gcgcgcc
//
LOCUS       DASVLN010000290         7087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_499922, whole genome shotgun sequence.
ACCESSION   DASVLN010000290 DASVLN010000000
VERSION     DASVLN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499922"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VF453_07080"
     CDS             complement(<1..431)
                     /locus_tag="VF453_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX7687450.1"
                     /translation="MGEMVNNGGSSAAGIDLSQFYQVFFEEAGENLEQMEQKLLELNV
                     DAADDEELNAIFRCAHSVKGGAATFGFSDVAALTHQMETLLDKLRRHELAPTSAMVDV
                     LLAAGDALKAQLAVHQGSATSAPDTSELLFNIRAMAEGTAPA"
     gene            complement(490..852)
                     /locus_tag="VF453_07085"
     CDS             complement(490..852)
                     /locus_tag="VF453_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7687451.1"
                     /translation="MHTILAVDDSPSMRQMVAFTLKNAGFNVVEAVDGQDAFEKSTQR
                     DYNLVLTDQNMPRMDGISLTKKLRENPKFKQTPILILTTESSDQMKMAGRAAGATGWL
                     VKPFDPAKLIEVIGKVVR"
     gene            complement(900..1358)
                     /locus_tag="VF453_07090"
     CDS             complement(900..1358)
                     /locus_tag="VF453_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687452.1"
                     /translation="MLTSDAQNPSPGGGLPLVAAADLQDHLMVASHDLERLQRLLEDA
                     GEALMGHFYGASNQMNVLRDEAPKFNLDTAPIKEALQSLAGAITALQFQDMATQLIAH
                     TQRRLRNCADRIARDAMGDDEDGAAVVEDAPMRPNPVTQDEMDAGSIDLF"
     gene            1652..2836
                     /locus_tag="VF453_07095"
     CDS             1652..2836
                     /locus_tag="VF453_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7687453.1"
                     /translation="MPSHCLPARRTALRVLHLEDSELDHELEMAHLRRGGLRLEATRV
                     DSEAAFRAALDEPVAWDIVLSDFNVPGFSGLAALEIVKATGRPLPFILISGEIGEDVA
                     VAAMRNGASDYLLKANLARLLPAVEHAIAANEAELAREAADRELALSRQRLSELAQHL
                     QTSVDMERAAIAREIHDDVGGSLTALKFDIAWIARHAGDPDVQQRAQAALEVVTHAIE
                     SSKRIMHNLHPAILEQGLVAALQWMASRFEKRTGVVCAIRTPSEALQLPAGVPLVAYR
                     TAQEALTNITKHANATRVGIDLSLAGGVLSLEISDNGVGLSDADLAKARSFGIRGLHE
                     RASTVGGWVDLSGSPGQGTTLILSVPLADAVGYASEFGPDAVDDDEAAGDDDDPTVWG
                     AP"
     gene            2833..3471
                     /locus_tag="VF453_07100"
     CDS             2833..3471
                     /locus_tag="VF453_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7687454.1"
                     /translation="MIRVILCDDHALIRRGIRDTLSDAPDIEVVGEAGDYPELRSLLR
                     TTTCDVLVLDINMPGRSGLDVLAVLKDEGATMKVLIVSMYPEDQYAIRALRAGAYGYV
                     NKGGDPQQLVLAVRTVSQGRKFVTPEIAQMLVESLTTPEVAHAHEKLSDRELQTLVMI
                     ASGKRLSDIAGELTLSPKTVSVYRARVLEKLGLANNSELTVYAIRNGLVGGE"
     gene            complement(3642..4274)
                     /locus_tag="VF453_07105"
     CDS             complement(3642..4274)
                     /locus_tag="VF453_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEX7687455.1"
                     /translation="MKMEDIASLKPTDTLSVSPEVFQQLGTITRLLHDTMQQLGVMPK
                     LQLATDGLPDARSRLTYIATKTAAAAEKVLNSVDQAKAEHNSISEATRAIAAQIVSDP
                     VKAVATGAVMNFVHEVETSTKKIDEHLTDIMMAQDFHDLTGQVVAKVVTLANDLEDSL
                     VKLLVQVVPEGVRAQVDPNVLNGPVVNPEGRTDVVADQGEVDDLLASLGF"
     gene            complement(4292..4690)
                     /gene="cheY"
                     /locus_tag="VF453_07110"
     CDS             complement(4292..4690)
                     /gene="cheY"
                     /locus_tag="VF453_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HEX7687456.1"
                     /translation="MSNPTDLKFLVVDDFSTMRRIVRGLLKEMGCNNCEEAEDGQVAL
                     NMLKAAKFDFVVSDINMPNMNGFDLLKSIKADDSLKHLPVLMVTAEARKEDIVLAAQS
                     GAAGYIVKPFTKATLEEKVQKIMQKLAATA"
     gene            complement(4739..5707)
                     /gene="motB"
                     /locus_tag="VF453_07115"
     CDS             complement(4739..5707)
                     /gene="motB"
                     /locus_tag="VF453_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX7687457.1"
                     /translation="MAGDSKKLQPIIIKRVKKGGHAAHGGAWKIAYADFVTAMMAFFL
                     LMWLLGSTTDGDKKGIADYFASPLKIALSGGSGSGDSNSVLKGGGESLTSTVGQVKKG
                     EVEAQRNTINLHKLKAEQVRAEVSRLEDLKQEIQQKLQSNEKLKDLSSQIRLDMTRDG
                     LRIQIVDDQNRPMFDSGSAVIKPYMRDLLRAIGSVLTEVPNHLTLEGHTDAQPFPGGD
                     RGYSNWELSADRANASRRELIAGGLADDRVLRVQGLASSQPFDEKDPTAPANRRISII
                     VMNRDAEDRMFRPLDASAAEAEESPASAATNSTVSPPADASSAAAH"
     gene            complement(5725..6585)
                     /gene="motA"
                     /locus_tag="VF453_07120"
     CDS             complement(5725..6585)
                     /gene="motA"
                     /locus_tag="VF453_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEX7687458.1"
                     /translation="MFVIVGWVVALGCIFGVYIAEEGNMHVILHALPFEFITIFGAAG
                     GAFLANNQMKVVKAAAKGLGACFKGSKFSKARYMELLALLYDILQKARKEGLMSIEKD
                     VEDPHSSALFQKYPTVGGDHHVVEFITDYLRMMVSGNLNAHEIESLMDSEIETHHHEE
                     HAAVAALGRLAGGLPAFGIVAAVLGVVKTMGSVGQPPAVLGAMIGSALVGTFLGILLA
                     YGFVEPLGGLLEQKVEDNGKELQCIKTTLLASMQGYAPQVAIEFGRKVLYSGDRPTFV
                     ELESHVKGKK"
     gene            complement(6752..>7087)
                     /locus_tag="VF453_07125"
     CDS             complement(6752..>7087)
                     /locus_tag="VF453_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7687459.1"
                     /translation="LRALRAVWPQARLEGIEWSWPLALAARLRCPWAGVRRGDMWRPG
                     AWSGCALVYLFQRPESMARAIAKAEAELPSGAWLASLEFEVAGRRPHAVLRPAGGKPV
                     WIYRVGDAR"
BASE COUNT         1018 a         2390 c         2452 g         1227 t
ORIGIN      
        1 gcgggcgcgg tgccctcggc catcgcgcgg atgttgaaca gcagctcgct cgtgtccggc
       61 gcggacgtcg ccgagccctg gtgcaccgcg agctgcgcct tgagcgcgtc gccggcggcc
      121 agcagcacgt cgaccatcgc cgacgtcggc gccagctcgt ggcggcgcag cttgtccagc
      181 agggtctcca tctggtgcgt cagcgcggcg acgtcggaga agccgaacgt cgcggcgccg
      241 cccttgacgg agtgcgcgca gcggaagatc gcgttcagct cctcgtcgtc ggcggcgtcg
      301 acgttgagct ccaggagctt ctgctccatc tgctcgaggt tctcgccggc ttcctcgaag
      361 aagacctggt agaactgact cagatcgatc ccggcggccg aactgccgcc attgttgacc
      421 atctcaccca tgactctttt ctcctgatcc ggtgtttctt gccgtcccca cgcgggcggg
      481 gacgcggctt cagcggacga ccttgccgat gacctcgatc agcttggcgg ggtcgaaggg
      541 cttgacgagc cagccggtgg caccggccgc gcggccggcc atcttcatct gatcgctcga
      601 ctcggtggtc aggatgagga tcggcgtctg cttgaacttg gggttctcgc gcagcttctt
      661 cgtcaggctg atgccgtcca tgcgaggcat gttctggtcg gtgagcacga ggttgtagtc
      721 gcgctgggtc gatttctcga acgcgtcctg gccatccacg gcctcgacga cgttgaagcc
      781 ggcgttcttc agggtgaacg ccaccatctg gcgcatcgag ggggaatcgt ccacggcgag
      841 gatcgtgtgc atttgcagtt ctccggaaat cttgggggta tgggctggaa gggaggaagt
      901 cagaacaggt cgatggagcc ggcgtccatt tcgtcctgcg tcaccggatt cgggcgcatg
      961 ggggcgtcct cgacgacggc cgcgccgtcc tcgtcgtccc ccatcgcgtc gcgcgcgatc
     1021 cggtcggcgc agttgcgaag ccgtcgctgc gtgtgcgcga tcagctgggt ggccatgtcc
     1081 tggaactgca gggcggtgat ggcaccggcc aggctctgga gcgcttcctt gatcggcgcc
     1141 gtgtccaggt tgaatttcgg ggcttcgtcg cgcagcacgt tcatctggtt ggacgcgccg
     1201 tagaagtggc ccatcaaggc ctcgcccgcg tcctcgagca gccgctgcag ccgttcgagg
     1261 tcgtgcgagg cgaccatcag gtgatcctgc aggtctgccg ccgccacgag cgggagtccc
     1321 ccgccgggcg atggattctg ggcgtcactc gtcagcatcg tggctccaaa agctgtgtgt
     1381 tgtgttgtgc gcgggaggcg ttgcggttgc ccgccgcgga ttccctcgag gattgcgctt
     1441 gattcttcgg cacgcgaccc tcggacattg agcggatcac ggtggaaaac gcccgccatt
     1501 tctcgatctg gcgccaagcg ggcgcgaccc gtgcgattcc tcacgccagg gcggcgcgcg
     1561 gcgggcgcct gccccgtgat ttcggctcga aggcgcgcgg acctgagggc cgtccgggct
     1621 tgcggcgcct cggctgggcg atactccacg catgccttcc cactgcctgc cggcccgacg
     1681 cacagcgctg cgcgtgctgc acctggagga ttcggagctc gaccacgagc tcgagatggc
     1741 gcacctgcgc cgcggcggcc tgcggctgga ggcgacccgc gtcgactccg aggccgcctt
     1801 ccgcgccgcg ctggacgagc cggtggcctg ggacatcgtc ctgtcggact tcaacgtgcc
     1861 cggcttctcg ggcctggcgg ccctggagat cgtgaaggcg accggccgcc cgctgccctt
     1921 catcctcatc tcgggcgaga tcggcgagga cgtcgccgtc gccgcgatgc gcaacggcgc
     1981 gagcgactac ctgctcaagg ccaacctcgc gcgcctgctg ccggcggtcg agcacgcgat
     2041 cgcggccaac gaggccgagc tcgcgcgcga ggccgccgac cgcgagctcg cgctgtcgcg
     2101 ccagcgcctg tcggagctcg cccagcacct gcagaccagc gtcgacatgg agcgcgccgc
     2161 gatcgcccgc gagatccacg acgacgtcgg cggctcgctg acggcgctga agttcgacat
     2221 cgcctggatc gcgcgccacg ccggcgaccc ggatgtccag cagcgcgcgc aggccgcgct
     2281 cgaggtggtg acgcacgcga tcgagtcgag caagcgcatc atgcacaacc tgcatcctgc
     2341 gatcctggag cagggcctgg tggccgcgct gcagtggatg gccagccgct tcgagaagcg
     2401 caccggcgtc gtctgcgcga tccgcacgcc cagcgaggcg ctgcagctgc cggccggcgt
     2461 gccgctggtc gcgtaccgca ccgcgcagga ggcgctcacc aacatcacca agcacgccaa
     2521 cgcgacccgc gtcgggatcg acctgtcgct ggccggcggg gtgctctcgc tggagatcag
     2581 cgacaacggc gtcggcctca gcgacgccga cctcgccaag gcgcgcagct tcggcatccg
     2641 cgggctgcac gagcgcgcct ccacggtggg cggctgggtc gacctttccg gcagcccggg
     2701 acagggcacg acgctgatcc tgtccgtgcc gctggccgac gccgtcggct acgcttccga
     2761 attcgggccg gacgccgtcg acgacgacga ggcggccggc gacgacgacg atccgaccgt
     2821 gtggggagca ccatgatccg agtcatcctc tgcgacgacc atgcgctgat ccggcgcggc
     2881 atccgcgaca cgctgtccga tgcgcccgac atcgaggtgg tgggcgaggc cggcgactac
     2941 cccgagctgc gctcgctgct gcgcacgacg acctgcgacg tgctcgtgct ggacatcaac
     3001 atgcccggcc gcagcgggct ggacgtgctg gccgtgctca aggacgaggg cgcgacgatg
     3061 aaggtgctca tcgtcagcat gtatcccgag gatcaatacg cgatccgcgc gctgcgcgcc
     3121 ggcgcctacg gctacgtcaa caagggcggg gacccgcagc agctcgtgct ggcggtgcgc
     3181 accgtctcgc agggccgcaa gttcgtgacg cccgagatcg cgcagatgct ggtcgagagc
     3241 ctgaccacgc ccgaggtcgc gcacgcgcac gagaagctct ccgaccgcga gctgcagacg
     3301 ctcgtgatga tcgcctcggg caagcgcctg tccgacatcg cgggcgagct cacgctgtcg
     3361 cccaagacgg tcagcgtcta ccgcgcacgc gtcctcgaga agctggggct cgccaacaat
     3421 tccgagctga cggtctacgc gatccgcaac ggcctggtcg gcggcgaata gcgatcaccc
     3481 atgaaaaacg cccgcggctg cgggcgtttc atcgagctcg acccggcgct gccgctcggg
     3541 tgtgtccaac gcaacacgag ggagcggcag atccggctgc gccggtctgc ccgcggccgg
     3601 cccccctggg gggcagcgag cgccagcgag cgtgggggcc gtcagaaccc caggctggcg
     3661 agcaggtcgt cgacttcgcc ctggtcggcc accacgtcgg tgcggccttc cgggttcacg
     3721 accgggccgt tgagcacgtt cgggtcgacc tgggcgcgca cgccctcggg caccacctgg
     3781 acgagcagct tgaccaggct gtcctcgagg tcgttggcca gggtcacgac cttggcgacc
     3841 acctggccgg taaggtcgtg gaagtcctgg gccatcatga tgtcggtcag gtgctcgtcg
     3901 atcttcttcg tggaggtctc gacctcgtgc acgaagttca tcacggcacc ggtggcgacc
     3961 gccttgaccg gatcgctgac gatctgggcc gcgatcgcgc gggtggcttc cgagatcgag
     4021 ttgtgctcgg ccttggcctg gtcgaccgag ttcagcacct tctcggccgc ggccgccgtc
     4081 ttggtggcga tgtaggtcag gcggctgcgc gcgtcgggca ggccgtcggt ggccagctgg
     4141 agcttgggca tcacgcccag ctgctgcatc gtgtcgtgca gcaggcgcgt gatcgtgccc
     4201 agttgctgga acacttccgg cgacacggac agcgtgtcgg tgggcttcag ggaggcgatg
     4261 tcttccattt tcatgatcgg gtctccctgg ttcaggcggt ggccgccagc ttctgcatga
     4321 tcttctgcac cttctcttcc aaggtggcct tggtgaaggg cttgacgatg tagcccgcgg
     4381 cgccggactg ggccgccagc acgatgtctt ccttgcgcgc ctcggcggtc accatcagca
     4441 ccggcaggtg cttgagcgag tcgtccgcct tgatcgactt cagcaggtcg aagccgttca
     4501 tgttgggcat gttgatgtcc gagacgacga agtcgaactt cgcggccttg agcatgttca
     4561 gggcgacctg gccgtcctcg gcctcctcgc agttgttgca gcccatctcc ttgagcaggc
     4621 cgcggacgat gcggcgcatg gtggagaagt cgtcgacgac aaggaatttc agatcggtgg
     4681 ggttgctcat ggtgtcctca gggcagggtg ctgcgagaag tgctttcggc tatatcggtc
     4741 agtgggcggc cgcgcttgag gcgtccgcgg gaggcgaaac ggtggaattt gtcgcggcgg
     4801 aggcgggaga ttcctcggct tcggccgcgg acgcgtcgag cggcctgaac atgcggtcct
     4861 cggcgtcgcg gttcatcacg atgatgctga tgcggcggtt ggcgggcgcg gtcggatcct
     4921 tctcgtcgaa aggctggctc gacgcgaggc cctgcacgcg cagcacccgg tcgtcggcca
     4981 ggccgcccgc gatcagctcg cgccgggacg cgttcgcgcg gtcggccgac agctcccagt
     5041 tgctgtagcc gcggtcgccg ccggggaaag gctgggcgtc ggtgtggccc tcgagggtca
     5101 ggtggttggg cacctcggtc agcaccgagc cgatcgcgcg cagcaggtcg cgcatgtagg
     5161 gcttgatgac cgcggagccg gaatcgaaca tcggccggtt ctggtcgtcg acgatctgga
     5221 tgcgcaggcc gtcgcgcgtc atgtccaggc ggatctggga gctgaggtcc ttcagcttct
     5281 cgttgctctg gagcttctgc tggatctcct gcttgaggtc ctcgagccgc gagacctcgg
     5341 cgcggacctg ttcggccttg agcttgtgca ggttgatcgt gttgcgctgg gcctcgacct
     5401 cgcccttctt gacctggccg acggtggagg tgaggctctc gccgccgccc ttgagcacgg
     5461 agttggagtc gcccgagccc gagccgcccg acagcgcgat cttcagcggc gaggcgaagt
     5521 agtcggcgat gcccttcttg tcgccgtcgg tggtggagcc gagcagccac atcagcagga
     5581 agaacgccat catcgccgtc acgaagtcgg cgtaggcgat cttccacgcg ccgccgtggg
     5641 cggcatggcc acccttcttg acgcgcttga tgatgatcgg ctggagcttc ttcgagtcgc
     5701 ctgccatggc ttactttccg gatctcactt cttgcccttg acgtggctct ccagctcgac
     5761 gaaggtcggg cggtcgccgg aatacagcac cttgcggccg aactcgatgg ccacctgcgg
     5821 cgcgtagccc tgcatcgagg cgagcagcgt cgtcttgatg cactgcagct ccttgccgtt
     5881 gtcctcgacc ttctgctcca gcaggccgcc cagcggctcg acgaagccgt aggccagcag
     5941 gatgcccagg aacgtgccga ccagcgccga gccgatcatc gcgcccagca ccgccggcgg
     6001 ctggccgacc gagcccatgg tcttcaccac gcccagcacg gccgcgacga tgccgaacgc
     6061 cggcaggccg cccgccaggc gcccgagcgc cgcgacggcg gcatgctcct cgtggtggtg
     6121 ggtctcgatc tcggagtcca tcagggactc gatctcgtgc gcgttgaggt tgcccgacac
     6181 catcatccgc aggtagtcgg tgatgaactc gacgacgtgg tggtcgccgc cgaccgtcgg
     6241 gtacttctgg aacagtgcgg agctgtgcgg atcctcgacg tccttctcga tggacatcag
     6301 gccttccttg cgcgccttct gcaggatgtc gtacagcagc gcgagcagct ccatgtagcg
     6361 cgccttgctg aacttgctgc ccttgaagca cgcgcccagg cccttggccg cggccttgac
     6421 caccttcatc tggttgttgg ccaggaaggc gccgccggcc gccccgaaga tcgtgatgaa
     6481 ctcgaagggg agggcgtgca ggatgacgtg catgttgcct tcctcggcga tgtacacgcc
     6541 gaagatgcag cccagtgcaa cgacccagcc gacgatcaca aacattttgg gttcgcttcc
     6601 agtgtcttgt tcgaatgcgg gcccgctttc acaggccttg cggctttatc gacgcccgcg
     6661 cgccgcgcct tgagggcgtc atccgcacga ttggcgccgg ttcccgggcc gattccgccc
     6721 ccggcgcgtg gggagatctg gacacccgtg ctcatcgcgc gtcccccacg cggtagatcc
     6781 ataccggctt gccgccggcg ggccgcagga cggcgtgcgg gcgacgtccg gccacctcga
     6841 actccaggct cgcgagccag gcgccggagg ggagctcggc ctcggccttg gcgatcgcgc
     6901 gggccatgct ctccgggcgc tggaacaggt agaccagcgc acagcccgac catgcgcccg
     6961 ggcgccacat gtcgccccgg cggacgcctg cccacgggca gcgcagccgc gcggcgagcg
     7021 ccaggggcca gctccattcg atgccttcga gccgcgcctg cggccagacc gctcgcagcg
     7081 cccgcag
//
LOCUS       DASVLN010000291         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_445585, whole genome shotgun sequence.
ACCESSION   DASVLN010000291 DASVLN010000000
VERSION     DASVLN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445585"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF453_07130"
     CDS             <1..223
                     /locus_tag="VF453_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX7687460.1"
                     /translation="QAAARLAEVVAELLSQCEDVGTRFAEEARRMHYGEREPAAIRGQ
                     TTPAEREALADEGIEVLTLPVPVAKGSLQ"
     gene            complement(338..>1648)
                     /locus_tag="VF453_07135"
     CDS             complement(338..>1648)
                     /locus_tag="VF453_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7687461.1"
                     /translation="RALAVVRPGSADEVSAVVRLCAATPGTSLVAQGGNTGLVGGGVP
                     DDSRTQVVLSLKRLRAVRRVDAANQTITVEAGLALQEVQAAAAGAGLLFPLSLASEGS
                     CTIGGNLATNAGGTQVLRFGNARELCLGLEVVTADGQVWRQLDGLRKDNTGYDLRDLF
                     VGSEGTLGIITAATLRLYPRPRAVVTGWARCASLERAVSLMRRLRAAFDAELVGFEAM
                     NAFSLALVRRHHPALRVPLDERAGEWSVLFELASPHDEATLRAGAEDTLGRALEAGEI
                     ADVALAANEAQARALWALREAIPLAQASEGLNVKHDVGLPLSALAGFVAAADAALEAV
                     APGARHVTFGHLGDGNLHYNLQAPEGEDAAAFLARLEPAVNACVHGLAIDAGGTFSAE
                     HGIGRLKRGELGARKPPQALALMRAVKTALDPRGLLNPGVMLPD"
BASE COUNT          207 a          617 c          626 g          198 t
ORIGIN      
        1 ccaggccgcg gcacgcctgg ccgaggtggt ggcggagctg ctgtcacagt gcgaggacgt
       61 cgggacgcgc ttcgccgagg aggcgcgccg catgcattac ggcgagcgcg agcccgccgc
      121 gatccgcggc cagacgacgc ccgcggagcg cgaggccctg gccgacgagg gcatcgaggt
      181 gctgacgctg ccggtgccgg tggccaaggg gtcgctgcag tagcgccgcc cgcgccggca
      241 tgcccggcgc ctcgcgtcag ggctgctgct gcagcagctt gccgatggca gccatgtccg
      301 tgtgtacctg gagtggcgcc cgcgcgtccg ccgggcgtca atccggcagc atcacgcccg
      361 gattgagcag tcctcgaggg tcgagcgccg tcttcaccgc ccgcatcagc gcgagagcct
      421 gcggcggctt gcgcgcgccc agttcgccgc gcttcaggcg accgatgccg tgctccgcgg
      481 agaaggtgcc gcccgcgtcg atcgcgagcc cgtgcacgca ggcgttgacc gcgggctcca
      541 ggcgggcgag gaaggccgcg gcgtcctcgc cttcgggcgc ctggaggttg tagtgcaggt
      601 tgccgtcgcc gagatggccg aaggtgacgt ggcgagcgcc cggcgcgacc gcctcgagcg
      661 cggcatccgc ggccgcgacg aagccggcga gcgccgacag cggcagcccc acgtcgtgct
      721 tgacgttcag gccctcgctg gcctgggcga gcgggatcgc ctcgcgcagt gcccacagcg
      781 cgcgggcctg ggcctcgttc gccgcgaggg cgacgtccgc gatctcgccg gcctcgaggg
      841 cgcggcccag cgtgtcctcc gcgccggcgc gcagcgtggc ctcgtcgtgc ggggaggcga
      901 gctcgaacag caccgaccat tcgcccgcgc gctcgtccag gggcacgcgc agcgccgggt
      961 ggtgacgccg cacgagcgcc agcgagaagg cgttcatcgc ctcgaagccg accagctcgg
     1021 catcgaaggc cgcgcgcagc cgccgcatca gcgagacggc ccgctcgagc gacgcgcagc
     1081 gcgcccagcc cgtgaccacc gcgcgcggcc gcgggtacag ccgcagcgtc gccgccgtga
     1141 tgatgcccag cgtgccctcg ctgccgacga acaggtcgcg caggtcgtag cccgtgttgt
     1201 ccttgcgcag gccgtcgagc tgacgccaga cctggccgtc ggcggtgacg acctcgagcc
     1261 cgaggcacag ctcgcgcgcg ttgccgaagc gcagcacctg cgtgccgccg gcattggtcg
     1321 cgaggttgcc gccgatcgtg cagctgccct cggaggccag gctcagcggg aacagcagtc
     1381 cggcgccggc cgcggcggcc tgcacctcct gcagcgcgag gccggcctcg acggtgatcg
     1441 tctggttggc ggcgtcgacg cggcgcaccg cgcgcaggcg cttcaggctc agcacgacct
     1501 gcgtgcgcga gtcgtccggc accccgccgc cgaccagtcc cgtgttgccg ccctgcgcga
     1561 cgaggctcgt gcccggcgtc gcggcgcaaa ggcgcacgac ggcggagacc tcgtcggcgc
     1621 tgccgggccg cacgacggcc agcgcgcg
//
LOCUS       DASVLN010000292         6642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_499972, whole genome shotgun sequence.
ACCESSION   DASVLN010000292 DASVLN010000000
VERSION     DASVLN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499972"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VF453_07140"
     CDS             complement(<1..370)
                     /locus_tag="VF453_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7687462.1"
                     /translation="MADTDTDTGTRSALRVEVGADGIAVATMDSPGRSMNVLDDTLAE
                     PLTALVGRLEADAAIKGLVVTTGKRDFLAGADVERLAALATAQEAFDESMRFKAVLRR
                     LERAGKPVVAAIRGKCLGGGL"
     gene            complement(375..1580)
                     /locus_tag="VF453_07145"
     CDS             complement(375..1580)
                     /locus_tag="VF453_07145"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7687463.1"
                     /translation="MNDALIYDHVRTPRGKGKKDGRLHQASPAWLLRGLLQGLRDRLA
                     LDTALVDDVVLGCVTPVGEQGADIARIAVLDAGWAESAAGVTLSRFCASGLEAVNLAA
                     AKVASGFEDLVVAGGVESMSRWPMGSDGGAWFMDPRVNQALGFIPQGVSADLIATLEA
                     FSRTDLDTYARDSHHRAAAAREAGRFKRSILPVHDIAGLPLLVEDETIRPDASLEAMA
                     RLEPSFAAMGAMGFDATALRKYTTLERIAHVHHAGNASGIVDGAALMLIGSRAAGERA
                     GLAPRARIRSVAVTGSEPTIMLTGPTPACRKALAKASLAAADIDLWEINEAFAAVPMK
                     TARDLAIGLDRVNVNGGAIALGHPLGATGCIILGTLLDELERRDLALGCATLCVGGGM
                     GIATIIERV"
     gene            complement(1615..2061)
                     /locus_tag="VF453_07150"
     CDS             complement(1615..2061)
                     /locus_tag="VF453_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7687464.1"
                     /translation="MSLHAARAKAQTADEVVADHRSDDAGQLFGITELCREFDISPRA
                     IRFYEDKGLLAPRRVNGTRVYTRRDRARLQLILRAKAIGSSLAEIKHYLDLYGTHGEG
                     RAVQLKYVLDKTSKAIADLERKREHIDASLAELRLINATVRKSLGK"
     gene            complement(2090..2389)
                     /locus_tag="VF453_07155"
     CDS             complement(2090..2389)
                     /locus_tag="VF453_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX7687465.1"
                     /translation="MDLQTATTQLRAKVGDDCGLGAILKFDCGDDGAILVDARDVPNT
                     VSNDASLPADCTVSLKLATLGDLLSGELQPTTGFMLGKFKVSGDMSVALKLQRIV"
     gene            2582..3532
                     /locus_tag="VF453_07160"
     CDS             2582..3532
                     /locus_tag="VF453_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7687466.1"
                     /translation="MAIAPREPSSALPGAGPRPGYRSAFAPGLFAGQRVWVTGGGSGI
                     GRAVAHELASLGAHVILSGRSPDKLERTAAEIDEDGGRCGWRAFDIRDEDAVKAQVEA
                     EVREGGVVHGLVNNAGGQFPAPMQAISKRGFEAVVANNLTGGFLMMRELFLHSFQAHG
                     GAIVNMTADFRNGMPGMAHSGAARAGMSNLTMTAAFEWASSGVRVNAVAPGWIASSGM
                     DTYGGAMRALIPQLKRHVPLRRMGLEAEVSAAIVFLLSPAAAFVTGVTLQIDGGASLG
                     SPLFPSVDHERSVPFDGFHRAATPEVLREAGGDTDTGRGS"
     gene            3532..5142
                     /locus_tag="VF453_07165"
     CDS             3532..5142
                     /locus_tag="VF453_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX7687467.1"
                     /translation="MPALGSALDPRSPAFAANAARMAERLEEVRALERQVVAASASKR
                     AKFEARGQLLPRERVAQLLDRGSGFLELSALAGLGMHDDDGRRSVQGGGSIVGIGLVA
                     GKRVLVSASDSALKGGTVSPMGLRKALRAQQVALENRLPIVYLVESGGANLMYQSEMF
                     VEGGRSFANQARLSAAGIPQVAVVHGSSTAGGAYLPGLSDYVVLVRGRSSIYLAGPPL
                     VKAAIGEDATDEELGGAQTHAEVTGLGEYLGEDDAHAIAIARELVDKLQWDAAPAPLA
                     SSPPLFDAEELLGVVPADEREPYDAREIVARIVDASDFLEFKPGFGADTLCGHARIEG
                     RLVGLLANNGPIQPQGSNKAAQFIQLCDQTGTPLVFLQNTTGYMVGSAAERGGAIKHG
                     AKMIQAVANARVAKFTVVVGGSFGAGNYGMCGRGFDPRFIFSWPSARTAVMGGAQAAK
                     VMEIVGAGKLARAGQQADEAALRAMGEQLRQRIDRESGVLFGTARLWDDGVIDPRDTR
                     RVLGLALALAHEADTRVLRPNAFGVARF"
     gene            5167..6321
                     /locus_tag="VF453_07170"
     CDS             5167..6321
                     /locus_tag="VF453_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7687468.1"
                     /translation="MHFTPEHRALADTVTRFVEKELNPYVPQWEADEIYPAHEVFAKL
                     GKLGLLGLKYPEEYGGAGLDFSYSVTMAEALGECACGGVPMSIGVQTDMCTPALARFG
                     SDELRREFLAPSIAGELVGCLGVSEPGGGSDVAAIRTTARKDGGDYVINGTKMWITNG
                     LQADWCCLLANTGEGPVHKNKSLIVVPMDAPGITKQKIRKIGMDSSDTAQLFFDEVRV
                     PQRHLIGQEGMGFAFQMMQFQEERLWGAASSLRSLDRLIDLTIEYTRDRHAFGKSVLD
                     NQVVHFRLAELRTEVAALRALTYQACELYVSGKDVTKLASMAKLKCGRLSREVADSCL
                     QFWGGMGFTRDNPISQAWRDSRLISIGGGADEVMLGIICKLEGTLPKGSH"
     gene            6323..>6642
                     /locus_tag="VF453_07175"
     CDS             6323..>6642
                     /locus_tag="VF453_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7687469.1"
                     /translation="MAEARSVPPACETLAVSSRDRVWHVMLNRPALRNAMSLRMVEEL
                     QAVLAQAERSGEVRAIVLRGAGGHFCAGADLKDMGAARQRELAGERDAVAEANARFGA
                     LCAA"
BASE COUNT          901 a         2321 c         2457 g          963 t
ORIGIN      
        1 ccagcccgcc gcccaggcac ttgccgcgga tcgccgcgac gaccggcttg ccggcgcgct
       61 cgagccggcg cagcacggcc ttgaagcgca tcgactcgtc gaaggcctcc tgcgcggtcg
      121 cgagtgcggc caggcgctcg acgtcggcgc cggcgaggaa gtcgcgcttg ccggtcgtca
      181 cgaccagccc cttgatcgcg gcgtccgcct ccaggcgccc gaccagcgcc gtcagcggct
      241 cggcgagcgt gtcgtccagg acgttcatgg aacgccccgg cgagtccatc gtcgccacgg
      301 cgatgccgtc ggcgccgacc tccacgcgaa gtgcgctgcg ggtgcccgta tccgtatccg
      361 tgtcggccat ggcgtcagac cctctcgatg atcgtggcga tgcccatgcc gccgcccacg
      421 cacagcgtcg cgcagccgag ggcgaggtcg cggcgctcca gctcgtcgag cagggtgccg
      481 aggatgatgc agccggtcgc gcccagcgga tgcccgagcg cgatcgcgcc gccgttgacg
      541 ttgacgcggt cgagcccgat ggccaggtcg cgcgcggtct tcatcggcac cgcggcgaag
      601 gcctcgttga tctcccacag gtcgatgtcg gctgccgcca ggctggcctt cgcgagcgcc
      661 ttgcggcagg ccggcgtcgg gccggtgagc atgatggtcg gctccgagcc ggtcacggcg
      721 acgctgcgga tgcgggcccg cggcgcgagg ccggcgcgct cgcccgcggc gcgcgagccg
      781 atcagcatca gcgccgcgcc gtcgacgatg cccgaggcgt tgccggcatg gtgcacgtgc
      841 gcgatccgct cgagcgtcgt gtacttgcgc agcgcggtgg cgtcgaagcc catcgcgccc
      901 atcgcggcga acgacggctc gagcctggcc atcgcctcga gcgaggcgtc ggggcggatc
      961 gtctcgtcct ccaccagcag cggcaggccc gcgatgtcgt gcaccggcag gatcgagcgc
     1021 ttgaagcgcc cggcctcgcg tgcggcggcc gcgcggtggt gcgagtcgcg tgcgtaggtg
     1081 tcgaggtcgg tgcgggagaa ggcttccagc gtcgcgatca ggtcggcgct gacaccctgg
     1141 gggatgaatc cgagcgcctg gttcacgcgc ggatccatga accacgcgcc gccgtcgctg
     1201 cccatcggcc agcggctcat cgactcgacc ccgccggcga cgaccaggtc ctcgaagccg
     1261 ctcgcgacct tggccgccgc gaggttcacc gcctccaggc cggaggcgca gaagcgcgac
     1321 agcgtgacac cggccgcgct ctcggcccag ccggcgtcca gcaccgcgat gcgcgcgatg
     1381 tcggcgcctt gctcgcccac cggcgtgacg cagccgagga cgacgtcgtc cacgagcgcc
     1441 gtgtcgagcg cgaggcggtc gcgcaggccc tgcagcaggc cgcgcagcag ccaggcgggc
     1501 gaggcctggt gcagccggcc gtccttcttg cccttgccgc gcggcgtgcg gacgtggtcg
     1561 tagatcagtg cgtcgttcat gagaggacct cccttgcggg ccgcggttcg agcgtcactt
     1621 cccgaggctc ttgcgcacgg tggcgttgat caggcgcagc tcggcgagcg acgcgtcgat
     1681 gtgctcgcgc ttgcgctcca ggtcggcgat cgccttgctg gtcttgtcga gcacgtactt
     1741 cagctgcacc gcgcggccct cgccgtgcgt gccgtagaga tcgaggtagt gcttgatctc
     1801 cgcgagcgag gagccgatgg ccttggcccg caggatcagc tgcagccgtg cgcggtcgcg
     1861 ccgcgtgtag acgcgcgtgc cgttgacgcg gcgcggcgcg agcaggccct tgtcctcgta
     1921 gaagcggatc gcgcgcggag agatgtcgaa ctcgcggcac agctccgtga tgccgaacag
     1981 ctggccggcg tcgtcgctgc gatggtcggc gaccacctcg tcggcggtct gggccttcgc
     2041 ccgggcggcg tgcagggaca tcgcgcggct cctcttgccc cgggcggcgt cagacgatgc
     2101 gctgcagctt cagcgcgacg ctcatgtcgc ccgacacctt gaacttcccg agcatgaagc
     2161 cggtcgtcgg ctgcagctcg cccgacagca ggtcgcccag cgtggcgagc ttcagcgaca
     2221 cggtgcagtc ggccggcagc gacgcgtcgt tggacaccgt attgggcacg tcgcgcgcgt
     2281 cgacgaggat cgcgccgtcg tcgccgcagt cgaacttcag gatggcgccg aggccgcagt
     2341 cgtcgccgac cttggcgcgc agctgggtgg tggcggtctg gagatccatg gtcgcgttcc
     2401 tcgtcgtcgt gtcggtgagg gaaggtcggg cgcggacgcg gcgtccgtgc ggggcgcgtc
     2461 gagcttaagc ggcgcgcctc gagcttcgca tcggtgtaat tcccgatcgg gaatcgagtt
     2521 gacgttaacg tcaccatgcc gcctaatgcg gcacgtcgca acgctcagcc tcgaaccgcc
     2581 catggcgatc gcgcctcgtg aaccgtcttc cgccttgccg ggcgccgggc cccgcccggg
     2641 ttaccgcagc gccttcgcgc cggggctgtt cgccgggcag cgcgtctggg tgacgggcgg
     2701 cggctcgggc atcgggcgcg cggtcgcgca cgagctcgcg tcgctgggcg cgcacgtgat
     2761 cctgagcggt cgcagtcccg acaagctcga acgcaccgcc gccgagatcg acgaggacgg
     2821 cgggcgctgc gggtggcgcg cgttcgacat ccgcgacgag gacgcggtca aggcgcaggt
     2881 cgaggccgag gtgcgcgagg gcggtgtcgt gcacggcctc gtcaacaacg ccggcggcca
     2941 gttcccggcg ccgatgcagg ccatcagcaa gcgcggcttc gaggccgtgg tcgccaacaa
     3001 cctgacgggc ggcttcctga tgatgcgcga gctgttcctg cacagcttcc aggcgcacgg
     3061 cggcgcgatc gtcaacatga ccgccgactt ccgcaacggc atgccgggca tggcgcactc
     3121 cggcgcggcg cgcgcgggca tgagcaacct cacgatgacg gcggccttcg agtgggcttc
     3181 gtcgggggtg cgcgtgaacg cggtcgcgcc gggctggatc gcctccagcg gcatggacac
     3241 ctacggcggc gccatgcgcg cgttgatccc gcagctcaag cgccacgtgc cgctgcggcg
     3301 catgggcctg gaggccgagg tgagcgcggc catcgtcttc ctgctgtcgc ccgccgccgc
     3361 cttcgtcacg ggcgtgacgc tgcagatcga cggcggcgcg tcgctgggtt cgccgctgtt
     3421 cccctccgtc gaccacgagc gctcggtgcc cttcgacggc ttccatcgcg cggcgacgcc
     3481 cgaggtgctg cgcgaagccg gcggcgatac ggatacgggc aggggttcgt gatgccggcg
     3541 ctcggctccg cgctcgaccc gcggtcgccc gccttcgcgg ccaacgcggc gcgcatggcc
     3601 gagcggctcg aggaggtgcg cgcgctcgag cgccaggtgg tcgccgcgag cgcttcgaag
     3661 cgggcgaagt tcgaggcgcg cggccagctg ctgccgcgcg agcgcgtggc gcagctgctc
     3721 gatcgcggca gcggcttcct ggagctgtcg gcgctcgcag gcctgggcat gcacgacgac
     3781 gacggccgca ggtcggtcca gggcggcggc tccatcgtcg gcatcgggct cgtggccggc
     3841 aagcgggtgc tggtctccgc gagcgacagc gcgctcaagg gcggcacggt ctcgccgatg
     3901 ggcctgcgca aggcgctgcg ggcgcagcag gtggcgctcg agaaccggct gcccatcgtc
     3961 tacctcgtcg aatcgggcgg cgccaacctg atgtaccaga gcgagatgtt cgtcgagggc
     4021 ggccgcagct tcgccaacca ggcacggctg tcggcggccg gcatcccgca ggtggccgtg
     4081 gtgcacgggt cgtcgaccgc gggcggcgcc tacctgcccg ggctgtcgga ctacgtcgtg
     4141 ctggtgcgcg ggcgatcgag catctacctg gccggcccgc cgctcgtgaa ggcggccatc
     4201 ggcgaggacg cgaccgacga ggagctcggc ggcgcgcaga cgcacgccga ggtcaccggc
     4261 ctgggcgaat acctgggcga ggacgacgcc cacgcgatcg cgatcgcgcg cgagctcgtc
     4321 gacaagctgc agtgggacgc cgcgccggcg ccgctcgcga gctcgcctcc gctgttcgat
     4381 gccgaggagc tgctgggcgt cgtgcctgcc gacgagcgcg agccctacga cgcgcgcgag
     4441 atcgtcgccc gcatcgtcga cgcctccgac ttcctggagt tcaagcccgg cttcggcgcc
     4501 gataccctct gcgggcatgc gcgcatcgag gggcggctgg tcggcctgct cgccaacaac
     4561 gggccgatcc agccgcaggg ctcgaacaag gcggcgcagt tcatccagct ctgcgatcag
     4621 accggcacgc cgctcgtctt cctgcagaac acgaccggct acatggtggg gtcggccgcc
     4681 gagcgcggcg gcgcgatcaa gcacggcgcg aagatgatcc aggccgtcgc caatgcgcgc
     4741 gtcgcgaagt tcaccgtcgt ggtcggcggc tccttcggcg ccggaaacta cggcatgtgc
     4801 gggcgcggct tcgatccgcg cttcatcttc agctggccct cggcgcgcac cgcggtgatg
     4861 ggcggggcgc aggccgccaa ggtgatggag atcgtgggcg cgggcaagct cgcccgcgcc
     4921 gggcagcagg ccgacgaggc ggcgctcagg gcgatgggcg agcagctgcg ccagcgcatc
     4981 gaccgcgagt cgggcgtgct gttcggcacc gcgcgcctgt gggacgacgg cgtcatcgac
     5041 ccgcgcgaca cgcgccgcgt gctgggcctc gcgctcgcgc tcgcgcacga ggccgacacg
     5101 cgcgtcctgc gtcccaacgc cttcggcgtc gcccgcttct gacatcgtcc cgacgaagga
     5161 ttcaccatgc acttcacgcc cgagcaccgc gcgctcgccg atacggtcac ccgtttcgtc
     5221 gagaaggagc tgaaccccta cgtgccgcag tgggaggcgg acgagatcta cccggcgcac
     5281 gaggtcttcg ccaagctcgg caagctcggg ctgctggggc tgaaataccc cgaggagtac
     5341 ggcggcgcgg gcctggactt cagctactcg gtcacgatgg ccgaggcgct gggcgagtgc
     5401 gcctgcgggg gcgtgccgat gtccatcggc gtgcagaccg acatgtgcac ccccgcgctc
     5461 gcgcgcttcg gctcggacga actgcgtcgc gagttcctcg cgccgtccat cgccggcgag
     5521 ctcgtgggtt gccttggcgt gagcgagccg ggcggcgggt cggacgtcgc cgcgatcagg
     5581 acgaccgcgc gcaaggacgg cggcgactac gtgatcaacg gcaccaagat gtggatcacc
     5641 aacggcctgc aggccgactg gtgctgcctg ctcgccaaca cgggcgaggg ccccgtccac
     5701 aagaacaaga gcctgatcgt cgtgccgatg gacgccccgg gcatcacgaa gcagaagatc
     5761 cgcaagatcg ggatggattc gtccgatacc gcgcagctgt tcttcgacga ggtgcgcgtg
     5821 ccgcagcgcc acctgatcgg ccaggagggc atgggattcg cgttccagat gatgcagttc
     5881 caggaggagc ggctgtgggg cgcggcgtcg tcgctgcgca gcctggatcg gctgatcgac
     5941 ctcacgatcg agtacacgcg cgatcgccac gccttcggca agagcgtgct ggacaaccag
     6001 gtggtgcact tccgcctcgc cgagctgcgc accgaggtcg cggccctgcg cgcgctgacc
     6061 taccaggcgt gcgagctcta cgtctcgggc aaggacgtca ccaagctcgc gtcgatggcc
     6121 aagctgaagt gcgggcgcct cagccgcgag gtcgccgaca gctgcctgca gttctggggc
     6181 ggcatgggct tcacgcgcga caacccgatc tcgcaggcct ggcgcgattc gcgcctgatc
     6241 tcgatcggcg gcggggcgga cgaggtgatg ctgggcatca tctgcaagct cgaaggcacg
     6301 cttcccaagg gctcgcactg agatggccga ggcgcgttcg gtgccgccgg cctgcgagac
     6361 gctcgccgtc tcctcgcgcg accgcgtctg gcacgtcatg ctgaaccggc cggccttgcg
     6421 caacgccatg tcgctgcgca tggtcgagga actccaggcc gtgctcgcgc aggcggagcg
     6481 ctcgggcgag gtgcgcgcga tcgtgctgcg cggcgcgggc gggcacttct gcgcgggcgc
     6541 ggacctgaag gacatgggcg cggcgcggca gcgcgagctc gcgggcgagc gcgacgcggt
     6601 ggcagaggcc aatgcgcgat tcggcgcgct gtgcgcggcc tt
//
LOCUS       DASVLN010000293         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_499985, whole genome shotgun sequence.
ACCESSION   DASVLN010000293 DASVLN010000000
VERSION     DASVLN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499985"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1005)
                     /locus_tag="VF453_07180"
     CDS             complement(10..1005)
                     /locus_tag="VF453_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7687470.1"
                     /translation="MTTDRRFALTPEALTMMDTIARTGSFAAAARELGKVPSALTYSV
                     RQLEDALDVLLFDRSSRQARLTAAGTELLNEGRRLLAEMDTVANRVHRVSTGWETQLT
                     IAADGVVSRHTLLELCEAFYALRPPELPAGTGPGTRLRLRTEVLAGTVEALVMGQADL
                     AIGLPVDIAVPAGIELRPLGEAPFVYVVGARHPLAAVEEPLTDAVLREQRGIAVADSA
                     QRMSPVTVNLQPGQDVLTVSSMQAKIEALMMNLGGGFVPEVLAREHVEAGRLVIKQVR
                     RQRLTAALSYGWRSAAAPQPHKPPQGLALQWWLQQLESPTTRSALLMRGGLCPGT"
     gene            complement(1002..2084)
                     /locus_tag="VF453_07185"
     CDS             complement(1002..2084)
                     /locus_tag="VF453_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7687471.1"
                     /translation="MQLLVRALGILLMLTALAVTLTDTPDRSVESLVARWAPPPSDFM
                     EIRGQLVHYRDEGPRDDPEPLVLLHGTASSLHTWQGWVGELRSRKRVITFDLPGFGLT
                     GPFAGEYAQDDYRGDTLARFTLDVLDALHVTRFDIGGNSLGGEVAWRVAALAPARVGR
                     LVLVDASGYAFRPEHVPIGFQLARLPVLGRIGEWLTPRVVVEQSVRDVYGDPSRVTQV
                     LVDRYFELLTREGNRRALGIRLREIEHDLAPQRVATLHVPTLILWGAQDRLIPPDNGE
                     HFHRDIAGSTLVVFPGLGHVPQEEDSHASVAPVKTFLGLDGPAAAPAPAASATTPATA
                     VSAPASRASGPYARAPAPPAQAREPA"
     gene            complement(2093..2863)
                     /locus_tag="VF453_07190"
     CDS             complement(2093..2863)
                     /locus_tag="VF453_07190"
                     /inference="COORDINATES: protein motif:HMM:NF003703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX7687472.1"
                     /translation="MRFLNISTYRFVEIDDPHALRDRLEASAVAAGLKGTILVAREGL
                     NLFVAGDEPALRGWVAASLHADPRFAGLATKDSFSDAPPFRRLKVKVKDEIIRMNHPA
                     IRPQAGRAPALAPAVLARWLAQGRDDDGRPLALVDTRNGFEVDAGTFEGAHDWRIHKF
                     SDFPAAFDAHRAELEGKTIVSFCTGGIRCEKAALYMAGHGRPDALQLEGGILGYFAAT
                     GGAAPGWTGDCFVFDERRTLDAGLAPGDPPPCEAATQD"
BASE COUNT          394 a         1117 c         1202 g          399 t
ORIGIN      
        1 cggcgtccgt catgtgcccg ggcacagccc gccgcgcatc agcagcgcgc tgcgcgtggt
       61 cgggctctcg agctgctgca gccaccattg cagcgcgagg ccctgcggcg gcttgtgcgg
      121 ctgcggcgcg gcggcgctgc gccagccgta ggacagcgcg gccgtcaggc gctggcgccg
      181 cacctgcttg atgacgagcc ggccggcctc gacatgctcg cgcgccagca cctccggcac
      241 gaagccgccg cccaggttca tcatcagcgc ctcgatcttg gcctgcatgc tgctgacggt
      301 gagcacgtcc tggcccggct ggaggttcac ggtgacgggc gacatgcgct gtgccgagtc
      361 ggcgaccgcg atgccgcgct gctcgcgcag caccgcgtcg gtcagcggct cctcgaccgc
      421 cgcgagcgga tggcgcgcgc cgaccacgta gacgaagggc gcctcgccga gaggccgcag
      481 ctcgatgccc gcgggcaccg cgatgtccac cggcagcccg atcgcgaggt cggcctggcc
      541 catcaccagc gcctcgacgg tgcccgcgag cacctcggtg cgcagccgca ggcgcgtgcc
      601 cgggccggtg ccggcgggca actccggcgg gcgcagcgcg tagaaggcct cgcacagctc
      661 gagcagcgtg tggcgcgaga cgacgccgtc ggccgcgatg gtcagctgcg tctcccagcc
      721 ggtggacacg cggtgcacgc gattggcgac ggtgtccatc tcggcgagca gccgccggcc
      781 ctcgttgagc agctcggtgc ccgcggccgt caggcgcgct tggcgcgagc tgcggtcgaa
      841 cagcagcacg tccagcgcgt cctcgagctg gcgcacgctg taggtcaatg ccgagggcac
      901 cttgccgagc tcgcgcgccg cggccgcgaa gctgccggtg cgggcgatgg tgtccatcat
      961 cgtgagggcc tcgggcgtca gcgcgaagcg gcggtcggtg gtcatgcggg ttccctggcc
     1021 tgggcaggcg gagcgggcgc gcgggcgtac ggcccggacg cgcgggaagc gggcgcggag
     1081 acagccgtgg ccggtgtcgt cgcggaagcg gccggcgccg gcgcggcggc cgggccgtcg
     1141 aggccgagga aggtcttcac cggcgcgacg ctcgcgtgcg agtcctcttc ctgcggcacg
     1201 tggccgagcc cggggaagac gaccagcgtg ctgcccgcga tgtcgcggtg gaagtgctcg
     1261 ccgttgtcgg gcgggatgag gcgatcctgc gcgccccaca ggatcagcgt cggcacgtgc
     1321 agcgtcgcga cgcgctgcgg cgcgaggtcg tgctcgatct cgcgcagccg gatgcccagc
     1381 gcgcgccggt tgccctcgcg cgtgagcagc tcgaagtagc ggtcgaccag cacctgcgtg
     1441 acgcgcgacg ggtcgccgta gacgtcgcgc acgctctgct cgacgaccac gcgcggcgtc
     1501 agccattcgc cgatgcgccc cagcaccggc aggcgcgcga gctggaagcc gatcggcacg
     1561 tgctcggggc ggaacgcgta gcccgacgcg tcgaccagca ccagccgccc gacgcgcgcc
     1621 ggcgcgagcg ccgcgacgcg ccatgccact tcgccgccga gcgaattgcc gccgatgtcg
     1681 aagcgggtga cgtgcagcgc gtccagcacg tcgagcgtga agcgggcgag cgtgtcgccg
     1741 cggtagtcgt cctgcgcgta ctcgcccgcg aacgggccgg tgaggccgaa gcccggcaag
     1801 tcgaaggtga tgacgcgctt cctcgaccgc agctcgccga cccagccctg ccatgtgtgc
     1861 aggctggacg cggtgccatg cagcagcacc agcggctcgg ggtcgtcgcg cgggccctcg
     1921 tcgcggtagt gcaccagctg gccgcggatc tccatgaagt ccgacggcgg cggcgcccag
     1981 cgcgcgacca ggctctcgac gctgcggtcc ggcgtgtcgg tcagcgtgac cgcgagcgcg
     2041 gtgagcatca gcaggatgcc gagggcgcgg acgaggagct gcatggcgcg cgtcagtcct
     2101 gcgtcgccgc ctcgcacggc ggcgggtcgc ccggcgcgag cccggcgtcg agcgtgcggc
     2161 gctcgtcgaa gacgaagcag tcgcccgtcc agcccggtgc cgcgccgccg gtcgccgcga
     2221 agtagccgag gatgccgccc tcgagctgca gcgcgtcggg ccggccgtgc ccggccatgt
     2281 atagcgccgc cttctcgcag cggatgccgc cggtgcagaa gctcacgatc gtcttgccct
     2341 cgagctcggc gcggtgggcg tcgaaggcgg cggggaagtc gctgaacttg tggatgcgcc
     2401 agtcgtgcgc gccctcgaag gtcccggcgt cgacctcgaa gccgttgcgc gtgtcgacga
     2461 gcgcgagcgg gcggccgtcg tcgtcgcggc cctgcgcgag ccagcgcgcg agcaccgccg
     2521 gcgcgagcgc gggcgcgcgg cccgcctgcg gccggatcgc gggatggttc atgcggatga
     2581 tctcgtcctt gaccttcacc ttcaggcgcc tgaacggcgg cgcgtcgctg aagctgtcct
     2641 tggtcgcgag gccggcgaag cgcggatcgg cgtgcagcga cgcggcgacc cagccgcgca
     2701 gcgcgggctc gtcgccggcg acgaacaggt tcagcccctc gcgggcgacg aggatggtgc
     2761 ccttgaggcc ggccgcgacc gcgctcgcct cgaggcggtc gcgcagcgcg tgcgggtcgt
     2821 cgatctcgac gaagcggtag gtggagatgt tcaggaagcg catggagccg gggagggcgc
     2881 ggacggccgc gcgaacgcgt attctggggc ctcgccggcg gatcggctgg cgcggggccg
     2941 gcggtgtacc ctccggggct cgccctctgc tccgaggaac gccttgacgc caccgccatc
     3001 gatcccgccc tatcccatcg gtacgcccgg cacgccctgg ggcgacgccg agcgcgccgc
     3061 ctggctcgcg agccagcgcg tgcggcgcag ctatgccggc gaggtcgtcg cg
//
LOCUS       DASVLN010000294         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_229515, whole genome shotgun sequence.
ACCESSION   DASVLN010000294 DASVLN010000000
VERSION     DASVLN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229515"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="VF453_07195"
     CDS             <1..1816
                     /locus_tag="VF453_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7687473.1"
                     /translation="PAVAAGDPPRAAAPDSLVDQADHLHAFARTGLVGNAVGAIVVAA
                     LHARLVPAGELLAWAALFSLAMAARAVLTATYAGRWRPDPAALPRWRARYLAGVMSTG
                     ALWGVAVWLFYPHGGELEHAALLLTVYSFCISAVPALATQLDAFVAFVALTFVPMIAR
                     IAFAPGAGGGWTLAAILGLIFGITVMLGRSFQRSFARVLALKLRGQQLLAQLHSEKAA
                     ADQARREAETANRAKTQFFAAASHDLRQPLHALGLFAEALRHRVGADAEVAHLVHSIN
                     GSVDALEGLFSELLDITKLDTGAVEARPEHFGCEAMFARLRLHFEPVAFEKGLALRFH
                     GGRHFAFADPLLVERVVRNLLSNAIRYTNDGGVVVAARKRGDRLRLEVWDTGLGIRPN
                     EQERIFEEFYQVAGDAPALDPTQRKGLGLGLAIVRRLARVLEAPLRLRSEPGRGTVFS
                     LEVALGKRPRPSEPDVPVRTTLGLALERRLVAVVEDDPAVLGGLDVLLKSWGADVAAF
                     PSHAACMAWLAAAGERPAARPDLLIADYRLEDGRDGFQVLEAMRATFGRALPAVIVTG
                     SLMSDQEAQANAHDVHLLLKPVVPAKLRAMIAFKLGQR"
BASE COUNT          222 a          754 c          744 g          277 t
ORIGIN      
        1 gcccgccgtg gccgccggcg acccgccgcg cgccgccgcg cccgattcgc tcgtcgacca
       61 ggccgaccac ctgcacgcct tcgcgcgcac cggcctcgtg ggcaacgccg tcggcgcgat
      121 cgtcgtcgcg gcgctgcacg cgcgcctcgt gccggcgggc gagctgctcg cctgggccgc
      181 gctcttctcg ctcgcgatgg ccgcgcgcgc cgtgctcacc gccacctatg ccggccgctg
      241 gcgtcccgac ccggcggcgc tgccgcgctg gcgcgcgcgc tatctcgcgg gcgtgatgtc
      301 caccggcgcg ctgtggggcg tggcggtctg gctgttctat ccgcacggcg gcgagctgga
      361 gcacgccgcg ctgctgctga cggtctacag cttctgcatc tccgccgtgc cggcgctggc
      421 gacgcagctg gacgcgttcg tcgccttcgt cgcgctgacc ttcgtgccga tgatcgcccg
      481 catcgccttc gcgcccgggg ccggcggcgg ctggacgctc gcggccatcc tcgggctgat
      541 cttcggcatc acggtgatgc tggggcgcag cttccagcgc tcgttcgcgc gcgtgctggc
      601 gctgaagctg cgcgggcagc agctgctcgc ccagctgcac agcgagaagg ccgccgccga
      661 ccaggcgcgg cgcgaggccg agacggccaa ccgcgcgaag acccagttct tcgccgccgc
      721 gagccacgac cttcgccagc cgctgcacgc gctgggcctg ttcgccgagg cgttgcggca
      781 ccgcgtcggc gcggacgccg aggtcgcgca cctcgtgcac agcatcaacg gctcggtcga
      841 cgcgctggag gggctgttct ccgagctgct ggacatcacc aagctcgaca ccggcgccgt
      901 cgaggcgcgg ccggagcact tcggctgcga ggcgatgttc gcgcgcctgc ggctgcactt
      961 cgagccggtg gcgttcgaga aaggcctcgc gctgcgcttc cacggcggcc gccacttcgc
     1021 gttcgctgat ccgctgctgg tcgagcgcgt cgtgcgcaac ctgctgtcca acgcgatccg
     1081 ctacaccaac gacggcggcg tcgtcgtcgc ggcccgcaag cgcggcgacc ggctgcggct
     1141 ggaggtctgg gacaccggcc tgggcatccg cccgaacgag caggaacgca tcttcgagga
     1201 gttctaccag gtcgccggag acgcgcccgc gctcgacccg acgcagagga agggcctggg
     1261 cctcgggctg gcgatcgtgc gccggctcgc gcgcgtgctc gaggcgccgc tgcggctgcg
     1321 ctcggagccg gggcgcggca cggtgttctc gctggaggtg gcgctcggca agcggccgcg
     1381 gccgtccgag cccgacgtcc ccgtgcgcac gacgctgggc ctcgcgctgg agcggcggct
     1441 ggtggcggtg gtcgaggacg acccggccgt gctgggtggc ctggacgtgc tgctcaagag
     1501 ctggggcgcg gacgtcgccg ccttcccgag ccacgctgcc tgcatggcct ggctggccgc
     1561 cgccggcgag cgccccgccg cccgacccga cctgctgatc gccgactacc ggctcgagga
     1621 cggccgcgac ggcttccagg tgctcgaggc gatgcgcgcc accttcggcc gggcgctgcc
     1681 cgcggtcatc gtcaccggca gcctgatgtc cgaccaggag gcccaggcca acgcgcacga
     1741 cgtgcacctg ctgctcaagc cggtggtgcc ggccaagctg cgagcgatga tcgcgttcaa
     1801 gctggggcag cgctgatgcg ggaacgaggc gcctgcctgc gcatgaccac ccggcccgcg
     1861 catgcgtcgc gggcccgccg ggagggaggc gccttcagcg cggtgcgggc gcgcggaggc
     1921 actcgccctc cggcgcgcca gggggcggcc gtccgatgag cgcgggcatc gatccgatcc
     1981 agcgagcggt gcgcgtc
//
LOCUS       DASVLN010000295         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_122827, whole genome shotgun sequence.
ACCESSION   DASVLN010000295 DASVLN010000000
VERSION     DASVLN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122827"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF453_07200"
     CDS             complement(<1..190)
                     /locus_tag="VF453_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687474.1"
                     /translation="MVVDARGVGVVDAVRAGGGSNAHAGRDGGRVVDGALGHAAGAPN
                     VGLSRTSRRAALPAKRVAA"
     gene            202..1362
                     /locus_tag="VF453_07205"
     CDS             202..1362
                     /locus_tag="VF453_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX7687475.1"
                     /translation="MIPLPNARPSFVLALAASALLVACGGDGGGSTAGAGGTTCPPGD
                     TYGSGTCSGGTGTGGYHATALVSDVAGAAAHQDAHLVNAWGLAFNPTGFSWVSNAGTS
                     SSTLYDGNGVPQPLVVTIDAGANGGSHPSGIVFNGTSDFAIASGGATGGAPFIFATLE
                     GQIDAWAPSVDGTHALVEVDNGAAGAGYTGLAIGTSGASNLIYAANFAAGTVDVFDAR
                     YAPVTTTGGFVDPTLPAGYAPFGIQQIAGKIYVAYAKKDPATGDEMHAAGLGLVDVFD
                     AQGTLLTHLVAAGGALDAPWGLAMAPSGFGALSGMLLVGNFGDGTIHAYDPATGAAVG
                     ALDDASGAPLTVDGLWALQFGNGVNAQPTTTLFYTAGPSDETHGRYGRIDAN"
     gene            1551..1847
                     /gene="rpsT"
                     /locus_tag="VF453_07210"
     CDS             1551..1847
                     /gene="rpsT"
                     /locus_tag="VF453_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX7687476.1"
                     /translation="MATSSKAKKRTVRLASGRKRARQDVKLNAHNSSLRSKFRTVIKN
                     VQKAVAAGDGAKAAELFKSAQSTIDSIADKGLFHKNKAARHKSRLAAKVKALAA"
     gene            complement(1940..2293)
                     /locus_tag="VF453_07215"
     CDS             complement(1940..2293)
                     /locus_tag="VF453_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEX7687477.1"
                     /translation="MNPPAIPRQFFIQGVTLDGRTFRPSDWAERLAGAMSSFRPGGVR
                     AGIGSFIGYSPYCVPRLCGGVKCVIVDERLKGLEPMAWDFVMNFARDNKLRVVEGVLL
                     PDGSIQPVQPTPPAG"
BASE COUNT          375 a          922 c          853 g          335 t
ORIGIN      
        1 gtgccgcgac gcgcttcgcc ggcaatgctg cgcgtcggct tgtccggctc agtccgacgt
       61 taggcgcacc ggccgcatgg ccgagcgcgc cgtcgacgac cctgccgcca tcgcggccgg
      121 cgtgcgcgtt tgaaccgccg cccgcgcgaa cggcatccac gacgccgacg ccgcgggcgt
      181 ccacaaccac ggagcgagtc catgatcccc cttccgaacg cacgtccctc cttcgtcctc
      241 gcgctcgcgg cgagcgcgct gctcgtcgcc tgcggcggcg acggcggcgg cagcaccgcc
      301 ggcgccggcg gcacgacctg cccgccaggc gacacctacg gctcggggac ctgttcgggc
      361 ggcaccggca ccggcggcta ccacgcgacg gcgctggtct ccgacgtcgc cggcgccgcg
      421 gcgcaccagg atgcgcacct cgtcaacgcc tggggcctgg cgttcaaccc gacgggcttc
      481 tcgtgggtct cgaacgcggg cacctcgagc tcgacactgt acgacggcaa cggcgtcccg
      541 cagccgctgg tcgtcaccat cgatgcgggc gccaacggcg ggagccaccc gtcgggcatc
      601 gtgttcaacg gcaccagcga cttcgcgatc gcttcgggcg gcgcgaccgg tggcgcgccc
      661 ttcatcttcg cgacgctgga gggccagatc gacgcctggg cgccgtcggt cgacggcacg
      721 cacgcgctgg tcgaggtcga caacggcgcc gccggcgcgg gctacacggg cctggcgatc
      781 ggcaccagcg gcgcgagcaa cctgatctac gcggccaact tcgcggccgg cacggtcgac
      841 gtgttcgatg cccgctacgc gcccgtcacc acgaccggcg gcttcgtcga cccgacgctg
      901 ccggccggct acgcgccctt cggcatccag cagatcgcgg gcaagatcta cgtggcgtat
      961 gccaagaagg atcccgcgac cggcgacgag atgcacgccg cgggcctggg cctggtcgac
     1021 gtcttcgacg cgcagggcac gctgctgacc cacctcgttg cggccggcgg cgcgctggat
     1081 gcgccgtggg gcctggcgat ggctccgagc ggcttcggcg cgctgtcggg catgctgctg
     1141 gtcggcaatt tcggcgacgg cacgatccac gcctacgacc ccgccacggg cgccgcggtg
     1201 ggggcgctcg acgacgccag cggcgcgccg ctgaccgtcg acggcctgtg ggcgctgcag
     1261 ttcggcaacg gcgtcaacgc ccagccgacg acgacgctgt tctacacggc cgggccgagc
     1321 gacgagacac acggccgcta cggccgcatc gacgccaact gagcgcccgc gcgggctcct
     1381 ggccgccagc tcgaccgtcg cgatggccac ccgccggcgg agcgctcgcg acgcgcccgc
     1441 gacacgcttt tcgggctcac tcacgcatcg gccgtattgc ggacgcgcca tttcggccga
     1501 agcgtggtat gatcgaagac tttgcacctc acccggagca tcaataccaa atggcaacct
     1561 cttccaaagc caagaagcgc accgtccgcc tggcctcggg ccgcaagcgc gcccgccagg
     1621 acgtgaagct gaacgcgcac aactcgtcgc tgcgctcgaa gttccgcacc gtcatcaaga
     1681 acgtccagaa ggctgtcgcc gccggtgacg gcgcgaaggc cgccgagctg ttcaagtccg
     1741 cccagtcgac gatcgactcg atcgccgaca agggtctctt ccacaagaac aaggccgcgc
     1801 gccacaagag ccgcctggcc gccaaggtga aggcgctggc cgcctgaagc caattccggg
     1861 tgagttagtc gagaagggct cgcgtcgcga gcccttttcc attggggcac cgcatcggga
     1921 gatgccggcg ccccggggct cagccggccg gcggcgtcgg ctggacgggc tggatgctgc
     1981 cgtcgggcag cagcacgccc tcgacgacgc gcagcttgtt gtcgcgcgcg aagttcatga
     2041 cgaagtccca ggccatgggc tccaggccct tgagccgctc gtcgacgatc acgcacttga
     2101 ccccgccgca cagccgcggc acgcagtacg gcgaatagcc gatgaacgag ccgatgcccg
     2161 cgcgcacgcc gcccgggcgg aagctggaca tggcgcccgc gagccgctcc gcccagtcgc
     2221 tcgggcggaa ggttcggccg tccagggtga caccctggat gaagaattgg cgggggatgg
     2281 ccggcggatt cattgaaggc gagtcgacgg ggaacctcgt attgtgcctt ggggtgttgc
     2341 gtcgcagcaa acatcagggt tacacgctcg atgccgaatc ctcgtcgggg acgcgccctt
     2401 agaatgtccg tcccccggcc gaggcgacgg cgcggccgtc ccgcatgccg ccgccgcgcg
     2461 aggccttccc gccccgatgc cgcgc
//
LOCUS       DASVLN010000296         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_284548, whole genome shotgun sequence.
ACCESSION   DASVLN010000296 DASVLN010000000
VERSION     DASVLN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284548"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VF453_07220"
     CDS             complement(<1..837)
                     /locus_tag="VF453_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7687478.1"
                     /translation="MAELQLQGLRRTYANGVTAVDGIDLTVRDGEFMVLVGPSGCGKS
                     TTLRMIAGLEDISAGHLRIGGEDMTHVAPADRGVAMVFQSYALYPHMTVAENMGFALR
                     IAGRPKAEIEASVRKAADALQLAHLLDRKPRALSGGQRQRVAIGRAIVRSPKVFLFDE
                     PLSNLDASLRTQMRLEMARLHEQLGVSVVYVTHDQVEAMTLGDRIAVFNSGRIEQVGT
                     PSEVYRQPVNQFVAGFLGAPRMNFLPARAFRGTAGPGLEIAGAGRLALPEGCDAALAA
                     VQV"
     gene            complement(864..2225)
                     /locus_tag="VF453_07225"
     CDS             complement(864..2225)
                     /locus_tag="VF453_07225"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX7687479.1"
                     /translation="MNDLTNKSLPAPLLPAAFQLGVSTSAAQIEGHAPGDGAGPSIWD
                     AFCAQPGKIKDGSNILVACDHIHRWAEDIERMRWLGLDAYRFSIAWPRVQPDGRGAWN
                     EAGFGFYDRLVDGLLEAGIAPHATLHHWDLPQALQEAVGGWQSRETCARFADYAVEVL
                     RRLGDRLVTIATLNEPWCVATLGYEKGQFAPGLKDRAAAMQVSHHLLLGHGLALKAMR
                     EAARGMAKSPQLGIVLNHTPAFAAEKTPADLQAARIADGLDVRWYMDPLFRGAYPADA
                     VESLGADAPKVEPGDFDVIRQPMDFLGINMYTRNWVSTRVPPIPSPNLRGVNDMGWEI
                     VPEVLTQHLVRLTRDYMPPPMIITENGMANADVVTAAGVEDVPRVDYVREHLAAVARA
                     IELGADVRGYMYWSLLDNFEWDSGYAKRFGLFRVDYETQARTPKRSAHWYRDFIAAHK
                     ARR"
     gene            complement(2247..3083)
                     /locus_tag="VF453_07230"
     CDS             complement(2247..3083)
                     /locus_tag="VF453_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7687480.1"
                     /translation="MSHAERFHPARVAAFVLVAAGVVVLAAPFWFTFVFATHARTEIF
                     ASPPPVWFGGHLLNNIRLLLERLPFWHNLWQSLYVATMTTALNLLLCSLAGYALAVHE
                     FAGKRALFALVVGSMMLPSFLNMIPTFMEMDFLGWIDEPRALYVPGAASALGIFLMRQ
                     YIGSAIPRDLVDAARMDGCGELRTFWSVVLPLCGPALGTLGLITFINSWNNFVGPLVV
                     MHDREHYTLPLALRSMQSPNNTEWGALMAGSAIAMLPLLVAFVFSARRLVAGLTAGAV
                     RG"
     gene            complement(3070..>3942)
                     /locus_tag="VF453_07235"
     CDS             complement(3070..>3942)
                     /locus_tag="VF453_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7687481.1"
                     /translation="RKLAPYALISPFFLLFALFGLFPLLFSIWLSLHQWDAAEGLAAM
                     HWVGLDNYRFALTDPWFRKSLLNTLWFAVASGVPQHLVALPLAFFIHTTLKRTRNVVV
                     GAYFVPYVTSSVAIALIFTTLFSKDFGVVNALIGRPIDWLGDAFWAHVTIAFVVFWRY
                     LGWNVVLYLSALQVISSDLYEAATLDGASRWQQFRLIALPLLKPMMFFAVTLTLIGNL
                     QLFEEPFILIPPDAGIVPSVMTTAMFMYRMAFADGDFGTASAVSWLLFFLIAALAYGN
                     GRLFGKEAQSDVAR"
BASE COUNT          638 a         1343 c         1425 g          536 t
ORIGIN      
        1 cacctgcacc gcggcgagcg ccgcgtcgca gccctcgggc agcgcgagcc ggccggcgcc
       61 ggcgatctcc aggccgggcc ccgccgtgcc gcggaacgcc cgcgccggaa ggaagttcat
      121 gcgcggcgcg ccgaggaagc cggccacgaa ctggttgacc ggctggcggt agacctccga
      181 cggcgtgccg acctgctcga tgcggccgct gttgaacacc gcgatgcggt cgcccagcgt
      241 catcgcctcg acctggtcgt gcgtgacgta gacgacggag acgccgagct gctcgtgcag
      301 ccgcgccatc tccagccgca tctgcgtgcg cagcgacgcg tcgaggttgg acagcggctc
      361 gtcgaacagg aacaccttcg ggctgcgcac gatcgcgcgc ccgatcgcca cgcgctggcg
      421 ctggccgccg gacagcgcgc gcggcttgcg atccagcagg tgcgcgagct gcagcgcgtc
      481 ggcggccttg cgcacggagg cctcgatctc cgccttcggc cggccggcga tgcgcagcgc
      541 gaagcccatg ttctccgcca ccgtcatgtg cgggtagagc gcgtagctct ggaacaccat
      601 ggcgacgccg cggtcggcgg gcgcgacgtg ggtcatgtcc tcgccgccga tgcgcaggtg
      661 gccggcgctg atgtcctcga ggcccgcgat catgcgcagc gtcgtcgact tgccgcagcc
      721 cgacggcccg acgagcacca tgaactcgcc gtcgcgcacg gtcaggtcga tgccgtcgac
      781 cgcggtgacg ccgttcgcat aggtgcggcg caggccctgg agctggagtt cggccatctc
      841 tgcttttccg actctgtgag aagttagcgg cgcgccttgt gcgcggcgat gaaatcgcgg
      901 taccagtgcg cgctgcgctt gggcgtgcgc gcctgggtct cgtagtcgac gcggaacagg
      961 ccgaagcgct tggcgtagcc ggagtcccac tcgaagttgt ccagcaggct ccagtacatg
     1021 tagccgcgca cgtcggcgcc gagctcgatc gcgcgcgcga ccgcggcgag gtgctcgcgc
     1081 acgtagtcca cgcgcggcac gtcctcgacg cccgcggccg tcacgacgtc cgcgttggcc
     1141 atgccgttct cggtgatgat catcggcggc ggcatgtagt cgcgcgtcag gcgcaccagg
     1201 tgctgcgtca gcacctcggg cacgatctcc cagcccatgt cgttgacgcc gcgcaggttg
     1261 ggcgacggga tcggcggcac gcgcgtgctg acccagttgc gcgtgtacat gttgatgccg
     1321 aggaagtcca tcggctggcg gatgacgtcg aagtcgcccg gctcgacctt cggcgcgtcg
     1381 gcgccgaggg actcgacggc gtcggccggg tacgcgccgc ggaacagcgg atccatgtac
     1441 cagcgcacgt ccaggccgtc ggcgatgcgg gcggcctgca ggtcggccgg cgtcttctcg
     1501 gccgcgaagg ccggcgtgtg gttcaggacg atgcccagct gcggcgactt cgccatgccc
     1561 cgcgccgcct cgcgcatcgc cttcagcgcg agtccgtggc ccagcagcag gtggtgcgag
     1621 acctgcatcg ccgccgcgcg gtccttcagg cccggcgcga actggccctt ctcgtagccc
     1681 agcgtcgcga cgcaccaggg ctcgttgagg gtcgcgatcg tgacgaggcg gtcgccgagg
     1741 cggcgcagca cctcgaccgc gtagtcggcg aagcgcgcgc aggtctcgcg cgactgccag
     1801 ccgcccacgg cctcctgcag cgcctgcggc aggtcccagt ggtgcagcgt cgcgtgcggc
     1861 gcgatgccgg cctccagcag gccgtcgacg aggcggtcgt agaagccgaa gccggcctcg
     1921 ttccacgcgc cgcggccgtc gggctgcacg cgcggccagg cgatcgagaa gcggtaggcg
     1981 tccaggccca gccagcgcat gcgctcgatg tcctcggccc agcggtggat gtggtcgcag
     2041 gcgacgagga tgttcgagcc gtccttgatc ttgccgggct gcgcgcagaa cgcgtcccag
     2101 atggacgggc ctgcgccgtc gccgggcgca tggccctcga tctgcgcggc gctcgtggac
     2161 acgccgagct ggaacgcggc gggcagcagc ggagcgggca aggacttgtt cgtgaggtcg
     2221 ttcatggagt cggtcgaagt gatggatcag ccgcggaccg cgccggcggt gaggccggcg
     2281 acgaggcgcc gcgcggagaa gacgaaagcc acgagcaggg gcagcatcgc gatggcgctg
     2341 ccggccatca gcgcgcccca ttcggtgttg ttggggctct gcatgctgcg cagcgcgagc
     2401 ggcagcgtgt agtgctcgcg gtcgtgcatg acgacgagcg gcccgacgaa gttgttccag
     2461 gagttgatga aggtgatcag gcccagcgtc ccgagcgccg gcccgcacag cggcagcacg
     2521 acgctccaga acgtgcgcag ctcgccgcag ccgtccatgc gcgccgcgtc gaccaggtcc
     2581 cgcgggatcg cgctgccgat gtactgccgc atcaggaaga tgcccagcgc gctcgcggcg
     2641 cccggcacgt acagcgcgcg cggctcgtcg atccagccga ggaagtccat ctccatgaac
     2701 gtcgggatca tgttcaggaa gctgggcagc atcatcgagc ccacgaccag cgcgaagagg
     2761 gcgcgcttgc ccgcgaactc gtgcaccgct aacgcgtagc ccgccagcga gcacagcagc
     2821 aggttcagcg ccgtcgtcat cgtcgcgacg tacaggctct gccagaggtt gtgccagaac
     2881 ggcaggcgct ccaggagcag ccggatgttg ttcagcaggt gccctccgaa ccacacgggc
     2941 ggcggcgacg cgaagatctc ggtgcgcgca tgcgtcgcga acacgaaggt gaaccagaac
     3001 ggcgcggcca gcacgacgac gcccgcggcg acgaggacga acgcggccac gcgcgcggga
     3061 tggaacctct cagcgtgcga catcgctctg cgcctccttg ccgaacaggc gcccgttgcc
     3121 gtaggccagc gccgcgatga ggaagaacag cagccacgag acggccgagg cggtgccgaa
     3181 gtcgccgtcc gcgaaggcca tgcggtacat gaacatcgcc gtcgtcatca ccgagggcac
     3241 gatgccggcg tccggcggga tgaggatgaa cggctcctcg aacagctgca ggttgccgat
     3301 cagcgtgagc gtcaccgcga agaacatcat cggcttgagc agcggcagcg cgatgaggcg
     3361 gaactgctgc caccggctcg cgccgtccag cgtcgcggcc tcgtacaggt cgctggagat
     3421 cacctgcagc gccgacaggt acagcacgac gttccagccc aggtagcgcc agaacacgac
     3481 gaaggcgatc gtcacgtgcg cccagaaggc gtcgccgagc cagtcgatcg gccgcccgat
     3541 cagcgcgttg acgaccccga agtccttcga gaacagcgtc gtgaagatca gcgcgatcgc
     3601 gacgctcgac gtcacgtagg gcacgaagta ggcgccgacg acgacgttgc gcgtgcgctt
     3661 gagcgtggtg tggatgaaga acgcgagcgg cagcgcgacc aggtgctgcg gcacgcccga
     3721 ggcgaccgcg aaccacagcg tgttcagcag cgacttgcgg aaccacgggt cggtgagcgc
     3781 gaagcggtag ttgtcgaggc cgacccagtg catcgcggcc aggccctcgg cggcgtccca
     3841 ctggtgcagc gacagccaga tcgagaacag cagcgggaac aggccgaaca gcgcgaacag
     3901 caggaagaac ggcgagatca gcgcgtacgg cgcgagcttg cg
//
LOCUS       DASVLN010000297         5194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_445892, whole genome shotgun sequence.
ACCESSION   DASVLN010000297 DASVLN010000000
VERSION     DASVLN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445892"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VF453_07240"
     CDS             <1..316
                     /locus_tag="VF453_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7687482.1"
                     /translation="AAEMLGRVGLGARLGHYPRVLSGGEQQRVALARAFVVRPQVLLA
                     DEPTGSLDFATGETVMQLMFDLNREVGTTLVLVTHDRAIAARCDRQLHIEAGRLVEPE
                     PA"
     gene            complement(540..737)
                     /locus_tag="VF453_07245"
     CDS             complement(540..737)
                     /locus_tag="VF453_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687483.1"
                     /translation="MQIDMPKRAFILVLNAMEHYAKHLAADEEDPGPSGDDAMYLRYV
                     HKELCDKFIGEDGKVKADFLP"
     gene            complement(747..1301)
                     /locus_tag="VF453_07250"
     CDS             complement(747..1301)
                     /locus_tag="VF453_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687484.1"
                     /translation="MGKLLNDTEVKERAAARGLTPGCRLWAAGAKIDGDCFRKGNGPK
                     NPAIYLRWSDIGVRGKSGKIEGDDLARATVDPGDGLSLFIEQTVQGNFTQLGSSPDGA
                     FGKAYLEKLAKEEGYTEVSQIHWFKLAERTIPVGLEVVYDGHPPGHCTLTVTRSMTVG
                     EFVSLVGEHLGFDYAGSDIYGPRG"
     gene            complement(1472..2293)
                     /locus_tag="VF453_07255"
     CDS             complement(1472..2293)
                     /locus_tag="VF453_07255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7687485.1"
                     /translation="MNSKTLAALIGTLVLPMAVMAQNIATVNGKPVPKSRVDALVNMA
                     THGQPDQAPPEMLTQARDQVVMREILSQAAEKAGVMNTPEYKSQLELTRQTVLINTLF
                     QNYAKSHPVSDAEAKAEYDKLKAAQGNEYHAHHILVDKEEDAKKLIAQIKAGSKFEDV
                     AKKESKDTQSATNGGDLDWAPATNYVPEFATALESLKPGQMTDTPVKTQYGYHIIRLD
                     ETRAAKFPSFDEAKERIKQKLTQDKLQEYQEQLRKSAKTDYKFAADEGAPAPAKK"
     gene            complement(2488..2769)
                     /locus_tag="VF453_07260"
     CDS             complement(2488..2769)
                     /locus_tag="VF453_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX7687486.1"
                     /translation="MATLTRPQLHAAMTRALQEALAPSALELVDDSAAHAGHAGAREG
                     AHYNLRIVSERFAGLARVARHRLVYDALRPWMADGVHALAIDARAPSEG"
     gene            complement(2780..3433)
                     /locus_tag="VF453_07265"
     CDS             complement(2780..3433)
                     /locus_tag="VF453_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEX7687487.1"
                     /translation="MKILLDFLPLAFFFLTYKVAGLHPDASAQFATHWLGALVQGGVV
                     GPKEAPVLLATVVVAAATLLQVVWMKANRRRIDLLLWINLTLILVLGGITVWLHNETF
                     IKWKPSVVYWLMGLALWSSQTLFGHNLLRQTLGAEIDLPDAVWRRMNFLFVAFFGLMG
                     LVNVWVVYSFSTDAWAKFHTFGTTGLMVVFVLGVGVYMSRHLDHAAPPAATDESRSS"
     gene            complement(3458..3904)
                     /gene="msrB"
                     /locus_tag="VF453_07270"
     CDS             complement(3458..3904)
                     /gene="msrB"
                     /locus_tag="VF453_07270"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7687488.1"
                     /translation="MTATPPRLTNPHPHDDATYPVRRSDEEWRAKLDPMQYQVARHAA
                     TERAFTGKYWDRFDDGTYRCVGCGAVLFDSDTKFDAGCGWPSFSSAIEEGRVERVVDT
                     SHGMRRVEVRCSNCGSHLGHVFDDGPEPTGERFCINSAAIDFDPAK"
     gene            complement(3980..>5194)
                     /locus_tag="VF453_07275"
     CDS             complement(3980..>5194)
                     /locus_tag="VF453_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEX7687489.1"
                     /translation="RALLLGEWRTADRADAPSFELQLKGAGRTPYSRMGDGRAVLRSS
                     IREFLCSEAMHALGVPTTRALAVAGGTLPVMRETPETGAIVTRVAPSFLRFGHYEHFA
                     HSGQHEALRRLVDFTIATYFPELAGGADGLDPLHAWLAEVVRRTAEMLARWQAVGFAH
                     GVMNTDNMSILGLTIDYGPFGFLDSFDPMHVCNHSDTHGRYAFARQPNVAWWNLHALA
                     HALRPLCDDPDATLPPVLDRFGDAYLAAYAARLREKFGLVETRDEDQALVEDFLNLLA
                     SEGTDYTIALRALAFFDRGAGAVPAELRDHFVDRAAIDLWGARYLARLEHEARTDADR
                     QAAMRAANPKFVLRNHLAQRAIEQAQAGDFEEVRRLHELLRRPFDEQPGRDAYAALPP
                     DWARHIEVSCSS"
BASE COUNT          747 a         1701 c         1898 g          848 t
ORIGIN      
        1 ggccgcggag atgctgggcc gcgtcggcct cggcgcgcgg ctcgggcact acccgcgcgt
       61 gctctcgggc ggcgagcagc agcgcgtggc gctcgcgcgc gccttcgtcg tgcggccgca
      121 ggtgctgctg gccgacgagc ccaccggcag cctcgacttc gccaccggcg agacggtgat
      181 gcagctgatg ttcgacctca accgcgaggt cggcacgacg ctggtgctgg tcacccacga
      241 ccgcgcgatc gccgcgcgct gcgaccgcca gctgcacatc gaggccggcc gcctggtcga
      301 gcccgagccg gcctgaacgg gtcgtcgagg cggggtgtcc gggggtgaga caaatccagg
      361 ggtgccggcg cgatagacgc cgatggcgga agaggccggg cgtccctggg tggcgctgga
      421 cggaagcgac cttgcgtcag gcgcaatcgg ccgccgcggc cgcgaatccg cctgatgcgt
      481 ctggcgcctg gcgccggaga ccgccgaccg gcgcgaccgg ccgccaagcg acctggcggt
      541 cagggaagga aatcggcctt gactttgccg tcctcgccga tgaacttgtc gcacagctcc
      601 ttgtgcacgt agcggagata catggcgtcg tcgccgctgg ggccggggtc ttcctcatcg
      661 gcggccagat gcttggcgta gtgctccatc gcgttgagca ccaggatgaa cgcccgcttg
      721 ggcatgtcga tttgcatgtc gagtccttat cctcggggtc cgtagatatc gctgccggcg
      781 tagtcgaagc cgaggtgctc gcccaccagt gaaacgaatt cgccgacggt catcgagcgc
      841 gtcaccgtca gggtgcaatg gcccggcggg tgcccgtcgt agacgacttc gaggcccaca
      901 gggatcgttc tctccgctag cttgaaccag tggatctgcg aaacctcggt gtagccctcc
      961 tccttggcga gcttctccaa ataggccttt ccgaaggctc catcggggct gctgccgagc
     1021 tgggtaaagt tcccctgcac ggtctgctcg atgaagagcg acagcccgtc gccggggtcg
     1081 acggtggcgc gcgccaggtc gtcgccctcg atcttcccgg acttgccgcg aacgccgatg
     1141 tcggaccacc ggagatagat cgcgggattc ttcgggccat tgcccttgcg gaagcagtcg
     1201 ccgtcgatct tggcgcccgc ggcccacagg cggcagcctg gtgtcagtcc gcgggccgct
     1261 gcacgttctt tgacttccgt gtcgttgagt agctttccca aagcctgctc ccctatcgtt
     1321 ggcgaacttg tacgctaagt ggacgccggt gggcccgcaa gcccccacga aatggggatt
     1381 gagacgcggt ttgaaacaaa aaaagcgccc ggccgcgagg cggggcgctt tgcgttgcgc
     1441 gcatcgggcc gggcaggcgc ccggcgcggc atcacttctt ggccggggct ggggcgcctt
     1501 cgtcggcggc gaacttgtag tccgtcttgg ccgacttgcg cagctgctcc tggtactcct
     1561 ggagcttgtc ctgagtcagc ttctgcttga tgcgctcctt ggcttcgtcg aagctcggga
     1621 acttggcggc acgggtctcg tcgaggcgga tgatgtggta gccgtactgc gtcttgaccg
     1681 gcgtgtcggt catctggccg ggcttgagcg attccagcgc ggtcgcgaac tcgggcacgt
     1741 agttggtcgc cggcgcccag tccaggtcgc cgccgttggt ggcgctctgg gtgtccttcg
     1801 attccttctt cgccacgtcc tcgaacttgc tgccggcctt gatctgggcg atcagcttct
     1861 tcgcgtcctc ttccttgtcg accaggatgt ggtgggcgtg gtactcgttg ccctgcgccg
     1921 ccttcagctt gtcgtactcc gccttcgcct cggcgtccga caccgggtgc gacttcgcgt
     1981 agttctggaa cagcgtgttg atcagcacgg tctggcgggt cagctcgagc tggctcttgt
     2041 actcgggcgt gttcatcacg ccggccttct cggcggcctg gctcaggatc tcgcgcatca
     2101 cgacctggtc gcgcgcctgg gtcagcatct ccggcggggc ctggtcgggc tggccatggg
     2161 tggccatgtt gaccagcgcg tccacgcgcg acttgggcac cggcttgccg ttgaccgtgg
     2221 cgatgttctg ggccatcact gccatcggca gcacgagggt gccgatcagg gcggcgaggg
     2281 ttttggaatt catgctgggg gactttcgag caaaacggtt ttcgaagaag gcagtgccaa
     2341 aaaggttcct ggggattcca ctaggaaatc cgcgcgcttc agtttcgtga aaccgggagt
     2401 aaccgaccgc acgcccgccc gccatcgtcc gagtttccgg aaaatgtccg gaacgcttcg
     2461 gcaggaaatc ccggcagggt ggcgcgttca gccctcggag ggcgcgcgcg cgtcgatggc
     2521 cagagcgtgc acgccgtcgg ccatccaggg gcgcagggca tcatacacga ggcgatggcg
     2581 cgcgacccgg gccagcccgg cgaagcgttc gctgacgatg cgcaggttgt agtgcgcccc
     2641 ttcgcgcgcg ccggcgtggc ccgcgtgggc ggcgctgtcg tcgacgagtt ccagcgcgga
     2701 cggcgcgagc gcctcctgga gcgcgcgcgt catcgcggcg tgcagctgcg ggcgggtgag
     2761 ggtggccatg gcggggcgct caggacgaac gcgactcgtc ggtcgccgcg ggcggcgccg
     2821 cgtgatcgag gtggcggctc atgtagacgc ccacgccgag gacgaagacc accatcagcc
     2881 cggtcgtgcc gaaggtgtgg aacttcgccc aggcgtcggt cgagaacgaa tagacgaccc
     2941 agacgttgac caggcccatc agcccgaaga acgcgacgaa caggaagttc atgcgccgcc
     3001 agacggcgtc cggcaggtcg atctcggcgc ccagcgtctg gcgcagcagg ttgtggccga
     3061 acagcgtctg gctggaccac agcgccaggc ccatcagcca gtagacgacg ctgggcttcc
     3121 acttgatgaa ggtctcgttg tgcagccaga cggtgatgcc gcccagcacg aggatcagcg
     3181 tcaggttgat ccacagcagc aggtcgatgc ggcgccggtt cgccttcatc cagaccacct
     3241 gcagcagcgt cgccgcggcg acgaccaccg tcgcgagcag caccggcgcc tccttcgggc
     3301 cgacgacgcc gccctgcacg agcgcgccca gccagtgcgt ggcgaactgg gcggaagcgt
     3361 cgggatgcag gccggcgacc ttgtaggtca ggaagaagaa ggcgagcggc aggaagtcga
     3421 gcaggatttt catcgtcggc tgcggagggc gcgcacccta cttcgcgggg tcgaagtcta
     3481 tcgcagcgga gttgatgcag aagcgctcgc cggtcggctc ggggccgtcg tcgaacacgt
     3541 ggcccaggtg cgagccgcag ttcgagcacc ggacctcgac ccgtcgcatg ccgtgcgagg
     3601 tgtcgacgac gcgctcgacc cggccttcct cgatcgccga cgagaagctc ggccagccgc
     3661 agccggcgtc gaacttggtg tcggagtcga acagcaccgc gccgcagccg acgcagcggt
     3721 aggtgccgtc gtcgaagcga tcccagtact tgccggtgaa ggcgcgctcg gtcgcggcgt
     3781 ggcgggcgac ctggtattgc atcgggtcga gcttcgcccg ccattcctcg tcggaccggc
     3841 gcaccgggta ggtcgcgtcg tcgtgggggt gcgggttcgt gaggcggggc ggggtggcgg
     3901 tcatggcttc cttcgtgggg ccggatgggc cggattcaag cgcgcgcccg ggtgggacgc
     3961 gcgcggtcgg ggacgggact caggacgagc aggagacctc gatgtgccgc gcccagtccg
     4021 gcggcagcgc ggcgtaggcg tcgcggccgg gctgctcgtc gaacgggcgc cgcagcagct
     4081 cgtgcaggcg gcgcacctcc tcgaagtcgc cggcctgcgc ctgctcgatg gcgcgctggg
     4141 cgaggtggtt gcgcagcacg aacttcgggt tcgcggcgcg catcgcggcc tggcgatcgg
     4201 cgtcggtgcg ggcctcgtgc tccagccgcg ccaggtagcg cgccccccag aggtcgatcg
     4261 cggcgcggtc gacgaagtgg tcgcgcagct cggcgggcac cgcgccggca ccgcggtcga
     4321 agaaggcgag cgcgcgcaag gcgatcgtgt agtcggtgcc ctcgctcgcg agcaggttca
     4381 ggaagtcctc gaccagcgcc tggtcctcgt cgcgcgtctc gacgagcccg aacttctcgc
     4441 gcaggcgcgc ggcgtacgcg gcgaggtagg cgtcgccgaa gcgatcgagc accggcggca
     4501 gcgtggcgtc gggatcgtcg cacagcggcc gcagcgcatg ggcgagcgcg tgcaggttcc
     4561 accaggcgac gttgggctgg cgcgcgaagg cgtagcggcc gtgggtgtcg gagtggttgc
     4621 agacgtgcat cgggtcgaac gagtcgagga agccgaacgg gccgtagtcg atcgtcagcc
     4681 cgaggatgga catgttgtcc gtgttcatca ccccgtgcgc gaagccgacg gcctgccatc
     4741 gcgcgagcat ctcggcggtg cggcgcacga cctccgcgag ccaggcgtgc agcgggtcga
     4801 ggccgtccgc gccgcccgcc agctcgggga agtacgtcgc gatcgtgaag tcgaccagcc
     4861 gccgcagcgc ctcgtgctgg ccgctgtggg cgaagtgctc gtagtggccg aagcgcagga
     4921 agctgggcgc gacgcgcgtg acgatggcgc cggtctcggg cgtctcgcgc atcaccggca
     4981 gcgtgccgcc ggccaccgcc agcgcgcgcg tcgtcggcac gcccagcgcg tgcatcgcct
     5041 ccgagcagag gaactcgcgg atcgacgagc gcagcaccgc gcgcccgtcg cccatgcgcg
     5101 agtagggcgt gcggccggcg cccttgagct ggagctcgaa gctcggcgcg tcggcgcggt
     5161 cggcggtgcg ccactcgccc agcagcagcg cgcg
//
LOCUS       DASVLN010000298         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_177058, whole genome shotgun sequence.
ACCESSION   DASVLN010000298 DASVLN010000000
VERSION     DASVLN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177058"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF453_07280"
     CDS             complement(<1..243)
                     /locus_tag="VF453_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7687490.1"
                     /translation="MSHADPPSSCDVLVIGAGPAGAACALWLARAGADVALVDQHDFP
                     REKVCGDALIPDAHHALRRLGLHDEVMAVAQPAGHVA"
     gene            complement(284..>1801)
                     /locus_tag="VF453_07285"
     CDS             complement(284..>1801)
                     /locus_tag="VF453_07285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7687491.1"
                     /translation="ARSMLDVARRAADRQREALSLALSGRIADANLDTAAANRQLAQA
                     ERLARLDGSPWVEVRVTILSGLVHSRHNQLAATRRDYESALAIAQAAGLGRESLTLRA
                     NLSDVYIREGRPRDALAAIRLALPVARLHHDLRSERLLLHNQTLAKLALGRLAEAKAD
                     MLHVLELWKRDTGPGQQQRALREFGDALARAGDVAGALELFHREDALARQIQAANKSA
                     AEAELRARYDQESQKRRIELLARDNQLKTAQLENQAIANRLWMVAGVAMGLLGVLAVL
                     MYLRMRELNRTLVRHEALLRAHSERDALTGLANRRQFREVMRLRGGERAFRGALLMVD
                     VDHFKRVNDQHGHATGDRVLVEVARRLGAAMRADDLVARWGGEEFLAFAPHVEGAELD
                     QLAERVRVAMAQAPVALDNGQALEITVSIGYAAFPLPESRVEVNWEQAVNLADLSLYT
                     AKNQGRNCAVGIVRTTAGTAAALREVEADIAQARADGRVTMKVSPEAAPREAVGP"
BASE COUNT          209 a          650 c          704 g          238 t
ORIGIN      
        1 ggcgacgtgg ccggccggct gggcgaccgc catgacctcg tcgtgcagcc cgaggcggcg
       61 cagcgcgtgg tgggcgtccg ggatcagcgc gtcgccgcag accttctcgc gcgggaagtc
      121 gtgctggtcg acgagcgcga cgtcggcgcc cgcgcgcgcg agccacagcg cgcaggcggc
      181 cccggccggg ccggcgccga tcaccaggac atcgcaggag gagggcgggt cggcatggga
      241 catgggccgc gattgtaggg agcgcggccg cggcggcggg ccgtcagggg ccgaccgcct
      301 ctcgcggcgc ggcctcgggg ctcaccttca tcgtgacacg gccgtccgcg cgcgcctggg
      361 cgatgtcggc ctcgacctcg cgcagcgccg cggcggtgcc ggcggtggtg cgcacgatgc
      421 cgaccgcgca gttgcggccc tggttcttgg cggtgtagag cgacaggtcg gccaggttga
      481 cggcctgctc ccagttcacc tcgacgcggc tctccggcag cgggaaggcg gcgtagccga
      541 tcgagacggt gatctccagc gcctgcccgt tgtccagcgc gaccggcgcc tgcgccatcg
      601 cgacacgcac gcgctcggcg agctggtcga gctcggcgcc ctcgacgtgc ggcgcgaagg
      661 cgaggaattc ctccccgccc cagcgcgcga cgagatcgtc cgcccgcatc gccgcgccga
      721 gccggcgcgc gacctccacc agcacgcggt cgccggtggc gtggccgtgc tggtcgttga
      781 cgcgcttgaa atggtcgacg tccaccatca gcagcgcgcc gcggaaggcg cgctcgccgc
      841 cgcgcagccg catgacctcg cggaactggc gccggttggc caggccggtg agcgcgtcgc
      901 gctcgctgtg cgcgcgcagc agcgcctcgt gcctgacgag cgtgcggttg agctcgcgca
      961 tgcgcaggta catcaggacg gccagcacgc ccagcaggcc catcgccacg ccggcgacca
     1021 tccacagccg gttggcgatc gcctggttct cgagctgcgc ggtcttgagc tggttgtcgc
     1081 gcgcgagcag ctcgatgcgc cgcttctgcg actcctggtc gtagcgcgcg cgcagctcgg
     1141 cctcggcggc cgacttgttg gcggcctgga tctgccgggc gagcgcatcc tcgcggtgga
     1201 acagctccag cgcgccggcg acgtcgccgg ccctggcgag ggcgtcgccg aactcccgca
     1261 gcgcgcgctg ctgctggccg ggtccggtgt cgcgcttcca cagctccagg acgtgcagca
     1321 tgtcggcctt ggcctcggcc aggcggccca gcgcgagctt ggccagcgtc tggttgtgca
     1381 gcagcagccg ctcggagcgc aggtcgtggt gcagccgcgc gaccggcagc gcgagcctga
     1441 tggcggccag cgcgtcgcgc ggacggccct cgcggatgta gacgtcgctc aggttggcgc
     1501 gcagcgtcag cgactcgcgg ccgaggccgg cggcctgggc gatcgccagc gccgactcgt
     1561 agtcgcgccg ggtcgccgcg agctggttgt ggcggctgtg cacgaggccc gacaggatcg
     1621 tcacgcgcac ctcgacccac ggcgagccgt ccaggcgcgc gagccgctcg gcctgggcga
     1681 gctggcgatt ggcggcggcg gtgtcgaggt tggcgtcggc gatgcggccg gacagggcca
     1741 gcgagagcgc ctcgcgctgg cggtcggcgg cgcggcgcgc gacgtcgagc atgctgcgcg
     1801 c
//
LOCUS       DASVLN010000299         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_500167, whole genome shotgun sequence.
ACCESSION   DASVLN010000299 DASVLN010000000
VERSION     DASVLN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500167"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VF453_07290"
     CDS             <1..226
                     /locus_tag="VF453_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7687492.1"
                     /translation="AAAAGVQAEAHLVDGEAIDLPQTVAQRVGETGVDLVVMGTRGRD
                     GLARTLLGSVAEEIQRRSPVPVLLVRTPEE"
     gene            320..481
                     /locus_tag="VF453_07295"
     CDS             320..481
                     /locus_tag="VF453_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687493.1"
                     /translation="MNDSTKRTIMARSAVGDIPILPVLPEAIAVLDDGLRFGHAERVT
                     AGAAELAPA"
     gene            511..1506
                     /locus_tag="VF453_07300"
     CDS             511..1506
                     /locus_tag="VF453_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011853744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX7687494.1"
                     /translation="MRAMVLEQPATPLVERVRPEPQPGPVEVGLRVLACAVCRTDLHV
                     VDGDLPWRGPVVPGHEIVGVVEAVGLAVRWPDVGERVGVAWLGGTCGRCEFCRRDREN
                     LCDTPEFTGYTRDGGFATHVIANAAYCVALPGLRPGMADDLHTAPLLCAGLIGWRALK
                     AAGDSTIDLGLYGFGAAAHLIVQVATRRGMRVRAFTRRGDEAAQALARRMGAVWAGAS
                     DEPSPVPLDAAIIFAPAGELVPLALAAVRKGGRVVCGGIYMSDVPSFPYERLWGERSL
                     VSVANLTRRDAREFMGIAASMPLEVTATAYPLARANEALADLREGRVVGAAVLVP"
     gene            complement(1526..2176)
                     /locus_tag="VF453_07305"
     CDS             complement(1526..2176)
                     /locus_tag="VF453_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7687495.1"
                     /translation="MKSDDQLQAQVIDELAWDPAVHHTDIGVAVRDGVVTLNGCVRSL
                     AEKHAAEDAVRRVAGVKGLAVALEVDLLPASERSDSEIAFAANHALEWHALVPVDAVQ
                     VTVERGHVTLTGQVAWHYQRAAAERTVQRLVGVRGVTNQVHVKPPVARDRIEQGIHEA
                     LSRRADRSAQHVQVRVHGSRVTLSGSVHSWGERDAVCGAAWSAPGVTSVVDEMVVG"
     gene            complement(2214..2588)
                     /locus_tag="VF453_07310"
     CDS             complement(2214..2588)
                     /locus_tag="VF453_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX7687496.1"
                     /translation="MNIETQEHLPMLRQLLLFRMHELEAEARAAAVDRASSATEVDMA
                     MVIDRKDEADAEQRDALAEQVERRSRDELARCREALKRLDDGTYGDCRDCGMPIPLAR
                     LMAQPQAERCAPCQAEIERQAH"
     gene            2852..3271
                     /locus_tag="VF453_07315"
     CDS             2852..3271
                     /locus_tag="VF453_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7687497.1"
                     /translation="MKVLLAVDGSDYTRRALDYLASHAWLRSGNQLTAFTVVLPVPHR
                     AAAFVPSQVVQNYYRDDAEQVLRPVRERLAGADAEVDSDYAVGRRAEAIAHKATSGGY
                     ELVVMGSHGHGALANVVLGSVATRVLALCSTPVLLIR"
     gene            3298..>3485
                     /locus_tag="VF453_07320"
     CDS             3298..>3485
                     /locus_tag="VF453_07320"
                     /inference="COORDINATES: protein motif:HMM:NF016211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HEX7687498.1"
                     /translation="MSRLVFRILAVAALLLAALAAVLPVVPAIPFLLLAAAAAARGWP
                     WLEMKLAAHPRIGPALAA"
BASE COUNT          404 a         1275 c         1314 g          492 t
ORIGIN      
        1 cgccgcggcc gccggcgtgc aggccgaggc gcaccttgtc gacggcgagg ccatcgacct
       61 gccgcagacg gttgcgcaac gcgtcggcga gaccggggtc gacctggtgg tgatgggcac
      121 gcgcggacgc gacggcctgg cccgtaccct gctcggcagc gtcgccgagg agatccagcg
      181 gcgctcgccc gtccccgtgc tgctcgtgcg cacgccggag gagtaggtcg cacgccgccg
      241 ccgacgagag gcaagcggcg gcgtgcgtgc gacgaggcca tccatcgacg ggcgggcgcc
      301 cgccaatcgg gagagttcca tgaacgactc gacgaagcgc acgatcatgg cgaggtccgc
      361 ggtcggcgac atcccgatcc ttcccgtcct gccggaggcg atcgccgtgc tcgacgatgg
      421 cctgcggttc ggccacgcgg agcgggtgac ggccggcgcg gcggaactcg cgccggcgtg
      481 agccggtcgg cccgccagga gccgcatgcc atgcgcgcga tggtgctcga gcagcccgcg
      541 acgccgctgg tcgagcgcgt ccgcccggag ccgcagcccg gcccggtcga ggtcggcctg
      601 cgcgtgctcg cctgcgcggt ctgccgcacg gacctgcacg tggtcgacgg cgacctgcca
      661 tggcgcggcc ccgtcgtccc ggggcacgag atcgtcggcg tggtcgaggc ggtcgggctg
      721 gccgtccgct ggcccgacgt cggcgagcgc gtgggcgtcg cctggctggg cggcacctgc
      781 ggccggtgcg agttctgccg ccgggatcgc gagaacctgt gcgacacgcc cgagttcacc
      841 ggctacaccc gcgacggcgg cttcgccacc cacgtcatcg ccaacgcggc ctattgcgtc
      901 gcgctgcccg ggctgcggcc gggcatggcc gacgacctgc ataccgcgcc cctgctgtgc
      961 gcgggcctga tcggctggcg cgcgctgaag gcggcgggcg actccacgat cgacctcggc
     1021 ctgtacggct tcggcgcggc ggcccatctc atcgtgcagg tggcgacgcg gcgcggcatg
     1081 cgcgtgcgcg ccttcacgcg ccgcggcgac gaggccgcgc aggcgctggc gcgccgcatg
     1141 ggcgcggtgt gggcgggcgc gtcggacgag ccgtcgcccg tgccgctcga cgcggccatc
     1201 atcttcgccc ccgcgggcga gctcgtcccg ctggcgctgg cggccgtccg caagggcggg
     1261 cgcgtcgtct gcggcggcat ctacatgagc gacgtgccgt cgttcccgta cgagcgcctg
     1321 tggggcgagc gctcgctggt ctcggtcgcc aacctgacgc ggcgcgacgc ccgcgagttc
     1381 atgggcatcg ccgcctcgat gccgctggag gtcacggcga cggcctatcc gctcgcccgc
     1441 gccaacgagg cgctcgcgga cctgcgcgag ggtcgcgtcg tcggcgcggc cgtcctcgtc
     1501 ccgtgatccg tccggtggcg ggcgttcagc cgacgaccat ctcgtcgacc acggacgtga
     1561 cgccgggcgc cgaccaggcc gcgccgcaga ccgcatcgcg ctcgccccac gagtgcacgc
     1621 tgcccgacag cgtcaccctc gagccgtgca cccgcacctg cacgtgctgc gcggagcgat
     1681 cggcgcggcg cgagagcgcc tcgtggatgc cctgctcgat ccggtcgcgc gccacgggcg
     1741 gcttcacgtg cacctggttg gtcacgccgc ggacgccgac cagccgctgc accgtgcgct
     1801 cggccgcggc gcgctggtag tgccaggcga cctggcccgt gagcgtcacg tggccgcgct
     1861 cgaccgtgac ctgcacggcg tcgacaggca ccagggcgtg ccactcgagg gcgtggttcg
     1921 cggcgaaggc gatctcggaa tcgctgcgct cgctggccgg cagcaggtcg acctcgaggg
     1981 cgaccgcaag ccccttgacg ccggcgaccc ggcgcacggc atcctcggcc gcatgcttct
     2041 cggcgaggct gcgcacgcac ccgttgaggg tcacgacgcc gtcgcggacg gcgacgccga
     2101 tgtccgtgtg gtgcacggcg ggatcccagg ccagctcgtc aatgacttgg gcctgcagtt
     2161 ggtcatcgga tttcatgatc gctcctgggg gaaggcgagg gcaggactgc cgctcagtgg
     2221 gcttgccgct cgatctccgc ctggcagggc gcgcagcgct cggcctgggg ctgtgccatg
     2281 aggcgcgcga gcgggatggg catgccgcaa tcgcggcagt cgccgtaggt gccgtcgtcg
     2341 agccgcttca gcgcctcgcg gcagcgggcg agctcgtcgc ggctgcgacg ctcgacctgc
     2401 tcggcgagcg cgtcgcgctg ctcggcgtcg gcctcgtcct tgcggtcgat caccatcgcc
     2461 atgtcgacct cggtggcgct gctcgcgcgg tcgacggccg cagcgcgggc ctcggcctcg
     2521 agctcgtgca tgcgaaacag cagcagctgg cgcagcatcg gaaggtgttc ctgggtctcg
     2581 atgttcatgg cggctcgtcg tcgaaggtcg gtggatggcg tggagcatcc cgccgcgggg
     2641 ggagatcccg gttgaggcat ctcaagcgcg gcgcggctcg cggcggccct cgccatcgac
     2701 ccgcactcta ggggcgggcg gccgtgcgca cttgagcgcg cgcaatcgtc ggccaaggcg
     2761 gagcttgcgc tcgcgcaagg gggcggccgt ccggggcggt agcctcaccg aggcctgcgg
     2821 cgcctggagc cgccaacctg ggagatccgg catgaaagtc ctcctcgccg tcgacggcag
     2881 cgactacacg cggcgggccc tcgactatct cgcgagccat gcatggctga ggtcgggcaa
     2941 ccagctcacg gcgttcacgg tggtgctccc ggtgccgcat cgagccgccg ccttcgtgcc
     3001 ttcgcaggtg gtgcagaact actatcgcga cgacgcggaa caggtgctgc gcccggttcg
     3061 cgagcgcctg gccggcgcgg acgccgaggt cgacagcgac tacgccgtgg gacgccgcgc
     3121 cgaggccatc gcgcacaagg cgacctcggg cggctacgag ctggtcgtga tgggatcgca
     3181 cggccacggc gcgctcgcca acgtcgtcct cggctcggtg gcgacgcgcg tgctggccct
     3241 gtgcagcacg ccggtgctgc tgatccggtg acggcgtcgc cgctgcggcc gggtgccatg
     3301 tcccgcctcg tgttcaggat cctggcggtc gccgcgctgc tgctcgcggc gctggcggcc
     3361 gtcctgccgg tcgtccccgc catcccgttc ctcttgctcg ccgccgcggc cgcggcgcgc
     3421 ggctggccgt ggctcgagat gaagctggcc gcgcatccgc gcatcggccc ggccctcgcg
     3481 gcctg
//
LOCUS       DASVLN010000300         5570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_284634, whole genome shotgun sequence.
ACCESSION   DASVLN010000300 DASVLN010000000
VERSION     DASVLN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284634"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1339
                     /locus_tag="VF453_07325"
     CDS             101..1339
                     /locus_tag="VF453_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEX7687499.1"
                     /translation="MVEPENDNDDAHSPVLAQALQTLRTWPWFDTLRILRLRFRDDRL
                     GLTASSLTFTTLISLVPLLTVMLAIFTAFPMFGTFQGGLEKYFLQTLVPPTIAKPVLG
                     ALTTFAAKASRLGAFGLIALGFTAMALMLTIDRTLNAIWRVRKPRPIAQRVLIYWAAV
                     TLGPLILGASLSLTSYAISASAGLVGKMNGGVSFSLGLVEFAVLATAMSALFFYVPNA
                     PVASKHAAAGGLFVAAGFEIAKRALAWYLQAVPTYSSIYGAFATVPIFLLWIYLGWII
                     VLLGAVVAAYAPSLQLRLVHQVRRPGQRFALALEIVHRLLESRRAGEHGLTGVELAGR
                     LRVDPLQIESVLEALQALDWVGRLAEDGASRYVLLCDPLHTALLPLVDTVLLAPIDAL
                     APARALMQVDRIRLSDVLPA"
     gene            1350..1997
                     /locus_tag="VF453_07330"
     CDS             1350..1997
                     /locus_tag="VF453_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687500.1"
                     /translation="MHTPGAFSRSFAPPPARARWWLAIGVLLAVPLLALAVDVGWRML
                     RWNAELKGVRFIDHPEKIRSDNAAVTVSGYLVATVASDVDFFALARKESAQPKVEATL
                     CESDRPLDNWYDPLPRESQGGPRPYRYAVLVPLRGRDVDLARTNEEVCLRFRAATNNP
                     LSWVRSRPVVVRLAPALREELAAYGRRGAPVELTLDPGCAPHLCEPGFVESRLRP"
     gene            complement(2047..2628)
                     /locus_tag="VF453_07335"
     CDS             complement(2047..2628)
                     /locus_tag="VF453_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX7687501.1"
                     /translation="MMARNLSVEPPRVGNTLAELRQSRALSLDELSRLAGVSKSMLSQ
                     IERNQANPTVAVVWRLANALGVPLGQLLDGERPAAPAITTVAGHATPSLRSPDGKCEL
                     RILGPIELAGQFEWYELTVQPGGRLESQAHEPGSREHLSVLGAGTLEVRSGSDVSLLH
                     GDETARYAVDVPHAICNPGGEPVKALMVVVHNA"
     gene            2758..3969
                     /gene="kbl"
                     /locus_tag="VF453_07340"
     CDS             2758..3969
                     /gene="kbl"
                     /locus_tag="VF453_07340"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX7687502.1"
                     /translation="MTSTDRFYQHCRAELEGIREAGLFKGERIIATPQGAMVRLADGR
                     ELINLCANNYLGLSSHPKVIEAAHEALRTHGYGMSSVRFICGTQDIHKELEARLAAFL
                     GTEDTILYAAAFDANGGLFEPLLGEEDAIISDALNHASIIDGIRLCKARRFRYAHNDM
                     ADLEVQLQAADAAGARFKLVFTDGAFSMDGTIAQLDKIRALCDRYGALLGIDECHATG
                     FLGTRGRGTHEHRGVFGKIDIITGTLGKALGGASGGFTTGRREVIELLRQRSRPYLFS
                     NTVMPAIVGGSLAVLDLLEASTELRDKLADNTAWFRQAITAAGFEIKPGEHPIVPIMI
                     GDAVKAQALSQRILELGLYAVGFFYPVVPKGQARIRVQMSAVHDRHHLEKAVEVFAKA
                     GKELGLVGAPR"
     gene            3966..4931
                     /locus_tag="VF453_07345"
     CDS             3966..4931
                     /locus_tag="VF453_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7687503.1"
                     /translation="MSRPARILVIGANGQIGSELAGALSKRPGVEAVITSDVAPVGRT
                     PGVPHEQLDVTDAAALAAVVERHHVTQVYLLAAALSANGEKHPQWAWNLNMVGLLNVL
                     ELARTHKLDKVFWPSSIAAFGPTTPADATPQATVMDPTTIYGISKLAGERWCAWYHAN
                     HGVDVRSLRYPGLISWKTPPGGGTTDYAVEIFHAALRDGRYTCFLEEHQALPMMLMDD
                     ALRATIELMEAPAEAIRERGSYNLSAISFTPREIADAIARRIPGFTMDCKPDFRQAIA
                     ANWPRSIDDSAARRDWGWQARFGLDEMVDEMLTQLAPLVKAKAAA"
     gene            complement(5042..5371)
                     /locus_tag="VF453_07350"
     CDS             complement(5042..5371)
                     /locus_tag="VF453_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEX7687504.1"
                     /translation="MADTTLPTDPKTPHSFAEFYPVYLSEHRNLTCRRLHFVGSTLAL
                     LCLWCLAWTREPGFIVAALVCGYGFAWVGHFAFEKNRPATFRRPLWSFMGDWRMYFDI
                     WMHRIPF"
     gene            complement(5421..>5570)
                     /locus_tag="VF453_07355"
     CDS             complement(5421..>5570)
                     /locus_tag="VF453_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7687505.1"
                     /translation="ERDAMTPPKAAAALADALRARVTRLRAGHALMQEAPDALLAALR
                     QAVVP"
BASE COUNT          789 a         1992 c         1950 g          839 t
ORIGIN      
        1 gcgcccgcgt cgcgccgtcg gccgctcgcg gcggcagctg cgcggccggg gccggtgtcg
       61 ggggactcac gggatcggac atgtttggca tgatagtcgg atggtggaac ccgagaacga
      121 caacgacgac gcgcattcgc ccgtgctggc gcaggcgctg cagaccctgc gtacctggcc
      181 ctggttcgac acgctgcgca tcctgcgcct gcgtttccgc gacgatcgcc tcggcctgac
      241 cgcgagcagc ctgaccttca cgacgctcat ctcgctggtg ccgctgctga cggtgatgct
      301 ggcgatcttc accgccttcc cgatgttcgg cacgttccag ggcggcctgg agaagtactt
      361 cctgcagacg ctcgtgccgc cgacgatcgc caagcccgtg ctgggcgcgc tcacgacctt
      421 cgccgcgaag gcgagccggc tcggtgcctt cggcctgatc gcgctcggct tcaccgcgat
      481 ggcgctgatg ctcacgatcg accgcaccct caacgcgatc tggcgcgtgc gcaagccgcg
      541 gccgatcgcg cagcgggtgc tgatctactg ggccgcggtg acgctcgggc cgctgatcct
      601 cggtgcgagc ctgtcgctca cgtcgtacgc gatctcggct tccgcgggcc tggtgggcaa
      661 gatgaacggc ggcgtgagct tctcgctcgg gctggtggag ttcgcggtgc tggccaccgc
      721 catgagcgcg ctgttcttct acgtgcccaa cgcgcccgtc gcctccaagc acgcggccgc
      781 gggcgggctg ttcgtcgccg cgggcttcga gatcgccaag cgcgccctcg cgtggtacct
      841 gcaggcggtg ccgacctact cgagcatcta cggcgccttc gcgacggtgc cgatcttcct
      901 gctgtggatc tacctcggct ggatcatcgt gctgctcggg gcggtcgtcg ccgcgtatgc
      961 gcccagcctg caactgcggc tcgtgcacca ggtgcgtcgc cccggccaac gcttcgcgct
     1021 cgcgctggag atcgtgcacc ggctgctgga gtcgcgccgc gccggcgagc acggcctgac
     1081 cggcgtcgag ctcgccgggc gcctgcgcgt cgacccgctg cagatcgagt cggtgctgga
     1141 ggcgctgcag gcgctcgact gggtcggccg cctcgccgag gacggcgcgt cgcgctacgt
     1201 gctgctgtgc gacccgctgc acacggcgct cctgccgctg gtcgataccg tgctgctcgc
     1261 gcccatcgac gcgctcgccc ccgcccgcgc gctgatgcag gtcgaccgca tccggctgtc
     1321 cgacgtcctg cccgcctgac cgatcctcca tgcacacgcc cggcgccttc tcgcgttcct
     1381 tcgcaccgcc gcccgcgcgt gcgcgctggt ggctggcgat cggcgtgctg ctcgccgtgc
     1441 cgctgctggc gctggcggtc gacgtcggct ggcgcatgct gcgctggaac gccgagttga
     1501 agggcgtgcg cttcatcgac catccggaga agatccgttc cgacaacgcc gcggtcaccg
     1561 tcagcggcta cctcgtcgcg accgtggcca gcgacgtcga cttcttcgcg cttgcccgca
     1621 aggagtcggc gcagcccaag gtcgaggcga cgctgtgcga gtccgaccgc ccgctggaca
     1681 actggtacga cccgctgccg cgcgagtccc agggcggccc gcggccctac cgctacgccg
     1741 tgctggtgcc gctgcggggc cgggatgtcg acctcgcgcg cacgaacgag gaggtctgcc
     1801 tgcgcttccg cgccgccacc aacaacccgc tcagctgggt gcgctcgcgg ccggtcgtcg
     1861 tgcggctcgc gccggcgttg cgcgaggagc tggccgccta tggccgccgc ggcgcgccgg
     1921 tggagctgac gctcgacccg ggctgcgcgc cgcacctttg cgagccgggc ttcgtcgaat
     1981 cgcgactcag gccttgatgc cgcaggccgt atacggcgtc gcgacctccg gatgtcgccg
     2041 tgccgttcag gcgttgtgca ccaccaccat cagcgccttc acgggctcgc cgccgggatt
     2101 gcagatggcg tgcggcacgt cgaccgcgta gcgcgccgtc tcgtcgccgt gcagcagcga
     2161 gacgtccgag cccgagcgca cctccagcgt gccggcgccc agcacgctca ggtgctcgcg
     2221 cgagccgggc tcgtgggcct gcgactcgag gcggccgccg ggctgcacgg tgagctcgta
     2281 ccactcgaac tggcccgcga gctcgatcgg cccgaggatg cgcagctcgc acttgccgtc
     2341 cgggctgcgc agcgagggcg tcgcatggcc ggcgaccgtc gtgatcgcgg gggcggcggg
     2401 gcgctcgccg tcgagcagct ggccgagcgg cacgcccagc gcgttggcca ggcgccagac
     2461 caccgcgacg gtggggttgg cctggttgcg ctcgatctgc gacagcatcg acttcgacac
     2521 ccccgccagc cgcgacagct cgtccagcga cagcgcgcgc gattggcgca gttcggccag
     2581 ggtattgccg acccgcggcg gttcgaccga gagattgcgc gccatcatcg tccgttggag
     2641 ggtgccgcgc cggcctcgct tgacgcgacc ggcattgttc aggatactgc attcttgttc
     2701 gatatatcga acttcgaatt ttgcattgca cggcattgtg cctgaaggac gaaacgcatg
     2761 accagcaccg accggttcta ccagcactgt cgcgccgagc tcgaggggat ccgcgaggcg
     2821 ggcctgttca agggcgagcg catcatcgcg acgccgcaag gcgcgatggt gcggctggcg
     2881 gacgggcgcg agctcatcaa cctgtgcgcc aacaactacc tcgggctgtc ctcgcacccg
     2941 aaggtgatcg aggcggcgca cgaggcgctg cgcacgcacg gctacggcat gagctcggtg
     3001 cgcttcatct gcggcacgca ggacatccac aaggagctcg aggcgcgcct ggccgccttc
     3061 ctcggcaccg aggacaccat cctctacgcc gccgccttcg acgccaacgg cggcctgttc
     3121 gagccgctgc tgggcgagga ggacgcgatc atcagcgacg cgctgaacca cgcctccatc
     3181 atcgacggca tccgcctgtg caaggccagg cgctttcgct atgcgcacaa cgacatggcc
     3241 gacctggagg tgcaactgca ggccgccgac gccgcgggcg cgcgcttcaa gctggtgttc
     3301 acggacggcg cgttctcgat ggacggcacg atcgcgcagc tcgacaagat ccgcgccctg
     3361 tgcgaccgct atggcgcgct gctgggcatc gacgagtgcc atgcgaccgg cttcctcggc
     3421 acgcgcggac gcggcacgca cgagcaccgc ggcgtcttcg gcaagatcga catcatcacg
     3481 ggcacgctgg gcaaggcgct cggcggcgcg tcgggcggct tcacgaccgg caggcgcgag
     3541 gtgatcgagc tgctgcgcca gcgctcgcgg ccctacctgt tctcgaacac ggtgatgccg
     3601 gcgatcgtgg gcggctcgct ggcggtgctc gacctgctgg aggccagcac cgagctgcgc
     3661 gacaagctcg ccgacaacac ggcctggttc cgccaggcca tcacggcggc gggcttcgag
     3721 atcaagccgg gcgagcaccc gatcgtgccc atcatgatcg gcgacgccgt gaaggcgcag
     3781 gcgctgtcgc agcgcatcct cgagctcggc ctgtacgcgg tcggcttctt ctaccccgtg
     3841 gtcccgaagg ggcaggcgcg catccgcgtg cagatgtccg cggtgcacga ccgccatcac
     3901 ctggagaagg cggtcgaggt cttcgccaag gccggcaagg agctgggcct cgtgggagcg
     3961 ccgcgatgag ccgccccgcg cgcatcctcg tcatcggcgc gaacggccag atcggctccg
     4021 agctggcggg cgcgctgtcg aagcgtcccg gcgtcgaagc cgtcatcacg agcgacgtcg
     4081 cgccggtcgg ccgcacgccc ggcgtgccgc acgagcagct ggacgtgacc gacgccgccg
     4141 cactggccgc ggtggtcgag cgccaccatg tcacgcaggt ctacctgctc gcggcggcgc
     4201 tgtcggccaa cggcgagaag catccgcagt gggcctggaa cctgaacatg gtcggcctgc
     4261 tgaacgtgct ggagctcgcg cgcacgcaca agctcgacaa ggtcttctgg cccagctcga
     4321 tcgccgcctt cggcccgacg acgccggcag acgccacgcc gcaggcgacg gtgatggatc
     4381 cgacgacgat ctatggcatc agcaagctcg ccggcgagcg ctggtgcgcc tggtatcacg
     4441 ccaaccacgg cgtggacgtg cgcagcctgc gctacccggg cctgatcagc tggaagacgc
     4501 cgccgggcgg cggcacgacc gactacgccg tcgagatctt ccacgccgcg ctgcgcgacg
     4561 gccgctacac ctgtttcctc gaagagcacc aggccctgcc gatgatgctg atggacgatg
     4621 ccctgcgcgc caccatcgag ctgatggagg ccccggccga ggcgatcagg gagcgcggca
     4681 gctacaacct gtcggcgatc agcttcaccc cgcgcgagat cgccgacgcg atcgcccggc
     4741 gcatcccggg cttcacgatg gactgcaagc ccgacttccg ccaggccatc gccgccaact
     4801 ggccgcgttc gatcgacgac agcgcggcgc gccgggactg gggctggcag gcccgcttcg
     4861 ggctcgacga gatggtcgac gagatgctca cgcagctcgc gcccctcgtg aaggcgaagg
     4921 cggcggcctg agagacgccg cacaccgcaa gcgaagaagg ccgcgacatg cggccttcgt
     4981 catttgcggg aaaggttcgg gcccgccggt cggcgcctgg gcggacgccg gcgcgccgac
     5041 gtcagaacgg gatgcgatgc atccagatgt cgaagtacat ccgccagtcg cccatgaagc
     5101 tccacagcgg ccgccggaag gtggcggggc ggttcttctc gaacgcgaag tggccgaccc
     5161 atgcgaagcc gtagccgcac accagcgccg cgacgatgaa gcccggctcg cgagtccagg
     5221 ccaggcacca caggcacagc agcgccagcg tcgagccgac gaagtgcagg cggcgacagg
     5281 tcaggttgcg gtgttccgac aggtagaccg gatagaactc cgcgaagctg tgcggagtct
     5341 tcgggtcggt gggcagcgtc gtgtcggcca tgtcgttctc cggcatgtcg cgggcccggg
     5401 gggcgtcgcg ttttcgttct tcaggggacg acagcttgcc gcagcgcggc cagcagcgca
     5461 tcgggggctt cctgcatcag cgcgtgcccg gcgcgcaggc gcgtcacgcg ggcgcgaagg
     5521 gcgtccgcga gtgccgcggc ggccttcggc ggcgtcatcg cgtcgcgctc
//
LOCUS       DASVLN010000301        14254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_177119, whole genome shotgun sequence.
ACCESSION   DASVLN010000301 DASVLN010000000
VERSION     DASVLN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14254
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177119"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VF453_07360"
     CDS             complement(<1..878)
                     /locus_tag="VF453_07360"
                     /inference="COORDINATES: protein motif:HMM:NF028473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687506.1"
                     /translation="MSRAALPPAARVAAALATAMLLAAGPAHAGSTTVRDADGSQWSI
                     QDDPADDARDFLMRHLDRAGQPVAGFGRDGFAHFTVATTNDPPASVRVDASHRIWMVG
                     ASLAGNQPQPVVARFLPDGTPDLRWGVQGKVQLTPAGVPVQPNDLLPLADGSVLVAGE
                     VTGGIAPQAIVYHLRPDGALDLDFGTHGLWRREGANEASTATSLAANPAGLVVVAVAV
                     RGEKPAQELWTFNDVPPRLVNKGPLDDSSDGEDLRVDWAGQGWQTGRNGGPTPVVPAA
                     LLTNRPAAEAPSRVPAA"
     gene            complement(875..2353)
                     /locus_tag="VF453_07365"
     CDS             complement(875..2353)
                     /locus_tag="VF453_07365"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7687507.1"
                     /translation="MDMKTSPIATLNDPGLLKTDALVGGEWVAGGARFDVVDPATGQK
                     LADVANLAGADALAAIAAADKAWPLWRAKTAKERHAVMMKWFQLIVANADDLARILTA
                     EQGKPIAEARGEVVYGASFIEWFAEEAKRVYGETIPTTDNGKRYLVLKQPVGVCAAIT
                     PWNFPIAMITRKVAPALAAGCPVVIKPAEQTPLSALALAELAQRAGMPAGVLNVVTAD
                     AANSILVGNELCASEVVRHLSFTGSTEVGRILMKQCAPTIKKLSLELGGNAPFLVFDD
                     ADIDSAVEGAMISKYRNAGQTCVCANRLYVQDGVYDTFIARLAEKARGIKVGNGFEPG
                     VNQGPMIDANAIAKVEAHIEDALAKGARVVVGGARIDERFFTPTVLADVTPEMIVTRE
                     ETFGPLAPVVRFKTESEAIALANATEFGLAAYFYSRDVGRIFRVAEALESGMVGINTG
                     MISVAEVPFGGVKQSGLGREGARQGIEDYVEVKYLCLGDIQK"
     gene            complement(2557..3849)
                     /gene="gabT"
                     /locus_tag="VF453_07370"
     CDS             complement(2557..3849)
                     /gene="gabT"
                     /locus_tag="VF453_07370"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX7687508.1"
                     /translation="MQRDAHLNNAALMARRRAALPSGLGQTYDIVCERAENAEVWDVE
                     GKRYIDFAGGIAVLNTGHRHPKIVAAVKAQLDKVHHTCFQVLAYEPYIELAERLIAKT
                     PGDFAKKAFFLSTGAEAVENAVKIARSATKRSAVISFGGGFHGRTLLGMGLTGKVAPY
                     KAGFGPFPAEIYHAKFPNALHGVSVADSLASIEAIFKYDVEPERVAAIIIEPVQGEGG
                     FYACPPELAQALRALCDRHGIVFIADEVQTGAGRTGTWLACEQWGVAPDMVTMAKSMA
                     GGFPISAVIGRADLMDAPGPGGLGGTYAGSPIACAAALAVLDVFEEEKLLERSKALGA
                     RLVDGLKAIAAKHREVLEVRGLGAMVAIELFKDGDLAKPDADLTKRLCAEAAKRGLIL
                     LSCGSHANVVRVLVPLTAGDALVDEGLAILADAFAAVA"
     gene            4013..5404
                     /locus_tag="VF453_07375"
     CDS             4013..5404
                     /locus_tag="VF453_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7687509.1"
                     /translation="MFTLHPDAPTPLVTQIVDGLRRLVADGSLRPGAKIPSIRQFAKT
                     HRVSVFTVVEAYDRLVAQGYLVSRPHSGFFVRRRAAVEPAAVPGFASTASFDAQWYLR
                     SIFENRHLAVKAGCGWLPQSWLFEDGVRRSLRALAGEGVELDGYGDPRGHLPLREWIR
                     DAMAEHEVSLAPDQVLLTHGSSHALDLAIRRLVRAGDAVLVDDPGYSNLLFALRVAGA
                     RLIPVPRTPQGYDLAALEEAIATHHPRVFFTQPRLQSPTGSTATLPQLHRVLQLADRH
                     GLTIVENDLYVDLDPEPRPSLASLDQLARVIYVGSYSKTISPNLRVGYVAAHPALIDD
                     LAQLKMISGLTSSAFSERLVLGALTEGRWRKHLKGLRARLAQAHEATAQLLAGLGFEL
                     FAEPKAGMFLWARHAALADPVALSGRAAAHDVMLGPGNLFSATMQASDWMRFNVAFSS
                     DERLAAFLRQELA"
     gene            complement(5414..6274)
                     /locus_tag="VF453_07380"
     CDS             complement(5414..6274)
                     /locus_tag="VF453_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687510.1"
                     /translation="MSTSPEPADTSPAAPPPPWLSTQHEMEEAARPVRTRLARVRMRP
                     GVYRIAQATCLHAVVQSTTDARREHLYRVTLEWLAEFDTQTGHAEKIVDRATPGRTTN
                     TLQLRVLEQPGADGRMVALARLGPRGQIAAEPKGHGIMSFLRGVLVEWAAAEHPQAAV
                     VPDTFVHDPARGDNERQQREQFFARSGFVVTGDATGGRFGIGALRDLRTTWNTDKVLE
                     ITPALVAEAFGGQAQAGALKRQVEALESQIDGLARDRRAAEVMSRIWLAVTVLAVVFG
                     IVFGIQPRVA"
     gene            6498..9167
                     /gene="alaS"
                     /locus_tag="VF453_07385"
     CDS             6498..9167
                     /gene="alaS"
                     /locus_tag="VF453_07385"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX7687511.1"
                     /translation="MKAAEIRSTFLKFFEAKGHTIVPSSPVIPGDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCIRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFK
                     KDAISYAWELLTEYFKLPADKLWVTVYAEDDEAYDIWNKTVGVPAERIVRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNNVFMQFNRT
                     EDGVMHPLPKPSVDTGMGLERLAAVLQHVHSNYEIDLFVNLLAAAKKEVDAATPGNTP
                     ATVDVQAKLGGGCDKDSPSLKVIADHIRACSFTVADGVIPGNAGRGYVLRRIARRAIR
                     HGYKLGARTPFFHKLVAALAAEMGEAYPELRRDEARVTEVLKQEEERFFKTIATGMEL
                     LESALAGGAKQVDGETAFKLHDTFGFPVDLTADVCRERGVTVDEAGFHRLLEEQKERG
                     RQAGKFKMAQGLEYQGAATTFHGYEALVREDAKVVALYVDGSAVSSAHAGDDVVVVLD
                     HTPFYAESGGQVGDAGELRNATTRVVVDDTLKIQADVFGHHGQVAEGTIRVGDVFTAR
                     VDAERRARTVRNHSATHLMHKALREVLGAHVQQKGSLVNPERTRFDFAHTGPLTDEQI
                     RRIEAIVNAEVLANAGTDATVMAIDDAQKSGAMMLFGEKYGDEVRVLTIGSSKELCGG
                     THVERTGDIGLFKIVAESGVAAGIRRVEAVTGANALAYLQSLEHTVDEVAGALKATPE
                     EVPARLASVLDHVRALEKELEKLKGKLASAQGDELAGQAVDVKGLKVLAARLDGADAR
                     TLRETMDKLKDKLKTAAIVLAAVEGDKVQIAAGVTADSIGRVKAGELVNHVAQQVGGK
                     GGGKPDMAMVGGTQPANLAAALASVKGWVEQRA"
     gene            9315..9473
                     /locus_tag="VF453_07390"
     CDS             9315..9473
                     /locus_tag="VF453_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687512.1"
                     /translation="MPTTTVFLAAFATLAAALAAIAHARVVVATRGRMPRAWVVIRTD
                     RSRAPGRR"
     gene            9598..10212
                     /locus_tag="VF453_07395"
     CDS             9598..10212
                     /locus_tag="VF453_07395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7687513.1"
                     /translation="MSRHDAFPAASTHAAPAAARQDGVRLAVRRTRVSDAAAYARIMA
                     HPDVYPNLLQMPYPTPELWQAKLAESCAPGSPHVQVVVEAQVGDGAPEVVGSGGLHPA
                     GVSPRKRHLMMLGINVDAGWHGRGVGTLLMQSLCDYADHWLGLLRIELEVFADNHRAQ
                     ALYKRFGFVQEGYHRCDAMRGGAYVDSLSMARLNPKPLAGFPRG"
     gene            10311..11099
                     /locus_tag="VF453_07400"
     CDS             10311..11099
                     /locus_tag="VF453_07400"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEX7687514.1"
                     /translation="MNPLANVHYPTNTEADLDRLQGLCERLAGFDERVSLEWLDGAMT
                     ALAAGPRPRPLVEWRDRLLGDAWSRAVSDPEDERASTAILDARWRVLLRQLDPELLMD
                     APDELRLAPVMLEWTDEDKAALVADGTIEAGSEDEEIPLTGEVWTHGFLDVVELFKED
                     WTDPADPESDDALAYEECLARIALLGVHDPQELAQARDELFPEETLERDDLIQEALYA
                     CQDLRCYWVQNAPRPETRRVEPQPGRNDPCPCGSGKKFKKCHGA"
     gene            11131..12342
                     /gene="coaBC"
                     /locus_tag="VF453_07405"
     CDS             11131..12342
                     /gene="coaBC"
                     /locus_tag="VF453_07405"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX7687515.1"
                     /translation="MTDRLDLAGKHVVLGLSGGIACYKSAELARALVKAGATVQVVMT
                     AAAEEFITPVTMQALTGRPVFDSQWDARPGNNMAHINLSREADAILVAPASADFIAKL
                     VQGRADDLLSLLCLARPRERCPLLVAPAMNREMWSHPATQRNVAQLAADGAALLGPDV
                     GEQACLEFGEGRMLEAVDLRDELVAFFQPKPLCGRRVLVTAGPTFEAIDPVRGITNLS
                     SGKMGFAIARAAAEAGAEVTLVAGPVHLPTPRGVRRVDVQSARQMHEAVLPEAQAADV
                     FVATAAVADWRPAQMNEHKIKKDGKKVAPVFELAENADILADVARLPKPPYCVGFAAE
                     SENLLQHAREKLVRKKVPLIVGNLGPATFGRDDNALVLVDADGDRALPQADKLTLARQ
                     LVAEIARRLPA"
     gene            12417..12866
                     /gene="dut"
                     /locus_tag="VF453_07410"
     CDS             12417..12866
                     /gene="dut"
                     /locus_tag="VF453_07410"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX7687516.1"
                     /translation="MTVIDLKVLDARVAETLPAYATPGSAGLDLRACLDAPVVLAPGQ
                     TTLVPTGLAIHIGDAGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSVWNRG
                     QSEFTINPLERIAQMVIVPVVQATFRRVDDFDASHRGEGGFGSTGKH"
     gene            complement(12873..13481)
                     /locus_tag="VF453_07415"
     CDS             complement(12873..13481)
                     /locus_tag="VF453_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7687517.1"
                     /translation="MTARDRVGAGSADEDRRRALAGLARGTPWFLGALRAARALGLAA
                     WCIGAGAVRNLAWDALHGHATPSALADVDLAFFDATDLAAAREHALQARVQASCPSLR
                     WEVTNQAAVHTWFEARFGHAVAPLRSLEEAVASWPEFATAVGVTLAADDTIRVIAPHG
                     LDDLFAMVVRRNPARVSLATYRERIAAKRYPQRWPRVTVIEE"
     gene            complement(13492..14019)
                     /locus_tag="VF453_07420"
     CDS             complement(13492..14019)
                     /locus_tag="VF453_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7687518.1"
                     /translation="MNITAPCVVSLTWLLSDAQNALIDELSEPTEFFYGGDDLLPKVE
                     EALAGQEAGFDTHLHLEPENAFGDYDSDLVCFEDRALFPDGVEPGMQFEGLPDGAQTP
                     DMPEDAIYTVTEVYPSHVVLDGNHPLAGLALRMHITVRAVRPATPAEIDARSVNDDGM
                     EMLQVMPAVDRSKMH"
     gene            14074..>14254
                     /locus_tag="VF453_07425"
     CDS             14074..>14254
                     /locus_tag="VF453_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7687519.1"
                     /translation="MNLREPLALLGGLTPAAFMRDVWQKRPLLIRGALPPAGEDGAPL
                     APVTPAQLFALAARDD"
BASE COUNT         2003 a         5158 c         5134 g         1959 t
ORIGIN      
        1 gcggcaggca cgcgcgaggg ggcctcggcg gcggggcggt tggtcaggag cgcggccggc
       61 acgaccggcg tcgggccgcc gttgcggccg gtctgccagc cctggcccgc ccagtcgacg
      121 cgcaggtcct cgccgtcgga cgagtcgtcc agcgggccct tgttgacgag cctgggcggg
      181 acgtcgttga aggtccacag ctcctgcgcc ggcttctcgc cgcgcacggc cacggccacg
      241 acgaccaggc ccgccggatt ggcggccagg ctggtcgcgg tggaggcctc gttcgcgccc
      301 tcgcggcgcc acaggccgtg ggtgccgaag tcgaggtcga gcgcgccgtc gggccgcagg
      361 tggtacacga tcgcctgcgg cgcgatgccg ccggtcactt cgcccgcgac cagcaccgag
      421 ccgtcggcca gcggcagcag gtcgttgggc tgcaccggga cgccggcggg cgtcagctgc
      481 accttgccct ggacgcccca gcgcaggtcg ggggtgccgt cgggcaggaa gcgcgcgacc
      541 accggctgcg gctggttgcc cgctaggctc gccccgacca tccagatgcg atgggacgcg
      601 tccacgcgga cgctggcggg cgggtcgttg gtggtggcga cggtgaagtg cgcgaagccg
      661 tcgcgcccga agccggcgac gggctggccg gcgcggtcga ggtggcgcat caggaagtcg
      721 cgcgcatcgt cggccgggtc gtcctggatc gaccactggc tgccgtcggc gtcgcgcacc
      781 gtggtcgagc cggcatgcgc gggcccggcg gccagcagca tcgcggtcgc gagggccgcc
      841 gcgacgcggg ccgcgggcgg caaggcggcg cggctcattt ctggatgtcg ccgaggcaga
      901 ggtacttgac ctcgacgtag tcctcgatgc cctggcgcgc gccctcgcgg ccgaggccgg
      961 actgcttgac gccgccgaac ggcacctccg ccaccgagat catgccggtg ttgatgccca
     1021 ccatgccgga ctccagcgct tccgcgacgc ggaagatgcg cccgacgtcg cggctataga
     1081 aataggcggc gagcccgaac tccgtcgcgt tggcgagcgc gatcgcctcc gactcggtct
     1141 tgaagcgcac gaccggcgcg agcgggccga aggtctcctc cctggtcacg atcatctcgg
     1201 gcgtgacgtc ggccagcacc gtgggcgtga agaagcgctc gtcgatgcgg gcgccgccga
     1261 cgaccacgcg cgcgcccttg gctagcgcgt cctcgatgtg cgcctcgacc ttcgcgatcg
     1321 cgttcgcgtc gatcatcggg ccctggttga cgcccggctc gaagccgttg ccgaccttga
     1381 tgccgcgcgc cttctccgcc agccgcgcga tgaaggtgtc gtagacgccg tcctgcacgt
     1441 acagccggtt cgcgcagacg caggtctggc cggcgttgcg gtacttcgag atcatcgctc
     1501 cctcgaccgc ggagtcgatg tcggcgtcgt cgaacacgag gaagggggcg ttgccgccca
     1561 gctcgagcga gagcttcttg atcgtcggcg cgcactgctt catcaggatg cggccgacct
     1621 cggtggagcc ggtgaacgac aggtggcgca cgacctcgct cgcgcacagc tcgttgccga
     1681 cgaggatcga gttggccgca tcggccgtca cgacgttgag cacccccgcc ggcatgcccg
     1741 cgcgctgcgc gagctcggcc agcgcgagcg ccgacagcgg cgtctgctcc gccggcttga
     1801 tgaccaccgg gcagcccgcg gccagcgccg gcgcgacctt gcgggtgatc atcgcgatcg
     1861 ggaagttcca cggcgtgatc gccgcgcaca cgccgacggg ctgcttgagc acgaggtagc
     1921 gcttgccgtt gtcggtggtg gggatggtct cgccgtagac gcgcttggct tcctccgcga
     1981 accattcgat gaagctcgcg ccgtagacca cctcgccgcg cgcctcggcg atcggcttgc
     2041 cctgctcggc ggtgaggatg cgcgccaggt cgtcggcgtt ggcgacgatc agctggaacc
     2101 acttcatcat gaccgcgtgg cgctccttgg cggtcttggc gcgccacagc ggccaggcct
     2161 tgtcggcggc ggcgatggcg gccagcgcgt cggcgccggc gaggttggcg acgtcggcca
     2221 gcttctggcc ggtggccggg tcgacgacgt cgaagcgggc gccgcccgcg acccactcgc
     2281 cgccgacgag cgcgtcggtc ttgagcaggc ccgggtcgtt gagcgtggcg atgggggaag
     2341 tcttcatgtc cattcgaaac tcctgcagtc tgccgggcgg gacgcgttcc ggtgctcggc
     2401 gatggcgaca actctacccg ctgccgcccg ccgagcccgg cgtcgggaca ccgggttttc
     2461 cggcactttg gcggcccggg gcggcaacaa gatgaaacgc cgccggccgc ggccggagcg
     2521 gtcgcgggcg gcggcgccgg tgtgcgagcg gggcggtcag gcgacggcgg cgaacgcgtc
     2581 ggcgaggatg gccaggccct cgtcgaccag cgcgtcgccg gccgtcagcg gcaccagcac
     2641 gcgcaccacg ttggcgtggg agccgcagga cagcaggatc agcccgcgct tcgcggcctc
     2701 ggcgcacagg cgcttcgtga ggtccgcatc cggcttcgcg aggtcgccgt ccttgaagag
     2761 ctcgatggcc accatcgcgc ccaggccgcg cacctcgagc acctcgcggt gcttcgcggc
     2821 gatcgccttc aggccgtcga ccaggcgcgc gccgagcgcc ttcgagcgct cgagcagctt
     2881 ctcctcctcg aacacgtcca gcaccgcgag cgccgcggcg caggcgatcg gcgagccggc
     2941 gtaggtgccg cccaggccgc cggggccggg cgcatccatc aggtcggcgc ggccgatcac
     3001 ggccgagatc gggaagccgc ccgccatcga cttggccatg gtgaccatgt cgggcgcgac
     3061 gccccactgc tcgcacgcga gccaggtgcc ggtgcggccg gcgccggtct gcacctcgtc
     3121 ggcgatgaag acgatgccgt gcctgtcgca cagcgcgcgc agggcctgcg cgagctccgg
     3181 cgggcaggcg tagaagccgc cttcgccctg caccggctcg atgatgatcg cggcgacgcg
     3241 ctcgggctcg acgtcgtact tgaagatcgc ctcgatcgag gccagcgagt cggccacgct
     3301 cacgccgtgc agcgcgttcg ggaacttggc gtggtagatc tcggccggga acggaccgaa
     3361 gccggccttg tacggcgcga ccttgccggt caggcccatg cccagcagcg tgcggccgtg
     3421 gaagccgccg ccgaaggaga tcacggccga gcgcttcgtc gccgagcgcg cgatcttgac
     3481 cgcgttctcg acggcttccg cgccggtgga caggaagaac gccttcttgg cgaagtcgcc
     3541 cggcgtcttc gcgatcaggc gctcggccag ctcgatgtag ggctcgtagg cgagcacctg
     3601 gaagcaggtg tggtgcacct tgtcgagctg cgccttgacc gccgcgacga tcttcgggtg
     3661 gcggtgaccg gtgttgagca ccgcgatgcc gccggcgaag tcgatgtagc gcttgccctc
     3721 gacgtcccag acctcggcgt tctcggcgcg ctcgcacacg atgtcgtagg tctggccgag
     3781 gcccgagggc agggcggcgc ggcggcgcgc catcagggcg gcgttgttca ggtgggcgtc
     3841 tcgttgcatc gcggtctccg gcgccccttg gtggagcgat cagcaggtgg ggaatcgggc
     3901 cctcgtggag cccgggtcga gcgtgacttt agtgccgtga gcccggtaca gccatgtaca
     3961 gtcgacccga atcaccggtt cactgtgccg gtggcgatcc cggtacagat ccatgttcac
     4021 gctccacccc gatgccccga ccccgctcgt gacccagatc gtcgacggcc tgcgccgcct
     4081 cgtggccgac ggcagcctgc ggccgggcgc gaagatcccg tcgatccgcc agttcgcgaa
     4141 gacgcaccgc gtgagcgtgt tcaccgtcgt cgaggcctac gaccggctgg tcgcgcaggg
     4201 ctacctcgtc tcgcggccgc attccggctt cttcgtgcga cggcgggcgg cggtcgagcc
     4261 ggcggccgtg ccgggcttcg cctcgaccgc cagcttcgac gcccagtggt acctgcgcag
     4321 catcttcgag aaccgccacc tcgccgtgaa ggccggctgc ggctggttgc cgcagtcgtg
     4381 gctgttcgag gacggcgtgc ggcgatcgct gcgcgcgctg gcgggggagg gcgtcgagct
     4441 cgacggctat ggcgacccgc gcggccacct gccactgcgc gagtggatcc gcgacgcgat
     4501 ggccgagcac gaggtctcgc tggcgcccga ccaggtgctg ctcacccacg gctcgagcca
     4561 cgcgctggac ctcgcgatcc gccgcctcgt gcgcgcgggc gacgcggtgc tggtggacga
     4621 tcccggctac tcgaacctgc tgttcgcgct gcgcgtggcc ggcgcgcggc tgatcccggt
     4681 gccgcgcacg ccgcagggct acgacctcgc cgcgctcgag gaggcgatcg cgacgcacca
     4741 tccgcgcgtc ttcttcaccc agccgcggct gcagagcccc accggctcga ccgccacgct
     4801 gccccagctg caccgggtgc tgcagctggc cgaccgccac ggcctgacca tcgtcgagaa
     4861 cgacctctac gtcgacctcg accccgagcc gcggccctcg ctcgcgagcc tcgaccagct
     4921 ggcgcgcgtg atctacgtcg gcagctactc gaagacgatc tcgcccaacc tgcgcgttgg
     4981 ctacgtcgcg gcccatccgg cgctgatcga cgacctcgcg cagctgaaga tgatctcggg
     5041 cctgacctcg tcggccttca gcgagcgcct ggtgctgggc gcgctcacgg aagggcgctg
     5101 gcgcaagcac ctgaaggggc tgcgcgcccg gctcgcgcag gcgcacgagg cgacggcgca
     5161 gctgctggcg ggcctcggct tcgagctttt cgcggagccg aaggccggca tgttcctgtg
     5221 ggcgcgccat gccgcgctgg ccgacccggt cgcgctgtcc ggccgcgccg cggcgcacga
     5281 cgtcatgctg ggccccggca acctcttcag cgcgacgatg caggcgagcg actggatgcg
     5341 cttcaacgtc gcgttctcga gcgacgagcg gctcgccgcg ttcctgcgcc aggagctcgc
     5401 ctgaacgccc cgctcaggcg acccggggct ggatcccgaa cacgatgccg aacacgaccg
     5461 ccagcaccgt gaccgcgagc cagatgcgcg acatcacctc ggcggcgcgg cggtcgcgcg
     5521 cgaggccgtc gatctgcgac tcgagggcct cgacctggcg cttgagcgcg ccggcctgcg
     5581 cctggccgcc gaaggcctcg gccacgagcg ccggcgtgat ctcgagcacc ttgtcggtgt
     5641 tccaggtcgt gcgcaggtcg cgcagcgcgc cgatgccgaa gcggccgccg gtggcgtcgc
     5701 ccgtcacgac gaagccggag cgggcgaaga actgctcgcg ctgctggcgc tcgttgtcgc
     5761 cgcgtgccgg gtcgtgcacg aaggtgtcgg ggacgaccgc ggcctgcgga tgctccgccg
     5821 cggcccattc gaccagcacg ccgcgcagga agctcatgat gccgtggccc ttgggctcgg
     5881 ccgcgatctg gccgcgcggg ccgagccgcg cgagcgcgac catccggccg tcggcgccgg
     5941 gctgctccag cacgcgcagc tgcagcgtgt tggtcgtgcg gccgggcgtg gcgcggtcga
     6001 cgatcttctc ggcgtggccg gtctgggtgt cgaactcggc gagccactcg agcgtcacgc
     6061 ggtagaggtg ctcgcggcgt gcgtcggtcg tgctctggac gaccgcgtgc aggcaggtcg
     6121 cctgggcgat gcggtagacg ccgggccgca tgcgcacccg cgcgagccgc gtgcgcaccg
     6181 ggcgggcggc ttcctccatc tcgtgctgcg tgctcagcca gggcggcggc ggcgcggccg
     6241 gggacgtgtc ggcgggctcc ggggatgtgg acatggtcgt caggggcaat cgtcgcgcgc
     6301 attttggggc gggtggacgg gcggcgcgac ccgtgcgggc atcttccccg gccgagcgtg
     6361 aggtcgtgcg cacatcgccc gcggggcaag gcgagcgcca tgccgcctcc tataatcgcc
     6421 ggtccccgtc gcgccccgtg cgcgcgccac gcccgccggg gccgtcccgg ccggcgcgcc
     6481 cctcagaaag tgccggaatg aaagccgcag aaatccgttc gacgttcctg aagttcttcg
     6541 aggccaaggg tcacacgatc gtgccgtcgt cgccggtcat cccgggggac gatccgacgc
     6601 tgctgttcac caacgccggg atgaaccagt tcaaggacgt cttcctcggc ttcgacaagc
     6661 gcccgtactc gcgcgcgacg accgcgcaga aatgcatccg cgccggcggc aagcacaacg
     6721 acctcgacaa cgtcggctac acggcgcgcc accacacgtt cttcgagatg ctgggcaact
     6781 tcagcttcgg cgactacttc aagaaggacg cgatcagcta cgcctgggaa ctgctgaccg
     6841 agtacttcaa gctcccggcc gacaagctgt gggtcacggt ctacgccgag gacgacgagg
     6901 cgtacgacat ctggaacaag acggtgggcg tgcccgccga gcgcatcgtg cgcatcggcg
     6961 acaacaaggg cgcgcgctac gcctcggaca acttctggat gatgggcgac accggcccct
     7021 gcggcccgtg cacggagatc ttctacgacc acggccccga ggtcgcgggc ggcccgcccg
     7081 gctcgcccga cgaggacggc gaccgctaca tcgagatctg gaacaacgtc ttcatgcagt
     7141 tcaaccgcac ggaagacggc gtgatgcacc cgctgcccaa gccgagcgtg gacaccggca
     7201 tgggcctgga gcgcctggcc gccgtgctgc agcacgtgca ctcgaactac gagatcgacc
     7261 tgttcgtgaa cctgctggcg gcggcgaaga aggaagtcga cgccgccaca cccggcaaca
     7321 cgcccgcgac ggtcgatgtt caagcaaagc tcggtggcgg ttgcgacaag gactcgccct
     7381 cgctgaaggt catcgccgac cacattcgcg cctgctcgtt cacggtcgcc gacggcgtga
     7441 tcccgggcaa cgcgggccgc ggctacgtgc tgcgccgcat cgcgcgccgc gcgatccgcc
     7501 acggctacaa gctgggcgcg cgcacgccgt tcttccacaa gctcgtggcc gcgctggccg
     7561 ccgagatggg cgaggcctat cccgagctgc ggcgcgacga ggcgcgcgtc accgaggtcc
     7621 tgaagcagga agaagaacgc ttcttcaaga ccatcgcgac gggcatggag ctgctggagt
     7681 cggcgctggc cggcggcgcg aagcaggtcg acggcgagac cgcgttcaag ctgcacgaca
     7741 ccttcggctt ccccgtcgac ctgacggccg acgtctgccg cgagcgcggc gtgaccgtcg
     7801 acgaggcggg cttccaccgc ctgctcgagg agcagaagga acgcggccgc caggccggca
     7861 agttcaagat ggcgcagggc ctcgagtacc agggcgcggc gaccaccttc cacggctacg
     7921 aggcgctcgt gcgcgaggac gcgaaggtcg tcgcgctgta cgtcgacggc agcgccgtgt
     7981 ccagcgccca cgcgggcgac gacgtcgtgg tcgtcctcga ccacacgccg ttctatgccg
     8041 agagcggggg ccaggtcggc gacgccggcg agctgcgcaa cgcgacgacg cgcgtggtcg
     8101 tcgacgacac gctcaagatc caggccgacg tgttcggcca tcacgggcag gtcgccgagg
     8161 gcacgatccg ggtcggcgac gtgttcaccg cccgcgtcga cgccgagcgc cgcgcccgca
     8221 ccgtgcgcaa ccacagcgcg acccacctga tgcacaaggc gctgcgcgag gtgctgggcg
     8281 cgcacgtgca gcagaagggc tcgctcgtga acccggagcg cacgcgcttc gacttcgcgc
     8341 acaccggccc gctgacggac gagcagatcc gccggatcga ggccatcgtc aacgccgagg
     8401 tgctggccaa cgccggcacc gacgcgacgg tcatggcgat cgacgatgcg cagaagtcgg
     8461 gcgcgatgat gctgttcggc gagaagtacg gcgacgaggt gcgcgtgttg accatcggtt
     8521 cgagcaagga gctgtgcggc ggcacgcacg tcgagcgcac gggcgacatc ggcctgttca
     8581 agatcgtggc cgagtcgggc gtcgcggccg gcatccgtcg cgtggaggcg gtcaccggcg
     8641 ccaatgcgct ggcctacctg cagtcgctgg agcacaccgt cgacgaggtc gccggcgcgc
     8701 tcaaggcgac gcccgaagaa gtgccggcgc ggctcgcgtc ggtgctggac cacgtccgcg
     8761 cgctggagaa ggagctcgag aagctcaagg gcaagctcgc gtccgcgcag ggcgacgagc
     8821 tggccgggca ggcggtcgac gtcaagggcc tgaaggtgct cgccgcgcgc ctggacggcg
     8881 ccgacgccag gacgctgcgc gagaccatgg acaagctgaa ggacaagctc aagaccgccg
     8941 ccatcgtcct ggccgcggtc gagggcgaca aggtgcagat cgccgccggc gtgaccgccg
     9001 actcgatcgg ccgcgtgaag gcgggcgagc tggtcaacca cgtcgcccag caggtcggcg
     9061 gcaagggcgg cggcaagccc gacatggcga tggtcggcgg cacgcagccc gccaacctcg
     9121 cggccgcgct cgcgagcgtg aagggctggg tcgagcagcg cgcctgacgc cgcccggccg
     9181 ccgcccggcc gccgcccgcc gacgcccgcc tcgtgcgggc gctttccttt ccggccttcg
     9241 ttcgtgcgtc cgtcgcgtcg attcgtcgac ggcgccttga cgctcccgcg gccagccctc
     9301 agactgcacc gcacatgccc acgacgaccg tgttcctcgc cgccttcgcc acgctagccg
     9361 ctgcgctcgc cgcgatcgcc catgcgcgtg tcgtcgtcgc gacccggggc cgcatgccgc
     9421 gcgcctgggt cgtcattcgc accgaccgga gtcgggcccc gggccggcgc tgactccgaa
     9481 tccccgccag atcccccgca caaggcccgc cacccgcggg ccttgcgctt tcttgcgctc
     9541 cggttcgccg cagcgtccac gccgccgacc caggcaccgt cccaccagga gatgttcatg
     9601 agccgacacg acgccttccc cgccgccagc acccacgccg cccccgccgc cgcccgccag
     9661 gacggcgtgc gcctggcggt gcgccgcacg cgcgtgtcgg acgcggcggc gtacgcgcgg
     9721 atcatggcgc atccggacgt ctacccgaac ctgctgcaga tgccgtaccc gacgcccgag
     9781 ctctggcagg cgaagctggc cgagagctgc gcgccgggca gcccgcatgt ccaggtcgtc
     9841 gtcgaggcgc aggtcggcga cggcgcgccg gaggtcgtcg gctcgggcgg cctgcatccg
     9901 gccggcgtgt cgccgaggaa gcgccacctg atgatgctgg gcatcaacgt cgatgccggc
     9961 tggcacggcc gcggcgtcgg cacgctgctg atgcaatcgc tgtgcgacta cgccgaccac
    10021 tggctcgggc tgctgcgcat cgagctcgag gtcttcgcgg acaaccaccg cgcacaggcg
    10081 ctctacaagc gcttcggctt cgtgcaggag ggctaccacc gctgcgacgc gatgcgcggc
    10141 ggcgcctacg tcgactcgct gtcgatggcg cggctcaacc cgaagccgct cgccggcttc
    10201 ccgcgcggct gagaacgccc ccgcgcaggc gtcccgcgcg agcttgcgca cgcgcccgcg
    10261 gcggccggca tgagacactg ccggccgcat ttcgctttcg cacgtccatc atgaacccgc
    10321 tcgccaacgt ccactacccg accaacaccg aagccgacct cgaccgcctg cagggcctgt
    10381 gcgagcggct cgcgggcttc gacgagcgcg tctcgctcga gtggctggac ggcgcaatga
    10441 ccgcgctggc cgccggcccg cgaccgcggc cgctcgtcga gtggcgcgac cgcctgctcg
    10501 gcgacgcctg gtcgcgcgcg gtgtccgacc ccgaggacga gcgcgccagc accgccatcc
    10561 tcgacgcccg ctggcgcgtg ctgctgcgcc agctcgaccc cgagctgctg atggacgcgc
    10621 ccgacgagct gcgcctcgcg cccgtgatgc tcgagtggac ggacgaggac aaggccgcgc
    10681 tcgtcgccga cggcacgatc gaggcgggct cggaggacga ggagatcccg ctgacgggcg
    10741 aggtctggac gcacggcttc ctcgatgtcg tcgagctgtt caaggaggac tggacggatc
    10801 ccgccgatcc cgagagcgac gacgcgctcg cctacgagga atgcctcgcg cgcatcgcgc
    10861 tgctgggcgt gcacgatccg caagagctcg cgcaggcgcg cgacgagctg ttccccgagg
    10921 agacgctgga gcgcgacgac ctgatccagg aagcgctcta cgcctgccag gacctgcgct
    10981 gctactgggt gcagaacgcg ccgaggcccg agacgcgccg cgtcgagccg cagccgggcc
    11041 gcaacgatcc gtgcccgtgc ggcagcggca agaagttcaa gaagtgccac ggcgcgtgac
    11101 gcctcccggg cgcgcgtacc attgcgcccc atgaccgatc gactcgatct cgcgggcaag
    11161 cacgtcgtgc tgggcctgtc cggcggcatc gcctgctaca agtccgcgga gctcgcgcgc
    11221 gcgctcgtga aggccggcgc gaccgtgcag gtcgtgatga cggccgccgc cgaggaattc
    11281 atcacgccgg tgacgatgca ggcgctcacg gggcgccccg tgttcgacag ccagtgggac
    11341 gcgcgccccg gcaacaacat ggcgcacatc aacctctcgc gcgaggccga tgccatcctc
    11401 gtcgcgccgg cgagcgcgga tttcatcgcg aagctggtgc agggccgcgc ggacgacctg
    11461 ctgtcgctgc tgtgcctcgc ccgcccgcgc gagcgctgcc cgctgctcgt cgcgccggcg
    11521 atgaaccgcg agatgtggtc gcacccggcg acgcagcgca acgtcgccca gctcgcggcg
    11581 gacggtgcgg cgctgctcgg cccggacgtc ggcgagcagg cctgcctgga gttcggcgag
    11641 ggccgcatgc tcgaggcggt cgacctgcgc gacgagctgg tcgcgttctt ccagcccaag
    11701 ccgttgtgcg gcaggcgcgt gctcgtcacc gccggcccca ccttcgaggc gatcgacccg
    11761 gtgcgcggca tcaccaacct ctcgagcggc aagatgggct tcgccatcgc gcgcgcggcc
    11821 gccgaggcgg gcgccgaggt gaccctggtc gccggcccgg tgcacctgcc cacgccgcgc
    11881 ggcgtgcggc gcgtcgacgt ccagagcgcg cgccagatgc acgaggccgt cctgcccgag
    11941 gcgcaggcgg ccgacgtctt cgtcgccacg gcggcggtcg ccgactggcg gcccgcgcag
    12001 atgaacgagc acaagatcaa gaaggacggc aagaaggtcg cgcccgtctt cgagctcgcc
    12061 gagaacgcgg acatcctcgc cgacgtcgcg cggctgccga aacccccgta ctgcgtcggc
    12121 ttcgcggccg agagcgagaa cctgctgcag cacgcgcgcg agaagctcgt gcgcaagaag
    12181 gtgccgctga tcgtcggcaa cctcggcccg gcgaccttcg gccgcgacga caatgcgctg
    12241 gtgctcgtcg acgccgacgg cgaccgcgcg ctgccgcagg ccgacaagct cacgctcgcg
    12301 cgccagctcg tggccgagat cgcccgccgc ctgccggctt gagcgatcgg ccgccggcgc
    12361 gccgatccgc acgcccatcg attccgcttc cgcgtcgacc ccgacccgcc actgccatga
    12421 ccgtcatcga cctcaaggtg ctcgacgccc gcgtcgccga gaccctgccc gcctacgcga
    12481 cccccggcag cgccggcctg gacctgcgcg cctgcctgga cgccccggtc gtcctcgcgc
    12541 ccggccagac cacgctcgtg cccaccggcc tcgcgatcca catcggcgac gccggcctgg
    12601 ccgcgatgat cctgccgcgc tcgggcctgg gccacaagca cggcatcgtg ctgggcaacc
    12661 tcgtcgggct gatcgactcc gactaccagg gccagctcat ggtcagcgtg tggaaccgcg
    12721 gccaatcgga gttcacgatc aacccgctgg agcgcatcgc gcagatggtc atcgtgccgg
    12781 tggtgcaggc cacgttccgc cgcgtcgacg acttcgacgc ctcgcatcgt ggcgagggtg
    12841 ggttcggttc cacgggcaag cactgacgcg cgtcattcct cgatcacggt gacccgcggc
    12901 cagcgctgcg gatagcgttt cgccgcgatg cgctcgcggt aggtcgcgag gctgacccgc
    12961 gccggattgc ggcgcaccac catcgcgaag aggtcgtcga gcccgtgcgg cgcgatcacg
    13021 cggatcgtgt cgtcggcggc gagcgtgacg ccgacggcgg tcgcgaactc gggccatgac
    13081 gcgacggcct cctcgagcga gcgcagcggc gccacggcgt ggccgaagcg cgcctcgaac
    13141 cacgtgtgga cggcggcctg gttggtgacc tcccagcgaa gcgacgggca ggacgcctgc
    13201 acgcgcgcct gcaaggcgtg ctcgcgggcg gcagccaggt ccgtcgcgtc gaagaaggcg
    13261 aggtcgacgt cggcgagcgc ggagggcgtc gcgtggccgt gcaaggcgtc ccaggccaga
    13321 ttgcgcaccg cgccggcgcc gatgcaccac gcggccaggc cgagcgcccg ggcggcgcgc
    13381 agcgcgccga ggaaccaggg cgtgccccgc gcgaggccgg cgagcgcgcg gcgccggtct
    13441 tcgtcggcgg agccggcacc gacccgatcc cgcgccgtca tcgcgccgcc gtcagtgcat
    13501 cttcgagcgg tcgaccgccg gcatcacctg cagcatctcc atgccgtcgt cgttgacgct
    13561 gcgtgcgtcg atctcggccg gcgtggccgg gcgcacggcg cggacggtga tgtgcatgcg
    13621 cagcgcaagg ccggcgagcg gatggttgcc atcgagcacg acgtgcgagg ggtagacctc
    13681 ggtgaccgtg tagatcgcgt cctccggcat gtcgggcgtc tgcgcgccgt cgggcagccc
    13741 ttcgaactgc atgccgggct cgacgccgtc ggggaacagc gcgcgatcct cgaagcagac
    13801 gaggtcggag tcgtagtcgc cgaacgcgtt ctcgggttcg aggtgcaggt gggtgtcgaa
    13861 gcccgcctcc tggcccgcga gcgcttcctc gaccttgggc agcaggtcgt cgccgccgta
    13921 gaagaactcg gtcggctcgg acagctcgtc gatcagcgcg ttctgggcgt ccgacagcag
    13981 ccaggtgagc gacacgacgc agggggcggt gatgttcatc gcgcgattct acgagcgggt
    14041 ggaccgcggc gcgagtcgac ggacaatgcc gggatgaacc tccgagaacc cctcgcgctg
    14101 ctgggcggcc tcacgcccgc ggccttcatg cgcgacgtct ggcagaagcg gccgctgctg
    14161 atccgcggcg cattgccgcc agcgggcgag gacggtgcgc cgctcgcgcc cgtcacgccg
    14221 gcgcagttgt tcgcgctcgc ggcccgcgac gacg
//
LOCUS       DASVLN010000302         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_446138, whole genome shotgun sequence.
ACCESSION   DASVLN010000302 DASVLN010000000
VERSION     DASVLN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446138"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF453_07430"
     CDS             complement(<1..345)
                     /locus_tag="VF453_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687520.1"
                     /translation="MSRTSPFVEPVAAPAAPTIPANHARYAAMDRALQRTPGAIPCHW
                     FAAAACVTHPRRGLGVLDHWCGLLVASAEEARFLRHVHHGLARMNASWFERLVSGGEI
                     PTCEGLRGAALDH"
     gene            complement(913..2211)
                     /locus_tag="VF453_07435"
     CDS             complement(913..2211)
                     /locus_tag="VF453_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687521.1"
                     /translation="MTICKGVGKSPSDQKTLNEELIEALVEVAQRNPQHAVELFGVPL
                     GVAMRLAAMPDAQLDDLATTPVALWRPALTERLVRLLPEWRPMPAPDLEAYRPLVKRI
                     NRLALATFVRYADDPVSAALVCNLSDVGATIALQELPAFAFLEWAGNPGTLLIAPRLT
                     DALIDRLLRPDDDTGVEPHLRGLFALVQCCDQEVAAIRHALHQESLAWRTSEQPAALA
                     SKRCGRPPASFLMPDTSSLILTMLRHQMRPAEVEDRLLPTGAVRAAQLRVVAETVCPR
                     PKRAKNTPRDRTRDNRDLWGSAYRRLSATAIFRLQRRLVDAGQPAFEAMVHAYDYYAL
                     NYDADCGVSLSRMLKDVFSSMREGTDTRVAHCARCGILHLSHEEKSGIIECPVCVLAR
                     AGKFGQRRVRLDRVESALLPEEHRVWSGGAAWEEGLAAAP"
     gene            2456..>2776
                     /locus_tag="VF453_07440"
     CDS             2456..>2776
                     /locus_tag="VF453_07440"
                     /inference="COORDINATES: protein motif:HMM:NF020251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7687522.1"
                     /translation="MDRPAPSPRPALDAIRGDEPPASAGVRRFLLAHGVKEHNHASEI
                     ARIIAVDRSQSYRRLKGDVAWSHTDLRAIARHFGAPEGSLLPGDEATRGGSVAPAASA
                     QPAAA"
BASE COUNT          390 a          981 c         1019 g          386 t
ORIGIN      
        1 gtggtccagc gcggcgccgc gcagtccctc gcaggtcggg atctcgcccc cggagaccag
       61 ccgctcgaac caggacgcgt tcatccgggc gagcccatga tgcacgtggc gcaggaagcg
      121 cgcctcctcg gcgctggcga cgagcaggcc gcaccagtgg tcgaggacgc cgaggccgcg
      181 gcgcggatgc gtgacgcagg ccgcggcagc gaaccagtgg cacgggatcg cgccgggcgt
      241 ccgctgcagc gcgcgatcca tcgcggcgta gcgggcgtgg ttggccggga tggtcggcgc
      301 ggcgggcgca gccacaggct cgacgaacgg cgacgtgcgc gacatgggca tgccgctccc
      361 gagcgagccg catgcccgga tgggctgggc gcgagcggcg agccggcccc cgccggggca
      421 tgcgaccggg cgacatccgc ttgggccgct ggcgccgcac catcgggcgc ggagtgcgcg
      481 gtgcagcgtg cggagcgaca agcgccggat gccggatgcc ggatgccgga tgccggatgc
      541 cggttgccgg ttgccggttg ccggttgccg gttgcccaat gccgaacgcc gaacgccgaa
      601 cgccgagttt cgagtgccga gcacccggag tcgagcatcg accttcgatg cagctgttgt
      661 cacgggtcga gcgccaagcc agcgaccgcg accgcgccgg aacgcgagcg cccgatcgcc
      721 gacgtcggac ggccgctcgc cgggcgtggc accggtggcc acgcccggcc ggttcgaccc
      781 gggagcgcgc agggcgcctc ccccgccacg agcgctgcat ccccggtctt gctcgggaaa
      841 gagaccacgc ccggccagtg cgcgcacgca cacccggccg gacgagtcgc gatccgtgcg
      901 ctgtggcgct cgctagggcg cggcggccag gccctcctcc cacgcggcgc cgccgctcca
      961 gacgcggtgc tcctcgggca gcagcgcgga ctcgacgcgg tcgagccgca cacggcgctg
     1021 gccgaacttg cccgcgcgcg ccaggacgca gaccgggcat tcgatgatgc cgctcttctc
     1081 ttcgtgcgag aggtgcagga tcccgcagcg cgcgcagtgg gcgacgcgcg tgtccgtgcc
     1141 ctcgcgcatc gacgagaaga cgtccttgag catgcggctc agggagacgc cgcagtcggc
     1201 gtcgtagttg agcgcgtagt agtcgtaggc atgcaccatc gcctcgaacg cgggctggcc
     1261 ggcgtccacg aggcggcgct gcagccggaa gatcgccgtc gcgctgagcc ggcggtacgc
     1321 gctgccccac aggtcgcggt tgtcgcgcgt gcgatcgcgc ggcgtgttct tcgcgcgctt
     1381 cgggcgcggg cagacggtct cggcaacgac ccgcagctgc gcggcgcgca cggcgccggt
     1441 cggcagcagg cgatcctcga cctcggccgg ccgcatctgg tggcgcagca tcgtcaggat
     1501 cagcgacgag gtgtccggca tcaggaagct cgccggcggc cggccgcagc gcttgctcgc
     1561 gagggcggcg ggctgctccg aggttcgcca cgcgagcgat tcctggtgca gcgcatggcg
     1621 aatcgcggcg acctcctggt cgcagcactg cacaagcgcg aacaggccgc gcaggtgggg
     1681 ttcgacgccc gtgtcgtcat cgggacgcag caggcggtcg atcagggcgt cggtcaggcg
     1741 cggcgcgatg agcagcgtgc ccgggttgcc ggcccattcc aggaatgcga aggccgggag
     1801 ctcctgcaac gcgatcgtgg cgccgacatc ggacaggttg cacacgagcg cggcgctgac
     1861 cgggtcgtcg gcatagcgca cgaaagtcgc gagcgccagg cggttgatgc gcttgaccag
     1921 cggccggtag gcctccaggt cgggcgccgg catcgggcgc cattcgggca ggagacggac
     1981 gaggcgctcg gtcagcgcgg gccgccacag tgcgacgggc gtcgtcgcca ggtcgtcgag
     2041 ctgggcgtcg ggcatggcgg cgagccgcat cgccaccccc agcgggacgc cgaacaattc
     2101 caccgcgtgc tgcggatttc gctgggccac ctcgaccagg gcctctatca gttcctcgtt
     2161 gagcgtcttc tggtcgctcg ggctctttcc gacccctttg cagatcgtca tcgatagtcc
     2221 tccatcaaac ggttcccgcg cgggaagcac cgcctcaaaa gccttttctg tggactgccg
     2281 ccacattcga tcttcgttgt ccttttgtgg gtgcacgcga ctttgacaca caacttattc
     2341 cccatcaatg tgcatttgtg ggctttttgt tgtgaatcag gggctcaact ttggggggtc
     2401 gaggcggcgg cgcccgcaca atgacgccgt ccatcgtccg aaacagccgc ctgccatgga
     2461 tcgtcccgcc ccgtcgccgc gccccgccct cgacgccatc cgcggcgacg agccgcccgc
     2521 cagcgccggc gtgcgccgct tcctgctagc gcacggggtg aaggagcaca accatgcgtc
     2581 ggagatcgcg cgcatcatcg ccgtcgaccg ctcgcagtcg taccggcgcc tgaagggcga
     2641 tgtggcttgg agccataccg acctgcgcgc gatcgcccgc cacttcggcg cgccggaggg
     2701 ctcgctgctg cccggcgacg aggccacgcg cggcggcagc gtcgcgccgg cggcgtccgc
     2761 acagccggcg gcggcg
//
LOCUS       DASVLN010000303         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_68989, whole genome shotgun sequence.
ACCESSION   DASVLN010000303 DASVLN010000000
VERSION     DASVLN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68989"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1365)
                     /locus_tag="VF453_07445"
     CDS             complement(82..1365)
                     /locus_tag="VF453_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7687523.1"
                     /translation="MKKFILAAAAAAAAMLAGGAGAAQGYVPGGVPALDHVFVIMMEN
                     HAYQQVAGNPQAPFINALMKHANVATNYYAIAHPSSTNYLEVVGGSNFNNLSDQYPDW
                     HDVSCVPNIAPGRPTNLDNPSTGTVCPIRGGHGTDAATPAFDLSQNETSAPPLTNIDG
                     KHAIPAGHDIDGISIADQLVRAGKSWKSYQEGLPGIGADGVNVSDGYYSIGGRNALGE
                     SVTSDFAALSTAGNPVSASDVVYLYAVKHNPFAYFASVQEGRDPALSLRRVRAFDGEH
                     GLYADLKAGSVPNFAFIAPNQCDDMHGRGNGTAFCNYDADDNGTQAGLNPSLILQGDQ
                     SVQRIVTAIKASRVWAERRSAIVILWDENDYSTSTSNQVVAIVDTNYGARRVTSAKYY
                     NHYSLTKTLDAAFGLPCLNHACDAGVEVMSDLFRP"
     gene            1580..1933
                     /locus_tag="VF453_07450"
     CDS             1580..1933
                     /locus_tag="VF453_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7687524.1"
                     /translation="MNRTDLIELLAERHELSKAEAGRVLGTLLDAIVTTVKKGGAVTL
                     PGFGSFKQSSRSARTGRNPSTGEAVKIAATKVPRFTAGAGFKDAVDPKAAARRAAKKG
                     AAAPAKKAPAKKKGR"
     gene            complement(1971..3131)
                     /locus_tag="VF453_07455"
     CDS             complement(1971..3131)
                     /locus_tag="VF453_07455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX7687525.1"
                     /translation="MPVITHIEDLRVLAERRVPRMFYDYADSGSWTETTYRRNESDFA
                     RIQLRQRVAVDMSDRTTAVTMAGQAARMPVAIAPVGLTGMQHADGEIHAARAAEKFGI
                     PFTLSTMSICSIEDIAEHTQAPFWFQLYMMRDREAMKRMIGRAKAARCSALVLTLDLQ
                     VIGQRHKDLKNGLTAPPRPTLRNLANLATKPRWCLGMLGTRRHTFRNLVGHVQGVSDV
                     RSLAAWTNEQFDPRLAWPDVAWVKEQWGGKLILKGIMDAQDARLAVDAGADAIVVSNH
                     GGRQLDGAPSSIAALPAIVDAVGGRCEVWMDGGIRSGQDVLKAWALGARGTMIGRAMA
                     YGLGAMGEAGVTRALEILHKELDVTMAFCGHTDIADVGRHILVPGTEPRLGC"
BASE COUNT          450 a         1183 c         1209 g          509 t
ORIGIN      
        1 gccgcgagga tcgatcgaag gctcatggac ggccgcctgc gggcctgcgc gtcccggccc
       61 gcgcgcgcgg gcggcggccc gtcacgggcg gaacaggtcc gacatcacct ccacgccggc
      121 gtcgcaggcg tggttcaggc agggcaggcc gaacgcggcg tcgagcgtct tcgtcagcga
      181 gtagtggttg tagtacttcg cgctggtcac gcggcgcgcg ccatagttcg tgtcgacgat
      241 ggccacgacc tggttcgagg tgctcgtgct gtagtcgttc tcgtcccaca ggatgacgat
      301 cgcgctgcgg cgctccgccc agacgcgcga ggccttgatc gcggtgacga tgcgctgcac
      361 cgactggtcg ccctgcagga tcagcgacgg gttgaggccg gcctgcgtgc cgttgtcgtc
      421 ggcgtcgtag ttgcagaacg ccgtgccgtt gccgcggccg tgcatgtcgt cgcactggtt
      481 cggcgcgatg aaggcgaagt tgggtaccga gcccgccttc aggtcggcgt acaggccgtg
      541 ctcgccgtcg aaggcccgga cgcgcctcag gctcagcgcc gggtcgcggc cttcctgcac
      601 cgaggcgaag tacgcgaacg gattgtgctt gaccgcgtac aggtacacga cgtccgaggc
      661 cgacaccggg ttgcctgcgg tcgacagcgc cgcgaagtcc gaggtcacgg actcgccgag
      721 cgcgttcctg ccgccgatgc tgtagtagcc gtcgctgacg ttgacgccgt cggcgccgat
      781 gccgggcagg ccttcctggt agctcttcca ggacttgccg gcgcgcacga gctggtcggc
      841 gatggagatg ccgtcgatgt cgtggccggc cgggatcgcg tgcttgccgt cgatgttggt
      901 cagcggcggc gcggaggtct cgttctggga caggtcgaac gcgggcgtgg ccgcgtccgt
      961 gccgtggccg ccgcggatcg ggcagacggt gccggtgctc gggttgtcga ggttggtcgg
     1021 gcggccgggg gcgatgttcg ggacgcagga gacgtcatgc cagtcggggt actggtccga
     1081 caggttgttg aagttcgagc cgccgacgac ctcgaggtag ttcgtcgagc tcggatgcgc
     1141 gatcgcgtag tagttggtcg cgacgttggc gtgcttcatg agcgcgttga tgaagggcgc
     1201 ctgcgggttg cccgcgacct gctggtaggc gtggttctcc atcatgatca cgaacacgtg
     1261 gtcgagcgcg ggaacgccgc cgggcacata gccctgggcc gcgccggcgc cgccggcaag
     1321 catggctgcc gccgcggcgg ccgcggcgag gatgaacttc ttcatggctg tgtcgctcct
     1381 ggcttttatg gatatggcgg ccgtccatcg tcgcgaggac ggtccggccg gccatgctgc
     1441 gcgccgcaca tgaccggcgc gtgtcgccgc gccagggcag ggaagacccg gacccgcggt
     1501 gacgtctcgt cacgggcggc gccgcatctg ctgcgacaat cgcgccgccc gcgctgttgc
     1561 gggcgcagtc accatccgca tgaatcgcac tgacctcatc gagctgctgg ccgagcgcca
     1621 cgagctctcc aaggccgaag ccggccgcgt cctgggcacg ctcctcgacg ccatcgtcac
     1681 caccgtcaag aagggcggcg ccgtcacgct gccgggcttc ggcagcttca agcagtcctc
     1741 gcgcagcgcg cgcacgggcc gcaacccgtc gaccggcgaa gccgtgaaga tcgccgcgac
     1801 caaggtgccg cgcttcaccg ccggcgccgg cttcaaggac gccgtcgacc cgaaggccgc
     1861 cgcgcgccgc gccgccaaga agggcgccgc cgcgcccgcc aagaaggcgc ccgccaagaa
     1921 gaagggccgc tgatcacgcc ctgggggccg cccaccggcg gctcccgcct tcagcagccc
     1981 agccgcggct ccgtgcccgg cacgaggatg tgccggccga catcggcgat gtccgtgtgg
     2041 ccgcagaagg ccatcgtcac gtcgagctcc ttgtgcagga tctcgagcgc gcgcgtcacg
     2101 cccgcctcgc ccatcgcgcc cagcccgtag gccatcgcgc gcccgatcat cgtgccgcgc
     2161 gcgcccagcg cccaggcctt gagcacgtcc tggcccgagc ggatcccgcc gtccatccag
     2221 acctcgcagc gcccgcccac cgcgtccacg atcgccggca gcgcggcgat gctcgagggc
     2281 gcgccgtcca gctggcggcc gccgtggttg ctgacgacga tcgcgtccgc gccggcgtcg
     2341 acggccagcc gcgcgtcctg cgcgtccatg atccccttga ggatcagctt gccgccccat
     2401 tgctccttga cccacgcgac gtccggccac gcgagccgcg gatcgaactg ctcgttcgtc
     2461 caggccgcga gcgagcgcac gtcgctcacg ccctgcacgt ggccgaccag gttgcggaag
     2521 gtgtgccgcc gcgtgcccag catgcccagg caccagcgcg gcttggtcgc gaggttggcg
     2581 aggttgcgca gcgtcggccg cggcggcgcg gtcaggccgt tcttcaggtc cttgtggcgc
     2641 tggccgatca cctgcaggtc cagcgtgagc accagcgcgc tgcagcgcgc ggccttcgcg
     2701 cgcccgatca tccgcttcat cgcctcgcgg tcgcgcatca tgtagagctg gaaccagaac
     2761 ggcgcctgcg tgtgctcggc gatgtcctcg atcgagcaga tgctcatcgt cgacagcgtg
     2821 aacgggatgc cgaacttctc ggccgcgcgc gcggcgtgga tctcgccgtc ggcgtgctgc
     2881 atgcccgtca gcccgacggg cgcgatggcc accggcatcc gcgcggcctg gccggccatc
     2941 gtcacggcgg tcgtgcggtc cgacatgtcg accgccacgc gctggcgcag ctggatgcgc
     3001 gcgaagtcgc tctcgttgcg gcggtaggtc gtctccgtcc aggagccgga gtcggcgtag
     3061 tcgtagaaca tccggggcac gcgcctctcg gcgagcacgc gcaggtcttc gatgtgggtg
     3121 atcacgggca tgcgcccatg ctaggacggc cgcgccgccc gcggccggaa agattttcag
     3181 cgcacgccgg aaacgaacgc ctgcgactcg tcggcgatgc gggtgcagca ctgcgccagc
     3241 caggcggcga aggcctcggc ggcgcggccg ctcgcatgcg gcagcaggtg gtagctcgcg
     3301 gccggccgca ccgcgagcgc gaacggcgcg acgagccgcc cctcgcgcag c
//
LOCUS       DASVLN010000304         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_284824, whole genome shotgun sequence.
ACCESSION   DASVLN010000304 DASVLN010000000
VERSION     DASVLN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284824"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..385)
                     /locus_tag="VF453_07460"
     CDS             complement(86..385)
                     /locus_tag="VF453_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687526.1"
                     /translation="MFILDGSRQVFGPGVIRDFREFVGVEATVTTPITSYFDLQGAVG
                     PGRTHMDGGDPDTTRYVTEGVVRAARLPSTITAVGPAIASTRTASSNARAGRSGF"
     gene            complement(510..>1685)
                     /locus_tag="VF453_07465"
     CDS             complement(510..>1685)
                     /locus_tag="VF453_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX7687527.1"
                     /translation="APAASAAPAAIATVPGMPPVVDPRNLYSETDGPAKISPALQGDL
                     VRVYVPNLRGNSVSVIDPAKMKVVDTFKVGQSPQHVVVGYDLRTLWAANNAERSSNGS
                     LTPIDPRTGKPGTAIPVDDPYNMYFTPDGKSAIVVAEARHRLDFRDPKSMALQYSIDA
                     PHCGGINHADFSIDGRTAFFTCEFDGSIARIDLVNRKVDGYLVLKMPATRLKDHAVPE
                     GAPTTEICTSKKGMPQDIRFSPDGRTLYVADMEADGVHLLDAASFTETGFIPTGVAAH
                     GLYPSRDGTKLYIANRGSHRIHGPRHGPGSVSVLDFATNKIVANWKIPGGGSPDMGNV
                     TADGKYLWLSGRYDDVVYRFDTANGDVREIPVGQEPHGLTVWPQPGRYSLGHTGNLR"
BASE COUNT          223 a          558 c          638 g          266 t
ORIGIN      
        1 atcgccgcgc ccaggccccg cccctgcgac gccggggcga gctgcacctg ctccagctgc
       61 cagggatcgc cctcgcgcac gggcttcaga agcccgatcg cccggccctc gcgttcgacg
      121 atgcagtccg cgtcgaggcg atggccgggc cgacggcggt gatcgtcgag ggcaatcggg
      181 cagccctgac gacgccctcg gtgacgtagc gcgtggtgtc gggatcgccg ccgtccatgt
      241 gggtgcggcc agggcccacg gctccctgga ggtcgaagta cgaggtgatc ggcgtcgtca
      301 ccgtcgcctc gacgcccacg aattcgcgga aatcgcggat gacgccggga ccgaacacct
      361 ggcgggagcc gtcgaggatg aacacgccgc gcgcgcccag gttcggcgcg gcctggcagc
      421 tggcggacag cgcgagccgg acgcagcatg ccgcgcggcc gggaggaatc gggacgggcg
      481 tctcgcgcgc gcgacgcccg cgcaggcgct cagcgcaggt tgccggtatg gcccagcgag
      541 tatcggcccg gctgcggcca gaccgtcagg ccgtgcggct cctggccgac cgggatctcg
      601 cgcacgtcgc cattggccgt gtcgaagcgg tacacgacgt cgtcgtagcg gcccgagagc
      661 cacaggtact tgccgtcggc cgtcacgttg cccatgtcgg ggctgccccc tccggggatt
      721 ttccagttgg cgacgatctt gttggtcgcg aagtccagca ccgagacgct gcccgggccg
      781 tggcgcgggc cgtggatgcg atgcgatccg cggttggcga tgtacagctt cgtgccgtcg
      841 cggctcgggt acaggccgtg ggcggcgacg ccggtcggga tgaaccccgt ctcggtgaag
      901 ctggcggcgt cgagcaggtg cacgccgtcg gcttccatgt cggcgacgta gagcgtgcgg
      961 ccgtcgggcg agaagcggat gtcctgaggc atgcccttct tcgacgtaca gatctccgtc
     1021 gtcggcgcgc cctcgggcac cgcgtggtcc ttcaggcgcg tggccggcat cttcagcacg
     1081 aggtagccgt cgaccttgcg gttcacgagg tcgatcctgg cgatcgagcc gtcgaactcg
     1141 caggtgaaga acgcggtgcg gccgtcgatc gagaagtcgg cgtggttgat gccgccgcag
     1201 tgcggggcgt cgatcgagta ctgcagcgcc atcgacttcg ggtcgcggaa gtcgagccgg
     1261 tggcgcgcct cggcgacgac gatcgccgac ttgccgtccg gggtgaagta catgttgtac
     1321 gggtcgtcga ccgggatcgc cgtgccgggc ttgccggtgc gcgggtcgat cggcgtcagg
     1381 ctgccgttgc tgctgcgctc ggcattgttg gcggcccaga gcgtgcgcag gtcgtagccc
     1441 acgacgacgt gctgcgggct ctggccgacc ttgaaggtgt cgacgacctt catcttcgcg
     1501 gggtcgatca cgctcaccga gttgccgcgc aagttgggca cgtacacgcg cacgaggtcg
     1561 ccctgcagag cggggctgat cttggcgggg ccgtccgtct cgctgtacag gttgcgcgga
     1621 tcgaccaccg gcggcatgcc ggggacggtc gcgatcgcgg ccggcgcggc gctggccgcg
     1681 ggcgc
//
LOCUS       DASVLN010000305         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_230115, whole genome shotgun sequence.
ACCESSION   DASVLN010000305 DASVLN010000000
VERSION     DASVLN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230115"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VF453_07470"
     CDS             complement(<1..554)
                     /locus_tag="VF453_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7687528.1"
                     /translation="MTALLSSPSTWVALAVLAALAALAAVVNHRLLRLSPTVGAMAVS
                     LAASLALAGLAALGVDGGLLAGERALLARVDFPTLVLQGLLSMLLFAGALQVDVGALR
                     EQRLPVLGFAVAGTAGSTALVGVGLFFVLRALSLDVPIGHCLVFGALVSPTDPVAVIA
                     MLRSARAPRALEAVIAGESLFNDGV"
     gene            complement(628..1998)
                     /gene="pmbA"
                     /locus_tag="VF453_07475"
     CDS             complement(628..1998)
                     /gene="pmbA"
                     /locus_tag="VF453_07475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEX7687529.1"
                     /translation="MATNENQGFAFKPEQFQQIVEDVLAIARQVGASDAATEVSEGAG
                     LSVSVRKGEVENVERNRDKSVGVAIYIGRRRGNASTSDFSRAALEQTVRAAYDIARFT
                     AEDPAAGLPDPADLATPEEAARPLDLFHPWDIDVAGATEIAKRCEDAAFATDRRITNS
                     EGAGVSAQQSHFWMGNSRGFRGGYASSRHSLSVAPIAGKGDAMQRDHWYSSMRDAAEL
                     ADPEAVGRYAAERALSRLNSRKVPTCEVPVLFESTLAAGLLGAFAQGVSGGALYRKAS
                     FLQDSLGTAVFPKDVDILEFPHEPKGKGSAPFDDEGVRTRAREVVDGGVVQGYFLSSY
                     SARKLGMKTTGHAGGSQNLRLTHRRTQPGDDLPEMLRKLDRGLFVIELMGQGVNYVTG
                     DYSRGAAGFWVEGGRIVHPVEEVTIAGHLRAMFAGIQAVGADAYTYGSKTIGSVLLSP
                     MKVAGS"
     gene            2109..2705
                     /gene="yjgA"
                     /locus_tag="VF453_07480"
     CDS             2109..2705
                     /gene="yjgA"
                     /locus_tag="VF453_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX7687530.1"
                     /translation="MPREPRHASNRQAAIDGDLPSRTQKKQQMHDLQALGEELAALPE
                     DRLAKLKIEDRLRDAIAEMKRVRSFEGRRRQLQYIGKLMKFEEVEPLREAVAGFRSGG
                     AKETLALHQAEFWRDRLIAEDDALDGWIAAHPTTDVQQLRSLVRSARKEKLEPGERHG
                     RAYRELFQLVKGHLQDAAEPDDGTDADADADHDPSHDD"
     gene            2698..3297
                     /gene="mog"
                     /locus_tag="VF453_07485"
     CDS             2698..3297
                     /gene="mog"
                     /locus_tag="VF453_07485"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEX7687531.1"
                     /translation="MTDAAPFDPVRIGLVSISDRASAGVYEDKGLPALREWLARALRN
                     PLAFEERLIADDAPTISATLRALVDDARCDLVLTTGGTGPAPRDVTPEATLAVADREI
                     PGFGEQMRQISLRFVPTAILSRQVGVIRGRCLVLNLPGQPKAIAETLQGLPDASPPAP
                     GIFAAVPYCVDLVGGPYIETDAAVCAAFRPKSAQRPPRA"
BASE COUNT          518 a         1320 c         1214 g          421 t
ORIGIN      
        1 acgccgtcgt tgaacaggct ctcgccggcg atcacggcct cgagcgcacg cggtgcgcgc
       61 gccgatcgca gcatcgcgat gacggcgacc ggatccgtcg gggagacgag cgcgccgaag
      121 acgaggcagt gcccgatcgg cacgtccagc gacagcgccc ggagcacgaa gaacaggccg
      181 acgccgacga gcgccgtcga gcccgccgtc cccgccacgg cgaagcccag cacgggcagg
      241 cgctgctcgc gcagagcgcc gacgtccacc tgcagcgcgc cggcgaacag cagcatcgac
      301 agcaggcctt gcagcaccag tgtcgggaaa tccacccggg cgaggagagc gcgctcgccc
      361 gcgagcaggc cgccgtcgac gcccagcgcc gccaggccgg ccagcgcgag cgacgccgcg
      421 agcgacaccg ccatcgcgcc gacggtgggc gacaaacgca ggagccgatg attgacgacc
      481 gccgccagtg cggccagcgc cgccagcacg gcaagcgcga cccaggtgga cggggacgag
      541 agcagcgccg tcaccggcgc gggctccgtg ccgcgaaccg gcgtgcccgg ccgccgaccg
      601 cgggcatcgt ggatccgtgc atgctgctca ggaacccgcc accttcatcg gcgacagcag
      661 caccgagccg atcgtcttcg atccgtaggt gtacgcgtcc gcccccacgg cctggatgcc
      721 ggcgaacatc gcgcgcaagt ggccggcgat cgtcacctcc tcgaccggat ggacgatgcg
      781 gccgccctcg acccagaagc cggccgcgcc gcgcgagtag tcgccggtca cgtagttcac
      841 gccctgcccc atcagctcga tgacgaacag gccccggtcc agcttgcgca gcatctcggg
      901 caggtcgtcg cccggctgcg tgcggcggtg ggtcagtcgc aggttctgcg agccgccggc
      961 atgccccgtg gtcttcatgc ccagcttgcg cgccgaatag ctcgagagga agtagccctg
     1021 caccacgccg ccgtcgacga cctcgcgcgc gcgggtgcgc acgccctcgt cgtcgaaggg
     1081 ggcgctgccc ttgcccttgg gctcgtgcgg gaactcgagg atgtcgacgt ccttcgggaa
     1141 cacggcggtg cccaggctgt cctgcaggaa gctcgccttg cggtacagcg cgccgccgct
     1201 gacgccctgc gcgaacgcgc ccagcaggcc ggcggcgagc gtcgactcga acagcaccgg
     1261 cacctcgcag gtcggcacct tgcgcgagtt caggcgcgag agcgcgcgct ccgcggcgta
     1321 gcggccgacc gcctccggat cggcgagctc ggcggcgtcg cgcatcgagc tgtaccagtg
     1381 gtcgcgctgc atcgcgtcgc ccttgccggc gatgggcgcg accgacagcg agtggcgcga
     1441 gctcgcgtag ccgccgcgaa agccgcgcga gttgcccatc cagaagtgcg actgctgcgc
     1501 cgacacgccc gcgccctcgg agttggtgat gcggcggtcg gtggcgaagg cggcgtcctc
     1561 gcagcgcttg gcgatctccg tggcgccggc gacgtcgatg tcccacggat ggaacaggtc
     1621 cagcgggcgc gcggcctcct cgggcgtcgc caggtcggcg ggatcgggca ggccggcggc
     1681 cggatcctcc gcggtgaagc gcgcgatgtc gtaggccgcg cgaaccgtct gctccagcgc
     1741 ggcgcgcgag aagtccgagg tgctcgcgtt gccgcgccgg cggccgatgt agatcgccac
     1801 gccgaccgac ttgtcgcggt tgcgctcgac gttctcgacc tcgcccttgc gcaccgacac
     1861 cgacaggccc gcgccctcgg acacctcggt ggccgcgtcg ctcgcgccga cctgccgggc
     1921 gatcgcgagg acgtcctcga cgatctgctg gaactgctcc ggcttgaacg caaaaccctg
     1981 gttctcgttg gtggccatcg tgatctctgg ttcgacttgt gaagactgcc gcgcaccgct
     2041 ggtcggcggg cggcgacatt tgcgggacaa tccgggccat catagccaca gccccgactc
     2101 ccctgcacat gccccgcgag ccccgccacg cctccaaccg ccaggccgcc atcgacggcg
     2161 acctgccgag ccggacgcag aagaagcagc agatgcacga cctgcaggcg ctgggcgagg
     2221 agctcgcggc gctgccggag gatcgcctcg ccaagctcaa gatcgaggat cgcctgcgcg
     2281 acgccatcgc cgagatgaag cgcgtgcgct cgttcgaggg caggcggcgc cagctgcagt
     2341 acatcggcaa gctgatgaag ttcgaggagg tcgagccgct gcgcgaggcc gtcgccggct
     2401 tcaggagcgg cggcgcgaag gagacgctcg cgctgcacca ggccgagttc tggcgcgacc
     2461 gcctgatcgc cgaggacgac gccctggacg gctggatcgc cgcgcacccg acgaccgacg
     2521 tccagcagct gcgcagcctc gtgcgctccg cgcgcaagga aaagctcgag cccggagagc
     2581 gccacggccg cgcctaccgt gaacttttcc agctggtcaa ggggcacctg caggacgcgg
     2641 cggagccgga cgacggcacc gatgccgacg cggacgccga ccacgacccg tcgcacgatg
     2701 actgacgccg cgccgttcga tcccgtccgc atcgggctcg tctcgatcag cgaccgcgcc
     2761 tcggccggcg tgtacgagga caagggcctg cccgcgctgc gcgagtggct ggcgcgcgcg
     2821 ctgcgcaatc cgctcgcctt cgaggagcgc ctgatcgccg acgacgcgcc gacgatcagc
     2881 gcgacgctgc gggcgctggt cgacgacgcg cgctgcgacc tcgtgctgac caccggcggc
     2941 accggccccg cgccgcgcga cgtcacgccc gaggcgacgc tggccgtggc cgaccgcgag
     3001 atcccgggct tcggcgagca gatgcgccag atctcgctgc gcttcgtgcc gaccgcgatc
     3061 ctgtcgcggc aagtgggcgt catccgcggc cgatgcctgg tgctgaacct gcccggccag
     3121 cccaaggcga tcgccgagac gctgcaaggc ctgcccgacg cgagcccgcc ggcgcccggc
     3181 atcttcgccg cggtgcccta ttgcgtcgac ctggtcggcg gtccctacat cgagaccgac
     3241 gcggccgtct gcgcggcctt ccggccgaag tcggcccaac gcccgccgcg cgcctgaagg
     3301 cgccggagcg ccgcgtcccg aggatctccc gcacgccagg aacgccgacc cggcgcgccc
     3361 tgccgaagaa gacgaaccgc ccgccagccc gccgccctcc atgcagccga cgtcccatcg
     3421 cacgcccgtc ccccgcgccg ccctgccgct ggcgctcgcc gccgcgctcg cct
//
LOCUS       DASVLN010000306         4526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_500606, whole genome shotgun sequence.
ACCESSION   DASVLN010000306 DASVLN010000000
VERSION     DASVLN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500606"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VF453_07490"
     CDS             complement(<1..978)
                     /locus_tag="VF453_07490"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX7687532.1"
                     /translation="MDAFLPHRLVPRALRAIVALAGACLAAGIAQAQSVEDPPGRVGR
                     VAAVDGDVRTVTSDGSWVSVAANRPLSTGDRITTDHAGHATLEFGSSSFRLGPDSDVT
                     VKQLDDLRMRIRFEQGELAVRVRSADILGELGVDTDEGLWVPHHVGLFRFDRPKRGGL
                     LAGQATAGDMLLQAPDSSLPVAAGQRAQVWREGAQETTHYHMVGVVDDAFTAWVRAQD
                     RQDDADDAAAPKAISSQAALAEMTGLRALHRAGRWSELPGVGPAWTPTRTATGWAPLR
                     EGAWSFASPWGWTWTDAAPWGFAPSHYGRWLHVQGRWAWTPGRWTTRPIY"
     gene            complement(1057..1593)
                     /gene="def"
                     /locus_tag="VF453_07495"
     CDS             complement(1057..1593)
                     /gene="def"
                     /locus_tag="VF453_07495"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7687533.1"
                     /translation="MTVREILKMGDPRLLRVAQPVERFDTPELRGLIADMFETMAHAN
                     GAGLAAPQIGVDLQLVIFGFDRNERYPDAPAVPKTVLINPTITPIGDAEVEGWEGCLS
                     VPGLRGVVPRWERIRYTGVDPQGHVIDREAEGFHARVVQHEVDHLLGRLYPTRMRDLT
                     RFGYTSVLFPEMDPNSDD"
     gene            complement(1644..3770)
                     /gene="ligA"
                     /locus_tag="VF453_07500"
     CDS             complement(1644..3770)
                     /gene="ligA"
                     /locus_tag="VF453_07500"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7687534.1"
                     /translation="MSATPATIPAAAARRAEELHTILHEHAYRYYVLDEPTIPDAEYD
                     KLFQELQALEAQYPDLARADSPTQRVIGQVLEGFAPVRHVVPMLSIRTETDTTEEGAR
                     AFDARVRRELKLAESDPPVVYSAELKFDGLAMNLRYERGVLVQAATRGDGEVGEDVTS
                     NIRTVRQIPLRLRGVDAPVLEVRGEVYMRRDDFEAYNERQRALIAAGAKHEKTVVNPR
                     NAAAGAVRQLDSSQSRRRRLSFFAYGLGEVQGWEVPATHSGLLDALDAMGFPVARQRV
                     LSEGADGLVAFHARIAAERDRLPYDIDGVVYKVDSRELQARLGFVSREPRWAVAHKYP
                     AQEQMTTLLDIDVQVGRTGKLTPVAKLQPVFVGGTTVSNATLHNEGEARRKDVRVGDT
                     VIVRRAGDVIPEVVGVVLERRTPEVAARPLFDMNAKVHGKCPVCGSPIAREEDEADWR
                     CTGGLTCPAQLKAAFLHFASRRAVEIEGFGDKIVDQVVDLGIARTLPEIYKLGFAKLA
                     QLERMGEKSAQKLVEAIEKSKQTTLPRFLYGLGIRHVGESTARDLARHFGDVDKLMDA
                     SVEQLLEVPDVGPIVARSVRAFFDQPHHREVVEQLRAAGITWPAIEGTASDAWRPLLG
                     RTLVLTGTLPTLSRDEAKELIEAAGGKVSGSVSKKTSWVVAGEEAGSKLDKANELGVP
                     VLDEAGLQALLAAPRDAVDAADGPAA"
     gene            complement(3820..>4526)
                     /locus_tag="VF453_07505"
     CDS             complement(3820..>4526)
                     /locus_tag="VF453_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687535.1"
                     /translation="TARGFKPQPWDQAILALRQAGCHREAEDVAIAKERAQHAGERGP
                     VRLLRALWDVSVRFGYRPLRLLWLVPAVWAVSGVLFEFAADAGVMAPTDDKVLSAAEF
                     AHCRPERGGNWARCALAPAYPDFHPWAYAAQAMAPVLELRQAKDWAPVPWVRRQVSTP
                     AAMASDASSPAPAPAPAPASASAAASEQPEATPPTSALGMVALRWSWFESAIGLGVPA
                     LVVWAVSGLVRRKAAG"
BASE COUNT          569 a         1684 c         1652 g          621 t
ORIGIN      
        1 gtagatcggc cgcgtggtcc agcgccccgg cgtccatgcc caccggccct gcacgtgcag
       61 ccagcggccg tagtgcgagg gcgcgaagcc ccacggcgcc gcgtccgtcc acgtccagcc
      121 ccagggcgac gcgaagctcc aggcgccttc gcgcagcggc gcccagccgg tggcggtgcg
      181 cgtcggcgtc caggccggcc cgacgcccgg cagctcgctc cagcggccgg cgcggtgcag
      241 cgcccgcagg ccggtcatct cggcgagcgc cgcctgcgac gagatcgcct tcggcgcggc
      301 cgcgtcgtcg gcgtcgtcct gccggtcctg cgcgcgcacc caggcggtga acgcgtcgtc
      361 gacgacgccg accatgtggt agtgcgtcgt ctcctgcgcg ccttcgcgcc agacctgcgc
      421 gcgctggccc gcggccacgg gcagggagct gtccggggcc tgcagcagca tgtcgccggc
      481 ggtcgcctgc ccggcgagca ggccgccgcg cttcggacgg tcgaagcgga acaggccgac
      541 atggtgcggc acccacaggc cctcgtcggt gtcgacgccc agctcgccga ggatgtccgc
      601 cgaacgcacg cgcaccgcga gctcgccctg ctcgaagcgg atgcgcatgc gcaggtcgtc
      661 gagctgcttg accgtcacgt cggaatcggg cccgagccgg aagctcgacg agccgaactc
      721 cagcgtcgcg tggccggcgt ggtcggtggt gatgcggtcg cccgtcgaca gcggccggtt
      781 ggcggcgacg gagacccagc tgccgtcgct cgtcaccgtg cgcacgtcgc cgtcgaccgc
      841 cgcgacccgg ccgacgcggc cgggcggatc ctccaccgac tgcgcctgcg cgatgcccgc
      901 ggcgaggcag gcgcccgcga gcgccacgat cgcgcgcagg gcgcggggga caagccggtg
      961 cgggaggaag gcgtccatgg aatctcaacg cgcggtgcgc tggccggggt gaccgcaggc
     1021 ggcaatccgc gtgctcggcc gcccttttca ggggactcag tcgtcggagt tcggatccat
     1081 ctcggggaac agcacgctcg tgtagccgaa gcgcgtgagg tcgcgcatgc gcgtcgggta
     1141 cagccgcccg agcaggtggt cgacctcgtg ctgcacgacg cgcgcgtgga agccctcggc
     1201 ctcgcggtcg atgacgtgcc cctgggggtc gacgccggtg tagcggatcc gttcccaacg
     1261 cggcacgacc ccccgcaggc ccggcaccga caggcagccc tcccagccct cgacctccgc
     1321 gtcgccgatc ggcgtgatcg tcgggttgat cagcaccgtc ttgggcacgg cgggcgcgtc
     1381 gggatagcgc tcgttgcggt cgaagccgaa gatcacgagc tgcaggtcga cgccgatctg
     1441 cggcgccgcg agcccggcgc cgttggcgtg cgccatcgtc tcgaacatgt cggcgatcag
     1501 cccgcgcagc tccggggtgt cgaaccgctc gacgggctgg gcgacgcgca gcaggcgcgg
     1561 gtcgcccatc ttgaggattt ctcggacggt catggtcggt cgtgctcgtt ggaggctcga
     1621 tggtagcgac gcgccggcgg cgatcaggcc gcggggccat cggcggcgtc gaccgcgtcg
     1681 cggggcgccg ccagcagcgc ctgcagcccc gcctcgtcca gcaccgggac gcccagctcg
     1741 ttggccttgt ccagcttgct gcccgcctcc tcgcccgcga cgacccagct cgtcttcttc
     1801 gagaccgagc cgctgacctt gccgcccgcg gcctcgatga gctccttggc ctcgtcgcgc
     1861 gagagcgtcg gcagcgtgcc ggtcagcacg agcgtcctgc ccagcagcgg ccgccaggcg
     1921 tcgctggccg tgccttcgat cgccggccac gtgatgcccg cggcgcgcag ctgctcgacg
     1981 acctcgcggt ggtgcggctg gtcgaagaac gcgcgcacgc tgcgcgcgac gatggggccg
     2041 acgtcgggca cctccagcag ctgctccacg ctcgcgtcca tcagcttgtc gacgtcgccg
     2101 aaatggcgcg ccaggtcgcg cgcggtcgac tcgccgacgt gacggatccc gaggccgtac
     2161 aggaagcgcg gcagcgtcgt ctgcttgctc ttctcgatcg cctcgaccag cttctgcgcg
     2221 gacttctcgc ccatgcgctc cagctgcgcg agcttggcga agccgagctt gtagatctcg
     2281 ggcagcgtgc gcgcgatgcc caggtcgacg acctggtcga cgatcttgtc gccgaagccc
     2341 tcgatctcga ccgcgcgccg gctcgcgaag tgcaggaacg cggccttgag ctgcgccgga
     2401 caggtcaggc cgcccgtgca gcgccagtcg gcctcgtcct cctcgcgcgc gatcgggctg
     2461 ccgcagaccg ggcacttgcc gtgcaccttc gcgttcatgt cgaacagcgg ccgcgccgcg
     2521 acctccggcg tgcggcgctc caggacgacg cccacgactt ccgggatcac gtcgcccgcg
     2581 cggcgcacga tcacggtgtc gccgacgcgc acgtccttgc gccgcgcctc gccctcgttg
     2641 tgcagcgtcg cgttgctgac cgtcgtgccg ccgacgaaga ccggctgcag cttggccacc
     2701 ggcgtgagct tgcccgtgcg gccgacctgc acgtcgatgt ccagcagcgt ggtcatctgt
     2761 tcctgcgcgg ggtacttgtg cgcgaccgcc cagcgcggct cgcggctcac gaagccgagc
     2821 cgggcctgca gctcgcgcga gtcgaccttg tagacgacgc cgtcgatgtc gtacggcaag
     2881 cggtcgcgct cggccgcgat gcgggcatgg aacgcgacca ggccgtcggc cccctcgctg
     2941 agcacgcgct gcctggcgac cgggaagccc atcgcgtcga gcgcgtcgag caggccggag
     3001 tgcgtggccg gcacctccca gccctgcact tcgccgaggc cgtaggcgaa gaagctcagg
     3061 cgccggcggc gcgactggct cgagtcgagc tgccgcacgg cgcccgcggc ggcgttgcgc
     3121 gggttgacga cggtcttctc gtgcttcgcg ccggcggcga tcagggcccg ctggcgctcg
     3181 ttgtaggcct cgaagtcgtc gcgtcgcatg tagacctcgc cccgcacctc cagcacgggc
     3241 gcgtcgacgc cgcgcaggcg cagcgggatc tgccggacgg tgcggatgtt ggacgtcacg
     3301 tcctcgccga cctcgccgtc gccgcgcgtg gccgcctgca ccagcacgcc gcgctcgtag
     3361 cgcaggttca tggccaggcc gtcgaacttg agctcggcgg aatagaccac cggcggatcg
     3421 gactcggcca gcttgagctc gcgccgcacc cgcgcgtcga aggcgcgggc gccctcctcg
     3481 gtggtgtcgg tctcggtgcg gatgctgagc atcggcacca cgtggcgcac gggcgcgaag
     3541 ccctcgagca cctgcccgat cacgcgctgc gtgggcgagt cggcgcgcgc gagatccgga
     3601 tactgcgcct cgagcgcctg cagctcctgg aacagcttgt cgtactccgc gtccgggatc
     3661 gtcggctcgt cgagcacgta gtagcggtac gcgtgctcgt ggaggatcgt gtgcagctcc
     3721 tcggcacgcc gggcggccgc ggcggggatg gtggcggggg tggcgctcat ggcggggagt
     3781 gtagtcaggt cgaggcgggc aacgctcgcc cggcccgcct catcccgccg ccttgcgccg
     3841 caccagcccc gacaccgccc acaccaccag cgccggcacg cccaggccga tcgccgactc
     3901 gaaccagctc cagcgcagcg cgaccattcc cagcgcggag gtgggcggcg tcgcctccgg
     3961 ttgctccgag gctgccgcgg acgcggacgc tggcgctggc gctggcgctg gcgctggcga
     4021 ggatgcgtcc gatgccatcg ccgcgggcgt ggatacctgc cgccgtaccc acggcaccgg
     4081 cgcccagtcc ttggcctggc gcagctccag caccggcgcc atcgcctgcg cggcgtaggc
     4141 ccagggatgg aagtcgggat aggccggcgc gagcgcgcag cgggcccagt tgccgccgcg
     4201 ctcgggccgg cagtgcgcga actccgcggc cgacagcacc ttgtcgtcgg tgggtgccat
     4261 gacgccggcg tcggcggcga actcgaacag cacgcccgag accgcccaga cggccggcac
     4321 gagccacagc agccgcagcg ggcggtagcc gaagcgcacc gacacgtccc acagcgcgcg
     4381 cagcaggcgc acggggccgc gctcgccggc gtgctgggcg cgctccttgg cgatggcgac
     4441 gtcctcggcc tcgcggtggc agccggcctg ccgcagggcg aggatcgcct ggtcccaggg
     4501 ctgcggcttg aagccgcggg cggtcg
//
LOCUS       DASVLN010000307         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_338192, whole genome shotgun sequence.
ACCESSION   DASVLN010000307 DASVLN010000000
VERSION     DASVLN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338192"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1661)
                     /locus_tag="VF453_07510"
     CDS             complement(<1..>1661)
                     /locus_tag="VF453_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX7687536.1"
                     /translation="IARRENMRLLGPNSLGFQRPRRGLNASAAGPLARAGSLGVVCQS
                     GALTAAMLDWARTNAVGFSLIAAVGPHTEIDVAQVLDYLAHDPQTQSILVHLEGITGA
                     RRFMSALRSAAIAKPVVVLKAGRERAGTEAAQTHSAASVGSDEVFDAALRRAGAVRVR
                     SFVELFSAAKCLASRYRPVGPRLAIVTNGGGPGVLAADWANRIGLELGKLSPAAAEAL
                     KPQLAECASVADLVDLSEDATPEHYAAAIRAANADPGVDGMLAIHSPKAGGDPVAAAS
                     GVAAVLPQLHKPLLACWMGDATVAAARTLLNDGGVASFRTPEAAVGAFGNIATFYQNQ
                     RLLQQTPPPLSALAEPDLEGARLLVESVLAERRKVLTEMESKTLLSAFHVPVTQTLLA
                     RSPTEAMMIATQLGFPVALKIDSPDIVHKSDVQGVALNVMSGASARDTYVDMIERVRR
                     LAPDAAINGVTVQKMARARRGREVSVGVVTDDPFGPVIVFGAGGVMIELIADRAMELP
                     PLNQFLARRLVERARVARTLGEWRGASAADVAALERVLLRVSEMV"
BASE COUNT          218 a          619 c          617 g          207 t
ORIGIN      
        1 acaccatttc ggagacgcgc agcagcacgc gctcgagcgc ggcgacgtcg gccgcgctcg
       61 cgccccgcca ctcgcccagc gtgcgcgcga cgcgcgcgcg ctcgacgagc cggcgcgcga
      121 ggaactggtt gagcggcggc agctccatcg cgcggtcggc gatcagctcg atcatcacgc
      181 cgcccgcgcc gaacacgatg accggaccga acgggtcgtc ggtgacgacg cccaccgaca
      241 cctcgcgccc gcgccgcgcc cgcgccatct tctgcacggt cacgccgttg atcgcggcgt
      301 cgggcgcgag gcggcgcacg cgctcgatca tgtcgacgta ggtgtcgcgc gcgctcgcgc
      361 cgctcatcac gttgagcgcg acgccctgga cgtcggactt gtgcacgatg tcgggcgagt
      421 cgatcttgag cgcgactggg aagcccagct gcgtcgcgat catcatcgcc tcggtcggcg
      481 agcgggcgag cagcgtctgc gtcaccggca cgtggaaggc cgacagcagc gtcttcgact
      541 ccatctcggt gagcaccttg cggcgctcgg ccagcacgct ctcgaccagc aggcgcgcgc
      601 cctcgaggtc gggctcggca agcgccgaca gcggcggcgg cgtctgctgc agcaggcgct
      661 ggttctggta gaaggtcgcg atgttgccga aggcgccgac ggcagcctcg ggcgtgcgga
      721 agctcgcgac gccgccgtcg ttgagcagcg tgcgcgccgc cgcgacggtg gcgtcgccca
      781 tccagcacgc gagcagcggc ttgtgcagct gcggcagcac cgcggccacg ccgctcgccg
      841 cggccaccgg atcgccgccc gccttgggcg agtggatggc gagcatgccg tcgaccccgg
      901 gatccgcgtt ggcggcgcgg atggcggcgg cgtagtgctc gggcgtcgcg tcctcggaca
      961 ggtcgacgag atcggccacc gacgcgcact cggccagctg cggcttgagc gcctcggccg
     1021 cggccggcga cagcttgccc agctcgagcc cgatccggtt ggcccagtcg gccgcgagca
     1081 cgccggggcc gccgccgttg gtgacgatgg ccaggcgcgg cccgaccggc cgatagcgcg
     1141 acgccaggca cttcgccgcc gagaacagct cgacgaacga gcgcacccgc accgcacccg
     1201 cgcgccgcag cgcggcgtcg aagacctcgt cgctgccgac gctcgcggcg ctgtgggtct
     1261 gcgcggcctc ggtgccggcg cgctcgcggc cggccttgag cacgaccacc ggcttggcga
     1321 tcgcggccga gcgcagcgcg ctcatgaagc ggcgcgcgcc cgtgatgccc tcgaggtgca
     1381 cgaggatgct ctgggtctgc gggtcgtggg cgaggtagtc gagcacctgg gcgacgtcga
     1441 tctcggtatg cggcccgacc gcggcgatga gcgagaagcc gacggcgttc gtgcgcgccc
     1501 agtccagcat cgccgccgtc agcgcgcccg actggcacac cacgcccagc gaccccgcac
     1561 gcgcgagcgg cccggcggcg ctcgcgttga gcccgcgccg cggccgctgg aagccgaggc
     1621 tgttggggcc gagcaggcgc atgttctcgc gccgcgcgat g
//
LOCUS       DASVLN010000308         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_500679, whole genome shotgun sequence.
ACCESSION   DASVLN010000308 DASVLN010000000
VERSION     DASVLN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500679"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VF453_07515"
     CDS             <1..265
                     /locus_tag="VF453_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7687537.1"
                     /translation="AAPGAGARLACSDPDDQKFIDLAIAARADVLVTRDKALLKLARR
                     AAERHGVRVCRPPEWSRLAAGEPGQSASACQSGMPKSWRPSRV"
     gene            complement(205..>1551)
                     /locus_tag="VF453_07520"
     CDS             complement(205..>1551)
                     /locus_tag="VF453_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HEX7687538.1"
                     /translation="TGAAAVAPVAPDSPPVLPRRSVGGAVLEVIGFDLALSNLDRAFS
                     RSHDYDVSFHSIEHNLRGPWVTDSDPFQINQFGHPYQGSIYHNIGRSMGLDYWQASAL
                     TFGGSVWWEITGEKTPPSRNDQVASGIAGSFLGEPLFRMSHLVADHSSLPPSWRPWLA
                     AAISPAAGANRWAFGSAYGGEFDDHDPAYYSRLRVGAARATQTQFDASEPYHRETAEV
                     DYAMDYGLPGKPGYRYDRPFDYFDFELQLSSANGVENLTSNGLLLGDRYALGDAVRGV
                     AGLYGNYEYLSPQIFHVSSTGVSIGTTLQWRIAPAFALQGSVLGGVGYSAASTTLRDV
                     DSKEYHYGMAPRASGNLRLVVAQRAAVDVAARLVSLGRIANRGAGRDDISRIESAFTW
                     RLAGPHAIGMTYAWSHRSAAFAGGAERKQTLGQVGIFYTLLGRQDFGIPDWHADAD"
BASE COUNT          226 a          536 c          578 g          211 t
ORIGIN      
        1 ggccgcccca ggcgccggcg cgcggctcgc ctgcagcgat cccgacgacc agaagttcat
       61 cgacctcgcg atcgccgcgc gagccgatgt gctcgtgacc cgcgacaagg ccctgctgaa
      121 gctcgcgcgg cgcgccgcgg agcgccatgg cgtgcgcgtc tgccggccgc ccgaatggtc
      181 gcggctggcg gccggcgaac ccggtcagtc agcgtcggcg tgccagtcgg ggatgccgaa
      241 atcctggcgg ccgagcaggg tgtagaagat gccgacctgc cccagcgtct gcttgcgctc
      301 ggcgccgccc gcgaacgcgg cgctgcggtg cgaccaggcg taggtcatgc cgatcgcgtg
      361 cggccccgcg aggcgccagg tgaacgccga ctcgatgcgc gagatgtcgt cgcgccccgc
      421 cccgcggttg gcgatccggc cgagcgacac gaggcgcgcg gcgacgtcga cggcagcgcg
      481 ctgcgccacg accaggcgca ggtttcccga cgcgcgcggc gccatgccgt agtggtattc
      541 cttggagtcg acgtcgcgca gcgtcgtgct ggccgccgaa tagccgaccc cgcccagcac
      601 cgagccctgc agcgcgaacg ccggcgcgat gcgccactgc agcgtggtgc cgatcgagac
      661 gccggtgctc gagacgtgga agatctgcgg cgagaggtac tcgtagttgc cgtacaggcc
      721 cgcgaccccg cgcaccgcgt cgccgagcgc gtagcggtcg cccagcagca ggccgttgga
      781 cgtgaggttc tcgacaccgt tggcgctgga caactgcaac tcgaagtcga aatagtcgaa
      841 cggccggtcg tagcggtagc ccggcttgcc gggaaggccg tagtccatcg cgtagtcgac
      901 ctccgccgtc tcgcggtgat aaggctcgct cgcgtcgaac tgggtctgcg tcgcgcgcgc
      961 ggcaccgacg cgcagccggc tgtagtaggc cgggtcgtgg tcgtcgaatt cgccgccgta
     1021 cgccgagccg aacgcccagc ggttggcacc cgccgcgggc gagatcgccg ccgccagcca
     1081 gggccgccac gacggcggca ggctgctgtg gtcggcgacg aggtgcgaca tgcggaacag
     1141 cggctcgccg aggaagctgc cggcgatgcc gctggccacc tggtcgttgc gggacggcgg
     1201 cgtcttctcg ccggtgatct cccaccagac gctgccaccg aaggtcagcg cggaggcctg
     1261 ccagtagtcc agccccatgc tgcggccgat gttgtggtag atcgagccct ggtacggatg
     1321 gccgaactgg ttgatctgga acggatcgct gtcggtcacc cacgggccgc gcaggttgtg
     1381 ctcgatcgag tggaagctca cgtcgtagtc gtgcgagcgg ctgaaggcgc ggtcgaggtt
     1441 gctgagcgcg aggtcgaagc cgatcacctc gagcaccgcg ccgccgacgc tgcggcgcgg
     1501 aaggacgggc ggcgaatccg gcgcgacagg cgcgacggcg gcggcgcccg t
//
LOCUS       DASVLN010000309         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_15816, whole genome shotgun sequence.
ACCESSION   DASVLN010000309 DASVLN010000000
VERSION     DASVLN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15816"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1827)
                     /locus_tag="VF453_07525"
     CDS             complement(154..1827)
                     /locus_tag="VF453_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7687539.1"
                     /translation="MSPTLPAAEAGIRPRNGLSHVTGRTDLPLSDATIHRLLADTVAR
                     FPDRPAVVFREQGVRWTWREFLAEVEALASGLAALGLSKGDRVGIWSPNRHEWLVTQF
                     ATARMGMILVNINPAYRLSELTYALKVSGCRAVISAERLKTSDYLAMLQKVRPELPDL
                     QHVIRMGNGRTEGMLAYGDVLARGRAEPVEAAPTSCEEPINIQFTSGTTGNPKGATLT
                     HRNVVNNGRFIAMAMDFSERDSLCIPVPLYHCFGMVLAVLASVSTGATMVFPGEAFEP
                     GAALAAVAEERCTALHGVPTMFIAELAHPRFAEFDLGSLRTGIMAGSPCPIETMKQVV
                     SKMHMEQVTIAYGMTETSPVSFQSSTTDPLERRVSTVGRVQPHLECRIVDADGNTVPV
                     GEKGELCTRGYSVMLGYWGDEARTREAVVDGWMHTGDLATIDEEGYCNIVGRVKDMLI
                     RGGENVYPREIEEFLFRHPKVQSVQVFGVPDAKYGEEVCAWIVLKPGESATEDEIRDF
                     CRDQIAHYKIPRYVRFVDELPMTVTGKAQKFVMRERMMAELGIAAAKTA"
     gene            complement(1936..>2450)
                     /locus_tag="VF453_07530"
     CDS             complement(1936..>2450)
                     /locus_tag="VF453_07530"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX7687540.1"
                     /translation="AALAAPAGAEELVSPYGVFVEGGKADDSYSVVVGTAVTWLQPSK
                     WGAGNASGYLELSGGEWTCRKHPGGHLEACSTQLGITPVIRYAPSWWQHWYGEIGIGA
                     DVIFPRYRSDRRVFGSEYNFGDHLGIGRKFGDGGRDEIELRAEHYSNGGYKHPNPGQN
                     WVQVRYMHWF"
BASE COUNT          350 a          878 c          849 g          373 t
ORIGIN      
        1 tccgctgggc gctcacccac ggcgcggcgc aggcgcaggc cctcgcctac gtcgcattgc
       61 ccgcggcggc ggtgcggctg gtggagcaat cgctgcccgc ggctccctga gcgggtcgcg
      121 gccgggcgcg aaccgcggcc cggcgcgggc gcgtcaggcc gtcttcgccg ccgcgatgcc
      181 cagctccgcc atcatgcgct cgcgcatcac gaacttctgc gccttgccgg tgacggtcat
      241 cggcagctcg tcgacgaagc gcacgtagcg cgggatcttg tagtgcgcga tctggtcgcg
      301 gcagaagtcg cggatctcgt cctcggtcgc ggactcgccc ggcttcagca cgatccacgc
      361 gcagacctcc tcgccgtact tggcgtcggg cacgccgaac acctgcaccg actgcacctt
      421 cggatggcgg aacaggaact cctcgatctc gcgcggatag acgttctcgc cgccgcggat
      481 cagcatgtcc ttcacgcgcc cgacgatgtt gcagtagccc tcctcgtcga tcgtcgcgag
      541 gtcgccggtg tgcatccagc cgtcgaccac ggcctcgcgg gtgcgcgcct cgtcgcccca
      601 gtagccgagc atcaccgagt agccgcgcgt gcacagctcg cccttctcgc cgaccggcac
      661 cgtgttgccg tcggcgtcga cgatgcggca ttccaggtgc ggctgcacgc gaccgaccgt
      721 ggagacccgg cgctcgagcg ggtcggtggt cgagctctgg aacgacaccg ggctggtctc
      781 ggtcatgccg taggcgatcg tcacctgttc catgtgcatc ttcgagacga cctgcttcat
      841 ggtctcgatc gggcacggcg agccggccat gatgccggtg cgcagcgagc cgaggtcgaa
      901 ctccgcgaag cgcggatgcg cgagctcggc gatgaacatc gtcggcacgc cgtgcagcgc
      961 ggtgcagcgc tcctcggcga cggccgcgag cgccgccccg ggctcgaagg cttcgcccgg
     1021 gaacaccatc gtcgcgcccg tggacaccga ggccagcacc gccagcacca tgccgaagca
     1081 gtggtacagc ggcacgggaa tgcacagcga gtcgcgctcg ctgaagtcca tcgccatcgc
     1141 gatgaagcgc ccgttgttga cgacgttgcg gtgcgtgagc gtggcgccct tgggattgcc
     1201 cgtcgtgccg ctcgtgaact ggatgttgat cggctcctcg caggacgtcg gcgccgcctc
     1261 gaccggctcc gcgcggccgc gggccagcac gtccccgtag gccagcatgc cctcggtgcg
     1321 cccgttgccc atgcggatca cgtgctgcag gtccggcagc tcggggcgca ccttctgcag
     1381 catggccagg tagtccgagg tcttgagcct ctcggccgag atcaccgcgc gacagcccga
     1441 gaccttcagc gcgtacgtca gctccgacaa ccggtacgcc gggttgatgt tgaccaggat
     1501 catgcccatg cgcgcggtcg cgaactgggt cacgagccat tcgtggcggt tgggcgacca
     1561 gatgcccacg cggtcgccct tggacagccc cagcgcggcc aggcccgagg cgagcgcctc
     1621 gacctcggcc aggaattcgc gccacgtcca gcgcacgccc tgctcgcgga agacgacggc
     1681 ggggcggtcg ggaaagcgcg cgacggtgtc ggcgagcagg cggtggatcg tcgcgtccga
     1741 cagcggcagg tcggtgcggc cggtcacgtg cgacaggccg ttgcgcgggc ggatgccggc
     1801 ttcggcggcg gggagggtgg ggctcatcgt ctcgtctcca ggtcgcgctt cctcggaaac
     1861 gggaagctcg ccggcgatga tgatgcccca accgaacacg aaactgacgc cgggcagacc
     1921 cgccccgccg ccgcttcaga accagtgcat gtagcgcacc tgcacccagt tctggcccgg
     1981 gttgggatgc ttgtagccgc cgttggagta gtgctccgcg cgcagctcga tctcgtcgcg
     2041 gcccccgtcg ccgaacttgc ggccgatgcc gaggtggtcg ccgaagttgt actcgctgcc
     2101 gaagacgcgc cggtcgctgc gatagcgggg gaagatgacg tcggcgccga tgccgatctc
     2161 gccgtaccag tgctgccacc acgagggcgc gtagcggatc accggcgtga tgcccagctg
     2221 cgtcgagcag gcctccagat ggccgccggg atgcttgcgg cacgtccatt cgccgcccga
     2281 cagctcgagg tagccgctcg cgttgccggc gccccacttc gacggctgca gccacgtcac
     2341 cgccgtgccg accaccaccg agtagctgtc atccgccttg ccgccctcga cgaagacgcc
     2401 gtagggcgag accagctcct cggcgccggc cggcgcggcg agcgcggccg
//
LOCUS       DASVLN010000310         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_285061, whole genome shotgun sequence.
ACCESSION   DASVLN010000310 DASVLN010000000
VERSION     DASVLN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285061"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /gene="dprA"
                     /locus_tag="VF453_07535"
     CDS             <1..1024
                     /gene="dprA"
                     /locus_tag="VF453_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX7687541.1"
                     /translation="PDAVAALLAPHPRRDALPDATWHWLAGAPARGPTRDVVVLGDPR
                     YPPLLLETADPPLMLYTLGRVDLLRASALAIVGSRTATPQGLETAHAFAQQLSELGLT
                     IVSGLALGVDAAAHEGGLAGAGRTVAFVGTGIDQVYPAENAALARRIAAEGLVASEYP
                     LGMPPIAANFPRRNRLIAGTAQGTLVVEAALKSGSLITARMALEAGREVFAIPGSIHS
                     QQAYGCHRLIQQGAKLVGAVEDILQELRLPGQGAPRARMWRPPTAGDPSAGSDDGAPA
                     PVPTAAAIPGALGEALGYDPVTFDALAARTGLPSERLAAELLSLELAGAVQRLPGGLF
                     QRRGLA"
     gene            1227..1679
                     /locus_tag="VF453_07540"
     CDS             1227..1679
                     /locus_tag="VF453_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEX7687542.1"
                     /translation="MFDVLVYLYENYWRPDACPDHDQLSRKLTAVGFESDEIQEALHW
                     LDGLADTAQGYTGEQSADAIRLYSPREIEQLGETSLGFVSFLESAGVLPPPMREMVVD
                     RAMAAGGNPLALDDMKVIILMVFWSLGEEPDALILDELFVAPEDRLIH"
     gene            complement(1779..>3383)
                     /locus_tag="VF453_07545"
     CDS             complement(1779..>3383)
                     /locus_tag="VF453_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX7687543.1"
                     /translation="GGYGGAAGAPTGYGGGTGYGGGGYGTGYGRGGRQGPGTGYGGEN
                     RTSEADATLLRFGRTMEKHVHGFMNTTRNVAPSGTLQGALASAQRVFVERVEAKIQEG
                     SSLAPAEMIEALRVQKQAVKQAASTPTERATIEVVAMMFQSILTEERIPASVRVWFAR
                     LQMPVLRVAISEPDFFSSAQHPARRLIDRMGACVMGFDAAPQGALPEIEAEIARVVQV
                     VEAFPETGRKVFQTVLVEFERFLERYFRDHNEITRKGVSLASQVEQRETLAIQYTIEL
                     RKLLDGVPVQDGVRDFLFQVWADVLATTTMRHGAQSDETRSVREAALELVWIASAKTT
                     REERAEVIRRLGPLLAALRQGMLVGGLQAEKQESSLRTLNVALTAAFAARSATIDPDQ
                     LARLKGRLEALDEILPDADFEIDDSFALDLSGHDSDDLEVVSEGGTTPSPAALKASDE
                     MMVGAWYMLEYRERTEPVQLVWQGLHRQLSLFVSANGRCVLFQRRRLAAFLQAHLLTP
                     AQDETLTIAATRSAIDKINADPQKLH"
BASE COUNT          452 a         1238 c         1193 g          500 t
ORIGIN      
        1 gcccgatgcg gtggccgcgc tcctcgcgcc gcatccccgc cgcgacgcgc tgcccgacgc
       61 cacctggcac tggctcgccg gcgcgcccgc caggggcccg acgcgcgacg tcgtcgtcct
      121 cggcgatccg cgctatccgc cgctgcttct ggagacggcc gatcctcccc tgatgctgta
      181 caccctcggt cgcgtcgacc tgctgcgcgc ctccgcgctc gcgatcgtcg gcagccgcac
      241 ggcgacgccg caggggctcg agacggcgca cgccttcgcg cagcagctca gcgagctcgg
      301 gctgacgatc gtctcggggc tcgcgctggg cgtcgacgcg gccgcgcacg agggcggcct
      361 cgccggcgcg gggcgtaccg tcgcgttcgt cggcacgggc atcgaccagg tctatcccgc
      421 cgagaacgcg gcgctggcgc ggcgcatcgc cgccgaaggc ctggtcgcga gcgagtatcc
      481 gctgggcatg ccgcccatcg cggccaactt cccccgtcgc aaccgcttga tcgcggggac
      541 ggcgcagggc acgctggtcg tggaggccgc cctgaagtcg ggctcgctga tcacggcgcg
      601 catggcgctg gaggccggcc gcgaggtgtt cgcgatcccg ggatcgatcc actcgcagca
      661 ggcctacggc tgccatcgct tgatccagca gggcgccaag ctggtcggcg cggtcgagga
      721 catcctgcag gagctgcgcc tgcccggcca aggcgctccg cgggcgcgca tgtggcgacc
      781 cccgacggcc ggagacccct cggcgggatc ggacgacggc gcgccggccc cggtaccgac
      841 cgcggccgcc atccccggcg cgctcggcga ggcgctcggc tacgacccgg tgaccttcga
      901 cgcgctggcc gcccgcaccg gcctgccgag cgagcgcctg gccgcggagc tgctgtcgct
      961 ggaactcgcg ggcgcggtgc agcgcttgcc cggcgggttg ttccagcgcc ggggcctcgc
     1021 ttgagggcgg ccccgcactg tcgagcggcc ggactgccgg tcgagaagcg ggggcggccg
     1081 cgtttgcagg atgcgtgccc acctacgccc gtcgagcaag aaaaccgcgg cttccgaagg
     1141 acgatttcac aaaggtccaa caggggtcgt ctaccccgcc tgttcgcgcg tgccgtgcca
     1201 gggcgctggg gtatagtcgc ctccatatgt tcgacgtcct tgtctacctg tacgaaaact
     1261 actggcgacc ggacgcttgt cccgaccatg accagttgtc gcgcaagctg accgccgtcg
     1321 gtttcgagtc ggacgagatc caggaagccc tgcactggct ggacggcctg gcagacaccg
     1381 cccagggcta caccggcgag caatccgcgg acgccatccg cctgtattcg ccgcgcgaga
     1441 tcgagcaact cggcgaaacc tcgctcggct tcgtgagctt cctggagtcg gcgggcgtgc
     1501 tgcccccgcc gatgcgcgag atggtggtcg accgcgcgat ggccgcaggc ggcaatccgc
     1561 tcgccctgga cgacatgaag gtcatcatcc tgatggtctt ctggagcctg ggcgaagagc
     1621 cggacgcgct gatcctcgac gagctcttcg tcgcgccgga agaccgcctg atccactgac
     1681 ccttcgacgc gcccccgcgc cccgaaacga cgacggctcc cgcgggagcc gttttccatg
     1741 gctgcgcggc agaggcgatg cccgctcgca cgcgatcgct agtgcagctt ctgtggatcc
     1801 gcgttgatct tgtcgatcgc gctgcgcgtc gcggcgatgg tgagggtctc gtcctgggcg
     1861 ggcgtgagca ggtgcgcctg caggaaggcg gcgagccgcc ggcgctggaa cagcacgcag
     1921 cgtccgttgg ccgagacgaa cagcgacagc tgccggtgca gcccctgcca gacgagctgc
     1981 acgggctcgg tgcgctcgcg gtactcgagc atgtaccagg cgccgaccat catctcgtcg
     2041 cttgccttca aggcggcggg cgacggcgtg gtcccccctt ccgagacgac ctcgagatcg
     2101 tccgagtcat ggcccgacag gtcgagcgcg aacgaatcgt cgatctcgaa gtcggcatcg
     2161 ggcaggatct cgtcgagcgc ctcgagccgg cccttcaggc gggcgagctg gtcggggtcg
     2221 atcgtggccg agcgcgcggc gaacgcggcg gtcaacgcga cgttgagggt gcgcagcgag
     2281 ctctcctgct tctcggcctg gagcccgccg accagcatgc cctggcgcag cgccgccagc
     2341 agcgggccca ggcggcggat gacctcggcg cgctcctcgc gcgtcgtctt ggcgctggcg
     2401 atccacacga gctcgagcgc cgcctcgcgc accgagcgcg tctcgtcgct ctgcgcgccg
     2461 tggcgcatcg tcgtcgtggc gagcacgtcg gcccagacct ggaagaggaa gtcgcgcacg
     2521 ccgtcctgca cgggtacgcc gtccagcagc ttgcgcagct cgatcgtgta ctggatcgcg
     2581 agcgtctcgc gctgctcgac ctggctcgcg agcgagacgc ccttgcgcgt gatctcgttg
     2641 tgatcgcgga aatagcgctc gaggaagcgc tcgaactcca ccagcaccgt ctggaagacc
     2701 ttgcggccgg tctccgggaa ggcctcgacc acctgcacga cgcgcgcgat ctcggcctcg
     2761 atctccggca gcgcgccctg cggcgcggcg tcgaagccca tcacgcaggc gcccatgcgg
     2821 tcgatcagcc ggcgcgcggg atgctgggcc gaggagaaga agtccggctc gctgatcgcc
     2881 acccgcagca cgggcatctg caggcgcgcg aaccagacgc gcaccgacgc ggggatgcgc
     2941 tcctcggtga ggatgctctg gaacatcatc gcgacgacct cgatcgtcgc gcgctcggtc
     3001 ggcgtgctgg cggcctgctt gaccgcctgc ttctgcacgc gcagcgcctc gatcatctcg
     3061 gcgggcgcga ggctgctgcc ctcctggatc ttcgcctcga cgcgctcgac gaacacgcgc
     3121 tgcgccgacg ccagcgcgcc ctgcagcgtg cccgagggcg cgacgttgcg cgtcgtgttc
     3181 atgaagccgt ggacgtgctt ctccatcgtg cggccgaagc gcagcagcgt ggcatcggcc
     3241 tcgcttgtgc ggttctcgcc gccgtagccg gtgcccgggc cctggcggcc gccgcggccg
     3301 tagccggtgc cgtagccccc gccgccgtag ccggtgccgc cgccatagcc ggtcggggcg
     3361 ccggcggcac cgccgtatcc gcc
//
LOCUS       DASVLN010000311         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_500722, whole genome shotgun sequence.
ACCESSION   DASVLN010000311 DASVLN010000000
VERSION     DASVLN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500722"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1181
                     /locus_tag="VF453_07550"
     CDS             144..1181
                     /locus_tag="VF453_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEX7687544.1"
                     /translation="MTFTVTLHPAERAFDVARDETILSAAIRAGVGMPYGCRDGACGS
                     CKSRLKEGRVIHGAHQHKALSEAEEAEGFILTCCATPQTDCTVEARVVAGAGEYPITK
                     LPGRVLSLVKPRPDVAIVRIQLPANQAFRYRAGQYVEFILRDGARRSYSMANAPHNLG
                     TPPAIELHLRHMPGGKFTDHVFGAMKEKDILRMEGPFGSFFLREESDRPVVLLASGTG
                     FAPIKALVEQMHHLGSIRPVRLYWGARRPADLYLHEWLDTARTLLPQLECVPVVSDAL
                     PEDGWTGRTGFVHEAVMADLPDLSGHQVYACGAPVMVESARRDFIARCALPEAEFYAD
                     SFTSEADKHGD"
     gene            1259..1687
                     /locus_tag="VF453_07555"
     CDS             1259..1687
                     /locus_tag="VF453_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687545.1"
                     /translation="MLWLSPVHQVVWSLLIGAATFAVASLWPVSAWACARLRVGWFML
                     AAAVTGACVALDLQAARIDVGIDVSRWVAYGFGASWIVLPLYLAFPAAVCIASAQSLR
                     LAGVSPRVARTLALTVAAMAVVVAPFAAIGAACQLTNECF"
BASE COUNT          213 a          663 c          610 g          248 t
ORIGIN      
        1 tcggcacgcc gcgcgcgacg accggggcag gaaggattca tgcggggcat tgtcgatgaa
       61 ggacaatgcg ggccgggcgc gccgccagcg atgcgccgcc gcgccctcgc ccgacggcgc
      121 gcgaagagat ccagcctcct gccatgacct tcaccgtcac cctccatccc gccgagcggg
      181 ccttcgacgt cgcgcgcgac gagaccatcc tctcggcggc gatccgcgcg ggcgtgggca
      241 tgccctacgg ctgccgcgac ggcgcctgcg gatcgtgcaa gagccggctc aaggaaggcc
      301 gcgtgatcca cggcgcgcac cagcacaagg cgctgtccga ggccgaggag gccgagggct
      361 tcatcctcac ctgctgcgcg acgccgcaga ccgactgcac ggtcgaggcg cgcgtcgtcg
      421 ccggcgccgg cgagtacccg atcaccaagc tgcccggccg cgtgctgagc ctcgtgaagc
      481 cgcgtccgga cgtcgccatc gtgcgcatcc agctgccggc caaccaggcg ttccgctacc
      541 gcgccggcca gtacgtggag ttcatcctgc gcgacggcgc gcgccgcagc tactcgatgg
      601 ccaacgcgcc gcacaacctc ggcacgccgc cggccatcga gctccacctg cgccacatgc
      661 cgggcggcaa gttcaccgac cacgtcttcg gcgcgatgaa ggagaaggac atcctgcgca
      721 tggaaggccc gttcggcagc ttcttcctgc gcgaggagag cgaccggcct gtcgtgctgc
      781 tcgcctccgg cacgggcttc gcgccgatca aggcgctcgt cgagcagatg caccacctgg
      841 gcagcatccg gcccgtgcgg ctgtactggg gcgcgcgccg gccggccgac ctctacctgc
      901 acgagtggct ggacaccgcg cgcacgctgc tgccgcagct cgaatgcgtg ccggtcgtct
      961 ccgacgcgct gcccgaggac ggctggaccg gccgcaccgg cttcgtccac gaggcggtga
     1021 tggccgacct gcccgacctc tcgggccacc aggtctatgc ctgcggcgcg ccggtcatgg
     1081 tggagtcggc caggcgggac ttcatcgccc gctgcgccct gcccgaggcc gagttctacg
     1141 ccgacagctt cacctccgag gccgacaagc acggcgactg agcgccgtcc ccgcgcgccc
     1201 gtggccgacg cgcgcggccg cggcgcgcgg tgcgcgcacg ccgcatcctc ccaacccgat
     1261 gctctggctg tcgcctgtcc accaagtggt gtggtcgctg ctgatcggcg ccgccacctt
     1321 cgccgtcgcg tcgctgtggc cggtgtcggc ctgggcctgc gcgcggctgc gcgtcggctg
     1381 gttcatgctc gccgccgccg tcaccggcgc ctgcgtggcg ctcgacctgc aggccgcccg
     1441 catcgacgtc ggcatcgacg tgtcgcgctg ggtcgcctac ggcttcggcg cgagctggat
     1501 cgtcctgccg ctgtacctcg ccttcccggc ggcggtgtgc atcgcctcgg cgcagtcgct
     1561 gcggctggcg ggcgtgtcgc cgcgcgtcgc gcgcacgctg gcgttgacgg tcgcggcgat
     1621 ggccgtcgtc gtcgcgccgt tcgcggcgat cggggcggcg tgccagctga ccaacgagtg
     1681 cttctgagcc tcgcagggtc gcgcaggcga cgggtccgcg tcccgccggt cgcg
//
LOCUS       DASVLN010000312         2055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_69356, whole genome shotgun sequence.
ACCESSION   DASVLN010000312 DASVLN010000000
VERSION     DASVLN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69356"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VF453_07560"
     CDS             complement(<1..334)
                     /locus_tag="VF453_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX7687546.1"
                     /translation="MMTSPLQRKANPRGREIVSVTPEQAGWAHVGFRALRLAAGEAEA
                     LETGDRELCVVVLGGSVDARVDGVAYTALGNRADVFDDAPPAALYVPGGRRVEIVAGA
                     AAEVALCTA"
     gene            complement(331..1278)
                     /gene="iolE"
                     /locus_tag="VF453_07565"
     CDS             complement(331..1278)
                     /gene="iolE"
                     /locus_tag="VF453_07565"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HEX7687547.1"
                     /translation="MTWNVRIGINPISWSNDDLPELGGDTPLETALREGAEIGYEGFE
                     LGNKFPKDAQALKAKLAEYGVACVSGWYSGRLAEGLLESEIAACGAHMSKLAANGCKV
                     VVYGEVAGSIQGQISTPLSRRPRFASDAHWSGYAQRLEQFGRHLKDAYGLTLAYHHHM
                     GAYVESPEDVDRLMQLTGPDVGLLFDTGHAYYGGATDVAAQLRKHLARVVHVHCKDVR
                     PQVVRSARNDGWSFLTGVINGTFTVPGDGVIDFDAVLTLLHGAGYEGWLVVEAEQDPA
                     VAPSYQYAKKGYDTLRAIVDRLDAGDAGDARGARGEKAA"
     gene            complement(1312..>2055)
                     /locus_tag="VF453_07570"
     CDS             complement(1312..>2055)
                     /locus_tag="VF453_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7687548.1"
                     /translation="AASAWNARVTELTTAPRADALPYDADVIGAIRDSAADSPANDIV
                     VCAAGTLPAELHKLWRTSVPGGYHVEYGYSCMGYEIAGGLGVKLAQPEREVVVTVGDG
                     SYLMMNSEIATSVMLGLKLVVVVLDNRGYGCIQRLQQACGGASFNNLLEDCVPEGGTD
                     VRVDFAAHAAAMGAGARHVRDLAELRAALAEARAATTTQVIVIDTTHARTTDDGGAWW
                     EVGIPGVSARPEVGAAAAAALDAKRAQRP"
BASE COUNT          257 a          749 c          752 g          297 t
ORIGIN      
        1 gcgcggtgca cagcgcgacc tcggcggcgg cgcccgcgac gatctcgacc cgccgcccgc
       61 ccggcacgta cagcgcggcg ggcggggcgt cgtcgaagac gtcggcgcgg ttacccagtg
      121 ccgtatacgc gacgccgtcg acgcgcgcgt cgacgctgcc gcccaggacg accacgcaca
      181 gctcgcggtc gccggtctcc agggcctccg cctcgcccgc cgccaggcgc agcgcgcgga
      241 agccgacgtg cgcccagccg gcctgctcgg gcgtgaccga cacgatctcg cgcccgcgcg
      301 ggttcgcctt gcgctgcagc gggctcgtca tcacgcggcc ttctcgccgc gggcgccgcg
      361 ggcgtccccg gcgtccccgg cgtcgaggcg gtcgacgatc gcgcgcagcg tgtcgtagcc
      421 cttcttcgcg tactggtagc tcggcgcgac ggcgggatcc tgctccgcct cgaccaccag
      481 ccagccctcg tagccggcgc cgtgcagcag cgtgagcacg gcgtcgaagt cgatcacgcc
      541 gtcgccgggg acggtgaacg tgccgttgat gacgccggta aggaagctcc agccgtcgtt
      601 gcgcgcgctg cgcacgacct gcgggcgcac gtccttgcaa tgcacgtgca cgacgcgcgc
      661 gaggtgcttc ctcagctgcg cggcgacgtc ggtggcgccg ccgtagtagg cgtggccggt
      721 gtcgaacagc aggccgacgt cggggccggt cagctgcatc aggcggtcga cgtcctcggg
      781 cgactcgacg tacgcgccca tgtggtggtg gtacgcgagc gtcaggccgt aggcgtcctt
      841 gaggtggcgc ccgaactgct ccagccgctg cgcgtagccc gaccagtgcg cgtcgctggc
      901 gaagcgcggc cggcgcgaca gcggcgtcga gatctgcccc tggatcgagc cggcgacctc
      961 gccgtacacc acgaccttgc agccgttggc ggcgagcttg gacatgtgcg cgccgcacgc
     1021 ggcgatctcg ctttccagca ggccttccgc gagacgcccc gagtaccagc cgctgacgca
     1081 cgcgacgccg tactcggcca gcttggcctt gagcgcctgc gcatccttgg gaaacttgtt
     1141 gccgagctcg aagccctcgt agccgatctc cgcgccttcg cgcagcgcgg tctcgagcgg
     1201 cgtgtcgccg ccgagctcgg gcaggtcgtc gttgctccag gagatcgggt tgatgccgat
     1261 gcggacgttc caggtcatgg tgggggctct ctccagtcgt cgaaggggat gtcagggccg
     1321 ctgcgcgcgc ttggcgtcca gggcggccgc cgcggcggcg ccgacctcgg gtcgcgcgga
     1381 aaccccgggg atgccgacct cccaccacgc gccgccgtcg tcggtggtgc gtgcgtgggt
     1441 ggtgtcgatg acgatcacct gcgtcgtcgt cgcggcgcgg gcctcggcca gcgccgcgcg
     1501 cagctcggcg aggtcgcgca cgtggcgggc gccggcgccc atcgccgcgg cgtgggcggc
     1561 gaagtcgacc cgcacgtcgg tgccgccttc gggcacgcag tcctcgagga ggttgttgaa
     1621 gctcgcgccg ccgcaggcct gctgcaggcg ctggatgcag ccgtagccgc ggttgtcgag
     1681 gacgaccacg accagcttca gccccagcat gaccgaagtc gcgatctcgg agttcatcat
     1741 caggtacgag ccgtcgccca cggtgacgac gacctcgcgc tccggctgcg cgagcttcac
     1801 gcccaggccg cccgcgatct cgtagcccat gcaggagtag ccatactcca cgtggtatcc
     1861 gccgggcacc gacgtgcgcc agagcttgtg cagctccgcg ggcagcgtgc cggcggcgca
     1921 gacgacgatg tcgttcgcgg gcgagtcggc agccgagtca cggatcgccc cgatcacgtc
     1981 ggcgtcgtag ggcagggcgt cggcgcgcgg cgcggtcgtc agctcggtga cgcgcgcgtt
     2041 ccacgcgctc gcggc
//
LOCUS       DASVLN010000313         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_177764, whole genome shotgun sequence.
ACCESSION   DASVLN010000313 DASVLN010000000
VERSION     DASVLN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177764"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1132)
                     /locus_tag="VF453_07575"
     CDS             complement(68..1132)
                     /locus_tag="VF453_07575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7687549.1"
                     /translation="MTTHSPTPSPALDDRPRTAVIMQLNGMGDLVWHAQYFRRIAETS
                     RDGQVTLIAPPTVMARDLLGHEPWLREVIDFDRRPRRSERRRGRHSGLLGPLRFGAEL
                     VPKKLERMVLFSDHPGRGVIACWRAGIPVRIGYGATWLQRRMLTKSPWITMYDGPAVA
                     GYKDATTFAIAQGWCDAPIVPRISVRPDALERMRARLAPLPRPLHAIAIGASEPYKQW
                     GEDNFVALCGLLAARGQGVVMLAGPAERPLAQAILARIAPPLRARVDAITDGTVADSV
                     AALSLAQTCIGNDTGAANIAAAVGTRTWVILGPRPPLEHDPAHLAMIQAPTLAEIAPA
                     EVVRHVAEAGLLEPCTQAPA"
     gene            complement(1224..2198)
                     /locus_tag="VF453_07580"
     CDS             complement(1224..2198)
                     /locus_tag="VF453_07580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7687550.1"
                     /translation="MLHQWVGMGDLVWHIPYFREIARTSRDGQVSVIASPTCFARELL
                     GHEPWAREVIDFDRHPRRHEERAGRHAGVPGLWRFGRELRGHGFDRIVLFTNHTNRAV
                     VAAVAGIPERLGYGTSWLQRRLLSHGPWIRRYRGPAVTAYKDATAFAIAHGFAAAPIV
                     PTLVVRPEALARVQARVAGLPRPLHALSIGSSEPYKQWGTDNFAALASEIARAGHGVA
                     LVGGPAEREMAQAILGRLEPALRPRVLAMTDGTVGDTVAAMSLARTCIGNDTGACNIA
                     AAVGTPTWVVLGPRPPLEHDPRTLRMIAAPSLDEIRPADVAPHALSAA"
     gene            complement(2249..>2433)
                     /locus_tag="VF453_07585"
     CDS             complement(2249..>2433)
                     /locus_tag="VF453_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687551.1"
                     /translation="AVGPLAERSLADVRAAVTRAAKPLQRYRCAACGFEAQRHFWQCP
                     GCLSWDSYPPLRIEEQ"
BASE COUNT          298 a          873 c          966 g          296 t
ORIGIN      
        1 cgcatcagca cgccggtgcg cggggggggc ccgtcggccg cgtggcggtg gtgcggcagg
       61 gcgcgcgtca tgccggcgcc tgcgtgcacg gctcgagcag tccggcctcg gcgacgtgcc
      121 gcacgacttc cgcgggcgcg atctcggcga gggtgggtgc ctggatcatc gccaggtgcg
      181 cggggtcgtg ctccagcggc gggcgcgggc cgaggatcac ccaggtgcgc gtgcccaccg
      241 cggcggcgat gttggcggcg ccggtgtcgt tgccgatgca ggtctgcgcg agcgacagcg
      301 cggcgacgct gtcggccacg gtgccgtccg tgatcgcgtc gacgcgggcg cgcagcggcg
      361 gcgcgatccg cgcgaggatg gcctgcgcca gcggtcgttc ggccgggccg gccagcatca
      421 cgacgccctg gccgcgcgcg gcgagcagcc cgcacagcgc gacgaagttg tcctcacccc
      481 attgcttgta cggctccgac gcgccgatcg cgatggcgtg cagcggccgc ggcagcggcg
      541 cgaggcgggc gcgcatgcgc tccagcgcgt cgggccgcac cgagatccgc ggcacgatgg
      601 gcgcgtcgca ccagccctgg gcgatggcga aggtcgtcgc gtccttgtag ccggcgacgg
      661 ccgggccgtc gtacatcgtg atccacggcg acttcgtgag catgcgccgc tgcagccagg
      721 tcgcgccgta gccgatgcgc accgggatcc ccgcgcgcca gcacgcgatc acgccgcggc
      781 ccgggtggtc ggagaacagc accatgcgct cgagcttctt cggcacgagc tcggccccga
      841 agcgcagcgg cccgagcagg ccgctgtggc ggccccggcg gcgctcgctg cggcgcgggc
      901 ggcggtcgaa gtcgatcacc tcgcgcagcc agggctcgtg gccgagcagg tcgcgcgcca
      961 tcaccgtcgg cggcgcgatc agcgtgacct ggccgtcgcg gctggtctcg gcgatgcggc
     1021 ggaagtactg ggcgtgccag acgaggtcgc ccatgccatt gagctgcatg atcacggcgg
     1081 tgcgcgggcg gtcgtcgagg gcgggcgagg gcgtgggcga gtgcgtggtc aaggtcgtgc
     1141 gagtggcgat gccggcgtca ccggcaagtg tggagggacg gaggcaggcg cggtagcggg
     1201 ccgacccgcg caccgccacg gcttcaggcc gccgacagcg cgtgcggcgc gacgtccgcg
     1261 ggccggatct cgtccaggct cggcgcggcg atcatgcgca gcgtgcgggg atcgtgctcc
     1321 agcggcggcc gcggcccgag gacgacccag gtcggcgtgc ccaccgcggc ggcgatgttg
     1381 caggcgccgg tgtcgttgcc gatgcaggta cgggcgagcg acatcgccgc caccgtgtcg
     1441 cccacggtgc cgtcggtcat cgccagcacg cgcggccgca gcgccggctc caggcgcccc
     1501 aggatcgcct gcgccatctc gcgctccgcc ggcccgccca ccagggccac gccgtggccg
     1561 gcgcgcgcga tctcgctcgc gagcgcggcg aagttgtccg tgccccactg cttgtacggc
     1621 tccgacgagc cgatcgacag cgcgtgcagc ggccgcggca gccccgcgac gcgcgcctgc
     1681 acgcgcgcga gcgcctccgg ccgcaccacc agcgtcggca cgatgggcgc ggccgcgaag
     1741 ccatgggcga tcgcgaaggc ggtggcgtcc ttgtacgcgg tcacggcggg gccccggtag
     1801 cgccggatcc atggcccgtg cgacagcagc cggcgctgca gccagctggt gccatagccc
     1861 aggcgctccg ggatgccggc gaccgcggcc accaccgcgc ggttcgtgtg gttggtgaac
     1921 agcacgatgc ggtcgaagcc gtgcccgcgc agctcgcggc cgaagcgcca gaggccgggc
     1981 acgcccgcgt gccggcccgc gcgctcctcg tggcggcggg gatggcggtc gaagtcgatc
     2041 acctcgcgcg cccagggctc gtggccgaga agttcgcgcg cgaagcaggt cggcgaggcg
     2101 atgaccgaga cctggccgtc gcggctcgtc cgcgcgattt cgcggaagta ggggatgtgc
     2161 cagacgaggt cgcccatgcc gacccattgg tggagcacgg cggtacgcgg gcgatcatcg
     2221 gccggacccc tggcgggcgt cgggctcgtc attgttcctc gatgcgcagc ggcggatagc
     2281 tgtcccagct caggcagccc gggcactgcc agaagtggcg ctgcgcctcg aagccgcagg
     2341 ccgcgcagcg gtagcgctgc agcggcttgg ccgcgcgcgt cacggcggcg cggacgtcgg
     2401 cgagcgagcg ctcggccagc ggcccgaccg ccc
//
LOCUS       DASVLN010000314         3084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_285248, whole genome shotgun sequence.
ACCESSION   DASVLN010000314 DASVLN010000000
VERSION     DASVLN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285248"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..503)
                     /locus_tag="VF453_07590"
     CDS             complement(33..503)
                     /locus_tag="VF453_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX7687552.1"
                     /translation="MGEASGLPARPSSLNDKERHMYGFKTTITAPSFDAAVEQVTQAL
                     KAEGFGVLSDIDVQRALRDKLGAEMRPYRILGACNPPLAHQAVTAEPDIGLLLPCNVV
                     VREEAPGRHVVGFLDPQTMVGLVGRNDVKPVADEAGERLRRACAALGGTPVIGV"
     gene            complement(520..1800)
                     /locus_tag="VF453_07595"
     CDS             complement(520..1800)
                     /locus_tag="VF453_07595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX7687553.1"
                     /translation="MAHIVILGAGVGGMPAAYELRDRLGQEHKITVVNAVDYFQFVPS
                     NPWLAVGWRDRQDITFPIRPALERRGIDFIAQPCRRIDAARNAVEVEDGTRVSYDYLV
                     IATGPRLAFEEVPGSGPQGGFTQSICTVDHAEQAWNRYRAFLEEPGPAVIGAMPGASC
                     FGPAYEFAFIFSRDLQKRKLRHRVPITFVTSEPYIGHLGLGGVGDSKSMLESELRNHD
                     VKWITNARVTKVDDGTMFVTESDDAGQPRREHQLPFRYGMMLPAFKGVDPVAGVEGLC
                     NPRGFVLIDEFQRSKAYRNIFAAGVCVAIPPVEATPVPTGAPKTGYMIETMVTAIVHN
                     IAADIAGQAATARASWNAICLADMGDTGAAFVALPQIPPRNVNWFKKGKWVHYAKVAF
                     EKYFIRKMKSGSSEPVYEKYVLKALGITRLREHA"
     gene            complement(1826..>3084)
                     /locus_tag="VF453_07600"
     CDS             complement(1826..>3084)
                     /locus_tag="VF453_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7687554.1"
                     /translation="ARAAHQAGQARDAQAGALWRPTIALEGSVAKAASETSTTGARFS
                     APGLGQSDGVAFDTSVTSGTRTGYALGLRQPVYSRERSAQSQRLHVAAQVAEAQWQLA
                     GQDLVLRSAQAYFDAALAAEQLRLAIQQQAVVDKALVEARDRFRIGDRPITDVHEATA
                     RAEALKAQRLAVETELEIKTQELADLTGLTLDAKALALPGRTPPVDDLADLATWIGRS
                     AQHSPMLALAQARVDAARAQADADRAAFSPTVDIVAQIGREHLDGRGDFGAAGNTASN
                     RAIGIQVSVPLYTGGLRSARATETQALADEARAGEELAQQQAAQQTRAAWLDLAVGRS
                     QSAALEASLEASRARLDATRTGVQAGDRTTLDLLNAENDAAAAELNLQQARVRLLLDR
                     LRLAATAGELDDSLLQRLNAQWLPHP"
BASE COUNT          384 a         1077 c         1133 g          490 t
ORIGIN      
        1 cgggccgatc cgccggccgc cggcccgccg cgtcacacgc cgatgaccgg cgtgccgccc
       61 agcgcggcgc aggcgcggcg aaggcgctcg ccggcctcgt cggccacggg cttgacgtcg
      121 tttcgtccga cgaggccgac catggtctgc gggtcgagga agcccaccac gtggcgtccc
      181 ggtgcctcct cccgcacgac gacgttgcag ggcagcagca ggccgatgtc gggctcggcc
      241 gtcacggcct ggtgcgccag cggcggattg caggcgccga ggatgcggta gggccgcatc
      301 tcggcgccga gcttgtcgcg cagcgcgcgc tgcacgtcga tgtcgctcag cacgccgaag
      361 ccctcggcct tcagggcttg cgtcacctgc tcgacggccg cgtcgaagct cggggcggtg
      421 atcgtggtct tgaatccgta catgtggcgc tccttgtcgt tgagggagga cggtcgcgcg
      481 ggcagcccgg aggcttcgcc cacgggaagc ccgcggggct caggcgtgct cgcgcaagcg
      541 cgtgatgccc agggccttga gcacgtattt ctcgtagacc ggttcggacg agccgctctt
      601 catcttgcgg atgaagtact tctcgaaggc caccttcgcg tagtggaccc acttgccctt
      661 cttgaaccag ttgacgttgc gcggcgggat ctgcggcagt gcgacgaagg ccgcgccggt
      721 gtcgcccatg tccgcgaggc agatcgcgtt ccagctcgcg cgggcggtcg ctgcctgccc
      781 ggcgatgtcg gcggcgatgt tgtgcacgat cgccgtgacc atggtctcga tcatgtagcc
      841 ggtcttgggc gcgcccgtgg gcaccggcgt ggcctcgacc ggcgggatgg ccacgcacac
      901 gccggccgcg aagatgttgc ggtaggcctt gctgcgctgg aactcgtcga tcagcacgaa
      961 gccgcgcggg ttgcacaggc cctccacgcc ggccaccgga tccacgccct tgaaggccgg
     1021 cagcatcatc ccgtacctga acgggagctg gtgctccctc ctcggctggc cggcgtcgtc
     1081 cgactcggtg acgaacatgg tgccgtcgtc caccttcgtc accctggcgt tggtgatcca
     1141 cttgacgtca tggttgcgca gctcgctctc cagcatgctc ttggagtcgc cgacgccacc
     1201 caggccgagg tggccgatgt acggctcgga cgtcacgaaa gtgatgggca cccggtggcg
     1261 cagcttgcgc ttctgcaggt cacggctgaa gatgaaggcg aactcgtagg cggggccgaa
     1321 gcaggaggcg ccgggcatcg cgccgatgac cgccggcccg ggctcctcga ggaatgcccg
     1381 gtaccggttc caggcctgct cggcatggtc caccgtgcag atcgactgcg tgaagccgcc
     1441 ctgcgggccc gatccgggga cttcctcgaa ggccagccgc ggccccgtcg cgatcaccag
     1501 gtagtcgtag gaaacacgcg tgccgtcctc cacctcgacc gcgttgcggg ccgcatcgat
     1561 gcgcctgcag ggctgggcga tgaagtcgat gccgcggcgc tcgagcgcgg gccggatcgg
     1621 gaacgtgatg tcctggcggt ctcgccagcc cacggcgagc caggggttgg acgggacgaa
     1681 ctggaagtag tcgaccgcgt tgacgacggt gatcttgtgt tcctgcccga gccggtcgcg
     1741 caactcgtaa gctgcgggca tgccgccgac gccggcaccc aggatgacga tgtgcgccat
     1801 ggttgactcc tcgaatcgcg cgtgttcagg gatgcggcag ccactgcgcg ttcagtcgct
     1861 gcaacaggga atcgtcgagt tcgccggccg tggcggccag gcgcaggcgg tcgagcagca
     1921 ggcgcacgcg ggcctgctgg aggttcagtt cggcggcggc cgcgtcgttc tcggcattga
     1981 gcaggtccag ggtcgtgcgg tcgccggcct gcacgccggt gcgcgtggcg tcgaggcggg
     2041 cgcggctggc ctcgagcgac gcctccagcg ccgcgctctg gctgcgcccc acggccaggt
     2101 ccagccatgc cgcgcgcgtc tgctgcgcgg cctgctgctg cgccaattcc tcgcccgccc
     2161 gggcctcgtc ggcgagcgcc tgcgtctcgg tggcgcgcgc gctgcgcagg ccgccggtat
     2221 acagcggcac gctgacctgg atgccgatcg cacggttgct cgccgtgttg cccgcggcgc
     2281 cgaagtcgcc gcggccgtcg aggtgctcgc ggccgatctg cgcgacgatg tccacggtgg
     2341 gcgagaacgc ggcgcggtcg gcgtcggcct gggcccgcgc cgcgtcgacg cgtgcctggg
     2401 ccagcgcgag catcgggctg tgctgcgcgc tgcgcccgat ccaggtggcg agatcggcca
     2461 ggtcgtcgac cggtggcgtg cggccgggca gcgcgagggc cttcgcatcg agcgtcaggc
     2521 ccgtcaggtc ggcgagctcc tgcgtcttga tctccagctc ggtctccacc gccaggcgtt
     2581 gcgccttcag cgcctcggcg cgcgcggtgg cttcgtggac gtcggtgatc ggccggtcgc
     2641 cgatacggaa gcggtcccgc gcttcgacca gggccttgtc gaccaccgcc tgctgctgta
     2701 tcgccagccg cagctgctcc gcggcgagcg cggcgtcgaa gtaggcctgc gcgctgcgca
     2761 gcaccaggtc ctgcccggcg agttgccatt gcgcttcggc cacctgggcc gccacgtgca
     2821 gccgctgaga ctgcgcgctg cgctcgcggc tgtagaccgg ctgccgcagg cccagcgcat
     2881 agcccgtccg ggtgccgctg gtcaccgacg tgtcgaaggc cacgccgtcg ctctggccga
     2941 ggccgggtgc cgagaagcgc gcaccggtgg tggaggtttc gctggcggcc ttggccacgc
     3001 tgccctcgag cgcgatcgtc ggccgccaca acgcaccggc ctgggcgtcc cgggcctggc
     3061 cggcctggtg cgcggcgcgg gcgg
//
LOCUS       DASVLN010000315         1817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_16111, whole genome shotgun sequence.
ACCESSION   DASVLN010000315 DASVLN010000000
VERSION     DASVLN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16111"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VF453_07605"
     CDS             <1..538
                     /locus_tag="VF453_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7687555.1"
                     /translation="GTAAIQVARAAGARVIAAASTDEKCALCRRLGADEAINYAATPL
                     KDALKALTAGRGVDVVFDPVGGAYAEPALRAIAWRGRYLVIGFADGQIPALPWNLPLL
                     KGASIVGVFWGEFVRREPEANARMLGTLASQYAHGLLKPVIDEVLPLSRLPEAYARLA
                     GRRVLGKLVLVPDDLMPA"
     gene            743..1525
                     /locus_tag="VF453_07610"
     CDS             743..1525
                     /locus_tag="VF453_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7687556.1"
                     /translation="MAVKVLILEDNPAARDFLGRVVRESFSDTITIVEAGDLESARRA
                     VRIAGGAQGYHGVDPFKLILVDLELPDGSGLELLEELRQYPATKIVTTLYSDDEHLFP
                     ALQSGADGYLLKEDRFELLVEELQKIVRGEPPLSPAIARRLLGHFRINQRTGMNRRIT
                     DEGNGGAMRTAIAGLSTALAFTAPAPVPVEIEPPLDLPALTPREVEVLTFLSKGFTIK
                     EIAGLMSIKWFTVNDHIKSIYRKLNVSSRAEAAVLASKQGLV"
     gene            1625..>1817
                     /locus_tag="VF453_07615"
     CDS             1625..>1817
                     /locus_tag="VF453_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687557.1"
                     /translation="MRRGTIRPMGWRRLLLLPLALLASLAVVLLARTLAQAPDLDARW
                     RLDGSGHLQLIASDAPALRA"
BASE COUNT          255 a          674 c          642 g          246 t
ORIGIN      
        1 cggcaccgcg gcgatccagg tcgccagggc ggccggcgcg cgcgtgatcg cggcggcctc
       61 gaccgacgag aagtgcgcgc tgtgccgcag gctgggcgcg gacgaggcga tcaactacgc
      121 cgccacgccg ttgaaggatg cgctcaaggc gctgacggcg gggcgcggcg tcgacgtcgt
      181 cttcgacccg gtcggcggcg cctacgccga gcccgcgctg cgcgcgatcg cctggcgcgg
      241 gcgctacctc gtgatcggct tcgccgacgg ccagatcccg gcgctgccgt ggaacctgcc
      301 gctgctcaag ggcgcgtcca tcgtcggcgt gttctggggc gagttcgtgc gccgcgagcc
      361 cgaggccaac gcgcgcatgc tgggcacgct cgcgtcgcag tacgcgcatg gcctcctcaa
      421 gccggtgatc gacgaggtgc tgccgctgtc gcgcctgccc gaggcctacg cccgcctggc
      481 cggacggcgg gtgctgggca agctggtgct cgtgcccgac gacctgatgc cggcctgacg
      541 gcccatttgc gtgcacttcc gcacgtaccc gctttccgga ccgcgcgaca ggcttgaccc
      601 gtcccccgaa acgggggatg cggttacacg cccgtctcgc taggatgcct cccagtgccg
      661 agccccatgt cggcacaccg cgcgccagaa ggagcctcca gcctcccgcg cgaccgacac
      721 caggaaggca aaggggaacg gcatggcagt caaggttctg atcctcgagg acaaccccgc
      781 ggcgcgggat ttcctcggac gcgtggtgcg cgagagcttc agcgacacca tcaccatcgt
      841 cgaggcgggc gacctcgaga gcgcgcgccg ggccgtgcgc atcgccggcg gcgcgcaggg
      901 ctaccacggc gtcgacccgt tcaagctgat cctggtcgac ctggagctgc cggacggctc
      961 ggggctcgag ctgctcgagg agttgcgcca gtaccccgcc accaagatcg tcaccacgct
     1021 gtactcggac gacgagcacc tgttcccggc gctgcagtcc ggcgccgacg gctacctgct
     1081 caaggaggac cgcttcgagc tgctggtcga ggagctgcag aagatcgtcc gcggggagcc
     1141 gccgctgtcg ccggcgatcg cgcgccggct gctgggccac ttccgcatca accagcgcac
     1201 cggcatgaac cgccgcatca ccgacgaggg caacggcggc gcgatgcgca ccgccatcgc
     1261 cggcctgagc accgcgcttg cgttcaccgc gccggcgccg gtgccggtgg agatcgagcc
     1321 gccgctggac ctgccggcgc tgacgccgcg cgaggtcgag gtgctgacct tcctcagcaa
     1381 gggcttcacg atcaaggaga tcgcggggct catgtcgatc aagtggttca cggtgaacga
     1441 ccacatcaag tcgatctacc gcaagctcaa cgtctccagc cgcgcggagg ccgcggtgct
     1501 cgcgagcaag caggggctcg tctgatcatg cgccctgcgc acgcgcgcag cgccgcggcc
     1561 ggccgggcct ccgcagccgc ccccacctga cgtgcgcgcg cgcacgccgt ccggaacgac
     1621 cgcgatgagg cgcggcacca tcaggcccat gggttggcgg cggctgctgc tgctgccgct
     1681 ggcgctgctc gcgtcgctcg cggtggtgct gctcgcgcgg acgctcgcgc aggcgcccga
     1741 cctcgacgcc cgctggcgcc tggacggcag cggccacctg cagctgatcg cctccgacgc
     1801 gcccgcgctg cgcgccg
//
LOCUS       DASVLN010000316        15020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_230623, whole genome shotgun sequence.
ACCESSION   DASVLN010000316 DASVLN010000000
VERSION     DASVLN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15020
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230623"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..421)
                     /locus_tag="VF453_07620"
     CDS             complement(89..421)
                     /locus_tag="VF453_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687558.1"
                     /translation="MIVDIPGMRLGRGQNDRENHWARARRVKAEHHAVGWSLAGRPKP
                     ALPCVIRITRAAPGNGLDTDNLQGACKAVRDAIAKWIGVDDREVDVVRYEYAQARALW
                     GVRIEVVR"
     gene            complement(447..839)
                     /locus_tag="VF453_07625"
     CDS             complement(447..839)
                     /locus_tag="VF453_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HEX7687559.1"
                     /translation="MERTFRLVHPIARANAIEAIRQAPDGYAVTIKPRRRSLDQNAKF
                     HAICSDLERSGLAFRGKARTSAQWKAILISGHAIATQQECEVVEGIEGEVINLRESSA
                     AMSVQRGSSLIEYAIAFCAMNGIGGVNA"
     gene            complement(842..1318)
                     /locus_tag="VF453_07630"
     CDS             complement(842..1318)
                     /locus_tag="VF453_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687560.1"
                     /translation="MTKKSYVPKTGSHPRRAIEILEGYGERWIASTQLAELLCVSPFA
                     VRSLLEVAVRHEYIELKHEAHRNFYRLGPRRMGAQDAASAHSIDEAPRAGRRPPSVFD
                     MRDENIPMPTGAQFGVWADGRINISRAGEITEQLSPAEARALALVLRERAMEEEGA"
     gene            complement(1315..2184)
                     /locus_tag="VF453_07635"
     CDS             complement(1315..2184)
                     /locus_tag="VF453_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687561.1"
                     /translation="MRPPTPKPHAACSGQGRRSMREADFAPFAQHLADIWSLKGQVLN
                     GGGKALWFRALQAYSLDAIRAALDAYLRDPSTKGFLPMPSDIIGRLDRADGRPGAEEA
                     WATAVGCQDEAATVVWTEETSRAMGLCRPILLAGDEVGARMAFREAYERLVAEARSVR
                     RPVTWSVSLGMDPEGRRVAIEAAQAAGRLPVPAAGDAPLALPAPRGDVLTPLTQAAPA
                     EAKAALLRLRQSLTEPRAEPPSRDAQARERADQAKKSTQERVAAAFPGLHDAHVGARA
                     LNAARVQPRRKIA"
     gene            complement(2120..3073)
                     /locus_tag="VF453_07640"
     CDS             complement(2120..3073)
                     /locus_tag="VF453_07640"
                     /inference="COORDINATES: protein motif:HMM:NF018785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HEX7687562.1"
                     /translation="MPLEVARLRRSKAWLICKRTPALAFYMLNLWTACWHERPAGSLE
                     DDDDVLADQAMCDPAKWDKVKADVLRGWVLCDDGRYYHPTVCEKVVEAWNAKLDQRWR
                     TECSRIKKHNDRHGTNVPRPTFEEWLSADCPQGQRLPVTRDNDGTGGGQVGETRSKGQ
                     GEGQGEREGQGQGQGESMLGGAARTDAAADASAQPRTTRAPRGERLPKGWTLPREWGV
                     WAIETYPLWTPEKVRLEAERFADHWRAKTGKDATKLDWLATWRNWCRSPIAHRDDPKP
                     AGAPPARGRGSPSDADLAAANAAADAEAARRLFGAGETIDA"
     gene            complement(3121..6624)
                     /locus_tag="VF453_07645"
     CDS             complement(3121..6624)
                     /locus_tag="VF453_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEX7687563.1"
                     /translation="MTDYQAFLREKIKLARFDGFQVDPAEINPALKPHTRDIVRWGCL
                     GGRRAIFASFGLHKTATQIELMRLAGVHRPGLRLITIPLGVRQEFFREVSERFKGEYA
                     VDLRFIRQDSEIGDERTIYLTNYESIREGKLSPRLFRASSLDEASILRSFGSKTYQEF
                     LPLFEQVELKYVATATPSPNRYKELIHYAGYLGVMDTGQALTRFFQRDSEKAGNLTLY
                     PHKTQEFWLWVASWAVFIQYPSDLGHSDEGYVLPEMDVRYHEVPSDYAKAGHEKDGQA
                     LMFTDPALGLSAAATEKKDSLPARVAKVAEILAADPADHFVVWHDLEDERHALQEAIP
                     EAVSVWGSQQLDERENRIVGFGDGAFRVLSTKPVIAGSGCNFQRHCHREVFAGIGFKF
                     NDFIQAIHRVHRFGQASRVRIDIVYSEAEREVLRTLQAKWKQHEEMVATMTEIIKKFG
                     LDQLAMQETLARTLGVERVEVRGDRFVVANNDCVLEAQGLPENHVDLIVTSIPFANHY
                     EYSARYEDFGHTEDNDHFWAQMDHLTPELLRILKPGRLYCCHVKDRILFGNVTGAGAP
                     TVSPFHAEAIFHGKKHGFDYMGLITVVTDVVRENNQTYRLGWSEQCKDGTKMGVGSPE
                     YIVLFRKPQTDRSKGYADMPVSKSKGDYTRAHWQVDAHAFWRSSGNRQLTAEELAALG
                     PDKLAKMFTAYSLANVYDYEFHVKIGEELEARGALPSTFMSLAPGSHDPGVWHDVTRM
                     LTLNSEQARRAVEKHVCLARGSLVLTKERGYVPIETVQPGEHALTHRGRWRRVLVARQ
                     TGIKPTITVRAQGVPGLTLTPDHKLWARVTDWVRERDGAERAEPQWVEAQHTLGAYVN
                     LKLPETGDNEVADHIWWIAGRWLADGHYGARGEIVITCGFHEVEGLCASLGERAGTVN
                     RTGTAAQIRVRDRDGAIRATLDGCGEGAAGKRMPPAAMELGHSAAHALLTGYLSGDGH
                     FLADRGRWMATTVSKALALGVAALAQRVFGSIASIHQGRPEREVEIQGRRVQCRQEWV
                     VSFDTVASGRKKPFVRDDGAWKKVRSLEDSGDAEVWCLRVEEDESFTAEGCIVKNCPL
                     QFDIVDRLIERYSNPDELVYDPFCGLGTVPYRAILKGRRGGGSELNPAYFFDQVHYLR
                     SAEREFSMPSLFDTLDGEQAA"
     gene            6682..6891
                     /locus_tag="VF453_07650"
     CDS             6682..6891
                     /locus_tag="VF453_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687564.1"
                     /translation="MKSKERISVNSSEILQAAATIVSGRVSAHFAAGKRPEDLDAGLW
                     LIEAMYLIEGVVHEFEENRLYKNPV"
     gene            complement(6899..8356)
                     /gene="dcm"
                     /locus_tag="VF453_07655"
     CDS             complement(6899..8356)
                     /gene="dcm"
                     /locus_tag="VF453_07655"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEX7687565.1"
                     /translation="MKFLSVCSGIEAASVAWRPLGWKAWAFSEIDRFPAAVLAHHYPE
                     TPNFGDMTGFKDWPDATLDLLCGGTPCQSFSNAGLRAGLDDPRGQLMLTFGAIAARYR
                     PRWLVWENVPGVLSSNGGRDFGAFLGLLGFLGYGFAYRVLDAQFVRVDGHGRAVPQRR
                     NRVFVVGCLGDWRSAAAVLLERESLRGDPAPRRSAGARVAATLTRGAESGGRGSYASR
                     RQEDDINIVAGALAGTGPGAGWRIGADEAAANHCIAIQGSMIGRADDAGPAASGLDLT
                     GASFTLTKQDVHAVAVAATITAQIGEQTDMDARNGNLVAHAFDARQRDVVQYGSIAGP
                     LDTDAFSQAIAFDPTQITSRENRSNAQPGDPCHTLSKGMHAPAIAFKAGAGSKAASIT
                     ASEHVTPTLGACPSGNNSVPSIASAMRVRRLTPTECERLMGFPDGYTAITWRGKPAAD
                     GPRYKALGNSWATNCARWVGRRIAIVDSIHPPEVA"
     gene            complement(8353..8592)
                     /locus_tag="VF453_07660"
     CDS             complement(8353..8592)
                     /locus_tag="VF453_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687566.1"
                     /translation="MTRIVFEEIAVKGTFRWRDASGKRHQETRKFAQTYNPFNTSAEG
                     LIKSRAQIYAEVKRERDLWLLRRENDQRDIDRERA"
     gene            complement(8589..8966)
                     /locus_tag="VF453_07665"
     CDS             complement(8589..8966)
                     /locus_tag="VF453_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687567.1"
                     /translation="MEQQLSFPTGVMQATAPARREARTRRDLAIQQVGEAADRRNADW
                     CNKACEALRQFARKQAGVWTIELARAQFEKNLPPPSDLRAWGRVTQMAIRKGYIERVP
                     GQFFAAASSNNAAKSVWKKGPKA"
     gene            complement(9040..9411)
                     /locus_tag="VF453_07670"
     CDS             complement(9040..9411)
                     /locus_tag="VF453_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687568.1"
                     /translation="MTQAAFPVDVSPAEVMRKRNLGEAIALCLELAGLEPKQVVVEGK
                     HVDKAQWSRWVSGDEGIKWPKLRDLMDQCGNDAPLLWMVHARHYDLASLRQVENELER
                     QNRELREENAALRRAMRSALG"
     gene            complement(9714..9947)
                     /locus_tag="VF453_07675"
     CDS             complement(9714..9947)
                     /locus_tag="VF453_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7687569.1"
                     /translation="MKSIRLQLGMTQAELAARLGVTQGVVSHFEVGRRVLNPSMAAKL
                     IRAARGRGVQVTFDTLYGHLLRRTPPARAEPRA"
     gene            10041..10775
                     /locus_tag="VF453_07680"
     CDS             10041..10775
                     /locus_tag="VF453_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7687570.1"
                     /translation="MTESNSYRKKVTAEQREESARLKAIWATKWKGKRTQEELAEVLG
                     VGQAAVSHFLNGHTAISRKAAPIFAEAFKCKVADFSPRLAAEMEAGGSALQPAREAEA
                     AFDDSQVAADPGTITVGDAIAHLAATIRSMPETSRSRVGEELSLLASVPDSPTLIQRI
                     TRLLKPFDQPTPLAGVVATDERRESPGDLLERKLEEVPADRRSKMYALLEGMIDKVVY
                     ELRTQQATRAETMPVPEPNPEPASHP"
     gene            11440..11601
                     /locus_tag="VF453_07685"
     CDS             11440..11601
                     /locus_tag="VF453_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687571.1"
                     /translation="MSYQKSDDDWGTSFASTERPVPRLSAADRVLIAVGVVAVIGAIV
                     SIAYSGIGL"
     gene            11598..11846
                     /locus_tag="VF453_07690"
     CDS             11598..11846
                     /locus_tag="VF453_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687572.1"
                     /translation="MSALVVATRGPRVEIGKRWSPERVVRDDRAAGRFEMVNPMADGE
                     AGYIELTTQRALLEAVRDARTLARIERARTAQRVGAAA"
     gene            11843..12334
                     /locus_tag="VF453_07695"
     CDS             11843..12334
                     /locus_tag="VF453_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687573.1"
                     /translation="MNAHPPHDYRVPSNAELLAAAGAFEDRVLAAHLDAEDSAYARRQ
                     ALLDEINDVDRAAVFVALCQEGKVAVDEDRIDELLFEEVASRLNAEARAISLARNVHP
                     MRDQRRADRLVELLLAAEELVAAADASMDRGDLLQGAKSKLFGAWARCGGALVTQAHR
                     EAA"
     gene            12331..12618
                     /locus_tag="VF453_07700"
     CDS             12331..12618
                     /locus_tag="VF453_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687574.1"
                     /translation="MTNRVRLSDPLVRKLHGWELDHLRDLVEEQRVEIERLAAANEQL
                     QAEVYDLEGSADMWRDLAARDLADDESLGITRDGRLCVVTQPQQQQSGGLQ"
     gene            12615..13388
                     /locus_tag="VF453_07705"
     CDS             12615..13388
                     /locus_tag="VF453_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687575.1"
                     /translation="MNAVVDTPMSILSLADVTSRINAIQTVTRNKMIEGVHYGIIPGT
                     KKPTLYKAGSEMLLTMFGIGPRVRVEDLSNEHRLKYRVIVAGVHLATERAIGEGVGEC
                     SSNEERYQWREAICEAEFDSTPESMRRVKWARKNGRHYTINQIRTNPDDLGNTVLKMA
                     KKRAQIDMTLTALGVSDLFNQDIEDLPPELQPREDGDAPTTGGAGQAPATAGIEHPAL
                     KAAKTVQELAKAMSALGPDDKRKHVSYFNARMAELKGSK"
     gene            13385..14290
                     /locus_tag="VF453_07710"
     CDS             13385..14290
                     /locus_tag="VF453_07710"
                     /inference="COORDINATES: protein motif:HMM:NF021128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEX7687576.1"
                     /translation="MIEYRDPQGSPEWLAARRGVITASRAKDARDRSSGLDERQTAYV
                     RAILAGQSESEALAASGYKKAPTAEAVRACLDARRIEKKWSGAAISYAQDLARERCGG
                     VLPEQREGFAQRQGHEQEPFGVIAWIASTGLEVEEVGFVATDDRRFGTSLDRRVVGKN
                     AALEVKTMVSSVTLFRAIVDGDVSEYRDQCLFQLWMLSLDWIDLCLWCPDLNKLHIVR
                     IERDEDELQALEDDLVAFDALVTFYELALRRKLGIDDDLLNGEPVSVPDERPASASPP
                     IKSAAAPAAGPRASAPAPAIPELEF"
     gene            14294..>15020
                     /locus_tag="VF453_07715"
     CDS             14294..>15020
                     /locus_tag="VF453_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687577.1"
                     /translation="MTDEVLERHAAPTTLPAPAPEVAVAEKPLTIEQTIRVQFAALEP
                     TMTALAARHSNIAWDVTTPKGLSDAKAARHELRESGRFFLNRSLDAKKREVNDVKTIL
                     ESETARLVAIVKPVEDEINAIIVARENELAAEKAERERIAAERRARFEAEIAKITGMA
                     ARCAGISAERIANGIRLVEDMTFGEEWAEFAAAAAKAQAETLAAMRRLHAEALEAEAR
                     AAREAALEVERQELARQAEAQRQE"
BASE COUNT         2457 a         5107 c         4989 g         2467 t
ORIGIN      
        1 agctggccgc gcgacgccac cacggcccgc ggcgcggccg ggttcgcgcc ttcgtactgc
       61 cgcactcgcg gcggcggctt cttccagctc atcgcacgac ctcgatgcga acgccccaca
      121 gcgcgcgcgc ctgggcgtac tcgtagcgga cgacgtctac ctcccgatcg tcgacgccga
      181 tccacttcgc gatggcgtcg cgcaccgcct tgcaggcacc ctgcaggttg tcggtgtcca
      241 gcccgttgcc gggcgccgcg cgggtgatgc ggatgacgca gggcaacgcc ggcttcggcc
      301 ggccggcgag cgaccacccg accgcgtggt gctccgcctt cacccgccga gcacgcgccc
      361 aatggttttc gcggtcgttc tgcccacgtc cgagcctcat gcccgggatg tcgacgatca
      421 tgacggcgct cccgctagag gcaacgttag gcattgacac ctccaatgcc attcatcgca
      481 cagaaggcga tggcgtactc gatgaggctc gagccgcgct gcaccgacat cgccgcagag
      541 ctctcgcgaa ggttgatcac ttcgccctcg atgccctcga caacctcgca ctcctgctgc
      601 gtcgcgatgg cgtgcccgga gatcaggatc gccttccact gggcactcgt gcgggccttc
      661 ccgcggaagg cgaggccgga tcgctccagg tcgctgcaga tcgcgtggaa cttggcgttc
      721 tggtcgaggg agcggcgacg cggcttgatc gtcaccgcgt agccatctgg ggcttggcgg
      781 atggcctcga tcgcattggc gcgcgcgatc gggtggacga gtcgaaacgt gcgctccatg
      841 gtcaggcacc ctcctcttcc atcgcgcgct cgcgcagcac aagcgccagc gcgcgcgcct
      901 cggccggtga gagctgctcg gtgatctcgc cggcacgcga gatgttgatc ctgccgtcgg
      961 cccatacgcc gaactgcgca ccggtcggca tcggaatgtt ctcgtcgcgc atgtcgaaca
     1021 cgctcggcgg ccgccggccg gcgcgggggg cttcgtcgat ggagtgcgcg ctggccgcgt
     1081 cttgggcacc cattcggcga gggccgaggc ggtagaagtt gcggtgggcc tcgtgcttga
     1141 gctcgatgta ttcgtggcgc acggcgacct cgagcaggct tcgcaccgcg aacggcgaga
     1201 cgcacagcag ctcggccagc tgagtcgacg cgatccagcg ctcaccatag ccttcgagga
     1261 tctcgatcgc gcggcgcgga tggctgccgg tcttcggtac gtaggacttc ttggtcatgc
     1321 gatctttcga cgaggttgga ctcgggcggc gttgagcgcg cgcgcaccga catgagcgtc
     1381 atgcagcccc gggaaggccg cggcgacacg ctcctgcgtg gacttcttcg cctggtcggc
     1441 ccgttcgcgg gcctgcgcat cccggctcgg tggctccgca cgcggctccg tgagcgactg
     1501 gcgcaaccgc agcagcgccg ccttcgcctc cgccggcgca gcctgggtca gcggcgtcag
     1561 cacgtcgccg cgtggcgccg gcagcgcgag gggggcgtct ccggccgccg gcaccggcaa
     1621 ccgcccggcg gcctgtgcgg cctcgatcgc gacgcggcgt ccctccggat ccatgcccag
     1681 gctgaccgac caggtgacgg gccggcgcac cgaccgcgcc tcggcgacca ggcgctcgta
     1741 ggcctcgcgg aacgccatcc gtgcgccgac ctcgtcaccg gccagcagga tcggacggca
     1801 caggcccatc gcgcggctcg tctcctccgt ccagacgaca gtggcagcct cgtcctggca
     1861 gccgacggcg gtggcccacg cctcttcggc gcccgggcgg ccatccgcgc ggtcgaggcg
     1921 cccgatgatg tcgctgggca tgggcaggaa gcccttcgtg ctggggtcgc ggaggtaggc
     1981 gtccaacgca gcccggatcg cgtcgagcga gtaggcctgc agcgcgcgga accagagcgc
     2041 cttgccgccg ccgttcagca cttggccctt gaggctccag atgtccgcga ggtgctgggc
     2101 gaagggggcg aagtcggctt cacgcatcga tcgtctcccc tgccccgaac aggcggcgtg
     2161 cggcttcggc gtcggcggcc gcattcgccg cggcgagatc cgcgtcgctc ggggagccgc
     2221 ggccgcgcgc gggcggcgcg ccggccggct tgggatcgtc gcgatgcgcg atggggctgc
     2281 ggcaccagtt gcgccaggtc gcgagccagt cgagcttcgt cgcgtccttg ccggtcttcg
     2341 cgcgccagtg gtcggcgaag cgctcggcct cgaggcgcac cttctcgggc gtccacagag
     2401 ggtaggtctc gatcgcccag acgccccact cgcgaggcag cgtccagcct ttgggcagcc
     2461 gctcaccgcg tggagcacgg gttgtccgcg gctgggccga cgcgtcagcg gcggcgtcgg
     2521 tgcgcgcggc gccgcccaac atagattctc cctgtccctg tccctgtccc tctctctctc
     2581 cctgtccctc tccctgtccc ttggagcgcg tttccccaac ttgtccgcct ccagtcccat
     2641 cgttgtcccg agtgacaggc aggcgctgtc cctggggaca atctgcggac agccattcct
     2701 cgaaggtggg acgaggcacg ttcgtcccgt ggcggtcgtt gtgcttcttg atgcgcgagc
     2761 actcggtgcg ccagcgctga tcgagcttcg cgttccaggc ctcgacgacc ttctcgcaga
     2821 ccgtcgggtg gtagtagcgg ccgtcgtcgc agagcaccca gcctcgcagg acgtcggcct
     2881 tgaccttgtc ccatttggcc gggtcgcaca tggcctgatc ggcgagcacg tcgtcgtcgt
     2941 cctcgagcga gccggccggc cgctcgtgcc agcaggccgt ccagaggttg agcatgtaga
     3001 aggccagcgc cggcgtgcgc ttgcagatca gccaggcctt cgaccggcgc aggcgcgcca
     3061 cttccagcgg catgaacggg aagtccgtca gatcgcagtc gggcggcgtc aggggtgcag
     3121 tcacgcggcc tgctccccgt cgagcgtgtc gaacaggctc ggcatcgaga actcacgctc
     3181 ggccgaccgc aggtagtgca cctggtcgaa gaagtaggcc ggattgagct ccgagccgcc
     3241 gccgcggcgc cccttcagga ttgcgcggta cggcacggtg cccaggccgc agaacgggtc
     3301 gtagacgagc tcgtcggggt tgctgtaccg ctcgatgagg cgatcgacga tatcgaactg
     3361 cagcgggcag ttcttgacga tgcagccttc cgccgtgaag ctctcgtcct cttcgacgcg
     3421 caggcaccag acctcagcat cgccggaatc ttcgagcgaa cgcaccttct tccaggcacc
     3481 gtcatcacgc acgaaaggct tcttccggcc agacgccacg gtatcgaaag agactaccca
     3541 ctcctggcgg cactgcacgc gacgaccctg gatctcaact tcgcgctctg gccgaccttg
     3601 gtggatgctc gcgatcgatc cgaaaacacg ttgagccagc gcagcgacgc caagcgcaag
     3661 ggccttgctg acagtggtcg ccatccagcg gccgcgatca gcaaggaagt gtccatctcc
     3721 ggacaggtag cccgtcaata gtgcatgggc agccgaatgc cccagttcca tcgcagccgg
     3781 cggcatccgc tttccagcgg caccctctcc gcagccatca agagtggcac ggatcgcccc
     3841 gtcccggtca cgaactcgga tttgcgcggc cgtacctgta cggttgaccg tcccggcgcg
     3901 ctcgccaagg gaggcgcaga ggccctcgac ctcgtgaaag ccgcacgtga tgacgatctc
     3961 accgcgcgcg ccatagtgcc cgtcagcaag ccaacggccg gcaatccacc agatgtgatc
     4021 ggcgacttcg ttgtcgccgg tttcaggcag cttgaggttg acgtaggcgc ccagcgtgtg
     4081 ctgcgcctca acccattgcg gctcggcacg ctcggcaccg tcgcgctctc gcacccagtc
     4141 cgtcacgcga gcccagagct tgtgatcggg cgtcagcgtg agcccgggaa ccccttgtgc
     4201 gcgaaccgtg atggtcggct tgataccggt ctggcgcgcg accagcacgc ggcgccagcg
     4261 accgcgatgc gtcagggcat gctcgccggg ctgcaccgtc tcgatgggca cgtagccgcg
     4321 ctctttggtc agcaccaagc ttccacgggc caggcagacg tgcttctcga cggcccggcg
     4381 cgcctgctcg ctgttcagcg tcagcatgcg cgtgacgtcg tgccagacgc ccggatcatg
     4441 gctgcccggc gccaacgaca tgaaggtcga cggcaacgcg ccgcgggctt cgagctcctc
     4501 gccgatcttg acgtggaact cgtagtcgta gacgttggcc agcgagtagg ccgtgaacat
     4561 cttggccagc ttgtccgggc ccagcgcagc cagttcctcg gcggtgagct ggcggttgcc
     4621 gctcgagcgc cagaacgcgt gcgcgtcgac ctgccagtgc gcgcgcgtgt agtcgccctt
     4681 ggacttcgac acaggcatgt cggcgtagcc cttgctgcga tcggtctgcg gcttgcggaa
     4741 gaggacgatg tactcgggcg agccgacgcc catcttcgtg ccgtccttgc actgctcgct
     4801 ccagcccagg cggtaggtct ggttgttctc ccgcacgacg tcggtcacga cggtgatcag
     4861 gcccatgtag tcgaagccgt gcttcttccc gtggaagatc gcctcagcgt ggaacgggct
     4921 gaccgtgggt gcgccggcgc cggtgacgtt gccgaacagg atccggtcct tgacgtggca
     4981 gcagtacagc cggccgggct tgaggatccg cagcagctcg ggcgtcaggt gatccatctg
     5041 cgcccagaag tgatcgttgt cctcggtgtg gccgaagtcc tcgtaccggg ccgagtattc
     5101 gtagtggttg gcgaagggga tcgacgtcac gatcaggtcg acgtggttct ccggcaggcc
     5161 ctgcgcttcg agcacgcagt cgttgttcgc aacgacgaag cggtcgccgc gtacctccac
     5221 gcgctcgacg ccgagcgtgc gcgcgagcgt ctcctgcatc gcgagctggt cgagcccgaa
     5281 cttcttgatg atctcggtca tggtggccac catctcctcg tgttgcttcc acttcgcctg
     5341 cagggtccgg agcacctcgc gctccgcctc gctgtagacg atgtcgatcc gcacgcggct
     5401 cgcctgaccg aagcggtgga cgcggtggat cgcctggatg aagtcgttga acttgaagcc
     5461 gatgcctgcg aagacctcgc ggtggcaatg gcgttggaag ttgcagcccg agccggcgat
     5521 caccggcttt gtggacagga cgcggaacgc gccatcgccg aagccgacga tgcggttctc
     5581 gcgctcgtcg agctgctggc tgccccagac gctgaccgcc tcggggatcg cctcctgcag
     5641 cgcgtgacgc tcgtcttcga ggtcgtgcca gacgacgaag tggtcagcgg gatctgccgc
     5701 gaggatctcg gcgaccttgg cgacgcgcgc gggcaggctg tccttcttct ccgtcgcggc
     5761 ggccgacagg ccgagcgccg ggtcggtgaa catcagggcc tggccgtcct tctcgtgtcc
     5821 ggccttggcg tagtcgctgg gcacctcgtg gtaccggacg tccatctccg gcagcacgta
     5881 gccctcgtcg ctgtgcccga ggtcgctcgg gtactggatg aagaccgccc agctcgcgac
     5941 ccacaaccag aactcttggg tcttgtgcgg gtacagcgtc aggttgccgg ccttctccga
     6001 gtcgcgctgg aagaagcgcg ttagggcctg gcccgtgtcc atcacgccga ggtagcccgc
     6061 gtagtggatc agttccttgt agcggttcgg gctgggcgtc gcggtcgcca cgtacttgag
     6121 ctcgacctgc tcgaacagag ggaggaactc ctgataggtc ttcgagccga agctgcgcag
     6181 gatgctcgcc tcgtcgagcg acgacgcgcg gaacagccgc ggcgacagct tgccctcgcg
     6241 aatgctctcg tagttggtca ggtagatcgt gcgctcgtcg ccgatctcgc tgtcctggcg
     6301 gatgaagcgc aggtcgaccg cgtactcgcc cttgaagcgc tcggacacct cgcggaagaa
     6361 ctcctggcgg acgccgagcg ggatcgtgat caggcgcagg ccgggccggt gcacgccggc
     6421 gagtcgcatg agctcgatct gcgtggcggt cttgtgcagg ccgaaggacg cgaagatcgc
     6481 gcggcggccg cccaggcaac cccagcgcac gatgtcgcgg gtgtgcggct tcagcgccgg
     6541 gttgatctcg gccggatcca cctggaagcc gtcgaagcgg gcgagcttga tcttctcgcg
     6601 gaggaaggct tgatagtcgg tcatgctccg acctccggta cgatcattga tgccaccgat
     6661 gtccggtgga gggagaagtt tatgaagtcc aaagagcgca tttccgtcaa ctcgtcggag
     6721 atcctgcaag ccgccgcgac gatcgtctcg ggacgcgtct cagcccattt cgcggcggga
     6781 aagcgtccgg aagacctcga cgcgggcctc tggttgatcg aggccatgta cctcatcgaa
     6841 ggcgtcgtcc acgagttcga agagaaccgc ctgtacaaga atccggtctg aaaccgagtc
     6901 acgcgacctc cggcgggtgg atgctgtcga cgatggcgat ccggcgaccg acccagcgtg
     6961 cgcagttggt cgcccacgaa ttgccgaggg ccttgtagcg agggccatcg gcggccggct
     7021 tcccgcgcca ggtaatggct gtgtacccgt cggggaagcc catcaggcgc tcgcactccg
     7081 taggcgtcag ccgacgcacg cgcatggccg acgcgattga cggcacgctg ttgttgcccg
     7141 atggacacgc ccccaaggtc ggggtcacat gctcgctcgc tgtgatgctg gcggccttcg
     7201 accccgcgcc agccttgaat gcaatcgcgg gcgcgtgcat gcccttcgat agggtgtggc
     7261 acggatcgcc gggctgagca ttgctgcggt tctcgcggct ggtgatctgc gtcgggtcga
     7321 atgcgatggc ctgcgagaag gcatcggtat cgagcgggcc cgcgatcgaa ccgtactgca
     7381 cgacatcgcg ttggcgcgcg tcgaaggcgt gcgcgaccag attcccattc ctggcatcca
     7441 tgtccgtctg ctcaccgatc tgcgcggtga tcgtggcggc gacagcaacg gcatggacat
     7501 cttgcttcgt gagcgtgaag ctcgcgccgg tcaggtcaag cccgctcgcg gcgggcccag
     7561 cgtcgtcggc gcggccgatc atgctgccct ggatggcgat gcaatggttc gccgccgctt
     7621 catcggcgcc gatacgccag cctgcgccag ggccggttcc cgcaagtgca cccgcgacga
     7681 tgttgatgtc gtcttcttgt cgtcggctgg cgtagctacc tctgccaccg ctttcagcgc
     7741 cgcgagtaag tgtggcggca acgcgcgccc ccgcgcttcg gcgcggcgca ggatccccgc
     7801 gcaggctttc gcgctcaaga agtaccgcgg cggcacttcg ccaatctcca agacatccga
     7861 caacgaacac acggttccgt cgttggggga cggcgcgccc gtgaccgtcc actcgcacaa
     7921 actgagcgtc cagaacgcgg taggcgaacc catacccgag gaagcccagg agcccgagga
     7981 aggcaccaaa gtcccgtcct ccgttgcttg acaggacgcc ggggacgttc tcccatacca
     8041 gccagcgggg ccgatacctt gcagcaatgg caccaaaggt gagcatgagt tgaccacgcg
     8101 ggtcatccag tccagctcgg agtccggcgt tgctgaagga ctggcaaggg gttcctccac
     8161 agagaagatc gagagttgca tcgggccaat ccttgaagcc ggtcatatcg ccgaagttcg
     8221 gcgtctcggg gtagtgatgc gcgagcacgg cggcagggaa gcggtcgatc tcggagaagg
     8281 cccacgcctt ccagccgagc ggtcgccacg cgacggaagc ggcctcaatg cccgagcaga
     8341 cagaaaggaa cttcacgcgc gttcccgatc gatatcacgc tggtcgttct cgcgacggag
     8401 aagccaaagg tcgcgctcgc gcttaacctc tgcgtagatc tgcgcgcgcg acttgatcag
     8461 accttcggca gacgtgttga acgggttgta ggtctgcgcg aacttgcgcg tctcctggtg
     8521 ccttttgccg ctggcgtcac gccaacgaaa ggtgcctttg accgcgatct cctcaaagac
     8581 gattcgcgtc atgccttggg ccctttcttc caaaccgact tcgcggcatt gttcgagctg
     8641 gctgcagcga agaactgccc gggcacccgc tcgatgtagc ccttgcggat ggccatctgc
     8701 gtgacgcggc cccacgcgcg gaggtcgctg ggaggcggca ggttcttctc gaactgcgca
     8761 cgcgcgagct cgatcgtcca gacgccggcc tgcttgcggg cgaactggcg cagcgcctcg
     8821 caggccttgt tgcaccagtc ggcgttccgg cggtcggcgg cctcgccgac ctgctggatc
     8881 gcgaggtcgc gccgggtccg ggcttcacgg cgcgccggcg cggtggcctg catcacgccg
     8941 gtggggaagg aaagttgttg ttccatggtc agacggctcg gttcgtgaat ggatgctcgt
     9001 gcgcgacggc ccgagctcgg gtgtgcggcg accgctcgct catcccagcg cgctccgcat
     9061 ggcgcgccgc agcgcggcgt tctcctcgcg cagctccctg ttctgccgct cgagttcgtt
     9121 ctcgacttgg cgcaggctgg cgaggtcgta gtgcctcgcg tgaaccatcc acagcagcgg
     9181 cgcgtcgttg ccgcactggt ccatcaggtc gcgcaacttg ggccacttaa tgccctcgtc
     9241 gccactgacc cagcgcgacc actgggcctt gtcgacgtgc ttgccttcga cgacgacctg
     9301 cttgggctcc agtcccgcaa gctcaaggca cagggcgatc gcctcgccga ggttccgctt
     9361 gcgcatgacc tcggcagggc tgacatccac ggggaacgcg gcctgggtca cacaagcctc
     9421 cgcaagcttg ttgaggcgcg ttgagaggcg ctcgcgagga aaattttttc caggccatcg
     9481 agcggcgcac gcaccggcaa tctgccttgt gctcgcccgc tcggatcaac gacgtatcgg
     9541 ggatagttca tgtcttgcga gttcaacgtg gaggacgacg ccggtcgtcc cgggaatcag
     9601 gcgctgaacg cctgggcgcg cttgaaggtg cgcgacgttt ttgtgagagc gttcgctgcg
     9661 ctggcttcgg acttcgagct gttcgcgaag ctggaggaac ggaaaggaac ggctcaggcg
     9721 cgcggctcgg cgcgcgccgg cggcgttcga cgcaagaggt gcccgtagag ggtgtcgaac
     9781 gtgacctgga cgccgcgccc gcgcgcggcg cggatcaact tcgccgccat cgacggattc
     9841 agcacacgtc ggccaacctc gaagtggctg acaacacctt gggtgacgcc caggcgagca
     9901 gcgagctctg cctgtgtcat gcccagttga aggcggatcg atttcacggc ggaactcatg
     9961 actaatagta ccgccgggat ttttttacgt caataccgcc gggatttgaa aaataccggc
    10021 cagaggtcga ctatcagcgc atgaccgaga gcaacagcta taggaagaag gtgactgccg
    10081 agcagcgcga ggaatcggca cgtctcaagg cgatctgggc cacgaagtgg aagggcaagc
    10141 gaacgcagga ggaactcgcc gaagttctcg gcgtcggcca ggctgcggtg agccacttcc
    10201 tgaacggtca cacagcgatc tctcggaagg ctgcaccaat cttcgcggaa gccttcaagt
    10261 gcaaggtggc cgacttctct ccacgcctag cagccgaaat ggaggccggc ggaagcgcgc
    10321 tgcagcccgc acgggaggcc gaagcggcct tcgatgacag ccaagttgcg gccgaccccg
    10381 gaacgatcac tgttggcgat gcgatagcgc atctcgcggc aacgattcgc tcgatgcccg
    10441 agacctcccg atcgcgagtg ggcgaggaac tatcgctctt ggcctcggtg cccgacagtc
    10501 ccacgctgat ccagcggatc acgcggctgc tgaagccgtt cgatcaacca accccactcg
    10561 ccggcgttgt cgctacagac gagcgccgag aatcgccggg agatttgctg gagcgaaagc
    10621 tcgaggaagt ccctgcagat cgtcgcagca agatgtacgc tttgctggag ggcatgatcg
    10681 acaaggtggt ctacgagctt cgcacccagc aagctacgcg cgcggaaacc atgcccgtgc
    10741 ccgagcccaa tccagagccc gcctctcatc cctgaatacg ctgcgcagcg taccttcaag
    10801 cgcacgctgc caggcccatt cgcgccacgc cggctccgcc tcgggagagg ttatcagtgc
    10861 cagcggtggc gtgccctcgt ttgaccgcac gttggcggtc agagaatcca cccccactcc
    10921 gagcacgact tcacggcagt caaccacgac tgcgctcgca tgcgccagag cggcgtcatg
    10981 cacccagcac ttgaagtcca acacgttgga tctcgtcacg agcccgcgcg ctcgcacgac
    11041 cgctattccg tcggtaccaa gatccagctc cgccctgcac agggccgaca cgaagcggct
    11101 gccgccggcc gccccgtagc tggttgccgc caacgcggcc gagccgtctc gcggccgtgc
    11161 acctgactcc atgccggagt cggcgattcc atcgctcttc atcgagttct ccaccagggc
    11221 ggaagccgcc cgcgtacgac gctcggcaag ccgcctcgcg cccacaccct ccctaggttc
    11281 cctgtctttt attaccggaa cacttgacat catcactacc gccggtatta ttagcaacag
    11341 atcgacggta cttcttcatt cccgccggtc atacaggtga gcagtacgtc tttgctcccc
    11401 gattcgcaaa gccagtgtgc agaagcagga gatcatcgaa tgagctacca gaaatcggac
    11461 gacgactggg gaaccagctt cgcctccacc gaaaggccgg tgccgcgcct gagcgcggcc
    11521 gaccgagtcc tgatcgccgt cggcgtcgtc gcagtcatcg gcgccatcgt ttccatcgcc
    11581 tactcgggga tcggcctgtg agcgcgctcg tcgtcgccac ccgcgggccg cgcgtcgaga
    11641 tcggcaagcg ctggtcgccc gagcgtgtcg ttcgcgacga ccgcgcggcc gggcggttcg
    11701 agatggtcaa tccgatggcc gatggcgagg ccggctacat cgagctgacg acgcagcgcg
    11761 cgctgctcga agccgttcgc gatgcccgca cgctggcgag gatcgagcgg gcgcgcacgg
    11821 cgcagcgcgt gggagctgcg gcatgaacgc gcaccccccc cacgactacc gcgtcccgag
    11881 caacgccgag ttgctcgccg cggctggagc attcgaagat cgcgtgctcg cggcccacct
    11941 cgacgccgag gactctgcct atgctcgccg gcaagcgctg ctggacgaga tcaacgatgt
    12001 cgaccgtgcg gctgtcttcg tggcgctgtg ccaggaaggc aaggtggctg tcgacgaaga
    12061 tcgaatcgac gagctgctct tcgaagaggt tgcgtcgagg ctgaacgccg aggcgcgcgc
    12121 catctccctt gcccgcaacg tccatccgat gcgcgaccag cgtcgcgccg accgcctcgt
    12181 cgagctcctg ctcgctgcgg aggaacttgt cgctgctgca gacgcgagca tggatcgcgg
    12241 cgacctgctg cagggcgcga agtcgaagct gttcggcgcc tgggcccgct gcggcggcgc
    12301 cctggtgacg caggcgcatc gggaggccgc atgaccaatc gcgtccgcct ctccgatcca
    12361 ctcgtgcgca agctgcacgg gtgggagctg gaccacctgc gcgatctcgt cgaggagcag
    12421 cgcgtcgaga tcgagcgcct ggccgccgcc aacgaacaac tgcaggcgga ggtctacgac
    12481 ctcgaaggct ctgccgacat gtggcgcgat ctcgcggccc gcgacctcgc cgatgacgag
    12541 agcctcggca tcacccgcga tggacgcctc tgcgtcgtca cccagcccca acaacagcaa
    12601 tccggaggtc tgcaatgaat gccgtcgtcg acacgccgat gtcgatcctg tccctggcgg
    12661 acgtcacgag ccggatcaac gcgatccaga cggtgacccg caacaagatg atcgaaggcg
    12721 tccactacgg gatcatccct ggcaccaaga agccgacgct ctacaaggct ggcagcgaga
    12781 tgctgctgac gatgttcggc atcgggcccc gcgtccgggt cgaagacctg tcgaacgagc
    12841 accgcctgaa gtatcgcgtc atcgtggctg gcgtccacct ggcgacggag cgcgcgatcg
    12901 gcgaaggcgt cggcgagtgc tccagcaacg aagagcgcta ccagtggcgc gaagccatct
    12961 gcgaagcgga gttcgactcg acgcccgagt ccatgcgccg cgtgaagtgg gcccggaaga
    13021 acgggcgcca ctacacgatc aaccagatcc gcacgaaccc cgacgacctc gggaacaccg
    13081 tgctcaagat ggccaagaag cgcgcacaga tcgacatgac gctgaccgcg ctcggcgtct
    13141 ccgacctgtt caatcaggac atcgaggatc ttccgcccga actccagccg cgcgaggacg
    13201 gcgacgcccc gacgaccggc ggcgcgggac aggcgccggc gacggccggc atcgagcatc
    13261 ccgcgctgaa ggccgccaag accgtgcagg agctcgccaa ggcgatgagc gccctgggcc
    13321 cggacgacaa gcgcaagcac gtctcgtact tcaacgctcg catggccgag ctgaaaggct
    13381 ccaagtgatc gagtaccgcg acccccaggg ctcgcccgag tggctggccg cgcgccgcgg
    13441 cgtgatcaca gcgtcccggg cgaaggatgc ccgcgaccgt tcttccggtc tcgacgagcg
    13501 tcagacggcc tatgtccgcg cgatcctcgc cggccagtcg gagtccgagg cgctggccgc
    13561 cagcggctac aagaaggcgc ctacggccga ggcggtccgg gcgtgcctgg atgcccgtcg
    13621 catcgagaag aagtggagcg gcgcggcgat cagctacgcg caggatctcg cgcgcgagcg
    13681 ctgcggcggc gtgctgccgg aacagcgcga gggcttcgcg cagcgccagg gccatgagca
    13741 agagccgttc ggagtcatcg cctggatcgc gagcacgggc ctcgaggtcg aggaggtcgg
    13801 cttcgtcgcc accgatgacc gccgcttcgg tacgagcctc gaccgccgcg tcgtcggcaa
    13861 gaacgcggcg ctcgaggtca agacgatggt cagcagcgtc acgctgttcc gcgcgatcgt
    13921 ggacggcgac gtcagcgagt accgcgacca gtgcctgttc cagctctgga tgctgtcgct
    13981 cgactggatc gacctctgcc tctggtgccc cgacctgaac aagctgcaca tcgtccgcat
    14041 cgagcgcgac gaggacgagc tgcaggcgct cgaggatgac ctggtcgcct tcgacgcgct
    14101 cgtcaccttc tacgagctgg ccttgcgcag aaagctgggc atcgacgacg acctcctcaa
    14161 cggcgagccc gtctcggtgc ccgacgagcg tcccgcctcc gcctccccgc ccatcaagag
    14221 cgccgccgcg cccgcggccg gcccccgggc ctccgctcct gcgccggcca tccctgaact
    14281 ggagttctga cccatgaccg acgaagtcct ggagcgccac gccgcgccta ccaccctgcc
    14341 agcgcctgcg cctgaagtcg ccgtcgccga gaagccgctc acgatcgagc agaccatccg
    14401 cgtgcagttc gccgcgctcg agccgacgat gaccgccctg gccgcccgcc actcgaacat
    14461 cgcctgggac gtcacgacgc ccaagggcct gtcggacgcc aaggcggcgc gccacgaatt
    14521 gcgcgagagc ggccgcttct tcctgaaccg cagcctcgac gccaagaagc gagaggtcaa
    14581 cgacgtgaag acgatcctcg aatccgagac ggcgcgactg gtcgcgatcg tgaagcccgt
    14641 cgaggacgag atcaacgcga tcatcgtggc gcgcgagaac gagctcgctg ccgaaaaggc
    14701 cgagcgggaa cgcatcgctg cggagcgccg agcgcgcttc gaggcggaga tcgccaagat
    14761 caccggcatg gcggcgcgct gcgcggggat ctcggccgag cgaatcgcga acggcatccg
    14821 gctcgtcgag gacatgacct tcggcgagga gtgggccgag ttcgccgccg cggcggccaa
    14881 ggcccaggcc gagacgctcg ccgccatgcg caggctccac gccgaggcgc tcgaggccga
    14941 ggcgcgtgcg gctcgcgagg cggcgctcga agtcgagcgg caggagctcg cgcgccaagc
    15001 tgaagcgcag cgccaggagc
//
LOCUS       DASVLN010000317         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_16152, whole genome shotgun sequence.
ACCESSION   DASVLN010000317 DASVLN010000000
VERSION     DASVLN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16152"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..613
                     /locus_tag="VF453_07720"
     CDS             203..613
                     /locus_tag="VF453_07720"
                     /inference="COORDINATES: protein motif:HMM:NF016807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HEX7687578.1"
                     /translation="MLLQLFYVNKTAGALADVELQVLLGQAQIRNRRLDVTGILVKGD
                     GHFCQVLEGRPKAVEEVMARVRRDPRHRDVRVLSEQVITRREFGQWAMGLTVRSDLAD
                     EMDRLHRLGGLGGMPMREVIRRLTEPLPQAGSSG"
     gene            762..1256
                     /locus_tag="VF453_07725"
     CDS             762..1256
                     /locus_tag="VF453_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687579.1"
                     /translation="MDMTLIQGTITGLKAAADLAKGIMDLKTMAEVQGRVIELQAAIL
                     HAQSSALSANADQAQMAEEIRQLKAQLEKARGWETERQRYALQPIRGGGVLYALKSDQ
                     ARSGEPAHYLCTNCYEQGRKSIVSLAADKDGWTAWTCPVCKSRVLSGYRGASHARFAG
                     QPSD"
     gene            1392..1853
                     /locus_tag="VF453_07730"
     CDS             1392..1853
                     /locus_tag="VF453_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687580.1"
                     /translation="MLQFRKALESIAFGSLVANREAYASAHADFAINFKLKNVLKRVH
                     AQNPRAYPQPVIMTVTDVGKHIEGVKDGFLTRDEMEFLHDRTGDVLHTWNPYKPGARE
                     VDIQRPFSEWLARLQRLLDLHVIALLDGGLWLVQMNVMPEGRVHAFSCDPA"
     gene            complement(2089..2274)
                     /locus_tag="VF453_07735"
     CDS             complement(2089..2274)
                     /locus_tag="VF453_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687581.1"
                     /translation="MKIDLLFPVAIMSPATFIASGILFQCGAPGVAAITISIATCTAL
                     AWRLLSSKASNPASQRP"
     gene            complement(2271..2588)
                     /locus_tag="VF453_07740"
     CDS             complement(2271..2588)
                     /locus_tag="VF453_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX7687582.1"
                     /translation="MRMHNPPHPGETLREEVLPALGISVTEAAAQLGVTRAAFSRVIN
                     GRAGISPEMALRLERWLGVDHGGSASVWLGMQAAYDLWHAQKAARPALTKIKPIKHEE
                     LAA"
     gene            complement(2590..2868)
                     /locus_tag="VF453_07745"
     CDS             complement(2590..2868)
                     /locus_tag="VF453_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7687583.1"
                     /translation="MIKNFAHKGLEAFFTTGSKAGIQPSHAARLRRQLAQLDQASAPR
                     DMNIPGWKLHPLAGPLAGHWSVWVNGNWRLTFTFDGTDASIVDYQDYH"
     gene            complement(2894..3061)
                     /locus_tag="VF453_07750"
     CDS             complement(2894..3061)
                     /locus_tag="VF453_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687584.1"
                     /translation="MPTVIDRMGERERTPVHGLCRATFSTWANETAAARPDIRERRPD
                     PARGMTPNVDH"
BASE COUNT          515 a         1006 c         1137 g          548 t
ORIGIN      
        1 tgggcgcctg gggccggccg cgcgtcgtcg aacggctgct cggcggcacg acgcgcgcgg
       61 tgctcgaggg cgcggccgtg cccgtgctgt tctcgcactg acgacctccg ccggcgggcg
      121 acccgccgtc gcgcaggctg aagcccgagg cgcgccattt gccgaatccg gctcggatcc
      181 aacgatggcg tcaggaagcg cgatgctgct gcagttgttc tacgtgaaca agaccgccgg
      241 cgccttggcc gacgtcgagc tgcaggtgct gctcggccag gcgcagatcc gcaaccgccg
      301 gctcgacgtc accggcatcc tcgtgaaggg cgacggccat ttctgccagg tgctggaagg
      361 ccggccgaag gccgtcgagg aggtcatggc gcgcgtgcgc cgtgatccgc gccaccgcga
      421 cgtccgcgtc ctgtcggagc aggtcatcac gcgccgggag ttcggccagt gggcgatggg
      481 gctgaccgtc agaagcgacc tggccgacga gatggaccgg ctgcatcgcc tcggcggcct
      541 gggcggcatg cccatgcgcg aggtcatcag gcggttgacg gagccgctgc cgcaggcggg
      601 cagctcgggc tgatggccag ggagatcctg gcggaggggg cgtctgtcga gtcccgcatg
      661 gatcctgggg attcggatcc tgcttcgcct ttccgccggg ctgtccgcca cgagcgctct
      721 cgcgctatgc tggcggtcag ctcaactgtg ggaggacgcc gatggacatg accttgatcc
      781 aagggaccat caccggtctg aaagcggccg ctgatctcgc caagggcatc atggacctca
      841 agacgatggc cgaggtgcag ggcagagtca ttgagcttca agcggcgatc ctgcacgctc
      901 agtcgagtgc gctctcggcc aatgccgatc aagcgcaaat ggccgaggag atccggcagc
      961 tcaaggcgca gcttgagaag gcgagggggt gggagacaga gcgccagcgc tatgcgctgc
     1021 agccgattcg cggcggcggc gtcctgtatg cattgaagtc ggaccaggcg cgctcagggg
     1081 aacctgcgca ctacctctgc accaattgct acgagcaggg tcgaaagtcg attgtgagtc
     1141 tcgcggcgga caaggatggg tggactgcct ggacgtgccc ggtttgcaaa tcacgcgttc
     1201 tctctggcta ccgcggcgcc agccacgcca ggtttgccgg acagccaagc gactgacatg
     1261 ctccctgaaa ctacgccgca aggcacctac tgtcggctaa tggaggacgt gagggccgca
     1321 ttgcgacgat aggccgtgtc ctcgggggcg aacttccgct cggtgacccc ggcctgggaa
     1381 acgaggtcgt gatgctgcag ttccgcaagg cgttggagtc gatcgccttc ggctcgctcg
     1441 ttgcaaatcg tgaggcgtat gccagcgcac acgctgactt cgcgatcaat ttcaagctca
     1501 aaaacgtgct gaagcgagtc cacgcccaga atccgcgtgc ctaccctcaa ccagtcatca
     1561 tgaccgtgac cgatgtcggg aagcacattg agggggtcaa ggatggcttt ctcacgcggg
     1621 acgaaatgga gttcctgcac gaccgcacag gcgacgtcct gcacacctgg aacccttaca
     1681 agccgggtgc acgagaagtc gacatccagc gtccgttttc cgaatggctc gcgcgccttc
     1741 agcggctact tgatcttcac gtcatcgcat tactcgatgg cggtctctgg ttggttcaga
     1801 tgaatgtgat gcccgaaggc cgagtacatg ctttcagctg cgacccggcc taaacaaatg
     1861 cgttcggcgg cgtgaggcta ctccgtgaag tgcttcggcc tgcccaaagc ccacaccggg
     1921 ttcataccgt cgagggtcgg cgtctcgccg ttgggcgtgc gagatagcgc aacgagggtt
     1981 tcgctgcctg cggcgggaga attcgcctca gccatcgtgc acatcctttc agtgtgggtg
     2041 cgttgtggtc aggccgcgcg cggcacgctc gattccggtc gctttgcctc agggccgctg
     2101 agacgccgga ttcgatgcct tcgaggagag caggcgccac gcaagcgcgg tgcaggtcgc
     2161 gatcgagatg gtgatcgccg cgacgcccgg tgcgccgcac tggaacagga tgccgctcgc
     2221 gatgaacgtc gcggggctca tgatggcgac ggggaacagc aggtcgatct tcacgcagcg
     2281 agctcctcgt gcttgatcgg cttgatcttc gtgagcgccg gccgtgccgc cttctgcgcg
     2341 tgccagaggt cataggcggc ctgcatcccg agccagacgc tcgcgctgcc gccgtggtcg
     2401 acgccaagcc agcgctccag gcgcagggcc atctccggcg agatgccggc gcggccgttg
     2461 atgacgcggg agaacgccgc gcgcgtgacg cccagctgcg cggcagcctc ggtcacgctg
     2521 atgccgagtg cgggcagcac ctcctcgcgg agggtttcgc ccgggtgggg cgggttgtgc
     2581 atgcgcatgt cagtggtagt cctggtagtc gacgatggaa gcgtccgtcc cgtcgaaggt
     2641 gaaggtcagc cgccagttgc cgttgaccca gaccgaccag tggccggcca atggtcccgc
     2701 gagcgggtgc agcttccagc cggggatgtt catgtcccgg ggcgcactgg cctggtcgag
     2761 ctgcgcgagc tggcgccgaa gtcgggcggc gtggctcggc tggatgcccg ccttgctgcc
     2821 cgttgtgaag aaggcctcca ggcccttgtg agcgaagttc ttgatcacga cgaggactgt
     2881 atagtgtgac tgctcagtgg tcaacgttcg gcgtcatgcc gcgcgctgga tcgggacgac
     2941 gttcgcggat gtcgggccgc gccgcggcgg tctcgttggc ccaggtggag aaggtcgcgc
     3001 ggcacaggcc gtgcaccggg gtgcgctcgc gctcgcccat gcgatcgatg acggtgggca
     3061 tcgccatgtt cgagaccggc cgcgccggct ggccggcgca tcggcgaggg gaagacgaag
     3121 gcgtccgcgt cggcgtcggc cagggcgcgc acgccggtga gtgccgctag cgcgccgccg
     3181 gcctgcgcgg tgctgccgcg gtgccc
//
LOCUS       DASVLN010000318         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_230682, whole genome shotgun sequence.
ACCESSION   DASVLN010000318 DASVLN010000000
VERSION     DASVLN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230682"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..412
                     /locus_tag="VF453_07755"
     CDS             98..412
                     /locus_tag="VF453_07755"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX7687585.1"
                     /translation="MSTRLDAAKLPELLAQLPGWSLDPQAQSISRHLEFADFNAAFAF
                     MTRVALAAERRDHHPDWSNAYNVVDITLTTHDVGGLTMKDIELARFIDRTAAEASASR
                     HA"
     gene            complement(464..1084)
                     /locus_tag="VF453_07760"
     CDS             complement(464..1084)
                     /locus_tag="VF453_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687586.1"
                     /translation="MISKALLFRFESQTDEDTMDTFLEDLSAEVSWEKTTRAWFGIRY
                     MRGEFGVVAYFPDEAGRAAHLDGKVAQKIFAAEHRLMTAPPKVLPLEIFATKMPAVIE
                     GATKGLVLRFKAKKGRENDVIQLLRDAKPHIDAEAGTLAWVAFQYDPEHFGLFSVFAD
                     NGARFSHLVGWLPRELAKNALSLLGGMPEPHMVDIMAGMSRQDVAE"
     gene            complement(1234..2091)
                     /locus_tag="VF453_07765"
     CDS             complement(1234..2091)
                     /locus_tag="VF453_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7687587.1"
                     /translation="MTLRPPRLLAPWGLVLLVPMLLSTGGCARITTGSVGIVRHWDGQ
                     IDPVPVTGFKTTVLDSVIEVDTTETRVPLNGLHPADANGVLLDGLDMVVSFRLDPARV
                     PAFYVQTKELDSYTDESGRHVTTIGLHVLGNILQHSVQEVTKKEALTQLAANLGDYEH
                     AILAQAQSELDAGYPGVFHLIRVNVNHFTPPAAIRAQVEAIAALRVESQRIAQAQTLI
                     QQQSQLESAKAVLEANALRAAMEASHLSAEQLIAWKNARAYEAQARAIGETATRTVDA
                     ARQPPAAGR"
BASE COUNT          300 a          754 c          791 g          348 t
ORIGIN      
        1 cgcctgtcgc agcggagaac cctcgtcccc ggcagcggca tcgcgatggg cctagagtgg
       61 tcgcctcccc atcgttgcca cgagaagaag gcccgccatg tccacccgcc tcgacgccgc
      121 gaagcttccc gaactgctcg cccagctgcc gggctggtcg ctcgacccgc aggcgcagtc
      181 gatctcgcgc cacctcgagt tcgccgactt caacgccgcg ttcgcgttca tgacgcgcgt
      241 cgcgctggcg gccgagcggc gcgaccatca ccccgactgg tcgaacgcct acaacgtggt
      301 cgacatcacg ctcacgacgc acgacgtcgg cgggctgacc atgaaggaca tcgaactcgc
      361 acgcttcatc gaccgcacgg cggccgaggc gtcggcctcg cgccacgcct gatcgcgcgc
      421 ctcggcgggc gagggcggcc ccggcgcgcg ccggggcgcg ggctcattcg gcgacgtcct
      481 ggcgcgacat gccggccatg atgtcgacca tgtgcggctc gggcatgccg cccagcagcg
      541 agagggcatt cttcgcgagc tcgcgcggca gccagccgac caggtgcgag aagcgcgcgc
      601 cgttgtcggc gaagaccgag aacaggccga agtgctcggg gtcgtactgg aacgcgaccc
      661 aggcgagcgt gccggcctcg gcgtcgatgt gcggcttggc gtcgcgcagc agctggatga
      721 cgtcgttctc gcggcccttc ttggccttga agcgcagcac caggcccttg gtggcgccct
      781 cgatcacggc cggcatcttg gtggcgaaga tctccagcgg cagcaccttg ggcggcgccg
      841 tcatcaggcg gtgctccgcg gcgaagatct tctgcgcgac cttgccgtcc aggtgggccg
      901 cgcggccggc ctcgtcgggg aagtaggcga cgacgccgaa ctcgccgcgc atgtagcgga
      961 tgccgaacca ggcccgcgtg gtcttctccc aggacacctc ggcgctgagg tcctccagga
     1021 aggtgtccat cgtgtcctcg tcggtctgcg actcgaagcg gaagagcagg gccttgctga
     1081 tcattcggaa tcctttcgtt gaggtgcacg gcgggcgtgc ctggaacata tcctatacac
     1141 gatcgcgcgc gacgcgcccg ggtgttgtcc ctgcccgacg gcaggggtcg tgccgggtgc
     1201 gcatccgcgt cgtccgcggc gtgcgcccga ggctcacctg ccggccgcgg gcggctggcg
     1261 cgcggcgtcg acggtgcgcg tcgcggtctc tccgatcgcg cgcgcctgcg cctcgtaggc
     1321 gcgtgcgttc ttccaggcga tcagttgttc ggcgctcagg tgcgaggctt ccatcgcggc
     1381 gcgcagggca ttggcctcga gcaccgcctt cgcggactcg agctggctct gctgctggat
     1441 cagcgtctgc gcctgcgcga tgcgctgcga ctccacgcgc agggcggcga tcgcctccac
     1501 ttgcgcgcgg atcgccgccg gcggcgtgaa gtggttgacg ttgacgcgaa tcaggtggaa
     1561 caccccgggg tatccggcgt cgagttcgga ctgggcctgc gcgaggatcg catgctcgta
     1621 gtcgccgagg ttggccgcca gctgcgtgag cgcctccttc ttcgtcactt cctgcacgct
     1681 gtgctgcagg atgttgccca gcacgtgcag gccgatcgtc gtgacatggc ggcccgattc
     1741 gtcggtgtag gagtcgagct ccttggtctg gacgtagaag gccggcacgc gcgccggatc
     1801 gagccggaac gagaccacca tgtcgagccc gtccagcagc acgccgttgg cgtcggcggg
     1861 atgcaggccg ttgagcggca cgcgcgtctc ggtggtgtcg acctcgatca cggagtcgag
     1921 cacggtcgtc ttgaagccgg tgacgggcac ggggtcgatc tggccgtccc agtgccggac
     1981 gatgcccacc gagccggtgg tgatgcgtgc gcagccgccc gtcgagagca gcatcggcac
     2041 gagcaggacg aggccccagg gggcgagcag gcgcggcggg cgcagggtca tgatggtctt
     2101 cgaagcgccg gacgtctccc ggcggagcgt ggcgcccgcg cgcatcgcgc gcgaagcggt
     2161 cgccccgtcg ggcggcgcgt gcagtatcgc gtc
//
LOCUS       DASVLN010000319         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_446963, whole genome shotgun sequence.
ACCESSION   DASVLN010000319 DASVLN010000000
VERSION     DASVLN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446963"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..581)
                     /locus_tag="VF453_07770"
     CDS             complement(105..581)
                     /locus_tag="VF453_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7687588.1"
                     /translation="MPHKPPPPGWPQITAGLFYRDPARAIDWLVDAFGFEVRLKVEGE
                     PGEIVHSELVYGQGMIMVNDAHAAGGALEPGREWKAEHASPLDLGGRMNQSLCMFVDD
                     ADAHCANARAHGATIIAEPAVHDYGDDYWSDRTYGARDPEGHTWWFMQRLRSEPAK"
     gene            710..>2502
                     /locus_tag="VF453_07775"
     CDS             710..>2502
                     /locus_tag="VF453_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX7687589.1"
                     /translation="MTKILPGALLAAFLILQPAAPARAAQRVVLPDDVRPVAYRIDFT
                     PDAAHASFAATVEIDVDVRRPTARIVVDAADLAIDDASLAGEARAPAVSLDEKAQTAT
                     FAFSHALAPGRRTLVVRYHGRINDQGYGLFRLAWTTPQGPASALYTQFENTDARRFVP
                     CWDEPGIKATFALSAVVPQGLMAVGNMPVASTQALPGGLQRVRFATTPRMSSYLLFFA
                     LGDFERVHRVVDGVDVGVVVRRGAAASAGFALDAAAALLPWYDRWFGVRYPLPKMDMI
                     GGAGESPAFGAMENWGALFHFERDLLVDPARASQADRRRVFATIAHEMAHQWFGDFVT
                     MAWWDDLWLNEGFATWMETKAADALHPEWHASLDALADKQDALGPDARPGSHPVVAHI
                     VDADEAEGVFDAITYEKGAQAVRALEAAVGADAFRDGVRRYMRRHAYGNTTSDDLWAA
                     LDEGAAHPIAPVARDLVLQAGVPLVSELGARCDRGDTVVTLSQGRFAVDPADAAPRRW
                     RVPMAVSTLGHAAVRAIVAGPAPQQLRVPGCGPVVLDPGQAGYLRARYQPGDLAALTA
                     RFDELAPIDQLGLLADTAALARHGDVAVADW"
BASE COUNT          313 a          919 c          920 g          350 t
ORIGIN      
        1 cgggccgtcg cgcagcaggc cgacgacggc gcggcgcgtg ggctcggcca gggcctgcag
       61 cgtgcggtcg atcgcgccgt cgtcggacgc gggcgccgga cgggtcactt cgcgggttcg
      121 ctgcgaagcc gctgcatgaa ccaccacgtg tggccctcgg ggtcgcgtgc gccgtaggtg
      181 cggtcgctcc agtagtcgtc gccgtagtcg tggacggcgg gctccgcgat gatggtcgcg
      241 ccgtgcgcac gcgcgttggc gcagtgggca tcggcgtcgt cgacgaacat gcacagcgac
      301 tggttcatgc ggccgccgag gtccagcgga ctggcatgct cggccttcca ctcgcgcccg
      361 ggctccagcg cgccgccggc cgcgtgcgca tcgttgacca tgatcatgcc ctggccgtag
      421 acgagctcgc tgtgcacgat ctccccgggc tcgccctcga ccttgaggcg cacctcgaag
      481 ccgaaggcgt ccacgagcca gtcgatggcg cgcgcggggt cgcggtagaa aaggccggcg
      541 gtgatctgcg gccagccggg cggcggaggc ttgtggggca tggcgggctc cgtgagtcat
      601 caacgatggc gttgatcgta aacccaatcg ttaacggctt gcaagcgtgg cgaacctggc
      661 gcgacgcgac cgtctagact gcggcgctgt cgaacccgcc ccccgaagaa tgacgaagat
      721 cctgcccggc gcgctgctgg ccgcgttcct gatcctgcag cccgcggcgc ccgcgcgcgc
      781 cgcgcagcgc gtggtcctgc ccgacgacgt gcgcccggtc gcctaccgca tcgacttcac
      841 gcccgacgcg gcgcacgcga gcttcgcggc gaccgtcgag atcgacgtcg acgtgcggcg
      901 gccgaccgcg cgcatcgtcg tcgacgccgc ggacctcgcg atcgacgatg cctcgctcgc
      961 gggcgaggcg cgggcgccgg cggtctcgct cgacgagaag gcgcagaccg cgaccttcgc
     1021 cttttcgcac gcgctcgcac ccggccgccg cacgctcgtc gtgcgctacc acggccgcat
     1081 caacgaccag ggctacggcc tgttccgcct cgcgtggacc acgccccagg gccccgcgag
     1141 cgcgctctac acgcagttcg agaacaccga cgcgcgccgg ttcgtgccct gctgggacga
     1201 gcccggcatc aaggcgacgt tcgcgctgag cgccgtcgtg ccgcaagggc tgatggcggt
     1261 gggcaacatg ccggtggcct ccacgcaggc cctgccgggc ggcctgcagc gcgtgcgttt
     1321 cgcgaccacg ccgcgcatgt cgagctacct gctgttcttc gcgctcggcg acttcgagcg
     1381 cgtgcaccgc gtggtcgacg gcgtggacgt gggcgtggtc gtgcgccgcg gcgccgcggc
     1441 gagcgccggc ttcgcgctcg acgccgccgc cgcgctgctg ccctggtacg accgctggtt
     1501 cggcgtgcgc tacccgctgc ccaagatgga catgatcggc ggcgccggcg agagtcccgc
     1561 cttcggcgcg atggagaact ggggcgcgct gttccacttc gagcgcgacc tgctcgtcga
     1621 tcccgcgcgt gcgtcgcagg cggatcggcg gcgcgtgttc gcgaccatcg cgcacgagat
     1681 ggcgcaccag tggttcggcg acttcgtcac gatggcctgg tgggacgacc tgtggctcaa
     1741 cgagggcttc gcgacctgga tggagaccaa ggccgccgac gcgctgcatc ccgaatggca
     1801 cgcctcgctc gacgcgctcg ccgacaagca ggacgcgctc ggcccggacg cgcggccggg
     1861 gtcgcatccc gtggtcgcgc acatcgtcga cgcggacgag gccgagggcg tgttcgacgc
     1921 gatcacctac gagaaggggg cgcaggccgt gcgcgcgctg gaggccgcgg tcggcgccga
     1981 cgcctttcgc gacggcgtgc gccgctacat gcgccgccac gcctacggca acaccaccag
     2041 cgacgacctc tgggccgcgc tcgacgaggg cgcggcgcat cccatcgcgc cggtcgcgcg
     2101 cgacctggtg ctccaggccg gcgtgccgct ggtgtcggag ctcggcgcgc gctgcgatcg
     2161 cggcgacacc gtggtcacgc tgtcgcaggg ccgcttcgcg gtcgacccgg ccgatgcggc
     2221 cccgcgacgc tggcgggtac ccatggccgt atcgacgctg ggccatgcgg cggtgcgagc
     2281 gatcgtcgcc ggccccgcgc cgcagcaact gcgcgtgccg ggctgcgggc cggtggtgct
     2341 cgacccgggc caggctggct acctgcgcgc gcgctaccag ccgggcgatc tcgcggcact
     2401 gacggcgcgc ttcgacgaac tcgcgccgat cgaccagctc ggcctgctcg ccgacaccgc
     2461 ggcgctcgcg cgccacggcg acgtggcggt cgccgactgg at
//
LOCUS       DASVLN010000320         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_285514, whole genome shotgun sequence.
ACCESSION   DASVLN010000320 DASVLN010000000
VERSION     DASVLN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285514"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VF453_07780"
     CDS             <1..278
                     /locus_tag="VF453_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX7687590.1"
                     /translation="AEYAPGTPLGWHRDVPEFEDVFGVSLGSPALLRFRPWPPDAPKR
                     SDIVRLTAAPRSIYALRGPARWAWQHSVAPVEALRWSITFRTRRVGR"
     gene            complement(289..>1601)
                     /locus_tag="VF453_07785"
     CDS             complement(289..>1601)
                     /locus_tag="VF453_07785"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7687591.1"
                     /translation="LAASTAHPAPPPSPQATLDAIAADVDPQALHATIARLVDFGTRH
                     TLSETNSPTRGIGAARRWTKARFEEISRACGGCLEIVTPAQTVTGPRIPQPAVIEDVV
                     AIQRGTADPGRVVAITGHIDSRVTDVMDAVHDAPGADDDGSGVAAVLEAARVLSKRRF
                     AGTIVYGVLSGEEQGLYGGKLLADYAQAQGWRVEADLNNDIVGSSRGEDGVADNGVVR
                     VFSEGTKSTETPAQATHRRYNGGEVDSPSRNLARFVAGLAERTLTNFRAQLVYRTDRY
                     GRGGDQVPFLEHGFPALRFTEPHEDYRREHQDLRTEAGTAYGDTLAGVDFAYLAQVTR
                     LNAIALAALANAPAPVAGLAVEGAVTTDTRLHWTPSPGAACYRAWWRDTTSPTWQHVR
                     DVPAGATAVTLKDVDIDDFFFGVSAVSADGWESPVVFPGDAGSF"
BASE COUNT          193 a          581 c          612 g          215 t
ORIGIN      
        1 tggcggagta cgcgcccggc acgccgctcg gctggcaccg cgacgtgccc gagttcgagg
       61 acgtcttcgg cgtctcgctc ggcagcccgg cgctcctgcg ctttcggccc tggccgccgg
      121 acgccccgaa gcgctccgac atcgtccgcc tcacggccgc gccgcgctcg atctacgccc
      181 tgcgcgggcc ggcgcgctgg gcctggcagc acagcgtcgc gccggtcgag gcgctgcgct
      241 ggtcgatcac ctttcgcacg aggcgcgtgg gtcgctgagg ccgcgcactc agaagctgcc
      301 ggcgtcgccc gggaacacga ccggcgactc ccagccgtcg gccgagaccg ccgacacgcc
      361 gaagaagaag tcgtcgatgt cgacgtcctt cagcgtcacg gcagtcgcgc cggcggggac
      421 gtcgcgcacg tgctgccagg tcggcgaggt ggtgtcgcgc caccacgcgc gatagcaggc
      481 ggcgccgggc gagggcgtcc agtgcaggcg ggtgtcggtc gtcaccgcgc cctcgaccgc
      541 gagccccgcg accggcgccg gagcgttcgc gagcgcggcc agcgcgatcg cgttcagccg
      601 cgtcacctgc gcgaggtagg cgaagtcgac gccggccagg gtgtcgccat aggccgtgcc
      661 ggcctcggtg cgcaggtcct ggtgctcgcg gcggtagtcc tcgtgcggct cggtgaagcg
      721 cagcgccggg aagccgtgct cgaggaaggg cacctggtcg ccgccgcggc cgtagcggtc
      781 ggtgcgatag accagctgcg cgcggaagtt ggtcagcgtg cgctcggcca ggccggcgac
      841 gaagcgcgcg aggttgcgcg agggcgagtc cacctcgccg ccgttgtagc gccggtgcgt
      901 ggcctgcgcg ggcgtctcgg tcgacttcgt gccctccgag aagacgcgca cgacgccgtt
      961 gtccgcgacg ccgtcctcgc cgcggctgga gccgacgatg tcattgttga gatcggcctc
     1021 gacgcgccag ccctgcgcct gcgcgtagtc ggccagcagc ttgccgccgt acaggccctg
     1081 ctcctcgccc gagagcacgc cgtagacgat ggtgcccgcg aagcgccgct tcgacagcac
     1141 gcgcgcggcc tcgagcaccg cggccacgcc ggagccgtcg tcgtcggcgc ccggcgcatc
     1201 gtgcacggcg tccatcacgt cggtgacgcg cgagtcgatg tggccggtga tcgcgacgac
     1261 gcggcccgga tccgcggtcc cgcgctggat cgcgaccacg tcctcgatca cggccggctg
     1321 cgggatgcgc ggtccggtga ccgtctgcgc aggcgtgacg atctccaggc agccgccgca
     1381 ggcgcgcgag atctcctcga agcgggcctt tgtccagcgc cgcgcggcgc cgatgccgcg
     1441 cgtcggggaa ttcgtctcgg acagcgtgtg gcgggtgccg aagtcgacca ggcgcgcgat
     1501 cgtcgcgtgc agggcctgcg gatcgacgtc cgcggcgatc gcgtccagcg tggcctgcgg
     1561 cgacggcggc ggcgcgggat gcgcggtgga cgcggcgagc g
//
LOCUS       DASVLN010000321        10844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_447023, whole genome shotgun sequence.
ACCESSION   DASVLN010000321 DASVLN010000000
VERSION     DASVLN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10844
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447023"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..1774)
                     /locus_tag="VF453_07790"
     CDS             complement(248..1774)
                     /locus_tag="VF453_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX7687592.1"
                     /translation="MPTVIKAADLIESVAAALQYISYYHPADYIAHLARAYEREESPA
                     AKDAIAQILTNSRMSAEGKRPICQDTGIVNVFLKVGMDVRFEGCDAGIEAVVDEGVRR
                     GYLNPDNLLRASVLDDPLFARKNTKDNTPAVVHVQLVPGDKLDVTVAAKGGGSENKSK
                     FVMMNPSDSLVDWVLKTVPTMGAGWCPPGMLGIGVGGTAEKAMLLAKESLMEDIDMYE
                     LLQRGPSNKLEELRIELYEKVNALGIGAQGLGGLTTVLDIKIKTFPTHAASKPVAMIP
                     NCAATRHAHFVLDGSGPAFLEAPSRDLWPDVHWAPDYNKSRRVDLDTLTPEIVASWKH
                     GETLLLNGKMLTGRDAAHKRIQDMLAKGEPLPVDFRNRVIYYVGPVDPVRDEVVGPAG
                     PTTATRMDKFTEMMLANTGLIAMIGKAERGPVAIEAIKKHQSAYLMAVGGAAYLVSKA
                     IKQARVVGFADLGMEAIYEFDVVDMPVTVAVDAGGTSAHITGPKEWQARIGKIPVATA
                     "
     gene            1968..2384
                     /locus_tag="VF453_07795"
     CDS             1968..2384
                     /locus_tag="VF453_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687593.1"
                     /translation="MTPVADTLWRWIETNLMKNRTKWSNTLDYGVTVDEDDMDSMVSE
                     QFGGYKKNYWFQIGDVDYKMRCLPLQNKNTREKHAYGSVKGGGENKVNILCYQDGVDG
                     VKFNLHIQVRRLAQALDARRRAANRDAEAEGGWQTA"
     gene            complement(2478..3110)
                     /locus_tag="VF453_07800"
     CDS             complement(2478..3110)
                     /locus_tag="VF453_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HEX7687594.1"
                     /translation="MTPDLPSRPTPVRLRPLPRLIFASRWLQLPLYLGLIVAQVLYAE
                     HFLVELTHLLQASLGDGQALQALVASIGYHPTAAAADSAVALPPVTSLTETVIMLAVL
                     GLIDVVMISNLLIMVIVGGYETFVSRLDLDGHRDQPEWLDHVNASVLKVKLGTAIIGI
                     SSIHLLKTFINAGAYDAKVLMWQTLIHLAFLLSALALAFVDRLLPQHGSH"
     gene            3435..5414
                     /gene="acs"
                     /locus_tag="VF453_07805"
     CDS             3435..5414
                     /gene="acs"
                     /locus_tag="VF453_07805"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX7687595.1"
                     /translation="MSAADQTSHETHVYDPPAELAAHAWVSGMDAYRELCDEAEADHE
                     GFWARRAREFLSWKTPFTKTLDASDAPFFKWFEDGTLNVSYNCLDRQVEAGLGDKTAI
                     IFEADDGAVTRVSYRELLARTCRMANALKARGVRKGDRVVIYMPMSVEGVVAMQACAR
                     IGATHSVVFGGFSAQSLRDRIEDAGAVMVITADEQQRGGKQLPLKAIVDEALALGGCD
                     TIRDVVVYRRTGGAVAWNAVDRWMHELVADQADTCEPEWVGAEHPLFLLYTSGSTGKP
                     KGVQHSSAGYLLHAALTTKWTFDLKDDDVFWCTADIGWVTGHTYITYGPLALGGTEVV
                     FEGVPTYPDAGRFWKMIQDHKVSKFYTAPTAIRSLIKAAEGNPKVHPRNYDLSSLKVL
                     GSVGEPINPAAWEWYHEHVGGGRCPIVDTFWQTETGGHMITPLPGATPLVPGSCTLPL
                     PGIAAAVVDETGNDVDNGTGGILVVKKPWPSMIRTIWGDPERFRKSYYPPELKGYYLA
                     GDGAIRDAKTGYFTITGRIDDVLNVSGHRMGTMEIESALVSHTALVAEAAVVGRPDDT
                     TGEAIVAFVVLKRPRPTGEEARAIAKELRDWVGKEIGPIAKPKDIRFGDNLPKTRSGK
                     IMRRLLRAVAKGEQITQDTSTLENPAILEQLAENN"
     gene            5541..5828
                     /locus_tag="VF453_07810"
     CDS             5541..5828
                     /locus_tag="VF453_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04438 family Trp-rich protein"
                     /protein_id="HEX7687596.1"
                     /translation="MWFVAIGVLMIVGNLTGYGPTAKWNWEFTGDVWKFALPFVAAAF
                     WWAWADASGLSKRRAMRKDEERKQDRRDRNIDAMGLGHLHKHRDGRKRAKR"
     gene            complement(5890..7572)
                     /gene="ilvD"
                     /locus_tag="VF453_07815"
     CDS             complement(5890..7572)
                     /gene="ilvD"
                     /locus_tag="VF453_07815"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7687597.1"
                     /translation="MAANRRSRNVTEGVARAPNRSMYYGMGYQAEDFAKPMIGVANGH
                     STITPCNSGLQPLADAAVDALKAEGANALLFGTPTISDGMAMGTEGMKYSLVSREVIA
                     DCVETCVGGQWMDGVLVIGGCDKNMPGGMMGMLRANVPAIYVYGGTILPGHYKGEDLN
                     IVSVFEAVGQYTAGRMSDEDFREIERRAIPGPGSCGGMYTANTMSSAFEALGMSLPYS
                     STMANVEGEKAESARESARVLVEAVQADLKPRDIVTREAIENAVAVIMATGGSTNAVL
                     HFLAIAHAAGVEWTIDDFERVRRRTPVLCDLKPSGRFLAIDLHRAGGIPQVMRLLLDA
                     GLLHGHCMTITGRTIAETLADVPARPRADQPVIRTIAEALYPDGHLAILRGNLAVDGC
                     VAKITGLRNPVITGPARVFDDEQSALAAIMAGRIVAGDVMVLRYLGPKGGPGMPEMLA
                     PTGALIGQGLGESVGLVTDGRFSGGTWGMVVGHVAPEAYEGGAIALVHEGDAITIDAH
                     RRLLQLHVDDAELARRRAAWTQPAPRYTRGVLAKFARNAASASFGAVLDKFD"
     gene            7719..8555
                     /gene="lgt"
                     /locus_tag="VF453_07820"
     CDS             7719..8555
                     /gene="lgt"
                     /locus_tag="VF453_07820"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7687598.1"
                     /translation="MLVHPQFDPVAVSFGPWEVAGHVLGPFQVHWYGLTYMVAFALFT
                     VLASRRAAYPWFAREGWVRRDVEDMLFYGVLGVVLGGRLGYVLFYKPGYYAQHLSEIP
                     AVWKGGMSFHGGMLGVILAMMLFAWARKRPWLQVTDLIAPCVPLGLASGRIGNFINGE
                     LWGRFADPSLPWAMVFPQSGSPLPRHPSELYEFLGEGLLLFALMWIYGRKERGRGQVS
                     GLFLMGYGVARFVVEFFREPDDFLGLLALHMSMGQWLCVPMIAAGAALFAWGARQPRG
                     KA"
     gene            8635..9354
                     /gene="dnaQ"
                     /locus_tag="VF453_07825"
     CDS             8635..9354
                     /gene="dnaQ"
                     /locus_tag="VF453_07825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX7687599.1"
                     /translation="MRQIFLDTETTGLSPENGDRVIEIGCIEMENRRVTGRHLHFYLN
                     PERKNSEDAVKVHGLTDEFLADKPLFAHVVDEFLEFCADAEIIIHNAGFDVGFLNEEL
                     RRVGRPKFTTHVRGVVDTLVMAREMFPGKSNSLDALCKRLEVDNTNRTLHGALIDADL
                     LAQVYVNLTRGQDSLVMELAEEQSGPSGDAGPIDLSVFVLPVLAATADELAGHERVLG
                     EIDKASGGKTIWRAQTGNAVA"
     gene            9393..9467
                     /locus_tag="VF453_07830"
     tRNA            9393..9467
                     /locus_tag="VF453_07830"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9425..9427,aa:Val,seq:cac)
     gene            complement(9514..>10844)
                     /locus_tag="VF453_07835"
     CDS             complement(9514..>10844)
                     /locus_tag="VF453_07835"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7687600.1"
                     /translation="VRPADAPGAPTMVYALLYIAVLLFLWYRHIRKRRRKESAHAAEL
                     REAVAKGMTEPPSLHPVIDPVRCIGSASCVRACPEEALGIIKGKAVLTNAAACIGHGA
                     CLAACPVDAITLVFGTEKRGMDIPNVRPNFETNVPGIFIAGELGGMGLIRKAAEQGRQ
                     AIEAVKARKSPGAADHDVVIVGGGPAGISAGLAAIAAGLRYKVIEQEDSLGGTVYHYP
                     RNKIAMTAPVKLAIVGTVRMGEIQKEALLEFWQDVVRKTGLQLSLRERMEHIERRPDG
                     SFAVHTSAGVHTARSVVLAIGRRGSPRKLDVPGEESSKVVYRLIDRQQYRGRAVVVVG
                     GGDSALEAAVALAEQPQTDVTLSYRSAAFSRVKAKNRERVEELEKSGRLRVLLESNVV
                     SIDEHAVTIERHGEATSMKNDAVIVCAGGILPTPLLQKVGIEFETKFGAR"
BASE COUNT         1595 a         3759 c         3874 g         1616 t
ORIGIN      
        1 gtgggcgacc cgcgcggggc cgaggcgacg agcggcagcc tcgcggatcc accggctggc
       61 gcgccgcgcg cgccggacag gccggcggcc acgttcatcg gcagcgacgg cgagccgcag
      121 tcctgaggat cgcgcgggca gcgttcggga atgcgggcgt cgcgttcgcg accctggccg
      181 ccgtgccggc gatgtcgagg gcgcattgcc cggcgtcgac gcggggagct cgacgccgat
      241 agcgggatca ggccgtcgcg accgggatct tgccgatccg ggcctgccac tccttcgggc
      301 cggtgatgtg ggccgaggtg ccgccggcat cgaccgcgac ggtcacgggc atgtcgacga
      361 cgtcgaactc gtagatcgcc tccatgccga ggtccgcgaa gcccacgacc ctggcctgct
      421 tgatcgcctt ggagacgagg taggccgcgc cgccgacggc catcaggtag gccgactggt
      481 gcttcttgat cgcctcgatg gcgaccgggc cgcgctcggc cttgccgatc atcgcgatga
      541 ggccggtgtt ggcgagcatc atctcggtga acttgtccat acgcgtggcg gtggtcgggc
      601 cggcggggcc gacgacctcg tcgcgcaccg gatcgaccgg gccgacgtag tagatgacgc
      661 ggttgcggaa gtcgaccggc agcggctcgc ccttggccag catgtcctgg atgcgcttgt
      721 gcgcggcgtc gcggcccgtc agcatcttgc cgttcagcag cagcgtctcg ccgtgcttcc
      781 aggacgcgac gatctcgggc gtcagcgtgt ccaggtcgac gcgccggctc ttgttgtagt
      841 ccggggccca gtggacgtcg ggccacaggt cgcggcttgg cgcctcgagg aaggccgggc
      901 ccgagccgtc cagcacgaag tgcgcgtggc gcgtggcggc gcagttgggg atcatcgcca
      961 ccggcttgga cgcggcatgc gtcgggaagg tcttgatctt gatgtccagc acggtcgtca
     1021 ggccgcccag gccctgcgcg ccgatgccca gcgcgttgac cttctcgtac agctcgatgc
     1081 gcagctcctc gagcttgttc gaggggccgc gctgcagcag ctcgtacatg tcgatgtctt
     1141 ccatcagcga ttccttggcg agcagcatcg ccttctcggc cgtgccgccg acgccgatgc
     1201 ccagcatgcc cggcgggcac cagccggcgc ccatcgtcgg caccgtcttg agcacccagt
     1261 cgaccaggct gtcgctcggg ttcatcatca cgaacttgct cttgttctcc gagccgccgc
     1321 ccttggccgc gacggtcacg tcgagcttgt cgcccggcac gagctgcacg tgcacgacgg
     1381 cgggcgtgtt gtccttcgtg ttcttgcgcg cgaacagggg gtcgtccagc acggaggcgc
     1441 gcagcaggtt gtccgggttc aggtagccgc ggcgcacgcc ctcgtcgacg accgcctcga
     1501 tgccggcgtc gcagccctcg aagcgcacgt ccatgccgac cttgaggaac acgttgacga
     1561 tgccggtgtc ctggcagatc gggcgcttgc cctcggcgct catgcgcgag ttggtcagga
     1621 tctgggcgat cgcgtccttg gccgcggggc tctcctcgcg ctcgtaggcg cgggcgaggt
     1681 gggcgatgta gtcggccggg tggtagtacg agatgtactg cagggcggcg gcgacggact
     1741 cgatcaggtc ggcggctttg atgacggtgg gcatgggcgt gcgggcggga aggctggcgg
     1801 gaatcccgca ttatccgccg cctgcgtgcc gaagcgccgc gctcgggctt ggggcccggg
     1861 cgagcaatcc ggcattgcgg atgtcacacg gattgtttca aattggcggg ttccgcggcg
     1921 cgtccgcggc gcccccggat tccccaaccc ctgaggagag agacgacatg acccccgttg
     1981 cggacacgct ctggcgctgg atcgagacca acctgatgaa gaacaggacg aagtggagca
     2041 acacgctcga ctacggcgtg acggtcgatg aggacgacat ggacagcatg gtcagcgagc
     2101 agttcggcgg ctacaagaag aactactggt tccagatcgg cgacgtcgac tacaagatgc
     2161 gctgcctgcc gctgcagaac aagaacacgc gcgagaagca cgcctacggc agcgtcaagg
     2221 gcggcggcga gaacaaggtc aacatcctgt gctaccagga cggcgtcgac ggcgtgaagt
     2281 tcaacctgca catccaggtg cgccggctgg cgcaggccct cgacgcgcgc cgccgcgcgg
     2341 ccaatcggga cgcggaggcg gaaggcggct ggcagaccgc ctgacctcag ccggcgtcgg
     2401 cgagcgggaa cgggcgctcg acgaacgcgc gccggaagcc ccctgcgcgc ctcgacgccc
     2461 tggggggccg ccggcgctca gtgcgacccg tgctgcggca gcagccggtc gacgaaggcc
     2521 agcgccagcg cggacagcag gaaggcgagg tggatcagcg tctgccacat cagcaccttg
     2581 gcgtcgtagg cgccggcgtt gatgaaggtc ttgagcaggt ggatcgagct gatgccgatg
     2641 atcgccgtac cgagcttgac cttcagcacc gaggcgttga cgtggtccag ccactcgggc
     2701 tggtcgcggt ggccgtccag gtcgaggcgg gagacgaagg tctcgtagcc gccgacgatc
     2761 accatgatga gcaggttcga gatcatcacg acgtcgatca accccagcac cgccagcatg
     2821 atcacggtct cggtgaggga ggtcacgggc ggcagcgcga cggcgctgtc ggccgcggcg
     2881 gcggtcgggt ggtagccgat gctcgcgacg agcgcctgca gggcctggcc gtcgcccagg
     2941 ctcgcctgca gcaggtgggt gagctcgacg aggaagtgct ccgcgtagag cacctgcgcg
     3001 acgatgagcc cgaggtacag cggcagttgc agccagcggc tcgcgaagat caggcggggc
     3061 agcgggcgca ggcggacggg cgtgggtcgg gagggcaggt cgggcgtcat ggggatcgtc
     3121 tcgcggcggg gcggcggttc ggcgtcgggg cattgtaggc agcggcgccg acgaaatgac
     3181 ggccgccggg cgccggcgcg cggcgtaccg aacactttcc ggcgcggccg ccccgcgcgc
     3241 tccaaacgcg gcgcatgcgc aggcgtgcgc ggccgccccg tgccccggct gccgctagga
     3301 tcgtcgcttc gccccagtca gccatcggta agagctggcg tataacgttc cccgcgacga
     3361 tcgcccgcac ccggcccccg cgccggagag gcgggcggcg acggatccgc acgagttccc
     3421 aggagacaac cgacatgagc gccgcagacc agacctccca cgaaacgcac gtctacgacc
     3481 cgcccgccga gctcgcggcc catgcctggg tcagcggcat ggacgcgtac cgcgagctct
     3541 gcgacgaggc cgaggccgac cacgaaggct tctgggcgcg ccgggcgcgc gagttcctga
     3601 gctggaagac gcccttcacc aagacgctgg atgcgagcga cgcgccgttc ttcaagtggt
     3661 tcgaggacgg caccctcaac gtctcctaca actgcctcga tcgccaggtc gaggccggcc
     3721 tcggcgacaa gacggcgatc atcttcgagg ccgacgacgg cgccgtcacg cgcgtgagct
     3781 accgggagtt gctcgcccgc acctgccgca tggccaacgc gctcaaggcg cgcggcgtgc
     3841 gcaagggcga ccgcgtcgtg atctacatgc cgatgtcggt ggagggcgtg gtcgcgatgc
     3901 aggcctgcgc gcgcatcggc gcgacgcact cggtggtctt cggcggcttc tcggcgcaga
     3961 gcctgcgcga ccgcatcgag gacgccggcg cggtgatggt gatcacggcc gacgagcagc
     4021 agcgcggcgg caagcaactg ccgctgaagg ccatcgtcga cgaggcgctg gcgctcggcg
     4081 gctgcgacac gatccgcgac gtggtcgtct accggcgcac gggcggggcg gtcgcctgga
     4141 acgcggtcga ccgctggatg cacgagctcg tggccgacca ggccgacacc tgcgagccgg
     4201 aatgggtcgg cgcggagcac ccgctgttcc tgctctacac ctcgggctcg acgggcaagc
     4261 ccaagggcgt gcagcactcc agcgccggct acctgctgca tgcggcgctg acgacgaagt
     4321 ggaccttcga cctgaaggac gacgatgtct tctggtgcac cgccgacatc ggctgggtca
     4381 ccggccacac ctacatcacc tacgggccgc tcgccctggg cggcaccgag gtcgtgttcg
     4441 agggcgtgcc cacctacccg gacgcgggcc ggttctggaa gatgatccag gaccacaagg
     4501 tgtccaagtt ctataccgcg ccgacggcga tccgctcgct gatcaaggcg gccgagggca
     4561 accccaaggt gcatccgcgc aactacgacc tgtccagcct caaggtgctg ggctcggtgg
     4621 gcgagccgat caaccccgcc gcctgggagt ggtaccacga gcacgtcggc ggcggccgct
     4681 gcccgatcgt cgacaccttc tggcagaccg agaccggcgg ccacatgatc accccgctgc
     4741 cgggcgcgac gccgctggtg ccgggctcct gcacgctgcc gctgcccggc atcgcggccg
     4801 cggtggtcga cgagacgggc aacgacgtcg acaacggcac cggcggcatc ctggtcgtga
     4861 agaagccctg gccgtcgatg atccgcacga tctggggcga cccggaacgc ttcaggaaga
     4921 gctactaccc gccggagctc aagggctact acctcgcggg cgacggcgcg atccgcgacg
     4981 cgaagaccgg ctacttcacg atcaccggcc gcatcgacga cgtgctcaac gtctccgggc
     5041 accgcatggg gacgatggag atcgagtcgg cgctggtctc gcacacggcg ctcgtcgccg
     5101 aggcggcggt ggtcggccga cccgacgaca cgaccggcga ggcgatcgtc gccttcgtcg
     5161 tcctgaagcg gccgcgcccg accggcgagg aggcgcgggc gatcgccaag gagctgcgcg
     5221 actgggtcgg caaggagatc ggcccgatcg ccaagcccaa ggacatccgc ttcggcgaca
     5281 acctgcccaa gacgcgctcg ggcaagatca tgcgccgcct gctgcgcgcc gtggccaagg
     5341 gcgagcagat cacgcaggac accagcacgc tcgagaaccc cgccatcctc gagcagctcg
     5401 ccgagaacaa ttgaacgccc gggccggcgg cggcggcgcg cgcccggctc cgccgcgcgc
     5461 gcttgctgct acattcgggc cgttcgcgca tcccccaacg tcagcgcggt cgcgcgcgcc
     5521 ggccgccgag gtccctgtcc atgtggttcg tcgccatcgg tgtgctcatg atcgtcggca
     5581 acctgaccgg ctacggcccc acggccaagt ggaactggga gttcaccggc gacgtctgga
     5641 agttcgcgct gccgttcgtc gccgcggcgt tctggtgggc atgggcggac gcgagcgggc
     5701 tgtccaagcg ccgcgcgatg cgcaaggacg aggaacgcaa gcaggatcgg cgcgaccgca
     5761 acatcgacgc gatgggcctc ggccacctgc acaagcaccg cgacggtcgc aagcgcgcga
     5821 agcgctgagc gcaggtcgcg caggacgggg cgccgcgggc gcccttcgcg tttccgggcg
     5881 cgcccggcct caatcgaact tgtcgagcac cgccccgaag ctcgcgctgg cggcgttgcg
     5941 cgcgaacttg gccaggacgc cgcgggtgta gcgcggcgcc ggctgcgtcc aggcggcgcg
     6001 gcggcgggcc agctcggcgt cgtcgacgtg cagctgcagc agccgccggt gggcgtcgat
     6061 ggtgatcgcg tcgccttcgt gcaccagcgc gatcgcgccg ccctcgtagg cctcgggggc
     6121 gacgtggccg acgaccatgc cccaggtgcc gcccgagaag cgcccgtcgg tgacgaggcc
     6181 gaccgactcg cccagcccct ggccgatcag cgcgcccgtg ggcgccagca tctcgggcat
     6241 gcccgggccg cccttgggac cgaggtagcg cagcaccatc acgtccccgg cgacgatgcg
     6301 cccggccatg atcgcggcga gggcggactg ctcgtcgtcg aacacgcgcg cggggcccgt
     6361 gatcacgggg ttcctcagtc cggtgatctt ggcgacgcag ccatccacgg cgaggttgcc
     6421 gcgcaggatc gcgaggtgcc cgtcgggata cagcgcctcg gcgatcgtgc ggatcaccgg
     6481 ctggtcggcg cgcggccgcg ccggcacgtc ggcgagcgtc tcggcgatgg tccggcccgt
     6541 gatggtcatg cagtggccgt gcagcaggcc ggcgtcgagc agcagccgca tgacctgcgg
     6601 gatgccgccg gcgcggtgca ggtcgatggc gaggaagcgt ccgctcggct tcaggtcgca
     6661 cagcaccggg gtgcggcggc gcacgcgctc gaagtcgtcg atcgtccact cgacgccggc
     6721 cgcgtgggcg atcgcgagga agtgcagcac ggcgttggtc gacccgcccg tggccatgat
     6781 cacggcgacg gcgttctcga tcgcctcgcg cgtcacgatg tcgcgcggct tcaggtcggc
     6841 ctggacggcc tcgaccagca cgcgcgccga ttcgcgggcg ctctcggcct tctcgccctc
     6901 gacgttggcc atcgtcgagg agtagggcag cgacatgccc agcgcctcga acgcggacga
     6961 catcgtgttg gccgtgtaca tgccgccgca ggagcccggg ccggggatcg cgcggcgctc
     7021 gatctcgcgg aagtcctcgt cgctcatgcg gccggccgtg tactggccca ccgcctcgaa
     7081 cacgctgacg atgttgaggt cctcgccctt gtagtgcccc ggcaggatgg tgccgccgta
     7141 gacgtagatc gccggcacgt tggcgcgcag catgcccatc atcccgccgg gcatgttctt
     7201 gtcgcagccg ccgatgacca gcacgccgtc catccactgg ccgccgacgc aggtctccac
     7261 gcagtcggcg atcacctcgc gcgagacgag gctgtacttc atgccctcgg tgcccatggc
     7321 catgccgtcg gagatcgtgg gcgtgccgaa caggagcgcg ttggccccct cggccttcag
     7381 cgcgtcgacg gccgcgtcag ccagcggctg cagcccggag ttgcagggcg tgatcgtcga
     7441 gtgcccgttg gccacgccga tcatgggctt ggcgaagtcc tcggcctggt accccatgcc
     7501 gtagtacatg gagcggttgg gcgcgcgggc gacgccctcg gtgacgttgc gcgatcggcg
     7561 atttgcggcc atggcggtct cctggtggcg gcgcacgcgg ccagctcggc ggcgggcgcg
     7621 ttcttggcga ggggccagta tccgtgcgcc ggcggcggcg tgtccaacgc ttcgtcgcgc
     7681 ggctcggcga caatcgcgcg ctcacaagga gttcgccgat gctcgtccac ccgcagttcg
     7741 atccggtcgc cgtgtccttc gggccctggg aggtcgccgg gcacgtcctc gggccgttcc
     7801 aggtgcactg gtacggactc acctacatgg tcgcgttcgc gctgttcacg gtgctcgcga
     7861 gccggcgtgc ggcctatccg tggttcgcgc gcgaaggctg ggtgcggcgc gacgtcgagg
     7921 acatgctgtt ctacggcgtg ctgggcgtcg tgctgggcgg gcggctcggc tacgtgctgt
     7981 tctacaagcc cggctactac gcgcagcacc tgtcggagat cccggcggtc tggaagggcg
     8041 gcatgtcctt ccacggcggc atgctgggcg tgatcctcgc gatgatgctg ttcgcgtggg
     8101 cgaggaagcg tccctggctg caggtcaccg acctcatcgc gccgtgcgtg ccgctgggcc
     8161 tggcgagcgg gcgcatcggc aacttcatca acggcgagct gtggggccgc ttcgccgacc
     8221 cgtcgctgcc ctgggcgatg gtgttcccgc agtcgggctc gccgctgccg cgccatccgt
     8281 cggagctcta cgagttcctc ggcgagggcc tgctgctgtt cgcgctgatg tggatctacg
     8341 gccgcaagga gcgcgggcgc gggcaggtct cgggcctgtt cctcatgggc tacggcgtcg
     8401 cccgcttcgt cgtcgagttc ttccgcgagc ccgacgactt cctcggcctg ctcgcgctgc
     8461 acatgagcat gggccagtgg ctgtgcgtgc cgatgatcgc cgccggcgcc gccctgttcg
     8521 cgtggggcgc gcgccagccg cgcggcaagg cgtgaggttt cgcgcggccc ggcgccgggc
     8581 cgtgcgccgc atccgagcga caatcgcggc tgccgccgcc cacgaccgcc tgccatgcgc
     8641 cagatcttcc tcgacaccga aaccaccggc ctgagccccg agaacggcga ccgcgtcatc
     8701 gagatcggct gcatcgagat ggagaaccgg cgcgtcaccg gccgccacct gcacttctat
     8761 ctcaaccccg agcgcaagaa cagcgaggac gcggtcaagg tgcacggcct gaccgacgag
     8821 ttcctcgccg acaagccgct gttcgcgcac gtcgtcgacg agttcctcga gttctgcgcc
     8881 gacgccgaga tcatcatcca caacgccggc ttcgacgtcg gcttcctcaa cgaggaactg
     8941 cgccgcgtcg gccgcccgaa gttcacgacc cacgtgcgcg gcgtcgtcga cacgctcgtg
     9001 atggcgcgcg agatgttccc cggcaagtcg aactcgctcg acgcgctgtg caagcgcctg
     9061 gaggtcgaca acaccaaccg cacgctgcac ggcgcgctga tcgacgcgga cctgctcgcg
     9121 caggtctacg tcaacctcac gcgcggccag gactcgctcg tgatggagct cgccgaggag
     9181 cagtcgggcc ccagcggcga cgccggcccg atcgacctga gcgtcttcgt cctgccggtc
     9241 ctcgccgcca ccgccgacga actcgccggc cacgagcgcg tgttgggcga gatcgacaag
     9301 gccagcggcg gcaagacgat ctggcgtgca caaacgggaa atgctgtggc ataattcaag
     9361 gctcgcctga acaccggggc gaacgaaata ccgggtggtt agctcagcgg tagagcactg
     9421 ccttcacacg gcaggggtca caggttcaat ccctgtacca cccaccagac gcgaagcccc
     9481 gcaggtctcc ggacttgcgg ggcttttcgc ttttcagcgc gcgccgaact tcgtctcgaa
     9541 ctcgatgccg accttctgca gcagcggcgt cggcaggatg ccgccggcgc agacgatgac
     9601 ggcgtcgttc ttcatcgacg tggcctcgcc gtgccgctcg atggtgacgg cgtgctcgtc
     9661 gatcgagacg acgttggact ccagcagcac gcgcaggcgg cccgacttct cgagttcctc
     9721 cacgcgctcg cggttcttcg ccttgacgcg gctgaaggcg gcgctgcggt aggacagcgt
     9781 cacgtcggtc tgcggctgct cggccagcgc gacggcggcc tccagcgcgc tgtcgccgcc
     9841 gccgaccacc acgaccgccc ggccgcggta ctgctgccgg tcgatgagcc ggtagacgac
     9901 cttggacgat tcctcgcccg gcacgtccag cttgcgcggc gagccgcggc ggccgatggc
     9961 cagcaccacc gaccgcgccg tgtggacccc ggcgctggtg tgcacggcga agctgccgtc
    10021 gggccggcgc tcgatgtgct ccatgcgctc gcgcagcgac agctgcagcc cggtcttgcg
    10081 caccacgtcc tgccagaact cgagcagcgc ctccttctgg atctcgccca tcctgacggt
    10141 gcccacgatc gcgagcttga ccggcgcggt catcgcgatc ttgttgcgcg ggtagtggta
    10201 gacggtgccg cccagggagt cctcctgctc gatcaccttg tagcgcaggc ccgctgcgat
    10261 ggccgcgagc ccggccgaga tcccggccgg tccgccgccg acgatgacga cgtcatggtc
    10321 ggccgcgccc ggggacttgc gcgccttgac ggcctcgatg gcctggcgcc cctgctcggc
    10381 ggccttgcgg atcaggccca tgccgcccag ctcgccggcg atgaagatgc ccgggacgtt
    10441 ggtctcgaag ttcgggcgca cgttggggat gtccatgccg cgcttctcgg ttccgaagac
    10501 gagcgtgatc gcgtcgaccg ggcaggccgc gaggcacgcg ccgtggccga tgcaggcggc
    10561 cgcgttcgtg agcaccgcct tgcccttgat gatgcccagc gcctcctcgg ggcaggcgcg
    10621 cacgcacgac gccgacccga tgcaacgcac cgggtcgatc accggatgca gcgaaggcgg
    10681 ctccgtcatg cccttggcga cggcctcccg cagttccgcc gcgtgcgcgg actccttgcg
    10741 gcggcgcttg cggatgtgcc ggtaccacag gaacagcagc accgcgatgt agagcagcgc
    10801 gtagaccatg gtgggcgctc ccggagcgtc ggcagggcgg acgg
//
LOCUS       DASVLN010000322         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_178190, whole genome shotgun sequence.
ACCESSION   DASVLN010000322 DASVLN010000000
VERSION     DASVLN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178190"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2237
                     /locus_tag="VF453_07840"
     CDS             <1..2237
                     /locus_tag="VF453_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7687601.1"
                     /translation="AHWIEWQHRAAGGDVGADAGAIHSVGRDITDRKLLERELLSSEQ
                     RYRSLFDHMQAGFALHEVVLDDAGRVVDVVYLAVNAPHARMMGFDPERMTGARLSELL
                     PAGAQAAASEWIAIFSRVALDGETISFERRAYDPPRWFSVVAYRPAPGQFATIVEDIS
                     ERKRAQEEMQAQHEQLRVTLHSIGDAVITTDNAGRVQYLNPVAERLTGWRDADARDRA
                     LEAVYHPVDEVSREPLPNPVAACLAGTPARADADDAGPGAEAPHAVLASRHGSEAGIE
                     QTVAPIRAADGSVLGAVLVFRDVTDRRRMAREMSYRATHDALTGLVNRSDFESRLHHA
                     LRLAHADGSVHALMYVDLDQFKLVNDACGHAAGDQLLRQVAALIKQCVRSRDTLARLG
                     GDEFGVILQFCSIEQGQRVAQQICERMDEFRFAHGERRFRVGASVGLVPLDSRWPTAA
                     SAMQAADAACYAAKEAGRNRVHAWFDADESLRLRSGEMQWVARLEQAVDEDRFELFAQ
                     RIQPIRAASSGLHCEVLLRLRGPDGRLVAPGAFLPAAERFHIATRIDHWVVRRVVDWM
                     AHQRDSLAHVDVLAVNLSGQSISDPAFLRLVNEIVDGARFDARKLCFEVTETAAITNM
                     ADATAFVDAMRERGIRFALDDFGAGASGFSYLKTLKVDYLKIDGQFIRDLQADPIDRA
                     TVRCFRDVAATLGTRTIAEFVENEATATLLREIGIDYGQGYLYHSPEPIDAVVRAPWA
                     TLAS"
     gene            2321..3385
                     /locus_tag="VF453_07845"
     CDS             2321..3385
                     /locus_tag="VF453_07845"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF021205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX7687602.1"
                     /translation="MSVSFGSRGARRVARGCLLAVALAAANGAAFAEGCKYKRIASLP
                     YRWDHVRMMVDASVGGHPVRAQVDTGAEPILMFEDVAERLQLRLAHLDGEAYGVGGRS
                     QTYLTQLRDLRLGRFAWDRAHAVILPRAASEIEEADSSELLVGSSLLLQHDMEIDADV
                     LRFYDPEGCGDAPLGYWADDVPWVPMLAAARGDFGVEVLVHVDGVPVRAAIDTGSPYS
                     LLDLSIARRLGFDPARAGPADESQGFGRHSMRSWLGDFGAIAIGPEVVHRPTLRVADM
                     WGHVVSDAQSMDARRVVDDDWPQMLLGADFVRTHRLLISMDQRRVYFSYVGGQPFVRP
                     AASPTPAPPSAAARSTGAPG"
     gene            3382..>3993
                     /locus_tag="VF453_07850"
     CDS             3382..>3993
                     /locus_tag="VF453_07850"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX7687603.1"
                     /translation="MKIHRAAVAAALAAGASVAAAAGCEYRPLGTIPLHWEGLRPTIA
                     GSINGQAVPMVLDTGALNTLVSGRVAARAGLQQQPVEVEQVGFGGVTAARGARAAEIS
                     FGAFRWRDVGVIVPAQAADDLPDVLVSAGFLFQHDLEFDAGRISFLEPGDCEEEHPAG
                     WAAGAPWLPMQAVGPRDPRVTVPVVVDGVALRALVDSGSPLSMI"
BASE COUNT          529 a         1437 c         1460 g          567 t
ORIGIN      
        1 tcgcgcactg gatcgagtgg cagcatcgcg ccgccggcgg cgacgtcggc gccgacgccg
       61 gcgcgatcca ctccgtcggc cgcgacatca ccgaccgcaa gctgctcgag cgcgagctcc
      121 tgagcagcga gcagcgctac cgctcgctct tcgaccacat gcaggcgggc ttcgcgctgc
      181 acgaggtcgt gctcgacgac gcgggccgcg tggtcgacgt ggtgtacctc gctgtcaacg
      241 cgccgcacgc gcgcatgatg ggcttcgacc ccgagcgcat gaccggcgcg cggctgtccg
      301 agctgctgcc ggcgggcgcc caggcggcgg cgagcgagtg gatcgcgatc ttcagccgcg
      361 tcgcgctcga cggcgagacg atctcgttcg aacgccgcgc ctacgacccg ccgcgctggt
      421 tctcggtcgt cgcctaccgg ccggcgccgg gacagttcgc gaccatcgtc gaggacatca
      481 gcgagcgcaa gcgcgcgcag gaggagatgc aggcgcagca cgaacagctg cgcgtgacgc
      541 tgcactcgat cggcgacgcc gtcatcacga cagacaacgc gggccgcgtg cagtacctca
      601 acccggtcgc cgagcgcctg accggctggc gcgacgccga tgcccgcgac cgcgcgctcg
      661 aggccgtgta ccaccctgtc gacgaggtct cgcgcgagcc gttgcccaac ccggtcgcgg
      721 cctgcctggc cggcacgccc gcgcgggcgg acgccgacga tgcggggccg ggtgcggagg
      781 cgccgcacgc ggtgctggcg agccgccacg gcagcgaagc gggcatcgag cagacggtcg
      841 cgccgatccg ggcggccgac ggcagcgtgc tcggcgcggt gctggtcttc cgcgacgtca
      901 ccgaccgccg ccgcatggcg cgcgagatga gctaccgcgc gacgcacgac gcgctcaccg
      961 ggctcgtcaa ccgcagcgac ttcgagtcgc gcctgcacca tgcgctgcgc ctcgctcacg
     1021 ccgacggcag cgtgcacgcg ctgatgtacg tcgacctcga ccagttcaag ctcgtcaacg
     1081 acgcctgcgg gcacgccgcc ggcgaccagc tgctgcgcca ggtcgcggcg ctgatcaagc
     1141 agtgcgtgcg ctcgcgcgac acgctcgcgc gcctgggcgg cgacgagttc ggcgtgatcc
     1201 tgcagttctg ttcgatcgag caggggcagc gcgtcgcgca gcagatctgc gagcgcatgg
     1261 acgagttccg cttcgcccac ggcgagcggc gcttccgcgt cggcgcgagc gtgggcctcg
     1321 tgccgctgga cagccggtgg ccgacggccg cgagcgcgat gcaggcggcc gacgcggcct
     1381 gctacgcggc caaggaggcc ggccgcaacc gcgtgcacgc gtggttcgac gccgacgaga
     1441 gcctgcgcct gcgcagcggc gagatgcagt gggtcgcgcg cctggagcag gcggtcgacg
     1501 aggaccgctt cgagctgttc gcgcagcgca tccagccgat ccgcgccgcg agctcgggcc
     1561 tgcattgcga ggtgctgctg cgcctgcgcg gcccggacgg gcggctcgtc gcgccgggcg
     1621 cgttcctgcc ggcggccgag cgcttccaca tcgccacgcg catcgatcac tgggtcgtgc
     1681 gccgcgtcgt cgactggatg gcccaccagc gcgattcgct cgcccacgtc gacgtgctgg
     1741 cggtcaacct gtcggggcag tcgatcagcg atccggcctt cctgcgcctg gtcaacgaga
     1801 tcgtcgacgg cgcgcgcttc gacgcccgca agctgtgctt cgaggtcacc gagaccgcgg
     1861 ccatcaccaa tatggccgac gcgacggcct tcgtcgacgc gatgcgcgag cgcggcatcc
     1921 gcttcgcgct cgacgacttc ggcgccggcg cgtcggggtt cagctacctg aagacgctca
     1981 aggtcgacta cctgaagatc gacggccagt tcatccgcga cctccaggcg gacccgatcg
     2041 accgcgcgac cgtgcgctgc ttccgggacg tggccgccac gctgggcacg cgaaccatcg
     2101 ccgagttcgt cgagaacgag gccaccgcga cgctgctgcg cgagatcggg atcgattacg
     2161 gtcaggggta tctgtaccac agcccggagc cgatcgacgc ggtggtgcgc gcgccgtggg
     2221 ccacgcttgc gagctgagcg cggcgcccgc cgcgtagaat cgcgcgccgc cgtaccgctc
     2281 gcagaaagcg ggggcgagcg atgtcgaaag ggggagggtc atgtccgtat cgttcggttc
     2341 gcgcggtgca cgtcgcgtcg cccgaggctg cctgctcgcg gtcgcgctgg ccgcggccaa
     2401 tggggcggcg tttgccgagg gctgcaagta caagcgcatc gcctcgttgc cgtaccggtg
     2461 ggaccacgtg cgcatgatgg tcgacgccag cgtcggcggg catcccgtgc gcgcgcaggt
     2521 cgacaccggc gccgagccga tcctgatgtt cgaggacgtc gccgaacgcc tgcagctgcg
     2581 gctggcgcat ctcgacggcg aggcctatgg tgtcggcggg cgctcccaga cctacctgac
     2641 gcaactgcgg gacctgcgac taggtcgctt cgcctgggat cgcgcccacg ccgtgatcct
     2701 gcctcgcgcc gccagcgaaa tcgaggaggc cgattcgtcc gagctcctgg tcggcagcag
     2761 cctgctgctg cagcacgaca tggagatcga cgccgacgtc ctgcgcttct atgaccccga
     2821 aggctgcggc gatgccccgc tcggttactg ggcggacgac gttccgtggg tgcccatgct
     2881 ggcggcagcg cgcggggact tcggcgtcga ggttctcgtg cacgtggacg gcgtgccggt
     2941 gcgcgcggcc atcgataccg gatccccgta ttcgctgctg gacctgtcca tcgcgcgccg
     3001 gctcggcttc gatcccgcgc gcgcgggacc ggcggacgaa agccaaggct tcggcaggca
     3061 cagcatgcgt tcctggttgg gcgacttcgg cgcgatcgcg atcggccccg aggtcgtgca
     3121 ccgcccgacg ttgcgcgtcg ccgacatgtg gggccacgtc gtaagtgacg cgcagtcgat
     3181 ggatgcacgc cgcgtcgtcg acgacgactg gccgcagatg ctgctcgggg ccgacttcgt
     3241 gcgtacgcac aggctgttga tctcgatgga ccagcgccgt gtctatttct cgtacgtcgg
     3301 aggccagccg ttcgtgcggc cagcggcatc gccgacgccc gcgccgccat ccgccgccgc
     3361 ccgctcgacg ggcgcaccgg gatgaagatc caccgcgccg cggtggccgc cgcgttggcg
     3421 gcgggcgctt cggtcgccgc ggccgccggc tgcgagtacc gccccctcgg caccatcccg
     3481 ctgcactggg agggcctgcg cccgaccatc gccggctcga tcaacggcca ggcggtgccg
     3541 atggtgctgg acaccggtgc gctcaacacg ctcgtctcgg gccgcgtcgc ggcgcgcgcc
     3601 gggctccagc agcagccggt ggaggtcgag caggtcggct tcggcggggt caccgcggcg
     3661 cgcggcgccc gcgcggcgga gatctcgttc ggcgcgttcc gctggcgcga cgtcggcgtc
     3721 atcgtgcccg cgcaggcggc agacgacctg cccgacgtgc tcgtgagcgc gggcttcctg
     3781 ttccagcacg acctcgagtt cgacgccggc cggatctcgt tcctcgagcc cggcgactgc
     3841 gaggaggagc atccggccgg ctgggccgcg ggcgcgccgt ggctgccgat gcaggccgtg
     3901 ggcccgcgcg acccgcgcgt gacggtgccg gtggtggtcg acggcgtggc gctgcgcgcg
     3961 ctggtggaca gcggctcgcc gctgtcgatg atc
//
LOCUS       DASVLN010000323         4897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_285668, whole genome shotgun sequence.
ACCESSION   DASVLN010000323 DASVLN010000000
VERSION     DASVLN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285668"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VF453_07855"
     CDS             complement(<1..494)
                     /locus_tag="VF453_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEX7687604.1"
                     /translation="MKRPAILPDNFTLMLVATVALASFAPARGLAAVGFDHLTTFAIG
                     LLFFLHGAKLSRDAIRAGIGHWRLHLLVFAFTFGLFPVLGLALRPVLEPLVTPALYAG
                     ILFLCVLPATVQSAIAFTSIARGNLPAAVCSASASTLLGVFVTPLLAGLVVMPQVPAS
                     ALAGG"
     gene            564..1457
                     /locus_tag="VF453_07860"
     CDS             564..1457
                     /locus_tag="VF453_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7687605.1"
                     /translation="MNVSLRQLRVFRSVAETLNFSRTGEAIGLTQPAVSRAIHELEAA
                     LGLRLLDRTTREVALTEAGRALSVRLVRALDELDATLADVAALADADGGKVRVASAPT
                     LSAHLMPQCIAACAREAPRVRFVLLDRIQQDVLESVRSGEVDFGVVVEPSTTDDLLCE
                     DILHDPFVLVAPARHPLARGGAGVKWKSLDGAALVLTDHASGSRRFIDAALARHGATC
                     RVEQELGHPTTVFRMVEAGIGLSVLPALAVPPGGVRGLAVRPLLPQVRRTVMLVRLAH
                     RALSPVAQRVWELVGRTARGG"
     gene            complement(1513..1887)
                     /locus_tag="VF453_07865"
     CDS             complement(1513..1887)
                     /locus_tag="VF453_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7687606.1"
                     /translation="MSNAFVWVDIPVRDLDRAIAFYGAVFGRPVEKQSAPGFTFGLFP
                     HERDEVGGCLFVPAGEDSQPSMGGPLAYLDATGRLDAAVAAALEHGGRVVQAKHSMGP
                     HGWRAIVADTEGNRIALYSPTE"
     gene            2070..2573
                     /locus_tag="VF453_07870"
     CDS             2070..2573
                     /locus_tag="VF453_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019256122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7687607.1"
                     /translation="MTASAADLSAAALIDRRIAELGGWRGRALARVRKLIHEADAGIV
                     EEWKWQNPVWSRDGIVCTGEAYKAAVKLTFPKGASLEDPAGLFNSSLEGAVRRAIDIR
                     EGDEPDAAALKALLRAAIAANAAGKAKPGATKAAARPSAKAAAKTAPGKTAARPAAKR
                     ASGKSAA"
     gene            complement(2788..4602)
                     /locus_tag="VF453_07875"
     CDS             complement(2788..4602)
                     /locus_tag="VF453_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7687608.1"
                     /translation="MPPTSAETSLISEPPEIPDKCSPIDPALVDTNAGEVPADRPHLR
                     SWPKRLPRTVVPPETSLWANVEVAVRRYPDKAATLFFGRSMSYARLLADAEALAGWLQ
                     ARGVRRGDRVALFMQNCPQYLVAYHAILRADAVVVPVNAMNKAEEFGHYIVDPGTRVV
                     ICGAELAGIVASANAALPPSQRAACVLAARYADELPEDFAPDLVPPPAMRDWLLADPP
                     LPPGTVRWRDALAQRLVPGPTVNGPDDLALLPYTSGTTGLPKGCMRTHRTLMHNVVGS
                     GLWGSSGAESVVLGVVPMFHITGMLYGVIGPMFNGATVVVMPRWERELAGRLISRHRV
                     THWTCIPTMIIDLFASPNYREFELSSLRYLSGGGAAMPQAVAQRLIDEFGLQFCEGYG
                     LTETAAPSHANPAERPKLQCLGIPFFGVDSRVVDPVTLREVPPGEVGEIVTRGPMVFR
                     GYWKNPEATAAAFVEVDGRRFFRTGDLGHVDEDGYFFITDRLKRMINASGFKVWPSEV
                     ELLLYRHPAVQEACVISARDAYRGETVKALVVLRAQARGSVEPREIVAWAHEHMAAYK
                     APRIVEFVDALPKSGAGKVLWRVLQDREDAGVAGAREG"
BASE COUNT          693 a         1775 c         1799 g          630 t
ORIGIN      
        1 ccgcccgcga gcgccgacgc cggcacctgc ggcatcacga ccagtcccgc cagcagcggt
       61 gtcacgaaca cgcccagcag cgtcgacgcc gacgcgctgc acaccgcagc cggcaagttg
      121 ccacgcgcga tcgacgtgaa cgcgatggcg gactgcaccg tcgccggcag cacgcacagg
      181 aacaggatgc ccgcgtacag cgccggcgtc acgagcggct ccagcaccgg acgcagcgcg
      241 aggccgagca cgggaaacag gccgaacgtg aacgcgaaca cgagcaggtg cagccgccag
      301 tggccgatgc cggcgcggat cgcgtcgcgc gagagcttgg cgccgtgcag gaagaacagc
      361 aggccgatgg cgaaggtcgt gagatggtcg aagccgacgg cggccaggcc gcgcgcgggc
      421 gcgaagctgg cgagcgcgac ggtggccacc agcatgagcg tgaagttgtc gggcaggatc
      481 gcggggcgtt tcatgccctc attggagcgc gtcggcctcc aaaagagaaa tcgatttgtc
      541 ggatggattc ataattcaaa atcatgaatg tgagcctgcg ccagctgcgc gtcttccgct
      601 ccgtcgcgga gacgctgaac ttcagccgca ccggcgaggc gatcggcctc acccagcccg
      661 cggtcagccg tgccatccac gagctggagg cggcgctcgg cctgcgcctg ctcgaccgca
      721 ccacgcgcga ggtcgcgctc accgaggccg ggcgcgcgct gtcggtgcgg ctcgtgcggg
      781 cgctggacga gctcgacgcc acgctggccg atgtcgcggc gctggcggac gccgatggcg
      841 gcaaggtgcg cgtcgcgagc gcgcccacgc tgtccgccca cctgatgccg cagtgcatcg
      901 ccgcgtgcgc gcgcgaggcg ccgcgggtgc gcttcgtgct gctcgaccgc atccagcagg
      961 acgtgctcga gagcgtgcgc agcggcgagg tcgacttcgg cgtcgtcgtc gagccctcga
     1021 ccaccgacga cctgctgtgc gaggacatcc tgcacgaccc gttcgtgctc gtcgcgccgg
     1081 cccgccatcc gctcgcgcgc ggcggggccg gcgtcaagtg gaagtcgctc gacggcgcgg
     1141 cgctggtgct gaccgaccac gcctccggca gccggcgctt catcgacgcg gccctcgccc
     1201 gccacggcgc cacgtgccgc gtcgagcagg agctggggca tccgaccacc gtcttccgca
     1261 tggtcgaggc cggcatcggg ctcagcgtgc tgccggcgct ggcggtgccg cccggcggcg
     1321 tgcgcggact ggccgtgcgg ccgctgctgc cccaggtgcg gcgcacggtg atgttggtgc
     1381 gactcgccca ccgggcgctg tcgccggtgg cgcagcgggt gtgggaactc gtcggccgca
     1441 ccgcgcgcgg cggctgaggc cgcgtcccgg acagcgcgcg acgccttcag ccgcgggaac
     1501 gacgcgccgc gctcattcgg tcggcgagta cagggcgatc cggttgccct ccgtgtcggc
     1561 gacgatcgcg cgccagccgt gcggccccat cgaatgcttg gcctgcacga cgcgaccgcc
     1621 gtgctcgagc gccgcggcga cggcggcgtc cagcctcccg gtggcgtcga ggtaggccag
     1681 cgggccgccc atcgacggct gcgaatcctc gcccgccggc acgaacaggc agccgcccac
     1741 ctcgtcgcgc tcgtggggga acaggccgaa cgtgaagccg ggcgcggatt gcttctcgac
     1801 cggacggccg aacaccgcgc cgtagaacgc gatggcgcga tcgaggtcgc gcacgggaat
     1861 gtcgacccag acgaaggcgt tgctcatggg aatctcctga catgtcgggg aagccgcgcg
     1921 cgatgttcgc acgtccggcg cacccacacc gcaccggccg ggccgcggcc cggccgcctc
     1981 ttcgtgcggt tcgtcgccgg ggcgctttct ccgcgccgcg atccgcgcta cgctgcccgc
     2041 tccgacatcc cggcccacgc cctcgcccca tgaccgcttc cgccgccgac ctgtccgccg
     2101 ccgccctcat cgaccgccgc atcgccgaac tcggaggctg gcgcggccgg gcgctcgcgc
     2161 gcgtgcgcaa gctgatccac gaggccgacg ccgggatcgt cgaggaatgg aagtggcaga
     2221 acccggtctg gtcgcgcgac gggatcgtct gcaccggcga ggcctacaag gccgcggtga
     2281 agctcacctt ccccaagggc gcgtcgctgg aggatccggc cggcctgttc aactcgagcc
     2341 tcgagggcgc ggtgcggcgc gcgatcgaca tccgcgaagg cgacgagccc gacgccgcgg
     2401 cgctgaaggc gctgttgcgc gccgccatcg cggcgaacgc cgcgggcaag gccaagccgg
     2461 gggccaccaa ggccgcggcc cgtccctccg caaaggcggc cgccaagacg gcgcccggga
     2521 agaccgccgc ccggcccgcc gcgaaacggg cgagcggcaa gagcgccgcc tgacggccca
     2581 cggatgcgcg cggccagcgt cgcgccggcg ccgtgtgccg aacgcctggg gcgaggcggc
     2641 gcaccttcgc gatgctgacg ggagtctccc tcccggaggg aggctggaag gtgcggtcga
     2701 acatgctgga gcgccctctc gtagggggct cgggggagcc gtgcattcaa ccgctaggag
     2761 tttgcgcggc ttctgccgcg cggactccta gccctcgcgc gcgcccgcga ccccggcgtc
     2821 ctcgcgatcc tgcagcaccc gccacagcac cttgcccgcg cccgacttcg gcagcgcatc
     2881 cacgaactcg acgatgcgcg gcgccttgta ggcggccatg tgctcgtgcg cccaggcgac
     2941 gatctcgcgc ggctcgacgc tgccccgcgc ctgcgcgcgc agcacgacca gggccttcac
     3001 cgtctcgccg cggtacgcgt cgcgggccga gatcacgcag gcttcctgca cggcgggatg
     3061 gcggtacagc agcagctcga cctccgacgg ccagaccttg aagccgctcg cgttgatcat
     3121 gcgcttcagg cgatcggtga tgaagaaata gccatcctcg tcgacgtggc ccaggtcgcc
     3181 ggtgcggaag aagcgccggc cgtcgacctc gacgaaggcc gcggcggtcg cttccgggtt
     3241 cttccagtag ccccggaaga ccatcgggcc gcgggtgacg atctcgccga cctcgccggg
     3301 cggcacctcg cgcagcgtca ccggatcgac gacccgcgaa tcgacgccga agaacgggat
     3361 gcccaggcac tgcagcttgg gacgctcggc cggattggcg tggctcggcg cggcggtctc
     3421 ggtgaggccg tagccttcgc agaactgcag gccgaactcg tcgatcagcc gctgcgcgac
     3481 ggcctgcggc atcgccgcgc ccccgcccga caggtagcgc aggctggaga gctcgaactc
     3541 gcggtagttc gggctcgcga acaggtcgat gatcatcgtc gggatgcacg tccagtgcgt
     3601 cacgcggtgg cgcgagatca ggcggccggc gagctcgcgc tcccagcgcg gcatcacgac
     3661 gaccgtcgcg ccgttgaaca tcggtccgat gacgccgtac agcatgcccg tgatgtggaa
     3721 catgggcacc acgcccagca cgacgctctc ggcgccgctg ctgccccaca ggccgctgcc
     3781 gacgacgttg tgcatcagcg tgcggtgggt gcgcatgcag cccttgggca ggccggtcgt
     3841 gccggaggtg tacggcagga gcgcgaggtc gtcggggccg ttgaccgtcg gaccgggcac
     3901 gagccgctgc gcgagcgcgt cgcgccagcg cacggtgccc ggaggcagcg gcgggtcggc
     3961 gagcagccag tcgcgcatgg ccggcggcgg gacgaggtcg ggcgcgaagt cctcgggcag
     4021 ctcgtccgcg tagcgcgccg cgagcacgca ggccgcgcgc tgcgagggcg gcagcgcggc
     4081 gttggcgctc gcgacgatgc ccgcgagctc ggcgccgcag atcacgacgc gcgtgcccgg
     4141 atcgacgatg tagtggccga actcctcggc cttgttcatc gcgttgaccg gcacgacgac
     4201 cgcgtcggcg cgcaggatcg cgtggtacgc gaccagatac tgcgggcagt tctgcatgaa
     4261 cagggcaacg cggtcgccgc gccgcacgcc tcgcgcctgc agccagccgg caagcgcctc
     4321 ggcatcggcc agcagccgcg cgtagctcat cgagcggccg aagaacagcg tcgcggcctt
     4381 gtcgggatag cgccgcaccg cgacctcgac gttggcccac agcgaggtct cgggaggcac
     4441 gaccgtgcgc ggcaggcgct tcggccagga gcgcaggtgc gggcggtcgg ccggcacctc
     4501 gccggcgttg gtgtcgacga gcgcgggatc gatcggggaa cacttgtcgg gtatctcggg
     4561 cggctcggaa atgagggatg tctcggcgga cgtcgggggc attgcggatc ctttgcgcgc
     4621 gacggcatct gcatgcgtcg tccgccagcg tactgccgtc tgtcggcctg cggccctagt
     4681 gcgagcacgg agcccggctg tcgcccgcgg gacagcccct gcgagcgtcc gccgcggccg
     4741 gatgccggtc gcaacgcggc cacgcgtcgt cgagcccgca tcgggccgca agcgggccgg
     4801 gccgcgtccc gccgcccggc tcggagtacg tttgcggctc ttccggggtc gttcgcggct
     4861 tgtccgtgcc tcgcccgggg gcgcgatcgc cggtcgc
//
LOCUS       DASVLN010000324         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_69919, whole genome shotgun sequence.
ACCESSION   DASVLN010000324 DASVLN010000000
VERSION     DASVLN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69919"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF453_07880"
     CDS             complement(<1..197)
                     /locus_tag="VF453_07880"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX7687609.1"
                     /translation="MRHGSVDYFAADGTPVPPYAVPLNATGRAQADAAGALFAQAGVA
                     FDRVIASGLARTVETATRVLAA"
     gene            complement(307..1086)
                     /locus_tag="VF453_07885"
     CDS             complement(307..1086)
                     /locus_tag="VF453_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7687610.1"
                     /translation="MPSELLTSREGSTLILTLSDPATRNTLSPQVYAAGVEALNMAES
                     DDSVRTVIVRGDGEHFCAGGDLNRIITLRQAGPEAQAESVARFHLLIDSLRAFPKPVI
                     AAVEGFAAGGGMSLALACDLVVAASDAKFVMSYARVGLTPDGGGSWHLARLLPRALAL
                     EMIWLAAPVTAQRLHELGLVNRVTDKGQALAEALGLAAKLGAMAPNAVAGAKELVARA
                     PSSSLAEHLEAERAQFVANLFHDNGGEGLKAFFEKRPAAFR"
     gene            1267..>1650
                     /locus_tag="VF453_07890"
     CDS             1267..>1650
                     /locus_tag="VF453_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687611.1"
                     /translation="MKKTTTAQIVVAATALASQQACALGFGRVVSDAVLGQPLSATVP
                     VHVDPGERFGVDCVSVDVYSGESRLPPSQVRAAVDPGNSDTDWRVRISTTVAIDEPIV
                     EVALNAGCERRFLRRFTVFANPAAAE"
BASE COUNT          233 a          557 c          627 g          233 t
ORIGIN      
        1 gccgcgagca cgcgcgtcgc ggtctcgacg gtgcgcgcga gcccgctcgc gatcacgcgg
       61 tcgaacgcga ccccggcctg cgcgaacagg gcgcccgcgg cgtcggcctg cgcgcgcccg
      121 gtcgcgttga gcggcaccgc atacggtggc accggcgtgc cgtcggcggc gaagtagtcg
      181 accgagccgt ggcgcatcag gtagaggcgg cggcgggcag ggggggcgaa cagggcggcg
      241 ggatggggtc ggtcggcgtt cacggcggcg ggcaatgcgg gcgaggaggc gggccgccga
      301 gggcggtcag cggaaggcgg cagggcgctt ctcgaagaag gccttgaggc cctcgccgcc
      361 gttgtcgtgg aacaggttgg cgacgaactg ggcgcgttcg gcctcgaggt gctcggccag
      421 cgacgacgag ggcgcgcgcg cgaccagctc cttggcgccg gccaccgcgt tgggcgccat
      481 cgcgcccagc ttcgcggcga ggcccagagc ttcggcgagc gcctggccct tgtctgtcac
      541 gcggttgaca aggcccagct cgtgcaggcg ctgcgccgtc acgggggcgg cgagccagat
      601 catctccagc gccagcgcgc gcggcagcag gcgcgcgagg tgccacgagc cgccgccgtc
      661 gggcgtcagg ccgacgcgcg cgtacgacat gacgaacttc gcgtcggacg ccgcgacgac
      721 gaggtcgcag gcgagcgcga gcgacatccc gccgccggcc gcgaagccct cgaccgccgc
      781 gatcaccggc ttcgggaacg cgcgcagcga atcgatcagc aggtggaagc gcgcgaccga
      841 ttccgcctgc gcctcgggcc cggcctggcg cagggtgatg atgcggttca ggtcgccgcc
      901 ggcgcagaag tgctcgccgt cgccgcgcac gatcaccgtg cgcaccgagt cgtccgactc
      961 ggccatgttc aacgcctcga cgccggccgc gtagacctgc ggcgacagcg tgttgcgcgt
     1021 ggcggggtcg ctgagcgtga ggatcagcgt cgacccctcg cgggaggtca ggagttcact
     1081 gggcatgtgg ggcctcggaa ggttttcgaa atatcgggcc ggcgtattgc cggcaaagga
     1141 gatattcggg acgcattgtg cgtccaagct aattcacggt cgcgtcgcca atacgggccg
     1201 ggcttccccg gccggccgcg gcgggcaatc gtgctagagt cgtcggcgct accgccttcc
     1261 acgggaatga agaaaaccac caccgcgcaa atcgtcgtcg ccgcgaccgc cctggcttcc
     1321 cagcaggcct gcgcgctcgg cttcggtcgc gtggtgtcgg atgcggtact cggccagccc
     1381 ctttccgcga cggtgccggt gcacgtggat ccgggcgagc gattcggcgt cgattgcgtt
     1441 tcggtcgacg tttattcggg ggaatcccgg ctcccgccga gccaggttcg ggccgcggtg
     1501 gatccgggga actccgatac cgattggcgc gtgagaatct cgacgaccgt cgcgatcgac
     1561 gagcccatcg tcgaggtggc gctcaacgcg ggttgcgagc ggcgtttcct gcgccgcttc
     1621 acggtattcg ccaatcccgc ggccgccgag
//
LOCUS       DASVLN010000325         4583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_391730, whole genome shotgun sequence.
ACCESSION   DASVLN010000325 DASVLN010000000
VERSION     DASVLN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391730"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF453_07895"
     CDS             <1..362
                     /locus_tag="VF453_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7687612.1"
                     /translation="VGAPALAALREWLAHRPELVGADAEPALFVSRLGRRLSTNQLRA
                     RLKAQSARAGVATPVHPHMLRHSYASHLLQSSGDLRGVQELLGHASIATTQVYTRLDF
                     QHLAQVYDAAHPRAKRK"
     gene            462..935
                     /locus_tag="VF453_07900"
     CDS             462..935
                     /locus_tag="VF453_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687613.1"
                     /translation="MTRLTKRLAAIALVAGASAALPCFAESSVASSLSDSLSRSSASI
                     SDSITGSSHASSPDNKQVKGDYRVTDVAEVADRPGMVRLALQPMAAGTTADDEIFLTL
                     PREAAADGHVARDAVVTAMQRPYGIEFASGQPHAAFFLALADDWARDLKAAPLTL"
     gene            932..1885
                     /locus_tag="VF453_07905"
     CDS             932..1885
                     /locus_tag="VF453_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HEX7687614.1"
                     /translation="MKAGLGRIRALGAALALLATSCGAASLRACEQRAEFSAGQKDVI
                     LRFAAVVKEQLAASGSPVALVARSGMDLRRLQVRYSHEGVALDGDGSGAWKVRELYYA
                     CDERRPRIYDEGLAGFLLGTDDPGTGWVSIVLLPPAPAQALARTATDKHHALGVLGAT
                     YSANAYPFSTRFQNCNQWVMELLADAWDADDDAPPVDASAPGPADSTAARARAQRWLR
                     AQGYLPTVFTVSARPMTWLADIVPWMSNADHPPEELAHGRYDVSMPSSIETFVQATVP
                     GATRLEVCHAGRRVVVHRGWDAIADGCVAGPGDETIALGTH"
     gene            2082..2453
                     /locus_tag="VF453_07910"
     CDS             2082..2453
                     /locus_tag="VF453_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7687615.1"
                     /translation="MIEHLSLGTHGYGDAVAFFQRALAPLDISLQRDTGQEAAFGTAS
                     RWSFFLYPVPAEDGVVAARMHVAFGAPSRAAVEQVHAAALAAGASDVFGPRERPDISP
                     TYYGAMFKSADGHAIEVLTNA"
     gene            2514..2990
                     /locus_tag="VF453_07915"
     CDS             2514..2990
                     /locus_tag="VF453_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7687616.1"
                     /translation="MELDRFDRQLLRLVQEDSARTTEQLATQVALSPSAIQRRLRRLR
                     EAGVIAREIAVVDPRRVGRSTMFLASVQVESERPERMGRLREWLASEELVQQAFYVTG
                     EADFVLVVCAPDTETYDAFMGRLVDANPNVKRFTTNVALNVLKRGLTVPIHDGAQD"
     gene            complement(2993..4279)
                     /locus_tag="VF453_07920"
     CDS             complement(2993..4279)
                     /locus_tag="VF453_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEX7687617.1"
                     /translation="MLPATPTPDFRSPAFLRAHVADTMAFYEGRCVDASGGFFQFFKD
                     DGTVYDARTRHLVSSTRYVFTQAMGFRHFQNPAWQRAARHALSFVEAAHAQPNGGYAW
                     VLDWHDGRATVTDDTNHCYGLAFVLLAHAHALLAGHLDAADGLERTWQLMERRFWRPA
                     DSLYADEATPDWTVAPYRGQNANMHGCEAMMAAYRATGDAKYLDRAVTLADAITVRQA
                     ARSADLPGVAPDCAALVWEHFREDWAIDPDYNRGDRTNIFRPWGFQTGHQTEWTKLLL
                     QLDHLCATAGLAPDPTRLDRARRLFDVAMRHGWDDAHGGLVYGFAPDGSLYDGDKYHW
                     VQAESLAAAAWLAVALENAGAPAADVAHYWGWYDRLWAYAWAHFVDHRYGAWYRILAP
                     DNAKVTDEKSPAGKVDYHDMGACYDVLAALDARAAR"
BASE COUNT          592 a         1711 c         1662 g          618 t
ORIGIN      
        1 cggtcggcgc accggcgctc gcggcgctgc gcgagtggct ggcgcatcga ccggagctcg
       61 tgggcgcgga cgcggagccc gccctgttcg tgagccgcct cggccgccgc ctgtcgacca
      121 accagctgcg cgcgcggctg aaggcgcagt cggcgcgcgc cggcgtcgcg acgcccgtgc
      181 atccgcacat gctgcgccac agctacgcct cgcacctgct gcagtccagc ggcgacctgc
      241 gcggcgtgca ggagctgctc gggcacgcca gcatcgcaac gacgcaggtc tacacgcggc
      301 tcgacttcca gcacctggcg caggtctacg acgcggcgca tccgcgtgcc aagcggaagt
      361 agcgcccggc gcgcgggact gtcaccgcac gtttccgcgc gaccgcggcg cgagattcgc
      421 tggcacactt cgcctgttcc gctactgtcc tggagtcccc gatgacccgc ctgaccaagc
      481 gcctcgccgc gatcgccctc gtcgccggtg cgtccgccgc cctgccctgc ttcgccgaga
      541 gctccgtggc ctcgtcgctg tcggacagcc tgtcgcgttc gtcggccagc atctcggact
      601 cgatcaccgg ctccagccat gcgagctcgc cggacaacaa gcaggtcaag ggcgactaca
      661 gggtgaccga cgtcgcggag gtcgccgacc gtcccggcat ggtgcgcctc gcgctgcagc
      721 cgatggccgc ggggaccacg gcggacgacg agatcttcct cacgctcccg cgcgaggccg
      781 cggccgacgg gcacgtcgcg cgcgacgccg tcgtgaccgc gatgcagcgc ccgtacggca
      841 tcgagttcgc cagcggccag ccgcacgcgg ccttcttcct cgcgctcgcc gacgactggg
      901 cgcgcgacct gaaggcggcg ccgctgacgc tttgaaggcg ggcctgggcc ggattcgagc
      961 gctcggcgcg gcgctcgcgc tgctcgcgac ctcctgcggc gcggcctcgc tgcgggcctg
     1021 cgagcagcgg gccgaattca gcgccgggca gaaggacgtg attctgcgct tcgcggccgt
     1081 cgtcaaggag cagctcgcgg cgagcggctc gccggtggcg ctggtcgcgc gctccggcat
     1141 ggacctgcgc cggctgcagg tgcgctattc gcacgaaggc gtcgcgctcg acggcgacgg
     1201 cagcggcgcc tggaaggtgc gcgagctgta ctacgcctgc gacgagcgcc gcccacgcat
     1261 ctacgacgag gggctcgccg gcttcctgct cggcaccgac gatccgggca cgggctgggt
     1321 ctcgatcgtg ctgctgccgc ccgcgccggc gcaggcgctc gcccgcaccg ccaccgacaa
     1381 gcaccacgcg ctgggcgtgc tcggcgccac ctacagcgcg aacgcgtacc cgttcagcac
     1441 gcgtttccag aactgcaacc agtgggtgat ggagctgctc gccgacgcgt gggacgccga
     1501 cgacgacgcg ccgccggtcg acgcctccgc gcccgggccg gccgattcga ccgccgcccg
     1561 cgcgcgcgcc cagcgctggc tgcgcgcgca gggctacctg ccgaccgtct tcacggtgag
     1621 cgcacggccg atgacctggc tcgccgacat cgtgccgtgg atgtcgaacg ccgaccaccc
     1681 gcccgaggag ctcgcgcatg gccgctacga cgtgagcatg ccgtcctcga tcgagacctt
     1741 cgtgcaggcc accgtgcccg gtgcgacgcg cctcgaggtc tgccacgccg gccggcgcgt
     1801 cgtcgtccat cgcggctggg acgcgatcgc cgacggctgc gtcgcgggcc cgggcgacga
     1861 gacgatcgcg ctcgggacgc attagcgcgg atcggccgcc gcgtccgcgg attccgcgcg
     1921 cgcacgggcc ccgaaacgcg gccgggcgac gtgccgtcct ccctacgatg gacgcccgcg
     1981 ccgtcgtcgt ccggagcgcg caccgcaggc ccgtcctgta ccccggacga cctttccaac
     2041 cccttgcacg gtcccgcccc cagcgccgga cccggagcac catgatcgaa cacctctccc
     2101 tcggcaccca cggctacggc gacgcggtcg ccttcttcca gcgcgccctc gcgccgctgg
     2161 acatctccct gcagcgcgac accggccagg aggccgcctt cggcacggcc tcgcgatggt
     2221 cgttcttcct ctatcccgtg ccggcggagg acggggtcgt cgccgcccgc atgcacgtgg
     2281 cgttcggcgc gccgtcgcgc gcggcggtcg agcaggtgca cgcggcggcc ctggccgcgg
     2341 gcgcgagcga cgtcttcggc ccgcgggagc gccccgacat cagcccgacc tactacggcg
     2401 cgatgttcaa gagcgcggac ggccacgcga tcgaggtgct gaccaacgcc tgaccgcgcg
     2461 ggcccgccgc gaccgcgccc gtcgtccgcg cggcgcggct agactgcggg cccatggagc
     2521 tcgatcgatt cgaccgccag ctgctgcggc tcgtccagga ggactcggcc cgcaccacgg
     2581 agcagctggc cacgcaggtg gcgctgtcgc cgtccgcgat ccagcgccgc ctgcgccggc
     2641 tgcgcgaggc gggcgtgatc gcgcgcgaga tcgcggtcgt cgatccgcgc cgcgtcgggc
     2701 gctcgacgat gttcctcgcg tcggtgcagg tcgagagcga gcgtcccgag cggatgggcc
     2761 ggctgcgcga atggctcgcg tcggaggagc tcgtgcagca ggccttctac gtgaccggcg
     2821 aggccgactt cgtgctggtc gtctgcgcgc ccgacaccga gacctacgac gccttcatgg
     2881 gccggctggt cgacgcgaac ccgaacgtga agcgcttcac caccaacgtg gcgctgaacg
     2941 tgctcaagcg cggcctgacc gtgccgatcc acgacggcgc ccaggactga gatcagcgcg
     3001 ccgcgcgcgc gtcgagcgcc gccagcacgt cgtagcacgc gcccatgtcg tggtagtcga
     3061 ccttgccggc ggggctcttc tcgtcggtga ccttggcgtt gtccggcgcc aggatgcgat
     3121 accacgcccc gtaccggtgg tcgacgaagt gcgcccacgc gtaggcccag agacggtcgt
     3181 accagcccca gtagtgcgcg acgtccgcgg ccggcgcgcc ggcgttctcc agcgccaccg
     3241 ccagccacgc ggcggcggcc aggctctctg cctgcaccca gtggtacttg tcgccgtcgt
     3301 agagcgagcc gtcgggcgcg aagccgtaga cgaggccgcc gtgcgcgtcg tcccagccat
     3361 ggcgcatcgc gacgtcgaac agccgccgcg cccggtccag ccgggtcggg tcgggcgcga
     3421 ggccggcggt ggcgcacagg tggtcgagct gcagcagcag cttcgtccac tcggtctggt
     3481 ggccggtctg gaagccccag ggccggaaga tgttggtgcg gtcgccgcgg ttgtagtccg
     3541 ggtcgatcgc ccagtcctcg cggaaatgct cccacacgag cgccgcgcag tcgggcgcga
     3601 cgccgggcag gtcggccgag cgcgcggcct ggcgcacggt gatcgcatcc gcgagcgtga
     3661 ccgcgcggtc gaggtacttc gcatcgccgg tcgcacggta cgcggccatc atcgcctcgc
     3721 agccgtgcat gttggcgttc tggccgcggt acggggcgac cgtccagtcg ggcgtggcct
     3781 cgtcggcgta gagcgaatcg gcagggcgcc agaagcgccg ctccatcagc tgccaggtgc
     3841 gctccaggcc gtcggcggcg tccaggtggc cggccagcag cgcgtgcgcg tgggcgagca
     3901 gcacgaacgc gagcccgtag cagtggttgg tgtcgtcggt caccgtggcg cggccgtcgt
     3961 gccagtccag cacccaggcg tagccgccgt tcggctgcgc atgcgcggcc tcgacgaagg
     4021 agagcgcatg gcgggccgcg cgctgccacg ccgggttctg gaagtggcgg aagcccatcg
     4081 cctgcgtgaa cacgtagcgc gtgctgctga cgagatggcg ggtgcgggcg tcgtagacgg
     4141 tgccgtcgtc cttgaagaac tggaagaagc cgccgctcgc gtccacgcag cggccctcgt
     4201 agaaggccat cgtgtcggcg acgtgggcgc gcaggaacgc gggagagcgg aagtccgggg
     4261 tgggcgtggc gggcagcatg cggcgatgat acgaggatgg ggatccgcgc gatccggggc
     4321 ctgggcgccg gcgcgcccga gcgcctgggc ggccgttcgc gccggcttcg atgcggttcg
     4381 tcgcatcgcg ctgtcgcccg gcggcgccgg cgccaacaat gcggcgcacc tgccgcgcat
     4441 acccagcgcg gtatccagac gaatcccagg agacccgatg tcccgaccgt ccatcgcagc
     4501 cgcggggctg ctgctcgcgc tcgccctgtg cggtggcgcg caggcgcagc gcgagagcgc
     4561 gccttccgtc gccgccgcgc ccg
//
LOCUS       DASVLN010000326         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_124602, whole genome shotgun sequence.
ACCESSION   DASVLN010000326 DASVLN010000000
VERSION     DASVLN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124602"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1042
                     /gene="rpoA"
                     /locus_tag="VF453_07925"
     CDS             56..1042
                     /gene="rpoA"
                     /locus_tag="VF453_07925"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7687618.1"
                     /translation="MQTNLLKPKAINVEPLGGHRAKVTLEPFERGYGHTLGNALRRVL
                     LSSMVGYAPTEVTIAGVLHEYSTVDGVQEDVVHVMLNLKGVVFRLHNRDEVTLVLRKE
                     GEGPVTAGDIQTPHDVEIVNPEHIVAHLAQGGKLDMQIKVEKGRGYVPGNMRRFGDET
                     TKAIGRIVLDASFAPVKRVSYAVENARVEQRTDLDKLVMEIETNGAISPEEAIRSSAK
                     ILVEQLAVFAQLEGSDIASFEAPTPRAAQFDPILLRPVDELELTVRSANCLKAENIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGARLENWPPQGLDKR"
     gene            1181..1576
                     /gene="rplQ"
                     /locus_tag="VF453_07930"
     CDS             1181..1576
                     /gene="rplQ"
                     /locus_tag="VF453_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX7687619.1"
                     /translation="MRHRHGLRKLNRTSSHRQAMFRNMANSLIQHEAIKTTLPKAKEL
                     RRVVEPLITLAKEPTVANRRLAFDRLRDRDNVVKLFDVLGPLFKARPGGYTRILKMGF
                     RPGDNAPMAFVELVERSHDEEAVADTTGE"
     gene            1823..>2386
                     /locus_tag="VF453_07935"
     CDS             1823..>2386
                     /locus_tag="VF453_07935"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX7687620.1"
                     /translation="MLSIRSAALALLVLLAVALGLVAQARADEPFLDPAQAFQLSVRT
                     LPGERVELTYKIAPRYYLYRERFKFSSPEATLGEPQMPPGAKHYDKALEQEVETYHDQ
                     VVVVVPVAKASKSFTIAATHQGCAEAGLCYPPQPRTIDVTLKAFGGGVDSAAVRPVAT
                     DDGGGGGPVPGAVVTLPQDKPSGGMSVA"
BASE COUNT          413 a          864 c          763 g          346 t
ORIGIN      
        1 tccatcagcc ttatcggtgt aacgagccga gggtattgag aggaacagga catacatgca
       61 aaccaatctg ctgaaaccca aagccatcaa tgtcgagccg ctcggcgggc atcgtgccaa
      121 ggtcaccctc gagccgttcg aacgcggcta cgggcacacc ctgggcaacg ccctgcgtcg
      181 cgtgctgctg tcgtcgatgg tgggttacgc gccgaccgag gtcacgatcg caggcgtcct
      241 gcacgaatac tcgacggtcg acggcgtgca ggaagacgtc gtgcacgtca tgctgaacct
      301 gaagggcgtg gtcttccgcc tgcacaaccg tgacgaggtc acgctggtgc tgcgcaagga
      361 gggcgagggc ccggtgaccg ccggcgacat ccagacgcct cacgacgtcg agatcgtcaa
      421 ccccgagcac atcgtggccc acctggccca gggcggcaag ctcgacatgc agatcaaggt
      481 cgagaagggc cgcggctacg tgccgggcaa catgcgccgc ttcggcgacg agacgaccaa
      541 ggccatcggc cgcatcgtcc tcgacgcctc gttcgcgccg gtcaagcgcg tcagctacgc
      601 ggtcgagaac gcccgcgtcg agcagcgcac cgacctcgac aagctcgtga tggagatcga
      661 gaccaacggc gccatctcgc ccgaggaagc gatccgctcg tcggccaaga tcctggtcga
      721 gcagctcgcg gtcttcgcgc agctggaagg cagcgacatc gccagcttcg aggcgccgac
      781 gccgcgcgcc gcgcagttcg accccatcct gctgcgcccg gtcgacgagc tggaactcac
      841 ggtccgttcg gcgaactgcc tgaaggccga gaacatctac tacatcggcg acctgatcca
      901 gcgcaccgag accgagctgc tgaagacccc gaacctgggc cgcaagtcgc tcaacgagat
      961 caaggaagtc ctcgcctcgc gcggcctcac gctgggcgcg cgtctcgaga actggccgcc
     1021 gcagggcctc gacaagcgct gatccgtcgg cgcgagtcaa tccggtcgcc tgcctccggg
     1081 cggcggccgt ttccgcagcc gggcgcatgg ggcgcccgag tgccaacccc cggtacctga
     1141 tccggccggg gttcaataac gatagtcaag gaaacacgtc atgcgccatc gtcacggcct
     1201 tcgcaagctc aaccgcacct cgtcgcaccg ccaggccatg ttccgcaaca tggccaactc
     1261 gctgatccag cacgaggcca tcaagaccac cctgcccaag gccaaggaac tgcgtcgcgt
     1321 cgtcgagccg ctgatcaccc tggccaagga gccgaccgtc gcgaaccgcc gcctggcgtt
     1381 cgaccgcctg cgcgatcgcg acaacgtggt caagcttttc gacgtcctcg gcccgctgtt
     1441 caaggcgcgt ccgggcggct acacccgcat cctgaagatg ggcttccgtc cgggcgacaa
     1501 cgcgccgatg gcgttcgtcg agctggtcga gcgctcgcac gacgaggaag cggtcgcgga
     1561 cacgacgggc gagtaagccc gccgccgcgc tggtcgcggt cgcattctcc agaaagccgg
     1621 ccgctgcgcc ggctttcttc atttccgggc cgcatgcgca ggcaagcgcg gacacccgtc
     1681 gcgacgcgga accccgggcg tgctcgacgg ctcgaaccag cgccccgcgg ccgctcttcg
     1741 tcccctgccg tgtgctcccg cgccacaacc cgcgacaatg cacgattcgc cgactacccc
     1801 cgaccttcga aggcgctccc cgatgctgtc gatccgctcc gccgcgctgg cgctgctcgt
     1861 cctgctggcc gtcgcactgg gcctcgtcgc gcaggcgcgc gccgacgagc ccttcctcga
     1921 tcccgcgcag gctttccagc tgtccgtgcg gacgctgcct ggcgagcgcg tcgagctcac
     1981 ctacaagatc gcgcccaggt actacctgta ccgtgagcgc ttcaagttct cctcgcccga
     2041 ggcgacgctg ggcgaaccgc agatgccgcc cggagcgaag cactacgaca aggcgctgga
     2101 gcaggaggtc gagacctacc acgaccaggt cgtcgtcgtt gtgccggtcg cgaaggcgtc
     2161 gaaatccttc acgatcgccg cgacccacca gggctgcgcc gaggcgggcc tgtgctatcc
     2221 gccgcagccg cgcaccatcg acgtcacgct gaaggcgttc ggcggcggcg tcgacagcgc
     2281 ggccgttcgc ccggtcgcga cggacgacgg cggtggcggt gggcccgtgc ccggcgccgt
     2341 cgtcacgctg cctcaggaca agcccagcgg cggcatgagc gtggct
//
LOCUS       DASVLN010000327         3215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_338731, whole genome shotgun sequence.
ACCESSION   DASVLN010000327 DASVLN010000000
VERSION     DASVLN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338731"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VF453_07940"
     CDS             complement(<1..1056)
                     /locus_tag="VF453_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346212.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX7687621.1"
                     /translation="MMQQYLRIKAEHPDTLVFYRMGDFYEMFYDDARHAHRLLDITLT
                     TRGQSNGAPVVMAGVPVHSIESYLARLIKLGEAVALAEQVGEVGAAKGPVERKVVRVV
                     TPGTVTDTELLAERQDTVLLAVFMSRARCGLAWLALAEGRLGLGECAESELPLWLARL
                     VPAEILVSPEAAPAAVRQGTVSVVNRPAWQFDAELGVRKLQELLGVSTLAGFNAQGLS
                     RAHAAAAALISYAEHTSGGARAALGHVRSVAVEDAGDLLTLPPATLRNLEITQTLRGE
                     SAPTLLSLLDTCRTGMGSRQLRLWLTHPLRDRTAARERHDAIAVLADHGPEPLRQALR
                     GVSDVQRIAARIALRQVR"
     gene            1285..1776
                     /locus_tag="VF453_07945"
     CDS             1285..1776
                     /locus_tag="VF453_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687622.1"
                     /translation="MKLTLTQDLIYKMSLDKAPILGSKPQAFEAVEGPNYLVIDDHRE
                     SPPGFAARVGKLSTTYLVDKVVAGKRMKIHVGLARGRKGSEKPVSLADARARLGPLAD
                     RQEARRQPQIRRRADRGVGAHPRAGVRPLRGLPEEQGAAGEEELVRLAEAGAAQAQGL
                     GRP"
     gene            1664..2752
                     /locus_tag="VF453_07950"
     CDS             1664..2752
                     /locus_tag="VF453_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7687623.1"
                     /translation="MFDRYEGYLRSRAQPAKKNSFDSLRQARRKLKDWDDRKVRLISA
                     AEILDRFDLHAVAKGHRTSAEAMGRWVTTAVDKAIEREIHDAHSAGRAPSLTYNPFTI
                     LRTEERYRSNTQLEREYKAKGVRNPLSFSDTVGKYVEAAWRYRRQNPLAADFILLTML
                     WSNRRGESATFKWRDQVPDAQAPTERWIDDRLGVGHVGDAKNRGDHEFPIGPFAMQLL
                     KLRRAEQTEGQVWVFPGRNPKSEGGNYKDPTVAMATVKEMAGVKQARGHDLRRTFGAA
                     CEKLGFSDRQTKRMLGHATGSGQSVNRYTDPEWTDIADRMRRVEELILSKAPNVYNAL
                     RPAGTPALPEPDFEMAPVPERPTRRAPE"
     gene            complement(2762..>3215)
                     /locus_tag="VF453_07955"
     CDS             complement(2762..>3215)
                     /locus_tag="VF453_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687624.1"
                     /translation="TASTRDPAPLTTGTRRLRSSDSPWQTLTSLRAQFNNAVLQFGFE
                     TALLDTPHLDMLCDISSQERQDSRLTVADARGDGRLKAHAIPREFLGRVSEATIKKSI
                     AALLETSRQRIMELRSAKSTSFADGLSNGLWTQQFANCSLYLKGVEEP"
BASE COUNT          559 a         1089 c         1089 g          478 t
ORIGIN      
        1 ccgcacctgg cgcagcgcga tgcgcgcggc gatgcgctgc acgtcgctca cgccgcgcag
       61 cgcctggcgc agcggctcgg ggccgtggtc ggcaaggacg gcgatggcgt cgtggcgctc
      121 gcgcgcagcg gtgcggtcgc gcaagggatg cgtgagccac agccgcagct ggcggctgcc
      181 catgcccgtg cggcaggtgt ccagcagcga cagcagcgtc ggcgcggact cgccgcgcag
      241 cgtctgcgtg atctcgaggt tgcgcagcgt ggccggcggc agcgtgagca ggtcgcccgc
      301 atcctcgacc gccaccgagc gcacgtgccc gagcgccgcg cgcgcgccgc ccgaggtgtg
      361 ctcggcgtag ctgatcaacg ccgcggcggc cgcgtgcgcg cgcgacagtc cctgcgcgtt
      421 gaagccggcc agcgtcgaca cgcccagcag ctcctgcagc ttgcgcacgc ccagctcggc
      481 gtcgaactgc caggcgggcc ggttgaccac cgagaccgtg ccctggcgca ccgccgcggg
      541 ggcggcctcg ggcgagacca ggatctccgc cggcacgagg cgcgcgagcc acagcggcag
      601 ctcggactcc gcgcattcgc ccaggcccag ccggccctcg gccagcgcca gccacgcgag
      661 accgcatctc gcgcgcgaca tgaagacggc cagcagcacc gtgtcctggc gctcggcgag
      721 cagctcggtg tccgtcaccg tgccgggcgt caccacgcgc acgaccttgc gctccaccgg
      781 ccccttggcc gcgccgacct cgcccacctg ctccgcgagc gccaccgcct cgcccagctt
      841 gatcaggcgc gcgaggtagg actcgatcga atgcaccggc acgcccgcca tcacgaccgg
      901 cgcgccgttg ctctggccgc gcgtggtcag cgtgatgtcc agcaggcggt gcgcgtggcg
      961 cgcgtcgtcg tagaacatct cgtagaagtc ccccatgcga tagaacacga gggtgtcggg
     1021 atgctccgcc ttgatgcgca ggtactgctg catcatgggc gtgtggcctg agaagtctcc
     1081 gctctcccat gggcgggacg tcaatttttc gttttcgctc aatgccttag cctcggtctc
     1141 ttccaccaca tgttgccccg tgacgaccca tgtcagccgg agagacctgc gccaaaagag
     1201 tgccaaagaa agtttggctc agcgggatga ggcaaatttg gctcatcgcg ccgtattcgg
     1261 cggccggacc ccggaggagg ccccttgaag ctgacgctga cgcaggacct gatctacaag
     1321 atgtcgctcg acaaggcgcc gatcctgggc agcaagccgc aggcgttcga ggccgtcgag
     1381 gggcccaatt atctggtcat cgacgaccac cgcgaatcgc cgcccggatt cgcggcgcgc
     1441 gtcggcaagc tgtcgacgac gtatctggtc gacaaggtgg tcgccggcaa gcgcatgaag
     1501 atccatgtcg gcctcgcgcg cggccgcaag ggcagcgaga agccagtctc gctggccgac
     1561 gcccgcgcgc gcctgggacc tcttgcagat cgccaagaag cacggcgcca accccagatc
     1621 cgtcgacgag cagatcgagg cgtcggagct caccctcggg caggtgttcg accgctacga
     1681 gggctacctg aggagcaggg cgcagccggc gaagaagaac tcgttcgact cgctgaggca
     1741 ggcgcggcgc aagctcaagg actgggacga ccgtaaggtg cgcctgatct cggcggcgga
     1801 gatcctcgac cgcttcgacc tgcacgccgt cgccaagggc catcgaacct cggcggaggc
     1861 catgggccgc tgggtgacga ccgccgtcga caaggccatc gagcgcgaga tccacgacgc
     1921 gcactccgcc ggccgcgccc cgagcctgac ctacaacccg ttcacgattt tgcgaaccga
     1981 ggagcgctac cggagcaaca cgcagctcga gcgggaatac aaggccaagg gcgtgcgaaa
     2041 tccgctctcc ttctcggaca ccgtcggcaa gtatgtcgag gccgcgtggc gctaccgccg
     2101 ccagaacccc ctggccgcgg actttatcct gctgacgatg ctctggagca accgccgcgg
     2161 cgagtcggcc acgttcaagt ggcgcgacca agtgcccgac gcccaggcac cgaccgagcg
     2221 ctggatcgat gacaggctgg gggtggggca cgtgggcgac gccaagaacc gcggcgacca
     2281 cgagttcccc atcgggccgt tcgcgatgca gctcctgaag ctgcggcggg ccgagcagac
     2341 cgagggccaa gtctgggtgt tcccaggacg caatccgaag agcgagggcg gaaactacaa
     2401 ggatcccacc gtggcgatgg cgaccgtcaa ggagatggcc ggcgtcaagc aggcgcgcgg
     2461 ccacgacctg cggcgcacat tcggagcggc gtgcgagaag ctcgggttct cggaccgcca
     2521 gacaaagcgc atgctgggac acgccaccgg ctcgggacag tccgtaaacc gctacaccga
     2581 tccggaatgg acggacatag cagatcgcat gcgacgcgtc gaggagctga tcctgtccaa
     2641 ggcgccgaac gtctacaacg ccttgcggcc tgccggaacg cccgctttgc cggagcccga
     2701 ctttgagatg gcgcctgtcc cggagcgccc gacccggcgc gcgcctgagt gagggcagcc
     2761 gctaaggttc ttcgacgccc ttgagataga ggctgcaatt agcgaactgc tgcgtccaaa
     2821 gaccgttgct caggccgtct gcgaaagatg tcgatttcgc tgatcgcagt tccatgattc
     2881 gctgtcggga cgtttcgagc agtgcggcga ttgatttttt gatggtcgct tcactgactc
     2941 tccccaagaa ttcacgcggt atcgcatgcg ccttgagccg gccgtcgccg cgcgcgtccg
     3001 cgacggtcag cctcgagtcc tgtcgctcct gcgaagaaat atcgcagagc atgtcgagat
     3061 gcggcgtgtc aagcagggcg gtttcaaatc cgaactgcaa caccgcgtta ttgaactgag
     3121 ctcggagact tgtgagagtt tgccagggtg agtccgaaga cctcaagcgg cgtgtgccag
     3181 tcgtgagtgg cgcggggtcg cgtgttgagg ctgtc
//
LOCUS       DASVLN010000328         7185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_338865, whole genome shotgun sequence.
ACCESSION   DASVLN010000328 DASVLN010000000
VERSION     DASVLN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338865"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1522)
                     /gene="hemN"
                     /locus_tag="VF453_07960"
     CDS             complement(98..1522)
                     /gene="hemN"
                     /locus_tag="VF453_07960"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX7687625.1"
                     /translation="MTEAPVAAAAADRAALPSLALLHRIQGPGPRYTSYPTADRFGDG
                     FGAAELVAALDARRTESDAGTCAPLSLYVHVPFCESVCYYCACNKVITRHHERAAPYL
                     DALELEIALVTDQLGACRAVSQLHLGGGSPTFLSAAELARLMGAIRRSFFLTPEAELS
                     IEIDPRTVDAMRLQHLRALGFNRVSFGVQDFDPDVQRAVHREQPFEQVQALMAASREI
                     GFASINVDLIYGLPRQTPQTFARTLAQVAKLRPDRVALYAYAHLPERFKPQRRIHAAE
                     LPDGAQRVAMLAQAMDAFGAQGYDYIGMDHFALPGDALAVARRAGRLHRNFQGYSTQP
                     DCDLVGLGVSAIGRIGDCYYQNEKTLDAYYAALEADRLPVARGLTLDADDRVRRDIIM
                     ALMCQGRVDFADIRARHGVDMPAAFADELVRLAPLAAEGLVELRGDGIRVTPAGWFVV
                     RAIAMVFDRYLQGHAGRERFSRIV"
     gene            complement(1567..1806)
                     /locus_tag="VF453_07965"
     CDS             complement(1567..1806)
                     /locus_tag="VF453_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687626.1"
                     /translation="MRHRTLTILWPSFLMAGVLEGVVFSLVDPRDIGSIERWADGLSP
                     LGVYTLGFLLFWAVVSCASAITALLVVEQDGAPPP"
     gene            complement(1838..2101)
                     /locus_tag="VF453_07970"
     CDS             complement(1838..2101)
                     /locus_tag="VF453_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687627.1"
                     /translation="MPRPIAAVVPTVPWWRVPTGWLAFGLPLVAVVAASASAVIAARG
                     ADPVVDQRHAAEHQSADEQAEHGPQSDARLPAELARNHASMRK"
     gene            complement(2119..3576)
                     /gene="ccoG"
                     /locus_tag="VF453_07975"
     CDS             complement(2119..3576)
                     /gene="ccoG"
                     /locus_tag="VF453_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX7687628.1"
                     /translation="MTRDPGALAAARRPVIPIAEEPSVGLYRAERKIHPREIRGRFSQ
                     WRWIVVALTQLAFYGLPWIAWHGRQAVLFDLSARRFYLFGLVLYPQDFFYLAALLIVS
                     AYSLFLFTAVAGRLWCGFACPQTVYTELFLWIERRFEGDRSARMRLDAAPWTFDKLWR
                     KTGKQASWIALALWTGFTFVGFFTPIRTLASETAALSFGPWEWFWILFYGFATYGNAG
                     WMREQVCKYMCPYARFQSAMFDRDTMIVTYDGGRGEPRGPRAKTLAHADLRAAGKGEC
                     IDCTMCVQVCPTGIDIRKGLQYECIGCAACVDACDAVMDKVGYPRGLIRFATENGLEQ
                     GWSGAQMRRRVLRPRVLVYGGVLLVIVAAIGTSLALQRPYRVDVLRDRGTLAREVRGG
                     RIENVYRLQVMNASEAPQRFRVSVSGLDGIAVASEPTLDVEATGERALAVRVDIPAET
                     AERAGPGIRPIRFAIRREGDTREPLVERSTFLIPR"
     gene            complement(3593..4498)
                     /gene="ccoP"
                     /locus_tag="VF453_07980"
     CDS             complement(3593..4498)
                     /gene="ccoP"
                     /locus_tag="VF453_07980"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEX7687629.1"
                     /translation="MSDFFSPGWSIYVAVVTLASLAGCLALLVVASRARTNPKDDTGH
                     TWDGDLRELNNPMPRWWMVLFVITIVFGVGYLVLYPGLGSSKGLLGWSSAAQYRDEQA
                     RADAQLAGVYAKYASTPAPQLARDKGAMAIGERLFMNNCSGCHGSDARGGKGFPNLTD
                     DDWLYGGSPEAIVETITHGRQGMMPPMAAAVGDGYDVQNVAEYVLSLSNSPHDAAAAD
                     KGRAKFVVCAGCHGVDGKGNQAIGAPNLTDDTWLHGSGRAAIIRMVEHGKTNVMPAQE
                     RRLTPAQIHVVGAYVWGLSHAGTAN"
     gene            complement(4495..4644)
                     /locus_tag="VF453_07985"
     CDS             complement(4495..4644)
                     /locus_tag="VF453_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEX7687630.1"
                     /translation="MDLDLVRYAVTVLSFAVFAGIVAWALSPRNRAAFDEAQELPFLD
                     SGDPR"
     gene            complement(4656..5288)
                     /gene="ccoO"
                     /locus_tag="VF453_07990"
     CDS             complement(4656..5288)
                     /gene="ccoO"
                     /locus_tag="VF453_07990"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEX7687631.1"
                     /translation="MANDATTTKSGFSHAAIETNNFLMIVLILVVVAIGGLVEIVPLF
                     FQKSTTQPVEGLKPYTALQLAGRDVYLREGCYGCHSQMIRPLRAETLRYGHYSMAGES
                     VYDHPFQWGSKRTGPDLARVGGKYSDDWHRIHLNNPRDVVPESNMPAYSWLATGKVDP
                     AGMAPRMRALRRVGVPYTDADIAGAAAEVDGKTDLDALVAYLQVLGTAVK"
     gene            complement(5308..6753)
                     /gene="ccoN"
                     /locus_tag="VF453_07995"
     CDS             complement(5308..6753)
                     /gene="ccoN"
                     /locus_tag="VF453_07995"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEX7687632.1"
                     /translation="MVNTSATVPSDAVYDDQAVRLFSIAAVVWGVVGMLVGVYIAAEL
                     AFPDINFGIPWLSYGRLRPLHTNAVIFAFGGCALMATSYYVVQRTCQVRLLSSRLAAL
                     HFWGYQAVIVLAAITLPLGITRGKEYAELEWPIALLLAVVWVIYAVNFFGTIGIRKVR
                     HIYVANWFYGAFIIAVALLHVVNGASIPVSLTKSYSVYSGVQDAMVQWWYGHNAVGFF
                     LTAGFLGMMYYFIPKQAERPVYSYRLSIVHFWALIFTYMWAGPHHLHYTALPDWVQSV
                     GMLFSLVLLAPSWGGMINGIMTLSGAWHKLRDDPILKFLIVSLSFYGMSTFEGPMMSI
                     KTVNSLSHYTDWGIAHVHSGALGWVGLITMGSLYYLVPRMFGRTAMYSVRAIELHFWI
                     ATIGIVLYIASMWIAGVMEGVMWRTVNPDGTLAYSFVETVKATFPFYVVRLGGGLMYL
                     AGMLVMAWNVWKTATTGRYSPVRIPLAVANA"
     gene            complement(6794..6982)
                     /gene="ccoS"
                     /locus_tag="VF453_08000"
     CDS             complement(6794..6982)
                     /gene="ccoS"
                     /locus_tag="VF453_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX7687633.1"
                     /translation="MESLYLLVPLSALLVLALLGLFAWALDAGQFDDLEREGERILGD
                     AGLALDGDQAPRGDAPEE"
     gene            complement(6987..>7185)
                     /locus_tag="VF453_08005"
     CDS             complement(6987..>7185)
                     /locus_tag="VF453_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687634.1"
                     /translation="ADVARARTLARRTLRVVHQNLAWAAAYNAACVPLAVTGHLPPWA
                     AGLGMACSSLVVVLNSTRLGR"
BASE COUNT         1122 a         2445 c         2628 g          990 t
ORIGIN      
        1 cgcgtgaccg cggcgcaggg tgcgccgcac atcagcgcgc aatgcggcgc cgcggcggcg
       61 cccagcgcga gtccggccgc gacgagcgcg acgttcatca gacgatgcgc gagaagcgct
      121 cgcgtcccgc atgcccctgg aggtatctgt cgaagaccat cgcgatcgcc cgcacgacga
      181 accagccggc cggcgtgacg cggatgccgt cgccgcgcag ctccacgagc ccttcggccg
      241 ccagcggcgc caatcgcacg agctcgtccg cgaacgcggc gggcatgtcg acgccgtggc
      301 gcgcgcggat gtccgcgaag tcgacgcgtc cctggcacat gagcgccatg atgatgtcgc
      361 gccgcacgcg gtcgtcggcg tccagcgtca gtccgcgggc gaccggcagc cggtcggcct
      421 ccagggcggc gtagtacgcg tccagcgtct tctcgttctg gtagtagcag tcgccgatgc
      481 ggccgatcgc ggagacgccc agccccacca ggtcgcagtc gggttgcgtg ctgtagccct
      541 ggaagttgcg atgcagccga cccgcgcgac gggcgaccgc gagcgcgtcg cccggcagcg
      601 cgaagtggtc catgccgatg tagtcgtatc cctgggcgcc gaacgcgtcc atcgcctggg
      661 cgagcatcgc gacgcgctgc gccccgtcgg gcagctcggc ggcgtggatg cggcgctgcg
      721 gcttgaagcg ctcgggcagg tgggcatagg cgtagagcgc gacgcggtcg ggccgcagct
      781 tcgcgacctg cgccagcgtg cgcgcgaagg tctgcggcgt ctggcgcggc aggccgtaga
      841 tcaggtcgac gttgatcgac gcgaagccga tctcgcgcga cgccgccatc agcgcctgca
      901 cctgctcgaa gggctgctcg cggtgcacgg cgcgctgcac atcgggatcg aagtcctgca
      961 cgccgaagct cacgcggttg aagccgagcg cgcgcaggtg ctgcaggcgc atcgcatcga
     1021 cggtgcgcgg gtcgatctcg atcgacagct cggcctcggg cgtcaggaag aacgaccggc
     1081 ggatcgcgcc catcaggcgc gcgagctccg cggccgacag gaaggtcggc gagccgccgc
     1141 ccaggtgcag ctgcgacacc gcgcggcacg cgcccagctg gtcggtgacg agcgcgatct
     1201 cgagctccag ggcgtcgagg tacggggccg cgcgctcgtg atggcgcgtg atgaccttgt
     1261 tgcacgcgca gtagtagcac accgactcgc agaacgggac gtgcacgtag agcgacagcg
     1321 gcgcgcaggt gccggcgtcc gattcggtgc gacgggcgtc cagcgcggcc acgagctccg
     1381 cggcgccgaa gccgtcgccg aagcggtcgg ccgtgggata ggaggtatag cgcgggccgg
     1441 ggccctggat gcgatgcagc agggccaggg agggcagggc ggcgcggtcg gcggctgcgg
     1501 cggcgacggg agcttcggtc atgccggcat ggtgcgcgcc gtgccgcgcc gggtgcttgc
     1561 gctggctcaa ggcggcgggg cgccgtcctg ttccacgacg agcagggcgg tgatcgcgct
     1621 cgcgcacgac acgacggccc agaagagcag gaagccgagc gtgtagacgc ccagcggcga
     1681 gagcccgtcg gcccagcgct cgatgctgcc gatgtcgcgc ggatcgacga gcgagaacac
     1741 gacgccctcg agcacgcccg ccatgaggaa ggacggccag aggatcgtca gcgtgcggtg
     1801 ccgcatgggc tcgcctcccg cggtcggcgc gcggccgtca cttgcgcatc gaggcatggt
     1861 tgcgcgccag ctcggccggc agccgggcgt ccgactgcgg gccgtgctcg gcctgctcgt
     1921 cggccgactg gtgctcggcg gcgtggcgct ggtcgacgac gggatcggcg ccgcgcgccg
     1981 cgatcaccgc cgacgccgac gccgcgacca cggccaccag cggcaagccg aacgcgagcc
     2041 agccggtcgg cacgcgccac caggggacgg tcgggacgac ggcggcgatg ggacggggca
     2101 tggcggtctc ctctcgtttc agcgggggat caggaaggtc gagcgctcga ccagcggctc
     2161 gcgggtgtcg ccttcgcgac ggatcgcgaa gcggatgggg cggatgccgg ggccggcgcg
     2221 ttccgcggtc tcggccggga tgtccacgcg caccgcgagc gcgcgctcgc cggtggcctc
     2281 gacgtccagc gtcggctcgg aggcgacggc gatgccgtcc aggccggaga cgctgacgcg
     2341 gaagcgctgc ggcgcctcgc tcgcgttcat gacctgcagg cggtagacgt tctcgatgcg
     2401 tccgccgcgc acctcgcgcg cgagcgtccc gcggtcgcgc agcacgtcga cgcgataggg
     2461 ccgctgcagc gcgaggctcg tgccgatcgc ggcgacgatg accagcagca ccccgccgta
     2521 gaccagcacg cgcggccgca gaacgcggcg ccgcatctgc gcgccgctcc agccctgctc
     2581 caggccgttc tcggtggcga agcggatcag gccgcgcggg tagccgacct tgtccatcac
     2641 cgcgtcgcag gcgtcgacgc aggcggcgca gccgatgcac tcgtactgca ggcccttgcg
     2701 gatgtcgatg ccggtcgggc acacctgcac gcacatcgtg cagtcgatgc actcgccctt
     2761 gccggcggcg cgcaggtcgg cgtgcgccag cgtcttcgcg cgcgggccgc gcggctcgcc
     2821 gcggccgccg tcgtaggtga cgatcatcgt gtcgcggtcg aacatcgcgc tctggaagcg
     2881 cgcatacggg cacatgtact tgcagacctg ctcgcgcatc cagccggcgt tgccgtaggt
     2941 ggcgaagccg tagaacagga tccagaacca ctcccacggg ccgaacgaca gcgccgccgt
     3001 ctcgctggcg agggtgcgga tcggcgtgaa gaagccgacg aaggtgaagc ccgtccacag
     3061 cgccagcgcg atccagctcg cctgcttgcc cgtcttgcgc cagagcttgt cgaaggtcca
     3121 cggcgcggcg tcgagccgca tgcgcgcgct gcggtcgccc tcgaagcggc gctcgatcca
     3181 gaggaacagc tcggtgtaga ccgtctgcgg gcaagcgaac ccgcaccaca ggcggccggc
     3241 caccgccgtg aagaggaaca ggctgtacgc gctgacgatc agcagcgccg cgaggtagaa
     3301 gaagtcctgc ggatacagca ccagcccgaa caggtagaag cgccgcgcgg agaggtcgaa
     3361 cagcaccgcc tgccggccgt gccaggcaat ccagggcagg ccgtagaacg cgagctgcgt
     3421 cagcgcgacg acgatccagc gccactgcga gaagcggccg cggatctcgc gcggatggat
     3481 cttcctttcg gcgcggtaca ggccgaccga gggctcctcc gcgatcggga tcacggggcg
     3541 gcgcgcggcg gccagtgcgc cggggtcgcg ggtcatgcgt gcgtcctcgt cctcagttgg
     3601 ccgtgccggc gtgcgacagg ccccagacgt aggcgccgac cacgtggatc tgcgcgggcg
     3661 tcagccggcg ttcctgcgcc ggcatcacgt tggtcttgcc gtgttcgacc atgcggatga
     3721 tcgcggcgcg gccggaaccg tgcagccacg tgtcgtccgt caggttgggc gcgccgatcg
     3781 cctggttgcc cttgccgtcg acgccgtggc agcccgcgca gacgacgaac ttggcgcggc
     3841 ccttgtccgc agcggcggcg tcgtgcgggc tgttggacag gctcagcacg tactccgcga
     3901 cgttctgcac gtcgtagccg tcgcccaccg cggcggccat cggcggcatc atcccctggc
     3961 ggccgtgggt gatggtctcg acgatggcct cggggctgcc gccgtagagc cagtcgtcgt
     4021 cggtcaggtt ggggaagccc ttgccgccgc gcgcgtccga gccgtggcag ccggagcagt
     4081 tgttcatgaa cagccgctcg ccgatcgcca tcgcgccctt gtcgcgcgcg agctgcggcg
     4141 ccggcgtcga ggcgtacttc gcatagacgc cggcgagctg cgcgtccgcc ctggcctgtt
     4201 cgtcgcggta ctgcgccgcg ctgctccagc ccagcaggcc cttggagctg ccgagtcccg
     4261 gatacagcac gaggtagccg acgccgaaga cgatggtgat gacgaacagc accatccacc
     4321 agcgcggcat cgggttgttc agctcgcgca ggtcgccgtc ccaggtgtgg ccggtgtcgt
     4381 ccttgggatt ggtgcgggca cggctggcga cgaccagcag cgcgaggcag ccggccaggc
     4441 tggccagcgt gacgacggcg acgtagatcg accagccggg ggagaagaag tcgctcatcg
     4501 cgggtctcct gaatcgagga agggcagctc ctgcgcctcg tcgaaggcgg cgcggttgcg
     4561 cggcgacagc gcccacgcga cgatgccggc gaacacggcg aaggacagca cggtcacggc
     4621 gtagcgcacg aggtcgagat ccatggctcg gctcctcact tcacggcggt gccgagcacc
     4681 tgcaggtagg cgacgagcgc gtcgaggtcg gtcttgccgt cgacctccgc cgcggcgccg
     4741 gcgatgtcgg cgtcggtgta gggcacgccg acgcggcgca gggcgcgcat gcgcggcgcc
     4801 atgccggccg gatcgacctt gccggtggcc agccacgagt aggcgggcat gttggactcg
     4861 ggcaccacgt cgcgcgggtt gttcaggtgg atgcggtgcc agtcgtcgct gtacttgccg
     4921 cccacgcgcg cgaggtcggg cccggtgcgc ttgctgcccc actggaacgg atggtcgtag
     4981 accgactcgc cggccatcga gtagtggccg tagcgcagcg tctcggcgcg cagcgggcgg
     5041 atcatctgcg agtggcagcc gtagcaaccc tcgcgcaggt agacgtcgcg cccggccagc
     5101 tgcagcgcgg tgtagggctt gagcccctcg accggctggg tcgtcgactt ctggaagaac
     5161 agcgggacga tctcgaccag gccgccgatc gcgacgacca ccaggatcag cacgatcatc
     5221 aggaagttgt tcgtctcgat cgcggcgtgc gagaacccgg acttggtggt cgtggcgtcg
     5281 ttggccatgt tgatggttcc tttgcgttca ggcgttcgcc acggccagcg ggatgcgcac
     5341 ggggctgtag cgcccggtcg tggcggtctt ccagacgttc caggccatca ccagcatgcc
     5401 ggccaggtac atcaggccgc cgcccagccg cacgacgtag aaggggaagg tcgccttgac
     5461 cgtctcgacg aagctgtagg cgagcgtgcc gtcgggattg acggtgcgcc acatgacgcc
     5521 ttccatcacg cccgcgatcc acatggacgc gatgtacagc acgatgccga tggtcgcgat
     5581 ccagaagtgc agctcgatcg cccgcacgct gtacatcgcg gtgcggccga acatgcgcgg
     5641 caccaggtag tacagcgacc ccatcgtgat caggccgacc cagccgagcg cgcccgagtg
     5701 cacgtgcgcg ataccccagt ccgtgtagtg cgagagcgag ttgaccgtct tgatcgacat
     5761 catcgggccc tcgaaggtcg acatgccgta gaacgacagg ctgacgatca ggaacttcag
     5821 gatcgggtcg tcgcgcagct tgtgccaggc gcccgacagg gtcatgattc cgttgatcat
     5881 cccgccccag ctcggcgcga gcagcacgag ggagaacagc atgcccacgc tctgcaccca
     5941 gtcgggcagc gccgtgtagt gcaggtggtg cggacccgcc cacatgtagg tgaagatcag
     6001 cgcccagaag tggacgatgg acaggcggta gctgtagacg gggcgctcgg cctgcttggg
     6061 gatgaagtag tacatcatcc cgaggaagcc cgcggtcagg aagaagccga ccgcgttgtg
     6121 gccgtaccac cactgcacca tcgcatcctg aaccccggaa tacaccgagt agctcttggt
     6181 caggctcacc gggatgctgg cgccgttgac cacgtgcagc agcgccaccg cgatgatgaa
     6241 ggcgccgtag aaccagttgg cgacgtagat gtggcgcacc ttgcggatgc cgatggtgcc
     6301 gaagaagttg accgcgtaga tcacccagac gaccgccagc agcagcgcga tgggccattc
     6361 gagctcggcg tattccttgc cgcgcgtgat gcccagcggc agcgtgatcg cggccagcac
     6421 gatcaccgcc tggtagcccc agaagtgcag cgccgcgagc cgggacgaca gcagccgcac
     6481 ctggcaggtt cgctgcacga cgtagtagct ggtggccatc agggcgcagc cgccgaaggc
     6541 gaagatcacg gcattggtgt gcagcgggcg caggcggccg tagctgagcc acgggatgcc
     6601 gaagttgatg tcggggaagg ccagctccgc ggcgatgtag acgccgacca gcatgcccac
     6661 cacgccccag acgaccgccg cgatagagaa caggcgcacg gcctggtcgt cgtagacggc
     6721 gtcgctcggc acggtcgcgg aagtgttgac catcgaatcc tccaatcgat ccggcacgtg
     6781 ccggattgcc gcgtcattct tccggggcgt cgcctcgagg tgcttgatcg ccgtcaagcg
     6841 ccaggccggc gtcgccgagg atgcgctcgc cctcgcgctc gaggtcgtcg aactggccgg
     6901 cgtcgagcgc ccacgcgaac aggcccagca gcgcgagcac gagcagcgcc gacagcggca
     6961 cgagcaggta caggctctcc atcgcgtcag cgccccagcc gcgtcgagtt cagcacgacg
     7021 accagcgagc tgcacgccat gcccagcccc gcggcccagg gcggcaggtg gccggtcacc
     7081 gccagcggca cgcaggccgc gttgtaggcc gcggcccagg cgaggttctg gtgcaccacg
     7141 cgcagcgtgc gtcgcgccag cgtgcgggcg cgcgcgacgt cggcg
//
LOCUS       DASVLN010000329         5004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_501813, whole genome shotgun sequence.
ACCESSION   DASVLN010000329 DASVLN010000000
VERSION     DASVLN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501813"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VF453_08010"
     CDS             complement(<1..850)
                     /locus_tag="VF453_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7687635.1"
                     /translation="MAELRLRGVRKAFGSTDVLRGIDLDIADGEFMVFVGPSGCGKST
                     LLRLIAGLDDADGGEILIGGDAMDGVSPAERGVAMVFQSYALYPHMTVAQNLGFALRM
                     AGRSKRDIAAAVDRAAEILQLGPLLGRLPKALSGGQRQRVAIGRAIVREPRVFLFDEP
                     LSNLDAALRVQMRLELARLHRELGTTMVYVTHDQVEAMTLGDRIALFNGGSLEQAGPP
                     LELYERPRTRFVAGFLGQPQMNFLPAARLDAAERRLFAGDAGALAADWTLGVRPEHLL
                     LRGADGP"
     gene            complement(843..2210)
                     /locus_tag="VF453_08015"
     CDS             complement(843..2210)
                     /locus_tag="VF453_08015"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX7687636.1"
                     /translation="MSNLQHPFALSPETRTTLSSDFLWGVATSAYQIEGGAAAGGRGP
                     SIWDTFSHTPGKTVRGETGDIACDHYHRWPEDVALMAGLGVDAYRFSISWSRVQPLGH
                     GAWNEEGLAFYERLVDALLARGIAPHSTLYHWDLPQGLQDRGGWAERETALRFADYAE
                     KIGRRLGDRLAGIATHNEPWCTATLGHERGQFAPGFKDAALAADVSHHLLLSHGLALQ
                     AMRAAGTRAPLGIVLNQSSTSAATDSAADRALAATEYAKFVRWYMEPLFEGRYPPDTG
                     IAHYPRNIRPGDFAVIRQPLDFLGINYYTRIWASSAQPPVPAPKALGVTDMGWEIHPE
                     GLTELLVGLRERYAGLPPIYLMENGMANAGDVVVEGRVADAPRIDYMRRHLEALARAR
                     EAGVDVRGFFYWSLMDNYEWDSGYGKRFGLVHVDYATLRRTPKDSALWYRETIAAVRA
                     EAAHG"
     gene            complement(2299..3213)
                     /locus_tag="VF453_08020"
     CDS             complement(2299..3213)
                     /locus_tag="VF453_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7687637.1"
                     /translation="MTTAHPMPATSMPGSARRGSAPARSRARSRNRRGALVAGRLVVL
                     AGAAVMLLPFWFMFVFATHSNGEILSMPPPMWFGHSLGDNLRDLLERLPTFWQNLGWS
                     FAVAIATTLLNLFFCSLAGHAFALLDFAGKERLYAAVMLTMLLPGFLFMVPTALTMSW
                     LGWTNEERALVVPGACSALGIFLMRQYIEAAIPRDLVEAARLDGCSEFGLWWRVVLPL
                     LGPALGTLALITFIGSWNNFMGPLVVLRDMDRYTLPLALRSLQGSGQTPWGAMCAGAA
                     IAVLPLLAMFALASRRLIDGLTAGAVKH"
     gene            complement(3210..4151)
                     /locus_tag="VF453_08025"
     CDS             complement(3210..4151)
                     /locus_tag="VF453_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7687638.1"
                     /translation="MPSVTSAPRRRPLIPARWAPYVFISPFFVLFAAFGLFPLLFSIA
                     LSFHQWDPVGGVWNMRWVGLDNFAYAVTRDDWLRKSMFNTVWIALVSGLPQHLVALPL
                     AWFLHRAFGRWRNAVVGAYFLPFITSTVAISLVFSSLFSKDFGLVNAAFATLREVPVL
                     GWLMPGQSIDWSQPGYTKWMISAVVFWRYVGWNTVLYLSAIQTIPTDLLEAAEIDGAD
                     ARQRFVHIVLPLLKPMVFFAVTLSLIGNLQLFEEPFILTGGTGGIDQAGKTAAMHMYA
                     VAFMDGDFGTASAIAWLLFLLIAGATALNKRLLGARA"
     gene            complement(4159..>5004)
                     /locus_tag="VF453_08030"
     CDS             complement(4159..>5004)
                     /locus_tag="VF453_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7687639.1"
                     /translation="GDIGPGALFYRADLLARAGVTEQDLTRDWDSYIAAGVKLKRLTG
                     VYLLASAIDLKDIVIRAGLADGQGVYFDGAGRPQVESPRFVRAFTIARAARRAGIDAR
                     IAAWSSEWSEGFRRDRVATQMMGSWLAGHLKNWIVPAQSGLWRSAPLPGGTYASWGGS
                     YWAIPVRAEHKALAWDFIRTLSLDRAQQLEAFRRLDAFPALLAAQNDDYLAQPIAYLG
                     GQAARLQWRAASERIPALHVDRYDQVAKDIVNAELDQVLEHDKPIPQALSDARLAIER
                     RVRRY"
BASE COUNT          796 a         1713 c         1845 g          650 t
ORIGIN      
        1 ccgggccgtc ggcgccgcgc agcagcaggt gctccggccg cacgcccagc gtccagtccg
       61 cggcgagcgc accggcgtcg cctgcgaaca gccggcgctc ggcagcgtcg agccgcgccg
      121 ccggcaggaa gttcatctgc ggctgtccga ggaagccggc gacgaagcgc gtgcgcggac
      181 gctcgtagag ctccagcggc ggccccgcct gctcgaggct gccgccgttg aacagcgcga
      241 tgcggtcgcc cagcgtcatc gcctcgacct ggtcgtgcgt gacatagacc atcgtcgtgc
      301 cgagttcccg atgcaggcgc gcgagctcca ggcgcatctg cacgcgcagc gcggcgtcga
      361 ggttcgacag cggctcgtcg aacaggaaca cgcgcggctc ccgcacgatc gcgcggccga
      421 tggccacgcg ctggcgctgc ccgccggaca gcgccttggg caaccgtccg agcagcggcc
      481 ccagctgcag gatctccgcc gcccgatcca ccgccgcggc gatgtcgcgc ttcgagcgcc
      541 ccgccatgcg cagcgcgaac ccgaggttct gcgccaccgt catgtgcgga tacagcgcgt
      601 agctctggaa caccatcgcc accccgcgtt cggccggcga gacgccgtcc atcgcgtcgc
      661 cgccgatgag gatctcgccg ccgtcggcgt cgtcgagccc cgcgatcagc cgcagcagcg
      721 tggacttgcc gcagcccgac ggtccgacga agaccatgaa ctcgccgtcc gcgatgtcga
      781 ggtcgatgcc gcgcagcacg tcggtcgagc cgaaggcctt gcgcacgccg cgcagccgca
      841 gctcagccat gggcggcctc cgcgcgcacc gccgcgatcg tctcgcggta ccacagcgcg
      901 ctgtccttgg gcgtgcgccg cagggtcgcg tagtccacgt gcacgagccc gaagcgcttg
      961 ccgtagcccg agtcccactc gtagttgtcc atcaggctcc agtagaagaa gccgcggacg
     1021 tcgacgccgg cctcgcgggc gcgcgcgagc gcctcgaggt ggcggcgcat gtagtcgatg
     1081 cgcggcgcgt cggcgacgcg gccctcgacg acgacgtcgc ccgcgttggc catcccgttt
     1141 tccatcaggt agatcggcgg caggccggca tagcgctcgc gaaggccgac gagcagctcc
     1201 gtcaggccct cgggatggat ctcccagccc atgtcggtga cgcccagcgc cttgggcgca
     1261 ggcaccggcg gctgcgcgct gctggcccag atgcgcgtgt agtagttgat gccgaggaag
     1321 tccagcggct gacggatcac cgcgaagtcc ccgggccgga tgttgcgggg gtagtgcgcg
     1381 atcccggtgt ccggcggata acggccctcg aagagcggct ccatgtacca gcggacgaac
     1441 ttcgcgtact ccgtggcggc gagcgcccgg tcggcggcgc tgtcggtggc cgcgctggtg
     1501 gacgactggt tcagcacgat gcccagcggg gcgcgcgtcc ccgcggcgcg catcgcctgc
     1561 agcgccagcc cgtgcgacag gagcaggtga tgcgacacgt cggccgcgag cgccgcgtcc
     1621 ttgaagccgg gcgcgaactg cccgcgctcg tggcccagcg tcgcggtgca ccagggctcg
     1681 ttgtgcgtgg cgatgccggc gagccggtcg cccaggcggc ggccgatctt ctccgcgtag
     1741 tcggcgaagc gaagcgccgt ctcgcgctcc gcccagccgc cgcgatcctg caggccttgc
     1801 gggaggtccc agtgatagag cgtcgaatgc ggggcgatgc cgcgggcgag cagcgcgtcg
     1861 accaggcgct cgtagaaggc caggccttcc tcgttccacg cgccgtggcc cagcggctgc
     1921 acgcgcgacc acgagatcga gaagcgatag gcgtcgacgc ccaggccggc catcagcgcg
     1981 acgtcctccg gccagcggtg gtagtggtcg caggcgatgt cgccggtctc gccgcgcacc
     2041 gtcttgccgg gcgtgtgcga gaaggtgtcc cagatcgacg ggccgcggcc gccggcggcg
     2101 gcgccgcctt cgatctggta cgcgctggtg gcgacgcccc agaggaagtc gctcgagagc
     2161 gtcgtgcgcg tctcgggcga gagcgcgaag ggatgttgca gattcgacat cgatcggggt
     2221 ctttgcaggt ttgaaatcgt tatcagagca gagctcgaag aagccgcggg ccgcggcttg
     2281 gcgtgggggg cggcgggatc agtgcttcac ggcacccgcg gtgaggccat cgatcagccg
     2341 gcgcgaggcg agcgcgaaca tcgccagcag cggcaggacg gcgatggcgg cgcccgcgca
     2401 catcgcgccc cagggcgtct ggccggaccc ctgcagcgag cgcagcgcca ggggcagcgt
     2461 gtagcgatcc atgtcgcgca gcaccaccag cggccccatg aagttgttcc aggagccgat
     2521 gaacgtgatc aacgcgagcg tcccgagcgc cggcccgagc agcgggagga cgacgcgcca
     2581 ccagagcccg aactcgctgc agccgtccag gcgcgcggcc tccacgaggt cgcgcgggat
     2641 cgcggcctcg atgtactggc gcatcaggaa gatgcccagc gcgctgcagg cgccggggac
     2701 gaccagcgcg cgctcctcgt tggtccagcc gagccagctc atggtcaagg ccgtgggcac
     2761 catgaacagg aagcccggca gcagcatcgt cagcatcacc gcggcataca ggcgctcctt
     2821 gcctgcgaag tccagcagtg cgaacgcgtg gccggcgagg ctgcagaaga acaggttcag
     2881 cagagtcgtc gcgatggcga cggcgaagct ccagccgagg ttctgccaga aggtcggcag
     2941 gcgctcgagc agatcgcgca ggttgtcgcc gagcgagtgg ccgaaccaca ttggcggcgg
     3001 catcgacagg atctcgccgt tgctgtgcgt ggcgaagacg aacatgaacc agaacggcag
     3061 cagcatcacc gccgcgccgg cgagcacgac gaggcggccc gcgacgagcg cgccgcgacg
     3121 gttgcgcgaa cgcgcgcgcg agcgggcagg tgcgctgccg cgtcgcgcgg agccgggcat
     3181 cgaggtggcg ggcatggggt gggccgtggt catgcgcgag ctccgagcag gcgcttgttg
     3241 agggcggtcg cgccggcgat gagcaggaac agcagccagg cgatggccga ggcggtaccg
     3301 aagtcgccgt ccatgaaggc gacggcgtac atgtgcatcg ccgccgtctt gcccgcctgg
     3361 tcgatgccgc cggtgccgcc ggtcaggatg aagggctcct cgaacagttg caggttgccg
     3421 atgagcgaca gcgtcaccgc gaagaacacc atgggcttga gcagcggcag gacgatgtgc
     3481 acgaagcgct ggcgcgcgtc ggcgccgtcg atctcggcgg cctcgagcag gtcggtcggg
     3541 atcgtctgga tcgccgacag gtacagcacc gtgttccatc cgacgtagcg ccagaagacc
     3601 accgccgaga tcatccactt cgtgtagccg ggctggctcc agtcgatcga ctgcccgggc
     3661 atcagccagc cgagcacggg cacctcgcgc agcgtggcga aggcggcgtt cacgaggccg
     3721 aagtccttgg agaacagcga cgagaagacc agcgagatgg ccaccgtcga cgtgatgaac
     3781 ggcaggaagt acgcgccgac caccgcgttg cgccagcgcc cgaacgcgcg gtgcaggaac
     3841 cacgccagcg gcagcgcgac gagatgctgc ggcaggccgg agaccagcgc gatccagacg
     3901 gtgttgaaca tcgacttgcg cagccagtcg tcgcgcgtga cggcgtacgc gaagttgtcg
     3961 aggccgaccc agcgcatgtt ccatacgccg cccaccggat cccattggtg gaacgacagg
     4021 gcgatggaga acagcagcgg gaacaggccg aaggccgcga acaggacgaa gaaggggctg
     4081 atgaagacgt agggcgccca gcgcgccggg atcagcgggc ggcggcgcgg cgcggaagtg
     4141 acggacggca tggcggtctc aatagcggcg cacgcggcgc tcgatggcga ggcgggcgtc
     4201 ggagagcgcc tgcgggatgg gcttgtcgtg ctcgagcacc tggtcgagct cggcgttcac
     4261 gatgtccttc gccacctggt cgtagcggtc gacgtgcagc gcggggatgc gttcgctcgc
     4321 ggcgcgccac tgcaggcgcg ccgcctggcc gcccaggtag gcgatgggtt gcgcgaggta
     4381 gtcgtcgttc tgcgcggcca gcagcgccgg aaacgcgtcg agccgccgga aggcctcgag
     4441 ctgctgcgcg cgatccaggc tcaaggtgcg gatgaagtcc cacgcgagcg ccttgtgctc
     4501 ggcacgcacc gggatcgccc agtacgagcc gccccaggac gcgtacgtgc cgcccggcag
     4561 cggcgccgag cgccacaggc cggactgcgc gggcacgatc cagttcttca ggtggcccgc
     4621 gagccaagag cccatcatct gcgtggccac gcggtcgcgc cggaagcctt cgctccattc
     4681 gctgctccat gccgcgatgc gcgcgtcgat gccagcgcgg cgcgcggcgc gggcgatcgt
     4741 gaaggcccgt acgaagcgcg gcgactccac ctgcgggcgc cccgcgccgt cgaaatacac
     4801 gccctggccg tccgccaggc cggcgcggat cacgatgtcc ttcaggtcga tcgcgctggc
     4861 gagcaggtag acgccggtca ggcgcttgag cttgacgccg gcggcgatgt aggagtccca
     4921 gtcgcgcgtg agatcctgct cggtgacacc cgcgcgcgcg aggaggtcgg cgcggtagaa
     4981 cagcgcgccg gggccgatgt cgcc
//
LOCUS       DASVLN010000330         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_178563, whole genome shotgun sequence.
ACCESSION   DASVLN010000330 DASVLN010000000
VERSION     DASVLN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178563"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VF453_08035"
     CDS             <1..1180
                     /locus_tag="VF453_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7687640.1"
                     /translation="EAKAAAVDQATLRVSIEKVDQLINLVGELVITQAMLAQNSREGQ
                     LNQQLLAGLTDLERNTRDLQEAVMSIRMIPMSMVFNRFPRMLRDLAGKLGKKVELVTQ
                     GEATELDKSLVEKITDPLTHLVRNSCDHGIESPADRLAKGKPEHGTVTLVASHQGGSI
                     VIEVRDDGRGLSRPKLLAKARERGIDAPDSLTDQEVYALIFAPGFSTAEQVTDVSGRG
                     VGMDVVKKNITALGGTVEIDSAEGYGMSVKVRLPLTLAIMDGMSIGVGDECYILPLAS
                     VVESFQVAPGMIKTIGGSGRVVEVRDEFMPVIDLERVFDVPRFDFEHVSNIMVVIEAE
                     GARVALLVDELLGQQQVVVKNLEANYRKVQDVSGATIMGDGRVALILDTGSLVRRTRH
                     "
     gene            1282..>2489
                     /locus_tag="VF453_08040"
     CDS             1282..>2489
                     /locus_tag="VF453_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7687641.1"
                     /translation="MLTNLSTPVRAAILYSLLFGSQGVVIAGLSFGWSPAAIITSGLV
                     LVSLAGILFFLSVQMVKTVVGEFTRHAKLMSQGDLSHEIPILGGGESVGALRALKAMQ
                     EELRKTIGAIRSGSHEVSTASTEIATGNQDLSQRTEQTASNLQQTASSLSQLTGNVRQ
                     SADAAAQANQLASSATQVARRGGEVVSQVVSTMEEINSSSKKIADIIGVIDGIAFQTN
                     ILALNAAVEAARAGEQGRGFAVVAGEVRSLAQRSAEAAKEIKSLIGTSVDKVETGSKL
                     VQDAGSTMSEIVASVQRVTDIIGEISAASLEQSQGIGQVNEAVNRLDQMTQQNAALVE
                     QSAAAAESLKEQAHRMTTVVAGFRTASDFAIAPATKPAPVAHKPAASKPAASAVAAAR
                     PAAPKPAAAKP"
BASE COUNT          425 a          904 c          828 g          332 t
ORIGIN      
        1 cgaggccaag gccgccgcgg tcgaccaggc gacgctgcgc gtgtcgatcg agaaggtcga
       61 ccagctgatc aacctcgtcg gcgaactcgt catcacgcag gccatgctgg cccagaacag
      121 ccgcgagggc cagctgaacc agcaactgct cgccggcctg acggacctgg agcgcaacac
      181 ccgcgacctg caggaagcgg tgatgtcgat ccggatgatc ccgatgtcga tggtcttcaa
      241 ccgcttcccg cgcatgctgc gcgacctcgc cggcaagctg ggcaagaagg tcgaactggt
      301 cacgcagggc gaagccaccg agctggacaa gagcctggtc gagaagatca ccgacccgct
      361 cacccacctc gtgcgcaact cctgcgacca cggcatcgag tcgcctgccg accgcctggc
      421 caagggcaag cccgagcacg gcacggtcac gctggtcgcg tcgcaccagg gcggctccat
      481 cgtgatcgag gtgcgcgacg acggccgcgg cctgtcgcgc cccaagctgc tggccaaggc
      541 ccgcgagcgc ggcatcgacg ccccggactc gctgaccgac caggaggtct acgcgctgat
      601 cttcgcgccg ggcttctcga cggcggagca ggtgacggac gtctcgggcc gcggcgtcgg
      661 catggacgtg gtcaagaaga acatcacggc gctgggcggc acggtggaga tcgactccgc
      721 cgagggctac ggcatgagcg tcaaggtgcg cctgccgctg accctggcca tcatggacgg
      781 catgtccatc ggcgtcggcg acgagtgcta catcctgccg ctcgcctcgg tggtggagtc
      841 cttccaggtc gcccccggca tgatcaagac gatcggcggc tccggccgcg tggtcgaggt
      901 gcgcgacgag ttcatgcccg tcatcgacct cgagcgcgtg ttcgacgtgc cccgcttcga
      961 cttcgagcac gtcagcaaca tcatggtcgt gatcgaggcc gagggcgcgc gcgtcgcgct
     1021 gctggtcgac gaactgctcg gacagcagca ggtcgtcgtc aagaacctcg aggcgaacta
     1081 ccgcaaggtg caggacgtct cgggcgccac gatcatgggc gacggccgcg tcgcgctgat
     1141 cctggacacc ggatcgctgg tccgccgcac ccgccattga ctgccgaagg cggcgcgagc
     1201 cgcccgccag gccaacgccg cgcggcgccc cgcgcccgcg gcaccccatg aaacgcatca
     1261 cgaactgaaa cggcccactc catgttgacc aatctttcca cgcccgtgcg cgccgccatc
     1321 ctctactcgc tgctgttcgg cagccagggg gtcgtcatcg ccggcctgag cttcggctgg
     1381 tcgcccgcgg cgatcatcac gtccggcctc gtcctcgtca gcctcgcagg catcctgttc
     1441 ttcctgtccg tgcagatggt caagacggtg gtcggcgagt tcacgcgcca tgccaagctg
     1501 atgtcgcagg gcgacctgtc gcacgagatc cccatcctgg gcggcggcga gtccgtcggc
     1561 gccctgcgtg cgctcaaggc gatgcaggag gagctgcgca agacgatcgg cgcgatccgc
     1621 tccggctcgc acgaggtctc cacggcctcg accgagatcg ccaccggcaa ccaggatctc
     1681 agccagcgca ccgagcagac cgccagcaac ctgcagcaga ccgccagctc gctgtcgcag
     1741 ctgaccggca acgtccgcca gagcgccgac gcggccgccc aggccaacca gctcgcgagc
     1801 tcggcgaccc aggtcgcgcg ccgcggcggc gaggtcgtct cgcaggtcgt ctcgacgatg
     1861 gaggagatca actcctcgtc caagaagatc gccgacatca tcggcgtgat cgacggcatc
     1921 gcgttccaga ccaacatcct ggccctgaac gccgccgtgg aagcggcccg cgcgggcgag
     1981 cagggccgcg gcttcgcggt cgtcgccggc gaggtgcgtt cgctggccca gcgctcggcc
     2041 gaagcggcca aggagatcaa gtcgctgatc ggcacgagcg tcgacaaggt cgagaccggc
     2101 tcgaagctgg tgcaggacgc cggctccacg atgagcgaga tcgtcgccag cgtgcagcgc
     2161 gtgaccgaca tcatcggcga gatcagcgcc gcgtcgctcg agcagtcgca gggcatcggc
     2221 caggtcaacg aggccgtcaa ccgcctcgac cagatgacgc agcagaacgc cgcgctggtg
     2281 gagcaatccg ccgccgccgc cgagagcctg aaggaacagg cgcatcgcat gaccacggtc
     2341 gtcgccggct tccgcacggc gtcggacttc gcgatcgccc cggccaccaa gccggcgccg
     2401 gtcgcccaca agccggcggc cagcaagccg gccgcctcgg ccgtcgccgc cgccaggccc
     2461 gccgcgccca agccggctgc ggccaagcc
//
LOCUS       DASVLN010000331         4533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_178629, whole genome shotgun sequence.
ACCESSION   DASVLN010000331 DASVLN010000000
VERSION     DASVLN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178629"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..893
                     /locus_tag="VF453_08045"
     CDS             198..893
                     /locus_tag="VF453_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEX7687642.1"
                     /translation="MSLPTLSLLETRILGVLVEKQHTVPDTYPLSLNALTAGCNQKTA
                     RDPVIAASEGDVLAALDTLRSLNLVFEGSGSRVPRYEHNTGRVLGVPSQSVALLATLM
                     LRGPQTAAELRQNSERLHRFADISSVEGFLDELATHEPPYVARLARAPGAREARWAHL
                     LSGTPAEVAPTAATGAVGTPLADAGLGLSEIAAMRAEQLRMNAEIGRLRAQVERLAKE
                     LGVDLDGDPPAAP"
     gene            complement(899..1240)
                     /locus_tag="VF453_08050"
     CDS             complement(899..1240)
                     /locus_tag="VF453_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687643.1"
                     /translation="MPFIRRNAEGVVESLHRHAEDGAEFLPDDHPEVRAFLGGPPAAD
                     GSQHGERFAELDADFVRVIEDVIDTLISKNVINITDLPSEAQTKLFGRKSFRERANRD
                     ALRLFGEGETD"
     gene            complement(1327..1959)
                     /locus_tag="VF453_08055"
     CDS             complement(1327..1959)
                     /locus_tag="VF453_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7687644.1"
                     /translation="MRSLYVLALAALIASPVLAEEAAKGPAKPDLGKGEEISKTVCAA
                     CHTADGSRGIPTNPILQGQHADYLVKQLTEFKAGKRDNPIMKPMASQLSDADMRNVAA
                     FYESKKAKPGQSKVKATVDLGQKIWRGGIAEKAVPACAGCHGPTGAGVPAQYPRLGGQ
                     HAEYVSAQLQLFRSGKRANSPQMATIAARMSDAEIAAVSDYAEGLNGPAH"
     gene            2022..2861
                     /gene="yihA"
                     /locus_tag="VF453_08060"
     CDS             2022..2861
                     /gene="yihA"
                     /locus_tag="VF453_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX7687645.1"
                     /translation="MTRPLPSRKPSPSRASAADKPASTKPAAAPAPGSAPAAPSPARQ
                     ALGWLHTTRFLTSASRIEQLPQGDMPEIAFVGRSNAGKSSAINTLAQQRQLAFASKTP
                     GRTQLINLFALGPKDAPEAYLADLPGYGYAAVDRGKKLVWQKVMADYLEVRRALLGVV
                     LMVDSRHGFTPLDQRLLDFVAQRVRTGEVKLLVLLTKADKLTKTEAKAAVDAAQEVLG
                     EFVTEASDIGVTPFSSLRRTGVEDVAETLRAWVDAAPERVEFELPPPSADGDVPLDES
                     PQG"
     gene            complement(2881..3510)
                     /locus_tag="VF453_08065"
     CDS             complement(2881..3510)
                     /locus_tag="VF453_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009981405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7687646.1"
                     /translation="MTSADLRRVFERAEPGARKAHKRQLMACALDCFERTGLEATTIE
                     DIRAAADSSIGSIYHHFGNKDGLVAALFFAALDDQAALVEPQIEAARDARDAVEASVR
                     SYLEWVTAQPRLARFVYRARASVAAGPHAQALADRNRARHQQLRRWLVRGVEEGAIRE
                     LPKETYVPLLIGQSEVYCRAWLSERVKGKPSDHAAVFAEAAWRSLATGS"
     gene            3623..4156
                     /locus_tag="VF453_08070"
     CDS             3623..4156
                     /locus_tag="VF453_08070"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEX7687647.1"
                     /translation="MSSDRPGARVALRTFVYRQVLNFWPCIRRSGGRVVALAPDFSRL
                     TVRLRLGWGTRNVVGTIFGGAMFSSTDPMYMLMLQRILGRDYVVWDKHGAVRFKRPAR
                     STLYAEFHVSPERRAEVVEAVRARGEGDFTWTILFKDARGIVHAEIDKTLYVADKAFY
                     KRKVAAREAAQGAAAAA"
     gene            complement(4272..>4533)
                     /locus_tag="VF453_08075"
     CDS             complement(4272..>4533)
                     /locus_tag="VF453_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX7687648.1"
                     /translation="SVVINKVLEGKGNFGFNAANDTYGDMIEMGILDPTKVTRTALQN
                     AASVASLMLTTECMVAEAPKEDAPAMPGGGMGGMGGMGGMDM"
BASE COUNT          615 a         1637 c         1601 g          680 t
ORIGIN      
        1 gacggtcgcg gcgcggtggt gacggagcgg ggtggcacgg cttcggatct tggggtggcg
       61 aaggcggatg gcgcgggatt gtccgcgcgc ccggccgcgc ggcccacccg ggccgacccg
      121 cgacgatgta accggatgca cgggacggcc gccgcgcgcg cgatactcgc gggaacgccc
      181 gctgccgacc ccgcaccatg tccctgccca cgctctcgct gctcgagacc cgcatcctcg
      241 gcgtcctcgt cgagaagcag cacaccgtgc ccgacacgta tccgctcagc ctgaacgcgc
      301 tgaccgccgg ctgcaaccag aagacggcac gcgatcccgt gatcgccgcg agcgagggcg
      361 acgtgctcgc cgcgctcgac acgctgcggt cgctcaacct ggtgttcgag ggctcgggca
      421 gccgcgtgcc gcgctacgag cacaacaccg ggcgcgtgct gggcgtgccg agccagagcg
      481 tcgcgctgct cgccacgctg atgctgcgcg gcccgcagac cgccgcggag ctgcggcaga
      541 acagcgagcg gctgcatcgt ttcgcggaca tctcgtcggt cgaaggcttc ctcgacgagc
      601 tcgcgacgca cgagccgccc tacgtcgccc gcctcgcgcg cgcgcccggg gcgcgcgagg
      661 cgcgctgggc gcacctgctc tcggggacgc cggcggaagt cgcgcccacg gccgccaccg
      721 gcgccgtcgg cacgccgctg gcggacgcgg gactggggtt gtcggagatc gcggcgatgc
      781 gcgccgagca gctgcgcatg aatgccgaga tcgggcgcct gcgcgcgcag gtcgagcggc
      841 tcgcgaagga gctgggcgtc gacctcgacg gcgatccgcc cgccgcccct tgaggccttc
      901 agtcggtctc gccctcgccg aacagccgca gcgcgtcgcg gttggcgcgc tcgcggaagc
      961 tcttgcggcc gaacagcttg gtctgcgcct cggacggcag gtcggtgatg ttgatgacgt
     1021 tcttcgagat gagcgtgtcg atcacgtcct cgatcacgcg cacgaagtcg gcgtcgagct
     1081 cggcgaagcg ctcgccgtgc tgcgagccgt cggccgcggg cggcccgccg aggaaggcgc
     1141 gcacctcggg atggtcgtcg ggcaggaact cggcgccgtc ctcggcgtgg cggtgcaggc
     1201 tctcgacgac gccctcggcg ttgcggcgga tgaacggcat cgggcgatga tagtcccgcg
     1261 cgcgcgggcg taccgccggc gccggccgcg aagccggaac ctcttcacgc gcccgcgcgc
     1321 ggcgtgtcag tgcgcagggc cgttcaggcc ttcggcgtag tccgagaccg ccgcgatctc
     1381 ggcatcgctc atgcgcgccg cgatggtggc catctgcggg ctgttggcgc gcttgccgga
     1441 gcgaaacagc tgcagctgcg ccgacacgta ctccgcgtgc tggccgccca ggcgcggata
     1501 ttgcgcgggg acgccggcgc cggtcgggcc gtggcagccc gcgcacgcgg gcaccgcctt
     1561 ctccgcgatg ccgccgcgcc agatcttctg gcccaggtcg acggtcgcct tgaccttgga
     1621 ctggcccggc ttggccttct tcgactcgta gaacgcggcg acgttgcgca tgtcggcgtc
     1681 ggacagctgc gaggccatcg gcttcatgat cgggttgtcc cgcttgcccg ccttgaactc
     1741 cgtcagctgc ttgaccaggt agtcggcatg ctggccttgc aggatgggat tcgtcgggat
     1801 gccgcggcta ccgtcggcgg tatggcacgc ggcgcacacc gtcttcgaga tctcctcgcc
     1861 cttgcccagg tccggcttgg ccgggccctt cgcagcttct tccgccagca ccggggaggc
     1921 gatcagcgcc gccagggcaa gcacgtacag ggacctcatc gctagcaacc cttctcgagt
     1981 cttggatgga gagagcaaac cggtcgattt tataatgcac catgacccgt ccgttgccct
     2041 cgcgcaaacc ctcaccctcg cgcgcgtcgg cggcggacaa gcccgcctcg acgaagcccg
     2101 cggccgcccc ggctccggga tccgcgccgg ccgccccgtc gccggccagg caggccctcg
     2161 gctggctgca caccacgcgg ttcctgacct cggccagccg catcgagcag ctgccgcagg
     2221 gcgacatgcc cgagatcgcg ttcgtcggcc gctccaacgc gggcaagtcc tcggcgatca
     2281 acaccctcgc gcagcagcgc cagctcgcct tcgcctcgaa gacgccgggc cgcacgcaac
     2341 tgatcaacct gttcgccctc ggcccgaagg acgcgcccga ggcctacctc gccgaccttc
     2401 cgggctacgg ctacgccgcc gtcgaccgcg gcaagaagct ggtgtggcag aaggtgatgg
     2461 ccgactacct cgaggtgcgc cgcgccctgc tgggagtcgt gctgatggtg gacagccgcc
     2521 acggtttcac gccactcgac cagcggctgc tcgatttcgt cgcccaacgc gtgcgcacgg
     2581 gcgaggtcaa gctgctggtg ctgctcacga aggccgacaa gctcacgaag accgaggcga
     2641 aggctgccgt cgatgcggcg caggaggtcc tgggcgagtt cgtcaccgag gcctcggaca
     2701 tcggcgtgac gccgttctcc tcgctgcgcc gcaccggcgt cgaggacgtc gccgagaccc
     2761 tgcgcgcctg ggtcgacgcg gcgccggaac gggtggagtt cgagctgccg ccgccctcgg
     2821 cggacggcga cgtgccgctc gacgagtccc cacagggctg aagcgcgccc cgcgcctcgc
     2881 tcaggacccc gtcgccagcg accgccacgc cgcctcggcg aacaccgccg cgtggtcgga
     2941 cggcttgccc ttgacgcgct ccgacagcca ggcgcggcag tagacctcgg actggccgat
     3001 cagcagcggc acgtaggtct ccttgggcag ctcgcggatc gccccttcct cgaccccgcg
     3061 cacgagccag cgccgcagtt gctggtggcg cgcgcggttg cggtcggcga gtgcctgcgc
     3121 gtgcggcccg gcggcgaccg atgcgcgggc gcggtagacg aagcgcgcca gccgcggctg
     3181 cgccgtcacc cactccaggt agctgcgcac gctcgcctcg accgcgtcgc gcgcgtcgcg
     3241 ggcggcctcg atctgcggtt cgacgagcgc cgcctggtcg tccagcgccg cgaagaacag
     3301 cgcggcgacg aggccgtcct tgttgccgaa gtggtggtag atcgagccga tgctcgagtc
     3361 ggccgcggcg cggatgtcct cgatcgtcgt cgcctccagg cccgtgcgct cgaagcagtc
     3421 gagcgcgcac gccatcagct ggcgcttgtg cgccttgcgc gcgccgggct cggcgcgttc
     3481 gaagacgcgg cggaggtctg cggaggtcat gtccgccggg agtataggca ccggacgccg
     3541 cgctggattg aaggcaagtt ctagaactat attctgtaat gcgctagagt cgttccgtcg
     3601 tgtcttgccg caggtggaat ccatgagctc cgatcgcccg ggcgcgcgcg tcgcgctgcg
     3661 caccttcgtc taccgccagg tgctgaactt ctggccgtgc atccgccgat ccggtgggcg
     3721 cgtggtcgcg ctcgcgcccg acttctcccg cctgaccgtg cgcctgcgcc tgggctgggg
     3781 cacgcgcaac gtcgtcggca cgatcttcgg cggcgcgatg ttctcgtcga ccgacccgat
     3841 gtacatgctg atgctgcagc gcatcctcgg ccgcgactac gtcgtctggg acaagcacgg
     3901 cgccgtgcgc ttcaagcgcc cggcgcgctc gacgctgtac gccgagttcc atgtcagccc
     3961 cgagcgccgc gccgaggtgg tcgaggcggt gcgcgcgcgg ggcgagggcg atttcacgtg
     4021 gaccatcctg ttcaaggacg cccggggcat cgtgcacgcg gagatcgaca agacgctcta
     4081 cgtcgccgac aaggcgttct acaagcgcaa ggtggccgcg cgcgaggcgg cgcaaggcgc
     4141 ggccgccgcg gcctgatcgc tgccggtcat cgcgggatcg agcgcggcgc caaagaaaaa
     4201 gggactgccg aagcagtccc cttgcgacgt gcgcggtgcg cggcgcgggc ggcgagccgc
     4261 ccgcttcgga cttacatgtc catgccgccc atgccgccca tgccacccat gccgccgccg
     4321 ggcatcgccg gggcgtcttc cttcggggcc tcggcgacca tgcactcggt cgtcagcatc
     4381 agcgaggcga ccgacgcggc gttctgcagc gcggtgcggg tcaccttggt cggatccagg
     4441 atgcccatct cgatcatgtc gccgtaggtg tcgttggcgg cgttgaagcc gaagttgccc
     4501 ttgccttcca gcaccttgtt gatcacgacc gag
//
LOCUS       DASVLN010000332        15992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_178723, whole genome shotgun sequence.
ACCESSION   DASVLN010000332 DASVLN010000000
VERSION     DASVLN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15992
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178723"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VF453_08080"
     CDS             complement(<1..467)
                     /locus_tag="VF453_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7687649.1"
                     /translation="MSKTRVVVVDDSALVRSLLTEIINRQPDMECIGSASDPFAAREM
                     IRNLNPDVITLDVEMPRMDGIDFLSKLMRLRPMPVVMVSTLTERDAEVTLKALELGAI
                     DFVAKPKIGVADGLKQLAEEITDKIRTASKARVAKHVPPAGAAAAAGAPAPVRA"
     gene            complement(495..1166)
                     /gene="cheD"
                     /locus_tag="VF453_08085"
     CDS             complement(495..1166)
                     /gene="cheD"
                     /locus_tag="VF453_08085"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HEX7687650.1"
                     /translation="MTPVPGARPPATTLRATSGPGSRLERLKSAARKPGEASFFFYDA
                     HFQNEAVKVLPGEYYVDNEDLLVMTTLGSCIAACLYDRQARIGGMNHFMLPEGNGDSG
                     RYGTYAMELLINEMMKRGATRLTMEAKIFGGGAVISGMNSINVGERNTKFVMDFLNTE
                     RIPIVSKDVLEIYPRKVCFLPASGKAMVKRLAPTNTDALVQQDRVAAQKAAPATTGGG
                     SIDLF"
     gene            complement(1234..2082)
                     /locus_tag="VF453_08090"
     CDS             complement(1234..2082)
                     /locus_tag="VF453_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX7687651.1"
                     /translation="MSTDLATLASPDAAAHEFTFSNGDFQRVQKLIYQRAGISLHDGK
                     RAMVYSRLSRRLRETGDRSFADYLDGLERATGAAGDVEWQEFINCLTTNLTSFFREEH
                     HFHALAEALVPLAGKPIRMWCNAASTGEEPYSIAMTLVEAGCKQARLLATDIDTKVLA
                     TAQRGVYAADARGLSTQRLQRHMLRGKGANAGFMRVKPELQKLVEFRTFNLMATAWSL
                     GEPFDMVFCRNVMIYFDHATQRKVLEHIHEVMKPGGLLFVGHSENFTDSRDLFQLRGK
                     TIYQRV"
     gene            2303..3031
                     /locus_tag="VF453_08095"
     CDS             2303..3031
                     /locus_tag="VF453_08095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7687652.1"
                     /translation="MNSKPEFPLPASEALRLEQALALLAQSGHGRPTGVEGSPGWLQS
                     LIDALCDLSSRDGLTGLANRRYFENALARESDRVARSGEPALLLMLDIDHFKRINDHH
                     GHVAGDLVIKAVADALTATVRPMDLVARIGGEEFAIILPSCPPAFGAAVAERLRGCVE
                     ERAIHLVGGQTLGCTISIGGAYAPQWVRSTPSVWMERADLQLYRAKSEGRNRTCLEPT
                     PVSVVSAEEKGLLFASSIFGEDLS"
     gene            3028..3822
                     /locus_tag="VF453_08100"
     CDS             3028..3822
                     /locus_tag="VF453_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEX7687653.1"
                     /translation="MTTTTTARPAVAGRRARITAITSGKGGVGKTFLSANLAAALARQ
                     GERVLVLDADLGLANLDVVLNLFPKITLHDVFTGKSTLEHAILEAPGGFSVLLAGSGM
                     VEYSRLTPEVRQQLVDVIEQVVPRFDRILLDTGAGISDVVLYTVSLADEVLIVATPEP
                     TSLTDAYATIKVLATTQGRRDLRVLVNQVSKPGEGRVIRGQLQQVVDRFVSPGLEVPV
                     KLEFVGEVPSDAAVRDAVKKRQLLLECLPGSNAAQAVVAGATRLRA"
     gene            3892..4344
                     /locus_tag="VF453_08105"
     CDS             3892..4344
                     /locus_tag="VF453_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEX7687654.1"
                     /translation="MTTPPDAASGDATDPRDAPSPTSLFEQLGGEPGVRALVDRFYDL
                     MELEPEFADLRGLHPTTTEGSRDKLFWFLCGWLGGPDHYIERFGHPRLRMRHFPYAIG
                     VSQRDQWITCMGLAMAEIGEAGGFTPAIQQRLLQAFAQTANHMRNTPF"
     gene            4745..5191
                     /locus_tag="VF453_08110"
     CDS             4745..5191
                     /locus_tag="VF453_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687655.1"
                     /translation="MLEAPSASDVATPPAPEAQPNEQQAERLTREIVARFACDPVAFA
                     TSYQVEPSAFVRDYLQSLNKRLSGFATAPREAAEPSRRLATLGFDQLADVPGGLPVDA
                     DLWGPENAPRMGAAAVVSVVASVATVVTSVVQAYTALTSLIKHKPL"
     gene            5191..5739
                     /locus_tag="VF453_08115"
     CDS             5191..5739
                     /locus_tag="VF453_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687656.1"
                     /translation="MALAAGIGADASAPIPCERMCFTNPGVNMHTVDELIPPRNREQR
                     PASAAQIGDSVPVMALRAGSALDAASSHECARLLRRFVHDPVETASEARIDSAELMRR
                     YRAGVDRRIAEAGVTRDGAARRPLATLGPDAPCEVDLPGGAGQSRPIQMGIASLSSAM
                     TVVTVVSAALSAISSLLRHKPV"
     gene            5863..6933
                     /locus_tag="VF453_08120"
     CDS             5863..6933
                     /locus_tag="VF453_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7687657.1"
                     /translation="MKPYEEGRSGPQQERFLLPMMFQALVVESTTRCNAKCGMCYQGA
                     GPKGSDYLGDAALSVDDIKQVLRSGLSIPSLTRRFHMAGGEAFIKVDECVEIFSYASA
                     LGWREVSCTTNAYWAADIAKAHGLARRLRDAGLQRAEISWDHWHGPHIPAHAINNCLH
                     AMEANDIQTNLRLLTSRSHSAEEALAALSPAALAKASVITSAPVFQVGRAMELPAEDI
                     YASDQNATCHSALNLTVNARGFVYPCCAGFDQTNSELFGSIRERDIADIAADINRSLL
                     VRLVVFEGINSLRPILQLLGEELKDQYSSICGMCFEVFSNPERVRKLTDFFQQIEAEA
                     LREALQKRKSDSSDADELEVAQ"
     gene            6930..7868
                     /locus_tag="VF453_08125"
     CDS             6930..7868
                     /locus_tag="VF453_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687658.1"
                     /translation="MTDALARAEMSFVDNLRRIAVIAQVDDSSGVPTLLPSVDALLRW
                     VAEYNAAFVQANERWCAGRLHSFHHRQVTLAGRHLFLAPDDILRIGRYLQKIGVSLAV
                     SVPIQEMSRYPLVLPTLRSELPGLYVQIECAEASAGVDADVDAATTAAAAAEPQPEAK
                     RGLSSESRRALEAHVDSGGSVNLLSSIAELRHAGLAQHEGLNRAGFSVARPAPRGTRP
                     MHRIHRVSPCSDYLGWHVDAAGDIFPCIGMVGHAPARLGNIAGRFVDALHALAGTRDA
                     MVGLAERGPDFLAPPASVPFDLCALHRDAVTDLESI"
     gene            7906..8964
                     /locus_tag="VF453_08130"
     CDS             7906..8964
                     /locus_tag="VF453_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7687659.1"
                     /translation="MGMLLVVIATCAIVGKAAAPRSRVTTAHPRVRDIERRIEAAGTM
                     RPVSVVHVGALVSGVVVNATVEAGDHVQRGQRIATIDQEPYRLQLERAHALARAAQRR
                     AAQARSALYRQQDLQRAGFIAPAALDASEAAAREADEDVRVAFADVETARINLAHCEI
                     DSPISGIVLTKEIAAGQSVASSFQVPDLFTIVSRLDRLEVVAMFAEADLGAVTPHAEV
                     KLQVPAFPDRNFTATVERVLDTPESRQGVVMFPVVLGLANAGGLLRPGMTAYAELRVA
                     SVRGALTVPHKAVAFARSRDRAAHQEASGPRHLYVLRNGAVTPITVQFGASDELGTQV
                     LPVDRLDPHDDVVLTDSP"
     gene            8972..9451
                     /locus_tag="VF453_08135"
     CDS             8972..9451
                     /locus_tag="VF453_08135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7687660.1"
                     /translation="MYLIPMSPADIPAYLRGFADWHAQALLDVAIGDGRDIDELHARA
                     QAQIQPQFGENGLPVGSTIFDLCTNDANERVGVLWAGGADFGFGPLFYIHDLRIHAPF
                     RRRGFAREALDEVYQIAKERGGVVGVALSVLAGNAAARELYRASSFRVLSEVMVRAL"
     gene            9537..9938
                     /locus_tag="VF453_08140"
     CDS             9537..9938
                     /locus_tag="VF453_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687661.1"
                     /translation="MKSAITLIRSVSVALLAYVLIDGPLGMLLQSALPGETDGRGHSV
                     GLPLMLGDLTIHGIALFAAGALAASTTSESSPRGAFVASAAVFAFVLGLTLALWDAEP
                     AWFDIACLLMTPPFQLLASAWHRAAGKDPHA"
     gene            9935..10846
                     /locus_tag="VF453_08145"
     CDS             9935..10846
                     /locus_tag="VF453_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687662.1"
                     /translation="MSNRMSPDDPDRGIATMMSFSSSQSSSPPIECSRALPSPAGRGP
                     STWQLLRAESGRQRTLLARYPLEAAVTVVGFACMFLIFAAIGTVSTGPYAIFSGSLQS
                     LALIYVLWTMSAATVVAGASQVSADAAIGVLENLFLSCSPIIRILSARTAARICLSVP
                     MGLVLLAAFCLGTAWAPPPVVVAAMAVAALACGLTGLGLGFAFAGAALVTKRIVVLGM
                     PVNFLCMLAFMSQHAAAGDRLDNPVLYLPFVAAATLVRRAVELGRFEPVLVAQALVGV
                     LVWVPLGRLVLARCVQACRRVGSVNLY"
     gene            10849..11535
                     /locus_tag="VF453_08150"
     CDS             10849..11535
                     /locus_tag="VF453_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7687663.1"
                     /translation="MSTSEIFAIDAAGLSRIFHPPGASRVEALPPRDLRVRAGESVAL
                     TGASGCGKSTLLGLLSLMEAPSAGVLRLFGTDVSVLGRSARARFRQQHIGVVFQNFCL
                     LKDRSVLANVELPLAYAELSPAKRRERALDWLGRVGLGNLAHRHPTELSGGQQQRVAI
                     ARAMATGPRLLLADEPTGSLDRDTGERVSDDLLRFCADTGATCVIATHDPLLAARCAR
                     VERMDRDACR"
     gene            11522..12739
                     /locus_tag="VF453_08155"
     CDS             11522..12739
                     /locus_tag="VF453_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7687664.1"
                     /translation="MRAADFAREVSGALLRDRLRAALTVAGIAVGSFAVAALLSLGAG
                     VQGAIGDSLKGLGENLVIVTPERSRSGALAGAAAQAAALTEADVRELRRLDAVQAVSG
                     VVTARVSAAAGPESITTTCLGVDGEYADVVRLELDEGGAARDELGASPWPVAVLGASV
                     AARLFPDGDGVGRRIELSGHRVQVVGVARERGRDAGGADEDDFVLVPRAFAREQLVAH
                     PTPDGIDAVFALAVPGEVDDAVEEASEALGRRRPGVELKDALAVNSMRGLMSASVAAA
                     RLLTVLMGGMAAISVLVGGVGIVNVMLANVADRRLEIGLKLALGAPPVVIGLEFLAGA
                     VAFALAGAVAGLLGAWMLLRLLSMAQVADVSLTPAATALATALAGIVGVVAGAYPAVQ
                     AARLQPREILRDA"
     gene            12732..13454
                     /locus_tag="VF453_08160"
     CDS             12732..13454
                     /locus_tag="VF453_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7687665.1"
                     /translation="MLDEPTALALADVDKAFAARGRRRQVLAGVHLRAAAGELVAITG
                     PNGAGKSTVLRLVAGLLLPDAGTVRVADRRGCLCDPAGQPGLVSAVFDGSRGLYWKLT
                     VAENLRYFASLDGLSGPAAMAAAAPWLERFGLEARQDDLVQNLSKGTQQKVSIVRALA
                     LAKPVLLFDEPTVLLDEDACWQLAQSLKSLCRQGHTVVVATHDRDFVGLTQARQVRVV
                     PEGGLVAAAVAPVRTEAATLLH"
     gene            13715..14818
                     /locus_tag="VF453_08165"
     CDS             13715..14818
                     /locus_tag="VF453_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7687666.1"
                     /translation="MLPLQEVASQIKPGSPLAWGIRDSNGKLLLARGHRVADAAMLET
                     LLARGMFVDADEMRAAGRSGAPPKEGFFGRWRLSSARLNTLLTSPPAQLRAAMDEVVG
                     VLMALAERYPDKVLFQILRHDQTRLQTYGVSHSLHCAAVCCLTGKRMGWDEAQRRTLV
                     SAAMSMNLSMIELQGRLAVQGARPTPEQRAAIEAHPHKSAEMLRASGVDDALWLAAVE
                     QHHELVDGSGYPLRRTDVAEFAQALHYVDVFTAKLSARSTRGPLPANVAAREIFTASG
                     GHPLAAALIKEFGIYPPGCFVRLASGETAIVLHRGATVNTPIVACLTKPNGQPMMRPV
                     QRETTSRDAAVVAIVPDGDVMVRVPWETLYSDD"
     gene            complement(14924..>15992)
                     /locus_tag="VF453_08170"
     CDS             complement(14924..>15992)
                     /locus_tag="VF453_08170"
                     /inference="COORDINATES: protein motif:HMM:NF020467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1839 family protein"
                     /protein_id="HEX7687667.1"
                     /translation="GLSAAGWTPHALHGPDATWLEKNCYVDLWIELLSALELDPHALW
                     PCVTAIDFEGDQWTFFKPPHAELRALYGIDVQELTVWRPLAEHAIEHLAAGRLLSVEV
                     DAFWLPDTAGTDYHRQHAKTTIVLNEVDLAARRLRYFHNAGYFEASGDDCQHLLGLPD
                     ALSDLEAIADWRGTRVATIGAATLALDEGRLPLFAEFIRTDRLIARSTSELRLLAQRH
                     LADHLAFRPRDNPVRRFARRLVDDLPLLRDHGLDYYHRWAFGTVRQLGAAFDLAARGL
                     AWQALPGSEGDAALQRAGEAFDGLARDAKSLILKLARAVSSPRATEVGTLLEGMAAAW
                     DEGMAALDEANAVDWGQRHPE"
BASE COUNT         2237 a         5520 c         5611 g         2624 t
ORIGIN      
        1 gcgcgcacgg gcgccggggc gccggcggcc gccgcagcgc ccgcgggcgg cacgtgcttg
       61 gccacgcgcg ccttcgaggc ggtgcggatc ttgtcggtga tctcctcggc cagctgcttc
      121 aggccgtcgg ccacgccgat cttgggcttg gcgacgaagt cgatcgcgcc cagctccagc
      181 gccttgagcg tcacctcggc gtcgcgctcg gtcagcgtcg agaccatcac gaccggcatc
      241 ggccgcagcc gcatcagctt ggagaggaag tcgatgccgt ccatgcgcgg catctcgacg
      301 tcgagggtga tgacgtcggg gttgaggttg cggatcatct cgcgcgcggc gaacgggtcg
      361 ctcgccgagc cgatgcattc catgtcgggc tggcggttga tgatctccgt gagcaggctg
      421 cgcacgaggg ccgaatcgtc gacgacgacc acgcgggtct tggacatgtg ttggtctccc
      481 cggggtgggc cgtctcagaa caggtcgatc gagccgccgc ccgtcgtcgc gggagccgcc
      541 ttctgggcgg ccacgcgatc ctgttgcacg agcgcgtcgg tgttggtggg cgcgaggcgc
      601 ttgaccatcg ccttgccgct ggccggcagg aagcagacct tgcgcgggta gatctcgagc
      661 acgtccttgg agacgatggg gatgcgctcc gtgttgagga agtccatgac gaacttcgtg
      721 ttgcgctcgc cgacgttgat cgagttcatg cccgagatca cggcgccgcc gccgaagatc
      781 ttggcctcca tcgtcaggcg cgtcgcgccg cgcttcatca tctcgttgat cagcagttcc
      841 atggcgtacg tgccgtagcg gcccgagtcg ccgttgccct cgggcagcat gaagtggttc
      901 atgcccccga tgcgcgcctg gcggtcgtac aggcaggcgg cgatgcacga gccgagcgtc
      961 gtcatcacca gcaggtcctc gttgtcgacg tagtactcgc cggggagcac cttcacggcc
     1021 tcgttctgga agtgcgcgtc gtagaagaag aacgacgcct cgcccggctt gcgcgcggcc
     1081 gacttcaggc gctcgaggcg agaaccgggg ccggaggtcg cgcgcagcgt cgtcgccggc
     1141 gggcgtgcgc ccgggacggg ggtcatggtt tggagggcca ttgaatgcag acctgatggg
     1201 gtgtttcgga ttcgtcgggg gcgtccgcgg agctcagacg cgctggtaga tggtcttgcc
     1261 gcgcagttgg aacaggtcgc gcgagtcggt gaagttctcc gagtggccga cgaacagcag
     1321 gccgcccggc ttcatcacct cgtggatgtg ctcgagcacc ttgcgctggg tggcgtggtc
     1381 gaaatagatc atcacgttgc ggcagaacac catgtcgaag ggctcgccga gcgaccacgc
     1441 ggtcgccatc aggttgaacg tgcggaactc gacgagcttc tgcagctcgg gcttgacgcg
     1501 catgaagccg gcgttcgcgc ccttgccgcg cagcatgtgg cgttgcaggc gctgcgtcga
     1561 caggccccgc gcatcggccg cgtagacgcc gcgctgggcg gtggccagca ccttggtgtc
     1621 gatgtcggtg gcgagcagcc gggcctgctt gcagccggcc tcgaccagcg tcatcgcgat
     1681 cgagtagggc tcctcgcccg tcgaggcggc gttgcaccac atgcggatcg gcttgccggc
     1741 cagcggcacg agggcctcgg ccagcgcgtg gaagtggtgc tcctcgcgga agaacgaggt
     1801 caggttggtc gtcaggcagt tgatgaactc ctgccactcg acgtcgccgg ccgcgccggt
     1861 cgcgcgctcc aggccgtcga ggtagtcggc gaagctgcgg tcgccggtct cgcgcaggcg
     1921 gcgcgacagc cggctgtaga ccatggcccg cttgccgtcg tgcaggctga tcccggcgcg
     1981 ctggtagatc agcttctgca cgcgctggaa gtccccgttg ctgaacgtga actcgtgggc
     2041 ggcggcgtcg ggcgaggcga gggtggcgag gtcggtgctc atctttgggc gggcttcccg
     2101 cgtggaacct ggattcggta cgggtttatc ggacgatgcc gcccagtctc aagactctcg
     2161 ccctggcgcg gcgagccgag atggccgtga aatgcggcgc ttttcgcgtt tcccggccgg
     2221 ctagggcggg cgttagactg accccgcacc agggcgaccg tccgtcgcca tcgttcctgt
     2281 cgatccattg acagctttcc ccgtgaactc gaagcccgaa ttcccgctgc ccgcgtccga
     2341 ggcgctccgc ctggagcagg ccctcgcgtt gctcgcgcag tcggggcacg gccgtccgac
     2401 gggcgtcgag ggcagccccg gctggctcca atcgctgatc gacgcgctgt gcgacctctc
     2461 cagccgcgat ggcctgaccg gcctcgccaa ccgccggtat ttcgagaacg cgctcgcgcg
     2521 cgagtccgac cgcgtcgcgc gttccgggga gcccgcgctg ctgctgatgc tcgacatcga
     2581 tcacttcaag cgcatcaacg accaccacgg ccacgtcgcc ggcgacctcg tcatcaaggc
     2641 ggtcgccgac gcgctcaccg ccaccgtgcg gccgatggac ctcgtcgcgc gcatcggcgg
     2701 cgaggagttc gcgatcatcc tgcccagctg tccgccggcc ttcggtgccg cggtggccga
     2761 gcgcctgcgc ggctgcgtcg aggagcgcgc gatccatctc gtcggcggcc agacgctcgg
     2821 ctgcacgatc agcatcggcg gcgcctatgc gccccagtgg gtgcgctcga cgccctccgt
     2881 atggatggag cgcgccgacc tgcagctcta ccgcgccaaa tccgagggcc gcaaccgcac
     2941 ctgcctcgag ccgacgcccg tttccgtcgt ctcggcggag gagaagggcc tgctgttcgc
     3001 ctcgtccatc ttcggggaag acctttcatg acgaccacga ccacggctcg ccccgccgtc
     3061 gccggccgcc gcgcacgcat caccgccatc accagcggca aggggggcgt gggcaagacc
     3121 ttcctctcgg ccaacctcgc cgcggcgctc gcgcgccagg gcgagcgcgt gctggtgctc
     3181 gatgccgacc tgggtctcgc caacctggac gtcgtgctca acctgttccc caagatcacg
     3241 ttgcacgacg tcttcaccgg caagagcacg ctcgagcatg cgatcctcga ggcgccgggc
     3301 ggcttctcgg tgctgctggc cggctcgggg atggtcgagt actcgcgcct gacgcccgag
     3361 gtgcgccagc agctcgtcga cgtcatcgag caggtggtgc cgcgcttcga ccgcatcctg
     3421 ctggataccg gggccggtat ctcggacgtc gtcctctaca cggtgtccct ggccgatgag
     3481 gtgctcatcg tcgcgacgcc ggagcccacg tcgctcaccg acgcctatgc caccatcaag
     3541 gtgctcgcga ccacgcaggg ccggcgcgac cttcgcgtgc tggtcaacca ggtgagcaag
     3601 ccgggcgagg ggcgcgtgat ccgcggccag ttgcagcagg tcgtcgaccg cttcgtgagc
     3661 ccggggctgg aggtgcccgt gaagctggag ttcgtgggcg aggtgccgtc cgatgccgcc
     3721 gtgcgcgacg ccgtgaagaa gcgtcagctg ctgctcgagt gcctgcccgg ctcgaacgcg
     3781 gcgcaggcgg tggttgccgg cgccacgcgc ctgcgcgcct gacccgcgcg cggccctggc
     3841 gccgcagcct cggcccggca aaggccatcg tcccgcggga caatctctcc catgaccacc
     3901 ccccccgacg ccgcctccgg cgacgccacc gatccccgcg acgcgccgag tccgacatcg
     3961 ctgttcgagc agctcggcgg cgagcccggc gtgcgcgcgc tggtcgaccg cttctacgac
     4021 ctcatggagc tcgagcccga gtttgccgac ctgcgcggtc tgcatccgac gacgaccgag
     4081 ggctcgcgcg acaagctgtt ctggttcctc tgcggctggc tgggcggccc cgaccactac
     4141 atcgagcgct tcggccaccc gcgcctgcgc atgcgccatt tcccgtacgc gatcggggtc
     4201 tcccagcgcg accagtggat cacctgcatg ggcctggcca tggcggagat cggcgaagcg
     4261 ggcggattca cgccggcgat ccagcagcgg ctgctgcagg cattcgcgca gacggcaaat
     4321 cacatgagaa ataccccgtt ttgacgcggg cgacctgcgc cgtcctggcg cagactgcat
     4381 tcatgcggcc ggcgcgatcc gcctgccaca gatggttcga ggggatgtat ccccaacttg
     4441 catcggccca gccctttact gtgcgtcgaa actaagaaat acttagttca tgctcagtca
     4501 acgccgctga tgcccaccgc gatccgacac cgtgcgtgcc ggacgccatc ccctcccacg
     4561 cgcgaccggt cgcgcgtctg ccagcctgcg gtgccgacac gctcgcaccg tcagccatcc
     4621 cccggagcgg ccctcccatc ctcgttcgct gtcgtcacgc acgtggcgac ggcatgcgcc
     4681 gctccgccct tcatcgttcg tccttggaca tccgtttctt ccttccatca tcaagggagt
     4741 caagatgctt gaagccccgt ccgcgtccga tgtcgcgacg cctcctgcgc ccgaggcgca
     4801 gccgaacgag cagcaggccg agcgcctcac ccgcgagatc gtcgcccgct tcgcgtgcga
     4861 tcccgtcgcc ttcgccactt cgtaccaggt cgagccctcg gccttcgtcc gcgactacct
     4921 gcagagcctg aacaagcgcc tgagcggctt cgcgacagcc ccgcgcgaag cggcggagcc
     4981 gtcgcgccgg ctggcgacgc tgggcttcga ccaactggcc gacgtgcccg gcggcttgcc
     5041 ggtcgatgcc gacctctggg ggcccgagaa cgcgccccgg atgggggcgg ctgccgtcgt
     5101 gagtgtggtc gcgtccgtcg ccaccgtggt gacgtccgtg gtgcaggcgt ataccgccct
     5161 caccagcctg atcaagcaca agccgctctg atggcgctcg ccgccgggat cggcgcggat
     5221 gcgtctgcgc cgatcccctg tgaacggatg tgctttacga atccaggagt caacatgcac
     5281 acagtcgatg aactgattcc cccgcggaat cgcgaacagc gtccggccag cgcggcgcag
     5341 atcggggaca gcgtccccgt catggccctg cgcgccgggt cggcgctcga cgcggcgagc
     5401 tcgcatgaat gcgcgcggct gctgcggcgc ttcgtccacg atccggtcga gaccgccagc
     5461 gaagcacgca tcgattccgc cgagttgatg cggcgctacc gcgcgggcgt cgatcgcagg
     5521 atcgccgagg caggtgtcac acgcgacggg gccgcgcgcc gacccctggc caccctgggc
     5581 ccggatgcgc cctgcgaggt cgacctcccc ggaggcgcag gccagtcgcg ccccatccag
     5641 atggggatcg cctcgctgtc ctccgcgatg acggtggtga ccgtcgtgtc tgcggccctc
     5701 tccgcaatca gcagcctgtt gaggcacaag ccggtgtagt ccgtcgcggg cccttgtccg
     5761 agggtgtccc tgccgacgcg gcgaacgcgg cggcgtgtgg ggacggcggg atccttgccg
     5821 gggtcggcgg ctgcgttcaa gaagcttgca ggaggagagt ccatgaaacc gtacgaagag
     5881 ggccgaagcg ggccccagca ggagcgcttt ctcctgccca tgatgttcca ggcgctggtc
     5941 gtggaatcga cgacccggtg caacgcgaaa tgcggcatgt gctaccaagg tgcgggcccc
     6001 aagggctccg actatctcgg cgacgcggcg ttgagcgtcg acgacatcaa gcaggtcctg
     6061 cgatccgggc tgtccatccc ctcgctcacc aggcgcttcc acatggccgg cggagaggcc
     6121 ttcatcaagg tcgacgagtg cgtcgagatc ttctcgtatg catccgccct gggatggcgc
     6181 gaggtttcct gcacgaccaa cgcctactgg gccgcggaca tcgccaaggc ccacggactt
     6241 gccaggcgct tgcgcgatgc cggcctgcag cgggcggaga tcagctggga ccactggcat
     6301 ggcccccaca tcccggccca cgcgatcaac aactgcctgc acgcgatgga ggccaacgac
     6361 atccagacca atctccggct gctcacctcc cggtcgcatt cggccgagga ggcgctggcc
     6421 gccctgtcgc cggctgcgct ggcaaaggcc tccgtcatca cgtcggcgcc ggtattccag
     6481 gtcggacgcg cgatggagct gcccgccgag gacatctacg cgagcgacca gaacgccacc
     6541 tgccactcgg cgctgaacct gaccgtcaat gcccgtggct tcgtgtatcc ctgctgcgcg
     6601 ggcttcgacc agacgaactc cgagctgttc ggcagcatcc gggagcgcga cattgccgac
     6661 attgccgcgg acatcaaccg ctcgctgctc gtgcgcctcg tggtgttcga gggaatcaac
     6721 tccttgcggc cgatcctgca actgctgggc gaggaattga aggatcagta cagctccatc
     6781 tgcggcatgt gcttcgaggt gttttcgaac cccgagcgtg tgcgcaagct caccgacttc
     6841 ttccagcaga tcgaagccga ggcgttgcgg gaggccttgc agaagcggaa gtcggattct
     6901 tccgacgcgg acgagctgga ggtcgcccaa tgacggatgc gctcgcaagg gcagagatga
     6961 gcttcgtcga caacctgcgc cggatcgctg tcattgcgca ggtcgacgat tcctccggcg
     7021 tcccgacttt gctgccttca gtcgacgcct tgctgcgctg ggtcgcggag tacaacgcgg
     7081 ctttcgtgca ggccaacgag cggtggtgcg ccggccgcct gcattccttc catcatcgac
     7141 aggtgaccct ggccggacgt catctgttcc tggcgcccga cgacatcctg cgaatcggcc
     7201 ggtacctgca gaagatcggc gtctcactgg ccgtcagcgt accgatccag gagatgagcc
     7261 ggtaccctct cgtgctgccg accttgcgca gcgaactgcc aggcctctac gtccagatcg
     7321 aatgcgcgga ggcctcggcc ggtgtggacg ccgacgtcga tgccgcaacc accgccgcag
     7381 ccgcagccga accccagccc gaagccaagc gcggcctgtc ttcggaatcc cggcgagcac
     7441 tcgaggcgca cgtggacagt ggcggcagcg tcaatctgct atcgtcgatc gccgaactgc
     7501 gacatgccgg actcgcccag catgaagggc tcaaccgggc gggcttctcg gtcgcgcggc
     7561 ccgcgccccg aggcacgcgg cccatgcatc gcatccaccg cgtctcgccg tgctccgact
     7621 acctgggttg gcacgtcgac gccgccggcg acatcttccc gtgcatcggc atggtcgggc
     7681 atgcgccggc aaggctcggc aatatcgcgg gacggttcgt cgacgcgctg catgcgctgg
     7741 cggggacgcg ggacgcgatg gtcgggttgg ccgagcgagg ccccgatttc ctcgcgccgc
     7801 cggcctccgt gccgttcgac ctctgcgcgc tgcatcggga tgcggtgacg gacctggaat
     7861 ccatctgacg tgccgtcgaa acgaaccagg tggcttctgg gcagcatggg catgctgctc
     7921 gtggtgatag cgacatgcgc gatcgtcggc aaggcggcgg cgcccaggag ccgcgtgacg
     7981 acggcgcatc cgcgtgtccg cgacatcgaa cgacgcatcg aggcggccgg gacgatgcga
     8041 cccgtctcgg tcgtgcacgt gggggcactc gtgtccggag tcgtggtcaa tgcgacggtc
     8101 gaagccggtg atcacgtgca gcgcggacaa cggattgcca ccatcgacca ggaaccctac
     8161 cggctgcagc tcgagcgcgc tcatgccctc gcgcgcgcgg ctcagcggcg cgccgcgcag
     8221 gcgcgcagtg ccttgtaccg ccagcaggat cttcagcgcg cgggcttcat cgcgccagcc
     8281 gccttggatg cgtccgaggc ggcggcgcga gaagccgacg aggatgtccg cgtcgccttc
     8341 gccgacgtcg agacggcgcg catcaacctc gcccattgcg aaatcgattc gcccatctca
     8401 ggcatcgtgt tgaccaagga aatcgcggca gggcagtcgg tcgcttcatc gttccaggtg
     8461 ccagacctgt tcaccatcgt gtcgcgcttg gacaggctgg aggtcgtcgc gatgttcgcc
     8521 gaggcggacc tcggcgccgt gacgccgcat gccgaagtca agctgcaggt gccggccttc
     8581 cccgaccgga atttcacggc gaccgtcgaa cgcgtgctgg acacgccgga aagccggcag
     8641 ggcgtcgtga tgttccctgt ggtgctcggc ctcgccaacg ccggcgggct gttgcgcccc
     8701 ggcatgaccg cctatgcgga gctgcgggtc gccagcgtcc gaggcgcgtt gaccgttccg
     8761 cacaaggcgg tcgccttcgc tcgttctcgt gatcgcgccg cgcaccagga ggcctccggg
     8821 ccgcggcatc tgtacgtcct gcgcaacggg gccgtcacgc cgatcaccgt ccagttcggc
     8881 gcttccgacg aactcgggac gcaggtgctg ccggtcgatc gtctcgatcc gcacgacgat
     8941 gtcgtgctga ctgattctcc gtgagtgaac catgtacctc atacccatgt cgccggctga
     9001 tatcccggcc taccttcgtg gctttgccga ctggcacgcg caagcgctgc tcgacgtcgc
     9061 gatcggtgac ggccgggaca tcgacgaatt gcacgcgcga gcgcaggcgc agatacagcc
     9121 ccagttcggg gagaacggcc tgccggtcgg atcgacgatc ttcgacctct gcacgaacga
     9181 tgcgaacgaa agggtcggag tcctctgggc gggtggcgcg gacttcggct tcgggccgct
     9241 gttctatatc cacgatcttc gaatccatgc gccgttccga cgtcgtggct tcgctcgcga
     9301 agccctggac gaggtgtacc agatcgcgaa ggagcgtggc ggcgttgtcg gcgtcgcgtt
     9361 gagcgtgttg gccggcaatg ccgcggcgcg cgaactctat cgagcgagca gcttcagggt
     9421 gctgtccgaa gtcatggtga gggccctttg agtcatcgca gcacctcggg cgtcgccacg
     9481 ccaccgttcg gccgaggtcg ccgaacggcc actttcgctc cgtgattccg gcctcaatga
     9541 aaagcgcaat caccctcatc cgctccgtga gcgtcgcgct gctggcgtac gtcctcatcg
     9601 atggcccatt gggaatgctc ttgcagagcg cgctgcccgg tgaaaccgat ggacggggac
     9661 attcggtggg actgccgctg atgctgggcg acctgacgat ccatggcatc gcactcttcg
     9721 cggcgggtgc tctggccgcc agcacgacat ccgagtcgtc gccgcgaggg gccttcgtgg
     9781 ccagcgcggc ggttttcgcg ttcgtgctcg gactcaccct cgcgttgtgg gatgccgagc
     9841 cggcatggtt cgacatcgcc tgcctcctca tgacgccgcc attccagttg ctggcatctg
     9901 cctggcatcg cgccgcagga aaggatccgc acgcatgagc aatcgtatgt cccccgacga
     9961 ccccgaccgc ggcattgcca cgatgatgtc cttctcttcc tcgcaatcga gttcgcctcc
    10021 catcgaatgt tcgcgtgcgc tgccgtcgcc cgcagggcgt ggcccgtcga cgtggcagct
    10081 gctgcgcgcc gagagcggcc gccagcgcac gcttctggcg cgctatcccc tcgaggcggc
    10141 ggtgacggtc gtcggcttcg cctgcatgtt cctgatcttc gcggcgatcg gcacggtgtc
    10201 gaccggtccc tacgcgatct tcagcggcag cctgcagtcg ctggcgctga tctacgtcct
    10261 ctggacgatg tccgcggcga cggtggtcgc gggcgcctcc caggtcagcg ccgacgccgc
    10321 gatcggcgtg ctggagaacc tgttcctctc gtgctcgccg atcatccgca tcctgtcggc
    10381 gcgcacggcg gcgcggatct gcctgagcgt gccgatgggg ctcgtcctcc tggccgcctt
    10441 ctgcctcggg acagcttggg cgcctcctcc agtcgtggtg gcagcgatgg ccgtggcagc
    10501 gcttgcctgc gggctcaccg gactcgggct cggcttcgca ttcgcgggcg ccgcgctggt
    10561 caccaagcgg atcgtggtgc tgggcatgcc cgtgaacttc ctgtgcatgc tggccttcat
    10621 gtcgcagcat gcggcggcgg gcgatcggct cgacaaccca gtgctgtact tgcccttcgt
    10681 cgccgccgcg accttggtgc gccgcgcggt ggagctggga cgcttcgagc ccgtgctggt
    10741 ggcccaggct ctggtcggcg tcctggtctg ggtgccgctg ggtcgcctcg tgctggcgcg
    10801 ctgcgtccaa gcatgccggc gcgtgggatc ggtcaatctg tactgagcat gtcgacgtcc
    10861 gagatcttcg cgatcgatgc cgcgggcctg tcgcgcatct tccatccgcc cggcgcgtcg
    10921 cgggtcgagg ccttgccgcc acgcgacttg cgcgtacggg cgggcgagag cgtggcgctc
    10981 accggcgcat ccggttgcgg caagagcacg ctcctgggcc tgctgtcgct gatggaggcg
    11041 cccagcgcgg gcgtactccg actcttcggc accgacgttt cggtgctggg gcgctcggca
    11101 cgggcacgtt tccgccagca gcacatcggc gtcgtgttcc agaacttctg cctgctgaag
    11161 gatcgcagcg tcctggcgaa cgtcgaactg ccgctggcct acgccgagct ctcgccggcg
    11221 aagcgccgcg agcgggcgct cgattggctg gggcgcgtcg gactcgggaa cctggcgcac
    11281 cgtcatccca ccgagttgtc cggcggccag cagcagcgcg tcgcgatcgc gcgcgccatg
    11341 gcgacggggc cgcgcctgct gctcgccgac gagccgaccg gcagcctcga ccgcgacacc
    11401 ggcgagcgtg tgagcgacga cctgctgcgt ttctgcgcgg acaccggcgc gacctgcgtc
    11461 atcgcgacgc acgaccccct gctcgccgca cgctgcgctc gcgtggaacg gatggatcgc
    11521 gatgcgtgcc gctgacttcg cccgagaggt gtccggggcg ctgctgcgcg accgcctgcg
    11581 ggcggcgctg acggtggccg ggatcgcggt cggctccttc gcggtggcgg cgctgctgag
    11641 cctcggggcc ggcgtccagg gcgcgatcgg cgactcgctg aaggggctgg gcgagaacct
    11701 cgtgatcgtg acgccggagc ggtcccggtc gggcgcgctc gctggcgcgg cggcgcaggc
    11761 ggcggcattg acggaggccg acgtgcggga acttcgccgg ctcgacgcgg tgcaggccgt
    11821 gtccggcgtc gtgaccgcgc gggtgtcggc ggccgcgggg ccggagtcga tcaccaccac
    11881 ctgcctcggc gtcgatggcg aatacgccga cgtcgtccgg ctcgagctgg acgagggcgg
    11941 cgctgcgcgc gacgagctcg gcgcgtcgcc ctggccggtg gccgtgctgg gtgcgtccgt
    12001 ggccgcgcgg ctttttccgg atggcgatgg ggtcgggcgg cgcatcgagc tttccgggca
    12061 ccgcgtgcag gtcgtgggcg tcgcgcggga acgcggccgc gacgccgggg gcgcggacga
    12121 ggacgacttc gtgctcgtgc cccgggcctt cgcacgcgag cagctcgtcg cgcatccgac
    12181 gcccgatggc atcgacgcgg tgttcgcgct tgccgtcccg ggcgaggtgg acgacgcggt
    12241 ggaggaggcc tcggaggcgc tgggccgccg gcgtccgggc gtggagctga aggacgccct
    12301 cgccgtgaat tcgatgcgcg gactgatgtc ggccagcgtg gccgcggcgc ggctgctgac
    12361 cgtgctcatg ggcggcatgg cggcgatctc tgtgctggtg ggcggcgtgg gtatcgtcaa
    12421 cgtgatgctc gccaacgtgg ccgatcgacg cctggagatc gggctcaagc tcgcattggg
    12481 ggcgccgccg gtcgtcatcg gcctcgaatt cctggccggc gccgtggcgt tcgcgctggc
    12541 gggcgcggtc gcgggactgc tgggcgcctg gatgctcctg cgcctgctgt cgatggccca
    12601 ggtcgcggac gtgtccctca cgcctgcggc caccgcgctc gccacggcgc tcgcgggcat
    12661 cgtcggcgtc gtggccggcg cctatccggc cgtccaggcg gcccgtcttc aacctcggga
    12721 gatattgcgt gatgcttgac gaacccacgg cactggcgct ggccgacgtc gacaaggcgt
    12781 tcgcggcgcg cggtcgccgc cgacaggtcc tggcgggggt gcacctgcgg gcggcggccg
    12841 gcgagctggt cgcgatcacc ggtccgaacg gcgccggcaa gtccacggtg ctgcggctcg
    12901 tcgccggact gctgctgccg gacgccggca cggtgcgcgt cgcggatcgg cgcggctgcc
    12961 tgtgcgatcc ggcgggccag cctggcctcg tgtcggcggt gttcgacggc agccgtggct
    13021 tgtactggaa gctgaccgtg gccgagaacc tgcgctattt cgcgagcctg gacggcctgt
    13081 ccggtcccgc ggcgatggcg gccgcggcgc cctggctgga acgcttcggc ctcgaggcgc
    13141 gacaggacga tctcgtgcag aacctgtcca agggaacgca gcagaaggtg tccatcgtgc
    13201 gcgcgctcgc gcttgcgaag ccggtgctgc tgttcgacga gccgacggta ctgctcgacg
    13261 aggatgcctg ctggcagctc gcgcaaagcc tgaagtcgct ctgccgccag ggccacacgg
    13321 tggtcgtcgc gactcacgac cgcgacttcg tcggtctgac gcaggcgcgc caggtgcgag
    13381 tcgtgcccga gggcgggctc gtggctgcgg cggtcgcgcc ggtgcgcacg gaggcggcaa
    13441 cgctgctgca ttgaggaccg gcgaggcacc cggcgttcgg ccagagccga cgattgccgg
    13501 caatcgtccc cgacgacgga tgtccagacc tcccgacagc gggctcccgc gtgatttagt
    13561 aggcgtccac cccgcgattc catgcttcgg ctcttcagcg acggtcgcat gatccgaaga
    13621 accgactatc cgcgcagggc tggctgcccg tcggcgccgt tcgtccagct tcttccaacg
    13681 ccgtccaacg gcgacacggg gtgccgcagt gcagttgctt ccgctccaag aagtcgcgag
    13741 ccagatcaag ccaggcagcc cgctggcatg gggcatccgc gattcgaacg gcaagctgct
    13801 gctggcgcgc ggacaccgcg tcgccgacgc cgcgatgctg gagaccctgc tcgcgcgcgg
    13861 catgttcgtc gacgccgacg agatgcgcgc cgcaggccgc tccggggcgc cgccgaagga
    13921 gggcttcttc ggtcgctggc gcttgtccag cgcgcggctg aataccttgc tgacgagccc
    13981 gcccgcccag ctgcgcgcgg cgatggacga ggtcgtgggc gtgttgatgg cgctggccga
    14041 gcgttatccc gacaaggtcc tgttccagat cctgcgccac gaccagacgc gcctgcagac
    14101 ctatggcgtc tcgcactcgt tgcactgcgc ggccgtgtgc tgcctgaccg gcaagcgcat
    14161 gggctgggac gaggcgcaac ggcgcacgct cgtgagcgcc gcgatgtcga tgaacctgtc
    14221 catgatcgaa ctgcagggcc gattggccgt gcagggcgcc aggcccacgc cggagcagcg
    14281 cgccgcgatc gaggcgcatc cgcacaagag cgccgagatg ttgcgtgcga gcggcgtcga
    14341 cgacgcgctg tggctggccg cggtggaaca gcatcacgag ctggtcgacg gctcgggcta
    14401 cccgctgcgc cggaccgacg tcgccgagtt cgcgcaggcg ctgcactacg tggacgtgtt
    14461 caccgcgaag ctcagcgccc gttccacccg cggcccgctg ccggccaacg tcgcggcgcg
    14521 cgagatcttc accgcgagcg gcggccaccc gctcgccgcg gcgctgatca aggaattcgg
    14581 catctatccg ccgggctgct tcgtgcgcct ggcttccggc gagaccgcga tcgtcctgca
    14641 ccgcggcgcg accgtgaaca cgcccatcgt cgcctgcctc accaagccca acggccagcc
    14701 gatgatgcgt cccgtgcagc gcgagacgac atcgagggac gccgcagtcg tcgccatcgt
    14761 gcccgacggc gacgtgatgg tgcgcgtgcc ttgggagacg ctctactcgg acgactgagc
    14821 tcgtcgcagg ccctcgcggc cacgccccgg gagcgcgctg aaaaggcgag ggctcgggcc
    14881 ctggcgattc aacgccggcc tttggtcgtt ggcgtcgcgc gagctactcg ggatgccgct
    14941 ggccccagtc gacggcgttg gcttcgtcca gggcggccat gccctcgtcc caggccgcgg
    15001 ccatgccctc gagcagcgtg ccgacctcgg tcgcccgcgg cgacgagacg gcgcgggcga
    15061 gcttgaggat cagcgacttg gcgtcgcggg cgagcccgtc gaaggcctcg ccggcgcgtt
    15121 gcagcgcagc gtcgccctcg gagccgggca gcgcctgcca ggccaggccg cgggcggcca
    15181 ggtcgaaggc ggcgccaagc tggcgcacgg tgccgaaggc ccagcgatgg tagtagtcga
    15241 ggccgtggtc gcgcagcagc gggaggtcgt cgaccaggcg tcgcgcgaag cggcgtaccg
    15301 ggttgtcgcg cggacggaag gcgaggtggt cggccaggtg gcgctgggcc agcagccgca
    15361 gctcgctcgt cgagcgcgcg atgaggcgat cggtgcggat gaactcggcg aacagcggca
    15421 gccggccctc gtcgagcgcc agcgtcgccg ccccgatcgt cgccacgcgc gtgccacgcc
    15481 agtcggcgat ggcctcgagg tcggagagcg cgtcgggcag gccgagcagg tgttggcagt
    15541 cgtcgcccga ggcctcgaaa tagccggcgt tgtggaagta gcggaggcgg cgagcggcga
    15601 ggtcgacctc gttgaggacg atggtggtct tcgcgtgctg ccggtggtag tcggtgcccg
    15661 cggtgtccgg cagccagaag gcgtccacct ccacgctcag cagccggccc gcggccaggt
    15721 gctcgatcgc gtgctcggcg agcgggcgcc agaccgtgag ctcctggacg tcgatgccgt
    15781 acagcgcgcg cagttcggcg tgcggcggct tgaagaacgt ccactggtcg ccctcgaagt
    15841 cgatcgccgt gacgcagggc cacagcgcgt gcgggtcgag ctccagcgcg gacagcagct
    15901 cgatccagag atcgacgtag cagttcttct ccagccaggt cgcgtcgggc ccgtgcagcg
    15961 cgtgcggtgt ccagcccgca gcgctcaggc cg
//
LOCUS       DASVLN010000333         5764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_447785, whole genome shotgun sequence.
ACCESSION   DASVLN010000333 DASVLN010000000
VERSION     DASVLN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447785"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VF453_08175"
     CDS             complement(<1..763)
                     /locus_tag="VF453_08175"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX7687668.1"
                     /translation="MKAGRRWLQGSLGARISVLFLALLLAVQLASFAALRASLTAHAQ
                     RVLPQRLLEGERLLQTLLDRKAQSVTAGAQLLAADYGFREAIATNDADTIASVLANHG
                     ARIGATEAALLAPDFRLRAATTAHSREFAQLASRLAAQAARAGHADTIALLDGRPYQA
                     VLVPVKAPLLAGWVVMAFPLDAQLVADMRNLSGLDLTLLARASARGPWAAVLSSLDAD
                     RVGSLCARPWQGASAASDAMRTVSSQGEVLGVRAIP"
     gene            complement(760..1377)
                     /locus_tag="VF453_08180"
     CDS             complement(760..1377)
                     /locus_tag="VF453_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HEX7687669.1"
                     /translation="MPRRFAISASAAVLAAATLSARAAAVSVVVTDSAGGPLVDAVVS
                     LEPAGAHVAPKPLQGVQVAQHDLQFDPQVTVIPVGTPVLFPNNDTVRHHVYSFSPAKT
                     FQIKLYAGVPHAPIVFDKPGVAVLGCNIHDQMVAWVVVVDTPYYARTGAGGRLRIDGV
                     PPGSYQMKVWHSSLPEDAPPQAQALAVGAADVEQRVRLAPGAVAR"
     gene            complement(1532..3394)
                     /locus_tag="VF453_08185"
     CDS             complement(1532..3394)
                     /locus_tag="VF453_08185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7687670.1"
                     /translation="MSTAEIDRLALTALRLLPDEPERAMRIWADAFARAAEAGPDAGL
                     ERPLLRLHVVQLNHEARYGDRDALGPRIVAAAEMAQEHGWVVEELLINLLLLFLGTLG
                     TDHDEGLRDIQAIRELAEAHLEPIELSWLDLMAGHFRGYLHGWAEEQKRAYRALNRLF
                     ECPDAPAGLIANTKKNIGYSHLLVHNLDLARQYLEEAWRAYEPLPLTPRKVSACKAWA
                     QCLVTLGDYRRADHVMAPVLAARHGVATPLYMASALLVAAEARIALGDAASASALLAE
                     AQGLANASVEPAIHVHAAYVRALLLHERDDIAGAAAAGEAGCGLITTHTHEIAVHSCL
                     ALTARLQALAGSFRRAYELQVRLMDLRSELSNKAAEIRHIDLHVDHQTHLARMEIEHA
                     RRERTLAEAAEAAIVQKNLLLEQRLREVERLQESLRDLADRDALTGLFNRRYLAGALP
                     GLLARKATQGSRVTLVLIDLDHFKAVNDRHGHQMGDRVLCGFAELLRETFRADALCCR
                     YGGEEFCVLMTDTDDASARALVADLLERLAAITFAVGDRTLTGVGFSAGVASFEADAG
                     VDMDLVFRRADQALYAAKNAGRRRISTFQEQTAAPAPTPGRTAGQATDAPRERA"
     gene            complement(3580..4218)
                     /locus_tag="VF453_08190"
     CDS             complement(3580..4218)
                     /locus_tag="VF453_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687671.1"
                     /translation="MVNAGRRQLQLAALAFAAAAAGGCAAPGSAPVQHSTYTETISSV
                     LVSEDNAHIAAIGASHHYIFDAPPLLVRALHSPAHGSLQATFSTFHVARDGKVTGEYE
                     LALPAGAPADVQKAAADIGLQHEPDGRWDVHGMLSGQRYTGWTYKLGREQDRLNQPYT
                     IEFTTDFTAADRAVDKAATPIRTAADGVQLIYYAPLAPVILPIIFLSKARDH"
     gene            complement(4353..5051)
                     /gene="kdpE"
                     /locus_tag="VF453_08195"
     CDS             complement(4353..5051)
                     /gene="kdpE"
                     /locus_tag="VF453_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HEX7687672.1"
                     /translation="MSPHDSPGKVLVVEDEAEIRRFIRLALQAESLEVAEAQSVQRGL
                     VEAGTRRPDLVVLDLGLPDGDGLDFIRGLRTWSEIPIIVLSARTREADKIAALDAGAD
                     DYLVKPFGTGELLARVRAQLRRHPRASAEAQSVLAFGDVRIDLVRRTVERAGAPVHLT
                     PIEYRLLTHLAAHPNRVLTHPQLLRAVWGPSHAEDTHYVRIYMGHLRAKLEADASQPQ
                     HLLTELGVGYRFVP"
     gene            complement(5048..>5764)
                     /locus_tag="VF453_08200"
     CDS             complement(5048..>5764)
                     /locus_tag="VF453_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7687673.1"
                     /translation="GLADALRLTAPPLAPPHAEIAASLREAAVRMNALVNNLLDMARL
                     ESGLESGRLALDLQWQPLEEVVGSALRGAGALAAGGHPVRVQLPPGLPLLRFDAVLVE
                     RVLANLLENAAKYTPAGSVVEIGAAVAGEAVEVWVADTGPGLPPGREALIFEKFERGR
                     RESATPGVGLGLAICRAIVGAHGGAIRGENRPGGGARFVFTLPRGEPPALAEDEGDPA
                     AAPGPTDTGDPSPPAHGVHE"
BASE COUNT          733 a         2075 c         2202 g          754 t
ORIGIN      
        1 gggggatcgc gcgcacgccc agcacctcgc cctgcgagga cacggtgcgc atcgcgtcgg
       61 aggcggcgga cgcgccctgc cagggccgcg cgcacaggct gcccacgcgg tcggcgtcca
      121 ggctggacag cacggccgcc cacggcccgc gggcgctcgc gcgcgcgagc agcgtgaggt
      181 cgaggcccga gagattgcgc atgtcggcga cgagctgcgc atcgagcggg aacgccatca
      241 cgacccagcc cgcgagcagc ggcgccttca ccggcaccag gacggcctgg taggggcggc
      301 cgtcgagcag cgcgatcgtg tccgcatggc ccgcccgcgc ggcctgggcg gccaggcggc
      361 tcgcgagctg cgcgaactcg cgcgagtggg cggtggtggc ggcgcgcagg cggaagtcgg
      421 gcgcgagcag cgccgcctcg gtcgcgccga tgcgtgcgcc gtggttggcg agcaccgagg
      481 cgatggtgtc ggcgtcgttg gtcgcgatcg cctcgcgaaa gccgtagtcg gccgcgagca
      541 gctgcgcgcc ggcagtcacg gactgcgcct tgcgatcgag cagcgtctgc agcaggcgct
      601 cgccctcgag caggcgctgc ggcagcacgc gctgggcgtg tgcggtcagg ctcgctcgca
      661 gggccgcgaa gctcgccagc tggacggcca gcagcagcgc gaggaacagc acgctgatgc
      721 gcgcgcccag cgagccctgc agccagcgcc ggccggcctt cacctggcga ccgctcccgg
      781 agccagcctg acgcgctgct ccacgtcggc cgcgccgacg gcgagcgcct gcgcctgcgg
      841 cggggcgtcc tcgggcaggc tcgagtgcca caccttcatc tggtagctgc cgggcggcac
      901 accgtcgatg cgcagccggc cgccggcgcc cgtgcgcgcg tagtacggcg tgtcgacgac
      961 gaccacccag gccaccatct ggtcgtggat gttgcagccg agcacggcca cgccgggctt
     1021 gtcgaacacg atgggggcgt gggggacgcc ggcgtacagc ttgatctgga aggtcttggc
     1081 cggcgagaac gagtagacgt ggtgccgcac cgtgtcgttg ttcgggaaca gcaccggcgt
     1141 gccgacgggg atcaccgtga cctgcggatc gaactgcagg tcgtgctgcg cgacctgcac
     1201 gccttgcagc ggcttgggcg cgacgtgggc gcctgccggc tccagcgaca ccacggcgtc
     1261 gaccagcggg ccgcccgcgg agtcggtgac gacgaccgag acggccgccg cccgcgcgga
     1321 cagggtcgcg gcggcaagca cggcggcgga ggcggagatc gcgaatcggc gaggcatcgg
     1381 gcgggcggaa gcgggcagca cggcacccgg cgaccggggg acgccgggtc caggactggc
     1441 aggatgcgct atctatcggc catcttgccc gcgctcttga ccgggctggg cacgcggccg
     1501 agggcgggag ccgcctcccg cgcgcattcc gtcacgcgcg ctcgcgcggc gcgtcagtcg
     1561 cctgcccggc cgtgcgcccg ggcgtgggcg cgggcgcggc ggtctgctcc tggaacgtcg
     1621 agatccggcg ccggccggcg ttcttggccg cgtagagcgc ctggtcggcg cggcggaaga
     1681 cgagatccat gtcgacgccc gcatccgcct cgaaactcgc gacgccggcc gagaagccga
     1741 cgcccgtgag cgtgcggtcg ccgaccgcga aggtgatcgc ggccagtcgc tcgagcaggt
     1801 ccgccaccag cgcgcgtgcc gacgcgtcgt cggtgtcggt catcagcacg cagaactcct
     1861 cgccgccgta gcggcagcac agcgcatcgg cgcggaaggt ctcgcgcagg agctcggcga
     1921 agccgcacag cacgcggtcg cccatctggt ggccatggcg gtcgttgacg gccttgaagt
     1981 ggtcgaggtc gatcagcacc agcgtcacgc gcgagccctg cgtcgccttg cgcgcgagca
     2041 gccccggcag cgcgccggcg aggtagcggc ggttgaacag gcccgtgagc gcatcgcggt
     2101 cggcgaggtc gcgcagcgac tcctgcagcc gctcgacctc gcgcaggcgc tgctcgagca
     2161 gcaggttctt ctgcacgatc gccgcctccg cggcctcggc gagggtgcgc tcgcggcggg
     2221 catgctcgat ctccatgcgc gcgaggtgcg tctggtggtc gacgtgcagg tcgatgtggc
     2281 ggatctccgc ggccttgttg gacaactcgc tgcgcaggtc catcaggcgc acctggagct
     2341 cgtacgcgcg ccggaagctg cccgccaggg cctgcagccg cgccgtcaac gcgaggcagc
     2401 tgtggaccgc gatctcgtgc gtgtgggtgg tgatgagccc gcagccggcc tcgcccgcgg
     2461 cggcggcgcc ggcgatgtcg tcgcgctcgt gcagcagcag cgcgcgcacg taggcggcgt
     2521 ggacgtggat cgcgggctcg acggacgcgt tcgccaaccc ctgggcctcg gccagcagcg
     2581 cggacgccga cgcggcgtcg cccagcgcga tccgtgcctc ggcggcgacc agcagcgcgc
     2641 tggccatgta cagcggcgtc gcgacgccgt ggcgggccgc gagcaccggc gccatcacgt
     2701 ggtcggcgcg ccgatagtct cccagcgtca cgaggcattg cgcccacgcc ttgcaggccg
     2761 agaccttgcg cggcgtcagc ggcagcggct cgtaggcgcg ccaggcctcc tcgaggtact
     2821 ggcgcgcgag gtcgaggttg tgcaccagca ggtggctgta gccgatgttc ttcttggtgt
     2881 tggcgatcag gccggcgggg gcgtcggggc actcgaacag ccggttcagc gcccggtatg
     2941 cacgcttctg ctcctccgcc cagccgtgca ggtagccgcg gaagtggccg gccatgaggt
     3001 cgagccagct cagctcgatc ggctccaggt gcgcctcggc cagctcgcgg atcgcctgga
     3061 tgtcgcgcag gccctcgtca tggtcggtgc ccagggtgcc gaggaacagc agcagcaggt
     3121 tgatcagcag ctcctccacg acccagccgt gctcctgcgc catctcggcc gcggcgacga
     3181 tgcgcgggcc cagtgcgtcg cggtcgccgt agcgcgcttc gtggttcagc tgcacgacgt
     3241 gcaggcgcag caacgggcgc tccagcccgg cgtccggccc cgcctcggcg gcccgggcga
     3301 acgcgtcggc ccagatccgc atcgcgcgct cgggctcgtc ggggagcagg cgcagcgcgg
     3361 tcagcgccag cctgtcgatt tcggcggtcg acatcgggac ggtcaacttt ctcggggcgc
     3421 cggcgagccg gcgccgggtg gcgcacgtcc cggcaacctc cggccggtcg ggcgcatctt
     3481 gggcgaaccg tgcgttatac gccttcgcgg tcggccgcgc atcccccacg gtcgccggcc
     3541 gtccgcccct gccgcagcgc cgccgattcg ttcacgcgtt cagtggtcgc gcgccttgct
     3601 caggaagatg atgggcagga tcaccggcgc cagcggtgcg tagtagatca gctgcacgcc
     3661 gtcggcggcg gtgcggatcg gcgtggccgc cttgtcgacc gcccggtcgg cggcggtgaa
     3721 gtcggtcgtg aactcgatcg tgtagggctg gttgagcctg tcctgctcgc ggcccagctt
     3781 gtaggtccag ccggtgtagc gctggccgct cagcatgccg tggacgtccc agcggccgtc
     3841 gggctcgtgc tgcaggccga tgtccgcggc ggccttctgg acgtccgcgg gcgcgccggc
     3901 gggcagcgcg agctcgtact cgcccgtcac cttgccgtcg cgcgcgacgt ggaaggtcga
     3961 gaacgtcgcc tgcagcgagc cgtgcgccgg cgagtgcagc gcccgcacca gcagcggcgg
     4021 cgcgtcgaag atgtagtggt ggctcgcgcc gatcgccgcg atgtgcgcgt tgtcctcgga
     4081 cacgagcacc gacgagatcg tctcggtgta ggtcgaatgc tgcaccggcg cgctgcccgg
     4141 cgccgcgcat ccgcccgcgg ccgccgccgc gaaggccagc gccgccagct ggagttgacg
     4201 tcgacccgca ttcaccatga gctgctccgc ctgcggccgc ccgaggcggc gccgttgtcc
     4261 gtggaatggc gcggattatc ggcggcggcg cggggcggcg tctgccgcgg attggcgttt
     4321 aggggcgcgc gccgagaccg gcgccgcacc ggtcacggca cgaaccggta gccgaccccc
     4381 agctccgtca gcaggtgctg cggctgcgac gcgtcggcct cgagcttggc gcgcaggtgg
     4441 cccatgtaga tgcggacgta gtgcgtatcc tccgcgtgcg acgggcccca gacggcgcgc
     4501 agcagctgcg ggtgggtgag cacgcggttc gggtgcgcgg cgaggtgggt gagcagccgg
     4561 tactcgatcg gcgtcaggtg cacgggcgca ccggcgcgtt cgacggtgcg gcgcacgagg
     4621 tcgatgcgca cgtcgccgaa cgcgagcacg ctctgcgctt cggcgctcgc gcgcggatgg
     4681 cggcgcagct gcgcccgcac gcgcgcgagc agctcgccgg tgccgaaggg cttgacgagg
     4741 tagtcgtcgg cgcccgcgtc gagcgcggcg atcttgtcgg cctccctcgt gcgcgcggac
     4801 agcacgatga tcgggatctc cgaccacgtg cgcagcccgc ggatgaagtc gaggccgtcg
     4861 ccgtcgggca ggccgaggtc gagcaccacg aggtcgggcc gccgcgtgcc ggcctccacc
     4921 agcccgcgct gcacggactg ggcctcggcg acctcgagcg actcggcctg cagcgcgagg
     4981 cggatgaagc gccggatctc ggcctcgtcc tcgacgacga gcaccttgcc gggcgagtcg
     5041 tgggggctca ttcgtggact ccgtgggcgg gcggggatgg atcgccagta tcggtcggcc
     5101 cgggcgcggc ggcgggatcg ccctcgtcct cggccagcgc gggcggctcg ccgcgcggca
     5161 gcgtgaagac gaagcgcgcg ccgccgccgg gccggttctc gccgcggatg gcgccaccgt
     5221 gcgcgccgac gatcgcgcgg cagatcgcca gcccgaggcc gacgccgggc gtcgcgctct
     5281 cgcgccggcc gcgctcgaat ttctcgaaga tcagcgcctc gcgaccgggc ggcagccccg
     5341 ggcccgtgtc ggcgacccag acctcgaccg cctcgcccgc gaccgccgcg ccgatctcga
     5401 ccacgctacc cgccggcgtg tacttggcgg cgttctccag caggttggcg agcacgcgct
     5461 cgaccagcac cgcgtcgaag cgcagcaacg gcagcccggg cggcagctgc acccgcacag
     5521 gatgcccgcc cgccgcgagc gcgcccgcgc cgcgcagcgc gctgccgacg acctcctcga
     5581 gcggctgcca ctgcaggtcg agcgccaacc ggcccgactc gaggccggac tccaggcgcg
     5641 ccatgtccag caggttgttc acgagcgcgt tcatgcgcac cgcggcctcg cgcagcgacg
     5701 cggcgatctc cgcatgcggc ggcgccagcg gcggcgcggt caggcgcagc gcgtccgcga
     5761 ggcc
//
LOCUS       DASVLN010000334         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_392602, whole genome shotgun sequence.
ACCESSION   DASVLN010000334 DASVLN010000000
VERSION     DASVLN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392602"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF453_08205"
     CDS             complement(<1..213)
                     /locus_tag="VF453_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687674.1"
                     /translation="MKARSWHGASVIGSYTGLGIWPGTSRSGAGRRCGNGRARIRREL
                     ALLEARLDVPVPRATIWTTLLPTTAPP"
     gene            415..1254
                     /locus_tag="VF453_08210"
     CDS             415..1254
                     /locus_tag="VF453_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7687675.1"
                     /translation="MTDTPTSPRWSMSDLDLSRADPARVRGDDALFLLVCSASFIESG
                     SDTYTRNLVERFADDPEVAGWLSRQWEHEELQHGRALKAYVQHVWPGFDWESAYADFF
                     AEYSRICTMEELEPTRGQEMAARCIVEMGTTTYYTALHDACDEPVLRELFWRIRTDEV
                     QHYKHFYGYFQKYREIDRLHRGQVLTALVRRLLELRREDATIALRHAVAWRLRTGAAD
                     GGPAPIADADELTRQAMALVSRHLPMELAVRMTIKPLLLQPGLQRAMVRPLAALGRGV
                     LLH"
     gene            1323..>1936
                     /locus_tag="VF453_08215"
     CDS             1323..>1936
                     /locus_tag="VF453_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7687676.1"
                     /translation="MTPDADDLLLFARVAQAGSFSRAAERVQLPKSTVSRRIAALEKR
                     LGERLLQRTTRKLVLTDFGRGVLDHARVLSEEVDAALAFALSRQARPSGRLRVSMPGD
                     FASVALESALAGFARDYPDVQLEIDLSPRRVDLIGENFDLAIRMGELQGDAQLAARRL
                     AVFGSGLYAAPGLLREHGVPLAPEALESMPGLMLLSRGGDALPWS"
BASE COUNT          257 a          729 c          677 g          273 t
ORIGIN      
        1 cggcggcgcc gtcgtcggca gcagcgtcgt ccagatcgtc gcgcgcggca ccggcacgtc
       61 gagccgtgct tccagcagcg ccagctcgcg ccggatgcgt gcccggccgt tgccgcatcg
      121 tcgacccgcg cccgaccgtg aagttccggg ccatataccc agccccgtat atgaaccgat
      181 gacggacgct ccgtgccacg acctggcctt cacgcccgcg tcgcggcgtc gggcagcccg
      241 cccgtgacgc cagatgacgc gccccgggcg cgccgcactt ccttgcgcca gcctgtcgtg
      301 gaaccgaacg cgcgtcgacg gacaccaatg tgccgcggat ccggtgcgat gctgcggctc
      361 ctggtcgcga cgaggcgcac cgcgctccgc acgccgattg ccgcaagcac ttccatgacc
      421 gacaccccga cttctccgcg ctggtcgatg agcgacctgg acctgtcgcg cgccgacccg
      481 gcgcgggtgc gcggcgacga cgcgctgttc ctgctcgtct gcagcgcctc tttcatcgag
      541 agcggcagcg acacctacac gcgcaacctc gtcgagcgct tcgcggacga cccggaggtc
      601 gcgggctggc tgagccggca gtgggagcac gaggagctgc agcacggccg cgcgctcaag
      661 gcctacgtgc agcatgtgtg gcccggcttc gactgggaat ccgcctacgc ggacttcttc
      721 gccgagtatt cgcgcatctg cacgatggag gagctcgagc cgacgcgcgg ccaggagatg
      781 gcggcgcgct gcatcgtcga gatgggcacg acgacgtact acaccgcgct gcacgacgcc
      841 tgcgacgaac cggtgctgcg tgaactgttc tggcgcatcc gcaccgacga ggtccagcac
      901 tacaagcact tctacggcta cttccagaag taccgcgaga tcgatcgcct gcaccgcggc
      961 caggtgctca cggcgctcgt gcgccggctg ctggagctgc gccgcgagga cgcgacgatc
     1021 gcgctgcgcc acgcggtcgc ctggcgcctg cgcaccggcg ctgccgacgg cgggccggcg
     1081 ccgatcgccg atgcggacga gctcacgcgc caggcgatgg cgctcgtcag ccgccacctg
     1141 ccgatggaac tggccgtgcg catgacgatc aagccgctgc tgctgcaacc cggcctgcag
     1201 cgcgcgatgg tgcggccgct ggcggcactc gggcgcggcg tgctgctcca ctgacgcggg
     1261 cactccggcc ggcgctggca tgatgcgcgg atcgcccgat ccgccgctcg ccccgtgccg
     1321 ccatgacgcc cgacgccgac gacctcctgc tgttcgcgcg cgtcgcgcag gccggcagct
     1381 tcagccgcgc ggccgagcgc gtccagctgc cgaagtccac cgtgtcacgc cgcatcgccg
     1441 cgctcgagaa gcggctcggc gaacgcctgc tgcagcgcac gacgcgcaag ctcgtgctga
     1501 ccgacttcgg ccgcggcgtg ctcgatcacg cgcgcgtgct gtcggaggag gtggacgccg
     1561 cgctcgcgtt cgcgctttcg cgccaggcgc ggcccagcgg ccggctgcgg gtgtcgatgc
     1621 cgggcgactt cgcgagcgtc gcgctcgaat cggcgctggc cggcttcgca cgcgactatc
     1681 ccgacgtgca gctcgagatc gacctctcgc cgcgccgggt cgacctgatc ggcgagaact
     1741 tcgacctggc gatccgcatg ggcgagctcc agggcgacgc ccaactcgcg gcgcgccggc
     1801 tcgcggtctt cggcagcggc ctctacgcgg cgcccgggct gctgcgcgag cacggcgtgc
     1861 cgctggcgcc cgaggcgctg gagtccatgc cggggctgat gctgctctcg cgcggcggcg
     1921 acgcgctgcc gtggtc
//
LOCUS       DASVLN010000335        11090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_125363, whole genome shotgun sequence.
ACCESSION   DASVLN010000335 DASVLN010000000
VERSION     DASVLN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11090
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125363"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..894
                     /locus_tag="VF453_08220"
     CDS             106..894
                     /locus_tag="VF453_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7687677.1"
                     /translation="MIEMDDLHKRFESGRGRARRTVLAVDGLSMRAADGAITGLLGPN
                     GAGKTTSLRMLVGLVTPDRGTMRVDGIDLRTDPQRVRARVGVLSDARGLYPRLTAREN
                     IVYHGRLHGMGADEAHARCDHLGRMLELRDLLDRRTDGFSQGERMKTALARALVHDPP
                     NIVLDEPTNGLDVIATRALRETLRSLRDASGKCIVFSTHIMQEVQRLCDRVVVVSHGR
                     HVAEGTVEELCEQAGCHDFEDAFVRLAFGAGEVVGAGRVEEGRR"
     gene            891..2066
                     /locus_tag="VF453_08225"
     CDS             891..2066
                     /locus_tag="VF453_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7687678.1"
                     /translation="MSAWRVTMLVFAKELRDALRDRRTLLLVLLSSVALGPLMLVALS
                     GLLGQLEKQAEAREIDVVGLERAPTLANYLARQTLVARTPPPDWEAQLRDNRFSEPVV
                     IVPPDFEVALAHGEAPVLQLVFSSANTGSQGGARRVQAALQGFDQEQGRLRLIARGVA
                     PGVMNAVQVDAHDIADIAARAAQFTGMLPFLVMMAVVYGALNAAQDTTAGERERGSLE
                     PLLATPAGRTALAVGKWAAVSCVGVMIAVLSCLSFIPAQHLLRSDSFAALMRFGWGEA
                     AWFIALLIPLAAAAAALMMAIAVRCRTVKEAQATNAILITLLTLSSISQLMGGQHGES
                     AWQLWLPAAAQLTLMDRVLKGMPIGVGDVAPMVAVCAAVTVAAVGYVSRQLASRAAQ"
     gene            2232..3017
                     /locus_tag="VF453_08230"
     CDS             2232..3017
                     /locus_tag="VF453_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEX7687679.1"
                     /translation="MRTSFPWSPAALSSDREGNMFRPRMLSGRHLRVWEAQTVEDFKK
                     EVVGFARSLEFNTVSTVYVIDQSATHTEFFAIDNVPVGYHQTFHDVNFGRRDPVMQHC
                     KANSIPLVWDWRNYRAAGEMDIWEEQAAFGIRQGIALALHMPLGRHFTIGLDWNQSPT
                     HSAGRLTDMIAEAQLFAVHANEAARRIFDPECLPGRQIELTPRELEALRWTVDGKSAR
                     EISDILNISERTVVFHLHNATVKLKCRNKFHACIKAMQLRILS"
     gene            3031..3759
                     /locus_tag="VF453_08235"
     CDS             3031..3759
                     /locus_tag="VF453_08235"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="HEX7687680.1"
                     /translation="MIFDLHKKCSMLYEQKTVSELFKFTECLTHELDFATFSITVIVD
                     HSPYLTQFQNITNAPSSYLDHYNDPAECAIDPASQHCKTSSAPIVWNQSTYVEHGVGW
                     LWERQAQFGYESGLQRAIHLPHGRHLMFGVDCDRPLISSPRSAKAIVNDFMVLTPYIQ
                     AVAFELSDIGARPDGYSELSGLEIDILRWSMGGHNADWIAKRARLSEQTIHLKISNIL
                     KKLDCSTRYEAVVKAIRKGFIVSD"
     gene            complement(3760..3954)
                     /locus_tag="VF453_08240"
     CDS             complement(3760..3954)
                     /locus_tag="VF453_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687681.1"
                     /translation="MAWDWIEVAEGIVAMVDPMRMVTNMQLITLEGKILAATEAALHF
                     NQFVRRLPWQEEVGRLLKTY"
     gene            complement(5608..5898)
                     /locus_tag="VF453_08245"
     CDS             complement(5608..5898)
                     /locus_tag="VF453_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7687682.1"
                     /translation="MAPPSSAASPTTLACRPGCGACCIAPSITSPIPGMPHGKPAGVP
                     CVQLDAEFRCRLFGHPDRPAFCGGLQPAADMCGNSRTEALHWLATLEVATRP"
     gene            6074..8110
                     /gene="mnmG"
                     /locus_tag="VF453_08250"
     CDS             6074..8110
                     /gene="mnmG"
                     /locus_tag="VF453_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX7687683.1"
                     /translation="MNYPQEFDVIVVGGGHAGTEAALAAARMGCATLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAATDEAGIQFRILNGSKGPAVRATRAQADRI
                     LYKAAIRRRLENQPNLWLFQQAVDDLVVESRGGRDRVSGAVTQAGITFRGKTVVLTAG
                     TFLDGKIHVGLQNYSAGRAGDPPAQRLSARLKELQLPQRRLKTGTPPRLDGRSIDFSK
                     LTEQPGDGMPDSDGRVTTPMPVFSFMGSAAQHPRQMPCWVTHTNARTHEIIRSGFDRS
                     PMFTGVIEGVGPRYCPSIEDKINRFADKDAHQIFLEPEGLTTHEFYPNGISTSLPFDI
                     QLAAVRSMPGLENAHILRPGYAIEYDYFDPRGLKNSFETRAIDGLFFAGQINGTTGYE
                     EAAAQGLHAGVNAALRARGGDATFVLRRDQAYLGVLVDDLNTKGVTEPYRMFTSRAEF
                     RLQLREDNADLRLTELGRNLGLVDDARWVHFERKREALAREAERLRSTWVHPGILPAL
                     HAERLVGKALEREYALVDLLRRPGVDFDSIAEVAHIARPDADVSRETLEARLGEQLAG
                     EVIEQLEIATKYAGYIGKQNEEVTRAQELENLPLPADLDYGQVTALSFEVRQKLNQHR
                     PQTLGQATRISGVTPAAISLLLVHLKKKRFKGFATPSTTDAVDAVSTDERRNAA"
     gene            8202..8780
                     /gene="rsmG"
                     /locus_tag="VF453_08255"
     CDS             8202..8780
                     /gene="rsmG"
                     /locus_tag="VF453_08255"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX7687684.1"
                     /translation="MSYLGLIQRWTKVYNLTAVRDPAEMFTHHLLDCLAVVAPMRAGT
                     GAAGQGALRVLDVGSGAGLPGVVVALLNPAWQVTCIDAVAKKASFIRQVAAELGLPNL
                     HGVHGRVEDKTNFRTPEFDLVTSRAFASLKDFVELTRPLLASAGQWAAMKANLSDEER
                     AGLPRNVDMFHVEQLDVPELGAQRCLVWMKPQ"
     gene            8879..9691
                     /locus_tag="VF453_08260"
     CDS             8879..9691
                     /locus_tag="VF453_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7687685.1"
                     /translation="MSRIFCIANQKGGVGKTTTTVNLAAGLAQIGQRVLLVDLDPQGN
                     ATMGSGIDKRGLPTSVYDVLLESAGVAEAVQRSESGGYDVLGANRELAGAEVELVPLE
                     RRNERLRGALKAVDAAYDFVLIDCPPSLSLLTLNGLCSAHGVIVPMQCEYFALEGVSD
                     LVNTIKQVHANLNRDLELIGLLRVMFDPRITLQAHVSEQLKAHFGDKVFNTVIPRNVR
                     LAEAPSYGQPGVVFDPTSRGATAFLDFAREMVARVQAPSLAAAPRTSTAESR"
     gene            complement(9725..10474)
                     /locus_tag="VF453_08265"
     CDS             complement(9725..10474)
                     /locus_tag="VF453_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7687686.1"
                     /translation="MSTTAEMINVLKAELKLARLTYADLGRELGLAESSVKRMFAKGD
                     MPLKRIDEICRVLKCDFAELARKVADLELLRDELSVEQERAVVADPRLLVAALCALSQ
                     WPYEQIVSTYDFSEAEVVRHLVHLDRLGIIELKPLNRYRLKVAKTFRWRPQGPVMQFF
                     RERGLSDYFAGGFDGENEMFGLVHGHLSGPMAKVFQERLQRLCQDFAQQHLTDQRLPR
                     THKRGYTAIVSLRAWTFGPLRELLRDPSAEP"
     gene            10552..11043
                     /locus_tag="VF453_08270"
     CDS             10552..11043
                     /locus_tag="VF453_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687687.1"
                     /translation="MSGVAVLRIIANTSATRCTMKPVSLRSRRVPALGAAFAIACLAQ
                     FEPARAQSSDRLSEASGVSVAVSVALPVMFVAGAGSVVVTAVQATGDGIVWVVENVAD
                     GARASVRVTGHAVGASAVAVGTVLTVTATSAGLLLSAAGQVVAFIPNEIGRALLYDRQ
                     VSQ"
BASE COUNT         1953 a         3641 c         3570 g         1926 t
ORIGIN      
        1 cccgcaggcg ccgtcccgcg cgtccgcggc cgtgccgtcg tcggccgcgt ccagcctcgc
       61 cggcgccgcc gccgagcccg cggacgaccg cctgaaggac gcccgatgat cgagatggac
      121 gacctgcaca agcgcttcga gtccgggcgc ggccgcgcca ggcgcacggt gctggcggtg
      181 gacggcctgt cgatgcgcgc cgccgacggc gcgatcaccg ggctgctcgg ccccaatggc
      241 gccggcaaga ccaccagcct gcgcatgctc gtcggcctcg tgacgcccga tcgcggcacg
      301 atgcgcgtcg acggcatcga cctgcgcacg gatccgcagc gcgtgcgggc gcgcgtcggc
      361 gtgctgtccg atgcgcgcgg cctctacccg cgcctgaccg cgcgcgagaa catcgtctac
      421 cacggccgcc tgcacggcat gggcgccgac gaggcgcatg cgcgctgcga ccacctcgga
      481 cggatgctgg agctgcgcga cctgctcgat cgccgcaccg acggcttcag ccagggcgag
      541 cgcatgaaga ccgcgctcgc gcgcgcgctg gtgcacgatc cgcccaacat cgtcctcgac
      601 gagcccacca acggcctgga cgtcatcgcc acgcgggcgc tgcgcgagac gctgcgctcc
      661 ctgcgcgacg cgtccggcaa gtgcatcgtg ttctcgaccc acatcatgca ggaggtgcag
      721 cggctgtgcg atcgcgtggt ggtcgtctcg cacggccgcc acgtcgccga gggcacggtc
      781 gaggagctgt gcgagcaggc cggctgccac gacttcgagg acgccttcgt gcgcctcgcg
      841 ttcggcgccg gcgaggtggt gggcgcgggc cgagtcgagg agggcaggcg atgagcgcgt
      901 ggcgcgtgac gatgctcgtg ttcgcgaagg agctgcgcga tgcgctgcgc gatcgccgca
      961 cgctgctgct cgtgctgctg agctcggtgg cgctcgggcc gctcatgctg gtcgcgctgt
     1021 cgggactgct cgggcagctc gagaagcagg ccgaggcgcg cgagatcgac gtcgtggggc
     1081 tcgagcgggc gcccacgctg gccaactacc tcgcgcggca gacgctcgtc gcgcggacgc
     1141 caccgcccga ctgggaggcg cagctgcgcg acaaccggtt ctccgagccg gtggtgatcg
     1201 tgccgccgga cttcgaggtg gcactcgcac acggcgaggc ccccgtgctg cagctcgtct
     1261 tcagcagcgc gaacaccggc tcgcagggcg gcgcacgccg cgtccaggca gcgctgcagg
     1321 gattcgacca ggagcagggg cggctgaggc tcatcgcacg cggcgtggcg cccggggtca
     1381 tgaatgccgt ccaggtcgat gcccacgaca tcgccgacat cgccgctcgc gccgcccagt
     1441 tcaccggcat gctgccgttc ctcgtcatga tggccgtcgt ctacggcgcg ctcaacgccg
     1501 cgcaggacac caccgccggc gagcgcgagc gcggctcgct cgagccgctg ctcgccacgc
     1561 ccgccggccg cacggcgctg gccgtcggca agtgggcggc ggtgtcctgc gtcggcgtga
     1621 tgatcgccgt gctgtcgtgc ctcagcttca tcccggcgca gcacctgctg cgcagcgact
     1681 ccttcgccgc gctcatgcgc ttcggctggg gcgaggccgc ctggttcatc gcgctgctga
     1741 tcccgctcgc cgccgccgcc gccgcgctga tgatggccat cgccgtccgc tgcagaacgg
     1801 tgaaggaagc gcaggcgacg aacgccatcc tgatcacgct gctcacgctg agctcgatct
     1861 cgcagctcat gggcggccag cacggggagt ccgcctggca actgtggctt ccggcggcgg
     1921 ctcagctcac gctgatggat cgcgtgctca agggcatgcc gatcggcgtg ggcgacgtag
     1981 cgccgatggt ggccgtctgc gcggcagtga ccgtcgccgc agtgggatac gtcagtcgcc
     2041 aactggccag ccgggctgcg cagtgagccg cggccgtccg agactccgac ccgcacgcgc
     2101 cgcccctttc agccgaacca gcgacggtcg agcggatttc cccaccgggg aaactcatcg
     2161 gcatgctgcc ggcgaaccgg tgaaacatcc cgccaattgc gcaagaaagc cataggagct
     2221 tcctcgtcgc agtgagaaca tcgttcccgt ggtcaccagc cgcgctgtcc agcgatcggg
     2281 aaggaaatat gttcaggccg agaatgctca gcggacgcca tctgcgcgtg tgggaggcgc
     2341 agacggtcga ggatttcaag aaggaagtcg taggatttgc tcgctcatta gaatttaata
     2401 ccgtttcaac ggtatacgtt atcgatcaat ccgcgactca taccgagttt tttgcaattg
     2461 acaatgtccc cgttggatat catcaaacct ttcatgacgt caatttcggg cgtcgcgacc
     2521 ctgtgatgca gcactgcaag gccaattcta ttcccctagt ttgggattgg cgaaactacc
     2581 gcgccgctgg tgaaatggac atatgggagg agcaggccgc atttgggata aggcagggga
     2641 tagcactggc gctacacatg ccgctcggtc ggcactttac gatcgggctg gattggaacc
     2701 aatctcccac tcattcagcg ggccgcctca cggacatgat tgcagaggca caactgtttg
     2761 cagtccatgc caacgaagcc gcgcgcagaa tttttgatcc cgagtgtttg ccgggcagac
     2821 agattgaact aaccccccgc gagctcgagg cgcttcgatg gacggtcgat ggtaaatcag
     2881 cccgcgaaat tagcgatatt ttgaacatat cggagcgaac cgtcgtattt cacttgcaca
     2941 acgcaacggt caagctgaag tgtcgaaaca aatttcatgc ttgcataaag gctatgcagc
     3001 taagaatact ctcctgattt gggagaagca atgatcttcg acctccacaa gaagtgctcg
     3061 atgctctatg agcaaaaaac cgtctcggag cttttcaaat tcacagaatg cctcacgcat
     3121 gaattggatt tcgctacgtt ttccatcacc gtgatcgtcg accattcgcc ttatttgaca
     3181 cagtttcaaa acatcaccaa tgcgccgagc agttatctcg atcactacaa cgatccagcc
     3241 gaatgcgcga ttgacccggc tagccagcac tgcaagacgt cttccgctcc tatagtctgg
     3301 aatcaaagca cctatgtcga acacggtgtt ggatggcttt gggagcgtca agcgcaattt
     3361 ggttatgaga gtggtctgca gcgcgccata catctacccc atgggcgaca tttgatgttt
     3421 ggggtggatt gcgatcgccc attaatttcc agcccaaggt cggccaaggc aatcgttaat
     3481 gatttcatgg ttcttacgcc atatatacaa gccgtagcgt tcgagctttc cgatataggc
     3541 gctcggcccg atggatactc cgaactcagc ggtcttgaga tagatatctt gcgttggtcg
     3601 atgggcggtc ataacgccga ctggattgca aaaagggccc gcctgtcgga acaaactata
     3661 cacttgaaaa tttctaatat cttgaaaaag cttgactgca gcacgcggta cgaggcagta
     3721 gttaaggcta ttcgcaaagg ctttatagtg agtgactaat taataggttt ttaatagtcg
     3781 acccacctct tcctgccaag gaagtcgacg aacaaactga ttgaaatgca aagccgcctc
     3841 tgtggcagcc aagatcttgc cttccagggt aattagctgc atgtttgtta ccatgcgcat
     3901 aggatcaacc atcgccacga tcccctcggc gacttctatc caatcccaag ccaagccagc
     3961 gtctccgtcg cccgacaggc gcccccaaat ggtgttacct cgacgcggtc cgtttcgatt
     4021 cggagtccgt gtaacccgcg tggccagatg catgaactgc ggacgccgac gcaagttggt
     4081 tcgcaaatat actgtcggcc aagagtctag cgcccagggg ggcggagcaa acgatgcggc
     4141 cgagctcgat gggcattgat tgacggtttg catgccctca gtctggggaa aacacttcct
     4201 ccggactagc tgtcaacttt gtcaggtcgg gtgctcgaac gactaaagtg cagtcttctt
     4261 cagaggcagg tctgatctca cgacctgcct ctgccaagcc ctcacgcccc caatgcgact
     4321 tctatgccac cggaggtaga cctgtgagct ctcaaatgct acagggcggc tttcttgccg
     4381 tgatgcaagc gaagacaccc cttgactttc gccgtgagct tgttcggttc ggggagtcgc
     4441 ttggctttaa gaccatcgcc gccaccacga tcgtagatcg acagagcggc cctgaatttc
     4501 actctgttga caacactccg cttgcctact cgcaatcatt cagagatccg agcttaggca
     4561 aggcagaccc ggtcatgcag cactgtaagc gagcggcagt acccatcatc tgggaccaag
     4621 acacgtatgt ctcgcgtggg aaggcggacg tttgggacga tcaggcccga tacggctacc
     4681 gtacaggcat cgcgctggct ctgcatctcc ccgaaggtaa gcacttcgtt ttgggagtgg
     4741 atagagaccg cccgttgcct cgatcacctc gaacgataac gagaattgtt gcggacctgc
     4801 agttgttcgc agtgcatgca caagacgcgg cattccgaat atttgcacct tcctctgaac
     4861 agttggagcc gcctcctcta acagcgaggg agcttgaggc gctcaggtgg actatggacg
     4921 gtaagaccgc gtgggaggta ggcgcgatca tgggcatttc cgaaaggacc gcggtactgc
     4981 acctccaaaa tgccacgcat aagctgaact gcgtgaccaa gcaccaagcg gtgttgaagg
     5041 ccgtccgact tggcatcgtc ggctgatccg gataagccat cctgtaagac ttgacagtgt
     5101 ctagctgaga tcggaactgc ttcaatcact cccatggcgc cgagactttg gcgcggttac
     5161 cggagattga acgatgaagc gcgctctcgt cagcatgatc accgcggctc gacaagcgtg
     5221 tcgccagcgt gccgacgaca tcctcatcgt agacgttcac atgccggtcc gcacactatc
     5281 gattggactc ctttcgaaag tttgcggcgc aggttcgggc gcagacacgc ctaaaggcac
     5341 ctggagcgcc tctatcgtta ccgtctaacg ttcgcagcaa gaaccctcca caatcagcca
     5401 ccaactccat cgcgtcaccc agtgccgcga ccgcgcagtt gacgctgatt gactcttggg
     5461 gtgccgacgg cgcccgccgt cggcaccccg ctcttttttg agggttcgcc gattccccag
     5521 gactccgctc gccgcgccgg cttcacgtga agcagcgcgg cgaagtcgtt tttggggggc
     5581 gggtcgaggg gttcgtggac agctgggtca gggacgggtg gcgacctcga gggtcgcgag
     5641 ccagtggagt gcctccgtgc gggagttgcc gcacatgtcc gccgcaggct gcaggccgcc
     5701 gcagaaggcc ggccgatcgg gatggccgaa gaggcgacag cggaattcgg cgtcgagctg
     5761 cacgcatggg acgccggcgg gcttgccgtg cggcatgccg gggatcgggc tggtgatcga
     5821 cggggcgatg cagcatgcgc cgcagccggg gcggcaggcg agggtagtcg ggctcgcggc
     5881 ggacgacggt ggggccatgc cggattgtcc gcgttctcca cgtcgatggc gcgcgtgcgc
     5941 cgggccgctt aaaatcgcgg cttccccaaa cccgtctccg tgcggcgccc gtgaggtgcg
     6001 accggcgcgt cgtagtgtgg gcttttcctt cgatccggcc gcgacggtac ggatccagcg
     6061 cagtgagttg ccggtgaact acccccagga attcgacgtc atcgtggtcg gcggcggcca
     6121 tgccggcacg gaagcggcgc tcgccgcggc ccgcatgggc tgcgcgacgc tgctgctgac
     6181 ccacaacatc gagaccttgg gccagatgag ctgcaatccg tcgatcggcg gcatcgggaa
     6241 aggtcatctg gtcaaggagg tcgacgccct gggcggcgca atggcggccg cgaccgacga
     6301 ggcgggtatc cagttccgca tcctcaacgg ctcgaagggc ccggccgtcc gtgcgacgcg
     6361 ggcccaggcc gaccgcatcc tctacaaggc ggcgatccgc cgccggctcg agaaccagcc
     6421 caacctgtgg ctgttccagc aggccgtcga cgatctggtc gtcgagtccc gggggggtcg
     6481 ggaccgcgtc agcggcgctg tcacgcaggc gggcatcacc ttccgcggca agaccgtcgt
     6541 gctgacggcg ggcaccttcc tggacggcaa gatccacgtc gggctccaga actacagcgc
     6601 cgggcgcgcg ggcgatccgc cggcccagcg gctgtcggcg cgcctgaagg agctgcagct
     6661 gccgcagcgc cgcctcaaga ccggcacccc gccgcgcctc gatggccgct ccatcgactt
     6721 ctccaagctc accgagcagc ccggcgacgg catgccggat agcgatggcc gcgtcacgac
     6781 gccgatgccc gtgttcagct tcatgggctc ggcggcgcag catccgcggc agatgccgtg
     6841 ctgggtcacc cacaccaacg ctcgcacgca cgagatcatc cgttccggct ttgaccgcag
     6901 tccgatgttc accggggtca tcgagggcgt cggcccgcgc tactgcccca gcatcgagga
     6961 caagatcaat cgcttcgcgg acaaggatgc gcaccagatc ttcctcgagc cggaaggctt
     7021 gaccacgcac gagttctacc cgaacgggat ctccacgtcg ctgcccttcg acatccagtt
     7081 ggcggccgtg cgctccatgc ccggactcga gaacgcgcac atcctgcgac cgggctatgc
     7141 gatcgagtac gactatttcg atccgcgcgg cctgaagaat tcgttcgaga cccgggcgat
     7201 cgacgggctc ttcttcgccg ggcagatcaa cggcacgacc ggctacgagg aggccgctgc
     7261 gcagggactt cacgccggcg tgaacgcagc gctgcgcgcg cgtggcggcg acgcgacctt
     7321 cgtgctgcgg cgcgatcagg cctacctcgg tgtcctggtc gacgacctga acaccaaggg
     7381 cgtgaccgag ccgtaccgca tgttcacgag ccgggcggaa ttccggttgc agctgcgcga
     7441 ggacaacgcc gatctgcgcc tgaccgagct cggtcgcaac ctcggcctgg tggacgatgc
     7501 gcgttgggtg cacttcgagc gcaagcgcga agcgctggcc cgggaggcgg agcggctgcg
     7561 ctcgacctgg gtgcatccgg gcatcctgcc tgcactccac gctgagcgat tggtgggcaa
     7621 ggcgctcgag cgcgagtacg cgcttgtcga cctgctgcgt cgtccggggg tggatttcga
     7681 ctccatcgca gaggtcgcgc acatcgctcg tcccgacgct gatgtttcac gtgaaacact
     7741 ggaggcccga ctcggtgagc agctcgctgg cgaggtgatc gagcaactgg agatcgccac
     7801 caaatacgca ggctacatcg gcaagcagaa cgaggaggtt acccgcgcgc aggagctcga
     7861 gaacctgccg ctgccggccg atctcgacta cggccaggtg acagcgctct cgttcgaggt
     7921 tcgtcaaaag ctcaatcaac accggccaca aacgttgggc caggcaaccc gcatctcggg
     7981 tgtgacaccg gcggccatct cgctgctgct ggtgcacctg aagaagaagc ggttcaaggg
     8041 attcgcgacc ccgtccacga ccgacgccgt cgacgcagtc agcaccgatg agcggcgcaa
     8101 tgcggcctga cgctgcctcg ccggccgcac aagcggatcg tctcgccgag ctcgccccgc
     8161 gacttggact cgagctcagt ccgacagact gcgaacgctt gttgagctac ctcggcctga
     8221 tccagcgctg gacgaaggtc tacaacctca ccgcggtgcg tgatcccgcc gagatgttca
     8281 cgcaccacct gttggactgc ctggccgtcg tcgcgcccat gcgggccggc acgggcgcgg
     8341 cagggcaggg cgctctgcgc gtgctggacg tcgggagcgg cgccgggctg ccgggcgtgg
     8401 tcgtggcgct gctgaatcct gcctggcaag tcacctgcat cgacgcggtg gccaagaagg
     8461 caagcttcat ccgccaggtc gccgcggagc tcggtctgcc caatctgcac ggtgtccacg
     8521 ggcgcgtcga agacaagacg aatttccgga cgcccgagtt cgatctcgtg acctcgcgcg
     8581 ccttcgcgtc cctgaaggac ttcgtcgagc tgacgcgacc gctgttggcg tcggcggggc
     8641 agtgggcggc catgaaggcg aacctgtccg acgaggagcg cgcgggattg cctcggaacg
     8701 tcgatatgtt tcacgtggaa caactggacg tgcccgaact gggcgcccag cgctgcctcg
     8761 tgtggatgaa accgcaataa agcgtcaatc gcgggctgtc cccgatcgac gccagccccg
     8821 acaataggcg gcatcgtccg acgccctccg cccgaccttc cgagctccac ggcccttcat
     8881 gtcgagaatc ttctgcatcg cgaaccagaa aggtggcgtc ggcaagacga cgaccaccgt
     8941 caacctcgcg gccggtctcg cgcagatcgg ccagcgcgtg ctgctggtcg atctcgatcc
     9001 ccagggcaac gcgacgatgg gctcgggcat cgacaagcgc ggcctgccca ccagcgtcta
     9061 cgacgtcctg ctggagagcg ccggggtcgc cgaggccgtc cagcgctcgg agtccggggg
     9121 ctacgacgtg ctcggcgcga atcgtgaact cgcgggcgcc gaggtcgagc tggtaccgct
     9181 ggagcgccgc aacgaacgct tgcgcggtgc cctgaaggcg gtcgacgccg catacgactt
     9241 cgtgctcatc gactgcccgc cgtcgctgag cctgctgacg ctgaacggcc tttgcagcgc
     9301 gcatggcgtg atcgtgccga tgcagtgcga gtacttcgcg ctcgagggcg tgtccgacct
     9361 ggtcaacacg atcaagcagg tgcacgccaa cctgaaccgc gacctcgagc tgattggtct
     9421 gctgcgcgtc atgttcgacc cgcgcatcac gctgcaggcg cacgtcagcg agcagctgaa
     9481 ggcgcacttc ggcgacaagg tgttcaacac ggtgatccct cgcaacgtcc gcctcgccga
     9541 ggcgccgagc tacgggcagc cgggtgtcgt cttcgacccg acctcccgcg gcgcgacggc
     9601 gttcctcgat ttcgcccgcg agatggtcgc gcgcgtccag gcaccgtcgc tcgcggccgc
     9661 cccgcgcacc tcgaccgcgg aaagccgctg agcgcacgcg gcgcaaggca cggctcgggc
     9721 tggttcaggg ttccgccgac ggatcgcgca acagctcgcg caagggcccg aacgtccacg
     9781 cacgcaggct gacgatcgct gtgtagcctc gcttgtgggt tcgtggcagg cgctggtcgg
     9841 taaggtgttg ctgggcgaag tcctggcaca ggcgctgcag ccgctcctga aacaccttgg
     9901 ccatcggccc gctgaggtgg ccgtggacca ggccgaacat ctcgttctcg ccgtcgaagc
     9961 cgccggcaaa atagtcggac aggccgcgct cgcggaagaa ctgcatgacc ggaccctgcg
    10021 ggcgccagcg gaacgtcttg gccaccttga ggcggtagcg gttcagcggc ttcagctcga
    10081 tgatgccgag ccggtcgagg tgcacgaggt gccgcacgac ctcggcctcg ctgaaatcgt
    10141 aggtcgagac gatctgctcg tacggccact ggctgagcgc gcacagcgct gcgaccagca
    10201 gccggggatc ggcgaccacc gcgcgctcct gctcgaccga cagctcgtcg cgcagcagct
    10261 ccaggtcggc gaccttgcgc gcgagctcgg cgaagtcgca cttgagcacg cggcagatct
    10321 cgtcgatgcg cttgagcggc atgtcgccct tggcgaacat gcgcttgacg ctggactcgg
    10381 ccagccccag ctcgcgcccg aggtcggcgt aggtcagccg ggccagcttg agctcggcct
    10441 tcaggacgtt gatcatctcg gcagtggtgc tcacgaatcg cctccctgga atgcttgagt
    10501 atcccgacac aataccgcaa gaccaggaat cgagcacctg gcgcaaccac gttgtcgggc
    10561 gtcgccgtgc tgcgcatcat cgccaacacc tcggcaactc gttgcaccat gaaacccgtc
    10621 tcacttcgct cacgccgcgt gccggccctg ggcgccgcct tcgcgatcgc ctgccttgcc
    10681 cagttcgaac cagcgcgggc gcagtcgtcc gaccgcctca gcgaggcctc cggcgtgtcg
    10741 gtggccgtct ccgtggcact gccggtgatg ttcgtcgccg gcgcgggctc ggtcgtcgtc
    10801 accgcggtgc aggcgaccgg cgacggcatc gtctgggttg tcgagaacgt cgccgacggc
    10861 gcgcgcgcca gcgtgcgcgt gaccggccac gcggtcggcg cctccgcggt cgcggtcggc
    10921 accgtcctca cggtgacggc gacctccgcc ggcttgctgc tcagtgcggc cggccaggtg
    10981 gtcgccttca tcccgaacga gatcggccgc gcgctgctct acgaccgcca ggtgtcgcaa
    11041 tgagccgcgg ccatgttgcc tggagccgcc gcgtgcgcgc gatcctcgcc
//
LOCUS       DASVLN010000336         6442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_231709, whole genome shotgun sequence.
ACCESSION   DASVLN010000336 DASVLN010000000
VERSION     DASVLN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231709"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..715)
                     /locus_tag="VF453_08275"
     CDS             complement(50..715)
                     /locus_tag="VF453_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX7687688.1"
                     /translation="MRSSTRREFALQLTGLAGAAALAGLGLPRTARAQGTLIEGTDYL
                     RLSKPQSTPAPGKIDVIEFFWYGCPHCNALEPALEAWMAKLPHDVNFRRQPVAFDALK
                     QIHAQIFFTWEALGLVEQMHAKTFARFHVQRKPINREEDMLAFAKDSGLDVAKVKAAW
                     ESFGVQTRVHQAERMEQDYGIDHMPQFAVQGRWTTASSGPGRPEVLAVTDGLIALARK
                     GAA"
     gene            complement(799..1455)
                     /locus_tag="VF453_08280"
     CDS             complement(799..1455)
                     /locus_tag="VF453_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEX7687689.1"
                     /translation="MQRRAFSLRLTGLAGAAALASLGLPRGALAQGGPVAGRDYTKLD
                     NPAPTTTRPGHVEVVEFFWYACPHCFAFEPTLEPWAAKLPADVNFHRVPVAFDALKEV
                     HQQIYYTWEALGLVDQMHAKTFARFHVQHKPINREEDMLEFARESGLDVAKVKAAWES
                     FGVQTRMHQAKQLSDDYQVDGVPELGIAGRFTTSPSMGGPIAALATTDALVNVVRKGG
                     "
     gene            complement(1518..2234)
                     /locus_tag="VF453_08285"
     CDS             complement(1518..2234)
                     /locus_tag="VF453_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7687690.1"
                     /translation="MKPVLKNQKGGTALGMIIGLLLGLGVALVVALYVTHTPMPLINK
                     AQQRTPEQDAAEAERNKNWDPNAGLIGKPTTRPPAPVPTASAPAAPASDTAVVPRQPA
                     PALPTPADTPRVAPVPASAPPSSARSTRDAAAILNGQSPDGKPAPTSTATGGEVDAFI
                     YFVQVGAYSKPEDAEQQRAKLALAGIRATVTEREQNGRVVHRVRLGPFDRREDADAQL
                     DRAKSVDPEASIVRVDRTKQ"
     gene            complement(2240..3970)
                     /gene="argS"
                     /locus_tag="VF453_08290"
     CDS             complement(2240..3970)
                     /gene="argS"
                     /locus_tag="VF453_08290"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7687691.1"
                     /translation="MIQAKSELAQALAVAVAQVAPEAAVTPAFESPKQAAHGDLAVTV
                     AMQLAKPLKLAPRVVAERLVAALNEQPAVQRWVSALEIAGPGFINLRLAPAAKQAVVA
                     EVLDSGDRFGVQPANGKKVIVEFVSANPTGPLHIGHASQGALGDTLCNLLESQGWAVH
                     REFYYNDAGVQIGTLARSTQLRIQGFKPGDDCWPTDPDDPASKGFYNGDYIADIARDF
                     LAKATVKADDREFTASGDPEDLDSIRQFAVAYLRHEQDLDLQAFGVRFDQYFLETSLY
                     ASHAVDRTVAKLLDSGKTYEADNALWLRTTDYGDDKDRVMRKSDGTYTYFVPDIAYHL
                     DKWERGFVKAINVQGSDHHGTVARVRAGLQAAGEGVPQGWPDYLLHKMITVMRGGQEV
                     KISKRAGSYVTLRDLIDWTSRDAVRFFLVSRKADSEFVFDIDLALKQNDENPVFYVQY
                     AHARICSVIRQFTDKGGDLGAIAGADLSLLAAPAESALMLKLAEFPSVLTTAARDLAP
                     HDVAFYARGVAAAFHTYYAAERFLVDDAALAHARLALLSATRQVLRNALAILGVSAPE
                     AMSRDENPES"
     gene            4203..5573
                     /locus_tag="VF453_08295"
     CDS             4203..5573
                     /locus_tag="VF453_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7687692.1"
                     /translation="MSNPAEFQAEATVREAPHEAWPSSIGKYRIQGRLGDGATSVVFL
                     GFDDFAKREVAIKQLRLTSDTAPVDAHFWSRFFAAEAALVGRLNHPNVVQIYDAVSDD
                     RVPYLVMEYVPGVTLREFCRTDRLLPLEQIVEIGFKCAMALGYVYRQGLIHRDVKPAN
                     ILAVVQDGEVVDVKLSDFGSVLNTNSEHTQIHRVGSLAYMSPEQIDGNALDCRTDIYA
                     LAAVLYHLIAGRPPFDAQTQVALMNQIFHHEPPSLAELREGVTPALEAVILGALAKSP
                     GARPQDWEQFAQALSGLIVNHQVPRGRLQKVLDSERFNLLRSLEFFADFDDVELWEVV
                     HRAKWQRLHYGHRIYRRGQEGRTFHIIAQGEVEVFRDGQRVARLGAGTSVGEMAYLAP
                     SPDLRRHSADIIVSEPCTTISFTPESLMQLSAESRMRFDKAFIQVLVRRLHAAHETLA
                     HPRRVL"
     gene            5822..6136
                     /locus_tag="VF453_08300"
     CDS             5822..6136
                     /locus_tag="VF453_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEX7687693.1"
                     /translation="MKSVLTAIALTLAAAGAFAADAGAPAASKALTPQQQKMADCSAA
                     NKGKKGDEYKAAQKACLSGGAAPASAPMTQQQKMAACAKQNKGKKGDDYKAAQKACLS
                     AP"
BASE COUNT          879 a         2249 c         2334 g          980 t
ORIGIN      
        1 aggccggcga agccgcggcg cgtcgtcggc gcgcgcatcc gcgctgccct caggccgcgc
       61 ccttgcgggc cagcgcgatc aggccgtcgg tcaccgcgag cacctcggga cggcccggac
      121 ccgacgacgc cgtcgtccag cggccctgca cggcgaactg cggcatgtgg tcgatgccgt
      181 agtcctgctc catgcgctcg gcctggtgga cgcgggtctg cacgccgaag ctttcccacg
      241 cggccttgac cttggcgacg tccagcccgc tgtccttcgc gaaggcgagc atgtcctcct
      301 cgcggttgat cggcttgcgc tggacgtgga agcgggcgaa ggtcttcgcg tgcatctgtt
      361 cgaccaggcc cagcgcctcc caggtgaaga agatctgggc gtggatctgc ttgagcgcgt
      421 cgaaggcgac cggctggcgg cggaagttga cgtcgtgcgg caacttcgcc atccaggcct
      481 ccagggccgg ctcgagcgcg ttgcagtgcg ggcagccgta ccagaagaac tcgatgacgt
      541 cgatcttgcc cggcgccggc gtggactgcg gcttggacag gcgcaggtag tccgtgccct
      601 cgatcagcgt accctgcgcg cgggccgtgc gcggcaggcc gaggccggcg agcgcggcgg
      661 cgccggccag gccggtcagt tggagggcga attcacggcg ggtcgaggag cgcatggcgt
      721 cggggcggcg gatcggccgc ggcagggtgg cggggtgtgg cagcgaaggc ggaggcgtcg
      781 gcgcagggcg gtcgtcgctc agccgccctt gcggacgacg ttgacgaggg cgtcggtggt
      841 cgcgagcgcg gcgatcgggc cgcccatgga cggggaggtc gtgaagcggc ccgcgatgcc
      901 cagctcgggc acgccgtcga cctggtagtc gtcggacagc tgcttggcct ggtgcatgcg
      961 cgtctggacg ccgaagctct cccaggcggc cttcaccttg gcgacgtcca ggccgctttc
     1021 gcgcgcgaac tcgagcatgt cctcctcgcg gttgatcggc ttgtgctgga cgtggaaacg
     1081 ggcgaaggtc ttcgcgtgca tctggtcgac caggcccagc gcctcccagg tgtagtagat
     1141 ctgctggtgg acttccttga gcgcgtcgaa ggcgacgggc acgcggtgga agttgacgtc
     1201 ggccggcagc ttggcggccc acggctccag cgtcggctcg aacgcgaagc aatgcgggca
     1261 ggcgtaccag aagaactcga ccacctcgac atggcccggc cgggtcgtgg tgggcgccgg
     1321 attgtccagc ttcgtgtagt cgcggccggc cacggggccg ccctgcgcga gcgcgccgcg
     1381 gggcaggccc agcgaggcga gcgccgcggc gccggccagg ccggtcagtc ggagggagaa
     1441 cgcgcgccgc tgcatcgctg gaatcctttg ctggagatcg tcggtcgggt gctgagaggc
     1501 cagcccggcc gcaaagttca ctgtttcgtg cgatcgacgc gcacgatgga cgcctcggga
     1561 tcgacggact tggcgcggtc gagctgcgcg tcggcgtcct ccctcctgtc aaacggccca
     1621 agccggacgc ggtggacaac acgcccgttc tgttcgcgct cggtcaccgt cgcgcggatg
     1681 cccgcgagcg ccagcttggc gcgctgctgt tcggcatcct cgggcttgga gtaggcgccc
     1741 acttgtacga aatagatgaa ggcatcgacc tcgccgccgg ttgccgtcga cgtgggcgcc
     1801 ggcttgccgt cgggcgactg gccgttgagg atggccgcgg cgtcgcgcgt gctgcgcgcg
     1861 ctggacggcg gcgccgaggc gggcaccggc gcgacgcggg gcgtgtcggc cggtgtgggc
     1921 agtgcgggcg cgggctggcg cgggacgacc gccgtgtcgg aggcgggcgc cgccggcgcg
     1981 gacgccgtgg gcaccggtgc cggcgggcgc gtcgtcggct tgccgatcag cccggcgttc
     2041 ggatcccagt tcttgttgcg ctcggcctcg gcggcatcct gctcgggcgt gcgctgctgg
     2101 gccttgttga tcagcggcat cggcgtgtgg gtgacgtaca gcgccaccac cagcgccacg
     2161 cccaggccca gcagcaggcc gatgatcatg cccagggcgg tgccgccctt ctggttcttc
     2221 aggacaggtt tcacgaggtt cagctttcgg ggttctcgtc gcgggacatg gcctccggcg
     2281 cgctcacgcc gaggatcgcc agcgcgttgc gcagcacctg gcgcgtcgcc gagagcaggg
     2341 cgaggcgggc gtgcgcgagg gcggcgtcgt cgaccaggaa gcgctcggcg gcgtaatagg
     2401 tgtggaacgc cgccgccacg ccgcgggcgt agaacgcgac gtcgtgcggc gcgaggtcgc
     2461 gcgcggcggt cgtgagcacg gacgggaact ccgccagctt cagcatcagc gcggattcgg
     2521 caggcgcggc gagcagcgag agatccgcgc cggcgatcgc gccgaggtcg ccacccttgt
     2581 cggtgaactg gcggatcacg gagcagatgc gcgcgtgcgc gtactgcacg tagaacaccg
     2641 ggttctcgtc gttctgcttc agcgcgaggt cgatgtcgaa cacgaactcg gaatccgcct
     2701 tgcggctgac gaggaagaag cgcacggcgt cgcggctggt ccagtcgatc aggtcgcgca
     2761 acgtcacgta gctgccggcg cgcttggaga tcttgacctc ctggccgccg cgcatgacgg
     2821 tgatcatctt gtgcagcagg tagtcgggcc agccctgggg cacgccctcg ccggcggcct
     2881 gcaggcccgc gcgcacgcgc gcgaccgtgc cgtggtggtc gctgccctgg acgttgatcg
     2941 ccttaacgaa gccgcgctcc cacttgtcga ggtggtaggc gatgtcgggc acgaagtagg
     3001 tgtacgtgcc gtcggacttg cgcatcacgc ggtccttgtc gtcgccgtag tcggtcgtgc
     3061 gcagccacag ggcgttgtcg gcctcgtagg tcttgcccga atcgagcagc ttggccaccg
     3121 tcctgtccac ggcgtggctc gcgtagaggc tcgtctcgag gaagtactgg tcgaagcgga
     3181 cgccgaaggc ctggaggtcg aggtcctgct cgtggcgcag gtaggcgacg gcgaactggc
     3241 ggatcgagtc gaggtcctcc gggtcgcccg aggcggtgaa ctcgcggtcg tccgccttga
     3301 ccgtggcctt ggcgaggaag tcgcgcgcga tgtcggcgat gtagtcgccg ttgtagaagc
     3361 ccttgctggc cgggtcgtcc gggtccgtgg gccagcagtc gtcgcccggc ttgaaaccct
     3421 ggatgcgcag ctgcgtgctc cgggccagcg tgccgatctg gacgccggcg tcgttgtagt
     3481 agaactcgcg gtgcaccgcc cagccctggc tctcgagcag gttgcacagc gtgtcgccca
     3541 gcgcgccctg gctggcgtgg ccgatgtgca gcgggccggt cggattcgcg gagacgaact
     3601 cgacgatcac cttcttgccg ttggccggct ggacgccgaa acggtcgccg ctgtccagca
     3661 cctccgccac gaccgcctgc ttggcggccg gcgcgaggcg caggttgatg aagcccggac
     3721 cggcgatctc cagggccgag acccagcgct gcaccgccgg ctgctcgttc agcgccgcca
     3781 ccaggcgttc cgcgacgacg cgcggcgcca gcttcagcgg cttggccagc tgcatggcga
     3841 cggtgacggc caggtcgcca tgggcggcct gcttgggcga ttcgaaggcg ggcgtgacgg
     3901 cggcttccgg cgcgacctgg gcgacggcga ccgccagggc ctgcgcgagc tcggacttgg
     3961 cttggatcat ccgccgattc tagatggacg ccccgcgccg cccgggcggc gcttcgggca
     4021 ggtttccggg gtcggcgagg cgccgcggcc gcggccggct ggccgcggcg cgggtccccg
     4081 gcgcggaaca acccggaaat tgccggtttc tttgcacgat ccgttccccg gcccgtcgcg
     4141 caggatacat actggccgat gcctgcccgc ggcgcgggcg ggtcggacgc cgccaacccg
     4201 ccatgtccaa tcccgccgag ttccaagccg aagccaccgt gcgcgaagcg ccgcacgagg
     4261 cctggccgtc ctcgatcggc aagtaccgca tccagggccg cctgggcgac ggcgcgacca
     4321 gcgtcgtctt cctcggcttc gacgacttcg ccaagcgcga ggtggcgatc aagcagctgc
     4381 gcctgacctc cgacaccgcg cccgtcgacg cccatttctg gtcgcgcttc ttcgctgcgg
     4441 aggcggcgct ggtcgggcgc ctcaaccatc cgaacgtcgt ccagatctac gacgccgtca
     4501 gcgacgaccg cgtgccctac ctcgtgatgg agtacgtgcc cggcgtcacg ctgcgcgagt
     4561 tctgccgcac cgaccgcctg ctgccgctcg agcagatcgt cgagatcggc ttcaagtgcg
     4621 cgatggcgct gggctacgtc taccgccagg gcctgatcca ccgcgacgtc aagcccgcca
     4681 acatcctggc cgtggtgcag gacggcgagg tcgtcgacgt caagctgagc gacttcggca
     4741 gcgtgctcaa cacgaactcc gagcacacgc agatccaccg cgtcggctcg ctcgcctaca
     4801 tgtcgcccga gcagatcgac ggcaacgcgc tcgactgccg gaccgacatc tacgcgctcg
     4861 ccgccgtgct ctaccacctg atcgccggcc ggccgccgtt cgacgcgcag acgcaggtcg
     4921 cgctgatgaa ccagatcttc catcacgagc cgccttcgct cgccgagctg cgcgaaggcg
     4981 tgacgccggc gctggaggcc gtgatcctgg gcgcgctcgc gaagtcgccc ggcgcgcgac
     5041 cccaggactg ggagcagttc gcgcaggcgc tgtcgggcct gatcgtcaac caccaggtgc
     5101 cgcgcgggcg gctgcagaag gtgctcgact ccgagcgctt caacctgctg cgctcgctcg
     5161 agttcttcgc cgacttcgac gacgtggagc tgtgggaggt cgtgcaccgc gccaagtggc
     5221 agcgcctgca ctacggccac cgcatctacc gccgcggcca ggaggggcgc accttccaca
     5281 tcatcgcgca gggcgaggtc gaggtcttcc gcgacggcca gcgcgtggcg cgcctgggcg
     5341 cgggcacgtc ggtgggggag atggcctacc tcgcgcccag ccccgacctg cgccggcaca
     5401 gcgcggacat catcgtctcc gagccgtgca cgacgatctc gttcacgccg gagtcgctga
     5461 tgcagctcag cgccgagtcg cgcatgcgct tcgacaaggc gttcatccag gtgctggtgc
     5521 gccgcctcca cgcggcccac gagaccctgg cgcatccacg acgcgtccta tgacgctgcg
     5581 tcccgcggtg cgcagcgtcc actcaaaccc gcgaaacctt cccttgcgcg gctgacgact
     5641 cctcacgccg ggcgtcggct cgccgctaca ggcggccggc ccgcgcgtga ccggcgtcgt
     5701 tgacggcgga tttcctgctt gtgacgcccg ggcggctttg cttcaatgca tccatgccgc
     5761 tcccccgcgg catgccccgc cacccgcggc cgacccggct cgccatcaca aggaacgccc
     5821 catgaaatcc gtcctcaccg ccatcgccct gaccctggcc gccgccggcg cgttcgccgc
     5881 cgacgccggc gcgcccgccg ccagcaaggc gctcacgccg cagcagcaga agatggccga
     5941 ctgctcggcg gccaacaagg gcaagaaggg cgacgaatac aaggcggccc agaaggcctg
     6001 cctgtcgggt ggcgcggccc ccgcgtccgc gccgatgacg cagcagcaga agatggccgc
     6061 ctgcgccaag cagaacaagg gcaagaaggg cgacgactac aaggccgcgc agaaggcctg
     6121 cctgtcggcg ccctgatttc ccgccggggc cccggctgcc gacggcgaaa cgaggggttt
     6181 ccggccgttt cgacgcgggc gtggcgggac gtggcatttc cggcccgttt cgcgcccggt
     6241 gtctcctccg accccggcga gcatggatcg tccgcggccg cgccttcgag ggcgcgccgc
     6301 gcggcgttcc atcaaccgct ctcgaaggag accccatgac ccatcgcatc ctgttcctgt
     6361 cgctggcgct cgtcgccggg ttcgcccagg cgcgcgcgcc gcacgcggcg gccgcgtccg
     6421 cgccggccgc ggtcgtcgcg tc
//
LOCUS       DASVLN010000337         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_179034, whole genome shotgun sequence.
ACCESSION   DASVLN010000337 DASVLN010000000
VERSION     DASVLN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179034"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VF453_08305"
     CDS             <1..804
                     /locus_tag="VF453_08305"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HEX7687694.1"
                     /translation="LGVVDGLRARFDVVQYGRLDYAPETFPLLAIRNCEWRDDLPCAL
                     VTGGVHGYETSGVHGALEWMARAFDRRAGALNLLVLPCISPWGYETINRWNPDALDPN
                     RQFRAGSPAAESALAMACVAAHGGRVDLHVDLHETTDTDNSEFGPAKAARDGTAFDWH
                     AIPDGFYLVGDSERPAPEFQRALIEAVRGVTHIAGADERGRLIGTPLAQPGVINYPKR
                     SLGLCGGMTAARFVTTTEVYPDSASTSPAECIAAQVATVEAAIEFLLRG"
     gene            886..4434
                     /gene="dnaE"
                     /locus_tag="VF453_08310"
     CDS             886..4434
                     /gene="dnaE"
                     /locus_tag="VF453_08310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7687695.1"
                     /translation="MAFVHLRTHTEFSVVDGTLRIDDVAAAAKADGQVALGITDLSNL
                     FGAVKFYKACRGKGVKPIVGVDVWMDPLPDAADRGATRLVLLVQDRQGYLNLCEMLGR
                     SWTRNVQRAQAWVRWEWLEELGGGLIALSGADGGAVGQALLAGDPARAGEAARRLAGL
                     FPGRFYLELQRAGLPTNETHVRAAVPLAASLELPVVATHPVQFLEPDDFDAHEARVCI
                     AEGETLANPRRVKRFTREQYFKSQARMEALWADVPSAVANTVEIARRCNLTLAMGKNY
                     LPDFPTPELADGSKMPMDDYFRQLSYEGLEERLALLFPDAAMRDAKRPEYVERLEFEI
                     NTILKMGFPGYFLIVSDFIKWAKGNGCPVGPGRGSGAGSLVAYVLKITDLDPLHYKLL
                     FERFLNPDRVSMPDFDVDFCQGNRDRVIEYVKDRYGREAVSQIATFGTMAAKAALRDV
                     GRVLGMGYGHVDSIAKLIPAPPGKTVTLAKVPEKPDPGLIYARKEEPEIERREAAEEE
                     VAALLELAARVEGMVRNIGMHAGGVLIAPGKITDFCPLYQQPGSESAVSQFDKDDVEA
                     IGLVKFDFLGLATLTILELAKEFIVARRPDRADFAFETIPLDDAKVYKLFSDGLTEAV
                     FQFESRGMQGMLRDAKPSRLEDLIALNALYRPGPMDLIPTFVARKHGKEEVEYPHPLV
                     EPVLSETYGIMVYQEQVMQTAQVLGGYSLGGADLLRRAMGKKKVEEMAKHREIFREGA
                     AKNGIDQAKADEIFDLMEKFAGYGFNKSHAAAYSLLAYHTGWLKVHCTAEFYAANMTI
                     EMGDTDKLKVLLTDAKQFGIAFVPPDVNICVHRFEPAPGREGDKLIRYGLGAVKGTGQ
                     GAIEAIVAAREGKTELSPTPGPFRSLFDFAKRVDRSRVNKRVVEALVKAGAFDQLHPD
                     RASTLASVSLAFEWADAQAAHAQQGGLFDFGGEDMHGASTQEPALVPVEPWSVRERLT
                     LEKTAIGFFLSGHLFDQNESEVRRFCKRRIVDLIDSREPQTLAGIVSDLRIINGQRGR
                     VGIFKLDDKTDSIEAVANEELLDLHRDALRDDELLVVMGKVQPDRFSGGLRLNVTQIW
                     DLAAARARFARYLSVMADGNVAPLAELVKQWPAKRSVTEHGESVQGLGVRVKVRVDGA
                     AAELDLGDQGRFWPSDDALARFRQILPKDVPQVVYD"
     gene            4626..>5257
                     /locus_tag="VF453_08315"
     CDS             4626..>5257
                     /locus_tag="VF453_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687696.1"
                     /translation="MKRSAIAAAVLALGAVFGACDVAQAAVSVSVIVDPGVYGRVAIG
                     GYVEPPQVYADAPVMVDEDGNLVSVVEPEAYAEDGSDDVDDAGWAPAYPEPVFLWVPE
                     YQRLHWAQYCDLYGARGVPVYFVQDGWYRDNVMHRHWTPAQRGWSRAQWMEQDRLARL
                     HWEHERWERWHHDGGYGGDGGHAIVGVRPEWPGAPGHDRRPQPWPASDGGR"
BASE COUNT          796 a         1865 c         1856 g          740 t
ORIGIN      
        1 ctcggtgttg tcgacggctt gcgcgcgcgc ttcgacgtcg tgcaatacgg ccggctcgac
       61 tacgcgccgg aaacgttccc gctgctggcg atccgcaact gcgaatggcg cgacgacctg
      121 ccttgcgcgc tcgtcaccgg cggcgtgcat ggctacgaga cgagcggcgt gcacggcgcg
      181 ctcgagtgga tggcgcgcgc gttcgaccgc cgcgccggcg cgctcaacct gctcgtcctg
      241 ccctgcatca gcccgtgggg ctacgagacg atcaaccgct ggaatcccga cgcgctcgac
      301 cccaaccggc agttccgcgc cggcagcccg gccgccgagt cggcgctcgc gatggcctgc
      361 gtggccgcgc acggcgggcg ggtcgacctc cacgtcgacc tccacgagac caccgacacc
      421 gacaacagcg agttcggccc cgccaaggcc gcgcgcgacg gcaccgcgtt cgactggcac
      481 gcgatccccg acggcttcta cctcgtcggc gacagcgagc gtccggcgcc ggagttccag
      541 cgcgcgctga tcgaggcggt gcgcggcgtc acgcacatcg ccggggcgga cgagcgcggc
      601 cgcctcatcg gcacgcccct ggcgcagccc ggcgtcatca actaccccaa gcgatccctc
      661 gggctgtgcg gcggcatgac cgccgcgcgc ttcgtgacga ccaccgaggt ctaccccgac
      721 agcgcttcca cgagtcccgc ggaatgcatc gcggcgcagg tggcgacggt cgaggcggcg
      781 atcgagttcc tcctgcgcgg ctgagcgcgt cgcggcgcgc atttcgcccg cgaaaccccg
      841 cgatccgcgc cgggcggcgc cggggcggct ctctacaatc cgtccatggc tttcgttcac
      901 ctgcgcaccc acaccgagtt ctccgtcgtc gacggcaccc ttcgcatcga cgacgtggcg
      961 gcggcggcca aggccgacgg gcaggtggcg ctgggcatca ccgacctgtc gaacctgttc
     1021 ggcgccgtca agttctacaa ggcctgccgc ggcaagggcg tgaagccgat cgtcggcgtg
     1081 gacgtctgga tggatccgct gccggacgcc gccgaccgcg gcgcgacgcg gctcgtgctg
     1141 ctggtgcagg accgccaggg ctacctcaac ctgtgcgaga tgctgggccg cagctggacg
     1201 cgcaacgtgc agcgggcgca ggcgtgggtc aggtgggagt ggctggagga gctgggcggc
     1261 gggctgatcg cgctgtccgg cgctgacggc ggggcggtcg gccaggcgct gctcgcgggc
     1321 gatcccgcgc gcgcgggcga ggccgcacgc aggctggccg ggctgttccc cggccgcttc
     1381 tacctcgagc tgcagcgcgc cggcctgccc accaacgaga cgcacgtgcg cgccgccgtg
     1441 ccgctggcgg cgtcgctgga gctgccggtg gtggccaccc atccggtgca gttcctcgag
     1501 ccggacgact tcgacgccca cgaggcgcgc gtgtgcatcg cggagggcga gacgctcgcg
     1561 aacccgcgcc gcgtcaagcg cttcacgcgc gagcagtact tcaagtcgca ggcccggatg
     1621 gaggcgctgt gggcggacgt gccctcggcc gtcgccaaca cggtggagat cgcgcgccgc
     1681 tgcaacctga cgctggcgat gggcaagaac tacctgcccg acttcccgac gcccgagctc
     1741 gcggacggct cgaagatgcc gatggacgac tacttccggc agctgagcta cgaaggcctc
     1801 gaggaacgcc tcgcgctgct gttccccgac gccgcgatgc gcgacgcgaa gcggccggag
     1861 tacgtcgagc gcctcgagtt cgagatcaac acgatcctga agatgggctt ccccggctac
     1921 ttcctgatcg tgtcggactt catcaagtgg gccaagggca atggctgccc cgtcgggccg
     1981 ggccggggct ccggcgcggg ctcgctggtg gcctacgtgc tcaagatcac cgacctggat
     2041 ccgctgcact acaagctgct gttcgagcgc ttcctgaacc cggaccgggt ctcgatgccc
     2101 gacttcgacg tcgacttctg ccagggcaac cgcgaccgcg tcatcgaata cgtgaaggac
     2161 cgctacgggc gcgaggcggt cagccagatc gcgaccttcg gcaccatggc ggccaaggcc
     2221 gcgctgcgcg acgtcggccg cgtgctgggc atgggctacg gccacgtcga ctcgatcgcc
     2281 aagctgatcc ccgcgccgcc cggcaagacg gtgacgctcg ccaaggtgcc cgagaagccg
     2341 gaccccggcc tgatctacgc ccgcaaggaa gagcccgaga tcgagcgccg cgaggccgcc
     2401 gaggaggagg tcgcggcgct gctggagctg gccgcgcgcg tcgagggcat ggtgcgcaac
     2461 atcggcatgc acgccggcgg cgtgctgatc gcgcccggca agatcaccga cttctgcccg
     2521 ctgtaccagc agcccggcag cgagtcggcc gtgagccagt tcgacaagga cgacgtcgag
     2581 gcgatcggcc tcgtgaagtt cgacttcctg ggcctcgcga cgctgacgat cctcgagctc
     2641 gccaaggagt tcatcgtcgc gcgccggccc gaccgcgcgg acttcgcctt cgagacgatc
     2701 ccgctcgacg acgccaaggt ctacaagctg ttctccgacg gcctgacgga ggcggtgttc
     2761 cagttcgaat cccgcggcat gcagggcatg ctgcgcgacg ccaagccgag ccggctcgag
     2821 gacctcatcg cgctgaacgc gctctaccgc ccgggcccga tggacctgat cccgaccttc
     2881 gtcgcgcgca agcacggcaa ggaggaggtc gagtacccgc acccgctggt cgagcccgtg
     2941 ctgtccgaga cctacggcat catggtctac caggagcagg tcatgcagac cgcccaggtg
     3001 ctcgggggct actcgctcgg cggcgccgac ctgcttcgcc gcgcgatggg caagaagaag
     3061 gtcgaggaga tggccaagca tcgcgagatc ttccgcgagg gcgcggccaa gaacggcatc
     3121 gaccaggcca aggccgacga gatcttcgac ctgatggaga agttcgcggg ctacggcttc
     3181 aacaagtcgc acgcggccgc gtactccctg ctcgcgtatc acaccggttg gctgaaggtg
     3241 cactgcaccg ccgagttcta cgcggccaac atgacgatcg aaatgggcga caccgacaag
     3301 ctcaaggtgc tgctcacgga cgcgaagcag ttcggcatcg ccttcgtccc gcccgacgtc
     3361 aacatctgcg tccaccgctt cgagccggcg ccgggccgcg agggcgacaa gctgatccgc
     3421 tacggcctgg gcgcggtcaa gggcacgggc cagggcgcca tcgaggccat cgtcgccgcg
     3481 cgcgagggca agaccgagct gagcccgacg cccggcccgt tccgcagcct gttcgacttc
     3541 gccaagcgcg tcgatcgctc gcgcgtgaac aagcgcgtgg tcgaggcgct ggtcaaggcc
     3601 ggggcgttcg accagctcca tcccgaccgc gcgagcacgc tcgccagcgt ctcgctcgcc
     3661 ttcgagtggg ccgacgccca ggccgcgcac gcgcagcagg gcggcctgtt cgacttcggc
     3721 ggcgaggaca tgcacggcgc ctccacgcag gagcccgcgc tggtgccggt cgagccctgg
     3781 agcgtgcgcg agcgcctgac cctggagaag acggcgatcg gcttcttcct ctcgggccac
     3841 ctcttcgacc agaacgagtc cgaggtgagg cgcttctgca agcgccgcat cgtcgacctg
     3901 atcgattcgc gcgagccgca gacgcttgcc gggatcgtca gcgacctgcg catcatcaac
     3961 ggccagcgcg ggcgcgtggg catcttcaag ctggacgaca agaccgactc gatcgaggcg
     4021 gtcgccaacg aggaattgct cgacctccac cgcgacgcgt tgcgcgacga cgagctgctg
     4081 gtcgtgatgg gcaaggtgca gcccgaccgc ttcagcggcg gcctgcgcct gaacgtcacg
     4141 cagatctggg acctggcggc ggcgcgcgcg cgcttcgccc gctacctctc ggtgatggcc
     4201 gacggcaacg tcgccccgct ggccgagctc gtgaagcagt ggccggccaa gcgctcggtg
     4261 accgagcacg gcgagtcggt gcagggcctg ggcgtgcgcg tcaaggtgcg cgtcgacggc
     4321 gccgccgccg agctcgacct cggcgaccag ggccgcttct ggcccagcga cgacgcgctg
     4381 gcgcggttca ggcagatcct gcccaaggac gtgccgcagg tcgtctacga ctgaagccgc
     4441 ggtcactgcg gggccgggtc gaggccggcg acggtcgggc tggcgctggg cgggaacggc
     4501 ccgggccgcc ccaggatgca ggcacgcttt gtttccggtc tttaccttcg cgacgccacg
     4561 accctgacaa cttgcggcaa gctgaacgcg ttgaggaggg gcaacaccac tttctggagt
     4621 gcctcatgaa acgttccgcg atcgctgctg ccgtgctggc cctgggcgcc gtcttcggcg
     4681 cgtgcgacgt cgcgcaggcc gcggtgtcgg tgtcggtcat cgtcgacccg ggcgtctacg
     4741 gccgcgtcgc catcggcggc tatgtcgagc cgccgcaggt gtacgccgac gcgccggtga
     4801 tggtcgacga ggacggcaac ctggtcagcg tcgtcgagcc cgaggcgtac gccgaggacg
     4861 gcagcgacga cgtcgacgac gccggctggg cgcccgcgta tccggagccc gtcttcctgt
     4921 gggtgcccga gtaccagcgc ctgcattggg cgcagtactg cgacctgtac ggcgcgcgcg
     4981 gcgtgccggt ctacttcgta caggacggct ggtaccgcga caacgtgatg caccgccact
     5041 ggacgccggc gcagcgcggc tggagccgcg cgcagtggat ggagcaggat cgcctcgcgc
     5101 gcctgcactg ggagcacgag cgctgggaac gctggcacca cgacggcggc tacggcggtg
     5161 acggcggtca cgcgatcgtc ggcgttcgtc ctgaatggcc gggggctccc ggccacgacc
     5221 gccgcccgca gccctggccg gcgagcgacg gcggccg
//
LOCUS       DASVLN010000338         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_448246, whole genome shotgun sequence.
ACCESSION   DASVLN010000338 DASVLN010000000
VERSION     DASVLN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448246"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..>2149
                     /locus_tag="VF453_08320"
     CDS             38..>2149
                     /locus_tag="VF453_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687697.1"
                     /translation="MTSARVDTGEAAAAAAPAAGAEYPAFPRTLADFEAQLKAPPKPI
                     DRRKAAALINKFRPNPARPGSGLAQLSARQVATWEREVMGMSRAERKRRRDVVFLSEL
                     PYPTSSSVVNGIIYCAVPFIDMSAKGHAVTALLVAGLSPIAVAAIQVMTNKAAEVYRQ
                     KNSMATKVDKSRIHARLTPSQMLEEQASALAALHANNDRLDTFVTDMAAKHGVSFAEP
                     RGADAFDDATRAALHEIVPPTRHARLRLALRAAAEGEDSTALRALVDGLPAGDRDRAD
                     KVVADALGRHYQARMDELAGKLSKEELLELTGLFVDNASHELRIHELTEGLDMSKELR
                     DRNLVYTGWQGPARILRAGSGVVKTLLRQPGKSAPASWLDGWWPARRLPLGAAKGIAA
                     GVAGAALVTQHVLCGFDGVNAFHRDNRMNIERGDLFNEAGHLAWARGERATEDFFDEE
                     KMHAMWPREVVQMRDRTVKLVEGWLERLEAQEQALAGAGAPAADGTDLEPGGEHPSPQ
                     ARQLAATIAEYRNDLALLKSGDVTELTRGGDVRRILDDIMAGTSWRHAVREGWNALTP
                     EEYSAQIGQRYTQAFMGGVFGAVGAAAAGPVLSWLLGGDGIPLSTELYIGLGGAALGA
                     VSAYTQSMAVDGKNQRRDGGTMGLGGQLLNGLVQPAVAAKQRLDIHWGRPAAAARSRQ
                     AAGVAGSRLQSAVGQAIDKRLD"
BASE COUNT          329 a          750 c          809 g          261 t
ORIGIN      
        1 gaacgccctg cggcccaccc gcgcgtcgcg ggaggccatg acctcggcgc gcgtcgacac
       61 cggcgaggcg gcggcggccg cggccccggc cgccggggcc gagtacccgg cgtttcctcg
      121 caccctcgcg gacttcgagg cgcagctcaa ggcgccgccc aagcccatcg accggaggaa
      181 ggccgcggcc ctgatcaaca agttccggcc caacccggca cgtcccggct cggggctggc
      241 gcagctgtcg gcgcgtcagg tcgcgacctg ggagcgcgag gtgatgggca tgagccgcgc
      301 ggagcgcaag cggcggcggg atgtcgtctt cctgtccgag ctgccctatc cgaccagttc
      361 gagcgtcgtc aacggcatca tctactgcgc cgtgccgttc atcgacatgt ccgcgaaggg
      421 gcatgcggtc acggcgctgc tcgtcgcggg actttcgccc atcgccgtcg cggccatcca
      481 ggtgatgacc aacaaggcgg ccgaggtgta ccgccagaag aactcgatgg ccacgaaggt
      541 cgacaagtcg cgcatccacg ccaggctcac gccgtcgcag atgctcgagg agcaggcgtc
      601 ggcactggcg gcgctgcacg cgaacaacga caggctggac acgttcgtca ccgacatggc
      661 ggcgaagcac ggcgtgtcgt ttgccgagcc gcgcggcgcc gacgccttcg acgacgccac
      721 gcgcgccgcg ctgcacgaga tcgtcccgcc gacacgccac gcgcgacttc gcctggcgct
      781 gcgggccgcg gccgagggcg aggactcgac ggcgctgcgc gccctggtgg acggcctgcc
      841 ggccggcgac cgcgatcgcg ccgacaaggt cgtcgcggac gcgctcggcc gccactacca
      901 ggccaggatg gacgagctcg cgggcaagct ctcgaaggag gagttgctgg agctcaccgg
      961 cctgttcgtc gacaacgcgt cgcacgagct gcgcatccac gagctgaccg aaggcctcga
     1021 catgagcaag gagctgcgcg accgcaatct cgtgtatacg ggatggcagg ggccggcgcg
     1081 gatcctgcgg gccggcagcg gcgtggtcaa gaccctgctg cgccagccgg gaaaaagcgc
     1141 gcccgcgagc tggctggacg gatggtggcc cgccaggcgc ctgccgctgg gggcggccaa
     1201 gggcatcgcc gcgggcgtcg ccggcgcggc gctcgtgacg cagcacgtgc tgtgcggctt
     1261 cgacggcgtc aacgccttcc accgcgacaa ccgcatgaac atcgagcgcg gcgacctgtt
     1321 caacgaagcg ggccacctgg cgtgggcgcg gggcgagcgg gcgaccgagg acttcttcga
     1381 cgaggagaag atgcacgcga tgtggccgcg ggaggtcgtg cagatgcgcg atcgcacggt
     1441 caagctcgtc gagggctggc tcgagaggct ggaggcacag gaacaagcgc tggccggtgc
     1501 cggtgcgccc gccgccgacg gcacggacct ggagcccggc ggggagcatc cgtcgccgca
     1561 ggcccgccag cttgccgcca ccatcgcgga gtaccggaac gacctggcgc tgctgaagtc
     1621 cggcgacgtg accgagctga cgcggggcgg ggacgtccgg cgcatcctcg acgacatcat
     1681 ggcgggcacc tcctggcgcc atgccgtgcg cgaaggatgg aacgcgctca ccccggagga
     1741 atattcggcc cagatcggcc agcgctacac gcaggccttc atgggcggcg tcttcggcgc
     1801 ggtcggggcg gccgccgccg ggcccgtcct gtcgtggttg ctgggcggcg acggcatccc
     1861 gctgtccacg gagctctaca tcggcctggg cggggcggcc ctcggggccg tctcggcgta
     1921 cacgcagtcg atggcggtgg acggcaagaa ccagcgccgc gacggcggca cgatgggcct
     1981 gggcgggcag ctcctgaacg gactggtgca gcccgccgtg gcggcgaagc agcgcctcga
     2041 catccactgg ggacggccgg cggcagcggc gcgttcgcga caggccgcgg gcgtggccgg
     2101 cagccggctg cagtccgcgg tgggacaggc catcgacaag cgcctggac
//
LOCUS       DASVLN010000339         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_179159, whole genome shotgun sequence.
ACCESSION   DASVLN010000339 DASVLN010000000
VERSION     DASVLN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179159"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..581)
                     /gene="rplF"
                     /locus_tag="VF453_08325"
     CDS             complement(48..581)
                     /gene="rplF"
                     /locus_tag="VF453_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7687698.1"
                     /translation="MSRVGKMPITVPQGVDVSIAEDKITVKGASGTLTLPANKLVEVK
                     KDGAVVTFAPADDSQEANAMSGTFRALVNNMVLGVSKGFEKKLTLVGVGFRAQAQGQK
                     LNLQIGFSHPVVKDMPAGITVTTPTQTEVLIKGADRQVVGQIAAEVRAIRPPEPYKGK
                     GIRYADERVVLKETKKK"
     gene            complement(595..990)
                     /gene="rpsH"
                     /locus_tag="VF453_08330"
     CDS             complement(595..990)
                     /gene="rpsH"
                     /locus_tag="VF453_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7687699.1"
                     /translation="MSMSDPIADMLTRIRNAQSVEKPVVSMPSSKLKVAIAQVLKDEG
                     YIDGFAVKADGAKSELEISLKYYAGRPVIERIERVSRPGLRIYRGRDAIPQVMNGLGV
                     AIVTTPKGVMTDRKARQTGVGGEVLCYVA"
     gene            complement(1015..1320)
                     /gene="rpsN"
                     /locus_tag="VF453_08335"
     CDS             complement(1015..1320)
                     /gene="rpsN"
                     /locus_tag="VF453_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX7687700.1"
                     /translation="MAKTSLIQREAKREATVAKFAKKRAELQAVIGDAKRSDEERYIA
                     RLELQKLPRNSAPTRLRNRCTLTGRPRGTFRTFGLARNKIRELAFKGDIPGVIKASW"
     gene            complement(1329..1868)
                     /gene="rplE"
                     /locus_tag="VF453_08340"
     CDS             complement(1329..1868)
                     /gene="rplE"
                     /locus_tag="VF453_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7687701.1"
                     /translation="MARLQEHYREKIVPELTAKFGYKSVMEVPRLTKITLNMGVSEAV
                     ADKKVMDHAVGDLTKIAGQKPVVTKSKKAIAGFKIRDGVPIGCMVTLRGVQMYEFLDR
                     FVTIALPRVRDFRGISGRSFDGRGNYNIGVKEQIIFPEIEYDKIDAIRGMNISITTTA
                     KSDDECKALLSAFKFPFKN"
     gene            complement(1879..2196)
                     /gene="rplX"
                     /locus_tag="VF453_08345"
     CDS             complement(1879..2196)
                     /gene="rplX"
                     /locus_tag="VF453_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX7687702.1"
                     /translation="MNKIRKGDQVIVTTGRDKGKRGTVTERVSEQLVVVEGINVAKKH
                     VKPNPLKGTTGGVVEKTMPIQQSNIAIFNPATGKADRVGIKILADGKKVRVFKSSGEE
                     VKA"
     gene            complement(2226..2594)
                     /gene="rplN"
                     /locus_tag="VF453_08350"
     CDS             complement(2226..2594)
                     /gene="rplN"
                     /locus_tag="VF453_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX7687703.1"
                     /translation="MIQMQSRLDVADNTGAKSVMCIKVLGGSKRRYAAIGDVIKVSIK
                     EAAPRGRVKKGEVYNAVVVRTAKGVRRQDGSLVKFDGNAAVLLNAKLEPIGTRIFGPV
                     TRELRSERFMKIVSLAPEVL"
BASE COUNT          381 a          841 c          860 g          575 t
ORIGIN      
        1 cgaacggcgc aggcgctgtt ccttcttgtt cagcatgttg gcgctcctta cttcttcttc
       61 gtctctttca gcaccacgcg ctcatcggcg tagcggatgc ccttgccctt gtacggctcg
      121 ggcggacgga tggcacgcac ctcggcggcg atctggccga cgacctggcg gtcggcgccc
      181 ttgatcagca cttcggtctg cgtcggggtc gtcacggtga tgccggcggg catgtccttg
      241 acgaccgggt gcgagaagcc gatctgcagg ttgagcttct ggccttgcgc ctgggcacgg
      301 aagccgacgc ccaccagcgt gagcttcttc tcgaagccct tgctgacgcc cagcaccatg
      361 ttgttcacca gggcgcggaa cgtgccgctc atggcgttgg cttcctggct gtcgtcagcc
      421 ggggcgaacg tcacgacggc gccgtccttc ttgacctcga ccagcttgtt cgccggcagc
      481 gtgagggtgc ccgaggcgcc cttcacggtg atcttgtctt cggcgatcga cacgtccacg
      541 ccttgcggga cggtgatcgg catttttcct acgcgggaca ttgcggcaat ctccttacgc
      601 gacgtagcac aggacttcgc cgcccacgcc ggtctggcgg gccttgcggt cggtcatcac
      661 gcccttcggg gtcgtgacga tcgcgacgcc caggccgttc atcacctgcg ggatcgcgtc
      721 gcggccgcgg tagatgcgca ggccaggacg gctcacgcgc tcgatgcgct cgatgaccgg
      781 gcgaccggcg tagtacttca gcgagatttc cagttcggac ttcgcgccat cggccttgac
      841 ggcgaagccg tcgatgtagc cctcgtcctt caggacctgg gcgatagcga ccttcagctt
      901 cgacgaaggc atgctgacga ccggcttctc aacgctttgc gcgttgcgga tgcgggtcag
      961 catgtcggcg ataggatcac tcatgctcat gctgttttct cctgtctcgc ctgattacca
     1021 gctggccttg atcacgcccg ggatgtcgcc cttgaaggcg agttcgcgga tcttgttgcg
     1081 cgcgaggccg aacgtgcgga acgtgccgcg gggacggccc gtgagcgtgc agcggttgcg
     1141 caggcgcgtc ggcgccgaat tgcgcggcag cttctgcagc tcgaggcggg cgatgtagcg
     1201 ctcttcgtcg ctgcgcttgg cgtcgccgat gacggcctgc agctcggcgc gcttcttcgc
     1261 gaacttcgcg acggtcgctt cgcgcttggc ttcgcgctgg atgagggaag tctttgccat
     1321 ggggcgcctc agttcttgaa cgggaacttg aacgccgaca gcagtgcctt gcactcgtcg
     1381 tcgctcttgg ccgtggtggt gatcgagatg ttcatgccac ggatcgcgtc gatcttgtcg
     1441 tactcgattt ccgggaagat gatctgctct ttcacgccga tgttgtagtt gccgcggccg
     1501 tcgaacgagc ggcccgagat gccacggaag tcgcgcacgc gcggcagggc gatcgtgacg
     1561 aagcggtcca ggaattcgta catctggacg ccgcgcaggg tgaccatgca gccgatgggc
     1621 acgccgtcac ggatcttgaa gccggcgatg gccttcttcg acttggtgac cacgggcttc
     1681 tggccggcga tcttggtcag gtcgccgacg gcgtgatcca tgaccttctt gtcggcgacg
     1741 gcctcgctca cgcccatgtt cagcgtgatc ttggtgaggc gcggcacttc catcacggac
     1801 ttgtagccga acttggccgt gagttccggg acgatctttt cgcggtagtg ttcttgcaga
     1861 cgagccatgc cggacctctt atgccttgac ttcttcgccg ctggacttga agacgcgaac
     1921 cttcttgccg tcggcgagga tcttgatgcc cacgcggtcg gccttgccgg tcgccgggtt
     1981 gaagatcgcg atgttggact gctggatcgg catcgtcttc tcgacgacac cgcccgtcgt
     2041 gcccttcagc gggttcggct tcacgtgctt cttggcgacg ttgatgccct cgacgacgac
     2101 cagctgctcg ctgacgcgct cggtgacggt accgcgctta cccttgtcgc ggccggtggt
     2161 cacgatgacc tggtcgccct tgcggatctt gttcatggcg tttccttaga ctgcgggacg
     2221 gcgcttcaga gcacttccgg cgcgagcgac acgatcttca tgaagcgctc ggaacgcagc
     2281 tcgcgcgtca cgggcccgaa gatgcgggtg ccgatgggct cgagcttggc gttcagcagc
     2341 acggcggcgt tgccgtcgaa cttcaccagc gagccgtctt ggcggcgaac gcccttggcg
     2401 gtgcggacga cgacggcgtt gtagacctcg cccttcttca cgcgaccgcg cggcgcagct
     2461 tccttgatgc tgaccttgat gacgtcacca atggccgcat agcgacgctt ggagccaccg
     2521 agcaccttga tgcacatgac ggacttcgcg cccgtgttgt cggcgacgtc gagtcgcgac
     2581 tgcatttgaa tcatgtttta tccccaactt gctcccgcag acggcaaccc gagagcggtc
     2641 agtattgggc ccgtcgg
//
LOCUS       DASVLN010000340        11172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_448262, whole genome shotgun sequence.
ACCESSION   DASVLN010000340 DASVLN010000000
VERSION     DASVLN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11172
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448262"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..552
                     /locus_tag="VF453_08355"
     CDS             151..552
                     /locus_tag="VF453_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687704.1"
                     /translation="MADSNSKSSHEPANKVDDAFAEDLLEKVHSCAPILAEADVELIE
                     HRMQSADFGIGRRIGLVTASKDGAWFKSVSTGTDHALAVGAMSAMHGIREHIERMREL
                     TNWLESAEIRLMVALAAREDYQELEAEVLNG"
     gene            545..1036
                     /locus_tag="VF453_08360"
     CDS             545..1036
                     /locus_tag="VF453_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687705.1"
                     /translation="MGDLLKHPASDGPRVENIRAPGRKKGTVSLRTERFKRCAAAQPP
                     AEPTPDEVRLPPIEAGKFFYPKSMLTQAYFCRGGTGIWSSPSMSLGMLVNERFVSPMG
                     ALLEQLEAEGHNVAGARAEWLAFRELAKGLKGDFDALSQQVMRIQMRRPLATAMLLED
                     GSK"
     gene            1206..2753
                     /locus_tag="VF453_08365"
     CDS             1206..2753
                     /locus_tag="VF453_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687706.1"
                     /translation="MDVALEAAALRFPMPAEGDEDARTLALEWQRWATETLLRQANGY
                     AHDGKFGIVLADGTTLATRDVFSVPQENWRVTASERETALKLFKDDPVHIRPIFYMPV
                     RPAEVPPALKLLPRDTSVSLRWTWRRFSESVGPAVDAIEAFEATLRRQAQGWLVIEEA
                     VHELQAAGRGTAKTWTEKLEEAAQSGDLPMHEPESLERVSYKPTRQRPEPRRVRDFYD
                     WVHVDDLNAWLRAHEPRLPYQFESPANQDYAAKPRQVQANNAVVVSLAGERRTRREAP
                     TDSPFDGLSGVLYVRAGEQLFALRDIPEMTARALVPGASTAVLPSPSSSGISEDDEGA
                     TFRLLFALVLDDHRRRLNAALADRTLISTWGRLPPAPELQEVLPLGELRQFCESIRVE
                     VAVLNDDEAREATPVIAISHEPATKDVDAHPAISVSHVLSSRRDVLDPIIDLAERNAT
                     NPDDRAAVWNAFAALAVSASKPPPLLGFADRSIQYLDGDEAKFLTRDAFNKRWERRKG
                     RAAGSRR"
     gene            2848..3078
                     /locus_tag="VF453_08370"
     CDS             2848..3078
                     /locus_tag="VF453_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEX7687707.1"
                     /translation="MTSHTATAFDNLPATGYVRQSQLIPAPVPFSSATLWRKCKSGDF
                     PKPIKLSARVTAWKVEDVRQWLAAQAAKGGAA"
     gene            3075..3440
                     /locus_tag="VF453_08375"
     CDS             3075..3440
                     /locus_tag="VF453_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687708.1"
                     /translation="MKKAPATYQADGASGEQLAFIDPPPFCPSWPKRNSLADRALKLF
                     LDGKVFDHPDFLGACGSWRLAAVVFQLRALGWPIDTIEIPSPSEEHPGRTIALYKLPP
                     KYAAEAFALMTGGANACRR"
     gene            3424..5496
                     /locus_tag="VF453_08380"
     CDS             3424..5496
                     /locus_tag="VF453_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7687709.1"
                     /translation="MLVDVERARDALLACDPGCSREEWVRLAMAAKAAGVDLDTFHSW
                     SAQASNYDERDARSVWTSIRRADGIGPGTLFKAAAQNGWSPSSRAVRSKQPQDAKRPA
                     EHRRRVRPGMDAAAVWERSVPASDSHGYIAAKQGVPDGLRVLPQDDLLTIAGQRMAGA
                     LVVPVNSLDGTLSSLQFIPAPGAGKQLNLPGASIDGVFVVGALRNGAVAYLCEGIGQA
                     WACWKATGQAAIVCFGWGRVRAVASELRKRDPAARLVIVPDVGKEPDAEAVAREVCGA
                     CALMPGGWGANSDVNDFAQREGFDALEAMLQGARAPLADGVVLLNGADLVPQPVSWLW
                     RDWLALGKLHILAGAPGQGKTTIAIAMAATVTSGGRWPDGSRSERGHVLVWSGEDDPA
                     DTLLPRLLAAGGDKSRVHFVSGTRVNGKMQPFDPATDMAQLAAQATRIGDVRLIIVDP
                     VVSAVTGDSHKNTEVRRALQPLVDLASAMGSALVGISHFSKGGQGQDPASRVVGSVAF
                     TAVARVVLVAAKVKGDDGQDRRILARGKSNIGPDDGGFEYAISQVEALPGIRAARIEW
                     GSSVEGTARELLTDPAEEGEENNALDSAAAFLREVLGDDLVPVKSIEAEAKDAGIAWR
                     TIRRASDSLAVVKRRGMENRWYWSLPKLSKPGNLSNLSNVSSLDNLANMDTPPVGKSA
                     ENDEGSAR"
     gene            5493..5840
                     /locus_tag="VF453_08385"
     CDS             5493..5840
                     /locus_tag="VF453_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7687710.1"
                     /translation="MSVIALVTGKLLKAPTTRSGRSGDPFTLATMKAATDDGDLLVNL
                     IGFGLAGEILAKLGVGDALSVTGRAKLNHWTANDGQERHGLNVVVDGVLTPYQVNVRK
                     RRVQGGKEEEAES"
     gene            5837..6532
                     /locus_tag="VF453_08390"
     CDS             5837..6532
                     /locus_tag="VF453_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687711.1"
                     /translation="MTRGATPKAKRKPSAEVEKTPQRRTAVVVECRSVADNYPAVART
                     LSRPEVSAAATIEKWQPDTHDVNDLASELAAQVSAVNSGDLGRAEAMLIAQAHTLNEV
                     FNNLLRRAMNQTGLPQWEAYMRMGMKAQSQCRTTLEALAEIKNPRAVAFVRQANIAHG
                     PQQVNNAAPTASPDQYMQAGSGAQKQENEPSKLLEQQHGQWLDAGAQSSASGADKAVA
                     PLGTRDGSRIGRR"
     gene            complement(6763..7239)
                     /locus_tag="VF453_08395"
     CDS             complement(6763..7239)
                     /locus_tag="VF453_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687712.1"
                     /translation="MAAVESAFLRLVRLAVGGGLLWGAYEAAAWGWPALDKPFAALTI
                     RDLLTLVLGLALAAMLAPSALAVAFGAGSKTPQPSIEDAARGNVMHRLKMQEREAATQ
                     QAERARLASWYSRSKWLGVLFDPSIARKHRWLPFVAVLGAYALLALVDYVVSHLSP"
     gene            complement(7718..7805)
                     /locus_tag="VF453_08400"
     tRNA            complement(7718..7805)
                     /locus_tag="VF453_08400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7769..7771),aa:Ser,seq:cga)
     gene            complement(7935..8504)
                     /locus_tag="VF453_08405"
     CDS             complement(7935..8504)
                     /locus_tag="VF453_08405"
                     /inference="COORDINATES: protein motif:HMM:NF018924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HEX7687713.1"
                     /translation="MNGRRAAMAVGGALAAGALAWWAIVAFVPEATLDIDEAQLQARL
                     DARFPQRNCALMVACLTLSDPKVTLTEGSDRIGLAADVLVSLGHRTASGKVAFSGTLR
                     YVRYRGEFYLDDVRIDSFQLVGFPSELVEVVKERGPSVLRRALEGHPIYTIRADSPQA
                     ALAKLAVRDARVVDGKVRVTFLRFGMGGG"
     gene            complement(8501..9001)
                     /locus_tag="VF453_08410"
     CDS             complement(8501..9001)
                     /locus_tag="VF453_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687714.1"
                     /translation="MPTSPTNPPPDSPLKGKPPVQGDVAGTPVQAIVVGLVIDIVGST
                     VASLALRGIYQLGLMHAGLSDDEVRRTLDDLGPSSVVGVIEMLAGWACSVAGGYACAR
                     VVMRDEFRFGAIMVVLSCLITGGDGPLDLQLLDIACEAVCVMLGVMYGVRRNRRPPPP
                     PGASAP"
     gene            complement(9090..>11172)
                     /locus_tag="VF453_08415"
     CDS             complement(9090..>11172)
                     /locus_tag="VF453_08415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7687715.1"
                     /translation="RLIPGRGRARVVATLDGERLGVQRDLEREREHLDSILDACPMLV
                     PLAPDAPCEWQLDAADDALALLERLQPHHAIAALDWPQGRPVRVLPGALDNLVLKVRT
                     HEDWLGLQGELKVDERLVVSLQQLIGFAVAQRSRFVPLGDGRYLALSQALRERVADIA
                     AVVEAQAARLDSVEEMRLPTVAAPWLQAASEGLHVEHDAAYAARLMALDEARRIVPKL
                     PGALQAELRPYQEEGFEWAMRLAAAGLGACLADDMGLGKTLQAIAVLLARAADGPALV
                     VMPTSLVGNWRSECARFAPTLRVHVYAEQGAAGERANAIGRLGRRDVLLVSYPMLQLD
                     EAAFAAKPWATLVLDEAQAIKNAAAKRSQAVFALQAAFRLAMSGTPIENRLGELWSVM
                     RACNPGLLGSQARFNERFANLIERQHDRAAQRTLKRLIAPFVLRRTKAQVLAELPPRT
                     ELVLRVEGDEAEQAHYEALRREALVAAEKSLSGDGAGQAHLNILAGLTRLRRAACDPR
                     LVTPGLRPGAKVAAFAELAAELVANGHKALVFSQFVDFLALLREPLDAAGIAYQTLDG
                     STPSAERTRRVEAFQAGEGDLFLISLKAGGFGLNLTVADYVVIADPWWNPAAEDQASG
                     RAHRIGQRRPVTVYRMVHAGTLEERILDLHQAKRELAEGMLEADGDPRAAVPLARELL
                     QLMRGDDPPDA"
BASE COUNT         2025 a         3687 c         3764 g         1696 t
ORIGIN      
        1 ccgacagcct caacacgcga ccccgcgcca ctcacgactg gcacacgccg cttgaggtct
       61 tcggactcac cctggcaaac tctcacaagt ctccgagctc agttcaataa cgcggtgttg
      121 cagttcggat ttgaaaccgc caggtgaacc atggctgact ccaactctaa gtcctcccac
      181 gagcccgcga acaaggtcga cgacgcgttc gctgaagacc tgcttgagaa ggtccactcc
      241 tgcgcgccca tcctcgctga ggcggacgtc gaactcatcg agcaccggat gcagtccgca
      301 gacttcggca tcggccgacg catcggcctc gtgaccgcaa gcaaagacgg cgcgtggttc
      361 aagtctgtct ctaccgggac ggaccacgca ctcgccgtcg gtgcgatgtc tgcgatgcac
      421 ggcattcgag agcacatcga gcgaatgcgc gagctgacga actggcttga aagcgctgag
      481 attcggctta tggtcgccct cgccgcgcga gaggactacc aagagctgga agccgaggtg
      541 ctcaatgggt gacctgctca agcatcctgc atcagacggc ccgcgggtgg aaaacatccg
      601 tgctcccgga aggaaaaagg gaaccgtgag tctgcgcacc gagcggttca agcggtgcgc
      661 tgcagcgcag ccgccggcag agccgacacc cgacgaggtg cgccttccgc caatcgaggc
      721 aggcaagttc ttctatccca agtcgatgct gacgcaggcg tatttctgca gaggcggcac
      781 tgggatttgg agctcaccga gcatgtccct cggcatgctg gtcaacgagc gattcgtgtc
      841 tccaatggga gcgcttctgg aacaacttga ggccgaaggg cacaacgtgg ccggagctcg
      901 agcagagtgg ctggcgtttc gagaactggc aaagggtctg aagggcgact ttgacgcgct
      961 gtcccagcaa gtcatgcgaa tccagatgcg acgcccgctc gccaccgcga tgctgctgga
     1021 ggatggctca aagtagcttc taatggtgct gccccagcta gggccggccg caaaccggac
     1081 cgaacgccgc gcgcccccga cgtggcctgc tggggcgctc tcattcgggg tgcatcgggg
     1141 gatgcatgac tgaccaagca agccgggcaa ttccggcttc gtgcgagcca caacttcact
     1201 cagtcgtgga tgttgcgctc gaagctgctg cactgcgatt tccgatgcct gcagaaggcg
     1261 atgaggacgc gcgaacgctc gcgctggaat ggcaacggtg ggcgacggaa actttgctac
     1321 gccaagcgaa cggatatgcc catgacggga agttcggcat tgttttggca gatggaacga
     1381 cactcgccac gcgcgacgtg ttctccgtcc cgcaggaaaa ttggcgagtg actgcgtcgg
     1441 aacgcgagac agccctcaag ctgttcaaag acgaccccgt acacatccgc cccatttttt
     1501 acatgcctgt ccgcccggcc gaagtgccgc cagcgctcaa gctcttgccg cgggacacgt
     1561 ctgtcagcct ccggtggaca tggcgtcggt tttcagagtc cgtcgggccg gcagtcgatg
     1621 ccattgaagc attcgaagcg acccttcggc gccaggcgca gggctggctc gttatcgagg
     1681 aagcggtgca tgagcttcaa gccgctggac gtggaacagc gaaaacctgg acagagaaat
     1741 tggaagaagc ggcacagtcg ggggacttgc cgatgcacga gccggaatca cttgaacgcg
     1801 tgagctacaa gccgacgcga caacggccag agcctcgccg agtacgcgac ttctacgact
     1861 gggtacacgt cgatgacctc aacgcttggc ttcgcgcgca tgagccacgc cttccctacc
     1921 agttcgagag tccagcaaac caagactacg ccgccaaacc tcgacaagtg caggccaaca
     1981 acgctgtcgt tgtgagcttg gcaggcgagc gtcgcacccg aagagaagcg ccgaccgact
     2041 cgcctttcga tgggcttagc ggggtgctct acgtccgagc cggcgagcag ctctttgcgt
     2101 tgcgcgacat accggagatg actgcacgtg cgctcgtccc tggagcctcc acggcagtct
     2161 tgccttcacc gtcatcaagc ggcatcagcg aagacgatga aggggcaacg tttcgactcc
     2221 tcttcgcgct cgtcttggac gaccataggc gaaggctgaa tgcagcgctg gccgacagga
     2281 cactgatttc cacgtggggc cgtttgccgc cggcgccgga gcttcaagag gtgcttccac
     2341 tcggagagct acgccagttc tgcgaatcaa tacgggttga agtggcggtc ttgaacgatg
     2401 acgaagcgcg cgaggcgaca ccagtgatag ccatctcgca cgagccggcg accaaggacg
     2461 tagatgcaca tccggccata tcggtgtctc atgttctctc cagccgtcgc gacgtcctcg
     2521 acccaatcat cgacctggcc gagcgaaacg caacgaatcc ggacgaccgc gccgcagtgt
     2581 ggaacgcgtt tgcagccctg gcagtctcgg cttcgaagcc ccctcccctg cttggattcg
     2641 ctgaccgctc catccagtac ctcgacgggg acgaggcgaa gtttctgacc agggatgcct
     2701 tcaacaagcg atgggagcgg aggaaggggc gggccgccgg cagtcgccgc tagtcgccac
     2761 gggccgctgc gggccgccgc cggtatgtgg aaacgcggcg tcgaaaagga gcatgagcgt
     2821 caccttcaca gcatcgcgtg acgcctcatg acctcccaca ccgcaaccgc attcgacaac
     2881 ctgcccgcca ccggctacgt ccggcagtcg caactcattc cggcaccggt gccgttctcg
     2941 tcggccacgc tctggcgcaa gtgcaagtcc ggcgacttcc ccaagcccat caagctctcc
     3001 gcacgcgtga ccgcttggaa ggtcgaggac gtacggcaat ggctcgccgc ccaagctgcg
     3061 aagggcggtg cagcatgaag aaagccccgg cgacctacca agctgacggg gcttcgggcg
     3121 agcaacttgc gttcatcgac ccgccgccat tctgcccgag ctggccgaag cgcaacagcc
     3181 tcgccgaccg agcgctaaag ctgtttctcg atgggaaggt ctttgaccat cccgatttcc
     3241 tcggtgcctg cggctcgtgg cggctcgccg ccgtggtgtt ccagctgcga gcgcttggct
     3301 ggcccatcga caccatcgag attcccagcc ccagcgagga gcaccccggt cgcaccatcg
     3361 ccctctacaa actcccgccg aagtacgccg cggaggcgtt cgctctgatg acgggaggtg
     3421 ccaatgcttg tcgacgttga gcgcgccaga gacgcactgc tggcgtgcga cccgggctgc
     3481 tcccgagagg aatgggttcg tcttgccatg gcagccaagg ctgccggcgt tgacctggac
     3541 accttccatt cgtggagcgc gcaggccagc aactatgacg agcgcgacgc ccgctctgtc
     3601 tggacgtcga ttcggcgtgc agatggcatc ggcccgggaa ccctgttcaa agctgcagcc
     3661 cagaatggat ggagcccgag cagccgtgct gtccgctcga agcagccgca ggacgccaag
     3721 cggcccgctg agcaccggag acgggttcgg cccgggatgg atgcagcggc ggtctgggaa
     3781 cgcagtgttc cggcgagcga ctcgcacggc tacatcgcgg caaagcaagg cgtccccgac
     3841 ggcttgagag tcttgccgca agacgacctg ctgaccattg ccggacaacg gatggccggc
     3901 gctctggtcg tgccagtgaa cagcctcgac ggcaccctat catctctgca gttcatcccg
     3961 gcgcccgggg caggcaagca gctcaacttg ccgggcgctt ccatcgacgg cgtgttcgtc
     4021 gtcggagcat tgagaaacgg tgccgtcgcc tacctatgcg aaggcatcgg ccaagcatgg
     4081 gcatgctgga aggcaacggg acaggcggca attgtgtgct tcggctgggg gcgtgttcgc
     4141 gctgtcgcga gcgagctgcg caagcgcgac ccggctgccc gacttgtcat cgtgccggac
     4201 gtcggcaaag aacctgacgc cgaggctgtc gctcgcgagg tgtgcggcgc ctgcgcgctc
     4261 atgccgggag gttggggcgc gaactcggac gtcaacgact ttgcccagcg agagggcttc
     4321 gacgcgctgg aggcaatgct gcaaggtgcg agggcgccgc tcgcagacgg ggtcgtgctt
     4381 ctgaatggcg ctgaccttgt cccgcagccg gtgtcgtggc tgtggcgcga ttggctcgcc
     4441 ttggggaagc tgcacatcct ggctggtgcc cctggacagg ggaagacgac cattgccatc
     4501 gccatggctg ccacggtgac gagcggcggg cgttggccgg acggctcacg aagcgagcga
     4561 gggcacgtgc tcgtctggtc aggcgaagac gaccccgccg acacgctgct acctcgcctg
     4621 ctagcggcgg gaggtgacaa gtctcgagtt cactttgtct cggggactcg cgtgaacgga
     4681 aagatgcagc cattcgaccc ggcaaccgat atggcgcaac tcgcagcaca ggcgactcgc
     4741 atcggcgacg ttcgcctcat catcgttgac cccgtggtct cggcggtgac gggcgacagc
     4801 cacaagaaca ccgaagttcg acgggctctg cagccgctgg tggacctcgc gtcggcgatg
     4861 ggctccgcac tcgtcggcat ctcgcatttc agcaagggtg ggcagggaca agacccagca
     4921 tcgcgggtcg tcggctcggt agcgttcaca gccgtcgcgc gggttgtgtt ggtcgcagcc
     4981 aaagtgaagg gtgacgacgg acaagaccgt cgtatcctgg cccgggggaa gtcgaacatc
     5041 gggcctgacg atggcggatt cgagtacgcc atcagccagg tcgaagcgct tccgggtatc
     5101 cgggctgcac gcatcgaatg gggctcgtcg gtagaaggga cggcccgaga actgctgacc
     5161 gacccggccg aggaaggcga agagaacaac gctctggact ctgcggctgc gttcttgagg
     5221 gaggtgctcg gagacgactt ggtgcccgtg aaaagcatcg aggcagaggc caaggacgcc
     5281 ggcattgcct ggcgcaccat tcgccgagcc tccgactcgc tggctgtcgt gaagcgccgc
     5341 ggcatggaaa accggtggta ctggagcctc ccgaagttgt ccaaacccgg caacttgtcc
     5401 aacttgtcca acgtttcgag cttggacaac ttggccaaca tggacacgcc gccggtgggg
     5461 aagagcgccg agaacgacga agggagcgca cgatgagcgt cattgcgctg gtgaccggca
     5521 agctgctgaa ggcgccgaca acgcgctccg gcaggagcgg cgacccgttc actttggcca
     5581 cgatgaaagc cgcgacggat gacggcgacc tcctggtgaa cctcatcggc ttcggcctgg
     5641 cgggcgaaat ccttgcgaag cttggcgttg gcgatgcact cagcgttact gggcgggcaa
     5701 agctgaacca ctggacggcc aatgatggac aggagcggca cggcctgaac gtcgttgtcg
     5761 atggcgtgct caccccgtac caagtgaatg tgcggaaacg ccgcgtgcag ggcggcaaag
     5821 aagaggaggc ggagtcatga cccgtggagc aacgcccaag gcgaagcgaa agccatcggc
     5881 agaggtcgag aagacgccgc aacggcgcac ggcggtcgtc gtggaatgcc gcagcgtcgc
     5941 cgacaactac ccggcggtgg cgcgcactct gtctcggcct gaagtgagcg ccgcggcaac
     6001 catcgagaaa tggcaaccag acacccacga cgtgaacgac cttgcgtccg aactggcagc
     6061 ccaggtgtcg gcagtcaact cgggcgacct tggccgggcc gaggcgatgc tcatcgccca
     6121 ggcgcacacg ctgaacgagg tattcaacaa cctgctgcgg cgcgcgatga accagacagg
     6181 actgccacag tgggaggcct acatgcgcat ggggatgaag gcgcagagtc agtgccgcac
     6241 cacgctcgag gccctcgcgg agattaagaa cccgagagcg gttgccttcg tgaggcaggc
     6301 caacattgcc catggccccc agcaagtgaa taacgcggcg ccaacagcct cgcccgacca
     6361 gtacatgcag gcgggttcgg gcgcgcagaa acaagagaac gagccaagca aactattgga
     6421 gcaacagcat ggccagtggc tggacgccgg agcgcagagc tcggcaagcg gagctgataa
     6481 ggcggtggcg cccttgggaa cacgcgacgg gtcccgcatc ggacgcaggt aaggcaaagg
     6541 tctcgcgcaa tgcctggaag ggcggaacca gacaggcatt ccgtgacctc gccaacgcgc
     6601 tgaggcaaca gcgccgattg ctggacgaac ttctcgacgc gccgcctgcc gactcagaag
     6661 cctagccgcg aattacgcgc gcacgcacgc gcgatgcgtg cggcagacgg gcttgtacct
     6721 tcgacctgac accaaactgc tggcggttgc agatgctggt cttcacgggc tgaggtggga
     6781 gacaacgtag tcaaccagag ccaggagcgc atatgcgccc agcaccgcga cgaatggcaa
     6841 ccatcggtgc ttcctggcga tggatgggtc gaagagcacc ccgagccatt tgctccgcga
     6901 gtaccaactt gcgagccgcg ctcgttcggc ctgctgcgtc gcggcctcgc gctcctgcat
     6961 cttgagccgg tgcatgacgt tgccgcgggc ggcgtcctca atcgaaggct ggggcgtctt
     7021 gctgccggcg ccgaaggcca cggccagcgc gctcggtgcc agcattgccg cgagcgccag
     7081 tcccaaaacc agcgtcaaca agtcgcggat ggtcagggcc gcgaacggct tgtctagtgc
     7141 tggccaaccc catgcggcgg cttcgtaggc gccccagagc aggccaccac cgactgccaa
     7201 ccgaaccaga cgaaggaagg cgctttcgac ggcggccatc gcatgggttg ccctcttgcg
     7261 ccatgcagga agcgctcgca gccgagcggc ttcctgccga accaaggaat cgacgtctac
     7321 gttctcagtg gtcatcaacg cccccggccc tcaaacactc ggcacagtat gtcgccgtga
     7381 gacggaatag ctcaaccgct ggtctgctcg ggggagatag tctccgcctg atgcacctat
     7441 gaagaaagac caccgctggc gccacaccca tcgtggcaac catcatgacg cctgcctgac
     7501 gtgcgaggcg caccgaaccc gcagagctgc cgggccacgt tggccagcca gctaaaaggt
     7561 tcttcgggaa gggaaaggcg cagagcgcgc caaaacctaa agccacccgg tggaatcgac
     7621 acttcacgcg acttcagtac atcgcacgtc gattgatggg tttcatgatg ggtgcggccg
     7681 gaaaaccggc cgcaaacccg catgaacact agctttttgg cggagagggt gggattcgaa
     7741 cccacggtac cttgcggtac gcctgatttc gagtcaggta caatcgacca ctctgccacc
     7801 tctccaggat tcttggcgga tcgagcggcg ggagccggtt cgatcgttcc gcgtggtcaa
     7861 ttcagcggag tccgtgactc tagcacagga actcggtggc ggcaagtctc aggcgttcgg
     7921 ggcgtcgtcg caactcagcc gccgcccatg ccgaagcgca ggaaggtcac gcggaccttg
     7981 ccgtcgacga cgcgcgcgtc gcgcaccgcc agcttggcga gcgcggcctg cggagagtcg
     8041 gcgcggatgg tgtagatcgg gtggccctcg agcgcgcgcc gcagcacgga cgggccgcgc
     8101 tccttgacga cctcgacgag ttcggacggg aagccgacca gctggaagct gtcgatgcgc
     8161 acgtcgtcga ggtagaactc gccccggtag cgcacgtagc gcagcgtgcc cgagaacgcg
     8221 accttgccgg aggccgtgcg atgacccagc gagacgagga cgtcggcggc caggccgatg
     8281 cgatcggagc cttcggtgag cgtcaccttg gggtcgctga gggtcaggca ggcgaccatc
     8341 agcgcgcagt tgcgctgcgg gaagcgggcg tccagccgcg cctggagctg cgcctcgtcg
     8401 atgtccagcg tcgcctcggg cacgaacgcg acgatcgccc accaggcgag cgcgcccgcc
     8461 gcaagcgccc cgccgacggc catcgccgcg cgccggccgt tcatggcgcc gaggctccgg
     8521 gcggcggcgg ggggcggcgg ttgcggcgca caccgtacat cacgcccagc atcacgcaaa
     8581 cggcctcgca ggcgatgtcc agcagctgca ggtcgagcgg gccgtcgccg ccggtgatca
     8641 ggcaggacaa caccaccatg atcgcgccga agcggaactc gtcgcgcatc acgacgcgcg
     8701 cgcaggcgta gccgccggcc acggagcacg cccagccggc cagcatctcg atcacgccga
     8761 cgacggagct agggccgagg tcgtccaggg ttcgccgcac ctcgtcgtcg gacagccccg
     8821 catgcatcag cccgagctgg tagatccctc gcagcgccag gctcgcgacc gtcgagccca
     8881 cgatgtcgat gaccaggccg accacgatcg cctgcaccgg cgtgcccgcg acatcgccct
     8941 ggacgggcgg cttgcccttg agcgggctgt cgggcggcgg gttggtcggc gacgtgggca
     9001 tggacggatg gcgtggcccg ggcgggcggg gaagtcgtat tttggcggaa gcgcccggcg
     9061 gctctttcat ggagcggtcg ggccggctct catgcatcgg gagggtcgtc gccgcgcatc
     9121 agctgcagca gctcgcgcgc cagcggcacg gcggcgcgcg ggtcgccgtc ggcctcgagc
     9181 atgccctcgg cgagctcgcg cttggcctgg tgcaggtcga ggatgcgttc ctccagggtg
     9241 ccggcatgca ccatgcggta gacggttacg ggacggcgct ggccgatccg gtgggcgcgg
     9301 ccgctcgcct ggtcctcggc ggcagggttc caccacgggt cggcgatcac gacatagtcg
     9361 gcgaccgtca ggttcaggcc gaagccgccc gccttcaggc tgatcaggaa caggtcgccc
     9421 tcgccggcct ggaaggcctc gacgcggcgg gtgcgctcgg ctgagggcgt gctgccgtcc
     9481 agcgtctggt aggcgatgcc cgcggcgtcg agcggctcgc gcagcagcgc gaggaaatcg
     9541 acgaactggc tgaagaccag cgccttgtgg ccgttggcga cgagctccgc cgccagctcc
     9601 gcgaacgccg ccaccttcgc gcccgggcgc agccccggcg tcacgagccg cggatcgcat
     9661 gccgcgcggc gcaggcgcgt caggccggcg aggatgttga ggtgcgcctg cccggcgccg
     9721 tcgcccgaca ggctcttctc cgcggcgacc agcgcctcgc gccgcagcgc ctcgtagtgc
     9781 gcctgctcgg cctcgtcgcc ctcgacgcgc aggacgagct ccgtgcgcgg cggcagctcg
     9841 gcgagcacct gcgccttcgt gcgccgcagg acgaagggcg cgatcaggcg cttgagcgtg
     9901 cgctgggcgg cgcgatcgtg ctggcgctcg atgaggttgg cgaagcgctc gttgaagcgc
     9961 gcctgcgaac cgagcagccc cggattgcac gcgcgcatga ccgaccacag ctcgccgagc
    10021 cggttctcga tgggcgtgcc cgacatcgcg agccggaacg ccgcctgcag cgcgaagacc
    10081 gcctggctgc gcttggcggc ggcgttcttg atcgcctgcg cctcgtcgag caccagcgtg
    10141 gcccacggct tcgcggcgaa ggcggcctcg tcgagctgca gcatcgggta cgagaccagc
    10201 agcacgtcgc gccggcccag gcggccgatc gcgttcgcgc gctcgcccgc ggcgccctgt
    10261 tccgcataga cgtgcacccg cagcgtcggc gcgaagcgcg cgcattcgct gcgccagttg
    10321 cccacgagcg aggtcggcat caccaccagc gccggcccgt cggcggcgcg cgcgagcagc
    10381 acggcgatcg cctgcagcgt cttgcccagc cccatgtcgt cggccaggca ggcgcccagg
    10441 ccggcggcgg cgagccgcat cgcccactcg aagccctcct cctggtacgg ccgcaactcg
    10501 gcctgcagcg ccccgggcag cttcggcacg atccggcgcg cctcgtcgag cgccatcagc
    10561 cgcgcggcgt acgcggcgtc gtgctcgacg tgcagtccct cggacgccgc ctgcagccag
    10621 ggcgcggcga cggtcggcag ccgcatctcc tcgaccgagt ccagccgcgc ggcctgggcc
    10681 tcgacgacgg cggcgatgtc ggcgacgcgc tcgcgcagcg cctgcgacag cgcgaggtag
    10741 cggccgtcgc cgagcggcac gaagcggctg cgctgggcca cggcgaagcc gatcagctgc
    10801 tgcaggctca cgaccagccg ctcgtcgacc ttcagctcgc cctgcaggcc cagccagtcc
    10861 tcgtgcgtgc gcaccttcag cacgaggttg tccagcgcgc cgggcagcac gcgcaccggg
    10921 cggccctgcg gccagtcgag cgccgcgatc gcgtgatgcg gctgcagccg ttcgagcagc
    10981 gcgagggcgt cgtccgcggc gtcgagctgc cattcgcagg gcgcgtcggg cgcgagcggc
    11041 accagcatcg ggcaggcgtc gaggatcgag tcgagatgct cgcgctcgcg ctccaggtcg
    11101 cgctgcacgc ccaggcgctc gccgtccagc gtcgcgacga cgcgcgcgcg cccgcggccg
    11161 gggatcagcc gc
//
LOCUS       DASVLN010000341         5376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_179197, whole genome shotgun sequence.
ACCESSION   DASVLN010000341 DASVLN010000000
VERSION     DASVLN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179197"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VF453_08420"
     CDS             complement(<1..373)
                     /locus_tag="VF453_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7687716.1"
                     /translation="MRVFVTGATGWVGSAVVQELLSAGHEVLGLARTEEGAAALAATG
                     AQVRRGTIEELDLLREAAASADGVVHCAFNHDFSRFAANCEADRRAIDALADGLAGSA
                     RPLLVTGGLAYLAPGRPATEDD"
     gene            469..1329
                     /locus_tag="VF453_08425"
     CDS             469..1329
                     /locus_tag="VF453_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7687717.1"
                     /translation="MPSPPASADAGNALGAYLKDRRTRLDPHALGYSTGRRRTPGLRR
                     EEVAQRAHISATWYTWLEQGRGGAPSAEVLERIARALLLTDVEREHLFLLGLGRPPEV
                     RYPGDVHGVTPRLQRVLDALDVCPAFVKTPLWDVVAWNRAAACVFTDYAALPPEQRNV
                     LRLVFREQRVRRANADWLGVARFVVAAFRVDVARAGAADQAAALVEDLRRTSPEFDAI
                     WRDNDVRHHGEGAKQLRHPVAGLLAFEYSAFAVDGRPDLGMVVYNPVTARDAQRVRAL
                     MAAESEAVDA"
     gene            complement(1351..1734)
                     /gene="crcB"
                     /locus_tag="VF453_08430"
     CDS             complement(1351..1734)
                     /gene="crcB"
                     /locus_tag="VF453_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX7687718.1"
                     /translation="MQSLILVFVGGGLGAALRHLVNLGSLKVVGTDWPFGTFFINVSG
                     SLLMGIVAGLFALKLSLPPSLRLFVATGILGGYTTFSTFSLETALLYQRGQPGAAALY
                     AVGSLAAGVLALFAGMALVRALVAG"
     gene            complement(1775..2539)
                     /locus_tag="VF453_08435"
     CDS             complement(1775..2539)
                     /locus_tag="VF453_08435"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: protein motif:HMM:NF014650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HEX7687719.1"
                     /translation="MRRSQVIAAVAASVVIAAGFGVAWLHHGRSALWRIVSEDCVPAA
                     AAGRSSKCAAVTRDPSAPSDFVVLKDRRGALQYLVLPARRVTGIEDPALLAPEAGDYL
                     ADAWRERRWMDVSRGAPVPREDVALALNSAWSRSQDQLHVHVSCVRADLRARLQALDA
                     SIGTAWTPLPGGWRGHPYLVRRIVADTLDGTGLVRELGRAHPGEMGRQAIAVVGARWQ
                     GRPSFWVLETHVDLADRWLGGVEGDVQDHACAVLAA"
     gene            2777..4408
                     /locus_tag="VF453_08440"
     CDS             2777..4408
                     /locus_tag="VF453_08440"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX7687720.1"
                     /translation="MSFARSPRRALRRLMTVLRPTLFSTAACAATAALAAPAAVCTAP
                     IAAVNTSAAVANVGTGTAASCTEAALRAAVLSASTIKFDCGAAPATIAITSQIDIPTD
                     RNIVIDGGGKVTLDGGGRTRILSVDRENFRTSRIGLTLQHITLANGKAAGTRYVAPNA
                     GNASCAYGWADGGGGAVYVRDSVLHAIDVTFRKNAAETPGPDVGGGAIYAVASLDVTV
                     VGSTFDGNSGANGGAVGLLQSDGRFVNDLFQNNAASGTGANFVGGAAAGCPGVAQANQ
                     GGAGGNGGALAIDGGSDGAQLVCGSTFTANAAGEFGGALFRTPDGTPRQTTIDRTLMQ
                     TNRAKKGGALYVQNSKPLVITASTLAGNTAVSAGAADLIGDRLNVINSTFANNTATLG
                     VGGALVVSGSDASGFIRNATFSGNQSTGGPGYFSAAIFGTLAFPVTNTVFANNLSKDA
                     GSPMQCGFAAGSGSADMQWPQKRPVGGLADTACVSGIRFVDPQLGAIGSHGGPTPTVA
                     PATTSPLRNAGTNCPATDQRGVARNTAHCTIGAVE"
     gene            complement(4444..5112)
                     /locus_tag="VF453_08445"
     CDS             complement(4444..5112)
                     /locus_tag="VF453_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652759.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX7687721.1"
                     /translation="MPRSSLDIVPLGALREAALHCRECPLGARATQTVWGEGSARAPL
                     MLVGEQPGDREDLVGRPFVGPAGRLLDRALQALGWRREELYVTNAVKHFKYELRGKRR
                     MHKTPGQLEILACHHWLESEIAAVRPAGIVALGATAARAVLGHPARVLAERGTWQRRE
                     DGVRVLVTLHPSALLRGDPGEREAAFAAWLRDLEHASELLPARVDGAPRSAAPGHGRG
                     AAAR"
BASE COUNT          753 a         2050 c         1918 g          655 t
ORIGIN      
        1 ggtcgtcctc ggtcgccggg cggcccggcg caaggtaggc gaggccgccg gtcacgagca
       61 gcggacgcgc gctgcccgcg aggccgtcgg ccagcgcgtc gatcgcgcgc cggtcggcct
      121 cgcagttcgc ggcgaagcgc gagaagtcgt ggttgaacgc gcagtggacg acgccgtcgg
      181 ccgacgccgc ggcctcgcgc agcaggtcga gctcctcgat cgtgccgcgg cggacctggg
      241 cgccggtcgc ggcgagcgcg gcggcgccct cttcggtgcg ggcgaggccg agcacctcgt
      301 gccctgcgga cagcagttcc tggacgacgg ccgagccgac ccagccggtg gcgccggtga
      361 cgaagacacg catgggaaat ccttcgttgg ttgacgatgg acgccagtgt cggtagacgc
      421 tttatccggg taaagtggtg actttataca ggtatgagaa ctgccaggat gccgtcaccc
      481 cccgcctccg ccgacgccgg caacgcgctc ggcgcctacc tcaaggatcg gcgcacgcgc
      541 ctcgacccgc acgccctcgg ctactccacc ggccgccgcc gcacgcccgg cctgcggcgc
      601 gaggaggtcg cccagcgcgc ccacatcagc gcgacctggt atacgtggct cgagcagggc
      661 cgcggcggcg cgccgtcggc cgaggtgctc gagcgcatcg cccgcgcgct gctgctgacc
      721 gatgtcgagc gcgagcacct gttcctgctc gggctgggcc ggccgcccga ggtgcgctat
      781 cccggcgacg tgcacggcgt cacgccgcgc ctgcagcgcg tgctcgacgc gctcgacgtc
      841 tgtcccgcct tcgtgaagac gccgctgtgg gacgtcgtcg cctggaaccg cgccgccgcc
      901 tgcgtgttca ccgactacgc cgcgctgccg cccgagcagc gcaacgtcct gcggctggtc
      961 tttcgcgagc agcgcgtgcg gcgcgcgaac gccgactggc tcggcgtcgc ccgcttcgtg
     1021 gtggccgcct tccgcgtgga cgtcgcgcgt gccggcgcgg cggaccaggc cgccgcgctc
     1081 gtcgaggacc tgcgccgcac gagccccgag ttcgacgcga tctggcgcga caacgacgtc
     1141 cgccaccacg gcgagggcgc caagcagctg cgccatccgg tggccggcct gctcgcgttc
     1201 gagtactcgg ccttcgccgt cgacggccgg cccgacctgg gcatggtcgt ctacaacccg
     1261 gtcaccgcgc gcgacgccca gcgcgtgcgg gcgctgatgg cggccgagtc ggaggccgtc
     1321 gacgcctagt gcgacgccag gacggcgcgt tcagccggcg acgagcgccc gcaccagcgc
     1381 catgcccgcg aacagcgcca gcacgccggc cgccagcgac ccgacggcgt acagcgcggc
     1441 cgcgccgggc tggccgcgct ggtacagcag ggccgtctcc agcgagaagg tcgagaaggt
     1501 cgtgtagccg ccgaggatgc cggtcgcgac gaacagccgc agcgacggcg gcagcgacag
     1561 cttcagcgcg aacagccccg cgacgatccc catcagcagc gagccgctga cgttgatgaa
     1621 gaaggtgccg aagggccagt ccgtgcccac caccttcagc gagcccaggt tgacgaggtg
     1681 gcgcagcgcg gcgccgaggc cgccgccgac gaagacgagg atcagtgatt gcatcgaagg
     1741 tgctcctggg gatcgaggga atcgggcgcg ggcgtcaggc cgcgagcacg gcgcacgcgt
     1801 ggtcctggac gtcgccctcg accccgccga gccagcggtc cgcgaggtcg acgtgcgtct
     1861 ccagcaccca gaacgacggc cgcccctgcc agcgcgcgcc cacgacggcg atcgcctggc
     1921 gccccatctc gcccggatgc gcgcgcccca gctcgcgcac gagtccggta ccgtccaggg
     1981 tatcggcgac gatgcgccgc acgaggtagg gatggccgcg ccagccgcca ggcagcggcg
     2041 tccaggcggt gccgatcgag gcgtcgagcg cctgcaggcg ggcccgcagg tcggcgcgca
     2101 cgcacgagac gtgcacgtgc agctggtcct ggctgcgcga ccaggcggag ttcagcgcca
     2161 gcgccacgtc ctcgcgcggc accggcgcgc cgcgcgagac gtccatccag cggcgctcgc
     2221 gccacgcgtc ggcgagatag tcgcccgcct cgggcgcgag caacgcagga tcctcgatgc
     2281 cggtcacgcg gcgcgcgggc aacaccaggt actgcagcgc gccgcgccgg tccttgagga
     2341 cgacgaagtc gctcggcgcc gacggatcgc gcgtgacggc cgcgcacttc gacgaccggc
     2401 ccgcggccgc tgcgggcacg cagtcctcgc tgacgatgcg ccacagcgcc gaccgcccat
     2461 ggtgcagcca cgcgacgccg aagccggcgg cgatgacgac ggacgcggcg acggcggcga
     2521 tgacttggga acgtctcatg gcgatggagc gcggcgtggg ccggacggcg cgcgattatc
     2581 gcagcgcgac cgtcgacgcc gccctggcga ggccgccacg cggccgtggc gcaacggatg
     2641 caagctacgg ttgcacatgg tcgcggatcg cggagcctta ggttacctgg ccaacattcg
     2701 cgcgcactcc gcgacagaca ccggcgcgcg gattgccgat agtggggttt tgcttcgttt
     2761 ccgtcaggct ttccccgtgt ccttcgcccg ttccccacgg cgcgcgctgc gccgcctcat
     2821 gaccgtgctg cggcccaccc tgttctccac cgcggcctgc gcagccaccg ccgcgctcgc
     2881 ggctcctgcc gcggtctgca cggcgcccat cgcagccgtc aacaccagcg cggccgtcgc
     2941 caacgtcggc accggcaccg cggccagctg caccgaggcc gcgctgcgcg ccgccgtgct
     3001 gtccgcgtcg acgatcaagt tcgattgcgg cgccgcgccg gcgaccatcg cgatcacctc
     3061 gcagatcgac atccccaccg accgcaacat cgtcatcgac ggcggcggca aggtcaccct
     3121 ggacggcggc gggcgcaccc gcatcctcag cgtcgaccgc gagaacttcc gcaccagccg
     3181 catcggcctg accctgcagc acatcacgct cgccaacggc aaggccgcgg gcacgcgcta
     3241 tgtcgcgccg aacgcgggca acgcgtcatg cgcctacggc tgggcggatg gcggcggcgg
     3301 cgcggtctac gtgcgcgatt cggtgctgca tgcgatcgac gtcaccttcc gcaagaacgc
     3361 cgccgagacg ccgggacccg acgtcggcgg cggcgcgatc tacgccgtcg cctcgctgga
     3421 cgtgacggtc gtcggctcga ccttcgacgg caacagcggc gccaacggcg gcgcggtggg
     3481 cctgctccag agcgacggca ggttcgtcaa cgacctgttc cagaacaacg ccgccagcgg
     3541 caccggcgcg aacttcgtcg gcggcgcggc cgcgggctgc ccgggcgtcg cgcaggccaa
     3601 ccagggcggc gccggcggca acggcggcgc gctggccatc gacggcggct ccgacggcgc
     3661 gcagctcgtg tgcggctcga ccttcaccgc caacgcggcc ggcgagttcg gcggcgcgct
     3721 gttccgcacg ccggacggca cgccgcgcca gacgaccatc gaccgcacgc tgatgcagac
     3781 caaccgggcg aagaagggcg gcgcgctgta cgtccagaac tcgaagccgc tcgtgatcac
     3841 cgcctcgacg ctggccggca acacggccgt ctcggccggt gccgccgacc tcatcggcga
     3901 caggctgaac gtgatcaact cgaccttcgc gaacaacacg gcgacgctag gcgtcggggg
     3961 cgcgctggtc gtgagcggca gcgacgcgag cggcttcatc cgcaacgcca cgttctcggg
     4021 caaccagtcg acgggcggcc cgggctactt cagcgcagcc atcttcggca cgctcgcctt
     4081 cccggtcacg aacaccgtct tcgccaacaa cctgagcaag gacgcgggct cgccgatgca
     4141 gtgcggcttc gcggccggca gcggaagcgc cgacatgcag tggccgcaga aacgcccggt
     4201 cggcggactc gcggacaccg cctgcgtgtc gggcatccgc ttcgtcgatc cgcagctcgg
     4261 cgcgatcggc agccacggcg gcccgacgcc caccgtcgcg ccggcgacga cgagcccgct
     4321 gcgcaacgcc ggcaccaact gcccggcgac cgaccagcgc ggcgtcgcgc gcaacaccgc
     4381 ccactgcacc atcggggcag tcgagtaggt cggatccatc gatacgaccg cgggtatcgc
     4441 ggatcagcgc gccgcagcgc cgcggccgtg tcccggcgcg gcgctccgcg gcgccccgtc
     4501 gacccgcgca ggcagcagct cgctcgcatg ctcgaggtcc cgcagccacg ccgcgaaggc
     4561 ggcctcgcgc tccccgggat cgccgcgcag cagcgccgac gggtgcagcg tcaccagcac
     4621 gcggacgccg tcctcgcgcc gctgccaggt accgcgctcg gcgagcacgc gggccggatg
     4681 gcccagcacc gcccgcgcgg cggtcgcccc cagcgccacg atgccggccg ggcgcacggc
     4741 cgcgatctcg ctctcgagcc agtggtggca ggcgaggatc tccagctgcc ccggcgtctt
     4801 gtgcatgcgg cgcttgccgc gcagctcgta cttgaaatgc ttgacggcat tggtgacgta
     4861 gagctcctcg cggcgccagc ccagggcctg cagcgcccgg tcgagcaatc gccccgcggg
     4921 cccgacgaag gggcggccga cgagatcctc gcgatcgccc ggctgctcgc ccaccagcat
     4981 cagcggcgcg cgggccgatc cctcgcccca gaccgtctgc gtggcgcgcg cgccgagcgg
     5041 gcattcgcgg caatgcagcg ccgcttcgcg cagcgcgccc agcgggacga tgtcgagcga
     5101 tgagcggggc atggcgttct ccggtatggc gaggtcggac ggccgcattg cggcaacgtt
     5161 cgtgccggcg cgggcccact aagatcgcgg ctcccgtcat cgcccgcacc gctcgttcgt
     5221 gatcccgctc ccgttccatg ccgcctcggt cgcggccgtg ctcgcgctcg gcggggcgtt
     5281 cgcggcgttc gcggccatgc gcgcgtccgg gcgtcgcgcg cggcgggcct ccgatgcgga
     5341 cgcggtcgcc gcgaaggccg catcgtcgga cgtcgt
//
LOCUS       DASVLN010000342        12497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_231947, whole genome shotgun sequence.
ACCESSION   DASVLN010000342 DASVLN010000000
VERSION     DASVLN010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12497
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231947"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..753
                     /locus_tag="VF453_08450"
     CDS             202..753
                     /locus_tag="VF453_08450"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7687722.1"
                     /translation="MPTVLSCGVVLLDSSRRLFACHATGTARWDLPKGLSDPAEPPRA
                     AAIREAWEEAGLRIDPDLLADLGEFAYLPGKRLHLFAARVASDAFDLADCRCRSFFAH
                     HRTGRATPEADAYAWKPLADAPGWAGKGLVKVLGRIDWARLDALPEVGRVEVDTSASS
                     AAATIAAAGIDATAPAATRGDRR"
     gene            750..2297
                     /locus_tag="VF453_08455"
     CDS             750..2297
                     /locus_tag="VF453_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7687723.1"
                     /translation="MTLAMSWDEWAAHDALGLAQRVRDGEVTPAELASQAAAAIGRVN
                     GALDAVVEVFDDVVADPLADGMRPDGAFAGVPYLMKDLGPTLAGRLQEFGSMLMQGHR
                     PREDSFLTAQIRRAGLNIVGRTTTPEFGVCSACENRLHVTRNPWDTAFTTNGSSAGTA
                     AVVAAGVLPMAHATDGGGSIRIPAGATGNLGLKVSRGVFSLAPGLSDLSGLVSIQGCH
                     SRTVRDTAAFVDACRGGAPGEFMPFWRAEVPYLELIRSDPRPLRIALSHEWGDYRATP
                     HFVAELERAGRLLEGLGHHVDWRVPPLDFRAAFDAQTTCYISNFAQVIAGHLARLGLS
                     APPPDRLEPINIGIWKAGLHLSYGERAAMQAVFNATSRAFGAFMEDWDIVLTPVTALP
                     TPAVGATDYLTSSTNPSVHDWFAHLWRNFAYTPIANLCGTPAISMPLGAQESGLPLGI
                     QALTRQGDDGLLLQLAAQVERALDGAWNGGRRAGAHVAAQAVGDKEPEVRRDACRRQP
                     ASGDPAH"
     gene            2443..7158
                     /locus_tag="VF453_08460"
     CDS             2443..7158
                     /locus_tag="VF453_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7687724.1"
                     /translation="MQVLEEARGISRRGARQLVRAMAVAAAIVSVGSTATAQISVSAN
                     GTPGYSIGVTVPPGIGGMVPNLALNYTGGSVNGPVGYGWSLSGLSMITRCAPTVAADG
                     VAGGVRYAPSDRLCLDGQRLIETDANGIPVASTPAANGALLDANGHSDSGYTEYRTQT
                     DLYARIRAYGMANGTTANGPAYFKVWTKAGQIYEYGAGPSSSSSTNALISPYGSSIAS
                     AWAVARISDTVGNFIDFKYSYSNTAWGSGPNAGSPTIGHEWELAEIQYTGTQNANVSQ
                     AANNKIVFEYESRPDTPYSAQDRSEAYHLGFKNVSVQRLKAIRTYVNWPSTSLGVVMP
                     SGALAPVPATAVEVRVLKLGYETGPVTHRSRLISEQDCFGTSESTCLPPTTISYATNP
                     TNETYAPNSTFAASSLATLPLTNAAGTLGVLLGDFNGDGRTDIIRWSDTPSQNLLFTS
                     NGDGTFTQNTAGVAGLTLFRSDGCYSSIAMDFNGDGLTDILRVSQGTATSASGVTTTC
                     PIPIVNILYLSKGDGTFTPITLPSNISLAQVVAQPKATPTCPSTQTIGVPKTTSMMQG
                     AAVVDGAAKPQTTCAKVITIYSSNGGSNFHIMDVNGDGIPDIVTTITPAYQDQLTQPN
                     PDTLCGICTHVYLGSTAGTFSELTTTNIANHTVYGPPSGRYAPLRFHPYVVDMDGDGL
                     SDLLVDSGIWRSLGNGNFQQLTTSLSGSCQYPMDFNGDGRVDCMNLRAPIASNWLGLS
                     DGSGNFPSVANFNLTGAGVLTPTAIPTPVGSGVGVVFADLDGDGRTDIIRWSDDPTQN
                     AAYLSNGDGSFRQSTAFNLNTAAYALHKSDLSVDFVTGDFTGNGTTEILRTVSSITAG
                     SPATTNQLFVKNSTIPSEELKSVTPPSGLTSTVTLFSLPNSSSALGPRYTSARSLTNP
                     LPYPKVDLTSPIRVVETVETATGVGTTTTKSEYAYGGFRAALDGRGALGFLTVSEQHS
                     APDGSPLTTTTTYLQDGSYQGMAAVSQTSDAALNVGAPLISRTTNSYCDATSTAAPTA
                     IPSGGTVVAPCPTSSLVQRPYLYQSFEEGWDIDSGRTPLPTVRTSNVFDGFGNPTQIT
                     VVTTGPAVGLSQSVTKTTNSTYLNTTTGDAWLLGRLQTSTQTNTVPNSLASIATSAGS
                     ATYASATTGVAFNATWSAPSFANTMVGQSTTAVATLTNTSSGALPITTPGTSSVAGTD
                     FAFVSTTCGSSLAVGSSCSVTVRFSPTAATTRTGTLTVQTAVGAKSVGLTAQGLTPPA
                     LSLSGCASNSPVTTPASASMTCTVGNTGQTVASGISYGSSLGGMTIGSGPASCGASTS
                     NCGTVTIGSPTSVGNYVGTLSATPSVGSAGTAGFNLTVQSAVVITLTGCSSVTPATPP
                     AVASMTCNVGNSGAAAASGVTYASSAGGMSISGPTSCAANSACGTVRLATPITAGSYT
                     GVLSVTPASGSGASAGFNLYETAAPIPFSGSLTTSSITATGRMGTWTFRNPNAFAVTV
                     TAVGATVTPSYMASDVSIGGTCGSATVAAGGTCTVTLTTVVDCTAYTAMPTVTTAGGA
                     ATGTTALSQPKTTNTRSCT"
     gene            7230..11366
                     /locus_tag="VF453_08465"
     CDS             7230..11366
                     /locus_tag="VF453_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX7687725.1"
                     /translation="MIRSQWGRAGGRALAALLVLGAACVAVRAQSPSNPYSYSRASSF
                     AYDPTSGLLTSETVEPSNPQSCVTTSYGYDNFGNKKSATIANCGAVTGRAGFTTRGSS
                     STFAAATQSVSAGGSAVSATVPAGAFADSGTNALGQTQHSTYDPRFGTVVTTTGPNGL
                     TTTWAYDLLGRKTLETRADGTRTVTAYCYIASRVADTSSNSALCAGLTTATNEVPADA
                     IMFVHSEPRDASTAGVKNGPFTRVYMDRAGRQIRTVTEAFDASTQVGGANRLVVQDVD
                     YNPYGVPAVKTQPRFLDSGSSTSSGSGDVGMSLTVVDRLGRTVASYVADSQGSQPSIT
                     FGGRGTRTASVTSVAYSGLVVTTTNDHGQTKVEEKNVIGEIVRVTDALGAQLARQYDA
                     FGNLVATRDALQNGVAVSYDIRGRKTALSDPDTGVTSYDYDALGELVWQQTANQLAAG
                     TSTTLAYDKLGRVTQRVDPEYTSTWVFDACTMGVGKLCSSSTSTGLTRTYVYDNLGRQ
                     KDVRTDVSGGPSLATTTLYDNLNGRTSGIVYPTGVTVTYGYTAKGFVQSLQTATALVI
                     APRPTTPGGAARSAGTTLAANTALWSALSMNAWGRVEQHRDNVLTGGSAFTTTTGFDP
                     QTGRVTAETAGLGSATSAMNLTYGWDSLDHLVQRADNNGAAWVDPQGHSQSGAVTDSL
                     VYDRIGRLQVDTVAAPGIPNLMRTVEFEYNALGMLLYKSDVGVYSYPASGPGAVRPHA
                     VQSVNGAYVASYSYDADGNAMAASAGAWRQVHYTSFNMPDGQSGIQGPSGGPQYTWLY
                     DENHTRFKEIRTNASVTRTTWLLHPDNQGGLGFEREEQAAGNSNRHFLSVGGTTVGVI
                     VTVDALPTLGSATTPPQLGSVNVVKLEFWHVDRQGSLMATSDHAGAITAIYAYDPFGK
                     RRNRDGEYDASGALVFDWTTNTDNGTPRGYTGHEHLDDVGLIHMNGRLYDPVVSRFMQ
                     GDPFIADPGNLQDYDRYAYCMNTPTTCTDPSGYWHLFGHNILPGIFHNKDIMTVVAIA
                     AAVCLGPESAVWGEWGVLGGLGVDSAVEEAAIAGFVSGAIATGSFKGAVEGAATGAAF
                     SVAGDYINNAGAFSSGGALSDYSTDSAIGQGTFGAVAIHGVVGCVSASAFGGKCGPAA
                     LSAAFSEAASGYAAEASSSYGLVGGVTVSAIVGGTASVLGGGKFSNGAVTAAEGYLFN
                     DCAPGHGGYCRYDLRAALEDSPIAKIWYAAFGAPVEQPDIPNTYVPLSLAATVAPGAG
                     LTVQVGIAVTPGHDLALFGAYGFPRGFDFGAGLAAGYNEGPLSKFQGNSTVVNAGFGI
                     LEAGPGVSYTVTDGKYTGASGSLGFRGVPPVGPTFSVVPVMRTCTLSIIGKTPSGCGD
                     "
     gene            11732..>12497
                     /locus_tag="VF453_08470"
     CDS             11732..>12497
                     /locus_tag="VF453_08470"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HEX7687726.1"
                     /translation="MSVDLMQTSRFARQATFAWRHGSLLRAVVAGASVALIVVAGIAA
                     FQLRNTLLKKQILAARLDRAQRDASMVTTSHEPPPDIVQRLPAAPSVDQLMNVLQRVA
                     AEQGVRVESLQSDDHLATPTELGRLGIAVTLRAPYPAIVHVLQALLDRYPGATLRRLE
                     LARAQAAAATAPMGAMNAMGAAGMPPMPGASVPAVEVEAHVTLDMWSRAVGVVPVPSA
                     QEESPPAAVASEASAAPAARTTAGASAAASAHAASTM"
BASE COUNT         2098 a         4231 c         4140 g         2028 t
ORIGIN      
        1 tacgcgaccc tcgccggccg caagcgggcg atggcgcgcc tcggccagac ccgcagcggg
       61 ctggtcgagg atgcgctggt cgtcgccgcc ggggccgcgg tggtcgcgtg gttcacgggg
      121 ccgaagcgcg catagccggg ccgatcccga acccttgccg caacgcgcgc gatgtcccgc
      181 ctcgcgcggg atactcggcg catgcccacc gtcctgtcct gcggcgtcgt cctgctcgac
      241 tcgtcgcgcc gcctcttcgc ctgccacgcg accggcacgg cgcgctggga cctccccaag
      301 ggcctgtcgg atcccgcgga gccgccacgg gccgcggcga tccgcgaggc ctgggaggaa
      361 gccggactgc ggatcgaccc ggacctgctc gccgacctgg gcgagttcgc atacctgccg
      421 ggcaagcgcc tgcacctgtt cgccgcgcgc gtcgcgtcgg acgcgttcga cctcgccgac
      481 tgccgctgcc gttccttctt cgcgcaccat cgcaccggcc gcgcgacgcc cgaggccgac
      541 gcctatgcct ggaagccgct cgccgatgcg ccgggctggg ccggcaaggg gctggtcaag
      601 gtgctcggcc gcatcgactg ggcgcggctc gacgcgttgc ccgaggtcgg gcgggtggag
      661 gtcgacacat cggcgtcctc cgcggcggcg acaatcgccg cggcaggcat cgacgccacc
      721 gcgccggccg ccacgagggg agaccggcga tgacgctcgc gatgagctgg gacgaatggg
      781 ccgcgcacga cgcgctcggg ctcgcgcagc gcgtgcgcga cggcgaggtg acgccggccg
      841 agctcgcgtc ccaggccgcg gccgcgatcg gacgcgtgaa cggcgcgctg gatgcggtcg
      901 tcgaggtgtt cgacgatgtc gtggccgatc cgctcgcgga cggcatgcgg cccgacggcg
      961 cgttcgcggg cgtgccctac ctgatgaagg atctcgggcc gacgctcgcg ggccgtctgc
     1021 aggagttcgg gtcgatgctg atgcagggcc accggccgcg cgaggacagc ttcctcaccg
     1081 cgcagatccg ccgcgccggc ctgaacatcg tcggccgcac gacgacgccc gagttcggcg
     1141 tctgcagcgc ctgcgagaac cgcctgcacg tcacgcgcaa tccctgggac acggcgttca
     1201 cgacgaacgg ctcgtcggca ggcacggcgg cggtcgtcgc ggcgggcgtg ctgccgatgg
     1261 cgcacgcgac cgatggcggc ggctcgatcc gcatcccggc gggcgcgacg ggcaatctcg
     1321 ggctgaaggt ctcgcgcggc gtgttctcgc tggcgccggg cctgtcggac ctgtccggcc
     1381 tggtgtcgat ccagggttgc cacagccgca cggtgcgcga cacggccgcc ttcgtcgacg
     1441 cctgccgcgg cggggcgccg ggcgagttca tgccgttctg gcgcgcggag gttccctacc
     1501 tcgaactgat ccgcagcgat ccgcggccgc tgcgcatcgc gctgtcgcac gagtggggcg
     1561 actaccgggc gacgccgcat ttcgtcgccg agctggagcg cgcgggccgg ctgctggagg
     1621 gcctcggcca ccacgtcgac tggcgggtcc cgccgctgga cttccgcgcc gcgttcgacg
     1681 cgcagaccac ctgctacatc agcaacttcg cgcaggtcat cgcgggccat ctcgcgcggc
     1741 tcgggctatc cgccccgccg ccggaccggc tcgagccgat caacatcggc atctggaagg
     1801 ccggcctgca cctgtcgtac ggcgagcgcg cggccatgca ggcggtcttc aacgccacct
     1861 cgcgcgcgtt cggcgcattc atggaggact gggacatcgt cctgacgccc gtcaccgcgc
     1921 tgcccacgcc cgcggtcggc gccacggact acctcacctc cagcacgaat ccgtcggtgc
     1981 acgactggtt cgcgcacctg tggcgcaact tcgcctacac gcccatcgcc aacctgtgcg
     2041 gcacgcccgc gatctcgatg ccgctgggcg cgcaggagag cggcctgccg ctgggcatcc
     2101 aggcgctgac ccgccagggc gacgacggcc tgctgctgca gctcgcggcg caggtggagc
     2161 gggcgctgga tggggcgtgg aacggcgggc ggcgggcggg agcgcatgtg gcggcacagg
     2221 ccgtcggcga caaagagccc gaggtgcggc gagatgcttg tcgtagacaa ccggcctcgg
     2281 gtgatccagc ccattaaggc tctcttcagg aattagggtt tgcccgcaat ggcacctgct
     2341 gacttgcgcc gttttgctca ctagactcgt gcttcagaca gtcgacgaac ggcatgacgg
     2401 atcggccgca gccgacgcga gaaacgagtc agggaggcct gcatgcaggt gttggaggag
     2461 gcccgcggca tcagccgtcg gggcgcgcgg cagctcgtgc gcgcgatggc agtggcggca
     2521 gcgatcgtct cggtggggtc gacggccacg gcgcagatct cagtctcggc gaacggcaca
     2581 cctggctact cgatcggcgt gacggtgccg cccggcattg gtggcatggt gccaaatttg
     2641 gcactcaact acaccggtgg cagcgtcaat gggcccgtcg gttacggttg gtcgctttcc
     2701 gggctgtcga tgatcacccg ttgcgcaccg accgtggccg cggatggcgt tgctggcggc
     2761 gtgcgctatg cgccttctga ccgcctgtgc ctggatggac aacgcctgat cgagacggat
     2821 gccaatggca tacctgttgc ttcgacgcct gcggcgaacg gcgcactcct ggacgccaac
     2881 ggccattcgg actcaggcta caccgagtac cgcacgcaga cggacctgta tgcgcggatt
     2941 cgcgcctacg ggatggccaa cggcacgacc gccaacggcc cggcgtactt caaggtctgg
     3001 acgaaggcag ggcagatcta cgagtacggc gcagggccgt cgtccagctc gtcgaccaat
     3061 gccctgattt cgccttacgg ctcatcgatc gcgagcgcct gggcggttgc gcgcatcagt
     3121 gacacggtcg gcaacttcat cgacttcaag tactcgtaca gcaacaccgc ctggggctcc
     3181 ggcccgaatg caggcagtcc gaccattggg cacgagtggg aactcgccga gatccagtac
     3241 acgggcactc agaacgcaaa cgtctcgcag gcggccaaca acaagatcgt cttcgaatac
     3301 gagagtcggc ccgatacccc gtacagcgcg caggatcgtt ccgaggctta ccacctcgga
     3361 ttcaagaacg tcagcgtgca gcggctcaag gcaatccgca cgtacgtcaa ctggccgtcg
     3421 acctcgctcg gcgtagtcat gccgtcgggg gcgttggctc ccgtaccggc cacagccgtt
     3481 gaagttcggg tactcaagtt gggctacgag acggggcccg tcacgcatcg tagccggctg
     3541 atctccgagc aagactgttt cggcacgagc gagtcgacgt gcttgcctcc cacgaccatc
     3601 tcgtatgcga ccaaccccac gaacgagact tacgcgccta actctacctt cgccgccagt
     3661 agcctggcca cactgccgct gaccaacgcc gcgggcaccc tgggcgtgct gctcggcgat
     3721 ttcaatggtg acggacggac ggacatcatt cgctggtccg acacgccgag ccagaacctt
     3781 cttttcacga gcaacggaga cggcacattc acgcagaaca cggccggggt cgcggggctc
     3841 accctttttc gcagcgacgg gtgctattcg tccatcgcaa tggatttcaa cggcgacggg
     3901 ctgactgaca ttctccgcgt ctcgcagggc acagccacga gtgcctcggg agtgacaacg
     3961 acatgcccga tcccgatcgt caacattctg tacctctcca aaggcgacgg caccttcacg
     4021 ccgatcacgc tgccgtcgaa catcagcctg gcccaggtgg tggcgcaacc caaggcgacg
     4081 ccgacctgtc cgagcaccca gaccattggt gtgcccaaga cgacgtccat gatgcaggga
     4141 gccgctgtgg tggatggcgc agcgaaacca cagaccacgt gcgcgaaggt catcaccatc
     4201 tattccagca atggtggatc gaacttccac atcatggatg tcaacgggga cggcattccc
     4261 gacatcgtga cgacgatcac gccagcctac caggaccagc tcacgcagcc aaatcctgac
     4321 acactgtgtg gcatctgcac gcacgtctac ctggggtcga ccgccgggac attcagcgaa
     4381 ctgaccacga ccaacatcgc gaatcacacg gtttacggcc cgccgtcggg acgctatgca
     4441 ccgctgcgct tccatcctta cgtggtcgac atggatggcg acggcctgtc ggacctgctc
     4501 gtcgactcgg gcatctggcg ctcgctcggc aatggcaatt tccagcagct tacgaccagc
     4561 ctgtccggat cctgtcagta cccgatggac ttcaacggcg atggccgcgt ggactgcatg
     4621 aacctgcgcg cgccgattgc ctcgaattgg ctcggactct cggacggtag cggcaacttc
     4681 ccttcggtcg ccaacttcaa cttgacgggc gcgggtgtgc tgacgccgac cgcgatcccg
     4741 accccggtag gctcgggcgt cggcgtcgtc ttcgccgacc tggatggcga cggccgcacc
     4801 gacatcatcc ggtggtcgga cgatccgacg cagaacgcgg cctacctcag caatggcgac
     4861 ggctcgttcc gacaatcgac ggcattcaac ctgaacaccg ccgcgtatgc gctgcacaag
     4921 agcgacctga gcgtcgactt cgtgaccggc gacttcacgg gcaacggcac gaccgagatc
     4981 ctgcgcacgg tatcctcgat cacggccggt tcgcccgcca ccaccaatca gctcttcgtc
     5041 aagaactcga ccatcccgtc cgaggaactg aaatcggtga cgccgccgtc cggtctgacc
     5101 agtaccgtga cgctcttcag cctgccgaat tcgtccagtg ccctggggcc gcgctatacc
     5161 agcgcgcgtt cgctcaccaa tcccctgccg tatcccaagg tggacctgac atcgccgatc
     5221 cgcgtcgtgg aaacggtgga gacggcgacc ggcgtgggta ccaccacgac gaagagcgag
     5281 tacgcctacg gcggattccg ggcggccctc gacggccgcg gagcgctcgg cttcctgacg
     5341 gtaagcgagc aacattcggc gccggatgga agtcccctga ccacgaccac gacatacctg
     5401 caggatggct cgtaccaggg gatggccgcg gtcagtcaga cgagtgacgc agcgctgaat
     5461 gtcggcgcgc cgctgatcag ccgtacgacg aattcctatt gcgacgccac ctcgaccgcc
     5521 gcgcccacgg ccatcccgag tggtggcacc gtcgtggcgc cgtgcccgac gagctctctc
     5581 gtccagcgac cgtatctgta ccagtccttc gaggaaggct gggacatcga tagtggccgg
     5641 acgcctttgc cgacggtgcg aaccagcaac gtcttcgacg gcttcggcaa tccgacgcag
     5701 atcaccgtgg tgaccaccgg cccggccgtc ggcctctccc agtcggtgac gaagaccacg
     5761 aacagcacct atttgaacac gaccaccggc gatgcatggc tgctcggccg gctgcaaacg
     5821 agcactcaga ccaacaccgt gcccaacagc ctggcgagca tcgcgacctc ggcggggtcg
     5881 gcgacgtatg cgtccgcgac gaccggcgtg gcgttcaatg cgacctggag cgcgccgtcc
     5941 tttgcgaaca ccatggtcgg ccagtccacg accgccgtgg cgacgctgac caacacgtcg
     6001 tcgggcgcac tgccgattac aacgccgggc acgtcgtcgg tggctggcac cgacttcgcg
     6061 ttcgtctcga ccacgtgcgg ttccagcttg gccgtgggaa gttcctgttc ggtgacggta
     6121 cgcttctcgc cgacggccgc gaccacccgc acgggcacgc tgacggtgca gaccgcggta
     6181 ggcgccaaga gcgttggcct gactgcgcag ggattgacac cgcccgcgct gtcgctgtct
     6241 ggatgcgcgt ccaattcgcc cgtcacgacg cccgcgtcgg cgagcatgac ctgcacggtg
     6301 ggcaacactg gccagactgt ggcctctggc atctcctacg ggtccagcct tggcggcatg
     6361 acgattggca gcggtccggc gagctgcggg gcgagcacca gcaattgcgg aaccgtcact
     6421 atcggctccc cgacctccgt gggcaactac gtcggtacgc tttcggcgac gcccagcgtc
     6481 ggcagtgcgg gcaccgctgg cttcaacctc acggttcaga gcgccgtcgt gatcaccttg
     6541 accggctgct cctccgtgac gccggcgacg ccgcccgccg ttgccagcat gacgtgcaac
     6601 gtgggcaatt ccggcgcggc cgcggcgagc ggcgtgacgt atgcgtcgtc ggccggcggc
     6661 atgtcgatca gcgggccgac gagctgtgcg gcgaacagcg cttgcgggac ggtgcgtctg
     6721 gccacgccga tcaccgcggg ctcgtacacc ggcgtgctgt ccgtcacgcc cgcgtccggt
     6781 agcggcgcct cggcgggctt caacctttac gagacggcgg cgcccattcc gttctcgggt
     6841 tcgttgacga cgagttcgat cacggccacg ggccgcatgg gcacgtggac cttcagaaat
     6901 ccgaatgctt tcgccgtgac ggtgaccgcc gtcggcgcca cggtgacgcc gagctacatg
     6961 gcgtccgacg tcagcatcgg cggcacctgt ggctccgcca ctgtcgcggc cggaggcact
     7021 tgcacggtga cgctgacgac ggttgtcgat tgcacggcct acaccgccat gcccacggtg
     7081 acgactgctg gtggcgctgc cacaggtacg accgcgctca gccagcccaa gacaacgaac
     7141 acgcgcagtt gcacctgagg cgttgccggc tcttgcggcg agcggagccg gctgggcgac
     7201 attcaacgaa agaacagcag ggagaagcga tgatccggtc gcaatggggc agggcaggcg
     7261 gacgtgcatt ggcggcgcta ctggtgctcg gcgccgcgtg cgtcgccgtg cgcgcccagt
     7321 cgccgtcgaa tccgtacagc tactcgcgcg cgagcagctt cgcctacgac ccgacttccg
     7381 gcttgctgac gagcgagacg gtcgagccgt cgaacccgca gtcctgcgtg accacgagct
     7441 acggatacga caacttcggc aacaagaaat cggcgaccat cgccaactgc ggggccgtca
     7501 ccggccgcgc agggttcacg actcgcggat cgagcagcac cttcgccgcg gcgacgcagt
     7561 cggtctcggc cggtggctcg gcggtctcgg caaccgtgcc tgccggggcc ttcgcagact
     7621 ccggcactaa tgcactcggc cagacgcagc actcgaccta cgacccgcgc ttcggcaccg
     7681 tcgtgacgac gaccggccct aacggtctga cgacgacctg ggcctacgat ctgctcggtc
     7741 gcaagacgct ggagacgcgc gccgatggca cccgcacggt caccgcgtac tgctacatcg
     7801 ccagccgtgt cgccgatacg agcagcaaca gcgccctttg cgccggcctt accacggcga
     7861 ccaacgaggt gccggctgac gcgatcatgt tcgttcactc ggagccgcgc gacgcgtcga
     7921 ccgccggcgt gaagaacggc cccttcacgc gggtctacat ggaccgcgcg gggcgccaaa
     7981 tccgcacggt gacggaagcg ttcgacgcct cgacgcaggt gggtggcgcg aaccgcctgg
     8041 tcgtgcaaga cgtggactac aacccctacg gcgtgccggc ggtgaagacc cagccccgct
     8101 tcctcgattc cggctcgtcc acctcgtccg gctccgggga tgtcggcatg agcctgaccg
     8161 tggtcgatcg gctggggcgc acggtcgcca gctatgtcgc cgacagccag ggcagccagc
     8221 cgtcgatcac cttcggcggc cgaggcacgc gaacggccag cgtgaccagc gtggcctact
     8281 cgggccttgt cgtgacgacg accaacgatc atgggcagac caaggtcgag gaaaagaacg
     8341 tcatcggcga aatcgtgcgg gtgaccgacg cgctgggtgc gcaattggcg cggcaatacg
     8401 acgccttcgg caacctggtg gccacgcgcg acgcgctgca gaacggcgtg gccgtgagct
     8461 acgacatccg cggccgcaaa acggcactgt ccgaccccga taccggcgtg acgagttacg
     8521 actacgacgc gctgggcgag ctggtgtggc agcagaccgc caaccagctc gcggccggca
     8581 cgtccacgac cctcgcctac gacaagctgg gccgcgtgac gcaacgcgtc gatcccgagt
     8641 acacgagcac ctgggtcttc gacgcatgca cgatgggcgt aggcaagctg tgctcgagct
     8701 cgaccagcac ggggctgacg cgcacctacg tctacgacaa tctcgggcgg cagaaggatg
     8761 tccgaacgga cgtctccggt ggccccagcc tcgcgacgac gaccctctac gacaatctga
     8821 acggccgtac ctccggaatc gtctacccga cgggcgtcac ggtgacctac ggctacaccg
     8881 ccaagggctt cgtgcaaagc ctgcagacgg cgactgcgct ggtgatcgcg ccacgcccga
     8941 ccacgcccgg cggcgcggcg cgcagcgcag gtaccacgct cgctgccaac acggcgctct
     9001 ggtccgcgtt gagcatgaac gcctggggcc gagtcgagca gcatcgcgac aacgtgctga
     9061 ccggcggctc ggcgttcacg acgacgaccg gcttcgaccc gcagacgggc cgcgtcacgg
     9121 ccgagacggc cggcctgggc tcggccacga gcgcgatgaa cctgacctac ggctgggaca
     9181 gcctggatca cctcgtccaa cgcgcggaca acaacggcgc tgcctgggtc gacccgcagg
     9241 gccacagcca atcgggggcg gtcaccgatt cgctggtgta cgaccgcatc ggccggctgc
     9301 aggtcgacac cgtcgcggcg cccggcattc cgaacctgat gcgcacggtg gagttcgagt
     9361 acaacgcgct gggcatgctg ctgtacaaga gtgacgtcgg cgtctacagc tatccggcct
     9421 cgggcccggg tgccgtgcgt ccgcacgcgg tgcaatcggt caacggcgcc tacgttgcga
     9481 gctactcgta cgacgccgac ggcaacgcca tggccgccag cgcgggcgcc tggcgacagg
     9541 tgcactacac gagcttcaac atgcctgacg gccagtccgg catccagggg ccgagcggcg
     9601 gaccgcaata cacgtggctc tatgacgaga accacacgcg gttcaaggag atccgcacaa
     9661 acgcctccgt gacacgcacc acctggctgc tccatccgga caaccagggc ggcctcggct
     9721 tcgagcgtga ggaacaggca gccggaaaca gcaaccgcca cttcctgagc gtggggggca
     9781 cgaccgtggg cgtgatcgtg acggtcgatg cgctgccgac gctcggctcg gcgacgacgc
     9841 cgcctcagct cggctccgtc aacgtggtga agctcgagtt ctggcacgtc gaccgtcagg
     9901 gcagcctcat ggctacgagc gaccacgcgg gcgccatcac ggccatctac gcctacgacc
     9961 cgttcggcaa gcgtcgcaac cgcgacggcg agtacgacgc gtcgggcgcc ctggtcttcg
    10021 attggacgac caacaccgac aacggcacgc cgcgcggcta caccggccac gagcacctcg
    10081 acgacgtcgg cctgatccac atgaacggtc gtctctacga cccggtggtc agccgcttca
    10141 tgcagggcga cccgttcatc gccgatccag gcaacctgca ggactacgac cgctacgcgt
    10201 actgcatgaa cacgccgacg acgtgcaccg acccgagcgg gtactggcac ctcttcggcc
    10261 acaacatcct gccggggatc ttccacaaca aggacatcat gacggtggtg gcgattgctg
    10321 cagctgtctg cctcgggccg gagtcggcag tctggggcga atggggggtg ctcggaggcc
    10381 tgggcgtcga cagcgcggtc gaagaggccg cgatcgccgg attcgtgagc ggagccatcg
    10441 caacgggcag tttcaagggc gccgtggagg gcgcggcaac gggcgccgct ttctcagttg
    10501 ctggcgacta cattaacaac gcgggcgcct tctcgtctgg gggcgcactg tctgactact
    10561 caacggactc cgctattggt caaggaacat tcggagctgt cgcgatacac ggcgtggtcg
    10621 gctgcgtgtc ggcttcggca tttggcggca aatgcgggcc tgcggcgttg agcgcagctt
    10681 ttagcgaagc ggcttctggc tatgcggccg aggcttcgag cagctacggc ctagttggcg
    10741 gagtcaccgt cagtgcgatc gtgggcggaa ctgcatcagt gctcgggggt ggtaagttct
    10801 ccaatggggc agtcacggca gccgaggggt atttgttcaa tgactgcgcc cctggccacg
    10861 gtggctattg cagatatgat ctcagggcag ccttggagga cagtccaatt gccaagattt
    10921 ggtatgccgc cttcggcgca cccgtcgagc aaccggacat tccgaataca tatgtgccgc
    10981 tgagccttgc cgctacggtt gcgccagggg ccgggttgac tgttcaggtc ggaattgctg
    11041 tgacacccgg acacgatttg gcgctgtttg gtgcttatgg ctttccacgt ggatttgatt
    11101 tcggtgcggg tctggctgct ggctataatg aaggccctct gtcgaaattt cagggaaatt
    11161 ctactgtggt aaacgcaggg tttggtattt tggaggccgg gccgggcgta agctacacag
    11221 tcaccgatgg caaatataca ggtgcttccg gttcgctcgg atttagaggg gtcccacctg
    11281 tggggccgac attttcagtt gttccggtga tgagaacatg cactctgtca attattggga
    11341 aaacaccctc cggctgcgga gattgatgtg attttctcta ttcttactag tatcgccctg
    11401 attggtgtgt tgattgcatt agttgcgggt atgctggcaa tcagggattt cttgtgtttg
    11461 atgaggttaa atgctgagct catgcgaggt gcagcgggcc ccgctgaccg tcggaacgtg
    11521 tcaagggatc gcctcgaggc agacgcttct cgccgtcgaa agcgtgcgtt gctttcaatg
    11581 atgtattgct tgttggccat cctgggtagt gctgcaatca cattgatcag aaactatctg
    11641 agcccagcgt gaacgctggg ggcgtgagga acagcatcgc gatagcgtgt tgccgcgctg
    11701 tgcgtccgtt ttcccgcggc aggatgaaaa agtgtcggtg gacttgatgc agacgtctcg
    11761 tttcgctcgc caagccacct tcgcgtggcg ccacggttcc ctgctgcggg ccgtggttgc
    11821 tggcgcgtcg gtggcgctca tcgtcgtagc gggtatcgct gcgttccaac tgcggaacac
    11881 gttgcttaag aagcagattc tggccgcccg gctggatcgg gcgcagcgcg acgcctcgat
    11941 ggtgaccacg tcccacgagc ccccgcccga catcgtccag cgcctccccg ccgccccatc
    12001 cgtcgaccag ctcatgaacg tcctccagcg cgtggccgcc gagcagggcg tgcgcgtcga
    12061 gtcgctgcag tccgacgacc acctcgcgac gccgacggag ctcgggcggc tgggcatcgc
    12121 ggtgacgctt cgagcgccgt atcccgcgat cgtgcatgtg ctgcaggcgc tcctcgaccg
    12181 ctaccccggc gccacgttgc ggcggctcga gctggcgcgc gcccaggcgg cggcagcgac
    12241 ggcgccgatg ggggcgatga atgcgatggg cgcagcgggc atgccgccga tgccgggggc
    12301 gtccgtgccg gccgtggagg tcgaggcgca cgtgacgctg gacatgtggt cgcgggccgt
    12361 cggcgtcgtg ccagtgccgt cggcgcagga ggaatcgccg ccggcggcgg tggcgtccga
    12421 ggcgtccgct gcaccggccg ctcgaacgac tgcaggtgcg tccgctgcag ccagcgcgca
    12481 cgccgcctcc accatgc
//
LOCUS       DASVLN010000343         9326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_18040, whole genome shotgun sequence.
ACCESSION   DASVLN010000343 DASVLN010000000
VERSION     DASVLN010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18040"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /gene="truB"
                     /locus_tag="VF453_08475"
     CDS             <1..851
                     /gene="truB"
                     /locus_tag="VF453_08475"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7687727.1"
                     /translation="AKWLLRAEKAGHTGTLDPLATGLLPLCFGAATKFSQVSLDADKR
                     YVATLRFGVTTTTGDAEGVVVRERAVAFDRAALEAACARWTGPIRQVPPMHSALKHEG
                     KALYEYARAGLEVERAARDVTIHTIAITHWQHADPMTATIDVRCSKGTYVRTLAEDIG
                     ETLGCGAHLAGLRRIASGPLTLEGAVTLAELEAMPEAERERRLLPPDTLLADWPAVHL
                     DAKEAARFLTGLRRRTTEPDRAAVRVYGPGTHALLGSAHIRGGELIADRLLSPAEVQG
                     AADLIT"
     gene            862..2700
                     /gene="typA"
                     /locus_tag="VF453_08480"
     CDS             862..2700
                     /gene="typA"
                     /locus_tag="VF453_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7687728.1"
                     /translation="MTDRTQIRNIAIIAHVDHGKTTLVDQLLRQSGTFAEHEKVVDTV
                     MDNNAIERERGITILAKNCAVSWKGTHINIVDTPGHADFGGEVERALSMVDGVLLLID
                     AQEGPMPQTRFVTKKALALGLKPIVVVNKVDKPGAKPEAVINAAFDLFDKLGANDEQL
                     DFPVVYASGINGWSSLEEGAPGEQWGPDMSALFETVLKHVEPHGGSPDAPLQLQISAL
                     DYSSFVGRIGVGRVNAGTLRPAQDVLVMEGEDGKSFKGRVNQVLKFQGLDRIQAEEAG
                     PGDIVLINGIEDIGIGVTLTDPADPQPLPMLKIDEPTLTMNFCVNTSPLAGREGKFVT
                     SRQIWDRLQKELQSNVALRVKETDEDGIFEVSGRGELHLTILLENMRREGYELAVSKP
                     RVVFRDIDGVRCEPIELVTADVEEQHQGGVMQALGERKGELTNMEPDGRGRVRLEYRI
                     PARGLIGFTNEFLNLTRGSGLISNIFDGYEPHKGEIGGRKNGVLISMDDGEIFTYALG
                     KLDDRGRMFVKAGDPVYEGMIVGIHNRDNDLVVNATRTKQLTNFRVSGKEDAIKITPP
                     IDLNLEYGVEFIDDDELVEITPKSVRLRKRFLKEHDRKRASRENAA"
     gene            complement(2755..3156)
                     /locus_tag="VF453_08485"
     CDS             complement(2755..3156)
                     /locus_tag="VF453_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009769787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX7687729.1"
                     /translation="MEPTALADTYRAYVDCLNARDWARLGDFVDEHAVHNGRALGLAG
                     YRAMLEADVRAIPDLRFEIALLACQPPIVACRLRFDCTPAGELFGLRVDGRRVRFDEH
                     VFYEFAQGRIRQVWSVIDQAAIAAQLASPAP"
     gene            3383..4345
                     /locus_tag="VF453_08490"
     CDS             3383..4345
                     /locus_tag="VF453_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7687730.1"
                     /translation="MGHARAALGMVVVTLLWSTAGVVSRHLQAAESFEVTFFRSLFNL
                     VGLGVALTWMRGASLWTELARAPRVVWMSGVCWAVMFTAFMVALTMTRVANVLVTMSI
                     SPLLTALFARVFLRHRLPPRTWVAIAVAGAGIAWMFGRNMQAADARSLAGMAVAILVP
                     LSTALNYTVLHHAGRAPASAASSARELDAPGADGTATPGEEPDMLAAVLIGAALSALV
                     TLPIAWPFRATPHDLALLFGLGIFQLAIPCLLLVRLTRELASPEVALLGLLEVIFGVL
                     WAWIGVGESPGSSALVGGALVVGALAFNELLSLRARGAARSGAH"
     gene            4406..5533
                     /locus_tag="VF453_08495"
     CDS             4406..5533
                     /locus_tag="VF453_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7687731.1"
                     /translation="MTDPTATTSAAPTVLAERRRHVGVLTLNRPSALNALSLEMIRAL
                     TAQLRAWRDDPEVGAVVLRGANRPGKPPAFCAGGDIRFFHQAARTADPALGDFFTEEY
                     TLNHLIHTYGKPTFVVMDGITMGGGMGLAQGASLRIATEHAKLAMPETLIGLFPDVGG
                     GWFLARLPGRLGEFLGLTGLTLGAGDALAVGWADLCVSADRVDALLQALVDATPADGV
                     GALTAVHAFAADAGAGTLAPHQALIERVFSQPTLAAIVAALQAEEQGDDAEFARATAA
                     LLAKRSPTMLAVTLEQVRRARTMSLADELRMERDLVHHCFHLRPPERSETLEGIRALA
                     VDKDHAPRWQPASIADVDAAEIAAFFESPWTPQAHPLHALA"
     gene            5689..6015
                     /locus_tag="VF453_08500"
     CDS             5689..6015
                     /locus_tag="VF453_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687732.1"
                     /translation="MTQTLPTLLLAALAAVCSAAAPCVASAEDSKDATYEPDKGPNPG
                     LTTDARYHRAKRNADKRLKDSLAQCDRMGAADRAQCIRNANQTHDADVAHADDFLERA
                     PETQER"
     gene            complement(6133..7299)
                     /locus_tag="VF453_08505"
     CDS             complement(6133..7299)
                     /locus_tag="VF453_08505"
                     /inference="COORDINATES: protein motif:HMM:NF038119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHFG family PEP-CTERM protein"
                     /protein_id="HEX7687733.1"
                     /translation="MIVIWVRMLHGHAVSSVDAGGRWGAGVRPLVAARARRRRPQRLI
                     ATSEQTQHTCSSFCRRIDCANVSRRSGQARCPLASSGRARCPSPATNRTDAAGPPTLV
                     RRLPGHLLGGAMAGWARPEREPMGLHLILANALAATAGVAPSPDCSWARPGADPYRDD
                     PVAVLADFALPEDTRARLRAMMQAHRPSDVVRITRDDISGDDGAYEDMRAMHSGHGRV
                     CRGRVERSTWSASHVERALVYCVGDTCVMVPLACHNVALVTRRGKPAKEGRIDIEPAA
                     GPPKVAPTPPASPSDAQDFLPWPPIDGLPPPVGSGGPVDGGSLWGGGPGEPVVGPPPD
                     DDCCVVIGNPPGGEEPVGGPPPPAIPEAPAWTMLLAAWAWLLARRPLPARRRGA"
     gene            7264..8223
                     /locus_tag="VF453_08510"
     CDS             7264..8223
                     /locus_tag="VF453_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7687734.1"
                     /translation="MTVKHPHPDHDHEAGHHPGHSGHAGHDHAAGANERSLKIAFVLT
                     FAFLVVELVGGIVAHSLALLSDAAHMFTDTAVLAIALLAIRISRRPADKKRTFGYHRF
                     EILAAAFNAILLLFVAAYILREAYLRIEAPPEIQSNLMLVIAVVGLLVNLGSMTVLRS
                     GQQESLNVKGAYLEVWADMIGSVGVIIGAGLIKLTGWAWIDSAVAILIGLWVVPRTMT
                     LLKTSVNILLDGVPEDIDIEKVEATLRALPGVRSFHDLHVWSVSSGKATLTVHLVIDP
                     STDGEAEVLPLARERLDRGFGLRHLTIQCETHPCGMADPAGHS"
     gene            8281..9300
                     /gene="dusA"
                     /locus_tag="VF453_08515"
     CDS             8281..9300
                     /gene="dusA"
                     /locus_tag="VF453_08515"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX7687735.1"
                     /translation="MSFESTAYSPWRLSVAPMMDWTDRHCRFFHRLITHGTLLYTEMV
                     TTGALLHGDRPRHLDFSHEEHPVALQLGGSEPEALAACAKLAQDWGYDEVNLNCGCPS
                     ERVQRGAFGACLMREAELVRDSVAAMRDACALPVTVKHRIGVDKVESYEFVRDFVGTV
                     AQGGCMVFIVHARNAWLQGLSPKDNRTIPPLRYEFVYRLKRDFPDFTIVLNGGVKGDD
                     EIARHLEHVDGVMIGREAYHDPWCMADWDARFLGRPAPGLDRAEVEARMVAYLSGLQA
                     AGVPWSQASRHMLGLWNGVKGARRWRQVWSDHKVKGLAPAEVSRLAHEGFEPADGAPT
                     RLAAA"
BASE COUNT         1378 a         3374 c         3299 g         1275 t
ORIGIN      
        1 aggccaagtg gctgctgcgc gcggagaagg ccggccacac cggcaccctc gacccgctgg
       61 ccaccggcct gctgccgctg tgcttcggcg cggcgaccaa gttctcgcag gtcagcctgg
      121 acgccgacaa gcgctacgtc gcgacgctgc gcttcggcgt cacgacgacg accggcgacg
      181 ccgaaggcgt ggtcgtgcgc gaacgcgcgg tcgccttcga ccgcgcggcg ctcgaggccg
      241 cgtgcgcgcg ctggacgggc ccgatccgcc aggtgccgcc gatgcactcg gcgctgaagc
      301 acgagggcaa ggcgctctac gaatacgccc gtgccggcct cgaggtcgag cgcgccgcgc
      361 gcgacgtgac gattcacacg atcgccatca cgcactggca gcatgcggat ccgatgacgg
      421 cgacaatcga cgtccgctgc tcgaagggca cctacgtccg gacgctggcc gaggacatcg
      481 gcgagacgct gggctgcggc gcccacctcg cgggcctgcg gcgcatcgcg agcggcccgc
      541 tgacgctgga aggcgccgtg acgctggccg agctcgaggc gatgcccgag gccgagcgcg
      601 agcgccgcct gctgcccccc gacaccctgc tggccgactg gccggccgtg cacctcgacg
      661 ccaaggaggc cgcgcgtttc ctgaccggcc tgcgccggcg caccaccgag cccgaccgcg
      721 ccgccgtgcg cgtgtacggg cccggcacgc acgccctcct tggcagcgcg cacatccgcg
      781 gcggcgaact catcgccgac cgcctgctca gccccgccga agtgcagggc gctgccgacc
      841 tgatcacttg aagagcctcc catgaccgac cgcacgcaga tccgcaacat cgccatcatc
      901 gcccacgtcg accacggcaa gaccacgctg gtcgaccagt tgctgcgcca gagcggcacc
      961 ttcgccgagc acgagaaggt cgtcgacacc gtgatggaca acaacgccat cgagcgcgag
     1021 cgcggcatca ccatcctcgc caagaactgc gcggtcagct ggaagggcac gcacatcaac
     1081 atcgtcgaca ccccgggcca cgcggacttc ggcggcgagg tcgagcgcgc gctgtcgatg
     1141 gtcgacggcg ttctgctgct gatcgacgcg caggaaggtc cgatgccgca gacccgcttc
     1201 gtcacgaaga aggcgttggc cctcggcctg aagcccatcg tggtggtcaa caaggtcgac
     1261 aagccgggcg ccaagcccga ggccgtgatc aacgccgcgt tcgacctgtt cgacaagctg
     1321 ggcgccaacg acgagcagct cgacttcccc gtggtctacg cgtcgggcat caacggctgg
     1381 tcgtcgctgg aggagggcgc gccgggcgag cagtggggcc ccgacatgtc ggccctgttc
     1441 gagaccgtgc tcaagcacgt cgagccgcac ggcggttcgc cggacgcacc gctgcagctg
     1501 cagatctcgg cgctggacta ctccagcttc gtcgggcgca tcggcgtggg tcgcgtcaat
     1561 gcgggcacgc tgcgtccggc gcaggacgtc ctcgtgatgg agggcgagga cggcaagagc
     1621 ttcaagggcc gcgtcaacca ggtgttgaag ttccaggggc tggaccgcat ccaggccgag
     1681 gaggccggcc cgggcgacat cgtgctgatc aacggcatcg aggacatcgg catcggcgtc
     1741 acgctgaccg acccggccga cccgcagccg ctgcccatgc tcaagatcga cgagccgacg
     1801 ctgacgatga acttctgcgt caacacctcg ccgctggccg gccgcgaggg caagttcgtc
     1861 accagccgcc agatctggga ccgcctgcaa aaggagctcc agagcaacgt cgcgctgcgc
     1921 gtcaaggaga ccgacgagga cggcatcttt gaggtctcgg gccgcggcga actgcacctg
     1981 accatcctgc tggagaacat gcgccgcgag ggctatgagc tcgcggtctc caagccgcgc
     2041 gtcgtcttcc gcgacatcga cggcgtgcgc tgcgagccga tcgagctggt caccgccgac
     2101 gtggaggagc agcaccaggg cggcgtcatg caggcgctgg gcgagcgcaa gggcgagctg
     2161 acgaacatgg agcccgacgg ccgcggccgc gtgcgcctgg agtaccgcat ccccgcgcgc
     2221 gggctgatcg gattcaccaa cgagttcctg aacctcaccc gcggctcggg cctgatcagc
     2281 aacatcttcg acggctacga gccgcacaag ggcgagatcg gcggccgcaa gaacggcgtg
     2341 ctgatctcga tggacgacgg cgagatcttc acctacgcgc tgggcaagct cgacgaccgc
     2401 ggccgcatgt tcgtcaaggc cggcgacccg gtctacgagg gcatgatcgt cggcatccac
     2461 aaccgcgaca acgacctggt cgtcaacgcc acgcgcacca agcagctgac caacttccgc
     2521 gtctccggca aggaagacgc gatcaagatc acgccgccga tcgacctcaa cctcgagtac
     2581 ggcgtcgagt tcatcgacga cgacgagctg gtcgagatca cgcccaagag cgtgcgcctg
     2641 cgcaagcgct tcctgaagga gcatgaccgc aagcgtgcgt cgcgcgagaa cgcggcctga
     2701 gcgggcgcag gccgtcaccg aagagcgacc cgccgcgcgg cgaggtcgcc tttttcatgg
     2761 cgccggcgac gccagctgcg ccgcgatcgc cgcctggtcg atgaccgacc acacctggcg
     2821 gatgcggcct tgcgcgaact cgtagaagac gtgctcgtcg aagcgcacgc gtcgcccgtc
     2881 gacccgcagg ccgaagagct cgcccgcggg cgtgcagtcg aagcgcaggc ggcacgccac
     2941 gatcgggggc tggcaggcga gcagcgcgat ctcgaagcgc aggtcgggga tcgcccggac
     3001 gtcggcctcc agcatcgcgc ggtagcccgc caggccgagc gcgcggccat tgtggacggc
     3061 gtgctcgtcg acgaaatcgc ccaggcgtgc ccagtcgcgg gcgttgaggc agtcgacgta
     3121 ggcgcggtag gtgtcggcga gagcggtcgg ttccatggcg gcggcaagag aagggtcgag
     3181 cgctaccttc gcacggcgca caagaccgct gcggcgtgcg ggattcgggc gcggcagggc
     3241 ggcggcttcg cgcacgcgaa ctttgctcgc gcggcggcaa cgttttccgc gcgggatgcg
     3301 ccggcccggc gcttaagatg cccgcccggg acatcgaacg agacaaggag accgattgac
     3361 gaccgccatc ctccccgccg gcatgggcca cgcgcgcgcc gcgctgggca tggtcgtcgt
     3421 gacactgctc tggagcacgg cgggcgtcgt cagccgccac ctgcaggcgg ccgagagctt
     3481 cgaggtcacg ttcttccgca gtctgttcaa cctcgtgggg ctgggcgtcg cgctcacctg
     3541 gatgcgcggc gcgtcgctgt ggacggagct cgcgcgcgcg ccgcgggtcg tgtggatgtc
     3601 gggcgtgtgc tgggccgtga tgttcaccgc gttcatggtg gcgctgacga tgacgcgcgt
     3661 ggccaacgtg ctggtgacca tgtcgatcag cccgctgctg acggcgctgt tcgcgcgcgt
     3721 gttcctgcgc caccggctgc cgccgcggac ctgggtcgcg atcgcggtcg cgggcgcggg
     3781 catcgcctgg atgttcggcc gcaacatgca ggcggccgac gcgcgcagcc tggccggcat
     3841 ggcggtggcg atcctcgtgc cgttgtcgac ggcgctgaat tacacggtgc tccaccacgc
     3901 cggccgcgcg ccggcctcgg cggcgtcgtc cgcgcgcgag ctcgacgcgc ccggtgccga
     3961 cggcaccgcg acgccgggcg aggagcccga catgctcgcc gcggtgttga tcggcgcggc
     4021 gctgtcggcg ctcgtgacgc tgccgatcgc ctggccgttc cgggccacgc cgcacgacct
     4081 cgcgctgctg ttcggcctgg gcatcttcca gctcgccatc ccctgcctgc tgctggtgcg
     4141 gctcacgcgc gagctcgcct cgcccgaggt cgcgctgctg ggcctgctcg aggtgatctt
     4201 cggggtgctg tgggcgtgga tcggcgtcgg cgagtcgccc ggcagctccg cgctcgtcgg
     4261 cggcgcgctg gtcgtcggtg cactggcctt caacgagctg ctgagcctgc gcgcgcgcgg
     4321 cgccgcgcgc tccggggccc actgaaatcg cggcagcgac cgcggccgct tcccagaccg
     4381 attcctgtct cccatcccga agcccatgac cgatccgacc gccacgactt ccgccgcccc
     4441 gaccgtcctc gccgaacgcc gccggcatgt cggcgtgctc acgctcaacc ggccctcggc
     4501 gctcaacgcg ctgtcgctgg agatgatccg tgcgctcacg gcacagctgc gcgcctggcg
     4561 cgacgacccg gaggtcggcg ccgtcgtcct gcgcggcgcc aaccggcccg gcaagccgcc
     4621 cgcgttctgc gccggcggcg acatccgctt cttccaccag gcggcccgca ccgccgaccc
     4681 ggcgctgggc gacttcttca ccgaggagta cacgctcaac cacctgatcc acacctacgg
     4741 caagccgacc ttcgtcgtga tggacggcat cacgatgggc ggcggcatgg gcctcgccca
     4801 gggcgcgtcg ctgcgcatcg ccaccgagca tgcgaagctc gcgatgcccg agacgctgat
     4861 cggcctgttt cccgacgtcg gcggcggctg gttcctcgca cggctgccgg gccgactcgg
     4921 tgagttcctc ggtctcaccg ggctcacgct gggcgcgggc gatgcgctgg ccgtcggctg
     4981 ggccgacctc tgcgtgtcgg cggatcgcgt ggatgcgctg ctccaggcgc tggtcgatgc
     5041 gacgccggcc gacggcgtcg gcgcgctcac ggccgtccac gcgttcgcgg cggacgcggg
     5101 cgccggcacg ctcgccccgc accaggcgct gatcgagcgc gtcttctcgc agccgacgct
     5161 ggcggccatc gtcgccgcgc tgcaggccga ggagcagggc gacgacgcgg agttcgcgcg
     5221 cgcgaccgcg gccctgctcg ccaagcggtc gccgacgatg ctcgccgtca cgctggagca
     5281 ggtgcgtcgc gcgcgcacga tgagcctggc cgacgagctg cgcatggagc gcgacctcgt
     5341 ccaccactgc ttccacctcc ggccgccgga gcgcagcgag acgctcgagg gcatccgggc
     5401 cttggccgtc gacaaggacc acgcgccgcg ctggcagccc gcctcgatcg ccgacgtcga
     5461 cgcggccgag atcgcggcgt tcttcgagag cccctggacg ccgcaggcgc atccgctgca
     5521 cgcactggcg tgatcgccgg cgcagccgta tcgccgcggc tcggccgggc atcgcggttg
     5581 ctgcgatgca ggcgagctcc cggccccgcg cgtcgtttcg cggcgcccga cggtttgccg
     5641 gcgtgacggc cgcgcgccgg gagcttcccc ccgctgccga ggtgcgccat gacccagaca
     5701 ctgccgaccc tgttgctcgc cgcgctggcc gccgtctgct cggcggccgc gccgtgcgtg
     5761 gccagcgccg aggactccaa ggatgcgacc tacgagcccg acaagggccc gaatcccggc
     5821 ctgaccaccg acgcacgcta ccaccgggcg aagcgcaacg cggacaagcg cctgaaggac
     5881 agcctcgcgc agtgcgaccg catgggcgcc gccgaccgcg cgcagtgcat ccgcaacgcg
     5941 aaccagaccc acgacgcgga cgtcgcgcac gccgacgatt tcctggagcg cgcgccggag
     6001 acgcaggagc gctgacctgt cggcggggcg gcgccgcatc gcgggcgtcg agaagacggg
     6061 acgcccggcc gggcgctggg cgatgacgtg gacatggcgc cgccgacgtc gccggggact
     6121 cactggcccc ggtcatgcgc cgcggcgccg cgctggcaag ggcctgcgcg cgagcagcca
     6181 ggcccaggcg gccagcagca tcgtccaggc gggtgcttcg ggaatcgccg gcgggggcgg
     6241 accgccgacg ggctcctccc ctccgggcgg attgccgatg acgacgcagc agtcgtcgtc
     6301 gggcggcggg ccgacgaccg gctcgcccgg gccgccaccc cacagggatc cgccgtcgac
     6361 ggggccgccg ctgccgacgg gcggcggcag accgtcgatc ggcggccacg gcaggaaatc
     6421 ctgcgcgtcg gagggcgagg cagggggagt cggcgcgacc ttgggcggcc cggccgcggg
     6481 ctcgatgtcg atccggcctt ccttggcggg cttcccgcgg cgcgtcacga gcgcgacgtt
     6541 gtggcaggcc agcggcacca tcacgcacgt gtcgccgacg cagtagacca gcgcacgctc
     6601 cacgtggctc gccgaccagg tcgagcgctc gacccttccc cggcagacgc ggccgtggcc
     6661 gctgtgcatc gcgcgcatgt cctcgtaggc gccatcgtcg cccgagatgt cgtcgcgcgt
     6721 gatgcgcacg acgtcgctcg gacggtgcgc ctgcatcatc gcgcgcaagc gggcgcgggt
     6781 gtcttcgggg agggcgaaat cggcgagcac cgcgaccggg tcgtcgcgat acggatccgc
     6841 tcccggacgg gcccacgaac aatcgggcga gggcgcgacg ccagccgtcg cggcaagcgc
     6901 gttggccaag atcaggtgca atcccatcgg ttctcgttcc ggacgggccc accccgccat
     6961 ggcccctccg aggaggtgtc ccggcaggcg acgtaccagc gtcgggggcc cggccgcatc
     7021 ggtgcgattc gtcgcgggcg acgggcaccg cgcgcggccg ctcgacgcga gcgggcaacg
     7081 agcttgcccc gaccgccgcg acacgttcgc gcagtcgatc cggcggcaga acgacgagca
     7141 ggtgtgctgc gtctgctccg acgtcgcgat caagcgctgc ggccgacgac gacgcgccct
     7201 cgccgcgacg agcgggcgca cgcctgcgcc ccatcgcccg ccggcatcga ccgaggaaac
     7261 tgcatgaccg tgaagcatcc gcacccagat cacgatcacg aggccggcca ccaccccggc
     7321 cacagcggcc atgccggcca cgaccacgcc gccggcgcca acgagaggtc gctgaagatc
     7381 gccttcgtcc tgaccttcgc gttcctggtc gtcgaactgg tcggcggcat cgtcgcgcac
     7441 agcctggcgc tgctgtccga cgccgcgcac atgttcaccg acaccgcggt gctcgccatc
     7501 gcgttgctgg cgatccgcat ctcgaggcgc ccggctgaca agaagcgcac cttcggctac
     7561 caccgcttcg aaatcctggc cgcggccttc aacgcgatcc tgttgctgtt cgtggcggcc
     7621 tacatccttc gggaggccta tctgcgcatc gaggcgccgc ccgagatcca gtccaacctc
     7681 atgctggtca tcgccgtcgt ggggctcctc gtcaacctgg ggagcatgac ggtgctgcgc
     7741 agcgggcagc aggaaagcct gaacgtcaag ggcgcctatc tcgaggtctg ggccgacatg
     7801 atcggctccg tcggcgtgat catcggggcc ggcctgatca agctcaccgg ctgggcctgg
     7861 atcgactcgg cggtggccat cctgatcggc ctgtgggtgg tgccgcggac gatgacgctg
     7921 ttgaagacga gcgtcaacat cctgctggac ggcgtgcccg aggacatcga catcgagaag
     7981 gtggaggcga ccctgcgggc gctgccgggc gtgaggagct tccacgacct gcacgtgtgg
     8041 tcagtgagca gcggcaaggc gacgctgacc gtgcacctgg tcatcgaccc ctcgacggac
     8101 ggcgaggcgg aagtcctgcc actcgcgcgc gagcgtctgg atcggggatt cgggctgcgg
     8161 cacctcacca tccagtgcga gacccacccc tgcggcatgg ccgacccggc cggacactcc
     8221 tagcatggcc ggcgcttgcg ccggcggcgg cggtcgccaa gcgctatact tgacgccaaa
     8281 atgtctttcg aatcaacggc ttacagcccc tggcgcttgt ccgtggcgcc gatgatggac
     8341 tggacggacc gccactgccg cttcttccat cgcctgatca cccacggcac gctgctctat
     8401 accgagatgg tcaccacggg cgcgctgctg cacggcgatc ggccgcgcca tctcgacttc
     8461 agccacgagg aacacccggt tgcgctgcag ctgggcggca gcgagcccga ggcgctcgcc
     8521 gcctgcgcga agctggcgca ggactggggc tacgatgagg tcaacctcaa ttgcggctgc
     8581 cccagcgagc gcgtgcagcg cggcgcgttc ggcgcctgcc tgatgcgcga ggccgagctg
     8641 gtgcgcgaca gcgttgccgc gatgcgggac gcctgcgcgc tgccggtgac cgtcaagcac
     8701 cgcatcggcg tcgacaaggt cgagagctac gagttcgtgc gcgacttcgt cggcaccgtg
     8761 gcgcagggcg ggtgcatggt gttcatcgtg cacgcccgca acgcctggct gcaggggctg
     8821 agccccaagg acaaccgcac gatccccccg ctgcgctacg agttcgtcta tcgcctcaag
     8881 cgcgacttcc cggacttcac gatcgtgctg aacggcggcg tgaagggcga cgacgagatc
     8941 gcgcgccacc tggagcacgt cgacggcgtg atgatcggcc gggaggccta tcacgacccg
     9001 tggtgcatgg ccgactggga cgcgcgcttc ctcggccggc cggccccggg cctcgaccgg
     9061 gccgaggtcg aggcgcggat ggtggcctat ctctccggcc tgcaggcggc cggcgtgccg
     9121 tggtcgcagg cctcgcgcca catgctgggc ctgtggaacg gcgtcaaggg cgcgcgtcgc
     9181 tggcgccagg tctggtccga ccacaaggtc aagggcctcg ctccggcgga ggtgtcgcgc
     9241 ctcgcgcacg aaggcttcga gccggcggat ggcgcgccga cgcggctggc cgcggcgtga
     9301 acgatcgacg cgcgccgcgt ccttgg
//
LOCUS       DASVLN010000344         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_339860, whole genome shotgun sequence.
ACCESSION   DASVLN010000344 DASVLN010000000
VERSION     DASVLN010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339860"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..654)
                     /locus_tag="VF453_08520"
     CDS             complement(7..654)
                     /locus_tag="VF453_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7687736.1"
                     /translation="MSLENYWLFLPACFAINMAFGPNNVLSLSNGAREGARLSVAASI
                     GRLVAFAAMIAVAGLGLGALLLASQALFTVLKLGGAAYLVWIGIKLLRADPAALARAD
                     DAGPGERTAAAGKWRRLAKQEFLVAAGNPKAILVFTAFFPQFVDRAHYAASFALLGAT
                     FLVLELVAIAVYALLGARLGTLSGGARRLRWFNRASGSLMIGFGLMLALVRRPSH"
     gene            complement(690..1478)
                     /gene="map"
                     /locus_tag="VF453_08525"
     CDS             complement(690..1478)
                     /gene="map"
                     /locus_tag="VF453_08525"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7687737.1"
                     /translation="MRKIHIRTPDEIAKARAAGALAADVLRMIAPHVKPGVTTDQLDR
                     LCHDHIVGVQRAIPANVGYQGYPKTVCASVNHVVCHGIPSDKVLKKGDIVNIDVAVIK
                     DGWFGDTSRMYYVGEPSTLARRLVETTYAAMRAGIRQVRPGATLGDVGAAIQEVATAA
                     GFSIVRDYGGHGIGQVYHDEPHVHHYGHRGEGLRLEPGMIFTIEPMLNAGKAGTRELA
                     DGWTVVTRDRSLSAQWEHMVAVTPEGFEVLTAWPDGTGSYPPIA"
     gene            complement(1540..1818)
                     /locus_tag="VF453_08530"
     CDS             complement(1540..1818)
                     /locus_tag="VF453_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParD-like family protein"
                     /protein_id="HEX7687738.1"
                     /translation="MGIVKISDRMHDQLRGASEALSRSINAQAEHWMRVGMLAELHPG
                     FTYADICQLMLRAARTGEDPLALAPPEVEPASAKASATAAKAIRRAAR"
     gene            complement(1897..2913)
                     /locus_tag="VF453_08535"
     CDS             complement(1897..2913)
                     /locus_tag="VF453_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7687739.1"
                     /translation="MGADDAFLHDAYSQAVSGVVERVGPAVAAVHPMALPGAKGGGQD
                     AARPLGGGSGFLFTPDGFVLTNSHVVRAGAAADSARPAFRHRLSLADGREFDARWVGD
                     DPDTDLAVLSIDGLSRGALSHAPLGRSAGLKRGQVAIAIGNPLGFEHTVTAGIVSALG
                     RSMRASTGRLIPDVIQTDAALNPGNSGGPLLDSRGEVIGVNTAIIRGAQAICFAVAID
                     IAAWVIPQLLQHGRVRRGFLGIAGQTQAIDRRVVLAYELDQPSGVRVTAVEPGSPAAR
                     VGVREGDLVVGLDGVATPSVDALHQALDASRVNRDCVLKLIRGTRTPAPLYLTVRPLE
                     VLRG"
     gene            complement(2973..>3255)
                     /locus_tag="VF453_08540"
     CDS             complement(2973..>3255)
                     /locus_tag="VF453_08540"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7687740.1"
                     /translation="AELGYRVDQAESGRGIASRAVALALDEAFGPLALWRVEAVVRPE
                     NAGSVRVLERNGFVRFGHSRRAFRLGGTWYDRLLFERHRDGALDGDWIP"
BASE COUNT          452 a         1199 c         1175 g          429 t
ORIGIN      
        1 aaccgctcag tgcgacggcc ggcgcaccag cgcgagcatc agcccgaagc cgatcatgag
       61 cgagccgctc gcgcggttga accagcgcag ccgccgcgcg ccgcccgaca gcgtgcccag
      121 ccgcgcgccg agcagcgcgt acaccgcgat ggcgaccagc tccagcacga ggaaggtcgc
      181 gcccagcagc gcgaagctcg cggcgtagtg cgcgcggtcg acgaactgcg ggaagaacgc
      241 ggtgaacacg aggatcgcct tcgggttgcc ggcggcgacg aggaattcct gcttcgcgag
      301 gcggcgccac ttgcccgcgg ccgccgtgcg ctcgcccggg ccggcgtcgt ccgcgcgcgc
      361 cagcgccgcc ggatccgcgc gcagcagctt gatgccgatc cacactaggt aggccgcgcc
      421 gcccagcttc agcaccgtga agagcgcctg cgaggcgagc agcagcgcac ccagcccgag
      481 ccccgcgacc gcgatcatcg ccgcgaaggc gaccagccgc ccgatcgacg ccgccaccga
      541 cagccgtgcc ccctcgcgcg cgccgttgga cagcgacagg acgttgttgg gtccgaacgc
      601 catgttgatg gcgaagcagg cgggcaggaa gagccagtag ttctcgaggg acatgcgacg
      661 ctccggatgg tgcggagcat cgtagcggct cacgcgatcg gcggatagct cccggtgccg
      721 tccggccacg ccgtcagcac ctcgaagccc tcgggcgtga ccgcgaccat gtgctcccac
      781 tgcgcggaca gcgagcggtc gcgcgtgacg accgtccagc cgtcggcgag ctcgcgcgtg
      841 cccgccttgc cggcgttgag catgggctcg atcgtgaaga tcatgcccgg ctccagccgc
      901 aggccctcgc cgcggtggcc gtagtggtgc acgtgcggct cgtcgtggta gacctgtccg
      961 atgccgtggc cgccgtagtc gcgcacgatg ctgaagccgg cggccgtggc gacctcctgg
     1021 atcgcggcgc ccacgtcgcc cagcgtcgcg ccgggacgca cctggcggat gcctgcgcgc
     1081 atcgcggcgt aggtcgtctc gaccagccgg cgcgcgagcg tcgagggctc gccgacgtag
     1141 tacatccggc tcgtgtcgcc gaaccagccg tccttgatca cggcgacgtc gatgttgacg
     1201 atgtcaccct tcttcagcac cttgtccgac gggatgccgt ggcacacgac gtggttgacc
     1261 gaggcgcaga ccgtcttcgg gtatccctgg tagccgacgt tggccgggat cgcccgctgc
     1321 acgccgacga tatggtcgtg gcagaggcgg tcgagctggt cggtggtgac gccgggcttc
     1381 acgtgcggcg cgatcatgcg cagcacgtcg gccgcgagcg cgccggccgc gcgcgccttg
     1441 gcgatctcgt cgggcgtgcg gatgtggatc ttcctcatgc gcggacctcc gccctgcgca
     1501 cggcggcgcg agcccggccc gcggcggcct ggcgggcgat catcgcgccg ccctgcggat
     1561 cgccttggcc gccgtcgccg acgccttcgc gctcgccggc tcgacctcgg gcggcgccag
     1621 cgcgagcgga tcctcgccgg tgcgcgcggc gcgcagcatc agctggcaga tgtcggcgta
     1681 ggtgaagccg ggatgcagct cggccagcat gcccacgcgc atccagtgct cggcctgggc
     1741 gttgatggag cggctcagcg cctcgctggc gccgcgcagc tggtcgtgca tgcggtcgga
     1801 gatcttgacg atgcccatgg gtgttctcgc tatacgtttt gtatatgaaa cgtatattag
     1861 ccgatggggc atcgccgccg caagcggccg tgctcctcag ccgcgcagca cctcgagcgg
     1921 acgcaccgtg aggtacagcg gcgccggcgt gcgcgtgccg cggatgagct tgagcacgca
     1981 gtcgcggttc acgcggctcg cgtcgagcgc ctggtgcagc gcgtcgacgc tgggcgtggc
     2041 gacgccgtcc agcccgacca ccaggtcgcc ctcgcgcacg cccacccgcg cggccgggct
     2101 gcccggctcg accgccgtga cgcgcacgcc gctcggctgg tcgagctcgt aggcgagcac
     2161 cacgcggcgg tcgatcgcct gcgtctggcc ggcgatgccc aggaagccgc gccgcacgcg
     2221 tccgtgctgc agcagctgcg ggatcaccca ggccgcgatg tcgatcgcca ccgcgaagca
     2281 gatcgcctgc gcgccgcgga tgatcgccgt gttgacgccg atcacctcgc cgcgcgagtc
     2341 cagcagcggg ccgccggagt tgcccgggtt cagcgccgcg tcggtctgga tcacgtcggg
     2401 gatcagccgc ccggtcgacg cccgcatgct gcgcccgagc gcgctcacga tgcccgcggt
     2461 gacagtgtgc tcgaagccga gcgggttgcc gatcgcgatc gccacctgcc cgcgcttcag
     2521 gcccgccgag cggcccagcg gcgcgtgcga caacgcgccg cgcgacaggc cgtcgatgct
     2581 caggacggcc aggtcggtgt ccggatcgtc gccgacccag cgcgcgtcga actcgcggcc
     2641 gtcggcaagc gacaggcggt ggcggaacgc cggccgtgcc gagtccgccg ccgcgcccgc
     2701 gcgcacgacg tgcgagttgg tcagcacgaa gccgtccggc gtgaagagga agcccgagcc
     2761 gccgcccagc ggccgcgccg catcctgccc gccgcccttc gcgcccggca gcgccatcgg
     2821 gtgcaccgcc gcgaccgcgg gcccgacgcg ctccaccacg ccgctcaccg cttgcgagta
     2881 ggcatcgtgc aggaacgcgt cgtcggcacc catggcatct ctccttcgag ggtcgaagct
     2941 gaggctgggg tacgtggatt caagagcgaa ggctacggaa tccagtcgcc gtccagcgcg
     3001 ccgtcgcggt ggcgctcgaa cagcagccgg tcgtaccagg tgccgccgag ccggaaggcg
     3061 cggcgcgagt ggccgaagcg gacgaagccg ttgcgctcga ggacgcggac ggagccggcg
     3121 ttctcgggcc ggacgaccgc ctcgacgcgc cacagcgcga gcggcccgaa ggcttcgtcc
     3181 agggccagcg ccaccgcccg cgacgcgatg ccgcggccgc tctcggcctg gtcgacgcgg
     3241 tagcccagct ccgcg
//
LOCUS       DASVLN010000345         3672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_448514, whole genome shotgun sequence.
ACCESSION   DASVLN010000345 DASVLN010000000
VERSION     DASVLN010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448514"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..3023)
                     /gene="ppc"
                     /locus_tag="VF453_08545"
     CDS             complement(192..3023)
                     /gene="ppc"
                     /locus_tag="VF453_08545"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX7687741.1"
                     /translation="MPRTAATATPAKTPRNGANSKVNASASDKDRPLVEDIRLLGRLL
                     GEVIREQEGTAAFELIERVRQLSVAYRIHKDASAGRVLDRLLKNLSADQTVSVIRAFS
                     YFSHLANIAEDRQHVRRRAHHEREGNLQEGSLAMAFERLHDADHRAAEIAAALNRAYI
                     SPVLTAHPTEVQRKSILDAERAIAGLLAERDELLSERAREENTALMRARITQLWQTRM
                     LRYTKLTVADEVENALSYYHATFLRQIPRMYREIEQALPGHEVAPFFRMGNWIGGDRD
                     GNPNVTAGTLRHALSRQSETALRHYLYEVHALGAELSISSLLSPVTPEMQALAERSPD
                     HNAHREDEPYRRALVGIYARLAATLHALTGTEALRHAVAPQDPYASPAEFIAELEVIA
                     RSLRSHHAEALIAPRLAPLVRAAQVFGFHLATVDLRQSSDQHEAVIAELLRTARLEPD
                     YAALGEPERRTLLLALLDDARPLRVRGAEYSALAQGELEIFETALAMRASFGPQAIRH
                     YIISHTEDVSDLLEVLLLQKECGLMRGTLDDGAVADLIVSPLFETIADLRNGAHIMQQ
                     FYELPGVADLVKRSGAEQDIMLGYSDSNKDGGFFTSNWELYRTEVALVALFSQLEQTH
                     GIRLRLFHGRGGTVGRGGGPSYQAIVAQPPGTVNGQIRLTEQGEVIASKYANPDIGRR
                     NLETLVAATLEATLLHTTKTPPKAFLEAADALSQASFAAFRKLVYETPGFTDYFFSAT
                     PIREIAELNIGSRPASRKSTRRIEDLRAIPWGFSWGQCRVALPGWAGFGSGVEAFLGD
                     EPAEHARRLELLRRMYRQWPFFRTLLSNLDMVLAKSDLRIAARYVDLVDDRKLGKRIF
                     AAIKAEWERTQAMLGEITGEESRLQANPSLARSIEHRFPYLDPLNHLQVELMRRYRQQ
                     READATNDRLRRGIHLSINGIAAGLRNTG"
     gene            3142..>3672
                     /gene="hemC"
                     /locus_tag="VF453_08550"
     CDS             3142..>3672
                     /gene="hemC"
                     /locus_tag="VF453_08550"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX7687742.1"
                     /translation="MNNIVIATRESRLALWQARHVQALLGARLGIGASILGMTTRGDQ
                     ILDRTLAKVGGKGLFVKELELALEDGSAHLAVHSLKDVPMELPQGFVLAAVLEREDPR
                     DAFVSPRFASLDALPRGARVGTSSLRRVAQLLALRPDLRFAPLRGNLDTRLRKLDEGQ
                     YDAIVQAAAGLVRLGLE"
BASE COUNT          484 a         1229 c         1404 g          555 t
ORIGIN      
        1 cgccgcggcg agggccgcga ggcgggcgcc cgcgcggtgg cggattcggg caagcgcagg
       61 gacgaaggcg aggtcggacg gggttcggag cacatcgcct tatcggcccg gtcgccgggc
      121 gcttgagcgc gcacgcgccg acgagcgaat cccggagggc gcgcgtgcga aagtgcgcgc
      181 gccggcggat ttcagccggt gttgcgcagg cccgccgcga tgccgttgat cgacaggtgg
      241 atgccgcgcc gcagccggtc gttggtcgcg tcggcctcgc gctgctggcg gtagcggcgc
      301 atcagctcga cctgcaggtg gttcagcgga tcgaggtagg ggaagcggtg ctcgatcgag
      361 cgcgcgagcg acgggttggc ctgcagccgc gattcctcgc cggtgatctc gcccagcatc
      421 gcctgcgtgc gctcccactc ggccttgatc gccgcgaaga tgcgcttgcc gagcttgcgg
      481 tcgtccacga ggtcgacgta gcgcgcggcg atgcgcaggt cgctcttggc aagcaccatg
      541 tccaggttgg acagcagcgt gcggaagaac ggccactggc ggtacatgcg gcgcagcagc
      601 tcgaggcggc gggcgtgttc cgcgggctcg tcgccgagga aggcctcgac gccggagccg
      661 aagccggccc agcccggcag cgcgacgcgg cactggcccc acgagaagcc ccacgggatc
      721 gcccgcaggt cctcgatgcg gcgcgtcgac ttgcgcgagg ccggccgcga gccgatgttg
      781 agctcggcga tctcgcggat cggcgtggcc gagaagaagt agtcggtgaa gcccggggtc
      841 tcgtagacga gcttgcggaa agccgcgaag ctcgcctggc tcagcgcgtc ggcggcctcg
      901 aggaaggcct tgggcggcgt cttggtcgtg tgcagcagcg tcgcctcgag cgtcgcggcg
      961 accagcgtct cgaggttgcg gcggccgatg tccgggttgg cgtacttcga cgcgatcacc
     1021 tcgccctgtt cggtcaggcg gatctggccg ttgaccgtgc cgggcggctg cgcgacgatc
     1081 gcctggtagc tcgggccgcc gccgcggccc accgtcccgc cgcggccgtg gaacaggcgc
     1141 aggcggatgc cgtgcgtctg ctcgagctgc gagaagaggg cgacgagtgc gacctcggtg
     1201 cgatagagct cccagttgct cgtgaagaag ccgccgtcct tgttgctgtc gctgtagccg
     1261 agcatgatgt cctgctcggc gccgctgcgc ttgacgaggt cggcgactcc gggcagctcg
     1321 tagaactgct gcatgatgtg cgcgccgttg cgcaggtcgg cgatggtctc gaacagcggc
     1381 gagacgatca ggtcggccac ggcgccgtcg tccagggtgc cgcgcatcag gccgcactcc
     1441 ttctgcagca gcagcacctc cagcaggtcg ctcacgtcct cggtgtgcga gatgatgtag
     1501 tggcggatcg cctgcggccc gaagctcgcg cgcatcgcga gcgcggtctc gaagatctcg
     1561 agctcgccct gcgccagtgc cgagtactct gccccgcgca cccgcagcgg gcgggcgtcg
     1621 tccagcaggg ccagcagcag cgtgcggcgc tcgggctcgc cgagggcggc gtagtcgggc
     1681 tccaggcgcg cggtgcgcag cagctccgcg atcacggcct cgtgctggtc ggagctctgg
     1741 cgcaggtcga ccgtcgcgag gtggaagccg aagacctggg ccgctcgcac cagtggcgcg
     1801 aggcgcggcg cgatcagggc ctcggcgtgg tgcgaccgca gcgagcgagc gatgacctcg
     1861 agctccgcga tgaactcggc gggcgaggcg tacggatcct gcggcgccac cgcgtggcgc
     1921 agcgcctcgg tgccggtgag cgcgtgcagc gtcgcggcca ggcgcgcgta gatgccgacc
     1981 agcgcgcggc ggtagggctc gtcctcgcgg tgcgcgttgt ggtcgggcga gcgctcggcc
     2041 agcgcctgca tctcgggcgt cacgggcgac agcaggctcg agatcgacag ctcggcgccg
     2101 agcgcgtgca cctcgtacag gtagtggcgc agcgcggtct cggactggcg cgacaacgcg
     2161 tggcgcagcg tcccggcggt cacgttcggg ttgccgtcgc gatcgccgcc gatccagttg
     2221 cccatgcgga agaagggcgc gacctcgtgg ccgggcagcg cctgctcgat ctcgcggtac
     2281 atgcgcggaa tctggcgcag gaaggtcgcg tggtagtacg acagcgcgtt ctcgacctcg
     2341 tcggccacgg tcagtttggt gtagcgcagc atgcgcgtct gccagagctg cgtgatgcgg
     2401 gcgcgcatca gcgcggtgtt ctcctcgcgc gcgcgctccg acaggagctc gtcgcgctcg
     2461 gccagcaggc cggcgatcgc gcgctcggcg tcgaggatgc tcttgcgctg cacctcggtc
     2521 gggtgggccg tcagcaccgg ggagatgtag gcgcggttca gcgcggcggc gatctcggcg
     2581 gcgcggtggt cggcgtcgtg caggcgctcg aaggccatcg cgagcgagcc ttcctgcagg
     2641 ttgccctcgc gctcgtggtg ggcgcggcgg cgcacgtgct ggcgatcctc cgcgatgttg
     2701 gccaggtgcg agaagtagct gaacgcgcgg atcacgctga cggtctggtc ggccgacagg
     2761 ttcttcagca gccgatcgag cacgcggccg gcgctcgcgt ccttgtggat ccgataggcg
     2821 accgacagct ggcgcacgcg ctcgatcagc tcgaaggcgg ccgtgccttc ctgctcgcgg
     2881 atcacctcgc cgagcagccg gccgagtaac cggatgtcct cgaccagcgg gcgatccttg
     2941 tcgctcgccg aggcgttcac cttggaattt gccccattcc tgggcgtctt ggcgggcgtg
     3001 gcggtggcgg cggtgcgagg catcgatgtg ttccgattga tgtaaccgaa ttaccaatcg
     3061 ccaagtgtag cggggcgcgc ggcggccggg aagggcggat gcagcgcccc cgcgcgcgcc
     3121 cgcgtcgcct atgatccgcg catgaacaac atcgtcatcg cgacgcgcga aagccggctc
     3181 gcgctgtggc aggcgcggca cgtgcaggcg ctgctgggcg cgcgcctcgg catcggggcc
     3241 tcgatcctgg gcatgaccac gcgcggcgac cagatcctcg accgcacgct cgccaaggtc
     3301 ggcggcaagg gcctgttcgt caaggagctg gagcttgcgc tggaggacgg ctccgcacac
     3361 ctggccgtgc actcgctcaa ggacgtgccg atggagctgc cgcagggctt cgtgctcgcc
     3421 gcggtgctgg agcgcgagga tccgcgcgac gccttcgtct cgccgcgctt cgccagcctc
     3481 gacgcgctgc cgcgcggcgc gcgcgtgggc acgtccagcc tgcgccgcgt cgcgcagctg
     3541 ctcgcgttgc ggcccgacct gcgcttcgcg ccgctgcgcg gcaacctcga cacccgcctg
     3601 cgcaagctcg acgagggcca gtacgacgcg atcgtgcagg cggcggcggg gctggtgcgc
     3661 ctcggcctgg ag
//
LOCUS       DASVLN010000346         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_71217, whole genome shotgun sequence.
ACCESSION   DASVLN010000346 DASVLN010000000
VERSION     DASVLN010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71217"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1621
                     /gene="murC"
                     /locus_tag="VF453_08555"
     CDS             191..1621
                     /gene="murC"
                     /locus_tag="VF453_08555"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX7687743.1"
                     /translation="MKHAVKKIHFVGVGGAGMSGIAEILHNLGYEVSGSDQSDSATAR
                     RLADLGLQVFVGHDAAHIGDAQAVVVSTAVKGDNPEVIAARARRVPVVPRAVMLAELM
                     RLRKGIAIAGTHGKTTTTSLVTSVLAEAGVDPTFVIGGRLNSAGANSRLGAGEYIVVE
                     ADESDASFLNLMPILAVVTNIDADHMDTYGHDFARLKAAFVEFLHRMPFYGAAIVCGD
                     DPGVRSIVPMISRPVITYGTGEDVQVRAVDVRALPGGQMAFTCQRRNGVRMPDFDVVL
                     NLPGEHNVRNALAAIAVATELELPDAAIQRALAGFTGVGRRFQRYGELPVDADKGGGT
                     FTLIDDYGHHPVEMAAVIAAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVMGQADA
                     VLLTEVYAAGESPIVAADGRSLARALRVAGKVDPVFVDEVGELAAAIVDHARDGDVVI
                     SMGAGSIAAVPARVAEMKALRNGGAL"
     gene            1618..>2542
                     /locus_tag="VF453_08560"
     CDS             1618..>2542
                     /locus_tag="VF453_08560"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX7687744.1"
                     /translation="MNTTPLTQEQIEGLGKVAVLQGGTSAERLISQLSGQGVLAALRS
                     QGVDAHAFDPAERPLEDLKREGFDRVFIALHGRHGEDGTVQGALELLGIPYTGPGVMA
                     SSIAIDKVMTKRIWSAVGLPTPAWRVLRPSEQRHELVREVPDLLGLPLIVKPPREGSS
                     IGVTKVRGYSQMQDAVKLAAGYDADVLCEEFIDGEEYTCAVLGEGEAAFALPVVRIVA
                     PEGKYDLQNKYFTDEVKYLCPSGLPEAEEREIQRVTVEAYRVLGCRGWSRADVMVRAS
                     DRKPFLLEMNTSPGMTGHSLVPIAARGAGISY"
BASE COUNT          373 a          903 c          915 g          351 t
ORIGIN      
        1 gccgcgacgc gcgagcagct gctcgccacc gcgcaggccg cgcgcgcgct cgcccgcccg
       61 caggcggcgg cgcgcgtggc cgatgaactc gaaaggctcg tggcatgaat tccatcgctt
      121 caaaagttca acttttgaaa atcgggaagt gcgtcgcgaa gtgccgcgac tgcatgtcgg
      181 aggtgctcgc atgaagcacg ccgtcaagaa gatccacttc gtcggcgtcg gcggcgccgg
      241 catgtccggc atcgccgaga tcctgcacaa cctcggctac gaggtctcgg gctccgacca
      301 gtccgacagc gcgaccgcgc gccggctggc cgacctcggc ctgcaggtct tcgtcggcca
      361 cgacgccgcc cacatcggcg acgcgcaggc cgtcgtggtc tcgaccgccg tgaagggcga
      421 caaccccgag gtgatcgcgg cgcgcgcccg gcgcgtgccg gtggtgccgc gcgcggtgat
      481 gctggccgag ctgatgcggc tgcgcaaggg catcgcgatc gccggcaccc acggcaagac
      541 gacgacgacc tcgctcgtga ccagcgtgct cgccgaggcg ggcgtcgacc cgaccttcgt
      601 gatcggcggc aggctcaatt ccgcgggcgc gaactcgcgc ctgggcgcgg gcgagtacat
      661 cgtggtcgag gccgacgaat ccgacgcctc gttcctcaac ctgatgccga tcctggccgt
      721 cgtcaccaac atcgacgccg accacatgga cacctacggc cacgacttcg cgcgcctgaa
      781 ggccgcgttc gtggagttcc tgcaccgcat gccgttctac ggcgcggcca tcgtctgcgg
      841 cgacgacccc ggcgtgcgct cgatcgtgcc gatgatctcg cggcccgtga tcacctacgg
      901 caccggcgag gacgtgcagg tgcgcgcggt cgacgtgcgc gccctgcccg gcgggcagat
      961 ggccttcacc tgccagcggc gcaacggcgt gcgcatgccc gacttcgacg tcgtgctgaa
     1021 cctgcccggc gagcacaacg tgcgcaacgc gctggccgcg atcgcggtgg ccaccgagct
     1081 cgagctgccc gacgccgcga tccagcgcgc gctcgcgggc ttcaccggcg tgggccgtcg
     1141 attccagcgc tacggcgagc tgcccgtcga cgccgacaag ggcggcggca cgttcacgct
     1201 gatcgacgac tacggccacc acccggtcga gatggccgcg gtgatcgccg cggcgcgcgg
     1261 cgccttcccc gggcgccggc tcgtgctggc gttccagccg caccgctaca cccgcacgcg
     1321 cgactgcttc gaggatttcg tgaaggtgat gggccaggcc gacgcggtgc tgctgaccga
     1381 ggtgtacgcg gccggcgagt cgcccatcgt ggcggcggac ggccgctcgc tcgcgcgcgc
     1441 gctgcgcgtc gcgggcaagg tcgacccggt attcgtcgac gaggtgggcg agctcgccgc
     1501 cgcgatcgtg gaccacgcgc gcgacggcga cgtcgtcatc tcgatgggcg cgggctcgat
     1561 cgccgcggtg cccgcgcgcg tggcggaaat gaaggctttg cgaaacggag gcgcgctgtg
     1621 aacacgacgc cgctgaccca ggaacagatc gaaggactcg gcaaggtcgc cgtgctccag
     1681 ggcgggacgt ccgccgaacg gctgatctcg cagctgtcgg ggcagggcgt gctcgcggcg
     1741 ctgcggtcgc agggcgtgga tgcgcacgcc ttcgacccgg ccgagcgtcc gctcgaggac
     1801 ctcaagcgcg agggattcga ccgcgtcttc atcgcgctgc acggccgcca cggcgaggac
     1861 ggcaccgtgc agggcgcgct ggagcttctg ggcatcccct acaccggccc gggcgtgatg
     1921 gccagctcga tcgcgatcga caaggtgatg accaagcgca tctggagcgc ggtcggcctg
     1981 ccgacccccg cctggcgcgt gctgcggccg tcggagcagc gccacgagct cgtgcgcgag
     2041 gtgcccgacc tgctggggct gccgctgatc gtcaagccgc cgcgcgaggg ctcgtcgatc
     2101 ggcgtgacca aggtgcgcgg ctactcgcag atgcaggacg cggtgaagct cgccgccggc
     2161 tacgacgccg acgtgctgtg cgaggagttc atcgacgggg aggaatacac ctgcgccgtc
     2221 ctgggcgagg gcgaggccgc gttcgcgctg cccgtcgtgc gcatcgtcgc gccggaaggc
     2281 aagtacgacc tgcagaacaa gtacttcacc gacgaggtca agtacctgtg cccgagcggc
     2341 ctgccggagg ccgaggagcg cgagatccag cgcgtgaccg tcgaggccta ccgcgtcctc
     2401 ggctgccgcg gctggagccg cgccgacgtg atggtgcgcg cgagcgaccg caagcccttc
     2461 ctgctcgaga tgaacacgtc cccgggcatg accgggcact cgctggtgcc gatcgccgcg
     2521 cgcggcgcgg gcatcagcta cg
//
LOCUS       DASVLN010000347         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_126059, whole genome shotgun sequence.
ACCESSION   DASVLN010000347 DASVLN010000000
VERSION     DASVLN010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126059"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VF453_08565"
     CDS             complement(<1..1391)
                     /locus_tag="VF453_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7687745.1"
                     /translation="MRSLYLRIWLTVIVTLALFASVSGWLVQRHLDQERARNQAVLTE
                     RIAAWGDLIQRSLPAASAPVDVQAQALRDWSQRLRVPMALEDAQGHRVAASDSFIRRE
                     NDKASSRVVTYQIRLDDGRSLFVMRPAMLKPPVVDSEPGPEGGPGGPGGPGGLGERHV
                     QSFEGHGPPPLLLPGLPRGVQPGLSLLALVVVLFIAIAMGAYPFVRRLTRRLEALKRG
                     VEDFGEGALDRRVPVEGRDEVASLATSFNLAAERIEMLVRSHKSLLANASHELRSPLA
                     RLKMATSLLSDAAPGEAEREHLRAEINTDIAELDGLVDEVLLASRLEAAPQPEHLEAV
                     DLLALAVEEGARVDAGVEGSAVSVRGEERLLRRALRNLLENARRYGGGEIRVELVDAG
                     VSGVEVRVADNGPGVPEAYRERIFEPFFRLPGHAERAGGVGLGLALVRQIVERHHGRI
                     RCVAPPGGGGCFV"
     gene            complement(1564..2268)
                     /locus_tag="VF453_08570"
     CDS             complement(1564..2268)
                     /locus_tag="VF453_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7687746.1"
                     /translation="MNNPHLLLIDDDARLTRMVGDYLRTQGFDVDTAGTLAEGRMRLT
                     ASAYDALVLDLMLPDGDGLDFTRELRGQPATRRLPLMMLTARGEPTDRIVGLELGADD
                     YLPKPFEPRELLARVKALLRRASPEPVNEEVLRFGRLEIDLGAHEARLDGTRCDLTSH
                     QFDLLVVLARSPGRVLSRDQIMDALKGHPLEAFDRSIDVHISRIRSVIEDDPKNPKRV
                     LTVRGSGYLFARKQDA"
     gene            complement(2363..2878)
                     /locus_tag="VF453_08575"
     CDS             complement(2363..2878)
                     /locus_tag="VF453_08575"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX7687747.1"
                     /translation="MKPAKSSSRPVRLFGLALGCVLAGGVAFAAWAASDEAAGQRPPP
                     HRMGAMLPHGPMLDHLLDEAQASPTQRAQVHQILEAAEAPLKAERPAERADHEQIVQL
                     FSQPVVDPSQVEAVRQRMAVRRDADSRRMLQAMVDVSRVLSVDQRKVVLRAMAGGPRP
                     WHRGASAPAGE"
     gene            3114..>3485
                     /locus_tag="VF453_08580"
     CDS             3114..>3485
                     /locus_tag="VF453_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687748.1"
                     /translation="MTRSVDDLVAALRSLEPTDDESDNVHRLNEVFADLHVLADREEA
                     LPEFFFLLERHPQAAFGAPIPLVCELEKLSGYERHLAASLERQPTELAAMLANRLLNT
                     RQSPAARGEWLRRLGAVASDPR"
BASE COUNT          466 a         1275 c         1291 g          453 t
ORIGIN      
        1 atcacgaagc agccgccgcc gccgggaggc gcgacgcagc ggatgcggcc gtggtggcgc
       61 tcgacgatct ggcgcacgag cgcgagcccg agcccgacgc cgcccgcgcg ttcggcatgg
      121 ccgggcaggc ggaagaacgg ctcgaagatg cgctcgcggt acgcctcggg cacgccgggg
      181 ccgttgtcgg cgacgcggac ctccaccccg gagacgcccg cgtcgaccag ctcgacgcgg
      241 atctcgccgc cgccgtagcg gcgcgcgttc tccagcaggt tgcgcagcgc gcggcgcagc
      301 agccgctcct cgccgcgcac cgagacggcc gagccttcga cgcccgcgtc gacgcgcgca
      361 ccctcctcca ccgcgagcgc gagcaggtcg accgcctcca ggtgctcggg ctgcggcgcg
      421 gcctcgagac ggctggccag cagcacctcg tcgaccaggc cgtcgagctc ggcgatgtcg
      481 gtattgatct cggcgcgcag gtgctcgcgt tccgcctcgc cgggcgccgc gtcggacagc
      541 agcgacgtcg ccatcttcag ccgcgcgagc ggcgagcgca gctcgtggct ggcgttggcg
      601 agcaggctct tgtgcgagcg caccagcatc tcgatgcgct cggcggcgag gttgaagctg
      661 gtggcgagcg aggcgacctc gtcgcgaccc tcgaccggca ctcggcgatc gagcgcaccc
      721 tcgccgaagt cctcgacgcc gcgcttgagc gcctcgaggc gacgcgtgag ccggcgcacg
      781 aacgggtacg cgcccatcgc gatcgcgatg aacagcacga cgaccagcgc cagcaggctc
      841 agccccggtt gaacgccgcg cggcaggccc ggcagcagca gcggcggcgg gccgtggcct
      901 tcgaaggact gcacgtggcg ctcgcccagg ccgccggggc cgcccgggcc gcccgggccc
      961 ccctcggggc cgggctcgct gtccacgacg ggtggcttga gcatcgccgg ccgcatgacg
     1021 aacagcgagc ggccatcgtc cagccggatc tggtaggtca ccacgcgcga gctcgccttg
     1081 tcgttctcgc gccggatgaa ggaatcggac gccgcgacgc gatggccctg ggcgtcctcc
     1141 agcgccatcg gcacgcgcag gcgttgcgac cagtcgcgca gcgcctgcgc ctgcacgtcc
     1201 accggcgcgc tggccgccgg cagcgagcgc tggatcaggt cgccccaggc ggcgatgcgc
     1261 tcggtgagca cggcctggtt gcgcgcgcgc tcctggtcga ggtggcgctg cacgagccag
     1321 cccgagacgc tggcgaacag cgccagcgtg acgatcaccg tgagccagat ccgcaggtag
     1381 agcgaacgca cgcgagcagc cttgtcacca ccggaatggc gttggaacga aaacagcgcc
     1441 ggctggcggg ccgacgctgc gggggatggg caatgatggt acgtcggcgc cgcgcgcggc
     1501 ccgcgcgtcc gacggcgggg ttttcgaggg ttcgcgccgc gggcgcggcc ggcgcgacgg
     1561 cgatcaggcg tcctgcttgc gcgcgaagag gtagcccgag ccgcgcacgg tcagtacgcg
     1621 cttcgggttc ttcgggtcgt cctcgatcac gctgcggatg cgcgagatgt ggacgtcgat
     1681 cgagcggtcg aacgcctcca gcggatggcc cttgagcgcg tccatgatct ggtcgcggct
     1741 cagcacgcgg cccgggctgc gcgcgagcac gaccagcagg tcgaattggt ggctggtcag
     1801 gtcgcagcgc gtgccgtcca ggcgcgcctc gtgggcgccg aggtcgatct cgaggcggcc
     1861 gaagcgcagc acctcctcgt tgaccggctc cgggctcgcg cgccgcagca gcgccttgac
     1921 gcgggcgagc agctcgcgcg gctcgaaggg cttgggcagg tagtcgtcgg cgccgagctc
     1981 gaggccgacg atgcggtcgg tcggctcgcc gcgcgccgtc agcatcatca gcggcaggcg
     2041 gcgcgtggcg ggttggccgc gcagctcgcg ggtgaagtcg aggccgtcgc cgtcgggcag
     2101 catcaggtcg agcaccagcg cgtcgtaggc cgacgcggtc aggcgcatgc gtccttcggc
     2161 cagcgtgcct gcggtgtcga cgtcgaagcc ctgcgtgcgc aggtagtccc ccaccatgcg
     2221 cgtgaggcgc gcgtcgtcgt cgatcaggag caggtgcgga ttgttcatgg aagtggcggt
     2281 gccgcgcgcg ctcgcggcgg cggcccgggc gcccggggat gcgggagcgt aaccgatcgg
     2341 gtcgaggact gtcatccggc cgtcactccc ccgccggcgc cgacgcgccg cgatgccagg
     2401 ggcgcggccc accggccatg gcgcgaagca ccaccttgcg ctggtccacc gacagcaccc
     2461 ggctcacgtc gaccatcgcc tgcagcatgc ggcgcgagtc ggcgtcgcgg cgcacggcca
     2521 tgcgctggcg caccgcctcg acctgcgacg ggtcgacgac cggctgcgag aacagctgca
     2581 ctatctgttc gtggtcggcg cgctcggcgg ggcgctcggc cttcaacggc gcctcggccg
     2641 cctcgaggat ctggtgcacc tgcgcgcgct gcgtcggcga cgcctgcgcc tcgtcgagca
     2701 ggtggtccag catcgggccg tgcggcagca tcgcgcccat cctgtgcggc ggcggccgct
     2761 ggcctgccgc ctcgtcggaa gccgcccagg cggcgaaggc gacgccgccg gccagcacgc
     2821 atccgagggc gaggccgaac aggcgcacgg ggcgcgacga tgacttggca ggtttcatgg
     2881 agatctccct cgggcacggc gaggcccgcg aggcgcgggc cgagtgccgg ccatcgtgcc
     2941 gatgagcggt caaccgtcct tgcgcgcggc gtaaagatct gtgaatcggg gaacgccggc
     3001 gcgcgaccgg cgcaagccct gccccttcgc ccgccagtgc cttgccctgg cgcagtgcct
     3061 cggacgcctc gattgactac ccttgcgcgc atgccctctg ccgcgaccgc gccatgaccc
     3121 gctccgtcga cgacctcgtc gccgccctgc gctcgctgga gcccacggac gacgaatccg
     3181 acaacgtcca ccgcctcaac gaagtcttcg ccgacctgca cgtgctcgcc gaccgcgagg
     3241 aggccctgcc cgagttcttc ttcctgctcg aacgccatcc gcaggccgcg ttcggcgcac
     3301 cgatcccgct ggtctgcgaa ctcgagaagc tgtcgggata cgagcgccac ctggccgcgt
     3361 cgctggagcg ccagcccacg gagctcgcgg cgatgctcgc caaccggctg ctgaacacgc
     3421 gccagtcgcc ggcggcgcgc ggcgagtggc tgcggcggct cggcgcggtg gcgtccgatc
     3481 cgcgg
//
LOCUS       DASVLN010000348         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_287186, whole genome shotgun sequence.
ACCESSION   DASVLN010000348 DASVLN010000000
VERSION     DASVLN010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287186"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VF453_08585"
     CDS             <1..186
                     /locus_tag="VF453_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687749.1"
                     /translation="LAGQPVARASVDGPALALEMARRPARSPDWDRSRATDHAMVRKF
                     VDELRRRVARWNEGRDD"
     gene            206..454
                     /locus_tag="VF453_08590"
     CDS             206..454
                     /locus_tag="VF453_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687750.1"
                     /translation="MGYDAFERETNHGRPQATPRAIVAMRIHLSDEGRRIAEVLRSNT
                     LEPALTRKALASVEALPMPADVPDWPGEKSRRVGFLEA"
     gene            complement(590..979)
                     /locus_tag="VF453_08595"
     CDS             complement(590..979)
                     /locus_tag="VF453_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7687751.1"
                     /translation="MTTLPPAITRFDLPTTPPEIDHPREERREVGAPERRTWVLYESA
                     PAGLVAGIWECEVGRWRIQMDPTEHEYMVVLAGRARLHATDGTFTEVGPGEALVIPAG
                     FAGSFEVLERVRKHFVIVVGPDEARAA"
     gene            complement(1140..1583)
                     /gene="dtd"
                     /locus_tag="VF453_08600"
     CDS             complement(1140..1583)
                     /gene="dtd"
                     /locus_tag="VF453_08600"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX7687752.1"
                     /translation="MLSLIQRVREARVEIAGETVGAIGPGLLVLVCAEPEDTEGHADR
                     LVAKLLKLRVFGDESGKMNLSVQDLPDAGLLIVSQFTLAADTRGGNRPSFTGAAPPEL
                     GRRLYDLVLRLAAERHPRVASGRFGAEMQVHLVNDGPVTIPMRIQ"
     gene            complement(1651..2613)
                     /locus_tag="VF453_08605"
     CDS             complement(1651..2613)
                     /locus_tag="VF453_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7687753.1"
                     /translation="MQVGSYLKVSAGVAVATIALKAVAWWLTGSVSLLSDAIEGLVNL
                     AGAGFALAMVSWASQPPDEGHPYGHHKAEYFSSGFEGVLIVAAAIAIVGAAGERLAHP
                     AAVEALGPGLALSLGATAMNGMLAWAMLRKAAAARSIALEADARHLITDVWTSVGVVA
                     GLIGVRLTGWLWLDPVVALCVAGNIVREGVSLVWRSADGLMDMAVEPEVRAQIEKTLA
                     AFHGPAVRFDHVVTRRAGQRRFVDMHMHLPAAWTLGRAARLRAEVERALMRDVAGLRT
                     TIQLLPTDVEAYVEEFTDSPPGHDAGVPSTQIAPASPPASPMGH"
BASE COUNT          410 a         1017 c         1062 g          359 t
ORIGIN      
        1 ctcgccggcc agccggtcgc gcgcgcgagc gtcgacgggc cggcgctggc gctggagatg
       61 gcgcgtcgcc ccgcgcgcag tccggactgg gatcgctcca gggcgaccga ccacgcgatg
      121 gtgcgcaagt tcgtcgacga gctgcgccgc cgcgtcgcgc gttggaacga gggccgcgac
      181 gactgagccg cgtgcccgcc gccgcgtggg ctacgacgcc ttcgagcgcg agaccaacca
      241 cggccgcccg caggccacgc cgcgcgcgat cgtcgcgatg aggatccacc tgtccgacga
      301 ggggcgccgg atcgccgagg tcctgcgctc gaataccctc gagcccgcgc tcacgaggaa
      361 ggcgctcgcg agcgtcgagg cgctgcccat gcccgccgac gtccccgatt ggccgggcga
      421 gaaatcgcgc cgcgtgggct tcctggaagc ctgactgttc gagacgacgg gcatttcgcc
      481 gaagacgctg gcgctggccc cggaaggcgc ttcgagcctg cccatgggcc gcgtgtcggc
      541 aagcgcccgt cgacgggacg gcgtcccgcc gcgcgcgccc ggtgcgcgcc tatgccgccc
      601 gggcctcgtc gggcccgacg acgatcacga aatgcttgcg cacccgttcg agcacctcga
      661 acgagcccgc gaagccggcc ggaatcacca gcgcctcgcc ggggccgacc tcggtgaacg
      721 tgccgtcggt cgcatgcagg cgcgcgcggc ccgcgaggac gaccatgtac tcgtgctccg
      781 tcggatccat ctggatgcgc cagcggccga cctcgcattc ccagatgccg gcgacgagtc
      841 cggcgggcgc gctctcgtac agcacccagg tccgacgctc gggcgcgccg acctcgcggc
      901 gctcctcgcg cggatggtcg atctcgggcg gcgtcgtcgg caggtcgaag cgggtgatgg
      961 cgggcgggag cgtggtcatg gcgatgggct tgcggggagt cgtgaagtcg gaagaggcgg
     1021 tcgtgctggg atcgtacgtg gacgcggcgg gaatcagtgc gcgatgcggc acggtgtggg
     1081 cgcggaaccg ctcgccgacc cggggccgga tgccggtgcc cggtcagcga gccggaacgt
     1141 cactggatgc gcatcgggat cgtcaccggg ccgtcgttga ccaggtgcac ctgcatctcg
     1201 gcgccgaagc ggccgctcgc cacgcgggga tggcgctcgg cggccaggcg cagcaccagg
     1261 tcgtagaggc ggcgtccgag ctcgggcggc gccgcgccgg tgaagctggg ccggttgcca
     1321 ccgcgcgtgt cggccgcgag cgtgaactgg ctgacgatca gcagcccggc atcgggcagg
     1381 tcctggacgc tcaggttcat cttgcccgac tcgtcgccga agacgcgcag cttcagcagc
     1441 ttggcgacca ggcggtcggc atggccctcg gtgtcctcgg gctcggcgca gaccagcacg
     1501 agcaggccgg gcccgatggc accgacggtc tcgccggcga tctcgacgcg cgcctcgcgc
     1561 acgcgctgga tgagggacag catggggggc tccgtgggcg atgcggcgca ggttcggcgg
     1621 gggattggac gggacgggac gcggcggcgc tcagtgcccc atgggtgacg ccgggggcga
     1681 cgccggggcg atctgcgtgg aggggacgcc ggcgtcgtgg ccgggcgggg agtcggtgaa
     1741 ctcctcgaca taggcctcga cgtcggtagg cagcagctgg atcgtggtgc gcaggccggc
     1801 gacgtcgcgc atcagcgccc gctcgacctc cgcccgcagc cgcgcggcgc ggcccagcgt
     1861 ccaggcggcg ggcaggtgca tgtgcatgtc cacgaagcgg cgctggcccg cgcggcgggt
     1921 gacgacgtgg tcgaagcgca ccgcgggccc gtgaaacgca gcgagcgtct tctcgatctg
     1981 ggcccgcacc tcgggctcga cggccatgtc catcagcccg tcggccgaac gccagaccag
     2041 cgagacgccc tcgcgcacga tgttgccggc gacgcacagc gccaccaccg gatcgagcca
     2101 cagccagccc gtcaggcgca cgccgatcag gccggccacc acgcctacgg acgtccagac
     2161 gtcggtgatc aggtggcgcg cgtcggcctc cagcgcgatc gagcgcgcgg ccgcggcctt
     2221 gcgcagcatc gcccacgcca gcatgccgtt catggcggtg gcccccagcg acagcgcgag
     2281 gcccggcccc agcgcctcga ccgccgccgg gtgcgcgagc cgctcgcccg cggcgccgac
     2341 gatggcgatc gccgcggcca cgatcagcac gccctcgaag ccgctggaga agtactcggc
     2401 cttgtggtgc ccgtagggat ggccctcgtc gggcggctgc gaggcccagg agaccatcgc
     2461 cagcgcgaat cccgccccgg cgaggttcac gaggccctcg atcgcgtccg atagcaggct
     2521 caccgagccc gtcagccacc acgcgacggc cttgagcgcg atcgtcgcca ccgcgacgcc
     2581 cgccgacacc ttgagatagg aaccgacctg catgcgccca ttgtcgccgc tcccgcgcga
     2641 cgtccccggt ggcggaaggc ggggtttccg ggcgtttcga cgcgcgcggc ggcgtgtcgg
     2701 cctccggctc agagggtgta gctgaaatca acacttcaca cgctacagct tgcacaatag
     2761 ctgaagttgc gaggtgactg atgaacgaca agccgagaca gggcggacga ccgccgacgc
     2821 tcacgccaga gcatcgagcc gtactggt
//
LOCUS       DASVLN010000349         2929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_393445, whole genome shotgun sequence.
ACCESSION   DASVLN010000349 DASVLN010000000
VERSION     DASVLN010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393445"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VF453_08610"
     CDS             complement(<1..708)
                     /locus_tag="VF453_08610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7687754.1"
                     /translation="MSEASVPVPAGESSGTQQGWGAVAAQPDPRRWLILVVIGIAQLM
                     VVLDVTVMNLALPSAQKALGFSNADRQWIVTAYALSFGSLLLFCGRLADLIGRKVVFI
                     TGLIGFAVASAIGGASVNFAMLVTARACQGVFGAMLAPAALSLLTTTFSDPKERGKAF
                     GVYGAIAGAGSAVGLLLGGALTEYLSWRWCLYINLFFAIVAAAGGSVLLKRQPRTPGA
                     KLDVPGVVLVSGGMFCVV"
     gene            803..1513
                     /locus_tag="VF453_08615"
     CDS             803..1513
                     /locus_tag="VF453_08615"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7687755.1"
                     /translation="MTAQAEETSRDGAGERPLRRDAERNRQRILAAAAEVFTERGLDA
                     TLDEVARAAGVGVGTVYRRFPDKESLIAELFRDRIDALVTVAEEACAAQDPWQGLVGY
                     LEFAAATLAGDLGLRQLMMFGTYERDQVCYARDRMQPVITKLVERAQAAGELRADFRA
                     TDVKMIGFMLSSVAEYAASAAPEVWRRYLGMLVDGLRPSRPRSSALPVPAPTAEELAA
                     MMSARAQRSAPRRERYRG"
     gene            complement(1532..2155)
                     /locus_tag="VF453_08620"
     CDS             complement(1532..2155)
                     /locus_tag="VF453_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7687756.1"
                     /translation="MAGADLAEGTRLAAATSAWVCFEDETGQALRPPRARTWARRGRT
                     PVALVHAMGSSRVSAAGLACLKPGQPGRFFFRVRVHGRSKEQRRSLAEADYAELVTAA
                     HRTLGAPVILVWDNLNTHRSKVMREFTSAHPDWLTVFYLPAYAPELNPVEGAWSSMKS
                     GLGNLAPCTLAQLAALVRTRLRRIQHQPDLIAGFMKHTGLTLKPKPP"
     gene            complement(2119..2610)
                     /locus_tag="VF453_08625"
     CDS             complement(2119..2610)
                     /locus_tag="VF453_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7687757.1"
                     /translation="MRYPDGGGLTAEGRSRREKVRLQAAQMFQQGTDAVTAARVLRVS
                     TKSAYQWRRAWRAGGERALRSKGPGGSPCKLDDSQLARLRAALDAGPAAYGWQQDQRW
                     TLARAAQLAGRLFGVSYTLRGMSFLLHRLGFTPQVAAHRAAERDEAAVAQWREQTWPK
                     VRG"
BASE COUNT          447 a         1038 c         1033 g          411 t
ORIGIN      
        1 gaccacgcag aacatgccgc cggacacgag caccacacct ggcacgtcaa gcttggcgcc
       61 cggggtgcgc ggctggcgct tgagcagcac cgagccgccg gccgcggcga cgatcgcgaa
      121 gaacaggttg atgtagaggc accagcgcca cgacaggtac tcggtcaggg caccgccgag
      181 gagcaggccg accgcgctgc ccgcgccggc gatcgcgccg tacacgccga acgccttgcc
      241 tcgctccttc ggatcgctga acgtggtcgt gagcagggac agggcggcgg gcgccagcat
      301 cgcgccgaac accccctggc aggcacgggc ggtgaccagc attgcgaagt tcaccgacgc
      361 gccgccgatg gcggacgcga cggcgaaccc gatcaggccg gtgatgaaga cgaccttgcg
      421 gccgatcagg tcggcgagcc ggccgcagaa gagcagcagg ctgccgaacg acagcgcgta
      481 cgccgtcacg atccactgcc ggtcggcgtt gctgaagccg agcgccttct gcgccgacgg
      541 cagcgccagg ttcatcacgg tcacgtcgag cacgaccatg agctgcgcga tcccgatgac
      601 caccaggatc agccagcgcc gcgggtcagg ctgggcggcc accgcgcccc agccctgctg
      661 cgtcccgctg ctctcccccg ctggcacggg gacgcttgcc tcggacattc caaattccaa
      721 tctggaggat ctggttccgc ttatttgggc gagcatagca cgaagcggag gaacttcctc
      781 cgttcggggt acgctataag acatgacggc acaggcggag gaaacgtcac gcgacggcgc
      841 gggcgagcgt ccgctgcgcc gcgacgcgga gcgcaaccgg cagcgcatcc tcgccgccgc
      901 cgccgaggtg ttcaccgaac gcgggctcga cgcgaccctc gacgaggtcg cccgggccgc
      961 cggggtcggg gtcggcaccg tctaccggcg cttccccgac aaggagtccc tgatcgccga
     1021 gctgttccgg gaccggatcg acgccctcgt caccgtcgcg gaggaggcct gcgcggccca
     1081 ggacccgtgg cagggcctcg ttggctacct ggagttcgcc gccgccacct tggcaggcga
     1141 cctcggcctg cgccagctga tgatgttcgg cacctacgag cgggaccagg tctgctacgc
     1201 ccgcgaccgc atgcagcccg tgatcaccaa gctggtggag cgggcgcagg ccgccggcga
     1261 gctgcgcgct gacttccggg cgaccgacgt gaagatgatc gggttcatgc tctcctcggt
     1321 cgccgagtac gcggcctccg ccgcacccga agtctggcgg cgctacctgg gcatgctcgt
     1381 cgacggcctc cgcccgtccc ggccgcgcag ttccgcgctc cccgtgcctg cgccgaccgc
     1441 tgaggagctc gcggccatga tgagcgcgcg cgcccagcgc tcagctcccc gcagggagcg
     1501 atacagaggt tgaaagggca gggtctgggg ttcacggcgg tttgggtttg agggtgaggc
     1561 cggtgtgttt catgaagccg gcgatgaggt cgggctggtg ctggatgcgg cggagccggg
     1621 tgcggacgag ggcggcgagc tgggccaggg tgcagggggc gaggttgccg aggccgcttt
     1681 tcatgctgga ccaggcgcct tcgaccgggt tgagctcagg ggcgtaggcg ggcaggtaga
     1741 agaccgtgag ccagtccggg tgcgcgctgg tgaactcgcg catgaccttg ctgcggtggg
     1801 tgttgaggtt gtcccagacc aggatgaccg gggcgcccag ggtgcggtgc gcggcggtga
     1861 ccagctcggc gtagtcggct tcggccaggc tgcggcgctg ttccttgctg cggccgtgga
     1921 cgcggacgcg gaagaagaac cgccctggct gcccgggctt gaggcaggcc agcccggctg
     1981 cggagacgcg agacgagccc atggcgtgca ccagggccac cggggtgcgc ccgcggcggg
     2041 cccaggtgcg ggccctcggc gggcgcagcg cctggccggt ctcgtcttcg aagcagaccc
     2101 acgccgaggt cgccgccgct agcctcgtac cttcggccag gtctgctccc gccactgcgc
     2161 gaccgcggcc tcgtcccgct cggcggcccg gtgcgccgcg acctgcgggg tgaaccccag
     2221 ccggtgcagg aggaacgaca tcccgcgcag cgtgtaggac acgccgaaca gccggccggc
     2281 cagctgcgcg gcccgggcca gcgtccaccg ctggtcctgt tgccacccgt aagcggccgg
     2341 ccccgcgtcc agtgccgcgc gcagccgggc cagctggctg tcgtccagct tgcacgggct
     2401 cccgcccggc cccttcgacc gcagcgcccg ctctccgccg gcccgccagg cacgccgcca
     2461 ctggtacgcc gacttcgtgc tgacccgcag tacccgggcc gctgtcaccg cgtctgtgcc
     2521 ttgctggaac atctgcgcgg cctgcagccg caccttctcc cggcgggacc ggccctcagc
     2581 ggtcagcccg ccaccatccg gatacctcat gacaccggca taaacccggc acaaaccgca
     2641 atcacgcagg cgcgccgagt gtgctcaacc agacaaagac cccgaccttt caaggtctgt
     2701 agcacagggt tgcgcgcccg atatcatggc gggttttttg ccatcgggcg cgcaacccgg
     2761 tgacgagact cccgtcacat cgttccgtcc ttcggcggcc tgagcggcgt acggcgcgtg
     2821 cttcggacta cgctgcatgt aaacgccgct tgtcgtggcg tcaaccagac gcaagccaac
     2881 ggaccgagat cggaccccca gtggacctgt tcgagtatca ggctaagga
//
LOCUS       DASVLN010000350         4673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_448822, whole genome shotgun sequence.
ACCESSION   DASVLN010000350 DASVLN010000000
VERSION     DASVLN010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448822"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1363
                     /locus_tag="VF453_08630"
     CDS             116..1363
                     /locus_tag="VF453_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7687758.1"
                     /translation="MTDGSSLRAYPSFLRFWAGRLAGTMANQMLLVAVAWQMYDVTGS
                     ALSLGLVGLVQFVPALALVLVSGHVADRHDRRHIVAAAFLLQMAAALLLLVATREGRV
                     GRELILAVSLLLGVARSFQMTAQQALAPLLVPAGMLARALAFSSSGMQAAIIGGPALA
                     GVLYGGVLLHGFALTGGADVVYAACMLLFLPAAALVWSARYDHKPAPRAPVTAETLLA
                     GVRFIRARPVVLGAISLDLFAVLLGGATALLPIYARDILHAGPEGLGLLRGAPAVGAF
                     AMSVALARRTITRRVGHTLFLAVGLFGLTTLVFAVSHAFWLSMAALFVNGAADMVSVV
                     VRQTLVQLDTPDDMRGRVSAVNSIFIGASNQLGEFESGATAALLGPVGSVLLGGVGTL
                     VVVALWMKWFPQLVERESLVAAA"
     gene            complement(1364..1990)
                     /locus_tag="VF453_08635"
     CDS             complement(1364..1990)
                     /locus_tag="VF453_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7687759.1"
                     /translation="MDDQHLITDLAALEALFGPVGEASRRKQVATIPPVYRAVIEASP
                     FAVLATTGPGGLDASPRGDAPGFVQVIDERTLLLPERRGNNRIDSLRNVLADPRVGLL
                     FLVPGVDETLRVNGRARITVDPAVLGRCAVQGARPKCALWIEVDEVFFQCGRALIRSS
                     LWAPRAADAPRPVPTAGQMLAALTEGTVGGADYDAALPQRQRATLYAD"
     gene            complement(2026..>4673)
                     /locus_tag="VF453_08640"
     CDS             complement(2026..>4673)
                     /locus_tag="VF453_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX7687760.1"
                     /translation="AGSAPVQVLVADRQVRVEGGSLVRYLHFARRVNDASGLAALGQL
                     HVEFDPAYQKLVLHHVEIVRDGRRIDKLDPKAIHVLHRETQLERQVIDGRMTLSLVLA
                     DLRVGDIVEWDASEIGANPVFGGRFADTEGLVEGDGPIAHYRYRLLAPASRPLALRSG
                     AANLVPEQQTHGDERETVIDRRDVPRFAAEPGVPPVEWLKDQFDVSEFATWHDVAAWA
                     QPLFAKAMQASPAVDAEVEQLKAAGATPEARAIAALDFVQGQVRYFGTEVGMGTHQPA
                     PADTVLRQRFGDCKDKVSLLVTLLRRLDIDAVPVLVSIGLRDATAQREPTPLAFDHVI
                     VRATLDGKPYYLDATRNPQRGSLASRQAVGFGSGLVVADDTTALVALPAERDAAQAEV
                     VDTFSFPKLAEPGTLESVTTFHGDLADYIRATAAAAPQEKFDEGIEEPVLRSFDHLEP
                     QGHAVLAEVPGEDSVRVTTRYRVPQAWSFADTRTLTMPFRMAALAAPLAHPDTPAREQ
                     PFRFAHPGRYAYTLRMEFGEAIGLEPRGDRFDDDAPQYGLHVRYEGDPHGEQILADLQ
                     VNDDIVAPADWAAFRERLARLGPRLGGALHIPPLGPDDVAALRTEGQKLAQEMSGQHL
                     KPGNARIAVEARMQQLYLDKVLAGGRLPPKPRAQALMAKGIGLDHLGRPDDAKAAFDE
                     ALRLDPDNADIHEAAAVNAFGRGDDAQAIAQADASLKLAPSQVEPRYTRAWAEWFAGR
                     PQATRDGMLELLRSRTEVERSYGAIWLYLATRRLGGDAGAAAAAVAPYLPSSSHPAWP
                     WPALQVMLGRLDLKDAQAQAESEGKETGGQRDCELWFFAGEKALAEGDKARAKSLFED
                     SVGTGVVAFNEYQMAQRELKALPRP"
BASE COUNT          532 a         1705 c         1759 g          677 t
ORIGIN      
        1 ggcgcgcgcg ggctcgctcg cgggtgggcg tgcgctcgcc ggcggtgcga gggaggcctc
       61 gcgaaagtcc cgcgaccggc gccgggcacc gcgcccgtga gaccatcgcg gccctatgac
      121 cgatggctcg tcgctgcgcg cgtatccctc cttcctgcgt ttctgggcgg ggcgcctggc
      181 cggcacgatg gccaaccaga tgctgctggt ggccgtggcc tggcagatgt acgacgtcac
      241 cggcagcgcg ctgagcctgg gcctcgtcgg cctcgtgcag ttcgtgccgg cgctcgcgct
      301 ggtgctggtt tccggccacg tcgccgaccg ccacgaccgc cgccacatcg tcgcggccgc
      361 gttcctgctg cagatggccg cggcgctgct gctgctggtg gccacgcgcg agggccgcgt
      421 cgggcgcgag ctgatcctcg cggtgtcgct gctgctgggt gtcgcgcgct cgttccagat
      481 gacggcgcag caggcgctcg cgccgctgct ggtgccggcc ggcatgctcg cgcgcgcgct
      541 ggccttcagc tcctcgggca tgcaggccgc gatcatcggc gggcccgcgc tcgcgggcgt
      601 gttgtacggc ggcgtgctct tgcacgggtt cgcgctcacg ggcggtgccg acgtcgtcta
      661 cgcggcctgc atgctgctgt tcctgcctgc cgccgcgctc gtctggagcg cccgctacga
      721 ccacaagccg gcgccgcgcg cgccggtgac ggcggagacg ctgctggccg gcgtccgctt
      781 catccgcgcg cggccggtgg tgctgggcgc gatctcgctg gacctgttcg ccgtcctgct
      841 gggcggcgcg acggcgctgt tgccgatcta cgcgcgcgac atcctgcatg ccggccccga
      901 gggcctcggc ctgctgcgcg gggcgccggc ggtcggcgca ttcgcgatgt cggtcgcgct
      961 cgcgcgccgg acgatcacgc gccgcgtggg ccacacgctg ttcctcgccg tcggcctgtt
     1021 cgggctgacg accctcgtgt tcgcggtctc gcacgccttc tggctgtcga tggcggccct
     1081 gttcgtcaac ggcgcggccg acatggtcag cgtcgtcgtg cggcagacgc tggtgcagct
     1141 ggacacgccc gacgacatgc gcggccgcgt cagcgccgtc aactcgatct tcatcggcgc
     1201 gagcaaccag ctcggcgagt tcgagtccgg cgccaccgcc gcgctgctcg ggccggtcgg
     1261 ctcggtgctg ctgggcgggg tgggcaccct cgtcgtcgtg gcgctgtgga tgaagtggtt
     1321 cccgcagctc gtggagcgcg agagcctcgt cgcggcggcc tgatcaatcc gcgtagagcg
     1381 tcgcgcgctg gcgctgcggc agcgcggcgt cgtagtccgc tccgcccacc gtgccctcgg
     1441 tgagcgcggc cagcatctgc ccggcggtcg gcaccggccg cggcgcatcg gcggcccgcg
     1501 gcgcccacag gctggagcgt atcagcgcgc gcccgcactg gaagaacacc tcgtcgacct
     1561 cgatccagag cgcgcatttc ggccgcgcgc cctggacggc acagcgaccg agcaccgccg
     1621 gatcgacggt gatgcgcgcg cggccgttca cgcgcagcgt ctcgtcgacg cccggcacga
     1681 ggaacagcag cccgacgcgc ggatcggcca gcacgttgcg caggctgtcg atgcggttgt
     1741 tgccgcggcg ctcgggcagg agcagcgtgc gttcgtcgat cacctgcacg aagccgggcg
     1801 cgtcgccgcg cggcgaggcg tcgagcccgc ccgggcccgt cgtggccagc accgcgaacg
     1861 gcgacgcctc gatcaccgcg cggtagaccg gcgggatcgt cgcgacctgc ttgcggcgcg
     1921 aggcctcgcc cacggggccg aacagcgcct cgagggcggc gaggtccgtg atcaggtgct
     1981 ggtcgtccat ggcgtgtcct gggaggtggt ggggtccggg ggcgatcagg gccgggggag
     2041 cgccttgagc tcgcgctgcg ccatctggta ctcgttgaag gcgacgacgc ccgtgccgac
     2101 gctgtcctcg aacagcgact tcgcgcgcgc cttgtcgccc tccgccagcg ccttctcgcc
     2161 ggcgaagaac cagagctcgc agtcgcgctg gccgccggtc tccttgccct cggactcggc
     2221 ctgcgcctgc gcgtccttca agtcgaggcg gcccagcatc acctgcagcg ccggccacgg
     2281 ccaggccgga tgcgaagagc tcggcaggta gggcgcgacc gcggcggccg cggcgccggc
     2341 gtcgccgccc aggcggcgcg tcgccaggta cagccagatc gcgccgtagc tgcgctcgac
     2401 ctccgtgcgc gagcgcagga gctccagcat cccgtcgcgc gtggcctggg gacggcccgc
     2461 gaaccactcg gcccaggcgc gcgtgtagcg cggctcgacc tgcgagggcg cgagcttgag
     2521 cgacgcgtcg gcctgggcga tcgcctgcgc gtcgtcgccg cggccgaagg cgttgacggc
     2581 ggcggcctcg tggatgtcgg cgttgtccgg gtcgagccgg agcgcctcgt cgaacgcggc
     2641 cttcgcatcg tcgggccggc cgaggtggtc gagcccgatg cccttggcca tcagcgcctg
     2701 cgcgcgcggc ttgggcggca gccggccgcc tgccagcacc ttgtcgagat agagctgctg
     2761 catccgtgcc tcgaccgcga tgcgcgcgtt gcccggcttg aggtgctggc cggacatctc
     2821 ctgcgccagc ttctggcctt cggtgcgcag cgcggccacg tcgtccggcc cgagcggcgg
     2881 gatgtgcagc gcgccgccca ggcgcgggcc caggcgggcc aggcgttcgc ggaaggcggc
     2941 ccagtccgcc ggcgcgacga tgtcgtcgtt gacctgcagg tcggccagga tctgctcgcc
     3001 atgcggatcg ccctcgtagc gcacgtgcag gccgtactgc ggcgcgtcgt cgtcgaagcg
     3061 gtcgccgcgc ggctccaggc cgatcgcctc gccgaactcc atgcgcagcg tgtaggcgta
     3121 gcgccccgga tgcgcgaagc ggaacggctg ctcgcgcgcg ggcgtgtcgg gatgcgccag
     3181 cggcgcggcc agcgcggcca tgcggaacgg catcgtcagc gtgcgggtgt cggcgaagct
     3241 ccaggcctgc ggcacccggt agcgcgtcgt cacgcgcacg ctgtcctcgc cgggcacctc
     3301 ggcgagcacc gcgtggccct gcggctccag gtggtcgaag ctgcgcagca ccggctcctc
     3361 gatgccctcg tcgaacttct cctgcggcgc ggcggccgcg gtggcgcgga tgtagtcggc
     3421 caggtcgccg tggaaggtcg tgacggactc gagcgtcccc ggctccgcga gcttcgggaa
     3481 cgagaacgtg tcgacgacct cggcctgcgc cgcgtcgcgc tccgccggca gcgcgaccag
     3541 cgcggtcgtg tcgtcggcga ccacgaggcc ggagccgaag cccaccgcct gccgcgacgc
     3601 gagcgagccg cgctgcgggt tgcgcgtggc gtcgaggtag tacggcttgc cgtccagcgt
     3661 cgcgcgcacg atcacgtggt cgaaggcgag cggcgtcggc tcgcgctgcg ccgtcgcgtc
     3721 gcgcagcccg atggacacga gcaccggcac cgcgtcgatg tccagccgcc gcagcagcgt
     3781 gaccagcagc gagaccttgt ccttgcagtc gccgaagcgc tggcgcagca cggtgtccgc
     3841 gggcgcgggc tggtgcgtgc ccatgccgac ctcggtgccg aagtagcgca cctggccctg
     3901 gacgaagtcg agcgccgcga tcgcgcgcgc ctcgggcgtc gcgccggcgg ccttcagctg
     3961 ctcgacctcc gcgtcgacgg ccgggctcgc ctgcatcgcc ttggcgaaca gcggttgcgc
     4021 ccacgcggcg acgtcgtgcc aggtcgcgaa ctcgctgacg tcgaactggt ccttcagcca
     4081 ctcgaccggc ggcacgcccg gctcggcggc gaagcgcggc acgtcgcggc ggtcgatcac
     4141 ggtctcgcgc tcgtcgccat gcgtctgctg ctccggcacg aggttcgcgg cgcccgagcg
     4201 cagcgcgagc gggcgcgacg ccggcgccag cagacggtag cggtagtgcg cgatcgggcc
     4261 gtcgccctcg acgaggccct ccgtgtcggc gaagcgtccg ccgaagacgg ggttggcgcc
     4321 gatctccgag gcgtcccatt cgacgatgtc gccgacgcgc aggtcggcca gcaccagcga
     4381 cagcgtcatg cggccgtcga tcacctggcg ctcgagctgc gtctcgcggt gcagcacgtg
     4441 gatcgccttc gggtcgagct tgtcgatgcg acggccgtcg cgaacgatct cgacgtggtg
     4501 caggaccagc ttctggtagg ccggatcgaa ctcgacatgc agctgcccga gcgcggcgag
     4561 gccggaggcg tcgttgacgc ggcgcgcgaa gtgcaggtag cgcacgagcg agccgccctc
     4621 gacgcgtacc tggcggtcgg cgaccagcac ctgcaccggc gcggaacccg ccg
//
LOCUS       DASVLN010000351         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_179664, whole genome shotgun sequence.
ACCESSION   DASVLN010000351 DASVLN010000000
VERSION     DASVLN010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179664"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="VF453_08645"
     CDS             <1..743
                     /locus_tag="VF453_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX7687761.1"
                     /translation="QRAFQLATQLAHLEAGALIDELAHTGGFASPDARALARIGLASY
                     FAGALVLPYRRFLAAAEQLGYDIDLLSQRFGVSFETVCHRLSTLQRPDARGVPFFFVR
                     VDRAGNISKRQSATDFHFSHTGGTCPLWNVYEAFAHPGKVLTQVARMPDGRTYLWIAR
                     CVSRATGGYGSPERTFAIGLGCDVRHAHRLVYAKGLDLADSEVATPIGAGCRICDRRG
                     CPQRAFPALGKQLQVDENERPREPYASA"
     gene            complement(762..3374)
                     /locus_tag="VF453_08650"
     CDS             complement(762..3374)
                     /locus_tag="VF453_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687762.1"
                     /translation="MTTLRRLLASLGLAAACSLAHAAPASGPRWTTSLDEASGLPVVE
                     AGGVPAVKPSLAFWGPNWQFAYLKPELAPAGPGRYTARSEGKRLDVKLDEQAERRPDG
                     SWTLTFAMDGGSGTPGTSGGGLGFHFDLGTWRATMGEPELLPDNRGWRWGRDGGPQVE
                     VRFDHPLAAIMYEHPGDKSGLRAFFYTDRIPAGVTRYTATWTFKGIESRPMLAERLGG
                     PPPQDWPVDTLGNAGRIPDLSFLNRGDKPAGRHGELRRDGDRLVFADGTPARFWGTNV
                     VGYALYRTTKDDVRAQAKRLAAMGFNLVRLHQHDSPWVNPNIFGNPKTARDTQTLDPD
                     SLELLDWWVKCLKDEGIYVWLDLEVARVFKEGDHIDGFDEMRKGKDVAELRGFNYVNP
                     SIQAAMMRFDEAYLNHRNAFTHVANKDEPAIVALLLTNENDVTTHFGNALLPDKKVPV
                     NDKAYMDAAAEFAKANGVPAGKVWHAWEPGLPRIFLNDLEHRFDASEIEGLRRLGAKA
                     PIATTSTWGKSPVTALPALTTGDLIDVHVYGGVDPLSADPRTAPNLADWIGAAQVVGY
                     PLTVTEWNDEPFPTPDRHVVPLYLAATAAHQGWDALMQFAWTQEPTKNNRPSNWYMYN
                     DPSLMAMMPAAALLYRERHVAEAKSTYVLDLPADAFYGEPASAANSPALRSAMTRGRL
                     LVAMPETPALPWLKRIAPPAGATLLHDPHQSVLPPDATSATSDTGELTRDWGDGVYTI
                     STPRTRAALGWLGDRTFELGEVGIHMRTRNAAIAVQSLDERPIAEAGALLVSVGTRSQ
                     PSPGNRTPFVTEAASGTITVRAPAGMKLSAIDGDRETARPVAYRDGRYVIELDGKAPV
                     HWLVLRRGGDKAAE"
BASE COUNT          433 a         1285 c         1340 g          539 t
ORIGIN      
        1 gccagcgcgc gttccagctc gcgacgcagc tcgcgcatct cgaggccggc gcactgatcg
       61 acgagctcgc ccacacaggc ggcttcgcga gccccgacgc gcgcgcgctg gcgcgcatcg
      121 gcctggcgag ctacttcgcc ggcgcgctgg tgctgccgta ccggcgcttc ctcgccgccg
      181 ccgagcagct gggctacgac atcgacctgc tgtcgcagcg cttcggcgtc agcttcgaga
      241 ccgtgtgcca ccggctgtcg accctgcagc ggcccgacgc gcgcggcgtg ccgttcttct
      301 tcgtgcgcgt cgaccgcgcg ggcaacatct ccaagcgcca gtcggccacc gacttccact
      361 tctcgcatac gggggggacg tgtccgctgt ggaacgtcta cgaggccttc gcgcatccgg
      421 gcaaggtgct cacgcaggtc gcgcgcatgc ccgacggccg cacctacctg tggatcgcgc
      481 gctgcgtctc gcgcgcgacc ggcggctacg gctcgcccga acgcaccttc gcgatcggcc
      541 tgggctgcga cgtgcgccac gcccaccggc tcgtctacgc gaagggcctg gacctcgccg
      601 actccgaggt cgcgaccccg atcggcgccg gctgccgcat ctgcgaccgc cgcggctgcc
      661 cgcagcgcgc gttcccggcg ctgggcaagc agttgcaggt cgacgagaac gagcgcccgc
      721 gcgagccgta cgcgagcgcg taggccgcgg gggcggggcg gctactctgc ggccttgtcg
      781 ccgccgcgcc gcagcacgag ccagtgcacc ggcgccttgc cgtcgagctc gatgacgtag
      841 cgcccgtcgc ggtacgccac cggccgcgcg gtctcccggt cgccgtcgat cgcagacagc
      901 ttcatgccgg ccggcgcgcg cacggtgatc gtgccggacg ccgcctcggt gacgaagggc
      961 gtgcggttgc cgggcgaggg ctgcgagcgc gtgccgaccg acacgagcag cgcgcccgcc
     1021 tcagcgatcg ggcgctcgtc caggctctgc accgcgatcg ccgcgttgcg cgtgcgcatg
     1081 tggatgccga cctcgccgag ctcgaacgtg cggtcgccga gccagcccag cgcggcgcgc
     1141 gtgcgcggcg tggagatcgt gtacacgccg tcgccccagt cgcgcgtgag ctcgccggtg
     1201 tcgctcgtcg cgctggtcgc gtcgggcggc agcaccgact ggtgcggatc gtgcagcagc
     1261 gtcgcgccgg cgggcggcgc gatgcgcttg agccagggca gggcgggcgt ctcgggcatc
     1321 gccacgagca gccgcccgcg cgtcatcgcg ctgcgcagcg cgggcgagtt cgcggccgat
     1381 gccggctcgc cgtagaacgc gtccgccggc aggtcgagca cgtaggtcga cttcgcctcg
     1441 gcgacatggc gctcgcggta cagcagcgcg gccgcgggca tcatcgccat cagcgacggg
     1501 tcgttgtaca tgtaccagtt cgacgggcgg ttgttcttcg tcggctcctg cgtccatgcg
     1561 aactgcatca gcgcgtccca gccctggtgc gccgcggtgg cggccaggta cagcggcacg
     1621 acgtggcggt cgggggtggg gaacggctcg tcgttccatt ccgtgacggt cagcggatag
     1681 ccgacgacct gggcggcgcc gatccagtcc gcgaggttgg gcgcggtgcg cggatccgcc
     1741 gacagcgggt cgacgccgcc gtagacgtgc acgtcgatca ggtcgccggt ggtcagcgcg
     1801 ggcagcgcgg tgaccgggct cttgccccag gtgctcgtcg tggcgatcgg cgccttcgcg
     1861 ccgaggcggc gcagcccctc gatctcgctc gcgtcgaagc ggtgctcgag gtcgttgagg
     1921 aagatccgcg gcaggcccgg ctcccaggcg tgccagacct tgccggccgg cacgccgttg
     1981 gccttggcga actccgcggc ggcgtccatg taggccttgt cgttgaccgg caccttcttg
     2041 tcggggagca gcgcgttgcc gaagtgcgtc gtgacgtcgt tctcgttggt cagcagcagc
     2101 gcgacgatcg cgggctcgtc cttgttggcg acgtgcgtga acgcgttgcg gtggttcagg
     2161 tacgcctcgt cgaagcgcat catcgccgcc tggatcgacg ggttgacgta gttgaagccg
     2221 cgcagctccg cgacgtcctt gcccttgcgc atctcgtcga agccgtcgat gtggtcgccc
     2281 tccttgaaga cgcgcgcgac ctcgaggtcc agccagacgt agatgccctc gtccttcagg
     2341 cacttgaccc accagtccag cagctccagc gagtcgggat cgagcgtctg ggtgtcgcgc
     2401 gcggtcttgg ggttgccgaa gatgttcggg ttcacccacg gcgagtcgtg ctggtgcagg
     2461 cgcacgaggt tgaagcccat cgccgcgagc cgcttggcct gcgcgcgcac gtcgtccttg
     2521 gtcgtcctgt acaacgcgta gcccacgacg ttggtgcccc agaagcgcgc gggcgtgccg
     2581 tcggcgaaga cgaggcggtc gccgtcgcgc cgcagctcgc cgtggcggcc ggcgggcttg
     2641 tcgccgcgat tcaggaagct caggtcgggg atgcggccgg cgttgcccag cgtgtcgacg
     2701 ggccagtcct ggggcggcgg gccgcccagg cgctcggcga gcatcggccg cgactcgatg
     2761 cccttgaacg tccaggtggc cgtgtagcgg gtcacgccgg cgggaatcct gtcggtgtag
     2821 aagaacgcgc gcaggccgct cttgtcgccg gggtgctcgt acatgatcgc ggccagcgga
     2881 tggtcgaagc gcacctcgac ctgcggcccg ccgtcgcggc cccagcgcca gccgcggttg
     2941 tcgggcagca gctcgggctc gcccatcgtc gcgcgccagg tgccgaggtc gaagtggaag
     3001 cccaggccgc cgccgctcgt tccgggggtg cccgagccgc cgtccatcgc gaacgtgagc
     3061 gtccagctcc cgtcgggacg gcgctcggcc tgctcgtcga gcttgacgtc cagccgcttg
     3121 ccttcgctgc gcgccgtgta gcgcccgggg cccgcgggcg cgagctccgg cttgaggtag
     3181 gcgaactgcc agttcgggcc ccagaacgcg agcgagggct tgaccgccgg cacgccgccg
     3241 gcctcgacca ccggcaggcc gctcgcctcg tcgaggctgg tcgtccagcg cgggccggag
     3301 gcgggcgccg cgtgggcgag cgagcaggcg gcggcgaggc cgagcgaggc gaggagacgg
     3361 cgaagcgtgg tcatcgatgt cctggtggcg acgggcgtcc gcatgggatc cgggcgtgcc
     3421 cgtggggttc ccgcctcggc aagcgtcgtg cgaggccgcg cgcggcttca ggttcggaaa
     3481 tagcgttgcg ttccgcatgc tacccgcgcc gcgccgcgcc gcgacccggc cggccgccgt
     3541 caggccgcgt cgtcggcgag cagcgcgcgc cactcggggc cgggcgcgca ctccagc
//
LOCUS       DASVLN010000352         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_393706, whole genome shotgun sequence.
ACCESSION   DASVLN010000352 DASVLN010000000
VERSION     DASVLN010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393706"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VF453_08655"
     CDS             complement(<1..300)
                     /locus_tag="VF453_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein"
                     /protein_id="HEX7687763.1"
                     /translation="MTMDAAPVTAGPLGLMRLVLRFNLHPEASLHPSWLPPDWPVHAR
                     MRLARLGPQAQAVLRDVLARLGVVDVQADYAFDSPVKRLLLLDAGSLRRLAIYASL"
     gene            complement(434..1213)
                     /gene="sctJ"
                     /locus_tag="VF453_08660"
     CDS             complement(434..1213)
                     /gene="sctJ"
                     /locus_tag="VF453_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HEX7687764.1"
                     /translation="MTTRTFLPRIAACAALALLLAGCKVELYSGLNETEANEVVAALA
                     DGRIDARKERVDGGDWQVQVDEDRVGPALDLLRAQGLPEQHYASMGDMFQKQGLVSTP
                     AEERMRYIFAVSQELSHTLRSIDGVVEARVHVVIPQDDPLSDKVRPSSAAVFIKHRPD
                     VDLRLLAPAVKDLVAHSIEGLTPDQVALTLLPAPRAAPAATGFAPVDSAREVLGMSQR
                     AVVTLLVLLIVAALALMALPTLLARRGLDLRTWLQREVLRR"
     gene            complement(1218..1604)
                     /locus_tag="VF453_08665"
     CDS             complement(1218..1604)
                     /locus_tag="VF453_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687765.1"
                     /translation="MTDPIAVLAASAPQAAAATTPPPAHLEPADTRMFATLMQGQPAL
                     DPAATPTPGDIAHSFAAEFGNTRSFDEIRTSMLKAYDPRDPIKSMFTVANNGMEAQML
                     FSKLHIATSLASSATSLFGSLLKNQG"
BASE COUNT          232 a          597 c          647 g          223 t
ORIGIN      
        1 caggctggcg tagatcgcga gccgccgcag cgagccggcg tccagcagca gcaagcgctt
       61 gaccggcgaa tcgaacgcgt agtcggcctg cacatcgacg acgcccaggc gcgcgagcac
      121 gtcgcgcagc acggcctgcg cctgcggccc gagccgcgcc agccgcatcc gcgcgtgcac
      181 cggccagtcc ggcggcagcc agctcgggtg caggctcgcc tccggatgca ggttgaagcg
      241 cagcaccagg cgcatcaggc cgagcgggcc ggccgtcacc ggcgcggcat ccatggtcat
      301 tcgaagggct ccgtcaagtc gatctcgcat ccgacgcgtg gcagggccgc tcccggccgg
      361 ccagcgcccg gccgcccgaa gcaggccggc tcagcccctt gggggcggcg agcggacgcg
      421 agcggggtcg tcatcaccgg cgcaggacct cgcgctgcag ccaggtccgc aggtcgagcc
      481 cgcgccgcgc cagcagcgtc ggcagtgcca tcagggcgag cgcggcgacg atcagcagca
      541 ccagcagcgt gaccaccgcg cgctgcgaca tgcccagcac ctcgcgcgcc gagtcgaccg
      601 gcgcgaagcc cgtcgctgcc ggcgcggcgc gcggcgccgg cagcagcgtc agcgcgacct
      661 ggtcgggcgt gaggccctcg atgctgtggg cgaccagatc cttgaccgcg ggcgcgagca
      721 gccgcaggtc gacgtcgggg cggtgcttga tgaagacggc cgccgacgag ggacgcacct
      781 tgtcgctcag cgggtcgtcc tgcgggatga ccacgtgcac ccgcgcctcg acgacgccgt
      841 cgatcgagcg cagcgtgtgc gacagctcct gcgagaccgc gaagatgtag cgcatgcgct
      901 cctcggcggg cgtcgagacc aggccctgct tctggaacat gtcgcccatg ctcgcgtagt
      961 gctgctcggg caggccctgc gcgcgcagca ggtcgagggc cgggccgacg cggtcctcgt
     1021 cgacctgcac ctgccagtcg ccgccgtcca cgcgctcctt gcgcgcgtcg atgcggccgt
     1081 cggcgagcgc ggcgacgacc tcgttggcct cggtctcgtt gagccccgag tagagctcga
     1141 ccttgcagcc ggccagcagc agcgcgagcg cggcgcaggc ggcgatccgc gggaggaagg
     1201 tgcgggtggt catcgcatca gccctggttc ttcagcaggc tgccgaacag gctggtcgcc
     1261 gagctcgcca ggctggtggc gatgtgcagc ttggagaaca gcatctgcgc ctccatgccg
     1321 ttgttcgcga cggtgaacat gctcttgatc gggtctcgcg ggtcgtaggc cttgagcatg
     1381 ctcgtccgga tctcgtcgaa cgagcgcgtg ttgccgaact cggcggcgaa cgaatgcgcg
     1441 atgtcgcccg gcgtgggcgt cgcggcggga tcgagcgcgg gctggccctg catcagggtc
     1501 gcgaacatgc gcgtgtcggc cggctcgaga tgggccgggg gcggggtggt cgcggcggcg
     1561 gcctgcggcg cgctcgcggc gaggacggcg atgggatcgg tcatggcggg ccctttcctt
     1621 cgaagatgcg gacacgggcg aggccgcgcg ccagggagga tgcgcgcgcg ggctcgcccg
     1681 tgctgcgcgg cgccctcgc
//
LOCUS       DASVLN010000353         3694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_179713, whole genome shotgun sequence.
ACCESSION   DASVLN010000353 DASVLN010000000
VERSION     DASVLN010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179713"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1994
                     /locus_tag="VF453_08670"
     CDS             <1..1994
                     /locus_tag="VF453_08670"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX7687766.1"
                     /translation="SSPPTELAAASAAGAGEAEPHLLAAAFDALSSDPPAAGTAMTQP
                     VVVGRLVLVHDLSFIEKRSQDTRRYLVGLIAILGFVMALITMVVAQLSWRGWVQGVRA
                     LLRGEGIISPMDGSGSVAPPPELEPFASDLRERLRDLEDEYRRMQGGPEASWNAERLR
                     RLLMTQLRGDQVIVVSNREPYIHQHVKGSEEIVVKRPASGLVTAVEPVMRACSGTWIA
                     HGSGDADREVVDANDRVAVPPGHDDYQLRRLWLTPEEEQGYYFGFANEGLWPLCHVAH
                     VRPVFRESDWEAYRTVNQRFADAVVAEARSEDPIVLVQDYHFALLPAMVREKLPNATI
                     ITFWHIPWPNPESFGICPWRREILQGMLGSTILGFHTRFHCKNFIETVDRFLEARIEH
                     EHSTISFKEDETLVESYPISIEWPSDETIASWPSIEECRHRVRERLGLPATVTLAVGI
                     DRFDYTKGILERLNAVERLLEKHPEWIERFVFVQVAAPTRSSLDEYRIFQEQVERVTT
                     RINERFGRPGYQPVHLLAQHHDTAAVNELFRAADACVVTSLHDGMNLVCKEFVAARDD
                     EQGVLILSRFAGAAREMTEALIVNPYHVEETADALHRAATMPPAEQRARMSSMRSTVH
                     EFNVFRWAGRMLTDAGRWRLKARVDARVRRFRHEGQRGL"
     gene            2227..3222
                     /gene="otsB"
                     /locus_tag="VF453_08675"
     CDS             2227..3222
                     /gene="otsB"
                     /locus_tag="VF453_08675"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX7687767.1"
                     /translation="MSKKATGDWTPSAPPFAPISPAAPPRGHASDDPAHDDPPSGEHP
                     DTAADFPHGIEDDVQPLQSGVDTGAGACVGAQLRHLSSPEGQAALVALMRGSVLFAFD
                     FDGTLAPIRPRPGDVHVSATLALRLEKLARLRPVAIVTGRRIDDVRGRLGFTPTWIVG
                     NHGAEDDGDPDAALRAIQALDPLRRRLEARADGLQDAGVAIEDKGQSLALHFRTAPDR
                     DAARALIDEVLAGFTPQLHVFGGKLVVNAVAADAPDKAVAVRRLLARSGAQNAFFAGD
                     DVNDEPVFAAAQPGWLTVRIGCDRRGTSAARFCIDGPHEMAVVLDRLLAQVDGSA"
     gene            complement(3391..>3694)
                     /locus_tag="VF453_08680"
     CDS             complement(3391..>3694)
                     /locus_tag="VF453_08680"
                     /inference="COORDINATES: protein motif:HMM:NF042415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HEX7687768.1"
                     /translation="ARAPGNRLAAVQALRASFYRGDPRALDVLLPEATHRQGDVETST
                     WDFPGDAAMPVWIACVYGGDEVLATRPLPAGTRACTARLRVTRMGDPAGLLAVSCR"
BASE COUNT          522 a         1357 c         1309 g          506 t
ORIGIN      
        1 cgtcgtcccc gcccacggaa ctggccgccg cctcggccgc cggcgcgggc gaggcagagc
       61 cgcacctgtt ggccgccgcg ttcgatgcgc tgtccagcga cccgcctgcc gcgggcacgg
      121 cgatgacgca gccggtcgtc gtcgggcggc tggtcctcgt ccacgacctg agcttcatcg
      181 agaagcgcag ccaggacacg cgacgctacc tcgtcggcct catcgccatc ctgggtttcg
      241 tgatggcgct gatcaccatg gtggtggcgc aactgagctg gcgcgggtgg gtgcagggcg
      301 tgcgggcgct gctgcgcggc gagggcatca tcagccccat ggatggcagc gggagcgtgg
      361 cccctccgcc ggagctcgaa cccttcgcgt ccgacctgag ggagcggctg cgcgacctcg
      421 aggacgaata tcgccgcatg cagggcggcc ccgaggcgag ctggaacgcc gagcgcctgc
      481 gccgcctgct catgacgcag ctgcggggcg accaggtgat cgtcgtctcc aaccgcgagc
      541 cgtacatcca tcagcacgtc aagggcagcg aggagatcgt cgtgaagcgg ccggcgagcg
      601 gactggtcac ggccgtcgag ccggtgatgc gcgcatgctc gggcacctgg atcgcgcacg
      661 gcagcggcga cgcggatcgc gaggtcgtgg acgccaacga ccgcgtcgcc gtgccgccgg
      721 ggcacgacga ctaccagctg cgccgcctct ggctcacgcc cgaggaggag caaggctact
      781 acttcggctt cgccaacgaa ggcctgtggc cgctgtgcca cgtggcccac gtgcgtccgg
      841 tgttccgaga gtccgactgg gaggcctacc gcaccgtcaa ccagcgcttc gccgacgcgg
      901 tcgtcgctga ggcacgcagc gaggatccca tcgtcctcgt gcaggactac cacttcgcgc
      961 tgctgccggc gatggtgcgc gagaagctgc cgaacgcgac catcatcacg ttctggcaca
     1021 tcccctggcc gaacccggag tccttcggca tctgcccgtg gcggcgcgag atcctgcaag
     1081 gcatgctggg ctcgacgatt ctcggcttcc acacgcgctt ccactgcaag aacttcatcg
     1141 agaccgtgga tcgcttcctc gaggcgcgca tcgagcacga gcattcgacg atctcgttca
     1201 aggaggacga gacgctggtc gagtcctatc cgatctcgat cgagtggccg agcgacgaga
     1261 cgatcgcctc ctggccgtcg atcgaggaat gccgccaccg ggtgcgcgag cgcctcgggc
     1321 tgcccgcgac cgtcacgctg gcggtgggca tcgaccgctt cgactacacg aagggcatcc
     1381 tcgagcgcct gaacgcggtg gagcggctgc tcgagaagca tcccgagtgg atcgagcgct
     1441 tcgtgttcgt ccaggtcgcc gcgccgacgc ggagctcgct cgacgaatac cgcatcttcc
     1501 aggagcaggt cgagcgcgtg acgacgcgca tcaacgagcg cttcggccgc ccgggctacc
     1561 agccggtgca cctgctcgcg cagcaccacg acacggccgc cgtcaacgag ttgttccgcg
     1621 ccgccgatgc ctgtgtcgtc accagcctgc acgacggcat gaacctggtc tgcaaggagt
     1681 tcgtggccgc gcgcgatgac gaacagggcg tgctgatcct cagccgcttc gccggcgccg
     1741 cgcgcgagat gaccgaggcg ctgatcgtca atccatacca cgtcgaggag acggccgacg
     1801 cgctgcaccg cgcggcgacg atgccgccgg ccgaacagcg ggcgcgcatg tccagcatgc
     1861 gcagtacggt gcacgagttc aacgtcttcc gctgggccgg ccgcatgctc accgacgccg
     1921 ggcgctggcg cctgaaggct cgcgtcgacg cgcgggtgcg ccgcttccgg cacgaaggac
     1981 agagagggct ctgacatgag ggagcacacc cgacccaccg tggacggcgc cggcgatcgt
     2041 ccgcccgatg ccgacaccgg cgcaccgacc cctcgcatgg gcgtcgatcc gttgcctccg
     2101 cgatcgcgcg agccgtcctg gcgcggcgag cctgcggcgg acgtacgcgc ctccggggag
     2161 ccatcggccg gccagtcgtc gccaggcggc ctgccgctcg agcagggctc gtccggcgac
     2221 ccttcgatgt cgaagaaagc aaccggcgac tggacgccct ccgcgccgcc cttcgcgccg
     2281 atttccccgg ccgcgccgcc ccgaggccat gcgtccgacg accccgcgca cgacgacccg
     2341 ccctccggcg agcaccccga cacggccgcc gacttccccc atggcatcga ggacgacgtg
     2401 cagccgctgc agtccggcgt cgacaccggc gccggcgcgt gcgtgggggc gcagttgcgg
     2461 cacctgtcca gccccgaggg gcaggcggcg ctcgtggcgc tgatgcgcgg ctccgtgctg
     2521 ttcgcgttcg acttcgacgg cacgctggcg ccgatccgcc cgcgccccgg cgacgtgcac
     2581 gtatcggcga cgctcgcgct gcggctcgag aagctcgccc ggctgcggcc ggtggccatc
     2641 gtcacgggac ggcggatcga cgacgtgcgc ggccggctcg gcttcacgcc gacctggatc
     2701 gtgggcaacc acggcgccga ggacgacggc gatccggatg ccgctttgcg cgccatccag
     2761 gcgctggatc cgctgcggcg gcggctcgag gcacgcgccg acgggctcca ggacgccggc
     2821 gtcgcgatcg aggacaaggg ccagtcgctc gccctgcatt tccgcaccgc tccggatcgc
     2881 gacgccgccc gcgcgctgat cgacgaggtg ctggccggct tcacgccgca gctgcacgtc
     2941 ttcggcggca agctggtggt caatgccgtc gccgccgacg cgcccgacaa ggcggtggcc
     3001 gtgcggcggc tgctcgcccg ctcgggcgcg cagaacgcgt tcttcgctgg cgacgacgtc
     3061 aacgacgagc ccgtcttcgc cgccgcgcag cccggctggc tcacggtgcg catcggctgc
     3121 gaccgccgcg ggacctccgc tgcgcgcttc tgcatcgacg gcccgcacga gatggcggtc
     3181 gtgctcgacc gcctgctggc ccaggtcgac ggcagcgcct gagccgcgac gcgagccgtc
     3241 gcgctggcga cggcaatggt gccgcgcgac cgccgttcgc ggcgggatgg cgtgcgtctg
     3301 gaccgcgccg acgcgcaggc gctcgcgcgc gtgtgcctga acatgcgcct gcgtgcgcat
     3361 gcgtcgtgct ggcgacccgt catcgcgccc tcagcggcag ctcacggcca gcaggccggc
     3421 gggatcgccc atccgcgtga cgcgcaggcg agccgtgcag gcacgcgtcc ccgcgggcag
     3481 cggccgtgtc gcgagcactt cgtcgccgcc gtagacgcag gcgatccaga ccggcatcgc
     3541 cgcgtcgccg gggaagtccc aggtcgaggt ctcgacgtcg ccttggcgat gcgtcgcctc
     3601 gggcagcagc acgtcgagcg cgcgcgggtc gccgcggtag aacgaggcgc gcagcgcctg
     3661 gacggcggcg agcctgttgc ccggcgcgcg cgct
//
LOCUS       DASVLN010000354         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_340288, whole genome shotgun sequence.
ACCESSION   DASVLN010000354 DASVLN010000000
VERSION     DASVLN010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340288"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="VF453_08685"
     CDS             complement(<1..892)
                     /locus_tag="VF453_08685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7687769.1"
                     /translation="MSLTTLHPMLNIAVKAARAAGSVINRASLDIEKVAVQTKSANDF
                     VTEVDEQAERVIIETILQAYPGHGILAEESGRSAGAKDSEFVWIIDPLDGTTNFIHGF
                     PVYAVSIALAYRGVVQQAVVYDPNRNDLFVASRGRGAYLNDRRLRVSRRLRLADSLIG
                     TGFPFRKGDNLKRYMKMFEEVSVECAGLRRPGAAALDLCYVAAGYLDGFFETGLSPWD
                     VAAGSLIITEAGGLIGNFTGESDFLYQREVVCGNPKVYGQLVGILAPYTRVIKDEPAA
                     TADAAEAAPAPDATDAFLADA"
     gene            1125..1892
                     /locus_tag="VF453_08690"
     CDS             1125..1892
                     /locus_tag="VF453_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7687770.1"
                     /translation="MTDSRPASSDPTRFVLVDTSHPGNVGAAARAMKVMGFSELVLVA
                     PRFADVLSHPEAIAFASGATDVLERARVVATLEEALAGATFACATAMTPRDFGPPTRP
                     PRDCFAELAATDHVAAFVFGGERFGLSNEHVYACHVCLSIPTQPEYGSLNLAQAVQIL
                     AYDWRQALGGFPVVERTRAPAKADAAAVHGLLAHWREALVAIGFLDPAVPRKLMARLH
                     QLVNRAQPTPEEVHILRGIARQVQARAGRADDAAPPA"
     gene            2021..2797
                     /gene="cysE"
                     /locus_tag="VF453_08695"
     CDS             2021..2797
                     /gene="cysE"
                     /locus_tag="VF453_08695"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX7687771.1"
                     /translation="MFARLREDIACILERDPAARSRLEVLLCYPGLHALVWHRGAHWC
                     WRRGLRVLGRFLSHVGRLLTGIEIHPGATIGRRVFIDHGMGVVIGEMAEVHDECTIYQ
                     GVTLGGTSLAKGAKRHPTLERGVIVGANACVLGGFTVGAGARVGSGAVVTKPVPPGAT
                     AVGNPARIIAAADDAAREATAARLGFSAYGVTQGDDPVAQAMKGLIDNASSHEHQIAL
                     LWQAIERLAADNARLARDCVPGDAQVDEHFDAEGLSRLVK"
     gene            2807..>2983
                     /locus_tag="VF453_08700"
     CDS             2807..>2983
                     /locus_tag="VF453_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7687772.1"
                     /translation="MRCVLDHLVVTAPSLDEGAAWIERHLGVAPRPGGRHPRMGTHNR
                     VLRLGAAAYLEVIAI"
BASE COUNT          394 a         1056 c         1118 g          415 t
ORIGIN      
        1 cggcgtcggc gaggaaggcg tcggtcgcgt ccggggcggg cgcggcctcg gcggcatcgg
       61 ccgtcgcggc gggctcgtcc ttgatcacgc gcgtgtaggg ggcgaggatc cccaccagct
      121 ggccgtagac cttgggattg ccgcagacga cctcgcgctg gtacaggaag tccgactcgc
      181 cggtgaaatt gccgatcagg ccgccggcct cggtgatgat cagcgagccc gcagcgacgt
      241 cccaggggct gaggccggtc tcgaagaagc catcgaggta gccggcggcg acgtagcaga
      301 ggtccagcgc ggccgcgccg ggtcggcgca ggcccgcgca ctcgacggag acctcctcga
      361 acatcttcat gtagcgcttg aggttgtcgc ccttgcggaa cgggaagccg gtgccgatca
      421 gcgagtctgc gagccgcagg cggcgcgaga cgcgcaggcg gcggtcgttg aggtaggcgc
      481 cgcggccgcg cgaggcgacg aacaggtcgt tgcggttcgg gtcgtagacg acggcctgct
      541 gcacgacgcc gcggtacgcg agcgcgatcg agaccgcgta gaccgggaag ccgtggatga
      601 agttggtggt gccgtccagc ggatcgatga tccagacgaa ctcgctgtcc ttggcgcccg
      661 ccgagcggcc cgactcctcg gccaggatgc cgtggcccgg gtaggcctgc aggatggtct
      721 cgatgatcac gcgttcggcc tgctcgtcga cctcggtcac gaagtcgttg gcgctcttcg
      781 tctgcacggc gaccttctcg atgtcgagcg aggcccggtt gatcacggag ccggccgcgc
      841 gcgccgcctt gacggcaatg ttgagcatcg ggtggagcgt ggtcagcgac atgggtctgg
      901 ttccaggatg gacgacacgg cgtccgcgcg ggcaggcgga cgtcgacggt ggacgaaggg
      961 cggcaggcga cgaaagagcg gcgcgcgaag gtcgcgggtg gcggacgcgg cagacgcgtt
     1021 gcggcgacaa tgcgggtgaa ccctgaattc tagccgcgcg aggctcgcgt gtcacctcga
     1081 ccgcggcggc gcgcgcccga cgacgctttc ccacggtgca ttccttgaca gattcccggc
     1141 ccgcgagttc cgacccgacg cgcttcgtgc tcgtcgacac cagccatccg ggcaacgtcg
     1201 gcgccgcggc gcgggcgatg aaggtgatgg gcttctcgga gctggtgctg gtcgcgccgc
     1261 gctttgccga cgtgctgtcg catccggagg cgatcgcgtt cgcgtccggc gcgaccgacg
     1321 tgctcgaacg cgcgcgcgtc gtcgcgacgc tggaggaggc gctcgccggg gcgaccttcg
     1381 cctgcgcgac cgcgatgacg ccgcgcgact tcggcccgcc gacgcgcccc ccgcgcgact
     1441 gcttcgccga gctcgcggcc accgaccacg tcgccgcctt cgtcttcggc ggcgagcgct
     1501 tcggcctgtc caacgagcac gtctacgcct gccacgtctg cctgagcatc cccacgcagc
     1561 ccgagtacgg ctcgctcaac ctcgcgcagg cggtgcagat cctcgcctac gactggcgcc
     1621 aggcgctggg cggcttcccg gtcgtcgagc gcacgcgcgc gcccgcgaag gccgacgccg
     1681 cggcggtgca cggcctgctg gcccactggc gcgaggcgct ggtggcgatc ggcttcctcg
     1741 atcccgcggt accgcgcaag ctcatggccc ggctgcacca gctcgtcaac cgcgcgcagc
     1801 cgacgcccga ggaagtgcac atcctgcgcg gcatcgcgcg ccaggtgcag gcgcgggcgg
     1861 ggcgggccga cgacgcggcg ccgcctgcct gatcgcccgg ggacgcgcac gcctgcgcgc
     1921 ggacggcccg acggcgaccg gggcttgtcc gccggagcgc ggccgtgacg ggggcgcccg
     1981 ctacactcgc cgctcccgtc gacttccccc gccgcccact atgttcgccc gcctgcgcga
     2041 agacatcgcc tgcatcctgg agcgcgaccc ggccgcccgg tcgcgcctcg aggtgctgct
     2101 gtgttatccg ggcctgcatg cgctggtgtg gcaccgcggt gcgcactggt gctggcggcg
     2161 cggactgcgc gtgctcgggc gcttcctgtc gcacgtcggg cgcctgctca ccggcatcga
     2221 gatccacccc ggcgcgacga tcggccggcg cgtgttcatc gaccacggca tgggcgtcgt
     2281 catcggcgag atggccgagg tgcacgacga gtgcacgatc taccagggcg tgacgctggg
     2341 cggcacctcg ctcgccaagg gcgcgaagcg gcatccgacg ctggaacgcg gcgtgatcgt
     2401 cggcgccaac gcctgcgtgc tgggcggctt cacggtcggt gccggcgcgc gcgtgggctc
     2461 gggcgcggtc gtgaccaagc cggtgcctcc gggcgcgacg gcggtcggca atccggcgcg
     2521 catcatcgcc gccgcggacg acgcggcccg cgaggcgacg gccgcgcggc tgggcttctc
     2581 ggcctacggc gtgacgcagg gcgacgaccc ggtggcgcag gcgatgaagg gcctgatcga
     2641 caacgcgtcc tcgcacgaac accagatcgc gctgctctgg caggcgatcg agcgcctggc
     2701 cgccgacaac gcccgcctcg cgcgcgattg cgtcccgggc gacgcccagg tcgacgagca
     2761 cttcgatgcc gagggcctga gccggctcgt gaagtaggag ccgacgatgc gctgcgtcct
     2821 ggatcacctc gtcgtcaccg cgccgtcgct ggacgagggc gccgcatgga tcgagcggca
     2881 cctgggcgtg gcgccgcgcc cgggcggccg ccatccgcgc atgggcacgc acaaccgcgt
     2941 gctgcggctg ggcgcggcgg cctacctcga ggtgatcgcg atc
//
LOCUS       DASVLN010000355         4349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_126274, whole genome shotgun sequence.
ACCESSION   DASVLN010000355 DASVLN010000000
VERSION     DASVLN010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126274"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VF453_08705"
     CDS             <1..273
                     /locus_tag="VF453_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687773.1"
                     /translation="LPASAPASAPPPGLDLTVTRDAIRAAAAAQPSVAQMAQLQTHVG
                     PATSDERLSQGVAAAVKPDCGTAAAGAGLFALPVLIYAEAAGKCNN"
     gene            261..746
                     /locus_tag="VF453_08710"
     CDS             261..746
                     /locus_tag="VF453_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687774.1"
                     /translation="MQQLTPPIDASARAPSRYAAVQPAPRADVAADTPALPAFTSFAD
                     VRDMFTFVAFGAPRFDYVRRLPGSPRALTLESAFDALRRGVEPFERRSKRGRQRALYA
                     QALAELDAALAHYRAGRRTYGRLTLELARCRFVEAGSTQAAPRARADAEALAARLAAL
                     T"
     gene            complement(749..2497)
                     /locus_tag="VF453_08715"
     CDS             complement(749..2497)
                     /locus_tag="VF453_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7687775.1"
                     /translation="MTATPADPEPSQDFHAALHAGLRWHVPERMNIAELCVHRWARET
                     PDAVAIVHDHGDAADAPPPTAWRYARLQAESRRLSNALAALGVEPGDRVAVVMPQRPQ
                     MAIALMAVLQMGAIAVPLSTLFGQEALQSRLADSGAVVALVDDGSTAAIAALRPACPA
                     LRTVIGTGLVAADCDLAWSVALAGASTEFETADTRADDAAFLIYTSGTTGPAKGARIP
                     HRALIGNLSGFVASQNWCEPGGPFWSPADWTWTGGLWDALLPTLYLGRTIVAFGGRFD
                     ADFALRLMARHGVTHTFLFPTALKAMMRVAPDPRTSHPGVKLRAIMSAGEAVGEGVFA
                     YCRDKLGVVVNEMFGQTEMNYVVGNCGRWVDRDGRAHPGWPPRPGSMGRPYPGHRVAV
                     IDDDGEPCAPGVEGDVAVHARDVHGDRDPVFFLGYWNNDRATRAKFTGKPFESWCRTG
                     DRAVMDEDGYLWYQGRSDDQFKSSGYRIGPTEIEDCLARHPAVAQVAVVPKPDPDRGA
                     VVKAFVILAAGHAATPELVEALQQLVRSRLAPYETPKDIEFVDALPMTTTGKLQRNVL
                     RRLEEDRAAQRAAGAG"
     gene            complement(2561..3058)
                     /locus_tag="VF453_08720"
     CDS             complement(2561..3058)
                     /locus_tag="VF453_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7687776.1"
                     /translation="MTATPTSDIRFATRADLPAIVGLIRELAVYEKLEHLATATPERL
                     EPHLFGERPAAECLVAEQGGAVVAFALFFHNFSTFLCRPGLYLEDLYVQPAARGTGLG
                     RRLLQRLAAIAVERGCGRFEWSVLDWNVDAQAFYARMGATVMPDWRIVRATGDALAAM
                     ASAAA"
     gene            3183..4115
                     /locus_tag="VF453_08725"
     CDS             3183..4115
                     /locus_tag="VF453_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7687777.1"
                     /translation="MSAPTVYAPLRPARTASHAIRTIAMQVHEWGDASLATPERPTLV
                     LMHGWMDVGASFQFVVDELARLEGEARHVIAADWRGFGGSTSGEVDAFWFQDYLGDLE
                     ALLDAFAPGRAVDLAGHSMGGNVVMTYAGVRPARVRRLVNLEGFGLPDSKPEHAPARL
                     AKWLDELKTPQSLRPYASLGEVAERLRRNNPRLSPERAAWLAPHWSRRGADGLWHVLG
                     EPAHKRVNPVLYRAEEVLAGWRRIAAPVLWVEGADTDVAKYWGDRYPRAEFEARLAQV
                     RQVERLVLADCGHMLHHDQPALLAARLREFLDRA"
BASE COUNT          553 a         1622 c         1621 g          553 t
ORIGIN      
        1 ctgccggcgt ctgccccagc ctcggcgccg ccgcccggac tcgacctgac cgtgactcgc
       61 gacgccatcc gcgcggccgc cgccgcgcag cccagcgtcg cccagatggc tcagctgcag
      121 acgcacgtgg ggccggcgac ctcggacgag cgcctgtcgc agggcgtggc cgcggccgtc
      181 aagccggact gcggtaccgc ggccgcgggt gcaggcctgt tcgcgctgcc ggtgctgatc
      241 tacgcggagg ccgccggcaa atgcaacaac tgacgcctcc gatcgatgcc agcgcgcggg
      301 cgccttcgcg gtacgcggcc gtgcagcccg cgccgcgagc ggacgtcgcc gccgacacgc
      361 ccgcccttcc cgccttcacg agcttcgccg acgtgcgcga catgttcacg ttcgtggcgt
      421 tcggcgcgcc ccgcttcgac tacgtgcggc gcctgcccgg cagcccgcgc gcgctcaccc
      481 tggagtccgc cttcgacgcg ctgcggcgcg gggtcgagcc cttcgagcgc cgcagcaagc
      541 gcggccgcca gcgggcgctg tatgcgcagg cgctggcgga gctggacgcg gcgctcgcgc
      601 actaccgcgc cggaaggcgc acgtacgggc gtctgacgct ggagctcgcg cgctgccgct
      661 tcgtcgaggc gggctcgacg caggcggccc cgagggcgcg cgccgacgcc gaagcgctgg
      721 cggcacggct agccgcgctg acctgagatc agcccgcccc ggcggcccgc tgcgcggcgc
      781 gatcctcctc gagccggcgc agcacgttgc gctgcagctt gccggtggtg gtcatgggca
      841 gcgcgtccac gaactcgatg tccttgggcg tctcgtaggg cgcgagccgg ctgcgcacca
      901 gctgctgcag cgcttcgacg agctccggcg tggccgcgtg cccggcggca aggatcacga
      961 aggccttgac gacggcgccg cggtcggggt cgggcttggg cacgacggcc acctgcgcga
     1021 ccgccggatg gcgcgcgagg cagtcctcga tctcggtcgg gccgatgcgg tagcccgagg
     1081 acttgaactg gtcgtcgctg cggccctggt accagaggta gccgtcctcg tccatcaccg
     1141 cgcggtcgcc ggtgcggcac caggactcga agggcttgcc ggtgaacttc gcgcgcgtgg
     1201 cgcggtcgtt gttccagtag ccgaggaaga agaccgggtc gcgatcgccg tgcacgtcgc
     1261 gcgcgtgcac cgcgacgtcg ccctcgacac cgggggcgca cggctcgccg tcgtcgtcga
     1321 tcacggccac gcggtggccc gggtagggcc ggcccatgct gcccggccgc ggcggccagc
     1381 cgggatgcgc gcggccgtcc cggtcgaccc agcggccgca gttgccgacg acgtagttca
     1441 tctcggtctg gccgaacatc tcgttgacga cgacgcccag cttgtcgcgg cagtaggcga
     1501 acacgccctc gccgaccgcc tcgccggcac tcatgatggc gcgcagcttc acgcccgggt
     1561 ggctcgtccg cggatcgggc gccacgcgca tcatcgcctt cagcgcggtc gggaacagga
     1621 aggtgtgcgt gacgccgtgg cgtgccatca ggcgcagcgc gaagtcggcg tcgaagcggc
     1681 cgccgaacgc gacgatggtg cggcccaggt acagcgtggg cagcagcgcg tcccacaggc
     1741 cgccggtcca cgtccagtcc gcggggctcc agaacgggcc gccgggctcg caccagttct
     1801 ggctcgcgac gaagccgctc aggttgccga tcagcgcgcg gtgggggatg cgcgcgccct
     1861 tggcgggccc ggtcgtgccg ctggtgtaga tcaggaacgc ggcgtcgtcg gcgcgggtgt
     1921 cggcggtctc gaactcggtg ctcgcgccgg cgagcgcgac actccaggcg aggtcgcagt
     1981 ccgcggcgac gaggccggtg ccgatcaccg tgcgcagcgc cgggcacgcc ggccgcagcg
     2041 cggcgatcgc ggcggtcgag ccgtcgtcca ccagcgcgac caccgcgccg ctgtcggcga
     2101 ggcgcgactg cagcgcctcc tgaccgaaca gcgtcgacag cggcaccgcg atcgcgccca
     2161 tctgcagcac cgccatcagc gcgatcgcca tctgcgggcg ctgcggcatc accacggcga
     2221 cgcggtcgcc cggctcgacg cccagcgccg cgagcgcgtt ggacagccgg cggctctcgg
     2281 cctgcagccg ggcgtagcgc caggccgtcg gcggcggcgc gtccgcggcg tcgccatggt
     2341 cgtggacgat ggccacggca tccggcgtct cgcgcgccca gcggtgcacg cacagctcgg
     2401 cgatgttcat gcgctccggc acgtgccagc gcaggccggc gtgcagcgcg gcgtggaagt
     2461 cctgggaagg ctcgggatcg gcgggcgtcg cggtcatggc cgcgagctta ggacgaatcg
     2521 cgccggccgt gcagcgggcc gcccaggatg tgaaccgccc tcaggcggcc gcggaggcca
     2581 tcgcggcgag cgcatcgccg gtcgcgcgca cgatgcgcca gtcgggcatc accgtcgcgc
     2641 ccatgcgcgc gtagaaggcc tgcgcgtcga cgttccagtc cagcaccgac cattcgaagc
     2701 gcccgcagcc gcgttcgacc gcgatcgcgg ccagccgctg cagcagccgc ctgccgagcc
     2761 cggtgccgcg cgcggcgggc tgcacgtaca ggtcctccag gtacaggccc ggccggcaca
     2821 ggaaggtcga gaagttgtgg aagaacagcg cgaacgcgac gacggccccg ccctgctccg
     2881 cgaccaggca ctcggcggcc ggccgctcgc cgaacaggtg cggctccagg cgctcgggcg
     2941 tcgcggtggc caggtgttcc agcttctcgt agacggcgag ctcgcggatc aggccgacga
     3001 tggcgggcag gtcggcgcgg gtggcgaagc ggatgtcgga ggtcggggtg gcggtcatcg
     3061 gccgattgtg gaccacctcg ccgtgctgtc gcgcaggcgt caagcccgcg tccgcgatct
     3121 cgcctacagt gcccgccatg cccgccatgc ccgccatgcc cgccatgtcc gccacgtccg
     3181 ccatgtccgc cccgaccgtc tacgccccgc ttcgcccggc ccgcaccgcc tcgcacgcca
     3241 tccgcacgat cgcgatgcag gtgcacgaat ggggcgacgc gtcgctcgcg acgccggagc
     3301 gtccgaccct ggtgctgatg cacggctgga tggacgtcgg cgcgtcgttc cagttcgtcg
     3361 tcgacgagct cgcgcggctc gagggcgagg cgcgccatgt gatcgcggcg gactggcgcg
     3421 gcttcggcgg cagcacgagc ggcgaggtgg acgcgttctg gttccaggac tacctcggcg
     3481 acctcgaggc cctgctcgac gcgttcgcgc ccgggcgggc cgtcgacctc gccggccaca
     3541 gcatgggcgg caacgtcgtg atgacctacg ccggagtgcg gccggcgcgc gtccggcggc
     3601 tcgtcaacct cgagggcttc ggcctgcccg actcgaagcc ggagcatgcg ccggcgcggc
     3661 tcgcgaaatg gctcgacgag ctgaagacgc cgcagtcgct gcggccctac gcctcgctcg
     3721 gggaggtcgc cgagcgcctt cgccgcaaca atccgcggct gagccccgaa cgggccgcgt
     3781 ggctggcgcc gcactggtcg cgacgtggcg ccgacggcct gtggcacgta ctcggggagc
     3841 cggcgcacaa gcgcgtcaac cccgtgctgt accgcgccga ggaggtgctc gccggctggc
     3901 gccgcatcgc cgcgccggtg ctgtgggtcg agggcgccga caccgacgtc gccaagtact
     3961 ggggcgaccg ctatccgcgc gccgagttcg aggcgcggct cgcgcaggtg cgccaggtcg
     4021 agcggctcgt cctcgccgac tgcggccaca tgctgcacca cgaccagccg gcgctgctgg
     4081 cggcgcggct gcgcgaattc ctcgaccgcg cctgaatccg gcgcgggaac gccggttgct
     4141 cgacggccgc ggacgggcgg cgccgccgca tcaccacgcg aggagttccc atggccgaag
     4201 atttccgcgc cgcgacgcgc gccgagctcg atgaaatggc cgccgacgcc ggcggtccct
     4261 cgccgcagga ggccgagaag gccgccgacg cgggcgccca ccgcgacccg ctgtcgggcg
     4321 aagtcggcgc gcatccgctg ggcgtggcg
//
LOCUS       DASVLN010000356         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_287667, whole genome shotgun sequence.
ACCESSION   DASVLN010000356 DASVLN010000000
VERSION     DASVLN010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287667"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1256)
                     /locus_tag="VF453_08730"
     CDS             complement(48..1256)
                     /locus_tag="VF453_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7687778.1"
                     /translation="MHGPSTTGLILTGGGARAAYQVGVLRAIARLRREAGAPAGNPYP
                     VLAGTSAGALNAAALATLADDPEAAVAKLVSVWEAMHVEQVYRTDSFGVVRTGARWLT
                     MMSMGWAIARMRRLRPRSMLDNRPLHDLLGAMIDLDRVDRLIADGHLQALAVSASSYT
                     SGAHVTFYQSASELKPWIRSNRLAVRESITMAHLLASAAIPFMFPAMPLIMSGQVEWF
                     GDGSMRQTAPISPAIHLGASRILVIGAGRMREPAGPRPNASDAYPTIAQIGGHALSSI
                     FLDALAVDAERLERINATLAMIPEELRSHAALRPVKLLLISPSERLDDIAARHLGALP
                     TSIRALLGAIGVTQGRSGSNGSALASYLLFESDYTRELMALGEADTLRRRAEVLEFFG
                     LPVAPGTTAG"
BASE COUNT          222 a          561 c          572 g          187 t
ORIGIN      
        1 gccggcagcc gtgacggacc gatcgcgggc cccaggcgcg gcacgcgtca gcccgcggtc
       61 gtccccggcg cgacgggcag cccgaagaat tcgagcacct cggcgcggcg gcgcagcgtg
      121 tcggcctcgc ccagggccat cagctcgcgc gtgtagtccg actcgaacag caggtagctc
      181 gcgagcgccg agccgttgct gcccgaacgc ccctgcgtca cgccgatcgc ccccagcagc
      241 gcgcggatcg acgtcggcag cgcgccgagg tggcgggcgg cgatgtcgtc caggcgctca
      301 gacggcgaga tgagcaagag cttgaccggg cgcagcgcgg cgtgcgagcg cagttcctcg
      361 gggatcatcg cgagggtcgc gttgatgcgc tccagccgtt ccgcgtcgac ggcgagcgcg
      421 tcgaggaaga tgctcgagag cgcatggccg ccgatctgcg cgatcgtcgg ataggcgtcg
      481 ctcgcgttcg ggcgcggacc ggcgggctcg cgcatgcggc cggcgccgat cacgaggatg
      541 cggctcgcgc ccaggtggat cgcgggcgag atcggcgcgg tctggcgcat cgagccgtcc
      601 ccgaaccact cgacctggcc cgacatgatc agcggcatcg cggggaacat gaacgggatc
      661 gccgccgaag cgagcaggtg cgccatcgtg atcgattcgc gcaccgcgag gcgattggag
      721 cggatccagg gcttcagctc ggaggccgac tggtagaacg tgacgtgcgc gcccgaggta
      781 tagctcgacg cgctcacggc cagcgcctgc aggtggccgt cggcgatcag gcggtcgacc
      841 cggtcgaggt ctatcatcgc gccgagcagg tcgtgcagcg gccggttgtc cagcatcgag
      901 cgcggtcgca gcctgcgcat gcgcgcgatc gcccagccca tcgacatcat cgtcagccag
      961 cgcgccccgg tgcgcacgac gccgaaggag tcggtacggt agacctgctc gacatgcatg
     1021 gcctcccaca ccgagaccag cttcgcgacg gcggcttcgg ggtcgtccgc cagcgtcgcg
     1081 agcgccgccg cgttcagcgc gccggccgac gtgcctgcca gcaccgggta ggggttgccc
     1141 gccggagccc ccgcctcgcg ccgcaggcgc gcgatcgccc gcaatacccc cacctggtag
     1201 gccgcgcgcg cgccgccgcc cgtgaggatc agacccgtcg tcgatggacc gtgcatggcg
     1261 cgcagtatgc ggcaacgcga cggtgggacg ggccggtccc ccgcgagccg cggcgcggcc
     1321 tcgtgcccga tcacacgctt gcgcgagtcg agcgcatcgc ggacgcgctg ccggcgcgag
     1381 aggtcacgcg cacgcgtcct gcggaactcg cacgggggat gcccgcaggc gcaggatcag
     1441 gcccgcgccg ggacgcgcgc gagcaccgtg cccgactcag cgatcctgga ggcgatgccc
     1501 gccgccgtcc cgacccgcgc cgcgatcgcc gtgatcgggc gc
//
LOCUS       DASVLN010000357         7309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_180120, whole genome shotgun sequence.
ACCESSION   DASVLN010000357 DASVLN010000000
VERSION     DASVLN010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180120"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VF453_08735"
     CDS             <1..617
                     /locus_tag="VF453_08735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX7687779.1"
                     /translation="AHRLATIRMDSAPRWIALFLLVEFAYYWHHRASHRVRWFWATHS
                     VHHSSSELMLASAIRLGWTSRLGGALLFYAPLAWLGFPVPAVLGLAAAGLFYQFWIHA
                     DWLPRLGPLEWVLNTPSHHRVHHASNPEYLDCNYGSTLIVFDRLFGTFVDERADIAIR
                     YGLVHPLASRNPLRIAFHEWLALARDLRAARGTRARLRLVFGPP"
     gene            713..1648
                     /gene="lpxC"
                     /locus_tag="VF453_08740"
     CDS             713..1648
                     /gene="lpxC"
                     /locus_tag="VF453_08740"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX7687780.1"
                     /translation="MLQQRTLNSITRAVGVGLHSGQKVELTLRPAAPDTGIVFRRVDL
                     PQPVDIPVDAMAVSDTRMASTISPGGDPGGPKVQTIEHLMSACAGLGIDNLVVDITAD
                     EVPILDGSSASFVFLLQSAGIKLQDAPKRFVRVRKPVEIRRGEGASLSWCRLEPYHGF
                     KLTFEIEFNHPAVDATGQQVTFDMGSGQYKRDIARARTFGFTKDVEMMRSRGLTLGAS
                     MENAIVVDDSRVLNADGLRYDDEFVKHKILDAIGDLFVVGHPLLASYTAYKSGHAMNN
                     QLLRALLADPAAYEIVTFEDERLAPAGFAELAPAW"
     gene            complement(1665..1940)
                     /locus_tag="VF453_08745"
     CDS             complement(1665..1940)
                     /locus_tag="VF453_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687781.1"
                     /translation="MRSISERDLAVVIPLLALKIRELEQELREIEARPGARTDAEVED
                     RQQIQDMLSQYDGILETLRDEYEQGLTEGINLPSYDDLTRRILLQRD"
     gene            complement(1969..2484)
                     /locus_tag="VF453_08750"
     CDS             complement(1969..2484)
                     /locus_tag="VF453_08750"
                     /inference="COORDINATES: protein motif:HMM:NF037873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687782.1"
                     /translation="MADVPVDLFRSCKSSRWKGNTPTKKNDNFTSAEGQGSLFPDYVG
                     FTRRDGSVRAPDVTTFTDREGTTWVRGVEDRDDRDRPFVSWKEGVSVSTEPGGFGYEG
                     WFYFLVPAGTPVPDSLDVKPTPTRSDPGHHSIRCRNLMRRDAYEGALGNLTRAALAKA
                     VEMGKQSFRHS"
     gene            complement(2801..3769)
                     /locus_tag="VF453_08755"
     CDS             complement(2801..3769)
                     /locus_tag="VF453_08755"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7687783.1"
                     /translation="MKRTLDGVSASETRWLAQAFARGHRLRKSYGDPGSFLESTISEG
                     SAEEQPATSTPWTGGYRCGLSMRRPSLRAIVLAVSCSGFMIACSAASPTNQAAMSSNR
                     AVFFTSGAGAALEEAIRANDPSAITRALAGGASVNAVSKQGVSPLMIAVDAQKLSAVR
                     ALLQAGANPNLKAADGRSAVSLAVENYVAQPDGRAIMLAVMNGGGDPNTLRPDGDPVI
                     RRFLNDHALDDVRLFKQFGANLDIIGRGNEPLITSVALAQDWDGVWCLIELGARYDYE
                     NGKSTQPISDALSPRFPAPDSPMYPYKVKVWELMRDKGLPVKPLRH"
     gene            3844..4563
                     /locus_tag="VF453_08760"
     CDS             3844..4563
                     /locus_tag="VF453_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687784.1"
                     /translation="MGACDRGTAGLDDAISAPRMTTDAALPRLVAGLGDTLADVRQHS
                     TFPFRTDSLNDRYVVAVDKPIRFVFAGPRPFELEPARFASLECTAAIVTAIQASPHLD
                     YLTQADALALLAQLRERVSQAGWEVDRDERPVPTGAELALSLRDPALPDDTWWFAAIY
                     RSGDARLFVKLRRAHRAGRNGPDALFLVTLQWRDDVLLDRAIALARHLRQQDGLPPDV
                     DRPIVLDAYVPRVRALMPRAS"
     gene            complement(6097..7125)
                     /locus_tag="VF453_08765"
     CDS             complement(6097..7125)
                     /locus_tag="VF453_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687785.1"
                     /translation="MSQVSRLYDRQGGSPHVGDHATGVLASTVPHSDLLPTYSPDAQL
                     GYAYARFANTDQQFIGVSAGGVLARSNVATGGAVWEWWWAGLQFVNDYDYGRQMQSAA
                     YFNGYSLSEAGDEYGGSTINVDVRHPSPIALATTSGNTQSTLAVPLEYFPERFGGGPA
                     NPVIYPDATIGKNLTLDWVGPDGVDRNWPVAVYETHVNVPAPVVEAIVEAPTAYIDST
                     FHYYYKMTNFTTGATTPIATVPLQATNVSAGGVQAIILSSAASTSPNAAAMGVLISDP
                     NAGLVIYDNASGASPGQYGSNFAKWEVHYDSALAPGWKFKTWIVTDTYANVVADLVQL
                     AKWGLSSR"
BASE COUNT         1146 a         2430 c         2441 g         1292 t
ORIGIN      
        1 acgcgcaccg gctcgcgacc atccgcatgg actcggcgcc caggtggatc gcgctgttcc
       61 tgctcgtgga gttcgcctac tactggcacc accgcgcctc gcaccgcgtg cgctggttct
      121 gggccacgca ctcggtgcac cattcgtcga gcgagctgat gctggcctcc gcgatccgcc
      181 tcggatggac cagccgcctc ggcggcgcgc tgctgttcta cgcgccgctc gcgtggctcg
      241 gcttccccgt gccggcggtg ctcgggctcg ccgcggcggg gctgttctac cagttctgga
      301 tccacgcgga ctggctgccg cggctcgggc cgctcgagtg ggtgctcaac acgccgtcgc
      361 accaccgcgt ccaccacgcg agcaacccgg agtacctgga ctgcaactac ggcagcacgc
      421 tgatcgtctt cgaccgcctg ttcggcacct tcgtcgacga gcgtgccgac atcgcgatcc
      481 gctacggcct cgtgcatccg ctcgcgagcc gcaacccgct gcgcatcgcg ttccacgaat
      541 ggctcgccct cgcgcgcgac ctgcgcgccg cgcgcgggac gcgggcgcgg ctgcggctcg
      601 tgttcgggcc gccgtgacgc gcgcgcggga cggccgcgcg atggccctgg cctatcgccg
      661 ccattgacgc gacagcaacg tccccgcaag gcgcgccgcc taaaatcgcg gcatgctcca
      721 gcaacgcacg ctcaactcga tcacgcgcgc cgtcggcgtg ggcctccaca gcggacagaa
      781 ggtcgagctc acgctgcgcc cggccgcgcc ggacacgggc atcgtcttcc gccgcgtcga
      841 cctgccgcag ccggtcgaca tccccgtcga cgcgatggcc gtctccgaca cccgcatggc
      901 ctcgacgatc tcgcccggcg gcgatcccgg cggccccaag gtgcagacga tcgagcacct
      961 gatgtcggcc tgcgcgggcc tgggcatcga caacctcgtg gtcgacatca cggccgacga
     1021 ggtgcccatc ctcgacggct cgtcggcgag cttcgtgttc ctgctgcaga gcgccggcat
     1081 caagctgcag gacgcgccca agcgcttcgt gcgcgtgcgc aagccggtcg agatccgccg
     1141 cggcgagggc gcctcgctca gctggtgccg gctcgagccg taccacggct tcaagctcac
     1201 cttcgagatc gagttcaacc atcccgcggt cgacgccacc ggccagcagg tgaccttcga
     1261 catgggcagc ggccagtaca agcgcgacat cgcccgcgcc cgcaccttcg gcttcacgaa
     1321 ggacgtcgag atgatgcgct cgcgcggcct gacgctgggc gcgagcatgg agaacgcgat
     1381 cgtcgtcgac gactcgcgcg tgctcaacgc cgacggcctt cgctacgacg acgagttcgt
     1441 gaagcacaag atcctcgacg ccatcggcga cctgttcgtc gtcggccacc cgctgctggc
     1501 gagctacacc gcctacaaga gcgggcacgc gatgaacaac cagctgctgc gtgcgctgct
     1561 ggccgatccg gcggcctacg agatcgtgac cttcgaggac gagcggctgg cgccggccgg
     1621 gttcgcggag ctggcgccgg cctggtgagg cggcgaaagg cagttcaatc gcgctggaga
     1681 aggatgcggc gcgtcaggtc gtcgtagctc ggcaggttga tgccttcggt caggccttgc
     1741 tcgtattcgt cgcgcagggt ttcgaggatg ccgtcgtact gcgacagcat gtcctggatc
     1801 tgttgcctgt cctcgacttc ggcgtccgtg cgggcgcccg gccgtgcctc gatctcgcgc
     1861 aattcctgct cgagctcgcg gatcttgagg gccagcagtg gaatcaccac ggccaggtct
     1921 cgttcgctga tggatcgcat gcgggttcct cgcggggtgg ttgtcgtctc aggaatggcg
     1981 gaaggactgc ttgcccattt cgaccgcctt ggccagcgcc gcgcgtgtca ggttgccgag
     2041 cgcaccctca taagcgtcgc ggcgcatgag atttcgacag cggatcgaat gatgccccgg
     2101 atcgctgcgc gtgggggtcg gcttgacgtc cagtgaatcg ggaacgggcg tgcccgcagg
     2161 caccaagaaa tagaaccacc cttcatagcc gaagccgccg ggttcggtgc tgacggagac
     2221 gccctccttc cagctgacga agggccggtc tcggtcgtcc cggtcttcga cgccgcgcac
     2281 ccaggtcgtg ccctcgcggt cggtaaaggt cgtgacgtcg ggcgcccgca cggagccgtc
     2341 ccgccgcgtg aagccgacgt agtctggaaa gaggctgccc tggccctccg cgctggtgaa
     2401 gttgtcgttc ttcttggtcg gggtgttccc cttccagcgc gaggacttgc acgatcggaa
     2461 caggtcgaca ggaacgtccg ccatgggcga tttctccgaa aagtgagtgc ccggcatggt
     2521 atccgccgca acgcgcaaag gcacccccat tgcgagggtg gcgagacgac ggtaagagcg
     2581 agcctcgagg tcaagtctcg cctggcaatc aagccgccgg tctgccggcg ctctgacgct
     2641 tgagacgtta acgagcttgt tgcaagctac ttgccacgcg caagacctga ccccggcttc
     2701 ccgaccccgg cttcccgccc tgcctatgca attcgcggac cagatgtgcg cctcggcccc
     2761 tcggggtctc ggcagcaaga gcgctgccgg cgtttcggtc tcaatgccgc aacggcttga
     2821 ccggcagccc cttgtcccgc atcaactccc agaccttgac cttgtacgga tacatgggcg
     2881 agtccggcgc gggaaaccgc gggctcagtg catcgctgat cggctgggtc gatttcccgt
     2941 tctcgtagtc gtagcgcgcc ccgagctcga tcaggcacca gacgccgtcc cagtcctgcg
     3001 cgagcgccac gctggtgatc agcggctcgt tgccgcggcc gatgatgtcg agattggcgc
     3061 cgaactgctt gaacaggcgg acgtcgtcca gggcgtggtc gttgaggaac cggcggatga
     3121 ccggatcgcc gtccggccgc aacgtgttcg ggtcgccacc gccgttcatg acggcgagca
     3181 tgatggcgcg gccatcgggt tgggcgacgt agttttcgac cgcgaggctg accgcgctgc
     3241 gcccgtccgc ggccttcagg ttcgggttgg cgccggcctg cagcaaggcg cggacggccg
     3301 acagcttctg cgcatcgacg gcgatcatca gcggggagac gccctgcttg ctgacggcgt
     3361 tgaccgacgc gccgccggcg agcgcgcgcg tgatggcgga gggatcgttg gcacggatcg
     3421 cctcctcgag cgccgcgccg gcgccggagg tgaagaagac ggcacggttg gaggacatcg
     3481 cggcttggtt cgtgggggac gcggctgaac aggcgatcat gaagccgctg cacgagacag
     3541 ccaggacgat cgcgcggagg gacgggcgcc tcatggacaa tccgcaacgg tacccgcctg
     3601 tccagggggt gctcgtggcg ggctgctctt ccgccgatcc ttcggagatc gtcgactcca
     3661 ggaagctccc tgggtcgccg tacgacttcc ggagccgatg gcctctcgca aaggcttgtg
     3721 ccaaccaacg ggtctcgctg gccgatacgc catcgagcgt gcgcttcatc ctgcgttcat
     3781 cgaatgccat cgctgatgcc tctcccgggc cgcagggcct ttctcctggc cgtggcgtcc
     3841 agcatgggcg cgtgcgacag gggcacggcc gggctcgacg atgccatatc ggcgcctcgg
     3901 atgaccaccg acgccgctct tcctcgcctc gtcgccggcc tgggcgacac cctggcggac
     3961 gtgcgccagc actcgacatt cccgttccgc acggactcgt tgaacgacag atacgtggtc
     4021 gctgtcgaca agccgattcg gttcgtcttc gcgggaccgc gtcctttcga gctcgagccc
     4081 gcccggttcg cgtcgctcga gtgcaccgcg gcgatcgtca cggccatcca ggcttcccct
     4141 cacctcgact acctgacgca ggcggacgcc ctggcgctgc tggcgcagct gcgcgaacgc
     4201 gtctcgcaag ccggctggga ggtcgatcgc gacgagcggc cggtgcccac cggcgccgag
     4261 ctggcgctga gcctgcgtga cccggcgctg ccggacgaca cctggtggtt cgcggccatc
     4321 taccgcagcg gcgatgcgcg cctgttcgtc aagctgcggc gggcgcaccg tgccggacgc
     4381 aatggcccgg acgccctgtt cctcgtgacg ctgcaatggc gcgacgacgt cctgctcgat
     4441 cgcgccatcg cactggccag gcaccttcgc caacaggacg gcctgccgcc ggacgtggat
     4501 cgaccgatcg tgctggacgc gtacgttcca cgggtgcgtg ccttgatgcc cagggcttcc
     4561 tgagaaggtt gttggaatct ccgccaagcg ttgtcgcgca ggtcttgtcc gccgtgaggt
     4621 tccactttga gtcccgtcgg cgcggcgcgc ctgtgggggt ggtgggtcag cgggtcggcg
     4681 gcgacaggcc ggggaggtgt cttggctctc cggctctcgt gcatctggaa atcggcctgg
     4741 acggccgatt tcccgcgtgg gcgttcggtg aagattcgcg cccggcaacg cacgcctggc
     4801 tgcgtcctgg aagtactcct tcggttccac catgcccgcg acgtgccgac ccgcctgcga
     4861 tccaagacac ctgcccggcc tgtcgccgcg gcttcttctg tgggaagagt gggcgtgcct
     4921 cggcccatcg agggcctcgg cagcaagagc gcgccggcgc ctcggcggtt ccggcggcgt
     4981 cttcccggcg ctacgaccgt agtcttgacc ggcagatcca ggccgtcggc gtcctcgtgt
     5041 acggcatcga gtcgcgagat tcactcgccg cctcgtcagt tcatcgccgg aatgaaccag
     5101 ttcgacgttt cattgaatca attccactcc gagtagaact gactccccgt tttggtgcgt
     5161 tcactatgtc cgccattgaa ttgattcgtc gcggagagga actgattatg tccggagtcc
     5221 agtgggttca atcggcggat gaatcaattc gacggcgcgg tgaattaatt catcgaaaag
     5281 ggaaactcgc tggatcgcga gggaagtccg cgcaccggcg cggtgaattc ggttctccat
     5341 gcgatgaatg aattcatctg ggcggggaac tgggaggtgg cgcgggtgag gtctcgaggc
     5401 tgagcggtgt accggatcat ggcggtgagc ggccggctgc ctcggcgtgg ttgccatgcc
     5461 ggttggcccg tgtagcggcc tcgcagcgcg ctcgccaagc cgcgcggggc gagtccgccc
     5521 atgatacggg ggcgggctgc cgatgcctcg caagttggcg cggcaagccg agaagggttc
     5581 gacattgcga ccgcgcggaa tgcggcagtg gatgaccgca ctgcggccgc tgtggccttg
     5641 ccgtcggcac ctgcctttgg ccataggcct cgctccgcgg tcgacacgcg atggctgctt
     5701 tggcggtcgc agcagtcgct tgttccatgg agggaggtgc cggcttccgc tgcattgccg
     5761 tccctggcgg ggtgacctcg acgggttggc cggttgcctc cgccgaatgc aaatttttgg
     5821 ccggttcgtg ccggcgcctc gctggcatgt ggccgcgtca ccgaccggtc tggggcggac
     5881 agctgacgct caggagcggc gccgccaggg ctccgggtcg gttggcgccc gaatgaccgc
     5941 tgttccggtc tgcaactcac gctctcgacc cgttgctgtc gtcccatctc tcaaactccg
     6001 cgcccctgag cggtcatcgg aagccacaaa cgagatgccc gcacacgcct acttcacgcg
     6061 tcttccggga tttgcgacag tttgacctcg gaacgatcag cgcgacgaca atccccattt
     6121 ggcaagctgc accagatcgg cgacgacgtt cgcataagtg tcggtcacga tccaggtttt
     6181 gaacttccaa cccggtgcca aggccgagtc gtagtggacc tcccatttgg cgaagttgct
     6241 gccgtactgg ccgggggaag cgccggatgc gttgtcgtag atcacaagtc cggcgttcgg
     6301 atcgctgatt aggacgccca tggccgcagc gttgggactg gtcgacgccg cagatgacaa
     6361 gatgatcgcc tgaacgccgc cggcagagac attggtggct tggagtggca cggtcgcgat
     6421 gggtgttgtc gcgcctgtcg tgaagttggt catcttgtag taatagtgaa acgtagagtc
     6481 gatgtacgcg gtcggcgcct cgacgatggc ctcaaccacc ggagccggaa cattgacatg
     6541 cgtctcgtac acggcgacgg gccagttgcg atcgacacca tctggaccga cccagtcaag
     6601 tgtcaggttc ttcccgatgg tcgcatcagg atagatcacg ggattggccg ggccgccccc
     6661 gaaccgctca gggaagtatt cgagcggcac tgcgagggtc gattgcgtgt tgccagacgt
     6721 cgtggcaagc gctatagggg acggatgccg gacgtcgacg ttgatggtcg aaccgccgta
     6781 ttcgtcgccc gcttcgctca gcgagtagcc gttaaagtag gctgcagact gcatctggcg
     6841 accatagtcg tagtcattca caaactgcaa gcccgcccac caccattccc agaccgcgcc
     6901 gcccgtcgcg acgttcgagc gcgccaggac gccaccggcg gaaacgccga tgaattgctg
     6961 atcggtgttc gcgaatcgtg cataggcgta gccgagttgc gcatccggcg aatacgtagg
     7021 cagcaaatct gaatgcggta cggtcgatgc gaggacgccc gtcgcgtgat caccgacatg
     7081 cggcgatccg ccttgacggt catagagtcg cgagacctgg gacaaaccat gcgccgcaga
     7141 gctaccgctc cacacgtagc ccagtgtccc tagggacagt ctgcaaaact ccgggcggcc
     7201 ccgcgtagcg tgaagagcgg cgccggacga aaatcgggcc catgaaacag ctcggacttg
     7261 gcctgaacct gtcgacgaag aagacgcgca agcgcgagtt cctcgagga
//
LOCUS       DASVLN010000358         7996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_394093, whole genome shotgun sequence.
ACCESSION   DASVLN010000358 DASVLN010000000
VERSION     DASVLN010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394093"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VF453_08770"
     CDS             complement(<1..584)
                     /locus_tag="VF453_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX7687786.1"
                     /translation="MYLGLDLGTSALKALLLDDEGRIVAQRSAALEVSRPRPLWSEQA
                     PADWWRALEGAVGALKASHARELGAVRAIGLSGQMHGAVLLDAAGAVLRPAILWNDGR
                     SAPQCDALAARLPALPEIAGNLAMPGFTAPKLMWVAEHEPAIFARVATVLLPKDWLRF
                     ELTGERFSDCSDASGTLWLDVARRDWSDELLAASG"
     gene            complement(648..1574)
                     /locus_tag="VF453_08775"
     CDS             complement(648..1574)
                     /locus_tag="VF453_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7687787.1"
                     /translation="MHSTPRVRAPEIEHDHLRDPVLGYDPPEGCGFLRCLAHGIPSPL
                     VRWHHHDEYELHLIVATSGKVFVGDYIGHFQPGHLVLTGPRLPHNWISSDAPPAGVAL
                     RDRVIQFAHEPIEQACELFPEFKEILPMLEQARYGIEFFGVSDVALAHFERVAGSRGL
                     ARIAGFCEFMSMLAHCKDFRLLSSVQLQCSEDDESMAQVNAVVSYISEHCNEPLSMAD
                     VCARFRMPESRFSRFFRRATGNTFTDFVNRMRISKACQLLMETDRYVTTICYEVGFNN
                     VANFNRRFLEIKGMTPTDFRRQAASRFGGSAH"
     gene            complement(1614..3032)
                     /gene="dalD"
                     /locus_tag="VF453_08780"
     CDS             complement(1614..3032)
                     /gene="dalD"
                     /locus_tag="VF453_08780"
                     /EC_number="1.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047813 - D-arabinitol 4-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0051161 - arabitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinitol 4-dehydrogenase"
                     /protein_id="HEX7687788.1"
                     /translation="MASNLTAPAGRLRLLHLGLGSFHRAHQAVYMHRLLQSGDARWQC
                     AGANLRPDMADTIAALERQGGAYTLETVSPQGEHVLEKITSITEVVPFEPTLARVLAL
                     GADPDTRIVSFTVTEAGYALGHDGRIDLAHPEVRADLAGASLATIYGVAAAILRARRA
                     AGAGPLTFLNCDNLRHNGDHFRANLETFLHAAGDASLLEWLRANTAFPNAMVDRITPR
                     PTPEAMARIRAQAGWDDQAPLMAESFIQWVVEERFAAGRPDWEKVGVEMTRDVQPYEE
                     AKIRILNASHSAIAWAGTLVGLNFIHEGTRMPAIRAIAHAYVTDDVIPCLTQPGRESP
                     VDLARYRDVVLDRFANDAIADTNQRVAADGFAKIPGFIAPTIRERLGRGESIASVARL
                     PALFLAFLRRWHAGRLPFAYQDQAMVPERAHAICEAADPLAAFVAEASLWGELAGSAA
                     LRDAVDAATLDLRARLGDNAMN"
     gene            complement(3090..4109)
                     /gene="ugpC"
                     /locus_tag="VF453_08785"
     CDS             complement(3090..4109)
                     /gene="ugpC"
                     /locus_tag="VF453_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX7687789.1"
                     /translation="MAFLELKHVVKQFGDVRTIKGVDLSIDRGQFTVFVGPSGCGKST
                     LLRLIAGLETLSEGSIHLDGREITHLPSAQRDLAMVFQSYALYPHMTVAENMSFSLRL
                     AKADPAVIKKKVDRAAEILNLGPYLKRLPREMSGGQRQRVAIGRAIVREPKVFLFDEP
                     LSNLDAALRGQTRVEIAKLHRELGATTIYVTHDQVEAMTLADRVVVLRDGNIEQVGSP
                     LELYDRPANRFVAQFIGTPQMNIVERAELPAAAGHAGAAFAGIRPEALHLAPQGQGQI
                     AARVELVEALGAETLVYLHTPKGSQIVLRQNARTTLAAGDQVGVDVELDHLHWFDGNG
                     RVLAA"
     gene            complement(4122..4964)
                     /locus_tag="VF453_08790"
     CDS             complement(4122..4964)
                     /locus_tag="VF453_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7687790.1"
                     /translation="MNANTVPGTHGLLFLALRTVAAWLVALLLFFPILFMLLTSFKTE
                     LQAIAVPSLWIFHPTLENFTHVQERSDYLLYARNSLVTSVVSTLLGLAIAAPAAYSMA
                     FFPTKRTKDILMWMLSTKMMPAVGALVPVYVMAQSSRLLDTCTGLIIVFTLSNLPLMV
                     WMLYSHFKDIPPEILEAARMDGASLAAEFRHVVLPLAMGGLASTGLLCLVLSWNEAFW
                     SLNLTAANAGTLAALIASYSSPEGLFWAKLSAASVLAIAPIVVFGWFSQKQLVQGLTF
                     GAVK"
     gene            complement(5047..5904)
                     /locus_tag="VF453_08795"
     CDS             complement(5047..5904)
                     /locus_tag="VF453_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7687791.1"
                     /translation="MRSLARLLLAPAVGSLFLWMIVPLAMTLYFSVIRYNLMEPGEHA
                     FVGLENFRFFITDPSFWPAILNTLLLLGSVIAITVVLGVLIALLVNEPFPGRGIVRVL
                     LISPFFVMPTVNALLWKHMMMNPIYGVLAQVWRALGLTPVDWLGDWPLLSVIIMVAWQ
                     WLPFAVLIFITSLQGMNREQLEAARMDGANWFQQFRFLVLPHLARSIAVVVMIELIFL
                     LSVFAEISTTTGGGPGDASTNIAYLIFKQALLGFDVGVASAGALFAVVLANIAAAFLI
                     RLVGKNMER"
     gene            complement(5992..7314)
                     /locus_tag="VF453_08800"
     CDS             complement(5992..7314)
                     /locus_tag="VF453_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7687792.1"
                     /translation="MTRNASPARAVALAASLAIAASFPAHATDITIATVNNGHMIEMQ
                     KLTHFFEQANPDIHVKWVTLEEGVLRQRVTTDIATKGGQFDVMTIGMYETPIWGKKGW
                     LQEIKPDASYDVDDLLPAIRNGLSADGKLYAAPFYGESSMLMYRKDLAAKAGVTFSDR
                     PTWDQVRAAADKMTDPKAGVYGICLRGKPGWGDNMAFLTTLVNSFGGQWFDMSWKPQL
                     DSKPWHDAINFYVDMMKKDGPPGSSANSFNEILALYNEGKCAMWDDATIAASFITDPK
                     QSKVADKVAFTQAPQQVTAKGANWLWAWALAVPASSKNADAAQKFVNWATSKAYIQLV
                     AKEEGWAHVPTGTRKSTYANPEFLAVAKFATAEAKAIDSANPLDPTLPKSPYTGVQFA
                     TIPEFQSIGVTVGQQMSAALAGKESVDAALKASQVAAERELKKSGRLK"
     gene            7730..>7996
                     /locus_tag="VF453_08805"
     CDS             7730..>7996
                     /locus_tag="VF453_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX7687793.1"
                     /translation="MTYPMPYRTALLAAAAASALLASAPARAVDWTGYMRGGPAATTE
                     SGHSRQCYGIGELKYRLGNECDFYGEFQLAQAMKVDGVDVNAVLM"
BASE COUNT         1258 a         2662 c         2877 g         1199 t
ORIGIN      
        1 ccactcgcgg caagcagttc gtccgaccag tcgcgcctcg ccacatccag ccacagcgtg
       61 cccgacgcgt ccgagcagtc gctgaagcgc tcgcccgtca gctcgaagcg cagccaatcc
      121 ttgggcagca gcaccgtcgc gacgcgggcg aagatcgccg gctcgtgctc ggccacccac
      181 atcagcttgg gcgcggtgaa gccgggcatc gcgaggttgc ccgcgatctc gggcaacgcg
      241 ggcaggcgtg ccgctagcgc gtcgcactgc ggcgcgctgc ggccgtcgtt ccaaaggatc
      301 gccggccgca ggacggcgcc cgccgcgtcg agcagcacgg cgccgtgcat ctgtcccgac
      361 agcccgatcg cccgcaccgc gccgagctcg cgcgcatgcg aggccttgag cgcaccgacc
      421 gctccctcga gcgcccgcca ccagtcggcc ggcgcctgct cgctccacag cgggcgcggg
      481 cgcgagacct ccagcgcggc gctgcgctgg gcgacgatgc ggccttcgtc gtccagcagg
      541 agcgccttca gggcggacgt tccgaggtcg aggccgaggt acatggcggg gtatccgtga
      601 agaatgcgag cgcacccgac gtcgcgcgcg cccacgggcg cgacgcgtca gtgcgcagag
      661 ccgccgaagc gcgaggccgc ctggcgccgg aagtcggtgg gcgtcatgcc cttgatctcc
      721 aggaagcggc ggttgaagtt ggcgacgttg ttgaagccga cctcgtagca gatcgtcgtc
      781 acgtagcggt cggtctccat cagcagctgg caggccttgc tgatgcgcat gcggttgacg
      841 aagtcggtga acgtgttgcc ggtcgcgcgg cggaagaagc gcgagaagcg gctctcgggc
      901 atgcgaaagc gcgcgcagac gtccgccatg gacagcggct cgttgcagtg ctcgctgatg
      961 tagctgacca ccgcgttgac ctgcgccatc gactcgtcgt cctccgagca ctgcagctgc
     1021 acgctggaca gcagccggaa gtccttgcag tgcgcgagca tggacatgaa ctcgcagaag
     1081 cccgcgatgc gcgcgagccc gcgcgagccc gccacgcgct cgaaatgcgc cagcgcgacg
     1141 tccgagacgc cgaagaactc gatgccgtag cgcgcctgtt cgagcatcgg caggatctcc
     1201 ttgaactcgg ggaagagctc gcaggcctgc tcgatgggct cgtgcgcgaa ctggatcacg
     1261 cgatcccgca gcgccacgcc ggccggcggt gcgtcgctgg agatccagtt gtgcggcagc
     1321 cgcgggccgg tcagcaccag gtgtccgggc tggaagtggc cgatgtagtc gcccacgaag
     1381 accttgccgc tggtcgcgac gatcaggtgc agctcgtact cgtcgtggtg gtgccagcgc
     1441 accagcggcg aggggatgcc gtgcgcgagg cagcgcagga agccgcagcc ctcgggcggg
     1501 tcgtagccga ggacggggtc gcgcaggtgg tcgtgctcga tctccggcgc gcggacgcgg
     1561 ggcgtggagt gcatggcggc ctcgtgggtg cgtgtgcggg cgacggcgcc ggctcagttc
     1621 atcgcattgt cgccaagacg ggcgcgcaga tcgagcgtcg cggcgtccac tgcgtcacgc
     1681 aacgcggcgc tgccggcgag ctcgccccac agcgacgcct cggcgacgaa ggccgcgagc
     1741 ggatccgccg cctcgcagat cgcatgcgcg cgctcgggca ccatcgcctg gtcctggtag
     1801 gcgaacggca gccggccggc atgccagcgc cgcaggaagg cgaggaacag cgcgggcagg
     1861 cgcgcgaccg acgcgatcga ttcgccgcgc ccgagccgct cgcggatcgt gggcgcgatg
     1921 aagcccggga tcttggcgaa gccatcggcg gccacgcgct ggttggtgtc ggcgatggcg
     1981 tcgttggcga agcggtcgag cacgacgtcg cggtagcgcg cgaggtcgac cgggctctcg
     2041 cgccccggct gcgtgaggca ggggatcacg tcgtcggtca cgtaggcgtg cgcgatcgcg
     2101 cggatcgccg gcatgcgcgt gccctcgtgg atgaagttca ggccgaccag ggtgccggcc
     2161 catgcgatcg cgctgtggct ggcgttgagg atgcggatct tggcttcctc gtagggctgg
     2221 acgtcgcgcg tcatctcgac gccgaccttc tcccagtccg gccggccggc ggcgaagcgc
     2281 tcctcgacca cccactggat gaaggactcg gccatcagcg gcgcctggtc gtcccagccg
     2341 gcctgcgcgc ggatgcgggc catcgcctcg ggcgtcgggc gcggcgtgat gcggtcgacc
     2401 atcgcgttgg ggaaggccgt gttggcacgc agccactcga gcagcgaggc gtcgccggcc
     2461 gcgtgcagga aggtctcgag gttggcgcgg aagtggtcgc cgttgtggcg caggttgtcg
     2521 cagttcagga aggtcagcgg gccggcgcca gccgcgcgac gcgcgcgcag gatcgcggcc
     2581 gcgacgccgt agatcgtcgc gagcgaggcg cccgcgaggt cggcccgcac ttcgggatgc
     2641 gcgaggtcga tgcgcccgtc gtgcccgagc gcgtagcccg cctcggtcac cgtgaacgag
     2701 acgatgcgcg tgtccggatc ggcgcccagc gcgagcacgc gcgccagcgt cggctcgaac
     2761 ggcaccacct cggtgatgga cgtgatcttc tccagcacat gctcgccctg cggcgacacc
     2821 gtctccagcg tgtaggcgcc gccctggcgc tcgagcgcgg cgatggtgtc ggccatgtcg
     2881 gggcggaggt tggcgccggc gcactgccag cgcgcgtcgc ccgactgcag caggcgatgc
     2941 atgtagacgg cctggtgggc gcggtggaac gagcccaggc cgagatggag caggcgcagg
     3001 cggccggccg gcgcggtgag gttggaagcc atgttctgta ctggaaggga gatcggtggg
     3061 cgcgcgccgg cggcagcgtc gggatagcgt caggcagcca ggacgcggcc gttgccgtcg
     3121 aaccagtgca ggtggtcgag ctcgacgtcg acgcccacct ggtcgcctgc cgcgagcgtg
     3181 gtgcgcgcgt tctgccgcag cacgatctgc gaacccttgg gggtatgcag gtagaccagc
     3241 gtctcggcgc ccagcgcctc gaccagctcg acgcgcgccg cgatctggcc ctggccctgc
     3301 ggcgcgaggt gcagcgcctc ggggcggatg ccggcgaacg cggcgccggc gtggccggcc
     3361 gcggccggca gctcggcgcg ctcgacgatg ttcatctgcg gcgtgccgat gaactgcgcg
     3421 acgaagcggt tcgccggccg gtcgtacagc tccagcggcg agccgacctg ctcgatgttg
     3481 ccgtcgcgca gcaccacgac gcggtcggcc agcgtcatcg cctcgacctg gtcgtgcgtg
     3541 acgtagatcg tcgtcgcgcc gagctcgcgg tgcagcttgg cgatctccac gcgcgtctgg
     3601 ccgcgcagcg cggcgtcgag attggacagc ggctcgtcga acaggaagac cttgggctcg
     3661 cgcacgatcg cacggccgat ggccacgcgc tgtcgctggc cgcccgacat ctcgcgcggc
     3721 agccgcttca ggtacggccc gaggttcagg atttccgccg cacggtcgac cttcttcttg
     3781 atgacggcgg gatcggcctt ggccaggcgc agcgagaagc tcatgttctc cgccaccgtc
     3841 atgtgcgggt agagcgcgta gctctggaac accatcgcca ggtcacgctg cgccgagggc
     3901 aggtgcgtga tctcgcggcc gtcgaggtgg atgctgccct cggacagcgt ctccagcccc
     3961 gcgatcagcc gcagcagcgt ggacttgccg cagcccgagg ggccgacgaa gaccgtaaac
     4021 tggccgcgat cgatggacag gtcgacgccc ttgatcgtgc ggacgtcgcc gaactgcttg
     4081 acgacgtgct tgagctcgag gaatgccatg ggaaagttcc tttacttgac cgcgccgaag
     4141 gtcagcccct gcacgagctg cttctggctg aaccagccga acaccacgat cggggcgatc
     4201 gcgagcaccg aggccgccga cagcttggcc cagaacaggc cctcggggct cgagtacgag
     4261 gcgatcagcg cggccagggt gcccgcgttc gcggcggtga ggttgagcga ccagaacgcc
     4321 tcgttccacg acagcaccag gcacagcagg ccggtcgagg caaggccgcc catcgccagc
     4381 ggcagcacga cgtggcggaa ctcggcggcc agcgaggcgc cgtccatgcg cgcggcctcg
     4441 aggatctccg gcgggatgtc cttgaagtgc gagtacagca tccacaccat cagcggcagg
     4501 ttgctgagcg tgaagacgat gatgagcccg gtgcaggtgt ccagcaggcg cgagctctgc
     4561 gccatcacgt acaccggcac gagcgcgccg accgccggca tcatcttggt ggagagcatc
     4621 cacatcagga tgtccttggt gcgcttggtc gggaagaagg ccatcgaata cgccgccggc
     4681 gccgcgatcg cgaggcccag cagcgtcgag acgacgctcg tgacgaggga gttcctcgcg
     4741 tacagcaggt agtcgctgcg ctcctgcacg tgcgtgaagt tctccagcgt cgggtggaag
     4801 atccacagcg agggcacggc gatcgcctgc agctccgtct tgaacgacgt cagcagcatg
     4861 aacaggatcg ggaagaacag cagcagcgcg acgagccacg cggcgaccgt gcgcagcgcg
     4921 aggaacagca ggccgtgggt accgggtacg gtattggcgt tcatgatcag cgccgcccgg
     4981 ccgcccgaag gcgctgagcg ccccctcggg gggcagcgaa agaagtgagc atgggggtgg
     5041 tcatttctag cgctccatgt tcttgccgac gaggcggatc aggaacgccg ccgcgatgtt
     5101 ggccagcacg accgcgaaca gcgcgcccgc cgaggccacg ccgacgtcga agccgagcag
     5161 cgcctgcttg aagatcaggt aggcgatgtt ggtgctggcg tcgccggggc cgccgcccgt
     5221 ggtggtcgag atctcggcga agacgctgag caggaagatc agctcgatca tcaccaccac
     5281 cgcgatggag cgcgcgaggt gcggcagcac gaggaagcgg aactgctgga accagttcgc
     5341 gccgtccatg cgcgcggcct ccagctgctc ccggttcatg ccctgcaacg aggtgatgaa
     5401 gatcagcacc gcgaacggca gccactgcca ggccaccatg atgatcaccg acagcagcgg
     5461 ccagtcgccg agccagtcga ccggcgtgag cccgagcgcg cgccacacct gcgcgagcac
     5521 gccgtagatc gggttcatca tcatgtgctt ccacagcagc gcgttgaccg tgggcatgac
     5581 gaagaacggc gagatcagca gcacgcgcac gatgccgcga cccgggaagg gctcgttgac
     5641 cagcagcgcg atcagcacgc ccagcaccac ggtgatcgcg atcacgctcc cgagcagcag
     5701 cagcgtgttc aggatggcgg gccagaacga cgggtcggtg atgaagaagc ggaagttctc
     5761 gaggccgacg aaggcatgct cgccgggctc catcaggttg tagcggatga ccgagaagta
     5821 cagggtcatc gccagcggca cgatcatcca gaggaagagc gagccgacgg caggcgcgag
     5881 cagcagccgg gcgagcgatc tcatggcggt ctttcgggcg agggcgtacg ggccgggaaa
     5941 ggcgcgtgcc ggcggcgtcg gtcgacgccg ccggcacgga ccgtcaggcg gtcacttcag
     6001 gcggccgctc ttcttcagct cgcgctcggc cgccacctgc gaggccttca gcgccgcgtc
     6061 gaccgactcc ttgcccgcga gggccgcgct catctgctgg ccgacggtca cgccgatgga
     6121 ctggaactcg gggatcgtcg cgaactgcac gccggtgtac gggctcttgg gcagggtcgg
     6181 gtccagcggg ttcgcgctgt cgatcgcctt cgcctcggcg gtcgcgaact tggccacggc
     6241 caggaactcg gggttcgcat aggtggactt gcgggtgcct gtcggcacgt gcgcccagcc
     6301 ttcctccttc gcgacgagct ggatgtaggc cttggaggtc gcccagttca cgaacttctg
     6361 cgcggcgtcc gcgttcttgc tgctcgcggg cacggccagg gcccatgccc acagccagtt
     6421 cgcacccttg gcggtgacct gctgcggcgc ctgcgtgaag gcgaccttgt cggcgacctt
     6481 gctctgcttc ggatcggtga tgaaggaggc cgcgatcgtc gcgtcgtccc acatcgcgca
     6541 cttgccttcg ttgtagagcg ccaggatctc gttgaagctg ttggccgagg accccggggg
     6601 gccatccttc ttcatcatgt cgacgtagaa gttgatcgcg tcgtgccacg gcttggagtc
     6661 gagctgcggc ttccacgaca tgtcgaacca ctggccgccg aacgaattga cgagcgtggt
     6721 caggaacgcc atgttgtcgc cccagcccgg cttgccgcgc aggcagatgc catagacgcc
     6781 ggccttcggg tcggtcatct tgtcggcggc ggcgcgcacc tggtcccacg tcggccggtc
     6841 ggagaaggtc acgccggcct tggcggccag gtccttgcgg tacatcagca tcgagctctc
     6901 gccgtagaac ggggcggcgt agagcttgcc gtccgccgac aggccgttgc ggatcgcggg
     6961 cagcaggtcg tcgacgtcgt agctggcgtc cggcttgatc tcctgcagcc agcccttctt
     7021 gccccagatc ggcgtctcgt acatgccgat ggtcatcacg tcgaactggc cgcccttggt
     7081 ggcgatgtcg gtcgtgacgc gctggcgcag cacgccttcc tccagcgtca cccacttcac
     7141 gtggatgtcg gggttggcct gctcgaagaa gtgggtgagc ttctgcatct cgatcatgtg
     7201 gccgttgttg acggtcgcga tcgtgatgtc ggtggcgtgg gccgggaagc tcgctgcgat
     7261 cgcgagggac gcggccaggg cgacggcccg ggccggggaa gcgttgcgcg tcatagggtc
     7321 tcctgtattt tcgtgaaggc gccgtcgact gcggcacgac gccagtgtcg gcaaatcgac
     7381 agccgtcgcg tggtactgac ggaacgaatg ccgatacttt gttgcacgaa aagtcgaggg
     7441 tttacctgga ttcgcttgcg agacaccggg cttgctgcgg tgccgcaacg gcacgtacgg
     7501 gcaacaaagt atcgacgcca gcagggtgaa cgcccgccgc gcggcgttac ggccgcgccg
     7561 cacggcgcct gcgggcgtgc agcagcgtat cgacatccgc ccgtccagta gcagcgcgct
     7621 cgcgaccgct ttcctacgct tgcaggcact tcgcggccgc tccgtaccgg cccgcggggt
     7681 cgacgtgccc atcgatcgaa cgacgaacac ttctgaagga gacacccgca tgacataccc
     7741 gatgccgtat cgaaccgccc tgctcgccgc cgccgcggcg agcgccctgc tcgcgagcgc
     7801 gccggcgcgc gccgtcgact ggaccggcta catgcgcggc ggccccgccg ccacgaccga
     7861 atccggccac tcgcgccagt gctacggcat cggcgagctc aagtaccgcc tgggcaacga
     7921 gtgcgacttc tacggcgagt tccagctcgc gcaggccatg aaggtcgacg gcgtggacgt
     7981 caacgccgtg ctgatg
//
LOCUS       DASVLN010000359         8464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_503500, whole genome shotgun sequence.
ACCESSION   DASVLN010000359 DASVLN010000000
VERSION     DASVLN010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503500"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VF453_08810"
     CDS             <1..836
                     /locus_tag="VF453_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7687794.1"
                     /translation="ALPEGAFDAGAAAVVVLALAVTLAGLWNARRPARVKTVDVPIAG
                     LPPALQGFRIAQISDLHVGPTIKRAWLERVVDAVNRLEPDVVAVTGDLVDGSVAELAE
                     HVAPLAQLRSRHGSFFVTGNHEYYSGAGPWIEHLRRLGVRVLMNEHVVLRHQQAPIVL
                     AGVADWSAHHFDPEHRSDPHRAVAGAPAAAIKVLLAHQPRSARAAADAGFHLQLSGHT
                     HGGQFLPWNFFVRFQQPFTAGLHRLKDLLVYVSRGTGYWGPPKRFGAPSEITQLRLVA
                     A"
     gene            complement(874..6705)
                     /gene="uvrA"
                     /locus_tag="VF453_08815"
     CDS             complement(874..6705)
                     /gene="uvrA"
                     /locus_tag="VF453_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7687795.1"
                     /translation="MSGLIRIRGARQHNLKNLDLDIRTGELTVVTGPSGSGKSSLVFD
                     TLYAEGQRRYVETFSAYARQFLDRMDRPAVDHVEGVPPAIAIDQTNPVRSSRSTVGTM
                     TELNDHLKLLFGRAAHLFDRETALPVRHDTPDTIYADLVARAQAAGDPRVAVTFPVEL
                     PARTTAEEVTQWLASSGFTRVHGERTVKDENGERKVLDVVADRFRVGKAEKVRVVEAI
                     ELALKRGQGRLNVDLLPEDADGPDPGVRWRWSTGLHCPESDLRYADPQPALFSFNSAF
                     GACETCRGFGRVIGVDWGLVIPDEKKTLRNGAIKAIQTPAWKEIQADLMKYAGDAGIP
                     RDTAWNHLTESQREWVIGGSPNWSGNWNKQWYGIKRFFEYLESKAYKMHIRVLLSKYR
                     SYTTCPACDGARLKLEAQLWRIGTKEQADSVLPPSKRHMPVGVSWTREQLEALPGLGL
                     HDMMLLSIDRLRRFFDQQQTPADLPDEALKLLLDEIGARLRYLNDVGIGYLTLDRQSR
                     TLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDMHRIVEAMQRLRDAGNTLVV
                     VEHDPAVMLAADRLIDMGPGPGERGGRIVFDGDPDTARNADTLTGAYLGARKQVGMGV
                     RRAVTDSTPRLILEGVREHNLRNVDVAFPLQRLVVVTGVSGSGKSTLMQDVLHPALER
                     HFGKPTETPGAHDRLLGADWLAGAVFVDQSPIGKTARSNPASYVGAFDEIRKLFATTP
                     VALERNYGAGMFSFNAGDGRCPTCGGSGFEHVEMQFLSDVYLRCPDCDGKRFRAEILD
                     AHLVRRDRQMSVADVLELTVNEAVQLFADDREVVRRLQPIVDVGLDYVKLGQPVPTLS
                     GGEAQRLKLAGFLAEAAQGPAQPAAKKGTLFLFDEPTTGLHFDDIAKLMRSLRRLLEA
                     GHSLIVIEHNLDVIRAADWLVDLGPEGGEGGGEIVGVGTPEDLKANERSHTGRALRDY
                     EEALGLERYAAVEARDKGAAYAVADAPADDAARDASRDGPSLQSLVARRRRAKPGAAD
                     ESIRIVNAREHNLKSVDVAIPRGKFTVVTGVSGSGKSTLAFDILFNEGQRRYLESLNA
                     YARSIVQPAGRPEVDAVYGIPPTVAIEQRLSRGGRKSTVATTTEVWHFLRLLWVKLGV
                     QHCINDGSPVRPQSVESIAAQLMRDYRGKHVGLLAPLVVNRKGVYTDLAKWAKARGHT
                     HLRVDGEFLKVDPWPRLDRFKEHTLELPVGDLVVEPAREAELRALLAKALDIGKGVMH
                     LLAPLDGLTEAMALEHSTLGIGAVKVFSTKRACPTCGTSYPEPDPRMFSYNSKHGWCP
                     GCVGTGLALTREQRAAFDDSVLDADERGREQAFITKEPDAEELEDATCPDCHGARLAP
                     APRAIRFEERAITDVAQWAVSDVRAWIESLQLSGRDAEVARDVIAEIRSRLVFLEDVG
                     LNYLTLDRAAPTLSGGEAQRIRLAAQLGSNLQGVCYVLDEPTIGLHPRDNRILLDALA
                     RLGAQGNTLVVVEHDEDTIRRADHLVDIGPGAGKRGGQLVAQGSAAEVAANPDSVTGR
                     CLAQPMTHPLQPRREVAFAADDAGASTHLTVIGAKLHNLQGMPVRVPLQRLVAVTGVS
                     GSGKSTLARDVLLASVADLVSSRGKAQPTVCESIEGWHAIDRVLEVDQTPIGKTPRSC
                     PATYIGFWDAIRKLFAETLEAKARGYAAGRFSFNTGGANGGRCPGCEGQGVRTIEMSF
                     LPDVKVPCDLCHGARFNPATLAVTWRGKSIGDVLQMEVDEAVEFFASMPSIAHPLQLL
                     QDVGLGYLTLGQPSPTLSGGEAQRIKLVTELSKVRDELGRRGRIDGALTPSPEGSRPP
                     GAGRRGLAPHTLYVLDEPTVGLHMADVAKLIKVLHRLTDGGHSVVVIEHDLDVVAEAD
                     WVIDLGPEGGAGGGRLVAEGTPEHLVARRTHTGLALAPVLARR"
     gene            complement(6872..8158)
                     /locus_tag="VF453_08820"
     CDS             complement(6872..8158)
                     /locus_tag="VF453_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX7687796.1"
                     /translation="MWSNWSGYVACPEAEVVTPASGAELERTVAAAVAAGRGLRLVGG
                     GHSFSPLVATDGVIVSLDGLQGVIEVDAAARTARVHAGTRLHALGDAFARHGLAMENL
                     GDINVQSIAGATSTGTHGTGRAFGNLATQIAAMKFLGADGAVHVASPDDDPALFAGGR
                     IALGSLGVLTELTLRLVPSYRLRLQRGSMALEDCLAQADALVAAHRNFEFYWLPHTDR
                     VLTKTWNLTDDPVDEMGLGRWVSDVLLENTAFGALCRLGRAVPSLCPSLSRFCASMLS
                     DSVQVDASHSMLSTVRSVRFNEMEWSLPAERGADALREIRALIDRRELPLMFPLEYRW
                     VKGDDLWLSPNFGRDSVHISVHQFVGMPWEGYFDAVQAIALNHGGRPHWGKVHSLDAA
                     RLARLYPRWDDFLALRERMDPRGRFLTPYLRRLFGV"
     gene            complement(8198..>8464)
                     /locus_tag="VF453_08825"
     CDS             complement(8198..>8464)
                     /locus_tag="VF453_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase/aldolase"
                     /protein_id="HEX7687797.1"
                     /translation="ASGPAGRDRLPRPWLPAGCALLANEGAGEVQTPVRLPAGTVLAI
                     GEPLLFRHAKAGELCERFETLLLLRGDRVVERVPTYRGEGRSFF"
BASE COUNT         1130 a         3101 c         3015 g         1218 t
ORIGIN      
        1 cggcgctgcc ggagggcgcg ttcgacgccg gggccgcggc cgtggtcgtg ctcgcgctgg
       61 cggtgacgct ggccggcctg tggaacgccc gccgcccggc gcgcgtgaag acggtggacg
      121 tgccgatcgc cggcctgccg cccgcgctgc agggctttcg catcgcgcag atctccgacc
      181 tgcacgtcgg cccgacgatc aagcgcgcct ggctggagcg cgtggtcgac gcggtcaacc
      241 gcctcgagcc tgacgtcgtg gccgtcaccg gcgacctggt cgacggcagc gtcgcggagc
      301 tggccgagca tgtcgcgccg ctcgcgcagc tgcgttcgcg ccacggcagc ttcttcgtca
      361 ccggcaacca cgagtactac tccggcgccg gcccgtggat cgagcacctg cgccgcctgg
      421 gcgtgcgcgt gctgatgaac gagcacgtgg tgctgcgcca ccagcaggcg cccatcgtgc
      481 tggccggcgt ggccgactgg tcggcgcacc acttcgatcc cgagcaccgc agcgacccgc
      541 accgcgcggt cgcgggcgcg ccggcggccg cgatcaaggt gctgctcgcg caccagccgc
      601 gcagcgcgcg cgccgccgcg gacgcgggct tccacctgca gctgtccggc cacacgcacg
      661 gcggccagtt cctgccctgg aacttcttcg tgcgcttcca gcagcccttc accgccggcc
      721 tgcaccggct gaaggacctg ctggtctacg tgagccgcgg caccggctac tgggggccgc
      781 ccaagcgctt cggcgcgccg tcggagatca cgcagctgcg cctcgtggcc gcgtgatccc
      841 cgggccgcac gacccgggga tcatccggcg gcctcacctg cgcgccagca ccggcgccaa
      901 cgccagtccg gtatgcgtcc gccgggccac gagatgctcc ggcgtccctt cggcgaccag
      961 ccgcccgccg ccggccccgc cctccggccc gaggtcgatc acccagtccg cctccgccac
     1021 gacgtccagg tcgtgctcga tcacgaccac cgagtgcccg ccgtcggtga gccggtgcag
     1081 caccttgatc agcttggcca cgtcggccat gtgcaagccc acggtcggct cgtcgaggac
     1141 gtacagggtg tgcggcgcca gcccgcgccg gcccgccccg ggcgggcggc ttccctcggg
     1201 ggaaggcgtc aaggcgccgt cgatcctgcc gcgccgcccg agctcgtcgc gcaccttgga
     1261 cagctccgtc accagcttga tgcgctgcgc ctcgccgccc gacagcgtcg gcgacggctg
     1321 ccccagcgtg aggtagccga gcccgacgtc ctggagcagc tgaagcggat gcgcgatcga
     1381 gggcatgctg gcgaagaact ccaccgcctc gtcgacttcc atctgcagca cgtcgccgat
     1441 cgacttgccg cgccacgtga ccgccagcgt cgccggattg aagcgcgcgc cgtggcagag
     1501 gtcgcagggc accttcacgt cgggcaggaa gctcatctcg atcgtgcgca cgccctggcc
     1561 ttcgcagccc gggcagcggc cgccgttcgc gccgccggtg ttgaagctga agcggccggc
     1621 cgcgtagccg cgcgccttgg cctccagcgt ctccgcgaac agcttgcgga tcgcgtccca
     1681 gaagccgatg taggtcgccg ggcaggagcg cggcgtcttg ccgatcggcg tctggtcgac
     1741 ctccagcacg cggtcgatcg catgccagcc ttcgatggat tcgcaaacag tcggttgcgc
     1801 ttttccgcgt gaggacacga ggtcggccac gctcgcgagc agcacgtcgc gcgccagcgt
     1861 cgacttgccc gagccgctga cgcccgtcac cgcgaccagc cgctgcagcg gtacccgcac
     1921 gggcatgccc tgcaggttgt gcagcttcgc gccgatcacg gtgagatgcg tgctggcacc
     1981 cgcatcgtcg gccgcgaacg cgacctcgcg ccgcggctgc agcggatgcg tcatcggctg
     2041 ggcgaggcag cggcccgtga ccgagtccgg attggccgcc acctccgccg ccgagccctg
     2101 cgcgacgagc tgcccgccgc gcttgccggc gccggggccg atgtcgacca ggtggtcggc
     2161 gcggcggatc gtgtcctcgt cgtgctcgac gaccaccagc gtgttgccct gcgcgcccag
     2221 gcgcgccagc gcgtccagca ggatgcggtt gtcgcgcgga tgcaggccga tggtcggctc
     2281 gtccagcacg tagcacacgc cctgcaggtt cgatcccagc tgcgccgcga ggcggatgcg
     2341 ctgggcctcg ccgcccgaca gcgtcggggc ggcgcggtcc agcgtgaggt agttcaggcc
     2401 gacgtcctcg aggaagacga ggcggctgcg gatctcggcg atcacgtcgc gcgcgacctc
     2461 cgcgtcgcgg ccggacagct gcagcgactc gatccatgcg cgcacgtcgc tgaccgccca
     2521 ctgcgcgacg tcggtgatgg cgcgctcctc gaagcggatc gcgcgcggcg ccggcgcgag
     2581 acgcgcgccg tggcagtcgg ggcaggtggc gtcctcgagc tcctcggcgt cgggctcctt
     2641 cgtgatgaag gcctgctcgc ggccgcgctc gtcggcatcc agcaccgagt cgtcgaacgc
     2701 ggcgcgctgc tcgcgcgtca gcgccaggcc ggtgccgacg cagccggggc accagccgtg
     2761 cttgctgttg tacgagaaca tgcgcgggtc gggctcgggg tagctcgtgc cgcaggtcgg
     2821 gcaggcgcgc ttggtcgaga acaccttgac cgcgccgatc ccgagcgtcg aatgctccag
     2881 cgccatcgcc tcggtgaggc cgtccagcgg cgcgagcagg tgcatcacgc ccttgccgat
     2941 gtccagcgcc ttggcgagca aggcacgcag ctcggcctcg cgtgcgggct cgaccacgag
     3001 gtcgccgacg ggcagctcca gcgtgtgctc cttgaagcga tccagccgcg gccacgggtc
     3061 gaccttgagg aactcgccgt ccacgcgcag gtgcgtgtgg ccgcgcgcct tcgcccattt
     3121 cgcgaggtcg gtgtagacgc ccttgcggtt gacgaccagc ggcgcgagca gtcccacgtg
     3181 cttgccgcgg tagtcgcgca tcagctgcgc ggcgatgctc tcgacgctct gcggccgcac
     3241 cgggctgccg tcgttgatgc agtgctgcac gcccagcttg acccacagca ggcgcaggaa
     3301 gtgccagacc tccgtggtcg tcgcgaccgt gctcttgcgg ccgccgcgcg acaggcgctg
     3361 ctcgatcgcc accgtcggcg ggatgccgta gaccgcatcc acctccggcc ggcccgcggg
     3421 ctgcacgatg ctgcgcgcat aggcgttgag cgactccagg tagcgccgct ggccctcgtt
     3481 gaacaggatg tcgaaggcga gcgtcgactt gcccgagccc gagacgcccg tcaccaccgt
     3541 gaacttgccg cgcgggatcg cgacgtccac ggacttcagg ttgtgctcgc gcgcgttgac
     3601 gatgcggatg gactcgtccg cggcgcccgg cttcgcgcgg cgccggcgcg cgaccagcga
     3661 ctgcaggctc gggccgtcgc ggctcgcgtc ccgcgcggcg tcgtcggccg gtgcgtccgc
     3721 gaccgcatag gccgcgccct tgtcgcgtgc ctcgaccgcg gcgtagcgct ccaggcccag
     3781 cgcctcctcg tagtcgcgca gcgcgcgtcc ggtgtgcgag cgctcgttgg ccttcaggtc
     3841 ctccggcgtg cccaccccca cgatctcgcc gccgccctcg ccgccctcgg gcccgaggtc
     3901 gaccagccag tccgcggcgc ggatcacgtc gaggttgtgc tcgatgacga tcagcgaatg
     3961 ccccgcctcc agcagccgcc gcagcgagcg catcagcttg gcgatgtcgt cgaagtgcag
     4021 gccggtcgtc ggctcgtcga agaggaacag cgtgcccttc ttcgccgccg gctgcgccgg
     4081 gccctgcgcc gcctcggcga ggaagcccgc gagcttgagc cgctgcgcct cgccgcccga
     4141 cagcgtcggc accggctggc ccagcttcac gtagtcgagg ccgacgtcga cgatgggctg
     4201 cagccggcgc accacctcgc ggtcgtctgc gaacaactgc accgcctcgt tgacggtgag
     4261 ctcgagcacg tcggccaccg acatctggcg gtcgcgccgc acgaggtggg cgtcgaggat
     4321 ctcggcgcgg aagcgcttgc cgtcgcagtc cgggcagcgc aggtagacgt cggagaggaa
     4381 ctgcatctcc acgtgctcga agccggagcc gccgcaggtc gggcagcggc cgtcgccggc
     4441 gttgaacgag aacatgcccg cgccgtagtt gcgctccagc gccaccggcg tcgtcgcgaa
     4501 cagcttgcgg atctcatcga acgcgccgac gtagctcgcc gggttcgagc gcgccgtctt
     4561 gccgatcgga ctctggtcga cgaacacggc cccggcgagc cagtcggcac cgagcaggcg
     4621 gtcgtgcgcg cccggggtct cggtgggctt gccgaagtgc cgctcgagcg ccggatgcag
     4681 cacgtcctgc atcagcgtgc tcttgcccga gccgctgacg ccggtgacga ccaccagccg
     4741 ctgcagcgga aacgcgacgt cgacgttcct gaggttgtgc tcgcgcaccc cttcgaggat
     4801 cagccgcggc gtcgagtccg tgaccgcgcg ccgcacgccc atgccgacct gcttgcgcgc
     4861 gccgaggtag gcgccggtga gcgtgtcggc gttcctcgcg gtgtcgggat cgccgtcgaa
     4921 gacgatgcgg ccgccgcgct cgcccgggcc ggggcccatg tcgatcaggc ggtccgcggc
     4981 gagcatcacg gcggggtcgt gctcgacgac gaccagcgtg ttgcccgcgt cgcgcaggcg
     5041 ctgcatggcc tccacgatgc ggtgcatgtc gcgcgggtgc aggccgatgg agggctcgtc
     5101 cagcacgaac agcgtgttca cgagcgaggt gcccagcgcg gtcgtcaggt tgatgcgctg
     5161 cacctcgccg cccgacagcg tgcggctctg gcggtcgagc gtcaggtagc cgatgccgac
     5221 gtcgttgagg tagcgcaggc gggcgccgat ctcgtcgagc agcagcttca gcgcctcgtc
     5281 gggcaggtcg gcgggcgtct gctgctggtc aaaaaaccgg cgcaggcggt cgatcgacag
     5341 cagcatcatg tcgtgcaggc cgaggccggg cagcgcctcg agctgctcgc gcgtccacga
     5401 taccccgacg ggcatgtgcc gcttcgacgg aggcagcacc gagtccgcct gctccttcgt
     5461 gccgatgcgc cacagctggg cctccagctt caggcgcgcg ccgtcgcacg cggggcaggt
     5521 cgtgtagctg cggtacttgg acagcagcac gcggatgtgc atcttgtacg ccttggactc
     5581 gaggtactcg aagaagcgct tgatgccgta ccactgcttg ttccagttgc cgctccagtt
     5641 cggcgagccg ccgatcaccc actcgcgctg cgactcggtg agatggttcc acgcggtgtc
     5701 gcgcgggatg cccgcgtcgc cggcgtactt catcaggtcg gcctggatct ccttccacgc
     5761 cggcgtctgg atcgccttga tcgcgccgtt gcgcagcgtc ttcttctcgt cggggatcac
     5821 gaggccccag tcgacgccga tcacgcgacc gaagccgcgg caggtctcgc aggcgccgaa
     5881 ggccgaattg aacgagaaca gggccggctg cgggtcggcg tagcgcaggt cgctctccgg
     5941 gcagtgcagg ccggtcgacc agcgccagcg cacgccgggg tcgggaccgt cggcgtcctc
     6001 gggcagcagg tcgacgttca ggcggccctg gccgcgcttg agcgcgagct cgatcgcctc
     6061 gacgacgcgc accttctccg ccttgccgac gcgaaagcgg tcggccacga cgtccagcac
     6121 cttgcgctcg ccgttctcgt ccttcacggt gcgctcgccg tgcacgcgcg tgaagccgct
     6181 cgaggcgagc cattgcgtga cttcctcggc cgtggtgcgc gccggcagct cgaccgggaa
     6241 cgtcaccgcg acgcgcggat cgcccgcggc ctgcgcgcgc gcgaccaggt cggcgtagat
     6301 cgtgtcgggc gtgtcgtggc gcaccggcag cgcggtctcg cggtcgaaca ggtgcgcggc
     6361 gcggccgaac agcagcttca ggtggtcgtt cagctcggtc atcgtcccga ccgtcgagcg
     6421 cgagctgcgc accgggttgg tctggtcgat cgcgatcgcc gggggcacgc cctccacgtg
     6481 gtcgacggcc ggccgatcca tgcggtcgag gaactggcgt gcataggccg agaaggtctc
     6541 gacgtagcgc cgctggccct cggcgtacag cgtgtcgaag acgaggctgg acttgcccga
     6601 gccgctcggc cccgtgacca cggtgagctc gccggtgcgg atgtcgaggt cgaggttctt
     6661 gaggttgtgc tggcgcgcgc cgcggatgcg gatgagaccg ctcatggacg tccttcccgg
     6721 ggttaggcgg gacattctag gcacgcgacg accacggcgc ggcggcgcgc cgattggccc
     6781 cggacggcgt cggacgttgc ttgaaaattc agcaggcggc cccagttccg ggccacgggc
     6841 gccaccgtcg aggtcgcgcc cgtcgccgct tctagacccc gaacaggcgc cgcaggtagg
     6901 gcgtcaggaa gcgcccgcgc ggatccatgc gctcgcgcag cgcgaggaag tcgtcccagc
     6961 gcggatacag gcgcgcgagc cgcgcggcgt ccagcgaatg caccttgccc caatgcggcc
     7021 ggccgccgtg gttcagggcg atcgcctgca ccgcgtcgaa gtagccctcc cacggcatgc
     7081 ccacgaactg gtgcaccgag atgtgcacgc tgtcgcggcc gaagttcggg ctcagccaga
     7141 ggtcgtcgcc cttcacccag cggtactcga gcgggaacat cagcggtaac tcgcgccggt
     7201 cgatcagcgc gcggatctcg cgcagcgcgt cggcgccgcg ctcggcaggc agcgaccact
     7261 ccatctcgtt gaagcgcacc gagcgcaccg tcgacagcat cgagtggctc gcgtcgacct
     7321 gcacgctgtc cgacagcatc gaggcgcaga agcggctgag cgacggacac agcgacggca
     7381 ccgcgcgccc gagccggcac agcgcgccga aggccgtgtt ctccagcagc acgtcgctga
     7441 cccagcgccc gaggcccatc tcgtcgacag gatcgtcggt gaggttccag gtcttggtga
     7501 gcacgcggtc ggtgtgcggc agccagtaga actcgaagtt gcggtgggcg gccaccagcg
     7561 catcggcctg cgcgaggcag tcctccagcg ccatgctgcc gcgctgcagc cgcagccggt
     7621 aggacggcac gaggcgcagc gtcagctcgg tcagcacgcc caggctgccg agcgcgatgc
     7681 ggccgccggc gaacagcgcg ggatcgtcat ccgggctcgc cacgtgcacc gcaccgtcgg
     7741 caccgaggaa cttcatcgcc gcgatctgcg tcgcgaggtt gccgaacgcg cggccggtgc
     7801 cgtgcgtgcc ggtgctggtc gcgccggcga tggactgcac gttgatgtcg cccaggttct
     7861 ccatcgcgag gccgtggcgg gcgaacgcgt cgcccagcgc gtgcaggcgc gtgccggcgt
     7921 gcacgcgcgc ggtgcgggcc gcggcgtcga cctcgatcac gccctgcagg ccgtcgagcg
     7981 agacgatgac gccatcggtc gccaccagcg ggctgaacga atgaccgccg ccgaccagcc
     8041 gcagcccgcg ccccgcggcg acggcggccg cgacggtgcg ctcgagctcc gcgccgcttg
     8101 cgggcgtcac gacctcggcc tcggggcatg cgacatagcc cgaccagttg ctccacatcc
     8161 tcgcgctcct cgttctggcg gtcgtcgtgc cgggtgctca gaagaagctc cggccctcgc
     8221 cgcggtaggt cggcacgcgc tccacgacgc gatcgccccg cagcagcagc agcgtctcga
     8281 agcgctcgca cagctcgccg gccttggcat ggcggaacag cagcggctcg ccgatcgcga
     8341 gcaccgtccc cgcgggcagc cgcacgggcg tctgcacctc gcccgcgccc tcgttggcga
     8401 gcagcgcgca acccgcgggc agccaggggc gcggcaggcg gtcgcgcccg gccgggcccg
     8461 aggc
//
LOCUS       DASVLN010000360         5161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_72028, whole genome shotgun sequence.
ACCESSION   DASVLN010000360 DASVLN010000000
VERSION     DASVLN010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72028"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF453_08830"
     CDS             <1..241
                     /locus_tag="VF453_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687798.1"
                     /translation="PRPSRTRRLAELALVLGATEALHLALAGGDADFGGAELAAGSLL
                     LAAWLLRHTWEALRPRQRVRTWNRPRGLDRLRPSR"
     gene            complement(260..1141)
                     /gene="kynA"
                     /locus_tag="VF453_08835"
     CDS             complement(260..1141)
                     /gene="kynA"
                     /locus_tag="VF453_08835"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEX7687799.1"
                     /translation="MGHGSPASAVPGPVSAGGERIVAEEGAQLDFARDMSYGDYLHLD
                     QVLSAQHPLSPDHNEMLFIVQHQTSELWMKLMLHELRAAVAAIATDRLGAAFKMMARV
                     SRIMEQLVHAWDVLATMTPPEYSAIRPYLSASSGFQSYQYRCIEFTMGNKNAAMLKPH
                     AHRPDLLALVEQAWRAPSLYDESLRLLARRGIPVPVSHLERDWTQPYVESDAVRDAWL
                     QVYRAPETHWDLYQLGEELTDLEDAFRLWRFRHVTTVQRIIGFKRGTGGTSGVGYLKK
                     MLDVVLFPEIWGLRTEL"
     gene            complement(1185..2501)
                     /gene="kynU"
                     /locus_tag="VF453_08840"
     CDS             complement(1185..2501)
                     /gene="kynU"
                     /locus_tag="VF453_08840"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEX7687800.1"
                     /translation="MTTRDDCARRDAEDALAPLREQFALPEGVIYLDGNSLGALPRAT
                     AGRVDAAVRQEWGEGLIRSWNDAGWITLPQRVGDRIGALLGARPGETVAADSTSVNLF
                     KVLSAAARIAHADAPQRRVIVSERQNFPTDLYIAEGLCRELGWTLELLEPPAIVERLA
                     GGRGADVAIAMLTQVNYRTGALHDMAAMTARIHGAGALAVWDLAHTAGAVEIDLHGAD
                     ADFAIGCGYKFLNGGPGAPAFVWAHPRHAERFWQPLSGWMGHAAPFAFQPGYEPAPGI
                     GRFLCGTPAVLSLTALECGVDTLHAAAPLGGMRALREKSIALTEAFIALVDARCSELG
                     VAVASPRDARARGSQVSLVHTAPDGGRHGYAIMQALIARGVIGDFRAGTGADRHGPAL
                     PDILRFGFTPLYLRFVDVYDAVEHLVQVLASGEYRDDRFNRRAAVT"
     gene            2635..5064
                     /locus_tag="VF453_08845"
     CDS             2635..5064
                     /locus_tag="VF453_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX7687801.1"
                     /translation="MRIACIGGGPAGLAFALLMKKLDPSHDITVVERNRPYDTFGWGV
                     VFSDATMAHLRRWDAETAGAIEAAFAHWDDIVLEFKGRAIRSGGHGFVGIGRKRLLNI
                     LQARCESLGVRLEFDREVRSDDEFPDADLVVASDGINSAVRQKYADLVRPDIVTRPNR
                     FIWLGTKKLYDAFTFVFERTEHGWFQAHVYRFDAGTSTFIVECTEDTWRAHRLDQLDT
                     EASVAFCERVFARHLQGEKLMSNARHLRGSAWLNFQRITCERWWATAEERAARSPGAH
                     APLVLMGDAVHTAHFAIGSGTKLALEDAIELANQFKLAAEAQGEVGPNDLPALLARYQ
                     ELRAVEVLRLQNAAWNAMEWFEVCGRRYCDQLEPEQFMYSLLTRSQRISHENLRLRDA
                     GWLEGYEQWFARRQSASEGRPGRAGLALEGRRREDRANPGVPPPMFTPFTVRSVTLKN
                     RVVVSPMAQYSAIDGTVGDYHLVHLGARAMGGAALVMTEMTCTSADARITPGCPGLYT
                     DAQRDAWARIVDWVHDHTDARIGMQLGHAGAKGATHLGWEGIDQPLTSGGWPLMSASS
                     QQYLPGVSDWSRAMTRADMDRVLHDFVAAARRAADAGFDWLELHCAHGYLLSSFISPL
                     TNRREDDYGGPLENRLRYPLQVFAAVRAAWPQQLPMSVRISTHDWVEGGITPADAVEI
                     ARAFKAAGADMIDCSSGQVSKAEKPVYGRMWQTPFADRIRNEAGIATIAVGAITEADH
                     VNAIIAAGRADLCAVARPHLANPAWTLTEAARIGYAGVQWPAPYRSAKPQLEAIFQRE
                     RAAAQATRSQP"
BASE COUNT          730 a         1864 c         1843 g          724 t
ORIGIN      
        1 gccgcgaccg tcgcgcacgc ggcggctcgc cgagctcgcg ctggtcctgg gcgcgacgga
       61 ggcgctgcac ctggcgctcg ccgggggcga cgcggacttc ggtggcgcgg agctcgccgc
      121 gggctcgctg ctgctggccg cctggctgct gcgccacaca tgggaggcat tgcggccgcg
      181 ccagcgggta cgcacctgga acaggccgcg cggcctggat cgcctgcgac cgagccgctg
      241 acgcgcggcg ccgcgccggt cagagttcgg tgcgcagccc ccagatctcg gggaacagca
      301 cgacgtcgag catcttcttg aggtagccga cgccgctcgt gccgccggtc ccgcgcttga
      361 agccgatgat gcgctgcacc gtcgtcacgt ggcggaatcg ccagaggcgg aacgcgtcct
      421 cgaggtcggt gagttcctcg ccgagctggt agagatccca gtgcgtctcc ggcgcgcggt
      481 agacctgcag ccacgcgtcg cgcacggcgt cgctctcgac gtagggctgc gtccagtcgc
      541 gctccaggtg agatacaggt accggtattc cgcggcgcgc gagcagccgc aggctctcgt
      601 cgtacagcga gggtgcgcgc caggcctgct cgaccagcgc cagcaggtcg ggccggtggg
      661 cgtgcggctt gagcatcgcc gcgttcttgt tgcccatcgt gaactcgatg cagcggtact
      721 ggtagctctg gaagccgctg ctggcggaga ggtagggccg gatcgccgag tactcgggcg
      781 gcgtcatcgt cgccagcacg tcccaggcgt gcaccagctg ctccatgatg cggctgacgc
      841 gcgccatcat cttgaacgcg gcgccgaggc ggtcggtcgc gatcgcggcg acggcggcgc
      901 gcagctcgtg cagcatcagc ttcatccaga gctcggaggt ctggtgctgc acgatgaaca
      961 gcatctcgtt gtgatccggc gacagcggat gctgcgcgct gagcacctga tcgagatgca
     1021 ggtagtcgcc gtagctcatg tcacgcgcga aatcgagctg cgcgccctcc tccgcgacga
     1081 tgcgctcgcc gcctgcagat acgggcccgg gtacggcgga cgcaggcgag ccgtggccca
     1141 tcgggcaagc gccggccgag gtgtcgtcgt tcatggggaa gtcctcaggt gaccgcggcg
     1201 cggcggttga atcgatcatc gcggtactcg cccgacgcga gcacctgcac gaggtgctcg
     1261 acggcgtcgt acacgtcgac gaagcgcagg taaagcggcg tgaagccgaa gcgcaggatg
     1321 tcgggcagcg cggggccgtg gcggtcggcg ccggtgccgg cgcggaagtc gccgatcacg
     1381 ccgcgcgcga tcaacgcctg catgatcgcg tagccgtgcc ggccgccgtc gggcgcggtg
     1441 tgcacgaggc tcacctggct gccgcgcgcg cgcgcgtcgc gcgggctcgc gaccgcgacg
     1501 ccgagctcgg agcaacgcgc gtcgacgagc gcgatgaagg cttcggtcag cgcgatcgac
     1561 ttctcgcgca gcgcccgcat cccgcccagc ggcgcggcgg cgtgcagcgt gtcgacgccg
     1621 cactcgagcg cggtgagcga cagcaccgcc ggcgtgccgc acaggaagcg gccgatgccc
     1681 ggtgccggct cgtagccggg ttggaacgcg aacggcgcgg cgtggcccat ccagccggac
     1741 agcggctgcc agaagcgctc ggcgtggcgc ggatgcgccc acacgaaggc cggcgcgccg
     1801 gggccgccat tgaggaactt gtagccgcag ccgatcgcga agtcggcgtc ggcgccgtgc
     1861 aggtcgatct ccaccgcgcc ggccgtgtgc gcgaggtccc atacggccag cgcgccggcg
     1921 ccgtggatgc gcgccgtcat cgcggccatg tcgtgcagtg cgccggtgcg gtagttgacc
     1981 tgggtcagca tggcgatggc gacatccgcg ccgcgcccgc ccgcgaggcg ctcgacgatc
     2041 gcgggcggct ccagcagctc cagcgtccag ccgagctcgc ggcagaggcc ctccgcgatg
     2101 tacaggtcgg tcgggaagtt ctggcgctcg ctcacgatca cgcggcgctg cggcgcatcg
     2161 gcatgcgcga tgcgggccgc ggcgctcagc accttgaaca ggttcaccga cgtcgagtcg
     2221 gcggccaccg tctcgcccgg acgtgcgccc agcagcgcgc cgatgcgatc gcccacgcgc
     2281 tgcggcagcg tgatccagcc tgcgtcgttc cagctgcgga tgaggccttc gccccactcc
     2341 tgccgcaccg ccgcgtccac gcgcccggcg gtcgccctcg gcagcgcgcc cagcgagttg
     2401 ccgtcgaggt agatcacgcc ctcgggcagc gcgaactgct cgcgcagcgg ggccagtgcg
     2461 tcctcggcgt cgcgccgcgc gcagtcgtct ctcgtcgtca tgcgtccggt ctccttgcgt
     2521 cgcggccgct tgcgggccgt cacgcgatta gtttagtatt gaaatattca cttctcaagt
     2581 tttgtcgcac gcatcgcgcc cggcgcatgc accgcggcgg gacacgggga tcccatgcgc
     2641 atcgcctgca tcggcggcgg cccggcgggc ctggccttcg ccctcctgat gaagaagctc
     2701 gacccgtccc acgacatcac cgtggtcgag cgcaaccgcc cctacgacac cttcggctgg
     2761 ggcgtcgtgt tctcggacgc gacgatggcg cacctgcgcc gctgggacgc cgagacggcc
     2821 ggcgcgatcg aggccgcctt cgcgcactgg gacgacatcg tgctcgagtt caagggtcgg
     2881 gcgatccgct cgggcggcca cggcttcgtg ggcatcggcc gcaagcgcct gctcaacatc
     2941 ctgcaggcgc gttgcgagtc gctgggcgtg cggctcgagt tcgaccgcga ggtgcgctcg
     3001 gacgacgagt tccccgacgc cgacctcgtc gtcgcgagcg acggcatcaa ttcggccgtg
     3061 cgccagaagt acgccgacct cgtccggccg gacatcgtga cccgcccgaa ccgcttcatc
     3121 tggctgggga cgaagaagct ctacgacgcg ttcaccttcg tcttcgagcg caccgagcac
     3181 ggctggttcc aggcccacgt ctaccgtttc gatgccggca cctcgacctt catcgtcgag
     3241 tgcaccgagg acacctggcg cgcgcaccga ctggaccagc tcgataccga agcctcggtc
     3301 gcgttctgcg agcgcgtgtt cgcgcgtcat cttcagggcg agaagctcat gagcaacgcg
     3361 cgccacctgc gcggctcggc ctggctgaac ttccagcgca tcacctgcga gcgctggtgg
     3421 gcgaccgccg aggagcgcgc cgcgcgcagc cccggcgcgc acgcgccgct ggtgctcatg
     3481 ggcgacgcgg tgcacaccgc gcacttcgcg atcggctcgg gcaccaagct cgcgctcgag
     3541 gacgcgatcg agctcgccaa ccagttcaag ctggccgccg aagcccaggg cgaggtcggg
     3601 cccaacgacc tgccggcgct gctcgcgcgc taccaggagc tgcgcgccgt cgaggtgctg
     3661 cgcctgcaga acgccgcctg gaacgcgatg gagtggttcg aggtctgcgg ccggcgctac
     3721 tgcgaccagc tcgagccgga gcagttcatg tactcgctgc tcacgcgcag ccagcgcatc
     3781 agccacgaga acctgcgcct gcgcgacgcc ggctggctgg agggctacga gcagtggttc
     3841 gcgcgacggc aatcggcgag cgagggccgc ccgggccggg ccgggcttgc cctcgagggg
     3901 cggcggcgcg aggaccgggc gaacccgggc gttccgccgc cgatgttcac gcccttcacg
     3961 gtgcgctcgg tcacgctgaa gaatcgcgtc gtcgtctcgc cgatggcgca gtactcggcg
     4021 atcgacggca ccgtcggcga ctaccacctc gtgcatctcg gggcccgcgc gatgggcggc
     4081 gcggcgctgg tgatgacgga gatgacctgc accagcgccg atgcccgcat cacccccgga
     4141 tgccccggcc tgtacaccga cgcgcagcgc gacgcctggg cgcgcatcgt cgactgggtg
     4201 cacgaccata cggatgcccg tatcggcatg cagctcggcc acgcgggcgc caagggcgcg
     4261 acccacctcg gctgggaagg catcgaccag ccgctgacct ccggcggctg gccgctgatg
     4321 tcggcatcgt cgcagcagta cctgccgggg gtgagcgact ggtcgcgcgc gatgacgcgc
     4381 gccgacatgg accgcgtgct gcacgacttc gtcgccgccg cccgccgcgc cgcggacgcg
     4441 ggcttcgact ggctcgagct gcactgcgcg cacggctacc tgctgtcgtc gttcatctcg
     4501 ccgctgacga accggcgcga ggacgactac ggcggcccgc tcgagaaccg gctccgatac
     4561 ccgctccagg tattcgccgc cgtgcgcgcc gcatggccgc agcagctgcc gatgtcggtg
     4621 cgcatctcca cgcacgactg ggtcgagggc ggcatcacgc cggccgacgc ggtcgagatc
     4681 gcgcgtgcgt tcaaggccgc cggcgccgac atgatcgact gctcctcggg ccaggtgagc
     4741 aaggccgaga agccggtcta cggccgcatg tggcagacgc ccttcgccga ccgcatccgc
     4801 aacgaggccg gcatcgcgac gatcgccgtc ggcgcgatca ccgaggccga ccacgtcaac
     4861 gccatcatcg ccgccggccg cgccgacctg tgcgccgtcg cgcggccgca cctggccaat
     4921 cccgcgtgga cgctgaccga ggccgcgcgc atcggctacg cgggcgtgca gtggccggcg
     4981 ccctaccgtt cggccaagcc gcagctggag gcgatcttcc agcgcgagcg cgccgccgcg
     5041 caggccactc ggagccagcc atgagcgaca tccgcaacgg cgacatgagc gaggtcgcac
     5101 aggtgctggg cgccatcgag ctgcccgcga ccaccgcgcc ggcggcgccc gcgacgcagc
     5161 c
//
LOCUS       DASVLN010000361        16048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_232983, whole genome shotgun sequence.
ACCESSION   DASVLN010000361 DASVLN010000000
VERSION     DASVLN010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232983"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF453_08850"
     CDS             <1..196
                     /locus_tag="VF453_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687802.1"
                     /translation="VDATLGAIARPARVRFVAGLPKTRSGKLLRRAIQAVCEGREPGD
                     LTTIDDPAALGQVRAALAME"
     gene            310..1218
                     /locus_tag="VF453_08855"
     CDS             310..1218
                     /locus_tag="VF453_08855"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX7687803.1"
                     /translation="MDATALTALPELGGEQLLALTELDGKALHALVRDIGIPARPQML
                     VDLQRELERDDPSLKKVADISGSDVALSAALLKMANSPLMGLSRRCETVEQAFQLLGF
                     AQCQAIFTEICLRKLLPADGPGLVRFWDVSAKRARAMTFLARTRRVVPPALAHTYGLF
                     VEIGIAILLQRFPGKGGYLATLDRSNAHEGTITALEQQVHGVDHALVGALTARTWGVS
                     QTAVLATRLHHEYSSWMGPMPPQVRELVALGLVCETIIQRYQGMNRHLEWVKGGEIAL
                     AALGIGEAELEDWCEDVHAQFASTGL"
     gene            1322..2065
                     /gene="ypfH"
                     /locus_tag="VF453_08860"
     CDS             1322..2065
                     /gene="ypfH"
                     /locus_tag="VF453_08860"
                     /inference="COORDINATES: protein motif:HMM:NF008525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEX7687804.1"
                     /translation="MPDDDSLIIARPEGQAQQLILLFHGVGAAPDDLAPLGHLLASEF
                     PQAFVVSVAAPDRVPGGAGRQWFAVAGIDEDSRVERVAAAMPAFATAVESWRREAGVP
                     TDAVALVGFSQGGIMALESTREGPALAGRVVAIASRFARLPQAPNPGTTLHLFHGKLD
                     AVIPYGFTVEAAQHLVAIGADVTADVVPFVGHQINEDIAALLIERLRGHVPRRVWDAA
                     MNAARELREQEGDPPGEPDAPREGPHGAA"
     gene            2198..2437
                     /locus_tag="VF453_08865"
     CDS             2198..2437
                     /locus_tag="VF453_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687805.1"
                     /translation="MHTNATLPRLDATLQDALLTTAEALARRRACDIAEATIDRLVAL
                     RWLEWAGGSLRFTAAGELVLLKAQAAMARDGLQAA"
     gene            complement(2528..3343)
                     /locus_tag="VF453_08870"
     CDS             complement(2528..3343)
                     /locus_tag="VF453_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7687806.1"
                     /translation="MRRPDPFTIRAWQLILLLALFLAWHLLTTPGLLPHFLFSDDNQA
                     AFFFGQPLKIFARIRDWFSGAEIWRHLGVTLAETVLAFAIGTVTGLATGLWLALSPVA
                     AAILDPYIKAANAMPRVILAPIFAVWFGLGIASKVALGVTLVFFIVFFNVYQGVKEVS
                     PVILANARMLGANRRQLLRHVYLPSATSWVFSSLHTSVGLAFVGAVVGEYLGSSQGVG
                     YLILQAEGSFDIDTVMAGILVLTAFALALDALVGVAEKALMKWQPKAGESEKL"
     gene            complement(3353..4177)
                     /locus_tag="VF453_08875"
     CDS             complement(3353..4177)
                     /locus_tag="VF453_08875"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7687807.1"
                     /translation="MGDAAAPPPALALVGASCTFAARTDPSQRYTAVRDVSVAVGAGE
                     FVSVVGPTGCGKSTLLNMAAGLLAPSTGQVRVFGAPLQGLNARAGYLFQAESLMPWRT
                     ARENVMAGLQFRGAGRDAAGAQADEWLKRVGLAAFGDRYPHQLSGGMRKRVSLAQVLV
                     LDPDIILMDEPFSALDIQTRQLMENEVLDLWSAKRKAVLFITHDLDEAIAMSDRVVVM
                     SAGPASHPIGEFRIDLPRPRDVAEVRAHPRFVELHRAIWDVLREEVLAGYQQQLKA"
     gene            complement(4177..5196)
                     /locus_tag="VF453_08880"
     CDS             complement(4177..5196)
                     /locus_tag="VF453_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7687808.1"
                     /translation="MQRRSLVALAAAAVAAPAFVRAQSLERPQLTIAVGGKNLLYYLP
                     LTIAETLGYFKAEGLDVTIVDFAGGSKALQAVVGGSADVVSGAFEHTLLMQAKGIRLR
                     AFVLQGRAPQVVLGINPKTMPNYRSVADLRGKKIGVTAPGSSTNILANFVLAKAGVKP
                     SEVSIIGVGAGPGAVAAMRAGQVDAISNLDPVITLLQRSGDLKIVSDTRIVSVADAAF
                     GGPMPAGCLYAPQAFIDKDPHTVQALANAIVRADRWIQQAGPADIVKAVPESYLLGDR
                     AVYIDSFLASKGALSSDGMIPEAGAATALRALQSVDDALKGGKFDLAAAWTNEFAKRA
                     NAKKA"
     gene            complement(5315..6106)
                     /gene="folE2"
                     /locus_tag="VF453_08885"
     CDS             complement(5315..6106)
                     /gene="folE2"
                     /locus_tag="VF453_08885"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEX7687809.1"
                     /translation="MNSLTDVLNIPDTQSEHDDRHLAIQLVGVKRVRYPLQLRVAGAA
                     QQTAAEWSLAVALPADQKGTHMSRFVAWLDQLDGPMDAAMLEAAFADMLQRLHADEGR
                     VEAAFTFFLRKSAPVSGISSLLDYQGRWIAERKDGRTSVWAEVTVPVKTLCPCSKEIS
                     DYGAHNQRSAVTIRAELKSPVEWHELVRFAEDSASCELWPLLKRADEKWVTEHAYENP
                     KFVEDLVRDVAIAVQREPRIGRYAIDVENFESIHNHSAYARIERP"
     gene            complement(6280..8169)
                     /gene="dxs"
                     /locus_tag="VF453_08890"
     CDS             complement(6280..8169)
                     /gene="dxs"
                     /locus_tag="VF453_08890"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX7687810.1"
                     /translation="MTTNLLATIASPADLRRLSRAELRTLCDELRQYVLHSVSKTGGH
                     LSSNLGTVELTVALHYVFNTPHDRLVWDVGHQTYPHKVLTGRRDAMPTLRQYGGLSGF
                     PKRDESEYDAFGTAHSSTSISAALGMAHAAKLKGEDRKAVAIIGDGAMTGGMAFEALN
                     NAGVSVADMLVILNDNDMSISPPVGALNRYLARLMSGKFYAKAREGAKNVLKNTPLYE
                     FARRFEEHTKGMVVPGTIFEEFGLNYVGPIDGHDLDSLIPTLENLRDAKGPQFLHVVT
                     RKGMGYKLAEADPVNYHGPGKFDVAVGLQKPATPPKQTFTQVFGHWLCDMAAADKRLV
                     GITPAMREGSGMVEFEQRFPERYHDVGIAEQHAVTFAAGLACEGLKPVVAIYSTFLQR
                     GYDQLIHDVAIQNLPVVFALDRAGIVGADGPTHAGAYDIAYIRCVPNLSLIAPADERE
                     TRQALSTAFGQGHPVAVRYPRGAGAGVAPLASLEGFAWGKAETRRTGRRIAILAFGTL
                     LYPALEAAEKLDATVVNMRFIKPLDVDRVLELARTHEALVTIEEGCIMGGAGSAVLEA
                     LAAAGVAVPVLQLGLPDRFVEHGDPVRLLHELGLDAEGIEHAVRRRFGPSLAVVRGGS
                     AAAAG"
     gene            complement(8208..9095)
                     /locus_tag="VF453_08895"
     CDS             complement(8208..9095)
                     /locus_tag="VF453_08895"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX7687811.1"
                     /translation="MKIDDFARWVRARQDDVERALERWVPAGAPAELGLVMRYAVLDG
                     GKRLRPLLVMAAAEAVGGDLDAALRAAVAVELIHAYSLVHDDMPCMDNDVLRRGKPTV
                     HVKYGEAQAMLAGDAMQALAFDVLTPDEGLAPELMARLVRMLARASGHDGMAGGQAID
                     LASVGRCLDEAALRDMHRRKTGVLLQASVMMGAACGPASTAALAALSDYGAAVGLAFQ
                     VVDDVLDVTQASEVLGKTAGKDVDQNKPTFVSLLGLEQAQRYALDLRDQAHAALARSG
                     LGQAAYLACLADKIVERDN"
     gene            complement(9092..9331)
                     /gene="xseB"
                     /locus_tag="VF453_08900"
     CDS             complement(9092..9331)
                     /gene="xseB"
                     /locus_tag="VF453_08900"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7687812.1"
                     /translation="MDRTDAPTPADAPASFEQAQAELDELVRAMESGQLPLDGLLGAY
                     RRGAQLLEFCRAKLDAVEEQVKVLEEGQLKPWTRT"
     gene            9661..10779
                     /locus_tag="VF453_08905"
     CDS             9661..10779
                     /locus_tag="VF453_08905"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX7687813.1"
                     /translation="MSDLSITLEALERARSQLSVSTYFDEDLFRREQELIFQHGPRYL
                     AHELAVPEIGDYYALPQEGEGRVLMRGHDGGVQLLSNVCRHRQAVMLRGRGQTASNIV
                     CPLHRWTYGLDGRLIGAPHFDEDPCLHLNRYRTRSWNGLVFEDNGRDVAADLAALGPR
                     AALDFGGYVLDKVHVHQCDYNWKTFIEVYLEDYHVGPFHPGLGGFVSCDDLQWEFGTN
                     YSVQTVGVADRLARPGSATYRKWHDAVLAYNGGVAPRHGAIWLTYFPNIMVEWYPHCL
                     VVSTLYPKGPQQTTNVVEFYYPEEIAAFERELVEAEQAAYLETCVEDDEIALRMDAGR
                     KALFERGDDEAGPYQSPMEDGMRHFHEWYRRAMQQPAR"
     gene            10861..11760
                     /locus_tag="VF453_08910"
     CDS             10861..11760
                     /locus_tag="VF453_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7687814.1"
                     /translation="MSASLLMVCSTFLFAAMGVCVKLASTRYGTGEIVMYRGLVGMVG
                     IAMVARSRGVGLRTRVPMMHAWRGIVGVTALALWFHAIAHLPLATAVTLNYTSSVWMA
                     LFLIGGAVVAGAQRVDPRLVALVLVGFGGVALVLHPTLARDQLAPGVIGLASGMLSAL
                     AYLQVTTLGRVGEPELRVVFYFSMAGFLAGVVATAVEGAHLPTLADAAQLLGIGVFAT
                     SAQVAMTRAYAIGRPLSNASLQYLGIAFSFIFGVVLFHDPVTLSALGGMALIVATGLG
                     ASLLRARTLAATAPDTLPPPQDS"
     gene            11762..12625
                     /locus_tag="VF453_08915"
     CDS             11762..12625
                     /locus_tag="VF453_08915"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX7687815.1"
                     /translation="MPDTPTAFALPVPLVSARALMDALAVGAPVVLVDCSFDLADTGA
                     GERAFERGHLPGAAYAHLDRDLAGVKTGRNGRHPLPARVDWATTLGRLGATPARHVVA
                     YDAQGGMYAARAWWMLRWAGHAAVSVLDGGLQVWTAAGGALESGPSTVADEGAYPIAA
                     SLVPVIEADALQKSLGRVTLLDARAGERFRGEVEPLDARAGHIPGARSHFFKGNLGPD
                     GRFRSPEALRADFEAYGAAPQEVVHQCGSGVTACHNLLAMEIAGLPGASLYPGSWSEW
                     SSDPSRPVAVG"
     gene            12769..13203
                     /locus_tag="VF453_08920"
     CDS             12769..13203
                     /locus_tag="VF453_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7687816.1"
                     /translation="MYRSILIPTDGTEVTAKAVATAVELARLMGARLSTITVKEPFPY
                     SAISEMQPIPPQEFYDAQERIALRRVHDVVAAASAAGLACEAFTVEAVHPWEAILDHA
                     KTQGCDLIVMASHGRRGVGALLLGSETTRVLTHCQLPVLVVR"
     gene            complement(13204..14041)
                     /locus_tag="VF453_08925"
                     /pseudo
     CDS             complement(13204..14041)
                     /locus_tag="VF453_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
     gene            14166..15668
                     /locus_tag="VF453_08930"
     CDS             14166..15668
                     /locus_tag="VF453_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX7687817.1"
                     /translation="MASTTPPAVGPLAAIDMGSNSFRLEIGQVQEGRYRRHLYLKETV
                     RLGAGLDVDGMLTEEATERGLKCLATFAETLNTSAPMQVRAVATQTLREARNRDTFLV
                     RAEAALGRPIEIISGREEARLIYAGVAFLQPATKPRLVVDIGGRSTEMILGNGRAPRL
                     AESFQVGCVSLSMRYFGDGRYTRAAFRAAQVAAGAELEEGLVPFARRHWREALGSSGT
                     AGAVSDLLRANGKTDGRITPEGLRWLIERCIECGHIDKLDIPGLKPERRAVIAGGLAI
                     LYTLAAQFGIEELLPAKGALRQGVIVDLSERLAASHEAVGHDLRDSTIKELQHRFHVD
                     VAQAGRVGEAARRLFDDVCQDADAECQRELRWAAALHEAGLMVSHHDHHRHSAYIIAH
                     VDAPGFSQSQQRRLAELVLGQRGGLRKIEAALARPLTPWQVLCLRLAVIECHARGQVD
                     ARGLKLRRVGTEAHLSFTPEWAEAHPRTAYLLREEAALWARQGVLLLKLD"
BASE COUNT         2266 a         5733 c         5741 g         2308 t
ORIGIN      
        1 cgtcgacgcg acgctcggcg cgatcgcccg gccggcgcgc gtgcgcttcg tcgcggggct
       61 gccgaagacg cgctcgggca agctgctgcg gcgggcgatc caggcggtgt gcgagggccg
      121 cgagccgggc gacctcacga ccatcgacga tcccgccgcc ctcggccagg tgcgcgcagc
      181 gcttgcgatg gagtgagcgc tcgcggatga tggcgggccg cgccgcgcga tcggcgtgcc
      241 gccgctcaga gttcccgcga atctgccgat aagccggtgt cagccccgga tcacaggatg
      301 ccgcctcgca tggacgccac cgccctcacc gcgctgcccg aactcggcgg cgaacagctg
      361 ctcgccctca ccgaactcga tggcaaggcc ctgcatgcgc tggtgcgcga catcggcatc
      421 ccggcgcggc cgcagatgct ggtcgacctg cagcgcgagc tggagcgcga cgacccctcg
      481 ctgaagaagg tggccgacat ctccggctcg gacgtcgcgc tgtcggcggc cctgctgaag
      541 atggccaact cgccgctgat gggcctgagc cgccgctgcg agaccgtgga acaggcgttc
      601 cagctgctcg gcttcgcgca gtgccaggcg atcttcacgg agatctgcct gcgcaagctg
      661 ctgcccgccg acgggccggg cctggtgcgc ttctgggacg tctcggccaa gcgcgcacgg
      721 gcgatgacct tcctcgcccg cacgcgccgc gtcgtgccgc cggcgctcgc gcacacctac
      781 ggcctgttcg tcgagatcgg catcgcgatc ctgcttcaac gcttccccgg caagggcggc
      841 tacctggcga cgctggaccg ctccaacgcc cacgagggca cgatcacggc gctcgagcag
      901 caggtgcacg gcgtcgacca cgcgctcgtc ggtgcgctga cggcgcgcac ctggggcgtg
      961 tcgcagacgg ccgtgctcgc cacgcgcctg caccacgagt acagctcctg gatgggcccg
     1021 atgccgccgc aggtgcggga gctggtcgcg ctcggcctcg tctgcgagac catcatccag
     1081 cgctaccagg gcatgaaccg ccacctcgag tgggtcaagg gcggcgagat cgcgctcgcc
     1141 gcgctgggca tcggcgaggc cgagctcgag gactggtgcg aggacgtgca cgcgcagttc
     1201 gcgtcgaccg ggctctgaaa ccccgcgatg ccgggcgcgg gcggcgacgc cgggcgcgcg
     1261 cgcccgccgg tcgcgggcat ggttcaatga ggccgttggc gccccgcgcc ggagacctcg
     1321 catgcccgac gacgactcgc tcatcatcgc ccgccccgag gggcaggccc agcagctgat
     1381 cctgctcttc cacggcgtgg gcgccgcgcc ggacgacctg gcgccgctcg gccacctgct
     1441 cgcgtccgaa ttcccgcagg ccttcgtcgt cagcgtggcg gctcccgacc gcgtccccgg
     1501 tggcgcgggc cggcagtggt tcgcggtggc gggcatcgac gaggacagcc gtgtcgagcg
     1561 cgtcgccgcc gcgatgccgg cgttcgcgac ggcggtcgag tcctggcgcc gcgaggccgg
     1621 cgtgccgacc gacgcggtcg cgctggtcgg cttctcgcag ggcggcatca tggcgctgga
     1681 gtcgacacgc gaggggccgg cgctggccgg gcgcgtcgtc gcgatcgcgt cgcgcttcgc
     1741 ccggctgccc caggcgccca acccgggcac cacgctgcac ctgttccatg gcaagcttga
     1801 cgcggtcatc ccctacggct tcaccgtcga ggccgcgcag cacctggtgg cgatcggcgc
     1861 ggacgtcacg gccgacgtcg tgcccttcgt cggccaccag atcaacgagg acatcgccgc
     1921 cctgctgatc gagcgcctgc gcggccacgt gccgcggcgg gtgtgggacg cggcgatgaa
     1981 cgcggcgcgc gagctgcggg agcaggaggg cgacccgccg ggcgagcccg acgcgccgcg
     2041 cgaagggccg cacggcgcgg cctgagaccg gtcgcggcgg ggcccgcagg gtggcgtccc
     2101 gcgcgccggc ctcgtcctac atcgcgacgc tgcggacgcc gacttccggc gctccggcgc
     2161 gtgggcaaca tgcgctccat cgacggagac acgcaccatg cacaccaacg ccacccttcc
     2221 gcgcctggac gccaccctgc aggacgccct gctcaccacg gccgaggcgc tcgcccgccg
     2281 ccgcgcctgc gacatcgccg aggccacgat cgatcgcctg gtcgcgctgc gctggttgga
     2341 atgggccggc ggctcgctgc gcttcaccgc ggccggcgag ctggtgctgc tgaaggccca
     2401 ggccgcgatg gcgcgcgacg ggctgcaggc cgcctgaagg gcgcgcgccc ggcctgccat
     2461 cctgcgcagc cgcggccgac gaggccttca gtccggacac gcgacccgcc gcgcgccggg
     2521 cacgccgtca cagcttctcg ctctcgcccg ccttgggctg ccacttcatc agcgccttct
     2581 cggccacgcc cacgagcgcg tccagcgcca gcgcgaaggc cgtgagcacc aggatgccgg
     2641 ccatcacggt gtcgatgtcg aagctgccct ccgcctgcag gatcaggtag cccacgccct
     2701 gcgacgagcc gaggtactcg cccacgaccg cgccgacgaa cgcaagtccc accgaggtgt
     2761 gcagcgagct gaagacccag ctcgtggcgc tcggcaggta gacgtggcgc agcagctggc
     2821 ggcggttcgc gcccagcatg cgcgcgttgg cgaggatcac cgggctcacc tccttcacgc
     2881 cctggtagac gttgaagaag acgatgaaga acaccagcgt gacgccgagc gccaccttgc
     2941 tcgcgatgcc gaggccgaac cagaccgcga agatcggcgc caggatcacg cgcggcatcg
     3001 cgttcgcggc cttgatgtag ggatcgagga tggccgcggc gacggggctc agcgcgagcc
     3061 acagcccggt cgcgagcccg gtcacggtgc cgatcgcgaa ggccagcacc gtctcggcga
     3121 gcgtcacgcc gagatggcgc cagatctcgg cgccggagaa ccagtcgcgg atgcgcgcga
     3181 agatcttcag cggctggccg aagaagaacg cggcctggtt gtcgtcggag aacaggaagt
     3241 gcggcagcag gcccggcgtc gtgagcaggt gccaggcgag gaacagggcg agcagcagga
     3301 tcagttgcca ggcgcggatc gtgaaggggt cggggcggcg catgtcgggc cctcacgctt
     3361 tcagttgctg ctggtagccg gcgaggacct cctcgcgcag cacgtcccag atcgcgcggt
     3421 gcagctcgac gaagcgcgga tgcgcgcgca cctcggccac gtcgcgcggg cgcgggaggt
     3481 cgatgcggaa ctcgccgatc ggatgcgagg ccgggcccgc ggacatcacg acgacgcggt
     3541 cgctcatcgc gatggcttcg tcgaggtcgt gcgtgatgaa cagcaccgct ttcctcttgg
     3601 cgctccagag gtccagcacc tcgttctcca tcagctgccg cgtctggatg tcgagggccg
     3661 agaacggttc gtccatcagg atgatgtcgg gatcgagcac cagcacctgc gcgaggctga
     3721 cgcgcttgcg catgccgccc gagagctggt gcggatagcg gtcgccgaac gccgcgaggc
     3781 cgacccgctt gagccactcg tcggcctgcg cccccgccgc gtcgcgtccg gcgccccgga
     3841 actgcaggcc cgccatcacg ttctcgcgcg cggttcgcca cggcatcagg ctttcggcct
     3901 ggaacaggta gccggcgcgc gcgttcaggc cctgcagggg cgcgccgaac acgcgtacct
     3961 ggcccgtgct gggcgcgagc aggccggcag ccatgttcag cagcgtgctc ttgccgcagc
     4021 cggtcggccc gacgacggag acgaactcgc ccgcgccgac ggcgactgag acgtcgcgca
     4081 cggcggtgta gcgctgcgag ggatcggtgc gcgcggcgaa ggtgcagctc gcgcccacga
     4141 gcgccagggc ggggggcggc gcggcggcgt cgcccatcag gccttcttcg cgttcgcgcg
     4201 cttcgcgaac tcgttcgtcc aggcggccgc gaggtcgaac ttgccgccct tgagcgcgtc
     4261 gtccacgctc tgcagcgcgc gcagcgcggt cgcggcgccg gcctcgggga tcatgccgtc
     4321 ggacgacaac gcgcccttgc tcgcgaggaa cgagtcgatg tagacggccc ggtcgcccag
     4381 caggtagctc tcgggcacgg ccttgacgat gtcggccggc ccggcctgct ggatccacct
     4441 gtccgcacgc acgatcgcat tggccagcgc ctgcacggtg tgcggatcct tgtcgatgaa
     4501 cgcctgcggc gcgtacaggc agccggccgg catcgggccg ccgaaggccg cgtcggcgac
     4561 cgagacgatg cgcgtgtccg agacgatctt caggtcgccg ctgcgctgca gcagcgtgat
     4621 gacggggtcg aggttgctga tcgcgtcgac ctggccggca cgcatcgcgg cgaccgcgcc
     4681 cgggcccgcg cccacgccga tgatggacac ctccgacggc ttcacgccgg ccttggcgag
     4741 cacgaagttg gcgaggatgt tggtcgacga gcccggcgcc gtgacgccga tcttcttgcc
     4801 gcgcaggtcg gcgaccgacc ggtagttcgg catcgtcttc gggttgatgc ccagcaccac
     4861 ctgcggcgcg cggccctgga gcacgaaggc gcgcagccgg atgcccttgg cctgcatcag
     4921 cagcgtgtgc tcgaacgccc ccgagaccac gtccgcgctg ccgcccacga ccgcctgcag
     4981 ggccttggag ccgcccgcga agtcgacgat cgtcacgtcc aggccctcgg ccttgaagta
     5041 gccgagcgtc tcggcgatcg tcaagggcag gtagtacagc aggttcttgc cgccgacggc
     5101 gatcgtcagc tggggccttt ccagcgactg tgcgcgcacg aatgcgggcg ctgccacggc
     5161 cgccgcggcc agggcgacga gggaacgtcg ttgcatgagg gctccttcgt ttgggagagc
     5221 ccactttacc cacctacgcc gctgtgcgca gcgggcaaga cccgggcttg ccggatcgcg
     5281 agcgcagcgg cccgcgccgc gaagcctcgc ggcctcaggg tcgctcgatg cgggcgtagg
     5341 cggagtggtt gtggatcgac tcgaagttct cgacgtcgat cgcgtagcga ccgatgcgcg
     5401 gctcgcgctg gacggcgatc gccacgtcgc gcacgaggtc ctcgacgaac ttcgggttct
     5461 cgtaggcgtg ctcggtgacc cacttctcgt cggcgcgctt gagcagcggc cacagctcgc
     5521 acgaggcgct gtcctcggcg aaacgcacga gctcgtgcca ttcgaccggc gacttcagct
     5581 cggcgcggat cgtgaccgcc gagcgctggt tgtgcgcgcc gtagtccgag atctccttgg
     5641 agcaggggca cagcgtcttc acgggcaccg tgacctcggc ccagaccgag gtgcggccgt
     5701 ccttgcgctc ggcgatccag cggccctggt agtccagcag gctggagatg cccgagaccg
     5761 gcgcgctctt gcgcaggaag aacgtgaacg cggcctcgac gcgcccctcg tcggcgtgca
     5821 ggcgctgcag catgtcggcg aacgcggcct ccagcatcgc ggcgtccatc ggaccgtcga
     5881 gctggtcgag ccaggccacg aagcgcgaca tgtgcgtgcc cttctggtcg gccggcagcg
     5941 cgaccgcgag gctccactcc gccgccgtct gctgcgccgc gccggccacg cgcagctgca
     6001 gcggatagcg cacgcgcttg acgcccacga gctggatcgc gagatggcgg tcgtcgtgct
     6061 cgctctgggt gtcggggatg ttgaggacgt cggtcagcga attcatgggt cggggccgga
     6121 ggccgggtgc cggacgaagc gggtggcgga tggccggtgc cgcgcgcggc gatccggggc
     6181 tgcaagcatg ccaccggcag attgggactg ccgccgaaag ggcaagctgt ccagttggcg
     6241 acaccggtgc cgaggcaccg tgtcgacgcg cccgccttct cagccggccg cagccgcgga
     6301 gccgccgcgc acgacggcca gcgacggccc gaagcggcgg cgcacggcgt gctcgatgcc
     6361 ctcggcgtcg aggccgagct cgtgcaggag ccgcaccgga tcgccgtgct cgacgaagcg
     6421 gtcgggcagg cccagctgca gcaccggcac cgcgacgccc gcggcggcca gcgcctccag
     6481 caccgcgctg ccggcgccgc ccatgatgca gccttcctcg atcgtgacga gcgcctcgtg
     6541 cgtgcgcgca agctcgagca cgcgatcgac gtccagcggc ttgatgaagc gcatgttcac
     6601 gacggtggcg tcgagcttct cggccgcttc cagtgccggg tagagcagcg tgccgaaggc
     6661 gaggatcgcg atgcgacggc ccgtgcgccg cgtctcggcc ttgccccagg cgaagccctc
     6721 gagcgaggcg agcggcgcga cgcccgcgcc cgcgccgcgc gggtagcgca cggccacggg
     6781 atggccctgc ccgaacgcgg tgctcagcgc ctggcgggtc tcgcgctcgt cggccggcgc
     6841 gatcaggctc aggttcggga cgcagcggat gtaggcgatg tcgtaggcgc ccgcgtgcgt
     6901 cggaccatcg gcgccgacga tcccggcgcg gtccagcgcg aagacgaccg gcaggttctg
     6961 gatggcgacg tcgtggatca gctggtcgta gccgcgctgc aggaacgtcg aatagatcgc
     7021 cacgaccggc ttcaggccct cgcaggcgag gccggcagcg aaggtgactg cgtgctgctc
     7081 ggcgatgccg acgtcgtggt agcgctcggg gaaacgctgc tcgaattcca ccatgcccga
     7141 gccttcgcgc atcgcgggcg tgatgccgac gaggcgcttg tcggccgcgg ccatgtcgca
     7201 cagccagtgg ccgaagacct gcgtgaaggt ctgcttgggc ggcgtcgcgg gcttctgcag
     7261 gccgaccgcg acgtcgaact tgcccgggcc gtggtagttg accgggtcgg cctccgcgag
     7321 cttgtagccc atgccctttc tcgtcaccac gtgcaggaac tgcgggccct tggcgtcgcg
     7381 caggttctcc agcgtcggga tcagcgagtc gaggtcgtgg ccgtcgatcg ggccgacgta
     7441 gttgaggccg aactcctcga agatcgtgcc cggcacgacc atgcccttgg tgtgttcctc
     7501 gaagcggcgc gcgaactcgt acagcggcgt gttcttaagc acgttcttcg cgccctcgcg
     7561 ggccttcgcg tagaacttgc cgctcatgag ccgggccagg tagcggttca gcgcgcccac
     7621 cggcgggctg atcgacatgt cgttgtcgtt gaggatgacc agcatgtcgg ccaccgagac
     7681 gccggcgttg ttcagggcct cgaaggccat gccgccggtc atcgcgccgt cgccgatgat
     7741 ggccacggcc ttgcggtcct cgcccttgag cttggcggcg tgcgccatgc ccagcgcggc
     7801 cgagatcgag gtcgacgagt gcgccgtgcc gaaggcgtcg tactcgctct cgtcgcgctt
     7861 ggggaagccc gagaggccgc cgtactggcg cagcgtgggc atcgcgtcgc ggcggcccgt
     7921 cagcaccttg tgcgggtagg tctggtgccc cacgtcccag accaggcggt cgtgcggcgt
     7981 gttgaacacg tagtgcagcg cgacggtgag ctccacggtg ccgaggttgc tcgacaggtg
     8041 gccgccggtc ttgctgacgc tgtgcagcac gtactggcgc agctcgtcgc agagcgtccg
     8101 cagctcggcc cgggacaggc gccgcaggtc ggctgggctg gcgatcgtcg cgaggaggtt
     8161 ggtggtcatg gtgggcggtg tccgggggcc ggcggcggcg atagccgtca attgtcgcgc
     8221 tcgacgatct tgtcggcgag gcatgcaagg taggcagcct gccctaggcc gctgcgcgcg
     8281 agcgcggcgt gggcctggtc gcggaggtcg agcgcgtagc gctgcgcctg ctcgaggccc
     8341 agcagcgaga cgaacgtggg cttgttctgg tcgacgtcct tgcccgcggt cttgccgagc
     8401 acttcggagg cctgcgtgac gtcgaggaca tcgtccacga cctggaaggc aaggccgacc
     8461 gcggcgccgt agtccgacaa cgccgcgagc gcggccgtgg acgccggacc acaggcggcg
     8521 cccatcatca cgctcgcctg cagcagcacg ccggtcttgc ggcggtgcat gtcgcgcagc
     8581 gccgcctcgt ccaggcaacg gccgacgctc gcgaggtcga tcgcctggcc gccggccatg
     8641 ccgtcgtgcc cggacgcgcg cgccagcatg cgcacgagcc gcgccatcag ctcgggcgcg
     8701 aggccctcgt ccggcgtgag gacgtcgaac gcgagcgcct gcatcgcgtc tcccgccagc
     8761 atcgcctgcg cctcgccgta cttcacgtgc acggtgggct tgccgcggcg caggacgtcg
     8821 ttgtccatgc agggcatgtc gtcgtgcacc agagagtagg cgtggatcag ctccaccgcg
     8881 accgcggctc gcaacgcagc gtcgaggtcg ccgccgaccg cctccgcggc agccatcacc
     8941 agcagcgggc gcagccgctt gccgccgtcg agcacggcgt agcgcatgac gaggccgagc
     9001 tcggcgggcg ccccggccgg aacccagcgc tccagggcgc gctcgacgtc gtcctgccga
     9061 gcgcggaccc accgggcgaa atcatcgatc ttcatgtacg cgtccagggc ttcaattggc
     9121 cctcctccag gactttgact tgttcttcta cggcgtccag cttcgcgcgg cagaactcga
     9181 ggagctgtgc gccccggcga taggcgccca gcaggccatc cagtggcagc tggcccgact
     9241 ccatcgcccg caccagctcg tcgagctccg cctgcgcctg ctcgaagctg gcgggggcgt
     9301 ccgcgggggt aggggcgtcg gtgcgatcca tcaagcggaa atactcggac aaatggggat
     9361 tgtagtcagg gcggccgtcc gcggccccag gggttgcaac gggatgcagc atttcccgaa
     9421 cgcggatgtc atttgtatgg acatccgtgt aaacgacagt cccgaagcgg ttaaaatgtt
     9481 gggttcggcc aacgccgggc acgacgcgtc gcgtgcaccc gcgacgggac ggccggggag
     9541 gcaccccgga tgcccgccac gggactcgcg gcgccagcgg cagccgagac aattcgcata
     9601 gatccacccg cggggccacc gggtgggggt tgacatccgt tcttggagat cctggggatc
     9661 atgtccgacc tgagcatcac gcttgaagct ctggagcgcg cgcgctccca actttccgta
     9721 tccacgtact tcgacgagga cctgttccgg cgcgagcagg aactcatctt ccagcacggg
     9781 ccgcgctacc tcgcgcacga gctggccgtc cccgagatcg gcgactacta cgcgctgccg
     9841 caggaaggcg agggccgcgt gctgatgcgc gggcacgacg gcggcgtgca gctgctgtcc
     9901 aacgtctgcc gccaccgcca ggcggtgatg ctgcgcgggc gcgggcagac cgcgtccaac
     9961 atcgtgtgcc cgctgcaccg ctggacctac gggctcgatg gccggctgat cggcgcgccg
    10021 catttcgacg aggatccctg cctgcacctg aaccgctacc ggacgcgcag ctggaacggc
    10081 ctggtcttcg aggacaacgg gcgcgacgtc gcggccgacc tcgcggcgct cggcccgcgc
    10141 gcggcgctgg atttcggcgg ctacgtcctc gacaaggtgc acgtgcacca gtgcgactac
    10201 aactggaaga cgttcatcga ggtctacctc gaggactacc acgtcggccc gttccacccc
    10261 ggcctcggcg gcttcgtgag ctgcgacgac ctgcagtggg agttcggcac gaactactcg
    10321 gtgcagacgg tgggcgtggc cgaccggctc gctcgcccgg gcagcgccac gtaccgcaag
    10381 tggcacgacg cggtgctggc ctacaacggc ggcgtcgcgc cgcgccacgg cgcgatctgg
    10441 ctcacctact tccccaacat catggtggag tggtatccgc actgcctcgt cgtctccacg
    10501 ctgtacccga agggcccgca gcagacgacc aacgtcgtcg agttctacta ccccgaggag
    10561 atcgccgcct tcgagcgcga gctcgtcgag gcggaacagg cggcctacct cgagacctgc
    10621 gtcgaggacg acgagatcgc cctgcgcatg gacgccgggc gcaaggccct gttcgagcgc
    10681 ggcgacgacg aggccggccc gtaccagagc ccgatggagg acggcatgcg ccacttccac
    10741 gagtggtacc ggcgcgcgat gcagcagccg gcgcgctgaa gcgccgctgc gccggcgagc
    10801 ggccttgcgc cacgccggga caagcgcctg cgcgacgacg tgctgggcga gaatcgcgcg
    10861 atgtccgctt cgctgctgat ggtctgctcc actttcctgt tcgcggcgat gggggtgtgc
    10921 gtcaagctcg cgtcgacccg ctatggcacc ggcgagatcg tgatgtaccg cggcctggtg
    10981 ggcatggtcg gcatcgcgat ggtggcgcgt tcgcgcggcg tcgggctgcg cacccgggtg
    11041 ccgatgatgc atgcctggcg cggcatcgtc ggcgtgacgg cgctcgcgct gtggttccac
    11101 gccatcgccc acctgccgct ggccaccgcg gtcacgctca actacacgtc ctcggtgtgg
    11161 atggcgctgt tcctgatcgg cggcgcggtc gtcgcgggcg cgcagcgggt cgacccgcgg
    11221 ctcgtcgcgc tggtgctcgt gggcttcggc ggcgtcgcgc tggtgctgca tccgacgctc
    11281 gcacgcgacc agctcgcgcc cggcgtgatc gggctcgcct ccggcatgct gtcggcgctg
    11341 gcctacctgc aggtcacgac gctgggtcgc gtcggcgagc cggagctgcg ggtcgtcttc
    11401 tacttctcca tggccggctt cctcgccggg gtcgtcgcga ccgccgtcga gggcgcgcac
    11461 ctgcccacgc tcgccgacgc cgcgcagctc ctgggcatcg gggtgttcgc gacctccgcg
    11521 caggtggcga tgacgcgtgc ctacgcgatc ggccgtccgc tgtccaacgc gagcctgcag
    11581 tacctcggca tcgcgttctc gttcatcttc ggcgtcgtgc tgttccacga cccggtcacg
    11641 ctgtccgcgc tgggcggcat ggcgctgatc gtcgcgacgg ggctcggcgc ctcgctgctg
    11701 cgcgcgcgca cgctcgccgc caccgccccg gacaccctcc ctccgccgca ggactcctga
    11761 catgcccgac accccgaccg ccttcgccct gcccgtgccg ctcgtgagcg cacgcgcgct
    11821 gatggacgcc ctcgccgtcg gcgcgcccgt ggtgctcgtc gactgctcct tcgacctcgc
    11881 ggacaccggc gcgggcgaac gcgccttcga gcggggtcac ctgccgggcg ccgcctacgc
    11941 gcacctggat cgcgacctcg cgggcgtgaa gacgggccgc aacggtcgcc atccgctgcc
    12001 ggcgcgcgtc gactgggcga ccaccctggg ccgcctgggc gcgactcccg cgcgccacgt
    12061 cgtcgcctat gacgcccagg gcggcatgta cgcggcgcgc gcctggtgga tgctgcgctg
    12121 ggcgggccac gcggcggtca gcgtgctgga cggcgggctg caggtctgga cggcggcggg
    12181 cggcgccctc gagtccgggc cgtcgacggt cgccgacgaa ggcgcctatc cgatcgccgc
    12241 gagcctggtg cccgtgatcg aagccgatgc gctgcagaaa tccctgggcc gcgtcacgct
    12301 gctggacgcc cgcgccggcg agcgcttccg cggcgaggtc gagccgctgg acgcccgcgc
    12361 cggccacatc ccgggcgcgc ggtcgcactt cttcaagggc aacctgggcc cggacgggcg
    12421 cttcaggtcg cccgaggcgc tgcgcgcgga cttcgaggcc tacggcgccg cgccgcagga
    12481 ggtcgtgcac cagtgcggct ccggcgtgac ggcctgccac aacctgctcg cgatggagat
    12541 cgcggggcta cccggagcgt cgctctaccc cggctcgtgg agcgaatggt cgtcggaccc
    12601 gtcgaggccg gtcgcggtgg gctgaccgcc cgcggggcgc gggcaccggc cctgccggcg
    12661 agcaggcgcc gggactgcgc aggcgggctt gcaacgaagc acggacggcg cacactggcg
    12721 gtgcgacgtc gtccgcgcct ccgccgcgcg ccaccgcagg agaagctcat gtaccgcagc
    12781 atcctcatcc ccaccgacgg caccgaagtc accgccaagg cggtcgccac cgccgtcgaa
    12841 ctcgcccgcc tgatgggcgc gcgcctgagc acgatcacgg tcaaggagcc gttcccgtac
    12901 agcgcgatct ccgagatgca gccgatcccg ccgcaggagt tctacgacgc ccaggaacgc
    12961 atcgcgctgc ggcgcgtcca cgacgtcgtc gccgccgcgt cggccgccgg cctcgcgtgc
    13021 gaggcgttca cggtcgaggc ggtgcatcca tgggaagcca tcctcgacca cgccaagacg
    13081 cagggctgcg acctcatcgt gatggcctcg catggccggc gcggcgtcgg tgcgctgctg
    13141 ctgggcagcg agaccacgcg cgtgctgacg cattgccagc tgccggtgct ggtggtccgc
    13201 tgatcagggc gccgcgccga agcgggccag ccagtcgacg cagcgccgcc agccatcgcg
    13261 cgcggcggcg gcgtcgtagc tcgggcggta gtccgcgagg aacgcgtggc cggcttcggg
    13321 atacacgacg atctccgacc ggcgcgaggc ctcgctgccc tgcgcgaggc cggccctcat
    13381 gcggtcgacc aggtcaagcg ggatgctcgc gtccttgccg ccgtaaaggc cgagcaccgg
    13441 cgcccgcagc gtccctgcga cctcgaccgg atgcgtcggc ttcagcagcg tcgccggggc
    13501 caggagcttg ccgtaccagg cgacgcccgc cttcacgcgc ggctggtgcg ccacgtacat
    13561 ccacgtgatg cggccgcccc agcagaaccc ggtgaccgcg aggcgcgaga cgtcgccccc
    13621 cctcgtcacc ggcccagtcg acggccgcgt cgaggtcggc catcacctgc gcgtcgggcg
    13681 tgcgctcgac gatctccgac tgcagcttcg cgatgtccct gcagcccttg gcatcgccct
    13741 ggcggacgaa catctccggc gcgacggcga gatacccgag ctttgcgaat cggcgcgcga
    13801 tgtcggcgat gtgctcgtgc acgccgaaga tctcgctgac caccagcagc accggcggcc
    13861 ggcgcacgcc ctgcggccgc gcgcgatagg cgggtatcgc gagatcgccc gacgggatgc
    13921 tcacccagcc ggcctccagc ccgagtgcat cggtgtgcac gactgtggag gcggtcaccg
    13981 gtcgcacggc ggccgcatac ggctccgggg catccggacg cgtcttcccg gtgatgttca
    14041 tgctggcctc ctggacaacg aggacgcggt cgcggcattc ggcgtgcgcg gccgacctcg
    14101 ccaaggatag ccccacgccg tcgcccgccc acggcaggcg gacacctcag ggcacaatcg
    14161 cggccatggc ttcgactacc cctcccgccg tcggcccgct cgccgcgatc gacatgggct
    14221 ccaacagctt ccggctcgag atcggccaag tgcaggaggg ccgttaccgc cggcacctct
    14281 acctgaagga gaccgtgcgg ctcggcgcgg gcctcgacgt cgacggcatg ctcaccgagg
    14341 aggccacgga gcgcggcctc aagtgcctgg ccaccttcgc cgagaccctc aacacgtccg
    14401 cgccgatgca ggtgcgcgcg gtcgcgaccc agacgctgcg ggaggcgcgc aaccgcgaca
    14461 cgttcctcgt gcgcgcggaa gcggcgttgg gtcgcccgat cgagatcatc tccggccgcg
    14521 aggaggcgcg cctgatctac gcgggcgtcg cgttcctgca gccggcgacc aagccgcggc
    14581 tggtcgtcga catcggcggg cggtcgacgg agatgatcct cggcaacggc cgggcgccgc
    14641 gcttggcaga gtccttccag gtcggttgcg tgagcctgtc gatgcgctac ttcggcgatg
    14701 ggcgttacac gcgggcggct ttccgcgcgg cccaggtcgc ggcgggcgcg gagctcgaag
    14761 aaggcctggt gccgttcgca cgacggcact ggcgcgaggc gctggggtcg tcgggtacgg
    14821 cgggcgcggt gtcggacctg ctgcgcgcca atggcaagac cgacggccgc atcacgcccg
    14881 agggcctgcg ttggctcatc gaacgctgca tcgagtgcgg ccacatcgac aagctggaca
    14941 tcccgggact gaagcccgag cgccgcgcgg tgatcgctgg cggcctggca atcctctaca
    15001 cgttggcggc gcaattcggc atcgaggagc tgctgcccgc caagggtgcg cttcggcagg
    15061 gcgtgatcgt cgatctcagc gagcgcctgg ccgccagtca cgaagcggtc ggccacgacc
    15121 tgcgcgactc gaccatcaag gagctccagc atcgcttcca cgtcgacgtc gcccaggccg
    15181 gacgcgtggg cgaggcagcc cggcggctgt tcgacgatgt ctgccaggac gcggacgccg
    15241 agtgccagcg cgagctacgc tgggccgcgg cgcttcacga ggccggcctg atggtgtcgc
    15301 accacgacca ccatcggcac agcgcctaca tcattgcgca cgtggatgcg ccggggttct
    15361 cgcagagcca acagcggcgt ctcgcggaac tggtgcttgg ccagcgcggc ggactgcgaa
    15421 agatcgaggc ggcgctcgcc cgcccgctga cgccgtggca ggtactctgt ctgcggctcg
    15481 ccgtgatcga gtgccatgcg cgcgggcaag tcgatgcgcg cggcctcaag cttcgccgcg
    15541 tcggcaccga ggcgcatctg agcttcactc cggaatgggc cgaagcccat ccacgcaccg
    15601 cctacctcct gcgcgaggaa gccgcattgt gggcacgaca gggcgtgctg ctgctcaagc
    15661 tcgattgagc gatcgcggcg caaggccgcg atgatgcccg ccaaccgaca cgcaccaccc
    15721 acgccagacg acaagcgggc cgccgtgcga tagatagccc ttcgtgtcgc ccaaatcttc
    15781 cggcgcgagg atggcgcctg gaagccgggg caccgacacg ccgacacgtc cgacgacacg
    15841 cagcccgccg tgagatggcg cggccagtct cccgagggca gttcggagaa ttacagtcga
    15901 ttcgatgccg caaagcaaaa cgcccgagct gaaggtttca gctcgggcgg ttgcgggata
    15961 ttagcctgac aatgtcctac tttcacacgg gaatccgcac tatcatcggc gcagaggcgt
    16021 ttcactgtcc tgttcgggat gggaagga
//
LOCUS       DASVLN010000362         3860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_180446, whole genome shotgun sequence.
ACCESSION   DASVLN010000362 DASVLN010000000
VERSION     DASVLN010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180446"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..881
                     /locus_tag="VF453_08935"
     CDS             189..881
                     /locus_tag="VF453_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7687818.1"
                     /translation="MTPIVLVPGLLATADLFAPQLDALWPHGPVTFASTLAGTSLPEM
                     AAALLASAPPRFALAGWSLGGFVALEVMRQAPARVLRLALLDTSARPDTPEESAQRRE
                     WVARARQGEFEAMQPRAVRMLLHPDRRTDHALVDRLMRMGRTVGVDGFARQTAAVVGR
                     PDARPQLRGIGVPTLVLVGERDELTPPEQARELAEGIPGAHLVVVPGAAHATTLEQPE
                     AVSRALVDWIGG"
     gene            complement(878..1882)
                     /locus_tag="VF453_08940"
     CDS             complement(878..1882)
                     /locus_tag="VF453_08940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX7687819.1"
                     /translation="MSRETVLVARRVFPDIVDRLRAHFDVREHAGDAPLPPPELAAAL
                     RGCAGAFIAGSERIDAALLDACPGLKAVCSMAVGFNNIDVAACTARGVVVTNAPGVLT
                     ETTADFGFALMMATARRISEAERFLREGRWSHWGVDLFAGADIHGATMGVLGMGRIGQ
                     AIARRGHGGFGMKIIYHNRSRLAVADEAALGATWLPLDELLAQADHLVIVTPYTPETH
                     HLVGAAQLARMKPTATLTNVARGGVVDDAALAHALGAGTIAAAGLDVFEGEPALHPAL
                     LALRNVVLTPHIASASVPTRRAMAGLAVDNLIAALGAGPDAGRPPTALNPQVLEARRR
                     "
     gene            complement(1941..2660)
                     /locus_tag="VF453_08945"
     CDS             complement(1941..2660)
                     /locus_tag="VF453_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX7687820.1"
                     /translation="MSDPRSPTPPDDAARRAVDAAITSRRSVRAFLPTPVPAETVREI
                     LEVASRAPSGTNTQPWRVHVLMGQTRARLSERILAAYDDPGERARHVEAYDYYPREWV
                     SPYIDRRRKVGWDLYGLLGIGKADKAAMHAQHGRNYAFFGAPVGFIFTIDRVMAQGSW
                     LDYGMFLENIMVAARGRGLDTCPQAAFNQFHRVIADVLGLGEQEAVVCGMALGHADPD
                     AVANRLTTERARVDEFARFHE"
     gene            complement(2657..3136)
                     /locus_tag="VF453_08950"
     CDS             complement(2657..3136)
                     /locus_tag="VF453_08950"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7687821.1"
                     /translation="MTDAPPARRAPEPRSAFAAFSPITTRWMDNDVYGHVNNVVYYSF
                     FDTAVNGLLIAAGALDIHAGAVIGLVVETQCNYFAPIAFPQAVSAGVRVARLGGSSVR
                     YEIGLFADDAPQSAASGHFVHVYVDRATRRPVPLPGPLAAVLRTWMLPNPAPTASPT"
     gene            complement(3189..>3860)
                     /locus_tag="VF453_08955"
     CDS             complement(3189..>3860)
                     /locus_tag="VF453_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7687822.1"
                     /translation="ARTALAPGGADPAAAGIAASLGGGTHHASAAKGSGFCVFNDVAV
                     AARLMQAEWHRHRRALLPVVVVDLDVHQGNGTAAIFADDDTVFTFSMHGERNFPFRKS
                     PGDLDVGLPDGSGDDDYLAALAPALGEVWRRTAARPPGLAFYVAGADAHEGDRLGRLR
                     LTHAGMAARDELVLGALRERGIPTVVLMAGGYGRDLEATVDVHLRTLRIAERLQGTWP
                     RAVPA"
BASE COUNT          474 a         1409 c         1493 g          484 t
ORIGIN      
        1 cgcgccggtg ccgatggcct cggcggcggc ggacacggcg gcctcggccg cgtcagcgcc
       61 ctgagcgcga actcaccctt cccgcccgag gccggccgcc gcgccggcct ttttcatgcg
      121 cgccggccgc tcgtccacga ggcgcgccga ttgctccgac ggcccgcccc cgcgacccgg
      181 agccgtccat gaccccgatc gtcctcgtcc ccggcctgct ggcgaccgcc gacctgttcg
      241 cgccccagct cgacgccctg tggccgcatg gccccgtcac gttcgcctcg accctcgcgg
      301 gcaccagcct gccggagatg gccgccgcgc tgctcgccag cgcgccgccc cgcttcgcgc
      361 tggcaggctg gtcgctgggc ggcttcgtcg cgctcgaggt gatgcgccag gcccccgcgc
      421 gcgtgctgcg gctggcgctg ctcgacacct ccgcccgccc cgacacgccc gaggaatcgg
      481 cgcagcgccg cgagtgggtc gcgcgcgcgc gccagggcga gttcgaggcg atgcagccgc
      541 gcgccgtgcg gatgctgctg catcccgacc gccgcaccga ccacgcgctc gtcgaccgtc
      601 tcatgcgcat gggacgcacc gtgggcgtgg atggcttcgc gcgccagacg gccgcggtcg
      661 tcggccgccc ggacgcgcgc ccgcagctgc gcggaatcgg cgtgccgacg ctggtgctgg
      721 tcggcgagcg cgacgagctc accccgcccg agcaggcgcg cgagctcgcc gagggcatcc
      781 ccggggcgca cctcgtcgtc gtgccgggcg cggcccacgc gacgacgctg gagcagcccg
      841 aggcggtctc ccgcgccctg gtcgactgga tcggcggtta gcgccgccgc gcttccagca
      901 cttgcggatt gagcgcggtc ggcggccgcc ccgcgtcggg gcccgcgccg agcgcggcga
      961 tcaggttgtc gacggcgaga ccggccattg cacgccgcgt cggcacgctg gcgctcgcga
     1021 tgtgcggggt cagcacgacg ttgcgcagcg cgagcagcgc cggatgcagc gccggctcgc
     1081 cctcgaagac gtccagcccc gccgccgcga tcgtgccggc gcccagcgcg tgcgcgagcg
     1141 ccgcgtcgtc gaccacgccg ccgcgcgcga cgttggtcag cgttgccgtc ggcttcatgc
     1201 gggcgagctg ggccgcgccg acgagatggt gcgtctcggg cgtgtacggc gtgacgatca
     1261 cgaggtggtc ggcctgcgcc agcagctcgt ccagcggcag ccaggtcgcc ccgagcgcgg
     1321 cctcgtccgc gaccgcgagc cggctgcggt tgtggtagat gatcttcatg ccgaagccgc
     1381 cgtgcccgcg gcgcgcgatc gcctggccga tgcggcccat gcccagcacg cccatcgtcg
     1441 cgccgtggat gtcggcgccg gcgaacaggt cgacgcccca gtgcgaccag cgcccctcgc
     1501 gcaggaagcg ctcggcctcc gagatgcggc gcgccgtggc catcatgagc gcgaagccga
     1561 agtcggccgt ggtctcggtg agcacgccgg gcgcgttggt gacgacgacg ccgcgcgccg
     1621 tgcaggccgc cacgtcgatg ttgttgaagc cgaccgccat cgagcagacg gccttcaggc
     1681 ccgggcaggc gtcgagcagc gcagcgtcga tccgctcgct gccggcgatg aacgcgccgg
     1741 cgcagccccg caaggcggcg gccagctcgg gcggcggcag cggcgcgtcg cccgcgtgct
     1801 cgcgcacgtc gaagtgagcg cgcaggcggt cgacgatgtc tggaaacacg cggcgcgcga
     1861 cgaggacggt ctcgcggctc acgaggcgac cgtccttcgg accgcggtgc gagccccctt
     1921 cggaacggcc gggcgggggc tcattcgtgg aacctcgcga actcgtcgac ccgcgcgcgc
     1981 tcggtcgtca ggcggttggc cacggcgtcg ggatcggcgt ggcccagcgc catgccgcag
     2041 acgacggcct cctgctcgcc caggcccagg acgtccgcga tcacccggtg gaactggttg
     2101 aaggccgcct gcgggcaggt gtcgaggccg cgcccgcgcg cggcgaccat gatgttctcg
     2161 aggaacatgc cgtagtcgag ccagctgccc tgggccatca cgcggtcgat cgtgaagatg
     2221 aagcccaccg gcgcgccgaa gaacgcgtag ttgcggccgt gctgcgcgtg catcgccgcc
     2281 ttgtcggcct tgccgatgcc cagcaggccg tacagatccc agccgacctt gcgccggcgg
     2341 tcgatgtagg gcgagaccca ttcgcgcggg tagtagtcgt acgcctcgac gtggcgggcg
     2401 cgctccccgg ggtcgtcgta ggccgcgagg atgcgctcgg acaggcgcgc ccgcgtctgg
     2461 cccatcagga cgtgcacccg ccagggctgc gtgttcgtgc ccgagggcgc gcgcgaggcg
     2521 acctccagga tctcgcgcac cgtctcggcg ggcacgggcg tgggcaggaa ggcgcgcacg
     2581 gagcggcgcg aggtgatggc cgcgtcgacg gcacggcggg cggcgtcgtc gggtggagtc
     2641 ggggatcggg gatcgctcat gtcggtgatg cggtcggggc ggggttggga agcatccacg
     2701 tgcgcagcac cgccgccagc ggcccgggca acggcacggg gcgccgcgtc gcgcggtcga
     2761 cgtagacgtg gacgaagtgg ccggacgcgg cggactgcgg cgcgtcgtcg gcgaacaggc
     2821 cgatctcgta gcgcacgctg gagccgccca ggcgcgcgac gcgcacgccg gcgctcaccg
     2881 cctgcgggaa ggcgatcggc gcgaagtagt tgcactgcgt ctcgacgacc aggccgatca
     2941 cggcgcccgc gtggatgtcg agcgccccgg cggcgatcag caggccgttg accgcggtgt
     3001 cgaagaagct gtagtagacg acgttgttga cgtggccgta gacgtcgttg tccatccagc
     3061 gcgtcgtgat cgggctgaag gccgcgaagg cggagcgggg ctcgggcgcg cggcgggcgg
     3121 gcggggcgtc ggtcatcggg gccggcgaag gacggtggcg gagcgggcca ttgtgggcca
     3181 cgcgcgcgtc aggccggcac ggcgcgcggc catgtcccct gcaggcgctc ggcgatgcgc
     3241 agcgtgcgca ggtggacgtc gacggtggcc tcgaggtcgc gtccgtagcc gccggccatc
     3301 agcacgacgg tcgggatgcc gcgctcgcga agcgcgccca gcaccagctc gtcgcgggcg
     3361 gccatgccgg catgggtcag ccggaggcgg ccgaggcggt cgccctcgtg cgcgtcggcg
     3421 cctgccacgt agaacgcgag ccccggcggc cgcgcggcgg tgcggcgcca gacttcgccc
     3481 aaggcgggcg cgagcgcggc gaggtagtcg tcgtcgccgc tgccgtcggg caggcccacg
     3541 tcgaggtcgc caggcgactt tcggaacggg aagttgcgct cgccgtgcat cgagaacgtg
     3601 aacacggtgt cgtcgtccgc gaagatcgcc gccgtgccgt tgccctggtg gacgtcgagg
     3661 tcgaccacca cgaccggcag caacgcgcgg cggtggcgat gccactcggc ctgcatcagc
     3721 cgcgccgcga cggcgacgtc gttgaagacg cagaagcccg agcccttggc ggcgctcgcg
     3781 tggtgggtgc cgccgcccag gctcgcggcg atccccgccg cggccggatc ggcgccgccg
     3841 ggcgcgaggg cggtgcgcgc
//
LOCUS       DASVLN010000363         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_72359, whole genome shotgun sequence.
ACCESSION   DASVLN010000363 DASVLN010000000
VERSION     DASVLN010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72359"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1682
                     /locus_tag="VF453_08960"
     CDS             <1..1682
                     /locus_tag="VF453_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7687823.1"
                     /translation="RDPSDTPVPPPPPAPAPPRDLPRPVAVRRAHYSLWLVWLVPLVA
                     VIIGIGLGVRSILNRGPVITIYFHNADGVEANKTHIKYKDVDVGIVRRVKLTPDHKQV
                     EVTAEMRGNEGVENMLVTDTRFWVVKPRIGAGGVTGLGTLLSGAYIGMDAGRNATERS
                     EFQGLDTQPPVTTDVPGRVFVLQSDELGSLDIGSPLYLRRVPVGQVMAYEMTPDGRHV
                     RFTVFVNAPYDRFVNQHSRFWHASGVDVALDAQGLHVQTQSVVSLLAGGIAFQDPPDV
                     DPVGQPEAAERTQFTLFTQQADALRTDDGPGYDYRLKFASSVRGLAVGAPVEYRGLPI
                     GEVRRIAVDGAAGSDNPEPMIAVDVRVWPQRLPTIDGDRTTDTAAQQRARLDPMVRRG
                     FRAQLRSGSLLTGQLYVGLDFFKGAPPAAIDWKASPPVMPTMPGSLDSLQDSLTSLAK
                     KLDAIPYDQLASDLHRDLSDLDTSLRHVDQLVQHLDGDIAPEAKRTLSDARAAVDDLR
                     RTLGTVDQAVGPKATQTLRDVSKAADSLRGLADYLERHPESLLKGKPEDPK"
     gene            1790..>2106
                     /locus_tag="VF453_08965"
     CDS             1790..>2106
                     /locus_tag="VF453_08965"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEX7687824.1"
                     /translation="MVAVVAGCSTPPDHYHTLRPVLAAGAAHDARGTLAVGPVTVPDA
                     LDRNEWVVRSGETGAAVYDHQLWTQDLSAEVAQALADDLNEAALPGGLWADAGPTGSG
                     SGA"
BASE COUNT          301 a          795 c          742 g          268 t
ORIGIN      
        1 accgcgaccc gtccgacacg cccgtcccgc cgcctccgcc ggcgccggcg ccaccgcgcg
       61 acctgccgcg gcccgtcgcc gtgcggcggg cgcactactc gctgtggctg gtgtggctcg
      121 tgccgctggt ggcggtgatc atcggcatcg gcctgggcgt gcggtcgatc ctgaaccgcg
      181 ggccggtcat cacgatctac ttccacaacg ccgacggcgt cgaggccaac aagacccaca
      241 tcaagtacaa ggacgtggac gtcggcatcg tgcgccgcgt caagctcacg cccgaccaca
      301 agcaggtcga ggtgaccgcg gagatgcgcg gcaacgaggg cgtcgagaac atgctcgtca
      361 ccgacacccg cttctgggtc gtgaagccgc gcatcggcgc cggcggcgtc acggggctgg
      421 gcacgctgct gtcgggcgcc tacatcggca tggacgccgg ccgcaacgcc accgagcgca
      481 gcgagttcca gggcctggac acgcagccgc ccgtcaccac cgacgtcccc ggccgcgtct
      541 tcgtgctgca gtccgacgag ctcggttcgc tcgacatcgg ctcgccgctg tacctgcgcc
      601 gcgtgccggt gggccaggtg atggcctacg agatgacccc cgacggccgc cacgtgcgct
      661 tcaccgtgtt cgtgaacgcg ccgtacgacc gcttcgtcaa ccagcacagc cgcttctggc
      721 acgcgagcgg ggtcgacgtc gccctcgacg cccagggcct gcacgtgcag acgcagtcgg
      781 tcgtgagcct gctggcgggc ggcatcgcgt tccaggatcc gcccgacgtc gacccggtcg
      841 gccagcccga ggcggccgag cgcacgcagt tcacgctgtt cacgcagcag gccgacgcgc
      901 tgcgcaccga cgacggcccg ggctacgact accggctgaa gttcgcgtcg tccgtgcgcg
      961 ggctcgcggt gggcgcgccg gtcgaatacc gcggcctgcc gatcggcgag gtgcgccgca
     1021 tcgccgtcga cggggcggcc ggcagcgaca accccgagcc gatgatcgcg gtggacgtgc
     1081 gcgtctggcc gcagcgcctg cccacgatcg acggcgaccg cacgaccgac accgcggcgc
     1141 agcagcgcgc gcggctcgac ccgatggtgc gccgcggctt ccgcgcccag ctgcgcagcg
     1201 gcagcctgct caccggccag ctctatgtcg gcctggactt cttcaagggc gcgccacccg
     1261 cggccatcga ctggaaggcc agcccgccgg tcatgccgac gatgccgggc agcctcgaca
     1321 gcctgcagga ttcgctgacc tcgctcgcga agaagctcga cgcgatcccg tacgaccagc
     1381 tggcctccga cctgcaccgc gacctgtccg acctcgacac gagcctccgg cacgtcgacc
     1441 agctcgtgca gcacctcgac ggcgacatcg cgcccgaggc gaagaggacg ctctccgatg
     1501 cccgcgccgc ggtcgacgac ctgcgccgca cgctcggcac cgtcgaccag gcggtcgggc
     1561 cgaaggcgac gcagacgctc agggacgtct ccaaggcggc cgactcgctg cgcggcctcg
     1621 ccgactatct cgaacgacat cccgaatcgt tgctcaaggg caagcccgag gatccgaaat
     1681 gacatccccc ccgcagcgcc tgcggcgcct ccccccgagg gggcgcaccc agcggcccgg
     1741 caaagccggt tccgcggctg ccgcttgtag gcggtggctg gcgtctctcg tggtggcggt
     1801 tgtggcgggg tgttcgacgc cgccggacca ctatcacacg ctgcggccgg tgctggcggc
     1861 cggggcggcg catgacgcgc gggggacgtt ggcggtcggg ccggtcacgg tgcccgacgc
     1921 gctggaccgc aacgagtggg tggtgcgcag cggggagacc ggcgccgcgg tctacgacca
     1981 tcagctgtgg acgcaggacc tgtcggccga ggtcgcgcaa gccctggccg acgacctgaa
     2041 cgaggccgcg ctgcccggcg gcctgtgggc cgacgccggg ccgaccggct cgggcagcgg
     2101 cgccga
//
LOCUS       DASVLN010000364         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_340763, whole genome shotgun sequence.
ACCESSION   DASVLN010000364 DASVLN010000000
VERSION     DASVLN010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340763"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..566)
                     /locus_tag="VF453_08970"
     CDS             complement(96..566)
                     /locus_tag="VF453_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7687825.1"
                     /translation="MSDDTSRLALVFRIAARLCALPLAQVVEIMRPLPVRQLDGTPGF
                     VLGAAMVRGLPTPVVSGARLLGGEDAPAGGGRWIGVVAGSRRVMLEVDEVGGIRRIPA
                     GDGAAAQPLLEGVAADRIATLGRLDGELLSTLQAARVVPELAWSAIDAALGAAA"
     gene            complement(778..>1723)
                     /locus_tag="VF453_08975"
     CDS             complement(778..>1723)
                     /locus_tag="VF453_08975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7687826.1"
                     /translation="AATATTAITASRPAPAAASAPRSAAEILRDAPDAAWRRPDPNDL
                     LVMTLPSGPVVIELAERFAPLHAANIRKLVAQRYFDGLALVRVQDNFVAQWADPEAEA
                     EQVAASAPAGHPRPLGEASATLPPEFSIPLADARIARLPDRDVWAPVTGFVDGFPVAA
                     DPKTRRAWIAHCYGIVGAGRGDTLDSGNGAELYAVIGQSPRALDLNIAPVGRVLQGME
                     RLASLPRGGAEMGFYQRREQDVPILRVELVAAMPPEARPDVRVLRTDTPLWAELVDAR
                     RRHRDAWYVHSPGAIDLCNVTVPVRIAAPPGAAASTPP"
BASE COUNT          226 a          645 c          649 g          203 t
ORIGIN      
        1 gaccgcgtcg cggaatcgcc ggcactccgc cggcgacgcc gcgacggctc gcgcgtcgca
       61 gtgcgacccg cgggccggca ggaggcggcg aaggttcacg cggcagcccc caatgcggcg
      121 tcgatggccg accaggccag ctcggggacg acgcgcgcgg cctgcagcgt gctcagcagc
      181 tcgccgtcga ggcggcccag cgtcgcgatg cgatcggctg cgacgccttc gagcaggggt
      241 tgcgcagccg cgccgtcgcc ggcggggatc cgccggatgc cgccgacctc gtcgacttcc
      301 agcatgacgc gccgcgaccc ggcgacgacg ccgatccagc gccctccgcc ggcaggcgca
      361 tcctctccgc ccagcaggcg cgcgcccgag acgaccggcg tcggcagccc ccgcaccatg
      421 gccgcaccga gcacgaagcc gggcgtgccg tcgagctgcc gcaccggcag cggacgcatg
      481 atctcgacca cctgcgcgag cggcaatgcg cacagcctgg cggcgatgcg aaacaccagt
      541 gcgaggcggg acgtgtcgtc gctcacgttt tctttgctcc cggcgggcca aggagcgccc
      601 cgccacagaa cccatccggt ctcgcggcgg ccacgccccg gcggccgcga atccccggat
      661 ctcccttacg actcctgccg tccagattag cgcgccgcgc agggttcgcg caagccgcac
      721 atgtgccgat accccatgcg gcatccggct ttacatgcgc gagccttgcc gaagcgctca
      781 gggtggcgta gacgccgccg ccccgggcgg cgccgcgatg cgcaccggaa cggtcacgtt
      841 gcacaggtcg atcgcgcccg gcgagtgcac gtaccaggcg tcgcggtggc ggcggcgcgc
      901 atcgacgagc tcggcccaca gcggggtatc ggtgcgcagc acgcgcacgt ccgggcgcgc
      961 ctcgggcggc atcgcggcga cgagctcgac gcgcaggatg ggcacgtcct gctcgcgtcg
     1021 ttggtagaag cccatctcgg cgccgccgcg cggcagggag gccaggcgct ccatgccctg
     1081 cagcacgcgg ccgaccggcg cgatgttcag gtccagcgcg cgcggcgact ggccgatcac
     1141 ggcgtagagc tcggcgccgt tgccgctgtc gagcgtgtcg ccgcggccgg cgccgacgat
     1201 gccgtagcag tgcgcgatcc aggcgcggcg cgtcttcggg tcggcggcca ccgggaagcc
     1261 gtcgacgaag ccggtgaccg gcgcccagac gtcgcgatcc ggcaggcggg cgatcctcgc
     1321 gtcggccagc gggatcgaga actcgggcgg cagcgtggcg ctcgcctcgc ccagcgggcg
     1381 cgggtggccg gccggcgccg aggccgccac ctgctccgcc tccgcctcgg gatcggccca
     1441 ttgcgcgacg aagttgtcct gcacgcgcac cagcgccagg ccgtcgaagt agcgctgcgc
     1501 gacgagcttg cggatgttgg ccgcatgcag gggcgcgaag cgctccgcca gctcgatgac
     1561 gaccggcccc gacggcagcg tcatcacgag caggtcgttc gggtcgggac ggcgccaggc
     1621 ggcgtcgggc gcatcgcgca ggatctcggc ggcgctgcgc ggcgcgctcg cggcggcggg
     1681 tgcgggccgg ctcgcggtga ttgcggtggt tgcggtggcc gcg
//
LOCUS       DASVLN010000365         5840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_503763, whole genome shotgun sequence.
ACCESSION   DASVLN010000365 DASVLN010000000
VERSION     DASVLN010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503763"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VF453_08980"
     CDS             complement(<1..757)
                     /locus_tag="VF453_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7687827.1"
                     /translation="MDKRYGAVKVLEGATLRLRAGEVHTLMGQNGAGKSTLIKVLTGV
                     VPADGGTLALAGAAIAPDSPIAAQRLGISTVYQEVNLCPNLTVAENIFAGRYPRRGWS
                     RLKAIDGPRMREETRALLARLHLDIDPSQLLGRLPVAMQQLVAIARALSVQAKVLILD
                     EPTSSLDEDEVQHLFGILRQLREAGLAILFVTHFLDQVYAIADRITVLRNGRFVGEWT
                     PAQLPAPALVAAMVGRDVALASASADGVAAQTHA"
     gene            complement(920..1879)
                     /locus_tag="VF453_08985"
     CDS             complement(920..1879)
                     /locus_tag="VF453_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7687828.1"
                     /translation="MTIQRRTVLASTLLAAAYGLPTLAFGQKKIVLGFSQVGAESGWR
                     TANTESIKSAAKEAGIELKFADAQQKQENQIKAIRSFIAQKVDVIAFSPVVETGWDPV
                     LHEAKAAHIPVILTDRSVAADPSLYVTFMGSDFVEEGRRAANWLEKFEQGKPGPINIV
                     ELQGTVGSAPAIDRKKGFEEVISKDSRMKIIRSQTGDFTRAKGKEVMEAFLKAEGKNI
                     NVLYAHNDDMALGAIQAIEEAGMKPGTDITIISIDGTRAAFQAMIDGKQNVVVECNPL
                     LGPQLMSATKDLVAGKPLPKRIVTTESVYPREVAAAEFPKRQY"
     gene            2139..2579
                     /locus_tag="VF453_08990"
     CDS             2139..2579
                     /locus_tag="VF453_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7687829.1"
                     /translation="MASVNAYDIRTDPEAMDIAAIHDFLSTAYWSPGVPREVVARAVA
                     NSVCVGAFDAAGGQVGFARMVSDRATFAYLSDVYVLQAHRGHGLARRMVQALLAHPEL
                     QGLRRVMLATRDAHGLYAQLGWKPLAAPERFMEIHQPDVYGRAG"
     gene            2858..3880
                     /locus_tag="VF453_08995"
     CDS             2858..3880
                     /locus_tag="VF453_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX7687830.1"
                     /translation="MTLAFVLLVAAAAVIYLACEFFVNGVEWVGHRFGVARTAVGTVL
                     AAFGTALPESVVTLVAVVFGRTPGQRDIGVGAALGGPLALSTLAYAVVGLVFLVDRRH
                     RGGGIVREFSTRALAHDQGWFLVVFVSKVALGLVAFAWKPWLGTLFVLAYALYVWREL
                     RAEDPAGGCEGDDAPDDADFERLKLRPHAANPSTGWAVLQTTGALVVIFLASQLFVRQ
                     LGVVGPGLGMSPQLVALLLSPIATELPEILNAVIWVRQGKTALALGNISGAMMIQATI
                     PSALGIWFTPWLLDRPLLLAAGVTMAAIAVTLVLLRRHALTAPRLVALAGLYGVFAAG
                     VAWMWR"
     gene            complement(3908..4855)
                     /locus_tag="VF453_09000"
     CDS             complement(3908..4855)
                     /locus_tag="VF453_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEX7687831.1"
                     /translation="MCLVGLAIDENRRFPLVIAANRDEFFDRPAARLAWWQPAEGGPQ
                     ILAGRDLSAGGTWLGLSAAGRLALVTNVRRIVEPDPHAPSRGGIVPRWLRTELRPDQF
                     WMQTSLSGYAPFNLIAADFRRGECFWASNEQAIPRRIERGIFGLSNAALDTPWPKVEA
                     LKARLGEALDAADSVDALSSMLFAALSDRGVPPDDRLPNTGIGRERERVLAPAFVRTP
                     DGRYGTRCSTVIVTEKIKRQLVTHVFERTFTAGPGVALLRRAVLKDWPPRYADRAASA
                     VEISAVAETELEPGAAVAPMPPLPAKRTRVRSLLKPASK"
     gene            complement(4843..5292)
                     /locus_tag="VF453_09005"
     CDS             complement(4843..5292)
                     /locus_tag="VF453_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687832.1"
                     /translation="MTDITTAPGVPGATLLACVYYKVAAADLRSAISTVREFQRTLPA
                     LAGAGGAQVLLRCALPRDDASSTRSNASESPTEHASPAAPPSGAAVAPDVEHTLMETY
                     TLPLPADPAAASAATAAFLAALDRAAAPLAALVRGARHTELFRPCAS"
     gene            complement(5339..>5840)
                     /locus_tag="VF453_09010"
     CDS             complement(5339..>5840)
                     /locus_tag="VF453_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEX7687833.1"
                     /translation="GGRARVLWAAPADASPPWPAATDATTWNWLEVQAGVPRVVARTA
                     EAFVPQMVNLELVGGVNFKKGCYPGQEIVARSQYRGTVKRRMVLVEGEAAPEAGTEVF
                     SPAEPDQPAGQVVLSASLAGGRHAALVEVKRALVDGGEFHAGAADGPRLAVRELPYAL
                     PAIDAE"
BASE COUNT          722 a         2028 c         2190 g          900 t
ORIGIN      
        1 cggcgtgcgt ctgggcggcg accccgtcgg ccgaggccga cgcgagcgcg acgtcgcgac
       61 cgaccatcgc cgcgacgagc gcgggcgcgg gcagctgcgc gggcgtccat tcgcccacga
      121 agcggccgtt gcgcagcacg gtgatgcggt ccgcgatcgc gtagacctgg tcgaggaaat
      181 gggtgacgaa caggatcgcg agcccggcct cgcgcagctg gcgcaagatg ccgaacaggt
      241 gctgcacctc gtcctcgtcg aggctggagg tcggctcgtc gaggatcagc accttggcct
      301 gcacgctgag cgcgcgcgcg atcgcgacga gctgctgcat cgccaccggc agccgcccga
      361 gcagctgcga cgggtcgatg tccaggtgca ggcgggcgag cagggcgcgc gtctcctcgc
      421 gcatgcgcgg gccgtcgatc gccttgagcc ggctccagcc gcggcgcggg tagcggccgg
      481 cgaagatgtt ctccgccacc gtgaggttgg ggcagaggtt gacctcctgg tacaccgtgc
      541 tgatgcccag gcgctgggcg gcgatcggcg agtccggcgc gatggccgca cccgccagcg
      601 cgagcgtgcc gccgtcggcc ggcacgacgc cggtgagcac cttgatcagc gtggacttgc
      661 ccgcgccgtt ctgccccatc agcgtgtgca cctcgccggc gcgcaggcgc agcgtcgcgc
      721 cctcgagcac cttcaccgcg ccgtagcgct tgtcgatggc gtccgccgcg aggacgatgc
      781 caccgtccgg gcgggccgca gccccgcccc cctctcgggc gtccggccgg ggatctgtca
      841 cgaccacccc ccgcggcagg tcatccactc ctcgccgacc ctgcggtcat gcctcgtgac
      901 ggacgccatc gacgcgcgct cagtactgac gcttcgggaa ttccgcggcg gccacctcgc
      961 gcggatacac gctctcggtg gtgacgatgc gcttgggcag cggcttgccg gcgacgaggt
     1021 ccttcgtcgc ggacatcagc tgcgggccca gcagcgggtt gcactcgacg acgacgttct
     1081 gcttgccgtc gatcatcgcc tggaaggccg cgcgcgtgcc gtcgatcgag atgatcgtga
     1141 tgtcggtgcc gggcttcatg ccggcctcct cgatcgcctg gatcgcgccg agcgccatgt
     1201 cgtcgttgtg cgcgtagagc acgttgatgt tcttgccctc tgccttcagg aaggcctcca
     1261 tgacctcctt gcccttggcg cgggtgaagt cgccggtctg cgagcggatg atcttcatcc
     1321 gcgagtcctt ggagatgacc tcctcgaagc ccttcttgcg gtcgatcgcg ggcgccgagc
     1381 cgacggtgcc ctgcagctcg acgatgttga tcgggccggg cttgccctgc tcgaacttct
     1441 cgagccagtt ggcggcgcgg cggccttcct cgacgaagtc ggaacccatg aaggtcacgt
     1501 acaggctggg atcggccgcc accgagcggt cggtgaggat gacggggatg tgcgcggcct
     1561 tggcctcgtg cagcacggga tcccagcccg tctcgaccac cggcgagaac gcgatcacgt
     1621 cgaccttctg cgcgatgaag gagcggatcg ccttgatctg gttctcctgc ttctgctgcg
     1681 cgtcggcgaa cttcagctcg atgccggcct ccttggccgc ggacttgatc gactcggtgt
     1741 tggcggtgcg ccagccgctc tcggcgccga cctggctgaa gcccagcacg atcttcttct
     1801 gcccgaaggc gagggtgggg agcccgtagg cggcggccag cagggtcgat gcgaggaccg
     1861 tgcgtcgttg gatggtcatg gtatgtcgtc tcctgtcgtt cgttcgttcg ccgccgtcga
     1921 gagagcgggc ccgtggccgt ccggcgtggt cgcatgcggc cgccgggccg gttgggcatg
     1981 catgctagaa gagtggatcg atagcaggca aatcgtttta tcgcggccag ccatatgcga
     2041 tttcgtatcg atggaaaccc tggcgagcgg cgtcggcatg tcccgttcct gcgcttcgct
     2101 tcgggcaagg ctcggatggt cggccgctcg gcgccagaat ggccagtgtg aacgcctacg
     2161 acatccgcac cgaccccgag gcgatggaca tcgccgccat ccacgacttc ctgtccaccg
     2221 cctactggtc gcccggcgtc ccgcgggagg tggtcgcgcg cgccgtcgcg aattccgtgt
     2281 gcgtgggcgc cttcgacgcg gcgggcggcc aggtcggctt cgcgcgcatg gtcagtgacc
     2341 gcgcgacctt cgcgtacctg tccgacgtct acgtgctgca ggcgcaccgc ggccacggcc
     2401 tcgcgcgccg catggtgcag gcgctgctcg cgcatcccga gttgcagggc ctgcgccgcg
     2461 tgatgcttgc cacgcgcgac gcgcacgggc tctacgcgca gctcggctgg aagccgctcg
     2521 cggcgccgga gcgcttcatg gagatccacc agcccgacgt ctacggccgc gccggttgag
     2581 cgcccggcct gtgctacagt gcgccccgtc cttgggggag tagccgcctt cacgtccgtg
     2641 gagagcgtcg gtcaacatgc ttggcgcgtc ctgcgccatg gccggcgcgg cctggagttt
     2701 ccaggcttgg caagaccttt ggcacgcgcg ttccgacccg gggccggggg agcgtcgcgt
     2761 cgccatcggt gcgttgtccc ggcctcgtga agatccccat gagaatcctg cctgccatcg
     2821 atccaccgac gccgcgcacg tgcggccgga gaacgcgatg acgctcgcgt tcgtcctgct
     2881 ggtcgccgcg gcggccgtga tctacctcgc gtgcgagttc ttcgtcaacg gcgtcgaatg
     2941 ggtcggccat cgcttcggcg tcgcgcgcac ggccgtgggc acggtgctcg cggccttcgg
     3001 caccgcgctg ccggaaagcg tcgtcacgct ggtggccgtg gtgttcgggc gcacgccggg
     3061 gcagcgcgac atcggcgtcg gcgcggcgct gggcgggccg ctggcgctct cgacgctcgc
     3121 gtacgcggtc gtggggctcg tgttcctggt ggaccggcgc caccgcggcg gcggcatcgt
     3181 gcgcgagttc agcacgcgcg cgctcgccca cgaccagggc tggttcctcg tcgtgttcgt
     3241 ctcgaaggtg gcgctcggcc tggtcgcgtt cgcgtggaag ccgtggctgg gcacgctgtt
     3301 cgtgctggcc tatgcgctgt atgtctggcg cgagctgcgt gccgaggatc ctgccggcgg
     3361 ctgcgagggt gacgacgcgc ccgacgacgc ggacttcgag cggctcaagc tgcggccgca
     3421 cgccgcgaat ccgtccaccg gctgggccgt gctgcagacg acgggcgcgc tcgtggtgat
     3481 cttcctcgcc tcgcagctgt tcgtgcggca gctgggcgtc gtcgggccgg ggctcgggat
     3541 gtcgccgcag ctggtcgcgc tgctgctcag cccgatcgcc accgagctgc ccgagatcct
     3601 gaatgcggtg atctgggtgc gccagggcaa gacggcgctg gcactgggca acatcagcgg
     3661 ggcgatgatg atccaggcga cgatccccag cgcgctgggc atctggttca cgccctggct
     3721 gctcgaccgc ccgctgctgc tggccgcggg cgtgacgatg gcggcgatcg ccgtgaccct
     3781 cgtgctgctg cgccgccacg cgctcaccgc gccgcgactc gtggcgctgg cggggttgta
     3841 cggcgtgttc gcggcgggcg tggcgtggat gtggcggtga cggcccggca gggcgcccgc
     3901 cgcggcctca cttgctcgcg ggcttgagca ggctgcgcac gcgggtgcgc ttggccggca
     3961 gcggcggcat cggtgcgacc gccgccccgg gctcgagctc ggtctccgcg acggccgaga
     4021 tctcgaccgc cgaggccgcg cggtcggcgt agcgcggcgg ccagtccttg agcaccgcgc
     4081 gccgcagcag cgcgacgccg ggacccgccg tgaaggtgcg ctcgaacacg tgcgtgacca
     4141 gctggcgctt gatcttctcc gtgacgatga cggtggagca gcgcgtgccg tagcgaccgt
     4201 ccggcgtgcg cacgaaggcc ggggcgagca cgcgctcgcg ctcgcggccg atgccggtgt
     4261 tgggcagccg gtcgtcgggc gggacgccgc ggtcggacag cgccgcgaac agcatgctgg
     4321 acagcgcgtc cacggagtcg gccgcgtcca gcgcctcgcc gagccgcgcc ttgagcgcct
     4381 cgaccttggg ccacggcgtg tcgagcgccg cgttcgatag cccgaagatg ccgcgctcga
     4441 tgcggcgcgg gatcgcctgc tcgttgctgg cccagaagca ctcgccgcgg cggaagtcgg
     4501 cggcgatcag gttgaacggc gcatagcccg acagcgaggt ctgcatccag aactggtcgg
     4561 ggcgcagctc ggtgcgcagc cagcgtggca cgatgccgcc gcggctgggc gcgtgcgggt
     4621 cgggctcgac gatgcggcgg acgttggtga cgagggccag ccggccggcc gcgctcaggc
     4681 cgagccaggt gccgccggcc gacaggtcgc ggccggcgag gatctgcggg ccgccctcgg
     4741 cgggctgcca ccacgccagc cgggcggcgg ggcggtcgaa gaactcgtcg cggttggcgg
     4801 cgatcaccag cgggaagcgc cggttctcgt cgatcgcgag gcctacgagg cacatgggcg
     4861 gaagagttca gtgtggcgcg cgccgcgcac aagggcggcg agcggggcgg cagcccggtc
     4921 gagcgccgcg aggaaggccg cggtggcggc ggaggccgcg gcgggatccg cgggcagggg
     4981 cagggtgtac gtttccatca gcgtgtgctc gacgtccggg gcgacggcgg caccggacgg
     5041 cggggctgcg ggcgacgcat gctcggtggg cgactcggag gcgttggagc gggtcgacga
     5101 cgcgtcgtcc cgcggcagtg cgcatcgtaa gagcacttgc gcgccgcccg cgcccgcgag
     5161 tgcgggcaat gtgcgctgga attcacgcac cgtcgagatg gcgctgcgaa gatcggccgc
     5221 cgcgaccttg tagtagacgc aggcgagcag cgtcgcgccg ggcacgccgg gagcggtcgt
     5281 gatgtcggtc acgattcgcg gcctcgcccg cggggctatt gcggcgatcg cccggccgtc
     5341 actcggcgtc gatcgccggc aacgcgtagg gcagctcgcg cacggccagg cgcgggccat
     5401 cggcggcacc ggcgtggaac tcgccaccgt cgacgagcgc gcgcttgacc tccacgagcg
     5461 ccgcgtggcg gccgccggcc aggctggccg agagcacgac ctggcccgcc ggctggtcgg
     5521 gttcggcggg cgagaagacc tcggtgcccg cctcgggggc ggcctcgccc tcgaccagca
     5581 ccatgcggcg cttgaccgtg ccgcggtact ggctgcgcgc gacgatctcc tggccggggt
     5641 agcagccctt cttgaagttg acgccgccca ccagctccag gttgaccatc tgcggcacga
     5701 aggcctcggc cgtccgcgcc accacgcgcg gcacgccggc ctgcacctcg agccagttcc
     5761 aggtcgtggc gtccgtggcc gcgggccacg gcggggacgc gtcggccggc gcggcccaca
     5821 gcacccgcgc gcggccgccg
//
LOCUS       DASVLN010000366         1849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_503836, whole genome shotgun sequence.
ACCESSION   DASVLN010000366 DASVLN010000000
VERSION     DASVLN010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503836"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /gene="aroE"
                     /locus_tag="VF453_09015"
     CDS             <1..336
                     /gene="aroE"
                     /locus_tag="VF453_09015"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430406.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7687834.1"
                     /translation="SAAALDAPDEPFDVLVNATAASLAAELPPLPAGTLAPGALALDM
                     MYGPAALPFLQWARAQGAVARDGLGMLVEQAAEAYFTWFGVRPDTFAVLAALRREVDA
                     LAPPAPAAA"
     gene            350..1123
                     /locus_tag="VF453_09020"
     CDS             350..1123
                     /locus_tag="VF453_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7687835.1"
                     /translation="MRHALRLVGMLAACALALQLYFLLRIALMLVVQPESTTFQRSEI
                     WRLLVDKHEIAWSQESVPGERISDNLRRAVIASEDAGFYDHHGVEWGAVEKAWNRNEK
                     AEARVEAQQSRAAAAARRNPNGAAARQRVAVAKVVGGSTISQQLAKNLFLSGERTILR
                     KGQELVITEMLEAVLGKDRILTTYLNNVEWGEGVFGAEAASEHYFRAHAAFLAPSQAA
                     RLAVMLPAPKRFEKTPSSPYVLGRSATIAARMGAVELEK"
     gene            1188..1784
                     /locus_tag="VF453_09025"
     CDS             1188..1784
                     /locus_tag="VF453_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687836.1"
                     /translation="MEADRERQEIAQAAARLIVEEGLDYASARRRAMQAHGGRLREAP
                     ANELIEDEVRARLSLFHADSQPQELRILREAALRWMLRLAEFRPHVGGAVWRGTATRL
                     SPVLIDLYCDDTKAPEIALLNLGVDYETASEPIGRERHALTLVISERSRELDDWLTIH
                     LNVLDLDDLRGALKPDARGRSWRGDAQGLRRRLEEGEA"
BASE COUNT          265 a          679 c          674 g          231 t
ORIGIN      
        1 tcggccgccg cgctcgacgc gccggacgag cccttcgacg tcctcgtcaa cgcgaccgcc
       61 gcgagcctgg ccgccgaact cccgccgctg ccggccggca cgctggcgcc gggcgcgctg
      121 gcgctggaca tgatgtacgg ccccgccgcg ctgcccttcc tgcaatgggc gcgcgcccag
      181 ggcgcggtcg cgcgagacgg gctgggcatg ctggtggagc aggccgcgga ggcctacttc
      241 acatggttcg gggtgcggcc ggacaccttc gcggtgctcg cggcgctgcg gcgcgaggtc
      301 gacgcgctcg caccgcccgc cccggccgcc gcctgaggag gcgtcccgaa tgcgccacgc
      361 cctgcgtctc gtcggcatgc tcgcggcgtg cgcgctcgcg ctgcaactgt acttcctgct
      421 gcgcatcgcc ctcatgctcg tggtgcagcc ggagtcgacg accttccagc gcagcgagat
      481 ctggcgcctg ctggtcgaca agcacgagat cgcctggtcg caggagtcgg tgcctggcga
      541 acgcatctcc gacaacctgc gccgcgcggt catcgcgagc gaggacgccg gcttctacga
      601 tcaccacggc gtcgaatggg gcgccgtcga gaaggcctgg aacaggaacg agaaggccga
      661 ggcccgcgtg gaggcccagc agagccgggc cgccgcggcc gcccgccgca acccgaacgg
      721 cgcggccgcg cggcagcgcg tggccgtggc caaggtggtg ggcggctcga cgatctcgca
      781 gcagctcgcg aagaacctgt tcctgtccgg cgagcgcacc atcctgcgca agggccagga
      841 gctcgtgatc accgagatgc tcgaggcggt gctgggcaag gatcgcatcc tcacgaccta
      901 cctgaacaac gtcgagtggg gcgagggcgt gttcggcgcc gaagccgcct ccgagcacta
      961 tttccgcgcc cacgccgcct tcctcgcgcc gagccaggcc gcccgcctgg cggtgatgct
     1021 gccggcaccg aagcgcttcg agaagacgcc ctcctcgccc tacgtgctcg ggcgctcggc
     1081 gacgatcgcg gcgcgcatgg gggcggtcga gctcgagaag tagcggcggc atgcgacccc
     1141 ccccccgcgg gcacgtgccg ccgcgagcgc gtaaagtggc cgacatcatg gaagcggatc
     1201 gcgaacgaca ggaaatcgcc caggccgccg cgcggttgat cgtggaggag gggctggatt
     1261 acgcgagcgc ccgccgccgc gcgatgcagg cgcacggcgg tcggctgcgg gaggcaccgg
     1321 ccaacgagct gatcgaggac gaggtccgcg cccgtctctc gctgttccat gccgacagcc
     1381 agccgcagga actgcgcatc ctgcgcgagg ccgcgctgcg ctggatgctg cggctcgcgg
     1441 agttccgccc gcatgtcggc ggcgcggtct ggcgcggcac ggcgacgcgg ctgtcgccgg
     1501 tcctgatcga cctttactgc gatgacacca aggcgcccga gatcgcgctg ctgaacctgg
     1561 gcgtcgacta cgagacggcc agcgagccga tcggccgcga gcgccatgcg ctgacgctgg
     1621 tgatctcgga gcgcagccgg gaactcgacg actggctgac gatccacctg aacgtgctcg
     1681 acctcgacga cctgcgcggc gcgctcaagc ccgacgcccg cggccgcagc tggcgcggcg
     1741 acgcccaggg gctgcgccgc cgactcgagg aaggcgaggc atgagctcgc cagcgcccga
     1801 ctcggcggcc gccgaccccg gaggccggcg gaccggcgtc cgcacgccg
//
LOCUS       DASVLN010000367         2948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_288527, whole genome shotgun sequence.
ACCESSION   DASVLN010000367 DASVLN010000000
VERSION     DASVLN010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288527"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="VF453_09030"
     CDS             complement(<1..1343)
                     /locus_tag="VF453_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687837.1"
                     /translation="MPIIAPVLDDRSFEDLFLELRNRIPAYTPEWTDYQDSDPGITLL
                     QLFAYLGEGLQFRFNQIPEATQLAFLKLLGVPMIPPRPAQALVRFKSKVPNGVSLYAG
                     DQLKAGRTLYTLSQDATAWPLDCVAVARQALLSEEDLADNAKVSAWIAGLDKEVGDAV
                     QASVDALDLADDDHVAPYQVATLPSDGTGPAIDFSTSVDGVMWIAVLVDPDANIPPAR
                     ILDPLLGLQRPDGRAISLSLGFAPAAWFPTIDQSPACAAGNGPSLSWQASLAKPRTDG
                     APDFLNLRVGGDTTAGFTQAGVVRVDLPIDPTPLGVPSAPVELAGTGSFPPTLDDAQA
                     ERLWFWLRVWRADGSRIGPVQLVSLNTAGCEQVVAAAPELLGTGTGQPGQVFTLANAP
                     VLLDARWPVQLQVEESGTWTDWARVDDFDASTPDDRHFVVDPEGATVSFGDGAAGR"
     gene            complement(1348..1755)
                     /locus_tag="VF453_09035"
     CDS             complement(1348..1755)
                     /locus_tag="VF453_09035"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX7687838.1"
                     /translation="MNAGPLDLYRRLGTGLGQPLVPGSTGALPRVGGPEKVRQSILTI
                     LDTDPGERVMRPGFGCGLRRYLMAPDNPATRAGIEREVTTALALWEPRVKLVGVAVSP
                     TDDRSQVLIEIRYAHVLDGRQDILVYPFYLEQA"
     gene            complement(1752..>2948)
                     /locus_tag="VF453_09040"
     CDS             complement(1752..>2948)
                     /locus_tag="VF453_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687839.1"
                     /translation="VAAVRARQHALATAAGLVDWDALVALRESQLAQQRAGLDAWFRA
                     HGVGVAIDGWFPTLQEGRPSPLYGDWRPLDDAQQLADVTSGEQTYPLFPLVPDPRDST
                     HDAAGRTLFYGNVPTASLQHDTNGGSHFDDHTTYELRCFVRRHHNCPPKVGKKPDCSG
                     PLAWSQPTEAFRLAAAFDVVGAANRPITIRMPDLRDLAAQVAARPRGRLSPVRVVQPQ
                     HLSPQTDGSGVTGGAMGGSATCSFSIPLITIVAMFVLNLFLPIVVFVFQLWFLLALRF
                     CIPPSIQASAGVDAALAATPPGVDLDADFAVAVAGTPELAMQLNALLKGSVGAMLQQD
                     SGHAPSLGTLSNAALGPLDQTLADNARLAAGADGALPPPPPVGTPLVYEPQVEPVASS
                     RTGASA"
BASE COUNT          406 a         1004 c         1139 g          399 t
ORIGIN      
        1 cggcccgccg cgccgtcgcc gaaggacacg gtcgccccct ccgggtcgac gacgaagtgg
       61 cggtcgtccg gggtcgaggc gtcgaagtcg tcgactcgtg cccagtccgt ccaggtgccg
      121 ctctcctcga cctgcaactg cacgggccag cgcgcatcga gcagcaccgg cgcgttggcg
      181 agcgtgaata cctggccggg ctggccggtg ccggtgccga gcagctcggg tgcggccgcg
      241 acgacctgct cgcagccggc ggtgttcagg ctcacgagct gcaccgggcc gatgcggctg
      301 ccgtccgcgc gccagacgcg cagccagaac cacaggcgct cggcctgcgc gtcgtccagc
      361 gtgggcggga agctgccggt gccggcgagc tccaccgggg ccgagggtac ccccaagggt
      421 gtcgggtcga tgggcaggtc gacgcgcacg acgcctgcct gcgtgaagcc ggcggtcgtg
      481 tcgccgccca cgcgcaggtt caagaagtcg ggcgcgccgt ctgtcctggg cttggcgagc
      541 gaggcctgcc acgacagcga gggcccgttg cccgcggcac atgcgggcga ctggtcgatg
      601 gtggggaacc aggccgcggg cgcgaagccc agcgacagcg agatcgcccg cccgtcgggc
      661 cgctgcaggc ccagcagcgg gtcgaggatg cgcgcgggcg ggatgttcgc gtcgggatcg
      721 accagcactg cgatccacat gacgccgtcc acgctcgtcg agaagtcgat cgccggaccg
      781 gtgccgtcgg agggcagcgt ggccacctgg tagggcgcga cgtggtcgtc gtcggcgagg
      841 tcgagcgcgt cgacgctggc ctgcacggcg tcgccgactt ccttgtccag gcccgcgatc
      901 cacgccgaga ccttggcgtt gtcggcgaga tcctcctcgc tgagcagcgc ctgccgcgcg
      961 accgcgacgc agtccagcgg ccacgccgtg gcgtcctgcg acagcgtgta cagcgtgcgg
     1021 ccggccttga gctggtcgcc ggcgtacagg ctcaccccat tgggcacctt gctcttgaag
     1081 cgcaccagcg cctgcgccgg ccgcggcggg atcatcggca cgcccagcag cttcaggaac
     1141 gccagctgcg tcgcctcggg gatctggttg aagcgaaact gcaggccctc gccgaggtag
     1201 gcgaacagct gcagcagcgt gatgccgggg tcgctgtcct ggtagtcggt ccattcgggc
     1261 gtgtaggccg ggatgcggtt gcgcagctcg aggaacaggt cttcgaagct gcggtcgtcg
     1321 aggacggggg cgatgatcgg catggcttca ggcctgttcg aggtagaacg ggtagacgag
     1381 gatgtcctgg cggccgtcga ggacgtgggc gtaccggatc tcgatcagca cctgcgagcg
     1441 gtcgtcggtg gggctcaccg cgacccccac cagcttcacg cgcggctccc aaagcgccag
     1501 cgcggtcgtg acctcgcgct cgatgcccgc gcgcgtggcc gggttgtcgg gcgccatcag
     1561 gtagcgccgc aggccgcagc cgaagccggg tcgcatcacg cgttcgccgg ggtcggtgtc
     1621 gaggatcgtg aggatggact ggcgcacctt ctccgggccg ccgacgcgcg gcagtgcgcc
     1681 ggtcgagccg ggcacgaggg gctgccccag gcccgtgccc agccggcgat agagatcgag
     1741 ggggccggcg ttcatgcgct tgctcccgtg cggctcgacg ccacgggctc gacctgcggc
     1801 tcatacacca ggggggtacc gaccggcgga ggcggcggca gcgcgccgtc cgcgcccgcg
     1861 gcaaggcgcg cgttgtcggc gagggtctgg tcgagcggcc cgagcgccgc attgctcagc
     1921 gtgccgagcg acggcgcgtg gccggagtcc tgctgcagca tcgcgccgac gctgcccttc
     1981 agcagcgcgt tcaactgcat cgcgagctcg ggcgtgccgg ccacggcgac ggcgaagtcg
     2041 gcgtcgaggt ccacgcccgg cggcgtcgcg gccagcgcgg cgtcgacgcc ggccgaggcc
     2101 tggatcgacg gcgggatgca gaagcgcagc gcgagcagga accagagctg gaagacgaac
     2161 acgacgatcg gcaggaacag gttgagcacg aacatcgcga cgatcgtgat gagcgggatc
     2221 gagaagctgc aggtcgcgct gccgcccatc gcgccgccgg tcacgccgct gccgtcggtc
     2281 tgcggcgaca ggtgctgcgg ctgcacgaca cgcacggggg agagtcgccc ccgtggccgc
     2341 gccgccacct gcgccgcgag gtcgcgcagg tcgggcatgc ggatggtgat cggacggttg
     2401 gccgcgccga cgacgtcgaa ggccgctgcg aggcggaagg cctcggtcgg ctggctccat
     2461 gcgagcggcc cgctgcagtc cggcttcttg ccgaccttgg gcgggcagtt gtggtgacgg
     2521 cggacgaagc agcgcagctc gtaggtggtg tggtcgtcga agtgcgatcc gccgttcgtg
     2581 tcgtgctgca ggctcgcggt gggcacgttg ccgtagaaca gcgtgcggcc ggcggcgtcg
     2641 tgggtggaat cgcgcggatc ggggacgagc gggaacagcg gataggtctg ctcgcccgag
     2701 gtgacgtccg cgagctgctg cgcgtcgtcc agcggccgcc agtcgccgta cagcgggctc
     2761 ggccggcctt cctgcagcgt ggggaaccag ccgtcgatcg cgacgccgac gccatgcgcg
     2821 cggaaccacg cgtcgagccc cgcacgctgt tgcgccagct gcgattcccg cagcgcgacg
     2881 agcgcgtccc agtcgacgag gccggccgcc gtcgcgagcg catgctggcg cgcgcgcacc
     2941 gcggcgac
//
LOCUS       DASVLN010000368         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_180647, whole genome shotgun sequence.
ACCESSION   DASVLN010000368 DASVLN010000000
VERSION     DASVLN010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180647"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VF453_09045"
     CDS             <1..455
                     /locus_tag="VF453_09045"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEX7687840.1"
                     /translation="ATLSVTLAALVVAAVGQVAWPQRDAIAARWPSTMPMWTALCDLA
                     GCRIEPPRALASLALDGSSLTRTETEHVLLFSADLHNRSGQAVRMPSFDLTFMDLNGA
                     IVARKVLSPEQIGIRQPALPPDAEVHVHARLQVGTLDASGFNADLFYP"
     gene            473..1378
                     /locus_tag="VF453_09050"
     CDS             473..1378
                     /locus_tag="VF453_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEX7687841.1"
                     /translation="MASLVCGSLAFDTITVFPGRFADQILPEQLHILNVSFLVPTLRR
                     EFGGCAGNIAYTLKLLGGEPVVLAALGNDGQQYLAHMERLGISRESVHVTDDNYTAQA
                     IIITDAANNQITAFHPGAMMQAHETGLPARGDIALAIIGPDGRDAMMRRARDLAAAGI
                     PFIFDPGQGLPMFDGDDLRAFVAQATWVTVNDYEARMLTERLGTDLATLSRSHLRGVV
                     VTLGEQGCEVWVQGERTHVPGVQATAVVDPTGCGDAFRGALLYGLELGWPLVDCVTLG
                     NRIGALKIAHRGGQNHAIDRAALGL"
     gene            1467..>1756
                     /locus_tag="VF453_09055"
     CDS             1467..>1756
                     /locus_tag="VF453_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX7687842.1"
                     /translation="MSARPFLLVDAFTGAPLGGNPCAVVLDADGLDDARRQALAREFN
                     QAETAFVSRGADGADFSVQLFHARRGDPARGASDDRDRRRAPPRRARAGRPA"
BASE COUNT          235 a          671 c          608 g          242 t
ORIGIN      
        1 gggcgacgct gtcggtgacg ctggcggcgc tcgtcgtcgc ggccgtcggg caggtcgcct
       61 ggccacagcg cgacgcgatc gccgcgcgct ggccgtcgac gatgccgatg tggacggcgc
      121 tgtgcgacct ggccggctgc cgcatcgagc ccccgcgcgc gctcgcgagc ctcgcgctgg
      181 acggcagctc gctcacgcgc acggagaccg agcacgtgct gctgttctcc gccgacctgc
      241 acaaccgctc cggccaggcc gtgcgcatgc cttcgttcga tctcacgttc atggacctga
      301 acggcgcgat cgtcgcgcgc aaggtgctct cgcccgagca gatcggcatc cgccagcccg
      361 cgctgccgcc cgacgccgaa gtgcacgtgc acgcgcggct gcaggtgggc acgctcgacg
      421 cgtccggctt caacgccgac ctgttctatc cctgagaccc gagaaagcac ccatggccag
      481 cctcgtctgc ggatcgctcg cgttcgacac gatcaccgtc ttccccggcc gcttcgccga
      541 ccagatcctg ccggagcagc tgcacatcct gaacgtgtcc ttcctcgtgc cgacgctgcg
      601 gcgcgagttc ggcggctgcg ccggcaacat cgcctacacg ctcaagctcc tgggcggcga
      661 gcccgtcgtg ctcgcggcgc tgggcaacga cggccagcag tacctcgcgc acatggaacg
      721 cctgggcatc tcgcgcgaga gcgtgcacgt caccgacgac aactacacgg cgcaggcgat
      781 catcatcacc gacgccgcca acaaccagat caccgccttc cacccgggcg cgatgatgca
      841 ggcgcacgag accggcctgc cggcgcgcgg cgacatcgcg ctggccatca tcggccccga
      901 cggccgcgac gcgatgatgc gccgcgcgcg cgacctcgcg gccgcgggga tccccttcat
      961 cttcgacccg ggccagggcc tgccgatgtt cgacggcgat gacctgcgcg ccttcgtcgc
     1021 ccaggcgacg tgggtcacgg tcaacgacta cgaggcccgc atgctcaccg aacgcctggg
     1081 caccgacctc gcgacgctgt cgcgctcgca tctgcgcggc gtcgtcgtga cgctgggaga
     1141 acagggctgc gaggtctggg tgcagggcga gcgaacgcac gtcccgggcg tgcaggcgac
     1201 cgcggtggtc gacccgaccg gctgcggcga cgcgttccgc ggcgcgctgc tgtacggcct
     1261 ggagctcggc tggccgctgg tcgactgcgt gacgctgggc aaccgcatcg gcgccctcaa
     1321 gatcgcgcat cggggcggcc agaaccacgc gatcgaccgc gccgcgctcg ggctgtgacg
     1381 gcccgcatcc atcgcgcagg gcgacgggcg tccgccgcgc gggccacctg gcgtccggtg
     1441 ggccggccgc ggcgtgcggc agcgtcgtga gcgcacggcc cttcctgctc gtcgacgcgt
     1501 tcacgggcgc gccgctcggc ggcaacccgt gcgcggtggt gctcgacgcc gacggtctcg
     1561 acgacgcccg ccgccaggcg ctcgcgcgcg agttcaacca ggcggagacc gccttcgttt
     1621 cgcggggcgc cgacggcgcg gacttctcgg tgcagctatt tcacgcccgc cgaggagatc
     1681 ccgctcgcgg ggcatccgac gatcgcgacc gtcgtcgcgc tcctccacgt cgggcgcgtg
     1741 caggccggcc cgcaag
//
LOCUS       DASVLN010000369         1882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_394721, whole genome shotgun sequence.
ACCESSION   DASVLN010000369 DASVLN010000000
VERSION     DASVLN010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394721"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VF453_09060"
     CDS             <1..1281
                     /locus_tag="VF453_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7687843.1"
                     /translation="LASLDDGIAAMRGGDLSARRVAGDDAEFDGIARALEDMNARMSA
                     LVADIRSGATFVSQAGASLAAGTGDLARRTERQACSVEQSSANVQQLGASVQRNADDA
                     RRVEQLAAQVHAVAESGGGRMHQAVASMHAIRSGSERVQDIIGVIDGIAFQTNILALN
                     AAVEAARAGDNGRGFAVVASEVRSLALRSSKAAGEIKSLILASSEQIDAGVRNIDEVG
                     GLLAEVVAGIGRVAGDVRAITEAAGQQSLGLQEMGVAIRGIDEIARENAGLVERTSTA
                     SAGLEERARGLAASVQGFRLRQGTADEAHALVGRAVSLFRRRGRACLDEITDAGAFAD
                     RDMYVFAWDRELVYHAFAGKPHNVGKCAREILGTDTRRLAHDVWAAADQGGGWVDYDF
                     LNPATGTVAPKTSFVVPVADDLVLGCGVYKTVQA"
     gene            complement(1331..1792)
                     /locus_tag="VF453_09065"
     CDS             complement(1331..1792)
                     /locus_tag="VF453_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687844.1"
                     /translation="MRPFILDATLAALAALAVSACAALAPTARETPMDHDESSAPPRV
                     MAKRAGPPKVPPIRIGDLRFEQAAPPRAETSDVQRLGWLSAFRGDSDERLWSVKVYPI
                     VYDPHMESDVRDVWFAHMSASADGRQVLIDNERGERYAVDVAAPHAVHRLP"
BASE COUNT          257 a          674 c          714 g          237 t
ORIGIN      
        1 ctggcgtcgc tcgacgacgg gatcgccgcg atgcgcggcg gcgacctgag cgcgcggcgc
       61 gtggcggggg acgacgccga gttcgacggc atcgcgcgcg cgctcgagga catgaacgcg
      121 cggatgtccg cgctggtcgc cgacatccgc agcggcgcga ccttcgtctc gcaggccggc
      181 gcgtcgctcg ccgccggtac cggcgacctc gcgcgccgta ccgagcgcca ggcgtgcagc
      241 gtcgaacagt cgtcggccaa tgtgcagcag ctgggcgcct ccgtgcagcg caacgccgac
      301 gatgcgcgcc gtgtcgagca gctggccgcg caggtgcacg ccgtcgccga atccggcggc
      361 ggacggatgc accaggcggt ggcgtcgatg catgccatcc gcagcggctc ggagcgcgtg
      421 caggacatca tcggcgtgat cgacggcatc gcgttccaga ccaacatcct ggcgctcaat
      481 gccgcggtcg aggcggcgcg ggccggcgac aacggccgcg gcttcgcggt ggtcgcgtcc
      541 gaggtccgaa gcctcgccct gcgctcgagc aaggccgcgg gcgagatcaa gtcgctgatc
      601 ctcgcctcga gcgagcagat cgacgccggc gtgcgcaaca tcgacgaggt cggcggactg
      661 ctggcggagg tcgtcgcagg catcggccgc gtcgccggcg acgtgcgcgc gatcaccgag
      721 gccgcgggcc agcagagcct gggcctgcag gagatgggcg tggcgatccg cggcatcgac
      781 gagatcgcgc gcgagaacgc cggcctggtc gagcgcacgt ccacggcgtc ggccgggctc
      841 gaggagcggg cgcgcggcct ggccgcgagc gtgcagggat tccggctgcg ccaggggacg
      901 gccgacgagg cgcatgcgct cgtcggacgc gcggtgtccc tgttccgtcg ccgcggccgc
      961 gcgtgcctgg acgagatcac cgacgccggc gcattcgccg atcgcgacat gtatgtcttc
     1021 gcctgggatc gcgagctcgt ctaccacgcg ttcgccggca agccgcacaa cgtcggcaag
     1081 tgcgcccgcg aaatcctcgg caccgacacg cggcgcctcg cgcacgacgt gtgggccgcc
     1141 gccgaccagg ggggcggctg ggtcgactac gacttcctca acccggcgac aggcacggtc
     1201 gcgcccaaga cctcgttcgt ggtgccggtg gccgacgacc tcgtgctcgg gtgcggcgtc
     1261 tacaagaccg tccaggcctg atcgaatcgg cgtcccctcc ggcgtcgccg accggtgctc
     1321 aggtcggcgc tcagggcagg cgatgcacgg cgtgcggcgc cgcgacgtcg accgcgtagc
     1381 gctccccgcg ctcgttgtcg atcagcacct gccggccgtc cgccgaggcg gacatgtgcg
     1441 cgaaccacac gtcccgcacg tcggattcca tgtgcgggtc gtacacgatc ggatagacct
     1501 tcaccgacca caggcgttcg tcgctgtcgc cgcggaaggc gctgagccag ccgaggcgct
     1561 ggacgtccga cgtctccgcg cggggcgggg cggcctgctc gaaacgcagg tcgccgatcc
     1621 ggatcggcgg caccttcggc ggcccggcgc gcttggccat gacgcgtggc ggggcggatg
     1681 actcgtcatg gtccatgggg gtctcccggg cggtcggcgc gagcgcggcg caggccgaga
     1741 cggccagggc ggccagggcg gccagcgtcg cgtcgaggat gaaggggcgc atcgcgcgag
     1801 gctaccattc cggcgccgcg cagatcccgc gcaggcgccg cgcgcatcgg cgccgtcagg
     1861 ccacgcgccg cccgcgcgac ca
//
LOCUS       DASVLN010000370         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_394745, whole genome shotgun sequence.
ACCESSION   DASVLN010000370 DASVLN010000000
VERSION     DASVLN010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394745"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VF453_09070"
     CDS             <1..793
                     /locus_tag="VF453_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7687845.1"
                     /translation="GETAADVAARAAQSLEAWLDAHHDETAALIVEPLVQCATGMAMH
                     DPAYLRAARALCDRYGVHLVVDEIATGFGRTGTMFAHQQAGIRPDFVCLSKGLTGGTM
                     ALAVVLATDAVYEAFYDDDTARGFLHSHSYTGNPLACRAALATLELFEREDVIAANRE
                     RGDMIDRHLRPLHAHPRLRHARRLGMIWAWDVADAPADFARRYARHALAHGLLLRPIG
                     ATLYAMPPYVLGEAEAAHLGRGALAALEATLAESADPRVDAKALP"
     gene            790..1470
                     /gene="bioD"
                     /locus_tag="VF453_09075"
     CDS             790..1470
                     /gene="bioD"
                     /locus_tag="VF453_09075"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX7687846.1"
                     /translation="MTTSPRAWFVVGTDTGVGKTHAVGALLHALARRHARVVGMKPVA
                     AGGVESPAGFSNEDSLAHRAASTIAVPPALDNPILLPEPLSPHLAAARAGVEVDFDRI
                     LAAFAQLRARADAVVVEGAGGFLVPMSETRTGADLAAALGLPIVLVVGMRLGCLNHAL
                     LTAEAIRARGLALAGWIANRVDPHFLCPDENLAYLRSHLGAPLWADLPYAATPDARAD
                     ADRFTLPD"
BASE COUNT          186 a          650 c          532 g          207 t
ORIGIN      
        1 cggcgagacc gcggccgacg tggccgcccg cgccgcgcag tcgctcgagg cctggctcga
       61 cgcccaccac gacgagaccg cggccctgat cgtcgagccc ctcgtgcagt gcgcgaccgg
      121 catggccatg cacgacccgg cctacctgcg cgccgcgcgc gcgctgtgcg accgctacgg
      181 cgtgcacctg gtcgtcgacg agatcgccac cggcttcggc cgcaccggca cgatgttcgc
      241 gcaccagcag gccggcatcc ggcccgactt cgtgtgccta tccaagggcc tgacgggcgg
      301 cacgatggcg ctggcggtgg tgctcgcgac cgatgcggtc tacgaggcct tctacgacga
      361 cgacaccgcg cgcggcttcc tgcactcgca ctcgtacacc ggcaatcccc tcgcatgccg
      421 cgcggcgctg gcgacgctgg agctgttcga gcgcgaggac gtcatcgccg ccaaccgcga
      481 gcgcggcgac atgatcgacc gccacctgcg cccgctgcac gcccatcccc gccttcgcca
      541 tgcgcgtcgc ctcggcatga tctgggcctg ggacgtcgcc gacgcaccgg ccgacttcgc
      601 ccgccgctac gcccgccatg cgctcgccca cggcctgctg ctgcggccga tcggcgcgac
      661 gctgtatgcg atgccgccct acgtgctggg cgaggccgag gctgcgcacc tgggccgcgg
      721 cgcgctggcg gcgctggagg cgacgctcgc ggaatcggcc gatccgcgcg tcgacgcgaa
      781 ggcgctgcca tgacgacgag cccccgcgcc tggttcgtcg tcggcacgga caccggggtc
      841 ggcaagaccc acgccgtcgg cgcgctgctg catgcgctcg cgcgccggca tgcgcgcgtc
      901 gtcggcatga agccggtcgc ggccggcggc gtcgagtccc cggcgggctt ctcgaacgag
      961 gactcgctcg cccatcgtgc cgcctcgacg atcgcggtgc cgcccgcgct cgacaacccg
     1021 atcctgctgc ccgagccgct gtcaccccac ctcgcggccg cgcgcgccgg cgtcgaggtc
     1081 gacttcgacc gcatcctcgc ggcgttcgcg cagctgcgcg cgcgcgccga tgcggtcgtg
     1141 gtcgagggcg ccggcggttt cctcgtgccg atgtccgaga cgcgcaccgg cgccgacctc
     1201 gccgccgcgc tcggcctgcc catcgtgctg gtcgtgggca tgcgcctggg ctgcctcaac
     1261 cacgcactgc tgaccgccga ggcgatccgc gcgcgcgggc tcgcgctggc cggctggatc
     1321 gccaaccgcg tcgacccgca cttcctgtgc cccgacgaga acctcgccta cctgcgcagc
     1381 catctcggcg cgccgctgtg ggccgacctc ccctacgccg cgacgcccga cgcgcgcgcg
     1441 gacgccgacc gattcacgct gcccgactga cgcccgtcgc ggcgcgccac gacatgaccg
     1501 acaccgcttc ctcctggctc gacgagctgc ccgcccggct ggccgaactc gacgccgccg
     1561 acctcacgcg ccgcc
//
LOCUS       DASVLN010000371         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_504042, whole genome shotgun sequence.
ACCESSION   DASVLN010000371 DASVLN010000000
VERSION     DASVLN010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504042"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1364)
                     /locus_tag="VF453_09080"
     CDS             complement(30..1364)
                     /locus_tag="VF453_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7687847.1"
                     /translation="MTRDSVLTAKAADAAAVPAATGAGRRSPITWVPTLYFAQGVPFF
                     VVMAIATLLFKDLGVPNADVTRWTNTLGLAWAVKPLWSPFLEAVPSKKLLVILFQLVG
                     AATLGLVALALNVPHSVALVLAILGFTAFGSATHDIASDGLYIASLSDRQQAEYAGWQ
                     GAFFNAARLFARGGVLVLVGMLEKTMEAAHAWSAVFVLLAAVMAALAAYHVWALPDTR
                     GAEPPARDLGRIARTLWEVVVDFFRKPGIWFAIAFIILFRAGEGQIQSVGPLFLKDAR
                     AVGGLGLTTDQIGVAYGTAATIAFVLGSVAGGYFTSWLGLRRAMVWLVVAMNLPNLVY
                     WWLSVARPSDLTQIAAGLSVEMFGYGFGFVGIILFIMQVVAKGRFTTAHYALGTGVMQ
                     LGFILSGTWSGEIQTRLGYQDFFLWVLACAVPVLVLSFFLPHRRADDAQAGA"
     gene            complement(1465..>1893)
                     /locus_tag="VF453_09085"
     CDS             complement(1465..>1893)
                     /locus_tag="VF453_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX7687848.1"
                     /translation="PAVPPARDARLRVVTQASVECDGVSEAGLPAERVAALFAGFGDV
                     QFVRVDDLRAIDASTFPADGRVNVLVSNHRTRFGDGAAAARPDLHLALWNPFQLLDVA
                     APSVVTWGYADGALDALQAWLEGRAAAPGRSPVKLSPRAA"
BASE COUNT          303 a          686 c          685 g          219 t
ORIGIN      
        1 cggcgtgccg catcgcatgc ggcgccatct caggcaccgg cctgcgcatc gtcggcgcga
       61 cggtgcggca ggaagaacga cagcacgagc accggcaccg cgcacgcgag cacccagagg
      121 aagaagtcct ggtagccgag gcgggtctgg atctcgccgc tccacgtgcc cgagaggatg
      181 aagcccagct gcatcacgcc ggtgcccagc gcgtagtggg cggtcgtgaa gcggcccttg
      241 gccaccacct gcatgatgaa caggatgatg cccacgaagc cgaagccgta gccgaacatc
      301 tccaccgaca ggccggcggc gatctgcgtg aggtcgctcg ggcgcgcgac gctcagccac
      361 cagtagacga ggttgggcag gttcatcgcg acgaccagcc acaccatcgc gcgccgcagg
      421 cccagccacg acgtgaagta gccgcccgcg acgctgccca gcacgaacgc gatcgtcgcg
      481 gcggtgccgt acgcgacgcc gatctggtcg gtcgtcaggc ccaggccgcc gacggcgcgc
      541 gcgtccttca ggaacagcgg gcccaccgac tggatctggc cctcgccggc gcggaacagg
      601 atgatgaacg cgatcgcgaa ccagatgccg ggcttgcgga agaagtcgac gacgacctcc
      661 cacagcgtgc gcgcgatgcg cccgaggtcg cgcgccggcg gctccgcgcc gcgcgtgtcg
      721 ggcagcgccc acacgtggta cgcggcgagc gccgccatca ccgcggcgag cagcacgaag
      781 accgcgctcc aggcgtgcgc ggcctccatc gtcttctcca gcatgcccac gagcacgagc
      841 acgccgccgc gcgcgaacag ccgcgccgcg ttgaagaacg cgccctgcca gccggcgtac
      901 tcggcctgct gcctgtccga caggctggcg atgtacaggc cgtcggaggc gatgtcgtgc
      961 gtcgccgagc cgaaggccgt gaagcccagg atcgccagca ccagcgccac gctgtgcggg
     1021 acgttgagcg ccagcgcgac caggccgagc gtcgccgcgc cgaccagctg gaagaggatc
     1081 acgagcagct tcttgctcgg caccgcctcc aggaacgggc tccacagcgg cttgacggcc
     1141 caggcgaggc ccagcgtgtt ggtccagcgc gtgacgtccg cgttgggcac gcccaggtcc
     1201 ttgaacagca gcgtcgcgat cgccatcacg acgaagaacg gcacgccctg cgcgaagtag
     1261 agcgtgggca cccaggtgat gggcgagcgc cggccggcgc cggtcgcggc cgggacggcg
     1321 gccgcatcgg ccgccttggc ggtcaggacg gaatcacggg tcaatggggg cctctttcct
     1381 ggcgacgagg gtgcgcgggt ggacacgtcg gcctcgcctg gtctcttggt cgtcgtcgtg
     1441 ggcccgacgc agcttacgcg gcgctcacgc cgcccgtgga gagagcttca ccggactgcg
     1501 ccccggtgcc gccgcgcggc cctcgagcca ggcctgcagc gcgtcgagcg cgccgtcggc
     1561 atagccccag gtcacgaccg acggcgccgc gacgtcgagc agctggaacg gattccacag
     1621 cgcgaggtgc aggtccggcc gcgcggcggc cgcgccgtcg ccgaagcgcg tgcggtggtt
     1681 cgagacgagg acgttgacgc ggccgtcggc ggggaaggtc gacgcgtcga tcgcgcgcag
     1741 gtcgtcgacg cgcacgaact gcacgtcgcc gaagcccgcg aacagcgccg ccacgcgctc
     1801 ggccggcagg ccggcctcgg agacgccgtc gcattcgacg ctcgcctgcg tcacgacgcg
     1861 cagcctcgcg tcgcgcgcgg gcggcacggc cgg
//
LOCUS       DASVLN010000372         4814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_180896, whole genome shotgun sequence.
ACCESSION   DASVLN010000372 DASVLN010000000
VERSION     DASVLN010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180896"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VF453_09090"
     CDS             complement(<1..162)
                     /locus_tag="VF453_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7687849.1"
                     /translation="MSAANRFVWYELMTGDQPAAVDFYRRVVGWSAQDAGMPGMPYTL
                     LLAGAARVAG"
     gene            417..3290
                     /locus_tag="VF453_09095"
     CDS             417..3290
                     /locus_tag="VF453_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HEX7687850.1"
                     /translation="MQRATPSRLPATARATLALLAAAAPASASSSGVVISQVYGGNGN
                     TYASDYVELFNAGASVVSLSGWSIQYASATGSGTFASNGVTTLNGTLQPGQHWLVRLA
                     TTTGPAIPTPDTSATAPNISASSGKLVLVDNSNGLPCNGSSASPCTAVASQIVDLVGY
                     GSANYFEGAAAPGISSTTALFRANGGCTDTGDNSADFTAGAPAPRNSASPLAACAGGG
                     GSPTATPAAIYQIQGNGAKSPYNGQLVQTQGVVTRVNSNGYFLQDLTGDNDPSTSDGV
                     FVYTGGTPTAAAGQLLQLTATVSEFNTGAAANADTAAHTVTELTNPSHVSVLGTGYTI
                     APVLVTLPLASEDDMERYEGMLVTLRGPLTVQQNYFLGRFGELTLATGGRLQTPTNVH
                     RPGPDANALNADNLRRSILLDDGNNAQDPDPTPYIGADDTVRAGDTVAQVTGVIDYGL
                     TTATNTDPGLYKIHPTQPVAFTRANPRTAAADDVGGNVRVASANVENFFTTLDDGRTS
                     CPPSNTPNDCRGANDANEFSRQRAKVVDELAGLNADVVALMELQNNGATAIQSLVDGL
                     NAKLGAPVYARVPDAATGGGSDAIKVGMIYKPSTLALAGPALSDTAAINNRAPMAQTF
                     RLPNGEKFNVIANHLKSKSCSDFTPGSGDADSGDLQGCFNARRVQQADELRGFVNTVQ
                     AASGTADTLLVGDFNAYAKEDPVVELTSNGFVDVIGRWIANGYSYVFNGTSGRLDQAL
                     ATPTLAAKTTGATEWHINADEPEIIDYNEEFKKPACATCGRDLYSDSPYRASDHDPLV
                     VGLNIVHAISGTAGRDTIVGTPGDDVITGGAGADTLTGNGGRDVFVYASMRDAADTIT
                     DFQPGDDRLDLAQLLASIGANPATALANRVVALQASGNNTIVAIAAAPGAAPRPLVTL
                     LNVTPASIDPARDLGLGSPAALAAAAKTAFARKTAALVAKKTR"
     gene            3302..4033
                     /locus_tag="VF453_09100"
     CDS             3302..4033
                     /locus_tag="VF453_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687851.1"
                     /translation="MKLALLPAALVALAATFAPAAQAQVADGDFSAGLAGWSTLGDAS
                     VKTADAASPTRLWLTTASVDYEDDLDAGLPAGARNASGMPAADIGLVEPFLGVATFGL
                     DDPAGPGATEGSAAAQTFMAAAGSTLRFSWDFGTRDRVDDAAFVIVDGQLFTLATSPD
                     ATLPGIDGNATHTGWADWSTTFAAGGAHTVAFGVVDRGDYDDTSTLAIADVNLSIGAV
                     PEAPVPALLAAGLALVAVGLRRRRA"
     gene            complement(4340..>4814)
                     /gene="alkB"
                     /locus_tag="VF453_09105"
     CDS             complement(4340..>4814)
                     /gene="alkB"
                     /locus_tag="VF453_09105"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HEX7687852.1"
                     /translation="SVAMTNCGELGWVSDRRGYRYDPVDPGSGRPWPALPDAFLALAR
                     DAAERAGYPGFAPDACLVNRYGPGARLSLHQDRDELDLRAPIVSVSLGLPATFLWGGL
                     DRAERTRRVPLRHGDVVVWGGPSRLRFHGVAPLKDGTHPFAGALRLNITFRKVCP"
BASE COUNT          756 a         1904 c         1572 g          582 t
ORIGIN      
        1 gccggcgacc ctcgccgcgc cggccagcag cagcgtgtag ggcatgccgg gcatgcccgc
       61 gtcctgggcg ctccaaccga cgacccggcg atagaagtcg acggcggccg gctggtcgcc
      121 ggtcatcagc tcgtaccaga cgaagcggtt tgcagcggac atggcggatc tcctttcacg
      181 cggtggaagg gcgcggccgg cggcgacggg cggcgccggc gcggccccgc ccgcggatta
      241 tggacaccgc cgcccgcgcg gcaagtgcgc aacccgaccg tggagtgggc aggcgcccac
      301 gtggtggata ccccggtacc gtagtcacgc cgcggtcacg ccccgccaag acactcccgc
      361 ggttcttccc cgaacaccgc gtcctccatc acaaccaatc cgaccaagga tcgtccatgc
      421 aacgggccac cccctcgcgc ctcccggcga ccgcacgcgc caccctcgcg ctgctggccg
      481 ccgccgcccc cgcgtccgcg tcgtccagcg gcgtcgtcat cagccaggtc tacggcggca
      541 acggcaacac ctacgcctcc gactacgtcg agctgttcaa cgccggcgcg agcgtcgtct
      601 ccctttccgg ctggtcgatc cagtacgcga gcgcgaccgg cagcggcacc ttcgcgagca
      661 acggggtcac gacgctgaac ggcacgctgc agccgggcca gcactggctc gtgcgcctgg
      721 ccacgaccac cggccccgcg atcccgacgc cagacacctc ggccaccgcg cccaacatat
      781 ccgcgtcgtc gggcaagctg gtgctcgtcg acaactcgaa cggcctgccc tgcaacggca
      841 gcagcgcctc cccctgcacg gcggtcgcgt cgcagatcgt cgacctcgtc ggctacggca
      901 gcgccaacta cttcgagggc gcggcggctc ccggcatcag ctcgacgacc gccctgttcc
      961 gcgccaacgg cggctgcacc gacaccggcg acaactcggc cgacttcacg gccggcgcgc
     1021 ccgctccgcg caattccgcg tccccgctgg cggcctgcgc cggcggcggc ggctcgccga
     1081 ccgcgacgcc cgcggcgatc taccagatcc agggcaacgg cgcgaagagc ccgtacaacg
     1141 gccagctggt gcagacgcag ggcgtcgtga cccgggtcaa cagcaacggc tacttcctgc
     1201 aggacctcac cggcgacaac gatccgtcga cctcggacgg cgtgttcgtc tacaccggcg
     1261 ggacacccac cgcggccgcc ggccagctgt tgcagctgac cgcgaccgtc tccgagttca
     1321 acaccggcgc ggccgccaac gcggacaccg ccgcccacac cgtcaccgag ctcacgaacc
     1381 cgagccacgt gagcgtgctg ggcaccggct acacgatcgc tccggtgctc gtgaccctgc
     1441 cgctcgcgtc cgaggacgac atggagcgct acgagggcat gctcgtcacc ctgcgcggcc
     1501 cgctgacggt gcagcagaac tacttcctcg gccgcttcgg cgagctgacg ctggccaccg
     1561 gcggccgcct gcagaccccc accaacgtcc atcgtccggg tcccgacgcc aatgcgctga
     1621 acgccgacaa cctccgtcgc tcgatcctcc tggacgacgg caacaacgcg caggatccgg
     1681 acccgacgcc ctacatcggc gcggacgaca ccgtgcgcgc gggcgacacc gtcgcccagg
     1741 tcacgggcgt gatcgactac gggctgacga ccgcgaccaa caccgatccg ggcctctaca
     1801 agatccatcc gacccagccg gtggcgttca cgcgcgcgaa cccgcgcacg gccgcggccg
     1861 acgacgtggg cggcaacgtc cgcgtcgcca gcgccaacgt cgagaacttc ttcaccacgc
     1921 tggacgacgg caggaccagc tgcccgccga gcaacacgcc caacgactgc cgcggggcca
     1981 acgacgccaa cgagttctcg cgccagcgcg cgaaggtcgt cgacgagctg gccggcctga
     2041 acgccgacgt cgtcgcgctg atggagctgc agaacaacgg cgccaccgcg atccagagcc
     2101 tggtcgacgg cctcaacgcc aagctcggcg cgccggtcta tgcgcgcgtg cccgacgcgg
     2161 ccaccggcgg cggctccgac gccatcaagg tcggcatgat ctacaagccg tccacgctcg
     2221 cgctcgccgg cccggcgctg agcgacaccg ccgcgatcaa caaccgcgcc ccgatggccc
     2281 agaccttccg cctgcccaac ggcgagaagt tcaacgtgat cgccaaccac ctgaagtcca
     2341 agagctgttc cgacttcacg cccggctccg gcgacgccga ctccggcgac ctgcaaggct
     2401 gcttcaatgc gcgccgggtg cagcaggccg acgagctgcg cggcttcgtc aacacggtgc
     2461 aggccgccag cggcaccgcc gacacgctgc tcgtgggcga cttcaacgcc tacgcgaagg
     2521 aggatccggt cgtcgagctg accagcaacg gcttcgtcga cgtgatcggc cgctggatcg
     2581 ccaacggcta ctcgtacgtg ttcaacggca cctcgggccg cctggaccag gcgctcgcca
     2641 cgcccacgct ggcggccaag accaccggcg cgaccgagtg gcacatcaac gccgacgagc
     2701 cggagatcat cgactacaac gaggagttca agaagcccgc gtgcgccacc tgcggccgcg
     2761 acctctacag cgactcgccg taccgcgcct ccgaccacga cccgctcgtc gtcggcctga
     2821 acatcgtcca cgcgatcagc ggcaccgccg gtcgcgacac gatcgtcggc acgccgggcg
     2881 acgacgtcat cacgggcggg gccggcgccg acacgctgac cggcaacggc ggtcgcgacg
     2941 tcttcgtgta cgcctcgatg cgcgacgccg ccgacacgat cacggacttc cagccgggcg
     3001 acgaccgcct cgacctcgcg cagttgctcg cgagcatcgg cgcgaacccg gcgaccgcgc
     3061 tggccaaccg cgtcgtggcg ctgcaggcct ccggcaacaa cacgatcgtc gcgatcgcgg
     3121 ccgcccccgg cgccgcgccg cgaccgctcg tgacgctgct gaacgtgacg cccgccagca
     3181 tcgatccggc gcgcgacctc ggcctgggct cgcccgccgc gctcgccgct gccgcgaaga
     3241 ccgccttcgc caggaagacc gccgcgctcg tggcgaagaa gacccgctga aagacatcga
     3301 catgaaactc gcactgctcc ccgccgcgct cgtcgcgctg gccgccacct tcgcccccgc
     3361 cgcgcaggcg caggtcgccg acggcgactt ctccgccggc ctggccggct ggtcgacgct
     3421 gggcgacgcc tccgtcaaga cggccgatgc cgcgtcgccc acgcgcctgt ggctgaccac
     3481 cgcgtcggtc gactacgagg acgacctcga cgccggcctg cccgccggcg cgcgcaacgc
     3541 ttcgggcatg cccgccgccg acatcgggct ggtcgagccc ttcctcggcg tcgccacctt
     3601 cgggctcgat gatcccgcgg gcccgggcgc caccgagggc tccgccgccg cgcagacctt
     3661 catggccgcc gccggctcca cgctgcgctt cagctgggac ttcggcacgc gggatcgcgt
     3721 cgacgacgcg gccttcgtga tcgtcgacgg gcagctgttc acgctcgcga cctcgcccga
     3781 cgcgacgctg cccggcatcg acggcaacgc gacgcacacc ggctgggccg actggtcgac
     3841 caccttcgcc gccggcggcg cgcacacggt cgcgttcggc gtcgtcgacc gcggcgacta
     3901 cgacgacacc tcgacgctcg cgatcgccga cgtgaatctg agtatcggcg ccgtgcccga
     3961 ggcgcccgtt ccggcgctgc tggccgccgg cctcgcgctg gtcgccgtcg ggctgcgccg
     4021 ccgccgggcg tgacgacgga cgccggcgcg gagccctcgc cggcgtccat gtcaatccct
     4081 cgagcacgcc aacgcggtca ttcgcttgtc cacaccgtct ggggaaaagc ctgtggaaaa
     4141 cccggggagc ccgacgccgg agccgccaaa atgcggcctt cgcgttggat tgcccgcgga
     4201 cgaggcaacc gccccggttt gatgcaatcg gttacggcag cgtttcgccc cggcgtcgcg
     4261 gcgccgcgcg tgcgttcgtg acgcatcgca gcatccggtc cgtaactaca gccgacgacg
     4321 cggtgcccgc ccgccgtgct cagggacaca ccttgcgaaa ggtgatattg agccgcagcg
     4381 cgccggcgaa gggatgggtg ccatccttca acggcgcgac gccatggaag cgcagccgcg
     4441 acggcccgcc ccagacgacg acgtcgccgt ggcgcagcgg cacgcgccgc gtgcgttccg
     4501 cgcgatcgag cccgccccag agaaaggtcg ccggcaggcc gagcgagacc gagacgatgg
     4561 gcgcgcgcag gtcgagctcg tcgcgatcct ggtgcagcga cagccgcgcc ccgggcccgt
     4621 agcggttgac gaggcaggcg tcgggcgcga agccgggata gccggcgcgt tccgccgcgt
     4681 cccgggcgag ggccaggaag gcgtcgggca gtgccggcca cgggcggccc gagcccgggt
     4741 cgaccgggtc gtagcgatag ccgcggcgat cggacaccca gcccagctcg ccgcaattgg
     4801 tcatcgccac cgac
//
LOCUS       DASVLN010000373         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_504044, whole genome shotgun sequence.
ACCESSION   DASVLN010000373 DASVLN010000000
VERSION     DASVLN010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504044"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2554
                     /locus_tag="VF453_09110"
     CDS             <1..2554
                     /locus_tag="VF453_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7687853.1"
                     /translation="AKAASAAQATPGEPVAVDSVIVTANRRIETLQSVSGVVQTIDAE
                     QLRKDGINEIRSLQTEIPGMNIANQEGNIEIYIRGVGSSNNTELGDPAAASHINGIYI
                     PRPRGLGTMFYDLERVEVNKGPQGTLYGRNAMAGTLNIITAKPRLDRFGGYVQFEGGD
                     RSSGTEAAVNIPVSQTFALRAAGFVTDKTSGFHNASPDPASQALVPAGEEHNQGARLS
                     ALLVPTDRLTVSMMADYGHEGGTGYPGANIWGAVKATGLRADQLPLRDIVYRGAQGTM
                     HNDLSGLQGKVSYDFDDWGAELSLSRRTVDFYQRNAESDGIAWPGRDYSAEQWDNFST
                     QYWQTKSISKVGELRLYSAEDAKKFQWSVGAFAFNEDQKVGYLALSDGGYCCFSGTEF
                     TMPKVLGKSAALYGDGTLAVASNLRLIGGLRETEESKSRYGIGGNLALTLGAANSACC
                     ISTRIGTDGFAPNLLARPNFDMSKVQTPQQMAQFLIQTTMTPGAQDTMVSQLAPIADG
                     TNPMGACVARPDISNGLSCPTNENGGFSYANLTVPQQQVGSSKFHYTDWRLGTEYDLT
                     KDNMLYVKMSTGHKAGGFNDSFNGSTVPELFRPEQLHVLEAGSRNAFNLGGRRAVFNL
                     TGFYYDYKDQVFQDLTCITVDNTQDPPKCSGYSLVNRNVAKSHVGGLEAEGRFALPLG
                     FKFDLNGALLRTRIDSGVVADVRAIDYGKGGLSPLIDLTGNQLPLASKVNLSARLQHV
                     FSLFGGKADWQALLNWRSSYYLTQFNERPVTDNNGKVQSALAAGFPDRQEGFLTLNIG
                     AGYTFTNGFRLEAYVNNATDKQVSQKALVGADLDIRFLNDARSYGARARMDF"
BASE COUNT          485 a         1001 c          920 g          388 t
ORIGIN      
        1 ggcgaaggcc gcgtccgcgg cgcaggcgac gcccggcgag cccgtcgcgg tcgacagcgt
       61 gatcgtcacg gcaaaccgtc gcatcgagac gctgcagagc gtctcgggcg tggtgcagac
      121 gatcgacgcc gagcagctgc gcaaggacgg catcaacgag atccgctcgc tgcagaccga
      181 gatcccgggg atgaacatcg ccaaccagga aggcaacatc gagatctaca tccgcggcgt
      241 cggctcgtcc aacaacacgg agctgggcga cccggccgcg gcatcgcaca tcaacggcat
      301 ctacatcccg cgcccgcggg gcctgggaac gatgttctac gacctcgagc gcgtcgaggt
      361 gaacaagggc ccgcagggca cgctgtacgg ccgcaacgcg atggccggca cgctcaacat
      421 catcaccgcc aagccgcgcc tggaccgttt cggcggctac gtccagttcg agggtggcga
      481 ccgcagcagc ggcaccgagg cggcggtcaa catcccggtg tcgcagacct tcgcgctgcg
      541 tgcggcgggc ttcgtgaccg acaagacgtc cggcttccac aacgcctcgc ccgacccggc
      601 gtcgcaggcg ctcgttccgg ccggcgagga gcacaaccag ggcgcgcgcc tgtcggccct
      661 gctggtgccc acggatcgcc tgaccgtctc gatgatggcc gactacggcc acgagggcgg
      721 caccggctac cccggcgcca acatctgggg cgcggtgaaa gcgaccggcc tacgggccga
      781 ccagctgccg ctgcgcgaca tcgtctaccg cggcgcccag ggcacgatgc acaacgacct
      841 gtcgggcctg cagggcaagg tctcctacga cttcgacgac tggggcgccg agctgtcgct
      901 gagccggcgc acggtcgact tctaccagcg caacgccgag tcagacggca tcgcctggcc
      961 cggccgcgac tactccgccg agcagtggga caacttctcg acgcagtact ggcagaccaa
     1021 gtcgatcagc aaggtaggcg aactgcgcct gtactccgcc gaggacgcga agaagttcca
     1081 gtggagcgtg ggcgccttcg cgttcaacga ggaccagaag gtcggctacc tggcgctgtc
     1141 ggacggcggc tactgctgct tctcgggcac cgagttcacg atgcccaagg tgctgggcaa
     1201 gtcggcggcg ctgtacggcg acggtacgct ggccgtggcg tcgaacctgc ggctgatcgg
     1261 cgggctgcgc gagaccgagg agtcgaagtc gcgctacggc atcggcggca acctcgcgct
     1321 cacgctcggc gcggcgaact ccgcctgctg catctccacg cgcatcggca ccgacggctt
     1381 cgcgcccaac ctgctcgcgc gcccgaactt cgacatgagc aaggtgcaga cgccgcagca
     1441 gatggcgcag ttcctcatcc agacgacgat gacgccgggc gcgcaggaca cgatggtctc
     1501 gcagctggcg ccgatcgcgg acggcacgaa tccgatgggc gcctgcgtgg cgcggccgga
     1561 catctccaac ggcctcagct gccccaccaa cgagaacggc ggcttcagct acgccaacct
     1621 cacggtgccg cagcagcagg tcggcagcag caagttccac tacaccgact ggcgcctggg
     1681 cacggagtat gacctgacga aggacaacat gctctacgtc aagatgtcga ccggccacaa
     1741 ggcgggcggc ttcaacgaca gcttcaacgg ctcgacggtg cccgagctgt tccgccccga
     1801 gcaactgcac gtgctggagg ccggctcgcg caacgcgttc aacctgggcg gccggcgcgc
     1861 ggtcttcaac ctgacgggct tctactacga ctacaaggac caggttttcc aggacctgac
     1921 ctgtatcacg gtggacaaca cgcaggatcc gccgaagtgc agcggctact cgctggtcaa
     1981 ccgcaacgtc gcgaagtcgc acgtcggcgg cctcgaggcc gagggccggt tcgcgctgcc
     2041 cctgggcttc aagttcgacc tcaacggcgc gctgctgcgc acgcgcatcg actcgggcgt
     2101 cgtcgccgat gtgcgcgcga tcgactacgg caagggcggc ctgagcccgc tgatcgacct
     2161 gaccggcaac cagctgcccc tggcttccaa ggtcaacctc tcggcgcggc tgcagcacgt
     2221 cttcagcctg ttcgggggca aggccgactg gcaggcgctg ctgaactggc gctcgtcgta
     2281 ctacctcacg cagttcaacg agaggccggt gacggacaac aacggcaagg tgcagagcgc
     2341 gctggccgcc ggcttccctg accgccagga gggcttcctc acgctgaaca tcggcgctgg
     2401 ctacaccttc accaacggct tccggctcga ggcctacgtg aacaacgcca ccgacaagca
     2461 ggtgtcgcag aaggcgctgg tcggcgccga cctggacatc cgcttcctga acgacgcgcg
     2521 atcctacggc gcgcgcgccc gcatggactt ctgagtacgc tacgcgtcgc gcccgatgcc
     2581 cggcgcgcca ggcccgcagc gcttgcaagg cgcctgcggg ccgcttcact tccgaaggga
     2641 ccctcccatg atcacgatgc gcccgctcgg cgccgccctg gccgccggcc tgctgggact
     2701 gctggccgcc tgcgcgtccg cgccgccgtc cgcgccgtcg ccgcagcaag cgttgccgac
     2761 cgcgacttcc gacgcgctgg cggacttccc gccg
//
LOCUS       DASVLN010000374         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_394886, whole genome shotgun sequence.
ACCESSION   DASVLN010000374 DASVLN010000000
VERSION     DASVLN010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394886"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VF453_09115"
     CDS             <1..1030
                     /locus_tag="VF453_09115"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7687854.1"
                     /translation="AARALRAIADTRGEAPYTGEVAQALVAFARAQGGALAAEDLAAY
                     RPQWVETISRAYRGYTLHEIPPNGQGIAALIALGILERFDLASMAPDSPECHHLQIEA
                     MKLAFADVYRHCAELEAMTVTPAQLLDDAYLAERAKLVDPARAQAFAHGNPVRGGTIY
                     LAAADERGMMVSFIQSNYMGFGSGCVEPTFGISLQNRGHGFSLDPSSPNVVAPRKRPF
                     HTIIPAFLMKDGAPAMSFGVMGGNMQPQGHVQTLVRMLDHRQGPQAACDAPRWRFNAG
                     RNLNVETAMPEATVAGLAALGHEIDRLDDSYQDFGAGQFIWRLGDPAAEGYVAASDPR
                     RDGQAAGY"
     gene            1110..1595
                     /locus_tag="VF453_09120"
     CDS             1110..1595
                     /locus_tag="VF453_09120"
                     /inference="COORDINATES: protein motif:HMM:NF042776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6714 family protein"
                     /protein_id="HEX7687855.1"
                     /translation="MTSDDPFGALVADLFTAFAADGTAVPALTLRGGDELDAGRPAPP
                     FDVLVDTISDTYLEAHAWGSGWLDAASWRHVLPFMLAYALRHLDAPSEVTDAALTSLR
                     PPDRDPPRLATLTPAQETVVLRVLDVLAHAPGAATADLAAQVLSEWWTPGAIPRAADE
                     D"
     gene            complement(1635..1991)
                     /locus_tag="VF453_09125"
     CDS             complement(1635..1991)
                     /locus_tag="VF453_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687856.1"
                     /translation="MLLRLVPKIFYADRAVGIDLFVDGLRFSVDHEDDTITVVSRDGC
                     KAILVQDAEFAAKDRPEIAIETDAIDEIHADVASRRPDLLHPNPVVHAPTQRPWGARE
                     FAVIDRSGVCVVFRQW"
     gene            complement(2032..>2672)
                     /locus_tag="VF453_09130"
     CDS             complement(2032..>2672)
                     /locus_tag="VF453_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7687857.1"
                     /translation="DAPAAEPALPPEVPVGLPGDLLRRRPDVAKSEADLHAATAKVGQ
                     AKAQLFPSITLGAFGGLQASHAGDLFDWASRFWAGGAQVSIPIFEGGKLKAQIRTADA
                     QMKEAALQYRGTVLSAYHDADDAITAYAHEQRHAAALARQLADARRSRDLMRDRWKNG
                     FVSLIEVLDAQRQLHQSELQALQSRVAASTDLVALYKALGGDWDPATRSAQR"
BASE COUNT          352 a         1006 c          962 g          352 t
ORIGIN      
        1 cgcggcgcgg gcgctgaggg cgatcgcgga cacgcgcggc gaggcgccgt acacgggcga
       61 ggtcgcgcag gcgctggtcg ccttcgcgcg cgcgcagggc ggggcgctcg cggccgagga
      121 tctcgccgcg taccggccgc aatgggtcga gacgatctcg cgcgcctacc gcggctacac
      181 gctgcacgag atcccgccca acggccaggg catcgcggcg ctcatcgcgc tgggcatcct
      241 cgagcgcttc gacctcgcgt cgatggcgcc cgactcgccg gagtgccacc acctgcagat
      301 cgaggcgatg aagctcgcgt tcgcggacgt ctatcgccac tgcgcggagc tcgaggccat
      361 gacggtgacg ccggcgcagc tcctggacga cgcctacctg gccgagcgcg cgaagctcgt
      421 cgacccggcg cgggcgcagg ccttcgcgca cggcaacccc gtccggggcg gcacgatcta
      481 cctcgccgcg gccgacgagc gcggaatgat ggtcagcttc atccagagca actacatggg
      541 cttcggctcc ggctgcgtgg agccgacctt cggcatcagc ctgcagaacc gcggccacgg
      601 cttcagcctc gacccgtcga gccccaacgt ggtcgcgccg cgcaagcggc cgttccacac
      661 catcatcccg gccttcctca tgaaggacgg cgcgcccgcg atgagcttcg gcgtgatggg
      721 cggcaacatg cagccgcagg gccacgtgca gacgctggtt cgcatgctcg accatcgcca
      781 gggcccgcag gcggcctgcg acgcgccgcg ctggcgcttc aacgcggggc gcaacctgaa
      841 cgtcgagacc gcgatgcccg aggccacggt cgcgggcctg gccgccctcg gccacgagat
      901 cgaccgcctc gacgacagct accaggactt cggtgccggc cagttcatct ggcgcctggg
      961 cgacccggcc gccgagggct acgtggcggc cagcgacccc cgccgcgatg gccaggcggc
     1021 aggctactga gcggaccgcc gcgcgacccg cccctcgggc cgggggcgga ggaacggcgg
     1081 tgcgcgagcc cggcgcgtaa gatcccgcga tgacctccga cgatcccttc ggcgcgctcg
     1141 tcgccgacct gttcaccgcg ttcgccgccg acggcacggc cgtacccgcg ctgacgctgc
     1201 gcggcggcga cgagctcgac gccggccgtc cggcgccgcc cttcgacgtg ctcgtcgaca
     1261 cgatctccga cacctatctc gaggcccacg cgtggggctc gggctggctc gacgccgcgt
     1321 cctggcgcca cgtcctgccc ttcatgctcg cctacgcgct gcgccacctc gacgcgccca
     1381 gcgaggtcac cgacgccgcg ctcacctcgc tgcgcccgcc cgaccgcgac ccgccgcgcc
     1441 tggccacgct caccccggcc caggagacgg tcgtcctgcg cgtgctggac gtcctcgccc
     1501 acgcccccgg cgccgcgacg gccgacctcg ccgcgcaggt gctgtcggag tggtggacgc
     1561 cgggcgcgat cccgcgagcg gccgacgagg actgacgccg tccgggcggc cggggacgtc
     1621 gcttgccctg gccttcacca ctgccggaag acgacgcaga ccccgctgcg gtcgatcacc
     1681 gcgaactcgc gcgcacccca gggccgctgc gtcggcgcgt gcacgacggg gttcgggtgc
     1741 aacaggtcgg ggcggcggga ggcgacgtcg gcgtggatct cgtcgatcgc gtcggtctcg
     1801 atcgcgatct cgggtcggtc cttcgcagcg aactcggcgt cctgcacgag gatcgccttg
     1861 cagccgtcgc ggctcacgac cgtgatcgtg tcgtcctcgt ggtcgaccga gaagcgcagg
     1921 ccgtcgacga acaggtcgat gccgacggcg cggtcggcgt agaagatctt ggggacgagg
     1981 cggagcagca tcgtggcgcc cttcggggat ggaagggcgc agcatgccac gtcagcgctg
     2041 cgccgacctc gtcgccggat cccagtcgcc gcccagcgcc ttgtacagcg ccacgagatc
     2101 ggtcgacgcc gcgacgcggc tctgcagcgc ctgcagctcc gattggtgga gctggcgctg
     2161 cgcgtccagc acctcgatca gcgagacgaa gccgttcttc cagcgatccc gcatgaggtc
     2221 gcggctgcgg cgggcgtcgg cgagctggcg cgcgagcgcg gcggcgtggc gctgctcgtg
     2281 cgcgtaggcg gtgatcgcgt cgtcggcgtc gtggtaggcc gagagcaccg tgccgcggta
     2341 ctgcagcgcc gcttccttca tctgcgcgtc ggcggtgcgg atctgcgcct tgagcttgcc
     2401 gccctcgaag atcgggatcg acacctgcgc gccgccggcc cagaagcgcg acgcccagtc
     2461 gaacaggtcg cccgcatgcg atgcctgcag cccgccgaag gcgcccagcg tgatcgacgg
     2521 gaacagctgc gccttcgcct ggccgacctt ggcggtcgcg gcgtgcaggt cggcctcgct
     2581 cttggcgacg tcgggccggc ggcgcagcag gtcgccgggc aggccgaccg gcacttcggg
     2641 cggcagcgcc ggctcggcgg cgggcgcgtc ac
//
LOCUS       DASVLN010000375         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_181011, whole genome shotgun sequence.
ACCESSION   DASVLN010000375 DASVLN010000000
VERSION     DASVLN010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181011"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VF453_09135"
     CDS             complement(<1..1007)
                     /locus_tag="VF453_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7687858.1"
                     /translation="MSKRAGAPPTIVDVAKKAGVSIKTVSRVMNKESTVHPDTRALVL
                     QVVNELNYRPQLSARSLAGARSFIIGLLYYEPSAAYVAGVQGGATLRCRESGRHLVVE
                     SLRSDDADLAGRIQAMLSALRPDGMILTPPLCDHPQILAALREAGTPTVLISPGSGGH
                     GLAQVGMDDTLAAEEVTNLLLSLGHRHIAFVQGDQVASERRQRGFERALRAHGLAPDP
                     ALVLPGRFDFDSGVAAAHALLSRSAPPTAVFAANDDMALGVFAAAHRLGIAVPRELSV
                     VGFDDSRAATLVWPPLTTVRQPLAEMATEAVDLLLSEEMKPGAAGPPPARLLPHEVVV
                     RG"
     gene            1267..1731
                     /locus_tag="VF453_09140"
     CDS             1267..1731
                     /locus_tag="VF453_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX7687859.1"
                     /translation="MKTWLKRTLIGVAACAAVAGSLAAYSQAPADPAVREAHMLEHVG
                     KRLNLDANQSARLKALADLLHAQHAAGGDLHRRVQALFAGNTFDRAGAQQLVSEKVAQ
                     VQASGPAVINAVGDFYDSLNATQQQQVRDFMARAHHHGMWMHHHGASAPAGN"
     gene            1980..2654
                     /locus_tag="VF453_09145"
     CDS             1980..2654
                     /locus_tag="VF453_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7687860.1"
                     /translation="MRRILMIDDDALLAAPLATYFKRFDFELDSATRPSEGLARLRAQ
                     PYDAAILDVMLPEMDGFELCRTIRRESDIPIVMLTARGEVMDRIVGLEIGADDYIPKP
                     FEPRELLTRIQTVLRRARGPAAAAQKLVFDGLEIDLERRQVLRQGELVELTGTEFELL
                     AMLARSPQKVFHRDEILNRLRGQDAELYTRAVDILVSRLRRKLEPLDCIKTLRNAGYA
                     FAGGHA"
     gene            2647..>3017
                     /locus_tag="VF453_09150"
     CDS             2647..>3017
                     /locus_tag="VF453_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687861.1"
                     /translation="MPERADARGRRLLFPSLGARLVGLFLVLAVAVAITFLIGRNMMQ
                     HLGWREYLRPLVANYTDMLVSEIGSPPDIERARLLAQRLPLHIRIDGPDIDWDSADEP
                     PPSAPAARPGPRHPVVTLPDGA"
BASE COUNT          434 a         1128 c         1068 g          387 t
ORIGIN      
        1 ccgcgcacca ccacctcgtg cggcagcagg cgcgccggcg gcgggccggc ggcgccgggc
       61 ttcatctcct cggacagcag caggtcgacg gcctccgtgg ccatctcggc cagcggctgg
      121 cgcacggtgg tgagcggcgg ccagaccagc gtcgcggcgc gcgaatcgtc gaagccgacg
      181 accgagagct cgcgcggcac cgcgatgccg agccggtgcg cggccgcgaa cacgccgagc
      241 gccatgtcgt cgttggcggc gaagaccgcg gtcggcggtg cggagcggct gaggagcgcg
      301 tgcgccgcgg cgacgccgga gtcgaaatcg aagcggcccg gcagcacgag cgccggatcg
      361 ggcgcgaggc catgggcccg cagcgcacgc tcgaagccgc gctggcgccg ctcgctggcg
      421 acctggtcgc cctggacgaa ggcgatgtgg cgatggccca ggctcagcag caggttcgtg
      481 acctcctcgg ccgcgagcgt gtcgtccatg ccgacctgcg ccagcccgtg gccgccgctg
      541 ccgggcgaga tcagcaccgt cggcgtgccg gcctcgcgca gcgccgcgag gatctgcggg
      601 tggtcgcaca gcggcggggt gaggatcatg ccgtccggcc gcagcgccga cagcatcgcc
      661 tggatgcggc cggcgaggtc ggcgtcgtcg ctgcgcaggg actcgacgac caggtgccgt
      721 cccgactcgc ggcagcgcag cgtcgcgccg ccctggaccc ccgcgacgta ggcggcgctc
      781 ggctcgtagt acagcaggcc gatgatgaac gagcgcgcgc cggccaggct gcgcgccgac
      841 aactgcggcc ggtagttcag ctcgttcacg acctgcagca ccagggcgcg cgtgtcgggg
      901 tggaccgtgc tctccttgtt catgacccgc gacacggtct tgatcgacac gcctgccttc
      961 ttcgcgacgt cgacgatggt gggcggtgcg cccgcgcgct tgctcaagtg cggccctctc
     1021 tcttgtgcgt tcgtgttcga ggcgtcggcg ccgcctgctg tcgcgccgcc ggacgggccc
     1081 caatgtgcca caagcgcggc ggcaaggtcg cggacagctc cgtgcggtcg ccggaatcct
     1141 cgcctcgggc gacccgctac aaaacgaaac acggcgatac ggcagcgaaa gcatctcgcg
     1201 gacgcgatgg cgcgtaatga gcccgtgccg ctccaccgac cggcgccacc caacgaagga
     1261 atcaccatga agacctggct caagagaacc ctgatcggcg tcgccgcctg cgccgcggtc
     1321 gccggcagcc tcgcggccta ttcgcaagcg cccgccgacc cggcggtccg cgaggcgcac
     1381 atgctcgagc atgtcggcaa gcgcctgaac ctcgacgcca accagtcggc caggctcaag
     1441 gcgctggccg acctgctgca cgcccagcac gcggcgggcg gcgacctgca ccggcgcgtc
     1501 caggcgctgt tcgcgggcaa caccttcgac cgcgccggcg cgcagcagct ggtcagcgag
     1561 aaggtcgccc aggtgcaggc gagcggcccc gcggtgatca acgccgtcgg cgacttctac
     1621 gacagcctga acgccacgca gcagcaacag gtgcgcgact tcatggcccg cgcccaccac
     1681 cacggcatgt ggatgcacca ccacggcgcc tcggcgccgg cgggcaactg agcgcgtctg
     1741 cacgcgctgc ataatcgcgt gcgcgtcccg cgaagcgcgc tgcgggccct ggcgctgccg
     1801 ctcgcccgcg tccaacgagg cacgggcaag cggcggatcc ggctccgccg gcgccgcacg
     1861 cctgccgggg ccgcaagcgg cctggcgttc gccaggcgac aggccgtcca ctgcacggcc
     1921 tgtgtccggg ctcgccccct cggggacggg cagcgccagc gaccgtggag gtcaacaatt
     1981 tgcgtcggat attgatgatc gacgacgacg cgctcctcgc cgcgccgctc gccacctact
     2041 tcaagcgctt cgacttcgag ctcgacagcg ccacgcgccc cagcgagggc ctcgcccgcc
     2101 tgcgcgcgca gccctacgac gccgccatcc tcgacgtgat gctgccggag atggacggct
     2161 tcgagctgtg ccgcacgatc cggcgcgaga gcgacatccc catcgtcatg ctgaccgccc
     2221 gcggcgaggt gatggatcgc atcgtcggcc tggagatcgg cgccgacgac tacatcccca
     2281 agccgttcga gccgcgcgag ctgctcacgc gcatccagac cgtgctgcgg cgggcgcgcg
     2341 ggccggcggc cgctgcgcag aagctggtgt tcgacggact cgagatcgac ctcgagcgcc
     2401 gccaggtgct gcgccagggc gagctggtcg agctcaccgg cacggagttc gagctgctcg
     2461 cgatgctcgc gcgctcgccg cagaaggtct tccaccgcga cgagatcctc aaccggctgc
     2521 gcgggcagga cgccgagctc tacacgcgcg cggtcgacat cctcgtgagc cgcctgcgca
     2581 gaaagctcga gccgctggac tgcatcaaga cgctgcgcaa cgccggctac gcgttcgcgg
     2641 ggggacatgc ctgaacgcgc cgacgcgcgc ggccggcggc tgctgttccc gtcgctcggg
     2701 gcgcggctgg tcggcctgtt cctcgtgctc gcggtggcgg tggcgatcac cttcctgatc
     2761 ggccgcaaca tgatgcagca cctgggctgg cgcgagtacc tgcggccgct cgtggccaac
     2821 tacaccgaca tgctggtctc ggaaatcggc tcgccgccgg acatcgagcg cgcgcgcctg
     2881 ctcgcccagc gcctgccgct gcacatccgc atcgacgggc cggacatcga ctgggactcc
     2941 gccgacgaac cgccgccttc cgcgccggcc gcgcgcccgg ggccgcggca cccggtcgtc
     3001 acgctgccgg acggcgc
//
LOCUS       DASVLN010000376        10728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_394983, whole genome shotgun sequence.
ACCESSION   DASVLN010000376 DASVLN010000000
VERSION     DASVLN010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10728
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394983"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VF453_09155"
     CDS             complement(<1..392)
                     /locus_tag="VF453_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEX7687862.1"
                     /translation="MTRLAVVGALHEEIAAIQPCLAGVRVERRAGRDFLIGRLDGHDV
                     VLVRCGIGKVAAATTASMLLDAFDASALLFTGVAGGIGASVRTGDIVVARTLLQHDMD
                     ASPLFPRFEVPLTGRARFDADPAWSARLA"
     gene            complement(450..938)
                     /locus_tag="VF453_09160"
     CDS             complement(450..938)
                     /locus_tag="VF453_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687863.1"
                     /translation="MMMTMTRADTYVRTEAGKAEIRNRSLQLSRPARNLLMVIDASCT
                     GEAWIAKVLGSTRADLDQLLSAGLVAVATDAPRPSGPQRVTLAQAIREWSYDALYTLL
                     THEARERFGLIKGYRLILKIEGCANLEGLQSVALEFAEQIRKVHGEEPALRFRRQLGA
                     TE"
     gene            complement(978..2657)
                     /locus_tag="VF453_09165"
     CDS             complement(978..2657)
                     /locus_tag="VF453_09165"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX7687864.1"
                     /translation="MERNWLKNYPAGVAQHVDASEYASLVELLDNSFKKYAERPAFKF
                     MGLDFSYARIDELSRRFAAYLQTLGLAQGDRVALMMPNIPQYPVAVAAVLRAGLVVVN
                     VNPLYTPRELEHQLKDSGAKAIVVFEKFATTLQQVMHEVPTKKVVLASIGDMLGGLRG
                     LVTNFAYRTIKKEVPAFRIPGAVRFNDALAQGLRATFRPVKVGPNDVAVLQYTGGTTG
                     VSKGAVLLHRNLVANVLQSHAWYQPALAKAKAGEQILTICALPLYHIFGFNTNMMLGM
                     RIGGCNLLVANPRDIPSMLKDLRRQPFHSLPAVNTLFNALANHPDFGTVDWSSLVLSV
                     GGGMAVQQATAQLWLEKTGCSICEGYGLSETSPAATCNPVESKAYSGTIGMPMPDTDV
                     VLLDDDGNEVGPGERGEIAIRGPQVMAGYWQRPDETAKVMTADGFFRTGDIGEVDERG
                     YFKIVDRKKDMILVSGFNVYPNEVEDVVALLPGVLECAAVGVPDDKTGEAVKVVIVRK
                     DAAVDEAKVKAWCETNLTGYKRPRFIEFRTELPKTPVGKILRRELRDRKPA"
     gene            complement(2769..4193)
                     /locus_tag="VF453_09170"
     CDS             complement(2769..4193)
                     /locus_tag="VF453_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX7687865.1"
                     /translation="MTLNLVTLLYLVASVCFIQALKGLSHPTTSIRGNVFGMTGMAIA
                     VFTTAALIATLAHSPLGLGWVVLGLLIGGTAGTVMARRVEMTKMPELVAFMHSMIGLA
                     AVFIAVAAVAEPWALERGLARGEAIPVGNRLELFLGAAIGAITFSGSVIAFGKLSGKY
                     KFRLFQGAPVTFSGQHLLNLVLGIVTIGLGATFVATENAAAFGLMLALAFVLGVLIII
                     PIGGADMPVVVSMLNSYSGWAAAGIGFSLDNAMLIIAGSLVGSSGAILSYIMCKAMNR
                     SFFNVILGGFGAEPGTAATGAAQQRPVKSGSADDAAFILGNAESVIIVPGYGLAVARA
                     QHAVKELAAKLSSKGVSVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMDEINGEFG
                     QADVAIVLGANDVVNPAALTKGSPIYGMPILEAYKARTVIVNKRSMAAGYAGLDNELF
                     YMDKTMMVFGDAKKVVEDMLKAVE"
     gene            complement(4255..4503)
                     /locus_tag="VF453_09175"
     CDS             complement(4255..4503)
                     /locus_tag="VF453_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687866.1"
                     /translation="MTDLRGDERPPPGRGPFWRVVRLVAAVALFLYALRQLGPQPIGR
                     LPLALIAGLLLAGAMGVTDSVVGLLAAAIEKLHKDRQA"
     gene            complement(4500..4814)
                     /locus_tag="VF453_09180"
     CDS             complement(4500..4814)
                     /locus_tag="VF453_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX7687867.1"
                     /translation="MDIVSPTIVNLIIFVLAIYVGFHVVWNVTPALHTPLMAVTNAIS
                     AIVIVGAMLAASMTEGWFAKTMGVLAVALAAVNVFGGFLVTRRMLEMFRKKERPPAKD
                     AS"
     gene            complement(4833..5963)
                     /locus_tag="VF453_09185"
     CDS             complement(4833..5963)
                     /locus_tag="VF453_09185"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX7687868.1"
                     /translation="MLIGVPLETAAGETRVAVTPETAKKLAAAGHAVRVQSGAGLAAS
                     ATDEAYVAAGASIVDRAAALGAELVLKVRAPQADELGLMKPAAVVVGMLNPFDREGLQ
                     RLAAARLTAFALEAAPRTTRAQSMDVLSSQANIAGYKAVMIAADKYQRLFPMLMTAAG
                     TIKAARVVVLGVGVAGLQAIATAKRLGAVIEASDVRPSVKEQVESLGAKFIDVPYETA
                     EEKEAAEGVGGYARPMPQSWLDRQKLEVAKRVAQADVVITTALIPGRAAPVLVTEDMV
                     RAMKPGSVVVDLAAGRGPAGSDGNCPLTEAGRTVIRHGVTIVGETNLPALVAADASSL
                     YARNVLDFLKLVTTKEAGFKLDLEDDIVAACLVAHEGEVRRA"
     gene            6151..6690
                     /locus_tag="VF453_09190"
     CDS             6151..6690
                     /locus_tag="VF453_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7687869.1"
                     /translation="MAKERWKPSVTVAAVIEKDGRYLMIEEMTRDGLRLNNPAGGLEA
                     GESILEGAIREALEETACHFTPEALLGVYMARNPDTARGPGVTYVRFAFCGSASDPVP
                     GRALDSPIVRTLWMTADEIVAARDRLRGPLVLQCIEDHRAGRRLPLDAIHTHASVANP
                     DPAQATAEPDDPPAGAAAP"
     gene            6749..7870
                     /gene="mnmA"
                     /locus_tag="VF453_09195"
     CDS             6749..7870
                     /gene="mnmA"
                     /locus_tag="VF453_09195"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX7687870.1"
                     /translation="MSAMRVVVGLSGGVDSAVTAHLLKQQGHEVVGIFMKNWEDDDDD
                     EYCSSRQDFLDAASVADVLGIEIEHVNFAADYKDRVFAEFLREHRAGRTPNPDVLCNA
                     EIKFKAFLDHAMRLGADKIATGHYARVRLNAATGRHELLKGLDPAKDQSYFLHRLNQS
                     QLAQTMFPVGELRKTEVRRIADEIGLPNAKKKDSTGICFIGERPFREFLNRYLAHDPG
                     PILDDRGRKLGEHVGLSFYTLGQRQGLGIGGVKDKRGERGGGDHAPWFVARKELATNT
                     LRVVQGHDHPWLLSRRLTATDLAWCADLPPAPGVLAAKTRYRQADAACTLEPPAEPGS
                     ITLDFPLPQWAVTPGQSAVLYEGDVCLGGGVIDSAVTEG"
     gene            7954..8322
                     /locus_tag="VF453_09200"
     CDS             7954..8322
                     /locus_tag="VF453_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEX7687871.1"
                     /translation="MLAQVANVAVALIAALHVYILVLEMFLWTTPRGRRAFGLTPEFA
                     QQSKALAANQGLYNGFLAAGLAWGLWQGPAGVATTLFFLGCVLVAGVFGAATASRKIL
                     FIQAIPAAIAIALVLLAPSR"
     gene            8439..9386
                     /locus_tag="VF453_09205"
     CDS             8439..9386
                     /locus_tag="VF453_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEX7687872.1"
                     /translation="MNFQQLRSVREAVRRGFNLTEVAQALHTSQPGVSRQIRELEDEL
                     GIEIFVRVGKRLTGLTQPGHAVLPWVERLLQDAESLQRASEEFTQGTTGVLSIAATHS
                     QARYALPTAVRDFRAAHPNVQLQLHQGSPEQIAQMLLTGTVDIGIASHLLAERPELVA
                     LPCYRWTHSIVVPEGHPLQADGNVTLERLAQYPIITYDTGLTGRWSIDAAFKRAGLRP
                     EIVLTAMDADVIKTYVDLGLGVGILASIAIDEDFPARLRAIDARNLFSINTTSVAVRR
                     GGLLRGFAYEFIQTFASHLSRDVVEAAQRAPSDTEQAPL"
     gene            9562..>10728
                     /locus_tag="VF453_09210"
     CDS             9562..>10728
                     /locus_tag="VF453_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7687873.1"
                     /translation="MTSTQPPAARPDPGLRPDRVADPARLLEHATWLLYIDGRRAGEL
                     GVQVLEATAREPTHRLRGDAWWHVAYSKLRAAEVEAALQANEQARACYLAHDDQRGLL
                     MCDEVDGLYLRSRGRLQEALELHLRISARTDVARTPDDLYITHNSRALTRKQLGQSDA
                     MLLDFYQALAAAQTCESPGPNINALVNLGGSHGDLYNLTEAQTLSERVLDLAEEAGAW
                     NAFAVAAFNLMQACDGLGARERCADVLARLRRNEVHMPAGILRQNGPCMAIAHLCAGD
                     LEGAQAWLDLGATAPFADGDGKTDFARARACTLMALGRDAEAREVVEARIAECAHSTF
                     HDPPYTRMRLLQAATDACERLYDTAAALRYLHEVHALYEQLVGRSSRAGFIAAQA"
BASE COUNT         1570 a         3923 c         3703 g         1532 t
ORIGIN      
        1 gcgaggcggg cgctccacgc gggatcggcg tcgaagcgcg cgcggccggt cagcggcacc
       61 tcgaagcggg gaaacagcgg agaggcgtcc atgtcgtgct gcagcagcgt gcgcgcgacg
      121 acgatgtccc ccgtccgcac gctcgcgccg atgccgccgg ccacgccggt gaacagcagc
      181 gcgctcgcgt cgaacgcgtc cagcagcatc gacgccgtgg tcgcggcggc gaccttgccg
      241 atgccgcaac gcacgaggac gacgtcgtgg ccgtcgaggc ggccgatcag gaagtcgcgg
      301 ccggcgcggc gctcgacgcg cacgccggcc aggcacggct ggatcgcggc gatctcctcg
      361 tgcagcgcgc cgacgacggc caggcgcgtc aacgcgcggt ctccggcctc gccggcggcg
      421 cggatcccgg ggtcatcgcg ccgcgaccgt cactcggtcg cgcccagctg cctgcgaaag
      481 cgcagcgccg gctcctcgcc gtgcaccttg cggatctgct cggcgaattc gagggcgacg
      541 ctctgcaggc cctcgaggtt ggcgcagccc tcgatcttga ggatcagccg gtagcccttg
      601 atgaggccga agcgctcgcg ggcctcgtgc gtgagcagcg tgtacagcgc gtcgtacgac
      661 cattcccgga tggcctgcgc cagggtcacg cgctgcgggc cggagggacg cggcgcgtcg
      721 gtcgcgaccg cgacgagccc cgcggagagc agctggtcga ggtcggcgcg cgtggagccg
      781 agcaccttgg cgatccacgc ctcgccggta caggaggcgt cgatgaccat gagcaggttg
      841 cgcgccgggc gcgacagctg caggctgcga tttcggatct cggccttgcc ggcctcggtg
      901 cggacgtagg tgtccgcgcg cgtcatcgtc atcatcatgg ttccaacacg tcagcgccgg
      961 caggaggcgc acgcccgtca ggcgggcttt ctgtcccgca gctcgcgccg caggatcttt
     1021 cctaccgggg tcttcggcaa ttctgtccgg aactcaatga agcgcggccg cttgtaacct
     1081 gtaagatttg tttcacacca ggccttgacc ttggcctcgt cgaccgccgc gtccttcctg
     1141 acgatcacca ccttgaccgc ctcgccggtc ttgtcgtcgg gcacgccgac ggcggcgcac
     1201 tccagcaccc cgggcagcag cgcgacgacg tcctcgacct cgttcgggta cacgttgaag
     1261 cccgagacga ggatcatgtc cttcttgcgg tcgacgatct tgaagtagcc gcgctcgtcg
     1321 acctcgccga tgtcgccggt gcggaagaag ccgtcggccg tcatgacctt ggccgtctcg
     1381 tccgggcgct gccagtagcc ggccatcacc tgcgggccgc ggatggcgat ctcgccgcgc
     1441 tcgcccggcc cgacctcgtt gccgtcgtcg tccagcagca cgacgtcggt gtcgggcatg
     1501 ggcatgccga tggtgccgct gtaggccttc gattcgaccg ggttgcaggt cgcggcgggc
     1561 gaggtctccg acaggccgta gccctcgcag atcgagcagc ccgtcttctc gagccacagc
     1621 tgcgccgtcg cctgctgcac cgccatgccg ccgccgacgg acagcacgag gctcgaccag
     1681 tcgaccgtgc cgaagtccgg atggttggcc agcgcgttga acagcgtgtt gaccgcgggc
     1741 aggctgtgga acggctggcg gcgcaggtcc ttcagcatcg acgggatgtc gcgcgggttg
     1801 gcgaccagca ggttgcagcc gccgatgcgc atgcccagca tcatgttcgt gttgaagccg
     1861 aagatgtggt acagcggcag cgcacagatc gtcaggatct gctcgcccgc cttggccttg
     1921 gcgagcgccg gctggtacca ggcgtgcgac tgcagcacgt tggcgaccag gttgcggtgc
     1981 agcagcacgg cgcccttgct gacgccggtg gtgccgccgg tgtactgcag cacggcgacg
     2041 tcgttcgggc cgaccttcac cggccggaag gtcgcgcgca ggccctgcgc gagcgcgtcg
     2101 ttgaagcgca ccgcgccggg gatgcggaag gcaggcactt ccttcttgat cgtgcggtag
     2161 gcgaagttgg tgaccaggcc gcgcaggccg cccagcatgt cgccgatcga cgcgagcacg
     2221 accttcttcg tcggcacctc gtgcatcacc tgctgcagcg tggtcgcgaa cttctcgaag
     2281 acgacgatcg ccttggcgcc cgagtccttg agctggtgct cgagctcgcg cggcgtgtac
     2341 agcgggttga cgttgacgac gaccaggccc gcgcgcagca ccgccgcgac ggccaccggg
     2401 tactgcggga tgttgggcat catcagcgcg acgcggtcgc cctgcgcgag gcccagcgtc
     2461 tgcaggtagg cggcgaagcg gcgcgacagc tcgtcgatgc gcgcgtagct gaagtccagg
     2521 cccatgaact tgaacgcggg ccgctcggcg tacttcttga agctgttgtc cagcagctcg
     2581 accagcgagg cgtactcgct cgcgtcgacg tgctgcgcca cgccggcggg gtagttcttc
     2641 aaccagttgc gttccatctt gtctccgggg gctcgcccgt ccaggggctg ttcgtatggc
     2701 agtgaagcga aggaagggat tctgccgcgc cacgccgcgg cgcgttcacg gccgcggcgt
     2761 cgcggcggtc attcgacggc cttgagcatg tcctcgacca ccttcttggc gtcgccgaag
     2821 accatcatcg tcttgtccat gtagaagagc tcgttgtcga gcccggcgta gccggcggcc
     2881 atggagcgct tgttgacgat caccgtgcgg gccttgtagg cctccaggat cggcatgccg
     2941 tagatcggcg agcccttggt gagggcggcc gggttcacca cgtcgttggc gccgagcacg
     3001 atggccacgt cggcctgccc gaactcgccg ttgatctcgt ccatctcgaa gacctggtcg
     3061 tagggcacct cggcctcggc cagcagcacg ttcatgtggc ccggcatgcg gccggccacc
     3121 gggtggatcg cgtacttcac gctcacgccc ttgctcgaca gcttggccgc gagctccttg
     3181 accgcgtgct gcgcgcgcgc gaccgccagg ccgtagcccg gcacgatgat cacgctctcg
     3241 gcgttgccga ggatgaaggc cgcgtcgtcg gcgctgccgc tcttgaccgg ccgctgctgc
     3301 gcggcaccgg tcgccgccgt gcccggctcg gcgccgaagc cgcccaggat cacgttgaag
     3361 aacgagcggt tcatcgcctt gcacatgatg tagctcagga tcgcgcccga gctgcccacc
     3421 agcgagcctg cgatgatcag catcgcgttg tccagcgaga agccgatgcc cgcggccgcc
     3481 cagcccgagt agctgttgag catcgacacc accaccggca tgtcggcgcc gccgatcggg
     3541 atgatgatca ggacgcccag cacgaacgcg agcgcgagca tgaggccgaa ggcggccgcg
     3601 ttctcggtgg cgacgaaggt cgcccccagg ccgatcgtca cgatcccgag caccaggttg
     3661 agcaggtgct ggccggagaa ggtcaccggc gcgccctgga acagccggaa cttgtacttg
     3721 cccgacagct tgccgaacgc gatcaccgac cccgagaacg tgatcgcgcc gatcgccgcg
     3781 cccaggaaca gctcgagccg gttgcccacc gggatcgcct cgccgcgcgc gagcccgcgc
     3841 tccagcgccc agggctcggc caccgccgcg accgcgatga agacggcggc caggccgatc
     3901 atcgagtgca tgaaggcgac gagctccggc atcttggtca tctcgacgcg ccgcgccatg
     3961 acggtgccgg ccgtcccgcc gatcagcagc cccagcacca cccagccgag ccccagcggc
     4021 gaatgcgcga gcgtggcgat cagcgccgcg gtcgtgaaga cggcgatcgc catgccggtc
     4081 atgccgaaga cgttgccgcg gatcgacgtc gtcggatgcg acaggccctt gagcgcctgg
     4141 atgaagcaca cgctggcgac caggtacaac agggtgacga gattgagagt catggcgaag
     4201 cctccggcct gtcggcctgc agtgcgagcg cgctccggac cggcgggcgg gcgctcatgc
     4261 ttgtcgatcc ttgtgcagtt tctcgatcgc cgcggcgagc aggccgacca cggaatccgt
     4321 cacgcccatc gcgccggcca gcaacaggcc ggcgatcagc gccagcggca gccggccgat
     4381 cggctgcggg ccgagctggc gcagcgcgta caggaaaagc gccaccgcgg cgacgagccg
     4441 cacgacgcgc cagaacggcc cgcgccccgg cggcgggcgc tcgtcgccgc ggaggtcggt
     4501 cacgaagcgt ccttggcggg cggacgctcc ttcttccgga acatctccag catccggcgc
     4561 gtgacgagga agccgccgaa cacgttgacc gccgccagcg ccaccgccag cacgcccatc
     4621 gtcttggcga accagccctc ggtcatcgag gccgcgagca tcgcgcccac gatcacgatc
     4681 gccgagatcg cgttggtcac cgccatcagc ggcgtgtgca gcgcgggcgt cacgttccag
     4741 acgacgtgga agccgacgta gatcgccagc acgaagatga tcaggttgac gatggtggga
     4801 gagacgatgt ccatcgggag ctcctcgtgt ggtcaggcgc gccgaacctc gccctcgtgg
     4861 gcgacgaggc aggccgcgac gatgtcgtcc tcgaggtcga gcttgaagcc ggcctccttg
     4921 gtcgtcacca gcttcaggaa gtcgagcacg ttgcgcgcgt agagcgacga cgcgtccgcc
     4981 gcgaccagcg cgggcaggtt ggtctcgccg acgatggtca cgccgtgccg gatgacggtg
     5041 cgcccggcct cggtgagcgg gcagttgccg tcgctgcccg ccgggccgcg gcccgcggcg
     5101 aggtcgacga cgaccgagcc cggcttcatc gcgcgcacca tgtcctcggt cacgagcacc
     5161 ggcgccgcgc ggcccgggat cagggcggtg gtgatcacga cgtccgcctg cgcgacgcgc
     5221 ttggcgacct cgagcttctg gcgatccagc caggactgcg gcatcggccg ggcgtagccg
     5281 ccgacgccct cggccgcctc cttctcctcg gcggtctcgt agggcacgtc gatgaacttc
     5341 gcgccgagcg actcgacctg ctccttcacg ctcgggcgca cgtccgaggc ctcgatgacg
     5401 gcgcccaggc gcttggcggt cgcgatcgcc tgcaggccgg cgacgccgac gcccagcacg
     5461 accacgcgcg ccgccttgat cgtgccggcc gcggtcatca gcatcgggaa caggcgctgg
     5521 tacttgtcgg ccgcgatcat cacggccttg tagccggcga tgttggcctg cgacgagagc
     5581 acgtccatgc tctgcgcgcg cgtcgtgcgc ggggcggctt cgagcgcgaa ggccgtcagg
     5641 cgcgcggcgg ccaggcgctg caggccctcg cggtcgaacg ggttgagcat gccgaccacc
     5701 accgcggccg gcttcatgag gcccagctcg tcggcctgcg gcgcgcgcac cttcagcacg
     5761 agctccgccc cgagcgccgc ggcgcggtcg acgatggacg cgcccgcggc gacgtaggcc
     5821 tcgtcggtgg cgctggcggc caggccggcg cccgactgca cgcgcaccgc gtggcccgcg
     5881 gccgcgagct tcttcgccgt ctcgggggtg acggcgacgc gcgtctcgcc ggccgcggtc
     5941 tccagcggaa ctccgatcaa catgccagtc tcctcgccaa cgacgtcttc cgtggggatg
     6001 cgatgcccgc cgcgcgggca aagtgccgtg cagcttacac aagaacgcgc cggcgtttca
     6061 gtggtgcccg tctactcgcg gcaacagccc gcggcgggcg gcgccgcttt cgctggcggc
     6121 ggcgcggcgc cgacgccaca atcgccgtcc atggcaaaag agcgctggaa gccgagcgtc
     6181 accgtggcgg ccgtcatcga gaaggacggg cgctacctca tgatcgagga gatgacccgc
     6241 gacggcctgc gcctgaacaa ccccgcgggc ggcctcgagg cgggcgagtc gatcctggag
     6301 ggcgcgatcc gcgaggcgct ggaggagacc gcctgccact tcacgcccga ggcgctgctg
     6361 ggcgtctaca tggcgcgcaa tcccgacacc gcgcgcggcc cgggcgtcac ctacgtccgc
     6421 ttcgccttct gcggcagcgc gagcgatccg gtgccggggc gggcgctgga ctcccccatc
     6481 gtgcgcacgc tgtggatgac ggccgacgag atcgtcgccg cgcgcgaccg cctgcgcggc
     6541 ccgctggtgc tgcagtgcat cgaggaccac cgtgccggcc gccgcctgcc gctggacgcg
     6601 atccacaccc atgcgagcgt cgccaacccc gaccccgcgc aggcgacggc cgagccggac
     6661 gacccgcccg cgggcgccgc cgcgccctga ggccgcgccc caagcccttg cggccggccc
     6721 gcgcgccgcg ttttcctaga atgcgggaat gagtgcgatg cgcgtggtgg tcggcctgtc
     6781 cggcggggtg gattcggccg tgacggcgca cctgctcaag cagcaggggc acgaggtcgt
     6841 cggcatcttc atgaagaact gggaggacga cgacgacgac gagtactgct cgtcgcgcca
     6901 ggacttcctc gacgccgcca gcgtcgccga cgtgctgggc atcgagatcg agcacgtcaa
     6961 cttcgccgcc gactacaagg accgcgtctt cgccgagttc ctgcgggagc accgcgccgg
     7021 ccgcacgccc aacccggacg tgctgtgcaa cgcggagatc aagttcaagg ccttcctcga
     7081 ccacgcgatg cgcctgggcg cggacaagat cgccaccggc cactacgcgc gcgtccgcct
     7141 gaacgcggcg accggacggc acgagctgct caagggcctc gacccggcca aggaccagag
     7201 ctacttcctg caccgcctga accagtccca gctcgcgcag acgatgttcc cggtgggcga
     7261 gctgcgcaag accgaggtgc gccgcatcgc cgacgagatc ggcctgccca atgcgaagaa
     7321 gaaggactcg accggcatct gcttcatcgg cgagcgcccg ttccgcgagt tcctgaaccg
     7381 ctacctcgcc cacgacccgg gcccgatcct cgacgaccgc ggccgcaagc tgggcgagca
     7441 cgtcggcctg agcttctaca cgctgggcca gcgccagggc ctgggcatcg gcggggtgaa
     7501 ggacaagcgc ggcgagcgcg gcggcggcga ccacgcgccc tggttcgtgg cgcgcaagga
     7561 gctcgcgacc aacacgctgc gcgtcgtcca ggggcacgac cacccgtggc tgctgtcgcg
     7621 tcgcctcacc gcgaccgacc tcgcctggtg cgccgacctg ccgccggccc ccggcgtgct
     7681 ggccgccaag acccgctacc gacaggccga cgccgcctgc acgctcgagc cgccagccga
     7741 gcccggcagc atcacgctcg acttccccct gccgcagtgg gcggtcactc cggggcagtc
     7801 ggcggtgctg tatgagggcg acgtctgcct cggtggcggc gtcatcgact cggcggtgac
     7861 cgaaggctga cggctgtcac ctgcgcggcg cccgtcggcg tcccttcatg gcatgctccg
     7921 ccaccgctca accgtgccgg aggaggaatc gccatgctcg cccaggtcgc caacgtcgcc
     7981 gtcgcgctca tcgcggcgct gcacgtgtac atcctcgtgc tcgagatgtt cctgtggacg
     8041 acgccccgcg gccggcgcgc gttcgggctg acgccggagt tcgcgcagca gagcaaggcg
     8101 ctcgcggcca accaggggct ctacaacggc ttcctcgcgg ccgggctcgc ctggggcctg
     8161 tggcagggcc cggcgggcgt cgcgacgacg ctgttcttcc tcggctgcgt cctggtggcg
     8221 ggcgtgttcg gcgccgcgac ggccagccgc aagatcctgt tcatccaggc gatcccggcg
     8281 gccatcgcca ttgcactcgt actgctggca ccgtcgcgct gaacgcgctt agtcgaattt
     8341 cgcgcttgct cgtgcgcttc gacttgttgc gcatgtagag gccaattcca taattgcggg
     8401 acgcataacg aaccggcgat ccgtccgccg cgcgtcccat gaacttccag caactgcgct
     8461 ccgtgcgcga agccgtgcgc cgcggcttca acctcaccga ggtcgcccag gcgctgcaca
     8521 cctcgcagcc gggcgtcagc cgccagatcc gcgagctcga ggacgagctg ggcatcgaga
     8581 tcttcgtgcg cgtcggcaag cgcctgaccg gcctgacgca gcccgggcac gccgtgctgc
     8641 cctgggtcga acgcctgctg caggacgccg agagcctgca gcgcgccagc gaggaattca
     8701 cccagggcac caccggcgtc ctgagcatcg ccgcaacgca ctcccaggcc cgctacgcct
     8761 tgccgaccgc ggtgcgcgac ttccgcgcgg cgcatccgaa cgtccagctg cagctgcacc
     8821 agggatcgcc cgagcagatc gcgcagatgc tgctgaccgg caccgtcgac atcggcatcg
     8881 cgtcgcacct gctggcggag cggcccgagc tggtcgccct gccctgctac cgctggacgc
     8941 attcgatcgt cgtgcccgag ggccatccgc tgcaggccga cggcaacgtg accctggaac
     9001 ggctcgcgca gtaccccatc atcacctacg acaccggcct gaccggccgc tggagcatcg
     9061 acgccgcgtt caagcgcgcc ggcctgcggc ccgagatcgt gctgaccgcg atggacgccg
     9121 acgtcatcaa gacctacgtc gacctcgggc tgggcgtggg catcctggca tcgatcgcga
     9181 tcgacgagga cttccccgcc cgcctgcgcg cgatcgatgc gcgcaacctg ttctcgatca
     9241 acaccaccag cgtcgcggtg cgccgcggcg ggctgctgcg cggcttcgcc tacgagttca
     9301 tccagacctt cgcctcgcac ctgagccgcg acgtcgtcga ggcggcgcag cgcgcgccca
     9361 gcgataccga gcaggcgccg ctctgaacgc gggccccgga gccggccccc gcgcccgatt
     9421 cccgggcatt tttgcgacgc tggcggctcg ggacaatccc gaagcgggct caacgcggcc
     9481 gtccgagcgc cgatgatgtc gtcctgggcc gccgaccccg gagaaggccc gccgcgttcc
     9541 caccggtccg ccttcatctc cgtgacgtcc acgcaaccgc ccgccgcccg gcccgacccg
     9601 gggctccgtc ccgaccgggt cgcggacccg gcacgcctgc tcgagcacgc gacctggctg
     9661 ctgtacatcg acggccggcg cgccggcgag ctcggcgtgc aggtgctgga ggcgaccgcc
     9721 cgcgagccga cgcaccgcct gcgcggcgac gcctggtggc acgtcgccta ctcgaagctg
     9781 cgcgcggccg aggtcgaggc cgcgctgcag gccaacgagc aggcgcgcgc ctgctacctc
     9841 gcgcacgacg accagcgcgg cctgctgatg tgcgacgagg tcgacggcct gtacctgcgc
     9901 tcgcgcggcc ggctgcagga ggcgctcgag ctgcacctgc ggatctcggc gcgcaccgat
     9961 gtcgcccgca cgcccgacga cctctacatc acccacaact cgcgcgcgct cacgcgcaag
    10021 cagctgggcc agagcgacgc gatgctgctg gacttctacc aggcgctcgc cgcggcccag
    10081 acctgcgagt cgcccggccc gaacatcaac gcgctcgtca acctcggcgg cagccacggc
    10141 gacctctaca acctgaccga ggcgcagacg ctgtccgagc gggtgctcga cctcgccgag
    10201 gaggccggcg cgtggaacgc gttcgcggtg gcggcgttca acctgatgca ggcctgcgac
    10261 ggcctgggcg cgcgcgagcg ctgcgccgac gtgctcgcgc gcctgcgccg caacgaggtg
    10321 cacatgcccg cgggcatcct gcgccagaac ggcccgtgca tggccatcgc ccacctgtgc
    10381 gccggcgatc tcgagggcgc gcaggcctgg ctggacctcg gcgccaccgc gcccttcgcg
    10441 gacggcgacg gcaagacgga cttcgcgcgc gcccgcgcct gcacgttgat ggcgctgggc
    10501 cgcgacgccg aggcgcgcga ggtcgtcgag gcgcgcatcg cggagtgcgc ccactcgact
    10561 ttccacgatc cgccctacac gcgcatgcgc ctgctgcagg cggccaccga cgcctgcgag
    10621 cgcctgtacg acaccgcggc cgcgctgcgc tacctccacg aggtgcacgc gctgtacgag
    10681 cagctggtcg gccgcagctc gcgcgccggc ttcatcgcgg cgcaggcc
//
LOCUS       DASVLN010000377         8132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_449980, whole genome shotgun sequence.
ACCESSION   DASVLN010000377 DASVLN010000000
VERSION     DASVLN010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449980"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..792
                     /locus_tag="VF453_09215"
     CDS             97..792
                     /locus_tag="VF453_09215"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX7687874.1"
                     /translation="MFRSHCRLRLAIAIACIGLAAGCASPGDDLRQARAAHDIVNRAT
                     VTIATLAEPGARPELRTALHDACAVLIFPHVATVDFIAGGSAGAGVLLVRDAGSQHWV
                     GPAFYAIDQLDVGAQVGLARREVVVVLHRCDGLAALEREGATFRLGTSWGMQAAERGP
                     GVDAAPGVEVFSRTDGVYVGLALRATAVRPDRRLSDAYYDRPLPVAEVLAPGRPCDES
                     SREIRLAVELAAR"
     gene            912..1433
                     /locus_tag="VF453_09220"
     CDS             912..1433
                     /locus_tag="VF453_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEX7687875.1"
                     /translation="MSKVLVVFHSRTGCCRRLAQVLGDTRRWSLGEVDYFEPPGSYWR
                     CALDALTRKEPEIRYQGANPATFDVVVLVSPIWCWGLSPPMRGFVRSMHGKLDRVAVV
                     SCMGGSGAARAVREVEQRIGRRTVARLALTQSQVETGGFDPALQSFADAVEDAASGQG
                     RSIAPAVVAGSPT"
     gene            complement(1481..2179)
                     /locus_tag="VF453_09225"
     CDS             complement(1481..2179)
                     /locus_tag="VF453_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687876.1"
                     /translation="MPTDVIATIRNLRAEAEASLHDVLRHVERARVAGEAADFRGMRT
                     GLFELGGRMSSPRWGIECRLLLPAIAARTPLPRRVARLCADDGSRDLERLAELQEAVT
                     AFELVGEPMHGRLELLALMFTEWHIVRREVFERLVVPLAQSHLTEEDWNLLDAQVGLL
                     LRDDVPVDRPAGGRPRKRAAATGGVASSTVAGAGHDPLLRRRLLAHALRRCEPRAVDR
                     CAEFQREAPALLCA"
     gene            2360..3115
                     /locus_tag="VF453_09230"
     CDS             2360..3115
                     /locus_tag="VF453_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7687877.1"
                     /translation="MSDAASPRSEHAPMRILIVDDHAVVREGLKRMLEASEPSWAIDE
                     AADAFEALDRLRSRTFDVAIVDISMPGMSGLDFLKRVRAPHPGLRVLMLSMYAEEQYA
                     VRAFKSGANGYVTKDSATRELVKAIRKVTAGNVYVSEALAQSLVLGLSGNNGEAPHAQ
                     LSDRELDILRRLAAGDRPTTIANALHISIKTVSTHKARILARLQLPNTAALIRYAMEH
                     GLAPDLASEAGDGPEDDDGATPATGAAGRRASS"
     gene            3160..5670
                     /locus_tag="VF453_09235"
     CDS             3160..5670
                     /locus_tag="VF453_09235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7687878.1"
                     /translation="MSIRARIALLAGVVLLPAALALGWRLADEIQQSRDDALVTVSLV
                     RDDVAERVAWTLDHATKLLAHVAREPEVQAVDLAACRSAIRAVPLLQPSFMRLELRDA
                     AGRLVCATSGEDGLLSFSDGGADPAPVDDPGALEPIVDPATGRLVLPLSQAIVDASGR
                     RRGELRMLLDLRSVSDDLARITGDDAVVSVIDRRMTILMRTRQGADFIGRRTSGWDPT
                     AGRSAGFIEARGSDDVERLYAFSSIPATGWRVFAGMATDRVYAKYEAAVRRTVVLGSV
                     VLLAVVALGWRLVAVIARPVAALQSAVRRVARGKFDSHIEVAGPPELQGVAQDFNRMV
                     DALALSRSRLQALFDTMSEAVIAVDETQVVVTANPAAARLLGVPLPELVGSQLERWIP
                     QRYRGSHREAHDAFIASDSPPRDMGQRPEVSALRADGVEIPVEASISSVRVQGQRFSL
                     AVLRDVGERRKAMAAIAAGKALLAAALANMSDAVLILDAEGRFLATNDAFGTFFRLDE
                     REPPPASIQELASRLEISFEDGRPVPEGRSAGEQAIAGESGSGVLYRLRRRGSDASWV
                     GSFNFAPIRDHDGAITNAVVTARDVTAALAAQRELERSHGALRRLVNALDRSQDDERR
                     RISRELHDDLQQTLAAIGIEASAVLRQMPPAAAALRPGLDRIDSLSRTALRSTRRIIA
                     DLRPQVLEELGLEAALRNMAAVHARHHEVPCEVEIAEDLETEQLPEALANCLYRVAQE
                     ALNNVAKHANAATVRIRLHQTSRDAIVLEVHDDGRGLPPGANAKPRAFGLLGMAERVH
                     ALQGRLRVSPGPDGGTVVEAELPLDPGSEDDEDAAAQA"
     gene            5768..7120
                     /locus_tag="VF453_09240"
     CDS             5768..7120
                     /locus_tag="VF453_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX7687879.1"
                     /translation="MTTIPMHPVDAAWFHMDGPANTAVVTALLTARRRFDPARVKSVL
                     ARRLAPLPAFRRRVADSATWTGAPAWAEDAEVDIDQHVHHRALPAGADGRDLRALVED
                     LASQPLASTLPLWQAHVIDGPGASGALVFRYHHCLGDGAAMMAIARQVLDDDTAAAAA
                     APAPVAVAGAGATSEPLLGIVQAAVGGVGAILRDLVKPGDPPSALKGRFTARQHLAWS
                     RPLPLEPLRTVAHRLGATLNDLAVAAIAGALRGYLVESGQFTDGATMRAMVPVNLRRP
                     GAPAESANAFGLVMLDLPIDRAEPLERLQESIARMSALKRSPEAPAMRELLDLFGRGP
                     KALQQVMQGLLGSKASLVLTNVAGPRQPLHFAGRTIDRLMFWVPHPGEELGLGASVCS
                     YRGRLTFGILADARQVPDPQRIATLVERELDALRRIAAGRRRAATGHPGPAGHRADGR
                     "
     gene            7188..7841
                     /locus_tag="VF453_09245"
     CDS             7188..7841
                     /locus_tag="VF453_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7687880.1"
                     /translation="MKADAELKIDVEAELAWDPSVASPHVTVEVHDGVVTLGGRVASL
                     AEKHAVVEAARRISGVRALAVELAVVLPGHEKRTDVDIAAAARQALDWSTFVPRDQVL
                     AIVEGGRITLHGEVDRQYQRAAAERAVRGLRGVTAVNNLIRLASPLNVPGIEHRIAAA
                     LERQAPAGTPPMLTVRVDGTTVCLHGRVRSWAERDAAAEAAWSAPGVGAVVNDVVVA"
BASE COUNT         1088 a         3001 c         2955 g         1088 t
ORIGIN      
        1 gccggcccgg ggcttgacga tgctcaagcc tgcgggcgcc ctgcggacca agactcgggc
       61 tcccaccgag atccaccacc tgccgaggat cgctccatgt tccgctcgca ttgccggctg
      121 cgcctcgcca tcgccatcgc gtgcatcggt ctcgcggcgg gttgcgcctc gcccggcgac
      181 gatctccggc aagcgcgcgc ggcgcacgac atcgtcaatc gcgccacggt cacgatcgcg
      241 acgctggccg agccgggcgc gcgcccggag ctgcgcaccg ccctgcacga cgcctgcgcc
      301 gtgctgatct tcccgcacgt cgcgacggtc gatttcatcg ccggcggatc ggcaggcgcg
      361 ggcgtgctgc tcgtccgcga tgccggctcg cagcattggg tggggccggc gttctatgcg
      421 atcgaccagc tcgacgtcgg ggcgcaggtc gggctcgcgc gacgcgaagt cgtcgtcgtc
      481 ctgcaccgat gcgacggcct cgcggcgctc gagcgcgagg gcgcgacgtt caggctcggc
      541 acctcctggg gcatgcaggc ggcggaacgc ggtcccggcg tggacgccgc gccgggcgtc
      601 gaggtgttct cgcggacgga cggggtctac gtcggcctcg ccctgcgagc gacggccgtc
      661 cgtccggacc ggcggctgtc cgacgcctac tacgaccgcc cgctgccggt cgccgaggtg
      721 ctcgcgcccg ggcggccctg cgacgagtcg tcccgcgaga tccgcctggc cgtcgaactc
      781 gcggcccgat gagcgtcgtc gcccgctccc gggcgtgacg aggcggccga tccgcgtccg
      841 gattgcgccg gcgcaacccg cgccgcgcgg ggctgcgaca gattgacggc agcctgtcgc
      901 aggagcccgc catgtccaag gtgctggtcg tcttccactc ccgtaccggt tgctgccgcc
      961 gcctggcgca ggtcctgggc gatacgcgtc gctggtcgct cggcgaggtc gattacttcg
     1021 agccgcccgg cagctactgg cgatgcgccc tggacgcctt gacgcgcaag gaaccggaga
     1081 tccgctacca gggcgcgaac ccggcgacct tcgacgtcgt ggtgctcgtc tcgccgatct
     1141 ggtgctgggg gctgtcgccg cccatgcggg gcttcgtgcg ctcgatgcat ggcaagctcg
     1201 accgcgtcgc cgtggtctcg tgcatgggag gaagcggcgc ggcacgcgcg gtgcgcgagg
     1261 tcgaacagcg catcggccgc cgcacggtcg ccaggctggc attgacgcag tcgcaggtcg
     1321 agaccggtgg attcgaccct gcgctgcagt ccttcgcgga cgccgtggag gacgccgcat
     1381 cgggccaggg gcgcagcatc gcgccggcgg tcgtcgccgg gtcgccgacc tgacggccgg
     1441 cggcgagcgc agcggtcgag cctgagtcgc gacggcaacg ctaggcgcag agcagcgcgg
     1501 gagcctcgcg ctggaactcc gcgcaccggt cgaccgcccg gggctcgcaa cgccgcagcg
     1561 cgtgggccag caatcggcga cgcagcagcg gatcgtgccc cgcgccggcg acggtggagg
     1621 acgcgacgcc gcccgttgcg gcggcgcgct tgcgcggccg gccgccggcg ggccggtcca
     1681 ccggcacgtc gtcccgcagc agcaggccga cctgggcgtc gaggaggttc cagtcctcct
     1741 cggtcaagtg cgactgcgcc aggggcacga cgaggcgctc gaacacctcg cgccgcacga
     1801 tgtgccactc ggtgaacatc agcgcgagca gctcgagacg cccgtgcatg ggctcgccga
     1861 ccagctcgaa ggcggtcacc gcctcctgca gctccgccag gcgctcgagg tcgcgcgaac
     1921 cgtcgtccgc gcacaggcgc gccacgcggc ggggcagcgg cgtccgcgcc gcgatcgcgg
     1981 gaagcaggag ccggcactcg attccccagc ggggggagga catccgcccc ccaagttcga
     2041 acaaccccgt ccgcatgccc cggaagtcgg ccgcctcgcc ggccaccctc gccctctcga
     2101 cgtgtcgcag gacgtcgtgg agcgaggctt cggcctcggc gcgcaggttg cggatggtgg
     2161 cgatcacgtc ggtcggcatc ggcggctccc cttgtacgag agcacagctt cgggccgcgg
     2221 acgccgatcc gattgcttgc gctcaatcgt cgcgcttgcg cccgatcaag cgcgcgctag
     2281 gatctcgacc tcacactcgg ccgcgatcga tctccccagc ggctcgccga cgcgcgggca
     2341 aagaggatcc acgacgccca tgtccgacgc cgccagcccg cgttccgagc acgccccgat
     2401 gcgcatcctc atcgtcgatg accacgcggt cgtgcgcgag ggcctgaagc gcatgctcga
     2461 agccagcgag ccctcgtggg cgatcgacga ggccgccgac gcgttcgagg cgctcgatcg
     2521 cctgcgcagt cgcaccttcg acgtcgcgat cgtcgacatc tcgatgcccg gcatgagcgg
     2581 actcgacttc ctgaagcggg tccgggcgcc ccatcccggg ctgcgcgtgc tcatgctcag
     2641 catgtatgcg gaggagcagt acgccgtgcg cgccttcaag agcggcgcca acggctacgt
     2701 gacgaaggac tccgcgacgc gcgagctggt caaggcgatc cgcaaggtca ccgcggggaa
     2761 cgtgtacgtc agcgaagcgc tcgcgcagag cctggtgctg ggcctgtccg gcaacaacgg
     2821 cgaggcgccg cacgcccagt tgtccgaccg cgagctggac atcctgcgac ggctggcggc
     2881 gggcgatcga cctacgacga tcgccaacgc cctgcacatc tcgatcaaga cggtcagcac
     2941 gcacaaggcg cgcatcctcg cgcgcctgca gttgcccaac acggcggcgc tgatccgcta
     3001 cgccatggag cacggactgg cgccggacct ggcctcggag gccggcgacg gaccggaaga
     3061 cgacgacggc gcgacgccgg caacgggcgc ggccggtcga cgggcatcgt cgtgacggcc
     3121 tggccggacg tcccgagccc cgagtcctcc gcgcccggga tgtcgatccg cgcccggatc
     3181 gcgctgctcg ccggggtcgt gctgctgccc gcggccctgg cgctgggctg gcggctggcc
     3241 gacgagatcc agcaatcccg cgacgacgcc ctggtgaccg tctcgctcgt gcgcgacgac
     3301 gtggccgaac gggtggcctg gacgctggat cacgcgacga agttgctggc gcacgtcgcc
     3361 cgcgagccgg aagtccaggc cgtcgacctc gcggcctgcc gctccgccat ccgcgcggtt
     3421 cccttgctgc agccctcgtt catgcgactg gagttgcgcg acgccgcggg ccggctcgtg
     3481 tgcgcgacga gcggcgagga cggcctcctc agcttctcgg acggcggcgc cgacccggcg
     3541 cccgtggacg acccgggcgc gctggagccg atcgtcgatc cggccacggg gcgcctcgtc
     3601 ctgccgctct cgcaagccat cgtggacgcc tccggccggc gacgaggcga gctgcgcatg
     3661 ctgctcgacc tgcgcagcgt gagcgacgat ctcgcccgca tcacgggcga cgatgccgtg
     3721 gtcagcgtca tcgaccgccg catgacgatc ctgatgcgca cgcggcaggg cgcggacttc
     3781 atcgggcgtc gaacctcggg ctgggatccc accgccggac gctcggccgg cttcatcgag
     3841 gcgcgcggca gcgacgacgt cgagcgcctg tatgcgttct cgtcgatccc ggcgaccggc
     3901 tggcgcgtgt tcgccggcat ggccaccgac cgggtctatg ccaagtacga ggccgcggtg
     3961 cggcgcacgg tcgtgctggg cagcgtcgtc ctgctggcgg tcgtcgcgct cggctggcgc
     4021 ctggtggcgg tcatcgcccg tccggtcgcc gcgctgcagt cggcggtgcg ccgggtcgcc
     4081 cgcggcaagt tcgacagcca tatcgaggtc gccggcccgc ccgagctcca gggcgtggcg
     4141 caggacttca atcgcatggt cgacgccctc gcgctgagcc gatcgcgcct gcaggcgctc
     4201 ttcgacacga tgtccgaggc ggtcatcgcg gtcgacgaga cgcaggtggt cgtgacggcc
     4261 aatcccgcgg ccgcccggct gctgggcgtt ccgctgccgg aactggtggg ctcgcagctg
     4321 gaacgctgga ttccgcagcg ataccgcggc tcgcaccgcg aggcccacga tgccttcatt
     4381 gcaagcgact ccccgccgcg cgacatggga cagcgcccgg aggtcagcgc cctgcgcgcc
     4441 gacggcgtcg agattcccgt cgaagcctcg atctcgtccg tgcgcgtgca gggccaacgc
     4501 ttctcgctgg cggtcctgcg cgacgtcggc gagcgacgca aggcgatggc cgcgatcgcg
     4561 gccggcaagg ccctgctcgc ggccgcgctc gccaacatga gcgacgccgt cctgatcctc
     4621 gacgccgagg gccgcttcct cgcgaccaac gacgccttcg gcacgttctt ccgcctcgac
     4681 gagcgcgaac ccccgcccgc gagcatccag gaactggcct cgcggctgga gatctccttc
     4741 gaggacggcc ggccggtgcc cgagggacga agcgcgggcg agcaggcgat cgccggggaa
     4801 tcgggctccg gcgtgctgta tcgcctgcgc cggcgcggga gcgacgcgag ctgggtcggc
     4861 agcttcaact tcgcgccgat ccgcgaccac gacggcgcca tcaccaacgc ggtcgtcacc
     4921 gcgcgcgacg tgacggcggc gctggccgcg caacgcgagc tcgaacgatc gcatggcgcg
     4981 ctgcggcgcc tggtgaatgc gctcgaccgc tcgcaggacg acgagcggcg ccggatctcg
     5041 cgcgagcttc atgacgacct gcagcagacg ctggcggcga tcggcatcga ggcttccgcg
     5101 gtcctgcggc agatgccccc ggcggccgcg gcgctgcgac cgggactcga tcgcatcgac
     5161 agcctctcgc gcaccgcgct gcgctcgacg cgccgcatca tcgccgacct gcgtccccag
     5221 gtgctcgagg agctcggcct ggaagcggcg ctgcgcaaca tggccgcggt ccatgccagg
     5281 caccacgagg tgccgtgcga ggtcgagatc gcggaggatc tcgagaccga gcagctcccc
     5341 gaggccctgg cgaactgcct ctaccgcgtc gcccaggaag ccttgaacaa cgtcgccaag
     5401 catgcgaacg ccgccaccgt ccgcatccgg ctgcaccaga cgtcgcgcga cgccatcgtg
     5461 ctggaagtcc acgacgacgg gcgcggcctg ccgcccggcg ccaacgcgaa gccgcgcgcc
     5521 ttcggcctgc tgggcatggc cgagcgcgtg catgcgctgc aggggcggct gcgggtgtcg
     5581 cccgggcccg acggcggcac ggtggtcgag gcggagctgc cgctcgatcc ggggtccgaa
     5641 gacgacgagg atgccgcggc gcaggcgtga ggcggcggcg cgtcccattg cagggccgcg
     5701 tcgccgtgcc ggccgcttgc gcccgcgcaa gcggagcgcc ggccgccgcg gctatcgtgg
     5761 gttcgtcatg acgacgatcc cgatgcatcc cgtcgacgcc gcctggttcc acatggacgg
     5821 gccggcgaac accgcggtcg tgaccgcgct gctcacggcc cggcggcgct tcgatccggc
     5881 gcgcgtgaag tcggtgctgg cgcgccggct cgcgccgctg ccggcgttca ggcgtcgcgt
     5941 cgcggactcc gccacctgga cgggcgcgcc ggcctgggcc gaggacgccg aggtcgacat
     6001 cgaccagcac gtccaccatc gcgccctgcc cgcgggagcc gatgggcggg acctgcgcgc
     6061 cctggtcgag gatctcgcca gccagccgct cgcctcgacg ctgccgctgt ggcaggccca
     6121 cgtgatcgac ggcccgggcg cgagcggcgc gctcgtgttc cgatatcacc actgcctggg
     6181 cgacggcgcc gccatgatgg ccatcgcgcg gcaggtcctg gacgacgaca ccgcagccgc
     6241 cgcggcggcg ccggcgcccg tggcggtcgc gggcgcgggc gcgacgtccg agcccctcct
     6301 gggcatcgtg caggcggccg tcgggggcgt cggcgcgatc ctgcgcgatc tcgtgaagcc
     6361 cggcgatccg ccttcggccc tcaagggacg gttcacggcg cgccagcacc tggcgtggtc
     6421 ccgtccgctg ccgctggagc cgctcaggac cgtcgcccat cggctcggcg cgacgctcaa
     6481 cgacctcgcc gtcgcggcga tcgccggcgc cctgcgcggc tacctggtgg agagcgggca
     6541 gttcacggac ggcgcgacga tgcgcgcgat ggtgccggtc aacctccggc ggcccggcgc
     6601 gccggccgaa tcggccaacg ccttcggcct cgtgatgctg gacctgccga tcgaccgcgc
     6661 ggagcctctg gagcggctgc aggagtcgat cgcacggatg tcggcgctga agcggtcgcc
     6721 cgaggcgccc gcgatgcgcg agctgctcga tctcttcggc cgcgggccca aggcgctgca
     6781 gcaggtgatg cagggactgc tcggctccaa ggccagcctc gtcctcacga acgtcgccgg
     6841 cccgcggcag cccctgcact tcgcaggccg cacgatcgac cgcctgatgt tctgggtgcc
     6901 gcatcccggc gaggaactgg gactgggcgc gagcgtctgc agctaccgcg gacggctgac
     6961 cttcggcatc ctcgccgacg cccggcaggt gcccgacccc cagcggatcg ccaccctggt
     7021 cgagcgcgag cttgacgcgc tgcgacggat cgcggccggc cggcgccgcg cggcgaccgg
     7081 gcacccgggc ccggccgggc atcgcgccga cgggcgttga cggcgcgcaa tcgccgcggc
     7141 ggtcgccgtt cctatcgtgg acgggaacga ccactcgggg gtgcaccatg aaggccgatg
     7201 ctgagctgaa gatcgacgtc gaggcggaac tggcctggga tccgtcggtc gcctcgccgc
     7261 acgtcacggt cgaggtgcac gacggcgtcg tcacgctggg cggccgcgtc gccagcctgg
     7321 ccgagaagca cgccgtcgtg gaagcggccc ggcggatcag cggcgtgcgc gcgctggccg
     7381 tcgagctcgc cgtggtgctg ccaggccacg agaagcgcac cgacgtcgac atcgcggccg
     7441 ccgccaggca ggcgctcgac tggagcacct tcgtgccgcg cgaccaggtg ctggcgatcg
     7501 tggaaggcgg ccgcatcacg ctccacggcg aggtcgaccg gcagtaccag cgcgccgccg
     7561 cggaacgcgc ggtgcgcggg ttgcggggcg tgacggccgt caacaacctc atccgcctgg
     7621 cgtcgcccct caacgtgccg ggcatcgagc atcgcatcgc ggccgccctg gaacgccagg
     7681 cccccgccgg cacgccaccg atgctgaccg tgcgcgtcga cggcacgacc gtgtgcctgc
     7741 acgggcgcgt ccggtcctgg gccgagcgcg acgccgcggc cgaggcggcc tggtcggcac
     7801 cgggcgtagg cgccgtcgtc aacgacgtcg tcgtcgccta gtgtcgtgat cgagaagttc
     7861 gttgaacagg tctcgagcgc agaggcggcg tcgatgaatt ctttcgcaat cgggcgcaga
     7921 ctaggcgccg cgcgcagcga ggctcaatgc ctcgcgagca cggcaacgac ggctgcgatc
     7981 gatttcggaa gaattcttca acgaacttct cgatcacgac actagagggg tgccgcgtgg
     8041 ccggattcga ctgggcgtgc tggtggacgc tcgtgctgga cgccgccgcc gcgccggcgc
     8101 gcgatccggc ggcggcgagg ctcgccaccc ac
//
LOCUS       DASVLN010000378         4915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_181142, whole genome shotgun sequence.
ACCESSION   DASVLN010000378 DASVLN010000000
VERSION     DASVLN010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181142"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VF453_09250"
     CDS             complement(<1..1374)
                     /locus_tag="VF453_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEX7687881.1"
                     /translation="MSFRATVGHQVFGFADLRELMAKASPARSGDRLAGIAAESARER
                     VAAQAALADVPLARFLSDAVVPYESDEVTRLIVDSQDASAFAPVRHLTVGAFRDWLLS
                     DVATTAALAALAPGLTPEMVAAASKLMRNQDLILVASRCRVVTRFRNTLGLPGSLAVR
                     LQPNHPTDDPAGILASIVDGLMYGAGDAVIGINPAADSVAQLVRLVHLLDELVAGQAI
                     PTQSCVLTHVTNMLRAIELGAPVDLVFQSIAGTEAANRGFGVSLALLDEAHAAALALA
                     RGTTGTDVMYFETGQGSALSAGAHHGVDQQTLEARAYAVARRYRPLLVNTVVGFIGPE
                     YLYDGKQIVRAGLEDHFCGKLLGLPMGCDVCYTNHAEADQDDMDTLLVLLATAGLNFM
                     MGVPGADDVMLNYQSTSFHDALVVRRLLGLRRAPEFEAWLHARGITDERGQLRPVAGL
                     PAFASSVG"
     gene            complement(1502..1885)
                     /locus_tag="VF453_09255"
     CDS             complement(1502..1885)
                     /locus_tag="VF453_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687882.1"
                     /translation="MAAGVVALAVVTIAQAADGIDKKRREPVDVPPLVAGGLKYEDPR
                     LGSPFGYAQDGGVVVARLADSGELVWTRLVYRVAHDPGIEGDKQDVFIKGLTLTEDGK
                     RLVVVNERGQRFDMNLDGSGLRAVP"
     gene            complement(1909..2250)
                     /locus_tag="VF453_09260"
     CDS             complement(1909..2250)
                     /locus_tag="VF453_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687883.1"
                     /translation="MAADKKRREPADVQPVEHDGLRYEAPRHGTPFGYHQDGGFITAR
                     DAATGALVWSQRVYVVDYGDDIESDKQDVFVKAVTLSGDARALLIVDERGERYRLGLV
                     DRSVERLERAE"
     gene            2732..3601
                     /locus_tag="VF453_09265"
     CDS             2732..3601
                     /locus_tag="VF453_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687884.1"
                     /translation="MLTVFSSAATAVALLGCLAWMLRTWLSQRLKSGIQHEYDARLAS
                     LNSALKEQTDAASSKLNSSLKEQTDAAASRLKAAFDRESDKLQLVSQSLGEAQKSAIS
                     KRLLAIEKTWLAALQISDAVPPAVSTLDVLTETEIVEAHNYPNYLMQLNALDAVKVSR
                     PIMEFTRNLAEQRPYIGEYLWALYGVYQAVALRIIYLVDASRGDPKRRLWSQDKVIRQ
                     NIESALGKSELDAFDAQLGGKYLWIQDRFSRAILKATEKIIAGSEFSEAALRQAEIME
                     ERIRQSSLEKQKN"
     gene            3945..4514
                     /locus_tag="VF453_09270"
     CDS             3945..4514
                     /locus_tag="VF453_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687885.1"
                     /translation="MSAIDDLRNALISHPAIKAVKDCPQVSVAVGQAMYGKIQSDGKG
                     EVVKADASDGALARLLGFGGAPEDTTLWHFCTGPAVHHFVVIPAQNDERQWRYLVLMA
                     YEKTDGPGGNPGYSVKQYVERTCNLSAGYKDSWTSSDLVVMLRALFSDRTTWGRYFLN
                     GKDTEVKEITCYKYPGISVKKALQNLAKG"
BASE COUNT          883 a         1626 c         1610 g          796 t
ORIGIN      
        1 gccgacgctg gacgcgaagg ccggcagccc ggccacgggc cgcagctggc cgcgctcgtc
       61 ggtgatgccg cgcgcgtgca gccaggcctc gaactcgggc gcgcgccgca ggcccagcag
      121 gcggcgcacg acgagggcgt cgtggaacga ggtgctctgg tagttgagca tcacgtcgtc
      181 cgcgcccggc acgcccatca tgaagttcag gcccgcggtc gcgagcagca ccagcagcgt
      241 gtccatgtcg tcctggtcgg cctcggcgtg gttcgtgtag cagacgtcgc agcccatcgg
      301 caggcccagc agcttgccgc agaagtgatc ctcgaggccc gcgcgcacga tctgcttgcc
      361 gtcgtagagg tactccggac cgatgaagcc gaccaccgtg ttgaccagca gcggccggta
      421 gcgccgcgcg accgcgtagg cgcgtgcctc gagcgtctgc tggtcgacgc cgtggtgcgc
      481 gccggccgac agcgcgctgc cctggccggt ctcgaagtac atgacgtcgg tgcccgtcgt
      541 gccgcgcgcg agcgcgagcg ccgcggcatg cgcctcgtcc agcagcgcga gcgacacgcc
      601 gaagccgcgg ttggccgcct ccgtgccggc gatggactgg aagacgagat ccaccggcgc
      661 gcccagctcg atcgcgcgca gcatgttcgt gacatgggtg agcacgcacg actgcgtcgg
      721 gatcgcctgc cccgcgacca gctcgtcgag caggtgcacg agccgcacca gctgcgcgac
      781 gctgtcggcc gccgggttga tgccgatgac cgcgtcgccg gcgccgtaca tcaggccgtc
      841 gacgatgctc gcgaggatgc cggcggggtc gtcggtcgga tggttgggct gcaggcgcac
      901 ggcgagcgag ccgggcaggc cgagcgtgtt gcgaaagcgc gtgacgacgc ggcagcggct
      961 ggcgacgagg atcaggtcct ggttgcgcat cagcttgctc gcggccgcga ccatctccgg
     1021 cgtcaggccg ggcgcgagcg cggcgagcgc ggccgtcgtc gccacgtccg acagcaacca
     1081 gtcgcggaac gcgccgaccg tgaggtggcg caccggcgcg aaggccgagg cgtcttgcga
     1141 atcgacgatc aggcgcgtga cttcgtcgga ctcgtagggc acgaccgcgt ccgagaggaa
     1201 gcgcgcgagc ggcacgtccg ccagcgcggc ctgcgcggcg acgcgctcgc gcgccgactc
     1261 ggccgcgatg cccgcgagcc ggtcgccgga gcgcgcgggg ctcgccttgg ccatcagctc
     1321 gcgcaggtcc gcgaagccga agacctggtg accgacggtg gcgcggaagc tcatcgcgcg
     1381 acgataccgt caagggcgct gtcgcgacaa gggcgacgcg atcgcgccgg ccgcgaatgc
     1441 aggggcttcc catagcggcg ccgccaatct tcctcagcgg gtgctgctgc gttgaagcca
     1501 gtcagggcac ggcgcgcaag ccactgccgt ccaggttcat gtcgaaccgc tggccgcgct
     1561 cattgacgac gacgaggcgc ttgccgtcct cggtcaacgt caagcccttg atgaagacgt
     1621 cctgcttgtc tccctcgata cccggatcat gtgcaaccct gtagacgagg cgcgtccaca
     1681 ccagctcacc cgaatcggca aggcgcgcca caacgacgcc gccgtcctgc gcgtagccga
     1741 acggcgagcc caggcgagga tcctcgtact tcaggccgcc ggcaaccagg ggcggcacgt
     1801 cgacaggttc ccggcgcttc ttgtcgatgc cgtcggcggc ctgcgcgatg gtgaccaccg
     1861 ccagcgccac gacgcccgcg gccatgcgag agatcgagga acgcgccgtc attcggcgcg
     1921 ctcgaggcgc tcgaccgagc ggtcgaccag ccccaaccga tagcgctcgc cgcgctcgtc
     1981 gacgatcagc aacgcacgag cgtcgccgga tagcgtcacg gccttgacga agacatcctg
     2041 cttgtcgctc tcgatgtcgt cgccgtagtc cacgacatag accctttgcg accatacgag
     2101 tgcccccgtt gccgcatcac gcgccgtgat gaagccgccg tcctggtgat atccgaatgg
     2161 cgttccatgc cgcggagcct catagcgcag gccgtcgtgc tccacgggtt gcacatccgc
     2221 cggctcgcgc cgcttcttgt ctgctgccat gtggagttcc agcgtatcag ggattttgaa
     2281 tcgatcggat gtaggccgca cgcagagcca gaggctcctt gagcaccttc actgcggcga
     2341 cctccgctgg cgtcccccgc tcgaggcaac gatcggtcga ccgcccgcct cgtgccgccc
     2401 gcgcaccgcg aagacgttct tgccgaggcc cacgcgaccg atcagaatcc ggggcgactt
     2461 ccgactcccc gcaatcgggg tcaagtctcg cctgacaaat cacgagcgac gggactgccg
     2521 gcgtcttgag cggcgatgcg tcaactgacc ggcggatcgc ttgattgcct ggcgagactt
     2581 gaccccaatg tcgaaggtag tgaagggcgt cgaagacgcg tttcgagccg aaccttagtt
     2641 tgggaatact ggggaaattg gtgttgcgcc tcacactcgt ggcgtccagg ttcgagagga
     2701 tggtctgaga atgatggatt tcccaagctt catgctcacg gtgttcagct cagcggctac
     2761 ggcggtcgcg ctgctcggtt gtctcgcttg gatgttgcgg acgtggctat cgcagaggct
     2821 caagtcaggc atccagcacg aatatgacgc tcgattggct tcactgaact ccgcgctcaa
     2881 agaacagacc gacgcggcat cctccaaatt gaactcttcg ctgaaggagc aaactgatgc
     2941 tgcggcgtcc agactaaaag ctgcattcga ccgcgagagc gacaagcttc aactggtttc
     3001 gcaatctctg ggcgaggccc agaaatccgc catttcgaag cgactattgg ccattgagaa
     3061 aacctggctg gcggcgcttc aaatctccga cgcggttcct ccagcagtgt ccacactaga
     3121 tgtactcact gagacagaga tcgtcgaggc tcacaactac ccaaactacc taatgcagtt
     3181 aaatgcattg gatgccgtga aggtaagtcg ccctatcatg gagttcacgc gcaatcttgc
     3241 tgagcaacgt ccctacatcg gagagtacct gtgggcactc tatggcgtct accaggctgt
     3301 tgcccttcgc atcatctatc ttgttgacgc gagcaggggg gacccaaaga ggcgtctctg
     3361 gagtcaagac aaggtgatcc gccaaaacat tgagtcggcg cttggcaagt ccgaacttga
     3421 tgcctttgat gctcaactcg gcgggaagta cctatggatt caggacagat tttcgagagc
     3481 gatcttgaag gcgacggaga agattatcgc gggcagtgag ttcagtgagg ccgcattgag
     3541 acaagcggaa atcatggagg agcggattcg ccaaagttct ctcgagaagc agaaaaattg
     3601 atctagtcca ccgctggata gatgtcgcct ctgccgcaga agcggcgggt cggagtagcc
     3661 gctgtcgaac gtcggctttc aggctgtcag ttctccgctg cagtgagctg ccctggatcg
     3721 aaagccccaa ttcgcacccg cgagccgcgg ccctactgcc caccacccgc cgaacgccgc
     3781 gtcgtcggca acgcagaagt ccagacctga cgcccggaca cgcgccgtcc tcgatcggaa
     3841 ctaacatccg cgacgccttt cccggtcagg ccgatccacg gccgtcagga tcggcggcgg
     3901 tgcggcacgg cgtgccgatc attttttcaa gggagaactt caacatgagc gcgatcgacg
     3961 acctcaggaa cgccctgatt tctcatccgg ccatcaaggc cgtcaaggac tgcccgcagg
     4021 tgtccgtcgc ggtcggccag gccatgtacg ggaagatcca gagcgacggc aagggcgagg
     4081 tcgtcaaggc ggatgcgagc gatggcgcgc tggcgcgtct gctcggcttc ggcggcgcgc
     4141 ccgaagacac caccctgtgg cacttctgca ccggccccgc cgtgcatcat ttcgtggtga
     4201 tcccggcgca gaacgatgag cggcaatggc gctacctggt gctcatggcg tacgagaaga
     4261 ccgacgggcc cggggggaat ccgggctatt ccgtcaagca atacgtcgaa cggacctgca
     4321 acttgagcgc gggctacaag gactcgtgga cgtcgagcga cctggtcgtc atgctgcggg
     4381 cccttttcag cgaccggacg acctggggac ggtatttcct gaacggcaag gataccgagg
     4441 tgaaggagat cacctgctac aagtaccccg gcatctcggt caagaaggca ctgcagaacc
     4501 tcgcgaaggg ttgaggcgga gtcctccgcc gccggaggca gccggcacgc ccggcgaatg
     4561 cgtccctggg aaccgtcacc ggctcccgcg gacccgccgt cccgtccctg ggaggcattc
     4621 atgggccctg gggccaggat gatgcctcgg aaggactcga gttcctggct tcgagacgag
     4681 ccgccggttc cgagagaccc atttccgcac caaattggcg catgcagggg tcttcgcgct
     4741 ggaacggccg ggccccggca cccacggacg ggtacttccg tggacgaacc gctcccggag
     4801 acccatagcc ggttcccgga gacccatcgg cagtccccgg ggacccatcg ccggctcccc
     4861 gagacccatc gccggctccc cggcagcccc ggccgcctcg cgcggccccg cgggc
//
LOCUS       DASVLN010000379         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_73123, whole genome shotgun sequence.
ACCESSION   DASVLN010000379 DASVLN010000000
VERSION     DASVLN010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73123"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..467
                     /locus_tag="VF453_09275"
     CDS             99..467
                     /locus_tag="VF453_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7687886.1"
                     /translation="MFDHVVFGVRDYAASKAFYLQALAPLGLEVVAEGPLGLEMSADG
                     KTSLCIFRTDEKPAHLHLAFRAADRAQVDAFHRAALAAGGTDNGAPGLRPRYHANYYA
                     AFVIDPDGHNVELVCHEPAA"
     gene            577..897
                     /gene="sugE"
                     /locus_tag="VF453_09280"
     CDS             577..897
                     /gene="sugE"
                     /locus_tag="VF453_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEX7687887.1"
                     /translation="MAWLLLVVAGLLETGWAIGLKYAEGFTRPVVSALTLAAMVLSVV
                     LLGIAMKSLPVGTSYAVWVGIGAVGTAILGMVLFAEPASAGRLASLALIVAGIVGLKL
                     TNAG"
     gene            complement(898..1671)
                     /gene="xth"
                     /locus_tag="VF453_09285"
     CDS             complement(898..1671)
                     /gene="xth"
                     /locus_tag="VF453_09285"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7687888.1"
                     /translation="MRIATFNVNGVKGRLPRLLEWLDETTPDVACLQEIKTGDATFPH
                     EPIEAAGYRALWHGQPRHHGVAVLSRADAPREVRRGLPGDDADGQARYLEVDVGPLRV
                     ASVYLPNGNPVPSDNFDYKLRWFERFIAHAASLVDGKRDVVLCGDFNVVPTNEDIYNA
                     GSWRFDAVLQPETRAAWQRLLAQGWVDSARRLHPAGRRELYTFWVDESAFRRHAGFRM
                     DFVLLSPGLRARLAACGVDADQRERDKPSDHAPMWVELR"
     gene            complement(1694..2125)
                     /locus_tag="VF453_09290"
     CDS             complement(1694..2125)
                     /locus_tag="VF453_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7687889.1"
                     /translation="MLNKSVLLVEDDALDAEMTKSAVESVGDCTVTVVVNGQAALARL
                     RETDYEREGAAPAPSLILLDLVLPLVDGLEVLREVRRHAQSRRIPIVVFTSSQREAEV
                     YLSYQLGANAFVIKPLDGSQFRRVVEHTVRFWLHSNVLPPH"
     gene            complement(2180..>3795)
                     /locus_tag="VF453_09295"
     CDS             complement(2180..>3795)
                     /locus_tag="VF453_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7687890.1"
                     /translation="RDTARRVAAGGSAHAAAPDVRRTPNLSQEKRLAAQRIAQALRER
                     IATLADLGLGYLSMERATPTLSPGELQRLRLATQIHSNLFGVVYVLDEPTAGLHPADS
                     EALLRALDRLKAAGNSLFVVEHDLDTIRRADWIVDVGPGAGTQGGEVLYSGPPAGLRG
                     VAASRTAQYLFEPPDARTRPPRAPKAWLELRGVTRNNLRALDVRFPLGVFTAVTGVSG
                     SGKSSLVSQALVELVAAHLGREPEDADDEPEAGAPEVVVPTGGRLHAGAHHVRRLVRV
                     DQKPIGRTPRSNLATYTGLFDAVRKLFAATPAARKRRYDAGRFSFNVAKGRCATCEGE
                     GFVGVELLFMPSVYAPCPACHGARYDDETLQVRWNGRNIADVLALTVDEAVDVFDGEP
                     AIRRPLALLRDIGLGYLRLGQPATELSGGEAQRIKLATELQRPQRGDTLYVLDEPSTG
                     LHPADVDRMMAQLQGLVDAGNTVVVVEHEMRLVAAADWIVDMGPGAGEAGGRVVACGT
                     PAEVARDGAGPTGRYLRAALSAPARSDAV"
BASE COUNT          508 a         1365 c         1413 g          509 t
ORIGIN      
        1 cggccgcgcg ccgcgccgac ccgatcccgc ggcgatctaa actcgcgagc gtcacgccgc
       61 ggccccgcgc cggcccggcg ccctctgcag gagagcccat gttcgaccac gtcgtctttg
      121 gagtccgcga ctacgccgcc agcaaggcgt tctacctcca ggccctcgcg ccgctgggcc
      181 tcgaggtcgt cgcggagggg ccgctcggcc tcgagatgag cgccgacggc aagacctcgc
      241 tgtgcatctt ccgcaccgac gagaagccgg cgcacctgca cctggcgttc cgggccgctg
      301 atcgcgcgca ggtcgacgcc ttccaccgcg ccgcgctggc ggcgggcggc acggacaacg
      361 gcgcgcccgg cctgcgtccg cgctaccacg cgaactacta cgccgcgttc gtgatcgatc
      421 ccgacgggca caacgtcgag ctcgtctgcc acgagcccgc ggcctgagcg agggccctgg
      481 cgaagtcggc ccggtcgcac gcgggctctc gcggccttgc gccgcggcga acgccagcgc
      541 gacaatcgcc gcttcatcga attgaggaag aagcgcatgg cgtggttgct gctggtcgtc
      601 gccggcctgc tcgagacggg ctgggccatc gggctgaaat acgccgaggg gttcacgcgc
      661 ccggtggtca gcgcgctgac gctggcggcg atggtgctca gcgtcgtgct gctgggcatc
      721 gcgatgaagt cgctgccggt gggcacgtcc tacgccgtgt gggtcggcat cggcgccgtg
      781 ggcaccgcga tcctcggcat ggtgctattc gccgagcccg cctccgcggg gcgcctcgcg
      841 agcctcgcgc tgatcgtcgc gggaatcgtg ggcctcaagc tgacgaacgc cggctgatca
      901 gcgcagctcg acccacatcg gcgcgtggtc gctgggcttg tcgcgttcgc gctggtcggc
      961 gtccacgccg caggcggcca ggcgggcgcg caggccgggg ctgagcagca cgaagtccat
     1021 gcggaagccg gcatggcgcc ggaacgcgga ctcgtcgacc cagaaggtgt agagctcgcg
     1081 ccgcccggcc gggtgcagac ggcgcgcgct gtcgacccaa ccctgcgcca ggaggcgctg
     1141 ccaggccgcg cgcgtctcgg gctgcagcac cgcgtcgaag cgccacgagc cggcgttgta
     1201 gatgtcctcg ttggtcggga cgacgttgaa gtcgccgcac agcacgacgt cgcgcttgcc
     1261 gtcgacgagc gacgccgcgt gggcgatgaa gcgttcgaac cagcgcagct tgtagtcgaa
     1321 gttgtcgctc ggcaccggat tgccgttggg caggtagacc gacgcgaccc gcagcgggcc
     1381 gacgtcgacc tcgaggtagc gcgcctggcc gtcggcgtcg tcgccgggca gcccgcgccg
     1441 cacctcgcgc ggcgcgtccg cgcgcgagag caccgccacg ccgtgatgcc gcggctgtcc
     1501 gtgccacagc gcgcggtagc cggcggcctc gatcggctcg tgcgggaagg tcgcgtcgcc
     1561 ggtcttgatc tcctgcaggc aggcgacgtc gggcgtcgtc tcgtccagcc attcgagcag
     1621 ccgcggcagg cgccccttga cgccgttgac gttgaacgtg gcgatcctca tggccgcgaa
     1681 cagccgccgt ggactaatgc ggcggcagca cgttggaatg cagccagaac cgcaccgtgt
     1741 gctcgaccac gcgacggaac tgcgagccgt ccagcggctt gatgacgaag gcgttcgcgc
     1801 cgagctggta gctcaggtag acctcggcct cgcgctgcga ggacgtgaag acgacgatcg
     1861 ggatgcggcg cgactgcgcg tggcggcgca cctcgcgcag cacctcgagc ccgtcgacca
     1921 gcggcagcac gagatcgagc aggatcagcg agggcgcagg cgccgcgcct tcgcgctcgt
     1981 agtccgtctc gcgcaggcgg gcgagcgcgg cctgcccgtt cacgacgacg gtcacggtgc
     2041 aatcgccgac ggactcgacg gcggacttgg tcatctcggc atcgagggcg tcgtcttcga
     2101 cgagcaggac ggacttgttc agcatggcgg acggcgatgg atggacgacc actgtagctg
     2161 cgaccgcgcc gcctgcctgt cagacggcat ccgatcgcgc aggtgcggac agcgcggcgc
     2221 gcaggtagcg gccggtcggc ccggcgccgt cgcgcgcgac ctcggccggc gtcccgcagg
     2281 caacgacgcg gccgcccgcc tcgcccgcgc ccggccccat gtcgacgatc cagtcggccg
     2341 ccgcgacgag gcgcatctcg tgctcgacga cgaccaccgt gttgccggcg tcgacgaggc
     2401 cctgcagctg cgccatcatg cggtcgacgt ccgcgggatg caggccggtg ctgggctcgt
     2461 cgaggacgta aagcgtgtcg ccgcgctgcg gccgctgcag ctcggtggcg agcttgatgc
     2521 gttgcgcctc gccgcccgac agctcggtgg ccggctgccc gaggcgcagg tagccgaggc
     2581 cgatgtcgcg cagcagcgcg agcggcctgc ggatcgcggg ctcgccgtcg aagacgtcga
     2641 cggcctcgtc gaccgtcagc gcgagcacgt cggcgatgtt gcggccgttc cagcgcactt
     2701 gcagcgtctc gtcgtcgtag cgcgcgccat ggcaggccgg gcacggcgcg tacacgctgg
     2761 gcatgaacag caactcgacg ccgacgaagc cctcgccctc gcaggtcgcg cagcgcccct
     2821 tggcgacgtt gaacgagaag cggccggcgt cgtagcgccg cttgcgcgcg gccggcgtgg
     2881 cggcgaacag cttgcgcacc gcgtcgaaca ggccggtgta ggtcgcgagg ttggagcgcg
     2941 gggtgcggcc gatcggcttc tggtcgacgc gcaccagccg ccgcacgtga tgcgcgccgg
     3001 catgcaggcg gccgcccgtc ggcacgacga cctccggcgc gccggcctcg ggctcgtcgt
     3061 cggcgtcctc cggctcgcgc cccaggtgcg cggccacgag ttcgaccagc gcctggctca
     3121 cgaggctgga cttgcccgag ccggacacac cggtcaccgc ggtgaagacg ccgagcggaa
     3181 agcgcacgtc gagcgcgcgc aggttgttgc gcgtgacgcc gcgcagctcg agccaggcct
     3241 taggcgcgcg cggcggccgc gtgcgcgcgt ccggcggctc gaacaggtac tgcgccgtgc
     3301 gcgaggcggc gacgccgcgc aggcctgccg gcggcccgct gtaaagcacc tcgcccccct
     3361 gcgtgccggc gcccgggccg acgtccacga tccagtcggc gcggcggatc gtgtcgaggt
     3421 cgtgctcgac gacgaacagc gagttgccgg ccgccttcag ccggtcgagc gcgcgcagca
     3481 gcgcctcgct gtcggccgga tgcaggcccg cggtcggctc gtcgagcacg tagacgacgc
     3541 cgaacaggtt cgagtggatc tgcgtcgcca gccgcagccg ctgcagctcg ccgggcgaga
     3601 gggtcggcgt ggcacgctcc atggacaggt accccaggcc gaggtccgcg agggtcgcga
     3661 tgcgctcgcg cagggcctgg gcgatgcgct gggcggcgag gcgcttctcc tgcgagaggt
     3721 tgggcgtgcg gcgcacgtcc ggcgccgcgg catgcgccga gcctcccgcc gcgacccggc
     3781 gcgcggtgtc gcggc
//
LOCUS       DASVLN010000380         4121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_450036, whole genome shotgun sequence.
ACCESSION   DASVLN010000380 DASVLN010000000
VERSION     DASVLN010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450036"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1174
                     /locus_tag="VF453_09300"
     CDS             176..1174
                     /locus_tag="VF453_09300"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX7687891.1"
                     /translation="MQADGLKRFYQVSRGLLAKPASVRALDGVSFTLHAGRTLAVVGE
                     SGCGKSTLARVLTMIEPPGAGTLRIGGMEVAGASPAERRQLRRDVQIVFQNPYGSLNP
                     RQTVGAALAEPLAVNTPMDAAERDAAVRAMMVRVGLRPEYHGRYPHMFSGGQRQRIAI
                     ARALMLRPKVLVLDEPVSALDVSIRAQVLNLLVDLQREFGLAYVFISHDLSVVRHVAD
                     DVLVMYLGRAVEHGTRAQVFAQPQHPYTRALLSATPVADPAARERRAQRRIILRGELP
                     SPFAPPPGCAFNPRCPIATPACREAVPPLLPEDAAMQRFACIAPAERRLDTSSWGA"
     gene            complement(1309..1548)
                     /locus_tag="VF453_09305"
     CDS             complement(1309..1548)
                     /locus_tag="VF453_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687892.1"
                     /translation="MRQAPNLANPFIMLTSPDTILAAVERSERLNRLSRKVYRPLDRP
                     LIPKVGARDTAEFDRAIDDAEIPDAELDEIEDEQA"
     gene            complement(1728..2399)
                     /locus_tag="VF453_09310"
     CDS             complement(1728..2399)
                     /locus_tag="VF453_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7687893.1"
                     /translation="MRPIQATSRPVLLLVLAAAFAATVPAYAKRQLPADARDEFFHDI
                     EIDYASGLGELLELGFDPNTRTDKGQPALSQALIGQSLKAAKALWESPRIEIDARNDA
                     DETPLMLAALKSEADACAALLARGAAVNKAGWSPLHYAATGGNAAIVRMLVAKGAAIE
                     ARSPNGTTPLMMAAMYGSEEAVDALLAAGASRSAKNDQGLDASAFAARAGRDRLAARL
                     KADAS"
     gene            complement(2476..3267)
                     /locus_tag="VF453_09315"
     CDS             complement(2476..3267)
                     /locus_tag="VF453_09315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7687894.1"
                     /translation="MYVDSHCHLSFPELHERLHAILEDMRAAEVGGALCICTAMEEFE
                     RVHALALDHDRLWASVGVHPDTEDCAEPDIEALCAAAARPRVIAIGETGLDYYRLEGR
                     SVADMAWQRERFRVHIRAGLRTGKPLVIHTRAASADTLAILREEGAGESRGVFHCFTE
                     TMEVARAALDLGFHISFSGIVTFRNAADLREIARYVPLDRCLIETDSPYLAPMPHRGK
                     VNTPAWVPHVAHLVAELKGLTHAEVADATRDNFERLFDVRLPELA"
     gene            complement(3433..3822)
                     /locus_tag="VF453_09320"
     CDS             complement(3433..3822)
                     /locus_tag="VF453_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7687895.1"
                     /translation="MVDSERPSVMPNPPPASARPSVIQLVFREKGALYAAYMPIFAEG
                     GLFVPTTRDYQLGDDIYLLLTLPGDPQRYPVAGKVGWITPANAPGGRTQGVGVRFPQD
                     EKTRALKLRIEEILGTSISSSKPTQTI"
BASE COUNT          530 a         1439 c         1553 g          599 t
ORIGIN      
        1 tcgtccccgc gcacgcggcg cccgagctcg gcgcggcgct gtgccactac ccgctgcggc
       61 gcggcgagcc tctgcgccat cccgccaccg aggcctcggc atgacgacgt tcggcaccga
      121 cctgcccgag ggggcgctcg ccgagcgggc gttgccgcgg gacgccccga tcgtgatgca
      181 ggcagacggc ctgaagcgct tctaccaggt gagccgcggc ctgctggcga agccggcgag
      241 cgtgcgggcg ctcgacggcg tctccttcac gctgcacgcg gggcgcacgc tggccgtcgt
      301 gggcgagtcg ggctgcggca agtcgacgct cgcgcgcgtg ctgacgatga tcgagccgcc
      361 cggcgccggc acgctgcgca tcggcggcat ggaggtcgcc ggcgcatcgc ccgccgagcg
      421 tcgccagctt cgccgcgacg tgcagatcgt gttccagaac ccgtacggct cgctgaatcc
      481 gcgccagacc gtcggcgcgg cgctcgcgga gccgctggcc gtcaacacgc cgatggatgc
      541 cgccgagcgc gacgccgcgg tgcgcgcgat gatggtgcgc gtcggcctgc gcccggagta
      601 ccacggccgc tacccgcaca tgttctcggg cggccagcgc cagcgcatcg ccatcgcgcg
      661 ggcgctgatg ctgcggccca aggtgctggt gctcgacgag cccgtgtcgg cgctggacgt
      721 ctcgatccgc gcccaggtgc tcaacctgct ggtcgacctg cagcgggaat tcgggctcgc
      781 gtacgtcttc atctcgcacg acctgtcggt ggtgcggcat gtcgccgacg acgtgctggt
      841 gatgtatctc ggccgcgcgg tcgagcacgg cacgcgcgcc caggtgttcg cgcagccgca
      901 gcatccgtac acgcgggcgc tgctgtcggc cacgccggtc gccgacccgg cggcgcgcga
      961 gcggcgcgcc cagcgtcgca tcatcctgcg gggcgagttg ccctcgccgt tcgcgccgcc
     1021 gcccggctgc gctttcaacc cgcgctgccc gatcgcgacg ccggcttgcc gcgaagccgt
     1081 gccgccgctg ctgcctgaag acgccgcgat gcagcgcttc gcctgcatcg cgccggccga
     1141 gcggcggctg gacacgtcga gctggggcgc ctgaagcgcg caggtcccgg ctcggctggc
     1201 aggtcggcga aaggccgtgc cgcacggtgg agcatcacgc gggccggtac gccggcctcg
     1261 cgggaaggaa ggacatgccg tgccgtgccg tcgccggccg gcgggacgtc aggcctgctc
     1321 gtcctcgatc tcgtcgagct cggcgtccgg gatctcggcg tcgtcgatcg cgcggtcgaa
     1381 ctcggcggtg tcgcgcgccc cgaccttcgg gatcagcggt cggtcgagcg gacggtagac
     1441 cttgcggctc aggcggttca ggcgttccga gcgctcgacc gcggccagga tggtgtccgg
     1501 cgaggtgagc atgatgaacg ggttcgcgag gttcggggct tggcgcatgg tgggctcctg
     1561 gatctctgga acagacccgg acatcaggcc tcgttccatg gagcgaactc taggctgcgc
     1621 gacctcggac ctgtagtcgg ccccgggcca tacgcgttgt cggacaattt cttacacatc
     1681 ggtcggcccg cggcgggaac ttgcggggaa atctcgacgg tcgcgcgtca gctcgcatcg
     1741 gctttgagcc gcgcggccag ccggtctcgc cccgcccggg cggcgaaggc cgaggcgtcg
     1801 aggccctggt cgttcttcgc gctgcggctc gcgccggcgg cgagcagcgc gtcgacggcc
     1861 tcctcgctgc cgtacatcgc cgccatcatc agcggcgtcg tgccgttggg cgagcgcgcc
     1921 tcgatcgccg cgcccttggc gacgagcatg cgcacgatgg cggcgttccc gccggtcgcc
     1981 gcgtagtgca gcggcgacca gcccgccttg ttgaccgccg caccgcgcgc gagcagcgcg
     2041 gcgcaggcgt ccgcctcgct cttgagcgcg gccagcatca gcggcgtctc atcggcgtcg
     2101 ttgcgggcat cgatctcgat gcgcggagat tcccacagcg ccttggccgc cttcagcgac
     2161 tggccgatca gcgcctgcga cagcgccggc tggcccttgt cggtgcgcgt gttcggatcg
     2221 aagccgagct ccagcagttc gccgaggccg gatgcgtagt cgatctcgat gtcgtggaag
     2281 aactcgtcgc gcgcgtcggc gggaagctgg cgtttcgcgt acgcgggcac ggtcgcggcg
     2341 aatgcggcgg cgaggacgag caggaggacg gggcgggaag tcgcttggat gggacgcatg
     2401 gctgggtgac gcggcggcgc gggtcggatg cgggctcgtg ggtaccggag tcgacgacga
     2461 tggcaggcgc gagcgtcagg cgagctccgg caagcgcacg tcgaaaagcc gctcgaagtt
     2521 gtcgcgcgtc gcgtcggcca cttcggcatg ggtcagtccc ttgagctccg cgaccaggtg
     2581 cgccacgtgc ggcacccagg ccggcgtgtt gaccttgccc cggtgcggca tgggcgcgag
     2641 gtaggggctg tcggtctcga tgaggcatcg atccagcggc acgtagcgcg cgatctcgcg
     2701 caggtcggcg gcgttgcgga acgtgacgat gcccgagaac gagatgtgga agccgagatc
     2761 cagcgccgcg cgcgcgacct ccatcgtctc cgtgaagcaa tgaaacacgc cgcgcgactc
     2821 gcccgcccct tcctcgcgca ggatcgccag cgtgtcggcg ctcgcggcgc gcgtgtggat
     2881 cacgagcggc ttgccggtgc gcaacccggc gcggatgtgg acgcggaagc gctcgcgctg
     2941 ccacgccatg tcggccacgc tgcgtccctc gagccggtag tagtcgagtc cggtctcgcc
     3001 gatggcgatc acgcgcgggc gcgcggccgc ggcgcacaag gcctcgatgt cgggctcggc
     3061 gcagtcctcg gtgtccgggt gcacgccgac gctggcccag agacggtcgt gatccagcgc
     3121 cagggcgtgg acgcgctcga attcctccat cgcggtgcag atgcacagcg cgccgcccac
     3181 ctcggccgcg cgcatgtcct cgaggatcgc atgcaggcgc tcgtgcagtt cggggaagga
     3241 caggtggcag tgggaatcga cgtacatggc gaggatggaa tcatggacgc gggccgcggg
     3301 cggaccggga ggctggacgg cggcgcgccc gagctgcacg ggtcgctgcg cccgtcgcgg
     3361 caagtcgccg gcccgcgagg cggcgcgtcg atccggcggc cgcggccccg agtccgcggg
     3421 cggtggcgga cgtcagatcg tctgcgtcgg cttcgacgac gagatcgagg tgccgaggat
     3481 ctcctcgatg cgcagcttca gggcgcgcgt cttctcgtcc tgagggaagc ggacaccgac
     3541 gccctgggtt cgcccgcccg gcgcgttggc cggcgtgatc cagccgacct tgccggcgac
     3601 gggatagcgc tgcggatcgc cgggcagcgt cagcaggagg tagatgtcgt cgccgagctg
     3661 gtagtcgcgc gtcgtcggca cgaacaggcc cccttccgcg aagatgggca tgtaggccgc
     3721 gtacagcgcg cccttctcgc gaaagacgag ctggatgacg ctgggccgcg ccgaggccgg
     3781 cggcgggttg ggcatcacgg agggacgttc ggaatcgacc atgcgggcta gtgtagccac
     3841 tcggcggcgg cttgccgccg cgtgaacgcg gcaatttccg gctcgttttt cgcaggcgac
     3901 gcccggcgcg caccgccggg ccggttcgcg cgcgcagccg ccggcagcct ccggccgagc
     3961 gagggccttc agccgcgccg gccgcgcacc ggcgtccaga ccgcgcgcgc ctcggccagc
     4021 aggctctcga ccatcagcgg cgcgttgaac gggtgctcgt cgtgacgcgc gagccgggcg
     4081 agcgactgcg accagtcggc gagcctcgtc cacggcgccg c
//
LOCUS       DASVLN010000381         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_288902, whole genome shotgun sequence.
ACCESSION   DASVLN010000381 DASVLN010000000
VERSION     DASVLN010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288902"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VF453_09325"
     CDS             <1..536
                     /locus_tag="VF453_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HEX7687896.1"
                     /translation="ETFTLTMKEVALRSAAAPDAPAGAAPTLVTRPCAVGFSGNYPRA
                     LDGLARLLSLDMRVIDPAWIGMKLRKLLNVGEPLGHFMAPVPGQRRQQVWPSTVAYVA
                     RLMIHRYAMLGVLDEDGFPLRAMGLLAPPAAASSAAVPALRPQAGRPCPECGNATMIH
                     KDGCDFCTSCGYVGACG"
     gene            649..1830
                     /locus_tag="VF453_09330"
     CDS             649..1830
                     /locus_tag="VF453_09330"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX7687897.1"
                     /translation="MAGETKVEFVAGAEIELAFDPTLCIHSRFCVLQAPAVFQANTPG
                     AWIRPDEMSAPALAAVARNCPSGAIAYRATVAVLDEAAPPVNTIRLRQDGPYAVNAAI
                     RIVGHERAQPVHRRTLCRCGASRNKPFCDGSHAAIGFEATGEPPTDPAIRALDPRDGP
                     LVVTPMPDGPLEIAGPAELVSGTGRTIAKTAHALLCRCGGSRAKPFCDGTHSLNGFTD
                     RADGPHAPAPAADGGAQVPTLAEWAGGPEAFQALAAAFYAKVPADPVLAHVFADMDRR
                     HVERVGDFLAEVLGGPPAYSRQGGSHVGMIARHLGRHITEAQRARWVELLVETAAEIG
                     LPADAAFRAAFASYLEWGSKLAVINSAPGLAKPEGDWPMPAWGWGPAGGPALADPPPE
                     P"
BASE COUNT          226 a          734 c          716 g          251 t
ORIGIN      
        1 gcgagacctt cacgctgacg atgaaggagg tcgccctgcg ctcggccgcc gcgcccgacg
       61 cgcccgcggg cgccgcgccc acgctcgtca cccggccgtg cgcggtcgga ttctcgggca
      121 actacccgcg cgcgctcgac ggcctggcgc gcctgctgtc gctggacatg cgcgtgatcg
      181 accccgcctg gatcggcatg aagctgcgca agctgctcaa cgtcggcgag ccgctcggcc
      241 acttcatggc gccggtgccc ggccagcggc gccagcaggt gtggccgtcg acggtcgcct
      301 acgtcgcgcg gctgatgatc caccgctacg cgatgctggg cgtgctcgac gaggacggct
      361 tcccgctgcg cgcgatggga ctgctcgcgc cgcccgcggc ggcgtcgtcg gcggccgtgc
      421 ccgcgctgcg tccgcaggcc ggccgcccgt gccccgagtg cggcaacgcg acgatgatcc
      481 acaaggacgg gtgcgacttc tgcacgtcct gcggctacgt gggggcgtgc ggctgacgct
      541 gcgcggggcc gccctcgcgc aagccggcac gcaccgaccg gtgcgttgat ttcgccccgc
      601 gcctgtcgca cattgccgca gccgggcgga tcccggggag tgggcggcat ggcgggcgag
      661 acgaaggtgg agttcgtggc gggcgcggag atcgagctcg cgttcgaccc gacgctgtgc
      721 atccattcgc gcttctgcgt gctgcaggcg ccggccgtct tccaggccaa cacgccgggc
      781 gcttggatcc ggcccgacga gatgagcgcg ccggcgctgg cggcggtcgc gcgcaactgt
      841 ccctccggtg ccatcgccta tcgcgcgacg gtcgccgtgc tcgacgaggc cgcgccgccc
      901 gtcaacacga tccggctgcg gcaggatggg ccgtacgccg tcaacgcggc gatccggatc
      961 gtcggccacg agcgcgcgca gcccgtccat cgccgcacgc tgtgccgctg cggcgcgtcg
     1021 aggaacaagc cgttctgcga cggctcgcac gccgccatcg gcttcgaggc gaccggcgag
     1081 ccgccgaccg atcccgcgat ccgggcgctc gatccgcgcg acgggccgct cgtcgtcacg
     1141 ccgatgcccg acgggccgct cgagatcgcc ggcccggccg agctggtgag cggcaccggc
     1201 cgcaccatcg ccaagaccgc ccacgcgctg ctgtgccgct gtggcggctc gcgcgccaag
     1261 ccgttctgcg acggcacgca ctcgctcaac ggcttcaccg atcgcgcgga cggcccgcac
     1321 gcgccggcac cggctgcgga cggcggcgcg caggtgccga cgctggccga atgggcgggc
     1381 ggcccggagg ccttccaggc gctggccgcc gccttctacg ccaaggtgcc ggccgatccg
     1441 gtgctcgcgc acgtcttcgc ggacatggat cgccgccacg tggagcgcgt cggcgacttc
     1501 ctggccgagg tgctgggcgg cccgcctgcg tattcgcggc agggcggcag ccacgtcggg
     1561 atgatcgccc ggcatctcgg gcgccacatc accgaggcgc agcgcgcgcg ctgggtcgag
     1621 ctgctcgtgg agacggccgc cgagatcggc ctgccggcgg acgccgcgtt ccgcgcggcc
     1681 ttcgcctcgt atctcgagtg gggttcgaag ctcgcggtga tcaactccgc gcccggcctc
     1741 gcgaagcccg agggcgactg gccgatgccc gcgtggggct ggggtccggc cggcggaccg
     1801 gcgctggcgg atcccccgcc ggagccgtga cggcgcgcgc ctcaggcgct cgcggccgca
     1861 agcgccgcgg cgaaggcgtc gacctcgccc agcaccgcct cgccgacgaa gcgcatggtc
     1921 tcgagcg
//
LOCUS       DASVLN010000382         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_504282, whole genome shotgun sequence.
ACCESSION   DASVLN010000382 DASVLN010000000
VERSION     DASVLN010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504282"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VF453_09335"
     CDS             complement(<1..889)
                     /locus_tag="VF453_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7687898.1"
                     /translation="MALPPDQTSMALFCDFENVALGVRETQHEKFDIKLVLERLLVKG
                     SIVVKKAYCDWERYRSFKGPMHENNFELIEIPHVRQSGKNSADIRMVVDALDLCYTKS
                     HVNTFVIISGDSDFSPLVSKLRENAKQVIGVGVKQSTSDLLTANCDEFIFYDDLVRSS
                     RRGNPPPVHEGKKGPTPEEERKREETLKRRAKGVELAVETLEALISERGDGSKIWSSV
                     LKEAIKRRRPDFNESYFGFRGFSHLLEDAQARGLLELARDEKSNVYITRTSTPVPVPA
                     DAAGAQAAASAPAAGADVLP"
     gene            complement(924..>2120)
                     /locus_tag="VF453_09340"
     CDS             complement(924..>2120)
                     /locus_tag="VF453_09340"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX7687899.1"
                     /translation="LRLVAVATRTPAKARPLLPEGCRLGDDLAAVTDAADVDVVVELI
                     GGVEAARAAVLRAIAAGKHVVTANKALLARHGDEIFERAAARGVMVAYEAAVAVAIPI
                     IKLLREAQAASRIDALAGIVNGTSNFVLTRMREDGVDYADAVADAQARGFAEADPSFD
                     VDGNDAAHKLAILAALAYGIPMSLEAVRMRGIAGLSIRDVRFAEKLGHRIKLLAVARR
                     HDAGLELRVEPMLVPHAHMMAAVNAEMNAILVRGNASGTTLHYGAGAGGRPTASAVVA
                     DLVDVARLHGADPRHHVPVAAWHPASVRASAFVDPATLPAAHYLRVDVADEVGVLASV
                     TAALAAHGVSIDVLHQEAAHGGRTDLVALTHPCTERTLAEALADLRGLAAVSARVVVL
                     PVEPLF"
BASE COUNT          281 a          727 c          803 g          309 t
ORIGIN      
        1 gaggcagcac gtcggcaccg gccgccggcg ccgacgcggc cgcctgagcg ccggcggcgt
       61 cggccgggac gggcaccggc gtcgacgtgc gcgtgatgta gacgttggac ttctcgtcgc
      121 gcgccagctc cagcagcccg cgcgcctgcg cgtcctccag gaggtggctg aagccgcgga
      181 agccgaagta cgactcgttg aagtccggcc gccggcgctt gatcgcctcc ttgagcaccg
      241 aggaccagat cttcgagccg tcgccgcgct cggagatcag cgcctccagc gtctcgaccg
      301 ccagctcgac gcccttggcg cggcgcttga gcgtctcctc gcgcttgcgt tcctcctcgg
      361 gcgtcgggcc cttcttgcct tcgtgcaccg gcggcgggtt gcctcggcgc gacgagcgca
      421 cgaggtcgtc gtagaagatg aactcgtcgc agttggcggt cagcaggtcg gaagtcgact
      481 gcttcacgcc gacgccgatc acctgcttgg cgttctcgcg cagcttcgag acgagcggcg
      541 agaagtcgga gtcgccgctg atgatgacga aggtgttgac gtgcgacttc gtgtagcaga
      601 ggtccagcgc gtcgaccacc atgcggatgt cggccgagtt cttgccggac tggcgcacgt
      661 gcgggatctc gatcagctcg aagttgttct cgtgcatcgg gcccttgaag ctgcggtagc
      721 gctcccagtc gcaataggcc ttcttcacga cgatgctgcc cttgacgagc aggcgctcga
      781 ggacgagctt gatgtcgaac ttctcgtgct gcgtctcgcg cacgcccagg gcgacgttct
      841 cgaagtcgca gaacagcgcc atgctggtct ggtcgggggg cagggccatc ggggctcttt
      901 cttgggagga gtcgtcgggc cgctcagaac agcggctcga ccggcagcac caccacgcgc
      961 gcggagaccg cggccaggcc gcgcaggtcg gccagggctt cggcgagcgt gcgctcggtg
     1021 cagggatggg tgagcgcgac caggtcggtg cggccgccgt gcgcggcctc ctggtgcagg
     1081 acgtcgatcg acacgccatg cgcggcgagc gcggcggtga ccgaggcgag gacgccgacc
     1141 tcgtcggcga cgtcgacgcg caggtagtgc gccgcgggca gcgtggccgg atcgacgaag
     1201 gccgacgcgc gcaccgacgc gggatgccac gccgccaccg ggacgtggtg gcgcgggtcg
     1261 gcgccgtgca ggcgggcgac gtcgacgagg tcggcgacga ccgccgaggc cgtcggccgt
     1321 ccgcccgcgc cggcgccgta gtgcagcgtg gtgccggagg cgttcccgcg cacgaggatg
     1381 gcgttcatct cggcgttgac cgcggccatc atgtgcgcgt gcggcacgag catgggctcg
     1441 acgcgcagct ccagcccggc gtcgtggcgg cgggcgacgg cgagcagctt gatgcggtgg
     1501 ccgagcttct cggcgaaccg cacgtcgcgg atggacagcc cggcgatgcc gcgcatgcgc
     1561 acggcctcca ggctcatcgg gatgccgtag gcgagcgccg cgaggatcgc cagcttgtgc
     1621 gccgcgtcgt tgccgtccac gtcgaaggac gggtcggcct ccgcgaagcc gcgcgcctgc
     1681 gcgtcggcga ccgcgtccgc gtagtccacg ccgtcctcgc gcatgcgcgt cagcacgaag
     1741 ttggacgtgc cgttgacgat gccggcaagc gcgtcgatgc ggctggcggc ctgcgcctcg
     1801 cgcagcagct tgatgatggg gatcgcgacg gcgacggcgg cctcgtaggc gaccatcacg
     1861 ccgcgcgcgg cggcgcgctc gaagatctcg tcgccgtggc gcgcgagcag cgccttgttg
     1921 gcggtgacga cgtgcttgcc ggccgcgatc gcgcgcagca cggcggcgcg cgcggcctcg
     1981 acgccgccga tcagctcgac caccacgtcg acgtcggcgg cgtcggtgac ggcggcgagg
     2041 tcgtcgccca ggcggcagcc ctcgggcagc agcggccgcg ccttcgccgg cgtgcgcgtg
     2101 gccacggcga ccaggcgcag
//
LOCUS       DASVLN010000383         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_395374, whole genome shotgun sequence.
ACCESSION   DASVLN010000383 DASVLN010000000
VERSION     DASVLN010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395374"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VF453_09345"
     CDS             <1..656
                     /locus_tag="VF453_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7687900.1"
                     /translation="VAFGRAWLAGAPRVFVALGRHNALLLKLVLAGGEPPQACWRGTS
                     ALHMAASAGYVDDVELLLAAGARPDGLLDDAGGSPLLTALSAGHYGIAETLLAHGADP
                     RIVADGGRTGLHALALPLVAPGQALRADVPARQRALAERLIVAGVPVDARMTVRRTTP
                     LMIAASAGDGEMVQLLLDHGADPGLRNVDGLSAADLAEHRSHPGLAALLRAWLGPAR"
     gene            689..1600
                     /locus_tag="VF453_09350"
     CDS             689..1600
                     /locus_tag="VF453_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7687901.1"
                     /translation="MTDSQFQGRTAVITGAASGFGLETSLLAARLGMNVVMADVMAEP
                     LAAAAREVEALGAAVLALRTDVRAAADVEALAARTVERFGVPHLVFNNAGVGAGGLVW
                     EHTAADWEWVMGVNVMGVAHGVRVFAPLMLGAEKADPAYRGHIVNTASMAGLVNMPNM
                     GVYNVSKHAVVSLSETLYQDLALVSERVRAHVLCPYFVPTGIARSERARADEAAAGAT
                     PTPSQRIARASVERAVASGKVSAAEVAQFVFDALVADRFYIYSHPKALAPVATRAEDI
                     VQGRNPTDPFRERPELGAQLRAALQGG"
     gene            1840..2049
                     /locus_tag="VF453_09355"
     CDS             1840..2049
                     /locus_tag="VF453_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7687902.1"
                     /translation="MATETGIVKWFNDGKGFGFITPDSGGKDLFAHFSEIQATGFKSL
                     AENQRVEFEVKQGEKGLQAANIRAL"
     gene            2497..2733
                     /gene="infA"
                     /locus_tag="VF453_09360"
     CDS             2497..2733
                     /gene="infA"
                     /locus_tag="VF453_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX7687903.1"
                     /translation="MAKEELIEMHGVVEDVLPDSRYRVKLDNGHQLVAYTSGKMRKHH
                     IRILAGDKVSLELSPYDLSKGRITFRHIERRGPV"
     gene            complement(2873..3457)
                     /locus_tag="VF453_09365"
     CDS             complement(2873..3457)
                     /locus_tag="VF453_09365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7687904.1"
                     /translation="MSFTPPTTTDRLAPEAAEAVLQRWIADENAKRERMERAGPGVAS
                     PAQLAGRTGLQQLQAMLDGELPYAPIARTLDFALIEVGPGMAVFQGSPGPQHFNPMGG
                     VHGGWFATLLDSALGCAVHTMMPVGRGYTTAELGVNYVRALTPKPGRVRAEGRVVHCG
                     KQLATAEARLVGPDGALYAHATTTCLCFELPAAR"
     gene            3528..>3917
                     /locus_tag="VF453_09370"
     CDS             3528..>3917
                     /locus_tag="VF453_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7687905.1"
                     /translation="MKSPTAPEAASMPARPYGCTNLKLRQLMRRVARHYDAEMGKAGL
                     KGTQYSLLSHVVKLGPIRPVDLAATMRVDASTLSRNLKPLVAAGWLALEAGEDGRSRL
                     VHATDAGRDKRAEAQRRWRVAQERLNEA"
BASE COUNT          539 a         1419 c         1405 g          554 t
ORIGIN      
        1 tggtcgcctt cgggcgcgcc tggctggccg gcgcgccgcg cgtgttcgtg gcgctgggcc
       61 ggcacaacgc gctgctgctg aagctcgtcc tcgcgggcgg ggagccgccg caggcctgct
      121 ggcgcggcac gagcgcgctg cacatggccg cgtccgccgg ctatgtcgac gacgtcgagc
      181 tcctgctcgc ggcgggcgcg cggcccgacg ggctgctcga cgacgcgggc gggtcgccgc
      241 tgctgacggc gctctcggcg ggccactacg gcatcgccga gacgctgctg gcgcacggcg
      301 cggatccgcg catcgtcgcc gacggcggcc gcaccggcct gcacgcgctg gcgcttcccc
      361 tcgtggcgcc ggggcaggcc ttgcgggcgg atgtgccggc acgccagcgc gcgctcgccg
      421 agcgcctgat cgtcgccggc gtgccggtcg acgcccggat gacggtgcgc cgcacgacgc
      481 cgctgatgat cgccgccagc gccggcgacg gcgagatggt gcagctgctg ctcgatcacg
      541 gcgccgaccc gggcctgcgc aacgtcgacg gactgtcggc ggccgacctc gccgaacatc
      601 gatcccatcc cgggctcgcc gctctgctgc gggcgtggct cggcccggcg cgttgagcgc
      661 cacgtcctcc gcccccagct tccacgccat gaccgattcc cagttccagg gccgcaccgc
      721 ggtcatcacc ggcgccgcct ccggcttcgg cctcgagacc tcgctcctgg ccgcgcggct
      781 gggcatgaac gtcgtcatgg ccgacgtgat ggccgagccg ctcgcggccg cggcgcgcga
      841 ggtcgaggcg ctcggcgccg cggtgctcgc gctgcgcacg gacgtgcgcg cggccgccga
      901 cgtcgaggcc ctggccgcgc gcaccgtcga gcgcttcggc gtgccgcacc tggtcttcaa
      961 caacgcgggc gtgggcgccg gcggcctggt ctgggagcac accgccgccg actgggaatg
     1021 ggtgatgggc gtcaacgtga tgggcgtcgc gcacggcgtg cgcgtgttcg cgccgctgat
     1081 gctgggcgcc gagaaggccg acccggccta ccgcggccac atcgtcaaca ccgcctccat
     1141 ggcggggctc gtgaacatgc ccaacatggg cgtctacaac gtgagcaagc acgcggtggt
     1201 gtcgctcagc gagacgctct accaggacct cgcgctggtc agcgagcgcg tgcgcgccca
     1261 cgtgctgtgc ccgtacttcg tgcccaccgg catcgcccgc agcgagcggg cgcgcgcgga
     1321 tgaggccgct gccggggcga cgccgacgcc gagccagcgc atcgcccggg caagcgtcga
     1381 gcgcgcggtg gccagcggca aggtgagcgc ggcggaggtg gcgcagttcg tcttcgatgc
     1441 gctcgtcgcc gaccgcttct acatctacag ccatccgaag gcgctcgcgc cggtggccac
     1501 gcgggccgag gacatcgtgc agggccgcaa tccgaccgac ccgttccgcg agcggcccga
     1561 gcttggcgcg cagctgcgcg cggcgcttca aggcggctga atgcgccgtc gcgcggcggc
     1621 gttgtcaggg ttcgtccggg accgccgcac cggggcggcg gcgcctgctc tgctatcatg
     1681 acggagtagg caatacctaa gacggtcgtc cgaaggactc aaccgccgcc cgagccgttc
     1741 caggccgcgc gtggtcgatc tctcccgtga ttttcttgcg tgtcacctgc cctgcgctgg
     1801 catgtgctgc gcttgatctt cgaaaacagg atgtgagata tggcgaccga aaccggtatc
     1861 gtgaagtggt tcaatgacgg caagggcttc gggttcatca ccccggactc cggcggcaag
     1921 gacctcttcg cgcacttcag cgagatccag gccacgggct tcaagagcct ggccgagaac
     1981 cagcgcgtcg aattcgaggt gaagcagggc gaaaagggcc tgcaagccgc gaacatccgc
     2041 gcgctgtgac gctgccgggc cgcgccgccg cgaaggcggc cgcggccctt gttttttgcg
     2101 cggccgacgc caactcaggc gcttcggcga ccgcccggcc tgtcgcgcgc aagcgtcgag
     2161 ggccccacgc ccgggcgccg aaagccttaa aataggcaga gttcgagtcc ggcgagtacg
     2221 cggattgccc cggtggcgat accgcgtgcc ccaggtggat ccgccgatcc gccgccggtt
     2281 ccagcggtcg gacgtcgcgg caacgcgatg gccggccccg cgcgatgcgc gtcgaccgct
     2341 ttcgatgcga aagcgtccgc gacgccgccg cgccacgcca ctttcgacgg tcctgaaacg
     2401 ggatgcgccc gccgccggcg cgcgcccttc tgagcgggtc ctggacgcac gtggcgccgc
     2461 aggttttgac atcgcaagga ttcaggagcg ttttcaatgg cgaaagaaga attgatcgaa
     2521 atgcatggcg tcgtcgaaga cgtcctgccc gactcgcgct accgcgtgaa gctggacaac
     2581 ggccaccagc tggtcgccta cacgtccggc aagatgcgca agcaccacat ccgcatcctc
     2641 gcgggcgaca aggtgtcgct cgagctgtcg ccctacgacc tcagcaaggg ccgcatcacc
     2701 ttccgtcaca tcgagcggcg cggtccggtc tgagcgccgg ctccccgctc gtcgtcctcc
     2761 ttgcgagacc cgcggcctgg cccgcgggtt tttcgcttgt gcggcgcagg ttcgtcgccc
     2821 gccgccccct tgcgcgtgag gcgcggggtg aagcagcccg ccgcgccgtg tctcaacggg
     2881 ccgccggcag ctcgaagcac aggcaggtcg tcgtagcgtg cgcgtagagc gcgccatccg
     2941 ggccgaccag ccgggcctcc gccgtcgcca gctgcttgcc gcagtggacg acgcgcccct
     3001 cggcgcgcac gcggcccggt ttcggcgtga gcgcgcgcac gtagttcacg cccagctccg
     3061 cggtggtgta gccacgaccc acgggcatca tcgtgtggac ggcgcagccg agcgccgagt
     3121 ccagcagcgt cgcgaaccag ccgccgtgca cgcctcccat cgggttgaag tgctgcggcc
     3181 cgggcgagcc ctggaacacg gccatccccg gcccgacctc gatcagcgcg aagtccagcg
     3241 tgcgggcgat cggcgcgtac ggcagctcgc cgtccagcat cgcctgcagc tgctgcagcc
     3301 cggtccggcc cgccagctgg gcgggactcg cgacgccggg gccggcgcgc tccatgcgct
     3361 cgcgcttggc gttctcgtcg gcgatccatc gctgcaggac ggcctcggcg gcctccggcg
     3421 caaggcggtc ggtggtggtg ggcggggtga agctcatggc ggatcctggt cgggacgggg
     3481 tgaatgaatg catatgcaat caatgtagct gcaactataa tcgctccatg aaatcaccca
     3541 ccgcgccgga ggcggcatcc atgccggcgc gcccctacgg ctgcaccaac ctgaagctgc
     3601 gccagctgat gcggcgcgtc gcgcggcact acgacgcgga gatgggcaag gcggggttga
     3661 agggcacgca gtactcgctg ctgagccacg tggtcaagct cgggccgatc cggccggtcg
     3721 acctggccgc gacgatgcgc gtcgatgcct cgaccctgtc gcgcaacctg aagccgctgg
     3781 tggccgccgg ctggctggcg ctggaggccg gcgaggacgg ccgcagccgc ctcgtccacg
     3841 cgaccgacgc cggccgcgac aagcgcgccg aggcgcagcg ccgctggcgc gtcgcgcagg
     3901 agcggctcaa cgaggcg
//
LOCUS       DASVLN010000384         5632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_20053, whole genome shotgun sequence.
ACCESSION   DASVLN010000384 DASVLN010000000
VERSION     DASVLN010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20053"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..872)
                     /locus_tag="VF453_09375"
     CDS             complement(138..872)
                     /locus_tag="VF453_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7687906.1"
                     /translation="MAFCCLCERAVPAWVPYPRAAQRSQFMILMETVGSDLSVYGCPH
                     CGCNDRDRHLWLYMLAAGLPAQLAGARVLHLAPERALEPRLRACGLAEYVRGDLFPAR
                     EGIVKVDAEAMPFEDGRFDLVIANHLLEHVHHPERALAEFERCLAPGGMVIAQTPYAP
                     RLKHTFELDARPTPDFAAMFYGQDDHVRLFGGDIARYFHDAGLRGALLPHEQLLADID
                     PLEFGCNGLEPFFAFSKPEAATAEAA"
     gene            complement(884..2422)
                     /locus_tag="VF453_09380"
     CDS             complement(884..2422)
                     /locus_tag="VF453_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7687907.1"
                     /translation="MTTVPAIHLAIIQPAGYVHSLGFLDQARYFRHQFRRLGAKVTMA
                     KNRLREDAVNFVFGAHLGFPAEWQQRHPCVFVNLEQLGQGGAAVSADYLALLRRSAVV
                     DYDAANIPAYAADPADVPVVSFGYASYLDQPALPLEQRPIDLLFFGSMNERRRSFIER
                     VEAAGFAVAAFDHPIYGPERDAYIAQAKAVLNCHFYESNRFEQARAFHCLSLGTPVIS
                     ERTPSTAPSAAFEDSVLWLPEDAQSWFAQHFATPGFYDAARECLAAFREAGPAHDPID
                     AYAEVLAFAAGFSQGFDEQLRAPGPWRPRAINLGSGKDYKPNWLNIDILERAQPDLVL
                     DLGREVAFPHEAPTLMGGTVVLEAGSVDVIYANNVLEHVPDLPRLMGNALALLREGGE
                     FQIEVPYEKALTAWQDPTHLRALNENSWVYYTDWFWYLGWFEHRFEMTQSAWLDANVQ
                     PCAKDKAAFMRVTLRKIATTPRERTVARTMRADFGGLDDDLPFAADAPAPATARATPV
                     PTAH"
     gene            2712..3317
                     /locus_tag="VF453_09385"
     CDS             2712..3317
                     /locus_tag="VF453_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7687908.1"
                     /translation="MIKHFALAAILAAASAAASAAGPNLLLDGDFESTPVAAGGWTTV
                     SATPGWTSTTHGIELRNDAVGVAESGVNFVELDTDQNSSMYQTVTLAAGEYTLSFWVQ
                     DRAGTDAATNGLSVSVGGLLPTQVLAGGAYPEWTKVTETFDVGTATTTTLTFAAAGVS
                     DSLGTSLDNVSLTAVPEPSGLVLAASGLALLGLARRRARKA"
     gene            complement(3416..4483)
                     /locus_tag="VF453_09390"
     CDS             complement(3416..4483)
                     /locus_tag="VF453_09390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7687909.1"
                     /translation="MAAYRVALVMIARNEAARIVRALASARNCVDELVVVDTGSTDAT
                     VALARSAGARVAHFAWVDDFSAARNAALALADADWNVVVDADEWIAEHGLELLALRHT
                     APEFVGALRVDSQFDTPLGISVAPSWLPRVLPRGVRYEGRVHEQPRHALPVRQLATTL
                     AHDGYRAQALQAKSGRNAGLLERALAQSPDDAYLHYQLGKDHDVYERYVEAASHFARS
                     RGCEASHGAGGVTLVGGVPGWHHDRLVREIHALKRCRRHGEAVQLAEASMAAWADSPD
                     FFFALGDLLLDWATEEPARAGELLPMIEQAWLRCLEIGERPELEGSVAGRGSSLAARN
                     LAVLYEGTGRAIEALRFRQMT"
     gene            complement(4486..>5632)
                     /locus_tag="VF453_09395"
     CDS             complement(4486..>5632)
                     /locus_tag="VF453_09395"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687910.1"
                     /translation="PLPPPGPRKPGRIRIGYLSADFYQHATAVLMAELLERRDRERFE
                     VFLYSHSPDDGHDVTRRVRAACEHFVDVNDLGHKAIAQRMRDDGIDIAVDLKGHTRDS
                     RMDLLAYRPAPVQASYLGYPASSGADFIDYFIGDRITTPLSHAANYSEHIAQLAGCYQ
                     PNDASRALPPCPPRAELGLPEDAVVLCCFNQTYKLSPHMLDLWAAILAGAPRAVLWML
                     AWNPHAKENLLRELAARGVPSERVIFAPKLGLAGHIARLRAADLFLDTWPCNAHTTAS
                     EALWAGVPVLTVPGPTFASRVAASLVASCGLGELACADAAHYVAFATALANEPQTLQG
                     LKQHLETHRRALPLFDAERLARDLDALYLRMHERHLAGLPPAALAAE"
BASE COUNT          788 a         1919 c         2134 g          791 t
ORIGIN      
        1 gcggaggtac gtcaatcggg catgcttatg ggtgttcatc cggttccggc tttgaagttg
       61 acggggggtt tggcgattcc cagtctctca aaacctctcc gggtgaacac ccgcaacaac
      121 ctattgaacc ttcacaccta ggcagcctcg gccgtcgccg cctcgggctt cgagaacgcg
      181 aagaagggct ccagcccgtt gcagccgaac tcgagcggat cgatgtccgc gagcagctgc
      241 tcgtgcggca gcagcgcgcc ccgcaggccg gcgtcgtgga agtagcgcgc gatgtcgccg
      301 ccgaacaggc gcacgtggtc gtcctggccg tagaacatcg ctgcgaagtc cggcgtcggc
      361 cgggcgtcga gctcgaaggt gtgtttcagc ctgggcgcgt acggcgtctg cgcgatgacc
      421 atgccgcccg gcgccaggca gcgctcgaac tccgcgagcg cacgctcggg atggtggacg
      481 tgctcgagga ggtggttggc gatgacgagg tcgaagcggc cgtcctcgaa gggcatcgcc
      541 tccgcgtcga ccttgacgat gccctcgcgc gcggggaaaa ggtcgccgcg cacgtactcc
      601 gcgagcccgc aggcgcgcag gcgcggctcg agcgcgcgtt ccggcgccag gtgcaggacg
      661 cgcgcgccgg ccagctgcgc gggcaggccg gccgccagca tgtagagcca caggtggcgg
      721 tcgcggtcgt tgcagccgca gtgcgggcag ccgtagaccg acaggtcgga gcccacggtc
      781 tccatcagga tcatgaactg gctgcgctgg gcggcgcggg ggtagggcac ccaggcggga
      841 acggcgcgtt cgcagaggca gcagaaggcc atggcgacga ctcctagtgg gccgtgggaa
      901 cgggcgtggc gcgggccgtg gccggcgccg gggcgtcggc ggcgaagggc aggtcgtcgt
      961 ccaggccgcc gaagtcggcg cgcatcgtgc gggcgacggt gcgctcgcgc ggcgtcgtgg
     1021 cgatcttgcg cagcgtcacg cgcatgaagg ccgccttgtc cttcgcgcag ggctgcacgt
     1081 tcgcgtccag ccaggcggac tgcgtcatct cgaagcggtg ctcgaaccag ccgaggtacc
     1141 agaaccagtc ggtgtagtag acccacgagt tctcgttgag cgcgcgcagg tgcgtcggat
     1201 cctgccaggc ggtgagcgcc ttctcgtagg gcacctcgat ctggaactcg ccgccctcgc
     1261 gcagcagcgc cagcgcgttg cccatcagcc ggggcaggtc gggcacgtgc tccagcacgt
     1321 tgttggcgta gatgacgtcc acgctgccag cctccagcac gaccgtaccg cccatcagcg
     1381 tcggcgcctc gtgcgggaac gcgacctcgc ggccgaggtc gagcacgagg tcgggctggg
     1441 cgcgctcgag gatgtcgatg ttgagccagt tcggcttgta gtccttgccc gagccgaggt
     1501 tgatcgcgcg cggccgccac gggcccggcg cgcgcagctg ctcgtcgaaa ccctgcgaga
     1561 agccggccgc gaaggcgagc acctcggcat aggcgtcgat cgggtcgtgc gccggccctg
     1621 cctcgcggaa ggcggcgagg cactcgcgcg cggcgtcgta gaacccgggc gtcgcgaagt
     1681 gctgcgcgaa ccaggactgc gcgtcctcgg gcagccacag cacgctgtcc tcgaaggcgg
     1741 ccgagggcgc ggtcgagggg gtgcgctcgg agatcaccgg cgtgcccagc gacaggcagt
     1801 ggaacgcgcg ggcctgctcg aagcggttgc tctcgtagaa gtggcagttc agcaccgcct
     1861 tggcctgggc gatgtaggcg tcgcgctcgg ggccgtagat cggatggtcg aaggcggcga
     1921 ccgcgaagcc ggcggcctcc acgcgctcga tgaagctgcg gcggcgctcg ttcatgctgc
     1981 cgaagaacag caggtcgatc gggcgctgct ccagcggcag ggcgggctgg tccaggtagg
     2041 acgcgtagcc gaacgacacg acgggcacgt cggccggatc ggcggcgtac gcggggatgt
     2101 tggcggcgtc gtagtcgacc accgccgacc ggcgcaacag ggcgaggtag tcggcgctca
     2161 cggcggcgcc gccttggccc agctgctcca ggttgacgaa cacgcaggga tggcgttgct
     2221 gccactctgc cgggaagccc aggtgcgcgc cgaagacgaa gttgaccgcg tcctcgcgca
     2281 gccggttctt ggccatcgtc accttcgcgc cgaggcggcg gaactggtgc cggaagtagc
     2341 gcgcctggtc gaggaagccg agcgagtgga catagccggc cggctggatg atggccaggt
     2401 ggatcgcggg gacggtcgtc attgaggggc gctccgggtc ggttctcgcg gccgcggtgg
     2461 ccgcggatgc ggcgcgcacg ggcggcgatg ggtcgactgt agtgagcgca ccgccggggc
     2521 gacggcgcga aaagcggggt gtccctacgg catttcgcag cgcgccttga gcgccgggcg
     2581 tctctttttc ggcgctgccc ggccgtttat cgggcaatgt gtcacgaatt gcaaccgcga
     2641 ccgcttccgc tgcgcggccg ggctcgtcaa aattggtgct atccctgacg ccgatccgat
     2701 tgaacccgac catgatcaag cacttcgccc tcgccgccat cctcgctgcc gcgtccgccg
     2761 ccgcctccgc cgcgggcccg aacctgctgc tggacggcga cttcgagtcc accccggtcg
     2821 ccgccggcgg ctggaccacc gtcagcgcca ccccgggctg gacgtcgacg acccacggca
     2881 tcgagctgcg caacgacgcc gtcggcgtcg ctgaatcggg cgtcaacttc gtcgagctgg
     2941 acaccgacca gaacagctcg atgtaccaga ccgtgacgct cgccgcgggc gagtacaccc
     3001 tgagcttctg ggtgcaggac cgcgccggca ccgacgccgc gaccaacggc ctgagcgtgt
     3061 cggtcggcgg cctgctgccg acccaggtgc tggcgggcgg tgcctatccc gagtggacga
     3121 aggtcaccga gaccttcgac gtcgggaccg cgaccacgac cacgctgacc ttcgccgccg
     3181 ccggcgtgtc cgactcgctg ggcacctcgc tcgacaacgt gtcgctgacg gccgtgcccg
     3241 aaccctcggg cctggtgctg gcggcctcgg gcttggcgct gctgggcctc gcgcgccgtc
     3301 gcgcccgcaa ggcctgattg ccgcgcggcc ccggccgcga atcgcctgaa acgcaaaggg
     3361 acagcgccgc gaggcctgtc ccttcgtcgt ctgcggcgct gtcgggccgg tcaggtcagg
     3421 tcatctggcg gaagcgcagc gcctcgatcg cgcggccggt gccttcgtag agcacggcca
     3481 ggttgcgcgc cgcgagcgag ctgccgcgcc ccgcgaccga gccctcgagc tccggccgct
     3541 cgccgatctc caggcagcgc agccacgcct gctcgatcat cggcagcagc tcgccggcgc
     3601 gcgcgggttc ctcggtcgcc cagtccagca gcaggtcgcc gagcgcgaag aagaagtcgg
     3661 gcgagtcggc ccaggccgcc atcgacgcct cggcgagctg caccgcctcg ccatggcggc
     3721 ggcagcgctt gagggcatgg atctcgcgca cgaggcgatc gtggtgccag cccggcacgc
     3781 cgccgaccag ggtgacgccg ccggcgccgt gcgaggcctc gcagccgcgc gagcgggcga
     3841 agtgggacgc tgcctcgacg tagcgctcgt agacgtcgtg gtccttgccg agctggtagt
     3901 gcaggtaggc gtcgtcgggc gactgggcga gggcgcgttc cagcaggccc gcgttgcggc
     3961 cggacttggc ctgcagcgcc tgcgcgcggt agccgtcgtg ggcgagggtg gtcgcgagct
     4021 ggcgcaccgg cagcgcgtgg cgcggctgct cgtgcacgcg gccttcgtag cgcacgccgc
     4081 gcggcagcac gcgcggcagc cagctgggcg cgacgctgat gcccagcggg gtatcgaact
     4141 ggctgtcgac gcgcagggcg ccgacgaact ccggcgcggt gtggcgcagc gcgagcagct
     4201 cgagcccgtg ctcggcgatc cactcgtcgg cgtcgaccac cacgttccag tcggcgtcgg
     4261 cgagcgcgag cgccgcgttg cgcgcggcgg agaagtcgtc gacccatgcg aagtgcgcca
     4321 cgcgcgcgcc cgccgaacgc gcgagcgcca cggtggcgtc ggtcgagccc gtgtccacga
     4381 cgacgagctc gtcgacgcag ttgcgggcgc tcgcgagcgc ccgcacgatg cgcgcggcct
     4441 cgttgcgggc gatcatcacc agcgcgacgc gataggcggc catgttcact ccgcggccag
     4501 cgccgcgggc ggcaggccgg ccaggtggcg ctcgtgcatg cgcaggtaca gcgcgtcgag
     4561 atcgcgcgcg agccgttcgg catcgaacag cggcagcgcg cggcgatggg tctcgagatg
     4621 ctgcttgagg ccctgcagcg tctgcggttc gttggcgagc gcggtggcga aggcgacata
     4681 gtgcgcggcg tcggcgcagg cgagctcgcc caggccgcag gacgcgacga ggctggccgc
     4741 cacgcgcgac gcgaaggtcg gccccggcac cgtcagcacc ggcacgccgg cccacagcgc
     4801 ctcgctggcg gtcgtgtgcg cgttgcaggg ccaggtgtcg aggaacaggt cggccgcgcg
     4861 cagccgcgcg atgtggccgg ccaggcccag cttcggggcg aagatgacgc gttccgacgg
     4921 cacgccgcgg gcggcgagct cgcgcagcag gttctccttg gcgtgcgggt tccaggccag
     4981 catccacagc acggcgcgcg gcgcgccggc caggatcgcc gcccacaggt cgagcatgtg
     5041 cggcgacagc ttgtaggtct ggttgaagca gcacagcacc acggcgtcct cgggcaggcc
     5101 gagctccgcc cgcggcgggc agggcggcag cgcgcggctc gcgtcgttgg gctggtagca
     5161 gccggccagc tgggcgatgt gctcgctgta gttcgcggcg tgggacagcg gcgtcgtgat
     5221 gcggtcgccg atgaagtagt cgatgaagtc cgcgccgctg gatgccgggt agccgaggta
     5281 gctcgcctgc accggcgccg gtcggtaggc cagcaggtcc atccggctgt cgcgggtgtg
     5341 gcccttcagg tcgaccgcga tgtcgatgcc gtcgtcgcgc atgcgctggg cgatcgcctt
     5401 gtggccgagg tcgttcacgt cgacgaagtg ctcgcaggcg gcgcgcacgc ggcgcgtcac
     5461 gtcgtggccg tcgtccgggc tgtgcgaata caggaacacc tcgaagcgct cgcggtcgcg
     5521 gcgctcgagc agctcggcca tcagcaccgc ggtcgcgtgc tggtagaagt cggccgacag
     5581 gtagccgatg cggatgcgtc ccggcttgcg cggcccgggc ggcggcagcg gc
//
LOCUS       DASVLN010000385         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_234102, whole genome shotgun sequence.
ACCESSION   DASVLN010000385 DASVLN010000000
VERSION     DASVLN010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234102"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1315)
                     /locus_tag="VF453_09400"
     CDS             complement(185..1315)
                     /locus_tag="VF453_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7687911.1"
                     /translation="MIRGINGLDVHVLEGGHESAGRPLALLLHGFPDLAYGWRHLIPI
                     LADAGYHVVAPDQRGYGRTTGWVDGYDTPLEPFGVLNMARDAIALVSALGYRRTAMLV
                     GHDIGSPIAAYCALARPDVFPSVVLMSAPFPGVPAFPFDTADDEPSHGRPDTDVRTLA
                     AALAALDPPRQYYQQYLGSRRANEDMRHPPQGLHAFLRAFFHVKSGDWAGNRPHPLKA
                     RTAAELAQMPTYYVMDLGKTMPETVAPFHPSAGEVAACRWLTEPELGVYVAEYGRTGF
                     QGALQAYRIYADPALTSELRLFSGRTLDVPSLFIGGKSDWGPYSAPGALEAMQAKAAT
                     KMRDVVMVDAAGHWIQQEQPARLGALLLAFAKEVGGSDRPRG"
     gene            1562..2188
                     /locus_tag="VF453_09405"
     CDS             1562..2188
                     /locus_tag="VF453_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7687912.1"
                     /translation="MTGKTDARDRAIDTAQRLFRIQGYTATGLTQILVESGAPKGSFY
                     FHFPRGKTQLAEEAIDRYVASRMGVLRGLSERTTGDAAEFVRQVFDTFAREMVATDFQ
                     YGCLMQNLANELPALDAELTQRVARGFVDSTAIIAAHFRGCGFTPARASSTAAALTAA
                     LEGARTIARLERTPAIFKALSDISCQRLAPPEADAAPTPRPRRARKPA"
BASE COUNT          353 a          772 c          790 g          324 t
ORIGIN      
        1 gtccgcagag cgtgaaatgc gccctgcgac agggccaagc gcctgcgctg ccgcctgatc
       61 gaggccgaca gacatgcgac ctgacgatac gtgtggcatc aacagggctg cgcagcgctc
      121 cgtgtcacgt tttgcagacc atccctaggg aaggaggatg cccctcgcct tgcggcttca
      181 cgtcctagcc gcgcgggcga tccgagccgc cgacctcctt cgcgaaggcg agcagcagcg
      241 cgccgagccg agccggttgc tcctgctgga tccagtgccc ggcggcgtcg accatcacga
      301 cgtcgcgcat cttcgtcgcc gccttcgcct gcatcgcctc gagggcgccc ggtgccgaat
      361 acgggcccca gtcgctcttg ccgccgatga acagcgaggg gacgtcgagc gtcctgccgg
      421 agaacagtcg cagctcggag gtcagggccg gatcggcgta gatgcggtag gcctgcaggg
      481 cgccctggaa gccggtgcgg ccgtactccg ccacgtagac gccgagctcc ggctcggtga
      541 gccatcgaca ggccgcgacc tcgcccgcgg agggatggaa cggcgcgacg gtctcgggca
      601 tcgtcttgcc gagatccatg acgtagtagg tcggcatctg cgcgagctcc gcggcggtcc
      661 tcgccttcag cggatgcggc cggttgccgg cccagtcgcc gctcttgacg tggaagaagg
      721 cgcgcaggaa cgcgtgcagg ccttgcgggg gatgccgcat gtcctcgttc gcccgccgcg
      781 agcccaggta ttgctggtag tactggcgcg gcgggtcgag cgcggccagg gcggcggcca
      841 gtgtccggac gtcggtgtcg ggtcgtccgt gcgagggttc gtcgtcggcg gtgtcgaacg
      901 gaaatgccgg cacgccgggg aacggcgcgc tcatcaggac gaccgacgga aagacgtccg
      961 gtcgcgccag cgcgcaatag gccgcgatgg gcgagccgat gtcgtgcccg acgagcatcg
     1021 cggtgcgccg atagcccaac gccgacacga gcgcgatggc gtcgcgcgcc atgttcagga
     1081 caccgaacgg ctcgaggggc gtgtcgtagc cgtcgaccca gcccgtggtg cggccgtagc
     1141 cccgctggtc gggcgccacg acgtggtagc cggcgtcggc caggatcggg atcaggtgtc
     1201 gccagccgta ggccaggtcg ggaaagccgt gcagcagcag cgcgagcggc cggccggcgc
     1261 tttcgtggcc gccctccagg acgtggacat cgaggccatt gatcccgcgg atcacgcgcg
     1321 agcggacgcc cttgggcagg gtgccgtcgc cgaaggagga catcgtcatc gcgctttccc
     1381 ctgttgattg ggcatggacg ggtcgcgagg ccgcgatcag gccggcggcg ccggtcatca
     1441 ggacggtccg tcgggacagg aagggtgggt ggttcggcat cgcctgatca ctactatgtc
     1501 gatcaccata gtatggcgat caccatagtc cgcgtcaagc aaacggtggt acgttcagcg
     1561 catgacaggc aagaccgacg ctcgtgaccg cgcaattgac accgcccagc gactgtttcg
     1621 catccagggc tacacggcga cggggctgac ccagatcctg gtggaaagcg gcgcgccgaa
     1681 gggatcgttc tacttccact tcccacgcgg caagacccag ctggccgaag aagccatcga
     1741 ccgctacgtc gcgagccgga tgggcgtcct gcgcggcctc tccgagagga cgacgggcga
     1801 tgccgcggaa ttcgtccgcc aggtcttcga caccttcgcc cgcgagatgg tggccaccga
     1861 tttccagtac ggctgcctga tgcagaacct ggccaacgaa ctgcccgccc tcgacgcgga
     1921 gctgacccag cgggtggcgc gcggattcgt ggactccacc gcgatcatcg cggcgcattt
     1981 ccggggatgc ggcttcacgc cagcccgcgc atcgtccacg gcggccgccc tgaccgcggc
     2041 gctcgaaggc gcgcgcacga tcgcccggct cgaacgcacg ccggccatct tcaaggcgct
     2101 gtcggacatc agttgccagc gcctggcacc gcccgaggcc gacgcggcgc cgacgcccag
     2161 acctcgccgc gcccgcaagc cggcctgatg cggacgccgc cgccctccgg cggatcgtcg
     2221 agccacgccg tgccccgcc
//
LOCUS       DASVLN010000386        35874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_395464, whole genome shotgun sequence.
ACCESSION   DASVLN010000386 DASVLN010000000
VERSION     DASVLN010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 35874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35874
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395464"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF453_09410"
     CDS             complement(<1..219)
                     /locus_tag="VF453_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687913.1"
                     /translation="MRPSSPHESSPGRSAEGSHGSAPHGACPASPGPADELTLAILRE
                     LAQAADGLSLPRLGKRLGVGASVLMRRAA"
     gene            complement(216..2543)
                     /locus_tag="VF453_09415"
     CDS             complement(216..2543)
                     /locus_tag="VF453_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX7687914.1"
                     /translation="MSEKKIEFYKGPAGGWGALKSVSQALLRQDIPVKGAKTLLSANQ
                     PDGFDCPGCAWPDRNHASTFEFCENGVKAVASEATARRVTREFFAAHTVAELAARSDF
                     FLEDQGRLTEPMAYDAASDKYVPISWDDAFALVARHLDALPHPDRALFYTSGRASNEA
                     AFLYQLFVREYGTNNFPDCSNMCHEPSGSAMRPQIGVGKGTVTLEDFERADAIFVFGQ
                     NPGTNHPRMLGELRAASKRGARIVSFNPLRERGLERFADPQSKLEMATLGSTPISTHY
                     FQLRVGGDFAAVKGMMKHVVEREDAGGDVLDREFIAEHTTGIDALLDDLRAESWSLLE
                     QESGLSEEQMRAAADIYLGAERVIACWGMGITQHAHSVATIQMILNLLMLRGHLGRPG
                     AGACPVRGHSNVQGDRTMGIWEKPPADFLDRLREVFGFEPPRRPGADTVEAIAMMRDG
                     RADVFVALGGNFASAAPDTYETWKGLRRCALTVQVSTKLNRSHLVHGREALILPCLGR
                     TEIDVQAAGEQGVTVEDSMSMVHVSYGINPPASEQLLSEPAIVARLAAATLGTRSRTR
                     WNWLVEDYDRIRELIAAVFPDFAGFNDRIRVPGGFRLRNTAGERVWATDSGKASFRVH
                     PVTLDTPVHRARAQRRDAVVFTLFTTRSHDQYNTTIYGHDDRYRGVFGQRRVVFINAA
                     DLRHIGCRDGDWVDLHTVATDGVARRADRFRLVAYDIPRGCIAAYYPETNPLVPLASV
                     AVGANTPTSKSIPVTLTPHAGAGEVSGVDETFESA"
     gene            2907..3581
                     /locus_tag="VF453_09420"
     CDS             2907..3581
                     /locus_tag="VF453_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7687915.1"
                     /translation="MSNKTLAALAAIAATLAVAHPAQAADVTVGTYTSGNCYPFSCMA
                     GDSTLQYQQIYSAGAFGSGAIRIDDVSFFKSDAGQMTPSDYTVSFWLTASSTTGMSSS
                     AAANEGTLLSNFGSFHVGGTMPDTLTLSGSSFVYDPSQGNLLMDVEVTSGAAPWGYDS
                     FFEADYSNSNVTTRGYGSDGTLFATSQQGALVTRFSTSAVPEPGALALMLAGLGLVGV
                     TARRHA"
     gene            3916..5310
                     /locus_tag="VF453_09425"
     CDS             3916..5310
                     /locus_tag="VF453_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HEX7687916.1"
                     /translation="MAKSSRLTPVAIERTKPGAKDIWLSDDDGTRGGGRLVLRISPSG
                     TRLFYFRYSIDGARTQIPMRPWTKEPAEGRFTLEQARAVARSYSELHRDPATRDVAAY
                     LEQQRQAAAEAKQAEAEAKRAAAEAVQRHEKYTLLALCNWYQKHLAALGKPSAKSYKS
                     LIACHIEPTDIAKLPANAVTPKQVAAIIRSIVEAGKGRTGGHVRSILHAAYELASKAE
                     LDGTIPAELILFAIEANPVASTASMSKYNKTRDRSLSSAELGAFWRRLRDWPDPVPLS
                     VRALRLSLLLGGQRALQLLRVRRIEDVNLEDEIITLYDPKGRRAEARVHALPLTADAK
                     AEVEFLIVHSETLNSPWLFASQDSKLNNGTLSKQVNVISKEFIADETCTRPFRFDDLR
                     RTVETTMASLRISKDIRAQIQSHGISGVQARHYDRWEYMDEKRATLETWAAHLASVAD
                     NKPRASNVHQLKQA"
     gene            5712..6380
                     /locus_tag="VF453_09430"
     CDS             5712..6380
                     /locus_tag="VF453_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687917.1"
                     /translation="MAEAILAPSSGSSSPSPAGDPCPTYTAFCSDVSWKLSELHFRLK
                     AAIKLLTTSAEIDADVGDGLALSFVLCGLANSADALAEEVQNSELRFLAKQAGYEHHS
                     SALGDPMVANDEDLRDASDHKPNAAPAVSAGDAQARLAAAQQIFGRLANGILTATSVL
                     RDNSEDANAAHLAADALERLGWMADQGSVLAGEPRPPVNGEADEWMLGGLMRQALGDH
                     RLQG"
     gene            6458..6679
                     /locus_tag="VF453_09435"
     CDS             6458..6679
                     /locus_tag="VF453_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687918.1"
                     /translation="MAVHTEDAGLVPGSMTLAALVEAKTMSESIRTLVQNILDETPQE
                     GASMLEAIIACTLRLDSAVDPVIERAELT"
     gene            6676..7209
                     /locus_tag="VF453_09440"
     CDS             6676..7209
                     /locus_tag="VF453_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX7687919.1"
                     /translation="MKQMLHRPIYGGVFGHGVYSLATMPTISNGLHAVRWLVIEPRRG
                     TVLSVADEKLAAISSARRLLAGLSPPDAANDARWEQAALWADLPIDRPPRVRPVSRRR
                     REVFARSEGRCHYCGTALTLDGRWHVEHMVPRALDGEDSAPNLVAACVPCNLAKRDRT
                     ALEWVVSRDEGKHVHDR"
     gene            7256..7576
                     /locus_tag="VF453_09445"
     CDS             7256..7576
                     /locus_tag="VF453_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687920.1"
                     /translation="MRDVDALSNSFYDELGRNELADPVDYLAVALLVESSVQKNFCDS
                     GAQHREGFVRALAELIQNVMDGNDVPRPQDGWDPLASSAAAFSAPQLAALAISAARGS
                     SRQP"
     gene            8140..8349
                     /locus_tag="VF453_09450"
     CDS             8140..8349
                     /locus_tag="VF453_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEX7687921.1"
                     /translation="MTDSASHELPATGYVRQSALIPKVIPFSSATLWRKVKSGDFPKP
                     IKLSQRVTAWKVEDVRAWMQARNAA"
     gene            8359..8685
                     /locus_tag="VF453_09455"
     CDS             8359..8685
                     /locus_tag="VF453_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687922.1"
                     /translation="MNSLNGAAGAWIGPPKNDEAEATGGSLGLWDQSKTNAQDRSELA
                     AHDQAATEQHKAFSTLQAKFAIAGFGLHAMSDGSFLACRWNLSRPLPSLAAAGVFLRM
                     VGGARG"
     gene            8678..9607
                     /locus_tag="VF453_09460"
     CDS             8678..9607
                     /locus_tag="VF453_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX7687923.1"
                     /translation="MAEWHPTLAEVAHAVLGVELPALKPGTFVRFRGAADRAGQKSAY
                     AKLFPDEHGAIFGDWKLGAEGSWSARNQADMTPAERVAYRAAVRAAKEKREREQASEW
                     RRSGDRIRAILRGSRPIVDGDPAALYLRRRVGLDASALPQCLRIAYLPCIEDGIEIGV
                     FPTLIAPLVKGTETVALHRTYLTEQGRKAPVPTVRKLTPAAGILSGCAIPLSAPSGGA
                     IGVAEGIETALAASRLSGLPTVAAYSASNLASYRWPREARRVIVYGDNDPAGLAAAEG
                     LRSRAAAAGVSCQVVIPEKAGQDWCDVLQEGRE"
     gene            9604..10830
                     /locus_tag="VF453_09465"
     CDS             9604..10830
                     /locus_tag="VF453_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7687924.1"
                     /translation="MSEQDVLNAQAAARSGVILTNGSTLRPEPVNWLWEGWIACGKFH
                     ILGGAPGQGKTTLMLAMAAAITCGGRWPDGSRAPQGNVVVWSGEDDPRDTLLPRLLAM
                     SADPSRVYFLTGMREGDEERSFDPSTDMLQLTAEMEKIGDVKLLIIDPIVSAVQGDDF
                     KNNQVRRSLKPVVDLADATGCAVVGITHFGKSTAGRDPTERLVGSVGYAALARVVMAA
                     AKSKPLGDEAERRILVRTKSNIGPDDGGFEYRLRQTELDAFPGVTASCVEFGAAIDGT
                     ARDLLADAEALPDNDGDVHLNDAADFMRKIMTDVLGKSAGPMESREVDRLMKAEGYTT
                     KAIRRARESLGIVIKRTGFGEQTRTFWTLPDGPLMPTSATRAHVPEWARVGTNGARVA
                     DASRFDDTVTHDEEAF"
     gene            10830..11330
                     /locus_tag="VF453_09470"
     CDS             10830..11330
                     /locus_tag="VF453_09470"
                     /inference="COORDINATES: protein motif:HMM:NF036900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687925.1"
                     /translation="MSARKLLNSIHAAGMAIVLEGERLIVRPGSLLTSDLRESITAMK
                     PELVRLLQASNSPAFERPHALPPHELDRAHADAWGEEAIVRFVDRVGALRGAGLGEQD
                     AEDLAERLHLRDLDGADQSVCVVDCKHYSVGLCRNHREASISHELGAFATTPQRCPGI
                     AWKAIQ"
     gene            11518..11757
                     /locus_tag="VF453_09475"
     CDS             11518..11757
                     /locus_tag="VF453_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687926.1"
                     /translation="MNQLTIRRRIALTEGQRKAINQAAHVLAQQSFGRIRDEMLRKAR
                     AMLDEGHSSDEVSEAIRSWIVFDAWAERRAEGGAL"
     gene            11754..12011
                     /locus_tag="VF453_09480"
     CDS             11754..12011
                     /locus_tag="VF453_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687927.1"
                     /translation="MSIKAPAPTVQEDEPAPRRTRRSKPERTEQTTAVLVQASTLLAE
                     PASAAVPTMPQFMPWGESAEDFNSRMAGLADRLAMISGAKR"
     gene            12008..12220
                     /locus_tag="VF453_09485"
     CDS             12008..12220
                     /locus_tag="VF453_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687928.1"
                     /translation="MSRRIEIEASDEVLKVIDRLGQRWGEKIRTKIIERAVITAEDAL
                     AHAEQHPAWTPPDLRGNRYLWRSGPR"
     gene            complement(12259..12531)
                     /locus_tag="VF453_09490"
     CDS             complement(12259..12531)
                     /locus_tag="VF453_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687929.1"
                     /translation="MFRFLAIGIALGLTACASHTEVMALGGDKYMVGYQQRGGLNSWT
                     EVKFEAVKLANAHCAGMNKTAEVLNAEETGARGWTPMNVEVTFTCH"
     gene            12738..12962
                     /locus_tag="VF453_09495"
     CDS             12738..12962
                     /locus_tag="VF453_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7687930.1"
                     /translation="MDTHACDLQAIEPRHIEALLTADETARVLDIDPMTLAAWRHQRR
                     ALPFVKIGRMVRYRQSDVRAFIDANVREAA"
     gene            12962..13363
                     /locus_tag="VF453_09500"
     CDS             12962..13363
                     /locus_tag="VF453_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687931.1"
                     /translation="MDDFLALAIQNYAQAELAQAQRNFLANAQCVPADGAPPPMQQPV
                     REAADATPVLVQDALEALGTRYAQQHALGGLLRALRNVPGVGDSVAAALAEGQETQLI
                     ANACDATRDAYFCAAEALLSVLIAPEKARLQ"
     gene            complement(13391..13708)
                     /locus_tag="VF453_09505"
     CDS             complement(13391..13708)
                     /locus_tag="VF453_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687932.1"
                     /translation="MDDRLKKVEDAIRLLAEEMSRQGGQLQAIEGAVLGILKVAGVDD
                     QVRHEVEQEFERRAAFNLGSSTNQPQVDTFEELSNLLRLAMDERRASPGQGRDAVDPL
                     DGG"
     gene            13763..14344
                     /locus_tag="VF453_09510"
     CDS             13763..14344
                     /locus_tag="VF453_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX7687933.1"
                     /translation="MRNFLKIASNVQTTPLLLALARQPLLWNEHTLRTQYPGTPHSEV
                     DDIWLRFQTLPEPGREVEVVDQHESVWYPAINALPEVRPLVFNLMAAVQGERLGRVMV
                     TRLRAGGRIYPHTDDGAHAEYYERFHIVLASGQGCKFRAGDEIVHMAAGEVWWFQNAL
                     EHEVINDSKDERLHLIVDIRCPQSRQSPGNGGA"
     gene            complement(14413..14706)
                     /locus_tag="VF453_09515"
     CDS             complement(14413..14706)
                     /locus_tag="VF453_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687934.1"
                     /translation="MELERLAALVSRQEGQIMALEIAVTALIATHPYREAVLQFFEGM
                     SRARLDQHLATTLPEEMRDGIEQMRDQLLRTAGAPRFGLAVIPTNTQPQTPSR"
     gene            14794..15261
                     /locus_tag="VF453_09520"
     CDS             14794..15261
                     /locus_tag="VF453_09520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7687935.1"
                     /translation="MTEHRGPTAYTVERWSDIREEMLPLLYRHWREVALNHDEVPLHI
                     DEKRYADLDAKDALHIVTVRRDGGLIGYHVAFVSTHLHYASTLHGITDVFWIAPECRH
                     GVTALRMFQTAERELKKLGVRKLFTATKLHLDQGSLFEHMGYRPVERLYSKLI"
     gene            15272..16030
                     /locus_tag="VF453_09525"
     CDS             15272..16030
                     /locus_tag="VF453_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687936.1"
                     /translation="MVAAAGIASAVIGAGGAIVASNKQAGAANRATDLQGQIYQQNQA
                     NLAPYMAAGTPALSRLSDLLGTSGNTGASGYGSLTKPFTVQDYLNNQDPSYQWMQQQG
                     QQALMSSAGAQNGVLSGAAMKDLLNFSQGLAANQYQAAYNRWTTNQGNTFQRLSNLAG
                     LGENAAAGAGNMGVQSGANMANTITGAGNAAASGIVGASNAVSGGLNNMAGYYYLQHN
                     TGNPLMSQGFYGNDSTGYTNAAPWTVGPSPDGGS"
     gene            16030..17589
                     /locus_tag="VF453_09530"
     CDS             16030..17589
                     /locus_tag="VF453_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687937.1"
                     /translation="MPIDPNIALGIQTGSPFQTVASMLNVQNGLQQNRLLQAQTQAAN
                     IANQANAANLGERNALRNLITDPRVLDASGNVDPSKYAVVAPQVAPTLGGDVSKSITG
                     AQTAAVTNAETAFKLKGAQADRGFQEAAALFQDPRIQGLTDQASPGQVNAAASAILEA
                     QDRATAAGVPRETMAVLTAPFLNVLHNNPAMLPQALKNVVVAGAQRSQQPTVMGPQYS
                     PISTQGGTQLVQTNAFAPGGVGNAGNPYPPPNQIIPSPGGPVITNTSNGQARPLTPAP
                     STPPMALRIPSGETTQTEQALQDQRTATQSQLADLPARAEVYRNIIDLANKPGLSTGS
                     FAEGVRNLSSRTGYNFFSGNAVDYNALGKYLAQEAARTASAMGPGTNAGLANAQAASG
                     STSYDPTTIKRIAYLSEANAAALPEYDKGLSAAIQARLQQQPDTDPVFAKRQFDQQWA
                     ATYDPRIYELSNAMKRGDGEQVEEIFRGLGVPGTFSNGKFQPSGPMTTAATALFSKAQ
                     RLKAMTAGAAQ"
     gene            17586..20318
                     /locus_tag="VF453_09535"
     CDS             17586..20318
                     /locus_tag="VF453_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687938.1"
                     /translation="MSDTVDYDSVLGLFTPPDADKQISNASAILGSLKGQSPQVVSRT
                     AAALASTTTHPAVAAAASQLQSDANTTMTTQTTRGHLVQHPDTNAAVDRARSALSPAS
                     VAFTPQEQATSDAMERNPWATNGFARAYASASPQDRADMLRSRTMVMPSNVDYDGVMG
                     LFQGKPAPSNGMPAMPTTPQSLQVDQVKPGSDPYSDALYAKTGSGDYTRPGETYWQAY
                     GRNAAYDQAMRNAGLRKPASLLDLAGNAVSGVAGAIAGGYHGLLSLGQGTSTAGTNAE
                     NTAQALTYQPTNPESRAVLDIVNSNANPLNWPGAVGHWIGDKLAEHGMPAVGAAAATL
                     GAAAPLVLGASSKARNAVASAGRSAGTTVADLVRPAAPAAPRVEPTLEPTGVQQSSGS
                     PASTAAAPAGARTQAQPNQAAPSPGQAAGPPGYLDPTLRVPSAPAAPRAPALSDGANG
                     VFAEAPTPAQASLPIEEQQRRAQVLKSIGLDNARESATSGDAKQAATDFQVSRTDTPG
                     GNAMSQVFARERQALNDYADNIVKDTGGTTMNDQSAKYSRGETIMAPLEALKDYYDSN
                     IARLYGEADERAQGVPTQLDQFRTTLGDDSLMTNSDRVQLRSAITAYAKKLGIIGDNG
                     SIFANGQQAETMRKYLNEQWTPQASGLIGKLKDALDDDVMQSAGEDVYGQARELRSQR
                     ARVLDDPNGIAKLMDASGPNGINRKVPVEKVADTITSLPADQLGHIVTTLKTVPDALQ
                     PQAQAALAEIKAQFAHNVAETGGKTAGQWNNKGVQRYLSGNSARLGIVFSPDEMQRFG
                     TLNDAGNILAHSQAYPGAAAQAHNLVNAGTGARAVGLGLQSAGGAIGGTLGSVFGPVG
                     TAGGTTLGAALGGKGAGMMNDALSMHAFNKRMVKLADLLPTSKQSGLSDVGRTR"
     gene            complement(20540..20770)
                     /locus_tag="VF453_09540"
     CDS             complement(20540..20770)
                     /locus_tag="VF453_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687939.1"
                     /translation="MAFYKYGNFLAQHQGGEFDVVWNPGTVPAVSGIYRCPGCGKEIT
                     HIGGTRLPPQNHHQHTYTQGSVRWQLLVAHQG"
     gene            21099..21467
                     /locus_tag="VF453_09545"
     CDS             21099..21467
                     /locus_tag="VF453_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687940.1"
                     /translation="MTRFRQISALSAASLLSILPGASRADFEDDISDYVGYTIIAVKT
                     IDSFKDNSHERKDGFEGCEYDRVIVFTDGTAVTCRSYGYKYAYRPKAVILGQQMAFQG
                     RAVTILKMVVQGTAYDVASR"
     gene            21686..22222
                     /locus_tag="VF453_09550"
     CDS             21686..22222
                     /locus_tag="VF453_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687941.1"
                     /translation="MSKPLSIILATAALCLAPPATAWADDRAACAEADGSLLEGVVIA
                     RPQYALGKPLRGIPLSHTHIKLRGDQDGKTYDVAVDNVFAAGYDPNVEAVPAPLSTIT
                     PGQHLEACGIPYAGGMHWVHTNCGATPTPQDPNGWLNVLDAAGNAGPNLEDSQKYCYL
                     WPARAHMRHRRHAQHSVE"
     gene            complement(22400..22756)
                     /locus_tag="VF453_09555"
     CDS             complement(22400..22756)
                     /locus_tag="VF453_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687942.1"
                     /translation="MRFAVFSGDTLVGHSALEFGDPPMGVAFGKFIPTEGYRLIQDEC
                     RMIHTDQAALALTARTSAGDKLPCSGVSVLDYAAHADVGDEPYVEATVLGIPDPLYGE
                     LFPAHVAAYEKQFRGG"
     gene            complement(22810..23271)
                     /locus_tag="VF453_09560"
     CDS             complement(22810..23271)
                     /locus_tag="VF453_09560"
                     /inference="COORDINATES: protein motif:HMM:NF025451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4265 domain-containing protein"
                     /protein_id="HEX7687943.1"
                     /translation="MEKVRFKLDSEDWHGHSLERMWAKEVLGSDRLTLFQVQNSPFFA
                     KGVSFLDVVSVLRDEDGEYVFDEVVEKSGHSTLILLVPIESKAAFYADCWPEAETLGC
                     TYESGEIELSFGLAEMLSVDVLPTADRHTVRDWLVAGERASVFHYQESDLR"
     gene            complement(23553..24038)
                     /locus_tag="VF453_09565"
     CDS             complement(23553..24038)
                     /locus_tag="VF453_09565"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEX7687944.1"
                     /translation="MRQLILSIALSIVSICVHAEGQGDDDSGGLIDQKIPDPSERVIQ
                     EMLRASHSLPEQLLRESFRQCDRDNLTLRICASYRLTEQDLRLNHVYSRLLQTTKNEH
                     TDALLIKAERAWLGFRDAQCEFEGAESQLGGTMPIAVVADCKADLTERRAESLEALTR
                     P"
     gene            complement(24329..25192)
                     /locus_tag="VF453_09570"
     CDS             complement(24329..25192)
                     /locus_tag="VF453_09570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7687945.1"
                     /translation="MILASREAPPSMRLQAALASVFIGAWMAAGWLLALPPNAYLVLG
                     VPLVLAYQLVILHRPVRALWRFDGAPLGFDRSIVTVGLMLATWPALSLVASFVTSARV
                     GVDVAAWCLCAAVGAFGLAWWLVNAWRQPQFWRGGQQPAFFALVVVTGVMLFALSAWM
                     SGARPHLPPATGLATFISDAWLYACVCFVLEEVVFRGAIDPAIVPDAPVTGLRAHATA
                     VFVSLLWGAWHFPLLPFGHGSIHSMASTLAFHVGVGWPMTLLARRARNLGPTCLAHAA
                     IDAFRDVITSV"
     gene            complement(25201..25527)
                     /locus_tag="VF453_09575"
     CDS             complement(25201..25527)
                     /locus_tag="VF453_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7687946.1"
                     /translation="MSTFGARLKQARQLAGLSQRQLGILAGIAPGSSSARMNQYERET
                     HEPPATLVRKLARVLGVPTPFFYAEKNEHAELLLAFAKLPAAQRRKILTEVRKAAVNT
                     AAKQEA"
     gene            complement(25584..26009)
                     /locus_tag="VF453_09580"
     CDS             complement(25584..26009)
                     /locus_tag="VF453_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX7687947.1"
                     /translation="MVKVIDSAQRELRVLAYSFTSVPVTEALVRARHRGVDVRVIADQ
                     KENISQDRSGKARAALGALQAAGADVRTISVYSIHHDKVVIADGQTVELGSFNYSAAA
                     AHANSENVLVNWSNPKLASVYLEHFARNYAQAQPFVPSY"
     gene            26254..27225
                     /locus_tag="VF453_09585"
     CDS             26254..27225
                     /locus_tag="VF453_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX7687948.1"
                     /translation="MCYSAQVYADWKKYTRMFGATMSVGEFYRTFWRRNNEPEAKVKI
                     PKAMEHAFADPQTDDERRIKAEIDAWHASEATRLEQELFAQRKRLADAERVLAGPKPT
                     KKAADDARIAPDKIDRARRALADLQRTELEPRDSRIFPGVYAPVMVVQDGELVVMPMR
                     YQCRPCGSPAFFDTKFPGTYNARRDNLGGKFWQSLFGFSHGIMLASSFFEHVRRHKAE
                     GRELGDGEAEEDMILEFKPAGVEEMLVACLWARWTSPGQPDLLSFAAITDTPPPEVAA
                     AGHDRCIIPIKRGNVDAWLNPDASQLDALHAILDDRERPFYEHRLAA"
     gene            complement(27201..27689)
                     /locus_tag="VF453_09590"
     CDS             complement(27201..27689)
                     /locus_tag="VF453_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687949.1"
                     /translation="MFSGTSHWTPQPNDFSRACWRCEHWCGDSGGIGGCARDIVKDGH
                     IRAASARPETGCAFWVRCPGLDLPVDWTPAGFQRSANHVPIARLEAMRAASREEWRHQ
                     DEQRALEAARKAARAAAKQPLDDHFAPAELSLDTCWRIADDLMARQGAFLTRRYAASR
                     CS"
     gene            complement(27756..28361)
                     /locus_tag="VF453_09595"
     CDS             complement(27756..28361)
                     /locus_tag="VF453_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687950.1"
                     /translation="MAQLPTGCSAMQPSASVTVAVCQPAPESIASKFVSSLPSVALSV
                     VALLLSWKTFAYNQEKDRRARKQSIQDDFWLRKVVSPVSIEPFLEHINELCARLPSVG
                     ATTEEANAFWSKEVLELGRFEVAFQTLALIDRQLLEDVLKKFEYLHDRLADYNGELRA
                     YIDTPTAPTPDREGTTRYLLSCSMELLGLIKAHQTRVWSDG"
     gene            complement(28487..28810)
                     /locus_tag="VF453_09600"
     CDS             complement(28487..28810)
                     /locus_tag="VF453_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687951.1"
                     /translation="MNLEDRTIDFAKIEGPVFTGRNRGERLREEMQLDRHDAAGDVMD
                     VRIPENIYAISSSFFLGMFGPSVVKAGSKQRFYEQYHFQSPRFIREALDGYVSRALQA
                     RNLFT"
     gene            complement(28807..30393)
                     /locus_tag="VF453_09605"
     CDS             complement(28807..30393)
                     /locus_tag="VF453_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687952.1"
                     /translation="MPEAIEDLAWLPLMAAAWWVKIRLEDWLNGGRSKSPLTGRCENA
                     AHGLHVEQPKIHSLTNSEQAPEAGGGEVAIHLAKIDVPRYLKEKRGRKLERRQHRLWK
                     AKRERLESEDELRAHFGKTSKIVEMLAVEHAPAKKQFGVRRRPEAKRGGRLRFVLPST
                     LSIIDDPVRTLGAINQLAHEMRSKRLGSLFLDFGKVTQYDLGANALLDVLVDELATQA
                     RQTGRSIRWHGRYPADPAHRRFIRAMGVIRRLRIAHEYPTKEEEGRLRVFDARCKHYV
                     RAARPREATRNAQVTAAFADHIDECLATIGRRLVDIAKAELCLYVGEIIDNAEEHSGM
                     YDWAIQGYLDTQLDVPMCEVVIFNFGRTIAASFEDLPERSYTRQHVQTYLDLHEKTGL
                     FRKGGTREGLFTLIALQGCVSTKNTRPEDTRGNGTVDLISFFERVHEECKGGQSPGAR
                     MVILSGSTYALFDGTYRMEANAAGNRIIAFNTFNDLRMKPDDRFLRHLHGVKFPGTLI
                     GLKFPLSTTASTEATPGEAV"
     gene            complement(30397..30531)
                     /locus_tag="VF453_09610"
     CDS             complement(30397..30531)
                     /locus_tag="VF453_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687953.1"
                     /translation="MSEERQRARAGLIAACVAAAVLAFAVAGLAYVASYFSRFHTGWR
                     "
     gene            complement(31861..32100)
                     /locus_tag="VF453_09615"
     CDS             complement(31861..32100)
                     /locus_tag="VF453_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEX7687954.1"
                     /translation="MSSLHLHFMTDPAVPSAFFREAQLVPNVIPVSPATLWRWVREGN
                     FPKPVRLSSRITAWRVEDVERWIASRTPESIDEAE"
     gene            complement(32171..32941)
                     /locus_tag="VF453_09620"
     CDS             complement(32171..32941)
                     /locus_tag="VF453_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687955.1"
                     /translation="MPVDPNLRRFTGIDALMAARTGHWRSGLYAADEVTWAKWAGQEA
                     LELWQLVALHSALDPDSLGIGLHGVRSTFLTVAFFDSVPVHPPPVIDPDDPLRRLAKN
                     LRSASEAFAGGQLKEHVDPHSTRNGTSNDRWANDDYWDRRVAVSDFHAWSLRVNLPVV
                     DGWPARSVAASGPRWPWGSHETQLLRELAEIGEHWRLTSEGGPHDPDDEGTVPKAEVI
                     APLLEAKGISEKVRQAMFTILRPDGIRPGPRRRRPLAK"
     gene            complement(33084..33160)
                     /locus_tag="VF453_09625"
     tRNA            complement(33084..33160)
                     /locus_tag="VF453_09625"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33124..33126),aa:Ile,seq:cat)
     gene            complement(33186..34232)
                     /gene="hrcA"
                     /locus_tag="VF453_09630"
     CDS             complement(33186..34232)
                     /gene="hrcA"
                     /locus_tag="VF453_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX7687956.1"
                     /translation="MLDERAKTLLKTLVERYIADGQPVGSRTLSRASGLELSPATIRN
                     VMADLEELGLIASPHTSAGRVPTARGYRLFVDTMLTSRALEADDASQLLSELTELGAG
                     REALQPDQPQRVIAQAASMLSNLSSFVGVITAPRRASIFRHIEFLRLGERRVLVILVA
                     PDGDVQNRVIITAQDHDQAQLVAATNFVNAHYGGLSIEDIRERLKTELQSLSDDIAGL
                     MRAAVQAGSEAIAESEEQVVVVGERNLLGVQDLHHDMRSLRDMFDVFEQKTQLMRLLE
                     GSSRAEGVRIFIGGESRVVPFEALSVVSAPYEVDGRVVGTLGVIGPTRMPYDRMIRIV
                     DITSKLVGNALSQR"
     gene            34372..35244
                     /locus_tag="VF453_09635"
     CDS             34372..35244
                     /locus_tag="VF453_09635"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX7687957.1"
                     /translation="MSTPFRHVALFGKYQAEGVRPILEEIAQFLVRAGVEVSVDRATA
                     LNTGWNDFGAMEIDELAQVCDLAVVVGGDGTMLGIGRELARHGIPLIGINQGRLGFIT
                     DIPHADWRDALVPMIRGDYEAESRPMIEGGVWRDGQQIFQGFALNDVVVRSANPSMIE
                     VRVHVGSEYVANIRSDGVIVASPTGSTAYALSAGGPILHPGLGGWAMVPVASHTLSNR
                     PIVLPDDREVTLELVQGKEASANFDMHGLASMLLGDTVRMQRSAHKARFLHPLGWSYY
                     ATLRNKLHWHEGTT"
     gene            35392..>35874
                     /locus_tag="VF453_09640"
     CDS             35392..>35874
                     /locus_tag="VF453_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7687958.1"
                     /translation="MLRRLSLKDFVIVDALELEFDAGFSVLTGETGAGKSILIDALQL
                     ALGARAEGGVVREGCPRSEITAEFDCPPALQPWLDEAGFALDETLLLRRVVDAQGKSR
                     AFVNGSAATVAQLREVAEHLVDIHGQHAWQSLTRPAAVRALLDEQAGLDAAPVAAAYA
                     A"
BASE COUNT         6544 a        11787 c        11554 g         5989 t
ORIGIN      
        1 cgcggcgcgg cgcatcagca cgctcgcgcc cacgcccagg cgcttgccca gccgcggcag
       61 ggacaacccg tccgccgcct gcgcgagctc gcgcaggatc gcgagcgtga gctcgtcggc
      121 cgggccgggg ctcgcagggc aagctccgtg cggcgcactg ccgtgcgagc cctctgcgga
      181 gcggccgggc gaggactcat gcggactcga cggtctcatg cggactcgaa ggtttcgtcc
      241 acgcccgaga cctcgcccgc gcccgcgtgc ggcgtgagcg tcaccgggat cgacttcgac
      301 gtcggcgtgt tcgcgccgac cgcgaccgag gcgagcggca cgagcgggtt ggtctccggg
      361 tagtaggccg cgatgcagcc gcgcgggatg tcgtaggcga cgaggcggaa gcggtcggcg
      421 cggcgggcga cgccgtcggt cgccacggtg tgcaggtcga cccagtcgcc gtccctgcag
      481 ccgatgtgcc ggaggtcggc cgcattgatg aacacgacgc gccgctggcc gaacacgccg
      541 cggtagcgat cgtcgtggcc gtagatggtc gtgttgtact ggtcgtgcga gcgcgtcgtg
      601 aacagcgtga agacgacggc gtcgcggcgc tgcgcgcggg cgcggtgcac cggcgtgtcg
      661 agcgtcaccg ggtgcacgcg gaagctcgcc ttgccggagt ccgtggccca cacgcgctcg
      721 ccggccgtgt tgcgcaggcg gaagccgccc ggcacgcgga tgcggtcgtt gaagccggcg
      781 aagtcgggaa acacggccgc gatcagctcg cggatgcggt cgtagtcctc gaccagccag
      841 ttccagcgcg tgcggctgcg cgtgcccagc gtcgccgcgg ccaggcgcgc gacgatcgcg
      901 ggctccgaca gcagttgctc cgacgccggc gggttgatgc cgtacgagac gtgcaccatg
      961 ctcatcgagt cctcgaccgt caccccctgc tcgccggcgg cctgcacgtc gatctcggtg
     1021 cggcccaggc agggcaggat caacgcctcg cggccgtgca cgaggtggct gcggttcagc
     1081 ttggtcgaga cctgcaccgt cagcgcgcag cggcgcaggc ccttccaggt ctcgtaggtg
     1141 tcgggagccg ccgaggcgaa gttgccgccc agcgcgacga agacgtcggc gcggccgtcg
     1201 cgcatcatcg cgatcgcctc caccgtgtcg gcgccgggcc tgcgcggcgg ctcgaagccg
     1261 aagacctcgc gcaggcggtc gaggaagtcc gccggcggct tctcccagat gcccatcgtg
     1321 cggtcgccct gcacgttgct gtggccgcgc accggacacg cgcccgcccc ggggcggccg
     1381 agatggccgc gcagcatcag caggttgagg atcatctgga tcgtcgccac cgagtgcgca
     1441 tgctgcgtga tgcccatgcc ccagcacgcg atgacgcgct cggcgccgag gtagatgtcg
     1501 gccgccgcgc gcatctgctc ctcgctcagg ccggactcct gctcgagcag cgaccacgac
     1561 tcggcgcgca ggtcgtccag cagggcgtcg atgccggtcg tgtgctcggc gatgaattcg
     1621 cggtcgagca cgtcgccgcc ggcgtcctcg cgctcgacga cgtgcttcat catgcccttg
     1681 acggccgcga agtcgccgcc cacccgcagc tggaagtagt gggtgctgat cggcgtcgag
     1741 ccgagcgtcg ccatctcgag cttgctttgc gggtcggcaa agcgctcgag tccgcgctcg
     1801 cgcagcgggt tgaagctcac gatgcgcgcg ccgcgcttcg aggcggcgcg cagctcgccc
     1861 agcatgcgcg gatggttggt gcccgggttc tggccgaaga cgaagatcgc gtcggcacgc
     1921 tcgaagtcct cgagcgtgac cgtgcccttg cccacgccga tctgcggccg catcgccgag
     1981 ccgctgggct cgtggcacat gttggagcag tcggggaagt tgttcgtgcc gtactcgcgc
     2041 acgaacagct ggtagaggaa cgcggcctcg ttgctcgcgc ggccggacgt gtagaacagc
     2101 gcgcgatccg gatgcggcag cgcgtcgagg tggcgcgcga cgagtgcgaa cgcgtcgtcc
     2161 cacgagatcg gcacgtactt gtcgctcgcc gcgtcgtagg ccatcggctc ggtcaggcga
     2221 ccctggtcct cgaggaagaa gtcgctgcgc gccgccagct ccgccaccgt gtgcgcggcg
     2281 aagaattcgc gcgtgacgcg gcgcgccgtc gcctcggacg cgacggcctt gacgccgttc
     2341 tcgcagaact cgaaggtcga cgcatggttg cggtcgggcc aggcgcagcc ggggcagtcg
     2401 aagccgtcgg gctggttggc cgacagcagc gtcttcgcgc ccttgacggg gatgtcctgg
     2461 cgcagcagcg cctggctcac gctcttcaac gcgccccagc cgccggccgg gcccttgtag
     2521 aactcgatct tcttctcgct catccctgct cgttcgcggc gccggcggcg cggacggggc
     2581 gtcggccgcg gcgcggcgcc gcgccggtca taccctgtcc cgtatatgga aagtcgccaa
     2641 ccaggccggg aggcgacgcg ttggccggga agttatcacg gatggtgcgc cgcacaaata
     2701 tgcgctgcgc cgcctatggg atgggaagag tcgtcggtcg gcggcgtgca cttcgtcacg
     2761 caccgccgga ccgaacgcct tcccgagcac cgaatctcac cagattcggg gagccgccca
     2821 cgcgagggcg tgcgtgcagg ccgctatcgt gcacgtccgc gcagctcgac cgtgcatgcg
     2881 cccatccaag gctaagagga gacctcatgt ccaacaagac gctggcagcg ctcgccgcca
     2941 tcgccgcgac gctcgccgtc gcccatcctg cccaggccgc cgacgtcacc gtcggcacgt
     3001 acacgagcgg caactgctac ccgttcagct gcatggccgg cgacagcacg ctgcagtacc
     3061 agcagatcta ttcggccgga gccttcggca gcggcgcgat ccggatcgac gacgtcagct
     3121 tcttcaagtc ggacgccggc cagatgacgc cgagcgacta caccgtcagc ttctggctca
     3181 cggccagctc gacgaccggc atgtcctcgt cggccgctgc caatgaaggc acgctgctgt
     3241 cgaacttcgg ttcgttccac gtcggcggca cgatgcccga cacgctgacg ctgagcggct
     3301 cgagcttcgt gtacgacccg tcgcagggca acctgctgat ggacgtggag gtgacttcgg
     3361 gcgcggcgcc gtggggctac gactcgttct tcgaggccga ctacagcaac agcaacgtca
     3421 ccactcgcgg ctacggctcc gacggcacgc tgttcgcgac cagccagcag ggcgcgctcg
     3481 tgacccgctt ctcgacctcg gccgtccccg agccgggcgc cctcgcgctg atgctggccg
     3541 gtctcggcct cgtcggcgtc accgcccgac gccacgcctg acggcagccg ccccgccgga
     3601 cacgcccggc ccatccaggc cgcgccgcgc agggcctgga tccctgtcct gcagggaggg
     3661 cggcgaatcc gagcgcctcg atcgcgccgg agacgtcttg gaggtggtag gccgtgttgg
     3721 acttgaacca acgaccaaag gattatgagg cggatggcac cggatctcag gaaagctcac
     3781 accgtgtcga caggggcatc tcggtagcgc agaacctcct ttcaaaacaa tggcttacag
     3841 cgcttgacac ttcgcgcggt ctcagggaag ctcacacgtt aacggcaacg taacggcaac
     3901 gccccgagga ttcgattggc gaagagctcg cgactcaccc ctgtagcgat cgagagaacc
     3961 aaaccaggcg ccaaggacat ttggctgagc gacgacgacg gcacccgtgg cggcggccgg
     4021 ctcgtgcttc gaatcagccc cagcggtaca cggttgttct acttccgata ctcgatcgat
     4081 ggcgctcgga cccagattcc gatgcggcca tggacaaagg agccagccga gggtcgattc
     4141 acactcgagc aggcccgagc agtcgccagg tcgtactcag agctccatcg cgaccctgcc
     4201 accagagacg tcgctgcgta tctggagcag caacgccagg cggctgctga agccaagcag
     4261 gcggaagcag aagccaagcg tgctgccgcc gaagctgttc agcgtcacga gaagtacacg
     4321 ctcctggctc tgtgcaactg gtatcagaag cacctggcag cgctcgggaa gccttcggcg
     4381 aaatcgtaca agtcgctgat cgcgtgccac atcgagccca cggacatcgc caagctccct
     4441 gcgaatgcgg taacgcccaa gcaggtcgcc gcgatcatcc gaagcatcgt cgaggcaggc
     4501 aagggccgaa caggtgggca tgtccgctcc atccttcacg cggcctacga actggcgagc
     4561 aaggccgagc tcgatggcac gatcccggcg gagctgatcc tcttcgccat cgaggccaac
     4621 ccggttgctt cgacggcgtc catgtccaag tacaacaaga cgcgagatcg ttcgctgtcg
     4681 tctgcggagc tgggggcctt ctggcgacgg ctgcgggact ggcccgaccc tgtacctctt
     4741 tccgttcgtg cgctacgcct ctcgttgctc cttggcggcc agcgtgccct gcagcttcta
     4801 cgagtgcgtc ggatcgagga cgtcaatctc gaagacgaga tcattacgct gtacgacccg
     4861 aagggccgac gggccgaggc gcgcgtccac gcccttcccc tgaccgcgga cgcgaaagcc
     4921 gaggttgaat tcctcatcgt gcacagcgag acattgaact ccccgtggct cttcgccagc
     4981 caggattcca agctcaacaa cgggacgcta tccaagcagg tgaacgtgat cagcaaggag
     5041 ttcatcgccg atgaaacgtg cacgcgcccg tttcggttcg acgatctccg gcggacggtg
     5101 gagacaacga tggcttccct gcgcatcagc aaggacatcc gcgcccagat ccagtcgcat
     5161 ggcatcagcg gcgtgcaggc acgccattac gaccgatggg agtacatgga cgaaaaacgt
     5221 gcgacgcttg agacttgggc ggcacacctc gcatccgtgg ctgacaacaa gccgcgcgcg
     5281 agcaacgtcc atcagctcaa gcaggcctag ttatgtagat cttgcctact ggctattgat
     5341 ctacattttg tagtctgcta tcgtccacca tgagccgcca cgagagcgca tgacaccaga
     5401 tgagttcgat tcggcgctcg ccgagctagg ttggagcggc gccgatttct tgcgtcgagt
     5461 cgacgtggtg ccaaacacag tttggcgatg gaggaaggga acgacgccga tcccatcgtg
     5521 ggtcgatgaa tacctgaagg cactgatcgg aatcaaggcg ctctacgagg cacatgtcgc
     5581 agttgcgaag cgagagcctg gagacctaga ggccctgtaa ccgcgccggc cggcagccgg
     5641 caaagcgggc ccgcaaggtg gtggaacacc gagcgggccc tgaccacaac cacctgtatc
     5701 agaggtgact catggctgaa gccattctcg caccgagctc cggctcgtcc tctccttctc
     5761 ctgcaggcga cccctgcccg acctacacgg cgttctgcag cgacgtttcc tggaagctgt
     5821 cggagctgca cttcaggctg aaggcagcga tcaagcttct gactacttcg gcggaaatcg
     5881 acgccgatgt cggggacggc ctcgccctga gctttgtcct gtgcggccta gccaacagcg
     5941 ccgacgccct cgccgaagaa gtgcagaaca gcgaacttcg cttcctcgcc aagcaggccg
     6001 ggtatgagca ccacagttcc gctctgggcg atcccatggt cgccaacgat gaggatctcc
     6061 gggatgcgtc cgaccacaaa cccaacgccg ccccggccgt gagcgctggc gacgcacagg
     6121 cccggctcgc cgcggcacag cagatcttcg gacggcttgc gaacggcatc ctgaccgcga
     6181 cgagcgtgct tcgagacaac tccgaggatg caaacgccgc gcacctcgcg gccgacgcac
     6241 tcgagcggct cggatggatg gccgaccaag gctccgtcct cgctggcgag ccgcggccgc
     6301 cggtcaatgg cgaggctgac gaatggatgc tcggcggcct gatgcgacag gcgttgggcg
     6361 accaccggct gcagggctga agagccgccc cgacgaccca ggaggcgctg gctaccagcg
     6421 gccacttgat caacgcattc gataggaagg gcgccccatg gctgttcaca ctgaagacgc
     6481 cggtcttgtg ccgggctcga tgacactcgc ggccttggtc gaggcaaaaa ccatgtcgga
     6541 gagcatccga acgctggttc agaacattct cgacgagacg cctcaagaag gtgcgtcgat
     6601 gcttgaagcg atcatcgcgt gcactctccg gctggactcg gccgtagatc ccgtgatcga
     6661 gcgagcggag ctcacatgaa gcagatgctc caccgcccca tctatggcgg cgtcttcggc
     6721 cacggcgtct actcgcttgc gaccatgcca acgatctcca acggcttgca tgcggtcagg
     6781 tggctggtga tcgagccgcg gcgtggcacc gtgctgagcg tggcggacga gaagctggcg
     6841 gcgatctctt ccgcgcgccg cttgctggcc ggactttctc cgccggatgc agccaacgat
     6901 gcgcgatggg aacaggcggc cctatgggca gatctgccaa tcgatcgacc gccgcgcgtg
     6961 cgcccggtct cccgtcgccg acgagaggtc ttcgcgcgat cggaaggccg ctgccactac
     7021 tgcgggacgg cgctcactct ggatggtcgt tggcatgtgg aacacatggt tccacgagcg
     7081 ctcgacggag aggactctgc tccgaacttg gtcgccgcct gcgtcccgtg caacctcgca
     7141 aagcgcgacc ggactgcact cgagtgggtc gtcagccgtg acgagggaaa acatgtgcac
     7201 gaccggtgat gaccttcccg aggttcgggc aggtgctgac gcgctcctcg cgctcatgcg
     7261 cgacgtcgac gcgctgtcga acagtttcta cgacgagcta ggcaggaacg agctggccga
     7321 tcctgtcgac tacctggcgg tcgccctcct cgtcgagtcg agcgtccaaa agaatttctg
     7381 cgacagcggc gcacagcatc gcgagggctt cgtccgggcg ctcgcagagc tgattcaaaa
     7441 cgtcatggac ggcaacgatg tacccaggcc ccaggacggc tgggatccgc tggcatcctc
     7501 cgcggcggcg ttttctgcgc cccaacttgc tgcgttggcg atttctgcgg ctaggggttc
     7561 cagccggcaa ccatgaagcc gcacgagcag gccgagttcg acttcggcat ggcgcggcag
     7621 cagcgaatcg gccgagccct tcgagcagct cccggaggag tcagccgcgc tgtaccgctc
     7681 ggggacatgg acaagcttgt gcttgcttgc atggattcgc ttgcatttca gatcgctgac
     7741 gaagtgatgc gactgccaaa ggactctgca tcccggtata ccgcctcacg aattgctaag
     7801 ttcattgctt cgctgggcga cgtatctccg gagttcgcaa acgagacgtc tcgctacttg
     7861 aaagagtcaa ttgctcggca acgttaggct gttgaatttc gcttcccact tgcggtagcg
     7921 tgagacgcca tgcataatca gagcgccgca tgggactcgc cgaccgtgca gatgtgaaag
     7981 gcgcgctggg ttggcagcca gcgaagcgga tgtaggacgc tgagacgcga tccttgacac
     8041 tcggaaatag accgagagcc ctgcccgggg tcgtcatctt ccagacgtac ggattcatcg
     8101 cgccgcgagg cgcgtgtctc tcgtttcctg gagatccgtg tgaccgactc tgcatcccat
     8161 gaactgcccg cgacgggcta tgttcgccaa tcggccctca ttccgaaggt cattcccttc
     8221 agcagcgcca cgctttggcg caaagtgaag tccggcgact ttcctaagcc aatcaagcta
     8281 tcccagcgcg tgaccgcctg gaaggtcgaa gacgtccgcg cctggatgca ggctcgcaac
     8341 gctgcttgag gaggcgcaat gaactccctc aacggcgctg cgggcgcgtg gattggtccg
     8401 cccaaaaatg acgaagccga ggcgactggc gggtcgctcg gcctctggga tcaatcgaaa
     8461 accaacgctc aagatcgtag cgaactcgcg gcccacgatc aagctgccac tgagcagcac
     8521 aaggctttct cgacgctgca agcgaagttc gctatcgcag gcttcggtct gcatgcaatg
     8581 tcggatggca gctttctggc ttgccgctgg aacctgagtc gcccgcttcc atccttggcc
     8641 gctgcgggcg tgtttctgcg catggtcgga ggtgctcgtg gctgaatggc acccgaccct
     8701 tgcggaggtc gctcacgctg ttctcggcgt tgagctcccc gccctgaagc ccggcacgtt
     8761 cgtccgcttc cgcggtgccg ccgaccgagc cggccagaaa tcggcctacg ccaaactctt
     8821 tcctgacgag cacggcgcga tcttcggcga ctggaagctg ggcgctgagg gatcgtggag
     8881 cgcgcgcaat caggccgaca tgacaccggc ggaacgcgtg gcgtaccggg ccgcggtgag
     8941 agccgcaaag gagaagcgcg agcgagaaca ggcatcggag tggcgccgta gcggcgatcg
     9001 aatccgggcc attcttcgcg gttcacggcc catcgtcgac ggtgatccag cagcactgta
     9061 ccttcgtcgc cgcgtcgggc tcgacgcctc tgcactaccc caatgcctgc ggatcgcgta
     9121 cctcccctgc atcgaggacg ggatcgagat cggcgtcttc ccgacgctca tcgcgccgct
     9181 cgtgaagggc acggagactg tcgccctgca ccgtacctac ctgaccgagc aaggccgcaa
     9241 ggcacccgtc ccgacggtgc ggaagctgac gcccgcggct gggatcctct cgggttgcgc
     9301 gataccgctg tcggcgccat ccggcggggc gatcggcgtt gccgagggaa tcgagaccgc
     9361 actggccgcc tctcgtctct ccggcttgcc gaccgtagcg gcctatagcg cctccaatct
     9421 tgcgtcctac cgctggccgc gcgaagcacg acgcgtcatc gtctacggcg acaacgaccc
     9481 cgccgggttg gcggccgctg agggcctgcg gtcaagagcc gcggcggccg gcgtttcctg
     9541 ccaagtagtg atccctgaga aggctgggca ggactggtgc gatgtcctgc aggagggtcg
     9601 cgaatgagcg aacaagacgt tctgaacgct caggccgcag ctcgctccgg cgtcatcttg
     9661 accaacggct caaccctacg gcccgagcca gtgaactggc tgtgggaggg atggatcgcc
     9721 tgcggcaagt tccacatcct aggaggggcg ccaggccagg gaaagacgac gctcatgctg
     9781 gcgatggcag cggcgatcac ttgcggcggt cgctggcccg acggatcgcg cgccccgcag
     9841 ggcaacgtcg tggtctggtc gggggaggac gatcctcggg acacgctgct cccgcgcctg
     9901 ctggcgatga gcgccgaccc gtcccgcgtc tacttcctaa cggggatgcg tgagggcgac
     9961 gaagagcgca gcttcgatcc ctcgaccgac atgctccagc tcaccgcgga gatggagaag
    10021 atcggggacg tcaagctgct gatcatcgac ccaatcgtca gcgccgttca gggcgacgac
    10081 ttcaagaaca accaggttcg ccgctccctg aagccggtcg ttgatctcgc cgacgctacg
    10141 ggctgcgccg tggtcggcat cacgcacttc ggcaagtcca ccgccggcag agatccgacc
    10201 gagcgtctgg tgggcagcgt cggctacgcc gcgctggcac gggtcgtgat ggcggcagcc
    10261 aagtccaagc ccctgggcga cgaggccgaa cgccggatcc ttgtccggac gaagtcgaac
    10321 atcggcccgg acgatggcgg cttcgaatac cggctccgcc agacggagct ggatgcgttc
    10381 cccggcgtca ccgcctcgtg tgtcgagttc ggggcagcga tcgatggcac ggctcgtgat
    10441 cttctcgccg acgcggaagc cctgcccgac aacgacggcg acgtgcacct aaacgatgcc
    10501 gcggacttca tgcggaagat catgaccgac gtccttggga agtcggcagg cccgatggaa
    10561 agccgggaag tcgaccgcct gatgaaggcc gagggctaca ccacaaaggc catccgccgc
    10621 gctcgcgagt cgttgggcat cgtcatcaag cgcacaggtt tcggcgagca aacaaggacg
    10681 ttctggaccc tacccgacgg cccacttatg cccacttctg ccactcgtgc ccacgttccg
    10741 gagtgggcaa gagtgggcac gaatggggca cgagtggccg acgcatcaag gtttgacgac
    10801 acggtcacgc atgacgagga ggccttctga tgtcggcgcg caagctgctg aacagcatcc
    10861 atgcggcagg gatggcgatc gtcctggagg gagagcggct gatcgtccga cccgggtctt
    10921 tgctgacgag cgaccttcgg gaatcgatca cagcgatgaa gccggaactc gtccggctgc
    10981 tgcaagcatc gaactccccg gcgttcgagc gaccgcacgc ccttccgccc catgaactcg
    11041 accgagcaca tgctgatgcc tggggtgaag aggccatcgt ccggttcgtc gaccgcgtgg
    11101 gcgccctacg cggcgccggc ctcggcgaac aggacgccga agacctcgcc gaacgcctcc
    11161 acttgcgcga cctcgacggc gccgaccaaa gcgtctgcgt tgtggactgc aagcattaca
    11221 gcgtcgggtt gtgtcgcaac catcgagagg cgtcgatctc ccacgagcta ggcgccttcg
    11281 ccacgacgcc gcagcggtgc cccggcatcg cctggaaggc catccaatga tccgcaccgt
    11341 gcttggcctg gcaggaatcg cctgcgccat cgcagccacg atcgtgcttc cgcggtcttc
    11401 gctttggacg cggatgcagc caagggccgg cgcaagcccg accctcgaca agacccaggc
    11461 aacccatcga ccccgcttcg cgtgcaaggc acagcgcgaa gaccagtagg agcatctatg
    11521 aaccaactga ccatccgccg gcgcatcgcc cttaccgagg ggcagcgcaa ggcgatcaac
    11581 caggcagccc acgtactggc gcagcagtct ttcgggcgaa tccgcgacga gatgcttcga
    11641 aaggctcgcg ccatgcttga cgaagggcac agttctgacg aagtctccga ggcaatccgc
    11701 tcctggatcg tctttgacgc gtgggcagaa cgccgtgctg aagggggtgc gctatgagca
    11761 tcaaagcgcc cgcccccact gttcaggaag acgagcccgc cccgcggcgc accaggcgat
    11821 caaagccgga gcgcactgaa cagaccacgg cggtattggt gcaggcgtcg acactgctcg
    11881 cagaaccggc gtcagcagcc gttccgacga tgccgcagtt catgccctgg ggtgagtcgg
    11941 ccgaggactt caacagccgc atggctggcc tggccgatcg gctcgcgatg atcagcgggg
    12001 ccaagcgatg agccgccgca ttgagatcga agcctcggac gaagtgttga aggtgatcga
    12061 ccggctcggc cagcgctggg gcgagaagat ccggacaaag atcatcgaac gcgcggtgat
    12121 tacggccgag gacgcgctgg cacatgccga gcagcatccg gcctggacgc ctcctgatct
    12181 ccgcgggaac cgctacctgt ggcgctccgg cccccggtag caggcggcga ggcctcgacg
    12241 gggtctcgcc gcacgcactc agtggcaagt gaacgtgact tctacgttca tcggcgtcca
    12301 gcctcgagcg ccagtttcct cggcattcaa aacttccgct gtcttgttca tcccggcgca
    12361 atgcgcgttc gccagcttta cggcctcgaa cttgacctca gtccaactgt tcaggccacc
    12421 tcgctgctgg tagccaacca tgtatttgtc gcctccaagc gccatgactt cggtgtgcga
    12481 cgcgcatgcc gtcagcccga gagcaattcc gatagcaagg aaacggaaca ttctgacctc
    12541 ctccatatcg agacgcgtgc agcataccag tcacttcatc ggacaagacg acttgggcgg
    12601 gaagcgtagc gcattggcga cgtcacgacc cgggttttag aggtactaga ccacgatggg
    12661 tagcgcagtt gaatcacgtt atccggatta aatcgaattc actcgcgacg cgctgtaaat
    12721 acgtccatag tgaagcgatg gacacccatg catgcgacct ccaggctatc gagccccggc
    12781 atatcgaagc gcttctgacc gccgacgaaa ccgcacgtgt cttggacatc gatcccatga
    12841 ccctcgctgc gtggcggcat cagcggaggg ccctgccgtt cgtcaagatc ggtcgaatgg
    12901 tgcgctaccg ccagagcgat gtgcgcgcct ttatcgacgc gaacgtgcga gaggcggcgt
    12961 gatggacgac tttcttgcgc tcgccatcca gaactacgcc caagccgagc tcgcgcaggc
    13021 ccagcggaac ttcttagcga acgctcagtg cgttccagca gacggagcgc cgccgccgat
    13081 gcagcaacct gtgcgggagg ctgccgacgc gacgccggtg ctagtgcagg atgcgctcga
    13141 agcgctcggt acgcgctacg cgcagcagca cgccctcggg ggccttctgc gagctctgcg
    13201 caacgtaccg ggcgtgggcg acagcgtcgc cgccgcgctc gccgagggcc aggagacgca
    13261 gctcatcgcg aacgcctgcg acgcgaccag agatgcctac ttctgcgccg cagaagcgct
    13321 tctgtcggtg ctgatcgctc ccgaaaaagc gaggcttcag tgaccctgaa ggccccatac
    13381 gaagcggcgc tcagccgcca tctagtggat caacagcgtc gcgcccttgc cctggcgagg
    13441 ctcttcgttc gtccatcgca agccgcagga gattcgacag ctcctcaaag gtgtccacct
    13501 ggggctgatt cgtcgaagag ccaaggttga acgccgcccg gcgctcgaac tcctgctcaa
    13561 cttcatgccg aacctgatcg tcaacgccgg ctaccttgag gatcccgagc actgctcctt
    13621 cgatcgcctg gagctgaccg ccttggcgcg acatctcctc agccagcaag cggatggcgt
    13681 cctcaacctt cttcaaccga tcgtccatcc agcctgcctc tgaaccgatt gcaccgacac
    13741 agcgaactgt acagccatcg acatgcgcaa tttcctgaag atcgcctcca acgtccagac
    13801 gacgccgctg ctgctggcct tggcccgcca gccgttgctc tggaatgagc acacgcttcg
    13861 cacgcagtac ccaggtacgc cgcacagcga ggtggacgac atttggctgc gtttccaaac
    13921 cttacctgag cccgggcgcg aggtggaggt cgtggatcag catgagagcg tttggtatcc
    13981 ggcaatcaac gcgctgcccg aggtgcgacc gctggtcttc aacctgatgg cggctgttca
    14041 aggcgagcgc cttggccgcg ttatggtgac gcgtcttcgc gctggcggtc ggatctaccc
    14101 acacaccgac gacggagcgc acgctgagta ctacgagcgc ttccacatcg tcctggcaag
    14161 cggccagggc tgcaagttcc gggcaggcga tgagatcgtc cacatggcgg cgggcgaagt
    14221 ctggtggttt cagaacgcgc tcgagcacga ggtcatcaac gactcgaagg acgagcgcct
    14281 gcacctcatc gtggacatcc gatgcccgca aagccgtcaa tccccgggaa acggcggtgc
    14341 gtaaggctgg cgcctcggct tcctcccgtg gcgacgtctg ggggcaaggc agccgtcgtc
    14401 tgaatattgc gcttagcgac tcggtgtttg cggctgggta ttggtgggaa tcacagcgag
    14461 cccaaaccga ggggcgcccg ctgtgcgaag caactggtcg cgcatctgct cgattccgtc
    14521 tcgcatctcc tcgggcagcg tcgtggccaa gtgttgatca agcctggctc ggctcattcc
    14581 ttcgaagaat tgcaaaacgg cttctcggta tgggtgggtg gctatgagcg cagtaacagc
    14641 aatttcaagg gccatgatct ggccttcctg tcgcgacacc agcgccgcca gtcgttcgag
    14701 ttccatgggt gcccctttcg tggaggaatg gtctagcaac cgcaactctg ccacgactgc
    14761 gggcgcccgc ccagcctcct caggaactgc aaggtgaccg aacacagggg cccgacggcc
    14821 tacacagtcg agcgctggag cgacattcgc gaagagatgc tcccgctgtt gtaccggcac
    14881 tggcgagagg tggcgctgaa ccatgacgag gtgccgctgc acatcgacga gaagcgctac
    14941 gccgacctcg acgcgaagga tgcgctgcac atcgtgactg tacgtcgcga tggcggcttg
    15001 atcggctacc acgtcgcgtt cgtgtcgacc catcttcatt acgcgagcac gctccacggc
    15061 atcacggacg ttttctggat tgcaccggaa tgccgccacg gcgtgaccgc gctgcgcatg
    15121 ttccaaacgg cggagcgcga gctgaagaag ctgggcgtgc gcaagctctt cacggctacc
    15181 aagcttcatc tcgaccaggg ctcgcttttt gagcacatgg gataccgccc ggtcgagcgc
    15241 ctgtactcga aactgatctg aggactccac catggttgca gcagcaggca tcgcgtccgc
    15301 cgtcatcggc gcgggcggtg caatcgtcgc gtcgaacaag caggccggcg ccgccaacag
    15361 ggcgaccgac ctccaagggc agatctacca gcagaatcag gccaacttgg cgccgtacat
    15421 ggccgcgggc acgccggccc tatcgcggct gtctgatctc ctgggcacga gcggcaacac
    15481 cggcgcgagc ggctatggat cgctcacgaa gcccttcacc gtgcaggact atctgaacaa
    15541 tcaggatccg tcctatcaat ggatgcagca gcagggacaa caggcgctta tgagcagcgc
    15601 cggtgcccag aacggcgtcc tctccggcgc cgcgatgaag gacctcctga acttcagcca
    15661 gggcctcgcc gcgaatcagt accaagcggc ctacaaccgg tggacgacga accagggcaa
    15721 cacgttccaa aggctgtcga acctggccgg tctgggggaa aacgccgccg ctggcgctgg
    15781 caatatgggc gtgcagtctg gcgcgaacat ggcgaacacg atcaccggtg cgggcaatgc
    15841 ggcggcctcc ggcatcgtgg gagcatcgaa cgcggtcagc gggggcctca acaacatggc
    15901 cggctactac tacctgcagc acaacacggg caacccgctg atgtcgcagg gcttctacgg
    15961 caacgactcg acggggtaca cgaacgccgc cccgtggacg gttggcccgt cgcctgatgg
    16021 aggctcctga tgcccatcga tccgaacatc gctcttggca ttcagaccgg cagcccgttc
    16081 caaacggtcg cctcgatgct caacgtccag aacggcctcc agcagaatcg cctcctccaa
    16141 gcgcagaccc aggcggccaa catcgctaac caagcgaacg ccgccaacct cggtgagcgc
    16201 aacgccctgc gcaacctcat caccgatccg cgagtgctcg atgcgagcgg caacgtcgac
    16261 ccctcgaagt acgccgtcgt ggcgccgcag gtggccccta cgctgggcgg cgacgtctcg
    16321 aagtccatca ccggcgcgca gaccgcggcc gtgacgaacg ccgaaacggc gttcaagctc
    16381 aagggcgcac aagccgaccg cggcttccag gaggccgctg ccttgttcca ggatccgcgc
    16441 attcaagggc tgacggatca ggcttcgccg ggtcaggtca acgcggccgc gagtgccatc
    16501 ctcgaggccc aggatcgagc cacggctgct ggtgtgccgc gggagaccat ggctgtcctg
    16561 acggcgccgt tcctgaacgt gctgcacaac aacccggcca tgctgccgca ggcgctgaag
    16621 aatgtcgtcg tcgcaggcgc tcaacgcagc cagcagccca ccgtcatggg gccccagtat
    16681 tcgcccatct cgacccaagg tggcacgcag ctcgtgcaga cgaatgcgtt cgctccgggt
    16741 ggtgtcggga atgctggcaa tccctatccg ccgccgaatc agatcattcc gagccccggt
    16801 ggcccagtga tcacgaatac gtcgaacggt caggcccgtc cgctgacgcc tgcgccgtcg
    16861 acgccgccca tggcactgcg gatcccgagc ggcgaaacga cgcagacgga acaagctctg
    16921 caggaccaac gcacggcaac gcaaagccag ctcgccgatc tgccagctcg ggcagaggtc
    16981 tatcggaaca tcatcgacct cgcgaacaag ccgggcctgt cgaccggctc cttcgccgag
    17041 ggcgttcgaa acctgagttc gcgcactggg tacaactttt tcagcggcaa cgcggtcgac
    17101 tacaacgcgc tcggcaagta cctggcgcag gaagcggcgc ggacggcgtc ggcgatggga
    17161 ccgggcacga acgctggtct ggcgaatgcc caggcggcgt cgggctcgac gtcctatgac
    17221 ccgacgacta tcaagcgtat cgcctatctc tcggaggcga acgcggcggc gctaccggag
    17281 tacgacaagg gcctgtcggc cgcgatccaa gcaaggctcc agcagcagcc ggacactgat
    17341 ccggtcttcg ccaagcgcca gttcgaccag cagtgggccg cgacgtacga cccccgaatc
    17401 tatgagctgt ccaacgcgat gaagcgcggc gacggtgaac aggttgagga gatcttccgc
    17461 ggcctcggcg tgcctggcac tttcagcaac ggcaaattcc agccgtcagg ccctatgacc
    17521 accgcggcga ccgcgctgtt ctcgaaggcc cagcgcctga aggcgatgac cgcaggggcg
    17581 gcgcaatgag cgacaccgtc gactacgatt cggtgcttgg gctgttcacg cctcccgacg
    17641 cggacaagca gatcagcaac gcctcggcga tcctcggctc gctcaagggg cagtcgcccc
    17701 aggtggtgag caggacggcc gctgccctgg cgagcacgac gacgcatcca gccgtggccg
    17761 ctgcagcgtc ccagctccag tcggatgcca acacgacgat gaccacgcag acgactcgtg
    17821 gacacctcgt gcagcatccg gacacgaacg cggctgtcga tcgtgcccgt tcagcgctga
    17881 gcccggcttc ggtcgcgttc acgccgcagg agcaggccac ctccgatgcc atggagcgca
    17941 acccgtgggc gaccaacggt ttcgctcggg cgtatgcctc tgcgtcgccc caagaccgtg
    18001 ccgacatgct gcgctctcgg acgatggtca tgccgtcgaa cgtcgactat gacggcgtga
    18061 tgggcctttt ccagggcaag ccggcgccca gcaacggcat gcctgcgatg cccacgaccc
    18121 ctcagtccct gcaggtcgac caagtgaagc cgggcagcga cccgtactcg gacgcgctct
    18181 atgccaagac aggctctggc gactacacgc gtcctggcga gacctattgg caagcctatg
    18241 gtcgcaacgc cgcctatgac caggcgatgc gcaacgctgg tcttcggaag ccggcgagcc
    18301 tgctggattt ggcaggcaat gccgtgtccg gcgtcgctgg agcaatcgct ggcgggtatc
    18361 acggtctgct cagcctcggc caaggcacat cgacggcagg aacgaacgcg gagaacactg
    18421 cgcaggcgct gacgtaccag ccgaccaatc cggagtcgag ggctgtcctg gacatcgtca
    18481 actcgaacgc caaccccctc aactggccgg gtgcggtcgg gcactggatc ggtgacaagt
    18541 tggcagagca cgggatgcct gccgttggtg ccgccgcggc caccctgggc gccgccgcgc
    18601 ccctggtact cggcgcaagt tcgaaggcac ggaacgcggt cgcaagtgct ggccgcagcg
    18661 ctgggaccac ggtcgcagac ctggtgcgac ctgctgcacc ggccgcgccg cgcgtcgagc
    18721 caacgttgga gccaacgggc gtacagcagt cttcagggag cccggcaagc actgctgctg
    18781 cgccggcggg agcccgtacg caggctcagc cgaatcaagc cgctccatcg ccagggcagg
    18841 ccgccgggcc gcccggctac ttggatccaa ccctacgtgt gccgagcgct cctgctgccc
    18901 cgcgcgctcc ggcgctgagt gatggtgcta acggcgtgtt tgccgaggcg ccgactcccg
    18961 cgcaggcttc gctgccgatc gaggagcagc agcgacgtgc ccaggtgctg aagagcatcg
    19021 gccttgacaa cgcacgggag agcgcgacct ccggtgacgc gaagcaggcc gcgacggact
    19081 ttcaggtctc gcgcacggac acgccaggcg ggaacgcgat gtcgcaggtc ttcgcccggg
    19141 agcgccaggc tttgaacgac tacgcggaca acatcgtcaa ggacaccggc ggcacgacca
    19201 tgaatgacca gtcggccaag tacagccgcg gcgagacaat catggcgccg ctcgaggctc
    19261 tgaaagacta ctacgactcc aacatcgcgc ggctgtacgg cgaggccgac gagcgggctc
    19321 agggcgtgcc gacgcagctc gatcagttcc ggacaacgct cggcgacgac tcgctcatga
    19381 ccaacagcga ccgcgttcag ctccgcagcg cgatcacggc gtacgcgaag aagcttggga
    19441 tcatcggcga caacggctcc atctttgcga acggccagca ggcggagacg atgcgcaagt
    19501 acctgaacga gcagtggacg ccgcaggctt cgggactgat cggtaagctg aaggatgcgc
    19561 tcgacgacga cgtgatgcag tcggccgggg aggacgtgta tggacaggcg cgtgaacttc
    19621 gctcgcagcg cgctcgggtg cttgacgatc ccaacggcat cgcgaagctg atggatgcca
    19681 gcggcccgaa cggcataaac cgcaaggtgc ccgtcgagaa ggttgcggac acaatcacca
    19741 gcttgccggc cgaccagctc gggcacatcg tgacgacgct gaagacggtt cccgatgccc
    19801 tacagccaca ggctcaggcc gcgctggccg agatcaaggc gcagttcgcg cacaacgtgg
    19861 ccgaaaccgg gggcaagact gcaggccagt ggaacaacaa gggcgttcaa cgctacctct
    19921 cgggcaactc tgcgcgcctc ggcatcgtgt tctctcccga cgagatgcag cgattcggca
    19981 cgctgaacga cgccggcaac atcctggcgc acagccaggc gtatccgggt gccgcggcgc
    20041 aggcccacaa cctcgtcaac gctggcacgg gagctcgcgc ggtcgggctg gggctgcaga
    20101 gtgccggcgg cgcaatcggc ggcacattgg gctccgtttt cgggccggta ggcactgcgg
    20161 gcgggacaac gcttggcgcg gcccttggcg gcaaaggggc cgggatgatg aacgacgcgc
    20221 tcagcatgca cgcgttcaac aagcgcatgg tcaagctcgc tgatttgttg ccgacctcga
    20281 agcagagcgg cttgtcagac gtgggacgga ctcgttaagc aacgggctgc accaaacggg
    20341 cgcatctttg aaccgcaggc atctttggac tgtggatcgc gttcagcgac gccacatctc
    20401 gtgagggcag caatgtatca gtcaaacagg gcgctcttgg cgcccgagct ctatgtgctt
    20461 cacccggacg ggtctgcgac ggttgccacg ccgatgcagg tcgcgctccg catcgccgag
    20521 ctattggcga agacttcggt catccctgat gagcgaccag cagttgccag cgcacactgc
    20581 cctgggtgta ggtatgctga tgatgattct gcggcgggag acgcgtgcca ccaatgtgcg
    20641 tgatttcttt accgcatccc gggcaccgat agatgccgga caccgcaggc accgtaccgg
    20701 ggttccagac tacgtcgaac tcacctcctt gatgctgggc caggaagttt ccgtacttgt
    20761 agaaagccat ccagtttcct cctcagtgcc cggaaccgcc gggcgcgctt ctcgacgttc
    20821 tttgcccagc gtgctgccga tgcgccaccg ggcgtctcag cagcgtcctt gggtggcggc
    20881 attcggcaac gcagtgtctt ttgagcgacg cccctatcct caaaacgtat tcagcaccca
    20941 aaacccgaca tcgcctcctc gaattggtgt atggcaccgg atgggcatcc agcgccctag
    21001 accatgcacc gccgccgcgg atctgggccc cgggcatcca cgtagtggaa gaaatccgag
    21061 cgctggtcga caatcggcgc aggggatagg aggagcccat gacccgtttt cgccagatct
    21121 cggcactaag cgccgcctct ctgcttagca ttcttcccgg agcatctcgg gcggacttcg
    21181 aggatgatat tagcgactac gtcggatata ccatcatcgc cgtcaagacg atcgactcgt
    21241 tcaaggacaa cagtcacgag cgaaaggacg gcttcgaggg ttgcgaatac gacagggtca
    21301 ttgtcttcac tgatggaacg gccgttacat gccgcagcta cggctataag tatgcgtacc
    21361 gcccgaaagc ggtcatcctt gggcagcaga tggcatttca agggcgggcg gtgacgatcc
    21421 tcaaaatggt ggttcaaggc acggcatacg acgtagcctc gcgctaaacc tctcgcggag
    21481 ccggtcgcag tgtcagcgtc gagccaaccg tattggcaga acgttgtcgg ctgtcagggg
    21541 tcaagaggtg agcgtcaaag tagcgcttga cggttcgcat gccgtacagg cgtttttgct
    21601 tccgctaccg ctcgccggcg cgcaggcttc cagccaacag ccggtttgta cgctcaaccg
    21661 cgaagggtcg gctaccatcc cagcgatgtc aaagccgctg tccatcattc tggcgactgc
    21721 cgccctgtgc ttggcaccgc ccgcaaccgc atgggcggac gaccgtgccg cctgtgcgga
    21781 ggccgatgga agccttctgg aaggcgtcgt cattgctcgg ccccaatacg ccttgggcaa
    21841 gccgctccgg ggcattcccc tcagccacac gcatatcaag ctccgcggcg accaagacgg
    21901 caagacctac gacgtcgcgg tggacaacgt attcgctgcg ggttatgacc cgaacgtcga
    21961 ggccgttccg gcgccgctga gcacgatcac gccgggccag cacctcgagg cttgcggcat
    22021 cccgtacgcc ggcggcatgc actgggtgca cacgaactgc ggtgccacgc cgacgccgca
    22081 ggatccgaac ggttggctga acgtgctcga tgcagcaggc aacgcggggc cgaacctcga
    22141 agacagccag aagtactgct acctgtggcc tgccagagct cacatgcggc accgtcggca
    22201 tgctcagcat tcggtggaat gaggaaccac caaatcgtga gcttgcgcca cggtgtgttg
    22261 ccttagggag ccgtgcagtg gcctggcgga cggcgggtga cgggcctatt gctgcggggg
    22321 cgccagtcac cgccaccgac agcagccgac ccatcagcga cattcaaagg cgggaatctg
    22381 ggcgccggaa agctgtcgct caaccgcccc tgaactgctt ctcgtaggca gcgacgtgcg
    22441 ccggaaatag ttcgccgtac agcgggtcgg gtatgcctag taccgttgcc tcgacatagg
    22501 gctcgtcgcc gacgtctgcg tgagccgcgt agtccaagac actcaccccg gagcagggca
    22561 acttgtcgcc ggccgaagtc ctggccgtca acgcgagtgc cgcttggtcc gtgtgaatca
    22621 tccgacactc gtcctgaatg agtcgatagc cttccgtcgg gatgaacttg ccgaacgcga
    22681 cgcccattgg cggatcgccg aattccagcg cggagtgccc gacgagggtg tcgcctgaga
    22741 aaactgcaaa tctcatttct gaagctgccc ttcaccaaag cccgcttgtg gccgaaagcg
    22801 ccttcacttt catcgcaagt cactttcttg gtagtgaaac accgaggcgc gctcgccggc
    22861 taccagccag tcccttacgg tatgcctatc cgccgtaggc agaacgtcga cactcagcat
    22921 ttccgctagc ccgaacgaga gctcgatttc tccgctctcg tatgtgcatc caagcgtctc
    22981 tgcctccggc caacagtcgg cgtaaaaagc ggccttcgac tcaatcggca caagcaatat
    23041 caacgttgaa tgcccagact tctccaccac ctcgtcgaaa acatattccc cgtcctcatc
    23101 cctcaggacc gacactacgt ccaagaagga aacgcctttc gcaaaaaacg gtgagttctg
    23161 aacttgaaac aaagtcaagc gatcgctacc gaggacctct tttgcccaca tgcgctccaa
    23221 ggagtggccg tgccaatcct cagaatccaa cttaaacctg actttctcca ttcgaggtgc
    23281 tctcgttcct gttagcgcct gcagcctacc cgaaaaccga cgctcacgaa gggcagcaag
    23341 tggccggcag cacgccgacg gtgatcgcca tcgtacggca gttcgccgcc tggtagcata
    23401 ctttcgtcct cggaacggct ggttgaaggc agccagccgc cgctcgcgcg gcgtcgcgct
    23461 tcagccgagc ccgacccaac tacgacattc aggcgcctcg ttgaatcgcc tcaaagctgc
    23521 catccgagat cgccgctcga ggctactcaa cactacggcc gggtaagtgc ctccaaggac
    23581 tctgcccgcc tttcggtcaa atccgccttg caatcggcga cgacagcaat cggcatggtt
    23641 ccacccaact gcgactccgc cccctcaaac tcacattgag catcacggaa gccgagccag
    23701 gctcgttctg ccttgatcaa cagggcgtct gtgtgctcgt tcttggtcgt ctgaaggagt
    23761 ctagagtaaa catggttcaa gcggagatcc tgctctgtaa ggcgataaga cgcgcagatt
    23821 cgcaacgtca agttatccct gtcacattgt cgaaagcttt cccgaagcag ctgctcaggc
    23881 agactatgag acgcccggag catctcctga atgacccttt cgcttggatc cggaattttc
    23941 tgatctatta agccgcccga atcatcatcg ccctgacctt ccgcatgcac gcagatggac
    24001 acgatcgata aagcaatcga caaaatgagc tgacgcatga tcatctgatt tggtgacgtt
    24061 gcacgatcct agctggctca acaacggtcg ttcgcgagcg gccgcaagtg gccggcagct
    24121 cgcttgcctc ggcgctgatc gtacgacagt tcaggggcag caagccgtcg gacaggccgt
    24181 aagagcccgc ccaatgagtg gcagctccgt ccgcaggttc tacgcgtcgc gaagatggcg
    24241 tgcggcacat ggcgcattgc acctccgcga ccggcttcgc tatcgctgca cgggcgagta
    24301 attgagcagg gatgctgcgg ggctgtgctc atacagacgt gatgacatcg cggaacgcgt
    24361 cgatcgccgc gtgcgcaaga cacgttggtc cgaggttgcg cgcacggcgc gccagcagcg
    24421 tcatgggcca gcctacgccg acatgaaacg cgagcgtcga agccatcgag tgtatcgagc
    24481 cgtggccgaa cggcagcagc gggaagtgcc atgcgcccca gagcagcgaa acaaagactg
    24541 cagttgcgtg cgcgcgcagg ccggtcaccg gtgcatccgg gacgatggcc ggatcgattg
    24601 ctcctcggaa gacgacctct tcaagcacga agcagacgca ggcatagagc cacgcgtcgc
    24661 tgatgaaggt tgcgagcccg gtcgcaggtg gcaagtgcgg ccgggcgcct gacatccacg
    24721 cggagagtgc gaagagcatg acgccggtca ccacgaccag ggcgaagaat gctggctgct
    24781 ggcctcctcg ccagaactga ggttgacgcc aggcgttgac gagccaccac gctaagccga
    24841 atgctccgac cgcagcgcac aagcaccacg ctgcaacgtc gaccccgacg cgggccgacg
    24901 tgacgaacga ggccaccagc gagagcgccg gccatgttgc gagcatcaag ccgacggtca
    24961 cgatcgatcg gtcgaatcca agtggcgcgc cgtcgaagcg ccaaagcgcg cgcaccggtc
    25021 gatggaggat gaccagttgg tacgcgagca ccagaggcac ccccaggaca aggtacgcgt
    25081 tcgggggtag cgccagcagc cagccagcgg ccatccatgc gccgatgaag acagaggcca
    25141 gtgctgcctg cagacgcatc gaaggcggcg cttccctgga cgccaggatc atgggctccg
    25201 tcaggcctct tgtttggcgg ctgtgttgac cgcggccttc cggacctccg tcaggatctt
    25261 cctgcgctga gccgcgggca gctttgcgaa ggccagcagg agctccgcgt gctcgttctt
    25321 ctcggcatag aagaaggggg tcggtacccc aagcacgcgg gccagcttgc gcactagggt
    25381 cgcgggcggc tcgtgggtct cgcgttcgta ctggttcatc cgggcgctcg acgagcccgg
    25441 cgcgattccc gccaggatgc cgagctgcct ctgcgaaagg cctgccagct ggcgcgcttg
    25501 cttcaggcga gcaccaaagg tcgacatcgc gtcgtctcga gattgggtgg tggcgatcgt
    25561 acgcgctggc gaggcgccgc gggtcagtag ctcggaacga acggctgggc ctgggcgtag
    25621 tttctcgcga agtgctcaag gtagacgctc gcgagcttcg ggttcgacca gttgaccagg
    25681 acgttctcgc tgttggcgtg ggcggccgcg gcgctgtagt tgaagctgcc aagctccacg
    25741 gtctgaccgt cggcgatcac gaccttgtcg tgatggatcg agtaaacgct gatcgtgcga
    25801 acgtcggcgc cggccgcctg cagggcgccg agggcggccc gggccttgcc gctgcggtcc
    25861 tggctgatgt tctccttctg gtcggcgatc acgcggacgt cgacgccgcg gtgccgggcg
    25921 cgcacgaggg cctcggtgac agggacgctg gtgaagctgt aggcgagcac ccgcagctca
    25981 cgctgggcgc tgtcaatgac cttgaccacg aggtgttccg agccttcatg cggactgaag
    26041 gcgacctcga tcgacccggc gcaggaatga tgtaagagtg gctgtcctgg taggcggtga
    26101 cggcctggtc gatcagcgac ggatggcgct tgctggcata gacggactgg gctgtggcgg
    26161 cgagggctgc ggcgagagcg agacggtaga ctttggacat ggccggcatt gtcgtgctgg
    26221 ccgcggccgg gacacacact cgtcgaggta tccatgtgct acagcgcaca ggtctacgcg
    26281 gactggaaaa agtacacgcg gatgttcggg gcgaccatga gcgtcggcga gttctaccgc
    26341 accttctggc ggcggaacaa cgagcccgag gccaaggtga agatcccgaa ggcgatggag
    26401 catgccttcg ccgatcctca gaccgacgac gagcggcgga tcaaggccga gatcgacgcg
    26461 tggcacgcca gcgaagcgac gaggctcgag caagagctgt tcgcccagcg caagcgtctg
    26521 gcggacgcgg agcgtgttct cgccggcccc aagcccacca agaaggcggc ggacgacgcg
    26581 cgcatcgccc ccgacaagat cgaccgcgca cgccgcgcgc tggccgacct gcagcgcact
    26641 gagctcgagc cgcgggactc tcgcatcttc cctggcgtct acgcgcccgt gatggtcgtc
    26701 caggacggtg agctcgtcgt tatgccgatg cgttaccagt gccgcccgtg cggctcgccg
    26761 gcgttcttcg acacgaagtt ccccggcacg tacaacgcac gccgcgacaa cctgggaggc
    26821 aagttctggc agtccctgtt cggcttctcg cacgggatca tgctggccag ctcgttcttc
    26881 gagcacgtac ggcgacacaa ggccgagggc cgcgagctgg gcgacggcga ggccgaagag
    26941 gacatgatcc tcgagttcaa gccggccggc gtcgaggaga tgctcgtcgc gtgcctctgg
    27001 gcgcgctgga cgtcgccggg gcagccggac ttgctgtcct tcgcagcgat caccgacacc
    27061 cccccgccgg aggtcgcggc cgcggggcac gaccgctgca tcatcccgat caagcgcggg
    27121 aacgtggacg cctggctaaa tccggacgcg tcgcagctcg acgcgttgca tgccatcctc
    27181 gacgaccggg agcgtccgtt ctacgagcac cgactcgcgg cgtagcggcg ggtcaagaac
    27241 gcgccctgcc gggccatcag gtcgtcggcg atgcgccagc aggtgtccag tgacagctcg
    27301 gccggggcga agtggtcgtc caggggctgc ttggcggcag cgcgcgcagc tttgcgggcg
    27361 gcttcgagcg cgcgctgctc gtcctggtgc cgccattcct cacgggacgc ggcgcgcata
    27421 gcctccaggc gggcgatggg gacatggttg gcgctgcgct gaaatcccgc tggcgtccag
    27481 tcgaccggaa ggtccagacc cgggcagcgc acccagaacg cgcagccagt ctccggccga
    27541 gcggaggccg cgcggatgtg gccgtccttc acgatgtccc gggcgcagcc gccgatgccg
    27601 cccgagtcgc cgcaccagtg ctcgcagcgc cagcaggcgc ggctgaagtc gttgggttga
    27661 ggggtccaat gggaggtgcc ggagaacatg ccgctatgct ggcaagccgc aggctcatga
    27721 aatgcgccat cgcccgcaca cctcctaggt gtcgttcagc cgtcggacca gaccctggtt
    27781 tggtgggcct tgatcaaccc aagcagttcc atggagcagc tcaagaggta tcgagtggtg
    27841 ccctcgcgat ccggagtagg cgccgttggc gtgtcgatgt atgcacgcag ttcaccgttg
    27901 tagtcggcta gtctgtcgtg caggtactca aacttcttca gtacatcctc gagcagctgg
    27961 cgatcaatca aggcgagggt ctggaaggcg acttcgaagc ggccgagttc aagcacctcc
    28021 ttcgaccaaa atgcattcgc ttcctcggtc gttgcgccga ccgagggcag ccgcgcgcac
    28081 agctcattga tgtgctcaag gaatggctcg atcgataccg gtgaaaccac cttccgaagc
    28141 caaaagtcgt cttggatgga ctgcttgcgc gctcgcctgt ccttctcttg gttgtaggcg
    28201 aatgtcttcc aagacagtag aagtgccacc acgctgagcg cgactgacgg tagcgacgaa
    28261 acgaatttgg atgcgatcga ctcaggagct ggctggcaca cggccaccgt cacgctggct
    28321 gatggctgca tcgctgagca acctgttggc aactgcgcca tcagcgtcgc aagggtgtca
    28381 ctagcggttg aggccggagt cgatgcggca ggctgtagag acgcaaactg ccctgcgggt
    28441 gccgcagtag atgtgggcgg actcgttcgg agcgacgaat ggtgactcac gtaaacaggt
    28501 tgcgcgcctg caaagcgcgc gaaacgtagc catcgagcgc ctcgcggatg aacctcggag
    28561 actggaagtg gtactgctcg tagaaccgct gcttgctgcc tgctttgact acgctaggcc
    28621 cgaacatccc gaggaagaac gaactactga tcgcgtagat gttctcgggg atccgaacgt
    28681 ccatgacgtc cccggccgca tcgtgacgat caagctgcat ctcctcacgc agacgctcac
    28741 cgcggttgcg acccgtgaaa acgggccctt cgatcttggc gaagtcgatc gttcggtctt
    28801 ccaggttcat actgcttctc ccggcgtggc ttcagtgcta gccgtggtag agagggggaa
    28861 ttttaggcca atcagagttc ccgggaactt gacgccgtgc agatgccgca aaaatctgtc
    28921 gtcgggcttc atccgcaggt cattgaaggt gttgaaggcg atgatccggt tcccggctgc
    28981 gttcgcctcc atccggtacg tgccatcgaa cagggcatac gtcgagcccg atagaatgac
    29041 catcctcgcg ccaggggact gtccaccctt gcattcctca tgcactcgtt caaaaaaact
    29101 gatgagatcg acggtgccgt tccctctggt gtcctcggga cgagtgtttt tggtgctcac
    29161 gcatccttga agggcgatca acgtaaacag gccctctctg gtgccaccct ttcgaaacag
    29221 gccagtcttc tcgtgaaggt cgaggtacgt ctgaacatgc tggcgcgtgt agctgcgctc
    29281 gggcaagtcc tcaaacgaag cagcgatcgt cctgccgaag ttgaaaatca ctacttcaca
    29341 catgggcacg tccagctggg tgtcaaggta gccttgaatc gcccagtcgt acatccctga
    29401 gtgttcctcc gcgttatcga tgatctcccc gacgtacagg cagagctcgg ccttcgcgat
    29461 gtcgacaagc ctacgcccga tcgttgccaa gcactcatca atatgatcgg caaaggcggc
    29521 ggttacctga gcattgcggg tcgcctcacg gggccgcgcg gccctcacat agtgcttgca
    29581 cctggcatcg aacacacgta ggcgcccctc ttcctctttg gtcgggtatt catgagcgat
    29641 ccgcagtcgc cttatgacgc ccatggcgcg aatgaaccgc cggtgagccg ggtcggccgg
    29701 ataccgcccg tgccaccgga tgcttctccc ggtctgcctt gcttgcgtgg ctagctcatc
    29761 aactaggacg tccagtaggg cattcgcgcc aaggtcgtac tgggtgacct ttccgaaatc
    29821 caagaacagg ctgcccaggc gcttcgacct catttcgtga gcaagctggt tgatggcgcc
    29881 gagcgtgcga accgggtcgt cgatgatgga gagcgtcgac ggcagcacga agcggaggcg
    29941 cccgcccctc ttggcttcgg gccgtcgtcg gacgccgaac tgcttcttgg ctggagcatg
    30001 ctcaacggcc agcatttcga caatctttga cgtcttgccg aagtgcgccc tgagctcgtc
    30061 ttcgctttct aaccgttcgc gtttcgcctt ccaaaggcga tgctgcctac gctcgagttt
    30121 ccgtccacgc ttctccttca ggtagcgtgg tacatcgatc ttcgccaggt gaatcgccac
    30181 ctcacctcct cccgcctcag gtgcttgttc tgaattcgtt agcgaatgga ttttaggttg
    30241 ctcgacatga aggccgtgtg cagcgttttc gcaacgacca gtcagcggcg acttgcttcg
    30301 gccgccgttg agccagtcct caaggcggat cttgacccac caggccgcgg ccatcagcgg
    30361 cagccacgcg aggtcctcga tcgcctcggg cacggcttag cgccagccgg tgtggaagcg
    30421 cgagaagtac gaggccacgt aggccaggcc ggcgaccgcg aatgcaagga cggccgcggc
    30481 gacacaggcc gcaatcaaac cagcccgcgc tcgctggcgt tcttcactca tcggattcca
    30541 caggcgtcca gcccgcctcc ctcagccatc cgtcgacggt cgtccatccg tacctctcgc
    30601 gcttcccgcc gggcttgtcc ttgagcatag ccgcaatgtg ccaagccact cgtgccctac
    30661 tcatgcccac tcgtgcccat tcaagaccaa gggcataagt gcggcggagg gcatctgtgt
    30721 gctgttttta caacgtagca gcgttttccg acgcctctac gcgcgaggcg cctggaggcg
    30781 gtcgacgcca ggaggcacgc atcgggtgtg cgcgcgggcg ctcgcgcgcg aggacttgag
    30841 ttgccagcga tgacagtccg cctgtcacag gctgtgatgg gcgcaggttg actcgctgcc
    30901 ctcgccgaaa cctaaccacg ctggtaggtt tgcggttcct cggccctctt ccgcttggcg
    30961 atcccccggg gatcaaaatc gatgccccga gtttgaacgg ccgagaccgc caccgacgtc
    31021 aggtacccct ctgcggcgag aaattccatc ccactagctc ctcagatgac ctgatcacac
    31081 ggttggatga ggtagccggt caaacgcggc gaccaacaaa gagtcgctga ctccgattct
    31141 ttgctttggg ggtaacacgc tgatacggcc aagtctgagc agtacacgcg ggcgcacgta
    31201 cgcgcgaggg atacgccggc ccagcgccga tcgagcacat tccacgcacc tacgcccgcg
    31261 tgtgagcggg gcgagacgcc ccaccgctca tccttgacgc ctacgcatgt gcgcgagagg
    31321 cggccggagg tccctcccgg attactccaa gcctcagtgc gagagcggcg atctcgagga
    31381 aatgcaacca gtacgcgcag gcgcgcgcgc tcgcgcgagg catcgaggcg ccacaagcac
    31441 caaccctacg cggcaaggcg cctgagtagg tctgccaccc atgctgcgct ctcggctgat
    31501 gcgtcgcggg gccactgcga ccgcacgacg atcgcaccgc tgcaaacctc caggctgaac
    31561 gttcccccgt ggccttgcga cgtcgctgac gtctgtgcag ccgcttggct cgcctgcggc
    31621 cggctcggcg acctcctcaa gggatcgccc ggcttggatc gcagcgtgat ctcgggatcg
    31681 tcttccgtca tgggaagcgg agttttctcg gcgggcttgg cagaaccgac cttcgcccag
    31741 tagcctcgag gcggcacagg aacttcgtgc ctccggcaca gctttctcag ggccgtgtcg
    31801 gacataccga gctgggccgc caagtgggcc ggcgcctggc tccagacagc ggcgtacagc
    31861 tcactccgct tcatcgatgc tctccggcgt gcggctggcg atccagcgct cgacatcctc
    31921 gacccgccag gccgtgatac gcgacgagag ccgcaccggc ttgggaaagt tgccctcgcg
    31981 aacccagcgc caaagcgtgg cgggcgaaac cgggatcacg ttgggtacga gctgcgcctc
    32041 tcggaaaaag gcgctgggaa cggctggatc ggtcatgaag tgcaggtgca gtgaagacac
    32101 ttcatgatct ccagcgacac cgcgcgcgaa tagcctgcgg tgggctgcgc cacgcatcga
    32161 atgggatgcg ctactttgcg agtggcctgc gtcgtctcgg gccaggccgg atgccgtccg
    32221 ggcgaaggat ggtgaacatg gcctgcctga ccttctcaga gatccccttc gcctcgagaa
    32281 ggggcgcgat cacttcagcc ttgggcaccg tgccttcatc atcaggatcg tgcgggccgc
    32341 cttcggaggt caggcgccag tgctcgccta tttccgcgag ctcccgcagc aactgcgtct
    32401 catgcgaacc ccaaggccac cgcggcccgc tggcggcaac cgaacgtgca ggccagccat
    32461 cgaccaccgg caggttcacg cgaagcgacc acgcgtgaaa gtccgagacg gcgacccgcc
    32521 tgtcccagta atcgtcgttc gcccaccgat cgttcgacgt tccattcctc gtgctgtgtg
    32581 gatcgacgtg ttccttgagc tggccgcctg cgaacgcttc ggaggcactt cggaggttct
    32641 tcgcgagacg ccgaagcgga tcgtctggat caatcaccgg tggcggatga acgggcacgc
    32701 tgtcgaagaa cgcaaccgtg aggaaggtgc ttcgaacccc gtggaggccg atgcccaggc
    32761 tatccggatc aagcgcgctg tgcagcgcca cgagctgcca aagttcgagt gcctcttggc
    32821 ccgcccactt cgcccaggtc acttcatcgg cggcatacag gcctgaccgc caatgaccag
    32881 tcctcgccgc catcaacgcg tcgatcccgg tgaatcgacg caggttcgga tcgacaggca
    32941 cctctctaac cgcctaccgt atgccgcccc gagcgcggca acgtctcccg cccctcgccg
    33001 tcctaaaggt aacgtaacgg caacggcagg gtcgatctac cgcagaaggt tagcagtcgc
    33061 tatcacgcaa agacgcatga atttggtagg ccgtgttgga cttgaaccaa cgaccaaagg
    33121 attatgagtc ctctgctcta accgactgag ctaacggccc ggagccggcg attgtaggcg
    33181 gcgggtcagc gttgggagag ggcgttgccg accagcttgg aggtgatgtc cacgatgcgg
    33241 atcatgcggt cgtagggcat gcgggtcggg ccgatgacgc ccagggtgcc gacgacgcgg
    33301 ccgtccacct cgtagggcgc ggagacgacc gagagcgcct cgaacgggac gacgcggctc
    33361 tcgccgccga tgaagatgcg cacgccctcg gcgcggctcg acccctcgag caggcgcatc
    33421 agctgggtct tctgctcgaa gacgtcgaac atgtcgcgca ggctgcgcat gtcgtggtgc
    33481 aggtcctgca cgcccagcag gttgcgctcg ccgacgacga cgacctgctc ctcgctctcg
    33541 gcgatcgcct cgctgccggc ctgcacggcg gcgcgcatga ggccggcgat gtcgtcgctg
    33601 agggactgca gctcggtctt cagtcgctcg cggatgtcct cgatcgacag gccgccgtaa
    33661 tgcgcgttga cgaagttggt cgcggcgacg agctgcgcct ggtcgtggtc ctgcgcggtg
    33721 atgatgacgc ggttctggac gtcgccgtcg ggcgcgacga ggatcacgag cacgcgccgc
    33781 tcgcccaggc gcaggaactc gatgtggcgg aagatgctcg cgcgccgcgg cgcggtgatc
    33841 acgccgacga agctcgagag gttggacagc atcgacgccg cctgggcgat gacgcgctgg
    33901 ggctggtcgg gctgcagggc ctcgcggccg gcgccgagct cggtgagctc gctgagcagc
    33961 tgcgacgcgt cgtcggcctc gagcgcacgc gaggtcagca tcgtgtcgac gaacagccgg
    34021 tagccgcgcg cggtcggcac gcggccggcc gaggtgtggg ggctggcgat caggccgagc
    34081 tcctcgaggt cggccatcac gttgcggatc gtggcgggcg acagctcgag cccggaggcg
    34141 cgcgacaggg tgcgcgagcc caccggctgg ccgtccgcga tgtagcgttc gaccagggtc
    34201 ttcagcaggg tcttggcgcg ctcgtcgagc atggggcgat tgtaggagct tcgagccgcc
    34261 gttctcggca atcagccggc ccgcggcgtg aacgacgccc ggcaaaatcg ccccgcaaac
    34321 cccgcggtcc gcgcctgccg gcggcaaaaa ccctgtggtg taatccgtcg catgtcgacc
    34381 cccttccgtc acgtggcgct cttcggcaag taccaggcgg aaggcgtgcg tcccatcctc
    34441 gaggagatcg cccagttcct cgtgcgcgcg ggtgtcgagg tgtcggtcga ccgcgcgacc
    34501 gcgctgaaca cgggctggaa cgacttcggc gcgatggaga tcgacgagct cgcgcaggtc
    34561 tgcgacctcg cggtggtggt cggcggcgac ggcacgatgc tgggcatcgg ccgcgagctc
    34621 gcgcgccacg gcatcccgct gatcggcatc aaccagggcc ggctcggctt catcaccgac
    34681 atcccccacg cggactggcg cgacgcgctg gtgccgatga tccgcggcga ctacgaggcc
    34741 gagagccggc cgatgatcga gggcggcgtc tggcgcgacg gccagcagat cttccaggga
    34801 ttcgcgctca acgacgtggt cgtgcgcagc gccaatccga gcatgatcga ggtgcgggtg
    34861 cacgtcggct cggagtacgt cgccaacatc cgctcggacg gcgtgatcgt cgcctcgccg
    34921 acgggctcga cggcctatgc gctgtcggcc ggcgggccga tcctgcaccc cggcctcggc
    34981 ggctgggcga tggtgccggt ggcctcgcac accctgtcca accggccgat cgtgctgccc
    35041 gacgatcgcg aggtcacgct ggagctggtg cagggcaagg aggcgagcgc gaacttcgac
    35101 atgcacggcc tcgcgagcat gctgctgggc gacaccgtgc gcatgcagcg ctcggcgcac
    35161 aaggcgcgct tcctgcaccc gctcggctgg agctactacg ccacgctgcg caacaagctg
    35221 cactggcacg agggcacgac ctgaacggag ccgtcacgcc atggcgcgaa catcgacggg
    35281 ccggcgccgc gcgggcgcgc aggctcaccg gatgagccgg tgccgcggca tcgtggcgcg
    35341 gcccgcgcga tccgcgcccg ccatccgccg ccatcctttc ctgccgacac catgctgcgc
    35401 cgcctctcgc tgaaggactt cgtcatcgtc gatgcgctcg aactcgagtt cgacgccggc
    35461 ttttccgtgc tcaccggcga gaccggggcg ggcaagtcga tcctgatcga cgcgctgcag
    35521 ctcgcgctgg gcgcgcgcgc ggagggcggg gtcgtgcgcg agggctgccc gcgcagcgag
    35581 atcacagcgg aattcgattg cccgcccgcg ctgcaaccct ggctggacga agccggcttc
    35641 gcgctcgacg agacgctgct gctgcgccgc gtcgtcgatg cccagggcaa gagccgggcg
    35701 ttcgtcaacg gcagcgcggc gacggtggcg cagctgcgcg aggtcgccga gcacctggtc
    35761 gacatccacg gccagcacgc ctggcagagc ctcacgcggc cggccgcggt gcgcgcgctg
    35821 ctggacgagc aggccggcct ggacgccgct ccggtcgcgg cggcctacgc ggcc
//
LOCUS       DASVLN010000387         4240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_73563, whole genome shotgun sequence.
ACCESSION   DASVLN010000387 DASVLN010000000
VERSION     DASVLN010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73563"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VF453_09645"
     CDS             complement(<1..265)
                     /locus_tag="VF453_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEX7687959.1"
                     /translation="MNVKRFAARTSREALALVRQAFGVDAVVLSTKPCAEGIEVLAMA
                     PDSVAGLERLSASAEQVTADTPRKPQSQARAASAAPRSALAGAQ"
     gene            complement(262..2358)
                     /gene="flhA"
                     /locus_tag="VF453_09650"
     CDS             complement(262..2358)
                     /gene="flhA"
                     /locus_tag="VF453_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX7687960.1"
                     /translation="MDQLKKLLTQITGGNSALGKTLMAPVFIVMVLSMMVLPLPSFML
                     DLFFTFNIAMALMVMMVSANLVKPLDFAAFPTVLLVTTLLRLSLNVASTRVVLMEGHT
                     GPGAAGHVIESFGHFLIGGNFAVGLIVFAILVVINFVVVTKGAERIAEVGARFTLDAM
                     PGKQMAIDADLNAGLIDETMAKQRRREVADEAEFFGSMDGASKFVRGDAMAGILILFI
                     NVIGGFIIGVVQHGLSAGEAADSYILLAVGDALVAQIPGLLISVAAAMVVSRVGKDED
                     SGSQIRQQVFGSSKAMTITAGIVGTLGLIPGMPHVVFLLIAGGIGWFARFQKRQQAQE
                     AAAAAKKAEAPPPPKPADAEAGWDDVTQVDVLGLEVGYRLIALVDKSRSGDLLARIKG
                     VRKKFAQDVGFLPPSVHIRDNLELKPSMYRVTLRGAVIGEGEAFPGMHLAINPGGNAV
                     ALPGTKTFDPAFGLPAVWIEERQRESAQMAGYTVVDCPTVVATHLSHLMQVNAARLLG
                     RTETQALVEHVTKLAPKLVEDVIPKMIGIPTLQKTLQLLLEEGVHIRDFRSIVECLAE
                     FGATITDPAELARRIRTHIAPAIVQQIYGPVRELDVIALEPDLERMVTQALTSPHGAA
                     LDPGVADTISRQAADSARRQEDKGTPACLLVPDALRAPLARLLKRAAPRLKVLAHSEI
                     PETHSIRIGSIIGGAA"
     gene            complement(2376..3533)
                     /gene="flhB"
                     /locus_tag="VF453_09655"
     CDS             complement(2376..3533)
                     /gene="flhB"
                     /locus_tag="VF453_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEX7687961.1"
                     /translation="MADADRNLPASAKKIRKARADGNVPRSRDLGHFAAMGLAAAVLF
                     AAMPWLTAYVRQILADGLTFDASAVQSPQLAAEHVLGLFTRGLMIVVITGGLFAAAGV
                     VSNIALGGWNMSFKAVHPDLGRLNPIAGLGRLFNKQHVVGTLKAVALAIAVGVAGTLY
                     LKSHFEQLGGVLTAELPQGLATVGATLGGGFLAMTGVLAAWALIDVPWQRKQYLDRLK
                     MTKEEVKQEHKESEGNGEVKAKMKQKMRELARRRMMKAVPTADIVVMNPTHYAVALKY
                     DQDAMGAPRVVAKGTDRLALRIRELARDGGVPVLEAPPLARALYTHVEIDAEVPAMLF
                     AAVAQVLAWVYQLRQTPNKRAPDVHVPPELDPHDAGFVKPQRRRAPRATAE"
     gene            3775..4200
                     /gene="panD"
                     /locus_tag="VF453_09660"
     CDS             3775..4200
                     /gene="panD"
                     /locus_tag="VF453_09660"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEX7687962.1"
                     /translation="MFRTLLKSKIHRAVVTHCELHYEGSCAIDEDLLDAANLRENEQV
                     HIWNVDNGERLVTYALRAPRGSGIVSLNGSAARKAAAGDLVIIAAFALVAEAEVDQAE
                     PKLVFVDEKNRIKARPPFVGHTDESVAREAAQALAAARG"
BASE COUNT          631 a         1438 c         1555 g          616 t
ORIGIN      
        1 gctgggcgcc ggcgagcgcg ctgcgcggcg cggccgaagc ggcgcgggcc tgcgactgcg
       61 gcttgcgggg cgtgtccgcg gtgacctgct cggcgctggc cgacaggcgc tcgaggccgg
      121 caaccgagtc cggcgccatc gcgagcacct cgatgccctc cgcgcagggc ttggtggaca
      181 gcaccacggc gtcgacgccg aaggcctggc ggacgagggc gagcgcttct cgcgaagtcc
      241 gggcagcaaa gcgtttgacg ttcatgcggc gcctccgatg atcgagccga tgcggatgga
      301 atgggtctca gggatctcgc tgtgcgccag caccttcagg cgaggggcgg cgcgcttgag
      361 cagtcgggcc agcggcgcgc gcagcgcgtc gggcaccagc aggcacgcgg gcgtgccctt
      421 gtcctcttgc cggcgcgccg agtcggcggc ctgacggctg atcgtgtcgg ccacgcccgg
      481 atccagcgcc gcgccgtgcg gcgaggtgag ggcctgcgtg accatgcgct cgaggtcggg
      541 ctccagcgcg atgacgtcga gctcgcgcac ggggccgtag atctgctgca cgatcgccgg
      601 ggcgatgtgg gtgcggatgc ggcgcgccag ctcggcgggg tcggtgatcg tggcgccgaa
      661 ctcggccagg cactcgacga tggagcggaa gtcgcggatg tgcacgccct cttccagcaa
      721 cagctggagc gtcttctgca gggtggggat gccgatcatc ttgggaatga cgtcttcgac
      781 gagtttcggg gcgagcttgg tgacgtgttc gaccagggcc tgggtctcgg tgcgacccag
      841 gagacgagcc gcgttcactt gcatcaagtg tgaaagatgg gtcgccacga cggtcgggca
      901 atcaacgacc gtatagcctg ccatttgggc actttcgcgc tggcgctcct cgatccagac
      961 cgccggcagg ccgaacgcgg ggtcgaaggt cttcgtgccc ggcagcgcga cggcgttgcc
     1021 gcccgggttg atcgccaggt gcatgcccgg gaaggcctcg ccctcgccga tgacggcacc
     1081 gcgcagcgtg acgcggtaca tgctgggctt gagctcgagg ttgtcgcgga tgtgcaccga
     1141 cggcggcagg aagcccacgt cctgcgcgaa cttcttgcgc acgcccttga tgcgcgcgag
     1201 caggtcgccg gagcggctct tgtcgaccag cgcgatcagg cggtagccga cctccaggcc
     1261 gagcacgtcg acctgggtga cgtcgtccca gccggcctcc gcgtcggcgg gcttcggagg
     1321 cggcggcgcc tcggccttct tcgcggccgc ggcggcctcc tgggcctgct gccgcttctg
     1381 gaagcgcgcg aaccagccga tgccgccggc gatgagcagg aacacgacgt gcggcatgcc
     1441 ggggatcagg cccagcgtgc cgacgatgcc ggcggtgatg gtcatcgcct tggacgagcc
     1501 gaacacctgc tggcggatct gcgagcccga gtcctcgtcc ttgccgacgc gcgacacgac
     1561 catggcggcg gccaccgaga tcagcaggcc ggggatctgc gcgaccagcg cgtcgccgac
     1621 ggccagcagg atgtacgagt cggccgcctc gccggccgac agtccgtgct ggaccacgcc
     1681 gatgatgaaa ccgccgatga cgttgatgaa caggatcagg atgcccgcca tcgcgtcgcc
     1741 gcggacgaac ttcgaggcgc cgtccatgga gccgaagaac tccgcctcgt cggcgacctc
     1801 gcggcggcgc tgcttggcca tcgtctcgtc gatcaggccg gcgttcaggt cggcgtcgat
     1861 cgccatctgc ttgccgggca tcgcgtccag ggtgaagcgc gcgccgacct cggcgatgcg
     1921 ctcggcgccc ttggtgacga cgacgaagtt gatgaccacc aggatcgcga acacgatcag
     1981 gccgaccgcg aagttgccgc cgatcaggaa gtggccgaac gactcgatca cgtggccggc
     2041 ggccccgggg ccggtatggc cctccatcag cacgacgcgg gtggaggcga cgttgagcga
     2101 caggcgcagc agcgtcgtga ccagcagcac ggtcgggaag gccgcgaagt ccagcggctt
     2161 gaccaggttg gccgagacca tcatcaccat cagcgccatc gcgatgttga aggtgaagaa
     2221 caggtcgagc atgaacgacg gcagcggcag caccatcatg gacagcacca tgacgatgaa
     2281 gacgggcgcc atcagggtct tgccgagcgc cgagttgccg ccggtgatct gcgtgaggag
     2341 cttcttgagc tggtccatgg tcggtcagtg atggcttact cggccgtcgc gcgcggggcg
     2401 cggcggcgct gcggcttgac gaagccggcg tcgtgcgggt cgagctcggg cggcacgtgg
     2461 acgtccgggg cgcgcttgtt gggcgtctgg cgcagctggt agacccaggc gagcacctgc
     2521 gcgaccgcgg cgaacagcat cgcgggcacc tcggcgtcga tctcgacgtg ggtgtagagc
     2581 gcgcgggcga gcggcggcgc ctcgagcacc ggcacgccgc cgtcgcgggc gagttcgcgg
     2641 atgcgcaggg cgaggcgatc ggtgcccttg gcgacgacgc gcggcgcgcc catcgcgtcc
     2701 tggtcgtact tcagcgcgac cgcgtagtgc gtcgggttca tcacgacgat gtcggccgtg
     2761 ggcaccgcct tcatcatccg gcggcgggcg agctcgcgca tcttctgctt catcttcgcc
     2821 ttgacctcgc cgttgccctc gctctccttg tgctcctgct tgacctcttc cttggtcatc
     2881 ttcaggcggt cgaggtactg cttgcgctgc cacggcacgt cgatcagcgc ccaggcggcc
     2941 agcacgccgg tcatcgcgag gaagccgccg cccagcgtcg cgccgacggt ggccaggccc
     3001 tgcgggagct cggccgtcag cacgccgccg agctgctcga aatgcgactt caggtagagc
     3061 gtgcccgcga cgccgacggc gatcgccagc gcgaccgcct tgagcgtgcc gacgacgtgc
     3121 tgcttgttga acaggcggcc cagtcccgcg atcgggttga gccgcccgag gtcgggatgc
     3181 acggccttga agctcatgtt ccagccgccc agcgcgatgt tggagaccac gccggccgcc
     3241 gcgaacaggc cgccggtgat cacgacgatc atcaggccgc gggtgaacag ccccaggacg
     3301 tgctcggccg cgagctgcgg cgactgcacg gcgctcgcgt cgaaggtcag gccgtcggcc
     3361 aggatctggc gcacgtacgc ggtcagccac ggcatcgccg cgaacagcac cgccgccgcc
     3421 agccccatcg ccgcgaaatg gccgaggtcg cgcgagcgcg gcacgttgcc gtcggcgcgt
     3481 gccttgcgga tcttcttcgc ggaagctggc aggttgcgat cggcgtcggc catggcggga
     3541 tgcgggacgg agctcgggcg agattggagt cccgtcatgt tgccgggcgc ggccggaaac
     3601 ttgaggcgga aaaaggcgcc gaaacccccc ctgcttcgcg gcgcgacgtc gcgggcagcc
     3661 gacaggcacg tcccggcgcg gtcgtccagc cggagaaggc gggcaaaatg cggccttttc
     3721 ccgctccgtc cggcgcggcg gctccgtggc cgtcccgcca cgacgcccac gccgatgttc
     3781 cgcaccctcc tgaagtccaa gatccaccgc gccgtcgtca cgcattgcga gctccactac
     3841 gagggctcct gcgccatcga cgaggacctg ctggacgccg cgaacctgcg cgagaacgag
     3901 caggtgcaca tctggaacgt cgacaacggg gagcgcctcg tgacctacgc cctgcgcgcc
     3961 ccgcgcggca gcggcatcgt ctcgctcaac ggctcggcgg cgcgcaaggc ggccgcgggc
     4021 gacctggtca tcatcgcggc gttcgcgctc gtggccgagg ccgaggtcga ccaggccgag
     4081 ccgaagctgg tcttcgtcga cgagaagaac cgcatcaagg cacggccgcc gttcgtcggg
     4141 cacaccgacg agagcgtggc gcgggaggcg gcgcaggcgc tggcggccgc gcgcggctga
     4201 aggcgccccg gcggcgcctc gccggaaggc ccagcgaagc
//
LOCUS       DASVLN010000388         5296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_234270, whole genome shotgun sequence.
ACCESSION   DASVLN010000388 DASVLN010000000
VERSION     DASVLN010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234270"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF453_09665"
     CDS             complement(<1..221)
                     /locus_tag="VF453_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7687963.1"
                     /translation="MSAPLLLPSRFEDWCDPRRTGLLVYDMQVGVCRQVAQAAQVVER
                     CGAALEAARAAGMRVAFTRHQSCPRPWLGA"
     gene            complement(218..550)
                     /locus_tag="VF453_09670"
     CDS             complement(218..550)
                     /locus_tag="VF453_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX7687964.1"
                     /translation="MKVIAIDNFKPGVVLDDLRPYLRDEVANVWRLWKAGIVRENHAR
                     TDAGGVVIVFECSGVDEARRHVEDFPLTRAGLIEWQLVGCGAPLPLEFLFDPSVDVAE
                     PFDRTARH"
     gene            648..1007
                     /locus_tag="VF453_09675"
     CDS             648..1007
                     /locus_tag="VF453_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7687965.1"
                     /translation="MKRLARTFGCPTELALELLGGKWKTVILARLKDGPMRYGELRRR
                     IPRLSDKMLTQCLQGLQDRGLVARDPAAGATARYRLTPAGRSLRPLLQQLYDWGDAQA
                     DRLGVTILGRAEDTVDG"
     gene            complement(1011..1526)
                     /locus_tag="VF453_09680"
     CDS             complement(1011..1526)
                     /locus_tag="VF453_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687966.1"
                     /translation="MNAPANPVLDRDPLHRAYVRTESGHREVLERHAALSRPARTLLL
                     LIDASRQAQGWIDDIDGCAVQDLHALLQGGLVEPFVQKPLQADPQVDLAKALERWEFQ
                     PLYALLTSELRDRFGLFKGFRLVMKLEKCSGIGELRAFGLDFGERLRREHGEAAFRNL
                     CRRLVQPDDPS"
     gene            1733..2221
                     /locus_tag="VF453_09685"
     CDS             1733..2221
                     /locus_tag="VF453_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687967.1"
                     /translation="MTADATRGALRHVLIAALVAGTIDIAYAIAWSVAHGGTVRGLLQ
                     AVASGWLGKAAFTGGAASAALGLASHYGIVAAAAALYLAASRHSAWLRAHPWIAGPLY
                     GLAIFLTMDFVVVPLSAAPFHLHHRLWATASDLASHLFGVGLVIALVTRRALGPVTPV
                     PA"
     gene            complement(2202..2351)
                     /locus_tag="VF453_09690"
     CDS             complement(2202..2351)
                     /locus_tag="VF453_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HEX7687968.1"
                     /translation="MSRGIAWLLLLVSGVLDVAWAVSMKYAQGYTRPGWTIVAGIAVV
                     RLAPA"
     gene            complement(2348..2968)
                     /gene="gstA"
                     /locus_tag="VF453_09695"
     CDS             complement(2348..2968)
                     /gene="gstA"
                     /locus_tag="VF453_09695"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEX7687969.1"
                     /translation="MKLYYAAGTCSLSPHIVAREAGLALDLERVDIRPSPHLTASGVD
                     FTTVNPKGYVPALVLDDGAVLTEGAAIVQYLADLNPAAGLAPPAGTRARAELQSWLNF
                     IATELHKMYSPWLFHPEVGAQARQVALARIAERLAIVERHLAPGRPFLLGDTFTAADA
                     YLFTIVGWSEFADVDLSAFGHLRGFMTRVASRPAVREAMRAERAAT"
     gene            3123..4031
                     /locus_tag="VF453_09700"
     CDS             3123..4031
                     /locus_tag="VF453_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7687970.1"
                     /translation="MDDWNDLRLVLAIHRAGSLTAAATTLGVDHSTTFRRLKSLEERL
                     GVRLFERLPGGAYEATEAGARMAASAERMEDEALALARDLTGRDHRLHGRLRVTSSET
                     LAFSRLAPLLAAFREAHPGIVVELAIDNRVLDLSRREADIALRPVRPTEGDLWGRQLA
                     RVAWGLYAAPAYLEAHGRPANVRGLGRHAVIGWEDAASGIAAADWLARGAPAEAVVYR
                     SNSLVNHLVAAKAGIGLALLPCYLGDGDEGVVRALARPIAELEGELWIVTHADLRRTA
                     RIRAFFDVVGEQLAQQRALFEGRRRT"
     gene            4102..5292
                     /locus_tag="VF453_09705"
     CDS             4102..5292
                     /locus_tag="VF453_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7687971.1"
                     /translation="MSNHLRRMLAAAAFAAIAAASPARAQPPVDVQVVSATVQDSPIG
                     GAVVTLQRAGQSPLTGTSDGQGLVRFTGVADDANAQVVVSKPGYADLAMRCPCDAAVL
                     ALSPAMTDLDGLRIVLDWGKAPADLDAHLAFPGNHVYFLAKQGAGARLDVDSVRGRGP
                     ETITLAHRQPGVRYLYAVHDYSDKDSPATDRLSRSGARVFVYVGQTLVRTYAVPAGAG
                     NFWSVFAIGPDGEFQDLDVVKGVTSRDRLQTADFQDVIDHPAARQASLAPAAQTDARA
                     ANAQGEAAYHAGRVDEAIARYLQAIDLDGAYGQAYSNLGLAFQRAHRVAEAMWANRKA
                     LALAQGPGAATVRASSHYNNGRIYESARQWDDALREYRAAQAEAANPVYDHAIARMQQ
                     QGAR"
BASE COUNT          737 a         1914 c         1950 g          695 t
ORIGIN      
        1 gcgccgagcc agggccgcgg gcacgactgg tggcgcgtga acgcgacgcg catgccggct
       61 gcgcgcgcgg cctccagcgc ggcgccgcag cgctcgacga cctgcgccgc ctgcgccacc
      121 tggcggcaga cgccgacctg catgtcgtag accagcagcc cggtgcggcg cggatcgcac
      181 cagtcctcga agcgcgaggg caggagcagc ggggcgctca atgccgtgct gtccggtcga
      241 agggctcggc gacgtcgacc gacgggtcga acaggaactc gagcggcagc ggcgcgccgc
      301 agccgaccaa ctgccattcg atgaggccgg cgcgcgtgag gggaaagtcc tcgacatggc
      361 gccgcgcctc gtcgacgccg ctacattcga agacgatgac gacgccgccg gcgtcggtgc
      421 gggcatggtt ctcgcgcacg atgccggcct tccacaggcg ccagacgttc gcgacctcgt
      481 cgcgcaggta gggccggagg tcgtccagca cgacgccggg cttgaagttg tcgatggcga
      541 tgaccttcat gggggactca ggtggacacg gatgcacgca ggctacggat cgttccggcg
      601 agggtcaacg caccgcctgg tgcgtcgcgg ccgcggaggc tcgcgcgatg aagcgcctgg
      661 cgcgcacctt cggctgcccg acggagctcg cgctggagct gctgggcggc aagtggaaga
      721 ccgtgatcct ggcgcggctg aaggacggcc cgatgcgcta cggggagctg cggcgccgga
      781 tcccccgcct gagcgacaag atgctcaccc agtgcctgca gggcctgcag gatcgcggcc
      841 tggtggcgcg cgatcccgcg gcaggcgcga ccgcgcgcta ccgcctcacc ccggcggggc
      901 gctcgctgcg gccgctgctg cagcagctct acgactgggg cgatgcgcag gcggatcgcc
      961 tgggcgtgac gatcctcggt cgcgccgagg acacggtcga cggctgacgt tcaggacggg
     1021 tcgtccggct gcaccagccg ccggcacagg ttcctgaacg ccgcctcgcc gtgctcgcgc
     1081 cgcagccgct cgccgaagtc gaggccgaac gcccgcagct cgccgatgcc cgagcacttc
     1141 tccagcttca tcacgaggcg gaagcccttg aacaggccga agcgatcgcg cagctcggag
     1201 gtcagaagcg cgtacagcgg ctggaactcc cagcgctcca gggccttcgc gaggtcgacc
     1261 tgcgggtcgg cctggagcgg cttctgcacg aagggctcga ccagcccgcc ctgcagcagc
     1321 gcgtgaaggt cctgcacggc gcagccgtcg atgtcgtcga tccagccctg cgcctggcgc
     1381 gaggcgtcga tcagcagcag cagcgtgcgc gccggccgcg agagcgccgc atgccgctcg
     1441 agcacctcgc ggtgccccga ctcggtacgg acgtaagcgc ggtgcagggg atcgcggtcg
     1501 agaaccggat tcgcgggagc gttcatcgtg gaggcctcca tcgacctcga acgaggcggc
     1561 gcccatgtcg ccgggccgcc atcgcttgcg agcactctaa gcaattgcgg ccgcagcggg
     1621 aactgcgagc tttcgtagac ggcggcaatc gtgaaatccg accgctcggc cgcggcggcg
     1681 gtatcgtgcg cgggggcgtc gcgccgcggc ggcctgcccg acaggagagt ccatgactgc
     1741 cgatgcgacc cgaggcgcgc tgcgccacgt cctgatcgcc gcgctcgtcg ccggcacgat
     1801 cgacatcgcc tacgcgatcg cgtggagcgt cgcgcacggg ggcaccgtgc gcggactgct
     1861 gcaggccgtg gccagcgggt ggctgggcaa ggcggccttc acgggcggcg ccgcgagcgc
     1921 cgcgctcggg ctcgcgtcgc actacggcat cgtggcggcc gccgcggcgc tgtacctggc
     1981 cgcgagccgt cacagcgcgt ggctgcgcgc gcatccgtgg atcgcggggc cgctctacgg
     2041 cctggccatc ttcctgacga tggacttcgt cgtcgtgccg ctgtcggcgg cgcccttcca
     2101 cctgcaccac cggctctggg cgacggcgag cgacctggcg tcgcacctgt tcggcgtggg
     2161 cctcgtcatc gcgctcgtga cgcggcgcgc cctcgggccc gtcacgccgg tgccagcctg
     2221 accaccgcga tgccggccac gatcgtccac ccgggcctcg tgtacccctg ggcgtatttc
     2281 atcgacaccg cccaggccac gtccagcacg cccgagacga gcagcagcag ccatgcgatc
     2341 ccgcggctca cgtcgccgcc cgctccgccc gcatcgcctc gcgcacggca ggccggctcg
     2401 cgacgcgggt catgaagccg cgcaggtgcc cgaaggccga gaggtcgacg tccgcgaact
     2461 ccgaccagcc cacgatcgtg aacaggtagg cgtcggcggc ggtgaacgtg tcgcccagca
     2521 ggaagggccg gcccggggcg agatggcgct cgacgatggc caggcgttcg gcgatccggg
     2581 cgagagcgac ctgccgggcc tgcgcgccga cctccggatg gaacagccac gggctgtaca
     2641 tcttgtgcag ctcggtggcg atgaagttga gccaggactg gagctcggcg cgcgcgcgcg
     2701 tgccggcggg cggcgcgagg ccggcggcgg gattcaggtc ggccaggtac tgcacgatgg
     2761 ccgcgccttc cgtcagcacg gcgccgtcgt cgaggaccag ggcgggcacg tagcccttgg
     2821 gattgacggt ggtgaagtcg acgcccgacg cggtcaggtg gggcgaggga cggatgtcga
     2881 cgcgctcgag gtcgagcgcg aggcccgctt cgcgcgcgac gatgtggggc gagagcgagc
     2941 aggttccggc ggcgtagtag agcttcatgg cggctgttcc ggttgcgaag ggttggcgtc
     3001 gccgccaggc tggaccggcg gcgatcggga tcaatgtagt cctggcgttc ttgcaagtca
     3061 cgatgcgatt tcgcaatcga cgcttgcatc tacgcaagcc gtgcgctgct atgctcgcgc
     3121 gcatggacga ctggaacgac ctccgcctgg tactcgcgat ccaccgcgcc ggcagcctga
     3181 cggccgcggc gaccacgctg ggcgtcgacc actcgacgac cttccggcgc ctgaagtcgc
     3241 tggaggagcg cctcggcgtg cgcctgttcg agcgcctgcc cggcggcgcc tacgaggcga
     3301 ccgaggccgg tgcgcgcatg gccgccagcg ccgagcgcat ggaggacgag gcgctggcgc
     3361 ttgcgcgcga cctcaccgga cgggaccacc gactgcacgg ccgcctgcgg gtgacctcgt
     3421 ccgagacgct cgcgttcagc cgcctcgcgc cgctgcttgc cgcgttccgg gaggcgcatc
     3481 cgggcatcgt cgtggagctg gcgatcgaca accgcgtcct cgacctctcg cgccgcgagg
     3541 ccgacatcgc gctgcgaccc gtgcggccga cggagggcga cctgtggggc cgccagctcg
     3601 cgcgcgtggc ctggggcttg tacgcggcgc ccgcctatct cgaggcccac ggccggccgg
     3661 ccaacgtgcg cggcctcggt cgccacgcgg tgatcggctg ggaggacgcg gcgtcgggca
     3721 tcgccgccgc cgactggctc gcccgtggcg ccccggccga ggccgtcgtc taccgctcga
     3781 acagcctcgt caaccacctc gtcgccgcga aggccggcat cgggctggcg ctgctgccgt
     3841 gctacctggg cgacggcgac gagggcgtgg tgcgcgcgct ggcgcggccg atcgccgaac
     3901 tcgagggcga gctgtggatc gtcacgcacg ccgacctgcg caggacggcg cgcatccggg
     3961 ccttcttcga cgtcgtcggc gagcagctcg cgcagcagcg ggcgctgttc gaagggcggc
     4021 ggcggacata gcctgcgtcg ctacactgcg tgcgccatgc ggccggcgcg cgccggcgct
     4081 gttcacgatg aaagggagcc catgtcgaac cacttgcggc gcatgctcgc ggcggccgcg
     4141 ttcgctgcga tcgccgcggc gtcgcccgcc cgtgcgcagc cgcccgtcga tgtccaggtc
     4201 gtcagcgcca ccgtccagga cagcccgatc ggcggcgccg tcgtcacctt gcagcgggcc
     4261 gggcagtcgc cgctcacggg cacgtcggac ggccaggggc tggtgcgctt caccggcgtc
     4321 gcggacgacg cgaacgcgca ggtggtggtg agcaagccgg gctacgccga cctcgcgatg
     4381 cgctgtccct gcgacgccgc ggtgctcgcg ctcagccccg cgatgaccga tctcgacggc
     4441 ctgcgcatcg tgctggactg gggcaaggcg ccggccgacc tcgacgccca tctcgccttc
     4501 ccgggcaacc acgtgtactt cctcgccaag cagggcgcgg gcgcgcggct ggacgtcgac
     4561 agtgtccgcg gtcgcggacc cgagacgatc acgctcgccc atcgccagcc cggcgtgcgc
     4621 tacctctatg ccgtgcacga ctactcggac aaggacagcc ccgcgaccga ccgcctctcg
     4681 cgcagcggcg cgcgcgtgtt cgtctacgtc ggccagacgc tggtgcgcac gtacgccgtg
     4741 ccggcgggcg cgggcaactt ctggtcggtg ttcgcgatcg ggcccgacgg cgaattccag
     4801 gacctcgacg tcgtcaaggg cgtgaccagc cgcgaccgcc tgcagaccgc cgatttccag
     4861 gacgtgatcg accacccggc ggcgcgccag gcgagcctcg cgccggcggc gcagaccgac
     4921 gcccgcgccg ccaacgcgca gggcgaggcg gcctaccacg ccggccgcgt cgacgaggcg
     4981 atcgcccgct acctgcaggc catcgacctc gacggcgcat acggccaggc gtattccaac
     5041 ctcggcctgg cgttccagag ggcgcaccgc gtcgccgagg cgatgtgggc caaccgcaag
     5101 gccctcgcgc tcgcgcaggg gccgggcgcc gcgacggtgc gcgcgagctc gcactacaac
     5161 aacggccgca tctacgagtc ggcgcgccag tgggacgacg ccctgcgcga gtaccgtgcc
     5221 gcgcaggccg aagccgcgaa cccggtctac gaccacgcga tcgcgcgcat gcagcagcag
     5281 ggggcgcgat gagggc
//
LOCUS       DASVLN010000389         4791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_181641, whole genome shotgun sequence.
ACCESSION   DASVLN010000389 DASVLN010000000
VERSION     DASVLN010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181641"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VF453_09710"
     CDS             <1..613
                     /locus_tag="VF453_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7687972.1"
                     /translation="SVRAHGRRACTLQADVAVEAEVEAMFRRLDDELGPLSGLVNNAG
                     IIDRIARVDEMAAGRVERMLRVNVVGPFACAREAVRRMSTRHGGAGGAIVNVSSAAAR
                     LGSPGVFVDYAASKAAIDALTLGLAREVAGEGVRVNGVRPGMIDTEIHAASGMANRAQ
                     EAVPTIPMRRLGSAGEVADAIVFLLSDAATYVTGATLDVSGGR"
     gene            907..2208
                     /locus_tag="VF453_09715"
     CDS             907..2208
                     /locus_tag="VF453_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7687973.1"
                     /translation="MKLIRSAAAVLAAALLGTMAPAALAGVLAISFGADDAAQREAYK
                     TLVNDFRSANPDVEVHLTLTDTPSYRKALPGTLDTDGAPDVFNWFAGDQMKSMALRGE
                     LDDVSDLWKANTWWNTFPAAAATVAGRQYALPYQYYPWGLFFRRDVLERAGVHEPPRD
                     LSAMLAACSKLRRTGFTPIALGGRTGPAIAAWFDFIDMRANGYEFHQQLLDGKVPYND
                     GNVRRAFSMWRQLIDAKCFDPNATAVDERGAAALLYAGRAGMMLTGTVVSASFPEIVR
                     PQIDYEKFPQIDASQPPAEAAPTDTFHIAAHARNKADARRFLKFAGSNSADGRLARAM
                     GSFPTNKYSPVAGSVLDLSSYKVLTDAKTNLVQGYDRDVPADMAAQGVKAFQEFLAKP
                     DNMYALLVRLDGVRETAYANAAALEAAAAAPPPHGRHASTN"
     gene            2372..2752
                     /locus_tag="VF453_09720"
     CDS             2372..2752
                     /locus_tag="VF453_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7687974.1"
                     /translation="MTQDAFCWVSIPATDIDRAQRFYETLLACSLQRQQNGPQTIALF
                     PSGEGRVGGGLFHGPGVPAPSTDGPIAYVQARPSIDVVLARAGELGATVLMPKFELPG
                     GAGIIAQIVDSEGNRLGLWAPAAA"
     gene            2768..3061
                     /locus_tag="VF453_09725"
     CDS             2768..3061
                     /locus_tag="VF453_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687975.1"
                     /translation="MPSHTSERLAALVPVVEFRERPARPASVAAYEAWRVDATRPAVP
                     VRRGRGGLAAGLGLGVAIGLAIGLGLGVAVGVGLGIGVGIGLDVVGRLCDELF"
     gene            3090..3824
                     /locus_tag="VF453_09730"
     CDS             3090..3824
                     /locus_tag="VF453_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX7687976.1"
                     /translation="MRRADRLFQIVQLLRGRRLSTAAWLADKLEVSLRTVYRDVADLQ
                     AQGVPIDGEAGVGYRLSAGFDLPPLMFTSTEAQALVAAVRLVQARLDRGLAEAAEDAL
                     GKIVAVLPPLARAAAETLPLYSGRRSFDEVTAGTLATLRDAAVARRFVHFDYADARGA
                     ATSRTVRPLACVDWEAAWTLVAWCEHRSAFRSFRLDRMRRLKALDDRFRDEPGRTLAD
                     FLREVRGRTVPRLPVVDDALTDPPGL"
     gene            3946..4317
                     /locus_tag="VF453_09735"
     CDS             3946..4317
                     /locus_tag="VF453_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687977.1"
                     /translation="MARYTKELSAALALYAALLVGSIELLQHVAVPAPWRDAIALSPM
                     AAAAVMAWVMLRQLRRMDELQVRIQLEALGFAFAGTALATFSYGFLEGIGWPRLSMFA
                     VWPLMAGLWIVGQLLARRRYQ"
     gene            4314..4508
                     /locus_tag="VF453_09740"
     CDS             4314..4508
                     /locus_tag="VF453_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7687978.1"
                     /translation="MKNRIRELRTARGWSQTDLGERLGVSRQTVNAIENERYDPSLPL
                     AFVIARVLELPIEQLFFEEN"
     gene            4510..>4791
                     /locus_tag="VF453_09745"
     CDS             4510..>4791
                     /locus_tag="VF453_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687979.1"
                     /translation="MPLSTTPRPRRAAAWADRCAARAAESTSSSKENAMSCIRDFLAH
                     PSKACRSDRSRRVAVLVALLVSLGVWPFQRAHAAAAAQAIAVQVTGSGPD"
BASE COUNT          648 a         1823 c         1657 g          663 t
ORIGIN      
        1 ctcggtgcgc gcgcacggac ggcgcgcgtg cacgctgcag gccgacgtcg ccgtcgaggc
       61 ggaagtcgag gcgatgttcc gccgcctcga cgacgaactc ggcccgctgt cgggcctcgt
      121 caacaacgcc ggcatcatcg atcgcatcgc gcgcgtcgac gagatggcgg ccggccgcgt
      181 cgagcgcatg ctgcgggtca acgtcgtcgg ccccttcgcc tgcgcgcgcg aggccgtgcg
      241 gcgcatgagc acgcgccacg gcggcgcggg cggggcgatc gtcaacgtgt cgtcggccgc
      301 ggcgcggctg ggatcgccgg gcgtcttcgt cgactacgcg gcaagcaagg cggcgatcga
      361 cgcgctgacg ctgggactcg cccgcgaggt cgccggcgaa ggcgtgcgcg tcaacggcgt
      421 gcggccgggg atgatagaca ccgagatcca cgccgccagc ggcatggcca accgcgcgca
      481 ggaggcggtg ccgacgatcc cgatgcgccg gctgggcagc gccggcgagg tcgccgacgc
      541 gatcgtcttc ctgctgtcgg acgccgcgac ctacgtcact ggcgccacgc tcgacgtcag
      601 cggcggccgc tgagcgaccg acggttcgac caggaaatcg ggccagggcc gccgaaatgg
      661 cgacaaaccc tcgcttttgc cgatcagagg cgccgcaatc ggtggatttc ccgacgtttc
      721 gacgcccggg cattgtcacg aagccctcac aatcgatgcc cgcaacgacg ccagtcggcg
      781 gcccgcaggc gggagaaccg tgacatcgtg cccgcttcgc gtggcaggag aaggggtcag
      841 cccctagccg gtcgcatggg ggccgaccac cataatcttc cggtcggtgg gtagagggac
      901 acgaacatga aactgattcg cagcgccgcg gcggtcctgg ccgcggccct cctgggcacc
      961 atggcgcccg ccgcgctggc cggcgtgctg gccatcagct tcggcgccga cgacgccgcc
     1021 cagcgcgagg cctacaagac gctggtcaac gacttccgct ccgccaaccc ggacgtcgag
     1081 gtccatctca cgctcaccga cacccccagc taccgcaagg cgttgcccgg gacgctcgac
     1141 accgacggcg cgcccgacgt gttcaactgg ttcgccggcg accagatgaa gagcatggcg
     1201 ctgcgcggcg aactcgacga cgtgagcgac ctctggaagg ccaacacgtg gtggaacacc
     1261 tttcccgccg cggccgcgac cgtcgccggc cgccagtacg cgctgcccta ccagtactac
     1321 ccgtggggcc tgttcttccg tcgcgacgtc ctcgagcgcg ccggcgtgca tgagccgccg
     1381 cgcgacctga gcgccatgct cgccgcctgc tccaagctgc gccgcaccgg cttcacgccg
     1441 atcgcgctgg gcgggcgcac cggccccgcg atcgccgcgt ggttcgactt catcgacatg
     1501 cgcgccaacg gctacgagtt ccatcagcag ctgctcgacg gcaaggtccc gtacaacgac
     1561 ggcaacgtgc gccgcgcgtt ctcgatgtgg cgccagctga tcgacgccaa gtgcttcgac
     1621 ccgaacgcga ccgccgtcga cgagcgcggc gccgcggcgc tgctgtacgc cggccgcgcc
     1681 ggcatgatgc tgaccggcac ggtcgtgagc gcgagcttcc cggagatcgt gcggccgcag
     1741 atcgactacg agaagttccc gcagatcgac gcctcccagc cgcccgccga ggccgcgccg
     1801 accgacacct tccacatcgc cgcccatgcc cgcaacaagg ccgacgcccg gcgcttcctg
     1861 aagttcgcgg gcagcaattc ggcggacggc cggctcgcga gggcgatggg ctcgttcccg
     1921 accaacaagt actcgcccgt ggccggcagc gtcctggacc tcagctcgta caaggtgctg
     1981 accgacgcca agaccaacct cgtgcagggc tacgaccgcg acgtccccgc cgacatggcc
     2041 gcccagggcg tcaaggcctt ccaggaattc ctggccaagc cggacaacat gtacgcgctg
     2101 ctcgtgcgcc tggacggcgt gcgcgagacc gcctacgcca atgccgccgc cctcgaggcc
     2161 gcggccgccg cgccgccgcc gcacggccgc cacgcgagca cgaactgaca cgcccggacg
     2221 gccaccggcc gacggtcggg cgtcgtccgg tcgccgatcc gcgccgcgca tcgtcgcggc
     2281 tgccgccacg ctgctgacaa cacgctggca gcagccccgg cgcagcatgg agacctcttc
     2341 cacatcgacg cgagtcgacg aaaggtctcc catgacgcaa gacgccttct gctgggtctc
     2401 gatccctgcc accgacatcg accgcgccca gcggttctac gagacgctgc tcgcgtgctc
     2461 cctgcagcgg cagcagaacg ggccgcagac gatcgccctg ttcccgagcg gcgagggccg
     2521 cgtcggcggc ggcctgttcc acggcccggg cgtgcccgca cccagcaccg acggcccgat
     2581 cgcgtacgtc caggcgcggc cgtccatcga cgtcgtgctc gcccgcgccg gcgagctggg
     2641 cgcgaccgtg ctgatgccca agttcgagct gcccggcggc gccggcatca tcgcgcagat
     2701 cgtcgacagc gagggcaacc gcctcggcct gtgggcgccg gccgcggcct gatccatgga
     2761 gcacgcgatg ccctcgcaca cctccgagcg gctcgccgcg ctcgtgccgg tcgtcgagtt
     2821 ccgcgagcgg ccggcgcggc cggcctccgt cgccgcctac gaggcctggc gcgtcgacgc
     2881 gacgcgtccc gccgtgcccg tgcgccgcgg ccgcggcggc ctggccgcgg gcctcgggct
     2941 gggcgtcgcg atcgggctgg ccatcggcct cgggctgggc gtggcggtcg gcgtcgggct
     3001 gggcatcggg gtcggcatcg gcctggacgt ggtcggacgg ctctgcgacg aattgttctg
     3061 aggcggtgcg ctctgctcca atggcaggga tgcgccgcgc cgaccgcctg ttccagatcg
     3121 tccagctcct gcggggccgc cggctgtcga cggccgcctg gctcgcggac aagctcgagg
     3181 tctcgctgcg caccgtctac cgcgacgtcg ccgacctgca ggcgcagggc gtgccgatcg
     3241 acggcgaggc gggcgtcggc taccggctgt ccgccggctt cgacctgccg ccgctgatgt
     3301 tcacgtcgac ggaggcgcag gccctcgtcg ccgccgtgcg gctggtgcag gcgcggctgg
     3361 atcgcgggct cgccgaggcg gccgaggacg cgctgggcaa gatcgtcgcc gtgctgccgc
     3421 cgctcgcgcg cgccgcggcc gagacgctgc cgctgtactc cggccgccgc tcgttcgacg
     3481 aggtgaccgc cgggaccctg gccacgctgc gcgacgcggc ggtcgcgcgc cgcttcgtgc
     3541 acttcgacta tgccgacgcc cgcggcgcgg ccacctcgcg cacggtgcgc ccgcttgcct
     3601 gcgtggactg ggaggcggcc tggacgctgg tcgcctggtg cgagcaccgc agcgccttcc
     3661 gcagcttccg cctcgaccgc atgcgccggc tcaaggcgct cgacgatcgc ttccgcgacg
     3721 agcccggccg caccctcgcg gacttcctgc gcgaggtgcg cgggcgcacc gtcccgcgac
     3781 tgcccgtcgt agacgacgcc ctgaccgacc cgcccggcct ctgagccccg gccatcccga
     3841 cctgtaaagg gggctttaca gctccggtgc gtgcgcgcac aatggaaagc atcctttcca
     3901 cacgccccga ttggagccgc gatgcccgtt tcgaagtcgt ccccgatggc ccggtacacg
     3961 aaggagttgt cggcggcgct ggcactctac gccgcgctgc tggtcggctc gatcgagctg
     4021 ctgcagcacg tcgccgtgcc cgcgccctgg cgcgacgcga tcgcgttgag cccgatggcc
     4081 gccgcggccg tcatggcctg ggtgatgctg cgccagctgc gccggatgga cgagctgcag
     4141 gtgcgcatcc agctcgaggc gctgggcttc gccttcgccg gcaccgcgct cgcgacgttc
     4201 agctacggct tcctcgaggg catcgggtgg ccgcggctgt cgatgttcgc cgtctggccg
     4261 cttatggccg ggctctggat cgtcggccag ctgctcgccc ggaggcgcta ccaatgaaga
     4321 accggatccg cgagctgcgc accgcccgcg gctggtcgca gaccgatctc ggcgagcgcc
     4381 tcggcgtctc gcggcagacg gtcaacgcga tcgagaacga gcgctacgac ccgagcctcc
     4441 cgctcgcctt cgtgatcgcg cgcgtcctcg agctgccgat cgaacagctc ttcttcgagg
     4501 agaactgaca tgcccctttc caccacgccg cggccgcggc gagccgccgc atgggccgat
     4561 cgttgcgcgg cccgcgccgc cgagtccact tcctcgtcca aggagaacgc catgtcctgc
     4621 atccgcgact tcctcgccca tccctcgaag gcctgccgct ccgaccggag ccggcgcgtc
     4681 gccgtcctcg tcgccctgct cgtgtcgctc ggcgtctggc cgttccagcg cgcgcacgcg
     4741 gccgccgccg cgcaggcgat cgcggtgcag gtgacgggca gcgggccgga c
//
LOCUS       DASVLN010000390         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_234394, whole genome shotgun sequence.
ACCESSION   DASVLN010000390 DASVLN010000000
VERSION     DASVLN010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234394"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1485
                     /locus_tag="VF453_09750"
     CDS             79..1485
                     /locus_tag="VF453_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687980.1"
                     /translation="MAQQDNRFGGSDDWQHGSQQSQASGSQQGGQQGDWRDPGASRRG
                     HEQYEHGGGQYGDPGRSSARGRDWNPGGGQGSGGYQGAGGDDYGNRGITQGGDYSSAQ
                     GGRSPGQGGREQGAFGQGMSGQGYGQGGRGYGQGYPSSNYGRAFEDQREFGRYGGGYP
                     GGEGGGYGGGYQGGQPDHRGAYRGGDAGQDRGYGGGYGGGHPGGYRGGYEPGRESQGR
                     GQAWGGGYGTGDYGSQGMGGAGGYGSSSFGPESNWGSGSYGGGDFSQRGGYGGAHDQD
                     RGQGGYSGWRESSGGGAGGWRGGQGSGMGMGMGHEDHDPDYHQWRGEQLRRLDQDYAD
                     WRQHRYQRFSSDFDEWRRNRDRSAGGAGQQGAGQSSLGAQSGQASHSGMGAQSGQGLH
                     SSSGSGSQSGHSGQAGQSGITSAAGSSGGTGIESSASSASQQHLGSSGTGAAAKGGGA
                     GESSTGHQPAGSTSTTRK"
     gene            complement(1664..2431)
                     /locus_tag="VF453_09755"
     CDS             complement(1664..2431)
                     /locus_tag="VF453_09755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7687981.1"
                     /translation="MPRFEGRTVIVTGAGSGIGQGIALRFSREGANVVLAGRTKGRLD
                     ETAAQMAAPPRTLVQVCDVRELAQVEALVRAAVERFGGLDVMVNNAGIGPDGTATETS
                     LEDWHDVLQTDLTGVFHGCRAALPELEKRRGCIVNTASVSGLGADWALAAYNAAKGGV
                     VNLTRALALDYGRRGVRINAVCPSLTRSNMTREMLEDKALMAKFMERLPLGRPAEPAD
                     IAAVVAFLASEDAGFVNGVNLPVDGGLSASNGQPPMG"
BASE COUNT          358 a          926 c         1060 g          321 t
ORIGIN      
        1 tctgcacgaa gcgcttcgcg gcccggccgc gcggccttcg tgccaaaccg acttcacgcg
       61 gaaaggatgg ctacgcacat ggctcagcaa gacaatcgct tcggcggctc ggacgactgg
      121 cagcacggct cgcagcagtc gcaggcctcg ggatcccagc aaggcggcca gcagggcgac
      181 tggcgcgatc cgggggcgtc gcgacgcggg cacgagcaat acgaacacgg cggtggccag
      241 tacggcgacc ccgggcgctc gagcgcgcgc ggccgcgact ggaacccggg cggcggccag
      301 ggctcgggcg gctaccaggg cgccggcggc gacgactacg gcaaccgcgg catcacgcag
      361 ggcggcgact actcttcggc gcaaggcggc cgctcccccg gccagggcgg ccgcgagcag
      421 ggcgccttcg ggcagggcat gtccggacag ggctacgggc agggcggccg agggtacggc
      481 caggggtacc cgtcgtcgaa ctacgggcgc gcgttcgagg accagcgcga gttcggccgc
      541 tacggtgggg gctatcccgg cggcgagggc ggcggatacg gtggcgggta ccagggcggg
      601 cagccggacc atcgcggcgc ctaccggggt ggagatgccg gccaggatcg cggatacggc
      661 ggcgggtatg gaggcggcca tccgggcggc taccgcggcg gctacgagcc gggccgcgag
      721 tcgcagggcc gcggccaggc ctggggcggc ggctatggta ccggcgacta cggttcgcag
      781 ggcatgggcg gcgcgggcgg ctacggatcg agcagcttcg ggccggaaag caactggggc
      841 tcgggcagct acggcggcgg cgacttctcg cagcgcggcg gctacggcgg cgcccatgac
      901 caggaccgtg gccagggcgg atactcgggc tggcgcgagt cctcgggagg aggtgccgga
      961 ggctggcgcg gcggccaggg ctcgggaatg ggaatgggca tgggccacga ggaccacgac
     1021 cccgactacc accagtggcg cggcgagcag ctgcgccgcc tggatcagga ctacgccgac
     1081 tggcgccagc atcgctacca gcgcttttcc agcgacttcg acgaatggcg ccgcaaccgc
     1141 gaccggagcg cgggcggtgc cggccagcag ggagccggcc agtcgagcct gggcgcgcag
     1201 tccggtcagg cttcgcattc ggggatgggc gcgcagtccg gccagggcct gcactcgagc
     1261 tcgggctcgg gctcgcagtc cgggcactcg ggccaggccg gccagtcggg catcacgtcg
     1321 gcggccggct cgtcgggcgg cactgggatc gagtcctcgg cctcgtcggc gtcgcagcag
     1381 cacctgggct cgtcgggcac cggcgcggcg gccaagggcg gcggcgcggg cgagtcctcg
     1441 acgggccatc agcccgccgg ctcgacgtcg acgacccgga agtgatggcg cgctgatgcc
     1501 gcccggccgg ccggcgcgtg cgccggccgg ccgctcggtt tcctttgcgc gatcgcggga
     1561 ggggacgtgg ggtcgcccga ggccctgaac gccgccgggg cgcggatcgg cggtcgatca
     1621 tgcgatacgg atgccggcat ccgtatcgcg gcgccgcgca cgctcagccc atcggcggct
     1681 gcccgttgga agcgctcagg cccccgtcca cgggcaggtt cacgccgttg acgaagcccg
     1741 cgtcctcgct cgccaggaag gcgacgacgg cggcgatgtc ggccggctcg gcgggacgac
     1801 cgagcggcag gcgctccatg aacttcgcca tcagcgcctt gtcctcgagc atctcccggg
     1861 tcatgttcga gcgggtgagc gacgggcaga ccgcgttgat ccgcacgccg cgccggccgt
     1921 agtcgagcgc gagggcgcgc gtgaggttga ccacgccgcc cttggccgcg ttgtaggcag
     1981 ccagcgccca gtcggcgccg aggcccgaca cggaggcggt gttgacgatg cagccgcggc
     2041 gcttctcgag ctcgggcagc gccgcgcggc agccgtggaa cacgccggtc aggtcggtct
     2101 gcaggacgtc gtgccagtcc tcgagcgagg tctcggtggc cgtgccgtcg ggtccgatgc
     2161 cggcattgtt gaccatcacg tcgaggccgc cgaagcgctc gacggccgca cgcacgagcg
     2221 cctccacctg cgccagctcg cgcacgtcgc agacctgcac cagcgtgcgc ggcggcgcgg
     2281 ccatctgggc ggcggtctcg tccaggcggc ccttggtgcg gcccgccagc acgacgttgg
     2341 cgccctcgcg cgagaagcgc agcgcgatgc cctggccgat gccggagccg gcgccggtga
     2401 cgatgacggt tcttccttcg aagcgtggca tggcgatctc ctcgtgtcgc gaccgggggt
     2461 gcagggccag ccgcgtgcct gcgcggccgc ccggcgcccg ccgggtgctg gcgcgaatgg
     2521 cgttcaagtc gagcgcagcg cggccgaaag agcgttcatg agcgaaccgt cctccgccgc
     2581 cgacatgccc gcgctggccg cccgcctgct cgcgcgttgg gaccgggccg ggctggcggt
     2641 gcgctgcggc tggcgcacgc ggtca
//
LOCUS       DASVLN010000391         4274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_127992, whole genome shotgun sequence.
ACCESSION   DASVLN010000391 DASVLN010000000
VERSION     DASVLN010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127992"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VF453_09760"
     CDS             <1..665
                     /locus_tag="VF453_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEX7687982.1"
                     /translation="ADVPAWSCEEVVGRHVDQVVRRHAPRLIGLQEVQRLLRTVQADA
                     PELAAEVSRAVPAQRIAEVLRRLLQEGIPVRNLHAIFESLALRGPAESDVIALTELLR
                     IDLGRYITSRHAGSARRIEAILFESSLLDRVLAAIERSARGNLLLLSPAVAQDVRDQV
                     RTILGGTGGAVVAIVSSDARRYVKTLLEPIAPELPVLSYQELDADVALHPVGWVTNPQ
                     AA"
     gene            699..965
                     /locus_tag="VF453_09765"
     CDS             699..965
                     /locus_tag="VF453_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HEX7687983.1"
                     /translation="MTPQNVLDITREGLQLVFWVSLPVVLVAAITALVVALGQAVTQI
                     QDQSIGQSIRLISVMLTLVVLAGWLGRTVLLFAERAFDSLVRLA"
     gene            962..2833
                     /gene="sctC"
                     /locus_tag="VF453_09770"
     CDS             962..2833
                     /gene="sctC"
                     /locus_tag="VF453_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HEX7687984.1"
                     /translation="MKRLFGFLAGGALATALLCAAPAAGAAELPWAGKPYQIVANEKP
                     LADLLRELAASQGTTAVIDPKVSGTISGRFSGSPLKTLNSLCATYGLSWYYDGSFLYV
                     DPSADARSEVLSIGNATGNASWLIQSLTQLRIYDSRYPLLVSGRDGTARVTGPKRYVE
                     MVRQVARLLDSKSALNDGAEIRLFPLKYAWAGDFKITRSGKEVAVPGVANTLRSLYSH
                     ANGGSSASGTAGAVARQYAAYQAGADRQLRLSTGDLVNAPMVQLPGLAPPMTGGDAGD
                     AGSAPAAGLSAGDGGLPQFQADTRLNAVLVRDLPTHMAQYEKLIAAMDQRPRLVEIEV
                     TIMDVSTDTLDSLGVDWRAHSSHVDFQTGNATNSPLTLGGATSESGQTGGLCCTNANG
                     LPTTPLGSIFTAAIGNSLRDYLLARVSALAQTGNADVVARPKVLTLDNNEAVLENLSD
                     FYVSVAGFQDASLFKVTTGTSVRVTPMIVDENGGRGVMMTIDIQDGNIDPTQSVGQIP
                     VVRHRTVDTQALIDEGSSLLLAGYTSESKSTAASGVPVLSSIPIVGNLFKYNQKQGSK
                     EERLYLLTPRLVTPGLAANGAALAPLPTQDRPSLPVQDAPVPPAAVPVSSAHAGRRT"
     gene            2830..3939
                     /locus_tag="VF453_09775"
     CDS             2830..3939
                     /locus_tag="VF453_09775"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7687985.1"
                     /translation="MKATPEFVTPGWCLRFLSGAMKGRTIELRPGANVLGSAGECDVM
                     LPGGDVLPRHLVFNVGELVVSVQRTGEGEVLLNREPMQQPRRSLVPGDVVTVGRIELQ
                     LERSYPAVDRADPMFAGPESVLPGDTPVHVPPSGRGRGFWAGAALLVLSCAGLLGVGL
                     WAGGEDRPAAAADPAAVARVIAPFTELEAVAAPGGQVVVKGFVESRLRRQQLEQALAP
                     MGRRVTVEAVPVDELVEQARRYLGDPALGVSYAGKGQLVVTGATDEGASRDRLRRLAE
                     DLHPSVLVTDRVSWRHAPAPDRDAALRAHWAAWQDALPSRLVGITEDEDGLRSIQLAD
                     GTRYYEGAPLKSGAQIAHIGADGLVLEGGARDGAK"
     gene            4057..4260
                     /locus_tag="VF453_09780"
     CDS             4057..4260
                     /locus_tag="VF453_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687986.1"
                     /translation="MSTVDTSIAAMQQAAADSARLQAASISVQTQIQGATTSAQTVNS
                     ASAAATETAKGVASDTKAAARAS"
BASE COUNT          584 a         1569 c         1549 g          572 t
ORIGIN      
        1 ccgcggacgt gcccgcctgg agctgcgagg aggtcgtcgg ccgccacgtc gaccaggtcg
       61 tgcgccgcca cgcgccgcgg ctgatcggcc tgcaggaggt gcagcgcctg ctgcgcacgg
      121 tgcaggcgga cgcgcccgag ctcgcggccg aggtctcgcg cgccgtgccc gcgcagcgca
      181 tcgccgaggt gctgcggcgc ctgctgcagg aaggcatccc ggtgcgcaac ctgcacgcca
      241 tcttcgagag cctggccctg cgcggcccgg cggagtccga cgtcatcgcg ctgaccgagc
      301 tcttgcgcat cgacctcggg cgctacatca cgagccggca cgccggctcc gcgcgccgca
      361 tcgaggcgat cctgttcgag tccagcctgc tcgatcgcgt gctcgcggcc atcgagcgct
      421 ccgcgcgcgg caacctgctg ctgctgagcc cggcggtcgc gcaggacgtg cgcgaccagg
      481 tgcgcacgat cctcggcggc acgggcggcg cggtcgtcgc catcgtctcg tcggacgcgc
      541 gccgctacgt caagacgctg ctggagccga tcgcgcccga gctgcccgtg ctgtcctacc
      601 aggagctcga cgccgacgtc gcgctgcatc ccgtcggctg ggtcaccaat ccgcaggccg
      661 cctgatccct cccgtcgcca acgccgcctc ccgctcccat gacaccccag aacgtcctcg
      721 acatcacccg cgaaggcctg cagctggtct tctgggtctc cctgcccgtg gtgctggtgg
      781 ccgcgatcac ggcgctggtg gtggcgctcg ggcaggcggt cacgcagatc caggaccaga
      841 gcatcggcca gtcgatccgg ttgatctcgg tgatgctcac gctcgtcgtg ctcgcgggct
      901 ggctgggccg tacggtgctg ctgttcgccg agcgcgcgtt cgattcgctg gtgcggctgg
      961 catgaagcgc ctcttcggat tcctcgcagg cggcgcgttg gcgaccgcgt tgctctgcgc
     1021 cgcgccggcg gcgggcgcgg ccgagctgcc gtgggcgggc aagccctacc agatcgtcgc
     1081 caacgagaag ccgctggccg acctgctgcg cgagctcgcg gcctcgcagg gcaccaccgc
     1141 ggtgatcgac cccaaggtct cgggcacgat cagcggccgc ttcagcggct cgccgctgaa
     1201 gacgctcaac agcctgtgcg ccacctacgg gctgagctgg tactacgacg gctcgttcct
     1261 ctacgtcgac ccgtcggccg atgcgcgcag cgaggtgcta tcgatcggca acgccacggg
     1321 caacgcgagc tggctgatcc agtcgctcac gcagctgcgc atctacgact cgcgctatcc
     1381 gctgctggtc agcggccggg acggcaccgc gcgcgtgacg ggcccgaagc gctacgtcga
     1441 gatggtgcgc caggtggcgc ggctgctcga ttccaagagc gcgctcaacg acggcgcgga
     1501 gatccgcctg ttcccgctga agtacgcgtg ggcgggcgac ttcaagatca cgcgctcggg
     1561 caaggaggtc gcggtgccgg gcgtggccaa cacgctgcgc agcctgtact cccacgcgaa
     1621 cggcggctcg tccgcctccg gcaccgccgg cgcggtggcg cgccagtacg cggcctacca
     1681 ggcgggcgcg gatcggcagc tgaggctgtc cacgggcgac ctcgtgaacg cgccgatggt
     1741 gcagctgccg ggcctcgcgc cgccgatgac cggcggcgac gcgggcgacg ccggatcggc
     1801 gccggcggcc ggcctgtccg cgggcgacgg cggcctgccg cagttccagg ccgacacccg
     1861 cctgaacgcg gtgctggtgc gcgacctgcc gacgcacatg gcgcagtacg agaagctgat
     1921 cgccgcgatg gaccagcgtc cgcgcctggt cgagatcgag gtcacgatca tggacgtcag
     1981 caccgacacg ctcgacagcc tgggggtgga ctggcgcgcg cactcgtcgc acgtcgactt
     2041 ccagaccggc aacgccacga actcgccgct gacgctgggc ggcgcgactt ccgagtccgg
     2101 ccagaccggc ggcctgtgct gcaccaacgc caacggcctg ccgacgacgc cgctcggctc
     2161 gatcttcacc gcggcgatcg gcaacagcct gcgcgattac ctgctcgccc gcgtgagcgc
     2221 gctcgcgcag accggcaacg ccgacgtcgt cgcgcggccc aaggtgctca cgctggacaa
     2281 caacgaggcg gtgctcgaga acctgagcga cttctacgtg agcgtcgccg gcttccagga
     2341 cgcgagcctg ttcaaggtca cgaccggcac ctcggtgcgc gtgacgccga tgatcgtcga
     2401 cgagaacggc ggccgcggcg tgatgatgac gatcgacatc caggacggca acatcgaccc
     2461 gacgcagtcg gtgggccaga tcccggtcgt gcgccaccgc acggtcgaca cccaggcgct
     2521 gatcgacgag ggctcgagcc tgctgctggc gggctacacg tcggagtcga agtcgaccgc
     2581 ggcgtcgggc gtgccggtgc tctcgagcat tcccatcgtc ggcaacctgt tcaagtacaa
     2641 ccagaagcag ggcagcaagg aggaacgcct ctacctgctg acgccgcggc tcgtgacgcc
     2701 gggcctggcg gccaacgggg cggcgctcgc gccgctgccg acgcaggacc ggccatcgct
     2761 gccggtgcag gacgcgccgg tgccgccggc cgcggtgccg gtgtcctcgg cccacgccgg
     2821 gaggcgcacg tgaaggcgac gcccgagttc gtcacgcccg gctggtgcct gcgcttcctc
     2881 agcggcgcca tgaagggccg cacgatcgag ctgcggcccg gtgccaacgt cctcggctcg
     2941 gcgggcgagt gcgacgtgat gctgcccggc ggggacgtgc tgccgcgcca tctcgtcttc
     3001 aacgtcggcg agctcgtggt gtcggtgcag aggacgggcg agggcgaggt gctgctcaat
     3061 cgcgagccga tgcagcagcc ccgccgcagc ctggtcccgg gcgacgtggt gacggtgggc
     3121 cgcatcgaac ttcagttgga gcgcagctat cctgcggtcg accgcgccga cccgatgttc
     3181 gcggggccgg agagcgtcct gcccggcgac acccccgtgc acgtgccccc gtcgggtcgc
     3241 gggcgcggat tctgggcggg cgcggcgctg ctggtgctct cgtgcgcggg cctgctgggc
     3301 gtgggcctgt gggccggcgg cgaggatcgg ccggccgccg ccgccgatcc cgccgcggtc
     3361 gcgcgcgtga tcgcgccgtt caccgagctc gaggccgtcg cggcgcccgg cgggcaggtg
     3421 gtggtcaagg gcttcgtcga gtcgcgcctg cgccgccagc agctggagca ggcgctcgcg
     3481 cccatgggcc ggcgcgtgac cgtcgaggcg gtgccggtcg acgagctcgt cgagcaggcg
     3541 cggcgctacc tgggcgatcc cgcgctgggc gtgagctacg ccggcaaggg gcagctcgtg
     3601 gtcaccggcg cgaccgatga aggcgcgtcc cgcgaccgcc tgcgccggct cgccgaggac
     3661 ctgcacccgt cggtgctggt gacggaccgc gtctcgtggc gccacgcgcc cgcgccggac
     3721 cgcgacgcgg cgctgcgcgc gcactgggcg gcctggcagg acgcgctgcc gtcgcgcttg
     3781 gtcggcatca ccgaggacga ggacggcctg cgctcgatcc agctcgccga cggcacgcgc
     3841 tactacgagg gcgcgccgct gaagtcgggc gcgcagatcg cgcacatcgg cgcggacggc
     3901 ctcgtgctcg aaggaggcgc ccgtgacggc gcgaagtgaa caccggcggg gcgctgcggc
     3961 ggcccgcctc ccggattcgc ggcggctgcc tgccgcgaat acccgcccgg gcatctgtcc
     4021 ggcgggacat cgtcaaaccc actaggagaa aagcacatgt ccaccgtcga tacctccatc
     4081 gccgcgatgc aacaagccgc cgccgacagc gctcgcctgc aggccgccag catcagcgtg
     4141 cagacgcaga tccagggcgc gaccacgtcc gcccagaccg tcaacagcgc cagcgccgcc
     4201 gccaccgaga ccgccaaggg cgtcgccagc gacaccaagg cggccgcgcg cgcgtcctga
     4261 cgccgtgcgc ggcg
//
LOCUS       DASVLN010000392         2760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_128029, whole genome shotgun sequence.
ACCESSION   DASVLN010000392 DASVLN010000000
VERSION     DASVLN010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128029"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VF453_09785"
     CDS             <1..839
                     /locus_tag="VF453_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="disulfide isomerase"
                     /protein_id="HEX7687987.1"
                     /translation="RSRALMDVFTSSAPELVDALAPAPGAERAKLVAAADTALARMQA
                     DATLSRADRMTALQARVDLARIDQKKDALHPVLPPALVQEARAAAADADREVTSPFER
                     QAVITEASQVLADAGLWKESDALLQGSLGKSHSPYYLMSELGANARREGRPAEALQWY
                     RKAFERSEGPATRLQWGAAYLGALVDLAPQDVHAIEQAAHAVFAEAASQPGAFYERSG
                     RSLQRIGAKLAKWNASGRHQAAVDRLAAELHRTCAKLPAGDTARATCDSVLPGKASAT
                     AS"
     gene            849..2081
                     /gene="purT"
                     /locus_tag="VF453_09790"
     CDS             849..2081
                     /gene="purT"
                     /locus_tag="VF453_09790"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEX7687988.1"
                     /translation="MTNTKPLGTPLSAAATRVMLLGSGELGKEVLIALQRLGVETIAV
                     DRYEDAPGQQVAHHARTITMSDGAALRALIEREKPDLVVPEIEAIATPELQALEAEGV
                     VRVIPTARAARLTMDREGIRRLAAETLGLPTSPYVFCDSLQELQAAIDGTGAAGKPAI
                     GYPCIVKPVMSSSGKGQSRIDGPADVQAAWDYAMAGGRVSHGRVIVEGFIDFDYEITL
                     LTVRALEADGSVKTHFCAPIGHVQVKGDYVESWQPHRMAPKALEKSQAIAKAVTEDLG
                     GQGLFGVELFVKGDDVWFSEVSPRPHDTGMVTMITQWQNEFELHARAILGLPVDTSLK
                     SPGASAVIYGGVDAKAVQFDGVADAMRLPGVELRLFGKPESYVRRRMGVALAHHEDVE
                     RAREVAKEAAGKVRPKAA"
     gene            complement(2109..2435)
                     /locus_tag="VF453_09795"
     CDS             complement(2109..2435)
                     /locus_tag="VF453_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687989.1"
                     /translation="MESSKRRRPTVSPVVFKGLRFEALRRPRDHGSAQSGGALAAFDE
                     KSNKYLWGVQLYTTVFDEREERDVQEVYIKELSVSAGVDAVLATDELDRRWSVDIVTH
                     EVTPAR"
     gene            complement(2568..>2760)
                     /locus_tag="VF453_09800"
     CDS             complement(2568..>2760)
                     /locus_tag="VF453_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687990.1"
                     /translation="TASTRDPAPLTTGTRRLRSSDSPWQTLTSLRAQFNNAVLQFGFE
                     TALYIAQLADIAGKFRERY"
BASE COUNT          449 a          972 c          964 g          375 t
ORIGIN      
        1 cccgcagccg cgcgctgatg gacgtcttca ccagttcggc gcccgagctc gtcgacgcgc
       61 tcgcgcccgc gccgggcgcc gagcgcgcga agctcgtcgc cgcggccgac accgcgctcg
      121 cacgcatgca ggccgatgcg acgctctcgc gcgccgatcg catgaccgcg ctgcaggcgc
      181 gcgtcgacct cgcccgcatc gaccagaaga aggacgcgct gcatcccgtg ctgccgccgg
      241 cactggtgca ggaagcgcgc gccgcggcgg ccgacgccga tcgcgaggtg accagcccgt
      301 tcgagcgcca ggccgtcatc accgaggcga gccaggtgct cgcggacgcc ggcctgtgga
      361 aggagtccga cgcgctgctg cagggcagcc tgggcaagtc gcattcgccg tactacctca
      421 tgagcgagct cggcgccaac gcgcgccgcg agggccgccc cgccgaggcg ctgcagtggt
      481 accgcaaggc cttcgagcgc agcgagggcc ccgccacccg cctgcagtgg ggcgccgcct
      541 acctgggcgc gctggtcgac ctcgcgccgc aggacgtgca cgccatcgaa caggccgccc
      601 acgccgtctt cgccgaagcc gcgagccagc ccggcgcgtt ctacgagcgc agcggccgct
      661 cgctgcagcg catcggcgcg aagctcgcga agtggaacgc ttcgggcagg caccaggccg
      721 ccgtcgatcg cctcgccgcc gagctccacc gcacctgcgc gaagctgccc gcgggcgaca
      781 cggcgcgcgc gacctgcgac tcggtgttgc cgggcaaggc ctcagcaacc gcttcgtaga
      841 atcgcgccat gacgaacacg aaacccctcg gcacgccgct cagcgcggcg gccacgcgcg
      901 tgatgctgct tggaagcggc gagctcggca aggaagtgct gatcgcgctg cagcgcctgg
      961 gcgtcgagac catcgccgtc gaccgctacg aggacgcgcc cggccagcag gtcgcgcacc
     1021 acgcgcgcac gatcaccatg agcgacggcg ccgcgctgcg cgcgctgatc gagcgcgaga
     1081 agcccgacct cgtcgtcccc gagatcgagg ccatcgcgac gcccgagctg caggcactcg
     1141 aggccgaggg tgtcgtgcgc gtcatcccga ccgcccgcgc cgcgcgcctg accatggatc
     1201 gagagggcat ccgccgcctc gccgccgaga cgctgggcct gccgaccagc ccctacgtct
     1261 tctgcgactc gctgcaggag ctgcaggccg ccatcgacgg caccggcgcg gcgggcaagc
     1321 cggcgatcgg ctatccgtgc atcgtcaagc cggtgatgtc gagctccggc aagggccaga
     1381 gcaggatcga cgggccggcc gacgtgcagg ccgcgtggga ctacgcgatg gccggcggcc
     1441 gcgtgagcca cggccgcgtg atcgtcgagg gcttcatcga cttcgactac gagatcacgc
     1501 tgctgaccgt gcgcgccctg gaggccgacg gcagcgtgaa gacgcatttc tgcgcgccca
     1561 tcggccacgt gcaggtcaag ggcgactacg tggaaagctg gcagccgcac cgcatggccc
     1621 ccaaggcgct cgagaagtcg caggccatcg cgaaggcggt caccgaggac ctcggcgggc
     1681 agggcctctt cggcgtggag ctgttcgtca agggcgacga cgtctggttc agcgaggtca
     1741 gcccgcgccc gcacgacacc ggcatggtca cgatgatcac gcagtggcag aacgagttcg
     1801 agctgcacgc ccgcgccatc ctcggcctgc cggtggacac ctcgctcaag agccccggcg
     1861 cgagcgccgt gatctatggc ggcgtcgacg cgaaggccgt gcagttcgat ggcgtcgccg
     1921 acgcgatgcg cctgccgggc gtcgagctgc gcctgttcgg caagcccgag agctacgtcc
     1981 gccgccgcat gggcgtcgcg ctggcgcacc acgaggacgt ggagcgcgcg cgcgaggtgg
     2041 ccaaggaggc cgcgggcaag gtgcggccga aggcggcgtg agcgagtcgt ttgcgcgcca
     2101 cgcttacatc agcgagcggg cgtcacctcg tgagtcacga tgtctaccga ccatctccga
     2161 tcgagctcgt cggtcgccag cacggcatca acgcccgcgg aaaccgaaag ctccttgatg
     2221 tagacctcct ggacgtcgcg ctcttccctt tcatcgaaga cggtcgtgta cagctggacg
     2281 ccccagaggt atttgttcga tttctcgtcg aaggccgcca aagcgccgcc actctgcgcg
     2341 gaaccgtggt ctcgcggccg ccgcagcgcc tcgaagcgga ggcctttgaa gacaacgggg
     2401 gaaacggtcg ggcggcgacg ctttgaagat tccacagcgt attccttggt cgatgcagtc
     2461 gctcgcgcag cggccgcgtc gaagagggcg aggatgacaa ggccgctccc tgtcgcaaac
     2521 gtcggcagcg cgaagacgaa cggtggcgat ccgtcgtgcg cgcgtgatca atagcgctcc
     2581 cggaatttcc ctgcgatatc ggccagctgc gcaatgtaga gggcggtttc aaatccgaac
     2641 tgcaacaccg cgttattgaa ctgagctcgg agacttgtga gagtttgcca gggtgagtcc
     2701 gaagacctca agcggcgtgt gccagtcgtg agtggcgcgg ggtcgcgtgt tgaggctgtc
//
LOCUS       DASVLN010000393         5286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_341911, whole genome shotgun sequence.
ACCESSION   DASVLN010000393 DASVLN010000000
VERSION     DASVLN010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341911"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1553)
                     /gene="guaA"
                     /locus_tag="VF453_09805"
     CDS             complement(<1..1553)
                     /gene="guaA"
                     /locus_tag="VF453_09805"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7687991.1"
                     /translation="MHDKILILDFGSQVTQLIARRVREAHVYCEIHPNDVSDDFVRAF
                     APKGVILSGSHASTYEDAELRAPQAVWELGVPVLGICYGMQTMAAQLGGKVEYSDHRE
                     FGYAEVRAHGRTRLLDGIEDFVTPEGHGMLKVWMSHGDKVTELPPGFQLMASTGSCPI
                     AAMADEARGYYAVQFHPEVTHTAQGRAILERFVLKIAGANPDWVMTNHVEEAVQRIRE
                     QVGDEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLDEGKMVMDMFAGRL
                     HARVIHVDASAQFLGHLAGVSDPEQKRKIIGREFVEVFQAEAAKLKAAGAGHKGAKWL
                     AQGTIYPDVIESGGAKTKKATTIKSHHNVGGLPETLGLKLLEPLRDLFKDEVRELGVA
                     LGLPPEMVYRHPFPGPGLGVRILGEVKREYADLLRRADAIFIEELRNWKDGETGKSWY
                     ELTSQAFAVFLPVKSVGVMGDGRTYDYVVALRAVQTSDFMTADWAELPYGLLKKVSGR
                     IINEVRGINR"
     gene            complement(1576..2874)
                     /locus_tag="VF453_09810"
     CDS             complement(1576..2874)
                     /locus_tag="VF453_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7687992.1"
                     /translation="MTADPDPAAEDRADPVAAPAARARQAAMPFIMLTVLIDMVSIGL
                     IIPVLPRLVGSFERSAGDLAGAYALVAAAFGFANFLGSPVLGALSDRFGRRPVLLIGF
                     SGLAFSFFATAAATALWMLVAIRLVSGALQANIAVANAYVSDITPPEDRARRFGMLGA
                     MFGLGFILGPVTGGLLSGIDLRLPFWCAGGLAICNWLYGFFVLPESLPPARRQPFSWG
                     RANPVGSLLQLSRMRHLAALLAVVALSAMAQFILQSTWALYTQIKFGWTSMQVGMSLG
                     AVGVMSVIVQGALVGRMTRHFGAARVAVIGLASSAACYVAWGAATQGWMMYAVIACNV
                     FGFAASTALQSIVANAADPSAYGRTMGTVSGLSSLMAAVAPAIGPLLLKSVADAPRGD
                     WRLGAPFYVCAALQAVAMAMTLVHIRHHRPAEPATPAAAS"
     gene            complement(2994..4460)
                     /gene="guaB"
                     /locus_tag="VF453_09815"
     CDS             complement(2994..4460)
                     /gene="guaB"
                     /locus_tag="VF453_09815"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7687993.1"
                     /translation="MRLLGKALTFDDVLLVPAYSQVLPRDTSLRTHLSRNIVLNLPVV
                     SAAMDTVTEARLAIAIAQEGGIGVIHKNLTPMQQAAEVARVKRYESGLLRDPVTIAPG
                     ARVRDVVELSRLHGVSGFPVVDEGRVVGIVTNRDLRFETRMDAPVREIMTPQERLITV
                     PEGTSLAVAKELMHRHKLERVLVVNDAFELRGLFTVKDITKQTTFPNAARDAHGKLRV
                     AAAVGVGPGTEERVEALAKAGVDAIVVDTAHGHSQGVIDRVKWVKKNFPQVDVIGGNI
                     ATGAAALALAEAGADAVKVGIGPGSICTTRIVAGVGVPQVMAIDNVATALQGTGVPLI
                     ADGGIRYSGDIAKAIAAGASTVMMGGMFAGTEEAPGEVILYQGRSYKSYRGMGSIGAM
                     KAGSADRYFQENDDQPNADKLVPEGIEGRVPYKGSVVSILFQMAGGLRAAMGYCGCAT
                     IDEMRSRAEFVEITSAGIRESHVHDVQITKEAPNYRAD"
     gene            4510..5130
                     /locus_tag="VF453_09820"
     CDS             4510..5130
                     /locus_tag="VF453_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX7687994.1"
                     /translation="MLGRVARRSFEPAGDGRNTWLSLHLVTTAGHYTSRVSDLPCAAM
                     SKTAMPLVAALLVLAAAGASAQNVYKWRDASGVMHISDTPPPVGAARAVTQGNIPGAG
                     AVVNPAGASAASGAASETDLQKKKAQADAAKAEAAAAEKARVEKQKAAARAQNCAAAQ
                     EQARVMGSGQRVATVNAKGEREFLDDAAVAARQKQAQQMIAENCGK"
BASE COUNT          777 a         1851 c         1912 g          746 t
ORIGIN      
        1 cggttgatgc cgcgcacctc gttgatgatg cggcccgaga ccttcttcag caggccgtag
       61 ggcagctcgg cccagtcggc ggtcatgaag tcgctggtct gcacggcgcg cagcgcgacc
      121 acgtagtcgt aggtgcggcc gtcgcccatc acgcccacgc tcttgaccgg caggaagacc
      181 gcgaaggcct ggctggtgag ctcgtaccag gacttgcccg tctcgccgtc cttccagttg
      241 cgcagttcct cgatgaagat cgcgtcggcg cggcgcagca ggtcggcgta ctcgcgcttg
      301 acttcgccca ggatgcgcac gcccaggccc gggcccggga acgggtggcg gtagaccatc
      361 tcgggcggca ggcccagcgc gacgccgagc tcgcgcacct cgtccttgaa caggtcgcgc
      421 agcggctcga gcagcttcag gcccagcgtc tcgggcaggc cgccgacatt gtggtggctc
      481 ttgatcgtcg tcgccttctt ggtcttggcg ccgcccgact cgatgacgtc cggatagatc
      541 gtgccctgcg caagccactt cgcgcccttg tggccggcgc ccgcggcctt gagcttggcc
      601 gcctcggcct ggaacacctc gacgaattcg cggccgatga tcttgcgctt ctgctcggga
      661 tcgctcacgc ccgcgagatg gccgaggaac tgcgcgctcg cgtcgacgtg gatcaccctc
      721 gcgtgcaggc gcccggcgaa catgtccatc accatcttgc cctcgtccag gcgcagcagg
      781 ccgtggtcga cgaacacgca ggtgagctgg tcgccgatcg cgcgatggat cagggccgcg
      841 gcgacgctcg agtcgacgcc gcccgacagg cccaggatca cctcctcgtc gccgacctgc
      901 tcgcggatgc gctgcacggc ctcctcgacg tggttggtca tgacccagtc ggggttggcg
      961 ccggcgatct tcagcacgaa gcgctcgagg atcgcgcgcc cctgcgccgt gtgcgtcacc
     1021 tcggggtgga actgcaccgc gtagtagccg cgcgcctcgt cggccatcgc ggcgatgggg
     1081 cagctgccgg tcgaggccat cagctggaag cccgggggca gctcggtgac cttgtcgccg
     1141 tggctcatcc agaccttgag catgccgtgg ccctcgggcg tcacgaagtc ctcgatgccg
     1201 tccagcagcc gcgtgcgacc gtgcgcgcgc acctccgcgt agccgaactc gcggtggtcg
     1261 ctgtactcca ccttgccgcc cagctgcgcc gccatcgtct gcatgccgta gcagatgccc
     1321 agcacgggca cgcccagctc ccagacggcc tgcggcgcgc gcagctcggc gtcctcgtag
     1381 gtgctcgcgt ggctgcccga gaggatcacg cccttgggcg cgaacgcgcg cacgaagtcg
     1441 tcggagacgt cgttcggatg gatctcgcag tacacgtggg cctcgcgcac gcgacgcgcg
     1501 atgagctggg tgacctggga gccgaagtcg aggatgagga tcttgtcgtg catggcgtcg
     1561 gacggttgcg ggagttcagg aagcggcggc gggcgtcgcg ggctcggcgg ggcgatggtg
     1621 acggatgtgg acgagggtca tcgccatcgc gacggcctgc agcgcggcgc agacgtagaa
     1681 cggcgcgccg agccgccagt cgccgcgcgg cgcgtcggcg acggacttca gcagcagcgg
     1741 gccgatcgcg ggcgcgacgg cggccatcag gctcgacagg ccgctcaccg tgcccatcgt
     1801 gcgcccgtag gcgctcgggt cggcggcgtt ggcgacgatg ctctgcagcg cggtgctcgc
     1861 ggcgaagccg aagacgttgc acgcgatcac ggcgtacatc atccagccct gcgtcgccgc
     1921 gccccaggcg acgtagcacg cggccgagga cgcgaggccg atcaccgcca cgcgcgccgc
     1981 gccgaaatgc cgcgtcatgc ggccgaccag cgcgccctgc acgatcaccg acatgacgcc
     2041 caccgcgccg agcgacatcc ccacctgcat cgaggtccag ccgaacttga tctgcgtgta
     2101 cagcgcccag gtggactgca ggatgaactg cgccatcgcg ctgagggcga cgaccgccag
     2161 cagcgccgcg agatggcgca tgcgcgagag ctgcagcagc gagcccacgg gattggcgcg
     2221 cccccagctg aagggctggc ggcgcgcggg gggcagcgac tccggcagca cgaagaagcc
     2281 gtagagccag ttgcagatcg ccaggccgcc ggcgcaccag aacggcaggc gcaggtcgat
     2341 gccgctcagc aggccgccgg tgaccgggcc gaggatgaag ccgaggccga acatcgcgcc
     2401 cagcatgccg aagcggcgcg cgcgatcctc gggcggcgtg atgtcggaga cataggcgtt
     2461 ggcgaccgcg atgttggcct gcagcgcgcc gctcacgaga cggatcgcga ccagcatcca
     2521 cagcgccgtc gccgcggcgg tcgcgaagaa gctgaacgcg aggccgctga agccgatcag
     2581 cagcaccggc cggcggccga agcggtcgga cagcgcgccg agcaccggcg agccgaggaa
     2641 gttggcgaag ccgaaggccg ccgcgacgag cgcgtaggcg cccgcgagat cgcccgcgct
     2701 gcgctcgaac gagccgacca gccgcggcag taccgggatg atcaggccga tcgacaccat
     2761 gtcgatcagc accgtcagca tgatgaaggg catcgccgcc tggcgcgcgc gcgcggcggg
     2821 cgcggcgacg gggtcggcgc ggtcctcggc ggcgggatcg ggatcggcgg tcacttcggg
     2881 tcggtgcggt cggccgggcg gtggagggat cggtcctcgc ggcggaggcg gctggaaacg
     2941 agaaccgccc gggacgcgtg gcgccggccc gggcggttgc tcctgagacg ccgtcagtcg
     3001 gcgcggtagt tcggggcttc cttggtgatc tgcacgtcgt ggacgtggct ttcgcggatg
     3061 ccggccgacg tgatctcgac gaactcggcg cgcgaacgca tctcgtcgat cgtcgcgcag
     3121 ccgcagtaac ccatcgccgc gcgcaggccg ccggccatct ggaacaggat cgagaccacc
     3181 gagcccttgt aggggacgcg gccctcgatg ccctccggca cgagcttgtc ggcgttgggc
     3241 tggtcgtcgt tctcctggaa gtagcgatcc gccgagccgg ccttcatcgc gccgatcgag
     3301 cccatgccgc ggtagctctt gtagctgcgg ccctggtaca ggatgacctc gcccggcgct
     3361 tcctccgtgc cggcgaacat gccgcccatc atcacggtgc tcgcgcccgc ggcgatggcc
     3421 ttggcgatgt cgcccgagta gcggatgccg ccgtcggcga tcagcggcac gccggtgccc
     3481 tgcagcgcgg tcgcgacgtt gtcgatcgcc atcacctgcg gcacgcccac gcccgcgacg
     3541 atgcgcgtcg tgcagatcga gcccgggccg atgccgacct tgaccgcgtc ggcgccggcc
     3601 tcggccagtg ccagcgcggc cgcgccggtg gcgatgttgc cgccgatcac gtcgacctgc
     3661 gggaagttct tcttgaccca cttcacgcgg tcgatcacgc cctggctgtg gccgtgcgcg
     3721 gtgtcgacga cgatcgcgtc gacgccggcc ttggcgagcg cctcgacgcg ctcctcggtg
     3781 cccgggccca cgccgacggc cgcggccacg cgcagcttgc cgtgggcgtc gcgggcggcg
     3841 ttcgggaagg tggtctgctt ggtgatgtcc ttgacggtga acaggccgcg cagctcgaag
     3901 gcgtcgttga cgaccagcac gcgctccagc ttgtggcggt gcatcagctc cttggccacc
     3961 gcgagcgagg tgccctcggg caccgtgatc aggcgctcct gcggcgtcat gatctcgcgc
     4021 acgggggcgt ccatgcgggt ctcgaagcgc aggtcgcggt tggtgacgat gccgaccacg
     4081 cggccctcgt cgaccaccgg gaagcccgag acgccgtgca ggcgcgagag ctcgacgacg
     4141 tcgcgcacgc gggcgccggg ggcgatcgtc acggggtcgc gcaggaggcc ggactcgtag
     4201 cgcttcacgc gcgccacctc ggcggcctgc tgcatgggcg tgaggttctt gtggatgacg
     4261 ccgatgccgc cttcctgcgc gatcgcgatc gccaggcggg cctccgtcac ggtgtccatc
     4321 gccgcggaga ccacgggcag gttcaggacg atgttgcggc tcaggtgggt tctgaggctg
     4381 gtgtcgcggg gcaacacctg ggaatatgcc ggcaccaaca acacgtcgtc gaaggtgagc
     4441 gctttgccta gaaggcgcat ggggggagct ccagacgcaa aagcggatta tacggatcgg
     4501 cggctccgcg tgctcggccg cgtggcgcga aggtctttcg aacccgccgg cgatggccgg
     4561 aacacctggc tttctttgca tttggtcacc acggccgggc actacacttc gagggtttcc
     4621 gacctccctt gcgccgccat gtccaagacc gccatgcccc tcgtcgccgc cctgctggtg
     4681 ctcgccgccg ccggcgcgtc cgcacagaac gtctacaagt ggcgcgacgc gagcggcgtg
     4741 atgcacatca gcgacacgcc gccgccggtg ggcgctgcgc gcgccgtgac gcaaggcaac
     4801 atccccggcg caggggccgt cgtcaatccg gccggcgcct cggcggccag cggggccgcc
     4861 agcgagaccg acctgcagaa gaagaaggcc caggcagacg ccgccaaggc agaggccgcc
     4921 gccgcggaga aggcgcgcgt cgagaagcag aaagccgccg cccgcgcgca gaactgcgcg
     4981 gccgcgcagg agcaggcccg cgtgatgggc agcggccagc gcgtcgcgac ggtgaacgcc
     5041 aagggcgagc gcgaattcct ggacgacgcc gctgtcgccg cacgtcagaa gcaggctcag
     5101 cagatgatcg ccgagaactg cggcaagtga ggccgcggcc gcctcagccg ggcgccgctc
     5161 cgcccttcgg cggcttcgcg ttgcgcgcgc ggatcgcctg cacgccgccc gacttgcgat
     5221 agcgcgcgcg gcgggcgtcc atcggcgcca cctgcaggcc gcgcaggagc tccagccggt
     5281 cgccgt
//
LOCUS       DASVLN010000394         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_181905, whole genome shotgun sequence.
ACCESSION   DASVLN010000394 DASVLN010000000
VERSION     DASVLN010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181905"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..993
                     /gene="cysD"
                     /locus_tag="VF453_09825"
     CDS             94..993
                     /gene="cysD"
                     /locus_tag="VF453_09825"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004694369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEX7687995.1"
                     /translation="MNLTHLQRLEAESIHILREVVAEADNPVMLYSIGKDSAVMLHLA
                     RKAFFPAPPPFPLLHVDTTWKFRAMYEMRDRMARESGMELLVHQNPEAAELGINPFTH
                     GSAIHTDMWKTQGLKQALDKWNFDAAFGGARRDEEKSRAKERIFSFRSAQHRWDPKNQ
                     RPELWHLYNGRKMKGESIRVFPISNWTELDIWQYIHLENIPIVPLYFAQERPVVNRDG
                     TLIMVDDDRMPLKAGEVPMMKSVRFRTLGCYPLSGAVESTADTLPAVIQEMLLTKTSE
                     RQGRMIDHDSAASMEKKKQEGYF"
     gene            1149..3053
                     /gene="cysN"
                     /locus_tag="VF453_09830"
     CDS             1149..3053
                     /gene="cysN"
                     /locus_tag="VF453_09830"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEX7687996.1"
                     /translation="MAHQSDLIATDIEKYLKQHERKSLLRFITCGSVDDGKSTVIGRL
                     LYESKMLFEDQLAAIENDSKKWGTQGGEIDFALLVDGLAAEREQGITIDVAYRFFSTD
                     RRKFIVADTPGHEQYTRNMITGASTADVAVILIDARKGVLTQTRRHSYLVSLIGIRKV
                     VLAINKMDLVDYSQKVFDKIDEEYRAFAKQIGLEDITSIPLSGLKGDNMLVASDKTPW
                     YRGPTLMGFLETCEVDETRLQQETFRMPVQWVNRPNLDFRGFAGLIASGTVRPGDRVV
                     AQPSGQESRVARIVAMGGDLPVAVAGQSVTLTLEDEIDVSRGDVLSKAEAPAEVADQF
                     EASLVWMSDEPMLPGRPYLMKIGTQTVSASITEPKYKVNVNTMEQLAAKQLGVNEIGV
                     VKLALDRPIAFDPYKANRDTGGFILINRMNNNTVGAGMLNFALHRSHNIPLQHVDVDK
                     ALRSQLKAQKPAVLWFTGLSGAGKSTIANLVEKKLAAMGRHTYLLDGDNVRHGLNKDL
                     GFTEADRVENIRRIAEVSRLMVDAGLIVLTAFISPFRSERAMARGQMAPGEFIEIHVN
                     TPLAVAEERDAKGLYRKARRGEIANFTGISSPYEPPEQPEITVNTDTMTSEQAADQII
                     ALLQARGLIG"
BASE COUNT          577 a         1089 c         1027 g          438 t
ORIGIN      
        1 gttgcggccc tcgcggccgc gcccgccgcc gcgcgcccgc gcccggccgc gcccccgact
       61 tccccttccc ctgcgcccat cacggacgaa tccatgaacc tcacccatct ccagcgcctg
      121 gaagccgaga gcatccacat cctgcgcgag gtcgtcgccg aggccgacaa cccggtcatg
      181 ctctattcga tcggcaagga ctcggccgtc atgctgcatc tggcgcgcaa ggcgttcttc
      241 cccgcgccgc cgccgttccc gctgctgcac gtcgacacca cgtggaagtt ccgcgcgatg
      301 tacgagatgc gcgaccgcat ggcgcgcgaa agcggcatgg agctgctggt gcaccagaac
      361 cccgaggccg cggagctggg catcaacccg ttcacgcacg ggtcggcgat ccacaccgac
      421 atgtggaaga cgcaggggct gaagcaggcg ctggacaagt ggaacttcga cgccgcgttc
      481 ggcggcgcgc gccgcgacga ggagaagtcg cgcgcgaagg agcgcatctt ctcgttccgc
      541 tcggcgcagc atcgctggga tccgaagaac cagcggcccg agctctggca cctgtacaac
      601 gggcgcaaga tgaagggtga gtcgatccgc gtgttcccga tctcgaactg gacggagctc
      661 gacatctggc agtacatcca cctcgagaac atccccatcg tgccgctgta cttcgcgcag
      721 gagcgtcccg tcgtgaaccg tgacggcacg ctgatcatgg tcgacgacga ccgcatgccg
      781 ctgaaggccg gcgaggtgcc gatgatgaag agcgtgcgtt tccgcaccct cggctgctac
      841 ccgctgtcgg gggccgtcga atcgaccgcc gacacgctgc ccgcggtgat ccaggagatg
      901 ctgctgacga agacctccga gcgacagggc cgcatgatcg accatgactc ggccgcctcg
      961 atggagaaga agaagcaaga ggggtacttt tgagccccac ggtcgctggc gctccctgcc
     1021 cccgaggggc cggccgcgag tcgaccggcg aagccggatc gaccgctccc ttgaacttca
     1081 tgcgaatcca atgagcggca gcgccggata cgaacgactc cgcacgccga actgactacg
     1141 gatacaagat ggcccaccaa tccgacctga tcgccaccga catcgagaag tacctgaagc
     1201 agcacgagcg caagagcctg ctgcgattca tcacctgcgg ctcggtggac gatggcaagt
     1261 ccacagtgat cgggcggctg ctgtacgaat cgaagatgct gttcgaggac cagctcgccg
     1321 cgatcgagaa cgactcgaag aagtggggca cgcagggcgg cgagatcgac ttcgcgctgc
     1381 tcgtcgacgg cctggccgcc gagcgcgagc agggcatcac gatcgacgtc gcctaccgct
     1441 tcttctccac cgaccgccgc aagttcatcg tcgccgacac gcccggccac gagcagtaca
     1501 cgcgcaacat gatcaccggc gcgtcgacgg ccgacgtcgc ggtcatcctc atcgacgcgc
     1561 gcaagggcgt gctgacgcag acccgccgcc acagctacct cgtgagcctg atcggcatcc
     1621 gcaaggtggt gctggcgatc aacaagatgg acctggtcga ctactcgcag aaggtgttcg
     1681 acaagatcga cgaggaatac cgtgcgttcg cgaagcagat cgggctggag gacatcacgt
     1741 cgatcccgct gtcgggcctg aagggcgaca acatgctggt ggcgagcgac aagacgccgt
     1801 ggtaccgcgg cccgacgctg atgggcttcc tcgagacctg cgaggtcgac gagacccgcc
     1861 tgcagcagga gaccttccgc atgccggtgc agtgggtcaa ccgccccaac ctcgacttcc
     1921 gcggcttcgc cgggctgatc gcgagcggca cggtgcggcc cggcgaccgt gtcgtcgcgc
     1981 agccctcggg ccaggagagc cgcgtcgcgc gcatcgtcgc gatgggcggc gacctgcccg
     2041 tggcggtcgc cggccagtcg gtgacgctga ccctggagga cgagatcgac gtctcgcgcg
     2101 gcgacgtgct gtccaaggcc gaggcgcccg ccgaggtcgc cgaccagttc gaggcctcgc
     2161 tcgtgtggat gtccgacgag cccatgctgc cgggccggcc ctacctgatg aagatcggca
     2221 cgcagaccgt cagcgcgtcc atcacggagc cgaagtacaa ggtcaacgtc aacacgatgg
     2281 agcagctggc ggccaagcag ctgggcgtca acgagatcgg cgtcgtcaag ctcgcgctcg
     2341 accggccgat cgccttcgac ccgtacaagg ccaaccgcga caccggcggc ttcatcctca
     2401 tcaaccggat gaacaacaac acggttggcg ccggcatgct gaacttcgcg ctgcaccgct
     2461 cgcacaacat cccgctgcag cacgtggacg tggacaaggc gctgcgctcg cagctgaagg
     2521 cgcagaagcc cgcggtgctg tggttcaccg ggctgtcggg cgcgggcaag tcgacgatcg
     2581 ccaacctggt cgagaagaag ctcgccgcga tggggcgcca cacctacctg ctggacggcg
     2641 acaacgtgcg ccacggcctg aacaaggacc tcggcttcac ggaggccgac cgcgtcgaga
     2701 acatccgccg catcgcggag gtctcgcggc tgatggtcga cgccggcctg atcgtgctga
     2761 cggccttcat ctcgccgttc cgctccgagc gcgcgatggc gcgcggccag atggcgccgg
     2821 gcgagttcat cgagatccac gtgaacacgc cgctcgcggt ggccgaggag cgcgacgcca
     2881 agggcctcta ccgcaaggca cgccgcggcg agatcgccaa cttcacgggc atcagctcgc
     2941 cctacgagcc gcccgagcag cccgagatca cggtcaacac ggacacgatg acgtcggagc
     3001 aggcggccga ccagatcatc gcgctgctgc aggcgcgcgg cctcatcggc tgaagtcgcg
     3061 ctcgaggacg cgcacgcccg tcacgccggc tccggcgacg gccagcgcgg ccgcctcgcc
     3121 gggtgcggcg t
//
LOCUS       DASVLN010000395         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_128345, whole genome shotgun sequence.
ACCESSION   DASVLN010000395 DASVLN010000000
VERSION     DASVLN010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128345"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF453_09835"
     CDS             <1..211
                     /locus_tag="VF453_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX7687997.1"
                     /translation="PQQSLELWALPRAGAPKSLGLIASDRLTALSRRALPADTKGLAV
                     SLEPLGGSPTGAPTGPVLFVGDLTL"
     gene            complement(242..1300)
                     /locus_tag="VF453_09840"
     CDS             complement(242..1300)
                     /locus_tag="VF453_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7687998.1"
                     /translation="MSNWEDMTGPLRGPDADTDTGPALAEMSSTKKLAKCVDVTGKVL
                     TTFPFVNPLLAKAIPFITKGFRSESDVVEAAKHVDDSTFSEVPWDVTSFVSNATASVL
                     ALSGTSEMLFARNLKQTLLPIAQSAVITHVAQNSTMASLFRGVAWGEPFISPFGKAEI
                     RAAMDSEIIGRAHNRFVESVMEGKPAAQELVSHYREIIQNVGKTAFFNVPEFPAERAA
                     AALETAAHEVRGLASDAGKLAKFNKAPVVKKLAANVLPGASVVLDGYALHKSWQAYEE
                     AGDTGARPDAMASVLCSTVAMTGSVLNAVGMNTRIAPRLPPTLQGRLVTVGLALMFGG
                     AACAYALERRVVHKQHTK"
     gene            complement(1543..>2516)
                     /locus_tag="VF453_09845"
     CDS             complement(1543..>2516)
                     /locus_tag="VF453_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HEX7687999.1"
                     /translation="PPPPPPPSGHSLIGYWHDFTNPSGNTYPISQVTNDWDVIIVAFA
                     DNAGNGNISFTVDPNAGTEAQFIADVAAKRKAGKKVVLSVGGQNGSISLDNATMVTNF
                     VNSAYALIQKFGFDGIDLDLETGVSQGTALQANLVSAMQQLKAKVGPSFYLSMAPEHP
                     YVQGGYVAYGSIWGAYLPIIDALRNDLTEIHVQYYNNGGFTYDNASNQQVQEGTVDGL
                     VAGSLMLIEGFNVSYGTGWHFNGLRPDQVAFGVPSGPSSANTGFVTPAVITNTLNCLT
                     KLTGCNTIKPAKAYPTFRGAMTWSINWDRHDGYDFSVPTRAALNALP"
BASE COUNT          348 a          827 c          897 g          444 t
ORIGIN      
        1 gccgcagcag tcgctcgagc tgtgggcgct gccgcgggcc ggggcgccga agtcgctcgg
       61 cctgatcgcg tccgaccgcc tgaccgccct gtcgcgccgc gcgctgcccg ccgacaccaa
      121 gggcctggcc gtctcgctcg aaccgctggg tggctcgccc accggcgcgc ccacggggcc
      181 ggtgctgttc gtgggtgacc tgacgctgtg agctcgcgcg gcgcgggggc gttgcgcgcg
      241 ctcacttcgt gtgctgcttg tggacgacgc gtcgctcgag ggcataggcg caggccgctc
      301 cgccgaacat cagcgcgagg ccgacggtga ccaggcggcc ctgcaaggtg ggcggcagcc
      361 gcggggcgat gcgggtgttc atgccgaccg cgttcaggac ggacccggtc atcgcgaccg
      421 tgctgcacag gacggacgcc atcgcgtcgg gccgggcgcc tgtgtcgccc gcctcttcgt
      481 aggcctgcca cgacttgtgc agcgcgtagc cgtccaggac gacgctggcg cccggcagga
      541 cgttggctgc cagcttcttg acgaccgggg ccttgttgaa cttcgcgagc ttgccggcat
      601 cggacgccag tccccgcacc tcgtgcgcgg cggtctccag cgccgccgcg gcgcgctcgg
      661 cggggaactc cggcacgttg aagaatgccg tcttgccgac gttctggatg atctcgcgat
      721 agtggctgac cagctcctgc gccgcaggct tgccttccat caccgattcc acgaagcggt
      781 tgtgggcgcg gccgatgatc tccgaatcca tggcggcgcg gatctcggcc ttgccgaagg
      841 gcgagatgaa cggctcgccc caggcgacgc cgcggaacag gctcgccatc gtcgagttct
      901 gggcgacatg ggtgatgacg gcgctttgcg cgatcggcag cagtgtctgc ttgaggttgc
      961 gcgcgaagag catctcgctc gtgcccgaga gcgcgaggac gctggccgtc gcgttgctga
     1021 cgaagctggt cacgtcccat ggcacttcgg aaaaggtgct gtcgtcgaca tgcttggccg
     1081 cctcgaccac gtcggattcg ctgcggaagc ccttggtgat gaaggggatc gccttggcca
     1141 gcagcggatt gacgaagggg aacgtcgtca ggaccttgcc cgtgacatcg acgcatttcg
     1201 ccagcttctt cgtcgacgac atctccgcca gcgccgggcc ggtgtccgtg tcggcgtcgg
     1261 gccctctcag cgggccggtc atgtcttccc agttggacat cgaatctcct cctcgcggcg
     1321 cacggcggtc ctgctccctg cgaacgacgg ggcgcgagtc cgctcggcca gttgcccgaa
     1381 ggcccggcct gcattcagtc gtgcgctcat accctacccc gtacggtaag cgactctacg
     1441 ccacttcgac caccgacggc ggcgggcgac gaaaaaaggg gcccgacgct cgcgcgccgg
     1501 gccctcggtc tcttccggga gtgcggggtc tcggccgaca ggtcagggca gcgcgttcag
     1561 cgccgcgcgc gtcggcacgg agaagtcgta cccgtcgtgc ctgtcccagt tgatcgacca
     1621 cgtcatcgcg ccgcggaagg tcgggtaggc cttcgccggc ttgatggtgt tgcagccggt
     1681 cagcttcgtg aggcagttca gcgtgttcgt gatcaccgcc ggcgtgacga agccggtgtt
     1741 ggccgagctc ggccccgagg gcacgccgaa cgccacctgg tcggggcgca ggccgttgaa
     1801 gtgccagccg gtgccgtacg acacgttgaa gccctcgatc agcatgaggc tgccggcgac
     1861 gagaccgtcg acggtgcctt cctgcacctg ctggttgctc gcgttgtcgt aggtgaatcc
     1921 gccgttgttg tagtactgga cgtggatctc ggtgaggtcg ttgcgcagcg cgtcgatgat
     1981 gggcaggtag gcgccccaga tcgagccgta ggcgacgtag ccgccctgca cgtacgggtg
     2041 ctccggcgcc atggacaggt agaacgacgg gccgaccttg gccttgagct gctgcatcgc
     2101 cgacaccagg ttcgcctgca gcgcggtgcc ttgcgagacg ccggtctcca ggtcgaggtc
     2161 gatgccgtcg aagccgaact tctggatcag cgcgtaggcg ctgttgacga agttggtcac
     2221 catcgtcgcg ttgtccagcg agatcgagcc gttctgcccg cccaccgaca gcaccacctt
     2281 cttgccggcc ttgcgcttgg cggcgacgtc ggcgatgaac tgcgcctcgg tgcccgcgtt
     2341 cgggtcgacg gtgaagctga tgttgccgtt gccggcgttg tcggcgaagg cgacgatgat
     2401 cacgtcccag tcgttggtca cctgcgagat agggtaggtg ttgccggacg ggttggtgaa
     2461 gtcgtgccag tagccgatca gcgagtggcc tgacggcggc ggcggcggcg gcggcg
//
LOCUS       DASVLN010000396         7545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_182036, whole genome shotgun sequence.
ACCESSION   DASVLN010000396 DASVLN010000000
VERSION     DASVLN010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182036"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="VF453_09850"
     CDS             <1..1938
                     /locus_tag="VF453_09850"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7688000.1"
                     /translation="ADAPLSPIAPAPGTPPAPKTAAPGTPGPLPAGTVTTAHPLTADD
                     LESWLDGLIPAALVTARTPGAVVAVVKDGQVLLEKGYGWADAEKHVPVDPKATLFRPG
                     STSKLFTWTAVMQLVEQGKLDLDTDVNRYLDFKVPVRNGHPLTLRHIMTQTSGFEEVI
                     KDLIAFDKAPPPLREVLVAEMPPLRADPGTTAGYSNYATALAGYIVQRVSGEPFEDYV
                     EHHIFQPLGMAHSTFRQPLPAALAPQMAQGYMETGQPGHGFEIVNLPPAGSLSATADD
                     MTRFMIAHLQDGRFGDQRILQAATAQTMHTTPTRLFPDLNGIALGFYQQDINGHRVVA
                     HGGDLNYFHTELALFIDDHVGLFFSVNAPGKEGMGEMLRVRLFNGFADRYFPHPETVT
                     PIDPATARAHAAMIAGHWVNTRRADSTFLKLVDLLSPTTVTANADGSLTTKVLIDPQT
                     FVETSPFLWHEVDGHDKLQARVKDGKVVGWTTDMLAFAWSYESPGGLAAAGLQQPACA
                     LAFAIVALAAAMWPISAVVRRRLAVPRAESVSLRRARRFADAGAALLIAAVVAWVGFF
                     AAVMSTTFTGLDPLLHLAQVLAIVGFGGGTLANAWLLAVQRRERLRKSALAWTAVRLA
                     ALAYLLSVAFGYNLLSLSGHY"
     gene            1954..3249
                     /locus_tag="VF453_09855"
     CDS             1954..3249
                     /locus_tag="VF453_09855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX7688001.1"
                     /translation="MSTTSPAAAPHARQADAQARRGHPPGLAVLFLTEMWEKFSFFGM
                     RALQVYYMTKQLHFSQASASLVYGAYAAGVYLTPIAGGVISDRWLGRHRAVVLGGLIM
                     ALGHFMMASEALFFPAMATIAIGNGLFLPNLPSQVGALYAEDDPRRASAYNLYYVGIN
                     LGATLAPLVCGTLGEVYGWHWGFGAAGVGMCLGLVVYLAGGRWLPHDGPRTAAPSTHT
                     PDDRAATRVGLLVAIGLAVVVYRVAYEQTGNTLAVWVDASVDRHFGGWLMPATWVQSL
                     NPLFVFLLTPPLVALWQRRELRRGAARPLARMATGAFVSALAWLGLAAVIHRDLAQHA
                     PTGIAWVTAFFALFTLAELYILPVGLGLFASLAPARFRATTIAAWFLAAFAGNLLSGV
                     LGTAWTHLAPDTFFVLTAGVAALAGAMLRAIGLLAEARR"
     gene            3266..3547
                     /locus_tag="VF453_09860"
     CDS             3266..3547
                     /locus_tag="VF453_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688002.1"
                     /translation="MAEDPRHAGPAPGRPYDLEQSALQLAALIRASLAVGHTRVALQR
                     SFMLLCLCGRLPEDLAPVVEDGLARRPLREIRRMKQAARAWAAMLGTRG"
     gene            complement(3587..5566)
                     /locus_tag="VF453_09865"
     CDS             complement(3587..5566)
                     /locus_tag="VF453_09865"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7688003.1"
                     /translation="MRRRGFAAWAALVAAAALAACGTSAAMDPGGAAAPAPGASASAP
                     DAAHALDAADLSAWLDGRVPYALKTGDIAGMVISVVKDGRVLLQKGYGYADVAARRPM
                     DPQTTLVRPGSTSKLFTWTAVMQLVQQGRLDLDRDVDDYLDFKVPEPYGKPVTMRDLM
                     NHRGGFEEGLKDVLSYDPARAQSTERYLKTHPRAMLFPPGTVPAYSNYGVALAGYIVQ
                     RVSGEAYDAYIARHVFEPLGMAHSTMAQPVPAPLRPSLAEGYRTASGPPSPFEFVVTA
                     PAGSASTTAADMARFMLAHLQQGRLDGHAILDPKTTALMHAPTESALPGFATMAHGFF
                     RSVQNGRTVIGHGGDTIVFHTELDLLPQEGVGIFFTFKSRGRDNAVYAARRELFDGFM
                     DRYFPAPSAPDPPALASARADAGRIAGRYQGSRRIEHGFLSLLYLLGQVVITANADGT
                     IVAPDPLGLGTQTFREVAPGLWRELGGSRQLRLAEVGGVKTVVDSEDATGVLQASPFA
                     SSAPLTLGVLAFSLLTMVLSVVYWVMAALARRAGGAPVAGNPRLATPRRLQRAAIVVD
                     ALYLAAWSAFIAPVLDTDIGAYNSGNDWIVRLLQASGLLAVAAAVAGAWAAWKLLRSD
                     ASRLTRAWAVVVAFALLGVVWVAAMGGLLRWDVNY"
     gene            5788..6516
                     /locus_tag="VF453_09870"
     CDS             5788..6516
                     /locus_tag="VF453_09870"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX7688004.1"
                     /translation="MNANGHLPGDAGSPGATIRALRLGKGLTLAQLSERTGLAISTLS
                     KLEKGSVSLSYDKLMLISRGLGVDMASLVDPKPPVPRAPALSAGRRVVQRAGEGQLVE
                     TRSYRQVYLATELLNKKMTPMFVEIRARTMEEFTAEFGGLIRHPGEEFTFVIEGELDF
                     HSELYAPLRLRAGDSIYFDSEMGHAYLKASEEPCRLVCSCAPRGDEDTMTEMFVSASE
                     RLAPAAAEPAPRAPRVQRARKRGG"
     gene            complement(6525..6944)
                     /locus_tag="VF453_09875"
     CDS             complement(6525..6944)
                     /locus_tag="VF453_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7688005.1"
                     /translation="MTTHFDTVPSTLNHLSFPSHDVPATAAFFERFLGFQVLAMGTSR
                     VLKKRSLDIVIEDARDRDVAWPGNFHLGIEVATRADLERMRADMLDAGVPMETDLFGH
                     ARGSRFFCWMPGRLMLEVNTREDAAPEFRRTFASQQG"
     gene            7124..>7545
                     /locus_tag="VF453_09880"
     CDS             7124..>7545
                     /locus_tag="VF453_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX7688006.1"
                     /translation="MPWHARLDLDYRTDAAGASGTPRTRLDFRHDGPLRILKSLHPEG
                     DAVCHSVIVHPPGGIVGGDTLDVSIALAPGAHALLTTPGATRFYRSAGARAAQAVDAR
                     LAEGARLEWLPLETLVHSGALADNRLRFDLAPGAETMG"
BASE COUNT         1032 a         2811 c         2620 g         1082 t
ORIGIN      
        1 gccgacgcgc ccctgtcgcc gattgcaccg gcacccggca cgccgcccgc gcccaagacc
       61 gccgcgcccg gcacgccggg gccgctgcct gccggcaccg tcacgaccgc gcacccgctg
      121 accgccgacg atctcgaatc ctggctcgac gggttgatcc ccgccgcgct cgtgaccgcg
      181 cgcacgcccg gcgcggtggt cgcagtcgtc aaggacggcc aggtgctgct ggagaaaggc
      241 tacggctggg ccgacgccga gaagcacgtc cccgtagacc cgaaggccac gctcttccgg
      301 cccggctcga cgtccaagct gttcacgtgg acggcggtga tgcagctggt ggagcagggc
      361 aagctcgacc tcgacaccga cgtcaaccgc tacctcgact tcaaggtccc ggtgcgcaac
      421 ggccatccgc tcacgctgcg ccacatcatg acgcagacga gcggcttcga ggaggtcatc
      481 aaggacctga tcgcgttcga caaggcgccg ccgccgctgc gcgaggtgct cgtggcggag
      541 atgccgccgc tgcgcgccga tccgggcacc acggccggct actcgaacta cgccaccgcg
      601 ctggcgggct acatcgtgca gcgcgtctcg ggcgagccgt tcgaggacta cgtcgagcac
      661 cacatcttcc agccgctggg catggcgcac tcgacgttcc gccagccgct gcccgccgcg
      721 ctcgcgccgc agatggcgca gggctacatg gagaccggcc agcccggcca cgggttcgag
      781 atcgtcaacc tgccgcccgc gggcagcctc agcgccaccg ccgacgacat gacccgcttc
      841 atgatcgcgc acctccagga tggacgcttc ggcgaccagc gcatcctcca ggcggcgacg
      901 gcgcagacga tgcacaccac gcccacgcgc ctgttccccg acctgaacgg catcgctctc
      961 ggcttctacc agcaggacat caacggccac cgcgtggtcg cgcacggcgg cgacctcaac
     1021 tacttccaca ccgagctcgc cctgttcatc gacgatcacg tcggcctgtt cttctcggtc
     1081 aacgcgccgg gcaaggaagg tatgggcgag atgctgcggg tgcgcctgtt caacggcttc
     1141 gccgatcgct acttcccgca tcccgagacg gtcacgccga tcgacccggc caccgcgcgc
     1201 gcgcatgccg cgatgatcgc cggccactgg gtcaacacgc ggcgcgcgga ctccaccttc
     1261 ctgaagctgg tcgacctgct ctcgccgacg accgtgaccg ccaatgccga cggctcgctg
     1321 acgaccaagg tgctgatcga cccgcagacc ttcgtcgaga cctcgccctt cctctggcac
     1381 gaggtcgacg gccacgacaa gctgcaggcg cgggtcaagg acggcaaggt ggtcggctgg
     1441 accaccgaca tgctcgcctt cgcgtggagc tacgagtcgc cgggcggcct tgccgcggcc
     1501 gggctgcagc agccggcgtg cgcgctcgcc ttcgcgatcg tggcgctcgc cgcggcgatg
     1561 tggccgatct ccgcggtcgt gcggcggcgc ctggccgtgc cgcgcgcgga gagcgtgtcg
     1621 ctgcgccggg cacggcgatt cgcggacgcc ggggcggcgc tgctgatcgc ggccgtcgtc
     1681 gcgtgggtcg gcttcttcgc cgcggtgatg tcgacgacct tcaccggcct cgacccgctg
     1741 ctgcacctgg cccaggtgct ggcgatcgtc ggcttcggcg gcggcacgct ggccaacgcg
     1801 tggctcctgg ccgtgcagcg ccgcgagcgc ctgcgcaaga gcgcgctcgc atggaccgcg
     1861 gtgcgcctgg cggccctcgc gtacctgctg tcggtcgcgt tcggctacaa cctgctgagc
     1921 ctcagcggcc actattgagc ggacgggacg cgcatgtcca cgacgagccc cgcggccgcg
     1981 ccgcacgccc ggcaggcgga cgcgcaggcg cgccgcggcc atccgcccgg cctggccgtg
     2041 ctgttcctga cggagatgtg ggagaagttc tccttcttcg gcatgcgcgc gctgcaggtc
     2101 tactacatga ccaagcagct gcacttctcg caggcttcgg cctcgctggt ctacggcgcc
     2161 tacgccgccg gcgtgtacct gacgccgatc gccggcggcg tgatctccga ccgctggctg
     2221 ggccgccacc gggcggtggt gctcggcggg ctgatcatgg cgctcggcca cttcatgatg
     2281 gccagcgagg cgctgttctt cccggcgatg gcgacgatcg cgatcggcaa cgggctgttc
     2341 ctgcccaacc tgccgagcca ggtcggcgcg ctctacgccg aggacgaccc gcgccgcgcg
     2401 tccgcctaca acctctacta cgtcggcatc aacctcggcg cgacgctcgc gccgctggtg
     2461 tgcgggacgc tgggcgaggt ctacggctgg cactggggct tcggtgccgc cggcgtcggg
     2521 atgtgcctgg gcctcgtggt ctacctcgcc ggcggccgct ggctgccgca cgacggcccg
     2581 cgcacggcgg cgccgtccac gcacacgccg gacgaccgcg cagccacccg cgtcggcctg
     2641 ctcgtcgcga tcggcctggc ggtcgtcgtc taccgcgtcg cctacgagca gaccggcaat
     2701 acgctcgcgg tctgggtcga cgcgagcgtc gaccgccact tcggtggctg gctgatgccc
     2761 gcgacctggg tgcagtcgct caacccgctg ttcgtgttcc tgctgacgcc gccgctcgtc
     2821 gcgctgtggc agcgacgcga actccggcgc ggcgcggcgc gcccgctcgc tcggatggcg
     2881 accggcgcgt tcgtctcggc gctggcgtgg ctgggcctgg cggcggtgat ccacagggac
     2941 ctcgcacagc acgcgcccac gggcatcgcc tgggtgacgg ccttcttcgc gctcttcacg
     3001 ctggcagagc tctacatcct gcccgtcggc ctcggcctgt tcgcgagcct cgcgccggcg
     3061 cgcttccggg cgaccacgat cgccgcctgg ttcctcgcgg cgttcgccgg caacctgctt
     3121 tccggcgtgc tcggcacggc gtggacgcac ctcgcgcccg acaccttctt cgtcctcacc
     3181 gccggcgtgg cggccctggc gggcgcgatg ctccgcgcga tcggcctgct ggccgaggcc
     3241 cggcgctgac ggcgcaagga tcgcgatggc cgaggatccg cgccacgccg gcccggcccc
     3301 aggccgtccg tacgacctcg agcagtcggc gctccagctc gccgcgctga tccgggcgag
     3361 cctggcggtc gggcacaccc gcgtcgccct gcagcgcagc ttcatgctgc tgtgcctgtg
     3421 cgggcgcctg ccggaagacc tcgcgccggt cgtcgaggac ggccttgccc gccggcccct
     3481 gcgcgagatc cgccgcatga agcaggccgc gcgcgcctgg gcggcgatgc tcgggacgcg
     3541 gggctgagcg cggcggatcc cgcgcgcacc gcgcgcccgc acccgttcag tagttgacgt
     3601 cccagcgcag caggccaccc atcgcggcga cccacaccac ccccagcaat gcgaacgcca
     3661 cgacgacggc ccacgcgcgc gtcaggcgcg aggcgtccga gcgcagcagc ttccacgcgg
     3721 cccaggcgcc ggcgacggcc gccgcgacgg ccagcaggcc gctcgcctgc agcaggcgga
     3781 cgatccagtc gttgccactg ttgtaggcgc cgatgtccgt gtcgaggacg ggcgcgatga
     3841 acgcggacca ggctgccagg tacagcgcgt ccacgacgat cgccgcgcgc tgcagccgcc
     3901 gcggcgtcgc cagacggggg ttgcccgcga ccggcgctcc gcccgcgcgg cgtgccagcg
     3961 cggccatcac ccaatacacc acgctcagca ccatcgtgag caacgagaac gcgagcacgc
     4021 ccagcgtcag cggcgccgag ctcgcgaacg gcgaggcctg cagcacgccg gtcgcgtcct
     4081 cgctgtcgac caccgtcttg acgccgccga cctccgcgag acgcagctgc cggctgccgc
     4141 ccagctcgcg ccacagcccg ggcgcgacct cgcgaaaggt ctgcgtgccc aggccgagcg
     4201 gatcgggggc gacgatcgtg ccgtccgcgt tcgcggtgat cacgacctgg ccgagcaggt
     4261 acagcaggct caggaatccg tgctcgatgc gccgggagcc ctggtagcgg ccggcgatcc
     4321 gaccggcgtc ggcccgcgcc gacgccagcg ccggcggatc gggcgccgac ggcgcgggga
     4381 agtagcgatc catgaagccg tcgaagagct cgcgccgggc ggcgtacacg gcgttgtcgc
     4441 gcccgcggct tttgaaggtg aagaagatgc ccacgccctc ctgcggcagc aggtcgagct
     4501 cggtgtggaa gacgatcgtg tcgcctccgt gcccgatcac cgtgcggccg ttctgcacgc
     4561 tgcggaagaa gccgtgcgcc atcgtcgcga agccgggcaa ggcgctctcg gtcggcgcgt
     4621 gcatcagcgc cgtcgtcttc gggtcgagga tggcgtggcc gtcgagccgg ccctgctgca
     4681 ggtgcgccag catgaagcgc gccatgtccg ccgcggtggt cgacgcgctg ccggccggcg
     4741 cggtgaccac gaactcgaac ggcgacggcg gccccgaggc cgtgcggtag ccctcggcca
     4801 gcgacggccg cagcggcgcg ggcacgggct gcgccatcgt gctgtgcgcc atgcccagcg
     4861 gctcgaacac gtggcgggcg atgtaggcgt cgtaggcctc gcccgagacg cgctgcacga
     4921 tgtagccggc gagcgcgacc ccgtagttgg aatacgccgg caccgtcccc ggcgggaaca
     4981 gcatcgcgcg cgggtgcgtc ttcaggtacc gctcggtcga ctgcgcccgc gccgggtcgt
     5041 aggacaggac gtccttcagg ccttcctcga agccgccgcg atggttcatc aggtcgcgca
     5101 tcgtcaccgg cttgccgtag ggctcgggca ccttgaagtc gaggtagtcg tcgacgtcgc
     5161 gatcgaggtc cagccggccc tgctgcacca gctgcatcac cgccgtccac gtgaacagct
     5221 tggacgtcga gcccgggcgc accagcgtcg tctgcggatc catcggccgc ctcgcagcga
     5281 cgtccgcata gccgtagccc ttctgcagca ggacgcgccc gtccttgacc accgagatca
     5341 ccatgccggc gatgtcgccc gtcttcagcg cgtatggcac gcggccgtcg agccaggcgg
     5401 acaggtccgc ggcatcgagg gcatgcgcgg cgtccggcgc ggacgcggac gcgccgggcg
     5461 ccggcgccgc ggccccgccc ggatccatcg ccgccgacgt accgcatgcc gccagcgccg
     5521 ccgcagccac cagggcggcc caggccgcga atcctcgtcg tcgcatgtcc attcccctcg
     5581 gcccgtcgcg ggcgcgtgat cggtcggctg cctgccgcct cccggattgt ccctttccgt
     5641 ccgggtgtcc ggcgaaatgt ttttcttatt gcgacatttt tgtacataac gtatatccat
     5701 caaaagccac agcgactcca ggaggattga gtgccccgga ggttttccga tccggatagt
     5761 cgacggcgac tctgctagag tgcccgcatg aatgcgaacg gccaccttcc cggcgacgcg
     5821 ggttcgcccg gagcgacgat ccgcgccctg cgcctcggca agggcctgac gctcgcgcag
     5881 ctcagcgagc gcaccggtct cgcgatctcg acgttgtcca aactggaaaa gggctcggtc
     5941 tcgctttcgt acgacaagct gatgctgatc agccgcggcc tgggcgtgga catggcgagc
     6001 ctggtcgacc cgaagccgcc ggtgccgcgg gcgcctgcgc tctcggcggg acgccgcgtc
     6061 gtgcagcgcg ccggcgaggg gcagctggtc gagacgcgca gctatcggca ggtctacctc
     6121 gccaccgagc tcctcaacaa gaagatgacg ccgatgttcg tggagatccg ggcccgcacg
     6181 atggaggagt tcacggcgga attcggcggc ctgatccgcc atcccggcga ggagttcacc
     6241 ttcgtgatcg agggcgagct ggacttccac agcgagctgt acgcgccgct gcggctgcgg
     6301 gccggcgact cgatctactt cgacagcgag atgggccatg cctacctcaa ggcgtccgag
     6361 gagccgtgcc ggctcgtctg cagttgcgcg ccgcgcggcg acgaggacac gatgaccgag
     6421 atgttcgtct cggcctcgga gcgcctggcc ccggccgccg cggaaccggc gcccagggca
     6481 cctcgggtgc agcgggcgcg caagcgcggc ggctgacgcc gtcgtcagcc ctgctggctc
     6541 gcgaacgtgc gtcggaactc cggcgcggca tcctcgcgcg tgttgacctc cagcatcagc
     6601 cgcccgggca tccagcagaa gaagcgcgac ccgcgcgcgt gcccgaagag atccgtctcc
     6661 atcggcacgc cggcatcgag catgtcggcg cgcatgcgct cgaggtccgc ccgggtcgcg
     6721 acctcgatgc cgaggtggaa gttgccgggc cacgcgacgt cccggtcgcg ggcgtcctcg
     6781 atgacgatgt cgagcgaccg tttcttgagc acgcggctcg tgcccatcgc gagcacctgg
     6841 aagccgagga accgctcgaa gaaggccgcc gtcgcgggga cgtcgtggga cgggaagctc
     6901 agatggttga gcgtggaagg aaccgtgtcg aagtgcgtcg tcatggggga acacctgtgc
     6961 gcaggccggc gcggcgcggg acgcgtcgcg atgtcggcgg gttgcgcgtt ggctgacgta
     7021 aacgcttacg gttcgatgca gaccgcgcgg gcggctgcac gacggggcgg atgataccgc
     7081 tttcctgtac cctcgcgctt tgccatccct ccgcatccgc cgcatgccct ggcacgcccg
     7141 cctcgacctg gactaccgca ccgatgccgc cggcgcctcc ggcaccccgc gcacccgcct
     7201 ggacttccgc cacgacggcc cgctgcgcat cctcaagagc ctgcatcccg aaggcgacgc
     7261 ggtctgccat tcggtgatcg tgcatccgcc cggcggcatc gtcggcggcg acacgctgga
     7321 cgtctcgatc gcgctggcgc cgggcgcgca cgcgctgctg acgacgcccg gcgcgacgcg
     7381 cttctaccgc agcgccggtg cgcgcgcggc ccaggccgtc gacgcgcgcc tggccgaggg
     7441 cgcgcgcctc gagtggctgc cgctcgagac cctggtgcac tcgggcgcgc tggccgacaa
     7501 ccggctgcgc ttcgacctcg cgccgggcgc cgagacgatg ggctg
//
LOCUS       DASVLN010000397         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_342239, whole genome shotgun sequence.
ACCESSION   DASVLN010000397 DASVLN010000000
VERSION     DASVLN010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342239"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VF453_09885"
     CDS             <1..469
                     /locus_tag="VF453_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688007.1"
                     /translation="AAGLVAGSSQAAGIDIHAGAEAADIGLPVYPGAVKRPEDDGSRD
                     DFSFGLWGSSFGVKIATVAYRSRDEVDDVSAFYRDALHRFGPVVDCSAGVPRAHAQPA
                     SGARTLDCGDDDADPGVRLYKSGSPDDQHVVEIKPLARGASFRLTRVQTRGLD"
     gene            complement(438..>2353)
                     /locus_tag="VF453_09890"
     CDS             complement(438..>2353)
                     /locus_tag="VF453_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX7688008.1"
                     /translation="MPAAAALFSPSADLTGGSASLLANTGRDPMFRGPALEQLVHAYV
                     GVDGDRAHALVSPALGEFHGFPPLLVHVGADEVLRDDGLRVAEKARAAGVVAESTVWP
                     VVPHAWQLLPQLPETRRSMDRAAEFLKTASAEAIEHLDSVVIGAGLSGIGAAVRLQHD
                     FPGRRFAILEARQAIGGTWDLFRYPGVRSDSDMYTLGYPFRPWTDARSLADGEAIRRY
                     IVDTAVEHGLERQVRFGQRVVRADWSSADACWTLEIEHAAADGAAPRRSRLRCDFLHA
                     CSGYYRYDAGHAPEFAGSAEFRGRIVHPQHWPQDLEVAGRRVVVVGSGATAVTLVPEL
                     AKSAAHVTMLQRSPSYVLSLPAVDRIAGALARVLPRAWAYRLARTKNIAVGMLFFQAS
                     RRWPAAVGRRLVAMVRTALGPGHDVETHFTPRYGPWDQRVCFVPDGDLFEAIRSGRAE
                     VATDTIERFTPSGIRLGSGRELDADVVVTATGLALNVLGDVAVTVDGVPQDLSKAFAY
                     KALMLGGVPNLVYTFGYTNASWTLKADLTARWTTRLLRHMQARGWRVAVPECPPDMAP
                     RPFLELKSGYVQRAAGILPRQGTKAPWTLAQNYLVDLIRLRLGRIADGTLRFARAGRE
                     DEALSRGRASARA"
BASE COUNT          318 a          866 c          889 g          280 t
ORIGIN      
        1 cgccgccggg ctcgtggcgg gcagctcgca ggccgccggc atcgacatcc acgccggtgc
       61 cgaggccgcc gacatcggcc tgccggtcta tcccggcgcg gtcaagcgcc ccgaggacga
      121 cggcagtcgc gacgacttct cgttcggcct gtggggcagc tccttcggcg tgaagatcgc
      181 gaccgtcgcc taccgcagcc gcgacgaggt cgacgacgtc tcggcgttct accgcgacgc
      241 gctgcaccgg ttcggcccgg tggtcgactg cagcgccggg gtgccgcgcg cgcacgcgca
      301 gccggcttcg ggtgccagga cgctcgattg cggcgacgac gatgcggatc cgggcgtgcg
      361 cctgtacaag tccggcagcc ccgacgacca gcacgtcgtc gagatcaagc cgctcgcgcg
      421 cggcgcgagc ttccggctca cgcgcgtgca gacgcgcggc ctcgactgag cgcctcgtcc
      481 tcgcgcccgg cgcgcgcgaa gcgcagcgtg ccgtccgcga tgcggcccag gcgcagccgg
      541 atcaggtcga ccaggtagtt ctgcgcgagc gtccacggcg ccttcgtgcc ctggcgcggc
      601 aggatgcccg cggcgcgctg cacgtagccc gacttgagct cgaggaaggg tcggggcgcc
      661 atgtccggcg ggcattcggg caccgcgacg cgccagccgc gcgcctgcat gtggcgcagc
      721 aggcgggtcg tccagcgcgc ggtcaggtcg gccttcagtg tccacgacgc gttcgtgtag
      781 ccgaaggtgt agacgaggtt gggcacgccg ccgagcatca gcgccttgta cgcgaaggcc
      841 ttggacaggt cctgcggcac gccgtccacc gtcacggcga cgtccccgag cacgttcagc
      901 gcgaggccgg tggcggtgac gacgacgtcg gcgtcgagct cgcggccgga gccgaggcgg
      961 atgcccgacg gggtgaagcg ctcgatcgtg tccgtcgcga cctccgcgcg gcccgagcgg
     1021 atcgcctcga acaggtcgcc gtcgggcacg aagcacacgc gctggtccca cgggccgtag
     1081 cgcggcgtga agtgggtctc gacgtcgtgc cccgggccga gcgccgtgcg caccatcgcg
     1141 accaggcgcc ggcccacggc cgcgggccag cggcgcgagg cctggaagaa cagcatgccg
     1201 acggcgatgt tcttcgtgcg cgcaagccgg taggcccagg cgcgcggcag cacgcgcgcg
     1261 agcgcgccgg cgatgcggtc gaccgcgggc aacgacagca cgtagctcgg cgagcgctgc
     1321 agcatcgtca cgtgcgccgc cgacttcgcg agctccggca ccagcgtcac cgcggtcgcg
     1381 ccgctgccga cgacgacgac gcgcctgccc gcgacctcca ggtcctgcgg ccagtgctgc
     1441 ggatgcacga tgcggccgcg gaactcggcg ctgccggcga actcgggcgc gtggccggcg
     1501 tcgtagcggt agtagccgct gcaggcgtgc aggaagtcgc agcgcaggcg cgagcgccgc
     1561 ggggccgcgc cgtcggcggc ggcgtgctcg atctccagcg tccagcacgc gtcggccgac
     1621 gaccagtccg cgcgcaccac gcgctggccg aagcgcacct ggcgctccag gccgtgctcg
     1681 acggcggtgt cgacgatgta gcggcggatg gcctcgccgt cggcgagcga tcgcgcgtcc
     1741 gtccacgggc ggaagggata ccccagggtg tacatgtccg agtccgagcg cacgcccggg
     1801 tagcggaaca ggtcccaggt gccgccgatc gcctggcgcg cctccaggat cgcgaaccgg
     1861 cggcccggga agtcgtgctg cagccgcacc gcggcgccga tgcccgacag cccggcgccg
     1921 atcacgacgg agtcgaggtg ctcgatcgcc tccgccgagg cggtcttcag gaattcggcc
     1981 gcgcggtcca tcgagcgccg cgtctccggc agctggggca gcagctgcca ggcgtgcggc
     2041 acgaccggcc agaccgtgct ctcggcgacc acgcccgcgg cgcgcgcctt ctccgcgacg
     2101 cgcaggccgt cgtctcgcag cacctcgtcg gcgcccacgt gcaccagcag cggcgggaag
     2161 ccgtggaact cgcccaacgc gggcgagacg agcgcgtgcg cgcgatcgcc gtcgacgccg
     2221 acgtaggcat gcaccagctg ctccagcgcg gggccgcgga acatcgggtc gcggccggtg
     2281 ttggccagca gcgaggcgct gccgccggtc aggtcggccg agggcgagaa cagcgccgcg
     2341 gccgcgggca tcg
//
LOCUS       DASVLN010000398         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_182080, whole genome shotgun sequence.
ACCESSION   DASVLN010000398 DASVLN010000000
VERSION     DASVLN010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182080"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VF453_09895"
     CDS             <1..913
                     /locus_tag="VF453_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7688009.1"
                     /translation="PTRILELDARDGVTRVLATSVAELPPADTISAPRAISYPSAGGR
                     VAHAFHYPPRNAGFVAPAGERPPLLVMIHGGPTAMATSTLRLAVQFWTSRGFAVLDVN
                     YGGSTGFGRAYRERLSGQWGVVDVEDCVAGARFLAAQGLVDAGRLAIRGGSAGGFTTL
                     AALAFHDVFKAGTSLYGVADLRALDDETHKFESRYLDDLLGPSAQREARYAERSPIHH
                     AHRIARPMLFLQGLDDKVVPPAQSEAMVAALRARGVPVAYLAFEGEGHGFRRKETVQR
                     ALEAELAFYAQVFGFAPADAIDPVTLA"
     gene            1003..1407
                     /locus_tag="VF453_09900"
     CDS             1003..1407
                     /locus_tag="VF453_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7688010.1"
                     /translation="MNDPSLFTVVAGSAEPIYRQLVAQVRRCVAGGQLKAGDELPSVR
                     ELAQALAVNPMTISKAFGLLEAEGLLTRRRGLSMVVAEQHARAQPAADRIELLRPTLE
                     RAAQEARQLQLPAAQAVKLFQTILEEQRENSP"
     gene            1404..>2164
                     /locus_tag="VF453_09905"
     CDS             1404..>2164
                     /locus_tag="VF453_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7688011.1"
                     /translation="MNTLATPTGGACFSLAGIRVAFDGKQVLADLDWALPAGTVVGLL
                     GRNGAGKTTLIETLLGLREPQAGRAFVFGQPALALDDAARARVGYVPQRSDLFEWMTA
                     DQLLAYFRSFYPRWNTAKVDALLSRWDIARDKPIGKLSGGQQQRLSIVRALAHDPELL
                     ILDEPVASLDPAARRDFLAEVVEQVVDRRTTVLFSTHILSDLERVAVDVAFLCGGRIA
                     LHAPLDELLDSSLRLGGAPADVQRFVAAHRLDLLTR"
BASE COUNT          272 a          811 c          777 g          304 t
ORIGIN      
        1 gccgacgcgc atcctcgagc tggacgcccg cgacggcgtg acgcgggtgc tcgcgacgag
       61 cgtcgccgag ctgcccccgg ccgacacgat ctcggcgccg cgcgcgatct cctatccgag
      121 cgccggcggg cgcgtcgcgc acgcgttcca ctatccgccg cgcaacgccg gcttcgtcgc
      181 gcccgcgggc gagcggccgc cgctgctcgt gatgatccac ggcggtccga cggccatggc
      241 cacgagcacg ctgcgcctgg ccgtgcagtt ctggaccagc cgcggcttcg cggtgctgga
      301 cgtcaactac ggcggctcga ccggcttcgg gcgcgcgtac cgcgagcggc tgtccgggca
      361 gtggggcgtg gtcgacgtgg aggactgcgt cgcgggcgca cgcttcctcg cggcgcaggg
      421 gctcgtcgac gccgggcgcc tcgcgatccg tggcggcagc gcgggcggct tcacgacgct
      481 ggcggcgctc gccttccacg acgtcttcaa ggcgggcacc agcctgtacg gcgtcgccga
      541 cctgcgtgcg ctcgacgacg agacccacaa gttcgaatcg cgctacctgg acgacctgct
      601 cggcccgtcc gcgcagcggg aggcgcgcta tgccgagcgg tcgccgatcc accacgcgca
      661 ccgcatcgcg cggccgatgc tgttcctcca gggcctggac gacaaggtcg tgccgccggc
      721 ccagtcggaa gccatggtcg cggcgctgcg cgcgcgcggc gtgccggtcg cctacctcgc
      781 cttcgagggc gagggccacg gctttcgccg caaggagacg gtgcagcggg cgctcgaggc
      841 cgagctcgcc ttctacgccc aggtgttcgg cttcgcgccg gccgatgcga tcgatcccgt
      901 gacgctggcc tgagaacctg gcgcggccgc cgggtcgagg gggttgcgga cagccgtctt
      961 ggtgtattac agtaacgata cacatagacg ccccgcatcc cgatgaacga tccgtcgctg
     1021 ttcaccgtcg tcgccggttc ggccgagccg atctaccgcc agctcgtcgc ccaggtgcgg
     1081 cgctgcgtgg ccggcgggca gctcaaggcc ggcgacgagc tgccgtcggt gcgcgagctc
     1141 gcgcaggcgc tggccgtcaa cccgatgacc atctccaagg ctttcggcct gctcgaggcc
     1201 gagggcctgc tcacgcgccg ccgcggcctg tccatggtgg tcgcggagca gcacgcccgg
     1261 gcccagcccg cggccgaccg catcgaactc ctgcgcccga cgctcgaacg cgcggcgcag
     1321 gaggcgcgac aactgcagct gcccgcggcc caggccgtga agctgttcca gaccatcctc
     1381 gaagagcaac gggagaactc gccatgaaca ccctcgccac gcccaccggc ggcgcctgct
     1441 tctcgctcgc gggcatccgc gtcgcgttcg acggcaagca ggtcctcgcg gacctcgact
     1501 gggccctgcc ggcgggcacc gtcgtgggcc tgctggggcg caacggcgcc ggcaagacga
     1561 cgctgatcga gacgctgctg ggcctgcgcg agccgcaggc gggccgcgcc ttcgtcttcg
     1621 gccagcccgc gctggcgctg gacgacgccg cgcgcgcccg ggtgggctac gtgccgcagc
     1681 gctcggacct gttcgagtgg atgacggccg atcagctgct ggcgtacttc cgcagcttct
     1741 acccgcgctg gaacacggcc aaggtggacg cgctgctctc gcgctgggac atcgcgcgcg
     1801 acaagccgat cggcaagctc tcgggcggcc agcagcagcg gctgtccatc gtgcgggcgc
     1861 tcgcccacga cccggagctg ctgatcctgg acgagccggt ggccagcctc gaccccgcgg
     1921 cccggcgcga cttcctcgcc gaggtggtgg agcaggtcgt cgaccgccgc acgacggtgc
     1981 tgttctccac gcacatcctg tcggacctgg aacgcgtggc cgtggacgtg gccttcctct
     2041 gcggcgggcg catcgcgctg cacgcgccgc tggacgagct gctcgactcg tcgctgcgcc
     2101 tgggcggcgc gccggcggac gtgcagcgct tcgtcgcggc gcaccgcctg gacctgctca
     2161 cgcg
//
LOCUS       DASVLN010000399         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_451400, whole genome shotgun sequence.
ACCESSION   DASVLN010000399 DASVLN010000000
VERSION     DASVLN010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451400"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VF453_09910"
     CDS             complement(<1..260)
                     /locus_tag="VF453_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7688012.1"
                     /translation="MRRPSAETLSDSRGVRLATSSRAAADHAEDALWQMMTLADTPRL
                     AIQAAREADPGWLLPVLLDAGFRLGLNQPADRAAARTLLAGA"
     gene            complement(274..1266)
                     /gene="argC"
                     /locus_tag="VF453_09915"
     CDS             complement(274..1266)
                     /gene="argC"
                     /locus_tag="VF453_09915"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7688013.1"
                     /translation="MSATATRFKVYVDGQEGTTGLRIHEMLAARPDIELLRIAPELRK
                     DDAERARLLNAADVAFLCLPDDAARAAARMVTNPRTCLIDASTAHRTDPSWVYGLPEL
                     APGQREALRAAKRIANPGCHATAFILLMRPLVDAGVVGADAPVSATSLTGYSGGGKKM
                     IEQYQAWHARQMDGERQVDAALSAPRPYALGLAHKHIPEMTACSKLARRPVFMPIVGN
                     FYKGLAVTIPFHLSELAAGTDAARVHAVLAERYADEQFVRVMPLNDAATLAEGQFDVQ
                     ACNDTQRADLFVFAGGDQLLLMARIDNLGKGASGAAVQTMNLHLGLPEGLGLER"
     gene            1379..1981
                     /locus_tag="VF453_09920"
     CDS             1379..1981
                     /locus_tag="VF453_09920"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7688014.1"
                     /translation="MTLHDPCRAALPAIHVLQLPGWQNSGDAHWQSRWEALHGDVRVE
                     QSDWMWPLRGDWMMRLDEAVLALEDAASAAGSQPPVIALSAHSLGCQLVAAWAAHSRH
                     TACVRAALMVAPPDTERADMPPNIHSFRPIVRTRLPFPARVVASSDDPFCAPGRARAM
                     AADWGAEFVDAGPRGHVNADSGLGDWPEGRTLLAALLARP"
     gene            2035..2961
                     /locus_tag="VF453_09925"
     CDS             2035..2961
                     /locus_tag="VF453_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7688015.1"
                     /translation="MVTKKPKGLGMGLEALLGPKHDDVAPSADGPVQPATLKLGQMQA
                     GRYQPRTRMDEGSLYELAESIRSQGIMQPILVRPIEGAGAKAQRYEIIAGERRFRAAR
                     LAGLEEVPVLVREVPDESAAVMALIENIQREDLNPLEEAQGLKRLVDEFGLTHEQAAQ
                     AVGRSRSAASNLLRLLNLAAPVQQMLMAGDIDMGHARALLALEPAQQILNANEIAAKK
                     LSVRDAEKLVARSQASARAAAGPAGTKPRKEKSGDIARLEEQLADKLAATVEIRLLKK
                     TRHGESGEVAISFASLDELNGLLERLGLGEEA"
     gene            complement(3025..>3419)
                     /locus_tag="VF453_09930"
     CDS             complement(3025..>3419)
                     /locus_tag="VF453_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688016.1"
                     /translation="SGDGTSRRSAGSQAVGLVRAAASHWDGVAAPVALLSCTASSSTA
                     GHCSAGPLVQLDLGTGASLTLLSLASTTPLVLRGDATQGLETAFGAETLGGGGGVGFN
                     VNDVDARDALQFSPGGPGLASRVTTYIP"
BASE COUNT          459 a         1233 c         1304 g          423 t
ORIGIN      
        1 tgcgcgcccg cgagcagcgt gcgcgccgcc gcgcggtcgg caggctggtt gaggccgagc
       61 cggaagcccg cgtcgagcag caccggcagc agccagcccg ggtcggcctc gcgcgcggcc
      121 tggatcgcca ggcgcggcgt gtccgcgagc gtcatcatct gccagagcgc gtcctcggca
      181 tggtcggccg cggcgcgcga ggacgtggcc aggcgcacgc cgcgcgagtc ggacagcgtc
      241 tcggcggacg ggcgcctcat gcgtagcgcg ccttcagcgc tccaggccga gcccttcggg
      301 caggccgagg tgcaggttca tcgtctgcac cgcggcgccc gaggcgccct tgccgaggtt
      361 gtcgatgcgc gccatcagca gcagctggtc gccgccggcg aagacgaaca ggtcggcgcg
      421 ctgcgtgtcg ttgcaggcct ggacgtcgaa ctggccctcg gcgagcgtcg cggcgtcgtt
      481 cagcggcatc acgcggacga actgctcgtc ggcgtagcgc tcggccagca cggcgtgcac
      541 gcgcgccgcg tccgtgcccg ccgccagctc ggacaaatgg aacgggatcg tgacggccag
      601 gcccttgtag aagttgccca cgatcggcat gaacaccggc cggcgcgcga gcttcgagca
      661 ggcggtcatc tccgggatgt gcttgtgcgc cagccccagg gcgtacggcc gcggcgcgct
      721 gagcgcggcg tcgacctggc gctcgccgtc catctggcgc gcgtgccagg cctggtactg
      781 ctcgatcatc ttcttgccgc cgccggagta gccggtcagc gacgtcgcgg agaccggcgc
      841 gtccgcgccg acgacgcccg cgtccacgag cggccgcatc agcaggatga acgcggtcgc
      901 gtggcagccc gggttggcga tgcgcttggc ggcgcgcaac gcctcgcgct ggcccggcgc
      961 gagctcgggc aggccgtaga cccacgacgg gtcggtgcgg tgggcggtgc tggcgtcgat
     1021 caggcaggtc cgcgggttcg tgaccatcct cgcggcggcg cgggcggcgt cgtcgggcag
     1081 gcacaggaac gcgacgtcgg ccgcgttgag caggcgcgcg cgctcggcgt cgtccttgcg
     1141 cagctcgggg gcgatgcgca gcagctcgat gtcggggcgg gcggcgagca tctcgtggat
     1201 gcgcaggccg gtcgtgcctt cctggccgtc gacgtagacc ttgaatcggg tggcggtggc
     1261 gctcatggcg gcaagctttc ctgcgggctg gcgcgccgcc gccgggctgg gcggtcgacg
     1321 cggcagaatc tcgggggacc ggccgctggg gccggacgaa tccgccaagc atacggcaat
     1381 gacattacac gacccctgcc gggccgcgct gcccgcgatc cacgtgctgc agctgcccgg
     1441 atggcagaat tcgggcgacg cgcactggca gagccgctgg gaggcgctgc acggcgacgt
     1501 gcgcgtggag cagtcggact ggatgtggcc gctgcgcggc gactggatga tgcggctcga
     1561 cgaggcggtc ctcgcgctcg aggacgccgc ctcggccgcc ggctcgcagc cgccggtgat
     1621 cgcgctgtcc gcgcacagcc tgggctgcca gctcgtggcc gcgtgggccg cgcactcgcg
     1681 ccacacggcc tgcgtgcgcg cggccctgat ggtggcgccg ccggacaccg agcgcgccga
     1741 catgccgccc aatatccact cgttccgccc gatcgtgcgc acgcggctgc cgttcccggc
     1801 gcgcgtcgtc gccagctcgg acgacccgtt ctgcgcgccc ggacgcgcgc gggcgatggc
     1861 cgcggactgg ggcgccgagt tcgtcgacgc aggccctcgc gggcacgtca acgccgattc
     1921 cgggctcggc gactggcccg agggccggac cctgctcgcg gcgctgctgg cgcgcccata
     1981 atccgtcctt tcgcgcacgc gccgcggcgc actgccaacg tcgagggcac acacatggtc
     2041 acgaagaaac cgaagggtct gggcatgggc ctcgaggccc tgctgggccc gaagcacgac
     2101 gacgtcgcgc cgtccgccga cggcccggtg cagcccgcga cgctcaagct cggccagatg
     2161 caggccggcc gctaccagcc gcgcacgcgc atggacgagg gctcgctcta cgagctggcc
     2221 gaaagcatcc gcagccaggg catcatgcag ccgatcctcg tgcggccgat cgagggcgcg
     2281 ggcgcgaagg cgcagcgcta cgagatcatc gccggcgagc gccgcttccg cgccgcgcgg
     2341 ctggcgggac tcgaggaggt gccggtgctc gtgcgcgagg tgcccgacga gtccgccgcc
     2401 gtgatggcgc tgatcgagaa catccagcgc gaggacctga acccgctcga agaggcgcag
     2461 ggcctcaagc gcctggtcga cgagttcggc ctgacccacg agcaggccgc gcaggcggtg
     2521 ggccgctcgc gcagcgccgc gagcaacctg ttgcgcctgc tcaacctcgc cgcgccggtg
     2581 cagcagatgc tgatggccgg cgacatcgac atgggccacg cgcgcgcgct gctggcgctg
     2641 gagccggcgc agcagatcct gaacgccaac gagatcgccg cgaagaagct ctcggtgcgc
     2701 gacgcggaga agctggtcgc gcgcagccag gcctcggcgc gcgccgccgc cggcccggcc
     2761 ggcacgaagc cgcgcaagga gaagtccggc gacatcgcgc gcctcgagga gcagctggcc
     2821 gacaagctgg ccgcgacggt cgagatccgc ctgctgaaga agacgcgcca cggcgaatcg
     2881 ggcgaggtcg cgatctcgtt cgcctcgctc gacgagctca acgggctgct cgagcgcctg
     2941 ggcctcggcg aagaggcgtg actgcccggc ctggtacggc agggggtatc ccgtaccgcg
     3001 ccgcggcaag cggcccggcg cccgtcaagg gatgtacgtc gtcacgcggc tggccagtcc
     3061 cgggccgccg ggcgagaact gcagggcgtc gcgcgcgtcg acgtcgttga cgttgaagcc
     3121 gacgcctccg ccgccgccca gggtctcggc gccgaaggcg gtctccaggc cctgcgtcgc
     3181 gtcgccgcgc agcaccaggg gcgtggtcga ggcgagcgag agcagcgtga gcgacgcgcc
     3241 cgtgccgagg tcgagctgga ccagcggccc ggccgagcag tggcccgccg tcgagctgga
     3301 cgcggtgcac gacagcagcg ccacgggcgc ggcgacgccg tcccaatgcg acgccgcggc
     3361 gcgcaccagg ccgaccgcct ggctgcccgc gctgcgccgg ctcgtgccgt cgccgctga
//
LOCUS       DASVLN010000400         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_234853, whole genome shotgun sequence.
ACCESSION   DASVLN010000400 DASVLN010000000
VERSION     DASVLN010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234853"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VF453_09935"
     CDS             <1..603
                     /locus_tag="VF453_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX7688017.1"
                     /translation="VGKAAPAAGHLVSDRALAAMEVTRRGAAYRESQVVRWSHYDNAI
                     LRSAGRPAGNSRIWGDAPPATQAAAIDALVRASRAAGLGNHDTALVLAIAHTESGFNP
                     DAAAGTTSASGLGQFVDATAQHYGLGNANRWDVDAQAKALVAHFVDNRNLAHARHQGD
                     EYIYKYHHDGPSKDYGGLAISERKVMPLAERYEALLNASR"
     gene            608..1099
                     /locus_tag="VF453_09940"
     CDS             608..1099
                     /locus_tag="VF453_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688018.1"
                     /translation="MKTAAPAACLVLAIAARAVVAAAPAASAVDTAPRTAATAACGGA
                     EFRVEVTHASAHPLDNTYRLYGGPHGMPGQLLYTTDVGGWFHAACVKDGAGRPRLLFQ
                     SSCGGSGCPEDRFGIVDPSTLALLLRPPTGRRGNIKDADRLLRIRTPWIANDGSAFCC
                     DAR"
     gene            complement(1201..2094)
                     /locus_tag="VF453_09945"
     CDS             complement(1201..2094)
                     /locus_tag="VF453_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1521 domain-containing protein"
                     /protein_id="HEX7688019.1"
                     /translation="MTITTTATPAGTAAASSNVLLSSPITNPSHASTTMQNGQAVFEN
                     DNYKITADDNNTVTITNKHTGAVYQASGDPHVSVNGQHKFDFWGTTTFNLDDGTKVTI
                     QTTPYAANPNADLSSKVTITNGSYGVQITGVDSNMKGDLAIHEAAGWGNTLDWAIDDG
                     NEIYESAAGNNLVGLDAAGNEHAVDQNWINATDILKNPNANPNAAPVASSPNQQLGAA
                     YRDAFRMLDSLVSIVFRGAFSGNVGASGSGHAHPDHGGYHDVEPQRPRPIFEPWIGGG
                     LIAHADIDVNVNVNVSLRLGR"
BASE COUNT          307 a          811 c          849 g          372 t
ORIGIN      
        1 gtcggcaagg cggccccggc ggccggtcat ctcgtgagcg accgggcgct cgccgcgatg
       61 gaggtgacca ggcgcggcgc ggcctaccgc gagtcccagg tcgtgcgctg gtcgcactac
      121 gacaacgcga tcttgcgcag cgccggccgg cccgcgggga actcgcgcat ctggggcgat
      181 gcaccgcccg cgacgcaggc ggcggcgatc gacgcgctgg tgcgcgcctc gcgcgcggcc
      241 gggctcggca accacgacac cgcgctggtg ctggcgatcg cgcacacgga gtcgggcttc
      301 aaccccgacg cggccgcggg cacgacgtcg gcgagcggcc tgggccagtt cgtcgacgcc
      361 accgcccagc actacggcct cggcaatgcc aaccgctggg acgtggacgc gcaggcgaag
      421 gcgctggtgg cgcacttcgt cgacaatcgc aacctcgcgc acgcccgcca ccagggcgac
      481 gagtacatct acaagtacca tcacgacggc ccgagcaagg actacggcgg cctggccatc
      541 tccgagagga aggtcatgcc gctcgccgag cgttacgaag cgctgctgaa cgccagccgt
      601 tgaccgcatg aagactgccg cccccgccgc ctgcctcgtc ctcgcgatcg ccgcccgcgc
      661 cgtcgtggcc gccgccccgg cggcgtcggc ggtggatacg gcaccccgta ccgccgcgac
      721 cgcagcctgc ggcggcgctg agttccgggt cgaggtcacg cacgcgagcg cgcatccgct
      781 ggacaacacc taccgcctct acggcggccc gcatggcatg cccgggcagc tgctgtacac
      841 gaccgacgtc ggcggctggt tccacgccgc ctgcgtgaag gacggggcgg ggcggccgcg
      901 gctgctgttc cagtcctcgt gcggcggctc cggctgcccg gaggatcgct tcggcatcgt
      961 cgacccgtcg accctcgcgc tgctgctgcg cccgccgacc ggacgccgcg gcaacatcaa
     1021 ggacgccgat cgcctgctgc gcatccgcac gccgtggatc gccaacgacg gctcggcgtt
     1081 ctgctgcgac gcgcgctgac gagcgccgcg ccgctcccat gagcgaggac gccgccgatc
     1141 tcccgaccgg cggcgccagg gacgggcccg gagacagtcg ggcccgcgcg gatcgtcgcc
     1201 tcagcgcccc aggcgcagcg agacgttgac gttcacgttg acgtcgatgt cggcgtgggc
     1261 gatcaggccg ccgccgatcc acggctcgaa gatcgggcgc ggacgctggg gctcgacgtc
     1321 gtggtagccg ccgtggtcgg gatgcgcatg gccgctgccc gaggcgccca cgttgccgct
     1381 gaacgcgccg cggaagacga tgctgaccag gctgtcgagc atgcggaacg cgtcgcggta
     1441 ggcagcgccg agctgctggt tgggcgagct cgcgaccggc gcggcgttgg ggttcgcgtt
     1501 cgggttcttg aggatgtccg tcgcgttgat ccagttctgg tcgaccgcgt gctcgttgcc
     1561 cgcggcgtcc aggcccacca ggttgttgcc ggcggcgctc tcgtagatct cgttgccgtc
     1621 gtcgatcgcc cagtccagcg tgttgcccca gccggccgcc tcgtggatcg ccaggtcgcc
     1681 cttcatgttc gagtcgacgc cggtgatctg cacgccgtag ctgccattgg tgatggtcac
     1741 cttggacgac aggtcggcgt tcgggttcgc ggcgtagggc gtcgtctgga tcgtcacctt
     1801 ggtgccgtcg tccaggttga aggtcgtggt gccccagaag tcgaacttgt gctggccgtt
     1861 caccgacacg tgcggatcgc cggaggcctg gtagaccgcg cccgtgtgct tgttggtgat
     1921 cgtgacggtg ttgttgtcgt cggccgtgat cttgtagttg tcgttctcga acaccgcctg
     1981 gccgttctgc atcgtcgtgc tggcgtggct gggattggtg atcggcgacg acagcaggac
     2041 gttgctcgac gcagcggcgg taccggcggg ggtggccgtg gtggtgatgg tcatcctctc
     2101 tactccctga gctgtttgga tcggcctgca ccctttgcgg gccgatgggg acgtattgaa
     2161 gaggttcctg catcgattcg taagctgggt cggttcccag ctgggaatcg acctaggcgc
     2221 cgggcgcgtc gacgcgcagc acccagcccg cctgcagcgg cgcgctggcg aaggcgggag
     2281 cgcccgcggg tgcgccggga aggcgcaggc cgatcggcat caggtgcccg tgcagcgcc
//
LOCUS       DASVLN010000401         1800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_234893, whole genome shotgun sequence.
ACCESSION   DASVLN010000401 DASVLN010000000
VERSION     DASVLN010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234893"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..>1800)
                     /locus_tag="VF453_09950"
     CDS             complement(67..>1800)
                     /locus_tag="VF453_09950"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HEX7688020.1"
                     /translation="LGEQEAGAGLAALERRARDAELLIQLNRYGEVLERVGDAQEARR
                     RLRESLLLESELGRRDRRDTVVALQRQSSFEQHQRHMEQLVHENALQSAEIARRNNER
                     TLMLLLALAMAVGGGAAGRLWLGARRSNRQLAVNNAELAFASLHDKITGLYNRRAMEA
                     DTEALELAGGRRFCVVTISVKQFGLIVGSVGHQLGDTLLCQIAARLEAAAARFDGRLY
                     RVDGVTFGALFEFGADAPRLQAILEALAGAMEAPFSIGNQDLIVSIGAGASEYPKDAG
                     TAQEVARLAELAKLEAHADPGNSFLVYDSRLGESQRDKLRMESRMLKALEAGHFELFY
                     QGQRGLPDGRICGFEALLRWRDGDAMIPPAQFIPLAEETGLIVRIGAWVLEQACRQAK
                     AWADSGAGRPLVAVNISPRQFNHPDFLGVVRDTLARTGVDPRQIELEITEGSVMNDAE
                     ASIAQLHALRALGLHLAIDDFGTGYASLSYLRRFPLDRLKIDRAFIRQLNASEQDDTI
                     VRTVIELAHALGLSVTAEGVETIEQEDRLQHWGCDVVQGFLRFRPAPAATATALLQQD
                     TAAAARAPEPA"
BASE COUNT          228 a          650 c          666 g          256 t
ORIGIN      
        1 cgctggaggc cgagtcgtgc tgagttcggg ggcaccggcg aggcgcggac gccggcgcat
       61 gcatgctcag gccggctcgg gggcgcgcgc ggccgccgcc gtgtcctgct gcagcagggc
      121 ggtggccgtc gcggcgggcg cggggcggaa gcgcaggaag ccctggacga cgtcgcagcc
      181 ccagtgctgc aggcgatcct cctgctcgat cgtctccacg ccctcggccg tcacggacag
      241 gcccagcgcg tgcgcgagct cgatcacggt gcgcacgatg gtgtcgtcct gctcgctcgc
      301 gttgagctgg cggatgaacg cgcggtcgat cttgagccgg tccagcggaa accggcgcag
      361 gtaggacagg ctcgcgtagc cggtgccgaa gtcgtcgatc gccaggtgca ggcccagcgc
      421 gcgcagcgcg tgcagctgcg cgatgctcgc ctcggcgtcg ttcatcacgc tgccctcggt
      481 gatctcgagc tcgatctggc gcggatcgac gcccgtacgg gccagggtat cgcgcaccac
      541 gccgaggaag tcgggatggt tgaactggcg cggcgagatg ttgaccgcca ccagcggccg
      601 ccccgcgccc gagtccgccc atgccttggc ctggcggcag gcctgttcga gcacccaggc
      661 gccgatgcgc acgatcaggc cggtctcctc ggcgagcggg atgaactgcg cgggcgggat
      721 catggcgtcg ccgtcgcgcc agcgcagcag cgcctcgaag ccgcagatgc ggccgtcggg
      781 caggccgcgc tggccctggt agaacagctc gaagtggccc gcctccagcg ccttgagcat
      841 gcgactctcc atgcgcagct tgtcgcgctg gctctcgccg aggcggctgt cgtacacgag
      901 gaagctgttg cccgggtcgg cgtgcgcctc gagcttcgcg agctccgcca ggcgcgcgac
      961 ctcctgcgcg gtgccggcgt ccttcgggta ctcgctcgcg ccggcgccga tcgagacgat
     1021 cagatcctgg ttgccgatgc tgaacggcgc ctccatcgcg cccgcgagcg cctcgaggat
     1081 cgcctgcagc cgcggcgcgt cggcgccgaa ctcgaacagg gcgccgaagg tgacgccgtc
     1141 gacgcggtag agccggccgt cgaagcgcgc ggccgccgcc tccaggcgcg cggcgatctg
     1201 gcacagcagc gtgtcgccca gctggtggcc gacgctgccg acgatcaggc cgaactgctt
     1261 gaccgagatc gtgacgacgc agaagcggcg gccgccggcg agctccagcg cctcggtgtc
     1321 ggcctccatc gcgcggcggt tgtacaggcc cgtgatcttg tcgtgcaggc tcgcgaacgc
     1381 gagctccgcg ttgttgacgg cgagctgccg gttcgagcgc cgtgcgccca gccacagccg
     1441 gcccgccgcg ccgccgccga cggccatcgc gagcgccagc agcagcatca gcgtgcgctc
     1501 gttgttgcgg cgcgcgatct cggcggactg cagcgcgttc tcgtgcacca gctgctccat
     1561 gtggcgctgg tgctgctcga agctggactg gcgctgcagc gcgacgaccg tgtcgcggcg
     1621 gtcgcggcgg ccgagctcgc tttccagcag cagcgactcg cgcaggcgcc gccgcgcctc
     1681 ctgggcgtcg ccgacgcgtt cgagcacctc gccgtagcga ttgagctgga tcagcagctc
     1741 ggcgtcgcgc gcgcggcgct ccagcgcggc caggccggcg ccggcctcct gctcgcccag
//
LOCUS       DASVLN010000402         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_290168, whole genome shotgun sequence.
ACCESSION   DASVLN010000402 DASVLN010000000
VERSION     DASVLN010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290168"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VF453_09955"
     CDS             complement(<1..300)
                     /locus_tag="VF453_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX7688021.1"
                     /translation="MIPLAFPRIAIVGGGPGGLTLARLLHVRGIAATVFEREADPDAR
                     AQGGTLDLHQDSGLLALAEAGLAHGLAAIARPEDQGVRVLHPDGGVLLDHDGAGGD"
     gene            364..807
                     /locus_tag="VF453_09960"
     CDS             364..807
                     /locus_tag="VF453_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7688022.1"
                     /translation="MDDVDDPNPMRLMGAIHRSFVRVVDGPLRELGFAVGQLVVLVTL
                     RRAGPQPQSQLVRAAKVEQSSMAQLLARMERDGLVERVPDPQDGRSRLVRLTDGAARR
                     LPRAKALMDDTSERALAGFSEKERAQLLGFLRRVHANLEAVEPRE"
     gene            828..1229
                     /locus_tag="VF453_09965"
     CDS             828..1229
                     /locus_tag="VF453_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEX7688023.1"
                     /translation="MDYLLVRGLHETCVLASGTGFAARFALGLAGRPRPRSRLTRALP
                     HALDTLLLASALTLLALAHLNPLATPWLRAKLVGLLLYIAFGYVALRPDVPRLVRAVA
                     GFAALETFAWIVSVAFTKQPLGFLAVLVAGG"
     gene            complement(1249..>1639)
                     /locus_tag="VF453_09970"
     CDS             complement(1249..>1639)
                     /locus_tag="VF453_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-beta hydroxysteroid dehydrogenase"
                     /protein_id="HEX7688024.1"
                     /translation="ELARRKGEAAFIGEGLNRWPGVHRLDAARLYRLALEKPQRGARW
                     HAVDEEGVPFRDIAAAIGRGLGVPVVSKSGDEVAAHFGWFSVFAAMDAPASNARTRAA
                     LGWAPTRPGLLEDLALPAYFVPGQRVL"
BASE COUNT          211 a          616 c          595 g          217 t
ORIGIN      
        1 atcgccgccg gcgccgtcgt gatcgagcag cacgccgccg tccggatgga ggacgcgcac
       61 gccctggtcc tcgggccgcg cgatcgcggc caggccgtgg gccagcccgg cctcggcgag
      121 cgcgagcagg cccgagtcct ggtgcaggtc cagcgtgccg ccctgcgcgc gtgcgtcggg
      181 atcggcttca cgctcgaaga cggtggcggc gatgccgcgc acgtgcagca gccgcgccag
      241 cgtgaggccg ccggggccgc cgccgacgat ggcgatgcga gggaaagcga gagggatcat
      301 ggcgaggcat gcgtcctaga atgataggag cctctataat tacataggcc cctatcaaaa
      361 tcaatggacg acgtcgacga tcccaacccg atgcgcctga tgggcgcgat ccaccgcagc
      421 ttcgtgcgcg tggtcgacgg gcccttgcgc gagctcggct tcgccgtggg ccagctggtg
      481 gtgctggtga cgctgcgccg cgccggcccg cagccgcagt cgcagctggt gcgggcggcg
      541 aaggtcgagc agtccagcat ggcgcagctg ctcgcgcgca tggagcgcga cggcctggtc
      601 gagcgcgtgc ccgacccgca ggatggccgc agccgactcg tgcgcctgac cgacggcgcc
      661 gcgcgccgcc tgccgcgcgc caaggcgctg atggacgaca cgagcgagcg cgcgctcgcc
      721 ggcttcagcg agaaggaacg cgcgcagctg ctcggcttcc tgcggcgggt gcatgccaac
      781 ctcgaggccg tcgaaccgcg ggagtaggat tcccgcggac tgccgacatg gactacctgc
      841 tcgtgcgcgg cctccacgag acctgcgtgc tcgcctcggg caccggcttc gcggcgcgtt
      901 tcgccctcgg actggcgggt cggccgcggc cgcgctcgcg gctgacgcgc gcgctgccgc
      961 acgcgctcga cacgctgctg ctggcatcgg cgctcacgct gctcgcgctc gcgcacctga
     1021 accccctggc gacgccctgg ctgcgcgcca agctcgtcgg cctgctgctc tacatcgcgt
     1081 tcggctacgt cgccctgcgg cccgacgtgc cgcgcttggt gcgcgcggtc gccggcttcg
     1141 cggcgctgga gaccttcgcg tggatcgtct ccgtcgcgtt cacgaagcag ccgctcggct
     1201 tcctcgcggt gctggtggcc ggaggctgag cggccgccga gaccctcttc aaagcacgcg
     1261 ctggcccggc acgaaatagg cgggcagcgc caggtcctcc agcagccctg gccgcgtcgg
     1321 cgcccagccc agcgccgcgc gcgtgcgcgc gttcgaggcg ggcgcgtcca tcgccgcgaa
     1381 gacggagaac cagccgaagt gcgcggcgac ctcgtcgccc gacttcgaga ccaccggcac
     1441 gcccagcccg cgcccgatcg cggcggcgat gtcgcggaac ggcacgccct cctcgtcgac
     1501 cgcgtgccag cgcgcgccgc gctgcggctt ctcgagcgcc agccggtaca gccgcgccgc
     1561 atccaggcgg tgcacgccgg gccagcggtt gaggccctcg ccgatgaacg ccgcctcgcc
     1621 cttgcggcgc gcgagctcg
//
LOCUS       DASVLN010000403         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_128678, whole genome shotgun sequence.
ACCESSION   DASVLN010000403 DASVLN010000000
VERSION     DASVLN010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128678"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VF453_09975"
     CDS             <1..997
                     /locus_tag="VF453_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX7688025.1"
                     /translation="LSQGVGPEALAAAIGRPAEALASTRDSLSVDDYLALLEAGARLA
                     RDPAFGLHVGLQARLSTYPFYGLVVCACKTLREAFAQTRRYEGLAHDLGRSRLEERDG
                     LATYFWDCPWLAAATPAQARHLCDSVLAGILTFVAWLAHERLPVREIGLPTPEPEPAL
                     RAAHEQVFGTRIAYGVPVAYGRFDAALLDRPVPNADASMFEVLRRHADELLAARLRET
                     AEPRVVVDVRREVAAQLANDRARLDDVAAALGLTPRTLQRKLADAGTSFQAVHDAARQ
                     ALAEELLRDSRLNLTDVAYLLGYREQSSFNRACRDWFGNTPARTRERLRDASSLR"
     gene            complement(1019..1681)
                     /locus_tag="VF453_09980"
     CDS             complement(1019..1681)
                     /locus_tag="VF453_09980"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX7688026.1"
                     /translation="MTSATSTATTAPEGLFHDDDRVLRCQWCRASAEYRRYHDEEWGF
                     PVTDERRLFEKICLEGFQAGLSWLTILNKREAFRKGFANFDAARVARFDERDVERLLA
                     DAGIVRHRGKIESTINNARRVLELREEFGSLAAYAWGFEPEPSTRPPRLLPGTAAGPS
                     AEAVAMSKDLKKRGWTFVGPTTVYAFMQAMGLVNDHYESCHAREKALAAREALDRPGR
                     RG"
     gene            1867..2343
                     /locus_tag="VF453_09985"
     CDS             1867..2343
                     /locus_tag="VF453_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7688027.1"
                     /translation="MLRKSLTVMSLLAAFAGAAFAAERPYDESADAKSEIRTALKEAA
                     AAKQPVLIIFGANWCEDCRALDHALRTGRNAELISRDFRVVKVDVGNFDRNLDVTGAY
                     GNPTAHGIPSAVIVSPDNRILFATRAGELADARRMSEDGIHDFFAKAAGTVGAGAH"
     gene            complement(2354..>2774)
                     /locus_tag="VF453_09990"
     CDS             complement(2354..>2774)
                     /locus_tag="VF453_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX7688028.1"
                     /translation="DAWRSGLTPLEKAATLEALAAGGAKVLMVGDGLNDAPALAAADV
                     GIAMAGGTDVAQHAAGITLMRNDPRLVADALAISRRCVAKIRQNLFWAFVYNVAGIGL
                     AAAGLLSPMVAGAAMAFSSVSVVLNALALRRWRPGAD"
BASE COUNT          354 a         1059 c          981 g          380 t
ORIGIN      
        1 gctgtcgcag ggcgtcggcc cggaagcgct cgccgccgcg atcggccgcc ctgccgaggc
       61 gctggcgtcc acgcgcgaca gcctgtcggt ggacgactac ctcgcgctgc tcgaggccgg
      121 tgcccgcctg gcccgcgatc cggccttcgg cctgcacgtc gggctgcagg cgcggctgtc
      181 gacctatccg ttctacggcc tcgtcgtctg cgcgtgcaag acgctgcgcg aagcgttcgc
      241 ccagacgcgc cgctacgagg ggctcgcgca cgacctcggc cgctcgcggc tggaggagcg
      301 cgacggcctc gcgacctact tctgggactg cccctggctc gccgcggcca ccccggcgca
      361 ggcgcgccac ctgtgcgaca gcgtgctcgc cggcatcctg accttcgtcg cctggctcgc
      421 gcacgagcgc ctgccggtgc gcgagatcgg cctgccgacc ccggagcccg agcccgcgct
      481 gcgcgccgcc cacgagcagg tcttcggcac gcgcatcgcc tatggggtgc cggtcgcgta
      541 cggccgcttc gacgccgcgc tgctcgaccg ccccgtgccc aacgccgacg ccagcatgtt
      601 cgaggtgctg cgccgccacg ccgacgagct gctcgccgcg cgcctgcgcg agacggccga
      661 gccgcgggtc gtcgtcgacg tccggcgcga ggtcgccgcc cagctcgcca acgaccgcgc
      721 ccgcctcgac gacgtcgccg cggcgctcgg tctcaccccg cgcaccctgc agcgcaagct
      781 cgccgacgcc ggcaccagct tccaggcggt gcacgacgcc gcccgccagg cgctggccga
      841 ggaactgctg cgcgacagcc gcctcaacct caccgacgtc gcctacctgc tcggctaccg
      901 cgagcagagc tccttcaacc gtgcctgccg cgactggttc ggcaacacgc cggcacgcac
      961 gcgggaacgc ctgcgggacg cgtcctcgct tcgctgagcg gccggcgccc gcctcgcgtc
     1021 agccgcggcg cccgggtctg tccagcgcct cgcgtgccgc cagcgccttc tcgcgcgcat
     1081 ggcaggactc gtagtggtcg ttgaccaggc ccatcgcctg catgaaggcg tagacggtgg
     1141 tgggcccgac gaaggtccag ccgcgcttct tcaggtcctt ggacatcgcg accgcctccg
     1201 ccgacggccc cgcggcagtg ccgggcaaca ggcgcggcgg gcgcgtggac ggctcgggct
     1261 cgaagcccca ggcgtaggcc gcgagggagc cgaactcctc gcgcagctcc aggacgcgcc
     1321 gggcgttgtt gatcgtcgac tcgatcttgc cgcgatggcg cacgatgccg gcgtcggcga
     1381 gcaggcgctc gacgtcgcgc tcgtcgaagc gcgcgacccg ggcggcgtcg aagttggcga
     1441 agcccttgcg gaaggcctcg cgcttgttga ggatggtgag ccagctgagg ccggcctgga
     1501 agccttccag gcagatcttc tcgaacaggc ggcgctcgtc cgtgaccggg aagccccatt
     1561 cctcgtcgtg gtagcggcgg tattcggcgc tcgcgcgaca ccactggcag cgcagcacgc
     1621 ggtcgtcgtc gtggaacagg ccctcggggg cggtggtggc ggtggaggtc gcggaggtca
     1681 ttggatgatt ctgccgtcgc gcggccgcgg ccgtgcgcgt cgggtcgggg gatttccggg
     1741 ctggaatgcg ccgcgcgcgc gtcaggatcg cccgaaaccc acgttgcccg cgatggccgt
     1801 gccgtcgcga gatccccgcg atgctggcat gatgtccatc gcttccctag acaacgtcgc
     1861 gccgccatgc tccgcaagtc cctgaccgtg atgtccctgc tcgccgcgtt cgccggcgcc
     1921 gcgttcgccg ccgagcggcc gtacgacgag tccgcggacg ccaagtccga gatccgcacg
     1981 gcgctcaagg aggccgcggc ggcgaagcag ccggtgctga tcatcttcgg cgccaactgg
     2041 tgcgaggact gccgcgcgct cgaccacgcg ctcaggaccg gccgcaacgc cgagctgatc
     2101 tcgcgcgact tccgcgtggt gaaggtcgac gtcggcaact tcgaccgcaa cctcgacgtc
     2161 accggcgcct acggcaaccc gaccgcgcac gggatcccgt cggcggtgat cgtctcgccc
     2221 gacaaccgca tcctgttcgc cacccgcgcc ggcgagctcg ccgatgcccg ccgcatgagc
     2281 gaggacggca tccacgactt cttcgccaag gccgccggca ccgtcggcgc cggggcgcat
     2341 tgagcggacc gcgtcagtcc gcgcccgggc gccagcgccg cagcgccagc gcgttgagca
     2401 cgacgctgac gctgctgaac gccatcgccg cgccggccac catcgggctg agcagtccgg
     2461 ccgcggccag gccgatgccg gcgacgttgt agacgaaggc ccagaacagg ttctgccgga
     2521 tcttggcgac gcagcggcgg gagatcgcga gcgcgtcggc caccaggcgc gggtcgttgc
     2581 gcatcagcgt gatgccggcg gcgtgctggg cgacgtcggt gccgccggcc atcgcgatgc
     2641 ccacgtcggc cgcggcgagc gccggggcgt cgttcaggcc gtcgccgacc atcaacacct
     2701 tggctccgcc ggcggccagg gcctccagcg tggcggcttt ctccagcggc gtcaggcccg
     2761 agcgccaggc gtcg
//
LOCUS       DASVLN010000404         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_342385, whole genome shotgun sequence.
ACCESSION   DASVLN010000404 DASVLN010000000
VERSION     DASVLN010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342385"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VF453_09995"
     CDS             <1..659
                     /locus_tag="VF453_09995"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEX7688029.1"
                     /translation="HLGGDLGVSAGRTQSLAPGARARTWALPYAYADFGRLFVREDTF
                     GVRLVPAGWGALELVGRLSTEGEDDAGAGVARRANPRPVGLGTFQETPWGGVFADAFV
                     DTVSGGTRLEVSYAAELGAGAATFYPQAGVSRLDARYARHLYGVDAAEARAAGTRAYA
                     PGTSLAPMAALSGEWALAPHWVLVGHAQREWFGRAISDSPRVGARAQTTAFVALAYRF
                     "
     gene            661..>1623
                     /locus_tag="VF453_10000"
     CDS             661..>1623
                     /locus_tag="VF453_10000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX7688030.1"
                     /translation="MDDAVLWALPVFALLMGAEFAWGVARGRGNYGYADTLASLSQGL
                     LSQLVAACTQAVQVGIYAVLYPHVALAPHAALWTRWEGLVAAVLLYDFCDYWLHRVSH
                     ESAIFWAAHAPHHQSRRFNLSTALRQESAYLLTGWPFFLPLAVVGVPPTLFALAAIVV
                     LFYQFWIHTEHVGSLGALDRWLSTPSNHRVHHATNPRYLDRNYGAILVVWDRLFGSFE
                     PEHADEPCVYGTVVPLAGWDPLRAVAGPYAALFAKARSTPRWRDRLRVLFKSPAWMPP
                     GVAPAPATSDVVDPALPPMRVCVLAGALFLLAVGATAGQLARDDA"
BASE COUNT          169 a          596 c          607 g          251 t
ORIGIN      
        1 atcacctcgg cggcgacctc ggcgtgtcgg ccggccgcac gcagtcgctc gcgcccggcg
       61 ctcgcgcgcg cacgtgggca ctgccctacg cctatgccga cttcgggcgg ctgttcgtgc
      121 gcgaggacac cttcggcgtc cgcctggtgc cggccggctg gggcgcgctc gaactcgtcg
      181 gccgcctcag caccgagggc gaggacgacg ccggcgccgg cgtcgcgcgg cgggcgaacc
      241 cgcggccggt gggtctcggc accttccagg agacgccctg gggcggcgtt ttcgcggacg
      301 cgttcgtcga cacggtctcg ggcggcacgc ggctcgaggt gtcgtatgcc gcggagctgg
      361 gcgccggcgc ggcgacgttc tatccgcagg ccggcgtgtc gcggctggac gcgcgctatg
      421 cccgccacct ctacggcgtc gacgcggccg aggcgcgcgc cgcgggcacg cgcgcgtacg
      481 cgccggggac atcgcttgca ccgatggcgg cgttgtcggg ggaatgggcg ctggcgccgc
      541 actgggtgct ggtcggccat gcgcagcgcg agtggttcgg gcgcgcgatc tccgacagcc
      601 cgcgcgtcgg cgcccgcgcg cagaccacgg ccttcgtcgc gctcgcgtac cggttctgac
      661 atggacgacg ccgttctctg ggccctgccc gtcttcgcgc tgctgatggg cgcggagttc
      721 gcgtggggcg tcgcgcgcgg gcgcggcaac tacggctacg ccgacacgct cgcgagcctc
      781 agccagggcc tgctgagcca gctggtcgcg gcgtgcacgc aggcggtgca ggtcgggata
      841 tacgccgtgc tgtatccgca cgtggcgctc gcgccgcacg cagcgctgtg gacgcgctgg
      901 gagggcctcg tcgcggcggt gctgctgtac gacttctgcg actactggct gcaccgcgtc
      961 tcgcacgaga gcgcgatctt ctgggccgcg catgcgccgc atcaccagag ccggcgcttc
     1021 aacctgtcca ccgcgctgcg ccaggagagc gcctacctgc tcaccggctg gccgttcttc
     1081 ctgccgctgg cggtggtcgg cgtgccgccc acgctgttcg ccctcgcggc gatcgtcgtg
     1141 ctgttctacc agttctggat ccacaccgag cacgtcggct cgctgggcgc gctcgaccgc
     1201 tggctgtcga cgccgtccaa ccatcgcgtg caccacgcga ccaacccgcg ctacctggat
     1261 cgcaactacg gcgcgatcct cgtcgtgtgg gatcgcctgt tcggcagctt cgagccggag
     1321 cacgccgacg agccctgcgt ctacggcacc gtggtgccgc tggccgggtg ggatccgctg
     1381 cgcgcggtcg ccgggcccta cgcggcgctg ttcgccaagg cccggagcac gccgcgctgg
     1441 cgcgaccggc tgcgcgtgct gttcaagtcg ccggcatgga tgccgcccgg ggtcgctcct
     1501 gcgcctgcaa cgagcgacgt cgtggacccc gcgctgccgc cgatgcgcgt gtgcgtgctg
     1561 gcgggggcgc tgttcctgct cgcggtcggt gcgacggcgg gacagctcgc gcgcgacgac
     1621 gcg
//
LOCUS       DASVLN010000405         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_235067, whole genome shotgun sequence.
ACCESSION   DASVLN010000405 DASVLN010000000
VERSION     DASVLN010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235067"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /locus_tag="VF453_10005"
     CDS             <1..1596
                     /locus_tag="VF453_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEX7688031.1"
                     /translation="SRLRALMRALLSLCLASALVSVLAGCASLAEREKTPGFALPPAA
                     DGELARLVPPDLAPGAGSGFRPLAFSSYAMDARLALIRHAQRSLDIQYYLLADDATGR
                     AFLRAVRDAAQRGVRVRILVDELYTASSDRLLQGIAAYPNVQVRLFNPFPAGRALTVT
                     RWGLSLLDLARLNHRMHNKLFIADGIFAIAGGRNIADEYFFRSMRGNFIDLDMLVAGD
                     AVAELAAGFDRYWNSRRVYTLDALEPGAPRADRRRADFEALTAAVADLFPPMTDGKRD
                     FLDFGPLSADMARPPLRLLRGRVQVVTDDPEKVSGQSESGMDPATVTSHVVEAIHDAH
                     DDVLMVSPYVVPGRAALARMLDVRKRQVAVTIVTNSMAANDEPFVSAAYERYRHRLLE
                     MGVQLYEVSPRILRSDKDFDDSFGGMLGDSTGRLHAKVVVIDHERVAVGSMNLDFRSA
                     RENTELGLIVDSPELAGDVTELVDELRSVGTYRMRLGSHDTVQWVDTEPDGEHVFNLE
                     PEVGLFTRLEVILFSPFIDEDLL"
     gene            complement(1621..2082)
                     /locus_tag="VF453_10010"
     CDS             complement(1621..2082)
                     /locus_tag="VF453_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7688032.1"
                     /translation="MKLDRIDIAILECLQRDARMSLQELSAQVGLTASPCWTRIKRME
                     ESGVIDGYSVRVNAEKIGLPDTVIVHVTLDSHSEDALFEFGRALEAIPEVLEAFLVSG
                     DYDYYVRIAVRDTRDYERLLREKLYKIPGIRHSKSSFVLRQLKASAVPLRR"
     gene            2272..>2794
                     /locus_tag="VF453_10015"
     CDS             2272..>2794
                     /locus_tag="VF453_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX7688033.1"
                     /translation="MRAAGPRNGARVVVETLLALGVDTVFGYPGGAVLPLYDALHGEP
                     RLRHVLVRHEQAAVHAAQGYARSTGRPGVVLVTSGPGMSNTTTGLLDALCDSIPVVCL
                     SGQVATAAIGTDAFQECDALGISRPVTKWNAQVRRADALEPMLRKAFAVACGGRPGPV
                     LVDLPKDVQQQPVD"
BASE COUNT          371 a         1025 c          966 g          432 t
ORIGIN      
        1 tcccgcctgc gtgcgctgat gcgcgccctg ctgtcgctgt gcctcgcctc cgcgctcgtg
       61 tcggtgctgg cgggctgcgc gtcgcttgcc gagcgcgaga agacgccggg attcgccttg
      121 ccgccggcgg ccgacggcga actcgcgcgc ctcgtgccgc ccgacctggc gcccggcgcg
      181 ggctcgggct tccggccgct cgccttcagc tcgtacgcga tggacgcgcg cctcgcgctg
      241 atccgccatg cgcagcgcag cctggacatc cagtactacc tgctggccga cgacgcgacc
      301 ggccgcgcgt tcctgcgcgc ggtgcgcgac gccgcgcagc gcggggtgcg cgtgcgcatc
      361 ctcgtcgacg agctctacac cgcctccagc gaccgcctgc tccagggcat cgccgcctat
      421 ccgaacgtgc aggtgcggct gttcaatccg ttccccgcgg ggcgcgcgct caccgtcacg
      481 cgctgggggc tgtcgctgct cgacctcgcg cggctgaacc accgcatgca caacaagctg
      541 ttcatcgccg acggcatctt cgcgatcgcg ggcggccgca acatcgccga cgagtacttc
      601 ttccgcagca tgcgcggcaa cttcatcgac ctcgacatgc tcgtggccgg cgacgcggtg
      661 gccgagctcg ccgccggctt cgaccgctac tggaacagcc gccgcgtcta cacgctggac
      721 gccctcgagc ccggcgcccc gcgggccgac aggcggcgcg cggacttcga ggcgctcacc
      781 gcggccgtcg ccgacctgtt cccgccgatg accgacggca agcgcgactt cctcgacttc
      841 ggccccctca gcgcggacat ggcgcgcccg ccgctgcgcc tgctgcgcgg ccgcgtgcag
      901 gtcgtgacgg acgatcccga gaaggtcagc ggccagagcg agtccggcat ggatccggcg
      961 acggtgacct cgcacgtcgt cgaggccatc cacgacgcgc acgacgacgt gctgatggtc
     1021 tcgccctacg tcgtgccggg gcgggcggcg ctcgcgcgca tgctggacgt gcgcaagcgc
     1081 caggtcgcgg tgaccatcgt caccaactcg atggcggcca acgacgagcc cttcgtcagc
     1141 gccgcctacg agcgctaccg ccaccgcctg ctggagatgg gcgtgcagct ctacgaggtc
     1201 agcccgcgca tcctgcgcag cgacaaggac ttcgacgact ccttcggcgg aatgctgggc
     1261 gactccaccg gccgcctgca cgccaaggtc gtcgtgatcg accacgagcg cgtcgccgtc
     1321 ggctcgatga acctcgactt ccgctccgcg cgcgagaaca cggagctcgg cctgatcgtc
     1381 gactcgcccg agctcgcggg cgacgtcacc gagctcgtcg acgaactgcg ctccgtcggc
     1441 acctaccgca tgcgcctggg ctcccacgac accgtgcagt gggtcgacac cgagcccgac
     1501 ggcgagcatg tcttcaacct cgagcccgag gtcggcctct tcacgcggct cgaggtgatc
     1561 ctgttctcgc cgttcatcga cgaggacctg ctgtgagcgc ggacgcccgc gccgcggcgc
     1621 tcaccgcctg agcggcaccg cgctcgcctt cagctgccgc agcacgaagc tcgacttgct
     1681 gtggcggatg ccggggatct tgtacagctt ctcgcgcagc aggcgctcgt agtcgcgggt
     1741 gtcgcgcacg gcgatgcgga cgtagtagtc gtagtcgccg gagaccagga aggcctccag
     1801 cacctcgggg atggcctcga gcgcgcgccc gaactcgaac agggcgtcct cggaatggct
     1861 gtccagcgtg acgtgcacga tgacggtgtc gggcaggccg atcttctcgg cgttcacgcg
     1921 caccgagtag ccgtcgatga cgcccgattc ctccatgcgc ttgatgcgcg tccagcaggg
     1981 tgaggcggtc aggccgacct gggcgctcag ctcctgcagg ctcatgcgcg cgtcgcgttg
     2041 caggcattcc aggatcgcga tgtcgatgcg gtctagcttc atgccgggat tctgcacgat
     2101 ggcggaagga ttttccgttc tgtcacgtca atgcggaaga tttgaaagcc tttttcgcgc
     2161 catcgtccgc aaactgcggg catgaccgcc accgtcctgt cgcccgttcc gccgccgtcg
     2221 ccgggggccg gccttcgttg cgtcgttccg tccgcgggat cggacccgcc cgtgcgcgcg
     2281 gcgggacccc gcaacggcgc gcgcgtcgtc gtggagacgc tgctcgcgct gggcgtcgat
     2341 acggtgttcg ggtatccggg cggcgccgtg ctgccgctgt acgacgcgct gcacggcgag
     2401 ccgaggctgc gccacgtgct cgtgcggcac gagcaggccg cggtgcatgc ggcccagggg
     2461 tatgcgcgca gcaccggccg gccgggcgtg gtgctggtga cctcgggccc cggcatgagc
     2521 aacacgacga ccggcctgct ggacgcgctg tgcgactcga tcccggtcgt ctgcctgagc
     2581 ggccaggtgg cgaccgcggc catcggcacc gacgcgttcc aggagtgcga cgcgctgggc
     2641 atctcgcgcc ccgtgaccaa gtggaacgcc caggtgcgcc gggcggacgc gctcgagccc
     2701 atgctgcgca aggccttcgc cgtggcctgc ggcgggcggc cggggccggt gctggtcgac
     2761 ctgccgaagg acgtgcagca gcagccggtc gacg
//
LOCUS       DASVLN010000406         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_290207, whole genome shotgun sequence.
ACCESSION   DASVLN010000406 DASVLN010000000
VERSION     DASVLN010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290207"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..863)
                     /gene="asd"
                     /locus_tag="VF453_10020"
     CDS             complement(15..863)
                     /gene="asd"
                     /locus_tag="VF453_10020"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655818.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEX7688034.1"
                     /translation="MSDRLAVLPQYLLPKRLLTSLAGRVAGARAGGVTTALIRRFIAR
                     YGVDMAEALESDPAAYATFNEFFTRALKAGARPLARADLVCPVDGAISQFGRIDEHRI
                     VQAKGHAFTTQALVGGDAALAEPFVHGSFANLYLSPKDYHRIHMPCDGRLRRMVYVPG
                     ALFSVNPTTARGVPGLFARNERVVCLFDGDRGPWVLVLVGATIVGSMAVAWHGVVNPP
                     RPGVLREWRYDERDVVLRQGDEMGRFLLGSTVVMLFPKPGLVFNPAWAPERAVRLGEA
                     MADYPA"
     gene            complement(1152..2267)
                     /gene="ald"
                     /locus_tag="VF453_10025"
     CDS             complement(1152..2267)
                     /gene="ald"
                     /locus_tag="VF453_10025"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX7688035.1"
                     /translation="MKIGVPKEIKNHEYRVGLTPASVREFVAHGHAVLVQAGAGAAIG
                     LDDAQYAAAGATIAPDAAAVFAQADMIVKVKEPQAQECAMLRPGQLLYTYLHLAPDPE
                     QTAALVKSGAVCVAYETITGPNGGLPLLAPMSEVAGRMAIQAGAAHLEKSKGGMGVLL
                     GGVPGVAPADVTILGAGVVGTHALQVAVGVGARVTVLDKNVDRLRQLDLVFGNRITTL
                     YSSAQSVEESVLRADLVVGGVLIPGAAAPKLVTRDMISRMKKGAVVVDVAIDQGGCFE
                     TSHATTHAEPTYVVDGVVHYCVANMPGAVARTSTFALNNATIAHGLALADHGWRAAMQ
                     ANAHLKAGLNVAEGRVTFEAVARDLGYDYTPADALLR"
     gene            2397..3344
                     /locus_tag="VF453_10030"
     CDS             2397..3344
                     /locus_tag="VF453_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HEX7688036.1"
                     /translation="MNLLDPAALECLAALAEAGSFERAAQLLSITQSAVSQRLRALET
                     QVGQLLVVRSRPLRMTEAGKVLLRYARQLQALRADAARELGASQSREERLPIAVNADS
                     LATWVLGALDALVHAGQREGYGLELVVDDQDFTHDRLREGEVLGCVSTVSMALRGCSC
                     QALGRMRYIAVASPAFVAQAMPEGLNEANFAKLPFIVFNRKDDMQAQWVARAMGVREP
                     RLKERFVPSSEAGARAAVMGWGVAVIPELLARPHLDAGALVAIRADVAIDVALYWHQW
                     KLGGADAAADAGRPPARPGLMEQIGQALIAGARASLGAV"
     gene            3369..4295
                     /locus_tag="VF453_10035"
     CDS             3369..4295
                     /locus_tag="VF453_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7688037.1"
                     /translation="MLILETPRLRLRWFTAADADFIRRLLNDPGWIANIGQRNVRTHR
                     QAQAWIASRLTAVYGRLGFGFWAVERKEDGALAGMCGLIKRDSLPVVDVGYALMPAFR
                     GQGYAREAAAACLRYAREVLGLAEVWAIASPGNEASAAVLRGIGLSDAGVERLPGESR
                     DSRVFKAPPADLGDDRAQLDGLTRRFLAAFTNRGGAIPTLAALPHLFLADATIRSADA
                     QGTLTSTGVHGFIAPRAELLLRGRLEDFEEHETGGRTEIAGALAQRRLRYAKRGTLDG
                     APFEGAGTKMLQFARTALGWKIAALQWTDDPA"
     gene            4324..>4918
                     /gene="pth"
                     /locus_tag="VF453_10040"
     CDS             4324..>4918
                     /gene="pth"
                     /locus_tag="VF453_10040"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX7688038.1"
                     /translation="MIKLIVGLGNPGPEYEDTRHNAGFWWVEQAVRQLGGHLAHDPKY
                     HGFVARVNRPAGPVWLQQPLTYMNLSGKAVAPLARFFKILPDEILVVHDELDILPGQM
                     KLKKGGGAGGHNGIKDIQAQLGTPDFWRLRLGIGHPGHKGEVAAYVLRKPPASERLLI
                     DGNVDESLKCLDLLLAGEMDKAMMKIHAKPQRPKPPRP"
BASE COUNT          659 a         1798 c         1806 g          655 t
ORIGIN      
        1 gcccgacgcg cgcgtcaggc cggatagtcg gccatcgcct cgcccaggcg cacggcgcgc
       61 tccggcgccc aggccggatt gaagacgagg cccggcttcg ggaacagcat cacgaccgtc
      121 gagccgagca ggaagcggcc catctcgtcg ccctggcgca ggacgacgtc gcgctcgtcg
      181 tagcgccact cgcgcaggac gccgggccgc ggcggattga ccacgccgtg ccacgccacc
      241 gccatgctgc cgacgatggt cgcgcccacc agcacgagca cccaggggcc gcggtcgccg
      301 tcgaacaggc acacgacgcg ctcgttgcgg gcgaacaggc ccggcacgcc gcgcgcggtg
      361 gtcgggttca ccgagaacag cgcgccaggc acgtacacca tgcggcgcag gcggccgtcg
      421 cagggcatgt ggatgcggtg gtagtccttg ggactcaggt acaggttggc gaagctgccg
      481 tgcacgaagg gctcggcgag cgccgcgtcg ccgccgacca gggcctgggt cgtgaaggcg
      541 tggcccttcg cctggacgat ccggtgctcg tcgatgcggc cgaactggct gatcgcgccg
      601 tcgaccgggc agacgaggtc ggcccgcgcc agcggccgtg cgccggcctt gagcgcgcgc
      661 gtgaagaact cgttgaaggt cgcgtaggcg gccgggtcgg actcgagcgc ctcggccatg
      721 tccacgccgt agcgggcgat gaagcgccgg atcagcgccg tcgtcacccc gcccgcgcgc
      781 gcgccggcga cacggccggc cagcgaagtg agcagccgct tgggcagcag gtattggggg
      841 aggacggcga ggcggtcgga catggcttgt tcttcttcgc aggcggccgg caagcggcgg
      901 gccgcggggc ccggcatccg cggcgccccc cggaggcacg cggcgcggcc gcgtcgtccc
      961 gacgggctgc tcgcgaggcc ttcagtcgag cggtgattgt accggggccg ccggcgccgc
     1021 cccccggctc tcgcgaccgg gaagatcggt gccgtcgatg cgaacgcccc catcgcgagc
     1081 gagcacgccg gggagccggt cgcccctgcg cgatcgagcg cgtcgggcgg ctcgaccggc
     1141 gccggggccg ctcagcgcag cagtgcgtcg gccggggtgt agtcgtagcc caggtcgcgc
     1201 gcgacggcct cgaaggtcac gcggccctcg gcgacgttca ggcccgcctt caggtgcgcg
     1261 ttggcctgca tcgccgcgcg ccagccgtgg tcggcgagcg cgagcccgtg ggcgatcgtc
     1321 gcgttgttca gcgcgaaggt ggaggtgcgc gccaccgcgc cgggcatgtt ggcgacgcag
     1381 tagtgcacga cgccgtcgac gacgtaggtc ggctcggcgt gcgtggtcgc gtgcgaggtc
     1441 tcgaagcagc cgccctggtc gatcgccacg tcgaccacca ccgcgccctt cttcatgcgc
     1501 gagatcatgt cgcgcgtcac cagcttgggc gcggccgcgc ccgggatcag cacgccgccg
     1561 acgaccaggt cggcgcggag caccgattcc tcgacggact gcgcgctcga gtacagcgtc
     1621 gtgatgcggt tgccgaacac gaggtccagc tggcgcaggc ggtcgacgtt cttgtccagc
     1681 acggtcacgc gcgcgcccac gccgaccgcg acctgcagcg cgtgcgtgcc gaccacgccc
     1741 gcgccgagga tggtcacgtc ggccggcgcc acgccgggca cgccgcccag cagcacgccc
     1801 atgccgccct tgctcttctc gaggtgcgcc gcgccggcct ggatggccat gcgccccgcg
     1861 acctcgctca tcggcgcgag cagcggcagg ccgccgttcg gcccggtgat cgtctcgtag
     1921 gccacgcaca ccgcacccga cttcacgagc gccgccgtct gctcgggatc cggggcgagg
     1981 tggaggtagg tgtagagcag ctggccgggg cgcagcatcg cgcactcctg cgcctgcggc
     2041 tccttgacct tgacgatcat gtccgcctgc gcgaacaccg ccgcggcatc gggggcgatc
     2101 gtcgcgccgg cggccgcgta ctgcgcgtcg tccagcccga tcgccgcgcc ggcgccggcc
     2161 tgcaccagca ccgcgtggcc gtgggcgacg aattcccgca cgctcgccgg cgtcaggccg
     2221 acgcggtact cgtggttctt gatctccttc ggaacgccga tcttcatggg atgtctccgt
     2281 gatgtgggtc gtcgccgcgg ccctcattgc ggcccgcgtg cgtggttcca tcgtaggcag
     2341 cggccgtgcg gcggtgaagg tgcatcgcaa ttactgcgat tacagtagcg gcacttatga
     2401 acctgctcga tccggccgcc ctcgaatgcc tggccgcgct cgccgaggcg ggcagcttcg
     2461 agcgcgcggc gcagctgctg tcgatcacgc agtcggcggt ctcgcagcgg ctgcgggcac
     2521 tggagacgca ggtggggcag ctgctggtcg tgcgatcgcg gccgctgcgc atgaccgagg
     2581 ccggcaaggt gctgctgcgc tacgcccgcc agctgcaggc gctgcgcgcg gacgcggcgc
     2641 gcgagctcgg cgccagccag tcccgcgagg agcgcctgcc catcgccgtc aacgccgaca
     2701 gcctcgcgac ctgggtgctg ggcgcgctcg acgcgctcgt gcatgccggc cagcgcgagg
     2761 gctacggcct cgagctcgtc gtcgacgacc aggacttcac gcacgaccgc ctgcgcgagg
     2821 gcgaggtgct cggctgcgtg agcaccgtct cgatggcgct gcggggctgc agctgccagg
     2881 cgctcgggcg catgcgctac atcgccgtcg cgagcccggc gttcgtcgcg caggcgatgc
     2941 ccgagggcct gaacgaggcg aacttcgcga agctgccctt catcgtcttc aaccgcaagg
     3001 acgacatgca ggcccagtgg gtcgcgcgcg cgatgggcgt gcgcgagccg cggctcaagg
     3061 agcgcttcgt cccctccagc gaggccggcg cgcgcgccgc ggtgatgggc tggggcgtcg
     3121 cggtgatccc ggagctgctc gcccgcccgc acctcgacgc gggcgcgctg gtcgcgatcc
     3181 gtgcggacgt ggcgatcgac gtcgcgctgt actggcacca gtggaagctg ggcggcgccg
     3241 atgccgccgc ggacgccggc cgcccgcccg cgcggcccgg gctgatggag cagatcgggc
     3301 aggcgctcat cgccggcgcg cgcgcttcgc tcggcgccgt gtgagcgccg gcccgatact
     3361 ctcgccccat gctgatcctc gagactcccc gcctgcgcct gcgctggttc accgccgccg
     3421 acgccgactt catccgccgg ctcctcaacg atccgggctg gatcgccaac atcggccagc
     3481 gcaacgtgcg cacgcaccgg caggcgcagg cctggatcgc gtcgcgcctg accgcggtct
     3541 acgggcggct cggcttcggc ttctgggccg tcgagcgcaa ggaggacggc gcgctcgccg
     3601 gcatgtgcgg gctgatcaag cgcgactcgc tgcccgtggt cgacgtcggc tatgcgctga
     3661 tgccggcgtt ccgcggccag ggctacgcgc gcgaggcggc cgccgcgtgc ctgcgctacg
     3721 cgcgcgaggt gctggggctg gccgaggtgt gggccatcgc gtcgccgggc aatgaggcct
     3781 cggccgcggt gctgcgcggc atcggcctga gcgacgccgg cgtcgagcgc ctgccgggcg
     3841 agagccgcga ctcccgcgtc ttcaaggcgc cgcctgccga cctcggcgac gaccgcgcgc
     3901 agctcgacgg cctcacccgc cgcttcctcg cggcgttcac gaaccgcggc ggcgcgatcc
     3961 cgacgctggc ggcgctgccg cacctgttcc tcgccgacgc cacgatccgc agcgcggacg
     4021 cccagggcac gctcacgtcc acgggcgtgc acggcttcat cgccccgcgc gccgagctgc
     4081 tgctgcgcgg acggctggag gatttcgagg agcacgagac cggcggccgc accgagatcg
     4141 ccggcgcgct cgcccaacgc cgcctgcgct acgccaagcg cggcacgctg gacggcgcgc
     4201 cgttcgaggg cgcgggcacg aagatgctgc agttcgcgcg caccgcgctg ggctggaaga
     4261 tcgcggcgct gcagtggacg gacgacccgg cctgagccgc ccgtcgcccg ggataatcgc
     4321 gggatgatca agctcatcgt cggcctgggc aacccgggcc cggaatacga ggacacgcgc
     4381 cacaacgccg gcttctggtg ggtcgagcag gccgtccggc aactgggcgg gcatctcgcc
     4441 cacgatccga agtaccacgg cttcgtcgcg cgcgtgaacc ggcccgcggg accggtctgg
     4501 ctgcaacagc cgctgaccta catgaacctc tcgggcaagg cggtggcgcc gctcgcgcgc
     4561 ttcttcaaga tcctgcccga cgagatcctc gtcgtgcacg acgagctcga catcctgccc
     4621 gggcagatga agctcaagaa gggcggcggc gccggcggcc acaacggcat caaggacatc
     4681 caggcccagc tcggcacgcc ggatttctgg cgcctgcgcc tgggcatcgg ccatccgggc
     4741 cacaagggcg aggtcgccgc gtacgtgctg cgcaagccgc ccgcgtcgga acggctgtta
     4801 atcgacggaa atgtcgacga gagcctgaaa tgcctcgatc tgctcctggc gggcgagatg
     4861 gacaaggcga tgatgaagat ccacgccaag ccgcagcgcc cgaagccgcc gcgccccg
//
LOCUS       DASVLN010000407         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_128760, whole genome shotgun sequence.
ACCESSION   DASVLN010000407 DASVLN010000000
VERSION     DASVLN010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128760"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..>1943
                     /locus_tag="VF453_10045"
     CDS             120..>1943
                     /locus_tag="VF453_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7688039.1"
                     /translation="MNWFNRLKLAHKLLITFLACAALTAAVGVLGFLRASDLGRTLEG
                     TYENGVVPLQLVGEAGANLQAHARNYVRLPGLKDAKDVQDDVDRAKGRLDKFRKAVDA
                     YRKTDLAPREQELVKQLDDLMPRYLATCDRIAESVSAGRTADAVALGNGELRKETDDI
                     EALLDGLADELRTQAKAADDEAARAVASTRDMLMGGVAVAVVFAIAICLVVTRAVMRQ
                     LGGDPAYAAGLLRRVADGDLSAQIELRAGDDSSLLFALRQTVARLAQVIEGQNRLVAA
                     ANRGQFDARVELASLQGFQKEMGEGLNTLVETTGGSIRDVVDVMLALSEGDLTRTIDR
                     DYQGAFGEMKQYVNQTVAKLSQVVAEVNGSAEALAGASEEVSATAQSLSQAASEQAAG
                     VEETSASIEQMTASISQNTENAKVTDGMAAKASAEASEGGEAVKATVVAMKQIAQKIG
                     IIDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRKLAERSQVAAEEIANVAGSS
                     VELAEKAGRLLETIVPNIRKTSDLVQEIAAASEEQTSGVSQINVAMTQLSQTTQQNAS
                     SSEELAATAEEMSSQAEQLQQAMAFFRVASADAPAPRAVARS"
BASE COUNT          327 a          696 c          695 g          225 t
ORIGIN      
        1 gcgcggacga ggcgatcgac gagacgcagt tcagccgctt ctgagccccc ggcgcggcgc
       61 ccgaccgggc gcgcgccgcg atccatccaa cgacgacatc ccgaccggag agcatgcccg
      121 tgaactggtt caaccgcctc aagctcgccc acaagctgct gatcaccttc ctcgcctgcg
      181 ccgcgctcac cgcggcggtg ggcgtgctcg gcttcctgcg ggcgtccgac ctgggccgca
      241 ccctggaagg cacctacgag aacggcgtcg tgccgctgca gctggtcggc gaggcgggtg
      301 ccaacctgca ggcgcacgcg cgcaactacg tgcgcctgcc cgggctgaag gacgccaagg
      361 acgtgcagga cgacgtcgac cgcgccaagg gccgcctcga caagttccgc aaggccgtcg
      421 atgcctaccg aaagaccgac ctcgccccgc gcgagcagga gctggtcaag cagctcgacg
      481 acctgatgcc gcgctacctc gccacctgcg accgcatcgc cgagagcgtc tccgccggcc
      541 gcacggccga cgcggtcgcg ctgggcaacg gggagctgcg caaggagacg gacgacatcg
      601 aggcgctgct ggacggcctg gccgacgagc tgcgcacgca ggccaaggcg gccgacgacg
      661 aggccgcccg cgcggtcgcc agcacgcgcg acatgctgat gggcggcgtc gcggtggcag
      721 tcgtgttcgc gatcgcgatc tgcctcgtcg tcacgcgcgc cgtgatgcgc cagctgggcg
      781 gcgacccggc ctacgccgcg ggcctgctgc ggcgcgtggc cgacggcgac ctgtccgcgc
      841 agatcgagct gcgcgcgggc gacgactcga gcctgctgtt cgcgctgcgc cagacggtcg
      901 cccgcctggc gcaggtcatc gagggccaga accgcctcgt ggcggcggcc aaccgcggcc
      961 agttcgacgc gcgcgtcgag ctcgccagcc tgcagggctt ccagaaggag atgggcgagg
     1021 gcctgaacac gctggtcgag accaccggcg gcagcatccg cgacgtcgtg gacgtgatgc
     1081 tcgcgctgtc ggagggcgac ctcacccgaa ccatcgaccg cgactaccag ggcgcgttcg
     1141 gcgagatgaa gcagtacgtc aaccagacgg tggccaagct ctcgcaggtg gtcgccgagg
     1201 tcaacggcag cgccgaggcg ctggcgggtg cgtccgagga ggtcagcgcg acggcgcagt
     1261 cgctgtcgca ggccgccagc gagcaggccg ccggcgtcga ggagaccagc gcctcgatcg
     1321 agcagatgac ggcgtcgatc tcgcagaaca cggagaacgc caaggtcacc gacggcatgg
     1381 ctgccaaggc ctcggccgag gcgagcgaag gcggcgaggc cgtcaaggcg accgtcgtcg
     1441 ccatgaagca gatcgcgcag aagatcggca tcatcgacga catcgcctac cagaccaacc
     1501 tgctcgcgct caacgccgcc atcgaggctg cgcgcgcggg cgagcatggc aagggcttcg
     1561 ccgtcgtcgc cgccgaggtg cgcaagctcg ccgagcgcag ccaggtcgcc gccgaggaga
     1621 tcgccaacgt cgcgggctcg agcgtcgagc tcgccgagaa ggccggccgc ctgctggaga
     1681 ccatcgtccc caacatccgc aagacctccg acctcgtgca ggagatcgcc gcggcgtccg
     1741 aggagcagac ctcgggcgtt agccagatca acgtcgcgat gacccagttg agccagacga
     1801 cgcagcagaa cgcgtccagc tcggaggagc tcgccgcgac cgccgaggag atgagctcgc
     1861 aggcggagca gttgcaacag gcgatggcgt tcttccgcgt cgcctccgcc gacgcgccgg
     1921 cgccgcgggc cgtggcgcgc tcg
//
LOCUS       DASVLN010000408         5626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_505669, whole genome shotgun sequence.
ACCESSION   DASVLN010000408 DASVLN010000000
VERSION     DASVLN010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505669"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1772
                     /gene="edd"
                     /locus_tag="VF453_10050"
     CDS             <1..1772
                     /gene="edd"
                     /locus_tag="VF453_10050"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEX7688040.1"
                     /translation="SAATRADYLGHVDALRHRARGPERLGCANVAHAFAALPANDKLR
                     VVAERAPHIGVVTAYNDMLSAHQPYEHYPDLIRATARALGATAQVAGGVPAMCDGVTQ
                     GAPGMELSLFSRDTIAMGTAIALSHDVFDAVLMLGICDKIVPGLLIGALQFGHLPCVF
                     VPAGPMSSGLSNNEKAKVREKAAQGLVGRPELLEAESQAYHGPGTCTFYGTANSNQML
                     LEAMGLHVPGAAFVHPHDDLRTALTKEAVATVLSMVRGKRDLPIAEIVDERCIVNAMI
                     ALLATGGSTNHLIHWVAVARAAGILIDWSDFAELSTVIPLLARVYPNGSADVNQFQAA
                     GGPGFVLRELRDAGLLHPDVNTVSEGGGAAYADVPEMVDGRLGWKVLPRAPIDAGVVR
                     TAAEPFSATGGLKLLEGNLGRSVIKVSAVPEDRHVVTAPARVFDSQEDLLHAFQRGEL
                     EQDFVAVVRWQGPRANGMPELHKLTPPLAVLQGRGFKVALVTDGRMSGASGKVPAAIH
                     VSPEALGGGPLARVRDGDPIRLDAIAGTLQVLVPAADWDARVPAERPQALADSHGAGF
                     GRTLFAGMRRNVTTAEEGACTWL"
     gene            1816..2439
                     /gene="eda"
                     /locus_tag="VF453_10055"
     CDS             1816..2439
                     /gene="eda"
                     /locus_tag="VF453_10055"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX7688041.1"
                     /translation="MNPLDLASHGPVIPVIVVDRLADAVPMARALVAGGVRVLEVTLR
                     TEAGLPAIQAIAREVPEAIVGAGTVRNAADARAARDAGARFIVSPGYTPAVGAACREL
                     GVALLPGVSTGSEIMMAADDGYTFLKLFPATAVGGIPLLKAFAGPFGDIAFCPTGGIT
                     VETAPQFLALPNVKVVGGSWLTPAEALKAGDWDRVTALAKAASALRG"
     gene            2520..2939
                     /locus_tag="VF453_10060"
     CDS             2520..2939
                     /locus_tag="VF453_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7688042.1"
                     /translation="MASIRKEFEIDVPVEVAWAAFRDVGAVHERLARGFVVDCRLDGP
                     DRVVTFANGFVAREMIVDLDDRRRRLAYSARSERLAHHHASFELADAGSGRTRVRWIA
                     DLLPDAAAAVVGAMMEDGSAAIRRTLAAVGCRLSAVG"
     gene            2957..3796
                     /locus_tag="VF453_10065"
     CDS             2957..3796
                     /locus_tag="VF453_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7688043.1"
                     /translation="MRSRTLFRGRGIAVSEFVCDAGPQDRPFTEMHAAYSVAFVRAGS
                     FGCRTDAAYRELVPGAALAGRPGAEYRCTHEHHARGDECLCVELSPELVDAVCGLDRW
                     RAGALPPLPELVVRGGLLQAAADGRSDIAIEEAALLFAQAYARAGEAQARPACVDRHA
                     RRRAVEVAHWIDDHAGEAVSLEDAAGRAGLSAFHFLRVFSATLGVTPHQHLLRARLRR
                     AARLLAEEDRPITDVALECGFADLSNFVRTFRRAAGLPPAAFRRASRGERRIVQDALG
                     RAA"
     gene            3885..4247
                     /locus_tag="VF453_10070"
     CDS             3885..4247
                     /locus_tag="VF453_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7688044.1"
                     /translation="MMFDHVGLRVTDLEASARLYAACLAPLGFVPGSAGEDYAGFGPP
                     QASALWLHRDASARKGAHVAFAAPDRAAVDRFHAEGLKAGARDNGAPGLRPDYSPTYY
                     AAFLLDLDGNNIEAVCTR"
     gene            complement(4248..4898)
                     /locus_tag="VF453_10075"
     CDS             complement(4248..4898)
                     /locus_tag="VF453_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX7688045.1"
                     /translation="MYQPASFVEQDPEQLAALMHAAPLATLVRGGPELAADVIPLMAE
                     RTDTGWRLAGHVARANPLWREADGQAVLAVFHGAQAYVSPNWYPSKFQHGKMVPTWNY
                     SLVQAHGRLRAIDDPAWLRAFLERLTDRHEGGRPVPWHVSDAPEDYLAATLRAIVGIE
                     IEVTRLEGKRKLSQNRSADDQAGVVRGLEADAASGIQPQAARVAADMREAQRRAGG"
     gene            complement(4926..>5626)
                     /locus_tag="VF453_10080"
     CDS             complement(4926..>5626)
                     /locus_tag="VF453_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7688046.1"
                     /translation="LARDGARVALWDVADAAGQALADELRAAGHGAIYAHCNVASKAE
                     VDAALAAAVREFGAVDGLVNNAGIFRAADFLDITEADWDAVIDVNLKGSFLVGQAVAR
                     QMAAQRRGAIVNMSSVNGLMAIPTIASYNASKGAINQLTRAMALGLIDHGVRVNAVAP
                     GTISTELARNSVLTSDAARQRILGRTPMKRLGEPEEVADVVCWLLSDASSYVTGEVVY
                     VDGGRLALNYTVPV"
BASE COUNT          706 a         2091 c         2051 g          778 t
ORIGIN      
        1 gcagcgccgc gacgcgggcc gactacctcg gccacgtgga cgcgctgcgc caccgcgcgc
       61 gcggcccgga gcgcctgggc tgcgccaacg tcgcccacgc gttcgcggcg ctgccggcca
      121 acgacaagct gcgcgtggtc gcggagcgcg caccccacat cggcgttgtc acggcctaca
      181 acgacatgct gtcggcccac cagccctacg agcactaccc cgacctcatc cgcgccaccg
      241 cccgcgcgct cggcgcgacg gcgcaggtcg ccggcggcgt gccggccatg tgcgacggcg
      301 tgacgcaggg cgcgcccggc atggagctgt cgctgttctc gcgcgacacc atcgcgatgg
      361 gcacggcgat cgcgctgtcg cacgacgtgt tcgacgccgt gctgatgctg ggcatctgcg
      421 acaagatcgt gcccggcctg ctgatcggcg cgctgcagtt cggccacctg ccgtgcgtgt
      481 tcgtgcccgc ggggccgatg tccagcggcc tgtcgaacaa cgagaaggcc aaggtgcgcg
      541 agaaggccgc gcagggcctg gtcgggcggc cggagctgct cgaggccgag agccaggcct
      601 accacggccc cggcacctgc accttctacg gcacggccaa ctccaaccag atgctgctcg
      661 aggcgatggg gctgcacgtg ccgggcgccg cgttcgtcca tccgcacgac gacctgcgca
      721 ccgcgctcac gaaggaagcc gtcgcgacgg tgctgtcgat ggtgcgcggc aagcgcgacc
      781 tgccgatcgc cgagatcgtc gacgagcgct gcatcgtcaa cgcgatgatc gcgctgctgg
      841 cgaccggcgg ctcgaccaac cacctgatcc actgggtcgc cgtcgcgcgc gcggcgggca
      901 tcctgatcga ctggtcggac ttcgcggagc tgtccaccgt gatcccgctg ctcgcccgcg
      961 tctacccgaa cggcagcgcc gacgtgaacc agttccaggc cgcgggcggc ccgggcttcg
     1021 tgcttcgcga gctgcgcgac gccgggctgc tgcatccgga cgtgaacacc gtcagcgagg
     1081 gcggcggcgc ggcctacgcg gacgtgccgg agatggtcga cggccgtctc ggctggaagg
     1141 tgctgccgcg cgcgcccatc gacgcgggcg tcgtgcgcac cgcggccgag ccgttcagcg
     1201 ccaccggcgg cctgaagctg ctcgagggca acctcggccg ctcggtcatc aaggtctcgg
     1261 cggtgccgga ggatcgccac gtcgtcaccg cgccggccag ggtcttcgat tcgcaggagg
     1321 acctgctgca cgccttccag cgcggcgagc tcgagcagga cttcgtcgcg gtcgtgcgct
     1381 ggcaggggcc gcgcgccaac ggcatgcccg agctgcacaa gctcacgccg ccgctggcgg
     1441 tgctgcaggg acggggcttc aaggtcgcgc tcgtcaccga tggccgcatg agcggcgcgt
     1501 cgggcaaggt gccggcggcg atccacgtca gcccggaggc gcttggcggc ggcccgctcg
     1561 cgcgcgtgcg cgacggcgat ccgatccggc tggacgccat cgccggcacc ctgcaggtgc
     1621 tggtgcccgc ggccgactgg gacgcccgcg tgcccgccga acggccgcag gcactcgccg
     1681 attcccacgg cgccggcttc ggccgcacgc ttttcgccgg catgcgccgc aacgtcacca
     1741 ccgccgagga aggcgcctgc acctggctct gaagggccgc tgccgcagcg cccgacccgc
     1801 aacacgaagg acaccatgaa cccgctcgac ctcgccagcc acggccccgt catccccgtc
     1861 atcgtcgtcg accgcctcgc ggacgccgtg cccatggcgc gtgcgctcgt cgccggcggc
     1921 gtgcgcgtgc tggaggtgac gctgcgcacc gaggccggcc tgccggcgat ccaggccatc
     1981 gcgcgcgagg tgccggaggc catcgtcggc gccggcaccg tgcgcaacgc ggccgacgcc
     2041 cgtgccgccc gcgacgccgg cgcgcgcttc atcgtcagcc cgggctacac gccggcggtc
     2101 ggcgcggcct gccgcgagct gggcgtcgcg ctgctgccgg gcgtgtccac cggcagcgag
     2161 atcatgatgg ccgccgacga cggctacacc ttcctcaagc tgttcccggc caccgcggtc
     2221 ggcggcatcc cgctgctgaa ggcgttcgcc gggccgttcg gcgacatcgc gttctgcccg
     2281 accggcggca tcaccgtcga gaccgcgccg cagttcctcg cgctgcccaa cgtgaaggtc
     2341 gtcgggggat cgtggctcac gccggccgag gcgctgaagg cgggtgactg ggatcgcgtc
     2401 acggcgctcg cgaaggcggc gtccgcgctg cgcggctgac cgtcccgccc cggagcgcaa
     2461 ttttctccaa gcggccgcgg cgccgcgcac gctatggtcg cctgcttcga ggagaacgca
     2521 tggcttccat ccgcaaggaa ttcgagatcg acgtgcccgt cgaggtcgcc tgggccgcct
     2581 tccgtgacgt cggtgccgtg cacgagcgcc tcgcgcgcgg cttcgtcgtc gactgccgcc
     2641 tcgacggtcc ggatcgcgtc gtcacgttcg cgaacggctt cgtcgcgcgc gagatgatcg
     2701 tcgacctcga cgaccgccgc cgccggctcg cctactcggc gcgcagcgag cgcctggccc
     2761 accaccacgc cagcttcgag ctggccgacg ccggcagcgg ccgcacgcgc gtgcggtgga
     2821 tcgccgacct gctgcccgac gccgcggcgg ccgtcgtcgg tgcgatgatg gaggacggca
     2881 gcgcggcgat ccgacggacg ctcgcggctg tcggctgtcg gctgtcggct gtcggctgac
     2941 cggcgggagg tgacgcatgc gatcgaggac tctgttccgg ggccgcggca tcgcggtgtc
     3001 cgagttcgtc tgcgatgccg gcccgcagga tcgtccattc accgagatgc acgccgcgta
     3061 cagcgtcgcg ttcgtgcgcg ccggcagctt cggctgccgt accgacgctg cgtaccgcga
     3121 gctggtgccg ggcgcggcgc tcgcgggccg gccgggggcg gagtatcgct gcacgcacga
     3181 gcaccacgcc cgcggcgacg agtgcctgtg cgtcgagctg tcgcccgagc tcgtcgacgc
     3241 ggtctgcggc ctcgaccgct ggcgtgcggg cgcgttgccg ccgctgccgg agctggtcgt
     3301 gcgcggcggg ctgctgcagg ctgccgcgga cggccgcagc gacatcgcga tcgaggaggc
     3361 cgcgctgctg ttcgcgcagg cttacgcgcg cgccggcgag gcgcaggcgc ggccggcctg
     3421 cgtcgaccgc cacgcccgtc gccgcgcggt cgaggtggcg cactggatcg acgaccacgc
     3481 cggcgaggcg gtctcgctcg aggacgccgc cggccgcgcg ggcctgagcg cgttccactt
     3541 cctgcgcgtg ttctcggcca cgctcggcgt cacgccgcac cagcacctgc tgcgtgcccg
     3601 gctgcggcgc gccgcgcgcc tgctggccga ggaggatcgc ccgatcaccg acgtggcgct
     3661 ggagtgcggc ttcgccgacc tgtccaactt cgtgcgcacc ttccgccgcg ccgccggcct
     3721 gccgcccgcg gcgttccggc gcgcgagccg cggcgagcgc aggattgtcc aagacgcgct
     3781 cggtcgcgct gcctagagtc ctgcaccagg acgacgcccg acccgcgggc cgtcgacgag
     3841 ccaccgcccg cgcccaccgc gggcctgcct ggaaaggaat ggagatgatg ttcgatcatg
     3901 tcggcctgcg cgtcacggat ctcgaggcga gcgcacgcct gtacgcggcc tgcctcgcgc
     3961 cgctcggctt cgtgcccggc tccgccggcg aggactacgc cggcttcggg ccgccgcagg
     4021 ccagcgccct ctggctgcat cgcgatgcgt cggcccgcaa gggcgcgcac gtcgcgttcg
     4081 cggcgccgga ccgcgcggcc gtcgaccgct tccatgccga agggttgaag gcgggcgccc
     4141 gggacaacgg cgcgcccgga ctgcggcccg actactcacc gacctactac gcggcgtttc
     4201 tgctcgacct cgacggcaac aacatcgagg cggtctgcac gcgctgatca gccgccggcg
     4261 cgccgctgcg cctcgcgcat gtccgcggcg acgcgcgcgg cttgcggctg gatgcccgat
     4321 gccgcatccg cctcgagtcc gcgcaccacg cccgcctggt cgtcggcgga gcggttctgg
     4381 ctcagcttgc gctttccctc caggcgcgtg acctcgatct cgatgcccac gatcgcgcgc
     4441 aaggtggcgg ccaggtagtc ctcgggcgcg tcggagacgt gccagggcac ggggcggccg
     4501 ccttcgtggc ggtcggtcag gcgctcgagg aaggcgcgca gccaggccgg gtcgtcgatc
     4561 gcacgcagcc tgccgtgggc ctgcacgagg gagtagttcc aggtgggcac catcttgccg
     4621 tgctggaact tgctcgggta ccagttcggc gagacgtagg cctgcgcgcc gtggaagacc
     4681 gcgagcaccg cctggccgtc ggcctcgcgc cagagcgggt tggcacgcgc cacgtggccc
     4741 gcgaggcgcc agcccgtgtc cgtgcgctcg gccatcagcg ggatcacgtc ggccgcgagc
     4801 tcaggcccac ctcgcaccag ggtcgccagc ggggccgcgt gcatcagcgc tgcgagctgc
     4861 tcgggatcct gttcgacgaa agaggcgggc tggtacatgg tcgggtatcc gcggaagaag
     4921 ctgcctcaga cgggcacggt gtagttcagg gcgagccggc cgccgtcgac gtagacgacc
     4981 tccccggtga cgtagctcga cgcatcgctc agcagccagc agacgacgtc ggcgacttcc
     5041 tcgggctcgc ccaggcgctt catcggcgtg cggcccagga tgcgctggcg cgccgcgtcg
     5101 ctcgtgagca cggagttgcg cgccagctcg gtggagatcg tgcccggcgc gaccgcgttc
     5161 acgcgcacgc cgtggtcgat caggccgagc gccatcgcgc gcgtgagctg gttgatggcg
     5221 cccttgctcg cgttgtagct cgcgatggtg ggaatcgcca tcaggccgtt gaccgaggac
     5281 atgttgacga tcgcgccacg ccgttgcgcc gccatctggc gcgcgaccgc ctggccgacg
     5341 aggaacgagc ccttgaggtt gacgtcgatc acggcgtccc agtcggcttc ggtgatgtcg
     5401 aggaaatcgg cggcgcggaa gatgcccgcg ttgttgacga ggccgtcgac ggcgccgaat
     5461 tcgcgcacgg cggcggccag cgcggcatcg acctcggcct tggacgcgac gttgcaatgc
     5521 gcgtagatcg cgccgtggcc ggcggcgcgc agctcgtcgg cgagcgcctg gccggcggcg
     5581 tccgcgacgt cccacagcgc gacccgcgcg ccgtcgcgcg cgaggc
//
LOCUS       DASVLN010000409        16978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_342572, whole genome shotgun sequence.
ACCESSION   DASVLN010000409 DASVLN010000000
VERSION     DASVLN010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16978
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342572"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VF453_10085"
     CDS             complement(<1..360)
                     /locus_tag="VF453_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688047.1"
                     /translation="MNKLGLLSSALVGAVASARSMTPMAAIAAARLAGRRTPGRLAVL
                     DHPVARYGALAMGVGELVGDKMRTAPDRTVALGLLARVASAGIAGAALAPRGRERAGA
                     ALALATAVPLAYATLAGR"
     gene            complement(440..2731)
                     /gene="metE"
                     /locus_tag="VF453_10090"
     CDS             complement(440..2731)
                     /gene="metE"
                     /locus_tag="VF453_10090"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEX7688048.1"
                     /translation="MTTTHNLGFPRIGARRELKFGLESYWKGESSLDALKALGARLRA
                     RHWSQQSGLDLAPVGDFSFYDQMLDMSFTLGNLPERVRGFQGDALDNAFRVARGRTAP
                     TSDHAACCGGGVAAGEMTKWFDTNYHYIVPEFTADTRFALDASRLLEQLAEARAQGVR
                     AKPVIVGPVTYLALGKAKDGCDRLALLPRLVPVYAQLLDELARQGVEWVQVDEPILVT
                     ELAPDWLHAFNTAYHDLKAARVKLLLATYFGALEDNKHLAANLPVAGLHVDAVNGRED
                     VQPLVNLLSPMKVLSLGVINGRNVWKTDLAATLDWLEPLAGRLGERLWIAPSCSLLHV
                     PVDLAQEQALDADVKSWLAFALQKLEELQVLAGALRDGRASVAEALAANRAAIDARRR
                     SPRVKDPAVQAAVEALDADLGRRRSAYAQRAPRQAALLKLPPYPTTTIGSFPQTPDIR
                     RARGEFKAGRLDEAGYRAAMQAEIARSVREQEALGLDVLVHGEAERNDMVEYFGEQLG
                     GFAFSESGWVQSYGSRCVKPPILFGDISRPRAMTVEWIRYAQSLTPKPMKGMLTGPVT
                     ILNWSFVRDDQPRSATCRQLALAIRAEVLDLERAGVRVIQIDEAALREGLPLRRSQWR
                     RYLDWAVESFRIAANGVADETQIHTHMCYAEFNDIIASIVDMDADVITIETARSDMAL
                     LDAFDDFRYPNEIGPGVYDIHSPNIPSRDHIVQLMRKAAERIPAQRLWVNPDCGLKTR
                     QWGEVIPALANMVAAARVLREGA"
     gene            2834..3730
                     /locus_tag="VF453_10095"
     CDS             2834..3730
                     /locus_tag="VF453_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688049.1"
                     /translation="MLERSHLAIIREVDRQGSLTAAAEVLHLSQSALSHSMRKLEEQL
                     GTPVWLREGRSLRPTQAGQYLLAVAERVLPQLVLAETRIRQFAQGERGSLRIGMECHP
                     CYQWLLKVVSPYLARWPDVDVDVKQKFQFGGIGALFGFEIDLLVTPDPLHKPGLRFVP
                     VFDYEQVLVVARGHPLAGAAWVKPAQLAREVLITYPVPIERLDIYNQFLMPAGIAPRR
                     HKAIETTDIMLQMVASGRGVAALPRWLVDEYAAKMDVVPVRLGRHGIAKQIFLGAREA
                     DFEIDYLASFVELASGPGARGE"
     gene            complement(3746..5824)
                     /locus_tag="VF453_10100"
     CDS             complement(3746..5824)
                     /locus_tag="VF453_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7688050.1"
                     /translation="MLQDAALTAWDGRFDTFRLLANSLQAMIAYYDSAELGYGCRFAN
                     KAYAAAFGFTEASIVGKSFVQVIGEAAAREVQPSIDKAGRERAAVAYVRELAAADGSR
                     RWIEVNVLPHLDAAGDPLGAFVIVTDITGHRRATQALRESEERLAKFMQASAEGIAFH
                     RDGIVTDANPPLCELLGYTLEELRGRRAVDFVAPDMRAQVQSVLDDGRELAYESAVIR
                     RDGLRIPVELIGRTLTLGGEKTRMSVVRDLRDRHAAQARIHHLAHHDALTGLPNRMSF
                     MERLGHRLELARASGQSLALLFVDLDHFKRVNDSLGHLIGDKLLQTVAARISASLRSN
                     DIVARFGGDEFIVMLEDASPDDVGEVAHKLLRAIEVPVDAEGRDLSVTPSVGIAMFPR
                     DGATPTELIKNADTAMYIAKSRGRANCQFFDPAVASATYDALVLESELTRALQREEFV
                     LHFQPQVRARDGRLAGVEALIRWNHPERGLLRPDAFIALAEQQRVMLPISQWVLRSAA
                     RAALRWRSRGLIDVPIAVNLSTMQFRAKGFVDAVAKVLREEGVPGEWLELELTERMLM
                     DDIDEVKRTLQQLRALGMRVSVDDFGTGYSSLAHLKELPIDGMKIDRSFVRDLPHQRG
                     SVAIASAVVQMAQGLSLTVVAEGVENDGQRRFLQALGCELLQGELVSPPLPAAELARW
                     VAAHRVAAAG"
     gene            5990..6673
                     /locus_tag="VF453_10105"
     CDS             5990..6673
                     /locus_tag="VF453_10105"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006479900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7688051.1"
                     /translation="MHVVSEPAILYFGTPVVVISTLNEDGSANLAPMSSAFWLGWRCV
                     LGLGAGSQTARNLRRSRQCVLNLPSQDEVSHVDRLALTTGADPVPPIKAGRGYRHVRD
                     KFGISGFTPQPSQAVAPPRVRECPVQLEAVVEAIHGIADDDDAQRGRILNIEVRIVRV
                     HLDTAILMDGHPDRVDPDRWRPLVMSFQKFYGLGTAQLQPSALSSIAEAMYRSPDVDR
                     ARAASLATG"
     gene            7024..9906
                     /locus_tag="VF453_10110"
     CDS             7024..9906
                     /locus_tag="VF453_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7688052.1"
                     /translation="MKHRRNMLARAAALAIEGGVVACAALALPTAMAADGEGDVTTQR
                     VEITGSAIRRIESEGALPVTVISRDAIDRTGATSVTELIQDLPAMTGGNFQQSSSSVN
                     GNGNGTTTAAIHALDQKYTLVLLNGRRVAPFGGFGAAGADGAVNLQSLPLDAIERVEV
                     LTDGASALYGSDAIAGVVNFITKKNLTDGAVSFTGTRPTHAGGSKWSAGISKGFGDYD
                     RDGNNVLLSYSHDVQNQLMASQRAVSRRGGLIPVTINGQSAILDQTSSNSLPGTVILD
                     SGAAFSPYFNANGNCGTDPAVFASDGLCRTNYAATVQDIPSSKRDSVYVTDRFKLNAN
                     NTLFAEALWSKYDMTAAFAPSAQPMGLGTDDLPGQPLDILWQKYVVPYETANNDNSAG
                     GQMRYRSMEAGGRTDKWETVAHHFVLGAEGTLMDWDYNASVTLSSNTDQDKLAGGYLD
                     FNKFVNLISTGAYDPLVPVAGQSLDPAVLHGTFLKTKISQDVFSLHASRDLFELPGGQ
                     SSLALGLDVTRQHLQQQTTDLAQFGNGTAAEANATDYAVGGFYGYVPMEASRRNYGAF
                     AELLMPLARKTEVTASVRFDRYDRVHNDMPFTQVPNNLTPLPAEDEGNSFSHPTFKLS
                     FRAQPEDALLLRGSVGTGFKAPSVNQIAAPLAFSTNTSGSYVCPFPSNATCVANGTGT
                     QQWDLISAGNPASGSAGLKAETSRQWTFGGRFEPAKTMSFGLDYWAVKLSNQILSGMP
                     ENYAFANAAALSNLFVIPYQDPAGYQTVALIQKPFNAGKANYQGLDWEINLNTTTSFG
                     KVTADLSGTHMLKADYTLPGGTQSDLGRFGGDNNVVFRDIVRLMLGLQSGDFTHTLTV
                     NHKSGYADQPFTADSGNIVALAPDGSETSTGVAYNGHVSSYTLVDWQTRWQYSKNFRV
                     TFGINNLFDTKPPLSLKIVGGNQVGYDGRYADPTGRAIAVGASYRF"
     gene            complement(10257..11057)
                     /locus_tag="VF453_10115"
     CDS             complement(10257..11057)
                     /locus_tag="VF453_10115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7688053.1"
                     /translation="MNVLIVHAHPEPRSFNGAMKDVAVRELRGQGHAVTVSDLYQLGW
                     RAALGPDDFPEGRARADYLDLSREQEHAFANGSHCADVQAEQAKVAAADLVIFQFPMW
                     WFSMPAILKGWVDRVFSRGFAYSAGRKYDSGHFRGKRAMLCLTTGTASTLYEPNGIDG
                     DLHHVLWPIHNGILAYTGFTVLPPFAAWMPGRVTPEEREAYLAELARRLRNLDAEEPL
                     YFHPRSDYDETQRLKPGVLARSGVQWNPREGQSFEASASAHSQEGLRR"
     gene            complement(11116..11925)
                     /locus_tag="VF453_10120"
     CDS             complement(11116..11925)
                     /locus_tag="VF453_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7688054.1"
                     /translation="MMSSLRTRRSLLEAIALGVAFSVFPFGAHAMPSPNKVFTDPAVI
                     ELATACMDGKADRVASLLHQGADVHAVGKLGMTVPHFALLAQANAPQVMKLVLDAGGD
                     PVSVLDGGETLPMYAVSRDNADPEVVRVLLDHGISPNWHPPKEPFRNTSLLFGAISGH
                     NRPVVRLLLQRGADVNYDDGFSGSALHRAMTAYQFGIAADLVDAGIRLDLTTNSRPSA
                     KPTSKRKTALAQYCEDEGGKRGANPLPEIAAGWRELEAALARRGEKMPCTL"
     gene            complement(11922..13253)
                     /locus_tag="VF453_10125"
     CDS             complement(11922..13253)
                     /locus_tag="VF453_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688055.1"
                     /translation="MSGAKKVAAGALVGTEAAAGGATVVAGAAGYLGYQAAVKPVVGA
                     VGAVKDAAAAVKNRFSPQRPALVPCVRCQECMSAKQRAIRIEKRNELIAQAQASPDPA
                     VRAAGTELKSDMHAVELAALSEDTYKQYDPNATDKTPPSPWKAMTPEELEAADINPAD
                     MKAARAVVYKCPDDFPFDPKTVVAFQGTTADPDDILADHDQALGMKTAQYDAAQNLGK
                     QMKDNPAFEGAEVTGHSLGGGKAQAAGVVAGLKGQMFNSAGLNPRTLGLPADGLDGYA
                     SAFNQYRSDGGLSQGGGDPLTGTQNSPGFQKVLLGVVKGASGVIKANQWAGDQLGVSD
                     AVGAHVPQIGKDLIQRLTGITAQEAQKNFDFSGGKWYIPPAIGDVRNVASKNADGTPS
                     SLAAQHSIVNLHQGIESRKAADIQTMLDGTGNPAPVTDYISVAKAAPAPGK"
     gene            complement(13412..13774)
                     /locus_tag="VF453_10130"
     CDS             complement(13412..13774)
                     /locus_tag="VF453_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688056.1"
                     /translation="MISISQGQWDQLGRIDFESRMVGVLRKQFPEQTAAFTDRQLAEP
                     MPALVARAAGYGLVDEQSAAIFVLNTWLLGPDFDQRVPALRQILNDKQLSPGTKADAL
                     TNFSITVFHELEQTKGAA"
     gene            complement(14096..14827)
                     /locus_tag="VF453_10135"
     CDS             complement(14096..14827)
                     /locus_tag="VF453_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX7688057.1"
                     /translation="MTHRTTLIASSIPAPRLAPAVALLAGLACAGNALAQDAPLQNEL
                     RAGIYFVSYKTKADDLSGPFTPSGINLRVGSLTTPYFAYIRHVNADWDVELAGGVPPT
                     AKTYGKGPATVGSVPFNDQEIATAKWFSPTLLLEYKFLDPATVVRPYVGAGINYTRFY
                     RLRSTPAGDAANGGPTTAKLTSSVGPAVTVGAVWQVARQFDVVASYSAARVNSHYTSD
                     TAGVERETHIHFNPRTWVVSVGYAF"
     gene            complement(14841..16610)
                     /locus_tag="VF453_10140"
     CDS             complement(14841..16610)
                     /locus_tag="VF453_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7688058.1"
                     /translation="MRSSIAFGLAAVAAATLSACGGGGSGATVDNSTTPGTLMESPPI
                     RTASLNATDLTAQLGASDAGKALLAVAGAPTCGVDFHYFHYQTVGGKGEQTTASGAIM
                     APTGGSGCSGPRPILVYTHGTAVTKTYNLANILDSTNEAWQESAMIGAFFAAHGYIVV
                     ASNYAGYDSSPLPYHPYLNADQQSKDVINALTAARKALADGLPSGDTDNGKLFVTGYS
                     QGGHVAMATQRAMEAAGMTVTAAAPMSGPYAMLAFGDAAIAYSNPGLGGTIYYPMIVN
                     SYQQSYGNVYQNLTDVFSSTYATGIDTLIPGQYTYTTLVSSGKLPQFAVFDSTTPGQG
                     SEPSTGIPQLDAAMAAPDPATNPIGALGFGDPYLFTNQLRVAYALDAAQTPDGFIPTP
                     TTLVPPQTDPTLGIRLDLKKNDLRAGWSPHAPVMLCGGMNDPEVFYKVNTLAMKAIWS
                     QQVAGGLVNVVDVDPSTNGDPTKAGTLPTLIGTITAGVYASEPTAGAAKISADVQAAV
                     ATNAAFASYFSAPGVPNSPQGVMVLGLASVASQATTLYLGQGVTSPTELATDIGNAVV
                     AEYHFPLTQTACETAAQAFFAHF"
BASE COUNT         2519 a         5886 c         5985 g         2588 t
ORIGIN      
        1 gcggccggcg agggtcgcgt aggcgagcgg cacggcggtc gcgagcgcca gcgccgcccc
       61 cgcgcgctcg cggccgcgcg gagcgagcgc cgcgcccgcg atcccggcgc tcgccacgcg
      121 cgccagcagt ccgagcgcga cggtgcggtc gggcgccgtc ctcatcttgt cgccgaccag
      181 ctcgcctacg cccatggcca gcgcgccgta gcgcgcgacc ggatgatcga gcacggccag
      241 ccggccgggc gtccggcggc ctgcgagccg ggccgccgcg atcgcggcca tgggggtcat
      301 cgaacgcgcg ctcgcgacgg cgccgacgag ggcggaggac agcaggccga gcttgttcat
      361 cggagaaacc tgttcgtgtg gaggacggag gagtccgccc tgcgagcaag cgccgctccc
      421 gcgcacgacg gtcgcgcgct caggcaccct cgcgcaggac ccgtgcggcc gcgaccatgt
      481 tggccagcgc ggggatcacc tcgccccact ggcgcgtctt cagcccgcag tcgggattga
      541 cccacaggcg ctgcgcgggg atgcgctcgg cggccttcct catcagctgc acgatgtggt
      601 cgcggctcgg gatgttgggc gagtggatgt cgtagacccc ggggccgatc tcgttgggat
      661 agcggaagtc gtcgaacgcg tcgagcagcg ccatgtccga gcgcgcggtc tcgatggtga
      721 tgacgtccgc gtccatgtcg acgatcgacg cgatgatgtc gttgaactcc gcgtagcaca
      781 tgtgggtgtg gatctgcgtc tcgtcggcca cgccgttggc ggcgatgcga aacgactcga
      841 cggcccagtc gagatagcgc cgccactgcg accggcgcag cggcaggccc tcgcgcagcg
      901 cggcctcgtc gatctggatc acgcgcacgc cggccctctc gaggtccagc acctccgcgc
      961 ggatcgcgag cgcgagctgg cggcacgtcg cggagcgcgg ctggtcgtcg cgcacgaacg
     1021 accagttcag gatcgtcacc ggaccggtca gcatgccctt catcggcttg ggcgtcagcg
     1081 actgcgcgta ccggatccac tcgaccgtca tcgcccgcgg gcgcgagatg tcgccgaaga
     1141 ggatcggcgg cttcacgcag cgcgagccgt aggactgcac ccagccggac tcgctgaacg
     1201 cgaagccccc gagttgctcg ccgaagtatt cgaccatgtc gttgcgctct gcctcgccgt
     1261 gcaccagcac gtccaggccc agcgcctcct gctccctcac gctgcgcgcg atctcggcct
     1321 gcatcgcggc ccggtagccg gcctcgtcga ggcggccggc cttgaactcg ccgcgcgcgc
     1381 ggcggatgtc cggggtctgc gggaacgagc cgatcgtcgt cgtcggatac ggcggcagct
     1441 tcagcagcgc cgcctgccgg ggcgcgcgtt gggcgtaggc gctgcggcgg cgtccgaggt
     1501 cggcatccag cgcctcgacc gcggcctgga cggccggatc cttgacgcgc ggcgagcggc
     1561 ggcgggcgtc gatcgcggcg cggttggcgg ccagcgcctc cgcgaccgat gcgcggccgt
     1621 cgcgcagggc gccggccagc acctgcagct cctcgagctt ctgcagcgcg aaggcgagcc
     1681 acgacttcac gtcggcgtcc agcgcctgct cctgcgccag gtcgaccggc acgtgcagca
     1741 gcgagcacga cggcgcgatc cacaggcgct cgcccaagcg cccggcgagc ggctcgagcc
     1801 agtccagcgt cgccgccagg tcggtcttcc agacgttgcg gccattgatc acgcccagcg
     1861 acagcacctt catcggcgac agcaggttca cgagcggctg gacgtcctcg cgcccgttga
     1921 cggcatcgac gtgcaggccg gccaccggca ggttcgcggc gaggtgcttg ttgtcctcca
     1981 gcgcgccgaa gtaggtcgcc agcagcagct tcacgcgcgc ggccttgagg tcgtggtacg
     2041 ccgtgttgaa ggcgtgcagc cagtccggcg cgagctcggt gacgaggatg ggctcgtcga
     2101 cctgcaccca ctcgacgccc tgccgcgcca gctcgtccag cagctgcgcg tagaccggca
     2161 cgaggcgcgg cagcagcgcg aggcggtcgc agccgtcctt ggcctttccg agcgccaggt
     2221 aggtcaccgg cccgacgatc accggcttgg cccggacgcc ctgcgcgcgc gcctcggcga
     2281 gttgctccag caggcgcgag gcgtcgagcg cgaagcgcgt gtcggccgtg aactcgggga
     2341 cgatgtagtg gtagttcgtg tcgaaccact tcgtcatctc gccggccgcg acgccgccgc
     2401 cgcagcaggc ggcgtgatcc gacgtcggcg ccgtgcgccc gcgcgcgacg cggaaggcgt
     2461 tgtcgagggc gtcgccctgg aagccacgca cgcgttccgg caggttgccg agcgtgaagc
     2521 tcatgtcgag catctggtcg tagaacgaga agtcgccgac cggggcgagg tccagcccgg
     2581 actgctggct ccagtgacgt gcgcgcagcc gggcgccgag ggccttcagc gcgtcgagcg
     2641 aggactcgcc cttccagtag gactccaggc cgaacttgag ttcgcgccgg gcgccgatgc
     2701 ggggaaagcc gaggttgtgg gtcgtggtca tggatcgatc gttggcaagt ggtcgcgccg
     2761 atgatcggac ccggtgacca tgaagtaaaa tggtcattcc tcacgtatcg attcaattca
     2821 ttcatgcgtt cccatgctcg agcgtagcca cctcgcgatc atccgggagg tcgaccggca
     2881 gggctcgctg accgcggcgg ccgaggtgct gcacctgtcg cagtcggcgc tgagccactc
     2941 gatgcgcaag ctcgaggagc agctgggcac gccggtctgg ctgcgcgagg gccgcagcct
     3001 gcggccgacg caggcgggcc agtacctgct ggccgtcgcc gagcgcgtgc tgccgcagct
     3061 ggtgctggcc gagacgcgca tccgccagtt cgcccagggc gagcgcggca gcctgcgcat
     3121 cggcatggag tgccaccctt gctaccagtg gctgctgaag gtggtctcgc cctacctcgc
     3181 gcgctggccc gacgtcgacg tggacgtgaa gcagaagttc cagttcggcg gcatcggcgc
     3241 gctgttcggc ttcgagatcg acctgctcgt cacgcccgac ccgctgcaca agccgggcct
     3301 gcgcttcgtg cccgtcttcg actacgagca ggtgctggtc gtcgcgcgcg gccacccgct
     3361 cgcgggcgcc gcctgggtga agccggcgca gctcgcgcgc gaggtgctca tcacctatcc
     3421 cgtgccgatc gagcgcctgg acatctacaa ccagttcctc atgccggcgg gcatcgcgcc
     3481 gcgacgccac aaggccatcg agacgacgga catcatgctg cagatggtcg ccagcggccg
     3541 cggcgtcgcg gcgctgccgc gctggctggt cgacgagtac gccgcgaaga tggacgtggt
     3601 gccggtgcgc ctgggccggc acggcatcgc caagcagatc ttcctcggcg cgcgcgaggc
     3661 cgacttcgag atcgactacc tcgcgtcctt cgtggagctc gcgagcgggc cgggcgcgcg
     3721 cggcgagtga gcgcgggcgg ccgcttcagc ccgccgccgc cacccggtgc gcggcgaccc
     3781 accgcgccag ctcggctgcg ggcagcggcg ggctgacgag ctcgccctgc agcagctcgc
     3841 agccgagcgc ctgcaggaag cggcgctggc cgtcgttctc gacgccctcg gcgacgaccg
     3901 tcagcgacag gccctgggcc atctgcacca ccgcgctcgc gatggcgacg gagccgcgct
     3961 ggtgcgggag gtcgcgcacg aagctgcggt cgatcttcat gccgtcgatc ggcagctcct
     4021 tcaggtgggc cagcgaggag tagccggtgc cgaagtcgtc caccgacacg cgcatgccga
     4081 gcgcccgcag ctgctgcagc gtgcgcttca cctcgtcgat gtcgtccatc agcatccgct
     4141 cggtgagctc gagctccagc cactcgcccg gcacgccctc ctcgcgcagc accttggcca
     4201 cggcgtcgac gaagcccttg gcgcggaact gcatcgtcga caggttgacg gcgatcggca
     4261 cgtcgatcag cccgcgcgag cgccagcgca gggccgcgcg cgcggcgctg cgcagcaccc
     4321 attggctgat cggcagcatg acgcgctgct gctccgccag cgcgatgaag gcgtcggggc
     4381 gcagcaggcc gcgctcggga tggttccagc ggatcagcgc ctcgacgccg gcgagccggc
     4441 cgtccctcgc gcgcacttgg ggctggaaat gcagcacgaa ttcctcgcgc tggagcgcgc
     4501 gcgtcagctc gctctcgagc accagcgcgt cgtaggtggc gcttgccacg gccggatcga
     4561 agaactggca gttggcgcgg ccgcgcgact tggcgatgta catggccgtg tcggcgttct
     4621 tgatcagctc ggtgggcgtg gcgccgtcgc gcgggaacat cgcgatgccc accgatggcg
     4681 tcaccgacag gtcgcggccc tcggcgtcga ccggcacctc gatcgcccgc agcagcttgt
     4741 gggcgacctc gccgacgtcg tcgggcgagg cgtcctccag catcacgatg aactcgtcgc
     4801 cgccgaagcg cgcgacgatg tcgttggagc gcaggctcgc ggagatgcgg gcggccaccg
     4861 tctgcagcag cttgtcgccg atcaggtggc ccagcgagtc gttgacgcgc ttgaagtggt
     4921 cgaggtcgac gaacagcagc gccagcgact ggccggacgc ccgcgccagc tccaggcggt
     4981 ggcccaggcg ctccatgaag gacatgcggt tgggcaggcc ggtcagcgcg tcgtggtgcg
     5041 cgaggtggtg gatgcgcgcc tgcgcggcgt ggcggtcgcg caggtcgcgc accaccgaca
     5101 tgcgcgtctt ctcgccgccg agggtcagcg tgcggccgat gagctccacg gggatgcgca
     5161 gcccgtcgcg gcggatcacg gcgctctcgt aggccagctc gcgcccgtcg tcgagcaccg
     5221 actgcacctg cgcgcgcatg tcgggcgcga cgaagtcgac ggcgcgccgg ccgcgaagct
     5281 cttcgagcgt gtatcccagc agctcgcaca gcggcgggtt cgcgtcggtg acgatgccgt
     5341 cgcggtggaa cgcgatgccc tcggcgctcg cctgcatgaa cttggccagg cgctcctcgg
     5401 actcgcgcag cgcctgggtc gcgcggcgat ggccggtgat gtccgtgacg atcacgaacg
     5461 cgccgagcgg atcgcccgcc gcgtccaggt gcggcagcac gttgacctcg atccagcgcc
     5521 ggctgccgtc cgcggcggcg agctcgcgca cgtaggcgac ggccgcgcgc tcgcggccgg
     5581 ccttgtcgat ggacggctgc acctcccgcg ccgcggcctc gccgatgacc tgcacgaagc
     5641 tcttgcccac gatggacgcc tcggtgaacc cgaacgccgc cgcgtacgcc ttgttggcga
     5701 agcggcagcc gtagccgagt tccgccgagt cgtagtaggc gatcatcgcc tgcaggctgt
     5761 tggcgagcag gcggaaggtg tcgaacctgc cgtcccaggc ggtcaaagcg gcgtcttgga
     5821 gcatgcgcga tgataggaat cggcccgccg cctgggcagc cctaggtttt gggcgaccat
     5881 cgagcggagg tccatgcgca cccctgcaaa tcatgcgtcc gaatgcagcg agccgcgccg
     5941 catggcgatc gggacactgg cggccaatcc atcaccgcag ggccatccca tgcacgtcgt
     6001 cagcgaaccc gcgatcctct atttcggtac ccccgtggtc gtcatcagca ccctcaacga
     6061 ggacggcagc gccaacctcg cgccgatgtc gtcggcgttc tggctcggct ggcgctgcgt
     6121 gctcgggctg ggcgccgggt cgcagaccgc gcgcaacctg cggcgctcgc gccagtgcgt
     6181 gctgaacctg ccgtcgcagg acgaggtgtc gcacgtcgac cgcctcgcgc tcaccaccgg
     6241 cgccgacccg gtgccgccca tcaaggccgg gcgcggctat cgacatgtgc gcgacaagtt
     6301 cggcatcagc ggcttcacgc cgcagccctc gcaggcggtc gcgccgccgc gcgtgcgcga
     6361 atgcccggtg cagctcgagg cggtcgtcga ggcgatccac ggcatcgccg acgacgacga
     6421 cgcgcagcgc ggcaggatcc tcaacatcga ggtgcgcatc gtccgcgtcc acctcgacac
     6481 cgcgatcctg atggacggcc acccggatcg cgtcgacccc gaccgctggc gcccgctcgt
     6541 gatgagcttc cagaagttct acgggctggg cacggcgcaa ttgcagccat cggcgctctc
     6601 gagcatcgcc gaggcgatgt atcgcagccc ggacgtcgat cgggcgcggg cggcgagcct
     6661 cgcgacgggt tgatcgaacg agcgagctgc gatccgggtg cgtggactcc gcgagtccgg
     6721 agcccgcgcg ttatcgcaac tgccggaatc cccacaacag ggcgaaccct cgtgcatgtc
     6781 gcaccacgcg tgatcatgcg cacgaaggag gcgcgaaccc acgtcgtcgg acgtcgcatt
     6841 tgagccacaa ggccgatcga catcgccgtt tacccgatcc ccaaggacgg gctttaacgc
     6901 cacatttcag cttcgcaatt gttttcagct tcggcttcag cgcgaatgca atgcctgccg
     6961 atccgggccg ccttcgatcg ctgcaagccg ttcagggtca accaccagcc ttgggagaac
     7021 gaagtgaagc accgtcgaaa catgcttgcc cgcgccgccg cgctggccat cgagggaggc
     7081 gtggtcgcat gcgccgcgtt ggcgctgccg accgccatgg cggccgatgg ggaaggcgac
     7141 gtcaccacgc agcgcgtgga gatcacgggc tcggcgatcc gccgcatcga atccgaaggc
     7201 gcgctgcccg tcaccgtcat ctcgcgcgac gccatcgacc gcaccggcgc gacgtcggtc
     7261 accgagctga tccaggacct gccggcgatg accggcggca acttccagca gtcctcgagc
     7321 tcggtcaacg gcaacggcaa cggcacgacc acggcggcca tccatgccct cgaccagaag
     7381 tacacgctcg tgctgctcaa cggccggcgc gtcgcgccct tcggcggctt cggcgccgcc
     7441 ggcgcggacg gtgccgtgaa cctgcagagc ctgccgctgg acgccatcga gcgcgtcgag
     7501 gtgctcaccg acggcgcctc ggcgctctac ggctcggacg ccatcgccgg cgtggtgaac
     7561 ttcatcacca agaagaacct gacggacggc gccgtctcct tcaccggcac ccgtccgacg
     7621 catgccggcg gcagcaagtg gagcgccggc atcagcaagg gcttcggcga ctatgaccgc
     7681 gatggcaaca acgtcctgct gtcgtacagc cacgacgtgc agaaccagct gatggcgagc
     7741 cagcgcgccg tctcgcgacg tggcggcctg atcccggtga cgatcaacgg gcagtcggcg
     7801 atcctcgacc agacgtcgag caattcgctg ccgggcacgg tcatcctcga cagcggcgcc
     7861 gcgttcagcc cgtacttcaa cgccaacggc aactgcggca cggatcccgc cgtgttcgcg
     7921 tccgacggcc tgtgccgcac caactacgcg gccacggtgc aggacatccc gagctccaag
     7981 cgggacagcg tctacgtcac cgaccggttc aagctcaacg cgaacaacac gctgttcgcc
     8041 gaggcgctct ggtcgaagta cgacatgacg gccgcgttcg cgccgtccgc ccagccgatg
     8101 ggcctgggca cggacgacct gccgggccag ccgctggaca tcctgtggca gaagtacgtc
     8161 gtgccctacg agacggccaa caacgacaac agcgccggcg gccagatgcg ctatcgctcc
     8221 atggaagccg gcggccgcac ggacaagtgg gagacggtgg cccatcactt cgtcctgggc
     8281 gccgagggca cgctgatgga ctgggactac aacgccagcg tgaccctgtc ctcgaacacg
     8341 gaccaggaca agctcgccgg cggctacctc gacttcaaca agttcgtgaa cctgatctcg
     8401 accggcgcct acgaccccct ggtgccggtg gcgggccaga gcctcgaccc ggcggtgctg
     8461 cacggcacgt tcctgaagac gaagatctcg caggacgtgt tcagcctgca cgcgtcgcgc
     8521 gacctgttcg agctgccggg cggccagtcg tcgctggccc tgggcctcga cgtcacgcgc
     8581 cagcacctgc agcagcagac gacggacctc gcgcaattcg gcaacggcac cgcggccgag
     8641 gcgaacgcga ccgactacgc cgtcggcggc ttctacggct acgtgccgat ggaggcgagc
     8701 cgcaggaact acggcgcgtt cgccgagctg ctgatgccgc tggccaggaa gacggaagtc
     8761 acggcctccg tccgcttcga ccgctacgac cgcgtgcaca acgacatgcc cttcacgcag
     8821 gtccccaaca acctgacgcc gctgccggcg gaggacgaag gcaacagctt cagccacccg
     8881 accttcaagc tgagcttccg cgcccagccg gaggacgcgc tgctgctgcg cggctccgtg
     8941 ggcacgggct tcaaggcgcc gtcggtcaac cagatcgccg cgccgctggc cttcagcacc
     9001 aacacctcgg gctcgtacgt ctgcccgttc ccgagcaatg ccacctgcgt ggccaacggc
     9061 acgggcacgc agcagtggga cctcatctcg gccggcaacc cggccagcgg aagcgcgggc
     9121 ctgaaggccg agacctcgcg gcaatggacc ttcggcggcc gcttcgagcc cgccaagacg
     9181 atgtcgttcg gcctcgacta ctgggccgtc aagctcagca accagatcct gtcgggcatg
     9241 cccgagaact acgcgttcgc caatgccgcc gcgttgagca acctgttcgt catcccctac
     9301 caggatccgg cgggctacca gacggtcgcc ctgatccaga agccgttcaa cgccggcaag
     9361 gccaactacc agggcctcga ctgggaaatc aacctgaaca cgaccacctc gttcggcaag
     9421 gtgacggccg acctgagcgg cacccacatg ctcaaggcgg actacacgct gcccggcgga
     9481 acgcagtccg acctcgggcg cttcggcggc gacaacaacg tcgtcttccg cgacatcgtc
     9541 cgcctgatgc tgggcctgca gtccggcgat ttcacgcata cgctcaccgt gaaccacaag
     9601 tcgggctacg ccgaccagcc cttcaccgcg gactcgggca acatcgtggc gctggcgccc
     9661 gacggatcgg agacgtccac cggcgtggcg tacaacggtc acgtctcgtc ctacacgctc
     9721 gtcgattggc agacgcgctg gcaatactcg aagaacttcc gcgtcacctt cggcatcaac
     9781 aacctcttcg acacgaagcc gccgctgtcg ctgaagatcg tcggcggcaa ccaggtcggc
     9841 tacgacggcc gctatgccga cccgacggga cgcgccatcg cggtgggcgc cagctaccgc
     9901 ttctgatcgc gccgtggcgc gcgccggcac cccgaaggtt ccggcgcgcc cgtccgcgtg
     9961 aagccgcggc gatttccggg tcaccgcgcg ggcagcgggc ctgcacggat cgggggagcc
    10021 tggccgcctt catcgcgttc cgttccaagg gtcggtgaaa ctcagcttgg cgccttgcca
    10081 agcgaggatc gagcaattcg atccggccgg tcacgctcct cggcccagcg acggcgcaac
    10141 gtcgcgaaat caggaattga ggagcggcgg acgcgagcgc aaagcgtttt ggaagcccgc
    10201 tggatctcgc ccccaccatc ccgcggcgct caccggcctg aacgtcaagc cgacggctac
    10261 cggcgaaggc cttcttgcga gtgcgcagag gcggaggctt cgaacgactg gccttctcga
    10321 gggttccact gcacgcccga gcgcgccagg acgccgggct tcaggcgctg cgtctcgtcg
    10381 tagtcgctgc ggggatggaa gtacaacggc tcctctgcgt ccaggttgcg caggcgcctg
    10441 gcgagctctg ccaggtaggc ctcgcgctcc tcgggcgtga cacggcccgg catccaggcg
    10501 gcaaatggcg gcagcaccgt gaagccggtg tacgcgagga tgccgttgtg gatgggccag
    10561 aggacgtggt gcaagtcgcc gtcgatgccg ttcggctcat acagcgtcga agccgtgccg
    10621 gtggtcagac acagcatcgc gcgcttgccg cggaaatgtc cgctgtcgta cttgcggccc
    10681 gccgaatagg cgaagccgcg actgaacacc cggtcgaccc agcccttcag gatggcgggc
    10741 atcgagaacc accacatcgg gaactggaag atgaccaggt cggccgccgc caccttggcc
    10801 tgctccgcct gcacatcggc gcaatggctc ccgttcgcga acgcgtgctc ttgctcccgg
    10861 gacaggtcca agtagtcggc ccgggcccgt ccttcaggga agtcgtcggg gccgagcgcc
    10921 gcccgccagc cgagctgata gaggtcgctc accgtcacgg cgtgcccctg gccgcggagc
    10981 tcccgcaccg cgacgtcctt catggcgccg ttgaaggatc gcggctcggg atgcgcatgg
    11041 acgatcagga cgttcatgcc cccattctgc atgtcggcac gtcggcggcg ccggaccgcc
    11101 tggtgccgtg ccggatcaca gcgtgcaggg catcttctcg ccgcgccgcg ccagcgccgc
    11161 ctcgagctcg cgccagccgg cggcaatctc gggcagcggg ttggccccgc gcttgccgcc
    11221 ttcgtcttcg caatactggg ccagcgccgt cttgcgcttc gacgtcggct tcgcgctggg
    11281 acgactgttg gtcgtcaggt ccagacggat gcccgcatcc accagatcgg cggcgatgcc
    11341 gaattggtag gcggtcatgg cgcgatgcaa cgccgatccc gagaagccat cgtcgtaatt
    11401 gacgtccgca ccgcgttgca ggagcaagcg caccacggga cggttgtgcc ccgagatcgc
    11461 gccaaacagc agcgaagtgt tccggaacgg ctccttgggc gggtgccagt tggggctgat
    11521 gccgtgatcg agcaggacgc gcacgacttc cgggtccgcg ttgtcgcgcg agacggcgta
    11581 catgggcagc gtctcgccgc catcgagcac cgagaccggg tcgccgcccg cgtcgaggac
    11641 cagcttcatc acctggggcg cgttcgcctg cgccagcagg gcgaagtgcg ggacggtcat
    11701 gcccagcttg ccgaccgcat ggacgtccgc gccctggtgc agcagggaag cgacgcggtc
    11761 tgccttgccg tccatgcacg cggtagcgag ttcgatgacg gcggggtccg tgaagacctt
    11821 gttcggagag ggcatggcgt gagcaccgaa tggaaagacc gaaaaggcca cgccgagggc
    11881 gatcgcctcc agcaggctgc ggcgggttcg aaggctggac atcattttcc cggggcggga
    11941 gcggccttgg ccacggagat gtagtcggtg acgggcgccg ggttgcccgt gccgtccagc
    12001 atggtctgga tgtcggcggc cttgcgcgac tcgatgccct ggtgcaggtt gacgatgctg
    12061 tgctgcgccg ccaggctgga tggcgtaccg tcggcgttct tgctggccac gttgcgcacg
    12121 tcgccgatgg cgggcgggat gtaccacttg ccgccactga agtcgaagtt cttctgcgct
    12181 tcctgggcgg tgatgccggt cagtcgctgg atcaggtcct tgccgatctg cgggacgtgc
    12241 gcgcccacgg cgtcggacac tccgagctga tcgccggccc actggttggc cttgatcacg
    12301 ccggacgcgc ccttcaccac tccgagcagc accttctgga agcccggcga gttctgggtg
    12361 cctgtgagag gatcgccgcc cccttgggac aggccaccgt ccgaacgata ctggttgaac
    12421 gccgacgcat agccgtcgag gccgtccgcc ggcaggccca aggtccgcgg attcaggccg
    12481 gcggagttga acatctgacc cttcaggccc gccacgaccc ctgccgcctg ggccttgccg
    12541 ccgccaagcg aatggccggt aacctcggcg ccttcgaatg ccgggttgtc cttcatctgc
    12601 ttgcccaggt tctgggcggc gtcgtactgc gccgtcttca tccccaaggc ctggtcgtgg
    12661 tcggcgagga tgtcgtcggg gtctgccgtc gtgccctgga acgcgaccac cgtcttcggg
    12721 tcgaaaggaa agtcgtccgg gcacttgtag acgaccgccc gcgctgcctt catgtccgcg
    12781 ggattgatgt cggcagcctc gagttcctcc ggtgtcatcg ccttccaggg cgacggcggc
    12841 gtcttgtcgg tggcgttcgg atcgtattgc ttgtacgtgt cctccgacag cgccgccagc
    12901 tccaccgcat gcatgtcgct cttgagctcg gtaccggcgg cgcgcaccgc cggatcgggc
    12961 gaagcctgtg cctgcgcgat gagctcgttg cgcttttcga tgcggatggc gcgctgcttg
    13021 gcactcatgc attcctggca gcgcacgcat ggcaccagcg ccggccgctg cggcgagaac
    13081 cggttcttga ccgccgcggc cgcgtccttc acggcgccga cggcgccgac gaccggcttg
    13141 accgccgcct ggtagccgag gtagcccgca gcccccgcca cgacggtggc gccaccggca
    13201 gcggcttcgg tgccgacgag tgcgccggcc gccaccttct tggcccccga caccgtggcc
    13261 tcggccgcgg actgcgcgag ggatacgacc ttgtccttgg cggcggacgc cgccgagccg
    13321 atcgcgttca gcttgccgcc gatcgcctgc gcgcctgcgt tccagagatc gcgcagcgga
    13381 ttcccgtacg gcgtcggatc ttccccccag ctcatgccgc ccccttggtc tgctccagtt
    13441 cgtggaagac cgtgatcgag aagttcgtca gcgcgtcggc cttggtgccg ggcgacagct
    13501 gcttgtcgtt caggatctgc cgcagcgcgg ggacgcgctg gtcgaagtcc ggacccagca
    13561 gccaggtgtt cagcacgaag atggcggccg actgctcgtc gacgaggcca tagccggcgg
    13621 cgcgggcgac gagggcgggc atcggctcgg cgagctgccg atcggtgaat gcggcggtct
    13681 gctcggggaa ctgcttgcgc aggacgccga ccatccgcga ctcgaagtca atccgtccga
    13741 gctggtccca ctgaccctgg gagatagaaa tcatgttctt ctgcgatcac ggcgaccggg
    13801 tcaccgcgcc cgccgcgatg gactccggcc cgaccccgcc tgccgtggtc tcccagtatg
    13861 gcctgggcat acccgcgagc ctatcccccc ggcgggggtg ggacgcagcc agagggaacc
    13921 cgccagggat cgagtctcgt gcgcgatggc gctggtgggc ggctgggtcg cgaaatgcga
    13981 gggactcgcg ctcgcccccg gagacgccgg gcccgaggtc gccggccgcg gcaccgggac
    14041 gggcaccgcc gcgcccgatg tcgtcaatcc ccgccgagcc gggccgcgag tcgcgtcaga
    14101 acgcgtaccc caccgacacc acccacgtcc tcggattgaa gtggatgtgc gtctcccgct
    14161 cgacgcccgc ggtgtcggag gtgtagtggc tgttcacgcg cgcggcggag taggacgcga
    14221 cgacgtcgaa ctgcctggcg acctgccaga cggccccgac ggtgacggcc gggccgaccg
    14281 agctcgtgag cttggcggtc gtcgggccgc cgttggcggc gtcgccggcg ggcgtggagc
    14341 gcagccggta gaagcgggtg tagttgatgc cggcgccaac gtaggggcgc acgacggtgg
    14401 cggggtcgag gaacttgtac tcgagcagca gcgtcggcga gaaccacttc gccgtcgcga
    14461 tctcctggtc gttgaacggg acggagccga cggtcgccgg gcccttgccg taggtcttgg
    14521 cggtgggcgg cacgccgccg gccagctcga cgtcccagtc ggcgttgacg tggcggatgt
    14581 aggcgaagta aggcgtcgtg aggctgccca cgcgcaggtt gatgccgctg ggcgtgaacg
    14641 ggccgctgag gtcgtccgcc ttggtcttgt agctgacgaa gtagatgccg gcgcgcagct
    14701 cgttctggag cggcgcgtcc tgcgcaagcg cgttgccggc gcaggcgagg cccgccagca
    14761 gggcgaccgc cggggccagg cgcggcgcgg ggatggacga ggcgatgagg gtcgtgcggt
    14821 gcgtcatgcg tgtccccttg tcagaagtgg gcgaagaagg cctgggcagc cgtctcgcac
    14881 gccgtctgcg tgagcgggaa gtggtactcc gcgacgaccg cgttgccgat gtcggtcgcg
    14941 agctcggtcg ggctggtgac gccctggccg aggtacagcg tggtcgcctg cgaggcgacg
    15001 ctggccaggc ccagcaccat cacgccctgc ggcgagttgg gcacgccggg cgcgctgaag
    15061 tagctcgcga acgcggcgtt ggtcgcgacg gcggcctgca cgtcggcgct gatcttggcc
    15121 gcgccggcgg tcggctcgct cgcgtagacg ccggccgtga tggtgccgat cagcgtgggc
    15181 agcgtgccgg ccttggtcgg gtcgccgttg gtcgacgggt cgacgtcgac gacgttgacg
    15241 agcccccccg cgacctgctg cgaccagatc gccttcatcg ccagcgtgtt gaccttgtag
    15301 aacacctcgg ggtcgttcat gccgccgcac agcatcaccg gcgcatgagg cgaccagcct
    15361 gcgcgcaggt cgttcttctt caggtcgagg cggatgccca gcgtcgggtc ggtctgcggc
    15421 ggcaccagcg tcgtcggcgt cgggatgaag ccgtccggcg tctgcgcggc atcgagggcg
    15481 taggcgactc gcaactggtt ggtgaacagg tacggatccc cgaatcccag cgcgccgatc
    15541 gggttggtcg ccgggtcggg agccgccatg gcggcgtcga gctgcgggat gccggtgctc
    15601 ggctccgagc cctggccggg cgtcgtgctg tcgaagacgg cgaactgcgg cagcttgccg
    15661 gagctcacga gcgtcgtgta cgtgtactgg ccggggatca gcgtgtcgat gccggtggca
    15721 taggtcgagc tgaagacatc ggtcaggttc tggtagacgt tgccgtagga ctgctggtag
    15781 ctgttgacga tcatcgggta gtagatcgtc ccgcccaggc cggggttgct gtaggcgatg
    15841 gcggcgtcgc cgaacgcgag catcgcgtag ggccccgaca tcggcgccgc ggccgtgacc
    15901 gtcatgccgg cggcctccat cgcgcgctgc gtggccatcg ccacatggcc gccctgcgag
    15961 tagccggtga cgaacagctt gccgttgtcg gtgtcgcccg agggcaggcc gtcggcgagc
    16021 gccttgcgcg cggcggtcag ggcgttgatg acgtccttgg actgctggtc ggcgttgagg
    16081 taggggtggt atggcagggg cgacgaatcg tagccggcat agttggacgc gacgacgatg
    16141 tagccgtgcg cggcgaagaa cgcgccgatc atcgcgctct cctgccaggc ctcgttggtc
    16201 gagtcgagga tgttggcgag gttgtaggtc ttcgtgacgg ccgtgccgtg cgtgtagacg
    16261 aggatcggcc gcgggcccga gcagccggag ccgcccgtgg gcgccatgat cgcgccggac
    16321 gcggtggtct gctcgccctt gccgcccacc gtctggtagt ggaagtaatg gaagtcgacg
    16381 ccgcaggtcg gcgcgccggc gaccgcgagc agcgccttgc ctgcgtcgct ggcgcccagc
    16441 tgggccgtca ggtcggtcgc gttcagcgac gcggtgcgga tcggcggcga ctccatgagc
    16501 gtgccggggg tggtgctgtt gtcgacggtg gcgccggagc cgccgccgcc gcaggccgac
    16561 aaggtggccg ccgcgaccgc ggcaagcccg aatgcaatcg acgatcgcat ggtggtctcc
    16621 tgatgttcgt gacgcgatgc gcgcccgttc ccgtgcggcg cttcgtcgtc gccaatgggg
    16681 ttgccaggga cgatagaccg ggatcggcgg ttcgccggct gggggtttcc ctgtcacctg
    16741 cgtgtcatgg gaggcgggcg atccaccggg ctgtcgcggg gcttgcgcga aggaggcgtg
    16801 gctgcggggc ggcgcgcgag gaagtctcgg ggatcgcgat gccggcgcga tcccgccgcc
    16861 cagggcatcc ggcttgccga tgccgcctca tcgcaggagg acgccatgca cgatcacgag
    16921 tcgcccgacc atccgcccgg ccccgtggtc gtgctgaccg gcgccgccgg gggcatcg
//
LOCUS       DASVLN010000410        17761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_182627, whole genome shotgun sequence.
ACCESSION   DASVLN010000410 DASVLN010000000
VERSION     DASVLN010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 17761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17761
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182627"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2894
                     /locus_tag="VF453_10145"
     CDS             <1..2894
                     /locus_tag="VF453_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688059.1"
                     /translation="LAAQGRVEGGRIVGGVFIGRDGAPTALVVANPDAEPFASAVRAA
                     GGAPSAAATTSATHPTGVRIRDPKSWLHPLTLVTSVAMVHGLPLVLERMGATAFSGLL
                     DEAISGPEQTFVSQMRSLGTRLAYFLVSKAGMAGALQAAAKGDAGRADRLLVRVDRFG
                     HLAGMSRGELDNIAGVRKEIREFAEAVNAYKASIRKLGLHDEVGARFPVLFATEADVK
                     NGNVDAMSMRRMPEGEVIRHVLANKLPTLLPGVADADLLPRIVELVDDPARMAAIDRD
                     DPQLGAKLRIAKEGEEAYVQVRAALKDTNNAIDHQGIQKAAGSTMVMDTKWGTLFKLG
                     ALGFPMNSLVAAVGELHPTRLLDYREIIGNGPNALMQFKYLTTLNARRYAKSGSASDA
                     WIVKTLRNRQSALGTAQNRYGWLDAHVPDWRLLEDLRNYSTKRLEEVGDPLAAINDLL
                     DEFDQAYPDGPLDPAIADTGYADLMGRMRDMTNVAIGQRAGQLNTRIDYMALPSVARY
                     VMMGAYTVATHGDPAIAALQLFHGGVMNPLWLLAQQGHGSWRWFKYGADDASWKYAKL
                     GYSEPTRNKIRLAFLASALVTPTASLLYALLGGGSPASSNVAPGSTPSAGTGPTPSAA
                     NPSNTPTGIPAPSSPGGPAVPPGSQTTTLAPPIDPSGDAEADAAARSVSQSFAGGGDA
                     AAAARLAQVLRQAPMAERRAIMSAAMPVVRRIALDLGLNSRRTGAAWSAVPGVPLSAN
                     PGDVNPIDTRAEYLATLRDLAGSVQDTGDKPTAFVVGGDLLQLMPAGSVDDKANLLGL
                     LGDGATGLARGDTLLRDAVAGVLIDPWQAGRVGTELSVGMEQRDAAAGLLDPSSVARP
                     SLTVGDGYWTKDGVPYDHSVPGGKRHDGSVWSYVELNPDLYLTPLQQQGADRQARDGD
                     WSPYQVQQVKTDLGVRNLRGAYPDRDLDVVDVGERFAWGGDVATQKEGG"
     gene            complement(2895..3869)
                     /locus_tag="VF453_10150"
     CDS             complement(2895..3869)
                     /locus_tag="VF453_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7688060.1"
                     /translation="MMDLRQLRYFSVLAAQQHFGRAADVLHIAQPALTRQMKLLEEEL
                     GVMLFERHARGAALTEAGVQLQSRAEFLLRCAQQLRDDIATHQREPAGAVAIGLSPGM
                     APLLAAPLTLRIQQRYPSMRLRFVEGFAPVLHEAVLGGQIDVAVLNGAVRGIDLVMEP
                     LLREQMCLIGPAKDKRLKAASISVRHLDGLPLVMTGLAKSGVRLELEAAAARAGVALE
                     SVVEVESVDVARRLVLAGVGLTVHFAAVVHDDLQRKRLAAVPIEGLFLNRVIARAAGR
                     PPSRATEALTGVLREVVTEEVRSGRWPYATPDASDNASRSAPPASARR"
     gene            4019..4558
                     /locus_tag="VF453_10155"
     CDS             4019..4558
                     /locus_tag="VF453_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX7688061.1"
                     /translation="MDPTVQEMEATRIARWGRVTPYGETFIESRLPGMKKNLYKIINR
                     GVLENRGVQPAIPGEHRFGVTFIEVPAGQGASLHAHRTEEVFCPLNGRMEVIWGDRGQ
                     HSVMLEQWDVISCPIGVMRGFRNPNDHVLVIYSVVGGTDAEVGRIRWHDDVLTAAEGT
                     GMAYGADGYLATAADTASE"
     gene            4580..5491
                     /locus_tag="VF453_10160"
     CDS             4580..5491
                     /locus_tag="VF453_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEX7688062.1"
                     /translation="MTQAHATPRRRVEPAAIPPTPEALLDAVLKANAGAADPRARRVL
                     DAAIRHLHAFAAEVQLSYDELHRGADFLVRVGQATGPEKHEGILLADILGLATHVLLM
                     DAKAVLAAGGTEPALIGPFWRSGQPQRLNGESIVSAQTPGDALHVTGRVVSIDGAAIA
                     GARVETWQAAPNGLYENQDPHQDDMNLRAALVTDAEGRFWFDSVKPSGYGVPTDGPCG
                     ELLRLQGRSHMRPAHLHFVVIADGYKVLATQIFDARDPWAEQDAAFGAVGSLLRDFEP
                     DGQGGYALDLDFKLEPGVTVVPRPPLA"
     gene            5836..6474
                     /locus_tag="VF453_10165"
     CDS             5836..6474
                     /locus_tag="VF453_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7688063.1"
                     /translation="MTTAAIANPAMASWAALNERAMFAAGESVLINGANGVAGRLAIR
                     NAKLLGAGRIVVTAREESAREELARLGADAFISLSAAPDGVVDEVEREVARGIDVVLD
                     YLWGRPAECVVQAVMNGAKRSAPRRVRFVNIGSLAGMDMSLAAAALRSSRIELMGSGL
                     GSVSEERLASSAAAAVMALGAHLDGIPIRGVAIESLPAHWHAETTARLVFTF"
     gene            complement(6502..6957)
                     /locus_tag="VF453_10170"
     CDS             complement(6502..6957)
                     /locus_tag="VF453_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HEX7688064.1"
                     /translation="MSSNTQPHAPFTIIPERCVVVVNSELPPGRAGNAAAVLSLTVGQ
                     RHPQLVGAPLVDSNGVAVPGLIPIGISVLAGTPQELRELAQASAGTPIDVIRFPVEGQ
                     QTKNYTEFLDAIRRTRVADLDYVGIALVGERKPISKLVGKLPLFGQAAS"
     gene            complement(6961..7671)
                     /locus_tag="VF453_10175"
     CDS             complement(6961..7671)
                     /locus_tag="VF453_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7688065.1"
                     /translation="MSTTTRHQRLTPRAEQRILDLLSAARDVFAERGFERATTLEIAQ
                     RAGVSEPTVFTYFDSKRDLCVEVIRRWYGQITADIERDLPLFSGLPARLRFIVRRHLD
                     NLMGEGVGICALVLSEGRTADKTVTDVIAELKRAYVEPLMRSLRDAVAQGEIRDDMPL
                     RLLRDMVFGSMEHVLWGYVVSGVKPDIEVTSRQLTEMLCAAFAPASVPEQALRELRAD
                     VSLALRRCEQKLSHNHRD"
     gene            complement(8129..9493)
                     /locus_tag="VF453_10180"
     CDS             complement(8129..9493)
                     /locus_tag="VF453_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688066.1"
                     /translation="MINPRESISRRRAAVCAAYAACAGASLVGFMLPASAAAGRTGQD
                     KGHERISVSDYANQVFTATAKSPGDPNSHVATSFSSWRLAIQAALDEAYNRGGGTVVI
                     DGGSAPFSHPTLLIDDYLRIRDNTTVVFEAEVVLGDYTKLGSVIDMNGDNIVVNNPRI
                     NASGLFAGASGHNGLSGGTCSNVKVNGGTIRNCARGNAGGAFGGKGFQFEGTFHNVEV
                     SGTTLDTCFMALSTNHDFSSTKPSGPLVFKDITAKSCAILLFVAQTNGHDTTGQAHSV
                     ALTGFRAKDCGTFEGLMQFSRASNVQVSDGIVSNATVIDSLVRGNHSSCRFENIRFTG
                     DCSTALINLDPSTYARDASYRMENNSYSIEQDGTTPYIAYASPTTPNRTLADSRIRAR
                     LTNDVAKKIVGDELRNAYCVVEAQQGDTCIEVKTADLYLRGLARFASFRGKDEGSCRR
                     RRPA"
     gene            10136..11101
                     /locus_tag="VF453_10185"
     CDS             10136..11101
                     /locus_tag="VF453_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7688067.1"
                     /translation="MNRLRRAALLLATGQRFLLVDRPRQLPRRVLWYYDWETLGDAVM
                     DLSQRVLIDASIELDLCMPKGPIELFEHDARFRRVHRHLSDCKGPYDLIVTQWVTTEL
                     IRRKARHFPLVPWLSVMCHERGELFCRATLSFARLRPLFGPDCSSTAVPPSLDLASCA
                     ACGFEPVDIVVAVGGRDPRRTYSRWPEVVETLVRHWPPQRGRPTIALVGSGAAAKVAA
                     DEIGRLGLAARIDVHLDLPGPLQAAQLIAAGQVFVGCDGGLMHVAAALGKPGAALFCE
                     ILPRWRLHATSGIVGLFDPHGMDRIEAAAVASATLRAAGLDAPLS"
     gene            complement(11181..12128)
                     /locus_tag="VF453_10190"
     CDS             complement(11181..12128)
                     /locus_tag="VF453_10190"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX7688068.1"
                     /translation="MGEGALRILTMAAAATVHLGLALVVGAVASQGWLWRQPSAWRDR
                     VVEQALGMRRLGFALGLAGTLAAPWFEAALMTGQPLLQAGPAVGTLLAHSHFGRATSI
                     GIVAWLLAAATLIPRSSTRDTAARRALALGALAVFIATRTAVSHAGSDGDLTRDVAVG
                     WLHLLLVYLWVGIVAAGTRLELPAACAPSAERQAATRWVSRMSSTATVSLVGIGASGL
                     FKVWRAFEPSASARQFIDSDYGHTLLVKLALVAVAVALGGANRFIVLPRLFAELAGVE
                     NGGGWRRGLTRILHVEAATLLLVLVAATVLGGTEPPGEG"
     gene            complement(12128..12478)
                     /locus_tag="VF453_10195"
     CDS             complement(12128..12478)
                     /locus_tag="VF453_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEX7688069.1"
                     /translation="MKKPLAFIALTLASALALAHAKLQASTPASGAAVSPASAELRLR
                     YNEPVEVAMSTVRITGPGDVAVATDKVLADPSDDKTLVQPLPRLAAGEYRVQWTTMGH
                     DGHHTKGEIRFAVK"
     gene            complement(12508..13878)
                     /locus_tag="VF453_10200"
     CDS             complement(12508..13878)
                     /locus_tag="VF453_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEX7688070.1"
                     /translation="MTHRRSFFKAAGATMLGAAAVSRAGAAMLPEAASQTSPTMQPPP
                     SPPTGRPFHPVVTLNGWSLPWRMKDGVKEFHLVAEPVVRELAPGMKANLWGYNGSSPG
                     PTIEAVEGDRVRIFVTNKLPEHTTVHWHGVLVPNGMDGVGGLTQPHIPPGKTFVYEFV
                     LQRPGTHMYHPHADEMVQMAMGMMGLFIIHPRDVRQLRVDRDFAFVMASYAIDPGAAT
                     PRTSEMTDFNLWAWNSRVFPGIDPLVARTGDRVRVRVGNLTMTNHPIHMHGPHFEVTG
                     TDGGWIPKSARWPEVTTDVAVGQMRAIEFDAVAGDWAIHCHKSHHTMGPMSHTAANMI
                     GVDQRAAAEKVARLVPGYMAMGSSGMSDMAEMRMPLPDNTLPMMSGEGPYGGLEMGGM
                     FSVVKVRDGVEPGDYRDPGWFRHPQGTVASEYTADDAAPAPQASQPASDAATLHVHKP
                     TGHEGH"
     gene            complement(13887..15161)
                     /locus_tag="VF453_10205"
     CDS             complement(13887..15161)
                     /locus_tag="VF453_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7688071.1"
                     /translation="MSRVSRLTQERTGHPVALQRDRQDTQAARSRVAELLRAPLSADG
                     AVEVALLNNPALQQQLGNVGISEADLVQAGRLRNPAISLGRLAGGDALEIDRSVMFDV
                     LGLLTLPGRSRIARDRFDQAQLQAAADAVAVAQQARQAYFDAVAAQQLDAYARQVTEA
                     ADAADELAKGLLRAGNVSKLQQMREQAFHADAMAQAAHSHQQALSARERLVRALGLGD
                     QQALLRLPERLPDLPQDVAAPEDAEQAALDKRLDVLMARRETEATARQLGLTRVTGFV
                     NALDAGWQDKRQRGAAPERGGQVQFELPIFDFGDADRARAQAVYLQAVNRAAATAVDA
                     RSEVREAYAGYRGAFDLARHYRDEVVPLHQRISEETLLRYNGMLADVFDLLADAREQV
                     ASVTASVEATRDFWLADARLQEALAGASPTHP"
     gene            complement(15398..15742)
                     /locus_tag="VF453_10210"
     CDS             complement(15398..15742)
                     /locus_tag="VF453_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688072.1"
                     /translation="MAILLPARGAIAAASPCEGLAHQAPAQRMHTHHPMHAAVEPDAA
                     SETGHGAAHHDHAGVDKCNLCASCCSATPMLMTFSPTVAALGDQVATFPLLQASAPTF
                     LSEGLERPPRSI"
     gene            complement(15813..17192)
                     /locus_tag="VF453_10215"
     CDS             complement(15813..17192)
                     /locus_tag="VF453_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7688073.1"
                     /translation="MSPTPESSSTLRAQLELTRVRLTGLVGRGLPISIGLIVLVSLAV
                     GALAFIFLNSAPPTTLTMTSGPEGSSFRLVAEQYRKILAREGVTLRIVTSRGSRDNLA
                     RLADPKQDVDVGFVVGGEAPEGDAGRRLMSLGSVSYQPLMIFYRGRPKTLISEFKGER
                     IDIGQDGSGASALSQRLFEANGIKPGDGTRIGHAAPEASVKELLGGRIDALFAMSDST
                     PTGLMRQLLRDPDIHLYSFAQADGYTHRITYLNRLVLPRGAIDFGEDVPAEDVKLVAP
                     TVELVARDSLHPALSDLLLEAAREVHGRAGLYKKSGEFPAPIQHELPISADASRYYAS
                     GRGFFYRTFPFWIASLIERVLAVVVPMTIVLIPGIKIAPAVFRWRIESRIYRWYAVLQ
                     RIDRDSYEQPLDARRCDELLHRLAHVESTVARLVVPPAYGDVLYALRGHIVAVRESLM
                     ERREPVGQG"
     gene            17389..>17761
                     /locus_tag="VF453_10220"
     CDS             17389..>17761
                     /locus_tag="VF453_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688074.1"
                     /translation="MGAFWSNFRRGVAELVRAVSACVFPVFSLLAWFGILLAGQGEDV
                     VRRSVERAALDDSASELIALAIASVLLGLSVWYAMRSLLSVQRPGLPPPNPGPHGPTA
                     WLPRILGALAVAWPGWVAHDAV"
BASE COUNT         2610 a         6186 c         6267 g         2698 t
ORIGIN      
        1 cgcttgccgc tcagggccgc gtcgagggcg gccgcatcgt cggcggcgtc ttcatcggcc
       61 gcgacggcgc gccgaccgcc ctcgtcgtcg cgaaccccga cgcggaaccc ttcgcgagcg
      121 cggtgcgtgc ggcgggcggc gcgccttccg ctgcagcgac cacctccgcg acgcacccga
      181 ccggcgtgcg catccgcgat ccgaagtcct ggctgcaccc gctcacgctc gtgacgtccg
      241 tcgccatggt gcacggcctg cctctggtgc tggaacgcat gggtgccacg gcgttctccg
      301 ggcttctcga cgaggcgatc agcgggcccg agcagacctt cgtgagccag atgcgctcgc
      361 tgggcacgcg gctcgcatac ttcctcgtct cgaaggccgg catggccggc gcgttgcagg
      421 cggcggccaa gggcgacgcg ggccgtgcgg accggctgct cgtgcgagtc gaccggttcg
      481 gccacctcgc gggcatgtcg cgcggcgagc tggacaacat cgccggcgtc cgcaaggaga
      541 tccgcgagtt cgccgaagcg gtgaacgcct acaaggccag catccgcaag ctcggcctgc
      601 atgacgaggt cggagcgcgg ttcccggtgc tgttcgcgac cgaggccgac gtgaagaacg
      661 gcaacgtcga cgccatgagc atgcgcagga tgcccgaggg cgaggtgatc cgccacgtcc
      721 tcgccaacaa gctgccgacg ctgctgccgg gcgtcgccga tgccgacctg ctgccgcgca
      781 tcgtcgagct cgtcgacgat ccggcccgga tggcggcgat cgaccgcgac gatccgcagc
      841 tgggggccaa gctgcgcatc gccaaggaag gcgaggaggc ctacgtccag gtgcgcgcgg
      901 cgctcaagga caccaacaac gcgatcgacc accagggcat ccagaaggcc gcgggcagca
      961 ccatggtcat ggatacaaag tggggtacct tgttcaagct gggcgcgctc ggcttcccga
     1021 tgaactcgct ggtcgccgcg gtcggcgagc tgcatcccac gcggctcctg gactaccgcg
     1081 agatcatcgg caacggcccg aacgcgctga tgcagttcaa gtacctgacg accctgaatg
     1141 cgcgccggta cgccaagagc ggcagcgcca gcgatgcgtg gatcgtcaag acgctgcgca
     1201 accggcaatc cgcgctgggc accgcgcaga accgctacgg ctggctggac gcccacgtgc
     1261 cggactggcg cctgctggag gacctgcgca actattcgac caagcgcctc gaggaggtcg
     1321 gcgacccgct cgccgccatc aacgacctgc tggacgaatt cgaccaggcg tatcccgacg
     1381 ggccgctgga tccggcgatc gccgacaccg gctacgcgga tctcatgggc cggatgcgcg
     1441 acatgacgaa cgtggccatc ggccagcgcg ccggccagct gaacacccgc atcgactaca
     1501 tggcgctgcc cagcgtcgcg cgctacgtga tgatgggcgc gtacacggtc gccacgcacg
     1561 gcgatcccgc gatcgcggcg ctccagctgt tccacggcgg cgtgatgaac ccgttgtggc
     1621 tgctcgccca gcaggggcac ggcagctggc ggtggttcaa gtacggcgcc gacgacgcga
     1681 gctggaaata cgcgaagctg ggctactcgg aaccgacccg caacaagatc cgcctcgcgt
     1741 tcctcgccag cgcgctggtc acgccgacgg cctcgctgct gtacgccttg ctgggcggcg
     1801 ggtcgccggc ctcctcgaac gtggcgcccg gctcgacgcc ttcggcgggg accggcccga
     1861 cgccctccgc cgcgaatccg tcgaatacac cgacgggtat tcccgcgccg tcatcgcccg
     1921 gcgggccggc cgtgcccccc ggatcgcaga cgacgacgct ggcaccgccg atcgacccga
     1981 gcggcgacgc ggaggccgat gccgcggcgc gcagcgtctc gcagtccttc gccggcggcg
     2041 gcgacgcagc ggccgcggcc cggctcgcgc aggtgctgcg gcaggcgccg atggccgagc
     2101 gccgggccat catgtcggcg gccatgccgg tcgtccgccg gatcgcgctc gacctcggcc
     2161 tgaactcgcg ccgcaccggc gccgcctgga gcgccgtgcc cggcgtgccg ctcagcgcga
     2221 acccgggcga cgtcaacccg atcgataccc gggcggagta cctggcgacg ctccgcgacc
     2281 tcgccggcag cgtgcaggac accggcgaca agcccaccgc gttcgtggtg ggcggcgacc
     2341 tcctgcagct gatgccggcc ggcagcgtcg acgacaaggc gaacctgctc gggctgctcg
     2401 gcgacggcgc gaccggcctg gcccgcggcg acacgctgct gcgcgatgcc gtggccgggg
     2461 tgctgatcga cccgtggcaa gccgggcgcg tcggcaccga gctttccgtg ggcatggagc
     2521 aacgcgacgc cgccgccggc ctgctggatc cgtcgtccgt ggcgcggccc tcgctcacgg
     2581 tcggcgacgg ctactggacg aaggacggcg tgccttacga ccattcggtg ccgggcggca
     2641 agcgtcacga cggctcggta tggagctacg tggaactcaa ccccgacctc tacctgacgc
     2701 cgctccagca acagggcgcc gatcggcagg cgcgcgacgg cgactggagc ccgtaccagg
     2761 tgcaacaggt caagaccgac ctcggcgtgc gcaacctgcg cggggcctat ccggatcgcg
     2821 acctcgacgt ggtggacgtc ggcgagcgct tcgcctgggg cggcgacgtc gcgacgcaga
     2881 aggagggcgg ctagtcaacg ccgtgcgctc gccggaggcg ccgatcgaga ggcgttgtcc
     2941 gaggcgtccg gcgtggcgta cggccagcgg cccgatcgca cctcctcggt caccacctcc
     3001 cgcagcacgc cggtcagggc ctcggtggcg cgcgaaggcg ggcgtcctgc ggcgcgggcg
     3061 atcacacgat tcaggaacag gccttcgatc ggcaccgccg caagccgctt gcgctgcagg
     3121 tcgtcgtgca cgacggccgc gaagtgcacc gtcaacccca cgccggccag caccaggcgc
     3181 cttgcgacgt ccacggactc gacttcgacc accgactcga gcgcgacgcc tgcgcgcgcg
     3241 gccgcggcct cgagctcgag ccgaacgccg gacttcgcca ggcccgtcat cacgagcgga
     3301 agtccgtcca ggtgccgcac gctgatggac gccgccttca ggcgcttgtc cttcgccggt
     3361 ccgatcaggc acatctgctc gcgcagcagc ggctccatca cgaggtcgat cccccggacc
     3421 gcaccgttga gcaccgccac atcgatctgc ccgcccagca cggcctcatg gagtaccggc
     3481 gcgaagccct cgacgaagcg cagtcgcatg gacgggtagc gctgctggat gcgcagcgtc
     3541 aacggcgccg cgagcagggg cgccatcccc ggagacagcc cgatcgcgac ggcacccgcg
     3601 ggctcgcgct ggtgcgtggc gatgtcgtcg cgcagctgct gggcgcagcg caggaggaat
     3661 tcggcgcgag attgcaactg cacgcccgcc tcggtcaggg ccgcgccgcg cgcgtgacgt
     3721 tcgaacagca tcacgcccag ttcctcctcg aggagcttca tctggcgcgt gagtgccggc
     3781 tgggcgatgt gcagcacgtc ggcggcgcgg ccgaagtgct gctgcgccgc caggacgctg
     3841 aagtaccgta actgcctgag atccatcatt tgccgcaatg agcgtcgata ccggattgtt
     3901 atcgaagcgg tcgaaaaatg gtaatggatg ctccagaggg aggctcctaa agtccgtttc
     3961 gtcgatacag gttggcgtct ttttcgtcat ccgtcattca tcaccgaaag gaatcaacat
     4021 ggatccgacc gtccaggaaa tggaagcgac ccgcatcgcg cgttgggggc gggtgacccc
     4081 ttacggcgag accttcatcg agagccggct gcccggcatg aagaagaatc tctacaagat
     4141 catcaatcgc ggcgtcctcg agaacagggg cgtccagccg gccatcccgg gcgaacatcg
     4201 cttcggcgtc accttcatcg aggttcccgc gggccagggc gccagcctcc acgcgcaccg
     4261 gaccgaggag gtgttctgcc cgctcaacgg ccgcatggaa gtgatctggg gcgaccgcgg
     4321 acagcattcc gtgatgctgg agcaatggga cgtcatctcg tgcccgatcg gcgtgatgcg
     4381 cggcttccgc aatcccaacg atcatgtgct ggtcatctac tcggtggtcg gcggcaccga
     4441 tgccgaggtg ggccgcatcc gatggcacga cgatgtgctg acggccgccg aagggacggg
     4501 catggcctac ggcgccgacg gctacctcgc gacggccgcc gacacggcga gcgagtaacc
     4561 ggccggccgc gagatggtca tgacccaagc ccatgccacc cctcgtcgcc gcgtggagcc
     4621 cgcggcgata ccccccacgc ccgaggcctt gctcgacgcg gtgctgaagg ccaacgccgg
     4681 cgccgccgac ccgcgcgccc gacgcgtgct cgatgccgcc atccgccatc tccacgcctt
     4741 tgcggccgag gtgcagctct cctacgacga actccatcgc ggcgcggatt tcctcgtgcg
     4801 cgtcggccag gccaccgggc cggaaaagca cgagggcatc ttgctggccg acatcctcgg
     4861 cctggccacg cacgtgctgc tgatggacgc gaaggccgtg ctcgccgcag gcgggacgga
     4921 gcccgcgttg atcgggccgt tctggcgctc gggccagccg cagcgtctca acggcgagtc
     4981 catcgtctcc gcgcagacgc cgggcgatgc gttgcacgtc accggccgcg tcgtgtcgat
     5041 cgacggcgcg gcgatcgccg gcgcgcgcgt cgagacatgg caggccgcgc cgaacggcct
     5101 gtacgagaac caggatcccc accaggacga catgaacctg cgcgcggccc tcgtcaccga
     5161 cgccgaaggc cgcttctggt tcgacagcgt caagccgtcg ggatacggcg tgcccacgga
     5221 cgggccctgc ggcgaactgc tgcggctgca gggccggagc cacatgcgtc ccgcgcacct
     5281 gcacttcgtc gtgatcgccg acggctacaa ggtgctcgcg acgcagatct tcgacgcgcg
     5341 cgatccctgg gccgagcagg acgccgcgtt cggcgccgtc ggatcgttgc tcagggactt
     5401 cgagcccgac gggcagggcg gctacgcgct cgacctcgac ttcaagctcg aaccgggcgt
     5461 caccgtcgtc ccgcgcccgc cgctcgcctg agcgtccgcg aaccctaacg caatgccatc
     5521 atgaaagctg ctgtcgtcca gagtttcgat cgccttccgg cgctcgtcga atttcccatg
     5581 cccgaagcgg cgccgcacga agtcctggtg cgcgtcgaag cggccgcgct gagccagctc
     5641 gcgcgcgggc aggccgccgg tcgccactac agcgcaccgt cgctcccgtt cgttcccggt
     5701 gccgacggcg tcggccgcct cgcgggcggc gagcgggcct acttcgcgtt ccctcgcagg
     5761 ccctggggcg cgatgggcga gtggacgacc gtcgatcgcc gcaacgtgat cccgataccc
     5821 gagtcgctcg atcccgtgac gacggccgcg atcgccaatc ccgcgatggc gtcctgggcg
     5881 gcgctcaacg agcgcgcgat gttcgcggcg ggggagagcg tgctgatcaa cggcgcgaac
     5941 ggggtcgccg gccgcctcgc gatccgcaac gcgaagctgc tcggcgcggg acggatcgtc
     6001 gtgaccgcac gcgaagagtc ggcgcgtgag gaactcgccc ggctcggtgc cgacgcgttc
     6061 atctcgctca gtgcggcgcc cgacggcgtc gtcgacgagg tcgagcgcga agtcgcgcgg
     6121 gggatcgatg tcgtgctcga ctacctgtgg ggccggccgg ccgaatgcgt cgtccaggcc
     6181 gtcatgaacg gcgcgaagcg ttcggcgccg cgccgcgtcc gcttcgtgaa catcggctcg
     6241 ctggccggca tggacatgtc actggccgcg gccgcgctgc gaagcagccg gatcgaattg
     6301 atgggaagcg gcctgggaag cgtgtcggag gaacggctgg cgtcgtcggc ggccgcggcc
     6361 gtcatggcgt tgggcgcgca cctggacggc atcccgattc gcggcgtcgc gatcgagtcg
     6421 ctgccggcgc attggcatgc ggaaacgacg gcgcggctgg tgttcacgtt ctgagatccc
     6481 tgccgcgcgc tgccgacgtc gtcatgacgc cgcttgcccg aacagcggca gcttgccgac
     6541 cagcttggag atcggcttgc gttcgccgac gagcgcgatt ccgacatagt cgagatccgc
     6601 gactcgcgtc cgcctgatcg cgtccaggaa ctcggtgtag ttcttggtct gctgtccctc
     6661 gacgggaaac cggatcacgt cgatgggggt gccggcgctt gcctgggcga gttcccgcaa
     6721 ttcctgcggc gttcccgcga gcacggagat gccgatcggg atcaggcccg gcacggccac
     6781 gccgttcgag tcgaccagcg gcgcgccgac cagctgcggg tgccgctggc cgaccgtgag
     6841 cgacagcacg gccgccgcat tgccggcccg gccgggcggc agttcgctgt tgacgacgac
     6901 gacgcaacgc tcgggaatga tggtgaaggg cgcgtggggc tgggtgttgg atgacatggt
     6961 tcaatcccga tggttgtggc tcagcttctg ctcgcaacgc ctcagggcga gcgagacgtc
     7021 cgcacgcagc tcacgcagcg cctgctccgg gaccgacgca ggcgcgaagg cggcgcagag
     7081 catctccgtg agctgccggc tcgtgacctc gatgtccggc ttcacgcctg agacgacgta
     7141 gccccagagg acatgctcca tcgacccgaa gaccatgtcc cgcagcagcc gcaggggcat
     7201 gtcgtcgcgg atctcgcctt gcgcgaccgc gtccctgagc gagcgcatca ggggctcgac
     7261 gtaggcgcgc ttcagctcgg cgatcacgtc ggtgacggtc ttgtcggcgg tgcgtccctc
     7321 gctcagcacg agcgcgcaga tgcccacgcc ttcgcccatc aggttgtcga ggtgtctgcg
     7381 cacgatgaag cgcaggcgcg ccggcaggcc gctgaaaagg ggcaggtcac gctcgatgtc
     7441 ggcggtgatc tggccatacc agcgccggat cacctcgacg cacaagtctc gcttgctgtc
     7501 gaagtaggtg aagacggtgg gctcggagac gccggcgcgc tgcgcgatct ccagcgtcgt
     7561 cgcccgctcg aaaccgcgtt cggcgaagac gtctcgagcg gcactcagca aatcgaggat
     7621 gcgttgctcg gcgcggggtg tgaggcgctg gtggcgcgtt gtggtggaca tgggatgctc
     7681 aagcggagtt cgatttgagc atcactcact gctagacgca agtgaccgtc ggaattctct
     7741 ctgcaggatc agcgcgagtg cggctcaact cctggacgcg gtcggcggcc ggcaaggcca
     7801 ccgccgacgc tctttcatgg tgatattact gcgtataata tgcgttatgt caaataacaa
     7861 tgaccccgaa gaagccgccc gccggtgtcg cgtcccatct tcgatgtcca aaactcacga
     7921 tccgtgtcca cgccacctcg cgctcgcgtt tggcatcgac cgacctttcg gagctcagcg
     7981 ccctggagat cgtcgccccg tggataggcc ttcagcatcg cagccccggc gcgccggaat
     8041 cgaatgcgtc gccgtcagcc cgacttgagg ggacgggtgc gtccacagag cgcgatcacc
     8101 tgccacaccc gatgccaggg cgtcagcgct atgccggccg acgcctccgg cacgaaccct
     8161 cgtccttgcc gcgaaagctc gcgaaccgag cgagtccgcg caggtacaaa tccgccgtct
     8221 tgacctcaat gcaggtgtcg ccctgctgtg cctcgaccac gcagtacgcg tttcgcagtt
     8281 cgtcgccgac gatcttcttc gccacatcgt tcgtgagtcg ggcccggatc cgggaatccg
     8341 cgagcgtgcg gttcggcgtg gtggggctcg cgtaagcgat gtagggcgtc gtaccgtcct
     8401 gctcgatgga atagctgttg ttttccatcc gatagctcgc gtcccgcgcg taggtgctgg
     8461 gatccagatt gatcagtgca gtggaacagt cacccgtgaa cctgatgttc tcgaagcggc
     8521 aggacgaatg gttgccgcga acgagcgaat cgatcaccgt cgcattgctg acgatcccgt
     8581 cgctcacctg cacgttcgaa gcgcgggaga attgcatcaa gccctcgaag gtgccgcagt
     8641 ccttggcccg gaatccagtc agcgcgaccg agtgggcctg gcccgtggtg tcgtgcccgt
     8701 tggtctgggc gacgaacagc aggatcgcac aggatttcgc ggtgatgtcc ttgaatacaa
     8761 gcggcccgct cggcttcgtg gacgagaagt cgtggtttgt cgaaagcgcc atgaagcacg
     8821 tgtcaagcgt cgtgccgctc acctccacat tgtggaaggt cccctcgaac tggaatccct
     8881 tgcctccgaa cgccccgccg gcgttgccgc gcgcgcaatt gcggatcgtg ccgccgttga
     8941 ccttgacatt cgaacaggtt ccaccgctca agccgttgtg gccggacgcc ccggcgaaga
     9001 gcccgctggc gttgatccgc ggattgttga cgactatgtt gtcgccgttc atgtcgatca
     9061 cggaaccgag cttcgtgtag tcgccgagca ccacttcggc ctcaaaaacg actgtggtgt
     9121 tgtccctgat tcgaagatag tcgtctatca gaagcgtggg atgagaaaaa ggggcggagc
     9181 caccatcgat gaccaccgtc ccgccaccgc ggttataggc ctcatccagc gccgcttgga
     9241 tcgcgagtcg ccacgacgag aacgaggtcg cgacgtggct gttcggatcg cctggagact
     9301 tcgcggtggc agtgaaaacc tggttcgcgt aatccgatac gctgatgcgc tcgtggccct
     9361 tgtcctgccc cgtgcgtcca gccgccgctg atgccggcag catgaacccg acgagacttg
     9421 cgccggcgca agctgcgtac gccgcacaga ccgcggcgcg gcgccgactg atcgactcgc
     9481 gtggattgat catgtcgtga tctcccgccg gcccggcgtc gccgcgaggt cgatattgca
     9541 agcgctcgag cttgcgagag ttatggcggc gtcgttgatc cggatattgc cgagttgcat
     9601 gtgagaatct cctcttgatg ctctgcgccg catcagccaa cttcccgagg gacgcacagt
     9661 gtgacatgac atgagcgcag ggccttgtgc gacagcgcga cccgcgcggg cgacggcacc
     9721 tgcgagccgc gctgcctgat caggcgttcg atcgcggacc ttgccgccct ggatcggcag
     9781 gatcccgccg tgcgcgcctc cccggcgagg acgcacgcct cccgccgcga cgcccgcgtg
     9841 cgggccgtct tcgcgatggc gctggccctg ggctcggcct tccgggcctg cggctcgacg
     9901 gccgccgcga ccggtccggc gtggatcgcg cggccgtgca gcgcgccgtc gccgaccgcg
     9961 cctatgcctt cttcgagtca gacccgtgct tcaccttggt gcacattccc gccgcactcg
    10021 acatcgacgc gaaaggtccg tcgcccatac tgcgactgtc ccgttccgcg accccgcgtg
    10081 gtcgacgtgt tgcttgctgc tcgcgaaccg cttacgcctc cgtcccggcc gccgcttgaa
    10141 ccgcctgcgc cgcgcggccc tgctgctggc caccggccaa cgcttcctgc tcgtcgatcg
    10201 gccgcgccag ctgccgcgcc gcgtcctttg gtactacgac tgggagaccc ttggcgacgc
    10261 cgtgatggac ctgtcccaga gagtcctcat cgatgcctcc atcgagctgg atctgtgcat
    10321 gcccaagggg ccgatcgagc tgttcgaaca cgacgcacgg tttcgtcgcg tgcatcggca
    10381 cctctccgac tgcaaaggcc cgtacgacct gatcgtcacc cagtgggtca cgacggagct
    10441 cattcgacgc aaggcgcggc atttccccct ggtgccatgg ctctccgtga tgtgccatga
    10501 gcgcggcgag ctgttttgcc gggcgacctt gagcttcgcg cggttgaggc cgctgttcgg
    10561 gccggactgc tcgtccacgg ccgttccgcc gtccttggat ctcgcgagct gcgccgcgtg
    10621 cggcttcgag cccgtggaca tcgtcgtcgc ggtgggcggc cgcgatccac gacgaaccta
    10681 ctcgcgctgg cccgaggtcg tggagacgct ggtgcgccat tggccgccgc agcgcggccg
    10741 tccgaccatc gccctcgtcg gcagcggcgc cgccgcgaag gtcgcggccg acgagattgg
    10801 ccgcttgggg ctggcggcgc gcatcgatgt ccacctcgac ctgccggggc cgctgcaggc
    10861 cgcccagctc atcgcggcgg gccaggtgtt tgtcggatgc gacggcgggc tcatgcacgt
    10921 cgcggccgcg ctcggcaagc cgggtgcggc gctgttctgc gagatccttc cgcgatggcg
    10981 gctgcatgcg acgtcgggca tcgtcggact cttcgatccg catggcatgg accgcatcga
    11041 agcggccgca gtcgcttccg cgacgttgag ggccgccggg ctggacgccc ctctttcttg
    11101 agcaggtcgg tcgagccgac ggcgaacagc gcgagcagcg ggccgctcac gccgcggcaa
    11161 ggcccatggg cgccctgtcg tcagccctcg cccggcggct cggtgccacc gagaaccgtg
    11221 gcggcgacca ggacgagcag gagcgtcgcg gcctcgacgt ggaggatgcg cgtcagcccg
    11281 cgccgccagc caccgccgtt ctcgaccccg gccagctcgg caaacagccg cggcagcacg
    11341 atgaagcggt tcgcgccccc gagtgccacg gccacggcga ccaaggccag cttgacgagg
    11401 agcgtgtggc cgtagtccga gtcgatgaac tgcctcgcgg acgccgacgg ctcgaaggcc
    11461 cgccacacct tgaagagccc gctcgcgccg atgcccacca gggagacggt cgccgtgctc
    11521 gacatgcgcg agacccagcg cgtcgcggct tgccgctcgg cgctcggggc gcaggccgcg
    11581 ggaagctcga gccgcgtgcc ggcggcgacg atcccgaccc agaggtacac gagcagcagg
    11641 tgcagccagc cgacggcgac gtcccgggtg aggtcgccgt cgctcccggc atgggacacc
    11701 gcggtgcgtg tcgcgatgaa gaccgccaag gcgccgagcg cgagggcccg gcgcgcggcc
    11761 gtgtcgcgag tgctgctgcg cgggatgagc gtcgcagccg ccagcagcca tgcaacgatg
    11821 ccgatcgagg tggcgcgccc gaagtggctg tgcgccagca acgtgccgac ggccgggccg
    11881 gcctgcagca gcggctgccc ggtcatgagg gccgcttcga accacggcgc cgcgagcgtg
    11941 cccgcgaggc cgagcgcgaa gcccaggcgc cgcatgccga gtgcttgttc cacgacccgg
    12001 tcgcgccagg ccgaaggctg gcgccacagc cagccctgcg acgcgacggc gccgaccacc
    12061 agcgcgaggc cgaggtggac ggtcgccgcg gccgccatcg tcaggatccg cagagcgccc
    12121 tcgcccatca cttcaccgcg aagcggatct cgcccttcgt gtggtggccg tcgtggccca
    12181 tcgtcgtcca ctgcacgcgg tactcgccgg cggcgagcct gggcagcggc tgcaccagcg
    12241 tcttgtcgtc gctcgggtcg gcaagcacct tgtcggtggc cacggcgacg tcacccgggc
    12301 cggtgatcct gacggtgctc atcgcgacct cgacgggctc gttgtagcgc aggcgcagct
    12361 cggcggacgc agggctgacc gccgcgccgc tggccggggt cgaggcctgg agcttggcgt
    12421 gcgcgagcgc gagggccgag gccagcgtca gggcgatgaa tgcaaggggc ttcttcatgg
    12481 cggatcctct tcggagcgat tggcagttca gtggccttca tggccggtgg gcttgtggac
    12541 gtgcaaggtc gcggcgtcgc tcgcgggctg gctcgcctgc ggcgccggcg ccgcgtcgtc
    12601 cgcggtgtat tcgctggcga cggtgccctg cggatgccgg aaccagccgg ggtcgcggta
    12661 gtcgcccggc tcgacgccgt cccgcacctt gaccacgctg aacatgccgc ccatctcgag
    12721 gccgccgtag ggcccctccc cgctcatcat cggcagcgtg ttgtcgggca atggcatccg
    12781 catctcggcc atgtccgaca tgccggacga gcccatggcc atgtagccgg gcacgagcct
    12841 ggcgaccttt tccgcggcgg cgcgctggtc gacgccgatc atgttggcgg ccgtgtgcga
    12901 catcgggccc atcgtgtggt gcgacttgtg gcagtggatc gcccagtcgc ccgcgaccgc
    12961 gtcgaactcg atcgcgcgca tctggccgac ggccacgtcg gtggtgacct ccggccagcg
    13021 cgcggacttc gggatccagc cgccgtccgt gccggtgacc tcgaagtgcg gcccgtgcat
    13081 gtggatcggg tggttggtca tcgtcaggtt gcccacgcgc acgcgcacgc ggtcgcccgt
    13141 gcgcgccacg agcgggtcga tgccggggaa cacgcggctg ttccaggccc acaggttgaa
    13201 gtccgtcatc tcgctggtgc gcggggtggc ggcccccggg tcgatggcgt agctcgccat
    13261 gacgaacgcg aaatctcggt cgacccggag ctggcgaacg tcgcggggat ggatgatgaa
    13321 caggcccatc atgcccatgg ccatctgcac catctcgtcg gcgtgcgggt gatacatgtg
    13381 cgtgccgggt cgctgcagca cgaactcgta gacgaaggtc ttgccgggcg ggatgtgcgg
    13441 ctgcgtgagg ccgccgacac cgtccatgcc gttgggcacg agcacgccgt gccagtgcac
    13501 cgtcgtgtgc tccggcagct tgttcgtcac gaagatgcgc acgcggtcgc cttcgaccgc
    13561 ctcgatggtc ggcccggggc tggaaccgtt gtagccccac aggttggcct tcatgccggg
    13621 cgcgagctcg cgcacgacgg gctcggcgac gagatggaac tccttgacgc cgtccttcat
    13681 gcgccacggc aggctccagc cgttgagcgt gacgaccggg tggaagggtc gacccgtggg
    13741 cggcgacggc ggcggttgca tggtcggcga cgtctggctg gccgcctcgg gcagcatcgc
    13801 cgcgccggcg cggctgacgg ccgccgcgcc cagcatcgtc gcgccggcgg ccttgaaaaa
    13861 gcttcttcgg tgggtcatgg cagtcttcag ggatgggttg gcgaggcgcc ggcgagcgct
    13921 tcctgcaggc gggcatcggc gagccagaag tcgcgggtgg cctcgacgga cgccgtcacg
    13981 ctcgcgacct gctcgcgcgc atcggcgagc aggtcgaaga cgtcggcgag catcccgttg
    14041 tagcgaagca aggtctcctc ggagatgcgc tggtgcagcg gcaccacctc gtcgcggtag
    14101 tgccgggcca ggtcgaaggc gccccggtag ccggcgtagg cctcgcgcac ttcggagcgc
    14161 gcgtcgacag cggtggccgc ggcgcggttg acggcctgca ggtacacggc ctgcgcgcgg
    14221 gcgcggtcgg catcgccgaa gtcgaagatc ggcagctcga attgcacctg gccgccgcgc
    14281 tccggcgccg cgccgcgctg gcgcttgtcc tgccagccgg cgtcgagcgc gttgacgaac
    14341 ccggtgaccc gggtcaggcc cagctgccgc gccgtcgcct cggtttcccg tcgggccatc
    14401 aggacgtcca ggcgcttgtc gagcgccgcc tgctcggcgt cctcgggcgc ggccacgtcc
    14461 tggggcaggt cgggcagccg ttcaggcaat cggagcagcg cctgctggtc gcccaggccg
    14521 agcgcgcgca cgagccgttc gcgcgccgac agcgcctgct ggtggctgtg cgcggcctgc
    14581 gccatcgcgt ccgcgtggaa cgcctgctcc cgcatctgct ggagcttgct gacgttgccc
    14641 gcgcgcagca ggcccttggc gagctcgtcg gcggcgtccg cggcttccgt gacctggcgg
    14701 gcgtaggcgt cgagctgctg cgccgcgacc gcatcgaagt aggcctgccg cgcctgctgg
    14761 gcgactgcca cggcgtcggc ggcggcctgc agctgcgcct ggtcgaagcg atcccgggcg
    14821 atgcgagagc ggcccggcag cgtgagcagg cccagcacgt cgaacatcac cgagcgatcg
    14881 atctccagcg cgtcgccgcc cgcgagtcgg ccgagcgaga tcgccggatt gcgaagtcgt
    14941 ccggcctgca ccaggtcggc ctcggagatg ccgacgttgc cgagctgctg ctgcagggcg
    15001 gggttgttca gcagcgccac ctcgacggcg ccgtcggcgg acagcggcgc ccgcagcagc
    15061 tcggccacgc gggatcgcgc ggcctgggtg tcctgccgat cacgctggag ggcgaccgga
    15121 tgcccggtgc gctcctgcgt gagccgggag acgcgcgaca tcccgccgtc ggcggaaaag
    15181 ctcgcgcaac ctgcgagcgc aagggccgcg gtcgcggcca gtgtcaggcg cgggatcgat
    15241 gggtggcgcg cacgggcagg ccgccgtcgg ggcgcttgcc cctgggagtc gtggttcatg
    15301 ggtcgaagtc atctggcgcg tcagccggag ccggctgcgc aacagcccgt gcgcggggca
    15361 cgagcacatc ccggcctcgg gcggaggcct cgcggggtca gatgcttcgg ggtgggcgct
    15421 ccaggccttc cgacaggaac gtcggcgccg aggcctggag caaggggaac gtcgcgacct
    15481 gatcgcccag ggcggccacc gtcggcgaga acgtcatgag catgggcgtc gccgagcagc
    15541 atgacgcgca caggttgcac ttgtcgaccc cggcatggtc gtggtgcgcc gcgccgtgac
    15601 cggtctcgct cgcggcgtcc ggttcgacgg ccgcgtgcat ggggtggtgc gtgtgcatcc
    15661 gctgcgcggg cgcctgatgc gcgaggccct cgcagggcga cgcggccgcg atcgcgcccc
    15721 gcgcggggag caggatcgcc aacagcacga gcagccagat gcgaacgagt ttcacgaacc
    15781 gaaggttagc acggggtcga atggctgcgg cgtcaccctt gtccgaccgg ctcgcgccgc
    15841 tccatcaggc tctcgcgcac tgccacgatg tgcccgcgca gggcgtagag cacgtcgccg
    15901 taggccggcg gcacgacgag ccgggcgacc gtcgactcga cgtgcgcgag ccggtgcagc
    15961 agctcgtcgc agcggcgcgc gtcgagcggc tgctcgtagg agtcgcggtc gatgcgctgc
    16021 agcaccgcat accagcggta gatgcgcgac tcgatgcgcc agcgaaagac cgccggcgcg
    16081 atcttgatgc cggggatcag cacgatggtc atcggcacga ccaccgccag cacacgctcg
    16141 atcaggctgg cgatccagaa cgggaacgtg cggtagaaga agccgcggcc cgacgcgtag
    16201 tagcgcgagg cgtcggcgct gatgggcagc tcgtgctgga tcggcgccgg gaattcgccg
    16261 ctcttcttgt acaggccggc gcggccgtgg acctcgcgcg ccgcctccag cagcaggtcc
    16321 gacagcgccg gatgcagcga atcgcgcgcg accagctcca ccgtcggcgc gacgagcttg
    16381 acgtcctcgg ccgggacgtc ctcgccgaag tcgatcgccc cgcgcggcag cacgagccgg
    16441 ttcaggtagg tgatgcggtg cgtgtagccg tcggcctgcg cgaagctgta gaggtggatg
    16501 tcgggatcgc gcagcagctg gcgcatcagg cccgtgggcg tggagtcgct catcgcgaac
    16561 agcgcgtcga tgcggccgcc cagcagctcc ttgaccgagg cctcgggcgc ggcgtggccg
    16621 atgcgcgtgc cgtcgcccgg cttgatgccg ttcgcctcga acagccgctg cgacagcgcc
    16681 gacgcgccgc tgccgtcctg gccgatgtcg atgcgttctc ctttgaactc cgagatcagc
    16741 gtcttcggac ggccgcggta gaagatcatc agcggctggt acgacacgct gccaagcgac
    16801 atcagccgcc ggccggcgtc gccttcgggc gcctcgccgc cgacgacgaa gccgacgtcg
    16861 acgtcctgct tcggatcggc cagccgcgcc aggttgtccc tggagccgcg cgaggtcacg
    16921 atccgcagcg tcacgccctc gcgcgcgagg atcttgcggt attgctccgc cacgagccgg
    16981 aacgagctgc cctcggggcc gctggtcatc gtcagcgtgg tcggcggcgc cgaattgagg
    17041 aagatgaagg cgagcgcgcc gacggccagg ctcacgagca cgatcaggcc gatgctgatc
    17101 ggcaggccgc gaccgaccag gccggtcagg cggacacggg tcagctcgag ttgcgcgcgc
    17161 agcgtgctgg aggattcggg cgtcggggac atgggcgggc gccgtgcgat gacgcggcat
    17221 cttaattggc cggtgtgtcc ggggccttgc gcgacccgca tgcgcggcgc acgcttgcgc
    17281 gacccgtgcg cgcggcggtc aatcccctgg agcgatccgg cagccgtccc ccgccgcggc
    17341 gacaatgcgc tcgcccggcg agcggccgga tcacgggaga cggcgggaat gggtgccttc
    17401 tggtcgaact tcaggcgcgg cgtcgcggag ctggtccgcg cggtctcggc ctgcgtgttc
    17461 ccggtcttca gcctgctcgc gtggttcggc atcctcctcg ccggccaggg cgaggacgtc
    17521 gtgcgccgca gcgtggagcg cgccgccctc gacgacagcg cgtcggagct gatcgccctc
    17581 gccatcgcgt cggtgctgct ggggctgtcc gtctggtatg cgatgcgcag cctgctgagc
    17641 gtccagcgcc ccgggctgcc gccgcccaac cccgggccgc acgggccgac ggcgtggctg
    17701 ccgcggatcc tcggcgcgct ggccgtggcg tggccgggat gggtcgcgca cgacgcggtc
    17761 g
//
LOCUS       DASVLN010000411         5391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_74784, whole genome shotgun sequence.
ACCESSION   DASVLN010000411 DASVLN010000000
VERSION     DASVLN010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74784"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..579)
                     /locus_tag="VF453_10225"
     CDS             complement(76..579)
                     /locus_tag="VF453_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688075.1"
                     /translation="MNNKLLKLAAVALVAAAAGAAQAQISVGASISINEPGVYGRIDL
                     GPSAPVYAPPAVVYPQPVVIEPAPIAVHRAPIYLYVPEAYTRDWAHHCRAYGACGQPV
                     YFVREDWVRERYVHFHPNDHRYDHWHEREWHEHDHDHDHDRGWHGHGHGHDDDDDHDH
                     GRGHGHH"
     gene            842..1006
                     /locus_tag="VF453_10230"
     CDS             842..1006
                     /locus_tag="VF453_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688076.1"
                     /translation="MGNKIVTEADKKATPRPALPKGVTATTGKGQKVSLERGSHTRSK
                     KNPGKRARQG"
     gene            1206..2894
                     /locus_tag="VF453_10235"
     CDS             1206..2894
                     /locus_tag="VF453_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688077.1"
                     /translation="MIRITELRLPLNHAEDALRPAVLARLGVADAQLAGFTIFKRGYD
                     ARKKTAIVLVYTIDAELVDGARAEAAVLARLAGDAHVRPAPDTRYRFLGHAPADFFAA
                     ERPRPVVVGFGPCGLFAALILAQMGLRPIVLERGRQVRERTQDTWGLWRRGELDPDSN
                     VQFGEGGAGTFSDGKLYSQISDPRHLTRKVLAEFVKAGAPAEIELVSKPHIGTFRLVG
                     MIEKMRADIVALGGEVRFGQRVADLLVEGPPDARRVRGLRLASGEELRADHVVLALGH
                     SARDTFAMLHARGVRMEAKPFSIGYRIEHPQGLIDRARFGPNAGHELLGAADYKLVHH
                     AGNKRSVYSFCMCPGGTVVAATSEPERVVTNGMSQYSRNERNANAGIVVGITPEDYRQ
                     SGAPGTPVDPLDGIAFQRFWESRAYELGGSGYRAPGALVGDFVKRQRSSAFGSVMPSY
                     KPGVTLTDLAEPGRGSLPDYALDAIREALPAFDRQVKGFFMADAVLTGVETRTSSPLR
                     IPRGRDYQSLNVRGLYPAGEGAGYAGGIMSAGVDGIEVAEALAADLLGLQPPTR"
     gene            3019..3678
                     /locus_tag="VF453_10240"
     CDS             3019..3678
                     /locus_tag="VF453_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7688078.1"
                     /translation="MYTLYGSRGSGSAAAECGLEMAGVDYRIVAAATWEPGPALDELT
                     RVNPLHQIPTLVLPSGQVMTESAAILMHLGLAFPQSGLLSEEPAERDRALRGLVYIPA
                     NCYSCITVIDYPERFTTATDDAARAAVREGTRARLHHHWDVFADQFPVDGDRFLGGTQ
                     PGALDILAAVVSKWSGTRQHLKTSRPAFSALLERIESHPSVALVFGRHWPTAAELPTG
                     A"
     gene            complement(3724..4068)
                     /locus_tag="VF453_10245"
     CDS             complement(3724..4068)
                     /locus_tag="VF453_10245"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX7688079.1"
                     /translation="MSTIDVQVPGIRKRLRVRTADTLGRRALAMLKPGEPRDAYGLWV
                     KPCRTVHTFGLSDAVDVIFLRADGVVTKVVSRMKPWRMTRCSEARTVLELRAGLANRL
                     NIAPGVALDLLA"
     gene            complement(4368..4970)
                     /locus_tag="VF453_10250"
     CDS             complement(4368..4970)
                     /locus_tag="VF453_10250"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX7688080.1"
                     /translation="MSTPPWNFSLEPARDKPTLRRQLKAERMAMPDRHQRSVHLQEVL
                     RVWLVTRPETSIGAYWPIKGEFDALPALFRWTEGEAYGRLIGLPVIDRATKQLDFHVW
                     YPGCPMEDDAYGIPKPKGTDKFEPELLLVPCVGYGDNGVRLGYGGGFYDRTLAALTPR
                     PFTVGIGYAHGYVPWLEPEPHDVPLDAMITEDGVFWEREG"
     gene            5058..>5391
                     /locus_tag="VF453_10255"
     CDS             5058..>5391
                     /locus_tag="VF453_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX7688081.1"
                     /translation="MQDSGLVDHCLELLAPLGRTRAGRMFSGYGLYVDDLCIAFILRD
                     TLYLKVDDAHRADFEAAGCQPFTYETRKGERQSISYYSAPGEAMESPAEMRPWARRAL
                     EAAVAARAK"
BASE COUNT          737 a         1953 c         1931 g          770 t
ORIGIN      
        1 caggtagcgc ggcgcgcggg gcgaccgcgc agccgccgcg ccggcaacgg cgccgccggc
       61 cggcgtcatg gccgatcagt ggtggccgtg cccgcggccg tgatcgtggt cgtcatcgtc
      121 gtcgtgaccg tggccatggc cgtgccaacc gcgatcgtgg tcatggtcat ggtcgtgctc
      181 gtgccactcg cgctcgtgcc agtggtcgta gcggtggtcg ttcgggtgga agtgcacgta
      241 acgttcgcgc acccagtcct cgcgcacgaa gtacaccggc tggccgcagg cgccgtacgc
      301 gcggcagtgg tgggcccagt cgcgggtgta cgcctcgggc acgtacaggt agatcggggc
      361 gcggtgcacg gcgatcggcg cgggctcgat caccaccggc tgcgggtaga ccacggccgg
      421 cggcgcgtac accggggccg acgggccgag gtcgatgcgg ccatagacgc ccggctcgtt
      481 gatcgagatg ctggcgccga cgctgatctg ggcctgcgcg gcccccgcgg cggcggcgac
      541 gagggccacg gcggcgagct tgaggagctt gttgttcatg cgttccatcc ttcgagcgcg
      601 aagctcgtcg aagaggcctc gtcccgcggc cgacggcggc gccgtcgcgg gccgcggggc
      661 ccctgccctc gtccaacggc agcgtcgggg cttgcgttga cccttcattc gtaacgtttg
      721 tttgcgatcc gcgcgtcgcg tttcacgccc gcgatccgct cgcgaacgcc gattttcccc
      781 cgcccgtggt gtagaatgct gggttcgacc ctttttcagc ttttcaagac cggatccaat
      841 catgggcaac aagatcgtta ccgaagccga caagaaggcc acgccgcgtc ccgcgctgcc
      901 gaagggcgtc accgccacga cgggcaaggg ccagaaggtc agcctcgagc gcggctcgca
      961 cacgcgcagc aagaagaacc cgggcaagcg cgcccgccag ggctgaagcg ccccggggcg
     1021 accggcctgc gccgtcgcct cgcccgccgc cggggccgcg cggccccgtc gtccgctcag
     1081 gagccctcgg gctcctttgt tcgttgtccc gccgattcgt cgccgcggct gccgcgccgt
     1141 ccccccgccc tgcgcgatga gatgatcgct gtcgccgccg gccgtcacgg gccggcgtcc
     1201 ccgccatgat ccgcatcacc gaactgcgcc tgccgctgaa ccacgccgag gatgcgctgc
     1261 ggcccgcggt gctcgcgcgc ctgggcgtcg cggacgcgca gctcgccggc ttcaccatct
     1321 tcaagcgcgg ctacgacgcg cgcaagaaga cggcgatcgt gctcgtgtac acgatcgacg
     1381 ccgagctcgt ggacggcgca cgcgccgagg ccgccgtgct cgcgcgcctg gcgggcgacg
     1441 cccacgtccg ccccgcgccc gacacgcgct accgcttcct cggccacgcg ccggcggact
     1501 tcttcgcggc cgagcgcccc cgccccgtcg tcgtcggctt cgggccctgc ggcctgttcg
     1561 cggcgctgat cctcgcgcag atgggcctga ggccgatcgt gctggagcgc ggccggcagg
     1621 tgcgcgagcg cacgcaggac acctggggcc tgtggcgccg cggcgagctc gatcccgact
     1681 cgaacgtgca gttcggcgaa ggcggcgccg gcaccttctc cgacggcaag ctctacagcc
     1741 agatcagcga cccgcgccac ctgacgcgca aggtgctcgc cgagttcgtc aaggccggcg
     1801 cgccggcgga gatcgagctc gtctcgaagc cgcacatcgg cacgttccgg ctggtgggga
     1861 tgatcgagaa gatgcgtgcc gacatcgtcg cgctcggcgg cgaggtgcgc ttcgggcagc
     1921 gcgtcgccga cctgctggtc gaggggccgc cggatgcgcg ccgcgtgcgc gggctgcggc
     1981 tcgcgtcggg cgaggagttg cgcgccgacc acgtcgtgct ggcgctcggc cacagcgcgc
     2041 gagacacctt cgcgatgctg cacgcgcgcg gcgtgcgcat ggaggccaag ccgttctcga
     2101 tcggctaccg catcgagcat ccgcagggcc tgatcgaccg cgcgcgcttc ggcccgaacg
     2161 ccggccacga gctcctgggc gcggccgact acaagctggt gcaccacgcg ggcaacaagc
     2221 gctccgtgta cagcttctgc atgtgcccgg gcggcaccgt ggtcgcggcg acctccgagc
     2281 cggagcgcgt cgtcaccaac ggcatgagcc agtactcgcg caacgagcgc aacgccaacg
     2341 ccggcatcgt cgtgggcatc acgccggagg actaccgcca gtcgggcgcg cccggcacgc
     2401 cggtggatcc gctcgacggc atcgccttcc agcgcttctg ggagtcgcgc gcctacgagc
     2461 tcggcggcag cggctatcgc gcgccggggg cgctcgtcgg cgacttcgtc aagcgccagc
     2521 gctcgtcggc attcggcagc gtgatgccgt cgtacaagcc aggggtgacg ctgaccgacc
     2581 tcgccgagcc cggccgcggc agcctgcccg actacgcgct ggacgcgatc cgcgaggcgc
     2641 tgccggcctt cgaccgccag gtcaagggct tcttcatggc cgacgccgtg ctcacgggcg
     2701 tcgagacgcg cacgtcctcg ccgctgcgca tcccgcgcgg ccgggactac cagagcctga
     2761 acgtgcgcgg cctgtacccg gccggcgagg gcgcgggcta cgccggcggg atcatgtcgg
     2821 ccggcgtgga cggcatcgag gtcgcggagg cgctggccgc cgacctgctg ggcctgcaac
     2881 cgccgacgcg ctgatccggc cgtccggcgc cgtcgatccg ccgttcgaac ccacaccggg
     2941 cgcacgggcc gcgcggcgcg cattagcatc cacccttcca tggcatcgaa cgcggtcgcg
     3001 cgaccgggaa gggcaggcat gtacacgctg tacggatcca ggggctccgg ctcggccgcc
     3061 gccgaatgcg gcctcgagat ggcgggcgtc gactaccgca tcgtcgccgc cgcgacctgg
     3121 gagccgggcc ccgcgctgga cgaactcacg cgcgtcaacc cgttgcacca gatcccgacg
     3181 ctcgtgctgc cgtccggcca ggtcatgacc gaaagcgccg ccatcctgat gcacctgggc
     3241 ctcgcgttcc cgcagtccgg cttgctgagc gaggagccgg ccgaacgcga ccgcgcgctg
     3301 cgcggcctgg tctacatccc ggccaactgc tactcctgca tcacggtgat cgactatccc
     3361 gagcgcttca ccaccgcgac cgacgacgcc gcgcgcgccg ccgtgcgcga gggcacgagg
     3421 gcgcggctgc atcaccactg ggacgtcttc gccgaccagt ttcccgtcga cggcgaccgt
     3481 ttcctcggcg gcacgcagcc cggcgcgctg gacatcctgg ccgcggtcgt ctcgaaatgg
     3541 tcgggcacgc gccagcacct gaagacgtcg cggccggcgt tctcggcgct gctcgagcgc
     3601 atcgaatcgc atccgtcggt ggcgctggtg ttcgggcggc actggccgac cgcggcggag
     3661 ttgccgacgg gcgcttgagc cggcctcgcg cgcgtcacgc gcgcgaggcc tggggcccgg
     3721 gggtcaggcc agcaggtcga gcgccacgcc cggcgcgatg ttcagccggt tggccaggcc
     3781 ggcgcgcagc tcgaggacgg tgcgcgcctc ggagcagcgc gtcatgcgcc agggcttcat
     3841 gcgcgagacg accttggtga ccacgccgtc ggcgcgcagg aagatcacgt cgaccgcgtc
     3901 gctcagcccg aaggtgtgga ccgtgcgaca gggcttgacc cacagcccgt acgcgtcgcg
     3961 cggctcgccc ggcttgagca tcgcgagggc gcggcggccc agggtgtcgg cggtgcgcac
     4021 gcgcaggcgc ttgcggatgc cgggaacttg cacgtcgatc gtggacatgg agggggcttc
     4081 ctggaaactg cgtctcgatc cgcggcgggt ggcgggatcg tcgttgtcga ccggcccctc
     4141 tgcgtgagcc gactcccgcg atggcgggag cccgcagctt accgtcgagc cgtgtgaaag
     4201 atcacaccca cctcgggtaa acgatgagct tcgaacagat cgttacgtgt ctgcgcgaca
     4261 gctttggatc caggtgtttc gaagccttga caggtcgccc cgccgtgtcg cccgtggcac
     4321 gctcgcgccg gcgcgatggg cgcgcaagcg ccatcgccgg tgacggctca gccctcgcgc
     4381 tcccagaaga cgccgtcctc cgtgatcatc gcgtcgagcg gcacgtcgtg cggctccggc
     4441 tccagccagg gcacgtaccc gtgggcgtat ccgatgccga ccgtgaacgg gcgcggcgtc
     4501 agcgcggcga gcgtgcgatc gtagaagccg ccgccgtaac ccaggcgcac gccgttgtcg
     4561 ccgtagccga cgcagggcac cagcagcagt tcgggctcga acttgtcggt gcccttgggc
     4621 ttggggatgc cgtaggcgtc gtcctccatc gggcagccgg ggtaccagac gtggaagtcc
     4681 agctgcttgg tcgcgcggtc gatgaccggc aggccgatca gccggccgta ggcctcgccc
     4741 tccgtccagc ggaacagcgc cggcagggcg tcgaactcgc ccttgatggg ccagtaggcg
     4801 ccgatggagg tctccggccg cgtcaccagc cagacgcgca gcacctcctg caggtgcacc
     4861 gagcgctggt ggcgatcggg catggccatg cgttcggcct tcagctgccg gcgcagcgtg
     4921 ggcttgtcgc gggcgggctc gagcgagaag ttccagggcg gcgtcgacat gacgcaggat
     4981 tatggactcg cgcggcccgc catcgcagga tggccttcgc ctgcgcgcgg cgggactccg
     5041 aactaaactc gacggccatg caagactccg gcctcgtcga ccactgcctc gaactgctgg
     5101 cgccgctggg tcgcacgcgc gcgggacgca tgttcagcgg ctacggcctg tacgtcgacg
     5161 acctgtgcat cgcgttcatc ctgcgcgaca cgctgtacct gaaggtcgac gacgcccacc
     5221 gcgccgactt cgaggccgcc ggctgccagc ccttcaccta cgagacgagg aagggcgagc
     5281 ggcagtcgat cagctactac agcgcgccgg gcgaggcgat ggagtcgccc gccgagatgc
     5341 gcccgtgggc gcgccgtgcg ctcgaggcgg cggtcgccgc gcgcgcgaag g
//
LOCUS       DASVLN010000412         2215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_396766, whole genome shotgun sequence.
ACCESSION   DASVLN010000412 DASVLN010000000
VERSION     DASVLN010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396766"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..724)
                     /locus_tag="VF453_10260"
     CDS             complement(23..724)
                     /locus_tag="VF453_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7688082.1"
                     /translation="MKVLLVDDHPLILSALQAMIQELDPDVRVTAVGSASAARSALAA
                     DADFDLVLLDLQLGDASGFDVLVEMRKAHPALPVVVISASDRASDVIQAIDLGAMGFV
                     PKRASNDTLVEALRLVMAGGIYVPPMHLGANVGSGPGQRLAAIPAADAPGYQVTPSFE
                     ELGLTPRQADVLTQLLQGKPNKEIARRLGLSVETVKDHVQAVLRALGVSSRTQAVLAV
                     SQMTQQRLERRALAR"
     gene            complement(942..1589)
                     /locus_tag="VF453_10265"
     CDS             complement(942..1589)
                     /locus_tag="VF453_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX7688083.1"
                     /translation="MPVVVVANPKGGAGKSTLATQIAGFLASTGRTVTLGDLDRQQSS
                     RQWLAVRPPGLPRISPWEISRDAIAKPAKGTTDAVIDTPAGLHGARLDAALKVADRVV
                     VPLQPSLFDMQATHAFLHALRGHKRAERLRVGLVGMRVKEHTIAHERFQEFVATLGFP
                     LLTTLRDTQNYVHLAAHGLTLFDVAPGRVERDLAQFAPIARWLTGADEPAAEPRE"
     gene            1667..>2215
                     /locus_tag="VF453_10270"
     CDS             1667..>2215
                     /locus_tag="VF453_10270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7688084.1"
                     /translation="MADVLVLVAHPDIARSRINRALAAEARRQAPGDVEVRDLYALYP
                     DFEIDVASEQAALAAAGTVVWVHPVQWYSMPALMKLWVDEVLAFRWAYGPGGRALAGK
                     CLWLVASTGGDVASYRPDGNNRYPFEHFLPAYRQTAELAGMRFLDPSIFHGAHRADDA
                     AVAAHVRAFGERLRALAHENRAA"
BASE COUNT          288 a          808 c          818 g          301 t
ORIGIN      
        1 cgcggccggc agcggcgcgc ggctagcggg ccagcgcgcg gcgctcgagc cgctgctgcg
       61 tcatctggct gaccgccagc acggcctggg tgcgcgagct cacgccgagc gcgcgcaaca
      121 ccgcctgcac gtggtccttg accgtctcga cggacaggcc gaggcggcgc gcgatctcct
      181 tgttcggctt gccctgcagc agctgggtca gcacgtcggc ctggcgcggg gtcaggccga
      241 gctcctcgaa cgagggcgtg acctggtagc cgggcgcgtc cgccgccggg atggccgcga
      301 gccgctggcc cgggccgctg ccgacgttgg cgccgaggtg catcggcgga acatagatgc
      361 cgccggccat cacgaggcgc agcgcctcga ccagcgtgtc gttggaggcc cgcttgggca
      421 cgaagcccat cgcgccgagg tcgatcgcct ggatcacgtc gctcgcgcgg tcggaggccg
      481 agatcacgac gaccggcagc gcggggtgcg ccttgcgcat ctccaccagc acgtcgaagc
      541 cgctcgcgtc gccgagctgc aggtccagca gcacgaggtc gaagtcggca tccgccgcca
      601 gggccgagcg ggccgcggac gcgctgccga ccgccgtcac gcgcacgtcg ggatccagct
      661 cctggatcat cgcctgcagc gccgacagga tcagcgggtg gtcatcgacc agcaagacct
      721 tcatgaaatt tctgctccgg attaccgcca cgccgggggg ctggcgattc gcgttcgggc
      781 gatcgtatcg gtttgggctg cacttgaaag accccctaaa gggggatacg gccgcatcgg
      841 acgcgacggc ccgtgaccca ttgcacggcc gccggcggcc ggcaggacgt cccgcgctgc
      901 gcgcaccggt tagcgccggc agcgcgcgcg tgccgcgggc gtcactcgcg cggctcggcg
      961 gcgggctcgt cggcgcccgt gagccagcgc gcgatcggcg cgaactgggc gaggtcgcgc
     1021 tcgacccggc cgggcgcgac gtcgaacagg gtcagcccgt gcgcggccag gtgcacgtag
     1081 ttctgggtgt cgcgcagcgt cgtcagcagc gggaagccga gggtggcgac gaactcctgg
     1141 aagcgctcgt gcgcgatcgt gtgctccttg acgcgcatgc ccacgagccc gacgcgcagc
     1201 cgctcggcgc gcttgtgccc gcgcagcgcg tgcaggaagg cgtgcgtggc ctgcatgtcg
     1261 aacaggctgg gctgcagcgg cacgacgacg cggtcggcca ccttcagggc tgcgtccagg
     1321 cgcgcgccgt gcaagccggc cggggtgtcg atgaccgcgt cggtcgtgcc cttggcgggc
     1381 ttggcgatcg cgtcgcgcga gatctcccac ggcgagatcc gcggcagccc gggcggccgc
     1441 acggccagcc actggcgcga ggactgctgg cgatcgaggt cgcccagcgt caccgtgcgc
     1501 ccggtcgagg cgaggaagcc cgcgatctgc gtggcgagcg tgctcttgcc cgccccgccc
     1561 ttggggttcg cgacgacgac gaccggcatg gatggcttct cctcgggttc ccgactgctc
     1621 ggcggcagcc gaacgcgatg gtaccgaacc cgcgacaatg cggcgcatgg cagacgtcct
     1681 ggtgctggtc gcccatcccg acatcgcgcg ctcgcgcatc aaccgcgccc tcgccgccga
     1741 ggcccgccgc caggcgcccg gcgacgtcga ggtgcgcgac ctctacgcgc tctatcccga
     1801 cttcgagatc gacgtcgcgt ccgagcaggc ggcgctcgcg gcggccggca cggtcgtgtg
     1861 ggtgcatccg gtgcagtggt actcgatgcc ggcgctgatg aagctgtggg tcgacgaggt
     1921 gctcgccttc cgctgggcct acgggccggg cgggcgggcg ctggccggca agtgcctgtg
     1981 gctggtggcc tccaccgggg gcgacgtcgc gtcctaccgc cccgacggca acaaccgcta
     2041 cccgttcgag cacttcctgc ccgcctaccg ccagaccgcc gagctcgcgg gcatgcgatt
     2101 cctcgacccc agcatcttcc acggcgccca ccgcgccgac gacgcggccg tcgccgcgca
     2161 cgtgcgcgcg ttcggcgagc gcctgcgcgc gctcgcgcac gagaatcgcg ccgcg
//
LOCUS       DASVLN010000413         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_342856, whole genome shotgun sequence.
ACCESSION   DASVLN010000413 DASVLN010000000
VERSION     DASVLN010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342856"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF453_10275"
     CDS             complement(<1..252)
                     /locus_tag="VF453_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688085.1"
                     /translation="MTRHPRPGLLAVAAALAGCAQMPTGPTVAVGPGPYKPFEVFVQD
                     DQLCRGWAAHSIGLPGNDEAARAFIGSTAVGAAVGALAGA"
     gene            complement(260..709)
                     /locus_tag="VF453_10280"
     CDS             complement(260..709)
                     /locus_tag="VF453_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006027209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688086.1"
                     /translation="MAHPPSRSRSARRAALAIATAATAAIAAAQPHGGHRPGGLGWHG
                     DIRHFGDHDWHVWRGGRWVHGPHGGHPGWWWVVGPTWYFYPSPVYPYPDPYEPPPPWT
                     VPMPAPPARFWYFCEESRSYYPYVATCPGGWKQVPAVQAPDASAPVQ"
     gene            1104..1757
                     /locus_tag="VF453_10285"
     CDS             1104..1757
                     /locus_tag="VF453_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7688087.1"
                     /translation="MKTDKQLKDDVEAELEWEPSIDAAHVGVAAKDGVVTLTGHLNTF
                     AEKAAIERIAARVAGVRAVAVELDVKVAAEHVRSDTEIAEAARNAVAWNVVVPRDKVQ
                     VKVEKGWVTLSGELDWEYQREAALSSVRALKGVRGVTSSLALKQRVLSSDIGARIGTA
                     LARHAYREAGGIHVGVSGSTVTLRGKVDSWAERAAAEGAAWSAPGVTAVVSDLTIGR"
     gene            complement(1820..3355)
                     /locus_tag="VF453_10290"
     CDS             complement(1820..3355)
                     /locus_tag="VF453_10290"
                     /inference="COORDINATES: protein motif:HMM:NF027442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HEX7688088.1"
                     /translation="MARPPTPWRLAAAVDDGSAASPARRGDKRRAVTDRVLRLGQFAT
                     ACAMLAAACSGGTSSSQPVQAPAPPASPSPAPGAPPGTTTATFAASGANYPNPDRGFY
                     GSAGDDFVAGFDPASVQSVYASGRRLLIASVSLAAFRTGDLSAAFLSTLGDRFAAVRS
                     AGMKTTLLFAYDFSGSGLDASSTRIARHLEQLRPVLAANADVIPYMRAGFIGAWGEWH
                     SSQSGNSCDEGSAPTSCATAAANRAIVRDALLRNVPPTTQVDFRYPSDLQAWYPDGDA
                     PAQVGMDDDCFLAGPSDTGTYTQPGQRAYAQLLTREGSFGGETCENAETPIRGTCADI
                     LSEGALYHLAWLNGDYAPSVVDGWKNAGCYDQVAASMGYRIQLDRVSNAPAVARGGTL
                     AVHVDLRNVGWARMFVARALVVTIRRRDTGETLAASAGNLATLAPQATASTRIVLQLP
                     IPAGAATGLYDVFVSAPDAFAATQDDVRFSVRFANADRPAAGQSWDASTARLSTGTTV
                     TVE"
     gene            3490..>3737
                     /locus_tag="VF453_10295"
     CDS             3490..>3737
                     /locus_tag="VF453_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688089.1"
                     /translation="MTYPTVLLHLDDDARDEARNEQAVRLALHLGCHLNGLACPRPVP
                     AATGFASSLGGLDDAFALELEHAREQARVRERRFLGLC"
BASE COUNT          492 a         1322 c         1453 g          470 t
ORIGIN      
        1 cgcgccggcg agcgcgccga cggccgcacc cacggccgtc gagccgatga acgcccgcgc
       61 cgcctcgtcg ttgccgggca ggccgatcga gtgtgcggcc cagccgcggc acagctggtc
      121 gtcctgcacg aagacctcga acggcttgta ggggcccggt ccgacggcga ccgtcgggcc
      181 ggtgggcatc tgggcgcagc cggccagtgc ggcggcgacg gcgagcaggc ctggcctcgg
      241 atggcgcgtc atcgccgcct cactgcaccg gggccgacgc gtcgggcgcc tgcaccgcgg
      301 gcacctgctt ccagccgccc gggcaggtcg cgacgtaggg atagtagctc cgggactcct
      361 cgcagaagta ccagaagcgc gccggcggcg ccggcatcgg caccgtccag ggcggcggcg
      421 gctcgtacgg gtcgggatac gggtagaccg gcgagggata gaagtaccac gtcggcccga
      481 ccacccacca ccagccgggg tgtccgccgt gcgggccgtg gacccagcgc ccgccgcgcc
      541 agacgtgcca gtcgtggtcg ccgaaatgcc ggatgtcgcc atgccagccc agcccgccgg
      601 gacgatggcc cccgtgcggc tgggcggcgg cgatggcggc cgtggcggcg gtcgcgatgg
      661 ccagcgccgc gcggcgggcg gatcgggatc gcgaaggagg gtgcgccatg gttcgctcct
      721 tgtctggcgc gcggcggcgt cggcccatcg tgcgcgttcg gatcgatcca tcgtgccgtc
      781 cgcaccgcgt gcgggattgc gcgcagtcaa ggacgggccg cgcacgttct tgcgcgtctt
      841 gttgatcgga cgcaatgcgc gcgcggcgac ccgcagcagc atgaccacgt caccgcagcc
      901 cgtgtgcgcc gcgggacgac cccggtgcga ggggacccat cgccgctgac ggcgacgcga
      961 tgcgtgttcg cgggatcgcc cggcggtcgt ccgctggaag ccgtggcggg cgatccggga
     1021 tgcggtggca cctgcacgtc gcctgcgcgc gtcgcggcgt ccgccccggg cgcgcaggcg
     1081 gcactcgagc caaggagtcc accatgaaga ccgacaagca gctcaaggac gacgtcgagg
     1141 ccgaactgga gtgggagccg tccatcgatg cggcccacgt cggcgtcgcc gcgaaggacg
     1201 gcgtcgtcac gctgaccggc cacctcaaca cgttcgcgga gaaggccgcc atcgagcgca
     1261 tcgcggcccg cgtcgccggg gtgcgggcgg tcgccgtcga gctcgacgtc aaggtcgccg
     1321 ccgagcatgt gcgcagcgac acggagatcg cggaggccgc gcgcaacgcg gtggcctgga
     1381 acgtcgtcgt gccgcgcgac aaggtgcagg tgaaggtcga gaagggctgg gtgaccctga
     1441 gcggcgagct ggactgggag taccagcgcg aggcggcgct gtcgtccgtc cgcgcgctca
     1501 agggcgtgcg cggcgtgacc agctcgctcg cgctcaagca gcgggtgctc tcgtcggaca
     1561 tcggggcgcg catcggcacg gcgctcgcgc gccacgccta ccgcgaggcc ggcgggatcc
     1621 acgtcggcgt cagcggctcg acggtgacgc tgcgcggcaa ggtcgactcg tgggccgagc
     1681 gcgccgccgc cgagggcgcg gcgtggtcgg cgccgggcgt caccgcggtc gtctccgacc
     1741 tgacgatcgg acgctgagca ccgcccgccg gtgcctgccc gcgtccaggg cgccgtccgc
     1801 ggccaggcgg gcggcccggt cactcgaccg tcacggtggt gccggtcgac agccgcgcgg
     1861 tcgaggcgtc ccaggattga ccggccgccg gccggtcggc gttggcgaag cgcaccgaga
     1921 agcggacatc gtcctgcgtc gccgcgaagg cgtccggcgc gctcacgaag acgtcgtaga
     1981 ggcccgtggc cgcgcccgcg gggatgggca gttgcagcac gatgcgcgtg gaggcggtgg
     2041 cctgcggcgc cagggtcgcg aggttgcccg cgctggcggc gagcgtctcg ccggtgtcgc
     2101 gccggcggat cgtcacgacg agcgcccgcg ccacgaacat gcgcgcccag ccgacgttcc
     2161 tcaggtcgac gtggacggcc agcgtgccgc cgcgggcgac cgccggcgcg ttcgacacgc
     2221 ggtcgagctg gatgcggtag cccatcgagg ccgcgacctg gtcgtagcag ccggcgttct
     2281 tccatccgtc gacgaccgac ggggcgtagt cgccgttcag ccaggccagg tggtagagcg
     2341 cgccttcgga caggatgtcg gcgcacgtgc cgcggatcgg cgtctcggcg ttctcgcagg
     2401 tctccccgcc gaacgagccc tcccgggtca ggagctgcgc gtaggcgcgc tggccgggtt
     2461 gcgtgtaggt gcccgtgtcc gacgggccgg ccaggaagca gtcgtcgtcc atgcccacct
     2521 gcgcgggggc gtcgccgtcg ggataccagg cctgcaggtc cgacggatag cggaagtcga
     2581 cctgcgtcgt cgggggcacg ttgcgcagca gcgcgtcacg gacgatcgcc cggttggcgg
     2641 cggccgtcgc gcaggaggtg ggcgccgatc cctcgtcgca ggagttgccg gactgcgacg
     2701 agtgccactc gccccaggcg ccgatgaagc cggcgcgcat gtaggggatg acgtcggcgt
     2761 tggcggccag gacgggccgc agctgctcga ggtggcgcgc gatccgggtc gagctcgcgt
     2821 cgaggcccga gccgctgaag tcgtaggcga acagcagggt ggtcttcatg ccggcggacc
     2881 tgacggcggc gaaacggtcc cccagcgtcg acaggaaggc cgcgctgagg tcgccggtgc
     2941 ggaatgccgc gagcgagacg gacgcgatca gcagccgccg gccgctcgcg tagaccgact
     3001 gcacgctggc gggatcgaag ccggccacga aatcgtcgcc cgccgacccg tagaagccgc
     3061 ggtcgggatt gggatagttc gcgccggaag cggcgaaggt ggcggtcgtg gtgcccggcg
     3121 gcgcacccgg cgcgggggag ggcgacgccg ggggcgcggg cgcctgcacc ggctggctgg
     3181 aggaggtgcc tccgctgcat gccgccgcca gcatcgcgca cgcggtcgcg aactggccca
     3241 ggcgcagcac ccggtccgtc acggcgcgcc gcttgtcgcc gcggcgtgcc gggcttgccg
     3301 cagagccgtc gtcgacggcc gcggccaggc gccagggagt cggaggtcga gccatccgcg
     3361 gatcctggcg cgcggatcgc cggcgccgat tgagtcggct cacgcgccct tcgcgttcct
     3421 tgaggtggag caagccccgc cgctcgccgc gtttccagaa tgcgacgact cgatcccggg
     3481 agcgcctgca tgacctatcc gaccgttctc ctgcacctcg acgacgacgc ccgggacgaa
     3541 gcccgcaacg aacaggccgt gcgcctcgcg ctgcacctcg gctgccattt gaacgggctg
     3601 gcctgccccc ggcccgtgcc cgcggccacc ggatttgcct cgtcgctggg cggcctcgac
     3661 gacgccttcg cgctggagct cgagcatgcg cgcgagcagg cccgcgtgcg cgagcgccgc
     3721 ttcctcggcc tgtgcga
//
LOCUS       DASVLN010000414         5566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_21372, whole genome shotgun sequence.
ACCESSION   DASVLN010000414 DASVLN010000000
VERSION     DASVLN010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21372"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..865)
                     /locus_tag="VF453_10300"
     CDS             complement(89..865)
                     /locus_tag="VF453_10300"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX7688090.1"
                     /translation="MSGAALEREVRFGDAVRAVDFISDIHLSETMPATARAWEDYLAA
                     TRADAVFMLGDLFEVWVGDDARSRPFEARCVEAIAALARRVPVFVQHGNRDFLLGAGF
                     AEATGARLLADPCALTAFGRTILATHGDELCVDDVPYQLFRRQVRDPAYQAQFLAQPL
                     EARLGIAAKMRAASRAGRDEPVTYADVDPALAKEWLGRAGTDTMIHGHTHRPGTQALD
                     GWTRHVLSDWDLDAADLARHRAEVLRWTAAGLERLPPSQA"
     gene            complement(862..1368)
                     /locus_tag="VF453_10305"
     CDS             complement(862..1368)
                     /locus_tag="VF453_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7688091.1"
                     /translation="MTKYVKMSTTAGDLRIELDDEKAPISVANFLSYVNKGFYDDTIF
                     HRVIKGFMIQGGGFAPGMKQKDTDAPIQNEAKNGLKNDKYTLAMARTSAPHSATAQFF
                     INTSGNNGFLNNDQAQDGWGYAVFGKVVEGQDVVDAIAGVKTGRKGFHDDVPLDDVKI
                     VKAEEVQA"
     gene            complement(1370..1945)
                     /locus_tag="VF453_10310"
     CDS             complement(1370..1945)
                     /locus_tag="VF453_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7688092.1"
                     /translation="MSIPHQTRRAAAVLAALAAFGAPAWAADKVHLQTSMGEIVIELD
                     AQHAPKSVANFEQYVKEGFYTNTVFHRVIPNFMVQGGGMTADLHEKPTHAPIPLEARN
                     GLHNTVGTVAMARTGDPNSATAQFFINVKDNGFLDAAQAADGNGYAVFGKVVAGMDVV
                     NKIRNVSTTTRGPFEDVPVQPVVILKATLEK"
     gene            2163..3563
                     /gene="cysS"
                     /locus_tag="VF453_10315"
     CDS             2163..3563
                     /gene="cysS"
                     /locus_tag="VF453_10315"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7688093.1"
                     /translation="MTLRLFNTLTGQHEEFKPIRAGHVGMYVCGMTVYDLCHMGHARM
                     MMSFDTVYRWLRASGYEVTYVRNITDIDDKIIKRALERRIPIRQLTDEMIAAMREDIG
                     ALGIAPPTHEPRATEHVGSMLAMIATLEDKGLAYRAPGGDVNLSVRKLPGYGRLSGKS
                     LDDLRAGAGLAERSDRADLAQSKVDPLDSVLWKASKPDEPEDAKWPSRFGPGRPGWHI
                     ECSAMCLDLLGKSFDLHGGGMDLQFPHHENELAQSEAANGTTFANYWLHNGFLNVDGE
                     KMSKSLGNFFTIRDVLKHYDGETLRFFMLRTHYRSPFNFADGLLDDARTALRRLYTAL
                     ASAPQSVEAAAIDWSEPRAAAFREAMDDDFNTPGALAVLFDLANELNRTRDVATASLL
                     RALGGVLGILQQPPQAFLQAGAALSEAEIQARIDARAAAKKAKDFAQSDRIRDELAAL
                     GITLKDGPQGTTWVKA"
     gene            3565..4221
                     /locus_tag="VF453_10320"
     CDS             3565..4221
                     /locus_tag="VF453_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX7688094.1"
                     /translation="MAPSNAAAARDVPGYWDEACRHLVKRDRVMKKLIPKFGDARLQS
                     NGDAFTTLARSIVGQQVSTRSAQGVWRRLVAAVDKRADDLLAPAAVLALTSEGLKAAG
                     LSARKVEYLTDLARHFRDGAVHVTQWHDMDDETIVEELVAIRGIGRWTAEMFLIFHLL
                     RPNVLPLDDPNLLRGISENYFSGEPVSRAEARELGEGWSPYRSVATWYLWRSLDPMTA
                     "
     gene            4271..5242
                     /locus_tag="VF453_10325"
     CDS             4271..5242
                     /locus_tag="VF453_10325"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX7688095.1"
                     /translation="MTKRHFLEFEQPIAELESKIEELRYVQNESAVDISEEIDRLDKK
                     SLQLTKDIYSSLQPWQVYQIARHPQRPQTLDYVNEVFTEFTEMHGDRSFADDAAIVGG
                     LARFNGQACMVVGHQRGYDTKDRQVRNFGMPRPEGYRKAQRLMKTAEKFGLPVFTFID
                     TMGAYPGIGAEERGQSEAIGASIFEMAQLEVPIVSTIIGEGGSGGALAIGVCDQLLML
                     QFSAYSVISPEGCASILWKTAERAAEAAEALGITAHRLKALGLVDKIVNEPVGGAHRD
                     PKAMSSSLKRALGDALRQVADLKPKDLLVRRQERLQAYGRFADTTDR"
     gene            5379..>5566
                     /locus_tag="VF453_10330"
     CDS             5379..>5566
                     /locus_tag="VF453_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7688096.1"
                     /translation="MAVSGGRDSLALLHATVRAARPLGVCVVALHVHHGLMDDADAWA
                     AGVGRECAQLADAGFDVR"
BASE COUNT          814 a         1965 c         1945 g          842 t
ORIGIN      
        1 cgtgtcgtcc gcgacggtca cgaggccggc tccgtgccgg ggcctcgggc ggcagtgcgc
       61 gccgccttcg ccgcggccgt ggagcgcctc atgcctgcga cggcggcagg cgctcgaggc
      121 cggccgccgt ccaccgcagc acttcggcgc ggtggcgcgc gaggtcggcc gcatcgaggt
      181 cccagtccga cagcacgtgc cgcgtccagc catccagggc ctgcgtgccg gggcgatggg
      241 tgtggccgtg gatcatcgtg tcggtgccgg cgcggccgag ccattccttc gcgagcgccg
      301 gatcgacgtc ggcgtaggtg acgggctcgt cccggcccgc gcggctggcg gcgcgcatct
      361 tggccgcgat gcccaggcgc gcctcgagcg gctgcgcgag gaactgcgcc tggtaggccg
      421 gatcgcgcac ctgccgtcgg aacagctggt agggcacgtc gtcgacgcac agctcgtcgc
      481 cgtgcgtggc gaggatcgtg cgtccgaacg ccgtgagcgc gcacgggtcg gccagcagcc
      541 gcgcgccggt ggcctcggcg aagccggcgc cgagcaggaa gtcgcggttg ccgtgctgca
      601 cgaacacggg cacccggcgc gcgagggcgg cgatcgcctc gacgcagcgg gcttcgaacg
      661 gccgcgagcg cgcgtcgtcg ccgacccata cctcgaacag gtcgccgagc atgaacacgg
      721 cgtccgcgcg cgtcgcggcg aggtagtcct cccacgcccg cgcggtggcg ggcatggtct
      781 cggacaggtg gatgtcggag atgaagtcga cggcgcgcac ggcgtcgccg aacctcacct
      841 cgcgttcgag ggctgcgccg ctcaagcctg gacttcttcc gccttgacga tcttcacgtc
      901 gtccagcggc acgtcgtcgt ggaaaccctt gcggccggtc ttcacgccgg cgatggcgtc
      961 gacgacgtcc tggccctcga cgaccttgcc gaagacggcg tagccccagc cgtcctgcgc
     1021 ctggtcgttg ttcaggaagc cgttgttgcc gctcgtgttg atgaagaact gcgccgtggc
     1081 ggagtgcggc gcggaggtgc gcgccatcgc cagcgtgtac ttgtcgttct tcaggccgtt
     1141 cttggcctcg ttctggatcg gggcgtcggt gtccttctgc ttcatgccgg gggcgaagcc
     1201 gccgccctgg atcatgaagc ccttgatcac gcggtggaag atggtgtcgt cgtagaagcc
     1261 cttgttcacg tacgacagga agttggcgac ggagatcggg gccttctcgt cgtcgagctc
     1321 gatgcgcagg tcacccgcgg tggtggacat cttcacgtac ttggtcattt cacttctcca
     1381 gggtggcttt gaggatcacg acgggctgga cggggacgtc ctcgaacgga ccccgggtcg
     1441 tggtcgagac gttgcggatc ttgttgacga cgtccatgcc cgcgacgacc ttgccgaaga
     1501 cggcgtagcc attgccgtcg gcggcctggg cggcgtcgag gaagccgttg tccttgacgt
     1561 tgatgaagaa ctgcgcggtc gcggagttcg ggtcgccggt gcgcgccatc gcgacggtgc
     1621 cgacggtgtt gtgcaggccg ttgcgcgcct ccagcgggat cggcgcgtgg gtgggcttct
     1681 cgtgcaggtc ggccgtcatg ccgccgccct ggaccatgaa gttcgggatc acgcggtgga
     1741 agacggtgtt cgtgtagaag ccttccttca cgtactgctc gaagttcgcg acggacttgg
     1801 gcgcgtgctg ggcgtcgagc tcgatcacga tctcgcccat cgaggtctgc aggtgcacct
     1861 tgtccgcggc ccacgcgggc gcgccgaacg cggccagggc ggccagcacg gccgcggcgc
     1921 ggcgggtttg gtgggggatg ctcatcaaag ggtctccttg gctatactgc ggcgattcta
     1981 gccagggccc gaccgcccgc cgttcccgcg cttgcccggc ccgtccggca gcgacgtcgc
     2041 ccgccctcgc gcaccggccg gtgcccgcgc ccggcacgcg cgacttcccg ccgttgcccg
     2101 atcatttcgc tcctgcttct cccggccggg ccacgagccc gcgcccgcca cgctttccgc
     2161 cgatgacgct gcgcctgttc aacaccctca ccgggcaaca cgaagagttc aaacccatcc
     2221 gggccggcca tgtcggcatg tacgtgtgcg gcatgaccgt gtacgacctg tgccacatgg
     2281 gccacgcgcg gatgatgatg tccttcgaca cggtgtaccg gtggctgcgc gcatcgggct
     2341 acgaggtcac gtacgtccgc aacatcacgg acatcgacga caagatcatc aagcgcgcgc
     2401 tcgagcgccg catcccgatc cgccagctca ccgacgagat gatcgcggcg atgcgcgagg
     2461 acatcggtgc gctgggcatc gcgccgccca cgcacgagcc gcgcgccacc gagcacgtcg
     2521 gctcgatgct cgccatgatc gcgacgctgg aggacaaggg cctcgcgtac cgcgcgccgg
     2581 gcggcgacgt gaacctgtcg gtgcgcaagc tgccgggcta tgggcggctg tcgggcaagt
     2641 cgctcgacga cctgcgcgcc ggcgccggcc tggccgagcg ctcggatcgc gccgacctcg
     2701 cgcagtccaa ggtcgacccg ctcgactccg tgctgtggaa ggcctccaag cccgacgagc
     2761 ccgaggacgc gaagtggccc agccggttcg gccccggccg tccgggctgg cacatcgagt
     2821 gctccgcgat gtgcctcgac ctgctgggca agagcttcga cctccacggc ggcggcatgg
     2881 acctgcagtt cccgcaccac gagaacgagc tcgcgcagag cgaggccgcc aacggcacca
     2941 cgttcgccaa ctactggctg cacaacggct tcctcaacgt cgacggcgag aagatgtcga
     3001 agtcgctcgg caacttcttc accatccgcg acgtcctcaa gcactacgac ggcgagacgc
     3061 tgcgcttctt catgctgcgc acgcactacc gcagcccgtt caacttcgcc gatgggctgc
     3121 tggacgacgc gcgcacggcg ctgcgccggc tctacacggc gctggcgtcg gccccgcagt
     3181 cggtcgaggc cgccgcgatc gactggagcg agccgcgtgc cgcggccttc cgcgaggcga
     3241 tggacgacga cttcaacacg cccggcgcgc tggcggtgct gttcgacctc gccaacgagc
     3301 tgaaccgcac ccgcgatgtc gcgaccgcgt cgctgctgcg tgcgcttggc ggcgtgctgg
     3361 gcatcctgca gcagccgccg caggccttcc tgcaggccgg cgccgcgctg tcggaggccg
     3421 agatccaggc ccgcatcgac gcccgcgccg ccgcgaagaa ggccaaggac ttcgcgcagt
     3481 ccgatcgcat ccgcgacgaa ctcgcggcgc tgggcatcac gctcaaggac ggcccgcagg
     3541 gcacgacctg ggtcaaggcc tgagatggcg ccctccaacg ccgccgccgc gcgcgacgtg
     3601 cccggctact gggacgaggc ctgtcgccac ctggtcaagc gcgaccgggt gatgaagaag
     3661 ctgatcccga agttcggcga cgcccgcctg cagagcaacg gcgacgcctt cacgacgctc
     3721 gcgcgctcca tcgtcgggca gcaggtgtcc acgcgctcgg cgcagggcgt ctggcgcagg
     3781 ctggtcgccg cggtcgacaa gcgcgccgac gacctgctcg cgcccgcggc cgtgctcgcg
     3841 ctgacgagcg agggcctgaa ggcggccggc ctgtcggcgc gcaaggtcga gtacctgacc
     3901 gacctcgcgc gccacttccg cgacggcgcc gtgcacgtca cgcagtggca cgacatggac
     3961 gacgagacca tcgtcgagga gctggtcgcg atccgcggca tcggccgctg gacggccgag
     4021 atgttcctga tcttccatct gctgcgcccg aacgtgctgc ctctggacga tccgaacctg
     4081 ctgcgcggaa tcagcgagaa ttacttcagc ggcgagcccg tctcccgcgc cgaggcccgc
     4141 gaactgggcg agggctggtc gccgtaccgc tccgtcgcga cgtggtacct gtggcgcagc
     4201 ctcgacccga tgacggcctg agggcccggg cacgcgaggt gcatgccgct tcccatcgag
     4261 gaccccaacg atgaccaaac gccacttcct ggaattcgag caaccgatcg ccgagctgga
     4321 atcgaagatc gaggagctgc gctacgtgca gaacgaatcg gccgtcgaca tctccgagga
     4381 gatcgaccgg ctcgacaaga agagcctgca gctcaccaag gacatctact cgagcctgca
     4441 gccctggcag gtctaccaga tcgcgcgcca cccgcagcgc ccgcagacgc tggactacgt
     4501 caacgaggtc ttcaccgagt tcacggagat gcacggcgac cgcagcttcg cggacgacgc
     4561 ggccatcgtc ggcggcctcg cgcgcttcaa cggccaggcc tgcatggtcg tcggccacca
     4621 gcgcggctac gacaccaagg accgccaggt gcgcaatttc ggcatgccgc gccccgaggg
     4681 ctaccgcaag gcgcagcgcc tgatgaagac ggccgagaag ttcggcctgc cggtcttcac
     4741 cttcatcgac acgatgggcg cgtacccggg catcggcgcc gaggagcgcg gccagtccga
     4801 ggcgatcggc gcgagcatct tcgagatggc gcagctcgag gtgcccatcg tctcgaccat
     4861 catcggcgag ggcggctccg gcggcgcgct ggccatcggc gtgtgcgacc agctgctgat
     4921 gctgcagttc tcggcctact ccgtgatctc gccggaaggc tgcgcgtcca tcctctggaa
     4981 gaccgccgag cgcgcggccg aggccgccga ggccctgggc atcactgcgc atcggttgaa
     5041 ggcgctgggc ctggtcgaca agatcgtcaa cgagcccgtc ggcggcgcgc accgcgatcc
     5101 gaaggcgatg agctcgtcgc tcaagcgcgc gctcggcgac gcgctgcgcc aggtcgccga
     5161 cctgaagccc aaggatctgc tcgtccgccg tcaggagcgc ctgcaggcct acggccggtt
     5221 tgccgacacg accgatcgct gagagcccga gcacgcgatt cgcccgcgcc aacccgcgtc
     5281 gcccgcccgt gcccgccgat cgagccgccc cgcgcgcgct tgaacgtcgc ggggatgcgg
     5341 cgggcgatgc acttttcgag ggggcgcgca cggtcgcggt ggccgtcagc ggcgggcgcg
     5401 attcgctcgc gctgctgcat gccaccgtgc gcgcggcgcg gccgctgggc gtctgcgtcg
     5461 tcgcgctgca tgtgcaccac gggctgatgg acgatgccga cgcgtgggcc gccggggtcg
     5521 ggcgcgaatg cgcgcagctg gccgacgcgg gcttcgacgt ccgctt
//
LOCUS       DASVLN010000415         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_290630, whole genome shotgun sequence.
ACCESSION   DASVLN010000415 DASVLN010000000
VERSION     DASVLN010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290630"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1266
                     /locus_tag="VF453_10335"
     CDS             136..1266
                     /locus_tag="VF453_10335"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7688097.1"
                     /translation="MSQPSTAVPEERRSAWAGFLRALTPRAIALTLLLSLAVAIAMNP
                     IFELPFSVLLGRTLFVGFCTLVARSAASLSSQRVVPPWVLQSIAVVLAAFLATLVVYL
                     LATGGDFEALVQHEGRVQGFVLISGTSTIVGLILALGAFERQRDSEAKAMGLELALQR
                     AELERHAADARLAVLAAQVEPHFLFNTLANVQALVETGSPRAPAVLRSLIAYLKATMP
                     RLDDGDCLLGREMALVRSYLELMHLRMPDRLRFDVAFDPSLQGFPCPPMALLTLVENA
                     VRHGIDPSETGGTIAVSAVRDGGTVRLSVRDDGAGMGEAPPSGTGLQNLRERLAAFYG
                     GAARLELTEVAPHGLLAEILVDGERARARVPAPGASTWKVAR"
     gene            1263..>1696
                     /locus_tag="VF453_10340"
     CDS             1263..>1696
                     /locus_tag="VF453_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7688098.1"
                     /translation="MTEARALIVDDEPLLRTHLAHHLAQLWPELEIAGEARNGREAVD
                     LAAAEKPDVVFLDVHMPGMNGVEAARLLGPDVQIVFVTAYEQYAVAAFEQGAIDYLVK
                     PFEVDRLAESVARLRRRLAERRRLSASPAAVSDTAPTDAPALP"
BASE COUNT          187 a          635 c          629 g          245 t
ORIGIN      
        1 atgatgcgca gcggctactg accggcggcg gggcgggggc ggaacggccg cttttcggcc
       61 cccgccaacc gccacccgtc gcaccggcgc cgccggcggc gggcgcgtgg ggcgtcgacg
      121 cggtatcgtg ccgccatgtc ccagccttcc accgccgttc ccgaggagcg ccgctccgcc
      181 tgggccggct tcctgcgcgc cctgacgccg cgcgcgatcg cgctgacgct gctgctgtcg
      241 ctcgcggtcg ccatcgccat gaacccgatc ttcgagctgc cgttcagcgt gctgctcggg
      301 cgcacgctct tcgtcggctt ctgcacgctc gtcgcccgca gcgccgcctc gctgtcgagc
      361 cagcgcgtcg tgccgccgtg ggtgctgcag tcgatcgccg tcgtgctcgc ggccttcctc
      421 gcgacgctgg tcgtctacct gctggccacc ggcggcgact tcgaggcgct ggtgcagcac
      481 gagggccgcg tgcagggctt cgtgctgatc tcgggcacct cgacgatcgt cggcctgatc
      541 ctcgcgctgg gggccttcga gcgccagcgc gactccgagg ccaaggcgat gggcctggag
      601 ctcgcgctgc agcgggcgga gctcgagcgc catgccgccg acgcccggct ggcggtgctc
      661 gccgcgcagg tcgagccgca tttcctgttc aacacgctgg ccaacgtgca ggcgctggtg
      721 gagaccggct cgccgcgtgc gccggcggtg ctgcgcagcc tgatcgcgta cctgaaggcg
      781 accatgccgc gcctggacga cggcgactgc ctgctcgggc gcgagatggc gctcgtgcgc
      841 agctacctcg agctgatgca cctgcgcatg cccgaccggc tgcgcttcga cgtcgcgttc
      901 gatccatcgc tgcagggctt cccgtgtccg ccgatggcgc tgctgaccct ggtcgagaac
      961 gcggtgcgcc acggcatcga cccgtcggag accggcggca cgatcgccgt ctcggccgtg
     1021 cgcgacggcg gcaccgtgcg gctgtcggtg cgcgacgacg gcgccggcat gggcgaggcg
     1081 ccgccctcgg gcacgggcct gcagaacctg cgcgagcgcc tggccgcgtt ctacggcggc
     1141 gcggcgcggc tcgagctcac cgaggtcgcg ccgcatgggc tgctggccga gatcctggtc
     1201 gacggcgagc gtgcccgcgc ccgcgtgccg gcgcccggcg cgagcacctg gaaggtcgcg
     1261 cgatgaccga ggcgcgcgcc ctcatcgtcg acgacgagcc gctgctgcgc acccatctcg
     1321 cgcaccacct ggcgcagctg tggccggagc tggagatcgc cggcgaggcc cgcaacggtc
     1381 gcgaggcggt cgacctggcg gccgccgaga agcccgacgt cgtcttcctg gacgtccaca
     1441 tgcccggcat gaacggcgtc gaggcggcgc ggctgctggg gccggacgtg cagatcgtct
     1501 tcgtgacggc ctacgagcag tacgcggtgg ccgcgttcga gcagggcgcc atcgactacc
     1561 tcgtcaagcc gttcgaggtc gaccggctgg ccgagagcgt cgcgcgcctg cggcggcggc
     1621 tcgcggagcg gcgccggctg tccgcgtcgc ccgcggcggt gtcggacacc gcgccgaccg
     1681 acgccccggc gttgcc
//
LOCUS       DASVLN010000416         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_342919, whole genome shotgun sequence.
ACCESSION   DASVLN010000416 DASVLN010000000
VERSION     DASVLN010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342919"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VF453_10345"
     CDS             complement(<1..329)
                     /locus_tag="VF453_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEX7688099.1"
                     /translation="MNAQYRKSLPGTQLDYFDARAAVEEIQAGAWDKLPYTSRVLAEQ
                     LVRRCEPELLKAALEQLVYRKRDLDFPWYPARVVCHDILGQTALVDLAGLRDAIADQG
                     GDPSKVNP"
     gene            complement(332..1483)
                     /gene="prpC"
                     /locus_tag="VF453_10350"
     CDS             complement(332..1483)
                     /gene="prpC"
                     /locus_tag="VF453_10350"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEX7688100.1"
                     /translation="MDAPTPAFKPKKSVALSGVAAGNTALCSVGRTGNDLHYRGYDIL
                     DVAEVCEFEEIAHLLVHGKLPTTAELAAYKAKLKAMRGLPACVKAALEALPAAAHPMD
                     VMRTGVSALGCVLPEKDDHNLPGARDIADRLMASLGSMLLYWYHWATNGKRIEVETDD
                     DSIGGHFLHLLHGAKPSPAWVRAMHTSLNLYAEHEFNASTFTARVIAGTGSDMYSAIA
                     GGIGALRGPKHGGANEVAFEIQKRYDNPDEAEADIRRRVEAKEVVIGFGHPVYTVSDP
                     RNVVIKGVAKRLSEAAGSTKMFDIAERLEGVMWEIKKMFPNLDWFSAVSYHMMGVPTA
                     MFTPLFVIARTAGWSAHVIEQRIDGKIIRPSANYVGPEDLKFVPLKDRK"
     gene            1620..>1996
                     /locus_tag="VF453_10355"
     CDS             1620..>1996
                     /locus_tag="VF453_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7688101.1"
                     /translation="MRKTFLGVKLKALREQRGLTQAALARQLGLSPSYLNQLENNQRP
                     LSVPVQLRLQSALGVDLQMLSDDDEARLTAQLQAVMAEAAGDTQVAQAELRALAQQMP
                     ALAQVVIRMHRRGRDAQVRLDSLA"
BASE COUNT          285 a          635 c          733 g          343 t
ORIGIN      
        1 gggttgacct tggacgggtc gccgccctgg tcggcgatgg cgtcgcgcag gccggccagg
       61 tcgaccagcg cggtctgccc gaggatgtcg tggcagacca cgcgcgccgg gtaccagggg
      121 aagtcgaggt cgcgcttgcg gtagaccagc tgttccagcg cggctttcag cagttccggc
      181 tcgcagcggc gcaccagctg ctcggccagc acgcgcgagg tgtacgggag tttgtcccag
      241 gcaccagcct ggatctcttc gacggccgca cgggcgtcga agtagtccag ttgtgtgccg
      301 ggcaggcttt tacggtattg ggcattcatg attatttacg gtccttgagc ggcacgaact
      361 tcaggtcttc gggacctacg tagttggcgc tcgggcggat gatcttgccg tcgatgcgct
      421 gctcgatgac gtgggcggac cagccggcgg tgcgggcgat cacgaacagc ggggtgaaca
      481 tggcggtggg cacgcccatc atgtggtagc tcacggcgct gaaccagtcg aggttgggga
      541 acatcttctt gatctcccac atcacgccct cgaggcgttc ggcgatgtcg aacatcttgg
      601 tcgagccggc cgcctcggac agccgcttgg cgacgccctt gatgacgacg ttgcgcgggt
      661 cgctgaccgt gtagaccggg tggccgaagc cgatcacgac ctccttggcc tcgacgcggc
      721 ggcggatgtc ggcctcggcc tcgtccgggt tgtcgtagcg cttctggatc tcgaacgcga
      781 cctcgttggc gccgccgtgc ttcgggccgc gcagcgcgcc gatgccgccg gcgatcgccg
      841 agtacatgtc gctgcccgtc cccgcgatca cgcgcgcggt gaaggtcgag gcgttgaact
      901 cgtgctcggc gtacaggttg agcgaggtgt gcatcgcgcg cacccaggcc ggcgagggct
      961 tggcgccgtg cagcaggtgc aggaagtggc cgccgatcga gtcgtcgtcg gtctcgacct
     1021 cgatgcgctt gccgttcgtg gcccagtggt accagtagag cagcatcgag ccgagcgagg
     1081 ccatcaggcg gtcggcgatg tcgcgcgcgc cgggcaggtt gtggtcgtcc ttctcgggca
     1141 gcacgcagcc cagcgccgag acgcccgtgc gcatcacgtc catcgggtgg gcggctgcgg
     1201 gcagtgcctc gagcgcggcc ttgacgcacg cgggcaggcc gcgcatggcc ttcagcttgg
     1261 ccttgtaggc ggccagctcg gccgtggtcg gcagcttgcc gtggacgagc aggtgcgcga
     1321 tctcctcgaa ttcgcagacc tcggcgacgt ccaggatgtc gtagccgcgg tagtgcaggt
     1381 cgttgccggt gcggccgacg ctgcacagcg cggtgttgcc ggccgcgacg cccgacaggg
     1441 cgacggactt cttcggcttg aaggcggggg tgggggcgtc catggcgggg aagactcgtt
     1501 atgagggtgg attcgcaatc ttcgcactcg gttcctcgtc gcgcaacgag agcttggcga
     1561 agatttgcga acgcggcgcc taaacttgcg atgtttgcga attcatcgga gcgtcatcca
     1621 tgcgcaagac cttcctgggc gtgaagctca aggcgttgcg ggagcagcgc ggcctcacgc
     1681 aggccgcgct cgcccggcag ctggggctgt cgccgagcta cctcaaccag ctcgagaaca
     1741 accagcgccc gctgagcgtg ccggtgcagc tgcggctgca gtcggcgctg ggcgtcgacc
     1801 tgcagatgct gtcggacgac gacgaggcgc gcctgaccgc gcagctgcag gcggtcatgg
     1861 ccgaggccgc cggcgacacg caggtcgccc aggccgagct gcgcgcgctg gcgcagcaga
     1921 tgccggcact cgcgcaggtc gtgatccgca tgcaccggcg cgggcgcgac gcgcaggtgc
     1981 gcctcgactc gctcgc
//
LOCUS       DASVLN010000417         4921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_129423, whole genome shotgun sequence.
ACCESSION   DASVLN010000417 DASVLN010000000
VERSION     DASVLN010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129423"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VF453_10360"
     CDS             complement(<1..266)
                     /locus_tag="VF453_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7688102.1"
                     /translation="MIDIEALLDERLRRADAVPLHRQIYERVRQAVVQGTLAPGQRVA
                     SARALASQLGLARGTVDAAYAQLSIEGYLEPMGQKGTRVSPSVRA"
     gene            358..1104
                     /locus_tag="VF453_10365"
     CDS             358..1104
                     /locus_tag="VF453_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7688103.1"
                     /translation="MRIFLAGASGVIGRRLVPMLVESGHDVHGGTRSERAAGLLRELG
                     ATPCVVDVFDRDALHAALRAVRPDVVVHQLTDLPPKLDPAHMVEGVARNARIRAEGTA
                     NLVAAALGAGCGRMVAQSIAWAYAPGGSPRRESDPLDLAADGLRATTIAGVAALETAV
                     LRTPGLVGAVLRYGRLYGPDTGADEPTDPVCVHVDAAAAAARLAVDLGAHGVFNVCEV
                     NDQVSPAKAIERLGWSDRMRLADAPAGSAR"
     gene            1191..1607
                     /locus_tag="VF453_10370"
     CDS             1191..1607
                     /locus_tag="VF453_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7688104.1"
                     /translation="MPHAVSEAIRAPGHLAARRAGAQARPATIVRPLSCTPLADAPGK
                     SLTTMLVEFPPDAFTGPHRHPGSVSAFVVAGAIRSQMAGAPAQVYTAGQTWFEPPMVL
                     HAFAENASATQPATLLATFVADDGCRQLVIPEHAGR"
     gene            complement(1981..2733)
                     /locus_tag="VF453_10375"
     CDS             complement(1981..2733)
                     /locus_tag="VF453_10375"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX7688105.1"
                     /translation="MTTSLQPLRGQVAIVTGASKGIGAGIALRLAAEGAAVVVNYARG
                     ADDAEAVVARITQAGGTAIAVQADLSRSDAAGPLVDAAIARFGRLDILVNNAGIYRID
                     SLETLTAESFDEHYRLNVRGLLLATQAAARVLPAGGVVVNISSGVAKSPSGSTHVYSS
                     SKGAVDVLTRSLGIELGPRGIRVVGVAPGFVETEGNAHIAADFAPALVAKTPLGRTGK
                     PVDIAAAVAFAVSRDAAWITGTTIDAAGGLVF"
     gene            2864..3757
                     /locus_tag="VF453_10380"
     CDS             2864..3757
                     /locus_tag="VF453_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688106.1"
                     /translation="MSPDLLPAIAAFARVAHHGSFTRAAAEMGVSPSALSQTLRTLEA
                     RLGVRLLERSTRHVRPTEIGRRFLDEAQPGLASLAAAVEGLDEARARPAGLLRLNVSQ
                     SAVKSVLRPHLAAFADAYPDVVVELHCDNALLDLVAGGFDAGIRLGENLADGVVAVPL
                     GPRQRLATVAAPSYLAGRKPPRTPDDLREHRCIQVRLQGGLYRWEYAHKGRDFAIETQ
                     GPLVTNDGEVMMTALHAGLGIACPIEAMVADDIAAGRLVPLLKAWWPTFPGYYLYHST
                     RVHVPRKLRVFIDFMTARCNR"
     gene            complement(3770..4786)
                     /locus_tag="VF453_10385"
     CDS             complement(3770..4786)
                     /locus_tag="VF453_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX7688107.1"
                     /translation="MTYETNRRIVLAARPHGRPAASDFRFETVPVPEPAEGQVLLRTR
                     FLSLDPYMRNLMEEAGPGYAPPVALGATMPGGTVSRVVASRHPHHRVGDLVLANAGWQ
                     DYALSDGADLLPVGDPARPSLALGGLGMPGFTAYVGLLDIGQPRPGETVVVAAATGAV
                     GGVVGQIAKVKGARVVGIAGGADKARFAVEELGFDACLDRRAPDFAQRLAAACPDGID
                     VYFENVGGEVLDAVLPLLNAGARVPVCGFISHYNAAGDDGNRARLLATVLARRVRLQG
                     FIILDHYAAHFEAFRRDMLAWLAEGRVRLREDLVDGLEQAPAALAGLLEGRNFGKVVV
                     RVAD"
BASE COUNT          652 a         1827 c         1795 g          647 t
ORIGIN      
        1 gcgcggacac tcggcgagac gcgcgtgccc ttctgcccca taggctcgag gtagccctcg
       61 atcgacagct gcgcataggc ggcgtcgacc gtgccccggg cgagtccgag ctggctcgcg
      121 agcgcgcgcg cggaggcgac gcgctgcccc ggcgcgagcg tgccctgcac caccgcctgg
      181 cgcacgcgct cgtagatctg ccgatgcagc ggcacggcgt cggcgcgccg gagccgctcg
      241 tcgaggagcg cctcgatgtc gatcatggcc cagtccgaag cattgttctt ggcactttcc
      301 ggtgggccat cctactccta cagtgagcgc attgccccac tcctccagga gaacaacatg
      361 cgaatcttcc ttgccggcgc cagcggcgtg atcggccgcc gcctcgtgcc gatgctggtc
      421 gagtccggcc acgacgtcca cggcggcacg cgctccgagc gcgcagcagg cctgctgcgc
      481 gagctcggcg cgacgccctg cgtggtcgac gtcttcgacc gcgacgccct gcatgcggcg
      541 ctgcgggccg tgcggcctga cgtcgtcgtc caccagctca ccgacctgcc acccaagctc
      601 gatccggcgc acatggtcga gggcgtcgcg cgcaacgcgc gcatccgcgc cgagggcacg
      661 gccaacctgg tcgcggccgc gcttggcgcg ggctgcggac ggatggtggc gcagagcatc
      721 gcgtgggcgt atgcgccggg cggctcgcct cggcgcgagt cggatccgct ggatctcgcc
      781 gcggatggcc tgcgggccac gacgatcgcg ggcgttgccg ccctggagac ggccgtcctg
      841 cgcacgcccg ggctggtcgg cgcggtgctg cgctatggcc gcctctacgg cccggacacg
      901 ggcgccgacg agccgaccga cccggtgtgc gtgcatgtcg acgccgcggc cgcggcggcg
      961 cgcctggccg tggacctcgg cgcgcacggc gtcttcaacg tctgcgaggt gaacgaccag
     1021 gtctcgccgg ccaaggccat cgagcgcctg ggctggtccg accgcatgcg cctcgccgac
     1081 gcccccgccg gaagtgcgcg atgaccaccg ccgcacgacg caggacgcgg tccacctggc
     1141 ctctggcagc gctggcgctg gccgccggcg cagtcctcgt cacgcgcccg atgccccatg
     1201 ctgtatccga ggcgatccgg gcgcccggcc atctggccgc ccggcgggcc ggcgcccagg
     1261 cgcggccggc gacgatcgtg cggccgctgt cgtgcacgcc gctggccgac gcgcccggca
     1321 agagcctgac gacgatgctg gtggagtttc cgccggacgc attcacgggg ccgcaccggc
     1381 atccgggctc cgtgagcgcg ttcgtggtgg cgggcgcgat ccgctcgcag atggccggcg
     1441 cgccggcgca ggtctacacc gcgggacaga cgtggttcga gccgccgatg gtgctgcacg
     1501 cattcgccga gaacgccagc gcaacgcagc cagcgaccct cctcgccact ttcgtcgccg
     1561 acgacggctg ccgccagctc gtcatccccg agcacgcggg ccgttagcag cggatgatcg
     1621 ccatggcccg cacgatccag gcgatcgaag ggccgcgccc cgtcaccgcc gccaccgcgg
     1681 gacacgccac gctcgtcccc ttcctgcgac tcgcggcatg caacttcggc gtcgcgaccg
     1741 cgttggaacg gcacgctgac gctgatgctc gcgcgcagtg cagcccggca gtcgcgggga
     1801 caggcgcgct ggtcgtcgtc gcgtcgcacg tgtcgtgcga gcgcccgcgg gcacgcggcg
     1861 gtcacgacgc gggatgtccc cattcgacca gcgtcccgtg gcggccgtcc gccgactcga
     1921 cgagcgcatg ccgtcgcacc gtccaggcga ccgccggcgg cgcatcgcca cgacgcgaac
     1981 tcagaacacc agcccgccgg ccgcatcgat cgtcgtgccc gtgatccacg ccgcatcccg
     2041 cgacaccgcg aacgccaccg cggcggcgat gtcgaccggc ttgccggtgc ggccgagcgg
     2101 ggtcttcgcg accagcgcgg gcgcgaagtc ggcggcgata tgggcgttgc cctcggtctc
     2161 gacgaagccc ggcgccacgc ccaccacgcg gatgccgcgc gggcccagct cgatcccgag
     2221 cgagcgcgtg agcacgtcga ccgcgccctt gctgctggag tagacgtggg tcgaccccga
     2281 cgggctcttc gcgacgcccg agctgatgtt gacgacgaca ccgcccgccg gcagcacgcg
     2341 cgccgccgcc tgcgtcgcga gcagcaatcc gcgcacgttg aggcggtagt gctcgtcgaa
     2401 gctctccgcc gtgagggtct ccagcgagtc gatgcgatag atgccggcgt tgttgacgag
     2461 gatgtccagg cggccgaagc gcgcgatcgc cgcatcgacc agcggcccgg ccgcgtcgga
     2521 ccgggagagg tcggcctgca ccgcgatcgc cgtgccgccg gcttgcgtga tgcgcgcgac
     2581 gacggcctcg gcgtcgtccg cgccgcgcgc atagttgacg acgacggccg cgccctcggc
     2641 ggccaggcgc agcgcgatgc ccgcgccgat gcccttggag gcgccggtga cgatggcgac
     2701 ctggccgcgc agcggctgga gggacgtggt catggagcga gcctttcgag aggtgggcac
     2761 cgcgtcggag gacacggcat ggctcgcacg ttagcgacga agctttgcgg gaaaaacggg
     2821 gttgtgcgca gctccgtttg aagccatcct tcataatcgg ccgatgtcgc ccgacctgct
     2881 gcccgccatc gccgcgttcg cgcgcgtcgc ccatcacggc agcttcacgc gcgcggcggc
     2941 ggagatgggc gtgtccccgt ccgcgctgtc gcagacgctg cgcacgctcg aggcccggct
     3001 aggggtgcgg ctgctggagc gctcgacgcg ccacgtccgc ccgaccgaga tcgggcggcg
     3061 cttcctcgac gaggcccaac ccggcctcgc ctcgctggcg gcggcggtcg agggcctcga
     3121 cgaggcgcgc gcacggcccg cggggctgct gcggctgaac gtatcccaat cggcggtgaa
     3181 gtcggtgctg cggccgcacc tcgcggcgtt cgcggacgcc tatcccgacg tcgtcgtgga
     3241 gctgcattgc gacaacgcgc tgctcgatct cgtggccggc ggcttcgacg ccggcatccg
     3301 gctgggggag aaccttgccg acggcgtggt cgccgtgccg ctcggcccgc gccaacgcct
     3361 ggccaccgtc gccgcgccga gctacctcgc gggccgcaag ccgccgcgca cgcccgacga
     3421 cctgcgcgag catcggtgca tccaggtgcg gctgcagggc gggctgtacc gctgggagta
     3481 tgcgcacaag ggccgcgact tcgccatcga gacccagggg ccgctcgtga ccaacgacgg
     3541 cgaggtcatg atgacggcgc tgcacgcggg cctcgggatc gcctgcccca tcgaggcgat
     3601 ggtggccgac gacatcgccg ccggccgcct cgtgccgctg ctgaaggcct ggtggccgac
     3661 gttcccgggc tactacctct accactcgac ccgcgtgcat gtgccgcgca agctgcgcgt
     3721 cttcatcgac ttcatgacgg cgcgctgcaa ccgctgaggc cgcgcccggt cagtcggcca
     3781 cgcgcacgac caccttgccg aagttgcgcc cctccagcag gccggccagc gcagcgggcg
     3841 cctgctcgag gccgtcgacg aggtcctcgc gcagtcgcac gcggccctcg gccagccacg
     3901 ccagcatgtc gcggcggaag gcctcgaagt gcgcggcgta gtggtcgagg atgatgaagc
     3961 cctgcagtcg cacgcgccgg gcgagcaccg tcgccagcag ccgcgcgcgg ttgccgtcgt
     4021 cgcccgccgc gttgtagtgc gagatgaagc cgcacaccgg cacgcgcgcg ccggcattga
     4081 gcagcggcag caccgcgtcc agcacctcgc cgccgacgtt ctcgaagtag acgtcgatgc
     4141 catcggggca ggccgcggcg aggcgctgcg cgaagtcggg cgcgcggcga tcgaggcagg
     4201 cgtcgaagcc gagctcctcg accgcgaagc gggccttgtc cgcgccgcct gcgatgccca
     4261 cgacgcgcgc gcctttcacc ttcgcgatct gcccgacgac gccgccgacc gcgcccgtgg
     4321 ccgcggcgac gaccacggtc tcgcccggcc tgggctgccc gatgtccagc agcccgacgt
     4381 aggccgtgaa tcccggcatg cccaggccgc cgagcgcaag cgacgggcgc gccggatccc
     4441 ccacggggag gaggtcggcg ccgtcggaca gcgcgtagtc ctgccagccc gcgttcgcga
     4501 ggacgaggtc gccgacgcgg tgatgcggat gtcgcgatgc cacgacgcgg ctgacggtgc
     4561 cgcccggcat cgtcgcgccg agcgcgaccg gcggcgcgta gccggggccg gcttcctcca
     4621 tcaggttgcg catgtacgga tcgagcgaga ggaatcgcgt gcgcagcagc acctgccctt
     4681 cggcgggctc gggcacgggc acggtctcga agcggaagtc gctcgcggcg ggacggccgt
     4741 gcggacgcgc ggcgaggacg atgcggcggt tggtttcgta cgtcatgggg tatgagcttc
     4801 gcagcgttgg aggttcaggc tcgagtggcg cgcggcccgt ggcgcagcac gagcagcgtg
     4861 ccgggcagga cggcggcgat cgcgaaggcg gggccgccgc gcgcgccgat gcggtcgacg
     4921 a
//
LOCUS       DASVLN010000418         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_290684, whole genome shotgun sequence.
ACCESSION   DASVLN010000418 DASVLN010000000
VERSION     DASVLN010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290684"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..1240
                     /locus_tag="VF453_10390"
     CDS             314..1240
                     /locus_tag="VF453_10390"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7688108.1"
                     /translation="MCVSSRVRSGAACLALAALAACGGKSSSDNSEVAAKVNGDPITV
                     AQISAALPRQRASQADVADAAGRAMLDRLIETDILAQKAVKAKLDQDPRVVAQIESAR
                     RDILARRFVEEVAETGAKPTGQQVQKFFDERPNLFGQRKLFTLQRLDIQVPDDRRTEV
                     DAHVSSLKTSAELTDWLKAQNLKFTSRQEQAASEQIPAQLLDKLATLKDGESTVMPSP
                     SGVMAVTRVSAVAAPKTLADARPAIEQFLSMQGKREAIMNLQKSVKDGAQIEYLGRFA
                     PNAAPAAAPAAPASVSANSAATAASQNPPGQK"
     gene            1237..>2040
                     /locus_tag="VF453_10395"
     CDS             1237..>2040
                     /locus_tag="VF453_10395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7688109.1"
                     /translation="MKLRARVVLSLAVVGAAVALAGCGKNKYDATPVLATVNGENVTQ
                     GQVDYVRSQFLAAHPGASAPENAQILKGLVEQRLITQKAEKEKLDRNPGVLQAIEETR
                     KDALARFYIDELAAKVPKPGADDIKQYYDGHPLNFAQRAIYTLQKVDARVPADQLQAV
                     VQAAQATSSADEAAAVVKAKATASNVTLSPQPAESLGGLLPRIAQLKPGQTVCVPMPG
                     GLSAVTLVSVQPAPLTLAQATGTIENLVWNQRKREAIQAEAKTLHADAKI"
BASE COUNT          335 a          748 c          687 g          270 t
ORIGIN      
        1 ggcgcgatta tggacgaccg ctccggcgcc cggatcgtgc gtgccggagt gcgacggcgg
       61 gaaccgcgcc ggtcgcggac cgaccacgac gccagcgtcg ggaggccgcg gacgttgcaa
      121 tttcgcacgg tcgcccctgc aggcttgggg ggcgctgcga ggttccccgg atcgtcgcgg
      181 gaatgccatg ggttagtctc tacaatcgcg cgtttgccgc catgcggccc gggcaatgac
      241 ttccgaaggc gtgggcccgc atcgacactg agccgccagg ctcgagaagg gcttgccgtc
      301 catgtttcgt tccgtgtgtg tgtcttcccg cgtgcgttcc ggcgccgcct gcctggccct
      361 ggccgccctg gccgcctgcg gcgggaagag ctcctccgac aactccgagg tcgccgccaa
      421 ggtcaacggc gacccgatca ccgtcgccca gatctccgcc gcccttccgc gccagcgcgc
      481 ctcgcaggcg gacgtcgccg atgccgccgg ccgcgcgatg ctcgatcgcc tgatcgagac
      541 ggacatcctg gcccagaagg ccgtcaaggc gaagctggac caggatccgc gcgtcgtcgc
      601 gcagatcgaa tcggcccggc gcgacatcct cgcccgccgc ttcgtcgagg aggtggccga
      661 gaccggcgcc aagccgaccg ggcagcaggt gcagaagttc ttcgacgagc gccccaacct
      721 gttcgggcag cgcaagctgt tcacgctgca gcgcctggac atccaggtgc cggacgatcg
      781 ccgcaccgag gtggacgccc acgtctcgtc gctgaagacg tccgcggagc tcacggactg
      841 gctcaaggcg cagaacctga agttcaccag ccgccaggag caggccgcct ccgagcagat
      901 cccggcgcag ctgctggaca agctcgccac gctcaaggac ggcgaatcca ccgtcatgcc
      961 ctcgccgtcc ggcgtgatgg ccgtcacgcg cgtttccgcg gtggccgcgc ccaagacgct
     1021 cgccgacgcc cgtcccgcca tcgagcagtt cctctcgatg cagggcaagc gcgaggcgat
     1081 catgaacctg cagaagtccg tcaaggacgg tgcgcagatc gagtacctgg gccgcttcgc
     1141 gcccaacgcc gcaccggccg ccgcgccggc cgcgcccgcg tccgtttccg ccaactccgc
     1201 cgccaccgcg gcgtcccaga atcccccagg ccaaaaatga agcttcgcgc acgagttgtc
     1261 ctttcgctgg cggtcgtcgg cgccgccgtc gccctggccg gttgcggcaa gaacaagtac
     1321 gacgccacgc cggtgctcgc caccgtcaac ggcgagaacg tcacccaggg ccaggtcgac
     1381 tacgtgcgct cgcagttcct cgcggcgcat ccgggcgcgt ccgcgccgga gaacgcgcag
     1441 atcctcaagg gcctcgtgga gcagcgcctg atcacgcaga aggcggagaa ggagaagctc
     1501 gatcgcaacc cgggcgtgct gcaggcgatc gaggagacgc gcaaggatgc gctcgcgcgc
     1561 ttctacatcg acgagctcgc ggccaaggtg cccaagcccg gtgccgacga catcaagcag
     1621 tactacgacg gccacccgct gaacttcgcc cagcgcgcga tctacacgct gcagaaggtc
     1681 gacgcccgcg tgccggcgga ccagctgcag gccgtggtgc aggcggcgca ggcgacgagc
     1741 tccgccgacg aggccgctgc cgtggtcaag gccaaggcga ccgcctccaa cgtgacgctg
     1801 tcgccgcagc ccgcggaatc gctcggcggc ctgctgccgc ggatcgccca gctgaagccc
     1861 ggccagaccg tgtgcgtgcc gatgcccggc ggcctgagcg cggtgacgct ggtgagcgtg
     1921 cagcccgcgc ccctgacgct ggcccaggcg accggcacga tcgagaacct ggtgtggaac
     1981 cagcgcaagc gcgaggccat ccaagccgag gccaagacgc tgcacgccga tgcgaagatc
//
LOCUS       DASVLN010000419         3622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_343041, whole genome shotgun sequence.
ACCESSION   DASVLN010000419 DASVLN010000000
VERSION     DASVLN010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343041"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF453_10400"
     CDS             complement(<1..204)
                     /locus_tag="VF453_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688110.1"
                     /translation="MRPRDDDRGAALVAAGATLLGLSVLADSGMEHYRGSFHNKAMVL
                     PLVAAGLTIGATVLARRERASRAV"
     gene            complement(388..1503)
                     /locus_tag="VF453_10405"
     CDS             complement(388..1503)
                     /locus_tag="VF453_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX7688111.1"
                     /translation="MAAEPALPVEAVRAAAYRIPTDGPERDGTFAWDSTTLVVVQVHA
                     GGRYGMGYTYADAAAASLATGALATAIAGRDAMDVEACWQAMQRAVRNIGRSGVAACA
                     ISALDAALWDAKARALGVSLATLLGRCRDAVPVYGSGGFTNYDDARLREQLGGWVERD
                     GCRHVKMKIGTDAADDPRRVAVARSAIGSAELFVDANGGYGVRQALRLLQACADAGLR
                     WFEEPVSSDDVEGLRRVREACGAAVDVAAGEYVFTLDDAAHLLEGRAVDVLQADVTRC
                     GGITGFLKIAALCEAHHLELSGHCAPSLHLPVAAAVARLRHLEWFHDHVRIEHALFDG
                     APRVREGAIRPDASAPGNGLAFKAIDARPYLVQGDIA"
     gene            complement(1493..3289)
                     /locus_tag="VF453_10410"
     CDS             complement(1493..3289)
                     /locus_tag="VF453_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX7688112.1"
                     /translation="MAKTVGDFVVERLHEWGVRLLFGYPGDGINGVFGGLNRAGGKIR
                     FIQARHEEMAAFMASAYAKFTGEMGACIATSGPGASHLVTGLYDARLDHMPVLAIVGQ
                     QARDALGGHYQQELDLPALFKDVAGSFVQQASMPAQVRHIVDRAVRVAMAERRVAAII
                     LPNDLQEEKYEDPPRAHGTLHSGVGYAKPVVVPARMDLQRAAEVLNAGRKVALLVGAG
                     ALHATDEVIAVADKLQAGAAKALLGKAALADDLPWVTGSIGLLGTEPSWKLMNECDTL
                     LMIGSGFPYSEFLPKEGQARAVQIDLAPDMLSLRYPMEVNLVGDAAETLRALLPLLQD
                     KADTSWRDDIAGWMSSWWKTLEERAMQPANPVNPQRVAWELSPRLPANVIVTSDSGSC
                     ANWFARDLRMRRGMMASLSGGLASMGAAVPYAIAAKFAHPDRPVVALVGDGAMQMNNM
                     AELITVSKYWKEWADPRWIVCVFNNEDLNQVTWEQRVMEGDPKFDASQQIPNVPYHRF
                     GELIGLRGLYVDDPERLGAAWEEALSSDRPVVLEVKTDPEVPPLPPHITFKQASNFAK
                     ALAKGDPREGGMIAGAMRQVLGAILPGHHDGR"
     gene            3425..>3622
                     /locus_tag="VF453_10415"
     CDS             3425..>3622
                     /locus_tag="VF453_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX7688113.1"
                     /translation="MDRPLFQDRADAGRAVARLLGRYAGRSDVLVLALPRGGVPVAFE
                     VAAALRAPLDVLVVRKLGVPGH"
BASE COUNT          478 a         1330 c         1312 g          502 t
ORIGIN      
        1 gaccgcgcgt gacgcacgct cgcgccgcgc gagcaccgtc gcgccgatgg tcaggccggc
       61 ggccaccagc ggcagcacca tcgccttgtt gtggaacgag ccgcggtagt gctccatgcc
      121 gctgtcggcg aggacggaca gcccgagcag ggtcgcgccg gcggcgacga gcgccgcgcc
      181 gcggtcgtcg tcgcgaggac gcatcgcgaa agctccggtt tcgcggcctg cggcgcgagc
      241 ccctacgggc ggccgggcgg gggtcatgag gaagcctccg tgctgcgcac tgcggcgcga
      301 gcccctacgg gcggccgggc gggggtcatg aggacgcctc cgtgctgcgc actgcggtgc
      361 gagcccctac gggcggccgg gcggggctca tgcgatgtct ccttggacga ggtagggccg
      421 cgcgtcgatg gccttgaagg cgagcccgtt gccgggcgcc gacgcgtcgg gccggatcgc
      481 gccttcccgc acgcgcggtg cgccgtcgaa cagggcgtgc tcgatgcgca cgtggtcgtg
      541 gaaccattcg aggtgccgca gccgcgcgac ggcggcggcg acgggcaggt gcagcgacgg
      601 cgcgcagtgg ccggacagct cgaggtgatg cgcctcgcac agcgcggcga tcttcaggaa
      661 gccggtgatc ccgccgcagc gcgtgacgtc ggcctgcagc acgtcgaccg ctcggccctc
      721 gagcaggtgc gcggcgtcgt cgagcgtgaa gacgtactcg ccggcggcga cgtcgaccgc
      781 ggcgccacag gcctcgcgga cgcggcgcag gccctcgacg tcgtcggacg agaccggttc
      841 ctcgaaccag cgcaggcccg cgtcggcgca ggcctgcagc agccgcagcg cctggcgcac
      901 gccgtagccg ccgttggcgt cgacgaacag ctcggccgac ccgatggccg agcgcgcgac
      961 ggccacgcgt cgcgggtcgt cggcggcgtc ggtgccgatc ttcatcttca catggcggca
     1021 gccgtcgcgt tcgacccagc cgccgagctg ttcgcgcagg cgcgcgtcgt cgtagttcgt
     1081 gaagccgccg ctgccgtaca cgggcaccgc gtcgcggcag cggccgagca gcgtcgcgag
     1141 cgacacgccc agcgcgcgcg ccttggcgtc ccacagcgca gcgtcgagcg cggagatcgc
     1201 gcacgcggcg acgcccgagc ggccgatgtt gcgcaccgcg cgctgcatcg cctgccagca
     1261 ggcctcgacg tccatcgcgt cgcggccggc gatcgccgtc gcgagcgcgc cggtggccag
     1321 cgaggcggcg gcggcgtcgg catacgtgta ccccatcccg tatcgaccgc ccgcgtgcac
     1381 ctgcacgacg acgagcgtgg tcgagtccca ggcgaacgtg ccgtcgcgct cgggaccgtc
     1441 ggtcgggatc cggtaggccg cggctcgcac ggcctcgacc ggcaacgcgg gctcagcggc
     1501 catcgtggtg cccgggcagg atcgcgccca gcacctggcg catcgcgccg gcgatcatgc
     1561 cgccctcgcg cgggtcgccc ttggccagcg ccttggcgaa gttgctcgcc tgcttgaacg
     1621 tgatgtgcgg cggcagcggc ggcacctcgg ggtcggtctt gacctcgagc acgacgggcc
     1681 ggtcgctgga aagcgcttcc tcccacgccg cgccgagccg ctcggggtcg tcgacgtaga
     1741 ggccacgcag cccgatcagc tcgccgaagc ggtggtaggg cacgttgggg atctgctgcg
     1801 acgcgtcgaa cttcgggtcg ccttccatga cgcgctgctc ccaggtgacc tggttgaggt
     1861 cctcgttgtt gaagacgcag acgatccagc gcgggtcggc ccattccttc cagtacttgg
     1921 agacggtgat gagctccgcc atgttgttca tctgcatggc gccgtcgccc accagcgcga
     1981 ccaccggccg gtcgggatgg gcgaacttcg cggcgatcgc gtacggcacc gccgcgccca
     2041 tcgacgcgag gccgcccgac agcgaggcca tcatcccgcg tcgcatgcgc aggtcgcgcg
     2101 cgaaccagtt cgcgcacgag ccggagtcgc tcgtcacgat gacgttggcg ggcaggcgcg
     2161 gcgagagctc ccacgcgacg cgctgcgggt tgaccgggtt ggcgggctgc atcgcgcgct
     2221 cctcgagcgt cttccaccag ctcgacatcc agccggcgat gtcgtcgcgc caggacgtgt
     2281 ccgccttgtc ctgcagcagg ggcagcagcg cgcgcagcgt ctcggcggca tcgccgacga
     2341 ggttgacctc catcggatag cgcagcgaga gcatgtccgg cgcgaggtcg atctgcacgg
     2401 cgcgcgcctg gccctccttg ggcaggaact ccgagtacgg gaagcccgag ccgatcatca
     2461 gcagcgtgtc gcactcgttc atcagcttcc agctcggctc ggtgccgagc aggccgatcg
     2521 agcccgtgac ccaggggagg tcgtcggcca gagcggcctt gcccagcagc gccttcgcgg
     2581 cgccggcctg cagcttgtcg gccaccgcga tcacctcgtc ggtggcgtgc agcgccccgg
     2641 cgccgacgag cagcgcgacc ttcctgcccg cgttgagcac ctcggccgcg cgctgcaggt
     2701 ccatgcgcgc cggcacgacc accggcttcg cataccccac ccccgaatgc agcgtgccgt
     2761 gcgcgcgcgg cggatcctcg tacttttctt cctgcaagtc gttcggcagg atgatcgccg
     2821 ccacgcggcg ctcggccatc gccacccgaa cggcgcggtc gacgatgtgg cgcacctgcg
     2881 ccggcatcga ggcctgctgc acgaaggagc cggccacgtc cttgaacagc gcgggcagat
     2941 cgagctcctg ctggtagtgc ccgccgagcg cgtcgcgcgc ctgctggccg acgatcgcga
     3001 gcaccggcat gtggtcgagc cgcgcgtcgt acaggcccgt cacgaggtgc gacgcgccgg
     3061 ggcccgaggt cgcgatgcac gcgcccatct cgccggtgaa cttcgcgtac gccgaggcca
     3121 tgaacgcggc catctcctcg tgcctcgcct ggatgaagcg gatcttgccg ccggcacggt
     3181 tgagcccgcc gaacacgccg ttgatgccat cgcccgggta gccgaacagg aggcgcacgc
     3241 cccactcgtg gagtcgctcg accacgaaat cgccgacggt cttggccatg ctctcgttcc
     3301 tcgtcgctgg aaacgccgga catccggccc tccggcacgg caattcccac gcccccgaca
     3361 gcaggatcgg cctgccgcgg ccgcctgcaa cggccgagtg gcacgccgaa tgccgcgcat
     3421 cggcatggac cgtccgctgt tccaggatcg cgccgacgcc ggccgtgcgg tcgcgcgcct
     3481 gctcgggcga tacgcgggcc gctccgacgt gctggtgctc gccctgccgc gcggtggcgt
     3541 gccggtcgcg ttcgaggtcg ccgcggcgct gcgggcgccg ctggacgtgc tggtcgtgcg
     3601 caagctcggc gtccccggcc ac
//
LOCUS       DASVLN010000420         5826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_506246, whole genome shotgun sequence.
ACCESSION   DASVLN010000420 DASVLN010000000
VERSION     DASVLN010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506246"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..2159)
                     /locus_tag="VF453_10420"
     CDS             complement(279..2159)
                     /locus_tag="VF453_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX7688114.1"
                     /translation="MSSKERGAGLAAMTLGALGVVYGDIGTSPLYTLHEIFLPADGVG
                     VPLDTHHLVGAISSIFWGLMLVVTLKYVILILRADNNGEGGAMALTALASRAGGTERR
                     RMLIMMVGMFGATLFFGDSVITPAVTVMGAVEGLEVAAPALKAYVVPLSVVVLVVLFA
                     AQRFGTGAVGKSFGPIIVVWFTGMGVVGLIHVLQAPAILTALNPIEAVRFLLERGWHV
                     FAALGAIVLALTGAEALYADMGHFGKTPIRLAWSVLVLPSLALNYMGQGALLMREPAA
                     IANPFYHLFPQAWVIPVVVLATMAAVIASQAVISGAFSMTKQAIQLGFLPRMEVIYTS
                     AREAGQIYLPRVNWLLLAAVTISVIGFGSASKLTAAYGIAVTVTMLIDTVLTYFVVRY
                     GWGFPAWIAVGATGFFLGLDVLLVTACSLKFLQGGWFPLTLGLAIFAVMSTWKRGREL
                     LLENITRDDPDLVPFITALAADDIARTPRTAVYAVANPGTVPQAMLHNLKHYQVLHET
                     NVILTVVFHEVPWVPFAERVQVEPLARGFWRVTINYGFKNTPDIPQALTLCRGYGLEI
                     EELTTSYFLSREVVVPTRGDGMAHWRERLFAAMTRNAGSVVEFFRLPNNAVIELGTRV
                     QI"
     gene            2326..3294
                     /gene="corA"
                     /locus_tag="VF453_10425"
     CDS             2326..3294
                     /gene="corA"
                     /locus_tag="VF453_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX7688115.1"
                     /translation="MLINCVAYDNGAKLADIPIAQISDYLARKGCFVWVALKDASDEE
                     LDAMQREFGLHELAIEDARHGHQRPKVEEYGDTLFAVVHTVEMTPTDQIHVGELNLFV
                     GLNFVLSVRNRSRQNLLQVRERAEREPQLLRHGPAFVFYALMDAVVDRYFPIIDTLET
                     DLENIEDQIFEPGTSQANIEQLYALKRKIGVVRHAVVPLIDSVHKLFSGRVPAVCEHN
                     REYFRDVFDHLLRMNGSLDTLRDTIGTAIQVNLAMVSIEESVVNKRLAAWAGIFAVIS
                     AFAGIWGMNFKAMPELEWKYGYPAALAVIAAVCVILYRQFRKSGWL"
     gene            complement(3308..4645)
                     /locus_tag="VF453_10430"
     CDS             complement(3308..4645)
                     /locus_tag="VF453_10430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7688116.1"
                     /translation="MSARVLRRLLILLLALTAAALLWNEFGMGTTMVIDGRSSLRIGG
                     VDDRVSDGGHSVASVARDGSALEMDCDVKAGYQWPYCELEVVFRDAPAGIDLSRYDSM
                     RLWLSASGPEGRQRVRVYVRNFDPKYSRPGVGESEKVNQLVYEPSAYPSGYEVPLSRF
                     TVSQWWIDEHPMTLDLEAPDFRNVTRMSFSTGPVVVPGMHHVRVDRIELHGKLVSSST
                     FRLGVIAVWMALVFGWLVVDTVTTRGQLHLSAASQHSLQRLNESLRRETRHLAEAARS
                     DPLTGVLNRKGLADELMRLVRAGGERTFPMTLVFIDIDHFKQVNDRCGHDVGDQVIRG
                     LAELVRSAVQRDDLFARWGGEEFLLVFRATGGTEGRAIAERLRERIAAAQWPGGLAIT
                     CSFGVAEWHRDEDLNDGIKRADEAMYRAKQNGRDRVEAELERDAAPASEPSPV"
     gene            complement(4912..5826)
                     /locus_tag="VF453_10435"
     CDS             complement(4912..5826)
                     /locus_tag="VF453_10435"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX7688117.1"
                     /translation="MLLAQVSGELVRDRRNERRWRRVMRTAWFLLALAFVWGIVAAQR
                     GHVAAPSGPHTALIEVRGEIAAGSEASAENIVSALQSAFEEKNAKAIVLRFNSPGGSP
                     VQAGIVYDEIKRLKALHHKKVYAVCEEMCASGAYYMAVGADEIFVDKASIVGSIGVLM
                     DGFGFTGLMDKLGVERRLITAGENKGMLDPFSPLQPKQRAYAQAMIDQIHQQFIAVVR
                     EGRGARLHETPDTFSGLFWNGQDAVSQGLADHLGNLDYVARDVVHAEDVIDYTLQENV
                     AERLAKRFGASIGAGAVKAMQAAGVSLH"
BASE COUNT          912 a         2041 c         2040 g          833 t
ORIGIN      
        1 ttgatggtcc atcacgatcg ctccaactgg gcgcccgtcg catgggtgtg gctgaccgag
       61 gcccgccttc tcgcttcgcg attggcgcag gcgtagatca tttcagctac accctctcag
      121 aaggtgtgaa tgaatccagc gtgtccgagc gggttgccag atcgcgtcca atccaggcgg
      181 aaagcgcaga cgtagctcgg gctacggcga gcatttccaa cgacgagtgg gcgcgatctg
      241 gcggcacgag cgggcatggt ggattcgttc acaccttctc agatctgcac gcgcgtgccc
      301 agctcgatga cggcgttgtt gggcagccgg aagaactcca cgacgctgcc ggcgttgcgc
      361 gtcatcgccg cgaacaggcg ctcgcgccaa tgggccatgc cgtcgccgcg cgtgggtacc
      421 acgacctcgc ggctgaggaa gtagctggtc gtgagctcct cgatctccag cccgtagccg
      481 cggcacagcg tgagcgcctg cgggatgtcg ggcgtgttct tgaagccgta gttgatcgtc
      541 acgcgccaga agccgcgcgc gagcggctcg acctgcaccc gctcggcgaa cggcacccag
      601 ggcacctcgt ggaagacgac ggtgaggatg acgttggtct cgtgcagcac ctggtagtgc
      661 ttgaggttgt gcagcatcgc ctgcggcacc gtgcccgggt tggcgaccgc gtagaccgcc
      721 gtgcggggcg tgcgcgcgat gtcgtcggcg gccagggccg tgatgaaggg cacgaggtcg
      781 gggtcgtcgc gggtgatgtt ctcgagcagc agctcgcgcc cgcgcttcca ggtcgacatc
      841 accgcgaaga tggcgagccc cagcgtgagc gggaaccagc cgccctgcag gaacttcagc
      901 gagcacgcgg tgacgagcag cacgtccagg ccgaggaaga agccggtcgc gccgaccgcg
      961 atccacgccg ggaagcccca gccgtagcgc acgacgaagt aggtcagcac ggtgtcgatg
     1021 agcatcgtca cggtcaccgc gatgccgtag gcggccgtca gcttggaggc gctgccgaag
     1081 ccgatcaccg agatcgtcac cgccgcgagc agcagccagt tgacgcgcgg caggtagatc
     1141 tggccggcct cgcgcgccga cgtgtagatc acctccatgc gcggcaggaa gcccagctgg
     1201 atcgcctgct tggtcatgga gaacgcgccc gagatgaccg cctgcgacgc gatcacggcg
     1261 gccatcgtcg ccagcacgac gaccgggatc acccaggcct gcgggaacag gtggtagaac
     1321 gggttggcga tcgccgccgg ctcgcgcatc agcagcgcgc cctggcccat gtagttcagc
     1381 gccagcgaag gcagcacgag cacgctccag gcgagccgga tcggcgtctt gccgaagtgg
     1441 cccatgtcgg cgtaaagcgc ctccgcgccg gtcagcgcca gcacgatcgc ccccagcgcc
     1501 gcgaacacgt gccagccgcg ctcgagcagg aagcgcaccg cctcgatcgg gttgagcgcg
     1561 gtgaggatgg ccggcgcctg cagcacatgg atcagcccga ccacgcccat gccggtgaac
     1621 cagaccacga tgatcgggcc gaagctcttg cccaccgcgc cggtgccgaa gcgctgggcc
     1681 gcgaacagca ccaccagcac gaccaccgac agcggcacca cgtaggcctt gagcgccggc
     1741 gcggcgacct ccaggccctc gaccgcgccc atcaccgtga ccgccggcgt gatgacgctg
     1801 tcgccgaaga acagcgtggc gccgaacatc cccaccatca tgatgagcat gcgccgccgc
     1861 tccgtgccgc cggcgcgcga agcgagcgcg gtcagcgcca tcgcgccgcc ctcgccgttg
     1921 ttgtcggcgc gcaggatgag gatcacgtac ttcagcgtga ccaccagcat caggccccag
     1981 aagatcgacg agatcgcgcc gacgaggtgg tgggtgtcca gcggcacgcc gacgccgtcg
     2041 gcgggcagga agatctcgtg cagggtgtaa agcgggctgg tgccgatgtc gccgtagacg
     2101 acgcccagcg caccgagggt catcgccgcg aggccggcgc ctcgttcctt gctgctcatg
     2161 aagggttccg gttgtccggg cggcattgtc gccgaaggcg tcggcgccgc cgggcgcggc
     2221 ccgcaccggg cccgcccgtc acgcggccgt cacgttcgcg tggacgcggg tagagtcggc
     2281 gttcccgccg gcgcccgcgc gccgggccac catgccgccc gccccatgct gatcaactgc
     2341 gtcgcctacg acaacggagc caagctcgcc gacattccga tcgcgcagat cagcgactac
     2401 ctcgcgcgca agggctgctt cgtctgggtc gcgctgaagg acgcgagcga cgaggagctc
     2461 gacgccatgc agcgcgagtt cggcctgcac gagctcgcga tcgaggacgc ccgccacggc
     2521 caccagcggc ccaaggtcga ggagtacggc gacacgctgt tcgccgtcgt gcacacggtg
     2581 gagatgaccc ccacggacca gatccacgtc ggcgagctga acctgttcgt cggcctgaac
     2641 ttcgtgctgt cggtgcgcaa ccgcagccgc cagaacctgc tgcaggtgcg cgagcgcgcc
     2701 gagcgcgagc cgcagctgct gcgccacggc ccggccttcg tgttctacgc gctgatggac
     2761 gcggtggtcg accgctactt cccgatcatc gacacgctgg agaccgacct cgagaacatc
     2821 gaggaccaga tcttcgagcc cggcacctcg caggccaaca tcgagcagct gtacgcgctc
     2881 aagcgcaaga tcggcgtcgt gcgccatgcg gtggtgccgc tgatcgactc cgtgcacaag
     2941 ctgttcagcg gccgcgtgcc ggccgtctgc gagcacaacc gcgagtactt ccgcgacgtc
     3001 ttcgaccacc tgctgcgcat gaacggctcg ctcgacaccc tgcgcgacac catcggcacg
     3061 gcgatccagg tcaacctcgc gatggtgtcg atcgaggagt cggtcgtgaa caagcggctc
     3121 gcggcctggg cggggatctt cgccgtgatc agcgccttcg cgggcatctg gggcatgaac
     3181 ttcaaggcca tgcccgagct ggagtggaag tacggctatc ccgcggcgct ggccgtcatc
     3241 gcggcggtgt gcgtgatcct gtaccggcag ttcaggaaat ccggctggct gtagccggac
     3301 gccgcactca gaccggcgac ggctccgacg ccggcgccgc gtcgcgctcc agctcggcct
     3361 cgacgcggtc gcgcccgttc tgcttggcgc ggtacatcgc ctcgtcggcg cgcttgatgc
     3421 cgtcgttgag atcctcgtcg cggtgccact cggccacgcc gaagctgcag gtgatcgcaa
     3481 gcccgcccgg ccactgcgcc gcggcgatgc gctcgcgcag gcgctcggcg atcgcgcggc
     3541 cctcggtgcc gccggtcgcg cggaagacga ggaggaactc ctcgccgccc cagcgcgcga
     3601 acaggtcgtc gcgctgcacc gccgagcgca cgagctcggc caggccgcgg atcacctggt
     3661 cgccgacgtc gtggccgcag cggtcgttga cctgcttgaa gtggtcgatg tcgatgaaga
     3721 ccagggtcat cgggaaggtg cgctcgccgc cggcgcgcac gaggcgcatc agttcgtcgg
     3781 ccaggccctt gcggttgagc acgccggtga gcgggtcgct gcgcgccgcc tcggcgaggt
     3841 ggcgcgtctc gcggcgcagc gattcgttga gccgctgcag cgagtgctgg ctggccgcgc
     3901 tcaggtgcag ctggccgcgc gtcgtcacgg tgtcgacgac gagccagccg aacaccagcg
     3961 ccatccacac cgcgatcacg ccgaggcgga acgtcgagct cgagaccagc ttgccgtgca
     4021 gctcgatgcg gtcgacgcgg acgtggtgca tgccgggcac gaccaccggc ccggtcgaga
     4081 acgacatccg cgtgacgttg cggaagtcgg gcgcctcgag gtccagcgtc atcgggtgct
     4141 cgtcgatcca ccactgcgag accgtgaagc ggctcaacgg cacctcgtag ccggacggat
     4201 acgccgacgg ctcgtagacg agctggttga ccttctcgct ctcgcccaca ccgggcctgg
     4261 aatacttcgg gtcgaagttg cggacgtaga cgcgcacgcg ctgccggccc tcgggcccgc
     4321 tggcgctgag ccacaggcgc atgctgtcgt agcggctcag gtcgatgccg gcgggcgcgt
     4381 cgcggaagac gacctcgagc tcgcagtacg gccactggta gccggccttg acgtcgcagt
     4441 ccatctccag cgccgagccg tcgcgcgcga ccgaggcgac gctgtggccg ccgtcgctca
     4501 cgcgatcgtc gacgccgccg atccgcaacg aggagcggcc gtcgatcacc atcgtcgtgc
     4561 ccatgccgaa ctcgttccaa agcagcgccg ccgccgtgag cgcgagcagg aggatcagca
     4621 gtctgcggag gacgcgggcg gacatggcgc gggaacggga gcggtcggac gggcggcgac
     4681 gatcgggaat cggcgcgcct gccgtcatct tgaggcgtgc ggcggttccg gcgcgccgac
     4741 gcggggcgac atccgtgccg gagttcacga agacgccggg ggccggccct cgcaaggcgg
     4801 gccgcgcccg gcggcgcacg cggacgcccg tccgctggcc ggtcgtcgct cgccgaccgc
     4861 cgatcgccga ccgcgggcac gggcccggcc gccggacggg gcgcggcgcg atcagtgcag
     4921 cgagaccccc gccgcctgca tcgccttgac cgcgcccgcg ccgatggacg cgccgaagcg
     4981 cttggccagg cgctcggcga cgttctcctg cagcgtgtag tcgatcacgt cctcggcatg
     5041 cacgacgtcg cgcgcgacgt agtcgaggtt gccgaggtgg tcggccaggc cctgcgagac
     5101 ggcgtcctgg ccgttccaga acaggccgga gaaggtgtcg ggcgtctcgt gcaggcgcgc
     5161 cccgcggccc tcgcgcacga cggcgatgaa ctgctggtgg atctggtcga tcatcgcctg
     5221 cgcgtaggcg cgctgcttgg gctgcagcgg cgagaacggg tcgagcatgc ccttgttctc
     5281 gcccgcggtg atgagccggc gctcgacccc cagcttgtcc atcaggccgg tgaagccgaa
     5341 gccgtccatc agcacgccga tcgagccgac gatgctcgcc ttgtcgacga agatctcgtc
     5401 cgcgccgacg gccatgtagt acgcgccgga ggcgcacatc tcctcgcaca ccgcgtagac
     5461 cttcttgtgg tgcagggcct tcagccgctt gatctcgtcg tagacgatgc cggcctgcac
     5521 cgggctgccg ccgggcgagt tgaagcgcag cacgatggcc ttggcgttct tctcctcgaa
     5581 ggcgctctgc agggccgaga cgatgttctc ggcgctcgcc tcggagccgg cggcgatctc
     5641 gccgcgcacc tcgatcagcg ccgtgtgcgg cccgctgggc gccgccacgt ggccgcgctg
     5701 cgccgccacg atgccccaca cgaaggccag cgcgagcagg aaccacgccg tgcgcatcac
     5761 gcggcgccag cggcgctcgt tgcggcggtc gcgcaccagc tcgccagaga cctgcgcgag
     5821 cagcag
//
LOCUS       DASVLN010000421         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_75192, whole genome shotgun sequence.
ACCESSION   DASVLN010000421 DASVLN010000000
VERSION     DASVLN010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75192"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..598)
                     /locus_tag="VF453_10440"
     CDS             complement(35..598)
                     /locus_tag="VF453_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7688118.1"
                     /translation="MATAPRKTAAGSRGRTTATRSANPDAIALLKDDHKRVSALFDEY
                     EKSRSITKKQALVARICQELTIHAMIEEEIFYPAVRAALKDRELVPEALVEHATLKEF
                     IAKVEGRTPDGDMFDAHIQVMGEYVKHHVKEEQNEMFPKAKRSKLDMKELGARLAARK
                     EELKASPGSLPKQPNAVEMLSEDSAPA"
     gene            672..1454
                     /locus_tag="VF453_10445"
     CDS             672..1454
                     /locus_tag="VF453_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX7688119.1"
                     /translation="MDASAAAASRAAGIPLAVGERPHDAPAAMSDYELYYWPMPFRGQ
                     FIRAILAHAGKRWDEYAADDIAATMHAAPDAQPVPFMGPPMLHDRRAGVWLAQMPAIA
                     YYLAETLDLLPRKVALQALALKVVNDANDVIDELTLDGGRQMWTPRRWKEHVPRLRRW
                     MAIWESTGRRHGLAADAGCLLGGARVGVADPVTATLWDTMSERFPVIGELLDDEAPRV
                     AGLTRRVCALPSIANLRAQTRARFGDAYCGGRIERSMRQVIR"
     gene            complement(1512..>1762)
                     /locus_tag="VF453_10450"
     CDS             complement(1512..>1762)
                     /locus_tag="VF453_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX7688120.1"
                     /translation="PRPVVVEGDVELLTLSGSIGAGGSHLHLSVADARGRVVGGHAAY
                     GCVVRTTAEVLLALAPGWAFAREADPATGWSELVVRRR"
BASE COUNT          220 a          653 c          616 g          273 t
ORIGIN      
        1 tcgcgtcgca gctgacgcct cgccggcgcc gccgtcaggc cggcgcgctg tcctcggaca
       61 gcatctcgac cgcgttgggc tgcttcggca gcgagcccgg cgaggccttg agctcctcct
      121 tgcgggcggc caggcgcgcc ccgagctcct tcatgtccag cttcgagcgc ttcgccttgg
      181 ggaacatctc gttctgttcc tccttgacgt ggtgcttgac gtactcgccc atcacctgga
      241 tgtgcgcgtc gaacatgtcg ccgtccggcg tgcggccttc gaccttggcg atgaactcct
      301 tgagcgtcgc gtgctcgacg agcgcttccg gcacgagctc gcgatccttg agcgccgcgc
      361 gcacggccgg atagaagatc tcctcctcga tcatcgcgtg gatcgtcagc tcctggcaga
      421 tcctggcgac cagggcctgc ttcttggtga tcgagcggct cttctcgtac tcgtcgaaca
      481 gcgcgctcac gcgcttgtgg tcgtccttca gcagtgcgat cgcgtcggga ttcgccgacc
      541 gcgtcgccgt cgtgcggccg cgcgatccgg cggccgtctt cctgggtgca gttgccatcg
      601 tgtgctcctc gtgcgtgtga ctcgttgggt tgcgcctcgg cgcgtcgaca cagatggcaa
      661 gcggcaggcc cgtggacgcc tccgcggccg cggccagccg cgctgccggc atcccgcttg
      721 ccgtcggcga gcgcccgcac gacgcccctg ccgccatgtc cgactacgag ctctactact
      781 ggccgatgcc tttccgcggc cagttcatcc gcgcgatcct cgcccacgcc ggcaagcgct
      841 gggacgaata cgccgcggac gacatcgccg cgacgatgca cgccgccccc gacgcgcagc
      901 cggtgccgtt catggggccg ccgatgctgc atgaccggcg cgccggcgtc tggctcgcgc
      961 agatgccggc gatcgcgtac tacctggccg agacgctcga cctgctgccg cgcaaggtcg
     1021 cgctgcaggc gctggcgctg aaggtcgtca acgacgcgaa cgacgtcatc gacgaactca
     1081 cgctggacgg cggccgccag atgtggacgc cgcgacgctg gaaggagcac gtgccgcggc
     1141 tgcggcgctg gatggcgatc tgggaatcca ccggccgccg ccacggcctc gcggccgatg
     1201 ccggctgcct gctgggcggc gcgcgcgtgg gcgtcgccga ccccgtgacc gcgacgctgt
     1261 gggacacgat gtccgagcgc ttcccggtca tcggcgagct gctggacgac gaggcgccgc
     1321 gcgtcgccgg cctgacgcgg cgcgtctgcg ccctgccctc gatcgccaac ctgcgcgcgc
     1381 agacgcgggc gcgcttcggc gacgcctact gcggagggcg gatcgagcgc tcgatgcggc
     1441 aggtgatccg ctgagccggc gtcgggacga aggacgcggc gcgtgccgcg gacgtcgatg
     1501 cggcctcgcc ttcagcgccg gcgcacgacg agctcgctcc agcccgtcgc cggatcggcc
     1561 tcgcgcgcga aggcccagcc cggtgcaagc gcaagcagca cctcggccgt cgtgcggacg
     1621 acgcagccgt aggccgcgtg gccgccgacg acgcgaccgc gggcgtcggc gacgctcagg
     1681 tgcaggtgcg agccgcctgc gccgatcgag ccggacagcg tcagcagctc gacgtcgccc
     1741 tcgaccacga ccggcctggg cg
//
LOCUS       DASVLN010000422         3776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_75224, whole genome shotgun sequence.
ACCESSION   DASVLN010000422 DASVLN010000000
VERSION     DASVLN010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75224"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..1318)
                     /locus_tag="VF453_10455"
     CDS             complement(290..1318)
                     /locus_tag="VF453_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7688121.1"
                     /translation="MTTRKRFLNSMAGSALALAAGGFARPARAQAAPIVIRFSHVVAP
                     DTPKGIGAQRFTELAEQRSSGRLKVEVYPNSQLYKDKEELEALQLGSVQMLAPSLAKF
                     GPLGVREFEVFDLPYLMKDEAAFHAMVDGPLGAELLRKLEPKGIKGLAYWDNGFHVMT
                     ANRPLHAVADFKGLKMRIQSSKVLDAQMRALGAIPQVMAASELYQGLQSGVVDGAEGV
                     PSGIYTQKLHEVQKHLTISNHGHLAYAVIVNRKFWDGMPPDLRAVVESALRDATALVN
                     RNANAENAAALERIRASGRTTVYALAPAEVEAWKRALMPVHHEAAGRVGQATIDAAYK
                     AAGFVAPA"
     gene            complement(1393..2568)
                     /locus_tag="VF453_10460"
     CDS             complement(1393..2568)
                     /locus_tag="VF453_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7688122.1"
                     /translation="MSKRMRASLAIAGGLVCACAQAQDGGPAAPASGSPLTAHVAGSS
                     VTLYGLIEATASDVDHATATNGKLVSFQAPWFSGSRLGFTGTHPFDDDLAGIFKLEAE
                     YVPQTGAMGTSNVIFNRDAWAGFLSPTWGKLTFGRQDALGRDISAIYGDPYGAAAVGT
                     DEGGYTNQNNFKALVNYGGSATGSRMDNGVVWKKAWRSGLVAGLGFQFGGTAGNQQRG
                     QTKSAALGYNLGALHVAGFYTAANVASHRDRAASLGANYQWDLVRVGGGWYRYTGGQS
                     PRLQGRRDNAYTAHVKFTPTTRWDFTLGYLRIDASDAALDAGGNVLNPFADVSAATKI
                     ASGARDTIYGAAMLHLDKASDVYVAADHLKLTEGYRVSSAPGSMHQNELAVGLRYKF"
     gene            complement(2763..3116)
                     /locus_tag="VF453_10465"
     CDS             complement(2763..3116)
                     /locus_tag="VF453_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEX7688123.1"
                     /translation="MPPSPMPFPDRPPASARLCIRGVTREGRAFRPADWAERLAGALG
                     AFLLEPQARRAGACWSPLCVPRVVEGVKCVVVDPALARLQPLAWDFVTGFARDNALEV
                     RDVHLPMDHEAPARG"
     gene            3356..>3776
                     /locus_tag="VF453_10470"
     CDS             3356..>3776
                     /locus_tag="VF453_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7688124.1"
                     /translation="MNHWHRARVCVALAACLAAGVARGNERVVLMVGGIDKQIYLPAR
                     LAQRLDYFAAEGVDVELRSEESGVGARDQLLLGTVDGVIGFYDHTIDLQAKGKLVESV
                     VQFARAPGEALLVSGRSAERVRTLADLRGRPVGVTGLG"
BASE COUNT          505 a         1330 c         1391 g          550 t
ORIGIN      
        1 ccgccgacgc caacgcgccc atcgtcgccg cgctcaccgg gcgcagcggc gagccgctcg
       61 tcgcgccggt ggtgcgggtc gccgccggcc gcgagcacgg catctccggc atcgtcgacg
      121 cgcacggctt caaggtgcgc atcgcgcccg agcgcctgta tgcgaaggcg cggctgtagc
      181 gcgccatcgc gggtgagaaa gcgagagccg acccacgcgg gcatgcacca ccggctcccc
      241 catgaaaccg catggcccgc gcggctctcg gcgcggctct cgtgaaacct caggcaggcg
      301 cgacgaagcc cgcggccttg tacgcggcgt cgatcgtggc ctgcccgacg cggccggccg
      361 cctcgtgatg caccggcatc agcgcgcgct tccaggcctc gacctccgct ggcgccagcg
      421 cgtagacggt cgtgcggccc gaggcgcgga tgcgctccag ggccgcggcg ttctccgcgt
      481 tggcgttgcg gttgacgagg gcggtggcgt cgcgcaacgc ggactcgacg acggcgcgca
      541 ggtcgggcgg catgccgtcc cagaacttcc ggttgacgat cacggcgtac gcgaggtggc
      601 cgtggttgga gatcgtcagg tgcttctgga cctcgtggag cttctgggtg tagatgcccg
      661 acggcacccc ttccgcgccg tcgacgacgc ccgactgcag gccctggtag agctcgctgg
      721 ccgccatcac ctgcgggatc gcgcccagcg cgcgcatctg cgcgtcgagc accttggagg
      781 actggatgcg catcttcagg cccttgaagt cggcgaccgc gtgcagcggc cggttggccg
      841 tcatcacgtg gaagccgttg tcccagtagg cgaggccctt gatgcccttg ggctcgagct
      901 tgcgcagcag ctccgcaccc agcggcccgt cgaccatcgc gtggaacgcg gcctcgtcct
      961 tcatcaggta gggcaggtcg aagacctcga actcccggac gcccagcggg ccgaacttgg
     1021 ccagcgacgg cgcgagcatc tgcaccgagc cgagctgcag cgcctcgagc tcctccttgt
     1081 ccttgtacaa ctggctgttg ggatagacct cgaccttcag gcggccgctg gagcgctgct
     1141 ccgcgagctc cgtgaagcgc tgcgcaccga tgcccttcgg ggtatccggc gcgaccacgt
     1201 ggctgaaccg gatcacgatc ggcgcggcct gcgcgcgcgc cgggcgcgcg aagccgccgg
     1261 cagccagggc cagcgccgag ccggccatcg agttgaggaa tcgctttctc gtcgtcatcg
     1321 cttgtctccc ggtggcgcgg ggacggcgca accgcggcgc cgggcgcgcc gcggtccgcc
     1381 ggcctcgcag gctcagaact tgtagcgcag gcctacggcc agctcgttct ggtgcatcga
     1441 gccgggcgcg ctggacaccc ggtagccctc ggtgagcttc aggtgatccg cggcgacgta
     1501 gacgtcgctg gccttgtcga ggtgcagcat cgcggcgccg tagatcgtgt cgcgggcgcc
     1561 cgaggcgatc ttggtcgcgg cgctgacgtc ggcgaacggg ttgagcacgt tgccgcccgc
     1621 gtccaacgcg gcgtcgctcg cgtcgatgcg caggtagccg agcgtgaagt cccagcgcgt
     1681 cgtcggcgtg aacttcacgt gcgccgtgta cgcgttgtcg cgccgaccct gcagcctggg
     1741 cgactggccg ccggtgtagc ggtaccagcc gccgccgacg cgcacgagat cccactggta
     1801 gttcgcgccg agcgacgccg cgcggtcgcg atggctggcg acgttggccg cggtgtagaa
     1861 acccgcgacg tgcagcgcgc cgaggttgta gccgagcgcc gccgacttcg tctgcccgcg
     1921 ctgctggttg cccgcggtac cgccgaactg gaagccgagg ccggcgacga ggccggagcg
     1981 ccacgccttc ttccacacga cgccgttgtc catgcgcgat ccggtggcgc tgccgccgta
     2041 gttcacgagc gccttgaagt tgttctggtt cgtgtagccg ccctcgtcgg tgccgaccgc
     2101 ggccgcgccg tacgggtcgc cgtagatcgc ggagatgtcg cgcccgagcg cgtcctggcg
     2161 gccgaaagtc agcttgcccc aggtcgggct caggaagccg gcccaggcgt cgcggttgaa
     2221 gatcacgttg gacgttccca tcgcgcccgt ctgcggcacg tactcggcct cgagcttgaa
     2281 gatgccggcg aggtcgtcgt cgaacggatg cgtgccggtg aagcccagcc ggctgccgct
     2341 gaaccacggc gcctggaagc tcaccagctt gccgttggtc gcggtggcgt ggtcgacgtc
     2401 gctggccgtc gcctcgatga ggccgtacaa ggtcaccgac gagcccgcca cgtgcgccgt
     2461 cagcgggctg ccggaggccg gcgcggccgg tccgccgtcc tgcgcctgcg cgcacgcgca
     2521 gaccagccct cctgcaatgg cgagactcgc cctcatcctc ttgctcatgt catcgtctcc
     2581 tgttggttga acgtcgcgcc gcgcggcccg catggcggtg cgttgggcga cgggagcgaa
     2641 gactaggaaa cgacgccttt cgaacggctt tcgcgccgcc gcgattcggg taatcgcgtg
     2701 cagggccgca tgcagggccg caacaccgcg cgcggcgaag gccgttcgta agccgagctg
     2761 cgttatccgc gtgcgggcgc ctcgtggtcc atgggcaggt ggacgtcgcg gacctcgagc
     2821 gcgttgtcgc gcgcgaagcc ggtgacgaag tcccaggcga gcggctgcag ccgcgccagg
     2881 gcggggtcga cgacgacgca cttgacgccc tcgaccaccc gcggcacgca cagcggcgac
     2941 cagcaggccc ccgcgcgccg cgcctgcggc tcgaggagga acgcgcccaa cgcgccggcc
     3001 aggcgttcgg cccagtcggc cggccggaac gcgcggccct cgcgcgtgac gccgcggatg
     3061 cacaggcggg ccgaggccgg cggtcggtcg gggaacggca tcgggctggg cggcatcgag
     3121 gcggacgtgg aggactcgcg cacgcatcga ccggcgcgcg gcgcgcggcg gtctgcgggc
     3181 cgcgcgagcc taggcaagtc ctgctttcgc gcggctttcg tcacgcttcg cgtcggcatg
     3241 cacgccacgc cgggcgcggc cgggccgccg cgcgtgccga tgaaagctgt tggtaagccg
     3301 ggcctcgata gcatcgaacc aacaccggct gtcaagaccg ggaagcggag acgagatgaa
     3361 ccactggcac cgagcgaggg tgtgcgtggc cctggcggcg tgcctcgcgg cgggggtcgc
     3421 gcggggcaac gagcgggtcg tcctgatggt gggcggcatc gacaagcaga tctacctgcc
     3481 ggcccgcctg gcccagcgcc tggactactt cgccgccgag ggcgtcgacg tggagctgcg
     3541 cagcgaggag tcgggcgtcg gcgcgcgcga ccagctgctg ctcggcacgg tcgacggggt
     3601 catcggcttc tacgatcaca cgatcgacct gcaggccaag ggcaagctgg tggagtcggt
     3661 cgtgcagttc gcgcgcgcgc cgggcgaggc gctgctggtc tccgggcgca gcgccgagcg
     3721 cgtgcgcacg ctggccgacc tgcgcggccg ccccgtcggc gtgaccggcc tgggct
//
LOCUS       DASVLN010000423         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_182975, whole genome shotgun sequence.
ACCESSION   DASVLN010000423 DASVLN010000000
VERSION     DASVLN010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182975"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VF453_10475"
     CDS             complement(<1..1107)
                     /locus_tag="VF453_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HEX7688125.1"
                     /translation="MFGNLKVGTRLALGFGAVVALLLVIAITSVLRLATVHESAVVIT
                     DQLYPKVALGSGIIRNALENGRYIRNMLLATNDGELAKNWQSLQANKLEIDGQLKKLG
                     GMVTSDKGKDEMRTVIERAQAADDKLERLHELAATDRAKGVEFMRAEFSPANEAFRTA
                     LDTLQQHQSQEMDDASRHGGEVYTTTSELVIGLSVGAVLLAAGIGFLITRNLGKVLGG
                     EPAYAAEVMRQVATGDFTVDVAVRAGDTSSLLFTTRQMVQSAGASVADVVRVMGAMAA
                     GDLTQKIEKSYTGSFGEMKDYVNETVGKLAQVVTEVNSSAEALAGASEEVSATAQSLS
                     QAASEQAAGVEETSASMEQMTASISQNTENAKVTD"
     gene            complement(1145..1663)
                     /locus_tag="VF453_10480"
     CDS             complement(1145..1663)
                     /locus_tag="VF453_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7688126.1"
                     /translation="MNAKTSASTIEPTQYLTFMLAQEQFAIGILGIKEIIEYHGVTEV
                     PMMPPCVRGVINLRGAVVPVIDLNARFGRKSSEVTKRTCVVIVESGLGTADRQVMGLV
                     VDAVNEVLDIAAADIEPPPAFGARVRSDFIQGVGKVKGRFVLLLDVDRALSIDEIAGI
                     GESVEAAEAVEA"
BASE COUNT          241 a          589 c          589 g          311 t
ORIGIN      
        1 gtcggtgacc ttggcgttct cggtgttctg ggagatcgag gccgtcatct gctccatcga
       61 ggcgctggtc tcctcgacgc cggcggcctg ctcgctggcg gcctgcgaca gcgactgggc
      121 cgtcgcgctc acctcttcgg atgcgcccgc cagcgcctcg gcgctcgagt tcacctcggt
      181 gacgacctgc gcgagcttgc cgacggtctc gttgacgtag tccttcatct cgccgaaaga
      241 gccggtgtag gacttctcga tcttctgcgt caggtcgccg gcggccatcg cgcccatcac
      301 gcgcaccacg tcggcgacgc tcgcgccggc cgactgcacc atctggcgcg tggtgaacag
      361 caggctgctc gtgtcgcccg cgcgcaccgc gacgtcgacg gtgaagtcgc ccgtcgcgac
      421 ctggcgcatg acctcggccg cgtaggcggg ctcgcccccc aggaccttgc ccaggttgcg
      481 cgtgatcagg aagccgatgc cggcagccag cagcaccgcg ccgaccgaca ggccgatcac
      541 gagctcgctc gtcgtggtgt agacctcgcc gccgtggcgg ctcgcgtcgt ccatctcttg
      601 cgactggtgc tgctgcagcg tatccagcgc ggtgcggaag gcctcgttgg cgggcgaaaa
      661 ctcggcgcgc atgaactcca cgcccttggc cctgtcggtg gccgcgagct cgtgcagtcg
      721 ctcgagcttg tcgtccgcgg cctgcgcgcg ctcgatcacc gtgcgcatct cgtccttgcc
      781 cttgtcgctg gtgaccatgc cgcccagctt cttgagctgg ccgtcgatct cgagcttgtt
      841 ggcctgcagc gactgccagt tcttcgccag ctcgccgtcg ttggtggcca gcagcatgtt
      901 gcggatgtag cggccgttct cgagcgcatt gcggatgatg ccgctgccga gcgcgacctt
      961 cggatagagc tggtcggtga tgaccaccgc ggattcgtgc accgtcgcga gccgcaggac
     1021 gctggtgatg gcgatgacga gcagcagcgc gacgacggcg ccgaagccca gagcgaggcg
     1081 ggtgccgacc ttgaggttgc cgaacatgtg gatcttccct ggatgacgaa acggggtggc
     1141 ccggtcaggc ctcgacggct tccgccgctt cgacggattc gccgatgccg gcgatctcgt
     1201 cgatggacag cgcgcggtcg acgtccagca gcagcacgaa gcggcccttg accttgccca
     1261 cgccctggat gaagtcgctg cgcacgcgcg cgccgaaggc cggcggcggc tcgatgtcgg
     1321 cggcggcgat gtcgagcacc tcgttgacgg cgtcgaccac caggcccatc acctggcggt
     1381 cggcggtgcc gaggccggac tcgacgatca ccacgcaggt gcgcttggtg acctcgctgg
     1441 acttgcggcc gaagcgcgcg ttcaggtcga tcaccggcac gacggcgccg cggaggttga
     1501 tcacgccgcg cacgcacgga ggcatcatcg gcacctcggt cacgccgtgg tactcgatga
     1561 tctccttgat gcccaggatg ccgatggcga actgctcctg ggccagcatg aaggtgaggt
     1621 actgcgtcgg ctcgatcgtc gacgcggagg tcttggcatt catcggcggg ttccttcccg
     1681 aaattgctca gaagcgcgcg aactgggatt catcgatcgg ctcgtcgccg
//
LOCUS       DASVLN010000424         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_506353, whole genome shotgun sequence.
ACCESSION   DASVLN010000424 DASVLN010000000
VERSION     DASVLN010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506353"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VF453_10485"
     CDS             complement(<1..545)
                     /locus_tag="VF453_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX7688127.1"
                     /translation="MSGLARFWRSLRGRLLLSLLAVLVLAAAAMGGSVYWNVLRQTEA
                     LFDYQLRQMALSLRDQGEIAPEDARALADGQLDYVVQIWREDGREVYATRPPVDPPGR
                     VVIGYADIVSGSMVWRTFGVVAQDRVIQVAQPARVRHDRAVQAALRSVVPLLGVAPAL
                     ALVIGWLVTRSLAPLQRLAGEV"
     gene            complement(686..1369)
                     /locus_tag="VF453_10490"
     CDS             complement(686..1369)
                     /locus_tag="VF453_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7688128.1"
                     /translation="MRLLLVEDDRMIGESLRAALKLEGHAVDWVTDAPAASGTLATER
                     FDLVLLDLGLPKGNGLDVLRGLRTRGDSTPVIVLTARDGVGDRVAGLDAGADDYLAKP
                     FELDELLARMRAVLRRHSGRATSRLVHEDLSFDPATREVTLRGEPVMLSARELAVLEA
                     LMQRPGAILSRAQLEDRLYGWGEEVESNAVSVYVHQLRKKLGPELIRTMRGVGYYLGR
                     RGAQAEGGE"
     gene            complement(1428..>2003)
                     /locus_tag="VF453_10495"
     CDS             complement(1428..>2003)
                     /locus_tag="VF453_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7688129.1"
                     /translation="PYTLKLAFTPDWGLRVGGDAWVRQVGGGGPAASHGGGDTTVVLK
                     RRFAVSDATAWGLELGEKLPTAGPVLGSGKADTTVNAIFSSDFAPGWHTDLNLNETRL
                     GVPSGQPRAWQTGWAAAFSRALSDTVGVTGELSGTRQAGAPSTAQFLAAASWNASNAA
                     VFDCGYARGLNDAAPRNQWFAGMTVRLGRLF"
BASE COUNT          258 a          767 c          729 g          249 t
ORIGIN      
        1 acctcgcccg cgagccgctg caacggcgcg agcgagcgcg tcacgagcca gccgatcacc
       61 agcgccagcg cgggcgcgac ccccagcagc ggcacgacgc tgcgcagcgc cgcctgaacc
      121 gcccggtcgt ggcgcacgcg cgcgggctgc gcgacctgga tcacgcggtc ctgcgccacg
      181 acgccgaagg tgcgccagac catgctgccg gagacgatgt ccgcgtagcc gatcaccacg
      241 cgtcccggcg ggtcgaccgg cggacgcgtc gcgtagacct cgcggccgtc ctcgcgccag
      301 atctgcacca cgtagtcgag ctggccgtcg gcgagcgcgc gggcgtcctc gggcgcgatc
      361 tcgccctggt cgcgcagcga cagcgccatc tggcgcagct ggtagtcgaa cagcgcctcc
      421 gtctggcgca gcacgttcca gtagaccgag ccgcccatcg cggcggccgc cagcacgagc
      481 accgccagca gcgacagcag cagccgcccg cgcagcgagc gccagaagcg cgcgaggccg
      541 ctcaccgggg cgcctccggc gcgcggatcc cggttcgagc cctcttgcag gcagccgggc
      601 ggtggctcac gaggcaagct ccgtcctggg gactgcggtg cgagcgcctt cgggcggccg
      661 ggcggcgctc atgccgtgcg ccccctcact cgccgccctc cgcctgagcg ccgcgtcggc
      721 ccaggtagta gcccacgccg cgcatcgtgc ggatcagctc cgggcccagc ttcttgcgca
      781 gctggtgcac gtagacgctg accgcgttgc tctcgacctc ctcgccccag ccgtagagcc
      841 gatcctccag ctgcgcgcgc gacaggatcg cgccggggcg ctgcatcagc gcctccagca
      901 cggcgagctc gcgcgccgag agcatcaccg gttcgccgcg cagcgttacc tcgcgcgtgg
      961 ccgggtcgaa gctcaggtcc tcgtgcacga ggcggctggt cgcgcggccg ctgtggcgtc
     1021 gcaggacggc gcgcatgcgg gcgagcagct cgtcgagctc gaagggcttg gcgaggtagt
     1081 cgtccgcgcc ggcgtccagg ccggcgacgc ggtcgcccac gccgtcgcgc gcggtcagca
     1141 cgatcaccgg cgtcgagtcc ccgcgcgtcc gcagcccgcg cagcacgtcg agcccgttgc
     1201 ccttgggcag gccgaggtcc agcagcacga ggtcgaaacg ctcggtcgcc agcgtgcccg
     1261 acgcggccgg ggcgtccgtc acccagtcga cggcgtggcc ttcgagcttg agcgccgcgc
     1321 gcaggctctc gccgatcatc cggtcgtctt ccacgagcag cagtcgcatc cggcgattgt
     1381 gcccgcccgc gaaggcgcgg cccgtcggac gagcgcccgc cgagctttca gaacaggcgc
     1441 ccgagccgca cggtcatccc ggcgaaccac tggttgcgcg gcgcggcgtc gttcaggccg
     1501 cgggcatagc cgcagtcgaa gacggcggcg ttcgaggcat tccagctcgc ggccgcgagg
     1561 aactgcgcgg tgctcggcgc gccggcctgg cgggtgcccg agagctcgcc cgtcacgccg
     1621 acggtgtcgc tgagcgcgcg cgagaacgcg gccgcccagc ccgtctgcca cgcgcgcggt
     1681 tgcccggagg gcacgcccag gcgcgtctcg ttcaggttca ggtcggtgtg ccagccgggc
     1741 gcgaagtcgc tgctgaatat cgcgttgacc gtggtgtcgg ccttgccgct gcccagcacc
     1801 gggccggcgg tcggcagttt ctcgccgagc tcgagtcccc aggccgtggc gtcgctgacc
     1861 gcgaagcggc gcttgaggac gaccgtggtg tcgccgccgc cgtgcgacgc ggcagggccg
     1921 ccgccgccga cctggcgcac ccaggcatcg ccgccgacgc gcaggcccca gtccggcgtg
     1981 aacgcgagct tgagggtgta ggg
//
LOCUS       DASVLN010000425         3293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_75284, whole genome shotgun sequence.
ACCESSION   DASVLN010000425 DASVLN010000000
VERSION     DASVLN010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75284"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..569)
                     /locus_tag="VF453_10500"
     CDS             complement(177..569)
                     /locus_tag="VF453_10500"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX7688130.1"
                     /translation="MADREDTTPGAPGGDRAPGDAPDFRAGLLKNLAGLALSAILTLA
                     SFWSAGTPSVWGPGVPVLLAALAIGQMGVHLVFFLHVSSGPEGTNNILALAFGVFVVG
                     LVVFGSIIIMDSLDLGMGAMDAMTRMQR"
     gene            complement(569..1207)
                     /locus_tag="VF453_10505"
     CDS             complement(569..1207)
                     /locus_tag="VF453_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7688131.1"
                     /translation="MRTAAIARAGATAPVEPEQVPVSEQGPSSRRVIVGYGFWLFLLS
                     DIILFSTFFAAYAVLAQRNAGGPVGTQLFHRDRVLLETACLLASSFTCGIFSLAVDVR
                     RRGLMLVFALLTFALGAVFLGIELAEFADMIGQGAGPGRSAFLSAFFALVGLHGLHVT
                     IGLAWLASMVAQVYTLGWRPFVLGRLVCFALFWHALDIVWIGVFTIVYLGAR"
     gene            complement(1204..3210)
                     /gene="cyoB"
                     /locus_tag="VF453_10510"
     CDS             complement(1204..3210)
                     /gene="cyoB"
                     /locus_tag="VF453_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEX7688132.1"
                     /translation="MLGKLTWNSIPWDQPIPLVAFGVVLLGILALLALATAKRWWPVI
                     WRDWICSVDHKRIGVMYVLLALVMLVRGFADALMMRAQQAMAAGGAQGYLPPEHFDQI
                     FSAHGTIMIFFMAMTFMIGLMNFVVPLQIGARDVAFPTLNSVSFWLSASGVLLTNLSL
                     VIGEFARTGWLAYPPLSELAYSPDVGVDYYLWSVQISGVGTLLAGINLVTTILKMRAP
                     GMGYGRMPIFVWTALASNLLIIAAFPILTGTLAMLQLDRYLGFHFFTNEAGGNAMMYV
                     NLIWAWGHPEVYILVLPAFGIYSEVVPAFSGRPLFGYRSMAAATLGICTISFMVWLHH
                     FFTMGAGADVNAFFGVATMIIAIPTGVKIFNWLFTLYGGTLRFAPPLMWAIGFMTSFV
                     IGGATGVLLAIPSVDFQLHNSLFLVAHFHNVIIPGVLFGAYAGYTYWFPKAFGFTLHE
                     GLGKAIFWCWYVGFFLAFMPLYVLGLGGATRRMQHYPELGWQPWFVVAAIGAGVILVG
                     IVLTVVQLVVSVRRRERLRDMTGDPWDGRTLEWALPSPPPPWNWRALPQVGGVDPFWT
                     AKREDGGRIPASADDGRHGLHLPRPSPVGFFVALFTSVGGFALVWHIWWLAILGLVGV
                     TATGLWRAWQVEHEFEVPAGELDAFDERAGTRLVPAASLQESAA"
BASE COUNT          595 a         1144 c         1177 g          377 t
ORIGIN      
        1 cgcggtggcc gtctccacgg agtcgcccgc gacggagccg cccgcgacgg cgtgcgcggc
       61 gagcaggcac agcacggccg ccgcggccgc gcgcttgtcg ttgatcctca tcggctccct
      121 cgcgatggac gcgggatcgt cgcccgcgat gcgcacggag gttatcaccg ccgcgctcac
      181 cgctgcatgc gggtcatcgc gtccatcgcg cccatgccga ggtcgaggct gtccatgatg
      241 atgatggagc cgaacaccac cagccccacg acgaagacgc cgaacgccag cgcgaggatg
      301 ttgttggtgc cctccgggcc gctggacacg tgcaggaaga acaccaggtg cacgcccatc
      361 tgcccgatcg cgagcgccgc gagcagcacc ggaacgccgg gcccccagac gctgggcgtg
      421 cccgccgacc agaacgaggc gagcgtgagg atcgccgaca gcgcgagccc ggcgaggttc
      481 ttcagcaggc ccgcgcggaa gtcgggcgcg tcgcccgggg cgcggtcgcc gccgggcgcg
      541 ccgggggtgg tgtcttcgcg gtcggccatc agcgcgctcc caggtagacg atggtgaaca
      601 cgccgatcca gacgatgtcc agcgcgtgcc agaacagggc gaagcagacc agccgcccga
      661 gcacgaacgg ccgccatccc agcgtgtaga cctgcgcgac catcgacgcg agccaggcca
      721 ggccgatcgt cacgtgcagg ccgtgcaggc ccaccagcgc gaagaacgcg gagaggaacg
      781 cgctgcggcc cgggccggcg ccctgcccga tcatgtcggc gaactcggcc agctcgatgc
      841 cgaggaagac ggcgccgagc gcgaaggtca gcagcgcgaa cacgagcatc agcccgcgcc
      901 ggcgcacgtc gaccgccagc gagaagatgc cgcaggtgaa gctcgacgcg agcaggcagg
      961 ccgtctccag cagcacgcgg tcgcggtgga acagctgcgt gccgaccggc ccgcctgcat
     1021 tgcgctgcgc cagcaccgcg taggccgcga agaaggtcga gaacaggatg atgtcgctca
     1081 gcaggaacag ccagaagccg tagccgacga tcacgcgccg cgacgacggg ccctgctccg
     1141 atacgggtac ctgttccggt tcgaccggcg cggtggcgcc ggcacgcgcg atcgcggcgg
     1201 tcctcatgcg gcggactcct gcagcgacgc ggccggcacg agtcgcgtgc cggcgcgctc
     1261 gtcgaaggcg tcgagctcgc cggcaggcac ctcgaactcg tgctcgacct gccaggcgcg
     1321 ccacaggccg gtcgccgtca cgccgacgag cccgaggatc gccagccacc agatgtgcca
     1381 tacgagcgcg aagccgccga cgctcgtgaa cagcgcgacg aagaagccca ccgggctcgg
     1441 gcgcggcagg tgcaggccgt ggcggccgtc gtccgcggac gcggggatgc ggccgccgtc
     1501 ctcgcgcttg gcggtccaga acgggtcgac gccgccgacc tgcggcagcg cgcgccagtt
     1561 ccacggcggc ggcggcgagg gcagcgccca ctcgagcgtg cggccgtccc acgggtcgcc
     1621 cgtcatgtcg cgcaggcgct cgcgccgccg cacgctcacg accagctgca cgacggtcag
     1681 cacgatgccc acgaggatca cccccgcgcc gatcgcggcg accacgaacc acggctgcca
     1741 gccgagctcg ggatagtgct gcatgcgccg cgtcgcgccg cccaggccga gcacgtacag
     1801 cggcatgaac gcgaggaaga agccgacgta ccagcaccag aagatcgcct tgcccagccc
     1861 ctcgtgcagc gtgaagccga aggccttcgg gaaccagtac gtgtagccgg cgtaggcgcc
     1921 gaacagcacg cccgggatga tgacgttgtg gaagtgcgcg accaggaaca ggctgttgtg
     1981 cagctggaag tcgaccgacg ggatcgccag cagcacgccc gtggcgccgc cgatcacgaa
     2041 gctcgtcatg aagccgatcg cccacatcag cggcggcgcg aagcgcagcg tgccgccgta
     2101 cagcgtgaac agccagttga agatcttcac gccggtcggg atcgcgatga tcatcgtcgc
     2161 gacgccgaag aaggcgttga cgtccgcgcc cgcgcccatc gtgaagaagt ggtgcagcca
     2221 caccatgaac gagatggtgc agatgcccag cgtggccgcg gccatcgagc ggtagccgaa
     2281 cagcggccgc ccggagaacg ccggcaccac ctccgagtag atgccgaacg cgggcagcac
     2341 gaggatgtag acctcggggt ggccccaggc ccagatgagg ttgacgtaca tcatcgcgtt
     2401 gccgccggcc tcgttggtga agaagtggaa gcccaggtag cggtccagct gcagcatcgc
     2461 cagcgtgccg gtgaggatgg gaaaggccgc gatgatgaga aggttcgacg cgagcgccgt
     2521 ccacacgaag atcggcatgc ggccgtagcc catgccgggc gcgcgcatct tgaggatggt
     2581 ggtgacgagg ttgatgcccg ccagcagcgt ccccacgccc gagatctgca ccgaccacag
     2641 gtagtagtcg acgcccacgt ccggcgagta ggcgagctcg gacagcggcg ggtaggcgag
     2701 ccagccggtg cgcgcgaact cgccgatcac gagcgacagg ttggtcagca gcacgcccga
     2761 ggcggacagc cagaagctca ccgagttcag cgtcgggaac gcgacgtcgc gcgcgccgat
     2821 ctgcagcggg acgacgaagt tcatgaggcc gatcatgaag gtcatcgcca tgaagaagat
     2881 catgatcgtc ccgtgcgccg agaagatctg gtcgaagtgc tccggcggca ggtagccctg
     2941 cgcgccgccg gcggccatcg cctgctgcgc gcgcatcatc agcgcgtcgg cgaagccgcg
     3001 caccagcatc acgagcgcca gcagcacgta catcacgccg atgcgcttgt ggtcgacgct
     3061 gcagatccag tcgcgccaga tgacgggcca ccagcgcttg gcggtggcga gcgcgagcag
     3121 cgcgaggatg cccagcagca cgacgccgaa ggcgacgagc gggatcggct ggtcccacgg
     3181 gatcgagttc caggtgagct tgcccagcat gtcagcgcat cgcctccatc agggacgccg
     3241 gcgcggccgc cggcgcggtg cggggagcgc ggcaggccgc cgcggcctgg gcc
//
LOCUS       DASVLN010000426         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_452439, whole genome shotgun sequence.
ACCESSION   DASVLN010000426 DASVLN010000000
VERSION     DASVLN010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452439"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..285)
                     /locus_tag="VF453_10515"
     CDS             complement(73..285)
                     /locus_tag="VF453_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX7688133.1"
                     /translation="MSSTPTSTPVEVTAADLQGPGVVFCPNPKMPLWSAHPKVYIDLS
                     HEGRGRCPYCGTTYQLAAGVKIAAGH"
     gene            complement(313..1236)
                     /locus_tag="VF453_10520"
     CDS             complement(313..1236)
                     /locus_tag="VF453_10520"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX7688134.1"
                     /translation="MSMDDRDGKIWMDGELVDWRDAKIHVLTHTLHYGCGAFEGVRAY
                     KTAQGTAIFRLREHTERLFNSAKILRMKIPFTPEQVMDAQRAVVRENKLESCYLRPLT
                     WIGSEKLGVSPKGNRIHLMVAAWAWGAYLGEEGLKRGIRVKTSSYTRHHVNITMTQAK
                     AVSNYSNSILANMEATEDGYDEALLLDAAGFVSEGAGENIFVIKNGVVYTPDLSAGAL
                     NGITRNTIFAICQDLGLKLVEKRITRDEIYIADEAFFTGTAAEVTPIRELDRIELGAG
                     SRGPITEKIQAAFFDIVNGRNPKYAEWLTAV"
     gene            1358..>1602
                     /locus_tag="VF453_10525"
     CDS             1358..>1602
                     /locus_tag="VF453_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688135.1"
                     /translation="MPTPNVQLDHAAHRPAATRRALRLLAVDVGIGANVGTLFRIADA
                     LGVEHLHLAGDSPVPPDPRIRRSARSAERFVPWSHAA"
BASE COUNT          219 a          537 c          575 g          271 t
ORIGIN      
        1 cgcgcgcacg gcgcagggcc ggcgcgtcgt cgcgcggcgc gcaggcaggc atgccgggcg
       61 cgatctccgg gatcaatgcc cggcggcgat cttcacgccg gcggcgagct ggtacgtcgt
      121 gccgcagtac gggcagcggc cgcgtccttc gtggctaagg tcgatgtaga ccttcgggtg
      181 cgcgctccac agcggcatct tcgggttcgg gcagaagacg acgccggggc cttgcaggtc
      241 ggcggcggtg acttcgacgg gcgtggacgt gggagtgctg gacatggacg tgctcgggat
      301 gggatcggat gctcagaccg ccgtcaacca ctcggcgtac ttcggattgc ggccgttgac
      361 gatgtcgaag aacgccgcct ggatcttctc ggtgatcggg ccgcgcgagc ccgcgcccag
      421 ctcgatgcga tcgagctcgc ggatcggcgt cacctcggcg gcggtaccgg tgaagaaggc
      481 ctcgtcggcg atgtagatct cgtcgcgcgt gatgcgcttc tcgaccagct tcaggcccag
      541 gtcctgacag atcgcgaaga tcgtgttgcg cgtgatgccg ttgagcgcgc cggccgacag
      601 gtcgggcgtg tagacgacgc cgttcttgat gacgaagatg ttctcgcccg cgccctcgga
      661 cacgaagccg gcggcgtcca gcagcagcgc ctcgtcgtag ccgtcctcgg tcgcctccat
      721 gttggcgagg atcgagttcg agtagttcga caccgccttg gcctgcgtca tcgtgatgtt
      781 gacgtggtgg cgcgtgtagc tggaggtctt gacgcggatg ccgcgcttga ggccttcctc
      841 gccgaggtag gcgccccagg cccaggccgc gaccatcagg tggatgcggt tgcccttggg
      901 gctgacgccc agcttctcgg agccgatcca ggtcagcggg cgcaggtagc agctctcgag
      961 cttgttctcg cgcacgaccg cgcgctgcgc gtccatcacc tgctcgggcg tgaacgggat
     1021 cttcatgcgc aggatcttgg ccgagttgaa caggcgctcg gtgtgctcgc gcaggcggaa
     1081 gatcgccgtg ccctgggcgg tcttgtacgc gcgcacgccc tcgaaggcgc cgcagccgta
     1141 gtgcagcgtg tgggtgagga cgtggatctt cgcatcgcgc cagtcgacga gctcgccgtc
     1201 catccagatc ttgccgtcgc ggtcgtccat ggacatgtga tgttcctcgt cgaagaaggt
     1261 ggaatcctgg ggaaaccccg attctacgga acgccggcgc acgaggcgcg cgctgcggcg
     1321 ccgcggcggc cgagcgtcgt gcagaattcc gccgcccatg ccgactccca acgtccagct
     1381 cgaccacgcc gcccaccggc ccgccgcgac ccgccgggcg ctgcggctgc tggccgtgga
     1441 cgtcgggatc ggcgccaacg tcggcacgct gttccggatc gccgacgcgc tcggcgtcga
     1501 gcacctgcac ctcgcgggcg actcgccggt gccgcccgat ccgcgcatcc gccgcagcgc
     1561 gcgctcggcc gagcgcttcg tgccgtggtc gcacgccgcc ga
//
LOCUS       DASVLN010000427         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_452470, whole genome shotgun sequence.
ACCESSION   DASVLN010000427 DASVLN010000000
VERSION     DASVLN010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452470"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..901)
                     /locus_tag="VF453_10530"
     CDS             complement(110..901)
                     /locus_tag="VF453_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7688136.1"
                     /translation="MKHDAAAPPPWRRALAPVLAVLVFLAAWEGGVRAFHVPPYLLPA
                     PSLIAATLADNFGSLMASWWFTVRITFAALALAIAGGVAIAAVFALSRTAERALAPFA
                     VVLQVTPIVAIAPLIMIVTDSPLATVLVCAWIVAFFPILANTLTGLRSADERLRDFLR
                     LHRATRWQTLRHLLVPAALPSFMTGLRIAGGLALIGEVVAEFVAGVAGRDTGLASRIL
                     EADFRTEIPKMFAALSLVALTGVAIYLLTAALSRALLGRWHESER"
     gene            complement(978..>1641)
                     /locus_tag="VF453_10535"
     CDS             complement(978..>1641)
                     /locus_tag="VF453_10535"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7688137.1"
                     /translation="SLLGASGCGKSTVLRLMAGLDAPTTGRVERADAGEIPGVVFQDA
                     ALMPWATVRRNVELPLRLRGEPREAMRARVDAALAAVGLADSAEAVPRELSGGMRMRA
                     SLARALVTRPRLWLLDEPFGALDEITRFALNQTLLALCQPGDGTPPATAVFVTHSVYE
                     AVFLSRRIVVMARPGRVVAEIAVDEPYPRGPAFRTTPRFTAWCAEVSDALQRASATEA
                     AA"
BASE COUNT          230 a          573 c          663 g          175 t
ORIGIN      
        1 cgcgcgcacg ctcgcgcggg cgcggcgggc ggcggtgcat tgaggcgcgg cggggtgaat
       61 cccaccggct gcggcgcgtc gtcgtgcggg agggcggatg cggttggcgt cagcgctcgc
      121 tctcgtgcca ccgccccagc aacgcccggc tgagcgccgc ggtcagcagg tagatcgcca
      181 cgcccgtgag cgcgacgagg ctcagcgccg cgaacatctt ggggatctcg gtgcggaagt
      241 cggcttcgag gatgcgcgag gcgaggcccg tgtcgcggcc ggcgacgccg gcgacgaact
      301 cggcgaccac ctcgccgatc agcgcgaggc cgcccgcgat gcgcaggccg gtcatgaacg
      361 agggcagggc ggcgggcacc agcaggtggc gcagcgtctg ccagcgcgtc gcacggtgca
      421 ggcgcaggaa gtcgcgcagt cgctcgtcgg cgctgcgcag gccggtgagc gtgttggcga
      481 ggatggggaa gaacgcgacg atccacgcgc agacgagcac ggtggcgagc gggctgtcgg
      541 tgacgatcat gatcagcggc gcgatcgcga cgatgggcgt cacctgaagc accaccgcga
      601 acggcgccag cgcgcgctcg gcggtgcgcg acagcgcgaa gacggcggcg atcgcgacgc
      661 cgcccgcgat cgcgagcgcc agcgccgcga acgtgatgcg cacggtgaac caccagctcg
      721 ccatcagcga gccgaagttg tcggccagcg tcgccgcgat cagcgacggc gcgggcagca
      781 ggtagggcgg gacgtggaac gcgcgcacgc cgccttccca cgcggcgagg aacacgagca
      841 ccgccagcac cggcgcgaga gcgcggcgcc acggcggcgg agcggcggcg tcgtgcttca
      901 tgcggcggcc gctccggcgg cctgcggcgt cgtgcgatgg cctggccatc gcggggcgtg
      961 cggcgacgat cgcggcgtca tgccgcggcc tccgtggcgc tggcgcgctg cagcgcgtcc
     1021 gagacctcgg cgcaccacgc cgtgaagcgg ggcgtcgtcc ggaaggcggg tccgcgcggg
     1081 tagggttcgt cgacggcgat ctcggcgacc acgcgtccgg gccgcgccat caccacgatg
     1141 cgccggctca ggaagacggc ctcgtagacg ctgtgcgtga cgaagacggc cgtggcgggc
     1201 ggcgtgccgt cgccgggctg gcacagcgcg agcaaggtct ggttgagggc gaagcgcgtg
     1261 atctcgtcga gcgcgccgaa gggctcgtcc agcagccaaa ggcgcggacg cgtgacgagc
     1321 gcgcgcgcga gcgacgcccg catgcgcatg ccgcccgaga gctcgcgcgg cacggcctcg
     1381 gcggagtcgg ccagtcccac cgcggcgagc gccgcgtcga ccctggcgcg catcgcctcg
     1441 cgcggttcgc cgcgcaggcg cagcggcagc tcgacgttgc ggcgcaccgt ggcccagggc
     1501 atcagcgcgg cgtcctggaa gacgacgccg gggatctcgc cggcgtccgc gcgctcgacg
     1561 cggccggtcg tcggggcgtc gaggccggcc atgagccgca gcaccgtgct cttgccgcag
     1621 cccgaggcac cgagcaggct g
//
LOCUS       DASVLN010000428         5465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_235835, whole genome shotgun sequence.
ACCESSION   DASVLN010000428 DASVLN010000000
VERSION     DASVLN010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235835"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF453_10540"
     CDS             complement(<1..197)
                     /locus_tag="VF453_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HEX7688138.1"
                     /translation="MSLPFVLLALAIAAAWLPPLRVARRAFPTWMALFAAAVAAGVVD
                     GVLRAPAVASLGMLCALAWMSR"
     gene            complement(221..1234)
                     /locus_tag="VF453_10545"
     CDS             complement(221..1234)
                     /locus_tag="VF453_10545"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX7688139.1"
                     /translation="MGKKVSLTQYLVEQQREHGRIPSQLRLLIEVVARACKHIAIAVN
                     KGALGDVMGSADTENVQGEIQKKLDIISNEVLIEANEWGGHLAAMASEEMDTIHVVPN
                     RYPQGEYLLLFDPLDGSSNIDVNVSIGTIFSVLRKVGHHHGVEEGDFLQAGRNQAAAG
                     YCIYGPQTMLVLTVGDGVAMFTLDREMGSWVLTQENVRIPEDTKEFAINMSNQRHWAE
                     PVSRYIDECLLGKDGPRGKDFNMRWVASMVADVHRILSRGGVFMYPWDKREPEKAGKL
                     RLLYEAAPMAFLVEQAGGAATNGRQRILDLVPGKLHERVAVFLGSRNEVERATQYHLD
                     ANA"
     gene            complement(1381..4080)
                     /gene="pepN"
                     /locus_tag="VF453_10550"
     CDS             complement(1381..4080)
                     /gene="pepN"
                     /locus_tag="VF453_10550"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX7688140.1"
                     /translation="MREGTPTVVRREDYLAPAYWVRTVDLTFDLDPAKTIVHSKLEIK
                     RNLERNTEPDAPTEPLRLDGEGLNLLRVLVDGQSVSFRHEDGQLVIDAPAADRFTLEI
                     RNTVCPEKNTELSGLYTSGGGFFTQCEAQGFRRITYFFDRPDVMAVYTVTLKADRKKY
                     PVLLSNGNLVEQGDLDNGRHFARWNDPFPKPSYLFALVGADLVAREQKIVSRGGKEHL
                     LQVYVRRGDLDKTEHAMNSLVASVVWDEARFGLPLDLERFMIVAVEDFNMGAMENKGL
                     NLFNTKFVLASPATATDTDYALIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFR
                     DQEFSQDMAGSPSARAVKRIDDVRFLRQQQFPEDAGPMSHPVRPDSYVAIDNFYTTTV
                     YEKGSELVRMMQTLVGRAGFGKGITEYFRRHDGQAVTCDAFAQAIADTNPGSPLAAQL
                     DAFKRWYSQAGTPRLTARGAHDETTGAFTLTLSQDFSAPASVVPTTPRQPFVLPVRFA
                     LFTRDGAPVPLKLEGEADAPLERVLVLSDAEASYRFADVPAGAVPSVLRDFSAPVILD
                     DGLSDADRLVLLAHDTDPVNRWEAGQRLALQQLVAAVAGDGTLALEPAFVEAMRRVLR
                     DPALDPAFKELVLTPPSEAYVAEQLAVPVDPQRVHAVSQAMADQLADALAEDWAWAYE
                     QHQVRDGYRPDGEQSARRALANLALGMLVRRAARTGDTVWPGKAYQRFKDAGNMTDRL
                     GALAALVAAHSELAAPALARFHALFRHEPLVVDKWFALQARAPEQDGRVFANVKALLQ
                     HPDFSLSNPNRVRSLVLSLCNFNPAAFHRRDAKGYVFWAEKVCELDALNPQVASRLAR
                     ALDRWRQLAEPYRGAAREAIARVAAKPELSPDVREVVERALRD"
     gene            complement(4237..5338)
                     /gene="prfB"
                     /locus_tag="VF453_10555"
     CDS             complement(join(4237..5256,5258..5338))
                     /gene="prfB"
                     /locus_tag="VF453_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552084.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX7688141.1"
                     /translation="MDIERINAIGNTIADLSERTEALRGYLDYDRKSHRLTEVNATLE
                     NPAVWNEPQRAQELGREKRSLEQVVSTIDHLTTNLADNAELYEMVKEEGDVDSLQQIE
                     ADAAKLRETVETLEFRRMFSNPSDPLNCFLDIQSGAGGTEAQDWAQMLLRQYLRYCER
                     KGFKAEVMEETEGDVAGIKSATLKVEGEYAFGHLRTETGVHRLVRKSPFGAGLRQTSF
                     ASVFVYPEVDDTIEIEINPADVRTDTFRASGAGGQHINKTDSAVRLTHIPTGIVVQCQ
                     NDRSQHRNRDEAWQMLRSRLFEHEMRKQREEQQKLEDSKTDVGWGHQIRSYVLDQSRV
                     KDLRTNVEVSATQKVLDGDLDQFIAASLKQGL"
     gene            5280..>5465
                     /locus_tag="VF453_10560"
     CDS             5280..>5465
                     /locus_tag="VF453_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688142.1"
                     /translation="MRSLRSAMVLPMALMRSMSMVGRSVSRPSHGACAAVGVAQSVRF
                     SHGAGAHGRPGAGMLARM"
BASE COUNT          757 a         1823 c         1992 g          893 t
ORIGIN      
        1 cgcgacatcc acgcgagggc gcacagcatg cccagcgagg cgacggccgg cgcacgcagg
       61 acgccgtcga cgacgcccgc cgcgaccgcg gccgcgaaca gcgccatcca ggtgggaaag
      121 gcccgccgcg cgacgcgcag cggcggcagc caggccgcgg cgatggccag ggccagcagg
      181 acgaagggaa ggctcacggg cggatcccga ggggcctgcc tcaggcgttc gcgtccaggt
      241 ggtactgcgt cgcccgctcc acctcgttcc tcgacccgag gaacacggcc acgcgctcgt
      301 gcagcttgcc gggcaccagg tcgaggatgc gctggcggcc gttggtggcg gcgccgccgg
      361 cctgttcgac gaggaaggcc atcggcgcgg cctcgtagag caggcgcagc ttgccggcct
      421 tctcgggctc gcgcttgtcc caggggtaca tgaagacgcc gccgcggctc aggatgcggt
      481 ggacgtcggc gaccatcgag gcgacccagc gcatgttgaa gtccttgccg cgggggccgt
      541 ccttgcccag caggcactcg tcgatgtagc gcgacaccgg ctcggcccag tgccgctggt
      601 tggacatgtt gatcgcgaat tccttcgtgt cctcggggat gcgcacgttc tcctgcgtca
      661 gcacccacga gcccatctcg cgatcgagcg tgaacatcgc gacgccgtcg cccacggtca
      721 gcaccagcat ggtctgcggg ccgtagatgc agtagccggc ggccgcctgg ttcctgccgg
      781 cctgcaggaa gtcaccctcc tcgacgccgt ggtggtggcc gaccttgcgc agcaccgaga
      841 agatcgtgcc gatcgagacg ttgacgtcga tgttggacga gccgtcgagc gggtcgaaca
      901 gcagcaggta ctcgccctgc ggatagcggt tgggcacgac gtggatcgtg tccatttcct
      961 cggacgccat cgccgcgagg tggccgcccc attcgttggc ctcgatcagc acctcgttgg
     1021 agatgatgtc gagcttcttc tggatctcgc cctggacgtt ctcggtgtcg gccgagccca
     1081 tgacgtcgcc gagcgcgccc ttgttgaccg cgatcgcgat gtgcttgcac gcccgcgcga
     1141 cgacctcgat cagcagccgc agctgcgacg ggatgcggcc gtgctcgcgc tgctgctcga
     1201 cgaggtactg ggtgagactg accttcttgc ccatggtggt ggatgctctc gagagtggtg
     1261 gatccgatgg ttgcgcatgc ggtaccgggt atgcggaacc gcatgcgcaa cgaccggggt
     1321 cgccctggca agaacggaac cgggtcgacg acgcgacccg gttctcggct tcctggaagg
     1381 tcagtccctg agcgcgcgct cgacgacctc gcgcacgtcg gggctcagct ccggcttggc
     1441 ggcgacgcgg gcgatggcct cgcgcgcggc gccgcgatac ggctcggcga gctggcgcca
     1501 gcgatcgagc gcccgggcca ggcgcgaagc gacctgcggg ttcagcgcgt cgagctcgca
     1561 gaccttctcg gcccagaaca cgtagccctt cgcgtcccgg cgatggaacg cggccgggtt
     1621 gaagttgcac agcgagagca cgaggctgcg cacgcggttg gggttcgaca gcgagaagtc
     1681 cggatgctgc agcagcgcct tcacgttggc gaagacgcgg ccgtcctgct ccggcgcgcg
     1741 cgcctgcagc gcgaaccact tgtcgacgac cagcggctcg tggcggaaca gcgcatggaa
     1801 gcgtgcgagc gcgggcgccg cgagctccga gtgcgcggcc acgagggccg ccagtgcgcc
     1861 caggcgatcg gtcatgttgc cggcgtcctt gaagcgctgg taggccttgc cgggccacac
     1921 ggtgtcgccg gtgcgcgcgg cgcggcgcac gagcatgccc agcgccaggt tggcgagcgc
     1981 gcggcgggcg ctctgctcgc cgtccgggcg atagccgtcg cgcacctggt gctgctcgta
     2041 cgcccaggcc cagtcctcgg cgagcgcgtc ggcgagctgg tcggccatcg cctggctcac
     2101 cgcgtgcacg cgctgcgggt cgaccggcac cgcgagctgc tcggcgacgt aggcctcgga
     2161 cggcggcgtg agcaccagct ccttgaacgc cggatccagc gccgggtcgc gcagcacgcg
     2221 gcgcatcgcc tcgacgaacg cgggttcgag cgccagcgtg ccgtcgcccg cgaccgcggc
     2281 gaccagctgc tgcagcgcga gccgctggcc ggcctcccag cggttgaccg ggtccgtgtc
     2341 gtgggcgagc agcacgaggc gatcggcgtc ggacaggccg tcgtccagga tgaccggcgc
     2401 cgagaagtcg cgcagcaccg agggcaccgc gccggcgggc acgtcggcga agcggtacga
     2461 ggcctcggcg tcggacagca ccagcacgcg ctcgagcggc gcgtccgcct cgccctcgag
     2521 cttcagcggc acgggcgcac cgtcgcgcgt gaacagggcg aagcgcaccg gcagcacgaa
     2581 gggctggcgc ggcgtcgtgg gcacgacgct cgcgggcgcg gagaagtcct gcgacagcgt
     2641 gagcgtgaag gcgcccgtcg tctcgtcgtg cgcgccgcgc gcggtcagcc gcggcgtgcc
     2701 ggcctgcgag taccagcgct tgaaggcgtc gagctgcgcc gccagcgggc tgccggggtt
     2761 cgtatcggcg atcgcctgcg cgaacgcgtc gcaggtcacc gcctggccgt cgtggcggcg
     2821 gaagtattcg gtgatgccct tgccgaagcc cgcgcggccg accagcgtct gcatcatgcg
     2881 caccagctcc gagcccttct cgtagaccgt ggtcgtgtag aagttgtcga tcgcgacgta
     2941 gctgtccggg cgcaccgggt gcgacatcgg gccggcgtcc tcggggaact gctgctggcg
     3001 caggaagcgc acgtcgtcga tgcgcttgac cgcgcgcgcc gacggcgagc cggccatgtc
     3061 ctgcgagaac tcctggtcgc ggaacaccgt gaggccttcc ttgagcgaga gctggaacca
     3121 gtcgcggcag gtgacgcggt tgccggtcca gttgtggaag tactcgtggc cgacgacgct
     3181 ctcgatcagc gcgtagtcgg tgtcggtggc ggtcgccggg ctcgcaagga cgaacttggt
     3241 gttgaacagg ttcagcccct tgttctccat cgcgcccatg ttgaagtcct cgacggcgac
     3301 gatcatgaag cgctcgaggt ccagcggcag gccgaagcgc gcctcgtccc acaccaccga
     3361 cgcgacgagc gagttcatcg cgtgctcggt cttgtcgagg tcgccgcggc gcacgtagac
     3421 ctgcagcagg tgctccttgc cgccgcggct gacgattttc tgctcgcgcg cgaccaggtc
     3481 ggcgccgacc agggcgaaca ggtacgaggg cttcgggaac gggtcgttcc agcgcgcgaa
     3541 atggcggcca ttgtccaggt cgccctgctc gacgaggttg ccgttggaca gcagcaccgg
     3601 gtacttcttc cgatccgcct tcagcgtgac cgtgtagacg gccatcacgt cggggcggtc
     3661 gaagaagtag gtgatgcgac ggaagccctg cgcctcgcac tgcgtgaaga agccgccgcc
     3721 cgaggtgtac aggcccgaca gctcggtgtt cttctcgggg cagaccgtgt tgcggatctc
     3781 gagcgtgaag cgatccgccg ccggtgcgtc gatgaccagc tggccgtcct cgtggcggaa
     3841 cgagacgctc tggccgtcga ccagcacccg cagcaggttc aggccctcgc cgtccaggcg
     3901 cagcggctcg gtcggcgcgt cgggctccgt gttgcgctcc aggttccgct tgatctcgag
     3961 cttgctgtgg acgatggtct tggcggggtc gaggtcgaac gtcaggtcga cggtgcggac
     4021 ccagtaggcg ggcgcgaggt agtcctcgcg gcggacgacg gtgggggtgc cttcgcgcat
     4081 ggcaggttcc tcagtgacgg ggttgtttgg agcgatgcga agcgagccgg tcgccggccg
     4141 caggcccgtc cgagggactg gcacggaacc ggctttgccg ggccgtcgcc agggccccct
     4201 cggggggcag cgaccagcgg gagcgtgggg gtacgttcaa aggccttgct tcaggctcgc
     4261 ggcgatgaac tggtcgaggt cgccgtcgag caccttctgc gtggccgaga cctcgacgtt
     4321 ggtgcgcagg tccttcacgc ggctctggtc gagaacgtag gagcggatct ggtgacccca
     4381 gccgacgtcg gtcttggagt cctcgagctt ctgctgttcc tcgcgctgct tgcgcatctc
     4441 gtgctcgaac aggcgcgagc gcagcatctg ccaggcctcg tcgcggttgc ggtgctgcga
     4501 gcggtcgttc tggcactgca ccacgatgcc ggtcgggatg tgcgtcagcc gcaccgccga
     4561 gtcggtcttg ttgatgtgct ggccgccggc gccggacgcg cggaaggtgt cggtgcgcac
     4621 gtcggcgggg ttgatctcga tctcgatcgt gtcgtcgacc tcggggtaga cgaacaccga
     4681 tgcgaacgag gtctgccgca ggccggcgcc gaacgggctc ttgcgtacca ggcggtgcac
     4741 gccggtctcg gtgcgcaggt ggccgaacgc gtactcgccc tcgaccttca gcgtcgcgct
     4801 cttgatgccc gcgacgtcgc cttcggtctc ctccatcacc tcggccttga agcccttgcg
     4861 ctcgcagtag cgcaggtact ggcgcagcag catctgcgcc cagtcctggg cctcggtgcc
     4921 gccggcgccg gactggatgt cgaggaagca gttcagcgga tccgacgggt tgctgaacat
     4981 gcggcggaac tcgagcgtct cgaccgtctc ccgcagcttg gccgcgtcgg cctcgatctg
     5041 ctgcaggctg tcgacgtcgc cttcctcctt gaccatctcg tagagctcgg cgttgtcggc
     5101 caggttggtc gtcaggtggt cgatggtcga gaccacctgc tcgagcgagc gcttctcgcg
     5161 cccgagctcc tgggcccgct ggggctcgtt ccagaccgcc gggttctcca gcgtcgcgtt
     5221 gacttccgtc agccgatgcg acttgcggtc gtagtcaaag ataccccctg agcgcttcgg
     5281 tgcgctcgct caggtcggcg atggtgttgc cgatggcgtt gatgcgttcg atgtccatgg
     5341 tcgggcggtc cgtttcccgt ccgtcgcacg gcgcgtgcgc ggcggtgggt gttgctcaaa
     5401 gcgtgcgatt ttcgcatggc gcgggcgcgc acgggaggcc gggcgccggt atgctcgcgc
     5461 gcatg
//
LOCUS       DASVLN010000429         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_183192, whole genome shotgun sequence.
ACCESSION   DASVLN010000429 DASVLN010000000
VERSION     DASVLN010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183192"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VF453_10565"
     CDS             complement(<1..656)
                     /locus_tag="VF453_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7688143.1"
                     /translation="MAWLTPAQRATLLANDWFAELPEALRDEALESAVPRRLREGERL
                     YGKDDEGDAWYGIVEGAMRLGATAPDGRQGLLAILEPGTWFGDTSLLDGLPRPHDAVA
                     HAPTMLLAVARTPFQSLLDRYPMLYRHLLELHCRRERLMFLALEAWTAYPLDERLARH
                     LQHLARGHGRRVGEDIAIELHLPQEQLAQLLGVSRQRVSQLLRDWEQHGRLHFRYGRI
                     V"
     gene            952..1884
                     /locus_tag="VF453_10570"
     CDS             952..1884
                     /locus_tag="VF453_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEX7688144.1"
                     /translation="MRSTLAKWLAVAAIALSALPLPAWATGYAQTKYPIVLVHGLFGF
                     DNIGPLEYFYGIPEDLRANGATVYVAQVSAANSTEVRGEQLLTYVKQVLAVTGAKKVN
                     LIGHSHGGPTVRYVASVAPQLVASVTSVAGPNQGAALADALNGTLPAGSISRTVVAGA
                     VNAFAAIIELVSGDAGAPQNSSAALNSLSSAGMAKFNASHPEGLPTSSCGQGPSTVNG
                     VRYWSWSGAQPYTNVLDPIDPSLALTSLVYGGAKNDGLVGSCSSHLGTVLRDDYAMNH
                     LDEVNQTAGLVNLFETNPKTVFRDHANRLKNAGL"
BASE COUNT          300 a          736 c          677 g          277 t
ORIGIN      
        1 acgatgcggc cgtagcggaa gtgcagccgg ccgtgctgct cccagtcgcg cagcagctgg
       61 ctcacgcgct ggcgcgagac gcccagcagc tgcgcaagct gctcctgcgg caggtgcagc
      121 tcgatcgcga tgtcctcgcc cacgcgccgg ccatggcccc gggcgaggtg ctggaggtgg
      181 cgcgccaggc gctcgtcgag cggataggcg gtccaggcct ccagcgcgag gaacatcagg
      241 cgctcgcgcc ggcagtgcag ctcgaggaga tggcgataca gcatggggta tcggtcgagc
      301 agggactgga acggcgtccg cgcgaccgcg agcagcatcg tcggcgcatg cgcgaccgcg
      361 tcgtgcgggc gcggcaggcc gtccagcagc gaggtgtcgc cgaaccacgt gcccggctcg
      421 aggatcgcca gcaggccctg gcggccgtcg ggcgcggtcg cgcccaggcg catcgcgccc
      481 tcgacgatgc cgtaccacgc gtcgccttcg tcatccttgc cgtacaggcg ctcgccctcg
      541 cgcaggcggc gcggcacggc cgactcgagc gcctcgtcgc gcagggcctc gggcagctcc
      601 gcgaaccagt cgttggcgag gagcgtcgcg cgttgcgccg gggtgagcca ggccatggtg
      661 ttttcccttc gtcagaatgc caatccttga cagacgtcgc gcagggtccg tcgctagcat
      721 ccgacgcacc gggggcccga ggagccgaat cttcgatccg cccgcgcgcg ctgcctatcc
      781 gcagtgctcc ccatgccatc gcgccggtct tccgtgctat ccgcacgccg cccgcgcccg
      841 ccgaaagcca cccgcgacct gagtcgacgc gagatcgaca cgggagggtc acccaggcgg
      901 gccgtcctcg cggccctgcg acgagaccac tcagaaacgc atggagaaat catgagaagc
      961 acccttgcca aatggctggc cgtggccgcc atcgcgctgt ccgccctgcc cctgcccgca
     1021 tgggccacgg ggtatgcgca gacgaagtac cccatcgtgc tcgtgcacgg cctgttcggc
     1081 ttcgacaaca tcgggccgct cgagtatttc tacggcatcc ccgaggacct gcgcgccaac
     1141 ggcgccaccg tctacgtcgc gcaggtctcg gccgccaact ccaccgaggt gcgcggcgag
     1201 cagctgctga cctacgtcaa gcaggtgctg gcggtcaccg gcgcgaagaa ggtcaacctg
     1261 atcggccaca gccacggcgg cccgacggtg cgctacgtcg cgtcggtcgc gccgcagctg
     1321 gtggcgtcgg tgaccagcgt ggccggcccg aaccagggcg cggcactcgc ggatgcgctc
     1381 aacggcacgc tgccggcagg ctcgatctcg cgcacggtgg tcgccggcgc ggtcaacgcg
     1441 ttcgccgcca tcatcgagct cgtctcgggt gatgccggcg caccgcagaa ctcctcggcg
     1501 gcgctgaatt cgctgtcgtc ggcaggcatg gccaagttca acgcgtcgca tcccgagggg
     1561 ctgcccacct cctcctgcgg ccagggcccg tccacggtca acggcgtgcg ctactggtcg
     1621 tggagcggcg cgcagcccta caccaacgtg ctcgacccga tcgacccgtc gctcgcgctg
     1681 acctcgctgg tctacggcgg cgcgaagaac gacggcctcg tgggcagctg cagcagccac
     1741 ctcggcacgg tgctgcgcga cgactacgcg atgaaccacc tcgacgaggt gaaccagacc
     1801 gccggcctcg tcaacctgtt cgagaccaac ccgaagacgg tcttccgcga ccacgccaac
     1861 cgcctgaaga acgcgggcct ttgagatgga caagcgcctg tccatggcgc tggtgaccgc
     1921 ggcgctggcc gccgcggcgc tgatcgccgt ctccgaatgg cgcgcgcgcc tcgcgccgtc
     1981 cccgcccgtc
//
LOCUS       DASVLN010000430         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_183222, whole genome shotgun sequence.
ACCESSION   DASVLN010000430 DASVLN010000000
VERSION     DASVLN010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183222"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VF453_10575"
     CDS             complement(<1..335)
                     /locus_tag="VF453_10575"
                     /inference="COORDINATES: protein motif:HMM:NF042671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HEX7688145.1"
                     /translation="MATFLADVATHTPTWAWIALAVLVALGVRSMRPNDMSVQRLRLV
                     PVVMGAYSLLGTCNAFQQVGPGAAAVAWAAGAVLGFAANRVLDLPRGVTANPDGTVRL
                     AGSVAPLVL"
     gene            543..1310
                     /locus_tag="VF453_10580"
     CDS             543..1310
                     /locus_tag="VF453_10580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7688146.1"
                     /translation="MNQESSPGAGAEALAPHAPLTAYYADEAERHRFLRRIFDQTAPD
                     YDRIERLLAFGTGPWYRRQALLRAGLAAGARVLDVGIGTGLVAREALVVAGPSCALVG
                     VDPSVGMMGEVHLPRVELREGRAEALPVEDAGFDFLSMGYALRHVSDVAAAFAEFHRA
                     LRPGGRLLVLEITRPESRLGRAALKAYMRGLVPLLARMVGRGRHTPELWRYYWDTIEA
                     CMAPAAVLQALRDAGFVDVRRRVELGMFSEYTARKLA"
     gene            1358..2689
                     /locus_tag="VF453_10585"
     CDS             1358..2689
                     /locus_tag="VF453_10585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7688147.1"
                     /translation="MPAQRATCDVLVIGGGPAGSTIAALLAREGRDVVVLEKARHPRF
                     HVGESLLPANVALFDRLGLRAEVERIGMPKWGVEFVSPDHAHQSLLEFGDAWDKTMPY
                     AWQVRRSELDEILFRNAATQGARTLEGARVDDVRFDADGATVCARLEGGGRGEWRARF
                     VVDASGRDTFLANRLKSKKKIAGHNSAAMFGHFHGAQRLPGKLEGNISIFWFEHGWFW
                     FIPLADGTTSVGAVCWPYYLKSRAKPMREFFADTIALCPQLAERLQGATLVDDAVHAT
                     GNYSYGGTACAGERFLLLGDAFAFVDPVFSSGVYLAMHNAFEGAKVVADTLDRPAQAA
                     RARRAFERYMRRGPREFSWFIYRVTNPTMREFFMYPRNPMRVKEALLSLLAGDIYDDT
                     PLWPSLYAMKGLYFLFSALHPKRTWAAWRRRRRNVRDFGMLDGETIAPVRG"
BASE COUNT          375 a         1033 c         1043 g          385 t
ORIGIN      
        1 aacagcacca gcggcgcgac gctgccggcc aggcgcacgg tgccgtccgg attggcggtg
       61 acgccgcgcg gcaggtcgag cacgcggttg gccgcgaagc cgagcacggc gcccgctgcc
      121 cacgcgacgg cggccgcgcc agggccgacc tgctggaacg cgttgcaggt gcccagcagc
      181 gagtacgcgc ccatgacgac gggcacgagt cgcaggcgtt ggaccgacat gtcgttgggc
      241 cgcatcgagc gcacgcccag cgcgacgagg acggcgaggg cgatccaggc ccaggtcggg
      301 gtgtgggtgg cgacgtcggc gaggaaggtg gccatggagt ccttgcggtg gtgttgaagg
      361 gatggccgcc atggtcgggc ggcgcggcgc ccgcgtcatc gggcatgcga ccggacgcgg
      421 tccggcggcg cgaaacgtcg cctcggcggc gcgtgacgga tgccgcggac ggaggctttc
      481 ggcactgccg caccccccat tggcgggacg gacgcggcat gcgctgttgg cataatccgc
      541 ggatgaacca agagagttcc ccgggcgccg gagcggaggc gttggccccg catgcaccgc
      601 tgaccgccta ctacgcggac gaggcggaac gccaccgctt cctccgtcgc atcttcgacc
      661 agaccgcgcc cgactacgac cgcatcgaac gcctgctcgc gttcggaacg gggccgtggt
      721 accggcgcca ggcactgctg cgcgcgggcc tggccgccgg cgcgcgggtg ctggacgtgg
      781 ggatcggcac cgggctcgtg gcgcgcgagg cgctggtcgt cgccgggccg tcgtgcgcgc
      841 tggtcggcgt cgaccccagc gtcggcatga tgggcgaagt gcacctgccc cgcgtcgagc
      901 tgcgcgaggg ccgggccgag gcgctgccgg tcgaggacgc gggcttcgac ttcctcagca
      961 tgggctacgc gctgcgccac gtgtccgacg tggcggcggc gttcgccgaa ttccaccgcg
     1021 cgctgcgtcc gggcggccgc ctgctggtgc tggagatcac gcgcccggag agccgcctcg
     1081 gccgagccgc gctcaaggcc tacatgcgcg gcctcgtgcc gctgctcgcg cgcatggtcg
     1141 gccgcggccg ccacacgccc gagctgtggc gctactactg ggacacgatc gaggcctgca
     1201 tggcgcccgc cgcggtgctg caggcgctgc gcgacgccgg cttcgtcgac gtccggcgcc
     1261 gcgtcgagct gggcatgttc tccgaataca cggcgcgcaa gctcgcatga cgatcgccac
     1321 gccatcgacc gccgaggcgc cgcgcgggag cccggccatg cccgcgcagc gcgcgacttg
     1381 cgatgtcctc gtgatcggcg gcggcccggc cggctcgacg atcgcggcgc tgctcgcccg
     1441 cgagggccgc gacgtcgtcg tgctcgagaa ggcgcgccac ccgcgcttcc acgtcggcga
     1501 gtcgctgctg ccggccaacg tcgcgctgtt cgatcgcctg ggcctgcgcg ccgaggtcga
     1561 gcgcatcggc atgcccaagt ggggcgtcga gttcgtctcg cccgaccatg cgcaccagag
     1621 cctgctcgaa ttcggcgacg cctgggacaa gacgatgccc tacgcgtggc aagtgcggcg
     1681 ctcggagctc gacgagatcc tgtttcgcaa cgccgcgacg cagggcgcgc gcacgctgga
     1741 gggcgcgcgc gtcgacgacg tgcgcttcga cgccgacggc gcgacggtct gcgcgcgcct
     1801 ggagggcggc ggccgcggcg agtggcgcgc gcgcttcgtc gtcgacgcct cgggccgcga
     1861 caccttcctc gccaaccgcc tgaagtccaa gaagaagatc gcgggccaca acagcgccgc
     1921 gatgttcggc cacttccacg gcgcgcagcg gctgcccggc aagctcgagg gcaacatctc
     1981 gatcttctgg ttcgagcacg gctggttctg gttcatcccg ctggccgacg gcacgaccag
     2041 cgtcggcgcc gtctgctggc cgtactacct caagtcgcgg gccaagccga tgcgcgagtt
     2101 cttcgccgac acgatcgcgc tgtgcccgca gctcgccgag cgcctgcagg gcgcgacgct
     2161 ggtggacgac gcggtgcacg cgacgggcaa ctactcgtac ggcggcacgg catgcgccgg
     2221 cgaacgcttc ctgctgctgg gcgacgcgtt cgcgttcgtc gacccggtgt tctcgtcggg
     2281 cgtgtacctc gcgatgcaca acgccttcga gggcgccaag gtggtcgccg acacgctgga
     2341 ccgaccggcg caggcggcgc gcgcgcgccg cgccttcgag cgctacatgc gccgcggccc
     2401 gcgcgagttc tcgtggttca tctaccgcgt caccaacccg acgatgcgcg agttcttcat
     2461 gtacccgcgc aacccgatgc gggtcaagga ggcgctgctg tcgctgctgg ccggcgacat
     2521 ctacgacgac acgccgctgt ggccctcgct gtacgccatg aagggcctgt acttcctgtt
     2581 ctcggcgctg catccgaagc gcacctgggc ggcctggcgc cgccgccgcc gcaacgtgcg
     2641 cgacttcggg atgctcgacg gcgagacgat cgcccccgta cgcggatgag cgcccgcccg
     2701 gccgcccgaa gggcgctcgc accgcaggcc gccaggccgg aggtttcgtc atggacgagc
     2761 gcccgcccgg ccgcccgaag ggcgctcgca ccgcaggccg ccaggccgga ggtttccccg
     2821 tgaccgcccg cccgtc
//
LOCUS       DASVLN010000431         4386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_183308, whole genome shotgun sequence.
ACCESSION   DASVLN010000431 DASVLN010000000
VERSION     DASVLN010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183308"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..676)
                     /locus_tag="VF453_10590"
     CDS             complement(32..676)
                     /locus_tag="VF453_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688148.1"
                     /translation="MTPAAATPSATADKLETLLVVVRESPGADKRDEALVPMAKLQVD
                     LPGGIKAEMSPAWFELIGDLHVRLVFDQGETLRTVRTEELAELGLTPEAAIARGLANL
                     ERLHGAPESVPWHGLMRVQGRAEDFASSYFLDRGFWRALLERHPEGLVVAVPQHGGLL
                     WTPAGDAQGVATLESRVGALHDAGQAQRLSTAVYLFKDDRWTVRRPARPAPPAN"
     gene            complement(820..1218)
                     /gene="arfB"
                     /locus_tag="VF453_10595"
     CDS             complement(820..1218)
                     /gene="arfB"
                     /locus_tag="VF453_10595"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX7688149.1"
                     /translation="MPLHIPPEEVEITAIRAQGSGGQNVNKVSTAVHLRFDVRASSLP
                     EGVKARLLALRDHRITDEGVVVIKAQQHRSQELNRADALARLRALVDAAEKPPLKRRP
                     TRPTLGSQRRRLEGKSVRSAIKAGRGRVDV"
     gene            1429..4203
                     /gene="acnA"
                     /locus_tag="VF453_10600"
     CDS             1429..4203
                     /gene="acnA"
                     /locus_tag="VF453_10600"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX7688150.1"
                     /translation="MPLAQPAPHAPFAHALQTFTMPSGATGRFHSIPALAQEFPQVAR
                     LPVSLRIVLEAVLRHCDGRRVMPEHVRQLAAWKPNGERSEEIPFVVARVVLQDFTGVP
                     LLADLAAMRSEARNQGADPKTIEPLVPVDLVVDHSVMIDDFGHKDSLDINMKLEFQRN
                     NERYKFMKWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHRQADAQHPDRPPVYYPDT
                     LVGTDSHTTMINGLGVVGWGVGGIEAEAAMLGQPVYFLTPDVVGFEFTGRLREGVTAT
                     DLVLTVTEMLRKAKVVGKFVEFFGEGVAQLSVPDRATMANMAPEYGATMGFFAVDDKT
                     VEYLRGTGRTAEEIEAFEAYFRAQGLFGTPASGSIDYSQTLTLDLGTVAPSLAGPKRP
                     QDRIEIGHLAKKFEELLVTPAASNGYNLAAEQTLRTAALPNGTRIGHGDVLIAAITSC
                     TNTSNPSVMLAAGLLAKKAVAAGLSVQPHVKTSLAPGSRIVTEYLTKAGLIEPLARLG
                     FNVAAYGCTTCIGNAGDLAPEINQAIVDNDLVCAAVLSGNRNFEARIHPNLKANFLAS
                     PPLVVAYAIAGNVKRDLMTEPVGRNAQGKDVWLGDIWPTSDEVHALLRFAMDADAFRA
                     NYGKVKSAPGALWNDITGVTGQVYDWPTSTYIARPPFFDGFSRQPKPAPTAVKGAKIM
                     ALFGDSITTDHISPAGSIKADSPAGKYLQSHGVLPADFNSYGSRRGNHDVMMRGTFAN
                     VRIKNLMLPPRADGSRVEGGYTLFRGTPVAELPADGTTPVFIYDAAMAYQAQGTPTVI
                     FAGEEYGTGSSRDWAAKGTQLLGVKAVVARSFERIHRNNLVGMGVLPLQFKPGESWTS
                     LGITGDETIDIEWEAPITPQSEATLVIHRPDGKSQRVKLTLRIDTPIEVDYYAHGGIL
                     PYVLRQLLAA"
BASE COUNT          662 a         1600 c         1538 g          586 t
ORIGIN      
        1 cgcgtcccgg gcgtcccggc gcggcgcgcg gtcagttcgc cggcggcgcc gggcgcgccg
       61 gccgccgtac cgtccagcgg tcgtccttga acaggtagac ggccgtcgac aaccgctgcg
      121 cctggcccgc gtcgtggagc gcgccgacgc gcgattccag cgtcgcgacg ccctgcgcat
      181 cgcccgccgg cgtccacagc aggccgccgt gctgcggcac ggcgacgacg agcccctcgg
      241 gatggcgctc gagcagcgca cgccagaagc cgcggtcgag gaaatagctg ctcgcgaagt
      301 cctccgcgcg cccctgcacg cgcatcaggc cgtgccaggg caccgattcc ggcgcgccgt
      361 gcagccgctc caggttggcc aggccgcggg cgatggcggc ctcgggagtc aggccgagct
      421 cggcaagctc ctcggtgcgc acggtgcgca gcgtctcgcc ctggtcgaac accagccgca
      481 cgtgcaggtc accgatcagc tcgaaccacg ccggcgacat ctccgccttg atcccgcccg
      541 gcaggtcgac ctgcagcttg gccatcggca ccagggcctc gtcgcgcttg tccgcgccgg
      601 gcgactcgcg caccacgacg aggagggttt ccagcttgtc ggcggtggcg gacggcgtgg
      661 cggcggcagg ggtcatggcg atcgcggcga gcagggcaag gaaagggcgg cggtgcacgg
      721 caggacggga ttgtcggccg acgcggccgg ccccggagtg ttccacggtc gggcggcgtc
      781 ggccatcccg ccgccggggg ggcgcccgcc ggcacgagct caaacgtcca cccgaccgcg
      841 cccggccttg atcgccgagc gcacgctctt gccctccagc cgccgccgtt gggagccgag
      901 cgtcgggcgc gtcgggcggc gtttcagcgg cggcttctcg gccgcgtcga ccagcgcccg
      961 cagccgcgcc agcgcgtcag cacggttgag ctcctggctg cggtgctgtt gcgccttgat
     1021 gacgacgacg ccctcgtcgg tgatgcggtg gtcgcgcagc gccagcaggc gcgccttgac
     1081 gccctcgggc agcgaggacg cgcgcacgtc gaagcgcagg tgcacggcag tggagacctt
     1141 gttcacgttc tgcccgccgc tgccctgggc gcggatcgcg gtgatctcga cttcctccgg
     1201 cgggatgtgc agcggcatgg ggcgattgtc gccgccccac gcgccggcgc acggatgagc
     1261 ggacagcccg catgaccagc cgatgcgtcg gacagccctc cggccgccgc cgcagcggcg
     1321 atcccgagta aaagcgactg gcactcgccc gcgttcttga ggcggacatc gcccgggtgg
     1381 caacatcgca ggctgcctgc accccaacgc cactcgcgag gagatcccat gcccctggct
     1441 cagcccgcgc cgcacgcccc gttcgcccac gccctgcaga ccttcacgat gccgtcgggg
     1501 gcgaccggcc gcttccactc gatccccgcg ctcgcgcagg aattcccgca ggtcgcgcgc
     1561 ctgccggtgt cgctgcgcat cgtcctggag gccgtgctgc gccactgcga cggccggcgc
     1621 gtgatgcccg agcacgtgcg ccagctcgcg gcgtggaagc ccaacggtga gcgcagcgag
     1681 gagatcccct tcgtcgtcgc gcgcgtcgtg ctgcaggact tcaccggcgt gccgctgctg
     1741 gccgacttgg cggcgatgcg ctccgaggcg cgcaaccagg gcgccgaccc gaagacgatc
     1801 gagccgctgg tgccggtcga cctggtcgtc gaccacagcg tgatgatcga cgacttcggc
     1861 cacaaggact cgctcgacat caacatgaag ctcgagttcc agcgcaacaa cgagcgctac
     1921 aagttcatga agtggggcat gcaggcgttc gacaccttcg gcgtcgtgcc gccgggcttc
     1981 ggcatcgtgc accaggtcaa cctggagtac ctcgcccgcg gcgtgcaccg gcaggccgac
     2041 gcgcagcatc ccgaccggcc gccggtctac tacccggaca ccctggtggg taccgacagc
     2101 cacaccacga tgatcaacgg cctgggcgtg gtcggctggg gcgtcggcgg catcgaggcc
     2161 gaggccgcga tgctgggcca gccggtctac ttcctcacgc cggacgtcgt gggcttcgag
     2221 ttcaccggcc gcttgcgcga gggcgtcacg gcgaccgacc tcgtgctgac ggtcaccgag
     2281 atgctgcgca aggccaaggt ggtcggcaag ttcgtcgagt tcttcggcga gggcgtggcg
     2341 cagctatcgg tgcccgaccg cgcgaccatg gccaacatgg cgcccgagta cggcgcgacg
     2401 atggggttct tcgccgtcga cgacaagacg gtcgagtacc ttcgcggcac cggccgcacg
     2461 gccgaggaga tcgaagcctt cgaggcctac ttccgcgcgc aaggcctgtt cggcacgccg
     2521 gcatcggggt cgatcgacta ctcgcagacg ctgaccctgg acctcggcac cgtcgcgccc
     2581 agcctcgcgg gcccgaagcg gccgcaggac cgcatcgaga tcggacacct cgcgaagaag
     2641 ttcgaggagc tgctggtcac ccccgcggcc agcaacggct acaacctggc ggccgagcag
     2701 acgctgcgca ccgcggcgct gcccaacggc acgcgcatcg gccacggcga cgtgctgatc
     2761 gcggcgatca cgagctgcac gaacacgagc aacccgagcg tcatgctggc tgccggcctg
     2821 ctggcgaaga aggcggtcgc cgccgggctc tcggtgcagc cgcacgtcaa gacctcgctg
     2881 gcgcccggct ctcgcatcgt caccgagtac ctgaccaagg cgggcctgat cgagccgctc
     2941 gccaggctgg gattcaacgt cgcggcctac ggctgcacca cctgcatcgg caacgccggc
     3001 gacctcgcgc cggagatcaa ccaggccatc gtcgacaacg acctcgtgtg cgcggcggtg
     3061 ctctcgggca atcgcaactt cgaggctcgc atccatccga acctgaaggc caacttcctc
     3121 gccagcccgc cgctggtcgt ggcctatgcg atcgccggca acgtcaagcg cgacctgatg
     3181 acggagccgg tgggccgcaa cgcgcagggc aaggacgtgt ggctgggcga catctggccg
     3241 acctccgacg aggtccacgc gctgctgcgc ttcgcgatgg acgccgacgc cttccgcgcc
     3301 aactacggca aggtgaagtc ggcgccgggc gccctgtgga acgacatcac gggcgtgacg
     3361 ggccaggtct acgactggcc gaccagcacc tacatcgcgc ggccgccgtt cttcgacggc
     3421 ttctcgcgcc agccgaagcc ggcgccgacc gcggtcaagg gcgcgaagat catggcgctg
     3481 ttcggcgact cgatcacgac cgaccacatc tcgccggccg gctcgatcaa ggcggactcg
     3541 cccgccggca agtacctgca gtcgcacggc gtgctgccgg cggacttcaa ctcctacggc
     3601 tcgcgccgcg gcaaccacga cgtgatgatg cgcggcacct tcgccaacgt gcgcatcaag
     3661 aacctgatgc tgccgccgcg cgcggacggc agtcgggtcg agggcggcta cacgctgttc
     3721 cgcggtacgc cggttgcgga gctgccggcc gacggcacga cgccggtctt catctacgac
     3781 gccgcgatgg cctaccaggc gcagggcacg ccgacggtga tcttcgcggg cgaggaatac
     3841 ggcaccggct ccagccgcga ctgggcggcc aagggcacgc agctgctggg cgtgaaggcc
     3901 gtcgtggcgc gcagcttcga gcgcatccat cgcaacaacc tggtcggcat gggcgtgctg
     3961 ccgctgcagt tcaagccggg cgagagctgg acgagcctcg gcatcaccgg cgacgagacg
     4021 atcgacatcg agtgggaggc gccgatcacg ccgcagtccg aggcgacgct ggtcatccac
     4081 cgtccggacg gcaagagcca gcgcgtgaag ctgacgctgc gcatcgacac cccgatcgag
     4141 gtcgactact acgcgcacgg cggcatcctg ccgtacgtgc tgcgccagct gctcgccgcc
     4201 tgagcgcaag cgcgcgcgag cggccggccg gggttcaggc gccggccggg aagtcggtgg
     4261 acacgccgag cgcacgccag cggtcgagct cggcgcgggt ctcggcgagc ttggcgtcca
     4321 tcgccgcgat cgcctgggcg ccggccacga agcgcagcgg tggccgcggc gcctcggcca
     4381 actgca
//
LOCUS       DASVLN010000432         6114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_236076, whole genome shotgun sequence.
ACCESSION   DASVLN010000432 DASVLN010000000
VERSION     DASVLN010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236076"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VF453_10605"
     CDS             <1..456
                     /locus_tag="VF453_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688151.1"
                     /translation="NARTPGPVRAPRGPASNTRPRKTLAMWLIGLVVLLLVMVVPVMI
                     GARIVGAKRTGFWMALLAILVAGLITGIALHVFRGGGLLSLLVDALAYMLILDTTYLR
                     GLAIALIQWVLTFVFAAIVAIVFFGSMAAGMHKLMHDAPFPIDLPAQHV"
     gene            complement(522..929)
                     /gene="yiaA"
                     /locus_tag="VF453_10610"
     CDS             complement(522..929)
                     /gene="yiaA"
                     /locus_tag="VF453_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HEX7688152.1"
                     /translation="MSHAAPKPTAVFVAASWLSLGVGLVAYCAGLWNARMALDEKGLY
                     LTMLMFGLFAAVSLQKTVRDRMEGLPVTGLYVGLCWGALLACVVLLATSLWNSGLPVD
                     EQGFYAMAFVLSLFATVAIQKNVRDAAHPAPVQ"
     gene            complement(1067..2110)
                     /locus_tag="VF453_10615"
     CDS             complement(1067..2110)
                     /locus_tag="VF453_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7688153.1"
                     /translation="MRALTLESRPRIGWGLLGAGGISDQLARALSYVPDARLVAVGSR
                     DAAKARAFIDAPRGGESPREPVRAHGSYRALVEDPEVDVVYIGTPHPDHAPSMALALE
                     AGKAVLCEKPFTVNRRQAEAAVALARRRGVFLMEAMWTRYVPAMVELKRLVDAGAIGE
                     VVSTTADFGFAATGLPAEHRALNPALAGGGLLDVGIYPLSFAAFVLGPIAAVQADAEL
                     GPTGVDVHTVFNLRHANGRLSQGLCSLRSTTPGLAAVLGTHGRIDVAPPFYAAQRLEL
                     RRGTGPWEEQPVEVLDRPWRGNRYVPQIEEVHRCLRAGLAESPAMPLDESVALVGWMD
                     TMRAQFGLRYPGE"
     gene            complement(2144..2440)
                     /locus_tag="VF453_10620"
     CDS             complement(2144..2440)
                     /locus_tag="VF453_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7688154.1"
                     /translation="MTTVLFHPDADSEFTEELEYYSETRDGVVGRFRESVEQAVARAA
                     ANPRGGKPSFGQTRAMRVKGFPFDVIYLESFDELFVVAIAGHRRSPGYWLPRLP"
     gene            complement(2437..2667)
                     /locus_tag="VF453_10625"
     CDS             complement(2437..2667)
                     /locus_tag="VF453_10625"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX7688155.1"
                     /translation="MQDLVSELARLGRTLPLEDRERLVDELLRSISEDSPSEIDPAWE
                     AEIERRVTEIKQGKVELLDARDVFARARHRFG"
     gene            complement(2763..6017)
                     /locus_tag="VF453_10630"
     CDS             complement(2763..6017)
                     /locus_tag="VF453_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7688156.1"
                     /translation="MAEATPNKEARQRLRQALTRHLLAATAGVASASALMFLVAGAPL
                     DWAARLWLATAFVGLAGVAFALRRDGGIGTQVRALPYVIGTAIGLSGVAAAVAGEGLM
                     SPALAVVGVLLCLFTVVGGLRRGLLAAAIGVGMLVLLAVATALDWITAPGLAPGMPVF
                     VLARPLLTHALALGIALGGGELLGRMLDRTLRSADERERRFLGLLAVAADAYWEMDGS
                     GRLVRFSAKEELRNFVGLRRHGVDAGMGKLLWETRGVAFDGEVLETFRSELAARLPMR
                     DVPLRWSDVAGRVRHFTLSAEPRTNEAGKFAGYWGVLRDITQDVKARQALWSTETRYQ
                     DLFRRIPTPLVLHRETRVLDANPAGVALFGFGDLRSMLGQDLVGLVEAGESRERALAH
                     ARALEALPVGEGLPEAEFTLRPSPERLLTVRMNSVRVDVDGEAATLSIIIDDTERRAA
                     EDLVRRSETLLSHLVSTSPDWITLTEYPSGRYAMVNPTFLATTGHAAEDVMGRTPAEL
                     GLWADPVQRDRISRLLEQHQAVQNVPATMLTRDGRRVSMLVSAARFTIDDVDYQMTSA
                     RDVTATEQARREREAILENALVGISFTRDRRIVMTNARFDEMFGWPRGSMQNQPQRMV
                     WPSDEAYGDMLARVGPALARGEQVDLETPMMRRDGRTFTGRLLAKALDPTQPASGTIW
                     LAEDITERRAVEQALARARDEAEAANRAKSAFLANTSHEIRTPLNALVGLAQLARTPE
                     VDDRRRLQYIEQICDSADTLSKILSDILDLSKIEAGKLIVDRVAFDLHALVDTLRQAY
                     GALADVKGLALRVDLEPGVPAHVLGDPVRVRQILSNYLTNALKFTNRGSIVLNVQRAV
                     GHRIRFEVIDTGEGMDAAGQAMLFLPFSQVDNSITRKVGGTGLGLSICSELARLMDGT
                     VGVSSEPGKGSMFWVELPMPAVAQAHVDTHVSSSGLDALLGTRVLMVEDNPVNMMIAV
                     ALLERWGAIVDQADDGAGALHAVDHAHQSGNPFDIVLMDVQMPGMSGHEVTRRLRQRY
                     DRRALPIIALTAAALVSEREDAMAAGMNDFLTKPIDAERLRQTLQRVLAEKAPG"
BASE COUNT          859 a         2219 c         2171 g          865 t
ORIGIN      
        1 aatgcgcgca cgccgggccc ggtgcgcgcg ccgcgcggcc cggcctccaa cactcgacca
       61 agaaagaccc tcgccatgtg gctcatcggc ctcgtcgtcc tcctgctcgt catggtcgtg
      121 cccgtgatga tcggcgcccg catcgtcggc gcgaagcgca ccggcttctg gatggcgctg
      181 ctggccatcc tcgtggccgg cttgatcacg ggcatcgcgc tgcacgtctt ccgcggcggc
      241 ggcctgcttt cgctgctggt cgacgcgctc gcctacatgc tgatcctgga cacgacctac
      301 ctgcgcggcc tcgcgatcgc gctgatccag tgggtgctga ccttcgtctt cgccgcgatc
      361 gtcgcgatcg tcttcttcgg cagcatggcc gccggcatgc acaagctgat gcacgacgcg
      421 ccgttcccga tcgacttgcc ggcgcagcac gtgtagtcaa tcgccgtcgg gcagcccgcc
      481 gtcgcgccag gcgcgcgcgg cgcgagacgc cgcgcgggcg ttcattgcac cggcgccgga
      541 tgcgccgcgt cgcgcacgtt cttctggatc gcgaccgtcg cgaacaggct cagcacgaaa
      601 gccatcgcgt agaagccctg ctcgtcgacc ggcaggccgg agttccacag gctcgtggcg
      661 agcagcacca cgcaggcgag cagcgcgccc cagcacaggc cgacgtacag gccggtcacc
      721 ggcaggccct ccatgcggtc gcgcacggtc ttctgcagcg agacggccgc gaacaggccg
      781 aacatcagca tcgtcaggta caggcccttc tcgtcgaggg ccatgcgcgc gttccagagg
      841 ccggcgcagt aggcgacgag gcccacgccc aggctgagcc aggaggcggc gacgaagacg
      901 gcggtgggct tgggggcggc gtggctcacg gatgattcct tcgaacatgg cgcgacggct
      961 cgtcgcgtgg cgccattctg ggatcgccgg gcgagcccgt ggggcgcgcc cgcggcagtc
     1021 gtggccggcc ggcccggcgg tatcgcgagg cgctaccgcc ggccggtcat tcgcccggat
     1081 accgtagacc gaactgcgct cgcatcgtgt ccatccagcc gacgagggcg accgactcgt
     1141 ccagcggcat cgcgggactt tcggccaggc cggcgcgcag gcagcggtgc acctcctcga
     1201 tctgcgggac gtagcggttg ccccgccagg ggcgatcgag cacctcgacg ggctgctcct
     1261 cccacggccc ggtgccgcgg cgcagctcca gtcgctgcgc cgcgtagaac ggcggcgcga
     1321 cgtcgatgcg cccgtgggtg ccgagcaccg ccgcgaggcc gggcgtggtc gagcgcagcg
     1381 agcacaggcc ctggctcagg cgcccgttgg cgtggcgcag gttgaagacg gtgtggacgt
     1441 ccacgcccgt cggcccgagc tccgcgtccg cctgcaccgc cgcgatcggc cccagcacga
     1501 aggccgcgaa gctgagcggg tagatgccga cgtccagcag gccgccgccc gcgagcgccg
     1561 gattgagcgc gcgatgctcc gccggcaggc cggtggccgc gaagccgaaa tcggccgtcg
     1621 tcgagacgac ctcgccgatc gcgccggcgt cgacgagccg cttgagctcc accatcgccg
     1681 gcacgtagcg cgtccacatc gcctccatca ggaagacgcc gcgccgccgc gccagcgcca
     1741 ccgcggcctc tgcctggcga cggttgacgg tgaacggctt ctcgcacagc acggccttgc
     1801 cggcctccag cgcgagcgcc atcgacggcg cgtggtccgg atgcggcgtc ccgatgtaga
     1861 cgacgtcgac ctccggatcc tcgaccagcg cgcggtagct gccgtgcgcg cgcacgggct
     1921 cgcgcgggga ctcgccgcca cgcggcgcgt cgatgaacgc ccgtgccttg gccgcgtccc
     1981 gcgagccgac ggccacgagc cgcgcgtccg gcacgtacga caacgcgcgc gccagctggt
     2041 cggagatgcc gccggcgccg agcaggcccc agccgatgcg ggggcgggat tcgagggtca
     2101 gtgcgcgcat gtgttcagac gaagtcgttt cgcggggaag tcttcaaggc agacgcggca
     2161 accagtagcc gggactgcgt cggtggccag cgatcgcgac gacgaacagt tcgtcgaagg
     2221 attccagata aatgacgtcg aacggaaagc ccttgacgcg catcgcgcgg gtctgcccga
     2281 aagaaggctt gcccccgcgg ggattcgcgg ccgctcgggc gaccgcttgc tcgacgcttt
     2341 cgcggaacct tccgacgacc ccgtcacgcg tttcgctgta gtactccagc tcctcggtga
     2401 actcgctgtc cgcgtcgggg tggaagagga ccgtcgtcat ccgaatcggt gacgcgccct
     2461 tgcgaagaca tcccgggcgt cgagcaattc caccttgccc tgcttgatct ccgtgactct
     2521 gcgctcgatc tccgcctccc aggccggatc gatctcggac ggagaatcct cgctgatcga
     2581 gcgaagcagc tcgtccacga gacgctcgcg gtcctccagc ggcaacgtac ggccgaggcg
     2641 ggcgagttcg gatacgaggt cttgcatggt ggcggctcct tggtaccggg tcagtctaac
     2701 gccgccgccg gcgatgggcg cgcgtcgaat gccgcggtaa cggggcgctt tggcgcgggg
     2761 cgtcaacctg gcgccttctc ggccagcacc cgctgcagcg tttgccgcag gcgctcggcg
     2821 tcgatcggct tggtcaggaa gtcgttcatg cccgcggcca tcgcgtcctc gcgctcggag
     2881 accagcgcgg cggcggtcag cgcgatgatg ggcagcgcgc ggcggtcgta gcgctggcgc
     2941 aggcggcgcg tgacctcgtg gccgctcatg ccgggcatct ggacatccat cagcacgatg
     3001 tcgaacgggt tgccggactg gtgcgcatga tcgacggcgt gcagcgcgcc cgcgccgtcg
     3061 tcggcctggt cgacgatcgc gccccagcgc tccagcagcg cgacggcgat catcatgttg
     3121 acggggttgt cctcgaccat cagcacgcgc gtgcccagca gggcgtccag gcccgaggag
     3181 ctcacgtgcg tgtcgacatg cgcctgggcg accgcgggca tcggcagctc gacccagaac
     3241 atgctgccct tgccgggctc gctggagacg cccacggtgc cgtccatcag ccgcgccagc
     3301 tcgctgcaga tcgacagccc gagcccggtg ccgccgacct tgcgcgtgat cgagttgtcg
     3361 acctggctga acggcaggaa cagcatcgcc tgtcccgcgg cgtccatgcc ttcgccggtg
     3421 tcgatcacct cgaagcggat gcggtggccg accgcgcgct gcacgttcag cacgatgctg
     3481 ccgcggttcg tgaacttcag ggcgttggtg aggtagttgc tgaggatctg ccgcacccgc
     3541 accggatcgc ccagcacgtg cgccggcacg ccgggctcga ggtcgacgcg cagcgcgagg
     3601 cccttgacgt cggccagcgc gccgtaggcc tggcgcagcg tgtcgacgag cgcgtgcagg
     3661 tcgaacgcga cccggtcgac gatcagcttg cccgcctcga tcttggacag gtcgaggatg
     3721 tccgacagga tcttggacag cgtgtcggcc gagtcgcaga tctgctcgat gtactgcagg
     3781 cgccggcggt cgtcgacctc gggcgtgcgc gccagctgcg cgaggcccac gagcgcgttc
     3841 agcggcgtgc ggatctcgtg gctcgtgttg gcgaggaacg cgctcttggc gcggttggcg
     3901 gcttccgcct cgtcgcgcgc gcgggcgagc gcctgctcca ccgctcggcg ctcggtgatg
     3961 tcctcggcca gccagatcgt gccgctggcg ggctgggtcg ggtcgagcgc cttggccagc
     4021 agccgaccgg tgaaggtgcg gccgtcgcgg cgcatcatcg gcgtctcgag gtcgacctgc
     4081 tcgccccgcg cgagcgccgg cccgacgcgc gccagcatgt cgccataggc ctcgtccgac
     4141 ggccacacca tgcgctgcgg ctggttctgc atcgagccgc gcggccagcc gaacatctcg
     4201 tcgaagcgcg cgttggtcat gacgatgcgg cggtcgcgcg tgaacgagat gcccaccagc
     4261 gcgttctcca ggatcgcctc gcgctcgcgc cgcgcctgct cggtggccgt gacgtcgcgg
     4321 gcgctggtca tctggtagtc gacgtcgtcg atcgtgaagc gcgcggccga caccagcatc
     4381 gacacgcggc gtccgtcgcg cgtgagcatc gtcgcgggga cgttctgcac cgcctggtgc
     4441 tgctccagca ggcgcgagat gcggtcgcgc tgcaccggat cggcccagag ccccagctcg
     4501 gcgggcgtgc ggcccatgac gtcctcggcc gcgtggccgg tggtcgcgag gaaggtcggg
     4561 ttgaccatcg cgtagcggcc gctcgggtac tcggtgagcg tgatccagtc ggggctggtg
     4621 gagacgaggt gcgacagcag cgtctcgctg cggcgcacca ggtcctcggc cgcgcgacgc
     4681 tcggtgtcgt cgatgatgat ggacagcgtc gcggcctcgc cgtcgacgtc cacgcgcacg
     4741 ctgttcatcc gcacggtcag caggcgctcc ggcgacgggc gcagcgtgaa ctcggcctcg
     4801 ggcaggccct cgccgaccgg cagcgcctcg agcgcgcggg cgtgcgccag cgcgcgctcg
     4861 cgcgattcgc ccgcctcgac gagccccacg aggtcctgcc ccagcatcga gcgcaggtcg
     4921 ccgaagccga acagcgcgac gcccgccgga ttggcgtcga gcacgcgcgt ctcccggtgc
     4981 agcaccagcg gcgtcgggat gcggcggaag aggtcctggt agcgcgtctc cgtcgaccac
     5041 agcgcctggc gcgccttcac gtcctgcgtg atgtcgcgca gcacgcccca gtagcccgcg
     5101 aacttgccgg cctcgttggt gcgcggctcg gcgctgagcg tgaagtggcg cacgcggccc
     5161 gccacgtccg accagcgcag aggcacgtcg cgcatcggca ggcgggcggc gagctcggag
     5221 cggaaggtct cgagcacctc gccgtcgaag gcgacgccgc gcgtctccca cagcagcttg
     5281 cccatgccgg cgtcgacgcc gtggcggcgc aggccgacga agttgcgcag ctcctccttc
     5341 gcggagaacc gcaccagccg cccgctgccg tccatctccc agtacgcgtc ggcggcgacg
     5401 gcgagcaggc ccaggaagcg gcgctcgcgc tcgtcggccg agcgcagcgt gcgatcgagc
     5461 atgcgcccca gcagctcgcc gccgcccagc gcgatcccaa gggccagcgc atgcgtgagc
     5521 agcggtcgcg ccagcacgaa caccggcatg ccgggtgcga ggccgggtgc cgtgatccag
     5581 tccagggccg tggcgaccgc cagcagcacc agcatcccga cgccgatcgc cgcggcgagc
     5641 agcccgcgcc tcagcccgcc gacgacggtg aacaggcaca gcagcacgcc cacgacggcc
     5701 agcgcgggcg acatcagccc ctcgccggcg accgccgcgg cgacgcccga caacccgatc
     5761 gccgtgccga tgacgtaggg cagcgcgcgc acctgggtgc cgatgccgcc gtcgcggcgc
     5821 agcgcgaacg cgacgcccgc gaggccgacg aacgcggtcg cgagccacag ccgcgccgcc
     5881 cagtccagcg gtgcgccggc gaccaggaac atgagcgcgg acgccgacgc cacgccggcc
     5941 gtcgccgcca gcaggtggcg ggtcagcgct tggcgcaggc gttgtcgtgc ctctttgttc
     6001 ggcgtagctt cagccatcga cgtcctgatc ggcgtccgct cccggcacgc cccccatgac
     6061 ccgcaagtgt gacgcttttt tcccgccgcc ggcacgggac gaatgcacaa accg
//
LOCUS       DASVLN010000433         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_452935, whole genome shotgun sequence.
ACCESSION   DASVLN010000433 DASVLN010000000
VERSION     DASVLN010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452935"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VF453_10635"
     CDS             <1..566
                     /locus_tag="VF453_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688157.1"
                     /translation="MTIGEGEGGRDKTAPRPLQDDAVAAAASAGLAFEGQTGLSRALY
                     ALLGEAVVRGARRICWCDLDFAAWPLGESDWIALLTRWARAGGRELVMVAADYRAVER
                     LHPRFVAWRRDWSHVIQCLVPDESRQSDPPTLWIDTLDQALRVFDREHWRGRAGFDRV
                     DCQRAREDFDAISQRATPGFSAVVLGL"
     gene            713..979
                     /locus_tag="VF453_10640"
     CDS             713..979
                     /locus_tag="VF453_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688158.1"
                     /translation="MNKSLLLASLVAVAALTACSKSADTSSTTAPSADSASAAASSSA
                     MAASSADTSASAAMSAASAASDAASSAADASANAAMAAASAASK"
     gene            complement(1224..2474)
                     /gene="bamC"
                     /locus_tag="VF453_10645"
     CDS             complement(1224..2474)
                     /gene="bamC"
                     /locus_tag="VF453_10645"
                     /inference="COORDINATES: protein motif:HMM:NF018509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEX7688159.1"
                     /translation="MTTSLPTRLLQPTPLRVATVTLCLMLGGCTLTGDWFGDHADYKA
                     GSKRTQPLEVPPDLTQLQRDSRYLPQGGVVAASDLTKAGTTPSAASPANAASVPTDGS
                     VAMNAVGDLHLEHAGGQRWLVTKESPEAVLPRVRAFWLSQGFTLKVDDPKSGILETDW
                     LENKTPLPGDTIHKLIGHVIDGLYDSGLRDRFRTRLERTPDNGTEIYVTHFGATEEVT
                     GFQKSEVQWVPRPNDPGLEAQEMSKLMFALGGVSDVNLAAAAPEKAGEDKPEARVIPS
                     AATQPASAGGSGPNGAMRARLVTGQPEAAMSLDDNFDRSWRRVGLALDHGGFTVEDRD
                     RAQGLYYVRYTDAKAAAEDEPGFFSKFTSWFTKSGPSPVGKYRISIKPSGDTCMVTVL
                     NDQGQPDNGDNAKRIVSLLVDELK"
BASE COUNT          348 a          899 c          920 g          434 t
ORIGIN      
        1 ccatgacgat cggtgaaggc gagggcgggc gcgacaagac ggcgccgcgg ccgctgcaag
       61 acgatgccgt cgcggctgcc gcctccgccg gcctggcgtt cgagggccag accgggctct
      121 cgcgggcgct gtacgcgctg ctcggcgagg ccgtcgtccg cggcgcgcgc cgcatctgct
      181 ggtgcgacct cgacttcgcc gcgtggccgc tcggcgagtc cgactggatc gccctgctga
      241 cgcgctgggc gcgcgcgggc gggcgcgagc tcgtgatggt cgccgccgac taccgcgccg
      301 tcgagcggct gcatccgcgc ttcgtggcct ggcggcgcga ctggtcgcac gtcatccaat
      361 gcctcgtgcc cgacgagagc cgccagtccg atccgccgac gctgtggatc gacacgctcg
      421 accaggcgct tcgcgtcttc gatcgggaac actggcgtgg ccgcgcggga ttcgaccgcg
      481 tcgactgcca gcgtgcgcgc gaagactttg acgccatttc gcaacgtgca acgccgggtt
      541 tttcggccgt agtcttggga ctctgaaaaa agttccgtgc ggatgggtat aatggcgggc
      601 taggcacagg aaatgctcga gcttgacctg ttgtcctttg agaggagcac ctgctgtgag
      661 ggcgggtgtc cgaaaatcac cggttcgaac ccaaatttct tcgaggaatt tcatgaacaa
      721 gtcgctcctg ttggcttccc tggtcgccgt cgctgccctg accgcctgca gcaagtccgc
      781 cgacacgtcg tcgacgaccg ctccgtcggc cgactccgcg tccgccgccg cctcgtcgtc
      841 ggccatggcc gcctcgtccg ctgacacgtc ggcttccgcc gcgatgagcg ccgcttcggc
      901 cgccagcgac gccgcctcgt cggccgccga cgcgtcggcc aacgccgcga tggccgccgc
      961 ttcggccgcc agcaagtaat tttccgccgc gccctgaagg gcgccgcagg aagattgaag
     1021 agccgccttc gggcggcttt tttgcgtccg cggcttcgcc gcaattgcat gacgacgagc
     1081 ggacaattgc cgtccgcttt gctcgtcgcg tctgcgcagc catctcgagc cattgtcgaa
     1141 tcggacagct tgccgcaagc cggaaaagga aaagccgccc gcgggcggct tcttcgcaat
     1201 gcgcgggacg gcgcccgaga cactcacttc aattcgtcga cgagcagcga gacgatgcgc
     1261 ttggcgttgt cgccgttgtc cggctggccc tggtcgttga gcaccgtgac catgcaggtg
     1321 tcgcccgacg gcttgatcga gatgcggtac ttgcccaccg gcgacgggcc gctcttggtg
     1381 aaccaggacg tgaacttcga gaagaagccc ggctcgtcct cggccgctgc cttcgcgtcg
     1441 gtgtagcgca cgtagtacag gccctgcgcg cgatcgcgat cctcgacggt gaagccgccg
     1501 tggtccagcg cgaggccgac gcggcgccac gaccggtcga agttgtcgtc cagggacatg
     1561 gccgcctccg gctggcccgt gaccaggcgc gcacgcatcg cgccgttcgg cccgctgccg
     1621 ccggcgctgg ccggctgcgt cgcggcgctc gggatcacgc gggcctcggg cttgtcctcg
     1681 ccggccttct cgggcgccgc cgcggcgaga ttcacgtcgc tcacgccgcc gagcgcgaac
     1741 atgagcttgc tcatctcctg cgcttccagg cccgggtcgt tcgggcgcgg cacccactgc
     1801 acctcgctct tctggaagcc ggtgacctcc tcggtcgcgc cgaagtgggt gacgtagatc
     1861 tcggtgccgt tgtccggcgt gcgctccagg cgcgtgcgga agcggtcgcg caggccggag
     1921 tcgtacaggc cgtcgatcac gtggccgatc agcttgtgga tggtgtcgcc cggcagcggc
     1981 gtcttgttct cgagccagtc ggtctcgagg atgccgctct tcgggtcgtc gaccttcaac
     2041 gtgaagccct gcgagagcca gaacgcgcgc acccgcggca gcacggcctc gggcgactcc
     2101 ttcgtcacga gccagcgctg gccgccggcg tgctccaggt gcaggtcgcc gacggcgttc
     2161 atcgcgacgc tgccgtcggt cgggacgctc gccgcgttcg ccggcgacgc ggcggacggc
     2221 gtcgtgccgg ccttggtcag gtcggacgca gcgacgacgc cgccctgagg caggtagcgc
     2281 gagtcgcgct gcagctgcgt gaggtcgggc gggacttcca gtggctgcgt gcgcttggag
     2341 ccggccttgt agtccgcgtg atcaccgaac cagtcgcccg tgagcgtgca cccgccgagc
     2401 atgagacaca acgtcacggt ggcgacgcgc aggggcgtgg gttggagcag gcgcgtcggg
     2461 agggaagtgg tcatgggtat gaggttcgag atcgggcccg gggttccggg ccggggattg
     2521 tcgccgaagc gcggccgcgc tcaggcgagg ccgcagtccg ccagcgccgc gtccaccagc
     2581 gcccggccgg cgggcgtcgc c
//
LOCUS       DASVLN010000434         5734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_343726, whole genome shotgun sequence.
ACCESSION   DASVLN010000434 DASVLN010000000
VERSION     DASVLN010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343726"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1717
                     /locus_tag="VF453_10650"
     CDS             <1..1717
                     /locus_tag="VF453_10650"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX7688160.1"
                     /translation="GEVVLDYRFEGQGMTGTASTRYDSATGDFVDSSVVGPTSGANGF
                     DGRQAWQRDASGAVTPQDGGDKRQLAVNEAYRDANAWWMPDHGGARIEALPGRTEGGH
                     AYDVLSVTPAGGKAFEAWFDARTHLLARTIEPQGFLVATTRYDDWREVGGWRVPGRVL
                     VDLGFGADQLQTMTLRKGDVVAARPAASYAIPRSTLADASIRNAQGRTTVPFKLINNH
                     VYAEVKVNGKGPFLFIFDTGGHDLLDPAAAKALKVASEGKAAGGGAGEGSVDVSFASG
                     VRFEIGDLEMHDQAISVLPIMSPAVEGIQEQGMMGFELFRRFVTTIDYGAQTLTFTDP
                     ARFRPGPELGTPVPFVFYDHLPQVRGTFEGAPGLFDIDTGSRAEITITKPFADANHLR
                     ESHPRGVVAVDGWGVGGRSVSYVARAKQLTLGPVEVDGLVAGFATQDKGAFSDPNFQG
                     NVGTRLLKRFVVTFDYGHQVMYLKPRPAPVPDIDSFDRSGLWINAAPAGYEIVDVAKG
                     SAGAEAGLVVGDRITRIDGAAPGALSLSDVRARLRDPKVDHVELVVQHAGAERTLTLR
                     LRDQV"
     gene            complement(1801..2064)
                     /locus_tag="VF453_10655"
     CDS             complement(1801..2064)
                     /locus_tag="VF453_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688161.1"
                     /translation="MQHLPHEPAARRAAIDRAIEWETLPSLAYLLGRDRDTAHNEPVW
                     ADTMPSEFDLDARAPSEDFIEPLPGLEIREVHEPDIFRLFFAA"
     gene            complement(2350..3141)
                     /locus_tag="VF453_10660"
     CDS             complement(2350..3141)
                     /locus_tag="VF453_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688162.1"
                     /translation="MKLPRPRLRPSSPALFTLLLLAGACLATSARADGLADLRAALAR
                     LSGHAPLKATLEVRGWRRSGEGADLQERQASVTLGLEDGPRGLQPVYGRELFARLDAE
                     ARAAIKDKKAPKPVSEVLEHTNVEELRLLASAAPAIARDVDESAFKGESADTLDGRPV
                     RKLVFERGIDSLADGDRKYAREFESVLTLWIAPDGTPLASRRHLQLAGRAFVVVTFQQ
                     RDDDDRSYAVLGDRLVTVRHDEKGHAQGAGENQDYRIERSLQVLP"
     gene            3380..5443
                     /locus_tag="VF453_10665"
     CDS             3380..5443
                     /locus_tag="VF453_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEX7688163.1"
                     /translation="MKTIPAGKTARPRAILGVVASAALTLLSACGGSHDDSAPKPAQG
                     TTAQVTLLETTDLHQNVLSYNYYTLAEDDSVGLERTATLIKQARAKYANTMLFDAGDI
                     IQGTVLGDYQALVNKVPCTDELAIFKAMDAIGYDAGTIGNHEFNYGLPYLAQVTGRTF
                     NVKNMPAVASQTKCKGPAYPQVLANVVSTTDNKPIFDPYVILDRQVTATTPDGKTVTA
                     TIKVGVIGFTPPQIMAWDKGNLDGNVTTVGLKEAAEQYVPEMKAKGADLIVAVSHGGP
                     DNAAYSPTMENGNLYLAQVPGIDVLLQGHMHQVFPNANSTVAAFNIAGVDKTAGTVAG
                     VPSVMANFWGKNLGVVNLTLTYGANGWTVDKTKSAASTMSTQNADKSYVAADPSIAPL
                     VKTEHDGTVAYVKTPIGDSDFRMSTYFADVGDVTAIEPVNQAQASYVKAYVQANLPQY
                     KSLPVLSVSAPFKSGFAGGSDYTDVASGSVAIFNAADLYLYANTIQAVLVTGADIKAW
                     LETAAQRFNQIDPTKTTPQALVSSFPGYNFDVMDGDGSIKYEIDVTQPAGSRIKNLTY
                     NGNAIDPAAQFIVATNNYRASGGGNFPGLNGSKTIYQSPDANRDVLIAYVKAQKTLAK
                     TVNGSDRSWHFTPVTTAGPVTFTSKPGNAALAAADGIPGVTQLQADDGSGKGLAVYQI
                     DLSAQ"
     gene            5440..>5734
                     /locus_tag="VF453_10670"
     CDS             5440..>5734
                     /locus_tag="VF453_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688164.1"
                     /translation="MKRSAAVLAAAGAIAGAGALAAATASWWRAPDAAQAQAWLKAAA
                     DAHDARAEAALARWSERGDAQARHAYAMLLAHRPDAGSQRALDTLLADGAAAGD"
BASE COUNT          925 a         2099 c         1983 g          727 t
ORIGIN      
        1 cggcgaagtg gtgctggact accgcttcga gggccagggc atgacgggca ccgcgtccac
       61 ccgctacgac agcgccaccg gcgacttcgt cgacagcagc gtcgtcggcc cgaccagcgg
      121 cgccaacggc ttcgacggcc ggcaggcctg gcagcgcgac gcgagcggcg ccgtgacgcc
      181 gcaggacggc ggcgacaagc gccagctcgc ggtcaacgag gcctaccgcg acgccaacgc
      241 ctggtggatg cccgaccacg gcggtgcaag gatcgaggcc ctgcccgggc gcacggaagg
      301 cggccacgcc tacgacgtgc tgtccgtgac ccctgcgggc ggcaaggcct tcgaggcctg
      361 gttcgacgcc cgcacccacc tgctcgcccg cacgatcgag ccccagggct tcctcgtcgc
      421 gaccacgcgc tacgacgact ggcgcgaggt cggcggctgg cgcgtccccg ggcgcgtgct
      481 cgtcgacctc ggcttcggcg cggaccagct gcagacgatg acgctgcgaa agggcgacgt
      541 cgtcgccgcc aggcccgccg ccagctacgc gatcccgcgg tcgacgctcg ccgacgcctc
      601 gatccgcaac gcccagggcc gcacgacggt gccgttcaag ctgatcaaca accacgtcta
      661 cgccgaggtg aaggtcaacg gcaaggggcc gttcctgttc atcttcgaca ccggcggcca
      721 cgacctgctc gaccccgcgg cggcgaaggc gctgaaggtc gcctccgagg gcaaggcggc
      781 gggcggcggc gcgggcgagg gctcggtgga cgtgagcttc gcgagcggcg tgcgcttcga
      841 gatcggcgac ctcgagatgc acgaccaggc gatctcggtg ctgccgatca tgtcccccgc
      901 cgtcgagggc atccaggagc agggcatgat gggcttcgag ctgttccgcc gcttcgtcac
      961 gacgatcgac tacggcgcgc agacgctcac gttcaccgac cccgcgcgct tcaggcccgg
     1021 cccggagctc ggcacgccgg tccccttcgt gttctacgac cacctgccgc aggtgcgcgg
     1081 cacgttcgag ggcgcgcccg gcctgttcga catcgacacc ggctcgcgcg ccgagatcac
     1141 gatcaccaag cccttcgccg acgccaacca cctgcgcgag agccatccgc ggggcgtcgt
     1201 cgcggtcgac ggctggggcg tcggcgggcg ctcggtgtcg tacgtcgcgc gcgcgaagca
     1261 gctcacgctg gggccggtcg aggtcgatgg cctcgtcgcc ggcttcgcga cgcaggacaa
     1321 gggcgcgttc tccgacccga acttccaggg caacgtcggc acgcggctgc tcaagcgctt
     1381 cgtcgtcacc ttcgactacg gccaccaggt gatgtacctg aagccgcgcc ccgcgccggt
     1441 gcccgacatc gactccttcg accgctcggg cctgtggatc aacgccgcgc ccgcgggcta
     1501 cgagatcgtc gacgtcgcca agggcagcgc cggcgccgag gcgggactcg tggtcggcga
     1561 ccgcatcacg cgcatcgacg gcgccgcgcc gggcgcgctg tcgctgtcgg atgtccgggc
     1621 gcggctgcgc gacccgaagg tggatcatgt cgagctggtc gtccagcacg cgggcgccga
     1681 gcgcacgctg accctgcggc tgcgcgacca ggtctgaggt ccggagacgc cgacgccgcc
     1741 ccggagcccc ggggcggcgt cggcgatggg aacacgggtc gcgcggaagc gacccgggga
     1801 tcaggcggcg aagaacaggc ggaagatgtc cggctcgtgc acctcgcgga tctcgaggcc
     1861 cggcagcggc tcgatgaagt cctccgaagg cgcgcgggcg tcgaggtcga actccgaggg
     1921 catcgtgtcg gcccagacgg gctcgttgtg cgcggtgtcg cggtcccgcc cgagcaggta
     1981 cgcgagcgag ggcagcgtct cccactcgat ggcgcggtcg atggcggcac ggcgagcggc
     2041 gggttcgtgc gggaggtgtt gcatgaaggt tctccaacga tgttgcttac gttactcttc
     2101 gtcacacgct cccgaaaact tgaataaggc cgagtagacc gaccagttga aatggccccc
     2161 gatcgccggc cagatgcgca cccaggccgc ggacgtgtcg gatgtcacgc tgcgccggcc
     2221 cggagccgcg gcccgcccgg gcgcgtcaga accccggctg cggatcgggc gccggcgtgg
     2281 cccggacgac gaggctccac gccagcgccg cgagcagcgc gccgatacgg gacgcggtac
     2341 gccgccagct cacggcagga cctgcaggct gcgctcgatg cggtagtcct ggttctcgcc
     2401 ggcaccctgc gcgtggccct tctcgtcgtg gcgcacggtc acgaggcggt cgccgagcac
     2461 ggcgtagctg cggtcgtcgt cgtcgcgctg ctggaaggtg acgacgacga acgcgcgtcc
     2521 cgccagctgc aggtggcggc ggctcgcgag cggcgtgccg tccggcgcga tccagagcgt
     2581 gagcaccgac tcgaactcgc gcgcgtactt gcggtcgccg tccgccaggc tgtcgatgcc
     2641 gcgctcgaac accagcttgc gcaccggcct gccgtccagc gtgtcggcgg actcgccctt
     2701 gaacgccgac tcgtcgacgt cgcgcgcgat cgcgggcgcg gccgaggcga gcaggcgcag
     2761 ctcctcgacg ttcgtgtgct ccagcacctc ggagaccggc ttgggcgcct tcttgtcctt
     2821 gatcgcggcg cgtgcctcgg cgtccaggcg cgcgaacagc tcgcgcccgt acaccggctg
     2881 caggccacgc gggccatcct ccaggcccag cgtgacgctg gcctggcgtt cctgcaggtc
     2941 ggcgccctcg ccgctgcggc gccagccgcg cacctccagc gtcgccttca gcggtgcatg
     3001 ccccgacagg cgggcgagcg ccgcgcgaag gtcggcaagg ccgtccgccc gcgccgacgt
     3061 cgcgaggcac gcgccggcca gcagcaggag cgtgaagagg gcgggcgaag acgggcgcag
     3121 gcgcggacgg ggcagcttca tcgacggcag atccaggcag gaaaggcctg cagtgtaggc
     3181 agccgacacg gcggccggcg accgcggcgc gacgaactgc gccgcgtgtc ggacgaaccg
     3241 tcgaggggcc acgggacagg cctggccgga gcggccgccc gcacgtggaa cggcgagccg
     3301 ccgcgatcct gtcacggccg cgtcacggcg ggtgcctaac gtgccggctt ttcgtctttt
     3361 cgtcggatcc gacaccatca tgaaaaccat tcctgcaggg aagaccgcgc gcccccgggc
     3421 gatcctcggc gtcgtcgcat cggccgccct caccctcctc tcggcctgcg gcggctcgca
     3481 cgacgactcc gcgccgaagc ccgcgcaggg cacgacggcg caggtcacgc tgctcgagac
     3541 gaccgacctg caccagaacg tcctgagcta caactactac acgctcgcag aggacgactc
     3601 ggtcggcctg gagcgcaccg ccacgctgat caagcaggcg cgcgcgaagt acgccaacac
     3661 gatgctgttc gacgccggcg acatcatcca gggcaccgtc ctgggcgact accaggcgct
     3721 cgtgaacaag gtgccctgca ccgacgagct cgcgatcttc aaggcgatgg acgcgatcgg
     3781 ctacgacgcc ggcaccatcg gcaaccacga gttcaactac ggcctgccct acctcgcgca
     3841 ggtcaccggc aggacgttca acgtcaagaa catgccggcc gtcgccagcc agacgaagtg
     3901 caagggcccg gcctatccgc aggtgctcgc caacgtcgtc agcacgaccg acaacaagcc
     3961 catcttcgac ccctacgtga tcctcgaccg ccaggtcacc gcgaccacgc ccgacggcaa
     4021 gaccgtgacg gcgacgatca aggtcggcgt gatcggcttc acgccgccgc agatcatggc
     4081 ctgggacaag ggcaacctcg acggcaacgt caccaccgtc ggcctgaagg aggccgccga
     4141 gcagtacgtg cccgagatga aggccaaggg cgccgacctg atcgtcgcgg tctcgcacgg
     4201 cggcccggac aacgcggcct actcgccgac gatggagaac ggcaacctct acctcgcgca
     4261 ggtgccgggc atcgacgtgc tgctgcaggg ccacatgcac caggtcttcc cgaacgcgaa
     4321 ctccaccgtc gccgcgttca acatcgccgg cgtcgacaag accgccggca cggtggcggg
     4381 cgtgccgtcg gtgatggcca acttctgggg caagaacctg ggcgtggtca acctcacgct
     4441 gacctacggc gccaacggct ggacggtcga caagaccaag tccgcggcct cgacgatgtc
     4501 cacgcagaat gccgacaaga gctacgtcgc ggccgacccg tcgatcgcgc cgctcgtcaa
     4561 gaccgagcac gacggcaccg tcgcctacgt gaagacgccg atcggcgact cggacttccg
     4621 catgagcacc tacttcgccg acgtcggcga cgtgaccgcg atcgagccgg tgaaccaggc
     4681 ccaggcctcg tacgtcaagg cgtatgtcca ggccaacctg ccgcagtaca agtcgctgcc
     4741 ggtgctgtcg gtgagcgcgc cgttcaagag cggcttcgcc ggcggctcgg actacaccga
     4801 cgtcgccagc ggcagcgtcg cgatcttcaa cgcggccgac ctctacctgt acgccaacac
     4861 gatccaggcg gtgctcgtga cgggcgccga catcaaggcc tggctcgaga ccgcggccca
     4921 gcgcttcaac cagatcgacc cgacgaagac gacgccgcag gcgctcgtga gctccttccc
     4981 gggctacaac ttcgacgtca tggacggcga cggctcgatc aagtacgaga tcgacgtgac
     5041 ccagccggcc ggcagccgca tcaagaacct gacctacaac ggcaacgcga tcgacccggc
     5101 cgcgcagttc atcgtcgcga cgaacaacta ccgcgcgagc ggcggcggca acttccccgg
     5161 cctgaacggc agcaagacga tctaccagag cccggacgcg aaccgcgacg tgctgatcgc
     5221 gtacgtgaag gcccagaaga cgctggccaa gacggtcaac ggcagcgacc gcagctggca
     5281 cttcacgccg gtgacgacgg ccggcccggt gaccttcacg tccaagcccg gcaacgccgc
     5341 gctcgcggcg gccgacggca tcccgggcgt cacccagctg caggcggacg acggctccgg
     5401 caagggcctg gcggtctacc agatcgacct gagcgcccag tgaagcgatc cgccgccgtc
     5461 ctcgccgccg caggcgccat cgcgggcgcc ggcgcgctcg ccgcggcgac ggcctcgtgg
     5521 tggcgcgcgc ccgacgccgc gcaggcgcag gcgtggctga aggcggccgc ggacgcgcat
     5581 gacgcgcgcg ccgaggccgc cctcgcccgc tggtcggaac gcggcgacgc ccaggcgcgg
     5641 catgcgtatg cgatgctgct cgcgcatcgg ccggacgcgg gctcgcagcg tgcgctcgac
     5701 acgctgctcg ccgacggcgc ggccgccggc gatg
//
LOCUS       DASVLN010000435         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_453000, whole genome shotgun sequence.
ACCESSION   DASVLN010000435 DASVLN010000000
VERSION     DASVLN010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453000"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(582..1154)
                     /locus_tag="VF453_10675"
     CDS             complement(582..1154)
                     /locus_tag="VF453_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7688165.1"
                     /translation="MPLDYRSAFEFSPVAVALSRERVIVDCNLKFLAMFRIQREHVIG
                     RSFEVIYPSHEDFERSGRRITIAVEASQDGSYADERVMRRPCDGSLFWCHVSGYALDR
                     ARPHAAGIWSLEDISASRPVAVELTPREREIGVLLVEGLTSKVVAKRLGLSYRTVEIY
                     RSRLMRKHGVDTATGLVRKLMGDALAGPAS"
     gene            complement(1260..2108)
                     /locus_tag="VF453_10680"
     CDS             complement(1260..2108)
                     /locus_tag="VF453_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7688166.1"
                     /translation="MIETLEPACAPDDGRVGGASATLVPAVVRALALLERVARQRQPM
                     SASQLATELALPRSSVHGLCHTLLHHGYLRRQGDGGFAIGASVMGLAEAFIADIDVAR
                     EFAGVWQEVGVATDEAVILSVLNGIDSIYVAVRNSPRPLGVAFNVGVRIPAHLSGSGK
                     AMLAFQPRETLVRLFDGVALGTFTGRGPANTAALFDELALIRDRGYSLDDEGVRAGVL
                     AFGAPVFDAAGRAVAGVAMCINKATLDEGALERHRDTLLDIARRLTRRLGGTAASGVA
                     RAARSS"
     gene            complement(2430..2517)
                     /locus_tag="VF453_10685"
     tRNA            complement(2430..2517)
                     /locus_tag="VF453_10685"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2481..2483),aa:Ser,seq:tga)
BASE COUNT          422 a          952 c          978 g          391 t
ORIGIN      
        1 tcgaatatct cgtcttcgtc accaaccaat acggcgacca aaaagtcagc gtgttcggtt
       61 tcggccacaa aaaatgatcc gccgtgcaaa ccccgtcgcc aggtgaatta gccgatgttc
      121 caggccctcg cgtggagcgt gaggctggaa cgacggcgac gcccgccgtc cgggcgatgc
      181 ggcggctggc cgcacttcgg ggcagccgcc ggtgcgggcg ctcacccgag cgcgtctgcg
      241 acagtgaggt cgcgtgcgcg cgggagtctc gcgtcgcgtg caatccgagc gggcaggccg
      301 cgtgcgccag cgcgggcgcg tcgcgggccc gtccttgcac gatccctccg catgcgcgcg
      361 atcgaggtgg cgtgcgcgcc gggcacggcg tgccctcgtc ccaacttcat gcctgtctcc
      421 tgtcgttgtc gtcgcttcgg tcctcgcggc gagccacggg acctcgccgc aatttagggc
      481 gaagggcgac cgctcgtcag tcgtaggaac acattggggt tttcgccagc gccgacgcgc
      541 gccgagggcc acgctcccgc ggtgcgggcg cggccggagg atcaggaggc cgggccggcc
      601 aatgcatcgc ccatgagctt gcgcaccagg ccggtcgccg tgtcgacgcc gtgcttgcgc
      661 atgagccggc tgcgatagat ctccacggtg cggtagctga gcccgagccg cttggcgacg
      721 accttgctgg tcaagccctc gacgagcagc acgccgatct cgcgctcgcg cggcgtcagt
      781 tccacggcga ccgggcgcga ggcggagatg tcctccagcg accagatgcc cgccgcatgc
      841 ggacgcgcgc gatcgagcgc gtagcccgac acgtggcacc agaacaggct tccgtcgcag
      901 ggccggcgca tcacgcgctc gtccgcgtag ctgccgtcct gcgaagcctc caccgcgatg
      961 gtgatgcgcc ggccgctgcg ctcgaagtcc tcgtgcgacg gatagatcac ttcgaacgac
     1021 cgcccgatca cgtgctcgcg ctggatgcgg aacatggcga ggaacttcag gttgcagtcg
     1081 acgatgaccc gctcgcgcga cagcgccacg gccaccggcg agaactcgaa ggcgctgcgg
     1141 tagtccagcg gcaggtcgtc gcgcttggcg ggcaggggct gcgtgtcgag ggtggccatg
     1201 gcgatcttca ggggcggtgt gtcgcggcgg acgacgggga cgccggtgtc ggcgggagat
     1261 cagctcgaac gtgcggcgcg cgcgacgccg ctggccgccg tcccgccgag gcggcgcgtg
     1321 agccgccggg cgatgtccag cagcgtgtcg cggtggcgct cgagggcgcc ctcgtccagc
     1381 gtggccttgt tgatgcacat cgccacgccc gcgacggcgc gcccggccgc gtcgaagacc
     1441 ggggctccga aggcgagcac gcccgcgcgc acgccttcgt cgtcgaggct gtagccgcgg
     1501 tcgcggatca gcgcgagctc gtcgaacagc gctgccgtgt tcgcgggtcc gcgaccggtg
     1561 aacgtcccga gggccacgcc gtcgaagagc cgcacgagcg tctcgcgcgg ctggaaggcg
     1621 agcatggcct tgccgctgcc cgaaaggtgg gccgggatcc gcactccgac gttgaaggcc
     1681 acgccaagcg ggcgcggact gtttcgcacg gccacgtaga tcgagtcgat gccgttgagc
     1741 acggacagga tcaccgcctc gtccgtcgcc acgcccacct cctgccacac gcccgcgaat
     1801 tcgcgagcca cgtcgatgtc cgcgatgaat gcttcggcca gccccatcac gctggccccg
     1861 atcgcgaagc cgccgtctcc ctgacggcgc aggtagccgt gatgcagcag cgtgtggcac
     1921 aggccgtgca cggaactgcg aggcagcgcg agctcggtcg cgagctggct ggccgacatc
     1981 ggctggcgct ggcgcgccac ccgctccagc agcgccaggg cccgcaccac ggcaggcacg
     2041 agcgtcgccg acgcgccgcc cacgcgaccg tcatccggcg cgcaagccgg ctccagcgtc
     2101 tcgatcacga ctgatcaccc tccgtcgtta ttcagccggc tgaacataat tcagccaata
     2161 gacgacagtc tacccggcga cgcaccagct tggtggacga gccggcaggg actcgggtaa
     2221 atgcggagtc cggatgtcac gcaatggggc gcggcgtgca attcggccgc cgatggagcg
     2281 gccagcgtgg cgggggctgc cgcgaggaca gggccaatgc gctgacgagg gcgtatgacg
     2341 ccggcgatcc ccggctcccg ccggcgccat cgcgcgcgcc ctcgcggcat cacgccgttc
     2401 gcaaccgcgg ggcgacgcac gcgccaggtt ggcggaggct gtgggattcg aacccacgga
     2461 cggttgcccg tcggcagttt tcaagactgc tggtttaaac cactcaccca aacctccggg
     2521 ccggtgcatt gtaggcgctg gtcgcagggc gggaacccgg acggcggaac gtgaaccaag
     2581 cgaatggatc aaccatggga gacaagccgg tgaagctttc cagccaggtg gccgcaggcc
     2641 acctggctgg aaagctaggt ggccgcaggc ctcgtcgtca tcggcctggg cgcgtcgaga
     2701 ctcgcgttcg gaggccctgc gtccgcgccg gcggtcggcg ctg
//
LOCUS       DASVLN010000436         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_507206, whole genome shotgun sequence.
ACCESSION   DASVLN010000436 DASVLN010000000
VERSION     DASVLN010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507206"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..2288
                     /gene="metG"
                     /locus_tag="VF453_10690"
     CDS             243..2288
                     /gene="metG"
                     /locus_tag="VF453_10690"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7688167.1"
                     /translation="MTRKLFVTTALPYANAPFHIGHMMEYIQADIWVRFQRMRGAEVH
                     FVCADDAHGAPIMIAAEKAGKTPQAFVADIAAGRARYLDGFHIAFDNWSSTDSPENHA
                     LAQEIYLALRKNELIDVKTIEQFFDPVKSMFLPDRFIKGECPKCGAKDQYGDSCEVCG
                     SVYSPTELRNPYSALSGATPVIKSSEHYFFRLSDPRCVEFLQKWTTDTGRLQPEVLNK
                     IREWFAKDDEGQGGLGDWDISRDAPYFGIEIPDAPGKYFYVWLDAPIGYLASLKNYFG
                     RTGRDFDAFMADPDVQQVHFIGKDITYFHTLFWPAMLHFSGRKVPDHVFVHGFITVNG
                     GEKMSKSRGTGISPLKYLELGLHPEWLRYYIAAKLNAHVEDVDFNPDDFKARVDADLV
                     GKYVNIASRAAGFLGKRFGGRLSGDLGVEGSALLETLRAQRGAIETLYEEREYGKALR
                     EVMALADKVNEYVDLHKPWELAKKAGADAALQDVCSTCIEAFRLLTIYLKPVLPALAA
                     QVEAFLQVAPLTFADVATPLGAHAIGAYPRLMERVDPKLLDALFEPPPAPVVVPGGEP
                     VAAEIKLDDFARVDLRVARIVNAEKVEGSDKLLRLTLDVGEGRHRNVFSGISAHFAPE
                     ALVGKLTVVVANLAPRKMKFGVSEGMVLAASHADEQVDGGLYLLEPGTGALPGMRLH"
     gene            2378..2581
                     /locus_tag="VF453_10695"
     CDS             2378..2581
                     /locus_tag="VF453_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEX7688168.1"
                     /translation="MPLFWKPYKSEITQFLDELKSKKPSLDHEQAEGRALLWDKKVDR
                     ELQAEFREARVAQQPYVYQTHAK"
BASE COUNT          443 a          969 c          896 g          369 t
ORIGIN      
        1 cggcggtggc gacggcggcg ggatcgggca tcggagaggg gcggggatcg gcaggcgccg
       61 tgggcgcgaa cccgaggaag gcttcgcatt cgaccaccga cgcgatcccg gggcgaggcg
      121 ctggggcttt ggggcctgtt cgcgaccctg ccgcgggcgc cggggctcgc ccttagaatc
      181 gcctgttccg cccagcacgg cccggccccc gggccgcgcg ggcgcagccc cgacgcgacg
      241 ccatgacccg caaactcttc gtcaccaccg ccctgcccta cgccaacgcg cccttccaca
      301 tcgggcacat gatggagtac atccaggccg acatctgggt gcggttccag cgcatgcgcg
      361 gcgccgaggt gcacttcgtc tgcgccgacg acgcgcacgg cgcgccgatc atgatcgccg
      421 ccgagaaggc cggcaagacg ccgcaggcct tcgtcgccga catcgccgcc ggccgcgcca
      481 ggtacctcga cggcttccac atcgcgttcg acaactggag ctccaccgac agccccgaga
      541 accacgcgct ggcgcaggag atctacctcg cgctgcgcaa gaacgagctg atcgacgtca
      601 agacgatcga gcagttcttc gacccggtca agtcgatgtt cctgcccgac cgcttcatca
      661 agggcgaatg cccgaagtgc ggcgcgaagg accagtacgg cgactcgtgc gaggtctgcg
      721 gctccgtcta cagcccgacg gagctgcgca acccgtactc ggcgctgtcc ggggcgacgc
      781 ccgtcatcaa gtcgagcgag cactacttct tccgcctgtc cgacccgcgc tgcgtcgagt
      841 tcctgcagaa gtggacgacg gacaccggcc gcctgcagcc cgaggtgctc aacaagatcc
      901 gcgagtggtt cgccaaggac gacgagggcc agggcgggct cggcgactgg gacatcagcc
      961 gcgacgcgcc ctacttcggc atcgagatcc ccgacgcccc gggcaagtac ttctacgtct
     1021 ggctggacgc gccgatcggc tacctggcct cgctgaagaa ctacttcggc cgcaccggcc
     1081 gcgacttcga cgccttcatg gccgaccccg acgtgcagca ggtgcacttc atcggcaagg
     1141 acatcaccta cttccacacg ctgttctggc cggcgatgct gcacttcagc ggccgcaagg
     1201 tgcccgacca cgtgttcgtg cacggcttca tcaccgtcaa cggcggcgag aagatgagca
     1261 agagccgggg caccggcatc tcgccgctga agtacctcga gctgggcctg catcccgagt
     1321 ggctgcgcta ctacatcgcg gccaagctca acgcgcacgt cgaggacgtc gacttcaatc
     1381 ccgacgactt caaggcccgc gtcgacgccg acctggtggg caagtacgtc aacatcgcca
     1441 gccgcgccgc cggcttcctg ggcaagcgct tcggcggccg gctgtcgggc gacctcggcg
     1501 tcgagggctc ggcgctgctg gagacgctgc gcgcccagcg cggcgccatc gagacgctct
     1561 acgaggaacg cgaatacggc aaggcgctgc gcgaggtgat ggccctggcc gacaaggtca
     1621 acgagtacgt cgacctgcac aagccctggg aactcgcgaa gaaggccggc gccgacgccg
     1681 cgctgcagga cgtctgcagc acctgcatcg aggcgttccg cctgctgacg atctacctga
     1741 agccggtgct gccggcgctg gccgcccagg tcgaggcctt cctgcaggtc gcgccgctga
     1801 ccttcgccga cgtcgccacg ccgctcggcg cgcacgcgat cggcgcctat ccgcgcctga
     1861 tggagcgcgt cgacccgaag ctgctggacg cgctgttcga gccgccgccc gcgccggtgg
     1921 tcgtgccggg cggcgagccg gtcgccgccg agatcaagct cgacgacttc gccagggtcg
     1981 acctgcgcgt ggcgcgcatc gtcaacgcgg agaaggtcga gggctccgac aagctgctgc
     2041 gcctgacgct ggacgtcggc gaagggcggc accgcaacgt gttctcgggc atctcggcgc
     2101 acttcgcgcc cgaggcgctg gtgggcaagc tgaccgtggt cgtcgccaac ctcgcgccgc
     2161 gcaagatgaa gttcggcgtg tcggagggca tggtgctcgc ggccagccat gcggacgagc
     2221 aggtcgacgg cggcctgtac ctgctggagc ccggcaccgg cgcgctgccg ggcatgcggc
     2281 tgcattgatc gcgcgcggcg cagccgtgcc gccatcgtct aaaatcccgc ctccgatcgt
     2341 gcacgcgccc gtgcatccga cgcagacaag aatcgccatg ccgctcttct ggaagcccta
     2401 caagtccgaa atcacgcagt tcctcgacga gctgaagagc aagaagccgt cgctcgacca
     2461 cgagcaggcc gaaggccgcg cgctgctctg ggacaagaag gtcgaccgcg agctccaggc
     2521 cgagttccgc gaggcacgcg tcgcccagca accctacgtc taccagacgc acgcgaagtg
     2581 aaccccgagg cgccggccgc cggcgacggc gccggtgctg ccgccggcgc cagcggcgat
     2641 gcatccccgc tgatcgccca ggccgccgag gccggcc
//
LOCUS       DASVLN010000437         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_183953, whole genome shotgun sequence.
ACCESSION   DASVLN010000437 DASVLN010000000
VERSION     DASVLN010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183953"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="VF453_10700"
     CDS             <1..1487
                     /locus_tag="VF453_10700"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX7688169.1"
                     /translation="LGVSQVAQDPAAATQALGLTTVVATVQADGARWQADAEVAGTRV
                     GELNGRWIVQSSSPAALPTPESPLQGQVHAHVADLNVWGAWLPPGWRLGGELRTDLAL
                     SGRWGAPHLTGDLAATKLAVRNALEGVYGHDGDVEIRLSGDQARIERFRLRGGNGELT
                     IAGDASLGASPAARLKLHAAQFQLLGRIDRKIVTSGDVDLALGADAIKVTGKLGIDEG
                     LIDISKGDAPTLDSDVQVLDGSADSSDDTPAAPAAPRTGRATTIALDLALGDQLHLRG
                     RGIDTFLRGDLQISAPNGLLAVRGTIRTNGGQYIAYGQKLQVTRGELVFTGIVNDPRL
                     DILATRPNLDVTVGVAITGTALAPHVKLVSEPEMSDADKLSWLMLGRAPDTLGGADTA
                     LLQQAAIALLAGEGEGPTDQILNRLGLTDFGVGQKTDANDVKQTVVTLGRQISRRVYI
                     GYERGVNATTGNWQLIYRIAQRLTVRAQSGEDNAADVIYTWRWN"
     gene            1633..2250
                     /locus_tag="VF453_10705"
     CDS             1633..2250
                     /locus_tag="VF453_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7688170.1"
                     /translation="MQPDHASPSHPPAPAAGPPSHDDAEDRRLVQRTCAREPAAFEAL
                     YRRYHPRLTRFLHRMLRPEHLIEEVLNDTLHVVWNQAERFNGTSKVSTWIFAIAYRKA
                     LKAIERHDEPLPDVPDDEQAAADAFDRPEARMSRAQARAAIDSALRTLSADQRAAVEL
                     TYFHGFSYPEIAQIVDCPTDTVKTRMFHARRRLRAAFAGGLDDWL"
     gene            2375..>2688
                     /locus_tag="VF453_10710"
     CDS             2375..>2688
                     /locus_tag="VF453_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7688171.1"
                     /translation="MTGQIVHLEPDPHVAVQQLLPWYLTDRLAPAERAQVDAHVANCA
                     ECRAELEAERRWQAGPVQALLQADSGTAHGDVEQGWGRMRALLGDGVAAGRDGDARPA
                     ATS"
BASE COUNT          392 a         1020 c          962 g          314 t
ORIGIN      
        1 acctgggcgt gtcgcaggtg gcgcaggatc ccgcggccgc gacgcaggcg ctcggcctga
       61 cgacggtcgt ggcgaccgtg caggccgacg gcgcgcgctg gcaggccgac gccgaggtcg
      121 cgggcacccg cgtgggcgag ctgaacgggc gctggatcgt gcaatcgtcc tccccggcgg
      181 ccctgccgac gcccgagtcg ccgctgcagg gccaggtgca tgcgcacgtg gcggacctca
      241 acgtctgggg cgcctggctg ccgcccggct ggcgcctggg cggcgagctg cgcaccgacc
      301 tggcgctgtc gggccgctgg ggcgcgccgc acctgaccgg cgacctcgcg gccacgaagc
      361 tggcggtgcg caacgcgctc gagggcgtgt acgggcacga cggcgacgtc gagatccgct
      421 tgtccggcga ccaggcgcgc atcgagcgct tccgcctgcg gggcggcaac ggcgagctca
      481 cgatcgccgg cgacgcgagc ctgggcgcct cgcccgcggc ccggctgaag ctgcacgcgg
      541 cgcagttcca gctgctcggg cgcatcgacc gcaagatcgt gacgagcggc gacgtcgacc
      601 tcgcgctcgg cgccgacgcg atcaaggtga cgggcaagct gggcatcgac gagggcctca
      661 tcgacatcag caagggcgac gcgccgacgc tggactccga cgtgcaggtg ctcgacggct
      721 cggccgactc gtccgacgac acccccgccg cgccggcggc gccgcgtacc ggccgcgcga
      781 cgacgatcgc gctggacctg gcgctcggcg accagttgca cctgcgcggg cgcggcatcg
      841 acaccttcct gcgcggcgac ctgcagatca gcgcgcccaa cggcctgctc gccgtgcgcg
      901 gcacgatccg caccaacggc gggcagtaca tcgcctacgg gcagaagctg caggtcacgc
      961 gcggcgagct cgtcttcacc ggcatcgtca acgacccgcg gctggacatc ctcgcgaccc
     1021 ggcccaacct cgacgtcacg gtgggcgtgg ccatcaccgg cacggcgctg gcgccgcacg
     1081 tgaagctggt gagcgagccg gagatgagcg acgccgacaa gctctcctgg ctgatgctgg
     1141 gccgggcgcc cgacacgctc ggcggcgccg acaccgcgct gctgcagcag gcggcgatcg
     1201 cgctgctcgc cggcgagggc gagggcccga ccgaccagat cctcaaccgc ctcggcctga
     1261 ccgacttcgg cgtcggccag aagaccgacg ccaacgacgt caagcagacc gtcgtgacgc
     1321 tggggcggca gatctccagg cgcgtctaca tcggctacga gcgcggcgtg aacgcgacca
     1381 ccggcaactg gcagctgatc taccgcatcg cgcagcgcct gacggtgcgc gcgcagtcgg
     1441 gcgaggacaa cgcggcggac gtgatctaca cctggcgctg gaactgagcg cgcgggtgct
     1501 gcctgcgggg tcgcgcgcgc atttcgggca ggaaatcacg gctttcgcgc ggggcggagt
     1561 cgggcgcggc gggccagaat tgaaccgggc cgctcgctgc cggcattcac ggtcgagacg
     1621 agcccgcccg ccatgcaacc cgatcacgcc tcgccctccc atccgcccgc cccggccgcc
     1681 ggcccgccgt cgcacgacga cgcggaggat cgacgcctcg tgcagcgcac ctgcgcgcgc
     1741 gagcccgcgg ccttcgaggc gctctaccgc cgctaccacc cgcggctcac gcgcttcctg
     1801 catcgcatgc tgcggcccga gcacctgatc gaggaggtgc tcaacgacac gctgcacgtc
     1861 gtctggaacc aggcggagcg cttcaacggc acgagcaagg tgtccacctg gatcttcgcg
     1921 atcgcctacc gcaaggcgct caaggcgatc gagcgccacg acgagcccct gcccgacgtg
     1981 cccgacgacg agcaggccgc cgccgacgcg ttcgaccgtc cggaggcgcg catgagccgg
     2041 gcccaggcgc gcgcggcgat cgacagcgcg ctgcgtaccc tgtccgccga ccagcgcgcg
     2101 gcggtcgagc tcacctattt ccacggcttc agctatcccg agatcgcgca gatcgtcgac
     2161 tgcccgacag acaccgtgaa gacccgcatg ttccacgcgc gccgacgcct gcgcgccgcg
     2221 ttcgcgggtg gactggacga ctggctgtga accgtcgcac catggacgcc cgcgtcccga
     2281 cccgcctttc cggcccgccg cgcccgagcc cgcgcacggc cgctcccgcc accccgggct
     2341 cgcaccgcag gcgcctgcgg cgaaagacca gctcatgacc ggccagatcg tccatctcga
     2401 acccgacccg cacgtcgcag tgcagcagtt gctgccgtgg tacctcaccg accggctcgc
     2461 gccggcagag cgcgcgcagg tcgatgccca cgtggccaac tgcgcggagt gccgggccga
     2521 actggaggcc gagcggcggt ggcaggccgg cccggtgcag gcgctgctgc aggcggactc
     2581 gggcacggcc cacggtgacg tcgagcaagg ctggggccgc atgcgtgcgc tgctgggcga
     2641 cggcgttgcc gccggccggg acggcgacgc gcggcccgcg gcgacatc
//
LOCUS       DASVLN010000438         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_184071, whole genome shotgun sequence.
ACCESSION   DASVLN010000438 DASVLN010000000
VERSION     DASVLN010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184071"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VF453_10715"
     CDS             <1..310
                     /locus_tag="VF453_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HEX7688172.1"
                     /translation="TGRGGRNVEFLLALALALDGRPGIHAIAGDTDGVDGAEEIAGAV
                     VAPDTLARAWALGLPPRASLDANDAHTFFRALGDSVVTGPTLTNVNDFRAILVMPPAG
                     "
     gene            complement(347..1681)
                     /locus_tag="VF453_10720"
     CDS             complement(347..1681)
                     /locus_tag="VF453_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688173.1"
                     /translation="MSHPKFMRRRFPMQSIVRRFLGTNGLRLGIFLGALLAAGTASAV
                     PSFARQTGQPCAACHVGGFGPQLTDFGIAFKLGAYTLAVPDRPAVPVAAMVMTGFTHT
                     KSAQDPAGLDGHRLGGAALKGNDNLTLDQVSLFLAGRLASKAGVFGQLTYDGVAQHAS
                     IDNVDVRVADTFTLPGDVALLAGVSVNNNPGVQDPFNTAPAWAFPYISSPVAPGPSAA
                     TILDEAWGQKATGVVAYGQVDGFWYGELGTYRMQTVSTQKFFGLGADDRDPGALRSPL
                     YARLAVRPSWNGGSLNAGVVLFRTGYQPDRGQPDTTHYTDVGVDASWREVFADASQAT
                     VLASVIHEAASGTPGMTEYTATGSYYLPSPWGGSWGANLQRFGVNASGLASRGSRLQL
                     DWTPTGTRPLRGVWDPSVRVGLQFTAYDELDAQRGHAAAVADSAYLFAWLAF"
     gene            2137..2445
                     /locus_tag="VF453_10725"
     CDS             2137..2445
                     /locus_tag="VF453_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEX7688174.1"
                     /translation="MRANRRLFLLTTALIGQAGLPGRGIAAPAALDPSDPQATILGYT
                     PDATKPAPSFATRHAAGAHCGNCALFQGKAGDAPGPCPLFGGKLVQGSGWCSSWVAKG
                     "
     gene            complement(2509..2973)
                     /locus_tag="VF453_10730"
     CDS             complement(2509..2973)
                     /locus_tag="VF453_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7688175.1"
                     /translation="MIRRPIVALCLGVLAMANAAETTPAQPAGRITGIGGVFFKAKDP
                     KALAAWYRDVLGLPVQAWGGAALRFDAPGHPPAAAWSTFPASTDYFAPSTSAFMIDYA
                     VDDLDALLARLHAKGVEVLKRDDSDDHGRFAWVLDPEGNKVELWEPKRPPAP"
     gene            3146..>3521
                     /locus_tag="VF453_10735"
     CDS             3146..>3521
                     /locus_tag="VF453_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7688176.1"
                     /translation="MENVPTAPPPARAPTPVDAWRRYDADEARLSAHVSERMLDLAGL
                     RAGARVLDIASGRGEPAVRAARRVAPDGVVVGTDRSDEMLAFARARAVAESVANLSLR
                     VADGETLAGIPDGAFDAALCRWG"
BASE COUNT          499 a         1271 c         1276 g          475 t
ORIGIN      
        1 cacgggccgc ggcggacgca acgtggagtt cctgctcgcg ctcgcgctcg ccctggacgg
       61 ccggcccggc atccacgcga tcgcgggcga caccgatggc gtcgacggcg ccgaggagat
      121 cgccggcgcc gtcgtcgccc ccgatacgct cgcgcgcgcc tgggcgctgg gcctgccgcc
      181 gcgcgcgagc ctcgacgcca acgacgccca caccttcttc cgcgcgctcg gcgactcggt
      241 cgtcacgggg ccgacgctga cgaacgtcaa cgacttccgc gcgatcctcg tgatgcctcc
      301 ggccggctga tcggcacgcg ccgcccgcgc tccggggacg tgccgatcag aaggcgagcc
      361 aggcgaacag gtaggcgctg tcggccacgg ccgcggcgtg gccgcgctgc gcgtcgagct
      421 cgtcgtaggc ggtgaactgc aggccgacgc gcacgctcgg atcccacacg ccgcgcagcg
      481 ggcgcgtgcc ggtcggcgtc cagtccagct gcaagcggct gccgcgcgat gccagccccg
      541 acgcgttcac gccgaagcgc tgcaggttcg cgccccacga gccgccccag ggcgacggca
      601 ggtagtacga gccggtcgcc gtgtactcgg tcatgcccgg cgtgcccgac gccgcctcgt
      661 ggatcacgct cgcgagcacg gtcgcctggc tcgcgtcggc gaagacctcg cgccagctcg
      721 cgtcgacgcc gacgtccgtg tagtgcgtcg tgtccggctg tccccggtcg ggctggtagc
      781 cggtgcgaaa gaggacgacg ccggcgttga gcgagccgcc gttccagctc ggtcgcaccg
      841 cgaggcgcgc gtacagcggc gagcgcagcg cgccgggatc gcggtcgtcg gcgccgaggc
      901 cgaagaactt ctgcgtcgac acggtctgca tgcggtaggt gcccagctcg ccgtaccaga
      961 agccgtcgac ctggccgtag gcgaccacgc ccgtcgcctt ctggccccag gcctcgtcga
     1021 ggatggtcgc ggccgacggc cccggcgcga caggcgacga gatgtagggg aaggcccagg
     1081 ccggcgccgt gttgaacgga tcctgcacgc ccgggttgtt gttgaccgac acgcccgcca
     1141 gcagcgcgac gtcgcccggc agcgtgaagg tgtcggccac gcgcacgtcg acgttgtcga
     1201 tcgacgcatg ctgcgcgacg ccgtcgtagg tgagctggcc gaacacgccg gccttggacg
     1261 cgaggcgtcc ggcgaggaac agcgacacct ggtccagcgt caggttgtcg ttgcccttga
     1321 gcgcggcgcc gcccaggcgg tggccgtcga ggccggcggg atcctgcgcc gacttggtgt
     1381 gggtgaatcc ggtcatcacc atcgccgcga ccggcaccgc gggccgatcc ggcacggcca
     1441 gggtgtaggc gcccagcttg aacgcgatgc cgaagtcggt cagttgcggc ccgaagccgc
     1501 cgacatggca ggccgcgcag ggctgcccgg tctggcgcgc gaagctcggg acggccgacg
     1561 cggtgccggc cgcgagcagg gcacccagga agattcctag gcgcaatccg ttcgtgccga
     1621 gaaatctcct aacgattgat tgcatcggaa atctccgacg catgaacttg ggatgggaca
     1681 tcacgaatct cctgagatgg gatgaagtac cggcgctcct gccgtgcagc cgcgggagcg
     1741 ccgccggtgt cagtgagtgc ccgcgtcgac gcgggacgaa tcggcccgta cctggcaggg
     1801 tatgctggcc gctccgccgt gacgcggcaa atccgtgcat tccctgttag ggtggacgca
     1861 cgggcttccg gttacgaacc gcgacagtcc acatgcgcga gcaatgcgcc atccagcatg
     1921 gcgtcgcacg aggaacgacg caggaatccc tggaaccggt catgtctagg catgttcaga
     1981 caggcgacag ctcgcgggcg tcgcgactta ccaacgcgag cgggcgaccg gggcgcagga
     2041 tcccggatcg acgatgcggg catcgacgag gcatcgacgg cgcgtgctaa cttgaaccga
     2101 ttcgaacccg cccggaatcc acgaggcaat cacatcatgc gagccaaccg tcgccttttc
     2161 ctcctgacca cggccttgat cggacaagcc ggcctgccgg gccgcggcat cgccgcgccc
     2221 gccgcgctcg atccgtccga cccgcaggcc acgatcctcg gctacacgcc cgatgcgacg
     2281 aagcctgcgc cgtcgttcgc gacccggcac gccgcgggcg cccattgcgg caactgcgcg
     2341 ctgttccagg gcaaggccgg cgacgcgccc gggccgtgcc cgctgttcgg cggcaagctg
     2401 gtgcagggca gcggctggtg cagcagctgg gtcgcgaaag gctgagtcca ggcctgcgcc
     2461 gcgcacggtt gcggtgctcc cgcccggccg cggcctcgac tcgttgcgtc agggggccgg
     2521 cgggcgcttc ggctcccaga gctcgacctt gttgccctcc ggatccagca cccatgcgaa
     2581 gcggccgtgg tcgtcgctgt cgtcgcgctt gagcacctcg acgcccttgg catgcagccg
     2641 cgcgagcagc gcgtccaggt cgtcgacggc gtagtcgatc atgaaggcgg aggtcgacgg
     2701 cgcgaagtag tccgtggacg cgggaaacgt gctccacgcc gccgccggcg gatgccccgg
     2761 agcgtcgaag cgcagcgccg cgccgcccca ggcctgcacc ggcaggccca gcacgtcgcg
     2821 gtaccaggcc gccagggcct tcggatcctt cgccttgaag aacacgccac cgatgccggt
     2881 gatgcgcccg gcgggctggg cgggggtggt ttcggctgca ttggccatcg cgagaactcc
     2941 caggcacagc gccacgatag gacgtcggat catcgccgtg ctccttgcag cgccggtcgc
     3001 cgctgcgtgc gttgaaggcc cgcgtgcgcg ggagcgagcc ggcatttgac cgcatccgca
     3061 tgcccaaggc cagcggctcg ggcctcgctt cagcttcgcc gcggcggcgg cgagcgccgg
     3121 agcggccgga cgtatgctcg cccgcatgga aaacgtcccg accgccccac cccctgcgcg
     3181 cgcgccgacc ccggtcgacg cctggcgccg ctacgacgcc gacgaggccc ggctgtcggc
     3241 gcacgtcagc gagcgcatgc tcgacctcgc aggcctgcgg gccggggcgc gcgtgctgga
     3301 catcgcctcc ggacgcggcg agccggccgt gcgcgcggcg cggcgcgtgg cgccggacgg
     3361 ggtggtggtc ggcaccgatc gctcggacga gatgctggcc ttcgcacgcg cgcgggcggt
     3421 cgccgagtcg gtggcgaacc tgtcgctgcg ggtcgccgac ggcgagacgc tcgccgggat
     3481 cccggacggc gccttcgacg ccgccctgtg ccgctggggg c
//
LOCUS       DASVLN010000439         4034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_397742, whole genome shotgun sequence.
ACCESSION   DASVLN010000439 DASVLN010000000
VERSION     DASVLN010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397742"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            630..1571
                     /locus_tag="VF453_10740"
     CDS             630..1571
                     /locus_tag="VF453_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7688177.1"
                     /translation="MKRHLLIGAALLWSAVAFAVPSRADVESAVKAGDYARAETMMRE
                     VVTADPQSARAHYYFAEVLAHEGKFDDAVGQARQARQLDPRIGFTDPAKFTQFEQLLR
                     REQSHAGTTTPAPGTLPAATDAVPAPAPAPVVRATSGMPGWVWVLGIAAVLFFVWRMA
                     TRRLASSPSMQPAGYGGAGFAQPGYGQPGYGQPGYGQPGYGQPGYGQPGYGPGYPQPG
                     AGGGMLRTGLAAAGGLAAGMALEHMLDERHERDRYVDDNLAGGNVGGGIPAPFDDDSS
                     RAATELENRPFDFGNDAGGGWDDGGGSGGGSSDDGGW"
     gene            complement(1653..1904)
                     /locus_tag="VF453_10745"
     CDS             complement(1653..1904)
                     /locus_tag="VF453_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688178.1"
                     /translation="MKPAALPLLVLLAAGLAAPGASAKGCLKGAVVGGVGGAVVKHPL
                     IGAAAGCAVGHHEAKKKSERERDEARQRQQQAASAPASR"
     gene            2036..2695
                     /gene="plsY"
                     /locus_tag="VF453_10750"
     CDS             2036..2695
                     /gene="plsY"
                     /locus_tag="VF453_10750"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX7688179.1"
                     /translation="MALFWSVCVVVLAYLLGSFSFAVIVSRAMGLDDPRSYGSGNPGA
                     TNVLRSGNKLAAILTLLLDALKGVAAVVIARLLAERLGYGEAIVAAAGLASFLGHLYP
                     VFFRFQGGKGVATAAGVLLALDWGLGLASLGTWLAIALLTRYSSLAALVAAVLAPVYV
                     QLMHGSTQRTVAVAVMSVLLVWRHRANISKLLNGTESKLGSKAKAPPPAGDDDAGDSP
                     S"
     gene            2728..3090
                     /locus_tag="VF453_10755"
     CDS             2728..3090
                     /locus_tag="VF453_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7688180.1"
                     /translation="MSNLQRFDVGARLSEMAVYNGTAYLAGQVPADATQDIAGQTRQV
                     LAAIDTLLARAGTDKTRILMAQIFLSELGDFAGMNAAWDDWVPAGHTPPRATVVAKLA
                     KPEWKVEIVITAAVPAAA"
     gene            complement(3127..3597)
                     /locus_tag="VF453_10760"
     CDS             complement(3127..3597)
                     /locus_tag="VF453_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688181.1"
                     /translation="MEDDAVRVHEDRLWIDNGWTARVVKNEDDDGWAVAMTRDGEREP
                     ALVGPWTMGRDKKNPKPLDTNAFNTLVKTAGEVIRRHEQALHAAMHKQFEVWTGEGRV
                     LVTLDIVPDEYDPHALLAAQDEFGDPLARVKVRPDFKLTKASAEAWVESGYAKP"
     gene            3843..>4034
                     /locus_tag="VF453_10765"
     CDS             3843..>4034
                     /locus_tag="VF453_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX7688182.1"
                     /translation="MDWPASTWWWIIAGLLVAGELATGTFYLLMVAFGGAAAAIAAQL
                     GGGEGSQVAAAAIVGGGAVI"
BASE COUNT          493 a         1457 c         1473 g          611 t
ORIGIN      
        1 cgggctcttc gcgcgcaggc gcagcgtcac ctcgacgcgt tcgtccgcgc gcgccgggcc
       61 gatcgcgcgc gcgccgggca gcggctgctt gtcgctgccc gggatgatgt gtcggttctt
      121 gctgctggcc atgttgatcc ccttgcgtga tggtggttcc cgcgtccgcg gcgggctgct
      181 cgcgggaggg gccatcctcg atccgttcgc ggatgcagtc cacccctagt cgaccaaaag
      241 ccccgacgcc gctggggctc gaattggggg gatgccccgc cccgtatcgg cccggcggcg
      301 cggtcggccc gcggcgcaac gctcccgggc agatcccgcg cccgctctcg agcaggttgc
      361 cgtcctgctg gctcaaggcg tgcgcctgcc gccgacgccg cgcggcgcga gccgtcggag
      421 ggaggtcggc ttgcgccggc agaccctggc gcgcgccttg ctgtaaccgg acggcggcgg
      481 atgccgatgt cgggcgctga gcgtcccgtc gcgcgcggac ggcgtttcgt cggccggccc
      541 cttgacgacc ggcggtgcgg gccccacctt cgcgcctgga aacgtgccgc cggcgcctgc
      601 cgcggccctt ccgctgaaag gacatgacga tgaaacgaca cctcctgatc ggcgccgccc
      661 tgctgtggtc ggccgtcgcg ttcgccgtgc catcgcgggc ggacgtcgag tccgccgtca
      721 aggccggcga ctacgcgcgc gccgagacca tgatgcgcga ggtcgtgacc gcggatccgc
      781 agagcgcgcg ggcgcactac tatttcgccg aggtgctcgc gcacgagggc aagttcgacg
      841 acgccgtcgg ccaggcccgc caggcccggc agctcgaccc caggatcggc ttcaccgatc
      901 ccgcgaagtt cacccagttc gagcagctgc tgcgccgcga gcagtcgcac gccggcacga
      961 ccaccccggc gccgggcacg ctgccggccg ccaccgatgc cgtgccggcg cccgcgcccg
     1021 cgccggtcgt gcgcgcgaca tcgggcatgc cgggctgggt ctgggtgctc ggcatcgcgg
     1081 ccgtgctgtt cttcgtctgg cgcatggcga cgcgtcgcct cgcgtcctcg ccttcgatgc
     1141 agcccgcggg ctacggcggt gcgggcttcg ctcagccggg atacggccag cccggctatg
     1201 gacagccagg ctacggacag ccgggctacg gacagccggg ctatgggcag cccggctacg
     1261 gccccggcta tccgcagccg ggtgcgggcg gcggcatgct gcgcacgggc ctggccgcgg
     1321 ctggtggcct cgcggccggc atggcgctgg agcacatgct cgacgagcgc cacgagcgcg
     1381 accgctacgt cgacgacaac ctcgccggcg gcaacgtcgg cggcggcatc ccggccccgt
     1441 tcgatgacga ctccagccgc gccgccaccg agctggagaa ccgcccgttc gacttcggca
     1501 acgatgccgg cggcggctgg gacgacggcg gcggttcggg cggcggctcg tcggacgacg
     1561 gcggctggtg acgccgcgcg ggtgccggct gcggcgcctg gaggaggagg ggacgggcgt
     1621 gcgccgtccc cttttttatt cctggggccg gctcagcggc tcgcgggtgc gctcgcggcc
     1681 tgctgctggc gctgccgcgc ttcgtcgcgc tcgcgctcgg acttcttctt cgcctcgtga
     1741 tgcccgacgg cacagccggc cgccgcgccg atcagcggat gcttgaccac cgcgcccccc
     1801 acgccgccga ccacggcgcc cttcaggcag cccttggcgg aggcgccggg cgcggccagg
     1861 ccggcggcga gcaggacaag gaggggaagg gcggctggtt tcatgacgga cctcgggctc
     1921 ggacgggacg gtgacgccgg ggcggcaaga cgtgggcccg gacgccgggt cgcgccgcgg
     1981 agccggggcc gactacactt gccggtcgtc cgaattcctg cgaaggcgtc tcccggtggc
     2041 tctgttctgg tcggtgtgtg tggtggtgct ggcgtacctg ctggggtcgt tcagcttcgc
     2101 ggtgatcgtc tcgcgggcga tgggcctgga cgatccgcga tcctacggct cgggcaaccc
     2161 gggcgcgacc aacgtgctgc gcagcggcaa caagctcgcc gcgatcctga ccctgctgct
     2221 ggacgcgctc aagggagtgg ccgcggtcgt gatcgcccgc ctgctggccg aacgcctcgg
     2281 ctacggcgag gccatcgtcg ccgcagccgg cctcgcatcc ttcctggggc atctctaccc
     2341 tgtcttcttc cgcttccagg gcggcaaggg cgtcgccacc gccgcgggcg tcctgctggc
     2401 gctcgactgg gggctgggcc tcgcgagcct gggcacctgg ctcgcgatcg cgctgctgac
     2461 gcgctattcg tcgcttgccg cgctggtcgc ggcggtgctg gcgcccgtct acgtccaact
     2521 catgcacggg agcacacaac gcaccgtggc agtggccgtc atgagcgtgc tgctggtctg
     2581 gcgtcatcgc gccaacatct ccaagctgct caacggcacg gagtccaagc tcgggagcaa
     2641 ggccaaggcg ccgcctccgg ccggcgacga cgatgccggc gacagcccct cctgaacccg
     2701 accgtacctg ttccaagagc attccccatg agcaacctcc aacgattcga cgtcggcgcc
     2761 cgcctgtccg agatggccgt ctacaacggc acggcctacc tcgccggcca ggtgccggcc
     2821 gacgcgacgc aggacatcgc cggccagacc cgccaggtgc tggccgcgat cgacacgctg
     2881 ctggcccgtg ccggcaccga caagacgcgc atcctgatgg cgcagatctt cctgtccgag
     2941 ctcggcgact tcgccggcat gaacgcggcg tgggacgact gggtgcctgc cggccacacg
     3001 ccgccgcgcg cgaccgtcgt cgccaagttg gccaagcccg agtggaaggt cgagatcgtc
     3061 atcaccgcgg cggtgcctgc ggcggcctga gcgccagcga ccggctgctc gtctcgccgt
     3121 tcgggatcag ggcttggcgt agccgctctc gacccaggcc tccgcactgg ccttcgtcag
     3181 cttgaaatcg gggcgcacct tgacgcgcgc gagcgggtcg ccgaattcgt cctgcgcagc
     3241 gagcagggcg tgcgggtcgt actcgtcggg cacgatgtcc agcgtgacga gcacgcggcc
     3301 ctcgcccgtc cagacctcga actgcttgtg catggcggcg tgcagggcct gctcgtggcg
     3361 gcggatgacc tcgccggccg tcttcacgag cgtattgaac gcgttcgtgt cgagcggctt
     3421 cgggttcttc ttgtcacggc ccatcgtcca ggggccgacc agcgccggct cgcgttcgcc
     3481 gtcgcgcgtc atcgccacgg cccagccgtc gtcatcctcg ttcttgacga cgcgggccgt
     3541 ccagccgttg tcgatccaga ggcgatcctc gtggacgcgg acggcgtcgt cctccatgtc
     3601 gggcgccgag ggcgcgacgg cctgggcatc gcccgccggc gaaccggcgg ccatggcgcg
     3661 ggcgtgctct gaagcgtatt cgaaagcctg ttgttccata cagtgatgat aagcgcaacg
     3721 ggcgcgcatg cgcaacggcg gaacggcgcg tcaacacccc gtttccgcgc gcggcgccgg
     3781 cccggacctg ccgtagcatg tcgactgcgg tcgcgttggc cgcgccgacg aggaaggcat
     3841 gcatggactg gcccgcatcg acgtggtggt ggatcatcgc cggcctgctg gtggcgggcg
     3901 agctggccac gggcaccttc tacctgctga tggtggcctt cggcggcgcg gcggcggcga
     3961 tcgccgcgca gctgggcggc ggcgagggga gccaggtggc ggcggcggcg atcgtcggcg
     4021 gcggcgcggt gatc
//
LOCUS       DASVLN010000440         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_507369, whole genome shotgun sequence.
ACCESSION   DASVLN010000440 DASVLN010000000
VERSION     DASVLN010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507369"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..887
                     /locus_tag="VF453_10770"
     CDS             195..887
                     /locus_tag="VF453_10770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7688183.1"
                     /translation="MGSTTTFKRPDGKEVQGYLAEPRNPQGAPAVVVIQEWWGLNDQI
                     RGVADRLAHAGFVALVPDLYRGKITVEEEEAHHLMTGLDFGDAASQDVRGAVQHLKAG
                     GARVGVLGFCMGGALALLAAQTSPELDAAVVWYGCPPLEYIDAARIRVPLQGHWATQD
                     EFFKHEQVKQLEAKLQEAGVEFEFHHYLARHAFGNEQAVGEGRIAATQYDPVWAQRAW
                     DRTFAFLARKLA"
     gene            complement(960..>2247)
                     /locus_tag="VF453_10775"
     CDS             complement(960..>2247)
                     /locus_tag="VF453_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688184.1"
                     /translation="GPASTIPAATILSFMTDGPQRNGPWPPGGSVPCDVAIRRLVYWT
                     VAPDGSMLQASAGLLVPAGTADACTHGAFPLVSFQHGTSDTAAFDASDPTATLPLTTA
                     AYFASHGYVTVIPDYLGYGAAASLGYHPYVVAESEASTVIDAVRAARAFFATAGAGAE
                     TLGGQLFLAGTSEGGYVTMATQRAMERDFGNEFRLTAVVPISGPYDLPAETLDDVRSA
                     DAAGVSTTGHATFLFTAYNRVYRNIYGATSDIFLAPWAGDVEGLFPGAYGSDDAAIAA
                     CMIPRWLGMPTPPDKVPAACAARTGDATQPLFDAAFLQDYLDDRPGTRGSQARADVAL
                     NTLLPARRWHNVAPMTPCYGAPDPTAARNAQAAGQYFAIPVEDVQDDPQAPAFIESWA
                     AAAAARLPDYHGQVEAPACTAWARYAVFDRYWPGHL"
BASE COUNT          313 a          794 c          864 g          276 t
ORIGIN      
        1 gcagcgcctc gacaccggct gcgaacgccg gccacccgac gtgcgcctgc ggccgacgcg
       61 ccaatcccgc cttcgcacgc gcgacacgcg aagcgcctat gcttgtcccc ctccgcggcc
      121 ggccccgggg ccggcgcccc acccttccac cccgcgcggc cgagcggcgc gcgcacatga
      181 acgaggagat ccccatgggc agcaccacga cgttcaagcg tcccgacggc aaggaggtgc
      241 agggctacct ggccgagccg cgcaacccgc agggcgcgcc ggcggtcgtc gtgatccagg
      301 aatggtgggg cctgaacgac cagatccgcg gcgtggccga ccgcctcgcg catgccggct
      361 tcgtcgccct ggtgcccgac ctctaccgcg gcaagatcac cgtcgaggag gaagaagcgc
      421 accacctgat gaccgggctg gacttcggcg acgccgcgag ccaggacgtg cgcggcgcgg
      481 tccagcacct gaaggcgggc ggcgcccgcg tcggcgtgct gggcttctgc atgggcggcg
      541 cgctcgcgct gctggccgcg cagacgtcgc ccgagctcga cgccgcggtc gtctggtacg
      601 gctgcccgcc gctggaatac atcgacgcgg ccaggatccg cgtgccgctg cagggccact
      661 gggcgacgca ggacgagttc ttcaagcacg agcaggtgaa gcagctcgag gcgaagctcc
      721 aggaggccgg ggtcgagttc gagttccacc actacctcgc ccgccacgcg ttcggcaacg
      781 agcaggccgt cggcgaaggc cgcatcgcgg cgacgcagta cgacccggtg tgggcgcagc
      841 gggcctggga tcgcacgttc gcgttccttg cccgcaagct cgcctgaagc ccgggacgcc
      901 gggagcgcac gcgaaaaatc ccgggccggc cgacgctccc acgccgtcgg cccgggcggt
      961 caaaggtggc cgggccagta gcggtcgaag acggcgtagc gcgcccaggc cgtgcaggcc
     1021 ggggcctcga cctggccgtg gtagtcgggc aggcgggccg cggccgcggc ggcccagctc
     1081 tcgatgaagg ccggtgcctg cgggtcgtcc tgcacgtcct cgaccgggat ggcgaagtac
     1141 tggcccgcgg cctgcgcgtt gcgggcggcg gtcgggtcgg gcgcgccgta gcagggcgtc
     1201 atcggcgcga cgttgtgcca gcgccgggcg ggcagcagcg tgttgagcgc gacgtcggcg
     1261 cgcgcctggc tgccgcgcgt tcccggccgg tcgtccaggt agtcctgcag gaacgccgcg
     1321 tcgaacagcg gctgcgtggc gtcgcccgtc ctcgcggcgc aggcggccgg caccttgtcg
     1381 ggcggcgtcg gcatgccgag ccagcgcggg atcatgcagg ccgcgatcgc ggcgtcgtcg
     1441 ctgccgtagg caccgggaaa cagtccctcg acatccccgg cccacggggc gaggaagatg
     1501 tcggacgtcg cgccgtagat gttgcgatag acgcggttgt aggccgtgaa caggaaggtc
     1561 gcgtggcccg tggtggacac gcccgccgcg tcggcggagc gcacgtcgtc cagcgtctcg
     1621 gcgggcaggt cgtaaggccc ggagatcggg acgaccgcgg tcaggcggaa ctcgttgccg
     1681 aagtcgcgct ccatcgcgcg ctgcgtggcc atggtgacgt agccgccctc ggaggtgccg
     1741 gcgaggaaca gctggccgcc cagggtctcg gcgccggcac cggccgtcgc gaagaacgcc
     1801 cgcgcggcgc gcaccgcgtc gatcaccgtg gacgcttccg actcggcgac cacgtacggg
     1861 tggtagccga ggctcgcggc cgcgccgtag ccgaggtagt cgggaatgac cgtcacgtag
     1921 ccgtgcgagg cgaagtaggc ggccgtggtc agcggcagcg tggccgtcgg atcggaggcg
     1981 tcgaaggccg ccgtgtcgga cgtgccgtgc tggaagctca cgagcgggaa tgcgccgtgc
     2041 gtgcaggcgt ccgccgtgcc ggccggcacg agcagtcccg cgctcgcctg cagcatcgag
     2101 ccgtcgggcg cgaccgtcca gtagacgagg cggcggatcg cgacgtcgca gggcacgctg
     2161 ccgccgggag gccacgggcc gttgcgctgc gggccgtccg tcatgaacga caggatcgtc
     2221 gccgccggga tcgtcgaggc cgggccg
//
LOCUS       DASVLN010000441         6713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_130925, whole genome shotgun sequence.
ACCESSION   DASVLN010000441 DASVLN010000000
VERSION     DASVLN010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130925"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2812
                     /gene="gcvP"
                     /locus_tag="VF453_10780"
     CDS             <1..2812
                     /gene="gcvP"
                     /locus_tag="VF453_10780"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX7688185.1"
                     /translation="LSAIGAASRAALVDALVPRNIVRGAPMAIPAPVTEAGALAELRG
                     LAAKNKVLKSFIGQGYFGTLTPGVILRNILENPAWYTAYTPYQAEISQGRMEALLNFQ
                     TMVTDLTGMAIANASMLDEATAAAEAMTLAQRMGKSKSKRFFVADDVLPQTLEVVRTR
                     ARPLDIEVAVGPAETAGEHDCFAALFQYPGVNGAVRDLGPLVEAVHARGGLAVVAADL
                     LALTLLKSPGEFGADIACGNTQRFGMPMGCGGPHAAYLATQDALKRSLPGRLVGVSVD
                     THGAPAYRLALQTREQHIRREKATSNICTAQVLPAVVASMYAVYHGPEGLRRIARRVA
                     TYAAILAAGLRQRGVAVANAHAFDTLEVKTGPSTDAALARAVVANMNLRRASADSVSV
                     SLDETTTRADLAALWEVFAPGQAAPSFEGFEAGVEPLLPAALLRTSGFLSHPVFNTHH
                     SETEMLRYIRRLSDKDLALDRSMIPLGSCTMKLNATSEMIPITWPEFAQVHPFAPADQ
                     LEGYRLLNEQLCGWLEQATGYAGVSLQPNAGSQGEYAGLLVIKAWHASRGEGARDVCL
                     IPESAHGTNPASAQMVGMKVVVVKCTPEGSVDMADLEAKCAAHKDDLAAMMITYPSTY
                     GVFEPGVKDLCALVHKYGGRVYVDGANMNAMVGIAAPGQFGGDVSHLNLHKTFCIPHG
                     GGGPGVGPVCVVEDLVPFLPAHRSAGLGADDDRRVGAVSAAPLGNAAVLPISWMYVRM
                     MGASGLKAATETAILNANYVAARLSAHYDIHFSGGVAGVKGGGVAHECILDLRPLKDS
                     SGVTAEDVAKRLIDYGFHAPTLSFPVAGTLMVEPTESEPLVELDRFCDAMIAIRAEIA
                     KVEAGSWPREDNPLKNAPHTAQSLLKADWAHAYSREDAAYPLVALRAGKYWSPVGRVD
                     NVYGDRNLFCSCVPMSEFRNDE"
     gene            2882..4273
                     /locus_tag="VF453_10785"
     CDS             2882..4273
                     /locus_tag="VF453_10785"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEX7688186.1"
                     /translation="MAVSVFDLFKIGIGPSSSHTVGPMRAARLFVARLAHEGALERTA
                     RVKADLYGSLGATGKGHGSDKAVLLGLAGHEPDTVDIEQVPALLDAIRERHRVALNGS
                     REIAFDESADLVMHRRETLPFHANGMRFTAYDDTGAELENRVYYSVGGGFVVSDEVAA
                     DGTRQKTIAPDTTVLPYPFHSGDDLLRLAQEHDLSIAELMRRNERHWRSDAELHDGLM
                     KIWHVMQECVARGLRTEGVLPGGFKVKRRAAGLYRALTANPEAALRDPLQVMDWVNLY
                     ALAVNEENAAGGRVVTAPTNGAAGIVPAVLHYYTRFVPGATDAGVVDFMLTAAAIGIL
                     YKENASISGAEVGCQGEVGVACSMAAAGLAAVMGGTPRQVENAAEIGMEHHLGLTCDP
                     VGGLVQIPCIERNAIASVKAINAARMALRGDGTHHVSLDKVIKTMRETGADMQTKYKE
                     TARGGLAVNIVEC"
     gene            4403..6103
                     /locus_tag="VF453_10790"
     CDS             4403..6103
                     /locus_tag="VF453_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7688187.1"
                     /translation="MTSTSNGDPVTDSEVRQALEEVVDTAMAITGAGFGNVQLLDPAT
                     GDLRIVAQRGFPAWWLEYWNAVAKGHGACGTALARRERVVVEDVERSPIFVGTPGLDI
                     QRRAGVRAVQSTPVFSRAGAPLGMFSTHFRAVHQPDGQAQKLLDLLARHVGELVERFR
                     AEDEIRKLNAELRLRVTELEEARSRAEQLAHSKSAFLANMSHEIRTPMNAILGMAHLM
                     RKDGLTPMQSGRVDHIATAAEHLVHLVGDILDMSKIEAGKLRLEQSPFMLDEVLARVV
                     AMVAPRAAEKGLELRSDAPPLPHALVGDAMRLTQALLNYVNNAVKFTDQGSVTLRSRP
                     VQKTPERTLIRFEVEDSGIGIAPDQLRRLFNAFEQADDSTTRKYGGTGLGLAITRRLA
                     RLMEGDAGVSSVPDVGSVFWFTAWFANGDALPAAGAGAADGEPPDVVLAREHAGRRVL
                     LVEDDPVNQLITSEMVSGMGLVVDKAGNGLEAVSQAQARPYDLILMDVQMPHLDGMEA
                     TRSIRALPGFASVPIIALTAGGIAEVRDKCRAAGLDDLLSKPTPPRTLAAVLLKWLSR
                     "
     gene            complement(6340..>6713)
                     /locus_tag="VF453_10795"
     CDS             complement(6340..>6713)
                     /locus_tag="VF453_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688188.1"
                     /translation="AVHAAAPASAVAAQAAPPRSEGSADQRILARYAQQDRVAALERD
                     LKSVRDEIAQRPSQKAADVAAVRTAYQRSLDAGEPGAPAVLNAALRSIEDKYRAMAEL
                     DRQRLEAAQAAVDKARAQPTP"
BASE COUNT         1001 a         2501 c         2310 g          901 t
ORIGIN      
        1 gctgtccgcc atcggcgccg cctcgcgcgc cgcgctggtg gacgcgctgg tgccgcgcaa
       61 catcgtgcgc ggcgcgccga tggcgatccc cgcgccggtc accgaggccg gcgcgctcgc
      121 cgagctccgc ggcctcgcgg cgaagaacaa ggtgctcaag agcttcatcg gccagggcta
      181 cttcggcacg ctcacgccgg gcgtcatcct gcgcaacatc ctcgagaacc cggcctggta
      241 caccgcctac acgccctacc aggccgagat ctcgcagggc cgcatggagg cgctgctgaa
      301 cttccagacc atggtcaccg acctcacggg catggccatc gccaacgcgt ccatgctcga
      361 cgaggcgacc gccgccgccg aggcgatgac cctggcgcag cgcatgggca agtcgaagtc
      421 gaagcgcttc ttcgtcgccg acgacgtcct gccgcagacg ctggaagtcg tgcgcacgcg
      481 cgcgcggccg ctggacatcg aggtcgcggt cggcccggcc gagaccgcgg gcgagcacga
      541 ctgcttcgcc gccctcttcc agtacccggg cgtcaacggc gccgtgcgcg acctcgggcc
      601 actggtcgag gcggtgcatg cgcgcggcgg cctcgcggtc gtcgcggccg acctgctcgc
      661 gctgacgctg ctgaagtcgc ccggcgaatt cggcgccgac atcgcctgcg gcaacacgca
      721 gcgattcggc atgccgatgg gctgcggcgg cccgcacgcc gcctacctcg cgacgcagga
      781 cgcgctcaag cgctcgctgc cgggccgcct ggtcggcgtc agcgtggaca cgcacggcgc
      841 gcccgcctac cgcctcgcgc tgcagacgcg cgagcagcac atccgccgcg agaaggccac
      901 ctccaacatc tgcacggcgc aggtgctgcc ggcggtcgtg gccagcatgt acgccgtcta
      961 ccacggcccc gagggcctgc gccgcatcgc gcgccgcgtg gcgacgtatg ccgcgatcct
     1021 cgccgccggc ctgcgccagc gcggcgtcgc cgtcgccaac gcgcacgcgt tcgacacgct
     1081 cgaggtgaag accggcccgt cgaccgacgc ggccctcgcg cgcgccgtcg tggcgaacat
     1141 gaacctgcgc cgcgcgagcg ccgactcggt gtccgtgtcg ctggacgaga cgaccacgcg
     1201 cgccgacctc gccgcgctgt gggaggtctt cgcgccgggc caggccgcgc cgtcgttcga
     1261 gggcttcgag gccggcgtcg aacccctgct gcccgccgcg ctgctgcgca cgagcggctt
     1321 cctctcgcac ccggtcttca acacccacca cagcgagacg gagatgctgc gctacatccg
     1381 ccgcctctcc gacaaggacc tcgcgctcga ccgcagcatg atcccgctcg gctcgtgcac
     1441 gatgaagctc aacgcgacga gcgagatgat tcccatcacc tggcccgagt tcgcgcaggt
     1501 gcatccgttc gcgccggccg accagctcga aggctaccgc ctgctcaacg agcagctgtg
     1561 cggctggctc gagcaggcca ccggctacgc gggcgtgagc ctgcagccca acgcgggctc
     1621 gcagggcgag tacgcggggc tgctggtcat caaggcctgg catgcctcgc gcggcgaggg
     1681 cgcgcgcgac gtctgcctga tccccgagtc cgcgcacggc accaacccgg cctcggcgca
     1741 gatggtgggc atgaaggtcg tcgtcgtgaa gtgcacgccc gagggcagcg tcgacatggc
     1801 cgacctcgag gccaagtgcg ccgcgcacaa ggacgacctc gccgcgatga tgatcaccta
     1861 cccctccacg tatggcgtgt tcgagcccgg cgtgaaggac ctgtgcgccc tcgtgcacaa
     1921 gtacggcggt cgcgtctacg tcgacggcgc caacatgaac gcgatggtgg gcatcgcggc
     1981 gccgggccag ttcggcggcg acgtcagcca cctgaacctg cacaagacgt tctgcatccc
     2041 gcacggcggc ggcggcccgg gtgtcggccc ggtctgcgtg gtcgaggatc tcgtgccctt
     2101 cctgccggcc caccgctcgg ccgggctggg cgcggacgac gaccggcgcg tcggcgccgt
     2161 ctcggccgcg ccgctgggca acgccgcggt gctgccgatc agctggatgt acgtgcgcat
     2221 gatgggcgcg tccggcctca aggcggccac cgagacggcg atcctcaacg ccaactacgt
     2281 cgccgcgcgc ctgtccgcgc actacgacat ccacttcagc ggcggcgtcg ccggcgtgaa
     2341 gggcggcggc gtcgcgcacg aatgcatcct cgacctgcgc ccgctcaagg acagctccgg
     2401 cgtcaccgcc gaggacgtgg ccaagcgcct gatcgactac ggcttccacg cgccgacgct
     2461 gtcgttcccg gtcgcgggca cgctgatggt cgagcccacc gagagcgagc ccctcgtcga
     2521 gctcgatcgc ttctgcgacg cgatgatcgc catccgcgcc gagatcgcca aggtcgaggc
     2581 cggcagctgg ccgcgcgagg acaacccgct gaagaacgcg ccgcacaccg cgcagtcgct
     2641 gctgaaggcc gactgggcgc acgcgtactc gcgcgaggac gccgcctacc cgctggtggc
     2701 gctgcgcgcg ggcaagtact ggtcgccggt gggtcgcgtc gacaacgtct acggcgaccg
     2761 caacctgttc tgcagctgcg tccccatgtc cgaattccgg aacgacgagt aagcccctcg
     2821 ccggacgcgc cgtcaagagc gtcgcgtccg gcctccctcc gatcgcccac gaggtcctgc
     2881 catggccgtc tccgtcttcg acctcttcaa gatcggcatc gggccgtcga gctcgcacac
     2941 cgtcgggccg atgcgcgcgg cgcggctgtt cgtcgcccgc ctcgcgcacg agggcgcgct
     3001 cgagcgcacg gcgcgcgtga aggccgacct ctacggctcg ctgggcgcca ccggcaaggg
     3061 ccacggcagc gacaaggcgg tgctgctggg cctcgcgggg cacgagcccg acacggtcga
     3121 catcgagcag gtgccggccc tcctcgacgc catccgcgaa cgccatcgcg tggcgctgaa
     3181 cggcagccgc gagatcgcgt tcgacgagtc cgccgacctc gtgatgcacc ggcgcgagac
     3241 cctgcccttc cacgccaacg gcatgcgttt caccgcgtac gacgacacgg gcgccgagct
     3301 cgagaaccgc gtgtactact cggtgggcgg cggcttcgtc gtcagcgacg aggtcgcggc
     3361 cgacggcacg cggcagaaga cgatcgcgcc cgacaccacc gtcctgccct accccttcca
     3421 cagcggcgac gacctgctgc gcctggcgca ggagcatgac ctgtcgatcg ccgagctgat
     3481 gcgccgcaac gagcgccact ggcgcagcga cgccgagctc cacgacgggc tcatgaagat
     3541 ctggcacgtg atgcaggagt gcgtcgcgcg cggcctgcgc accgagggcg tgctgcccgg
     3601 cggcttcaag gtcaagcgcc gcgccgccgg cctgtaccgc gcgctgacgg ccaatccgga
     3661 agccgcgctg cgcgacccgc tgcaggtgat ggactgggtc aacctctatg cgctggccgt
     3721 caacgaggag aacgccgccg gcggccgcgt cgtcaccgcg cccaccaacg gcgccgccgg
     3781 catcgtgccg gccgtcctgc actactacac gcgcttcgtg cccggcgcga ccgacgccgg
     3841 cgtcgtcgac ttcatgctga cggccgccgc gatcggcatc ctctacaagg agaacgcctc
     3901 gatctcgggc gccgaggtcg gctgccaggg cgaggtcggc gtcgcctgct cgatggccgc
     3961 ggcgggcctg gccgccgtga tgggcggcac gccgcgccag gtcgagaacg ccgccgagat
     4021 cggcatggag caccatctcg gcctcacgtg cgacccggtc ggcggactgg tgcagatccc
     4081 ctgcatcgag cgcaacgcca tcgcgtcggt caaggcgatc aacgccgcgc gcatggccct
     4141 gcgcggcgac ggcacccacc acgtgagcct cgacaaggtc atcaagacga tgcgcgagac
     4201 cggggcggac atgcagacca agtacaagga gacggcgcgg ggcgggctgg cggtgaacat
     4261 cgtcgagtgc tgaacccggc gcgcccgtgg cggtcggcca cccggcgcgg cttgagtgcc
     4321 ctcaaccgcg ccatgcccgc ccgcggtatg ctcagcggta cctgctgcgg gcgcggtgct
     4381 cggtaaggta gccggcaaga cgatgacctc cacgagcaac ggcgatccgg tgaccgacag
     4441 cgaggtgcgg caggccctcg aggaggtcgt cgacacggcc atggccatca ccggcgccgg
     4501 cttcggcaac gtccagctgc tcgatccggc caccggcgac ctgcgcatcg tggcgcagcg
     4561 cggctttccc gcgtggtggc tcgagtactg gaacgccgtc gccaagggcc acggcgcctg
     4621 cggcacggcg ctggctcgtc gcgagcgcgt ggtcgtcgag gacgtcgagc gcagccccat
     4681 cttcgtgggc acgccgggac tggacatcca gcgccgcgcc ggcgtgcgcg ccgtccagtc
     4741 gacgccggtc ttcagccggg ccggggcgcc gctgggcatg ttctccacgc acttccgggc
     4801 cgtgcaccag cccgacgggc aagcccagaa gctgctcgac ctgctcgcac ggcacgtcgg
     4861 cgagctggtc gagcgtttcc gcgcggaaga cgagatccgc aagctcaacg cggaactccg
     4921 acttcgtgtg acggagctgg aggaagcccg ctcccgcgcg gagcagctgg cgcacagcaa
     4981 gagcgccttc ctcgccaaca tgagccacga gatccgcacg ccgatgaacg ccatcctcgg
     5041 catggcgcac ctcatgcgca aggacgggct cacgccgatg cagtccgggc gggtcgacca
     5101 catcgcgacg gccgcagagc acctcgtgca cctcgtcggc gacatcctcg acatgtcgaa
     5161 gatcgaagcg ggcaagctca ggctcgagca aagccccttc atgctcgacg aggtcctcgc
     5221 ccgggtcgtc gccatggtcg cgccgcgcgc cgccgagaag ggactcgaac tgcgcagcga
     5281 cgcgccgccg ctgccccatg cgctggtcgg cgacgcgatg cgcctgaccc aagccctgct
     5341 caactatgtg aacaacgccg tgaagttcac cgaccaggga agcgtcacgc tgcgcagccg
     5401 cccggtgcag aaaacgccgg agcggacgct gatccgcttc gaggtggagg acagcgggat
     5461 cggcatcgcg cccgatcagc tcaggcgcct gttcaacgcc ttcgagcagg cggacgactc
     5521 gaccacgcgc aagtacggcg gcaccggcct gggcctggcg atcacccggc ggctcgcgcg
     5581 gctgatggaa ggcgacgccg gcgtcagcag cgtgccagat gtcggcagcg tcttctggtt
     5641 caccgcctgg ttcgcgaacg gcgacgccct gcccgcggcg ggcgccggcg cggcggacgg
     5701 cgaaccgccg gacgtcgtgc tggcgcgcga gcatgccggc cgccgcgtgc tgcttgtcga
     5761 ggacgatccg gtcaaccagc tgatcacctc cgagatggtg agcggcatgg gcctggtcgt
     5821 ggacaaggcc ggcaacggcc tcgaggccgt gagccaggcg caagcgcgtc cctacgacct
     5881 gatcctcatg gacgtgcaga tgccgcactt ggacggcatg gaggcgacgc gctccatacg
     5941 cgcgcttccc ggcttcgcgt ccgtgccgat catcgcgctc acggccggcg gcatcgcgga
     6001 ggtccgcgac aagtgccgcg cggccggcct ggacgacctg ttgtccaagc cgactccgcc
     6061 gcgcacgctc gccgccgtcc ttctgaagtg gctgtcccgc taggccgcgc ctcaagttcg
     6121 cggccacgaa agaccgcgac ccatcagatc ggccacatcc tctcgatcgc gttcagcgac
     6181 ggccacgtcg agcacgtcga ctttcgcgcc gttcatcttc gcgtcgcgac atccaggcta
     6241 cgaaccgtac gagtcggtcg agcgctgcaa gcgcttcgac ctcgtcgacg gcgacctcaa
     6301 ctggctcaca acaggatctc cggacggcgc tgacggccgt cagggcgtcg gctgcgccct
     6361 cgccttgtcc accgccgcct gcgccgcctc gagccgctgg cggtcgagtt ccgccatcgc
     6421 gcggtacttg tcctcgatcg agcgcagcgc ggcgttcagg acggccggcg cgccgggttc
     6481 gccggcgtcc agggagcgct ggtaggcggt gcgcacggcg gcgacgtcgg cggccttctg
     6541 gctcggacgc tgggcgatct cgtcgcgcac ggacttcagg tcgcgctcca gcgccgccac
     6601 gcgatcctgc tgcgcgtagc gcgcgaggat gcgctggtcg gcgctgccct ccgatcgagg
     6661 cggggccgcc tgggcggcca ccgcggaggc gggcgccgcc gcgtgcaccg ccg
//
LOCUS       DASVLN010000442         4476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_236823, whole genome shotgun sequence.
ACCESSION   DASVLN010000442 DASVLN010000000
VERSION     DASVLN010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236823"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1666)
                     /locus_tag="VF453_10800"
     CDS             complement(8..1666)
                     /locus_tag="VF453_10800"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX7688189.1"
                     /translation="MNFDYIVVGGGSGGCVLAARLSEDPDVSVALIEAGPADKSVLIH
                     CPAGLALLARTGSANWNFETVPQKGLGGRVGWSPRGKVLGGSSSINAMVYIRGHRADY
                     DHWAAEGNPGWSYDEVLPYFRRAEHNERIADEYHGTGGPLNVMDLRSPNRYLQAFVDA
                     GAQAGYPVNHDFNGAEQEGIGPYQVTHRNGERFSAAKAYLTPSLGRPNLHVITGAQVT
                     RVLFEGRRAAGVEFRRDGRLEQLGARHEVLMAAGALQTPQLLMLSGVGPAADLSKFGI
                     GVVHDLPGVGRNFHDHVDVVQVVDAPRMKETFGVSASAIARITRGIFDWRNRRTGMLT
                     TNFAEAGGFIKSQPSEAIPDLQLHFVIGKLIDHGRKTVLGHGISSHVCLLRPLSRGTV
                     TLASRDPMAAPRIDPNFFDHPDDLARMVRGFRLMRAIMSQPALSRFGGRELPRSAGAV
                     SDAQIEQFIRDYADTIYHPVGTCRMGPGAMDVVGADLRVHGIEGLRVVDASIMPRVVG
                     GNTNAPVIMVAEKASDMIKAARAAGGVASVVQAVGSPAAERIAA"
     gene            complement(1696..2895)
                     /locus_tag="VF453_10805"
     CDS             complement(1696..2895)
                     /locus_tag="VF453_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HEX7688190.1"
                     /translation="MHPSAASAFAYRDRKRFAWILSLLVPMGVAVGPLLYRAWPQVAM
                     LWLPVIFVYCVAPLIDLALGEDRSNPPEEAVPALEADRYYRVVTWVLVPLLWGAFVYG
                     AWFCIRTPLGWGGRLAVTMTTGMVGGFCINLGHELGHKKPAFERWLAKIVLAPTFYGH
                     FTIEHNRGHHRDVATFADPASARMGESIWRFVLREMPGAFVRAWRLEHARMRVDGQPF
                     WSLRNELLQPALITAALWTALVLWLGPAVLAFLVAASLWANFQLTTANYIEHYGLLRV
                     AGPDGRPERCTPRHSWNSNHAFSNWAVFHLQRHADHHAHPARRYQSLRHFEDAPRLPN
                     GYFGMFPVAYLPPLWFALMDARLVAAVHCDAARINFHPPRRAALIRRHGLRELTPPAG
                     SPAQTSP"
     gene            3052..4098
                     /locus_tag="VF453_10810"
     CDS             3052..4098
                     /locus_tag="VF453_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7688191.1"
                     /translation="MPRAAAAHPAHAPLPQPASLVPPIYARLLRMLLQHADVDGDRVL
                     AAAGLDWPTLVTDDRRLSLGTVVRLAEGAMAATRRPWLGLDLGGNAPVSAHGALGYAA
                     VTSRDLREALRVIARYGTTRNDAMAWTCVETASGMTMRAAERAELGVVRGFVIDTTLG
                     AVLRMIETAVGTVPPGLRVDLPVPTPPWREQYQRFGLAQIRFEQPAFAFHVDARDLAL
                     PCIGADAKAHANACRECEEALAEVRGASMAQRVAGLLASVDAGAYPRMAELAARCGIS
                     PRTLIRRLHDDGTTFQALLDAARQQRALWMLQHTDAPVEEIAARLGYQDTSNFSRTVR
                     RWFGATPRELRDRP"
     gene            4095..>4476
                     /locus_tag="VF453_10815"
     CDS             4095..>4476
                     /locus_tag="VF453_10815"
                     /inference="COORDINATES: protein motif:HMM:NF028481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7688192.1"
                     /translation="MSASGIIAGVIELLQFPPAFGLPNGSPFCMKVEVFLRLAGLEYR
                     TVTASPLPAPKGKLPVLRDGGRTIADSEAIVRHLQATYADRIPAALAAPETARELLVR
                     RTIEEHLYFAALRFRWVDDAGARHS"
BASE COUNT          625 a         1625 c         1614 g          612 t
ORIGIN      
        1 gcccgtttca ggccgcgatg cgctcggccg ccggcgaccc gaccgcctgc acgacgctcg
       61 ccaccccgcc ggccgcgcgc gccgccttga tcatgtccga cgccttctcc gcgaccatga
      121 tcaccggcgc gttcgtgttg ccgccgacca cgcgcggcat gatggacgca tcgaccacgc
      181 gcaggccctc gatcccgtgc acgcgcaggt cggcgcccac gacgtccatc gcccccggcc
      241 ccatgcggca ggtgcccacc gggtggtaga tcgtgtcggc gtagtcgcgg atgaactgct
      301 cgatctgcgc gtcgctcacc gcgcccgcgg agcgcggcag ttcccgcccg ccgaaccggg
      361 agagcgccgg ctgcgacatg atcgcgcgca tcagccggaa tccgcgcacc atgcgcgcca
      421 ggtcgtcggg gtggtcgaag aagttggggt cgatgcgggg tgcggccatc ggatcgcggc
      481 tcgccagcgt gaccgtgccg cgcgacagcg gccgcagcag gcacacgtgc gacgagatgc
      541 cgtgccccag caccgtcttg cgcccgtggt cgatcagctt gccgatcacg aagtgcagct
      601 gcaggtcggg gatcgcctcc gacggctggc tcttgatgaa gccgccggcc tcggcgaagt
      661 tggtcgtgag catgccggtg cggcgattgc gccagtcgaa gatgccgcgc gtgatgcgcg
      721 cgatcgccga ggccgagacg ccgaaggtct ccttcatccg gggcgcgtcg acgacctgca
      781 cgacgtcgac gtggtcgtgg aagttgcgcc ccacgccggg caggtcgtgg acgaccccga
      841 tgccgaactt cgagagatcc gcggccgggc cgacgcccga gagcatcagc agctgcggcg
      901 tctgcagcgc gcccgcggcc atcagcactt catgccgggc tccgagctgc tcgaggcggc
      961 cgtcgcggcg gaattccacg cccgcggcgc gccggccctc gaacagcacg cgcgtgacct
     1021 gcgcgcccgt gatgacgtgc aggttcgggc ggccgaggct gggcgtgagg taggccttgg
     1081 cggcgctgaa gcgttcaccg ttcctgtgcg tgacctggta ggggccgatg ccttcctgct
     1141 cggcgccgtt gaagtcgtgg ttgaccggat agcccgcctg cgcgcccgcg tcgacgaagg
     1201 cctgcaggta gcggttgggg ctgcgcaggt ccatcacgtt gagcgggccg cccgtgccgt
     1261 ggtactcgtc ggcgatgcgc tcgttgtgct cggcgcggcg gaagtagggc agcacctcgt
     1321 cgtaggacca gcccgggttg ccctcggcgg cccagtggtc gtagtcggcg cggtggccgc
     1381 ggatgtagac catggcgttg atcgagctcg atccgccgag caccttgccg cgcggcgacc
     1441 agccgacgcg cccgcccagg cccttttgcg ggacggtctc gaagttccag ttcgccgatc
     1501 ccgttcgcgc gagcagggcg agccccgccg gacagtggat cagcacgctc ttgtcggccg
     1561 gcccggcctc gatcagcgcg acgctgacgt ccggatcctc gctcagccgc gccgcgagca
     1621 cgcagccgcc ggatccgcca cccaccacga tgtagtcgaa gttcatgctt gtctctttgc
     1681 tctgcttgtc gtcggtcaag gggaagtctg tgcgggcgat ccggcgggcg gcgtgagctc
     1741 gcgcaacccg tggcggcgta tcagcgcggc ccggcggggc gggtggaaat tgatgcgtgc
     1801 cgcgtcgcag tggaccgcgg cgacgagccg cgcgtccatc agggcgaacc agagcggcgg
     1861 caagtaggcg accgggaaca tgccgaagta gccgttgggc agccgcggcg cgtcctcgaa
     1921 gtggcgcagc gactggtagc ggcgcgccgg gtgggcatgg tggtcggcgt ggcgctgcag
     1981 gtggaacacg gcccagttcg agaaggcgtg gttgctgttc caggaatggc gcggcgtgca
     2041 gcgctcgggt cggccgtcgg gccccgcgac gcgcagcagg ccgtagtgct cgatgtagtt
     2101 ggcggtcgtg agctggaagt tcgcccacag cgaggccgcg accaggaagg cgagcaccgc
     2161 cggtcccagc cacagcacga gcgccgtcca cagcgccgcc gtgatcagcg cgggctgcag
     2221 cagctcgttg cgcagcgacc agaacggctg cccgtcgacg cgcatgcgcg cgtgttccag
     2281 gcgccacgcg cgcacgaagg cgcccggcat ctcgcgcagc acgaagcgcc agatcgactc
     2341 gcccatgcgc gcggacgcgg ggtcggcgaa ggtcgcgacg tcgcggtggt ggccgcggtt
     2401 gtgctcgatc gtgaagtggc cgtagaaggt cggcgcgagc acgatcttcg cgagccagcg
     2461 ctcgaaggcc ggcttcttgt gccccagctc gtggccgagg ttgatgcaga agccgcccac
     2521 catgccggtg gtcatcgtca ccgcgagccg tccgccccat ccgagcggcg tgcgaatgca
     2581 gaaccacgcg ccgtagacga aggcccccca cagcagcggc acgagcaccc aggtgacgac
     2641 gcggtaatag cggtcggcct cgagcgcggg gacggcctcc tcgggcggat tgctgcggtc
     2701 ctcgcccagc gcgaggtcga ttaggggcgc gacgcaatag acgaagatga cgggaagcca
     2761 caacatcgcc acctgcggcc acgcccggta cagcaacggc cccaccgcca cgcccatggg
     2821 caccagcagc gacaggatcc acgcgaagcg cttgcggtcg cggtaggcaa aggcggatgc
     2881 ggcggaggga tgcatggggt gggcgaggcg tcgagcagcg gcggcccgcg gcccagtgtg
     2941 ggctcgcgtc ccggcgcggg gccaccgagc cagctgccac gtgatcgtca taaagtgaca
     3001 aggcctccgc caaccgcctc gtttcaggga agacaccgag aacccgaccc catgccccga
     3061 gccgccgccg cccatcccgc gcacgcgccg ctgccgcagc cagcctcgct cgtgccgccg
     3121 atctacgcgc gcctgctgcg catgctgctg cagcacgcgg atgtcgacgg cgaccgggtg
     3181 ctcgccgccg ccgggctgga ctggccgacc ctcgtcaccg acgaccgtcg cctgagcctg
     3241 ggcaccgtgg tgcgcctggc cgagggcgcg atggccgcga cgcggcggcc ctggctgggg
     3301 ctggacctgg gcggcaacgc gccggtctcg gcgcatggcg cgctcggcta cgccgcggtg
     3361 acgagccgcg acctgcgcga ggcgctgcgg gtcatcgccc gctacggcac gacgcgcaac
     3421 gacgcgatgg cctggacctg cgtcgagacc gcctccggca tgaccatgcg cgccgccgag
     3481 cgcgccgagc tgggcgtcgt gcgcggcttc gtcatcgaca ccacgctggg cgccgtgctg
     3541 cgcatgatcg agacggcggt cggcacggtg ccgccgggcc tgcgcgtcga cctgcccgtg
     3601 ccgaccccgc cctggcgcga gcagtaccag cgcttcggcc tcgcgcagat ccgcttcgag
     3661 cagccggcat tcgcgttcca cgtcgacgcc cgcgacctcg cgctgccctg catcggcgcc
     3721 gacgccaagg cgcacgcgaa cgcctgccgc gagtgcgagg aggcgctcgc cgaggtgcgc
     3781 ggcgcgagca tggcgcagcg cgtcgccggc ctgctcgcga gcgtggacgc cggcgcctac
     3841 ccgcgcatgg ccgagctcgc ggcgcgctgc ggcatctcgc cgcggaccct gattcgccgc
     3901 ctgcacgacg acggcacgac cttccaggcg ctgctcgacg cggcccgcca gcagcgcgcg
     3961 ctgtggatgc tgcagcacac ggatgccccg gtcgaggaga tcgccgctcg cctcggctac
     4021 caggacacct ccaacttcag ccgtaccgtg cgccgctggt tcggcgcgac gccgcgcgag
     4081 ttgcgcgacc ggccttgagc gcctcgggca tcatcgcggg cgtgatcgag ctcctgcaat
     4141 tcccgcccgc gttcggcctg cccaacggca gcccgttctg catgaaggtc gaggttttcc
     4201 tgcgcctggc cggcctcgag taccgaaccg tcaccgcctc gccgctgcct gcgccgaagg
     4261 gcaagctgcc cgtgctgcgc gacggcgggc gcaccatcgc cgattccgag gccatcgtgc
     4321 gccacctgca ggcgacctac gccgaccgca tccccgccgc gctcgccgcg ccggagacgg
     4381 cgcgcgagct gctcgtgcgc cgcacgatcg aggagcacct gtacttcgcc gcgctgcgct
     4441 tccgctgggt cgacgacgcc ggcgcgcggc actcgc
//
LOCUS       DASVLN010000443         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_236859, whole genome shotgun sequence.
ACCESSION   DASVLN010000443 DASVLN010000000
VERSION     DASVLN010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236859"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VF453_10820"
     CDS             <1..349
                     /locus_tag="VF453_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688193.1"
                     /translation="TPAGLFETTPAAEEAVVTPDLRVTLDAAAPGRIAEAVFRGGVPP
                     MSIEGDGPLAADFSWVTQNVRWDPAADAERFFGPTLAEGVARANEQFAKAAEMARTAM
                     GQMLDMLQSRGKR"
     gene            640..2220
                     /gene="ubiB"
                     /locus_tag="VF453_10825"
     CDS             640..2220
                     /gene="ubiB"
                     /locus_tag="VF453_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEX7688194.1"
                     /translation="MRHFTRLAFILWTLLRFGVDEIALGRVRQPWLRAIARIVSIGRS
                     YDEPRGARLRQALERLGPIFIKFGQVLSTRRDLLPADIADELARLQDRVPPFDGAAAR
                     LLVETALLSELGRHFDDVFETFDNTPVASASVAQVHFATVKGGREVAVKVLRPNVLRA
                     IESDLAILHTVARLVERVSVDGKRLRPRDVVAEFDVYLHDELDLIREAANAAQLRRNM
                     DGLDLVLVPEMLWDYCTSRVMVMERMKGVPISQVQRLREAGVDIRKLARDGVTIFFTQ
                     VFRDGFFHADMHPGNIQVSLEPGTFGRYIALDFGIIGTLTESDKDYLAQNFIAFFRRD
                     YKRVAELHIESGWVPPDTRLESLESAVRAVCEPHFDRPLKDISLGQVLLRLFQTSRRF
                     NVEIQPQLVLLQKTLLNVEGLGRQLDPELDLWSTAQPFLERWMHQQVGWRGFLDRLRD
                     EAPRYAHYFPELPRLVHQALGKVVRRVEDELVPAPAALVGELLREQRRTNRLLQALLY
                     MAIGFGAAVVVLRFAGHA"
     gene            2371..>2537
                     /locus_tag="VF453_10830"
     CDS             2371..>2537
                     /locus_tag="VF453_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEX7688195.1"
                     /translation="MPIYAYRCAACGHSRDVLQKMSDAPLTVCPACGAEQFQKQVTAA
                     GFQLKGSGWYVT"
BASE COUNT          344 a          926 c          890 g          377 t
ORIGIN      
        1 cacgccggcc ggcctgttcg agacgacccc ggcggccgag gaggccgtgg tcacgcccga
       61 cctgcgcgtc acgctcgacg ccgcggcccc cggcaggatc gccgaggccg tcttccgcgg
      121 cggcgtgccg ccgatgtcca tcgagggcga cggcccgctc gccgccgact tcagctgggt
      181 cacgcagaac gtgcgctggg atccggccgc cgacgcggag cgcttcttcg gcccgacgct
      241 cgccgagggc gtcgcgcgcg ccaacgagca gttcgccaag gccgccgaga tggcgcgcac
      301 ggcgatgggc cagatgctcg acatgctgca atcgcgcggc aagcgctgag cggagggtga
      361 tgctgccgat cgccgcgcgg gccgagcgcc gggccgcccc aagccggctg tcgccgaggc
      421 gatcgtccgt ccaccggacg gccgttcgtc cacggcgact cccctcgggg gacggagcgc
      481 cgtacgcggc gcgggagggg ttgtcatgat gcgggccgag cgccgggccg ccccaagccg
      541 gctgtcgccg aggcgatcgt ccgtcccccg gacggccgct cgtccacggc gactcccctc
      601 gggggacgga gtgccgtacg cggcacggga ggggtcgtca tgagacattt cacccgcctc
      661 gccttcatcc tgtggacgct gctgcggttc ggcgtcgacg agatcgcgct cggccgcgtg
      721 cgccagccct ggctgcgcgc catcgcgcgc atcgtctcga tcggccgcag ctacgacgag
      781 ccgcgcggcg cgcgcctgcg ccaggcgctc gagcggctgg ggcccatctt catcaagttc
      841 ggccaggtgc tgtcgacgcg gcgcgacctg ctgccggccg acatcgccga cgagctcgcg
      901 cggctgcagg accgcgtccc gcccttcgac ggcgccgcgg cgcggctgct ggtcgagacg
      961 gcgctgctgt ccgagctcgg ccgccacttc gacgacgtct tcgagacctt cgacaacacg
     1021 cccgtggcca gcgcctcggt cgcgcaagtg catttcgcga ccgtcaaggg cgggcgcgag
     1081 gtcgcggtca aggtgctgcg gcccaacgtg ctgcgcgcga tcgagagcga cctcgcgatc
     1141 ctgcacaccg tcgcgcgcct ggtcgagcgc gtgagcgtcg acggcaagcg cctgcgcccg
     1201 cgcgacgtgg tcgcggagtt cgacgtctac ctgcacgacg agctcgacct gatccgcgag
     1261 gccgccaacg ccgcgcagct gcgccgcaac atggacggcc ttgacctcgt gctcgtgccg
     1321 gagatgctgt gggattactg cacctcgcgc gtcatggtga tggagcgcat gaagggcgtg
     1381 cccatcagcc aggtgcagcg gctgcgcgag gccggggtgg acatcaggaa gctcgcgcgc
     1441 gacggcgtca cgatcttctt cacgcaggtc ttccgcgacg gcttcttcca cgccgacatg
     1501 cacccgggca acattcaggt gagcctggag cccggcacct tcgggcgcta catcgcgctc
     1561 gacttcggca tcatcggcac gctcaccgag tccgacaagg actacctggc gcagaacttc
     1621 atcgccttct tccgccgcga ctacaagcgc gtcgccgagc tgcacatcga gagcggctgg
     1681 gtgccgcccg acacccggct ggagtcgctc gagagcgcgg tgcgcgcggt ctgcgagccg
     1741 cacttcgacc ggccgctgaa ggacatctcg ctgggccagg tgctgctgcg gctgtttcag
     1801 acctcgcgcc gcttcaacgt cgagatccag ccgcagctcg tgctgctgca gaagacgctg
     1861 ctcaacgtcg agggcctcgg ccgccagctc gaccccgagc tcgatctctg gagcaccgcc
     1921 cagcccttcc tcgagcgctg gatgcaccag caggtcggat ggcgcggctt cctggaccgc
     1981 ctgcgcgacg aggcgccgcg ctacgcccac tacttccccg agctgccgcg gctcgtgcac
     2041 caggcgctcg gcaaggtcgt gcgccgcgtc gaggacgagc tcgtgccggc gcccgccgcg
     2101 ctggtgggcg agttgctgcg cgagcagcgc cgcaccaacc ggctgctgca ggccctgttg
     2161 tacatggcga tcgggttcgg cgcggcggtg gtcgtcctgc ggttcgccgg ccacgcctga
     2221 ccggcgccgg gggcgcgttt cgggtcgagc cggccttgta gcgtgacgaa aagcccccaa
     2281 tttcgcgatc cgcgcccgcg cgcggccggg cttgcaccgc cgcgacatcc gggcatccta
     2341 taatttccgg tttccaacga gagttcagtc atgccgatct acgcgtatcg ctgcgcagcg
     2401 tgcggccatt cgcgcgatgt gctccagaag atgtcggacg ccccgctcac ggtgtgcccg
     2461 gcgtgcggtg ccgagcagtt ccagaagcag gtgaccgcgg ccggcttcca gctcaagggc
     2521 tcgggctggt acgtgac
//
LOCUS       DASVLN010000444         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_344386, whole genome shotgun sequence.
ACCESSION   DASVLN010000444 DASVLN010000000
VERSION     DASVLN010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344386"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VF453_10835"
     CDS             <1..690
                     /locus_tag="VF453_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7688196.1"
                     /translation="VLARALLAGASGQLRNRATTAGNLLQRTRCPYFYDTDQPCNKRR
                     PGSGCGALDGVSRDHAVIGGSDACIATHPSDMAVAMRVLDATVETVTSDGATRRIPIA
                     QLHRLPGDTPQVEHALAHGELITAVTLPPPAGGVQLYRKVRDRASYAFALVSVAAIVQ
                     PDGTGRVAFGGVAHKPWRVEAAEGALPQGAQAVVQRAFDGARPTRDNAFKLVLAERAL
                     AATLAQARGQS"
     gene            687..2897
                     /gene="paoC"
                     /locus_tag="VF453_10840"
     CDS             687..2897
                     /gene="paoC"
                     /locus_tag="VF453_10840"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HEX7688197.1"
                     /translation="MKFDTPAGTNPIDALRVVGRPTPRVEGPLKTTGTAAYAYEHHDV
                     APDAAYGVVVGAGIAKGRIAAIDTRAARAAPGVLAIVTADNAGKLGQAKRNAAKLLAG
                     PQVDHYHQAVAIVVAETFEQARAAAALLRVDAEPAPGRFDLAALRGDGKPIEETPITG
                     PSDTHVGDFDRAFAEAPVRLDETYTTPDQSHAMMEPHATIAAWHGGKLTLWTSTQMVD
                     WDRTDLARTLGVAKDDVRIVSPYIGGGFGGKLFLRADALLAALAARAAQRPVKVALAR
                     PLMINNTTHRPATIQRIRIGAGRDGRITAIAHESWSGNQPGGSPETAVNQTRLLYAGA
                     NRMSAMRLVELDLPEGNSMRAPGEAPGMMALEIAIDEMAERLGLDPVEFRILNDTQVD
                     PEKPQRRFSQRRLVDCLRLGAERFGWQARQARPAQVRDGPWLVGLGVAAAFRNNLLQK
                     SAARVRLDARGRVTVETDMTDIGTGSYTIIAQTAAEAMGVALEAVDVRLGDSDFPVSC
                     GSGGQFGASNSTSGVYAACMKLRAQVAARAGLDPAQALFEGGHVSAGGRSMALAQAAG
                     AEGLVAEDRIEYGDLDKRYQQSTFGAHFVEVAVDALTGEIRVRRMLAVCAAGRILNPV
                     SARSQVIGAMTMGVGAALSEALHVDKRRGFFVNHDLASYEVPVHADIPHLDAVFLDET
                     DPIASPMKAKGVAELGICGVAAAVANAVHNATGVRVRDYPVTLDKLIAGLPAPA"
     gene            complement(3102..3458)
                     /locus_tag="VF453_10845"
     CDS             complement(3102..3458)
                     /locus_tag="VF453_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7688198.1"
                     /translation="MDGLPPEALEQVAAYFQALSEPTRLQILNLLRGGERNVGEIAHA
                     CDCTAANVSRHLALLTQHGFLEREGRGTAVYYRIADPAVYALCDLVCGNIARHVERSD
                     RERRAFTRRPVVVRRK"
     gene            3604..>3816
                     /locus_tag="VF453_10850"
     CDS             3604..>3816
                     /locus_tag="VF453_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7688199.1"
                     /translation="MTLRFANSAASAVSAPAAAALALALALPALGATALATAVVESDR
                     QPASQAYEGVVQAVRQTTVAAQVPGAV"
BASE COUNT          538 a         1432 c         1365 g          481 t
ORIGIN      
        1 gtgctcgcgc gcgccctgct cgcgggcgcg tcgggccagc tgcgcaatcg cgcgaccacc
       61 gcgggcaacc tgctgcagcg cacgcgctgc ccctacttct acgacaccga ccagccctgc
      121 aacaagcgcc gcccgggcag cggttgcggc gcgctcgacg gcgtgagccg cgaccatgcg
      181 gtgatcggcg gcagcgacgc ctgcatcgcg acgcatccga gcgacatggc cgtggcgatg
      241 cgcgtgctgg acgcgacggt cgagacggtg acgtccgacg gcgcgacgcg gcgcatcccg
      301 atcgcgcagc tgcaccgcct gcccggcgac acgccgcagg tcgagcacgc cttggcgcac
      361 ggcgagctga tcacggcggt gacgctgccg ccgccggccg gtggcgtcca gctgtaccgc
      421 aaggtgcgcg accgcgcgtc gtacgcgttc gcgctggtgt cggtggccgc gatcgtgcag
      481 ccggacggca ccggccgtgt cgcgttcggc ggcgtcgccc acaagccgtg gcgcgtcgag
      541 gccgcggaag gcgcgctgcc gcagggcgcg caggcggtcg tgcaacgggc gttcgacggc
      601 gcccggccga cgcgcgacaa cgcgttcaag ctcgtgctcg ccgagcgtgc gctcgccgcg
      661 acgctcgcgc aggccagggg acagtcatga agttcgatac tcccgcgggt accaacccga
      721 tcgacgcgct gcgcgtcgtc ggccgcccca ccccgcgcgt cgagggtccg ctcaagacca
      781 ccggcaccgc ggcctatgcc tacgagcatc acgacgtcgc gccggacgcg gcctacggcg
      841 tcgtcgtcgg cgcgggcatc gccaagggcc gcatcgccgc gatcgacacc cgcgccgcgc
      901 gcgccgcgcc aggcgtcctg gccatcgtca cggcggacaa cgccggcaag ctcggccagg
      961 ccaagcgcaa cgcggccaag ctgctggcgg ggccgcaggt cgaccactac caccaggcgg
     1021 tcgccatcgt cgtggcggag accttcgagc aggcgcgcgc cgccgccgcg ctgctgcggg
     1081 tggacgccga gcccgcgccg gggcgcttcg accttgccgc cctgcgcggc gacggcaagc
     1141 cgatcgagga gacgccgatc accggcccgt cggacacgca tgtcggcgac ttcgaccgcg
     1201 ccttcgccga ggcccccgtg cggctcgacg agacctacac gacgcccgac cagtcgcacg
     1261 cgatgatgga gccgcatgcg acgatcgccg cgtggcacgg cggcaagctg acgctgtgga
     1321 cgtcgacgca gatggtggac tgggaccgca ccgaccttgc caggacgctg ggcgtggcca
     1381 aggacgacgt gcgcatcgtg tcgccgtaca tcggcggcgg cttcggcggc aagctgttcc
     1441 tgcgcgcgga cgcgctgctc gcggcgctgg ccgcgcgcgc ggcgcagcgg ccggtgaagg
     1501 tcgcgctcgc gcgcccgctg atgatcaaca acacgacgca ccggcccgcg acgatccagc
     1561 gcatccgcat cggggccggc agggacggcc gcatcaccgc gatcgcccac gagagctgga
     1621 gcggcaacca gcccggcgga tcgcccgaga ccgccgtcaa ccagacgcgc ctgctgtacg
     1681 ccggcgccaa ccgcatgagc gcgatgcgcc ttgtcgagct cgacctgccc gagggcaact
     1741 cgatgcgcgc gccgggcgag gcgccaggga tgatggcgct cgagatcgcg atcgacgaga
     1801 tggccgagcg cctggggctc gacccggtcg agttccgcat cctcaacgac acccaggtcg
     1861 atcccgagaa gccccagcgc cgcttctcgc agcgtcgcct ggtcgactgc ctgcgcctcg
     1921 gcgcggaacg cttcggctgg caggcgcgcc aggcccggcc ggcgcaggtg cgcgacggcc
     1981 cctggctcgt cgggctcggc gtggcggccg cgttccgcaa caacctgctg cagaagtccg
     2041 ccgcgcgcgt gcgcctggac gcgcgcgggc gcgtgaccgt cgagaccgac atgacggaca
     2101 tcggcacggg ctcttacacc atcatcgccc agaccgcggc cgaggcgatg ggcgtcgcgc
     2161 tcgaggcggt cgacgtgcgg ctcggcgact cggacttccc cgtctcctgc ggctcgggcg
     2221 gccagttcgg cgcgagcaac tccacctccg gcgtctacgc ggcctgcatg aagctgcgcg
     2281 cgcaggtcgc cgcgcgcgcg ggcctcgacc ccgcgcaggc cctcttcgaa ggcggccacg
     2341 tgagcgcggg cgggcgctcg atggcgctcg cgcaggccgc gggcgcggag gggctggtcg
     2401 ccgaggatcg catcgagtac ggcgacctcg acaagcgcta ccagcagtcg accttcggcg
     2461 cccacttcgt ggaggtggcc gtcgatgcgc tcacgggcga gatccgcgtg cgccgcatgc
     2521 tggccgtgtg cgcggccggc cgcatcctca acccggtctc ggcgcgcagc caggtgatcg
     2581 gcgcgatgac gatgggcgtc ggcgcggcgc tgagcgaggc gctgcacgtc gacaagcggc
     2641 gcggcttctt cgtcaaccac gacctcgcca gctacgaggt gccggtgcac gccgacatcc
     2701 cgcacctcga cgccgtcttc ctcgacgaga ccgacccgat cgcctcgccg atgaaggcca
     2761 agggcgtcgc cgagctgggc atctgcggcg tcgcggccgc cgtcgccaac gcggtccaca
     2821 acgcgaccgg ggtgcgggtg cgggactatc cggtgaccct ggacaagctc atcgccgggc
     2881 tcccggcgcc ggcgtgaccg caggccccgc ccgacgggcc gccggcgccg gtcgcgcccg
     2941 accgcgggtt gcgcattcgc aagcccgccg gatgcccgcg gtgcgaaaag ggcggcatgc
     3001 cgtgcgagcc gctccttccc gtgatcgtcg atgcctcgcc atcgctggtc gcccagcccg
     3061 gcctgccacg ccgcgccggc gcgcgcgcat gcgacgcgac cctacttccg ccgcaccacg
     3121 accggccgcc tggtgaacgc gcggcgctcc cggtcggagc gctccacgtg ccgggcgatg
     3181 ttcccgcaca cgaggtcgca caaggcgtac accgccggat cggcgatgcg gtagtagacc
     3241 gccgtgccgc gcccttcgcg ttccaggaag ccatgctggg tcagcaacgc caggtggcgc
     3301 gacacgttgg cggcggtgca gtcgcaggca tgggcgatct cgcccacgtt gcgctcgccg
     3361 cctcgcagca ggttgaggat ctgcagccgc gtgggctcgg acagcgcctg gaagtacgcg
     3421 gccacttgtt cgagggcctc gggaggcaag ccgtccatcg gatacgtgca ggaagaggtg
     3481 cggggcttca catcatgcct cagcggacat cgccttgccg attatgtgct tgtttgacta
     3541 tttagctgat tgcgcaaata ataaaacata acgacatcga cgaccaccca gggatcgcgg
     3601 accatgacct tgcgcttcgc caactccgcc gcatccgccg tatcggcacc cgccgccgca
     3661 gcgctcgcgc tggccctcgc gctgcccgcg ctcggcgcga cggcgctggc caccgccgtc
     3721 gtcgagagcg accggcagcc tgcgtcccag gcgtacgagg gcgtggtgca ggcggtgcgc
     3781 cagaccaccg tcgccgcgca ggtgcccggc gcggtg
//
LOCUS       DASVLN010000445         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_344449, whole genome shotgun sequence.
ACCESSION   DASVLN010000445 DASVLN010000000
VERSION     DASVLN010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344449"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VF453_10855"
     CDS             <1..802
                     /locus_tag="VF453_10855"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX7688200.1"
                     /translation="TAPAAASAASGTATGVAGAASGVPGPAMQAFEWPPSTRLTYTLT
                     GQYRGGPLYGTAAVEWRRDGAHYQVQFDIHVSPFFDQHMFSDGRIAEDGLRPAHYDEA
                     FKVPVMEPRLRHIEFGDDEVVLNNGNHVARLPGTQDGASQFVQFVWMFATHPQWLRPG
                     NVVDIPLALPHSLRRWRYQVVGTERLALKFGDLDAVHLKPLLDGPRRANEYPFEIWTA
                     PTLQYLPVRIHVQVDEQNYADLSVDQLPLQAAEPPSGTASGAAGNTSP"
     gene            850..1638
                     /locus_tag="VF453_10860"
     CDS             850..1638
                     /locus_tag="VF453_10860"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7688201.1"
                     /translation="MTYECILTETAGAGALKTGVIRLNRPKQMNALSDQLMDELGDAL
                     LAFDRDDGIGAIVVTGNERAFAAGADISTMARYDFMDAYLKGHISRNWETMRQVRKPV
                     IAAVAGFALGGGCEVAMMCDIVIAADTAKFGQPEIKLGIVPGAGGTQRLPRAVGKAKA
                     MDMVLTGRMMDAAEAERSGLVSRVVAADQLMAEALAIGEQLCGLGMPSVMLAKDAVNR
                     SFETGLTEGIAYERRVFHSLFGTADQREGMDAFLAKRKPEFKHR"
BASE COUNT          258 a          632 c          582 g          222 t
ORIGIN      
        1 gaccgcgccg gccgccgcat cggccgcctc cggtacggcg accggcgtgg ccggcgccgc
       61 ctcgggcgtg cccggcccgg cgatgcaggc gttcgagtgg ccgccgtcca cgcggctgac
      121 ctacacgctc accggccagt accgcggcgg cccgctgtac ggcaccgcgg cggtcgaatg
      181 gcgtcgcgac ggcgcgcact accaggtgca gttcgacatc cacgtctcgc cgttcttcga
      241 ccagcacatg ttcagcgacg gccgcatcgc cgaggacggt ctgcgccccg cgcactacga
      301 cgaggccttc aaggtgccgg tcatggagcc gcgcctgcgc cacatcgagt tcggcgacga
      361 cgaggtcgtg ctgaacaacg gcaaccacgt ggcgcggctg ccgggcacgc aggacggcgc
      421 gagccagttc gtgcagttcg tgtggatgtt cgccacgcac ccgcagtggc tgcgccccgg
      481 caacgtcgtg gacatcccgc tcgcgctgcc gcacagcctg cgccgctggc gctaccaggt
      541 ggtcggcacc gagcgcctgg cgctgaagtt cggcgacctc gacgccgtcc acctgaagcc
      601 gctgctggac ggcccccgcc gcgccaacga gtacccgttc gagatctgga ccgcgcccac
      661 gctgcagtac ctgccggtgc gcatccacgt ccaggtcgac gagcagaact acgccgacct
      721 ctcggtcgac cagcttcccc tgcaggccgc cgagccgcca tccggcacgg cgtccggcgc
      781 cgcgggcaac acctcgccct gacgccctcg tacagtccca tcaccccacg accccaccag
      841 gagaccgtca tgacctacga atgcatcctc accgagaccg ccggcgccgg cgcgctcaag
      901 accggcgtca tccggctgaa ccggcccaag cagatgaacg ccctcagcga ccagctgatg
      961 gacgagctcg gcgacgcgct gctcgccttc gatcgcgacg acgggatcgg cgcgatcgtc
     1021 gtgacgggca acgagcgcgc gttcgcggcc ggcgccgaca tctcgaccat ggccaggtac
     1081 gacttcatgg acgcgtacct gaagggccac atctcgcgca actgggagac gatgcgccag
     1141 gtgcgcaagc cggtgatcgc ggccgtcgcg ggcttcgcgc tgggcggcgg ctgcgaggtg
     1201 gcgatgatgt gcgacatcgt gatcgcggcg gacaccgcga agttcggcca gcccgagatc
     1261 aagctgggca tcgtccccgg cgcgggcggc acgcagcgcc tgccgcgcgc cgtcggcaag
     1321 gccaaggcga tggacatggt gctcaccggc cgcatgatgg acgcggcgga agcggagcgc
     1381 tcgggcctgg tctcgcgcgt cgtggcggcc gaccagctga tggccgaggc gctcgcgatc
     1441 ggcgagcagc tgtgcgggct gggcatgccc agcgtgatgc tcgccaagga tgcggtcaac
     1501 cgctcgttcg agaccggcct gaccgagggt atcgcctacg agcgccgcgt cttccattcg
     1561 ctgttcggca ccgccgacca gcgcgagggc atggacgcgt tcctcgccaa gcgcaagccc
     1621 gagttcaagc accgctgaca aggcgcggcg ggccgccgcg cgcgccgcag gccgtggacg
     1681 cctgcctcgg cgcc
//
LOCUS       DASVLN010000446         3207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_237114, whole genome shotgun sequence.
ACCESSION   DASVLN010000446 DASVLN010000000
VERSION     DASVLN010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237114"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VF453_10865"
     CDS             <1..858
                     /locus_tag="VF453_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7688202.1"
                     /translation="QAAAPQVRTQAPGWFRMPLGDFEVTALSDGFFDLPVDKILKQPA
                     ARTDAALARAFLKLPVETSVNGFLVNTGSRLVLIDTGTGGLEGPTAGSLVANLRAAGY
                     APEQVDDVLITHDHGDHAGGLAHDGAAVFPNATIHVGKGDLTHYTDAAAGDKIAFAAA
                     APYVAAHRLQPIEADGEIVPGIRAWGTPGHTPGHTSYVVESKGERLIVTGDLIHVAAV
                     QMDDPTVTVSFDTDERAAESQRLKVFGQAAREGTWIAAAHLQFPGIGHLRADGKGYRF
                     VPVNYLHAR"
     gene            complement(869..1918)
                     /locus_tag="VF453_10870"
     CDS             complement(869..1918)
                     /locus_tag="VF453_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEX7688203.1"
                     /translation="MIAALAAAVPLLASAQDPARCAALRDDAARLACYDGLFGAKAAA
                     PSVPAAASAPELPVRNGAATLAEVAPSASGRGLAAGDYLERFWELDPGDGRDTFVVRT
                     YQPNFVTAHFSSSVNRAPHSPSHPEGGGFPGYRDVEAEVQVSLRAKVLQDFVGHGNLW
                     LAYTQQSLWQVLDGDDSRPFRASDYKPEADYVVPIPQRLGTLAGWRWRLAQVGIAHES
                     NGLSDPLSRSWNYADLATAFTHDDYALQARHFWRLPESGVNDNGDLTRYIGSTELEAD
                     WFPGTDTHQLTLRTSFDSWSRGSLKYVWTHPVFAAKPDGLRWYLQLFSGYGETLLDYN
                     HHQNSVAVGFTLFQL"
     gene            2135..3145
                     /locus_tag="VF453_10875"
     CDS             2135..3145
                     /locus_tag="VF453_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7688204.1"
                     /translation="MHIQYGSDPNGLICGYLFRGGAAGEPIGLEGARAWLRETEGKET
                     TGDFVWLHFDLTDAGAERWLAEQAEIAPAFFDALHEGSRSTRIEDAHDTLVAVVNDVA
                     FEFAFDPSEIATLWVNVGARIAVTARAHPLRSIDRLRRAVKERGVGFRSPIVFLNRLL
                     HDQGDVLVHIVRKATLQVDDVEDKLLVGRLGSKREDLGRLRRVLVRLQRLLAPEPGAL
                     FRLLRLPPPWIGERDLDELRQATEEFSLVLRDLAALQERIKLLQDEIAAQVGEHTNSS
                     VFTLTVVTVLALPINLVAGLLGMNVGGIPLSQDPHGFLWVAAIVGTVTLVAGWLAFRH
                     RE"
BASE COUNT          440 a         1155 c         1176 g          436 t
ORIGIN      
        1 caggccgccg cgccgcaggt ccggacccag gcgcccgggt ggttccgcat gccgctgggc
       61 gacttcgagg tcaccgcgct gagcgacggc ttcttcgacc tgccggtcga caagatcctg
      121 aagcagcccg ccgcccgcac cgacgccgcg ctggccaggg cgttcctgaa gctgccggtc
      181 gagacctcgg tcaacggctt cctcgtcaac acgggctcgc gcctggtgct gatcgacacc
      241 ggcaccggcg gcctcgaggg cccgacggcc ggctcgctgg tggccaacct gcgcgcggcc
      301 ggctatgcgc cggagcaggt cgacgacgtc ctgatcacgc acgaccatgg cgaccacgcg
      361 ggcggtctcg cgcacgacgg cgcggcggtg ttccccaacg cgacgatcca cgtcggcaag
      421 ggcgacctga cgcactacac cgacgccgcc gccggcgaca agatcgcgtt cgccgcggcc
      481 gcgccctacg tcgccgcgca ccggctgcag ccgatcgagg ccgacggcga gatcgtcccc
      541 ggcatccgcg cctggggcac gccgggccac acgcccggcc acaccagcta cgtcgtcgag
      601 agcaagggcg agcgcctgat cgtcacgggc gacctgatcc acgtcgcggc ggtgcagatg
      661 gacgacccga ccgtcaccgt gagcttcgat accgacgagc gcgccgccga gtcgcagcgc
      721 ctgaaggtct tcggccaggc cgcgcgcgag ggcacgtgga tcgccgccgc ccacctgcag
      781 ttccccggca tcgggcacct gcgcgccgac ggcaagggct accgcttcgt gccggtgaac
      841 tacctgcacg cgcgctgaag ggcggctctc agagctggaa cagcgtgaag ccgacggcga
      901 cgctgttctg gtgatggttg tagtccagca gcgtctcgcc gtagccggag aacagctgca
      961 ggtaccagcg caggccgtcc ggcttcgcgg cgaagacggg atgcgtccac acgtacttca
     1021 gcgagccgcg gctccacgag tcgaacgagg tgcgcaaggt cagctggtgg gtgtccgtgc
     1081 cggggaacca gtcggcctcg agctcggtgg atccgatgta gcgagtcagg tcgccgttgt
     1141 cgttgacgcc ggactccggc aggcgccaga agtggcgcgc ctgcagcgcg tagtcgtcgt
     1201 gcgtgaaggc ggtggcgagg tcggcgtagt tccagctgcg cgagagcggg tccgacaggc
     1261 cgttggactc gtgcgcgatg ccgacctgcg cgagccgcca gcgccagccc gcgagcgtgc
     1321 ccagccgctg cggtatgggc acgacgtagt cagcctcggg cttgtagtcg ctggcgcgaa
     1381 aggggcgcga gtcgtcgccg tccagcacct gccagagcga ctgctgcgta tacgcgagcc
     1441 acaggttgcc gtggccgacg aagtcctgca gcaccttcgc gcgcagcgac acctgcacct
     1501 cggcctccac gtcgcggtag ccgggaaagc cgccgccctc gggatgcgag gggctgtgcg
     1561 gcgcgcggtt gacgctggag ctgaagtgcg cggtcacgaa gttcggctgg taggtgcgca
     1621 cgacgaaggt gtcgcggccg tctccggggt cgagttccca gaagcgctcg aggtagtccc
     1681 ctgccgcgag cccgcggccg ctcgcgctgg gcgcgacctc ggcgagcgtc gccgccccgt
     1741 tgcgcacggg cagctccggc gccgaggccg ccgcgggcac ggagggcgcg gcggctttcg
     1801 cgccgaacag gccgtcgtag caggccaggc gcgcggcgtc gtcgcgcagt gcggcgcagc
     1861 gcgcgggatc ctgcgcgctc gcgaggagcg gaacggcggc ggcgagggca gcgatcaagg
     1921 gcgttgcgcg catggaaggg gccggcagtg gagcggacgg gtggagggcc ggcacgcacg
     1981 cgcagtcatg cgtggcgccg tatgcggggc gtgccgaggc aaggcacatg cccggccgtc
     2041 cgctgcgcgc cggccttcac gaggccgtca cgaacccgta cgatcgtggg ccgctcgctg
     2101 ctgtccagcg ccattcccgc cgcccacgcc ccgcatgcac atccagtacg gctccgaccc
     2161 caacggcctc atctgcggct acctgttccg cggcggcgcg gcgggcgagc ccatcggtct
     2221 cgagggcgcg cgcgcgtggc tgcgcgagac ggaaggcaag gagacgaccg gcgacttcgt
     2281 ctggctgcac ttcgacctca ccgacgccgg cgccgagcgc tggctcgccg agcaggcgga
     2341 gatcgcgccg gccttcttcg acgcgctgca cgagggctcg cgttcgacgc gcatcgagga
     2401 cgcgcacgac acgctggtgg ccgtcgtcaa cgacgtcgcc ttcgagttcg cgttcgatcc
     2461 ttccgagatc gcgacgctgt gggtcaacgt cggcgcgcgc atcgcggtga cggcacgcgc
     2521 gcatccgctg cgctcgatcg accgcctgcg ccgcgccgtc aaggagcgcg gcgtaggctt
     2581 ccgctcgccg atcgtcttcc tcaaccgcct gctgcacgac cagggcgacg tcctcgtgca
     2641 catcgtgcgc aaggcgacgc tgcaggtcga cgacgtcgag gacaagctgc tggtcggccg
     2701 cctcggctcc aagcgcgagg acctcggccg cctgcgccgc gtgctggtgc ggctgcagcg
     2761 gctgctcgcg cccgagcccg gcgcgctgtt ccgcctgctg cggctgccgc cgccgtggat
     2821 cggggagcgc gaccttgacg agttgcggca ggccaccgag gagttctccc tcgtgctgcg
     2881 cgacctcgcc gcgctgcagg agcgcatcaa gctgctgcag gacgagatcg cggcgcaggt
     2941 cggcgagcac accaattcga gcgtgttcac gctgaccgtc gtgaccgtgc tcgcgctgcc
     3001 gatcaacctc gtcgccggcc tgctcgggat gaacgtcggc ggtatcccgc tgtcgcagga
     3061 cccgcacggc ttcctctggg tcgccgcgat cgtcgggacg gtgacgctgg tggcgggctg
     3121 gctcgcgttc cggcatcgcg agtagcgtcc cggccccggc ggccccggcg gccccggcgg
     3181 ccccggcggc cccggcggcc ccggcgg
//
LOCUS       DASVLN010000447         3555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_77199, whole genome shotgun sequence.
ACCESSION   DASVLN010000447 DASVLN010000000
VERSION     DASVLN010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77199"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VF453_10880"
     CDS             complement(<1..399)
                     /locus_tag="VF453_10880"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX7688205.1"
                     /translation="MPAAPSAALPLASADAAVPRDEPVSPSSEPVVVSHRFNTLEAEL
                     LRGCLEAHGIPTTLGDVQTIQTDTLWTVALGGVRVMVPESAAEDARRLVAQFDRGELA
                     VDESQFDAAAVPPDAVDDAPAPSSGRALRWA"
     gene            complement(435..827)
                     /locus_tag="VF453_10885"
     CDS             complement(435..827)
                     /locus_tag="VF453_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEX7688206.1"
                     /translation="MIDATETVHQVVATDAALALIERLRGKHGALMFHQSGGCCDGSA
                     PMCYPLGEFLVGDADRLLGEIGGAPFYIGESQYEYWCHTQLVIDVVPGRGGMFSLKGP
                     EGLRFLTRSRFYDDGEWAALVAAGKVER"
     gene            complement(873..2393)
                     /gene="adh"
                     /locus_tag="VF453_10890"
     CDS             complement(873..2393)
                     /gene="adh"
                     /locus_tag="VF453_10890"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX7688207.1"
                     /translation="MDMLEPGRVGFAVDFKKRYGNYIGGEWTAPLKGDYFENVTPVTG
                     RAFCEIPRSGAEDIEAALDAAHAAKSAWGRTSPAERANLLNRIADRMEQNLELLATAE
                     TWDNGKPIRETMAADIPLAIDHFRYFAGAVRAQEGGISEIDETTVAYHFHEPLGVVGQ
                     IIPWNFPILMAVWKLAPALAAGNCVVIKPAEQTPASILVLMELVADLLPKGVVNVVNG
                     FGLEAGKPLASSRRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFFEDVC
                     AKDDAFFDKALEGFAMFALNQGEVCTCPSRALVQESIYDRFMERAIKRVAAIKQGNPL
                     DKSTMIGAQASSEQLEKILSYLDLGRQEGAQCLIGGERNMLKDDLEGGYYVKPTVFKG
                     HNRMRIFQEEIFGPVVSVTTFKDEEDALAIANDTLYGLGAGVWSRDMNTAFRMGRGIQ
                     AGRVWTNCYHAYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKNLLVSYSDKALGFF"
     gene            2588..3388
                     /locus_tag="VF453_10895"
     CDS             2588..3388
                     /locus_tag="VF453_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7688208.1"
                     /translation="MIRLLLFVLAAAALAYAAACAGLYAWQRQLMYFPPPQRTVATRQ
                     VDSFRRDGVLLQLSVRPHPGRGALLYFGGNAEDVSASLAPLAAAFPDREVVALHYRGY
                     GGSEGHPTEAANAADALALFDRVHALHPDVILMGRSLGSGIATRLAAARPASGLVLVT
                     PYDSLLALAQAEFPWFPVRWLLLDRYETWRYVPAVKAPVLILRAGQDEVIPAASTERL
                     AARFPPGRARTVVLPRAGHNNVADDPLYVQSLQAFDRALIPLATERER"
BASE COUNT          467 a         1291 c         1243 g          554 t
ORIGIN      
        1 cgcccagcgc agcgcgcgcc cggacgacgg cgccggcgcg tcgtcgaccg cgtcgggcgg
       61 caccgccgcg gcgtcgaact gcgattcgtc gaccgcgagc tcgccgcgat cgaactgcgc
      121 gaccagccgc cgagcgtcct cggcggcgga ctcgggcacc atcacccgca cgccgccgag
      181 cgcgaccgtc cacagcgtgt cggtctggat ggtctgcacg tcgcccagcg tcgtcgggat
      241 gccgtgggcc tcgaggcagc cgcgcagcag ctcggcctcg agcgtattga accgatggct
      301 gacgaccacc ggctccgagg acggcgacac gggctcgtcg cgcggcaccg ccgcgtccgc
      361 ggaggcgagg ggcagggcgg cggacggcgc ggcgggcatg cggccactgt acgcgaccgc
      421 gcgcggcgtg tcgctcagcg ctcgaccttg cccgccgcca ccagcgccgc ccactcgccg
      481 tcgtcgtaga agcgcgagcg cgtcaggaag cgcaagccct ccggcccctt gagcgagaac
      541 atgccgccgc ggcccggcac gacgtcgatg accagctgcg tgtggcacca gtactcgtac
      601 tgcgactcgc cgatgtagaa cggcgcgccg ccgatctcgc cgagcagccg gtcggcgtcg
      661 ccgaccagga actcgccgag cggatagcac atcggcgcgc tgccgtcgca gcagccgccg
      721 gactggtgga acatcagcgc gccatgcttg ccgcgcaggc gttcgatcag cgccagcgcg
      781 gcgtcggtgg ccacgacctg gtgcacggtt tcagtcgcat cgatcatggc gtggtcctct
      841 tgccggcgct gccgctcatc cgccccgagg gctcaaaaga acccgagcgc cttgtcgctg
      901 taggacacca gcaggttctt cgtctgctgg tagtggtcga gcatcatctt gtggttctcg
      961 cgcccgatgc cggactgctt gtagccgccg aaggccgcgt gcgcgggata ggcgtggtag
     1021 cagttcgtcc acacccggcc ggcctggatg ccgcgaccca tgcggaaggc ggtgttcatg
     1081 tcgcgcgacc acacgcccgc gccgaggccg tacagcgtgt cgttggcgat cgccagcgcg
     1141 tcctcctcgt ccttgaaggt cgtcaccgag accaccggcc cgaagatctc ctcctggaag
     1201 atgcgcatgc ggttgtggcc cttgaacacc gtgggcttga cgtagtagcc gccttcgagg
     1261 tcgtccttga gcatgttgcg ctcgccgccg atcaggcact gcgcgccttc ctgcctgccc
     1321 aggtcgaggt agctcaggat cttctcgagc tgctcgctgg aggcctgcgc gccgatcatc
     1381 gtcgacttgt ccagcggatt gccctgcttg atcgcggcca cgcgcttgat cgcgcgctcc
     1441 atgaagcggt cgtagatcga ctcctgcacc agcgcgcgcg acgggcaggt gcagacctcg
     1501 ccctggttca gcgcgaacat cgcgaagccc tcgagcgcct tgtcgaagaa cgcgtcgtcc
     1561 ttcgcgcaca cgtcctcgaa gaagatgttg ggcgacttgc cgccgagctc cagcgtgacc
     1621 gggatcaggt tctggctcgc gtactgcatg atcagccgcc cggtcgtggt ctcgccggtg
     1681 aacgcgatct tggcgatgcg cctgctcgag gcgagcggct tgcccgcctc caggccgaag
     1741 ccgttgacga cgttgacgac gcccttgggc agcaggtcgg cgacgagctc catcagcacg
     1801 aggatggacg cgggcgtctg ctccgcgggc ttgatcacca cgcagttgcc ggccgcgagc
     1861 gcgggcgcga gcttccacac cgccatcagg atcgggaagt tccacgggat gatctggccg
     1921 accacgccca gcggctcgtg gaaatggtag gcgacggtgg tctcgtcgat ctcgctgatg
     1981 ccgccttcct gggcgcgcac cgcgccggcg aagtagcgga agtggtcgat cgccagcggg
     2041 atgtcggcgg ccatcgtctc gcggatcggc ttgccgttgt cccaggtctc cgcggtcgcg
     2101 agcagctcca ggttctgctc catccggtcg gcgatgcggt tgaggagatt ggcgcgctcg
     2161 gccggcgacg tccggcccca ggcggacttg gcggcgtgcg cggcgtcgag cgcggcctcg
     2221 atgtcctcgg cgcccgagcg cgggatctcg cagaacgcgc ggccggtgac cggcgtgacg
     2281 ttctcgaagt agtcgccctt gagcggcgcc gtccattcgc cgccgatgta gttgccgtag
     2341 cgcttcttga aatccacggc gaagccgacg cggccgggtt ccagcatgtc catggttgtc
     2401 tcctgcgttg agtgacttcc ggcgaaggat ctgccgcgtg ggcccgcgag gcgcggcgcg
     2461 ccgcggcgga cgctgccgtg agcggacgat gcgagcgggc cggcggcctg tcaaggccag
     2521 gggcaagcgt cccgcgcgcg ggggttcaca atgcggctcc cggctcatcc atggtcggga
     2581 ccacgccgtg atccgcctcc tgctcttcgt cctcgccgcc gccgcgctcg cctatgccgc
     2641 cgcctgcgcc ggcctgtacg cgtggcagcg ccagctcatg tatttcccgc cgccgcagcg
     2701 caccgtcgcc acgcggcagg tggacagctt ccgtcgcgac ggtgtcctgc tgcaactgag
     2761 cgtgcgtccg cacccgggcc gcggtgcgct gctctatttc ggcggcaacg cggaggacgt
     2821 gtcggccagc ctcgccccgc tggcggccgc gttccccgac cgcgaggtcg tcgcgctgca
     2881 ctaccgcggc tacggcggca gcgagggcca ccccaccgag gccgcgaacg cggccgacgc
     2941 gctggcgctg ttcgaccggg tgcacgcgct gcatccggac gtcatcctga tgggccgcag
     3001 cctgggcagc gggatcgcca cccggctcgc ggcggcgcgg ccggcatcgg ggctggtgct
     3061 cgtcacgccc tacgacagcc tgctcgcgct cgcccaggcc gagttcccgt ggtttcccgt
     3121 gcgctggctg ctgctcgacc gctacgagac ctggcgctac gtgcccgcgg tgaaggcgcc
     3181 ggtgctgatc ctgcgcgcgg gccaggacga ggtcatcccc gccgccagca ccgaacgcct
     3241 ggccgcgcgc ttcccgcccg gtcgggcgcg cacggtggtg ctgccgcgcg ccggccacaa
     3301 caacgtcgcg gacgatccgc tgtacgtgca gtcgctgcag gccttcgacc gcgcgctgat
     3361 cccgctcgcc acggagcgcg agcgatgacg gcgccggcgc tgcacgcggc cttcctgcgc
     3421 aacgtcaacc tcggccggcc gggctcgccc acgcgcgtgc agttcgaggc cgccttcctc
     3481 gaggccggcg cggcgagcgc cacgtccttc ctcgtcaacg gcacgctggt cttcgcggcg
     3541 ccggcgggca tgcgc
//
LOCUS       DASVLN010000448         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_344826, whole genome shotgun sequence.
ACCESSION   DASVLN010000448 DASVLN010000000
VERSION     DASVLN010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344826"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..893)
                     /locus_tag="VF453_10900"
     CDS             complement(45..893)
                     /locus_tag="VF453_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX7688209.1"
                     /translation="MPRFGVPSALEYFSTLVADDDQFPLLEAAIAIAQDEVPELDVEA
                     VLAEIDALALRLKQRLAADAPALHRIRTLNCYFFDELGFAGNVNDYHDPANSYLPEVL
                     ARRRGIPITLALLYMEIAGQVGLRAEGVSFPGHFLVKLRLAQGEVILDPFTGQSLSRA
                     DLEERLDLVRQASAMRAAFDVPLGLFLQAAGPRDIIGRMLRNLQQIHQGRGDLPRLLA
                     VLDRQVVLEPEDWDTYRLRGDILEGLGRAEEAAFDWGLYLDHTPEAADAEALQRRIRA
                     VRSQRH"
     gene            complement(1063..2616)
                     /gene="murJ"
                     /locus_tag="VF453_10905"
     CDS             complement(1063..2616)
                     /gene="murJ"
                     /locus_tag="VF453_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX7688210.1"
                     /translation="MNLLKAASTVSLLTLGSRVTGLVRDQLIAALFGASAATDAYNVA
                     FRIPNLLRRLFAEGAFSQAFVPLLANTRASEGDETTHRLVDAVATVLAWTLVATCVVG
                     IVGAPAIVWAMASGLKRFDTAVVMTRLMFPYIGFMSMVALSAGVLNTWGRFAIPAATP
                     VLLNVSSVFVAWTFTSTIEAHGYPGVYSLAFGVLLGGVLQAGIQIPALMAIGFLPRVR
                     VTWRGLREAWHHPGVHRVLKQMSPALLGVSVAQVSLLINTQLASHLGAGAVSWLTYAD
                     RLMEFPTGLLGVALGVVLIPQLSAARARGDHDGYSGMIDWGLRLVLLQTLPCAVALLV
                     FAEPLISVLFHYGHFTDADVLRTVQPLRGYGLGLLGIVSVKVLAPGYFAQMDTRTPVR
                     IAVAVLVCTQLMNAVFVPWLGVGGLALSIGLGATINASWLLTGLVRRGVYKPQPGWPA
                     FVARALVAAAALGAGLYWAAHAIDWLDRSHPLLRAAKMAGVLGGVAALYFTVLLALGI
                     NLKSFARKA"
     gene            2978..>4200
                     /locus_tag="VF453_10910"
     CDS             2978..>4200
                     /locus_tag="VF453_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7688211.1"
                     /translation="MNFDELGLAAPILRAVHEAGYTTPTPIQLQAIPAVLKGGDLLAG
                     AQTGTGKTAGFTLPMLHRLLAAGPKKDARGRPAIRALVLTPTRELAAQVEESVRTYGK
                     HTGLTSMVMFGGVGMQPQVDRLRKGVDILVATPGRLLDHHQQKTLDLSHVEIFVLDEA
                     DRMLDMGFIHDIKKVLAVLPAKKQSLLFSATFSDEIKALADRLLDNPGLIEVARRNQT
                     NEMIEQAVHPVSRDRKKQLLSHLIKQGDWHQVLVFTRMKHGANRLAEFLNDDGITAMA
                     IHGNKSQTARTRALAEFKAGTLQVLVATDIAARGIDIDQLPHVVNFELPNVPEDYVHR
                     IGRTGRAGAQGRAVSLVCVDEDLFLRDIEKLIKRQIPKEVVAGFEPDPNERAEPIVLG
                     RTTIGVRGSSSGRGGR"
BASE COUNT          692 a         1512 c         1497 g          499 t
ORIGIN      
        1 aaccgcgcat tgtaggcggc ccggcggcgg cgcccggccg cgcctcagtg gcgctgcgac
       61 cgcaccgccc tgatgcgccg ctgcaacgcc tcggcgtcgg cggcctcggg cgtgtggtcg
      121 aggtacaggc cccagtcgaa cgcggcctcc tcggcgcggc cgaggccctc gaggatgtcg
      181 ccgcgcaggc ggtaggtgtc ccagtcctcg ggctcgagca ccacctggcg gtcgaggacg
      241 gccagcaggc gcggcaggtc gccgcggccc tggtggatct gctgcaggtt gcgaagcatg
      301 cggccgatga tgtcgcgcgg ccccgcggcc tgcaggaaca ggccgagcgg cacgtcgaag
      361 gcggcgcgca tcgcgctcgc ctggcggacg aggtcgagcc gctcctccag gtcggcgcgc
      421 gacagcgact gcccggtgaa cggatcgagg atcacctcgc cctgcgccag ccgcagcttg
      481 accaggaaat gccccgggaa ggacacgccc tccgcgcgca ggccgacctg gcccgcgatc
      541 tccatgtaga gcagcgccag cgtgatgggg atgccgcgcc ggcgggcgag cacctccggc
      601 aggtagctgt tggcggggtc gtggtagtcg ttgacgttgc cggcgaagcc gagctcgtcg
      661 aagaagtagc agttgagggt gcggatgcgg tgcagcgccg gcgcgtcggc ggccaggcgc
      721 tgcttcaggc gcagcgcgag ggcgtcgatc tcggccagca cggcctcgac gtcgagctcg
      781 ggcacctcgt cctgggcgat ggcgatcgcg gcctcgagca gcgggaactg gtcgtcgtcg
      841 gcgaccagcg tgctgaagta ctccagcgcg ctcgggacgc cgaagcgggg cacgggcgga
      901 ccgccgccgc cacgacggga gcgcgacggg cgcggggagc tcggaacgga ggccatgcga
      961 accagtgtag acccgccggc gacgccatgc atgcggtcgc gcagggggct ttcccggcgg
     1021 acgcgcggga cgaggcgggg tcggccgcgc gtcggacgct tctcaggcct tgcgggcgaa
     1081 ggacttcagg ttgatcccca gcgccagcag caccgtgaag tagagggccg cgaccccgcc
     1141 gagcacgccg gccatcttcg ccgcccgcag cagcggatgg ctgcggtcga gccagtcgat
     1201 ggcatgcgcg gcccagtaca gcccggcgcc gagcgccgcg gcggccacca gcgcccgcgc
     1261 gacgaacgcc ggccagccgg gctgcggctt gtacacgccg cgccgcacga ggccggtcag
     1321 cagccagctc gcgttgatcg tcgcgcccag cccgatcgac agcgccaggc cgccgacgcc
     1381 cagccacggc acgaacaccg cgttcatcag ctgcgtgcac acgagcaccg cgacggcgat
     1441 gcgcaccggc gtgcgcgtgt ccatctgggc gaagtagccg ggcgcgagca ccttgacgct
     1501 cacgatgccc agcagcccga ggccgtagcc gcgcaggggc tgcacggtgc gcagcacgtc
     1561 ggcgtcggtg aagtggccgt agtggaacag caccgagatc agcggctccg cgaagaccag
     1621 cagggccacc gcgcacggca gcgtctgcag cagcacgagg cgcaggcccc agtcgatcat
     1681 gccggagtag ccgtcgtggt cgccgcgcgc gcgcgccgcc gacagctgcg ggatcagcac
     1741 cacgcccagc gcgacgccca gcaggccggt cgggaactcc atcagccggt cggcgtaggt
     1801 gagccaggac accgcgcccg cgcccaggtg cgacgcgagc tgcgtgttga tcaggagcga
     1861 cacctgcgcg accgagacgc ccagcagcgc cggcgacatc tgcttgagca cccggtgcac
     1921 gcccgggtgg tgccaggcct cgcgcaggcc gcgccaggtc acgcgcaccc gcggcaggaa
     1981 gccgatggcc atcagcgccg ggatctggat gccggcctgc aggacgccgc ccagcagcac
     2041 gccgaacgcg agggagtaca cccctgggta tccatgcgcc tcgatggtgg aggtgaacgt
     2101 ccaggccacg aacaccgacg agacgttgag caggacgggc gtggccgcgg ggatcgcgaa
     2161 ccggccccag gtgttgagca cgccggcaga cagcgccacc atcgacatga agccgatgta
     2221 ggggaacatc agccgcgtca tgacgacggc ggtgtcgaag cgcttcaggc ccgaggccat
     2281 cgcccacacg atcgcgggcg cgccgacgat gccgaccacg caggtggcca cgagcgtcca
     2341 ggccagcacg gtggcgaccg cgtcgacgag gcggtgcgtc gtctcgtcgc cctcggacgc
     2401 gcgcgtgttg gccagcaaag gcacgaaggc ctgcgagaac gcgccctcgg cgaacagccg
     2461 gcgcagcagg ttggggatgc ggaacgcgac gttgtaggcg tcggtggcgg cactggcgcc
     2521 gaacagcgcc gcgatcagct ggtcgcggac gagccccgtg acgcgcgagc ccagcgtgag
     2581 cagcgaaacc gtcgaggcgg ccttgaggag gttcatggat gccgcggcgg ctccggtttc
     2641 ctggaacgcg ttccaggcgg cggcgcaccg ggtgggtgga caggggcgcg attataggaa
     2701 gtccgcgtgc ccccccccgc ggcggcgcga tccggaggcg aaaggtcggc caatcccctt
     2761 tttcggagaa ccaggtcggt ggcaaccctt acaatgtcgc cagccgcttc gagttccagt
     2821 ccagcagcgg tcgtcgtccc tcgacgcaac cctcgacaac actccggtct cggcctccac
     2881 gactggtgcc cgcatgaacc aggccttcgc gagaaggcgc gcggcgcagc aggccgaatc
     2941 cgcgaacccg gctcccgccg ggactgacca agaatccatg aacttcgacg aactcggcct
     3001 ggccgcgccc atcctgcgtg cggtccacga ggccggctac acgaccccga ccccgatcca
     3061 gctccaggcc atcccggccg tcctgaaggg cggcgacctg ctcgccggcg cgcagaccgg
     3121 caccggcaag accgccggct tcacgctgcc gatgctgcac cgcctgctcg ccgcgggccc
     3181 gaagaaggac gcccgcggcc gcccggcgat ccgcgcgctc gtcctcacgc cgacgcgcga
     3241 gctcgccgcg caggtcgagg agagcgtgcg cacctacggc aagcacaccg gcctcacgag
     3301 catggtgatg ttcggcggcg tgggcatgca gccgcaggtc gaccgcctgc gcaagggcgt
     3361 cgacatcctc gtggccacgc ccggccgcct gctcgaccac caccagcaga agacgctgga
     3421 cctgtcgcac gtggagatct tcgtcctcga cgaggccgac cgcatgctgg acatgggctt
     3481 catccacgac atcaagaagg tgctcgcggt gctgccggcg aagaagcaga gcctgctgtt
     3541 ctcggccacc ttcagcgacg agatcaaggc cttggccgat cgcctgctgg acaacccggg
     3601 cctgatcgag gtcgcgcgcc gcaaccagac caacgagatg atcgagcagg ccgtgcaccc
     3661 ggtctcgcgc gaccgcaaga agcagctgct gtcccacctg atcaagcagg gcgactggca
     3721 ccaggtgctc gtgttcacgc gcatgaagca cggcgccaac cgcctggccg agttcctgaa
     3781 cgacgacggc atcacggcga tggcgatcca cggcaacaag agccagaccg cccgcacgcg
     3841 cgcgctggcc gagttcaagg ccggcacgct gcaggtgctg gtggccaccg acatcgccgc
     3901 gcgcggcatc gacatcgacc agctgccgca cgtcgtcaac ttcgagctgc ccaacgtgcc
     3961 cgaggactac gtgcaccgca tcggccgcac cggccgcgcc ggcgcgcagg gccgcgcggt
     4021 ctcgctcgtg tgcgtggacg aggacctttt cctgcgcgac atcgagaagc tgatcaagcg
     4081 ccagatcccc aaggaagtcg tcgccggctt cgagcccgac ccgaacgagc gcgccgagcc
     4141 catcgtcctc ggccgcacca ccatcggcgt gcgcggctcc tcgtccggcc gaggcggccg
//
LOCUS       DASVLN010000449         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_508195, whole genome shotgun sequence.
ACCESSION   DASVLN010000449 DASVLN010000000
VERSION     DASVLN010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508195"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VF453_10915"
     CDS             <1..778
                     /locus_tag="VF453_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7688212.1"
                     /translation="DIADVDPLAPRYKEWDARAAQYQAQARPVAATLPLGGDRLGRDV
                     LAKALKGTQISVSVGVLAAIVATLVGATLGAVAGFFGGWAGDVIEWGYNVVTAVPDIL
                     LFFAFAAVFGRGISTLVLILGLTGWTSPYRQVRAEFLKHARRDYVRAAEAIGASAGAH
                     MFRHILPNASHVILVRMSLLVVGFIKIEVILSYLGLGVATDQVSWGTMLAEAQSDLIL
                     GHWWQLAAATVFMAVFVTAFSLMADAWRDALDPKLRGTLQ"
     gene            775..2499
                     /locus_tag="VF453_10920"
     CDS             775..2499
                     /locus_tag="VF453_10920"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEX7688213.1"
                     /translation="MRTPLLKVEDLRVAFRLHDADGRARRVEAVGRGAQGVSFDVPEN
                     TTVALVGESGSGKSVTAMAVVQLLPRNAECAGRVLWDGDDLLAAPRARMQRLRGREIA
                     CVFQDPVSSLNPVATIGAQIAEPLVKHLGLGRAAARQRAEELLAEVGIPEARRRLDTF
                     PHQLSGGQQQRVMIAMALACEPRLLIADEPTTALDVTIQRQILDLLARVREQRGLSML
                     FISHDLGVVGEIAQQIVVMRAGTVRESGSADALFDAPRDAYTRALLACRPRLDERPRR
                     LAVVDAVPDPRDAQPAVPRSRDADLDDPVVLQVDRLSKSFWIRTGVLRRSEFRAVHDV
                     SLTLRRGHTLGVVGESGSGKSTMGLTLLRLHDASSGPLAGRVLFHGHDLLALAPADRL
                     AMRRRIQIVFQNPYEALNPRFTIGQTLVEPMAIHRIGASPAERLAKARDWLVRVGLDA
                     DALHRYPHEFSGGQRQRIAIARSLTLSPDVLVLDEAVSALDVSVQAQVLNLLKDLQDE
                     LGLAYLFISHDFAVVRFMADDVLVMKDGRVVEHRPTEALLAQPQDAYTRRLIGAVPSG
                     RPARAATA"
     gene            complement(2500..>3370)
                     /locus_tag="VF453_10925"
     CDS             complement(2500..>3370)
                     /locus_tag="VF453_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688214.1"
                     /translation="YPDARRRGAFDELNAARQGAGAGLLAQDAALDTTTGDHVAYLGL
                     NGYGSAGSPHDETPGLPGYTGTTPFTRMTAAGYAFSFAGEVIGDIGSSSATTDCVGDL
                     LDTIYHAIDMLGHETDVGIAFGSGATAGMCTIDMAKPLTDGARQIPPSGAVVTYPYAG
                     QTVASGTFRVANESPRAPVSLLPSPTAGTPILAGLRNQDFLVSHAATVSAFTLTNASG
                     AAVPAVILAGSGISGPDVNADPNIDADSGFVVLVPTSPLPAGRYTATLRATVAGGRAL
                     ATSTWSFSVASQQ"
BASE COUNT          416 a         1220 c         1263 g          471 t
ORIGIN      
        1 cgacatcgcc gacgtcgacc cgctcgcccc gcgctacaag gaatgggacg cccgcgccgc
       61 gcagtaccag gcgcaggcgc ggccggtcgc cgcgacgctg cccctgggcg gcgaccgcct
      121 cggccgcgac gtgctcgcca aggcgctcaa gggcacgcag atctcggtct cggtgggcgt
      181 gctcgccgcg atcgtcgcga cgctcgtggg cgccacgctc ggcgcggtcg cgggcttctt
      241 cggcggctgg gccggcgacg tcatcgagtg gggctacaac gtcgtcaccg cggtgcccga
      301 catcctgctg ttcttcgcgt tcgcggcggt gttcgggcgg gggatctcga cgctcgtgct
      361 gatcctcggc ctcaccggct ggacgagccc ctaccgccag gtgcgcgcgg agttcctcaa
      421 gcacgcgcgc cgcgactacg tgcgcgccgc cgaggcgatc ggcgcgagcg ccggcgcgca
      481 catgttccgc cacatcctgc ccaacgcgag ccacgtgatc ctcgtgcgga tgtcgctgct
      541 ggtcgtgggc ttcatcaaga tcgaggtgat cctgtcgtac ctcggcctgg gcgtggccac
      601 cgaccaggtg agctggggca cgatgctcgc cgaggcgcag agcgacctga tcctcggcca
      661 ctggtggcag ctcgccgcgg cgacggtgtt catggcggtg ttcgtgaccg cgttctcgct
      721 gatggccgac gcctggcgcg acgcgctcga tccgaagctg cgggggacgc tccaatgagg
      781 acgccgctgc tgaaggtcga ggacctgcgc gtcgcgttcc gcctgcacga cgccgacggc
      841 cgcgcgcgcc gcgtcgaggc cgtgggccgc ggcgcgcagg gcgtgagctt cgacgtgccg
      901 gagaacacga ccgtcgcgct ggtgggcgag tcgggctcgg gcaagagcgt gacggcgatg
      961 gccgtggtgc agctgctgcc gcgcaacgcc gagtgcgcgg gccgcgtgct gtgggacggc
     1021 gacgacctgc tcgcggcgcc gcgcgcgcgc atgcagcgcc tgcgcgggcg cgagatcgca
     1081 tgcgtgttcc aggatccggt gtcctcgctg aacccggtcg cgacgatcgg ggcgcagatc
     1141 gccgagccgc tggtcaagca cctgggcctc ggccgcgccg ccgcgcgcca gcgcgccgag
     1201 gagctgctcg ccgaggtcgg cattcccgag gcacggcgcc gcctcgacac cttcccccac
     1261 cagctctcgg gcggccagca gcagcgcgtg atgatcgcga tggcgctcgc ctgcgagccg
     1321 cggctgctga tcgccgacga gcccaccacc gcgctggacg tgacgatcca gcgccagatc
     1381 ctcgacctgc tcgcccgcgt gcgcgagcag cgcgggctgt cgatgctgtt catcagccac
     1441 gacctcggcg tcgtcggcga gatcgcgcag cagatcgtcg tgatgcgcgc gggcaccgtg
     1501 cgcgagtcgg gctcggccga cgcgctgttc gacgcgccgc gcgacgccta cacccgcgcg
     1561 ctgctcgcct gccggccgcg gctggacgag cgcccgcgcc gcctcgcggt cgtcgacgcc
     1621 gtgcccgatc cgcgcgacgc gcagccggcg gtgccgcgct cgcgcgacgc cgatctcgac
     1681 gacccggtcg tgctgcaggt cgaccgcctc agcaagtcgt tctggatccg cacgggcgtc
     1741 ctgcgccgca gcgagttccg cgcggtgcac gacgtgagcc tcacgctgcg ccgcggccat
     1801 acgctgggcg tggtcggcga gtcgggctcg ggcaagtcga cgatgggcct gacgctgctg
     1861 cgcctgcacg acgccagcag cggcccgctg gccgggcgcg tgctgttcca cggccacgac
     1921 ctgctcgcgc tcgcgccggc cgaccggctc gcgatgcggc gccgcatcca gatcgtgttc
     1981 cagaacccgt acgaggcgct gaacccgcgc ttcacgatcg ggcagacgct ggtcgagccg
     2041 atggcgatcc accgcatcgg cgcctcgccc gccgaacggc tcgcgaaggc gcgcgactgg
     2101 ctcgtgcgcg tgggcctgga tgccgacgcc ctgcaccgct atccccacga gttctcgggc
     2161 ggccagcgcc agcgcatcgc gatcgcgcgc agcctgaccc tgtcgccgga cgtgctggtc
     2221 ctggacgagg cggtctcggc gctcgacgtc tccgtccagg cccaggtgct gaacctgttg
     2281 aaggacctgc aggacgagct gggcctcgcc tacctgttca tcagccacga cttcgcggtc
     2341 gtgcgcttca tggccgacga cgtgctcgtg atgaaggacg gccgcgtcgt cgagcaccgt
     2401 cccaccgagg cgctgctcgc gcagccgcag gacgcctaca cgcgccgcct catcggcgcc
     2461 gtgcccagcg gtcgtccggc gcgcgcggcg acggcctgat cactgctgcg aggcgacgga
     2521 gaacgaccag gtcgaggtcg ccagcgcgcg tccgcccgcg acggtggcgc gcagcgtcgc
     2581 ggtgtagcgg cccgcgggca gcgggctcgt cggcacgagc acgacgaagc cggagtcggc
     2641 gtcgatgttc ggatccgcgt tcacgtcggg gccgctgatg cccgagccgg ccaggatcac
     2701 cgccggcacc gcggcgccgc tcgcgttcgt gagcgtgaac gcggagaccg tcgcggcgtg
     2761 cgagacgagg aagtcctggt tgcgcaggcc ggcgaggatc ggcgtgcccg cggtcgggga
     2821 cggcagcagc gagaccgggg cgcgcgggct ctcgttggcg acgcggaagg tgccgctcgc
     2881 cacggtctgg ccggcgtagg gataggtcac gaccgcgccg gagggcggga tctgccgcgc
     2941 gccgtccgtc agcggcttgg ccatgtcgat cgtgcacatg cccgcggtcg cgccgctgcc
     3001 gaaggcgatg ccgacgtcgg tctcgtggcc gagcatgtcg atcgcatggt agatcgtgtc
     3061 gagcaggtcg cccacgcagt cggtggtggc ggagctggag ccgatgtcgc cgatcacctc
     3121 gcccgcgaac gagaacgcgt agccggccgc ggtcatgcgc gtgaacggcg tcgtgcccgt
     3181 gtagccgggc aggcccgggg tctcgtcgtg cggcgagccg gcgctgccgt agccgttgag
     3241 cccgaggtag gccacgtggt cgccggtggt cgtgtcgagc gccgcgtcct gcgccagcag
     3301 gcccgcgccg gcgccctggc gggcggcgtt gagctcgtcg aaggcgccgc ggcggcgggc
     3361 gtccggatag
//
LOCUS       DASVLN010000450         1866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_184667, whole genome shotgun sequence.
ACCESSION   DASVLN010000450 DASVLN010000000
VERSION     DASVLN010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184667"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VF453_10930"
     CDS             complement(<1..264)
                     /locus_tag="VF453_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688215.1"
                     /translation="MTDDADRPGHPRRNDETHAPAPAPVPAHEEDDGESTLMLPPEKL
                     AEMMAAAAAAVAASTSIQAPPVPPVSPVAPPVPPVAPAPVPVPV"
     gene            complement(341..>1866)
                     /locus_tag="VF453_10935"
     CDS             complement(341..>1866)
                     /locus_tag="VF453_10935"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7688216.1"
                     /translation="VDARINRGNALHALQRHAEAVADYDDALRARPVAGVASNRGDAL
                     LAAGRYPEAVAAFEQALALDPELPWLPGKVLHARMMACDWRDDLPALLARIDAGVAAG
                     RPVIEPFAYNGVATSAEALRRCAESFAADQFPPRPARVPAGHRYGHAKLRIGYLCGEF
                     RHQATSILAVELWELHDKSRFELFAFDNGWDDGSPLRRRIVAAFDEMVDISALSDDAA
                     ADAVVRREIDVLVNLNGWFGRGRTGVFARRPAPVQVNFLGFPGTIGAPYVDAIVADAH
                     VIPPGEEGGYVERVVRLPGCYQPNDRRREIAPVAPTRAELGLPAEAVVLACFNNAYKI
                     LPATFAAWMRVLAAVPDAVLWLLQDTDAASANLRAAAAAHGVDPARLHFAPRAPLPEH
                     LARHARADLFLDTLPYNAHTTASDALWAGLPVLSCAGTTFPGRVGASLLHAVGLQDEL
                     LATGEDDYVARAIALARDRAALARLRETLARRRDTSPLFDTPAYARHLEDAFVRLSS"
BASE COUNT          244 a          658 c          740 g          224 t
ORIGIN      
        1 cacgggcacg ggcacgggcg cgggcgcgac cgggggtacc ggcggggcca ccggcgacac
       61 gggcggcacc ggtggcgcct ggatcgaagt cgacgccgcg accgccgcgg cggcggcggc
      121 catcatctcg gcgagcttct cgggcggcag catcagcgtg gattcgccgt cgtcctcctc
      181 gtgcgcgggc acgggcgccg gcgcgggggc gtgggtctcg tcgttccggc ggggatgccc
      241 cgggcggtct gcgtcgtcgg tcaaagtcgt ctccgtcttt caagaaggcc accggacgtc
      301 gagctcatcc gtgcggcagc ccggcagttg gcgtgccagc tcatgaggac agccggacga
      361 aggcgtcctc gaggtggcga gcataggccg gcgtgtcgaa cagcggtgaa gtgtcgcgcc
      421 gtcgcgccag cgtctcgcgc aggcgcgcca gcgcggcgcg gtcgcgggcg agcgcgatgg
      481 cgcgcgccac gtagtcgtcc tcgcccgtgg cgagcagctc gtcctgcagc cccaccgcgt
      541 gcagcaggct cgcgccgacg cgacccggga acgtggtgcc ggcgcacgag agcaccggca
      601 gcccggccca cagcgcgtcg ctggcggtgg tgtgggcgtt gtagggcagc gtgtcgagga
      661 acaggtcggc ccgcgcgtgg cgggcgaggt gctcgggcag gggcgcgcgc ggcgcgaaat
      721 gcaggcgtgc gggatcgacg ccgtgcgccg ccgccgcggc gcgcaggttg gcggacgcgg
      781 cgtcggtgtc ctgcagcagc cagagcaccg cgtcgggcac ggccgcgagc acgcgcatcc
      841 acgccgcgaa agtcgccggg aggatcttgt acgcgttgtt gaagcaggcg aggacgaccg
      901 cctccgccgg cagcccgagc tcggcgcggg tcggagcgac gggggcgatc tcgcgccggc
      961 ggtcgttggg ctggtagcag ccgggcagcc gcacgacgcg ctcgacgtag ccgccctcct
     1021 cgcccggcgg gatcacgtgg gcgtccgcga cgatggcgtc gacgtagggc gcgccgatcg
     1081 tccccgggaa gccgaggaag ttgacctgca ccggcgccgg ccggcgcgcg aacacgccgg
     1141 tgcgcccgcg cccgaaccag ccgttgagat tgacgaggac gtcgatctcg cgccgcacga
     1201 cggcgtccgc ggcggcgtcg tcggacagcg ccgagatgtc caccatctcg tcgaacgccg
     1261 cgacgatgcg ccggcgcagc gggctgccgt cgtcccagcc gttgtcgaac gcgaacagct
     1321 cgaagcgcga cttgtcgtgc agctcccaca gctccacggc gaggatggac gtggcctggt
     1381 ggcggaactc gccgcacagg tagccgatgc gcagcttcgc gtggccgtag cgatggcccg
     1441 cggggacgcg cgccggtcgc ggcgggaact ggtcggcggc gaaggattcg gcacagcggc
     1501 gcagcgcctc ggccgaggtc gcgacgccgt tgtacgcgaa cggctcgatc accggccggc
     1561 ccgcggccac gcccgcatcg atgcgcgcca gcagcgccgg caggtcgtcg cgccagtcgc
     1621 aggccatcat gcgcgcgtgc agcaccttgc cgggcagcca cggcagctcg gggtcgagcg
     1681 cgagcgcctg ctcgaacgcc gcgaccgcct ccggatagcg tccggcggcc agcagcgcgt
     1741 cgccgcggtt ggaggcgacg cccgcgacgg ggcgcgcgcg cagcgcgtcg tcgtagtccg
     1801 caacggcctc cgcgtggcgt tgcagcgcat gcagcgcatt gccgcggttg atgcgcgcgt
     1861 ccacgt
//
LOCUS       DASVLN010000451         1792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_345042, whole genome shotgun sequence.
ACCESSION   DASVLN010000451 DASVLN010000000
VERSION     DASVLN010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345042"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1488
                     /gene="argG"
                     /locus_tag="VF453_10940"
     CDS             154..1488
                     /gene="argG"
                     /locus_tag="VF453_10940"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX7688217.1"
                     /translation="MTTILQHLPAGQKVGIAFSGGLDTSAALHWMRLKGAIPYAYTAN
                     LGQPDEPDYDEIPRKAMQYGAEKARLIDCRPQLVAEGIAALQSGAFHISTAGVTYFNT
                     TPLGRAVTGTMLVAAMKEDDVHIWGDGSTFKGNDIERFYRYGLLANPSLKIYKPWLDQ
                     VFIDELGGRAEMSEFLNKAGFGYKMSAEKAYSTDSNMLGATHEAKDLEQLDSGIKIVQ
                     PIMGVAFWREDVPVKAEEVRVRFHEGVPVALNGVEYTDQVALLTEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLFAAYERLVTGIHNEDTIAQYRDNGRALGRLLYQG
                     RWFDSQALMLRETAQRWVARAITGEVTLELRRGNDYSILNTESPNLTYKPERLTMEKG
                     ELSFTPADRIGQLTMRNLDIQDTREKLMTYARTGLLGAGTGAEMLKLGGKDE"
BASE COUNT          306 a          660 c          597 g          229 t
ORIGIN      
        1 cggttcggca tctgcacccc gtcggccggc gcgccggatt cgcgagcgat ccgcggcccg
       61 gccacccgcc ggcgcgctgg cccgcggcct ccgaacaggc gagaatggcg gggtttgtac
      121 cgatccgccc cgcccccgaa cgacgtcgcg tccatgacca ccatcctcca gcacctgccc
      181 gccggccaga aggtcggcat cgccttctcc ggcggcctcg acaccagcgc cgccctgcac
      241 tggatgcgcc tgaagggcgc gatcccctac gcgtacacgg ccaacctcgg gcagcccgac
      301 gagccggact acgacgagat cccgcgcaag gcgatgcagt atggcgccga gaaggcgcgc
      361 ctgatcgact gccggccgca gctcgtggcc gagggcatcg ccgcgctcca gagcggcgcg
      421 ttccacatct cgacggccgg cgtcacctac ttcaacacga cgccgctggg ccgggccgtc
      481 accggcacga tgctggtggc cgcgatgaag gaggacgacg tccacatctg gggcgacggc
      541 agtaccttca agggcaacga catcgagcgc ttctaccgct acggcctgct cgccaacccg
      601 tcgctgaaga tctacaagcc ctggctcgac caggtcttca tcgacgagct cggcggtcgc
      661 gcggagatgt cggagttcct caacaaggcg ggcttcggct acaagatgtc cgcggagaag
      721 gcgtattcga ccgactccaa catgctgggg gcgacgcacg aggccaagga cctcgagcag
      781 ctggattccg gcatcaagat cgtccagccc atcatgggcg tggcgttctg gcgcgaggac
      841 gtccccgtca aggccgagga ggtgcgcgtg cgcttccacg agggcgtgcc ggtcgcgctc
      901 aacggcgtcg agtacaccga ccaggtggcg ctcctgaccg aggccaaccg catcggcggc
      961 cgccacggcc tgggcatgag cgaccagatc gagaaccgca tcatcgaggc caagagccgc
     1021 ggcatctacg aggcgccggg cctcgcgctg ctgttcgccg cctacgagcg cctggtcacc
     1081 ggcatccaca acgaggacac gatcgcccag taccgcgaca acgggcgcgc gctcggccgc
     1141 ctgctgtacc agggccgctg gttcgactcg caggcgctga tgctgcgcga gaccgcccag
     1201 cgctgggtcg cgcgcgcgat caccggcgag gtgacgctgg agctgcgccg cggcaacgac
     1261 tactcgatcc tgaacaccga gtcgcccaac ctcacgtaca agcccgagcg cctgacgatg
     1321 gaaaagggcg agctgagctt cacccccgcg gaccgcatcg gccagctgac catgcgcaac
     1381 ctggacatcc aggacacgcg cgagaagctg atgacctacg cccgcaccgg gctgctgggt
     1441 gcaggcaccg gggcggagat gctgaagctg ggcggcaagg acgagtgagc aactcgcgcc
     1501 cctgacccgc caggacggcc cgccttgcgc gggccgtctt cattccaggg cctcgggcat
     1561 cgccggaccg agcgccgagc gcagcaggtc gaaaggcgcc cttgcacgaa tttgaggcgg
     1621 cgccgcgcgg tgctgtcgaa gcgttgccgg ccaggaacga aacttgatgg gcaccccgcc
     1681 gtgcaggcgt cgccgcgcag cgcccggccg gcaacgccga ccctgcgagg agaccccgat
     1741 gccgcgccgc cgatccgccc tgcccgctcc gctcgccctg gccgcgctgc tc
//
LOCUS       DASVLN010000452         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_398524, whole genome shotgun sequence.
ACCESSION   DASVLN010000452 DASVLN010000000
VERSION     DASVLN010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398524"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VF453_10945"
     CDS             complement(<1..165)
                     /locus_tag="VF453_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7688218.1"
                     /translation="MKRRPRIYYSEAQKAEMWARWKEGWSLQQIAQLFDRSHSSVQGI
                     LAESGGIPPPQ"
     gene            complement(314..499)
                     /locus_tag="VF453_10950"
     CDS             complement(314..499)
                     /locus_tag="VF453_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688219.1"
                     /translation="MNQFFARTREFIAESTSLDAGLNSPCSAMNSFLCEGNCFCFEVI
                     ELPVEVISDGRRSGSRV"
     gene            complement(1185..>2298)
                     /locus_tag="VF453_10955"
     CDS             complement(1185..>2298)
                     /locus_tag="VF453_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEX7688220.1"
                     /translation="GSTAGPLSGSPPPPPPSPPPPPPPPPPAPARLTLDGATIRDPTG
                     AAITLRGINEGTWGEMQPQDAGIIAANGANVVRMLIRWWGLYGGSDVESRDDADPGHF
                     LPDHVAALLQQVQRCIDAGLWVVVAIDSNCGQNGLQDAPTAAYCDPAGAFPAGHNFWT
                     DLTQRALFQDAWVYLAGLLKDMPNIAMYELLPEPLGHHDASWAPEVSAFYQQLMAAIE
                     SDAGDTRTPFLVGARNGYDIRACDEAYIADPHWADRVVYTGDLFAHTDAGTPAQVLAD
                     LQSRFDALVAMRDARAVPVFVQQFGVRTGDDPDETILSGGLAMMDAAGVGYTGWQWRQ
                     NTTDPSQFAVVVADPVTGADVYKTAVLQAYSAGWKA"
BASE COUNT          380 a          779 c          801 g          338 t
ORIGIN      
        1 ctgtggcggc gggatgccgc cgctttcagc cagaatgcct tgcactgacg agtgggaccg
       61 atcgaacagc tgggcgatct gctgcaacga ccacccctcc ttccagcgtg cccacatctc
      121 ggccttctgg gcctccgagt agtagatccg aggtcgccgc ttcatcgcaa cactccttcc
      181 gccaagtcag gcggtcatag tgtgttgcat cgaccggttg aatccgccag cgaaagcggt
      241 cgtcggacac ggtgacgcgg aggcctggag ccctcggcat tcctgacgtt ggcgcaggcc
      301 gcttcaagct gcctcacacc cgcgacccac tgcggcggcc gtccgatatc acctccacgg
      361 gcagttcaat cacctcgaaa cagaaacagt tcccctcgca gaggaatgag ttcatcgcgg
      421 aacatggcga attcaatccg gcgtccaatg aagtgctctc ggcgatgaat tcgcgggttc
      481 gcgcgaagaa ctgattcatc gtcccattga acccaatcct ctcgggatcg aattggttca
      541 atcgccgaca tagttgacgc accggaacgg tgcgacaact ctatcgggag atgaaatggt
      601 tcaatccgga ggggaatcga cgagctcccg gaagagcccg gttggtctcc gaggggacgg
      661 ggcgagttgc gcgagcgcgt cgatgaagtc gcccgaccag cgggacggcc ccccaccgcc
      721 gaagcgccgg catgctcctg ctgccgaggc cctcgatggg ccgaggcacg cccactcttc
      781 ccacagaaga agccgcggcg acaggccggg caggtgtctt ggatcgcagg cgggtcggca
      841 cgtcgcgggc atggtggaac cgaaggagta ctcccaggac gcggccaggc aggcattgcc
      901 ggtcgcgaat cttcaccgaa cgcccacgcg ggaaatcggc cgtccaggcc gatttccaga
      961 tgcacgagag ccggagagcc aagacacctg cccggcctgt cgccgccggc ccgctgacca
     1021 cgccccaccc aggcgcgccg cgccgccgac caccgaccac acccacgcac caggcgcgcc
     1081 gtggaagatc atccagccca cgtccggcag gtcacgacga agtccgagcc ctccgacgtc
     1141 cgagccgccg cgccgcagca tgccagaccg cggcaacccg gccctcacgc cttccatcca
     1201 gccgaatagg cctgcaacac cgccgtcttg tagacatccg cccccgtgac cggatccgcc
     1261 accaccaccg cgaactgcga cggatcggtg gtgttctgcc gccattgcca gccggtgtag
     1321 ccgacgcccg cggcgtccat catcgcgagg ccgccggaca ggatggtctc gtcggggtcg
     1381 tcgccggtgc gcacgccgaa ctgctggacg aagacgggga cggcgcgggc gtctcgcatc
     1441 gcgacgaggg cgtcgaagcg cgattgcagg tcggcgagca cctgggccgg cgttccggcg
     1501 tcggtgtggg cgaagaggtc gccggtgtag acgacgcggt cggcccagtg cgggtcggcg
     1561 atgtaggctt cgtcgcaggc gcggatgtcg tagccgttgc gcgccccgac gaggaagggc
     1621 gtgcgggtgt cgccggcgtc ggactcgatc gcggccatga gctgctggta gaaggccgag
     1681 acctcgggcg cccagctcgc gtcgtggtgg ccgagcggct cgggcagcag ctcgtacatc
     1741 gcgatgttgg gcatgtcctt gaggagtccg gcgaggtaga cccaggcgtc ctggaacagg
     1801 gcgcgctgcg tgaggtcggt ccagaagttg tggccggcgg gaaaggcgcc ggcggggtcg
     1861 cagtaggcgg cggtcggcgc gtcctgcagg ccgttctggc cgcagttgga gtcgatcgcc
     1921 acgaccaccc acagcccggc gtcgatgcac cgctgcacct gctgcagcag cgcggcgaca
     1981 tggtcgggca ggaaatgccc gggatcggca tcgtcgcggc tctcgacgtc gctgccgccg
     2041 tacaggcccc accagcggat cagcatgcgc acgacgttgg cgccgttggc cgcgatgatg
     2101 cccgcgtcct gcggctgcat ctccccccag gtgccctcgt tgatgccgcg cagcgtgatc
     2161 gcggcgccgg tcgggtcgcg tatcgtcgcg ccgtccagcg tcaggcgcgc gggcgcggga
     2221 ggcggcggtg gtggaggtgg cggcggggat ggcggcggtg gcggcggcga cccggacagc
     2281 gggccggccg tcgagccg
//
LOCUS       DASVLN010000453         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_184687, whole genome shotgun sequence.
ACCESSION   DASVLN010000453 DASVLN010000000
VERSION     DASVLN010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184687"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VF453_10960"
     CDS             <1..711
                     /locus_tag="VF453_10960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7688221.1"
                     /translation="MPFPSEPAADHPLDNVMWSALTTRQAPLARGQGLARRFDPAYAR
                     FAGVAEFSPAALDALADLLEPGEDVSVFPPDDALDPGPRFEVLYRKPLVQMLGPATLP
                     EPAGAARFEVLGPDDRPAMAALVRATEPGPWAERTPELGRFVGMKADGRLVAMAGERL
                     HLDGWHEISGVCTDPAWRGQGLARDAMLVVGRAMLARGERPFLHVFASNAPAIALYEK
                     LGFVQRARRWATGLQRLG"
     gene            complement(749..1060)
                     /locus_tag="VF453_10965"
     CDS             complement(749..1060)
                     /locus_tag="VF453_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688222.1"
                     /translation="MNTSTTPRSSAPLREADGRDGRVVGDNDDSPMESLGKAVVAPIE
                     GADEGSQRPGSSGDRRAEPVDGEHGRAFVDLTRTLPQGEDETAKAEGAAPVPKPDGKA
                     L"
     gene            complement(1299..2273)
                     /locus_tag="VF453_10970"
     CDS             complement(1299..2273)
                     /locus_tag="VF453_10970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7688223.1"
                     /translation="MTNATRPPLDPQSHTWLELGEVQRLLLDGYPGLALGLDAEGRLQ
                     WLNPAVTERLGWQRDELVGQPLAGTLVAQPELEARAAQLKASSGRHVPADAGAFFASL
                     PAVAGPTEAQEWVLRHKDGSPQPARLAFGPLRDHSGAIVGLIAVEALAHPAEDAPLRL
                     THHDSLTGLPTRAVLQDRAEMALLRAARQKTVLALLLVDLGGFDAICEEYGHSVGDDV
                     LRATAGRLHFELRKTDTAVRLERAQFAAMLVDLHEPSQARLVAEKIVKSLSAPINVGV
                     ARLAVAPRIGVVWTPTHGDQLLPMLQLAEAALATVPVDQAGIASGPET"
     gene            2501..>2716
                     /locus_tag="VF453_10975"
     CDS             2501..>2716
                     /locus_tag="VF453_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688224.1"
                     /translation="MKSTTVLVAAIAVLSAAFSVGARAESGRPSTLEAPAAPIAVVDD
                     AAAGSYARYLMLNGVDRDRALQMAASTD"
BASE COUNT          324 a         1020 c         1006 g          366 t
ORIGIN      
        1 atgcctttcc cgtccgaacc cgccgccgac catccgctcg acaacgtgat gtggtcggcg
       61 ctgaccaccc gccaggcgcc gctggcgcgc gggcagggtc tcgcgcgccg cttcgatccc
      121 gcctatgcgc gcttcgcggg ggtcgccgag ttcagccccg cggcgctcga cgcgctggcg
      181 gacctgctgg agcccggcga ggacgtgtcc gtcttcccgc ccgacgacgc gctcgacccg
      241 gggccgcgct tcgaggtgct gtaccgcaag ccgttggtgc agatgctggg gcccgcgacg
      301 ctgcccgagc ctgccggcgc cgcgcgcttc gaggtgctgg ggccggacga ccgccccgcg
      361 atggcggcgc tcgtgcgcgc caccgagcca ggcccgtggg ccgagcgcac gcccgagctc
      421 gggcgcttcg tcggcatgaa ggcggacggg cggctagtcg cgatggcggg cgagcgcctg
      481 cacctggacg gctggcacga gatcagcggc gtctgtaccg atcccgcgtg gcgcggccag
      541 ggcctcgcgc gcgatgcgat gctggtcgtg ggccgggcga tgctcgcgcg cggcgagcgg
      601 cccttcctgc acgtgttcgc gagcaacgcg cccgcgatcg ccctgtacga gaagctcggc
      661 ttcgtgcagc gcgcgcggcg ctgggcgacc ggcctgcagc gcctgggatg acgacgcccg
      721 tgcgaccctg agcgagcggg ccggatcgtc agagcgcctt gccgtccggc ttgggcaccg
      781 gcgccgcgcc ctcggccttg gcggtctcgt cctcgccctg cggcagcgtg cgcgtgaggt
      841 cgacgaaggc gcggccgtgc tcgccgtcga ccggctcggc gcggcgatcg ccgctgctgc
      901 cgggccgttg ggagccctcg tccgcgccct cgatgggggc gacgacggcc ttgccgagcg
      961 attccatcgg gctgtcgtcg ttgtcgccga cgacgcggcc gtcgcggccg tcggcttcgc
     1021 gcaggggcgc ggaagagcgg ggggtggtcg aggtgttcat cgcgatctcc ggattcggtg
     1081 ggactgcgcc cggatcggca agccccattc ctggagcgcg cccgcgtcgc tcccgacgag
     1141 ccagcgcgag gacgacaccc cccggcgctg ccgctcacac gcgtccccga gacacgggaa
     1201 agcgacggat ccggctccgc cggtccgtgc gcgcacgccc cctcgggggg cagcgagcgc
     1261 ccgcgagcgt ggggactcag gccctcgagc gtgggggctc aggtctccgg cccgctcgcg
     1321 atgccagcct ggtcgacggg cacggtcgcg agcgcggcct cggcgagctg cagcatcggc
     1381 agcagctggt cgccgtgcgt gggcgtccag accacgccga tgcgcggcgc gaccgcgagc
     1441 cgcgcgacgc ccacgttgat cggcgcggac agcgacttca cgatcttctc ggccaccagc
     1501 ctggcctggc tcggctcgtg caggtcgacc agcatcgcgg cgaactgggc gcgctccagg
     1561 cgcaccgcgg tgtcggtctt gcgcagctcg aaatgcagcc ggccggcggt ggcgcgcagc
     1621 acgtcgtcgc cgaccgaatg gccgtactcc tcgcagatcg cgtcgaagcc gccgaggtcg
     1681 accagcagca gcgcgagcac cgtcttctgg cgcgcggcgc gcagcagcgc catctcggcg
     1741 cggtcctgca gcacggcgcg cgtgggcagg ccggtcaggc tgtcgtggtg ggtgaggcgc
     1801 agcggggcgt cctcggcggg gtgggcgagc gcctcgaccg cgatcagccc cacgatggca
     1861 ccggagtggt cgcgcaacgg cccgaaggcg agccgcgccg gctgcggcga gccgtccttg
     1921 tggcgcagca cccactcctg cgcctcggtc gggcccgcga ccgccggcag cgacgcgaag
     1981 aaggcgcccg cgtcggccgg cacgtgcctg ccgctggagg ccttcagctg cgcggcgcgc
     2041 gcttccagct cgggctgggc caccagcgtg ccggccagcg gctgcccgac gagctcgtcg
     2101 cgctgccagc ccagccgctc ggtgaccgcc ggattgagcc actgcaatcg cccctccgcg
     2161 tccaggccca gcgcgaggcc ggggtagccg tcgagcagca ggcgttgcac ttccccgagt
     2221 tccagccagg tgtgggactg cgggtccagg ggcgggcggg tggcgttcgt catcgggcca
     2281 gtctcgcacc gcccatcggc ggccgtcgat acccgcgccg ccggaagggg tgcggcgtcc
     2341 gactgttccc gccgcgattt caaggccgcg attgttcgca tcggcgaaag agtgcattgc
     2401 gcgccgtcgc gttgatgcgg atcagtggaa accctagatt cacgtcacgg ccccgagctc
     2461 cccggggtcc ttcgacaaac caccacgaaa gcgcatcgcc atgaagtcca ccaccgtcct
     2521 cgtcgccgcc atcgccgtcc tgtccgccgc cttctcggtc ggcgcccgtg ccgaaagcgg
     2581 ccgcccgtcc acgctcgagg cgccggccgc gccgatcgcc gtcgtcgacg acgccgccgc
     2641 cggctcgtac gcccgctacc tgatgctcaa cggcgtggac cgcgaccgtg cgctgcagat
     2701 ggccgcgtcg accgac
//
LOCUS       DASVLN010000454         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_398587, whole genome shotgun sequence.
ACCESSION   DASVLN010000454 DASVLN010000000
VERSION     DASVLN010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398587"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..814
                     /gene="cyoC"
                     /locus_tag="VF453_10980"
     CDS             176..814
                     /gene="cyoC"
                     /locus_tag="VF453_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HEX7688225.1"
                     /translation="MSSVTYQQSQTFGAHPHADGVDHEHSHDAGGNTVFGFWIYLMSD
                     CLLFACLFAGFAVLGRNTAGGPSAKELFDLKYVAVETGALLLSSITYGMAMIGSLKRN
                     STKVLLWLAITALFGLVFIGMEVNEFHHLIEEGNGPNRSAFLSAFFSLVGTHGIHVTS
                     GLIWLAVLMYQVLTKGVTDTNRTRLMCLSLFWHFLDIVWICVFTVVYLMGVL"
     gene            816..1265
                     /gene="cyoD"
                     /locus_tag="VF453_10985"
     CDS             816..1265
                     /gene="cyoD"
                     /locus_tag="VF453_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEX7688226.1"
                     /translation="MASNFPQSQIDPASGTFGRRNAARYVGGTVAGESHASAKSYLIG
                     FVLSVILTAIPFGLVMDETHHGFSTDTVLAAILVFAVVQVFVHVVYFLHMDRSAEQRW
                     NVMAFAFTILILAIVVAGSVWIMHNATTNMAHGPMQPTGQEAVIPQR"
BASE COUNT          220 a          540 c          483 g          270 t
ORIGIN      
        1 ctgggcttcg gcctgatctg gcacatctgg tggctggccg tcgtcggctt cgtcggcatg
       61 atcgccgcct tcgtcgtgcg ctcgttcgac gacgacgtgg actactacgt gccggccgcc
      121 gaggtcgcgc gcatcgagaa cgcaagccgc ctcgcggcca accaggggta agccggtgtc
      181 gagcgtcacc tatcaacaat cgcagacctt cggcgcgcat ccgcacgccg atggcgtcga
      241 ccacgagcac tcgcacgacg cgggaggcaa caccgtcttc gggttctgga tctacctgat
      301 gagcgactgc ctgctgttcg catgcctgtt cgccgggttc gccgtcctgg gccgcaacac
      361 cgcgggtggc ccgtcggcca aggagctgtt cgacctcaag tacgtcgccg tcgagacggg
      421 cgccctgctg ctcagctcga tcacctacgg catggccatg atcgggtcgc tcaagcgcaa
      481 ctcgaccaag gtgctgctgt ggctggcgat cacggcgctg ttcggcctcg tcttcatcgg
      541 gatggaagtc aacgagttcc accacctgat cgaggaaggc aatggcccga accgcagcgc
      601 gttcctgtcg gcgttcttct cgctggtcgg cacgcacggg atccacgtca cctccggcct
      661 gatctggctg gccgtgctga tgtaccaggt gctgaccaag ggcgtcaccg acaccaaccg
      721 cacgcgcctg atgtgcctca gcctgttctg gcacttcctg gacatcgtct ggatctgcgt
      781 gttcaccgtg gtctacctga tgggcgtcct ctgacatggc ttcgaacttc cctcaatcgc
      841 agatcgaccc ggcatcgggt accttcggcc gccgcaacgc ggcgcgctac gtcggcggca
      901 cggtcgcggg cgaatcgcac gccagcgcca agtcctacct gatcggcttc gtgctgtcgg
      961 tgatcctgac ggcgatcccg ttcggcctcg tgatggacga gacgcaccac gggttctcga
     1021 ccgacacggt gctggccgcg atcctcgtgt tcgcggtggt ccaggtcttc gtgcacgtcg
     1081 tgtacttcct gcacatggat cgctcggccg agcagcgctg gaacgtgatg gcgttcgcct
     1141 tcacgatcct gatcctcgcg atcgtcgtcg ccggctcggt ctggatcatg cacaacgcga
     1201 cgaccaacat ggcgcacggc ccgatgcagc cgacgggcca ggaggccgtg atcccgcagc
     1261 gctgagctct gctctcgcac gacaagggcg ccccgcgggg cgcccttttc attggtgcgc
     1321 gcggcgcgcg cgcgcgagca ggccggcgcg cgggcgtcac gcgcctgccc ggggacggga
     1381 acggccgttg ccaagggcga caaccgaccc gcccgtcgcg acggcctcgc gcctcgccgc
     1441 ggcggcaacc cgtcccgcga agcgagatcg cccatgcccc agcctccgtc ccgccgctcc
     1501 tcgctgcgcc gcc
//
LOCUS       DASVLN010000455         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_77637, whole genome shotgun sequence.
ACCESSION   DASVLN010000455 DASVLN010000000
VERSION     DASVLN010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77637"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VF453_10990"
     CDS             <1..1204
                     /locus_tag="VF453_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX7688227.1"
                     /translation="VLRPPSWPLAVKMVAALVLAAVLPMTITAWYNYTSNVDALASSE
                     RRNLERLALATSGRLSQLVEDSRRTTAYLATGDDIADLLAHPTDEAKRAVLARLSNIV
                     ATNPDLHLLTLMDARGTALVSTAPEVVGGNYAFREYFKTAMSGQSFITGIIVGSVAGR
                     AGVYVAYPVRRDGRVIGVIALRLRAEALDAIVDGARSLNREPFLVDGDGVLIHHRIDS
                     LRFHSLAELSAREQAEIAADQRFRLKHVDGLGMPGLAHAMVGAKHEGTIDYRSTISRT
                     REVAGYAPVAGVDWVVGVTESRDSFEAPLRAIFEQVLYSLAVVGAIFVLLAILFARTI
                     SRPVQALTHAADALKRGDYDGARVRIKSGDEIGALGRTFNVMIDVLRQRERERGLRAG
                     PGEGRGKH"
     gene            1500..2714
                     /locus_tag="VF453_10995"
     CDS             1500..2714
                     /locus_tag="VF453_10995"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX7688228.1"
                     /translation="MSNNVFVGLDWAARSHAVCVVDDGGRILDRFEVAHDRDGLAALE
                     RRLARFGARLRVAIERPSGLVVDALVEAGHDVVPIHPNALKACRPRYRCHGGKSDAGD
                     AYILADVLRTDGHRWRALTPQSDGIQALRALVRARDDLVATRVALSNQLRSVLEAFWP
                     GAACVFADIASPIGLAFLRRYPSAASAARLNERQLRTFCRRQHYSGRRTAGELLQRLR
                     SAAPTFAGSAAIEASSRIVSSLSAVLKSVVEQIAELTRRIEQSIEALPDGRIIMSFPR
                     AGHVCAAQLLSELGDVRERFPSAEQLAAEAGTTPVTYASGKSHGVAFRWACNHRLRRA
                     VTCFADNSRHANAWAHSVYAAARARGCSHPHAVRILARAWIRVLWRAWMDRRPYDGEL
                     HLAAARLAAAGG"
     gene            complement(2726..3622)
                     /locus_tag="VF453_11000"
     CDS             complement(2726..3622)
                     /locus_tag="VF453_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688229.1"
                     /translation="MTNHADLPLHALACLDAVVTEGGFQAAAAKLNRTHSAVFAAIRQ
                     LEARAGVALLDRSGYRVALTDDGRAFHARACAVLAESAALGELAAQLRRGEETDLRVV
                     VGDLTPIAPVLRALKRFFDGCPRTRLHLHFEALGGPWERLFAGEADLIVHHADPADPR
                     LEREKLLAVTLVPVAAPGFLPFPLTRNLSPEAMRGLVQCILRDSAAQPGPSYHVVPGA
                     RSWTVADQQTKKELILRGMGWGHMPLHLVDRELRSGKLLSLEGRHFKRTRLDIVAARL
                     RGRPVGPVAARLWDMLASSVRA"
     gene            3721..>4200
                     /locus_tag="VF453_11005"
     CDS             3721..>4200
                     /locus_tag="VF453_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX7688230.1"
                     /translation="MRAIVRQAFGGPEQLEIREVPTPRPAAGQVLVRVRAFGLNRAEQ
                     YFRTGAWGDVAAISGIECVGEVAEDPAGRLGRGRRVLALMGGMGRSIAGSYAEYACVP
                     ASSVVPVHTTLGWAELAALPETWATAWSCLHDNLRIAPGERLVVRGANSALGQAALAL
                     "
BASE COUNT          588 a         1547 c         1464 g          601 t
ORIGIN      
        1 cgtgctgcgg ccgccgtcct ggccgctggc ggtgaagatg gtcgcggcgc tggtgctcgc
       61 cgccgtgctg cccatgacca tcaccgcctg gtacaactac acgagcaacg tcgacgcgct
      121 cgcgagttcc gagcgccgca acctcgagcg gctcgcgctc gcgacctcgg gccggctgtc
      181 gcaactggtc gaggacagcc gccgcacgac cgcctatctc gccacgggcg acgacatcgc
      241 cgacctgctg gcgcatccca ccgacgaggc caagcgcgcg gtgctggcgc gcctgtccaa
      301 catcgtcgcg accaaccccg acctgcacct gctgacgctg atggacgcgc gcggcacggc
      361 gctggtctcc accgcgcccg aggtcgtcgg cggcaactac gccttccggg agtacttcaa
      421 gaccgcgatg tccgggcagt ccttcatcac cggcatcatc gtcggctcgg tggccggccg
      481 cgccggggtc tacgtcgcct accccgtgcg gcgcgacggc cgcgtgatcg gcgtcatcgc
      541 gctgcggctg cgcgccgagg cgctcgacgc catcgtcgac ggcgcgcgca gcctcaaccg
      601 cgagcccttc ctcgtcgacg gcgacggcgt gctgatccac caccgcatcg acagcctgcg
      661 cttccacagc ctggccgaac tctcggcgcg ggagcaggcc gagatcgccg ccgaccagcg
      721 cttccggctc aagcatgtcg acggcctggg catgcccggc ctcgcccatg cgatggtcgg
      781 cgcgaagcac gagggcacga tcgactaccg ctcgaccatc tcccggacgc gcgaggtcgc
      841 gggctacgcg ccggtcgcgg gtgtcgactg ggtggtcggc gtgaccgagt cgcgcgacag
      901 cttcgaggcg ccgctgcgcg ccatcttcga gcaggtgctg tacagcctgg cggtcgtcgg
      961 cgcgatcttc gtgctgctgg cgatcctgtt cgcgcgcacg atcagccggc cggtgcaggc
     1021 cctgacccac gcggccgacg cgctcaagcg cggcgactac gacggcgccc gggtgcgcat
     1081 caagagcggc gacgagatcg gcgccctcgg ccgcaccttc aacgtgatga tcgacgtgct
     1141 gcgccagcgc gaacgcgagc gcgggctgcg cgccgggccg ggcgagggac gcggcaagca
     1201 ctgatggcgc ggccgggctc ataaggcaac ctacccccca ttgatccaac gacgctggcg
     1261 ggttgactaa gccgagtcgt gggtcgaagg tattgccgac ttccgtgccc ctgcggagac
     1321 tgctgcggac gctcgcccct cgagggccag gctgagaccc gcggcctgca gggtgccgga
     1381 agaccccgac gggccacagg atgtagcgcc aggtttaccg agctggagcg ccaatttatg
     1441 agcttcccgc cgggtcttcc gacacggaca gtcataccgc aactgccggg agcttgtcca
     1501 tgagcaacaa cgtcttcgtc ggactggact gggcggcgcg ctcgcacgcg gtgtgcgtcg
     1561 tcgatgatgg cggtcgcatc ctcgatcgct tcgaggtggc gcatgaccgc gacggcttgg
     1621 ctgcgctcga gcgccggctg gcgcgcttcg gggcgcggct gcgcgtggcc atcgagcggc
     1681 cttccggcct cgtcgtcgat gcactcgtcg aggccgggca cgacgtggtg ccgatccacc
     1741 ccaacgccct caaggcttgc cgaccgcgct accgctgcca cggcggcaag agcgacgcgg
     1801 gcgacgccta catcctggct gacgtgctgc gcaccgacgg ccaccgctgg cgcgcgctga
     1861 ccccgcagtc cgacggcatc caggctttgc gcgcgctcgt tcgtgcccgc gacgacctcg
     1921 tggcgacgcg cgtggcactg tccaaccagc tgcgcagcgt cctcgaagcg ttctggcccg
     1981 gcgcggcctg cgtctttgcc gacatcgcct cgcccatcgg cctggccttc ctccggcgct
     2041 acccgtcagc tgcgtcggcg gcgcggctga acgagcgaca actgcgaacg ttctgcaggc
     2101 gacagcacta cagcgggcgc cgtacagcag gcgagctgct ccagcgcttg cgcagcgcag
     2161 cgccgacgtt cgcgggctcg gccgccatcg aggcatcgtc gcggatcgtc agttcgttga
     2221 gcgccgtgct caaatccgtg gtcgagcaga tcgccgagct cacgcgccgc atcgagcaat
     2281 ccatcgaagc gctgcccgac ggtcgcatca tcatgtcgtt tccgcgcgcg gggcacgtct
     2341 gtgccgccca actgctgtcg gagctggggg acgtgcgcga gcgcttcccg agtgccgagc
     2401 aacttgccgc cgaggcgggt accacacccg tcacctacgc gtcgggcaag tcacacggcg
     2461 tcgcattccg ttgggcctgc aaccaccgac tgcgtcgggc cgtcacttgc ttcgcagaca
     2521 actcgcgtca cgccaacgcc tgggcccact ccgtctacgc cgcggctcgg gcacgcggct
     2581 gcagccaccc gcacgccgtt cgcatcctgg ctcgtgcctg gatacgcgtg ctctggcgcg
     2641 cctggatgga ccgtcgtccc tacgacggcg aactccacct ggctgcagct cggctggccg
     2701 cagctggggg ttgacacagg ttgtctcagg cccgcaccga gctcgccagc atgtcccaca
     2761 gccgggcggc caccggcccc accggccgtc cgcgcaggcg cgcggcgacg atgtccaggc
     2821 gcgtgcgctt gaagtgccgg ccctccagcg acagcagctt gccgctgcgc agctcccggt
     2881 cgaccaggtg cagcggcatg tggccccagc ccatgccgcg caggatcagc tccttcttcg
     2941 tctgctggtc cgcgaccgtc cagctgcgcg cgcccggcac cacgtggtag ctcggccccg
     3001 gctgcgccgc ggagtcgcgc aggatgcact gcaccagccc cctcatcgcc tcgggcgaga
     3061 ggttgcgcgt gagcgggaag ggcaggaagc cgggcgccgc gacggggacg agcgtcaccg
     3121 ccagcagctt ctcgcgctcg aggcgcgggt cggcgggatc ggcatggtgc acgatgaggt
     3181 cggcctcgcc cgcgaacagg cgctcccacg gcccgccgag ggcctcgaaa tgcaggtgca
     3241 gccgcgtccg cgggcagccg tcgaagaagc gcttgagcgc gcgcagcacg ggcgcgatgg
     3301 gcgtgaggtc gccgacgacc acgcgcaggt cggtctcctc gccccggcgc agctgggcgg
     3361 cgagctcgcc cagcgccgcc gactcggcga gcacggcgca ggcgcgtgcg tggaaggcgc
     3421 ggccgtcgtc ggtgagcgcg acgcggtagc ccgagcgatc cagcagcgcc acgccggcgc
     3481 gcgcctcgag ctggcggatc gccgcgaaca ccgccgaatg cgtgcggttc agcttcgccg
     3541 cggccgcctg gaagccgccc tcggtcacca cggcatccag gcaggcgagc gcgtgcaggg
     3601 gaaggtcggc gtgattggtc atggaatcga caatccttgg cgaaatcttt cgatttcatc
     3661 gtttcggagg gctgcgtatt ctgcgccctc ccacaacgcg tcatcgaaag gaacgacccc
     3721 atgcgagcga tcgtgagaca ggccttcggc gggcccgagc aactcgagat ccgggaggtg
     3781 ccgaccccgc gccccgcggc cggccaggtt ctggtgcgcg tgcgcgcctt cggcctgaac
     3841 cgcgccgagc aatacttccg caccggcgcc tggggcgacg tggcggcgat ctcgggcatc
     3901 gagtgcgtcg gggaggtggc ggaggatccc gcgggccgcc tcggccgcgg ccggcgcgtg
     3961 ctcgcgctca tgggcggcat gggccgcagc atcgccggca gctacgccga gtacgcatgc
     4021 gtgccggcct ccagcgtggt gcccgtgcac acgaccctgg gctgggccga actggccgcc
     4081 ctgcccgaga cctgggccac cgcctggagc tgcctgcacg acaacctgcg gatcgcgccg
     4141 ggcgagcgcc tcgtcgtgcg aggcgccaac tcggcgctcg gccaggcggc gctcgcgctc
//
LOCUS       DASVLN010000456         7223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_77645, whole genome shotgun sequence.
ACCESSION   DASVLN010000456 DASVLN010000000
VERSION     DASVLN010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77645"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..2175)
                     /locus_tag="VF453_11010"
     CDS             complement(70..2175)
                     /locus_tag="VF453_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7688231.1"
                     /translation="MPSTFLPTAYDAWIVACSLLIASFASYVALDLAKRVRGPDPRVA
                     RRWWLGGSIALGSGIWCMHFVGMMALTLPIELGYGAVLTATSWVAAVATSAVALFAAS
                     RRTLRRAHVAGGALAMGAGICAMHYLGMAAIDLAPGIAWDWPLVAASVVIAVVASATA
                     LEIFVLLRHASPAHAPRYQLGAAIVMGAAICGMHYTGMAAANFPASTVCRTAGALSED
                     GVGAGVVAAAIALLVLALSTSYLDARMQARTAGLNDRLRTANEELRVRALRDPLTQLP
                     NRPHFEERLEQAFSDDGCGRLAVLFIDLDGFKPVNDALGHAVGDVVLQEVAARLRACV
                     RAADTVARIGGDEFVLLMACEDAQADPVAAARRIVEAIGTAIPTAARTVQVSCSVGIA
                     TWPEHGPRERLVAHADAAMYEAKRAGGNTWAHYEPRMEGAPAQMLGLQNDLRAAIDRR
                     ELELHYQPKVDGRTGRMHGVEALLRWKHPVHGLVGPVVFIPLAERHGLIGRLGNWVIE
                     EACRQIEAWAADGLRMNVAINLSVHQLREDDLVERIQQALRRHRVDAGQLLCEITESV
                     AMGDVKATQAAFSGLARIGVLLSIDDFGTGYSSLSYLRRLPARQLKIDRSFVSDLEAS
                     SDARAIVSAVVHLAHDLGLGVVAEGVETEGQRAVLAELDCDELQGYLFAKPMRADDVA
                     GWARAQDARVRAEAAAAAA"
     gene            2337..3641
                     /locus_tag="VF453_11015"
     CDS             2337..3641
                     /locus_tag="VF453_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7688232.1"
                     /translation="MSSPAAAHPRPAAAVRRPSAAATVLRVTGGNFLEMFDFFLYGFY
                     ATDIARAFFPVRDAYVSLLLTFVTFGAGFLMRPLGAIVLGAYIDRIGRRRGLILTLAL
                     MACGTVLIALVPAYATIGMLAPILVLAGRLLQGFSAGVELGGVSVYLSEMATPGRKGF
                     YVAWQSASQQAAIMVAALLGFVVDREFSRAQIDDWAWRIPFFIGCMILPFVFYVRRSL
                     VETDEFLARTLRPTLREIGASMARNAGLVVAGMMLVVMTTVSFYLITVYTPTFGKAVL
                     HLGAADALVVTFCVGASNFVLLPLMGALSDRVGRWPLLAGFTLLTILTAYPALAWLVA
                     APTFGRMLAVELWLSVLYASYNGAAVVALTEVMPAHVRTVGFSLAYALATAVFGGFTP
                     AVSTWLIAFTGDKAAPGLWMSGAAACGLVATWLIYRGFVKAR"
     gene            complement(3651..4064)
                     /locus_tag="VF453_11020"
     CDS             complement(3651..4064)
                     /locus_tag="VF453_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688233.1"
                     /translation="MNFLFDNNLSSHLAHGMRELSKNLEGVEEVIHLTDRFARNAPDL
                     EWITALKSPQAWYIVSVDRFRKQHGAEREAIRRAGHTVFVLDSQWSKHSFWLQAERLV
                     RWWPQIVTQARLVSGGGYRVPWAHSARIKFQAIPF"
     gene            complement(4061..4750)
                     /locus_tag="VF453_11025"
     CDS             complement(4061..4750)
                     /locus_tag="VF453_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX7688234.1"
                     /translation="MELIGIGLYPLAQAARLAGAEPRAVRRWLRGYNRRHRGQSVRSE
                     PLWETQLRDDALMEDVIGFRDLLELRMVATFVRHGVDLRVIRATLETARRDFGESHPL
                     TNRRFLSDGRRIFLEAIEQATGEARMIDVLRRQHVFSDIIRPSLYAGIEYADGGARRW
                     FPEGRRKVVVLDPALQFGTPVLTDAGIPTDTVHAAFLAEGRDRNSVARIFSITPAMVS
                     AAVEFEQRLAA"
     gene            complement(4848..6707)
                     /locus_tag="VF453_11030"
     CDS             complement(4848..6707)
                     /locus_tag="VF453_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7688235.1"
                     /translation="MPSKHPALTALALSVALAFAAVPAEAAKIAVKKEADLPRFSYAI
                     PGPASKFVEADDAAFGVFAAQVSRDIDKTLAEYQVDDHSMLGELLSARLDAQELSHDY
                     AGGLKTIEQLRALEDKPAAKLISGLYARARLQAAIDAGAESGPAYETAFKKHYAEAIA
                     PLPWAIVQDSIKSAYGSSQLASRALMLGHVQSELDPAAAKSGALDAGEARELLEARIY
                     LKEFLPISAARAEVLHAYIAKNQVSPPEIWSAREVTLTAADKTTPVNVGIWDSGVDVA
                     LFPDQLFTDDHPTASGTHGLAFDDEGNPSRDWLYPLTKAQIDGYPSFREEVQGRIDLQ
                     NGVDSKAARDTQQLFRTLSPEQMHERNEIGKVLGFYAHGTHCAGIAVRGNPAARLVVA
                     RFDDQLPDFPFQPTEAWVRKMGADFQQMADYFRTRHVRVVNMSWGDDVSEFETWLSKT
                     GGGGDPQARKAKAAALFALWKAAIEQAIKSAPDTLFVTAAGNSDSDAGFLEDVPASLH
                     LPNLIAVGAVNKAGDETSFTSHGDTVLAHADGFQVDSFVPGGARVPFSGTSMASPNVV
                     NLAAKLIALDPTLTPAQTIALIREGATTSDDGRRHLIDEKKSVALLRTRMAAR"
     gene            complement(6846..>7223)
                     /locus_tag="VF453_11035"
     CDS             complement(6846..>7223)
                     /locus_tag="VF453_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688236.1"
                     /translation="RADDLDASGAGARLYHRLDLATVPSSLAQARPADHYHLDDFKSA
                     QVSLDSSSVTMHDHGFFYEMRVLAVGDFRRVGHGEFLVRFVERSETGGSYDRTAILVV
                     DPAPGGGLVATDALDVLRGDAGR"
BASE COUNT          966 a         2525 c         2627 g         1105 t
ORIGIN      
        1 gcttcgccga cgtgcagcgc tacctgcgcg cgcagggcgc gccctacgcg ggcggctccg
       61 gccgttgact cacgccgcgg cggccgccgc ctcggcacgg acgcgggcat cctgcgcgcg
      121 tgcccagccg gcgacgtcgt ccgcgcgcat cggcttcgcg aacaggtagc cctggagctc
      181 gtcgcagtcc agctcggcga gcaccgcgcg ctggccttcg gtctcgacgc cttcggccac
      241 gacgcccagc ccgaggtcgt gcgcgaggtg cacgacggcg ctgacgatgg cgcgggcgtc
      301 gctgctcgcc tcgaggtcgc tcacgaagct gcggtcgatc ttgagctggc gcgcgggcag
      361 ccggcgcagg tagctcaggc tcgagtagcc ggtgccgaag tcgtcgatcg acagcagcac
      421 cccgatgcgc gcgaggccgc tgaacgccgc ctgcgtggcc ttgacgtcgc ccatcgcgac
      481 ggactcggtg atctcgcaca gcaactggcc ggcgtcgacg cggtgacggc gcagtgcctg
      541 ctggatgcgc tcgacgagat cgtcctcgcg cagctggtgc accgagaggt tgatcgcgac
      601 gttcatgcgc agcccgtccg cggcccaggc ctcgatctgg cggcaggctt cctcgatgac
      661 ccagttgccc agcctgccga tcaggccatg gcgctcggcc aaggggatga agacgacggg
      721 gccgaccagg ccgtgcacgg gatgcttcca gcgcagcagc gcctcgacgc cgtgcatgcg
      781 gccggtgcgg ccgtcgacct tgggctggta gtgcagctcg agctcgcgcc ggtcgatcgc
      841 cgcgcgcagg tcgttctgca gtcccagcat ctgcgcgggc gcgccttcca tgcgcggctc
      901 gtagtgcgcc caggtgttgc cgccggcacg cttggcctcg tacatcgccg cgtcggcgtg
      961 cgcgaccagg cgttcccggg ggccgtgctc aggccaggtc gcgatgccca ccgagcacga
     1021 gacctgcacc gtccgcgcgg cggtcgggat cgccgtgccg atcgcctcga cgatgcgccg
     1081 tgccgcggcc accgggtcgg cctgcgcgtc ctcgcaggcc atcagcagca cgaactcgtc
     1141 gccgccgatg cgggcgaccg tgtcggccgc gcgcacgcag gcgcgcaggc gggccgcgac
     1201 ttcctgcagc acgacgtcgc cgaccgcgtg gccgagcgcg tcgttgaccg gcttgaagcc
     1261 gtcgaggtcg atgaacagca ccgcgagccg gccgcagccg tcgtcggaga acgcctgctc
     1321 gaggcgctcc tcgaagtggg gccggttcgg cagctgcgtg agcggatcgc gcagcgcgcg
     1381 cacgcgcagc tcctcgttgg ccgtgcgcaa ccggtcgttc aggcccgcgg tgcgcgcctg
     1441 catgcgggca tccaggtacg aggtcgacaa cgcgagcacg agcagcgcga tcgccgccgc
     1501 cacgacgccg gcgcccacgc cgtcctcgga cagcgcgccc gccgtgcggc acaccgtgct
     1561 cgcggggaag ttcgcggccg ccatgccggt gtagtgcatg ccgcagatcg ccgcgcccat
     1621 cacgatcgcc gcgccgagct ggtagcgcgg cgcatgcgcg gggcttgcgt ggcgcagcag
     1681 cacgaagatt tcgagcgccg tcgccgaggc gacgaccgcg atcacgaccg aggccgcgac
     1741 cagcggccag tcccaggcga tgcctggcgc gaggtcgatc gccgccatgc cgaggtagtg
     1801 catcgcgcag atgcccgcgc ccatcgcgag cgcgccgccg gcgacgtgcg cgcggcgcag
     1861 cgtccggcgg ctggcggcga acagggcgac cgccgaggtc gcgacggccg ccacccacga
     1921 ggtggccgtc agcacggcgc cgtagcccag ctcgatcggc agcgtcagcg ccatcatgcc
     1981 gacgaagtgc atgcaccaga tgccgctgcc gagcgcgatc gagccgccca gccaccagcg
     2041 ccgcgcgacg cgcgggtcgg ggccgcgcac gcgcttggcc aggtcgaggg cgacatagga
     2101 cgcgaagctc gcgatcagaa gcgagcaggc gacgatccag gcgtcgtagg cagtgggcag
     2161 gaaggtcgag ggcatggctg gatccggcag gcccgcggcg cggctgcgcg gacccccgag
     2221 caacgacttc gaccgcctgc gcgggaacct gagccgtgtc gccgtgaagg acgccacgcg
     2281 gggccgccgt ttgcccgttg ccgcctccac cgcccggcat cgccaggagc ccgcgcatga
     2341 gttcgcccgc cgccgcccat ccccgccccg ccgccgcggt gcgccgcccg tccgccgccg
     2401 cgaccgtgct gcgcgtgacg ggcggcaact tcctggagat gttcgacttc ttcctgtacg
     2461 gcttctacgc gaccgacatc gcccgcgcgt tcttccccgt gcgcgacgcc tacgtctcgc
     2521 tgctgctgac cttcgtcacg ttcggcgccg gcttcctcat gcggccgctc ggggcgatcg
     2581 tcctgggcgc ctacatcgac cgcatcggcc gccggcgcgg gctcatcctg acgctcgcgc
     2641 tgatggcctg cggcaccgtg ctgatcgccc tcgtgcccgc gtacgcgacg atcggcatgc
     2701 tcgcgccgat cctcgtgctc gcggggcggc tgctgcaggg cttctccgcg ggcgtcgagc
     2761 tgggcggtgt gtcggtgtac ctgtcggaga tggcgacgcc cggccgcaag ggcttctacg
     2821 tcgcgtggca gtcggccagc cagcaggccg cgatcatggt ggcggcgctg ctgggcttcg
     2881 tcgtcgaccg cgagttctcg cgcgcgcaga tcgacgactg ggcctggcgc atcccgttct
     2941 tcatcggctg catgatcctg ccgttcgtgt tctacgtgcg ccgctcgctg gtcgagaccg
     3001 acgagttcct cgcgcgcacg ctgcgcccga cgctgcgcga gatcggcgcc tcgatggcgc
     3061 gcaacgcggg gctggtcgtc gccgggatga tgctggtcgt gatgacgacc gtgtcgttct
     3121 acctcatcac cgtctacacg ccgaccttcg gcaaggccgt gctgcacctg ggcgcggcgg
     3181 acgcgctggt cgtcaccttc tgcgtcggcg cgtccaactt cgtgctgctg ccgctgatgg
     3241 gcgcgctgtc cgaccgcgtc ggccgctggc cgctgctggc cggcttcacg ctgctgacga
     3301 tcctcaccgc ctatccggcg ctcgcctggc tcgtggcggc gcccacgttc gggcgcatgc
     3361 tggcggtgga gctgtggctg tcggtgctgt acgcgagcta caacggcgcc gccgtggtcg
     3421 cgctcacgga ggtgatgccg gcgcacgtgc gcacggtcgg cttctcgctc gcctacgcgc
     3481 tggcgaccgc cgtcttcggc ggcttcacgc ccgccgtctc gacctggctg atcgcgttca
     3541 ccggcgacaa ggcggcgccg ggcctctgga tgagcggcgc cgccgcctgc ggcctcgtcg
     3601 ccacctggct gatctaccgc gggttcgtca aggcgcgctg agcggcccgc tcagaacgga
     3661 atggcctgga acttgatcct tgcggagtgg gcccagggaa cccggtagcc gcctccggag
     3721 acgagacggg cttgcgtgac gatctgcggc caccatcgca cgaggcgctc ggcctgcagc
     3781 cagaagctgt gcttcgacca ttgggaatct aggacgaaga ccgtatggcc ggcgcgtcgg
     3841 atggcctcgc gctccgcacc gtgctgcttc cggaatcgat cgacggagac gatgtaccac
     3901 gcctgaggcg acttcaaggc cgtgatccac tccaggtcgg gagcgttgcg cgcgaagcgg
     3961 tcggtcaagt ggatgacctc ttcaacccct tccagattct tcgacagttc ccgcatcccg
     4021 tgggcaagat gcgaggaaag gttgttgtcg aagaggaagt tcaagccgcc agcctttgct
     4081 cgaattcgac ggctgccgag accatcgccg gcgtgatgct gaatatcctg gcgacggaat
     4141 ttcggtcgcg accttcggcg aggaaggcgg catgcacggt atcggtcggg atgccggcgt
     4201 ccgtcagcac gggtgtgccg aactgaaggg cgggatcgag cacgacgacc ttgcgccggc
     4261 cttctgggaa ccagcggcgt gcgccgccgt cggcatactc gatgccggcg tagagggacg
     4321 gcctgatgat gtccgagaag acgtgctgcc tgcgcaggac gtcgatcatg cgcgcttcgc
     4381 cggtcgcctg ttcgatggcc tccagaaaga tccgcctgcc gtcggagagg aaccggcgat
     4441 tggtcagtgg atggctttcg ccgaagtccc ggcgggccgt ctccagcgtt gcccggatga
     4501 cgcgcaagtc gacgccatgg cggacgaagg tcgccaccat gcgcagctcg agcagatccc
     4561 ggaagccgat gacgtcctcc ataagggcgt cgtccctgag ctgcgtttcc cacagcggct
     4621 cggagcggac cgactggccg cgatgccttc ggttgtagcc gcgcagccat cgacggaccg
     4681 ctcgcggctc cgccccggcc agccgggcgg cttgcgcgag tgggtagagg ccgatcccga
     4741 tcagttccat gtgtcgacgc gaaagccgcc agtatgccaa ccggcggctg gccctgccat
     4801 ggacaaatcg gctgatttcc gccgaagacg ccgccgcccg cggcgcctca gcgcgccgcc
     4861 atccgcgtgc gcagcagcgc gaccgacttc ttctcgtcga tcaggtggcg gcggccgtcg
     4921 tcgctggtgg tcgcgccctc gcggatcagg gcgatggtct gcgccggcgt cagggtcggg
     4981 tcgagcgcga tcagcttggc cgcgaggttg acgacgttgg gcgaggccat cgaggtgccc
     5041 gagaacggca cgcgcgcgcc gcccggcacg aagctgtcga cctggaagcc gtcggcatgc
     5101 gcgagcaccg tgtcgccgtg gctcgtgaac gaggtctcgt cgcccgcctt gttgaccgcg
     5161 ccgacggcga tcaggttggg caggtgcagc gaggcgggca cgtcctcgag gaagccggcg
     5221 tcgctgtcgc tgttgccggc ggccgtcacg aacagcgtgt cgggtgcgga cttgatcgcc
     5281 tgctctatcg ccgccttcca cagcgcgaac agcgccgcgg ccttggcctt gcgcgcctgc
     5341 gggtcgccgc cgccgccggt cttggacagc caggtctcga actcggagac gtcgtcgccc
     5401 cagctcatgt tgaccacgcg cacgtggcgg gtgcggaagt agtcggccat ctgctggaag
     5461 tcggcgccca tcttgcgcac ccaggcctcg gtgggctgga acgggaagtc ggggagctgg
     5521 tcgtcgaagc gcgcgacgac caggcgcgcc gccgggttgc cgcgcacggc gatgcccgcg
     5581 cagtgcgtgc cgtgggcgta gaagccgagc accttgccga tctcgttgcg ctcgtgcatc
     5641 tgctcgggcg acagcgtgcg gaacagctgc tgcgtgtcgc gcgcggcctt ggagtcgacg
     5701 ccgttctgca agtcgatgcg gccctgcacc tcctcgcgga acgacgggta gccgtcgatc
     5761 tgcgccttgg tgagcggata gagccagtcc ctggacgggt tgccctcgtc gtcgaacgcg
     5821 aggccgtgcg tgccgctggc ggtggggtgg tcgtcggtga acagctggtc ggggaacagc
     5881 gcgacgtcga cgcccgagtc ccagatgccg acgttgaccg gcgtcgtctt gtcggcggcg
     5941 gtcagcgtga cctcgcgcgc cgaccagatc tcgggcggcg agacctggtt cttcgcgatg
     6001 taggcgtgca gcacctcggc gcgcgcggcg ctgatcggca ggaattcctt caggtagatg
     6061 cgcgcctcga gcagctcgcg cgcctcgccg gcgtcgagcg cgccggactt cgcggcggcg
     6121 ggatcgagct ccgactgcac gtgcccgagc atcagcgcgc ggctggcgag ctgcgaggag
     6181 ccatacgcgc tcttgatgct gtcctgcacg atcgcccacg gcagcggcgc gatggcctcg
     6241 gcgtagtgct tcttgaacgc cgtctcgtag gcggggccgg actcggcgcc ggcgtcgatc
     6301 gcggcctgca ggcgcgcgcg cgcgtacagg ccggagatca gcttggctgc gggcttgtcc
     6361 tcgagcgcgc gcagctgctc gatcgtcttc aggccgcccg cgtagtcgtg cgagagctcc
     6421 tgcgcgtcca ggcgcgcgct gagcagctcg ccgagcatcg agtggtcgtc gacctggtac
     6481 tccgcgaggg tcttgtcgat gtcgcggctc acctgcgcgg cgaagacgcc gaacgcggcg
     6541 tcgtcggcct cgacgaactt cgaggccggg cccgggatcg cgtagctgaa gcgcggcagg
     6601 tcggcttcct tcttgacggc gatcttcgcg gcctcggccg gcacggcggc gaaggcgagg
     6661 gccacgctca gggcgagcgc ggtgagggcg gggtgcttgg acggcatggg cgtcacctgg
     6721 cgttgttgtc gtgggcgcgt tatacccgcg cgcaggccgg cgtccgagcg ggctcggacg
     6781 gattgacgaa accgggggcc ggacgacgcg gccacccgcg tgggcgcgag ggaaatccct
     6841 cggcttcagc ggcctgcgtc gccgcgcagc acgtccaggg cgtcggtggc gacgaggccg
     6901 ccgcccggcg cgggatccac gacgaggatc gccgtgcggt cgtagctgcc gccggtctcg
     6961 gagcgctcga cgaagcgcac caggaactcg ccgtgcccca cgcggcggaa gtcgccgacg
     7021 gcgagcacgc gcatctcgta gaagaagccg tggtcgtgca tcgtgacgga cgacgaatcg
     7081 agcgacacct gcgccgactt gaagtcgtcg aggtggtagt gatcggcggg ccgcgcctgc
     7141 gcgagcgagc tcggcacggt cgccaggtcg agccggtggt agaggcgcgc accggcgccg
     7201 ctggcgtcga ggtcgtccgc ccg
//
LOCUS       DASVLN010000457         9819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_131826, whole genome shotgun sequence.
ACCESSION   DASVLN010000457 DASVLN010000000
VERSION     DASVLN010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131826"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="VF453_11040"
     CDS             complement(<1..759)
                     /locus_tag="VF453_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7688237.1"
                     /translation="MTSPTTPTVAAPPERGASAAITLLLAFACGLIAANLYYAQPLAG
                     PIGASLGLAPAATGLIVTLTQVGYGLGLLFIVPLGDLVESRRLVRTLIALAGLALLGA
                     ALSTRPAPFLAAVLAIGLVSVAAQVLVPYAAHLAPEAHRGRVVGNVMSGLLTGIMLAR
                     PAASFIAQAASWHAVFFLSAAAMAALAVLMGTALPPRRPSATLRYVELHASMARLART
                     TPVLQRRAAYQAAMFGAFSVFWTTTPLLLARDWHL"
     gene            complement(833..1462)
                     /locus_tag="VF453_11045"
     CDS             complement(833..1462)
                     /locus_tag="VF453_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7688238.1"
                     /translation="MPAAHAALAPHADSPGRGRPREFDVDEVLDKAIAVFCERGYHAT
                     SIGDLTAATGLASGSVYKAFGDKRGLYIAALDRYKAVRDAELRAALAPAPDGRARVRV
                     ALTHYAEASHGARGRLGCFVVGSAAELATFDDELSRRAAAALRRNETLLTGLLREGRA
                     DGSIPDHVDPEITGRLLLCLIQGMRVVGKTGTQRKDMLALVDIAMKTVA"
     gene            complement(1568..1852)
                     /locus_tag="VF453_11050"
     CDS             complement(1568..1852)
                     /locus_tag="VF453_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HEX7688239.1"
                     /translation="MDIASIHNNHEKAVFAAVQEMAEHFPHIKDNPDMLADIACVALN
                     RLPPRYIRHDVDFAFFQTDRERTESERSIAEAVEFAFGFVQARNAMRARS"
     gene            complement(1999..2868)
                     /gene="nadC"
                     /locus_tag="VF453_11055"
     CDS             complement(1999..2868)
                     /gene="nadC"
                     /locus_tag="VF453_11055"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX7688240.1"
                     /translation="MFDHDETLAEARARNVRDALFEDIGRADWTSLLIPAGRRVQASV
                     IVRESAVLCGRDWFCSTLSTLDPEARIDWMYGEGATMTADSEVCRIEADARALLSAER
                     PALNFLQLLSGTATVTRRHVEAIEGASTSPRGCAVLDTRKTLPGLRQAQKYAVRVGGG
                     RNQRLALWHGILIKENHIAAAGGIAQVMAQALALDAGVDIQIEVESVDQLREALDAGA
                     TSVLLDNFSLAAMREAVAVAKGRAVLEVSGGVTLSQLREIAATGVDRISVGKLTKDVA
                     ATDFSMRVTGEIA"
     gene            complement(2898..4040)
                     /gene="nadA"
                     /locus_tag="VF453_11060"
     CDS             complement(2898..4040)
                     /gene="nadA"
                     /locus_tag="VF453_11060"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX7688241.1"
                     /translation="MTTQATYEYDRQDAQGATCTASAWAKVLPVPGPDERARLKARAK
                     ELLKARDAALVAHYYVDGDLQDLALETGGCVADSLEMARFGRDAPQRTLVVAGVRFMG
                     ETSKILSPAKRVLMPDLDATCSLDLGCPAEDFAAFCDAHPDRTVVVYANTSAAVKARA
                     DWMVTSSCALAVVSHLKARGEKILWAPDRHLGRYIQEQTGADMLLWNGACIVHDEFKG
                     LELELLKRAHPDAMILVHPESPRSVVEMADVVGSTSQMLDAVVRGSAKEYIVATDNGI
                     LHRMRQLAPGKTLIEAPTAGNSATCKSCAHCPWMAMNGLQGLVDCLEHDTGAIEVAEP
                     VRAQAEGCIRRMLDFVAANPSAIAKAAKDATGRPAAAGIVPGIGAA"
     gene            complement(4154..4876)
                     /locus_tag="VF453_11065"
     CDS             complement(4154..4876)
                     /locus_tag="VF453_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688242.1"
                     /translation="MPRPSVLTLPNALRALREQLAPLVLPVKDVPPSGPSSMFDSAGA
                     LGVTVEVPESEQGALALHAHLASSPEVSAMLVVDPAGVWAHGMIQTLSDATGSPVDRL
                     RVHSRGGLQIRAVVDETLLPAGNGPHRLVRCLHGDARPADRRAVFGALLAHSQICSVL
                     VGPMPSADAVALVDEMHRLALQPFAANVRWLFYLGADHRGLESQIESRDWPVPPTVLH
                     ARPLNRSVTTVWNSLYEAWIGS"
     gene            5099..6853
                     /locus_tag="VF453_11070"
     CDS             5099..6853
                     /locus_tag="VF453_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX7688243.1"
                     /translation="MSPSPVPPALAPARPRLAGHALVEALVAQGVDTVFGVPGESFLA
                     ALDGFHEHAGRIRFVACRHEGGAAFMAEAHGKLSGRPGVCFVTRGPGATNASIGVHVA
                     FQDSTPMVLLVGQVAADQRDREAFQELDYRQMFGPGTLGLAKWVGEVQSADRLPEYVA
                     RAFRTAMQGRPGPVVLALPEDMLATLTAAPVLPRVEPARAWPEPQAVERLRQLLIRAE
                     RPIAIVGGSGWTADAAWELERFAEAWELPVACAFRFQDTFDNRHRLYAGDVGIGIAPA
                     LAQRIRDADLVLAIGVRLGEMTTGGYSLLQAPVARQPLVHIHAGAEELGRVYQPTLAL
                     QCAMAGVGGVLAELDPPAPRSPRAGAQAHADYLANLEAAAVEPMDLAAVVKTAGALAP
                     AGTVWTNGAGNYAGWLHRFHPYEGLQHRGRTQLAPTAGAMGYAVPSAVAASLLQPDRT
                     VVGISGDGDFLMTAQELATATAHGAGRGAGRLVVVVVDNGQYGTIRMHQEREFPARVS
                     GTALANPDFVALAHAFDWAAERVDDTAAFEPAWRRALAADRPTLLHLILPADRITSRT
                     TLAALREAALAHGAASGG"
     gene            complement(7505..7744)
                     /locus_tag="VF453_11075"
     CDS             complement(7505..7744)
                     /locus_tag="VF453_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688244.1"
                     /translation="MKHVFESIHRHAVTKAKIRFVATWTIADEALSWQAAVSLRSGVR
                     TLAGGGLALPDPERGGDPQAAVQRDVGKSIDALDV"
     gene            complement(7899..7975)
                     /locus_tag="VF453_11080"
     tRNA            complement(7899..7975)
                     /locus_tag="VF453_11080"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7939..7941),aa:Pro,seq:tgg)
     gene            complement(7988..9484)
                     /locus_tag="VF453_11085"
     CDS             complement(7988..9484)
                     /locus_tag="VF453_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HEX7688245.1"
                     /translation="MTRFVMCWGTSSGAGKSWLATALCRVAARRGIDVRPFKAQNMSN
                     NARVVAGLDGRGAEIGAAQWFQALAAGVEPTVDMNPVLLKPERDTASQVVVHGRVDAA
                     LSRQGWRERSAALAGFAAQSLARLAGGCELVVIEGAGSPAEINLAPQDYVNLATARQA
                     RGLGELASLLVADIDRGGAFAHLYGTWALLPADLRPTLRGFVLNRFRGDAALLEPGPA
                     QLRQLTGVALAGTIPMVRDHGLPDEDGVHDAGDPLAPKTGRRRVAVIAGPHLSNLDEF
                     EPLRRLPGVQLAWVRGLQDLGDADWIVLPGSKQVSGDLAWMRVQGLDAAVARHAAAGR
                     PVLGVCGGLQMLGEALVDPEGHDGEPGFAGPGLGLLPLVTRYEADKRVRRARLRFEPL
                     EGPWAPWAGCEADGYEIHCGVTVPHPDMAAPTVALRDAGGAPIGWQRGCVLGVYAHGL
                     LESPALLRALFGASVPALDATFDRLADLVERHLDARLLADILGRGPRT"
     gene            complement(9481..>9819)
                     /locus_tag="VF453_11090"
     CDS             complement(9481..>9819)
                     /locus_tag="VF453_11090"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine nucleotide hydrolase"
                     /protein_id="HEX7688246.1"
                     /translation="ARFPLWGEPRAALARELLDRGVRARVVCVDTARLDAAFCGRAYD
                     AAFLADIPNTVCPVGEDGEFHTFVEDGPGFAAPLRLRSLGQRRVASRPPFAPTTFVFE
                     RLALEAGDAR"
BASE COUNT         1286 a         3538 c         3701 g         1294 t
ORIGIN      
        1 caggtgccag tcgcgcgcca gcagcagcgg cgtggtcgtc cagaacacgc tgaaggcgcc
       61 gaacatcgcc gcctggtagg ccgcgcggcg ctggagcacg ggcgtcgtgc gcgcgaggcg
      121 ggccatcgag gcgtgcagct cgacgtagcg cagcgtggcg ctcggccggc gcggcggcag
      181 cgccgtgccc atcagcaccg cgagcgccgc cattgccgcg gccgacagga agaacaccgc
      241 gtgccacgac gccgcctgcg cgatgaagct ggcggcgggc cgcgcgagca tgatgccggt
      301 gagcaggccg ctcatcacgt tgccaacgac gcggcctcgg tgcgcctcgg gcgcgaggtg
      361 cgcggcgtag ggcacgagca cctgcgccgc gaccgagacc agcccgatcg ccagcaccgc
      421 cgcgaggaag ggcgccggcc gcgtcgacag cgcggcgccg agcaaggcga gccccgccag
      481 cgcgatcagc gtgcgcacga gccggcgcga ttcgacgagg tcgcccagcg gcacgatgaa
      541 cagcaggccg aggccgtagc cgacctgcgt cagggtgacg atcaggccgg tcgccgccgg
      601 cgcgaggccc agcgacgcgc cgatcggccc ggccagcggc tgcgcgtagt agaggttggc
      661 cgcgatcagc ccgcaggcga aggccagcag cagcgtgatc gcggccgagg cgccgcgctc
      721 cggcggggcg gcgacggtcg gggtggtcgg tgaggtcatg gcgtggtggt cgcttttatt
      781 ggggaacggt catgtccaaa atggacgagc gggtgacgcg atgcggagga cttcaggcca
      841 cggtcttcat cgcgatgtcc acgagcgcca gcatgtcctt gcgctgggtg cccgtcttgc
      901 cgacgacacg catgccctgg atcaggcaca gcagcaggcg gccggtgatc tccgggtcga
      961 catggtcggg gatcgagccg tcggcgcggc cttcgcgcag caggccggtc agcagcgtct
     1021 cgttgcggcg cagggcggcg gcggcgcgcc gggagagctc gtcgtcgaag gtcgcgagtt
     1081 ccgccgcgct gccgacgacg aagcagccca ggcggccgcg tgcgccgtgc gaggcctcgg
     1141 cgtagtgggt cagcgcgacc cgcacgcgcg cacggccgtc aggcgcgggc gcgagcgccg
     1201 cgcgcagctc ggcgtcgcgg acggccttgt agcggtcgag ggcggcgatg tagaggccgc
     1261 gcttgtcgcc gaaggccttg tagacgctgc cggaggccag gccggtggcg gcggtcaggt
     1321 cgccgatcga ggtcgcgtgg tagccgcgct cgcagaagac ggcgatggcc ttgtccagca
     1381 cctcgtcgac gtcgaactcc cgcgggcgtc cacggccggg gctgtcggcg tgcggggcga
     1441 gggcggcatg ggcggcgggc atgggtgcca ttttgggaac attcgtttcc caattggcgc
     1501 gtgggggctt tggcgcggcg cgcgcctcgt cgatgcaatg gccccgcggg ccgggagggc
     1561 cgtgcgctca gctgcgcgcg cgcatggcgt tgcgcgcctg cacgaagccg aacgcgaact
     1621 cgacggcctc ggcgatcgag cgctcggact cggtgcgctc gcggtcggtc tggaagaacg
     1681 cgaagtcgac gtcgtggcgg atgtagcgcg ggggcagccg gttcagcgcg acgcaggcga
     1741 tgtcggccag catgtccggg ttgtccttga tgtgcgggaa gtgctccgcc atctcctgca
     1801 cggcggcgaa gacggccttc tcgtggttgt tgtggatcga tgcgatgtcc atggcggagc
     1861 ctgtcgggga gggggccgcc gcgcgggcgg gcggtcgttc ggccgagtgt gccgcgtcgc
     1921 gcgcggccgg tgtcgacaag tgtgcgacaa agtgccggct cgcgggcggc ccgctgccgg
     1981 cctcggccgt gcgcggggtc aggcgatctc gcccgtcacc cgcatcgaga agtcggtcgc
     2041 cgcgacgtcc ttggtgagct tgccgaccga gatgcggtcg acccccgtgg ccgcgatctc
     2101 gcgcagctgc gacagcgtga cgccgcccga gacctccagc accgcccggc ccttggcgac
     2161 ggccacggcc tcgcgcatgg cggcgaggct gaagttgtcc agcagcacgc tcgtcgcgcc
     2221 ggcgtccagg gcctcgcgca gctggtcgac ggactcgacc tcgatctgga tgtcgacccc
     2281 cgcgtccagc gcgagcgcct gcgccatcac ctgcgcgatg ccgcctgccg cggcgatgtg
     2341 gttctccttg atcaggatgc cgtgccacaa cgcgagccgc tggttgcggc cgccgccgac
     2401 ccgcaccgcg tacttctgcg cctggcgcag gccgggcagc gtcttgcgcg tgtcgagcac
     2461 ggcgcagccg cgggggctgg tggaggcgcc ctcgatcgcc tcgacatggc ggcgcgtgac
     2521 cgtcgcggtg ccggacagca gctgcaggaa gttcagcgcc gggcgctcgg ccgacagcag
     2581 cgcgcgggcg tcggcctcga tgcggcagac ctcggagtcg gccgtcatcg tcgcgccctc
     2641 gccgtacatc cagtcgatgc gcgcctcggg atcgagcgtc gacagcgtgg agcagaacca
     2701 gtcgcggccg cacagcacgg cgctttcgcg cacgatgacc gaggcctgca cgcgccggcc
     2761 ggcgggaatc agcaggctgg tccagtcggc gcggccgatg tcctcgaaca gcgcgtcgcg
     2821 cacgttgcgc gcgcgtgcct cggccagggt ttcgtcgtgg tcgaacatgt ctcctcgatt
     2881 cttctctcga aatgcgttca ggccgcgccg atgccgggca cgatgccggc ggcggcaggg
     2941 cggccggtgg cgtccttcgc ggccttggcg atggcggacg ggttggcggc gacgaagtcc
     3001 agcatgcggc ggatgcagcc ttcggcctgg gcgcgcacgg gctcggccac ctcgatcgcg
     3061 cccgtgtcgt gctccaggca gtcgaccagg ccctgcagcc cgttcatcgc catccacggg
     3121 caatgggcgc agctcttgca ggtggccgag ttgccggccg tgggcgcctc gatcagcgtc
     3181 ttgcccgggg cgagctggcg catgcgatgc aggatgccgt tgtcggtggc gacgatgtac
     3241 tccttcgcgc tgccgcggac gacggcgtcc agcatctgcg aggtcgagcc gacgacgtcg
     3301 gccatctcga ccacgctgcg cggcgactcg gggtggacga ggatcatcgc gtccggatgc
     3361 gcgcgcttca acagctcgag ctccaggccc ttgaactcgt cgtgcacgat gcaggcgccg
     3421 ttccacagga gcatgtcggc gccggtctgc tcctggatgt agcggccgag atggcggtcg
     3481 ggcgcccaga ggatcttctc gccgcgcgcc ttcaggtggc tcacgacggc gagcgcgcac
     3541 gagctggtga ccatccagtc ggcgcgcgcc ttcacggcgg cgctcgtgtt ggcgtagacg
     3601 acgacggtgc ggtcgggatg ggcgtcgcag aaggccgcga agtcctcggc ggggcagccg
     3661 aggtcgagcg agcaggtcgc gtcgaggtcc ggcatcagca cgcgcttggc gggcgacagg
     3721 atcttcgagg tctcgcccat gaagcgcacg cccgcgacga cgagggtgcg ctgcggcgcg
     3781 tcgcggccga agcgggccat ctcgagcgag tcggcgacgc agccgccggt ctcgagcgcg
     3841 aggtcctgca ggtcgccgtc gacgtagtag tgcgcgacga gcgcggcgtc gcgcgccttc
     3901 agcagttctt tggcgcgggc cttcaggcgc gcgcgttcgt cggggcccgg cacgggcagg
     3961 accttggccc aggcgctggc ggtgcaggtc gcgccctggg cgtcctggcg gtcgtattcg
     4021 taggtggctt gcgtcgtcat ggcggtcggc cgccggtgcg cggcatccgg caggtctcga
     4081 ggcgggccgt catcgtagcc caaggccgga gcgagcgacg ccagcaggga ctcgcgtccc
     4141 gcacgcgccg cgatcagctg ccgatccagg cttcgtagag gctgttccac acggtggtga
     4201 ccgagcggtt cagcggccgc gcgtgcaaca ccgtgggcgg cacgggccag tcgcgcgact
     4261 cgatctggct ctcgaggccg cggtggtcgg cgccgaggta gaacagccag cgcacgttgg
     4321 cggcgaaggg ctgcagcgcg aggcggtgca tctcgtcgac cagcgccacg gcgtcggccg
     4381 agggcatcgg cccgaccagc accgagcaga tctgcgagtg cgcgagcagg gcgccgaaga
     4441 cggcgcggcg atcggccggc cgggcgtcgc catgcaggca gcgcacgagg cggtgcggac
     4501 cgttgccggc aggcaacagc gtctcgtcga ccaccgcccg gatctgcagc ccgccgcgcg
     4561 aatgaacgcg cagccggtcg accggcgagc cggtggcgtc ggacagcgtc tggatcatgc
     4621 cgtgcgccca gacgcccgcg gggtcgacca cgagcatcgc gctgacctcc ggcgaggacg
     4681 ccaggtgcgc gtgcagcgcc agcgcgcctt gctcgctctc gggcacttcg accgtgacgc
     4741 ccagggcgcc ggccgagtcg aacatcgacg acgggcccga cggcggaaca tccttgaccg
     4801 gcaacaccag cggagcgagc tgctcgcgca gcgcgcgcaa cgcattgggc agtgtcagga
     4861 cggacggtcg gggcatggaa gacgagctta ctctggagga catcgcgggc cgcgggcaag
     4921 ttgtttaagg ttgtgacttc cttgtcgatc ttgcgaaatt cctggggccg acgcctgcat
     4981 tcacagggca cgtttcgcgc ccgccggccc tgcgcagcgg gcgcgcgcgc cccggcgccg
     5041 ggcttaccat cgcgggtcgt tccacgcgcc tcgcgcgccc cctttccgga tgccgtccat
     5101 gagcccgtcg cccgtcccgc ccgccctcgc gcccgcccgc ccccgcctcg ccggccatgc
     5161 cctcgtggag gcgctggtcg cgcagggcgt ggacaccgtc ttcggggtgc caggcgagag
     5221 cttcctcgcc gccctggacg gcttccacga gcacgccggc cgcatccgct tcgtcgcctg
     5281 ccgacacgag ggcggcgccg cgttcatggc ggaggcgcac ggcaagctct cgggccggcc
     5341 cggcgtctgt ttcgtgacgc gtggtcccgg cgccacgaac gcgagcatcg gcgtgcacgt
     5401 ggcgttccag gactcgacgc cgatggtgct gctggtcggc caggtcgccg cagaccagcg
     5461 cgatcgcgag gcgttccagg agctcgacta ccgccagatg ttcggccccg gcacgctggg
     5521 cctcgcgaag tgggtcggcg aggtgcagtc cgccgaccgc ctgcccgagt acgtcgcgcg
     5581 cgccttccgc acggcgatgc agggacggcc cgggccggtc gtgctcgcgc tgcccgagga
     5641 catgctggcc acgctcaccg ccgcgccggt gctgccgcgc gtcgagcccg cacgcgcgtg
     5701 gcccgagccg caggccgtcg agcgcctgcg ccagctgctg atccgcgcgg agcggccgat
     5761 cgcgatcgtc ggcggcagcg gctggacggc cgacgcggcg tgggagctcg agcgcttcgc
     5821 cgaggcctgg gagctgccgg tggcctgcgc gttccgcttc caggacacct tcgacaaccg
     5881 ccaccgcctg tatgcgggcg acgtgggcat cggtatcgcg ccggcgctcg cgcagcgcat
     5941 ccgcgacgcc gacctcgtgc tggcgatcgg cgtgcgcctg ggggagatga ccacgggcgg
     6001 ctactcgctg ctgcaggcgc cggtcgcgcg ccagccgctc gtgcacatcc acgccggcgc
     6061 cgaggagctc ggacgcgtct accagccgac gctcgcgctg cagtgcgcga tggcgggcgt
     6121 cggcggcgtg ctcgcggagc tggatccgcc ggctccgcgc tcgccgcggg ccggcgcgca
     6181 ggcgcatgcg gactacctcg ccaacctcga ggccgcggcc gtcgagccga tggatctcgc
     6241 ggcggtcgtg aagacggccg gggccctcgc gccggcgggc accgtgtgga cgaacggcgc
     6301 gggcaactac gcgggctggc tgcaccgctt ccatccctac gagggcctgc agcatcgggg
     6361 ccgcacgcag ctcgcgccga ccgcgggcgc gatgggctac gccgtaccct cggccgtcgc
     6421 ggcgagcctg ctgcagccgg accgcacggt ggtcggcatc tcgggcgacg gcgacttcct
     6481 catgaccgcc caggagctcg cgaccgcgac cgcgcacggt gccggccggg gtgccgggcg
     6541 gctcgtggtg gtggtcgtcg acaacggcca gtacggcacg atccgcatgc accaggagcg
     6601 cgagttcccg gcccgcgtga gcggcaccgc gctcgccaat ccggacttcg tcgcgctcgc
     6661 gcatgccttc gactgggcgg ccgagcgcgt cgacgacacc gccgccttcg agcccgcgtg
     6721 gcgacgcgcg ctcgccgccg accgtccgac gctcctgcac ctgatcctgc ccgccgaccg
     6781 catcacctcg cgcaccacgc tcgcagcgct gcgcgaggcc gcgctggcgc acggcgctgc
     6841 atcgggcggt taggcgcgcg gcgccgcatc ccgcggggcg agggcgtccg cggccgcacg
     6901 gtagagcagc cttgcacgac aggcacgttt gcgcggcgtt gtcctacacg ccaggacgcg
     6961 gtgccgggcc gatactgcga tctcccggcg gcgctacctc ctccctccct cctctttggt
     7021 cgccggtctc agccccgcca tgtgcggggc ttttttcttg tcggagacga tgctgcccgc
     7081 tccggcgatg gttctccggg ctccggcgtc cgcccccagc gctccggcgc gcgcccgcgg
     7141 agcttcgccc tcctgcgttt gcgaggcatc gccggcgttc cgaagcgcga ccgcgcctcg
     7201 cacaggctcg tcctcccggc tccgcgagca tgcgccggag ctccgcaagg aagccgccgg
     7261 agttcggcga cgaaacgccg gagctccgca ggaaggcgcc ggagctccgc aggaaggcgc
     7321 cgaagctccg cgggaaggcg ccgaagctcc gcaggaagac ggccaagctc cgcactaagg
     7381 cgccgaagct ccgcgacgaa gcgccggagc tgcgcgaagg gacgccggag ccggagaccc
     7441 ctcgacggcg ccggcgttgc ggccgccgac ctgccgggcc gccgcgtttc gcgggcgccg
     7501 gcgatcagac atccagcgcg tcgatgctct tgccgacgtc gcgctggacg gcagcctggg
     7561 gatcgccgcc gcgctccggg tcgggcaggg cgaggccgcc gccggccagc gtgcgcaccc
     7621 cgctgcgcag cgacaccgcc gcttgccacg acaaggcctc gtccgcgatc gtccacgtcg
     7681 ccacgaaccg gatcttggcc ttcgtgacgg cgtggcgatg gatgctctcg aagacgtgct
     7741 tcatgcgcgg cccgcgggcg agcttcgggt gcgcgcagtg tatgtggcaa gccaccgttg
     7801 cgggtgccgg agtgggcggc ggcctgcgcc tggcaggcaa gcacgccgag atcgacaagc
     7861 acccccgaca ggagtcttgt ccctgtcggt cacttgtatg gtcggggcga cacgattcga
     7921 acgtgcgacc ctctgctccc aaagcagatg cgctaccagg ctgcgctacg ccccgaagtc
     7981 cgggattcta cgtcctcggg ccgcggccga ggatgtcggc gaggaggcga gcgtcgagat
     8041 ggcgttcgac caggtcggcg aggcgatcga aggtggcgtc cagcgcgggg acggacgcgc
     8101 cgaacagcgc gcgcaacagg gcgggcgact cgagcaggcc gtgcgcatag acgcccagca
     8161 cgcagccgcg ctgccagccg atcggggcac cgccggcgtc gcgcagcgcc accgtcggcg
     8221 cggccatgtc gggatgcggc acggtgacgc cgcaatggat ctcgtagccg tcggcctcgc
     8281 agccggccca cggcgcccac gggccctcca gcggctcgaa gcgcaggcgg gcgcgacgca
     8341 cgcgcttgtc ggcctcgtag cgcgtcacga gcggcagcag ccccagcccc gggcccgcga
     8401 accccggctc gccgtcgtgg ccctcggggt cgacgagcgc ttcgcccagc atctgcaggc
     8461 cgccgcagac gcccagcacg ggccgaccgg cggccgcatg gcgggcgaca gccgcatcca
     8521 ggccctgcac gcgcatccat gcgaggtcgc cgctgacctg cttggagccc ggcagcacga
     8581 tccagtcggc gtccccgagg tcctgcagcc cgcgcaccca cgcgagctgc acgccgggca
     8641 ggcggcgcag cggctcgaac tcgtcgaggt tgctgaggtg cggccccgcg atcaccgcga
     8701 cgcgccgtcg ccccgtcttg ggcgcgagcg ggtcgccggc atcgtgcacg ccgtcctcgt
     8761 cgggcaggcc gtggtcgcgc accatcggga tggtgccggc cagcgccacc ccggtcagct
     8821 gccgcagctg cgcggggccg ggctccagca gcgccgcgtc gccgcggaag cggttgagga
     8881 cgaagccgcg cagcgtcggg cgcaggtccg cgggcagcag cgcccaggtg ccgtagaggt
     8941 gcgcgaaggc cccgccgcgg tcgatgtcgg ccaccagcag gctcgcgagc tcgcccaggc
     9001 cgcgcgcctg gcgtgctgtc gcgaggttga cgtagtcctg cggcgcgagg ttgatctcgg
     9061 ccggcgagcc ggcgccctcg atcacgacga gctcgcagcc gccggccagc cgcgccaggc
     9121 tctgcgccgc gaagcccgcg agggccgcgc tgcgctcgcg ccagccctgg cgcgacagcg
     9181 cggcatcgac gcggccgtga acgaccacct ggctcgcggt gtcgcgttcg ggcttgagca
     9241 gcaccgggtt catgtccacg gtcggctcga cgcccgcggc cagcgcctgg aaccactgcg
     9301 cggcgccgat ctccgcgccg cggccgtcca ggcccgcgac gacgcgcgcg ttgttgctca
     9361 tgttctgcgc cttgaacggc cgcacgtcga tgccgcgccg cgcggcgacg cggcacagcg
     9421 ccgtcgcgag ccagctcttg ccggcgccgc tggaggtgcc ccagcacatc acgaagcgcg
     9481 tcatcgcgcg tccccggcct cgagcgccag ccgctcgaac acgaaggtcg tcggcgcgaa
     9541 cggcggccgc gaggcgacgc gccgctgccc gagcgagcgc agccgcagcg gcgcggcgaa
     9601 gcccgggccg tcctcgacga aggtgtggaa ctcgccgtcc tcgccgaccg ggcatacggt
     9661 gttcggtatg tcggcgagga aggccgcgtc gtaggcgcgg ccgcagaacg cggcgtctag
     9721 gcgggcggtg tccacgcaca cgacgcgcgc acgcacgccg cgatccagca gctcgcgcgc
     9781 gagcgccgcg cgcggctcgc cccacagcgg gaagcgcgc
//
LOCUS       DASVLN010000458         7078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_508678, whole genome shotgun sequence.
ACCESSION   DASVLN010000458 DASVLN010000000
VERSION     DASVLN010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508678"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1009)
                     /locus_tag="VF453_11095"
     CDS             complement(32..1009)
                     /locus_tag="VF453_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688247.1"
                     /translation="MTLRAEFDKIELHLVRVLHTLLTERSVSRAALRLQSSQPAVSAQ
                     LKRLRELTGDPLLVRAGNGMTPTATGQSLLGPAANLLAEADRLFSPRARRDVFDPQTS
                     AATFRIAASDFIDPGFLPGLVLHLKALAPRVRIELQPLSSHFDYRRSLAQGEVDLVIG
                     NWLAPPGELHLGRLLNDEVVCLVSEDHPVARAGGGRAWTVEKYLACEHVAPMPNYQGA
                     RGVIDDHLRKLGLLRDITVRASHFSLIPPMVAGSRLVLTTGRLFCSRYVGALPVRIVR
                     CPVAFPPLAYYQLWHELTHESAPVRWLREQVRDVARGLAKPARAGGRAA"
     gene            1263..1721
                     /locus_tag="VF453_11100"
     CDS             1263..1721
                     /locus_tag="VF453_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7688248.1"
                     /translation="MDSIDLRILEILQVDGEAQVAHVAERVGLSTTPCWRRIQRMKEA
                     GVITRQVALVDPRAVNVGVTVFVSVRTSVHSQKWFDRFRATVHAIPEVVEFYRMSGEV
                     DYLLRVVVPDIAAYDAVYKRLTAGTELFDVSSSFAMEEIKFTTALPLSYA"
     gene            1812..3983
                     /locus_tag="VF453_11105"
     CDS             1812..3983
                     /locus_tag="VF453_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7688249.1"
                     /translation="MSLPTLLRPALVAVLVAGASAAAAQDAAAPASASAPAPAAAAST
                     SSAEDPYIWLEKVDSPEAMAWVRAENRKTLDELERDPRFQTYHQEALAIAQNRDRIPM
                     ASQVDGRIFNFWQDADHVRGIWRATSPADYDAGGTPAWKTVLDLDKVSADEHANWVWK
                     GARCEARRESRCLVQLSDGGEDAVTVREFDLRKPGFVKGGFMLPKGKQDLTWETPDSL
                     LVAREWHPGELTTSGYAYIVKRVRRGQPLSAAREIFRGKPSDVEVQLFTLDDARGHKA
                     VLINRGITFFESENWLVGPKGLQKLALPAHVDLLDLYDGQLIVKVKEDWTSGGVAVKE
                     GSIVGIDLAQARRHPDALEPTVVFEPTPRQTIEAVASTKDALVVSYLDNVRGRTALVT
                     RAPGGRWQRQALELPDNADVRVADTNLHTSTAYISVAGYLQPSSLWRADTAHAAVRQI
                     RAVPPQFDASKDVVEQFEATSSDGTKIPYFIVHPKDMALDGSNPTILYAYGGFEISLS
                     PSYSAVMGKLWLEHGGVFVVANIRGGGEFGPAWHDAGLKTKRQIVYDDFAAVGRDLVA
                     RGITSPRRLGIEGGSNGGLLMGVEMTEHPDMWNAVDIQVPLLDMLRFEQIAAGASWVG
                     EYGSVSVPAQRDFLAKISPYANLHRDVKYPKALVWTTTKDDRVGPQHARKFAARLSEY
                     GIPYYYYEVIEGGHGAGANLKERAHTSAIEYVYFTRQLMDK"
     gene            complement(4134..4271)
                     /locus_tag="VF453_11110"
     CDS             complement(4134..4271)
                     /locus_tag="VF453_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688250.1"
                     /translation="MESLRHERRWQLEALAWALAVLACDVLLRAAGLPPARSGTSCWC
                     G"
     gene            complement(4274..4660)
                     /locus_tag="VF453_11115"
     CDS             complement(4274..4660)
                     /locus_tag="VF453_11115"
                     /inference="COORDINATES: protein motif:HMM:NF040085.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HEX7688251.1"
                     /translation="MAAEVQTRAMMSSKIDSAVRQAIAAGIRPFDAAPPLYRLAWRLG
                     LDLRPPLYASFAVNALVMGGFYGASMGVLGLLWAMSRPGWRPALALPVLGLACAAGAI
                     FGILMAGLMRRRAGRAGLSSWDRLEA"
     gene            complement(4805..4881)
                     /locus_tag="VF453_11120"
     tRNA            complement(4805..4881)
                     /locus_tag="VF453_11120"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4845..4847),aa:Met,seq:cat)
     gene            complement(4964..5674)
                     /locus_tag="VF453_11125"
     CDS             complement(4964..5674)
                     /locus_tag="VF453_11125"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEX7688252.1"
                     /translation="MRLAQILFSQGFGTRRACEGLAAAGLVAIGGVVRDDPFEDLEPE
                     GLVLTVEGGDWPYHAKALVALNKPAGYECSRKPRHHASVYSLLPAPLLRRDVQAVGRL
                     DEDTTGLLLFTDDGQLLHRWTSPRHHVPKVYDVSCRHAVTDEQLERLRAGVVLQDDPE
                     PVRALAAESWNGAAAGLHGVRLTLAEGKYHQVKRMMAAVGNRVEALHRSRFGRLELPA
                     DLAPGTWCWLPGTHVVAG"
     gene            complement(5671..>7078)
                     /gene="dsbD"
                     /locus_tag="VF453_11130"
     CDS             complement(5671..>7078)
                     /gene="dsbD"
                     /locus_tag="VF453_11130"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEX7688253.1"
                     /translation="GDLLGDAGATPAANPVTGLPQAAATVVVAPAAVADDADGGIGAA
                     LAGGHLLAIVAVAVAIGLGLSATPCVWPMFPILSSVIVGQGNRVTPARGLGLAAAYAL
                     GMALVYTLLGVAAGLVGKGFTDTLQKPAVLLAFGALLVVLAASMFGLYELQLPAALRD
                     RLSARSDRLSGGQVGGVFGMGVLSALLVSPCVTAPLFSVLLFIAQHQAPVLGGIALFC
                     IALGMSVPLLLIGASAGALLPRSGPWMEQVKRAFGLVLLGVALYIVRSVLPAAVVMLG
                     WGVLAIVGGVVLGAFEPATSEAYPGAARTVKGFGILFVVIGALELVGVASGGTDPARP
                     LARLAQARAGAAAAEAGPRFDRVASVAELDQRLQSAGRPVMLDFYADWCTSCKEMEQD
                     TFADPAIAAKLGRAVLLRADVTASTPQDVALLKRFGLFGPPGLIFFDAQGRELPTARV
                     VGFQAPERFARSLAAAGL"
BASE COUNT         1124 a         2581 c         2490 g          883 t
ORIGIN      
        1 gggcggggcg cggtcatcga agcggcgatt gtcacgcggc gcggccgccg gcgcgcgccg
       61 gcttcgcgag cccccgcgcg acgtcgcgca cctgctcgcg cagccagcgc accggcgccg
      121 actcgtgcgt cagctcgtgc cagagctggt agtaggccag cggcgggaac gccaccgggc
      181 agcgcacgat gcgaaccggc agcgcgccga cgtagcgcga gcagaacagc cgcccggtcg
      241 tcagcacgag gcggctgccc gccaccatcg gcgggatcag cgagaaatgg ctcgcgcgca
      301 cggtgatgtc gcgcagcagg ccgagcttcc gcaggtggtc gtcgatcacg ccgcgcgcgc
      361 cctggtagtt gggcatcggg gcgacatgtt cgcaggcgag gtatttctcg accgtccagg
      421 cgcgtccgcc gccggcgcgc gcgaccgggt gatcctcgga caccaggcag acgacctcgt
      481 cgttcagcag ccggcccagg tgcagctcgc ccggcggcgc gagccagttg ccgatgacga
      541 gatcgacctc gccctgcgcg aggctgcggc ggtagtcgaa gtgactcgag agcggctgca
      601 gctcgatgcg cacgcgcggc gcgagcgcct tcaggtgcag caccaggccg ggcaggaagc
      661 cggggtcgat gaagtcgctc gcggcgatgc ggaaggtggc cgcgctcgtc tgcgggtcga
      721 agacgtcgcg gcgcgcccgc gggctgaaca ggcggtccgc ctcggcgagc aggttcgcgg
      781 ccggccccag cagcgactgc ccggtcgccg tcggcgtcat gccgttgccc gcccgcacca
      841 gcagcggatc gcccgtgagc tcgcgcagtc gcttcagctg cgcgctgacc gccggctgcg
      901 agctctgcag gcgcagcgcc gcgcgcgaga cgctgcgctc ggtcagcagc gtgtggagca
      961 cgcgtacgag gtggagctcg atcttgtcga actccgcgcg cagcgtcatg cggcccggcg
     1021 aatggtccat atgcgctcga ttatatggac ggggtcggct ggaaaggcgg cgaggcggcc
     1081 cctccccgga cccgcctcgc cgtgtgcaac cagtggacgc gatgtccccg gggccccaac
     1141 cgtgccggcc gatggccgcg cgcggacatt gtccgcacga cggcgtggaa tcgtttgcca
     1201 agacggccgc cgttctcgca caatcgcgaa atatcatgcc aagcaaaccg tcttccataa
     1261 aactggattc catcgacctg cggatcctgg agatcctgca ggtcgacggg gaagcgcagg
     1321 tggcgcatgt cgccgagcgc gtcggcctgt cgacgacgcc gtgctggcgc cgcatccagc
     1381 gcatgaagga ggccggcgtg atcacgcgac aggtggcgct cgtcgacccg cgcgcggtga
     1441 acgtgggcgt gacggtgttc gtgtcggtgc gcaccagcgt gcactcgcag aagtggttcg
     1501 accgcttccg ggcgacggtg cacgcgattc ccgaggtcgt cgagttctac cgcatgagcg
     1561 gcgaggtcga ctacctgctg cgcgtcgtcg tgcccgacat cgccgcctac gacgccgtct
     1621 acaagcgcct gaccgcgggc acggagctgt tcgacgtcag ctccagcttc gcgatggagg
     1681 agatcaagtt cacgaccgcg ctgccgctgt cgtacgcgtg acggcccctc gcgacgccag
     1741 ggaatgaccc ggtcgggccg gcgccgtggc ggtggcaaca tcgcgccttc cccgaaacag
     1801 gaaagaccgc gatgtccctg cccaccctgc tccgtccggc cctcgttgcc gtgctggtcg
     1861 ccggcgccag cgccgccgcc gcccaggacg cggccgcgcc ggcgtccgct tccgcgccgg
     1921 cacccgccgc ggctgcgtcc acctcgtccg ccgaggatcc ctacatctgg ctcgagaagg
     1981 tcgacagtcc cgaggcgatg gcctgggtgc gcgccgagaa caggaagacg ctggacgagc
     2041 tcgaacgcga cccgcgcttc cagacctacc accaggaagc cctcgccatc gcgcagaacc
     2101 gcgaccgcat cccgatggcc tcgcaggtcg atggccgcat cttcaacttc tggcaggacg
     2161 ccgaccacgt gcgcggcatc tggcgcgcga cctcgcccgc cgactacgac gccggcggca
     2221 cgcccgcctg gaagacggtg ctggacctcg acaaggtctc ggccgacgag catgccaact
     2281 gggtgtggaa gggcgcgcgc tgcgaggccc gccgcgagag ccgctgcctc gtgcagttgt
     2341 ccgacggcgg cgaggacgcg gtcacggtgc gcgagttcga cctgcgcaag cccgggttcg
     2401 tcaagggcgg cttcatgctc cccaagggca agcaggacct cacctgggag acgccggaca
     2461 gcctgctcgt cgcgcgcgaa tggcatccag gcgagctgac gacctccggc tacgcctaca
     2521 tcgtcaagcg cgtgcgccgc ggccagccgc tgtcggccgc cagggagatc ttccgcggca
     2581 agccgtcgga cgtcgaggtg cagctgttca cgctggacga cgcgcgcggc cacaaggcgg
     2641 tgctgatcaa tcgcggcatc accttcttcg agagcgagaa ctggctcgtc ggccccaagg
     2701 gcctgcagaa gctggcgctg cccgcgcacg tcgacctgct cgacctctac gacggccagc
     2761 tgatcgtcaa ggtcaaggag gactggacgt ccggcggggt cgcggtcaag gaaggctcca
     2821 tcgtcggcat cgacctcgcg caggcgcgcc gccacccgga tgcgctcgag ccgacggtgg
     2881 tgttcgagcc gacgccgcgc cagacgatcg aggccgtcgc gtcgacgaag gacgcgctgg
     2941 tcgtctccta tctcgacaac gtgcgcggtc gcacggcgct cgtcacgcgc gcgcccggcg
     3001 gccgctggca gcggcaggcg ctcgagctgc cggacaacgc cgacgtgcgc gtcgccgaca
     3061 ccaacctgca cacctcgacg gcctacatct cggtagccgg ctacctgcag ccgtccagcc
     3121 tgtggcgggc cgacaccgcg cacgccgcgg tgcggcagat ccgcgcggtc ccgccgcagt
     3181 tcgacgcgtc gaaggacgtc gtcgagcagt tcgaggcgac ttcgtccgac ggcaccaaga
     3241 tcccgtactt catcgtgcac ccgaaggaca tggcgctgga cggcagcaac ccgaccatcc
     3301 tgtacgccta tggcggcttc gagatctcgc tgtcgccgtc ctacagcgcg gtcatgggca
     3361 agctgtggct ggagcacggc ggcgtcttcg tggtcgccaa catccgcggc ggcggggagt
     3421 tcgggcccgc gtggcacgac gccggcctga agacgaagcg ccagatcgtg tacgacgact
     3481 tcgccgccgt gggtcgcgac ctcgtggcgc gcgggatcac gagcccgcgc cgcctcggca
     3541 tcgagggcgg ctccaacggc gggctgctga tgggcgtcga gatgaccgag caccccgaca
     3601 tgtggaacgc ggtcgacatc caggtgccgc tgctggacat gctgcgcttc gagcagatcg
     3661 cggccggcgc gtcgtgggtg ggcgagtacg gctcggtctc ggtgcccgcg cagcgcgact
     3721 tcctcgcgaa gatctcgccc tacgccaacc tccaccgcga cgtgaagtac ccgaaggcgc
     3781 tcgtctggac gaccaccaag gacgaccgcg tcgggccgca gcacgcgcgc aagttcgcgg
     3841 cgcggctgtc ggaatacggc atcccgtact actactacga agtgatcgag ggcggccacg
     3901 gcgccggggc caacctgaag gagcgcgcgc acacgagcgc gatcgagtac gtctacttca
     3961 cgcgccagct gatggacaag tgaggccgcg tcgcagttcg cgcatcggcg cggaccgcgg
     4021 tgatcaggcc cgcgcgcggc gggacttcgg cgccgacctc gcgcgcggcg ccatcgcctt
     4081 cacgcccagc gccagcacga acgaggtcgc gcgctgcagg cccgtcgcgc agatcagcca
     4141 caccagcagc tcgtgccgga gcgcgccggc ggcagcccgg ccgcgcgcag caggacgtcg
     4201 caggccagga cggccaacgc ccaggcgagg gcctcgagct gccagcggcg ctcgtgtcgc
     4261 agcgactcca tcgtcaggcc tccagccggt cccaggacga caggcccgcg cgccctgcgc
     4321 ggcggcgcat caggcccgcc atcaggatgc cgaagatcgc gcccgccgcg caggcgaggc
     4381 cgaggaccgg cagcgccaac gccgggcgcc agccggggcg gctcatcgcc cagagcagcc
     4441 cgaggacacc catggacgcg ccgtagaagc cgcccatgac gagcgcgttg accgcgaagc
     4501 tcgcgtacag cggcggacgc aggtccagcc ccagccgcca cgccagccga tacaacggcg
     4561 gcgccgcgtc gaacggacgg atgcccgcgg cgatggcctg gcggacggcg gaatcgatct
     4621 tcgaggacat catggcgcgg gtctggactt cggcggccat gctagctcga ccgtggcgcc
     4681 gacgcgtccc ccaaaagggt cgtcgacagg ctcgtgacag cgcgcaccgc gccgcaagcc
     4741 ggcccaaaag caaaaaaccg cgggaagcag ggcttcgcgc ggttccgctg gtcagtgacc
     4801 gaaatggttg cgggggctgg atttgaacca acgacctttg ggttatgagc ccaacgagct
     4861 accagactgc tccaccccgc ggcaagaaag tgattatggc acgaaacaat gtcacgtgtc
     4921 cacctctgga tcgcgataac gcgcaaatcg ccgcggcggc cgttcagccg gcgacgacgt
     4981 gcgtcccggg cagccagcac caggtgccgg gggcgaggtc ggccggcagc tccaggcgcc
     5041 cgaagcgact gcggtgcagt gcctccacgc ggttgccgac cgccgccatc atgcgcttga
     5101 cctggtggta cttgccctcg gcaagcgtca ggcgcacgcc gtgaaggcct gccgcggcgc
     5161 cgttccagga ctcggcggcg agcgcgcgca ccggctccgg atcgtcctgc agcacgacgc
     5221 ccgcgcgcag gcgctcgagc tgctcgtccg tcaccgcgtg gcggcacgac acgtcgtaga
     5281 ccttcggcac gtggtgcctg ggcgaagtcc agcgatgcag cagctggccg tcgtcggtga
     5341 acagcagcag gcccgtcgtg tcctcgtcga gccggccgac cgcttgcacg tcgcggcgca
     5401 gcagcggcgc cggcaacagg ctgtagacgc tcgcgtggtg gcgcggcttc ctcgagcact
     5461 cgtagcccgc cggcttgttc agcgcgacga gcgccttcgc gtggtacggc cagtcgccgc
     5521 cctcgacggt gagcacgagg ccctccggct cgagatcctc gaacgggtcg tcgcgcacca
     5581 ccccgccgat cgcgaccagc cccgccgcag ccaggccctc gcatgcgcgg cgggtgccga
     5641 agccctgcga gaacaggatc tgcgcgagcc tcacagccct gccgccgcga ggctgcgggc
     5701 gaaacgctcc ggcgcctgga agccgacgac gcgcgcggtc ggcagttcgc ggccctgggc
     5761 gtcgaagaag atcagcccgg gcggcccgaa cagcccgaag cgcttgagca gcgccacgtc
     5821 ctgcggcgtg ctcgcggtga catcggcgcg cagcaggacg gcgcggccca gcttcgcggc
     5881 gatcgcggga tcggcgaagg tgtcctgctc catttccttg caggaggtgc accagtcggc
     5941 gtagaagtcg agcatcaccg ggcgcccggc ggattgcagt cgctggtcga gctccgcgac
     6001 gctcgcgacg cggtcgaagc gcgggccggc ctcggccgcg gcggcgcccg cacgcgcctg
     6061 cgcgaggcgt gcgagcgggc gggcgggatc ggtgccgccg ctggccacgc cgaccagctc
     6121 cagtgcgccg atcacgacga acaggatgcc gaagcctttc accgtgcgcg ccgcgcccgg
     6181 ataggcttcg ctcgtcgcgg gctcgaacgc ccccagcacg acgccgccga cgatcgcgag
     6241 cacgccccag cccagcatca ccacggccgc aggcagcacg ctgcggacga tgtacagcgc
     6301 cacgcccagc agcacgaggc cgaacgcgcg cttgacctgc tccatccacg ggccgctgcg
     6361 cggcagcagg gcgcctgccg aggcgccgat cagcagcagc ggcacgctca tgccgagcgc
     6421 gatgcagaac agcgcgatgc cgcccagcac cggcgcctgg tgctgggcga tgaacaacag
     6481 caccgagaac agcggcgccg tcacgcacgg gctcacgagc agcgccgaca gcacgcccat
     6541 gccgaacacg ccgcccacct ggccgccgga caggcgatcg ctgcgtgcgc tcaggcgatc
     6601 gcggagggcg gcgggcagct ggagctcgta gagaccgaac atcgacgccg ccagcaccac
     6661 cagcagcgcc ccgaaagcca gcaggacggc cggcttctgc aacgtgtcgg tgaagccctt
     6721 gccgaccagg cccgccgcca cgcccagcag cgtgtagacc agcgccatgc ccagcgcgta
     6781 cgccgccgcc agcccgagcc cgcgcgccgg cgtcacccgg ttgccctggc cgacgatcac
     6841 cgacgacagg atcgggaaca tcggccacac gcagggggtg gccgacagcc cgaggcctat
     6901 cgccacggcc accgcgacga tggccagcag gtggccgccc gccagcgcgg cgccgatgcc
     6961 gccatcggca tcgtcggcga ctgccgcagg cgcgacaacg accgtcgcgg ccgcctgcgg
     7021 caggccggtc accggattgg ccgccggcgt cgcgccggcg tcgcccagca ggtcgccg
//
LOCUS       DASVLN010000459         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_454282, whole genome shotgun sequence.
ACCESSION   DASVLN010000459 DASVLN010000000
VERSION     DASVLN010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454282"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VF453_11135"
     CDS             complement(<1..1256)
                     /locus_tag="VF453_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7688254.1"
                     /translation="MTPRPGPPAPPGDPPARAVDARDRFLFEHSPLPMWIYDLATLRF
                     LDVNEVACTNYGWTREEFLAMTIRDIRPPEDLDALAESVRTQPAQVFHAGVWRHRRKD
                     GSVIAVEITSHELLFDGRRTRMVCPIDVTARVRAEAALLEREAALRRAQSLAGLGHAV
                     SRPDGSFESWSESLPSLVALPAERMPASTRDWLRLIHPDDRAMFRDRSLEAASVGVRV
                     DVEYRLVRGDGRTIHMRQVIEPIRDAAGAPTGRWFSTVQDVTAQKTAEVAARRANEAL
                     EERVRERTEQLEASNVELAFATADAERANRAKSEFLSNMSHELRTPLNAIIGFGQLLA
                     MPEMDGRNDAQRRVFVDHIVDAGRHLLTLINEILDLAQVEAGRMEVRAERVELAPLLA
                     ECGAMLAPLASQRGVAVRLDAAPGLAL"
     gene            1444..>2109
                     /locus_tag="VF453_11140"
     CDS             1444..>2109
                     /locus_tag="VF453_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688255.1"
                     /translation="MTSPVTRRLARTLWTDDRHTRIRLQRSMLSFALYLECALGMGLL
                     TRWGMLAGRDILAWTAWVVAGLGGFYALIRSGWSRRLADPSMSVAQIAFGLGSGVFGY
                     WIGGAARSATLMPLFLIMIFGAFSISQRQMVALTALALVALAATMLARHARHAGAGID
                     VLDLSNFVMVSIVLPGIAMVTILLGRLRKRMAAQREDLRAALARIQELATLDELTGLA
                     NRRR"
BASE COUNT          255 a          773 c          774 g          307 t
ORIGIN      
        1 agcgcgaggc cgggcgcggc gtccagccgc acggcgacgc cacgctggct cgccaggggc
       61 gccagcatcg cgccgcactc ggccagcagc ggcgcgagct cgacgcgctc ggcgcgcacc
      121 tccatgcggc cggcctcgac ctgcgcgagg tcgaggatct cgttgatcag cgtcagcagg
      181 tggcggccgg cgtcgacgat gtggtcgacg aacacgcggc gctgcgcgtc gttgcggccg
      241 tccatctcgg gcatcgcgag cagttgcccg aatccgatga tggcgttcag cggcgtgcgc
      301 agctcgtggc tcatgttgga gaggaactcc gacttcgcgc ggttggcgcg ctcggcgtcg
      361 gcggtcgcga aggcgagctc gacgttggag gcctccagct gctccgtgcg ctcgcgcacg
      421 cgctcctcga gcgcctcgtt cgctcgccgc gcggcgacct ccgcggtctt ctgcgcggtc
      481 acgtcctgca ccgtgctgaa ccagcggccg gtcggcgccc cggcggcgtc gcggatcggc
      541 tcgatcacct ggcgcatgtg gatcgtgcgg ccgtcgccgc gcacgaggcg gtactcgacg
      601 tccacgcgca cccccacgct cgcggcctcg aggctgcggt cgcggaacat cgcgcggtcg
      661 tcgggatgga tcagccgcag ccaatcgcgc gtcgaggcgg gcatgcgctc ggccggcagc
      721 gcgacgagcg agggcagcga ctccgaccag ctctcgaacg acccgtccgg ccgcgagacc
      781 gcatgcccga ggccggcgag cgactgcgcg cgccgcagcg cggcctcgcg ctcgagcagc
      841 gccgcctcgg cccgcacgcg cgcggtgacg tcgatcgggc acaccatgcg cgtgcgccgg
      901 ccgtcgaaca ggagctcgtg cgaggtgatc tccaccgcga tgaccgagcc gtccttgcgg
      961 cgatggcgcc agacgcccgc atggaacact tgggccggct gcgtgcgcac cgactcggcc
     1021 agcgcgtcga ggtcctccgg cggccggatg tcgcggatcg tcatcgcgag gaattcctcg
     1081 cgcgtccagc cgtagttggt gcacgcgacc tcgttgacgt ccaggaagcg aagcgtcgcc
     1141 aggtcgtaga tccacatcgg cagcgggctg tgctcgaaga ggaagcggtc gcgcgcgtcc
     1201 acggcccgcg ccggcgggtc gccgggcggc gcgggcggac cggggcgggg cgtcatggcg
     1261 tcgttctact cccttgccgc gggggcgtca ccgtgcttgg gcagtcggcg cgcccgcggc
     1321 ggggccactg tccggcaagg cgcccgccaa gtccagttgc agttcacgcg ccgatgcgtg
     1381 cggagttctc gaccgccgcc ggaacggcgc tacagccgcg cggcccgcgc ccgataattc
     1441 cccatgacct cgcccgtgac gcgccggctc gcccggacgc tgtggaccga cgaccgccac
     1501 acgcggatcc ggctgcagcg ctcgatgctg agcttcgcgc tctacctgga gtgcgcgctc
     1561 ggcatgggcc tgctgacccg gtggggcatg ctggccggcc gcgacatcct cgcgtggacg
     1621 gcctgggtcg tcgccggctt gggcggcttc tacgccctga tccgcagtgg ctggagccgg
     1681 cgcctggccg acccgtcgat gagcgtcgcg cagatcgcgt tcggcctcgg ctcgggcgtc
     1741 ttcggctact ggatcggcgg cgcggcgcgc agcgcgacgc tgatgccgct gttcctgatc
     1801 atgatcttcg gcgccttctc gatctcgcag cggcagatgg tcgcgctgac ggccctggcg
     1861 ctcgtcgcgc tcgcggcgac gatgctggcg cggcacgcgc ggcatgccgg tgcgggtatc
     1921 gacgtgctcg acctgtcgaa cttcgtcatg gtctcgatcg tcctgccggg catcgcgatg
     1981 gtgacgatcc tgctgggccg cctgcgcaag cgcatggccg cgcagcgcga ggacctgcgc
     2041 gccgcgctcg cgcgcatcca ggagctcgcg acgctcgacg agctcaccgg cctcgccaac
     2101 cgccgccgc
//
LOCUS       DASVLN010000460        48412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_23784, whole genome shotgun sequence.
ACCESSION   DASVLN010000460 DASVLN010000000
VERSION     DASVLN010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 48412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48412
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23784"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..582)
                     /locus_tag="VF453_11145"
     CDS             complement(247..582)
                     /locus_tag="VF453_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688256.1"
                     /translation="MPHDITDVSSIPPASMFLAGRNLALVVLRELFAVGSDRCSIEAE
                     QRPGVAQCDALARAIESLRTAPSQVVSGFAAVFTDVAGCDCSATPEHFELLTPAEMGF
                     ETSAPTFRH"
     gene            complement(1321..2550)
                     /locus_tag="VF453_11150"
     CDS             complement(1321..2550)
                     /locus_tag="VF453_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7688257.1"
                     /translation="MTALAVSRLSAPGLYFVGEVPGLALQVLETGGRTWVLRVTIGGK
                     RRDMGLGGYPAVTLAGAREAARAARLKVRQGVDPIEEARASKSALRAARASALSFDEC
                     AAKYIKANAAKWKNAKSGQQWRNTLETYAGPVMGNLLVRDVGMSHVLAVLEPIWRTKT
                     ETASRLRGRIEAVLDWATACGYRDGLNPARWKGHLAKVLPAANEVRGKDHHPALPIDE
                     MGGFMLRLRQSEGMGARALEFAILTAARSGEVRGAVWAEFDFDEAVWNVPAERMKGRK
                     PHRVPLSPTAVTLLKSLPKRTDSPYVFAGRGAGTLSDMTLLSVVRRLDVPVVPHGFRS
                     TFRDWAAERTNYPNHVAEMALAHTIGDKVEAAYRRGDLFQKRVRMMTDWAKFCGKVEQ
                     KGVVVPMNPKERRKTAA"
     gene            complement(2555..3499)
                     /locus_tag="VF453_11155"
     CDS             complement(2555..3499)
                     /locus_tag="VF453_11155"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HEX7688258.1"
                     /translation="MSTIKNRFAEAEPIVLQGRIELRPIAELKPYAANARKHAKSQIT
                     KLAAAIHEFGFVAPVLVGAHGNVLAGHARLEAANRLRMDSVPTLRVEHLTEAQQRAFI
                     IADNRLAELARWDDALLKQELQALLELDFPVEVVGYETPDLDKLLRVIDEDDEAPEVD
                     EDTPVVARLGDLFLLGRHRVYCGDSTLQTSYEAVLGDERARLVFTDPPYNVPIRGHVS
                     SNRKHGEFAMASGEMSLGRNEKRAVRPVFSYFLAERVSPEFELRRTSQHFISCASPLC
                     RLQPFTSHRFLTYHQIARGLMGVTMGSMRKGCRHGSEG"
     gene            complement(3705..4196)
                     /locus_tag="VF453_11160"
     CDS             complement(3705..4196)
                     /locus_tag="VF453_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HEX7688259.1"
                     /translation="MDLHSVLQSIYDSSLSTSIRQVDFLFPGIESLHVIAISFVVGTI
                     AIVDLRLIGYASHRRSANRLIHELLPFTWVAFVAAAITGGLMFASKSIDYAANEPFLW
                     KMCILAVAGLNMAIFHLGIYRRIGEWDETFPVPLPARVAGFLSLALWTAVVFLGRWIG
                     FTT"
     gene            complement(4236..4721)
                     /locus_tag="VF453_11165"
     CDS             complement(4236..4721)
                     /locus_tag="VF453_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HEX7688260.1"
                     /translation="MKDFALWLYQTQLSTAIRNVPSLVAVMQSLHILGVAGVVGSALV
                     SDLRLAGVLATDESPHAVARRYLPWMWGALVLLLLTGLVQIVGEPNRTLTTTTFWLKM
                     GMLLVVFALTLVMRKPLLDPAFDVRAAWWRAHVKPAAWLSLLLWIGVIVCGRWIAYST
                     T"
     gene            complement(4743..6263)
                     /locus_tag="VF453_11170"
     CDS             complement(4743..6263)
                     /locus_tag="VF453_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX7688261.1"
                     /translation="MQCIPAVSFGVSAVAILCWVIPATARADVPPMPTITAVPWSGQL
                     PLSSASRTGTEYVDLAKAGYREQEYYLSGIAPAITANGQSVLQAPYTTRILVRMPADA
                     ARFNGTVIIEPFSWFGERGAGWILTRDYLVRHGYAYVGYTLNINKPERDPKFISDTPE
                     AEAEQIKLYGRIVNFDFMRRFDYARYAPLGTYYDPNKFRRGDGPDPFVPQSQGIAAQL
                     ARLLKSNSPDGPLGSLKVQRIYVDSWAVTAQVWMDYLDQGRHQQWRMPDGRPLIDAYM
                     TGRMSFGEVGGEVLRVPRKLPDDVPFVTVYSQSEAMHDAIEAIPLPPDTDRPRLRYYE
                     VTGMPHLRLADLGTEHTELLAADVGKGDDPRCKTLYDEPAELVVSALLDDMDRWVREG
                     TPMPRETRLTRQGKGLARDTATGNLVGGVRPPWVAVPASQYMTDQETGCGLIYDTKIP
                     YTDVQLRKLYGTYENYRRKFEEATKASVKAGYLLPESASSLQPIATPADFLPARHP"
     gene            complement(6347..7087)
                     /locus_tag="VF453_11175"
     CDS             complement(6347..7087)
                     /locus_tag="VF453_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688262.1"
                     /translation="MRLKANPYLAAALAIAAATVNLPSAEAASGTDISGTWERTPENW
                     YGDNPDAPVLPGGPVKLKEPYATQYRELKESQEKANKAGTPIATTSARCLPEGMPTLM
                     AGLFPLEILQSSRDVVVLGEYLQQVRRIWLGAKMPAPEQIQPSFNGQSVGHWEGDTLV
                     VETRGVRTDTLLYDVPHSKDMKLTERIRLTAPDRLEDQVVIDDPAVLASPYRFTFDYR
                     RSNYKIQEYVCENNEIAVDSEGKAHWKK"
     gene            complement(7151..7636)
                     /locus_tag="VF453_11180"
     CDS             complement(7151..7636)
                     /locus_tag="VF453_11180"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEX7688263.1"
                     /translation="MKSSSITPLSPRRRSVRTGCNLAAGPQARLLTARRLEAVLRRTT
                     YALGVAVIVGLPPLASAHHSFALFDTSKTITVQGVVSRFEFTNPHVEIFVESGDPPKR
                     FKVESGSVNALTRKGGWKPDSIKVGDKVEVTFNPLKTGEQPGGLLVEIKDGNTVLKGG
                     G"
     gene            7822..9258
                     /locus_tag="VF453_11185"
     CDS             7822..9258
                     /locus_tag="VF453_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7688264.1"
                     /translation="MITIRLRSFLAPPRPTSQKRTTVIETELEALNFPDAPKMVTPGI
                     PGPKAAEALALSARTESMARGGGRMPIVMDKAFGVTFKDPDGNTFIDLSAGVGVSSVG
                     RCHPRVVEAMRKQSEALMHAMEVNSTKRTELAAKISEIMPDGLRGDCITFFAQSGSDA
                     IEAAVKFAKRVTGRHQIIAFHGAYHGVWNASGALTTGNAYRKGYGPFMGGVIHAPYPY
                     AYRFPFDTTRKSAEQIAGEYVDYLLNSPYTAADDVAAVIVEPVQGEGGYVPPAPEFLQ
                     ALRKACDRSGALLIVDEVQAGAGRTGKMWAVEHSGVKPDMLTFGKGMGGDVPMAGVVM
                     RSDLAAKIPDGSVPNTFAANSVSAAVALTNIAILQDPALDLLNRAHRLGLEAQERIRS
                     FGSPFVGEVRGRGLMIGIELVEDKETREPLGRDRLGKLMGHLMNNGVLMIPCGRHTNV
                     MRVMPSLTIPRSLMFKALDIFGAGLATL"
     gene            9329..10609
                     /locus_tag="VF453_11190"
     CDS             9329..10609
                     /locus_tag="VF453_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7688265.1"
                     /translation="MTDSLLERRYRVLGRGAPLFYDEPLHLVRGEGVWVYDAQGRRYL
                     DAYNNVPVVGHGHPRVVEAIARQSGTLNTHTRYLHETVVQYADRLTATFAGLVMVMFA
                     CTGSEANDLALRMARRFTGREGIICSNNAYHGNTEAVDELATTFRAGRPGSARVRAIP
                     YPDAYRPHRGLTGIALEDAYVDEVRSRVEEFQRNGLGLAGMLVCPVFANEGLPESPTG
                     FLARAAQVVREAGGLVIFDEVQAGFGRTGHLWGHQPTGARPDIVTLGKPMGNGYPIAG
                     VVARGDIVDAFREEVMYFNTFAGSPVACAAATAVLDVIEEEGLVARAREVGAATTEGL
                     RAMAMRHESIGDVRAKGAFWAVELVRDRVSKAPDAGRAKAVVEHMRRRGVLISRLGQY
                     DNVLKIRPPLVFEAPHAQILLEALEHSMTRVENG"
     gene            10602..11642
                     /locus_tag="VF453_11195"
     CDS             10602..11642
                     /locus_tag="VF453_11195"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7688266.1"
                     /translation="MADLSSPPTSVASLERLALLALDSWGKQAREIQLVKMRENAVFR
                     VVDAQGSVFALRVHREGYHDDDALGAELRWMAALRQDGIEVPTVVPAENGRLFVKARS
                     AECPGERQVDLFEWIDGRALGTSEAGLRGDLGDIFGTYRTVGRLAASLHDHTANWSLP
                     AGFRRHHWDLDGLVGERPVWGRFWDLQALDAEERRILLLARDRVRIELQAFARRPDAD
                     RHYGLIHADLVPENLLLTDRGEVRLIDFDDAGFGWHLFDLATALYFVKDGLGYEAAKE
                     GLIAGYRAHRRLSDADLAALPLFMTARGFTYLGWAHTRPATAESLAILPHVIRLACRE
                     SEDLLSRGRVTP"
     gene            11639..12424
                     /locus_tag="VF453_11200"
     CDS             11639..12424
                     /locus_tag="VF453_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7688267.1"
                     /translation="MSDAGPSAPIRRRPRSRPWLLGTAAALLAGLAVAGIEIRSHEDQ
                     AAVSRADPGRILSDPALRRIALAKGRVVFGNYCASCHGPAGKGDQALGVPDLTDDDHL
                     YGSGTVAETEDIVRYGIRAHNKRGWNLAIMPAYATAVPDPAEPLPPQTPLQVEDLTQY
                     LLSFTGRATDPGAATRGHAAYEAAGCWDCHGHDAMGEPAIGAPNLTDRIWLYGGSHDQ
                     IYRTIARGRAGTSPAFIDTLSPAEIRDVAAYAASLAPMNAESR"
     gene            12421..13338
                     /locus_tag="VF453_11205"
     CDS             12421..13338
                     /locus_tag="VF453_11205"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7688268.1"
                     /translation="MTHSKPATVDDAAATGRADLWDFHRPVARRSLILCAAGALLGLA
                     IAGVGLFTAQGTRTATVPAEDAATVNGVPILRADLVQQVASLYAIDYGRSTPEQRRKV
                     LGDMIREELYVQRGIEIGLPNDDIDVRSALVAATEGQVAQDAMTSKPSERELETWYDG
                     HVADYASEGLMTVHEWLLPTGATDAQPVADRLRRGEPASALGLKSSGRVDDGEEYYFA
                     ARVHLGDRIFDVARMLKDGEVSRPLPSKDGVRILQMVRNVVPVPTPFQEVKSRVLHDV
                     LEDKAARLQEANGRFLRKRADVKYAPDFQ"
     gene            13350..14549
                     /locus_tag="VF453_11210"
     CDS             13350..14549
                     /locus_tag="VF453_11210"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX7688269.1"
                     /translation="MARLLLAIAAALLVAGMPSVASAHTRSESHSSWFVDGGSVHLTF
                     SVSDLEARRVTQDGRPPSDAQLLAYLGPRVGATSAGGTCKLDAAPRPVAAAVGYRRAE
                     FFYACPSAEGIKLHTDAFFELVPTHVNVAQIQTAKGDFVEDILNAESREVDMTEAEGG
                     RLQGAGFLTFVRMGIMHIFTGVDHMSFLLGLVLISRRFRDLAWVISGFTLGHSLTLAL
                     SVTGVIRPYAGFIDALVALTIALIGIENLSVQSRQVGKLALSTALVLFAMAGLKLVGI
                     GLLPVTLLVGAGLFASNYLMVAGHVHDAARLRLVVTLVFGLIHGFGFAADLLSEAIPP
                     AKLAELLVGFNVGVEIGQLTVVSVLTVAVLLLRRARLTFPRAIVVDVCASGLVMIGTY
                     WFVSRSV"
     gene            14635..15885
                     /locus_tag="VF453_11215"
     CDS             14635..15885
                     /locus_tag="VF453_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7688270.1"
                     /translation="MISIPPVDDRAHPATLPGVTERVARKVAIARAQLGPGPWGALSP
                     SDRGRLLSRLADLVDRDAALFGEMTPRDESRSPVRVANDAAHAATILRVAVGWANSLA
                     HRTVTAVPLQGASARSFTALRPIGVVAALMSRHCALPFIARKAAALLAAGCTLVLKPS
                     ERSPHQAIHFSTLCREAGFPDGVVSVVAGRGDVVGRALCEHPDVALVSFAGSPEAGLE
                     VQRNAALRCKPIELDLRGVRVHVVFTDAAIADAVSGCAAELLAGRGSFHTASRRVLIQ
                     RSLSRRFAVELAAAGVEAGPGPVPPQGRPVAGADSDVRARGRDVGPDVAIVEFDTEAE
                     ALALIDEYANVFGVTFWTTSRQRADRLVDACPARRINVNWASTSDMDEPVEDVLRARF
                     GSSAIASPALAYVRETVVTTRSTS"
     gene            15941..16351
                     /locus_tag="VF453_11220"
     CDS             15941..16351
                     /locus_tag="VF453_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX7688271.1"
                     /translation="MAITKFVLQLEDVKRIAAAAEAEARDRQWAVSIAIVDDGGHLLL
                     LHRLDGASALSSHVAPAKAHAAAIGRRETRVHDEAISGGRHALLTLPNVQGLLEGGIP
                     IVHDHQTIGAIGVSGVKSEQDAQIARAGVAALRL"
     gene            complement(16395..18500)
                     /locus_tag="VF453_11225"
     CDS             complement(16395..18500)
                     /locus_tag="VF453_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7688272.1"
                     /translation="MCCGRTRRSGAPGARGPYLVAALALGAGAPAGAQTTAPVDGVQA
                     VVVSGRSANLVGVADSATEGTITAAQLADRPLLRPAEVLESVPGLIITQHSGDGKANQ
                     YFLRGFNLDHGTDFATYVNGMPVNEVTHAHGQGYSDLNFLSPELVDRIDFRKGAYRAE
                     DGDFDTTGIARISYANAVARPSVDLTVGPDQYRRAFAVDSRPLGRCTLLGALDATQTN
                     GPWVQPEGLRKVGGVLRLSSGTIDNGFDITAMGYGAHWTATEEVPQRAIDDGEIGRYG
                     TLAPTDGGKTGRYSLSGEWARADETSATHLSAYVIRYTLDLFSTPSGLQLSQHEQLDQ
                     RKTSGGGIRQVWHLGPKQFDSDLTVGAELRQDRIPRVGLYETADRRRTGTVRQDAVIE
                     TAASTFADLRTQWTPWLRSVLGLRLDGIRAGDTATGGRYNLSNGGKASAGQLSPKVAI
                     AFGPFSRTEFYANWGLGFHSNDARGSISSVDPSDGTAAEKVRLISRAMDLEAGLRTSP
                     LGGWNTGLSLWTTHLASELVFVGDDGVTEPEGGSRRYGVEWWNDATIDRWITVDADMA
                     VSHARFDQVSNGGRYVPNAIPLSVSLGVTADAKGPWLAGVRLRYIGAYPLEETGTERS
                     SPAFTTNVKVGYRAGDRWRVSADILNLFNSKAYDIAYWGSSCTRSEGVACNHGEGYDG
                     RLVHPMEPRTIRLSLRVSL"
     gene            complement(18512..21016)
                     /locus_tag="VF453_11230"
     CDS             complement(18512..21016)
                     /locus_tag="VF453_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7688273.1"
                     /translation="MPSIGDSAVPSAVGTPPEHPSREKRHGAGSLGRFNFKSLMRVCM
                     STSKCQCAMDRSPVPGTQHRLLPLCAALSVMPAAALAQTAPAPAPTAAASAPTAAQAA
                     SAPAAPEVIEVVVTAERRGSTVQKTPISMTAVSGADLKEQGITDVTDLMRDIPGVSAK
                     AGGSGQTEYTIRGLSSAAGVAPTVGFYLDDVPLSSPTTSSGGKSPVDPDLYDLARVEV
                     LRGPQGTLYGASSMGGTIKLVPESPKLNQFGGSAQVIVSGTEGGGLNGTVNGALNVPL
                     VQDTAALRLVVTEKRLSGWIDRVSESTMPIGNADGSRGDVAGQTPDHVTRAANDTRLD
                     GARASMVIQPNDRLSLTPMFLYQRTRLGAPDTFDADPGCCKHYQPFDIREPYKDVFSI
                     GSFKADYDFDAATFTSISAYSKRNKVRVEDETEILQRYFDLPAYSIADGGLGPVTNQE
                     INDTRQFTQEFRLTSNDEGKFRWIVGAFYSRFTSVFAATADGSEQAAAALLGTSDIFH
                     QTIQDVLVQKALFGNATYQFTKDLKLTAGTRIFNSNARDTSVASGILSDGTNHDERAA
                     SKGTTPMVNLSYNVDRTSMLYATAAKGYREGAAQPGVAASCAADLAKLGLTQAPVRFN
                     PDTVWSYEVGSKNRFFDNALTLNAALYTQRWSRVQRYVTLPTCGYLFVDNAGQAEASG
                     FEVEASLRAGAGVTLNMSVGHTHAVYTENDAGSDTVRGQRLDGVAGWTGSGSIQYEHL
                     LADGFRLIGNLGLSYTGRSTINISEPKTLPAYTLADARIGLKFAKWSSSLFIDNITNR
                     HPVTTVVPSLTFNPVGLDQYATTRPRTVGVDVSRDF"
     gene            21330..22175
                     /locus_tag="VF453_11235"
     CDS             21330..22175
                     /locus_tag="VF453_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7688274.1"
                     /translation="MLSVLDLFSSEHTTMTAEQIAESLGLTRTTCYRYTRELGHAGLL
                     VSDGGLFMLGPRIIELDHRIIQSDPLLNAGRPIVCALADSLGATGLLTTCYDDRIINV
                     FEHGATTTRLPVSFGRGTTMPLFRSSTSKAILASMSRSRLKRLWKEHQDEADCQAIGR
                     DWLSFWKALQVIKRQGLCVSTGELDAGMSGISSPILFGTGDVAGCVSLVFPAEDFKVY
                     APELLGARLRAAAGQISRALALAVGEPPGRPVQQEPVVEGRRRRAAGPDTGSSKAGKV
                     AALRS"
     gene            22445..24016
                     /locus_tag="VF453_11240"
     CDS             22445..24016
                     /locus_tag="VF453_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX7688275.1"
                     /translation="MHTFSQQDNSSTGAVTVLEAAVAAGSSVCFANPGTTEMPFVGAL
                     DQVGGMRAVLGLFEGVCTGAADGYGRMSRMPATTLLHLGPGLANGLANLHNARRARTP
                     ILNIVGDHASWHLKYDAPLTSDIESLARPVSGWYRAIASAQHLRRDTVDGLHATLASP
                     GRIATLVVPVDFQQHPAPAQEPPAVVMARPAVIEEERIEAARHVLTSGRRAVLLLGDR
                     ALSERGQLAAGRIAAATGARLFSETFPARTERGGGLPDIDRLPYFPETAIQALEGAVV
                     VLAGAIAPVAYFGYEGLPSELAPAESLHSLAQPGEPADEALERLANALGAPRIVPGTM
                     PAQHIVPAGELTPSAVGRIVANALTEGAIVSVEGGTCGYPFVTASASAARHTILTNTG
                     GAIGQGLPVALGAAVACPDRRVFALQSDGSAQYTIQALWTMARERLPVIVLIASNRRY
                     GILQTELARAGISPGPQASRLTSLDDPPLDWVALAQGYGVPCVRASSGDELRAALCTA
                     LAHTSGPIVIEMRLA"
     gene            24039..24842
                     /locus_tag="VF453_11245"
     CDS             24039..24842
                     /locus_tag="VF453_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7688276.1"
                     /translation="MDLQLKGKAAFITGGSMGIGKAAALELAREGVDVAIAARRLPLL
                     EEAAQDLRRQLGADAGRILPVVVDTTDMDSVTAAVETAASAFGRLDILVNGAAHPGGL
                     VRAELADADPQGLLQDIDIKVVGYLRCAKAAAPHMRRGGYGRIINIGGLTGRGSKQLS
                     GMRNVAIVHMTKTLSDQLGPDGITVNTIHPGVVETPHIHELYEKEARKQGLTAAEVEA
                     NYVKATPIRRVLQPEEMGWLIAFLASPRSGSITGESLGCDGGLTRGIFL"
     gene            24854..25891
                     /locus_tag="VF453_11250"
     CDS             24854..25891
                     /locus_tag="VF453_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7688277.1"
                     /translation="MDFTIMVATAGQGILRSNDDGRTWHRLGLKEPIEFDGVVRALAV
                     DPQRPQRVYAGADCGLCVSDDAGAHWRRAEGAISGMTVWSIAIDPNDPEILYAGTGAP
                     SRSALFKSTDRGETWSRLPAEMPEFCQGVNRPRMLTICVDPDDSRQVWFGVEEGGVWR
                     SRDAGQTFERLDDPYKPIRSSDIHAIAILPATASSPKTTIVLTVNAVYVSDDDGETWD
                     GQLSKQRFDGLYYTRTVLPVPGSERELLLAIGDGTPGFKTRLCRSADRGRTWTETALE
                     TPPNSTFWALGGHLADPGLLYAGTKYGDLFKSRDGGRTWKKEWREFSEITAVAWTPMR
                     APLVAHAQSID"
     gene            25903..26406
                     /locus_tag="VF453_11255"
     CDS             25903..26406
                     /locus_tag="VF453_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7688278.1"
                     /translation="MTAAIEVPPLRPGTQLPVPRVARTHVALYVRDPAASGAWYAEVL
                     GMQEVAREPRWVFMSFGQKHHDIALIKAQTPAELGTIGLQHYGLEIAGGLEELRRLYG
                     MLLRRQVPIVKVTDHKAGIGVYFTDPDGNRLEFFCETAATDEEGKRLMTEYRAASDPV
                     HLEPLFD"
     gene            26419..27024
                     /locus_tag="VF453_11260"
     CDS             26419..27024
                     /locus_tag="VF453_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HEX7688279.1"
                     /translation="MTKAPNFSAFHIRKWFLQVEDTLAGETGQPADGEPLRKIVIAAC
                     IHNPFAGRYVQDLSPWVVPSPDLGREFGRRIREAAQGRAIQSYGKACLVGIDGEYEHG
                     NALLTNPAANPVRDGVGGGKSWVPSTGKRGGPGTVIDIPLAHKDALYVRSHYDTVTAM
                     FADAPNRDEVVLCWAFATRGRLNARLGGLQAGDVKGEDGLV"
     gene            27011..27940
                     /locus_tag="VF453_11265"
     CDS             27011..27940
                     /locus_tag="VF453_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012732966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7688280.1"
                     /translation="MASSDPFAALAGASSTRVQARDGWVESDGLQLRYVEWGRPDAPA
                     IVALHGLRSFAYTWESLAQRLADRFRIIALDQRGRGMSDWDPGRRYYTASYVHDLETL
                     VDELRLQNFVLLGHSMGGAVAFVYSTLHADRLAGLVIEDIGPGSSADSAGADRIKREL
                     RETPNRFASWFEAAAFWRRQRPDVSEEALQARVRHSMKATDDGAIVWRHDAQGIADAR
                     LNATPEQLVALWPHAERVSRPMLVLRGARSDFLSAEVAVEMCRRNPNIRYEEIPAAGH
                     YVHDDNFAAFDAALQRFLGSAPLAAWAGGMANA"
     gene            27933..29573
                     /locus_tag="VF453_11270"
     CDS             27933..29573
                     /locus_tag="VF453_11270"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HEX7688281.1"
                     /translation="MREEGPSRTSVVIVGGGPNGMAAANLLGAYGIRTVVIEREARIL
                     EYPRAVGMDDEALRLFQGVGLADRLVRDMIQNVPMRMFRADGECFAHIRPGTREFGWW
                     RRNIFMQQLAERVLREGASRYDNVELRLGEEVVGLSQDGSGVALQVRDAAGRTYAQLC
                     DYCVAADGGRSPMREMLGLKLVGRTHPIKWVVVDVRNAEVDAPYTALNCDPRRPNVCI
                     YLPYGFRRWEFLVFPHEDAEAIAQPDSVRRLLAPYVSDPDRLEIVRARTYTHHSRVAE
                     RFVEGRVALIGDAAHLSPPWIGQGLNAGLRDAGNLAWKLAGIVRGQLAPSVLQTYEQE
                     RHRHAKAMIDLADAFGAMLMPTSRAKAWLRDRFFSAIRLAPGVRDYVLQMRFKPMPRY
                     EHGVVVPAPGASAVGRMLIQPDVEDATGERRKLDDVLGPWFAVIGWETDPQSGMTDAD
                     RQTWSALGVRFVRISRACSASQPGRRLRSTHGTVCVEDVDNHFADWIAAHPCSIVVVR
                     PDRYVAAQSTARELPEVAQALRSFMPSLQPQPRQREAA"
     gene            29629..29961
                     /locus_tag="VF453_11275"
     CDS             29629..29961
                     /locus_tag="VF453_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX7688282.1"
                     /translation="MTALVEQRIYTLVPGGTAEYIRVYEECGRGVQERILGRMLGYYT
                     REVGELNQLIYLWEFDSMDERASRRERLLADPDFKVFRGRIRHLMVRQENSLLVPAGP
                     RPAQEKKA"
     gene            29958..31397
                     /locus_tag="VF453_11280"
     CDS             29958..31397
                     /locus_tag="VF453_11280"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7688283.1"
                     /translation="MNESYAPPSMYIDGEFIGSSDGVGDPVLNPADGGVLGTVPRGTS
                     ADVARAVEAAQRAFESWRLESPLARSDLLRRVAGLTRERAAQIGLGITLDQGKPLAEA
                     VGEVTASAEHIEWHAEEGRRIYGRVVPARSPAVRQLVLREPIGVCAAFSPWNFPFNQA
                     IRKVVAALASGCTLILKGSPETPSAIVAIARLFHDAGLPPGCLNVVWGSPEEISRQLI
                     ESPIVRMISFTGSVPVGKRLAALAGAHMKRTTMELGGHAPVIVCDDADVDRAADLLAP
                     YKFRNAGQVCVSPTRFLVQRAAFDRFVDRFVDRVGGIRVGRGIDPGVTMGPVANARRL
                     QEMEAFAEDARASGGRIATGGTRLGDQGNFFAPTVVVDPSRTSRLMREEPFGPIAGIL
                     PFDELDDAIRVANSLPFGLAAYAFTRSTERAHAIATRLEAGMVNINHFGMAPAEIPFG
                     GVKDSGFGSEGGVETFDSYLVTKFVTQLN"
     gene            31413..32861
                     /locus_tag="VF453_11285"
     CDS             31413..32861
                     /locus_tag="VF453_11285"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7688284.1"
                     /translation="MSTQQALSMSRCYVDGEWIAATDGSTIAVTNPANGELVGEVPGL
                     GRRETRLAIEAAGRAFIHWRERTAEERATLMRRWAALMMANQEELATLMTREQGKPIA
                     ESRGEIAYAASYVDWFAEEARRIDGEVIPSPWRGKQILAVKEPVGVCAAITPWNFPSA
                     MITRKAAPALAAGCTMVVKPASQTPLSALALARLAELAGIPAGVFNVVTGSASAIGAE
                     LTAHPMVRKLSFTGSTEIGRALAAQCAPTLKKLSLELGGNAPFIVFEDADLDAAVVGA
                     IASKYRNTGQTCVCTNRILVQDAIHDAFVHKLAKAVSELRVGDGLEPDVTQGPLIDER
                     ALAKLDSLVEDARSKGGRVVVGGRRHGLGGTFYEPTLVVEAREDMAIAREEAFGPVAP
                     VFRFRDEAEAVRMANDTEYGLAAYFYSRDLARAWRVAGQLEYGMVGINSGILSTAVAP
                     FGGVKQSGYGREGSRHGIDDYVHLKYLCMGVE"
     gene            33007..34482
                     /locus_tag="VF453_11290"
     CDS             33007..34482
                     /locus_tag="VF453_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX7688285.1"
                     /translation="MALTNQLQDTGRAEAAPGAATGLRRGTLGAGFITLLVISAASPL
                     SVVAGGFPLGIMLGNGAGTPALVVAALVLLLLFAAGYTAMATCVTSAGGFYAMVAQGL
                     GGIAAGAASTVAVLGYVTLQFGLYGLLGAVTSESLGSQLGLAIPWWGCALAAMATVAY
                     FGYHQIDFSARVLAFFVVAEYVVVLTLDCLILGRGGAEGIDIRSFSPHVVSMGNPFIG
                     LLFCFAAFIGFEATTIYSEEAKDPKRSIPVATYAALILVGCFYSFSLWCLVLGAGADR
                     VVGTISALADPTSFLYTLSDRYAGHGLTTLLRGMFVISIYAGLIAFHNSTARYFYSMG
                     REGLLPAALGRTHPKYRSPHVGSMLQTVLCAVVVVAFAVAKADPVLTLFAWLSNLATV
                     CVLVLMIATAVSVIRFFHVDPRGHGRMRTMVLPGLGAVGLCMVLVLAVLNFHVLTGAS
                     RGISHGVVAFVPVAIVVGAWMAARLRRRDPARYLRLGQNRP"
     gene            complement(34574..35536)
                     /locus_tag="VF453_11295"
     CDS             complement(34574..35536)
                     /locus_tag="VF453_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7688286.1"
                     /translation="MSLNIDEFEDIHQHASSVREWQQIYSQLTAGPLRSSLCQIRNAR
                     FHAFRERINQRVVQHGEAPRGRVCFAVPLLVPGSVRMQGREADDASIFVLRGGEEFMF
                     HMPMGMDMLAITFDEALFDNAVSSTGCVDEINTLLKQPVVRVPIERLAASRRRLMAMF
                     SEAAAESALPEPPNGVEMNLEQAMLDELLGLIADPACDRTQQHGSSVRSFIVEKCHRM
                     TISDKVNAPSVVELCERLRISRRTMQNSFRSVAETTPVNYIRSIRLNGVRRELMSTRA
                     AELSIGDAAARWGFFHLSHFAAEYHELFGELPSQTRRGDRAADR"
     gene            35834..37285
                     /locus_tag="VF453_11300"
     CDS             35834..37285
                     /locus_tag="VF453_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7688287.1"
                     /translation="MTKHLKHFVAGESVDSSDGARMSLVSPIDESEYATAARGTVEDV
                     RRAVAAARAQLDGGAWSKLSGLQRGLLLSRLADLVERDTGLLADMDANAIGRSPLEPR
                     MMDVPNAVANLRAAAGWANQLEGRTIPTSGYMGTPTLSYTVREPIGVVGAIVPWNSPL
                     MITTWKLAALLAAGCTVVVKPSEETPQSALHLAALCKEAGFPDGVVNVVTGYGDVVGR
                     ALCEHPDVAKISFTGSPEAGRAIQRIVGPMFKRVALELGGKSPQIVFDDASFDDAVRG
                     CTLGLFVNQGQVCAAGSRILVQRGIAQRFAAALAEAASVVKVGDPRQPGVQMGPVAKK
                     AQYERVNRYIQIGIDEGAELLAGGVAQSERGWFVKPTIFANASNTMTIAREEIFGPVG
                     TVIAFDTEDEAIALANDSDYGLAATVWTTSLARAHRVAGAVKAGAVGVNCWSPLDANL
                     PWGGLKTSGIGREGGLAGAIAYTEEKVVTVLLP"
     gene            37320..37958
                     /locus_tag="VF453_11305"
     CDS             37320..37958
                     /locus_tag="VF453_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX7688288.1"
                     /translation="MRWRNTLEAYGSVSMLFHWVIAAAFVSAYVVVYYVIWFVDPETS
                     VKPALFGTTPDPTRVVPILNIHWILGVTIGVLALPRLLWRLAGPVPRHVPGSRVEHLA
                     ADAAHWALYGLLILMPVSGYMTTYDPTNFGLFVIPPCRATAFAAWLRSTFSLSTKELE
                     DLAWSVHSFLGQWIAWPLVVLHVAAALVHHFVRKDGVLRRMLPVACDASRSP"
     gene            complement(38037..40133)
                     /locus_tag="VF453_11310"
     CDS             complement(38037..40133)
                     /locus_tag="VF453_11310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7688289.1"
                     /translation="MSEARGYSHQAAQARFLFAFTASHWFDPLEETVIHARRAREELL
                     QGGDLQNACFTYLSSTSALFDSAPLLEDSLEEIDAAIALAERTANQHAGDSYVLQRQL
                     AHKLRGRAPEDTASDAPFVDLPCFNEGGRNPFATALQHVTCGLAAALFGDDAALLHHA
                     VAAFQHEECTECFYTSAQSKLLYALALTRRLDMVPHEHPGICAQLDSLQGWWEERAAD
                     APANFGHLALLIKAERARAQGDFHAAARLYDVAMREVAPRHRPWHHAFIAERAGLLHL
                     EQGLLHTGQRLLNEARTHYATWGALGKVRHMERVHGFLGSHGVAGRSLESRRSATSVI
                     MTSDAIDLLGVLRASQALSSETSLARLTVRVTELLGAMTGATGVSVAVWDDQLHEWCL
                     LSPAPDSAMSQSIPIAQAAANGRLPLTAFRYADRIREPLLVDDAVRDPRFATDPYFAG
                     QQQCSLLVVPIDSQSVPRAMVILENRLSSSAFSAARLDAVLLIAGQLAVSLDNALVYA
                     SLERKVAERTDALEEANRNLALLAVTDPLTGISNRRHFAEVLQAEWLRAMRPGAPIGL
                     AMIDIDQFKLYNDHYGHQAGDACLRRVAATLKGGLRMTADLVARYGGEEFVLVLPSTD
                     IAGTESACNRLRSEIASLRLPHEKSDHGVVTASIGITAIVPREGDNPEHHLALADAAL
                     YEAKRAGRNRVARSPS"
     gene            complement(40054..41280)
                     /locus_tag="VF453_11315"
     CDS             complement(40054..41280)
                     /locus_tag="VF453_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688290.1"
                     /translation="MINALRRDGILQCGDDGWRWDANEIRRYIGHGTVLDLIFARIGK
                     LPPETCALLETLACLGNSIEMSLLRAATSLGADALVSMLREPLEEGLLTPMLGTDART
                     TIGWRFRHDRVQQAAYSRLEPQQRGRAQLTLARRLANHPEFCLQAAEQYLLSADAVRD
                     ADERRQVARLFQHAAATARKAVSYAAVGRWLTEAKALLEGDDRADELLAWEIDVDLHA
                     ALYALGRLEDADRLFRHIAARDASPLELADAACVQISSLCNRNRLEEALALGIQMLQR
                     LGAHVDEAPKSAAVTGFRELPPHADDVGRPEMTDPKAVASAKIINRLLPAALTMDSMD
                     WMVRESRAMWIESGPCAELVGPLCHTGFVTVFHGDYRKGVRRRSPRPGSERGKGLFPS
                     SRAGPVPVRLHRQSLV"
     gene            complement(41210..42961)
                     /locus_tag="VF453_11320"
     CDS             complement(41210..42961)
                     /locus_tag="VF453_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7688291.1"
                     /translation="MSASALSVTALDLSTAPACEDVLYHSERTRIVRRRLKRDGTWVV
                     CKELLGPEAGHRLRHEQAMLARMSSVAGVAHLVGAQVETRTLVLEDVSGISLAQALGG
                     QSLTIQHKIEFAVALARIVADVHSQGIVHRDINPANILVSGPDWRPTLIDFNLASSLA
                     EERPTFTHESAITGTLPYLAPEQTGRTSRSVDQRADLYSLGATLYEVVAGRPPFEGTD
                     PLQLIRDHLARMPYRPSQLNPAVPDMLSDILMRLLAKEPDHRYQSADGLVRDLTRLSN
                     SHAPFALGERDFPTHLTAPSRLIGRDAEILALSDAVDASVRTARCAVLVSGSPGVGKS
                     ALVGELRTIVTTRGGWFVIGKFEQYRQEAAWTAFRSAMRELGRLLLAEPPAELERQRQ
                     RILTSLGSNAGILTDALPEFLTLLGPQADRGEVSPVEAEARLIQAVIDLLGAVASTAR
                     PIVIVIEDLQWAHPTSLRLIERLISDADIRGLLVVATYRDAEIDAAHPLAQLLVRWHG
                     SPVAPHPLSLGNLPPRVRRTRLRDAAPSLPGGHAAVGSAGNADRGQPLRHCRIDQRLA
                     PRRHSAVRRRRLAVGCE"
     gene            43370..43597
                     /locus_tag="VF453_11325"
     CDS             43370..43597
                     /locus_tag="VF453_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688292.1"
                     /translation="MTHSHHRHAMPSLILRCAWLRAAPTPPISSGSRIPEAAGRPRAF
                     VPPWIATFKPWQADTRLARQWVRAEFVWQLN"
     gene            43614..45161
                     /locus_tag="VF453_11330"
     CDS             43614..45161
                     /locus_tag="VF453_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7688293.1"
                     /translation="MRLTHLKIGVRLALAFGAVLCLLSMLATVAVMRTDGLREDVKAL
                     TAGIAAESALADRMRLAATRQAVRVRNVLMVSEPEEMAAEEQLLKADTHDYDAAKGSL
                     ARLIGRDVDPRKRAILARIDAAEEARDLSGQVVKMALENHKFPARGLLGEKANPAQAK
                     WMAAVDEMVALNEKLSAEAVAGVEQACAKTRDIVLAISLAALFVAAVIGHAVTRSITV
                     PISQAVDVARRVAARDLSVRVDSDVPDETGQLLRALGAMTESLLTVVCSVRASSDNLA
                     GAAIEIASGNADLSNRTERQAASIQQTASSMEELSGTVRTSAANSTHARQLSTEAADR
                     AREGGDAVGKVVRTMGDIVSSSRRIADIIGVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVATEVRTLAQRSATAAKEIKQLIAESVDKVDEGTRLVDVAAGTIEQVVCAVQRV
                     AGIVSEITSATVEQSAGIDLVNDAVAQMDHATQQNAALVEQVAAAADSMRSQVQRLVG
                     DVSVFRV"
     gene            45777..48032
                     /locus_tag="VF453_11335"
     CDS             45777..48032
                     /locus_tag="VF453_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688294.1"
                     /translation="MLPKPLRATSLACLAALAACGGGGGSPAAPAPDTHAITITGQPV
                     AATPAADGSQTWSVTASSDIALSYQWAIDGRAVAGATSPSFTMTPALSDDGEQVSVTV
                     SNSATSVVSAAVTVSVAAVTGLNVFAGRIGGAGYVDGAASVARLGHVEQGWSASALGT
                     DGAGDLLVVDPSNFVVRRVTPAGVVSTVAGVAGHWGSTDGPVASALFPSASGVVADAA
                     GNVYVSTYREIRKITPAGVSTLAGSESTSREVDGTGAAAGFDAVLGLAIDPAGNLYAA
                     DFAASIRKITPAGVVTSYYDASKHPGQRSFQSMVFGADGNLYAVDAMSDALVKISPAG
                     VITNVAGGIVDNPDGSVSVGPFGGQSDLAIGADGVLTSFNLGSATTPPTMTRVAADGT
                     TSTVALDLPDNDRVGGAVAFGPGFALLDQTQGAVYLVDTSGKATLFVGQPPRSGNVDG
                     RGGAATFDRPGKVVVDAAGNAYVLETDGYATNHTVVRKVATDGTVSTVFASEDWYGAI
                     AVDAGGQLYGTQYDEVDKLNPDNTATVVVDRSQLAAAGGSNPFPALRDLALAPDGHLL
                     ACTDAGLFSVAVAARTVAALNAVANCGAVAATASGRIYELQTVYATTAAADSVLLLDA
                     TTPGTPTLVAGGTVGYADGTGAAAAFGDFQLGADATGDTVRGRVALSADEDGNLYMVD
                     PDNHAVRMITPAGVVTTLAGGPTTISQVVTGATAGLDYPGGVAPLPGTGGRQLFITDT
                     YEPSLLVLRRP"
BASE COUNT         7545 a        16239 c        16650 g         7978 t
ORIGIN      
        1 cgcccgtatg ctcgcgccct gcagcttcat gcccgcgatc agcacgcgct cttcaggcct
       61 gagttgcctg tacttgcttc cttcttccat cgcagcacct taccggaggt gaggtgttgc
      121 acttcagact tgaggtcgcc cctctttatc aggtgggttg tggtcaggcg ggctgggtgt
      181 tggaagcacc cggcttcgcc gtttgcctcg gaatgccgcc gaggcgccgg aagaagtgca
      241 cgcagctcag tggcggaatg tgggcgcgct tgtctcgaag cccatttcgg ccggggtcaa
      301 tagctcgaag tgctcgggtg tcgctgagca gtcgcaccct gcgacgtcgg tgaacaccgc
      361 cgcgaagccg gacactacct gcgacggcgc cgtccgaagg gactcgatgg cgcgcgccaa
      421 tgcgtcgcac tgagcgacgc caggccgttg ttcggcttcg atgctgcatc ggtcgctacc
      481 aactgcgaac agttctcgta gcacgaccaa agcgaggttg cggccggcaa ggaacatcga
      541 ggccggtgga atcgaggaga catcggtgat gtcgtggggc atggaagggc atcctttcga
      601 ggtggcaatg agcgcgcacc gcggcgagtg ctgcacgcgc gggaacatcg tcggcggtgc
      661 cgtcgctagg cgagtcgact gacgaattcg atggctgttg aatcgctctt ggcgaggttg
      721 cacggaacgc aggccgcaac tagattgacc agctcgtctg agccatcaag cgcctttggg
      781 agcatgtgct cgacgtgcca tttcccctcg agggacaagc cagcgccgca gtagtggcaa
      841 cgcccggcgg acttgccgaa gacctcccgc cgccgcctgg aaacagcacg cggccgactg
      901 ccagccccgc ccaacggagg aagcacctcg gtcggccaga gtgcggcctg ctcgcccacc
      961 tgacgagtcg tggactccgc caaagcgtcc gccgcgcgca gtagcttccg ggcggaggcg
     1021 agggcatcaa gcttgtcctc tgagacgctg aggacggcgc ctgcgctcgg gtcgatgacc
     1081 atgtagcgga cagcgtgcaa cccacgcgat atgcagggtt gcgtggctag ggcgtagcgc
     1141 cccttgccga agacgccgcc gaatatgggg cgatgtcgta gagctgacgc gctcatgagc
     1201 gcacccgcgc attgggtaca gtgtcggtag ccatgttgca cagtccttcc acggtgtgtg
     1261 ctgtatggtc agacgggccg ggccttgccg ggctcggttc cgtcgccttg cctgagtccg
     1321 tcaggctgcg gtttttcggc gctccttggg gttcatcgga acaacgacgc ccttttgctc
     1381 gaccttgccg cagaactttg cccagtcggt catcatgcga acgcgcttct ggaagaggtc
     1441 gccgcggcga tacgcagcct caaccttgtc gccgatggtg tgtgccaggg ccatttcggc
     1501 tacatggttt gggtagttcg tccgctccgc cgcccaatcc cggaatgtcg accgaaagcc
     1561 gtgcggaacg acgggcacat cgagccgccg aaccacggat agcaaagtca tgtccgacag
     1621 cgtgccggct ccacggccag cgaagacata gggagagtct gtccgctttg gaagcgactt
     1681 gagcaaggtc acggccgtcg gtgacagggg cacacgatgc ggcttccgcc ccttcattcg
     1741 ttccgccggg acgttccaga cagcctcgtc aaagtcaaac tcggcccaga ctgcaccacg
     1801 tacctcgcca gaccgcgcgg cggtcagtat cgcgaactcg agagctctgg cccccatgcc
     1861 ctccgactga cgaagtcgga gcatgaaacc gcccatttcg tcgatgggca gcgcaggatg
     1921 atggtccttg cctcgaacct cgttcgccgc cggcagcact ttggcaaggt ggcccttcca
     1981 gcgcgccgga ttcaagccgt cgcggtagcc acaggcggtt gcccaatcaa gtactgcttc
     2041 gattcggccc cgaagtctgc tggcagtctc cgtcttcgtt cgccagatag gctctaacac
     2101 cgccagcacg tgagacatcc cgacgtcgcg aactagcaga ttccccatga ccggaccggc
     2161 gtatgtctcg agcgtattgc gccactgttg accgctcttg gcgttcttcc acttggccgc
     2221 gttggccttg atgtacttcg cggcgcattc gtcgaagctc agtgctgacg cccgtgcggc
     2281 gcgtagagcg ctcttgctcg cgcgggcctc ctcgatgggg tcgacgcctt gcctgacttt
     2341 caagcgtgca gctcgagcgg cttcgcgagc gccagcaagc gtgacggctg gataaccgcc
     2401 caatcccata tcccggcgct tcccgccgat ggtgacgcgc aaaacccagg tgcggccgcc
     2461 cgtctccagc acctgtagcg ccaggccggg cacctcgccc acgaagtaca gcccgggcgc
     2521 tgaaaggcgc gacaccgcca aggcggtcaa ctctttagcc ttccgagcca tgcctgcagc
     2581 cctttcgcat cgaacccatc gtgacaccca tcaatccccg tgcgatttga tgatacgtca
     2641 agaagcgatg cgaagtgaag ggctgcagcc tacatagggg ggaggcgcac gaaatgaagt
     2701 gttgtgaggt tcggcgaagt tcgaattctg gggacaccct ctccgccagg aagtacgaaa
     2761 acacgggcct cacggcccgt ttttcatttc tacccaacga catctcgccc gacgccatcg
     2821 cgaactcgcc gtgcttgcgg ttgctgctga cgtggccgcg gatcggcacg ttgtagggcg
     2881 ggtcggtgaa gaccaggcgc gcgcgttcgt cgcccagcac ggcctcgtag gacgtctgca
     2941 gggtgctgtc gccgcagtag acgcggtgcc gaccgagcag gaagaggtcg ccgagccgcg
     3001 cgacgacggg ggtgtcctcg tcgacttcgg gcgcttcgtc atcctcgtcg atgacgcgca
     3061 gcagcttgtc gagatcgggc gtctcgtagc cgacgacctc cacggggaag tcgagctcga
     3121 gcagggcctg cagctcctgc ttcagcagcg cgtcgtccca gcgggcgagt tcggccagcc
     3181 ggttgtcggc gatgatgaag gcgcgctgct gcgcctcggt gagatgctcg acgcgcaacg
     3241 tgggcacgct gtccatccgc aggcggttgg ccgcttccag acgggcgtgg ccggccagca
     3301 cgttgccgtg cgcaccaacg agaacgggtg cgacgaagcc gaactcgtgg atggcggcag
     3361 ccagcttcgt gatctgcgac ttggcgtgct tgcgcgcgtt ggccgcgtag ggcttgagct
     3421 cagcgatggg gcggagctcg atgcgtcctt gcaggacgat gggctccgcc tccgcgaagc
     3481 ggttcttgat ggtcgacatg acgtctccag tggccgaaga cccgtcgacc gcacgggcgc
     3541 acgacgtggt gcttcaggtg gcctgaaaca ggaaagttgc ggggtcgggc atcacgccca
     3601 agctgcgcac ctcgttgacg tcacgtcgcc gttgaatcta ccgcgaacga ttccaggaat
     3661 ggtcgatgca acgacgactg aacgaccgct cgtcgacgct cgcctcaggt ggtgaagccg
     3721 atccagcgac ccaggaacac gacggcggtc cacagcgcca gcgacagaaa gccggcgacg
     3781 cgggcgggaa gcgggacggg gaacgtctcg tcccattcac cgatccgccg gtagatgccg
     3841 agatgaaaga tggccatgtt cagtcccgcg accgccagga tgcacatctt ccacaggaat
     3901 ggctcgttcg ccgcgtagtc gatcgacttg gatgcaaaca tgaggccgcc cgtgatcgcc
     3961 gcggcgacga aggcgaccca cgtgaacggg agcagttcgt ggatcagtcg attcgcgctg
     4021 cggcggtgcg acgcgtaccc gatgaggcgg aggtcgacga tggcgatcgt gccgacgacg
     4081 aagctgatcg cgatcacgtg cagggactcg atgccgggga agagaaagtc gacctgacgt
     4141 atcgaggtcg acagcgacga gtcataaatg gactgaagga cggagtggag atccacggcg
     4201 catttacctg gatggttgtt gaagacggcc tggaatcacg tcgtcgaata ggcgatccat
     4261 cgtccgcaaa cgatcacgcc gatccagagc agcagcgaca gccatgcggc gggcttcacg
     4321 tgtgccctcc accaggctgc acggacgtcg aatgcagggt cgagcagcgg cttgcgcatg
     4381 acgagcgtga gcgcgaagac gacgagcagc attcccatct tgagccagaa ggtcgtggtc
     4441 gtcagtgtgc gattcggctc gccgacgatc tggaccagcc ccgtcaggag cagcaggacg
     4501 agcgcgcccc acatccaggg caggtagcgg cgcgccacgg cgtgcggcga ttcgtccgtc
     4561 gccagcacgc ctgcaagccg caggtcggaa acgagggccg atcccaccac gcccgccacc
     4621 cccaggatgt gcaggctctg catgacggcc accagcgatg gcacattccg tatcgccgtg
     4681 ctcagctggg tttggtaaag ccagagggcg aagtctttca acggttcgac tcccaacatc
     4741 gttcaggggt ggcgcgccgg caggaagtct gccggcgtcg caatcggctg gaggctcgag
     4801 gcactctccg gaagaaggta ccccgccttg acggaggcct tcgtggcttc ttcgaacttg
     4861 cggcggtagt tctcgtacgt cccatacagt ttgcgcagct gaacgtccgt gtacgggatc
     4921 ttggtgtcgt agatcagtcc gcagcccgtc tcctggtcgg tcatgtactg gctggccggc
     4981 acggcgaccc acggcggacg aacgccgccg accaggttgc ccgtcgcggt atcgcgcgcc
     5041 aggcccttgc cctggcgcgt gaggcgcgtc tcccgcggca tgggcgtgcc ttcccgcacc
     5101 cagcgatcca tgtcgtccag cagggcagag accaccagct cggcgggctc gtcgtacaag
     5161 gtcttgcagc ggggatcgtc gcccttgccg acgtctgcgg cgaggagctc ggtgtgctcg
     5221 gtacccaggt ccgccaagcg caggtgaggc atgcccgtca cttcgtagta gcgcagccgc
     5281 gggcgatcgg tgtcgggcgg caagggaatc gcttcgatgg cgtcgtgcat ggcctccgat
     5341 tggctgtaca ccgtgacgaa tggcacgtca tcggggagct tgcgcgggac ccggagtact
     5401 tcgccgccga cctcgccaaa ggacatcctg ccggtcatgt atgcgtcgat cagcggccga
     5461 ccgtcgggca tgcgccattg ctggtggcga ccctggtcca ggtagtccat ccacacctgg
     5521 gcggtcacgg cccagctgtc gacgtagatc cgctgcacct tcaacgatcc cagcgggccg
     5581 tccggcgaat tcgacttcag cagccgtgcg agttgcgcgg cgatgccttg ggactgcgga
     5641 acgaagggat cgggtccgtc gccgcggcga aacttgttgg ggtcgtagta cgtgcccaat
     5701 ggcgcatatc gcgcgtagtc gaaccggcgc atgaagtcga agttgacgat cctgccgtaa
     5761 agcttgatct gttcagcctc ggcctccggc gtgtccgaaa tgaacttggg atcgcgctcc
     5821 ggcttgttga tgttgagggt atagccgaca tacgcatacc cgtgccggac gagatagtcg
     5881 cgcgtcagga tccatcctgc gccgcgctcg ccgaaccagc tgaagggctc gatgatcacc
     5941 gttccgttga agcgggccgc atcggcgggc atgcgcacca ggatccgggt cgtgtagggc
     6001 gcctgcagaa cgctttgccc gttcgccgtg atggcggggg cgattccgga caggtagtac
     6061 tcctgctcgc ggtaacccgc cttcgcgaga tcgacgtact ccgtgccagt gcggctggcc
     6121 gacgacagcg gaagctggcc cgaccagggc accgccgtga tcgtcggcat cggcgggacg
     6181 tctgcgcgcg cggtcgccgg gatcacccag cagaggatgg ccacggccga gacgccgaac
     6241 gatacggccg ggatgcattg cattcgagaa ctccaggtca tctcacgatc tcgcaaactt
     6301 cctcgaggcc gccgacggcg ggcgaggcca ggcgcgctac ccggtactac ttcttccagt
     6361 gcgccttgcc ttcgctgtcg accgcgatct cgttgttctc gcagacgtat tcctggatct
     6421 tgtagttcga cctgcggtag tcgaacgtga agcgataggg cgatgcgagc acggcgggat
     6481 cgtcgatcac cacctggtcc tcgagccggt cgggtgccgt cagccggatg cgctcggtga
     6541 gcttcatgtc cttgctgtgc ggaacgtcgt acagcaaggt gtcggtgcgc acgccgcgcg
     6601 tctcgacgac cagcgtgtcg ccttcccaat gcccgacgga ctggccgttg aaagaaggct
     6661 ggatctgctc cggcgcgggc atcttcgcgc cgagccagat gcggcggacc tgctgaaggt
     6721 attcgccgag cacgaccacg tctctcgagc tctgcagtat ctcgagggga aacaagcccg
     6781 ccatgagcgt cggcatgcct tcgggcaggc atcgggcgct cgtggtggcg atcggggtgc
     6841 cggccttatt ggccttctcc tgactctcct tgagctcgcg gtattgcgtg gcgtagggct
     6901 ccttcagctt gacggggccg cccggcagga ccggagcgtc tgggttgtcg ccataccagt
     6961 tctccggcgt cctctcccag gttccgctga tgtccgtccc tgatgccgcc tcggcggacg
     7021 gaaggttcac agtcgccgcc gcgatcgcga gggcagcggc aagatacgga tttgccttca
     7081 gtctcatgag aaaaatctcc agatgatcgg gcctggcgac gccagcgcct cccctcggcg
     7141 cgaggtgcaa ctagcctccg cctttgagga cggtgttgcc atccttgatc tccacgagca
     7201 ggccgccggg ctgctccccg gtcttcaggg gattgaaggt gacttcgacc ttgtcgccga
     7261 ccttgatcga gtctggcttc cagccgccct tgcgcgtcaa ggcattgacg cttcccgatt
     7321 ccaccttgaa gcgcttgggc ggatcgccgg attcgacgaa gatctcgacg tggggattcg
     7381 tgaattcgaa ccggctgacg acaccctgga cagtgatggt cttgctcgtg tcgaacaagg
     7441 cgaacgaatg gtgcgcactc gccagcggcg gcaggccgac gatcacggca acgccgagcg
     7501 cataggtcgt gcggcgaagt acagcctcga ggcgccgagc cgtcaatagg cgggcttgcg
     7561 gaccggcggc gaggttgcat cctgtacgaa ccgagcgccg acgcggcgac agcggagtaa
     7621 tcgatgacga tttcaaggta gggccctcca gtgggcgctg ctgtactgat tcgagtctag
     7681 gacagggcgg gaggccgcgc gtagcccgaa tcggcattcg tgctcggcag tctggatgtg
     7741 cgaaggccgc aagacccgcg gccctcatgc gcgccttggc tcgccgcgat tgccgattcg
     7801 ggctaaccgc gcgacggcag gttgatcacc attcggctgc gctcattcct cgcacctcct
     7861 cgacccactt cgcaaaagag aaccaccgtg atcgaaactg aactggaagc cctcaatttc
     7921 cccgacgccc cgaaaatggt caccccgggc attcccggcc cgaaggctgc cgaggcattg
     7981 gcgttgtccg ctcggacgga gtcgatggcc cggggcgggg ggcgcatgcc catcgtcatg
     8041 gacaaggcct tcggcgtcac gttcaaggat ccggacggca atacgttcat cgacctgtcg
     8101 gctggtgtcg gcgtgagcag cgtcgggcgc tgccatccca gggtggtcga ggccatgcgc
     8161 aagcaatcgg aggctttgat gcacgccatg gaggtcaaca gtaccaagcg cacggagttg
     8221 gccgccaaga tttccgagat catgccggac gggctgcggg gcgactgcat cacgttcttc
     8281 gcgcagagcg gcagcgacgc gatcgaggcg gcggtcaagt tcgcgaagcg ggtcaccggg
     8341 cggcaccaga tcatcgcgtt ccacggcgcc taccacggcg tgtggaacgc ctcgggcgcg
     8401 ctgaccaccg ggaacgccta ccgcaagggg tacggcccgt tcatgggcgg cgtgattcat
     8461 gccccgtacc cgtatgccta ccggttcccg ttcgacacga cgcgcaagag cgcggagcag
     8521 atcgccggcg agtatgtcga ctatctcctc aattcgccct acacggccgc cgacgacgtg
     8581 gcggcggtca tcgtcgagcc cgttcagggc gagggcgggt acgtgccgcc ggcgcccgag
     8641 ttcctgcagg cgctgcgcaa ggcatgcgac cgcagcggcg cgctgctgat cgtggacgag
     8701 gtgcaggccg gcgcggggcg gacgggcaag atgtgggcgg tcgagcattc cggcgtcaag
     8761 ccggacatgc tcacgttcgg caagggcatg ggcggcgacg tcccgatggc gggcgtggtc
     8821 atgcgatccg atctcgcggc caagattccc gacggctcgg tgccgaacac cttcgcggcg
     8881 aactccgtct ccgcggccgt cgcgctgacc aacatcgcga tcctgcagga tcccgcgctg
     8941 gacctcctga accgcgccca ccgactcggc ctcgaggccc aggagcgcat ccgcagcttc
     9001 ggcagcccgt tcgtcggcga ggtgcgcgga cgcggactga tgatcggtat cgagctcgtc
     9061 gaagacaagg agacccggga gccgctcggc cgcgatcgcc tcggcaagct catgggccat
     9121 ctgatgaaca acggcgtgct catgattccc tgcggccgcc ataccaacgt gatgcgcgtg
     9181 atgccgtcgt tgaccattcc gcgctcgctg atgttcaagg cgctggacat cttcggcgcc
     9241 ggcctggcga ccctctgact gagcgatggc ctcgtcctcg cggtaccttc gccggtggcg
     9301 cggggccgct tcaggagcag cgcaggaaat gacggattcg ttgctggagc gtcgctaccg
     9361 cgtgctgggc aggggcgcgc cgctcttcta cgatgagccg ctgcacctgg tccgcggcga
     9421 aggcgtctgg gtctacgacg cccaggggcg acgctacctc gacgcctaca acaacgtccc
     9481 ggtggtcgga cacggccacc cgcgtgtggt ggaggccatt gccaggcagt cgggcacgct
     9541 caacacccat acccggtatc tgcacgagac cgtggtgcag tacgcggacc gcctgacggc
     9601 caccttcgcg ggactggtca tggtcatgtt cgcatgcacc ggaagcgagg ccaacgatct
     9661 ggcgctgcgg atggcgcgca ggttcaccgg ccgcgagggc atcatctgct cgaacaacgc
     9721 ctaccacggc aacaccgagg cagtggacga attggcgacg acgttccggg cgggccggcc
     9781 cggcagcgcg cgcgtgcgcg cgattcccta tccggacgcc tatcgaccgc atcgcggctt
     9841 gaccggcatc gcgctggagg acgcctacgt ggacgaagtc cggtcgcgcg tcgaggagtt
     9901 ccagcgcaac ggcctgggtc tggcggggat gctcgtctgc ccggtcttcg ccaacgaggg
     9961 gctgcccgaa tcgccgaccg gattcctggc tcgcgcggcg caggtcgtcc gggaggccgg
    10021 cggtctggtg attttcgacg aagtccaggc gggattcggc cgtacaggac atctctgggg
    10081 gcatcagccg accggtgcgc gtccggacat cgtcacgctc ggcaagccga tgggcaacgg
    10141 ctacccgatc gccggggtcg tcgcgcgtgg cgacatcgtc gacgcgttcc gcgaggaggt
    10201 gatgtatttc aacaccttcg cgggcagtcc cgtcgcctgc gcggcggcga cggccgtgct
    10261 ggacgtgatc gaggaggagg gcctcgtcgc gcgcgcccgc gaagtcgggg ctgcgacgac
    10321 ggagggcctg cgggccatgg caatgaggca cgagtcgatc ggtgatgtgc gcgcgaaggg
    10381 cgccttctgg gctgtcgaat tggtgcggga tcgcgtgtcc aaggccccgg acgccggccg
    10441 cgccaaggcg gtcgtcgagc acatgcgcag gcggggggtg ctgatcagcc ggctgggaca
    10501 gtacgacaat gtgctcaaga tccgtccacc gctcgtcttc gaagcgcctc acgcgcagat
    10561 cctgctcgaa gccctggagc acagcatgac ccgggtcgaa aatggctgat ctttcaagcc
    10621 ccccgacgtc cgtcgcctcg ctcgagcgcc tcgccctgct ggcattggat tcctggggaa
    10681 agcaggcccg cgagatccag ttggtcaaga tgcgcgagaa cgccgtcttt cgcgtcgtcg
    10741 atgcgcaggg cagcgtcttc gctctgcgcg tccatcgcga gggctatcac gacgacgatg
    10801 cccttggcgc cgagctgcgg tggatggccg cgctgcggca ggacggcatc gaggtcccga
    10861 ccgtcgttcc ggcggagaat ggccggctct tcgtcaaggc gcgcagcgcg gagtgccccg
    10921 gtgagcgcca ggtcgatctg ttcgagtgga tcgacggccg cgcactcggc acttccgagg
    10981 cgggcctgcg gggcgatctg ggggatatct tcggaaccta ccgcacggtc ggacgcctgg
    11041 ccgcatcctt gcacgaccac acggcgaact ggtcgctgcc cgcgggcttt cgccggcacc
    11101 attgggacct cgacgggctg gtaggcgagc ggccggtctg gggccggttc tgggatctgc
    11161 aggcgttgga tgccgaggag cgcaggatcc tgctgctcgc ccgcgaccgc gtgcgcatcg
    11221 aattgcaggc attcgccagg cggcccgatg ccgatcgcca ctatggcctc attcacgccg
    11281 acctcgtgcc cgagaacctg ctgctcaccg accggggcga ggtgaggctg atcgacttcg
    11341 acgacgcagg attcggctgg cacctgttcg acctggcgac ggcgctctac ttcgtcaagg
    11401 atggcttggg gtacgaggcg gccaaggaag gcctgatcgc cggctatcgc gcgcatcgcc
    11461 gactgtccga cgccgatctg gccgcgctgc cgttgttcat gacggcgcgc ggcttcacct
    11521 acctcgggtg ggcgcacacg cggcccgcta cggcggagtc gcttgccatc ttgccgcacg
    11581 tgatccgcct ggcttgccgg gagtcggaag acctgctgag ccgggggcgc gtcacgccat
    11641 gagcgacgcc ggcccgtccg cgccgatccg caggcggccc cggtcgaggc cctggctcct
    11701 cggcacggcg gccgctcttc tggcaggtct tgcggtggcc gggatcgaga tccgttcgca
    11761 cgaagaccag gcggcggtgt cgcgggccga tccgggacga atcttgtccg atccagcgtt
    11821 gcgccggata gcgctcgcga aaggacgggt ggtgttcggc aattactgcg cctcgtgcca
    11881 cggcccggcc gggaagggcg accaggcgct gggcgtaccc gacttgacgg acgacgatca
    11941 tctctatggt tcgggcaccg tggcagagac cgaagacatc gtgcgctacg gcatccgcgc
    12001 ccacaacaag cgcggctgga acctggcgat catgccggcc tacgccaccg cggtacccga
    12061 ccctgcggag ccgctgccgc cgcaaacccc gttgcaggtg gaagacctga cgcagtacct
    12121 gctttcgttc accggacgag cgaccgaccc tggcgcggcg acgcgagggc acgccgccta
    12181 cgaggcggca ggatgctggg actgccatgg ccatgacgcg atgggcgagc cggccatcgg
    12241 agcgcccaac ctgaccgacc ggatctggct ctatggcggc agccatgacc agatctaccg
    12301 gacgatcgcg cgcgggcggg ccggcacgag ccccgccttc atcgacacgc tctcgccggc
    12361 ggagatccgt gatgtcgcgg cctatgcggc ctcgctcgcc cccatgaacg cggagtccag
    12421 atgacccatt cgaagcccgc caccgtcgac gacgccgccg ccacaggccg ggccgacctg
    12481 tgggacttcc accgacccgt tgcccgccgc tcgctgatcc tgtgcgccgc cggtgccctg
    12541 cttgggctgg cgatcgcggg cgtcggcctg ttcacggcgc agggcacccg aactgcgacc
    12601 gtccccgccg aggacgcggc caccgtcaac ggcgtgccca tcctgcgcgc ggatctcgtc
    12661 cagcaggtcg cgtccctcta tgccatcgac tatggccgat ccacgccgga acaacgccgc
    12721 aaggtgctcg gcgacatgat ccgcgaggaa ctgtacgtcc agcggggcat cgagatcggc
    12781 ctgcccaacg acgacatcga cgtgcgcagc gcgctcgtcg ccgctaccga agggcaggtg
    12841 gcacaggacg ccatgacctc gaaaccctcg gaacgggaac tcgagacctg gtacgacggc
    12901 cacgtcgccg actatgcgag cgaaggcctg atgacggtgc acgagtggct gctgccgacc
    12961 ggggcgaccg acgcccaacc ggtggccgat cgcttgcggc ggggcgagcc ggcatccgcc
    13021 ctgggcctga aatcctccgg ccgcgtcgac gatggcgagg aatactactt tgctgcgcgc
    13081 gtgcacctgg gagaccgcat ctttgatgtc gcccgcatgc tcaaggatgg cgaggtctcg
    13141 cgtcccttgc cgtccaagga tggtgttcgc atcctgcaga tggtcagaaa cgtggtgccg
    13201 gtgccgacgc ccttccagga agtgaagagt cgcgtcctgc acgatgtcct cgaggacaag
    13261 gccgcgcgcc tccaggaagc gaacggacgc ttcctgcgaa agcgtgccga cgtcaagtac
    13321 gcgccggatt tccagtgaag accgtggcca tggcaaggtt gctcctcgcg atcgccgccg
    13381 cgttgctggt ggccggcatg ccatcggtgg cctcggcgca tacccggtcg gagtcgcact
    13441 cgagctggtt cgtggatggt ggctcggttc acctgacgtt ctcggtctcg gatctcgagg
    13501 cgcggcgtgt cactcaggac ggacggccgc cctcagacgc gcaactgctc gcctacctcg
    13561 gcccgcgcgt cggtgccact tccgcgggcg ggacgtgcaa gctcgacgcc gcgcccaggc
    13621 cggtagcggc ggcggtgggg taccgccgcg ccgagttttt ctacgcctgc ccctccgccg
    13681 aaggcatcaa gctgcatacg gacgcgttct tcgagctggt gcctacgcac gtcaacgtgg
    13741 cgcagatcca gaccgccaag ggcgacttcg tcgaggacat cctgaacgcg gagtcgcgcg
    13801 aggtcgacat gacggaggca gaaggcggcc gcctgcaagg cgccggcttc ctgaccttcg
    13861 tgcgcatggg catcatgcac atcttcacgg gcgtcgatca catgagcttc ctgctggggc
    13921 tcgtgctgat ctcgaggcgc ttcagggatc tcgcatgggt catcagtggc ttcaccctgg
    13981 gccacagtct cacgctggcg ctctcggtga cgggcgtcat ccgtccctac gccgggttca
    14041 tcgacgcgct cgtggcgctc accatcgcgc tgatcgggat cgagaacctg tcggtgcagt
    14101 cgcggcaggt cggcaagctg gcgctctcga cggcgctggt gctcttcgcg atggccggac
    14161 tcaagctcgt gggaataggc ctcctgccgg tgacgctgct tgtcggcgcc ggactgttcg
    14221 cgtcgaacta cctgatggtc gccggccacg tccacgacgc cgcgcggctg cgcctggtcg
    14281 tgacgctggt gttcgggctc atccatggct tcggcttcgc ggccgacctg ttgagcgagg
    14341 ccatcccccc ggccaagctc gccgagctgc tggtgggatt caacgtcggc gtcgagatcg
    14401 gccagctgac ggtggtgtcg gtgctgaccg tcgccgtgct gttgctgcgc cgcgcgaggc
    14461 tgacgtttcc acgcgccatc gtcgtcgacg tctgcgcgtc gggtctcgtc atgatcggga
    14521 cgtattggtt cgtatcgcgc agcgtctagc tcgctggcgg gccaggcgtg agcacgctcg
    14581 ctgggaagta cggtcggacc gttgatttcg gatatttccc ttgttgttgc cgccatgatt
    14641 tccattcccc ccgtcgacga ccgcgcgcac cctgccaccc tgccaggggt gaccgagcgc
    14701 gtcgcccgca aggtggccat cgcacgcgcc cagctcggcc ccggcccgtg gggtgcgctg
    14761 tcgccgagcg atcgaggccg gctgctgagc cgcctcgcag acctcgtcga ccgggatgca
    14821 gcgctgttcg gggagatgac gccgcgggac gagtcgcgct cgcctgtgcg ggtggcgaac
    14881 gacgcggcgc acgccgccac gatcttgcgt gtcgccgtgg gatgggccaa ttcgctggcg
    14941 catcgcaccg tcacggccgt gccgctccag ggtgcgagcg cacggtcgtt cacggcgctt
    15001 cggccgatcg gggtcgtggc cgccctcatg tcgcgccatt gcgcgctacc gttcatcgct
    15061 cgtaaagcgg ccgcgttgct cgcggccggc tgcacgttgg tgctcaagcc ttcggaacgg
    15121 tcaccgcatc aggctatcca tttctcgacg ctgtgccggg aagccggctt cccggacggc
    15181 gtcgtcagtg tcgtcgccgg gcgtggcgac gtcgtggggc gggcgctctg cgagcatccc
    15241 gacgtcgcgc tcgtcagctt cgccggcagc ccggaggccg ggctcgaggt ccagcgcaac
    15301 gccgcactcc ggtgcaagcc gatcgagctc gacttgcggg gggtgcgcgt ccacgtcgtt
    15361 ttcacggacg ccgcgatcgc agacgcggtg tccggctgcg ccgccgagct gctggcaggg
    15421 cgggggtcgt ttcataccgc aagcaggcgc gtcttgatcc agcgaagctt gtcccgccgc
    15481 ttcgccgtgg aactggccgc ggcaggcgtg gaagcgggac cgggccccgt tccgcctcaa
    15541 ggccgtcctg tcgcaggagc ggactccgac gtgcgcgcgc gcggacggga cgtcggtccc
    15601 gatgtggcca tcgtcgaatt cgacaccgag gcggaagccc tggcgctgat cgacgaatac
    15661 gcgaacgtgt tcggcgtgac gttctggacg accagccgtc aacgcgccga tcgcctggtc
    15721 gacgcctgcc cggctcgcag gatcaacgtg aactgggcga gtacttccga catggacgag
    15781 ccggtcgaag acgtcctgcg tgccaggttc ggttcgtccg cgatcgcgtc acccgcgctc
    15841 gcctacgttc gagagaccgt cgtgacgacg cgttcgacgt cctgacgacc atcacgacgc
    15901 tgctgccgcg acgagatcat ccacccaagc actgcaagac atggccatca ccaaattcgt
    15961 tcttcagctc gaggatgtca agcggatcgc cgccgccgcg gaagccgagg cacgcgaccg
    16021 gcaatgggcc gtgagcatcg ccatcgtcga cgacggcgga catctcctgt tgctgcaccg
    16081 gctagacggc gcatccgccc tgtcgtccca cgtggcgccg gccaaggcgc atgccgcggc
    16141 catcggacgg cgcgagaccc gggtccatga cgaggccatc agtggcgggc gtcatgccct
    16201 gctgacgttg ccgaacgtcc agggactgct ggaaggaggc atcccgatcg tgcacgacca
    16261 ccagacgatc ggtgcgatcg gcgtcagtgg cgtcaagtcc gagcaagacg cgcagatcgc
    16321 cagggcgggc gtcgcggccc tgcggctctg aggcgcggcg cccgcgccac gcgtcggcac
    16381 ggcggcggct gccgctacag gctcactcgc agcgacagcc ggatcgtccg gggctccatc
    16441 ggatggacca ggcgtccgtc gtagccctcg ccgtggttgc aggcgacgcc ttcgctgcgc
    16501 gtgcaggagg agccccaata ggcgatgtcg tacgccttgc tgttgaacag gttcaggatg
    16561 tccgcgctga cccgccagcg gtcgccggcg cggtatccca ccttgacgtt cgtggtgaat
    16621 gccggcgacg atctctccgt gccggtctcc tccagcggat aggcgccgat gtatcgcagg
    16681 cgcacgccgg ccagccacgg gcccttggcg tccgccgtca cgcccagaga taccgacagc
    16741 ggtatcgcgt tgggcacgta acgaccgccg ttggagacct ggtcgaaacg cgcgtgcgag
    16801 accgccatgt ccgcgtcgac ggtgatccag cggtcgatcg tcgcgtcgtt ccaccactcg
    16861 acgccatagc ggcgagatcc gccttccggt tccgtgacgc cgtcgtcgcc gacgaacacg
    16921 agttccgatg cgaggtgcgt cgtccacagg gacaggcccg tgttccagcc gcccagcggc
    16981 gacgtgcgca ggcccgcttc caagtccatg gcgcgcgaga tcaggcggac cttttccgcg
    17041 gcagtgccgt cggagggatc gacggacgag atcgatcccc gcgcatcgtt gctgtggaag
    17101 cccaggcccc aattggcata gaactccgtg cgcgagaagg gaccgaaggc gatggccacc
    17161 ttggggctca gctggcctgc gctggccttg ccgccattgg acaggttgta gcgtcccccg
    17221 gtcgcggtgt cgcctgcccg gatgccgtcg agccgcaggc cgaggacgct gcgcagccac
    17281 ggtgtccatt gcgtgcgcaa gtccgcgaaa gtgcttgccg ccgtctcgat caccgcgtcc
    17341 tggcggaccg tgccggtgcg ccggcgatcg gcggtctcgt acaggccgac ccgggggatg
    17401 cgatcctggc gcagctccgc gccgaccgtg aggtcgctgt cgaattgctt cgggccgaga
    17461 tgccagacct gcctgatgcc accgcccgat gttttgcgct ggtcgagttg ctcgtgctgg
    17521 ctcagctgca ggccggacgg cgtggagaac aggtccagcg tataccggat gacgtaggcg
    17581 ctgaggtgcg tcgcgctcgt ctcgtcggcg cgagcccatt cgcccgacag gctgtagcgc
    17641 ccggtcttgc cgccgtcggt cggtgcgagc gtgccgtagc ggccgatctc gccgtcgtcg
    17701 atcgcgcgtt gaggcacttc ttccgtggcg gtccagtgcg cgccgtagcc catggcggtg
    17761 atgtcgaacc cgttgtcgat ggtgcccgac gacagccgca gcacgccgcc gaccttgcgc
    17821 agtccctcgg gctgcaccca ggggccgttg gtctgcgtgg cgtcgagggc tcccagcagc
    17881 gtgcatcgcc cgagcggccg gctgtcgacc gcgaacgcgc gccggtactg gtccggaccg
    17941 acggtcaggt cgaccgaggg cctggccacc gcgttcgcgt aggagatgcg tgcgatgccc
    18001 gtcgtgtcga agtcgccgtc ctcggcgcga tacgctccct tgcgaaaatc gatccggtcg
    18061 acgagctccg gcgacaggaa gttcaggtcc gagtagccct ggccgtgcgc gtgggtgacc
    18121 tcgttgaccg gcatgccgtt gacgtaggtc gcgaagtcgg tgccatggtc gaggttgaag
    18181 ccacgcagga agtactggtt cgctttcccg tccccggagt gctgcgtgat gatcaggccg
    18241 ggcaccgact cgagcacctc ggccggtcgc aggagcgggc ggtccgcgag ctgcgccgcg
    18301 gtgatcgtgc cctcggtggc cgagtcggcg acgcccacca ggttggcgga gcggcccgag
    18361 acgacgacgg cctgcacccc gtccaccggc gccgtcgtct gggcgcccgc cggcgcgccg
    18421 gcaccgaggg cgagcgccgc gacgaggtag gggcctcgag cgccgggcgc gccggatcgc
    18481 cgcgttcggc cgcagcacat gcaggcgggc gtcagaagtc gcgggacacg tcgacgccga
    18541 ccgtgcgcgg cctggtcgtc gcgtattggt cgagcccgac ggggttgaac gtgagcgacg
    18601 gaacgaccgt ggtgacgggg tgtcggttgg tgatgttgtc gatgaagagg gagctgctcc
    18661 acttggcgaa cttgagcccg atgcgcgcgt ccgccagcgt ataggccggc agcgtcttgg
    18721 gctcggagat gttgatcgtc gaccgcccgg tgtagctcag gcccaggttg ccgatcaggc
    18781 ggaagccgtc ggccagcagg tgctcgtact ggatcgatcc ggatccggtc catcccgcca
    18841 cgccgtccag tcgctgaccc cggaccgtgt cgctgccggc atcgttctcc gtgtagaccg
    18901 cgtgcgtgtg gcccacggac atgttcagcg tcacgccggc gccggcgcgc agcgatgctt
    18961 cgacctcgaa gcccgaggct tctgcctgcc ccgcgttgtc gacgaagaga tagccgcagg
    19021 tgggcaaggt gacgtagcgc tgaacgcggg accagcgctg ggtgtagagc gccgcgttca
    19081 acgtgagcgc gttgtcgaag aagcggttct tgcttcccac ttcgtagctc cagacggtgt
    19141 ccggattgaa tcgcaccggc gcctgggtca ggccgagctt ggcgaggtcc gcggcgcagc
    19201 tggccgcgac gccgggctgc gcggcccctt cccggtagcc cttggcggcc gtcgcgtaca
    19261 gcatggaggt gcgatccacg ttgtaggaca ggttgaccat cggcgtggtg cccttcgacg
    19321 cagcgcgctc gtcgtgattc gtcccgtccg agaggatgcc ggacgcgacg gacgtgtcgc
    19381 gtgcgttgga attgaagatg cgcgtgccgg ccgtgagctt caggtccttg gtgaactggt
    19441 aggtggcgtt gccgaagagc gccttctgca ccaggacgtc ctggatggtc tggtggaaga
    19501 tgtcgctcgt gcccagcagc gccgccgcgg cctgctccga gccgtcggcc gtcgcggcga
    19561 agaccgacgt gaaccggctg tagaaggcgc cgacgatcca gcggaacttc ccttcgtcgt
    19621 tcgaggtcag gcggaactcc tgcgtgaact gccgcgtgtc gttgatttcc tgattcgtca
    19681 cggggcccag gccgccgtcg gcgatgctgt aggccgggag gtcgaagtag cgctgcagga
    19741 tctcggtctc gtcctcgacg cgaaccttgt tgcgcttgga gtaggcggaa atggacgtga
    19801 aggtcgcggc atcgaagtcg tagtcggcct tgaagctgcc gatcgagaac acgtccttgt
    19861 agggttccct gatgtcgaac ggttgatagt gcttgcagca cccagggtcg gcatcgaacg
    19921 tgtcgggcgc gcccagccgc gtgcgctggt acaggaacat tggcgtgagg ctgagccggt
    19981 cgttcggctg gatcaccatg gaggcgcgcg caccgtcgag ccgggtgtcg ttggcggcgc
    20041 gggtcacgtg gtcgggcgtc tgccctgcga catcgccgcg ggagccgtcg gcgttgccga
    20101 tgggcatcgt gctttccgag acccggtcga tccagccgct cagccgcttt tcggtgacca
    20161 ccagccgcag cgcggcggtg tcctgcacca gcgggacgtt cagggcgccg ttgaccgtgc
    20221 cgttcaggcc gccaccctcc gttccggaca cgatgacctg ggcactcccg ccgaactgat
    20281 tcagcttcgg cgattcgggc acgagcttga tggtgccgcc catggagctg gcgccgtaca
    20341 gggtgccttg cgggccgcgc aggacttcca cgcgtgccag gtcgtagagg tccgggtcga
    20401 cgggggactt gccgcccgag gacgtcgtcg gcgaggacag cggaacatcg tcgaggtaga
    20461 agcccaccgt cggcgccacg cccgcggcgc tcgacaggcc gcggatcgtg tattcggtct
    20521 ggccggagcc gcccgccttc gcgctcacgc cgggaatgtc gcgcatcaga tccgtgacgt
    20581 cggtgatgcc ctgttccttg aggtctgcac cggagacggc ggtcatgctg atcggcgtct
    20641 tctgcacggt ggagccacgc cgctccgccg tgacgaccac ctcgatcacc tccggggccg
    20701 ccggcgccga tgccgcctgg gcggcagtcg gcgcgctggc cgccgccgtc ggcgcgggtg
    20761 ccggcgcggt ctgggccagt gcagcggcgg gcatcacgct caaggccgcg cagaggggga
    20821 gcagccgatg ctgcgttccc ggaacggggg accggtccat tgcgcattga cacttcgatg
    20881 tagacatgca gaccctcatc agagatttga aattgaatcg accgaggctt cccgccccat
    20941 gtcgcttctc gcgggatgga tgctccggcg gtgttccgac cgccgacggg acggcgctgt
    21001 cgccgatcga cggcatgagt ctcggcgttg atgccaaccc gcggttagcc caaatcggca
    21061 gccgagcagg ctgcgattcc tgccgcacgt cgagcccgat ggcgtccacg gccgccgata
    21121 gaaatttcca taatgcggca ttcgcccaga aaatggcgga tttcgcgccg gtcgaccgat
    21181 tggctcgata ggcgcactgc ctttcgttgc cgacgtttga aagcggccag gcgaccgcgc
    21241 cgcggtcgag tcgtgtcagc cacccatgac atgacctaag atccggcgtc catcacgtcg
    21301 cgagtccacc agtgtccagc ctcacgcgca tgctgtccgt cctcgacttg ttctcgagcg
    21361 agcacacgac gatgaccgcg gaacagatcg cggagtcgct cggcctgacg cggacgacgt
    21421 gctatcgcta tacccgcgag ctcggccacg ccgggctgct ggtgagcgac ggcgggctct
    21481 tcatgctggg accccgcatc atcgagctcg accaccgcat catccagtcg gatccgctgc
    21541 tgaacgccgg gcgtccgatc gtttgtgccc tcgcggattc gctgggcgcg acggggctgc
    21601 tgacgacctg ctacgacgat cgcatcatca acgtgttcga gcatggagcg acgaccacgc
    21661 gtcttccggt ctccttcggg cgcggtacga caatgccgct gttccgttcg tccacctcca
    21721 aggcgatcct cgcgtcgatg agccgcagcc ggctcaagcg tctttggaag gagcatcagg
    21781 acgaggcgga ctgccaggcg atcgggcggg attggctgtc cttctggaag gcattgcagg
    21841 tcatcaagcg ccagggcctg tgcgtgtcga ccggcgaact cgacgcaggc atgtcgggta
    21901 tctcctcgcc gatcctgttc ggcaccggcg acgtcgcggg ctgcgtgtcg ctcgtgttcc
    21961 cggccgagga cttcaaggtc tacgcgccgg aattgctcgg cgcccgcttg cgcgccgcgg
    22021 cggggcagat ctcgcgcgca ttggcgctgg cagtcggcga accgcccggt cgcccagtcc
    22081 agcaggaacc cgtcgtcgaa ggccgccgcc gacgtgccgc cggtccggac accggatcgt
    22141 ccaaggcagg caaggtcgcc gcgctgcgct cctgacctgc tcgtgcacgc cgtcgggatg
    22201 acggcacctc atgcccgcgg ccgcgcgcgt gacgcgcggc gaagcagcgg caggcttccg
    22261 cacagctcca cggcaattcc tccccattca tctcgctggc cgaactggcc gcgatgggcg
    22321 ccgccgcatc tccttggtcg cgccgggccg gatccggggg gacttcggcg tgccgatttc
    22381 ggtcattgag atgttgtaaa tgccgtattt tgggattacg atcatttcca catcaggacg
    22441 ggacatgcat actttttccc aacaggacaa ctcctccacc ggggccgtga cggtcctcga
    22501 ggccgccgtc gccgcgggtt cgagcgtctg cttcgcaaac ccgggaacga ccgagatgcc
    22561 gttcgtcggg gcgctggacc aggtcggcgg catgcgcgcc gtgctgggac tcttcgaggg
    22621 cgtctgcacc ggcgctgccg acggttacgg gcgcatgtcg cgcatgcccg ccaccacatt
    22681 gctgcatctc gggccggggc tcgccaacgg gcttgccaac ctgcacaacg cacggcgcgc
    22741 gcgcacgccg atcctcaaca tcgtcggcga ccatgccagc tggcacctga agtacgacgc
    22801 gccgctcacg tcggacatcg aatcgctggc gcgtcccgtg tccggctggt atcgggccat
    22861 cgcgtcggcg cagcacctgc ggcgcgacac ggtcgacggt ctgcacgcga cgctggcatc
    22921 gccggggcgg atcgccacgc tggtggtgcc ggtcgatttc cagcagcatc cggcgcccgc
    22981 gcaggagccg cccgcggtcg tgatggccag gcccgccgtg atcgaggagg aacgcatcga
    23041 ggcggcccgc cacgtgctga ccagcggccg tcgcgcggtg ctgctcctgg gcgatcgtgc
    23101 cctcagcgag cgcgggcaac tcgcggccgg ccgcattgcc gccgccaccg gcgcgcgcct
    23161 gttcagcgag accttccccg ctcgcaccga gcgcggcggc gggttgcccg acatcgaccg
    23221 gctgccgtac tttccggaga cggccatcca ggcactggag ggtgcagtgg tcgtgctcgc
    23281 gggtgcgatc gcccccgtcg cctacttcgg ctacgagggc ctgccgagcg aactggcgcc
    23341 ggccgaatcg ctgcattcgc tcgcgcagcc cggcgagccc gcggacgagg cgctcgaacg
    23401 cctggccaac gctctcggcg cgccccggat cgtgcccggg acgatgccgg cgcagcacat
    23461 cgtgcccgcg ggcgagctca cgccgtcggc cgttggccgg atcgtggcga atgcgctgac
    23521 ggaaggggcc atcgtctcgg tcgagggggg cacctgcggg taccccttcg tcacggcctc
    23581 cgcctcggcg gcgcgccata ccatcctgac caacacggga ggcgcgatcg ggcaggggct
    23641 gccggtggcc ctgggcgcgg ccgtggcatg cccggatcgc cgcgtcttcg cgctccagtc
    23701 ggacggcagc gcgcagtaca cgatccaggc gctgtggacg atggcgcgcg agcggctgcc
    23761 ggtcatcgtc ctgatcgcct cgaatcgtcg ctacgggatc ctgcagacgg agctcgcgcg
    23821 cgccggcatc tcgccgggcc cgcaggcctc gaggctgacc tcgctggacg atccgccgct
    23881 ggactgggtc gcgctcgccc agggctacgg cgtgccgtgc gtccgcgcgt cgagcggcga
    23941 cgaactccgg gccgcgctct gcacagcgct ggcgcacacc tcgggcccga tcgtgatcga
    24001 gatgcgcctg gcctgatcac cccttctttc gaggaactat ggatctgcaa ctcaagggca
    24061 aggccgcctt catcaccggc ggcagcatgg gcatcggcaa ggccgcggcg ctggagctcg
    24121 cgcgcgaggg cgtcgacgtc gcgatcgccg cgcgccggct gccgctgctg gaagaggccg
    24181 cgcaggatct tcgccggcag ctcggcgcgg atgccggacg catccttccc gtcgtggtgg
    24241 acaccaccga catggactct gtgaccgccg cggtggagac ggcggcgtcc gcgttcggcc
    24301 ggctcgacat cctcgtcaac ggcgcggcgc atcccggcgg cctcgtgcgc gcggaactgg
    24361 ccgatgccga tcctcagggc ctcctgcagg acatcgacat caaggtcgtc ggctacctgc
    24421 ggtgcgcgaa ggccgccgcg ccgcacatgc gccgcggcgg ctacggccgg atcatcaaca
    24481 tcggcgggct gactggccgc ggcagcaagc agctctcggg catgcgcaac gtcgccatcg
    24541 tgcacatgac caagacgctg tcggatcagc tgggtcccga cggcatcacc gtcaatacga
    24601 tccaccccgg cgtggtcgag acgccgcaca tccacgagct ctacgagaag gaggcccgca
    24661 agcagggcct gaccgcggcg gaggtcgagg cgaactacgt caaggcgacg ccgatccgtc
    24721 gcgtgttgca gcccgaggag atgggctggc tcatcgcctt cctcgcctcg ccgaggtccg
    24781 gatcgatcac cggcgaatcc ctcggctgcg acggtggcct gacccgcggc atcttcctct
    24841 gacggagctt cccatggact tcacgatcat ggtcgccact gcgggccaag gcatcctgcg
    24901 cagcaacgac gacggcagga cctggcatcg cctcggcctc aaggaaccga tcgaattcga
    24961 cggcgtcgtc cgtgcgctgg cagtcgatcc gcagcggccg caacgcgtgt acgccggcgc
    25021 cgactgcggc ctgtgcgtca gcgacgatgc aggcgcgcac tggcgccgcg cggagggtgc
    25081 gatctcgggc atgacggtct ggtcgatcgc catcgacccg aacgacccgg aaatcctcta
    25141 tgccggcacc ggcgcgccgt cgcgctcggc gctgttcaag tcgaccgatc gcggcgagac
    25201 ctggtcgcgc ctgcctgccg agatgcccga gttctgccag ggcgtgaatc gcccgcgcat
    25261 gctgacgatc tgcgtcgatc ccgacgacag ccggcaggtc tggttcgggg tcgaggaggg
    25321 cggcgtctgg cgcagccggg atgcggggca gaccttcgag cgcctggacg atccgtacaa
    25381 gcccatccgc agctccgaca tccatgcgat cgcgatcctg ccggcgacgg cgtcgtcgcc
    25441 caagacgacg atcgtgctga cggtcaatgc ggtgtacgtc agcgacgatg acggcgagac
    25501 ctgggacgga cagctctcga agcagcgctt cgacgggctg tactacaccc gcacggtgct
    25561 gcccgtcccg gggtcggagc gcgaactgct gctcgcgatc ggcgatggca cgccgggctt
    25621 caagacgcgc ctgtgccgtt ccgccgaccg cggccgtacc tggaccgaga cagcgctgga
    25681 gacgccgccg aactcgacgt tctgggccct cggaggccac ctggccgacc ccgggcttct
    25741 ctacgccggc accaagtacg gcgacctgtt caagtcgcgg gatggcggcc gcacctggaa
    25801 gaaggagtgg cgggagttca gcgagatcac cgccgtggcc tggacgccga tgcgcgcacc
    25861 gctggtcgcg cacgcgcaat cgatcgattg agggagtccg acatgaccgc tgccatcgag
    25921 gtgccgccgc tgcgtcccgg cacgcagttg cccgtccccc gcgtcgcgcg cacgcacgtg
    25981 gccttgtacg tccgcgaccc ggcggcctcc ggcgcgtggt acgccgaggt gctcggcatg
    26041 caggaggtcg cccgcgagcc gcgctgggtc ttcatgtcgt tcgggcagaa gcaccacgac
    26101 atcgcgctga tcaaggcgca gacgccggcc gagctcggga cgatcggcct ccagcactac
    26161 ggcctggaga tcgccggcgg cctcgaggag ctgcgacgcc tgtacgggat gctgctgcga
    26221 cgtcaggtgc cgatcgtgaa ggtcaccgat cacaaggccg gcatcggcgt ctatttcacg
    26281 gaccccgacg gcaaccggct cgagttcttc tgcgagaccg ccgccaccga cgaggagggc
    26341 aagcggctca tgaccgagta tcgcgcggcg agcgatcccg tgcacctgga accgctcttc
    26401 gactgatcca tccacgccat gacgaaagct ccgaacttct ccgcattcca catccgaaag
    26461 tggttcctgc aggtcgagga caccctcgcg ggcgaaaccg gccagccggc cgacggcgag
    26521 ccgctgcgca agatcgtgat cgcggcgtgc atccacaacc cgttcgcggg tcgctacgtc
    26581 caggacctgt cgccctgggt cgtgccgtcc cccgacctgg gccgcgagtt cggccgccgc
    26641 atccgcgagg cggcgcaggg gcgcgcgatc cagagctacg gcaaggcctg cctggtcggc
    26701 atcgacggcg agtacgagca cggcaacgcg ctgctcacca atccggcggc caatccggtg
    26761 cgagacggcg tcggcggggg caagtcgtgg gtgccctcga ccggcaagcg cggcggcccc
    26821 ggcaccgtga tcgacatccc gctggcgcac aaggacgccc tgtacgtccg ttcgcactac
    26881 gacacggtga cggcgatgtt cgcggacgcg ccgaaccgcg acgaggtcgt cctgtgctgg
    26941 gccttcgcca cgcgcgggcg cctcaacgcc cgcctgggcg gcctgcaagc cggcgacgtc
    27001 aagggcgaag atggcctcgt ctgatccctt cgcagcgctg gcgggcgcat catcgacccg
    27061 agtacaggca cgtgacgggt gggtggagag cgatggcctg cagctccgct atgtcgaatg
    27121 ggggcgaccc gatgcgcctg ccatcgtcgc cctgcacggc ttgcgcagtt tcgcctacac
    27181 gtgggagtcg ctcgcccagc gcctggctga ccgattccgc atcattgctc tggaccagcg
    27241 tggtcgcggc atgagcgact gggatcccgg ccggcgctac tacacggcgt cgtacgtgca
    27301 cgacctggag acgctcgtcg acgagctccg gcttcagaac ttcgtgttgc tcgggcattc
    27361 gatgggcggc gcagtcgcat tcgtctactc cacgctccac gccgaccgcc tggccgggct
    27421 cgtgatcgaa gacatcggtc caggatcgtc ggccgactca gccggggccg accgcatcaa
    27481 gcgcgaactg cgcgagacac cgaaccgctt cgcctcgtgg ttcgaggcgg ccgccttctg
    27541 gcgacgccag cgtcccgacg tttccgagga ggcattgcag gcgcgcgtgc ggcattcgat
    27601 gaaggccacc gatgacggcg ccatcgtctg gcgccacgac gcacaaggca tcgccgacgc
    27661 gcggttgaac gccacgccgg agcagctggt cgccctctgg ccccacgccg aacgcgtctc
    27721 gcggccgatg ctcgtcctgc gcggcgcgcg ctccgacttc ctctccgccg aggtcgcggt
    27781 cgagatgtgc cggcgcaacc cgaacatccg atacgaggag attccggccg ccgggcacta
    27841 cgtgcacgac gacaacttcg ctgcgttcga cgccgcgctg cagcggttcc tgggcagcgc
    27901 gccgctggcg gcgtgggccg ggggcatggc gaatgcgtga agagggtccg tcgcggacgt
    27961 ccgtcgtcat cgtcggcggc ggccccaacg gcatggccgc cgccaacctg ctgggcgcgt
    28021 acggcatccg tacggtcgtc atcgagcgcg aggcgcgcat cctcgagtat ccgcgcgccg
    28081 tgggcatgga cgacgaggcg ctgcggctgt tccagggcgt gggactcgcc gatcgcctgg
    28141 tgcgcgacat gatccagaac gtgccgatgc gcatgttccg cgcggacggc gagtgctttg
    28201 cccacatccg gcccggcacg cgcgaattcg gctggtggcg gcgcaacatc ttcatgcagc
    28261 agctggcgga gagggtgctg cgcgagggtg catcccgcta cgacaacgtc gagttgcggc
    28321 tgggcgagga agtggtcggc ctttcccagg atggatccgg cgtcgcactg caggtgcgcg
    28381 atgccgcggg ccggacctat gcgcagctgt gcgactactg cgtggcggcg gacggcggcc
    28441 gcagtccgat gcgcgagatg ctcggattga agctggtcgg ccggacgcat cccatcaagt
    28501 gggtcgtggt cgacgtcagg aacgccgaag tcgacgcgcc ctacacggcg ctcaattgcg
    28561 acccgaggcg cccgaacgtg tgcatctacc tgccgtacgg attccggcgc tgggagtttc
    28621 tcgtcttccc gcacgaggat gcggaggcca tcgcgcagcc ggattccgtg cgtcggctgc
    28681 tggcgcccta cgtgtcggat ccggatcggc tggagatcgt ccgcgcgcgc acctacacgc
    28741 accactcgcg cgtcgcggag cgcttcgtcg agggccgcgt cgccctgatc ggtgatgcgg
    28801 cgcacctctc gccgccttgg atcggacaag gcctcaacgc gggactgcgg gatgccggca
    28861 acctcgcctg gaagcttgca ggcatcgtgc gcgggcagct ggcaccgtcg gtgctccaga
    28921 cctacgagca ggaacgccat cgccacgcga aggcgatgat cgacctcgcc gacgccttcg
    28981 gcgcgatgct gatgccgacc agccgtgcca aggcctggct gcgcgatcgc ttcttctcgg
    29041 caatccgcct ggctccgggc gtgcgtgact acgtgctgca gatgcgcttc aagccgatgc
    29101 cgcgctacga gcacggtgtc gtggtgccgg caccaggggc gagcgccgtg ggccggatgc
    29161 tgatccagcc cgacgtcgag gacgcgaccg gcgaacggcg caagctcgac gacgtactcg
    29221 ggccgtggtt cgccgtcatt ggctgggaga ctgatccgca gtccggcatg accgacgcgg
    29281 atcgccagac gtggagcgcg ctgggcgtgc gtttcgtccg catcagccgg gcgtgcagcg
    29341 ccagccagcc aggacggcgc ctgcgatcga cgcacggcac cgtgtgcgtc gaagacgtcg
    29401 acaaccactt cgcggactgg atcgccgcgc atccatgctc catcgtggtc gtgcgcccgg
    29461 accgctatgt cgcggcgcag tccacggctc gggagctgcc cgaggtggcg caggcattgc
    29521 gctcgttcat gccatccctg caaccgcagc cgcggcagag ggaggcagcg tgagtgcccc
    29581 gcgtgccgca gccgcgcatg cgacaacctt ttccgcggag atgaccgcat gactgccttg
    29641 gtcgaacaac gcatctacac gctcgtgccg ggcggcacgg cggaatacat ccgggtctac
    29701 gaggaatgcg ggcgcggcgt gcaggagcgc atcctcggca ggatgctcgg ctactacacg
    29761 cgggaggtgg gcgagctgaa ccagctgatc tacctctggg agttcgattc gatggatgag
    29821 cgcgcgagtc ggcgcgagcg actgttggcc gacccggact tcaaggtctt ccgcgggcgc
    29881 atccgccacc tgatggtccg ccaggagaac tcgctgctcg tgcccgccgg gccaaggcca
    29941 gcgcaggaga agaaggcatg aacgagtcgt acgcgcctcc gtcgatgtac atcgacggcg
    30001 aattcatcgg aagttccgac ggtgtcggcg acccggtgct gaatcccgcc gacggcggcg
    30061 tcctcggcac ggtgccacgg ggcacgagcg cggacgtcgc gcgcgcggtc gaggcggcac
    30121 agcgcgcctt cgaatcgtgg cgcctggaat cgcccttggc gcgatcggac ctgctgcgac
    30181 gtgtcgccgg gctgacgcgc gaacgtgccg cgcagatcgg cctgggcatc acgctggacc
    30241 agggcaagcc gctcgcggaa gccgtcggcg aggtgaccgc gagcgccgaa cacatcgagt
    30301 ggcacgcgga agagggccgc cgcatctacg ggcgcgtcgt gcctgcccgg tcgccggccg
    30361 tccgccagct cgtgctgcgc gagccgatcg gcgtgtgcgc cgcgttctcg ccgtggaact
    30421 ttccgttcaa ccaggcgatc cgcaaggtcg tggctgccct cgcgagcgga tgcacgctca
    30481 tcctcaaggg ctcgccggag acgcccagcg ccatcgtcgc gatcgcgcgg ctattccatg
    30541 atgccggcct gccgccgggc tgcctgaacg tcgtgtgggg atcgcccgag gagatctcgc
    30601 gccagctcat cgagtcgccg atcgtgcgga tgatctcctt cacaggatcg gtgccggtag
    30661 gcaagcggct ggccgcgctc gccggcgcgc acatgaagcg cacgacgatg gagctcggcg
    30721 ggcacgcgcc ggtgatcgtc tgcgacgacg ccgacgtcga tcgggccgcc gacctgctgg
    30781 caccctacaa attccgcaac gcgggccagg tctgcgtctc gccgacgcgc ttcctggtgc
    30841 agcgcgccgc gttcgatcgc ttcgtcgatc gcttcgtcga tcgcgtgggc ggcatccgcg
    30901 tcgggcgcgg catcgatccg ggcgtcacga tggggccggt ggccaatgct cggcgcctgc
    30961 aggagatgga ggcgttcgcc gaggatgcgc gggcgtccgg cggccggatc gcgaccggtg
    31021 ggacgcgcct gggtgatcag ggcaatttct tcgcgccgac cgtcgtcgtc gacccgtcgc
    31081 gcaccagccg gctgatgcgc gaggagccct tcggtccgat tgccggcatc ctgcctttcg
    31141 acgagctcga cgacgcgatc cgcgtcgcca actcgctgcc gttcggcctg gcggcctacg
    31201 cgttcacgcg ctcgaccgag cgtgcgcacg cgatcgccac gcgcctggag gcgggcatgg
    31261 tcaacatcaa ccatttcggc atggcgccgg ccgaaattcc ttttggcggc gtcaaggaca
    31321 gcggcttcgg cagcgaaggc ggcgtcgaga ccttcgacag ctacctcgtc acgaagttcg
    31381 tcacgcagct caactgacag gccagcaacc agatgtccac ccagcaagcg ctctcgatgt
    31441 cccgttgcta cgtcgacggc gaatggatcg ccgcgaccga cggatcgacg attgccgtca
    31501 ccaaccccgc caacggcgag ctcgtcggcg aagtgccggg gcttggtcgc cgtgagaccc
    31561 gactcgcgat cgaggcggcc ggacgtgcct tcatccactg gcgcgagcgt acggccgagg
    31621 agcgggcgac gctgatgcgc cgctgggcgg cgctcatgat ggcgaaccag gaggaactcg
    31681 cgaccttgat gacgcgcgag cagggcaagc cgatcgccga gtcccgcggc gagatcgcct
    31741 acgcggcctc ctacgtcgac tggttcgcgg aggaggcgcg ccgcatcgac ggggaggtga
    31801 tcccgtcgcc atggcgcggc aagcagatcc tcgctgtcaa ggagcccgtg ggcgtctgcg
    31861 cggccatcac gccgtggaac ttcccgtcgg ccatgatcac gcgcaaggcg gccccggccc
    31921 tggccgcggg atgcaccatg gtggtgaagc cggcttcgca gacgccattg tccgcgctgg
    31981 ccctggcgcg cctggccgag ctggccggca tccccgcggg ggtgttcaat gtcgtcacgg
    32041 gcagcgcctc cgcgatcggc gccgagctca ccgcacatcc gatggtgcgc aagctgtcgt
    32101 tcaccggatc gaccgagatc gggcgggcgt tggcggcgca gtgcgcgccg acgctgaaga
    32161 agctgtcgct cgagctcggc ggcaacgcgc ccttcatcgt gttcgaggac gcggacctcg
    32221 acgcggcggt ggtgggcgcg atcgcgtcga agtaccgcaa caccgggcag acgtgcgtct
    32281 gcacgaaccg catcctggtg caggacgcga tccacgacgc gttcgtgcac aagctcgcga
    32341 aggccgtttc ggagctgcgc gtgggcgatg gcctggagcc ggacgtgacg caagggccgc
    32401 tgatcgacga gcgcgcgctc gcgaagctcg actcgctggt cgaagacgcc cgaagcaagg
    32461 gcgggcgcgt cgtcgtcggc ggccgtcgcc atgggctcgg cgggacgttc tacgaaccga
    32521 cgctcgtcgt ggaggcgcgt gaggacatgg cgatcgcccg cgaggaggcg ttcggccccg
    32581 tggcgccggt cttccgcttc cgcgacgagg cggaggcggt ccgcatggcc aacgatacgg
    32641 aatacggcct ggcggcctac ttctactcgc gggacctggc gcgggcgtgg cgcgtggcgg
    32701 gccagctcga atacggcatg gtcggcatca acagcggcat cctgagcacg gcggtcgcgc
    32761 cgttcggcgg cgtcaagcag tcgggctacg ggcgcgaggg ctcgcggcat ggcatcgacg
    32821 actatgtcca cctgaagtac ctgtgcatgg gcgtcgagtg agcccggtgc tgatccgggc
    32881 taccgcgggc gggcgatcgt tcctagacta gccctcggcg atccggcatc gcgcgaaacc
    32941 gccacccggc gacgatccgg gcagacgata catgcagcgg caggcgagcc ggtgcggaga
    33001 caaggcatgg ccctcacgaa ccaattgcag gacaccgggc gcgccgaggc tgcgccgggt
    33061 gcggcgaccg gcttgcgccg cggcacgctc ggcgcgggat tcatcacgct cctggtgatc
    33121 tcggccgcga gcccgctgag cgtcgtcgcg ggcggcttcc cgctgggcat catgctgggc
    33181 aacggcgcgg gtacgcccgc gctggtggtg gccgcgctcg tgctgctgct gctgttcgct
    33241 gccgggtata ccgccatggc cacctgcgtg accagtgccg gtgggttcta cgcgatggtc
    33301 gcccaggggc tgggcggcat cgccgccggt gcggcatcga cggtggccgt gctcggctac
    33361 gtcacgctgc agttcgggct ctacggcctg ttgggcgcgg tcacctccga gtcgctcggc
    33421 tcgcagctgg gcctcgccat cccgtggtgg ggatgcgccc tcgcggccat ggccaccgtg
    33481 gcgtacttcg gctatcacca gatcgacttc tcggcgcgcg tgctggcctt cttcgtcgtc
    33541 gcggagtacg tcgtcgtgct gacgctcgac tgcctcatcc tcggacgggg cggtgccgag
    33601 ggcatcgaca tccggtcgtt ctcgccgcac gtggtctcga tgggcaaccc gttcatcggg
    33661 ctgctcttct gcttcgccgc gttcatcggc ttcgaggcca ccacgatcta cagcgaagag
    33721 gcgaaggatc cgaagcgcag cattccggtg gccacctacg ccgcgctcat cctggtcggc
    33781 tgtttctact cgttctcgct gtggtgcctg gtgctggggg ccggcgcgga tcgggtcgtc
    33841 ggcacgatct cggccctcgc cgatcccacg agcttcctct acaccctgtc ggacaggtac
    33901 gccggccacg gcctgaccac gctgctgcgc ggcatgttcg tgatcagcat ctacgccggc
    33961 ctgatcgcct tccacaattc caccgcgcgc tacttctatt cgatggggcg cgaaggcctg
    34021 ctgccggcgg cgctcgggcg gacgcatccg aagtaccgca gcccgcacgt cggatcgatg
    34081 ttgcagaccg tgctctgcgc cgtcgtggtc gtcgccttcg cggtcgccaa ggcggatccg
    34141 gtgctgacgc tgttcgcctg gctcagcaac ctggcgaccg tctgcgtgct ggtcctgatg
    34201 atcgccaccg ctgtttcggt catccggttc ttccacgtcg atccgcgcgg ccatggccgg
    34261 atgcgcacga tggtgctgcc aggcctgggc gcggtcggac tgtgcatggt gctcgtgctg
    34321 gccgtgctga acttccacgt gctcaccggc gcgagccgcg ggatctcgca cggcgtggtc
    34381 gcgttcgtgc cggtcgccat cgtcgtggga gcctggatgg ccgcgcgcct gcgccggcgc
    34441 gacccggcgc gctaccttcg cctgggacag aaccggccct gatccgcggg agcggacccg
    34501 acggacggtg tgcggccgcc gacacgcgta ggcagggcct tcgcatgtgc gtcggccgag
    34561 gatcgctcgc cggtcatcga tcggcagctc gatcgccgcg cctcgtctgc gagggcagct
    34621 cgccgaacag ctcgtggtac tcggccgcga agtggctgag atggaagaag ccccagcgcg
    34681 cggccgcgtc gccgatcgac agctcggccg cacgcgtcga catcaactcc cgtcgcacgc
    34741 cattgaggcg gatcgaccgg atgtagttga ccggcgtcgt ctcggcgacg ctgcggaagc
    34801 tgttctgcat cgtgcggcgg ctgatccgca ggcgctcgca gagctcgacc acgctcggcg
    34861 cattgacctt gtccgagatc gtcatgcgat ggcacttctc gacgatgaag ctgcgcaccg
    34921 acgatccatg ctgctgcgtg cgatcgcagg cggggtcggc aatgaggccc agcagttcgt
    34981 cgagcatcgc ctgttccagg ttcatctcga cgccgttcgg tggctcgggc agcgcacttt
    35041 ccgcagccgc ttccgagaac atcgccatca ggcgtcgccg gctcgcggcg agccgttcga
    35101 tcggaacgcg caccacgggc tgcttcagca gcgtgttgat ctcgtcgacg cagcccgtgc
    35161 tggagaccgc gttgtcgaac agcgcctcgt cgaaggtgat tgccagcatg tccatgccca
    35221 tgggcatgtg gaacatgaat tcctcgcctc ctcgcagcac gaagatgctg gcatcgtcgg
    35281 cttcgcggcc ttgcatcctc accgagccgg ggacgagcaa cggcactgcg aagcacaccc
    35341 gtccgcgcgg cgcctcgcca tgctggacga cccgctggtt gatgcgttcg cggaaggcgt
    35401 ggaagcgtgc gttgcggatc tggcacagcg agctgcgaag cggacctgcc gtgagctggc
    35461 tgtagatctg ctgccactcg cgcacggatg atgcgtgctg gtggatgtct tcgaactcgt
    35521 cgatgttcag gctcaaggct ggcctcttga agtcggtgcc cctccgggcc gcgcgcgccg
    35581 cgcgtgacca tcacgaaatt gtcatgcgca tgccagtgac cggggggtgg gtaaacccga
    35641 ttcgtggtga actgcgcatc gacgatgcgt cgttttggtc acatgcggga cgcaaacgca
    35701 caggacggcg cgaggccggc cgcttgcggc gcgcgcttgc tgatttgggc taacgcgcac
    35761 ggatggcgcg cgcagagact tcggctatcg ctgcgcacga cgcggtgccc atattccatc
    35821 agtcaaggat cccatgacca agcatctgaa gcacttcgtc gcaggcgaat ccgtggactc
    35881 ctccgacggt gcgcgcatga gcctcgtgag tccgatcgac gaaagcgaat acgcgaccgc
    35941 agcgcgcggc acggtggagg acgtccggcg agccgtcgcg gccgcgcgcg cgcaactcga
    36001 tggcggggcc tggagcaagc tgagcggctt gcagcgcggc ctgctgctga gccggctcgc
    36061 cgacctcgtc gagcgcgata ccggtctcct cgcggacatg gatgccaatg ccatcggccg
    36121 ctcgccgctg gagccccgca tgatggacgt gcccaacgcg gtcgccaacc tgcgcgcggc
    36181 ggcgggatgg gccaaccagc tcgaggggcg cacgatcccc acgtcgggct acatgggcac
    36241 gcccaccctg tcgtacaccg tgcgcgagcc gatcggcgtg gtcggtgcga tcgtgccctg
    36301 gaactcgccg ctgatgatca cgacgtggaa gctcgcggcg ctcctcgcgg cgggctgcac
    36361 ggtcgtcgtc aagccctcgg aggagacgcc gcaatcggcc ttgcacctgg cggcgctttg
    36421 caaggaggcg ggctttcccg acggcgtcgt gaacgtcgtg accggctacg gcgacgtggt
    36481 cggtcgcgca ctgtgcgagc accccgacgt ggccaagatc agcttcaccg gcagtccgga
    36541 ggccggtcgc gcaatccagc ggatcgtcgg ccccatgttc aagcgcgtcg cgctggaact
    36601 cggcggcaag agccctcaga tcgtgttcga cgacgcctcc ttcgacgacg cggtacgcgg
    36661 ctgcacgttg ggcctgttcg tcaaccaggg ccaggtctgc gccgcaggct cgcgcatcct
    36721 cgtccaacgc gggatcgcgc agcggttcgc cgcggcgctg gcagaggcgg cctccgtcgt
    36781 gaaggtcgga gatccgcgcc agcccggcgt gcagatgggc ccggtggcca agaaggcgca
    36841 gtacgaacgg gtgaaccgct acatccagat cggcatcgac gaaggcgcgg aactgctcgc
    36901 cggcggcgtc gcgcagtcgg agcgtggatg gttcgtgaag cccacgatct tcgcgaacgc
    36961 gtccaacacg atgaccatcg cgcgcgaaga gatcttcggg ccggtcggca cggtgatcgc
    37021 gttcgatacc gaggacgagg ccatcgcact cgcgaacgac tccgactacg gtctcgccgc
    37081 cacggtctgg acgacgagcc tcgcgcgcgc gcaccgcgtg gccggtgcgg tgaaggcggg
    37141 cgcggtcggc gtgaactgct ggagcccgct cgatgcgaac cttccgtggg gcgggctgaa
    37201 gaccagcggt atcgggcgcg agggcggcct cgccggcgcc atcgcgtaca ccgaggaaaa
    37261 ggtcgtcacc gtactgctgc catgacaccc tgtgcatgct ggagcagaag atgcctacga
    37321 tgagatggcg caacacgctc gaggcctatg gcagcgtgtc gatgctgttc cactgggtca
    37381 tcgccgcggc cttcgtctcg gcctatgtcg tcgtgtacta cgtgatctgg ttcgtcgacc
    37441 cggagacgag cgtcaagccg gcgctgttcg gcacgacgcc ggacccgacc cgcgtggtgc
    37501 cgatcctgaa catccactgg atcctggggg tgacgatcgg cgtcctcgcg ttgccgcgac
    37561 tgttgtggcg gctcgcgggc ccggtcccgc gccacgttcc cggctcgcgc gtggagcacc
    37621 tggccgcaga tgcggcgcac tgggcgctat acggcctgct gatcctcatg ccggtctccg
    37681 gctacatgac gacctacgac cccacgaact tcggcctgtt cgtgattccg ccctgccgcg
    37741 cgacggcctt cgcggcctgg cttcgatcca cgttctcgct ctcgacgaaa gaactcgagg
    37801 acctcgcatg gtccgtgcac agcttcctcg gccaatggat cgcatggccg ctcgtggtgc
    37861 tccacgtcgc tgcggcgctc gtgcaccact tcgtgcgcaa ggatggcgtc ctgcggcgga
    37921 tgctgcccgt cgcctgcgac gcctcccgca gcccttgagg acatgagtac ggacgcacga
    37981 agagcggtca tcgcacttca tcgcgcgagg gatggcgcgt cgcaccggcg ttggctctat
    38041 gacggtgagc gcgcaacccg gttccgcccc gcccgcttgg cttcgtacag cgccgcatct
    38101 gccagggcga ggtgatgctc ggggttgtct ccttcccgag gaacgatcgc ggtgatccct
    38161 atgcttgcgg tgaccacgcc gtgatctgac ttctcgtgcg ggaggcgcag cgaagcaatc
    38221 tcggatcgca agcggttgca cgcagattcg gttcccgcga tgtcggtgct cgggagcacc
    38281 agaacgaact cctcgccccc atagcgcgcg acgaggtcgg cagtcatgcg cagaccgccc
    38341 ttgagcgtgg cagccacccg tcgaagacag gcgtcgccgg cctggtgacc ataatggtcg
    38401 ttgtagagct tgaactgatc gatgtcgatc atggccagcc cgatgggtgc ccccgggcgc
    38461 atggcgcgca gccactcggc ttgcaacacc tcagcgaagt gccgtcgatt cgagatcccg
    38521 gtcaacgggt cggtcaccgc gagcagcgcc aggttgcggt tagcctcctc gagcgcatcg
    38581 gttcgctcgg ctaccttgcg ctcgagcgat gcgtacacca gcgcgttgtc cagcgacacg
    38641 gccagctgac cggcgatgag caacaccgca tcgagccgag ccgctgaaaa tgcactgctg
    38701 ctgagacggt tctcgagaat caccatcgcg cgtgggacgc tctggctgtc tattggcacc
    38761 acgagcagcg agcactgctg ctgcccggcg aaatagggat ccgttgcgaa gcgaggatcg
    38821 cgcaccgcgt cgtccaccaa cagcggttcg cgaatgcggt ctgcatatcg gaatgccgtc
    38881 aacggcagcc ggccgttggc tgccgcctgc gcgatcggga tcgattgcga catggcactg
    38941 tcgggcgccg ggctcagcag gcaccactcg tgaagctggt cgtcccaaac ggccacggat
    39001 acgccagttg ccccggtcat cgcaccgaga agttcggtga ctcgtaccgt caggcgtgcc
    39061 aggctggtct cggagctcaa cgcctgcgaa gccctcagaa cgccgagcag gtctatcgca
    39121 tcggaggtca tgatgacgct cgtcgcgctg cggcgcgatt cgagtgaacg tcctgccacg
    39181 ccatgcgagc ctaggaagcc gtgaacgcgt tccatgtggc gcaccttgcc caatgctccc
    39241 caggtcgcat agtgtgtccg ggcttcgttc agaagcctct ggccggtatg caggagtccc
    39301 tgctccaggt gaaggagccc ggcccgctcg gcgatgaacg catggtgcca agggcggtgg
    39361 cgaggcgcaa cctcgcgcat ggccacatcg tagaggcgtg ccgcggcatg aaaatcgcct
    39421 tgtgcgcggg cgcgctctgc cttgatgagc agggccaggt gaccgaagtt ggccggcgcg
    39481 tctgcggcgc gctcctccca ccaaccctgc agggaatcga gctgtgcgca aatccccggg
    39541 tgttcatgcg gcaccatgtc cagacgccgc gtcagcgcca aggcgtagag cagtttggac
    39601 tgcgcggatg tatagaagca ttcggtgcat tcctcatgct ggaatgccgc tacggcgtga
    39661 tgcagcagcg cagcgtcatc gccaaacaat gcggccgcga ggccgcaagt cacgtgttgg
    39721 agtgcggttg caaaagggtt gcgaccccct tcgttgaagc atggcaagtc gacgaacggt
    39781 gcgtccgatg ccgtgtcctc tggcgcccga cctcgcagct tgtgggccaa ctgcctttga
    39841 agtacatagg aatcgccggc atgctgattt gcggtgcgct ccgcgagggc aatggccgcg
    39901 tcaatttcct ccagagaatc ttcgagcagc ggtgcgctgt cgaacagggc ggaggtcgac
    39961 gacaaatacg tgaagcaggc gttctgcaga tctccgcctt gcagcagttc ttcgcgggca
    40021 cgtcgcgcat ggatgacggt ctcttcgagc gggtcaaacc agtgactggc ggtgaaggcg
    40081 aacaggaacc gggcctgcgc ggcttgatgg gaatagcccc ttgcctcgct cacttccagg
    40141 acgtggcgaa cgtcgtcgta cccccttgcg atagtcgccg tgaaagacgg tcacaaatcc
    40201 ggtgtggcac agcggtccga ccaactccgc gcatgggccg ctttcgatcc acatggcacg
    40261 cgactctcga accatccagt ccatgctgtc catggtcaag gcggccggca gcaagcggtt
    40321 gatgatcttt gcgctggcca cggccttggg atcggtcatc tccggccggc ccacatcgtc
    40381 ggcatgcggc ggcagttccc gaaacccggt gacggcggcg gacttgggcg cttcgtctac
    40441 atgcgcgccc aggcgctgga gcatctggat cccaagggcg agggcttctt ccaggcggtt
    40501 gcggttgcat aagctgctga tctgaacgca ggcggcatcg gccagttcaa gtggcgaggc
    40561 atcgcgcgcc gcgatgtgtc ggaagagtcg gtcagcgtct tccaagcgcc ccagcgcgta
    40621 gagcgcagca tgcaaatcga cgtcgatctc ccaagccagg agttcgtctg ctcgatcgtc
    40681 tccttccagc agcgctttcg cttcggtcaa ccaacgcccg actgcggcgt agctgaccgc
    40741 ctttcgtgcc gttgcagccg catgctggaa cagccttgct acctggcgcc gttcatccgc
    40801 gtctcggacc gcgtcggccg aaaggagata ctgctccgcg gcctgcaggc agaactccgg
    40861 atgattcgcc agtcgtcgag ccagggtcag ctgggcccgt ccacgctgct gaggttcgag
    40921 gcgactataa gcggcctgtt gcacgcgatc atgccgaaag cgccatccga tggtcgttcg
    40981 ggcgtcggtg cccaacatgg gagtgagcag tccctcttcg agaggctcgc gcaacatcga
    41041 cacgagcgcg tcggctccaa ggctcgtggc cgcacgcagc agcgacatct cgatcgaatt
    41101 gcccaggcag gccagggtct ccaacagggc gcaggtttcg ggaggaagct tgccgatgcg
    41161 ggcgaagatc aggtccagta ctgtcccatg accgatatag cggcggatct cattcgcatc
    41221 ccaccgccaa ccgtcgtcgc cgcactgcag aatgccgtcg cggcgcaagg cgttgatcaa
    41281 ttcgacagtg tcgaaggggt tgcccgcggt cagcgttccc agcgcttccg acagccgcgt
    41341 ggcctccggg gagggaaggc gcagcatctc ggagacgagt tcggcgaacc ctgggcggca
    41401 ggttccccag cgacagcggg tgcggtgcga cgggagagcc gtgccaacgc acaagaagct
    41461 gcgccaatgg atgggctgcg tcgatctcgg catcgcggta cgtggccacc acgagcagcc
    41521 cgcgaatgtc tgcgtcggag atcaggcgct cgatcagccg aagcgaggtc ggatgcgccc
    41581 attgcaaatc ctcgatgacg atgacgatgg gacgtgcggt cgaggcaacg gcgcccagca
    41641 agtcgatcac cgcctggatc agacgggctt ctgcctcgac gggactgact tctccgcggt
    41701 cggcctgcgg accgagcagc gtgaggaact cgggcaacgc atcggtgagg atgccggcgt
    41761 tggacccgag tgacgtgagg atgcgttggc gttggcgttc gagctcggct ggcggctccg
    41821 ccaggagcag ccgcccgagt tcgcgcatgg cgctcctgaa tgcagtccag gccgcctcct
    41881 ggcgatattg ttcgaattta ccaatgacga accagccacc tcgggtggtc acgatggtcc
    41941 ggagttcgcc gacgagcgcg ctcttcccga cgccgggcga gcctgagaca agcacggcgc
    42001 accgggctgt gcgcacggag gcgtcgacgg cgtcgctcag agccagaatt tccgcatcgc
    42061 ggccgatcag gcgggaggga gctgtcaggt gggtggggaa gtcacgctca ccgagcgcga
    42121 acggggcgtg gctgttggat agccgggtaa ggtcgcgcac caaaccgtcg gcgctctggt
    42181 aacgatggtc cggctccttt gccagcaacc gcatcaggat gtcggacagc atgtccggca
    42241 cggccgggtt gagctgcgag ggccggtacg gcatacgcgc caagtgatcg cgaatcagct
    42301 ggagcggatc cgtgccttca aaaggaggcc gaccggcgac gacctcgtaa agggtggcac
    42361 ccaacgaata gaggtcggct cgctggtcga ctgaccgact ggtgcgccct gtttgctcgg
    42421 gagccaggta cggcagcgtt cccgtgatgg cgctttcgtg agtgaatgtg ggccgctctt
    42481 ccgccagact gcttgccagg ttgaaatcga tgagcgttgg ccgccaatcg ggccccgaaa
    42541 ccaggatgtt ggccggattg atatcgcggt gaacaatgcc ttgcgagtga acatcggcca
    42601 cgattcgggc gagcgccacc gcgaactcga tcttgtgctg gatggtcagg gactggccgc
    42661 ccagcgcttg cgcgagcgaa atgccggaga cgtcttcgag gacaagagtg cgtgtttcga
    42721 cctgggcgcc gacaaggtgc gccacgccgg cgaccgacga catccgtgcc agcatggcct
    42781 gttcgtgtcg aagccgatgt cctgcctcag gccccagcag ttccttgcag acgacccacg
    42841 tgccgtcgcg cttcagccga cggcgcacga ttcgcgtgcg ctcgctgtgg taaagcacat
    42901 cctcgcaagc tggcgctgtc gacaggtcga gtgctgtgac cgaaagggcg ctggctgaca
    42961 tggagaaagg ctggttgcgg cagagtcggg atgagccggc ggtgccgggg ctcgtcgtct
    43021 cttcggcgct gcagcggcca ccttgagagg ctgtccggca tgggtcgtgc tgcatttgtc
    43081 gcttgcgcaa gggcggcgct gccgagctgc acgcggtgcg gctcacgctc gcgacgtttg
    43141 aattattcgg tggattcgcc cgaatactgg acttgtccac tatcggtgcg tgatgggatg
    43201 gagctccatt cagagaattg accgttcaac tgagcggatc gttatggtgc ccctcaagca
    43261 gctcgccgcc agggcgaaat cgggccatgt acacgccgac gcgccgtccc tcggcagcgg
    43321 cccggcgacc gttccgcctc gtcgactcga ggcatcgcag gagactgtca tgacccattc
    43381 ccaccatcgg cacgccatgc catccctgat tctgcgctgt gcatggctgc gcgcggcgcc
    43441 gacgccgccg atttcaagtg gatccaggat cccggaagct gccggtcgcc cgcgtgcctt
    43501 cgtgcctccg tggatcgcga cgttcaagcc ctggcaggcc gacaccagac ttgcgcggca
    43561 atgggtgagg gccgagttcg tctggcaact caactgactg cgcggagacc cacatgcgac
    43621 tgacccacct caagatcggt gtccggctgg cgctcgcgtt cggcgctgtg ctgtgcctgc
    43681 tgtcgatgct ggctaccgtc gcggtgatgc gcaccgacgg cctgcgcgaa gacgtcaagg
    43741 ccctcaccgc cggcatcgcg gcggagtccg cgcttgccga ccgcatgcgc ctcgccgcga
    43801 cgcggcaggc cgtgcgcgtg cgcaacgtcc tgatggtgtc cgagccggag gaaatggccg
    43861 ccgaggaaca gctcctgaag gccgacacgc acgactacga cgcggccaag ggcagcctcg
    43921 cacgcctgat cgggcgcgat gtcgacccgc gcaagagggc catcctggcg cgcatcgacg
    43981 cggcggagga ggcgcgcgat ctctccggtc aggtcgtgaa gatggcgctc gagaaccaca
    44041 agttcccggc ccgcgggctg ctcggtgaga aggcaaaccc ggcacaggcg aagtggatgg
    44101 ccgcggtcga cgagatggtc gcgctcaacg agaagctcag cgccgaggcc gtcgcgggtg
    44161 tcgagcaggc ctgcgccaag acgcgcgaca tcgtgcttgc gatctcactg gctgcgctgt
    44221 tcgtggctgc ggtgatcggc cacgcggtca cgcgttcgat cacggtgccg atctcgcaag
    44281 ccgtggacgt ggcgcgccgg gtcgcggccc gcgacctgag cgtgcgtgtg gacagcgacg
    44341 ttcccgacga gaccggccag ctgctgcggg ccctcggcgc gatgaccgag agcctgctca
    44401 cggtggtctg cagcgtgcgt gccagcagtg acaacctggc cggcgcagcc atcgagattg
    44461 cctcgggcaa cgccgacctc tccaaccgca ccgagcgcca ggccgcgagc attcagcaga
    44521 cggcctcgtc gatggaggag ctctctggca cggtgcgcac cagcgcggcc aactcgaccc
    44581 atgcgcgcca gttgtccacc gaggctgccg atcgcgcccg ggagggcggc gacgccgtcg
    44641 gcaaggtggt tcgcacgatg ggagacatcg tcagctcgtc gcgccggatc gccgacatca
    44701 tcggcgtcat cgatggcatc gccttccaga ccaacatcct ggcgctgaac gcggccgtcg
    44761 aggccgcgcg cgcaggcgag cagggtcgtg gcttcgccgt cgtcgccacg gaagtgcgca
    44821 cgctggcgca gcgcagcgcg accgccgcca aggagatcaa gcagctgatc gccgaatcgg
    44881 tggacaaggt tgacgagggc acacgcctgg tcgacgtggc cgccggcacc atcgagcagg
    44941 tcgtgtgcgc ggtgcagcgc gtcgccggca tcgtgtcgga gatcacctcg gccacggtgg
    45001 agcagagcgc cggcatcgat ctcgtcaacg acgccgtcgc gcagatggac cacgccacgc
    45061 agcagaacgc ggccctggtt gagcaggtcg ccgcggccgc cgactcgatg cgctcgcagg
    45121 tgcagcggct ggttggcgat gtgagcgtgt tcagggtctg agaatccggc gggcaacggc
    45181 ggggcggacg ggacgctttg cagccgtaac cgagcgcgcg tcgccgccgt caccggacgc
    45241 ccgccgcgag atcgaggcct gtcgcccagc aggagcgcga gttgatggat gccttgcatg
    45301 tccagcgcat ggctgtgacc ggctcgacca agctcgcgtc gacgcaatcg aatcctccct
    45361 gctcggtctc ctggattccc tgttattcga taacggacgc cctgttccaa gcctgaccga
    45421 attgcgtatc ggccgcgcat aagcccgcgg cggcattgaa tttttccaaa tcacgggagg
    45481 tactgcttcc cgtccgcatc ggaaattcgc tgttttcgtc gagacgggtt cgcacctgcc
    45541 tccccgcacc gccaggaagc aagcaaacac agggcctcgg ccctgttttg tttcagttgc
    45601 gccgatcgtc accgatcggg cctgcgcacg cccgcggcgg ccggcgcggc gaggcgcgcg
    45661 gcatgtcccc cgcaagaggg attcgcggtc gcacgggcct cgttagagtt cgcgccttgt
    45721 cgcacggtcg agctggctcg cagccgccgg cgatctccca accgcgaacc gacacgatgc
    45781 tcccgaaacc tctgcgcgcg acttccctgg cctgtctggc ggcgctcgcc gcctgtggcg
    45841 ggggcggcgg ctcgccggcg gcgcctgcgc ccgacaccca cgccatcacg atcaccggcc
    45901 agcccgtcgc cgcgacgccc gcggcggacg gctcgcagac ctggtcggtc acggcttcga
    45961 gcgacatcgc gctgtcgtac cagtgggcga tcgacggccg cgccgtcgcc ggcgcgacga
    46021 gcccgagctt cacgatgacg cccgccctgt cggacgacgg cgagcaggtg agcgtcacgg
    46081 tcagcaactc ggccacctcg gtcgtcagcg ccgcggtgac cgtctcggtg gccgcggtga
    46141 cggggctcaa cgtgttcgcc ggccgcatcg gcggcgcggg ctacgtcgat ggcgccgcgt
    46201 ctgtggcgcg cctcgggcac gtggagcagg ggtggagcgc cagcgcgctc ggcaccgacg
    46261 gcgcgggcga cctgctggtc gtcgacccgt ccaacttcgt cgtgcgccgg gtgacgccgg
    46321 cgggcgtcgt gtccaccgtc gcgggcgtcg cgggccattg gggctcgacg gacggccccg
    46381 tcgcctcggc gctgttcccg agcgcctcgg gcgtggtcgc cgacgccgcc ggcaacgtct
    46441 acgtctcgac ctatcgcgag atccgcaaga tcaccccggc gggcgtctcg accctggcgg
    46501 gctccgaatc cacgagccgc gaggtcgacg gcaccggcgc cgcggcgggc ttcgatgccg
    46561 tcctcgggct cgccatcgat cccgcgggca acctgtacgc ggccgacttc gcggcatcga
    46621 tccgcaagat cacgcccgcc ggcgtcgtga cgtcctacta cgacgcctcg aagcatccgg
    46681 gccagcgcag cttccagtcg atggtcttcg gcgcggacgg caacctctac gcggtcgacg
    46741 ccatgagcga cgccctcgtg aagatctcgc cggcgggggt gatcacgaac gtcgccggcg
    46801 gcatcgtcga caaccccgac ggctcggtca gcgtgggccc gttcggcggg cagagcgacc
    46861 tcgcgatcgg tgccgacggc gtcctcacgt cgttcaacct cggctcggcg acgacgccgc
    46921 cgacgatgac gcgcgtggcg gccgacggca ccaccagcac cgtcgcgctc gacctgccgg
    46981 acaacgaccg cgtcggcggc gcggtggcgt tcggcccggg cttcgcgctg ctggaccaga
    47041 cccagggcgc ggtctacctg gtcgacacct ccggcaaggc gaccctgttc gtcggccagc
    47101 cgccgcggag cggcaacgtc gacgggcgcg gaggcgcggc cacgttcgat cgccccggca
    47161 aggtcgtcgt ggacgcggcg ggcaacgcct acgtgctcga gaccgacggc tacgcgacca
    47221 accacacggt ggtgcgcaag gtcgccaccg acggcacggt ctccaccgtc ttcgccagcg
    47281 aggactggta cggcgccatc gccgtcgacg cgggcggcca gctttacggg acgcagtacg
    47341 acgaggtcga caagctcaac ccggacaaca ccgcgacggt cgtcgtcgac cgctcgcagc
    47401 tggccgcggc cggcggctcg aatcccttcc ccgcgctgcg cgacctggcc ctcgcgcccg
    47461 acggccacct cctggcctgc acggacgccg gcctgttttc ggtggcggtc gccgcccgga
    47521 cggtcgccgc cctgaacgcc gtcgccaact gcggcgccgt ggcggcgacg gccagcggcc
    47581 ggatctacga gctgcagacg gtctatgcca cgacggccgc cgcggactcg gtgctgctcc
    47641 tggacgcgac gacgcccggc accccgaccc tcgtggccgg cggcaccgtg gggtacgccg
    47701 acggcacggg cgccgcggcc gcgttcggcg atttccagct cggcgcggac gccactggcg
    47761 acaccgtgcg gggccgggtc gcgttgagcg ccgacgagga cggcaacctg tacatggtcg
    47821 acccggacaa ccacgccgtg cggatgatca cgcccgccgg ggtcgtgacc acgctcgcgg
    47881 gcggcccgac gacgatctcg caggtcgtga ccggcgcgac cgccggcctc gactacccgg
    47941 gcggcgtcgc gcccctcccg ggcacgggcg gcaggcagct gttcatcacc gatacgtacg
    48001 agccgtcgct gctggtgttg cggcggccct gacgggttcc cgtcgcgggc cgcggcacgg
    48061 tccgctggcg gagggtctcg ccacatccac cgcgcgccgt atgcccggtc gctgccggcg
    48121 aagcgccgcc tgacaagcct ggccaccgtc gcgtcggacc ggcggcggcg cgcccgcggc
    48181 aacgacggcg ggcgccggac gcgcgaagcg ccgttcgggc gcgcgaagcg gcgggccggt
    48241 gtcatgggat cgtcatgaag cggggccggg ggctcgccta ggctggctgc atcgtcacga
    48301 cgccttcccc ccgccatgcc acgcctcgac atcgactccg accgccagcg tcgcctgtcc
    48361 atctcctccg atccggcggg caagcgcccc gcgctcccgt cgaacgaggg cg
//
LOCUS       DASVLN010000461         6365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_293147, whole genome shotgun sequence.
ACCESSION   DASVLN010000461 DASVLN010000000
VERSION     DASVLN010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293147"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VF453_11340"
     CDS             <1..262
                     /locus_tag="VF453_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688295.1"
                     /translation="REAARLRIARGPGDHAATRSHRGRDVALHGQGAREEGWASEPDD
                     VGGRARIPEPDPAVAPGTASLDEAAPRSGAALRSAGDRPLEP"
     gene            446..934
                     /locus_tag="VF453_11345"
     CDS             446..934
                     /locus_tag="VF453_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7688296.1"
                     /translation="MTPRLPHVACTALAALLALAALARAHAAPAAPTAADLEVIATQV
                     RAAETAFAQTMADRKLDRFAGFVAEDAVFRGATLRVGRAQVVEGWKRFFEQPAAPFSW
                     APDLVTVAADGRTAISTGLARAPDGTPISRFTSIWRRDADGQWRVIADQGVDAACDGR
                     GK"
     gene            complement(955..3051)
                     /locus_tag="VF453_11350"
     CDS             complement(955..3051)
                     /locus_tag="VF453_11350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7688297.1"
                     /translation="MTAHNPLLADWNTPFSLPPFAEIRAEHFAPAFEAAMAAQRAEIE
                     AIAASPGAPGFDDTIAAFDRSGRLLGRVGAAFHALAASHTSPEIQAVQRELAGPLAAH
                     ESAVMMHPGLFKRVDAVHARRHELGLGPEALRLVERTWVDFVRAGARLGPDKQPRYAQ
                     IMERLAQLTTRFAQNVLADEAGFQLELSQADLDGLPPFVRAAASQAAAERGLPAGAHV
                     VTLSRSLIVPFLTFSTRRDLREQAWRAWVSRGEHEGETDNREVAREILRLRQEQAWLH
                     GHASYAEYALADTMAGNVASVNTLLDEVYSRALSALKDERAAVHEALREAGVPGPMMA
                     WDWRFGAERVRQARYAFDDGEVKPYFSLERMVQAAFDCAHRLFGLRLVRNDAIPVYHP
                     DVVAYEVHDVGVPGEGAESPQAGRLVGIFLLDNFARQTKRSGAWMSALRWQCRNGGVD
                     LPVIMNNNNFAKGAPGEPTLLSFDDARTLFHEFGHGLHGLLSDVTYQRLSGTNVLRDF
                     VELPSQLYEHWLLEPEVIARHARHWQTGEPIPESLVRRLQEARRWGQAYETVRYAGSA
                     LVDMAVHARLDKEPPPDLPEFEAETLAKLGLPPEVGVNHRLTHFQHLFSGSGYAAGYY
                     VYLWAEVLDADAFDAFKEAGGPFDRATADRLRRCIFGAGDSVAPQQAYADFRGRMPSI
                     EPLLRKRGLLPEDIEY"
     gene            3247..5331
                     /locus_tag="VF453_11355"
     CDS             3247..5331
                     /locus_tag="VF453_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7688298.1"
                     /translation="MTDPATPASTASAGDDPFLWLEDVLGDEALAWVRARNGESRAVL
                     ESEPRFAEVRDAIRAILDSKEQIPHVTRRGAWFYNFWRDEAHPRGLWRRATLDEYRKP
                     QPAWEIVIDLDALAAEEDENWVWAGAHALAPAYRRCLVTLSRGGSDAHVVREFDLELR
                     RFVPGGFDLPEAKSDVTWIDDDTIYVASDFGPGSLTDSGYPRVVKRWRRGTPLEAAPT
                     VFEGEAADVAAGVSVDRTPGWERTVFSRAIDFYNSRLALLDPASGALVAIDKPEDATL
                     SFWRERVLLELRSDWTLPEATWPRGSLLVADAAAYLRGERRFEALFTPTATRSLAGFG
                     VTRSRVMLDVLDDVAGRIVELSPGPDGWTRREVAAPFPGALSTTELHDPTLAEDPLAE
                     SYLLGYTDFLTPDSLYLAEAGSDARELLKARPSYFDGAGMRVEQRFARSADGTRVPYF
                     VVWPAGATADGDNPTLLYGYGGFEVSLTPWYSGAFGRAWFARGGVLAVANIRGGGEYG
                     PAWHQAAVKEHKQRSYDDFIAVAEDLVAAGITKPARLGIEGGSNGGLLVGAVMVQRPE
                     LFGAVSCQVPLLDMRRFNKLLAGASWMAEYGNPDALDDWAWISRYSPYQNVHAGRRYP
                     SVLFTTSTRDDRVHPGHARKMAARMLQQGHDVLYYENIEGGHGGAADNAQRADLQALQ
                     FAFLWRKLGMKR"
     gene            5416..5694
                     /locus_tag="VF453_11360"
     CDS             5416..5694
                     /locus_tag="VF453_11360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7688299.1"
                     /translation="MTYEIRHDPARQRFETTVDGQLCVADYQLRGDTVWMTHTGVPQA
                     VEGRGIAAALVRTALDWCAAQGFRVEPSCSYVAVYMRRHPETQRLLAR"
     gene            complement(5888..>6365)
                     /locus_tag="VF453_11365"
     CDS             complement(5888..>6365)
                     /locus_tag="VF453_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEX7688300.1"
                     /translation="FLAGHLARPWIAGVIARRKEIVGIVDRGSILLVVYTAFSAAVIE
                     GLWKQVPWQSLVGLFVVCCVLLALVLFATGRIARALGFDRADQITIVFCGSKKSLASG
                     IPMAKVLFAAHAVGALVLPLMLFHQLQLMVCAVLAQRWGVRRDEAARVPAAASSRA"
BASE COUNT          867 a         2395 c         2295 g          808 t
ORIGIN      
        1 ccgggaggcc gcccggctcc ggattgcgcg gggcccaggc gaccacgccg cgacgcgctc
       61 gcatcgcgga cgagacgtcg ccctgcacgg ccagggcgcg cgagaggagg ggtgggcgtc
      121 ggaaccggat gacgtcggcg gacgcgcgcg gatccccgaa ccggatccgg cggtcgcgcc
      181 gggaacggcc tcgctcgacg aggcggcgcc gcgctcgggc gccgcgctgc ggtcagcggg
      241 cgatcgtcct ctcgaaccgt gacgtcacgg cagtcgacgc gctgagcagg gtcttgtcca
      301 tggcgttctc cttggcggct cggttgaggg aaagccgcga ctgtgcgccc gtgggcgcgc
      361 cgctgtcaag atggaaacca gggccgccgc ggccgtgtcg cgtctcccac aatcggccgc
      421 atcgtcgacc tggagcccgc ctgccatgac gccccgcctg ccgcacgtcg cctgcaccgc
      481 gctcgccgcc ctgctcgcgc tcgccgccct cgcccgggcg cacgccgccc ccgccgcgcc
      541 gacggccgcc gatctcgagg tcatcgcgac ccaggtccgc gccgccgaga ccgcgttcgc
      601 gcagaccatg gccgaccgca agctcgaccg cttcgccggc ttcgtcgccg aggatgcggt
      661 cttccgcggc gcgacgctgc gcgtgggccg ggcgcaggtc gtcgagggct ggaagcgctt
      721 cttcgagcag cccgccgcgc cgttctcgtg ggcgcccgac ctcgtgacgg tcgccgccga
      781 cggccgcacc gcgatctcca ccggcctcgc ccgcgcgccc gacgggacgc ccatctcccg
      841 cttcaccagc atctggcgcc gcgacgcgga cggccagtgg cgcgtgatcg ccgaccaggg
      901 cgtggacgcg gcgtgcgacg gacgggggaa atgacgcgct gcgcctgctg acggtcagta
      961 ctcgatgtcc tcgggcagca ggccgcgctt gcgcagcagc ggctcgatcg acggcatccg
     1021 gccgcggaaa tcggcgtagg cctgctgcgg cgccacgctg tcgccggcgc cgaagatgca
     1081 gcggcgcagg cgatcggcgg tggcgcgatc gaacgggccg ccggcctcct tgaaggcgtc
     1141 gaaggcatcc gcgtcgagca cctcggccca gaggtagacg taatacccgg cagcgtatcc
     1201 ggatcccgag aacaggtgct ggaagtgcgt gaggcggtgg ttgacgccga cctcgggcgg
     1261 cagccccagc ttcgcgagcg tctcggcctc gaactcgggc aggtcgggcg gcggctcctt
     1321 gtccaggcgc gcgtggacgg ccatgtccac gagcgcgctg cccgcgtagc gcacggtctc
     1381 gtaggcctgg ccccagcggc gcgcctcctg cagccggcgc acgagcgact ccgggatggg
     1441 ctcgccggtc tgccaatggc gcgcgtggcg cgcgatgacc tcgggctcga gcagccagtg
     1501 ctcgtacagc tgcgagggca actcgacgaa gtcgcgcagc acgttggtgc ccgacaggcg
     1561 ctggtacgtc acgtccgaca gcaacccgtg caggccgtgg ccgaactcgt ggaacagcgt
     1621 gcgcgcgtcg tcgaagctca gcagcgtcgg ctcgcccggc gcgcccttgg cgaagttgtt
     1681 gttgttcatg atcaccggca ggtcgacgcc gccgttgcgg cactgccagc gcagcgcgct
     1741 catccacgcg cccgagcgct tggtctggcg cgcgaagttg tcgagcagga agatgccgac
     1801 cagccggccc gcctgcggcg attcggcgcc ctcccccggc acgcccacgt cgtgcacctc
     1861 gtaggcgacg acgtccgggt ggtagaccgg gatcgcgtcg ttgcgcacca ggcgcagccc
     1921 gaacaggcgg tgcgcgcagt cgaaggccgc ctgcaccatg cgctccagcg agaagtaggg
     1981 cttgacctcg ccatcgtcga aggcgtagcg cgcctggcgc acgcgctcgg cgccgaagcg
     2041 ccagtcccag gccatcatcg ggcccggcac gccggcctcc cgcagcgcct cgtgcacggc
     2101 ggcgcgctcg tccttcagcg ccgacagcgc gcgcgagtag acctcgtcca gcagcgtgtt
     2161 gaccgaggcg acgttgccgg ccatcgtgtc ggcgagcgcg tactcggcgt agctcgcgtg
     2221 gccgtgcagc caggcctgct cctggcgcag gcgcaggatc tcgcgcgcga cctcgcggtt
     2281 gtccgtctcg ccctcgtgct cgccgcggct cacccaggca cgccaggcct gctcgcgcag
     2341 gtcgcgccgg gtggagaacg tgagaaaggg cacgatcagc gagcgcgaca gcgtgaccac
     2401 gtgcgcgccc gcgggcaggc cgcgctcggc ggccgcctgc gacgccgcgg cgcgcacgaa
     2461 gggcggcagg ccgtcgaggt cggcctgcga cagctcgagc tggaagcccg cctcgtcggc
     2521 gagcacgttc tgcgcgaagc gcgtcgtcag ctgcgcgagg cgctccatga tctgcgcgta
     2581 gcgcggctgc ttgtccggcc cgaggcgcgc gccggcacgc acgaagtcga cccaggtgcg
     2641 ctccacgagc cgcagcgcct ccgggccgag ccccagctcg tgccgccgcg cgtgcacggc
     2701 gtcgacgcgc ttgaacaagc cggggtgcat catcaccgcg ctctcgtggg ccgcgagcgg
     2761 gccggcgagc tcgcgctgca cggcctggat ctcgggcgag gtgtgcgacg cggcgagcgc
     2821 gtggaacgct gcaccgacgc gcccgagcag ccgcccgctg cggtcgaagg cggcgatcgt
     2881 gtcgtcgaag ccgggcgcgc ccgggctggc ggcgatcgcc tcgatctcgg cccgctgcgc
     2941 cgccatcgcc gcctcgaacg caggagcgaa gtgttcggcc cggatctcgg cgaacggcgg
     3001 cagcgagaac ggggtgttcc agtcggcgag cagcgggttg tgggcggtca tggcgggatg
     3061 ggctccgggc gagggattcg cgaggcgggc gatgatgcta gcgtcgccgc tgcgcgcaca
     3121 ggatacgggc gtgcccgaac cccacgctcc ccgcgaggaa attcgcacgc gcggccccgc
     3181 cgcggacagg cgcggcgcgc tctgcgatga tggccgcctt cccgcgccac gctgccgatg
     3241 cccgccatga ccgatcccgc cacgcccgct tccaccgcct ccgccgggga cgatcccttc
     3301 ctctggctcg aggacgtcct cggcgacgag gcgctcgcct gggtgcgggc tcgcaacggc
     3361 gagtcgcgcg ccgtgctcga gtccgagccg cgcttcgccg aggtgcgcga cgcgatccgc
     3421 gcgatcctcg actcgaagga gcagatcccg cacgtgaccc gccgcggcgc gtggttctac
     3481 aacttctggc gcgacgaggc gcacccgcgc ggcctctggc gtcgcgccac cctcgacgaa
     3541 taccgcaagc cgcagccggc ctgggagatc gtgatcgacc tcgacgcgct ggccgccgag
     3601 gaggacgaga actgggtctg ggccggcgcg cacgcgctcg cgcccgccta ccggcgctgc
     3661 ctcgtgacgc tctcgcgcgg cggctcggac gcccatgtcg tgcgcgagtt cgacctcgag
     3721 ctgcgccgct tcgtgcccgg cggcttcgac ctgcccgaag ccaagtccga cgtcacctgg
     3781 atcgacgacg acacgatcta cgtcgcgagc gacttcggcc ccggcagcct caccgactcc
     3841 ggatacccgc gcgtcgtcaa gcgctggagg cgcggcacgc cgctcgaggc cgcgccgacc
     3901 gtgttcgagg gcgaggccgc cgacgtcgcg gccggcgtct ccgtcgaccg cacgccgggt
     3961 tgggagcgca cggtcttcag ccgcgcgatc gacttctaca actcgcggct cgcgctgctc
     4021 gatccggcga gcggcgcgct cgtggccatc gacaagcccg aggacgccac gctcagcttc
     4081 tggcgcgagc gcgtgctgct ggagctgcgc tccgactgga cgctgcccga ggccacgtgg
     4141 ccgcgcggct cgctgctggt ggccgacgcc gccgcctacc tgcgcggcga gcgccgcttc
     4201 gaggcgctgt tcacgccgac ggccacgcgc tcgctcgccg gcttcggcgt gacgcgctcg
     4261 cgcgtgatgc tggacgtgct cgacgacgtc gcaggccgca tcgtcgagct gagcccgggg
     4321 cctgacggct ggacgcggcg cgaggtcgcg gcgcccttcc ccggcgcgct gtcgacgacc
     4381 gagctgcacg acccgacgct cgccgaggac ccgcttgccg agagctacct gctcggctac
     4441 accgacttcc tgacgcccga ctcgctctac ctcgccgagg cgggcagcga cgcgcgcgag
     4501 ctgctcaagg cgcggccgtc ctacttcgac ggcgccggca tgcgcgtcga gcagcgcttc
     4561 gcgcgctcgg ccgacggcac gcgcgtgccc tacttcgtcg tctggccggc cggcgcgacg
     4621 gccgacggcg acaacccgac gctgctgtac ggctacggcg gcttcgaggt ctcgctgacg
     4681 ccgtggtact ccggcgcgtt cggccgcgcc tggttcgcgc gcggcggcgt gctcgccgtc
     4741 gccaacatcc gcggcggcgg cgaatacggc cccgcgtggc accaggccgc ggtcaaggag
     4801 cacaagcagc gcagctacga cgacttcatc gcggtggcgg aggatctcgt cgctgccggc
     4861 atcacgaagc ccgcgcgcct gggcatcgag ggcggcagca acggcggcct gctggtcggc
     4921 gccgtgatgg tgcagcggcc cgagctgttc ggcgccgtga gctgccaggt gccgctgctg
     4981 gacatgcggc gcttcaacaa gctgctcgcc ggcgcgtcgt ggatggccga gtacggcaac
     5041 ccggacgccc tggacgactg ggcctggatc tcgcgctaca gcccgtacca gaacgtgcac
     5101 gccggccgcc gctacccgag cgtgctcttc acgacgtcca cgcgcgacga ccgcgtccac
     5161 cccggccacg cgcgcaagat ggccgcgcgc atgctgcagc agggccacga cgtcctctac
     5221 tacgagaaca tcgagggcgg ccacggcggc gccgcggaca acgcccagcg cgccgacctg
     5281 caggcgctgc agttcgcgtt cctctggcgc aagctgggca tgaagcgctg aaccgaaccc
     5341 ccggcgcgcg catggccggg catcggcctt gccgcgtcgc gggccatgaa ccttcgaaca
     5401 tccaccgacg cccccatgac ctacgagatc cgccacgacc cggcccgcca gcgcttcgag
     5461 accacggtcg acgggcagct gtgcgtggcc gactaccagc tgcgcggcga caccgtctgg
     5521 atgacgcaca ccggcgtgcc gcaggccgtc gaggggcgcg gcatcgccgc cgcgctcgtg
     5581 cgcaccgcgc tcgactggtg cgcggcgcag ggcttccggg tcgagccctc gtgcagctac
     5641 gtggccgtct acatgcgccg ccacccggag acgcagcgcc tgctggcgcg ctgagggcgg
     5701 ctaccgcgcg aatccgccga ggtagcggcc gagccacgac gccgcgagcg ccccggacag
     5761 ctcgccgcgc tcgaactcga agccgaacga gaccgcgtgc cgggaccgcg cgtgctcgcc
     5821 gcccggaatc gagcacgagg cgcgcgtgga cgtcgccgcg gctctgcggg gacgcccggc
     5881 gaggttctca ggcgcgcgac gacgcggcgg ccggcacgcg cgcggcctcg tcccggcgca
     5941 cgccccagcg ctgcgccagc acggcgcaca ccatcagctg cagctggtgg aacagcatca
     6001 gcggcaacac cagcgcgccg accgcatgcg cggcgaagag caccttggcc atggggatgc
     6061 cgctggccag gctcttcttc gagccgcaga agacgatcgt gatctggtcg gcgcggtcga
     6121 accccagcgc gcgcgcgatg cggccggtcg cgaacagcac cagtgccagc aacacgcagc
     6181 acacgacgaa gagccccacc agcgactgcc acggcacctg cttccagagg ccctcgatga
     6241 cggcggcgct gaaggccgtg tagaccacga gcaggatcga gccgcggtcg acgatgccga
     6301 cgatctcctt gcgccgcgcg atgacgccgg cgatccacgg ccgcgcgagg tggccggcca
     6361 ggaag
//
LOCUS       DASVLN010000462         5269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_132053, whole genome shotgun sequence.
ACCESSION   DASVLN010000462 DASVLN010000000
VERSION     DASVLN010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132053"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..709
                     /locus_tag="VF453_11370"
     CDS             179..709
                     /locus_tag="VF453_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEX7688301.1"
                     /translation="MSKKHPAGKKAASATPATSSAIDIGISAEDRAAISEGLARLLAD
                     TYTLYLTTHNFHWNVTGPMFNTLHAMFMGQYTELWNAVDPIAERIRSLGHHAPGSYGQ
                     FGRLSSLPDAPDQPPKALDMVRTLVAGHEAVARTARALFPLADKAGDEPTADLLTQRL
                     TVHEQTAWMLRSLLED"
     gene            740..1354
                     /locus_tag="VF453_11375"
     CDS             740..1354
                     /locus_tag="VF453_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX7688302.1"
                     /translation="MRVAIVTFDGFNELDSFIALGLINRLRPLGVQAEIACPSAQVTS
                     MNGVTVTAQQPLEFVLEADAVLFGSGIYTRAIAENAQLMDRLSLDPTRQLIGAQCSGT
                     LLLARLGLLGDMPACTDLTTKPWVIEAGVRVADTALLARGPVATAGGCMSSAYLAAWV
                     MWRAAGREATRQALHYAAPVGEKDAWVDHVLKVVGGALGESAAA"
     gene            1522..2355
                     /locus_tag="VF453_11380"
     CDS             1522..2355
                     /locus_tag="VF453_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX7688303.1"
                     /translation="MAQSPAHAFRPVARVALACTLAAAASLLVAGCGKKKTELGEGGS
                     VVTGSAGPAGAHNASHELLRCDAPVATMALVENPNGYAIQGGYNLPSSPLPLVRLIAQ
                     QSGCFRVVDRSAGLHATVQEQELQDRGIIRKNSTVHKGNGYEAQYTITPSLTFSEQNA
                     GHEVGGILGMIPVLNKFAGLADNVKLKEAQVALLLTDNETTEQVAAATGSVRVTDLGM
                     AGLMLGRAGGAAGAGWSNTNEGKVIAAAFLDAHNQLVTQLRMLQAKELPPPIPTKARA
                     G"
     gene            complement(2399..2680)
                     /locus_tag="VF453_11385"
     CDS             complement(2399..2680)
                     /locus_tag="VF453_11385"
                     /inference="COORDINATES: protein motif:HMM:NF045463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688304.1"
                     /translation="MTTYLYAKWKTAPADSPGEFYSELDPARWETRKVEVFPDGRMGY
                     ASAARSSNNGTRLSIIPLPPIEEIARQIEFDVRQASADEFDAAWKRATA"
     gene            2858..4486
                     /locus_tag="VF453_11390"
     CDS             2858..4486
                     /locus_tag="VF453_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX7688305.1"
                     /translation="MSADLAQQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGS
                     VKARKAARHATSDWMEIEKQRGISVASSVMQMEYRDCVINLLDTPGHQDFSEDTYRVL
                     TAVDAALMVIDAANGVEPQTRRLLQVCRARNTPILTFVNKMDREVQEPLALMDEIERE
                     LGMSVVPFTWPVGMGKAFGGVVDLRKRQMRVFAPGEDRVGGDEEVISDLAAPANAGRF
                     GMAWEQSQGEIELLREAAPVFEEAQFLEGHQTPMFFGSAINNFGVQEVLDALVELAPP
                     PGPRPAMQRAVEPAEPKFTGVVFKIQANMDPSHRDRIAFLRVASGHFERGMRLKVVRS
                     GKELRPNTVVSFLSQRRELLDEAFAGDIIGIPNHGVLQLGDTLTEGEQLQFTGLPFFA
                     PELFRSVEVADPLKTKQLRAGLTQLGEEGAIQVFRPVLGTVLLLGAVGQLQFEVVAHR
                     LEHEYGVKARITPARYALARWVTCDPADGGDNELKRFIDANAHRVAYDAVDAPAVLVE
                     YAPELRAIEANWPKIKFHALREHAGLVFQKHIGD"
     gene            4519..>5269
                     /locus_tag="VF453_11395"
     CDS             4519..>5269
                     /locus_tag="VF453_11395"
                     /inference="COORDINATES: protein motif:HMM:NF023426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEX7688306.1"
                     /translation="MRVLFIHQNLPGQFTHLIGALCGDPAHTAWGICAEPAAERARRL
                     HPKLDFVSYRPPTPPAAGLATSARELDLQVRRGQLVASILRQMGERGIAFDLVVAHPA
                     WGEALFVKDVLPDVPLLLYPEFFYAAEGADVGFDPEHPRTPQDAQRLRIRNLVPLSAL
                     EACDAAVVPTAWQHARLPAFARAKTRVVHEGVDTALARPDAAARFEVDGRVFAAGDPV
                     VTFVARNLEPYRGFHVFMRALPALLAAEPDAH"
BASE COUNT          765 a         1938 c         1814 g          752 t
ORIGIN      
        1 gattacgccg gcgcgggcag cctgctcgac gcccacgcct gaccgcgctc gccgcgtccg
       61 tccagggcgc gccgacccgg ccgcgcccca cccgactgcc ggatggggtc tttcgcctgc
      121 gccgttggcc ctttcggctc cgttgcctaa gcttcggcgt tccaccacga gggaacgcat
      181 gtccaagaag catccggccg gcaagaaggc cgcctccgcc accccggcca cctcgagcgc
      241 gatcgacatc ggcatctccg ccgaggaccg cgccgccatc agcgagggcc tcgcgcgcct
      301 gctcgccgat acctacaccc tgtatctgac gacccacaac ttccactgga acgtcacggg
      361 cccgatgttc aacacgctgc acgcgatgtt catgggccag tacaccgagt tgtggaacgc
      421 ggtcgacccg atcgccgagc gcatccgctc gctcggccac catgcgcccg gctcctacgg
      481 ccagttcggc cgcctgagct cgctgccgga cgcgcccgac cagccgccca aggcgctcga
      541 catggtgcgc acgctggtgg ccggccacga ggccgtcgcc cgcacggcgc gcgcgctgtt
      601 cccgctggcc gacaaggccg gcgacgaacc caccgccgac ctgctgaccc agcgcctgac
      661 cgtgcacgag cagacggcgt ggatgctgcg ctcgctgctc gaggactgag gcccgcgaac
      721 gggacccccg gagccgccga tgcgcgtcgc catcgtcacc ttcgacggct tcaacgagct
      781 ggactccttc atcgcgctgg gcctgatcaa ccgcctgcgg ccgctgggcg tccaggcgga
      841 gatcgcctgc ccctccgcgc aggtcacgtc gatgaacggc gtcacggtga cggcccagca
      901 gccgctggaa ttcgtgctgg aggccgacgc ggtgctgttc ggcagcggca tctacacgcg
      961 ggcgatcgcc gagaacgcgc agctcatgga tcgactgtcg ctcgatccga cgcgccagct
     1021 gatcggcgcg cagtgctccg ggacgctgct gctggcgcgg ctgggcctgc tgggcgacat
     1081 gccggcgtgc acggacctca cgaccaagcc ctgggtgatc gaggcgggcg tgcgcgtggc
     1141 cgacacggct ctcctcgcgc gcgggccggt cgcgacggcg ggcggctgca tgtcgtccgc
     1201 ctacctcgcc gcctgggtga tgtggcgcgc cgccggccgg gaggccacgc gccaggcgct
     1261 gcactacgcg gcaccggtgg gcgagaagga cgcgtgggtc gaccacgtgc tgaaggtcgt
     1321 cggcggcgcg ttgggggagt ccgcggcggc ctgaacgccg agcggtcggc gcgactaccc
     1381 cgacaggggg gattgcggcg cccggcagcc attgttccaa tccgcgttcg cgcgcggcga
     1441 tcacctgcgg tgacgcgccg gcgccgcggg cgctggcatg atcgcgcccg atcacgacaa
     1501 ccacctggag aggttcgaac gatggcgcaa tcccccgcgc atgccttccg tcccgtcgcg
     1561 cgcgtcgcgc tcgcctgcac gctcgccgcc gcggcgtcgc tgctggtggc cggctgcggc
     1621 aagaagaaga ccgagctcgg cgagggtggc tccgtcgtca ccggctcggc gggcccggct
     1681 ggggcgcaca acgcgtccca cgagctgctg cgctgcgatg ccccggtggc gacgatggcg
     1741 ctggtcgaga accccaacgg ctacgcgatc cagggcggct acaacctgcc ctcctcgccg
     1801 ctgccgctgg tgcggctgat cgcgcagcag agcggctgct tccgcgtcgt cgaccgctcc
     1861 gccggcctgc acgccacggt gcaggagcag gagctgcagg accgcggcat catccgcaag
     1921 aactccaccg tccacaaggg caacggctac gaggcgcagt acacgatcac gcccagcctg
     1981 accttcagcg aacagaacgc cggccacgag gtcggcggca tcctcggcat gatcccggtg
     2041 ctgaacaagt tcgccggcct ggccgacaac gtcaagctga aggaggcgca ggtcgccctg
     2101 ctgctgaccg acaacgagac gaccgagcag gtcgccgccg cgacgggctc ggtgcgcgtc
     2161 accgacctcg gcatggccgg cctgatgctg gggcgcgcgg gcggcgcggc gggcgcgggc
     2221 tggagcaaca cgaacgaggg caaggtgatc gccgcggcct tcctcgacgc ccacaaccag
     2281 ctcgtgacgc agctgcgcat gctgcaggcc aaggagctgc cgccgccgat cccgacgaag
     2341 gcgcgggcag gctgagcaag ctcagggcgg agcgaggtcg tacacggcca cgtcggcttc
     2401 acgccgtcgc gcgcttccag gcggcgtcga actcgtccgc ggacgcttgc ctgacgtcga
     2461 attcgatctg gcgggcgatc tcctcgatgg gaggcaacgg aatgatcgac agtctcgtcc
     2521 cgttgttcga ggaccttgcg gccgacgcat agcccatgcg tccatccggg aacacctcga
     2581 ccttgcgcgt ctcccagcgc gcgggatcca gttcgctgta gaactcaccc gggctgtcgg
     2641 ccggagcggt cttccatttt gcgtacaggt aggtcgtcat cgtcttcttt cactccttgc
     2701 cgttcgccat cagggcgcct cgaagtgtcc ttcccggctg cgcaaacggg caggcctcgt
     2761 cgaggcgcct cggcatcggg cgccggcacg cgataatcgc gggttcaccc ccttccagcg
     2821 tcgcgagcct gccggcacga cggcgcacga gcccgccatg tccgccgacc tcgcccagca
     2881 ggtgcgccgc cgccgcacct tcgccatcat ctcccacccc gacgcgggca agaccacgct
     2941 caccgagaag ctgctgctgt tctccggcgc gatccagatc gccggctcgg tgaaggcgcg
     3001 caaggcggcg cggcacgcga cttccgactg gatggagatc gagaagcagc gcggcatctc
     3061 ggtggcgtcc tcggtgatgc agatggagta ccgcgactgc gtcatcaacc tgctggacac
     3121 cccgggccac caggacttct cggaagacac ctaccgcgtg ctgaccgccg tcgacgccgc
     3181 gctgatggtg atcgacgcgg ccaacggcgt cgagccgcag acgcgccgcc tgctgcaggt
     3241 ctgccgcgcg cgcaacacgc ccatcctgac cttcgtcaac aagatggacc gcgaggtgca
     3301 ggagccgctc gcgctgatgg acgagatcga gcgcgagctg ggcatgagcg tcgtgccctt
     3361 cacgtggccg gtcggcatgg gcaaggcctt cggcggcgtg gtcgacctgc gcaagcgtca
     3421 gatgcgcgtg ttcgcgccgg gcgaggaccg ggtcggcggc gacgaggaag tcatcagcga
     3481 cctcgccgcg ccggccaacg ccgggcgctt cggcatggcc tgggagcagt cgcagggcga
     3541 gatcgagctg ctgcgcgagg ccgcgcccgt cttcgaggag gcgcagttcc tcgagggcca
     3601 ccagacgccg atgttcttcg gctcggcgat caacaacttc ggcgtgcagg aggtgctgga
     3661 cgcgctcgtc gagctcgctc cgccgcccgg cccgcgcccc gcgatgcagc gcgcggtcga
     3721 gccggcggag ccgaagttca ccggcgtggt cttcaagatc caggccaaca tggacccgtc
     3781 gcaccgcgac cgcatcgcgt tcctgcgcgt cgcctcgggc cacttcgagc gcggcatgcg
     3841 gctgaaggtc gtgcgctcgg gcaaggagct gcggcccaac acggtcgtct cgttcctgtc
     3901 gcagcgccgc gagctgctgg acgaggcctt cgccggcgac atcatcggca tcccgaacca
     3961 cggcgtgctg cagctgggcg acacgctcac cgagggcgag cagctgcagt tcacggggct
     4021 gccgttcttc gcgccggagc tgttccgcag cgtcgaggtg gccgatccgc tgaagaccaa
     4081 gcagctgcgc gcgggcctca cgcagctcgg cgaggaaggg gcgatccagg tcttccggcc
     4141 ggtgctggga accgtcctgc tgctgggcgc ggtcggccag ctgcagttcg aggtcgtggc
     4201 gcaccgcctc gagcacgagt acggcgtcaa ggcccgcatc acgccggcgc gctacgcgct
     4261 cgcgcgctgg gtcacctgcg acccggccga cggcggagac aacgagttga agcgcttcat
     4321 cgacgccaac gcccaccgcg tggcctacga cgccgtcgac gcgccggccg tgctggtcga
     4381 atacgcgccc gagctgcgcg cgatcgaggc caactggccg aagatcaagt tccacgcgtt
     4441 gcgcgagcac gccgggctcg tcttccagaa gcacatcggc gattgacggg ctcgcgcatc
     4501 gcccacgatg accgtccgat gcgcgtcctg ttcatccacc agaacctgcc ggggcagttc
     4561 acgcacctga tcggcgcgtt gtgcggcgat ccggctcata cggcatgggg tatctgcgcg
     4621 gagccggcgg ccgaacgcgc caggcggctg catccgaagc tggacttcgt gtcgtaccgc
     4681 ccgcccacgc cgcccgcggc cgggctggcc acgtcggcgc gcgagctcga cctccaggtg
     4741 cggcgcggcc agctggtcgc gagcatcctg cgccagatgg gcgagcgcgg gatcgcgttc
     4801 gacctcgtgg tcgcgcaccc ggcctggggc gaggccctgt tcgtcaagga tgtcctgccg
     4861 gacgtgccgc tgctgctcta ccccgagttc ttctacgccg ccgagggcgc cgacgtcggg
     4921 ttcgacccgg agcatccgcg cacgccgcag gatgcgcagc gcctgcggat tcgcaacctc
     4981 gtgccgctgt ccgcgctgga ggcctgcgac gccgccgtcg tcccgaccgc ctggcagcac
     5041 gcacgcctgc cggccttcgc gcgagccaag acccgcgtgg tccacgaggg cgtcgacacg
     5101 gcgctcgccc ggcccgatgc cgccgcgcgc ttcgaggtcg acgggcgcgt gttcgccgcg
     5161 ggcgacccgg tcgtgacctt cgtcgcgcgc aacctcgagc cctaccgcgg cttccacgtc
     5221 ttcatgcgtg ccctgcccgc gctgctggcc gccgagcccg acgctcacg
//
LOCUS       DASVLN010000463         3507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_78005, whole genome shotgun sequence.
ACCESSION   DASVLN010000463 DASVLN010000000
VERSION     DASVLN010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78005"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VF453_11400"
     CDS             complement(<1..205)
                     /locus_tag="VF453_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688307.1"
                     /translation="MSYGTILLHLGDTPGLEARIDAAIHLARRHGSHLVALAATGNAR
                     FETSLGAGLLPREQLREALAAARR"
     gene            378..1757
                     /locus_tag="VF453_11405"
     CDS             378..1757
                     /locus_tag="VF453_11405"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7688308.1"
                     /translation="MKLTFLGATGTVTGSRYLVEHGDRRVLVDCGLFQGLKQLRLRNW
                     APLPVDPSSIDAVVLTHAHIDHSGYLPRLVAQGFRGTVHATAPTSELCGLLLPDCGHL
                     QEEEAAYANRHKTSRHSPALPLYTRDEARAALKRFQLHDFATTFEPVPGAYVRFTSAG
                     HILGAASVHLLTERGSLLFSGDLGRGDDLLMKPPSPPPAADVVVVESTYGDRLHDRSD
                     TLAALADIVNRTAARGGHVIVPAFAVGRAQLLLHALRLLKDAHRIPDLPVHLDSPMAT
                     DATALYQRHPGLHRLDAAACAAMARDVRIVNTQEDSQALAALRYPAIIVSASGMATGG
                     RVLNHIEHFAPNPACTLLFVGYQAMGTRGAALVGGAREVRMHGRWVPVRAEVATLEGL
                     SSHADRDELLAWLGALPSPPRRVYVTHGEPAAADSLRQAISEKLQWHSAVPEYRDALD
                     VGALVDGDA"
     gene            complement(1800..2291)
                     /locus_tag="VF453_11410"
     CDS             complement(1800..2291)
                     /locus_tag="VF453_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7688309.1"
                     /translation="MSPATDRDRSPADPLAVGCLCTRILTVVRRETPLVDAARRMREE
                     HVGCVVVAEPTPTGDVAVGILTDRDIVVSVVALGIDPAALAVSDVMTAAVSSVPEGGS
                     IGTVLALMRDQGIRRVPVTSADGVLVGLLTFDDLVAALTLQTQAMTEALVVGRQREGD
                     VHP"
     gene            complement(2288..2791)
                     /locus_tag="VF453_11415"
     CDS             complement(2288..2791)
                     /locus_tag="VF453_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7688310.1"
                     /translation="MYDRILVPIDGSEASDAGLAEAIRLARLSGGTVRLLHVVEEVPL
                     LPAALFTHEPAARGLNAYQCGSAMVERRAARVRRAGVKAEVLLVEDGERRLQEHVARA
                     VDAWPADIIVLGSHGRRGASRVVLGSDAEQIARKSRVPVLLAHAAGPAPAIPDRSEPA
                     FASEDET"
     gene            complement(2817..3011)
                     /locus_tag="VF453_11420"
     CDS             complement(2817..3011)
                     /locus_tag="VF453_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX7688311.1"
                     /translation="MDINEGVVDRTIRIVAGAGLVGAAALGLLGWWAWIGVVPLVTGI
                     VGTCPLYSMLGVRTCAPQHR"
     gene            complement(3238..>3507)
                     /locus_tag="VF453_11425"
     CDS             complement(3238..>3507)
                     /locus_tag="VF453_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7688312.1"
                     /translation="CRPGGDEQACRPGLDRAAAWLSAHGVSVQARVSPSELPVGEALL
                     SEAAEVGAGLLVMGAWGHRRVVERLLGGATRTLVHSMTMPVLFAH"
BASE COUNT          469 a         1378 c         1229 g          431 t
ORIGIN      
        1 gccgcctggc cgcggccagc gcctcgcgca gctgctcgcg cggcagcagg ccggcgccga
       61 ggctggtctc gaagcgcgcg ttgccggtgg ccgccagggc caccagatgg ctgccgtgac
      121 ggcgcgccag gtggatcgcc gcgtcgatgc gcgcctccag gcccggcgtg tcgcccaggt
      181 gcagcaggat cgttccgtag ctcatgcggc ctccgtttcg cgcgcccggc agcgggtcat
      241 gcgggaagcc gatcgtgtcc cggcgcgcgg cagcgggctt gcgccggatc aaggcgaggc
      301 cgtcgccgcc atgacgagcg tcaagcgaag gtccccgccg caccctatcg tggcgcatca
      361 ccttgtggga gaccgcgatg aagctcacct tcctcggcgc gacgggcacc gtcacgggct
      421 cgcgctacct ggtggagcac ggcgaccgcc gcgtgctcgt cgactgcggg ctgttccagg
      481 gcctcaagca gctgcggctg cgcaactggg cgccgctgcc ggtcgacccc tcctcgatcg
      541 acgccgtggt gctgacccat gcgcacatcg accacagcgg ctacctgccg cgactcgtcg
      601 cgcagggctt ccgcggcacg gtccacgcga ccgcgccgac cagcgagctc tgcggcctgc
      661 tgctgccgga ctgcggccat ctccaggaag aggaggcggc ctacgcgaac cgccacaaga
      721 cgagccgcca ttcgcccgcc ctgccgctgt acacgcgcga cgaggcgcgg gcggcgctga
      781 agcgattcca gctccacgac ttcgccacca ccttcgagcc ggtgcccggc gcctatgtcc
      841 gcttcaccag cgcgggccac atcctcggcg cggccagcgt ccacctgctc acggagcgcg
      901 gcagcctgct gttctccggc gacctcggcc gcggcgacga cctgctgatg aagccgccgt
      961 ccccgcctcc cgcggccgac gtcgtggtcg tggaatcgac ctacggcgac cgcctgcacg
     1021 accgcagcga caccctggcg gcgctcgcgg acatcgtcaa ccgcaccgcc gcgcgcggcg
     1081 gccacgtgat cgtgccggcg ttcgcggtcg gacgggccca gttgctgctg cacgccctgc
     1141 gcctgctcaa ggacgcccat cgcatccccg acctgccggt gcacctcgac agccccatgg
     1201 cgaccgacgc cacggcgctc taccagcgtc atcccggcct gcaccgcctc gacgccgccg
     1261 cctgcgccgc gatggcgcgc gacgtgcgca tcgtcaacac gcaggaggac tcgcaggcgc
     1321 tcgcggcgct gcgctatccg gcgatcatcg tctccgcgag cggcatggcg accggcgggc
     1381 gggtcctgaa ccacatcgag catttcgcgc cgaacccggc ctgcaccctg ctgttcgtgg
     1441 gctaccaggc gatgggcacg cgcggcgccg cgctggtggg cggcgcgcgc gaggtgcgca
     1501 tgcacggccg ctgggtgccg gtgcgcgcgg aggtcgccac gctcgaaggc ctgtcgtcgc
     1561 acgccgaccg ggacgagctc ctcgcctggc tgggcgccct gcccagcccg ccgcgccgcg
     1621 tctatgtcac ccacggcgag cccgccgccg cggactcgct gcggcaggcg atctcggaga
     1681 agctgcaatg gcacagcgcc gtgccggagt accgcgacgc gctggacgtc ggcgcgctgg
     1741 tggacgggga cgcctgagcc tgtccgcgga cggggcgtgc aaccgcgccg gccccgcgtt
     1801 cacgggtgca cgtcgccctc gcgctgccgg ccgacgacca gcgcctccgt catggcctgc
     1861 gtctgcagcg tcagggcggc gacgaggtcg tcgaaggtca gcagcccgac cagcaccccg
     1921 tcggcggacg tgacgggcac gcgccggatg ccctggtccc gcatcagcgc gagcaccgtg
     1981 ccgatcgagc cgccctcggg cacgctcgat acggcggccg tcatcacgtc cgacaccgcc
     2041 agggcggccg gatcgatgcc cagcgcgacg accgacacga cgatgtcgcg atcggtgagg
     2101 atgccgacgg cgacgtcgcc cgtgggcgtc ggttcggcga cgacgacgca gccgacgtgc
     2161 tcctcgcgca tgcgccgcgc cgcgtcgacc agtggcgtct cccggcgcac gacggtcagg
     2221 atgcgcgtgc acaggcagcc gaccgccagc ggatcggccg gcgagcgatc gcgatcggtc
     2281 gccgggctca cgtctcgtcc tccgaggcga aggcgggctc gctgcgatcc gggatggccg
     2341 gcgccggacc ggccgcgtgc gccagcagca ccggcacgcg gctcttgcgg gcgatctgct
     2401 cggcatcgct gcccagcacc acgcggctcg cgccgcgccg gccgtggctg cccagcacga
     2461 tgatgtcggc cggccacgcg tcgaccgcgc gcgccacgtg ctcctgcagc cggcgctcgc
     2521 cgtcctccac gagcaggacc tccgccttga cgccggcacg gcggacgcgc gccgcacggc
     2581 gctcgaccat cgcgctgccg cactggtagg cgttcaggcc gcgagcggcc ggctcgtgcg
     2641 tgaagagcgc cgccggcagc agcggcacct cctcgaccac gtgcagcagc ctcaccgtgc
     2701 cgccgctcag gcgcgccagg cggatcgcct cggccagccc ggcgtcggac gcttcgctgc
     2761 cgtcgatcgg cacgaggatg cggtcgtaca tgcgacgacg ccccgtcgat ccggtgctag
     2821 cgatgctgcg gcgcgcaggt gcgcacgccg agcatcgagt acagcggaca ggtgcccacg
     2881 atgccggtga ccagcgggac gacgccgatc caggcccacc agcccagcag gccgagggcc
     2941 gccgccccga cgaggccggc accggccacg atgcggatcg tgcgatccac gacgccttcg
     3001 ttgatgtcca tgatgatcct cgcgttcacc tggcgcggcg ccatctgccg cggctccaag
     3061 atgccagggc ccgcggcgtc cggattgacg gcgatcaagg gctcgcgggc ggcggctcag
     3121 tcgcggcgtg cgagcaccca cgcgaccgcc cacgccacca gcgcggtcgc cgcgaccacg
     3181 cccacggcca ggaggatcca tccgagcatg cgtcgtctcc cctgccgcgg tgccgcctca
     3241 atgggcgaag agcaccggca tcgtcatgct gtggacgagg gtgcgcgtcg cgccgccaag
     3301 cagtcgttcg accacgcgcc gatgccccca ggcgcccatc acgagcaggc cggcgccgac
     3361 ctcggccgcc tccgacagca acgcttcgcc gaccggcagc tcgctgggcg agacccgggc
     3421 ctgcaccgac acgccgtgcg ccgacagcca tgcggccgcg cggtccagcc cgggccggca
     3481 cgcctgctcg tcgccgcccg gacggca
//
LOCUS       DASVLN010000464         6836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_237998, whole genome shotgun sequence.
ACCESSION   DASVLN010000464 DASVLN010000000
VERSION     DASVLN010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237998"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF453_11430"
     CDS             <1..451
                     /locus_tag="VF453_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7688313.1"
                     /translation="LMGAVADAALEAGAGVLGVIPRRLVARELGHRGIHELRIVDTMH
                     DRKLQMAREASAFVAMAGGIGTLEELFETWTWQQLGYHARPIGLLDTAGFYAPLQALL
                     AHTREAGFVEAETVARLRIDAEPGRLLDRLHDEAVLVGPDGADFNPV"
     gene            complement(488..826)
                     /locus_tag="VF453_11435"
     CDS             complement(488..826)
                     /locus_tag="VF453_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7688314.1"
                     /translation="MKQITAVIKPFKLEEVREALGDVGVSGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVKDSDVERCIEAVVKAAKTGKIGDGKIFVTAVEQVVRI
                     RTGETDESAV"
     gene            complement(873..2624)
                     /locus_tag="VF453_11440"
     CDS             complement(873..2624)
                     /locus_tag="VF453_11440"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX7688315.1"
                     /translation="MPDREPVRVALLQINPTVGDLDGNARVIADAARAAWTQGARVAL
                     APELALCGYLPEDLLLRPAFLAACAQALERLAAAIADCEGLQVVVGHPWDGGAAPRPA
                     AGVEDDGHGGHVRSKSVSVPSVFNAASVLAGGRVAGTYCKRELPNYQVFDERRYFVSG
                     RDAGFGPLVVESGGLRLGVLICEDAWFEEPAMLATGAGADVLCVLNASPFHLGKPAER
                     EERMARCARANARPLLYAHMTGGQDEMVFDGGSFVLDAAGAVVARAPLFASATLAVDL
                     APGAAPVPAAGSALEPLPELHAQAWAALVTGVRDYIGKNGFPSAIIGLSGGIDSALVL
                     AIAVDALGADRVRAVMMPSRYTADISWIDARDMAARLGVRYDEIAIAPMFDAFRASLA
                     DQFAGLPEDATEENVQARVRGTLLMALSNKTGAIVLTTGNKSEMATGYCTLYGDMAGG
                     FAVIKDVAKTLVYALAEWKNRQPTPRADGTTGPVIPERIITRPPSAELRPDQTDQDSL
                     PPYEVLDAILAAYMEEDRPIEEIVAAGHARADVERVTRLIKINEYKRRQSPVGIRITH
                     RGFGRDWRYPITSRFRA"
     gene            2651..3904
                     /locus_tag="VF453_11445"
     CDS             2651..3904
                     /locus_tag="VF453_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7688316.1"
                     /translation="MRAGDYLIEVHEGVGAIEASEWDALVSAQACATPFLRHAYLRAL
                     EDSGSVGDDTGWTPVALTVRERRGGRALVAAAPAYLKAHSYGEYVFDWAWADAYHRHG
                     LDYYPKLVVAVPFTPVPGSRLLARGPEARALLLQALGTLAREADASSVHVLFDDEADD
                     SAARAAGWMTRHGVQFHWHNRGQAPGEDAGAPPYADFGDFLASLQRDKRKKIAQERRR
                     VAEAGVTFQTLEGAAIDEAAWDFFHECYCNTYAAHHSTPYLTREFFARMQATMPEHWV
                     MFAASRGGRRIACSLVAVDPAAGVAYGRYWGALEAIPNVHFEACYYQPLAWCIANGFR
                     RFEGGAQGEHKMARGLIPVRTQSAHWVVEPAFARAIGAHLEHEAGAMATYLDELDDRS
                     PFRRAPPGSNAPPANEPPDASPGRA"
     gene            complement(3960..4502)
                     /gene="ppa"
                     /locus_tag="VF453_11450"
     CDS             complement(3960..4502)
                     /gene="ppa"
                     /locus_tag="VF453_11450"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX7688317.1"
                     /translation="MSLHLVNPGAKAPEEFNVIIEIPMNADPIKYEVDKESGALFVDR
                     FMTTAMHYPCNYGYVPKTLSDDGDPVDVLVITPFPLTPGVVVTCRAIGVLQMDDEAGG
                     DAKLLAVPTTKILPIYEHWKKPEDINQMRLKAIQHFFEHYKDLEPNKWVKVKGWAGPE
                     EAKAEITNGIAAYKKAHGES"
     gene            4708..>6836
                     /locus_tag="VF453_11455"
     CDS             4708..>6836
                     /locus_tag="VF453_11455"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX7688318.1"
                     /translation="MSSNLALEMALACGLVAVLYGFIQRSWILRQDAGNPRMQEIAAA
                     IQQGAAAYLSRQYRTIALVGVVLAVIIAWLLGPMTAGGYVLGAVLSGACGFIGMNVSV
                     RANVRTAQAATGGIAPALDVAFKGGAITGMLVVGLGLLGVGLFYWFISGGHAADPATL
                     KPLLGFAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYAVTLIATMTLGALTMTAMAGSAVVYPLALGGVSIVASIIGC
                     FFVKASAGMKNVMPALYKGLAVAGILSLVAFFFVTKSLVPDDALQGGNTVMRLFGACV
                     VGLVLTGAMVWITEFYTGTQYGPVQHVAKASTTGHGTNIIAGLGVSMKSTAWPVLFVC
                     VAIFIAYKLAGLYGIAVGATSMLSMAGIVVALDAYGPITDNAGGIAEMAELPESVRDV
                     TDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHGLEGRGMHVDFELADFRVIIGL
                     FIGGMIPYLFGAMAMEAVGRAAGSVVEEVRRQFRDIAGIMEGTGKPEYGRAVDMLTSA
                     AIKEMVVPSLLPVIAPVLVGMLLGPAALGGLLMGTIVTGLFVAISMCTGGGAWDNAKK
                     YIEDGNFGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPLIKIINIVALLIVPLLPAAG
                     GAVDAATDRQIAPTPIAAPLPAQEASPP"
BASE COUNT          945 a         2414 c         2441 g         1036 t
ORIGIN      
        1 cctgatgggc gcggtcgccg acgccgcgct ggaggccggg gccggcgtgc tcggcgtgat
       61 cccgcggcga ctcgtcgcgc gcgagctggg acatcgcggc atccacgagc tgcgcatcgt
      121 cgacaccatg cacgaccgca agctgcagat ggcgcgcgag gccagtgcct tcgtcgcgat
      181 ggccggcggc atcggcacgc tggaggagct gttcgagacc tggacctggc agcagctcgg
      241 ctaccacgcg cgcccgatcg ggctgctgga caccgccggc ttctacgcgc cgctgcaggc
      301 gctgctcgcg cacacgcgcg aggccggctt cgtcgaggcg gagacagtcg cgcggctgcg
      361 catcgacgcg gagcccggcc gcttgctgga tcgcctgcac gacgaggcgg tgctcgtcgg
      421 ccccgacggc gcggacttca acccggtgtg aggcggcggc cgcgcgccgt cccgcgagcg
      481 ccaggcgtca gaccgccgat tcgtcggtct cgccggtgcg gatgcgcacg acctgctcga
      541 ccgccgtcac gaagatcttg ccgtcgccga tcttgccggt cttggcggcc ttgacgaccg
      601 cctcgatgca gcgctcgacg tccgaatcct tgaccacgac ctcgatcttg accttgggca
      661 ggaagtcgac cacgtactcg gcgccgcggt agagctccgt gtgccccttc tggcggccga
      721 agcccttgac ctcggtcacc gtgagcccgg agacgcccac gtcgccgagc gcctcgcgca
      781 cttcctcgag cttgaacggc ttgatgacgg cggtgatctg tttcatggcg ggctccggtt
      841 ggacgtgggg atgctgcggg atcggggtcg atttaagcac ggaagcgcga ggtgatcgga
      901 taccgccagt cgcgcccgaa gccgcggtgg gtgatgcgga tgccgaccgg cgactgccgg
      961 cgcttgtact cgttgatctt gatgaggcgc gtgacgcgct cgacgtccgc gcgggcgtgg
     1021 ccggcggcga cgatctcctc gatcggccga tcctcctcca tgtacgcggc gaggatcgcg
     1081 tcgagcacct cgtagggcgg caggctgtcc tggtcggtct ggtcggggcg cagctcggcc
     1141 gagggcggcc gggtgatgat gcgctcgggg atgaccgggc cggtcgtgcc gtcggcgcgc
     1201 ggcgtcggct ggcggttctt ccattcggcc agcgcgtaga ccagcgtctt ggcgacgtcc
     1261 ttgatgaccg cgaagccacc ggccatgtcg ccgtagagcg tgcagtagcc ggtggccatc
     1321 tcgctcttgt tgccggtggt cagcacgatg gcgccggtct tgttggacag cgccatcagc
     1381 agcgtgccgc gcacgcgggc ctggacgttc tcctcggtcg catcctcggg caggccggcg
     1441 aactggtccg cgagcgaggc gcggaaggcg tcgaacatcg gggcgatcgc gatctcgtcg
     1501 tagcgcacgc ccagccgtgc ggccatgtcg cgggcatcga tccaggagat gtcggcggtg
     1561 tagcgtgagg gcatcatgac ggcgcgcacg cgatcggcgc cgagcgcgtc gaccgcgatc
     1621 gcgagcacca gcgccgagtc gatgccgccc gacagcccga tgatggcgct cgggaagccg
     1681 ttcttgccga tgtagtcgcg cacgccggtc acgagcgcgg cccaggcctg cgcgtgcagc
     1741 tcgggcagcg gctcgagcgc cgatcccgca gccggcaccg gcgcggcgcc gggcgcgagg
     1801 tcgaccgcca gcgtcgccga tgcgaacaac ggcgcgcggg cgacgaccgc cccggctgcg
     1861 tcgagcacga acgagccgcc gtcgaagacc atctcgtcct ggccgccggt catgtgcgcg
     1921 tacagcagcg gccgcgcgtt ggcgcgcgcg cagcgggcca tgcgctcctc gcgctcggcc
     1981 ggcttgccga ggtggaacgg cgaggcgttg agcacgcaca gcacgtccgc gcccgcgccg
     2041 gtggccagca tcgcgggctc ctcgaaccag gcgtcctcgc agatcagcac gcccagccgc
     2101 aggccgcccg actccacgac cagcggcccg aagccggcat cgcgccccga gacgaagtag
     2161 cgccgctcgt cgaacacctg gtagttgggc agctcgcgct tgcagtaggt gccggcgacg
     2221 cgtccccccg cgagcaccga ggccgcgttg aagacgctgg gcacgctcac ggacttcgac
     2281 ctaacatggc cgccgtgccc gtcgtcctcc acccccgccg ccggccgcgg cgccgcgccg
     2341 ccgtcccacg gatggcccac caccacttgc aggccctcgc agtcggcgat cgccgcggcg
     2401 agccgctcga gcgcctgcgc gcaggccgcg aggaaggccg gccgcagcag caggtcctcg
     2461 ggcagatagc cgcacagcgc gagctcgggc gccagcgcca cgcgcgcgcc ctgcgtccat
     2521 gcggcgcggg cggcatcggc gatcacacgg gcgttgccgt cgaggtcgcc gacggtcggg
     2581 ttgatctgca gcagtgcgac ccgcacgggt tctcgatcag gcatggagtc tggacagtcg
     2641 gggcgaggcg gtgagagcgg gcgattatct catcgaggtc cacgagggcg tgggcgcgat
     2701 cgaggcgtcc gagtgggacg cgctcgtgtc cgcgcaggcc tgcgcgacgc ccttcctgcg
     2761 ccacgcctac ctgcgtgcgc tggaggactc cggcagcgtg ggcgacgaca ccggctggac
     2821 gcccgtcgcg ctgaccgtgc gcgagcgccg tggcggccgc gcgctggtcg ccgccgcgcc
     2881 ggcctacctg aaggcgcaca gctacggcga gtacgtgttc gactgggcct gggccgacgc
     2941 ctaccaccgc cacggcctcg actactaccc gaagctggtc gtggccgtgc ccttcacgcc
     3001 ggtgcccggc tcgcgcctgc tcgcgcgagg ccccgaggca cgcgccctgc tgctgcaggc
     3061 gctcggcacc ctcgcgcgcg aggcggacgc gtcgtcggtg cacgtgctgt tcgacgacga
     3121 ggccgatgac tccgccgccc gcgcggccgg ctggatgacg cggcacggcg tgcagttcca
     3181 ctggcacaac cgcggccagg cgcccggcga ggacgcgggt gccccgccct atgccgactt
     3241 cggcgacttc ctcgccagcc tgcagcgcga caagcgcaag aagatcgccc aggaacggcg
     3301 ccgcgtcgcc gaggccgggg tgacgttcca gacgctggaa ggcgccgcga tcgacgaggc
     3361 cgcctgggac ttcttccacg agtgctactg caacacctac gcggcgcacc actcgacgcc
     3421 atacctcacg cgggagttct tcgcccgcat gcaggcgacg atgccggagc actgggtgat
     3481 gttcgcggcc tcgcgcggcg ggcggcggat cgcgtgctcg ctggtcgcgg tcgaccccgc
     3541 ggcgggcgtc gcctacggcc gctactgggg cgcgctcgag gcgatcccca acgtccactt
     3601 cgaggcctgc tactaccagc cgctcgcgtg gtgcatcgcc aacgggttcc ggcgcttcga
     3661 gggcggcgcg cagggcgagc acaagatggc gcgcggcctg atcccggtgc gcacgcagtc
     3721 ggcgcactgg gtcgtcgagc cggccttcgc gcgcgcgatc ggcgcccacc tcgagcacga
     3781 ggccggcgcg atggcgacct acctcgacga gctggacgac cgctcgccgt tccgccgcgc
     3841 tccccccggc tccaacgcgc cgcccgcgaa cgaaccgccg gacgcgtcgc ccgggcgcgc
     3901 gtgacgcgca tgacaaaggg ccgcacgcgg cggcccttcc tcgtcggcgc ggcggcccct
     3961 cagctctcgc cgtgcgcctt cttgtacgcc gcgatgccgt tggtgatctc ggccttggct
     4021 tcctcggggc cggcccagcc cttgaccttg acccacttgt tcggctcgag atccttgtag
     4081 tgctcgaaga agtgctggat cgccttcagg cgcatctggt tgatgtcctc gggcttcttc
     4141 caatgctcgt agatcggcag gatcttggtc gtcggcacgg ccagcagctt ggcgtcgccg
     4201 cccgcttcgt cgtccatctg cagcacgccg atcgcgcggc aggtcacgac gacgcccggc
     4261 gtcagcggga acggcgtgat gaccagcacg tcgaccgggt cgccgtcgtc ggacagggtc
     4321 ttcgggacgt agccgtagtt gcaggggtag tgcatcgcgg tcgtcatgaa gcggtcgacg
     4381 aacagcgcgc cggactcctt gtcgacctcg tacttgatcg gatccgcgtt catcgggatc
     4441 tcgatgatga cgttgaattc ctcgggcgcc ttggcgccgg ggttgacgag atgcaggctc
     4501 atggtgtcga gtgggctgag gggtcgtttc tcgggactaa ccctgcattc taaggggcca
     4561 gggaaacccg aggtaaacca gcatgacgac gcggtgagcc agccgctagg atggattcgc
     4621 gacctcgcgg gtccgtgaat gaagcagttg taggccgaca gatgtgaggc gcatccgatc
     4681 cacacgacgg gaaggagaca actcttgatg tcatcgaact tggcgctgga gatggcgctc
     4741 gcgtgcggcc tggtggccgt gctctacggg ttcatccagc gcagctggat cctgaggcag
     4801 gacgcgggca acccccgcat gcaggagatc gcggcggcca tccagcaggg cgcggccgcc
     4861 tacctgtccc gccagtaccg cacgatcgcg ctcgtgggcg tcgtgctggc ggtgatcatc
     4921 gcctggctgc tcggcccgat gacggccggc ggctacgtgc tcggggccgt gctgtccggc
     4981 gcctgcggct tcatcggcat gaacgtctcc gtgcgcgcca acgtccgcac cgcgcaggcg
     5041 gccacgggcg gcatcgcgcc ggccctggac gtcgcgttca agggcggcgc gatcaccggc
     5101 atgctcgtgg tgggcctcgg cctgctgggg gtcggcctgt tctactggtt catcagcggc
     5161 ggccacgccg ccgacccggc cacgctcaag ccgctgctcg gcttcgcgtt cggctcgtcg
     5221 ctgatctcga tcttcgcgcg cctgggcggc ggcatcttca ccaagggcgc ggacgtcggc
     5281 gccgacctgg tgggcaaggt cgaggcgggc atccccgagg acgacccgcg caacccggcc
     5341 gtgatcgccg acaacgtggg cgacaacgtc ggcgactgcg ccggcatggc cgccgacctg
     5401 ttcgagacct acgccgtcac gctcatcgcc acgatgacgc tgggcgccct caccatgacg
     5461 gcgatggccg gctcggccgt ggtctacccg ctcgcgctgg gcggcgtgtc catcgtcgcg
     5521 tccatcatcg gctgcttctt cgtcaaggca tcggcgggca tgaagaacgt gatgccggcg
     5581 ctgtacaagg gcctggcggt cgcgggcatc ctgtcgctgg tggcgttctt cttcgtcacg
     5641 aagtcgctcg tgcccgacga cgcgctgcaa ggcggcaaca ccgtgatgcg gctgttcggg
     5701 gcgtgcgtcg tgggcctcgt gctgaccggc gcgatggtgt ggatcaccga gttctataca
     5761 ggtacccagt atggcccggt gcagcacgtc gccaaggcct cgacgaccgg ccacggcacc
     5821 aacatcatcg ctggcctcgg cgtgtcgatg aagtccaccg cgtggccggt gctgttcgtc
     5881 tgcgtcgcga tcttcatcgc ctacaagctc gccggcctgt acggcatcgc ggtgggcgcg
     5941 acctcgatgc tgagcatggc cggcatcgtc gtcgcgctgg acgcctacgg gcccatcacc
     6001 gacaacgcgg gcggcatcgc cgagatggcg gaactgcccg aaagcgtgcg cgacgtcacc
     6061 gacccgctgg acgcggtcgg caacacgacc aaggcggtga ccaagggcta cgcgatcggc
     6121 tcggccggcc tggccgcgct ggtgctgttc gccgactaca cgcacggcct cgagggccgc
     6181 ggcatgcacg tcgacttcga gctcgccgac ttccgcgtga tcatcggcct gttcatcggc
     6241 ggcatgatcc cctacctgtt cggcgcgatg gcgatggagg ccgtcggccg cgccgccggc
     6301 tcggtggtcg aggaggtgcg ccgccagttc cgcgacatcg cgggcatcat ggaaggcacc
     6361 ggcaagcccg aatacgggcg cgccgtggac atgctgacct cggccgcgat caaggagatg
     6421 gtcgtgccct cgctgttgcc ggtgatcgcg ccggtgctgg tgggcatgct gctcgggccg
     6481 gcggcgctcg gcggcctgct gatgggcacc atcgtcacgg gcctgttcgt cgcgatctcc
     6541 atgtgcaccg gcggcggcgc gtgggacaac gccaagaagt acatcgagga cggcaacttc
     6601 ggcggcaagg gcagcgaggc ccacaaggcc gcggtgaccg gcgacaccgt cggcgacccg
     6661 tacaaggaca cggcaggccc ggccgtcaac ccgctgatca agatcatcaa catcgtcgcg
     6721 ctgctgatcg tgccgctgct gccggccgcc ggcggcgccg tcgacgcggc caccgaccgc
     6781 cagatcgcgc cgaccccgat cgcggcgccg ctgccggcgc aggaggcctc gccgcc
//
LOCUS       DASVLN010000465         4699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_185318, whole genome shotgun sequence.
ACCESSION   DASVLN010000465 DASVLN010000000
VERSION     DASVLN010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185318"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VF453_11460"
     CDS             <1..613
                     /locus_tag="VF453_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688319.1"
                     /translation="PDRALHAFLAGLRMGSGSVGAPAPDATGAGAGLRLFVRQPFTES
                     GQAQQAIVASVLERIRARDGHPTRFQYLTGCRAESADTFRAAFEQDTGRAFSPEAFRA
                     HRLRQLDQADVFVNIRAGMSESSAFELSYHVFRGACTPILFLVWKQAPIKTTLLKDLG
                     SLCDVTYLEFDDPAELDAPVAEFFRSCERGRRSARPVLARAGA"
     gene            655..2220
                     /locus_tag="VF453_11465"
     CDS             655..2220
                     /locus_tag="VF453_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7688320.1"
                     /translation="MANIKIRTLIIAVLGALMAAVVAIGALGLYGSWRTREAFRDVAL
                     RDRESEIAFTKIRLLMETNRSQVLQALQHNPGFDWAKLHDHPLGVHWALVDQATHDID
                     ALWKRYYGGIASPDEKKLADAWFDKSGGLGIPGVRSAVDAMRGDHWDDAEQVLIKTIN
                     PTYRVGTGASSELSAFLVKRGEANDAAVEAETARLRWILVGSVLASFALAAAAMRALV
                     RGITEPLQQAIAIARRVAAGDLGSRIEAEHRNELGDLLRSLKLMNESLVTIVGSVRDS
                     SDSIAIGAGQIATGNADLSQRTEQQAGSLQQTASSMEELTATVRANADAAQAAEKLAR
                     SASDVAARGGATVATVVSTMDEINASSKKIADIIGVIDGIAFQTNILALNAAVEAARA
                     GEQGRGFAVVAGEVRSLAQRSAQAAREIKALIAASVEKVETGSRQVDEAGRTMEAIVD
                     QVHRVSHLIAEISSATSEQSGGLGQVGDAVSQLDRVTQQNAALVEQSAAAAESLKQQA
                     ARLVGAVAAFKVA"
     gene            complement(2254..4113)
                     /gene="ilvD"
                     /locus_tag="VF453_11470"
     CDS             complement(2254..4113)
                     /gene="ilvD"
                     /locus_tag="VF453_11470"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7688321.1"
                     /translation="MPAYRSKTSTSGRNMAGARSLWRATGMKDDDFSKPIVAVVNSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSREVIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRVNIPVVFVSGGPMEAGKARLAVPGT
                     STVQLKKLDLIDAMVMAADSRMSDADVAEVERSACPTCGSCSGMFTANSMNCLAEALG
                     LALPGNGTVVATHADREQLFRRAGRLVVELTKRWYEQEDARVLPRSVGFEAFENAMTL
                     DIAMGGSTNTILHLLAIAQEAQIPFTMGDIDRLSRTVPQLCKVAPNTNKYHIEDVHRA
                     GGIMAILGELDRAGKLHTGVPTVHAPTMKDALDAWDVVRTQDEAVRTFYRAGPAGIPT
                     QVAFSQATRWPDLDLDRAEGCIRSYEHAYSKEGGLAVLTGNIARDGCVVKTAGVDDSI
                     LVFEGTAHVTESQDEAVENILADRVKAGDVVIVRYEGPKGGPGMQEMLYPTSYIKSKG
                     LGKACALLTDGRFSGGTSGLSIGHCSPEAAAGGAIGLVRDGDRIRIDIPNRTIDVLVS
                     DEELARRRIEQDARGWKPAKPRPRKVSAALKAYAKLVMSADKGAVRDLSQLED"
     gene            complement(4364..>4699)
                     /locus_tag="VF453_11475"
     CDS             complement(4364..>4699)
                     /locus_tag="VF453_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7688322.1"
                     /translation="ADERLAGAIRRLHERPAEPWTVERLAREAALSRSTFFERFNRAV
                     GVAPMAYLLAWRMALAKEQLRRRGSTVTEVAERVGYSSASTFSVAFARHVGVPPARYA
                     REHARLPSG"
BASE COUNT          655 a         1687 c         1703 g          654 t
ORIGIN      
        1 gcccgaccgt gcgctgcacg ccttcctcgc cggcctgcgc atgggcagcg gcagcgtggg
       61 cgcgcccgcg ccggacgcga ccggcgccgg ggcgggcctg cgcctgttcg tgcgccagcc
      121 gttcaccgaa tcggggcagg cgcagcaggc gatcgtcgcg tcggtgctgg agcgcatccg
      181 cgcccgggac ggacatccga cccgcttcca gtacctcacc gggtgccgcg ccgagagcgc
      241 cgacaccttc cgcgccgcgt tcgagcagga caccggccgc gccttctcgc ccgaggcctt
      301 ccgcgcgcac cgcctgcgcc agctcgacca ggccgacgtg ttcgtcaaca tccgcgcggg
      361 catgagcgag agcagcgcct tcgagctctc gtaccacgtg ttccgcggcg cctgcacgcc
      421 gatcctgttc ctggtctgga agcaggcgcc gatcaagacg acgctgctga aggatctcgg
      481 ctcgttgtgc gacgtcacct acctcgagtt cgacgacccg gccgagctcg acgcacccgt
      541 cgccgaattc ttccgcagct gcgagcgcgg acgccgcagc gcgcgacccg tgctggcgcg
      601 cgccggcgcc tgacgcgacc gcatcgaccc gcttgccact tcccccggac catcatggcc
      661 aacatcaaga tccgcaccct catcatcgcc gtcctcggcg cgctcatggc cgccgtcgtc
      721 gccatcggcg cgctcggcct gtacggcagc tggcgcacgc gcgaggcctt ccgcgacgtc
      781 gcgctgcgcg accgcgagtc ggagatcgcc ttcacgaaga tccgcctgct catggagacc
      841 aaccgcagcc aggtgctgca ggcgctgcag cacaaccccg gcttcgactg ggccaagctg
      901 cacgaccatc cgctcggcgt gcactgggcg ctggtcgacc aggcgacgca cgacatcgac
      961 gcgctgtgga agcggtacta cggcggcatc gcgtcgccgg acgagaagaa gctcgccgac
     1021 gcgtggttcg acaagagcgg cggcctgggc atccccggcg tccggtcggc ggtcgacgcc
     1081 atgcgcggcg accactggga cgacgcggag caggtgctga tcaagacgat caacccgacc
     1141 taccgcgtcg gcacgggcgc gtcttccgag ctgtcggcct tcctggtcaa gcgcggcgag
     1201 gccaacgacg cggcggtcga ggccgagacg gcgcgcctgc gctggatcct ggtcggatcg
     1261 gtgctcgcct cgttcgcgct ggcggccgcc gcgatgcgcg cgctggtgcg cggcatcacc
     1321 gagccgctgc agcaggcgat cgcgatcgcg cggcgcgtgg ccgcgggcga cctcggctcg
     1381 cgcatcgagg cggagcaccg caacgagctc ggcgacctgc tgcgctcgct caagctgatg
     1441 aacgagagcc tggtcacgat cgtgggcagc gtccgcgaca gcagcgacag catcgccatc
     1501 ggcgccggcc agatcgccac cggcaacgcc gacctgtcgc agcgcaccga gcagcaggcc
     1561 ggcagcctgc agcagacggc ctcgtcgatg gaggagctga ccgccaccgt gcgcgccaac
     1621 gccgacgcgg cgcaggccgc ggaaaagctc gcgcgctcgg ccagcgacgt ggcggcgcgc
     1681 ggcggcgcga ccgtcgcgac cgtcgtctcg acgatggacg agatcaacgc gtcgtcgaag
     1741 aagatcgccg acatcatcgg cgtcatcgac ggcatcgcgt tccagaccaa catcctggcg
     1801 ctcaacgccg cggtcgaggc cgcgcgcgcg ggcgagcagg gccgcggctt cgccgtcgtg
     1861 gcgggcgagg tgcgcagcct cgcccagcgc agcgcgcagg cggcgcgcga gatcaaggcg
     1921 ctgatcgccg ccagcgtcga gaaggtcgag accggcagcc gccaggtcga cgaggcgggc
     1981 cgcacgatgg aggccatcgt cgaccaggtg catcgcgtca gccacctcat cgccgagatc
     2041 agctccgcga ccagcgagca gtccggcggg ctcggccagg tcggcgacgc ggtctcgcag
     2101 ctcgatcgcg tgacgcagca gaacgcggcg ctcgtcgagc agtccgccgc ggcggccgag
     2161 agcctgaagc agcaggccgc gcgcctggtc ggcgcggtgg cggcgttcaa ggtcgcctga
     2221 gggcggccac gcgcgggcgc gcgcggcctc gggtcagtcc tcgagctgcg acaggtcgcg
     2281 caccgcgccc ttgtcggccg acatcacgag cttcgcgtag gccttgagcg cggccgagac
     2341 cttgcgcggg cgcggcttcg cgggcttcca gccccgggcg tcctgctcga tgcggcggcg
     2401 ggcgagttcc tcgtccgaga ccagcacgtc gatcgtgcga ttcgggatgt cgatgcggat
     2461 gcggtcgccg tcgcgcacca ggccgatcgc gccgccggcg gcggcttccg gcgagcagtg
     2521 gccgatcgac agccccgacg tgccgcccga gaagcggccg tcggtcagca gcgcgcaggc
     2581 cttgcccagg cccttggact tgatgtagct cgtcgggtag agcatctcct gcatgcccgg
     2641 cccgcccttg gggccctcgt agcggacgat cacgacgtcg cccgccttca ccctgtcggc
     2701 caggatgttc tcgaccgcct cgtcctgcga ctcggtgacg tgggccgtgc cctcgaagac
     2761 gaggatgctg tcgtcgacgc cggcggtctt gacgacgcag ccgtcgcggg cgatgttgcc
     2821 ggtcagcacg gccaggccgc cttccttcga gtaggcgtgc tcgtaggagc ggatgcaccc
     2881 ctcggcgcgg tcgaggtcca ggtcgggcca gcgcgtggcc tggctgaagg cgacctgggt
     2941 cgggatgccc gcggggcccg cgcggtagaa ggtgcgcacc gcctcgtcct gcgtgcggac
     3001 gacgtcccag gcgtcgagcg cgtccttcat ggtcggcgcg tgcacggtgg gcacgcccgt
     3061 gtgcagcttg ccggcgcgat cgagctcgcc caggatcgcc atgatgccgc cggcgcggtg
     3121 cacgtcctcg atgtggtact tgttggtgtt gggcgcgacc ttgcacagct gcggcacggt
     3181 gcgcgagagg cggtcgatgt cgcccatcgt gaacgggatc tgcgcctcct gcgcgatcgc
     3241 gagcaggtgc aggatggtgt tggtcgagcc gcccatcgcg atgtccagcg tcatcgcgtt
     3301 ctcgaacgcc tcgaagccca ccgagcgcgg cagcacgcgc gcgtcctcct gctcgtacca
     3361 gcgcttcgtc agctccacca cgaggcggcc ggcgcgcctg aacagctgct cgcggtcggc
     3421 atgcgtcgcg acgacggtgc cgttgcccgg cagcgcgagc cccagcgcct cggccaggca
     3481 gttcatcgag ttggccgtga acatgcccga gcacgagccg caggtcgggc aggccgagcg
     3541 ctccacctcg gcgacgtcgg cgtcgctcat gcggctgtcg gcggccatca ccatcgcgtc
     3601 gatcaggtcg agcttcttca actgcacggt ggacgtgccg ggcactgcca ggcgcgcctt
     3661 gccggcctcc atcgggccgc ccgagacgaa caccacgggg atgttcaccc gcatggcggc
     3721 catcagcatc ccgggcgtga tcttgtcgca gttggagatg cacaccatcg cgtcggcgca
     3781 gtgcgcgttg accatgtact ccaccgagtc cgcgatcacc tcgcggctgg gcagcgagta
     3841 cagcatgccg tcgtggccca tcgcgatgcc gtcgtcgacg gcgatcgtgt tgaactcctt
     3901 ggcgacgccg ccggcggcct cgatctcgcg cgcgacgagc tggccgaggt ccttcaggtg
     3961 cacgtgcccg ggcacgaact gcgtgaacga gttgaccacg gcgacgatcg gcttggagaa
     4021 gtcgtcgtcc ttcatgccgg tggcgcgcca cagggaacgc gcgcccgcca tgttgcggcc
     4081 ggaggtggag gtcttggagc ggtatgcggg catcggttgg cttcgaggag gaggatcgcg
     4141 aaacggaggg acgggccgcg ggatggggcc ctcgcgcgcc cgtgcgaaca acctcttgag
     4201 ccgtgccctg ggcagacagg gggcgattat cgcccgcgcg ggcggcccgc gcaggcctgg
     4261 ggttcacagc gtggatcgca ccggcacgga gaccgccgcg gcgcttcgct cgaacggccg
     4321 cgcgggcgtg gcgccctgcg ggttcgtctc gacgctcccg ggcctatccg gacggcaggc
     4381 gtgcgtgctc gcgcgcgtac cgcgccggcg gcacgccgac gtggcgcgcg aaggcgacgc
     4441 tgaaggtgct cgcggagctg tagccgacgc gctcggcgac ctcggtcacg gtgctgccgc
     4501 ggcgtcgcag ctgctccttg gcgagcgcca tgcgccaggc gagcaggtag gccatcggcg
     4561 cgacgccgac ggcgcggttg aagcgctcga agaaggtcga gcgcgagagc gccgcctcgc
     4621 gcgcgagccg ctccaccgtc cagggctcgg cggggcgctc gtgcaggcgg cggatcgcgc
     4681 cggcgaggcg ctcgtcggc
//
LOCUS       DASVLN010000466         9723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_454684, whole genome shotgun sequence.
ACCESSION   DASVLN010000466 DASVLN010000000
VERSION     DASVLN010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454684"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VF453_11480"
     CDS             <1..225
                     /locus_tag="VF453_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HEX7688323.1"
                     /translation="LGFGLIWHIWWLAVVGFVGMIAAFVVRSFDDDVDYYVPAAEVAR
                     IENRRAAGDDPLASNSLAATESMANRVTPA"
     gene            222..1253
                     /locus_tag="VF453_11485"
     CDS             222..1253
                     /locus_tag="VF453_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7688324.1"
                     /translation="MTDQTANPDESTPRYRSGAVARMIRMPVSTLRVWERRYGVVKPA
                     VSASGHRLYSARDVRRLAQIKQLTETGHAIGSIAALSDEALREVASTHATSLTLHGAA
                     PAPMASLPPWRVVVVGQALARRLQGRALVHGIGRPVHVVAVHDDLAQAAAASPGGPVD
                     ALLLHTPGIDASTLPQAEAAIARLGAQHAAMLYGHGTTTACAEIAAAGVKLLRDAQDD
                     RALAIWLKTVADDFPDERPGAPHAFVAPEFALAADARIPPRRYDDAALADFAGLSSTI
                     ACECPRHVAELLMRLSHFEVYSADCEARSPADAALHAYLGRVAGAARSLLESALERIA
                     IHEGLLRPR"
     gene            1457..1846
                     /locus_tag="VF453_11490"
     CDS             1457..1846
                     /locus_tag="VF453_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HEX7688325.1"
                     /translation="MAALLACFLALDACWLTFVGPHLYRPALDAILAPRVDGLAAVLF
                     YLVYFLGLAVFVVAPELAQRRPDRALARGALFGLVAYGTYDLTNQATLSAWSWSITAA
                     DLSWGAIVSGASSWAAVRTSVGPRGLR"
     gene            1860..3479
                     /locus_tag="VF453_11495"
     CDS             1860..3479
                     /locus_tag="VF453_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HEX7688326.1"
                     /translation="MATTHGSEERPATSTPLRRLVIVLGDQLDIDSSAFDGFDRDRDA
                     VLMAEVQEESTHVWSSRIRIALFLAAMRHFAEVLRSRRRRVEYVALDAPGNSGSLAAE
                     VRRAVLRGRPERLVVTAPGDWRVLKALRDAASDCDVPLEVRDDRHFFGTVREFRSYAR
                     GRKSLRMESFYRQLRERHGILLVDGKPAGGRWNFDTDNRAPFGTGGPPGRPPPVRREP
                     DRITRDVLALVSERFPDHPGSLDDFGWPVTRPQALAALETFVAQRLPDFGRWQDALWP
                     GDPWLWHAQLSAAMNLKLIHPREVVGRVEAAWRDGAVPLPSAEGFIRQVLGWREFVRG
                     VYWTRMPALAAANALDAHEPLPSFYWTGDTEMACLSDALRQTLTHGYAHHIQRLMVTG
                     LFALLLGVEPSQVHAWYLAVYVDAVEWVEMPNTLCMSQYADGGFMTSKPYVASGRYIE
                     RMSSGAYCARCPYRPGLRVGPQACPFTTLYWDFLWRHRQRFSGHPRLGPQVRNAERLD
                     LPEREAIRAHAATIRAATAARAETIHRSHAP"
     gene            3538..4416
                     /locus_tag="VF453_11500"
     CDS             3538..4416
                     /locus_tag="VF453_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX7688327.1"
                     /translation="MVVLGGTGFVGRALCRQLSASGDWRVLVATREATRGDAISMLRN
                     VSVRQVDPLSGSALRQLVASADAVVNLVAILHGSPGQFHAVHVGLPDLLGKTVRTLGG
                     RRVVHVSAIGVDQPRDSLYLQSKSWGERLATEAWPETTVLRPSVIFGKEDRFINTFAR
                     LQRFAPFVPLACSDARFQPVWVEDVARAIVAGLHRPDTRGRTYECAGPDVLTLHELVR
                     RAGQWAGVARPILALPLPIARAQAWLMERLPGEPLMSRDNLRSMQTPNVAAPGAPSLR
                     DLGIAARSLDDYLSRG"
     gene            complement(4451..5404)
                     /locus_tag="VF453_11505"
     CDS             complement(4451..5404)
                     /locus_tag="VF453_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7688328.1"
                     /translation="MDPLSSVIGLLRPLGTTVGATDVGGDIAIGFAAHEGAFFYSVAA
                     GRCWLAVDGVAEPLLLRAGDCIVLPSGRPFTLASDLRLAPIPAERVFEGRPNGSIAVY
                     NGGGRCTMFAAHFEFQADASRFLLGALPPVVAVRDAQARAALRATLDQMIDELRCAAP
                     GCDAIVEHLAHIALVKVLRFHLSEAGGERPGWLFALADPRMGAVMAAMHADLARAWTV
                     GELAEVGAMSRTTFATRFKSIVGVAPMAYLASLRMLTAVKRLGEPGARTSDVAIATGY
                     QSEAAFNVAFKRVLGLPPRRYMSLHSEASAVTRGPFHQDPG"
     gene            5622..6677
                     /locus_tag="VF453_11510"
     CDS             5622..6677
                     /locus_tag="VF453_11510"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX7688329.1"
                     /translation="MSAPGPNEVLIRNRAVALNPFDRIVQTLGGLVTPWLDYPCILGS
                     DVAGEVVAVGTGVSRFKPGDRVAGLALGVDKVSNRPAEGAFQHYVLLREDATTAIPEA
                     MSFERAAVLPLAIATAASGLFLADQLGLERPRHPASGRRDGTVIVWGGSTSVGSCAIQ
                     LARAAGYDVATTASPRNFEHVRRLGASLAFDYRDPAVMAHMTDALRGRQVVGALAIGA
                     GSGKACIELVRRAGGRRIVSMASAPLPLDGAPLGSQRAWKLATLPRLALGFAGLALRA
                     RLAGVTVRSLWGTALVKDELGRHIFRDFLGPALAQGVVVPAPEPLVSGHDLRDIPAAM
                     ELLRRGVSARKIVVALG"
     gene            6810..7349
                     /locus_tag="VF453_11515"
     CDS             6810..7349
                     /locus_tag="VF453_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX7688330.1"
                     /translation="MRIRTILSSAIIAMLAACATVPDTAPPLTLATHVDLPRFMGDWY
                     VIASIPTIFEDGAHNAKDTYALRPDGTIATTFTFNAGAPDGPVKHYESIGHVLDRSSN
                     AVWGQRYIWPFQADYRISYVSPEYDVTVVTRAKRDHVWIMARAPSLPDAQLARLTAFV
                     ASQGYDVAKLRRVPQIGAQ"
     gene            complement(7414..8370)
                     /locus_tag="VF453_11520"
     CDS             complement(7414..8370)
                     /locus_tag="VF453_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688331.1"
                     /translation="MDQLLALRVFARIAETGAFARAADTLAIPRPTVTKLVQDLESHL
                     GTKLLQRTTRRVSLTQEGAAYYERAARLLVEVDEMDAQAASARVRLRGRLRVDVGSSI
                     ANVILIPALPGFRARYPDIALELGVNDRPVDLIGDNVDCVIRSGTLADTSLVARRLAD
                     LAWVTCASPVYLGARGIPAHPAELAAGRRGGDAHRARGHVVASYFSSLTGRPFPLHFQ
                     KGGDKLVIEPQADVGVNESTAHVTALLAGLGVGQTFRFMVDPLIRSGALKSVLEDWSR
                     PHQPLHLVHPSARHLSAKLRAFSDWLVEIFAPFDAGRAGPRG"
     gene            8478..9233
                     /locus_tag="VF453_11525"
     CDS             8478..9233
                     /locus_tag="VF453_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7688332.1"
                     /translation="MSQQLTDKIAVITGATTGIGLATAKAFAREGATVFITGRRQAEL
                     DAAVAAIGPSATGVRADSSRMEDLDALFDLVRRRHGRIDVLFANAGGGSMLPLGQITE
                     EHFDDIFGRNVKGVLFTVQKALPLLATGASVILTGSTTSIEGTANFSVYSASKAAVRN
                     FARSWILDLKDRGIRVNTIAPGATETPGLVELAGSDRARQQGLLDHLASRVPLGRVGR
                     PEEIANAAVFLASDASSFVNGIELFVDGGQAQV"
     gene            9267..>9723
                     /locus_tag="VF453_11530"
     CDS             9267..>9723
                     /locus_tag="VF453_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX7688333.1"
                     /translation="MPTNVVFGAFDRHNLGDLLLAHVVAALLPRGEPLVFAGLAARDL
                     RPFGGHLVEPLHALRDDPRLRGARLVHAGGEILACTARQAATMLLPPSEVDATVASLD
                     AHPEQACAWRRAMLGTDDEMPYVAARAGLPALGRVVFAGVGGVTLDALPA"
BASE COUNT         1369 a         3536 c         3387 g         1431 t
ORIGIN      
        1 ctgggcttcg gcctgatctg gcacatctgg tggctggccg tcgtcggctt cgtcggcatg
       61 atcgccgcct tcgtcgtgcg ctcgttcgac gacgacgtgg actactacgt gccggccgcc
      121 gaggtcgcgc gcatcgagaa ccgacgggcg gcaggcgatg atcccttggc gtcgaattcg
      181 cttgccgcca ccgagtccat ggccaaccga gtcacgccag catgaccgac cagacagcga
      241 atcccgacga gtcgacccct cgctaccgca gcggcgcggt cgcgcggatg atccggatgc
      301 cggtttccac cttgagggtc tgggagcgcc gctacggcgt cgtgaagccc gcggtctcgg
      361 cgtccggcca ccggctgtac tcggctcggg acgtgcgccg gctggcgcag atcaagcagc
      421 tgaccgagac cggacatgcc atcggctcga tcgcggcgtt gagcgacgag gcgttgcgcg
      481 aggtggcatc cacgcacgcg acctcgttga ccctgcacgg cgcggctccg gcgcccatgg
      541 ccagcctgcc gccatggcgg gtcgtcgtcg tcggccaggc cctggcacgc cggctgcagg
      601 gacgcgcgct ggtgcacggg atcgggcgcc ccgtgcacgt cgtcgccgtt catgacgatc
      661 tcgcgcaggc cgccgcggca tcgccgggcg gacccgtcga cgcgctgctg ctgcacacgc
      721 cggggataga cgcttcgacc ctgccgcagg ccgaagccgc gatcgcgcgc ctcggcgcgc
      781 agcatgcggc gatgctgtac ggtcatggca ccacgaccgc ctgcgcggag atcgccgcgg
      841 ccggggtcaa gctgcttcgc gatgcccagg acgaccgtgc gctggccatc tggctcaaga
      901 ccgtggccga cgatttcccc gacgagcggc ccggcgcacc gcacgccttc gtggcgcccg
      961 aattcgcgct cgcggcggac gcgcgcatcc cgccgcgacg ctacgacgat gcggcgctgg
     1021 cggacttcgc cggcttgtcg tcgacgatcg cctgcgagtg ccctcgccac gtcgcggagc
     1081 tgctgatgcg gctgtcgcac ttcgaggtct acagcgccga ttgcgaggca cgaagcccgg
     1141 cggacgccgc gctccacgcc tacctcggcc gcgtcgccgg cgcggcgcgc agcctgctcg
     1201 aatcggcgct ggagcgcatc gcgatccacg aggggctgct gcgaccccgc tgaggcggag
     1261 cggcgacgtc ttcgcggcgt gtacgccggc tgacggggct ccacgcgaca ggagcacccg
     1321 cgcaaatcca agtcgggcgc aagtgcgtaa tcccagggaa tctccccttc cgatctccca
     1381 tcgcccgccg cccgcggccg ccgttcctcg acaggagccc gatatgccga ttccgccgcg
     1441 tcacctggcg cctgccatgg ccgcactgct cgcatgcttc ctggcgctgg acgcctgctg
     1501 gctcaccttc gtcggccctc atctgtaccg cccggcgctc gacgcgatcc tcgcgccgag
     1561 ggtcgacggg ctcgcggccg tgctgttcta cctcgtgtat ttcctgggtc tcgccgtgtt
     1621 cgtggtggcg ccggagctgg cccagcgtcg tcccgatcga gcactggcga ggggcgcgct
     1681 cttcggcctc gtggcgtatg gcacctatga cctgaccaac caggcgacgc tgtccgcatg
     1741 gtcgtggagc atcacggcgg ccgatctctc gtggggggcg atcgtcagcg gtgcatcgtc
     1801 gtgggcggcg gtccggactt ccgtcgggcc gcgagggctt cgctaggctc gcccacgcca
     1861 tggcgacgac gcacggctcc gaggagcgcc cggcgacgag cacgccgctg cgccggctcg
     1921 tcatcgtgct gggcgatcag ctcgacatcg actcgagcgc cttcgacggc ttcgatcggg
     1981 atcgcgacgc ggtgctcatg gccgaagtgc aggaggaatc cacgcacgtc tggtcgagcc
     2041 gcatccggat cgccctgttc ctcgcggcca tgcggcactt cgcggaggtt ctacggtcgc
     2101 gccggcggcg cgtcgaatac gtcgcgctgg acgcgcccgg caactcgggc agcctggccg
     2161 ccgaggtgcg gcgtgccgtc ctccgagggc gacccgagcg gctcgtggtg accgcgcccg
     2221 gcgactggcg cgtcctcaag gccctgcggg acgccgccag cgattgcgac gtgccgctgg
     2281 aggtgcgcga cgaccgtcac ttcttcggca cggtacgcga gttccggagc tacgcgcggg
     2341 gccgcaagtc cctgcggatg gagagcttct accgccagct gcgcgagcgg cacggcatcc
     2401 tgttggtcga cggcaagccg gcgggcggtc gctggaattt cgacacggac aaccgggcgc
     2461 cgttcggcac cggcggcccg ccagggcggc cgccgccggt gcgccgggag ccggaccgca
     2521 tcactcggga cgtcctggcg ctcgtctccg agcgcttccc cgatcatccc gggtcgctgg
     2581 acgacttcgg atggcccgtg acgaggccgc aggccctcgc ggccctggag accttcgtcg
     2641 cgcagcgctt gcccgacttc ggccgatggc aggatgccct ctggcctggc gatccctggc
     2701 tctggcatgc gcagctctcc gccgcgatga acctgaagct gatccatccc agggaggtgg
     2761 tcggccgcgt ggaggccgcg tggcgcgacg gcgcggtgcc gttgccgagc gccgaagggt
     2821 tcatccgcca ggtcctcggc tggcgggagt tcgtgcgcgg cgtctactgg acgcgcatgc
     2881 ccgccctggc cgccgccaac gcactggacg cccacgagcc cctgccctcg ttctactgga
     2941 cgggcgacac cgagatggcg tgcctgtccg acgccttgcg ccagacgctg acccacggct
     3001 acgcccacca catccagcga ctcatggtga cggggctgtt cgcgctgctg ctgggggtcg
     3061 agccgtcgca ggtccatgcg tggtacctgg ccgtctacgt cgatgcggtc gaatgggtcg
     3121 agatgcccaa cacgctgtgc atgagccagt acgccgacgg aggcttcatg accagcaagc
     3181 cctatgtcgc gagcggccgc tacatcgagc gcatgagcag cggcgcgtac tgcgcgcgct
     3241 gcccgtaccg gccgggcctg cgggtcgggc cgcaagcgtg tcccttcacc accttgtact
     3301 gggacttcct ctggcgccac aggcaacgct tctccggtca tccgcgcctg ggcccacagg
     3361 tcaggaacgc ggagcgtctc gacctgcccg aacgcgaggc gatccgtgcg catgcggcaa
     3421 cgatacgcgc cgccacggcg gctcgcgctg aaaccatcca tcgatcccat gccccctgag
     3481 ccgatgaacg cacgcgggcg acccgccgcc cggcccgatc gagacggagt gccgaccgtg
     3541 gtcgtcctcg gcggcaccgg cttcgtgggt cgagccctgt gccgccagct gtcggcgagc
     3601 ggcgattggc gcgtcctcgt ggcgacgcgc gaggcgacgc gtggcgacgc gatctccatg
     3661 ctgcgcaacg tgtccgtccg gcaggtcgat ccgctgagcg gctccgctct gcggcagctc
     3721 gtcgcgtccg ccgacgccgt ggtcaacctc gtcgccatcc tccacggctc ccccgggcag
     3781 ttccacgccg tgcacgtcgg gctgcccgac ctgctcggaa agacggtgcg cacgctgggt
     3841 ggaaggcgcg tcgtccatgt cagcgcgatc ggcgtcgacc agccgcggga cagcctgtac
     3901 ctgcagtcca agtcctgggg cgagcggctc gcgacggagg catggccgga gacgaccgtc
     3961 ctgcgcccgt ccgtgatctt cggcaaggag gaccggttca tcaacacctt cgcgcgcctg
     4021 cagcgcttcg cgccgttcgt tccgctcgcc tgcagcgacg cgcgcttcca gcccgtgtgg
     4081 gtcgaggacg tggcccgcgc gatcgtcgcc ggcctgcacc ggcccgacac ccgggggcgc
     4141 acctacgagt gcgcgggccc ggacgtgttg acgctgcatg agctcgtgcg gcgggcgggg
     4201 cagtgggcag gcgtcgcccg gcctatcctt gcgctgccgc tgccaatcgc acgcgcgcag
     4261 gcctggctca tggagcgact gcccggcgag ccgctgatgt cgcgcgacaa cctgcgatcg
     4321 atgcagacgc ccaacgtggc cgcgccgggc gcgccgtccc tgcgcgatct cggcatcgcc
     4381 gcccgctcgc tcgacgacta tctctctcgc gggtgacatc ccgaaccgcg acccgtgcag
     4441 gcgggccgag ctaaccgggg tcttggtgga acggcccgcg cgtcacggcg gacgcctcgg
     4501 aatgcaggct catgtaccgc cggggcggca gcccgagcac ccgcttgaac gccacgttga
     4561 acgcggcctc cgactggtag ccggtcgcga tcgccacatc cgatgtccgc gcaccgggct
     4621 ccccgaggcg cttgaccgcc gtcagcatcc tgagcgatgc caggtaggcc atgggcgcga
     4681 cgccgacgat cgacttgaac cgggtggcga acgtggtccg ggacatggcc ccgacctcgg
     4741 ccagctcccc gacggtccac gcccgggcca ggtcggcgtg catcgcggcc atcacggcgc
     4801 ccatccgggg atcggcgagc gcgaagagcc agcccggacg ctcgccaccc gcttcggaca
     4861 ggtggaagcg aagcaccttc accaacgcga tgtgcgcgag atgctccacg atcgcatcgc
     4921 agccgggtgc ggcgcatcgc aactcgtcga tcatctggtc gagcgtcgcg cgcagcgcgg
     4981 cccgcgcctg ggcatcccgg accgccacca ccggaggcag cgcgccgagc aggaagcgcg
     5041 acgcatcggc ctggaattcg aagtgcgccg cgaacatcgt gcaacggccg cctccgttgt
     5101 agacggcgat ggagccgttc ggccgaccct cgaagacgcg ctccgccggg atcggcgcaa
     5161 gccgcaggtc gctcgcgagc gtgaacggcc gcccgctcgg caggacgatg cagtccccgg
     5221 ctcgcaggag aaggggttcg gccacgccgt ccacggccaa ccagcatcgc ccggcagcga
     5281 cggaatagaa gaacgcacct tcatgcgctg cgaatccgat cgcgatgtcg cccccgacgt
     5341 cggtggcgcc cacggtggtg ccgagcgggc gcaggagccc tatcacgctc gagagcgggt
     5401 ccatggtatt cgaacgatcg cgaaaaagat gcggagccct gaatatagat cgttccagcg
     5461 cgtcgcccta gagtcctcga cgcgcccggg ccttggacaa gcgaggccgg gcgcgtcgca
     5521 ctccctgcat cccatccccg aagaaagccg ccatcaccat gatcaaccat gccgcctggt
     5581 tgcgctcgcg gacacaggcc ctcgcctttt ccgaggcgcc catgagcgcg ccgggtccga
     5641 acgaagtcct gatccgcaat cgcgccgtcg ccttgaaccc gttcgaccgg atcgtgcaga
     5701 cgctcggagg cctcgtgacg ccgtggctcg actatccgtg catcctcggc tccgatgtcg
     5761 ccggcgaagt cgtcgcggtc ggcaccggcg tctcccgctt caagccgggg gatcgtgtcg
     5821 ccggtctcgc cctgggcgtc gacaaggtgt ccaaccgacc cgcggaaggg gcgttccagc
     5881 actacgtcct cctgcgcgag gacgccacga cggccattcc cgaggcgatg tcgttcgagc
     5941 gcgccgccgt gctgccgctc gcgatcgcca ccgccgccag cggcctgttc ctggcggacc
     6001 agctgggcct cgagcggccc cgccatccgg cgtccggccg gcgggacggc accgtgatcg
     6061 tgtggggcgg ctcgaccagc gtgggcagct gcgccatcca gctcgccagg gccgccggct
     6121 acgacgtggc gaccacggcc tcgccgcgca acttcgagca tgtgcgtcgc ctcggcgcct
     6181 cgctggcctt cgactatcgc gatcccgcgg tcatggcgca catgaccgac gcgctgcgtg
     6241 gacggcaggt cgtgggcgcc ctcgccatcg gtgcgggatc cggcaaggcg tgcatcgagc
     6301 tcgtgcgtcg cgcgggcggc cgcaggatcg tgtcgatggc cagtgccccg ctgccgctcg
     6361 acggcgcgcc gctcggcagc cagcgggcct ggaagctcgc gacactgcct cgcctggcgc
     6421 tgggattcgc ggggctggcc cttcgcgcac gcctcgccgg cgtgacggtg cgttccctct
     6481 ggggcacggc cctcgtcaag gatgagctgg gacgccacat cttccgcgac ttcctcgggc
     6541 ccgcgctggc gcagggcgtc gtggtgcccg cgccggagcc gctcgtcagc ggccatgacc
     6601 ttcgcgacat ccccgccgcc atggagctcc tgcgccgggg tgtctcggcc aggaagatcg
     6661 tggtcgcgct cgggtgacgg cgctcgccgc gagaccgcca cggtcgcgcg cgggcatggg
     6721 tcgcgcagag atccaagcgg gccgcccgat cgtaagcccg ggatcgaccg cgcgtcgcag
     6781 gtcgaaccca ccgcaccggg gagcattgca tgcgaatccg aaccatcctg tcgagcgcga
     6841 tcatcgccat gctggcggcg tgcgcgaccg tgcccgacac cgcgccgccg ctgacgctgg
     6901 caacgcacgt cgacctgccc cggttcatgg gcgactggta cgtgatcgcc agcatcccga
     6961 cgatcttcga ggacggcgcg cacaacgcga aggacacgta cgcgctgcgg ccggacggga
     7021 cgatcgcgac gacgttcacg ttcaacgcgg gtgcgcccga cgggcccgtc aagcactacg
     7081 aatcgatcgg gcacgtcctc gaccggtcgt cgaacgccgt ctggggacag cgctacatct
     7141 ggccgttcca ggccgactac cggatctcct atgtctcgcc ggagtacgac gtgaccgtcg
     7201 tcacgcgcgc caagcgcgac catgtctgga tcatggcgcg cgcaccgtcc ctgcccgacg
     7261 cgcaattggc tcgcctcacc gcgttcgtgg catcccaagg gtacgatgtc gccaagttga
     7321 ggcgcgtccc gcaaatcggc gcgcagtagt cggcacccag cagtcgacgg accgccggct
     7381 gccgccggtg ttcgccagcg agcgaccgcg ggatcagccc cgcgggccgg cgcgcccggc
     7441 atcgaacggc gcgaagatct ccaccagcca gtccgagaac gcgcgcagct tggcgctcaa
     7501 gtgccgtgcg ctgggatgga ccaggtgcag gggctggtgc ggacgcgacc agtcctcgag
     7561 cacggacttc agggcgccgc ttcggatcag cgggtcgacc atgaatcgga acgtctggcc
     7621 gacgccgagg ccggcgagca gcgccgtgac gtgcgccgtg ctctcgttca cgccgacgtc
     7681 ggcctggggc tcgatgacga gcttgtcgcc gcccttctgg aagtgcagcg gaaacggacg
     7741 gcctgtcagc gacgagaagt agctggcgac gacatgtccg cgggcccggt gcgcatcgcc
     7801 gccgcgcctg ccggccgcca gctccgcggg atgagcggga attccccgcg cgccgaggta
     7861 gaccgggctc gcgcaggtca cccacgccag atcggcgagg cggcgggcca cgagcgaggt
     7921 gtcggccagc gtgcccgatc ggatgacgca gtcgacgttg tcgccgatca ggtccacggg
     7981 ccggtcgttg acgcccagct ccagcgcgat gtcgggatag cgggcgcgaa agccgggcag
     8041 cgcggggatc aggatcacgt tggcgatcga ggagccgacg tccacccgca gccgtccgcg
     8101 caggcgcacg cgggcgctgg cggcctgggc gtccatctcg tcgacctcga ccagcagccg
     8161 cgccgcacgc tcgtagtagg ccgccccttc ctgcgtgagg ctgacgcggc gggtcgtgcg
     8221 ctgaaggagc ttggtgccca gatggctctc caggtcctgc accagcttcg tgacggtggg
     8281 ccgcggaatc gccagcgtgt cggcggctcg ggcgaaggcg ccggtctcgg cgatgcgcgc
     8341 gaacacgcgg agggcaagaa gctggtccat ccccgattat tcatcgacat gaattaagtt
     8401 gtccgattcg gcggctttat tcgtatcacg gaacaaccta tcgtgcagtc accccatcaa
     8461 cttcctggag caacgacatg agccagcaac tcacggacaa gatcgccgtc atcaccggag
     8521 ccaccacggg catcggcctg gcgacggcca aggcattcgc ccgcgaaggc gcgaccgtct
     8581 tcatcacggg ccgccgccag gccgagctcg acgcggcggt cgcagcgatc ggcccgagcg
     8641 cgaccggcgt gcgcgccgac tcgtcccgca tggaggacct ggatgcgctc ttcgacctcg
     8701 tgcgccgccg ccacggccgc atcgacgtcc tgttcgcgaa cgccggcggc ggctcgatgc
     8761 tgccgctggg ccagatcacg gaggaacact tcgacgacat cttcggtcgc aacgtcaagg
     8821 gcgtcctgtt caccgtgcag aaggcgctgc cgctgctggc cacgggggcc tcggtgatcc
     8881 tgaccggctc gacgaccagc atcgagggaa cggcgaactt cagcgtctac agcgcgagca
     8941 aggccgccgt gcgcaatttc gcccgcagct ggatcctcga cctgaaggat cgcgggatcc
     9001 gggtgaacac gatcgccccc ggtgcgaccg agacgccggg tctggtcgag ctcgccggat
     9061 ccgaccgcgc acggcagcag ggcctgctgg accacctcgc ctcccgcgtg ccgctgggac
     9121 gagtgggccg cccggaggag atcgccaacg cggcggtctt cctggcatcg gatgcctcga
     9181 gcttcgtcaa tggcatcgag ctgttcgtcg acgggggcca ggcgcaggtc tgagggcgcc
     9241 ggcgagcgtg cgatgctgtg gccgccatgc ccacgaacgt cgtcttcggc gccttcgacc
     9301 gccacaacct gggcgacctg ctgctcgcgc acgtcgtcgc ggcgctgctg ccgcgcggcg
     9361 agccgctcgt gttcgccggc cttgcggcgc gtgacctgcg gcccttcggc ggccacctgg
     9421 tcgagccgct gcacgcgctg cgcgacgatc cgcgcctgcg cggcgcgcgg ctggtgcatg
     9481 cgggcggcga gatcctcgcc tgcaccgcgc gccaggcggc gacgatgctg ctgccgccgt
     9541 ccgaggtcga cgccaccgtg gcctccctcg atgcgcatcc cgagcaggcg tgtgcctggc
     9601 gtcgcgcgat gctcggcacg gacgacgaga tgccctacgt cgccgcgcga gccggcctgc
     9661 cggcgctcgg gcgcgtcgtg ttcgcgggcg tcggcggcgt gacgctggac gccctgccgg
     9721 ccg
//
LOCUS       DASVLN010000467         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_78279, whole genome shotgun sequence.
ACCESSION   DASVLN010000467 DASVLN010000000
VERSION     DASVLN010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78279"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VF453_11535"
     CDS             <1..673
                     /locus_tag="VF453_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7688334.1"
                     /translation="VLQARRARAAALAAAQAESALARAELAVISGKLNPHFLFNTLNS
                     LIALTRKDARAAEDGLLRFAGLLRYVLDTKRSAADRVALADEIEFVRDYLALESLRLG
                     PRLRVEWDVDPATLDDEIPPLSLQPLVENSIVHGVAPRVRGGVVRIRSGRRGTDELAL
                     SVEDDGPGCDPAALDEDAPPAARRGIGLASLRRRFALDYDGRARLDVRTAPGAGFRVD
                     LRIPL"
     gene            670..1446
                     /locus_tag="VF453_11540"
     CDS             670..1446
                     /locus_tag="VF453_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7688335.1"
                     /translation="MTERTRVLIAEDEPLAAESLADWVAALPRLELLETCADGETALE
                     RIRALRPGLVLMDIQMPGLTGLQVLRALAQDEGPRPAVIFTTAYDEHALAAFELHAVD
                     YLLKPFSYERFVEAVEHALQARAAAPGGVGLDEALGAASAALAPAAAQPLARILVRDQ
                     GRIFPIQVDAIEYLRSDTKYTALVSRGRSFLVRLPINAFEQRLDPARFLKLHRSCIVN
                     LDFVDAMTPDESSQLVVQMRDGTRITANRDVSRKLREQSI"
BASE COUNT          199 a          599 c          531 g          200 t
ORIGIN      
        1 ggtgctgcag gcgcgccgcg cccgcgccgc cgcgctcgcc gcggcgcagg ccgaatcggc
       61 gctggcgcgc gccgagctcg ccgtgatcag cggcaagctc aacccgcact tcctcttcaa
      121 caccctgaac tccctcatcg cgctgacgcg caaggatgcc cgcgccgccg aggacgggct
      181 gctgcgcttc gccggcctgc tgcgctacgt gctggacacc aagcgcagcg ccgccgaccg
      241 cgtcgccctc gccgacgaga tcgagttcgt gcgcgactac ctcgccctgg aatcgctgcg
      301 cctgggcccg cggctgcgcg tggaatggga cgtcgacccg gccacgctgg acgacgagat
      361 cccgccgctc agcctgcagc cgctggtgga gaacagcatc gtgcacggcg tcgcgccccg
      421 ggtgcgcggc ggtgtggtgc gcatccgcag cggccgccgg gggacggacg agctcgccct
      481 cagcgtggag gacgacggcc ccggctgcga ccccgccgcg ctcgacgagg acgcgccgcc
      541 cgcggcgcgc cgcggcatcg gcctggcgtc gctgcggcgt cgcttcgccc tcgactacga
      601 cggccgcgcc cgcctcgacg tgcgcaccgc ccccggcgcg ggcttccgcg tcgacctccg
      661 gatcccgcta tgaccgaacg aaccagggta ttgatcgccg aggacgaacc gctcgcggcc
      721 gagtcgctcg ccgactgggt ggccgcgctg ccgcggctcg agctgctgga gacctgcgcc
      781 gacggcgaga ccgcgctcga acgcatccgc gcgctgcggc cgggcctggt gctgatggac
      841 atccagatgc ccggcctcac cggcctgcag gtgctgcgcg ccctcgcgca ggacgagggc
      901 ccgcgcccgg cggtgatctt caccacggcc tacgacgagc acgccctcgc cgccttcgag
      961 ctgcatgcgg tcgactacct gctcaagccg ttctcgtacg agcgcttcgt cgaggcggtg
     1021 gagcatgcgc tgcaggcgcg cgccgcggcc cccggcggcg tcgggctgga cgaggcgctg
     1081 ggagcggcct cggcggcgct cgcgccggcc gccgcgcagc cgctggcgcg catcctggtg
     1141 cgcgaccagg gccggatctt ccccatccag gtcgacgcca tcgagtacct gcgctcggac
     1201 accaagtaca ccgcgctcgt gagccgcggc cgcagcttcc tcgtgcggct gccgatcaac
     1261 gcgttcgagc agcggctcga cccggcgcgt ttcctgaagc tgcaccgcag ctgcatcgtc
     1321 aacctcgact tcgtcgacgc gatgacgccg gacgagagct cgcaactcgt cgtgcagatg
     1381 cgcgacggca cgcgcatcac cgccaaccgg gacgtctccc gcaagctcag ggagcaatcg
     1441 atatgacgat ccgcaccgaa tcgcccgccc gcctggcgct ggccgtcgcg gccgtgttcc
     1501 tcgccggcgg cgcgtgcgcg cagagatcg
//
LOCUS       DASVLN010000468         4705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_345720, whole genome shotgun sequence.
ACCESSION   DASVLN010000468 DASVLN010000000
VERSION     DASVLN010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345720"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VF453_11545"
     CDS             <1..433
                     /locus_tag="VF453_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEX7688336.1"
                     /translation="ASPLDYPVASTAAGADADLGLCRSGRAGLPVDPADCGRFRAPTL
                     RNVAIRASFFHNGSIASLRDAVAFYATRDTDPGRWYSRNADGSVHVYDDIPAALRASV
                     DHGIPFEPQPGGRPRLSEQDVDDLVAFLRTLTDADVQVLAK"
     gene            complement(441..1379)
                     /locus_tag="VF453_11550"
     CDS             complement(441..1379)
                     /locus_tag="VF453_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7688337.1"
                     /translation="MQGPSPSSRRAGTATAYAMLCVVMALWAGNSIVGRAVRDTIPPF
                     TLAFARWAGALCLVGPFAARRVAADGAMLARHWRIVLLLGLVGVAAFNGFLYSGLRLT
                     TATNGLLLQATVPALVLLFGVLLRQSRAPRAQVAGVSLSTFGVALVVFRADPSAVATL
                     RFNTGDALVLCGCVAWALYTALLPRRPAVHPLAFLAVTFAIGALAMLPLAAAEWQRVH
                     IAWTPATLGAFAYVAVFPSCVAYLLYNEAVSAIGAGPAGQAMSLMPLFGAGLASLVLG
                     EPLHAWHLAGMALICAGIGATVLGLRRAARRQLAGA"
     gene            1552..1896
                     /locus_tag="VF453_11555"
     CDS             1552..1896
                     /locus_tag="VF453_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688338.1"
                     /translation="MFIMFGWRKETRELPGDLRCHCYRCQRPRGWEHWKETEWVSFFM
                     IKTIPFLSRSHVVCAACRESFAIDGRRARDLAAGRGLPALAAHVEGLQLAGKTEVQRR
                     FLLAQREHHPPQ"
     gene            1949..2509
                     /locus_tag="VF453_11560"
     CDS             1949..2509
                     /locus_tag="VF453_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEX7688339.1"
                     /translation="MDETYVSTDVETDGPIPGPHSMLSLGSAAYTADKRLLGTFSANL
                     ETLPGASPHPQTAAWWATQPEAWAACRRDLEAPEAALRRYAAWIATLPGRPVFVAYPA
                     GFDFLFVYWYLIRFTGASPFSHSALDMKTFAMAVLGTGYRESTKNRMPRDWFDPLPHT
                     HVALDDAIEQGALFCNMLAASRKPAA"
     gene            complement(2525..3457)
                     /gene="argF"
                     /locus_tag="VF453_11565"
     CDS             complement(2525..3457)
                     /gene="argF"
                     /locus_tag="VF453_11565"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX7688340.1"
                     /translation="MKPGNSLMKHYLQFKDLRAEEYAYLFERTREIKKRFKNYEKYQP
                     LVDRTLAMIFEKASTRTRVSFEAGMYQMGGSVVNLTTGDSQLGRAEPIEDSARVISRM
                     VDLVMIRTFEQTKIERFAAHSRVPVINGLTNEFHPCQILADLFTFIEHRGAIAGKTVA
                     WVGDGNNMANTWLQAADILGFTLHVSTPSGYEIDAEVAGVRRADCVKVFDHPLDACRD
                     ADLVTTDVWTSMGYEAENAKRMEAFAQWRVDAGMMAVARPDALFMHCLPAHRGEEVTA
                     DVIDGPQSVVWDEAENRMHVQKALMEYLLLGRIG"
     gene            complement(3454..4650)
                     /locus_tag="VF453_11570"
     CDS             complement(3454..4650)
                     /locus_tag="VF453_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7688341.1"
                     /translation="MSTPTAMPHVMNTYGRLPFALSHGRGCRVWDTEGREYLDGLAGI
                     AVNTLGHAHPQLVAAIADQAGKIIHSSNYYQVPLQETLAAMLCERSGLAAAFFCSTGL
                     EANEGLIKLARKYGHGKGVARPEIVVCERAFHGRSLATLSATGNPKIQEGFGPLVEGF
                     VRVPFNDAAAVRAVAESNPDVVAVMLEPVQGEGGINPARHEYLRELRAICDERGWLLM
                     LDEIQCGIARTGKWFGFQWSGIVPDAMSLAKGLGSGVPVGAFLAGERAKDVLQPGNHG
                     TTFGGNPLAMRAAVETLRLIESEGLIDNAARVGAHLKAALQRELGGLPGVKEVRGEGL
                     MIGVELARPCGAVLTRAAEAGLLISVTADSVIRIVPALILTTAEADEIVARLAPVVKA
                     FLAEQA"
BASE COUNT          673 a         1702 c         1685 g          645 t
ORIGIN      
        1 ggcgtcgccg ctcgactatc ccgtcgcgtc cacggccgcc ggcgccgacg ccgacctcgg
       61 cctgtgccgc tcgggccgcg ccggcctgcc ggtcgacccc gccgactgcg gccgcttccg
      121 ggcgcccacg ctgcgcaacg tcgcgatccg cgcgagcttc ttccacaacg gctcgatcgc
      181 gtccctgcgc gacgccgtcg ccttctacgc cacgcgcgac accgacccgg gccgctggta
      241 ttcgcgcaac gccgacggca gcgtccacgt gtacgacgac atccccgccg cgctgcgggc
      301 gtcggtcgac cacgggatcc cgttcgagcc gcagcccggc ggccggccgc gcctgagcga
      361 gcaggacgtc gacgacctcg tggcgttcct gcggacgctg accgacgcgg acgtgcaggt
      421 cctcgcgaaa tgagcggccc tcaggcgccc gccagctggc gccgcgcggc cctgcgcagc
      481 ccgagcaccg tcgcgccgat ccccgcgcag atcagcgcca tgcccgccag gtgccaggcg
      541 tgcagcggct cgccgagcac cagcgacgcg agccccgcgc cgaacagcgg catcaggctc
      601 atcgcctggc ccgcggggcc ggcgcctatc gccgagacgg cctcgttgta gagcaggtac
      661 gcgacgcacg acgggaagac cgcgacatag gcgaacgcgc cgagagtcgc cggcgtccag
      721 gcgatgtgga cgcgttgcca ctccgcggcc gccagcggca gcatcgcgag cgcgccgatc
      781 gcgaaggtga ccgcgaggaa ggcgagggga tgcacggcgg ggcggcgcgg cagcagcgcg
      841 gtgtagagcg cccaggcgac gcagccgcac agcacgaggg cgtcgccggt gttgaagcgc
      901 agcgtggcga cggccgaagg atcggcgcgg aagacgacca gcgcgacgcc gaaggtcgac
      961 agcgacacgc ccgcgacctg cgcccgcggc gccctcgact ggcgcagcag gacgccgaac
     1021 agcagcacga gtgccggcac cgtcgcctgc agcagcagtc cgttggtcgc ggtcgtcagc
     1081 cgcagccccg aatacaggaa gccgttgaac gcggcgacgc ccaccagccc gagcagcagc
     1141 acgatgcgcc agtgccgtgc cagcatcgcc ccgtcggcgg ccacgcgcct tgccgcgaag
     1201 ggccccacca ggcacagcgc cccggcccag cgcgcgaacg cgagcgtgaa cggcgggatc
     1261 gtgtcgcgca ccgcgcgccc gacgatcgag ttgccggccc acagcgccat cacgacgcac
     1321 agcatcgcgt acgcggtcgc ggtgccggcg cggcgggagg acggggacgg gccttgcatc
     1381 cgcacgattc tggcgaactg ccgcggccgg cgcggacgtg gggccaagtc gcgccccgtt
     1441 tgcgcacccc ggaatcgccc gcggcgcgca ggtccgccgg gcacgcgcgg cggcgttctc
     1501 gcgcgacaat ggcgcccggc gcggccggca cggctcgcgg gggaggatgc gatgttcatc
     1561 atgttcggat ggcgcaagga gacgcgcgag ctgcccggcg acctgcgctg ccactgctac
     1621 cggtgccagc ggccgcgcgg gtgggagcac tggaaggaga ccgagtgggt ctcgttcttc
     1681 atgatcaaga cgattccgtt cctgtcccgc tcgcacgtgg tctgcgcggc ctgccgcgag
     1741 agcttcgcga tcgacggccg gcgggcgcgg gaccttgcgg ccggccgcgg cctcccggcg
     1801 ctcgcggcac acgtggaagg gctgcaactg gccggcaaga ccgaggtgca gcgccgcttc
     1861 ctgctggccc agcgggagca ccatccgccg cagtgaagcg cctcggcggc ggcgcgcgcc
     1921 cggcggcggt gcgaggcatc atcgccccat ggacgagacc tacgtcagca ccgacgtcga
     1981 gaccgacggc cccatccccg gcccgcattc catgctgagc ctcggctcgg ccgcgtacac
     2041 ggcggacaag cgcctgctgg ggaccttctc cgccaatttg gagacgctgc cgggcgcgag
     2101 cccccacccg cagaccgccg cgtggtgggc gacgcagccc gaggcctggg ccgcgtgccg
     2161 gcgcgacctc gaggcgcccg aggccgcgtt gcggcgctac gctgcctgga tcgcgacgct
     2221 gccgggcagg cccgtcttcg tcgcctatcc ggcgggcttc gacttcctgt tcgtctactg
     2281 gtacctgatc cgcttcaccg gcgcgagtcc gttcagccac tcggcgctgg acatgaagac
     2341 cttcgcgatg gccgtgctcg gcaccggcta ccgcgagagc acgaagaacc gcatgccccg
     2401 ggattggttc gacccgctgc cgcatacgca tgtcgcgctg gacgacgcga tcgagcaggg
     2461 cgccctgttc tgcaacatgc tggccgcgag caggaagccc gcggcgtgaa ggcggcgcga
     2521 gacctcagcc gatgcgtccg agcaggaggt actccatcag cgccttctga acatgcatgc
     2581 ggttctccgc ctcgtcccag acgaccgact gcggcccgtc gatgacgtcg gcggtgacct
     2641 cctcgccgcg atgggcgggc aggcagtgca tgaacagggc gtccgggcgc gcgaccgcca
     2701 tcatgccggc gtccacgcgc cattgcgcga aggcctccat gcgcttggcg ttctcggcct
     2761 cgtagcccat gctggtccag acgtcggtcg tcaccaggtc ggcgtcgcgg caggcgtcca
     2821 gcggatggtc gaacaccttg acgcagtcgg cgcggcgcac gccggccacc tcggcgtcga
     2881 tctcgtagcc gctgggcgtg ctcacgtgca gcgtgaagcc gaggatgtcg gccgcctgca
     2941 gccaggtgtt ggccatgttg ttgccgtcgc ccacccaggc caccgtcttg ccggcgatcg
     3001 cgccgcggtg ctcgatgaag gtgaacaggt cggcgaggat ctggcaggga tggaactcgt
     3061 tggtcaggcc gttgatgacc ggcacgcgcg aatgcgcagc gaagcgttcg atcttggtct
     3121 gctcgaaggt gcggatcatc accaggtcga ccatgcggct gatgacgcgc gccgagtcct
     3181 cgatcggctc cgcgcggccc agctggctgt cgccggtggt caggttcacg acggagccgc
     3241 ccatctggta catgccggcc tcgaagctca cgcgcgtgcg cgtcgaggcc ttctcgaaga
     3301 tcatcgccag cgtgcggtcg accagcggct ggtacttctc gtagttcttg aagcgcttct
     3361 tgatctcgcg cgtgcgctcg aagaggtagg cgtattcctc ggcgcgcagg tctttgaact
     3421 gcaggtagtg cttcatcagg ctgttcccgg gcttcatgcc tgctccgcca ggaaggcctt
     3481 caccaccggc gcgaggcggg cgacgatctc gtcggcctcg gccgtcgtca ggatgagcgc
     3541 cggcacgatg cggatgacgc tgtcggcggt gacgctgatc agcaggcccg cctcggccgc
     3601 gcgcgtgagc accgcgccgc acggccgcgc cagctcgacg ccgatcatca ggccttcgcc
     3661 gcgcacttcc ttcacgccgg gcaggccgcc cagctcgcgc tgcagcgcgg ccttcaggtg
     3721 cgcgccgacg cgcgcggcgt tgtcgatgag gccctcggac tcgatcaggc gcagcgtctc
     3781 gaccgcggcg cgcatcgcga gcgggttgcc gccgaaggtc gtgccgtggt tgccgggctg
     3841 cagcacgtcc ttggcgcgct cgccggcgag gaaggcgccg accggcacgc ccgagcccag
     3901 ccccttggcg agcgacatcg cgtccggcac gatgcccgac cactggaagc cgaaccactt
     3961 gccggtgcgc gcgatgccgc actggatctc gtcgagcatc agcagccagc cgcgctcgtc
     4021 gcagatcgcg cgcagctcgc gcaggtactc gtggcgcgcg gggttgatgc cgccttcgcc
     4081 ctgcaccggc tcgagcatca ccgcgacgac gtcggggttc gactcggcga cggcgcgcac
     4141 ggcggcggcg tcgttgaagg gcacgcgcac gaagccctcg accagcgggc cgaagccttc
     4201 ctggatcttc gggttgccgg tggccgacag cgtggcgagc gatcggccgt ggaaggcacg
     4261 ctcgcacacg acgatctcgg gccgcgcgac ccccttgcca tggccgtact tgcgcgccag
     4321 cttgatcagg ccttcgttgg cctccaggcc ggtggagcag aagaacgcgg ccgccaggcc
     4381 cgaccgctcg cagagcatcg cggcgagcgt ctcctgcagc ggcacctggt aatagttcga
     4441 gctgtggatg atcttgccgg cctggtcggc gatcgccgcg accagttgcg gatgggcatg
     4501 gcccagcgtg ttgacggcga tgccggccag cccgtcgaga tactcgcggc cctccgtgtc
     4561 ccagacgcgg cagccgcggc cgtgcgacaa cgcgaacggc aggcggccgt aggtgttcat
     4621 cacgtggggc atcgcggtgg gcgtggacat cggggggtgc tcctgcacag gtcggggacg
     4681 gagtggatgg ccgggtggcg cggcg
//
LOCUS       DASVLN010000469         8638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_509223, whole genome shotgun sequence.
ACCESSION   DASVLN010000469 DASVLN010000000
VERSION     DASVLN010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509223"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1949)
                     /locus_tag="VF453_11575"
     CDS             complement(231..1949)
                     /locus_tag="VF453_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7688342.1"
                     /translation="MSSIPVRSGGRVPTKPAGITGDERKVIFASSLGTVFEWYDFFVY
                     GSLAAYVNKAFFTGLDPTAGYIATLLVFAAGFFVRPFGALVFGRLGDMIGRKYTFLVT
                     ILIMGLSTAVIGVIPTYAQIGGAAPVLLILLRVLQGLALGGEYGGAATYVAEHSPHGK
                     RGEYTSWIQTTATLGLLLSLVAILGFKGMVTKEAFEDPWGWWRAPFIASIVLLAISVW
                     IRLSMNESPAFKRMKEEGRTSKAPLTESFTKWGNLKIVLLALFGLVAGQGVVWYAGQF
                     YAMIFMTNSLKIDATTVQWMIATALVLGTPFFVVFGRLSDRIGRKWIILGGMALAVAT
                     YFPIFKGLAAVGNPELTQAQATAQITVKADPASCAFQGSPVAREIDFLSSCDIAKRAL
                     TANSASYDNEAVAPGTPATVTIGGTTLTAPSGMLAAGGFKFDDASGKAIAAFKKDVAA
                     AMKAAGYPAKAKPIAAFSGQWWEMVGLLWILMIYVTMVYGPIAAMLVELFPTRIRYTS
                     MSLPYHIGNGWFGGLLPSITLAMVAQNGDMFHGLWYPVGIAAASAVVGALFIRETKDT
                     DIYARD"
     gene            2320..3228
                     /locus_tag="VF453_11580"
     CDS             2320..3228
                     /locus_tag="VF453_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688343.1"
                     /translation="MPRALGIQDGTLALARSPVPGVTDVARRAVGGWSRTDVLLEVIH
                     RTMKLVPGQVAGKLADLTTPTSLSFLAGSLVLWVAAHAFGPGEVMDLLVLGAAMLFLG
                     HEAFDFGQHMALFVSKTLHATDERDIDDAAHHLASAIAIVGIDILLVMLTRGGTKAWN
                     NRYRPTIKADPTMPAGEGLTFKYGDIEYSSAGTQTQQQLALYHEMVHSFLSPKLRAFR
                     TLRANLGMFGYRKSEFLRYLEEALAESYAQLRVHGIKGLPVGIRFPVVNGYVRLGGVV
                     KEAVIAAGAVVVLIGGLRYVVSLEES"
     gene            3225..3458
                     /locus_tag="VF453_11585"
     CDS             3225..3458
                     /locus_tag="VF453_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX7688344.1"
                     /translation="MSRPPISIEDARRIAGDLARQRGWPFLEPVDVHLRRPWLFGTAR
                     WRVFSNAECRGQNVAVEIDAETGDILSCGYGMR"
     gene            complement(3463..4053)
                     /locus_tag="VF453_11590"
     CDS             complement(3463..4053)
                     /locus_tag="VF453_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX7688345.1"
                     /translation="MQRVIFDLDVVSPYAYLAFERLPQALEGLSHHVEYRPLLFAGLL
                     KAWGHTAPVDVAPKRAWLFRQCAWIAARDGVPFAPPANHPFNPLALLRLMIASVPAGQ
                     HPNRRVVELAFRHAWARDGGDPNEPGALARLVEAVAPVRDPQGDEVKAELRARTEDAV
                     RAGVFGVPTFRLDDGQAFWGQDSMEMLADAMRRGGA"
     gene            complement(4077..4331)
                     /locus_tag="VF453_11595"
     CDS             complement(4077..4331)
                     /locus_tag="VF453_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEX7688346.1"
                     /translation="MADPVLRDDPAGAPSTPEFEARLASALASSPVPSPCVNICRMDA
                     ANRLCLGCRRSLDEIAAWSRLDDAAKRAVWRQLPARRPAG"
     gene            complement(4337..4837)
                     /locus_tag="VF453_11600"
     CDS             complement(4337..4837)
                     /locus_tag="VF453_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX7688347.1"
                     /translation="MTTVSDRPEGFQRVERELARLGHPHAPQFLDVAARTSQEAADAL
                     GVQVGQIAKSVVFRRKADEVAVLVVASGDRRVDERKVAGIVGPIGRADADFVKARTGF
                     TIGGVSPLAHATPVVVLVDRELFRFDRVWAAAGHPNGVFPMTPDELAALTGAPVHDVV
                     QAPPAA"
     gene            complement(4834..5763)
                     /locus_tag="VF453_11605"
     CDS             complement(4834..5763)
                     /locus_tag="VF453_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX7688348.1"
                     /translation="MSLPTHVKIVDVGPRDGLQNESAPVPAEAKIGLVRRLQDAGLRE
                     IEVTSFVSPKWVPQMADAAEVMAGIERRRGVRYSVLTPNLKGWEAAAATRPDEIVVFG
                     AASEAFSQKNINCGIAESIERFAPVVAAARAAGVKVRGAISCAVGCPYEGEVSPERVG
                     MVARLMHGIGVQHVGVADTIGVGTPLKVRRAMEAALAVYPIEEVSGHFHDTYGQALSN
                     IYECLLMGVSTFDCSVSGLGGCPYAKGATGNVATEDVVYLLHGLGIATGIDLDRLVDA
                     GAWIRERIGKPNGSRVARALLAKRGVAVAAQGE"
     gene            complement(5773..7794)
                     /locus_tag="VF453_11610"
     CDS             complement(5773..7794)
                     /locus_tag="VF453_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX7688349.1"
                     /translation="MFDKILIANRGEIACRVAATARRLGVKTVAVYSDADARARHVAA
                     CDEAVRIGEPAPRDSYLRWERIIEAAKATGAQAIHPGYGFLSENEDFAQACADAGLAF
                     IGPPPSAIRAMGLKAESKQLMEKAGVPLVPGYHGADQDPALLRREADRIGYPVLIKAS
                     AGGGGKGMRAVSAAEEFDAALASCKREAINSFGDDAVLVERYVTRPRHIEIQVFFDGH
                     GNGVYLFERDCSVQRRHQKVLEEAPAPGMTPERRREMGEAAVAAARAVGYVGAGTVEF
                     IAEQDGRFYFMEMNTRLQVEHPVTEAITGHDLVEWQLRVASGERLPALQGELAIRGHA
                     IEARICAENPEAGFLPATGTLSVLRWPAHVEFRRNESQDGARPPAAVRVDTGVDEGDA
                     ISPHYDSMIAKLIVWGEDRAQALARLDAALAQTHVAGVVNNVAFLRRVARTPSFANAD
                     LDTALIERERARVFGQGELSRVEVAAGVVARALALQHAGVDADPWSRRDGWRMHGVSV
                     RRFELEWQGEPLDVQLRARHDGGALLAVAGTEFPFASRPLGDERFEVVLGAGEALRRL
                     TLSVYQLGEQVSVFSVAGSAQATLVDALAHAGEGHQEAGRLTAPMPGKVVAYLVGKGD
                     AVKTGQPLAVLEAMKMEHTIAAPRDGTVAELLFAPGDQVSEGGELLTLD"
     gene            complement(7820..8599)
                     /locus_tag="VF453_11615"
     CDS             complement(7820..8599)
                     /locus_tag="VF453_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7688350.1"
                     /translation="MTATLQIRRDGAVARVFLDRPEVRNAFNDGVIAELTAAFAGFAA
                     DATLRAVVLGGHGKAFCAGADLAWMRAMADYTWEQNRADAQGLADMLHAIWSCPVPVV
                     GRIHGDAYAGGVGLAAVCDVLVAAEGVNFCLSEAKLGLLPATIGPYVVRALGEQASRR
                     YFTTAERFDAARARELGFVHEVVPADALDEKVDEIVAALVANGPAAVRACKRLVQDVA
                     HRAIDAELRADTARRIADIRASAEGKEGVQAFLNKRDPSWR"
BASE COUNT         1261 a         3068 c         3083 g         1226 t
ORIGIN      
        1 gcctcctggc acgccgcgct cccgcccccg ccgcacacct ccgcgcggac gccgccggcg
       61 gtcgctgtgc cgccggcggt aggccggaaa accattgtgg caccgccccg tgccgggagc
      121 cgagcgtgca caccatcggt aacaagacct tgctcaggac cgcacgcgca ggccggcgcc
      181 caacgaaaaa cggccccgcg gggccgttcg tggaagggtg cggcgcgcga tcagtcccgt
      241 gcgtagatgt cggtgtcctt cgtctcgcgg atgaacaggg cgccgacgac ggcgctcgcc
      301 gccgcgatgc cgaccgggta ccacaggccg tggaacatgt cgccgttctg cgcgaccatc
      361 gccagcgtga tcgagggcag caggccgccg aaccagccgt tgccgatgtg gtagggcagg
      421 ctcatcgagg tgtagcggat gcgggtcggg aacagctcga ccagcatggc ggcgatcggg
      481 ccgtagacca tcgtcacgta gatcatgagg atccacagca ggcccaccat ctcccaccac
      541 tggccgctga atgccgcgat cggcttcgcc ttggcagggt agccggcggc cttcatcgcc
      601 gcggcgacat ccttcttgaa cgccgcgatc gccttgcccg aggcgtcgtc gaacttgaag
      661 ccgccggcgg ccagcatgcc gctcggagcc gtcagcgtgg tgccgccgat cgtcaccgtg
      721 gccggcgtgc caggcgcgac ggcctcgttg tcgtagctcg ccgagttggc ggtcagcgcg
      781 cgcttggcga tgtcgcacga ggacaggaag tcgatctcgc gcgccaccgg gctgccctgg
      841 aaggcgcagc tggcgggatc ggccttgacc gtgatctgcg cggtggcctg cgcctgcgtc
      901 agctcgggat tgccgaccgc ggccaggccc ttgaagatcg ggaagtaggt cgccacggcc
      961 agcgccatgc cgccgaggat gatccacttg cggccgatgc ggtcggacag gcggccgaac
     1021 acgacgaaga acggcgtgcc cagcaccagc gcggtggcga tcatccactg caccgtcgtc
     1081 gcgtcgatct tgagcgagtt ggtcatgaag atcatcgcgt agaactggcc cgcgtaccac
     1141 acgacgccct ggcccgcgac gaggccgaag agcgccagca gcacgatctt caggttgccc
     1201 cacttcgtga acgactcggt cagcggcgcc ttggacgtgc gaccttcctc cttcatgcgc
     1261 ttgaaggcgg gcgactcgtt catcgacagg cggatccaca cgctgatggc cagcaggacg
     1321 atcgatgcga tgaacggcgc gcgccaccag ccccaaggat cctcgaaggc ttccttggtg
     1381 accatgccct tgaagcccag gatcgcgacc agcgacagca gcaggccgag cgtggccgtg
     1441 gtctggatcc acgacgtgta ctcgccacgc ttgccgtgcg gcgagtgctc ggcgacgtag
     1501 gtcgcggccc ccccgtactc gccgccgagc gccaggccct gcagcacccg cagcaggatc
     1561 agcaggacgg gcgccgcgcc gccgatctgg gcgtaggtcg ggatgacgcc gatcacggcc
     1621 gtcgacaggc ccatgatcag gatcgtgacc aggaacgtgt acttgcgccc gatcatgtcg
     1681 cccagccggc cgaagaccag cgcgccgaac ggccgcacga agaagccggc cgcgaacacg
     1741 agcagcgtcg cgatgtagcc ggcagtgggg tcgaggcccg tgaagaaggc cttgttgacg
     1801 taggcggcga gcgagccgta gacgaagaag tcgtaccact cgaatacggt gccgagcgat
     1861 gaggcgaaga tcaccttgcg ctcgtcgccg gtgatgcccg cgggtttcgt gggcacgcgg
     1921 ccgccgctgc ggacggggat gctggacatg gcgtgttgtc tcctgtgatg catgacgcct
     1981 ccgccgcacg cggccgatgc gaagcgcctg tcgcacatcg tcgaagcgcg ctctgaccca
     2041 cgtctgacgc ggcgcttata cgagacttac aacccgaggg ataacgcgga gagttcgttt
     2101 ctcatcgtgg gatggaaccc tggacacggg taaacccggc ggcgcgccgc cgaggttgcc
     2161 ggacgcagat ggcgtcgagc gaccacgacc cggggaatgt gccggcaacc gccccccttg
     2221 cgggggtggc agcaagcggt gtccgttggc atcctgctgc gttccgcacg acgcaccgcg
     2281 acaggaatgg cgaattcaag aacaccgggt ccgcaaggag tgccccgcgc cctcggcatc
     2341 caggacggca ccctggcact cgcacgatca cctgtcccgg gcgtgacgga tgtcgcgcgc
     2401 cgcgccgtcg gtggatggag ccgcaccgac gtactgctcg aagtgatcca tcgcacgatg
     2461 aagctggtgc cggggcaggt cgccggcaag ctagcggacc tgaccacgcc gacgtcgctc
     2521 tccttcctcg ccggatcgct agtgctgtgg gtcgcggcgc atgccttcgg gccgggcgag
     2581 gtgatggacc tgctggtact cggggccgcg atgctgttcc tcggacacga agcgttcgac
     2641 ttcggccagc atatggccct cttcgtgtcg aagacgctcc acgcgaccga cgagcgcgac
     2701 atcgacgatg ctgcgcacca cctcgcgtcc gccatcgcca tcgtgggcat cgacatcctg
     2761 ctggttatgc tgacccgcgg cggcaccaag gcctggaaca atcggtaccg gcccacgatc
     2821 aaggctgatc ccaccatgcc ggcgggcgag ggtctgacct tcaagtatgg cgatatcgag
     2881 tactcgtccg cgggcaccca gacgcagcag cagctcgcgc tttaccacga gatggtgcat
     2941 tcgttcctgt ccccgaagct gcgggcgttc aggaccttga gggccaacct cggcatgttc
     3001 ggctacagaa agtcggaatt cctccgctat ctcgaagaag ccctcgccga gagctacgcg
     3061 caactccgcg tgcacggtat caaggggctg ccggtcggca taagattccc ggtcgtcaat
     3121 ggatacgtgc gactcggcgg cgtcgtaaag gaggctgtga tcgccgcggg cgccgtcgtc
     3181 gtcctgatcg gcggcttgcg gtacgtcgtc agcctggagg aatcgtgagc cgtcccccca
     3241 tctcgatcga ggatgcgcgc cgcatcgccg gcgatcttgc acggcaacgt ggctggccct
     3301 tcctcgaacc ggtcgacgtg cacctgcgca ggccgtggct gttcggcacg gcgcgctgga
     3361 gggtgttctc gaacgccgag tgccgaggcc agaacgtcgc cgtcgagatc gacgccgaga
     3421 ccggtgacat cctgtcctgc ggctacggca tgcgctgagc gctcaggcgc cgccccgccg
     3481 catcgcgtcg gccagcatct ccatcgaatc ctggccccag aaggcctggc cgtcgtcgag
     3541 gcggaaggtc ggcacgccga agacgccggc gcgcacggcg tcctcggtgc gggcgcgcag
     3601 ttcggccttg acctcgtcgc cctgcggatc gcgcaccggc gcgacggcct cgacgaggcg
     3661 ggcgagcgca ccgggttcgt tgggatcgcc gccgtcgcgg gcccaggcat ggcggaaggc
     3721 cagttcgacg acgcgccggt tcgggtgctg gccggcgggc acgctggcga tcatcagccg
     3781 cagcagcgcg agggggttga acggatggtt ggccggcggc gcgaagggca ccccgtcgcg
     3841 cgccgcgatc caggcgcact ggcggaacag ccacgcgcgc ttgggcgcga cgtccaccgg
     3901 cgccgtgtgg ccccaggcct tgagcaggcc ggcgaacagc agcggcctgt actcgacatg
     3961 gtgcgacagc ccctccagcg cctgcggcag gcgctcgaag gcgaggtagg cgtagggcga
     4021 gacgacatcg aggtcgaaga tcacgcgctg catcgcgggc tcctttcgcg aagggctcag
     4081 ccggccgggc ggcgcgcggg cagctgccgc cagaccgcgc gcttggcggc gtcgtcgagg
     4141 cggctccagg cggcgatctc gtcgagcgag cgccggcagc cgaggcacag gcggttggcc
     4201 gcatccatcc ggcagatgtt gacgcacggc gagggcacgg gcgagctcgc gagcgcgctc
     4261 gcgaggcgcg cctcgaactc gggcgtcgac ggcgcgccgg cggggtcgtc gcgcaacacg
     4321 ggatcggcca tggcgctcac gccgcgggcg gcgcctgcac gacgtcgtgc acgggcgcgc
     4381 cggtcagggc ggcgagttcg tcgggcgtca tcgggaacac gccgttcgga tggccggccg
     4441 cggcccagac gcggtcgaag cggaacagct cccggtcgac cagcacgacc accggcgtcg
     4501 catgcgcgag cggcgagacg ccgccgatcg tgaagccggt gcgcgccttg acgaagtcgg
     4561 cgtcggcgcg gccgatcggg ccgacgatgc cggccacctt cctttcgtcg acccggcggt
     4621 cgcccgaggc gacgaccagg accgcgacct cgtcggcctt gcggcggaac acgacgctct
     4681 tggcgatctg gccgacctgc acgcccagcg catcggccgc ctcctgcgag gtgcgcgccg
     4741 cgacgtcgag gaactgcggc gcgtgcggat ggccgaggcg cgcgagctcg cgctcgacgc
     4801 gctggaagcc ttcggggcgg tcggacaccg tcgtcattcg ccctgcgcgg ccaccgccac
     4861 gccccgcttg gccagcagcg cccgcgcgac gcgcgagccg ttcggcttgc cgatgcgctc
     4921 gcggatccac gcgcctgcgt cgaccagcct gtcgaggtcg atgccggtgg cgatgcccag
     4981 gccatgcagc aggtagacga cgtcctcggt ggcgacgttg ccggtcgcgc ccttcgcgta
     5041 cgggcagccg ccgaggcccg agacgctgca gtcgaaggtc gagacgccca tcagcaggca
     5101 ctcgtagatg ttggacagcg cctggccgta ggtgtcgtgg aaatggccgc tgacttcctc
     5161 gatcgggtag accgccagcg ccgcctccat cgcgcggcgc accttcagcg gcgtgccgac
     5221 gccgatcgtg tccgcgacgc cgacgtgctg cacgccgatc ccgtgcatca gccgcgcgac
     5281 catgccgacg cgctcgggcg acacctcgcc ctcgtacggg cagccgaccg cgcaggagat
     5341 cgcgccgcgc accttcaccc ccgcggcgcg cgccgccgcg acgacggggg cgaagcgctc
     5401 gatcgactcg gcgatcccgc agttgatgtt cttctggctg aacgcctcgc tcgcggcgcc
     5461 gaagacgacg atctcgtcgg gacgcgtcgc ggccgcggcc tcccagccct tgaggttggg
     5521 cgtgagcacc gagtagcgca ccccgcggcg ccgctcgatc ccggccatca cctcggccgc
     5581 gtcggccatc tgcggcaccc acttcgggct gacgaagctc gtgacctcga tctcgcgcag
     5641 gccggcgtcc tgcaggcgcc ggacgagtcc gatcttggcc tcggcgggca cgggcgccga
     5701 ctcgttctgc aggccgtcgc gcgggccgac gtcgacgatc ttcacgtggg tgggcaggga
     5761 catcgcgggc attcagtcga gagtgaggag ctcgccgcct tcggagacct ggtcgccggg
     5821 cgcgaacagc agctcggcca cggtgccgtc gcgcggggcg gcgatggtgt gctccatttt
     5881 catcgcttcg agcaccgcca gcggctgtcc ggtcttcacg gcgtcgccct tgccgacgag
     5941 gtaggccacg accttgccgg gcatcggcgc ggtcaggcga ccggcctcct ggtggccttc
     6001 gccggcatgg gcgagggcgt ccacgagcgt cgcctgcgcg ctgccggcga ccgagaagac
     6061 cgagacctgc tcgccgagct ggtacaccga cagcgtgagc cggcgcagcg cctcgccggc
     6121 cccgagcacg acttcgaagc gttcgtcgcc gagcggtcgc gaagcgaacg ggaactcggt
     6181 gccggcgacc gccagcagcg cgccgccgtc atggcgcgcg cgcagttgca cgtcgagcgg
     6241 ctcgccctgc cattcgagct cgaagcgccg caccgagacg ccgtgcatgc gccagccgtc
     6301 gcgccgcgac cacgggtcgg catcgacgcc cgcatgctgc agcgcgagtg cgcgcgcgac
     6361 gacgccggcc gccacctcga cgcgcgaaag ctcgccctgg ccgaacaccc gcgcgcgctc
     6421 gcgctcgatc agcgcggtgt cgagatccgc gttcgcgaac gacggcgtgc gcgcgacgcg
     6481 gcgcaggaag gcgacgttgt tgaccacgcc cgcgacgtgc gtctgcgcca gtgccgcatc
     6541 gagccgcgcg agcgcctgcg cgcgatcctc gccccagacg atcagcttgg cgatcatgga
     6601 gtcgtagtgc ggcgagatcg cgtcgccctc gtccacgccc gtgtccacgc gcaccgcggc
     6661 cggcgggcgc gcgccgtcct gcgactcgtt gcggcggaac tcgacgtgcg ccggccagcg
     6721 cagcaccgac agcgtgccgg tggcgggcag gaagccggcc tcggggttct cggcgcagat
     6781 gcgcgcctcg atcgcgtggc cgcggatcgc gagctcgccc tgcagcgccg gcaggcgctc
     6841 gcccgaggcg acgcgcagtt gccactcgac caggtcgtgg ccggtgatcg cctcggtcac
     6901 cgggtgctcg acctgcagcc gcgtgttcat ctccatgaag tagaagcggc cgtcctgctc
     6961 ggcgatgaac tccaccgtcc cggcgccgac gtagcccacc gcgcgcgcgg cggccaccgc
     7021 ggcctcgccc atctcgcgcc ggcgctcggg cgtcatgccc ggtgcgggcg cttcctcgag
     7081 caccttctgg tggcgccgct gcaccgagca gtcgcgctcg aacaggtaga cgccgttgcc
     7141 gtggccgtcg aagaagacct ggatctcgat gtggcgcggg cgcgtgacgt agcgctcgac
     7201 gagcacggcg tcgtcgccga acgagttgat cgcctcgcgc ttgcacgacg cgagcgcggc
     7261 gtcgaactcc tcggccgccg acaccgcgcg catgcccttg ccgccgccgc cggcgctcgc
     7321 cttgatcagc accgggtagc cgatccggtc ggcctcgcgc ctgagcagcg cggggtcctg
     7381 gtcggccccg tggtagccgg gcaccagcgg gacgccggcc ttctccatca gctgcttcga
     7441 ttcggccttc aggcccatcg cgcggatcgc cgacggcggc gggccgatga aggcgaggcc
     7501 cgcgtccgcg caggcctgcg cgaagtcctc gttctcgctc aggaagccgt agcccgggtg
     7561 gatcgcctgc gcgccggtgg ccttcgcggc ctcgatgatg cgctcccagc gcaggtagct
     7621 gtcgcgcggc gcgggctcgc cgatgcgcac cgcctcgtcg caggccgcga cgtggcgcgc
     7681 gcgggcgtcc gcgtcggagt agacggccac ggtcttcacg ccgaggcggc gggcggtggc
     7741 ggcgacgcgg caggcgatct cgccgcggtt ggcgatcagg atcttgtcga acatcaggct
     7801 ctttctgctt gctggattct cagcgccagg acgggtcgcg cttgttgagg aaggcctgca
     7861 cgccctcctt gccctcggcg ctggcgcgga tgtcggcgat acgtcgtgcc gtatcggcgc
     7921 gcagctcggc gtcgatcgcg cggtgggcga cgtcctgcac gaggcgcttg caggcgcgca
     7981 ccgcggccgg gccgttggcc acgagcgcgg cgacgatctc gtcgaccttc tcgtcgagtg
     8041 cgtcggccgg caccacctcg tgcacgaagc cgagctcgcg ggcgcgtgcg gcgtcgaagc
     8101 gctcggcggt cgtgaaatag cggcgcgagg cctgctcgcc gagcgcgcgc acgacgtacg
     8161 ggccgatcgt cgcgggcagc aggccgagct tcgcttcgct caggcagaag ttcacgccct
     8221 ccgcggcgac cagcacgtcg cacaccgccg cgaggccgac gccgccggcg tacgcgtcgc
     8281 cgtggatgcg gccgaccacc ggcaccgggc acgaccagat cgcgtgcagc atgtccgcga
     8341 ggccctgggc gtcggcgcgg ttctgctccc aggtgtagtc ggccatcgcg cgcatccacg
     8401 cgaggtcggc gccggcgcag aacgccttgc cgtggccgcc cagcaccacc gcgcgcagcg
     8461 tggcgtcggc ggcgaacccc gcgaaggcgg ccgtcagctc ggcgatcacg ccgtcgttga
     8521 acgcgttgcg cacctcgggg cggtcgagga agacgcgggc gacggcaccg tcgcggcgga
     8581 tctgcaaggt ggcggtcatg gagcccttca gttggacggc gccggcgcgg gcaccggc
//
LOCUS       DASVLN010000470         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_293433, whole genome shotgun sequence.
ACCESSION   DASVLN010000470 DASVLN010000000
VERSION     DASVLN010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293433"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VF453_11620"
     CDS             <1..565
                     /locus_tag="VF453_11620"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7688351.1"
                     /translation="IWSGYVGVADVDAAAADVIGAGGKLLRPVAAIPGVGRFAVAADP
                     QGASFMIFQPEPGDPPVMPAAGAPGSIGWHELHAVDWSSALDFYRRLFGWTKGETMDM
                     GPNGVYQLFEIDGVPAGAVLNKAPDRPAPGWDFYFNVDAIDAAVERLRAAGGELLRGP
                     HQVPGGSWIAIARDPQGACFSLVAAKR"
     gene            592..>1512
                     /locus_tag="VF453_11625"
     CDS             592..>1512
                     /locus_tag="VF453_11625"
                     /inference="COORDINATES: protein motif:HMM:NF008607.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX7688352.1"
                     /translation="MNLPAPPRAPLKVGLVGYGYAGRTFHAPLIAAVPGLALTAIASS
                     DAAKVHVDWPDVAVHAKPQELLTRTDLDLVVIATPNETHHPLARAALHAGLHVVVDKP
                     FTITLGEGRELAALARERGRVLSVFHNRRWDGDFLTLRRLVREQSLGRVVEVSSRWQR
                     FRPQVQARWREGAAPGAGLWYDLGPHLVDQALQLFGPPEGLQLVHDRTRDGAIADDWF
                     HARLRYDRLHVDLHASMLAAASEPRFVVHGTAASFVKHGLDTQEDALKRGVRPTWPAA
                     AGWGRDPGPATLVTRDPDGRAQRVEVALAPG"
BASE COUNT          179 a          589 c          549 g          195 t
ORIGIN      
        1 gatctggtcg ggctacgtcg gcgtcgcgga cgtcgatgcc gcggccgccg acgtgatcgg
       61 cgccggcggc aagctgctgc ggccggtggc ggcgatcccg ggcgtcggcc gcttcgccgt
      121 cgccgccgac ccgcagggcg cgagcttcat gatcttccag cccgagcccg gcgatccgcc
      181 ggtcatgccg gccgcgggcg cgccgggctc gatcggctgg cacgagctgc acgcggtcga
      241 ctggtcctcc gcgctcgact tctaccgtcg cctgttcggc tggacgaagg gcgagacgat
      301 ggacatgggc ccgaacggcg tctaccagct gttcgagatc gacggcgtgc cggcgggcgc
      361 cgtcctgaac aaggcgcccg accggcccgc gccgggctgg gacttctact tcaacgtcga
      421 tgcgatcgac gccgccgtcg agcgcctgcg cgccgccggc ggcgagctgc tgaggggccc
      481 gcaccaggtg cccggcggca gctggatcgc gatcgcgcgc gacccgcagg gcgcatgctt
      541 ctcgctggtg gcggccaagc gatgagcgca gcccatccga caggcttccc gatgaacctg
      601 cccgccccgc cgcgcgcccc gctgaaggtc ggcctcgtcg gctacggcta cgccggccgc
      661 accttccacg cgccgctgat cgccgccgtg cccgggctcg cgctgaccgc gatcgcgagc
      721 tccgacgccg ccaaggtcca cgtcgactgg cccgacgtcg ccgtgcacgc gaagccgcag
      781 gagctcctca ctcgcacgga cctcgatctc gtcgtcatcg cgacgcccaa cgagacccac
      841 cacccgctcg cgcgcgcggc gctgcacgcg gggctgcacg tcgtggtgga caagccgttc
      901 acgatcacgc tcggcgaggg ccgggagctc gccgcgctcg cgcgcgagcg cgggcgcgtg
      961 ctctcggtgt tccacaaccg gcgctgggac ggcgacttcc tcaccctgcg ccggctcgtg
     1021 cgggagcagt cgctgggccg cgtcgtcgag gtgagctcgc gctggcagcg cttccgtccg
     1081 caggtgcagg cgcgctggcg cgagggcgcg gcacccggcg ccgggctgtg gtacgacctc
     1141 gggccgcacc tggtcgacca ggcgctgcag ctgttcgggc cgcccgaggg gctgcagctg
     1201 gtgcacgacc gcacccgcga cggcgccatc gccgatgact ggttccacgc ccgcctgcgc
     1261 tacgaccgcc tgcacgtcga cctgcacgcg agcatgctgg ccgcggcgtc ggagcctcgc
     1321 ttcgtcgtcc acggcaccgc ggcgagcttc gtcaagcacg gcctcgacac gcaggaggac
     1381 gcgctcaagc gcggcgtacg cccgacctgg cccgccgccg ccggctgggg ccgggacccg
     1441 ggcccggcga cgctggtcac gcgcgatccc gacggccgcg cgcagcgcgt ggaggtcgcc
     1501 ctcgcgcccg gc
//
LOCUS       DASVLN010000471         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_293587, whole genome shotgun sequence.
ACCESSION   DASVLN010000471 DASVLN010000000
VERSION     DASVLN010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293587"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VF453_11630"
     CDS             complement(<1..1335)
                     /locus_tag="VF453_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX7688353.1"
                     /translation="MNRPAATALRGGPAPKRNGRNNDLVLAALLVLVVVLFVLPLPTP
                     LLDLLIATNLAASLVLLIVAMYVPSASSLSTFPSLLLFTTLFRLALNIASTKLILLHA
                     NAGHIIDTFGKLIVGNNVVVGGVVFLIIAIVQFVVIAKGAERVAEVAARFALDAMPGK
                     QMSIDADVRAGAMSSAEAQRRRQALEQESALHGAMDGAMKFVKGDAVASIIVALVNIM
                     AGLAIGVLMKDMSVGNALHRYAILTVGDGMVSQIPSLLVSIAAGVVITRVDAGGGDSQ
                     LANQIARQVMAHPRALVIAGLALASFLLVPGFPAWAFALLSGGIVTAGLVARRAHEWR
                     RTPAWISHGETEAPHAVAAPLVVRLASDLRAGVDRHVLDLRLVAAKEAVEGDLGPVFP
                     RLRVEHASEVASFGYEILVEDVPVSRGALRPGWRLLDPAGATVLPAGAEVAEP"
BASE COUNT          248 a          599 c          566 g          215 t
ORIGIN      
        1 cggctcggcc acttccgcgc cggccggcag caccgtcgcg cccgcgggat cgagcaggcg
       61 ccagcctggc cgcagcgccc cgcgcgagac gggcacgtcc tcgacgagga tctcgtagcc
      121 gaaggatgcg acctcgctcg catgctcgac gcgcaggcgc gggaacacgg gccccaggtc
      181 gccctcgacc gcctccttcg cggcgacgag gcgcaggtcg agcacgtggc ggtccacgcc
      241 ggcgcgcagg tcggaggcga gccgcaccac cagcggggcg gcgacggcat gcggcgcctc
      301 ggtctcgccg tgcgagatcc aggccggcgt gcggcgccat tcgtgcgcgc gccgcgccac
      361 gaggcccgcg gtcacgatgc cgcccgacag cagcgcgaac gcccacgccg ggaagcccgg
      421 cacgagcagg aagctcgcga gcgccaggcc tgcgatcacg agcgcgcgcg gatgcgccat
      481 gacctggcgc gcgatctggt tggcgagctg cgagtcgccg ccgccggcgt ccacccgcgt
      541 gatgaccacg cccgccgcga tcgacaccag cagcgacggg atctgcgaca ccatgccgtc
      601 gccgacggtg aggatggcgt agcggtgcag cgcgttgccc acgctcatgt ccttcatcag
      661 gacgccgatc gccaggcccg ccatgatgtt gacgagcgcg acgatgatgc tcgccaccgc
      721 gtcgcccttg acgaacttca tggcaccgtc catcgcgccg tgcagcgcgc tctcctgctc
      781 gagcgcctgg cgccggcgct gcgcctctgc cgaggacatc gcgcccgcgc gcacgtcggc
      841 gtcgatgctc atctgcttgc ccggcatcgc gtcgagcgcg aaccgcgccg ccacctccgc
      901 cacgcgctcc gcgcccttgg cgatcacgac gaactgcacg atcgcgatga tgaggaagac
      961 gacgccgccg acgacgacgt tgttgcccac gatcagcttg ccgaacgtgt cgatgatgtg
     1021 gcccgcgttg gcgtgcagca ggatgagctt ggtcgacgcg atgttgagcg cgaggcggaa
     1081 cagcgtcgtg aacagcagca gcgacgggaa cgtcgacagc gacgacgccg acggcacgta
     1141 catcgcgacg atgagcagca cgaggctcgc ggcgaggttc gtcgcgatca gcaggtcgag
     1201 cagcggcgtg ggcagcggca gcacgaacag cacgaccacg agcaccagca gggccgccag
     1261 cacgaggtcg ttgttgcgcc cgtttcgctt tggcgcaggg ccgccgcgga gggcggtagc
     1321 ggcggggcgg ttcatgtacc tgtgtccgta tgcactgttg gcttgtggct tgtcggtcac
     1381 tctaggttcg cgtccggagc cggacaagca cgagagctcg tagtcacgca aggtccatcg
     1441 aacggcgcct acgattcttc gtactttcca gcggccgccc tgcttggcta ccctcgcggc
     1501 aacgcggccg ccgggtcgcc tccgacgccc cgagccgcca cgccgtgaac cttgccgacc
     1561 cgccctccga ccgcctgccc gccacgcacg gccgcggtcc ggccgccggg ttcacggagc
     1621 tcggcgac
//
LOCUS       DASVLN010000472         3938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_454977, whole genome shotgun sequence.
ACCESSION   DASVLN010000472 DASVLN010000000
VERSION     DASVLN010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454977"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF453_11635"
     CDS             complement(<1..405)
                     /locus_tag="VF453_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX7688354.1"
                     /translation="MFAVVFEVRPDPRQGDAYLGYAKLLRPELEQVDGFVDNVRYRSL
                     TRPGWLLSLSTWRDEKSLVRWRTRAVHHDVQEKGRGGVLRDYRLRVGQLVADTRPPAG
                     HVLAEQRLDETEVGAARALALVDARLDASRLAA"
     gene            complement(524..1612)
                     /locus_tag="VF453_11640"
     CDS             complement(524..1612)
                     /locus_tag="VF453_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7688355.1"
                     /translation="MAQTLAEEPASFEGEALVDGAGNVWLPYDLPHLRAPSTGWTRLS
                     RRREVRHAGRRPFDWRAPARDELHILNGMGVTLGDSIIGMNALAWMKRRHPTLRIHLY
                     RTPHAPVFVERLYELASHIVEPVTYLPRSLETLPDDVVDLSDFLYWPSFASTPMVDFF
                     IRGLGIAADAVPASAKANRWLSRLPLPSIPAPWSTRRYVLLCDQASTPLRTLPDEHAA
                     AMVDRIWDRYGIPVLGFRPIAHPRYHDVSRHSRNLDQYMAWVKGAAAVIGTDSSAIHV
                     AAGFDVPTMAIFVSIDPGLRARDYWHCRALDERTALTDGLHESDDPAVLREVRRIWRA
                     ILVRAELPWPDLAHHRVQASETALAMPA"
     gene            1903..3072
                     /locus_tag="VF453_11645"
     CDS             1903..3072
                     /locus_tag="VF453_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX7688356.1"
                     /translation="MLHTLRLFALLALALAAGSAGADDVSRSHRPAPPKVILDTDFNT
                     ISDDGQALAMLAQLDAAGALDLLGVTVATGNAWLEQETSDALKAVERLGIEHSIGVYE
                     GAKYPLLHDYDAYLDEVATFGPAVDYVGAFSSPPPTSPSDIVPPPDGFATHTRPQAQH
                     AVDFIIEQVHRHPHEVSILEIAPPTNLAMAIRKDPTIVPLIKQIVTMAGQIYVPGNAY
                     DDAAEFNWWADPEAVKIVLRAPIPRVIVPLDLTNNVPLTKPVYEQIANHRPATPITRL
                     FADAYAPFFGSAPPPYPLFIWDTTALAYLVDPTFATATRDLWVDMNTTFDANYGKSLV
                     YESNPKPSIPLLQPSKVVFAFDTARFYAFYVDLLTRPVPVHDDHDHDHAHGGGGR"
     gene            complement(3098..3787)
                     /locus_tag="VF453_11650"
     CDS             complement(3098..3787)
                     /locus_tag="VF453_11650"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX7688357.1"
                     /translation="MAESIHPFHESHRAAMEAAMRQRLELAEPLLRERAQLPDIDGIK
                     REVMDEFGVVLAQMPDVGGAANRMSDFFMRLLGFMAIGRVLRRHGVPVQVIGDIERDA
                     YQAQLLATPEEERLASGRQFMSLENQALVRAQAARSTTPAHRDEFPADFAYDFVEPGP
                     GDDFEFGIDYKACGFCKFAARHGDRELLPNLCGLDFVAYAARGIRLVRTQTLAAGASH
                     CDFRFSRLPPE"
BASE COUNT          597 a         1406 c         1397 g          538 t
ORIGIN      
        1 ggccgcgagc cggctggcgt cgaggcgggc gtcgaccagc gccagcgcgc gcgcagcgcc
       61 gacctcggtc tcgtcgagtc gctgctccgc gagcacgtgg cccgcgggcg ggcgggtgtc
      121 cgcgacgagc tggcccacgc gcagccgata gtcgcgcagc acgccgccgc ggcccttctc
      181 ctgcacgtcg tggtggacgg cgcgcgtgcg ccagcgcacc agcgacttct cgtcgcgcca
      241 ggtcgacagc gacagcagcc acccggggcg cgtgaggctg cggtagcgga cgttgtcgac
      301 gaagccgtcg acctgctcga gctcggggcg cagcagcttg gcatacccca ggtaggcatc
      361 gccctggcgc gggtcgggcc ggacttcgaa gacgacggcg aacatggcgg gcctcctggc
      421 gggtggtcgg agccgtgatt tcagcgcatc gcgtgccgca acgcttcgcc ggcggacgaa
      481 gcgatcccgc gacgcgccgc ccgccgcgcg tcccgccccg gattcacgcg ggcatcgcga
      541 gggcggtctc gctcgcctgg acgcggtgat gcgcgaggtc cggccagggc agttccgcgc
      601 gcacgaggat cgcgcgccag atccggcgaa cctcccgcag caccgcgggg tcgtcgctct
      661 cgtggagtcc gtccgtcagc gccgtgcgct cgtcgagcgc gcgacagtgc cagtagtcgc
      721 gcgcgcgcag gcccggatcg atgctcacga agatggccat cgtcgggacg tcgaagcccg
      781 ccgcgacgtg gatggcggag ctgtcggtgc cgatgacggc ggcggcgccc ttcacccacg
      841 ccatgtactg gtcgaggttg cgcgaatggc gggagacgtc atggtagcgc gggtgcgcga
      901 tcggacggaa gccgagcacc ggaatgccgt agcggtccca gatgcggtcg accatcgcgg
      961 ccgcatgttc gtcgggcagc gttcgcaggg gcgtgctcgc ctggtcgcaa agcaggacgt
     1021 aacggcgcgt cgaccacggc gcgggaatcg agggcagggg caggcgcgac agccagcgat
     1081 tggccttggc cgaggccggc accgcgtccg ccgcgatgcc caggccgcga atgaagaagt
     1141 cgaccatcgg cgtgctggcg aacgagggcc aatacaggaa gtcggacagg tcgacgacgt
     1201 cgtccggcag ggtctccagc gacctcggca ggtaggtcac gggctcgacg atgtggctgg
     1261 cgagttcata gagccgctcg acgaagacgg gcgcatgcgg cgtccggtac aggtggatgc
     1321 gcagggtcgg atgccggcgc ttcatccagg cgagggcgtt catgccgatg atcgagtcgc
     1381 cgagcgtgac ccccatgccg ttgaggatgt gcagctcgtc gcgcgcaggc gcacgccaat
     1441 cgaaggggcg gcgacccgca tggcggacct cgcgccggcg ggacaggcgg gtccatcccg
     1501 tgctgggcgc gcgaaggtgc ggcaggtcgt agggcagcca gacgttgccg gcgccgtcca
     1561 ccagggcttc gccttcgaac gaggccggct cctcggccag agtctgcgcc atcgatgctc
     1621 ccgtgcggtt ctgctctggc ggcaccggcg tgaggcgaat ccccggccgg gccccggagg
     1681 gcttggacgc atggtggaca gccgtcgtcg agcgcagagg cgggaacgcg aaggcggggg
     1741 tcgccgcgcg aagccgccga gcgcgactca cgtcgctcgc cgcgccgacg ctgcgcggcc
     1801 gttgcgcaga caagcccacg tcgcgagcat gccgccgtcc tgcgcgatga tcgcggccgg
     1861 cacgcggcag ccgcattcgc cgcaacacga caggagacat gcatgttgca cacccttcgc
     1921 ctgttcgcgc tgctcgcgct ggccctcgcg gccggatcgg ccggcgccga cgacgtttcg
     1981 cgcagccacc gtccggcgcc gcccaaggtc atcctcgaca ccgacttcaa cacgatcagc
     2041 gacgacggcc aggcgctggc gatgctcgcg cagctcgacg ccgccggcgc gctcgacctg
     2101 ctgggcgtca cggtcgcgac cggcaacgcg tggctcgagc aggagacctc cgacgcgctg
     2161 aaggcggtcg agcggctcgg catcgagcat tcgatcggcg tctacgaggg cgcgaagtac
     2221 ccgctgctgc acgactacga cgcctacctc gacgaggtcg ccaccttcgg cccggcggtc
     2281 gactacgtgg gcgccttctc gagcccgccg ccgacctcgc cgagcgacat cgtcccgccg
     2341 cccgacggct tcgccacgca cacccggccg caggcccagc acgcggtcga cttcatcatc
     2401 gagcaggtgc accgccatcc gcacgaggtc tcgatcctgg agatcgctcc gccgaccaac
     2461 ctcgcgatgg ccatccgcaa ggatccgacc atcgtgccgc tgatcaagca gatcgtcacg
     2521 atggccgggc agatctacgt cccgggcaac gcctacgacg acgccgccga gttcaactgg
     2581 tgggccgatc ccgaggccgt gaagatcgtg ctgcgggcgc cgatcccgag ggtgatcgtg
     2641 ccgctcgacc tgaccaacaa cgtgccgctg accaagccgg tgtacgagca gatcgcgaac
     2701 caccggcccg cgacgccgat caccaggctc ttcgccgatg cctacgcgcc gttcttcggc
     2761 tcggctccgc cgccgtaccc gctgttcatc tgggacacga ccgcgctcgc ctacctcgtc
     2821 gacccgacgt tcgcgaccgc gacgcgcgac ctctgggtcg acatgaacac cacgttcgac
     2881 gccaactacg gcaagtccct cgtgtacgag tcgaacccga agccgtccat cccgctgctg
     2941 cagccctcga aggtggtgtt cgcgttcgac accgcgcgct tctacgcgtt ctacgtcgac
     3001 ctgctgaccc ggccggtgcc ggtgcacgac gaccacgacc acgaccacgc gcacggcggc
     3061 ggcgggcgct gacgccgtcg ccggcaacgg accggcgtca ttccggcggg aggcgcgaga
     3121 acctgaagtc gcagtggctc gcgcctgccg ccagcgtctg cgtccgcacg aggcggatac
     3181 cccgtgccgc ataggcgacg aagtcgagcc cgcagaggtt gggcagcagt tcccggtcgc
     3241 cgtgccgggc cgcgaacttg cagaagccgc acgccttgta gtcgatgccg aactcgaagt
     3301 cgtcgcccgg gccgggctcg acgaagtcgt aggcgaagtc ggccgggaac tcgtcgcgat
     3361 gcgcgggcgt cgtgctgcgc gcggcctggg cgcgcacgag ggcctgattc tcgagggaca
     3421 tgaactggcg tcccgacgcc agccgttcct cctcgggcgt ggcgagcaat tgcgcctggt
     3481 aggcatcgcg ctcgatgtcg ccgatcactt gcaccggcac gccgtggcgc cgcagcacgc
     3541 ggccgatggc catgaagccg agcaggcgca tgaagaagtc gctcatgcgg ttggccgcac
     3601 cgcccacgtc gggcatctgc gcgagcacga cgccgaattc gtccatcacc tcgcgcttga
     3661 tgccgtcgat gtcgggcagc tgcgcgcgct cgcgaagcag cggctccgcg agctccaggc
     3721 gctgacgcat cgcggcctcc atggcggcgc ggtgcgattc gtggaagggg tggatgcttt
     3781 cggccattgg acgcctcccg gacgcggcag cctatcgcaa acgccctcgg cgttccagcg
     3841 cgatgcgccg gcccgcgaaa tgcaaaaagg cccgccgaag cgggcccctc tcgtcgcgcg
     3901 cggggcgcgg ccggcgccgg ccgatcagcg ctcgcgct
//
LOCUS       DASVLN010000473         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_346010, whole genome shotgun sequence.
ACCESSION   DASVLN010000473 DASVLN010000000
VERSION     DASVLN010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346010"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..>1512)
                     /locus_tag="VF453_11655"
     CDS             complement(150..>1512)
                     /locus_tag="VF453_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7688358.1"
                     /translation="APSGAARERPDAAGGRTSGPAFAAMVALAALQALLRNKTRSLLT
                     MLGVFIGVAALIAMIAVGQGANEAVRRQIESLGTNLFVVLPGATTSAGVRGGFGSAST
                     LTVADAQAVMREATQAQAVSYLIRQSGQVQYRDRNWTTLVQAVTASYPPTTHWRVAEG
                     RALTDDDDRAAALVAVIGETVRRQLFAADENPVGATVLVRGLPLRVVGLYAAKGQTAY
                     GQDQDDLVMIPFGTGERKILGVAAPSQAGAAASPYPGLPNPFGLQPRLTGYVNQIYVQ
                     AASAGQVQAAIDEVTAVLRRRHRIRATDDPDFNVRNLSQIADAAEGSSRVMAALLAVV
                     ASISLLVGGIGIMNILLVSVTERTREIGLRMAIGAQRGQVLLQFLAEAVFMSVVGGVA
                     GIVVGAVSTQTIAAVAKWPTIVSPAAVAGGFAFSAAVGVFFGYYPARKAARLDPIEAL
                     RYE"
BASE COUNT          212 a          570 c          545 g          185 t
ORIGIN      
        1 ccgcaagcgt cgcgccggat gacacgacga cgggatcgtc ctgcgtgaag ccgccggccg
       61 ccggcccggg cggcggcgcg tagtcgggcc cggccgcgca gccggcgagc accaggccca
      121 gcccgagcgc atgccaccgc cgccgcacgt cactcatagc gcagcgcctc gatgggatcc
      181 aggcgtgccg ccttgcgcgc cgggtagtag ccgaagaaga cgccgaccgc cgcggaaaac
      241 gcgaacccgc ccgcgacggc ggcgggcgag acgatggtcg gccatttcgc cacggcggcg
      301 atggtctgcg tggagaccgc gccgacgacg atgcccgcga cgccgcccac gacgctcatg
      361 aacaccgcct cggcgaggaa ctgcagcagc acctgcccgc gctgggcgcc gatcgccatg
      421 cgcaagccga tctcgcgcgt gcgctcggtc accgagacga gcaggatgtt catgatgcca
      481 atgccgccca ccagcagcga gatcgacgcc acgaccgcca ggagcgccgc catcacgcgg
      541 ctgctgccct cggccgcatc cgcgatctgg ctcaggttgc ggacgttgaa gtcgggatcg
      601 tccgtggcgc ggatgcggtg ccggcgcctc aggacggccg tcacctcgtc gatcgcggcc
      661 tgcacctggc ccgccgaggc cgcctggacg tagatctggt tcacgtagcc cgtcaaccgg
      721 ggctgcaggc cgaagggatt gggcaggccc gggtacggcg acgcggccgc ccccgcctgg
      781 ctcggcgcgg cgacgccgag gatctttcgc tcgccggtgc cgaaggggat catgacgagg
      841 tcgtcctggt cctgcccgta ggcggtctgt cccttggcgg catacagccc gaccacgcgc
      901 agcggcagcc cgcgcaccag cacggtcgcc ccgaccgggt tctcgtcggc ggcgaagagc
      961 tggcgccgca cggtctcgcc gatcacggcc accagcgcgg ccgcgcgatc gtcgtcgtcg
     1021 gtgagcgccc ggccttcggc gacgcgccag tgggtggtcg gcggatagct cgccgtcacg
     1081 gcctgcacga gcgtcgtcca gttgcggtcg cggtactgca cctgccccga ctggcggatc
     1141 aggtagctca ccgcctgcgc ctgcgtggcc tcgcgcatga cggcctgggc gtcggcgacg
     1201 gtcagcgtgg aggcgctgcc gaagccgccg cgcacgccgg cggaggtcgt ggcgccgggc
     1261 agcacgacga acaggttggt gcccaggctc tcgatctgcc ggcgcacggc ctcgttggcg
     1321 ccctgcccca cggcgatcat cgcgatgagc gccgccacgc cgatgaagac ccccagcatc
     1381 gtgagcagcg accgggtctt gtttcgcagc agggcctgca gggccgccag cgccaccatc
     1441 gccgcgaagg ccggcccgga cgtccgcccg ccggcggcgt cggggcgttc ccgcgccgca
     1501 cccgaaggcg cc
//
LOCUS       DASVLN010000474         4434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_185587, whole genome shotgun sequence.
ACCESSION   DASVLN010000474 DASVLN010000000
VERSION     DASVLN010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185587"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..776)
                     /locus_tag="VF453_11660"
     CDS             complement(129..776)
                     /locus_tag="VF453_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7688359.1"
                     /translation="MPAVFTPRGALVVRLAASAILALLVAGIALLVRHARAYEQLGEP
                     IAQPIPFSHKHHVSDDGIDCRFCHTTVETEANAGMPATSTCLGCHSQLFRDQPLLALL
                     HQSEASGRPVPWTRVHVLPDFVFFNHSVHVAKGVACVECHGRVDQMPIEWRARPLQMQ
                     WCLDCHRDPAPRLRPPSQVFRLDAPRPPLAEASLLARAAHLESRRRMTDCSTCHR"
     gene            complement(985..1509)
                     /locus_tag="VF453_11665"
     CDS             complement(985..1509)
                     /locus_tag="VF453_11665"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HEX7688360.1"
                     /translation="MKPKIALTLAAAALALGSASAGAATTDWGTLGPVADVAYVTYHT
                     PGAVDDIYTFNLTGQWSDVDAYAEEFESRSVGLQNATFTLYSGAVGSGTQVGQSFSFS
                     NTATENVYHSLAAGNYYVELTGMGVNQGSAYDFELFANESGPPSSVPEPGSTALLLSG
                     IGLFGFMAMRRRQR"
     gene            complement(1842..2855)
                     /locus_tag="VF453_11670"
     CDS             complement(1842..2855)
                     /locus_tag="VF453_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688361.1"
                     /translation="MHPSPRDHRVRIASYILAGLALVGVLALHLLVPLLAGMLVHELV
                     QSVAPRAQRFFRTKRAKVIVVALYAAVIVGAICGGVFALITFFRHDAQNLPKLLAKVS
                     EILDQVRAGIPPFLAAYMPDDIAALQQSAIEWLRGHVAELQMIGAHTLVTIAETIIAL
                     VIGAVLSLREVDPHTPRGPLALALTARIERFATAFRRVALSQLSISFVNTCFLAAYLL
                     IGLSAFGIRLPLTKTMIGATFVVGLLPIVGNLISNSIVVIVSLAHSPAVAVASLVFLV
                     VIHKLEYFLGARIVGSRIKSQIWELLLAMMAMEAMFGIPGLVAAPIFYAYLKSELMEA
                     GLI"
     gene            complement(2926..3660)
                     /locus_tag="VF453_11675"
     CDS             complement(2926..3660)
                     /locus_tag="VF453_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7688362.1"
                     /translation="MAPDDILLEVEGLHAGYGRAEVLSGLALRAARGSVVAVVGPNGA
                     GKSTLLNALMGVLPARGEIRFDGRPAQRLTLEERVAQGIALVPETRALFATMPVADNL
                     LLGGYRRRREPLAQRRARLDEIYALFPRLHERQAQAAGTLSGGERQMLAIGRALMGSP
                     RLLMLDEPSLGLAPLVVRDIFAIIERLRATGVTILLIEQNARAAMAVADHAYVLETGA
                     IALSGPAAQLAADPRVIDTYLGKARV"
     gene            complement(3660..>4434)
                     /locus_tag="VF453_11680"
     CDS             complement(3660..>4434)
                     /locus_tag="VF453_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7688363.1"
                     /translation="TPPAHGETVLSVQGARKQFGGLVAVNDVGFDVRAGEIVGLIGPN
                     GAGKSTLFNLITGVLPLTRGRIDFRGERVDGLPSRAIARRGMSRTFQHVRMIADMSVL
                     ENVALGAHKRGRAGVLRAMLRLDRADERRLMAEAWRQLDRIGMAALADEPAGNLALGQ
                     QRLMEIARALCTDPSLLLLDEPAAGLRLKEKHALARVLAQLRAEGMSLLLVEHDMDFV
                     MTLADRLVVVEFGTKLTEGPPDAVREDPAVRAAYLGVEH"
BASE COUNT          672 a         1489 c         1685 g          588 t
ORIGIN      
        1 ccgcgatgca cgtgcaccgg ttgcggcgcg gggtcgccgg tcgcatgggg ggtatcgggc
       61 gcgggggagg cggtcatgcg ggcatgggcg agggggcgcg tcgcgtcggc aagggtcgtg
      121 ccgcgccttc agcgatggca ggtggagcag tccgtcatgc ggcggcggct ctcgagatgc
      181 gccgcgcgcg cgagcaggct cgcctcggcc aatggcggcc gcggggcgtc gagcctgaac
      241 acctgcgacg gcggccgcag ccgcggcgcg gggtcgcggt ggcagtccag gcaccattgc
      301 atctgcagcg gtcgcgcgcg ccattcgatg ggcatctggt cgacgcgccc gtggcattcg
      361 acgcaggcca cgcccttggc gacgtgcacg ctgtggttga agaagacgaa gtccggcaga
      421 acgtgcacgc gcgtccacgg cacgggccgg ccgctggcct cgctctggtg cagcagcgcg
      481 agcagcggct ggtcgcggaa cagttgcgag tggcagccca ggcaggtgct ggtggccggc
      541 atgccggcgt tcgcctcggt ctcgaccgtc gtgtggcaga agcggcagtc gatgccgtcg
      601 tcggagacgt ggtgcttgtg gctgaacggg atgggctggg cgatgggctc gccgagttgc
      661 tcgtaggcgc gcgcgtggcg caccagcagc gcgatgccgg cgaccagcag cgccaggatc
      721 gcgctcgccg cgagccgcac gaccagcgcg ccgcgcggcg tgaagaccgc gggcatcagc
      781 ggcgacgtcg cggcgggcgc gacgggcggg cacaggagga ggcggcgggc gtcatcggcg
      841 cggcaggcag ggcgagcgtg cgcgcttcgg caatcaacgt gctcgcagcg acgggcgacg
      901 gtgatcgcga cgcggccacc ggcggccgcg gggaaaagaa aacgggcccc gcgggacccg
      961 tccatgtcac ggctcgaccg tggatcagcg ctggcggcgg cgcatcgcca tgaagccgaa
     1021 caggccgatg ccgctgagca gcagcgcggt gctgccaggt tccggcacgc tcgacggcgg
     1081 gccggactcg ttggcgaaca gctcgaagtc gtacgccgac ccctgattca cgcccatgcc
     1141 cgtgagttcc acgtaatagt tgccggcggc gagcgagtga tacacgttct ccgtggcagt
     1201 attgctgaaa gagaaggact ggccgacctg cgtgcccgag ccgacggcgc ccgagtacag
     1261 cgtgaaggtc gcgttctgca ggcccaccga gcgcgactcg aactcttccg cgtacgcgtc
     1321 gacgtcggac cactgcccgg tcaggttgaa ggtgtagatg tcgtccaccg caccaggggt
     1381 gtggtacgtg acgtaggcga cgtcggcaac ggggccgagc gtgccccagt cggtggtggc
     1441 cgcaccggcg ctggcggagc cgagtgcgag agctgctgca gccagggtca gcgcaatctt
     1501 gggtttcatg tcgtcatcct cgaagtcagg ggtttcggag gtgaccggca aaggaccgat
     1561 cgcggccatg tcggcaagcc acggtcccgc cctgctgcac tgcaacaaac gcttgcaatc
     1621 ctttcagaac gccggaccgg cgtcacgcga cggtcgcgcg ggcgcctcgg cggccagggc
     1681 gggtggccgg cctgcggtcg agcatcttga tacctaacgg ggtatgctga cgacgccggc
     1741 atgcggcata cccgaacccg ttccgggcaa gcgcgcaccg gttcggggcg aggcgctcga
     1801 acgcggatgc cgcaggcgcc gcccgtcgtc tcccgcggtg ctcagatcag cccggcctcc
     1861 atcagctcgc tcttcaggta ggcgtagaag atcggcgctg ccaccagccc ggggatgccg
     1921 aacatcgcct ccatcgccat catcgcgagc agcagctccc agatctgcga cttgatccgc
     1981 gagcccacga tgcgcgcgcc caggaagtac tccagcttgt ggatcacgac caggaagacg
     2041 agcgacgcga ccgccaccgc gggcgagtgc gcgaggctca cgatcaccac gatgctgttg
     2101 gagatcaggt tgccgacgat gggcagcagg ccgacgacga acgtggcgcc gatcatcgtc
     2161 ttggtcagcg gcaggcggat gccgaacgcg ctcaggccga tgagcaggta ggccgcgagg
     2221 aagcacgtgt tgacgaacga gatcgacagc tgcgacagcg cgacgcggcg gaaggcggtc
     2281 gcgaagcgct cgatgcgtgc ggtcagcgcg agcgcgagcg ggccgcgcgg ggtgtgcggg
     2341 tcgacctcgc gcagcgacag caccgcgccg atcacgagcg cgatgatggt ctcggcgatc
     2401 gtcaccagcg tgtgggcgcc gatcatctgc agctcggcga catggccgcg cagccactcg
     2461 atcgccgact gctgcagggc cgcgatgtcg tcgggcatgt aggccgcgag gaagggcggg
     2521 atgccggcgc gcacctggtc gaggatctcc gagaccttgg ccagcagctt gggcaggttc
     2581 tgggcgtcgt ggcggaagaa cgtgatcaac gcgaacacgc cgccgcagat cgcgccgacg
     2641 atgacggcgg catacagcgc gaccacgatc accttcgcgc gcttggtgcg gaagaagcgc
     2701 tgcgcgcgcg gggcgaccga ctgcaccagc tcgtgcacca gcatccccgc gagcagcggc
     2761 acgagcaggt gcagcgcgag cacgcccacc agcgcgaggc cggccaggat gtaggaggcg
     2821 atgcggacgc ggtggtcccg gggcgagggg tgcatggtgg cctggtggag tggcgttgcc
     2881 gcgttggagg ggcggtggac gtgaaagtgc cgccggccgg gccggtcaga cgcgcgcctt
     2941 gccgaggtag gtgtcgatca cgcgcgggtc ggcggcgagc tgggccgccg ggcccgacag
     3001 cgcgatcgcg ccggtctcga gcacgtaggc gtggtccgcc acggccatgg cggcgcgcgc
     3061 gttctgctcg atcagcagga tggtgacccc ggtcgcgcgg aggcgttcga tgatggcgaa
     3121 gatgtcgcgc acgaccagcg gcgcgaggcc gaggctcggc tcgtcgagca tcagcaaccg
     3181 cggcgagccc atcagcgcgc ggccgatggc gagcatctgg cgctcgccgc ccgacagcgt
     3241 gccggcggcc tgggcctggc gctcgtgcag gcgcgggaag agggcgtaga tctcgtccag
     3301 gcgcgcgcgg cgctgggcga gcggctccct gcgccggcga tagccaccga ggagcaggtt
     3361 gtcggcgacg ggcatcgtcg cgaacagggc gcgtgtctcg ggcacgagcg cgatgccctg
     3421 cgcgacgcgt tcctccagcg tcaggcgctg cgcgggccgg ccgtcgaagc ggatctcgcc
     3481 gcgcgccggc agcacgccca tcagcgcgtt cagcagcgtg ctcttgcccg cgccgttggg
     3541 cccgaccacc gcgaccacgc tgccccgcgc ggcgcgcagc gcgaggcccg agagcacttc
     3601 ggcgcggccg tagccggcat gcagcccctc gacctcgagc aggatgtcgt ccggggccat
     3661 cagtgctcca ccccgaggta ggcggcgcgc accgccggat cctcgcgcac ggcgtcgggc
     3721 gggccctcgg tcagcttggt gccgaactcg acgaccacca gccggtcggc gagcgtcatg
     3781 acgaagtcca tgtcgtgctc caccagcagc aggctcatgc cttcggcgcg cagctgcgcg
     3841 agcacccgcg ccagcgcgtg cttctccttc aggcgcagcc cggcggcggg ctcgtcgagc
     3901 agcagcagcg acggatcggt gcacaacgcg cgcgcgatct ccatcagccg ctgctggccg
     3961 agcgcgaggt ttccggccgg ctcgtccgcg agcgccgcca tgccgatgcg atcgagctgg
     4021 cgccaggctt cggccatcag gcggcgctcg tcggcgcgat cgaggcgcag catcgcgcgc
     4081 aggacgccgg cgcggccgcg cttgtgggcg ccgagggcga cgttctcgag cacgctcatg
     4141 tccgcgatca tgcgcacgtg ctggaaggtg cgcgacatgc cgcgcctggc gatcgcgcgc
     4201 gaaggcaggc cgtcgacgcg ctcgccgcgg aagtcgatgc gtccgcgcgt gagcggcagc
     4261 acgccggtga tcaggttgaa cagcgtcgat ttgcccgcgc cgttgggccc gatcaggccg
     4321 acgatctcgc ccgcgcgcac gtcgaaaccg acgtcgttga ccgccaccag gccgccgaac
     4381 tgcttgcgtg cgccctgcac ggacagcacc gtctcgccgt gcgccggcgg cgtc
//
LOCUS       DASVLN010000475         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_346116, whole genome shotgun sequence.
ACCESSION   DASVLN010000475 DASVLN010000000
VERSION     DASVLN010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346116"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1677)
                     /locus_tag="VF453_11685"
     CDS             complement(70..1677)
                     /locus_tag="VF453_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7688364.1"
                     /translation="MQPDAAAAPPEARDPRDPSDRRLATAHRWAATLVLSFASGLPLA
                     LSGTALQVWLTMDGVDLARIGFLTLLGLPYTFKFLWAPLMDRVDLPLLGRRRGWIVLT
                     QALLALALWALAGVSPLERLGTFAALGVAIAFLSASQDVVIDAYRTDLLPARERGVGS
                     SLYVTGYRLAMILSGGVALIWTDRASGYGMSWPEVYRVMAALMAGAAVVSAIALPRLP
                     QPPARLSDPGRTPIGRDLRGLVAVIAAVVAGVAITRAFLQPLAGWLLSPLFGAAVAAN
                     SLPARWIDLLALVLGLALTLPLAAAAARLARFQTLLDGLGAYFAQPRAWSFLGFIVFY
                     KLSDAFALSLMTPFLLKGMGYGPAEVGVVNKLLGLAFTIVGALLGGTLMLRLRLARAL
                     LAFGVLQMASNLVFWWLSVHGKDTLPGLLIPAFDLGFVRIADPMPVDGGLLLAVSSEA
                     VASGMGTTAFLAFLMGLTNRRFTATQFALLSAFASVGRVWVGPVAGVLSQAIGWPAFF
                     LVAMALALPALAWLVALWRPISALEAR"
     gene            complement(1712..>3504)
                     /locus_tag="VF453_11690"
     CDS             complement(1712..>3504)
                     /locus_tag="VF453_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7688365.1"
                     /translation="PEPASPVPAPAASAAAPASAPPKVLRYALPVAETGFDPAQVPDA
                     YSREIIDNIIEPPLRFDLLSRPVRLRTATAAALPEMSADFRELTLRIRPGIFFADDPA
                     FHGRPRELTAEDYAYTLRRHFDPRVHSVAYADLVEDDIVGLQALRERALARNAPFDYD
                     APVEGLRVMDRYTLRIRFGHPDPRFPLKLAETMFAGAVAREVAEYYGDAIMEHPVGTG
                     PYRLVQWRRSSFMAFERNPRFRAETYDEQPTPGDADAEATARALHGRREPMIDRVEVS
                     IIEEPQPTWLSFVRGDFDFTMVPYEFASQAAPGGQLAPNLARRGMKLHFVPQADVIYT
                     YFNMDDPVVGGYAPAQVALRRAIALAYDNDEEIRLLRKPLAIPAQGIVVPLVQGYDPA
                     YRSAMSDYDPARARALLDLYGYVDRDHDGWRERPDGSPLLLHVATQPDTTARALDELW
                     QKRMDAIGVHVVFDRRQWTEQMKRSRAGTLQMWMLGTSADEPDAEDLLSTAYGPNKGQ
                     GNFSRFDLPEYNDLFRRIQELPDGSERDALIARAQKLLLAYMPVKAHVHRVRMVLSQP
                     WLEGYPANPFVNGYWRFLDVDPARRAADGH"
BASE COUNT          527 a         1242 c         1324 g          411 t
ORIGIN      
        1 agcgtcgcca gcgcgcgcag cgccgcgcgg cgtccggggt cggcggcgcc ggtcatcgat
       61 cgctgcggtt cagcgcgcct cgagcgccga gatcggccgc cacagcgcga cgagccaggc
      121 gagcgccggc agcgccagcg ccatcgcgac gaggaagaag gcgggccagc cgatggcctg
      181 cgacaacacg cctgccaccg gcccgaccca gacccggccc accgacgcga acgccgacag
      241 cagcgcgaac tgcgtcgccg tgaagcgccg gttggtcagg cccatcagga acgcgaggaa
      301 ggccgtcgtg cccatgccgc tggccacggc ctcgctcgag accgccagca acaggccgcc
      361 gtcgaccggc atcgggtccg cgatgcggac gaagccgagg tcgaacgccg ggatcagcag
      421 tcccggcagc gtgtccttgc cgtgcactga cagccaccag aacacgaggt tgctcgccat
      481 ctgcagcacg ccgaacgcga gcagcgcccg cgccagccgc agccgcagca tgagcgtgcc
      541 acccagcagc gcgccgacga tcgtgaaggc gagcccgagc agcttgttga ccacgccgac
      601 ctcggccggc ccgtagccca tgcccttgag caggaagggc gtcatgagcg acagcgcgaa
      661 cgcgtcgctc agcttgtaga agacgatgaa gccgaggaag ctccacgcgc gcggctgcgc
      721 gaagtaggcg cccaggccgt cgagcagcgt ctggaagcgc gcgagccgcg ccgccgcggc
      781 cgccagcggc agcgtgagcg cgagccccag caccagcgcg agcaggtcga tccagcgcgc
      841 gggcagcgaa ttggccgcga ccgcggcgcc gaacagcggc gacagcagcc agccggcgag
      901 cggctgcagg aaggcgcggg tgatcgcgac gcccgcgacc accgccgcga tcaccgccac
      961 gaggccgcgc aggtcgcgcc cgatcggcgt gcggccggga tcggacagcc gcgccggcgg
     1021 ctgcggcagc cgcggcagcg cgatcgccga caccaccgcc gcgcccgcca tcagcgccgc
     1081 catcacgcgg tagacctcgg gccagctcat gccgtagccg ctcgcgcggt ccgtccagat
     1141 cagcgccacg ccgccggaca ggatcatcgc cagtcgatac cccgtcacgt acagcgacga
     1201 gccgaccccg cgctcgcgcg ccggcagcag gtcggtgcgg taggcgtcga tcacgacatc
     1261 ctgcgacgcg ctgaggaagg cgatcgcgac gcccagcgcc gcgaaggtgc cgaggcgctc
     1321 cagcggcgag acgccggcga gcgcccacag cgccagcgcg agcagcgcct gcgtgagcac
     1381 gatccagccg cgccgtcggc cgagcagggg caggtcgacg cgatccatca gcggcgccca
     1441 caggaacttg aacgtgtacg gcagcccgag cagcgtgagg aagccgatgc gcgcgaggtc
     1501 gacgccgtcc atcgtcagcc agacctgcag cgccgttccg ctcagcgcga gcggcaggcc
     1561 ggaggcgaag ctcaggacga gcgtcgccgc ccagcggtgg gcggtggcca ggcggcggtc
     1621 gctggggtcg cggggatcgc gcgcctcggg cggcgccgcg gcggcgtcag gttgcatcgc
     1681 ggcattctag gagccggccg cctccggtcg gtcagtgccc gtcggcagca cggcgggccg
     1741 ggtcgacgtc caggaagcgc cagtagccgt tgacgaacgg gttggccgga tagccttcca
     1801 gccacggctg cgacagcacc atgcgcacgc ggtgcacgtg ggccttgacc ggcatgtagg
     1861 cgagcagcag cttttgcgcg cgtgcgatca gcgcgtcgcg ctccgatccg tcgggcagct
     1921 cctggatgcg gcggaagagg tcgttgtact ccggcaggtc gaagcgcgag aagttgccct
     1981 gccccttgtt cggcccgtag gcggtgctca gcaggtcctc ggcgtcgggc tcgtcggccg
     2041 aggtgcccag catccacatc tgcagcgtgc cggcgcgcga gcgcttcatc tgctccgtcc
     2101 actggcggcg gtcgaagacg acgtgcacgc cgatcgcgtc catgcgcttc tgccacagct
     2161 cgtcgagcgc gcgcgcggtc gtgtcgggct gcgtggccac gtgcagcagc agcgggctgc
     2221 cgtcggggcg ctcgcgccag ccgtcgtggt cgcggtcgac gtagccgtag aggtcgagca
     2281 gcgcccgcgc gcgcgccggg tcgtagtcgc tcatcgcgct gcgataggcc gggtcgtagc
     2341 cctgcaccag cggcacgacg atgccctgcg cggggatcgc gagcggcttg cgcagcaggc
     2401 ggatctcctc gtcgttgtcg taggcgagcg cgatcgcccg ccgcagcgcg acctgcgccg
     2461 gcgcgtagcc gccgacgacg gggtcgtcca tgttgaagta ggtgtagatc acgtccgcct
     2521 gcggcacgaa gtgcagcttc atgccgcgcc gcgcgaggtt gggcgcgagc tgcccccccg
     2581 gcgcggcctg cgacgcgaac tcgtagggca ccatcgtgaa atcgaagtcg ccgcgcacga
     2641 aggacagcca ggtcggctgc ggctcctcga tgatcgagac ctcgacgcgg tcgatcatcg
     2701 gctcgcgccg gccgtgcagc gcacgcgcgg tggcctcggc gtcggcgtcg ccgggggtcg
     2761 gctgctcgtc gtaggtctcg gcgcggaagc gcgggttgcg ctcgaaggcc atgaacgacg
     2821 agcgccgcca ctgcacgagc cggtacggcc ccgtgcccac cgggtgttcc atgatcgcgt
     2881 cgccgtagta ctcggcgacc tcgcgcgcga ccgcgccggc gaacatcgtc tcggcgagct
     2941 tgagcgggaa gcgcgggtcg ggatggccga agcggatgcg cagcgtgtag cggtccatca
     3001 cgcgcaggcc ctcgaccggc gcgtcgtagt cgaacggcgc gttgcgtgcc agcgcgcgct
     3061 cgcgcagcgc ctgcaggccg acgatgtcgt cctcgaccag gtcggcgtag gcgacgctgt
     3121 gcacgcgggg gtcgaagtgg cgccgcagcg tgtaggcgta gtcctccgcg gtgagctcgc
     3181 gcgggcggcc gtggaaggcc gggtcgtcgg cgaagaagat gcccgggcgg atgcgcagcg
     3241 tgagctcgcg gaagtcggcg gacatttccg gcagcgccgc ggccgtggcc gtgcgcaggc
     3301 gcaccgggcg cgagagcagg tcgaagcgca gcggcggctc gatgatgttg tcgatgatct
     3361 cgcgcgagta ggcgtccggc acctgcgcgg gatcgaagcc ggtctcggcc accggcaggg
     3421 cgtagcgcag caccttgggc ggcgcggacg cgggggcagc ggccgaggcc gcgggcgccg
     3481 gcacgggcga cgccggctcg ggag
//
LOCUS       DASVLN010000476         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_346149, whole genome shotgun sequence.
ACCESSION   DASVLN010000476 DASVLN010000000
VERSION     DASVLN010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346149"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VF453_11695"
     CDS             complement(<1..196)
                     /locus_tag="VF453_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX7688366.1"
                     /translation="MTLTNPRRCLHVAATAAACALPLAALAHPGSLAEHAAHPHGFAD
                     GFVHPFTGLDHLGAMLAVGLW"
     gene            complement(254..556)
                     /locus_tag="VF453_11700"
     CDS             complement(254..556)
                     /locus_tag="VF453_11700"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX7688367.1"
                     /translation="MNLTPREKDKLLLFTAALLAERRRARGLKLNHPEAVALISAAIL
                     EGARDGRSVADLMSEGRRVLTRDDVMPGIAEMIPEIQVEATFPDGTKLVTVHQPIP"
     gene            630..>2320
                     /locus_tag="VF453_11705"
     CDS             630..>2320
                     /locus_tag="VF453_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX7688368.1"
                     /translation="MTSAATAAHDPAPAVRTPAPQRVVKVRRDYNSWVAQETLEDYAL
                     RYTPQSFRRWSELRVANTAFGAASFLVLEAVGATLMVQDGFVNAFWAILATGLVIALA
                     GWPIALHAARHGLDMDLLTRGAGFGYLGSTVTSLVYASFTFIFFALEAAVMAYALELA
                     FDIPPAWGYLACALAVLPLVAHGVTAIGRLQVWTQPLWLALLVLPYVFVFHANPHALA
                     GLWRYAGEDGRGGAFDLLKFGAATTVGIALMTQMGEQADYLRFMPARTAANRRRWLAC
                     VAVGGPGWVLPGVLKMLGGALLAWIAIGHSVPADRAVDPNQMYLAAWDTVFGRPALAV
                     AATTVFVVLSQLKINVTNAYAGSLAWSNFFARLTHSHPGRVVWVAFNTAIALMLMELD
                     VFTMLGQVLGLYANIAISWLMAVVADLVVNKPLGLSPRGIEFRRAYLYDVNPVGTGAM
                     GIASALGIAAYLGVFGPAAQAFSALISLVAAFVASPLIAWATRGRYYLARDDEDAAEA
                     AGARPRLLRCCICERDYEGADTARCPAYQGPICSLCCSLDARCGDLCKPHARLAT"
BASE COUNT          273 a          828 c          844 g          375 t
ORIGIN      
        1 tccagaggcc cacggccagc atcgcgccca ggtggtcgag gccggtgaac ggatgcacga
       61 agccgtccgc gaagccgtgc ggatgcgcgg cgtgctccgc gagcgagccc gggtgggcga
      121 gggcggcgag cggcagcgcg cacgccgcgg cggtcgcggc gacatgcagg cagcggcggg
      181 gattcgtcag ggtcatgtcg tcctcgtgtc gtgtgggaag cgcgtgcgtc gacctggtgc
      241 ggtcatgcac gattcaggga atcggttggt gcacggtcac cagcttggtg ccgtcgggga
      301 aggtggcctc gacctggatt tccgggatca tctcggcgat gccgggcatc acgtcgtccc
      361 gcgtgaggac gcgccggccc tcgctcatga ggtcggcgac gctgcggccg tcgcgcgcgc
      421 cctcgaggat ggcggcgctg atcagcgcga ccgcctcggg gtggttgagc ttcaggccgc
      481 gcgcccggcg gcgctcggcc agcagggcgg cggtgaacag caggagcttg tccttttcgc
      541 gcggcgtgag attcatcgca cgagcctatt gcaggtttgg tgccacgcgc aagcccgggg
      601 cggcacgggt cttgaatgcg actgccgcga tgacctccgc cgccaccgcc gcccacgatc
      661 cggcgcccgc cgtccgcacg cccgcgccgc agcgcgtggt caaggtgcgc cgcgactaca
      721 acagctgggt cgcgcaggag acgctggagg actacgcgct gcgctatacg ccccaatcct
      781 ttcgccggtg gtcggaactg cgcgtcgcca acaccgcctt cggcgcggcg tccttcctcg
      841 tgctggaggc ggtgggcgcg acgctgatgg tgcaggatgg cttcgtcaac gcgttctggg
      901 cgatcctcgc gaccggcctc gtgatcgcgc tcgcgggctg gccgatcgcg ctgcacgccg
      961 cgcgccacgg cctggacatg gacctgctca cgcgcggcgc cggcttcggc tacctgggct
     1021 cgaccgtcac ctcgctcgtg tacgcgagct tcacgttcat cttcttcgcg ctcgaggcgg
     1081 ccgtgatggc ctacgcgctc gagctcgcct tcgacatccc gccggcctgg ggctacctcg
     1141 cctgcgcgct ggcggtgctg ccgctggtcg cgcacggcgt caccgcgatc gggcggctgc
     1201 aggtctggac gcagccgctg tggctcgcgc tgctggtgct gccctacgtg ttcgtgttcc
     1261 acgccaaccc gcacgcgctc gcgggcctgt ggcgctacgc cggcgaggac ggccgcggcg
     1321 gcgcgttcga cctgctcaag ttcggggccg cgacgacggt gggcatcgcg ctcatgaccc
     1381 agatgggcga gcaggccgac tacctgcgct tcatgcccgc gcgcacggcg gccaaccggc
     1441 ggcgctggct cgcgtgcgtg gcggtcggcg ggccgggctg ggtgctgccc ggcgtcctga
     1501 agatgctcgg tggcgcgctg ctggcgtgga tcgcgatcgg ccactcggtg cccgccgacc
     1561 gcgcggtcga cccgaaccag atgtacctcg cggcctggga cacggtcttc ggccggcccg
     1621 cgctggcggt ggccgcgacg accgtcttcg tcgtcctgtc gcagctcaag atcaacgtca
     1681 ccaacgcgta cgccggctcg ctcgcgtggt cgaacttctt cgcacgcctc acgcacagcc
     1741 atcccggccg ggtcgtgtgg gtggcgttca acaccgccat cgcgctgatg ctgatggagc
     1801 tcgacgtctt cacgatgctc ggccaggtgc tcggcctgta cgccaacatc gcgatctcgt
     1861 ggctgatggc ggtggtggcc gacctcgtcg tcaacaagcc gctcgggctg tcgccgcgcg
     1921 gcatcgagtt ccggcgcgcc tacctctacg acgtcaatcc ggtgggcacg ggcgcgatgg
     1981 gcatcgcctc ggcgctgggc atcgcggcct acctgggcgt gttcggcccg gcggcgcagg
     2041 cgttctcggc gctgatctcg ctcgtcgcgg ccttcgtcgc gtcgccgctg atcgcatggg
     2101 cgacgcgcgg gcgctactac ctcgcccgcg acgacgagga tgcggccgag gccgccggcg
     2161 cgcgaccgcg cctgctgcgc tgctgcatct gcgagcgcga ctacgagggc gccgacaccg
     2221 cccgctgccc ggcctaccag gggccgatct gctcgctgtg ctgctcgctc gatgcgcgct
     2281 gcggcgacct ctgcaagccc cacgcgcggc tggcgacgca
//
LOCUS       DASVLN010000477         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_78776, whole genome shotgun sequence.
ACCESSION   DASVLN010000477 DASVLN010000000
VERSION     DASVLN010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78776"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VF453_11710"
     CDS             complement(<1..450)
                     /locus_tag="VF453_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7688369.1"
                     /translation="MSSAATASHPASNALMASSVLVVDDEPDLLTLYELTLLREGLEV
                     ETAASVAQAREMLQARVFRAVISDMRLPDGSGLDLLRWLEESGRPEKVIVVTAYGSPE
                     NAVTALKAGAFDYLTKPVDLRQFRAVVASAMGRTRAPVPAPAPDAAPA"
     gene            complement(500..>2183)
                     /locus_tag="VF453_11715"
     CDS             complement(500..>2183)
                     /locus_tag="VF453_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7688370.1"
                     /translation="VLRPLAGSALRRIFGTYVAARSALGVALLAALTLIGNASGHYLL
                     APLALCGAYVMQALLWWATDQMRGLRALQSPRWQWWMTIGVDLLVFGAIHWIDPGSPL
                     NFAALLVLPVLMAGILTPRLPALATAAGVALMLLAAAGRLMGSVPDPAGLMSQAGLSG
                     IGLFVVALLAGELSMRLAHEERTARDSRERARQQAQLSRLVIEDMNDGVLVIDRQLRV
                     RAANPSARRLLVAQAARCPSPPFSLKLHPGWMILADEVDQAFARGSWPATPMDVNVQL
                     EDHTRRALHVRARFTRSRAEGHEDGASPAEEPLCVLFIEELRAVQARLRQERLAAMGR
                     ISAGVAHEIRNPLAAIAQAHALLEEDELRPDQRRLVNIMNDNIGRLRRIVDDVMEAAP
                     GALSPSRVLDATAEVASICSEWARTVNLALGSASRLQVLLPGQQVGVAFDPDHLRRVL
                     VNLLDNAIRHASAAPGAVRVTLKAVDDGWVVLAVASDGDPIAPDVEPYLFEPFHSTRS
                     RGTGLGLYICRELCERHGARIDFRRRDERHCNVFEVMMPRESLASAEGRLVL"
BASE COUNT          306 a          803 c          809 g          265 t
ORIGIN      
        1 cgcaggcgcc gcgtcgggcg cgggcgccgg caccggcgcg cgcgtgcgcc ccatcgccga
       61 ggcgacgacc gcgcggaact ggcgcaggtc gaccggcttg gtgagatagt cgaacgcccc
      121 cgccttcagc gccgtgacgg cgttctcggg cgagccgtag gcggtcacga cgatcacctt
      181 ctccggccgg ccgctctcct cgagccagcg cagcaggtcc aggccgctgc cgtccggcag
      241 ccgcatgtcg ctgatcacag cccgaaagac gcgcgcctgc agcatctcgc gcgcctgggc
      301 gaccgaggcc gcggtctcca cctcgaggcc ctcgcgcagc agcgtcagct cgtagagcgt
      361 gagcaggtcc ggctcgtcgt cgacgaccag cacgctgctg gccatgagcg cgttcgaggc
      421 gggatgggag gcggtggcgg cggagctcat cggaaaacct tcaggtgcgg gcggggcgcg
      481 gggcgttcac gaaggacgtt cacagcacca gccggccctc ggccgacgcc agcgactcgc
      541 gcggcatcat cacctcgaac acgttgcaat ggcgctcgtc gcggcggcgg aagtcgatcc
      601 gcgcgccgtg gcgctcgcac agttcacggc aaatgtacaa ccccaggccg gtgccgcggc
      661 tgcgcgtcga gtggaagggc tcgaacaggt agggctcgac gtcgggggcg atcgggtcgc
      721 cgtcgctcgc cacggccagc acgacccatc cgtcgtcgac cgccttgagc gtcacccgca
      781 ccgcgcccgg cgcggcgctc gcgtggcgga tggcgttgtc cagcaggttc acgagcacgc
      841 gccgcaggtg gtcgggatcg aaggcgacgc ccacctgctg gccgggcagc agcacctgca
      901 gccggctcgc gctgcccagc gcgaggttga cggtgcgcgc ccactcgctg cagatgctcg
      961 cgacctcggc ggtcgcgtcg agcacgcgcg agggcgacag cgcgccgggt gcggcctcca
     1021 tcacgtcgtc gacgatgcgg cgcaggcggc cgatgttgtc gttcatgatg ttgacgaggc
     1081 ggcgctggtc ggggcgcagc tcgtcctcct ccagcagcgc gtgcgcctgc gcgatggcgg
     1141 cgagcgggtt gcggatctcg tgcgcgacgc cggccgagat gcggcccatc gcggcgagcc
     1201 gctcctggcg cagccgcgcc tgcacggcgc gcagctcctc gatgaacagc acgcacagcg
     1261 gctcctcggc ggggctcgcg ccgtcctcgt ggccctcggc acggctgcgc gtgaagcggg
     1321 cgcgcacgtg cagcgcgcgg cgcgtatggt cctcgagctg cacgttgacg tccatcggcg
     1381 tcgcgggcca gctgccgcgc gcgaaggcct ggtcgacctc gtcggcgagg atcatccagc
     1441 ccggatgcag cttcagcgag aacggcggcg acggacagcg cgccgcctgc gcgacgagca
     1501 gccggcgcgc cgacgggttg gcggcgcgca cgcgcagctg gcggtcgatc acgagcacgc
     1561 cgtcgttcat gtcctcgatc acgagccggc tcagctgcgc ctgctggcgc gcgcgctcgc
     1621 ggctgtcgcg ggcggtgcgt tcctcgtggg cgaggcgcat cgacagctcg ccggccagca
     1681 gcgcgacgac gaacaggccg atgccgctca ggccggcctg cgacatcagc ccggccgggt
     1741 ccggcaccga gcccatgagc cgtcccgccg cggccagcag catcagcgcc acgccggcgg
     1801 ccgtcgccag cgcgggcagc cgcggcgtga ggatgcctgc catcagcacc ggcagcacca
     1861 gcagcgcggc gaagttcagc ggcgagccgg ggtcgatcca gtggatggcg ccgaagacga
     1921 gcaggtcgac gccgatggtc atccaccact gccagcgcgg cgactgcagc gcccgcaggc
     1981 cgcgcatctg gtcggtcgcc caccacagca gcgcctgcat cacgtaggcg ccgcacagcg
     2041 ccagcggcgc gagcaggtag tggcccgacg cgttgccgat cagcgtgagc gcggccagca
     2101 gcgcgacgcc gagcgccgat cgcgccgcca cgtaggtgcc gaagatgcgc cgcagcgcgc
     2161 tgccggccag cggcctcagc acc
//
LOCUS       DASVLN010000478        12214 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_132618, whole genome shotgun sequence.
ACCESSION   DASVLN010000478 DASVLN010000000
VERSION     DASVLN010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12214
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132618"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..1092)
                     /locus_tag="VF453_11720"
     CDS             complement(142..1092)
                     /locus_tag="VF453_11720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7688371.1"
                     /translation="MSAVDETVVAAAPRVLVLMAAYGGQRWIAEQLGSILAQRGVAVR
                     VRVSDDRSHDGTADVVRELAGRDDRVSLVVRAESSGSAGANFRSLLADADLSAADFVA
                     LSDQDDVWDSDHLVRGCEALRARRGAGGYSCAVRTFGAGRASTLSQCGRARSLDHLFE
                     GAGQGCSFVLTAELARRVQAACRDLPAAVAALHYHDWLIYVIARGASLDWIFDPRPGV
                     SYRQHGANEIGARSGAGAIRRRLRMIRSGWYKSQVRAASTIVRALGSLTPAHARFDEL
                     FLAPPSLLRRLRLAARLAFDGRRRLVDRGVLVWAALAGWL"
     gene            complement(1089..2114)
                     /locus_tag="VF453_11725"
     CDS             complement(1089..2114)
                     /locus_tag="VF453_11725"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7688372.1"
                     /translation="MTPESTLDVLVDARWPAGTGIGRMAGLYRTAAPVGMVLRDLDIR
                     CRIGSPRSPWAVDRALAQRPPSVARAVFWNPGFVPPASRRVPSVVTVHDLTHRHHYTV
                     AHRLYYDTVLRPLYRRCDLVVCISRHTRDEFLEWSGMPEARVAIVPNAIAPDFAAHSA
                     PLPEHKPFLFYAGNRRAFKNVPTMVRAFVASRLPQEGFELALTGAPDEALLAIARASG
                     VESSLRFLGFLTDAEVAAHYRAATCVPYLSRYEGFGLPILESWATDAPVLLANASCLP
                     EVGGDAALYVEPDDIEQIAQGMRDLCLDGTLRQTLIERGRERLRLFDPSVSAAKLWRA
                     VADVAAT"
     gene            complement(2111..3238)
                     /locus_tag="VF453_11730"
     CDS             complement(2111..3238)
                     /locus_tag="VF453_11730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7688373.1"
                     /translation="MKLLVYSSASAFGGHELIALKGLDALLADGHELDLVCSRINSRF
                     VDALSPLTTRHGDRLRLHLRDDYMRSLQIVRTWTNVPRMLSLWRLVRALQPDRVLALQ
                     GDIEQGSEIFLPARLAGVPVVSYVPMVMSGGDRGIRLARLRDALSRPIYALAARFVVI
                     APYFRDQALARGAPEAIVVSNCVDDDFLQAPVRRARVRMELGVREDERLAGYVGRIAY
                     DQKGLDRLVELLRGHREHFKSNRLLIVGSGPDAARLQADLRAQGVEDCVLRRDWMDDR
                     VGLFDAMDVFLCASRFEGVPLSVLEALSREVPVVAPPLPALAGKLPLDFCDVPFSVDD
                     FAARVRSHPVRRIADGVRPQPSTSGLARAEFAASFVRAVIS"
     gene            complement(3235..3843)
                     /locus_tag="VF453_11735"
     CDS             complement(3235..3843)
                     /locus_tag="VF453_11735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7688374.1"
                     /translation="MPATLLHFCVLAATGPAIAVRDMWMRARWRARGVRLARNVVLRS
                     HRPQGIELAADVSIGWGSLLLATTEMSALPPDASRLRIGAGTAVNEYCNLRASGGEIR
                     IGRKCLIAQMVTMVASNHGMDPAVPMIDQPWSVAPHSVIVGDDVWIGAGATLLPGARV
                     GNGAVIAAGAVVRGLVPAGEIWGGVPARFLRRRLPAESQGCA"
     gene            complement(3848..4621)
                     /locus_tag="VF453_11740"
     CDS             complement(3848..4621)
                     /locus_tag="VF453_11740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7688375.1"
                     /translation="MTVQVLIPAYNAGAFIAQTLESALEQSVEGMEIIVLDNRSTDDT
                     VRVARGYADRGVRVLAYDTNVGSLRNHNRALDLASADYVKLLSADDVLLPGMLAKQIA
                     ALEAHPDVGVVSCNCRVTDAMLNPRYDTSYLPGHLPGDVAILRCARRMANLVGAPSNT
                     LLRRSAVRDARFDTSLRWLADLDFVCQVLRRSNYLNIDEVGFLYRRHDTTDTVLSCPT
                     PVRVHDEFEFARRYGGGLAGRVRSVYRAALADVCAGTGA"
     gene            complement(4618..5988)
                     /locus_tag="VF453_11745"
     CDS             complement(4618..5988)
                     /locus_tag="VF453_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688376.1"
                     /translation="MTSSASPPHSVVARWREALGLDFHVVLTLLFRGWSVLAGASLLV
                     LLPLWLTPAQLGYHYTFASILALQIFFELGMNQVVSQITSHEIAHLKLAADGGLSGQP
                     ERIDRLTTLARMLRRWYAIAAGSFAVIVGAAGVAFFSRRGSSLDVQWLGPWLLMCSAT
                     ACNLYLSPSLAMIEGAGRVGEVARLRLMQSVLGFALTAAVLAAHCALWASTMVPVAAA
                     AGSALWLRRGGRMIEWLRARSLASSARVDWRREILPFQWRIALSWVSGYFIFQLFVPM
                     LFQNQGPVEAGRFGMVLAVFNAIQSIGMSWVYSCSPRMAAHISRAEGEDLHRTFLRAL
                     KPSTAFTALASATVVLGVWELSRAGFTFVHRISSTEVVCWMALVTVVNSVIFAFAVYM
                     RAHKEEPMMLASVVLAAVTLAIAHWASRVSVELTAQLYFCATALVSLPWASVLFRRYW
                     KRFAAP"
     gene            complement(6011..6898)
                     /locus_tag="VF453_11750"
     CDS             complement(6011..6898)
                     /locus_tag="VF453_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7688377.1"
                     /translation="MRNAFIEELVALAAVHPQVALVVGDLGYSVIEPFADRFPDRFIN
                     AGVAEQNMTGLAAGMASEGYHVFTYSIANFPTFRCAEQIRNDVDYHRLAVTVVAVGGG
                     LAYGALGYSHHAVQDYALMRSMPNMTIAAPGDPLETRACMRWLVENPGPSYLRLGKAG
                     EPRFHAEVPAVEPGRWLQVEAGDEDGGETLLATGAALGIALARSRKAAGPRPAVYSLP
                     LWGLEAKGAQAAQVARCASVTTFEDHLRDAGFGSWLMEAVGDSPELLPRLRAEALDPR
                     VCGTVGSQAMLNAHGGLTA"
     gene            complement(6908..7723)
                     /locus_tag="VF453_11755"
     CDS             complement(6908..7723)
                     /locus_tag="VF453_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7688378.1"
                     /translation="MTESIEDTRRLAGTIRRHALRMVHRARASHIGSALSIADIVAVL
                     YGRVMRIDPADPRAPDRDRFILSKGHACVAVYAALAARGFFAEEELLRYGQDHSELMN
                     HVSHKVPGVEFSTGSLGHGLPFGVGKALAAKRQGGDWRTFVVLSDGEWGEGSNWEACM
                     FASHHRLDNLVAIVDYNKLQSLTTVAETLALEPLADKFSAFGWTVREVDGHDHEALAE
                     ALGTAPWTLGKPSVLIAHTTKGKGVSYMENAVKWHYSSPSDEQLALALSELGD"
     gene            complement(7720..8793)
                     /locus_tag="VF453_11760"
     CDS             complement(7720..8793)
                     /locus_tag="VF453_11760"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7688379.1"
                     /translation="MDDTMRDIVEGDLRAVAARDLPWQMLSGCRVAVTGAGGFLGGYL
                     ARALLALHRLQRVDEPVRVLALVRDADRARARFADLAADIAAGRLEFLEWDLNTIAVP
                     ELGDVNYVLHAASQASPRFYGTDPVGTMLPNTIGTTALLAALRRAPDPRGLMFVSSSE
                     VYGAVAGGGTLEETGYGVVDPATVRACYAEGKRAGEAVCVAWHHQYGLPTWIVRPFHT
                     YGPGLTAEDGRVFADFAYNVARGENIVMNSDGTARRAFCYASDAVAGFLAVLLRGRPG
                     TAYNVANADAELSVMELAELLVGLYPEKRLRVDRRVDTDSPNYIPSPYNRLVPDTGRL
                     EALGWRAEVTPVEGFRRMIETLR"
     gene            complement(8803..10152)
                     /gene="rfbH"
                     /locus_tag="VF453_11765"
     CDS             complement(8803..10152)
                     /gene="rfbH"
                     /locus_tag="VF453_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HEX7688380.1"
                     /translation="MTETPVQFHKKTPDQLRAEIAALVQQYSDIVHAPRPFVPGESPV
                     PVSGKVIGAGELKMMVDASLDGWLTTGRFNAMFEERLAKFLGVKHLITVNSGSSANLV
                     AFSTLTSPRLGERAIRPGDEVIGVAAGFPTTVNPILQFGAVPVFVDVDLATHNIDASL
                     IEAAISPKTKAIMLAHSLGNPFNLEVVTAICRKHGLWLVEDCCDALGATYDGKLVGTF
                     GDIGTLSFYPAHHITMGEGGAVFTNDPELKLIAESFRDWGRDCYCPPGKDNTCNKRFC
                     WKLGDLPEGYDHKYTYSHLGYNLKISDMQAACALAQMDRVEDFIAARKANFAYLKERL
                     QQGGVAEFLHLPEATPKSDPSWFGFPVILKETAGAKRADLINFLDQNKIATRLLFAGN
                     LTRQPYMIGRNFRVSGELRNTDIVMNQTFWLGVWPGLSREQLDYVVDKLEEFFGLNF"
     gene            complement(10149..11225)
                     /gene="rfbG"
                     /locus_tag="VF453_11770"
     CDS             complement(10149..11225)
                     /gene="rfbG"
                     /locus_tag="VF453_11770"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEX7688381.1"
                     /translation="MTVDVDFWRGKRVFLTGHTGFKGSWLALWLQQLGAQVQGFALAP
                     PSEPALFDAARVAEGMASTLGDIRDLDAVRRAVEAFRPEIAIHMAAQPLVRLSYAEPV
                     ETYATNVMGTVHVLEALRRAPGVRAVVCVTTDKCYENREWAWGYREDEPMGGHDPYSN
                     SKGCAELVTSAYRRSFLQQAGVALASARAGNVIGGGDWATDRLVPDILRAFERGEPVV
                     IRNPSSTRPWQHVLEPLSGYLTLAQRLWQKGASVAEGWNFGPHDEDARPVRWIVERLA
                     TAWGEGAGWRLDAAAHPHEAHFLKLDISKARARLGWQPRWRLDEALARIVEWHRAWLA
                     GDDVRALCLRQIQQYSEALTSPAP"
     gene            complement(11233..12006)
                     /gene="rfbF"
                     /locus_tag="VF453_11775"
     CDS             complement(11233..12006)
                     /gene="rfbF"
                     /locus_tag="VF453_11775"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEX7688382.1"
                     /translation="MKAVILAGGLGTRLSEETAIRPKPMVEIGGKPILWHIMKMYSHH
                     GINDFVICCGYKGYLIKEYFANYFLHMSDVTFDMAHNKMEVHSKRAEPWCVTLVDTGD
                     TSMTGGRLRRVAKYVEDEEAFCFTYGDGVSALDISASIAFHRAHGKAATLTATYPPGR
                     FGALDIREGRVHSFQEKPKGDGAMINGGFFVLSPKVLEELHGDDTVWEQQPLMNLADR
                     GELMAFQYEGFWQPMDTLRDKVYLEELWASGKAPWKTWE"
BASE COUNT         2065 a         4161 c         4179 g         1809 t
ORIGIN      
        1 ggcggcggag gtacgtcaat cgggcatgct tatgggtgtt catccggttc cggctttgaa
       61 gttgacgggg ggtttggcga ttcccagtct ctcaaaacct ctccgggtga acacccgcaa
      121 caacctattg aaccttcaca gttagagcca tcccgcaagc gccgcccaga caaggacgcc
      181 ccggtcaacc aaccggcgac ggccgtcgaa agcaagtcgt gcggccagtc gcaggcgcct
      241 cagcagcgat ggcggcgcca ggaacaattc gtcgaagcgc gcatgtgccg gcgtgagtga
      301 accgagggcc cggacgatgg tcgatgccgc acgcacttga gacttgtacc aaccggaccg
      361 gatcatgcgg agtcggcgtc ggatcgcccc tgcgcccgag cgagcaccga tctcgttggc
      421 gccgtgctgc cgatagctga ccccaggccg cgggtcgaat atccagtcga gcgaagcgcc
      481 acgagcgatc acgtagatga gccagtcgtg gtaatgaagc gcggcgaccg ccgcgggcaa
      541 gtcccggcag gcggcctgca cgcggcgcgc cagttcggcg gtcaacacga acgaacagcc
      601 ttgcccggcc ccttcgaaca ggtgatcgag cgaacgcgca cggccgcatt gcgacagcgt
      661 cgacgcccgc cctgcgccga aggtgcgtac ggcgcaggaa tacccgccgg cgccgcgtcg
      721 cgcccgcagc gcctcgcacc cgcgcacgag gtggtccgaa tcccagacat cgtcctggtc
      781 cgacaatgcc acgaagtctg cagcggacag gtccgcatcg gcgaggagcg accggaaatt
      841 cgccccggca cttcccgagg attcggcacg cacgaccagc gacacgcgat cgtccctgcc
      901 cgcgagctcc cgcacgacgt ccgccgtgcc atcgtgtgag cgatcgtcag agacgcgcac
      961 gcgcaccgcc actccccgct gggcgaggat ggatccaagc tgctcggcga tccagcgttg
     1021 accgccataa gcggccatca gcacgagcac acgcggcgct gccgcgacga cggtctcgtc
     1081 gacggcactc atgtggcggc gacgtccgcg accgcgcgcc acagcttggc cgccgatacg
     1141 ctgggatcga acagacgcag acgctcgcgt ccgcgctcga taagcgtctg tcgcagcgtg
     1201 ccgtccaggc aaaggtcgcg catgccctgc gcgatctgct caatgtcatc gggctcgacg
     1261 tacaacgccg catcgcctcc gacttcaggc aggcacgatg cgttcgccag cagcacaggg
     1321 gcgtccgtgg cccaggactc aaggatcggc aatccgaacc cttcgtagcg cgacagatag
     1381 ggcacacatg tcgctgcgcg gtagtgcgct gcgacttcgg catcggtgag gaaaccaagg
     1441 aagcgcagcg aagactcgac gccggacgcc cgagcgatgg cgagcaaggc ctcgtcggga
     1501 gctcccgtca gcgctagctc gaagccttcc tgcggcagcc gcgaggcgac gaacgcacgc
     1561 accatcgtcg gcacgttctt gaacgcgcgc cgattgcccg cgtagaacag aaacggcttg
     1621 tgttcgggca gcggcgcgga gtgcgcggcg aaatcgggcg cgatggcgtt gggcacgatg
     1681 gcgactcggg cctcgggcat gccagaccac tcgaggaatt cgtcgcgcgt gtgcctggag
     1741 atgcagacaa ccaggtcgca tcgacggtac aaggggcgca acacggtgtc gtagtacagg
     1801 cgatgggcca cagtgtagtg atgccggtgc gtgaggtcgt gcaccgtgac gacagacgga
     1861 accctgcggc tggcaggtgg cacgaatcct gggttccaga acacggcccg ggcaacggat
     1921 ggaggccgtt gcgcaagcgc gcgatctacg gcccacggag acctgggact gcctatgcgg
     1981 cagcggatgt ccagatcgcg cagcaccatg ccgacaggcg ccgcggtgcg atagaggccg
     2041 gccatgcggc cgatgccggt gcccgctggc caccgggcat cgacgaggac gtcgagcgtc
     2101 gattcaggcg tcacgagatc accgcccgca cgaaggacgc cgcgaactcc gcccgcgcga
     2161 gcccggacgt cgatggctgg ggtcgcaccc cgtcggcgat gcgacgcacg ggatgcgacc
     2221 gcacgcgggc ggcgaagtcg tccaccgaga aaggtacgtc gcagaagtcc agcggcagct
     2281 tgcccgcgag tgcgggcagc ggcggtgcaa ctacgggcac ctcccggctc agagcctcca
     2341 acacggacag cggtaccccc tcgaagcgcg aggcgcatag gaacacgtcc atcgcatcga
     2401 acaggccgac gcggtcgtcc atccaatccc tccgaagcac gcagtcctcc acgccttgcg
     2461 cgcgcaggtc cgcctgcagg cgcgcagcgt cggggccgct gcccacgatc agcaggcggt
     2521 tggacttgaa atgctctcgg tgcccgcgaa gcagttccac caggcgatcg aggcccttct
     2581 ggtcgtaggc gatcctgccg acgtagccgg caaggcgctc gtcctcgcgg actccaagct
     2641 ccatgcgcac gcgggcgcgt cgcacgggcg cctggaggaa gtcgtcgtcg acgcaattgg
     2701 agacgacgat ggcttccgga gcaccgcgcg cgagcgcctg gtcgcgaaaa tacggcgcga
     2761 tcacgacgaa gcgcgcagcg agcgcataga tcggccgcga gagcgcgtcc cgcaggcggg
     2821 cgagacggat gccgcgatcc ccgcccgaca tcaccatggg gacgtagctg accaccggca
     2881 cgccggcaag cctggcaggc aggaagatct cactgccctg ttcgatgtcg ccctgcagcg
     2941 cgaggacgcg atcgggttgc agcgcccgca ccaggcgcca cagcgacagc atccgcggca
     3001 cgttcgtcca cgtgcgcacg atctgcagcg aacgcatgta gtcgtcgcgc aggtgcaggc
     3061 gcagccgatc gccgtgacgc gtggtcagcg gtgaaagtgc gtcgacgaac ctggaattga
     3121 tgcgcgagca gaccaggtcg agctcgtggc cgtcggccag cagcgcgtcc agccccttga
     3181 gcgcgatgag ttcatggccg ccgaaggcgc tggcgctcga atagacgaga agcttcatgc
     3241 gcagccctgc gactctgccg gcaagcgccg acgcaggaac cgggccggca cgccgcccca
     3301 gatctcgccc gccggaacga gcccgcgcac caccgcgccc gccgcgatca ccgcgccgtt
     3361 gccgacacgc gcgccgggca gtagcgtggc gcccgcgccg atccagacgt cgtcgccgac
     3421 gatgacggag tgcggcgcga cgctccaggg ctggtcgatc atcggcacgg ccggatccat
     3481 cccgtggttg gaagcgacca tcgtcaccat ctgcgcgatc aggcacttgc ggccgatgcg
     3541 gatctctccg ccgctcgcgc gcagattgca atactcgttg acggccgtgc ccgcaccgat
     3601 ccgcagacgc gacgcgtcag gcggcaaggc gctcatctcg gtcgtcgcca gcagcaggga
     3661 tccccatccg atgctcacgt ccgccgccag ctcgatgccc tgcgggcgat ggctgcgcag
     3721 caccacattg cgcgccagcc gcacaccacg ggcgcgccag cgcgcgcgca tccacatgtc
     3781 ccgcacggcg atggcgggcc cggtggcggc caggacacag aaatgcagca gggtcgcggg
     3841 catcgtgtca ggcgccagtc ccggcacaca catcggccag cgctgcccgg tacacgctgc
     3901 ggacgcggcc cgcgaggcct ccgccgtagc ggcgcgcgaa ttcgaactcg tcgtgcacgc
     3961 gcacgggcgt gggacagctg aggacagtgt ccgtcgtgtc gtgccgccgg tagaggaatc
     4021 ccacttcgtc gatgttgagg tagtttgagc gacgcagtac ctggcatacg aagtcgagat
     4081 ccgcgagcca tcgcagcgac gtgtcgaagc gcgcatcacg taccgcactg cggcgcagca
     4141 gcgtgttgct gggtgctccg actagattgg ccattcgacg ggcgcagcgc aggatagcga
     4201 catccccggg aagatggccc ggcagatagc tcgtgtcgta gcgcggattc aacatcgcgt
     4261 ccgtcacgcg acagttgcag ctgacgacgc cgacatcggg gtgcgcctcg agcgcggcga
     4321 tctgcttggc gagcatcccc ggaagcagca catcgtcggc cgacagcagc ttcacgtaat
     4381 ctgccgacgc caggtcgagt gcgcggttat ggttacgcaa ggaaccgaca ttggtatcgt
     4441 atgcaagaac ccgcacgccg cgatccgcat atccgcgcgc cacgcgcact gtgtcgtcgg
     4501 tcgaacggtt gtccaggacg atgatctcca tgccctcgac gctctgctcc aacgccgact
     4561 cgagcgtctg ggcaatgaag gcgcccgcgt tgtaggcagg aatgagcact tggacggtca
     4621 tggtgcagcg aagcgtttcc aatatcgacg aaagagcacg gaggcccacg gcaacgagac
     4681 gagcgctgtg gcgcagaaat agagttgggc ggtgagctcg acgctaaccc gcgatgccca
     4741 atgcgctatc gccagcgtaa ccgctgccag taccaccgac gcaagcatca tgggctcttc
     4801 cttgtgcgct cgcatgtaga cggcgaatgc aaagatcacc gagttcacca cggtcacgag
     4861 agccatccag cagacgactt cggtcgacga gatgcgatgt acgaaggtga agccggctcg
     4921 cgagagttcc catacgccaa ggacgaccgt tgcgctggcc aatgccgtga acgccgtcga
     4981 tggcttgagc gcacgcagga acgtccggtg caggtcctcg ccttcggcgc gcgaaatgtg
     5041 ggccgccatg cgggggctgc agctgtagac ccagctcatg ccgatcgact ggatggcgtt
     5101 gaacacggcc agcaccatgc cgaagcgccc cgcttccacg ggcccctggt tctggaacag
     5161 catgggcacg aacaactgga aaatgaaata gccgctcacc cagctgagcg cgatacgcca
     5221 ttggaacggc aagatctcgc gtcgccaatc aacgcgagcc gaggatgcca aggatcgcgc
     5281 gcgcagccac tcgatcatcc gcccaccgcg ccgaagccac agcgcactgc cggcagcggc
     5341 cgccacgggt accatggtcg acgcccaaag tgcgcagtgc gccgccagta cggcagccgt
     5401 cagagcgaat ccgagcacgg actgcatcag gcgcagccgc gcgacctcgc cgactcggcc
     5461 ggcgccctcg atcatcgcga ggctcggact gaggtagagg ttgcaggcgg tggccgaaca
     5521 catcagcagc cacggaccca gccactgcac gtccagcgac gatccgcgac gggagaagaa
     5581 tgcgacaccc gctgcgccga cgatcaccgc gaacgatccc gcggcaatcg cgtaccagcg
     5641 gcgcagcatg cgggcaagcg tcgtcagccg atcgattcgc tcgggctgcc cgctcagccc
     5701 gccgtccgcc gcgagtttca ggtgcgcgat ttcgtgcgac gtgatctggc tcacgacctg
     5761 gttcatgccc agctcgaaga aaatctgcag tgcaaggatg ctcgcgaacg tgtagtggta
     5821 gcccagctgt gccggtgtca gccatagcgg cagcaacacc agcaagctgg cgccggccag
     5881 cacggaccag ccgcgaaaca gcagagtcag gacgacgtgg aagtccaatc cgagcgcctc
     5941 gcgccaacgg gcgacgacgg aatgcggcgg cgatgcagac gaggtcatgc gggctgcgga
     6001 tgcgttcggt tcaagccgtc aggccgccgt gggcgttgag catcgcctgg gagccgaccg
     6061 tgccgcacac gcgtgggtcg agcgcctcgg cgcgcaggcg cggcaacagc tcgggcgagt
     6121 cgccgacggc ttccatgagc cacgagccga agccggcatc gcgcaggtga tcctcgaacg
     6181 tcgtcacgct cgcgcagcgc gcgacctgcg ccgcctgcgc gcccttcgct tccaggcccc
     6241 acaacggcag cgagtaaacc gcgggccgcg ggccggccgc cttgcggctg cgcgcgagcg
     6301 cgatgcccag tgccgcgccg gtggccagca acgtctcgcc gccgtcctcg tcgccggcct
     6361 cgacctgcag ccagcggccc ggctcgacgg ccggcacctc ggcatggaac ctcggctcac
     6421 cggccttgcc gaggcgcagg taggaaggcc caggattctc gaccagccaa cgcatgcatg
     6481 cccgcgtctc cagcgggtcg cccggtgcgg cgatggtcat gttgggcatc gagcgcatca
     6541 gcgcatagtc ttgcacggcg tggtgggagt accccagcgc gccgtaggcg aggccgccgc
     6601 cgaccgccac caccgtcacc gcgagtcggt gatagtcgac gtcgttgcgg atctgttccg
     6661 cgcagcggaa cgtcgggaag ttggcgatcg agtacgtgaa gacgtgatag ccctccgagg
     6721 ccatgccggc ggccagcccc gtcatgttct gctcggcgac gccggcattg atgaagcggt
     6781 cggggaagcg atcggcgaac ggttcgatga ccgaatagcc gagatcgccc acgaccagcg
     6841 cgacctgcgg atgcacggcc gcgagggcca ccagttcctc gatgaatgca tttctcatcg
     6901 ccgtccctca atcacccagc tcggacagag ccagcgcgag ctgctcatcg ctcggactgc
     6961 tgtaatgcca cttcacggcg ttctccatgt agctcacgcc cttgcccttg gtggtgtggg
     7021 cgatcaacac cgatggcttg ccgagcgtcc agggcgcggt gccgagcgcc tcggctagcg
     7081 cctcgtggtc gtggccgtcg acctcgcgca ccgtccagcc gaaggcggag aacttgtcgg
     7141 ccagcggctc gagggccagc gtctccgcca cggtcgtgag gctctgcaac ttgttgtagt
     7201 caacgatcgc caccaggttg tccaaccggt gatggctggc gaacatgcag gcttcccagt
     7261 tgctgccctc gccccattcg ccatcgctga ggacgacgaa ggttcgccag tcgccgccct
     7321 ggcgcttggc cgcgagcgcc ttgccgacgc cgaagggcaa tccgtgaccg agcgagccgg
     7381 tcgagaactc gacgcccggc accttgtggc tcacgtggtt catcagctcg gaatggtcct
     7441 ggccgtagcg cagcagctct tcctccgcga agaagccccg cgccgcgagc gccgcgtaga
     7501 ccgccacgca cgcgtggccc ttgctgagga tgaagcggtc gcggtcgggc gcgcgcgggt
     7561 cggcgggatc gatgcgcatc acgcggccgt agaggacggc gacgatgtcg gcgatcgaca
     7621 gcgcgctgcc gatatgggac gcgcgggcgc gatgcaccat gcgcagcgca tggcgccgga
     7681 tggtgccggc caggcgccgg gtgtcttcga tggattcggt catcgcaggg tctcgatcat
     7741 gcgcctgaaa ccctcgacgg gcgtcacttc ggcgcgccag ccgagcgcct cgaggcgtcc
     7801 cgtgtcaggc acgaggcggt tgtacgggct cgggatgtag ttcggactgt cggtgtcgac
     7861 gcgccggtcg acgcgcaggc gcttttccgg atagaggccg accagcaact cggccagttc
     7921 catcaccgac agctcggcgt cggcgttcgc cacgttgtaa gccgtccccg gcctgccgcg
     7981 cagcagcacg gcgaggaatc cggcgacggc gtcgctcgcg tagcagaagg cccgccgcgc
     8041 tgtgccgtcg ctgttcatca cgatgttctc gccgcgcgcc acgttgtagg cgaagtccgc
     8101 gaagacgcgg ccgtcctcgg ccgtcagccc aggaccatag gtatggaacg ggcgcacgat
     8161 ccaggtgggc aggccgtact ggtggtgcca ggccacgcag accgcttcgc cggcgcgctt
     8221 gccctcggcg tagcatgccc gcaccgtcgc gggatcgacc acgccgtagc cggtctcctc
     8281 gagcgtgccg ccgccggcga cagcgccata gacctcgctg ctgctgacga acatcaatcc
     8341 acgcggatcg ggcgctcggc gaagggccgc cagcagcgcc gtcgtgccga tcgtgttggg
     8401 cagcatcgtc cccaccgggt ccgtgccgta gaagcgcggg ctcgcctggc tggcggcgtg
     8461 caggacgtaa ttcacgtcgc cgagctcggg cacggcgatc gtgttcaggt cccactcgag
     8521 gaactcgagg cgtccggccg cgatgtcagc ggcgaggtcc gcgaatcgcg cacgtgcgcg
     8581 atccgcgtcg cgtaccagcg ccaggacgcg caccggctca tcgacgcgct gcaggcggtg
     8641 gagcgccagc agtgcgcgcg cgagatagcc gcccaggaag ccgccggctc cagtgacagc
     8701 cacccggcac cccgagagca tctgccaggg caggtcacgc gccgcgacgg cccgcaggtc
     8761 gccttcgacg atgtcgcgca tcgtgtcgtc catcgtccgt cctcagaagt tgagcccgaa
     8821 gaactcttcg agcttgtcga cgacgtagtc cagctgctcg cgcgacaggc ccggccagac
     8881 gcccagccag aaggtctggt tcatcacgat gtcggtgttg cgcagctcgc cgctgacgcg
     8941 gaagttgcgg ccgatcatgt agggctggcg cgtgaggttg ccggcgaaca gcaagcgcgt
     9001 ggcgatcttg ttctggtcga ggaagttgat caggtcggcg cgcttggcgc cggccgtctc
     9061 cttcaggatc accgggaagc cgaaccagga cgggtcggac ttcggcgtcg cctcgggcag
     9121 gtgcaggaac tcggcgacac cgccctgctg caggcgctcc ttcaggtagg cgaagttggc
     9181 cttgcgcgcg gcgatgaagt cctcgacgcg atccatctgc gccagcgcgc aggcggcctg
     9241 catgtcgctg atcttcaggt tgtagccgag gtggctgtac gtgtacttgt ggtcgtagcc
     9301 ctcgggcagg tcgccgagct tccagcagaa gcgcttgttg caggtgttgt ccttgccggg
     9361 cgggcagtag cagtcgcggc cccagtcgcg gaaggactcg gcgatcagct tcagctcggg
     9421 atcgttggtg aagaccgcgc cgccctcgcc catcgtgatg tggtgcgcgg ggtagaagct
     9481 gagcgtgccg atgtcgccga aggtgccgac gagcttgccg tcgtaggtcg cgccgagcgc
     9541 gtcgcagcag tcctcgacga gccacagccc gtgcttgcgg cagatcgccg tgacgacctc
     9601 gaggttgaac gggttgccca ggctgtgggc cagcatgatc gccttggtct tcggcgagat
     9661 cgcggcctcg atcaggctcg cgtcgatgtt gtgcgtggcg aggtccacgt ccacgaacac
     9721 cggcaccgcg ccgaactgca ggatcgggtt gacggtggtc gggaagcccg cggccacgcc
     9781 gatgacctcg tcgcccggcc tgatcgcgcg ctcgcccagg cgcggcgagg tcagcgtcga
     9841 gaacgcgacc aggttggccg acgagcccga gttgaccgtg atgaggtgct tgacgccgag
     9901 gaacttcgcc agccgctcct cgaacatcgc gttgaagcgg cccgtggtca gccagccgtc
     9961 caggctcgcg tcgaccatca tcttcagctc gcccgcgccg atcaccttgc cggagaccgg
    10021 caccgggctc tcgccgggca cgaacgggcg cggcgcgtgg acgatgtccg agtactgctg
    10081 caccagcgcg gcgatctccg cccgcagctg gtcgggcgtc ttcttgtgga actggacggg
    10141 ggtctcggtc atggtgcggg gctcgtgagg gcttcggagt actgctggat ctggcgcagg
    10201 cacagcgcgc ggacgtcgtc gccggcgagc caggcgcggt gccattcgac gatgcgcgcg
    10261 agcgcctcgt ccaggcgcca gcgcggctgc cagccgaggc gcgcgcgcgc cttggagatg
    10321 tcgagcttga ggaagtgggc ctcgtgcgga tgcgcggcgg cgtccaggcg ccagcccgcg
    10381 ccctcgcccc aggcggtggc caggcgctcg acgatccagc gcaccgggcg cgcgtcctcg
    10441 tcgtgcgggc cgaagttcca gccctcggcg acgctcgcgc ccttctgcca caggcgctgg
    10501 gcgagcgtga ggtagccgga cagcggctcc agcacgtgct gccagggccg cgtcgacgac
    10561 gggttgcgga tcacgaccgg ctcgccgcgc tcgaacgcgc gcaggatgtc gggaacgagg
    10621 cgatccgtcg cccaatcgcc gccgccgatc acgttgcccg cgcgcgcgga ggccagcgcc
    10681 acgccggcct gctgcaggaa cgagcggcgg taggcgctcg tgaccagctc cgcgcagccc
    10741 ttgctgttgc tgtagggatc gtggccgccc atgggttcgt cctcgcgata cccccaggcc
    10801 cattcgcggt tctcgtagca cttgtcggtc gtcacgcaga cgacggcgcg cacgccgggc
    10861 gcgcggcgca gcgcctcgag cacgtgcacc gtgcccatga cgttggtcgc gtaggtctcg
    10921 acgggctcgg cgtacgacag gcgcaccagc ggctgcgcgg ccatgtggat cgcgatctcg
    10981 gggcggaagg cctcgaccgc gcgccgcacg gcgtcgaggt cgcggatgtc gccgagggtg
    11041 ctggccatgc cctcggccac gcgcgcggca tcgaacagcg cgggctcgga cggcggcgcg
    11101 agcgcgaagc cctgcacctg cgcgcccagc tgctgcagcc acagcgcgag ccagctgccc
    11161 ttgaagccgg tatggccggt caggaagacg cgcttgccgc gccagaaatc gacatcgacc
    11221 gtcacggcgc cgtcactccc acgtcttcca cggcgccttg cccgaggccc agagctcctc
    11281 gaggtagacc ttgtcgcgca gcgtgtccat cggctgccag aagccctcgt actggaacgc
    11341 catcagctcg ccgcggtcgg ccaggttcat cagcggctgc tgctcccaga cggtatcgtc
    11401 gccgtgcagc tcctcgagca ccttcggcga gagcacgaag aagccgccgt tgatcatggc
    11461 gccgtcgccc ttgggcttct cctggaagct gtggacgcgg ccctcgcgga tgtcgagcgc
    11521 gccgaagcgg cccggcgggt acgtcgccgt cagcgtggcg gccttgccgt gcgccctgtg
    11581 gaaggcgatg gaggccgaga tgtcgagcgc gctcacgccg tcgccgtacg tgaagcagaa
    11641 ggcttcctcg tcctcgacgt acttggccac gcgacgcagg cggccgccgg tcatcgaggt
    11701 gtcgccggtg tccacgaggg tcacgcacca gggttccgcg cgcttgctgt gcacttccat
    11761 cttgttgtgc gccatgtcga aggtcacgtc cgacatgtgc aggaagtagt tcgcgaagta
    11821 ctccttgatc aggtagccct tgtagccgca gcagatcacg aagtcgttga tgccgtggtg
    11881 cgaatacatc ttcatgatgt gccacaggat cggcttgccg ccgatctcca ccatcggctt
    11941 cgggcggatg gccgtctctt cgctcagccg ggtgccgagt ccgccggcca ggatcactgc
    12001 cttcatgtgc gtgcttctct tcgcgaggtt ggtcgggagt ccccgcgtca gtcggagctg
    12061 acgaaggcgt cgtagtggaa ccgcgcgggc ggcaggccgt tgtcggcgag caggcgccgc
    12121 gcgccgtgga tcatggcctc ggagccgcag gcgtagaccg cgcagccggc cagcggcggc
    12181 gcggcgccgt gcgcgacgtc gtgcaccagc gcgt
//
LOCUS       DASVLN010000479        15699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_455293, whole genome shotgun sequence.
ACCESSION   DASVLN010000479 DASVLN010000000
VERSION     DASVLN010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15699
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455293"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /gene="epsE"
                     /locus_tag="VF453_11780"
     CDS             <1..766
                     /gene="epsE"
                     /locus_tag="VF453_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HEX7688383.1"
                     /translation="STSAAAPAGVAVDQSRIGPGDTIRITVYQSPDLSMETRVNENGF
                     ISYPLLGSVKMSGYTVEAAEQHLAQELKRHDFVKDPQVNIVVTQVRANQVNVLGQVGH
                     PGRYPLDLAGMRMTEVLALAGGVVAGAGSDTVVLVGERDGKPYRHEVDLPRIFAPGGR
                     GDDIVLMPGDTIWVDRAPQIYMYGEIQHPGTMRLERGMTVMQAVAAGGGATPRGTLKG
                     LRVTRRGADGRMQTFEPSMEDSLKDGDVVFIRESLF"
     gene            780..2207
                     /locus_tag="VF453_11785"
     CDS             780..2207
                     /locus_tag="VF453_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX7688384.1"
                     /translation="MNLAHFLAVLRARWIAALAAFLVVLLAAIGYLTFATRVYEATAT
                     ILIDAKPDPVSSLLYGGATPALVNTQLEIIRSDRVAQRVVQNLKLADTADMRAAWASA
                     KTGATLQDWLVNFIENGLDAKVTTAGANVISVGYRSADARFAATAANAYVQAYLETSI
                     ELRVDPAKQYSGFFNDQQKAARDALEAAQNKLAAFQREKGIIGTDDHLDMELNRLNLL
                     TQELVTVQQERVAASTRQSQVGNASQMAEVLQNGNVSSLKQELATAELKLQDLSSRYG
                     DKHPQVQDARADVNALRAKIAAATRDVTGAVGIDARASRAREAEIQAELEAQRAKVLQ
                     LKDVRDQASVLQRDVDSAQHNYDMVNNRASQTALESQNRQATSTVISTATPPAEPSSP
                     KLVAGLLMGIVAAIGAGLGVALLLEQLDKRVRTTSDAFDFLGLPVIGIMPSPSMNRRL
                     KGQMAMIQGRVISGRRLTGPTKGQA"
     gene            2204..3106
                     /locus_tag="VF453_11790"
     CDS             2204..3106
                     /locus_tag="VF453_11790"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX7688385.1"
                     /translation="MNDKNEGPDLVLGEGQPNDGGPRAKAIGELIRDARNLSADDIER
                     IGRYQRENDLRFGEAAIALGLADTEDVLQALAQQFKYAYADEETRKAAPELVMLNQPF
                     GHQAEAFRAIRSQILMRTQTEAKGQRRPLAVVSPDSGDGKTFFCANLAVALAQLGGKV
                     VVVDADMRGARMDQVFGVPGSSGLSGVLSGRGGKGVVKSVPGIPNLYVIPVGAQPPNP
                     LELVEGPSFGMLLREISIKFDHVIVDTPAACYGSDGVVIAARCGSAIVVTRKDANKVG
                     PLQELVASLQSGQVRLAGVIMNEF"
     gene            3115..4023
                     /gene="xrtB"
                     /locus_tag="VF453_11795"
     CDS             3115..4023
                     /gene="xrtB"
                     /locus_tag="VF453_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase B"
                     /protein_id="HEX7688386.1"
                     /translation="MDLSEIRATAHEAWRAMSAPRYRAWWIALLGVLCLYTGTFYGLA
                     TTIWTSEEQSQGPIVLAICVWLVWRAWPRIDDSQADAPRPVAGWVLVGFALLLHVFGR
                     SQRILSAQTLSLVFMLPGIVLLLRGRKQVAAAWFPLFFMLFLVPLPSTMVDAITQPLK
                     LAVSSVATNVLSAIGYPIARTGVIMQIGQYQLLVADACAGLHTLFSLEAMGLLYLNVV
                     RHTSWLRNIALAILIVPISFTANVIRVMVLALITYHFGDEAGQGFLHGFAGMVLFMTA
                     LVFIIATDSALRWLAVRHAPAQPHHA"
     gene            4016..4708
                     /locus_tag="VF453_11800"
     CDS             4016..4708
                     /locus_tag="VF453_11800"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HEX7688387.1"
                     /translation="MPDNVLNRRAAIAAILMAGAAVAGEAMVPTRKMARLRGHFDIEE
                     LVPRSFGDWRIDTHAVGGIVNPETAAMLNRIYSQLLDRVYIDSTGDRIMLSIAYGDDQ
                     ADDSMQLHYPEVCYPAQGFQVKANRREQISTPEGLIHVRRLETQQGEMRYEPVTYWTI
                     VGDQQVIGGWERKKSEILHGIQGEIVDGLLFRVSSIDRNAKEGFESQDRFIRDVVKAM
                     SPAARRQLVGLA"
     gene            5091..7289
                     /locus_tag="VF453_11805"
     CDS             5091..7289
                     /locus_tag="VF453_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688388.1"
                     /translation="MQRFLPPGLALSSIAVVLSTTLAACGGGSTDTSSAASTQSTTDS
                     SASTTASTDASIDTGTVASTVDTNAQDGSAATAMADAAANDAATAKPIISSSDLAGTT
                     VTQAGTTATAGTGGTTSGTTAAATAGAQSSGSDSVAPASSGLASIGSGTPGDTTRKAS
                     GAGLNLSAFNYFSPEIPTFDVMKRGSPWLTQCSTSTLCANFTGGAGTWETLEESKLPL
                     DANGYPTSLPAANDTTVKYRKVTTMLSSNGSLPPGQYTIVYDGAGTLAYGGSVSIVSS
                     KPGRDVVNLAAGSTSSFWLSITATSASNHLRNIRVYLPGGACANDLTTYAASASACTA
                     SQGAFVPFESFPAGSTWNPQFLTDVKGFRTLRFMDWGQTNSTALSSWSNRPLITDRTW
                     TSANGVPLEAMFDLANKTSADPWINIPPHADDNYITQAAKVALQAVDASHKINLEYGN
                     EMWNYSFPATKWALAQAQAAWPAEVAAGANVYELESNWYARRLVQACTLAKGAGTGAA
                     SRFTCISNTQASVATQTDQVLRCTYAAKTLGQPCAKFIDVAAVAPYFGQYTNGTAFRP
                     TVTTWYALADGGLSNLFAELTGTDANGTPIVKSALSLVNSAAPAGGALSQLKGWMQQS
                     QAIAAKYGLPLWTYEGGQGLVPQTNDTDTHFINLTIAANRDARMGQAYQRMFSDWQAV
                     GGQVFTYYSHVATPGKFGSWGAKETMTDNGNAKWVAVKQARDTACWWTGC"
     gene            7620..9062
                     /locus_tag="VF453_11810"
     CDS             7620..9062
                     /locus_tag="VF453_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX7688389.1"
                     /translation="MTQAADTLPPGAEGAAEAPAPTVKPAALKGRVLRAGKWTLGSQL
                     VAQFLRLATNVVLARLLFPDAFGLMAVVNMLITALVLFSDIGINRAVIQSRRGDDPRL
                     LDTAWTVQVWRGFGLAGACLAAAAGIALGAHLHLFRAGTVYADPRLPWLIVVFAITPA
                     IQGFDSICIAQSKRQMNLHTLTKIELAGQLASAVVMIVIAYATRTVWTLVFGAMAAAA
                     VRTTLGHRLLPGYRARFRVDKDALDEILASGKWIFLSSIMSFLAMNSDKVLLGGLIDA
                     RAFGLYSIALLMANVLQGLATKMCMSIVYPALAEVHRDRPQDLAKTIAKFQWGYDALV
                     TSLAALLITAAPALIGLMYDHRYHDAGWMLSVLAVGVIGLRFQIVEECYQAVHKPQYA
                     TFANLIRLCTLAGGIFIARHLWGMTGAIAAVAVTTFAAWPLAIWFKVRQHVFTWRAEA
                     LLVPAIVGGLACGWVLRQVIEWAAPGRFLG"
     gene            complement(9109..10083)
                     /locus_tag="VF453_11815"
     CDS             complement(9109..10083)
                     /locus_tag="VF453_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688390.1"
                     /translation="MTQDTAPARRKAYCVMGAGSLPYARRSLESLCRHSMDALDVRII
                     TDEIEDKARITAAMQELGIEARHAWSVHTKAELDAAADDRFAAFPELKHLREGHPCWR
                     KITDPLLLAEPDEEILLLDPDLYFPNRFRFEPTPARGVLLMRQRPHCLLPHETVMNAY
                     DGGVALAHHVDIGVAQLRHNLDLGWMNAMVAAMRRGGELPRKMHIEAIIWSGVALREG
                     GGYLDPTHWHCWQNSQWKRLALKLGISGVRVLRSDDFSKMKCFHGGGPAKWWIPGFID
                     AGYMPAPGIVDAHAKPQPFVPLTRETYLSGQRWKSLARRLGYYRLVKA"
     gene            complement(10133..12466)
                     /locus_tag="VF453_11820"
     CDS             complement(10133..12466)
                     /locus_tag="VF453_11820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7688391.1"
                     /translation="MNSHRVNALDGWRGISISLVLAGHLLPLGPKSWLLNGAVAGCGM
                     AIFFTLSGFLITSVLLRDDDIPNFLVRRLARIVPLAWLAALIALTATRAPLADYLPHL
                     LFIANTGPIWLTSITGHYWSLCVEVQFYLGIALLVGLFGRRALPVLPLLALAVTALRA
                     SRHELMAIDTQYRIDEILAGCTLALVFDRLRAAPPSPRLGSLSWLLLPLLVASAHVDA
                     GALNYLRPYIASTMVGLSLFAAPQSRTARLLSARVLQWLARISYAVYIIHGCLMFSWL
                     GEGATPLVRYAKRPLFFLVTFALAQLSTDHYESRFIAWGKRWTQARDRRLGRGGARHE
                     EATRIPAPIDAAPDAPKAMGILIVSEHASTRFGGEAALAFHYFRVMRARGLDVRLLTH
                     ARVRDELLARFPEDRDRLLFVEDTPAQRLLWRLGRKLPARVASFTTGFVSRGITQRAQ
                     RAIARRLVAEGRVRVVHQPMPVSPREPSLIADVGAPVVIGPLNGNMQFPPAFRRREGA
                     LVDLVERGARAFTGLMNALFPGKRRAAAVLVANERTGRGLPGGLSGQVLHLVENGVDM
                     SLWGAPSGAGQASDPAQAPAATRFVYMGRLVDWKAVDLLLHAFERARGRAPMTLTLIG
                     DGPERPALQALAGTLGLRDDGPGVPGRLHFAGWMSQADCARELQCSDVLVLPSLWECG
                     GAVVLEAMACRMPVIATAWGGPLDYLDEHCGVLVPPESRERLVQGFADAMVALASEPE
                     RRRAMGAAARARVEAGFDWERKVDRILDIYRDVVARA"
     gene            12772..14385
                     /locus_tag="VF453_11825"
     CDS             12772..14385
                     /locus_tag="VF453_11825"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7688392.1"
                     /translation="MVYAIKLLFAVLLLSAVFAPFLRGAMRGLIGDKDYWRVWMIIVA
                     TALASFLSMRPPIFMFLVAMIGLFAPRLLGGGTLGRLTAWWLLYVLFPPISMSAEGLP
                     GINRIIELMAHRVLSLVVLVPAVFELLGNKQVRANRMTRWTDLAFIAYPTMLILTAIP
                     SSSSTNVARSVVEMALDLLLPYWVTTRAIRSAADLKHLCVRLVIGLAFLAAVGLMESA
                     LRKNVYSELQFIVGVIWQATHRLMRGPFLRVQATFPVPIVMAYVMTIGFCLWSWLGRT
                     STTTRRMRLLVSFGFFLAMAATFSRGPLLGWASFVITLIALRWISPYKYLAGLTLLFG
                     VAVGASVTGTDEWAYEALKTVFGSSAADTSSIDYRRRLLETAVALIKQSPTWGVPNYM
                     AYMQDLRQGEGLIDIVNTYVAVALANGIVGLVLFMSPHVVVLVRLLRRLNSYEERPRR
                     SVGAFPATMVAATVASLLTIFTTSDFSAVPRMLLFLIAVPTAWLAFDRAEQRRADEVV
                     PVGDEVAPRGHPRFAPIPQEPGWREPNWQ"
     gene            14382..14924
                     /locus_tag="VF453_11830"
     CDS             14382..14924
                     /locus_tag="VF453_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688393.1"
                     /translation="MSADGLPAPVHAAISASDPDWGREARTSFWQPSRALLQSIRAYQ
                     RHAGSRSPMAAAARGLAVLRHRFWSVVTGADIPLNCRIGGGLLLPHPNGVVVHPSARL
                     GPNCLLFQQVTIGAGGRPGFPVIGGHVDIGAGAKVLGGVTVGDHARIGANAVVTRDVP
                     AHATAVGIPARVVATAGADE"
     gene            14983..>15699
                     /locus_tag="VF453_11835"
     CDS             14983..>15699
                     /locus_tag="VF453_11835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7688394.1"
                     /translation="MTRHDDPGASTSSPLAGMGIVVIGRNEGERLVRCLRSVAAVAGR
                     VVYVDSGSTDGSVAAARAAGADVVELDLRTPFTAAKARNAGWQRLQQSLPQLSSVHFL
                     DGDVEVVPGWLETAAAELARHPEAAVVCGRRRERHPERSVFNLLCEIEWNAATGEVRA
                     CGGDALMRLDALRAVGGYRPGLIAGEEPELCVRLRAAGWKIRRLPQDMAWHDAAMTRW
                     SQWWQRSKRAGHAFAEGASLH"
BASE COUNT         2364 a         5550 c         5501 g         2284 t
ORIGIN      
        1 ttccacctcg gcggccgcgc ccgccggcgt ggcggtcgac cagtcgcgca tcggccccgg
       61 cgacacgatc cgcatcacgg tctaccagag ccccgacctg agcatggaga cccgcgtcaa
      121 cgagaacggc ttcatcagct accccctgtt gggcagcgtg aagatgtccg gctacaccgt
      181 cgaggccgcc gagcagcacc tggcgcagga gctcaagcgc cacgacttcg tcaaggatcc
      241 ccaggtcaac atcgtcgtga cccaggtgcg cgcgaaccag gtcaacgtgc tcgggcaggt
      301 cggccatccg ggccgctatc cgctggatct cgcggggatg cgcatgaccg aggtgctcgc
      361 gctcgcgggc ggggtcgtcg ccggcgcggg ctcggacacc gtggtgctcg tgggcgagcg
      421 cgacggcaag ccctaccgcc acgaggtcga cctgccgcgc atcttcgcgc ccggcggccg
      481 cggcgacgac atcgtgctga tgccgggcga cacgatctgg gtcgaccgcg ccccgcagat
      541 ctacatgtac ggcgagatcc agcaccccgg caccatgcgc ctggagcgcg ggatgacggt
      601 gatgcaggcg gtcgcggccg gcggcggcgc cacgccgcgc ggtacgctca aggggctgcg
      661 cgtgacgcgc cgcggcgccg atggccgcat gcagaccttc gagccctcga tggaggactc
      721 gctcaaggac ggcgacgtcg tcttcatccg cgaaagcctg ttctgaggac gctcctgcca
      781 tgaatctcgc ccacttcctc gccgtgctgc gcgcgcgctg gatcgccgcc ctggcggcct
      841 tcctcgtcgt gctgctggcg gccatcggct acctcacgtt cgccacccgc gtctacgagg
      901 ccaccgccac gatcctgatc gatgccaagc ccgatccggt gagctcgctg ctgtatggcg
      961 gcgcgacgcc ggccctggtc aacacgcagc tggagatcat ccgcagcgac cgcgtggccc
     1021 agcgcgtggt gcagaacctg aagctggccg acaccgccga catgcgcgcc gcctgggcct
     1081 cggccaagac cggcgcgacg ctgcaggact ggctggtcaa cttcatcgag aacggcctcg
     1141 acgccaaggt gacgaccgcg ggcgccaacg tcatctccgt gggttaccgc tcggccgacg
     1201 cgcgcttcgc ggccacggcc gccaacgcgt acgtgcaggc gtatctcgag acctcgatcg
     1261 agctgcgcgt cgatccggcc aagcagtact cgggcttctt caacgaccag cagaaggccg
     1321 cgcgcgacgc gctggaggcg gcgcagaaca agctcgcggc gttccagcgc gagaagggca
     1381 tcatcggcac cgacgaccat ctcgacatgg agctgaaccg cctgaacctg ctgacgcagg
     1441 agctcgtgac ggtgcagcag gagcgcgtcg cagcgtccac gcgccagagc caggtcggca
     1501 acgcgagcca gatggccgag gtgctccaga acggcaacgt cagcagcctg aagcaggagc
     1561 tcgcgaccgc cgagctgaag ctgcaggatc tgtcctcgcg ctacggcgac aagcacccgc
     1621 aggtccagga cgcgcgcgcc gacgtcaacg cgctgcgcgc caagatcgcg gccgccacgc
     1681 gcgacgtgac cggggcggtc ggcatcgacg cccgcgccag ccgcgcgcgc gaggcggaga
     1741 tccaggccga gctggaagcc cagcgggcca aggtgctgca gctgaaggat gtgcgcgacc
     1801 aggcgtcggt gctgcagcgc gacgtcgaca gcgcccagca caactacgac atggtcaaca
     1861 accgcgcctc gcagacggcg ctggagagcc agaaccgcca ggccacgtcg acggtcatca
     1921 gcacggccac gccgcccgcc gagccgtcgt cgccgaagct ggtcgccggc ctgctgatgg
     1981 gcatcgtcgc cgcgatcggc gccggcctgg gcgtcgcgtt gctgctcgag cagctcgaca
     2041 agcgcgtgcg cacgacctcc gatgcgttcg acttcctcgg cctgccggtg atcggcatca
     2101 tgccgagccc gtcgatgaac cgccgcctga aaggccagat ggcgatgatc cagggacgcg
     2161 ttatctccgg ccgcaggctg accgggccga ccaaagggca agcatgaacg acaagaatga
     2221 agggccggac ctggtcctcg gcgagggaca accgaacgac ggcggcccgc gcgccaaggc
     2281 gatcggcgag ctgatccgcg acgcccgcaa cctgtccgcg gacgacatcg agcgcatcgg
     2341 ccgctaccag cgcgagaacg acctgcgctt cggcgaggcc gccatcgcgc tgggcctggc
     2401 cgacacggag gacgtgctgc aggcgctcgc gcagcagttc aagtacgcct acgcggacga
     2461 ggagacccgc aaggccgcgc cggagctggt gatgctcaac cagcccttcg gccaccaggc
     2521 cgaggcgttc cgcgccatcc gcagccagat cctcatgcgc acgcagacgg aggccaaggg
     2581 ccagcgccgt ccgctcgcgg tcgtcagccc ggacagcggc gacggcaaga ccttcttctg
     2641 cgccaacctc gcggtcgcgc tcgcgcagct gggcggcaag gtcgtcgtgg tcgacgcgga
     2701 catgcgcggc gctcgcatgg accaggtgtt cggcgtgccg ggcagcagcg gcctgtcggg
     2761 cgtgctctcg ggccgtggcg gcaagggcgt cgtgaagtcg gtgcccggga tcccgaacct
     2821 gtacgtgatc ccggtcggcg cccagccgcc caacccgctg gagctggtcg agggcccgtc
     2881 cttcggcatg ctgctgcgcg agatctcgat caagttcgac cacgtcatcg tcgacacgcc
     2941 ggctgcctgc tacggcagcg acggcgtcgt gatcgcggcg cgctgcggct cggcgatcgt
     3001 cgtcacgcgc aaggacgcca acaaggtcgg accgctgcag gagctcgtgg cgtcgctgca
     3061 gtccggccag gtgcggctgg cgggcgtgat catgaacgag ttttgagccg agccatggac
     3121 ctgtcggaga tccgcgcgac ggcgcacgag gcgtggcgcg ccatgtccgc gccgcgctac
     3181 cgcgcctggt ggatcgcctt gctcggggtg ctgtgcctct acaccggcac cttctacgga
     3241 ttggcgacga cgatctggac cagcgaggag cagagccagg gcccgatcgt gctcgcgatc
     3301 tgcgtctggc tggtctggcg cgcctggccg cgcatcgacg acagccaggc cgacgccccc
     3361 cgcccggtgg cgggctgggt gctggtcgga ttcgcgctgc tgctgcatgt cttcggccgc
     3421 tcgcagcgca tcctgagcgc gcagacgctg tcgctcgtgt tcatgcttcc gggcatcgtg
     3481 ctgctgctgc gcgggcgcaa gcaggtcgcc gccgcctggt tcccgctgtt cttcatgctg
     3541 ttcctggtgc cgttgccgtc gacgatggtc gatgcgatca cccagccgtt gaagctcgcc
     3601 gtctcgagcg tcgccacgaa cgtgctgtcg gccatcggat atcccatcgc gcgcaccggc
     3661 gtgatcatgc agatcgggca gtaccagctg ctggtcgccg acgcctgcgc ggggctgcac
     3721 acgctgttct cgctcgaggc gatgggcctg ctgtatctca acgtcgtccg ccatacctca
     3781 tggctgcgca atatcgcgct ggcgatcctg atcgtgccga tctccttcac ggccaacgtg
     3841 atccgcgtga tggtattggc gctgatcacc taccatttcg gcgacgaggc cggtcagggc
     3901 ttcctgcacg gtttcgccgg catggtgctg ttcatgacgg cactcgtctt catcatcgcg
     3961 accgacagcg cgctgcgctg gctggccgtc cgccatgcgc ccgcgcagcc gcaccatgcc
     4021 tgacaacgtc ctgaaccggc gcgcggccat cgcggcgatc ctcatggcgg gcgcggccgt
     4081 cgcgggcgag gcgatggtgc cgacccgcaa gatggcccgg ctccgcggtc atttcgacat
     4141 cgaggaactg gtgcccagga gtttcggcga ctggcgtatc gatacccatg cggtcggggg
     4201 tatcgtgaat cccgaaaccg cagcaatgct taaccgcatt tacagccagt tgctggaccg
     4261 tgtatatatc gattcgaccg gcgatcgcat aatgctttcc atcgcgtacg gcgacgacca
     4321 ggcggatgac agcatgcaat tgcactatcc cgaggtctgt tatccggcgc agggtttcca
     4381 ggtgaaggcc aaccggcgcg aacagatctc gactccggaa ggcctcatcc acgtccgccg
     4441 gctggaaacg cagcagggcg agatgcgata cgagccggtg acctactgga cgatcgtcgg
     4501 cgaccagcaa gtgattggag gatgggagcg caagaaatcc gagatcctgc acggcatcca
     4561 gggtgagatc gtcgacgggc tgctgttccg tgtctcctcg atcgatcgca atgccaagga
     4621 aggcttcgag tcccaggatc gcttcatccg cgacgtcgtg aaggccatgt cgcccgccgc
     4681 ccgccgccaa ctcgtcggcc tggcctgacc ccgcgccgcc cgcgtgcggc gcctgcaagc
     4741 agccccatcc gacgacggcc gcctagccgt cgcgggcgcc cctgtcccga cggcagagcg
     4801 ggataaggtc gagctacaac tcgatacata ctcggctaca actgaaacat attcatcgag
     4861 gtcgacatat tgatcgtcct cgtagggttt tatcgatttc tcgtcgcatt gcgtttcgag
     4921 aattcggaca cgtttccttt ctttaattga tctggacgcc cttcagggcg cttatcgaac
     4981 tcaaacagta catcgggtcc gccgaagaac tgggctgaac cgcgggatcc agcgagacaa
     5041 gactcgccgg cgaacggccg cgggttcccc ttcttcttcg agactccgaa atgcaacgtt
     5101 ttctgccccc cgggctcgcc ctttccagca tcgcggtcgt gttgagcacg acgctggcgg
     5161 cgtgcggggg tggttccacc gacacgtcgt ccgcggcctc cacgcagtcg acgaccgact
     5221 cgtcggcgtc caccaccgcg tcgaccgacg cttccatcga cacgggcacc gtcgcctcga
     5281 ccgtcgacac caacgcgcag gacggcagcg ccgccacggc gatggccgat gccgccgcca
     5341 acgacgcggc gaccgccaag cccatcatct cctcgagcga cctcgccggc acgacggtga
     5401 cgcaggccgg cacgaccgcc acggcaggca ccggcgggac gaccagcggc acgaccgctg
     5461 ccgccaccgc cggcgcccaa tccagcggct ccgattccgt cgcgcccgcg agttccggcc
     5521 tggccagcat cggcagcggc acgccgggcg acacgacgcg gaaggcctcc ggcgccggcc
     5581 tgaacctcag cgccttcaac tacttctcgc ccgagatccc gacgttcgac gtgatgaagc
     5641 gcggttcgcc gtggctgacc cagtgcagca cctcgacgct gtgcgcgaac ttcacgggcg
     5701 gcgcgggcac ctgggagacg ctggaggaaa gcaagctccc gctggacgcc aacggctacc
     5761 cgacgagcct gccggcggcc aacgacacca ccgtcaagta ccgcaaggtg acgacgatgc
     5821 tgtcgtccaa cggttcgctg ccccccggcc agtacacgat cgtctacgac ggcgccggca
     5881 cgctggcgta cggcggctcg gtgagcatcg tctcgtccaa gcccggccgc gacgtcgtga
     5941 acctcgcggc gggcagcacc agctcgttct ggctgtcgat cacggcgacg agcgccagca
     6001 accacctgcg caacatccgc gtctacctcc ccggcggcgc ctgcgcgaac gacctgacga
     6061 cctacgccgc ctctgcctcc gcctgcaccg ccagtcaggg cgccttcgtg ccgttcgaat
     6121 ccttcccggc cggcagcacg tggaacccgc agttcctgac cgacgtgaag ggcttccgca
     6181 cgctgcgctt catggactgg ggccagacca acagcacggc cctgtcgtcg tggagcaacc
     6241 gtccgctcat caccgaccgc acctggacga gcgccaatgg cgtgccgctc gaggccatgt
     6301 tcgacctggc caacaagacg tccgcggatc cctggatcaa catcccgccg catgcggacg
     6361 acaactacat cacgcaggcc gcgaaggtcg cgctgcaggc cgtcgacgcg agccacaaga
     6421 tcaacctcga atacggcaac gagatgtgga actactcgtt cccggcgacc aagtgggcgc
     6481 tcgcgcaggc ccaggccgcc tggccggcag aggtggccgc gggggcgaac gtctacgagc
     6541 tcgagagcaa ctggtacgcg cgccgcctgg tgcaggcctg cacgctcgcc aagggtgccg
     6601 gcacgggcgc cgcgtcgcgc ttcacgtgca tctccaacac ccaggcctcg gtcgcgacgc
     6661 agaccgacca ggtgctgcgc tgcacatacg cggccaagac gctgggccag ccgtgcgcca
     6721 agttcatcga cgtcgccgcg gtcgcccctt acttcgggca gtacacgaac ggcacggcct
     6781 tccggccgac cgtcacgacg tggtacgcgc tggccgacgg tggcttgtcg aacctgttcg
     6841 cggagctgac cggcaccgat gccaacggca cgccgatcgt gaagtcggcg ctgtccctcg
     6901 tgaattcggc ggcgccggcc ggcggtgcgc tgtcgcagct caagggatgg atgcagcaga
     6961 gccaggcgat cgcggccaag tacggcctgc cgctctggac gtacgaaggc ggccagggcc
     7021 tcgtgcccca gaccaacgac accgacacgc acttcatcaa cctgacgatc gccgccaacc
     7081 gcgacgcacg catggggcag gcctaccagc gcatgttcag cgactggcag gccgtcggcg
     7141 gccaggtgtt cacgtactac agccacgtgg cgacgccggg caagttcggc agctggggcg
     7201 cgaaggaaac catgaccgac aacggcaatg ccaagtgggt cgcggtcaag caggcgcgtg
     7261 acaccgcgtg ctggtggacg ggctgctgat ccccgcgacc gcggccgcgc agggccccga
     7321 aacggggcgc cgcgcggcgc tgtcgatgcg atagactcga cggccgcccg atcccagcga
     7381 tcgggcggct tctttcttgc tacagaggct ttcccgctac tccgcgaccc gcagatgact
     7441 gcaaccctct cggcagccgg tgcgtcggtc tcgcctcgac aggcgacctc gccggtccgc
     7501 caccacgccc cccgatgcgt tccggcagcg ctcgcgcccg gcggcgcgga cgctcattcc
     7561 ggccagcgcg cgcgtaacga ctgccgcagc ctcggcggca ccacgcagcg gagccgcgca
     7621 tgacgcaagc cgccgacacc ctcccgcccg gcgccgaggg cgcggccgaa gcgcctgccc
     7681 cgaccgtcaa gccggccgcg ctcaagggtc gcgtgctgcg cgccggcaaa tggacgctgg
     7741 gatcgcagct ggtcgcgcag ttcctgcggc tggcgaccaa cgtcgtcctc gcgcgcctgc
     7801 tgttccccga cgcgttcggc ctgatggccg tcgtgaacat gctgatcacg gcactggtgc
     7861 tgttcagcga catcggcatc aaccgggcgg tcatccagag ccgccgcggc gacgacccgc
     7921 gcctgctcga caccgcctgg acggtgcagg tctggcgcgg cttcggcctg gccggcgcgt
     7981 gcctcgccgc cgcggccggc atcgccctgg gcgcgcacct gcacctgttc cgcgccggca
     8041 cggtgtacgc cgacccgcgg ctgccgtggc tgatcgtcgt gttcgcgatc acccccgcga
     8101 tccagggctt cgattcgatc tgcatcgcgc agtccaagcg acagatgaac ctgcacacgc
     8161 tgaccaagat cgagctcgcc gggcagctcg cgtcggcggt ggtcatgatc gtcatcgcgt
     8221 atgcgacgcg taccgtctgg acgctggtgt tcggcgcgat ggccgccgcg gccgtgcgca
     8281 cgacgctggg ccatcgactg ctgccaggct accgcgcccg cttccgggtg gacaaggacg
     8341 ccctcgacga gatcctcgcg agcggcaagt ggatcttcct gtcgtcgatc atgtccttcc
     8401 tggccatgaa cagcgacaag gtgctgctgg gcggcctcat cgacgcgcgc gcgttcggcc
     8461 tgtactcgat cgcgctgctg atggccaacg tgctgcaggg cctggcgacc aagatgtgca
     8521 tgagcatcgt ctacccggcg ctcgccgagg tgcaccgcga ccggccgcag gacctggcca
     8581 agacgatcgc gaagttccag tggggctacg acgcgctcgt gacctcgctc gcggcgctgc
     8641 tgatcacggc ggcgccggcg ctgatcggcc tgatgtacga ccaccgctac cacgacgccg
     8701 gctggatgct gtcggtgttg gcggtcgggg tgatcgggct gcgcttccag atcgtcgagg
     8761 aatgctacca ggcggtgcac aagccgcagt acgccacctt cgcgaacctg atccggctgt
     8821 gcacgctcgc cggcggcatc ttcatcgccc gccacctgtg gggcatgacg ggcgcgatcg
     8881 cggccgtcgc ggtcaccacc ttcgcggcgt ggccgctggc gatctggttc aaggtgcgcc
     8941 agcacgtctt cacctggcgc gcggaggccc tgctggtgcc cgcgatcgtc ggcggcctgg
     9001 cctgcggctg ggtgttgcgc caggtcatcg agtgggcggc gccgggacgc ttcctcggct
     9061 gaggcgggac acggtcccgt acggggccgg ccgggccgcc gcccccggtc aggccttgac
     9121 gagccggtag tagccgagcc gccgggcgag cgacttccag cgctgccccg acaggtaggt
     9181 ctcgcgcgtc agcggcacga acggctgcgg cttggcatgc gcgtccacga tgcccggcgc
     9241 cggcatgtag ccggcgtcga tgaagccggg gatccaccac ttggcggggc cgccgccgtg
     9301 gaagcacttc atcttcgaga agtcgtcgct gcgcagcacc cgcacgcccg agatccccag
     9361 cttcagcgca aggcgcttcc actgcgagtt ctgccagcag tgccagtgcg tggggtcgag
     9421 gtagccgccg ccctcgcgca gggcgacgcc cgaccagatg atggcctcga tgtgcatctt
     9481 gcgcggcaac tcgccgccgc ggcgcatcgc cgccaccatc gcgttcatcc agccgaggtc
     9541 gaggttgtgg cgcagctggg ccacgccgat gtcgacgtga tgcgcgagcg cgacgccgcc
     9601 gtcgtacgcg ttcatcacgg tctcgtgcgg cagcaggcag tgcgggcgct ggcgcatcag
     9661 caggacgccg cgcgcgggcg tgggctcgaa gcggaagcgg ttcgggaagt agaggtcggg
     9721 gtcgagcagc aggatctcct cgtccggctc ggccagcagc agcgggtcgg tgatcttgcg
     9781 ccagcacgga tggccctcgc gcaggtgctt cagctcgggg aaggcggcga agcggtcgtc
     9841 cgcggcggcg tcgagctcgg ccttcgtgtg aacggaccag gcatgacggg cctcgatgcc
     9901 gagctcctgc atcgcggcgg tgatcctggc cttgtcctcg atctcgtcgg tgatgatgcg
     9961 cacgtcgagc gcgtccatcg aatggcggca cagcgactcg agcgagcgcc gtgcgtaggg
    10021 cagggagccg gcgcccatca cgcagtaggc cttgcggcgg gcgggcgcgg tgtcttgggt
    10081 catgtgcgaa agattccgtt gggaagatgc ggcggtcgcg gattcgcggc gctcaggcgc
    10141 gcgcgacgac gtcgcggtag atgtccagga tgcggtcgac cttgcgctcc cagtcgaagc
    10201 ccgcctccac gcgcgcgcgc gccgcggcgc ccatcgcacg gcggcgctcg ggctcgctcg
    10261 cgagcgcgac catcgcgtcg gcgaagccct gcaccaggcg ctcgcgcgat tcgggcggca
    10321 ccaacacgcc gcagtgctcg tcgaggtagt cgagcggccc gccccacgcg gtcgcgatga
    10381 ccggcatgcg gcaggccatc gcctcgagca ccacggcgcc accgcactcc cacaggctcg
    10441 ggagcacgag gacgtcgctg cactgcagct cgcgcgcgca atcggcctgc gacatccagc
    10501 ccgcgaagtg caggcggccg gggacgccgg ggccgtcgtc gcgcaggccc agcgtgccgg
    10561 ccagcgcctg cagggcgggg cgctcggggc cgtcgccgat gagggtgagc gtcatcgggg
    10621 cgcggccgcg ggcgcgctcg aaggcgtgca gcagcaggtc gacggccttc cagtcgacga
    10681 ggcggcccat gtacacgaag cgcgtcgcgg ccggggcctg cgcgggatcg gacgcctggc
    10741 cggcgccgga cggcgcgccc cacaacgaca tgtcgacgcc gttctcgacc aggtggagca
    10801 cctggcccga gagtccgccc ggcagcccgc gcccggtgcg ctcgttggcg accagcaccg
    10861 cggccgccct gcgcttgccc gggaacagcg cgttcatcag gccggtgaag gcgcgcgcgc
    10921 cgcgctcgac gaggtcgacc agcgcgccct cgcggcgtcg gaaggcgggc ggaaactgca
    10981 tgttgccgtt cagcggcccg atgaccacgg gcgcgccgac gtcggcgatc agcgaaggct
    11041 cgcgcggcga gaccggcatc ggctggtgca cgacccggac gcggccctcg gcgacgagcc
    11101 ggcgcgcgat cgcccgctgc gcccgctgcg tgatcccgcg cgagacgaag ccggtcgtga
    11161 aggaggccac gcgcgccggc agcttgcggc ccaggcgcca gagcagtctc tgcgccggcg
    11221 tgtcctcgac gaacaggagg cggtcgcgat cctcggggaa gcgcgcgagc agttcgtcgc
    11281 gcacgcgcgc atgcgtgagc agccgcacgt ccagcccccg tgcacgcatc acgcggaagt
    11341 agtggaacgc gagcgccgcc tcgccgccga agcgggtgga ggcgtgctcc gacacgatca
    11401 ggatgcccat cgccttcggc gcgtcggggg cggcgtcgat cggcgcgggg atgcgcgtcg
    11461 cctcctcgtg gcgcgcgccg ccgcgcccga gccggcggtc gcgcgcctgc gtccagcgct
    11521 tgccccaggc gatgaagcgg ctctcgtagt ggtcggtcga cagctgcgcc agcgcgaagg
    11581 tcacgaggaa gaacagcggc cgcttggcgt agcgcaccag gggggtggcg ccttcgccaa
    11641 gccacgagaa catcaggcag ccgtggatga tgtagacggc gtacgagatc cgcgcgagcc
    11701 actgcagcac gcgcgccgac agcaggcgcg cggtgcgcga ctgcggcgcc gcgaacagcg
    11761 acagtcccac catcgtcgat gcgatgtagg gtcgcaggta gttcagcgcg ccggcgtcga
    11821 cgtgggcgct cgcgacgagc agcggaagca gcagccacga cagcgaaccg agccgcggcg
    11881 acggcggcgc cgcgcgcagg cggtcgaaca cgagggccag cgtgcagccg gcgaggatct
    11941 cgtcgatgcg gtactgggtg tcgatcgcca tcagctcgtg gcgcgaggcc cgcagcgcgg
    12001 tgacggccag cgcgagcagc ggcagcacgg gtagcgcgcg acgtccgaac aggcccacca
    12061 gcaacgcgat gccgaggtag aactgcacct cgacgcacag gctccagtag tgcccggtga
    12121 tcgaggtgag ccagatcggg ccggtgttgg cgatgaacag caggtgcggc aggtagtcgg
    12181 cgagcggggc gcgcgtcgcg gtcagcgcga tcagcgcggc cagccaggcg aggggcacga
    12241 tgcgcgcgag ccggcgcacg aggaagttgg ggatgtcgtc gtcgcgcagc agcacgctgg
    12301 tgatgaggaa cccggacagc gtgaagaaga tcgccatgcc gcagcccgcc acggcgccgt
    12361 tcagcagcca ggacttgggc ccgagcggca gcaggtggcc ggccagcacc agcgagatgc
    12421 tgatgccgcg ccagccgtcg agggcgttga cccggtgcga attcatgcga gcggaagaag
    12481 gcggaaaagg gctgcggggg tgcggggcgc gcgccgacgc cggcgcccac cgggcgcgcg
    12541 gggatgctag cttacctgcg tgacgccgcg gcgtgcggcg ccgcagggca agttttcggc
    12601 ctgcgggcgg ccggcgccgg cgcatgcgcc gcatccgcac ggcgagccgt caccgagccc
    12661 gggcacaatg cgtgctacga ggccgcttcg cccgggcggg gcggccatgg acggggcgag
    12721 ggcggatggg ccgcgcgaac ccgccgcagt ggcgcgaaaa gaggtgtctg gttggtctac
    12781 gcgataaaac tgctgttcgc ggtgctgctg ctgtccgcgg tgttcgcgcc gttcctgcgc
    12841 ggcgcgatgc gtggcctgat cggcgacaag gactactggc gggtctggat gatcatcgtg
    12901 gccaccgccc tggcgtcgtt cctgtcgatg cggccaccga tcttcatgtt cctggtggcg
    12961 atgatcggcc tgttcgcgcc gcgtctcctg ggcggcggca ccctggggcg gctgacggcg
    13021 tggtggctgc tgtacgtcct gtttccgccg atcagcatgt cggcggaggg cctgccgggc
    13081 atcaaccgga tcatcgagct gatggcccac cgggtcctgt cgctcgtggt gctcgtgccg
    13141 gcggtgttcg agctcctggg caacaagcag gtgcgggcca accgcatgac gcgctggacc
    13201 gacctcgcgt tcatcgccta cccgacgatg ctcatcctca cggccatccc cagcagttcg
    13261 agcaccaacg tcgcgcgttc cgtcgtcgag atggcgctcg acctgctgct gccgtactgg
    13321 gtcacgacgc gcgcgatccg cagcgccgcc gacctcaagc acctgtgcgt gcggctggtg
    13381 atcggcctcg ccttcctggc ggcggtcggc ctgatggagt cggcgctgcg caagaacgtc
    13441 tactccgagc tgcagttcat cgtcggcgtg atctggcagg cgacccaccg cctgatgcgc
    13501 gggccgttcc tgcgcgtgca ggccaccttc ccggtgccca tcgtgatggc ctacgtcatg
    13561 acgatcggct tttgcctgtg gtcctggctc ggccgcacgt cgaccaccac ccgccgcatg
    13621 cggctgctcg tgtcgttcgg cttcttcctg gcgatggccg cgaccttctc gcgcggcccg
    13681 ctgctcggat gggccagctt cgtgatcacg ctgatcgcct tgcgctggat ctcgccctac
    13741 aagtacctgg cggggctgac gctgctgttc ggcgtcgcgg tcggggcgag cgtcaccggc
    13801 accgacgaat gggcctacga ggcgctgaag accgtgttcg gttcgtcggc cgccgacacc
    13861 tcgtcgatcg actaccgccg caggctgctc gagaccgcgg tggcgctgat caagcagagc
    13921 ccgacctggg gcgtgccgaa ctacatggcc tacatgcagg acctgcggca gggcgagggc
    13981 ctcatcgaca tcgtcaacac ctatgtcgcg gtggcgctgg ccaacggcat cgtgggtctg
    14041 gtgctgttca tgtcgccgca cgtggtagtg ctggtgcggc tgctgcggcg cctgaacagc
    14101 tacgaggagc gcccgcgccg ctccgtcggg gcgttcccgg cgacgatggt ggcggcgacc
    14161 gtggcctcgc tgctgacgat cttcacgacg tccgacttct ccgccgtgcc gcggatgctg
    14221 ctgttcctga tcgccgtgcc gacggcctgg ctggcgttcg accgcgccga gcagcggcgc
    14281 gccgacgagg tcgtccccgt cggcgacgag gtggcgccgc gcggccatcc gcgcttcgcg
    14341 ccgatcccgc aggagccggg ctggcgggag ccgaactggc aatgagcgcg gacggcctgc
    14401 cggcgccggt gcacgcggcg atctccgcga gcgaccccga ctggggccgc gaggcgcgca
    14461 cgtccttctg gcagccttcc cgcgcgctgc tgcaaagcat ccgcgcctac cagcgccatg
    14521 cgggttcgcg ctcgccgatg gcggccgcgg cgcggggcct ggccgtgctg cggcaccggt
    14581 tctggagcgt ggtcaccggg gccgacatcc ccctgaactg ccggatcggc ggcgggctgc
    14641 tgctgccgca tccgaacggc gtggtcgtcc atccgtcggc gcggctcggg cccaattgcc
    14701 tgctctttca gcaggtgacc atcggcgccg gcgggcgccc gggcttcccg gtcatcggcg
    14761 gccacgtcga catcggcgcc ggcgccaagg tgctgggcgg cgtcaccgtc ggcgaccacg
    14821 cgcgcatcgg cgccaacgcc gtcgtcacgc gcgacgtgcc ggcgcacgcg acggcggtcg
    14881 gcatcccggc gcgcgtcgtc gcgaccgcag gggcggacga atgagcccgc gcgcggccgc
    14941 ctcgaagagt ccgccgaccg cagtccgaag gacggtggcc gcatgacccg tcacgacgac
    15001 ccgggcgcgt cgacgtcctc gccgcttgcg gggatgggca tcgtggtgat cggccgcaac
    15061 gagggcgagc gcctggtgcg ctgcctgcga tcggtcgcgg cggtggccgg gcgcgtggtc
    15121 tacgtggact cgggctcgac cgacggctcg gtcgccgccg cgcgcgccgc gggcgccgac
    15181 gtcgtcgagc tggacctgcg cacgccgttc accgcggcca aggcgcgcaa cgccggctgg
    15241 cagcgtttgc agcagagctt gccgcaattg tcgagcgtgc acttcctgga cggcgacgtc
    15301 gaggtcgtcc ccggctggct ggagacggcc gccgccgagc tcgcgcgtca tcccgaggcg
    15361 gccgtcgtct gcggccgccg gcgcgaacgc catccggaac gctcggtgtt caacctcctg
    15421 tgcgagatcg agtggaacgc cgccaccggt gaggtgcgcg cctgcggcgg cgacgcgctg
    15481 atgcgcctgg acgcgctgcg cgccgtcggc ggctaccgcc cgggactgat cgcgggcgag
    15541 gagcccgagc tgtgcgtgcg cctgcgcgcc gccggctgga agatccgccg cctgccgcag
    15601 gacatggcct ggcacgacgc cgcgatgacg cgctggtcgc agtggtggca gcgcagcaag
    15661 cgcgcgggcc atgcgttcgc cgagggcgcg tcgctgcac
//
LOCUS       DASVLN010000480         3372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_455386, whole genome shotgun sequence.
ACCESSION   DASVLN010000480 DASVLN010000000
VERSION     DASVLN010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455386"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VF453_11840"
     CDS             complement(<1..1079)
                     /locus_tag="VF453_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7688395.1"
                     /translation="MPSVASDPATLLPWSVALVYLLAALSTRLVHPEALARHALRAAS
                     VAVLAASLALAAALASRRPPVTSVVLLLVSVIAWVIVRFSQRHLDGEAGLATYHRSLL
                     QALAAISIVATTQHLLVLALAWIGLGLPLHRLLAFYGGRPQALRVAHAKGVFDRAGDV
                     LLLAAVGLLCAGCGSFDIGQVLRAAADATLPAAATAGAVLLALAVAMRSAQLPFHGWL
                     IQVMEAPTPVSALLHAGVVNLGGVVLLRLAPLVDAVPAARIVLLVFGIPTAALGALVA
                     STRISIKVALAWSTCAQMGFMLLEAGLGWWPALLLHLIGHSLYKAHAFLAAGGAVRQA
                     VAASLLQPSTASAGRRQAGVTAVAFA"
     gene            1176..2081
                     /locus_tag="VF453_11845"
     CDS             1176..2081
                     /locus_tag="VF453_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688396.1"
                     /translation="MSVRLNFHHLHYFWAVAKEGHLTRAAQRLHVSQSALSTQIRQLE
                     EQLGQSLFERRGRALKLTEAGQMALGYAEQIFSIGSELVAVLHHGGRPQQQVLRIGAV
                     STLSRNFQENFLRPLLPRDDVALVLQSATLSELLTRLRVHTLDLVLSNRRVQATADEP
                     WRCRRIARQPVSLVGPKRPRRRAFRFPEDLTDLPLLLPSRDSDIRSGFDLLCEQLGVR
                     YLVRAEVDDMAMLRLTARDSGSVALLPTVVVQDELRSGRLVQYAVVPNLYEDFYAITV
                     QRHFMPPLLKELLGRPEQEVLGTAS"
     gene            complement(2131..2475)
                     /locus_tag="VF453_11850"
     CDS             complement(2131..2475)
                     /locus_tag="VF453_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688397.1"
                     /translation="MRLKQALADLIVLRPTDERFADALSEFAAHVQTQQALDHQRLIP
                     VLRDVMEVDERRELCNDMELLFDAGDAPVRAESPPGRPPSELIREAEIVLSSLVRSSG
                     AADPSGSPTAAG"
     gene            complement(2649..2724)
                     /locus_tag="VF453_11855"
     tRNA            complement(2649..2724)
                     /locus_tag="VF453_11855"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2689..2691),aa:Thr,seq:cgt)
     gene            2962..>3372
                     /locus_tag="VF453_11860"
     CDS             2962..>3372
                     /locus_tag="VF453_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX7688398.1"
                     /translation="MLIFANPVHHAHEGRHEIYRGRLVPCHETPARLDYVLAELQRRP
                     LGEQRAPGPVDRALLARVHRADYLDFLENVWRDWIALDPANAGIDVLPAVWPVRGFRT
                     DVSPTNFSARVGRYSFDAGSPVTAGTWAAASAGAA"
BASE COUNT          539 a         1198 c         1152 g          483 t
ORIGIN      
        1 gcgaaggcga cggccgtcac gcccgcctgg cgccggccgg ccgaggcggt ggacggctgc
       61 agcagcgaag cggcgaccgc ctgccgcacc gcgccgcccg ccgcgaggaa ggcgtgcgcc
      121 ttgtacagcg agtggccgat caggtgcagc agcagcgccg gccaccagcc cagcccggcc
      181 tccagcagca tgaagcccat ctgcgcgcag gtcgaccaag cgagcgccac cttgatgctg
      241 atgcgcgtgc tcgcgacgag cgcccccagt gcggcggtcg ggatgccgaa gaccagcagc
      301 acgatgcgcg ccgccgggac ggcgtccacc agcggcgcca ggcgcagcaa caccaccccg
      361 ccgaggttga cgacgccggc atgcagcagc gccgagacgg gcgtcggcgc ttccatcacc
      421 tggatcaacc agccgtggaa cggcaactgc gccgaacgca tcgccaccgc cagcgcgagc
      481 agcacggcgc ccgccgtcgc cgccgccggc agcgtcgcgt cggccgcggc gcgcagcacc
      541 tggccgatgt cgaagctgcc gcatcccgcg cacagcagcc ccactgcggc gagcaggagc
      601 acgtcgcctg cccggtcgaa gacgcccttg gcgtgggcga cgcgcagggc ctgcggtcgc
      661 ccgccgtaga aggcgagcag ccggtgcagc ggcaggccga ggccgatcca ggccagcgcg
      721 agcaccagca ggtgctgggt ggtcgcgacg atcgagatcg cggcgagcgc ctgcagcagc
      781 gagcggtggt acgtcgcgag gcccgcttcg ccgtccaggt ggcgctgcga gaagcgaacg
      841 atgacccacg cgatgaccga caccagcagc agcacgaccg acgtgacggg cggccgccgc
      901 gacgcgagcg cggccgccag cgcgagcgac gccgcgagga cggcgaccga ggccgcgcgc
      961 aaggcgtgcc gtgccagggc ctcgggatgg accaggcgtg tcgacagggc ggccaacagg
     1021 tagacgagcg cgacgctcca cggcaggagc gtcgcgggat cggaggcgac ggagggcatg
     1081 gcaggttcgt gaagtggcgt tgcacgtact ttgctgaacc gacgaccatc tgtaaaattc
     1141 attgttcatt gcatttcgtt cacatcaatg gaacgatgtc cgtgcgactg aacttccacc
     1201 atctccacta tttctgggcc gtggccaagg agggccacct gacgcgcgcc gcgcagcggc
     1261 tgcacgtctc gcagtccgcg ctgtcgacgc agatcaggca gctcgaggag cagctcgggc
     1321 agtcgctgtt cgagcgccga ggccgcgcgc tgaagctcac cgaagccggt cagatggcgc
     1381 tcggctacgc ggagcagatc ttctcgatcg gcagcgagct cgtcgcggtg cttcaccacg
     1441 gcggccgacc gcagcagcag gtgctgcgca tcggagcggt gtcgacgctg tcgcggaatt
     1501 tccaggaaaa cttcctgcgc ccgctgctgc cccgcgacga tgtcgcactg gtgctgcagt
     1561 cggcgacgct gtccgagctg ttgacgcgcc tgcgtgtcca caccctggac ctcgtgctgt
     1621 ccaaccgccg cgtgcaggcc acggccgatg agccatggcg ctgccgtcgc atcgcacggc
     1681 agccggtcag tctcgtcgga ccgaagcggc cgcggcgccg tgccttccgc tttcccgagg
     1741 atctcaccga cctgccgctg ctgctgccca gccgcgacag cgacatccgt tcgggcttcg
     1801 acctgctctg cgagcaactg ggcgtgcgct acctcgtacg ggccgaagtc gacgacatgg
     1861 cgatgctgcg gctgacggcg cgagactccg gcagcgtcgc gcttcttccc accgtggtcg
     1921 tgcaggacga gttgcgcagc ggccggctcg tgcaatatgc cgtcgtcccc aacctctatg
     1981 aggacttcta cgcgatcacc gtgcagcggc acttcatgcc gccgctgctg aaggagttgc
     2041 tcggacggcc cgagcaggaa gtgctgggca cggcctcgtg atcgcgcaag ggcaccgccg
     2101 tcgcgctgcc gatcgcggga cgatcgcggt ctagcctgcc gccgttggcg aacccgatgg
     2161 atcggccgcc cccgagcttc gtaccagcga cgacagcacg atctcggctt cgcggatgag
     2221 ctcgctgggc ggtcgtccgg ggggcgactc cgcgcggacc ggggcgtcac cggcgtcgaa
     2281 caggagctcc atgtcgttgc acagctcgcg gcgctcgtcg acctccatca catcgcggag
     2341 caccgggatc agcctctggt ggtcgagcgc ctgctgggtc tgcacgtggg cggcgaactc
     2401 ggacagcgcg tcggcgaagc gctcatcggt cgggcgaagc acgatcaggt cggccagcgc
     2461 ctgcttcagg cgcacgtgcg ccgtcatcgc atccagcggc gcgaagaccc ggcgccgcac
     2521 tcgaagggca tcgtctacac gcgttgcctc gcggcgccga cagcttcccc gtcggtcgga
     2581 cgaatctccc aggcatcccc caaaaacaaa cgggtcagta gctacgacgc taccaacccg
     2641 catgaatctg gtgccgatgg acggactcga actgtcgacc ttcgcattac gaatgcgctg
     2701 ctctaccaac tgagctacac cggctttttg caacgtgctc gacctttgtc ggtcgtcgac
     2761 gccgcgaagc ctatgattct agcgcaactt tcttggcgat gaaaagcgtc gtgcgaaaaa
     2821 tctttggcac tgccggccgg tgccggggcg tggccgaagg gcccgcgatt cgtacctgga
     2881 gcgttaaagt cgtcgtgtcc tgcatgctcc gccgcgccgc gcgaagcgcc cggcggcccg
     2941 tcattcacaa cgctcgccgc gatgctgatc ttcgccaacc ccgtccacca cgcgcacgaa
     3001 ggccgccacg agatctaccg cggccgcctc gtgccttgcc atgagacgcc ggcgcgtctc
     3061 gactacgtcc tcgccgaact gcaacggcgg ccgctgggcg aacagcgcgc gccggggccg
     3121 gtcgaccgag cgctgctcgc gcgagtccac cgcgcggact acctcgactt cctcgagaac
     3181 gtctggcggg actggatcgc gctcgatccg gcgaacgccg ggatcgatgt cctgcccgcc
     3241 gtgtggcccg ttcgcggctt ccgcacggac gtttcgccga ccaacttctc ggcgcgagtg
     3301 gggcgctatt ccttcgacgc cggctcgccc gtgacggcgg gcacctgggc ggcggcgtcg
     3361 gcgggggcgg cc
//
LOCUS       DASVLN010000481         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_24766, whole genome shotgun sequence.
ACCESSION   DASVLN010000481 DASVLN010000000
VERSION     DASVLN010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24766"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..938)
                     /locus_tag="VF453_11865"
     CDS             complement(165..938)
                     /locus_tag="VF453_11865"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX7688399.1"
                     /translation="MGTTTEAAVLSVRHWTDRLFSFTTTRDAGFRFASGQFVMLGLPV
                     DGRPLLRAYSIASSPYDPTLEFFSIKVPDGALTSRLQHVRPGDKVLLGTKPTGTLLLQ
                     NLLPGRTLWLLATGTGLAPFLSIVRDLDAYTRYERVVLVHGVRRVQDLAYADLLRTEL
                     PRHEYLGEAVRSQLVYCPLVTREPFARQGRIPDLIASGRLESGLDLPPIDAARDRCMI
                     CGSQAMLADTRAVLEARGLREGNLSAPGTYVIERAFVER"
     gene            1114..2211
                     /locus_tag="VF453_11870"
     CDS             1114..2211
                     /locus_tag="VF453_11870"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX7688400.1"
                     /translation="MNRTASVPAPAAPPAGFPHRREIRGWIAPLAEPSTVRALALVLL
                     DLALYVAGLAAVVLLRSAWLKLAAGLATGLVIARLFILGHDACHQALTRHRGLNRWIG
                     RLVFLPSLTPYSLWEVGHNVVHHGYTNLRGMDFVWEPRTLAEYRALPPWRRALERVYR
                     SGWAPWLYYLVEIWWARLLLPDRRRMPSRRPEFRRDGWLACGFAALWIAGLGLAAAAT
                     RQSVAGLVLLGFVVPFVAWNALIGFVVYVHHTHTGVAWYQDKASWAAAMPFVSTTVHL
                     TFRTPAGALLHHITEHTAHHVDMGVPLYRLKAAQRVLEARLRRHIVVQPFSWRWYAET
                     ARCCKLYDFEAGRWTDFEGRPTTPAPAGGFA"
     gene            2208..>2689
                     /locus_tag="VF453_11875"
     CDS             2208..>2689
                     /locus_tag="VF453_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688401.1"
                     /translation="MTADLQSTAPPRRPTATHRTTPRGRPDLRRRLDELCEAARQLDA
                     LVDRLDRADALARASSAAILERFDLLLADIRTQEERALLPDLSREGDSSLLRTVDRLH
                     VDLGWLGANWHELRPQIDGVACGQSWVDPDALRDGAGLFAALVREHVALAGAAAERNA
                     G"
BASE COUNT          325 a         1036 c          964 g          364 t
ORIGIN      
        1 ccttgtggac ggcgtggcgc cggatcgcgg gacgcggcca ggccgaggtg cgggcgctcg
       61 gctaccgcgt cgccggcgcg gcgctgtcgg tcaccgcggc gatcgcgctg gcgcatgccc
      121 tgcacgagcg catcgcggcg ctctgccaag cctgagcgtc gcggctatcg ctccacgaag
      181 gcgcgctcga tgacgtaggt gccgggcgcg gacaggttgc cttcccgcag cccccgcgcc
      241 tcgagcaccg cccgcgtgtc cgccagcatc gcctggctgc cgcagatcat gcagcggtcc
      301 cgggcggcat cgatgggggg caggtcgagc ccggattcca ggcggccgct cgcgatcagg
      361 tccggaatgc ggccctggcg cgcgaacggc tcgcgcgtga ccagcgggca gtacaccagc
      421 tgcgagcgca ccgcctcgcc gaggtactcg tgccgcggca gctccgtgcg cagcaggtcg
      481 gcgtaggcga gatcctgcac ccgccggacg ccgtgcacga gcaccacccg ctcgtagcgc
      541 gtgtatgcgt cgaggtcgcg cacgatgctg aggaaggggg cgaggccggt gcccgtggcc
      601 agcagccaca gcgtgcgccc gggcagcagg ttctgcagca gcagcgtgcc ggtcggcttg
      661 gtgccgagca gcaccttgtc gccgggacgg acgtgctgca ggcgcgaggt cagcgcgccg
      721 tcgggcacct tgatgctgaa gaactccagc gtcgggtcgt acggcgagct cgcgatgctg
      781 taggcgcgca gcaggggccg cccgtcgacg ggcaggccga gcatcacgaa ctggccgctc
      841 gcgaagcgga atccggcatc gcgcgtggtc gtgaaggaga acaggcggtc ggtccagtgg
      901 cggacggaca ggacggcggc ttcggtggtg gttcccatcg cgtggaggct cctcgttgga
      961 cgccagcatc cccgcgggcg ccgcgcgcgc ccttgccctg gcgcaaggcg gacgcgcgcg
     1021 cggccggcgt cgagcgccgg acttgacgca cgacaaaccg cggcgggccg ccggggcctg
     1081 aaatcgctgg cggaaccgat ctcacccacc acgatgaacc gcaccgcctc cgttcccgcg
     1141 ccggccgccc cgccggccgg cttcccgcat cgccgcgaga tccgcggctg gatcgccccg
     1201 ctcgccgagc ccagcaccgt gcgcgcgctc gcgctggtcc tcctcgacct cgcgctctac
     1261 gtcgccggcc tcgcggccgt cgtgctgctg cgcagcgcgt ggctgaagct cgccgccggc
     1321 ctcgcgaccg ggctggtcat cgcgcggctg ttcatcctcg gccacgacgc ctgccaccag
     1381 gcgctcacgc gccaccgcgg cctcaaccgc tggatcgggc ggctcgtgtt cctgccctcg
     1441 ctcacgccct acagcctgtg ggaggtcggc cacaacgtcg tccaccacgg ctacacgaac
     1501 ctgcgcggca tggacttcgt ctgggagccg cgcaccctcg ccgagtaccg cgcgctgccg
     1561 ccgtggcggc gcgcgctcga gcgcgtctac cgcagcggct gggcgccctg gctgtactac
     1621 ctcgtcgaga tctggtgggc acggctgctg cttcccgatc gtcgccggat gcccagccgc
     1681 cgccccgagt tccggcgcga cggctggctc gcgtgcggct tcgccgcgct gtggatcgcg
     1741 ggactcgggc tcgcggcggc ggcgacgcgg cagtcggtcg ccggcctggt gctgctcggc
     1801 ttcgtcgtgc ccttcgtcgc ctggaacgcg ctgatcggct tcgtcgtcta cgtccaccac
     1861 acgcacaccg gcgtggcgtg gtaccaggac aaggcgtcct gggccgcggc gatgcccttc
     1921 gtgtcgacca ccgtgcacct caccttccgc acgccggcgg gcgcgctgct gcaccacatc
     1981 accgagcaca cggcccacca cgtcgacatg ggcgtcccgc tgtaccggct gaaggccgcg
     2041 cagcgcgtgc tcgaggcgcg gctgcgccgg cacatcgtcg tgcagccgtt ctcgtggcgc
     2101 tggtacgccg agaccgcgcg ctgctgcaag ctctacgact tcgaggccgg tcgctggacg
     2161 gacttcgagg gccggcccac cacgccagcg cccgccggag gcttcgcatg accgccgatc
     2221 tccagtccac cgccccgccc cgccgcccga cggccacgca ccggacgacg ccccgcggcc
     2281 ggcccgacct gcgacgtcgc ctggacgagc tctgcgaggc ggcgcgccag ctcgacgcgc
     2341 tcgtcgaccg gctcgaccgc gccgacgcgc tcgcgcgcgc gtcctccgcc gcgatcctgg
     2401 agcgcttcga cctgctgctg gccgacatcc ggacgcagga ggagcgcgcg ctgctgcccg
     2461 acctctcgcg ggagggcgac tcctcgctgc tgcgcaccgt cgaccgcctg cacgtcgacc
     2521 tcggctggct cggcgcgaac tggcacgaac tgcggccgca gatcgacggc gtcgcctgcg
     2581 ggcagtcctg ggtcgacccg gacgcgctgc gcgacggcgc cgggctcttc gccgcgctgg
     2641 tgcgcgagca cgtcgcgctg gccggcgccg ccgccgagcg caatgcggg
//
LOCUS       DASVLN010000482         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_24817, whole genome shotgun sequence.
ACCESSION   DASVLN010000482 DASVLN010000000
VERSION     DASVLN010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24817"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VF453_11880"
     CDS             complement(<1..690)
                     /locus_tag="VF453_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7688402.1"
                     /translation="MLRHIAAFEWRYQLRNPVLWVGALLFLVSGFGATVSDYVQIGSA
                     GSFHKNSPYALLQATGVMGLFVTFVTVALVAGAVLRDDETGFAALLHATPLRKFDLLV
                     GRFAGAVAAALVVVAATPLGILLGTAMPGLDAERFGPTLPGDYLYAIFLFGLPTLLVT
                     AAAYFAVAAATRSMVWSYAVAIALLAAYFVAVGLVHADPAWHRAAAFLDPFGLISLSY
                     VTRYWTTTELDT"
     gene            complement(701..1612)
                     /locus_tag="VF453_11885"
     CDS             complement(701..1612)
                     /locus_tag="VF453_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7688403.1"
                     /translation="MLSISSLTHVYPNGTRALDDVSLEIPPGLYGLLGPNGAGKTTLM
                     RCIATLQRPTSGAIRFGDIDVLAEPARLRGTLGYLPQEFGVYPRVSVYAMLDHLAVLK
                     GLVDAAARRETVETLLRQVNLWEHRKRAIAGLSGGMRQRFGIAQALIGNPSLMIVDEP
                     TAGLDPEERNRFNQLLNEIGATKVVLLSTHIVDDVAELCSRMAILVGGRIVSAGSPQA
                     LIDGLQGRVWTRVVEPGELPALRKQFELIATRLRAGRTVIHVVADSAPDPRFQPVHAG
                     LADVYFSTLHEQRRRLGRQGPADADAA"
BASE COUNT          281 a          640 c          734 g          226 t
ORIGIN      
        1 cgtgtcgagc tcggtggtcg tccagtaccg ggtgacgtag gacaggctga tcaggccgaa
       61 cggatcgagg aaggccgcgg cgcggtgcca ggccgggtcc gcgtgcacga ggccgacggc
      121 gacgaagtag gccgcgagca gcgcgatcgc gaccgcgtag ctccagacca tggagcgcgt
      181 cgccgccgcg accgcgaagt acgcggccgc cgtcacgagc agcgtcggca gcccgaacag
      241 gaagatcgcg tagaggtagt cgccgggcag cgtcgggccg aagcgctcgg catccaggcc
      301 cggcatggcg gtgccgagca ggatgcccag cggcgtcgcc gcgacgacca cgagcgccgc
      361 cgcgaccgcc ccggcgaagc ggccgacgag caggtcgaac ttgcgcagcg gcgtcgcgtg
      421 caggagcgcg gcgaagccgg tctcgtcgtc gcgcagcacc gcgccggcga ccagcgccac
      481 ggtcacgaag gtcacgaaca ggcccatcac gcccgtcgcc tgcagcagcg cgtagggcga
      541 gttcttgtgg aagctgccgg cgctgccgat ctggacgtag tcgctgaccg tcgcgccgaa
      601 gcccgagacg aggaacagca gcgcgccgac ccagagcacg ggattgcgca gctggtagcg
      661 ccattcgaag gcggcgatgt ggcgcagcat cgggcggccc tcaggcggcg tcggcgtcgg
      721 cgggcccctg ccggccgagg cggcggcgct gctcgtgcag cgtcgagaag tagacgtcgg
      781 ccaggccggc gtgcacgggc tggaagcgcg ggtcgggcgc cgagtccgcg accacgtgga
      841 tcaccgtgcg gcccgctcgc agccgggtcg cgatcagctc gaactgcttg cgcagggccg
      901 gcagctcgcc gggctcgacg acgcgcgtcc agacgcgccc ctgcagcccg tcgatcagcg
      961 cctgcggcga gcccgcgctg acgatgcggc cgccgacgag gatcgccatg cgcgagcaca
     1021 gctcggcgac gtcgtccacg atgtgggtcg acagcagcac gaccttggtc gcgccgatct
     1081 cgttgagcag ctggttgaag cggttgcgtt cctcggggtc gaggcccgcg gtcggttcgt
     1141 cgacgatcat cagcgacggg ttgccgatca gcgcctgcgc gatgccgaag cgctggcgca
     1201 tgccgcccga cagcccggcg atcgccctct tgcggtgttc ccagaggttg acctggcgca
     1261 ggagcgtctc gaccgtctcg cgccgggctg ccgcgtcgac caggcccttg agcacggcga
     1321 ggtggtccag catcgcgtag accgacacgc gcgggtagac gccgaactcc tgcggcaggt
     1381 agccgagcgt gccgcgcagg cgcgcgggct cggccagcac gtcgatgtcg ccgaagcgga
     1441 tcgcgccgct ggtcgggcgc tgcagcgtgg cgatgcagcg catgagcgtc gtcttgccgg
     1501 cgccgttggg ccccagcagg ccgtacaggc cgggcgggat ctcgagcgag acgtcgtcga
     1561 gggcacgcgt gccgttcggg tagacgtggg tgagcgacga gatcgacaac atgctgcggg
     1621 cgtgggcgtg ggcgcggagg aaaggcgggg attgttccag ggctcgcact ctaccggctg
     1681 tccggcgaag accccacggc ggtgcgacgg accgacaaat gcttgggaca ggccgggcgc
     1741 cgcagggatg gccgccgggg ccgtcgcctg cgcgacgccg gcggggtcga gcgacgggca
     1801 agaagccggc ccggggcgcc ctcgaggctt cagggcgccg tggcccccag caggcgcgcc
     1861 atcgcggcgt cgccgccgtc g
//
LOCUS       DASVLN010000483         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_400239, whole genome shotgun sequence.
ACCESSION   DASVLN010000483 DASVLN010000000
VERSION     DASVLN010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400239"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF453_11890"
     CDS             complement(<1..231)
                     /locus_tag="VF453_11890"
                     /inference="COORDINATES: protein motif:HMM:NF023299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HEX7688404.1"
                     /translation="MSTPITADALRIGMFVHLNVGWMSHPFPLSSFKLTSAEQIETIR
                     ALGLSRLTWSPERSDPEAAAEPASTGAGADAPE"
     gene            448..1650
                     /gene="pcaF"
                     /locus_tag="VF453_11895"
     CDS             448..1650
                     /gene="pcaF"
                     /locus_tag="VF453_11895"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX7688405.1"
                     /translation="MTEAFICDALRTPFGRYAGALSAVRADDLGAIPIRALLARHPAL
                     DPAAIDDVVYGCANQAGEDNRNVARMSALLAGIPVDVPGVTLNRLCGSGLNAVGSAAQ
                     AIRAGEAGLLVAGGVESMSRAPFVMPKAGAAFARENAVYDTTIGWRFVNPLMKAQYGI
                     DSMPETAENVAGEFGVARADQDAMALRSQQRAVAAQARGFFDAEITPVTVPQKKGEAL
                     VVARDEHPRDTSLEALAKLKPIVRPDGTITAGNASGVNDGACALLLASEAAARTHGLV
                     PRARVLGMAVAGVAPRIMGIGPAPASRKLLDRLGLAIAQMDVIELNEAFAAQGLAVLR
                     QLGVADDDPRVNPNGGAIALGHPLGASGARLVTTAVNQLHATGGRYALCTMCIGVGQG
                     IALVVERV"
     gene            complement(1744..2142)
                     /locus_tag="VF453_11900"
     CDS             complement(1744..2142)
                     /locus_tag="VF453_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7688406.1"
                     /translation="MQRVTGIGGIFFKARDVDALRAWYRRHLGLDVQEWGGVALAPAP
                     TQAPGSRTPLASHTTWCIFPADSDYFGGGTRSAPFMINYRVADLDALLAALRREGCKV
                     DDKTEVSEYGKFGWVTDPEGNRVELWQPPA"
     gene            complement(2174..2614)
                     /locus_tag="VF453_11905"
     CDS             complement(2174..2614)
                     /locus_tag="VF453_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7688407.1"
                     /translation="MNAPTAADDGEPVRYVGFWVRVAASLIDTVLALFVIVPIMVFLF
                     GNGWAYAEGAAAVIINWVLPGAAFVAFWVARGATPGKMAFSAVVVDAATHETPDFWQA
                     LTRYVGYFVSTTPLFAGLVWVAFDARKQGWHDKMARTVVIYRRR"
BASE COUNT          406 a          989 c          946 g          371 t
ORIGIN      
        1 ctccggcgcg tccgccccgg cgccggtgga cgcgggctcg gcggcggcct cgggatcgct
       61 gcgctcggga ctccaggtca ggcgcgacaa gcccagcgcg cggatcgtct cgatctgctc
      121 ggcggacgtc agcttgaagc tggacaacgg gaacgggtgc gacatccacc cgacgttcag
      181 gtggacgaac atcccgatgc gcaatgcgtc cgcggtgatt ggagtcgaca ttcgaagttt
      241 tccttggccg ccccggccag ggtcggggcg ttttggctct cacgtgggaa tcggccgtac
      301 acacccctga cttgagcgga cagagtccac caaaagccgg gcggccaccc ggagcgggca
      361 aaatagcccc tcattcatca cgtttgcgaa taaaagttct tctagcgaac tttccgcaac
      421 attcggaaac catttcgaga cgctcccatg accgaagcct tcatctgcga cgccctgaga
      481 accccgttcg gccgctacgc gggcgcgctg tccgccgtcc gggccgacga cctgggagcg
      541 attccgatcc gcgccctgct cgcccgccat ccggcgctcg accccgccgc gatcgacgac
      601 gtcgtctacg gctgcgccaa ccaggccggg gaggacaacc gcaacgtcgc gcgcatgagc
      661 gccctgctcg ccggcatacc cgtggacgta cccggcgtga cgctgaaccg gctgtgcggc
      721 tcgggcctga acgcggtcgg cagcgccgcc caggcgatcc gcgcgggcga ggccggcctg
      781 ctggtggcgg gcggcgtcga gagcatgagc cgcgccccct tcgtcatgcc caaggcgggc
      841 gccgccttcg cgcgcgagaa cgcggtctac gacaccacca tcggctggcg cttcgtcaac
      901 ccgctgatga aggcgcagta cggcatcgac tcgatgcccg agaccgccga gaacgtcgcc
      961 ggggaattcg gcgtcgcgcg cgccgaccag gacgcgatgg cgctgcgctc gcagcagcgc
     1021 gcggtcgcgg cgcaggcgcg cggcttcttc gacgccgaga tcacgccggt cacggtgccg
     1081 cagaagaagg gcgaggcgct ggtcgtcgcg cgcgacgagc atccgcgcga cacctcgctg
     1141 gaggcgctcg ccaagctcaa gcccatcgtg cgcccggacg gcacgatcac cgctggcaac
     1201 gcctcgggcg tcaacgacgg cgcctgcgcc ctgctgctgg ccagcgaggc cgccgcgcgc
     1261 acccacggcc tggtgccgcg cgcgcgggtg ctgggcatgg ccgtggccgg cgtggcgccg
     1321 cgcatcatgg ggatcgggcc ggcgccggca tcgcgcaagc tgctcgatcg cctcggcctg
     1381 gcgatcgcgc agatggacgt catcgagctg aacgaggcct tcgccgcgca gggcctggcc
     1441 gtcctgcgcc agctgggcgt cgccgacgac gatccgcgcg tgaaccccaa cggcggcgcc
     1501 atcgcgctgg gccacccgct gggcgccagc ggcgcgcgcc tggtgacgac cgccgtcaac
     1561 cagctgcacg cgaccggcgg ccgatacgcc ctgtgcacga tgtgcatcgg cgtcggccag
     1621 ggcatcgcgc tggtcgtcga acgcgtctga gaacggttct ggtcgcacgg atcgcctcca
     1681 tacgggacga ggtatcgcac gatacccggt tccagtatgc tcggcgagag ggtcggctgc
     1741 ctgtcaggcc ggcggctgcc agagctcgac ccggttgccc tccgggtcgg tgacccagcc
     1801 gaacttgccg tattccgaga cttcggtctt gtcgtcgacc ttgcagccct cgcgccgcag
     1861 cgcggccagc agggcgtcca ggtcggcgac gcggtagttg atcatgaagg gcgcgctgcg
     1921 cgtgccgccg ccgaagtagt cgctgtcggc agggaagatg caccaggtcg tgtgcgaggc
     1981 cagcggcgtg cggctgccgg gcgcctgcgt cggtgccggg gcgagcgcca cgccgcccca
     2041 ttcctgcacg tccagcccga ggtggcgccg gtaccacgcc cgcagcgcgt cgacgtcgcg
     2101 cgccttgaag aagatgccgc cgatcccggt cactcgttgc atcgcgtgtc tcccgtcggc
     2161 gcgcgcggcg ccttcagcgc cggcggtaga tcaccaccgt gcgcgccatc ttgtcgtgcc
     2221 agccctgctt gcgcgcatcg aaggcgaccc agaccagccc ggcgaacagc ggcgtggtcg
     2281 acacgaaata gcccacgtag cgcgtgagcg cctgccagaa atccggcgtc tcgtgcgtcg
     2341 cggcgtccac cacgaccgcc gagaacgcca tcttgcccgg cgtcgcgccg cgcgcgaccc
     2401 agaacgcgac gaaggccgcg cccggcagca cccagttgat gatcacggcg gcggcgccct
     2461 cggcatacgc ccagccgttg ccgaacagga agaccatgat cggcacgatc acgaaaagcg
     2521 ccaggacggt gtcgatcagc gacgccgcca cgcgcaccca gaagccgacg tagcgcaccg
     2581 gctcgccgtc gtccgcggcg gtcggggcgt tcacggctgc ggcctcccgg cggcgcggcg
     2641 cgcgcgcacg gcccgggcga ggcttgccag cagcggctcg gtgtcggccc agccgatgca
     2701 ggcgtcggtg at
//
LOCUS       DASVLN010000484        12240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_346589, whole genome shotgun sequence.
ACCESSION   DASVLN010000484 DASVLN010000000
VERSION     DASVLN010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346589"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1385)
                     /locus_tag="VF453_11910"
     CDS             complement(24..1385)
                     /locus_tag="VF453_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7688408.1"
                     /translation="MNEHAPEHPGAADDDHDGDATPNQEHPYEVVREMPVARLVQALR
                     RALRPAAACVVLGAAGWVLWKNPPVRTVAPGEVGVRTNRLTGGTSEFRAGSVLVVPGL
                     QALHAYPLRDRVFRPALGATADGAGAFQSSEGLSIGVDLAVRYALDPARVAAAARTLP
                     DDIDGQVVAPEVQGIAYRTLTRYSVREIFSTKRGEIQQAIESELRTRLAADGLVLRSV
                     QIGRIDLPADYRRGMEALLAQELESEKMRYTLELKDKQVKESELEAEAEKVKREKAAE
                     AAGDEQVIAAKAQEEAMKHVLPFKEKQIQQRQLEAEADKLSRIKTAEGNAEARRIEAA
                     GEADSRTRLADAEVYRLEKVGRTTSEQMARDGALLSRHPLLIQKTMADKLSDKVQVII
                     APTPADGGFIGAALLGTGRNAGTGSGTAQAVKTAATQDAADPNGADAADADADASQQG
                     GRQ"
     gene            1598..2314
                     /locus_tag="VF453_11915"
     CDS             1598..2314
                     /locus_tag="VF453_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7688409.1"
                     /translation="MANTVARVAIIEDDPPTSDQLKGWILCARPGLQVDQWFTRDDAE
                     NAIAREAYDLVVMDIELGRERHAGVALINAINKRQAGTPVLVVSAMPATIYRSIMKAL
                     DAWDYLQKTTFEEADFIDTVLEMLRAARERAPVRAATTPAAPDAELSLDPLRQGAVLW
                     RGHRVNLPLTAQRILAALHARRGEVVSYDELFQVVKTGRNRDNVRKHISTIREAFRDI
                     DPQFDGIESVPMRGFRWLAQ"
     gene            2322..4565
                     /locus_tag="VF453_11920"
     CDS             2322..4565
                     /locus_tag="VF453_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7688410.1"
                     /translation="MNRPSLRRRLARWKWPGLAFFRLRLFFRGVFLLLALATVAIALS
                     VLQDEKQLSHRAYREGFAKTMGQVAAQLRHPAGQLALLNPPPPAGATPKAGADAGVHP
                     LVLPFAALDFDDHNKVEQAVEMSGCTRQYPGGASLCVAVGSNPWAGAFVYVVGHAASR
                     APVAHAHGERDLSTASRLRVEVDLRGRHYAWIAPFEDGVSIGVEGLRGRLTGFAVDES
                     GTPATRPARDFRGWLWHGGPCLDDGADTAPRIDSHTPTRTADDCRRRTFFSVRLPVEV
                     LQDDLLRKARPVWPPADLNRIDVRVRWLAPGDGAPVFDSASPGSVPPFSLADLGAQLL
                     PGETLTLRKLGDGHVPPETIATLSGQHAPAPPLSAWMPQALNDALGYAVRGLLRRLPI
                     EDEDVPVAATETIRTAAGSYELRGVGDERSANRNLAAVATRVSWFVVAMLLALALAWG
                     AIEWTIIRRITMLTKRSRAVTAALAGDEAGPGRWTALDVADLRGPDELGVLAANLAEL
                     MQRVDESMQRERLRAEREKDQWMAVGHEIMSPLQSLIALHGGPDDPSRRYILRMQQAI
                     RVLYGSASPSEAFQSTQLQLATLELGAFLEAVAANAPHAGIADVRYEGPSHPVVVRAD
                     EYPLEDVVTHVLQNAQRHRVPGTPISIRLRADDGSAVVELHNAGPHIPPELVDRIFEY
                     GVSGDTASGIDDGAGAGEDRDRTAPQRGQGLFVAKTYMAKMGGTIAAENVADGVRIVL
                     SFPCVVA"
     gene            4686..5633
                     /locus_tag="VF453_11925"
     CDS             4686..5633
                     /locus_tag="VF453_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7688411.1"
                     /translation="MTSKSPSATIVRLARLALPALVLLALLAAGTSAQAQRLRLREAL
                     AMDDGTDDDARAEAPAGTRVLRDLAYGPDAAQRLDVYLPNQAPAPGSAPMPVILFVHG
                     GGWARGDKAMHRVVDPKVGHWVGRGWAVVSIDYRMLPTPVATQEDDVAAALAFAQAQA
                     PQWGADPKRFVLMGHSAGAHLVALLAAGARTPSSPHLQPWRGAVFLDSAALDLPFLMT
                     HRHLRLYDRAFGSDASTWTALSPLAVLQAPAAPVLAVCSTRRTESCMDADRFAAKARS
                     LGGQARVLREDLSHGEINATLGAPSDYTGQVDAFLDSLR"
     gene            5725..5955
                     /locus_tag="VF453_11930"
     CDS             5725..5955
                     /locus_tag="VF453_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013516940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7688412.1"
                     /translation="MPSAERRRPPPRLPLIDPARCTGCGRCVAACGPRVLSLETVRWR
                     KTATLHDAAGCTGCSLCERRCPFGAIAMGAAP"
     gene            6333..6662
                     /locus_tag="VF453_11935"
     CDS             6333..6662
                     /locus_tag="VF453_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688413.1"
                     /translation="MDVLAMLLVCAAIAFPMASVLIAARLWRAKLARPARFKAVGLTL
                     LYLFTGYLLTRPVPTHAVAIEPLDFTHPVENINGSVAVRSALLLLVEVAASSIAVVLL
                     LRRIMSR"
     gene            complement(7025..8776)
                     /locus_tag="VF453_11940"
     CDS             complement(7025..8776)
                     /locus_tag="VF453_11940"
                     /inference="COORDINATES: protein motif:HMM:NF038237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Ec67 family RNA-directed DNA
                     polymerase/endonuclease"
                     /protein_id="HEX7688414.1"
                     /translation="MSALADLKACVSFHDVARMLGFTPRAVAYLLYRLPEHAKYTTFS
                     VPKRSGGMRTIAAPCAQLKLLQRNLAGVLQDSLEEIQFRRGKSDGSKRPDRISHGFRR
                     RRSIVTNALQHRAQRFVFNVDVEDFFGSINFGRVRGFFIKDRDFALHPPVATVLAQIA
                     CFQNRLPQGSPCSPVISNLVGHVVDIHLTSLAKAEGITYSRYADDLTFSTNKCAFPPS
                     VAVADETVAHKWVPGGALKRLIDRGGFRLNEAKTRMQYHDSRQLVTGLVVNRKINAPD
                     EYRRHVRMLVHTYTSTGSFFFKRKGDASVGPVQEVKEVGKPEQLRGMLEYIVYASRRG
                     TVADYSAPLNSPERLYRTFVLFHDFYAPPMPLVLCEGKTDLVYIAQAARSLATSYPQL
                     ANVAADGKIKLLFRRHRYPIKGRGRLTARVLGIHGGAPDIKEFIQVLHREYEAIKAPP
                     GEQPVIITLDDDDGVRKIRAYVKHMFGKDLDQSVGFHHLFGNVYVVLTPTPFGKTSSN
                     MEDLFDAATQAVVLNGRTFDASKDNDSKTTYGKATFAYEVVAKQDAAINFMGFRPLLD
                     RFVEVLNDYRGRRATAP"
     gene            complement(9507..11306)
                     /gene="aspS"
                     /locus_tag="VF453_11945"
     CDS             complement(9507..11306)
                     /gene="aspS"
                     /locus_tag="VF453_11945"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX7688415.1"
                     /translation="MRTTYCGLVSEALIGQTVTLMGWAHRRRDHGGVIFIELRDREGH
                     VQVVCDPDRPEMFQTAEGVRNEFCLKVVGLVRARPGDKENANIASGKVEVLCHELEVL
                     NPSVTPPFQLDDENLSETTRLTHRVLDLRRPQMQKNLMLRYRVAMEVRKFLDAQGFVD
                     IETPMLGKSTPEGARDYLVPSRVHDGHFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDIETSFLTEEEIRAMFEGMIRTVFKNAIGVDLGVYPVMTYAEA
                     MRLYGSDKPDLRVKLEFTELTDVMGDVDFKVFSSAAQMAGGRVVALRVPKGAEISRGE
                     IDAYTEVVKIYGAKGLAWIKVNDVSKGREGLQSPIVKNLHDAAIAEILGRTGAVDGDL
                     IFFGADKAKIVNDAMGALRLKVGHSEFGKANGLFEDKWAPLWVVDFPMFERDDEENRW
                     NAVHHPFTAPKDGHEDLMDSDPGACIAKAYDMVLNGWELGGGSVRIHRADVQSKVFSA
                     LNISREDAQVKFGFLLDALQYGAPPHGGLAFGLDRIVTMMTGAESIRDVIAFPKTQRA
                     QDLLTGAPSMVDEKQLRELHIRLRNAVQATPAA"
     gene            complement(11451..12113)
                     /locus_tag="VF453_11950"
     CDS             complement(11451..12113)
                     /locus_tag="VF453_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX7688416.1"
                     /translation="MRKYLFAGLLVWLPLAITVGVLMWAVGLMDGVFHGILATAQHVL
                     PDRADNFIALLGGVPGLGVVLLLLLLLLTGMFVTNMVGAWWLAQWNRLLSHIPIVKSI
                     YVSVKQVSDTLFSTNGNAFREAVLVQYPHPGSWTIAFVTGRPSGEVAAALPAEHVSLY
                     VPTTPNPTSGFFLIMPRAACKPLSMSVDEALKYIISMGVVAPPAPNLLVVPEGADPNR
                     VG"
BASE COUNT         1884 a         4165 c         4213 g         1978 t
ORIGIN      
        1 ggcgtcgatg aagcgcaccg cggtcattgc cggcctccct gctgcgaggc gtccgcatcc
       61 gcatcggcgg cgtccgcgcc gttcgggtcg gcggcgtcct gcgtcgcggc cgtcttcacc
      121 gcttgtgccg taccgctacc cgtaccggca ttgcgaccgg tgcccagcag cgccgcgccg
      181 atgaagccgc cgtccgccgg agtcggcgcg atgatgacct ggaccttgtc ggacagcttg
      241 tcggccatgg tcttctgaat cagcagcgga tggcgcgaca gcagcgcccc gtcgcgggcc
      301 atctgctcgc tggtcgtcct gccgaccttc tcgagccggt agacctccgc gtccgcgagc
      361 ctcgtgcgcg agtcggcttc gcccgcggcc tcgatgcggc gcgcctcggc gttgccttcc
      421 gccgtcttga tgcgcgagag cttgtcggcc tcggcctcga gctggcgctg ctggatctgc
      481 ttctccttga acggcagcac gtgcttcatc gcctcctcct gggccttggc ggcgatcacc
      541 tgctcgtcgc ctgcggcctc ggcggccttc tcgcgcttga ccttctcggc ctccgcctcc
      601 agctcggact ccttgacctg cttgtccttc agctccagcg tgtagcgcat cttctcgctc
      661 tcgagttcct gcgcaagcag cgcctccatg ccgcggcggt agtcggcggg caggtcgatg
      721 cggccgatct gcacggagcg caggacgagg ccgtcggcgg ccaggcgggt gcgcagctcg
      781 gactcgatcg cctgctggat ctcgccgcgc ttcgtcgaga agatctcgcg caccgagtag
      841 cgcgtgagcg tgcggtaggc gatgccctgc acctcgggcg cgacgacctg gccgtcgatg
      901 tcatcgggca gcgtgcgcgc ggccgcggcg acgcgcgcgg ggtcgagggc atagcgcacc
      961 gcgaggtcga cgccgatcga cagcccttcc gacgactgga aggcgccagc gccgtccgcg
     1021 gtcgcgccca acgcagggcg gaagacgcga tcgcgcagcg gataggcatg cagcgcctgc
     1081 aggcccggca cgacgagaac gctgccggcg cggaactcgc tggtcccgcc ggtcaggcgg
     1141 ttggtgcgca cgccgacctc gcccggcgcc acggtgcgca ccggcgggtt cttccacagc
     1201 acccagccgg cggcaccgag gaccacgcag gccgcggccg gccgcagcgc gcggcgcagc
     1261 gcctgcacga gccgcgcgac gggcatctcg cggacgacct cgtacggatg ctcctggttg
     1321 ggcgtcgcgt cgccgtcgtg gtcgtcgtcg gcggcgccgg ggtgctcggg ggcgtgttcg
     1381 ttcatgtcgg ggctcctcgt tggtgtggga gccattcttc ggagcggctg ccgagcgttc
     1441 aacgacgccc caggggcgat gcgctcacgg ccgcggcggc cgtcttccgc gcgacggaag
     1501 aaacactcac gtcccgccgc gcggcggcgc cggataatct ggccgcaccg acccatcccg
     1561 gcacgcatgc gccggcgagc cgaagaagga cccgaccgtg gccaacaccg tcgcccgcgt
     1621 ggcgatcatc gaagacgacc cgccgacgag cgaccagctc aagggctgga tcctctgcgc
     1681 acgcccgggc ctgcaggtcg accagtggtt cacgcgcgac gacgccgaga acgcgatcgc
     1741 gcgcgaggcc tacgacctcg tcgtgatgga catcgaactc gggcgcgagc gccacgccgg
     1801 cgtcgcgctg atcaacgcga tcaacaagcg ccaggccggc acgccggtgc tggtggtctc
     1861 ggcgatgccg gcgaccatct accgcagcat catgaaggcg ctcgacgcct gggactacct
     1921 gcagaagacg accttcgagg aggccgactt catcgacacc gtgctcgaga tgctgcgcgc
     1981 cgcccgggag cgcgcgcccg tgcgcgccgc gacgacgccg gccgcgcccg acgccgagct
     2041 gagcctcgac ccgctgcgcc agggcgccgt gctgtggcgc ggccaccgcg tcaacctgcc
     2101 gctgacggcg cagcgcatcc tcgccgcact gcacgcgcgc cggggcgagg tcgtgtcgta
     2161 cgacgagctg ttccaggtcg tgaagaccgg ccgcaaccgc gacaacgtgc gcaagcacat
     2221 cagcaccatc cgcgaggcgt tccgcgacat cgatccgcag ttcgacggca tcgagagcgt
     2281 gccgatgcgc ggcttcaggt ggcttgccca gtaggcgcgg cgtgaaccgc ccgtcgctgc
     2341 gccgccgcct ggcgcgctgg aagtggcccg gcctcgcgtt cttccgcctg cgcctgttct
     2401 tccgcggcgt gttcctgctg ctggcgctgg cgaccgtggc gatcgcgctg tcggtgctgc
     2461 aggacgagaa gcagctgagc cacagggcat atcgcgaggg cttcgcgaag acgatggggc
     2521 aggtcgcggc gcagctgcgg catccggcgg gccagctcgc gctgctgaat ccgccgccgc
     2581 cggcgggcgc gaccccgaag gccggggcgg acgcgggggt gcatccgctg gtgctgcctt
     2641 tcgcggcgct cgacttcgat gatcacaaca aggtcgagca ggccgtcgag atgtcgggct
     2701 gcacgcgcca atacccgggc ggggcatcgc tgtgcgtggc ggtcggaagc aacccgtggg
     2761 cgggcgcctt cgtctacgtc gtcggccatg cggcctcgcg cgcgcccgtc gcgcatgcgc
     2821 acggggagcg cgatctctcg accgcgagcc ggctgcgggt cgaggtcgac ctgcgcggcc
     2881 gccactacgc gtggatcgcg ccgttcgagg acggcgtgtc catcggcgtc gagggcctgc
     2941 gcgggcggct gacgggcttt gcggtggacg agtccggcac gcctgcgacg cggccggcgc
     3001 gagacttccg cggctggctg tggcacggcg gcccgtgcct cgatgacggc gcggatacgg
     3061 caccccgcat cgactcccat accccaacgc gtaccgcgga cgactgccgc cgacgcacct
     3121 tcttctcggt gcgcctgccg gtcgaagtgc tgcaggacga cctgctgcgc aaggcccgcc
     3181 cggtgtggcc gcccgccgac ctgaaccgca tcgacgtgcg cgtgcgctgg ctggcgcccg
     3241 gcgacggcgc gccggtcttc gacagcgcga gcccgggctc cgtgccgccc ttctcgctcg
     3301 ccgacctcgg cgcgcagctg ctgcccggcg agacgctgac cctgcgcaag ctcggcgacg
     3361 gccatgtccc gcccgagacc atcgcgacgc tctcgggcca gcacgccccc gcgccgccgc
     3421 tgtcggcctg gatgccgcag gccctgaacg acgcgctcgg gtacgcggtc cgcgggctgc
     3481 tgcggcgcct gccgatcgag gacgaggatg tgcccgtcgc cgccaccgag accatccgca
     3541 ccgcggccgg cagctacgag ctgcgcggcg tcggcgacga gcgcagcgcc aaccgcaacc
     3601 tggcggcggt ggcgacgcgc gtgtcctggt tcgtcgtcgc gatgctgctg gcgctcgcgc
     3661 tcgcctgggg cgcgatcgag tggacgatca tccgccgcat cacgatgctc accaagcgct
     3721 cgcgcgccgt gacggcggcg ctggccggcg acgaggcggg gccgggccgc tggacggcgc
     3781 tggacgtcgc cgacctgcgc ggcccggacg agctgggcgt gctcgcggcc aacctcgccg
     3841 agctgatgca gcgcgtcgac gagagcatgc agcgcgagcg cctgcgcgcc gagcgcgaga
     3901 aggatcaatg gatggccgtg ggacacgaga tcatgtcgcc gctgcagtcg ctgatcgcgc
     3961 tgcacggcgg ccccgacgac ccgagccggc gctacatcct gcgcatgcag caggcgatcc
     4021 gcgtgctgta cggcagcgcg tcgccgagcg aggccttcca gtcgacgcag ctgcagctcg
     4081 cgacgctgga gctcggcgcc ttcctcgagg ccgtggccgc caacgcgccg cacgcgggca
     4141 tcgccgacgt ccgctacgag ggcccgtcgc accccgtcgt cgtgcgcgcg gacgaatacc
     4201 cgctcgagga cgtcgtcacc cacgtgctgc agaacgccca gcgccaccgg gtacccggca
     4261 cgccgatctc gatccgcctg cgcgcggacg acggcagcgc ggtcgtcgag ctgcacaacg
     4321 ccggcccgca catcccgccc gagctggtcg acaggatctt cgagtacggc gtctcggggg
     4381 atacggccag cggcatcgac gacggcgccg gcgccggcga ggaccgcgac cgcacggccc
     4441 cgcagcgcgg ccagggactg ttcgtcgcca agacctacat ggccaagatg ggcgggacga
     4501 tcgcggccga gaacgtcgcc gacggcgtgc gcatcgtgct gagcttcccg tgcgtcgtcg
     4561 cgtgacgcgc ggcgcccgaa tgttgcaggc gcgtcatgca ggacgcgagc aatgcaaccc
     4621 gggcggccgc gcgccggtgc gatagtgcgg acgtgtcgcg cgccgtccgc gcaggagtgc
     4681 tttccgtgac ctcgaaatcg ccgtccgcca ccatcgtccg cctcgcgcgg ctcgcgctgc
     4741 cggcgctcgt gctgctcgcc ctgctggccg ccggcacgtc ggcccaggcc cagcgcctgc
     4801 gcctgcgcga ggcactggcg atggacgacg gcaccgacga cgacgcgcgc gccgaggcgc
     4861 cggccggcac gcgcgtgctg cgcgatctcg catacggccc ggacgccgcg cagcgcctgg
     4921 acgtctacct gccgaaccag gcaccggcac ccggctcggc accgatgccg gtgatcctgt
     4981 tcgtgcacgg cggcggctgg gcgcgcggcg acaaggcgat gcaccgcgtc gtggatccga
     5041 aggtggggca ctgggtgggc cggggctggg cggtggtctc gatcgactac cgcatgctgc
     5101 cgacgcccgt ggcgacgcag gaggacgacg tcgccgccgc gctcgcgttc gcgcaggcgc
     5161 aggcaccgca gtggggcgcc gatccgaagc gcttcgtgct gatgggccac tcggccggcg
     5221 cccacctcgt cgcgctgctg gccgccggcg cgaggacgcc gtcgtcgccg cacctgcagc
     5281 cctggcgtgg cgcggtgttc ctggacagcg ccgccctgga cctgcccttc ctcatgacgc
     5341 accgccatct gcggctgtac gaccgcgcgt tcggcagcga cgcctccacg tggaccgcgc
     5401 tgtcgcccct ggccgttctc caggcaccgg cggcgccggt gctggcggtg tgctccacgc
     5461 ggcgcaccga gtcctgcatg gacgccgacc gcttcgccgc gaaggcgcgc tcgctcggcg
     5521 gccaggcgcg cgtgctgcgc gaggacctga gccacggcga gatcaacgcg acgctgggtg
     5581 cgccgtcgga ctacacgggg caggtggacg cgttcctcga ttcgctgcgg tgacttgccc
     5641 ccggattcgc agcggcggat tcaaccgggc ggcacgcaga cgggagagcg gtgacgcgcc
     5701 ctgcacgtcg gctaagctcc gcccatgccg tccgccgaac gtcgacgccc gcccccgcga
     5761 ctgcccctca tcgaccccgc ccgctgcacc ggttgcggcc gctgcgtcgc cgcctgcggc
     5821 ccgcgcgtgc tgagcctgga gacggtgcgc tggcgcaaga ccgcgacgct gcacgacgcg
     5881 gccggctgca ccggctgcag cctgtgcgag cggcggtgtc cgttcggggc gatcgcgatg
     5941 ggggcggcgc cttgaaggcc ggggctcgtt tcgagccaga ggaagatgat cgccgacgac
     6001 cgctttgagg tagcctactc gtcatgcgag atcccgctcg aattaatgtc gttctggctg
     6061 cgctgcgggc cgcgtggggg aatcacccga ccttcgcctc gggcagttga tcgtgaacgc
     6121 tgttcgaccc tcgaacccgt gcccggaagt cttccacgcg gacgacgatg cgcttcttcg
     6181 agggctggtc agttatcacg agatgttgca gctggccaag gcgagcaagg attccgaccg
     6241 ccgctgacgg gggcgaattc aaccggtcgt gaagtgcagc ttcgaggcgt gccgattgag
     6301 gccccagcgg atcgacgcgg attggagtca agatggatgt cctggcgatg cttttggtgt
     6361 gcgcggccat tgcctttccg atggcctccg tcttgatagc agcaaggctg tggcgagcaa
     6421 agctcgcacg accagcgcgt ttcaaggcag tgggactgac gctgctctac ctcttcaccg
     6481 gctatctgtt gacccgacca gtcccgaccc acgccgtagc gattgagccg ctcgacttca
     6541 ctcacccggt cgagaacatc aacggaagcg ttgcagtacg gtcagcgctt ctactgcttg
     6601 tggaggtagc cgcttcttcc atcgcggtcg tgcttttgct tcggcgcatc atgtcgcgtt
     6661 agagtcggca catggccgag gctgtgtgaa aactcgttgc cttgcgggca aggcgaacaa
     6721 tcgacgcgtc ggaacggtca tggttgcacg atggcgaccc cggcaagggt caagcgcccc
     6781 ccgaaaatgc tcgtccagcg agttttcaca cagcctcgac caggagccgc cgcccgaggt
     6841 cgcgggttga agggcgggaa ccggactgga agccgatgtt cacgagccac cggttgaatc
     6901 cgccggcagc ctgggacaaa ccacctgcgg ctgatgagct cctgagaaat ccgggccaag
     6961 caattgctgg cgtagcaggg catccagcac cgcatcttga gcaaacccaa tgtaaagccg
     7021 ctgtttatgg ggcagtcgcg cgacgacccc gataatcgtt tagcacctca acgaatcggt
     7081 ccaacaaggg ccggaatccc ataaaattaa tggcggcatc ctgcttcgcg acgacctcgt
     7141 aagcaaacgt cgctttcccg tacgttgtct tgctgtcgtt gtccttggat gcgtcaaagg
     7201 ttcgcccatt gaggacgacg gcttgcgtgg ctgcgtcgaa taggtcttcc atattggatg
     7261 atgtttttcc aaatggagtt ggcgtgagaa cgacgtagac gtttccaaag agatgatgaa
     7321 atccgacgga ttgatccaaa tccttaccga acatgtgctt gacatatgcg cgtatttttc
     7381 tgacgccgtc atcgtcatcg agcgtaatga ttacaggctg ctccccggga ggcgccttga
     7441 tagcttcgta ctcgcgatgc aggacttgga tgaactcttt gatgtcaggg gctcccccgt
     7501 gaattcctag cactctggcg gttagtcttc cgcgaccctt gattgggtaa cggtggcgcc
     7561 ggaatagaag tttgatcttt ccgtcagctg caacattagc tagttgcgga taacttgtcg
     7621 ctaaagagcg cgcagcctgc gcaatgtaaa ccaagtcagt cttaccttcg cacaggacaa
     7681 gcggcatcgg cggggcgtag aagtcatgga acaggacgaa cgtccggtac agtctttcag
     7741 gtgagttgag cggcgcggaa tagtcagcga cagtaccacg gcggcttgca tacacgatgt
     7801 actcgagcat cccccttaat tgctctggct tgccgacttc tttgacttct tgcaccgggc
     7861 caaccgaggc atcgcccttc cttttaaaga agaaggagcc cgtggaggtg taggtgtgaa
     7921 cgagcatccg tacatgacgt cggtactcat cgggagcatt tatttttcga ttgaccacga
     7981 gtccagtcac caactggcgc gagtcgtggt attgcatgcg ggtcttcgcc tcattcagcc
     8041 gaaagccgcc tcgatcgatc aaacgcttaa gggcgccacc gggcacccac ttgtgtgcca
     8101 ccgtctcgtc cgcgaccgca acggaaggag ggaacgcgca cttgttcgtt gagaaggtca
     8161 ggtcatcggc atatcgggag taagtgatgc cctccgcctt ggcaagtgat gtcaggtgga
     8221 tgtccactac atgcccgact aggttcgata tgaccggcga acatgggctt ccttgcggca
     8281 gcctgttctg gaagcaggca atttgcgcaa gcacggtggc gacaggagga tgcagggcga
     8341 agtctcgatc cttgatgaag aaccctcgga cccggccaaa attgatgctc ccgaagaagt
     8401 cttcaacgtc gacgttgaac acaaaacgct gcgcacgatg ttgcaaggcg ttggtgacga
     8461 ttgatcgacg acgccggaag ccgtgagaga tgcgatccgg tcgtttgctt ccatccgatt
     8521 ttccacgcct gaattgaatt tcttcaaggc tgtcctggag cactcccgcc aagttgcgct
     8581 gcagtagttt cagttgggcg cagggcgctg cgatggttcg catgccgccg gaacgctttg
     8641 ggacgctgaa ggtcgtgtac ttggcgtgtt ccgggagcct gtagaggagg taggccacag
     8701 ctcgtggagt gaagccgagc atccgggcga catcgtgaaa cgacacgcac gctttcaaat
     8761 ctgccagcgc cgacaagagc tttctcccgg aaaagggggc ctgtgagcgc tcctacgcca
     8821 gcggtgcggt aagtacgcat tgcttgaaga gaactgcagg gcatcgcccc actcattttt
     8881 gtcacgtgct ccgattcaga gcatcaaagt ccgctcacag gcccggtcag gatcgtagct
     8941 caacgccacg cagttctgcc ggacagctga tcgtagattc tgctgacccg tcgccatcgg
     9001 aagggatacg ttggactgca gcattctggc ggcgagttcg cgacagtgaa ggactgctcc
     9061 cgctggcgaa gcggctgctc aatccaacag cgcgagtggc gcagatgggt cgcaagccaa
     9121 cgtccacctc cgcaccccgc gaaccgccgc ccatgccgcc gccctccgaa gtcctcgcga
     9181 ggacttccag gccaggccat ccctcctgcg atcaccatcg acgcaccgag aaggcctgac
     9241 gcccggcatc ggagcccggc cgtccaggcc cgcgcaggcg acgcccgggc tggcaggtcg
     9301 gcgatcccgc cggcctggtg cggcgcccga gcgcggaggt cgaccgcccg ggccgcgagg
     9361 tccgccgatg gactgcgggg cgagaccgtc cgggctgcga agtccaccgc cggactgatc
     9421 cggtgcagcg acgggcccct gaagcggaaa gggccgcccg aaggcagccc tttcgcatgc
     9481 ggcggcgagc ccgcctcgcc gcgccgtcag gcggccggcg tcgcctgcac cgcgttgcgc
     9541 aggcggatgt gcagctcgcg cagctgcttc tcgtcgacca tgctgggcgc gccggtcagc
     9601 aggtcctgcg cgcgctgggt cttcgggaag gcgatcacgt cgcggatcga ctcggcgccc
     9661 gtcatcatcg tgacgatgcg gtcgagaccg aacgcgaggc cgccgtgcgg gggcgcgccg
     9721 tactgcagcg cgtcgagcag gaagccgaac ttgacctgcg cgtcctcgcg gctgatgttc
     9781 aacgccgaga agaccttgct ctgcacgtcg gcgcggtgga tccgcaccga gccgccgccg
     9841 agttcccagc cgttgagcac catgtcgtag gccttggcga tgcaggcgcc cgggtcgctg
     9901 tccatcaggt cctcgtggcc gtccttcggc gcggtgaacg ggtggtggac cgcgttccag
     9961 cggttctcct cgtcgtcgcg ctcgaacatc gggaagtcga cgacccacag cggcgcccac
    10021 ttgtcctcga acaggccgtt ggccttgccg aactccgagt ggccgacctt caggcgcagc
    10081 gcgcccatcg cgtcgttgac gatcttggcc ttgtcggcgc cgaagaagat caggtcgccg
    10141 tcgaccgcgc ccgtgcggcc gaggatctcg gcgatcgcgg cgtcgtgcag gttcttgacg
    10201 atcggcgact gcaggccctc gcggcccttc gagacgtcgt tgaccttgat ccaggcgagg
    10261 cccttggcgc cgtagatctt cacgacctcg gtgtaggcgt cgatctcgcc gcggctgatc
    10321 tccgcgccct tgggcacgcg cagcgcgacg acgcggccgc cggccatctg cgcggccgac
    10381 gagaacacct tgaagtcgac gtcgcccatg acgtcggtca gctcggtgaa ctccagcttc
    10441 acgcgcaggt cgggcttgtc cgagccgtac aggcgcatcg cttcggcgta ggtcatcacc
    10501 gggtacacgc ccaggtcgac gccgatcgcg ttcttgaaga ccgtgcggat catgccctcg
    10561 aacatcgcgc ggatctcctc ctcggtgagg aacgaggtct cgatgtcgat ctgcgtgaac
    10621 tcgggctggc ggtcggcgcg caggtcctcg tcgcggaagc acttcgtgat ctggtagtag
    10681 cggtcgaagc ccgcgaccat cagcagctgc ttgaacagct gcggcgactg cggcagcgcg
    10741 aagaagtggc cgtcgtgcac gcggctcggc acgaggtagt cgcgcgcgcc ctcgggcgtg
    10801 ctcttgccca gcatcggcgt ctcgatgtcc acgaagccct gggcgtcgag gaacttgcgc
    10861 acctccatgg cgacgcggta gcgcagcatc aggttcttct gcatctgcgg gcggcgcagg
    10921 tccagcacgc ggtgtgtgag gcgcgtggtc tccgacaggt tctcgtcgtc gagctggaac
    10981 gggggcgtga cgctcgggtt gagcacctcg agctcgtggc acagcacctc gaccttgccg
    11041 ctggcgatgt tggcgttttc cttgtcgccc gggcgcgcgc gcacgaggcc gacgaccttc
    11101 aggcagaact cgttgcgcac gccctcggcg gtctggaaca tctcggggcg gtcggggtcg
    11161 cagacgacct gaacgtggcc ttcgcggtcg cgcagctcga tgaagatgac gccgccgtgg
    11221 tcgcggcggc ggtgcgccca gcccatcagg gtcacggtct ggccgatcag cgcttcgctg
    11281 acgaggccgc agtaggtggt tcgcattggc agtactccgt gttcttgtgc gttcggatgg
    11341 ccgccggcgc gccctgtggc gggcgaggcg cggcagccgg gaatctggat ggaatcaggc
    11401 cggggtcggg gggctaccct caggactgaa cgccgatgac ctcggggcgt tcagcccacc
    11461 cgattggggt cggccccctc cggcacgacc aggaggttcg gtgccggggg cgcgacgacg
    11521 cccatcgaga tgatgtactt cagggcctcg tcgacgctca tcgacagggg cttgcaggcc
    11581 gcccgcggca tgatcaggaa gaagccggag gtcgggttcg gcgtggtcgg gacgtagagg
    11641 ctcacgtgct ccgcgggcag cgccgccgcg acctcgcccg agggccggcc ggtgacgaag
    11701 gcgatcgtcc agctgccggg gtgcggatac tgcacgagca ccgcctcgcg gaaggcgttg
    11761 ccgttggtgg agaacagcgt gtcggagacc tgcttgaccg acacgtagat cgacttgacg
    11821 atcgggatgt gcgacagcag ccggttccac tgcgcgagcc accaggcgcc gaccatgttc
    11881 gtcacgaaca tgcccgtcag cagcagcagg agcagcagca gcacgacgcc caggccgggc
    11941 acgccgccca gcagcgcgat gaagttgtcg gcgcggtccg gcaggacgtg ctgcgccgtc
    12001 gcgaggatgc cgtggaagac gccatccatc agcccgacgg cccacatcag cacgccgacg
    12061 gtgatcgcga gcggcagcca caccagcagg ccggcgaaga ggtacttcct caagagaggc
    12121 tcccgtcgtg gtcgtcgtgg aggcggtggg ccgcgcggga cgtcatcgcg gaggcgccgt
    12181 caatggcagg cgcaaccgcc gccgcaggcg ccggaggacg ccgcggccga ggcaccgccg
//
LOCUS       DASVLN010000485         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_239211, whole genome shotgun sequence.
ACCESSION   DASVLN010000485 DASVLN010000000
VERSION     DASVLN010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239211"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1329)
                     /locus_tag="VF453_11955"
     CDS             complement(31..1329)
                     /locus_tag="VF453_11955"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019254972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX7688417.1"
                     /translation="MMMPQQVLLRDAMRRLDMTRDAFADRIGSRRRALDSWLLPDDSS
                     EFRAMPEVARRFVQEIVAHAAAHPQPTQSVHVDEPTGPAASGPMRHLLSIDQFDREAV
                     ERLFALADVMQPVARRRKVTRVLEGAVLANLFFEASTRTRVSFGAAFCRLGGSVCDTT
                     GFTFSSMAKGESIHDTSRVISGYVDAIVVRHPEQGAVAEFARATNVPVVNGGDGPGEH
                     PTQALLDLYTIQREFSRLGKRVDGAHVALVGDLKYGRTVHSLIKLLAMYRGMRFTLVA
                     PPSLEMPGALVDRVARRGNAVEVRTSLADGLRDADIVYTTRIQKERFAGESLESIEGA
                     LGDYRIDRAAVDACCRPDVVVMHPLPRDSRAGAHDLATDLDADPRLAIFRQTDNGIPV
                     RMAIFAQLLGVDHLVQRSMRDAAWTPPEHIGPEDALLPGE"
     gene            complement(1572..2123)
                     /locus_tag="VF453_11960"
     CDS             complement(1572..2123)
                     /locus_tag="VF453_11960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7688418.1"
                     /translation="MQRHFLFLVASARQPGTIGNTEWLARQAARHLPAGATAQWRHLN
                     EAEIQAFEDRRHTGDGTYPMPVGDAKALLDATLAATDLVFVTPLYWYSLPHRLKLYLD
                     HWSAWMRVPGLEFKARMQGKRAWAVTTSGSREKTQPMFDSLKLCAEFMAMDWRGALWG
                     AGGAPGAIEKDAAALAAAGDFFA"
     gene            2157..>2774
                     /locus_tag="VF453_11965"
     CDS             2157..>2774
                     /locus_tag="VF453_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEX7688419.1"
                     /translation="MNAPASPAPLPPLGEADVDRLQVLLDKIPEPLEPLDVSMLDGYL
                     CGVLLQPAAVPAGAWLKHVFDVDGRPLPDKWAGQHELQGLVQRRFQELSRAIAGRQWF
                     DPWVFELDDEATPAEASLPWVAGFAAAMELFPALMDMDAPEAIEPLAVLYAVFDPEDL
                     EDADELLEVIEELEPAATLDEAIEDIVQSVLRLADVGRPLPAGKRP"
BASE COUNT          397 a          988 c          986 g          403 t
ORIGIN      
        1 gacgccgcgg cgcggcgtga ggcccgggcg tcactcgccc ggcagcagcg cgtcctccgg
       61 gccgatgtgc tcgggcggcg tccaggcggc gtcgcgcatc gagcgctgca cgaggtggtc
      121 gacgcccagc aactgcgcga agatcgccat ccgcaccggg atcccgttgt ccgtctggcg
      181 gaagatcgcg agccgcgggt cggcgtcgag gtcggtcgcg agatcgtgcg cgcccgcgcg
      241 gctgtcgcgc ggcagcgggt gcatcacgac gacgtcgggg cggcagcagg cgtcgacggc
      301 agcgcggtcg atgcggtagt cgcccagcgc gccctcgatc gactccagcg actcgcccgc
      361 gaagcgctcc ttctggatgc gcgtcgtgta gacaatgtcg gcgtcgcgca gtccgtcggc
      421 gagcgacgtg cgcacctcga ccgcgttgcc gcgccgcgcg acgcggtcga cgagcgcgcc
      481 gggcatctcc agagacggcg gcgccacgag cgtgaagcgc atcccgcggt acatcgcgag
      541 cagcttgatc agcgagtgca ccgtgcgtcc gtacttcagg tccccgacca gggcgacgtg
      601 tgcgccgtcc acgcgcttgc ccaggcgcga gaactcgcgc tggatcgtgt agaggtccag
      661 cagcgcctgc gtcgggtgct cgccggggcc gtcgccaccg ttgacgaccg gcacgttggt
      721 cgcccgcgcg aactccgcga ccgcgccctg ctccggatgg cgcacgacga tcgcatcgac
      781 gtagccgctg atcacccggc tcgtgtcgtg gatcgactcg cccttggcca tcgacgagaa
      841 cgtgaacccg gtcgtgtcgc acaccgagcc gccgaggcgg cagaacgcgg cgccgaagct
      901 cacgcgcgtg cgcgtcgagg cctcgaagaa caggttcgcg agcaccgcac cctcgagcac
      961 gcgggtgacc ttgcggcggc gcgcgacggg ctgcatcacg tcggcgagcg cgaacagccg
     1021 ctccaccgcc tcgcggtcga actggtcgat cgacagcagg tgccgcatcg gcccggacgc
     1081 cgccggccct gtcggctcat cgacatgtac gctttgcgta ggctgcggat gagcggccgc
     1141 atgggccacg atctcctgca cgaagcgccg tgccacctcc ggcatggcgc ggaattcgct
     1201 ggaatcgtcc ggcagcagcc aggaatcgag cgctcgccgc cggctgccga tgcgatcggc
     1261 gaaggcgtcg cgggtcatgt cgaggcggcg catcgcgtcg cgcaggagga cttgctgagg
     1321 catcatcatg tcgcgattgt ctacgcaaag cgtatatgcg cacaagccgg ccgaggcaaa
     1381 aaaaacccgc cgggggcggc gggttccaag cccttcgcga gaaggacgtc gttgggactg
     1441 attgcgtccg gtcagccacg ccctcctccc agaaagcacc gatcgccgaa cgcaacgcca
     1501 gtcaatgtag gcggcgaccc ggcggccgcc atcccccctt cgagggatgg gaactagggg
     1561 gtgggcgggt ctcaggcgaa gaagtcgccg gcggccgcca gcgccgcggc atccttctcg
     1621 atcgcgccgg gcgcgccgcc ggcgccccac agcgcgccgc gccagtccat cgccatgaac
     1681 tcggcgcaca gcttcaggct gtcgaacatc ggctgcgtct tctcgcggct gccgctggtg
     1741 gtgaccgccc aggcgcgctt gccctgcatg cgcgccttga actcgaggcc gggcacgcgc
     1801 atccaggcgc tccagtggtc gaggtacagc ttgaggcgat ggggcaggct gtaccagtac
     1861 agcggcgtga cgaagacgag atccgtcgcc gcgagcgtgg cgtccagcag cgccttcgcg
     1921 tctcccacgg gcatcgggta ggtgccgtcg ccggtgtggc ggcgatcctc gaaggcctgg
     1981 atctccgcct cgttcaggtg ccgccactgc gccgtcgcgc ccgcgggcag gtggcgtgcg
     2041 gcctggcggg cgagccattc ggtgttgccg atcgtcccgg gctggcgggc gctcgcgacg
     2101 aggaacagga agtgtctttg catcgcggca tcatctcatc ggttcacaat gccctcatga
     2161 acgcgcccgc ttcccccgct ccgctgccgc ccctgggcga ggccgacgtc gaccgcctgc
     2221 aggtgctgct cgacaagatc cccgagccgc tcgagccgct ggacgtcagc atgctcgacg
     2281 gctacctctg cggcgtcctg ctgcagcccg ccgcggtgcc ggcgggcgcg tggctgaagc
     2341 acgtcttcga tgtcgacggc cgtcccctgc ccgacaagtg ggccggccag catgagctgc
     2401 agggcctggt gcagcggcgc ttccaggagc tctcgcgcgc catcgccggg cgccagtggt
     2461 tcgacccctg ggtgttcgag ctcgacgacg aggccacgcc cgccgaggcg tcgctgccct
     2521 gggtcgcggg ctttgccgcg gcgatggagc tgtttcctgc gctgatggac atggacgcgc
     2581 ccgaggccat cgagcccctg gccgtgctct acgccgtctt cgaccccgag gacctggagg
     2641 atgccgacga gctgctcgag gtgatcgagg agctcgagcc cgccgccacg ctggacgagg
     2701 cgatcgagga catcgtccag agcgtcctgc gcctggccga cgtcggccgc ccgctgccgg
     2761 ccggcaagcg ccct
//
LOCUS       DASVLN010000486        12310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_510095, whole genome shotgun sequence.
ACCESSION   DASVLN010000486 DASVLN010000000
VERSION     DASVLN010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12310
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510095"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /gene="rnr"
                     /locus_tag="VF453_11970"
     CDS             complement(<1..2096)
                     /gene="rnr"
                     /locus_tag="VF453_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX7688420.1"
                     /translation="MGEVEGIVVGHRDGHGFVKRDDGEPDVYLSQQEMRSVLHRDRVR
                     VRLVRFDRKGRPEGRVLEIVERRKAPIIGRLLNEGGAWLVAPEDRRYGQDILIPKNAT
                     AAAVAGQVVAVELTEPPSMYSQPVGRITEVLGEIDDPGMEIEIAVRKYEVPHRFSPET
                     LAQTASLPDHVRAVDTRGRIDLTDVALVTIDGEDARDFDDAVYCEPHKSGRGKSAFAG
                     WRLVVAIADVSHYVKPGEPLDADAYERATSVYFPRRVIPMLPEKLSNGLCSLNPEQDR
                     LSMVCDMLIDGDGELHAYQFYPAVIRSHARLTYTEVAAVLGNTRGPEAAQRAELVPHL
                     LNLHDVFRALLKARHRRGAIDFETTETQIVCDDNGRIERIVPRTRNDAHRLIEEAMLA
                     ANVCAADFIAMHKHPALYRVHEGPTPEKKATLQNYLRALGLGLSISDDPKPAEYQALA
                     EATKDRVDAQQIHSMLLRSMQQAIYTPHNSGHFGLAYQAYAHFTSPIRRYPDLLVHRV
                     IKAILGSRRYHWQAPKVENSAAAGMRKVSAPVPARATSKSQAAEMEQWMGAGDHCSAN
                     ERRADEASRDVEAWLKCRFMRERLGEEFSGTVSAVTSFGLFVTLDTLYVEGLVHITEL
                     GGEYFRFDEVRQELRGERTGIRYAIGARVRVQVSRVDLDGRKIDFRVVREAESERLLA
                     RGRRSGAGEGARAAVSS"
     gene            2210..2294
                     /locus_tag="VF453_11975"
     tRNA            2210..2294
                     /locus_tag="VF453_11975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2244..2246,aa:Leu,seq:cag)
     gene            complement(2518..3042)
                     /locus_tag="VF453_11980"
     CDS             complement(2518..3042)
                     /locus_tag="VF453_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX7688421.1"
                     /translation="MLTDDGKHLYISWDEYHRLIEKLALIVHESGWEFDQILCLARGG
                     MRPGDMLSRIFDKPLAIMATSSYRAEAGTIQGRLDMAKYITLPSGELAGRVLLVDDLS
                     DSGVTLAAVVERLRGMPSIRELRSAVLWTKAVSTYTPDYFVEKLPTSPWIHQPFEDYD
                     GIAIETLAKRHEEA"
     gene            complement(3083..4423)
                     /locus_tag="VF453_11985"
     CDS             complement(3083..4423)
                     /locus_tag="VF453_11985"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX7688422.1"
                     /translation="MQQGNTPLTRGRNVVVVGTQWGDEGKGKVVDWLTTHAQGVVRFQ
                     GGHNAGHTLVIKGVKTALQLIPSGVMRDGVKCYIGNGVVVDPTHLISEIERLEKIGLD
                     VRSRLYVSEACPLIMPFHVAVDRAREAQREQCGDGKIGTTGKGIGPAYEDKVARRALR
                     MQDLKHPERFAKKLRDLLELHNATLERLGADKLEFQAIFDQAMQVAEQLKPMMSDVGY
                     SLYKANQAGANLLFEGAQGTLLDIDHGTYPFVTSSNCVAGNAAAGTGLGPDHLQYILG
                     ITKAYTTRVGSGPFPTELDIAQEGGVGHHLSTVGQERGTVTGRARRCGWLDAAALKRA
                     VIINGLSGLCITKLDVLDGLREIKVCVGYKLDGQDVDILPSDADDVARCEPVYETYPG
                     WSESTFGITEWEKLPLNARRYLERVKALIGAPIDMVSTGPDRDHTILLRHPYQA"
     gene            complement(4504..5682)
                     /locus_tag="VF453_11990"
     CDS             complement(4504..5682)
                     /locus_tag="VF453_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEX7688423.1"
                     /translation="MTSAWLLPEHIADVLPSEARRIEELRRKLLDLARGYGFELVIPP
                     MLEHLESLLSGTGHALDLKTFKLVDQLSGRMLGVRADTTPQVARIDAHLLNRQGVARL
                     CYCGPALHARPERPLATRELLQFGAEIYGHAGLEADLEIQDLALDGLAAAGVGSLVLD
                     LGDARIVRTLLAGEGGAGAIAPELQAQIAAALTLKDRSELEAVTATCAPATRGALVAL
                     LDLYGGSEVLDEARRRLPAHPTIAAALDDLAWLAAHVRTAHPGIEIGFDLSDMSGYAY
                     YSGVRFSVYAPGSTDALLRGGRYDEVGAVFGRNRPAVGFSLELRALSAAMPALPLKAA
                     IRAPWSEDAGLRAAIRALRAADETVVCALPGHEHETQEFECDRELVAVGNEWQLRSLA
                     "
     gene            complement(5823..6011)
                     /locus_tag="VF453_11995"
     CDS             complement(5823..6011)
                     /locus_tag="VF453_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX7688424.1"
                     /translation="MGDLFLSAVALVLVFEGMVPFVSPGTWRRVFERAVKMTDHQIRL
                     FGLASLALGAGLLVLFRG"
     gene            complement(6022..6921)
                     /gene="hflC"
                     /locus_tag="VF453_12000"
     CDS             complement(6022..6921)
                     /gene="hflC"
                     /locus_tag="VF453_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX7688425.1"
                     /translation="MNRIFMPLIGLLLVLLVASSSLFVVDQKHVAVIKTFGEIRSVVT
                     EPGLQFNWPLVQTVSMLDKRIQTLSSSEPRAIFTAEKQSLVVDWMVKWRISDARQYVR
                     NNGIEQHNLENRLGAVVQAAFNEEVTRHTVRGVLSDEREQIMQNVQARLAQDTKSFGI
                     EIVDVRIKRVDFVAEITDAVYKRMISERTQVANQLTAEGEAVKEQIRADADKQRAVII
                     ANATLDAQKIKGEADAQATQLYGEAFGRDPRFASFYRSLQAYRASFRKSDIVVVDTGS
                     EFFKAMRGSGSEAADAGAPARKR"
     gene            complement(6932..8218)
                     /gene="hflK"
                     /locus_tag="VF453_12005"
     CDS             complement(6932..8218)
                     /gene="hflK"
                     /locus_tag="VF453_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX7688426.1"
                     /translation="MPENTHSSRRASWRAALRKALVPGLRAQGQDGGPPDLDELWRDF
                     TTKLARFFGVGNRKGDAGGGNGGGFQADPRSTGMGIAAVLLIVVIVWLFSGSFIVQEG
                     QRAVVTTFGRYSHTVGAGWGWRWPWPIQDNETINFTQLRTVEVGGNGVSSATGLHDSA
                     MLTQDENIVDVRFNVQYSLKDARDFLFNNDHPDTAVQQAAESAVREIVGRSRIDSILY
                     EQREALPQALAKSIQAQLDRLNAGVFVAKVNIENVTVPEQVTQAFQDVIKAGVDRDRL
                     KNEGQAYANQVIASAKGDAARLHEDALGYKARIVGAAEGDSDRFKAILAEYQKAPAVT
                     RDRMYIDAMTEVYANVAKVMVDTHGGTNLIQLPLDKLAQASAANGASSSSPGGGSGAS
                     ASATPSVTVTPVDPSSASAIDSRARDASRARDRDTR"
     gene            complement(8221..9483)
                     /gene="hflX"
                     /locus_tag="VF453_12010"
     CDS             complement(8221..9483)
                     /gene="hflX"
                     /locus_tag="VF453_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7688427.1"
                     /translation="MAAPPRAVLVGVDFGRGAPFDDTLDELALLAESAGDLPVARVIA
                     RRKAPDPALFVGSGKADEIKELVLAHRAQGVLFDQALSPAQQRNLERHLGVGVADRTA
                     LILEIFAARAQSHEGKLQVELARLQYLSTRLVRRWSHLERQQGGAGVRGGPGETQIEL
                     DRRMIGDRIKSVKERLVKVKRQRQTQRRSRERSGTFRVSLVGYTNAGKSTLFNALVKA
                     NAYAANQLFATLDTTTRQMYLGEVAESVSVSDTVGFIRDLPHKLVEAFRATLQEATDA
                     DLLLHVIDAASPVLQEQRDEVERVLAEIGAADVPQVLVYNKLDRLDAASRPETLVDEL
                     EGEHGVRVPRVFVSALTGEGLDALRATIAQAIVASREAPREDAPRDARFARPDDEPAD
                     SDDELADSDDNAPDDDPGPRASPPSSDA"
     gene            complement(9599..9835)
                     /gene="hfq"
                     /locus_tag="VF453_12015"
     CDS             complement(9599..9835)
                     /gene="hfq"
                     /locus_tag="VF453_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX7688428.1"
                     /translation="MSNKGQLLQDPFLNLLRKEHVPVSIYLVNGIKLQGHIESFDQYV
                     VLLRNTVTQMVYKHAISTVVPGRAVNFHPGDAAE"
     gene            complement(9925..11268)
                     /gene="der"
                     /locus_tag="VF453_12020"
     CDS             complement(9925..11268)
                     /gene="der"
                     /locus_tag="VF453_12020"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX7688429.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTKTRDALVADFAGLTRDRHYGD
                     GRHGDIEFIVIDTGGFEPDSSTGIVKEMARQTRQAVAEADIVIFVVDVRGGLSAQDHD
                     IAKYLRVSQKKVLLAVNKAEGMSESPALAEFHELGMGELHPISSAHGQGVRSLLDAAF
                     EGLEAAPEHDEFGAGPGDRAIRLAVAGRPNVGKSTLINTWLGEERLVAFDMPGTTRDA
                     ITVPFEREGHRFELIDTAGLRRKGRVFEAIEKFSVVKTLQAIADANVVVLLLDATQGV
                     TDQDAHIAGYILDSGRAVVIAINKWDAVDSYQREMLQRSIDQRLAFLKFAPAVNISAL
                     KRQGLGPLWKNIADAHASANRKMTTPVLTRLLQEAVKHQQPPRAGMSRPKMRYAHQGG
                     MNPPIIVIHGNALEHVPEAYKRFLEGRFREHFKLVGTPLKLEMRSGRNPFTDKEE"
     gene            complement(11375..>12310)
                     /locus_tag="VF453_12025"
     CDS             complement(11375..>12310)
                     /locus_tag="VF453_12025"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7688430.1"
                     /translation="KDFVIAALNGTVVSVDADSGKENWRADAGGSLSSGVGTDGRFAA
                     VVTRGGELVTFEGGKVKWRQHLQSAVVTAPFVAGERVFTLGVDRSVNAFDALDGTRIW
                     TYAKPGDALTLSQAGVLTSYQDTLLAGQGARLTALDPLKGTVRWEVPVAAPRGTNEVE
                     RLADLVGPAARVGNVYCIRAFQNGIGCVDAERVTALWSINAGGSQPVGADEDYVFAAD
                     GSDRLSARRRSNGESVWLSDKFQNRRLSGMLSIGTVLVFGDLQGWVHFLDRKTGDPLL
                     RLETDGSPIVGTPVRAGNTIAVTTKSGGVFAFRPE"
BASE COUNT         1707 a         4194 c         4417 g         1992 t
ORIGIN      
        1 gaggacaccg ccgcccgcgc gccctcgccc gcgcccgacc tgcggccgcg cgccagcagc
       61 cgctcgctct cggcctcgcg cacgacgcgg aagtcgatct tgcggccgtc caggtcgacg
      121 cggctgacct gcacccgcac ccgcgcgccg atcgcgtagc ggatgcccgt gcgctcgccg
      181 cgcagctcct ggcgcacctc gtcgaagcgg aagtactcgc cgccgagttc ggtgatgtgc
      241 accagcccct cgacgtagag cgtgtccagc gtgacgaaca gcccgaagct cgtgacggcg
      301 ctgacggtgc cggagaactc ctcgcccagg cgctcgcgca tgaagcggca cttgagccag
      361 gcctcgacgt cgcgcgaggc ctcgtcggcg cggcgctcgt tggcgctgca gtggtcgccc
      421 gcgcccatcc actgctccat ctcggcggcc tgcgacttcg acgtcgcgcg cgccggtacc
      481 ggcgccgaca ccttgcgcat gccggcggcg gcggaattct cgaccttcgg cgcctgccag
      541 tggtagcgcc tcgagccgag gatcgccttg atcacgcgat gcaccagcag gtcgggatag
      601 cgccggatcg ggctcgtgaa gtgcgcgtag gcctggtagg cgaggccgaa gtggccgctg
      661 ttgtgcggcg tgtagatcgc ctgctgcatc gagcgcagca gcatcgagtg gatctgctgg
      721 gcgtccacgc gatccttggt cgcctcggcg agcgcctggt actcggcggg cttgggatcg
      781 tcgctgatcg acaggcccag gcccagcgcg cgcaggtagt tctgcagcgt ggccttcttc
      841 tcgggcgtcg ggccctcgtg cacgcggtac agggccgggt gcttgtgcat cgcgatgaag
      901 tcggccgcgc agacgttggc cgcgagcatg gcctcctcga tcagccggtg cgcgtcgttg
      961 cgcgtgcgcg ggacgatgcg ctcgatgcgg ccgttgtcgt cgcagacgat ctgggtctcg
     1021 gtcgtctcga agtcgatcgc gccgcgccgg tggcgcgcct tgagcaacgc gcggaagacg
     1081 tcgtgcaggt tcagcaggtg cggcaccagc tcggcgcgct gggccgcctc gggcccgcgc
     1141 gtgttgccca ggacggccgc gacctcggtg taggtcaggc gcgcgtgcga gcggatcacg
     1201 gccgggtaga actggtacgc gtgcagctcg ccgtcgccgt cgatcagcat gtcgcacacc
     1261 atggacaggc ggtcctgctc ggggttgagc gagcacaggc cgttcgagag cttctccggc
     1321 agcatcggga tgacgcggcg cgggaagtag accgaggtcg cgcgctcgta ggcgtcggcg
     1381 tccagcggct cgccgggctt gacgtagtgg ctgacgtccg cgatcgcgac gaccaggcgc
     1441 cagccggcga acgcgctctt gccgcggccg gacttgtgcg gctcgcagta gaccgcgtcg
     1501 tcgaagtcgc gcgcgtcctc gccgtcgatc gtgacgagcg cgacgtcggt caggtcgata
     1561 cggccacggg tatcgacggc acgcacgtgg tcgggcagcg aggcggtctg cgcgagcgtc
     1621 tcgggcgaga accggtgcgg cacctcgtac ttgcgcaccg cgatctcgat ctccatgccg
     1681 gggtcgtcga tctcgcccag cacctcggtg atgcggccga cgggctgcga gtacatcgac
     1741 ggcggctcgg tgagctccac cgccaccacc tggccggcga ccgctgcggc ggtggcgttc
     1801 ttcgggatca ggatgtcctg gccgtagcgg cgatcctccg gcgccacgag ccaggcgccg
     1861 ccttcgttga gcagccgtcc gatgatgggc gccttgcggc gctcgacgat ctccagcacc
     1921 cgcccttcgg gacggccctt gcggtcgaag cgcacgaggc gcacccgcac ccggtcgcgg
     1981 tgcagcacgg agcgcatctc ctgctgcgag aggtagacgt cgggctctcc gtcgtcgcgc
     2041 ttgacgaatc catggccgtc gcgatgaccg acgacgatgc cctcgacctc acccatgagg
     2101 gccccggaaa gcgccgaggg gcttgcaact tgttttgttt tgatggtata gtctctttct
     2161 tcgctgatta ctgattgtct tcgacaacgg tggttgacga aagtgccttg cccagatggc
     2221 ggaattggta gacgcactag tttcaggtac tagcgcttaa cggcgtggag gttcgagtcc
     2281 tcttctgggc accatattcg agaaggcctt gcgaacggaa gtttgcaagg cctttttctt
     2341 tgtcgtcgcg caggacggcg agggacggat gcggcgtcgg catgacgcgc actgtatccg
     2401 acatcgcgaa ggcctcgagc gcacccccgg gccgcgtgaa gcgacgccgc ggcggccact
     2461 gcgccgggcc gccgcgacgc gcccggcgca tggtgaaggc gtccctcgca gccagcctca
     2521 ggcttcctcg tgccgcttcg cgagcgtctc gatcgcgatg ccgtcgtagt cctcgaacgg
     2581 ctggtggatc cacgggctcg tgggaagctt ctcgacgaag tagtcgggcg tgtacgtcga
     2641 gacggccttg gtccacaaca ccgcggagcg cagctcgcgg atcgagggca tgccgcgaag
     2701 gcgctcgacc accgcggcca gcgtcacgcc cgagtcggag aggtcgtcca ccagcagcac
     2761 ccgtcccgcc agctcgccgc tgggcagcgt gatgtacttg gccatgtcca ggcgcccctg
     2821 gatcgtgccg gcctcggcgc ggtacgagct cgtggccatg atggccagcg gcttgtcgaa
     2881 gatgcgcgag agcatgtcgc ccgggcgcat gccgccgcgc gcgaggcaca ggatctggtc
     2941 gaactcccag cccgactcgt gcacgatcag cgcgagcttc tcgatcagcc ggtggtattc
     3001 gtcccaggag atgtacaggt gcttgccgtc gtccgtgagc atcttcgtcc ctctatccaa
     3061 gatgttgcgc gggtgaatcg tttcaagctt gatacgggtg ccgcagcagg atcgtgtggt
     3121 cgcgatccgg gcccgtggac accatgtcga tgggcgcgcc gatcagcgcc ttgacgcgct
     3181 ccaggtagcg gcgcgcgttg agcggcagct tctcccactc ggtgatgccg aaggtcgact
     3241 cgctccagcc cggataggtc tcgtagaccg gctcgcagcg ggcgacgtcg tcggcgtcgc
     3301 tgggcaggat gtcgacgtcc tggccgtcga gcttgtagcc gacgcagacc ttgatctcgc
     3361 gcaggccgtc gaggacgtcc agcttcgtga tgcacaggcc cgacaggccg ttgatgatga
     3421 cggcgcgctt cagggcggcc gcgtccagcc agccgcagcg gcgggcgcgg ccggtgacgg
     3481 tgccgcgctc ctggccgacc gtcgacaggt ggtggccgac gccgccttcc tgcgcgatgt
     3541 ccagctcggt cgggaacggg cccgagccga cgcgcgtcgt gtaggccttg gtgatgccga
     3601 ggatgtactg caggtggtcg gggcccaggc ccgtgccggc cgcggcattg ccggccacgc
     3661 agttgctcga ggtgacgaac ggataggtgc cgtggtcgat gtccagcagc gtgccctgcg
     3721 cgccctcgaa cagcaggttc gcgcccgcct ggttggcctt gtagaggctg tagccgacat
     3781 cggacatcat cggcttcagc tgctcggcga cctgcatcgc ctggtcgaag atggcctgga
     3841 actcgagctt gtcggcgccc aggcgctcca gcgtggcgtt gtgcagctcg agcaggtcgc
     3901 gcagcttctt ggcgaagcgc tcggggtgct tcaggtcctg catgcgcagc gcgcggcggg
     3961 ccaccttgtc ctcgtaggcc gggccgatgc ccttgccggt cgtgccgatc ttgccgtcgc
     4021 cgcactgctc gcgctgcgcc tcgcgcgcgc ggtcgaccgc gacgtggaac ggcatgatca
     4081 gcggacaggc ctcgctcacg tacagccgcg agcgcacgtc caggccgatc ttctcgaggc
     4141 gctcgatctc cgagatcagg tgcgtcggat cgacgaccac gccgttgccg atgtagcact
     4201 tcacgccgtc gcgcatcacg cccgagggga tgagctgcag cgccgtcttg acccccttga
     4261 tgaccagcgt gtggcccgcg ttgtgcccgc cctggaagcg cacgacaccc tgcgcgtgcg
     4321 tggtcagcca gtcgaccacc ttgcccttgc cttcgtcacc ccattgggtg ccgacgacga
     4381 cgacgttgcg gccgcgcgtg aggggagtgt tgccttgttg catggaagtg gttcaggagt
     4441 tgggaacgcc gccttcgcgg gcgcgcgttc ggatcgggaa aggacggttt gccggagggc
     4501 gcctcaggcg aggctgcgca gctgccactc gttgccgacg gcgaccagct cgcggtcgca
     4561 ttcgaattcc tgcgtctcgt gctcgtgccc gggcagcgcg cagacgacgg tctcgtctgc
     4621 ggcccgcagc gcgcggatcg cggcgcgcag gccggcgtcc tcgctccagg gcgcgcggat
     4681 ggcggccttg agcggcagcg cgggcatcgc cgcggacagc gcccgcagct ccaggctgaa
     4741 gccgaccgcg ggccggttgc ggccgaagac ggcgccgacc tcgtcgtagc ggccgccgcg
     4801 cagcagcgcg tcggtcgagc cgggcgcgta gaccgagaag cgcacgccgc tgtagtaggc
     4861 gtagccgctc atgtccgaca ggtcgaagcc gatctcgatg cccgggtgcg ccgtgcgcac
     4921 atgggccgcg agccaggcga ggtcgtccag cgccgcggcg atcgtcggat gcgcgggcag
     4981 gcggcgccgc gcctcgtcca gcacctcgga gccgccgtac aagtccagca gcgcgaccag
     5041 cgcgccgcgc gtcgccggcg cgcaggtggc cgtgacggcc tcgagctcgc tgcgatcctt
     5101 gagcgtgagg gccgcggcga tctgcgcctg cagctccggc gcgatggccc cggcgccgcc
     5161 ctcgcccgcc agcagcgtgc gcacgatgcg cgcgtcgccg aggtcgagca ccagcgagcc
     5221 cacgcccgcg gcggccaggc cgtcgagcgc caggtcctgg atctcgaggt cggcttccag
     5281 gccggcgtgg ccgtagatct cggcgccgaa ctgcagcagc tcgcgcgtgg cgagcgggcg
     5341 ctcggggcgc gcatgcagcg cggggccgca gtagcacagg cgggcgacgc cctggcgatt
     5401 gagcagatgg gcgtcgatgc gcgcgacctg cggggtggtg tcggcacgca cgcccagcat
     5461 gcggccgctc agctggtcga ccagcttgaa cgtcttcagg tcgagcgcat ggcccgtgcc
     5521 cgacagcagg gactccaggt gctcgagcat cggcgggatc acgagctcga agccgtagcc
     5581 gcgggccagg tcgagcagct tgcgccgcag ttcctcgatg cgccgtgctt cggacggcag
     5641 gacatcggcg atgtgctcgg gcaacagcca agcagacgtc atgagaatgc tggtcgggtt
     5701 aaaaccggga ttgtaccgat gtgtccggat cgggcacgcc actatttcgc gccgatgacg
     5761 ggcgcgtgcg ggatcgtcgc aacgccgccg ggcggggcgc cggggcgcgc gccgaccgga
     5821 cctcagccgc ggaacagcac cagaagcccc gcaccgagcg cgaggctcgc gagcccgaac
     5881 aggcggatct ggtggtcggt catcttcacg gcgcgctcga acacgcgccg ccacgtcccg
     5941 gggctcacga agggcaccat gccctcgaac acgagcacca gcgccaccgc gctgaggaac
     6001 aggtcgccca cggccgcgcc gtcagcgctt gcgcgcgggc gcgcctgcgt cggcggcctc
     6061 cgagccgctg ccgcgcatgg ccttgaagaa ctcgctgccg gtgtcgacca cgacgatgtc
     6121 cgacttgcgg aagctcgcgc ggtaggcttg cagcgaccgg tagaacgacg cgaagcgcgg
     6181 gtcgcggccg aaggcctcgc cgtacagctg cgtcgcctgc gcgtcggcct cgcccttgat
     6241 cttctgggcg tcgagcgtgg cgttggcgat gatgaccgcg cgctgcttgt cggcgtcggc
     6301 gcggatctgc tccttgaccg cctcgccttc cgcggtgagc tggttggcca cctgggtgcg
     6361 ctcggagatc atgcgcttgt agaccgcgtc ggtgatctcg gcgacgaagt cgacgcgctt
     6421 gatgcgcacg tcgacgatct cgatgccgaa ggacttcgtg tcctgggcga gccgcgcctg
     6481 cacgttctgc atgatctgct cgcgctcgtc ggagagcacg ccgcgcacgg tgtggcgcgt
     6541 gacctcctcg ttgaacgcgg cctgcacgac cgcgccgagg cggttctcga ggttgtgctg
     6601 ctcgatgccg ttgttgcgga cgtactggcg ggcgtcgctg atgcgccact tgaccatcca
     6661 gtccacgacc aggctctgct tctcggcggt gaagatcgcg cgcggctcgg agctgctgag
     6721 cgtctggatg cgcttgtcga gcatcgagac ggtctgcacc agtggccagt tgaactgcag
     6781 tccgggctcg gtgacgactg accggatctc gccgaaggtc ttgatcacgg cgacgtgctt
     6841 ctggtcgacg acgaacagcg acgacgaggc gaccagcagc accagcagca ggccgatgag
     6901 gggcatgaag atgcggttca cggcgggatc ctcagcgggt atcgcggtcg cgggcgcgcg
     6961 aggcgtcgcg cgcgcgggaa tcgatggcgg acgccgacga cgggtcgacc ggcgtcaccg
     7021 tgaccgaggg cgtcgcgctc gccgaggcgc cgctgccgcc gcccggggac gacgacgacg
     7081 cgccattcgc cgcggacgcc tgcgcgagct tgtccagcgg cagctggatc aagttggtgc
     7141 cgccgtgcgt gtccaccatc accttggcga cgttcgcgta gacctcggtc atggcgtcga
     7201 tgtacatgcg gtcgcgcgtg acggcgggcg ccttctggta ctcggcgagg atggccttga
     7261 agcggtcgga gtcgccctcg gccgcgccga cgatgcgggc cttgtagccc agcgcgtcct
     7321 cgtgcaggcg cgcggcgtcg cccttggccg acgcgatcac ctggttggcg taggcctggc
     7381 cctcgttctt caggcggtcg cggtcgacgc ccgccttgat gacgtcctgg aacgcctggg
     7441 tgacctgttc cggcacggtg acgttctcga tgttgacctt cgcgacgaag acgccggcgt
     7501 tcaggcgatc gagctgcgcc tggatcgact tggccagcgc ctgcggcagc gcctcgcgct
     7561 gctcgtagag gatggagtcg atgcggctgc ggccgacgat ctcgcgcacc gccgactccg
     7621 ccgcctgctg gacggcggtg tccgggtggt cgttgttgaa caggaagtcg cgcgcgtcct
     7681 tcaggctgta ctgcacgttg aagcgcacgt cgacgatgtt ctcgtcctgc gtgagcatcg
     7741 ccgagtcgtg caggccggtg gccgaggaca cgccgttgcc gcccacctcc acggtgcgca
     7801 gctgcgtaaa attgatggtc tcgttgtcct ggatcggcca gggccagcgc cagccccagc
     7861 cggcgccgac ggtgtggctg tagcgcccga aggtggtcac gacggcgcgc tggccctcct
     7921 gcacgatgaa ggagccgctg aacagccaca cgatcacgac gatcagcagg acggccgcga
     7981 tgcccatgcc cgtgctgcgc gggtcggcct ggaagccgcc gccgttgccg ccgcccgcgt
     8041 cgcccttacg gttgcccacg ccgaagaagc gggccagctt ggtcgtgaag tcgcgccaga
     8101 gttcgtcgag gtcgggcggg ccgccgtcct ggccctgggc ccgcaggccg ggcacgaggg
     8161 ccttgcgcag cgccgcgcgc cacgacgcgc ggcgcgaaga gtgggtgttc tcaggcatgt
     8221 tcaagcgtct gatgagggag gcgaggcccg cggccccggg tcgtcgtcgg gcgcattgtc
     8281 gtcggagtcg gccagctcgt cgtcggagtc ggccggctcg tcgtcggggc gtgcgaagcg
     8341 ggcgtcgcgc ggggcgtcct cgcgcggcgc ctcgcggctg gccacgatgg cctgcgcgat
     8401 ggtcgcgcgc agcgcgtcga gaccctcgcc cgtgagcgcg ctcacgaaca cgcgcggcac
     8461 gcgcacgccg tgctcgcctt cgagttcgtc gaccagcgtc tcgggccggc tggccgcgtc
     8521 cagacgatcg agcttgttgt agacgagcac ctgcggcacg tccgccgcgc cgatctcggc
     8581 cagcacgcgc tcgacctcgt cgcgctgctc ctgcagcacc gggctcgccg cgtcgatcac
     8641 gtgcagcagc aggtcggcat ccgtggcctc ctgcagcgtc gcgcggaagg cctccacgag
     8701 cttgtgcggc aggtcgcgga tgaagcccac ggtgtcggac accgataccg actccgccac
     8761 ctcgccgaga tacatctggc gcgtggtggt gtccagcgtc gcgaacagct ggttcgccgc
     8821 atacgcgttg gccttgacca gcgcgttgaa gagggtcgac ttgcccgcgt tcgtgtagcc
     8881 caccagcgag acccggaacg tgccgctgcg ctcgcgcgaa cgacgctgcg tctggcgctg
     8941 gcgcttgacc ttgaccaggc gctccttcac cgacttgatg cggtcgccga tcatgcggcg
     9001 gtccagctcg atctgggtct cgcccgggcc gccgcgcacg cccgccccgc cctgctggcg
     9061 ctccaggtgg ctccagcgcc gcacgaggcg cgtcgacagg tactgcagcc gcgcgagctc
     9121 gacctgcagc ttgccctcgt ggctctgtgc gcgggcggcg aagatctcga ggatcagcgc
     9181 cgtgcggtcg gccacgccca cgccgaggtg ccgttcgagg ttgcgctgct gcgccggcga
     9241 cagcgcctgg tcgaacagca cgccctgcgc gcggtgcgcc aggacgagct ccttgatctc
     9301 gtcggccttg ccgctgccga cgaacagcgc gggatcgggc gccttgcgac gcgcgatcac
     9361 gcgcgcgacg ggcaggtcgc ccgccgactc ggccaggagc gcgagctcgt cgagcgtgtc
     9421 gtcgaaggga gccccgcgcc cgaagtcgac gccgaccagc accgcacgcg gcggcgcggc
     9481 cagcgtcgag ccaccctggc gggcggcccg ctcgtcgttg gagggatccg tgctcaacgc
     9541 gtcgccggcg gaagagcgcc ggacctggga cgggaatggc ttgccggcaa gcccggcgtc
     9601 attccgcggc gtcgcccgga tggaagttca ccgcgcggcc cggcacgacc gtcgagatgg
     9661 cgtgcttgta gaccatctgc gtcaccgtgt tgcgcagcag gacgacgtac tggtcgaacg
     9721 actcgatgtg gccttgcagc ttgatgccgt tgacgaggta gatcgagacg ggaacatgct
     9781 ccttgcgcag caggttcagg aaggggtctt gtaggagttg ccctttgttg ctcacgatat
     9841 tctccgtgtt agaggaagag agaacgttgg aaagcgttca cgttatcaca cgcatgcggc
     9901 gcctcgcgtg tgtacggaca aactttattc ttctttgtcg gtgaacggat tgcggcccga
     9961 gcgcatctcg agcttcagcg gcgtgcccac gagcttgaag tgctcgcgga aacgcccttc
    10021 gaggaagcgc ttgtacgcct ctggcacgtg ctccagcgca ttgccgtgga tcacgatgat
    10081 gggcgggttc atcccaccct ggtgcgcata gcgcatcttg gggcgcgaca tgcccgcgcg
    10141 cggcggttgc tggtgcttca cggcctcctg gagcaggcgc gtgagcaccg gcgtcgtcat
    10201 cttgcggttg gccgacgcgt gggcgtcggc gatgttcttc cacaaggggc cgaggccctg
    10261 ccttttcaag gccgagatgt tgaccgcggg cgcgaatttc aggaacgcga ggcgctggtc
    10321 gatcgagcgc tgcagcatct cgcgctggta gctgtcgacc gcgtcccact tgttgatcgc
    10381 gatgaccacc gcgcggcctg agtcgaggat gtagccggcg atgtgggcgt cctggtcggt
    10441 gacgccctgg gtcgcgtcta gcagcagcac gacgacgttc gcgtcggcga tcgcctgcag
    10501 cgtcttgacg accgagaact tctcgatcgc ctcgaacacg cggcccttgc ggcgcaggcc
    10561 cgcggtgtcg atcagctcga agcggtggcc ctcgcgttcg aagggcaccg tgatcgcgtc
    10621 gcgcgtggtg cccggcatgt cgaacgcgac caggcgctcc tcgccgagcc aggtgttgat
    10681 gagcgtggac ttgccgacgt tgggccggcc cgcgaccgcc aggcggatcg cgcggtcgcc
    10741 cggaccggcg ccgaattcgt cgtgctcggg cgcggcctcc aggccctcga aggcggcgtc
    10801 gagcaggctg cgcacgccct ggccgtgggc cgaggagatc gggtgcagct cgcccatgcc
    10861 gagctcgtgg aactcggcca gcgcgggcga ttcgctcatg ccctcggcct tgttgacggc
    10921 cagcagcacc ttcttctgcg acacccgcag gtatttcgcg atgtcgtggt cctgcgccga
    10981 cagccccccg cgcacgtcga cgacgaagat gacgatgtcc gcctcggcca cggcctggcg
    11041 ggtctggcgc gccatctcct tgacgatccc ggtcgacgag tcgggctcga agccgccggt
    11101 gtcgatgacg atgaactcga tgtcgccgtg gcggccgtcg ccgtagtggc gatcgcgcgt
    11161 gaggccggcg aagtcggcga cgagggcgtc gcgcgtcttc gtgaggcggt tgaagagggt
    11221 cgacttgccg acgttcggtc ggccgaccag ggcgatgacg ggtttcatgc gcgcccctcg
    11281 tccgacgggt acgtcgaccg gtgcccccgg gggactgccg gccggctcgg ggcggcccgg
    11341 cgctcgtccg gcgcgcactt catcatggcg ggactcattc gggacggaac gcgaacacgc
    11401 cgcccgactt cgtcgtcacc gcgatcgtgt tgccggcgcg cacgggcgtc ccgacgatcg
    11461 gggagccgtc ggtctcgagg cgcagcagcg ggtcgccggt cttgcggtcg aggaaatgca
    11521 cccagccctg caggtcgccg aagaccagca ccgtgccgat ggacagcatg ccgctcaggc
    11581 gccggttctg gaacttgtcg ctgagccaga ccgactcgcc gttggagcgc cggcgcgcgc
    11641 tcaggcggtc gctgccgtcg gccgcgaaga cgtagtcctc gtccgcgccc accggctggc
    11701 tgccgcccgc gttgatcgac cacagcgcgg tgacgcgctc ggcgtcgacg cagccgatgc
    11761 cgttctggaa cgcgcggatg cagtagacgt tgccgacgcg cgcggccggg cccacgaggt
    11821 cggcaagacg ctcgacctcg ttggtgccgc gcggcgcggc caccggcacc tcccagcgca
    11881 ccgtgccctt gagcggatcc agggccgtga ggcgggcgcc ctgcccggcc agcagcgtgt
    11941 cctggtacga ggtcaggacg ccggcctggg acagcgtcag cgcatcgccc ggcttcgcat
    12001 acgtccagat gcgcgtgccg tcgagcgcgt cgaaggcgtt gaccgaacga tcgacgccca
    12061 gcgtgaagac gcgctcgccg gcgacgaacg gcgccgtcac gaccgcgctc tgcaggtgct
    12121 ggcgccactt gaccttgccg ccctcgaagg tgacgagttc gccgccacgc gtcacgaccg
    12181 ccgcgaagcg gccgtcggtg ccgacgcccg agctgaggct gccgcccgcg tcggcgcgcc
    12241 agttctcctt gccggagtcg gcgtccaccg agaccacggt gccgttgagc gccgcgatga
    12301 cgaagtcctt
//
LOCUS       DASVLN010000487         2738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_400413, whole genome shotgun sequence.
ACCESSION   DASVLN010000487 DASVLN010000000
VERSION     DASVLN010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400413"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..2733)
                     /locus_tag="VF453_12030"
     CDS             complement(139..2733)
                     /locus_tag="VF453_12030"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX7688431.1"
                     /translation="MRERFRAGKAALFDHFRTGRASTQGAVSLLRALARHVDETLALL
                     WADAGMPASAALLAVGGYGRGELFPYSDVDVLVLLPDPAPAAPADAATPTAAALQSTI
                     ESFITACWDIGLEIGSSVRTLSECLSEARADVTVQTALLEARLVTGQRRLFNAFRRVT
                     GEAMDAKAFLRAKTFEMRQRQVKYEDTPYSLEPNCKESPGGLRDLQVVTWIARAAGLG
                     RTWSEMAANGLLTPFEVKQLQRHEGTLKLIRARLHVIAGRREDRLVFDLQTAVAESFG
                     YAGTTTMRASEKLMQRYYWAAKAVAQLNQVLLLNLEERINGAPEGPMQPINERFLDRS
                     GLLEIVSDDLYLREPHAILETFLLYATTEGLQGLSARTLRALYNVRNQMNAAFRRDPV
                     NRARFVAILQASQRQTHVFRLMNQTSVLGRYLWVFRRIVGRMQHDLFHVYTVDQHILM
                     VLRNVRRFFIPEHAHEYPKCTALASSFERPWVLYVAALFHDVAKGRGGDHSELGGVEV
                     RRFCKDHGIGGDDAALVEFLVRHHLTMSNTAQKADLSDAHVITEFAALVQTPRRLTAL
                     YLLTVADIRGTSPKVWNAWKGKLLEDLFRLTMRALGGDALALDAEIEARKKEARQILA
                     LHSALPGTEAPLWKTLDLSYFARHDPNELAWHARSLWRHLATTVPVVRARPAPHGEGL
                     QVLLFSPDREDLFARICGYFDGAGFSIQDAKIHTTHAGFALDTFQVVNPAHEGEGPAG
                     YRDLIPLVEHQAAAAAGATGPLPTPRASRVSRRVKWFPVVPRVSLRPDERAQRWLLSI
                     SASDRSGLLYAVARVLAQYRINLQLAKVSTLGERVEDTFLVDGAALQNNRLQIQVETE
                     ILEAISAT"
BASE COUNT          382 a          951 c         1009 g          396 t
ORIGIN      
        1 cggtcgacgc gtccgtcgac ggcgccggct acgtcgtgcg cgtggaaggc ggcgcgccgg
       61 cgcaactgcc ggccgcggat cgccaggtgc tcgcgcgcct gttcgtcgag gcggactggg
      121 tcgtcgactg ggcgtgactc aggtcgccga gatcgcctcg aggatctcgg tctcgacctg
      181 gatctgcagc cggttgttct gcagcgccgc gccgtcgacg aggaaggtgt cctcgacgcg
      241 ctcgcccagc gtcgagacct tggcgagctg caggttgatg cggtactgcg ccagcacgcg
      301 cgccacggcg tacagcaggc ccgagcggtc gctcgcgctg atcgacagca gccagcgctg
      361 ggcgcgttcg tccggacgca gcgagacgcg cggcacgacc gggaaccact tcacgcggcg
      421 cgacacgcgg ctggcgcgcg gcgtcggcag cgggccggtc gcgcccgcgg ccgcggcggc
      481 ctggtgctcg acgagcggga tcaggtcgcg gtagcccgcc gggccctcgc cctcgtgggc
      541 cgggttgacg acctggaagg tgtcgagcgc gaagccggcg tgcgtcgtgt ggatcttcgc
      601 gtcctggatc gagaagccgg cgccgtcgaa gtagccgcag atgcgggcga acaggtcctc
      661 gcggtccggc gagaacagca gcacctgcag gccctcgcca tgcggcgccg ggcgggccct
      721 caccaccggc accgtggtcg cgagatggcg ccagagcgag cgcgcatgcc acgcgagctc
      781 gttggggtcg tggcgcgcga agtagctcag gtcgagcgtc ttccacagcg gcgcctcggt
      841 gccgggcagg gcggagtgca gcgcgaggat ctggcgcgcc tccttcttgc gcgcctcgat
      901 ctcggcgtcc agcgcgagcg cgtcgccgcc gagcgcccgc atcgtcaggc ggaacaggtc
      961 ctccagcagc ttgcccttcc acgcgttcca gaccttgggg ctggtgccgc ggatgtcggc
     1021 caccgtcagc aggtacagcg cggtcaggcg gcgcggcgtc tgcaccagcg cggcgaactc
     1081 ggtgatgacg tgggcgtccg agagatcggc cttctgcgcc gtgttgctca tcgtgaggtg
     1141 gtgccgcacg aggaactcga ccagcgccgc gtcgtcgccg ccgatgccgt ggtccttgca
     1201 gaagcgccgc acctcgacgc cgcccagctc cgaatggtcg ccgccccggc ccttggcgac
     1261 gtcatggaac agcgccgcga cgtacagcac ccagggccgc tcgaagctgg aggcgagcgc
     1321 ggtgcatttc gggtactcgt gcgcatgctc ggggatgaag aagcggcgca cgttgcgcaa
     1381 caccatcagg atgtgctggt cgaccgtgta gacatggaac aggtcgtgct gcatgcgccc
     1441 gacgatgcgc cggaacaccc acaggtagcg gcccagcacc gaggtctggt tcatcagccg
     1501 gaagacgtgc gtctggcgct gcgacgcctg caggatggcg acgaagcgcg cgcggttgac
     1561 cgggtcgcgc cggaacgccg cgttcatctg gttgcgcacg ttgtagagcg cccgcagcgt
     1621 gcgcgccgac aggccctgca ggccctcggt ggtcgcgtaa agcaggaagg tctccaggat
     1681 cgcgtgcggc tcgcgcaggt agaggtcgtc gctgacgatc tccagcaggc cgctgcggtc
     1741 caggaagcgc tcgttgatcg gctgcatcgg cccctcgggc gcgccgttga tgcgctcctc
     1801 gaggttgagc agcagcacct ggttcagctg cgccacggcc ttggcggccc agtaatagcg
     1861 ctgcatcagc ttctcggagg cccgcatcgt cgtcgtgccc gcgtagccga agctctcggc
     1921 gaccgcggtc tgcaggtcga agaccaggcg atcctcgcgc cgtccggcga tcacgtgcag
     1981 gcgtgcccgg atcagcttga gcgtgccctc gtggcgctgc agctgcttga cctcgaacgg
     2041 cgtcagcagg ccgttggccg ccatctcgct ccaggtgcgg cccaggcccg ccgcgcgtgc
     2101 gatccaggtc accacctgca ggtcgcgcag gccgcccggg ctctccttgc agttcggctc
     2161 gagcgagtag ggcgtgtcct cgtacttgac ctggcgctgg cgcatctcga aggtcttggc
     2221 gcgcaggaag gccttcgcgt ccatcgcctc gccggtcacg cggcggaagg cgttgaacag
     2281 ccggcgctgg ccggtgacca gccgcgcctc gagcagcgcc gtctgcacgg tcacgtcggc
     2341 acgcgcctcg ctcaggcatt cggacagcgt gcgcaccgac gagccgatct ccagcccgat
     2401 gtcccagcag gcggtgatga aggactcgat cgtcgactgc agcgccgcgg cggtcggggt
     2461 tgccgcgtcc gcgggagccg cgggggccgg atcgggcagc agcaccagca cgtcgacgtc
     2521 ggagtacggg aacagctcgc cgcgcccgta gccgccgacg gccagcagcg ccgcggacgc
     2581 gggcatgccg gcgtcggccc agagcagggc cagcgtctcg tcgacgtggc gggcgagcgc
     2641 gcgcagcagc gacactgcgc cctgcgtcga cgcccgcccg gtgcggaagt ggtcgaacag
     2701 cgccgccttg cccgcgcgga agcgctcgcg cagctcgg
//
LOCUS       DASVLN010000488         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_133065, whole genome shotgun sequence.
ACCESSION   DASVLN010000488 DASVLN010000000
VERSION     DASVLN010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133065"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VF453_12035"
     CDS             <1..473
                     /locus_tag="VF453_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688432.1"
                     /translation="HALAAAAGGLRRLDAPGLDKARLAELVQLERGLADALPQGFLDL
                     EPTGLLRLTRGRPATALPPGMWLVGSHWGPGAVPTELPWDWLFEDAMGADDEGDGLVQ
                     RHSALAGRSATDAGACFDASPVFHTHYLAHEATRVQVRRMLGQALEAGGAGPVR"
     gene            596..796
                     /locus_tag="VF453_12040"
     CDS             596..796
                     /locus_tag="VF453_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688433.1"
                     /translation="MSWADGEIDGLDLVGTTFTFAYGGCKADQFYVSDLAVRTVPQAA
                     VAAPMLSSLELFLMLRRGGRSL"
     gene            complement(869..1771)
                     /locus_tag="VF453_12045"
     CDS             complement(869..1771)
                     /locus_tag="VF453_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688434.1"
                     /translation="MKRRLFPRAPPRAAVVATAAIVLAATSLQLMRSTAMDDPSQHAP
                     RRLQISLGEAVRDYMQVNHLVAQVGHSDIPDKNNYAVAVDVIADTGPIVFGDHWILPD
                     VTIGRQHFELPAGRTLFVDQVAGRINNFSVTPGAQALPLQDTNRMVKPMLDWFLANGS
                     KQEVANSVRLALTDEDADFKRNGEKIYAQLRDTDGNLLNVTVTNLASVPSQPSYIFAP
                     SPPHPMHKPPVYVVRIGFYWAHGDDLSYGDLIYPRRILVNGDKNKILRLRPWVEDPNW
                     TPEKHGMVYLGGTGEGKRWDLPKK"
     gene            complement(1833..2714)
                     /locus_tag="VF453_12050"
     CDS             complement(1833..2714)
                     /locus_tag="VF453_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688435.1"
                     /translation="MVMRSFVLIALAAAAVAASAFHFFDLTAMDDPSKHPPRRLQISL
                     GETVRDFMQVNHLPAEVGHTDTPDKNRYSVAVDVIADTGPVVFWDNWIIPAITIGTQH
                     FELPPGRTLFIDQEAGRIKSFSFTPGAKALPLQETNRLVKPMLDWFLANGWKPEVPNS
                     VTLGLTDQDPDFARSSGKIYAQLMDDSRNQLSVTVTNLASVPSQPSYIMAPSPARPKH
                     DPPVYVVRVGFYWAHRNDLSYGDLIFPRRIFVNGDKNNFLRLRPWVEDPDWTPEKHGM
                     VYLGGTGEGKRWGLPKK"
     gene            complement(2708..3499)
                     /locus_tag="VF453_12055"
     CDS             complement(2708..3499)
                     /locus_tag="VF453_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2515 family protein"
                     /protein_id="HEX7688436.1"
                     /translation="MTEPTDVSSQAANAVQQSGNGDAAGVCVTCCKAKVQDIDDQTRK
                     IAAIDDPIERNKAITKAYTDVAAQDPQDRWVKLASIVSAQGGCAMKQVAGVRSYLSWK
                     QPLDDATGTSFTNNMYHALGDANKAIFTDIYPVAAYKAKYGWTSLKECYASEGKTLPN
                     ELKDAFDKMDAGDLSGASNSIAKFEQTQVVQPVYDKYSGTFAEMKVASAIQKPFTGKN
                     LYDIPVSTTCGDPDTIPFSGNISNGDDRVVYYEALMRKLAQQQGW"
     gene            complement(3496..3852)
                     /locus_tag="VF453_12060"
     CDS             complement(3496..3852)
                     /locus_tag="VF453_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688437.1"
                     /translation="MPIMIKPAQYAALEAQQLRTFAAEVARTLSAIPSNHATPSTPGG
                     PVEFAHEQVQRASRLGLKRRNEMMAWALCAWVHGTDFDHRMPEIRQIVAEPNYDRSMM
                     LTVVAMHGLRGEGQAA"
     gene            complement(4016..>4232)
                     /locus_tag="VF453_12065"
     CDS             complement(4016..>4232)
                     /locus_tag="VF453_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688438.1"
                     /translation="LYAGAVLLLARPGRGRALFFIAAVGLGLSIPAWGVDATWTWPVA
                     AGAMLALAGAWYARPEVHVPEDAEAQP"
BASE COUNT          658 a         1355 c         1437 g          782 t
ORIGIN      
        1 ggcacgccct ggccgcggcg gcgggtggcc tgcgccgcct cgacgcgccc ggcctcgaca
       61 aggcccgcct cgccgagctc gtgcaactcg aacgcggcct ggccgatgcg ctgccccagg
      121 gcttcctcga cctcgagccc accggcctcc tccgcctgac ccgcggccgc cccgcgaccg
      181 cgctgccgcc cggcatgtgg ctcgtcggct cccactgggg cccgggcgcc gtcccgaccg
      241 agctcccctg ggactggctc ttcgaggacg ccatgggcgc cgacgacgag ggcgacggcc
      301 tcgtccaacg ccactccgcc ctggccggcc gaagcgccac cgacgccggc gcctgcttcg
      361 acgcctcgcc ggtcttccac acgcactacc tcgcgcacga ggcgacgcgg gtgcaggtga
      421 ggaggatgct cgggcaggcg ttggaggcag gcggcgccgg gcccgtgcgc tgaagcgggc
      481 acggacgctc agtccttctg ccgacgcatg ccgttgcggt gccagtgtgc cgcgaaggtc
      541 gccgtgatcg cgcctgccat ccggaagcgg gcgcgccgcc tcggaacccc tgcttatgtc
      601 ctgggccgac ggggagatcg atggcctcga cttggttggg acgactttca ccttcgctta
      661 cggcggatgc aaggccgacc agttctacgt gagcgatctc gcggtgagga ctgtgcccca
      721 agctgcggtg gcagcgccga tgctctcaag cctggagctc ttcctcatgc tgcgtcgcgg
      781 cgggcgaagc ctctgaccct gggcagcccg tcggcgacgg cgggcgccct cgggcggcca
      841 ttccggggag acagactccc gggcttgcct acttcttcgg cagatcccag cgcttgccct
      901 cgccggttcc gccgagatag accatgccgt gcttctctgg cgtccagttc ggatcttcga
      961 cccacggccg cagccgcagg atcttgttct tgtcgccgtt gacgaggatg cgtcgcgggt
     1021 agattaggtc gccgtaggag aggtcgtcgc cgtgggccca gtaaaagccg atccgcacca
     1081 cgtagacggg tggcttgtgc atgggatgcg ggggcgacgg ggcgaagata tagctcggct
     1141 ggctcggcac cgacgccaag tttgtcactg tcacgttcag cagattgcca tcggtgtccc
     1201 gcaattgcgc gtagatcttt tctccgttgc gcttgaaatc cgcatcctcg tctgtcagcg
     1261 ccaaccgcac gctgttggcg acttcctgct tcgagccgtt cgccaggaac caatccagca
     1321 tcggcttcac catccgattc gtatcctgta gcggtaacgc ctgagcgccg ggcgtgacgg
     1381 agaaattgtt gatccggccg gcgacctggt cgacgaagag agtgcggccc gccggtagct
     1441 cgaagtgctg ccttccgatg gtgacatccg gaagtatcca gtgatccccg aagacgatcg
     1501 gtccggtgtc ggcaatgacg tctacggcta cggcgtagtt gttcttgtcc ggaatgtcgc
     1561 tgtggccgac ctgggcgacc aagtggttga cttgcatgta atcgcggacg gcttccccca
     1621 aggaaatctg gaggcggcgc ggggcatgct ggctgggatc gtccatggca gtggagcgca
     1681 tgagctgcaa actggttgct gcgaggacga tggccgcggt cgcgacgacc gcagcccgcg
     1741 gcggggccct gggaaagagg cgtcgcttca ctcggatgga tgtggacgtg aaaggactcg
     1801 tcgagcgcgc atccggaggc gccgtccagg ggctattttt taggaaggcc ccaacgcttg
     1861 ccctcgcccg tccccccgag atagaccatc ccgtgcttct ccggcgtcca gtcgggatcc
     1921 tcgacccacg gccgcaggcg aaggaagttg ttcttgtcgc cgttcacgaa gatgcggcgt
     1981 ggaaagatca ggtcgccgta ggacagatca ttgcggtgtg cccagtagaa gcccactcgg
     2041 acgacgtaga caggaggatc gtgtttgggc cgcgcaggcg atggcgccat gatgtagctc
     2101 ggctggctcg gcacggaggc caggttggtg actgtgacgc tcagctggtt gcgcgagtcg
     2161 tccatgagct gggcatagat tttcccgctg ctgcgtgcga agtctggatc ctggtccgtc
     2221 agccccaggg tcacgctgtt ggggacttcg ggcttccagc cgttcgccaa gaaccagtcc
     2281 agcatgggct tgacgagccg gtttgtctcc tgcagtggaa gcgccttcgc tcccggggtg
     2341 aacgagaagc tcttgatccg gcccgcttcc tgatcgatga acagggtgcg gcccggcggc
     2401 agttcgaagt gctgcgtccc gatcgtgatg gcaggaatga tccagttgtc ccagaaaacg
     2461 accgggcctg tatcggcgat gacgtccacg gcgacggagt acctgttctt gtctggcgtg
     2521 tcggtatgtc cgacttcggc gggcaagtgg ttgacctgca tgaaatcgcg gacggtttcc
     2581 ccgagggaaa tctggaggcg gcgcggggga tgcttgctgg gatcgtccat agcggtgaga
     2641 tcgaagaagt ggaaggccga ggcggctacg gcggcggccg ccagggcgat gagaacgaag
     2701 cttcgcatca ccatccctgt tgctgcgcta gcttgcgcat cagcgcttcg tagtagacga
     2761 cgcggtcgtc gccgttcgaa atatttccag agaagggaat ggtgtcggga tcgccgcagg
     2821 tcgtcgacac cgggatgtcg tagaggttct ttcccgtaaa aggcttctga atcgccgaag
     2881 ccaccttcat ttcagcgaaa gttccggaat atttgtcata gacgggctgc accacttgag
     2941 tctgttcgaa cttggcgatg ctgttggacg cgccgctaag gtcgcctgca tccatcttat
     3001 cgaacgcgtc cttcagctcg ttcggcagcg tctttccctc cgacgcataa cactccttga
     3061 gactcgtcca cccatacttc gccttgtacg ccgccaccgg atagatgtcc gtgaagatcg
     3121 ccttgttggc gtcgcccagc gcgtgataca tgttgttcgt gaacgacgtg cccgtcgcgt
     3181 cgtcgagcgg ctgcttccac gagaggtagg agcgcacgcc agccacctgc ttcatcgcgc
     3241 agccgccctg cgccgagacg atggaagcca gcttgaccca gcggtcttgc ggatcctgtg
     3301 cggcgacatc ggtgtaggcc ttcgtgatgg ccttgttgcg ttcgatcggg tcgtcgatcg
     3361 ccgcgatctt gcgcgtctgg tcgtcgatgt cctggacctt ggccttgcag caggtcacgc
     3421 atacgccggc ggcgtcgccg ttgccggact gctggacggc gttggcggcc tgcgaggaga
     3481 cgtcggtcgg ttcagtcatg cggcttgccc ctcgccgcgc aggccgtgca tcgcgaccac
     3541 cgtcagcatc atggagcggt cgtagttcgg ctccgccacg atctggcgga tctcgggcat
     3601 ccggtggtcg aagtcggtac cgtgcaccca ggcgcacagg gcccatgcca tcatctcgtt
     3661 gcggcgcttc aggccgagcc ggctcgcccg ctggacttgc tcgtgggcga attcgacggg
     3721 cccgcccggc gtagacggcg tagcgtgatt cgagggaatg gcgctcaagg tgcgggcgac
     3781 ctcggcggcg aaggtgcgca actgctgcgc ttccagcgcc gcatattgcg ccggcttgat
     3841 catgatcggc atctacggga gacctgtcag gcgtcgaccg aggaatctaa cagccgctcg
     3901 cagcgtcgcg gttccccccg atgggggttg cctcggcgcc gaacagagct gaccggggcg
     3961 ttccgtcgcg ccacaagatg cctcggcgcc ttcgggtcag ctcgccggac gaccctcagg
     4021 gctgcgcctc ggcgtcctcg ggcacgtgca cctcggggcg cgcataccag gcgccggcga
     4081 gcgccagcat cgcgcccgcg gccacgggcc aggtccaggt ggcgtcgacg ccccaggcgg
     4141 ggatcgacag gccgaggccg acggcggcga tgaagaagag cgcgcggccg cggccggggc
     4201 gggcgagcag gagcacggcg ccggcataca gc
//
LOCUS       DASVLN010000489         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_239502, whole genome shotgun sequence.
ACCESSION   DASVLN010000489 DASVLN010000000
VERSION     DASVLN010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239502"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VF453_12070"
     CDS             complement(<1..658)
                     /locus_tag="VF453_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7688439.1"
                     /translation="MNPDDPHYERRRWWALAVLCLGVLMIVLDTTIVNVALPSIRADL
                     RFDDASLSWVVNAYMLTFGGSLLLGGRLGDLYGHRRLFIIGLLGFSGASFACGLAPTQ
                     GVLVAARAVQGVAGAVVSAISLSLIMSLFSSEADRAKAMGVYGFVCSAGGSIGVLLGG
                     VLTGLLSWHWIFLVNVPIGLAVAALCGRMLPAGAHAGGERRLDVPGALLVTASLMLAI
                     F"
     gene            complement(748..1308)
                     /locus_tag="VF453_12075"
     CDS             complement(748..1308)
                     /locus_tag="VF453_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEX7688440.1"
                     /translation="MRTDIIERIEHWFALHGAQSYEGARRESVSALQHALQCAQLAED
                     ARADAALVAAALLHDIGHFVALAELPGHEDVDDLHELRGVAVLVPHFPAAVYEPVRRH
                     VAAKRWLTAVEPAYLQALSPASMHSLAQQGGPMDAAEIAAFEASPFADDAVRLRRWDD
                     LAKVPDRATPSLDYYLALLDEVRLRA"
     gene            complement(1320..2027)
                     /gene="phnE"
                     /locus_tag="VF453_12080"
     CDS             complement(1320..2027)
                     /gene="phnE"
                     /locus_tag="VF453_12080"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX7688441.1"
                     /translation="MLAASWKGAEMNPLALIAGSGNIATYARDYFPPAFGDWREYLSE
                     LVVTLEISLWGTALAIVCAMPLALLAAANIAPWWIRHPVRRLLDTFRAINDMVYALLF
                     VTAVGLGPFSGVLALWVSTVGILGKLFAEAVETIDPSPVEALRATGAHPLNVVLYGVL
                     PQVTPLWVSYALYRFESNVRSASVVGMVGAGGIGMVLWDVVRGFEYAKTSAVLIMLVV
                     SVMLIDMASAWLRKRLV"
BASE COUNT          340 a          743 c          703 g          243 t
ORIGIN      
        1 cgaagatcgc gagcatcagc gacgcggtga ccagcagcgc acccggcacg tcgagccggc
       61 gctcgccgcc cgcgtgcgcg ccggcgggca gcatgcgtcc gcacagcgcc gcgacggcca
      121 ggccgatcgg cacgttgacg aggaagatcc agtgccagct gagcaggccc gtcagcacgc
      181 cgcccagcag cacgccgatg ctgccaccgg ccgagcagac gaagccgtag acgcccatgg
      241 ccttcgcccg gtcggcctcg ctcgagaaca ggctcatgat cagcgacagc gagatcgccg
      301 acaccacggc gccggcgacg ccctgcaccg cgcgcgcggc gaccagcacc ccctgcgtcg
      361 gcgcgaggcc gcacgcgaac gaggcgccgc tgaaacccag caggccgatg atgaacaggc
      421 gccggtggcc gtagaggtct cccagccgcc cgcccagcag caggctgcct ccgaaggtca
      481 gcatgtaggc gttgacgacc cacgaaaggc tcgcgtcgtc gaagcggagg tcggcgcgga
      541 tcgagggcag cgccacgttc acgatcgtcg tgtccagcac gatcatcagc acgcccaggc
      601 acagcaccgc cagcgcccac cagcgccggc gttcgtagtg gggatcgtcg gggttcacgc
      661 ggactcctcg gaggacggac gatggcgcgc cattgtccac gccttcgagg ccccgccgcc
      721 gtccgggatc gcgtagtccg cgagcgctca ggcgcgcagg cgcacctcgt ccagcagcgc
      781 gaggtagtag tccagcgaag gcgtggcgcg atccggcacc ttggcgaggt cgtcccagcg
      841 gcgcaggcgc accgcgtcgt cggcgaatgg cgaggcctcg aacgcggcga tctcggccgc
      901 gtccatcggc ccgccctgct gcgccaggga gtgcatcgag gccggcgaca gcgcctgcag
      961 gtacgcgggc tccactgcgg tgagccagcg cttggccgcg acgtggcggc gcaccggctc
     1021 gtagaccgcg gcggggaagt gcggcaccag cacggcgact ccgcgcagct cgtgcaggtc
     1081 gtcgacgtcc tcgtggccgg gcagttcggc cagcgcgacg aagtggccga tgtcgtgcag
     1141 cagcgcggcg gccacgagcg ccgcatccgc ccgcgcatcc tcggccagct gcgcgcactg
     1201 cagcgcgtgc tgcagggcgc tcaccgactc gcgcctggcg ccctcgtacg attgcgcgcc
     1261 gtgcagcgcg aaccagtgct cgatgcgttc gatgatgtcg gtccgcatgg ttcttcccgt
     1321 cacacgagcc gcttgcgcag ccaggcggag gccatgtcga tgagcatcac cgacacgacc
     1381 agcatgatca gcacggcgct cgtcttggcg tactcgaagc cgcgcaccac gtcccacagc
     1441 accatgccga tgccgcccgc gcccaccatg ccgacgaccg aggccgagcg cacgttggac
     1501 tcgaagcggt acagcgcata cgacacccac agcggcgtga cctgcggcag cacgccgtac
     1561 agcaccacgt tgagcggatg ggcgccggtc gcgcgcagcg cttcgaccgg gctggggtcg
     1621 atcgtctcga ccgcctcggc gaacagcttg cccaggatgc cgaccgtgct cacccagagc
     1681 gcgagcacgc ccgagaacgg cccgaggccg accgcggtga cgaacagcag cgcgtacacc
     1741 atgtcgttga tcgcgcggaa ggtgtcgagc agccgtcgta ccgggtgacg tatccaccac
     1801 ggcgcgatgt tggccgccgc cagcagcgcg agcggcatcg cgcagacgat cgcgagcgcg
     1861 gtgccccaca gcgagatctc gagcgtgacc accagctccg agaggtactc gcgccagtcg
     1921 ccgaacgcgg gcgggaagta atcgcgcgca taggtcgcga tgttgccgct gccggcgatc
     1981 agcgcgagcg gattcatctc cgcgcccttc cagctggcgg ccagcagcg
//
LOCUS       DASVLN010000490         2668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_79677, whole genome shotgun sequence.
ACCESSION   DASVLN010000490 DASVLN010000000
VERSION     DASVLN010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79677"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..603)
                     /locus_tag="VF453_12085"
     CDS             complement(118..603)
                     /locus_tag="VF453_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7688442.1"
                     /translation="MKLTPIQKRAARKARHGNQLDMNLVSLIDVFTILIFFLLSNSGV
                     ETLPPTGTVHLPESTAQKTPRETLVVAVNGTEITVDGRKVADVAPLATAADDLIPGLK
                     AELDQLAARPVARAEDAAKPKAVTIMGDKTIPYQLLRKVMYTCAAADYTEVSFAVNRK
                     G"
     gene            complement(620..1114)
                     /locus_tag="VF453_12090"
     CDS             complement(620..1114)
                     /locus_tag="VF453_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7688443.1"
                     /translation="MPIRRLRKHPEALEVSAFINLIVVLVPFLLSTAVFSRISIVDMT
                     LPARSSGLEHVEPGHLLLEVVVRHDKIEVNDKNGGQLYQPIPVGADGPDLGLLSADLL
                     DLKKRFPDTTAASLLPEPNIPYDEMIQVMDTMRTTKSANGGTEVVDVDLFPAISIGDA
                     PVRR"
     gene            complement(1116..1766)
                     /locus_tag="VF453_12095"
     CDS             complement(1116..1766)
                     /locus_tag="VF453_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7688444.1"
                     /translation="MSTLDSAIKFFQDCGLFIYPSLLIMATGVSIAIERFVFLQRARV
                     ANRRDWATVLPLLQSGQWKEVMSKTAASDSAIGKIVHNGLTRLQSTRRRDDIDAAMEE
                     GMMEIVPRLEKRTHYIAVFANVITLVGLLGTITGLIHAFTAVAQVNPSEKASLLSAAI
                     SIAMNNTAFALMVAIPFLLIHAFLQARTSDIVDGLEAAKISFLNLVQRLAPEHKAA"
     gene            complement(1819..2160)
                     /locus_tag="VF453_12100"
     CDS             complement(1819..2160)
                     /locus_tag="VF453_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688445.1"
                     /translation="MIRSATLAAIAALAAAPALAQDRADLDATRIIGNRELPQALYIV
                     PWKRPLPGDLVGRPPASVIDEALAPIDRDVFRREVRYDSIERARMAAAAPPRPAAAPA
                     PATAASASSAH"
     gene            complement(2157..>2668)
                     /locus_tag="VF453_12105"
     CDS             complement(2157..>2668)
                     /locus_tag="VF453_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7688446.1"
                     /translation="AFDDALAALKAGRDAEARTRLQAIAKSDPELAGPHANLGLIARH
                     AGRLDEAVAELERATTLDPRQPVMWNQLGITYRQDGQFAKARAAYEHAIALDPDYATA
                     IVNLGVLEDLYLGEPAQALALYTRYLALTPAGDPLVAKWVADLRNRLPKPAAAPASSA
                     ASAAGKESS"
BASE COUNT          374 a          857 c         1014 g          423 t
ORIGIN      
        1 aacgcgagca ccagccccgt gatgatcgcg aacaggcgac gaaagcgttg ctcgtcgtcg
       61 tgcgcgagcg cccacggcag caccgagctc ttgaacgaga gggtggcggt cacggcgtca
      121 tcccttccgg ttcacggcga acgacacctc ggtgtagtcc gccgccgcgc aggtgtacat
      181 gaccttgcgc agcagttggt aggggatcgt cttgtcgccc atgatggtca cggccttggg
      241 cttggcggcg tcctccgcgc gcgcgaccgg gcgcgcggcg agctggtcga gctcggcctt
      301 caggcccggg atcaggtcgt cggcggcggt ggccaggggc gccacgtccg ccaccttgcg
      361 gccgtcgacc gtgatctcgg tgccgttgac ggcgacgacc agcgtctccc tgggcgtctt
      421 ctgagcggtc gattcgggca ggtgcacggt gccggtgggc ggcagcgtct ccacgccgga
      481 attcgatagc aggaagaaga tcaggatcgt gaagacgtcg atcagcgaca cgaggttcat
      541 gtcgagctgg ttgccgtgcc gggccttgcg cgcggcgcgc ttctggatcg gggtcagctt
      601 catcgcgtgg ccctttcctt cagcggcgca ccggcgcgtc gccgatcgag atcgccggga
      661 acaggtcgac gtcgacgacc tcggtgccgc cgttggccga cttggtcgtg cgcatcgtgt
      721 ccatcacctg gatcatctcg tcgtacggga tgttgggctc gggcagcagc gaggcggccg
      781 tggtgtcggg gaagcgcttc ttcaggtcga ggaggtcggc gctgagcagc ccgagatccg
      841 ggccgtcggc cccgaccggg atcggctggt acagctgccc gccgttcttg tcgttcacct
      901 cgatcttgtc gtggcgcacg acgacctcca gcagcaggtg ccccggctcg acatgctcca
      961 gcccggagct cctggccggc agcgtcatgt ccacgatgga gatgcgcgag aacacggccg
     1021 tcgagagcag gaagggcacc agcacgacga tcaggttgat gaatgcgctg acctccaggg
     1081 cctctggatg cttgcgcagg cgtctgatcg gcatgtcagg ccgccttgtg ctcgggcgcc
     1141 aggcgctgga cgaggttcag gaagctgatc ttcgcggcct cgaggccgtc gacgatgtcc
     1201 gaggtgcgcg cctgcaggaa cgcgtggatc agcaggaacg ggatcgcgac catcagcgcg
     1261 aacgccgtgt tgttcatcgc gatcgagatc gccgccgaca gcagcgaggc cttctccgac
     1321 gggttgacct gcgcgaccgc cgtgaacgcg tggatgaggc ccgtgatcgt gcccagcagg
     1381 cccaccagcg tgatgacgtt ggcgaacacg gcgatgtagt gggtgcgctt ctccaggcgc
     1441 ggcacgatct ccatcatccc ttcctccatc gcggcgtcga tgtcgtcgcg gcgacgcgtg
     1501 gactgcaggc gcgtgaggcc gttgtggacg atcttgccga tcgccgagtc cgacgccgcc
     1561 gtcttgctca tgacctcctt ccactggccg ctctgcagca gcggcagcac cgtcgcccag
     1621 tcgcggcggt tggcgacgcg cgcgcgctgc aggaagacga agcgctcgat ggcgatcgac
     1681 acgcccgtgg ccatgatcag caggctgggg tagatgaaca ggccgcagtc ctggaagaac
     1741 ttgatggcgg agtcgagggt gctcatggaa atctccgggg aacgctggtg tcgggagtga
     1801 aacgggaagc cgcgcgggtc agtgcgcgga ggaggcggat gcggccgtgg cgggagcggg
     1861 cgcggccgcg ggcctgggcg gcgcggcggc ggccatgcgg gcgcgctcga tcgagtcgta
     1921 gcgcacctcg cggcggaaga cgtcgcggtc gatcggcgcg agcgcctcgt cgatcacgct
     1981 ggccggcggg cggccgacga ggtcgccggg cagcggccgc ttccacggca cgatgtacag
     2041 cgcctgcggc agctcgcggt tgccgatgat gcgggtcgcg tcgaggtcgg cgcgatcctg
     2101 ggcgagggcc ggcgcggcgg cgagcgccgc gatcgcggcg agggtggcgg atcggatcat
     2161 gaggactcct tgccggcggc cgaggccgcg gaggacgccg gcgctgcggc gggcttgggc
     2221 aggcggttcc tgaggtcggc cacccacttg gccaccagcg ggtcgcccgc cggcgtcagc
     2281 gccaggtagc gcgtgtacag cgcgagcgcc tgcgcgggct cgccgaggta cagatcctcg
     2341 agcacgccca ggttgacgat cgcggtggcg tagtcgggat cgagcgcgat cgcgtgctcg
     2401 taggccgcgc gcgccttggc gaactggccg tcctggcggt aggtgatgcc cagctggttc
     2461 cacatgacgg gctggcgcgg atccagcgtc gtcgcgcgct ccagctcggc gacggcctcg
     2521 tccaggcgcc cggcgtggcg cgcgatcagg cccaggttgg cgtgcgggcc ggcgagctcg
     2581 gggtcggact tggcgatcgc ctgcaggcgc gtgcgcgcct cggcgtcgcg gccggccttc
     2641 agcgcggcga gcgcgtcgtc gaacgcgc
//
LOCUS       DASVLN010000491         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_456128, whole genome shotgun sequence.
ACCESSION   DASVLN010000491 DASVLN010000000
VERSION     DASVLN010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456128"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..283)
                     /locus_tag="VF453_12110"
     CDS             complement(74..283)
                     /locus_tag="VF453_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX7688447.1"
                     /translation="MKSWQLVRAFAGAFILVSLLLGVPASPLYLSTWWLGLAAFVGAN
                     LLQSAFTQWCPLELILRRLGVRGGC"
     gene            complement(280..3543)
                     /locus_tag="VF453_12115"
     CDS             complement(280..3543)
                     /locus_tag="VF453_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7688448.1"
                     /translation="MSTRPGISGRIAARFQAAQITPLLALVALLLGLFALVVTPREEE
                     PQINVTMANVLIPFPGASAKDVEAMVATPAEQVMSQIAGVEHVTSASRPGVAMVTVQF
                     KVGVPRTEALVRLYDTVHSHADWLPPGLGVGPPIVKPKGIDDVPIVALTLFSRDPATG
                     PFALERVAHSMEADLKRVPGTREVTTIGGPGRAVMVEIDPQRLAGAGVTVADLRQALQ
                     SANMSLPLGELLGADRSVALEAGPFLRDARDVGDLVVGARGGKPVFVKDVAVVRDGAP
                     PPQRYAWHGVAGPKGGEFPAVTIAVTKKPGENAIAVADAVMQRVQLLRNTVIPQGVEV
                     AETRNYGATANDKADKLIHKLLFATASVVALVFFALGRREAAIVGSAVVLTLTATLFA
                     SWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHQLLHPGKRLAQLIPGAVDEVG
                     GPTILATLTVIAALLPMAFVSGLMGPYMSPIPINASMGMLLSLAIAFVVTPWLARLWM
                     KEHPARAQSREARGPTGLAARIGPFFERVFRPLLDERRGRRNRALLGGGVLALIALAL
                     ALPALGLVVLKMLPFDNKSEFQVVVDMPAGTPLERTAAVLHELGSYLARQPEVVDYQA
                     YAGTASPINFNGLVRQYDLRSGGDVGDLQVNLVDKSRRREQSHVIAMRLRPGLQAIGR
                     RFDANVKVVEVPPGPPVLSPIVAEIYGPDAQGRRAVAKAVRAVFEKTPGIVDADDSGI
                     AAAPRTQLLVDRRKAALLGVPQAAIVSTLRAGLAGEAATYLHDESKYPAPALIQLPPE
                     RQGDLDALLQLRVRGSAGELVPIRELVTASDTLREQVIHHKDLLPVDYVVADMAGALD
                     SPLYGVFRMRPALRDIAPPGGGQLQEYFIHPPQDAWRGYALKWDGEWQITYETFRDMG
                     AAYAVGLVLIYLLVVAQFGSYLTPLIIMAPIPLTIVGVMPGHALLHAQFTATSMIGMI
                     ALAGIIVRNSILLVDFIRLQVDQGVPLADAVVRSAATRAQPIVLTGLAAMLGAFFILD
                     DPIFNGLAISLIFGIFVSTVLTLVVIPLLYFVASRRPSATPPPGGPSPAASTEGACT"
     gene            complement(3565..>3969)
                     /locus_tag="VF453_12120"
     CDS             complement(3565..>3969)
                     /locus_tag="VF453_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7688449.1"
                     /translation="ETGRAQVELGVAPGARRVTPTRTQWLPEVDPATHTQEVRLDLPT
                     GLQGVRPGAFARVWIAAEASSERTSLLVPARAVVRRAELAAVYVVNGQGVPVLRQVRL
                     GPARGDRVEVLSGVSAGESIALDPQAAAAAGR"
BASE COUNT          598 a         1367 c         1479 g          525 t
ORIGIN      
        1 ggcgcgacgg cgagcgcgag cgccgccggc agcgcggcca gcgcgggggt caggatgcgc
       61 ggacgcatcg ggctcagcag ccgccgcgca cgcccaggcg ccgcaggatg agttccagcg
      121 ggcaccattg cgtgaacgcg ctctgcagca ggttggcgcc gacgaaggcc gcgagcccga
      181 gccaccacgt gctgaggtag agcgggctgg cgggtacccc gagcagcagc gacacgagga
      241 tgaacgcgcc cgcgaaggcg cgtacgagtt gccaggactt catgtgcaag ctccttcggt
      301 ggatgcggcg ggggaggggc cgccgggcgg cggcgtggcg gacgggcgcc gcgaggcgac
      361 gaagtacagc agcggaatca ccacgagggt gagcaccgtg ctgacgaaga tgccgaagat
      421 cagcgagatc gcgagcccgt tgaagatcgg gtcgtcgagg atgaagaagg cgccgagcat
      481 cgcggccagg ccggtcagca cgatcggctg cgcccgggtg gcggccgagc gcaccacggc
      541 gtcggccagc ggcacgcctt ggtcgacctg gaggcggatg aagtccacca gcaggatcga
      601 attgcgcacg atgatgccgg ccagcgcgat catgccgatc atgctggtgg cggtgaactg
      661 cgcgtggagc agcgcatggc cgggcatcac gcccacgatc gtgagcggaa tgggcgccat
      721 gatgatcagc ggcgtgaggt acgagccgaa ctgggcgacg accagcaggt agatcagcac
      781 caggcccacc gcgtaggccg cgcccatgtc gcggaacgtc tcgtaggtga tctgccactc
      841 gccgtcccac ttcagcgcgt agccgcgcca ggcgtcctgc ggcgggtgga tgaagtattc
      901 ctgcaactgg ccgccgccgg ggggcgcgat gtcgcgcaat gccggacgca tgcggaacac
      961 cccgtacagc gggctgtcga gcgcgccggc catgtcggcc accacgtagt ccaccggcag
     1021 caggtccttg tgatggatca cctgctcgcg cagcgtgtcg ctcgccgtga ccagctcccg
     1081 gatcggcacg agctcgccgg cgctgccgcg cacgcgcagc tgcagcaggg cgtcgaggtc
     1141 gccctggcgc tcgggcggca gctggatcaa ggccggtgcg gggtacttgc tttcgtcgtg
     1201 caggtaggtg gcggcctcgc cggccaggcc cgcgcgcagc gtgctcacga tcgcagcctg
     1261 cggcacgccg agcagcgcgg ccttgcggcg atcgacgagc agctgcgtgc gcggcgcggc
     1321 cgcgatgccg ctgtcgtcgg cgtcgacgat gccgggcgtc ttctcgaaca cggcgcgcac
     1381 ggccttggcg accgcgcgcc ggccttgcgc gtcggggccg tagatctcgg cgacgatcgg
     1441 cgacagcacc ggcggtccgg gcggcacttc caccaccttc acgttggcgt cgaagcgccg
     1501 gccgatcgcc tgcaggccgg gccgcagccg catggcgatg acgtggctct gctcgcggcg
     1561 gcgcgacttg tccaccaggt tcacctggag gtcgcccacg tcgccgccgc tgcgcaggtc
     1621 gtactggcgc acgaggccgt tgaagttgat cggcgaggcg gtgccggcat aggcctggta
     1681 gtcgacgacc tcgggctgcc gggcgaggta gcttcccagc tcgtggagca cggccgcggt
     1741 tcgctcgagc ggtgtgccgg caggcatgtc gaccaccacc tggaactcgg acttgttgtc
     1801 gaagggcagc atcttcagca cgaccaggcc gagcgcgggc agcgcgagcg cgagggcgat
     1861 cagcgcgagc acgccgccgc ccagaagcgc acgattgcgg cgtccgcgac gctcgtccag
     1921 gagcgggcgg aagacgcgct cgaagaaggg gccgatcctg gcggcgaggc cggtcggccc
     1981 gcgcgcctcg cgggactgcg cgcgcgcagg gtgctccttc atccacagcc gggcgagcca
     2041 cggcgtgacg acgaacgcga tcgccagcga cagcagcatc cccatgctcg cgttgatcgg
     2101 gatcgggctc atgtacgggc ccatcagtcc gctgacgaag gccatgggca ggagcgcggc
     2161 aatgacggtg agcgtcgcca ggatggtcgg cccgccgacc tcgtcgaccg cgccggggat
     2221 cagctgcgcc aggcgcttgc cgggatggag cagctggtgc cggtggatgt tctcgacgac
     2281 gacgatcgca tcgtcgacca ggatgccgat cgagaagatc agcgcgaaca gcgacacccg
     2341 gttgagcgtg aagccccagg cccaggaggc gaacagcgtc gcggtgagcg tgagtaccac
     2401 ggcgctgccg acgatcgccg cctcgcgccg gccgagcgcg aagaacacca gcgcgaccac
     2461 cgatgcggtc gcgaacagca gcttgtggat cagcttgtcg gccttgtcgt tggccgtggc
     2521 gccgtagttg cgcgtctcgg cgacctcaac gccttggggg atcaccgtgt tgcgcagcag
     2581 ctgcacccgc tgcatcaccg catcggccac ggcgatggcg ttctcgccgg gcttcttggt
     2641 gacggcgatg gtcacggcgg gaaactcgcc gcccttcggg ccggcgacgc cgtgccacgc
     2701 gtagcgctgc ggcggcggcg cgccgtcacg gaccaccgcc acgtctttca cgaacacggg
     2761 cttgccgccg cgcgctccga cgacgaggtc gcccacgtcg cgcgcgtcgc gcaggaaggg
     2821 gccggcctcc agcgccacgc tgcggtcggc gccgagcagc tcgcccagcg gcaggctcat
     2881 gttcgccgat tgcagcgcct gccgcaggtc cgccaccgtc actcccgcgc cggcgaggcg
     2941 ctgcgggtcg atttccacca tcaccgcgcg cccggggccg ccgatggtcg tgacctcgcg
     3001 cgtgccggga acgcgcttga gatcggcttc catgctgtgg gccacgcgct ccagggcgaa
     3061 ggggcccgtg gccgggtccc ggctgaagag cgtgagcgcg acgatgggca cgtcgtcgat
     3121 gcccttgggc ttgacgatcg gcggccccac gcccagtccc gggggcagcc agtcggcatg
     3181 cgaatgcacc gtgtcgtaca ggcgcaccaa cgcctcggtg cgcggcacgc ccaccttgaa
     3241 ttgcacggtc accatcgcga cgccgggacg cgatgccgac gtcacgtgtt ccactccagc
     3301 gatctgcgac atcacctgct cggccggggt ggcgaccatc gcttcgacgt ccttggcgga
     3361 ggcgccggga aaggggatga gcacgttggc catcgtcacg ttgatctgcg gctcttcctc
     3421 gcgcggcgtc accaccagcg cgaacaggcc cagcagcagc gcgaccaggg cgagcagcgg
     3481 cgtgatctgc gccgcctgga agcgggcggc gatgcggccg gagatgccgg gtcgggtgga
     3541 catgggcggt gtcgtcgatg tggttcagcg gccggcggct gcggcggctt gcggatcgag
     3601 cgcgatcgac tcgccggcgg aaacgccgct gagtacctcg acacggtcgc cccgtgccgg
     3661 accgagccgc acctggcgca gcaccggcac gccctggccg ttcaccacat agactgcggc
     3721 gagctctgcg cgccgcacga ccgcgcgcgc gggcaccagc agcgacgtgc gctcggagga
     3781 cgcctcggcg gcgatccaga cgcgcgcgaa ggcgccgggc cggacgccct ggaggccggt
     3841 gggcaggtcc agccgcactt cctgcgtgtg cgtggccgga tcgacctccg gcagccactg
     3901 cgtccgggtg ggcgtgacgc ggcgggcgcc cggcgccacg ccgagctcca cctgcgcccg
     3961 gccggtttc
//
LOCUS       DASVLN010000492         4470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_79829, whole genome shotgun sequence.
ACCESSION   DASVLN010000492 DASVLN010000000
VERSION     DASVLN010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79829"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..941
                     /locus_tag="VF453_12125"
     CDS             129..941
                     /locus_tag="VF453_12125"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX7688450.1"
                     /translation="MFERLRRARASRGSARHLADAALDDGVDALAPRLPLARLRDELV
                     ACPECDALHDRAAGDAEADRAASCGEVAPIGRHYECRRCGAPLGLERHGGFELPLSLA
                     MAGLVVFAIAQLNPVMAIDIQGQVRATTLWEAAWTLYDDGAWLLCGTVLFTTLVNPLV
                     ELAAVCYVLLPLRSGEPAPGADLVLRAMRAVRPWVMIEVFMLGALVAFTKLSAMARVT
                     PGIGLWSFGALMLLVAASASTFDHEHAWAAITAARREARAQREALRRHLADL"
     gene            complement(996..1253)
                     /gene="minE"
                     /locus_tag="VF453_12130"
     CDS             complement(996..1253)
                     /gene="minE"
                     /locus_tag="VF453_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEX7688451.1"
                     /translation="MSLFSFLLGEKKKTAAVAKERLQIILAHERTGRSKADYLPALQR
                     ELVAVISKYVSIDPNDIKVNLERQDDLEVLEVKIELPDAGR"
     gene            complement(1257..2072)
                     /gene="minD"
                     /locus_tag="VF453_12135"
     CDS             complement(1257..2072)
                     /gene="minD"
                     /locus_tag="VF453_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEX7688452.1"
                     /translation="MAKIVVVTSGKGGVGKTTTSASFSSGLALRGFKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVINGEANLHQTLIKDKQCENLFVLPASQTRDKEALTKDGV
                     ERVLKELAEMGFEYIVCDSPAGIETGALLAMHFADEALVVTNPEVSSVRDSDRIIGML
                     GSKTKRAAEGAEPVKEHLVITRYNPSRVEDGQMLSLTDIQEILRIPLLGVIPESETVL
                     QASNQGTPAIHLKDSDVSGAYLDLVARFLGEDKPMRFVDAAKPGFFKRLFGGK"
     gene            complement(2117..2881)
                     /gene="minC"
                     /locus_tag="VF453_12140"
     CDS             complement(2117..2881)
                     /gene="minC"
                     /locus_tag="VF453_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEX7688453.1"
                     /translation="MAATPTPPPTAFELTDTRLSLAVVALKSADAAALAEELAARLAE
                     TPNFFDHEPVVVDLAALREDEALPDFAEVLRILREHRMMPVMATGGSQAQMAAALDAG
                     LPAAPEPLPQPRARTVEVEVVREVEVVREVPVEVRVEVPVGVPTLVVERALRSGQQVY
                     AKGGDVVVLGAVNPGAEVLADGNVHVYGPLRGRAIAGHKGNTQARILATCLEAELVSI
                     AGVYRTSDTPLPKEVAGKPAQVRLDGDKLVMDVLKT"
     gene            2996..3367
                     /locus_tag="VF453_12145"
     CDS             2996..3367
                     /locus_tag="VF453_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688454.1"
                     /translation="MRSAPPSPTAPPPKPERWRVDAGLADVAVLAIPPDLWRTRRFEI
                     DCRLVVAPRAGGAQHAMRVEVDGALEWTRRADTANPGQTDSMDYHFRRELPAGTPLRI
                     VVKTLVRGGQRLGLTIEAEEN"
     gene            complement(3369..4361)
                     /locus_tag="VF453_12150"
     CDS             complement(3369..4361)
                     /locus_tag="VF453_12150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7688455.1"
                     /translation="MPALSVLDLVPVRAGSTPGEALRDSLALARHCEALGYGRYWVAE
                     HHNMTGIASAATSVVIGFLAQGTSTIRVGAGGIMLPNHAPLVIAEQFGTLASLYPGRI
                     DLGLGRAPGTDQTTVRALRRDPRAADTFPEDVQELQALFAPARPGQAVQAVPGAGLDV
                     PIWILGSSLYGAQLAAALGLPYVFASHFAPDALDEAMTLYRRLFKPSAQLAKPHAMAG
                     VNVVAADTDAQARRLFTTHQQSFANLRRGRPGPQQPPIDDIEAYWSPQEREMAERMLA
                     CSIVGSRETVRHGLERFAEYSGADELMVVSAIFDPEARRASYSILAEVAGLAPR"
BASE COUNT          584 a         1584 c         1677 g          625 t
ORIGIN      
        1 cgcccacgcc gcgagcgtgc gcgcgctggc ggccgacatc gccgccgcgc tgcgcgattc
       61 cgaggccgat cgcgcgcgca cctgcgcgcc ggccgcgccg cgctgaaggc cgccgccggc
      121 gacccgctat gttcgaacgc ctgcgccggg cccgcgcgtc tcgaggctcc gcccgccacc
      181 tcgccgacgc ggcgctggac gacggcgtcg acgcgctcgc gccgcgcctg ccgctcgcgc
      241 gcctgcgcga cgagctcgtc gcctgccccg agtgcgacgc gctgcacgac cgtgccgccg
      301 gcgacgccga ggccgaccgc gccgcctcct gcggcgaggt cgcgcccatc ggccgccact
      361 atgaatgccg ccgctgcggc gcgccgctgg ggctcgagcg ccacggcggc ttcgagctgc
      421 cgctgtcgct cgcgatggcc ggactggtcg tgttcgccat cgcgcaactc aacccggtca
      481 tggcgatcga catccagggc caggtgcgcg ccacgacgct ctgggaggcc gcctggacgc
      541 tctacgacga cggcgcgtgg ctgctgtgcg gcacggtgct gttcacgacg ctggtcaacc
      601 cgctggtcga gctcgcggcc gtctgctacg tgctgctgcc gctgcgctcg ggcgagcccg
      661 cgccgggcgc cgacctcgtg ctgcgcgcga tgcgcgcggt gcggccgtgg gtgatgatcg
      721 aggtcttcat gctgggcgcg ctggtggcgt tcaccaagct gtcggcgatg gcgcgcgtca
      781 cgcccgggat cgggctgtgg tccttcggcg cgctgatgct gctggtcgcc gcctcggcct
      841 cgaccttcga ccacgagcac gcgtgggccg cgatcaccgc cgcgcgacgc gaggcccggg
      901 cgcagcggga ggcgctgcgg cgccacctcg ccgacctgtg aacgcaggcg acgaacccgc
      961 gcgcgccgtc gacgcgccgg gttcgtcccg gcgcctcagc gccccgcgtc gggcagctcg
     1021 atcttcacct cgagcacctc gagatcgtcc tggcgctcga ggttgacctt gatgtcgttc
     1081 ggatcgatgg agacgtactt ggagatgacc gccaccagct cgcgctgcag cgcgggcagg
     1141 tagtcggcct tggagcggcc ggtacgctcg tgggcgagga tgatctgcag ccgctccttg
     1201 gcgacggccg cggtcttctt cttctcgccg agcaggaagg agaacaggga catcgttcac
     1261 ttccccccga acaggcgctt gaagaagccg ggcttggcgg cgtcgacgaa gcgcatcggc
     1321 ttgtcctcgc cgaggaagcg cgcgacgagg tcgaggtagg cgccggagac gtccgagtcc
     1381 ttcaggtgga tcgccggcgt gccctggttg gaggcctgca gcacggtctc cgactcgggg
     1441 atcacgccca gcagcgggat gcgcaagatc tcctggatgt ccgtcagcga cagcatctgg
     1501 ccgtcctcga cgcggctcgg gttgtagcgc gtgatgacca ggtgctcctt gacgggctcg
     1561 gcgccctcgg ccgcgcgctt ggtcttgctc ccgagcatgc cgatgatgcg gtcggagtcg
     1621 cgcaccgagg agacctcggg gttggtcacg accagcgcct cgtcggcgaa gtgcatcgcc
     1681 agcagcgcgc cggtctcgat gcccgcgggc gagtcgcaga cgatgtactc gaagcccatc
     1741 tccgcgagct ccttgagcac gcgctcgacg ccgtccttgg tgagcgcctc cttgtcgcgc
     1801 gtctgcgagg ccggcagcac gaacaggttc tcgcactgct tgtccttgat cagcgtctgg
     1861 tggaggttgg cctcgccgtt gatgacgttg atgaggtcgt agaccacgcg ccgctcgcag
     1921 cccatgatca ggtcgaggtt gcgcaggccg acgtcgaagt cgatgaccgc ggtcttgaag
     1981 ccgcgcagcg cgagccccga cgagaagctg gcgctggtgg tggtcttgcc cacgccgccc
     2041 ttgccggagg tgacgacgac gatttttgcc atgggtcgga tccttgagtc tttcggaaga
     2101 tgggtgggtg atcggatcag gtcttcagca cgtccatgac gagcttgtcg ccgtccaggc
     2161 gcacctgcgc gggcttgccg gcgacctcct tgggcagcgg cgtgtcgctc gtgcggtaga
     2221 cgccggcgat ggacacgagc tcggcctcga ggcaggtcgc gaggatgcgc gcctgcgtgt
     2281 tgcccttgtg gccggcgatc gcgcggccgc gcagcgggcc gtacacgtgc acgttgccgt
     2341 cggccagcac ctcggcgccc gggttgacgg cgccgagcac gacgacgtcg ccgcccttgg
     2401 cgtagacctg ctggcccgag cgcagcgcac gctcgacgac cagcgtcggc acccctaccg
     2461 gcacctcgac gcgcacctcg acgggcacct cgcggacgac ctcgacctcg cgcaccacct
     2521 cgacctccac ggtccgcgcg cgcggctgcg gcagcggctc gggcgcggcg ggcaggcccg
     2581 cgtcgagcgc cgcggccatc tgggcctggc tgccgcccgt ggccatcacc ggcatcatgc
     2641 ggtgctcgcg caggatgcgc agcacctcgg cgaaatcggg cagggcctcg tcctcgcgca
     2701 gggcggccag gtcgacgacg acgggctcgt ggtcgaagaa gttgggcgtc tcggccagcc
     2761 gggcggccag ctcctcggcg agggcggcgg cgtcggcgga cttcagcgcc acgacggcga
     2821 gcgacagccg ggtgtcggtg agttcgaagg cggtcggggg tggcgtcggc gtggcggcca
     2881 tcggttccgg cgaaaaaggt cgcgcacggg aaggcgcaaa gccccgaagt ttaccggccg
     2941 cgcgcgaacg ccccacggcc gggcaggccc ggggccctgc ggtatggtcg cggccatgcg
     3001 atccgcgcct ccctcgccca ccgccccgcc gcccaagccc gagcgctggc gcgtcgacgc
     3061 cggcctcgcc gacgtcgccg tgctcgccat cccgcccgac ttgtggcgca cccgccgctt
     3121 cgagatcgac tgccgcctcg tcgtcgcgcc gcgggccggc ggcgcgcagc acgcgatgcg
     3181 cgtcgaggtc gacggcgcgc tcgaatggac gcgccgcgcc gacacggcca atcccgggca
     3241 gaccgactcg atggactacc acttccgccg cgagctgccc gcgggcacgc cgctgcgcat
     3301 cgtcgtgaag acgctggtgc gcggcgggca gcgactcggc ctgacgatcg aggccgagga
     3361 gaactgactc agcgcggcgc gaggccggcg acttcggcaa ggatcgagta ggacgcgcgc
     3421 cgagcctcgg gatcgaagat cgccgagacg accatcagct cgtcggcgcc cgagtactcg
     3481 gcgaagcgct cgagcccgtg gcgcacggtc tcgcgcgaac cgacgatcga gcaggcgagc
     3541 atgcgctcgg ccatctcgcg ttcctgcggg ctccagtagg cctcgatgtc gtcgatgggc
     3601 ggctgctgcg ggccggggcg gccgcggcgc aggttggcga aggactgctg gtgcgtcgtg
     3661 aacaggcgcc gcgcctgcgc gtcggtgtcc gcggcgacga cgttgacgcc ggccatcgcg
     3721 tgcggcttcg cgagctgcgc cgagggcttg aacagccggc ggtagagcgt catcgcctcg
     3781 tcgagcgcgt cgggcgcgaa gtgcgacgcg aagacgtagg gcaggcccag cgccgcggcg
     3841 agctgcgcgc cgtagaggct ggagccgagg atccagatcg gcacgtccag gcccgcgccg
     3901 ggcaccgctt gcacggcctg gccgggacgc gcgggcgcga acagggcctg cagctcctgc
     3961 acgtcttccg ggaacgtgtc ggcggcgcgc gggtcgcggc gcagggcgcg cacggtcgtc
     4021 tggtcggtgc cgggcgcgcg gcccaggccc aggtcgatgc ggccggggta gagcgaggcg
     4081 agcgtgccga actgctcggc gatcacgagc ggcgcgtggt tgggcagcat gatgccgccg
     4141 gcgccgacgc ggatcgtcga ggtgccctgg gcgaggaagc cgatcaccac cgacgtcgcc
     4201 gcgctcgcga tgcccgtcat gttgtgatgc tcggcgaccc agtagcggcc atagccgagc
     4261 gcctcgcagt ggcgcgcgag cgcgaggctg tcgcgcagcg cctcgccggg ggtcgagccg
     4321 gcgcgcacgg gcacgaggtc gaggacggaa agcgcgggca gcttgggcag agagatcgag
     4381 gacatggagg aggtggcggg aacgaagccg cacgatacgc cgcgcggccc gcggctgccg
     4441 cggcccgccg cgagaccgcg ggccgcgtgg
//
LOCUS       DASVLN010000493         4415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_456220, whole genome shotgun sequence.
ACCESSION   DASVLN010000493 DASVLN010000000
VERSION     DASVLN010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456220"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2330)
                     /locus_tag="VF453_12155"
     CDS             complement(<1..2330)
                     /locus_tag="VF453_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEX7688456.1"
                     /translation="MTRQDPHLSFDSGAHGRSPAQVEEDRLQALADGLAARDVDAEAA
                     AAQAQAQALLQSVGVDVDALLQAMVYVTRHHGKERSADSLLDGMPVDGALGPDQAVRV
                     MRAAGYHAGILKRPIGDIHALLLPAILLLKNGDACVLVRRFDDTGLCEVVMPGPDFHV
                     VRASEEELSAEYLGFTLVATPFPEQAPGLDPQDRALLDPNGHWLWGTIRRFVPYYRSA
                     MLAALLSNVLMLVSGLVTSVVYDKVIPHQAFVTLWALAGGAAMALGFDLLSRQLRSHL
                     IDTAGKKADLIIGALLFRQTLAVRMEHRPESAGGYAHHMAQVEIVRDFFAGATLSAVS
                     DLPFIFMYIAMTFVIGGPLGWVLVLAVPALMGMAFGVQKGLRRAMSANMAHQADLQGV
                     VVEAVEGLEDLKASGAQGRFLHRYESATQAAAETALKARWMSSWTSNMSMVSQQLVTL
                     VMLVWGVYLIHDSVVTGGALIGAVMYATRAVAPINSVVGLATRYQGARAALVSLDKLM
                     AQPTEREAGRTYITGHHVSGRIGLHEAGFSYPPANGAPAPQVLKGVTLGIQAGERIAI
                     LGRIGSGKSTVLRLMAGLYQPTSGMVEVDGLDLRQIDPADYRGRVGFVSQEPRLFNGS
                     LRDNVVMGRPGVDAARLTEVARITGLDRVVAGHPQGWELPVGEMGGLLSGGQRQLVAL
                     ARCLVTRPQILLMDEPTSSMDAQSEIMFLRQLKEAAGTRTLVMVTHRPAVLELVDRIL
                     VVDGGKLVMDGPKAAVLAALSGVRPPAPPQGTPSVA"
     gene            complement(2343..4190)
                     /locus_tag="VF453_12160"
     CDS             complement(2343..4190)
                     /locus_tag="VF453_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglucosamine transferase"
                     /protein_id="HEX7688457.1"
                     /translation="MNATPAMIPPSADGELERDLARARAGDMPLAQLLDRATGLSQRG
                     LAEASATLYEQWIAGTDSPMRHVACFNWGTVLAAMGRHAQAEQAYVRALEFKPDFLQA
                     ALNRGHQLEQLKRHDEALAQWTRVAELADAANDPDLQLRLHALNNAARMLETQRRFAE
                     SERLMVRSLELKADQPDVLQHYVHLRQKQCAWPIHQPVGAVTRNQLLTCTSPLAMLSA
                     TDDPALHLLAAQRFAYERIAKPPTTHLYRTGPKREGRIRVGYLSGDLCLHAVGLLVPE
                     LLELHDRERFEVFAFCWSREDGSAQRRRLVAGVDHLVRIDRFDDVTAARLIAGHGIDI
                     LVDLQGLTSGARPGILGMRPAPVQVSYLGFPGCSGIPGVEWIIADRFVMPPEQLPYST
                     EKPLYLDRCYQVSDRRREIGAIPDRARYGLPEDKFVFCSFNNNFKFTEDVFDAWMRIL
                     RATGDSVMWLLADNDPARENMLARAAAHGVDPARLRFAPRVSPADYLARFTLADLVLD
                     TFPYNAGTTASDALWMGTPLLTLSGRSYISRMAGSLLTHVGLPELVTHTLADYERLAI
                     QLAGQPARIASYKRYLAEHGRASALFDVPAFVRELEAEFARLALAARAA"
BASE COUNT          616 a         1519 c         1682 g          598 t
ORIGIN      
        1 tgcgcgacgg acggcgtgcc ctgcggcggc gccggcgggc gcacgccgga cagcgcggcc
       61 agcacggcgg ccttcgggcc gtccatcacg agcttgccgc cgtcgacgac gaggatgcgg
      121 tcgaccagct ccagcaccgc gggccggtgc gtgaccatga ccagcgtgcg ggtgcccgcg
      181 gcctccttga gctggcgcag gaacatgatc tcggactggg cgtccatcga gctggtgggc
      241 tcgtccatca gcaggatctg cggacgcgtg accaggcagc gcgcgagcgc cacgagctgg
      301 cgctggccgc cggagagcag gccgcccatc tcgccgaccg gcagctccca gccctgcggg
      361 tggccggcga cgacgcggtc gaggcccgtg atgcgcgcga cctcggtcag gcgcgcggcg
      421 tcgaccccgg gccggcccat cacgacgttg tcgcgcagcg agccgttgaa caggcgcggc
      481 tcctgcgaca cgaagccgac gcggccgcgg tagtcggccg gatcgatctg gcgcaggtcg
      541 aggccgtcga cctccaccat gcccgaggtc ggctggtaga ggcccgccat caggcgcagc
      601 accgtcgact tcccgctgcc gatgcgcccg aggatggcga tgcgctcgcc cgcctggatg
      661 cccagcgtga cgcccttgag cacctgcggc gccggcgcgc cgttggccgg cgggtaggag
      721 aagccggcct cgtgcaggcc gatgcggccg ctgacgtggt gcccggtgat gtaggtgcgg
      781 ccggcctcgc gctcggtggg ctgggccatc agcttgtcca gcgagacgag cgcggcgcgc
      841 gcgccctggt agcgcgtcgc caggccgacg acgctgttga tcggcgcgac ggcgcgcgtc
      901 gcgtacatca cggcgccgat cagcgcgccg ccggtgacga cgctgtcgtg gatcaggtac
      961 acgccccaca ccagcatcac cagcgtgacc agctgctgcg agaccatgga catgttgctg
     1021 gtccagctcg acatccagcg cgccttcagg gcggtctcgg cggccgcttg cgtggcgctc
     1081 tcgtagcggt ggaggaagcg gccctgcgcg cccgaggcct tcagatcctc caggccctcg
     1141 acggcctcga ccaccacgcc ctgcaggtcg gcctggtgcg ccatgttggc gctcatcgcg
     1201 cgtcgcaggc ccttctgcac gccgaaggcc atgcccatca gcgccggcac ggcgagcacc
     1261 agcacccagc cgagcggccc gccgatgacg aaggtcatcg cgatgtacat gaagatgaaa
     1321 ggcaggtcgc tcacggccga cagcgtcgcg ccggcgaaga agtcgcgcac gatctcgacc
     1381 tgcgccatgt ggtgcgcgta gccgccggcg gactcgggcc ggtgctccat gcgcacggcc
     1441 agcgtctggc ggaacagcag cgcgccgatg atcaggtcgg ccttcttgcc cgcggtgtcg
     1501 atcaggtggc tgcgcagctg gcgcgagagc aggtcgaagc cgagcgccat cgccgcgccg
     1561 ccggccagcg cccacagcgt gacgaaggcc tggtgcggga tgaccttgtc gtagacgacc
     1621 gaggtcacga gcccgctcac gagcatcagc acgttgctca ggagcgcggc cagcatcgcc
     1681 gagcggtagt acggcacgaa gcggcggatc gtgccccaca gccagtgtcc gttcggatcg
     1741 agcagcgcgc gatcctgggg gtcgagtccg ggcgcctgct cggggaacgg cgtggccacg
     1801 agcgtgaagc cgaggtactc ggccgacagc tcctcctccg aggcgcgcac gacgtggaag
     1861 tccgggcccg gcatcacgac ctcgcacagg ccggtgtcgt cgaaacggcg cacgagcacg
     1921 caggcgtcgc cgttcttcag cagcaggatg gcgggcagca gcagcgcgtg gatgtcgccg
     1981 atcgggcgct tgaggatgcc cgcgtggtag cccgccgcgc gcatcacgcg cacggcctgg
     2041 tccggaccga gcgcgccgtc gacgggcatg ccgtcgagga gcgaatcggc cgagcgctcc
     2101 ttgccgtggt ggcgcgtcac gtagaccatc gcctgcagca gcgcgtcgac gtcgacgccc
     2161 accgattgca gcagcgcctg cgcctgggcc tgcgccgccg cggcctcggc gtcgacgtcg
     2221 cgcgcggcca ggccgtcggc cagcgcctgc aggcgatcct cctcgacctg cgcgggcgag
     2281 cggccgtgcg cgccggagtc gaagctgagg tgaggatcct ggcgggtcat gggggcgtcc
     2341 ggtcaggcgg cacgggcggc gagcgcgagg cgggcgaact ccgcctcgag ctcgcgcacg
     2401 aaggcgggca cgtcgaacag cgccgaggcg cggccgtgct cggcaaggta gcgcttgtac
     2461 gacgcgatgc gcgccggctg gccggcgagc tggatcgcga ggcgctcgta gtcggccagc
     2521 gtgtgcgtca cgagctcggg caggcccacg tgcgtgagca ggctgccggc catgcgcgag
     2581 atgtagctgc ggcccgacag cgtgagcagc ggcgtgccca tccacagcgc gtcgctggcg
     2641 gtggtgccgg cgttgtacgg gaaggtgtcg agcaccaggt cggccagcgt gaagcgggcg
     2701 aggtagtcgg ccgggctgac gcgcggcgcg aagcgcaggc gtgcgggatc gacgccgtgc
     2761 gcggcggcgc gggcaagcat gttctcgcgc gcgggatcgt tgtccgcgag cagccacatc
     2821 acgctgtcgc cggtggcgcg caggatgcgc atccacgcgt cgaacacgtc ctcggtgaac
     2881 ttgaagttgt tgttgaacga gcagaagacg aacttgtcct cgggcaggcc gtagcgcgcg
     2941 cgatccggga tcgcgccgat ctcgcgccgg cggtcgctca cctggtagca gcggtcgagg
     3001 tagagcggct tctcggtgct gtagggcagc tgctcgggcg gcatcacgaa acggtcggcg
     3061 atgatccact cgacgcccgg gatcccggag cagcccggga agccgaggta gctcacctgc
     3121 accggcgcgg gccgcatgcc gaggatgccg ggccgcgcgc cgctggtcag tccctgcagg
     3181 tcgaccagga tgtcgatgcc gtggccggcg atcaggcgcg ccgcggtgac gtcgtcgaag
     3241 cggtcgatgc gcacgaggtg gtcgacgccg gcgacgaggc gcctgcgctg cgcgctgccg
     3301 tcctcgcggc tccagcagaa cgcgaacacc tcgaagcgct cgcggtcgtg cagctcgagc
     3361 agctcgggca ccagcaggcc gacggcgtgc aggcacaggt cgcccgagag gtagcccacg
     3421 cggatgcgcc cttcccgctt cggaccggtg cgatacaggt gcgtggtggg cggcttggcg
     3481 atgcgctcgt aggcgaagcg ctgcgccgcc agcaggtgga gcgcgggatc gtcggtggcg
     3541 ctgagcatgg ccagcggcga ggtgcaggtc agcagctggt tgcgcgtgac cgcccccacc
     3601 ggctggtgga tcggccacgc gcactgcttc tggcgcaggt gcacgtagtg ctgcagcacg
     3661 tcgggctggt cggccttcag ctcgaggctg cgcaccatca ggcgctcgga ctcggcgaag
     3721 cggcgctgcg tctccagcat gcgcgcggcg ttgttcagcg catgcaggcg cagctgcagg
     3781 tcgggatcgt tggcggcatc ggcgagctcc gcgacacgcg tccactgcgc gagcgcctcg
     3841 tcgtggcgct tgagctgctc gagctggtgg ccgcggttga gcgcggcctg caggaagtcg
     3901 ggcttgaatt cgagcgcgcg cacgtaggcc tgctcggcct gcgcgtggcg gcccatggcg
     3961 gccaggacgg tgccccagtt gaagcacgcg acgtggcgca tcggcgagtc ggtgccggcg
     4021 atccactgtt cgtacagcgt cgccgaggcc tcggcgagcc cgcgctggct caggccggtg
     4081 gcgcggtcga gcagctgcgc gagcggcatg tcccccgcgc gcgcccgggc aaggtcgcgc
     4141 tcgagctcgc cgtcggccga cggcgggatc atggcggggg tggcgttcat cgcgacagcg
     4201 gtggagattc ggatgccacc gattctggag cccgcccggc tcggccttta gctcgatcag
     4261 cggggccaat gcggccctat tcgcatcacg gggccgcgcg ccgcggccac cccggcgaag
     4321 gcgcggcggc gggccgggtc gccaccccgc ccggccgtgg aaaaggggga cgggcgcgtg
     4381 ccccgtcccg cttcagcgct tgctgcgctt ggctg
//
LOCUS       DASVLN010000494         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_79965, whole genome shotgun sequence.
ACCESSION   DASVLN010000494 DASVLN010000000
VERSION     DASVLN010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79965"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VF453_12165"
     CDS             complement(<1..889)
                     /locus_tag="VF453_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7688458.1"
                     /translation="MLKPATAWSLARETLAAWSEDYAASMGAALAYYTMFSIAPLLLI
                     VISVAGLAFGEQAARGEILGQLESLMGSEGAHAVQALLASLDKPTTGLWATAFGVGAL
                     VVGATTVIGELQDALDRIWRVPGRGTGGGAWAWLRRRLLSLGMILGIGFLMMVSLVAS
                     AALAAIQRWWGRYADLGALGQAVDFMLSFAFITVAFAMIYKLMPRARVQWRDVWIGAV
                     ATALLFSVGKTAIGLYIGRSAVASAFGAAASLVAMVVWVYWSAQIFLLGAEFTWVYAH
                     RLGSRAGRAPLAPGAVPARA"
     gene            complement(978..1433)
                     /locus_tag="VF453_12170"
     CDS             complement(978..1433)
                     /locus_tag="VF453_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEX7688459.1"
                     /translation="MDNQDTIDVLNTLIETCNDGERGFRNVAEHVHSADLKTLFTNRA
                     EDCRQACADLRAQVLALGGDPETGGSASGALHRGWVSVLGTLTGYSDKSLLEACEKGE
                     DSAMERYRDALDQPLPDNIRLLIERQYEGVKRNHAQVRALRDQARAMSN"
     gene            complement(1510..>2732)
                     /locus_tag="VF453_12175"
     CDS             complement(1510..>2732)
                     /locus_tag="VF453_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX7688460.1"
                     /translation="AFPPGTAADPAHAHALVAGLDEPLVAGNRVDLLQDGPGTYAAMF
                     AAIDGARDHVNLESYILEAEGPGEEFARRLVDKARAGVRVNVLYDSLGSLATPGRYFE
                     ELRAGGVSVCEYNPLRRLGTLFSRALHLRDHRKLMVVDGRIGFIGGVNISTVYSAGSS
                     PLASVAGSDGPSDDAVEPGWRDTHVRVEGPIVAQLQRLFIAHWNRFSTCALRGANYFP
                     PIAPAGTRRVAVAACDAGRRRNPFYRALLAAINAANHRIWLTTAYLVPTRRLARALVR
                     AARRGVDVQLLLPGKSDFWAPLQAGRSHYGRLLRAGIRLHELHDTLLHAKTCVIDGLW
                     TTVGSSNLDWRSFLHNAEANLVVLDAGLGREMEETFARDLERAREIDRAAWARRGLRQ
                     RLAEAIARRFEFFL"
BASE COUNT          399 a          986 c          994 g          353 t
ORIGIN      
        1 gcgcacgcgc cggcacggcg cccggcgcca gcggcgcgcg tccggcccgc gagcccaggc
       61 ggtgggcata gacccaggtg aactcggcgc cgaggaggaa gatctgcgcc gaccagtaga
      121 cccagacgac catcgccacc agcgaggccg cggctccgaa ggccgaggcc accgcgctgc
      181 ggccgatgta cagcccgatg gcggtcttgc cgacgctgaa cagcagcgcc gtcgcgaccg
      241 caccgatcca cacgtcgcgc cactgcaccc gcgcgcgcgg catgagcttg tagatcatcg
      301 cgaacgcgac cgtgatgaag gcgaagctga gcatgaagtc gaccgcctgc ccgagcgcgc
      361 cgaggtcggc gtagcggccc caccagcgtt gaatggccgc gagcgccgcg ctcgccacca
      421 gcgaaaccat catcaggaag ccgatgccca ggatcatccc gagcgagagc aggcgccggc
      481 gcagccaggc ccaggcgccg ccgccggtac cgcggccggg gacgcgccag atgcggtcga
      541 gcgcgtcctg cagctcgccg atcacggtcg tggcgccgac caccagcgcg ccgaccccga
      601 acgccgtggc ccagagcccg gtcgtgggtt tgtccaggct cgcgagcagc gcctgcaccg
      661 cgtgcgcgcc ctcgctgccc atcagcgatt cgagctggcc gaggatctcg ccgcgcgccg
      721 cctgctcccc gaacgcgagc ccggcgaccg agatcacgat cagcagcagc ggcgcgatcg
      781 agaacatcgt gtagtaggcg agcgccgcgc ccatgctggc ggcgtagtcc tcggaccagg
      841 ccgccagcgt ctcccgcgcg agcgaccagg cggtggcggg cttcaacatc gcgcgatcct
      901 tccgatacgg gcccccgtat gacgcgcggc cgccccggcg aaggggcgac cgcgcgaagg
      961 cgagggcgct cgccgcgtca gttcgacatc gcgcgcgcct ggtcgcgcag cgcgcgcacc
     1021 tgcgcgtggt tgcgcttgac gccttcgtac tggcgctcga tcagcaggcg gatgttgtcc
     1081 ggcagcggct ggtcgagggc gtcgcgatag cgctccatcg cggagtcctc gcccttctcg
     1141 caggcctcga gcagcgactt gtcgctgtag ccggtcagcg tgcccagcac cgagacccag
     1201 ccgcgatgca gcgcgccgct ggcgctgccg ccggtctccg gatcgccgcc cagggccagc
     1261 acctgggcgc gcaggtcggc gcaggcctgg cggcagtcct cggcgcggtt cgtgaacagc
     1321 gtcttcaggt cggccgaatg cacgtgctcg gcgacgttgc ggaagcctcg ctcgccgtcg
     1381 ttgcaggtct cgatgagcgt gttgaggacg tcgatggtgt cttggttgtc catgggatca
     1441 cctctgttga ttgggggtgg agataccccc tacgggcaga tgccgtgccc atgcggcgcc
     1501 tgcgcaagcc tacaggaaga actcgaagcg ccgcgcgatc gcctcggcga gccgctggcg
     1561 cagcccgcgc cgcgcccatg cggcccggtc gatctcgcgc gcgcgttcga ggtcgcgggc
     1621 gaaggtctcc tccatctcgc ggccgagtcc ggcgtcgagc acgaccaggt tggcttcggc
     1681 gttgtgcagg aagctgcgcc agtccaggtt gctcgaaccc accgtcgtcc acaagccgtc
     1741 gatgacgcag gtcttcgcgt gcagcaaggt gtcgtgcagt tcgtgcagcc ggatcccggc
     1801 gcgcaggaga cggccgtagt gcgagcgccc ggcctgcagc ggcgcccaga aatcgctctt
     1861 gcccggcagc agcagctgca cgtcgacgcc gcggcgcgcg gcgcgcacga gcgcgcgcgc
     1921 gagccgccga gtcggcacga gataggcggt cgtcagccag atgcggtggt tggcggcgtt
     1981 gatggccgcg agcagcgcgc ggtagaacgg attgcgccgc cgccccgcgt cgcaggccgc
     2041 gaccgcgacc cgccgcgtgc ccgccggggc gatcggcggg aagtagttcg cgccgcgcag
     2101 cgcgcaggtg gagaaccggt tccagtgcgc gatgaacagg cgctgcagct gcgcgacgat
     2161 ggggccctcg acgcgcacgt gggtgtcgcg ccagccgggc tccaccgcgt cgtcggaggg
     2221 gccgtccgag ccggcgacgc tcgccagcgg cgacgagccg gccgagtaca ccgtgctgat
     2281 gttgacgccg ccgatgaagc cgatgcggcc gtcgaccacc atcagcttgc gatggtcgcg
     2341 caggtgcagc gcgcggctga acagcgtgcc caggcggcgc agcgggttgt attcgcacac
     2401 gctcacgccg ccggcgcgca gctcctcgaa gtaccggccg ggcgtggcga gcgagcccag
     2461 gctgtcgtag agaacgttca cgcgcacccc ggcgcgcgcc ttgtcgacca gccgacgcgc
     2521 gaactcctcg ccggggccct cggcctcgag gatgtagctc tccagattga cgtggtcgcg
     2581 cgcaccgtcg atggccgcga acatcgccgc gtaggtgccg gggccgtcct gcagcaggtc
     2641 cacgcggttg ccggcgacga gcggctcgtc gagcccggcg accagcgcat gcgcgtgcgc
     2701 cggatcggcc gccgtgccgg gcgggaacgc gg
//
LOCUS       DASVLN010000495         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_239956, whole genome shotgun sequence.
ACCESSION   DASVLN010000495 DASVLN010000000
VERSION     DASVLN010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239956"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VF453_12180"
     CDS             complement(<1..301)
                     /locus_tag="VF453_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7688461.1"
                     /translation="MPARRQTLPAESARDVDDDARAVRVLRQFRQVFNAVKTHFQQVE
                     KSVGMGGAQVWALSLVRDRPGLGVRALAEAMAVHQSTASNLVRGLVERELVATTKD"
     gene            complement(465..1043)
                     /gene="pal"
                     /locus_tag="VF453_12185"
     CDS             complement(465..1043)
                     /gene="pal"
                     /locus_tag="VF453_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX7688462.1"
                     /translation="MSQAELDRPSFAARAVRSAGMIVLPVAVAALAACASKPVAPPAP
                     TTASSSTTTTTHTPAPATTPAPQATALAAYLDPNSPISQRRSVFFDFDESTLIGDDKA
                     VIELQGDYLAKHPDVHVRVEGNTDERGSDEYNLALGQRRAQAVVSAMQLVGAKSGQME
                     AVSYGKEKPKATGHDEAAWAQNRRADIVYPSH"
     gene            complement(1313..2716)
                     /locus_tag="VF453_12190"
     CDS             complement(1313..2716)
                     /locus_tag="VF453_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX7688463.1"
                     /translation="MHHRHTATPKFDKLTHHHEHRPDVGAVDDPDVDVGTLMARAQRH
                     AFGYFEQGTDPASGLVLDSTQPDSPCSIMAVGLALTAYPIAVERGWMTRLQAIAVTLR
                     TLRFFHEADMDGRPDSTGYRGFYFHFLDMHTGRRTWNSELSSIDTTFLVAGFKIARLY
                     FDRDSGHEREIRERADALVARVDWAWMQNGDDALCHGWKPERGFLRYRWSGYNEGLLM
                     LMLALGASERPASAACWEKFVGGYKWRTIYGHSHLTAGPLFIHQYSHMWVDFRGIQDD
                     FMRAHGIDYFENSRRATLVHREYAIRNPKHFKGYCANCWGLSACDGPGPRDVVVGGRK
                     RKLLGYAARGAPYGPDDGTVAPYASVASLPFAPDVVVPTMQHIARMVHAHKTYRPGNS
                     FNPTWHDADGPPGWISPWRFALNSGPVVLMIENFLTGLPWRLMRRDEELVRGLRRAGF
                     RGGWLEGEDGPSGPGSD"
     gene            complement(3096..3695)
                     /locus_tag="VF453_12195"
     CDS             complement(3096..3695)
                     /locus_tag="VF453_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688464.1"
                     /translation="MDRTFLMTALVAGAALGASFGACAQATAAGSVQRDANQEQRIEN
                     GLRNGSITPREDALLQRDEAHVDKLQSRAMRDGTLSAADKARLNAAQDKAGRDIATAT
                     HNGVEGNPMSASSQRAQAEAQRDVNQQKRIAAGVRDGSLTKHESARLENGQARVDKAE
                     YRAGRDGRVGAGEQTRVARAQNHQSVRIRRQRHDAQHRG"
     gene            3909..4133
                     /locus_tag="VF453_12200"
     CDS             3909..4133
                     /locus_tag="VF453_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEX7688465.1"
                     /translation="MPTDDDIDRRLTDLEVKASFAEDTLDALNEVILRQQRQIELLLR
                     EVATLKDRAPDAGAAGAGDRDPREEIPPHY"
BASE COUNT          555 a         1448 c         1569 g          652 t
ORIGIN      
        1 cgtccttggt cgtggccacg agctcgcgct ccaccagtcc gcgcacgagg ttgctcgcgg
       61 tcgactggtg caccgccatc gcctccgcga gcgcgcgcac gcccaggccg gggcggtcgc
      121 gcacgaggct gagcgcccag acctgggctc cgcccatgcc gaccgacttc tcgacctgct
      181 ggaagtgcgt cttgacggcg ttgaagacct gccggaactg gcgcagcacc cggacggccc
      241 gggcgtcgtc gtcgacgtcg cgggcacttt cggcggggag ggtctggcgg cgagcgggca
      301 tgcgtcgatg atgcgggcgg tagaccggcg cccgccgggc ggcgaggccc ggcgcgcggc
      361 gcgcaaaaaa aaggacgccc gggtgggcgt cccaagctag gtggtcaccc agcgctcccg
      421 aaagcggccg gcgcaccaca cgccggccca cacacactcg gtcatcaatg cgaggggtac
      481 acgatgtccg cgcggcggtt ctgcgcccac gccgcttcgt cgtgacccgt cgccttgggc
      541 ttttccttcc cgtagctcac cgcttccatc tgtccggact tggcgccgac gagctgcatc
      601 gccgagacca ccgcctgcgc gcggcgctgg cccagggcca ggttgtattc gtcgctgccg
      661 cgctcgtcgg tgttgccctc gacgcgcacg tgcacgtccg ggtgcttggc gaggtagtcg
      721 ccctgcagct cgatcaccgc cttgtcgtcg ccgatgagcg tgctctcgtc gaagtcgaag
      781 aacacgctgc gccgctgcga gatcgggctg ttcggatcca ggtaggccgc gagcgccgtc
      841 gcctgcgggg ccggcgtggt ggccggcgcg ggcgtgtggg tggtggtcgt cgtcgagctg
      901 gacgcggtcg tcggggcggg cggcgccacc ggcttgctcg cgcaggcggc cagggcggcg
      961 acagccaccg gcaggacgat catcccggcg ctgcggacgg cgcgggcggc gaaggaggga
     1021 cggtcgagtt cggcttggga catgacgagg tggccgctcg gggcggctgg ttgacggaca
     1081 ctcctcgaca ataccagata catttgtgca aatctattgc gtgccttgcg gcgggcttgc
     1141 gtgtctctcg tccgtacgaa ccgccgggtg tgtggagctt gcccggcggg tgccggccgc
     1201 gcccatgggg ggtggagtga gctgcggacg attggttccg tgaattcccg gccctgcggc
     1261 acgcccgtgg acgtggccgg ggcgcgcccg cggcgacgag cccgcggccc ggtcagtcgc
     1321 tgcccgggcc gctcggtccg tcctcgccct cgagccagcc gccccggaag cccgcgcggc
     1381 gcaggccgcg cacgagttcc tcgtcgcgac gcatcaggcg ccagggcagc ccggtgagga
     1441 agttctcgat catcagcacg accggcccgc tgttgagggc gaagcgccag ggcgagatcc
     1501 agcccggcgg gccgtcggcg tcgtgccagg tcgggttgaa cgagttgccg ggccggtagg
     1561 tcttgtgggc gtgcaccatg cgtgcgatgt gctgcatggt cggcacgacg acgtcgggcg
     1621 cgaagggcag cgaggccacc gaggcgtagg gcgcgaccgt gccgtcgtcc ggaccgtagg
     1681 gcgcgccgcg ggcggcgtag ccgaggagct ttcgcttgcg cccgccgacc acgacgtcgc
     1741 gcggccccgg cccgtcgcaa gccgacaggc cccagcagtt ggcgcagtag cccttgaagt
     1801 gcttggggtt gcggatcgcg tactcgcggt gcacgagggt cgcgcggcgg ctgttctcga
     1861 agtagtcgat gccgtgcgcg cgcatgaagt cgtcctggat gccgcggaaa tccacccaca
     1921 tgtgcgagta ctggtggatg aacagcggcc cggccgtcag gtgcgaatgc ccgtagatcg
     1981 tgcgccactt gtagccgccg acgaacttct cccagcacgc cgcgctggcc ggccgttcgc
     2041 tcgcgccgag ggcgagcatc agcatcagca gcccctcgtt gtagccgctc cagcgatagc
     2101 gcaggaagcc gcgctcgggc ttccagccat ggcacaaggc gtcgtcgccg ttctgcatcc
     2161 aggcccagtc gacgcgcgcc acgagcgcgt cggcgcgctc gcggatctcg cgttcgtggc
     2221 cgctatcgcg gtcgaagtac agccgcgcga tcttgaaccc ggcgacgagg aaggtggtgt
     2281 cgatgctcga gagctcgctg ttccacgtgc gccgcccggt gtgcatgtcc aggaagtgga
     2341 agtagaagcc gcggtagccg gtggagtcgg gacggccgtc catgtccgcc tcgtggaaga
     2401 agcgcagcgt gcgaagcgtg accgcgatcg cctgcaggcg cgtcatccag ccgcgctcca
     2461 cggcgatcgg gtaggcggtc agcgcgaggc cgacggccat gatgctgcag gggctgtccg
     2521 gctgggtgga gtcgagcacg aggccgcttg ccggatccgt gccctgctcg aagtagccga
     2581 aggcatggcg ctgcgcgcgc gccatcagcg tgcccacgtc gacgtccgga tcgtcgaccg
     2641 caccgacgtc ggggcggtgc tcgtggtgat gcgtcagctt gtcgaatttc ggggtcgcgg
     2701 tgtggcgatg gtgcatcggg cgtcggggat gacggagcgc ggcggaggcc gatcgctgcc
     2761 gggcagagcg cgcctcggca ctcggcgtgc ccacctccgc gaaggtcgcc cggcggcctt
     2821 gcgcactgcc cgcggcgccg gcccccagcg cgacagcttg ccttcagtcc gacgagggaa
     2881 aacacccgcg ccggggccgt tctcgcgtcg aacaatgaag tcggaacttt cgctcgcatc
     2941 gagctccgcg ccgcgcgcgc agtgtccgcg gcgggcgaag tcgtccggcg tcgacgacga
     3001 cgggcggccc ctcccgaggg gatgttccgg ggtgaaactt tgggggccgg cgtcctggga
     3061 cgaccggccc ttctttcgcc tcgcgccgat cgccctcagc cgcgatgctg cgcgtcgtgg
     3121 cgctggcgcc ggatgcggac gctctggtgg ttctgcgcgc gcgccacgcg ggtctgctcg
     3181 ccggcgccga cgcggccgtc gcggcccgcc cggtattcgg ccttgtcgac gcgcgcctgg
     3241 ccgttctcca gcctcgcgga ctcgtgcttc gtgaggctgc cgtcgcgcac gcccgcggcg
     3301 atgcgctttt gctggttcac gtcgcgctgt gcctcggcct gagcgcgctg ggaggaggcc
     3361 gacatcggat tgccctcgac gccattgtgc gtggccgtgg cgatgtcgcg gccggccttg
     3421 tcctgcgccg cgttcagccg tgccttgtcg gcagccgaca gcgtgccgtc gcgcatcgcc
     3481 ctggactgca gcttgtcgac gtgcgcttcg tcgcgctgga gcagggcgtc ctcgcggggg
     3541 gtgatgctgc cattgcgcaa gccgttctcg atgcgctgtt cctggttcgc gtcgcgctgc
     3601 acgctgcccg cggcggtcgc ctgtgcgcag gcgccgaagc tcgcgccgag ggcggcgccg
     3661 gcgacgaggg cggtcatgag gaaggtgcgg tccatggtgt gtgatcctgt tcctgagagt
     3721 cgttgatcgg tggacgggaa cgcgcgtttc gtacgggttc aacgggctcc gccaagaggg
     3781 agttgacccg gcgaccccga catcacctgc ggatgcacgc gcttgacgaa atggaaacac
     3841 ggctgcgcag ccaccccgcg cccgcggagg gcaggcggcg accgcgggcc atgggttcca
     3901 atacgggcat gcccaccgac gacgacatcg accgccgcct gaccgacctc gaggtcaagg
     3961 ccagcttcgc cgaggacacg ctcgacgccc tcaacgaggt catcctgcgc cagcagcgcc
     4021 agatcgagct gctgctgcgc gaagtcgcga ccctgaagga ccgcgcaccc gacgcgggcg
     4081 ccgccggcgc gggcgaccgc gacccgcgcg aggagatccc gccgcactat tgatcgcgcg
     4141 cacaccggat acgggcaggc gtatgcccgc cccgcgacgc ttcaggagcg gtcggaggcg
     4201 attgcgacgg cgccctggtg cgcg
//
LOCUS       DASVLN010000496         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_295058, whole genome shotgun sequence.
ACCESSION   DASVLN010000496 DASVLN010000000
VERSION     DASVLN010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295058"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF453_12205"
     CDS             complement(<1..189)
                     /locus_tag="VF453_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688466.1"
                     /translation="MFSFFRKKTDPAASPAGPAAEPPPAPSAPLEFLKRVFTGAPAPA
                     AGVVEPVPEAVEAPPAAAP"
     gene            373..449
                     /locus_tag="VF453_12210"
     tRNA            373..449
                     /locus_tag="VF453_12210"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:407..409,aa:Met,seq:cat)
     gene            542..1495
                     /locus_tag="VF453_12215"
     CDS             542..1495
                     /locus_tag="VF453_12215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7688467.1"
                     /translation="MLPQRIGRYDVCAELGRGSTSIVYKGVDPQSRRFVAIKTLQRDG
                     SDEEADADTILRRFRNEARTIGRLRHPGVVAVDEFGEDGLHSWIAMEYVEGRNLDDVL
                     LRSPVLGQERVLAIMDELLDALGCVHREGICHRDVKPANIMVTPEGRVKLTDFGVARL
                     RDLGLTRVSSVIGTPAFMAPEQFTGGALDHRADLFACGVLAYLLLSGRRPFSGAPAAV
                     MHQILHEEPPPLSDAGGGLVGSAYDDVIARALAKRADARFSSAEEMRAALRDAARRAA
                     ESPLATIVRTMVQDVGSRPGPGGDRPRDERADPSQPGPFGR"
     gene            complement(1471..2430)
                     /locus_tag="VF453_12220"
     CDS             complement(1471..2430)
                     /locus_tag="VF453_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7688468.1"
                     /translation="MGTLFDPRTGERVVLHAEHVFGRNALRADTALDDPGISLMHAVA
                     RWRDGRWMLSDHSRNGTFVDGQVLVPGEPFPLAVGAEVRLGRGTGVAWRVQDVDAPVD
                     ALVPPDSRQPVIVLAPHNLLPSSEAPELCVYQARPGLWMLEHNGETRALKDGDRVQVG
                     AAGLHRFVAAAAMDDTQVSEVADEPEAPWLVMRLSLDEEHACLELRDGATRTDLGERS
                     HHYCLATLARRRMADRERGLEPGAQGWLSSAELSRMLGMETTHLNMQIFRAREQLMTA
                     LPGAAALSRLVERRRGELRIGDLPFEIYRGDRLECRYRPNGPG"
     gene            2662..3237
                     /locus_tag="VF453_12225"
     CDS             2662..3237
                     /locus_tag="VF453_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7688469.1"
                     /translation="MFHTPIESRAMSKQPLQFPAPMDLCRDQAPATMDCQGLRLAAEV
                     RRSSSGQAFRPAFAAGDWRVFASFCVARRLPADHRVLIPGRPDRTLRFVVEGSLWQES
                     VESTPDASGRPRLLMPGSIVGEDSLFLDAPCDLDVRTLEASLVLELTLPRHKELTAAC
                     PEIGFELLRAAGAVIAARGRTSGLRAEVATN"
BASE COUNT          443 a         1208 c         1191 g          440 t
ORIGIN      
        1 cggggcggcc gcgggcggcg cctcgaccgc ctcgggcacc ggctcgacga cgccggccgc
       61 cggcgcgggc gcgcccgtga agacgcgctt caggaactcc agaggcgccg acggcgcggg
      121 cgggggttct gcggctggtc ccgcggggct tgccgccggg tcggttttct tgcggaagaa
      181 gctgaacatg ggcggggaat tccgaggcag cggccggcgc ccgcacgggc ccggagccgt
      241 cgcgcacgcc ggcgcggaga cgtcgacagg tcgggccgcg cctgcgacgc gcaagcccgc
      301 ccgaaaattt ttcgacattg tgccgccaac cgcttcccaa ccggcagaaa agtttcgtac
      361 aatggcaggc ttggcgcttt agctcagccg gttagagcga cggaatcata atccgcaggt
      421 ccggggttcg aatccctgaa gcgccaccag atcctcgagc cgttgatgtc acgtcaacgg
      481 cttttttctt ggggcttcgg cggacgcggc gggcaacgga tctcgcggat actcgacgga
      541 catgcttccc caacgaattg gccgctacga cgtctgcgcc gagctcgggc gcgggtccac
      601 cagcatcgtc tacaagggcg tcgaccccca gagccggcgc ttcgtcgcga tcaagaccct
      661 gcagcgcgac ggctcggacg aagaggccga cgccgacacg atcctgcgcc gcttccgcaa
      721 cgaggcgcgc accatcgggc gcctgcgcca tccgggcgtc gtcgccgtcg acgagttcgg
      781 cgaggacggc ctgcacagct ggatcgcgat ggagtacgtc gagggccgca acctcgacga
      841 cgtcctgctg cgcagcccgg tgctcgggca ggagcgcgtc ctggcgatca tggacgagct
      901 cctcgacgcc ctgggctgcg tgcaccgcga gggcatctgc caccgcgacg tgaagccggc
      961 caacatcatg gtgactcccg aggggcgggt gaagctcacc gacttcgggg tggcgcggct
     1021 gcgcgacctc ggcctgacgc gcgtgtcgtc ggtgatcggg acgccggcgt tcatggcgcc
     1081 cgagcagttc accggcggcg cgctcgacca ccgcgccgac ctgttcgcct gcggcgtcct
     1141 cgcctacctg ctcctgagcg gccgccgccc gttctccggc gcgccggccg cggtgatgca
     1201 ccagatcctg cacgaggagc cgccgccgct gagcgatgcc ggcggcgggc tcgtcggatc
     1261 ggcgtacgac gacgtgatcg cgcgcgccct cgccaagcgg gcggacgcac gcttctcgtc
     1321 cgccgaggag atgcgcgccg cgctgcgcga tgcggccagg cgagccgctg aatcgccgct
     1381 cgcgacgatc gtgcggacga tggtgcagga cgtgggcagc cgccccgggc ccggcggcga
     1441 ccggccgcgc gacgagcgcg ccgatccgtc tcagcccggc ccgttcggcc ggtagcggca
     1501 ctcgaggcgg tcgccccggt agatctcgaa cggcaggtcg ccgatgcgca gctcgccgcg
     1561 ccggcgttcg accaggcgcg agagcgcggc ggcgccgggc agtgccgtca tcagctgctc
     1621 gcgggcgcgg aagatctgca tgttcaagtg cgtcgtctcc atgcccagca tgcgcgaaag
     1681 ctcggcgctg ctcagccagc cctgggcgcc cggctcgagg ccgcgctcgc gatcggccat
     1741 gcgccggcgc gcgagcgtgg cgaggcagta gtgatggctg cgctcgccca ggtcggtgcg
     1801 cgtcgcgccg tcgcgcagct ccaggcaggc gtgctcctcg tccaggctca ggcgcatgac
     1861 gagccacggc gcctcgggct cgtcggccac ctccgagacc tgggtgtcgt ccatcgccgc
     1921 cgcggcgacg aagcggtgca ggcccgcggc gccgacctgc acgcggtcgc catccttgag
     1981 cgcgcgcgtc tcgccgttgt gctccagcat ccacaacccc ggccgcgcct ggtagacgca
     2041 cagctccggc gcctcgctgc tcgggagcag gttgtgcggc gccagcacga tgacgggctg
     2101 gcgggaatcg ggcggcacga gtgcgtccac gggcgcgtcg acgtcctgca cccgccaggc
     2161 gacgccggtg ccgcggccca ggcgaacctc cgcgccgacc gcgagcggga acggctcgcc
     2221 gggcaccagc acctgtccgt cgacgaaggt gccgttgcgg ctgtggtcgg agagcatcca
     2281 gcgcccgtcg cgccagcgcg cgacggcatg catcagcgag atgccggggt cgtccagcgc
     2341 cgtgtcggcg cgcagcgcgt tgcggccgaa cacgtgctcg gcatgaagca ccacgcgttc
     2401 gccggtgcgg ggatcgaaca acgtacccat ggaagcggct ctccgcgccg gcggacgcac
     2461 cggcgcaggc cgaagtgtag tgccccggcc tcgtgccgcc gggcaggcgc gacgcacgcc
     2521 ccgggcgccg gcccgcgcct cgcggcctgc gttatgcccc gtgatggatc cgcgcggcgc
     2581 gccgtcgcaa cattcgtccc atgccacccg ccggagcgat ccgcgaagac ggtggcacca
     2641 cgacgaatcc gaggagaagc catgttccac accccgatcg aatcccgcgc catgagcaag
     2701 cagccgctgc agtttcccgc cccgatggat ctctgccgcg accaggcccc cgccacgatg
     2761 gactgccagg gcctgcgcct ggccgccgag gtgcgccgct cgtcgtcggg acaggcgttc
     2821 cggccggcgt tcgcggccgg cgactggcgc gtgttcgcga gcttctgcgt cgcccggcgc
     2881 ctgccggcgg accaccgcgt gctgatcccc ggccgccccg accgcacgct gcgcttcgtg
     2941 gtcgagggca gcctctggca ggaatcggtg gaatcgacgc cggacgcctc gggccgtccc
     3001 cgactgctga tgccgggctc gatcgtcggc gaggacagcc tcttcctgga cgcgccctgc
     3061 gacctcgacg tgcgcacgct cgaggcgtcg ctggtcctcg agctcacgct gccgcgccac
     3121 aaggagctga cggcggcctg cccggagatc ggcttcgagc tgctgcgcgc ggccggcgcg
     3181 gtgatcgccg cgcgcggccg gacgtccggg ctgcgcgccg aagtcgcgac gaactgagcc
     3241 gcccggcgcc atggcgacgc aggtgctggg cctcgtgggc ga
//
LOCUS       DASVLN010000497         9511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_187029, whole genome shotgun sequence.
ACCESSION   DASVLN010000497 DASVLN010000000
VERSION     DASVLN010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187029"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF453_12230"
     CDS             complement(<1..372)
                     /locus_tag="VF453_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX7688470.1"
                     /translation="MSFVPRTTAVLRASVACLGGLLCASAAHAVDWTGYMRGGPAATT
                     ESGHSRQCYGIGELKYRLGNECDFYGEFQLAQAMKVDGVDVNAVLMTNYYSPATESDK
                     SSNPADNFGVEQAYVEMKGVDI"
     gene            complement(644..1843)
                     /gene="mmsB"
                     /locus_tag="VF453_12235"
     CDS             complement(644..1843)
                     /gene="mmsB"
                     /locus_tag="VF453_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HEX7688471.1"
                     /translation="MDDKMTTPAAATPERRAYAGFLKNNVREYGMLLSLVAIMVFFQF
                     MTNGTLLRPLNLTNLILQNSYIVIMALGMLLVIVAGHIDLSVGSVSGFIGALAAVLMV
                     NYNWNFVAASAVCLVAGGVIGAAQGAFVAWLKIPSFIVTLGGMLIFKGLALALLDGQS
                     IGPFPDTFQLLSSGFIPDFLNGETLRLSSLAIGVLVALALFVVKLRERASHVKHGMEE
                     EPGAFFLAKQLAFAAVIVWFSWLLASYKGLPNVLVVMFALMLVYDFVARRTTIGRRIY
                     ALGGNAKAARLSGIKTERLAFLTFVNMGVLSALAGLVFAARLNTATPKAGLGFELDVI
                     AACFIGGASASGGVGKVLGAVIGAFVMGVMNNGMSIIGIGIDYQQVIKGIVLLAAVCV
                     DVYNKNK"
     gene            complement(1866..3455)
                     /gene="mmsA"
                     /locus_tag="VF453_12240"
     CDS             complement(1866..3455)
                     /gene="mmsA"
                     /locus_tag="VF453_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7688472.1"
                     /translation="MSAAESVLDRRMATTEAAGLILEMRGIRKTFPGVVALDNVDLAV
                     RAGEIHAIVGENGAGKSTLMKVLSGVYPHGSYSGEILFDGHERRFTGIADSEELGIII
                     IHQELALVPLLSISENIFLGNEPARRGLIDWAEAHRRTRELLAKVGLRESPSTLVTNL
                     GVGKQQLVEIAKALAKRVRLLILDEPTASLNEHDSQALLDLLLELKAQGIACILISHK
                     LNEISRVADSITVLRDGASVQSMDCRAGPVSEDQIIRAMVGRELADRYPPRARMAPDA
                     GEVVLEVRDWHVQHPIHHDREVVKGVSLKVKRGEIVGIAGLMGAGRTELAMSVFGGAW
                     GHGIRGEVLMNGRPIDVSTITKAIRHGIAYVTEDRKSLGLLLDDDIRHNISLANLKAV
                     SDKGVIDGGAEFAAAADYRRRLNIRCPDVLQHTVNLSGGNQQKVVLGKWLFAKPELLI
                     LDEPTRGIDVGAKFEIYSIMAALADEGKCILMISSEMPELLGMCDRIYVMNEGRFVGE
                     FASADASQEKIMRAIVKQGLI"
     gene            complement(3533..4576)
                     /gene="chvE"
                     /locus_tag="VF453_12245"
     CDS             complement(3533..4576)
                     /gene="chvE"
                     /locus_tag="VF453_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7688473.1"
                     /translation="MLAAAAIGVAALGTAQAQDKGTVGIAMPTKSSARWIADGDNMVK
                     TLQARGYKTDLQYAEDDIPNQLAQIENMVTKGDKVLVIASIDGTTLSNVLQKAADKGV
                     KVIAYDRLIKGSKNVDYYATFDNFQVGVLQATSIVDALKLKTAKGPFNIELFGGSPDD
                     NNAFFFYNGAMSVLKPYIDSGKLVVRSKQMGMDKVSTLRWDGAVAQARMDNLLSAYYT
                     NAHVDAVLSPYDGLSIGIISSLKGVGYGTPKLPMPVVTGQDAEIPSVKSILAGEQTTT
                     IFKDTRELAKVTAGMVDAVLGGKQPEINDTKTYNNGVKIVPSYLLKPVLVDKSNWHHV
                     LVDSGYYKESQIK"
     gene            4841..5818
                     /locus_tag="VF453_12250"
     CDS             4841..5818
                     /locus_tag="VF453_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688474.1"
                     /translation="MSTTTDTHWFIRARLKTRQLLLLVAMEEEGNIHGAAEILNMTQP
                     AASKLLKDLEDMLEVRLFDRLPRGVRPTWYGETMIRHARMALASLSQAHDEIDALKAG
                     RFGSVSIGAITAPAITLLPDTVAQVKAAHPSLRVNVQVETSDVLIERLNQGKLDMVVG
                     RLFARHDKSDLRYEPLVEEPVAAVVRPGHPLIERSSLKLRELQGHGWIVPPGGSVLRH
                     RFELMFQEDGLPGPTDLIETTALLFMTKMLEQSDLIGIVASDVALYYQDHGLVSILPV
                     ELPCKMDAFGLITRTDRLLAPAAKVMLRALKATASEVYGAKLDVEAPDA"
     gene            complement(5822..7177)
                     /locus_tag="VF453_12255"
     CDS             complement(5822..7177)
                     /locus_tag="VF453_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7688475.1"
                     /translation="MPRNAPARKKYRPPASRAQGVTMERALEIAFERHRAGNLDDAEP
                     IYAAVLDAQPDHVDVLSHMGILKYQRGQHDAAFALLERATTLAPGLSKPWNNLGNVLV
                     QLGRHEDAVAAFERSLALAESPEAHVSLARVWRLRGDHAASERACRRALELAPDAGLA
                     WHHLSLALLEQGAVEEGFQASLKAMQRMSPSERRRDFYARALLLSGERERAAEIYRAW
                     LAEEPDNEIARHHLAACLGRNTPARASDGYVEQVFDRFAESFDANLARLGYRGPELVA
                     EALDAVLPPPAGRLVVADLGCGTGLCGPLLRAQARTLVGVDLSQAMLDKAAARGVYDT
                     LHKDELVRFLDARRGAFDILACADTLLYFGDLRDAARAAGQALRPGGRLAFTLEAAAE
                     GAPGGYVLGTSGRYAHAAAYVRRVMADAGLEVLSMADATLRREAGLPVRGWVVAAARP
                     A"
     gene            complement(7262..8698)
                     /locus_tag="VF453_12260"
     CDS             complement(7262..8698)
                     /locus_tag="VF453_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HEX7688476.1"
                     /translation="MAAPDVPASAASVAGAASAVASSAAGLGAHDERVLLVAALGIAL
                     LVLLIVWLRLHAFLALTIGALFVGVGSGLPLDKITASYENGVGGVLGYVGVLIALGAM
                     LGKLLADSGGADKVVDTLLRGREATLPWKMAAVAAIIGIPMFFEIGLVLLIPVVMLAV
                     HRSKGPAMRLGIPALAGLSVLHGFIPPHPGPLAAISILHADVGLTLALGVVVAIPTVI
                     VAGPLFGILAARLVPVGAAGAGLAVAGGQGGDAATRPPHSPGFGTTLATLASPIVLML
                     AKAGADIWLAKGNGLRVVLDFVGDPVFALLVAVLLAMVTFGTALGVSIEAVGKTLSAS
                     LAPVVGVMLIVGAGGGFKQALVDGGAGAAIAKLALAAGIPVLVLGWAVAALIRLATGS
                     ATVAIVTAAGIVAPLAAGLAPAHLSLLVLSVGAGSLIFSHVNDAGFWLVKEYFGLSVG
                     ETLKTWSLMETVIAAMGLAVVLALSVAV"
     gene            complement(8685..9263)
                     /locus_tag="VF453_12265"
     CDS             complement(8685..9263)
                     /locus_tag="VF453_12265"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HEX7688477.1"
                     /translation="MTPPSTAADLSAPEPLVLVLMGVAGSGKTTVAAILAERLGWAFE
                     EGDALHPPENVEKMRAGHPLDDADRAPWLARVADWIAGRLDAGACGVITCSALKRRYR
                     EAIARGRSGVLFVHLSGSRDEIADRLAARQGHFMPPSLLASQLETLEVPGPDEPAIRV
                     DVGPPPHAIADTVVAALLQRGLLREGAAHGRA"
BASE COUNT         1452 a         3358 c         3321 g         1380 t
ORIGIN      
        1 gatgtccacg cccttcatct cgacgtaggc ctgctcgacg ccgaagttgt cggcggggtt
       61 ggaggacttg tcggactcgg tggcggggct gtagtagttg gtcatcagca cggcgttgac
      121 gtccacgccg tcgaccttca tcgcctgcgc gagctggaac tcgccgtaga agtcgcactc
      181 gttgcccagg cggtacttga gctcgccgat gccgtagcac tggcgcgagt ggccggattc
      241 ggtggtggcg gcggggccgc cgcgcatgta gccggtccag tcgacggcgt gggcggccga
      301 ggcgcacagc aggccgccga ggcaggcgac gctcgcgcgg aggacggccg tggtgcgggg
      361 gacgaagctc atgggtgggt ctccttgtga tgatgtgtcg ggcaggacgt ccgccggccg
      421 ctcggggcgg gcaaggcttc gccctgcacg ctggtccttc gcggcgtccg acggttggaa
      481 agacggcccg cgccctcttc gcgcacgcgg taccggtggg cgcctggcta tggcgccgga
      541 cgacgcgcct cgtgggcgcc gcatgccctg aaccttatac ggttgcaggt atgcatggtg
      601 ttcctcttta tctctgttgc cgcgagccga caggccgcgg ccgctacttg ttcttgttgt
      661 agacgtcgac gcagacggcg gccaggagca cgatgccctt gatcacctgc tggtagtcga
      721 tgccgatgcc gatgatggac atgccgttgt tcatcacgcc catcacgaac gcgccgatga
      781 ccgcgcccag caccttgccg acgccgccgg aagccgacgc cccgccgatg aagcaggccg
      841 cgatcacgtc gagctcgaag ccgaggcccg ccttgggcgt ggcggtgttc aggcgcgccg
      901 cgaagaccag ccccgcgagc gccgacagca cgcccatgtt gacgaaggtg aggaacgcca
      961 ggcgctcggt cttgatgccg gacagccgag ccgccttggc gttgccgccc agcgcgtaga
     1021 tgcgccggcc gatcgtggtg cggcgcgcga cgaagtcgta gaccagcatc agcgcgaaca
     1081 tcacgaccag cacgttgggc agccccttgt acgacgcgag cagccacgag aaccacacga
     1141 tcaccgccgc gaacgcgagc tgcttggcga ggaagaacgc gcccggctcc tcctccatgc
     1201 cgtgcttcac gtgcgacgcg cgctcgcgca gcttgacgac gaacagggcg agcgcgacca
     1261 gcacgccgat cgccagcgag ctcaggcgca gcgtctcgcc gttgaggaag tcgggaatga
     1321 agcccgagct caggagctgg aacgtgtcgg ggaacggccc gatcgactgg ccgtccagca
     1381 gcgccagcgc aagccccttg aagatcagca tgccgcccag cgtgacgatg aacgaaggga
     1441 tcttcagcca cgcgacgaac gcgccctgcg ccgcgccgat cacgccgccc gccacgaggc
     1501 agacggcgct cgcggccacg aagttccagt tgtagttcac catcagcacc gccgccagcg
     1561 cgccgatgaa gcccgacacc gaacccaccg agaggtcgat gtgcccggcc acgatcacca
     1621 gcagcatgcc cagcgccatg atgacgatgt agctgttctg caggatcagg ttcgtgaggt
     1681 tcagcggccg gagcagcgtg ccgttcgtca tgaactggaa gaacaccatg atcgccacga
     1741 gcgacagcag catgccgtac tcgcgcacgt tgttcttcag gaagccggcg taggcgcggc
     1801 gctcgggcgt cgcggcggcg ggcgtggtca tcttgtcgtc catggtggtc atcgaccgtc
     1861 tcttctcaaa tgaggccctg cttcacgatc gcgcgcatga tcttctcctg cgacgcgtcg
     1921 gcggaggcga actcgcccac gaagcggcct tcgttcatca cgtagatgcg gtcgcacatg
     1981 cccagcagct cgggcatctc ggacgagatc atcaggatgc acttgccctc gtcggccagc
     2041 gccgccatga tcgagtagat ctcgaacttg gcgccgacgt cgatgccgcg cgtgggctcg
     2101 tccaggatca gcagctcggg cttggcgaac agccacttgc cgagcacgac cttctgctgg
     2161 ttgccgcccg acaggttgac cgtgtgctgc agcacgtccg ggcagcggat gttcagcctg
     2221 cggcgatagt cggccgcggc cgcgaactcg gcgccgccgt cgatcacgcc cttgtccgag
     2281 accgccttca ggttggccag gctgatgttg tggcggatgt cgtcgtccag cagcagcccg
     2341 aggctcttgc gatcctcggt gacgtaggcg atgccgtggc ggatggcctt ggtgatcgtc
     2401 gaaacgtcga tcggccggcc gttcatcagc acctcgccgc ggatgccgtg gccccaggcg
     2461 ccgccgaaca cgctcatcgc gagctcggtg cgccccgcac ccatcaggcc cgcgatgccg
     2521 acgatctcgc cgcgcttgac cttcaggctg acgcccttga cgacctcgcg atcgtggtgg
     2581 atcgggtgct gcacgtgcca gtcgcgcacc tcgaggacca cctcgcccgc atcgggcgcc
     2641 atgcgcgcgc gcggcgggta gcggtccgcg agctcgcggc ccaccatcgc gcggatgatt
     2701 tggtcctcgc tcaccggccc ggcgcggcag tccatcgact gcaccgacgc gccgtcgcgc
     2761 agcaccgtga tcgagtcggc cacgcgcgag atctcgttga gcttgtgcga gatcaggatg
     2821 cacgcgatgc cctgcgcctt cagctcgagc agcaggtcga gcagcgcctg gctgtcgtgc
     2881 tcgttcaggc tcgcggtcgg ctcgtcgagg atcagcaacc gcacccgctt ggcgagcgcc
     2941 ttggcgatct ccacgagctg ctgcttgccc acgccgaggt tggtgacgag cgtggacggc
     3001 gactccctca ggcccacctt ggcgagcagt tcgcgggtgc gccggtgcgc ctcggcccag
     3061 tcgatcaggc cccggcgcgc cggctcgttg ccgaggaaga tgttctccga gatcgacagc
     3121 agcggcacca gggcgagctc ctggtggatg atgatgatgc cgagctcctc gctgtcggcg
     3181 atgcccgtga atcggcgctc atggccgtcg aacaggatct cgccggagta gctgccgtgc
     3241 gggtacacgc cgctcagcac cttcatcagg gtcgacttgc cggcgccgtt ctcgcccacg
     3301 atagcgtgga tctcgccagc gcgcaccgcc aggtcgacgt tgtccagtgc gaccacgccg
     3361 gggaacgtct tgcggatgcc gcgcatctcc aggatcaggc cggcggcctc ggtcgtggcc
     3421 atgcgcctgt cgaggacgga ttcagcagcg ctcatgaacg aagggactgg ttggatttca
     3481 agcaaagggc gaaagcgccc ggccggcgac ccgccggccg gaggccgtca tctcacttga
     3541 tctgcgactc cttgtagtag ccgctgtcga cgagcacgtg gtgccagttc gacttgtcga
     3601 ccagcaccgg cttgagcagg tacgacggca cgatcttcac gccgttgttg taggtcttgg
     3661 tgtcgttgat ctcgggctgc ttgccgccca gcaccgcgtc gaccatgccg gcggtgacct
     3721 tggcaagttc gcgcgtgtcc ttgaagatcg tggtggtctg ctcgcccgcg aggatggact
     3781 tgaccgacgg gatctcggcg tcctggccgg tcacgacggg catcggcagc ttcggggtgc
     3841 cgtagcccac gcccttgagc gaagagatga tgccgatcga caggccgtcg tacggcgaga
     3901 gcaccgcgtc gacgtgggcg ttggtgtagt aggccgacag caggttgtcc atgcgggcct
     3961 gggcgaccgc gccgtcccag cgcagcgtcg agaccttgtc catgcccatc tgcttggagc
     4021 gcaccaccag cttgccggaa tcgatgtagg gcttgagcac cgacatcgcg ccgttgtaga
     4081 agaagaacgc gttgttgtcg tccggggagc cgccgaacag ctcgatgttg aacgggccct
     4141 tggccgtctt cagcttgagc gcgtcgacga tggaagtcgc ctgcagcacg ccgacctgga
     4201 agttgtcgaa ggtggcgtag tagtcgacgt tcttcgaacc cttgatcagg cggtcgtagg
     4261 cgatgacctt gacgcccttg tcggccgcct tctgcagcac gttggacagg gtcgtgccgt
     4321 cgatcgaggc gatcaccagg accttgtcgc ccttggtgac catgttctcg atctgcgcga
     4381 gctggttggg gatgtcgtcc tcggcgtact ggaggtcggt cttgtagccg cgcgcctgca
     4441 gcgtcttgac catgttgtcg ccgtcggcga tccagcgcgc ggaggacttg gtcggcatcg
     4501 cgatgcccac ggtgcccttg tcctgcgcct gggccgtgcc cagggctgcc acgccgatgg
     4561 ccgccgcggc cagcatcgtc ttgaggaatg tcacttggtc gtctccagaa gtcggccggc
     4621 ggtcgtgccg cgcggctcgg gttttagaac gggtggatcc gcatcgagga cggctcccac
     4681 gcgccgcccg ccgcgtgcgg gcgagccgcc cgcgccgggt ctcgatgggc aggatggtac
     4741 gaacggcaga gataacttcc taatagattt ttggtgtctt gccatataca ttcgaacatc
     4801 gctcatcgcc gttcgcccgc ccgcgagccc gcccaccgcc atgtccacca ccaccgacac
     4861 ccactggttc atccgtgccc gcctgaagac gcgccagctg ctgctgctcg tggcgatgga
     4921 ggaggagggc aatatccacg gcgcggccga gatcctgaac atgacgcagc cggcggcgtc
     4981 gaagctgctg aaggatctcg aggacatgct cgaagtgcgc ctgttcgacc gcctgccccg
     5041 tggcgtgcgc cccacgtggt acggggaaac catgatccgc catgcccgca tggcgctcgc
     5101 cagcctgagc caggcgcacg acgagatcga cgccctgaag gcgggccgct tcggctcggt
     5161 cagcatcggc gcgatcacgg cgcccgcgat cacgctgctg cccgacaccg tcgcccaggt
     5221 gaaggcggcg catccgtcgc tgcgggtgaa cgtgcaggtc gagaccagcg acgtcctgat
     5281 cgagcgcctg aaccagggca agctggacat ggtcgtcggc cgcctgttcg cacgccacga
     5341 caagagcgac ctgcgctacg agccgctggt ggaggagccg gtcgccgccg tcgtgcgtcc
     5401 agggcatccg ctgatcgagc gctccagcct gaagctgcgc gagctccagg gccacggctg
     5461 gatcgtgccg cccggcggca gcgtgctgcg ccatcgcttc gagctgatgt tccaggagga
     5521 cggcctgcct gggccgacgg acctgatcga gacgacggcg ctgctgttca tgaccaagat
     5581 gctggagcag agcgacctga tcggcatcgt cgcctccgac gtcgcgctgt actaccagga
     5641 tcacggcctc gtctcgatcc tgccggtcga gctgccctgc aagatggacg ccttcggcct
     5701 catcacgcgc accgaccgcc tgctcgcgcc cgcggccaag gtcatgctgc gcgcgctgaa
     5761 ggcgacggcc agcgaggtct acggggcgaa gctggacgtc gaggccccgg acgcgtaagc
     5821 cctacgcggg cctcgcggcg gcgacgaccc agccgcgcac cggcaggccg gcctcgcgcc
     5881 gcagcgtggc atccgccatc gacagcacct cgagccccgc gtcggccatc acccgtcgga
     5941 cgtaggcggc cgcgtgcgcg tatcggccgc tcgtgcccag cacgtagcct ccgggcgcgc
     6001 cctcggccgc ggcttcgagg gtgaacgcca agcggccgcc gggccgcagc gcctggccgg
     6061 cggcgcgcgc cgcgtcgcgc aggtcgccga agtagagcag cgtgtcggcg caggccagga
     6121 tgtcgaacgc accgcggcgc gcgtcgagga agcggacgag ctcgtccttg tgcagggtgt
     6181 cgtacacgcc ccgcgcggcc gccttgtcga gcatcgcctg cgacaggtcg acgcccacca
     6241 gcgtccgcgc ctgcgcgcgc agcagcggcc cgcacaggcc ggtgccgcaa ccgaggtcgg
     6301 cgacgacgag ccggcccgcg ggcggcggca gcacggcgtc cagggcctcg gcgacgagct
     6361 cgggcccgcg gtagccgagc cgcgcgaggt tggcgtcgaa gctctcggcg aagcggtcga
     6421 agacctgctc gacgtagccg tcgctcgcgc gggccggcgt gttccgcccc aggcaggccg
     6481 ccagatggtg acgcgcgatc tcgttgtcgg gctcctcggc gagccacgcg cggtagatct
     6541 cggccgcccg ctcgcgctcg ccggacaaca gcagcgcgcg ggcgtagaag tcgcgccgac
     6601 gctcgctcgg cgacatccgt tgcatcgcct tcaacgacgc ctggaagccc tcctccacgg
     6661 cgccctgctc gagcagcgcg agcgacaggt ggtgccaggc cagcccggcg tcgggcgcga
     6721 gctcgagggc gcggcggcac gcgcgctcgc tcgcggcgtg gtcgccccga agccgccaga
     6781 cgcgcgccag gctcacgtgc gcctcgggac tctcggcgag cgccaggctg cgctcgaacg
     6841 cggccaccgc gtcctcgtgg cggcccagct gcaccagcac gttgcccagg ttgttccagg
     6901 gcttggacag ccccggcgcg agcgtcgtgg cgcgctccag cagcgcgaac gccgcgtcgt
     6961 gctggccgcg ctggtacttc aggatgccca tgtggctgag gacgtcgaca tggtccggct
     7021 gcgcgtcgag caccgccgcg tagatcggct cggcgtcgtc gaggttgccg gcgcggtggc
     7081 gctcgaaggc gatctccagc gcgcgctcca tcgtgacgcc ctgcgcgcgg gatgcgggag
     7141 ggcggtactt cttgcgggcc ggggcgttgc gcggcacggg acgggctcct gcgatcgacg
     7201 atcgacgcgc actatgccgc gaccgtgcct ccgcggcgat cctccatcgg ggggacgccc
     7261 gtcagacggc gaccgacagc gcgagcacca ccgcgagtcc catggccgcg atcacggtct
     7321 ccatcagcga ccaggtcttg agcgtctcgc ccaccgacag accgaagtac tccttgacga
     7381 gccagaagcc ggcgtcgttg acgtgggaga agatcagcga gcccgccccc accgacagga
     7441 ccagcagcga caggtgcgcc ggggcgagcc ccgcggccag cggcgcgacg atgcccgccg
     7501 ccgtgacgat ggccacggtg gccgagcccg tcgcgagccg gatcagcgcc gccaccgccc
     7561 agccgagcac cagcaccggg atgcccgcgg ccagcgccag cttcgcgatc gccgcgccgg
     7621 ccccgccgtc gacgagcgcc tgcttgaagc cgccgcccgc gccgacgatc agcatcacgc
     7681 ccacgaccgg cgcgaggctc gcgctgagcg tcttgcccac cgcctcgatc gacacgccga
     7741 gcgccgtgcc gaacgtgacc atcgccagca gcaccgcgac aagcagcgcg aagaccggat
     7801 cgcccacgaa atcgagcacg acgcgcaggc cgttgccctt ggcgagccag atgtccgcgc
     7861 ccgccttggc cagcatcagc acgatgggcg aggcgagcgt ggcgagggtg gtcccgaagc
     7921 cggggctgtg cggggggcgc gtcgcggcgt cgccgccctg ccccccggcg accgccaggc
     7981 ccgcgccggc ggcgccgacg ggcaccagcc gcgccgcgag gatcccgaac agcggccccg
     8041 cgacgatcac ggtcggtatc gccacgacga cgcccagggc cagcgtcagg ccgacatcgg
     8101 catgcaggat cgagatcgcg gccagcggcc ccggatgcgg cgggatgaag ccgtgcagca
     8161 ccgacagccc ggccagcgcc gggatgccca gccgcatcgc cggccccttc gagcggtgca
     8221 ccgccagcat cacgaccggg atcagcagca cgaggccgat ctcgaagaac atcgggatgc
     8281 cgatgatcgc ggccaccgcg gccatcttcc agggcaaggt cgcctcgcgg ccgcgcagca
     8341 gcgtgtcgac gaccttgtcg gcgccgcccg aatccgcgag cagcttgccc agcatcgcgc
     8401 ccagggcgat cagcacgccg acgtagccca gcacgccgcc gaccccgttc tcgtacgacg
     8461 ccgtgatctt gtcgagcggc aggcccgagc ccacgcccac gaacagcgcg ccgatggtca
     8521 gcgcgaggaa ggcatgcagc cgcagccaca cgatgagcag cacgagcagc gcgatgccca
     8581 gcgccgcgac gagcagcacg cgctcgtcgt gcgcgcccag cccggccgcg gacgacgcca
     8641 ccgcgctggc ggctccggcc acgctcgcag cgctcgccgg cacatcaggc gcggccatgg
     8701 gcagcccctt cgcgaagcag gccgcgctgc aggagcgccg cgaccaccgt gtcggcgatc
     8761 gcgtgcggcg gcgggccgac gtcgacgcgg atcgccggct cgtcggggcc ggggacctcg
     8821 agcgtctcga gctggctggc gagcaggctc ggcggcatga agtggccctg gcgcgcggcc
     8881 aggcggtcgg cgatctcgtc gcgcgagccg gacaggtgga cgaacagcac gccgctgcgg
     8941 ccgcgcgcga tcgcctcgcg gtagcgccgc ttgagcgccg agcaggtgat cacgccgcag
     9001 gcgccggcat cgagccggcc tgcgatccag tcggcgacgc gcgcgagcca gggcgcgcgg
     9061 tcggcgtcgt ccagcggatg gccggcgcgc atcttctcga cgttctcggg cggatgcagc
     9121 gcgtcgccct cctcgaacgc ccagccgagg cgctcggcca ggatcgcggc gacggtggtc
     9181 ttgccgctgc ccgcgacccc catcaggacg agcaccagcg gctcgggggc ggacaggtcg
     9241 gcggcggtcg aaggcggggt catcggcgag tcttcagcgg tggcggcgac gcgcgactgt
     9301 cgcacggatc gcgatcccgg gccagcgccg gggacgccga ccggcggccg acgaccgtgt
     9361 caggcggcgt agcggaacgt ggccatcgcc agcaccgtca gcacgagcgc cccggccgcg
     9421 atgcgctgca ggacgcgcag gcgcatctcc gacgcgaagc ccgccgagcg cgccgacgcc
     9481 gccgccgcag cctgcagcgc gagcgccagc a
//
LOCUS       DASVLN010000498         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_456661, whole genome shotgun sequence.
ACCESSION   DASVLN010000498 DASVLN010000000
VERSION     DASVLN010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456661"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VF453_12270"
     CDS             <1..763
                     /locus_tag="VF453_12270"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX7688478.1"
                     /translation="AQGGTDGESLAGALARLPTELRRNRRAVAKSDFAELALQTPAVR
                     LARAECMPLFHAPSRSLKPGCVSVVVWPAQDAQHPNAPLPDAWELAQACSWLDRWRLV
                     TTELYVIPPSYRRIAIAVSVKVQAGYGLDAVRDWLDILLRQYLAPVPPYGPDGTGWPL
                     GRRVIARELEGVAMQVEGVDYIQELRLDFSRQDAGGSETWAAIDVLEIAGWEVPAVAA
                     VVVVDDATPLPAPGTDLQPPPSRPAVPVPIPVDEC"
     gene            778..2874
                     /locus_tag="VF453_12275"
     CDS             778..2874
                     /locus_tag="VF453_12275"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX7688479.1"
                     /translation="MRLVPDTGTRMLRGPAHWQACALHDTVLVDDVVMLASDPAPVPP
                     GLGNTPFTGGFAGLAFDQRCRLFHPRPEDGTIETVLWARTSSLGVHDAPAAPFAIAAP
                     GSGGALPRRPLALACDDRAFLYVADPDARTVWLVDTWQQMVSRAIAFSAAPLDLSACG
                     DAVFALLDDGSTWQLTPCDEPRRMPWPARAGADRLVVTTSAAGGWLAWVLQRAGQVDA
                     QLWALHLDRGLAAPFCTDVVAEGEDANHGALVTLAQRPGEEFLRMRLAGSQPAAQPSL
                     SAPNYDGRGITLAPDGRISYWTAKGLRQAAPARMHYRPEGRVFGFALDSGFDQNRWGQ
                     VCIEACVPAGTQLRMAAFTRDALDFVDPIDGLGAGTTIPVLSQATWAAFDGDDQALSR
                     DPSQRPLSPVPADGFAFHDAPVVAGLGRYLWLVFEFSGTRSKSPRLRAVDVEYPGHPL
                     LQSLPRTLWREPAAQGFLFRLLMPMAAMLAEWRDVSATRHRLLDARISPTEALGWLAG
                     FVGLVLDPCWPQAVQRRMIAQAAPLFRTRGTLASLQAMLRTLTGAEVVILEDFRLRGG
                     GVVGNAQAQASQAVLGYGYRVGGLIGEPGAAPVADAPGAEFDDFAHRFSVTIVAALDD
                     AQLACARRLVEMHKPAHTGFTLCTACAGIRAGVGDHVGLSTVVGKSAGFEVAIVGDSA
                     LDQGYLLGRPPIQEGA"
BASE COUNT          355 a         1170 c         1105 g          421 t
ORIGIN      
        1 ggcgcagggc ggcacggacg gcgagagcct ggccggcgcg ctcgcccgcc tgcccaccga
       61 gctgcgccgc aaccgccgcg ccgtggcgaa atcggacttc gccgagctcg ccctgcagac
      121 gccggccgtc cgcctcgcgc gcgccgaatg catgccgctg ttccacgcgc cgagccgcag
      181 cctcaagccg ggctgcgtca gcgtggtcgt gtggccggcg caggatgcgc agcatccgaa
      241 tgcgccgctg cccgacgcct gggagctcgc gcaggcctgc agctggctcg accggtggcg
      301 gctcgtcacc accgagctct acgtgatccc gccgagctat cgccgcatcg cgatcgccgt
      361 ctcggtgaag gtgcaggccg gctacggcct ggacgccgtg cgcgactggc tcgacatcct
      421 gctgcgtcaa tacctggccc ccgtaccgcc ctacggaccg gacggcaccg gctggccgct
      481 cggccgccgc gtcatcgcgc gcgagctcga gggcgtggcg atgcaggtcg agggcgtcga
      541 ttacatccag gagctgcgcc tggacttctc gcgccaggat gccggcggga gcgagacctg
      601 ggccgcgatc gacgtgctcg agatcgccgg ctgggaggtg ccggcggtcg ccgccgtcgt
      661 cgtggtcgac gacgcgacgc cgctgcccgc tccgggcacc gacctgcagc cgccgccatc
      721 ccggccggcc gtgccggtgc ccatccccgt cgacgagtgc tgacgcaggc ggccgcgatg
      781 cgactcgtcc ccgataccgg tacccgcatg ctgcgcggcc ccgcgcactg gcaggcgtgc
      841 gcattgcacg acacggtgct ggtggacgac gtcgtcatgc tggcgagcga tcccgcgccc
      901 gtgccgccag gcctgggcaa cacgccgttc acgggcgggt tcgcgggcct cgcgttcgat
      961 cagcgctgcc ggctgttcca tccgcggccc gaggacggca cgatcgagac cgtgctctgg
     1021 gcacggacca gcagcctggg cgtgcacgac gcgcccgccg cgccgttcgc gatcgccgcg
     1081 cccggatccg gcggcgcgct gccgcggcgg ccgctggcgc tcgcctgcga cgatcgcgcc
     1141 ttcctctacg tcgcggaccc ggacgcgcga accgtgtggc tggtcgacac ctggcagcag
     1201 atggtctcgc gcgcgatcgc gttcagcgca gcgccgctgg acctctcggc ctgcggcgac
     1261 gcggtcttcg cgctgctgga cgacggcagc acgtggcagc tcacgccctg cgacgagccg
     1321 cgccgcatgc cctggcccgc gcgggccggc gcggatcgcc tcgtcgtcac cacgtccgcg
     1381 gccggcggct ggctcgcctg ggtgctgcag cgggccgggc aggtcgacgc tcagctgtgg
     1441 gcgctgcacc tcgaccgcgg cctcgcggcg ccgttctgca ccgatgtcgt cgccgagggc
     1501 gaggatgcga accacggcgc gctcgtcacc ctggcgcagc gcccgggcga ggaattcctg
     1561 cgcatgcggc tcgcgggcag ccagcccgcg gcgcagccct cgctgtctgc gccgaactac
     1621 gacggccgcg gcatcacgct cgcgcccgac ggccgcatct cgtactggac ggccaagggc
     1681 ctgcgccagg ccgctcccgc gaggatgcac taccgccccg agggccgtgt gttcggcttc
     1741 gcgctggact ccggcttcga ccagaaccgc tggggccagg tatgcatcga ggcgtgcgtc
     1801 ccggcgggca cgcagctgcg aatggccgcg ttcacgcgcg acgccctcga cttcgtcgat
     1861 ccgatcgacg gcctgggcgc cggcacgacg atcccggtgc tgtcgcaggc gacgtgggcc
     1921 gcgttcgacg gcgacgacca ggcgctgtcc cgcgacccgt cgcagcgccc gctctcgccg
     1981 gtgcccgcgg acggcttcgc gttccacgac gcaccggtcg tcgcgggtct cggccgatac
     2041 ctctggctcg tattcgaatt cagcggcacg cgcagcaagt cgccgcggct gcgcgccgtc
     2101 gacgtcgagt atccgggcca tccgctgctg cagtcgctgc cgcgcacgct ctggcgcgag
     2161 cccgcggcgc aaggcttcct ctttcgactg ctgatgccga tggcggcgat gctggcggag
     2221 tggcgcgacg tctccgcgac gcgccatcgc ctgctcgacg cacgcatctc gccgaccgag
     2281 gcgctgggct ggctcgcggg cttcgtgggc ctggtgctcg acccgtgctg gccgcaggcg
     2341 gtgcagcggc ggatgatcgc ccaggccgcg ccgctgtttc gcacgcgcgg cacgctcgcg
     2401 agcctgcagg cgatgctgcg gacgctcacc ggtgccgaag tggtgatcct cgaggacttc
     2461 cgcctgcgcg gcggcggggt cgtcggcaac gcgcaggcgc aggcctcgca ggccgtgctg
     2521 ggctacggct accgcgtcgg cggcctgatc ggcgagccgg gcgccgcccc ggtggccgac
     2581 gcgcccggcg ccgagttcga cgatttcgcc caccgcttca gcgtcaccat cgtcgccgcg
     2641 ctcgacgacg cgcagctcgc ctgtgcgcgc cgcctggtcg agatgcacaa gcccgcgcac
     2701 accgggttca cgctgtgcac ggcctgcgcc ggcattcgcg cgggcgttgg cgaccatgtc
     2761 ggcctgagca cggtggtcgg caagtccgcc ggcttcgagg tggcgatcgt cggcgactcg
     2821 gcgctggacc agggctacct gctgggacgg ccgccgatac aggagggggc atgaccaccg
     2881 ggccggccgc ttcgaatggc gcccgcaccg cagtgcgcag cgcggagcgc tcctcatgag
     2941 cggcctcgcc atcgaccgcc tgcacctgcg cctgcgcagc ccccgcccgc tgccggccgg
     3001 gcgcgtggac gcctggacgg acgccgtcgc cgcgctggac gccgacgccc t
//
LOCUS       DASVLN010000499         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_347688, whole genome shotgun sequence.
ACCESSION   DASVLN010000499 DASVLN010000000
VERSION     DASVLN010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347688"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..3726
                     /locus_tag="VF453_12280"
     CDS             181..3726
                     /locus_tag="VF453_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7688480.1"
                     /translation="MWMTRVAIANPVFATMIMVALCVLGLFSYQRLGVEQMPDIAPPV
                     VFISVNYPGASPGAVETEVTKPLEDALNGIAGVKMIRSNSLEGRSETVVEFNLAADMT
                     RAVQDVRDKVAGVQAGFNRDVKQPFVARFDNDNAQPTVVLALLAHGRSDRELSLIADQ
                     TVQKRLTSVEGVARVTPLGLVTRQVRIDLDPQRLRAFGLTPADVSAAIARANTDQPVG
                     MLQARNEDSIVRVEGRIQDPKQFADIVVSRNAGGGVVLLGDLGKLVEREQEPDSLSRI
                     DGRPAVTFNIYKQQDANIVRTGAAIQEAAEAMKKTLPPGVELKTVYASSDFVAQSLDG
                     VKKTLVEGALLTIAIVFLFLHSWRSTVITGLTLPIAVIASFIAVNAFGFTLNFMTMMA
                     LSLCIGLLIDDAIVVRENIVRHVGMGQDHHTAARLGTDEIGLAVMATTFAICAVFVPV
                     AFMKGIVGKFFFPFGITVVSAVVVSLFVSFTLDPMLSSVWRDPPAATLARVWGIGHAM
                     RLVDRGMAGLHHVYERLIGWAFSGRRYRLLVPPVPAFGLGFDAQGRRLASGGRHWRFA
                     TLTPRGIVLWIAAATFAAAIALATTIGTEGVPQTDQSYTSINVTMPVGTSLQRADDKV
                     RQIEGLIGKMPEIATLSTQVGGDYGHNTAYLGIGLKPRHERKLTQAQVEDEIRKRLAS
                     VPGIQVALGNQPIYIAILGPDPTVLDQITADLAAKIQKVKGITDFSTSVKPGLPAYAV
                     RVRPDAARQLGLTASDLAASLRAYVQGDVSTYWTTPDGQQVEVQLRLPEASRRDVKQL
                     EQLPVAYSKDGTPIALASVASITPVTNPDIIKRQDLQRRQAIYAGVDQKSGRTTGDVN
                     KDVQKIIDATVLPPGYRFDVGGSAKDQDEIFGNIGSALGLALIFIYIVLASQFGSFLQ
                     PVAIMASLPLSLIGVMVSLKVWGSTINLFSLIGLIMLMGLVTKNAILLVDFANHARKA
                     GATLAEAVLSAGQIRMRPIIMTTAAMVFGMFPLALALDDGGELQAPMGRAIIGGVITS
                     TLLTLVVVPVIYAYIEQWVGFYKHRLDAKSRGAMTGAVLAGLLGAVLAMTAVPVAATA
                     VVVAAALALSVQGCLCYVRANGYAPAWAAMGLGGLPGFMILAFLRPIQDGGPPEELAM
                     TSPAAPSPDAATVSALDSQPHPAPDAEPEPDSVAA"
     gene            4159..>4365
                     /locus_tag="VF453_12285"
     CDS             4159..>4365
                     /locus_tag="VF453_12285"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX7688481.1"
                     /translation="MRPLIVLALLAASAGAQAQYKCTSSGGAVTFQQQPCPPGQKESA
                     LDVKPNGGAHAAASAPAAASATPAV"
BASE COUNT          661 a         1577 c         1490 g          637 t
ORIGIN      
        1 cgcgccgagg tgctggacgg cctggccccc gacgcgcccg tgctgggcat gcgcttcgag
       61 aacctgcgcg agggcgcgcc cgcgcgcatc gtcgccacgg cgaccacggc cacgccgacc
      121 ccgtcgcccg ccaccatcac ccgctgacgc acgccggtcc gccggccaag gagagctccc
      181 atgtggatga cgcgcgtcgc catcgccaac cccgtcttcg cgaccatgat catggtggcg
      241 ctgtgcgtgc tcggtctgtt ctcgtaccag cgcctgggcg tcgagcagat gcccgacatc
      301 gcgccgccgg tcgtcttcat cagcgtgaac tatcccggcg cgtcgcccgg cgcggtggag
      361 accgaggtca cgaagccgct ggaggacgcc ctcaacggca tcgccggcgt gaagatgatc
      421 cgctccaaca gcctggaagg gcgcagcgag acggtcgtcg agttcaacct cgccgcggac
      481 atgacgcgcg ccgtgcagga cgtgcgcgac aaggtcgccg gcgtgcaggc cggcttcaac
      541 cgcgacgtca agcagccctt cgtggcgcgc ttcgacaacg acaacgcgca gcccaccgtc
      601 gtcctcgcgc tgctggccca cgggcgcagc gaccgcgagc tgtcgctgat cgccgaccag
      661 acggtgcaga agcgcctgac gagcgtcgag ggcgtggcgc gggtcacgcc gctgggtctc
      721 gtcacgcgcc aggtgcgcat cgacctcgac ccgcagcgcc tgcgcgcctt cggcctcacg
      781 ccggcggacg tctcggccgc gatcgcgcgt gccaacaccg accagcccgt gggcatgctg
      841 caggcgcgca acgaggactc gatcgtgcgc gtcgagggcc gcatccagga tcccaagcag
      901 ttcgccgaca tcgtggtctc gcgcaacgcg ggcggcggcg tcgtgctgct gggcgacctc
      961 ggcaagctgg tcgagcgcga gcaggagccc gactcgctct cgcgcatcga cggccgcccc
     1021 gcggtcacct tcaacatcta caagcagcag gacgccaaca tcgtcaggac cggcgccgcc
     1081 atccaggaag ccgccgaggc gatgaagaag acgctgccgc cgggcgtcga gctgaagacg
     1141 gtctacgcga gcagcgactt cgtcgcccag tcgctggacg gcgtcaagaa gacgctggtc
     1201 gagggcgcgc tgctcaccat cgcgattgtc ttcctgttcc tgcacagctg gcgctcgacg
     1261 gtcatcacgg ggctgacgct gccgatcgcc gtgatcgcgt ccttcatcgc cgtcaacgcc
     1321 ttcggcttca cgctcaactt catgacgatg atggcgctga gcctgtgcat cggcctgctg
     1381 atcgacgacg cgatcgtggt gcgcgagaac atcgtgcgcc acgtcggcat gggccaggac
     1441 caccacaccg cggcgcgcct gggcaccgac gagatcgggc tggccgtgat ggcgacgacc
     1501 ttcgcgatct gcgccgtgtt cgtgccggtg gcgttcatga agggcatcgt cggcaagttc
     1561 ttcttcccct tcggcatcac ggtggtgtcg gccgtggtcg tgtcgctgtt cgtgagcttc
     1621 acgctcgacc cgatgctgtc gtccgtctgg cgcgacccgc cggcggcgac gctggcgcgc
     1681 gtctggggca tcggccacgc gatgcgcctc gtcgaccgcg gcatggcggg cctgcaccac
     1741 gtctacgagc ggctgatcgg ctgggccttc agcgggcgcc gctaccgcct gctggtgccg
     1801 ccggtgcccg ccttcggcct cggcttcgac gcccagggcc ggcgcctcgc ctcgggcggg
     1861 cggcactggc gcttcgccac gctcacgccg cgcggcatcg tgctgtggat cgccgcggcc
     1921 accttcgccg cggccatcgc gctggccacg accatcggca ccgaaggggt cccgcagacc
     1981 gaccagagct acaccagcat caacgtgacg atgcccgtgg gcaccagcct gcagcgcgcc
     2041 gacgacaagg tacgccagat cgagggcctg atcggcaaga tgcccgagat cgcgacgctg
     2101 tcgacgcagg tcggcggcga ctacggccac aacacggcct acctgggcat cgggctgaag
     2161 ccgcgccacg agcgcaagct cacgcaggcg caggtcgagg acgagatccg caagcgcctc
     2221 gcgagcgtgc cgggcatcca ggtcgcgctc ggcaaccagc cgatctacat agccatcctc
     2281 ggcccggatc cgaccgtgct ggaccagatc accgccgacc tcgcggccaa gatccagaag
     2341 gtcaagggca tcaccgactt ctcgacctcg gtcaagcccg gcctgccggc ctacgccgtg
     2401 cgcgtgcgcc cggacgccgc gcgccagctg ggcctgaccg cgagcgacct cgccgccagc
     2461 ctgcgcgcct acgtgcaggg cgacgtctcg acctactgga cgacgcccga cggccagcag
     2521 gtcgaggtgc agctgcgcct gcccgaggcg agccgccggg acgtcaagca gctcgagcag
     2581 ctgcccgtcg cctactcgaa ggacggcacg ccgatcgcgc tggcgagcgt cgcgtcgatc
     2641 acgccggtga ccaaccccga catcatcaag cgccaggacc tgcagcgccg ccaggcgatc
     2701 tacgccggcg tcgaccagaa gtccggccgc acgaccggcg acgtcaacaa ggacgtgcag
     2761 aagatcatcg acgccaccgt gctgccgccg ggctaccgct tcgacgtcgg cggctcggcc
     2821 aaggaccagg acgagatctt cggcaacatc ggcagcgcgc tgggcctcgc cctgatcttc
     2881 atctacatcg tgctggcgag ccagttcggc agcttcctgc aaccggtggc catcatggcc
     2941 tcgctgccgc tgtcgctgat cggcgtgatg gtctcgctga aggtctgggg ctcgacgatc
     3001 aacctgttct cgctgatcgg cctgatcatg ctgatggggc tggtcaccaa gaacgcgatc
     3061 ctgctggtgg acttcgccaa ccacgcgcgc aaggccggcg cgacgctggc cgaggccgtg
     3121 ctcagcgcgg gccagatccg catgcggccg atcatcatga cgaccgcggc gatggtcttc
     3181 ggcatgttcc cgctggcgct cgcgctggac gacggaggcg agctgcaggc gccgatgggc
     3241 cgcgccatca tcggcggcgt gatcacctcg acgctgctca cgctggtggt ggtgccggtg
     3301 atctacgcct acatcgagca gtgggtcggc ttctacaagc accggctgga cgcgaagtcc
     3361 cgcggcgcga tgaccggcgc cgtgctggcc ggcctcctgg gcgcggtgct cgcgatgacg
     3421 gcggtgcccg tggccgccac ggcggtcgtc gtggcggcgg ccctcgcgct gagcgtccag
     3481 ggctgcctct gctatgtgcg cgccaacggc tacgcgccgg cctgggcggc gatgggcctg
     3541 ggcggcctgc cgggcttcat gatcctcgcg ttcctgcgcc cgatccagga cggcggcccg
     3601 cccgaggaac tcgcgatgac gtccccggcg gcaccgtcgc ccgacgcggc gacggtctcg
     3661 gccctcgact cgcagccgca ccccgcgccg gacgcggagc cggagccgga ttccgtcgcc
     3721 gcctgactcg accggcgggc cgcacccggc gatcgcgcgg tgtcctgcct cgcgcccgag
     3781 cgtcgcgacg gcgcccatcg ggcccggagt cgaatgaggt cgactcgcgg gtgaatgaat
     3841 tcgatcggcc gtggaacgaa ttcgtcgccc gaggcaatga acgccccgtc ctggggcgtt
     3901 catgatgtcg gcctggcaat gaattcgatc cggagaccaa tgagttccat ccgccgggca
     3961 atgaattcat cgcccgatgg aattcattgg atatgcaggg gaattcattg ctcgccgaaa
     4021 tgaattcatg gccctgcgcg atgaatgaat tcgatgccgc catgaatgaa cgcacggccc
     4081 cgcggcggcg cgcgcgctcg gcgctgcgct gtcgcgaacc gggatgccgg gggcggccga
     4141 cgatgctatg gtcgcggcat gcgacctctc atcgtcctcg ccctgctggc ggcctcggcc
     4201 ggcgcccagg cccagtacaa gtgcacgtcg tcgggcggcg ccgtgacgtt ccagcagcag
     4261 ccgtgcccgc cagggcagaa ggagagcgcg ctcgacgtga agccgaacgg gggtgcacac
     4321 gcggccgcca gcgcgccggc tgccgcgtcg gcgacgccgg cggtg
//
LOCUS       DASVLN010000500         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_240186, whole genome shotgun sequence.
ACCESSION   DASVLN010000500 DASVLN010000000
VERSION     DASVLN010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240186"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VF453_12290"
     CDS             <1..1161
                     /locus_tag="VF453_12290"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX7688482.1"
                     /translation="WAATTLVPATARVELLRATSAPFAGGDDAWGAIVPEDFLDAAWT
                     PADELPGGGVCALAQTASATQLVVPDLYLPSQWAGEDVVQDPPAGGAGAEFGPCVDVV
                     AAPPAADVAPSVLTGLVLDPLTQAGLDAIAALQQRVVDVCEATQSLVALLDVPPDLSQ
                     SRIERWRAAFDSAWAAAYHPWLIPTRRHDDDEDVATNRPRRLPPSAVAAGIVARRERT
                     YDLPYGPANEVANSVIHVAEAQPPGRADVLQPLSINCFVRRQEGIALVAARTLARDGD
                     WRQLSVRRMVLMIRRALVAQTQWAVFEPNGPALQRDLVHAIQGLLRGLFRAGAFAGAT
                     EAESFFVRVLADAPRQDAGQLVIEIGVAPAEPLEFILVRLARDGDGTLTLEE"
     gene            1161..1718
                     /locus_tag="VF453_12295"
     CDS             1161..1718
                     /locus_tag="VF453_12295"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX7688483.1"
                     /translation="MAATLILPAFRFEVTLLRSADIVAGAQSPPGAPDEPPATGGTPL
                     VPGDWSGAFQECGGLEIEMDVQEYQEGGRNDGTIRRVGRAKFAPLVLKRGMFHGSEAS
                     AAAAPELWQWLQDIVNGVRPVRRYDGVVRVMSADGHVRATWTFDRGLPSKIRGPALDG
                     KSGEVAIEELTIQHEGLRLMPPESA"
     gene            1741..>2239
                     /locus_tag="VF453_12300"
     CDS             1741..>2239
                     /locus_tag="VF453_12300"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688484.1"
                     /translation="MTRLVKAQLGEISAGDNPQEVAGTLVDVQFNPTSLRVQISNRTA
                     GGQQAGSQARQRPGTGEMQVSFDLAFDTADEGTTAQGVSVLTRTAAVERFVRPRGPRA
                     GQEAPPRVVFKWGGFVVQGTMESANIDLDLFDAQGLPLRAKVSVSIRGQDPRWTYRPA
                     PAAPPA"
BASE COUNT          289 a          841 c          825 g          284 t
ORIGIN      
        1 tgggccgcga cgaccctcgt gcccgcgacg gcgcgcgtcg agctcctgcg cgcgacgagc
       61 gcccccttcg cgggcggcga cgacgcgtgg ggggcgatcg tcccggagga cttcctcgac
      121 gccgcctgga cgcccgccga cgagctgccc ggcggcggcg tctgcgcgct ggcgcagacc
      181 gcgagcgcca cccagctcgt cgtgcccgac ctctacctgc cctcgcaatg ggccggcgag
      241 gacgtggtgc aggatccgcc cgccggcggc gcgggcgcgg aattcggacc ctgcgtcgac
      301 gtcgtcgcgg ccccgccggc ggccgacgtc gcgcccagcg tgctcaccgg tctcgtcctc
      361 gatccgctga cgcaggccgg gctcgatgcc atcgccgcgc tgcagcagcg cgtcgtcgac
      421 gtctgcgagg cgacgcagtc gctggtcgcg ctgctggacg tgccgcccga cctgtcgcaa
      481 tcgcgcatcg agcgctggcg cgcggccttc gacagcgcct gggccgcggc ctaccacccg
      541 tggctgatcc cgacgcgtcg gcacgacgac gacgaggacg tcgcgacgaa ccgtccgcgc
      601 cgcctgccgc cgagcgccgt ggccgcgggc atcgtcgcgc ggcgcgagcg cacatacgac
      661 ctcccgtatg ggccggccaa cgaggtcgcc aattcggtga tccatgtcgc cgaggcgcag
      721 ccgcccggcc gcgccgacgt gctgcagccg ctgtcgatca actgcttcgt ccggcgccag
      781 gagggtatcg cgctcgtcgc ggcgcgcacg ctcgcgcgcg acggcgactg gcgccagctc
      841 tcggtgcggc ggatggtgct catgatccgc cgcgcgctcg tcgcccagac gcagtgggcg
      901 gtcttcgagc cgaacggccc cgcgctgcag cgcgacctcg tgcacgcgat ccagggcctg
      961 ctgcgcggac tgttccgcgc cggcgccttc gcgggcgcca ccgaggccga gtccttcttc
     1021 gtgcgcgtgc tcgccgacgc gccgcgccag gacgcgggcc agctcgtgat cgagatcggc
     1081 gtcgcgccgg ccgagccgct cgagttcatc ctcgtgcgcc tcgcgcgcga cggcgacggc
     1141 acgctcacgc tggaggaatg atggccgcga cgctgatcct gcccgcgttc cgcttcgagg
     1201 tcacgctgct gcgctcggcc gacatcgtcg ccggtgccca gagcccgccg ggcgccccgg
     1261 acgagccgcc ggcgaccggc ggcacgccgc tggtgcccgg cgactggagc ggggcgttcc
     1321 aggaatgcgg cggcctcgag atcgagatgg acgtgcagga ataccaggag ggcggccgca
     1381 acgatggcac gatccgccgc gtcggccgcg cgaagttcgc gccgctggtg ctcaagcgcg
     1441 gcatgttcca cggcagcgag gcctcggccg ccgccgcccc cgagctctgg cagtggctgc
     1501 aggacatcgt caacggcgtg cgcccggtac ggcgctacga cggcgtcgtg cgggtgatga
     1561 gcgccgacgg tcacgtccgt gcgacctgga cgttcgaccg cgggctgccc tcgaagatcc
     1621 ggggcccggc gctcgacggc aagtccggcg aggtggcgat cgaggaactc acgatccagc
     1681 acgagggcct gcggctcatg ccgccggaat ccgcatgagc cgcgcatacg gggtaccggc
     1741 atgaccaggc tcgtgaaggc gcagctcggc gagatctcgg ccggcgacaa cccgcaggag
     1801 gtcgcgggca cgctcgtgga cgtgcagttc aacccgacct cgctgcgcgt gcagatctcc
     1861 aaccgcaccg ccggcggcca gcaggccggt tcgcaggcgc gccagcggcc cggcacgggc
     1921 gagatgcagg tgagcttcga cctcgccttc gataccgccg acgagggcac gacggcgcag
     1981 ggcgtgagcg tgctgaccag gaccgccgcg gtcgagcgct tcgtgcggcc caggggcccg
     2041 cgcgccggcc aggaggcgcc gccgcgggtc gtcttcaaat ggggcggatt cgtcgtgcag
     2101 ggcacgatgg agagcgccaa catcgacctg gacctcttcg atgcccaggg cctgccgctg
     2161 cgcgccaagg tctcggtgtc catcagaggc caggacccgc gctggacgta ccgaccggcg
     2221 cccgccgcgc cgccggcgg
//
LOCUS       DASVLN010000501         5016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_187090, whole genome shotgun sequence.
ACCESSION   DASVLN010000501 DASVLN010000000
VERSION     DASVLN010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187090"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VF453_12305"
     CDS             <1..544
                     /locus_tag="VF453_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HEX7688485.1"
                     /translation="ATLALGPGRLPRLEPELVVAFERVPAPEAGLDELAASLAWMAPG
                     FEIVVSHLPAWEYAPAQTIADSAVHGALVVGRPLSVPRGAGAGEALARTLADARMRLR
                     DGTGRVVAEGLGANVLGDPLHALQRFLADIAATPGVRPPAPGELATTGTWSDSVPIAP
                     GETWRADFDAGLGTLALALR"
     gene            657..1970
                     /locus_tag="VF453_12310"
     CDS             657..1970
                     /locus_tag="VF453_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7688486.1"
                     /translation="MSTTSLDPQASAGGLRTAADAVYRKVSMRLLPLLFLCYIAAYLD
                     RVNVGFAKLGMQDALKLSDDVYGFGAGIFFVGYFIFEIPSNLALHRVGAGKWIARIMV
                     SWAVLSAATMFVTTPMAFYIVRFLLGVAEAGFFPGIILYLTYWYPAARRGRATSLFLT
                     AVAFAGIIGGPVSGWILHSMGGVNGWAGWQWLFLFEALPSLVLGVMAWFFLEDRVKNA
                     QWLTADERELIARDIAAEDARKSQGRLAEVVSDPQVWVMSLVYFGIVSGLYGISFWLP
                     TIIKAMGVSDPLNVGLVSAVPWAFGVVAMYLLAHSADRRGEARWHTALPCIAGAAGLE
                     ISVLFHGDVMLAMAGLTLCAMGIMAALPVSWSNPTALLAGSAAAAGIAMINSIGNLAG
                     FAIPWIFGIIKKQTGSTDGGLHMLAGLMLLSAVAVLSLRTARRDD"
     gene            2152..2673
                     /locus_tag="VF453_12315"
     CDS             2152..2673
                     /locus_tag="VF453_12315"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX7688487.1"
                     /translation="MTPATPELASAHPVVLHAPQRHLPRARAVFDDTELLLSAILPAA
                     RIEHVGASAVPGALSKGDVDVCVLLARDAFHEALGVLGEAGFTLRSRADRNADRYALA
                     APQGDLPLMVHLVEDRDAPEPLLAFRDALRRDPVLLERYNALRLEAAAQGAVAYRAAK
                     AHFIAEVLEGLGV"
     gene            complement(2670..3050)
                     /gene="speD"
                     /locus_tag="VF453_12320"
     CDS             complement(2670..3050)
                     /gene="speD"
                     /locus_tag="VF453_12320"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX7688488.1"
                     /translation="MQGLHLIADLRRCAGGVALDDLPALRALCTAAVADAGLRAVGEL
                     FHPFVDADGRPQGVTGVVLLVESHLAVHTWPELGGVTADVYVCNVGADNSARAEAVLR
                     SLVAAFRPGEARVERVRRGELAAG"
     gene            3197..4081
                     /locus_tag="VF453_12325"
     CDS             3197..4081
                     /locus_tag="VF453_12325"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HEX7688489.1"
                     /translation="MHDLAADLHSHSTVSDGTLDAAAMAERAKANGVALWALTDHDEL
                     GGLPAARAAAERVGLPFLDGIEISVTFAGHTVHIVGLGVDPGEEALRAGVAAVRAGRE
                     LRARQMADGLAKAGIRGAYEGAVKLAPNPDLVSRTHFARHLVNTGVAPDMHAVFRRYL
                     TEGKPGYVEHRWAKLGDAVRWIRGAGGVAVIAHPGRYRFTPTEEYALFTEFQAHGGEA
                     VEVVTGSHHAGDVAKYTEMALEFGLLASRGSDFHDPGESRLDLGLLPDRTALSPRLTP
                     VWSIWPELAAAAGAQGTS"
     gene            4078..>5016
                     /locus_tag="VF453_12330"
     CDS             4078..>5016
                     /locus_tag="VF453_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7688490.1"
                     /translation="MTRGPAHAHPLQGIALVLVAAACFAGLDSSTRALTQASGRWHMP
                     VLVVLTSRYTLQAVVMAIWLAPRGRAGFAAAHPRFQALRGGLLLATSLCSFFGVSLMP
                     VPEYTAINMITPLLVTVLAATVLHERVSTLRWALVAGGFAGTLVVIRPGSGAFGWVVA
                     LPVLGAIAYASFQVLTSRLAGAEDPLTTHFYTGFVGAACLLPALAAATSVQGLAWTAR
                     MDASGWGLLALGGALGTAGHLLLILALGRAGTATLMPFVYTQIGFAALIGVVAFGHWP
                     DGWALAGMAVIAACGASSAALNLRRPGAAAPAAAVDI"
BASE COUNT          602 a         1878 c         1827 g          709 t
ORIGIN      
        1 ggcgacgctc gcgctcggcc cgggacgcct gccgcggctc gagcccgagc tcgtggtcgc
       61 cttcgaacgc gtgcccgcgc ccgaggcagg cctcgacgag ctcgcggcca gcctcgcctg
      121 gatggcgccg ggcttcgaga tcgtcgtctc gcacctgccc gcctgggagt acgcgccggc
      181 ccagacgatc gccgacagcg cggtgcacgg cgccctggtc gtcggccggc cgctgtccgt
      241 gccgcgcggc gcgggcgccg gcgaggccct cgcgcgcacg ctggcggacg cgcgcatgcg
      301 cctgcgcgac ggcacgggcc gcgtggtcgc cgagggactc ggcgcgaacg tgctgggcga
      361 cccgctgcac gcgctgcagc gcttcctcgc ggacatcgcc gccacgcccg gcgtgcgccc
      421 gccggcgccg ggcgagctgg ccaccaccgg cacctggtcc gattcggtgc cgatcgcgcc
      481 gggcgagacc tggcgcgcgg acttcgacgc cggcctcggc acgctggcgc tcgcgctgcg
      541 ctgaacgccg ccgcctgcgc gttttcccgg attactgccg agccgcccgg gcggacgatc
      601 gcggctgcaa gcgccgcgcg agacggtccc atcacaacga ctggagagag cacgccatga
      661 gcacgacttc attggacccg caggcaagcg ccggcggcct gcgcaccgcc gcggacgcgg
      721 tctaccgcaa ggtgagcatg cggctgctgc cgctgctgtt cctgtgctac atcgcggcct
      781 acctcgaccg cgtcaacgtc ggcttcgcca agctgggcat gcaggacgcg ctcaagctca
      841 gcgacgacgt ctacggcttc ggcgcgggca tcttcttcgt cggctacttc atcttcgaga
      901 tcccgagcaa cctcgcgctg caccgcgtgg gcgctggcaa gtggatcgcg cgcatcatgg
      961 tctcgtgggc cgtgctgtcg gcggcgacca tgttcgtcac gacgccgatg gcgttctaca
     1021 tcgtccgctt cctgctcggg gtcgccgagg cgggcttctt cccgggcatc atcctgtacc
     1081 tcacgtactg gtacccggct gcgcggcgcg ggcgcgcgac ctcgctgttc ctcaccgcgg
     1141 tggccttcgc gggcatcatc ggggggccgg tgtcgggctg gatcctgcac tcgatgggcg
     1201 gcgtcaacgg ctgggcgggc tggcagtggc tgttcctgtt cgaggcgctg ccctcgctgg
     1261 tgctcggcgt gatggcgtgg ttcttcctcg aggaccgcgt gaagaacgcg cagtggctga
     1321 ccgcggacga acgcgagctg atcgcgcgcg acatcgccgc cgaggacgcg cgcaagtccc
     1381 agggccggct cgccgaggtc gtgtccgacc cgcaggtgtg ggtgatgtcg ctggtgtact
     1441 tcggcatcgt ctcggggctg tacggcatct cgttctggct gcccaccatc atcaaggcga
     1501 tgggcgtgag cgacccgctg aacgtcggcc tcgtcagcgc ggtgccctgg gcgttcggcg
     1561 tcgtcgcgat gtacctgctc gcgcacagcg ccgaccgccg cggcgaggcg cgctggcaca
     1621 ccgccctgcc ctgcatcgcc ggggccgcgg gcctggagat cagcgtgctg ttccacggcg
     1681 acgtgatgct ggccatggcg ggcctcacgc tgtgcgcgat gggcatcatg gcggcgctgc
     1741 cggtgtcctg gagcaacccg accgcgctgc tggccggcag cgcggccgcc gcaggcatcg
     1801 cgatgatcaa ctcgatcggc aacctcgccg gattcgcgat cccctggatc ttcgggatca
     1861 tcaagaagca gaccggctcg accgacggcg gcctgcacat gctggccggc ctgatgctgc
     1921 tgagcgccgt cgccgtgctg agcctgcgca ccgcgcgccg ggacgactga cctcgcctcg
     1981 ggcacgccgc ctgccgcgcg gcgccgtccg ggcgcggccg agcgcgcttg ccgcgccgga
     2041 tcggtcgtgc agacgtgaac ggatggacac aagtccaccg gttttggggg tctccgaccg
     2101 gatgtcgcat gcgacaaaat gcgcccaacc cgccgcaagg atgacaacaa gatgacgccg
     2161 gccacgcccg aacttgcttc cgcgcacccc gtcgtgctgc acgcgccgca gcgccacctg
     2221 ccgcgggcgc gcgcggtctt cgacgacacc gagctgctgc tgtcggccat cctgcccgcg
     2281 gcgcgcatcg agcacgtcgg cgcctcggcg gttccgggcg cgttgtccaa gggcgacgtc
     2341 gacgtctgcg tgctgctcgc acgcgacgcg ttccacgagg cgctgggcgt cctcggcgag
     2401 gcgggcttca cgctgcgcag ccgcgcggac cgcaacgccg accgctacgc gctcgccgcg
     2461 ccgcaaggcg acctgccgct gatggtgcac ctcgtcgagg accgcgacgc cccggagccg
     2521 ctgctcgcgt tccgcgacgc cctgcgccgc gacccggtgc tgctcgagcg ctacaacgcc
     2581 ctgcggctgg aagccgcggc ccagggcgcc gtcgcctacc gggcggccaa ggcgcacttc
     2641 atcgccgaag tcctggaagg cctgggcgtc taacccgcgg cgagctcgcc gcgccgcacg
     2701 cgctcgacgc gcgcctcgcc cggccggaag gcggccacga ggctgcgcag caccgcctcg
     2761 gcgcgcgccg agttgtccgc gccgacattg cagacgtaga cgtccgccgt cacgcccccg
     2821 agctcgggcc aggtgtgcac ggccaggtgc gactcgacca gcagcacgac gccggtgacg
     2881 ccctgcggcc ggccgtccgc gtcgacgaag ggatggaaca gctcgcccac cgcacgcagg
     2941 cccgcatcgg ccaccgcggc ggtgcacagc gcacgcagcg cggggaggtc gtcgagcgcg
     3001 acgccgccgg cgcagcggcg caggtcggcg atcaggtgga gtccttgcat ggcagggtgg
     3061 cggcgcgcac ccgtcgtgcg cgctgcaatg gtagggcatg gcccgcggcg aacgcgggcc
     3121 gccgcggcgt ggcggaagtc cggatcgtgc accctgcgcg cgacgaaggt cgagggggcg
     3181 ctcgtaaaat cggggcgtgc acgacctcgc cgccgacctc cacagccact cgaccgtgtc
     3241 cgacgggacg ctggacgcgg cggcgatggc cgagcgcgcg aaggccaacg gcgtcgcgct
     3301 gtgggcgctg accgaccacg acgagctcgg cggcctgccc gcggcccggg ccgccgccga
     3361 gcgggtcggc ctgcccttcc tcgacggcat cgagatctcg gtcaccttcg ccggccatac
     3421 cgtccacatc gtcggcctgg gcgtcgaccc cggcgaggag gcgctgcgcg cgggcgtcgc
     3481 ggcggtgcgc gccggccgcg agctgcgcgc ccgccagatg gccgacggcc tggccaaggc
     3541 cggcatccgg ggcgcgtacg aaggcgccgt gaagctggcg cccaacccgg acctcgtctc
     3601 tcgcacccac ttcgcgcgcc acctggtgaa cacgggcgtc gcgccggaca tgcacgccgt
     3661 cttccgccgc tacctcaccg agggcaagcc cggctacgtc gagcaccgct gggcgaagct
     3721 gggcgacgcg gtgcgctgga tccgcggggc cggcggcgtc gccgtgatcg cgcatccggg
     3781 ccgctaccgc ttcacgccga ccgaggaata cgcgctcttc accgagttcc aggcccatgg
     3841 cggcgaggcg gtcgaggtcg tgaccggcag ccaccacgcg ggcgacgtcg ccaagtacac
     3901 ggagatggcg ctcgagttcg gcctgctcgc ctcgcggggc agcgacttcc acgaccccgg
     3961 cgagagccga ctcgacctcg ggctgctgcc cgaccggacg gcgctgtcgc cgcgcctgac
     4021 gccggtgtgg tcgatctggc ccgagctcgc ggccgccgcg ggcgctcagg gcacgtcgtg
     4081 acgcgcggcc ccgcgcacgc gcatccgctg caaggcatcg ccctcgtcct cgtggccgcg
     4141 gcatgcttcg ccgggctgga ctcgagcacc cgcgcgctca cccaggcctc ggggcgctgg
     4201 cacatgccgg tgctggtcgt gctgacctca cgctacaccc tgcaggccgt cgtcatggcg
     4261 atctggctgg cgccgcgcgg gcgcgcgggc ttcgcggcgg cgcatccgcg cttccaggcg
     4321 ctgcgcggcg ggctgctgct ggccaccagc ctgtgctcgt tcttcggcgt gagcctgatg
     4381 cccgtgccgg agtacaccgc gatcaacatg atcacgccgc tgctggtcac cgtgctggcc
     4441 gcgaccgtgc tgcacgagcg cgtgtcgacg ctgcgctggg cgctggtggc cggcggcttc
     4501 gcgggcacgc tggtcgtcat ccggccgggc tcgggcgcat tcggctgggt ggtcgcgctg
     4561 cccgtgctgg gcgcgatcgc ctatgcgagc ttccaggtgc tcacgagccg cctggcgggt
     4621 gccgaggatc cgctgacaac gcacttctac accggcttcg tcggcgcggc ctgcctgctg
     4681 cccgcgctgg cggccgcgac ctcggtgcaa ggcctcgcct ggacggcgag gatggatgcg
     4741 tccggctggg ggctgctcgc gcttggcggg gcgctgggca cggcgggaca cctgctgctg
     4801 atcctcgcgc tcggccgcgc cggcaccgcg acgctgatgc ccttcgtcta cacccagatc
     4861 ggcttcgccg cgctgatcgg cgtggtcgcg ttcggccact ggccggacgg ctgggcgctc
     4921 gccggcatgg cggtcatcgc ggcctgcggc gcgagctcgg ccgcgctgaa cctgcgccgc
     4981 cccggtgcgg cggcgccggc cgccgcggtg gacatc
//
LOCUS       DASVLN010000502         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_240228, whole genome shotgun sequence.
ACCESSION   DASVLN010000502 DASVLN010000000
VERSION     DASVLN010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240228"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VF453_12335"
     CDS             complement(<1..367)
                     /locus_tag="VF453_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX7688491.1"
                     /translation="MDDTHPTAAARGDADRLPTFADLPGPAPRWWGFPLLAAMRRDYL
                     GFVAGLQRRHGDLVASRIVFERAVDVCSPDGVREVLVDHAGKLVRWERGVEVFEEALG
                     RGVLVTEGAEWQRQRRMLHG"
     gene            503..1402
                     /locus_tag="VF453_12340"
     CDS             503..1402
                     /locus_tag="VF453_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7688492.1"
                     /translation="MSAVAEPVAPISTAVNRVWLPRVSLASCIRGTMTRDTRHAGLAP
                     EQNFNYFPATPLCSISWWIEGRAERVLGPFPERPAALDDPRERIDARIVLGGPFNAPS
                     ASWNPGPMQGVMLMFMPDALQLMTGFDPAAHLNRLSDIRATLPADWCAMCEQVLSDPD
                     DAARVRRIEDFLEPRWAAVRPRHALAGHRYQDWAQGLAMRAAQSSAGSSLRQIERRIK
                     LWAGQPMRELQGVSRLERAFFDGVAAEEAGRLNFAELAAGTGYADQAHLTRVTRRLTG
                     FSPAELNRRIHAEEPFWAYRAWM"
     gene            complement(1413..>2284)
                     /locus_tag="VF453_12345"
     CDS             complement(1413..>2284)
                     /locus_tag="VF453_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7688493.1"
                     /translation="GGAATPLALAPPTPDEFGDVEAEELTATAADGTAIPLSIVHRKG
                     LKPDGSHLAYLTGYGAYGIALKPRPVSRWLPLLEDGGVYAVAHVRGGGEFGEDWHRAG
                     FQATKPNTYRDLIACAQALVARGWTRPERLAIEGASAGGITAGMALAERPDLFRVAFI
                     DVGDANPLRSEYGTDGAANAAEYGSAADEAGFRALLAVDATQHVRKGVLYPAVMLSAG
                     MNDPRVAPWQPGKMAAKLQAATASGRPVLFRVDFDAGHGIGSTRAQRDGLTADQLAFF
                     YWQAGVPGYQPAP"
BASE COUNT          283 a          856 c          859 g          286 t
ORIGIN      
        1 ccccgtgcag catgcggcgc tggcgctgcc actcggcgcc ctcggtcacg aggacgccgc
       61 gacccagcgc ctcctcgaag acctcgacgc cgcgctccca gcgcacgagc ttgccggcgt
      121 ggtcgaccag cacctcgcgc acgccgtcgg gcgagcagac gtcgaccgcg cgttcgaaga
      181 cgatgcgcga cgcgacgagg tcgccgtggc ggcgctgcag gccggcgacg aagccgaggt
      241 agtcgcgccg catcgccgcg agcagcggga agccccacca gcgcggggcg ggcccgggga
      301 ggtcggcgaa ggtgggcagg cggtcggcgt cgccgcgcgc ggcggccgtg gggtgggtgt
      361 cgtccatgcg gcgcatgctg cgcgtccggg acggcgccgg tattgaacga agtcgacacc
      421 gcggggccat gcgccgcgcg gcgcgggcgc ttgcgtacac tcgcctcgac gcgagcgccg
      481 atgcccgccg cgtcgccttc cgatgagcgc cgtcgccgaa cccgtcgccc cgatctccac
      541 cgccgtcaac cgcgtgtggc ttccgcgcgt gtcgctggcc agctgcatcc gcggcacgat
      601 gacgcgcgac acgcgccacg ccgggctggc gccggagcag aacttcaact acttcccggc
      661 cacgccgctg tgctcgatca gctggtggat cgagggccgc gccgagcgcg tgctgggccc
      721 gttcccggag cggccggcgg cgctcgacga cccgcgcgaa cggatcgacg cgcgcatcgt
      781 gctcggtggc ccgttcaacg cgccttcggc gagctggaac ccggggccga tgcagggcgt
      841 gatgctgatg ttcatgcccg acgcgctgca gctgatgacg ggcttcgatc ccgcggcgca
      901 cctgaaccgc ctgagcgaca tccgagcgac gctgccggcc gactggtgcg ccatgtgcga
      961 gcaggtgctg tccgatcccg acgacgccgc gcgcgtgcgc cgcatcgagg acttcctcga
     1021 gccgcgctgg gccgccgtgc ggccgcgcca cgcgctggcc ggccatcgct accaggactg
     1081 ggcgcagggc ctcgcgatgc gcgcggcgca gtcgtcggcg ggctcgagcc tgcgccagat
     1141 cgagcgccgc atcaagctct gggccggcca gccgatgcgc gagctgcagg gcgtgagccg
     1201 gctcgaacgc gccttcttcg acggcgtcgc cgccgaggag gccgggcggc tgaacttcgc
     1261 cgagctcgcc gccggcaccg gctacgccga ccaggcccac ctcacgcgcg tgacgcgccg
     1321 gctgaccggc ttctcgccgg ccgagctcaa ccgccgcatc cacgccgagg agccgttctg
     1381 ggcctatcgc gcgtggatgt agcgcgcccg gctcaaggcg ccggctggta gcccggcacg
     1441 ccggcctgcc agtagaagaa cgcgagctgg tcggcggtca gcccgtcgcg ctgcgcgcgc
     1501 gtcgagccga tgccgtggcc ggcgtcgaag tccacgcgga acaggacggg ccggccgctg
     1561 gccgtcgccg cctgcagctt cgcggccatc ttgcccggct gccagggcgc gacgcgcggg
     1621 tcgttcatgc ccgcgctgag catcacggcc ggatagagaa cgcccttgcg cacgtgctgg
     1681 gtggcgtcga cggccagcag cgcgcgaaag ccggcctcgt cggcggcgct gccgtactcc
     1741 gcggcgttgg cggcgccgtc ggtgccgtac tccgagcgca gcgggttggc gtcgccgacg
     1801 tcgatgaagg cgacgcggaa caggtcgggc cgctccgcca gcgccatgcc cgccgtgatg
     1861 ccgccggcgc tcgcgccctc gatcgccagg cgctcgggcc gcgtccagcc gcgcgcgacg
     1921 agcgcctggg cgcacgcgat caggtcgcgg taggtgttgg gcttggtcgc ctggaagccg
     1981 gcgcgatgcc agtcctcgcc gaactcgccg ccgccgcgca cgtgcgcgac cgcgtagacg
     2041 ccgccgtcct ccagcagcgg cagccagcgc gagaccgggc gcggcttgag cgcgatgccg
     2101 taggcgccat accccgtgag gtacgcgaga tgcgagccgt ccggcttcag gcccttgcgg
     2161 tggacgatgg acagcgggat cgccgtgccg tcggcggcgg tggccgtgag ttcctcggcc
     2221 tcgacgtcgc cgaactcgtc gggcgtcggc ggcgccagcg cgagcggcgt cgccgcgccg
     2281 ccgc
//
LOCUS       DASVLN010000503        11092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_510982, whole genome shotgun sequence.
ACCESSION   DASVLN010000503 DASVLN010000000
VERSION     DASVLN010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11092
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510982"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VF453_12350"
     CDS             <1..705
                     /locus_tag="VF453_12350"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEX7688494.1"
                     /translation="RRLAIYRANLAATADKALAGAYPVVRQAVGEEFFRGLAREYVRA
                     APSTGGDLADFGDRFADFLAGFEHVRDLPWLPDLARVEWAAHRACGAADGTPWDAGAL
                     ATVAPEDQAAIRFAWAPGTAIVESPWPVVRVWTIHQPGFDGAFEVDWQVPERALVARE
                     GLAVTVTALAAGEAVFVARSLAGAPLGEAAARALEADAAFDLGALLARAVGAGLLRGF
                     TLCQEPQDPISDENPR"
     gene            689..1159
                     /locus_tag="VF453_12355"
     CDS             689..1159
                     /locus_tag="VF453_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7688495.1"
                     /translation="MKTLVSRALAATALLDRLQPLALLALRLYVSSVFVRSGLVKVSD
                     WGATLALFQDEYHVPLLPPGVAACVGAFGELFFPVLVTLGLAGRFGAAGLFAVNAMAV
                     ISYPQLWGFDCPAAIQSHFIWGTILLALVVFGPGRWSLDELVLRRLGLARPARA"
     gene            complement(1176..1535)
                     /locus_tag="VF453_12360"
     CDS             complement(1176..1535)
                     /locus_tag="VF453_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7688496.1"
                     /translation="MKTILVVDDELGSAEVLSMILAEEGYRTFCAVNGALALAQARDV
                     VPDLLIVDYMMPLMNGAELSRELRADPAFAHTKIILNSGLPEYAIREQFDGYDAFLRK
                     PFKIEMLLQLVHELIGR"
     gene            complement(1561..3060)
                     /locus_tag="VF453_12365"
     CDS             complement(1561..3060)
                     /locus_tag="VF453_12365"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX7688497.1"
                     /translation="MTTIVDNVPPVERLRSAVPGLDTILGGGFFRSGVYIIQGLPGCG
                     KTILANQVCYGHIAQGGAAVYVTLLAESHSRMIQHLSTLSFFDVKAFPDKLAYISAFH
                     DLESNGLKGLMTVLRREMRTRRVGVLVLDGLVAASEAAPTDLDLKKFIHELQSIAVLQ
                     DCTVLLLTSGNIQRMAAEHTMVDGLIELEDKLFDARSERSIQVRKFRGAGPLRGKHAL
                     RIDNDGISVFPRIESLYRHTTLDATPGGPVASGIASLDRLLAIGGIPRSSSTVVVGST
                     GTGKTTMGIQFLAASTPEEPGLHLGFFEGPDRLRGKAASIGRPLEALEASGAIELMWQ
                     SPGESMLDEIADRLLTAVSRRGVRRLVIDGLSGFLESAVYPERVSRFFSCLANELRNR
                     QVTVMMTLETRDVVSSVVSMPWGISGLVDNLFFLRFVHDEGHVERLLTIIKMRDTDYH
                     AGLRRLHIDGRGMHLAGVFAADGDVIPSAQPVSPPVAPPESPVGGDAKA"
     gene            complement(3057..3860)
                     /locus_tag="VF453_12370"
     CDS             complement(3057..3860)
                     /locus_tag="VF453_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7688498.1"
                     /translation="MTDVSPSAQRLTDPAGAAVADECAHLREEIAVRDDILSLAAHEL
                     RNPLHALALQLALARTMAQSGEREQAVERIHRAELTLRRYSERVTVLMELLASPGAVY
                     PLAPRPVDVQALLATLVDSLEQEARSRAIAVTLDAADAGIRSVDPVALEQVVDNLLLN
                     AFKHSRATQVDVRVRAAAGGWTVEVVDNGGGIAPADQALIFEKFAVARHSTRGSGSGL
                     GLWIASRLVHAMRGAITLDSAPGAGCAFAVRIPDHPSDLRERDDMSTAQ"
     gene            complement(4055..5824)
                     /locus_tag="VF453_12375"
     CDS             complement(4055..5824)
                     /locus_tag="VF453_12375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX7688499.1"
                     /translation="MTSTIPAAVRRSLRETFGFTELRDGQRDVIARVLRGEDTLAIMP
                     TGAGKSLCYQLPALHLPGTTIVVSPLISLMKDQAEKLGEAGVAAEQLNSALKASEQDA
                     SMSRIAEDDSAIVFATPERLADPEFRAAMAGRHVSLLVVDEAHCISQWGHDFRPAYLE
                     IGAALEALGHPTVLALTATATSEVVDDIRHQLHRPGLHVISTGMYRPNLRLRVLQVTN
                     EVEKFERLLEAIRAQEGGGIVYTATVKACSELHERLVAAGESATIYHGKLPTAERNAN
                     QDAFMTGERRVMVATNAFGMGIDKPDIRFVVHYQMPGSAQAYYQEAGRAGRDGEPADC
                     VLIFDRRDRQVQQFFLARKYPGADDLQRLVDALALAPDGATLAEVVSRTDGLPKTRAS
                     VGLSLLREAGLACCDAAHGWRRCGPKLVQRRELGALAADYARKSERDHEALERMVFYA
                     QTGFCRWRVLLDAFDEPVPFDDGRCGECDNCRRAPPAEACIEVARPASPPIEAPALAA
                     ARGLRRDDAVSVPRYGIGRVVSTRGEEITVAFPDRSSRTFLASFLTRVDDPETGELNA
                     AVLSAAPVRGRTRARARPQAAPS"
     gene            5917..6207
                     /locus_tag="VF453_12380"
     CDS             5917..6207
                     /locus_tag="VF453_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7688500.1"
                     /translation="MFVVTLTYIKPVEEIDALMDAHVAWLKSHYASGLFVASGRRVPR
                     TGGVILARSGDEAGLRAALASDPFALHQAARFDVVEFTATMTAPGAEVLKSL"
     gene            complement(6230..6805)
                     /locus_tag="VF453_12385"
     CDS             complement(6230..6805)
                     /locus_tag="VF453_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX7688501.1"
                     /translation="MTRIAIVFYSGYGHTAKQAEAVHAGAASVEGAQVSSHRIDANGE
                     LSDAAWADISAADAIIYGSPTYMGGPAWQFKKFADASSKPWFTQAWKDKVAAGFTNSA
                     SVNGDKFSTIEYLWTLSQQHAQVWVGTGLMPANTKAHGPSDVNWTAGFAGALAISPSD
                     ASPDEAPREGDLETARLHGKRVAEFAKRLRG"
     gene            complement(6926..7813)
                     /locus_tag="VF453_12390"
     CDS             complement(6926..7813)
                     /locus_tag="VF453_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7688502.1"
                     /translation="MISMQGVPSSLIARHESWVRKQASALSRRLPANVEKADLIQAGL
                     IGVAQAAMNFEWEGDPDSDEGREAFLRYAQMRVKGAMLDELRQMDQLSRSQRRKYKVV
                     QIARERWMQGHDQPPTLTELAEVCGIEVEEIAELEQAASTAQTQSLSEDDDDTEYHAP
                     AGQAATPTDEVEARVDTAILLRRLEKFFATLPERERQVIDAYMGIGMSTEEVAESLGV
                     STSRVSQIYQATVKRISQHFSHQEQRAVDRYPARSGPEFDKLVASREASQGWGGLLEQ
                     ALTAPAERYGKRRKAAGSR"
     gene            8050..9465
                     /locus_tag="VF453_12395"
     CDS             8050..9465
                     /locus_tag="VF453_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7688503.1"
                     /translation="MDSDRTRRTLPWIVATALFMEQLDSTIVNTGIPAMAASLGVTPL
                     SLKAVATSYILSLAVSIPVSGWLAERFGTRRVFLFALGMFTVSSVLCGAAPNASLLVA
                     SRVPQGIAAAMMMPVGRSAVVRAFPKSELLRVMNFIIIPALIGPLLGPTIGGLIVHWA
                     SWREIFFVNVPVGAVAFWLGRRHMPDFRNPAPRPLDVIGLVLFGTGAALLSWLLEIFG
                     EHELPALQTAVLLLLSLALLLAYGWHATREPHPLLPLGIFRVRTFRISVLGGFVTRLG
                     IGGMPLLLPLLYQLGLGLPAWESGLLMMPAAAGAMFMKVLSSRLLRRFGYRQVLVFNT
                     MLMATAIGGYALVGTRTPVAAILLVGLGMGLVNSLQFSSLNTMAYADIEPADASIAST
                     IASTLQQMSLSFGLAMGSLITAWYLANLPQTDHVAVTRALHHGFETLAVVTLASSLAF
                     RLLRPDDGESVSKGAPAGDEE"
     gene            complement(9689..10006)
                     /locus_tag="VF453_12400"
     CDS             complement(9689..10006)
                     /locus_tag="VF453_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase with chaperone activity"
                     /protein_id="HEX7688504.1"
                     /translation="MSEDNQIVVPPSFVALFVPPGRIRPTETREAITARYEFCEALAT
                     MLVERAQTLQWQLGITEADVLARIREGLTGPQAPVPAEEGEWVLSRLAELLEWERPAW
                     KKD"
     gene            10105..>11092
                     /locus_tag="VF453_12405"
     CDS             10105..>11092
                     /locus_tag="VF453_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HEX7688505.1"
                     /translation="MRELIPLLIAHGALVIFLITLVARVGAPVPAAPLLVVAGGVAMA
                     GQLSLLVCLVAAVLANVLGDAVWYQAGRWRGHRVMRLLCKISLSPDTCVRQSEGILSR
                     WGGSSLIAAKFVPGVSVVAAPMAGALGMSWTRFVAYDLGAGLIWSAAFMMLGVVFAGQ
                     IEVVLDLMTEFGSLAAVVIAIALACMLSWRYLRRRWMLSERYAPRIAVAELREMMRRG
                     EPPVVIDVRSSVATEHDPRRLPGAIAATLADLPAAIARLPRDRQIVVYCNCPNEVSAL
                     RGVRILADHGLHRSRALVGGLDAWLAIEAASPLEAAGAEPAVVAIVEPASPAG"
BASE COUNT         1411 a         3997 c         3945 g         1739 t
ORIGIN      
        1 cggcggctcg cgatctaccg cgccaacctc gcggcgacgg ccgacaaggc gctcgccggt
       61 gcctaccccg tcgtccgcca ggcggtcggc gaggaattct tccgcggcct cgcccgcgag
      121 tacgtcaggg cggccccgtc caccggcggc gacctcgccg acttcggcga tcgcttcgcc
      181 gacttcctcg cgggcttcga gcacgtgcgc gacctgcctt ggctgcccga cctagcgcgc
      241 gtcgaatggg ccgcgcaccg ggcctgcggc gccgccgacg gcacgccctg ggacgcaggc
      301 gcgctcgcga cggtcgcccc cgaggaccag gccgcgatcc gcttcgcctg ggcgccgggc
      361 accgcgatcg tcgagtcgcc ctggcccgtc gtgcgggtgt ggacgatcca ccagcccggc
      421 ttcgacggcg cgttcgaggt cgactggcag gtgcccgagc gcgccctggt cgcccgcgag
      481 ggcctcgccg tcacggtcac cgcgcttgca gccggcgagg cggtcttcgt cgcgcgctcg
      541 cttgccgggg cgccgctggg cgaggccgct gcccgcgcac tcgaggccga tgccgccttc
      601 gacctcgggg ccctgctcgc gcgcgcggtc ggcgcggggc tgctccgcgg attcaccctc
      661 tgccaggaac cacaggatcc catttccgat gaaaaccctc gttagccggg cgctcgccgc
      721 cacggcgctc cttgaccgcc tgcagccgct cgcgctgctg gccctgcggc tgtatgtctc
      781 gtccgtgttc gtgcgatcgg ggctcgtcaa ggtctccgac tggggcgcga cgctcgcgct
      841 gttccaggac gagtaccacg tcccgctgct gccgcccggc gtcgcggcct gcgtcggcgc
      901 gttcggcgag ctgttcttcc ccgtgctggt cacgctgggc ctggccggcc gcttcggcgc
      961 cgcgggcctg ttcgcggtca acgcgatggc ggtgatctcc tacccgcagc tgtggggctt
     1021 cgactgcccg gccgcgatcc agtcgcactt catctggggg acgatcctgc tcgcgctcgt
     1081 cgtcttcggc ccgggccggt ggtcgctcga cgagctggtg ctgcgccggc tcgggctcgc
     1141 gcgtcccgcg cgcgcgtgag gcgacggcga ccggttcagc gcccgatgag ctcgtgcacg
     1201 agctgcagca gcatctcgat cttgaacggc ttgcgcagga acgcgtcgta gccgtcgaac
     1261 tgctcgcgga tcgcgtactc gggcagcccg ctgttgagga tgatcttggt gtgcgcgaac
     1321 gccgggtcgg cgcgcagctc gcgcgagagc tccgcgccgt tcatcagcgg catcatgtag
     1381 tcgacgatca gcaggtcggg caccacgtcg cgtgcctgcg cgagcgcgag cgcgccgttg
     1441 accgcgcaga acgtgcgata gccttcctcc gccaggatca tgctcaggac ctccgcgctc
     1501 ccgagttcgt cgtcgacgac aagaatggtc ttcacgcggg cgttccttcg cctcgtaccg
     1561 tcaggccttc gcgtcgccgc cgacggggct ctcgggcgga gccaccggcg gactgacggg
     1621 ctgcgccgac gggatcacgt cgccgtcggc cgcgaacacg ccggccaggt gcatgccgcg
     1681 cccgtcgatg tgcagccgac gcaggccggc gtgatagtcc gtgtcgcgca tcttgatgat
     1741 ggtcagcagt cgttcgacgt ggccctcgtc gtgcacgaag cgcaggaaga acaggttgtc
     1801 gaccagcccc gagatgcccc acggcatcga caccacgctg gagacgacgt cgcgcgtctc
     1861 cagcgtcatc atcacggtga cctggcggtt gcgcagctcg ttggccaggc acgagaagaa
     1921 gcggctgacg cgctcgggat acacggccga ctcgaggaag cccgacaggc cgtcgatcac
     1981 cagccggcgc acgccccggc gcgagacggc cgtcagcagg cggtcggcga tctcgtcgag
     2041 catggactcg cccggcgact gccacatcag ctcgatcgcc cccgacgcct ccagcgcctc
     2101 gagcggccgc ccgatcgacg ccgccttgcc gcgcagccgg tccggtcctt cgaagaagcc
     2161 gaggtgcagg cccggctcct cgggcgtgct ggccgccagg aactggatgc ccatcgtcgt
     2221 cttgcccgtg cccgtcgagc cgacgacgac ggtggaactg ctgcggggga tgccgccgat
     2281 cgccagcagc cggtccagcg aggcgatgcc gctcgcgacc gggccgcccg gcgtggcatc
     2341 gagcgtcgtg tgccggtaca gagactcgat gcgcgggaac acgctgatgc cgtcgttgtc
     2401 gatgcgcagc gcgtgcttgc cccgcagcgg gccggcgccg cggaacttgc gcacctggat
     2461 cgagcgctcg gatcgggcat cgaacagctt gtcctccagc tcgatcaggc cgtcgaccat
     2521 cgtgtgctcg gcggccatgc gctggatgtt gccgctggtc agcagcagca cggtgcagtc
     2581 ctgcagcacg gcgatgctct ggagctcgtg gatgaacttc ttcaggtcga ggtcggtggg
     2641 cgcggcctcg gacgccgcga ccaggccgtc cagcaccagc acgccgaccc ggcgggtgcg
     2701 catctcgcgc cgcagcacgg tcatcaggcc cttcaggccg ttgctctcga ggtcgtggaa
     2761 ggcgctgatg taggccagct tgtcgggaaa cgccttgacg tcgaagaacg acagcgtcga
     2821 caggtgctgg atcatgcgcg agtgcgactc ggccagcagc gtcacgtaga ccgccgcgcc
     2881 gccctgggcg atgtggccgt agcagacctg gttggccagg atcgtcttgc cgcagcccgg
     2941 cagcccctgg atgatgtaga cgccgctgcg gaagaagccg ccaccgagga tggtgtcgag
     3001 gccgggcacc gcgctgcgca ggcgctcgac cggcgggacg ttgtcgacga tggtggtcat
     3061 tgggcagtgc tcatgtcgtc cctctcgcga aggtcgctgg gatggtcggg gatgcggacc
     3121 gcgaaggcgc agcccgcgcc gggcgcgctg tccagcgtga tcgcgccccg catggcatgc
     3181 accagccgcg acgcgatcca cagcccgagc ccgctgcccg acccgcgggt cgagtgccgg
     3241 gcgacggcga acttctcgaa gatcagggcc tggtcggccg gcgcgatgcc gccgccattg
     3301 tccaccacct cgaccgtcca gccgcccgcc gccgcgcgca cccgcacgtc cacctgcgtg
     3361 gcgcgcgagt gcttgaaggc attgagcagc aggttgtcga ccacctgttc cagcgccacc
     3421 ggatcgacgc tgcggatgcc ggcgtccgcc gcgtccagcg tcaccgcgat ggcgcgcgag
     3481 cgcgcctcct gctccaggct gtcgaccagc gtcgccagca gcgcctgcac gtccaccggc
     3541 cgcggcgcga gcggatagac cgcgcccggc gaggccagca gctccatcag cacggtgacg
     3601 cgctcggaat agcggcgcag cgtgagctcg gcgcggtgga tgcgctcgac ggcctgctcg
     3661 cgctcgcccg actgcgccat cgtgcgcgcc agcgccagct gcagcgcgag cgcatgcagc
     3721 ggattgcgca gctcgtgcgc cgccagcgag aggatgtcgt cgcgcacggc gatctcctcg
     3781 cgcagatgcg cgcactcgtc cgcgacggcg gctccggcgg gatcggtcag gcgctgcgcg
     3841 gacggggaga cgtcggtcaa ggcggaggct tcatgggccg gaagcggcgc gatatacggc
     3901 accgggtatg ccgctggcgc acgggcgcag tcgcggcccg gcaactttac cgtgcaaacg
     3961 accgcctcgc ttgccgatga acgcgatgag tgcgtgcgcg gccgcgacga ccgcgcacca
     4021 gcaatcggca ttcccgcggc gcgcggacga cgcttcagct cggcgccgcc tgcggcctcg
     4081 cgcgcgcccg cgtgcgtcct cgcaccggtg ccgcggagag caccgcggcg ttgagctcac
     4141 ccgtctcggg atcgtccacg cgcgtcagga acgacgcgag gaaggtacgc gaactgcggt
     4201 ccgggaaggc gacggtgatc tcctcgccgc gcgtggagac gacccgcccg atgccgtagc
     4261 gcggcaccga gacggcgtcg tcgcgcctca gcccccgtgc cgccgccagc gccggcgcct
     4321 cgatcggcgg cgacgccggg cgcgcgacct cgatgcacgc ctcggcgggc ggcgcgcggc
     4381 ggcagttgtc gcattcgccg cagcggccgt cgtcgaaggg cacgggctcg tcgaacgcgt
     4441 ccagcaggac gcgccaacgg cagaagccgg tctgcgcgta gaacaccatg cgctcgagcg
     4501 cctcgtggtc gcgctcgctc tttcgcgcgt agtccgcggc cagtgcaccc agctcgcgcc
     4561 gctgcacgag cttcgggccg cagcgccgcc agccgtgcgc cgcgtcgcag caggccaggc
     4621 cggcctcgcg cagcaggctc aggccgaccg aggcgcgcgt cttgggcagg ccgtcggtgc
     4681 gcgagaccac ctcggcgagc gtcgccccgt cgggcgcgag ggcgagcgcg tcgacgaggc
     4741 gctgcaggtc gtcggcgccc ggatacttgc gcgcgaggaa gaactgctgc acctggcggt
     4801 cgcggcggtc gaagatcaac acgcagtcgg ccggctcgcc gtcacggccg gcacgcccgg
     4861 cctcctggta gtaggcctgc gcgctgcccg gcatctggta atggacgacg aagcggatgt
     4921 cgggcttgtc gatgcccatg ccgaacgcgt tggtcgcgac catcacgcgg cgctctcccg
     4981 tcatgaacgc gtcctggttg gcgttgcgct cggcggtcgg cagcttgccg tggtagatcg
     5041 tcgccgactc gccggccgcg accaggcgct cgtgcagctc ggagcaggcc ttcacggtgg
     5101 cggtgtagac gatgccgccg ccctcctggg cgcggatcgc ctccagcagg cgctcgaact
     5161 tctcgacctc gttggtgacc tgcagcaccc gcagacgcag gttgggccgg tacatgccgg
     5221 tcgagatcac gtgcagcccc gggcgatgga gctggtggcg gatgtcgtcg acgacctcgg
     5281 aggtcgcggt ggccgtcagc gccagcacgg tggggtggcc cagcgcctcg agcgccgcgc
     5341 cgatctccag gtaggccggc cggaagtcgt ggccccattg cgagatgcag tgcgcctcgt
     5401 cgaccaccag cagcgagacg tggcggcctg ccatcgcggc gcggaactcg gggtcggcca
     5461 ggcgctcggg cgtggcgaag acgatcgcgc tgtcgtcctc ggcgatccgc gacatcgacg
     5521 catcctgctc gctcgccttg agcgcgctgt tgagctgctc ggccgcgacg ccggcctcgc
     5581 cgagcttctc ggcctggtcc ttcatcagcg agatcagcgg ggagacgacg atggtcgtgc
     5641 ccggcaggtg cagcgcgggc aactggtagc acagcgactt gcccgcgccg gtgggcatga
     5701 tcgcgagcgt gtcctcgccg cgcaggacgc gcgcgatcac gtcgcgctgg ccgtcgcgca
     5761 gctcggtgaa gccgaaggtc tcgcgcagcg aacgccgcac cgcggccgga atcgtggagg
     5821 tcatgcgcag cgagggggca agcgatgtgc ctcgcggtcc tccgctacag tggcgcgtcc
     5881 gtttccgttt ccgcgccgcg gcgctcgccc tcacgcatgt tcgtcgtcac gctcacctac
     5941 atcaagcccg tcgaggagat cgacgccctc atggacgccc acgtcgcctg gctcaagtcg
     6001 cactacgcgt cgggcctgtt cgtcgcctcc ggacggcgcg tgccgcgcac gggcggcgtg
     6061 atcctcgccc gctccggcga cgaggcgggg ctgcgcgcgg cgctcgcgtc cgatccgttc
     6121 gccctgcacc aggccgcgcg cttcgacgtc gtcgagttca ccgcgacgat gacggcgccc
     6181 ggcgccgagg tgctgaagtc gctttgacgt cgcggaccgg cgcccgccct cagccgcgca
     6241 ggcgcttcgc gaactcggcg acgcgctttc cgtgcaggcg cgcggtctcg agatcgccct
     6301 cgcgcggcgc ctcgtccggc gaggcatcgg acggcgagat cgccagcgcg cccgcgaacc
     6361 cggcggtcca gttgacgtcg gacgggccgt gcgccttcgt gttggcaggc atgaggcccg
     6421 tgcccaccca gacctgcgca tgctgctgcg acagcgtcca caggtattcg atggtcgaga
     6481 acttgtcgcc gttgacggag gccgagttcg tgaacccggc agcgaccttg tccttccagg
     6541 cctgcgtgaa ccacggcttg gacgacgcgt cggcgaactt cttgaactgc cacgccggcc
     6601 cgcccatgta ggtggggctg ccgtagatga tcgcgtcggc ggcactgatg tcggcccagg
     6661 cggcgtcgct cagctcgccg ttggcgtcga tgcggtgcga cgacacctgg gcgccctcga
     6721 cggaggcggc accggcgtgg acggcctcgg cctgcttggc ggtgtggccg tagccgctgt
     6781 agaagacgat cgcgatccgg gtcatgcgtg gttcctcgat ggatggtggc ggcttccgga
     6841 cgggaagcga gcgccgattg tgtccagggt cgagtctccc cacgcacgcg gggacaatgc
     6901 gtgcgcccgc cgcggtcggt gccggtcagc ggctgcccgc ggccttgcgg cgcttgccgt
     6961 agcgttccgc gggcgcggtg agcgcctgtt ccagcagccc gccccagcct tgggacgcct
     7021 cgcgcgaggc gaccagcttg tcgaactcgg ggccggagcg cgccggatag cggtccaccg
     7081 cgcgctgttc ctggtgcgag aagtgctgcg agatgcgctt gacggtcgcc tggtagatct
     7141 gcgagacgcg cgaggtgctc acgcccagcg actccgcgac ttcctcggtg ctcatgccga
     7201 tgcccatgta ggcgtcgatg acctggcgtt cgcgctcggg cagcgtggcg aagaacttct
     7261 cgagccggcg cagcaggatc gcggtgtcga cgcgcgcctc gacctcgtcc gtcggcgtcg
     7321 ccgcctgccc ggcgggcgcg tggtattcgg tgtcgtcgtc gtcctcggac aggctctgcg
     7381 tctgggcggt cgaggcggcc tgctcgagct cggcgatctc ctcgacctcg atgccgcaga
     7441 cctcggccag ctcggtcagc gtgggcggct ggtcgtgtcc ctgcatccag cgctcgcgcg
     7501 cgatctgcac caccttgtac ttgcggcgct gggagcggct cagctggtcc atctggcgca
     7561 gctcgtcgag catcgcgccc ttgacgcgca tctgggcgta gcgcaggaac gcctcgcgac
     7621 cctcgtcgct gtcggggtcg ccttcccatt cgaagttcat cgccgcctgc gcgacgccga
     7681 tcaggcccgc ctggatcagg tcggccttct cgacgttggc cggcagccgg cgcgacagcg
     7741 cgctcgcctg cttgcgcacc cacgactcgt ggcgcgcgat caaggacgac gggacaccct
     7801 gcatcgaaat catgacgcca tgatatgcaa gctatgggcg gacccgaccg atcttttttc
     7861 tcgaaatggg cggccggcgt gaagcaatgg agccgaaccc gccttttttc ctcccggcga
     7921 gccacgatgc gaaccgccgc aggccgatac aagaggccgc cttgcgcgac cgtggcgcgg
     7981 ctcagggcca gtcggtcgcg tccacccgcg gacgcgcgcc gggcggccgg acgcgccaga
     8041 atgcctgcga tggattccga ccgcacccgg cgcaccctgc cctggatcgt cgcgaccgcg
     8101 ctgttcatgg agcagctcga ctcgacgatc gtcaacaccg gcattccggc gatggcggcc
     8161 agcctggggg tcacgccgct gagcctgaag gccgtggcca cgagctacat cctgtcgctg
     8221 gccgtctcga tcccggtcag cggctggctc gcggagcgct tcggcacgcg gcgcgtcttc
     8281 ctgttcgcgc tggggatgtt cacggtctcc tcggtgctgt gcggcgcggc gcccaacgcg
     8341 agcctgctgg tcgcctcgcg cgtgccccag ggcatcgcgg cggcgatgat gatgccggtc
     8401 ggccgcagcg cggtcgtgcg cgcgttcccg aagtcggagc tgctgcgggt gatgaacttc
     8461 atcatcatcc cggcgctgat cgggccgctg ctgggcccga cgatcggcgg cctgatcgtg
     8521 cactgggcgt cgtggcgcga gatcttcttc gtcaacgtgc cggtcggcgc ggtcgcattc
     8581 tggctcggcc gacgccacat gcccgacttc cgcaacccgg cgccgcgtcc gctggacgtg
     8641 atcggcctcg tgctgttcgg caccggcgcc gcgctgctgt cgtggctgct ggagatcttc
     8701 ggcgagcacg agctgccggc gctgcagacg gccgtgctgc tcctgctgtc gctcgcgctc
     8761 ctgctggcct acggctggca cgcgacgcgc gagccgcatc cactgctgcc gctgggcatc
     8821 ttccgcgtgc gcacgtttcg catctcggtg ctgggaggct tcgtcacgcg gctgggcatc
     8881 ggcggcatgc cgctgctgct gccgctgctc taccagctcg gcctggggct accggcgtgg
     8941 gagtcgggcc tgctgatgat gcccgcggcc gcgggcgcga tgttcatgaa ggtgctgtcc
     9001 tcgcgactgc tgcggcgctt cggctaccgg caggtgctgg tcttcaacac gatgctgatg
     9061 gccaccgcga tcggcggcta cgcgctcgtc ggaacgcgca cgcccgtggc ggcgatcctg
     9121 ctggtcgggc tcggcatggg gctggtcaac tcgctgcagt tctcgagcct gaacaccatg
     9181 gcctacgccg acatcgagcc cgccgacgcc tcgatcgcca gcacgatcgc gagcacgctg
     9241 cagcagatgt cgctgagctt cggcctggcg atgggttcgc tgatcacggc ttggtatctc
     9301 gcgaacctgc cgcagaccga ccacgtggcc gtcacgcgcg cgctgcacca cggcttcgag
     9361 acgctggcgg tcgtgacgct cgcgtcgtcg ctcgccttcc gcctgttgcg gccggacgat
     9421 ggcgagagcg tcagcaaggg cgcgccggcc ggcgacgagg aatgaactgg gccgcgatcg
     9481 acttgctttt cgtcaaattg ctgtcgcact cggcgcgtag actgcgaagc atccgaggtg
     9541 cgaaacaacc ggccgcaaat tgggcggatc gcagggaacc cgtcacccga gcactccccg
     9601 gatgccgtcg tcgccgccag gcacgcccgg caagatagcc cgccaccggc acaggtcgcg
     9661 ggcttttctt atgcccgcgc gacctgggtc agtccttctt ccaggcgggg cgctcccatt
     9721 cgagcagctc ggccagtcgc gagagaaccc actcgccctc ctcggccggc accggcgcct
     9781 gcggcccggt cagcccctcg cggatgcgcg cgagcacgtc cgcctcggtg atgcccagct
     9841 gccactgcaa tgtctgggcg cgctcgacca gcatcgtcgc gagcgcctcg cagaactcgt
     9901 agcgcgccgt gatcgcctcg cgcgtctcgg tgggccggat ccggccgggc ggcacgaaca
     9961 gcgcgacgaa ggacggcggc acgacgatct ggttgtcttc ggacatcgcc cgattgtcgc
    10021 cgcgccgacg cccctgcggc cggcaggccg agggcgcccg cggtatcctg tcgcctcgtc
    10081 ccggccgtca ccctccccga ttccatgcgc gagctcatcc cgttgctcat cgcccacggc
    10141 gcgctcgtga tcttcctgat cacgctggtc gcgcgcgtcg gtgcgccggt gccggcggcg
    10201 ccgctgctgg tcgtggccgg cggggtcgcg atggcgggtc agctgtcgct gctggtctgc
    10261 ctggtcgccg ccgtgctggc caacgtgctg ggcgacgccg tctggtacca ggccggccgc
    10321 tggcgcgggc accgggtgat gcggctgctg tgcaagatct cgctgtcgcc cgacacctgc
    10381 gtgcgccaga gcgagggcat cctctcgcgc tggggaggca gctcgctgat cgccgccaag
    10441 ttcgtgccgg gcgtctcggt ggtggcggcg ccgatggccg gcgcgctggg catgtcgtgg
    10501 acgcgcttcg tcgcctacga cctcggggcc gggctgatct ggtcggccgc cttcatgatg
    10561 ctgggcgtgg tcttcgccgg ccagatcgag gtcgtgctcg acctgatgac cgagttcggc
    10621 agcctcgccg ccgtcgtcat cgcgatcgcg ctggcctgca tgctgtcctg gcgctacctg
    10681 cgccggcgct ggatgctgag cgagcgctac gcgccgcgca tcgccgtggc cgagctgcgc
    10741 gagatgatgc gccgcggcga gccgccggtc gtgatcgacg tgcgctcgtc ggtcgccacg
    10801 gagcacgacc cgcgccgcct gccgggcgcc atcgccgcga cgctcgcgga cttgccggcc
    10861 gcgatcgccc gcctgccgcg cgaccgccag atcgtcgtct actgcaattg cccgaacgag
    10921 gtctcggccc tgcgcggggt gcgcatcctc gccgaccacg gactgcaccg ctcccgcgcg
    10981 ctcgtgggcg gcctggacgc ctggctggcg atcgaggcgg cctcgccgct ggaagccgcc
    11041 ggggccgagc cggcggtcgt ggcgatcgtc gagcccgcct cgcccgccgg cg
//
LOCUS       DASVLN010000504         2035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_187152, whole genome shotgun sequence.
ACCESSION   DASVLN010000504 DASVLN010000000
VERSION     DASVLN010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187152"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF453_12410"
     CDS             <1..204
                     /locus_tag="VF453_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase"
                     /protein_id="HEX7688506.1"
                     /translation="DGCLAFAREHRVVVEPACGASIAATLAGGAAFADARTVLVVACG
                     GVSSTYEQLQAWRDAFARDGLAG"
     gene            complement(402..1139)
                     /locus_tag="VF453_12415"
     CDS             complement(402..1139)
                     /locus_tag="VF453_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7688507.1"
                     /translation="MLVLVFVAGLWAGLQNTLAGGGSFVTLPALILAGLTPLAANITS
                     TVALFPGQVTAGVAGRRLVEGVRGLSFRVLLAISLAGGAVGGLLLLNTPATIFARLVP
                     WLVLFATAVFAWGSFRRRPVEGAAHLGPVGAGIAQFLIAIYGGYFGGGIGFLMLAALT
                     MAGLATRAAGATKNALAGAMNASAVLLFVASPQVHWTPAAVLGAGAILGGLAGAWALR
                     RVNERLLRAAIVVLGLALTAGLFLRPI"
     gene            1465..>2035
                     /locus_tag="VF453_12420"
     CDS             1465..>2035
                     /locus_tag="VF453_12420"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HEX7688508.1"
                     /translation="MKTKTLAVLAAFACIGGTASAATNVHAWTDTDYGVYSDNLVLGP
                     QLSYHIVTLANPGGDSGASASFDDAWTFTLSDPTDLDSYAATWQVKDTNISSATFALY
                     SGMPGSGSVVFSSSVDNMGESFSNKDLKAGDYYFEISGTYSTKYGYYEIDATANNAGG
                     PSAVPEPANAALLFAGLGMIGLVAKRRRSP"
BASE COUNT          337 a          741 c          699 g          258 t
ORIGIN      
        1 gacggctgcc tcgcgttcgc gcgcgagcac cgcgtcgtcg tcgagcccgc ctgcggcgcg
       61 agcatcgccg cgacgctggc cggcggcgcc gcgttcgcgg acgcgcgcac ggtgctggtg
      121 gtcgcctgcg gcggcgtctc gtcgacctac gagcaactgc aggcctggcg cgacgcgttc
      181 gcgcgcgacg ggttggcagg ctagaaccga cgttcgcctg gtgtcgtgat cgagaagttc
      241 gttgaacagg tctcgagcgc agaggcggcg tcgctgaatt ctttcgcaat cgggcgcaga
      301 ctaggcgccg cgcgcagcga ggctcgatgc ctcgcgagca cggcaacgac ggctgcgatc
      361 gatttcggaa gaatgcttca acgaacttct cgatcacgac cctagatcgg ccgcaggaac
      421 aggccggcgg tcagcgccag ccccagcacg acgatcgccg cgcgcagcag gcgctcgttg
      481 acgcggcgca gcgcccaggc ccccgccagg ccgccgagga tcgcgcccgc gcccagcacc
      541 gcggccggcg tccagtgcac ctgcggcgag gcgacgaaca gcagcaccgc cgacgcgttc
      601 atcgcgccgg cgagcgcgtt cttggtcgcg ccggctgcgc gcgtcgcgag gccggccatc
      661 gtgagcgcgg ccagcatcag gaagccgatg ccgccgccga agtagccgcc gtagatcgcg
      721 atcaggaact gcgcgatgcc ggcgcccacg ggcccgaggt gcgccgcccc ctcgaccggg
      781 cggcgccgga agctgcccca ggcgaacacg gcggtcgcga acagcacgag ccagggcacg
      841 agccgcgcga agatcgtcgc gggcgtgttc agcagcagca ggccgcccac cgccccgccg
      901 gcgaggctga tcgcgagcag cacgcgaaac gacaggccgc ggacgccctc caccagccgc
      961 cggcccgcga cgcccgcagt cacctggccg gggaacagcg cgacggtcga cgtgatgttg
     1021 gccgccagcg gcgtgagccc cgcgaggatc agcgccggga gcgtcacgaa cgagccgccg
     1081 ccggccagcg tgttctgcag cccggcccac agtccggcga cgaagacgag cacgagcagc
     1141 cccatgggcg agcaggttag cacccgtccg gaggtgtgcc ggggaagcga cggccgcggc
     1201 cagcggtcgg cccgtccgcg atgcccgtag ccaggccggc gacggcggga tcggtacagg
     1261 taaccgatgg caactgcagt ccagtagccc gtgcggtgcg ccgtaccgac gaacgcgggg
     1321 ggtgagcccc ctaggtcagg gcatggccat ccgccgtgaa tttggtcacg atctgtcacg
     1381 cactcagcgg tggcatgtcc gttgccaacc accgtccggg ccgcgccggc ccctccatct
     1441 gaacatcaac tcgaggaaga cagcatgaag acgaagactc tcgcagtcct ggccgcattc
     1501 gcctgcatcg gaggcaccgc ttccgcggcg accaacgtcc acgcctggac ggacaccgac
     1561 tacggcgtgt acagcgacaa cctggtgctg ggcccgcaac tgagctacca catcgtgacg
     1621 ctcgcgaacc cgggcggcga ctcgggcgcc tcggcgtcgt tcgacgacgc gtggacgttc
     1681 accctctccg acccgacgga cctggactcg tacgccgcca cctggcaggt caaggacacg
     1741 aacatctcgt cggcgacctt cgcgctgtac tccggcatgc cgggttccgg ctcggtggtg
     1801 ttctcgtcgt ccgtcgacaa catgggcgag agcttctcga acaaggacct caaggcgggc
     1861 gactactact tcgagatctc gggcacctac agcaccaagt acggctacta cgagatcgac
     1921 gccaccgcca acaatgccgg cggcccctcg gccgtcccgg agcccgcgaa cgccgcgctg
     1981 ctgttcgccg gcctcggcat gatcgggctg gtggccaagc gccgtcgttc cccgc
//
LOCUS       DASVLN010000505         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_80511, whole genome shotgun sequence.
ACCESSION   DASVLN010000505 DASVLN010000000
VERSION     DASVLN010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80511"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VF453_12425"
     CDS             complement(<1..195)
                     /locus_tag="VF453_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HEX7688509.1"
                     /translation="MAMTCKGIAGATAGCNAASCEDGAVGAEAAATAVAALAAAIGAV
                     TGAEETLVPAATGAVVATVAV"
     gene            179..406
                     /locus_tag="VF453_12430"
     CDS             179..406
                     /locus_tag="VF453_12430"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HEX7688510.1"
                     /translation="MQVIATADSWVEVRDAQGRTLLSRTVVAGESVGLDGAMPMHVKV
                     GNARGTRLTLRGDNVDLAPWTRDNVARLELK"
     gene            417..1733
                     /gene="ispG"
                     /locus_tag="VF453_12435"
                     /gene_synonym="gcpE"
     CDS             417..1733
                     /gene="ispG"
                     /locus_tag="VF453_12435"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX7688511.1"
                     /translation="MTDTTLPDRAPDVPPCALEGEPIPEAAPAPRRSRQARVVWDGNV
                     VTVGGDAPVRVQSMTNTDTVQVIETAIQVKELALAGSELVRITVNTPEAAAAVPHIRE
                     QLDRMGIRVPLVGDFHYNGHRLLTEYPACAEALSKYRINPGNVGRGEKGDRQFAQMVE
                     VACKHGKPVRIGVNWGSLDQELLKKLMDDNARRPEPWDIKHVMYEALVRSAIESAEAA
                     RDIGLDPNQILLSCKMSGVQDLVSVYRALSKRCDYALHLGLTEAGMGAKGTVASAAAL
                     AILLQEGIGDTIRVSLTPQPGEARTQEVVVALEILQSLGLRKFNPSVTACPGCGRTTS
                     TVFQDLTKQIDDFLRAQMPVWRDRFPGVESMKVAVMGCIVNGPGESKHADIGISLPGT
                     GEAPSAPVYIDGERVLTLRGDNIAAEFQAIVEQYIERRYGAATTAH"
     gene            1815..3155
                     /gene="hisS"
                     /locus_tag="VF453_12440"
     CDS             1815..3155
                     /gene="hisS"
                     /locus_tag="VF453_12440"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX7688512.1"
                     /translation="MAEKLRAVKGMNDIFPASVPRKADETLPSSNAWQWFETVARSVL
                     QRYGYQNLLTPVVEPTALFVRGLGEVTDIVEKEMYTFRDSMNGDQLTLRPEATAGIVR
                     AMIEHNALYNGPLRLWTMGPIFRHERPQKGRYRQFHQLDVEALGFAGPDVDAEQILLT
                     RALLRELGMKEGEDVRLELNSLGQPAERNAHRAALIAYFEANADQLDEDAKRRLHTNP
                     LRILDTKNPAMQALVNAAPRLMDFLGAESLAHFEAVKAVLDAAGLAYTVNTRLVRGMD
                     YYNLTVFEWVTDRLGSQGTVCGGGRYDGLIEQLGGKPAPAVGWGLGIERLLLLLEAIG
                     IQPPSDAPDVYAVLATDAARVPAMIAAEALRAAGLAVVVHAQGKEGLPSMKSQFKKAD
                     ASGARFALVFGEDELARGAVAIKPLRSDAGGEGAQQVERALADVAAWAAGLRKA"
     gene            3247..>3874
                     /locus_tag="VF453_12445"
     CDS             3247..>3874
                     /locus_tag="VF453_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7688513.1"
                     /translation="MAKQLDLQEQEQIDALKAFWTTYGNLITWALTIVLAAFAGWNWW
                     LYHQRQEAITASSMYGEIEAANAAGDATRAAHVFSDMKDKVGKTTYAQLGGLLAAKVQ
                     QDKGQAADAIASLQWVAEHGDAENAALARLRLSGLLADGKKYDDALAQLDKVTLPAFA
                     ALVADRRGDILAAKGQPDGAKAAWKAAWDGLPATTDYRQVVEAKLAAVG"
BASE COUNT          609 a         1408 c         1360 g          497 t
ORIGIN      
        1 gacagcgacc gtcgcgacca cggccccggt cgcggcgggc acgagcgtct cctccgcgcc
       61 ggtgacggcg ccgatcgccg cggccagcgc cgccactgcg gtcgccgccg cttccgcccc
      121 gacggcgccg tcctcgcaag acgcggcgtt gcatccggcc gtcgcgccgg cgatcccctt
      181 gcaggtcatc gccacggccg actcgtgggt ggaggtgcgc gacgcccagg gccggacgct
      241 gctgtcgcgc acggtggtgg cgggcgagtc ggtcggactc gacggcgcga tgcccatgca
      301 cgtcaaggtc ggcaacgcgc gcggcacgcg gctgacgctg cgcggcgaca atgtggatct
      361 cgcgccctgg acgcgcgaca acgtggctcg cctcgagctc aagtgacgac cccgacatga
      421 ccgacacgac cctgcccgac cgcgcccccg acgtgccccc ctgcgcgctg gaaggcgagc
      481 ccatccccga ggcggcgccc gcgccgcgcc gctcgcgcca ggcgcgcgtc gtctgggacg
      541 gcaacgtcgt caccgtgggt ggcgacgctc cggtgcgcgt gcagtccatg acgaacacgg
      601 acaccgtgca ggtcatcgag accgcgatcc aggtcaagga gctcgcgctg gcgggctccg
      661 agctcgtgcg catcaccgtc aacacgccgg aggccgccgc cgcagtgccg cacatccgcg
      721 agcagctcga ccggatgggc atccgcgtgc cgctggtggg cgacttccac tacaacggcc
      781 accgcctgct gaccgagtac ccggcctgcg ccgaggcgct gtcgaagtac cgcatcaatc
      841 ccggcaacgt cggccgcggc gagaagggcg accgccagtt cgcgcagatg gtcgaggtcg
      901 cgtgcaagca cggcaagccg gtgcgcatcg gcgtgaactg gggctcgctc gaccaggagc
      961 tgctcaagaa gctgatggac gacaacgccc gccggcccga gccctgggac atcaagcacg
     1021 tgatgtacga ggcgctcgtg cgctcggcga tcgagtcggc cgaggcggcg cgcgacatcg
     1081 gcctcgaccc gaaccagatc ctcctgagct gcaagatgag cggcgtgcag gacctggtct
     1141 cggtctaccg cgccctctcg aagcgctgcg actacgcgct gcacctgggc ctgaccgagg
     1201 ccggcatggg tgccaagggc accgtggcct cggccgcggc gctggcgatc ctgctgcagg
     1261 agggcatcgg cgacacgatc cgcgtctcgc tcacgccgca gccgggcgag gcgcgcacgc
     1321 aggaggtcgt ggtggcgctg gagatcctgc agtcgctggg cctgcgcaag ttcaacccca
     1381 gcgtcacggc ctgcccgggc tgcggtcgca cgaccagcac cgtcttccag gacctcacca
     1441 agcagatcga cgacttcctg cgcgcgcaga tgcccgtctg gcgcgaccgc ttcccgggcg
     1501 tcgagtcgat gaaggtcgcg gtgatgggct gcatcgtcaa cggcccgggc gagagcaagc
     1561 acgccgacat cggcatcagc ctgcccggca ccggcgaggc gccatccgcg ccggtctaca
     1621 tcgacggcga gcgcgtcctg acgctgcgcg gcgacaacat cgccgccgag ttccaggcga
     1681 tcgtcgagca gtacatcgaa cggcgctacg gggcggcgac gacggcgcat tgacgccgtc
     1741 ttccgcatcc cgcgtcccac cacgatcaac ccgcggccgc cacgcgcggc cgcccgatgc
     1801 gtattgctcc atccatggct gaaaaactgc gtgccgtcaa aggcatgaac gacatcttcc
     1861 cggcgtccgt gccgcgcaag gccgacgaga cgctgcccag ctcgaacgcc tggcagtggt
     1921 tcgagacggt cgcgcgctcg gtgctgcagc gctacggcta ccagaacctg ctcacgccgg
     1981 tcgtcgagcc gacggcgctg ttcgtgcgcg ggctgggcga ggtcaccgac atcgtcgaga
     2041 aggagatgta caccttccgc gattcgatga acggcgacca gctgacgctg cgccccgagg
     2101 cgacggccgg catcgtgcgc gcgatgatcg agcacaacgc gctctacaac ggccccctgc
     2161 gcctgtggac gatgggtccg atcttccgcc acgagaggcc gcagaagggc cgctaccgcc
     2221 agttccacca gctcgacgtc gaggcgctcg gcttcgccgg ccccgacgtg gacgcggagc
     2281 agatcctgct gacccgcgcc ctgctgcgcg agctcgggat gaaggagggc gaggacgtgc
     2341 ggctggagct caacagcctc ggccagcccg cggagcgcaa cgcccaccgc gccgcgctca
     2401 tcgcctactt cgaggcgaac gccgaccagc tcgacgagga cgcgaaacgg cgcctgcaca
     2461 cgaacccgct gcgcatcctc gacaccaaga acccggccat gcaggcgctc gtgaacgccg
     2521 cgcccaggct gatggacttc ctcggcgcgg aatcgctcgc gcacttcgag gcggtcaagg
     2581 cggtgctcga tgccgcaggg ctggcctaca cggtcaatac gcgcctcgtg cgcggcatgg
     2641 actactacaa cctgaccgtc ttcgaatggg tcacggaccg cctcggttcg cagggcacgg
     2701 tctgcggggg cgggcgctac gacggcctga tcgagcagct gggcggcaag cccgcgccgg
     2761 cggtcggctg gggcctgggc atcgagcgcc tgctgttgct gctcgaggcg atcggcatcc
     2821 agccgccctc ggacgcgccc gacgtctatg ccgtgctcgc gaccgacgcc gcgcgcgtgc
     2881 ccgcgatgat cgccgccgag gcgctgcgcg cggccggcct cgcggtcgtc gtgcacgcgc
     2941 agggcaagga gggcctgccg tccatgaagt cgcagttcaa gaaggccgac gccagcggcg
     3001 cgcgcttcgc gctggtgttc ggcgaggacg agctcgcgcg cggcgcggtg gcgatcaagc
     3061 cgctgcgatc ggatgcgggc ggcgagggcg cgcagcaggt cgagcgagcg ctcgcggacg
     3121 tcgcggcctg ggccgcgggg ctgcgcaaag cataatcggg tgttttcgtc cgccgccttg
     3181 gcggacccgc ctctccctac cgagcccgcg cccgcgggcg caccccgttt ccgacctttc
     3241 ccaccgatgg caaagcaact cgatctccag gaacaagaac agatcgacgc cctgaaggcg
     3301 ttctggacga cctacggcaa cctcatcacg tgggcgttga cgatcgtgct ggccgccttc
     3361 gcgggctgga actggtggct ctaccaccag cgccaggagg cgatcaccgc cagctcgatg
     3421 tacggcgaga tcgaggccgc caacgcggcc ggggacgcga cgcgcgcggc ccacgtgttc
     3481 tcggacatga aggacaaggt cggcaagacg acctacgcgc agctgggcgg gctgctcgcg
     3541 gccaaggtgc agcaggacaa ggggcaggcc gccgacgcga tcgcgagcct gcagtgggtc
     3601 gccgagcacg gcgacgccga gaacgcggcg ctcgcgcgcc tgcgcctctc gggcctgctg
     3661 gccgacggca agaagtacga cgacgcgctc gcgcagctcg acaaggtgac gctgcccgcg
     3721 ttcgcggcgc tggtcgccga tcgccgcggc gacatcctcg ccgccaaggg ccagcccgac
     3781 ggcgccaagg cggcctggaa ggcggcctgg gacggcctgc ccgccaccac cgactaccgc
     3841 caggtggtcg aggccaagct ggccgcggtg ggcg
//
LOCUS       DASVLN010000506         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_80604, whole genome shotgun sequence.
ACCESSION   DASVLN010000506 DASVLN010000000
VERSION     DASVLN010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80604"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1888
                     /locus_tag="VF453_12450"
     CDS             <1..>1888
                     /locus_tag="VF453_12450"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX7688514.1"
                     /translation="FEHLGRGDGLPNEIATAVEEDDQGFLWVGTEEGLARWDGYRFRV
                     YGADAGDPRRLAAGFVRALRRDAQGRLWIGTDSGGLARLDPADGGFTRVGGNLSVVGL
                     AADRDGGMWVATTTGLLHVLSGDVVEQPWRHDAGTPEALPDPSVTAVLQRRSGDVWIG
                     MAASLVVRHPDGRPGRTVELPGIEAGTPAGVNALYEDIDGRVWIGTALHGAYVWDPAD
                     AQVHRLRAMPGARAGEPPLDSDSVTAFAESAPGEVWVGTATRGLLVVEARTHVARRVM
                     QDRAVPTGLQSNWVWALHRDRSGQMWIGSTSGLSRTRPREDAVLAIVPSEARGWSDPS
                     VLAVEAAPDGVLTLGQHGGLVTVDPFARRITPLRARSQGEDDSPVFGYISAIARTADG
                     RLFLGAKEGLFVRLPGAPRLQEWTLPQRQRVKGVNALLCDGQTLWIGGKFDGVWTMDL
                     ASAAHVVARPDVRGQLTDPQVNTLARAPDGAIWVGTKNGLNRLVPGQAIEQILSTPGD
                     PATLPDGVVSALLFDRQGRLWVATNAGLAVMERRDGAGRPVFRRLGLAQGLTSLNVDK
                     LLLDAQGRVWASTDDGLLVVDPATLAVRALHAAEGVAISTYWANSGAASADGSLLFFG
                     GLGGL"
BASE COUNT          215 a          706 c          725 g          242 t
ORIGIN      
        1 tcttcgagca cctcggccgc ggcgacggcc tgcccaacga gatcgcgacg gcggtcgagg
       61 aggacgacca gggcttcctg tgggtcggca ccgaggaggg cctcgcgcgc tgggacggct
      121 accgcttccg cgtctacggc gccgacgccg gcgacccgcg ccgcctcgcc gccggcttcg
      181 tgcgcgcgct gcggcgcgac gcccagggcc gactgtggat cggcaccgac tccggcggcc
      241 tcgcccgcct ggatccggcc gacggcggct tcacacgcgt cggcggcaac ctctcggtgg
      301 tcgggctggc ggccgatcgc gacggcggca tgtgggtcgc gacgaccacc ggcctgctgc
      361 acgtgctgtc cggcgacgtg gtcgaacagc cctggcgcca cgacgccggc acgcccgagg
      421 cgctgcccga tccgagcgtc acggccgtcc tgcagcgccg ttcgggcgac gtctggatcg
      481 gcatggccgc cagcctcgtg gtgcgccatc ccgacggccg ccccggccgg acggtcgagc
      541 tgcccggcat cgaggccggc acgccggcgg gcgtcaacgc gctgtacgag gacatcgacg
      601 gccgcgtctg gatcggtacc gcgctgcacg gcgcgtacgt ctgggatccg gccgacgccc
      661 aggtgcaccg cctgcgcgcc atgccgggcg cgcgcgccgg cgagccgccg ctggacagcg
      721 actccgtcac cgcgttcgcc gagtcggcgc ccggcgaggt ctgggtcggc acggcgacgc
      781 gcgggctgct ggtcgtcgag gcgcgcacgc acgtcgcgcg ccgcgtgatg caggaccgcg
      841 cggtgcccac gggcctgcag tcgaactggg tctgggcgct gcaccgcgac cgcagcggcc
      901 agatgtggat cggctcgacc agcggcctca gccgcacgcg gccgcgagag gacgcggtgc
      961 tcgcgatcgt gccctccgag gcgcgcggct ggtcggatcc gtcggtgctg gcggtcgagg
     1021 ccgcgccgga cggcgtgctc acgctgggcc agcatggcgg gctcgtcacg gtcgacccgt
     1081 tcgcgcgccg catcacgccg ctgcgcgcgc ggtcgcaggg cgaggacgac tcgcccgtgt
     1141 tcggctacat cagcgcgatc gcgcgcacgg cggacgggcg cctgttcctg ggcgccaagg
     1201 agggcctgtt cgtgcgcctg cccggcgcgc cgcggctgca ggagtggacg ctgccgcagc
     1261 gccagcgggt caagggcgtc aatgcgctgc tgtgcgacgg ccagacgctg tggatcggcg
     1321 gcaagttcga cggcgtctgg acgatggacc tggcctcggc cgcgcacgtc gtcgcgcggc
     1381 cggacgtccg cggccagctc accgatcccc aggtcaacac cctggcccgc gcgcccgacg
     1441 gcgcgatctg ggtcggaacg aagaacggcc tgaaccggct cgtgcccggg caggcgatcg
     1501 agcagatcct ctcgacgccg ggcgatccgg ccacgctgcc ggatggcgtc gtctcggcgc
     1561 tgctgttcga ccgccagggt cggctgtggg tcgcgaccaa cgcgggcctg gccgtgatgg
     1621 agcggcgcga cggcgcgggc cggccggtgt tccgccgcct gggcctcgcg caggggctca
     1681 ccagcctgaa cgtcgacaag ctgctgctgg acgcccaggg ccgcgtctgg gcgagcaccg
     1741 acgacggcct gctggtggtc gacccggcga cgctcgccgt gcgcgcgctg cacgccgccg
     1801 agggggtcgc gatctcgacc tactgggcca actccggcgc cgcctcggcc gacggctcgc
     1861 tgctgttctt cggcggcctc ggcggcct
//
LOCUS       DASVLN010000507         8705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_240599, whole genome shotgun sequence.
ACCESSION   DASVLN010000507 DASVLN010000000
VERSION     DASVLN010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240599"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VF453_12455"
     CDS             <1..283
                     /locus_tag="VF453_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7688515.1"
                     /translation="LTPSQRWRAIDLPLAAPVLMAGIKTAAVITVGTATIAAFVGAGG
                     LGERIVTGLALNDHALLLAGALPAAALALAAQGLFGLLEWWVRPRRATA"
     gene            complement(302..1699)
                     /gene="gorA"
                     /locus_tag="VF453_12460"
     CDS             complement(302..1699)
                     /gene="gorA"
                     /locus_tag="VF453_12460"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX7688516.1"
                     /translation="MATTHDLIVIGGGSGGVAGARRAAALGAKVAIVEADRVGGTCVI
                     RGCVPKKLMMYAAGFAREFEQARGFGWSDVEARFEMARWAEAKAREIGRLEHVYDEML
                     LNAGVELVRGRARLASACEVAVGERTLSAPRILVATGGSPARDAFAGLELAMTSNEVL
                     DLRELPATLLVIGGGYIAVEFASILAALGVRVTIAYRDVHPLRGFDTELRGRLAMALA
                     DRGITLAAGVGFERLERDAGGFALRRVDGTVLQAEAALNATGRTPNTRDLGLERVGVR
                     LTARGAIAVDADSRASVSGIWAVGDVTDRVNLTPVAIAEARAFAETEFGGAPTRIDHR
                     TVASAVFTDPSIGTVGLSEQQAALHGPVDVYGADFRAMKTAFVGGTQRSYMKLVVDGL
                     SQRVLGVHMIGPDAPEIVQGLAVAVTCGARKSDFDRTIAVHPTLAEEFMLMRTPVRRV
                     AQTVQPPPAQPAAMA"
     gene            complement(1771..2730)
                     /locus_tag="VF453_12465"
     CDS             complement(1771..2730)
                     /locus_tag="VF453_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688517.1"
                     /translation="MLACAGLLAMLSACGGGGDGSPAAGASALGVERGGSLQAPLRVF
                     VPDTGRRGIVGLAKLEPDAGQALARPVVSLTATPGENVQLDRARDELYAIAGRTVLVY
                     AHASLLRPDALPARSFTLPDTLVAPHALFLDTARDELYVGGDTAHGSGEIVAYPYAHA
                     LRGSPATPSRALFVDHGVSFFTIDPVRERLYVVNADAGVHVFASVDTASGPQQAVATV
                     PVLGSGLAVDPTRDRLYVADMFAGLILVDHASAPAPVVRATVSIDDAHQVAVDPARDR
                     VYVSALGALYTLDEASALTDASTVNQPAVAGSPSAMLGAAAVR"
     gene            complement(3112..3441)
                     /locus_tag="VF453_12470"
     CDS             complement(3112..3441)
                     /locus_tag="VF453_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688518.1"
                     /translation="MVSVDEALSVDEFDDELLALLSVELLFMLLDDEVDDVDDAPLFS
                     ELLPALSLLFIELAFASAGEVAAGAPVADEPSDDDDEDWAQATPITAAMAAEAAVTTS
                     LVWKLRM"
     gene            complement(3533..4153)
                     /locus_tag="VF453_12475"
     CDS             complement(3533..4153)
                     /locus_tag="VF453_12475"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEX7688519.1"
                     /translation="MPSDVAPEPASRPRCAACALPLATCLCALVVRVDNRVPLLVLQH
                     PREAREAKGSARLLARCLAQCRVVVGEEFDDATLAPLLHGGGRHSVLLYPPDDGAAGM
                     IVDADAVRDDASLQLVAIDATWRKSLRLLRAHPMLAALPRITLPPGEASRYAALRKAR
                     RPGQLSTLEASCGALALLERSPARYVPVLDAFERFVADRVARAARP"
     gene            4228..4734
                     /locus_tag="VF453_12480"
     CDS             4228..4734
                     /locus_tag="VF453_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7688520.1"
                     /translation="MDHDKDAADRQARLFALIKDVKFAMFTTHTASGHLHSRPMTTLN
                     TRIDEDDALWFFMSRAGAPVAEMGNDTQVNVSYANPSDDAYVSVTGVARVVEDLARKQ
                     ALWTPAAQAWFSGVDDPDLALVEVHIEHADYWNVRDNKLVQLYKRAEAALSGKQPNLA
                     EHGRVRPH"
     gene            complement(4840..5622)
                     /locus_tag="VF453_12485"
     CDS             complement(4840..5622)
                     /locus_tag="VF453_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7688521.1"
                     /translation="MTTASTSPDTARIALITGGSRGLGRNAALKLAAEGVDVILTYHR
                     NEAEAHAAVREIEVLGRRAVALQLDVGSTGGFPAFVKRVRDALAATWRRERFDYLVNN
                     AGSGVHASIEQTTEAQFDQLVDVHLKGTFFLTQQLLPLIEDGGRIVNTSSGLVRFTYP
                     GYAAYAMMKGGIEVLTRYLAKELGPRRIAVNTIAPGAIETDFGGGTVRDNVELNRAIA
                     SQTALGRVGLPDDIGGAVAALLADGTGWINGQRIEVSGGIHL"
     gene            5733..6656
                     /locus_tag="VF453_12490"
     CDS             5733..6656
                     /locus_tag="VF453_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7688522.1"
                     /translation="MRDPLELESLRVFAKVAELASFTRAAEQLGLAKGHVSTSVRALE
                     ERIGTRLLQRTTRQVRITPDGERFLDRAKELLAEAEQLDAMFRPAATGLAGRLRIDLP
                     TALARDIVVPRLPEFLAAHPQLEIGLSTTDRRVDLVHEGFDVVLRVGALGDSDLVARP
                     LGHLRMVNAASPAYLREHGTPRTLDDLAGHRVVHYASTLSHAGASWEYRDPEDGTWRT
                     RPMRAAIVVNGTDAYQAAALAGLGLYQAPRTGVSRLVREGRLVEVLRDYTAAPLPVSL
                     LYPNRRHLAPRVQAVLAWLEEIVRPYLDEAG"
     gene            6782..7417
                     /locus_tag="VF453_12495"
     CDS             6782..7417
                     /locus_tag="VF453_12495"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX7688523.1"
                     /translation="MHARLAIALLAAATLGHCGPARAAEQARTFAPFTAVANGGPVSL
                     DIEVGKPQSVTVTGNDRFLADLRTDVVDGQLRIELPDHDHGSAYGHPHVTITVPRLER
                     FEMSGAGATVITHMAGDRLDVRYSGAGSLHADGQVKDLRLDVSGVGSIDARELHAQWA
                     EVRVSGVGSVRVHASERLDASVSGIGSLAYYGHPKSVETHGGGLGSIGPGH"
     gene            complement(7428..7991)
                     /locus_tag="VF453_12500"
     CDS             complement(7428..7991)
                     /locus_tag="VF453_12500"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7688524.1"
                     /translation="MTAPSLPTLSTARLSLRPTGMDDVPALFELFSDPRVTRYGSRPP
                     WTDRRQAVEQVERDLVALAQGEAVRFAMLRCGDDRLIGTCTLFHFDAECRRAEVGYML
                     SVAAWGQGYASEAVEAMLDWGFTARGLNRVEADIDPRNEASARLLERLGFTREGHLRE
                     RWIAGGEKSDSWLYGLLASEWASRPWR"
     gene            complement(8416..>8705)
                     /locus_tag="VF453_12505"
     CDS             complement(8416..>8705)
                     /locus_tag="VF453_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688525.1"
                     /translation="LLRRHRPALAWGVDDFEDHVADGEESAGRVVVLDPALPADARDP
                     LVFDALAVAWTRLQAMALGQDGFFTFADELARQLHQGNGFPSLERAGDLPG"
BASE COUNT         1260 a         3236 c         3081 g         1128 t
ORIGIN      
        1 cctcacgccg tcgcagcgct ggcgcgcgat cgacctgccg ctggccgcgc ccgtgctgat
       61 ggccggcatc aagacggcgg cggtgatcac ggtcggcacc gcgaccatcg ccgccttcgt
      121 cggcgccggc ggcctgggcg agcgcatcgt gaccggactc gcgctcaacg accacgcgct
      181 gctgctggcc ggggcgctgc cggcggccgc cctggcgctg gccgcgcagg gcctgttcgg
      241 cctccttgaa tggtgggtgc ggccacgccg ggccacggcg tgagcgatac ccgatgcggt
      301 atcaggccat ggccgccggc tgcgcgggcg gcggctgcac cgtctgggcg acgcgccgca
      361 ccggggtgcg catcagcatg aactcctcgg ccagcgtcgg atggacggcg atcgtgcggt
      421 cgaagtcgga cttgcgcgcc ccgcaggtca ccgcgaccgc caggccctgc acgatctcgg
      481 gcgcgtcggg gccgatcatg tgcaccccca gcacccgctg gctgaggccg tcgacgacca
      541 gcttcatgta gctgcgctgc gtgccgccga cgaaggccgt cttcatcgcg cggaagtcgg
      601 ccccgtacac gtccacgggt ccgtgcagcg cggcctgctg ctcgctcagg ccgacggtgc
      661 cgatcgacgg gtcggtgaac acggcgctgg cgacggtgcg gtggtcgatg cgcgtcggcg
      721 cgccgccgaa ctcggtctcg gcgaaggcgc gcgcctcggc gatcgccacg ggcgtcaggt
      781 tgacgcggtc ggtcacgtcg ccgaccgccc agatgcccga gacgctcgcg cggctgtccg
      841 cgtcgaccgc gatcgcaccg cgcgccgtga gcctgacgcc gacccgctcc aggccgagat
      901 cgcgcgtgtt gggcgtgcgg ccggtcgcgt tgagcgccgc ctcggcctgc agcaccgtgc
      961 cgtcgacgcg ccgcagcgcg aagccgcccg cgtcgcgctc cagccgctcg aagcccacgc
     1021 ccgcggccag cgtgatcccg cgatcggcga gcgccatcgc gaggcggccg cgcagctcgg
     1081 tgtcgaagcc gcgcagcggg tgcacgtcgc ggtaggcgat ggtcacccgc acgcccagcg
     1141 cggcgaggat gctcgcgaac tccaccgcga tgtagccgcc gccgatcacc agcagcgtcg
     1201 ccggcagctc gcgcaggtcg agcacctcgt tggaggtcat cgccagctcg aggccggcga
     1261 aggcgtcgcg cgccggcgag ccgcccgtcg cgacgaggat gcgcggcgcc gacagcgtgc
     1321 gttcgccgac cgccacctcg cacgccgacg cgagccgcgc ccgtccgcgc accagctcca
     1381 cgccggcgtt cagcagcatc tcgtcgtaca cgtgctccag gcggccgatc tcgcgcgcct
     1441 tggcctcggc ccagcgcgcc atctcgaagc gcgcctcgac atcgctccag ccgaagccgc
     1501 gcgcctgctc gaactcgcgg gcgaagccgg cggcgtacat catgagcttc ttcggcacgc
     1561 agccgcggat cacgcaggtg ccgcccacgc gatccgcctc cacgatggcc accttcgcgc
     1621 ccagtgccgc cgcacggcgc gcgcccgcca cgccgccgga gccgccgccg atcacgatca
     1681 ggtcatgggt cgtcgccatc gaatgagtcc cgcgagtctt gcgaaggcga catgctcgca
     1741 gacttcgccg cttcccgcga gcggcggggc ctaacgcacc gcggccgcgc ccagcatcgc
     1801 cgacggcgag cccgcgaccg cgggctggtt gacggtcgac gcatcggtga gcgcgctcgc
     1861 ctcgtcgagc gtgtacagcg cgccgagcgc cgagacgtag acgcggtcgc gggccggatc
     1921 cacggcgacc tggtgcgcgt cgtcgatgct caccgtggca cgcacgacgg gcgcgggcgc
     1981 gctcgcgtgg tcgacgagga tcagccccgc gaacatgtcg gcgacgtaga gccggtcgcg
     2041 cgtcggatcg accgccaacc ccgagcccag caccggcacg gtggccaccg cctgctgcgg
     2101 gccgctggcg gtgtccaccg acgcgaacac gtggacgccg gcatcggcgt tgacgacgta
     2161 cagccgctcg cgcaccgggt cgatcgtgaa gaacgacacg ccgtggtcga cgaacagcgc
     2221 gcgcgacggc gtcgcgggcg agccgcgcag cgcatgcgcg tacgggtagg cgacgatctc
     2281 cccgctgccg tgcgccgtgt cgccgccgac gtagagctcg tcgcgcgcgg tgtcgaggaa
     2341 cagggcatgc ggcgcgacca acgtgtcggg cagcgtgaag ctgcgcgccg gcagggcgtc
     2401 gggccgcagc aggctcgcat gcgcgtagac gagcacggta cggccggcga tcgcgtagag
     2461 ctcgtctcgc gcgcggtcca gctgcacgtt ctcgcccggc gtcgccgtca acgacacgac
     2521 aggccgcgcc agcgcctgcc ccgcatcggg ctcgagcttg gccagcccga cgatcccgcg
     2581 ccgccccgta tcgggcacga agacgcgcaa cggcgcctgg aggctgccgc cgcgctccac
     2641 gcccagcgcg ctcgctccgg ccgccgggct gccgtcgcca ccgccgccac aagccgacag
     2701 catcgccagc aggccagcgc aggcgagcac gcaggtcgac ctcccgcgac gggggcgcgg
     2761 acgcggggcg gggcagggcg cgtgtcgcat cgcgatccgt gtcgaatttt ccaggggcgc
     2821 agtctgcaag gaagagattg ctcgacggtg aaccaatctt gtgccgccgg atacacgacc
     2881 gtaggtaaga aaccacgatg agctgcaaca cgacgtcacg gcgcggcgcg tgatacgtct
     2941 ggtcaccaag tcgtcatcga accccggacc gcgccgtacg cctctggagc ccggcgcacg
     3001 ggctttggtt ccccgcccga tacgcacggt ttctttacac gggtggcatt gccgttgccg
     3061 gaagaagagt cctggcctcg cacgggcgag accatcttcc aacaggagaa ctcacatgcg
     3121 cagcttccag accaaactcg tcgtgaccgc cgcttccgcc gccatggccg ccgtgatggg
     3181 cgtcgcctgg gcccagtcct cgtcgtcgtc gtccgacggc tcgtcggcga ccggcgcgcc
     3241 ggccgcgacc tcgcccgccg acgcgaacgc caactcgatg aacaacaacg acaacgccgg
     3301 caacaactcg ctgaacaacg gcgcgtcgtc cacgtcgtcc acctcgtcgt cgagcagcat
     3361 gaacaacagc tcgaccgaca acagcgccaa caactcgtcg tcgaactcgt cgaccgacaa
     3421 cgcctcgtcg acggacacca cgccggcgcc gcgttcggac cgcaactgac cgacctcgcg
     3481 tcgacccggc atcgccgccc atcccgtgca gggatcggcg gcgatgccgc tttcacgggc
     3541 gcgccgcgcg agcgacgcga tcggcgacga agcgctcgaa cgcgtccagc accggcacgt
     3601 agcgcgccgg cgatcgctcg agcagcgcga gcgccccgca gctcgcctcg agcgtgctca
     3661 gctggccggg ccggcgcgcc ttgcgcagcg cagcgtagcg gctcgcctcg ccaggcggga
     3721 gcgtgatccg cggcagtgcc gcgagcatcg gatgcgcgcg cagcaggcgc aggctcttgc
     3781 gccaggtcgc gtcgatcgcg acgagctgca gcgacgcgtc gtcccgcacg gcgtcggcat
     3841 cgacgatcat gcccgccgcg ccgtcatccg gcggatagag cagcacgctg tgccggccgc
     3901 cgccgtgcag caggggcgcg agcgtcgcgt cgtcgaactc ctcgcccacc acgacgcggc
     3961 actgcgccag gcagcgcgcg agcagccgcg cgctgccctt ggcctcgcgc gcctcccgcg
     4021 gatgctgcag cacgagcagc ggcacgcggt tgtcgacgcg cacgaccagc gcgcacaggc
     4081 aggtcgcgag cggcagcgcg caggcggcgc agcgcgggcg cgaggcaggc tcgggtgcga
     4141 cgtcggaagg catggagttc gaaggcggac gacgggcgcg gcccttgccg ccatgcccat
     4201 cgacgcacac cctacaggag tttcgacatg gatcacgaca aggatgccgc cgaccgccag
     4261 gcgcggctgt tcgccctcat caaggacgtg aagttcgcga tgttcacgac ccacaccgcc
     4321 tcgggccacc tgcactcgcg gccgatgacc acgctcaaca cgcgcatcga cgaggacgac
     4381 gcgctgtggt tcttcatgtc gcgcgccggc gcgcccgtgg ccgagatggg caacgacacg
     4441 caggtcaacg tgtcgtacgc gaacccgtcc gacgacgcgt acgtctcggt cacgggcgtc
     4501 gcccgcgtcg tcgaggatct cgcgcgcaag caggcgctgt ggactccggc cgcccaggcc
     4561 tggttcagcg gggtcgacga ccccgacctc gcgctggtcg aggtgcacat cgagcacgcc
     4621 gactactgga acgtgcgcga caacaagctc gtgcagctgt acaagcgcgc cgaggcggcg
     4681 ctgtccggca agcaaccgaa cctggccgag cacggacgcg tccgcccaca ctgagcgccg
     4741 gccccgcgcg ccgcgtgacg cgtgacgcgt gcgcgcgagg gctcgaacgg gcgcgggcac
     4801 caggcccgcg tccagcccct caccgatgcg ccggcggact cacaggtgga tgccgccgga
     4861 cacctcgatg cgctgcccgt tgatccagcc ggtgccgtcg gccagcagcg ccgcgacggc
     4921 gccgccgatg tcgtcgggca ggcccacgcg gccgagggct gtctgcgacg cgatggcgcg
     4981 gttgagttcg acgttgtcgc gcaccgtgcc gccgccgaag tccgtctcga tggcgcccgg
     5041 ggcgatcgtg ttgaccgcga tgcgacgcgg gccgagctcc ttggcgaggt agcgcgtcag
     5101 cacctcgatg ccgcccttca tcatcgcgta ggccgcgtag cccgggtacg tgaagcgcac
     5161 caggcccgac gacgtgttga cgatgcggcc gccgtcctcg atcagcggca gcagctgctg
     5221 agtcaggaag aacgttccct tcaggtggac gtcgacgagc tggtcgaact gcgcctcggt
     5281 cgtctgctcg atgctggcgt ggacgcccga gccggcgttg ttgacgaggt agtcgaagcg
     5341 ctcgcgccgc cacgtcgcgg caagcgcgtc gcgcacgcgc ttgacgaacg ccgggaagcc
     5401 gcccgtcgag ccgacgtcca gctgcagcgc gacggcgcgg cgaccgagaa cctcgatctc
     5461 gcgcaccgcg gcgtgcgcct cggcctcgtt gcggtggtag gtcaggatca cgtcgacgcc
     5521 ttcggcagcg agcttgagcg cggcgttgcg gccgaggccg cggctgccgc cggtgatcag
     5581 ggcgatgcgg gcggtgtcgg gggatgtcga agcagtggtc atggcgggct cctttgaagc
     5641 gggtggttga tcgatggagt gattctggga attcccgccg ctcggataaa gtcccggctc
     5701 gaggtcacac tgttccccac ggaagaacaa tcatgcgcga tcccctcgag ctcgaatcgc
     5761 tgcgcgtctt cgccaaggtg gcggagctcg cgagcttcac gcgcgccgcg gagcagctcg
     5821 ggctcgcgaa gggccatgtc tcgaccagcg tccgcgcgct cgaggagcgc atcggcacgc
     5881 gcctgctgca gcgcacgacg cgccaggtgc gcatcacgcc cgacggcgag cgcttcctcg
     5941 accgcgccaa ggagctgctg gccgaggccg agcagctcga cgcgatgttc cggcccgccg
     6001 cgacggggct cgccggccgg ctgcgcatcg acctgcccac cgccctcgcg cgcgacatcg
     6061 tcgtgccgcg gctgccggag ttcctcgccg cgcatccgca gctggagatc ggcctgagca
     6121 ccaccgaccg ccgcgtcgac ctcgtgcacg agggcttcga cgtcgtgctg cgcgtggggg
     6181 cgctgggcga ctccgacctc gtcgcccggc cgctcggcca cctgcgcatg gtcaacgccg
     6241 cgagcccggc atacctgcgc gagcacggga cgccgcggac gctggacgac ctcgcgggcc
     6301 accgcgtcgt gcactacgcg tcgacgctga gccacgccgg cgcgagctgg gagtaccgcg
     6361 acccggaaga cggcacctgg cgcacgcggc cgatgcgcgc ggccatcgtc gtcaacggca
     6421 ccgacgccta ccaggccgcc gcgctcgccg ggctcgggct gtaccaggcg ccgcgcacgg
     6481 gcgtctcgcg gctggtgcgc gagggccggc tggtcgaggt gctgcgcgac tacaccgccg
     6541 cgccgctgcc ggtctcgctg ctctacccga accggcgcca cctcgccccg cgggtgcagg
     6601 cggtgctcgc gtggctggag gagatcgtcc gcccctacct cgacgaggcg ggatgaggcc
     6661 ccgtccgtcg cgccggatcg cagcgcggca tcgcggcccg gcaggtcgtc gcacggggcc
     6721 acggagagcc ctcgccgccg cctagcatcc ttcccatcga caccaccgaa ggagacgccc
     6781 catgcacgcc cgcctcgcca tcgccctgct cgcggccgcc acgctcggcc attgcggccc
     6841 cgcccgcgcc gcggaacagg cgcgcacgtt cgcgccgttc actgccgtcg ccaacggcgg
     6901 gccggtcagc ctcgacatcg aggtcggcaa gccgcagtcg gtgacggtga cgggcaacga
     6961 ccgcttcctc gccgacctgc gcacggacgt ggtcgacggc cagctgcgca tcgagcttcc
     7021 ggatcacgac cacggcagcg cgtacggcca cccgcacgtc acgatcacgg tgccgcggct
     7081 cgagcgcttc gagatgtccg gcgccggcgc gaccgtgatc acgcacatgg ccggcgaccg
     7141 gctcgacgtg cgctactcgg gcgccggcag cctccacgcc gacgggcagg tcaaggactt
     7201 gcggctggac gtgagcgggg tcggctcgat cgacgcccgc gagttgcacg cgcaatgggc
     7261 cgaagtgcgc gtgagcggcg tcggctccgt gcgggtccac gccagcgagc gcctggacgc
     7321 ctcggtctcc ggcatcggct cgctcgcgta ctacggccac cccaagtcgg tcgagacgca
     7381 cggcggcggg ctgggcagca tcgggcccgg ccactgaccg cgccgcctca gcgccagggc
     7441 cggctcgccc attcgctcgc gagcaggccg tagagccacg agtcgctctt ctcgccgccg
     7501 gcgatccagc gctcgcgcag gtggccctcg cgcgtgaaac ccaggcgttc cagcaggcgt
     7561 gcggacgcct cgttgcgcgg gtcgatgtcc gcctccacgc gattgaggcc gcgcgccgtg
     7621 aagccccagt ccagcatcgc ctcgaccgcc tccgacgcat aaccctggcc ccaggccgcg
     7681 acggagagca tgtagccgac ctcggcgcgc cggcattccg cgtcgaagtg gaacagcgtg
     7741 caggtgccga tcagccggtc gtctccgcac cgcagcatcg cgaagcgcac ggcctcgccc
     7801 tgggccagcg ccacgaggtc gcgctcgacc tgttcgaccg cctggcggcg atcggtccac
     7861 ggcggccggc tgccgtagcg cgtgacgcgc ggatccgaga acagctcgaa caacgccggc
     7921 acgtcgtcca tgcccgtcgg tcgcagggac aggcgcgcgg tcgagagcgt cgggagcgag
     7981 ggggcggtca tgcgggtcgg cgtgatgcgg atcgggtcga gccggacggc cggctgcgaa
     8041 gggaacggat tctcacacga gggccgccgt gcgtgatgcg atcgtgcggc ggcgtggcgt
     8101 ggcgtcctcg cccggaaaaa ttccggcgtc ccttgttgtt ctcataagct tttcattctc
     8161 agggtggtgg cagtagtagg ggctgccgca tcctgtggac aaaggcggaa atccctggtc
     8221 catcaagcat ttgccgccgg aacaagcatg gggatcgcgc gcctcgttgc ggcaggacct
     8281 ttcgacaaca acttcgcgcg ccgccgccgg cctgtggacc gtggtgcagt tgtcccggaa
     8341 cggtccacat ccttgtgact tgacagcggc gatcgtcccg gtccggcggg cggcggcggc
     8401 gcgcgcggac gccggctagc ccgggaggtc gccggcgcgc tcgaggctcg ggaagccgtt
     8461 gccctggtgg agctggcgtg cgagctcgtc ggcgaaggtg aagaagccgt cctgcccgag
     8521 ggccatcgcc tgcagccgcg tccaggcgac cgccagggcg tcgaaaacca gcgggtcgcg
     8581 cgcgtccgca ggcagcgcgg gatcgagcac gaccacccgc ccggcgctct cctcgccgtc
     8641 ggcgacgtga tcctcgaagt cgtccacgcc ccacgcgagc gcaggacgat ggcggcgcag
     8701 cagct
//
LOCUS       DASVLN010000508         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_348174, whole genome shotgun sequence.
ACCESSION   DASVLN010000508 DASVLN010000000
VERSION     DASVLN010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348174"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VF453_12510"
     CDS             complement(<1..295)
                     /locus_tag="VF453_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688526.1"
                     /translation="MATIIRVDRAAGRAEHGRSSGANRMSADEVAIRIQGRCMTAKIG
                     ISRRRPSRTPYTPARRIDTPVAAAAAARERRAAQQRARKASARFSSSAAAAGSW"
     gene            370..1560
                     /locus_tag="VF453_12515"
     CDS             370..1560
                     /locus_tag="VF453_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7688527.1"
                     /translation="MTPSSSHPSAAEPSLSRRLGRLQATGLAAAAALSAWGASLAHSP
                     AVAAVVGAVLVAAGVALDRRRARRERHERDAAGAFVAGTDQLGQEVLPLWSAHIENSR
                     QQMETAIAALSQRFAGIVDRLDQTLRAQNDDGAGRGLASLFEHSDARLREVLGSLKEA
                     MASNGAMHEEVQSLGRFVAELQQMAAEVADIAQQTNLLAINAAIEAAHAGIEGRGFAV
                     LAQEVRKLSAMSGETGQRMAAKVAVIAQAIDAARASAEASAQREAASLGASDDAINEV
                     LGRFRDVTQAMESSATTLKHESEGIQSEIVEALVQLQFQDRVSQRMTHVRQNMERLPA
                     LLADSRRRFDASGELASVDARVLLDELEGSYAMVDERTTHAGEAGTPAAPAASASLEE
                     VTFF"
     gene            1610..1978
                     /locus_tag="VF453_12520"
     CDS             1610..1978
                     /locus_tag="VF453_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7688528.1"
                     /translation="MAKTIMIVDDSASLRQVVGIALRSAGYDVLEGCDGADALKKLTG
                     QKVHLIISDVNMPNMDGITLVKEVKKLAPYKFTPIVMLTTESADGKKAEGQAAGAKAW
                     VLKPFNPPQLLAVVQKLVQP"
     gene            2032..2337
                     /locus_tag="VF453_12525"
     CDS             2032..2337
                     /locus_tag="VF453_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7688529.1"
                     /translation="MTATPFRIDGEFTIYRAAELAEGLKAALAATPAGHALEIDLSEV
                     TEMDSAGVQLLLAARRGAAQGGRSLRLSGRSPAVAEVFATLRLDAHFDAAAATAQAE"
     gene            2349..>2733
                     /locus_tag="VF453_12530"
     CDS             2349..>2733
                     /locus_tag="VF453_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX7688530.1"
                     /translation="MANDINLDAVLHTFVAEARELLEDMEAALLRVGGEADPRESVNA
                     IFRAAHTIKGSSGLFGLDAIVAFTHVAESVLDEVRDGAVALDEALIALLLACGDYIGR
                     MIDGVEAGATLADPRTDPEGGALLAR"
BASE COUNT          416 a         1018 c          950 g          349 t
ORIGIN      
        1 cccatgagcc cgcggccgcc gcgctgctgc tgaagcgcgc gctcgccttc ctcgcgcgct
       61 gctgagccgc gcggcgctcg cgcgctgctg ccgccgccgc gacaggggta tcgatacgcc
      121 gcgcgggcgt gtagggagtt cttgacggcc ggcgtcggga gatgccaatt ttcgccgtca
      181 tacacctacc ctgtatacgt atcgcgacct catcggccga cattcgattt gcacccgaag
      241 accggccgtg ttcggccctt ccggccgcgc gatcgactcg aataatcgtc gccatcgaag
      301 cgtcctattg agcgagctcg accgctcaag ctcgaacgga agcggtcgta aaccctggcg
      361 atgaacctca tgactccttc ctccagccat ccgtccgcgg ccgagccgtc gctgtcgcgg
      421 cgcctgggcc gcctgcaggc gaccggcctc gcggccgccg ccgccctgtc ggcctggggc
      481 gcgagcctcg cccactcgcc cgcggtcgcg gccgtcgtcg gcgccgtcct cgtcgccgcg
      541 ggcgtcgcgc tcgaccgccg gcgcgcccgg cgcgagcgcc acgagcgcga cgccgccggc
      601 gccttcgtgg ccggcaccga ccagctcggc caggaggtgc tgccgctctg gagcgcgcac
      661 atcgagaact cgcgccagca gatggagacc gcgatcgccg cgctctcgca acgcttcgcc
      721 ggcatcgtcg accgcctcga ccagacgctg cgggcgcaga acgacgacgg cgccggccgc
      781 ggcctggcat cgctgttcga gcacagcgac gcgcggctgc gcgaggtgct cgggtcgctg
      841 aaggaggcga tggcgagcaa cggcgcgatg cacgaggaag tgcagagcct cggccgcttc
      901 gtcgccgagc tgcagcagat ggcggcggag gtcgccgaca tcgcgcagca gaccaacctg
      961 ctcgccatca acgcggcgat cgaggccgcg cacgccggca tcgagggccg cggcttcgcg
     1021 gtgctcgcgc aggaggtgcg caagctgtcg gcgatgtcgg gcgagaccgg ccagcgcatg
     1081 gccgccaagg tcgccgtgat cgcgcaggcg atcgacgcgg cgcgcgcgag cgccgaggcg
     1141 tcggcgcagc gcgaggcggc gtcgctcggc gcgtccgacg acgcgatcaa cgaggtgctc
     1201 ggccgcttcc gcgacgtgac gcaggcgatg gagtcctcgg ccacgacgct caagcacgag
     1261 agcgagggca tccagtccga gatcgtcgag gcgctggtgc agctgcagtt ccaggaccgc
     1321 gtgagccagc gcatgaccca cgtgcgccag aacatggagc gcctgcccgc gctgctcgcg
     1381 gacagccgcc gccgcttcga cgcgagcggc gagctggcct cggtcgacgc gcgcgtgctg
     1441 ctcgacgagc tcgagggcag ctacgcgatg gtcgacgagc gcacgaccca tgccggcgag
     1501 gccggcacgc cggcggcgcc ggccgcgtcc gccagcctcg aggaagtcac cttcttctga
     1561 tccgcgcggc accgcccttc tcccgcatcc cacgacaaca ggaaatccca tggccaagac
     1621 catcatgatc gtcgacgact ccgcctcgct gcgccaggtc gtcggcatcg cgctgcgcag
     1681 cgcgggctac gacgtcctcg aaggctgcga cggcgccgac gcgctcaaga agctgacggg
     1741 ccagaaggtc cacctgatca tcagcgacgt caacatgccc aacatggacg gcatcacgct
     1801 ggtcaaggag gtcaagaagc tcgcgcccta caagttcacg ccgatcgtga tgctcacgac
     1861 cgagtcggcc gacggcaaga aggccgaagg ccaggccgcg ggcgcaaagg cgtgggtgct
     1921 caagccgttc aacccgccgc agctgctggc ggtcgtgcaa aagctcgtgc agccctgagc
     1981 ggccgctcga ccccgtcccc gacgaacgca tccgaacgca ccgccacgcg catgactgcc
     2041 acccccttcc gcatcgacgg cgaattcacg atctaccgcg cggccgaact ggccgagggc
     2101 ctgaaggccg cgctggccgc cacgccggcc ggccatgcgc tggagatcga cctgtccgag
     2161 gtcaccgaga tggactccgc cggcgtccag ctgctgctgg ccgcgcgccg cggcgccgcg
     2221 cagggcgggc gatcgctgcg cctgtccggc cgcagcccgg cggtcgccga ggtcttcgcg
     2281 acgctgcggc tggacgcgca tttcgacgcc gcggccgcca ccgcgcaagc cgagtgaagg
     2341 acgacgacat ggccaacgac atcaacctgg acgcggtgct gcacaccttc gtcgccgagg
     2401 cgcgcgagct cctcgaggac atggaggccg ccctgctgcg cgtgggcggc gaggccgacc
     2461 cgcgcgagag cgtcaacgcg atcttccgcg ccgcgcacac catcaagggc tcgtccggcc
     2521 tgttcgggct cgacgccatc gtcgccttca cgcacgtcgc cgagagcgtg ctcgacgagg
     2581 tgcgcgacgg cgcggtggcg ctcgacgagg cgctgatcgc gctgctgctg gcctgcggcg
     2641 actacatcgg ccgcatgatc gacggcgtcg aggccggcgc gacgctggcc gacccgagga
     2701 ccgacccgga gggcggcgcg ctgctcgccc gcc
//
LOCUS       DASVLN010000509         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_511513, whole genome shotgun sequence.
ACCESSION   DASVLN010000509 DASVLN010000000
VERSION     DASVLN010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511513"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..573)
                     /locus_tag="VF453_12535"
     CDS             complement(1..573)
                     /locus_tag="VF453_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7688531.1"
                     /translation="MHLGGNSMGGGIVLSYAAQHPDEVGSLWLIDAAGIPEAPASELR
                     RMIETTGENPLLVEKESDFPRLVRFTMSQPPWIPGFAMDVMARERMANAELEKKVFVQ
                     IATDSVSDAISGLRVPALITWGAQDRALDVGTAALLHAKLPNSRVDVMPGIGHAPMIE
                     QPERSARDYLRFRAALTPMPPAGAASASAG"
     gene            complement(531..998)
                     /locus_tag="VF453_12540"
     CDS             complement(531..998)
                     /locus_tag="VF453_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7688532.1"
                     /translation="MPLTPRLQRLRRLGRTCALAALAATTLLSAGCASLGDSMTHAAF
                     DAERWHAGLDRREITLPDGTRIAYLDGGSGEPLVLVHGFGADKDNFTRVARWLVPHYR
                     VVAPDLVGFGESTHLPDADYRYAAQAARLATSSARWACSTCTWGATRWAAASC"
     gene            complement(1161..2483)
                     /locus_tag="VF453_12545"
     CDS             complement(1161..2483)
                     /locus_tag="VF453_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7688533.1"
                     /translation="MAELLGALSHALDMTEGQPAGHCLRSCWIGQQLGRAIGLDAAEM
                     RNLYYATLLKDSGCSANAARLSELFLADDRAFKHDRKVAGTNFAGLLRFAVRHTGAQA
                     RLMPRLRALVRAVQHGDDIEAELVETRCTRGAQVARQLRFGDDVADAIHALDEHWDGG
                     GHPQGLAGEEIPLYARIALLAQVVDVYHTGAGPQAAREEVARRRGRWFDPRLADAFAV
                     LARDASFWGQLAADDLEDRVLDLDPAQRRQPVDDDYLDDIAEAFGQIVDAKSPFTAGH
                     SVRVAFVADQVAGELGLDAEVRRWLRRGALLHDVGKLGVPNTLLDKAGPLTAGEWALM
                     QRHAQHTEEILGRISAFGTLARVAGAHHERLDGSGYPRGLDARHIRLETRIITAADIF
                     DALTADRPYRAAMTTAQALATMRAGVGVSVDGDCLAALERALAATGAI"
BASE COUNT          314 a          911 c          933 g          338 t
ORIGIN      
        1 tcagcccgcg ctcgccgacg ccgcgcccgc gggcggcatc ggggtcagcg cggcgcggaa
       61 gcgaaggtag tcgcgcgcgc tgcgctccgg ctgctcgatc atgggcgcat gcccgatgcc
      121 gggcatcacg tcgacacgcg agttcggcag cttcgcgtgc aggagcgccg ccgtcccgac
      181 gtccagcgcg cggtcctgcg cgccccaggt gatcagcgcg ggcacccgca gcccgctgat
      241 cgcgtcgctg accgagtcgg tcgcgatctg cacgaacacc ttcttctcca gttcggcgtt
      301 ggccatgcgc tcgcgcgcca tcacgtccat cgcgaagccg gggatccatg gcggctggct
      361 catcgtgaac cgcaccaggc gcgggaagtc cgattccttc tccaccagca gcgggttctc
      421 gccggtcgtc tcgatcatgc ggcgcagctc gctggccggc gcctccggga tgccggcggc
      481 gtcgatcagc cacaggctgc cgacctcgtc gggatgctgc gccgcgtagc tcagcacgat
      541 gccgccgccc atcgagttgc cccccaggtg cacgtgctgc aggcccagcg cgcggacgaa
      601 gtcgccaggc gcgcggcctg cgcggcgtag cggtagtcgg cgtcgggcag gtgcgtggat
      661 tcgccgaagc cgaccaggtc cggtgcgacg acgcggtagt gcggcaccag ccagcgcgcc
      721 acgcgcgtga agttgtcctt gtcggcgccg aatccgtgca ccagcaccag cggctcgccg
      781 ctgccgccgt cgaggtaggc gatgcgggtg ccgtcgggca gcgtgatctc gcgccggtcc
      841 agtcccgcat gccagcgctc ggcgtcgaac gcggcgtgcg tcatcgagtc gccgagcgac
      901 gcgcagccgg cggacagcag cgtcgtggcg gccagcgcgg cgagcgcgca ggtgcggccg
      961 aggcggcgca ggcgttggag gcggggcgtc aggggcatct ggcttgtgcg gttaggcgcg
     1021 gggacgacgg ctcgactgta cccacgaccc ggccgcgcct cttgaacgaa ggcgacaggc
     1081 cttcgtgcgc gatcctgacg gccacaacat cgaggccgtg tgccacgcgc ccgggtgaga
     1141 gcccgcggag gctccgagcg tcagatcgcc ccggtcgcgg cgagcgcgcg ctccagcgcg
     1201 gccaggcagt cgccgtccac gctgacgccc acgcccgcgc gcatcgtcgc gagcgcctgc
     1261 gccgtcgtca tggccgcgcg gtaggggcgg tcggcagtca gggcatcgaa gatgtcggcc
     1321 gccgtgatga tgcgggtctc cagccggatg tggcgcgcat ccagcccgcg cggatagccg
     1381 ctgccgtcca gccgctcgtg gtgcgcgccg gcgacgcgcg cgagcgtgcc gaacgcgctg
     1441 atgcggccga ggatctcctc ggtgtgctgc gcgtggcgct gcatcagcgc ccactcgccg
     1501 gccgtcagcg gccccgcctt gtccagcagc gtgttgggca cgcccagctt gccgacgtcg
     1561 tgcagcagcg cgccgcggcg cagccagcgc cgcacctcgg cgtcgagccc gagctcgccg
     1621 gcgacctggt cggcgacgaa cgcgacacgc acgctgtggc cggccgtgaa cgggctcttg
     1681 gcatcgacga tctggccgaa ggcctcggcg atgtcgtcca ggtagtcgtc gtcgaccggc
     1741 tggcggcgct gcgccgggtc gaggtcgagg acgcgatcct cgaggtcgtc cgccgcgagc
     1801 tggccccaga acgacgcgtc gcgcgccagc accgcgaagg cgtcggccag gcgcgggtcg
     1861 aaccagcggc cgcggcggcg tgcgacttcc tcgcgcgccg cctgcgggcc ggcgccggtg
     1921 tggtagacgt cgacgacctg cgccagcagc gcgatccgcg cgtacagcgg gatctcctcg
     1981 cccgccaggc cctgcgggtg gccgccgccg tcccagtgct cgtcgagcgc atggatcgcg
     2041 tccgcgacgt cgtcgccgaa gcgcagctgg cgcgcgacct gggcgccgcg cgtgcagcgc
     2101 gtctcgacga gctcggcctc gatgtcgtcg ccgtgctgaa cggcgcgcac gagcgcccgc
     2161 aggcgcggca tcaggcgcgc ctgggcgccg gtgtgccgca ccgcgaagcg cagcaggccg
     2221 gcgaagttcg tcccggcgac cttgcgatcg tgcttgaacg cgcggtcgtc ggcgaggaac
     2281 agctcggaca gccgcgccgc gttggcgctg cagccggagt ccttcagcag cgtcgcgtag
     2341 tagaggttcc gcatctccgc ggcatcgagg ccgatcgcgc gcccgagctg ctggccgatc
     2401 cagcagctgc gcaggcagtg gccggcgggc tgtccctcgg tcatgtccag cgcgtggctc
     2461 aaggctccca gcagttcggc caggcgcggg ccgttg
//
LOCUS       DASVLN010000510         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_511533, whole genome shotgun sequence.
ACCESSION   DASVLN010000510 DASVLN010000000
VERSION     DASVLN010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511533"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VF453_12550"
     CDS             <1..350
                     /locus_tag="VF453_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688534.1"
                     /translation="AAVGRYAAHDPGVGPVLQAQTETLQTWVTVHPKDATAWTLLAQC
                     AEPVGEKLRAIRAEAEAHAANGDLPGAIDRLHAGQKLTRTGKLDFVEASIIDTRLHEL
                     EAQRRELQKEAER"
     gene            complement(373..723)
                     /locus_tag="VF453_12555"
     CDS             complement(373..723)
                     /locus_tag="VF453_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7688535.1"
                     /translation="MKLIVRWLLLAAALLLVAHLSPGVTVRSFGAALGAALVLGLLNM
                     LVRPLLVLLTLPVTVLTLGLFLVVINGLLFWAASGLLDGFRVANFGSALIGSLIYSVA
                     STVIDVAIERLFED"
     gene            1028..1444
                     /locus_tag="VF453_12560"
     CDS             1028..1444
                     /locus_tag="VF453_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEX7688536.1"
                     /translation="MATAKKAAPAKKAPAKKAAAPAKKAAPAAKKAAPAKKAAPAKKA
                     AAPAKKAAPAKKRTPNAAFMKAMTPSPTLAAVIGASPLPRTEVTKKVWEYIKKNGLQD
                     KAKRTMINADAKLKEIFKKAQVSMFEMTKLINSHLS"
     gene            1702..>2084
                     /locus_tag="VF453_12565"
     CDS             1702..>2084
                     /locus_tag="VF453_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688537.1"
                     /translation="MSIERSTELLREALPWMSRQAAGLHPVSYAVWFEYVARTNPPLR
                     AAIDEHLAREGRLDEFATHALFKRHVADVDPESAQRVVDGMQRVLGGIGEVAASAGAH
                     AARFGASLAQLAGALERGDAVRGIG"
BASE COUNT          388 a          717 c          722 g          257 t
ORIGIN      
        1 aggcggcggt cggccgctac gcggcgcacg acccgggcgt cggccccgtc ctgcaggcgc
       61 agaccgagac gctgcagacc tgggtgaccg tgcatccgaa ggacgcgacc gcgtggacgc
      121 tgctcgcgca gtgcgccgag cccgtggggg agaagctgcg cgccatccgc gccgaggccg
      181 aggcgcatgc cgccaacggc gacctgcccg gcgcgatcga ccggctgcac gccggccaga
      241 agctgacgcg cacgggcaag ctggacttcg tcgaggcgtc catcatcgac acccgcctgc
      301 acgagctcga ggcgcagcga cgcgagctgc agaaggaagc cgagcgctga gcgacccggc
      361 gcgcggcccg cttcagtctt cgaacaggcg ctcgatcgcg acgtcgatca ccgtcgacgc
      421 gacgctgtag atcagcgagc cgatcagcgc cgagccgaag ttggcgacgc gaaacccgtc
      481 gagcagcccg gacgcggccc agaacagcag cccgttgatc acgacgagga acagcccgag
      541 cgtcagcacc gtcaccggca gcgtgagcag cacgagcagc gggcgcacca gcatgttcag
      601 caggcccagc accagcgccg cacccagcgc cgcgccgaag ctgcgcacgg tcactcccgg
      661 cgacaggtgc gccaccagca gcagcgcggc ggcgagcagg agccagcgga cgatgagttt
      721 catggcgtgg catgcgcggt tggacgcgcc cattgtgggg caagcgccgc gcggcctgcg
      781 ccgcggtcgc gcgcggcctc tgctgaaaat acggggtggg gtatgacaat ccccacggct
      841 gatgcctccg agcgacaaaa agatgcgttt ccctcgtgag aaaggcgatt ttcataggta
      901 agaccgcttc tcacgccctc gtgcaggcag taccattcgt tcgctgcgga tccatggaga
      961 aacgagagtt catgcgacgc tcacccaggc ccggcacatc aatgacctct tgatggagag
     1021 aatcgacatg gcaactgcaa agaaggccgc tccggccaag aaggccccgg caaagaaggc
     1081 cgcggctccg gcgaagaagg ccgctccggc ggcgaagaag gccgcgcccg cgaagaaggc
     1141 ggcgccggcc aagaaggccg cggctccggc gaagaaggcg gctccggcca agaagcgcac
     1201 gccgaacgcc gcgttcatga aggcgatgac gccttcgccg accctggccg ccgtgatcgg
     1261 cgcgtcgccg ctgccccgca ccgaagtcac caagaaggtg tgggagtaca tcaagaagaa
     1321 cggcctgcag gacaaggcca agcgcacgat gatcaacgcc gacgccaagc tgaaggaaat
     1381 cttcaagaag gcgcaggtct cgatgttcga gatgaccaag ctgatcaaca gccacctcag
     1441 ctgatcctgc agcgctgagc ctgaacggcc gacgatggcg aaagcctcgt cggccttctt
     1501 gtttgtgcgg cccgttgccg gcgtcgccgc tcgcccgcct cctcgtcgct gcgcggccgc
     1561 attccgtgcg ataagcccgc cgccgccgcc gaccattcgg acgctccacg gcggtgcgca
     1621 ggcagaatgc gcggcaggag agcgtcccgc ctggcgagga tggccctata aaccgtcgct
     1681 gaggatcgat tgagatacga gatgagcatc gagcgcagca ccgagctgct gcgcgaggcg
     1741 ttgccctgga tgtcgcgcca ggccgcgggg ctgcatcccg tgagctacgc cgtctggttc
     1801 gagtacgtcg cgcgcaccaa cccgccgttg cgggccgcga tcgacgagca ccttgcgcgg
     1861 gagggccggc tcgacgagtt cgccacccac gcgctgttca agcgccatgt cgccgacgtc
     1921 gacccggagt cggcgcagcg cgtcgtcgac ggcatgcagc gggtgctcgg cggcatcggc
     1981 gaagtcgcgg catcggccgg cgcgcacgcc gcgcggttcg gcgcctcgct cgcgcaactg
     2041 gccggcgcgc tggagcgcgg cgacgcggtg cgcgggatcg gcca
//
LOCUS       DASVLN010000511         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_348280, whole genome shotgun sequence.
ACCESSION   DASVLN010000511 DASVLN010000000
VERSION     DASVLN010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348280"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..791
                     /locus_tag="VF453_12570"
     CDS             153..791
                     /locus_tag="VF453_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688538.1"
                     /translation="MTAGASRRAVLGRAGALAIGIAGGLAGCGMLGRRVTPAGPGVPG
                     IYPAPVEDFGRWDRFPVEVAGYRRGAVVRYAPGLVDYSIAYERMDAELQDAVTLYFYP
                     RMNDAAAQVAQEEAQVLQSHPGARVLDRRDLPLQRNGATYTARLATFEFEEVFAGRLQ
                     RVSSQLLVAFRALGTFKARSTAPFAQAVQAQASFLRLLEGVAWDAPGEIGAQ"
     gene            829..>1578
                     /locus_tag="VF453_12575"
     CDS             829..>1578
                     /locus_tag="VF453_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7688539.1"
                     /translation="MERFFRIDHDAATGVAELVLDQPARMNTMTPAFFLALRDAVRTL
                     DDAGATRVLVVRAEGKHFSAGMALEVFGGDSPLLDTSTARRRLSFQAQLRRLMACFDA
                     LDEARFPVVCAVQGACVGGALDLAAACDIRICAADAFFTVQEIHVGMAADLGVLQRLQ
                     RQLPQGLARELAYTGDRLAADRALAVGFVNAVLPDADALLAHARKLATAIAAKSPVAV
                     AASKIALNHARDHATADALERMALLQSAVFDA"
BASE COUNT          180 a          587 c          587 g          224 t
ORIGIN      
        1 atgtggctga cggcctacgt cggcaacgcg cgtgcgctcg cgttctaccc gcgccagggc
       61 tacgccgacg cgggcgcgac ggtcttcgag ttcgagggcg agtcgcacgg caaccggatc
      121 ttcgtgaagc cgctggccgc ggaggcgtcg cgttgacggc cggcgcgtcc cgccgcgcgg
      181 tgctcggacg cgccggggcg ctcgcgatcg ggatcgcggg cggcctggcc ggctgcggca
      241 tgctgggccg gcgcgtgacg cccgcggggc cgggcgtgcc gggcatctac cccgcgccgg
      301 tcgaggactt cgggcgctgg gatcgcttcc ccgtcgaggt cgcgggctac cgccgcggcg
      361 cggtcgtgcg ctatgcgccg gggctggtcg actactcgat cgcctacgag cgcatggacg
      421 cggagctgca ggacgcggtc acgctgtact tctatccccg catgaacgac gccgccgccc
      481 aggtcgcgca ggaggaggcc caggtgctgc agagccatcc cggcgcgcgg gtgctcgatc
      541 ggcgcgacct gccgctgcag cgcaacggcg ccacctacac ggcgcggctc gccacgttcg
      601 agttcgagga ggtgttcgcc ggccgcctgc agcgcgtgtc gtcgcagctg ctggtcgcgt
      661 tccgcgcgct cggcaccttc aaggcgcgca gcaccgcgcc cttcgcccag gccgtgcagg
      721 cgcaggcgtc gttcctgcgg ctcctggagg gcgtcgcctg ggacgcgccg ggcgagatcg
      781 gcgcgcagta ggctcgcggc caccgctttc ttcgacgagg atctccgcat ggaacgcttc
      841 ttccgcatcg accatgacgc cgcgaccggc gtcgccgagc tcgtgctcga ccagcccgcg
      901 cgcatgaaca cgatgacgcc ggccttcttc ctcgcgctgc gcgacgccgt gcggacgctc
      961 gacgacgccg gcgcgacgcg cgtgctggtc gtgcgcgccg agggcaagca cttctcggcc
     1021 ggcatggcgc tggaagtgtt cggcggcgac tcgccgctgc tggacacgtc caccgcgcgc
     1081 cgccggctct cgttccaggc gcagctgcgg cggctgatgg cctgcttcga cgccctggac
     1141 gaggcgcgct tcccggtcgt ctgcgcggtg cagggcgcgt gcgtgggcgg cgcgctcgac
     1201 ctcgcggcgg cgtgcgacat ccgcatctgc gccgccgacg cgttcttcac ggtgcaggag
     1261 atccacgtcg gcatggccgc cgacctgggc gtcctgcaac gcctgcagcg ccagctgccg
     1321 cagggcctcg cgcgcgagct cgcgtacacg ggcgaccgcc tcgccgccga ccgggcgctc
     1381 gcggtcggct tcgtcaacgc cgtgttgccg gacgcggacg cgctgctcgc gcacgcgcgc
     1441 aagctggcga cggcgatcgc ggcgaagtcg ccggtggccg tcgcggccag caagatcgcg
     1501 ctgaaccacg cgcgcgatca cgcgaccgcc gacgcgctcg agcgcatggc gctgctgcag
     1561 agcgccgtct tcgacgcc
//
LOCUS       DASVLN010000512         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_295953, whole genome shotgun sequence.
ACCESSION   DASVLN010000512 DASVLN010000000
VERSION     DASVLN010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295953"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..2354)
                     /locus_tag="VF453_12580"
     CDS             complement(138..2354)
                     /locus_tag="VF453_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7688540.1"
                     /translation="MHRPAVGAALVSAVLASLGASAQAQEAAAAAARPAAAASQSTQA
                     DTQTVTVTATRRREPARDVPVQVNALSAENLERAGATSLSDYVGGLPGVDVKTSNGPG
                     LGQVSIRGVTTGDQTIATVGVYVDDVAFGSSTAFAAGSTMALDMSLLDLNHIEVLRGP
                     QGTLYGAGSMGGLLKYVTNEPDTYELSGKVSLGLNGIKGGGVGHVESGVVNVPLKEGL
                     AAVRVALFSDHQGGYIDAVGPAAGRNINRGDTTGGRVSLLVEPTSRLHLRLTAVDQNI
                     ARRGNDIVDYDIATGRPTEGDLVRQLGIGEPYHVNIGLVSADVEYDMGWARVNSITSV
                     QQEKTRNRQDTGFYDAALTGAVGEPVTDSRLDGEPGARKHTQEFRLTSAPGTLEWLGG
                     LYFDSEVGRNSQVLAGTIVSSQTSVDLITDQQPSTYKELAGYGDVTWNMAKDWSLTGG
                     LRVARNRQIYGVVTNGVAEGGNSAETARTYLATVRYAIDPVSNVYFRAASGYRPGGPN
                     PPAFDAGGNPIPGTPASFKSDSLWSYELGYKGDLLDKRLSIETSIYDIEWKDIQQPVA
                     VGSGTLIENAGRARVRGAELFTRYRATPALSLDASLSAIDAKLTENAPAMGPAGSRLP
                     NSARFSGTLGFTDSFAVARRSAYAGASVRYVGERNAGFDGSESSQPNFRMPGYTLVDA
                     QGGVTLGKVDLGLYVRNLFDKRAILGADAALVAFGSPLHATVAQPRTVGATLSVAF"
     gene            2819..3787
                     /locus_tag="VF453_12585"
     CDS             2819..3787
                     /locus_tag="VF453_12585"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX7688541.1"
                     /translation="MSAANLLVWDNHACLPLRPHDLAFLPQLERHRAAGTHVVMVNVG
                     YGDDGIEQHVRMLATLRAWIAAHADRFRLIQSVDDVERARAAGQLAVGFDIEGANAVD
                     DQLDLVGVYRDLGVRWMLLAYNRNNRAGGGCQDEDGGLTPFGRALLEELAARGVVACC
                     SHTGYRTAREAIDASPTPVIFSHSNPRALADHPRNIPDDLIRACAARGGVVGINGIGL
                     FLGDNDASPARVARAAAYVADLVGAQHVGIGLDFLHDRSELDATIAAHPEQFPPELGY
                     GSGMAFMAPEQIPEVADHLRALGMAEPEVAGILGGNWLRVARQVWK"
     gene            3960..>4524
                     /locus_tag="VF453_12590"
     CDS             3960..>4524
                     /locus_tag="VF453_12590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7688542.1"
                     /translation="MTHDFIVIGAGIAGASLAAHLAQRGRVLLLEGESQPGYHTTGRS
                     AALFSIIYGNAPVRALSRASRDFLFQPPPGFTDVPLVHARPTLFFARQEQLALIDEAR
                     IDDDVRRSTRVLDAAGVSAMLPVFRPGYAAGGMVDDASADIDVHALHMGYLRQARRLG
                     ATLLTDRPVRSLARDADGWRVHAAGEDY"
BASE COUNT          616 a         1654 c         1595 g          659 t
ORIGIN      
        1 cctcgcggcg cgcgacgcct gggaacgact cggccgcccc gaccgcagcg agctgtgagc
       61 ccacgcctcg cggacacgtc cggcgggcgc ggccaaacgc atgcgcccgc cccgggggcg
      121 cgcgcgccga cgcgctctca gaacgccacc gacagcgtgg ccccgaccgt gcgcggctgc
      181 gccaccgtcg catgcagcgg gctgccgaac gcgacgagcg ccgcatcggc gccgaggatg
      241 gcgcgcttgt cgaacagatt gcgcacgtac aggccgaggt cgaccttgcc cagcgtgacg
      301 ccgccctgcg cgtcgaccag cgtgtagccg ggcatcctga agttgggctg cgaggactcg
      361 ctgccgtcga agccggcgtt gcgctcgccg acgtagcgca cgctggcgcc ggcgtaggcg
      421 gagcgccgcg cgacggcgaa gctgtcggtg aagcccagcg tacccgagaa gcgcgccgag
      481 ttcggcagcc gcgagcccgc agggcccatc gccggcgcgt tctcggtcag cttcgcgtcg
      541 atggccgaca ggctcgcgtc caggctcagc gccggcgtgg cgcggtaacg cgtgaacagc
      601 tcggcgccgc gcacccgcgc gcgaccggcg ttctcgatca gcgtgcccga acccaccgcg
      661 accggctgct ggatgtcctt ccactcgatg tcgtagatcg acgtctcgat ggacaggcgc
      721 ttgtccagca ggtcgccctt gtagccgagc tcgtagctcc agagcgagtc cgacttgaag
      781 ctggccggcg tgccggggat cggattgccg cctgcgtcga aggccggcgg gttcgggccg
      841 ccgggccggt agccgctggc ggcgcggaag tagacgttgc tgaccgggtc gatcgcgtag
      901 cgcaccgtgg ccaggtaggt gcgggccgtc tccgccgaat ttccgccctc ggccacgccg
      961 ttggtcacga cgccatagat ctggcggttg cgcgcgacgc gcagtccgcc cgtgagcgac
     1021 cagtccttgg ccatgttcca ggtgacgtcg ccgtagcccg ccagttcctt gtatgtcgag
     1081 ggttgctggt ccgtgatcag gtcgaccgag gtctgcgacg acacgatcgt tcccgcgagg
     1141 acctgcgagt tgcggccgac ctcgctgtcg aagtaaaggc cgccgagcca ttcgagcgtg
     1201 ccgggcgccg aggtcaggcg gaactcctgc gtgtgcttgc gcgcgcccgg ctcgccgtcc
     1261 aggcgcgagt cggtgaccgg ttcgccgacg gctcccgtca gcgcggcgtc gtagaacccg
     1321 gtgtcctggc ggttgcgcgt cttctcctgc tgcaccgacg tgatcgagtt gacgcgcgcc
     1381 cagcccatgt cgtactcgac gtcggcggag acgaggccga tgttgacgtg gtacggctcg
     1441 ccgatgccca gctggcgcac gaggtcgccc tcggtggggc ggccggtggc gatgtcgtag
     1501 tcgacgatgt cgttgccgcg gcgggcgatg ttctggtcga ccgcggtcag gcgcaggtgc
     1561 agccgcgacg tcggctcgac cagcagcgac acccgcccgc cggtcgtgtc gccgcggttg
     1621 atgttgcgcc cggcggccgg gccgacggca tcgatgtagc cgccctggtg gtccgagaag
     1681 agcgcgaccc gcacggcggc caggccctcc ttcagcggca cgttgaccac gccgctctcg
     1741 acgtggccga cgccgccgcc cttgatgccg ttcagtccca gcgagacctt gccggacagc
     1801 tcataggtgt cgggttcgtt ggtgacgtac ttcagcaggc cgcccatcga gccggcgccg
     1861 tacagcgtgc cctgcgggcc gcgcagcacc tcgatgtggt tcaggtcgag cagcgacatg
     1921 tccagcgcca tcgtggagcc ggcggcgaag gcggtgctcg agccgaacgc gacgtcgtcg
     1981 acatagacgc cgacggtggc gatggtctgg tctcccgtgg tcacgccgcg gatgctcacc
     2041 tggccgagtc ccggcccgtt ggaggtcttc acgtcgacgc ccggcagtcc gccgacgtag
     2101 tcgctcagcg acgtcgcgcc ggcgcgctcg aggttctcgg ccgacagggc gttgacctgc
     2161 accgggacgt cgcgcgcggg ctcccggcga cgggtcgccg tgacggtgac cgtctgggtg
     2221 tcggcctgcg tggactgcga cgcggcggcg gccggcctcg cggcggcggc cgccgcttcc
     2281 tgcgcctgcg cggacgcgcc gagcgaggcc agcaccgccg agacgagggc ggcgccgacg
     2341 gcgggccgat gcacgggctt tgcgtgttgg aacgaagtgc gggggtgagg gatcgtcatg
     2401 cttcggctcc atcgaatttt ccgaagtaga aaccacaaca ccttgctaga caagtatttt
     2461 cgaatcagtg taaacgatga tcgacgacgc ggagtcgggg gacttcggcg gcggcgacac
     2521 tccgcccacg agcgaggagg acgccgagga gggcctcctc cgagcgccgt gcggcaagac
     2581 cgcgaggcgt cggcggttcg cggcgggtcg gcgacttccg tgttcaatgt tccattttgg
     2641 aaaattttta tttatagtgg acatcatcga tacggaaatc gcacaggaga gcgcatgctc
     2701 caccgccgcc aacatgtccg cgggccgagg ctccgcaccg ccgagggccg ccatccatga
     2761 acccccgacc ggccgttccg aagggggctc gcaccgcagt ccgcaggacg gtggcctcat
     2821 gagcgccgcg aacctcctcg tctgggacaa ccacgcgtgc ctgccgctgc ggccccacga
     2881 cctcgccttc ctgccgcagc tggaacgcca ccgcgccgcc ggcacgcacg tcgtgatggt
     2941 caacgtgggc tacggcgacg acggcatcga gcaacacgtg cgcatgctcg cgacgctgcg
     3001 cgcgtggatc gccgcgcacg ccgaccgctt ccgcctcatc cagtcagtcg acgatgtcga
     3061 gcgcgcccgc gcggcgggcc agctggcggt cgggttcgac atcgaggggg ccaacgccgt
     3121 cgacgaccag ctcgacctcg tcggcgtcta ccgcgacctc ggcgtgcgct ggatgctgct
     3181 cgcctacaac cgcaacaacc gcgccggcgg cggctgccag gacgaggacg gcggcctcac
     3241 gccgttcggt cgcgcgctgc tcgaggagct ggcggcccgc ggcgtggtcg cctgctgctc
     3301 gcatacgggg taccgtaccg cgcgggaagc gatcgacgcc tcgccgacgc cggtgatctt
     3361 ctcgcattcg aacccgcgcg cgctggccga ccatccgcgc aacatcccgg acgacctgat
     3421 ccgcgcctgc gcggcgcgcg gcggcgtcgt cggcatcaac ggcatcggcc tgttcctggg
     3481 cgacaacgac gcctcgcccg cgcgcgtggc acgcgcggca gcctatgtcg ccgatctcgt
     3541 cggcgcgcag cacgtaggca tcggcctcga cttcctgcac gaccgcagcg aactcgacgc
     3601 gaccatcgcc gcccatccgg agcagttccc gccggagctc ggctacggca gcggcatggc
     3661 cttcatggcg cccgagcaga tccccgaggt cgccgaccac ctgcgcgcgc tgggcatggc
     3721 cgagcccgag gtcgccggca tcctcggagg caactggctg cgcgtcgcgc gccaggtctg
     3781 gaaatgacgg cccggctcgc ttcgctcccc gcccccaagg ggctcacgcc cgctcgctcc
     3841 ggagcggccg agcgctcgcc ggcggacggc atgacgaccc ggctcgcttc gctccccgcc
     3901 cccaaggggc ccacgccggc cagcacctgg gcgcgtcgcg cgcgcccctc ccccacccca
     3961 tgacccacga cttcatcgtc atcggcgccg gcatcgcggg cgcatccctc gcggcccacc
     4021 tggcgcagcg cggccgcgtg ctcctgctcg agggcgagag ccagcccggc taccacacga
     4081 cgggccgctc ggcggcgctg ttctcgatca tctacggcaa cgcgccggtg cgtgcgctca
     4141 gccgcgcgag ccgcgatttc ctgttccagc cgccgcccgg gttcaccgac gtgccgctgg
     4201 tgcacgcgcg cccgacgctg ttcttcgccc gccaggagca gctcgccctg atcgacgagg
     4261 cgcgcatcga cgacgacgtg cgccgttcga cgcgcgtgct ggacgccgcc ggcgtgtccg
     4321 cgatgctgcc cgtctttcgc cccggctatg cggcgggcgg catggtcgac gacgcgtccg
     4381 cggacatcga cgtgcacgcg ctgcacatgg gctacctgcg ccaggcgcgg cgcctgggcg
     4441 cgacgctgct gacggatcgc cccgtgcgct cgctcgcgcg cgacgcggac ggctggcgcg
     4501 tgcacgcggc cggcgaggac tacc
//
LOCUS       DASVLN010000513         2789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_240968, whole genome shotgun sequence.
ACCESSION   DASVLN010000513 DASVLN010000000
VERSION     DASVLN010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240968"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(633..1541)
                     /locus_tag="VF453_12595"
     CDS             complement(633..1541)
                     /locus_tag="VF453_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7688543.1"
                     /translation="MPDRLVPSQLVATGPLDIACHLAGPQGGPAVMLLHGFPYDAQAY
                     AEVAPRLAAQGCRVVVPWLRGHGQTRFRDSATPRSGQQAAIGDDARALMEALGIERAV
                     LAGYDWGGRAACVVAALWPERCAGLVSGNGYLIQDIAHAAVPIAPEREAALWYQYYFH
                     GERGRAGLEANRRALARLLWTTWSPTWHFDGAEFERSAPAFDNPDWVDVVLHSYRHRH
                     GLAPGDSRYDALERALAALPPIAVPTITLDGTDDTVTPPRDGRDDARHFTGWREHRLV
                     PGAGHALPQEAPTAFAEAVMALVRRA"
     gene            complement(1696..1977)
                     /locus_tag="VF453_12600"
     CDS             complement(1696..1977)
                     /locus_tag="VF453_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688544.1"
                     /translation="MRPSLIAIVVSVAELLAGAIPFALFAWINAMFAVLNLFTYKPTP
                     YVQTTYMAVYLAAAFAEIAVFAKWRTGSVVVPAVPLVYGMVAGVFSRAG"
     gene            complement(2049..>2789)
                     /locus_tag="VF453_12605"
     CDS             complement(2049..>2789)
                     /locus_tag="VF453_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688545.1"
                     /translation="DAANAAREARRLAGDRVGRFGFLGGSQGGWVAPLAALESGADFL
                     EVGFGVVGTAWEQDQWQVDYQLVHEHGFDPAILPRVHELTDATAAVARWDFRAGMARV
                     DALKAEFAREPWLAHVDGQYSGGLLAGEVDRMRAESPQVPWNYAGLDVIRSLRVPQLW
                     VFAADDDVAPSAPSIERLGSIREDGVPLRRIVFPDTTHGILRIARAADGRRRDLGIFA
                     PGYFPLLIDFAKGTPRAAYGDARWLERY"
BASE COUNT          410 a         1010 c         1015 g          354 t
ORIGIN      
        1 ccccacccgc cgcgaacgac acggcgccgc tcgcgcgctc gctgttcgac gagccgcacg
       61 acgacgcgac acccgcctcc tgagcgtcgt cacgcgacgt tgcgcggcgg cctcgctcgc
      121 cgggcggacc gcaaggacgg cgcgggcgtt gccgacgaga gcgtcggcgc tctcggacgg
      181 gagtcgccct gtcgcgaagg cgctgattgc ggtttgcaac gccggccttc ccttccaaaa
      241 catcggcgtc gatgtcttca atcggaagct tggtgttcgc aaccgcaaat cggcgcgtcg
      301 ccagcgagcg aaatgccgtc ccgaacgcct gtcttcgact ccagaacatc ggcgccgatg
      361 ttttcaaacg gaagcccggt gtttgcaacc gcaaatcgcc gcgtcgccaa cgagcgaatt
      421 gccgtctcga acccccgtct tcgattccag aacatcggtg ccgatgtttt caaacggaaa
      481 ccgggtgttt tcggcgccga cgtttcgacc ggaacctcgt tggctgcaag cgcaaaccgc
      541 cgcgtcgcac aagcgcgggt tggttgtcga gcctgtcccg ccgggcgcca gaccgccgtg
      601 atgtcgctgg cccggaacgc cgatgtgccg cctcacgcgc gccgcacgag cgccatcacc
      661 gcctccgcga acgccgtggg cgcttcctgc ggcaacgcat gcccggcgcc cggcacgagc
      721 cggtgctcgc gccacccggt gaagtgccgg gcgtcgtccc ggccgtcgcg cggcggcgtg
      781 acggtgtcgt cggtgccgtc cagcgtgatc gtcggcacgg cgatgggcgg tagcgcggcc
      841 agcgcacgct cgagcgcgtc gtagcgagag tcgcccggcg ccaacccgtg ccggtgccgg
      901 tagctgtgga ggacgacatc gacccagtcc ggattgtcga acgccggcgc gctgcgctcg
      961 aactcggcgc cgtcgaagtg ccaggtcggg gaccaggtcg tccagagcag gcgtgccagc
     1021 gcgcgccggt tcgcctcgag cccggcgcgt ccgcgttcgc cgtggaagta gtactggtac
     1081 cagagcgcgg cctcgcgctc gggcgcgatc ggcaccgccg cgtgcgcgat gtcctggatc
     1141 aggtagccgt tgcccgagac cagcccggcg cagcgctccg gccacagcgc cgcgacgacg
     1201 caggccgccc ggccgcccca gtcgtagccg gcgagcacgg cccgctcgat gccgagcgcc
     1261 tccatcagcg cccgcgcgtc gtcgccgatg gccgcctgct ggcccgagcg cggcgtcgcg
     1321 gagtcgcgga accgcgtctg cccatggccg cgcagccacg gaacgacgac cctgcacccc
     1381 tgcgcggcca gccgcggcgc gacctcggca taggcctggg cgtcgtaggg gaagccgtgc
     1441 agcagcatga ccgcgggccc gccctgcggc ccggccaggt ggcaggcgat gtcgagcggt
     1501 ccggtcgcga cgagctgcga gggaacgagg cggtcgggca tgcgggctcc agcgaatgtc
     1561 gcgacagcat acgcggcccg cgcatgccgg ccgccacggg gcaatggcgc gacggcacgg
     1621 aagcgacggt gctgccgcgc gcgggacggg ctggggcgcg acaccggcgc cgggcatcgc
     1681 ccggcaggag ccggctcatc ccgcgcggct gaacacgccg gcgaccatcc cgtagaccaa
     1741 gggcacggcc ggcaccacga ccgagccggt gcgccacttc gcgaagaccg cgatctcggc
     1801 gaacgccgcc gcgaggtaga cggccatgta ggtcgtttgc acgtacggcg tcggcttgta
     1861 cgtgaacagg ttgagcaccg cgaacatcgc gttgatccag gcgaacaacg cgaacgggat
     1921 ggcgccggcc aggagctcgg cgacggacac gacgatggcg atcaggctcg ggcgcatggt
     1981 tcggtgggcc gggcggacgc aggcactgta catgcggcgc gcggatggcc gcccaggagc
     2041 gccggcagtc agtaccgttc gagccaccgt gcgtcgccgt aggccgcgcg cggggtgccc
     2101 ttcgcgaagt cgatcagcag cgggaagtag ccgggcgcga agatgccgag gtcgcggcgg
     2161 cggccgtcgg cagcgcgcgc gatccgcagg atgccgtggg tggtgtccgg gaagacgatc
     2221 ctgcgcagcg gcacgccgtc ctcgcggatc gagccgaggc gctcgatcga cggcgcgctc
     2281 ggggcgacgt cgtcgtcggc cgcgaacacc cacagctgcg gcacgcgcag gctgcggatc
     2341 acgtcgagcc cggcatagtt ccagggcacc tgcgggctct cggctcgcat gcggtcgacc
     2401 tcgccggcca gcagtccgcc cgagtactgg ccgtcgacat gcgcgagcca gggctcgcgg
     2461 gcgaattcgg ccttgagcgc gtcgacgcgc gccatgccgg cgcgaaagtc ccagcgcgcg
     2521 accgccgccg tcgcgtcggt cagctcgtgg acccggggca ggatcgccgg gtcgaagccg
     2581 tgctcgtgca cgagctggta gtcgacctgc cactgatcct gctcccacgc ggtgcccacg
     2641 acgccgaagc cgacctcgag aaagtcggcc cccgattcca gcgcggcgag cggcgcgacc
     2701 cagccgccct ggctgccgcc caggaagccg aagcggccga cgcggtcgcc cgccaggcgg
     2761 cgcgcctcgc gggcggcgtt ggcggcgtc
//
LOCUS       DASVLN010000514         6240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_511859, whole genome shotgun sequence.
ACCESSION   DASVLN010000514 DASVLN010000000
VERSION     DASVLN010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511859"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VF453_12610"
     CDS             complement(<1..531)
                     /locus_tag="VF453_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEX7688546.1"
                     /translation="MRHSITARLVAMFALATLATFALIGAALYGVLERELTRHEDDEL
                     NTYLQTMRYSIERFGDLEHWPRLQVKMDTLTPADGSVRFWVLSDDARFQYGKGLDEME
                     RATPSVDGHGTLVLPVSGRTFRTLSARLGPLAERPAVKLIVGVDTAPFARTMRRCLVA
                     LALLSLGAGGLVAGLGW"
     gene            complement(528..1223)
                     /locus_tag="VF453_12615"
     CDS             complement(528..1223)
                     /locus_tag="VF453_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HEX7688547.1"
                     /translation="MKLLIVEDELKTAEYLRKGLTEQGCAVDVAHDGVEGQHLALVHD
                     YDIVVLDAMLPGIDGFTLLRALRAVKQTPVIMLTARDSIEDRVRGLHDGADDYLVKPF
                     SFLELLARLQALQRRGRSQEPAQLRIANLQIDLIARKAWRGGERIDLSAKEFALLAVL
                     ARRQGEILSKTAIAELVWDMNFDSNTNVVEVAIKRLRAKIDAAFAPKLLHTIRGMGYV
                     MEVRDEPTDGEAA"
     gene            complement(1258..4482)
                     /locus_tag="VF453_12620"
     CDS             complement(1258..4482)
                     /locus_tag="VF453_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7688548.1"
                     /translation="MSVSEGFIKRPIGTTLLALAILLVGIAVYPLLPVAPLPQVDFPT
                     IQVSAKLPGASPETMASNVAQPLERQFSLIAGLTQMTSTNAIGSTQITLQFDLNRAID
                     GAALDVQAAINAATGQLPTNLPSPPTFRKVNPADSPIMIMAVHSDTLPLIQTNDYADN
                     ILAQQISQITGVGLVNIGGAQKPAVRVQVDPAKLASMGMSLEDVRGVIATTSVNAPKG
                     TIDGPSQSFTVYTNDQLLKAAPWNDVVLAYRNGAPVRVRDIGVAVDAPEDSKVHAWAF
                     SGAAAPDSVGNGRAMLLIIYKQPGANVIETVDKVKEALPRLRAAIPPTVHVDTLIDRT
                     QTIRASVHDVEFTLLLTIALVVMVIFIFLRNVAATLIPSVTVPLALLGTAAIMYVVGY
                     SLDNLSLMALTIAVGFVVDDAIVMLENIYRHVEEGMPPMEAAIKGAGEIGFTIISISV
                     SLVAVFIPLLLMGGIVGRLFREFAVTVTLTIGVSVIVSLTLTPMLCSRFLKDEHAKRH
                     GRLYQLFEAGFDRMLGAYRRGLDVVMRHQFITLLSFLATVTATVLLFIAIPKGFFPQQ
                     DTGFIFGNAQSSQDSSFESMNRRQIQFADIIRQDPDVTGVGMFGNSSQFNTGNFFIVL
                     KPKDQGRRLSADQIIARLRPKLAKVGGASLLMQAGQDINVGGRLARTQYQYTLTDANI
                     DELNEWAPKLLERLQKLPQLTDVASDMQNAAATASLTIDRDRAASFGISPSLIDATIY
                     DAIGQRQVNQYFTQINSYHVVLEVTPKLQQDPSLFDKLYLTSPITGRQVPLSSFVKVD
                     TDKTAYLAISHQGQFPAVTISFNLAPGASLGKAVEAINQAQAQMGLPQALNGQFQGTA
                     QAFRDSLRTQPYLIAAALVAVYIVLGLLYESFIHPVTILSTLPSAGVGALLILMAGGY
                     DLSVIALIGIILLIGIVKKNGIMMVDFALTAERDQGLTPQEAIYRACLLRFRPIMMTT
                     MCALLSGLPLMLGHGAGSELRRPLGFAMVGGLVLSQVLTLFTTPVIYLYLDRAHYWYM
                     KRKEARAKRKAKMGKDGGPGTGGFAPDGTDPDRRLPVPTGGEPR"
     gene            complement(4479..5717)
                     /locus_tag="VF453_12625"
     CDS             complement(4479..5717)
                     /locus_tag="VF453_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7688549.1"
                     /translation="MKTLPFDSRARWAIASAATLVVAAAGLWALSHDSAQAADKDAKR
                     KADDAPVQVVTAPVKAQDMPILRTGLGTVAATYSVTVKARVDGQLDAVGFAEGQDVKK
                     GQVIARIDPRTFQATLMQAQAQRAKDEATLANARVDLQRYTTLAAQDAATQQQLDTQK
                     ALVKQLEATVKSDDAQVNSAQVQLGYTTIAAPISGRAGARLVDPGNIVHAADASGLVV
                     INQIDPITVAFTLPEDAVQATNRAMAASHAPLVVQAWSRDGSELLGTGTLTLVNNQID
                     TTSGTVQMKGSFPNPQHALWPGQFVNVRLVLGTRRQAITIPAAAVQRSESGVFTYVVD
                     PGGSARAQTIGLVQIQDGVAIVDQGLQPGQRVVVDGQYKLKPGSRTVEAPARGASAPA
                     GAASDGKRDTSGQGDSGSGK"
     gene            complement(5714..>6240)
                     /locus_tag="VF453_12630"
     CDS             complement(5714..>6240)
                     /locus_tag="VF453_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7688550.1"
                     /translation="GGAAAQMSQLFDTPARVWSLGAALAQALFDGGLRRARTAQAEAA
                     YDAAVAQYKQTVLSGFQQVEDQLATLRELARESGYQDEAVRAAQTSERLALLQYRAGT
                     NSYLNVVTAQQLSLGAQRAAVQLRGRQLAASVSLIAATGGGWRADRPDAVPSATVPAA
                     STAAQARTIQEPTT"
BASE COUNT          884 a         2137 c         2235 g          984 t
ORIGIN      
        1 ccagcccagc cccgcgacca gcccgcccgc gccgagcgac agcaacgcca gcgccacgag
       61 gcagcgccgc atcgtgcgcg cgaacggcgc ggtgtcgacg ccgacgatca gcttgaccgc
      121 gggccgctcg gccagcggtc cgaggcgcgc cgacagcgtg cggaacgtgc gcccgctgac
      181 cggcagcacc agcgtgccgt ggccgtcgac ggacggcgtc gcgcgctcca tctcgtccag
      241 ccccttgccg tactggaaac gcgcatcgtc gctcagcacc cagaagcgca cgctgccgtc
      301 ggccggcgtg agcgtgtcca tcttgacctg cagccgcggc cagtgctcga ggtcgccgaa
      361 gcgctcgatc gagtagcgca tcgtctgcag gtaggtgttg agttcgtcgt cctcgtggcg
      421 cgtgagctcg cgctcgagca cgccgtacag cgccgcgccg atcagcgcga aggtggcgag
      481 cgtcgcgagc gcgaacatcg cgacaagccg cgcggtgatg gagtgcctca cgcggcctct
      541 ccgtcggtgg gctcgtcgcg cacctccata acgtagccca tgccccggat ggtatgcagc
      601 agcttcggcg cgaacgcggc gtcgatcttc gcgcgcaggc gcttgatcgc gacctcgacg
      661 acgttggtgt tgctgtcgaa gttcatgtcc caaacgagct ccgcgatcgc ggtcttggac
      721 aggatctcgc cctgcctgcg cgcgagcacg gccagcagcg cgaactcctt ggcgctcagg
      781 tcgatgcgct cgccgccgcg ccaggccttg cgcgcgatca ggtcgatctg caggttggcg
      841 atgcgcagct gcgccggctc ctggctgcgc ccgcggcgct ggagcgcctg cagccgggcg
      901 agcagctcga ggaacgagaa cggcttgacc aggtagtcgt ccgcgccgtc gtgcaggccg
      961 cgcacgcgat cctcgatcga gtcgcgcgcc gtcagcatga tcaccggcgt ctgcttgacc
     1021 gcccgcagcg cgcgcagcag cgtgaagccg tcgatgccgg gcagcatcgc gtcgagcacg
     1081 acgatgtcgt agtcgtgcac cagcgcgagg tgctggccct cgacgccgtc gtgcgcgacg
     1141 tcgaccgcgc agccctgctc ggtcaggccc ttgcgcaggt actcggcggt cttgagctcg
     1201 tcctcgacga tcagcagctt catggatgcg cccgattgtc cgcgacccgt cgccgcctca
     1261 gcgcggctcg ccgccggtgg gcaccggcag gcggcggtcg gggtcggtgc cgtccggggc
     1321 gaagcctccc gtgcccgggc cgccgtcctt gcccatcttc gccttgcgct tcgcgcgcgc
     1381 ctccttgcgc ttcatgtacc agtagtgcgc gcggtcgagg tacaggtaga tcacgggcgt
     1441 cgtgaacagc gtcagcacct gcgacagcac cagcccgccc accatcgcga agcccagcgg
     1501 ccgccgcagc tccgagcccg cgccgtgccc cagcatcagc ggcaggcccg acagcagcgc
     1561 gcacatcgtc gtcatcatga tcgggcggaa gcgcagcagg caggcccggt agatcgcctc
     1621 ctgcggcgtc aggccctggt cgcgctccgc ggtgagcgcg aagtccacca tcatgatgcc
     1681 gttcttcttc acgatgccga tcagcaggat gatgccgatc agcgcgatga ccgagaggtc
     1741 gtagccgccc gccatgagga tcagcagcgc ccccacgccg gccgagggca gcgtcgacag
     1801 gatggtgacg gggtggatga agctctcgta gagcagcccg aggacgatgt agaccgccac
     1861 caacgccgcc gcgatcaggt agggctgcgt gcgcagcgag tcgcggaagg cctgcgccgt
     1921 gccctggaac tggccgttga gcgcctgcgg caggcccatc tgcgcctgcg cctggttgat
     1981 cgcctcgacc gccttgccca gcgacgctcc cggcgcgagg ttgaaggaga tcgtgaccgc
     2041 ggggaactgg ccctggtggc tgatcgcgag gtaggcggtc ttgtcggtgt cgaccttgac
     2101 gaagctcgac agcggcacct gccggccggt gatcggcgag gtcaggtaca gcttgtcgaa
     2161 caggctcggg tcctgctgaa gcttcggcgt cacctccagc accacgtggt agctgttgat
     2221 ctgcgtgaag tactggttga cctggcgctg gccgatcgcg tcgtagatcg tcgcgtcgat
     2281 cagcgagggc gagatgccga agctcgcggc gcggtcgcgg tctatcgtca gggacgcggt
     2341 ggccgcggcg ttctgcatgt cggaggcgac gtcggtcagc tgcggcagct tctgcaagcg
     2401 ctccagcagc ttcggtgccc attcgttgag ctcgtcgatg ttggcgtcgg tgagcgtgta
     2461 ctggtactgc gtgcgcgcca ggcggccgcc gacgttgatg tcctggcccg cctgcatcag
     2521 cagcgaggcg ccgccgacct tggcgagctt cggacgcagg cgcgcgatga tctggtcggc
     2581 gctcagcctg cggccctggt ccttcggctt gagcacgatg aagaagttgc cggtgttgaa
     2641 ctgcgacgag ttgccgaaca tgcccacgcc ggtgacgtcc ggatcctggc ggatgatgtc
     2701 ggcgaactgg atctggcgcc ggttcatcga ctcgaacgac gagtcctgcg aggactgcgc
     2761 gttgccgaag atgaaaccgg tgtcctgctg cgggaagaag cccttgggga tcgcgatgaa
     2821 cagcagcacc gtcgccgtca ccgtcgcgag gaagctcagc agcgtgatga actggtggcg
     2881 catcacgacg tccagcccgc ggcggtaggc gcccagcatc cggtcgaagc ccgcctcgaa
     2941 cagctggtac aggcggccgt gcctcttcgc gtgctcgtcc ttcaggaaac gcgagcacag
     3001 catcggcgtg agcgtcagcg agacgatcac cgacacgccg atcgtcagcg tcaccgtgac
     3061 cgcgaactcg cggaacagcc ggcccacgat gccgcccatc agcagcagcg ggatgaacac
     3121 cgccaccagc gacaccgaga tcgagatgat cgtgaagccg atctcgccgg cgcccttgat
     3181 cgccgcctcc atcggcggca tcccctcctc gacatggcgg tagatgttct ccagcatcac
     3241 gatggcgtcg tcgacgacga agccgaccgc gatggtcagt gccatcagcg agaggttgtc
     3301 gaggctgtag ccgacgacat acatgatcgc cgccgtgccc agcagggcga gcggcaccgt
     3361 gacgctcggg atcagcgtgg ccgccacgtt gcgcaggaag atgaagatga ccatcacgac
     3421 cagcgcgatc gtcagcagca gcgtgaactc gacgtcgtgc accgaggcgc ggatggtctg
     3481 cgtgcggtcg atcagcgtgt cgacgtgcac ggtcggcggg atcgcggcgc gcaggcgcgg
     3541 cagcgcctcc ttgaccttgt cgaccgtctc gatgacgttg gcgcccggct gcttgtagat
     3601 gatcagcagc atcgcgcggc cgttgccgac gctgtcgggc gccgcggcgc cggagaacgc
     3661 ccacgcgtgg accttgctgt cctcgggcgc gtcgacggcc acgccgatgt cgcgcacgcg
     3721 caccggcgcg ccgttgcggt aggcgagcac gacgtcgttc cacggcgccg ccttgagcaa
     3781 ctggtcgttg gtgtagaccg tgaagctctg gctcgggccg tcgatcgtgc ccttgggcgc
     3841 gttgaccgac gtcgtcgcga tcaccccgcg cacgtcctcc aggctcatgc ccatcgaggc
     3901 gagcttggcc ggatcgacct gcacccgcac cgccggcttc tgcgcgccgc cgatgttgac
     3961 gaggcccacg ccggtgatct gcgagatctg ctgcgcgagg atgttgtcgg cgtagtcgtt
     4021 ggtctggatc agcggcagcg tgtccgagtg caccgccatg atcatgatcg gtgaatcggc
     4081 ggggttgacc ttgcggaacg tcggcgggct gggcaggttg gtcggcagct ggccggtggc
     4141 cgcgttgatg gccgcctgca cgtcgagcgc ggcgccgtcg atcgcgcggt tcaggtcgaa
     4201 ctgcagcgtg atctgcgtcg agccgatcgc gttcgtcgac gtcatctgcg tgaggccggc
     4261 gatcagcgag aactgccgct cgagcggctg cgcgacgttg gaggccatgg tctccgggct
     4321 cgcgccgggc agcttggccg agacctggat ggtcgggaag tcgacctggg gcagcggagc
     4381 caccggcagc agcgggtaga ccgcgatgcc gaccagcagg atcgcgagcg ccagcagcgt
     4441 cgtgccgatg ggccgcttga tgaagccttc ggagacgctc acttgccgct cccggagtca
     4501 ccctggccgg aggtgtcgcg cttgccgtcg gaggcggcgc ccgcgggggc ggacgcgccg
     4561 cgcgccggcg cctcgacggt gcgcgacccc ggcttgagct tgtactggcc gtcgaccacc
     4621 acgcgctggc cgggctgcag gccctggtcg acgatggcga cgccgtcctg gatctgcacg
     4681 aggccgatgg tctgcgcgcg tgcgctgccg cccgggtcga ccacgtaggt gaagacgccg
     4741 ctctcgctgc gctgcaccgc ggcggcgggg atcgtgatcg cctgccggcg cgtgcccagc
     4801 accagccgca cgttgacgaa ctggcccggc cacagcgcgt gctgaggatt ggggaagctg
     4861 cccttcatct gcaccgtgcc gctggtggtg tcgatctggt tgttgacgag cgtgagcgtg
     4921 ccggtgccca gcagctcgct gccgtcgcgc gaccaggcct gcacgaccag cggcgcgtgc
     4981 gatgcggcca tcgcgcggtt ggtggcctgc acggcgtcct cgggcagcgt gaacgccacc
     5041 gtgatcgggt cgatctggtt gatcaccacg aggccgctcg cgtccgcggc atgcacgatg
     5101 ttgcccggat cgaccaggcg cgcgcccgcg cggccggaga tcggtgccgc gatcgtcgtg
     5161 tagcccagct gcacctgcgc cgaattgacc tgcgcgtcgt ccgacttcac cgtcgcctcg
     5221 agctgcttga cgagcgcctt ctgcgtgtcg agctgctgct gcgtggccgc gtcctgcgcc
     5281 gcgagcgtgg tgtagcgctg caggtcgacg cgcgcgttgg cgagcgtggc ctcgtccttc
     5341 gcgcgctgcg cctgcgcctg catcagcgtc gcctggaagg tgcgcgggtc gatgcgggcg
     5401 atcacctggc ccttcttcac gtcctggcct tccgcgaagc cgaccgcgtc gagctggccg
     5461 tccacgcgcg ccttgaccgt gaccgaataa gtggcagcga cggtgcccag gcccgtgcgc
     5521 aggatgggca tgtcctgcgc cttcacgggc gcggtgacga cctgcaccgg cgcgtcgtcg
     5581 gccttgcgct tcgcgtcctt gtcggcggct tgggccgagt cgtgcgagag cgcccagagg
     5641 ccggcggccg cgacgacgag cgtggcggcg gaggcgatcg cccaccgggc gcgcgaatcg
     5701 aaggggaggg tcttcatgtc gtcggctctt ggatcgtgcg cgcctgggcg gcggtggagg
     5761 cagcggggac ggtggcggag ggaacggcgt cgggacgatc ggcgcgccag ccgccgccgg
     5821 tcgcggcgat caggctcacg ctcgcggcga gctggcgccc gcgcagctgc acggccgcgc
     5881 gctgcgcgcc cagcgacagc tgctgcgccg tcacgacgtt gaggtagctg ttggtgccgg
     5941 cgcggtactg cagcagggcg aggcgctcgg aggtctgcgc cgcgcgcacg gcttcgtcct
     6001 ggtagccgga ctcgcgggcg agctcgcgca gcgtggcgag ctggtcctcg acctgctgga
     6061 agcccgacag caccgtctgc ttgtactgcg cgacggcagc gtcgtacgcg gcctcggcct
     6121 gcgccgtgcg ggcgcgccgc agcccgccgt cgaacagcgc ctgcgcaagc gccgcgccca
     6181 gcgaccatac gcgcgccggc gtgtcgaaca gctgcgacat ctgcgccgcc gcgccgccgc
//
LOCUS       DASVLN010000515         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_457566, whole genome shotgun sequence.
ACCESSION   DASVLN010000515 DASVLN010000000
VERSION     DASVLN010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457566"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF453_12635"
     CDS             complement(<1..203)
                     /locus_tag="VF453_12635"
                     /inference="COORDINATES: protein motif:HMM:NF016161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEX7688551.1"
                     /translation="MRPSSRGRASTDAKAENMDYLALRAFHETTVAVSVTGFAARAAA
                     GLAGARWSRGRLARTLPHVVDTAL"
     gene            complement(157..1356)
                     /locus_tag="VF453_12640"
     CDS             complement(157..1356)
                     /locus_tag="VF453_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX7688552.1"
                     /translation="MAILLPLTEPRRGPDAEATGFALWRLGFRPFYLFASAFGAVSVA
                     CWALQASGLSAHAYLRGPLWHAHEMLFGFVFAVVVGFLFTAGRNWTGQPTPTGRALMA
                     MVALWAAGRILVLTPFAVTAAAVNVAFALVAAGALARPLVASGNRRNYFFVAVLAAMA
                     LAVLAVHLDQLGVAPGIGWPGIRLGLDLVLFIVSVMTGRILAMFTNNGVPGAGARRVD
                     WLEKLASGSVLALAMADVLALPAPAVAAIAACAAVVHGARFALLRSWRVLREPVVWVL
                     HGATAWIPVHLALRALSALGAIPASAATHALTVGVLGGLVIGMITRTARGHTGRPLRG
                     GWTETAMYALVLAAAPVRVFLPLVSPGLAIVAIEASAGLWVAGFALYAIRYATILARP
                     RVDGREG"
     gene            complement(1356..>2193)
                     /locus_tag="VF453_12645"
     CDS             complement(1356..>2193)
                     /locus_tag="VF453_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688553.1"
                     /translation="GGRQAAGALMLVVAAACALGTNILIARRQPAAHTALLVLAAAAW
                     LAGNALFLAGAGAAIPAWFAFLVLTIAAERLEMTRLTRRGRTTQAELLAIVALMLAGA
                     GLASTGGAGSTAGLACFGASLALLSCWLFARDVATRTLRGHGLARYMAVCLLLGYGWL
                     AAAGLGWAGLAFGRPWRDLALHALGLGFVVSMMMAHAPVILTALTGLRVRYGAAFYLP
                     VALLQASLCVRLGPGLDDLLWRGIGAFLNAAALLAFAATLAIGTARSRARRPATPLME
                     TP"
BASE COUNT          352 a          833 c          819 g          189 t
ORIGIN      
        1 agggcggtgt cgacgacgtg cggcagcgtc cgcgcgagcc ggccccggct ccagcgcgcg
       61 ccggcaagcc ccgcggcggc gcgggcggcg aagccggtca ccgagaccgc gaccgtggtt
      121 tcgtggaatg cgcggagggc aaggtagtcc atgttctcag ccttcgcgtc cgtcgacgcg
      181 cggccgcgcg aggatggtcg catagcggat cgcgtacagc gcgaagcccg cgacccacag
      241 cccggccgag gcctcgatcg cgacgatcgc gagcccgggg ctcacgagcg gcaggaagac
      301 ccgcaccggc gccgccgcca ggacgagcgc gtacatcgcc gtctcggtcc agccgccgcg
      361 cagcggccgt ccggtatgac cgcgcgccgt gcgcgtgatc atgccgatga cgagtccgcc
      421 cagcacgccc accgtcagcg catgcgtcgc cgcggacgcg gggatcgcgc cgagcgccga
      481 cagcgcgcgc agcgcgaggt ggaccgggat ccaggcggtc gcgccgtgca gcacccacac
      541 gaccggctcg cgcagcacgc gccacgagcg cagcagcgcg aagcgcgcgc cgtggaccac
      601 cgcggcgcac gcggcgatcg cggcgaccgc cggcgccgga agcgcgagca cgtccgccat
      661 cgcgagggcc aggacgctgc cggaggcgag cttctcgagc cagtccacgc gccgggcgcc
      721 ggcccccggc acgccgttgt tcgtgaacat ggcgaggatg cgtcccgtca tgaccgagac
      781 gatgaacagc accaggtcga ggcccagccg gatgccgggc cagccgatcc ccggggcgac
      841 gccgagctgg tcgagatgca ccgccaggac ggcgagcgcc atcgccgcga gcaccgcgac
      901 gaagaagtag ttgcgccggt tccccgaggc gacgagggga cgcgcgagcg cgcccgccgc
      961 caccagcgca aacgcgacgt tcaccgccgc ggcggtcacc gcgaagggcg tcagcaccag
     1021 gatgcggccc gcggcccaca gcgccaccat cgccatcagc gcgcggccgg tcggcgtcgg
     1081 ctggccggtc cagttgcgcc ccgcggtgaa caggaaaccg acgaccacgg cgaacacgaa
     1141 cccgaacagc atctcgtgcg catgccagag gggcccgcgc aggtaggcgt gcgccgacag
     1201 gcccgacgcc tgcagcgccc agcacgccac cgacacggcg ccgaacgcgc tcgcgaacag
     1261 gtagaacggc cggaagccga gccgccacag cgcgaagccg gtggcctcgg cgtcggggcc
     1321 gcgccgcggc tcggtgaggg gcagcaggat cgccatcacg gggtctccat caagggggtc
     1381 gcggggcggc gggcgcggct tctcgcggtg ccgatcgcca gggtggctgc gaaggccagg
     1441 agcgcagcgg cgttcaggaa cgcgccgatg ccgcgccaca ggaggtcgtc caggcccggc
     1501 ccgagccgca cgcacaggga cgcctgcagc agggcgacgg gcaggtagaa cgccgcgccg
     1561 taccggaccc gaaggcccgt caaggccgtc aggatcaccg gcgcgtgcgc catcatcatg
     1621 ctcacgacga agcccaggcc cagcgcatgc agcgccaggt cccgccacgg ccggccgaag
     1681 gcgagaccgg cccagccgag gccggcggcc gccagccacc cgtagccgag cagcaggcag
     1741 acggccatgt agcgcgccag gccgtggccg cggagcgtgc gcgtcgcgac gtcgcgcgcg
     1801 aacagccagc acgacagcag cgccaggctc gcgccgaagc aggcgagtcc ggcggtcgag
     1861 ccggcgccgc cggtcgacgc gagtccggcg ccggcgagca tcagtgcgac gatcgccagc
     1921 agttcggcct gcgtcgtccg cccgcggcgc gtgagccggg tcatctccag gcgctctgcc
     1981 gcgatggtga gcaccaggaa ggcgaaccac gccggtatcg ccgccccggc cccggccagg
     2041 aacagcgcat tgccggcgag ccacgccgct gccgcgagca cgagcagggc ggtgtgcgcc
     2101 gccggctgcc gccgtgcgat caggatgttc gtccccagcg cgcacgcggc cgcgacgacc
     2161 agcatcagcg cgccggccgc ctgccggccg ccg
//
LOCUS       DASVLN010000516         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_348559, whole genome shotgun sequence.
ACCESSION   DASVLN010000516 DASVLN010000000
VERSION     DASVLN010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348559"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VF453_12650"
     CDS             complement(<1..246)
                     /locus_tag="VF453_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7688554.1"
                     /translation="MPSSSMPVLSVRGLDKHFTLHLRGGARLQVLRDAALDVAAGECV
                     ALVGPSGRGKSTLMKCLYGSYGADAGTLRFASAAGAVD"
     gene            complement(255..1055)
                     /gene="phnK"
                     /locus_tag="VF453_12655"
     CDS             complement(255..1055)
                     /gene="phnK"
                     /locus_tag="VF453_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HEX7688555.1"
                     /translation="MTEPKSPEPLLRVSGLSHRFACAREDQLAVRDAALELHAGEVVA
                     IVGESGSGKSTLLNCIAGRLRPTAGSVHYRDAAGRWLDVHALSEREQRVLARTEWGFV
                     EQAASDGLRLDVSAGANIGERLMGQGERHYGRLRDAAERWMRRVELDPARIDDSPRAF
                     SGGMRQRLQIARNLVTRPRLVLLDEPTSGLDVSVQARLLDLIRLLTARMHLCALIVTH
                     DLGVARLLSTRTLVMRQGRIVEQGLTDRVLDDPQHPYTQLLVSSVVMP"
     gene            complement(1048..1905)
                     /locus_tag="VF453_12660"
     CDS             complement(1048..1905)
                     /locus_tag="VF453_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HEX7688556.1"
                     /translation="MTAEGYNFAYLDERTKKMIRRALLKAVAIPGHQVPFGSREMPLA
                     YGWGTGGIQVTAAVIGRDDVLKVIDQGSDDTTNAVNIRQFFARTTGVRTTTRTGEATL
                     IQTRHRIPERPLVEGQVLVYQVPQPEPLYRLEPRRAETAKLHAAEEYGLMNVKLYEDL
                     AQWGRIETSYDYPVRVDGRYVMSPSPIPAFDNPKMHRNPALQLFGAGREKRLYAIPPY
                     TDVASLDFEDHPFRIEPHGHACERCGATGTFLDEVQAAPGQTRWTCSDTDWCAERVDA
                     LHSREPAHD"
     gene            complement(2018..3151)
                     /locus_tag="VF453_12665"
     CDS             complement(2018..3151)
                     /locus_tag="VF453_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HEX7688557.1"
                     /translation="MYIAVKGGERAIEASRRLLDAARRGDPAVPPIEVAQVRQQMGLL
                     VGRVMAEASLYDPELAALALVQACGDAIEAAFLLRAYRTTLPRLAYSEPIDTDRMRVE
                     RRVCAIFKDLPGGQLLGPTFDYTQRLLDFDLLRPRRDVPASASTQSTTPDAEAEADAA
                     VPNALAQLAAEGLVEPCLPSPGDPEPPDITRDPPAYPLPRAGWLQMLARADEGWVLGM
                     GYSTQRGYAHTHPFAGDIRSGRVTVEVVPDELDFAIEIGEVEVTECQMVNQFVGSAGV
                     PPVFTRGHGLVFGRGERKVIAMSLVDRALRAAEFGEDAASPAQQQEFALPQGDSVEAS
                     GFVQHLKLPHYVTFESELQLIRKLREDAQARAQADFDAEGASE"
     gene            complement(3153..>3440)
                     /locus_tag="VF453_12670"
     CDS             complement(3153..>3440)
                     /locus_tag="VF453_12670"
                     /inference="COORDINATES: protein motif:HMM:NF017645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HEX7688558.1"
                     /translation="LLARLALGTDESPQDGATLVVEVDGLDTAGGIALALRGPGVESV
                     QRLRVAGLPASFWDWRRDLQRQLPRGVDLVLVHGARVAAIPRSARLQIEGA"
BASE COUNT          455 a         1209 c         1297 g          479 t
ORIGIN      
        1 gtcgaccgcg ccggcggccg acgcgaagcg cagcgtgccg gcgtccgcgc cgtagctgcc
       61 gtacaggcac ttcatcagcg tcgacttgcc gcggcccgac ggtccgacga gcgcgacgca
      121 ttcgcccgcg gcgacatcca gcgcggcgtc ccgcagcacc tgcagccgcg cgccgccgcg
      181 caggtgcagc gtgaagtgct tgtccaggcc gcgcacggac agcaccggca tcgaggaaga
      241 gggcatggcg ttcatcatgg catcacgacg gaggagacga gcagctgcgt gtagggatgc
      301 tgcgggtcgt cgagcacgcg gtcggtcagg ccctgctcga cgatccggcc ctggcgcatc
      361 accagcgtgc gcgtgctgag caggcgcgcc acgccgaggt cgtgcgtgac gatcagcgcg
      421 cacaggtgca tacgggcggt gagcaggcgg atcaggtcga gcagccgcgc ctgcaccgac
      481 acgtccaggc cggaggtggg ctcgtccagc agcaccaggc gcggccgcgt gaccaggttg
      541 cgggcgatct gcaggcgctg gcgcatgccg cccgagaagg cgcgcggcga gtcgtcgatg
      601 cgcgccgggt cgagctcgac gcgccgcatc cagcgctctg ccgcgtcgcg cagccggccg
      661 tagtggcgct cgccctgtcc catcaggcgt tcaccgatgt tggcgccggc gctgacgtcc
      721 aggcgcagcc cgtcgctggc ggcctgctcg acgaagcccc actcggtgcg tgcgagcacg
      781 cgctgctcgc gttcgctcag cgcatggaca tcgagccagc gccccgcggc gtcgcggtag
      841 tgcacgctgc cggcggtcgg gcgcaggcgg ccggcgatgc aattcagcag cgtcgacttg
      901 cccgagccgg actcgccgac gatggccacg acctcgccgg catgcagctc gagcgcggcg
      961 tcgcgcacgg cgagctggtc ctcgcgcgcg caggcgaagc ggtgcgacag gccgctcacg
     1021 cgcagcagcg gttcggggga cttgggctca gtcatgggca ggctcgcggg agtgcagggc
     1081 gtcgacgcgc tccgcgcacc agtcggtgtc ggagcaggtc catcgggtct ggcccggcgc
     1141 ggcctggacc tcgtcgagga aggtgccggt cgcgccgcag cgctcgcagg cgtggccgtg
     1201 cggctcgatg cggaacggat ggtcctcgaa gtcgaggctg gcgacgtcgg tatagggagg
     1261 gatcgcgtag agccgcttct cgcgtccggc gccgaacagc tgcagcgccg ggttgcggtg
     1321 catcttcggg ttgtcgaacg cggggatggg cgatggcgac atcacgtagc gcccgtcgac
     1381 ccggacgggg tagtcgtagc tcgtctcgat gcggccccac tgcgcgaggt cctcgtagag
     1441 cttgacgttc atcagcccgt attcctcggc cgcgtgcagc ttcgcggtct cggcgcggcg
     1501 cggctcgagg cggtacagcg gctcgggctg cggcacctgg tagacgagca cctggccctc
     1561 gaccagcggc cgctcgggaa tgcggtggcg cgtctggatc agcgtcgcct cgcccgtgcg
     1621 cgtggtcgtg cgcacgccgg tcgtgcgggc gaagaactgg cggatgttga cggcgttggt
     1681 cgtgtcgtcg ctgccctggt cgatcacctt gagcacgtcg tcccggccga tcaccgccgc
     1741 cgtcacctgg atgccgccgg tgccccagcc gtaggcgagg ggcatctcgc gcgagccgaa
     1801 gggcacctgg tggcccggga tcgcgaccgc cttcagcagc gcgcgacgga tcatcttctt
     1861 cgtgcgttcg tcgaggtagg cgaagttgta tccctcggca gtcatgcgat cccccagtcg
     1921 gcggagtgcc gtcgccagtc ccacggcgcg cccggccttg ctccgcagcg gccgagcgcg
     1981 gcggccgggc gctcgcggcc ttcgttcgac acatcgatca ttcggacgct ccttcggcat
     2041 cgaagtcggc ctgggcgcgc gcttgcgcgt cctcgcgcag cttgcggatc agctgcagct
     2101 ccgactcgaa agtgacgtag tgcggcagct tcaggtgctg cacgaagccg gaggcctcga
     2161 cgctgtcgcc ctgcggcagc gcgaactcct gctgctgcgc ggggctcgcg gcgtcctcgc
     2221 cgaattcggc ggcgcgcagc gcgcggtcca cgagcgacat cgcgatcacc ttgcgctcgc
     2281 cgcggccgaa gaccaggccg tggccgcgcg tgaacaccgg cggcacgccg gcgctgccca
     2341 cgaactggtt gaccatctgg cattcggtga cctcgacctc gccgatctcg atcgcgaagt
     2401 cgagctcgtc gggcaccacc tccacggtca cgcgcccgct gcggatgtcg cccgcgaacg
     2461 gatgcgtgtg cgcgtagccg cgctgcgtcg aatagcccat gcccaggacc cagccctcgt
     2521 cggcgcgcgc gagcatctgc agccagccgg cgcgcggcag cgggtaggcg ggcgggtcgc
     2581 gcgtgatgtc gggcggctcc gggtcgccgg gcgagggcag gcagggctcg accaggcctt
     2641 cggcggcgag ctgggcgagg gcgttgggca ccgcggcgtc ggcctcggcc tcggcgtcgg
     2701 gggtggtcga ctgcgtggac gcggaggccg ggacgtcgcg gcggggccgc agcaggtcga
     2761 agtccagcag gcgctgcgtg tagtcgaagg tcgggcccag gagctggccg ccgggcaggt
     2821 ccttgaagat cgcgcagacg cggcgctcga cgcgcatgcg gtcggtgtcg atcggttcgc
     2881 tgtacgcgag gcgcggcagc gtcgtgcggt aggcgcgcag caggaaggcg gcctcgatcg
     2941 cgtcgccgca ggcctgcacc agcgcgagcg cggcgagctc ggggtcgtac agcgaggcct
     3001 cggccatcac gcgtccgacc agcaggccca tctgctggcg cacctgcgcg acctcgatcg
     3061 gcggcaccgc cggatcgccg cggcgcgcgg cgtccagcag ccggcgcgag gcctcgatcg
     3121 cgcgctctcc gcccttgacg gcgatgtaca tgtcaggctc cttcgatctg caggcgcgcg
     3181 ctgcgcggga tcgcggcgac gcgcgcgccg tgcaccagca ccaggtcgac gccgcgcggc
     3241 aactgccgct gcagatcgcg ccgccagtcc cagaacgagg ccggcaggcc cgccacgcgc
     3301 aggcgctgca cggactcgac gcccgggccg cgcagcgcga gtgcgatacc gcctgccgta
     3361 tcgagtccgt cgacctcgac gaccagcgtc gcgccgtcct gaggcgactc gtccgtgccg
     3421 agcgcgaggc gggcgagcag
//
LOCUS       DASVLN010000517         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_511925, whole genome shotgun sequence.
ACCESSION   DASVLN010000517 DASVLN010000000
VERSION     DASVLN010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511925"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="VF453_12675"
     CDS             <1..1367
                     /locus_tag="VF453_12675"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEX7688559.1"
                     /translation="AAAALALASGWIAGGLGDPLARNPVLVAMLCGLLVNALFGCPQA
                     LRPGLDFTRRTLLRAGVALLGLRITVALLGALGPVPIVIAATELLAVLVGVRWIARRL
                     LRLDDELALLVAVGSAVCGAAAILSFAAMSRDRERHAATAVTLITLAGTLALLAYPVA
                     YLAGWLPALDEHGFGVTVGASIFELAQVYGASASVSEGALGVATLVKLSKVLMLVPLM
                     LAAGWGARDGAAGARRARVQVPWFVVGFIAVVLLNSAVTVHPLVRATLLGASQFMFMM
                     TMVALGLCTRVDALRDWRLVAAGAVALALSSGIAYGLVALSGGLAAPSAGPGAPGSRP
                     TASSTSAGARLFDDVGCSKCHVRALHGAHGDVVVYSDLLLHDMGPALDDKIVQGDATG
                     ADWRTTPLVGLSTHARYLHDGRAADLRAAIFDHGGEAAVVRERFFELEPAEQAQVYAF
                     LRTP"
BASE COUNT          160 a          548 c          553 g          242 t
ORIGIN      
        1 cggcggcggc cgcgctcgcg ctggcctccg gctggatcgc cggcggcctg ggcgacccgc
       61 tcgcgcgcaa ccccgtgctc gtcgcgatgc tgtgcggact gctggtcaac gcgctgttcg
      121 gctgtccgca ggcgctgcgg ccgggcctcg acttcacgcg ccgcacgctg ctgcgcgcgg
      181 gcgtcgcgtt gctgggcctg cgcatcaccg tggccctgct gggcgcgctc ggccccgtgc
      241 cgatcgtcat cgcggccacg gagctgctcg cggtgctggt cggcgtgcga tggatcgccc
      301 gccggctcct gcgcctggac gacgagctcg ccctgctggt ggccgtcggc tcggcggtct
      361 gcggcgcggc cgcgatcctg tccttcgcgg ccatgtcgcg cgaccgggaa cggcacgcgg
      421 cgacggcggt caccctgatc acgctggcgg gaacgctcgc gctgctggcg taccccgtgg
      481 cgtatctcgc cgggtggctg ccggcgctcg acgagcacgg cttcggcgtg acggtgggcg
      541 cgagcatctt cgagctcgcg caggtctacg gtgcctcggc gagcgtctcc gagggcgcgc
      601 tgggcgtggc caccctggtc aagctcagca aggtcctgat gctggtgccg ctgatgctgg
      661 cggccggatg gggcgcccgc gacggcgcgg ccggcgcacg cagggcacgc gtgcaggtgc
      721 cgtggttcgt cgtcggcttc atcgccgtcg tgctcctcaa ttccgcggtc accgtgcatc
      781 cgctggtgcg tgccacgctg ctgggagcca gccagttcat gttcatgatg accatggtgg
      841 cgctgggcct gtgcacccgc gtcgacgcgc tgcgcgactg gcgcctggtc gccgccggcg
      901 ccgtcgccct cgccctgtcg tcgggcatcg cctacgggct ggtcgccttg agcggcgggc
      961 tcgcggcccc gtcggccggg ccgggcgccc ccggcagccg tccgacggcg tcgtccacgt
     1021 ccgcgggcgc gcggctgttc gacgacgtgg gctgcagcaa gtgccacgtc cgcgcgctgc
     1081 acggcgcgca tggcgatgtc gtcgtgtatt ccgacctgct gctgcacgac atgggcccgg
     1141 ccctcgacga caagatagtc cagggcgacg cgaccggcgc ggactggcgc acgacgccac
     1201 tcgtggggct gtcgacccat gcccgctacc tgcacgacgg ccgcgcggcc gacctgcgcg
     1261 ccgccatctt cgatcatggc ggcgaggccg cggtcgtccg cgagcgcttc ttcgagctcg
     1321 agccggccga acaggcgcag gtctatgcgt tccttcgcac gccctgagag ggtgtagctg
     1381 aaatgatcta cgcctgcgcc aatcgcgaag cgagaaggcg ggcctcggtc agccacaccc
     1441 atgcgacggg cgcccagttg gagcgatcgt gatggaccat caatcggcgc gctcgctcat
     1501 tcc
//
LOCUS       DASVLN010000518         2393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_457739, whole genome shotgun sequence.
ACCESSION   DASVLN010000518 DASVLN010000000
VERSION     DASVLN010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457739"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VF453_12680"
     CDS             <1..475
                     /locus_tag="VF453_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX7688560.1"
                     /translation="WARRDLVRVTALNDFVVLTRETRELRLQAEQMGRSLVEWLRNQD
                     ASDGRLAHLAALAPSPTWPVAFGLATARAGATAAQALQACLFGWAENLVQAALKAVPL
                     GQLAGQRILQSLLARMPALVDAALVLDDGDWQAFSPMLGIASARHETQYSRLFRS"
     gene            496..1167
                     /gene="ureG"
                     /locus_tag="VF453_12685"
     CDS             496..1167
                     /gene="ureG"
                     /locus_tag="VF453_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEX7688561.1"
                     /translation="MTDPAPTRPSPLHHIPGRTRRRPPLRVGVGGPVGSGKTTLVEML
                     CKAMRDKWDLVVVTNDIYTKEDQRLLTVAGALAPERIMGVETGGCPHTAIREDASVNL
                     EAIDRMLEKFPDADIVFVESGGDNLAATFSPELSDLTIYVIDVAAGEKIPRKGGPGIT
                     KSDLFVINKTDLAPHVGADLAVMEADTRRMRPGRPHVMTNLKTKAGLDVVVAFIEQRG
                     MLGAG"
     gene            1309..2343
                     /locus_tag="VF453_12690"
     CDS             1309..2343
                     /locus_tag="VF453_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX7688562.1"
                     /translation="MPQLMRAAVVHAFGQPLEIEEVPVPEVPPGRILVKVVASGVCHT
                     DLHAASGDWPVKPALPFIPGHEGVGHVAAVGAGVRNVREGDRVGVPWLHTACGCCEHC
                     LTGWETLCDEQQMTGYTVNGGYAEYVLADPAYVGRLPASVPFPEIAPILCAGVTVYKG
                     LKMLDCKPGDWVAVSGVGGLGHLAVQYAKAMGFHVIGVDVDEAKLALARRLGADMVVD
                     ASTTDAAAEVQRALRGAHGVLVTAASRPAFDQGLAMLHKRGTMALVGLPPGEFGLPIF
                     DVVLNAKTVRGSIVGTRKDLQEALDFAGEGKVRAVWQEARLPDINAVFDRMRRGDIEG
                     RIVMRIGEDG"
BASE COUNT          322 a          912 c          841 g          318 t
ORIGIN      
        1 gtgggcgcgc cgcgacctgg tgcgcgtcac ggcgctcaac gacttcgtcg tcctcacccg
       61 cgagacccgc gagctgcgcc tgcaggccga gcagatgggc cgctcgctgg tcgaatggct
      121 gcgcaaccag gacgcgtccg acggccgcct cgcgcacctc gcggccctgg cgccctcccc
      181 gacctggccc gtggccttcg gcctcgcgac ggcgcgtgcc ggcgcgaccg cggcgcaagc
      241 gctgcaggcc tgcctgttcg gctgggcaga gaacctcgtg caggcggcgc tgaaggcggt
      301 gccgctgggg cagctggccg gccagcgcat cctgcagtcg ctgctcgcgc gcatgcccgc
      361 gctggtcgac gccgcgctcg tgctggacga cggcgactgg caggccttct cgccgatgct
      421 gggcatcgcg agcgcgcggc acgagaccca gtactcgcgc ctgttccgat cctgaaccct
      481 cgacccgatc ccgccatgac cgatcccgcc ccgacccgcc cgagcccgct gcaccacatc
      541 cccggccgca ccaggcgccg gccgccgctg cgcgtgggcg tcggcggccc ggtcggctcc
      601 ggcaagacga cgctggtcga gatgctgtgc aaggcgatgc gcgacaaatg ggacctcgtc
      661 gtcgtcacca acgacatcta caccaaggag gaccagcgcc tgctgacggt cgccggcgcg
      721 ctggcgcccg agcgcatcat gggcgtcgag accggcggct gcccgcacac cgcgatccgc
      781 gaggacgcct cggtcaacct cgaggcgatc gaccgcatgc tggagaagtt ccccgacgcg
      841 gacatcgtct tcgtcgagtc cggcggcgac aacctcgccg ccaccttcag ccccgagctg
      901 agcgatctca cgatctacgt gatcgacgtc gccgccggcg agaagatccc gcgcaagggc
      961 gggccgggca tcacgaagag cgacctgttc gtcatcaaca agaccgacct ggcgccccac
     1021 gtgggtgcgg acctggccgt gatggaggcc gacacgcgcc gcatgcgccc cggtcggccg
     1081 cacgtgatga ccaacctgaa gacgaaggcc ggcctcgacg tcgtggtcgc cttcatcgag
     1141 cagcgcggga tgctgggcgc gggctgagcg cgcccggacc gcccgcggcg ccgccgctgc
     1201 gcatgccggc acggtctccc ggtcgacacc ttcccgcttg acgcgcgtca agccgcgcgg
     1261 cgtcgcggct tcctatcgtt cgcgcacgca tcgaaccagg agtccgccat gccccagctg
     1321 atgagagccg ccgtcgtcca cgccttcggg cagcccctcg agatcgagga ggtgccggtg
     1381 cccgaggtgc cgcccggccg catcctcgtg aaggtcgtcg cctcgggcgt ctgccacacc
     1441 gacctccatg ctgccagcgg cgactggccg gtcaagcccg ccctgccctt catcccgggt
     1501 cacgagggcg tcgggcacgt cgccgcggtc ggcgccggcg tgcgcaacgt gcgcgagggc
     1561 gaccgggtcg gcgtgccctg gctgcacacg gcctgcggct gctgcgagca ctgcctgacg
     1621 ggctgggaga cgctgtgcga cgagcagcag atgaccggct acaccgtcaa cggcggctat
     1681 gccgagtacg tgctggccga ccctgcgtac gtcgggcgcc tgcccgcctc ggtgccgttc
     1741 ccggagatcg cgcccatcct gtgcgccggc gtcacggtct acaagggcct gaagatgctc
     1801 gactgcaagc cgggcgactg ggtcgccgtg tccggcgtcg gcggcctcgg ccacctggcc
     1861 gtccagtatg cgaaggcgat gggctttcat gtcatcggcg tggacgtcga cgaggccaag
     1921 ctcgccctcg cgcgccggct cggcgcggac atggtcgtcg acgcctccac gaccgacgcg
     1981 gccgccgagg tccagcgcgc gctgcgcggc gcgcacggcg tgctcgtgac cgccgcctcg
     2041 cgcccggcct tcgaccaggg cctcgccatg ctgcacaagc gcggcacgat ggcgctcgtc
     2101 ggcctgccgc cgggcgagtt cggcctgccg atcttcgacg tcgtgctcaa cgcgaagacc
     2161 gtgcgcggct ccatcgtcgg cacgcgcaag gacctgcagg aggcgctgga cttcgccggc
     2221 gaaggcaagg tgcgcgccgt ctggcaggag gcccggctgc ccgacatcaa cgccgtcttc
     2281 gaccgcatgc gccgcggcga catcgagggc cgcatcgtga tgcgcatcgg cgaggacggc
     2341 tgacggccgc ggcgccgggc gccgggcgcc ggccgcgcgg cgcccgcgcc cgc
//
LOCUS       DASVLN010000519         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_457753, whole genome shotgun sequence.
ACCESSION   DASVLN010000519 DASVLN010000000
VERSION     DASVLN010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457753"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            310..1824
                     /locus_tag="VF453_12695"
     CDS             310..1824
                     /locus_tag="VF453_12695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX7688563.1"
                     /translation="MTTLTDTLPARAAGDQTFLGHPRGLFVIFATEMWERFSYYGMRA
                     ILVYYLTQSMGYQREDALALYGIYTALVYLTPILGGWLADRYLGLRKGAVVGATVMVL
                     GHFAMAVPSLLHVALGLLIAGNGFFKPNTSSMVGECYDGKDDPRRDGGYSIFYMGINL
                     GAFFSPLVCGTLGEKYGYHYGFAAAGVGMAIGLVTLVSLQGILGRAGLREGQSPVGAR
                     DVPGIAAWTIASVAVVYAVVYGWSPIGAFLHTLPTWVTTVIGLAMILGFLAIPVAKTH
                     TPAERSRVLAVCILVLFVIFFWMGFEQAGGTMSLFALKQTDRLIGGWEMPASWFQSIN
                     PLEIFIFAPAFAIAWGALNQSRFRLPDVAKQGLGMIVLGLGFIVLYVAQLKADRSGAV
                     GPQWLFFVYTLHTLGELMLSPIGLALTSRAAPHRIAGLLMGVWLLANGVANYYAGMLE
                     SLLKGSGIPPYLFLIGSSIGMGVLLLILTPVLNRMLERRDPGEETGVATYSTTA"
     gene            1896..2487
                     /locus_tag="VF453_12700"
                     /pseudo
     CDS             1896..2487
                     /locus_tag="VF453_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250735.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
     gene            complement(2489..2704)
                     /locus_tag="VF453_12705"
     CDS             complement(2489..2704)
                     /locus_tag="VF453_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688564.1"
                     /translation="MNPLADRSGKVADPGAPSRIETPMQVHPWTEDSPHAPRAEDADA
                     RYTGELFLPGWLRIERQPTAEQGPGGG"
BASE COUNT          409 a         1026 c          994 g          443 t
ORIGIN      
        1 cgccgcggcg gcgcgcgcgg gcggccatgc atcgagagcg gcggcagcga gggtcgcgcc
       61 gccggcgagg cgttgcagca gggttcgtcg atgcatggcg gatcggttcg cgcgggcgag
      121 ggaccgctgc ggcacgcgcc gtgcccgcac gggtaagccc cagggcggac aggctcggcg
      181 cggggattcg atttgcgaaa atccgcgttt cggcctttcc gggccagaga cggccgcacc
      241 gccgccgggc ccacgaggca cgaggcgccg ggcaggccat ttcctcctgc gacaccagag
      301 gattcctcct tgacgaccct caccgacacc ctgccggccc gcgcggccgg cgaccagacc
      361 ttcctcggac atccccgcgg cctgttcgtc atcttcgcca ccgagatgtg ggagcgcttc
      421 agctactacg gcatgcgggc gatcctcgtc tactacctga cgcagtccat gggctaccag
      481 cgcgaggacg cgctggccct gtacggcatc tacacggcgc tggtctacct cacgcccatc
      541 ctcggcggct ggctcgccga ccgctacctg ggcctgcgca agggcgcggt ggtcggcgcg
      601 accgtgatgg tgctgggtca tttcgcgatg gcggtgcctt cgctgctgca cgtcgcgctg
      661 ggcctgctga tcgcgggcaa cggcttcttc aagccgaaca cgagctcgat ggtgggcgag
      721 tgctacgacg gcaaggacga cccgcggcgc gacggcggct actcgatctt ctacatgggc
      781 atcaacctcg gcgcgttctt ctcgcccctg gtgtgcggca cgctgggcga gaaatacggc
      841 taccactacg gcttcgcggc cgcgggcgtg ggcatggcga tcggcctcgt gacgctcgtg
      901 tcgctgcagg gcatcctcgg ccgcgcgggc ctgcgcgagg gccagtcgcc ggtgggcgcg
      961 cgcgacgtgc ccggcatcgc ggcgtggacg atcgcgagcg tcgcggtggt ctacgccgtc
     1021 gtctacggct ggtcgcccat cggcgccttc ctgcacacgc tgccgacctg ggtcacgacc
     1081 gtcatcggcc tcgcgatgat cctgggcttc ctggccatcc cggtcgcgaa gacgcatacg
     1141 cccgccgagc gcagccgtgt cctggcggtg tgcatcctgg tgctgttcgt gatcttcttc
     1201 tggatgggct tcgagcaggc gggcggcacg atgtcgctgt tcgcgctgaa gcagaccgac
     1261 cgcctgatcg ggggctggga gatgccggcc agctggttcc agtcgatcaa cccgctcgag
     1321 atcttcatct tcgcgccggc gttcgcgatc gcctggggag cgctgaacca gtcgcggttc
     1381 cgcctgcccg acgtcgccaa gcagggcctg ggcatgatcg tgctgggcct gggcttcatc
     1441 gtgctgtacg tcgcgcagct gaaggccgac cgcagcggcg ccgtcggccc gcagtggctg
     1501 ttcttcgtgt acacgctgca cacgctgggc gagctgatgc tgtccccgat cggcctggcc
     1561 ctgacctcgc gcgcggcgcc gcaccgcatc gccggcctgc tgatgggcgt ctggctgctc
     1621 gccaacgggg tcgcgaacta ctacgcgggc atgctggaat cgctgctcaa gggcagcggc
     1681 atcccgccgt acctgttcct gatcggctcg tcgatcggca tgggcgtgct cctgctgatc
     1741 ctgacgccgg tgctcaatcg catgctcgag cgccgcgacc cgggcgagga gacgggcgtc
     1801 gcgacctact cgacgacggc ctgatacgcc tcgtcgacca acgcaaaggc ggccgctccc
     1861 ttctggccgc cacccacaaa tccaggagat cccgcatgca tcgcacgctc gccctgtcgt
     1921 ccgtcctgtc gttcgcggtc gcgctcgccg caccggccgc ccatgcggcg ccggtcacct
     1981 accaggtcga ccccacgcac acctacccca gcttcgaggc cgaccacatg ggcggcctgt
     2041 ccaagtggcc gcggaaagtt cgaccacagc aagggcacga tcacgatgga tcgcgaggcg
     2101 ggcaccggca cggtcgagat cacggtcgac atgaacagcg gcgacttcgg cctggcggcg
     2161 ctcgacaagg ccgcccgcag caaggaactc ttcgacagcg cgaagtaccc ccaggccgtc
     2221 ttcaagggca cgctggcgga cttcaaggac ggcctgccga ccaaggcgcc cggcgagctg
     2281 acgctgcacg gcgtgacccg gccggtcacg ctggacatcc gccatgccaa atgcatgccg
     2341 catccaatgc tcaagcgcga agtctgcggc gcggacgcct acgccaccct ccagcgagac
     2401 cagttcggca tgcccgtcgg caaggactac ggcttcgaca tggccgtcga cctgcgcatc
     2461 caggtcgagg cgatcgcgca gccgtgattc agccgccgcc cgggccctgc tcggccgtcg
     2521 gctggcgttc gatgcgcagc cagcccggga ggaacagctc gccggtgtag cgcgcgtcgg
     2581 cgtcctcggc gcgcggtgcg tggggcgagt cctcggtcca gggatgcacc tgcatcggcg
     2641 tctcgatgcg cgacggggcg ccggggtcgg cgaccttgcc gctgcggtcg gcgaggggat
     2701 tcatggcaag cctccaggtc gcggccgccg gagggccgcg tcactggcca gtgtgcggca
     2761 agcgccgcgc cttgtgccta aagaaaggcg gccggcgtca gggcagccgc acgcccgcga
     2821 gcggcacggc cacgcccagc agcagcgcca ggccgagcag cgcgaccgcc ca
//
LOCUS       DASVLN010000520         3228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_512225, whole genome shotgun sequence.
ACCESSION   DASVLN010000520 DASVLN010000000
VERSION     DASVLN010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512225"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VF453_12710"
     CDS             <1..1087
                     /locus_tag="VF453_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7688565.1"
                     /translation="DKAPAVDLVVGGQHVRIGVPANADADDVKEAIASAKDAIVEQLK
                     QQEKDRERADRAAARAAARAKSADADADADDDGGDADSDSDDVDSDGNRSTVRTHTVH
                     YGEMLPLLAVWWVLGSLLLKITYKGRMQAEVKAAAATETAEAESLRRQVLEAKMAMMQ
                     AQVEPHFLFNTLASIDHLIEFDPKRASQMQRNLISLLRASMPSMRSTDSGLRPLDLEL
                     AVVRPYLEILKVRMEERLTTEIDVPDGLLSAEFPPMMIQTLVENSIKHGLEPKAEGGH
                     LSVKAEIRHGKLAVTVADTGLGFEAAAQGTTHGTGVGLSNIRERLALLYGAKASLSIA
                     NNPVGGTIVTITVPYTAHAESAEGAPA"
     gene            1084..1632
                     /locus_tag="VF453_12715"
     CDS             1084..1632
                     /locus_tag="VF453_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688566.1"
                     /translation="MTDPHLDPTTGPRDDGLAYVPQPGDRPPPSTASGRAMRRKPSLS
                     GRIFRWWLAIAFLLALGAAACLAFGVGELHGGPLHITLEGSDLADGITIHGLSADGQA
                     ALSMAALLVAVLLLVLLPLILALVLALVACALVLALAAPLFGLAVALVVLTSPLWLVA
                     LCVWLVARSRRPPPPHSATIAA"
     gene            1629..2483
                     /locus_tag="VF453_12720"
     CDS             1629..2483
                     /locus_tag="VF453_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7688567.1"
                     /translation="MNAPTTDPTAPAAVRGVIADDERLMRDQLKARLAEVWPELQIVG
                     EAKNGLEAVELVAQHRPDVVFLDIRMPGLSGVDAARRIAQLPAREDDQDDEDDHALPE
                     IVFITAYDQYAIEAFEQGVADYVLKPAERERLGVTVARIKQRLALRGTEDEPPAPHLQ
                     QLLHKLSAKLNPGGTPPTLKWIQATVGQAIQMIPVEDVLFFISDEKYTRVQTATLEAL
                     IRKPIKELVDELDPDLFWQIHRSTLVNTHAIAGVTRDLRGRQIVSVKGHNEKLEVSRS
                     YTHLFKGM"
     gene            complement(2523..>3228)
                     /locus_tag="VF453_12725"
     CDS             complement(2523..>3228)
                     /locus_tag="VF453_12725"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HEX7688568.1"
                     /translation="AVPAPAPAPGSASPPAWRRALPVLGALCAALAIASAPWALGQPW
                     LNFAFKPLATLCVIAWAASAETDDAPVRRWIVVGLACSLAGDVALLWPVQGFLAGLVS
                     FLLGHLAYLVALTRRVRLAAVPLAFVAWALVAAGVLSFLWHGVPAQLRVPVVVYVTAL
                     SAMAAQASTAWRVRSDAPDAAKWGLVALGGALFVASDAVLAIDKFVGGVPMPTLWNLS
                     IYWLGQWLIARAAQKA"
BASE COUNT          512 a         1211 c         1126 g          379 t
ORIGIN      
        1 ggacaaggcg ccggcggtcg acctcgtcgt cggcggccag cacgtgcgca tcggcgtgcc
       61 ggccaacgcc gacgccgacg acgtcaagga ggccatcgcg agcgccaagg acgcgatcgt
      121 cgagcagctc aagcagcagg agaaggatcg cgagcgcgcc gaccgggccg ccgcgcgcgc
      181 cgcggcgcgg gcgaagtccg ccgacgccga tgccgatgcc gacgacgacg gcggcgatgc
      241 ggactcggac tccgacgatg tcgactccga cggcaaccgc agcaccgtgc gcacgcacac
      301 cgtgcactac ggcgagatgc tgccgctgct ggccgtctgg tgggtgctgg gctcgctgct
      361 gctgaagatc acctacaagg gccgcatgca ggccgaggtc aaggccgcgg ccgcgaccga
      421 gaccgccgag gccgagtcgc tgcggcgcca ggtgctcgag gccaagatgg cgatgatgca
      481 ggcgcaggtc gaaccgcact tcctgttcaa cacgctcgcg tcgatcgacc acctgatcga
      541 gttcgacccc aagcgcgcga gccagatgca gcgcaacctg atctcgctgc tgcgcgcgag
      601 catgccctcg atgcgctcga ccgactccgg cctgcgcccg ctcgacctgg agctcgcggt
      661 ggtgcgcccg tacctcgaga tcctgaaggt gcgcatggag gaacgcctga ccaccgagat
      721 cgacgtgccc gacggcctgc tgtcggccga gttcccgccg atgatgatcc agacgctggt
      781 cgagaactcg atcaagcacg gcctggaacc caaggccgag ggcgggcacc tgtcggtcaa
      841 ggccgagatc cgccacggca agctggccgt gacggtcgcc gacaccggcc tgggcttcga
      901 ggccgcggcc cagggcacga cccacggcac gggcgtgggc ctgtccaaca tccgcgagcg
      961 cctcgcgctg ctgtacggcg ccaaggcctc gctctcgatc gccaacaacc cggtcggggg
     1021 cacgatcgtg acgatcaccg tcccctacac cgcccacgcg gaatccgccg aaggagcgcc
     1081 agcatgaccg atccgcacct cgacccgacc accggcccgc gcgacgacgg cctcgcctac
     1141 gtgccgcagc cgggcgaccg cccgccgccg tccaccgcgt ccggccgcgc gatgcgcagg
     1201 aagccgagcc tgagcgggcg gatcttccgc tggtggctcg cgatcgcgtt cctgctggcg
     1261 ctcggcgccg ccgcctgcct cgcgttcggc gtcggcgagc tccacggcgg gccgctgcac
     1321 atcaccctgg agggcagcga cctggccgac ggcatcacga tccacggcct gtcggcggac
     1381 ggccaggccg cgctgtcgat ggcggcgctg ctggtggccg tcctgctgct ggtgctgctc
     1441 ccgctgatcc tcgcgctcgt gctggcgctc gtcgcctgcg cgctggtgct ggcgctggcc
     1501 gccccgctgt tcgggctggc ggtcgcgctg gtcgtgctca cgtcgccgct gtggctcgtc
     1561 gccctgtgcg tctggctcgt ggcccgcagc cgtcgtccgc cgccgccgca ctcggctacg
     1621 atcgccgcat gaacgccccg accaccgatc ccaccgcccc ggccgccgtg cgcggcgtca
     1681 tcgccgacga cgagcgcctg atgcgcgacc agctcaaggc gcgcctggcc gaggtctggc
     1741 ccgagctgca gatcgtcggc gaggccaaga acggcctgga ggcggtcgag ctggtcgcgc
     1801 agcaccggcc ggacgtggtc ttcctcgaca tccgcatgcc cggcctgtcg ggcgtcgacg
     1861 cggcccgccg catcgcgcag ctgcccgccc gcgaggacga ccaggacgac gaggacgacc
     1921 acgcgctgcc cgagatcgtc ttcatcaccg cctacgacca gtacgcgatc gaggcgttcg
     1981 agcagggcgt ggccgactat gtcctcaagc ccgccgagcg cgagcgcctg ggcgtgaccg
     2041 tcgcgcgcat caagcagcgc ctggccttgc gcggcaccga ggacgagccg cccgcgccgc
     2101 acctgcagca gctgctccac aagctctcgg ccaagctcaa cccgggcggc acgccgccga
     2161 cgctgaagtg gatccaggcg accgtcggcc aggcgatcca gatgatcccg gtcgaggacg
     2221 tgctgttctt catctccgac gagaagtaca cgcgcgtgca gacggccacg ctcgaggcgc
     2281 tgatccgcaa gccgatcaag gagctcgtcg acgagctcga ccccgacctg ttctggcaga
     2341 tccaccgctc cacgctcgtc aacacgcacg ccatcgccgg cgtgacgcgc gaccttcgcg
     2401 ggcgccagat cgtcagcgtg aaggggcaca acgagaagct cgaggtcagc cgctcgtaca
     2461 cgcacctgtt caaggggatg tgagtcgccg cgccggccgg cgcgggcggt gaaggcggcc
     2521 cgtcaggctt tctgcgccgc gcgggcgatg agccactgcc cgagccagta gatcgacagg
     2581 ttccacaacg tgggcatcgg cacgccgccg acgaacttgt cgatcgcgag gacggcgtcg
     2641 gacgcgacga acagcgcgcc gccgagcgcg accaggcccc acttcgccgc atcgggcgcg
     2701 tccgagcgca cgcgccaggc ggtgctcgcc tgcgccgcca tcgcggacag cgcggtcacg
     2761 tagacgacca ccggcacgcg cagctgcgcc ggcacaccgt gccacaggaa ggacagcacg
     2821 cccgcggcga ccagcgccca ggcgacgaag gcgagcggca ccgcggcgag ccgcacgcgc
     2881 cgcgtcagcg cgacgaggta ggccaggtgc ccgagcagga aggacaccag ccccgcgagg
     2941 aagccctgca ccggccacag cagcgcgacg tcgccggcca gcgaacatgc gagcccgacg
     3001 acgatccagc gcctcacggg cgcgtcgtcc gtctcggcgc tcgcggccca ggcgatcacg
     3061 cacagggtcg cgagcggctt gaacgcgaag ttgagccacg gctgcccgag cgcccacggc
     3121 gcggaagcga tcgccagcgc ggcgcatagc gcgcccagca cgggcagcgc gcgacgccag
     3181 gcgggcggcg aggcggaacc cggagcagga gcaggagcgg gaacggcg
//
LOCUS       DASVLN010000521         1983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_512328, whole genome shotgun sequence.
ACCESSION   DASVLN010000521 DASVLN010000000
VERSION     DASVLN010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512328"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VF453_12730"
     CDS             <1..330
                     /locus_tag="VF453_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688569.1"
                     /translation="GLAGVAGGVVPVYDLGVLAGYPPGDRPSWMAVCAAAPVALAFDA
                     FDGQVSHAGATREAVAAGAVLRIAGEARPLIDVGALIERVRASIPSSTPPSTSSPTAA
                     AAADREN"
     gene            332..1792
                     /locus_tag="VF453_12735"
     CDS             332..1792
                     /locus_tag="VF453_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX7688570.1"
                     /translation="MWTFGRKIAVGFGISFVLLLGIGAVAYRGVDALTKASYSVTHTH
                     QVIEHVVGMLSQLQEAEASQRGFLLTGDESYLDPYNTAVGLVGKLEHELRGLTADNPN
                     QQRRLDEADAVVQARLGELAHNIQLRRDGGLDAGAKAVAAGAGKRTMDTLRSISQQME
                     AEERELLRQRAEEVELTAERARQTVVAGTVLSLLFVCAAGIVIVRSLASQIGSAVRHI
                     QSSSAELHAAAAQQASSAKESSTSMAEITATISELLATSRQIAESAQQVARVAEQTAS
                     AARAGDGTVNAASESIASIRQQVDLVVDHTLELGRKSQQIGAVLDIVSELAEQTNILA
                     INATIEAAGAGESGKRFSVVADEIRKLADRVSGSAKEIRALIDDVRGAVNTTVMATET
                     GAKAVDAGTREFAQVAAVFGQIAGMVVTAMQAAREIELSTKQQATAVEQVNLAVGNSA
                     QAARETEVSLGQTVQTASQLSTLSLGLMRLVQARSA"
BASE COUNT          297 a          708 c          731 g          247 t
ORIGIN      
        1 ggcctggctg gcgtggcggg cggcgtcgtc ccggtgtacg acctcggcgt gctcgcgggc
       61 tatccgcccg gcgaccggcc gtcgtggatg gccgtctgcg cggcggcgcc ggtcgcgctc
      121 gccttcgacg cgttcgacgg ccaggtgagc cacgcgggcg cgacgcgcga ggccgttgcc
      181 gccggtgcgg tcctgcgcat cgcgggcgag gcgcgtccgc tcatcgacgt cggggcgctc
      241 atcgaacgcg tgcgcgcgtc gataccgtcg tcgacgcccc cctcgacatc gtcgccaact
      301 gcggcagcgg ccgcggaccg ggagaactga gatgtggaca ttcggccgca agatcgccgt
      361 cggcttcggg atttcgttcg tgctgctgct gggcatcggc gcggtcgcct accggggcgt
      421 cgacgcgctc acgaaggcga gctactcggt cacccacacg caccaggtga tcgagcacgt
      481 ggtcggcatg ctcagccagc tgcaggaggc cgaggcgagc cagcgcggct tcctgctgac
      541 cggcgacgag agctacctcg atccatacaa cacggccgtg ggcctcgtgg gcaagctgga
      601 gcacgagctg cgcggcctga ccgccgacaa cccgaaccag cagcgccgtc tcgacgaggc
      661 ggacgccgtc gtgcaggcgc ggctcgggga gcttgcgcac aacatccagc tgcgccgcga
      721 cggcggcctg gacgccggcg cgaaggcggt ggccgccggc gccggcaagc gcacgatgga
      781 cacgctgcgt tccatcagcc agcagatgga ggcggaggag cgcgagctgc tgcgccagcg
      841 cgccgaggag gtcgagctca cggccgagcg cgcgcgccag accgtcgtcg ccggcaccgt
      901 gctgagcctg ctgttcgtct gcgccgcggg catcgtgatc gtgcgctcgc tcgcgagcca
      961 gatcggctcg gcggtgcgcc acatccagag ctcgtccgcg gagctgcacg cggccgcggc
     1021 gcagcaggcc agcagtgcca aggagtcgtc gacctcgatg gccgagatca cggccaccat
     1081 cagcgagctg ctcgcgacct cgcgccagat cgccgagagc gcgcagcagg tggcgcgcgt
     1141 cgccgagcag accgcgagcg ccgcgcgcgc gggcgacggc acggtcaacg ccgcgagcga
     1201 gtcgatcgcc agcatccgcc agcaggtcga cctggtcgtc gaccacacgc tggagctcgg
     1261 gcgcaagtcg cagcagatcg gcgcggtgct cgacatcgtc tcggagctgg ccgaacagac
     1321 caacatcctc gcgatcaacg ccaccatcga ggccgccggc gcaggcgagt cgggcaagcg
     1381 cttctccgtc gtcgccgacg agatccgcaa gctcgccgac cgcgtctcgg gctcggccaa
     1441 ggagatccgc gcgctcatcg acgacgtgcg cggcgcggtc aacacgaccg tcatggccac
     1501 ggagacgggc gcgaaggcgg tcgacgccgg cacgcgcgag ttcgcgcagg tcgcggccgt
     1561 cttcggccag atcgccggca tggtcgtcac cgcgatgcag gccgcgcgcg agatcgagct
     1621 gtcgaccaag cagcaggcca ccgccgtgga gcaggtcaac ctggccgtcg gcaacagcgc
     1681 ccaggcggcg cgcgagaccg aggtgagcct cgggcagacc gtgcagacgg cgtcgcagct
     1741 gtccacgctg tcgctcggcc tgatgcgact cgtgcaggcg cggtcggcct gaacgtcgat
     1801 acctgaaacc gtatgccgaa ggacgcctac aagtacttcc gcatcgaggc gcgcgagctg
     1861 ctcgacgagc tcgggcaggg cgtgctcgcg ctggagcgcg gcgcgagcgc gcgcgacggc
     1921 gtgccgccgc tgctgcgcct cgcgcacacg ctcaagggcg cggcgcgcgt cgttcggcag
     1981 gcc
//
LOCUS       DASVLN010000522         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_296819, whole genome shotgun sequence.
ACCESSION   DASVLN010000522 DASVLN010000000
VERSION     DASVLN010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296819"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VF453_12740"
     CDS             <1..861
                     /locus_tag="VF453_12740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7688571.1"
                     /translation="VALKTVRIGPAAGSDRELWRERFMREAAAAAMLRHEHVVLVHAG
                     GVQGEGDALTGWLAMEWVHGTDMSRYATAARLLPEGVVLGVAQRVALALDFAHRAGVV
                     HCDVKPSNVLFDPTTGTVKVTDFGSARVADTVATRSGLIVGTPAYMAPELIAGEPATP
                     QCDLYSLGVTLFELLVGRRPFEASSMGDLLARIAHENPPRLRSLRPELPALLDDVVAH
                     LLAKAPAMRQASAREVAIELRLAQSQCAGALPGTAAAAWADTSPGEDPEAVPPSGRTA
                     NRAFQGTIAQ"
     gene            900..1685
                     /locus_tag="VF453_12745"
     CDS             900..1685
                     /locus_tag="VF453_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX7688572.1"
                     /translation="MILEFFSVTDTGRARRNNEDSVTLDEAGQIAVLADGMGGYNAGE
                     VASGMASERVKGELVARLKPLGPTPSENDIRNALLDSVDAANRAVFDAAMSHAEYAGM
                     GTTLVVAVYRGDRLWLGHIGDSRAYRLRNGHLEQLTRDHSLLQEQIDAGLITAEQAAY
                     SMHKNLVTRAVGVDEVVDLEIHDYPVEAGDLLLMCSDGLSDMLSDEQIEQLLRANDSL
                     PAAGALLVQAANAAGGRDNIAVVLARAKPAPGAFGKSWWSFWR"
     gene            1761..2411
                     /locus_tag="VF453_12750"
     CDS             1761..2411
                     /locus_tag="VF453_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7688573.1"
                     /translation="MGKLVVSLDNVVIKEFQITKERTTLGRRPYNDIVIDNLAVSGEH
                     AVLTASQNDVFIEDLNSTNGTYINGKAVKKQLLSNNDVVEVGKYRIKYLAEDVEDYEK
                     TMILRPGAFGRAPGAAGAMPPPAGPVPMGPASIKVLNGGAAGREVLLTKVVTTVGKPG
                     VQVASVTKRPGGYVLSHVEGQRRPSVNGQAVGDDSVALKDGDIIDLAGTQMQFIQS"
BASE COUNT          385 a          923 c          863 g          344 t
ORIGIN      
        1 gtcgccctca agacggtgcg catcgggccg gcggcgggca gcgaccggga gctctggcgc
       61 gagcgcttca tgcgcgaagc ggccgccgcc gcgatgctgc gccacgagca cgtcgtgctc
      121 gtgcacgcgg gcggcgtgca gggcgaaggc gacgcgctga cgggctggct cgcgatggag
      181 tgggtgcacg gcaccgacat gtcgcgctac gccacggctg cgcgcctgct gcccgagggc
      241 gtggtcctgg gcgtcgcgca gcgcgtggcg ctggcgctcg acttcgcgca ccgcgccggc
      301 gtcgtccatt gtgacgtcaa gccgtccaac gtgctgttcg acccgaccac cggcaccgtc
      361 aaggtgaccg acttcggttc cgcccgcgtc gccgacacgg tcgccacgcg cagcgggctg
      421 atcgtcggca cgccggccta catggcgccc gagctgatcg ccggcgagcc ggcgacgccg
      481 cagtgcgacc tctacagcct gggcgtgacg ctgttcgagc tgctggtcgg ccgccgcccg
      541 ttcgaggcga gctcgatggg cgacctgctc gcccggatcg cgcacgagaa cccgccccgc
      601 ctgcgcagcc tgcgtcccga gttgccggcg ctgctggacg acgtcgtcgc ccacctgctc
      661 gccaaggcgc cggccatgcg ccaggcgagc gcgcgcgagg tcgcgatcga gctgcggctc
      721 gcgcagtccc agtgcgccgg ggcgctgccc ggcaccgccg cggcggcctg ggcggacacc
      781 tcccccggtg aagaccccga ggccgttccc ccttccgggc ggacggcgaa ccgggcattc
      841 caaggcacaa ttgcacaata accgcgcgat ttccgcgatc gctgcatccc gtcgccctca
      901 tgatccttga gttcttcagc gtcaccgaca ccggccgcgc gcgccgcaac aacgaagact
      961 ccgtgacgct cgacgaagcc gggcagatcg cggtgttggc ggacggcatg ggcggctaca
     1021 acgcgggcga ggtcgcgagc ggcatggcgt ccgagcgcgt gaagggcgag ctcgtcgccc
     1081 ggctgaagcc cctcggcccg acgccgtcgg agaacgacat ccgcaacgcg ctgctcgact
     1141 cggtcgacgc cgccaaccgc gccgtcttcg acgccgcgat gagccacgcg gagtacgccg
     1201 gcatgggcac gaccctggtc gtggccgtct accgcggcga ccggctctgg ctcggccaca
     1261 tcggcgactc gcgcgcctac cgcctgcgca acggccacct cgagcaactc acgcgcgacc
     1321 actcgctgct gcaggagcag atcgacgccg gcctgatcac ggcggagcag gccgcctact
     1381 cgatgcacaa gaacctggtc acgcgggccg tgggcgtgga cgaggtcgtc gacctcgaga
     1441 tccacgacta tcccgtcgag gcaggagact tgcttctgat gtgttcggac ggcctgtccg
     1501 acatgctcag cgacgagcag atcgagcagc tgttgcgagc gaacgacagc ctcccggcgg
     1561 ccggcgccct gctcgtgcag gccgccaacg cggccggcgg ccgggataac atcgcggtgg
     1621 tcctggcgcg tgccaagccc gcgccgggcg cgttcggcaa gtcctggtgg tctttctggc
     1681 gatgaagttc ctatgagtgg catggacgct tcgacgtctc cgacaagcgg cccggaagcc
     1741 gcctgaacaa gaggttttca atgggcaaac tcgtcgtctc gctcgataac gtcgtgatca
     1801 aggagttcca gatcacgaag gagcgcacca cgctcggccg gcggccgtac aacgacatcg
     1861 tcatcgacaa cctcgccgtc agcggcgagc acgcggtgct gacggcgtcg cagaacgacg
     1921 tcttcatcga ggacctcaac agcaccaacg gcacctacat caacggcaag gccgtgaaga
     1981 agcagctgct gtcgaacaac gacgtcgtcg aggtcggcaa gtaccgcatc aagtacctcg
     2041 ccgaggacgt cgaggactac gagaagacga tgatcctgcg tcccggcgcc ttcggccgcg
     2101 cgcccggcgc ggccggcgcc atgcccccgc cggccggccc ggtgccgatg ggcccggcgt
     2161 cgatcaaggt cctcaacggc ggcgccgcgg gccgcgaggt gctgctcacc aaggtcgtga
     2221 cgaccgtcgg caagccgggc gtccaggtcg cctcggtcac gaagcgcccg ggcggctacg
     2281 tgctgtccca tgtcgagggt cagcgccgcc cgagcgtcaa cggacaggcc gtcggcgacg
     2341 actcggtcgc gctgaaggac ggcgacatca tcgacctggc cggcacgcag atgcagttca
     2401 tccagagctg acgttccgcc cccgtcctcg tcccgcggcg tgcccccccc gtccgcccgg
     2461 gcctcgctgc ccgccgccct gcgccgcgcc ggcgccgcct ggcacgggtt gcgcc
//
LOCUS       DASVLN010000523         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_512633, whole genome shotgun sequence.
ACCESSION   DASVLN010000523 DASVLN010000000
VERSION     DASVLN010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512633"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..415
                     /locus_tag="VF453_12755"
     CDS             98..415
                     /locus_tag="VF453_12755"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX7688574.1"
                     /translation="MAGGLSPGELIVDGPDLELNPGRRRATLVVENAGDRPIQVGSHY
                     HFAETNAALRFDRAAARGMRLDIASGTAVRFEPGQQRTVTLVELAGARRVFGFRALVQ
                     GPL"
     gene            417..2144
                     /gene="ureC"
                     /locus_tag="VF453_12760"
     CDS             417..2144
                     /gene="ureC"
                     /locus_tag="VF453_12760"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX7688575.1"
                     /translation="MPTIGRRAYAEMFGPTTGDRVRLADTNLVIEVERDHTLAAGGYG
                     EEVKFGGGKTIRDGMGQGQAVNGPGAADAVDCVITNALIVDHWGIVKADIGLRGCRIA
                     AIGKAGNPDVQPGVDIVIGPGTEIIAAEGLIVTAGAIDTHIHFICPQQIEEALASGVT
                     TMTGGGTGPATGTFATTCTPGPANIERMLQAADAFPMNLGFLGKGNASRPDALRQQVE
                     AGAIGLKLHEDWGTTPAAIDCCLAVAEETDVQVAIHTDTLNESGFVEDTIAAFKGRTI
                     HSFHTEGAGGGHAPDIMKVVGEANVLPSSTNPTRPFTVNTLDEHLDMLMVCHHLDAGI
                     AEDLAFAESRIRRETIAAEDILHDLGAISMFSSDSQAMGRVGEVVLRCWQTAHKMKAQ
                     RGALPGDGDRNDNARVKRYIAKLTINPALTHGIAHEVGGVEVGKWADLVLWHPAFFGV
                     KPALVLKGGFIAMAAMGDPNASIPTPQPVHMRPMFGSFAGARTRGSLTFLSQAAIAAG
                     VPARAGLAKPVSAVRGCRTVTKRDMVHNDWLPRMEIDAQTYEVRADGQLLACEPATEL
                     PMAQRYFLF"
     gene            2154..2711
                     /gene="ureE"
                     /locus_tag="VF453_12765"
                     /pseudo
     CDS             2154..2711
                     /gene="ureE"
                     /locus_tag="VF453_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
     gene            2718..>2840
                     /locus_tag="VF453_12770"
     CDS             2718..>2840
                     /locus_tag="VF453_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HEX7688576.1"
                     /translation="MRDATLPDATTLLQLIWLASPALPVGGFSYSEALEAAVDDG"
BASE COUNT          411 a         1062 c         1009 g          358 t
ORIGIN      
        1 tcggcgtcgc gctgcgcggc cgcagcgcct ggtggccgcg cgccgcaggc gcgggcctcg
       61 cggccttcgg gctcgcgctg ctgggagcgg ccgcctgatg gccggcggcc tctcgcccgg
      121 cgagttgatc gtcgacggcc cggatctcga gctcaacccg ggccgccgtc gcgcgacgct
      181 ggtcgtcgag aacgccggcg accgcccgat ccaggtcggc tcgcactacc atttcgcgga
      241 gacgaacgcc gcgctgcgct tcgaccgcgc cgccgcgcgc ggcatgcggc tggacatcgc
      301 ctccggcacc gccgtgcgct tcgagcccgg ccagcagcgc accgtgacgc tcgtcgagct
      361 cgccggcgcg cgcagggtct tcggcttccg cgccctcgtc caaggaccgc tctgacatgc
      421 ccaccatcgg tcgccgcgcc tacgcggaga tgttcggccc cacgaccggc gaccgcgtgc
      481 ggctcgcgga cacgaacctc gtgatcgagg tcgagcgcga ccacaccctg gccgcgggcg
      541 gctacggcga ggaggtcaag ttcggcggcg gcaagacgat ccgcgacggc atgggccagg
      601 gccaggcggt caacggaccg ggcgcggccg acgccgtcga ctgcgtgatc acgaacgcgc
      661 tgatcgtcga ccactggggc atcgtcaagg ccgacatcgg cctgcgcggc tgccgcatcg
      721 cggccatcgg caaggccggc aacccggacg tgcaacccgg cgtggacatc gtcatcgggc
      781 cgggcaccga gatcatcgcg gccgagggcc tgatcgtcac cgcgggggcg atcgacaccc
      841 acatccactt catctgcccg cagcagatcg aggaggcgct ggccagcggc gtgacgacga
      901 tgaccggcgg cggcaccggc cccgcgaccg gcaccttcgc gaccacctgc acgcccggcc
      961 ccgccaacat cgagcgcatg ctgcaggcgg cggatgcatt cccgatgaac ctcggcttcc
     1021 tcggcaaggg caacgcgagc cggcccgacg ccctgcgcca gcaggtcgag gcgggtgcga
     1081 tcggcctgaa gctgcacgag gactggggca cgacgcctgc ggcgatcgac tgctgcctgg
     1141 cggtggccga ggagaccgac gtccaggtcg cgatccacac ggacacgctc aacgagagcg
     1201 gcttcgtcga ggacacgatc gcggcgttca agggccgcac gatccacagc ttccacaccg
     1261 agggcgccgg cggcggccac gcgcccgaca tcatgaaggt ggtcggcgag gccaacgtgc
     1321 tgccctcgtc gaccaacccg acgcgcccgt tcacggtcaa cacgctggac gagcacctcg
     1381 acatgctgat ggtgtgccac cacctggacg ccggcatcgc ggaggatctc gcgttcgccg
     1441 agagccgcat ccgccgcgag accatcgccg ccgaggacat cctgcacgac ctcggcgcga
     1501 tcagcatgtt ctcgtcggac tcgcaggcca tgggccgcgt cggcgaggtc gtgctgcgct
     1561 gctggcagac ggcacacaag atgaaggccc agcgcggcgc gctgcccggc gacggcgacc
     1621 gcaacgacaa cgcgcgcgtg aagcgctaca tcgccaagct caccatcaac cccgctctga
     1681 cgcatggcat cgcgcacgag gtcggcgggg tcgaggtcgg caagtgggcc gacctggtgc
     1741 tgtggcatcc ggccttcttc ggcgtcaagc cggcgctcgt gctcaagggc ggcttcatcg
     1801 cgatggccgc gatgggcgac ccgaacgcgt ccatccccac gccgcagccg gtgcacatgc
     1861 ggccgatgtt cggcagcttc gcgggcgcgc gtacccgcgg ctcgctgacc ttcctgtcgc
     1921 aggccgcgat agccgccggc gtgccggcgc gcgcggggct cgcgaagccg gtctccgccg
     1981 tgcgcggctg ccgcaccgtc accaagcgcg acatggtgca caacgactgg ctgccgcgca
     2041 tggagatcga cgcccagacc tacgaggtgc gcgccgacgg ccagctgctc gcgtgcgagc
     2101 ccgcgaccga gctgccgatg gcgcagcgct atttcctgtt ctgagggcag gcgatgagcg
     2161 acgacaggcc ggcggccctc gaggtcggca agcgcatccc cggcggccgg ggattgagcg
     2221 gcgcgctcgt ccggcgcgcg agcgaggtcg agctggactg ggacctccgg cagcgcagcc
     2281 gcatcgaggc caccgactcg cacggccgcg cgctgcacgt gttcctgccc cgcggcaccg
     2341 tgctgcgcgg gggcgacgtg ctcgtggccg aggacggctc gctgatccgc gtgaaggccg
     2401 cgccgcagcc ggtgctggtg gtcacgcact gccccgcgca cggctcgccc ttcgacctgc
     2461 tgcgcgcggc ctaccacctc ggcaaccgcc acgtgccgct ggagctgcgg cccgaccgcc
     2521 tgctgctgga gcccgaccac gtgctggccg acatgctgcg ccgccagggg cttctcgtcg
     2581 ccgaggccgc gagcgccttc gagccggagg cgggcgccta cgtcgccggg ggcgggcatg
     2641 cccacggcca cggccattcg catggcgatc cgcatggcca cgcgcacggc cacggacacc
     2701 atcacggaca ttgaggcatg cgcgacgcga ccctgcccga cgcgacgacg ctgctgcagc
     2761 tgatctggct cgcgtcgccg gcgctgccgg tcggcggctt cagctattcg gaagcgctgg
     2821 aggcggccgt cgacgacggg
//
LOCUS       DASVLN010000524         3423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_188425, whole genome shotgun sequence.
ACCESSION   DASVLN010000524 DASVLN010000000
VERSION     DASVLN010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188425"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2757)
                     /locus_tag="VF453_12775"
     CDS             complement(<1..2757)
                     /locus_tag="VF453_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7688577.1"
                     /translation="MSERELRASAEDAARSLVENAAAGMVVVQDGRIAYANPCAARLL
                     GRAVDVLERMSPGELLQGDDRTRQMEATAALLARRSDEEYNEYRIELPDGRARQFAVH
                     STRTDWRGRPATLGVLHDISRQRAATAALRGSEERYRSMVESLSEGVLVFDAAGRIKA
                     ANGAAERLLGMPFERLRRNQIEDWRPLDLDGTAMPPERIPVARVLATGEAHPHTVLGM
                     QHPDGRTLWLDTNAVPLRDPASQVLTGALITLTDVTEARRTEEALRISESTNRALMDA
                     LADGVFVAQDYRFAYANRLLLDALGYTREEFIGMPFERVIAPAWLDTWMERYTRRVGA
                     GPEPVHTYEVGFLRKDGTIADFELVANRTTYLNAPGVLGVLRDISERKAAAAELEQHR
                     HRLEELVAQRTRALEEAVAGRVASENFAQTMTDHQPNLLAYVDNRLCVQFANQAYLAF
                     FGKSREEVIGRGVDDTLAQALVHPPHTVIERVLGGEAVGISTDMRGADGQVRSFWIHR
                     SPDVVDGESRGYFFIATDTTEMRRSERRLQELNAKLIQAESFTRGIADNLPVRIAYWD
                     ADQRCRFVNRAYCEWAGLSVNQMLGRTWSEIFGEQHGAERRAHMDEALQGRLQEFESV
                     EAGADGRVGVWNKQYIPDMPRGAVRGFFVLAQEITNAKRAQADLESLNQELVHARDAA
                     EEAARAKSAFLANMSHEIRTPMNVIIGLTHLMRRDAHDPVTTDRLAKVGDAAQHLLDI
                     INDILDLSKIDAGKLALAPVDFAVEGLLERSTAMVADGARRKGLSLSVDRGDLPDALH
                     GDALRLSQVLVNLLSNAVKFTERGSIALRCAVLARDPGGVTVRFSVHDTGLGISEEQR
                     ERLFRPFEQADGSTTRRFGGTGLGLSIARELAQLMGGAIGVESEPGRGSVFWFTARLQ
                     PALAA"
     gene            complement(2923..>3423)
                     /locus_tag="VF453_12780"
     CDS             complement(2923..>3423)
                     /locus_tag="VF453_12780"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX7688578.1"
                     /translation="GFFPGSTIGNFTPDAAADLLGRLAQALGPRSRLLIGVDTTQDPA
                     LLLPAYDDAAGVTAAFNLNLLDRINRELDGDFDRTAFRHLARHDPVEGRIEMHLVSLR
                     DQQVRVRGRAFWFEAGETIHTENCYKYTPGRFLHLAHAANWRCERAWQDGGRTGFTVY
                     LMQRDA"
BASE COUNT          453 a         1218 c         1234 g          518 t
ORIGIN      
        1 ggcggcgagc gcgggctgca gccgcgccgt gaaccagaag acgctgccgc ggccgggctc
       61 gctctcgacg ccgatcgcgc cgcccatcaa ctgcgccagc tcgcgcgcga tcgacagccc
      121 gagcccggtg ccgccgaagc gccgcgtcgt cgagccgtcg gcctgctcga acgggcggaa
      181 caggcgctcg cgctgctcct ccgatatacc caagcctgta tcgtgcacgg aaaagcgcac
      241 cgtcaccccg cccgggtcgc gcgcgagcac cgcgcagcgc agcgcgatgc tgccgcgctc
      301 ggtgaacttg acggcgttgc tcagcaggtt gaccagcacc tgcgacaggc gcagcgcatc
      361 gccgtggagc gcgtcgggca gatcgccgcg gtcgacgctc agcgaaagcc cctttcggcg
      421 cgcaccgtcg gccaccatgg ccgtgctgcg ctcgagcagc ccctcgaccg cgaaatcgac
      481 cggcgccagc gcgagcttgc ccgcgtcgat cttggacagg tcgaggatgt cgttgatgat
      541 gtccagcagg tgctgcgcgg cgtcgcccac cttggcgagg cggtcggtgg tcaccgggtc
      601 gtgcgcatcg cgccgcatca ggtgcgtgag cccgatgatc acgttcatcg gcgtgcggat
      661 ctcgtggctc atgttggcga ggaaggccga cttcgcgcgc gccgcctcct ccgcggcgtc
      721 gcgcgcgtgg acgagttcct ggttgagcga ctcgaggtcg gcctgcgcgc gcttggcgtt
      781 ggtgatctcc tgcgccagca cgaagaagcc gcgcacggcg ccgcgcggca tgtccgggat
      841 gtactgcttg ttccacacgc ccacgcgccc gtcggcgccg gcctccacgc tctcgaactc
      901 ctgcagccgg ccctgcagcg cctcgtccat gtgcgcccgc cgctcggcgc cgtgctgctc
      961 gccgaagatc tcgctccagg tgcggcccag catctggttg accgacagcc ccgcccattc
     1021 gcagtaggcg cgattgacga agcgacagcg ctggtcggcg tcccagtagg cgatgcgcac
     1081 cggcaggttg tcggcgatgc cgcgcgtgaa ggattcggcc tggatcagct tggcgttgag
     1141 ctcctgcagc cggcgctcgc tgcgccgcat ctccgtcgtg tcggtcgcga tgaagaagta
     1201 gccccggctc tcgccgtcga cgacgtcggg gctgcggtgg atccagaagc tgcgcacctg
     1261 gccgtcggcg ccgcgcatgt cggtcgagat gcccaccgcc tcgccaccca gcacgcgctc
     1321 gatcaccgtg tgcggcgggt gcaccagcgc ctgggcgagc gtgtcgtcca cgccgcggcc
     1381 gatcacctct tcgcgcgact tgccgaagaa ggcgaggtag gcctggttgg cgaactgcac
     1441 gcacagccgg ttgtcgacgt aggccagcag gttgggctgg tggtcggtca tcgtctgcgc
     1501 gaagttctcg ctcgcgacgc ggccggcgac ggcctcctcg agcgcgcgcg tgcgctgcgc
     1561 gacgagctcc tcgagccggt ggcggtgctg ctcgagctcc gcggccgcgg ccttgcgctc
     1621 gctgatgtcg cgcagcacgc ccagcacgcc gggcgcattg aggtaggtcg tgcgattggc
     1681 gaccagttcg aagtcggcga tggtgccgtc cttgcgcagg aagccgacct cgtacgtgtg
     1741 caccggctcc ggccccgcgc cgacgcgccg ggtgtagcgc tccatccagg tgtccagcca
     1801 cgccggcgcg atcacgcgct cgaaaggcat gccgatgaac tcctcgcgcg tgtagccgag
     1861 cgcgtccagc agcagccggt tggcgtaggc gaagcggtag tcctgcgcca cgaacacgcc
     1921 gtcggccagc gcgtccatca gcgcgcggtt ggtgctctcg ctgatgcgca gggcctcctc
     1981 ggtgcgccgc gcctcggtga cgtccgtcaa ggtgatcagc gcgccggtca gcacctgcga
     2041 ggccgggtcg cgcagcggga cggcgttggt gtccagccac agcgtccggc cgtcgggatg
     2101 ctgcatacca agtaccgtat gcgggtgggc ctcgcccgtc gccagcaccc gggcgacggg
     2161 aatgcgctcc ggcggcatcg ccgtgccgtc caggtcgagc ggccgccagt cctcgatctg
     2221 gttgcgacgc aggcgctcga acggcatgcc cagcaggcgc tccgcggcgc cgttcgcggc
     2281 cttgatgcgg ccggcggcgt cgaacaccag cacgccttcg ctcagcgact cgaccatcga
     2341 gcggtagcgc tcctcgctgc cgcgcaacgc ggcggtcgcg gcgcgctggc gcgagatgtc
     2401 gtgcagcacg cccagcgtgg cggggcggcc gcgccagtcc gtgcgcgtcg agtgcaccgc
     2461 gaactggcgc gctcgtccgt cgggcagctc gatgcggtac tcgttgtatt cctcgtcgct
     2521 gcgacgcgcg agcagcgcgg ccgtcgcctc catctgccgc gtgcggtcgt cgccctgcag
     2581 cagctcgccg ggcgacatgc gctcgagcac gtcgaccgcg cgcccgagca gccgggccgc
     2641 gcaggggttg gcgtaggcga tgcggccgtc ctgcacgacg accatgccgg cggcggcgtt
     2701 ctccaccaga ctgcgtgcgg catcctcggc ggacgcacgc aattcccgtt cgctcatgtc
     2761 aagcgctccc tgggccgatc ctcgtccagg catcggctcg gcggcagatc cttggccagg
     2821 atggcgatcg ggggagttca cctcggcaag ggacgcctgt caagccatgg acagccccgc
     2881 accgggccgg gcaagcgggc cgcctgatcg tccggttagg cctcaggcgt cgcgctgcat
     2941 gaggtacacc gtgaagccgg tgcggccacc gtcctgccac gcgcgctcgc agcgccagtt
     3001 cgccgcatgg gccaggtgca ggaagcgccc gggcgtgtac ttgtagcagt tctcggtgtg
     3061 gatggtctcg ccggcctcga accagaacgc gcggccgcgc acgcgcacct gctggtcgcg
     3121 caggctgacg aggtgcatct cgatgcggcc ttccacgggg tcgtggcgcg ccaggtggcg
     3181 gaacgccgtg cggtcgaagt cgccgtcgag ctcgcggttg atgcggtcca ggaggttgag
     3241 gttgaacgcg gcggtcacgc cggcggcgtc gtcgtaggcc ggcagcagca gcgccggatc
     3301 ctgcgtcgtg tcgacgccga tgagcagccg cgagcgcggc cccagggcct gcgcgaggcg
     3361 gcccagcagg tcggcggccg cgtcgggcgt gaagttgccg atggtcgagc cggggaagaa
     3421 gcc
//
LOCUS       DASVLN010000525         2390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_349198, whole genome shotgun sequence.
ACCESSION   DASVLN010000525 DASVLN010000000
VERSION     DASVLN010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349198"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF453_12785"
     CDS             complement(<1..376)
                     /locus_tag="VF453_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688579.1"
                     /translation="MGISAIRSQPGTTNADMFGLREQASGNGFQGLAQQGGAQGLSTL
                     HHHHHHHEVSADGADGADGSDPMKNPDIQQLLQLILQTLQQLFAQNDDSGDGDDGCVG
                     SAQPQSGGAQPGVGFGGGGGGGG"
     gene            complement(563..1441)
                     /locus_tag="VF453_12790"
     CDS             complement(563..1441)
                     /locus_tag="VF453_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688580.1"
                     /translation="MDLNALADFHLVAGHGGFGRASRAGRRSKATLSRRIAELEEALG
                     VRLIERNAKGLELTEAGQQLLSRTVGPLHEVTDAIASTREGTGKVAGRLRIAAPILFS
                     QLAMGRLCAEFRARHPDVLVEVAGEDRLVDLVDEQFDVAIRPNPRPDTALVGRRFAKD
                     RLVVVAAPSIAVPAAAGTPVRVPAAVNALRDGDTWTLADTGLVVEPVPVLRLSSMLML
                     RDAALAGGGVALVPQSIVWKQLAAGELVLWGALASTDVELWVLHTSRRLASPKVRAFV
                     DFMASRYPDGLLRLDG"
     gene            1593..>2390
                     /locus_tag="VF453_12795"
     CDS             1593..>2390
                     /locus_tag="VF453_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEX7688581.1"
                     /translation="MSILVIGSTGTIGSLVVQGLVRQGAEVKAFVRQAGKAPAGVREA
                     VGDLADVASVRQAMAGVRTLFLLNALAPDELTQSLQALNVAREAGVQRIVYLSVINAD
                     KYVDVPHFTGKYTVERMIDALDLPATILRPAYFMQNDIQIQPVIENHGVFPMPIGDKG
                     AAMVDARDIADVAVAELLRRDRAAEPLPRLTLDVAGAEVFTGASAAAVWSRALGRTVA
                     YPGDDVAGFEAQLLQNHAPAWMAYDLRLMMSGIQRHGQLPEAGTVARL"
BASE COUNT          346 a          880 c          817 g          347 t
ORIGIN      
        1 caccgccgcc accaccgccg ccgccgaagc cgacgcccgg ctgcgcgccg cccgactgcg
       61 gctgggcgct gccgacgcag ccgtcgtccc catcgccgct gtcgtcgttc tgggcgaaca
      121 actgctgcag cgtctgcagg atcagctgca gcagttgctg gatgtccggg ttcttcatcg
      181 ggtcgctgcc gtcggcgccg tcggcgccgt cggcggagac ctcgtggtga tggtgatggt
      241 ggtgcagggt cgacagcccc tgcgcgccgc cttgctgggc cagcccctgg aagccgttgc
      301 ccgacgcttg ttcccggagg ccgaacatgt cggcgttcgt cgtgccgggt tgagatcgaa
      361 tcgcggaaat acccatcgca gtcctccttg gatgcgcaat gaagggatcg tggtcgcaac
      421 acccacgcgt gcgattggca tcgtggacgc gcgctccacg ccgaccgcgg cccgggcccg
      481 aagcgatgtc gccgcaagcg aagcgcgcgt ctgcccgggc gtcgcggccg ccggtgcgcc
      541 ttgcatcgat ccggcggcgc cgctatccgt ccagccgcag caggccgtcc gggtagcgcg
      601 acgccatgaa gtcgacgaag gcgcggacct tggggctggc caggcgccgg gaggtgtgga
      661 gcacccagag ctccacgtcc gtcgaagcga gcgctcccca caggacgagc tcgcccgcgg
      721 cgagctgctt ccagacgatg gactgcggaa ccagggccac gcccccgccg gcgagcgccg
      781 cgtcccgcag catcagcatc gaggacagcc gcagcacggg caccggctcc acgacgaggc
      841 cggtgtcggc gagcgtccag gtgtctccgt cgcgcaaggc attgaccgcc gctggcaccc
      901 gcaccggcgt ccccgcggcc gcaggcaccg cgatggacgg cgcggccacg accaccagcc
      961 ggtccttcgc gaaccggcgc cccaccaggg ccgtgtcggg gcgcgggttc ggccggatcg
     1021 cgacgtcgaa ctgctcgtcg acgagatcca cgagccgatc ctcccccgcg acctcgacga
     1081 ggacgtccgg atgccgcgcg cggaactcgg cgcacaggcg gcccatggcg agctgggaga
     1141 acaggatggg cgcggcgatc cgcagccgcc cggccacctt gccggtgccc tcccgggtcg
     1201 aggcgatggc gtccgtgacc tcgtgcaggg gcccgaccgt ccggctcagc agctgctgcc
     1261 cggcctccgt gagttccagc cccttggcat tgcgctcgat cagccgcacg cccagcgctt
     1321 cctcgagctc ggcgatccgc cgcgagagcg tggccttgga gcgcctgccg gcgcgactcg
     1381 ctcggccgaa gccgccgtgg ccggcgacca ggtggaagtc tgccagggcg ttgaggtcca
     1441 tgttccaaat ttaagacgga gtgttgagat tttagtgtct gtgttcgatg gatgcaacga
     1501 cgtatcgttc atccatcgcc acccaccccg ggacttcttc tcgaccggcg ggcaggcgat
     1561 cccatccatc aacccacttg cggagtttcg acatgagcat cctcgtcatc ggttccaccg
     1621 gaaccatcgg ttccctcgtc gtccagggcc tcgtccggca gggcgcggag gtcaaggcct
     1681 tcgtgcgcca ggcgggcaag gcgccggccg gcgtccgcga agccgtcggc gacctggccg
     1741 acgtcgcatc ggtgcgccag gcgatggccg gcgttcgcac cctgttcctg ctgaacgccc
     1801 tcgcgcccga cgagctcacg cagtcgctcc aggccctcaa cgtggcgcgc gaggcgggcg
     1861 tgcagcgcat cgtctacctg tcggtgatca acgcggacaa gtacgtcgac gtcccgcact
     1921 tcacgggcaa gtacacggtg gagcgcatga tcgacgccct ggacctgccg gccaccatcc
     1981 tgcgcccggc ctacttcatg cagaacgaca tccagatcca gcccgtcatc gagaaccacg
     2041 gcgtgttccc gatgcccatc ggcgacaagg gcgccgccat ggtggatgcg cgcgacatcg
     2101 ccgacgtcgc ggtcgcggaa ctgctgcggc gcgaccgcgc cgccgagccg ctgccgcgcc
     2161 tgacgctgga cgtcgccggc gcggaggtct tcaccggcgc ctccgcggcg gcggtctgga
     2221 gccgcgccct gggccgcacc gtcgcctacc cgggcgacga cgtcgcgggc ttcgaggcgc
     2281 aactgctgca gaaccatgcc cccgcctgga tggcctacga cctgcgcctg atgatgagcg
     2341 gcatccagcg tcacggccag ttgccggagg ccggcacggt ggccaggctc
//
LOCUS       DASVLN010000526         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_241805, whole genome shotgun sequence.
ACCESSION   DASVLN010000526 DASVLN010000000
VERSION     DASVLN010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241805"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1016..1333)
                     /locus_tag="VF453_12800"
     CDS             complement(1016..1333)
                     /locus_tag="VF453_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688582.1"
                     /translation="MAVVVRWAGAMVVGHAARAGGGSRAVIAVVPAGIAERPSGCDRV
                     AGNGAKAAIAAVDGDVVLRLRVRSSADVRGGRTGCPGIGVIARLEGPGPAPMRSQRRW
                     TGP"
     gene            1349..2101
                     /locus_tag="VF453_12805"
     CDS             1349..2101
                     /locus_tag="VF453_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688583.1"
                     /translation="MRAEGGDALLAGLGRRFRGATIQIEFELRTTAVRQVFRRDFVYV
                     SQQAHALESSRRVQGIDRSRLTEALRLVERRGESIRATLEDAIVQARELVRRAGELPQ
                     DIRFTRAAGLQATIVSPYARLFVELMRLADEAISLLEGAWLMGALDSAERAELINECR
                     RVLVSYKDVVRDQRHAIGEHVRAVNEERRRTRGHDGLRRHDPASPGHAPMSRRHGTRP
                     VTESGPVPGPGPEAGIGGAGLRTDDEGFADAP"
     gene            complement(2333..>2527)
                     /locus_tag="VF453_12810"
     CDS             complement(2333..>2527)
                     /locus_tag="VF453_12810"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX7688584.1"
                     /translation="APAPVVAPPVVAPAAVAPAHAASPAPAATPDAEQRLTTLKRLYE
                     KGLITKSEYEKKRQEILKSL"
BASE COUNT          360 a          905 c          938 g          324 t
ORIGIN      
        1 cggcgccccg gcccgggccg tgtccatgac gggcgcgccg tcgggaaggc tcgcgtgcaa
       61 ccgcggcggg tgctcgacgc aaccgggctc gatcgattcg gcagggacgc gggtatcggg
      121 ctgcagggcg agctccgggg gtggacggga gtcgggcgga atcgcggcct cgcgtggtcg
      181 cgagcccggt gtccgcccgg gggtgggtcg aatcgatcat aagagcgggc gtccggaagc
      241 gcccgcggcg aaacgcccgg aaatgagccg aaatgacgcg ggcggaagcg gccctcgtgg
      301 cgggtgtcgc cgtcgccgcg gctggcgagt cccgtcgccc gcgtgcggtc acagcagtcg
      361 ttttcggtca ccggccgtgg cccgacatcg cagcgacaac gttttcctag cttccatgcg
      421 gcatggcatc gccgcgcagc gcgcgaaacc ggccatttat cggcctgttt gacgtccggg
      481 gtttcgcggc caccggctta acgtgagtgc acacatggga gaaacgaccg tgaatgccaa
      541 ccacgacgac gacgccttcg atgcgtcgca cccgctgccg cccggcggcc tcaccgattc
      601 catcgacgcc gcgctcacgc aggccgcgtc caacgagcag gcatccgacg caccggattc
      661 caaccctctg gttccccacg atccggagtc cggagggcgc ggtgcggccg cctcgcaacc
      721 tgggaaccac gagccttcct ggcatgcgcg cctgcaagcg agcgctgccg cgcccggcgg
      781 ggaatcgcct ccacccacgg cgctcgcatc tgcactgatg gcggccttgt cgggtacgcc
      841 gggcgcccgg gcgccgacct cccccgcgtc accggtgcga gagcctccgg acagcgagac
      901 cctggcgcgc gccgcggccg cggccgacat cgtgatgcgc cgcgccggcg gcatggcggc
      961 cggagaggcg ccggcaacgg gtgccgccaa cgcgcagccc gcacggcggc cgatgctacg
     1021 ggccagtcca tcttcgctgc gagcgcatgg gagccgggcc cggtccttcg agccgcgcga
     1081 tcacaccgat gccggggcac ccggttcggc cgcctcggac gtcggcgctg gagcggaccc
     1141 gcagccggag caccacatcg ccatcgacgg cggcgatcgc ggccttcgcc ccgttgccgg
     1201 cgacccggtc gcagccggat ggacgctcgg caatgccggc cgggacgacg gcgatgacag
     1261 cccgcgaacc gccgcccgcg cgggcggcgt ggccgacgac catggcgccc gcccatcgga
     1321 cgacgaccgc catgcgcgct ccacgccgtt gcgtgcggag ggtggcgacg cgctgcttgc
     1381 cggactgggt cgccgcttcc gcggggccac gatccagatc gagttcgagt tgaggacgac
     1441 ggccgtgcgc caggtcttcc ggcgggactt cgtctacgtc tcgcagcagg cgcatgccct
     1501 ggagtccagc cggcgcgtgc agggcatcga ccggtcgcga ttgacggagg cactgcgcct
     1561 cgtggagcgc aggggcgagt ccatccgcgc cacgctcgag gacgcgatcg tgcaggcacg
     1621 cgagctcgtc cgccgcgcgg gcgagttgcc gcaggacatc cgcttcacgc gcgcggccgg
     1681 cctgcaggcg accatcgtga gcccgtacgc gcggctgttc gtcgagctga tgcgcctcgc
     1741 cgacgaggcg atctcgctgc tggaaggagc gtggctgatg ggcgccctgg actccgcgga
     1801 acgcgccgag ctgatcaacg aatgccgccg cgtcctcgtg agctacaagg acgtcgtccg
     1861 cgaccagcgt catgcgatcg gcgagcatgt ccgcgcggtc aacgaggagc gacgacggac
     1921 tcgcgggcac gacggcctgc ggcgacacga tccggcatcc ccggggcacg cgccgatgtc
     1981 gcgccgccat gggacgcgtc cggtgacgga atcgggcccc gtgcccggac cgggaccgga
     2041 ggcggggatc ggcggcgccg gactccggac ggacgacgag ggcttcgcgg atgcaccgtg
     2101 aggagtcatc cggcgcgccg gcgagcgcac ggttgcgcga gccaccgact ccggcacggg
     2161 caagggagcc gacgtcgaca ggtttcccgc ggccgatcgc aggcgaatgc ggactcgcaa
     2221 cgccaggaca ggcgcgcgca atcctgccca agcatgcacg gccgcgaatg cgcgcgcatg
     2281 cacgcggctg cgcggatgct tgcgaacgcg ctcacctctc cggcggcgcg cgtcagaggc
     2341 tcttgaggat ctcctggcgc ttcttttcgt actcggactt ggtgatcagg cccttctcgt
     2401 acaggcgctt gagtgtcgtc aggcgctgtt cggcgtcggg cgtcgcagcc ggcgcggggg
     2461 acgccgcgtg ggccggcgcg acggcagcag gcgcgacgac gggcggagcg accaccggcg
     2521 ccggcgc
//
LOCUS       DASVLN010000527         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_458292, whole genome shotgun sequence.
ACCESSION   DASVLN010000527 DASVLN010000000
VERSION     DASVLN010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458292"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2489
                     /locus_tag="VF453_12815"
     CDS             <1..2489
                     /locus_tag="VF453_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7688585.1"
                     /translation="AEVAELAAIDDQSGSVLDRPFDDLVGELARARAERRLEGLRLGP
                     WRLVRRIASGGMGEVFLGERADGAFEMQVAVKLLRDGLVDDADVERFARERQTLARLE
                     HVNLVRILDGGVSEAGLPYFVMELVDGEPIDAWCERHGATLAQRLALLHEVCGVVHYV
                     NQRGIVHRDLKPRNILVTPCGVVKLVDFGIATRLAEPGAAPDPTRTALRGMTIQYASP
                     EQIRGDTVDAASDVYALGVVMVKLLAGAQPYAVDPSTASGFELAKAICDAEPRRMSEL
                     VPEGPGAAARRRALAGDLDAIAAKALRKDRATRYQTAAELDDDLFRHRSRLPVRARRG
                     AFSYRFGRWLVKHRALAGAAIVSNIVLLAGISVTLAEVHEARAQRERANRNYASVRAL
                     TKDFLFELDEAIAALPGSLPARKLVVGKAQQYLQTLAGQARQGTGADADGALDLDLGI
                     SYRKLGDLQDFPGRPNLGNPDAALADYAQSVRLLEPLAAEGLEPHASADVILGAHRAL
                     VLTWLSRASVLSANARPDEAEAALRSGEAVLGELMQLVPPDTETDRLRASVYGGLSWV
                     LHEKGDVAAFERYADQSEAILERIVAAHPDDTVSLMRLQSSYARRTADYAGRPPTPRN
                     LQRWLAAARKWQGIGQRLLARDANDPRYLPVAAEAEMFLGRAYARLGDRRNAAAAIDE
                     GIGQLARELRLDPDDDAALQIMTELHLARAHLQLDARDPGGARAEARQALDFLARRSQ
                     AARADGYDLVTAAQLHHVVAEAWHQDAERDRARPAAVSRDHANACDEWRAADALLRQV
                     PDPSVISTDGYDPGDIRRGAAACRTARLPA"
     gene            2665..>2864
                     /locus_tag="VF453_12820"
     CDS             2665..>2864
                     /locus_tag="VF453_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688586.1"
                     /translation="MMYEHPVRREEGRTPPVFIHDDPPPVLYPPARGTAIPSPLAGLV
                     AARDAAERRRAIDDMLLAIGCD"
BASE COUNT          400 a         1094 c         1025 g          345 t
ORIGIN      
        1 cggccgaggt ggccgagctc gcggccatcg acgaccagag cggatccgtc ctcgaccgcc
       61 ctttcgacga cctcgtcggc gagctcgccc gcgcgcgcgc cgagcgccgc ctggagggcc
      121 tgcggctcgg gccgtggcgg ctggtgcggc gcatcgccag cggcggcatg ggcgaggtct
      181 tcctcggcga gcgcgcggac ggcgccttcg agatgcaggt cgccgtcaag ctgctgcgcg
      241 acgggctggt cgacgacgcc gacgtcgagc gcttcgcccg cgagcgccag accctcgccc
      301 ggctcgagca cgtcaacctc gtgcgcatcc tggacggcgg cgtcagcgag gcggggctgc
      361 cctacttcgt gatggagctg gtcgacggcg agcccatcga cgcctggtgc gagcgccacg
      421 gcgcgacgct cgcccagcgc ctcgcgctgc tgcacgaggt ctgcggcgtc gtgcactacg
      481 tcaaccagcg cggcatcgtc catcgcgacc tgaagccgcg caacatcctc gtcacgccct
      541 gcggcgtggt caagctcgtg gacttcggca tcgccacgcg cctggccgag cccggtgcgg
      601 cgcccgatcc cacgcgcacc gcgctgcgcg ggatgacgat ccagtacgcg agccccgagc
      661 agatccgcgg cgacaccgtc gacgccgcga gcgacgtgta cgcgctgggc gtcgtgatgg
      721 tcaagctgct cgcgggcgcg cagccctacg cggtcgaccc gtcgacggcc tcgggcttcg
      781 agctcgccaa ggcgatctgc gacgcggagc cgcgccgcat gagcgagctg gtgcccgagg
      841 gtccgggcgc ggcggcgcgg cggcgcgcgc tggccggcga cctcgacgcg atcgccgcca
      901 aggcgctgcg caaggaccgc gcgacgcgct accagaccgc cgcggagctc gacgacgacc
      961 tgttccgcca ccgctcgcgc ctgcccgtgc gcgcgcgccg cggggcgttc agctaccgct
     1021 tcggccgctg gctggtcaag catcgcgcgc tcgcgggcgc ggcgatcgtg tccaacatcg
     1081 tgctgctggc gggcatcagc gtcacgctcg ccgaggtgca cgaagcgcgc gcccagcgcg
     1141 agcgcgcgaa ccgcaactac gcgagcgtgc gggcgctgac caaggacttc ctgttcgagc
     1201 tcgacgaggc gatcgccgcc ctgcccggct cgctgcccgc gcgcaagctg gtggtcggca
     1261 aggcgcagca gtacctgcag acgctggcgg gccaggcgcg ccagggcacg ggcgccgacg
     1321 ccgatggcgc gctggacctc gacctgggca tcagctaccg caagctcggc gacctgcagg
     1381 acttccccgg gcgccccaac ctcggcaacc cggacgcggc actcgccgac tacgcgcagt
     1441 cggtgcgcct cctggagccg ctcgccgccg agggcctcga gccgcatgcg agcgcggacg
     1501 tcatcctggg ggcgcatcgc gcgctcgtgc tcacgtggct cagccgcgcg tcggtgctga
     1561 gcgccaatgc gcggcccgac gaggcggagg ccgcgctgcg atcgggcgag gcggtgctcg
     1621 gcgagctgat gcagctggtg ccgcccgaca ccgagaccga ccgcctgcgc gcctccgtgt
     1681 acggcggcct gagctgggtg ctgcacgaga agggcgacgt cgcggcgttc gagcgctacg
     1741 ccgaccagag cgaggccatc ctcgaacgga tcgtcgcggc gcatccggac gacaccgtca
     1801 gcctgatgcg gctgcagtcc tcctacgcgc ggcgcaccgc cgactacgcc ggccggccgc
     1861 cgacgccccg gaacctgcag cgctggctgg cggccgcgcg caagtggcag ggcatcggcc
     1921 agcggctgct cgcgcgcgac gcgaacgatc cgcgctacct gcccgtcgcg gccgaggcgg
     1981 agatgttcct cgggcgcgcc tacgcgcgcc tgggcgaccg ccgcaacgcc gccgcggcga
     2041 tcgacgaagg catcggccag ctggcgcgcg agctgcgcct ggatccggac gacgacgcgg
     2101 ccctgcagat catgacggag ctgcacctcg cgcgcgccca cctgcagctc gacgcgcgcg
     2161 atccgggggg cgcgcgcgcc gaggcgcggc aggctctcga cttcctcgcc cgccgctcgc
     2221 aggccgcgcg cgccgacggc tacgacctgg tcaccgcggc ccagctccac cacgtcgtgg
     2281 ccgaggcctg gcaccaggac gccgagcgcg accgcgcccg cccggcagcg gtgtccaggg
     2341 accacgcgaa cgcctgcgac gaatggcgcg ccgccgacgc gctgctgcgc caggtgcccg
     2401 atccctcggt gatctcgacc gacggctacg accccggcga catccggcgc ggcgccgcgg
     2461 cctgccgcac ggcgcgcctg cccgcctgat cacgcaatcg accaagacga gcatttcttc
     2521 acgaatgcgg gcggcgcgat cggccgttca tcttgcgtca agggaaatta atgcgtatcg
     2581 cgacgtaacg aaatgagcct tcggccgtcc gtttctcgct aatgaagagg agaaccagaa
     2641 aacggactga gacaggccca caccatgatg tacgagcacc ccgtgcgacg cgaggaaggc
     2701 cgcacccccc cggtcttcat ccacgacgac ccgccgcccg tcctgtatcc gccggcgcgc
     2761 ggcacggcga tcccgagccc gctcgcaggg ctggtcgccg cgcgcgacgc ggcggagcgg
     2821 cggcgcgcca tcgacgacat gctgctggcc atcggctgcg actg
//
LOCUS       DASVLN010000528         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_188623, whole genome shotgun sequence.
ACCESSION   DASVLN010000528 DASVLN010000000
VERSION     DASVLN010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188623"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..961
                     /locus_tag="VF453_12825"
     CDS             206..961
                     /locus_tag="VF453_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688587.1"
                     /translation="MTVARIAASCALAATLLAGVAPARALGVPMDDQALSDTWGQALL
                     DLTNTTDGQYQFSRLTFNADVTLSMNLNGLKLGTQANGTSDIDIATMNFGRSDATDAQ
                     RTVAISKPYFEWVYSGTAGSADRQVVGMRLGFGSMTGDLGLSMNTVSGALSFASPDGT
                     TTYSANGKGTTTLTSSSGGSLALNQVGGVTADASRDFFISVLKSAVAFPGTDASMAAP
                     AQSQAGFWLNWTDRLKAINTTGQTLPNVPKIGP"
     gene            1087..1923
                     /locus_tag="VF453_12830"
     CDS             1087..1923
                     /locus_tag="VF453_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688588.1"
                     /translation="MKPLDDAALAEVRGADGISFNLNNFSLTSIPSLPLQLTYQSTNG
                     SSMTLAGLDLSRTDDPDPFADPYTLSVLKRPGFQDGIALDFPLNAAGNQAWTLTADFS
                     NCDLGTSAACSGTNFVGGTLQAKGLVMRGGGLYLAPSAIADIQGIAFGVGTQLDIGSL
                     AIYSHGRTADDQPDAADSMTLTGIHLANASGGPWMLADLTYHPGLFNAQTDATGSYLH
                     LQIGWPTSSSYQVPAASIRIDDITFTTPGAGGTPVQTSLGSASIASLQINYADIRFRT
                     GN"
     gene            1938..2429
                     /locus_tag="VF453_12835"
     CDS             1938..2429
                     /locus_tag="VF453_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688589.1"
                     /translation="MRALCQPRLAARLAAGIVLAAAGVAHAAKVPMSDAELSGVSGQG
                     VAILVHLELNAGLLTGAPIDSRFYAGFDVGGTTTYAIAQNFGGLLNLFAITLDPSAAP
                     DGTGYLAIGMPNYLSAKDFGVRAIGVQVDPKGPINNSLGSLLLNGSANMTGQLNVWPT
                     ANK"
     gene            2476..3243
                     /locus_tag="VF453_12840"
     CDS             2476..3243
                     /locus_tag="VF453_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX7688590.1"
                     /translation="MLLIFLGVIAAAIAGAGVLSKNEPLNHPQLEYELNVSKANGGLE
                     PESVQMYPYQYYKYKDVVHQAFDYSCGSAALTTILEFHLGLPVTEKDAMEGMLEHGEK
                     DKIIARRGFSLLDMKRYVATLGSDSGGFKADLSDLEKLDEPAIVPINYAGFKHFVVVK
                     GVRGGKVFIADPSAGNIAFSEHEFAGLWDNGTLFVIYPSKTHPHVQSSLALTDQELGV
                     ISPDLVRPDVALRPVDNRDALQRAVESQLGTLQYKRR"
BASE COUNT          547 a         1304 c         1032 g          483 t
ORIGIN      
        1 accaaggtct ggattttcgg ccactgatcg gcgacgacga gacacgcact tctccacgga
       61 gtgcgtgaaa gtctgttcta caggcctcgc gacgtcgcgt cacgaggcct tttccttctt
      121 tgattcgggc gcgagaacca gtacgctccc gcctccggcc cggcgccccg ccgcagccgc
      181 cgcctccacc cagcccggac gccccatgac cgtcgcccgc atcgccgcct cctgcgccct
      241 ggccgccacc ctgctggccg gcgtcgcgcc cgcgcgcgcg ctgggcgtgc cgatggacga
      301 ccaggcgctt tccgacacct ggggccaggc cctcctcgac ctgaccaaca cgaccgacgg
      361 ccagtaccag ttctcgcggc tgacgttcaa cgccgacgtc acgctgtcga tgaacctgaa
      421 cgggctcaag ctcggcacgc aggccaacgg cacgtccgac atcgacatcg cgacgatgaa
      481 cttcggccgc agcgatgcca cggacgcgca gcgcacggtc gcgatctcca agccgtactt
      541 cgaatgggtc tactccggca ccgccggctc agccgaccgc caggtcgtcg gcatgcgcct
      601 gggcttcggc agcatgaccg gtgacctcgg gctgtcgatg aacacggtca gcggcgcgct
      661 gagcttcgcc agccccgacg gcacgacgac ctattccgcc aacggcaagg gcacgacgac
      721 gctcacgagc tcgtcgggcg gcagcctcgc gctgaaccag gtcggcggcg tcacggcgga
      781 cgcgagccgc gacttcttca tctcggtgct gaagtcggcg gtcgccttcc cgggcaccga
      841 cgcgtcgatg gccgcgcccg cgcagtcgca ggccggcttc tggctgaact ggaccgatcg
      901 cctcaaggcc atcaacacca cgggccagac gctgcccaac gtgcccaaga tcgggccgtg
      961 atgcggcgcg cgacgatcca cgcccgcccc accccggccc cggccgcagg cgggctccgg
     1021 atcgccctga cctcgacctg cgtcgccctc gccgtctgcg gcgcggcgcc cgcgcgcgcc
     1081 ggcggcatga agccgctcga cgatgccgcg ctggccgagg tgcgcggcgc cgacggcatc
     1141 tcgttcaacc tgaacaattt ctcgctcacc agcatcccgt ccctgccgct gcagctgacc
     1201 taccagtcga ccaacggcag ctcgatgacg ctggcgggcc tggatctgtc gcgcaccgac
     1261 gatcccgacc ccttcgccga tccgtacacg ctgtcggtgc tcaagcgccc cggcttccag
     1321 gacggcatcg cgctggactt cccgctcaac gcggccggca accaggcgtg gacgctcacg
     1381 gcggacttct ccaactgcga cctcggcacc tcggcggcgt gcagcggcac gaacttcgtc
     1441 ggcggcacgt tgcaggccaa gggcctcgtg atgcgcggcg gcggcctcta tctcgcaccg
     1501 tccgcgatcg ccgacattca gggcatcgcg ttcggcgtcg gcacgcagct ggacatcggc
     1561 agcctcgcga tctactcgca cggccgcacc gcggacgacc agcccgacgc cgccgactcg
     1621 atgacgctca ccggcatcca cctcgccaac gcgtccggcg gcccgtggat gctggcggac
     1681 ctcacctacc atccgggcct gttcaacgcg cagaccgatg ccaccggctc ctacctgcac
     1741 ctgcagatcg gctggcccac cagcagctcc taccaggtgc cggcggcgag cattcgcatc
     1801 gacgacatca cgttcacgac gcccggcgcg ggcggcacgc cggtgcagac cagcctcgga
     1861 tcggccagca tcgcgagcct gcagatcaac tacgcggaca tcaggttccg caccggcaac
     1921 tgaccgccgc ccgccgcatg cgcgcccttt gccaaccccg cctcgccgcg cgccttgccg
     1981 ccggcatcgt ccttgcggcg gccggggtcg cccatgcggc gaaggtgccg atgtcggacg
     2041 ccgagctgag cggcgtctcc ggccagggcg tcgcgatcct cgtgcacctg gagctcaacg
     2101 ccggcctgct gaccggcgcg cccatcgaca gccgcttcta cgccggcttc gacgtcggcg
     2161 gcacgaccac ctacgcgatc gcgcagaact tcggcggcct gctgaacctg ttcgcgatca
     2221 cgctcgaccc gagcgccgcc cccgacggca ccggctacct cgccatcggc atgcccaact
     2281 acctgtccgc gaaggacttc ggcgtgcgcg cgatcggcgt gcaggtcgac ccgaaggggc
     2341 cgatcaacaa cagcctgggc tcgctgctgc tcaacggatc ggcgaacatg acgggccagc
     2401 tcaacgtgtg gcccacggcg aacaagtgac gccgaccgcc gcgcccgtgc aaaacatcca
     2461 gggaacagac ttcgcatgct gctcatcttc ctcggcgtca tcgccgccgc gatcgccggc
     2521 gccggcgtcc tgtccaagaa cgaaccgctg aaccacccgc agctggaata cgagctcaac
     2581 gtctccaagg ccaacggcgg gctcgagccc gagtcggtgc agatgtaccc gtaccagtac
     2641 tacaagtaca aggatgtcgt ccaccaggcg ttcgactaca gctgcggctc ggcggcgctc
     2701 acgaccatcc tggagttcca cctcggcctg ccggtgaccg agaaggacgc gatggagggc
     2761 atgctggaac acggcgagaa ggacaagatc atcgcccgcc gcggcttctc gctcctggac
     2821 atgaagcgct acgtcgcgac gctgggcagc gactcgggcg gcttcaaggc cgacttgtcc
     2881 gacctcgaga agctcgacga gccggcgatc gtgccgatca actacgccgg cttcaagcac
     2941 ttcgtggtgg tcaagggcgt gcgcggcggc aaggtgttca tcgccgatcc gtccgccggc
     3001 aacatcgcgt tctccgagca cgagttcgcg ggcctgtggg acaacggcac gctgttcgtc
     3061 atctatccgt cgaagacgca tccgcacgtg cagtccagcc tcgcgctgac cgaccaggag
     3121 ctcggcgtga tctcgcccga cctcgttcgc ccggacgtgg ccctgcgtcc cgtggacaat
     3181 cgcgacgcgc tgcaacgcgc agtggagtcg caactcggca cccttcaata caaacgtcga
     3241 tgaacatgaa cccggcatcc cggctcgccg cggccctggc cgccgcctgc ggcctcctcg
     3301 cctgcgcgcc cgcatccgcg cagcctgaca accaacccac ccccgccggc cccgccgcgc
     3361 ccgacg
//
LOCUS       DASVLN010000529        10465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_458304, whole genome shotgun sequence.
ACCESSION   DASVLN010000529 DASVLN010000000
VERSION     DASVLN010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458304"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..3504)
                     /locus_tag="VF453_12845"
     CDS             complement(244..3504)
                     /locus_tag="VF453_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7688591.1"
                     /translation="MNLSKFFIDRPIFAGVLSLLIFLGGLIAVRGLPVSEYPNVVPPS
                     VVVRANYPGANPQVIAETVATPIEEAINGVEGMLYMSSQATTDGLMTLTVTFKLGTDP
                     DKAQQLVQNRVAQAEPRLPEEVRSLGLTTIKSSTDLTMVVHLLSPTGRYDMTYLRNYA
                     VLNVKDRLARVEGVGDVQLFGSGDYSMRVWLDPQKVAEHGLSAGDVVAAIREQNVQAA
                     AGIVGASPGLNGIDVQLSVNAQGRLQTEQEFGDIIVKSGADGAVTRLKDVARIELGAA
                     DYSLRSLLDNKDAVASPIFAAPGSNAIAISNGVRATMAEIKKNMPDGVDYEIVYDPTQ
                     FVRASIESVVHTLLEAIALVVLVVILFLQTWRASIIPLLAVPVAVVGTFAVMSLSGFS
                     INALSLFGLVLAIGIVVDDAIVVVENVERNIESGLSPRDATYRAMREVSGPIIAIALV
                     LVAVFVPLGFITGLTGQFYKQFALTIAMSTVISAVNSLTLSPALAAMLLKGHDAPKDA
                     LTRGMDWALGGFFRRFNALFARGSAAYGGGVGKAISRKAVMFGIYVLLAVSTLGLFHA
                     VPKGFVPAQDKQYLIGFAQLPDGATLDRTEDVIRRMGEITLKQPGVEHAVSFPGLSIN
                     GFTNSSNSGIVFVTLKPFDERKGAALSAGAIAQALNQKYGAIQDAFIVMFPPPPVQGI
                     GTTGGFKLQLEDRGSVGIAGMDAALKAFMAKAYQAPELAGLFSSYQVNVPQLYADIDR
                     TKARQLGVPITDIFDTMQIYLGSLYANDFNKFGRTYTVRVQADAAYRARAEDIGLLKV
                     RSTTGEMVPLSALMKVTPTFGPERAMRYNGFLTADVNGGPAPGYSSGQAQEAIKRIAA
                     ETLPPGVSYEWTDLTYQEILAGNSAVLIFPLAILLVFLVLAAQYESLTLPLAILLIVP
                     MGLLAAMTGVWLTRGDNNVFTQIGLMVLVGLSAKNAILIVEFARELEFAGRTPMRAAI
                     EAAGLRLRPILMTSMAFIMGVLPLVLSTGAGSEMRHAMGVAVFFGMIGVTAFGLFLTP
                     VFYVLLRALAGNRPLKQHGEVPHDVEAPGVMVLHGGHGHAGGGPLPVPSAPRHPEE"
     gene            complement(3666..4850)
                     /locus_tag="VF453_12850"
     CDS             complement(3666..4850)
                     /locus_tag="VF453_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7688592.1"
                     /translation="MTQVPLQARRRLWLAGTTLTALAAVFATVFLLRAAPAGAAGASA
                     APPAVPVSVATVSRSDVATWDEFSGRLEAVERVDIRPRVAGTVEKVHFREGMLVKAGD
                     LLVTIDPAPYQADVDRAEAGVVAAQARFTQARTDYDRAKRLLDDHAISQREYDQASNA
                     QNEADANLRAARAALTSAKLSLGYTEVRAPVAGRVGRLEITTGNLVAAGPGAPVLTTL
                     VSVSPIYASFDADEGVVAKALRELPPGTGRTHLDEIPVQMGTSSDADTPIKGHLQLVD
                     NQVDARSGTVRVRAVFDNADGTLIPGQFARLRMGSPKAEPAILISERAVGTDQNKKFV
                     MVVDGDNKVAYREVTLGANVDGLRIVTSGLKAGERVVVNGLQRVRPGALVAPQEVKMK
                     GA"
     gene            5047..5745
                     /locus_tag="VF453_12855"
     CDS             5047..5745
                     /locus_tag="VF453_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7688593.1"
                     /translation="MKKRPEERAAPADEAADDAATIAVAEPTAAVAKPARRPRGRPLS
                     FDRDAALEAAMHVFWERGYEAASVADLTSAMGITPPSLYTAFGDKEQLFLEAIERYAL
                     GYGSISARALREEPTARGAIERWLVEAAKELTEPCHPKGCMVVMAATNCSAAAERVQD
                     ALMLRRTEAIANVGRRIQEAIDSGELPADTDAAELASFYAAVYQGMSMQAKDGATREG
                     LLRTVRTAMRAWPA"
     gene            5925..6560
                     /locus_tag="VF453_12860"
     CDS             5925..6560
                     /locus_tag="VF453_12860"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7688594.1"
                     /translation="MTFTKSLVAIAIASVGGAAFAAPTTYTGYDNSVSAIGANAAAAA
                     ASFNSAAPGTSVIDFESAVPAGVSISGGSITNNSGCGALCGFNTTSGGQNFLLLFGGS
                     ATFTFATPVNAFGFYVSGLQTDLVPTETLTFSDGSSQTINIGPSTGGGDGFVGFTDLS
                     GVSSVTYNATNDIVAIDDVRYGALSAVPEPASFALFGAGLLGLGLARRRRG"
     gene            complement(6640..7626)
                     /locus_tag="VF453_12865"
     CDS             complement(6640..7626)
                     /locus_tag="VF453_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7688595.1"
                     /translation="MKTRSLGRSGLQVSALGLGCMGMTHAYFPMPDRAEMVALLRQAV
                     DLGVTFFDTAEIYGPYTNEELLGEALAPVRERVVIATKFGFDVDRMPGVDSRPERIRA
                     VCEASLKRLRTDAIDLFYQHRPDPKVPVEDVAGTVKDLIREGKVRHFGLSESDAATIR
                     RAHAVQPVAALQSEYSLWLRDLEGTILPVLEELGIGLVPFSPLGRGYLTGKMDETTPL
                     QASDFRASLPRFTPEARKANAALVELLKATASRLGATPAQVALAWVLARKPWIVPIPG
                     TTKLNRLEENLAAADLELTPSDVQEIDSAAEAIRIEGARYSEAQQRFVDAKP"
     gene            complement(7920..8594)
                     /gene="rpe"
                     /locus_tag="VF453_12870"
     CDS             complement(7920..8594)
                     /gene="rpe"
                     /locus_tag="VF453_12870"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX7688596.1"
                     /translation="MNPLVVAPSILSADFGRLADEVRAVDAAGADWIHVDVMDGRFVP
                     NITIGPVVVEAIRRATTKPLNVHLMIVEPERMIDDFIAAGADHLLVQSEPASTIHLHR
                     VLSRIREQGRKAGVVLDPASPVAFVEHVLHLVDVVLVMTVNPGFGGQRFLPETLPKIT
                     ALRRMCDARGLNPYIEVDGGQNGGNAWQAVEAGADALVAGSAVFGSGDYARAIAALRE
                     HRAPAA"
     gene            complement(8597..9682)
                     /gene="tal"
                     /locus_tag="VF453_12875"
     CDS             complement(8597..9682)
                     /gene="tal"
                     /locus_tag="VF453_12875"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX7688597.1"
                     /translation="MNRITRQLHDLGQSLWIDNISREGLHSGHLRRLIDEFSITGLTS
                     NPTIFEKAMGQGHAYDDAIARCARDGRSTEELFFQLALEDLRAAADLFRPTWEATGGI
                     DGWVSLELSPTLARDTRGSIQAAASLHAQAARENLFIKIPGTPEGLAAIEESTYKGVP
                     VNVTLLFSRDQYLAAADAYMRGLERRLDDGKSAVVESVASLFVSRWDVAVQDQVAAQY
                     RNRLGVAIGMDCYRAYREVLASGRWQRLAEAGAHPQRLLFASTGTKDKDASPTLYVEG
                     LAAPDTIDTMPDKTLEAFAERGVVGAPLPEDGGHAAAVLDEFRREGVDLEALALRLQR
                     EGADAFVKSWNALVAGLERKVRELAAA"
     gene            complement(9654..10382)
                     /locus_tag="VF453_12880"
     CDS             complement(9654..10382)
                     /locus_tag="VF453_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7688598.1"
                     /translation="MTVPADDVVFLLDVDNTLLDNDRIVADLREHLAATFGAACADRY
                     WALFEQLRDELGYVDYLGALQRYRHEVEGHGADAQALLRMSGFLVDYPFRDRVYPGVF
                     ELLETLARHGPTVILSDGDVVFQPRKIERSGLWDAVRGRVLVYVHKERMLDEVRRTYP
                     ARRYVLVDDKRRILAQVKDAWGDGVTTVCPRQGHYADDPSELARRPLPDVVVERIGEL
                     ARMNIAALAGAPGARHEPNHPTAA"
BASE COUNT         1548 a         3645 c         3751 g         1521 t
ORIGIN      
        1 ctgggcgccg gccggggcga cggcgacgaa gcgggcgccg ccgtcctcct tgaacgccgc
       61 cggcgcggcg ggcgcggccc ggggcgtgaa gtcgggggtg ctcgcgcagc cggccagcac
      121 gagggcggcg accagcggca cgagcggggt acggaagaag cggttcatga caattccttg
      181 ggacgggagg acggcgcgcc gcacatacac cttggggtat ggtcgcgcgc cgcccggttc
      241 agctcattcc tcgggatggc gcggcgccga gggcaccggc agcgggccgc cgccggcatg
      301 gccgtggccg ccgtgcagca ccatcacgcc cggggcttcg acatcgtgcg gcacctcgcc
      361 gtgctgcttg agcgggcggt tgcccgccag ggcgcgcagc agcacgtaga acaccggcgt
      421 caggaacagg ccgaacgcgg tcacgccgat catcccgaag aacacggcca cgcccatcgc
      481 gtgccgcatc tcggagccgg cgccggtgga cagcaccagc ggcagcacgc ccatgatgaa
      541 ggccatcgag gtcatcagga tcgggcgcag ccgcagcccc gccgcctcga tcgccgcgcg
      601 catcggcgtg cggcccgcga actcgagctc gcgcgcgaac tccacgatca ggatcgcgtt
      661 cttcgcgctc aagccgacca gcaccatcag gccgatctgc gtgaagacgt tgttgtcgcc
      721 gcgcgtgagc cacacgcccg tcatcgcggc cagcaggccc atcggcacga tcagcaggat
      781 cgccagcggc agcgtcaggc tctcgtactg cgcggccagc accaggaaca cgagcaggat
      841 cgcgagcggg aagatcagca ccgcggagtt gccggccagg atctcctggt acgtcaggtc
      901 cgtccactcg tagctcacgc cgggcggcag cgtctcggcg gcaatgcgct tgatcgcctc
      961 ctgcgcctgg ccggaggagt agcccggcgc cgggccgccg ttgacgtcgg cggtcaggaa
     1021 gccgttgtag cgcatcgcgc gttccggccc gaaggtcggc gtgaccttca tcagcgcgga
     1081 cagcggcacc atctcgcccg tcgtggagcg caccttcagc aggccgatgt cctcggcgcg
     1141 cgcgcggtag gcggcgtccg cctgcacgcg cacggtgtac gtgcggccga acttgttgaa
     1201 gtcgttcgcg tacaggctgc cgaggtagat ctgcatcgtg tcgaagatgt ccgtgatcgg
     1261 caccccgagc tggcgcgcct tcgtgcggtc gatgtcggcg tacagctgcg gcacgttgac
     1321 ctggtagctc gagaacaggc ccgccagctc cggcgcctgg taggccttgg ccatgaaggc
     1381 cttgagcgcc gcgtccatgc cggcgatgcc caccgagccg cgatcctcca gctgcagctt
     1441 gaagccgccc gtggtgccga tgccctgcac cggcggcggc gggaacatca cgatgaaggc
     1501 gtcctggatc gcgccgtact tctggttcag cgcctgcgcg atcgcgccgg ccgacagcgc
     1561 cgcgcccttg cgctcgtcga agggcttgag cgtgacgaac acgatgcccg agttggagct
     1621 gttggtgaag ccgttgatcg acaggcccgg gaacgacacc gcgtgctcga cgcccggctg
     1681 cttgagcgtg atctcgccca tgcggcggat gacgtcctcg gtgcggtcca gcgtcgcgcc
     1741 gtcgggcagc tgcgcgaagc cgatcaggta ctgcttgtcc tgcgccggca cgaagccctt
     1801 gggcacggcg tggaacaggc ccagcgtcga gaccgccagc agcacgtaga tgccgaacat
     1861 caccgccttg cgcgagatcg ccttgcccac gcccccgccg taggccgcgg agccgcgcgc
     1921 gaacagcgcg ttgaagcggc ggaagaagcc gcccagcgcc cagtccatgc cgcgggtgag
     1981 cgcatccttg ggcgcgtcgt ggcccttgag cagcatggcc gcgagcgccg gcgacagcgt
     2041 cagcgagttg accgccgaga tcaccgtcga catcgcgatc gtcagcgcga actgcttgta
     2101 gaactgcccc gtgaggccgg tgatgaagcc cagcggcacg aacacggcca ccagcaccag
     2161 cgcgatcgcg atgatcgggc cggagacctc gcgcatcgcg cggtaggtcg cgtcgcgcgg
     2221 cgacaggccg ctctcgatgt tgcgctcgac gttctccacg accacgatcg cgtcgtcgac
     2281 gacgatgccg atcgccagca cgaggccgaa cagcgacagc gcgttgatcg agaagcccga
     2341 caggctcatc accgcgaacg tgccgaccac ggcgaccggc accgccagca gcgggatgat
     2401 ggacgcgcgc caggtctgca ggaacaggat cacgacgagc acgaccagcg cgatcgcctc
     2461 gagcagcgtg tgcacgacgg actcgatgga cgcgcgcacg aattgcgtcg ggtcgtagac
     2521 gatctcgtag tccacgccgt cgggcatgtt cttcttgatc tcggccatcg tggcgcgcac
     2581 gccgttggag atcgcgatcg cgttggagcc cggcgccgcg aagatcgggg acgcgacggc
     2641 gtccttgttg tccaggagcg agcgcagcga gtagtcggcc gcacccagct cgatgcgcgc
     2701 gacgtccttc aggcgggtga ccgcgccgtc cgcgccggac ttgacgatga tgtcgccgaa
     2761 ctcctgctcg gtctgcaggc ggccctgcgc attgaccgac agctgcacgt cgatgccgtt
     2821 caggcccggc gacgcgccga cgatgccggc cgcggcctgc acgttctgct cgcggatcgc
     2881 ggcgacgacg tcgcccgccg acaggccatg ctcggcgacc ttctgcgggt cgagccagac
     2941 gcgcatcgag tagtcgccgg agccgaacag ctgcacgtcg cccacgccct cgacgcgcgc
     3001 gaggcggtcc ttgacgttga gcaccgcgta gttgcgcagg taggtcatgt cgtagcggcc
     3061 ggtcggcgac agcaggtgca cgaccatcgt gaggtcggtc gagctcttga tcgtcgtcag
     3121 gccgagcgag cgcacctcct cgggcaggcg cggctccgcc tgcgcgacgc ggttctgcac
     3181 cagctgttgc gccttgtccg gatcggtgcc cagcttgaag gtcaccgtca gcgtcatgag
     3241 gccgtcggtc gtcgcctggc tgctcatgta gagcatgccc tcgacgccgt tgatggcctc
     3301 ctcgatcggc gtggccacgg tctcggcgat cacctgcggg ttggcgccgg ggtaattcgc
     3361 gcgcacgacc acggagggcg gcacgacgtt ggggtattcg gagacgggca gcccgcgcac
     3421 ggcgatgagg ccgccgagga agatcagcag cgatagcacg ccggcgaaga tgggccggtc
     3481 gatgaagaac ttggagaggt tcatggaacg ctcctgggaa cgtcgggaga atttcggaac
     3541 gaacgtcgcc gccgccggcg ctcgaacgtc ggcgaggaaa gtcgggggag ggaggcgacg
     3601 ggcttggccc gcgccgaacc ggccggcccc ctcggggggc agcgaccgtc agggagcgtg
     3661 ggggctcacg ctcccttcat cttcacttcc tgcggcgcga ccagcgcgcc cggccgcacg
     3721 cgctgcaggc cgttgacgac cacgcgctcg cccgccttca ggccggaggt gacgatgcgc
     3781 aggccgtcga cgttggcgcc cagcgtgacc tcgcggtacg cgaccttgtt gtcgccgtcg
     3841 acgaccatca cgaacttctt gttctggtcg gtgccgaccg cgcgctcgct gatcaggatc
     3901 gccggctcgg ccttggggct gcccatgcgc aggcgggcga attggccagg gatgagcgtg
     3961 ccgtcggcgt tgtcgaacac cgcgcgcacg cgcaccgtgc cgctgcgcgc gtcgacctgg
     4021 ttgtcgacga gctgcaggtg acccttgatc ggcgtgtcgg cgtcggacga ggtgcccatc
     4081 tgcaccggga tctcgtcgag gtgcgtccgg cccgtgccgg gcgggagctc gcgcagagcc
     4141 ttggcgacca cgccctcgtc ggcgtcgaag ctcgcgtaga tcgggctcac cgacaccagc
     4201 gtcgtcagca cgggcgcgcc gggccccgcc gccaccaggt tgccggtggt gatctcgagc
     4261 ctgccgacgc ggcccgcgac cggcgcgcgc acctcggtgt agccgagcga cagcttggcc
     4321 gaggtcagcg ccgcgcgcgc cgcgcgcagg ttggcgtcgg cctcgttctg ggcgttgctg
     4381 gcctggtcgt actcgcgctg cgagatcgca tggtcgtcga gcagccgctt ggcgcggtcg
     4441 tagtcggtcc gggcctgggt gaagcgagcc tgcgcggcca cgaccccggc ttcggcgcgg
     4501 tcgacgtcgg cctggtacgg cgcggggtcg atcgtgacca gcaggtcgcc ggccttgacc
     4561 agcatgccct cgcggaagtg caccttctcg accgtgccgg ccacgcgcgg acggatgtcg
     4621 acgcgctcga ccgcctcgag gcggccggag aactcgtccc aggtggcgac gtcgctgcgc
     4681 gagacggtcg cgacggacac cggcacggcc gggggcgccg ctgatgcccc cgcagcgccc
     4741 gcgggtgcgg cgcgcaacag gaagacggtg gcgaagacgg ccgcgagcgc cgtcagcgtg
     4801 gtgccggcca gccagagtcg gcggcgggct tggagcggaa cctgggtcat tgcggaactc
     4861 ctggcgagtc gagagggggc gaaagacggg aagccgggga accttcgggc gatgcggcgg
     4921 ctcgaccagg gaggccctgg ccttgcgctg cgtgtccgtg aaagttttat cgtagcgatc
     4981 actacgacat gtggcacaat gtacccagcg cgcaggacgc cgtcaactaa tttcgtagtg
     5041 tcaattatga aaaagaggcc cgaagaacgc gccgccccgg ccgacgaggc cgcggacgac
     5101 gccgcgacga tcgccgtcgc ggaacccacc gccgcggtgg ccaagccggc gcgcaggccg
     5161 cgcgggcgcc cactgtcgtt cgaccgcgac gccgcgctcg aggccgcgat gcacgtgttc
     5221 tgggagcgcg gctacgaggc ggcctcggtc gccgacctga cctcggccat ggggatcacc
     5281 ccgcccagcc tgtacaccgc gttcggcgac aaggagcagc tgttcctgga ggcgatcgag
     5341 cgctacgccc tcggctacgg cagcatcagc gcgcgcgcgc tgcgggagga acccaccgcg
     5401 cgcggcgcga tcgagcgctg gctggtcgag gccgccaagg agctcaccga gccctgccat
     5461 cccaagggct gcatggtggt gatggcggcg accaactgct ccgcggccgc cgaacgcgtg
     5521 caggacgcgc tgatgctgcg ccgcacggag gcgatcgcga acgtcggccg ccgcatccag
     5581 gaggccatcg actccggcga gctgcccgcc gacacggacg ccgcggagct cgcgagcttc
     5641 tacgccgccg tctaccaggg catgtcgatg caggccaagg acggcgcgac gcgcgagggc
     5701 ctgctgcgca cggtgcgcac cgcgatgcgt gcgtggccgg cctgagtccg gcggcccccc
     5761 aagtttgaac cgccgcgagc tcccccaaac cggggagcta acgagtcctt gcgtcgcttt
     5821 gccgccgcct tctccctaac tcaggggaat cgggcgcggg cgctcttgag cagaattggc
     5881 accggcttcc cgaaccgatc acttctcact caaggacacg ctccatgacc ttcaccaagt
     5941 ccctggtcgc catcgcgatc gcttccgtcg gcggcgcagc cttcgccgct ccgaccacct
     6001 acaccggata cgacaacagc gtctccgcga tcggcgcgaa cgccgccgcc gcggccgcca
     6061 gcttcaacag cgcggcgccc ggcacctccg tgatcgactt cgagtcggcc gtgccggcgg
     6121 gcgtctcgat ctccggtggc agcatcacca acaactccgg ctgcggcgcg ctgtgcggct
     6181 tcaacaccac gtcgggcggc cagaacttcc tgctgctgtt cggcggcagc gccaccttca
     6241 cgttcgcgac gccggtcaac gcgttcggct tctacgtgag cggcctgcag accgacctgg
     6301 tcccgaccga gacgctgacc ttcagcgacg gctcctcgca aaccatcaac atcgggccgt
     6361 ccaccggtgg cggcgacggc ttcgtcggct tcaccgacct gtcgggcgtc agcagcgtga
     6421 cctacaacgc gaccaacgac atcgtcgcga tcgacgacgt tcgctacggc gcgctgtcgg
     6481 ccgtgccgga acccgccagc ttcgcgctgt tcggcgccgg cctgctgggc ctgggcctgg
     6541 cgcgtcgccg ccgcggctga acgcccggcc gggcgcccgc gcccgctccc gacgaggccc
     6601 gctccggcgg gccttttcca tgggcgcgac gcccgcgcgt cagggcttcg cgtcgacgaa
     6661 gcgctgctgc gcctccgagt agcgcgcccc ctcgatgcgg atcgcctccg ccgcggaatc
     6721 gatctcctgc acgtcggacg gcgtcagctc gagatcggcg gcggcaaggt tctcctccag
     6781 ccggttcagc ttggtcgtgc ccgggatcgg cacgatccac ggcttgcgcg cgaggaccca
     6841 cgccagcgcc acctgggccg gcgtggcgcc caggcgcgaa gcggtcgcct tcagcagctc
     6901 gaccagcgcc gcgttggcct tgcgcgcctc gggcgtgaag cgcggcagcg aggcgcggaa
     6961 gtcgctcgcc tgcaacggcg tcgtctcgtc catcttgccg gtgaggtaac cgcgcccgag
     7021 cgggctgaac ggcacgaggc cgatgcccag ttcctccagc acaggcagga tggtgccctc
     7081 caggtcgcgc agccacagcg agtattcgct ctgcaaggcc gccaccggtt gcacggcgtg
     7141 ggcgcggcgg atggtcgcgg catcggactc cgacagcccg aaatggcgga ccttgccctc
     7201 gcggatcagg tccttcacgg tgccggcgac gtcctcgacg ggcaccttcg ggtcgggccg
     7261 gtgctggtag aagaggtcga tcgcgtccgt gcgcaggcgc ttgagggagg cctcgcagac
     7321 cgcgcggatg cgctcgggcc ggctgtcgac gcctggcatc ctgtcgacat cgaagccgaa
     7381 cttggtcgcg atgacgacac gctcgcgcac cggcgccagc gcctcgccca gcagctcctc
     7441 gttcgtgtaa gggccgtaga tctcggcggt gtcgaagaag gtgacgccca ggtcgaccgc
     7501 ctgtcgcagc aacgcgacca tttcggcgcg atcgggcatc gggaaatagg cgtgggtcat
     7561 gcccatgcaa cccaggccga gggccgagac ctgcaggccg ctgcggccga gggaacgcgt
     7621 cttcatgcga ggctcctgtc gtcgtgggac gggcgcgccg ggcgtccgcc gaaggcgtcg
     7681 agggtggacg aaaaagggaa agcccgcgcg tggcgggctt ctccttccgg acatcgcgcg
     7741 aaaggacgcg acgacggtgc tgctgcgagc acagggtgac gggttcccct cgttccgccc
     7801 aatgttgcgg ccggttgcgt gcttgcgatg gaagaaatta tgggcagcgg ccagggcgtg
     7861 cgatgtagga cgatctgccg ttttcgtgcc tgccgcgtga cggttcgatg acgaaggtct
     7921 cacgccgccg gcgcgcggtg ctcgcgcagc gccgcgatcg cgcgcgcgta gtctccggag
     7981 ccgaagacgg ccgagccggc gaccagcgca tccgctcccg cctcgaccgc ctgccacgcg
     8041 ttgccgccgt tctgcccgcc gtcgacctcg atgtacggat tgagcccgcg cgcgtcgcac
     8101 atgcggcgca gcgcggtgat cttgggcagc gtctcgggca ggaagcgctg gccgccgaag
     8161 cccggattca ccgtcatcac gaggacgacg tcgacgagat gcagcacgtg ctcgacgaag
     8221 gccaccggac tcgcgggatc gagcaccacg ccggccttgc gaccctgctc ccggatgcgc
     8281 gagagcacgc ggtgcaggtg gatcgtcgag gccggctcgc tctgcaccag caggtggtcg
     8341 gccccggccg cgatgaagtc gtcgatcatg cgctcgggct cgacgatcat caggtgcacg
     8401 ttcagcggct tggtggtcgc gcgccggatc gcctcgacga ccaccggccc gatcgtgatg
     8461 ttgggaacga agcgcccgtc catcacgtcg acgtggatcc agtcggcgcc ggcggcatcg
     8521 accgcgcgca cctcgtcggc gaggcggccg aagtccgcgg acaggatgga cggcgcgacg
     8581 accagcgggt tcatcgtcag gccgcggcga gttcgcgcac cttgcgctcg aggccggcga
     8641 ccagcgcgtt ccatgacttc acgaacgcgt ccgcgccttc gcgctgcagg cgcagcgcca
     8701 gcgcctcgag gtcgacgccc tcgcgccgga actcgtcgag caccgccgcc gcgtggccgc
     8761 cgtcctcggg cagcggcgcg ccgaccacgc cccgctcggc gaaggcctcg agcgtcttgt
     8821 cgggcatcgt gtcgatcgtg tcgggcgcgg ccaggccctc gacgtacagc gtcggcgagg
     8881 cgtccttgtc cttggtgccg gtgctcgcga acagcaggcg ctgcggatgc gcgcccgcct
     8941 cggccaggcg ctgccagcgg cccgatgcga gtacctcgcg gtaggcgcgg tagcagtcca
     9001 tcccgatcgc gacgccgagc cggttgcggt actgggcggc cacctggtcc tgcaccgcga
     9061 cgtcccagcg gctgacgaac agcgaggcca cggactcgac caccgcggac ttgccgtcgt
     9121 cgagccggcg ctcgaggccg cgcatgtagg cgtcggcggc cgccaggtac tggtcgcgcg
     9181 agaacagcag ggtcacgttg accggcacgc ccttgtaggt cgattcctcg atggccgcga
     9241 ggccctcggg cgtgccgggg atcttgatga acaggttctc gcgcgcggcc tgcgcgtgca
     9301 ggctggccgc ggcctggatg ctgccgcgcg tgtcgcgggc gagcgtcggc gagagctcca
     9361 gcgacaccca gccgtcgatg cccccggtgg cttcccatgt cggccggaac aggtcggcgg
     9421 cggcgcgcag gtcctcgagc gcgagctgga agaagagttc ctccgtcgag cggccgtcgc
     9481 gcgcgcagcg ggcgatcgcg tcgtcgtagg cgtggccctg gcccatcgcc ttctcgaaga
     9541 tcgtcgggtt cgaggtcagg ccggtgatcg agaactcgtc gatcagccgc cgcaggtggc
     9601 cgctgtgcag gccttcgcgg ctgatgttgt cgatccacag gctctgtccg aggtcatgca
     9661 gctgtcgggt gattcggttc atggcgggct ccaggtgctc cggccagggc ggcaatattc
     9721 atgcgcgcaa gctcgccgat gcgctcgacg acgacgtcgg gcagcggacg gcgggcgagc
     9781 tcggaggggt cgtccgcgta gtgcccctgg cgcgggcaga cggtcgtcac gccgtcgccc
     9841 caggcgtcct tgacctgggc gaggatgcgc cgcttgtcgt cgaccagcac gtagcggcgc
     9901 gccggatagg tgcgtcgcac ttcgtcgagc atgcgctcct tgtggacgta gaccagcacg
     9961 cgtccgcgca cggcgtccca caggcccgag cgctcgatct tgcgcggctg gaagaccacg
    10021 tcgccgtccg acaggatcac cgtcgggccg tggcgggcga gcgtctcgag cagctcgaac
    10081 acgccggggt agacgcggtc gcggaacggg tagtcgacga ggaagcccga catccgcagc
    10141 agcgcctggg cgtcggcgcc gtggccttcc acctcgtgcc ggtagcgctg cagcgcgccg
    10201 aggtagtcga cgtagccgag ctcgtcgcgc agttgttcga acagcgccca gtagcggtcg
    10261 gcgcaggcgg cgccgaaggt cgccgcgagg tgctcgcgca ggtcagcgac gatgcggtcg
    10321 ttgtccagca gggtgttgtc gacatcgagc aggaagacga cgtcatcggc agggacggtc
    10381 acggcggcgc gctttcgtgg ggggtggacg ggccgcggca cggcgccgcg tgcgggccgg
    10441 ctgattgccg gagcctcgcc gccgg
//
LOCUS       DASVLN010000530         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_402902, whole genome shotgun sequence.
ACCESSION   DASVLN010000530 DASVLN010000000
VERSION     DASVLN010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402902"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2223
                     /locus_tag="VF453_12885"
     CDS             <1..>2223
                     /locus_tag="VF453_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688599.1"
                     /translation="LSPPLEARQAPAPAPAPLAARLPRGTMFMYGREDDLARLAAALH
                     ERRFVTLTGIGGVGKSHLAQHLLHERASAYEQGAVWVELAQLFAPELVIDTVAAGVGI
                     AVGGIDPLGDLVAGLRGRELLLGIDNAEHLADEVARVVGAIAAGAPRVTLLVTSQAPL
                     RLPREHVLRLAPLALPPADASVAQAMRHGAVELFVRRAQETDMRFSLQAHNVAAVIDI
                     CRRVDGLPLALELAAARTPLLRLAGLTAMLSDPLRLLTATTKRPHDPRHRALRITLEW
                     SHDLLGPREKKAFRRLAAFVGSCSLALACEVLRDAAPEPRGDPAEGIPDTWAALEALN
                     ELTERSLVEADESDPPRYRLLETSRAFANERLEAAGESEAMRGRHARAVAASFAAIDE
                     AIASGELTRDHAVDQVWPDIANARAAIEWSLVNEPPLALTIVPVIEDLFKSILFPDVR
                     RMWNATLPLLDDRLPPRLRARWALGYAHFWTNRDLALTLEWSGIAYDLYSRDDSGADN
                     LPRTLRALYLRVTATARSGGDTTADLARLRAIDCSRRPVAMRYLQVFAEGVLHALKGN
                     FPEALAYYGRALPLAQMVGDRELYFALMLNTIDMEIAIGRLDDAIRKGQELVAGLRGT
                     RFLELTTIALNNLAAALIAKGSLAEARQVIEQGLPMADDHSLTAEWADHLAWFSAQEG
                     RYYDAAHLLGYGDLRYEQRGSIRSNIEANAARQAEALARQAFGDAAFESERAFGRTLP
                     D"
BASE COUNT          292 a          843 c          799 g          289 t
ORIGIN      
        1 ctgtcgccgc ccctggaggc gcgccaagcg ccggcgccgg cgccggcgcc gctggcggca
       61 cggctgccgc gcgggacgat gttcatgtat ggccgcgagg acgacctcgc gcgcctggcg
      121 gccgccctgc acgagcgtcg cttcgtcacg ctgacgggca tcggcggcgt gggcaagtcg
      181 cacctcgccc agcacctgct gcacgagcgg gcatccgcgt acgaacaggg cgccgtgtgg
      241 gtggagctgg cccagctgtt cgcgcccgag ctggtgatcg acaccgtcgc ggccggcgtc
      301 ggcatcgcgg tgggcgggat cgacccgctg ggcgacctgg tcgcggggct gcgcgggcgc
      361 gagctgctgc tgggcatcga caacgccgag cacctggccg acgaggtcgc tcgcgtcgtg
      421 ggtgccatcg ccgccggcgc cccgcgggtg acgctgctgg tcacgagcca ggcaccgctg
      481 cgcctgccgc gcgagcacgt gctgcggctg gcgccgctcg ccctgccccc ggcggacgcc
      541 tcggtcgcgc aagccatgcg ccacggcgcc gtcgagctgt tcgtgcgccg cgcgcaggag
      601 acggacatgc gcttctcgct gcaggcgcac aacgtggccg cggtgatcga catctgccgc
      661 cgcgtcgacg gcctgccgct cgccctggag ctggcggcgg cgcgcacccc gctgctgcgg
      721 ctggccggcc tgacggccat gctgtcggat ccgctgcgcc tgctgacggc cacgaccaag
      781 cgcccgcacg atccgcgcca tcgcgcgctg cgcatcacgc tggaatggag ccacgacctg
      841 ctcggcccgc gcgagaagaa ggccttccgc cgcctcgccg cgttcgtggg cagttgctcg
      901 ctggcgctcg catgcgaggt gttgcgcgac gccgcgccgg agccccgggg cgatcccgcc
      961 gaaggcatcc ccgacacctg ggcggcgctg gaggcgctca acgagctgac ggagcgctcg
     1021 ctggtcgagg ccgacgaaag cgacccgccg cgctaccggc tgctcgagac ctcgcgcgcg
     1081 ttcgccaacg aacgcctgga ggccgccggc gagtcggagg cgatgcgcgg gcgccatgcg
     1141 cgcgccgtcg ccgccagctt cgccgcgatc gacgaggcga tcgcatcggg cgagctcacc
     1201 cgcgaccacg ccgtcgacca ggtctggccc gacatcgcca atgcgcgggc ggcgatcgag
     1261 tggtcgctgg tcaacgaacc gccgctcgcg ctgaccatcg tgccggtgat cgaggacctg
     1321 ttcaagtcga tcctgttccc cgacgtgcgg cggatgtgga acgccacgct gccgctgctg
     1381 gacgaccgcc tgccgccgcg gctgcgcgcg cgctgggcgc tcggctacgc ccacttctgg
     1441 accaaccgcg acctcgcgct gacgctggag tggtccggca tcgcctacga cctgtacagc
     1501 cgcgacgaca gcggcgccga caacctgccg cgcacgctgc gcgcgctgta cctgcgcgtc
     1561 acggcgacgg cgcgctccgg cggcgacacc accgccgacc tggcgcgcct gcgcgcgatc
     1621 gactgcagcc ggcgcccggt cgcgatgcgc tacctgcagg tcttcgcgga gggcgtcctg
     1681 cacgcgctga agggcaactt ccccgaggcg ctcgcctatt acggacgcgc cctgccgctg
     1741 gcgcagatgg tcggcgaccg cgagctctac ttcgcgctga tgctcaacac catcgacatg
     1801 gagatcgcca tcggccgcct ggacgacgcg atccgcaagg gccaggagct ggtcgccggc
     1861 ctgcgcggga cgcgcttcct ggagctgacg acgatcgcgc tgaacaacct cgccgcggcc
     1921 ctgatcgcga agggctcgct ggccgaggcg cgtcaggtca tcgagcaggg cctgccgatg
     1981 gccgacgacc actcgctgac cgcggagtgg gcggaccacc tcgcctggtt ctcggcccag
     2041 gaaggccgct actacgacgc cgcgcacctg ctcggctacg gcgacctgcg ctacgaacag
     2101 cgcggatcga tccgctccaa catcgaggcg aacgccgcca ggcaggccga ggcgctggcc
     2161 cgccaggcgt tcggcgacgc ggccttcgag agcgagcgcg ccttcggtcg cacgctgccg
     2221 gac
//
LOCUS       DASVLN010000531         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_188653, whole genome shotgun sequence.
ACCESSION   DASVLN010000531 DASVLN010000000
VERSION     DASVLN010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188653"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1861)
                     /locus_tag="VF453_12890"
     CDS             complement(182..1861)
                     /locus_tag="VF453_12890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7688600.1"
                     /translation="MSANVVDDAWHDLSHAQAGLAEMRQLQALLVVAEMASRERGPAN
                     GVLGDELPGDPAKRQRLLLARQKTDSAFDALRRMPGTASDATERVQPMILRARQQLER
                     ARLAVDAEARKPRAARTPAEIREAVNQMIEVVDDLTPATIRLTNDASATFPVAANSLV
                     AARQAAMLREYAGRLGSLFTGSLTLRRRLSTQERAAIDQMRGRIEQLRQQLSAFAAAA
                     GSLPPAQAAIDDMQARYFGRAIPFVEAQTDIGMRDAQYDVDTAQFAARYVPDMDPIIA
                     VRDVLMADATDHARRGLAHARDAALLTASGAVLTLGLLALTLWLLHRRVIRPLARTTA
                     LIVTIAQGQLDVHVPTPRHRDEVADMLGAIGVLRDNSVARRAAEEAIRQMAYYDRLTG
                     LPNRRLLEDRMEQALASARRRGAKVSVLFIDLDKFKQVNDQQGHDAGDWLLQQIAGRM
                     RAALRESDTAARIGGDEFVVLLPDTARVEDAVVAAEKIRREMERPFAMADGVTLQISC
                     SIGVAMYPDQAEASQDLLRFGDEAMFRAKKHGRNAIEVFSGGDCAPHAQDI"
     gene            complement(2166..2723)
                     /locus_tag="VF453_12895"
     CDS             complement(2166..2723)
                     /locus_tag="VF453_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688601.1"
                     /translation="MLRLLATLALLAGAAAGAASASTSATFATEDSTAGWATVKESLA
                     QALVHAGDAEDRRQLDDTIRDDGLPRNDRQFLRARPVQLAVAGHPGLFVRPATQPHAN
                     AFYGAHTFRFWFVDAGGRLLFTSTADKVAVLPSVHEGMHDLRVSQCRGGECFETDEVF
                     ERGRYRDGACVTTSIASGQASAGCR"
     gene            complement(2781..>3270)
                     /locus_tag="VF453_12900"
     CDS             complement(2781..>3270)
                     /locus_tag="VF453_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7688602.1"
                     /translation="GLVRLMAREVAQPQPGSTALVGELAGALFTLALRAHLAAHPAHD
                     GVLGLMRHDRLAPALKAMLDEPAAPWTVESLADRCHLSRARFARLFAEHARTAPLAML
                     TRLRMELAARLLAQGGMDTAAVGEAVGYRSEAAFNRAFARHAGVPPGRFRRQAQGEAL
                     GA"
BASE COUNT          449 a         1150 c         1223 g          448 t
ORIGIN      
        1 cgtcgggcgc ggcgcctcgg cgaccgccgg gctcgcggcg acgccggtga gcgccatgaa
       61 caggaacggg agcatctgac ggggacgggg cgggttgccg cgagtcgggg gcaccattct
      121 cgccgcaggc gtccggcagc ctgggcgccg caagcggcga ccgtgcgcgg gcccgggcct
      181 gtcagatatc ttgtgcgtgg ggcgcgcagt cgccgccgga gaacacctcg atcgcattac
      241 gcccgtgctt cttggcgcgg aacatcgcct cgtcgccgaa gcgcaggagg tcttgcgacg
      301 cttcggcctg gtcggggtac atcgccacgc cgatgctgca ggagatctgc agcgtgacgc
      361 cgtccgccat ggcgaagggc cgctccatct cccgccggat cttctcggcc gcgaccacgg
      421 cgtcctccac ccgggcggtg tcaggcagca gcaccacgaa ctcgtcgccg ccgatgcgcg
      481 cggcggtgtc ggactcgcga agggcggcgc gcatgcggcc ggcgatctgc tgcagcaacc
      541 agtcgcccgc gtcgtggcct tgctggtcgt tgacctgctt gaacttgtcc aggtcgatga
      601 acagcaccga caccttggcg ccgcgacggc gcgcgctggc cagcgcctgc tccatgcgat
      661 cctcgagcag gcggcggttc ggaaggccgg tcaggcggtc gtagtaggcc atctggcgga
      721 tcgcctcctc ggccgcacgg cgcgcgacgc tgttgtcgcg cagcacgccg atggcgccca
      781 gcatgtcagc cacttcgtcg cggtggcgcg gcgtcggcac atggacgtcg agctgcccct
      841 gcgcgatggt cacgatgagc gcggtcgtcc gcgccaaggg gcggatcacg cgccggtgca
      901 gcagccacaa ggtcagcgcg agcaggccga gcgtcagcac ggcgccggag gcggtcagca
      961 gcgcggcgtc gcgagcgtgc gcgagccccc gcctggcgtg atccgtggcg tccgccatca
     1021 ggacgtcgcg cacggcgatg atcggatcca tgtccggcac gtagcgcgcg gcgaactgcg
     1081 cggtgtcgac gtcgtactgg gcgtcgcgca tgccgatgtc ggtctgcgcc tccacgaacg
     1141 ggatcgcacg gccgaagtag cgggcctgca tgtcgtcgat ggcggcctgc gcgggcggga
     1201 gcgatccggc ggcggccgcg aatgccgaga gctgctggcg cagctgttcg atgcgtccgc
     1261 gcatctggtc gatggccgcg cgctcttgcg tgctcagccg ccgccgcagg gtcagcgatc
     1321 cggtgaacag cgagccgagg cgtccggcgt actcgcgcaa catggccgcc tgccgcgccg
     1381 cgaccagcga attggcggcg acgggaaagg tggccgacgc atcgttcgtc aggcggatcg
     1441 tcgccggcgt caggtcgtcg acgacctcga tcatctggtt gacggcctcg cggatctcgg
     1501 ccggcgtgcg cgcggcgcgt ggtttgcggg cttcggcatc gactgccagc cgagcgcgtt
     1561 cgagctgctg tcgggcgcgc aggatcatcg gctgcacgcg ctccgtggcg tcggaggcgg
     1621 tgcccggcat gcggcgcagg gcgtcgaacg ccgaatcggt cttctggcgc gccagcagca
     1681 ggcgctggcg cttggcgggg tcgccgggca gctcgtcgcc cagcacgccg tttgcggggc
     1741 cgcgttcccg cgacgccatc tccgcgacca cgagcagggc ctgcagctgg cgcatctcgg
     1801 cgaggccggc ctgcgcatgg ctcaagtcgt gccacgcgtc gtcaacgacg ttggcgctca
     1861 tggcgacgac ggtggcggac agcgcgcagc tcaggacaag aaagagacgt cgaagtcgca
     1921 cgtaccgcta ctcaagactg tggatgccac ggaaagacgc ggcaggcccc gcctcgaagg
     1981 ccgccttccg ctcgcgagca tccgcgttcg tcgcgagctc atcataggcg ccgaacccgc
     2041 ggattcaacg cagcgacctc aacgcggcgc acgcattcgt gacgtttgac gccgacacgt
     2101 tttgcaggcg ctcgccggct tctgcgtgcg ccggcgcagg tctgggcggt cgccgtgccg
     2161 acgcttcagc ggcagccggc agacgcctgg ccgcttgcga tggaagtcgt gacgcacgcg
     2221 ccgtcccggt agcggccgcg ctcgaagacc tcgtcggtct cgaagcactc gccgccgcgg
     2281 cattgcgaca cgcgcaggtc gtgcatgccc tcgtggacgg aaggcagcac ggccaccttg
     2341 tccgcggtcg acgtgaacag cagccgcccg cccgcgtcga cgaaccagaa gcggaacgtg
     2401 tgggcgccgt agaaggcgtt cgcgtgcggc tgcgtggcgg gtcggacgaa caggccggga
     2461 tgtccggcga cggcgagctg cacgggccgg gcgcgcagga actggcggtc gttgcgcggc
     2521 aggccgtcgt cgcggatcgt gtcgtccagc tggcgccggt cctcggcatc gccggcgtgc
     2581 acgagcgcct gtgccagcga ttccttgacg gtcgcccagc cggccgtcga atcctccgtg
     2641 gcgaaggtgg cggacgtgga ggcggacgcc gcgccggcgg ctgcgcccgc gagcagcgcc
     2701 agggtggcga gcagacggag catgcacacc tcacttgccc gccagcgtgc cgcacgagtc
     2761 gcaggccggt cgcgcggtcg tcatgcgccg agtgcctcgc cttgcgcctg ccgccggaac
     2821 cgccccggcg gcacgccggc atgccgcgcg aacgcccggt tgaacgcggc ttcggaccga
     2881 taccccaccg cctcgccgac ggccgccgtg tccatgccgc cctgcgccag caagcgcgcc
     2941 gccagctcca tccgcaatcg cgtgagcatc gccagcggcg ccgtccgcgc atgctcggcg
     3001 aacaggcgcg cgaagcgggc gcgcgagagg tggcagcggt cggcgagcga ttcgaccgtc
     3061 cacggcgcgg ccggctcgtc gagcatcgcc ttgagcgcgg gcgcgagccg atcgtggcgc
     3121 atcagcccga gcacgccgtc gtgcgcggga tgcgcggcca ggtgcgcgcg caaggcgagc
     3181 gtgaagagcg cgccggcgag ttcgccgacg agggccgtcg agccgggctg cggctgggcg
     3241 acctcccggg ccatcagccg gacgaggccc
//
LOCUS       DASVLN010000532         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_458315, whole genome shotgun sequence.
ACCESSION   DASVLN010000532 DASVLN010000000
VERSION     DASVLN010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458315"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1554)
                     /locus_tag="VF453_12905"
     CDS             complement(166..1554)
                     /locus_tag="VF453_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX7688603.1"
                     /translation="MHVQASAFKAYDIRGIVGESLDEAFAEHLGRAFGTEALRLGERA
                     VAVGRDGRLSGPALAAALVRGLASTGLDVVDLGPVTTPMLYYVAATRGAHGCRSGIQV
                     TGSHNPKDWNGFKMVLAGKAIYGEDIQGLRRRMEAEDYVIGEGRAGRMDVLAEYTHRI
                     ASDCKLARGMRIVVDSGNGIPGASAPGILRALGCEVIDLYSRVDGNFPNHHPDPSKPE
                     NLADLIRTVHATDAELGLAFDGDGDRLGVVTRGGNVIYPDRQLMLFARDILSRVKGGQ
                     VIYDVKCSQRLPVAIREAGGEPVMWKTGHSLVKAKLKETGAPIAGEMSGHIFFSERWY
                     GFDDATYTAARLLEILSRSADPSAVLDELPTSFSTPELNVACAEGEPPQVIARLLAEA
                     TFPGAEILTIDGLRAEYPDGFGLVRASNTTPVLVLRFEGHTPEALERIQGDFLAALRA
                     VKPDAQVGSAAH"
     gene            1708..>2670
                     /locus_tag="VF453_12910"
     CDS             1708..>2670
                     /locus_tag="VF453_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7688604.1"
                     /translation="MPDDRAPTRDARLRQSWMNWLGLEPAAAGSAWARIGWTFVFCVG
                     IAAGYTLLFFTTNARSLADWLSPRLWLVMYARNLGISLVIGYLIQGLFTLVSKSLGPA
                     RLRALPAWGRLALFTLLPLVGTAIGWPLGVALVFLDARILARTPPAEVFGSLVLSLMI
                     SSLFLLYYTVRLREALARRSAADAQLRLLQGQMEPHFLFNTLANVVSLIDADAPRAKR
                     MLETFTDYLRASLGSLRRDQSTLDEELELATRFLLVMQARMGERLRFEIDVEPALRGV
                     PLPPLLLQPLVENAVKHGLEPQVDGGTVRVSARRAEVDGRPVLRL"
BASE COUNT          330 a          982 c          959 g          399 t
ORIGIN      
        1 ccaggcgctc gcccatcgcg acgcggtagc cgggctcctt gcgcccgcgc caccagtagc
       61 gggcgagcat cgccggcagc agcagccgca gcgcccaggc gtaggccgcg agcgcgaggc
      121 gctgcctgga cgtcatcggc acggcgcggc ggatgcgcgg gacgatcaat gggccgccga
      181 gcccacctgc gcatccggct tgaccgcccg gagcgcggcg aggaaatcgc cctggatgcg
      241 ctccagcgcc tcgggcgtgt ggccctcgaa gcgcagcacc agcaccggcg tcgtgttgga
      301 cgcgcgcacc aggccgaagc cgtcggggta ttcggcccgc aggccgtcga tcgtgaggat
      361 ctcggcgccc gggaacgtcg cctcggccag caggcgcgcg atgacctggg gcggctcgcc
      421 ctcggcgcac gccacgttga gctcgggcgt gctgaagctg gtcggcagct cgtcgagcac
      481 cgcgctcggg tcggcgctgc gcgagaggat ctccagcagc cgcgcggccg tgtaggtcgc
      541 gtcgtcgaag ccgtaccagc gctcgctgaa gaagatgtgg ccgctcatct cgccggcgat
      601 cggcgcgccg gtctccttca acttcgcctt gaccagcgag tggcccgtct tccacatcac
      661 cggctcgccg ccggcctcgc ggatggccac cggcaggcgc tgcgagcact tgacgtcgta
      721 gatgacctgg ccgcccttga cgcgcgagag gatgtcgcgc gcgaacagca tcagctggcg
      781 atcggggtag atcacgttgc cgccgcgcgt gacgacgccc aggcggtcgc cgtcgccgtc
      841 gaaggccagg cccagctcgg cgtccgtcgc gtgcacggtg cggatgagat cggcgaggtt
      901 ctccggcttg gacgggtcgg gatggtggtt gggaaagttg ccgtcgacgc ggctgtagag
      961 gtcgatcacc tcgcagccga gcgcgcgcaa gatgcccggg gcggacgcgc ccgggatgcc
     1021 gttgcccgag tcgacgacga tgcgcatgcc gcgcgcgagc ttgcagtcgc tcgcgatgcg
     1081 gtgggtgtac tcggccagca cgtccatcct gccggcgcgg ccctcgccga tcacgtagtc
     1141 ctcggcctcc atgcggcggc gaaggccctg gatgtcctcg ccgtagatcg ccttgccggc
     1201 gagcaccatc ttgaagccgt tccagtcctt cgggttgtgg ctgccggtga cctggatgcc
     1261 cgagcggcag ccgtgcgcgc cgcgggtggc ggcgacgtag tacagcatcg gcgtcgtcac
     1321 cgggccgagg tcgacgacat ccagcccggt cgacgccagg ccgcggacga gcgccgccgc
     1381 cagcgccggg ccggacagcc ggccgtcgcg gccgacggcg accgccctct cgcccaggcg
     1441 caacgcctcg gtgccgaacg cgcggccgag atgttctgcg aaggcttcgt cgagcgactc
     1501 gccgacgatg ccgcggatgt cgtaggcctt gaaggccgat gcttggacgt gcatgggggt
     1561 ttctcgttcg tgcgggcccg gccgggccgc ggttggcggc gattctacgg gccgaccgcc
     1621 ggcgccgcgc gctggccgtc cgtcgcgccg gccgtcgatc cgtcgcgacc cgcgaatgcc
     1681 tcgccgccgc ctgcctacac tggcgccatg cccgacgacc gcgccccgac ccgcgacgcc
     1741 cgcctgcgcc agtcctggat gaactggctg ggcctcgaac ccgccgcggc cggctcggcc
     1801 tgggcgcgca tcggctggac cttcgtcttc tgcgtcggca tcgcggccgg ctacacgctg
     1861 ctgttcttca cgacgaacgc gcgctcgctc gcggactggc tctcgccgcg gctgtggctc
     1921 gtcatgtatg cccgcaacct gggcatctcg ctggtcatcg gctacctcat ccagggcctg
     1981 ttcacgctgg tgtccaagag cctcgggcca gcgcggctgc gcgccctgcc cgcctggggc
     2041 cggctcgccc tgttcacgct gctgccgctg gtgggcacgg cgatcggctg gccgctcggc
     2101 gtggcgctgg tgttcctcga tgcccgcatc ctcgcgcgca cgccgcccgc ggaggtgttc
     2161 ggcagcctcg tgctctcgct gatgatcagc agcctgttcc tgctctacta cacggtgcgc
     2221 ctgcgcgagg cgctcgcccg gcgcagcgcc gccgacgccc agctgcgcct gctgcagggc
     2281 cagatggagc cgcacttcct gttcaatacg ctggccaacg tcgtgagcct catcgacgcc
     2341 gacgcgccgc gcgccaagcg gatgctggag accttcaccg actacctgcg cgcctcgctc
     2401 ggcagcctgc ggcgcgacca gtccacgctg gacgaggagc tcgagctcgc cacgcgcttc
     2461 ctgctcgtga tgcaggcgcg catgggcgag cggctgcgct tcgagatcga cgtcgagccg
     2521 gcgctgcgcg gcgtgccgct gccgccgctg ctgctgcagc cgctcgtgga gaacgccgtc
     2581 aagcacggcc tggagccgca ggtcgacggc ggcaccgtgc gcgtgagcgc gcgccgcgcg
     2641 gaggtcgacg gccggcccgt gctgcgactc
//
LOCUS       DASVLN010000533         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_402995, whole genome shotgun sequence.
ACCESSION   DASVLN010000533 DASVLN010000000
VERSION     DASVLN010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402995"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VF453_12915"
     CDS             <1..346
                     /locus_tag="VF453_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688605.1"
                     /translation="GPAVAAAEPSRAADAEAPPDAPARPRSKGPEDSIPRLSHASLAH
                     EGRIRPAGVAYFTLRRDSAGAASRDVADASPPTFPAGAREADRRRWRRAPAWAALRIA
                     PWQAAPSANSFA"
     gene            310..1425
                     /gene="aroC"
                     /locus_tag="VF453_12920"
     CDS             310..1425
                     /gene="aroC"
                     /locus_tag="VF453_12920"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX7688606.1"
                     /translation="MAGSTFGELFRVTNFGESHGVAIGCVIDGCPPGLAISEADIQVE
                     LDRRRPGTSRHVTQRNEPDAVEILSGVYEGLTTGTPIALLIRNTDQRSKDYGNILDTF
                     RPGHADYTYWRKYGLRDPRGGGRSSARLTAPMVAAGAVARKWLKERHGVSFVGWMSQL
                     GDIAIPFVDEAEIARNPFFAPNAQIVPALEACMDDLRKAGDSVGARIECVARGMPAGL
                     GQPLFDKLDADIAYAMMGINAVKGVEIGAGFASVAQRGTEHGDELTPAGFVGNNAGGV
                     LGGISTGQDLRVSIAIKPTSSIRTPRRSIDRAGAPATVETFGRHDPCVGIRATPIVEA
                     MLALVVMDHVLMHRAQCGDVELPVPPIAASAGAAGAP"
     gene            1577..>2423
                     /locus_tag="VF453_12925"
     CDS             1577..>2423
                     /locus_tag="VF453_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7688607.1"
                     /translation="MRKRWLIGVAAVAAIAGGAVALKTGKAGLMGFGRAAVAAEGAAS
                     APAEPPLDFTPAEAARPVLMALPQVVEFSGPLVAPNTATVRAKATGTLLALDVQEGQR
                     VKAGQPLGRIDVSELSSRVAERAANLASAQATLAQAQRTHDSNVGLANDKFISPIALE
                     NSRTQLDGAKAQVAAAQAQLESARVALREASLVAPIEGIVAKRYVVAGEKVTTEQQVV
                     DIVDLRQLELAGTVGTQDVPRLAAGMPVELRVEGVDAPVHGALARIAPQAEAGTRSIG
                     VAVRLA"
BASE COUNT          341 a          917 c          870 g          295 t
ORIGIN      
        1 aggtccggcc gtcgcggccg ccgagccgtc gcgggccgcg gacgccgaag ccccgccgga
       61 tgcgcctgcc cgaccgcgct cgaaggggcc ggaagacagc attccgcgac tgtcccacgc
      121 cagccttgca catgagggcc ggataaggcc cgcgggcgtg gcctatttca cgctgcggcg
      181 cgatagcgcg ggcgccgcgt cgcgagatgt cgccgatgcg tcgccgccga cgtttcccgc
      241 gggcgcgcgc gaagcggatc gccgccgatg gcgaagggcg cccgcatggg ccgcccttag
      301 aatcgcgcca tggcaggcag caccttcggc gaactctttc gcgtgaccaa tttcggcgag
      361 tcgcacggcg tggcgatcgg ctgcgtgatc gacggctgcc ccccgggcct cgccatctcg
      421 gaggccgaca tccaggtcga gctcgaccga cgccgccccg gcacctcgcg ccacgtcacg
      481 cagcgcaacg agccggacgc cgtggagata ctctcagggg tatatgaggg cctgaccacc
      541 ggcaccccga tcgcactcct gatccgcaac acggaccagc gcagcaagga ctacggcaac
      601 atcctcgaca ccttccgccc cggccacgcc gactacacct actggcgcaa gtacgggctg
      661 cgcgacccgc gcggcggcgg ccgttcctcg gcccggctga cggcgcccat ggtcgcggcc
      721 ggcgcggtcg cccgcaagtg gctgaaggag cgccacggcg tgtcgttcgt cggctggatg
      781 agccagctcg gcgacatcgc gatccccttc gtcgacgagg ccgagatcgc gcgcaacccg
      841 ttcttcgcgc cgaacgcgca gatcgtgccg gcgctcgagg cctgcatgga cgacctgcgc
      901 aaggcgggcg actccgtcgg cgcgcgcatc gagtgcgtcg cccgcggcat gcccgccggg
      961 ctcggccagc cgctgttcga caagctcgac gccgacatcg cctacgcgat gatgggcatc
     1021 aacgcggtca agggcgtcga gatcggcgcc ggcttcgcga gcgtcgccca gcgcggcacc
     1081 gagcacggcg acgagctcac gcccgccggc ttcgtcggca acaacgccgg cggcgtgctg
     1141 ggcgggatct cgaccggcca ggacctgcgc gtctcgatcg cgatcaagcc caccagctcg
     1201 atccgcacgc cgcgccgctc gatcgaccgc gccggcgcgc cggcgacggt ggagacgttc
     1261 ggccgccacg acccctgcgt cggcatccgc gcgacgccga tcgtcgaggc catgctcgcc
     1321 ctggtcgtga tggaccacgt gctgatgcac cgcgcgcaat gcggcgacgt cgagctgccg
     1381 gtgccgccga tcgccgcgtc cgcgggggcc gccggcgcgc cctgagcgcg cccgctccga
     1441 ctcgtccggg cggactgcag ttcgtccccg ctcgtccgca cttcgtcgca atcgccgcct
     1501 gtcgcccgcg cgagggatcc acactccgcg cattccggag gacactccgg gccacaacga
     1561 cgacgaggaa agagcaatgc gcaaacgctg gctgataggg gtggcggcgg tggcggcgat
     1621 cgccgggggc gcggtcgcgc tgaagaccgg caaggcgggg ctgatgggct tcggccgcgc
     1681 cgcggtcgcc gccgagggcg ccgccagcgc gcccgccgag ccgccgctgg acttcacgcc
     1741 cgccgaggcc gccaggccgg tgctgatggc gctgccccag gtcgtcgagt tctcggggcc
     1801 gctcgtcgcc cccaacaccg cgaccgtgcg cgccaaggcc accggcacgc tgctggcgct
     1861 ggacgtgcag gaggggcagc gcgtgaaggc gggccagccg ctggggcgca tcgacgtgag
     1921 cgagctctcg agccgcgtcg ccgagcgcgc cgccaatctc gcgtcggcgc aggcgacgct
     1981 cgcgcaggcg cagcgcacgc acgactccaa cgtcggcctg gccaacgaca agttcatctc
     2041 gccgatcgcc ctcgagaact cgcgcaccca gctcgatggc gcgaaggccc aggtggcggc
     2101 cgcgcaggcg cagctggagt cggcgcgcgt cgcgctgcgc gaggcctcgc tggtggcgcc
     2161 catcgagggc atcgtcgcca agcgctacgt cgtcgcgggc gagaaggtca cgaccgagca
     2221 acaggtcgtc gacatcgtcg acctgcgcca gctcgagctc gcgggcaccg tcggcacgca
     2281 ggacgtcccg cgcctggctg ccggcatgcc ggtggagctg cgggtggagg gcgtcgacgc
     2341 gccggtgcat ggcgcgctcg cccgcatcgc gccgcaggcc gaggccggca cgcgctcgat
     2401 cggcgtcgcg gtgcgcctgg cca
//
LOCUS       DASVLN010000534         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_512969, whole genome shotgun sequence.
ACCESSION   DASVLN010000534 DASVLN010000000
VERSION     DASVLN010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512969"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VF453_12930"
     CDS             complement(<1..782)
                     /locus_tag="VF453_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7688608.1"
                     /translation="MPKTQAITDFAPDSFAAMFEESLKSSEMRSGEVITAEVVRIDHS
                     FVVVNAGLKSEAYVPIDEFKNDQGEIEVQVGDFVAVAIDAVENGYGDTILSRDKAKRL
                     ASWLSLENALESGEFVTGTVSGKVKGGLTVLVNGIRAFLPGSLLDTRPVKDMSPFEGK
                     TMEFKVIKLDRKRNNVVLSRRAVVEASMGEERAKLLETLSEGAIVNGVVKNITEYGAF
                     VDLGGIDGLLHITDMAWRRVRHPSEVVTVGQELTAKVLKFDA"
     gene            complement(983..>2755)
                     /locus_tag="VF453_12935"
     CDS             complement(983..>2755)
                     /locus_tag="VF453_12935"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HEX7688609.1"
                     /translation="LRAREAALFLGNAGTAMRPLTAALAVLATLQGGAFQLSGVPRMH
                     ERPIGDLVDALVQLGCAVEYRGNPGFPPLAVGDGATGSLSTDEPIRVRGDVSSQFLTA
                     LLLALPLATGDEAVMIAVEGELISKPYVDITLNLLARFGIRVERDAANPYARFTLPRG
                     SRYRSPGAIHVEADASSASYFVALGALAGVDAPVRIEGVGLDSIQGDIRFVEAARAMG
                     AQVEGGPGWLEVRRGAWPLQAIALDCNHIPDAAMTLAVMALYADGATRLTNIASWRVK
                     ETDRIAAMAAELRKVGATVVEGADFIEVSPPAAWRAAEVETYDDHRMAMCLSLAAFNP
                     LAGANPARPVRILDPKCVAKTFPDYFEALFSLARAEVADIPVIAIDGPTASGKGTLAS
                     RVADALGYALLDSGALYRATALAVQRAGADPDDEAAVAALAGALDLHFEADRAFLADE
                     DVTDALRLEATGNLASRISAHPAVRRALHDLQLAFRRLPGLVADGRDMGTVVFPQAAL
                     KVFLTASVDERAARRHAQLLARGASANIDSLRAELQQRDERDRNRAVAPLRPAEDAVL
                     LDNSGMSIDACVDFVLEAWNARRM"
BASE COUNT          395 a          923 c         1043 g          394 t
ORIGIN      
        1 tcggcgtcga acttcaggac cttggccgtc agttcctggc cgaccgtcac cacttcgctc
       61 gggtgacgga cgcggcgcca ggccatgtcg gtgatgtgca gcaggccgtc gatgccgccg
      121 aggtcgacga acgcgccgta ctcggtgatg ttcttgacca cgccattgac gatcgcgcct
      181 tcggacagcg tctcgagcag cttggcgcgc tcttcgccca tcgaggcctc gaccacggca
      241 cggcgcgaca gcacgacgtt gttgcgcttg cggtcgagct tgatgacctt gaattccatg
      301 gtcttgccct cgaacggcga catgtccttg acgggacgcg tgtcgaggag cgagccgggc
      361 aggaaggcgc ggatgccgtt gacgagaacc gtcaggccgc ccttgacctt gccgctgacc
      421 gtgccggtga cgaactcgcc cgattccagc gcgttctcga gcgacagcca cgaggcgagg
      481 cgcttggcct tgtcgcgcga caggatggtg tcgccgtagc cgttctcgac ggcgtcgatc
      541 gcgaccgcca cgaagtcgcc gacctggact tcgatctcgc cctggtcgtt cttgaactcg
      601 tcgatgggca cgtacgcttc gctcttcagg ccggcgttga cgacgacgaa gctgtggtcg
      661 atgcgcacca cctcggcggt gatgacttcg ccggagcgca tttcgctgct cttgagcgat
      721 tcctcgaaca tcgcggcgaa ggaatcgggc gcgaaatcgg tgatggcttg ggtcttgggc
      781 atggtgtttc ctggggctgg cggcgcgacg cgagggaaag gttcggcgag ggccgcggca
      841 gcggtgtgca acccgggaag gtcgcggtta ggttggacat ccaccgtccc gatccggccg
      901 gggaggccgg gatcgacgga ccgcgcggcg cctgcgaggg tgcctggcgg tccagacggt
      961 gggctggact ggcgatgaaa ggtcacatgc ggcgcgcgtt ccaggcttca agcacgaagt
     1021 cgacgcaggc gtcgatgctc atgccggagt tgtcgagcaa caccgcatcc tcggccggcc
     1081 ggagcggggc gacggcgcga ttgcggtcgc gctcgtcgcg ctgctggagc tcggcgcgaa
     1141 gactgtcgat attagcagaa gcgccgcggg cgagcaattg cgcgtgccgg cgcgcggcgc
     1201 gctcgtcgac gctggcggtc aggaagacct tgagcgccgc ctgcgggaac acgacggtgc
     1261 ccatgtcgcg gccgtcggca acgaggccgg gcaggcggcg gaacgcgagc tgcaggtcgt
     1321 gcagggcgcg ccgcacggcc gggtgggcgg agatgcgcga ggcgaggttg ccggtggcct
     1381 ccaggcgcag cgcatcggtg acgtcctcgt cggcgaggaa ggcgcggtcg gcctcgaagt
     1441 gcaggtcgag cgccccggcc agtgccgcga cggcggcctc gtcgtccgga tcggcgcccg
     1501 cgcgctgcac ggcgagcgcg gtcgcgcgat agagcgcgcc ggagtcgagc agcgcgtagc
     1561 cgagcgcgtc ggccacgcgc gacgcgagcg tgcccttgcc ggacgccgtc gggccgtcga
     1621 tcgcgatgac cgggatgtcg gcgacctcgg cgcgcgcgag cgagaacagt gcctcgaagt
     1681 agtccgggaa ggtcttggcg acgcacttcg ggtcgaggat gcgcaccggc cgggccgggt
     1741 tcgcgccggc gagcgggttg aacgccgcca gcgacaggca catcgccatg cggtggtcgt
     1801 cgtaggtctc gacctcggcg gcgcgccagg cggcgggggg cgagacctcg atgaagtccg
     1861 cgccctcgac gaccgtcgcg ccgaccttgc gcagctcggc ggccatcgcg gcgatgcggt
     1921 cggtctcctt gacgcgccag ctggcgatgt tggtcagccg ggtcgcgccg tcggcgtaca
     1981 gcgccatcac ggccagcgtc atcgcggcgt cggggatgtg gttgcagtcg agcgcgatcg
     2041 cctgcagcgg ccacgcgccg cggcgtacct cgagccagcc cggcccgccc tcgacctgcg
     2101 cgcccatcgc gcgcgccgcc tcgacgaagc ggatgtcgcc ctggatcgaa tccaggccga
     2161 cgccctcgat gcgcacgggc gcgtcgacgc ccgcgagcgc ccccagcgcg acgaagtacg
     2221 aggccgacga ggcgtccgcc tcgacatgga tcgcgcccgg cgagcggtag cgcgagcccc
     2281 gcggcagcgt gaagcgcgcg taggggttcg cggcgtcgcg ctcgacgcgg atgccgaagc
     2341 gcgccagcag gttcagcgtg atgtcgacgt agggcttgga gatcagctcg ccctcgaccg
     2401 cgatcatgac ggcctcgtcc ccggtggcca gcggcagcgc gagcagcagg gcggtcagga
     2461 actggctcga gacgtcgccg cgcacgcgga tcggctcgtc ggtggacagc gagccggtcg
     2521 cgccgtcgcc gacggccagc ggcgggaagc ccgggttgcc gcggtactcg accgcgcagc
     2581 cgagctgcac cagggcatcg acgaggtcgc cgatcgggcg ctcgtgcatg cgcggcacgc
     2641 ccgagagctg gaacgcgccg ccctgcagcg tggcgagcac cgccagcgcc gccgtcagcg
     2701 gccgcatcgc ggtgccggcg ttgccgagga acagcgccgc ctcgcgcgcg cgcag
//
LOCUS       DASVLN010000535         8362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_349625, whole genome shotgun sequence.
ACCESSION   DASVLN010000535 DASVLN010000000
VERSION     DASVLN010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349625"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VF453_12940"
     CDS             <1..400
                     /locus_tag="VF453_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX7688610.1"
                     /translation="ASAASAAAGAASRPASGATPAARAAPLMQPEHWTITFADPKSMV
                     QAEYWVGNRSVSIRRTDNGVVAMLANLHKGVGMPVGWILLVDTLAGSLILLSLSGLAL
                     WMLTRRRRTLGLLVFGASLATVAGLALSHV"
     gene            508..2700
                     /locus_tag="VF453_12945"
     CDS             508..2700
                     /locus_tag="VF453_12945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7688611.1"
                     /translation="MSIRSRLLLLVFAVWLPALAGFGVLARSTYLHETEAATGHLRDA
                     ARSIGFVIDGELDKRAEIARVLGSSRALGEGDLQRFYEHARRATEGTTNTVILVDEAR
                     QVINTKKPYGTPLPARGWIPTPPLVTGAPVVTDLLTSRVDGAPVLTVMAPEPGGSPQR
                     YNIGIAFSPDVIQKTLEQQQLPAGAVVSVIDARFRTVARSRDPRKWLGVRAAQDTVDA
                     IGRGAFDRVFQSQTHEGEPTLAVVERSPRYGWYVAAGLPRSALDEPAIRVTLQAFSAA
                     GALAVIGLGLALYAARKIGQPVLALSDAAAELGRDRVPAPLRTGLSEADQVGAALHEA
                     GVRSARATEVLEHRVAEAIRQTQEAQTRLLDGQKHEAIGRLTGGLAHDLNNFLQTMST
                     GLQVLDRLVKDEGGRRVIEASTRALNKAATLVRQMLAFGRSQPLNPQAIDLHDFVLRC
                     EDLMGRAVGGRVRLVADIEPGLPPLRADPTQLELALLNLVFNARDAMPDGGTVTIRGR
                     LASATQTAALAPGRYLAVDVADTGGGMEPEVLQRVFEPYFTTKPVGVGSGLGLPQVRA
                     FAVQSGGAATIGSERGVGTTVSILLPVSDEPVDSRPAALAGHESFAPLRVLFVEDDLL
                     VSSVVVPALRAAGHSVKHCLTGEAALRALQDGQLHDVVFTDVVMPGSVSGLDLAAWCQ
                     ANMPGMAVIVATGYTTQHIDPGIKVLSKPYSMEDLLAELQVAARLAKS"
     gene            2788..3858
                     /locus_tag="VF453_12950"
     CDS             2788..3858
                     /locus_tag="VF453_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX7688612.1"
                     /translation="MTEVRKGQAPGHVDRAEFGRRWRDSFFDPAFDGERDAIARLEQI
                     AWEAYEAGRKAPVTRKAGAGFADPDYDLSVEWLAARDAIETARAENADPAQPSRVLLV
                     IGSPRNDGSCPGEVSKSFRLARWMQEEVEGQGIEVDVLDLSLLTSEYGRRIHPCKGCV
                     STAMPLCHWPCSCYPNHALAQTGDWMNELYPRWVRAHGVIVVSPVHWYMAPTVLKAMM
                     DRLVCADGGNPDPTSTQGKKAELAKAIEAGGWDFPQHLAGRVYGLMMHGDVAGIEGSR
                     RALSDWLDWMGLVDAGFVAQLDRYVGYYEPYESSHEALDRDLPVQREARNVALAVAHA
                     VRCLRSGQLIPVQPKTRRVRPK"
     gene            3959..4204
                     /locus_tag="VF453_12955"
     CDS             3959..4204
                     /locus_tag="VF453_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688613.1"
                     /translation="MDYLQIAPFAAEPSFELRFVPLFGAHPPLAFDCGPDGAVDIDGL
                     SEPARRDYLFAHMLVGRDYASPTVRRVLRARASGAAR"
     gene            complement(4144..4617)
                     /locus_tag="VF453_12960"
     CDS             complement(4144..4617)
                     /locus_tag="VF453_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX7688614.1"
                     /translation="MERSTRQNAAIRRAIAEAGRPLSPAEVLEAARGEVAALGIATVY
                     RNLKALLDEGTVQVVTLPGEVARYEIAHHDHHHHFRCDACQRVFDVHRCPGDLAALAP
                     PGFSVDRHEITLYGRCAECRRATPAGTRSPARGATLQRAAPDARARRTRRTVGEA"
     gene            4699..5448
                     /locus_tag="VF453_12965"
     CDS             4699..5448
                     /locus_tag="VF453_12965"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX7688615.1"
                     /translation="MVIALSPVAFTAAAVVSTLAGGAMALRFRDRLHYLLGFTAGVLL
                     GVVAFDLLPEIFELAHRHAFEPTPAMVALVVGFLLFHGLEKFVLVHPAQEEHYSHHHH
                     PQAGVASALVLAAHSFMDGVAIGIGFQVSPAVGLTVAVAVVAHDFSDGLNTVSLMLRH
                     HNTTRRAVAMLALDALAPMAGVASTLAFTIPPLQLMAYLGFFAGFLLYIGVSDILPEA
                     HSRAGPGVALRLIALTCCGAAFVYGVVRFGP"
     gene            5792..6517
                     /locus_tag="VF453_12970"
     CDS             5792..6517
                     /locus_tag="VF453_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688616.1"
                     /translation="MSLIQSSSTTLSSPFATMTATSSKAAHGERAEREGPVQSAAHDA
                     AGAIGAAASATVSFSDKALQALEDAGGYVADAVETGAREVGHAAEFVYDTAKQGLGEV
                     VVGAGDAVAEGYKVAKAGIEDAGEEAWKLAKAGVNGAIEVAGDGLKALANGVTEVENL
                     ASEGWNNVTAGVHRMTTAASTIGSDAYTMASKASDIALTVGSTVGSAAGTVLNGVEDV
                     AGTAASYATLGVAAGGKIVSAWI"
     gene            6577..7113
                     /locus_tag="VF453_12975"
     CDS             6577..7113
                     /locus_tag="VF453_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HEX7688617.1"
                     /translation="MRHALALIAAAVVVLPGCSVLSPTPVIELVKAGGAAAASAIAHG
                     PSSAKNTVYHEHKSLDAVCVEYNPQTPDPDIVPALQAELKVHEVDSRVFESGGTPDSC
                     HFVLSYTADIEWGTPPLASGYKSYIRDATLTLRDDQGKVLASSAYSLDGAFQMGKWSP
                     SRSKLAPVVTALVTGFDR"
     gene            7168..7875
                     /locus_tag="VF453_12980"
     CDS             7168..7875
                     /locus_tag="VF453_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX7688618.1"
                     /translation="MSAVQTFVRSSCIAAAVGAALAGCSSPSPLVQGPVAVRPVPQPS
                     YVEHAATGGIYRANMNNVSLFSDQRKPQAIGDTIKIDISENLNATNKMNSVNSRANSV
                     SSKGPGTSSDGLGSLLKGLANMNVTASGSDAFTGKGDSQNQTQFTGRISAFVINVQAN
                     GNLVVAGERNIAFNGTTNTLRFSGVVNPIDIKAGGIVASGDVADAKLELVGSGGNQDA
                     AQRGWLQKLLTDGLSVW"
     gene            8052..>8362
                     /locus_tag="VF453_12985"
     CDS             8052..>8362
                     /locus_tag="VF453_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7688619.1"
                     /translation="MITCLIVDDDPVLRELVGDHLTRYGLQARLADSAAAMREELERG
                     GIDIILLDLMLPDGDGIELCRDLRTTSKMPVIMLTAQGDPISRVLGLEMGADDYVAKP
                     F"
BASE COUNT         1158 a         3073 c         2936 g         1195 t
ORIGIN      
        1 ggcgtccgcg gcctcggcgg cggccggcgc cgcgtcgagg cctgcgtccg gcgcgacgcc
       61 ggcggcccgg gccgcgccgc tcatgcagcc cgagcactgg acgatcacgt tcgcagatcc
      121 gaagtccatg gtgcaggcgg agtactgggt cggcaaccgc tcggtgagca tccgccgcac
      181 cgacaacggg gtcgtcgcca tgctcgcgaa cctgcacaag ggtgtcggca tgccggtcgg
      241 ctggatcctg ttggtggata ccctggccgg tagcctgatc ctgctgtcgc tgtcggggct
      301 ggcgctgtgg atgctgacgc gccggcggcg cacgctgggc ctgctcgtgt tcggcgcctc
      361 gctcgcgacg gtcgcggggc ttgcgctgtc gcacgtctga cctggccccg gcgccgcggc
      421 gccactgtgc tcggccgcgg gattttgcgc ggcagcagcg gccttttcgg gtaacgtcgg
      481 cgcctcgccc aggacagaag gccccgcgtg agtatccggt cccgactgct gctgctcgtg
      541 ttcgccgtct ggctgccggc cctggccggt ttcggcgtgc tggcgcgctc gacctacctg
      601 cacgagacgg aggccgccac cggccacctg cgcgacgccg cgcgttccat cggcttcgtg
      661 atcgacggcg agctggacaa gcgtgccgag atcgcgcgcg tgctgggctc gtcgcgtgcg
      721 ctgggcgagg gcgacctgca gcgcttctac gagcacgcca ggcgcgcgac cgagggcacc
      781 acgaacaccg tgatcctcgt cgacgaggcg aggcaggtga tcaacaccaa gaagccctac
      841 ggcacgccgc tgccggcgcg cggctggatc ccgacgccgc cgctcgtgac gggcgcgccg
      901 gtcgtgaccg acctgctcac gagccgcgtc gacggggccc cggtgctgac ggtgatggcg
      961 ccggagccgg ggggctcgcc gcagcgctac aacatcggca tcgcgttctc gccggacgtg
     1021 atccagaaga cgctggagca gcagcagctg cccgcgggcg cggtcgtctc cgtcatcgac
     1081 gcgcgcttcc gtaccgtcgc ccgcagccgc gacccgcgca agtggctggg cgtgcgcgcg
     1141 gcgcaggaca cggtcgacgc gatcggccgc ggcgcgttcg accgcgtgtt ccagtcgcag
     1201 acgcacgagg gcgagccgac gctggccgtc gtcgagcgct cgccgcgcta cggctggtac
     1261 gtcgcggcgg ggctgccgcg atccgcgctc gacgagccgg ccatccgggt gacgctgcag
     1321 gccttctcgg cggccggcgc gctggcggtg atcgggctcg ggctggcgct gtacgccgcg
     1381 cgcaagatcg gccagccggt gctggcgctg agcgacgctg ccgccgagct gggccgtgac
     1441 cgcgtgccgg cgcccctgcg caccgggctt tccgaggccg accaggtcgg cgcggcgctg
     1501 cacgaggccg gcgtgcgctc ggcgcgcgcg acggaagtgc tcgagcaccg cgtggccgag
     1561 gcgatccggc agacgcagga ggcgcagacg cgcttgctcg acggccagaa gcacgaggcg
     1621 atcggccgcc tcaccggcgg cctcgcgcac gacctgaaca acttcctgca gacgatgagc
     1681 acgggcctgc aggtgctcga ccgcctggtc aaggacgagg gcggccggcg ggtgatcgag
     1741 gcgagcacgc gcgcgctgaa caaggcggcg accctggtgc ggcagatgct cgcgttcggg
     1801 cgctcgcagc cgctgaatcc ccaggcgatc gacctgcacg acttcgtcct gcgctgcgag
     1861 gacctgatgg ggcgcgcggt cggcggccgc gtgcgcctgg tcgccgacat cgagcccggc
     1921 ctgccgccgc tgcgcgccga cccgacgcag ctggagctgg cgctgttgaa cctcgtcttc
     1981 aacgcccgcg acgcgatgcc cgacggcggc acggtgacca tccgcggccg gctggccagc
     2041 gcgacccaga ccgccgcgct cgcgccgggg cgctacctcg ccgtcgacgt cgccgatacc
     2101 ggcggaggca tggagcccga ggtgctgcag cgcgtcttcg agccgtactt cacgaccaag
     2161 ccggtgggcg tgggcagcgg gctcggcctg ccgcaggtgc gcgcgttcgc agtgcagtcg
     2221 ggcggcgccg cgaccatcgg cagcgagcgc ggcgtcggca cgacggtgtc catcctgctg
     2281 ccggtgtcgg acgagccggt cgacagccgt cccgcggcgc tcgccggcca cgagagcttc
     2341 gcgccgctgc gggtgctgtt cgtcgaggac gacctgctgg tgtcctcggt ggtcgtgccg
     2401 gcgctgcggg cggccgggca ctcggtcaag cactgcctga ccggcgaggc cgcgctgcgc
     2461 gcgctgcagg acggccagct ccacgacgtc gtcttcacgg acgtggtgat gcccggctcc
     2521 gtcagcggcc tcgacctcgc cgcctggtgc caggccaaca tgcccggcat ggcggtgatc
     2581 gtggccaccg gctacacgac gcagcacatc gacccgggca tcaaggtgct ttccaagccg
     2641 tactcgatgg aggacctgct cgccgagctg caggtcgccg cccgcctcgc caagagttga
     2701 ggcggtccgg tccgctggga cgccacgcat cgcgccgggc gcctcgattg cctcggcccg
     2761 cggcacatca tcacggggac agcaggaatg accgaggttc gcaagggcca ggcgccgggg
     2821 cacgtcgacc gcgcggagtt cggccgccgc tggcgcgact cgttcttcga cccggccttc
     2881 gacggcgagc gcgacgcgat cgcgcgcctc gagcagatcg cctgggaggc ctacgaggcc
     2941 ggccgcaagg caccggtcac gcgcaaggcc ggcgcgggat tcgccgatcc cgactacgac
     3001 ctgtccgtcg aatggctcgc cgcgcgcgac gcgatcgaga ccgcgcgcgc ggagaacgcc
     3061 gaccctgcgc agccgtcgcg cgtgctcctc gtgatcggct ctccgcgcaa cgacggcagc
     3121 tgcccgggcg aggtctccaa gagcttccgc ctcgcgcgct ggatgcagga ggaagtcgaa
     3181 gggcagggca tcgaggtcga cgtgctcgac ctgagcctgc tgacctcgga atacggccgc
     3241 cgcatccatc cgtgcaaggg ctgcgtgtcg accgcgatgc cgctgtgcca ctggccctgc
     3301 agctgctacc cgaaccacgc gctcgcccag accggcgact ggatgaacga gctctacccg
     3361 cgctgggtgc gcgcgcacgg cgtgatcgtc gtctcgccgg tgcactggta catggcgccg
     3421 accgtgctca aggcgatgat ggatcgcctc gtgtgcgccg acggcggcaa tccggacccg
     3481 acctcgaccc agggcaagaa ggccgagctc gccaaggcga tcgaggccgg cggctgggac
     3541 ttcccgcagc acctcgccgg tcgcgtctac gggttgatga tgcatggcga cgtcgcgggc
     3601 atcgagggct cgcggcgcgc gctctcggac tggctcgact ggatgggcct cgtcgacgcg
     3661 ggcttcgtcg cccagctcga tcgctacgtc ggctactacg agccctacga gtcgagccac
     3721 gaggcgctcg accgcgacct ccccgtccag cgggaggcgc gcaacgtcgc gctcgccgtc
     3781 gcccacgcgg tgcggtgcct gcgcagcggg cagctgatcc cggtgcagcc caagacccgc
     3841 cgcgtgcggc cgaagtgagc ggagtggcca caggacgaca gcgccatggc gcgtcgtccg
     3901 acaggcgcgg gcttcccgat tgcatggtgc agtgcaagac aggcaccgga gcacgcacat
     3961 ggactacctg cagatcgccc ccttcgccgc cgagccgagc ttcgagctcc gcttcgtccc
     4021 gctgttcggc gcgcaccccc cgctcgcgtt cgactgcggc cccgacggcg cggtcgacat
     4081 cgacgggctg agcgagcccg cgcggcgcga ctacctgttc gcgcacatgc tggtcggccg
     4141 cgactacgcc tcgccgaccg tccgccgcgt cctgcgggcg cgcgcatccg gcgcggcgcg
     4201 ctgaagcgtc gccccgcgcg ccggcgaccg cgtgccggcg ggcgtcgcgc gccggcattc
     4261 ggcgcagcgg ccgtagagcg tgatctcgtg gcggtcgacg ctgaagcccg gcggggcgag
     4321 cgccgcgagg tcgccggggc agcgatgcac gtcgaacacg cgctggcagg cgtcgcagcg
     4381 gaagtggtgg tggtggtcgt ggtgcgcgat ctcgtagcgc gcgacctcgc ccggcagcgt
     4441 caccacctgc acggtgcctt cgtcgagcag cgccttcagg ttgcggtaga ccgtcgcgat
     4501 gcccagcgcc gcgacctcgc cgcgcgcggc ctccagcact tccgcgggcg acagcggccg
     4561 gccggcctcg gcgatcgccc ggcggatggc ggcgttctgg cgggtggagc gttccatggt
     4621 ggggcgggca gggcgacatg cccaccccgt cattgtggca cggtcgggat gatattgagg
     4681 atgcattatc attaacgaat ggtcatcgcc ctgtcgcccg tcgcgttcac ggccgccgcc
     4741 gtcgtctcga cgctggccgg cggggcgatg gcgctgcgct tccgcgaccg cctgcactac
     4801 ctgctcggct tcacggcggg cgtgctgctg ggcgtcgtcg cgttcgacct gctgccggag
     4861 atcttcgagc tggcgcaccg ccatgccttc gagccgaccc cggcgatggt cgcactggtc
     4921 gtcggcttcc tgctgttcca cgggctcgag aagttcgtgc tcgtgcatcc ggcgcaggaa
     4981 gagcactact cgcaccatca ccacccgcag gcgggcgtgg cgtcggcgct ggtgctggcg
     5041 gcgcacagct tcatggacgg cgtggcgatc ggcatcggct tccaggtctc gccggcggtg
     5101 gggctgacgg tggcggtcgc ggtcgtcgcg cacgacttct ccgacggcct gaacacggtc
     5161 agcctcatgc tgcgccacca caacacgacc cgccgcgccg tggcgatgct cgcgctggat
     5221 gcgctcgcgc cgatggccgg cgtggcctcg acgctcgcct tcaccatccc gccgctgcag
     5281 ctgatggcct acctcggctt cttcgccggc ttcctgctgt acatcggcgt ctccgacatc
     5341 ctgcccgagg cgcactcacg tgccggcccg ggcgtcgcgc tgcggctgat cgcgctgacc
     5401 tgctgcggcg cggccttcgt ctacggcgtc gtgaggttcg ggccctgagc ctggcgtcgc
     5461 gccgcgtgac gcgcgggcgc cacagccgag gtaaagatgt gtcgccgtgc ggcgtggatc
     5521 cgcgcgccgg cttgcgccag tctttcatgt cgggttcgat acaaacgccg gaaatcgtgt
     5581 cgattccgcc gtcgtttccg ggccgtgcac gaggcggcgg gcggctatcg tcctcgtggc
     5641 ccgagcccct tgccggtcgc gcttcggatt ccttcacgcg cgcgccgcaa cttctccggc
     5701 tcgatatagg ggttcatttc cgccgctaat tgccgattcc gcccgcgccc gactccctac
     5761 gattcgagca tccgaatcaa ggagtcgctc catgtctctc atccagtctt ccagcacgac
     5821 gctctcgtcg cccttcgcga cgatgaccgc cacgagcagc aaggctgcgc atggcgagcg
     5881 cgcggaacgc gaaggtcccg tccagtcggc ggcgcatgac gccgccggcg ccatcggtgc
     5941 cgcggcgagc gccacggtct cgttcagcga caaggcgctg caggcgctcg aggacgccgg
     6001 cggctacgtc gccgatgccg tcgagacggg cgccagggaa gtgggccacg ccgccgaatt
     6061 cgtctacgac accgccaagc agggtctggg cgaggtcgtc gtgggcgcgg gcgacgcggt
     6121 cgccgaaggc tacaaggtcg ccaaggccgg catcgaggat gcgggcgagg aagcctggaa
     6181 gctcgccaag gcgggcgtca acggcgcgat cgaggtcgcc ggcgacggcc tgaaggccct
     6241 ggccaatggc gtcaccgagg tcgagaacct cgcctccgag ggctggaaca acgtcaccgc
     6301 cggcgtccac cgcatgacca ccgccgccag cacgatcggc agcgacgcct acacgatggc
     6361 cagcaaggcc agcgacatcg cgctgaccgt cggctcgacg gtcggcagcg ccgccgggac
     6421 ggtgctcaac ggcgtcgagg acgtcgccgg caccgccgcc agctacgcga cgctgggcgt
     6481 cgccgcgggc ggcaagatcg tcagcgcctg gatctgagga tccgacgccc gcgccgtccc
     6541 tcgccgtctc cgtccctgcc tcgcatcgaa agtcccatgc gccacgccct cgccctgatc
     6601 gcggctgccg tcgtcgtcct gcccggttgc tccgtcctca gtcccacgcc ggtgatcgag
     6661 ctggtcaagg ccggcggcgc ggcggcggcc agcgccatcg cgcacggccc gagcagcgcg
     6721 aagaacacgg tctaccacga gcacaagtcc ctcgacgcgg tctgcgtcga gtacaacccg
     6781 cagacgcccg accccgacat cgtccccgcc ctgcaggcgg aactgaaggt ccacgaggtc
     6841 gacagccgcg tcttcgaatc cggcggcacg cccgactcct gccacttcgt gctcagctac
     6901 accgccgaca tcgagtgggg cacgccgccg ctcgcctcgg gctacaagtc ctacatccgc
     6961 gacgccacgc tgacgctgcg cgacgaccag ggcaaggtgc tcgcgagcag cgcctactcg
     7021 ctggacggcg cgttccagat gggcaagtgg tcgccgagcc gaagcaagct cgcccccgtc
     7081 gtcaccgcgc tggtcaccgg cttcgaccgc tgagccctcg cgccaccgcc ttcctccgcc
     7141 ttcgaaccaa tccactgggt gcctgccgtg tccgctgtcc aaaccttcgt ccgctcgtcc
     7201 tgcatcgccg ccgccgtcgg cgcggcgctg gcgggatgtt cctcgccctc gccgctggtg
     7261 cagggcccgg tggccgtgcg gccggtgccg cagccctcgt acgtggagca cgcggccacg
     7321 ggcggcatct accgcgccaa catgaacaac gtctcgctgt tctccgacca gcgcaagccc
     7381 caggccatcg gcgacacgat caagatcgac atctccgaga acctgaacgc gaccaacaag
     7441 atgaacagcg tcaacagccg cgcgaactcg gtgtcgtcca agggccccgg cacgagctcc
     7501 gacggcctgg gctcgctgct caagggcctg gccaacatga acgtcacggc ctcgggcagc
     7561 gacgcgttca ccggcaaggg cgactcgcag aaccagacgc agttcaccgg ccgcatctcg
     7621 gccttcgtca tcaacgtcca ggccaacggc aacctcgtgg tggccggcga gcgcaacatc
     7681 gccttcaacg gcacgaccaa cacgctgcgc ttctcgggcg tggtcaaccc gatcgacatc
     7741 aaggccggcg gcatcgtggc ctcgggcgac gtcgcggacg cgaagctgga gctcgtgggc
     7801 tcgggcggca accaggatgc ggcgcagcgc ggctggctgc agaagctgct caccgacggc
     7861 ctgtccgtct ggtgatgcga gcgggcctcg gcccgcatgt ttccggctcg tgaacggcgg
     7921 cgcggcccgg gccccgtcgc cgtcgtccaa ggcgagcgac aagcccgcga tcggcccgcc
     7981 cctgcgcaat gacagcgcaa gactgccggg ctatcctgcg gccgcaagaa cgacccttgc
     8041 ccgcccgccg catgatcacc tgcctcatcg tcgacgacga ccccgtcctg cgcgagctcg
     8101 tcggcgacca cctgacccgc tacggcctgc aggcccgcct cgccgactcc gcggccgcca
     8161 tgcgcgagga actggagcgg ggcggcatcg acatcatcct gctcgacctc atgctgcccg
     8221 acggggacgg catcgagctg tgccgcgacc tgcgcacgac ctcgaagatg cccgtcatca
     8281 tgctgaccgc gcaaggcgac ccgatcagcc gcgtgctggg gctcgagatg ggcgccgacg
     8341 actacgtcgc caagccgttc ga
//
LOCUS       DASVLN010000536         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_349668, whole genome shotgun sequence.
ACCESSION   DASVLN010000536 DASVLN010000000
VERSION     DASVLN010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349668"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF453_12990"
     CDS             complement(<1..499)
                     /locus_tag="VF453_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688620.1"
                     /translation="MTMRPEDVAALFDLPPPPPAGPASAESGTAAADATDAAEAALAA
                     EMAAELAATPGAMGVATDAPDSRTGRRVEVSWPARMRLPGGRVIGLRVRNVSEAGVGL
                     ASDEPIPADTVVDLEMTVPALDGRGDGTSVEVAIRTTYTVAQGTAILCGGTWMRRPPA
                     SLALVN"
     gene            complement(512..>1954)
                     /locus_tag="VF453_12995"
     CDS             complement(512..>1954)
                     /locus_tag="VF453_12995"
                     /inference="COORDINATES: protein motif:HMM:NF040115.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688621.1"
                     /translation="LDPAHVAALSTAAIGALTTDQAAALPTDDLAALTQAQVHALATA
                     SIAALAPEQVQALTSTELGAFGGAQFAALNSDDVAVLSGDQLAGLTTAVVASLSVAQL
                     HELSADQIAGLATAQVAALTTTQVAALTDAQFEAFTSTQFGALTTAQLHVLTPTQFAG
                     LSTAEIAGLTTTQVHALSTDEVAAMTAAQIGVLSTDDIAAMSPTQVAAFTSDQVAAMT
                     GDQISALFSVTPIVLDLAGTGIHTTSAGAQGVNFDLAGTGHAQSVGWTGAGTGLLAID
                     LNGNGRIDDGKELFGVGTVLPDGTHPANGYQAMAQYDSNHDGKLDAHDAAWSKLRVWV
                     DANHDGVSQPGELLTMAQAGVASIDLHAQAAGTMQNGNLIGLVSSYTTTSGATHEAAD
                     VWFGREASSTAAASTASTATAPNLSDLLAAPATTELPGGTADASAAHAAPAAPAADTA
                     THAATATHALLMDHHALGGDDPHGHPPLLI"
BASE COUNT          227 a          703 c          751 g          273 t
ORIGIN      
        1 cgttcaccag cgccaggctc gcgggtggcc gccgcatcca ggtgccgccg cacaggatcg
       61 ccgtgccctg cgccaccgtg taggtcgtcc ggatcgcgac ctcgacggac gtgccgtcgc
      121 cacggccgtc cagcgccggc acggtcatct cgaggtcgac cacggtgtcg gccgggatgg
      181 gctcgtcgct cgccagtccc acgccggctt cggagacgtt gcggacccgc agcccgatga
      241 cgcgcccgcc gggcaagcgc atgcgcgccg gccacgacac ctcgacgcgc cggcccgtgc
      301 gcgagtcggg cgcgtccgtg gcgacgccca tggcgccggg cgtggccgca agttcggccg
      361 ccatctcggc cgcgagcgcg gcctccgccg catccgtggc gtccgcggcc gcggtgcccg
      421 actcggcaga agccggcccg gccggcggcg gcggtggcag gtcgaacagg gcggcgacat
      481 cctcgggacg catcgtcatg gcggtgcaag ctcagatcag cagcggaggg tggccgtgcg
      541 gatcgtcgcc gccgagggcg tgatgatcca tcagcagcgc gtgggtcgcg gtcgcggcgt
      601 gcgtcgcggt gtcggcggcg ggggcggcgg gggcggcgtg cgcggccgag gcgtccgccg
      661 tgccgccggg cagctcggtc gtggccgggg cggccagcag atccgaaagg ttgggcgcgg
      721 tcgccgtgct cgccgtcgaa gccgccgcgg tcgacgacgc ctccctgccg aaccagacgt
      781 cggcggcctc gtgcgtcgcg ccgctggtcg tcgtgtagct cgagaccagc ccgatcaggt
      841 tgccgttctg catcgtgccg gcggcctggg cgtgcaggtc gatcgacgcg acgcccgcct
      901 gcgccatcgt cagcagctcg ccgggctggc tcacgccgtc gtggttggcg tcgacccaga
      961 cccgcagctt gctccatgcc gcatcgtgag cgtcgagctt gccgtcgtgg ttgctgtcgt
     1021 actgcgccat cgcctggtag ccgttggccg ggtgcgtccc gtcggggagc acggtgccga
     1081 cgccgaacag ctccttgcca tcgtcgatcc ggccgttgcc gttcaggtcg atcgcgagca
     1141 gcccggtgcc ggcgccggtc cagccgacgg actgcgcatg gccagtgccc gcgaggtcga
     1201 agttcacgcc ctgcgcgccc gccgacgtcg tgtggatgcc cgtgccggcc aggtcgagca
     1261 cgatcggcgt cacgctgaac agcgccgaga tctggtcgcc cgtcatcgcg gcgacctggt
     1321 cgctggtgaa cgcggcgacc tgcgtcggcg acatcgcggc gatgtcgtcg gtcgacagca
     1381 cgccgatctg cgcggccgtc atcgcggcga cctcgtccgt cgacagcgca tgcacctgcg
     1441 tggtcgtgag gcccgcgatc tccgcggtcg acagcccggc gaactgcgtc ggcgtcagca
     1501 cgtgcagctg cgccgtcgtc agcgcgccga actgcgtcga ggtgaaggcc tcgaactgcg
     1561 cgtcggtcag cgcggcgacc tgggtcgtgg tcagcgccgc gacttgcgcc gtcgcgaggc
     1621 cggcgatctg gtcggccgag agctcgtgca gctgcgcgac cgacaggctc gccacgaccg
     1681 cggtcgtgag gccggcgagc tggtcgccgg agagcaccgc gacgtcgtcg gagttcagcg
     1741 cggcgaactg ggcgccgccg aacgcgccga gctcggtcga ggtcagcgcc tgcacctgct
     1801 cgggcgcgag ggccgcgatc gaggcggtcg cgagcgcgtg cacctgcgcc tgggtcagcg
     1861 cggcgagatc gtccgtcggc agcgccgcgg cctggtcggt cgtcagcgcg ccgatggccg
     1921 cggtcgacag cgcggccacg tgggcggggt cgag
//
LOCUS       DASVLN010000537         2415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_242163, whole genome shotgun sequence.
ACCESSION   DASVLN010000537 DASVLN010000000
VERSION     DASVLN010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242163"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VF453_13000"
     CDS             complement(<1..264)
                     /locus_tag="VF453_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688622.1"
                     /translation="MSTLESDADPDDFPAVPDPDATGPEDALADGWRRWRQALHRLPE
                     TGFDERRTAARIARTLELLGLEVHRGIGGTGVVGSLRVGGGTGA"
     gene            427..906
                     /locus_tag="VF453_13005"
     CDS             427..906
                     /locus_tag="VF453_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7688623.1"
                     /translation="MALDALDTRLLDLLQQDASLPIRALADRAGSSPATCQRRIARLR
                     ATGVLVRQVAIVDRHLVGRPLTAFVSVELDRQNAALLRAFEQRMAEQPEVMACYEVSG
                     ESDFLVIVIAESMDRYHAFTRTVFSSLNNVRNFKSMFAMNCSKFETRVPLPVPADAG"
     gene            1083..1388
                     /locus_tag="VF453_13010"
     CDS             1083..1388
                     /locus_tag="VF453_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688624.1"
                     /translation="MSSLHPTRAELLSDSAVYRRTRRGQRELLRADDLSASPALRILA
                     RINGFTDLRRLIDLAPGDVRELAASLEQLLDAGLIELVDGTDAPAEAEREASLRRPH"
     gene            complement(1450..2154)
                     /locus_tag="VF453_13015"
     CDS             complement(1450..2154)
                     /locus_tag="VF453_13015"
                     /inference="COORDINATES: protein motif:HMM:NF038131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor K domain-containing protein"
                     /protein_id="HEX7688625.1"
                     /translation="MRLNAILAGCALAFAALHAAADPVSGSTQGTWVDPNPSASPIVV
                     TGVGTSQFTWGRAAGTPTPNILTFTAAAGGFSSVTETPFKVGSISYYNGTTASGTTPD
                     SIQLALTLDFTDPAIPAVTSDYTFKLVSTPNGGQDPDADADYVYLPSAFSATSFVIGS
                     TTYNVKLTGFEDIVGDGFLTSDDLSFHVRENGTASADLFAVVTTQTAVPEPQSVALML
                     AGLGMMGLLARRRGRG"
BASE COUNT          346 a          856 c          868 g          345 t
ORIGIN      
        1 cgcgccggtg ccaccgccca cgcgcaggct cccgaccacg cccgtgccgc cgatgccgcg
       61 gtgcacctcc agccccagca gctccagcgt gcgggcgatg cgcgcggccg tgcggcgctc
      121 gtcgaagccg gtctcgggca agcggtgcag cgcctggcgc cagcgccgcc agccgtcggc
      181 gagcgcgtcc tccgggccgg tcgcgtccgg gtcggggacg gcggggaagt cgtcgggatc
      241 ggcgtcggat tccagggtgc tcatgcgggc tcctgcatcg gcatggccgc attgttcgcc
      301 ggggacgtcg agcggattca ccgctttcgc ggcgccagat gacgcatttg tctcacattc
      361 gatgcctaga atgagtcgat gccccgtcgc cccgcgcgcc ctgcggcacc gccgcctccg
      421 gtcagggtgg cgctggacgc cctcgacacc cgcctgctcg acctgctgca gcaggacgcg
      481 tcgctgccga tccgcgcgct cgcggacagg gccggcagct cgcccgcgac ctgccagcgc
      541 cgcatcgcgc gcctgcgcgc gacgggcgtg ctcgtgcgcc aggtcgccat cgtcgaccgc
      601 catctcgtcg ggcggccgct cacggcgttc gtctcggtgg agctcgatcg ccagaatgcc
      661 gcgctgctgc gcgccttcga gcagcgcatg gccgagcagc ccgaggtgat ggcatgctac
      721 gaggtctccg gcgagtccga cttcctcgtc atcgtgatcg cggagtcgat ggatcgctac
      781 cacgcgttca cgcgcaccgt cttctcgagc ctgaacaacg tgcgcaactt caagagcatg
      841 ttcgcgatga actgctccaa gttcgagacc cgggtgccct tgcccgtacc ggcggatgcg
      901 ggctgaacct cgacgagcgg gccaccggtc gccagcgccg ctcgagcctg ccgtcaacgg
      961 ccacccctgc cgacgatgca ggcaaacgcg acgacgaccg ggcccgcatg cgagacttgc
     1021 cgcaagagaa atgacaagaa gatgacgagg cccgcgcgat gccgtgggtt tccgatccac
     1081 cgatgagcag tcttcatccg acccgcgccg agttgctgag cgactcggcc gtctaccggc
     1141 gcactcgccg cggccagcgc gagctcctgc gcgccgacga cctctccgct tcgccggccc
     1201 tgcgcatcct cgcgcgcatc aacggcttca ccgacctgcg ccgcctgatc gacctcgcgc
     1261 ccggcgacgt gcgcgagctg gcggcctcgc tcgagcagct gctcgacgcc ggcctcatcg
     1321 agctggtgga cgggacggac gcgccggccg aggccgagcg cgaggcgtcg ctgcgccggc
     1381 cgcactgagg cggcgttgca atgcaaacgg cccgcatcgc gcgggccgtc atcgaagggc
     1441 ggaggacgct cagccgcgac cgcggcggcg tgccagcagg cccatcatgc cgaggccggc
     1501 cagcatcagc gcgacgctct gcggctcggg cacggcggtc tgcgtggtga cgactgcgaa
     1561 caggtcggcc gaggcggtgc cgttctcgcg cacgtggaag gacaggtcgt ccgaggtcag
     1621 gaagccgtcg ccgacgatgt cctcgaagcc ggtgagcttg acgttgtagg tcgtgctgcc
     1681 gatcacgaag ctggtcgcgc tgaaggcact cggcaggtag acgtagtcgg catcggcgtc
     1741 cggatcctgg ccgccgttgg gcgtgctgac gagcttgaag gtgtagtcgc tcgtgacggc
     1801 cggaatcgcc gggtcggtga agtccagcgt cagcgcgagc tggatcgagt caggcgtcgt
     1861 gccggaggcc gtcgtgccgt tgtagtacga gatcgagccg accttgaacg gcgtctccgt
     1921 gaccgacgag aagccgcccg ccgccgcggt gaaggtcagg atgttgggcg tcggggtgcc
     1981 ggcggcgcgg ccccatgtga actgggacgt gccgacgccc gtgacgacga tcggcgaggc
     2041 cgacggattc ggatcgaccc aggtgccctg ggtggagccc gagaccgggt cggcggccgc
     2101 atgcagcgcg gcgaaggcga gcgcgcagcc cgccaggatg gcattgagac gcatgagggg
     2161 aaacctccga gggatttgtg atggtgcgcg ccgccccgtc cgtggccggt cgtgcgatcg
     2221 cgtgcgccgc actgtcgtcg tttccgcagc gcagcaacat cccccatcag gaggatcccc
     2281 ctaactcgtg ggagccgcgc gcggaggcct caccaccagg cctcgcagtc gacgcccgcg
     2341 ctgtccggcg cgccgccgca ggcgaggacg gagccgtccg gcagcagggt cgcgccggcg
     2401 tgggcgcgca ggacc
//
LOCUS       DASVLN010000538         3320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_513375, whole genome shotgun sequence.
ACCESSION   DASVLN010000538 DASVLN010000000
VERSION     DASVLN010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513375"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..885
                     /locus_tag="VF453_13020"
     CDS             322..885
                     /locus_tag="VF453_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7688626.1"
                     /translation="MAALAFSCCAQAQSGSLSSSTGSTRPGKGFLDINVGRARYDTPC
                     GVPGLTCQNNVTSYSVTAGNMFTENFGAELTYLNFGTAHRAGGGVTARGLNLSVVGRV
                     PLGEYFGLSGKVGTTYGITHVNAPDSAGVPTGRAKGFGLGYGVGLDVKVAHNLRGEVG
                     WERHDFHFAGSGTSNVRNVTLGLNYTF"
     gene            952..1254
                     /locus_tag="VF453_13025"
     CDS             952..1254
                     /locus_tag="VF453_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688627.1"
                     /translation="MNAHPLKHDVPGHRPGTTHGETIAAGDVGVDDRSGRAPLPHEQD
                     ESSHSQASAAEGHRRVGGQAYADTMEGRQDTDRGPVMDEVYHRMASTGRGGEAPRR"
     gene            1584..3035
                     /locus_tag="VF453_13030"
     CDS             1584..3035
                     /locus_tag="VF453_13030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX7688628.1"
                     /translation="MGSMDFRTEQRQQQTLSPRLQHAVRLLQLSSLEFAQQVHDALGK
                     NPFLEEDTDGEETPEQTAAAEEAQAAQEAIAAANETMAPPEGELPGEGDRDMWQADGL
                     GSGKRADDDETSALDMMAVPTSLATHLHGQLAVLPLSLRDLVLAKVIVESLDDDGYLR
                     TSLDEMIEIADLDPPATQEEMQIALRRVQALEPAGVAARSVSECLLAQLGSIEDADAR
                     DIAHRIISDHLNCLAAKDVTTMARALGRTPAEIEAVCDRIRRLDPRPGWRFGSTQLQY
                     VIPDVIVRKVRGQWNVQLNPAIIPKVRLNQVYAELFQRHRRAQNAEMATHLQEAKWTM
                     RNVEQRFSTILSVAEAIIRRQKHFLEFGAMAMKPLGLREIADELGLHESTVSRVTNNK
                     YMATPIGVFELKYFFSRAMTTASGGACSGTAIRGLIKDIIEAESPTAPLSDAEIARQL
                     ATQGLQVARRTVTKYRQLLKIEAVERRRRHAPA"
BASE COUNT          521 a         1209 c         1159 g          431 t
ORIGIN      
        1 gcggcgggcg tgcgagcgcc ggcgcgcgga acgggagggg cgcggacatg ggcgggtggg
       61 ctcctgcggc ctcgccatgc gcgccctcgt ggcgcgacgc gagccggacc cggcttcggc
      121 aacgcccatg cccgcgcttc gccgccgccg acatggcgcg ggtggcgtcc cacgcgcagg
      181 taggaatgca ccttcgtggc acgggcgttg ccggttcggg ccaggcatcc ttccgccatc
      241 cacccttcac cacaaccgcg attcgacaag gggaccctca tgaacagcgc gtgtcgtgca
      301 ctctccggtg gactggtcgc cgtggcagcg ctcgcattct cgtgctgcgc gcaggcccag
      361 tccggttcgc tgagctcctc caccggctcg acccggcccg gcaagggctt tctcgacatc
      421 aacgtcgggc gcgcccgcta cgacacgccg tgcggcgtgc cggggctcac gtgccagaac
      481 aacgtgacct cgtacagcgt gaccgccggc aacatgttca ccgagaactt cggcgccgag
      541 ctgacctacc tgaacttcgg caccgcgcac cgcgcgggtg gcggcgtgac cgcgcgcggc
      601 ctcaacctga gcgtggtggg ccgcgtgccg ttgggcgagt acttcggcct gtcgggcaag
      661 gtgggcacga cctacggcat cacgcacgtg aacgcgcccg acagcgccgg cgtgccgacc
      721 ggccgggcca agggcttcgg cctcggctac ggagtcgggc tcgacgtgaa ggtcgcgcac
      781 aacctgcgcg gcgaggtcgg ttgggagcgg cacgatttcc acttcgccgg ctcgggcacg
      841 tccaacgtcc gcaacgtgac gctcggcctg aactacacct tctgagccgg cgcgccgcga
      901 gggcgcgcgg catgggcggg cgaccctcgc gggcgcacgc gggagacgac gatgaacgcg
      961 catcccttga agcacgatgt ccccggccac cggcccggga cgacgcacgg cgagaccatc
     1021 gcggccggcg acgtcggcgt ggacgaccgc agcgggcgcg caccgctgcc ccacgagcag
     1081 gacgagtcca gccacagcca ggcctcggca gccgagggcc accgccgggt cggcggccag
     1141 gcctacgcgg atacgatgga gggccgccag gacaccgacc gcggcccggt gatggacgag
     1201 gtgtaccacc gcatggcctc gacgggtcga ggtggcgagg cgccgcgtcg ctagcggccg
     1261 cgaccgcctc ttccagcacg gccgggcacg caggcgacag gcccttgcgc ccgctgccgg
     1321 ccttgcggca aatctggggc ccggccgcgt tgccggacgt ggaaaaacgc ggccgaagcg
     1381 gccaaaacgc gtgtaacgaa ttcctttctt ccgcgtttcg ttgcgcaccg gcgacagaac
     1441 tgtctcgtca tcgcaacgaa cgcgccccat tcgagccgtc gtgacggaat tggcacgggc
     1501 gttgcaatcc atgagccatc gacgcggcgt cggcaagtct tgccggcggc cgcgagcttc
     1561 aacgacctgc tcaggagacc cccatgggtt cgatggattt ccgcacagaa cagcgccagc
     1621 agcagacgct gtctccccgc ctgcagcacg ccgtccgact gctgcagctc tcttcgctcg
     1681 agttcgcgca gcaggtccac gacgcgctcg gcaagaatcc gttcctcgag gaagacaccg
     1741 acggcgagga gacgcccgag cagacggccg ccgccgagga ggcgcaggcc gcccaggagg
     1801 cgatcgccgc cgccaacgag acgatggcgc cgcccgaggg cgagctgccc ggcgagggcg
     1861 accgcgacat gtggcaggcc gacggcctcg gctcgggcaa gcgcgccgac gacgacgaga
     1921 ccagcgcact cgacatgatg gcggtgccga cctcgctcgc gacgcacctg cacggccagc
     1981 tcgcggtgct gccgctgtcg ctgcgcgacc tcgtgctcgc caaggtgatc gtcgagtcgc
     2041 tcgacgatga cggctacctg cgcacctcgc tggacgagat gatcgagatc gccgacctcg
     2101 acccgccggc gacccaggag gagatgcaga tcgcgctgcg gcgggtgcag gcgctcgagc
     2161 ccgcgggcgt cgcggcgcgc agcgtctcgg aatgcctgct cgcgcagctg ggctcgatcg
     2221 aggacgccga cgcgcgcgac atcgcgcacc ggatcatctc ggaccacctc aactgcctgg
     2281 ccgccaagga cgtgaccacg atggcgcgcg cgctcggccg cacgccggcc gagatcgagg
     2341 ccgtgtgcga ccgcatccgg cgcctcgacc cgcgtccggg ctggcgcttc ggctcgacgc
     2401 agctgcagta cgtgatcccc gacgtgatcg tgcgcaaggt gcgcggccag tggaacgtcc
     2461 agctgaaccc ggccatcatc cccaaggtgc ggctcaacca ggtctacgcc gagctgttcc
     2521 agcgccatcg ccgcgcgcag aacgccgaga tggccacgca cctgcaggag gccaagtgga
     2581 cgatgcgcaa cgtcgagcag cgcttctcga ccatcctgag cgtggccgag gcgatcatcc
     2641 ggcgccagaa gcacttcctc gaattcggcg cgatggcgat gaagccgctc ggcctgcgcg
     2701 agatcgccga cgagctgggc ctgcacgagt ccaccgtctc gcgcgtgacg aacaacaagt
     2761 acatggccac gccgatcggc gtgttcgagc tcaagtactt cttctcgcgc gcgatgacga
     2821 ccgccagcgg gggcgcctgc tcgggcaccg ccatccgcgg gctcatcaag gacatcatcg
     2881 aggccgagtc gccgaccgcg ccgctgtcgg acgccgagat cgcgcgccag ctcgccacgc
     2941 aaggcctgca ggtggcgcgg cgcacggtga ccaagtaccg ccagctgctc aagatcgagg
     3001 ccgtggagcg gcgtcgccgt cacgcgccgg cgtgaggcgc cgcctcccat ggccctcggc
     3061 ccccgcacgc acgtcgatcg tggggtgcgg gcccggaatg ccgtttgccg ttccccggga
     3121 cctgccgccg cacgcacggc gcaggcccgc cgattcctct aggagtggag agtttcgtga
     3181 acgccgtgct gtcggatgaa gtcgcgcagc tgcgcgccga gctgcgccgg gcgcaggacg
     3241 ccctcgccga ggcctgcgcc gagcgcgacc tcgcgcgcga gcaggccgtc gaggcgcgcg
     3301 cgcagcgcta cgcgttcctc
//
LOCUS       DASVLN010000539         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_458900, whole genome shotgun sequence.
ACCESSION   DASVLN010000539 DASVLN010000000
VERSION     DASVLN010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458900"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1929)
                     /locus_tag="VF453_13035"
     CDS             complement(<1..>1929)
                     /locus_tag="VF453_13035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7688629.1"
                     /translation="APRVRVDDGSRIPRILRLTREQAWFCLSGIAASVLGWAITRGWN
                     VPVHVVGFISLPLLVWAAIKFPPLFVHTAVMATGLVTITIQLTAPGSASADPATHTAS
                     LFLFLLSVSMLTLIVNVVVQGQRRLAGELVYRTQQQRVELMLQAATDAVISFDEHGAL
                     SYWNPAAERLFGRSAAQAMGRPVHEILPIPKLGVAMATTGSIAALCDAGDELFSGVVI
                     AFDARDAIGRSLPVELSLTAYRTDRGWAATAFMRDARTHKRQEEQLRQARDKAEEATR
                     AKSLFLATMSHELRTPLSGIIGMLQLALREQMPPPARSRASLALGNAESLLAIINDIL
                     DFSKIEAGKMGFESVDFDIRDMVHSLIELLDLRAQEAGLSLLGDIDANVPRWLRGDPV
                     RLRQIMLNLIGNALKFTEVGTVQLRVACGSTETGRLLLEVEVEDTGIGISEEAQQRLF
                     RSFEQADLTTTRKYGGTGLGLTITRALVSGMQGEITLRSRPGEGSTFRVVLPVALGAP
                     VETVAEDEIAPFDCRLKVLCAEDGHTNRVIVQAFVQGLGHDVTFVENGLQAVQRCAEE
                     SFDLILMDGRMPVMDGLAAARAIRAGGIEDTWVLDPEIWMIALTANATGNDRDLCLAA
                     GMDDFLSKPVDERLLAQAL"
BASE COUNT          279 a          677 c          696 g          277 t
ORIGIN      
        1 agcgcctgcg cgaggaggcg ctcgtcgacg ggcttggaca ggaagtcgtc catgccggcg
       61 gccaggcaca ggtcgcggtc gttgccggtg gcgttggcgg tcagggcgat catccagatc
      121 tcgggatcga gcacccaggt gtcctcgatg cccccggcgc ggatcgcgcg cgccgcggcc
      181 aggccgtcca tgaccggcat gcggccgtcc atgaggatga ggtcgaagct ctcctcggcg
      241 cagcgctgca ccgcctgcag gccgttctcc acgaaggtca cgtcgtggcc cagcccttgc
      301 acgaaggcct gcacgatgac gcggttggtg tggccgtcct cggcgcagag caccttcagg
      361 cggcagtcga agggggcgat ctcgtcctcg gcgacggtct ccaccggcgc gcccagcgcc
      421 accggcagca cgacgcggaa ggtgctgccc tcgcccggcc ggctgcgcag ggtgatctcg
      481 ccctgcatgc cgctgaccag cgcgcgcgtg atcgtcaggc cgagtccggt gccgccgtac
      541 ttgcgcgtgg tcgtgaggtc ggcctgctcg aagctgcgga acaggcgctg ctgggcctct
      601 tccgagatgc cgatgccggt atcctcgacc tcgacctcga gcagcagccg cccggtctcc
      661 gtcgagccgc acgcgacgcg cagctgcacg gtgccgacct cggtgaactt cagcgcgttg
      721 ccgatcaggt tgagcatgat ctgccgcagc cgcaccgggt cgccgcgcaa ccagcgcggg
      781 acgttggcgt cgatgtcgcc cagcagcgac aggccggcct cctgcgcgcg caggtcgagc
      841 agctcgatca gcgagtgcac catgtcgcgg atgtcgaagt ccacggactc gaagcccatc
      901 ttgcccgcct cgatcttcga gaagtcgagg atgtcgttga tgatggccag cagcgactcg
      961 gcgttgccga gcgcgagcga cgcccgcgag cgcgccggcg gcggcatttg ctcgcgcagc
     1021 gcgagctgca gcatgccgat gatcccggac agcggcgtgc gcagctcgtg gctcatggtc
     1081 gcgaggaaca gcgacttcgc gcgcgtggcc tcctcggcct tgtcgcgcgc ctggcgcagt
     1141 tgctcctcct ggcgcttgtg ggtgcgcgcg tcgcgcatga aggcggtcgc ggcccagccg
     1201 cggtcggtgc ggtaggcggt cagcgagagc tcgaccggca gcgagcggcc gatcgcgtcg
     1261 cgcgcatcga aggcgatcac gacgccggag aacagctcgt cgcccgcgtc gcacagcgcg
     1321 gcgatgctgc cggtggtggc catcgccacc cccagcttcg ggatgggcag gatctcgtgc
     1381 accggccgcc ccatcgcctg cgcggcgctg cgtccgaaca ggcgctccgc ggccgggttc
     1441 cagtacgaca gcgcgccgtg ctcgtcgaag ctgatcacgg cgtcggtcgc ggcctgcagc
     1501 atcagctcga cgcgctgctg ctgcgtccgg tagaccagct cgcccgcgag ccggcgctgg
     1561 ccctgcacga cgacgttgac gatcagcgtc agcatcgaca cgctcagcag gaagaggaac
     1621 agcgacgccg tgtgcgtggc cggatcggcg ctggcgctgc cgggcgcggt gagctggatc
     1681 gtgatcgtca cgagccccgt ggccatcacc gcggtgtgaa cgaacagcgg cgggaacttg
     1741 atcgcggccc agaccagcag cggcagcgag atgaagccga ccacgtgcac cggcacgttc
     1801 cagccgcgcg tgatcgccca gccgagcacg ctcgcggcga tgccgctcag gcagaaccag
     1861 gcctgctcgc gcgtgaggcg caggatgcgc gggatgcgcg agccgtcgtc cacgcgcacg
     1921 cgcggcgcg
//
LOCUS       DASVLN010000540        11022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_242824, whole genome shotgun sequence.
ACCESSION   DASVLN010000540 DASVLN010000000
VERSION     DASVLN010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242824"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..840)
                     /locus_tag="VF453_13040"
     CDS             complement(31..840)
                     /locus_tag="VF453_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX7688630.1"
                     /translation="MTDQRPVNLDFDLPAQPQSLVKLAALLREDDVNLQQIGALIDSD
                     MSFAAAVMKAVNSPLYGLKGRVQSVQQAVTYLGVREISAIAYETGLQAAFPQVPQLVA
                     VWERARLRGLLMGRLAQALSMEAWGAHTAGLFEECGKAVLFRHDAARYAPMLAQAAND
                     EALLELERQAFATTHEEVGARLCEAWGLAPAAVASVRHHVEIQSTMRLPRVSHRYICV
                     LSALAHTLVTEPGKLEDVAMKLAPQAMLDQGSLLRGLHRIKDKIDEALASA"
     gene            1052..2314
                     /locus_tag="VF453_13045"
     CDS             1052..2314
                     /locus_tag="VF453_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic transporter"
                     /protein_id="HEX7688631.1"
                     /translation="MTDWPAPTVPNLAIWTIAAIATAGVILRPGRVPQAAWALAGAAA
                     LVVTGLLPLPSALRGLAESVDVVLFLAGMMLLSELARREGLFDTLAGWAVRHADGSAP
                     RLFTLIYGVGVLVTVFLSNDATAVVLTPAVCVAARRAKAAHPLPYLFACAFVANAASF
                     VLPIANPANLVVFGTHLPPLDAWCRRFALPSIAAIVATWALLRWAMRARLREPLERVD
                     DVPPLDFTGLLAAVGVAGVVLAMLVASALGAALGLPTLAATLLAVGGVQWAKRESPLP
                     LLEHVAWNVLVMVAGLFVIVEGLVRTGLVGALAEALRDLAGHSPHAAAWAAGMVTGLG
                     GNLLNNLPAGLLAGMAVNAGPVDDAVRSAVAIGIDLGPNLSITGSLATILWLAAIRRE
                     GEHVSGVEFLKMGAIVMPLALVVTLALV"
     gene            2671..4680
                     /locus_tag="VF453_13050"
     CDS             2671..4680
                     /locus_tag="VF453_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688632.1"
                     /translation="MNHKPRGLRRTVSLASAALGLGAAAAQAATSYPFNDGVVVALQP
                     ASGTTITVATPTAGAAFQGIGTNLGQLGDMGYIPCAQGSTGCTAAPGSPKISAKSVRA
                     SINLPFNAQGSWTLVNQVCTPVTVTGLGVKFGDPKGTLGFFDTYGATTFYSDAQCSSG
                     ASMQDRFGSAANCTDTSPANYRGACLMDGVRMRNLDWLEVYFAAPAEYMQSCGSGCDK
                     LLGDTATMQNFAMYKAAQLVYQGRFGSAPAAPKYVELFNEPEGTWNVQVTPAQYDQFV
                     GYFMTAMQSLVAQRLKTAGLSQAEKTYLGDLQHVTVMAGPALGSGYLLDYVGDSAPWD
                     TSKDWFASAAEASSFYATLAANNAVVSTHLYDDKRTGAQFTGLNVMTGLGQLLAVRNA
                     SSSNAAVVISEAAETVQNDDVAVNGYRGFCDANTPRNDKKSTEGACDAMRPGDPASVG
                     YSYPDCIPGATYVSSTAGKYAGPYQGTALADFCHHQAAKLYRNLANYANQNDQHATSV
                     NGVVSELIWASSPTPSSSEHGLVTRQGLPSLMNVGTDPLLATFSADGYATVFPVTFAQ
                     DFDLGAGIFASAATNPQRARLALANTASVTKTVTLNFGSTPLRQFTRVLDGSSFGGTR
                     DKYTGQLESMASGLQAPAGNNFGSRNYNLVVTLPANSAVTLTLAP"
     gene            complement(4743..6254)
                     /locus_tag="VF453_13055"
     CDS             complement(4743..6254)
                     /locus_tag="VF453_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX7688633.1"
                     /translation="MTTPTPAATSWAERAGALRIDGRMLVDGERRATQSGETFAKHSP
                     IDARLLGPIARGVAADVDLAVRAARAAFEDGRWAGKAPAQRKKILQRFSDKILAAKDE
                     LALLETLDMGKPIQYSLAVDVPACARCIAWYAEAVDKVYDEIAPTAANALALITREPM
                     GVIGVIVPWNYPMIMAAWKLGPALAAGNSVVLKPSEKSPLTALRLAELALEAGVPPGV
                     FNVVPGYGHEAGEALALHMEVDAIGFTGSTRVGRRMYEYAGKSNLKRVYNELGGKSAF
                     VVFDDFKDLERAARTVAGSMFFNQGESCNAPSRVLVHERVADEFVKIVAAEAPKYAPA
                     DPLDAKTEMGALVDDTQLRTVLGYIDAGHAEGARVVAGGRQARAETGGYYVEPTVFDG
                     VANSMKIAQEEIFGPVMSVIRFKDEREAVALANGSSYGLQGSVWSDDINRAHRVARAL
                     RAGTIHVNSYDEDDITVPFGGYKQSGNGRDKSLHAFDKYTELKTTWIRIDGAK"
     gene            complement(6449..9262)
                     /locus_tag="VF453_13060"
     CDS             complement(6449..9262)
                     /locus_tag="VF453_13060"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7688634.1"
                     /translation="MSRKKNAPPAWRPAAIVAAMQVACLQAHAQDAAPAPAVQQVTVT
                     GSNVRRTDSETPSPVQVLSAEDIRNSGYTSVGDVLHSITANNMGSLSQSAPSAFAAGG
                     SGVALRGLTVGATLVLIDGHRMASYPMPDDGERDFVDISSIPMEAIERIEVLKDGASA
                     VYGSDAIAGVVNVILKKSFTGTTVSAEGGVTSHGDGATTHGSFITGWGDLAKDGHNAY
                     VALEARHQQDILLRKRAYLARTDWRPYGGVDLTQGTGSGAVSGASSGAGSGTGYLTDS
                     SGNPVYYFPGCTDAQAAADTCGYSNPYQTLQPSTTNIDLLGRYTTKLGGGWEANLQGS
                     ALRSEAHQVGLFNNAAATGVGSGGNGGGVNLFHFGPGSAPAPAFPDQFPFVLTVPANY
                     PGNPTGTDAPLVYDFLDIGPLEQTVVTTSYRTVAEAQGSVGEWDLNFSGGVTRAVTRA
                     SFDHYIDFPAMQQALDDGTYQVGGDNSAILSTVAPHASSVSTNELSFLTARGTRTLTD
                     LPGGPLALGLGVDLTHRRLHEIFPPSFVNGAQAIGIYSFAQGSQTVAATYAELVAPVS
                     KQLEIDLAGRVDHYNTYGTSATPKAGFKWSPAREFTLRGTYSKGFRAPNPAETGDAGS
                     TSGFVGNLHDPVYCPGGTPAANLPAGMLDPCNIALQELQLSSPHLKPEKSQSFTLGLI
                     LEPSKSYSATLDYYHIQIRNQIISVGQLGQIGLDDAAALGTVIYRDSSQNILYDTYPF
                     INANTTTTSGLDLDVLGKYGLGAAGSLKAELQVSYTLAYDLTAQGVKYHLAGTHGPAF
                     VSEDTGNPRTRGAFTLTWNKGPFEVAGTVNYISHYTVLDPSEGMADCADAIGAVGSVP
                     SSFCRVGSFTEFNLTARYDVNEHLQLHGSVINLFNRRAPYDLQTFGSPGNGAQQGGAP
                     YNPSFHQDGAVGTMFTIGGTYRF"
     gene            complement(9418..10281)
                     /locus_tag="VF453_13065"
     CDS             complement(9418..10281)
                     /locus_tag="VF453_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7688635.1"
                     /translation="MTTRRTLFGPGFARGFGRAWLVGGYLFLYLPMLTLVVYSFNHSR
                     LPSQWGGFSLEWYAKLAGDDELINGLKLSFKIALLSATGSVLVGTMAAFTLAKYPRFR
                     GRTVFSGMISAPLVMPEVIQGLSLLLMLVAIQRAIGFPERGLLTIWLGHMVIGISYAT
                     VVVQARLKTLNPQLEEAAMDLGARPWQVFTLVTLPMIAQALLSAWLLTFSLSLDDVVV
                     TAFLNGPGATPLPIVIFSRARLGLDPSVNAVATLIIFVVAVVVAAASIWVARNERRRA
                     AEISAAQRDES"
     gene            complement(10278..>11022)
                     /locus_tag="VF453_13070"
     CDS             complement(10278..>11022)
                     /locus_tag="VF453_13070"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7688636.1"
                     /translation="VLKYSNFVALTDDRLYIVAYLTSLKYALCTTLLCLAIGYPFAYF
                     MARAKPGVQPTLLMLVMLPFWTSFLLRVYAWKALLDEGGWIARLLIALHLDRVLAQVG
                     LIDVPGQLMHTPFSLIVGMTYTYLPFMVLPLYANLSKMDLRLLEAAADLGATPWVAFW
                     RVTVPLSFAGIVAGSMLVFIPCVGEYVTPELLGGPQTLMIGRVIWDENFSNNDPPMAA
                     AVAVVLVLLIVLPLALFNKYQSESQEARK"
BASE COUNT         1574 a         3808 c         3998 g         1642 t
ORIGIN      
        1 ggcgtcccgt gggctcgcgc gggcgctcgg tcaggccgac gccagcgcct cgtcgatctt
       61 gtccttgatc cgatgcagcc cgcgcagcag cgagccctgg tcgagcatgg cctggggggc
      121 gagcttcatc gcgacgtcct cgagcttgcc cggctccgtc acgagggtgt gggcgagggc
      181 ggacagcacg cagatgtatc ggtgcgagac ccgcggcagc cgcatcgtcg actggatctc
      241 gacgtgatgg cgcacgctgg cgaccgccgc gggcgcaagc ccccaggctt cgcacagccg
      301 cgcgccgact tcctcgtgcg tcgtggcgaa ggcctggcgc tcgagctcga gcagcgcctc
      361 gtcgttggcg gcctgcgcga gcatcggggc gtagcgcgcg gcgtcgtggc ggaagaggac
      421 ggccttgccg cattcctcga acagcccggc ggtgtgggcg ccccaggcct ccatcgacag
      481 cgcctgcgcg aggcggccca tcagcaggcc gcgcaggcgg gcgcgctccc agacggcgac
      541 cagctgcggc acctgcggga acgcggcctg caggccggtc tcgtaggcga tggccgagat
      601 ctcgcgcacg cccaggtagg tcacggcctg ctgcacgctc tgcacccgcc ccttgaggcc
      661 gtacagcggc gagttcacgg ccttcatcac ggccgcggcg aaggacatgt ccgaatcgat
      721 gagcgcgccg atctgttgga ggttgacgtc gtcctcgcgc agcagcgcgg ccagcttcac
      781 cagcgactgg ggctgggcgg ggaggtcgaa gtccaggttg actggtcgtt ggtccgtcat
      841 gggtgctccg gcggcagggc ccgccatccg cgggcaagtc gcatgctagc ccgcccgcgc
      901 ggtcgagcat gggcaaaatg accggaaatc accggccaaa ccgccgccga gggggcacgg
      961 gtcgcgattc ggcgccgatc cgggagagtg ggcgggcatg gcgcctgccg agcgggccgc
     1021 accagacaag ttgcgtggag agggagcttc gatgacggac tggccggcgc ccacggtgcc
     1081 gaacctggcg atctggacga tcgccgcgat cgcgactgcg ggcgtgatcc tgcgccccgg
     1141 gcgcgtgccg caggccgcat gggcgcttgc gggcgccgcg gccctggtgg tgacgggcct
     1201 gctgccgctc ccgtcggccc ttcgcgggct ggccgagagc gtcgatgtgg tcctcttcct
     1261 ggccgggatg atgctgctgt cggagctcgc gcggcgcgag ggcctgttcg acacgctcgc
     1321 cggctgggcg gtgcggcacg ccgacgggtc ggcgccgcgg ctgttcacgc tgatctacgg
     1381 cgtcggcgtg ctggtgacgg tgttcctctc caacgacgcg accgccgtcg tgctgacgcc
     1441 cgcggtctgc gtcgccgcgc ggcgcgcgaa ggcggcgcat ccgttgccct acctgttcgc
     1501 ctgcgccttc gtggccaatg ccgcgagctt cgtgctgccg atcgccaatc ccgccaacct
     1561 ggtcgtcttc ggaacccacc tgccgccgct ggacgcctgg tgccggcgct tcgcgctgcc
     1621 ttcgatcgcg gccatcgtgg cgacctgggc gctgctgcgg tgggcgatgc gggcgcggct
     1681 gcgcgagccg ctcgagcgcg tcgacgacgt gccgccgctc gacttcacgg gcctgctcgc
     1741 ggccgtgggc gtcgccggcg tggtgctcgc gatgctcgtg gcctcggccc tcggcgcggc
     1801 cctgggcctg ccgacgctgg ccgcgacgct gctcgcggtc ggcggcgtgc aatgggccaa
     1861 gcgcgagtcg cccctgccgc tgctcgaaca cgtcgcctgg aacgtgctcg tgatggtggc
     1921 cgggctgttc gtgatcgtcg agggcctggt gcgcaccggg ctcgtcggcg ccctggccga
     1981 ggcgctgcgc gacctggccg ggcactcgcc gcacgcggcc gcgtgggcgg cgggcatggt
     2041 cacgggcctc ggcggcaacc tgctgaacaa cctgccggcg gggctgctgg cgggcatggc
     2101 cgtgaatgcg ggccccgtcg acgacgccgt gcgctcggcg gtcgccatcg gcatcgacct
     2161 cgggcccaac ctgtccatca ccggctcgct ggcgaccatc ctctggctcg ccgcgatccg
     2221 gcgcgagggg gagcacgtga gcggagtcga gttcctgaag atgggggcga tcgtgatgcc
     2281 gctcgcgttg gtggtcacgc tggcgctcgt ctgagcgaga gcgcccgcac ggtcgcgcag
     2341 gtggtgcggg tcatacgggt atgggtacac ccgcggccgc accctccctc ccgggcgggc
     2401 cgccgccgag gactgcctgg cgaccgttca ccgcagccga tcgaccgttc agcgagccgg
     2461 cccttccccg cgtggcgggc gcggcgctga aatcgggccg gcccggcgtt gcgcacgtcg
     2521 atcgaccgcg cacgagctca cctggcgatg cgcggcctcg cgagactgca tcccggcgcc
     2581 gacagcgtcc gctggccggg cgaaccgggg ctaccgtcgc ccggtcgcgc gacatgccgg
     2641 cgtccgtcac aaccaacctg ggagattgca atgaaccaca aaccccgcgg cctgcgccgc
     2701 acggtctcgc tcgcgtccgc ggcactaggc ctgggcgcgg cggccgcgca agccgccacg
     2761 tcctatccct tcaacgacgg cgtcgtcgtc gccctgcagc ccgcgagcgg cacgacgatc
     2821 acggtcgcga cgccaaccgc cggcgcggcg ttccagggca ttggcaccaa cctcgggcag
     2881 ctcggcgaca tgggctacat cccctgcgcc caaggcagca ccggctgcac ggcggcgccc
     2941 ggaagcccga agatctcggc gaagtccgtg cgcgcctcga tcaacctgcc gttcaatgcc
     3001 cagggcagct ggactctcgt caaccaggtc tgcacgccgg tcacggtcac cgggctgggc
     3061 gtgaagttcg gcgatcccaa gggcacgctg ggcttcttcg acacctacgg cgccacgacg
     3121 ttctactcgg acgcccagtg cagcagcggc gcctcgatgc aggatcgctt cggcagcgcg
     3181 gccaactgca ccgacacctc gccggcgaac tatcgcggcg cctgcctcat ggacggcgtg
     3241 cgcatgcgca atctcgactg gctcgaggtc tacttcgccg cgcccgccga gtacatgcag
     3301 tcctgcggct ccggctgcga caagctgctc ggcgacaccg cgacgatgca gaacttcgcg
     3361 atgtacaagg cggcgcagct cgtctaccag ggccgcttcg gcagcgcgcc ggcggcgccg
     3421 aagtacgtcg agctgttcaa cgagcccgag ggcacgtgga acgtgcaggt cacgcctgcg
     3481 cagtacgacc agttcgtcgg ctacttcatg actgcgatgc agtcgctcgt cgcgcagcgg
     3541 ctgaagacgg cgggcctgtc gcaagccgag aagacctacc tcggcgacct gcagcacgtg
     3601 accgtcatgg cggggccggc gctcggctcg ggctacctgc tcgactacgt gggcgattcg
     3661 gcgccgtggg acacctccaa ggactggttc gccagcgcgg ccgaggcctc gagcttctac
     3721 gcgacgctgg cggccaacaa cgccgtcgtg tccacgcacc tgtacgacga caagcgcacg
     3781 ggcgcccagt tcaccggcct gaacgtgatg accggcctcg ggcagctcct ggcggtgcgc
     3841 aacgcgtcgt ccagcaacgc cgcggtcgtg atcagcgaag cggccgagac ggtgcagaac
     3901 gacgatgtcg cggtcaacgg ctaccgcggc ttctgcgacg cgaacacgcc gcgcaacgac
     3961 aagaagtcca ccgagggcgc gtgcgacgcg atgcggccgg gcgatcccgc gagcgtgggg
     4021 tactcgtatc ccgactgcat ccccggcgcg acctacgtga gcagcaccgc cggcaagtac
     4081 gcgggtccgt accagggcac ggcgctggcc gacttctgcc accaccaggc ggccaagctc
     4141 tatcgcaact tggccaacta cgcgaaccag aacgaccagc atgccaccag cgtcaacggc
     4201 gtcgtctccg agctgatctg ggcctcctcg ccgacgccct cgagctccga gcacgggctg
     4261 gtcacgcgcc aggggctgcc gtcgctgatg aacgtcggca ccgatccgct gctcgccacc
     4321 ttctccgccg acggctatgc gacggtcttc ccggtcacgt tcgcgcagga cttcgacctc
     4381 ggcgcgggga tcttcgcctc cgcggcgacg aacccgcagc gggctcgcct cgcgctggcc
     4441 aataccgcgt cggtgaccaa gacggtcacg ctgaacttcg gcagcacgcc gctgcggcag
     4501 ttcacgcgcg tgctggacgg cagctcgttc ggcggtaccc gggacaagta cacgggccag
     4561 ctcgagtcca tggcctcggg gctgcaggcg cccgccggca acaacttcgg cagcaggaac
     4621 tacaacctcg tcgtcacgct gccggccaat tcggcggtga cgctgaccct cgcgccctga
     4681 cggaacgacc ggccgcgcga aaggggccgg tcgcgccggc cccttctcat cggatcagcg
     4741 cgtcacttcg ccccgtcgat ccggatccac gtcgtcttca gctcggtgta cttgtcgaag
     4801 gcgtgcagcg acttgtcgcg cccgttgccg ctctgcttgt agccgccgaa ggggacggtg
     4861 atgtcgtcct cgtcgtagga gttgacgtgg atcgtgccgg cgcgcagcgc gcgcgcgacg
     4921 cgatgggcgc ggttgatgtc gtcgctccag acgctgccct gcagaccgta gctcgagccg
     4981 ttggccaggg cgacggcctc gcgctcgtcc ttgaagcgga tcaccgacat caccgggccg
     5041 aagatctcct cctgcgcgat cttcatcgag ttggcgacgc cgtcgaacac cgtcggctcg
     5101 acgtagtagc cgccggtctc ggcgcgcgcc tggcggccgc ccgcgacgac gcgcgcgccc
     5161 tcggcgtggc cggcgtcgat gtagccgagc acggtgcgca gctgcgtgtc gtcgacgagc
     5221 gcgcccatct cggtcttcgc gtcgagcggg tcggcgggcg cgtatttcgg cgcttcggcg
     5281 gcgacgatct tcacgaactc gtcggccacg cgctcgtgca cgagcacgcg cgagggcgcg
     5341 ttgcaggatt cgccctggtt gaagaacatc gacccggcga ccgtcctggc ggcgcgctcg
     5401 agatccttga agtcgtcgaa cacgacgaac gccgacttgc cgccgagctc gttgtagacg
     5461 cgcttgaggt tggacttgcc ggcgtactcg tacatgcggc ggccgacgcg ggtcgagccg
     5521 gtgaagccga tggcgtcgac ctccatgtgc agcgccagcg cctcgcccgc ctcgtgcccg
     5581 tagcccggca cgacgttgaa gacgcccggc ggcaccccgg cttcgagcgc gagctcggcc
     5641 aggcgcagcg cggtcagcgg gctcttctcg ctcggcttga gcacgacgct gttgccggcc
     5701 gcgagcgccg ggcccagctt ccacgcggcc atgatcatcg ggtagttcca cggcacgatg
     5761 acgccgatca cgcccatcgg ctcgcgcgtg atcagcgcca gcgcgttcgc cgcggtcggc
     5821 gcgatctcgt cgtagacctt gtcgaccgcc tcggcatacc aggcgatgca gcgtgcgcag
     5881 gcgggcacgt cgacggcgag cgagtactgg atcggcttgc ccatgtccag ggtctccagc
     5941 agggcgagct cgtccttcgc ggcgaggatc ttgtcggaga agcgctgcag gatcttcttg
     6001 cgctgcgcgg gcgccttgcc ggcccagcga ccgtcctcga acgcggcgcg cgccgcgcgc
     6061 acggcgaggt cgacgtcggc cgcgacgccg cgcgcgatcg gcccgagcag gcgggcgtcg
     6121 atcggcgaat gcttggcgaa ggtttcgccc gactgggtgg cgcggcgctc gccgtcgacg
     6181 agcatgcggc cgtcgatgcg cagggcgccg gcgcgctcgg cccagctggt cgcggcggga
     6241 gtgggagtgg tcatgcgagg tctcctggtg ggatgcgggg gggcggcggc tcgtggccgc
     6301 ggccgtcgtg ggcggtgcgc cgcggtgatc cgccgcggcg cacgcgaagt cgccagtatg
     6361 ccggtcgtgc gcggccggcg gacgcgaccg ggacatgccg accgccgcga ctgcgcgcgg
     6421 ctcggagcgg ccgcggcccg gtctcgcgtc agaagcggta ggtgccgccg atcgtgaaca
     6481 tcgtgccgac cgcaccgtcc tggtggaagg acgggttgta cggcgcgccg ccctgctgcg
     6541 cgccgttgcc gggcgagccg aaggtctgca ggtcgtacgg ggcgcggcgg ttgaacaggt
     6601 tgatcacgct gccgtgcagc tgcaggtgct cgttgacgtc gtagcgcgcg gtcaggttga
     6661 actcggtgaa cgagccgacc cggcagaagc tcgacggcac gctgcccacg gcgccgatcg
     6721 cgtcggcgca gtcggccatg ccctcgctcg ggtcgagcac ggtgtagtgc gagatgtagt
     6781 tgaccgtgcc cgcgacctcg aacgggccct tgttccaggt cagcgtgaac gcgccgcgcg
     6841 tgcgcgggtt gccggtgtcc tccgacacga aggccgggcc gtgcgtgccg gcgaggtggt
     6901 acttcacgcc ctgcgcggtg aggtcgtagg cgagggtgta gctcacctgc agctcggcct
     6961 tcaggctgcc ggcggcgccc aggccgtact tgcccaggac gtcgaggtcc aggccggacg
     7021 tcgtcgtcgt gttcgcgttg atgaaggggt aggtgtcgta gaggatgttc tgcgacgagt
     7081 cgcggtagat caccgtgccg agcgcggccg cgtcgtcgag gccgatctgg cccagctggc
     7141 cgacggagat gatctggttg cggatctgga tgtggtagta gtccagcgtc gccgagtagc
     7201 tcttgctcgg ctcgaggatc aggcccagcg tgaaggactg cgacttctcg ggcttcaggt
     7261 gcggcgagga cagctgcaac tcctgcagcg cgatgttgca ggggtcgagc atgccggcgg
     7321 gcaggttcgc ggccggcgtg ccacccgggc agtagaccgg gtcgtgcagg ttgccgacga
     7381 agcccgaggt cgagcccgcg tcgcctgtct cggccgggtt gggggcgcgg aagcccttgc
     7441 tgtaggtgcc gcgcagcgtg aactcgcgcg ccggcgacca cttgaagccg gccttgggcg
     7501 tggccgaggt gccgtaggtg ttgtagtggt cgacgcgccc ggccaggtcg atctccagct
     7561 gcttgctgac cggcgccacc agctcggcgt aggtcgcggc caccgtttgc gaaccctggg
     7621 cgaacgagta gatcccgatc gcctgcgcgc cgttgacgaa cgacggcggg aagatctcgt
     7681 gcagtcgccg gtgcgtgagg tcgacgccca ggcccagcgc gagcgggccg cccggcaggt
     7741 cggtcagcgt ccgggtgccg cgggcggtga ggaagctcag ctcgttcgtc gagaccgagg
     7801 acgcgtgcgg cgccaccgtc gacaggatcg ccgagttgtc gccaccgacc tggtacgtgc
     7861 cgtcgtccag cgcctgctgc atcgcgggga agtcgatgta gtggtcgaag ctcgcgcgcg
     7921 tgacggcgcg cgtcacgccg ccggagaagt tcaggtccca ctcgccgacc gagccctgcg
     7981 cctccgccac ggtacggtag gaggtggtga cgacggtctg ctcgagcgga ccgatgtcga
     8041 ggaagtcgta gaccagcggc gcgtcggtgc cggtcgggtt gcccgggtag ttggcgggca
     8101 ccgtcagcac gaacgggaac tggtcgggga aggcgggcgc cggcgcgctg ccggggccga
     8161 agtggaacag gttgacgccg ccgccgttgc cgcccgagcc gacgccggtc gcggcggcgt
     8221 tgttgaacag gccgacctgg tgcgcctcgc tgcgcagcgc ggagccctgc aggttggcct
     8281 cccagccgcc gcccagcttg gtggtgtagc ggccgagcag gtcgatgttg gtcgtcgagg
     8341 gctgcagcgt ctggtacggg ttgctgtagc cgcaggtgtc ggccgccgcc tgggcgtccg
     8401 tgcagccggg gaagtagtag accgggttgc cgctcgagtc ggtgagatag cccgtgccgc
     8461 tgcccgcgcc gctggaggcc cccgagaccg cgcccgagcc cgtgccctgc gtgaggtcga
     8521 cgccgccgta cggccgccag tccgtccgcg ccaggtaggc ccgcttcctc agcaggatgt
     8581 cctgctggtg gcgtgcctcg agcgcgacgt aggcgttgtg gccgtccttc gcgagatcgc
     8641 cccaaccggt gatgaagctg ccgtgggtgg tcgcgccgtc accgtggctc gtgacgccgc
     8701 cctcggcgct gaccgtggtg ccggtgaagc tcttcttcag gatcacgttg accacgcccg
     8761 cgatcgcgtc cgagccgtag accgccgacg cgccgtcctt gagcacctcg atgcgctcga
     8821 tcgcctccat cgggatcgag gagatgtcga cgaagtcgcg ctcgccgtcg tcgggcatcg
     8881 ggtaggaggc catgcggtgg ccgtcgatca gcaccagggt cgcgcccacc gtcaggccgc
     8941 gcagggcgac gccgctgccg cccgccgcga acgcgctggg cgccgactgc gacagcgagc
     9001 ccatgttgtt cgcggtgatc gagtgcaaca cgtcgccgac gctggtgtag ccggagttgc
     9061 ggatgtcctc ggccgacagc acctgcaccg ggctgggcgt ctccgagtcc gtgcggcgca
     9121 cgttcgagcc ggtgacggtc acctgctgca ccgccggcgc aggcgcggca tcctgggcat
     9181 gggcctgcag gcaggccacc tgcatcgccg cgacgatcgc ggccggtcgc cacgcgggcg
     9241 gggcgttctt cttgcgagac atggcacttc ctccaggttg tggaaaagac aggcacggca
     9301 cgacccaccg gggccgcacg ggggccccgg atcgggcggg acatttcgtc gctctcaggg
     9361 cccgcgagcg aaagccgcgc aggccggggg ggaagccgct ctcgtgaagc ggatccctca
     9421 ggattcgtcg cgctgggccg cgctgatctc ggccgctcgg cggcgctcgt tgcgggcgac
     9481 ccagatgctc gccgccgcga cgacgaccgc gacgacgaag atgatcagcg tggccaccgc
     9541 gttcacgctc ggatccaggc cgaggcgtgc gcgcgagaag atgacgatgg gcagcggcgt
     9601 ggcgcccggg ccgttgagga aggccgtgac gacgacgtcg tccagcgaca ggctgaaggt
     9661 cagcagccag gcggacagca gcgcctgcgc gatcatcggc agcgtcacca gcgtgaacac
     9721 ctgccacggc cgcgcgccga ggtccatcgc ggcttcctcc agctgcggat tgagggtctt
     9781 cagccgcgcc tgcacgacca ccgtcgcata cgagatgccg atgaccatgt ggcccagcca
     9841 gatcgtcagc aggccgcgct cggggaagcc gatcgcgcgc tggatcgcca ccagcatcag
     9901 gagcagcgac agcccctgga tcacctcggg catcaccagc ggcgcgctga tcatgccgga
     9961 gaagacggtg cggccgcgga agcgggggta cttcgcgagc gtgaaggccg ccatcgtgcc
    10021 gaccagcacc gagccggtgg ccgacagcag cgcgatcttg aacgacagct tcaggccgtt
    10081 gatcagctcg tcgtcgccgg cgagcttggc gtaccactcg agcgagaacc cgccccactg
    10141 cgacggcagc cgcgagtggt tgaacgagta cacgacgagc gtgagcatcg gcaggtacag
    10201 gaacaggtag ccgcccacga gccatgcacg gccgaagccg cgcgcgaagc cggggccgaa
    10261 gagggtgcgc ctcgtcgtca tttccgcgcc tcctgcgatt cgctctggta cttgttgaac
    10321 agcgccagcg gcagcacgat cagcagcacc agcacgaccg ccaccgccgc ggccatcggc
    10381 gggtcgttgt tgctgaagtt ctcgtcccag atcacgcggc cgatcatcag cgtctgcggg
    10441 ccgccgagca gttcgggcgt cacgtactcg ccgacgcagg ggatgaacac gagcatcgag
    10501 cccgcgacga tgcccgcgaa cgacagcggc accgtcacgc gccagaacgc gacccagggc
    10561 gtcgcgccga ggtcggcggc ggcctcgagc aggcgcaggt ccatcttcga gaggttggcg
    10621 tacagcggca gcaccatgaa gggcaggtag gtgtaggtca tgcccacgat cagcgagaag
    10681 ggcgtgtgca tcagctggcc gggcacgtcg atcaggccga cctgcgcgag caccctgtcc
    10741 aggtgcagcg cgatcaggag ccgggcgatc cagccgcctt cgtcgagcag cgccttccac
    10801 gcgtacacgc gcagcaggaa cgaggtccag aacggcagca tcacgagcat cagcagcgtg
    10861 ggctgcacgc cgggcttcgc ccgcgccatg aaatacgcga acgggtatcc gatcgcgagg
    10921 cacagcagcg tcgtgcacag cgcgtacttc aggctcgtca ggtaggcgac gatgtagagc
    10981 ctgtcgtcgg tcagcgcgac gaagttcgag tacttcagga cg
//
LOCUS       DASVLN010000541         7158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_513806, whole genome shotgun sequence.
ACCESSION   DASVLN010000541 DASVLN010000000
VERSION     DASVLN010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513806"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..2064
                     /locus_tag="VF453_13075"
     CDS             319..2064
                     /locus_tag="VF453_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7688637.1"
                     /translation="MATPPNDPGTTPAADARKQAQNRDHQTFPVLDAVDMARIVRFGT
                     HERYAKGEALIRAGQPTRGMFLVTAGRGEVLQRDGLGHVVPLRTYGPGEFLAEAGSLT
                     GARVLVDVVALEDIEGWLVRPDQLRALIIAEADLGERIMRALILRRVALIQAGASGAT
                     LIGAAGSASILRLQNFLRRNGQPHHVVAAEDDPAAAALLVQYGAAQAEAVAVCPDGTV
                     LVEPSDRELAVALGMIDDRQCEALFDVLVVGAGPAGLSTAVYAASEGLQVAVIDCRSF
                     GGQAGASARIENYFGFPTGISGQALAGRAFIQAQKFGAKIIIPAEATALDCSRAESEG
                     LFIVKLADGRTLRSRSLVIASGARYRRPALPCIAEYEGRGVWYWASALEARLCGGEEV
                     VLVGGGNSAGQAAVFLSGHASQVHMLVRGEGLAASMSRYLIDRIEAAPNIRLWTRTQL
                     AGLGGDLEAGVRRARWRCGADDSLHERELRHVFLFVGADPETRWLEGCGVAVDRSGFV
                     LTGVDAITGPDGRARPGRVPNPFETSVRGAFAVGDVRAGSVKRVGGAIGDGAAAVALI
                     HQHLAALAARTAVVA"
     gene            2211..3092
                     /locus_tag="VF453_13080"
     CDS             2211..3092
                     /locus_tag="VF453_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7688638.1"
                     /translation="MTHVNVPQTFRIAGELEIRRLGFGAMRITGPGIWGPPADRDEAL
                     RVLRRLPELGVDFIDTANSYGPNVSEELIREALHPYTDVTVATKGGLERPGPDQWSPR
                     GDPVYLVNEAVASRDRLDVGQITLWQLHRIDPKVPRDEQFDAVRQLVTDGVIEHAGLS
                     EVSVADIEAASKFFRVASVQNRYNFADRGSEDVLEYCEKHGIAFIPWFPLGAGELARA
                     GEKLDAIASAHGATPSQVAIAWLLQRSPVIVPIPGTSKVAHLEENVTARDIVLSDEEF
                     ATLDEMGKPKGLGHGRD"
     gene            complement(3098..4000)
                     /locus_tag="VF453_13085"
     CDS             complement(3098..4000)
                     /locus_tag="VF453_13085"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEX7688639.1"
                     /translation="MTANTPLDRWRGALLGLACGDAVGTSVEFCARGSFDPVTDMSGG
                     GPFALAPGEWTDDTSMALCLAASLVHRRDHDPVDQMNRYVNWWRYGYFSATGECFDIG
                     MTVQRALRRYLDTGEPYAGDPDPRTAGNGALMRLAPVAMWFAADPAALRDAARASTRT
                     THAAPEALDCSELFAELLRRALVGAPKAALLSGPAPAGCTPAVEALARGDWRDKPADA
                     IHGSGYCVESLEAALWCFATTTDFESAVLAAANLGDDADTTAAIVGQLAGAHHGADAI
                     PPRWRERLAMADEIERLARALHAR"
     gene            complement(4011..4736)
                     /locus_tag="VF453_13090"
     CDS             complement(4011..4736)
                     /locus_tag="VF453_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7688640.1"
                     /translation="MPARPPAARNDFPRPFTTVDLVIFTVLDEALKVLLVQRPSGPGE
                     PFPGAWALPGGFVDVERDADLLACARRKLREKTGVATPYLEQLGSWGGAARDPRGWSA
                     THAYFALMPAAGVVLEKGANAADVAWFAVDELPARRKLAFDHAQILQAGVERLRGKVE
                     YTSLPAFLLPEPFTLPQLQRTYEIVLGRPVDKSGFRTRMMAAGFLVETGTVESDSNRP
                     PMGYRLKDRSAPVFFPRTFSPRG"
     gene            4848..5411
                     /locus_tag="VF453_13095"
     CDS             4848..5411
                     /locus_tag="VF453_13095"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003950 - NAD+ ADP-ribosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HEX7688641.1"
                     /translation="MTHENTTDKATVRRSKFLSLVLRHEPQRIGIALDQAGWVAVDEL
                     LARAAAAGRTMTRAQLDEVVATSDKRRFAFSEDGLRIRASQGHSVAVDLGLPPREPPP
                     ELWHGTASRNIASILRAGLDKRARHHVHLTANRATAIAVGARYGVPVLLRVAAAAMAA
                     DGHVFHCSDNGVWLVDAVPAKYLEVVA"
     gene            5408..6286
                     /locus_tag="VF453_13100"
     CDS             5408..6286
                     /locus_tag="VF453_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HEX7688642.1"
                     /translation="MRAARDGRAALARETLAIVDAGRYVAPDGRPVDIGDAVRACVAS
                     TYLVLPEAREALAARVAAIGAFDADVAVELTAETTLAAARRLAGRGFACVGVLNFASA
                     RHPGGGFLGGSQAQEESLARSSALHASLTSPAAEPYYTHHRAERSLLYTDRVVVSPAC
                     PVVRDDDGTLLAEPHEPTFLTCAAPNAGAIQANQPESLARLPEVFARRMDGVLAAAVD
                     AGCDALVLGAWGCGVFRNDPALVAALFRAWLAGGGPYARRFRHVCFAVFESSADKPCL
                     KAFERAFGGPKSAPFE"
     gene            complement(6317..>7158)
                     /locus_tag="VF453_13105"
     CDS             complement(6317..>7158)
                     /locus_tag="VF453_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7688643.1"
                     /translation="GAPTCIVAAPLLAGSRAAGGLGLVLDAKPRLESILRQVLPEASG
                     AVAAFCRSDGVVIARTGELPVALPASVLALAPGQAWSGVLVEDGRCFVVGAAALAQAL
                     GTGPAPGGAALVGVVIVPCGTPVAPAVDALPEIPPVDDGAEIATFLVGHRLLGVPAEA
                     VVECIEVAAAVRVWRGGFAQRHAGYVTWNDAALPLVDIAADVEAGPGAVHRHAIVMRS
                     GGQDFGLLVSELGPVARMQLSEERGLAGGGDASRLIAQLARAGSVLLPVLAPEAVFGM
                     GRG"
BASE COUNT          959 a         2666 c         2614 g          919 t
ORIGIN      
        1 cggcggccgg ccccgaggcg agcacggggc gccaggccag cgcggccagg ccggcggcga
       61 gcgcggtgcg gcggttcacg gccgggacgt gggcgggcgg ggtggtggcg tacagggggg
      121 gcatcggcgt gctctgcttc ctggcggtgg gcgcggacgg cgcgcgactg cctgttcaac
      181 ggccgcgccg cgccgatgtt gacaccgctc gcgttgctgc cgtgcaaagt cgtgatgccg
      241 caaagttgtc gtccgacggt ggcgagattt cgcttaggct tcgcgtcagc tcgcaaccat
      301 cccgacggga gaccgaccgt ggccacgcct ccgaacgatc cgggcacgac gcccgccgcc
      361 gacgcccgca agcaggccca gaaccgcgac caccagacct tcccggtgct cgacgccgtc
      421 gacatggccc gcatcgtgcg cttcggcacg cacgagcgct acgccaaggg cgaggcgctg
      481 atccgcgccg gccagccgac gcgcggcatg ttcctcgtca ccgcgggccg cggcgaggtg
      541 ctgcagcgcg acggcctcgg ccacgtcgtg ccgctgcgca cgtacggccc gggcgagttc
      601 ctcgccgagg ccggcagcct gaccggcgcg cgcgtgctgg tggatgtcgt ggcgctcgag
      661 gacatcgagg gctggctggt gcgccccgac cagctgcgcg cgctcatcat cgcggaggcc
      721 gacctcggcg agcgcatcat gcgggcgctg atcctgcgcc gggtcgcgct gatccaggcc
      781 ggcgcgagcg gcgcgacgct gatcggcgcc gcgggctcgg cctccatcct gcggctgcag
      841 aacttcctgc gccgcaacgg ccagccgcac cacgtcgtcg cggccgagga cgatcccgcc
      901 gccgcggcgt tgctcgtgca gtacggcgcc gcgcaggccg aggccgtggc cgtctgcccg
      961 gacggcacgg tgctggtcga gccgtccgac cgcgagctgg cggtcgcgct gggcatgatc
     1021 gacgaccgcc agtgcgaggc attgttcgac gtgctggtcg tcggcgccgg cccggcgggc
     1081 ctgtcgacgg cggtgtacgc ggcctccgag ggcctgcagg tcgccgtgat cgactgccgc
     1141 tcgttcggcg gccaggccgg cgccagcgcg cgcatcgaga actacttcgg cttcccgacc
     1201 ggcatctcgg gccaggcgct ggccgggcgc gcgttcatcc aggcgcagaa gttcggcgcc
     1261 aagatcatca tcccggccga ggcgacggcg ctggactgct cgcgcgccga gagcgagggc
     1321 ctgttcatcg tgaagctggc cgacggccgc acgctgcgca gccgcagcct cgtcatcgcc
     1381 agcggcgcgc gctaccgccg cccggcgctg ccctgcatcg ccgagtacga gggtcgcggc
     1441 gtctggtact gggcctcggc gctcgaggcg cggctgtgcg gcggcgagga ggtcgtgctc
     1501 gtgggcggcg gcaactcggc ggggcaggcc gcggtgttcc tgtccgggca cgcgtcgcag
     1561 gtgcacatgc tggtgcgcgg cgagggcctg gccgccagca tgtcgcgcta cctgatcgac
     1621 cgcatcgagg ccgcgcccaa catccgcctg tggacgcgca cgcagctcgc cggcctgggc
     1681 ggcgacctcg aggccggcgt gcgccgcgcg cgctggcgct gcggcgccga cgattccctg
     1741 cacgagcgcg agctgcgcca cgtcttcctg ttcgtcggcg ccgatccgga gacgcgctgg
     1801 ctcgagggct gcggcgtcgc ggtcgaccgc agcggcttcg tgctcaccgg cgtcgacgcc
     1861 atcacgggcc cggacggccg cgcgcgcccg ggccgcgtgc ccaacccgtt cgagaccagc
     1921 gtgcgcggcg cgttcgcggt cggcgacgtg cgcgcgggct cggtcaagcg cgtgggcggg
     1981 gcgatcggcg acggcgcggc cgcagtggcg ctgatccacc agcacctcgc ggcgctggcg
     2041 gcccggacgg ccgtggtggc ctgacgaccc gtcacatccg cgccacgcgg cggtcgcatc
     2101 gtgggccaag accctgcggc cggcgctggc gcatgccttg ccgatactgg ggccgatcgc
     2161 cgcgcgccgc ttcctgcgcg gccgcgccat tccatgcccc ggagattccc atgacgcacg
     2221 tcaatgtccc ccagaccttc cgcatcgccg gcgagctcga gatccggcgc ctcggcttcg
     2281 gcgcgatgcg catcaccggc ccgggcatct ggggcccgcc ggccgaccgc gacgaggccc
     2341 tgcgcgtgct gcgccgcctg cccgagctgg gcgtcgactt catcgacacc gccaactcct
     2401 acggcccgaa cgtctccgag gagctcatcc gcgaggcgct gcatccctac accgacgtga
     2461 cggtcgcgac caagggcggc ctggagcgcc cgggccccga ccagtggtcg ccgcgcggcg
     2521 acccggtcta cctcgtcaac gaggccgtcg ccagccgcga ccgcctggac gtcggccaga
     2581 tcacgctgtg gcagttgcac cgcatcgacc ccaaggtgcc gcgcgacgag cagttcgacg
     2641 cggtgcgcca gctggtcacc gacggcgtga tcgagcacgc cggcctgagc gaggtgtcgg
     2701 tcgcggacat cgaggcggcc tcgaagttct tccgcgtcgc gagcgtgcag aaccgctaca
     2761 acttcgccga ccgcggcagc gaggacgtgc tggagtactg cgagaagcac ggcatcgcgt
     2821 tcatcccgtg gttcccgctc ggcgcgggcg agctcgcgcg cgcgggcgag aagctcgacg
     2881 ccatcgcctc ggcccacggc gcgacgccca gccaggtcgc gatcgcctgg ctgctgcaac
     2941 gcagcccggt catcgtgccg atcccgggca cctcgaaggt cgcgcacctg gaggagaacg
     3001 tcaccgcacg cgacatcgtg ctgagcgacg aggagttcgc gacgctggac gagatgggca
     3061 agcccaaggg gctgggccac gggcgggact gaagcgctca gcgcgcgtgt agggcgcggg
     3121 cgaggcgctc gatctcgtcc gccatcgcga gccgttcccg ccagcgcgga gggatcgcgt
     3181 cggcgccatg gtgcgcgccg gcgagctgcc cgacgatggc ggcggtcgtg tcggcgtcgt
     3241 cgcccaggtt ggcggccgcg aggacggccg attcgaagtc ggtcgtcgtg gcgaagcacc
     3301 acagggcggc ttcgagcgac tcgacgcaat atccgctgcc gtggatcgca tcggcaggct
     3361 tgtcgcgcca gtcgccgcgc gccagggcct cgacggccgg cgtgcagccc gccggcgcgg
     3421 gccccgacag aagcgcggcc ttgggcgcgc ctaccagtgc acgacgcagc aactccgcga
     3481 acagctcgct gcagtccagc gcctcgggcg cggcgtgggt cgtccgcgtg ctcgcgcgcg
     3541 cggcgtcgcg cagcgcggcc gggtccgccg cgaaccacat cgcgaccggc gcgagccgca
     3601 tcaacgcgcc gttgccggcg gtgcgaggat cgggatcgcc ggcgtacggc tccccggtat
     3661 cgagataccg ccgcagtgcg cgctgcaccg tcatgccgat gtcgaagcat tcgcctgtcg
     3721 cgctgaagta gccgtagcgc caccagttca cgtagcggtt catctggtcg acgggatcgt
     3781 ggtcacgacg atggaccagg ctcgccgcga ggcagagcgc catcgaggtg tcgtctgtcc
     3841 actcgccggg cgcaagcgcg aacggcccgc cgccgctcat gtccgtcacc gggtcgaagg
     3901 agccgcgcgc gcagaactcc accgacgtgc cgacggcatc tccgcacgcg aggcccagga
     3961 gcgctccgcg ccagcggtcg agaggagtgt tcgcggtcat ggagctggcc tcaccctcgc
     4021 gggctgaagg tgcgcgggaa gaacaccggc gcgctgcgat ccttcaggcg atagcccatc
     4081 ggcgggcggt tggagtcgct ttccaccgtg ccggtctcga cgaggaagcc ggccgccatc
     4141 atgcgcgtgc ggaagccgct cttgtcgacc gggcggccca gcacgatctc gtaagtgcgc
     4201 tgcagctgcg gcagcgtgaa cggctcgggc aacaggaagg ccggcagcga ggtgtattcg
     4261 accttgccgc gcaagcgctc gacgccggcc tgcaggatct gcgcgtggtc gaacgcgagc
     4321 tttcggcgcg cgggcagctc gtcgaccgcg aaccaggcga cgtcggcggc gttcgcaccc
     4381 ttctcgagca cgacgccggc ggccggcatc agcgcgaaat aggcgtgcgt ggccgaccag
     4441 ccgcgcgggt cgcgcgccgc gccgccccag ctcccgagct gttcgaggta gggcgtcgcg
     4501 acgccggtct tctcgcgcag cttgcggcgc gcgcaggcga gcaggtcggc gtcgcgctcg
     4561 acgtccacga agccgcccgg cagcgcccac gcgccgggga aaggctcgcc gggccccgag
     4621 ggccgctgca ccagcaggac cttcagcgcc tcgtccagca ccgtgaagat gacgaggtcg
     4681 accgtcgtga acgggcgggg gaagtcgttg cgggcggcag gcgggcgggc gggcatggcg
     4741 gggtcgggcg cttgacttcg tggtcgatgg gttgtactgt acacctttat tggatgcaca
     4801 gaacaactta tcccgggaca aagacaacga atgaacaggg agatcccatg acgcacgaga
     4861 acaccaccga caaggcgacc gtccgccgca gcaagttcct cagcctcgtg ctgcgccacg
     4921 agccgcagcg gatcggcatc gcgctcgacc aggccggctg ggtggccgtc gacgagctgc
     4981 tggcccgggc cgcggccgcc ggccggacga tgacgcgcgc gcaactcgac gaggtggtcg
     5041 cgacgagcga caagcgccgc ttcgccttca gcgaggacgg cctgcgcatc cgcgccagcc
     5101 agggccactc cgtggcggtc gacctcggcc tgccgccgcg cgagccgcca cccgaactgt
     5161 ggcacggcac cgccagccgc aacatcgcgt ccattctgcg cgcaggcctg gacaaacgcg
     5221 cccgtcacca cgtgcacctc accgcgaacc gcgccaccgc gatcgccgtc ggcgcgcgct
     5281 acggcgtgcc cgtgctgctg cgcgtggccg ccgccgccat ggcggccgac ggccacgtct
     5341 ttcactgctc ggacaacggc gtgtggctgg ttgacgcggt gccggcgaag tacctcgagg
     5401 tggtcgcatg agagccgccc gcgacggccg cgccgcgctg gcacgggaga cgctcgcgat
     5461 cgtcgacgcc ggccgctacg tcgcgccgga cggccggccg gtcgacatcg gcgatgcggt
     5521 tcgggcctgc gtcgcctcga catacctggt gctgcccgaa gcccgcgagg cgctcgcggc
     5581 gcgcgtcgcc gccatcgggg cgttcgatgc tgacgtcgcg gtcgagttga cggccgaaac
     5641 cacgctggcc gccgcgcgcc gcctggccgg ccggggcttc gcctgcgtcg gcgtgctgaa
     5701 cttcgcttcg gcccgccatc ccggaggcgg cttcctcggc ggcagtcagg cgcaggagga
     5761 atctctcgca cgcagctcgg cgcttcacgc atcgctcacg agtcccgcgg ccgagcctta
     5821 ctacacgcac catcgtgccg agcgctcgct gctctacacc gaccgcgtgg tcgtttcgcc
     5881 ggcctgcccg gtcgtgcgtg acgacgacgg cacgctgctc gccgagcctc atgagccgac
     5941 cttcctgacg tgcgccgcgc cgaacgcggg ggcgatccaa gcgaaccagc cggaatccct
     6001 ggcccggctg cccgaggtct tcgcacgtcg catggacggc gtgctcgcag cggccgtcga
     6061 cgcgggctgc gacgcgctcg tgctcggcgc ctggggctgc ggcgtgttcc gcaacgaccc
     6121 ggcgctcgtc gccgcgctgt tccgggcgtg gctcgccggc ggcggcccct atgcgcgccg
     6181 tttccggcac gtgtgcttcg cggtgttcga gtcgagcgcg gacaagccgt gcctgaaggc
     6241 gttcgagcgc gcgttcggcg gcccgaagtc tgcgccgttc gagtgacgcc ggctccgctt
     6301 gcgcgcggcg gtcgactcaa ccccgcccca tcccgaacac cgcctccggc gccagcaccg
     6361 gcagcagcac cgagcccgcg cgcgcgagct gcgcgatcag gcgcgaggcg tctccgccgc
     6421 cggccaggcc gcgttcctcg gacagctgca tccgcgcgac cgggccgagc tccgagacca
     6481 gcaggccgaa gtcctggccg ccggagcgca tcacgatggc gtggcgatgc acggcgcccg
     6541 ggccggcctc gacgtccgcc gcgatgtcca cgagcggcag cgcggcatcg ttccacgtga
     6601 cgtagcccgc atggcgctgc gcgaagccgc cgcgccagac gcgcaccgcc gccgcgacct
     6661 cgatgcactc cacgaccgcc tccgcgggca cgcccagcag ccggtggccg acgaggaagg
     6721 tcgcgatctc ggcgccgtcg tcgaccggag ggatctccgg cagcgcgtcg accgccggcg
     6781 cgaccggcgt gccgcagggc acgatcacca cgccgacaag cgccgcgcca cccggcgcag
     6841 gtcccgtgcc gagcgcctgc gccagcgccg ccgcgccgac cacgaaacag cggccgtcct
     6901 cgaccaggac gcccgaccac gcctgccccg gcgccagggc caggaccgac gcgggcagcg
     6961 cgacgggcag ctcgccggtg cgcgcgatca ccacgccatc ggatctgcag aacgccgcga
     7021 cggcgcccga ggcctccggc agcacctggc gcaggatgga ctcgagccgc ggcttcgcat
     7081 ccagcacgag gccgaggccg cccgcggcgc gactgcctgc cagcagcggc gctgccacga
     7141 tgcaggtcgg cgcgccgt
//
LOCUS       DASVLN010000542         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_513864, whole genome shotgun sequence.
ACCESSION   DASVLN010000542 DASVLN010000000
VERSION     DASVLN010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513864"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..405)
                     /locus_tag="VF453_13110"
     CDS             complement(205..405)
                     /locus_tag="VF453_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688644.1"
                     /translation="MNSIAASHTGRRSCAPPSVRAPRLVFVEDLNWPVPGAPTHRRPA
                     RMPEVRAPGRDGPWADVPLTRQ"
     gene            complement(477..671)
                     /locus_tag="VF453_13115"
     CDS             complement(477..671)
                     /locus_tag="VF453_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688645.1"
                     /translation="MSQQNQSKQDKSPSQQDQSSRSSSTRDSNAPQSGRNLRDDDQSR
                     SGSGSIDQQSGRQQQKGDRR"
     gene            complement(815..>2924)
                     /locus_tag="VF453_13120"
     CDS             complement(815..>2924)
                     /locus_tag="VF453_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7688646.1"
                     /translation="AAAPASAASVAGRAAVDAPSAGPIAAVQNVVVDGVRQRLDAARN
                     GLSPDTGTSQYKFSDADIAALPLGEATPLNQVVLQSPGVVQDSYGQLHVRGDHANVQY
                     RIDGVVIPEAISGFGQSLETRFADNIAILTGALPAQYGYRTAAVVDIRTKAQPAGDSG
                     SVSWTGGSNAHEEGSGEIGGTHGAVSYYLTGSLLRDGLGIENPTASKDALHDTTRQAK
                     GFGTVSYVLDDDDRLNLMFGTSNNRFQLPDVPGQAPSYSLAGASGIDSAALDARQRER
                     NQFEVLSWQGHAGQADGGVDYQLSYFHRATDVRYTPDPVGDLVFDGIAAEILRRNTAD
                     GLQFDGSVALGRAHTLRGGFFAQRERFVVDNSSAVFPADADGHQTSDVPETIVDDTAI
                     HGRLWGLYLQDEWQPAKGLTINYGARFDDVDTVVREHQLSPRLGLTWDATPTLRVHAG
                     YARYFTPPPTEKIDTTSVAKFSGTTNALPSDADTAVKSERSNYFDAGLSWQATAALTL
                     GLDAYDREVRHLQDEGQFGNALVYSAFNYDRGRIRGVELSANLHQGGLTAWGNLALSR
                     AEGKGLETGQFNFDPDEIGYIDARWVHLDHDQLATASAGFSYRFPTKTTVGADALFGS
                     GLRNGFANTQHLPSYMQLNASVAQSLDLGRGLGPVELRLSLLNLLDKAYELRDGTGIG
                     VGAPQFGPRRAAYVSVTKSF"
BASE COUNT          377 a          976 c         1141 g          430 t
ORIGIN      
        1 tcagcagcgc cagcgccgcc gccggatcgg aggccgccag cgccgcgacc gacgcgatgg
       61 cggcctccgc cgcgtcgcgc tgagcccggc gggatccgaa aaggaatcag gccgcacggt
      121 ggcggcctga tcggcgatgc ccggccgcgc gaaggggcgg gcgtcgatgc ccgggaacgc
      181 acctcgggcg ggacgggggg cggctcactg gcgcgtcagc ggcacgtcgg cccacggacc
      241 gtcgcggccg ggggcgcgca cctcgggcat gcgcgcgggc ctgcggtgcg tcggtgcgcc
      301 cgggacgggc cagttgagat cctcgacgaa gacgaggcgc ggcgcgcgca cggacggcgg
      361 cgcgcaggag cggcggccgg tatggcttgc ggcaatggaa ttcatggcgg acatccggta
      421 tcgggccgtc cgcccgcgcg gcgtgcgggg agcggacggc tcggggtcac ggtcgatcag
      481 cggcgatcgc ccttctgctg ctggcggccc gactgctggt cgatgctgcc gctgccgctg
      541 cggctctggt cgtcatcgcg caggttgcgg cccgattgcg gcgcgttgct gtcgcgggtc
      601 gacgacgagc gggaggactg gtcctgttgc gagggcgact tgtcctgctt gctctggttc
      661 tgctggctca tgagaaatct ccagaagtga cgccggcggc cccatgtcgc cggtccagat
      721 ggcggagcgc gcgcgccgcc aaccgggatg cggcaatggg ggcgcccgcc ctccccggat
      781 tcgcataccc ggggaggtaa agacgccgca accttcagaa ggacttcgtg acgctcacgt
      841 atgccgcgcg gcgcggcccg aactgcggcg cgccgacgcc gatgccggtg ccgtcgcgca
      901 gctcgtacgc cttgtccagc aggttcagca gcgacagccg cagctcgacg ggcccgagcc
      961 ccctgccgag atccagcgac tgggccaccg acgcgttgag ctgcatatag ctcggcaggt
     1021 gctgcgtgtt ggcgaagccg ttgcgcaggc cggagccgaa cagcgcgtcc gcaccgacgg
     1081 tcgtcttcgt ggggaagcga taggagaagc cggccgaggc ggtggccagc tggtcgtggt
     1141 cgaggtgcac ccagcgagcg tcgatgtagc cgatctcgtc ggggtcgaag ttgaactgcc
     1201 ccgtctcgag ccccttgccc tcggcgcgcg acagcgcgag gttgccccag gccgtcagcc
     1261 cgccctggtg caggttggcg ctcagctcga cgccgcggat gcgcccgcgg tcgtaattga
     1321 acgcggagta gaccagcgcg ttgccgaact ggccctcgtc ctgcaggtgg cgcacctcgc
     1381 ggtcgtaggc gtccaggccc agcgtcagcg cggcggtcgc ctgccacgag aggccggcgt
     1441 cgaagtagtt cgagcgctcg gacttcaccg cggtgtcggc gtcggagggc agcgcattgg
     1501 tcgtgccgga gaacttcgcc acggaggtgg tgtcgatctt ctcggtcggc ggcggcgtga
     1561 agtagcgcgc gtagccggcg tgcacgcgca gcgtgggcgt cgcgtcccag gtcaggccga
     1621 ggcgcgggct gagctggtgc tcgcgcacga ccgtgtcgac gtcgtcgaag cgcgcgccgt
     1681 agttgatcgt cagccccttg gccggctgcc actcgtcctg caggtacagg ccccagagcc
     1741 ggccgtggat cgcggtgtcg tcgacgatgg tctccggcac gtcgctcgtc tggtggccgt
     1801 cggcgtcggc ggggaagacc gcggaggagt tgtcgacgac gaagcgctcg cgctgcgcga
     1861 agaacccgcc gcgcagcgta tgggctcggc cgagcgcgac gctgccgtcg aactgcaggc
     1921 cgtcggccgt gttgcggcgc aggatctcgg cggcgatgcc gtcgaagacg aggtcgccga
     1981 ccggatcggg cgtgtagcgg acgtcggtgg cgcggtggaa gtacgagagc tggtagtcga
     2041 cgccgccgtc ggcctggccg gcatggccct gccacgagag cacctcgaac tggttgcgct
     2101 cgcgctggcg ggcgtcgagc gcggcggagt cgatgccgga cgcgccggcg agcgagtacg
     2161 acggcgcctg gccgggcacg tccgggagct ggaagcggtt gttcgaggtg ccgaacatca
     2221 ggttgagccg gtcgtcgtcg tcgagcacgt acgacaccgt gccgaagccc ttggcctggc
     2281 gcgtcgtgtc gtgcagcgcg tccttcgagg cggtcgggtt ctcgatgccc aggccgtcgc
     2341 gcagcagcga gccggtcagg tagtagctga cggccccgtg cgtgccgccg atctcgccgc
     2401 tgccttcctc gtgggcattg ctgccgccgg tccagctgac gctgccggag tcgcccgcgg
     2461 gctgcgcctt ggtgcggatg tcgacgaccg cggccgtgcg gtagccgtac tgcgccggca
     2521 gcgcgccggt caggatggcg atgttgtcgg cgaagcgggt ctcgagcgac tgcccgaagc
     2581 cgctgatcgc ctcggggatg acgacgccgt cgatgcggta ctgcacgttg gcgtggtcgc
     2641 cgcgcacgtg cagctggccg tacgaatcct gcaccacgcc gggcgactgc agcacgacct
     2701 ggttgagcgg cgtcgcctcg cccagcggca gcgccgcgat gtcggcgtcg ctgaacttgt
     2761 actggctggt gccggtgtcg ggcgagagcc cgttgcgggc ggcgtccagc cgctggcgca
     2821 cgccgtcgac gacgacgttc tgcaccgcgg cgatggggcc ggcggacggc gcatcgacgg
     2881 ccgccctgcc ggcgacgctg gccgcggagg cgggcgccgc ggcc
//
LOCUS       DASVLN010000543         4461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_243004, whole genome shotgun sequence.
ACCESSION   DASVLN010000543 DASVLN010000000
VERSION     DASVLN010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243004"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1250)
                     /locus_tag="VF453_13125"
     CDS             complement(33..1250)
                     /locus_tag="VF453_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX7688647.1"
                     /translation="MSPVKILVAQGGGPTAVINQSLVGAVLEARRFPQVARVYGARHG
                     VRGIVNEDFLDLTQETRHNLEGVARTPASALGSTRDKPDLKYCREILAVLQAHDIGVF
                     LYIGGNDSSDTVRIVAEEARKAGHPLRCIHIPKTIDNDLVGNDHTPGFPSAARFVAQA
                     FAGANLDNAALPGVYVGVVMGRHAGFLTAAASLSRQLPDDGPHLVYVPERSFALPRFL
                     ADVKACHERLGRCVIAVSEGIHDESGATIAEKLAKNVERDAHGNVQLSGTGALADLLC
                     DEIKSRLGIKRVRGDTFGYLQRSFLGCVSEVDQHEAREVGERAVHFALWGDRDGSVAI
                     RRTGHYAVDYVLQPLEAVAAKTRTMEDVLLAEAGNDVSPAFRDYLAPLVGADLPAPHR
                     LRAPAVAKVLGKA"
     gene            complement(1288..2511)
                     /locus_tag="VF453_13130"
     CDS             complement(1288..2511)
                     /locus_tag="VF453_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3734 domain-containing protein"
                     /protein_id="HEX7688648.1"
                     /translation="MPHLPRPPFETIALVLQGGGALGAYQAGVVEGLDEAGIEPDWVA
                     GISIGAINAAIIAGNPPATRLARLKDFWTTVTQPALWPDAMPWLDAHVAPMNDWRREV
                     WNGLSAWRAITEGQRGFFLPRAPLSWLGTPAPRTASFYDTAPLKATLERLVDFDRVNH
                     GPMRMTISAVDVETGNFEVFDNQHGRWRHRLRAEHVMASGALPPGFPAIAIEEPGPVD
                     PARDTGKPRGLRWFWDGGLVSNTPLSQVLDGHPRQDTLAFQVDLWSARGKVPTNVIDA
                     QERVKDIQYSSRTRTITDTQAQEQRQRRLLRELLERIPPEVREFDPACRAAAQQATAR
                     CVSVIQLIYQEKEWDGLSKDYEFGAVTMRNHWDSGLADIRATLEHRHWLERPEGDDPF
                     VTHDIHRQRRLAREA"
     gene            2601..3944
                     /locus_tag="VF453_13135"
     CDS             2601..3944
                     /locus_tag="VF453_13135"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX7688649.1"
                     /translation="MTASPSALLASELAAAGIRDVQCVFPDVSGVPRGKLMPAAAFAA
                     GAELRIAQAIPMQCVTGEYSYDPIFPDQDPDVILVPDLATLRAVPWAATPRAMAIHDC
                     FELDGSPCRFAPRTILKDVLARYAARGLVPVVAPEIEFYLVAPVDDADAPLRAPAMRA
                     GRREVGQSAFGLDTLDEHAAFWAELRLALDTLGVRADTWIHEVGLTQYEINLVHGDAL
                     AAADQAFLFKMAAKEVALRHGMQAVFMAKPIAGQPGSSMHLHQSVVDAAGRNVFSADD
                     GSETAAFRHFLGGLQANLPELMLVFAPFVNSYRRFVKQSQAPVNFHWGVDNRTTGLRI
                     PRSGPAARRVENRVAGADANPHLAIAATLAAGLDGLERELAPTPELATSAYEEAHALA
                     RTFPAALDAFRGGAAAVRLFGERFCTAYAAVKELEYEHWLAEIGAWERRYLAAQA"
     gene            3947..>4461
                     /locus_tag="VF453_13140"
     CDS             3947..>4461
                     /locus_tag="VF453_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7688650.1"
                     /translation="MADTSPRTSPHVAVVGGGPAGLMAASAMQDEGARVEVFDAMPSV
                     GRKFLLAGIGGLNLTHSESLPAFVKRFGDRTATLAPLLEAFGPDAARAWAASLGIETF
                     VGTSGRVFPKDMKAAPLLRAWLHRLRAGGQGRPAVRFHQRHRWLGWSGDGALRFETPD
                     GETARAFDGVVL"
BASE COUNT          556 a         1606 c         1657 g          642 t
ORIGIN      
        1 tgtcctcgtc ggacggtggc gccgccatcg actcacgcct tgccgagcac cttggccacc
       61 gccggcgcgc gcaggcggtg cggcgcgggc aggtcggcgc ccaccagcgg cgcgaggtag
      121 tcgcggaacg ccgggctcac gtcgttgccg gcctcggcga gcaggacgtc ctccatcgtg
      181 cgcgtcttcg cggcgacggc ctccagcggc tgcagcacgt agtcgacggc gtagtggccg
      241 gtgcggcgga tcgccaccga gccgtcgcga tcgccccaca gcgcgaagtg cacggcccgc
      301 tcgccgacct cgcgcgcctc gtgctggtcg acctccgaga cgcagccgag gaacgagcgc
      361 tgcaggtagc cgaaggtgtc gccgcgcacg cgcttgatgc ccagccggga cttgatctcg
      421 tcgcagagca ggtcggccag cgcgcccgtg cccgagagct gcacgttgcc gtgggcgtcg
      481 cgctcgacgt tcttcgcgag cttctcggcg atggtcgcgc ccgactcgtc gtggatgcct
      541 tcggagaccg cgatcacgca gcgcccaagg cgctcgtggc aggccttcac gtcggcgagg
      601 aacctcggca gcgcgaagct gcgctcgggc acgtagacga ggtgggggcc gtcgtcgggc
      661 agctggcgcg agagcgaggc cgccgccgtg aggaagcccg cgtggcggcc catcaccacg
      721 ccgacgtaga cgcccggcag cgccgcgttg tcgaggttcg cgcccgcgaa cgcctgggcg
      781 acgaagcgcg ccgccgaggg gaagccgggc gtgtggtcgt tgcccacgag gtcgttgtcg
      841 atcgtcttcg ggatgtggat gcagcgcagc gggtggcccg ccttgcgcgc ctcctcggcg
      901 acgatgcgca ccgtgtcgga cgagtcgttg ccgccgatgt agaggaacac gccgatgtcg
      961 tgcgcctgca gcaccgcgag gatctcccgg cagtacttca ggtcgggctt gtcgcgcgtc
     1021 gagcccagcg ccgacgcggg cgtgcgcgcg acgccctcga ggttgtggcg cgtctcctgc
     1081 gtgaggtcga ggaagtcctc gttcacgatg ccccgcacgc cgtggcgggc gccgtagacg
     1141 cgcgcgacct gcgggaagcg gcgcgcctcg agcacggcgc cgacgagcga ctggttgatg
     1201 accgcggtcg ggccgccgcc ctgcgcgacg aggatcttca cgggactcat ggacgcgtct
     1261 ccttggtgcc ggtgcggccg cgcgatctta agcctcgcgg gccaggcggc gctgccggtg
     1321 gatgtcgtgc gtcacgaacg ggtcgtcgcc ctccggccgc tcgagccagt ggcgatgctc
     1381 cagcgtcgcg cggatgtcgg ccaggccgct gtcccagtgg ttgcgcatgg tgacggcgcc
     1441 gaactcgtag tccttggaca gcccgtccca ctccttttcc tggtagatca gctggatcac
     1501 cgacacgcag cgcgcggtgg cctgctgcgc ggcggcgcgg caggcggggt cgaactcgcg
     1561 cacctcgggc gggatgcgct cgagcagctc gcgcagcagc cggcgctggc gctgctcctg
     1621 cgcctgcgtg tcggtgatgg tgcgggtgcg gctcgagtac tggatgtcct tcacgcgctc
     1681 ctgggcgtcg atcacgttgg tcggcacctt gccgcgcgcg ctccagaggt cgacctggaa
     1741 cgccagggtg tcctggcgcg gatggccgtc caggacctgc gacagcggcg tgttcgagac
     1801 cagcccgccg tcccagaacc agcgcaggcc gcgcggcttg ccggtgtcgc gggcgggatc
     1861 gacggggccg ggttcctcga tggcgatcgc cgggaagccg ggcggcagcg cgcccgaggc
     1921 catgacgtgc tccgcgcgca ggcggtggcg ccagcggccg tgctggttgt cgaagacctc
     1981 gaagttgccg gtctcgacgt cgaccgccga gatcgtcatc cgcatcggcc cgtggttgac
     2041 gcggtcgaag tcgaccaggc gctccagcgt ggccttcagc ggcgcggtgt cgtagaagct
     2101 cgccgtccgc ggcgccggcg tgccgagcca ggagagcggg gcgcgcggca ggaagaagcc
     2161 gcgctggccc tcggtgatcg cgcgccaggc gctcaggccg ttccagacct cgcgccgcca
     2221 gtcgttcatg ggcgcgacat gggcgtcgag ccagggcatc gcgtcgggcc acagcgcggg
     2281 ctgcgtcacg gtcgtccaga agtccttcag gcgcgccagc cgcgtggcgg gcgggttgcc
     2341 ggcgatgatc gccgcgttga tggcgccgat ggagatgccg gcgacccagt ccggctcgat
     2401 cccggcctcg tccaggccct cgacgacgcc cgcctggtag gcgccgagcg cgccgccgcc
     2461 ctgcagcacg agggcgatcg tctcgaaggg cgggcggggc aggtgcggca tggcgggcgg
     2521 ttccgtcgtc gtggaggcgg ggcgaagctt atcgagtccg catgtcgcag cgctgaaccg
     2581 ggatttgctc taatcgccgg atgaccgcct ccccctccgc cctgctggcc tcggagctgg
     2641 ccgccgccgg catccgcgac gtccagtgcg tgttcccgga cgtctccggc gtccctcgcg
     2701 gcaagctcat gcccgcggcc gccttcgccg ccggcgccga gctgcgcatc gcccaggcga
     2761 tcccgatgca gtgcgtgacg ggggagtact cgtacgaccc gatcttcccc gaccaggatc
     2821 cggacgtgat cctcgtgccc gacctcgcga cgctgcgcgc ggtgccctgg gccgcgacgc
     2881 cgcgggcgat ggcgatccac gactgcttcg agctcgatgg ctcgccctgc cgcttcgcgc
     2941 cgcgcacgat cctgaaggac gtgctcgccc gctacgccgc gcgcggtctc gtgccggtgg
     3001 tcgcgccgga gatcgagttc tacctcgtcg cgcccgtcga cgacgccgac gcacccctgc
     3061 gcgcgcccgc gatgcgcgcg ggccggcgcg aggtcgggca gtcggccttc gggctggaca
     3121 cgctcgacga gcacgccgcg ttctgggccg agctgcgact cgcgctggac acgctgggcg
     3181 tgcgcgccga cacctggatc cacgaggtcg gcctgacgca gtacgagatc aacctcgtgc
     3241 acggcgacgc gctggcggcg gccgaccagg ccttcctgtt caagatggcg gccaaggagg
     3301 tggcgctgcg ccacggcatg caggccgtct tcatggccaa gccgatcgcg ggccagcccg
     3361 gcagctcgat gcacctgcac cagagcgtgg tcgacgccgc gggccgcaac gtcttcagcg
     3421 ccgacgatgg aagcgagacc gccgcgttcc gccacttcct cggcggcctg caggcgaacc
     3481 tgccggagct gatgctggtc ttcgcgccct tcgtcaattc ctaccgccgc ttcgtgaagc
     3541 agagccaggc gccggtcaac ttccactggg gcgtcgacaa ccgcacgacg ggcctgcgca
     3601 tcccgcgcag cgggccggcg gcgcgacggg tcgagaaccg ggtcgccggc gcggacgcca
     3661 acccccacct cgccatcgcc gcgacgctgg ccgccggcct ggacggcctg gagcgcgagc
     3721 tcgcgcccac gcccgagctc gccaccagcg cgtacgagga ggcgcacgcg ctggcgcgca
     3781 ccttcccagc ggcgctcgac gcgttccgcg gcggcgcggc cgcggtgcgg ctgttcggcg
     3841 agcgcttctg caccgcctat gccgcggtca aggagctcga gtacgagcac tggctcgccg
     3901 agatcggcgc gtgggagcgg cgctacctcg cggcgcaggc gtgacgatgg ccgatacctc
     3961 accccgtacc tcgccgcatg tggcggtggt gggcggcggt ccggccgggc tgatggccgc
     4021 ctcggcgatg caggacgaag gcgcgcgcgt cgaggtcttc gacgcgatgc cctcggtcgg
     4081 ccgcaagttc ctgctcgcgg gcatcggcgg gctgaacctg acgcattcgg agtcgctgcc
     4141 ggccttcgtg aagcgcttcg gcgatcgcac ggccacgctc gcgccgctgc tggaggcctt
     4201 cgggcctgac gcggcgcgcg cctgggccgc gagcctcggc atcgagacct tcgtcggcac
     4261 ctcgggccgg gtgttcccga aggacatgaa ggccgcaccg ctcctgcgcg cctggctgca
     4321 ccgcctgcgc gccggcggcc aagggcggcc cgcggtgcgc ttccaccagc gccatcgctg
     4381 gctcggctgg agcggcgacg gcgcgctgcg cttcgagacg cccgatggcg agaccgcgcg
     4441 cgcgttcgac ggcgtggtgc t
//
LOCUS       DASVLN010000544         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_137191, whole genome shotgun sequence.
ACCESSION   DASVLN010000544 DASVLN010000000
VERSION     DASVLN010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137191"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VF453_13145"
     CDS             <1..1332
                     /locus_tag="VF453_13145"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7688651.1"
                     /translation="GMPVVPAAPRGRCIDDEGNDPRSVPPSRTVPGNTSATQAPVTAD
                     SRAPRMNLLKMVQDAIRRSNSVGAARLLAEAAQDDIDETRAGYLPTINATGQVGGVDA
                     GAPRTTTTRGSQYQAGVNASGPIWDGGRISDLAGWRTHLAEAARLGEIGAQEQVALQT
                     VSLALDRSRYAVEAQVYDQYANKMACLVDALQDIVAADRGRTSELVQARKTQQQAELM
                     RDQTLSQKRVVEVRLRRFVGDDLPPTEGITSVMLDIPDLQDLITIAGRAPEIAAQAEQ
                     ADALDSYVKAVLDGRKPQIGWLIAATHTGGVAPSRSMSGGITVSVPLFDPAISHTADA
                     ARKRALAAHMQRDDALEERKYRMTEVHEQAEHSLDRARHVVDILHNSELVRNYTLQQW
                     QQLGKRSLFDVMGAEADHYTMRVQYVDALYDTQQSNALLWSLGLGLAVHLQ"
     gene            1533..2090
                     /locus_tag="VF453_13150"
     CDS             1533..2090
                     /locus_tag="VF453_13150"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HEX7688652.1"
                     /translation="MNKLKMAAIAATAVLAGGACAAPATEHGAKTLTPVVDPSNPSET
                     DWTISNASFLAHGGTESGKKGDRFDDVYTFTLSNPYNLVDLGANATINRTPAVSFSGW
                     TLYDVSTHTTIDSAASLISNGVEVDDLNLGAGTYTFELKGTFVQAKGTYTGYVSAQAV
                     PEPAEAALLLAGLGLVGLVARRRKA"
     gene            complement(2127..3179)
                     /locus_tag="VF453_13155"
     CDS             complement(2127..3179)
                     /locus_tag="VF453_13155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7688653.1"
                     /translation="MKVAIAGGGWYGCHIASALKSQGVEVKLFERNERLFSEASGNNQ
                     FRLHQGLHYARSAITRHQSRDGYHRFVERYPRLSQGVAQNIYLVPKGASLIDFETYFS
                     IMLASGISVERVSATVVPGLDPNRFEGAVRCDERVVLTDKAREHFGGRLNGSAHLGTF
                     VESVRESTGSVAVNGERFDYFVDATWGALAPASEAWYYEPTLLLYYRCRASAANGAAE
                     RFPAVTLVDGPLWSIYPIGRDHQFTLSSVTHTPLGRCATKAEAYAVLAGIDDELVQRK
                     RAVMEAEVMPYLPAFRDLFEYVGPQFAVKTKPVGMADNRACGVAFDGRKVAVQSGKID
                     NIFFATDRILGALLAD"
BASE COUNT          521 a         1169 c         1176 g          440 t
ORIGIN      
        1 ggcatgccgg tcgtgcccgc cgcgccgcgc ggccgctgca tcgacgacga gggcaacgac
       61 ccgcgcagcg tgccgcccag ccgcaccgtg cccggcaaca cctcggccac gcaggcgccc
      121 gtcaccgccg actcgcgcgc gccgcgcatg aacctgctga agatggtgca ggacgcgatc
      181 cgccgcagca actcggtcgg cgcggcgcgg ctgctcgccg aggccgcgca ggacgacatc
      241 gacgagacgc gcgcgggcta cctgcccacc atcaacgcca ccggccaggt cggcggcgtc
      301 gacgccggcg cgccgaggac caccaccacg cgcggcagcc agtaccaggc cggcgtcaac
      361 gcgagcggcc cgatctggga cggcggccgc atctccgacc tcgccggctg gcgcacccac
      421 ctcgccgagg ccgcgcgcct gggcgagatc ggcgcgcagg agcaggtcgc gctgcagacg
      481 gtctccctgg cgctcgatcg cagccgctac gccgtcgagg cccaggtcta cgaccagtac
      541 gccaacaaga tggcctgcct ggtcgacgcg ctgcaggaca tcgtcgccgc cgaccgcggc
      601 cgcacgagcg agctggtgca ggcgaggaag acgcagcagc aggccgagct gatgcgcgac
      661 cagacgctgt cgcagaagcg cgtcgtcgag gtgcgcctgc gccgcttcgt cggcgacgat
      721 ctgccgccga ccgagggcat cacctccgtc atgctggaca tcccggacct gcaggacctg
      781 atcacgatcg ccggccgcgc gcccgagatc gccgcgcagg ccgaacaggc cgatgcgttg
      841 gacagctacg tcaaggcggt gctcgatggc cgcaagccgc agatcggctg gctgatcgcg
      901 gccacgcaca ccggcggggt cgcgccgagc cgctcgatgt cgggcggcat cacggtctcg
      961 gtgccgctgt tcgaccctgc gatctcgcac accgccgacg cggcgcgcaa gcgcgcgctg
     1021 gccgcccaca tgcagcgcga cgacgcgctc gaggagcgca agtaccgcat gaccgaggtg
     1081 cacgagcagg ccgagcactc gctcgatcgc gcgcgccacg tcgtcgacat cctccacaac
     1141 agcgagctgg tgcgcaacta cacgctgcag cagtggcagc agctgggcaa gcgatcgctg
     1201 ttcgacgtca tgggcgccga ggccgaccac tacacgatgc gcgtgcagta cgtcgacgcg
     1261 ctgtacgaca cgcagcagtc caacgcgctg ctgtggtcgc tgggcctggg gctggccgtc
     1321 cacctgcagt gacggcggcg gcgcggctta tcggcacgcg gcgcggccgg ccaagcgtcc
     1381 gcgtcgtcgc gatggcgggc atttcgtgat gcattgcgca cgacgcctcc ccagaagggg
     1441 tgagtcgccg cgatccaggc ccgtcctgac gggtgtttga cagcttgggt tcctagcatt
     1501 cctcccagtc gattcaaatt gggggtaatg agatgaacaa gctgaagatg gccgcgatcg
     1561 cggcgacggc ggtgctcgcc ggcggcgcct gcgcggcacc cgccacggag cacggcgcca
     1621 agacgctgac gccggtggtc gatccgtcga atccgtccga gacggactgg acgatctcga
     1681 acgcgtcctt cctggcgcac ggcggcaccg agtccggcaa gaagggcgac cgcttcgacg
     1741 acgtctacac gttcacgctg tcgaacccgt acaacctcgt ggacctgggc gcgaacgcga
     1801 cgatcaaccg caccccggcc gtcagcttca gcggctggac gctctacgac gtcagcacgc
     1861 acacgacgat cgacagcgcc gcgtcgctga tctccaacgg cgtcgaggtc gacgacctga
     1921 acctgggtgc cggcacctac accttcgagc tgaagggcac gttcgtgcag gccaagggca
     1981 cctacaccgg ctacgtgagc gcgcaggcgg tgccggagcc ggccgaagcg gcgctgctgc
     2041 tggcgggcct cggcctggtg ggcctggtcg cgcgccgtcg caaggcctga acctggagga
     2101 gaaggcgcgg ccgctcctgc ggcgtctcag tccgccagca gcgcgcccag gatgcggtcg
     2161 gtggcgaaga agatgttgtc gatcttgccg gactgcaccg ccaccttgcg gccgtcgaag
     2221 gcgacgccgc acgcgcggtt gtcggccatg ccgacgggct tggtcttgac cgcgaactgc
     2281 gggccgacgt actcgaacag gtcgcggaag gccggcaggt agggcatcac ctcggcctcc
     2341 atcaccgcgc gcttgcgctg cacgagctcg tcgtcgatgc cggcgagcac cgcgtaggcc
     2401 tcggccttgg tcgcgcagcg gcccagcggc gtgtgcgtga cggacgacag cgtgaactgg
     2461 tggtcgcggc cgatcgggta gatcgaccac agcggcccgt cgaccagcgt gaccgccggg
     2521 aagcgttccg cggcgccgtt ggcggccgag gcgcggcagc ggtagtacag cagcagcgtc
     2581 ggttcgtagt accaggcctc gctggccggc gcgagcgcac cccaggtggc gtcgacgaag
     2641 tagtcgaagc gctcgccgtt gacggccacg ctgccggtcg attccctgac cgactcgacg
     2701 aaagtgccca ggtgcgccga gccgttgagg cggccgccga agtgctcgcg cgccttgtcg
     2761 gtcagcacca cgcgttcgtc gcagcgcacg gcgccctcga acctgttcgg gtcgaggccc
     2821 ggcacgacgg tcgcggagac gcgctccacg gagatgccgc tggccagcat gatcgagaag
     2881 taggtctcga agtcgatcag cgacgcgcct ttcggcacga ggtagatgtt ctgcgcgacg
     2941 ccctgggaca gccgcgggta gcgctcgacg aagcggtggt agccgtctcg cgactggtgg
     3001 cgcgtgatgg ccgagcgcgc gtagtgcagg ccctggtgca ggcggaactg gttgttgccc
     3061 gaggcctcgc tgaacaggcg ctcgttgcgc tcgaacagct tgacctccac gccctgggac
     3121 ttcagcgcgc tggcgatgtg gcagccgtac cagccgcctc ctgcgatggc gaccttcatg
     3181 ccgcgggttc ctcgatcagg gccggcgcga ggaagcgggc gacgcgcgcg ctccagccga
     3241 cgttgcgaac cggctcgcgc gcgggcggcg cgtcgagcag ggcctgcgcc ggcgcgacga
     3301 gcgcgg
//
LOCUS       DASVLN010000545         6142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_514225, whole genome shotgun sequence.
ACCESSION   DASVLN010000545 DASVLN010000000
VERSION     DASVLN010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514225"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1092)
                     /gene="gspK"
                     /locus_tag="VF453_13160"
     CDS             complement(115..1092)
                     /gene="gspK"
                     /locus_tag="VF453_13160"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEX7688654.1"
                     /translation="MTRRSLPRRARGAAILLALLIMTLVATLAAGMVWLQWRGIEVEA
                     ADRARGQGQWLLNAALDWGDLILRSQIRNGHVQDDLTQPWATPLAETKLSSFLSADGS
                     HAASEDSGPDAYLSGQITDAQSKYNLYNLVADTRNETVRFELLCAQINVAQSTCAAIA
                     SNFTMSWDGASNVTSAQDHAQAVLMPQTLADLAWYGVDAKTIQQLEPYVVLIPPPPGQ
                     PLQVTKINANTAAAPVLAVAASGVQAMANNLVQQRQQNPFKSTRDIENALGGLTQDVG
                     LLTFQSDYFEIYGQLRYEQHVIRERSLVWRQNAALVTVLHRVRLPPDAQ"
     gene            complement(1089..1736)
                     /locus_tag="VF453_13165"
     CDS             complement(1089..1736)
                     /locus_tag="VF453_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7688655.1"
                     /translation="MKRTPPPAGFTLVEALVALLIMAILAAMAFRGVDAIVRARDGAQ
                     AATNRTLKLATGISQFEYDVSQVVNTGAVANPVDFNGGTLRLTRQAPGGVEIVVWTVQ
                     GRQWQRWASAPVTHFGQLSDQWMRSQQLGGLDGMVPVLDNVDDFQVYLCNPNNASGSA
                     NAGCSWNNAQTTRTVPPDGIRLTLKLPEGDFMREVLLPHWIAYSSSPRVPSSGTQ"
     gene            complement(1733..2104)
                     /gene="gspI"
                     /locus_tag="VF453_13170"
     CDS             complement(1733..2104)
                     /gene="gspI"
                     /locus_tag="VF453_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEX7688656.1"
                     /translation="MIGRGAGAARGFTLIEVLVAVAIVAVALAAGSRAASSVTDTSQR
                     LSDVTLAQWCADNELTELRLTATFPNVGTTPFDCTELGRAFKGMLKTQPTPNPSFRRV
                     DTIVADDTGHTLLTISTVVSR"
     gene            complement(2101..2646)
                     /locus_tag="VF453_13175"
     CDS             complement(2101..2646)
                     /locus_tag="VF453_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7688657.1"
                     /translation="MCSATAPTGSPAVKATTRTSAPGSEARGRVAAGFTLLELMVVML
                     LVAVATGMVTLSMRDASQNKLDEEGARLSAVLESARAQSRIVGTDVRWQPSQDGSSFQ
                     FVGLPSVTAAELPTRWLDADTRAEIVGQPQVLLGPEPMLPPQRIVLRLGGHELTVGSD
                     GLAPFAVIVPGADDGNRVAAQ"
     gene            complement(2573..3004)
                     /gene="gspG"
                     /locus_tag="VF453_13180"
     CDS             complement(2573..3004)
                     /gene="gspG"
                     /locus_tag="VF453_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEX7688658.1"
                     /translation="MKNTLNASRRARGFTLLELMVVIVIIGVLAALIAPAVLSRIGQA
                     KSTAAKADIQSIMNALKMYKLDNGRYPSADQGLDALVNKPTSGSVGNWKQELEKLPQD
                     PWGHPYQYANPGTHGEIDVFSYGADGQPGGEGDDADVGSWE"
     gene            complement(3239..4366)
                     /gene="icd"
                     /locus_tag="VF453_13185"
                     /pseudo
     CDS             complement(3239..4366)
                     /gene="icd"
                     /locus_tag="VF453_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
     gene            4630..5574
                     /locus_tag="VF453_13190"
     CDS             4630..5574
                     /locus_tag="VF453_13190"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEX7688659.1"
                     /translation="MRIGIVVDSACDLPREYLLRNAITVLPITLRVGDETLVDDRYPA
                     TTRRFLDERLGARGHSAETEPLGPAQIRDLFLERLVIDYDCVFCLTITATRSPIHAHA
                     SQASFAILKDYRPIRRNAGVPGPFLMRVIDTGNLFAAQGVTAVEAVRLREAGRTPGQI
                     RERLEQVAHNTYGYMLPRDLYYLRARAQKKGDRSVGWIGAALGTALDIKPLLRGYRGE
                     TGPVAKMRGFQPGAESLFAYAARRVRAGLLTPTLCVSYGGDLAELDAFAGYAALKETC
                     AGQGVEVFESPMSMTGMVNVGTGAITLGFAAAEHAFAV"
     gene            complement(5762..>6142)
                     /locus_tag="VF453_13195"
     CDS             complement(5762..>6142)
                     /locus_tag="VF453_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688660.1"
                     /translation="TAPAPTPPPVTTPPPAAAPVSVTSVDLGTAVGPDQKVTSPTTTF
                     SSKDTIYAAVSTTGTAANAVLNAKWTYQDGQTVNESSQTIAPNGDAVTSFHISKPDGW
                     PAGNYKVEISLDGTSAASKDFTVK"
BASE COUNT          857 a         2069 c         2218 g          998 t
ORIGIN      
        1 gggcggcccg cgccacgcaa aacggcgtat tgtggcctgc gccggcgacg gttccggcga
       61 ccgtcggtaa cacctcgggg cgggaaaacg ccgagaccgc ggattgcccg cgcgtcattg
      121 ggcgtccggc ggcagccgca cgcggtgcag gacggtcacg agcgccgcgt tctggcgcca
      181 gaccagcgag cgctcgcgga tcacgtgctg ctcgtagcgc agctggccgt agatctcgaa
      241 gtagtcggac tggaaggtca gcagcccgac gtcctgcgtg aggccgccga gggcgttctc
      301 gatgtcgcgc gtgctcttga aaggattctg ctggcgctgc tgcacgaggt tgttggccat
      361 cgcctgcacg ccggaggccg cgaccgccag caccggcgcc gcggcggtgt tggcgttgat
      421 cttcgtgacc tgcagcggct ggccgggcgg cggcgggatg agaacgacgt agggctcgag
      481 ctgctggatc gtcttggcgt cgacgccgta ccacgcgagg tcggccagcg tctgcggcat
      541 cagcacggcc tgcgcgtggt cctgcgcgga cgtgacgttg gaagcaccgt cccagctcat
      601 ggtgaagttc gacgcgatgg ccgcgcacgt cgattgcgcg acgttgatct gcgcgcacag
      661 cagctcgaag cgcaccgtct cgttgcgggt atcggcgacc aggttgtaga ggttgtactt
      721 cgactgcgcg tcggtgatct ggcccgagag gtaggcgtcc gggccgctgt cctcggacgc
      781 ggcgtggctg ccgtcggccg acaggaagct ggagagcttg gtctcggcca gcggcgtcgc
      841 ccagggctgg gtgaggtcgt cctggacgtg gccgttgcgg atctgcgagc gcaggatcag
      901 gtcgccccag tcgagcgcgg cgttcagcag ccactgtccc tggccgcgcg cgcggtcggc
      961 cgcctcgacc tcgatgccgc gccactggag ccacaccatg ccggcggcca gcgtggcgac
     1021 gagcgtcatg atgagtagcg cgagcaggat ggcggcgccg cgcgcgcggc gggggagcga
     1081 tcggcgcgtc attgggtgcc gctcgaggga acccgcgggg acgaggaata cgcgatccag
     1141 tgcggcagca gcacctcgcg catgaagtcg ccctcgggga gcttgagcgt caggcggatg
     1201 ccgtccggcg gcacggtgcg cgtcgtctgc gcgttgttcc agctgcagcc cgcgttggcc
     1261 gagccgctgg cgttgttcgg gttgcagaga tagacctgga aatcgtcgac gttgtcgagc
     1321 acgggcacca tgccgtcgag gccgcccagt tgctggctgc gcatccactg gtcgctcagc
     1381 tggccgaagt gggtgaccgg cgcgcttgcc cagcgctgcc actggcggcc ctgcaccgtc
     1441 cagacgacga tctcgacgcc gcccggcgcc tggcgcgtca ggcgcagcgt gccgccgttg
     1501 aaatccaccg ggttggccac cgcgcccgtg ttgacgacct gcgagacgtc gtactcgaac
     1561 tgcgagatgc ccgtggcgag cttgagcgtg cggttcgtcg cggcctgggc gccgtcgcgg
     1621 gcgcggacga tggcgtcgac gccgcgaaag gccatcgcgg cgaggatcgc catgatcagc
     1681 agcgcgacca gcgcctcgac gagggtgaag ccggcgggcg gcggggtgcg cttcatcgcg
     1741 acacgaccgt ggagatcgtc agcagggtgt ggcccgtgtc gtcggcgacg atcgtgtcga
     1801 cgcggcggaa gctcgggttg ggcgtcggct gcgtcttgag catgcccttg aacgcacggc
     1861 cgagctcggt gcagtcgaac ggcgtcgtgc cgacgttggg gaaggtggcg gtcaggcgca
     1921 gttcggtgag ctcgttgtcc gcgcaccatt gcgcgagggt gacgtcggac agccgctgcg
     1981 aggtgtcggt gaccgagctc gccgcccgcg agccggccgc gagggcgacc gcgacgatcg
     2041 cgaccgcgac cagcacctcg atcagcgtga agccccgggc cgcgcccgcg ccgcggccga
     2101 tcattgcgcc gcgacccggt tgccgtcgtc ggcgcccggc acgatcaccg cgaacggcgc
     2161 gaggccatcg ctgccgaccg tgagctcgtg gccgcccagg cgcaggacga tgcgctgcgg
     2221 cggcagcatg ggttccgggc cgagcagcac ctgcggctgg ccgacgatct cggcgcgcgt
     2281 atcggcgtcg agccagcgcg tgggcagctc ggccgcggtc acgctgggca ggccgacgaa
     2341 ctggaagctc gatccgtcct ggctgggctg ccagcgcacg tcggtgccga cgatgcgcga
     2401 ctgtgcacgg gcgctttcca gcaccgcgga caggcgcgcc ccttcctcgt cgagcttgtt
     2461 ctgcgaggcg tcgcgcatcg acagcgtcac catgcccgtg gccaccgcca ccagcagcat
     2521 cacgaccatc agctcgagca gggtgaagcc ggccgcgacg cggccgcgcg cctcactccc
     2581 aggagccgac gtccgcgtcg tcgccttcac cgccgggctg cccgtcggcg ccgtagctga
     2641 acacatcgat ctctccgtgg gtgccggggt tggcgtactg gtacggatgg ccccagggat
     2701 cctgcggcag cttctcgagc tcctgcttcc agttgccgac gctgcccgac gtgggcttgt
     2761 tcacgagcgc gtccaggccc tggtcggcgg acgggtagcg gccgttgtcc agcttgtaca
     2821 tcttgagcgc gttcatgatg ctctggatgt cggccttggc ggccgtggac ttggcctggc
     2881 cgatccggct cagcaccgcg ggcgcgatga gcgccgccag gacgccgatg atgacgatca
     2941 ccaccatcag ctcgagcagc gtgaagccgc gggcgcggcg cgatgcgttc agcgtgtttt
     3001 tcatgagggg ttccggaggg ggcggcgggc accaggtctt gaatcataat cgccgcatga
     3061 gttcccgctg gactggcttc ctcgtctggg ccctggtggc ggcatcgacc gcgttctggg
     3121 gcctgaagat cttcgccgcg acgcgcccgc tgcccgccag cgtgcagacg cccggcgacg
     3181 cgacgccccg gtccggcccg atggagcacg tgttcggcgc ggtcgtcgtg cccacggcgg
     3241 cggcctcggt ccagcccatg tagcgcagca tcatctcgcc ggacaggatc agcgaacccg
     3301 ggttgacctt gtcgaggccg gcgtatttcg gcgcggtgcc gtgggtcgcc tcgaacaccg
     3361 catggccggt cacgtagttg atgttgccgc cgggcgcgat gccgatgccg ccgacctgcg
     3421 cggcgagcgc atccgagaga tagtcgccgt tgaggttcag ggtcgcgacc acgtcgtatt
     3481 cggccgggcg cagcaggatc tgctgcagga acgcatcggc gatcgcgtcc ttgaccgtga
     3541 tcgtcctgcc ggtcttcgga ttcccgaagg tcatccatgg gccgccgtcg agttcgaccg
     3601 caccgaactc cttctgcgcg agggcgtagc cccagtcgcg gaacgcgccc tcggtgaatt
     3661 tcatgatgtt gcccttgtgc accagggtca ccgatgaacg gtcgttgtcg accgcgtatt
     3721 ccagtgccgc gcgcacgagg cgctcggtgc catccctcga gaccggcttg atgccgaagc
     3781 cggacgtgtc cgggaagcgc actttcttgt agcggtccgg gaagttgtcc ttcagcaacg
     3841 acgcgaactt cttcgcgtcg tcggtacctt cctggaattc gatgccggcg tagatgtcct
     3901 cggtgttctc gcggaagatc gtcatgtcga ccctggacgg atccttcacc ggcgaaggca
     3961 cgcccttgaa ccagcgcacc gggcgcaggc aggtgtagag gtcgagcatc tggcgcagcg
     4021 cgacgttcag cgagcggatg ccgccgccga ccggcgtggt cagcggaccc ttgatcgaga
     4081 cgaggtattc gcggcaggcg gcgacggttt cgtcgggcag ccagttgccg acccggttga
     4141 acgccttctc gccggcgagc acttccatcc agtggatccg gcgcctgccg ccgtaggcct
     4201 tggcgacggc ggcatccagc acgcgcaccg aggcgcgcca gatgtccggc cccgtgccgt
     4261 cgccctcgat gtacggcacg accggattgt cgggcaccgc gagccggccg tgcgcgatcg
     4321 agatcttggc gccgcctgcg ggcggcgtgg gtgtggcgtc cgtcattggg ttctccggat
     4381 gaggcgggag ggtgcgaccc cggcatgcgc ggggacagtc gcccattgtc gcgcgtccgg
     4441 cccgatcaat gaagcgcgtg ccggcgagcg gcggccggcg gccggcggac gcgcgaccgg
     4501 agcatgcagg ccgcgctccc gctggctggt cggcgtgcta ccgttgtgcc gccgccggca
     4561 tgcttcgcgg tggcacgatt cgggtgcggg cgaccgcagg ccgtcccgcg tgacaggagc
     4621 ggagcccgca tgcgcatcgg catcgtggtc gactcggcct gcgaccttcc gcgcgaatac
     4681 ctgctgcgca acgcgatcac ggtgctgccg atcaccctgc gtgtcggcga cgaaacactc
     4741 gtcgacgacc gctatccggc gaccacgcgg cgtttcctcg atgagcgcct cggtgcgcga
     4801 ggccattccg ccgaaaccga gccgctcggt cccgcgcaga tccgcgacct gttcctcgaa
     4861 cgactcgtga tcgactacga ctgcgtgttc tgcctgacca tcaccgcgac gcgcagtccg
     4921 atccatgccc acgcgagcca ggccagcttc gcgatcctga aggactaccg gccgatccgc
     4981 cgcaacgccg gcgtgcccgg cccgttcctg atgcgcgtga tcgataccgg caacctgttc
     5041 gccgcccagg gcgtgaccgc ggtcgaggcg gtgcgcctgc gcgaggccgg acgtactccg
     5101 ggccagatcc gcgagcgcct ggagcaggtc gcgcacaaca cctatggcta catgctgccg
     5161 cgcgatctct actacctgcg tgcgcgcgca cagaagaaag gcgatcgcag cgtcggctgg
     5221 atcggcgcgg cgctcggcac cgcgctcgac atcaagccgt tgctgcgcgg ttatcgtggc
     5281 gagaccggtc cggtggccaa gatgcgcggt ttccagccgg gcgccgaatc actgttcgcc
     5341 tatgcggcca ggcgcgtgcg cgccggcctg ctgaccccga cgctgtgcgt cagctatggc
     5401 ggcgacctcg ccgagctcga cgcgtttgcg ggctatgcgg cattgaagga aacctgcgcc
     5461 ggccaggggg tcgaagtgtt cgagagcccg atgagcatga ccggcatggt caatgtcggc
     5521 accggcgcga tcacgctcgg cttcgccgcc gcggaacacg cgttcgcggt ctgaagtcgc
     5581 gcggacatcg cccgcggcca gacgccccgc aggcgacgat ccgccggcgg cgtgcggcgc
     5641 ttgaccggca tccggccccg acacgaacgc gcggatcgct ccgcgccttc gtcgccggaa
     5701 ggacaaccgg ccgccgtgtc atcggccatc ccggcctgca cgggatgagt tcagcctgaa
     5761 ctcacttgac ggtgaagtcc ttgctcgcgg ccgaggtgcc atccagcgag atttcgacct
     5821 tgtagttgcc ggccggccag ccgtccggct tgctgatgtg gaagctcgtc accgcgtcgc
     5881 cgttcggcgc gatggtctgg ctcgactcgt tgacggtctg gccgtcctgg taggtccatt
     5941 tcgcattcag cacggcgttc gccgcggtgc cggtcgtcga cacggctgcg tagatcgtgt
     6001 ccttcgacga gaacgtcgtg gtcggcgagg tgaccttctg gtccggaccg acggcggtgc
     6061 cgagatcgac ggaggtgacc gacaccggcg ccgcggcagg cggcggcgtc gtgacgggcg
     6121 gcggtgtcgg cgccggtgcc gt
//
LOCUS       DASVLN010000546         2352 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_137285, whole genome shotgun sequence.
ACCESSION   DASVLN010000546 DASVLN010000000
VERSION     DASVLN010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137285"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VF453_13200"
     CDS             <1..599
                     /locus_tag="VF453_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7688661.1"
                     /translation="ALVPAVAQAFGRLDALVNSASRFEYDAPASFDPALLESLVRSNT
                     AAPVVLAQALAAQVARQPAAGGRITPCVVNLLDQKLANPNPDYFSYTLTKAALQHATV
                     LLAQALAPAVRVCGVSPGITLVSGPMDEAEFAAARKLTALGRSSTPEDIARAVRFLLE
                     SPAITGVDLAVDGGQHLCGQRRDVLFIAREQQAGEPVR"
     gene            637..1038
                     /locus_tag="VF453_13205"
     CDS             637..1038
                     /locus_tag="VF453_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX7688662.1"
                     /translation="MHSLLNHPSLMDCRRLFLRDYEVMINIGVHEFEKKGEQRVLINV
                     DLYVPLALSTPHADQLSEVLDYDFIRSTIAERMARGHVHLQETLCDDVLQLMLAHPQV
                     RAARVSTEKPDVYPDCASVGVEVFGIKETAQ"
     gene            1035..1979
                     /gene="ttcA"
                     /locus_tag="VF453_13210"
     CDS             1035..1979
                     /gene="ttcA"
                     /locus_tag="VF453_13210"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX7688663.1"
                     /translation="MSLEQRPDLPPTDAAAPDDAARRAAEKKQAFEINKLSKRLHRQV
                     GQAIIDFNMIEPGDKVMCCLSGGKDSYAMLDILLSLRQRAPVDFDIIAVNLDQKQPGF
                     PEHVLPDYLRARGVPFHIEEQDTYSIVTKHIEPGKTMCSLCSRLRRGILYRVAGELGA
                     TKIALGHHRDDIVVTMFMNMFFGSRLKGMPPKLVSDDGRNVVIRPLAYVNEDDLVRWA
                     AHREFPIIPCTLCGSQDNLQRVQIKAMIREWEKQYPGRIDNIFTAMGNITPSHMMDRA
                     LFPFTTIKASGRPEPEGDIAFDEDESCATPKPGVVRIV"
     gene            2143..>2352
                     /locus_tag="VF453_13215"
     CDS             2143..>2352
                     /locus_tag="VF453_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688664.1"
                     /translation="MSIVRSIPLALAAVLLGGCASLHTVTSTVASFGDWPAGSPPGTY
                     AFDRLPSQQASPQRQEAVEAAAAQAL"
BASE COUNT          381 a          887 c          764 g          320 t
ORIGIN      
        1 ccgcgctcgt ccccgcggtc gcgcaggcgt tcggccggct cgacgcgctc gtcaacagcg
       61 cctcgcgctt cgagtacgac gccccggcga gcttcgatcc cgcactgctg gagtcgctgg
      121 tgcgcagcaa caccgccgcg ccggtggtgc tcgcccaggc gctggcggcg caggtcgcgc
      181 gccagccggc ggccggcggc cggatcacgc cctgcgtcgt gaacctgctc gaccagaagc
      241 tcgccaaccc gaatcccgac tacttctcgt acacgctcac caaggccgcg ctgcagcacg
      301 cgaccgtgct gctcgctcag gcgctcgcgc cggcggtgcg cgtgtgcggg gtgtcgccgg
      361 gcatcacgct cgtctcgggg ccgatggacg aggccgaatt cgccgccgcg cgcaagctca
      421 cggcgctcgg ccgctcgtcc acccccgagg acatcgcgcg cgccgtgcgc ttcctgctcg
      481 agtcgccggc catcaccggc gtcgatctcg ccgtcgacgg cggccagcac ctgtgcgggc
      541 agcgccgcga cgtgctgttc atcgcccggg agcagcaggc cggcgagccg gtccgctgaa
      601 ggccccacga atccccatcc gcccgaaaga tccccgatgc acagcctgtt gaaccatccg
      661 tccctcatgg actgccggcg cctcttcctg cgtgactacg aggtgatgat caacatcggc
      721 gtgcacgagt tcgagaagaa gggcgagcag cgcgtgctga tcaacgtcga cctgtacgtg
      781 ccgctcgcgc tgtcgacgcc gcacgccgac cagctgtccg aggtgctcga ctacgacttc
      841 atccgcagca ccatcgccga gcgcatggcc cggggccacg tccacctgca ggagaccctg
      901 tgcgacgacg tgctccagct gatgctcgcg catccccagg tccgcgccgc gcgcgtgtcg
      961 accgagaagc ccgacgtcta ccccgactgc gcgtcggtgg gcgtcgaggt cttcggcatc
     1021 aaggaaaccg cccaatgagc ctggaacaac gacccgacct cccgccgacc gacgccgcgg
     1081 cacccgacga cgccgcccgc cgcgccgccg agaagaagca ggcgttcgag atcaacaagc
     1141 tctcgaagcg cctgcaccgc caggtcggcc aggcgatcat cgacttcaac atgatcgagc
     1201 ccggcgacaa ggtcatgtgc tgcctgtccg gcggcaagga cagctacgcg atgctcgaca
     1261 tcctgctgtc gctgcgccag cgcgcaccgg tggacttcga catcatcgcc gtcaacctcg
     1321 accagaagca gccgggcttc ccggagcacg tcctgcccga ctacctgcgc gcgcgcggcg
     1381 tgcccttcca catcgaggag caggacacct actcgatcgt cacgaagcac atcgagccgg
     1441 gcaagacgat gtgctcgctg tgctcgcggc tgcggcgcgg catcctgtac cgtgtcgccg
     1501 gcgagctcgg cgcgaccaag atcgcgctcg gccaccaccg cgacgacatc gtggtgacga
     1561 tgttcatgaa catgttcttt ggcagccgcc tgaaggggat gccgcccaag ctcgtgagcg
     1621 acgacggcag gaacgtcgtg atccggccgc tcgcctacgt caacgaggac gacctcgtgc
     1681 gctgggcggc gcaccgcgag ttcccgatca tcccctgcac gctgtgcgga agccaggaca
     1741 acctgcagcg cgtgcagatc aaggcgatga tccgggaatg ggagaagcag tacccgggcc
     1801 gcatcgacaa catcttcacc gcgatgggca acatcacgcc ctcgcacatg atggatcgcg
     1861 cgctgttccc gttcacgacg atcaaggcca gcgggcgccc ggagccggag ggcgacatcg
     1921 ccttcgacga ggacgagtcc tgcgccacgc ccaagccggg cgtcgtgcgc atcgtctgaa
     1981 acggcgcggg cgcgtcgcgg gatccacgtc cggggacagc tcgccgcaag ggcccgccgg
     2041 gcgcgccgcc tgccgggaca tactcgagat acacggccgc ccgacgatgg gcggacgcgc
     2101 cgcggcgcca tcggaccggc ctgcggcgac caggagaacg ccatgtccat cgtccgttcg
     2161 atcccgctcg cgctggccgc cgtcctgctc ggcggctgcg cgagcctgca caccgtcacc
     2221 agcaccgtcg ccagcttcgg cgactggccc gcgggcagcc cgcccggcac ctacgcgttc
     2281 gaccgcctgc cctcgcagca ggcgagcccg cagcgccagg aagccgtcga ggcggccgcg
     2341 gcgcaggcgc tg
//
LOCUS       DASVLN010000547         9335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_298590, whole genome shotgun sequence.
ACCESSION   DASVLN010000547 DASVLN010000000
VERSION     DASVLN010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298590"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VF453_13220"
     CDS             <1..1117
                     /locus_tag="VF453_13220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7688665.1"
                     /translation="KTPSPRACALAIAILLAGCTSPPAGHAPAADAAPPPSTYWRAAD
                     PATQGAACRAVLDDLHARVRAQPTTAMMAVRGDRVVFTEGPVAQASQIASACKSLLAM
                     LYGRPVADGRIHLDATLADLGIDDIGGLLPLERTARVRDLLAARSGVYHPAANGGDDA
                     DAAPPRGSRPPGTYFLYNNWDFNALGDIYEQRTGRDLYDAFADEIATPLGLEDWHRER
                     EHRSGDTSRSIHRAYYFHLSARDMARIGELVLADGRWDGRQLVPADWARTITAPVTHA
                     ADMHPARRARHAIGYGLLWWVPEPPAGSPLAPALAGSAMAWGYYGQWILVMPARGMVV
                     VHKFDTRARPDDAVQPRTLPVETFLREAVELADAPCA"
     gene            complement(1118..1957)
                     /locus_tag="VF453_13225"
     CDS             complement(1118..1957)
                     /locus_tag="VF453_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7688666.1"
                     /translation="MARRPRRSGRTLTAHAILAFFTLVALSPVALIVMNAFKSRRAIF
                     SSPLALPTPSTFDPVGFSTVLGEGHFQVWFANSVIVTVVSIAATLLFGAMAAFALSEY
                     KFRLNALTSLFLTIGLMVPIRLGTVGILEMMVAGGLVNTRTALILVYTAQSLPMAVFV
                     LDEFMRGLSGELKDAGRVDGLSEYRIFFGLVLPLLRPAMVSVAMFAMIPIWNDLWFPL
                     ILAPGEDTKTITLGAQVFIGQFVTDWNAVLAALSLSIVPVMALYLLFSRGLIRGITAG
                     AVK"
     gene            complement(1967..2941)
                     /locus_tag="VF453_13230"
     CDS             complement(1967..2941)
                     /locus_tag="VF453_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7688667.1"
                     /translation="MSEAIASSVAGAPARTRARRARRRPVRWHIGVFLAPALLVYVGV
                     MVLPLLETLRLAFLRRTDTGYVYDGVANFARLIGDPLWSHSFWNALANNTRFFLIHLL
                     VMVPIAIALAALLSLPRLRGSGFYRTALFMPTMLSYVIVGFVWKLILSPLWGITPRLM
                     RDVGLGAFFHPWLGRESSALTTLGLISVWQWLGIPMLLTYAAMLAIPDEILEASELDG
                     VTGLSQFFRIKLPLLWPTIGVVAILTFVNNFNSFDLIYTAQGALAGPDFSTDILGTLL
                     YRTFFGAQLQLGDPNMGAAIATLMLLIILGVVCAYLFLVQRRLSRYEL"
     gene            complement(2955..4229)
                     /locus_tag="VF453_13235"
     CDS             complement(2955..4229)
                     /locus_tag="VF453_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7688668.1"
                     /translation="MSMKRVSPNLPFAAVAAAISALAAAAAQAAPVTLSIESWRSEDL
                     AIWQNQIIPAFEKSHPNIQVKFTPTAPTEYNAALNSKLDAGTAGDLVACRPFDASLDL
                     FKKGKLASVNDLKGLANFTPVARAAWTTDDGKTTFCVPMASVIHGFIYNKDAFAKLKL
                     TPPQTVDQFFAVLDKIKADGEFVPLAMGTKDGWETASMGFQNIGPVYYKGEAGRKALI
                     AGKQKLTDPDWVAPWRVLARWKPYLADGFESQTYSDSQNMFTLGRAAIYPSGSWEISG
                     FNKQAQFKMGAFPPPVPKAGDKCYISDHPDIAMGVNAKSAHPAEARQFLEWVAGPEFA
                     ALYSNALPGFFSLNKNPVDVKDPLAREFVSWRAKCESTIRPTYQILSRGTPNLENESW
                     VVGSGVINGTITPEAAGQRLQAGLDKWYHPAK"
     gene            complement(4534..5469)
                     /locus_tag="VF453_13240"
     CDS             complement(4534..5469)
                     /locus_tag="VF453_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688669.1"
                     /translation="MNSPTTPPILPAGPEPNWEWYRSFLAVLDAGSLSAAARVLGLTQ
                     PTLGRHVDQLEQALALKLFTRSFDGFAPTEAALELRPYAAGLAANAAALRRVAASHGG
                     EVRGTVRITASEVIGVEVLPPLLAALRARHPALVFELVPSNEVGDLLRREADIAVRMV
                     RPTQGPLVARHVGGIECGLHAHRRYLEACGTPRTMDELAGHALVGFDRENAAIRRLQA
                     AYPALRRSDFALRADSDLAQLAAIRAGFGIGICQCALAARDPAIVRVLPSEFSVTLDT
                     WIAMHEDLRASPRCAVTFAALVDGLAAHAAGATAG"
     gene            5572..6540
                     /locus_tag="VF453_13245"
     CDS             5572..6540
                     /locus_tag="VF453_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7688670.1"
                     /translation="MTQAAPKIALFGAAGAIGNTVARSLAAQGTPYRVVGRHRAALER
                     DFGADPLAEIATWDPDSPASVRAAAEGIETIVYMVGVDYTRFELHPQLMRKTLDGAVA
                     AGVRRILLIGTVYPYGRPRTTPVREDHPREPHTFKGRMRKEQEDILLQAHADGRIGAA
                     VLRLPDFYGAGAGDKSLLAGVFKAAVEGGTADMIGPIDAPHEFVFVPDVGPVVARLIR
                     EPAAWGHVWHLAGAGTTSQKALLDEVSRQAGRPVRARVAGRTMLRVIGLFQPLLRELV
                     EMHYLQTQPVIMDDSALQRLIGPVAKTPYAEGVRRTLEALRTPAHA"
     gene            6675..7940
                     /locus_tag="VF453_13250"
     CDS             6675..7940
                     /locus_tag="VF453_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7688671.1"
                     /translation="MSAQPSTASAADAASTKPPPFSRYQKFVVGVLAFLQFAVIVDFM
                     LMAPLGVMIMPALSITPGRFGFAVSAYAFSAGLSSLVTAGFADRFDRKRLLLFFYGGF
                     VLGTLWCGLAGSYEALLGARIVTGLFGGVIGSVVIAIATDLFPPQMRGRVMGTIQTSF
                     AASQVLGIPVAVWLSNHWGWHVPFIAMAALGAAGGIVVAIKMRPVDGHLGKPQEHGAF
                     MHLWHTLTEPRYLLAFLTVTLLTTGGFMLMPFSSAYTVHNLGIDVTHLPLIYFVTGLC
                     TIFVGPLVGRMSDRVGRLKVFLFGSTLSVVMVLVYTHLPPVPIAALVVVNVVMFVGIF
                     SRMIPFQAMSASLPVPQQRGAYSAIGASIQQLAGAVASVVAGQIVVAEPDGRLLHFDV
                     VGYVMVATAAIAAWLVWRISIAMKTQPTH"
     gene            8371..8931
                     /locus_tag="VF453_13255"
     CDS             8371..8931
                     /locus_tag="VF453_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7688672.1"
                     /translation="MKKIALAALLAASFGAHAAVITFDGLNQNGTVPIANGYAGLNWS
                     DFYTTNAPVQFPGSGYAVDTISGEVAYNGYAQPATISATSSAGFTLNSGYFAAAWINE
                     NAVFTARFEDGSTATKTFAITTGSPVFETFGWHDLASVTMVGDGGSHIAVENLNAAAV
                     PEPAETSLLLAGLGAIALVARRRKQA"
     gene            complement(9002..>9335)
                     /locus_tag="VF453_13260"
     CDS             complement(9002..>9335)
                     /locus_tag="VF453_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688673.1"
                     /translation="LAPAQQGAFRDFVESVVEVGQHNERLLQKLLAQGVGSVSALDPV
                     GRFVEAEVRDGDERQQALQDLSERQQRLAALLDERQRSVLASLLADARGASAGKDGGP
                     GERPRPSP"
BASE COUNT         1399 a         3385 c         3221 g         1330 t
ORIGIN      
        1 gaagacgcct tccccacgcg cctgcgcgct cgcgatcgcc atcctgctcg cgggctgcac
       61 gtcgccacct gccggccacg cgccggccgc tgacgcggca ccgccgccct cgacgtactg
      121 gcgagcggcc gatcccgcga cgcaaggggc cgcctgccgc gccgtgctcg acgacctgca
      181 cgcccgcgta cgcgcgcagc cgaccaccgc gatgatggcc gtgcgcggcg accgtgtcgt
      241 cttcaccgag gggccggtcg cgcaggcctc gcagatcgcg tccgcgtgca agagcctgct
      301 cgcgatgctc tacggacgac ccgtcgccga cggccgcatc cacctcgacg cgacgctcgc
      361 ggacctcggc atcgacgaca tcggtggcct gctgccgctc gaacgcaccg cccgcgtgcg
      421 cgacctgctc gccgcgcgtt cgggcgtcta ccacccggcc gccaacggcg gcgacgacgc
      481 cgacgcggca ccgccacgcg gctcgcgccc gcccggcacc tacttcctct acaacaactg
      541 ggacttcaac gccctgggcg acatctatga gcagcggacc ggccgcgacc tctacgacgc
      601 cttcgccgac gagatcgcca cgccactggg cctggaggac tggcatcgcg aacgcgagca
      661 ccgcagcggc gatacgtcgc ggtcgatcca ccgcgcgtat tacttccatc tctccgcgcg
      721 cgacatggcg cgcatcggcg agctggtgct cgcggacggc cgctgggacg gccgccagct
      781 cgtgcccgcc gactgggcgc gcacgatcac ggcgcccgtc acgcacgcgg cggacatgca
      841 tccggcccgg cgcgcgcgcc acgcgatcgg ctacggcctg ctgtggtggg tgcccgagcc
      901 gccggccgga tcgccgctcg cgccggcgct ggccggctcg gccatggcct ggggctacta
      961 cgggcaatgg atcctcgtca tgccggcccg ggggatggtc gtcgtgcaca agttcgacac
     1021 ccgcgcacgg cccgacgatg cggtgcagcc gcgcacgctg ccggtcgaga ccttcctgcg
     1081 cgaggcggtc gagctcgccg acgcgccctg cgcctgatca cttcacggcg cccgccgtga
     1141 tgccgcggat caggccgcgc gagaacagca ggtacagcgc catcaccggc acgatgctca
     1201 gcgacagcgc ggccagcacc gcgttccagt cggtgacgaa ctggccgatg aagacctgcg
     1261 cgccgagcgt gatcgtcttc gtgtcctcgc ccggcgcgag gatcagcggg aaccacaggt
     1321 cgttccagat cgggatcatc gcgaacatgg cgacgctgac catcgccggc cgcagcagcg
     1381 gcagcacgag gccgaagaag atgcggtact ccgacagccc gtcgacgcgc ccggcgtcct
     1441 tcagctcgcc cgacagcccg cgcatgaatt cgtcgagcac gaagaccgcc atcggcagcg
     1501 actgcgccgt gtagacgagg atcagcgccg tgcgagtgtt cacgagcccg ccggcgacca
     1561 tcatctcgag gatgcccacg gtgcccaggc ggatcggcac catcaggccg atcgtcagga
     1621 acaggctcgt gagcgcattg aggcggaact tgtactccga cagcgcgaac gcggccatcg
     1681 cgccgaacag cagcgtcgcg gcgatggaca ccaccgtgac gatcacgctg ttggcgaacc
     1741 agacctggaa atgcccctcg cccagcacgg tcgagaagcc gaccgggtcg aaggtggacg
     1801 gcgtcggcag cgcgagcggc gacgagaaga tcgcgcgccg cgacttgaac gcgttcatca
     1861 cgatcagcgc gaccggcgac agcgcgacga gcgtgaagaa ggcgaggatc gcatgcgcgg
     1921 tcagcgtgcg tccggagcgg cggggtcggc gggccatggg gcgtcctcac aactcgtagc
     1981 gcgacaggcg ccgctgcacg aggaacaggt aggcgcacac gacgcccagg atgatcagca
     2041 gcatcagcgt cgcgatcgcc gcgcccatgt tggggtcgcc cagctgcagc tgcgcgccga
     2101 agaaggtgcg gtacagcaac gtgccgagga tgtcggtcga gaagtccggg ccggcgagcg
     2161 caccctgcgc cgtgtagatc aggtcgaagg agttgaagtt gttgacgaag gtcaggatcg
     2221 ccacgacgcc gatggtcggc cacagcagtg gcagcttgat gcggaagaac tgcgacaggc
     2281 cggtgacgcc gtcgagctcc gaggcctcga ggatctcgtc cgggatggcg agcatcgcgg
     2341 cgtaggtcag cagcatcggg atgcccagcc actgccagac cgagatcagc cccagcgtgg
     2401 tcagcgccga ggactcgcga cccagccacg ggtggaagaa cgcgcccagg ccgacgtcgc
     2461 gcatcaagcg cggggtgatg ccccacagcg gcgacaggat cagcttccag acgaagccca
     2521 cgatcacgta ggacagcatc gtcggcatga acagcgcggt gcggtagaag ccgctgccgc
     2581 gcagccgcgg caatgacagc agcgccgcca gcgcgatggc gatcggcacc atcaccagca
     2641 ggtggatcag gaagaaccgc gtgttgttgg cgagcgcgtt ccagaacgaa tgcgaccaca
     2701 gcggatcgcc gatcaggcgc gcgaaattgg cgacaccgtc gtacacgtac cccgtatccg
     2761 tacgacgcag gaacgcgagc cgcagcgtct ccagcagcgg cagcaccatc acgccgacgt
     2821 agaccagcag cgccggcgcg aggaacacgc cgatgtgcca gcgcaccgga cgccgccgcg
     2881 cgcgccgcgc ccgggtgcgc gcgggcgcgc cggcgacgga agaggcgatc gcctcgctca
     2941 tcgcggcctc gccttcactt ggcggggtga taccacttgt cgaggcccgc ctgcaggcgc
     3001 tgccccgcgg cctcgggcgt gatcgtcccg ttgatcacgc ccgagccgac gacccaggac
     3061 tcgttctcga ggttgggcgt tccgcgcgag aggatctggt aggtcggccg gatcgtcgac
     3121 tcgcacttcg cgcgccagct cacgaactcc ctggcgagcg gatccttgac gtccaccggg
     3181 ttcttgttga ggctgaagaa gccgggcagc gcgttcgagt acagcgccgc gaactcgggg
     3241 ccggcgaccc actcgaggaa ctggcgcgcc tcggccgggt gggccgactt cgcgttcacg
     3301 cccatcgcga tgtccgggtg gtcggagatg tagcacttgt cgccggcctt gggcaccggc
     3361 ggcgggaacg cgcccatctt gaactgcgcc tgcttgttga agccggagat ctcccacgag
     3421 cccgacggat agatcgccgc gcggccgagc gtgaacatgt tctggctgtc ggagtaggtc
     3481 tgcgactcga agccgtctgc caggtagggc ttccaccggg ccagcacgcg ccagggcgcg
     3541 acccagtcgg gatcggtcag cttctgcttg cccgcgatca gcgccttgcg gcccgcctcg
     3601 cccttgtagt agaccgggcc gatgttctgg aagcccatgc tcgcggtctc ccagccgtcc
     3661 ttggtgccca tcgcgagcgg cacgaactcg ccgtcggcct tgatcttgtc gagcacggcg
     3721 aagaactggt cgaccgtctg cgggggcgtc agcttgagct tggcgaacgc gtccttgttg
     3781 tagatgaagc cgtggatcac cgaggccatc ggcacgcaga acgtcgtctt gccgtcgtcg
     3841 gtcgtccacg cagcgcgcgc gacgggcgtg aagttggcca ggcccttgag gtcgttgacc
     3901 gaggcgagct tgcccttctt gaacaggtcg agcgaagcgt cgaacggccg gcaggcgacg
     3961 aggtcgcccg cggtgccggc gtcgagcttg gagttgaggg cggcgttgta ctcggtcggt
     4021 gcggtgggcg tgaacttcac ctggatgttc gggtgagact tctcgaacgc ggggatgatc
     4081 tggttctgcc agatcgccag gtcctcgctg cgccagctct cgatggacag ggtcacgggc
     4141 gccgcctgcg cggcggcggc ggccagggcc gagatcgcgg cggcgacggc ggcgaacggc
     4201 aggttcgggg acacgcgctt catgctcatg gtctgggact ttcttgtgtg gagggccgac
     4261 gcccggtgaa cgccggtccg ccgtcgcgcc gcgtgagcga tcgttcaccc acgcgtgccc
     4321 cggcagcccc ggccggggcc cactttgctg cgaaggcggc gcggtcgcat cgtggcaaac
     4381 cacgactcgc gtcgacgccc gcgcggaatg cgcgcgcgca ggccgaggat ccttcggcag
     4441 cgcgtgtccg gagcgcttcg gtccgaagaa aattcggaat ttctggaacc ggtaccaaac
     4501 ttgccggcac gaccgcttcg cccgcggcgg cggtcagccg gccgtcgcgc cggcggcgtg
     4561 ggcggccagg ccgtcgacca gcgcggcgaa ggtcaccgcg cagcgcgggc tcgcgcgcag
     4621 gtcctcgtgc atcgcgatcc aggtgtcgag ggtgaccgag aactccgacg gcagcacgcg
     4681 cacgatcgcc gggtcgcgcg cggccagcgc gcactggcag atgccgatgc cgaagccggc
     4741 gcggatggcg gccagctgcg cgaggtcgga gtcggcccgc agtgcgaagt ccgatcgccg
     4801 cagcgccgga tacgccgcct gcaggcgccg gatcgcggcg ttctcacggt cgaagccgac
     4861 cagcgcgtgg ccggcgagct cgtccatcgt gcggggcgtg ccgcaagcct cgaggtagcg
     4921 ccggtgcgcg tgcaggccgc actcgatgcc gccgacgtgg cgcgcgacca gcggcccctg
     4981 cgtcggccgc accatgcgca cggcgatgtc ggcctcgcgc cgcagcaggt cgccgacctc
     5041 gttggacggc accagctcga agaccagcgc cggatggcgc gcccgcagcg cggcgaggag
     5101 gggcggcagc acctcgacgc cgatcacctc gctcgcggtg atgcgcaccg tgccgcgcac
     5161 ctcgccgccg tggctggccg ccacccgccg cagcgccgcg gcgttggcgg ccaggccggc
     5221 cgcgtagggc cgcagttcga gcgcggcctc ggtgggcgcg aagccgtcga acgagcgcgt
     5281 gaacaacttg agcgccagcg cctgctcgag ctggtcgacg tggcggccga gcgtcggctg
     5341 cgtcagcccc agcacccgcg ccgcggccga cagcgagccg gcgtcgagca ccgcgaggaa
     5401 ggagcggtac cactcccagt tgggctcggg cccggccggc aggatggggg gtgtcgtcgg
     5461 gctattcatt ttcgtatgac cgcaagaaga ttctcggcga tttccatttg accaccgaca
     5521 tcccagactg gcgtcacccg attgaccaac gccccgccag gagacccgac catgacccaa
     5581 gccgccccca agatcgcgct gttcggcgcc gccggcgcca tcggcaacac cgtcgcccgc
     5641 agcctcgccg cgcagggcac gccctaccgc gtcgtcggcc gccaccgcgc ggcgctcgag
     5701 cgcgacttcg gcgccgaccc gctcgccgag atcgccacct gggatccgga ctcgcccgcc
     5761 tcggtgcgcg ccgcggccga gggcatcgag accatcgtct acatggtcgg cgtcgactac
     5821 acgcgtttcg agcttcaccc gcagctcatg cgcaagacgc tggacggtgc ggtcgccgcg
     5881 ggcgtccggc gcatcctgct gatcggcacg gtctatccgt acggccggcc gcgcacgacg
     5941 ccggtgcgcg aggaccaccc gcgcgagccg cacaccttca agggccgcat gcgcaaggag
     6001 caggaggaca tcctgctgca ggcccacgcc gacggccgca tcggcgccgc ggtgctgcgc
     6061 ctgcccgact tctacggcgc gggcgccggc gacaagagcc tgctcgccgg cgtcttcaag
     6121 gccgcggtcg agggcgggac cgccgacatg atcggcccga tcgacgcccc gcacgagttc
     6181 gtgttcgtgc ccgacgtcgg cccggtcgtc gcgcgcctga tccgcgagcc ggccgcatgg
     6241 ggccacgtct ggcacctcgc cggcgcgggc acgacctcgc agaaggcgct gctcgacgag
     6301 gtctcgcgcc aggccggccg ccccgtgcgc gcgcgcgtcg ccggcaggac gatgctgcgc
     6361 gtcatcggcc tcttccagcc cctgttgcgc gagctcgtcg agatgcacta cctccagacc
     6421 cagcccgtca tcatggacga ctccgccctc cagcgcctca tcggcccggt cgcgaagacg
     6481 ccctatgcgg agggcgtgcg gcgcacgctg gaggcgctgc gcacgccggc gcacgcctga
     6541 gcccggctcg cccgcgttcg accggcgtgg acggcgactc gtcgaccctc gccgcgcgtt
     6601 ggccgaaggc gcgcgcgcgc cgccggcgaa atgggcgagg atgcgccact tccaagaatc
     6661 ccgccaccac gcccatgtca gcccaaccgt ccaccgcgag cgccgccgac gcggcctcga
     6721 ccaagccgcc gccgttcagc cgctaccaga agttcgtcgt cggcgtcctc gccttcctgc
     6781 agttcgccgt catcgtcgac ttcatgctca tggcgccgct gggcgtgatg atcatgccgg
     6841 cgctgtcgat cacgccgggc aggttcggct tcgccgtctc tgcctacgcg ttcagcgccg
     6901 gcctgtcgag cctcgtgacc gccggcttcg ccgaccgatt cgaccgcaag cgcctgctgc
     6961 tgttcttcta cggcggcttc gtgctcggca cgctgtggtg cgggctggcc ggcagctacg
     7021 aggcgctgct cggcgcgcgc atcgtcacgg gcctgttcgg cggcgtgatc ggctccgtcg
     7081 tcatcgccat cgcgaccgac ctcttcccgc cgcagatgcg cggccgcgtg atgggcacga
     7141 tccagacctc gttcgccgcc agccaggtgc tgggcatccc ggtggcggtc tggctgtcga
     7201 accactgggg ctggcacgtg cccttcatcg ccatggcggc gctgggcgcg gccggcggga
     7261 tcgtcgtcgc gatcaagatg aggccggtcg acggccacct cggcaagccg caggagcatg
     7321 gcgcgttcat gcacctgtgg cacacgctga ccgagccgcg ctacctgctc gcgttcctga
     7381 ccgtcacgct gctgaccacg ggcggcttca tgctgatgcc gttcagcagc gcgtacacgg
     7441 tgcacaacct gggcatcgac gtcacgcacc tgccgctcat ctacttcgtc accggcctgt
     7501 gcacgatctt cgtcgggccg ctcgtgggcc gcatgtcgga ccgcgtcggc aggctgaagg
     7561 tgttcctgtt cggcagcacg ctgtcggtgg tgatggtgct ggtctacacg cacctgccgc
     7621 cggtgccgat cgcggcgctc gtcgtggtca acgtggtcat gttcgtgggc atcttctcgc
     7681 gcatgatccc gttccaggcg atgtccgcgt cgctgcccgt gccgcagcag cgcggcgcgt
     7741 acagcgcgat cggcgcgtcg atccagcaac tggcgggcgc ggtcgcgtcg gtcgtcgcgg
     7801 ggcagatcgt cgtcgccgag ccggacggcc ggctgctgca cttcgatgtc gtcggctacg
     7861 tgatggtcgc caccgccgcg atcgcggcct ggctcgtctg gcgcatcagc atcgcgatga
     7921 agacgcagcc gacgcactga cgcgtcgcgc gcgcgggcgc ggcgcgtccg ccacgagcca
     7981 aggtgccgct ggccgcccgc gagcgatgcg tcgctcgcgc cgccggcgat cgcctacgcc
     8041 tcggtccgca atgacaggcg catgccgggc gggcgccggc cgatggcttc gccgtcctgc
     8101 cgggcgacaa atcgcgccgc ctcgcgatcc gtgccctggc cgatccgggc tcatcgaccg
     8161 ggcgaggcgc cgcttaaatc cctcatcgtg aacaaagtcg cgaagcgatc gactgcttgg
     8221 gttggattcg atggtttcgg ctgtcgaatc gctgacaatt tgtaacgtcg cagtcgggcc
     8281 aacggatacc ggagggtcga cacccaaggg agccggggcc cctgccgcga aacagccccg
     8341 accaacgacc tgccatctgg aggagaagtc atgaagaaga tcgctctcgc cgcgctgctc
     8401 gcggcctcgt tcggcgccca tgccgccgtc atcacgttcg acggcctcaa ccagaacggt
     8461 acggtgccca tcgcgaacgg gtacgcaggc ctcaattgga gcgacttcta cacgaccaac
     8521 gccccggtgc agtttccggg cagcggctac gccgtggaca cgatctcggg cgaagtcgcc
     8581 tataacggct acgcgcagcc ggccaccatt tcggcgacct cgagcgccgg cttcacgctg
     8641 aactccggct acttcgccgc cgcctggatc aacgagaatg ccgtcttcac cgcccggttc
     8701 gaagacggct cgaccgccac caagaccttc gcaataacga ccggcagccc cgtattcgag
     8761 accttcggct ggcacgacct ggcctcggtc acgatggtcg gcgacggcgg ctcgcatatt
     8821 gcggtggaaa acctcaacgc tgcggccgtt ccggaacccg cggagacctc gctgctgctg
     8881 gccggcctcg gcgcgatcgc cctggtcgcc cgccgccgca agcaggcctg atccgcacgg
     8941 gcgcctggcg cccgccgcag gcccggacaa gccccgcccg ttcgccgcgc ggggcttttc
     9001 ttcatgggga cggacgcggc cgctcgccgg ggccgccgtc cttgcccgcg gacgcgccgc
     9061 gcgcgtccgc cagcaggctc gcgagcaccg accgctggcg ctcgtcgagc agtgcggcca
     9121 ggcgctgctg gcgctcgctc aggtcctgca gcgcctgctg gcgctcatcg ccgtcgcgca
     9181 cctccgcctc gacgaagcgg ccgaccgggt cgagggcgct cacgctgccg acgccctgcg
     9241 cgagcagctt ctgcagcagc cgctcgttgt gctggccgac ctcgacgacc gactccacga
     9301 agtcgcggaa cgctccctgc tgcgcgggcg cgagc
//
LOCUS       DASVLN010000548         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_243323, whole genome shotgun sequence.
ACCESSION   DASVLN010000548 DASVLN010000000
VERSION     DASVLN010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243323"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VF453_13265"
     CDS             <1..299
                     /locus_tag="VF453_13265"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HEX7688674.1"
                     /translation="VEAHAVDVAAELPRELNLLFIGSGASVAAAALVDAARARHALVV
                     TDLPDGLAVGAAIAFVQVDGRVRFEVSLAGARKCDLKLSSQLMGVAWRVLEDAP"
     gene            361..>1838
                     /locus_tag="VF453_13270"
     CDS             361..>1838
                     /locus_tag="VF453_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7688675.1"
                     /translation="MTIPTLRRRLTRASLLTTAAALVLDAALVIGYEFQRFRVDAIDN
                     LHTQQSILARAVAPSLVFNDPEAARQQLTSLAENPRVRMAEVFNAAGRPFARYVASAA
                     EAAQLPAANPLPRLGSGFVGDDVEIAYDIFHDGERIGTLYLRGHSGVARRLAVYALIQ
                     ALVMAASMGLALSVFGRVQRRITEPIIRVAQVAQEVVERRDWRLRAPSADTREVAVLV
                     DAFNRMLSEMGERTMQLRREIAERERAEAGLLAADRRKDEFLATLAHELRNPLAPMTN
                     AVALMRRVDCAPPVRDKALGILERQLRHVVRLIDDLLDVSRITTGKLSLLMEIVDLHA
                     VLRAAVELIDPTARDRQLHLDAAVPDEPCWLVGDAARLLQVFSNLLSNACRYTPPGGR
                     IRVTARCEGDVATAEVADTGVGIDPAMQERIFDLFEQGDKSLERGNAGLGLGLTLARQ
                     LVLLHGGTIGVNSRGIGHGSTFFVRLPRTARTPVVEPRPAAGG"
BASE COUNT          235 a          691 c          667 g          245 t
ORIGIN      
        1 cggtcgaggc gcacgccgtc gatgtcgcgg cggagctccc gcgcgagctg aacctgctgt
       61 tcatcggcag cggcgccagc gtcgccgcgg cggcgctcgt cgacgcggcc cgcgcgcgcc
      121 acgccctcgt ggtgaccgac ctgcccgatg gcctggccgt cggcgcggcg atcgcgttcg
      181 tccaggtcga cggccgcgtg cgcttcgagg tctcgctcgc cggcgcccgc aagtgcgacc
      241 tcaagctcag ctcgcagctg atgggggtcg cttggagagt gctggaggac gcgccatgag
      301 ccgccgcacg gccgctgcga agggggctcg caccgcagtc ccgagggcgg aggcttcccg
      361 atgaccatcc ccacgctgcg caggcggctg acgcgcgcgt cgctgctgac caccgccgcg
      421 gcgctcgtcc tcgacgcggc gctggtgatc ggctacgagt tccagcgctt ccgcgtcgac
      481 gcgatcgaca acctgcacac gcagcagagc atcctcgcgc gcgcggtcgc gccctcgctc
      541 gtgttcaacg accccgaggc agcgcgccag cagctcacct cgctggccga gaacccccgc
      601 gtgcgcatgg ccgaggtgtt caacgccgcc ggccgtccgt tcgcgcgcta cgtggcgagc
      661 gccgcggagg ccgcgcaact gcccgccgcc aatccgctgc cgcgcctggg cagcggcttc
      721 gtcggcgacg acgtcgagat cgcgtacgac atcttccacg acggcgagcg catcggcacg
      781 ctgtacctgc gcggccacag cggcgtggcg cggcggctgg cggtctacgc gctcatccag
      841 gcgctggtga tggcggcgtc gatggggctg gcgctgtccg tcttcgggcg cgtgcagcgg
      901 cgcatcaccg agcccatcat ccgcgtcgcg caggtcgccc aggaagtggt cgagcgccgc
      961 gactggcgcc tgcgcgcccc gtcggcggac acgcgcgagg tcgcggtgct ggtcgacgcg
     1021 ttcaaccgca tgctctccga gatgggcgag cgcacgatgc agctgcgccg cgagatcgcc
     1081 gagcgcgagc gcgccgaggc cggcctgctc gcggccgacc gccgcaagga cgaattcctc
     1141 gcgacgctcg cccacgagct gcgcaacccc ctcgcgccga tgacgaacgc ggtggcgctg
     1201 atgcggcgcg tggactgcgc gccgccggtg cgcgacaagg ccctgggcat cctcgagcgc
     1261 cagctgcgcc acgtcgtgcg cctcatcgac gacctgctgg acgtctcgcg catcacgacc
     1321 ggcaagctct cgctgctgat ggagatcgtc gacctccacg cggtgctgcg cgcggccgtc
     1381 gagctcatcg acccgacggc gcgcgaccgc cagctgcacc tcgacgccgc ggtgccggac
     1441 gagccgtgct ggctcgtcgg cgacgccgcg cgcctgctgc aggtgttctc gaacctgctg
     1501 agcaacgcct gtcgctacac gccgccgggc ggccggatcc gcgtgaccgc acgctgcgag
     1561 ggcgacgtcg cgaccgccga ggtggccgat acgggagtcg gtatcgaccc cgcgatgcag
     1621 gagcgcatct tcgacctctt cgagcagggc gacaagagcc tggagcgcgg caacgcgggc
     1681 ctggggctgg ggctcacgct ggcgcgccag ctggtgctgc tgcacggcgg cacgatcggc
     1741 gtgaacagcc gcggcatcgg ccacggctcg acgttcttcg tgcgcctgcc gcgcacggca
     1801 cggacgccgg tcgtcgagcc gcggcccgcg gccggcgg
//
LOCUS       DASVLN010000549         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_189899, whole genome shotgun sequence.
ACCESSION   DASVLN010000549 DASVLN010000000
VERSION     DASVLN010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189899"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..>2426
                     /locus_tag="VF453_13275"
     CDS             91..>2426
                     /locus_tag="VF453_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX7688676.1"
                     /translation="MAYSPPFQRPDAASPAASTVLKKPTKHQWIVAATTAGLAVAALV
                     VAVLTAAVVVNAQLPELDEITHYQPRQPLEILTRDGVELAQFGSERRRFMAIGDFPPL
                     LKRSLLATEDADFYEHSGISIKGIARAFLENLVHRHKQGASTITQQVARNFFLSRKQS
                     YSRKFAEALLAMKIERTLTKDQILELYMNQIYLGQHAYGFEAASLTYFGKPTKQLTPA
                     EVAMLAGLPKNPGGADPVSHFELAQKRQMVVLARMHETGVIDDAQYAQAKAQPLKIIG
                     KWTPPVSAEYLAEMVRQAVVQRFGEAAYSQGLRVTTSLVASDQEAAHEAVRRGVLDFD
                     RRQPYRGPEDNEDLPADDSDIAAAAAAALHDHPDDDDLHVAVVTHASPKEVVAILASG
                     ETVHLSGDGLRGAAAALKPKAPDDLAITRGAIIRVARTNGDASYKSEWRLAQWPDVEG
                     ALVSLDTATGQIRALVGGFDFSHSQFNHVTLAWRQPGSSFKPFIYSAALEHAVQPATL
                     INDAPIDIDPALTGGRHWEPKDDDNKFDGPMTARAALAQSKNLVSIRLVQMLGVNTVR
                     DWAHRFGFDPDKHPDNLTLALGAGSVTPMQMVRAYAAFANGGYGIVPRFIEKITDAQG
                     KVLFQAPPLPPAPTDDQRIIPARNAFVMASLLNEVTRTGTGAKAQATLKRSDVYGKTG
                     TTNDAVDTWFAGFQPKLATVVWMGRDDNTSLGPREFGATLALPIWIDYMRAALKGEPV
                     AVLPTPPGVQEQRGDWIYDEYAEGGYIAQLGMDPGNPPS"
BASE COUNT          397 a          916 c          809 g          304 t
ORIGIN      
        1 agcggggccg gtatcgccgc gcgatgccgg ccggcgcgcc gcgctgtccg gccgcgactg
       61 cggcgcgccc ggcggcgagg cgtcgcgtcg gtggcttaca gtccaccctt tcagcgtccc
      121 gacgccgcct cgcccgcggc cagcaccgtg ctcaagaagc cgaccaagca tcaatggatc
      181 gtcgcggcca cgaccgcagg actggccgtc gccgcgctcg tggtggcggt gctcaccgcg
      241 gcggtggtgg tcaacgccca gctgccggaa ctcgacgaga tcacccacta ccagccgcgc
      301 cagccgctcg agatcctcac gcgcgacggc gtcgagctcg cgcagttcgg ctcggaacgg
      361 cgccgcttca tggcgatcgg cgacttcccg ccgttgctca agcgctcgct gctggccacc
      421 gaggacgccg acttctacga gcacagcggc atcagcatca aggggatcgc gcgggccttc
      481 ctcgagaacc tggtccaccg ccacaagcag ggcgcctcga cgatcacgca gcaggtggcg
      541 cgcaacttct tcctgagccg caagcagtcc tactcgcgca agttcgccga ggcgctgctc
      601 gcgatgaaga tcgagcgcac gctgacgaag gaccagatcc tcgagctgta catgaaccag
      661 atctacctgg ggcagcacgc gtacggcttc gaggcggcct cgctcaccta cttcggcaag
      721 ccgacgaagc agctgacgcc ggccgaggtc gcgatgctgg ccggcctgcc caagaacccg
      781 ggcggcgccg atccggtctc gcacttcgag ctcgcccaga agcgccagat ggtggtgctc
      841 gcgcgcatgc acgagaccgg cgtcatcgac gacgcccagt acgcgcaggc caaggcgcag
      901 ccgctcaaga tcatcggcaa gtggacgcct ccggtgagcg ccgaatacct cgccgagatg
      961 gtgcgccagg ccgtggtgca gcgcttcggc gaggcggcgt actcgcaggg cctgcgcgtg
     1021 acgacctcgc tcgtcgcgtc cgaccaggag gccgcgcacg aggccgtgcg acgcggggtg
     1081 ctcgacttcg accgccgcca gccctaccgc ggccccgagg acaacgagga cctccccgcc
     1141 gacgactccg acatcgccgc cgccgcggcc gccgcgctgc acgaccatcc cgacgacgac
     1201 gacctgcacg tggcggtcgt cacgcacgcg agcccgaagg aagtcgtcgc catcctcgcg
     1261 agcggcgaga cggtgcacct gtccggcgac gggctgcgcg gcgcggccgc ggcgctcaag
     1321 cccaaggcgc ccgacgacct cgcgatcacg cgcggcgcca tcatccgcgt cgcgcgcacg
     1381 aacggcgacg cctcgtacaa gtccgagtgg cggctcgcgc agtggcccga cgtcgagggc
     1441 gcgctggtct cgctggacac ggccaccggc cagatccgcg cgctggtggg cggcttcgac
     1501 ttctcgcaca gccagttcaa ccacgtgacg ctcgcgtggc gccagcccgg gtccagcttc
     1561 aagcccttca tctactccgc cgcgctcgag cacgcggtgc agccggccac gctgatcaac
     1621 gacgcgccca tcgacatcga ccccgcgctg accggtggcc gccactggga gccgaaggac
     1681 gacgacaaca agttcgacgg gccgatgacc gcgcgcgcgg cgctcgccca gtcgaagaac
     1741 ctggtcagca tccgcctcgt gcagatgctg ggcgtcaaca ccgtgcgcga ctgggcgcac
     1801 cgcttcggct tcgacccgga caagcatccc gacaacctga cgctcgcgct cggcgccggc
     1861 agcgtcacgc cgatgcagat ggtgcgcgcc tatgcggcct tcgccaacgg cggctacggc
     1921 atcgtcccgc gcttcatcga gaagatcacc gacgcccagg gcaaggtgct gttccaggcg
     1981 ccgccgctgc cgcccgcgcc caccgacgac cagcgcatca tccccgcgcg caacgcgttc
     2041 gtgatggcga gcctgctcaa cgaggtcacg cgcaccggca ccggcgcgaa ggcgcaggcg
     2101 acgctcaagc gcagcgacgt ctacggcaag accggcacga ccaacgacgc cgtcgacacc
     2161 tggttcgccg gcttccagcc caagctcgcg accgtcgtgt ggatgggccg cgacgacaac
     2221 acgagcctcg gcccgcgcga gttcggcgcg acgctggcgc tgccgatctg gatcgactac
     2281 atgcgcgccg cgctcaaggg cgagccggtg gccgtgctgc cgaccccgcc gggcgtgcag
     2341 gagcagcgcg gcgactggat ctacgacgag tacgcggagg gcggctacat cgcgcagctg
     2401 ggcatggatc cgggcaaccc gccgtc
//
LOCUS       DASVLN010000550         3014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_243540, whole genome shotgun sequence.
ACCESSION   DASVLN010000550 DASVLN010000000
VERSION     DASVLN010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243540"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF453_13280"
     CDS             <1..220
                     /locus_tag="VF453_13280"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX7688677.1"
                     /translation="AHRAYLARGGLTTFLGDGSLRYGAERAAELYYDADLGAGLRLAA
                     DVQRVVHPGYDAARGPVSFYGLRLHWEV"
     gene            224..1531
                     /locus_tag="VF453_13285"
     CDS             224..1531
                     /locus_tag="VF453_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX7688678.1"
                     /translation="MNTHLRLPPRVNDQLNDFARRLGPGLITGAADDDPSGIATYSQI
                     GSQFRFGLAWTLLLTTPLMIGIQMLSARIGWVTGEGLAANIAKMCPRWLTFLLVTLLV
                     VANTINIAADIGAMGESVRLLAGGSATPYVIGFGLLSLVCQIVFSYERTVRLLKWLTL
                     GLFAYVAVILSVNVPWRQVFVEGAAPWLYFPRGVTASDYASMVVAVLGTTISPYLFFW
                     QASQEVEDEARRPEAVELRHHPAYAAEHLSRIKQDTAFGMVLSNVIALCIVVSTAVTL
                     NLHGITTIQTSSQAAQALRPVAGDFAFAIFALGIVGTGLLAVPVLAGSAAYAVSEAFG
                     WKSGLSHGFREARGFYLIIAAATVFGTLMSTLELDPIRALVWSAIVNGVISVPIMVVM
                     MWIGQSERLMGRFTISLRHRVFGWAATLVMAAAVAFMLVTSLR"
     gene            complement(1577..2671)
                     /locus_tag="VF453_13290"
     CDS             complement(1577..2671)
                     /locus_tag="VF453_13290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7688679.1"
                     /translation="MDPKVLKLQAHRLRASAMTLVSSLLQALLVGLFAWSGVVDDAAA
                     IAFAILSLGTTGAFTLAVSRGWNLRFRDSWLLHAQMLANFAIQVVFIVAAPRLWFLFL
                     ASTLVSFNYAMVGFSPVQFRWTWLGFGGATAAALWIGHDRFAWPALTPLNVALTWLFF
                     FLAMRRLGLVGMQFSALRAQLSERNKELVASVARIQALASHDELTGVFNRRHFLQLVG
                     DENERAARTGQPYSVALFDIDFFKSINDRHGHATGDQVLRAFCELVRQHLRATDRFAR
                     YGGEEFVLLMPATTTLENALPAVERIRLAIEAHDWATVSPRLAGHRVTTSAGVATSRA
                     GETVEELLARTDAALYRAKAQGRNRSVTAA"
     gene            complement(2821..>3014)
                     /locus_tag="VF453_13295"
     CDS             complement(2821..>3014)
                     /locus_tag="VF453_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688680.1"
                     /translation="LRRLLHALGHLLGTPGDLSAYERAKRRGGTVISVDVDGPPEAAL
                     AERAFVRAGAIDVTRRPHD"
BASE COUNT          417 a         1068 c         1084 g          445 t
ORIGIN      
        1 cgcgcaccgc gcctacctcg cccgcggcgg cctcacgacc ttcctcggcg acggctcgct
       61 gcgctacggc gccgagcgcg ccgccgagct gtactacgat gccgacctcg gcgcgggcct
      121 gcgcctggcc gccgacgtcc agcgcgtcgt gcacccgggc tacgatgccg cgcgcggccc
      181 ggtctccttc tacggcctgc gcctgcactg ggaggtctga cgcatgaaca cgcacctgcg
      241 cctgccgccg cgcgtcaacg accagctcaa cgacttcgcc cggcgcctcg gccccggcct
      301 catcaccggc gcggccgacg acgacccgag cggcatcgcg acctactcgc agatcggctc
      361 ccagttccgc ttcgggctcg cctggacgct gctgctgacg acgccgctga tgatcggcat
      421 ccagatgctg tcggcgcgca tcggctgggt caccggcgag ggcctggccg ccaacatcgc
      481 caagatgtgc ccgcgctggc tcaccttcct gctcgtgacg ctgctggtcg tggccaacac
      541 gatcaacatc gcggccgaca tcggcgcgat gggcgagagc gtgcgcctgc tcgccggcgg
      601 ctcggcgacg ccctacgtga tcggcttcgg gctgctgtcg ctggtgtgcc agatcgtgtt
      661 ctcgtacgag cgcacggtgc gcctgctcaa gtggctcacg ctgggcctgt tcgcctatgt
      721 cgcggtgatc ctgtcggtga acgtgccgtg gcgccaggtc ttcgtcgagg gcgcggcgcc
      781 gtggctgtac ttcccgcgcg gcgtgacggc gagcgactac gcgtccatgg tcgtggcggt
      841 gctgggcacg acgatcagcc cgtacctgtt cttctggcag gcgtcgcagg aggtcgagga
      901 cgaggcccgc cgccccgagg ccgtggagct gcgccatcac ccggcatacg cggcggagca
      961 cctctcgcgc atcaagcagg acacggcctt cggcatggtg ctgtccaacg tgatcgcgct
     1021 gtgcatcgtc gtctcgacgg cggtgacgct gaacctgcac ggcatcacca cgatccagac
     1081 ctcgtcgcag gcggcgcagg cgctgcggcc ggtggcggga gacttcgcgt tcgcgatctt
     1141 cgccctcggc atcgtcggca ccggcctgct cgccgtgccg gtgctcgcgg gctccgcggc
     1201 ctatgcggtc agcgaggcgt tcggctggaa gtccggcctg tcgcacggct tccgcgaggc
     1261 gcgtggcttc tacctcatca tcgccgcggc gacggtgttc ggcaccttga tgagcaccct
     1321 cgagctcgac ccgatccggg cgctcgtgtg gagcgcgatc gtcaacggcg tgatctcggt
     1381 gcccatcatg gtcgtcatga tgtggatcgg gcaatccgag cggctgatgg ggcgcttcac
     1441 gatcagtctg cgccaccgcg tcttcggctg ggcggcgacg ctcgtgatgg ccgcggccgt
     1501 ggccttcatg ctcgtgacca gcctgcgctg agcgtggggt cgcggcgtcg accgggcggg
     1561 acgctcgccc gcgacgctag gccgcggtga ccgagcggtt gcggccctgc gccttcgcgc
     1621 ggtagagggc cgcgtcggtg cgcgcgagca gctcctccac ggtctcgccc gcgcggctgg
     1681 tggcgacgcc ggccgaggtc gtcacgcgat ggcccgcgag gcgcggggag accgtggccc
     1741 agtcgtgcgc ctcgatggcg agacggatgc gctcgactgc cggcagcgcg ttctccagcg
     1801 tcgtcgtggc gggcatcagc agcacgaact cctcgccgcc gtagcgcgcg aagcggtcgg
     1861 tcgcgcgcag gtgctggcgc acgagctcgc agaacgcgcg cagcacctgg tcgccggtgg
     1921 cgtggccatg gcggtcgttg atggacttga agaagtcgat gtcgaacagc gccaccgagt
     1981 agggctggcc ggtgcgcgcg gcgcgctcgt tctcgtcgcc gaccagctgc aggaagtggc
     2041 ggcggttgaa cacgccggtg agctcgtcgt ggctcgcgag cgcctggatg cgcgcgacgg
     2101 atgcgacgag ctccttgttg cgctcggaca gctgcgcccg cagcgcgctg aactgcatgc
     2161 cgaccagccc cagccgccgc atcgcgagga agaagaacag ccaggtcagc gcgacgttca
     2221 gcggcgtgag cgcgggccag gcgaagcggt cgtggccgat ccacagcgcg gcggccgtcg
     2281 cgccgccgaa gcccagccac gtccagcgga actgcaccgg cgagaagccg accatcgcat
     2341 agttgaacga caccagcgtc gacgcgagga acaggaacca caggcgcggc gcggcgacga
     2401 tgaagacgac ctggatcgcg aagttggcca gcatctgcgc gtgcagcagc cacgaatcgc
     2461 ggaagcgcag gttccagccg cgcgagaccg cgagcgtgaa tgcaccggtc gtgcccaggc
     2521 tcaggatggc gaacgcgatc gcggcggcgt cgtcgaccac gcccgaccag gcgaacaatc
     2581 cgaccagcaa tgcctgcagc agcgagctga cgagcgtcat cgcgctcgcc cgcagtcggt
     2641 gggcttggag cttgagaacc ttgggatcca tgggagcggc ggccgcttca tgcgtcggcc
     2701 atcagggtgc cgcagcatac gggcagcccc gggcgggccg tcctcgcaca aacccacggc
     2761 gacgcgccgc cgccacggtg gcgggccgtg cgtgcctgga gccgcccggc gatcgccttc
     2821 tcagtcgtgc ggacggcgcg tgacgtcgat cgcgccggcg cggacgaacg cccgctcggc
     2881 cagcgcagcc tccggcgggc cgtcgacgtc gacgctgatc acggtgccgc cccggcgctt
     2941 ggcgcgctca taggcgctca ggtcgccggg cgtgcccaac aggtggccga gcgcgtgcag
     3001 caggcgacgc agcg
//
LOCUS       DASVLN010000551         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_29091, whole genome shotgun sequence.
ACCESSION   DASVLN010000551 DASVLN010000000
VERSION     DASVLN010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29091"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="VF453_13300"
     CDS             <1..111
                     /locus_tag="VF453_13300"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX7688681.1"
                     /translation="TAFAWLARQLVERRPGNHPAVTGAAGPRILGALHPA"
     gene            complement(178..549)
                     /gene="erpA"
                     /locus_tag="VF453_13305"
     CDS             complement(178..549)
                     /gene="erpA"
                     /locus_tag="VF453_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX7688682.1"
                     /translation="MTAVAELAPASTEMPAPIIFTDSAAAKVKELVDEEGNPELKLRV
                     FVQGGGCSGFQYGFTFDEAVAEDDTQMTKNGVTLLIDAMSLQYLTGAEIDYKEDLQGA
                     QFVIKNPNATSTCGCGSSFSA"
     gene            complement(642..1049)
                     /locus_tag="VF453_13310"
     CDS             complement(642..1049)
                     /locus_tag="VF453_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7688683.1"
                     /translation="MWFNRKKQPPIRSLIGEGTVVQGELRFDQGLRIDGEVVGDVVAS
                     ESQSLLVISENARVQGKVLAGHVIINGKVDGPVVATQLLELQPKARIVGDVHYEALEM
                     HQGATIDGELKHVTSADAEAERPLLKLAAANEG"
     gene            complement(1137..1856)
                     /locus_tag="VF453_13315"
     CDS             complement(1137..1856)
                     /locus_tag="VF453_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEX7688684.1"
                     /translation="MRWKLLRRRLSISAPRVTVRRHLAWPLRWLLAALVLGFSAATAL
                     WAFEVGKDLAGLDRDAKAELKRLRAEQDDLRAERDKADAVANTAQSLLTAERVTEERL
                     AAQLRQSEAEKLALQADLGFFQRLIPQGGGTPEGLVVRGFQAELQAPGHVRFQLLVMQ
                     GGRTPAQFNGFYEATLAGTRAGQPWTSPSSPATRQTLQVKQYARVEGMIDCPSDVVVK
                     TVQVKVTDAGGAVRVLQTLKL"
     gene            complement(1976..2368)
                     /gene="rpsI"
                     /locus_tag="VF453_13320"
     CDS             complement(1976..2368)
                     /gene="rpsI"
                     /locus_tag="VF453_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7688685.1"
                     /translation="MIGNWNYGTGRRKSSVARVFIKKGEGKIVVNGKTIEEYFGRQTS
                     IMIVKQPLMLTNNELAFDVKVNVHGGGESGQAGAVRHGITRALIDYDAALKGDLSRAG
                     FVTRDAREVERKKVGLHGARRRKQFSKR"
     gene            complement(2378..2806)
                     /gene="rplM"
                     /locus_tag="VF453_13325"
     CDS             complement(2378..2806)
                     /gene="rplM"
                     /locus_tag="VF453_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7688686.1"
                     /translation="MKTFSAKPAEVVHEWFVIDATDKVLGRVASEVAHRLRGKHKAIY
                     TPHVDTGDFIVIVNADKLRVTGNKAEDKVYYRHSGYPGGIYATKFKDMQAKHPGRALE
                     KAVKGMLPKGPLGYAMIKKLKVYAGATHPHAAQQPKALEI"
     gene            3184..3870
                     /locus_tag="VF453_13330"
     CDS             3184..3870
                     /locus_tag="VF453_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7688687.1"
                     /translation="MTAAPPEPTPDSASPARRPRAFGWVPIRSLSSRHRPRMLAHLLA
                     LGMGDRYLRFGYAAADDQIRRYVERIDFDRDEVFGVFNRRLELIALAHLAYEPEPELE
                     PGQAERPLAAEFGVSVALAARGRGYGARLFDHAVLRARNRGVDTIVIHALSENDAMIR
                     IVRNAGAHIEHTGGDSEAHLRLPPEPEAGAGQEADIEARLAELDYQFKQGARHMDTML
                     DIGAGTDPAR"
     gene            3896..>4148
                     /locus_tag="VF453_13335"
     CDS             3896..>4148
                     /locus_tag="VF453_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX7688688.1"
                     /translation="MTAAPDDRAPYARLTPHQMLDALAATGFPVTGGLLQLNSYENRV
                     VQAALEGGGAVVAKFYRPRRWSDSQILEEHDFARELAADD"
BASE COUNT          588 a         1487 c         1396 g          677 t
ORIGIN      
        1 accgcgttcg cctggctcgc gcgccagctc gtcgagcgcc ggccgggcaa ccaccccgcc
       61 gtcaccggcg ccgcgggccc gcgcatcctg ggcgcgttgc atccggcctg agcggctcgc
      121 gcgcgcccat gaaaaacggc gcccgcaggc gccgtcgtcc ggtcggcggg agtccgctca
      181 ggccgagaag ctcgagccgc agccgcaggt gctggtcgcg ttcgggttct tgatgacgaa
      241 ctgggcgccc tggaggtctt ccttgtagtc gatctcggcc cccgtgaggt actgcaggct
      301 catggcgtcg atcagcagcg tgacgccgtt cttggtcatc tgcgtgtcgt cctcggccac
      361 ggcctcgtcg aaggtgaagc cgtactggaa gccggagcag ccgccgccct gcacgaagac
      421 gcgcagcttc agctcgggat tgccttcctc gtcgaccagc tccttgacct tggccgcggc
      481 gctgtccgtg aagatgatgg gcgcgggcat ctcggtcgag gcgggggcca gttcggcgac
      541 ggcggtcatg tcggttcctt gcggttcggt tctgaggggg ctcgagagcg attatcgccg
      601 gtcggccaga ttcccgccgc cggcaagggt tcctgccctt ctcagccctc gttggcagca
      661 gccagcttga gcaacgggcg ctcggcttcg gcgtcggcgg aggtgacgtg cttcagctct
      721 ccatcgatgg tggcgccctg gtgcatttca agggcctcgt agtgcacgtc gccgacgatg
      781 cgcgccttcg gttgcagctc gagcagctgc gtcgcgacga ccgggccatc gaccttgccg
      841 ttgatgatga cgtggccagc cagtaccttg ccctgcacgc gggcgttctc gctgatgacg
      901 agcaggctct gcgactccga cgccaccacg tcgcccacga cctcgccgtc gatgcgcagg
      961 ccctggtcga agcgcagctc accctgcacg acggtgcctt cgccgatcag gctgcggatg
     1021 ggcggctgct tcttccggtt gaaccacatg gtcacgctcc tctttgtctg cttgccgcgg
     1081 ccgggccgcg tcccaacgaa tcgacgttta tagcggtcgc ccgcgacgag cgcgtttcaa
     1141 agcttgagcg tctgtaacac gcgcacggcg ccgcccgcat cggtgacctt gacctgcacg
     1201 gtttttacca cgacgtccga cgggcaatcg atcatgccct cgacgcgtgc gtattgcttc
     1261 acctgcagcg tctgccgcgt cgccggcgag gacggcgacg tccacggctg gccggcgcgc
     1321 gtgcccgcca gcgtggcctc gtagaagccg ttgaactggg ccggcgtgcg gccgccctgc
     1381 atcaccagca gctggaagcg gacatgtccc ggcgcctgca gctcggcctg gaagccgcgc
     1441 acgaccaggc cctccggcgt gccgccgccc tgcgggatca ggcgctggaa gaagccgagg
     1501 tcggcctgca gcgcgagctt ctcggcctcg ctctggcgca gctgggcggc cagccgctcc
     1561 tcggtgacgc gctcggcggt gagcaggctc tgcgcggtgt tggcgacggc gtccgccttg
     1621 tcgcgctcgg cgcgcaggtc gtcctgctcc gcgcgcagcc gcttcagctc ggccttggca
     1681 tcgcggtcga ggcccgcgag atccttgccc acctcgaacg cccacagcgc ggtcgccgcg
     1741 gagaagccca gcaccagcgc tgccagcagc cagcgcagcg gccacgccag gtggcggcga
     1801 acggtgacgc gcggcgcact gatggacagg cgccgccgca gcagcttcca gcgcatgagc
     1861 ggatgtgact ccggtgaaca gatgaaaacg gacagccgaa agaaaaaagg ccgctgggca
     1921 gcggccttct ccggcaagcc cggcagccag ggccgggcga cgcaagtgcg cgatattagc
     1981 gcttgctgaa ctgcttgcgg cgacgggcgc cgtgcaggcc gaccttcttg cgctcgactt
     2041 cacgggcgtc gcgcgtcacg aagccggcgc ggctcaggtc gcccttcagc gcggcgtcgt
     2101 agtcgatcag cgcgcgggtg atgccgtggc ggacggcgcc ggcctggccg gactcgccgc
     2161 cgccgtgcac gttcaccttg acgtcgaagg ccagttcgtt gttcgtcagc atcaggggtt
     2221 gcttgacgat catgatcgag gtctggcgac cgaagtactc ttcgatggtc ttgccgttga
     2281 cgacgatctt gccctcgccc ttcttgatga agacgcgggc gacggacgac ttgcggcggc
     2341 ccgtcccgta attccaatta ccgatcatcg cggttcctta gatttccagg gccttgggct
     2401 gctgagcggc gtgcgggtgc gtggcaccgg cgtagacctt cagcttcttg atcatcgcgt
     2461 agccgagcgg gcccttgggc agcatgccct tgacggcctt ctccagggcg cggcccggat
     2521 gcttcgcctg catgtccttg aacttggtgg cgtagatgcc gcccgggtag cccgaatggc
     2581 ggtagtacac cttgtcctcg gccttgttgc cggtgacacg gagcttgtcg gcgttgacga
     2641 tgacgatgaa atcgccggtg tccacgtggg gcgtgtagat ggccttgtgc ttgccgcgga
     2701 gtcggtgtgc cacttcactg gcgacacgac cgagcacctt gtcggtggcg tcaatcacaa
     2761 accactcgtg caccacttcg gccggcttgg cgctgaaggt cttcatgagt gttttctcga
     2821 tggaggaaaa gtcgcggccg ttcgacggct ccggcacgtc cgtacgaccc tcggcgctgc
     2881 tcatgtctgg caacctctcg gatccgcacg tgccggcatc ttcttggcca ggcgcccggg
     2941 cggcgcccgt cggacgcccc ccggcgcgcg aggcgccgtc accccgccgc aaaacgggaa
     3001 gcccacgagt atcgcacaaa accggcctgc tgaccaactt cgtcgcggcg gcgagcgatt
     3061 tggggcccgg cgccgacacg acaggggccg gcaggccgcc gccgttgcgt gtacagtctg
     3121 gcttccgggt aaacacccac gcccggacca gcctccgcac acctgcccgc gcctcccacc
     3181 ggcatgaccg ccgccccacc ggaaccgacc ccggacagcg cctcccccgc gcgccgcccg
     3241 cgcgccttcg gctgggtgcc gatccgctcg ctgtcgagcc gccaccgccc ccgcatgctc
     3301 gcccacctgc tggcgttggg catgggcgac cgctacctgc gcttcggcta cgcggcagcc
     3361 gacgaccaga tccgccgcta cgtcgagcgc atcgacttcg accgcgacga ggtcttcggc
     3421 gtcttcaacc gccgcctgga gctgatcgcc ctggcgcacc tcgcctacga gcccgagccg
     3481 gagctcgagc ccgggcaggc ggaacgcccg ctcgccgccg agttcggcgt ctcggtcgcg
     3541 ctcgccgcgc gtggccgcgg ctacggcgcg cggctgttcg accacgcggt gctgcgcgcg
     3601 cgcaaccggg gcgtcgacac catcgtcatc catgcgctgt cggagaacga cgcgatgatc
     3661 cgcatcgttc gcaacgccgg cgcgcacatc gagcacacgg gcggcgactc cgaggctcac
     3721 ctgcgcctgc cgcccgagcc cgaagccggc gccgggcagg aggcggacat cgaggcccgc
     3781 ctggcggaac tcgactacca gttcaagcag ggcgcccgcc acatggacac gatgctggac
     3841 atcggcgccg gcaccgatcc ggcccgttga aggacgaggc gtcacaatcc cggggatgac
     3901 cgccgccccc gacgaccgcg ccccgtacgc ccgcctgacg ccgcaccaga tgctggacgc
     3961 gctcgccgcg accggctttc ccgtgaccgg cgggctgctg caactcaact cgtacgagaa
     4021 ccgcgtcgtg caggccgcgc tggagggcgg cggggcggtc gtcgcgaagt tctaccggcc
     4081 gcggcgctgg agcgactccc agatcctcga ggagcacgac ttcgcccgcg agctcgccgc
     4141 ggacgacc
//
LOCUS       DASVLN010000552         2408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_460146, whole genome shotgun sequence.
ACCESSION   DASVLN010000552 DASVLN010000000
VERSION     DASVLN010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460146"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VF453_13340"
     CDS             <1..343
                     /locus_tag="VF453_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HEX7688689.1"
                     /translation="AGCETAPAPAVGVTDLAERPAERALLGAMRAYDDADYPAVERQA
                     NDALRLGLRSPRDLATAHKLRAFVYCTSNRLAECEAEFRAARAADPAFVLTHAEAGHP
                     VWGPVYLKSRQ"
     gene            545..1000
                     /gene="ybgC"
                     /locus_tag="VF453_13345"
     CDS             545..1000
                     /gene="ybgC"
                     /locus_tag="VF453_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX7688690.1"
                     /translation="MNAILPSTSPGLPAATSVRPAAVFTHPVRVYWEDTDAGGIVFYA
                     NYLKFFERARTEWLRSLGHGQQEMVTATGCMFVVQETRVRYLSPARLDDLLSVTVDVT
                     HRGRASLMIAQQAWCGDRLVAEGEIRIGCVERATMKPQRIPGPILEAIE"
     gene            997..1689
                     /gene="tolQ"
                     /locus_tag="VF453_13350"
     CDS             997..1689
                     /gene="tolQ"
                     /locus_tag="VF453_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX7688691.1"
                     /translation="MNQDLSIIELVAHASTVVQIVIAGLLLMSLFSWGLIFTKLGSLG
                     RIKRRNGAFEEEFWSGKNLNDLYSQASNQADAGPLERLFASGMREFMKLRDKRMDAGA
                     QLDGARRAMRASQQRELDAIEGNLGFLASVGSVSPYVGLFGTVWGIMHAFVGLSNLTQ
                     VTLATVAPGIAEALVATAIGLFAAIPAVVAYNRFARDIDRIATQLDSFSEEFSNILQR
                     NVSGPQGAAGAR"
     gene            1705..2127
                     /locus_tag="VF453_13355"
     CDS             1705..2127
                     /locus_tag="VF453_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HEX7688692.1"
                     /translation="MPGVVARGRGRRSISEINMVPFIDVMLVLLIIFMVSAPLITTGI
                     VDLPTMGKAKQRPPSVIEVIVGTDEHVRIRIDKGDPEPTTVGQLSARVTEMQQGRSDV
                     PVVIAADKTVRYESVVKVMDTLQKAGVQRIGLSVKQGT"
     gene            2180..>2408
                     /locus_tag="VF453_13360"
     CDS             2180..>2408
                     /locus_tag="VF453_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolA"
                     /protein_id="HEX7688693.1"
                     /translation="MTSSILAPRPTDRPPPHEPWGRGVAVSVVAHALLIAALWWGVHW
                     HNNENEGVSAELWAAVPEAAAPPAVAPPPPPP"
BASE COUNT          352 a          913 c          808 g          335 t
ORIGIN      
        1 cgccggctgc gagaccgcgc ctgcgcccgc cgtcggcgtc accgacctcg ccgagcgccc
       61 cgccgagcgc gccctgctgg gtgcgatgcg ggcctacgac gacgccgact accctgcggt
      121 cgagcgccag gccaacgacg cgctgcgcct gggcctgcgc agcccgcgcg acctcgccac
      181 cgcgcacaag ctgcgcgcgt tcgtctactg caccagcaac cggctcgccg aatgcgaggc
      241 ggagttccgc gccgcccggg ccgccgaccc ggccttcgtg ctgacccacg ccgaggccgg
      301 tcatccggtc tggggcccgg tctacctgaa gtcgcgccag tgagcgccaa cgtcgcggtc
      361 caccggcgcc gcgcgctcca gcgcgacgga actcgcgcgg cctgatcggg atcgatcttc
      421 cgcgcgggcg ccggcccgac aattgccggg ccctcgcgcg cctcggcgca gcccgcgcgc
      481 gaagcatccc gacacgcctg ccacggcgga gggacggccc gctcgcttat catcgcgtcg
      541 cccaatgaac gccattctcc cgtccacgtc gcccggcctg cctgccgcga cgtccgtccg
      601 ccccgccgcc gtcttcacgc atcccgtgcg cgtgtactgg gaagacaccg acgccggcgg
      661 catcgtcttc tacgccaact acctgaagtt cttcgagcgc gcccgcaccg agtggctgcg
      721 ctcgctgggc cacggccagc aggagatggt caccgccacc ggctgcatgt tcgtcgtcca
      781 ggagacgcgc gtgcgctacc tgagcccggc gcgcctggat gacctgctgt ccgtcaccgt
      841 cgacgtcacc caccgcgggc gcgcgagcct catgatcgcc cagcaggcct ggtgcggcga
      901 ccgcctggtg gccgagggcg agatccgcat cggctgcgtc gagcgcgcca ccatgaaacc
      961 ccaacgcatc cccggcccca tcctcgaggc gattgaatga accaggatct ctcgatcatc
     1021 gaactcgtcg cgcacgcgag caccgtcgtc cagatcgtca tcgcggggct cctgctgatg
     1081 tcgctgttca gctgggggct gatcttcacc aagctgggct cgctgggccg catcaagcgg
     1141 cgcaacggcg cgttcgagga ggagttctgg tccggcaaga acctcaacga cctctactcg
     1201 caggcgtcca accaggccga tgccggaccg ctcgagcgcc tgttcgcgag cggcatgcgc
     1261 gaattcatga agctgcgcga caagcgcatg gatgccggcg cgcagctgga cggcgcgcgc
     1321 cgcgcgatgc gcgcgagcca gcagcgcgag ctcgacgcga tcgagggcaa cctcggcttc
     1381 ctcgcctcgg tcggctcggt gagcccgtac gtcggcctgt tcggcacggt atgggggatc
     1441 atgcacgcct tcgtgggcct gtcgaacctg acgcaggtca cgctcgcgac ggtcgcgccg
     1501 ggcattgccg aggcgctggt ggccaccgcg atcggcctgt tcgcggcgat cccggcggtc
     1561 gtcgcctaca accggttcgc gcgcgacatc gaccgcatcg cgacccagct ggacagcttc
     1621 agcgaggagt tctccaacat ccttcagcgc aacgtctccg ggccgcaggg cgcggcgggc
     1681 gcgcgctgac cgaggggatc cgccatgcca ggcgtcgtcg cccgcggccg cggccgccgc
     1741 tcgatcagcg agatcaacat ggtgccgttc atcgacgtca tgctggtgct gctgatcatc
     1801 ttcatggtca gcgcaccgct gatcaccacc ggcatcgtcg acctgcccac catgggcaag
     1861 gccaagcagc gcccgccctc ggtgatcgag gtgatcgtgg gcaccgacga gcacgtgcgc
     1921 atccgcatcg acaagggcga ccccgagccg acgaccgtgg ggcagctgtc cgcgcgcgtg
     1981 acggagatgc agcagggccg cagcgacgtg ccggtggtga tcgccgccga caagacggtg
     2041 cgctacgaga gcgtcgtcaa ggtgatggac accctgcaga aggccggcgt gcagcgcatc
     2101 gggctgtcgg tcaagcaggg aacctgagct cgacccggaa gctccacccc gcaggccgcg
     2161 cgtccccgtt catcccgaca tgaccagcag catcctcgcc ccgcgaccga ccgaccgccc
     2221 gccgccgcac gagccctggg gccgcggggt cgcggtgtcg gtggtcgcgc atgccttgct
     2281 gatcgccgcg ctgtggtggg gcgtgcactg gcacaacaac gagaacgagg gcgtgagcgc
     2341 cgagctctgg gccgccgtgc ccgaggccgc ggcgccgccc gcggtcgctc cgccgccgcc
     2401 tccgcccg
//
LOCUS       DASVLN010000553         1564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_514949, whole genome shotgun sequence.
ACCESSION   DASVLN010000553 DASVLN010000000
VERSION     DASVLN010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514949"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VF453_13365"
     CDS             complement(<1..191)
                     /locus_tag="VF453_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688694.1"
                     /translation="MQTILAMLIVLAATGYSVWKLAPASLRRRFAARSGAIARAGGAS
                     ESLARRIEIKAVHAAENAAG"
     gene            complement(192..>1564)
                     /locus_tag="VF453_13370"
     CDS             complement(192..>1564)
                     /locus_tag="VF453_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEX7688695.1"
                     /translation="ALAPPRTVEAGPVGQAAIDAVMADHREVARLIEATVVEPPAAES
                     ATGEALDRVLLHPVWGMAVLAVVLFLVFQAVFSWAKWPMDHIQSAMDGLGHFVAAHMP
                     EGLLRSLVVDGVIAGAGSVLVFLPQILILFTFILALEDSGYLPRAAFLLDRVMASVGL
                     TGRSFIPLLSSFACAIPGIMATRTIADARDRIATILIAPLMTCSARLPVYALLIGAFI
                     PARTLAWGVNLQGLVLFVLYLAGIVSAMTVAWVLQRLGRRNGPQHHTLMMELPAYRWP
                     NLRNLAIGLLERARIFVNRVGTIILALMIVLWFLSTFPSPPPGATGPAIGYSLAGRLG
                     HLLQVVFAPIGFNWQIAIALVPGLAAREVAVGALGTVYALSASGDDVAGQLSPLIAHT
                     WSLPTACALLAWYVFAPQCVATLSVTRRETGGWRHPLIMAGYLFALAYVAAGLTYYAV
                     RHFSGG"
BASE COUNT          260 a          539 c          593 g          172 t
ORIGIN      
        1 ccggcggcgt tctccgcggc gtggacggcc ttgatctcga tgcggcgcgc gagcgattcg
       61 gaggcgccgc ccgcgcgcgc gatcgcgccg gagcgcgcgg cgaagcgccg gcgcagcgac
      121 gccggcgcca gcttccagac gctgtagccg gtggcggcca gcacgatcag catggcgagg
      181 atggtctgca ttcaaccccc ggagaaatgg cgcacggcgt agtaggtcag ccccgcggcg
      241 acgtacgcca gcgcgaagag atagcccgcc atgatcagcg gatggcgcca gccgccggtc
      301 tctcggcgcg tgaccgacag cgtcgcgacg cactgcggcg cgaacacgta ccacgccagc
      361 agcgcgcacg cggtcggcag cgaccaggtg tgggcgatga gcggcgagag ctggccggcc
      421 acgtcgtcgc ccgaggccga caacgcgtag accgtgccca gcgcgcccac cgcgacctcg
      481 cgcgcggcca ggccggggac gagggcgatg gcgatctgcc agttgaagcc gatcggcgcg
      541 aagacgactt gcagcaggtg gcccagccgg cccgcgaggc tgtagccgat ggcgggcccg
      601 gtcgcgcccg gcggcggcga cgggaaggtc gacaggaacc acagcacgat catcagcgcg
      661 aggatgatgg tgcccacgcg gttgacgaag atgcgcgcac gctccagcag gccgatcgcg
      721 aggttgcgca ggttgggcca gcggtaggcc ggcagctcca tcatcagcgt gtggtgctgc
      781 gggccgttgc ggcggcccag gcgctgcagc acccaggcga cggtcatggc gctgacgatg
      841 ccggccaggt acagcacgaa cagcaccagc ccctgcaggt tgacgcccca ggccagcgtg
      901 cgcgccggga tgaaggcgcc gatcagcagc gcgtacaccg gcagccgcgc ggagcaggtc
      961 atcagcggcg cgatgaggat cgtggcgatg cggtcgcgcg cgtcggcgat ggtgcgcgtg
     1021 gccatgatgc cggggatcgc gcaggcgaag ctcgacagca gcgggatgaa gctgcggccc
     1081 gtgaggccga cgctcgccat cacgcgatcg agcaggaagg ccgcgcgcgg caggtagccc
     1141 gagtcttcca gcgcgaggat gaacgtgaac aggatcagga tctgcggcag gaacacgagc
     1201 acgctgcccg cgcccgcgat cacgccgtcg acgacgaggc tgcgcagcag gccctcgggc
     1261 atgtgcgcgg cgacgaagtg gcccagtccg tccatcgccg actggatgtg gtccatgggc
     1321 cacttggccc agctgaagac cgcctggaag accaggaaca gcacgaccgc cagcaccgcc
     1381 atgccccaga ccgggtgcag caggacgcgg tcgagcgcct cgcccgtcgc cgactccgcg
     1441 gccgggggct cgacgaccgt ggcctcgatc aggcgcgcga cctcgcggtg gtcggccatc
     1501 acggcgtcga tcgccgcctg gccgacgggg cccgcttcga cggttcgcgg cggcgcgagc
     1561 gccg
//
LOCUS       DASVLN010000554         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_190204, whole genome shotgun sequence.
ACCESSION   DASVLN010000554 DASVLN010000000
VERSION     DASVLN010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190204"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1672
                     /locus_tag="VF453_13375"
     CDS             56..1672
                     /locus_tag="VF453_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7688696.1"
                     /translation="MSESAAPAAAASASPASPPAPAPAAVRRPLTGSALVMGTLSLSL
                     ATFMNVLDSSIANVSLPAISGDLGVSTSQGTWVITSFGVANAISVPLTGWLTQRFGAV
                     RLFTGSILLFVVASLLCGLAPSIEMLIAFRVLQGFVAGPMIPLSQTLLLSTYPPHKAG
                     TALALWGVTTLVAPVVGPLLGGWLTDDYAWPWIFYINIPVGLFAAGVTWSIYKTRETP
                     TVKRPIDGIGLAMLVVWVGSLQVMLDKGKELDWFNSSTIVTLAVVAVIAFAVFIAWEL
                     TDEHPIVDIRLFKGRNFLFGAGTLAIAYGLFFGNVVLLPLWLQQYMGYTATSAGMALA
                     PVGVLAIMMTPFVGRNVQKWDPRWMATGAFILFSLVLWMRSNFTAQTDIGTILIPTFI
                     QGGAMALFFIPLTTITLSGLPPERIPAAAGLSNFVRITLGAMGTSIATTIWDNRATLH
                     HARLVEQMGLNGTQLDAATRTLSQVGIAGNAGYASINRLIDVQAFTWAADDVFIGSAV
                     TFLFLIFTIWITKRPPKIAGGPSVDAGGAH"
     gene            complement(1703..2131)
                     /locus_tag="VF453_13380"
     CDS             complement(1703..2131)
                     /locus_tag="VF453_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7688697.1"
                     /translation="MTITNAATAANTGADHPATVRRDQPVSFAAKAALLAEQWTPRVV
                     AELNDIQFKVVRIEGDFLWHDHADTDEAFLVLDGVLRIDFRDRQVTVRPGELFVVPRG
                     VEHKPYAEREVRMVLIEPRGVVNTGGLEANERTQANDVWI"
     gene            complement(2203..>2563)
                     /locus_tag="VF453_13385"
     CDS             complement(2203..>2563)
                     /locus_tag="VF453_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7688698.1"
                     /translation="TAAGTPERPVRGDFQAAQRARAHVHANLGEPITLDALAAAAGRD
                     RWSLARDFRAFFGTSPHRYVTRRRLELARRLMIAGVPLAECAVHAGFADQSHLTRHFV
                     QAWGVTPARWLRMARRG"
BASE COUNT          328 a          900 c          916 g          419 t
ORIGIN      
        1 gcctcccgcg cgaggtcgtc cgcctcttac tcccgctacg gaaaggtccg ccaccatgag
       61 cgaatccgca gcaccggccg cggcggccag cgcgtcgccg gccagtcctc ccgccccggc
      121 gcccgccgcc gtccgccgcc cgctgaccgg cagcgccctc gtgatgggca cgctatcgct
      181 gtcgctggcg acgttcatga acgtgctcga ctcgtcgatc gccaacgtgt cgctgcccgc
      241 tatctcgggc gacctgggcg tgagcacctc gcagggcacc tgggtcatca cgagcttcgg
      301 cgtcgccaac gcgatctcgg tgccgctgac cggctggctc acgcagcgct tcggcgccgt
      361 gcggctgttc acggggtcga tcctgctgtt cgtcgtcgcg tcgctgctgt gcggcctcgc
      421 gccgtcgatc gagatgctga tcgcgttccg cgtcctgcaa ggcttcgtcg cggggccgat
      481 gatcccgctc tcgcagacgc tgctgctgtc cacctatccg ccgcacaagg cgggcacggc
      541 gctcgcgctg tggggcgtga cgacgctggt cgcgcccgtg gtcgggccgc tgctgggcgg
      601 ctggctgacg gacgactacg catggccttg gatcttctac atcaacatcc cggtcggcct
      661 gttcgccgcc ggcgtgacgt ggagcatcta caagacccgc gagacgccca ccgtgaagcg
      721 cccgatcgac ggcatcggcc tcgcgatgct ggtggtctgg gtcggctcgc tgcaggtcat
      781 gctggacaag ggcaaggaac tcgactggtt caactcgtcg acgatcgtca cgctcgccgt
      841 cgtggccgtg atcgcgttcg cggtgttcat cgcctgggag ctgaccgacg agcacccgat
      901 cgtcgacatc cgcctgttca agggccgcaa cttcctgttc ggcgcgggga cgctcgcgat
      961 cgcctacggc ctgttcttcg gcaacgtggt gctgctgccg ctgtggctgc agcagtacat
     1021 gggctacacg gcgacgtcgg cggggatggc gctggcgccg gtgggcgtgc tcgccatcat
     1081 gatgacgccc ttcgtcggcc gcaacgtgca gaagtgggac ccgcgctgga tggcgaccgg
     1141 tgcgttcatc ctgttctcgc tggtgctgtg gatgcgctcg aacttcaccg cgcagaccga
     1201 catcggcacc atcctgatcc cgaccttcat ccagggcggc gcgatggcgt tgttcttcat
     1261 cccgctgacg accatcacgc tgtcgggcct gccgcccgag cgcatcccgg cggccgccgg
     1321 cctgtcgaac ttcgtgcgga tcacgctcgg cgcgatgggc acgtcgatcg cgacgacgat
     1381 ctgggacaac cgcgccacgc tgcaccacgc gcggctggtc gagcagatgg gcctgaacgg
     1441 cacccagctc gacgccgcga cccgcacgct gtcgcaggtc ggcatcgccg gcaacgcggg
     1501 ctacgcgagc atcaaccggc tgatcgacgt ccaggccttc acgtgggccg cggacgacgt
     1561 cttcatcggc tcggccgtca ccttcctgtt cctgatcttc acgatctgga tcacgaagcg
     1621 gccgccgaag atcgcgggcg gcccgtcggt ggacgccggc ggcgcgcact gagcgcccgg
     1681 cggcaggcgg cgggtcgcgg cgtcagatcc agacgtcgtt ggcctgcgtc cgctcgttgg
     1741 cttcgaggcc gccggtgttg acgacgccac gcggctcgat cagcaccatg cggacctcgc
     1801 gctcggcgta gggcttgtgc tcgacgccgc gcggcacgac gaagagctcg cccgggcgca
     1861 cggtcacctg gcggtcgcgg aagtcgatgc gcagcacgcc gtccagcacc aggaaggcct
     1921 cgtcggtgtc ggcgtggtcg tgccagagga agtcgccctc gatgcgcacg accttgaact
     1981 ggatgtcgtt gagctccgcg acgacgcgcg gcgtccactg ctcggccagc agggcggcct
     2041 tggcggcgaa cgacacgggc tggtcgcggc gcacggtggc ggggtggtcg gcgccggtgt
     2101 tcgcggcggt ggcggcgttg gtgatggtca tgggagccct cgggttgtcg atggccgact
     2161 ctaggcagcg cggccgcggc gcgtcttgca cgtttgtgcg cttcagccgc gccgcgccat
     2221 gcgcagccag cgcgcgggcg tcacgcccca ggcctggacg aagtgccgcg tcaggtggct
     2281 ctggtcggcg aagccggcgt gcacggcgca ttccgccagc ggcacgccgg cgatcatcag
     2341 gcggcgtgcg agctcgagcc ggcgccgcgt gacgtagcgg tgcgggctgg tgccgaagaa
     2401 ggcgcggaag tcgcgcgcga ggctccagcg gtcgcgcccc gcggcggcgg ccagggcgtc
     2461 cagggtgatc ggctcgccca ggttcgcatg cacatgcgcg cgggcacgct gcgcggcctg
     2521 gaagtcgccg cggacggggc gctcgggcgt gccagccgcc gtc
//
LOCUS       DASVLN010000555         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_190278, whole genome shotgun sequence.
ACCESSION   DASVLN010000555 DASVLN010000000
VERSION     DASVLN010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190278"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VF453_13390"
     CDS             complement(<1..462)
                     /locus_tag="VF453_13390"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688699.1"
                     /translation="MKQLQGLLALIEIAAAGSLAGAARRLGVTPAAVSKNLARLEQQL
                     GVRLVQRSTRTTRITAEGERFIAKARDALRLLDDAVTEVSQAADAPAGTVRISVGVGF
                     GRSWVLPALPVIARRFPQLAIEVELDNAPVDLVARGIDIGIRGGSVPDAGFV"
     gene            588..1223
                     /locus_tag="VF453_13395"
     CDS             588..1223
                     /locus_tag="VF453_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7688700.1"
                     /translation="MKIGIIGSGAVAQTLAAGFLVHGHEVALGTRDAGKLAEFAARHP
                     AARIVGMREAAAFGELAVLAVKGSAALQAARAAGDALDGKPVLDACNPIADAPPTDGV
                     LAYFTGSDESLMEQLQRELPKARFVKAFNSVGAGRMIDPDFAAGRPTMFIAGNDADAK
                     AAAARLLAEVGWDAADMGTAVAARAIEPLAMLWCIPGFREGSWMHAFKLLR"
     gene            complement(1228..>2388)
                     /locus_tag="VF453_13400"
     CDS             complement(1228..>2388)
                     /locus_tag="VF453_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HEX7688701.1"
                     /translation="HGGVCRLALTDADRDGRDRLVAWMRELGLDVRVDRIGNIFGLRA
                     GTRPELAPVMTGSHLDTVATGGRYDGLYGVMAGLEVVRELGERGAATARTLVVAAFTN
                     EEGVRFAPDMMGSLVYAGGYPLEAALDTVGTDGARLGDELARIGYAGAMDCGAIVPHA
                     FVELHIEQGPVLEAEHRAIGAVADLQGISWREIAITGQSNHAGTTPMRLRRDAGHCAA
                     AIAVFVRELARRFGGAQVATVGRLELHPNLINVIAARATLTVDLRNTDEAALRRAESE
                     LDDFLARLQAEEGVAIASRPLARFAPVAFDERLVRRIESAARARGHHALRMTSGAGHD
                     AQMMARICPAAMIFVPSAGGISHNPREHTAPAELALGASVLMDVLLELADET"
BASE COUNT          304 a          898 c          868 g          318 t
ORIGIN      
        1 gacgaagccg gcgtcgggca ccgagccgcc gcggatgccg atgtcgatgc cgcgcgcgac
       61 gaggtcgacc ggcgcgttgt cgagctcgac ctcgatcgcc agctgcggga agcgccgcgc
      121 gatcacgggc agcgccggca gcacccagct gcggccgaag cccacgccga ccgagatccg
      181 taccgtgccc gccggcgcgt cggcggcctg cgagacctcg gtcacggcgt cgtcgagcag
      241 ccgcagcgcg tcgcgcgcct tggcgatgaa gcgctcgccc tcggccgtga tgcgcgtggt
      301 gcgcgtgctg cgctggacga gccgcacgcc cagctgctgc tccagccgcg cgaggttctt
      361 gctgaccgcc gcgggcgtca cgcccaggcg ccgggcggcg ccggcgaggc tgccggcggc
      421 cgcgatctcg atgagggcga gcagcccctg cagttgcttc atgcgcggga ttctcaaccg
      481 gaagtcgctg ctccatcaac tgccgatgac tttctggatc gaccgcgact gcctacagtt
      541 cgtcccatgc ctgcagcggt gcgggcgcca ccccaaggag tttccccatg aagatcggca
      601 tcatcggttc cggcgccgtc gcgcagacgc tcgcggcagg cttcctcgtc cacggtcacg
      661 aggtcgcgct cggcacgcgc gacgcgggca agctggccga gttcgcggcc aggcatccgg
      721 cggcgcgcat cgtcggcatg cgcgaggccg ccgccttcgg cgagctcgcg gtgctggcgg
      781 tcaagggctc ggccgcgctg caggccgcgc gcgcggccgg cgacgcgctg gacggcaagc
      841 cggtgctcga cgcctgcaac ccgatcgccg acgccccgcc gacggacggc gtgctcgcct
      901 acttcaccgg ctccgacgag tcgctgatgg agcagctgca gcgcgagctc ccgaaggcgc
      961 gcttcgtcaa ggcgttcaat tccgtcggcg cgggccgcat gatcgatccc gacttcgccg
     1021 cgggccgccc gacgatgttc atcgccggca acgacgcgga cgccaaggcc gccgccgcgc
     1081 gcctgctggc cgaggtcggc tgggacgcgg ccgacatggg caccgccgtc gccgcccgcg
     1141 ccatcgagcc gctggccatg ctctggtgca tcccgggctt ccgcgagggg tcgtggatgc
     1201 acgcgttcaa gctgctgcgc tgaagcttca ggtctcgtcc gccagctcca gcagcacgtc
     1261 catcagcacg ctcgccccca gcgcgagctc cgccggcgcg gtgtgctcgc gcgggttgtg
     1321 gctgatcccg ccggcgctgg gaacgaagat catggccgcg gggcagatcc gcgccatcat
     1381 ctgcgcgtcg tggccggcgc ccgaggtcat gcgcagcgcg tgatggccgc gggcgcgggc
     1441 ggccgactcg atgcggcgca ccaggcgctc gtcgaaggcc accggcgcga agcgcgcgag
     1501 cggccgcgac gcgatcgcca cgccttcctc ggcctgcagg cgtgcgagga agtcgtcgag
     1561 ctcggattcg gcgcggcgca gcgccgcctc gtcggtgttg cgcaggtcga cggtcagcgt
     1621 cgcgcgcgcc gcgatcacgt tgatcaggtt gggatgcagc tcgaggcggc cgactgttgc
     1681 gacctgcgcg ccgccgaagc gccgcgccag ctcgcgcacg aacaccgcga tcgcggccgc
     1741 gcagtggccg gcgtcgcggc gcaggcgcat cggcgtcgtg ccggcgtggt tcgactgccc
     1801 cgtgatcgcg atctcgcgcc acgagatgcc ctggaggtcg gccaccgcgc cgatcgcgcg
     1861 gtgctcggcc tccagcaccg ggccctgctc gatgtgcagc tcgacgaacg cgtggggcac
     1921 gatcgcgccg cagtccatcg cgccggcgta gccgatgcgc gcgagctcgt cgcccagcct
     1981 cgcgccgtcg gtgccgaccg tgtccagcgc ggcttccagc ggatacccac ccgcgtacac
     2041 cagcgagccc atcatgtcgg gcgcgaagcg caccccttcc tcgttggtga acgccgcgac
     2101 gaccagcgtg cgcgccgtcg cggcgccgcg ctcgcccagc tcgcgcacga cctccaggcc
     2161 ggccatcacg ccgtagaggc cgtcgtagcg cccgcccgtg gcgacggtgt cgaggtgcga
     2221 gccggtcatc acgggcgcga gttccggccg cgtgcccgcg cgcaggccga agatgttgcc
     2281 gatgcgatcg acgcgcacgt ccagcccgag ctcgcgcatc caggcgacga ggcggtcgcg
     2341 gccgtcgcgg tcggcgtcgg tgagcgcgag ccggcacacg ccgccgtg
//
LOCUS       DASVLN010000556         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_405086, whole genome shotgun sequence.
ACCESSION   DASVLN010000556 DASVLN010000000
VERSION     DASVLN010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405086"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VF453_13405"
     CDS             complement(<1..449)
                     /locus_tag="VF453_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688702.1"
                     /translation="MKLSPSSSRGSLVHRITVTGVVAVLACAGVLGTVATLLARHTLE
                     QQAQEKLVVVGDAASMRIGEWVAGNLRTTGSVSAAVTPDAIAKALTESTHAGFAHRRG
                     ASAHPAPRAARMDVVALAMKEPTRAGDDKSKPDAAAAAALAAIAGIG"
     gene            719..1351
                     /locus_tag="VF453_13410"
     CDS             719..1351
                     /locus_tag="VF453_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX7688703.1"
                     /translation="MELDPLELVPEPSGFGDDDDSPPTPAPARARARLNAAVAITVAL
                     LATFMGICKIKDDNICQAMQQAQADKIDHWSYYQARNVRQEVAEAAAVQLRVQQAGAP
                     ATGQAAAAEAAGRYDAIAKEQAQKKEELRRQAVADQASYDALNYRDDQFDLSDALLAI
                     AISMLAVSSLTQLLWLYLLSLVPAGFGVLMGVAGLAGLHIHPDRLTQLLS"
     gene            1453..2328
                     /locus_tag="VF453_13415"
     CDS             1453..2328
                     /locus_tag="VF453_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7688704.1"
                     /translation="MKLFRHGPRGHERPGLVDHDGIPRDLSAQIGDIGPADLSPERLA
                     RLRSVVPSALPALAPGFRYGVPWHGMSKFVCIGLNYTDHAAESGMELPREPIVFMKAT
                     SALTGPDDPIVLPPDAAKGDWEVELGVVIGTRARHVAEADALAHVAGYCVVNDVSERA
                     YQLERGGTWDKGKGCDTFGPVGPWLVTADEVPDPQALALWLDVGGERMQDGSTGKMVF
                     GVAAIVAYVSRFMTLEPGDLIATGTPAGVGMGRKPPRFLRPGDTLRLGISGLGEQRTT
                     VHAWDTALVDRVHGR"
     gene            2606..2965
                     /locus_tag="VF453_13420"
     CDS             2606..2965
                     /locus_tag="VF453_13420"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEX7688705.1"
                     /translation="MNTSLRVALAAAALLASPLALARTALVASLPANGQALDASPTEI
                     RLTFNEHVDARGASVKLVSAKGQHWDCDRPHGDKSDANALVATVPVLRSGSYRARWTA
                     VAPDGRKMHGDLNFSIK"
BASE COUNT          440 a         1093 c         1067 g          381 t
ORIGIN      
        1 ttgccgatgc cggcgatcgc cgccagcgcg gccgcggcgg cggcgtccgg cttggacttg
       61 tcgtcgccgg cccgggtggg ctccttcatg gccagtgcga cgacgtccat gcgcgcggcg
      121 cgcggcgcgg gatgcgcgga ggcgccgcgg cgatgggcga agccggcgtg cgtggactcg
      181 gtcagcgcct tggcgatggc gtccggcgtg accgcggcgc tcacgctgcc ggtcgtgcgc
      241 aggttgcccg ccacccattc gccgatgcgc atcgacgcgg cgtcgccgac gaccaccagc
      301 ttctcctgcg cctgttgctc gagcgtatgg cgggccagca gcgtggccac ggtgccgagg
      361 acgcctgcgc aggcgagcac ggcgacgacg ccggtgacgg tgatgcgatg gacgaggctg
      421 ccgcgggacg acgacgggga gagtttcatg gcgacgcttt cggatgtggg ctcggactgg
      481 atgtttctgg cggtcttttt cgggaggcga gacaagactt cggccggacc gcgcggacct
      541 gtagggggat cttgacgcgc gggcgtcgcg gccaagcgcg gaaaaacacc ggaaaacccg
      601 tccggatgca cgccacggcc cgattcgcgc gggaaacggc cgaccgggcg ccgcgcaaac
      661 cgaacgcaag ccaggacggg tacgctgtcc gcccccgcat ccaccgcccc tctccttcat
      721 ggaactcgac ccgctggaac tggtgccgga gccctcgggc ttcggcgacg acgacgactc
      781 cccgccgacg ccagcgcccg cccgcgcccg cgcgcgcctg aacgcggccg tcgcgatcac
      841 cgtcgccctg ctcgccacct tcatggggat ctgcaagatc aaggacgaca acatctgcca
      901 ggcgatgcag caggcgcagg ccgacaagat cgaccactgg agctactacc aggcgcgcaa
      961 cgtccgccag gaggtcgccg aggccgcggc ggtgcagctg cgcgtgcagc aggcgggcgc
     1021 gccggcgacc ggccaggccg cggccgccga ggccgccggc cgctacgacg ccatcgccaa
     1081 ggagcaggcg cagaagaagg aggagctgcg caggcaggcc gtcgccgacc aggcgagcta
     1141 cgacgcgctc aactaccgcg acgaccagtt cgacctgtcg gacgcgctgc tggcgatcgc
     1201 gatctcgatg ctggcggtct cctcgctcac gcagctgctc tggctgtacc tgctgagcct
     1261 cgtgccggcc ggcttcggcg tgctgatggg cgtggccgga ctcgccgggc tgcacatcca
     1321 tcccgaccgg ctcacgcagt tgctcagctg acggctgcca cagccgcgaa ccccggggtc
     1381 gccgacatgg gcatcggcct tgccgcacgc tcgcgtcctc gcgttctcca cacggccccg
     1441 aggcccgacc gcatgaaact cttccgccac ggcccgcgcg gccatgaacg tcccggcctc
     1501 gtcgaccacg acggcatccc gcgcgacctc agcgcccaga tcggcgacat cggccccgcc
     1561 gacctgtcgc ccgagcgcct cgcccgcctg cgctcggtgg tgccgtcggc gctgccggcg
     1621 ctcgcgccgg gcttccgcta cggcgtaccg tggcacggca tgtccaagtt cgtctgcatc
     1681 ggcctgaact acaccgacca cgcggccgag tcgggcatgg aactgccgcg ggagcccatc
     1741 gtcttcatga aggcgacgag cgcgctcacc ggcccggacg acccgatcgt gctgccgccg
     1801 gacgcggcca agggcgactg ggaggtcgag ctgggcgtgg tgatcggcac ccgcgcgcgc
     1861 catgtggccg aggcggacgc gctggcgcac gtcgcgggct actgcgtcgt gaacgacgtc
     1921 tcggaacgcg cgtaccagct cgagcgcggc ggcacctggg acaagggcaa gggctgcgac
     1981 accttcggcc cggtcggtcc gtggctcgtg acggcggacg aagtccccga cccgcaggca
     2041 ctcgcgctgt ggctggacgt cggcggcgag cgcatgcagg acggctcgac cggcaagatg
     2101 gtgttcggcg tggcggcgat cgtcgcgtac gtgagccgct tcatgacgct ggagcccggc
     2161 gacctgatcg ccaccggcac gcccgcgggc gtcggcatgg gccgcaagcc gccgcgcttc
     2221 ctgcgccccg gcgacacgct gcgactgggc atctcggggc tcggcgaaca gcgcacgacc
     2281 gtgcatgcct gggacacggc gctggtcgac cgcgtccacg gacgctagac gcaccgcttc
     2341 gtccggccaa ccgccggccg gcggctgcgc gacgcggact ggtacgggtg gccaccgcgc
     2401 acggtcgcgc acgaatggac ggaatggacg ggcgagggcc cgcgccgcgc gcccgggctg
     2461 acttgcgcga tcgcaacact ccgccggcgg aagtcgaagt ccaggcgcgg aatccggcca
     2521 gggccggcat gcgcatggaa cactggcgca tcgccgcggt cgaacgggcc gccgccacga
     2581 caacgccaag acgggagatc cggacatgaa tacttcgctg cgcgtcgcgc tcgccgccgc
     2641 ggccctgctc gccagtcccc tcgcgctcgc acgcaccgcg ctggtcgctt cgctgcccgc
     2701 gaacggccag gcgctggacg cgtcgcccac ggagatccgg ctgaccttca acgagcacgt
     2761 cgacgcgcgc ggtgcctcgg tcaagctggt gagcgcgaag ggccagcact gggactgcga
     2821 ccgtccgcac ggcgacaagt ccgacgccaa tgcgctcgtc gcgacggtgc cggtgctgcg
     2881 cagcggcagc taccgcgcgc gctggacggc cgtcgcgccc gacggccgca agatgcacgg
     2941 cgacctgaac ttctcgatca agtagccgaa tcgcgacacg g
//
LOCUS       DASVLN010000557         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_138286, whole genome shotgun sequence.
ACCESSION   DASVLN010000557 DASVLN010000000
VERSION     DASVLN010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138286"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..813
                     /locus_tag="VF453_13425"
     CDS             289..813
                     /locus_tag="VF453_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7688706.1"
                     /translation="MDMIKEATHIARNATERTNAAVKGGAKGEFLTFRLGGEEYGIDI
                     LRVQEIRSYEQPTRIANTPEFLRGVVNLRGVIVPIVDLRLKLSCQSADFNAFTVVIVL
                     NVKGRVIGAVVDSVSDVCELGGDMIKPAPTLNSHVDMSFITGIANVGERMLILMDIEA
                     LMSASDMGLIESAA"
     gene            971..2275
                     /locus_tag="VF453_13430"
     CDS             971..2275
                     /locus_tag="VF453_13430"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7688707.1"
                     /translation="MPDLIVHGGLPLSGRITPSANKNAVLPILSATLLTREPVTLRGV
                     PDITDVRKILAVFRELGSTVEGELASGVLRIHHERTNFDPAVHKLPEEMRSSVMLVPP
                     LLARFGCARIENDVQGCTLGVREIDPHVEVFEGFGAQVERGKGALHVTAPRPLHAHRQ
                     WLDYASVTTTENFVLCAALADGVSELTNAASEPHVQEFCKFMREMGAKVEGCGTSKLT
                     VTGVDALRGVDFTFVEDFHEVVTFLALGAITGGDVEVRNSAPQFFPLIDRSFAKLGVE
                     VVHEGGWSRARVPGGLKVREPFTRNMLQKIEAAPWPYFPVDLLPIFIALGVRAEGQVM
                     FWNKVYDGALGWTSELSKFGAHAFLSDPHRVITFGGKPLVAAEVESPYIIRVAIALLM
                     LASSIDGRSTIYNATPIRRAHPRFVENLVSLGAKIEWVGADA"
     gene            complement(2336..2740)
                     /locus_tag="VF453_13435"
     CDS             complement(2336..2740)
                     /locus_tag="VF453_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX7688708.1"
                     /translation="MTVRIVRLGTPRAPHEGLRIGTVRRPPRGVRKERFAQDDWYDVW
                     LPALSPSAELVKRALEAQKDDSPAAEREWTSFVRKFRAEMARSEPAQTIALLAALSRD
                     ADFSVGCYCEDEARCHRSVLRALLEEAGAKVA"
     gene            complement(2745..3215)
                     /locus_tag="VF453_13440"
     CDS             complement(2745..3215)
                     /locus_tag="VF453_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7688709.1"
                     /translation="MIRIGLISDTHGLLRPEALAALRGSDFIVHAGDIGEGILERLAT
                     IAPVTAVRGNNDRGAWAGRLADKEWLQVGEVVVHVLHDIADLALDPSGAGVDVVVTGH
                     SHRPRVERRGGTLFVNPGSAGPVRFRLPVSVGMLQVTGREIQARIVELESLRVS"
     gene            complement(3320..>4443)
                     /locus_tag="VF453_13445"
     CDS             complement(3320..>4443)
                     /locus_tag="VF453_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7688710.1"
                     /translation="ATRAIAARPAFAVLQVASMAVGLLALWVLVLLRTDLIDSWRAAT
                     PPGAPDRFVINIQPNQGDAYRAALKAAGVRHDDWFPMIRGRLLTINGEPAAPRYEHDA
                     NALRMVEREFNLSHTLELPDDNRIVAGRWIPGEPDGASMEEGLAKTLGVKLGDTLVFG
                     VGGQQFTSRITSLRKVDWGSMRVNFFVLFPRADMGDVPYSYISAFKAPHAPGFDNRLA
                     AAFPNLTVFDVSAALAQVQGVLDQVVHAVELLFGFALVAGLVVLMATVSATREARARE
                     FAVMRAVGASATLLSRVQRAELLGVGALAGFLAATAALVVGWLLASQVFEFHWNPPWW
                     VPLAGAASGGVLALAAGIRSLRDVLARPVMETLRRAAQE"
BASE COUNT          665 a         1630 c         1532 g          616 t
ORIGIN      
        1 ggccaagccc gcgccggtcg ccgcctccac gcccgctccg acccccgccc cggccatggc
       61 cgtcggcgcg agcgacgact gggtatcgtt ctgaggacga ctccctcccc gacgggcgcc
      121 ggcgaggcgc cctttttttg cccgcgcggg cggcccgcaa cccccgccga ccgcgccaaa
      181 agtcacttga ttccgcccgg ccgcggccga taacggatca gagacccgcc ttcatccgcc
      241 accccgcgtc gtccgcgacg cccgcacgca aacgccagag gatccgccat ggacatgatc
      301 aaggaagcca cccacatcgc ccgcaacgcc accgagcgca cgaacgcagc ggtcaagggc
      361 ggcgccaagg gcgagttcct gaccttccgt ctcggcggcg aggagtacgg catcgacatc
      421 ctgcgcgtgc aggagatccg ctcgtacgag cagccgaccc gcatcgccaa cacgcccgag
      481 ttcctgcgcg gcgtcgtcaa cctgcgcggc gtgatcgtgc cgatcgtcga cctgcgcctg
      541 aagctgtcgt gccagagcgc cgacttcaac gcgttcacgg tcgtgatcgt gctgaacgtc
      601 aagggtcgcg tgatcggcgc ggtggtggac tcggtctcgg acgtctgcga gctgggcggc
      661 gacatgatca agccggcgcc gacgctgaac tcgcacgtcg acatgtcgtt catcacgggc
      721 atcgccaacg tcggcgagcg catgctgatc ctgatggaca tcgaggcgct gatgagcgcg
      781 tcggacatgg gcctgatcga atccgcggcc tgagcgaccc ctcgtcacga cagccgagcc
      841 gcctgcgggc ggctttcggc ttttcgggcc ggagcaagcg cgacgccgcg cggaagaaag
      901 tctcacgacg ccggccgccg cgggctctta agatgccggc acgtcgtttc ccctgtcctg
      961 aaagcatctc gtgcccgacc tcatcgtcca cggcggcctg cccctctcgg gccgcatcac
     1021 gccctccgcc aacaagaacg cggtgctgcc catcctgtcg gccacgctcc tgacgcgcga
     1081 gcccgtcacc ctgcgcggcg tgcccgacat cacggacgtg cgcaagatcc tcgccgtctt
     1141 ccgcgaactg ggcagcacgg tcgagggcga gctcgcgtcg ggcgtgctgc gcatccatca
     1201 cgagcgcacg aacttcgatc ccgccgtgca caagctgccc gaggagatgc gctcgtccgt
     1261 gatgctggtg ccgccgctgc tggcgcgatt cggctgcgcg cgcatcgaga acgacgtcca
     1321 gggctgcacg ctcggcgtgc gcgagatcga cccgcacgtg gaagtcttcg aaggcttcgg
     1381 cgcccaggtc gagcgcggca agggcgcgct gcacgtgacc gcgccgcggc cgctgcacgc
     1441 gcatcgccag tggctcgact acgcctcggt cacgacgacc gagaacttcg tgctgtgcgc
     1501 ggcgctcgcc gacggcgtct ccgagctgac caacgccgcc tccgagccgc acgtgcagga
     1561 gttctgcaag ttcatgcgcg agatgggcgc gaaggtcgag ggctgcggca cctcgaagct
     1621 gaccgtcacc ggcgtcgacg cgctgcgcgg cgtcgacttc accttcgtcg aggatttcca
     1681 cgaggtcgtc accttcctcg cgctgggcgc gatcacgggc ggcgacgtcg aggtgcgcaa
     1741 cagcgcgccg cagttcttcc cgctgatcga ccgcagcttc gccaagctgg gcgtcgaggt
     1801 cgtgcacgag ggcggctggt cgcgcgcccg cgtgcccggc ggcctgaagg tgcgcgagcc
     1861 cttcacgcgc aacatgctgc agaagatcga ggccgcgccg tggccctact tcccggtcga
     1921 cctgctgccc atcttcatcg cgctgggcgt gcgcgccgag gggcaggtga tgttctggaa
     1981 caaggtctac gacggcgcgc tcggctggac gtcggagctg tcgaagttcg gcgcccacgc
     2041 cttcctgtcg gaccctcacc gcgtcatcac cttcggcggc aagccgctgg tggcggccga
     2101 ggtcgagagt ccgtacatca tccgcgtcgc gatcgcgctg ctgatgctcg cctcgagcat
     2161 cgacggccgc tccacgatct acaacgcgac gccgatccgc cgcgcgcatc cgcgcttcgt
     2221 ggagaacctg gtgtcgctgg gggcgaagat cgagtgggtg ggcgcggacg cctgagggcg
     2281 ccgccgcgcg gcggcgcgtc cgccgccggc atcgggcggg tcgcgttccg gacgatcacg
     2341 ccaccttcgc ccccgcttct tccagcaacg cccgcagcac cgagcggtgg cagcgggcct
     2401 cgtcctcgca gtagcagccg accgagaagt ccgcatcgcg cgacagcgcg gccagcagcg
     2461 cgatcgtctg cgccggctcg gaccgggcca tctccgcgcg gaacttgcgc acgaaggacg
     2521 tccactcgcg ctccgcggcg ggcgagtcgt ccttctgcgc ctccagcgcc cgcttgacca
     2581 gctccgcgct cggcgaaagc gccgggagcc acacgtcgta ccagtcgtcc tgcgcgaagc
     2641 gctccttgcg gacgccccgc ggcgggcggc ggaccgtgcc gatgcgcagg ccctcgtgcg
     2701 gagcgcgtgg ggtgccgagg cggacgatgc ggacggtcat gacgtcacga tacccgcagc
     2761 gactcgagct ccacgatgcg ggcctggatc tcgcgacccg tcacctgcag catcccgacc
     2821 gagaccggca gccggaagcg caccggcccc gcgctgccgg ggttgacgaa cagcgttccg
     2881 ccacggcgct cgacccgggg cctgtgcgag tgtccggtga cgacgacgtc cacgcccgcc
     2941 ccggagggat cgagcgccag gtcggcgatg tcgtgcagca cgtgcacgac cacctcgccg
     3001 acctgaagcc actccttgtc cgccaggcgc ccggcccacg cgccgcggtc gttgttccct
     3061 cgcacggcgg tcacgggcgc gatcgtggcg agacgctcga ggatgccctc gccgatgtcg
     3121 cccgcgtgga cgatgaagtc gctgcctcgc agcgccgcga gcgcctcggg ccgcagcagg
     3181 ccgtgggtgt cggagatgag gccgatccgg atcatggaag ttgtcgcatc aaggcgcgtg
     3241 cccggacgat accgccgcat gcacgcaagt gcgatgacgg tgcttcgagg gtggacggcg
     3301 cgccgctgcg gacgggtcgc tattcctgcg cggcgcgccg cagcgtctcc atcactggtc
     3361 gcgccaggac gtcgcgcagg ctgcggatgc cggccgcgag cgcgagcacg ccgccgctgg
     3421 ccgcgccggc gagcggcacc caccagggcg gattccagtg gaactcgaag acctgggagg
     3481 cgagcagcca gccgaccacc agcgccgcgg tcgcggcgag aaagcccgcg agcgcgccga
     3541 cgcccagcag ctcggcgcgc tgcacgcgcg acagcagcgt ggcggatgcg ccgaccgcgc
     3601 gcatcacggc gaactcacgc gcccgcgcct cgcgcgtcgc cgagaccgtg gccatcagca
     3661 cgaccagccc cgccacgagg gcgaagccga acagcagttc caccgcgtgc acgacctggt
     3721 cgagcacacc ctggacctgc gcgagcgcgg ccgagacatc gaacaccgtg aggttgggga
     3781 acgccgcggc gagccggttg tcgaagccgg gggcatgcgg cgccttgaac gcgctgatgt
     3841 agctgtacgg cacgtcgccc atgtccgcac gcgggaacag cacgaagaag ttgacgcgca
     3901 tcgagcccca gtcgaccttg cgcaggctgg tgatgcggct ggtgaactgc tggccgccga
     3961 cgccgaagac gagcgtgtcg cccagcttga cgccgagcgt cttcgcaagg ccctcctcca
     4021 tgctcgcgcc atccggctcg cccgggatcc agcggccggc gacgatgcgg ttgtcgtccg
     4081 gcagctcgag ggtgtggctg aggttgaact cgcgctcgac catgcgcagc gcgttcgcgt
     4141 cgtgctcgta gcgcggcgcc gcgggttcgc cattgatcgt gagcaggcgg ccgcggatca
     4201 tcgggaacca gtcgtcgtgc cgcacgccgg cggccttgag cgccgcgcgg taggcgtcgc
     4261 cctggttggg ctggatgttg atcacgaagc ggtcgggcgc gcccggcggg gtcgccgcgc
     4321 gccagctgtc gatcaggtcg gtgcgcagca gcaccagcac ccacagcgcc aggagcccga
     4381 cggccatcga cgccacctgc agcaccgcga acgccggccg cgccgcgatc gcgcgcgtcg
     4441 cca
//
LOCUS       DASVLN010000558         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_243800, whole genome shotgun sequence.
ACCESSION   DASVLN010000558 DASVLN010000000
VERSION     DASVLN010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243800"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VF453_13450"
     CDS             <1..191
                     /locus_tag="VF453_13450"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX7688711.1"
                     /translation="DVLRDDGVAMAERLMAAGVDTVPVVYEGAPHSFLEAMETSAIAR
                     RGLDDGAAWLRARLFAPD"
     gene            263..1036
                     /locus_tag="VF453_13455"
     CDS             263..1036
                     /locus_tag="VF453_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688712.1"
                     /translation="MNDQIQAIQARLRQIDEYERKGLMPAAEAAAQRSAQQHKLMRLI
                     VPDAPAPRIPWRVHAWAVVAMLVLLGSVVAYLLTPLAGLRAPAIRMLQAVAAQNRKWH
                     EERLARERAGLSVAPDKYGRFPDELASAPAHPGAAASGPVAPLLAGRIVVSPALAGKF
                     SPEDSLFITVRLPDDPDGLPLAQLRVEAVNLPFNYDIAEAETLGGPQRLMTAPKVVVT
                     ARVSKSGSGKAQAGDLVGTSAPFAPWASHADVTIGQAVK"
     gene            complement(1086..>2076)
                     /locus_tag="VF453_13460"
     CDS             complement(1086..>2076)
                     /locus_tag="VF453_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HEX7688713.1"
                     /translation="MPAPGASPAARRYAAELGRGVNFGNMLDAPDEGAWGLRVDDRYV
                     ALVGPGGGGHIASVRLPVRWSNHAGADAQARIDPAFMARVDDVVHRLLARGVPVVVDM
                     HHYRQLDGDPLDPGEHAVPDDVVDVRFLAMWRQIAEHFRDAPPALAFELYNEPHGRLA
                     PRWNELMSRALGVVRRSNPGRIVVVGPTQWNSARGLGDFLMPPDANLVLTIHHYDPYP
                     FTHQGAPWVVPPMPTGVGCCSLQQLQDIRGPLDTAQRWGERHGYPVWVGEFGAFESVA
                     QPDRVRYTRTMRDEIERRGMTWAYWELAGGFGFIDPATADVRPNGIAAALFGP"
BASE COUNT          275 a          765 c          780 g          256 t
ORIGIN      
        1 gcgacgtgct gcgcgacgac ggcgtggcga tggccgagcg cctcatggcc gccggcgtcg
       61 acaccgtgcc ggtggtctac gagggcgcgc cccacagctt cctcgaggcg atggagacct
      121 cggcgatcgc gcgccgcggc ctggacgacg gcgccgcctg gctgcgggcg cggctgttcg
      181 ctccggactg agcgcccgcg ggggcgcccg gcgggcggtc gttcggcggg cctgcgaaaa
      241 tgggcggttg ccgacccgcc ccatgaacga ccagatccaa gccatccagg cgcgcctgcg
      301 ccagatcgac gagtacgagc gcaagggcct gatgcccgcc gccgaggccg ccgcgcagcg
      361 cagcgcgcag cagcacaagc tgatgcggct gatcgtgccc gacgcgcccg cgccgcgcat
      421 cccctggcgc gtgcacgcgt gggcggtggt ggcgatgctc gtgctgctgg gcagcgtcgt
      481 cgcctacctg ctcacgccgc tggcgggcct gcgcgcgccg gccatccgca tgctccaggc
      541 ggtcgcggcc cagaaccgca agtggcacga ggaacgcctc gcgcgcgagc gcgccggcct
      601 ctcggtcgcg cccgacaagt acggccgctt ccccgacgag ctcgcgtccg cgcctgccca
      661 cccgggcgcc gcggcctcgg gcccggtcgc cccgctgctc gccggccgca tcgtcgtgag
      721 cccggcgctg gcgggcaagt tctcgcccga ggactcgctg ttcatcaccg tgcgcctgcc
      781 cgacgacccc gacggcctgc cgctcgccca gctgcgcgtc gaggccgtca acctgccctt
      841 caactacgac atcgccgagg ccgagaccct cggcggcccg caacgcctga tgacggctcc
      901 caaggtcgtc gtgacggccc gcgtctcgaa gtccggcagc ggcaaggcgc aggcgggcga
      961 tctcgtcggc acgtcggcgc cgttcgcgcc gtgggcgagc catgcggacg tgacgatcgg
     1021 gcaggcggtg aagtgagggc ggattccgct cccgagcgag atcccgcctc cgcccgtccc
     1081 gcgcctcagg ggccgaacag cgccgcggct atcccgttgg gccgcacatc ggccgtggcg
     1141 gggtcgatga acccgaagcc gccggcgagc tcccagtacg cccaggtcat gccgcggcgc
     1201 tcgatctcgt cgcgcatcgt ccgcgtgtag cgaacgcggt cgggctgcgc gaccgactcg
     1261 aaggcaccga actcgccgac ccagacgggg tagccgtggc gctcgcccca acgttgcgcc
     1321 gtgtcgagcg ggccgcggat gtcctgcagt tgctgcaggg agcagcagcc gacgcccgtg
     1381 ggcatcggcg gcacgaccca gggcgcgccc tggtgagtga aggggtaggg atcgtagtgg
     1441 tggatcgtca gcacgaggtt cgcgtcgggc ggcatgagga agtcgccgag cccgcgggcg
     1501 gaattccact gcgtcgggcc gacgacgacg atgcgcccgg gattgctgcg ccgcacgacg
     1561 cccagcgcgc gcgacatgag ctcgttccag cgcggcgcga gccggccgtg cggctcgttg
     1621 tacagctcga acgcgagcgc gggcggcgca tcgcggaagt gctcggcgat ctggcgccac
     1681 atcgccagga agcgcacgtc gacgacgtcg tcgggcacgg cgtgctcgcc ggggtcgagc
     1741 ggatcgccgt cgagctggcg gtagtggtgc atgtccacga ccaccggcac gccgcgcgcg
     1801 agcaggcgat ggacgacgtc gtcgacacgc gccatgaacg cgggatcgat gcgggcctgg
     1861 gcatccgcgc cggcatggtt gctccagcgc accggcaggc ggacgctggc gatgtggccg
     1921 ccgccgcccg ggccgacgag ggcgacgtag cgatcgtcga cgcgcaggcc ccaggccccc
     1981 tcgtcgggcg catcgagcat gttgccgaag ttgacgccgc gcccgagctc ggcggcgtag
     2041 cggcgcgcgg ccggcgaggc gccgggcgcc ggcatc
//
LOCUS       DASVLN010000559         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_405189, whole genome shotgun sequence.
ACCESSION   DASVLN010000559 DASVLN010000000
VERSION     DASVLN010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405189"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="VF453_13465"
     CDS             complement(<1..1045)
                     /locus_tag="VF453_13465"
                     /inference="COORDINATES: protein motif:HMM:NF027763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapD/MoxY N-terminal periplasmic
                     domain-containing protein"
                     /protein_id="HEX7688714.1"
                     /translation="MSLIRQVWLLLALTLVLAFVGATGVSVRSARQYLQTQLDLKNND
                     TAQALALTLSQQRGDKVALELAIASQFDTGYYERIRLVAADGRAIVDRESDPRPHDAP
                     RWFVALMGLAPADGVAQVSDGWRQVGKLEVRSQSAFAEDELWHGTLRTVGVLAALMLF
                     AGLLAWVGVGRIRVPLARTVDQARALTERRFVMVDEPDVPELRNVTRAMNAMVARLKA
                     MFEEQAGQVEQLRRQAHCDPLTGLANRAHFLGRVKGMLDSEDGAAAGVLVMVRLVELQ
                     SLNRTLGRSATDRLLQDAAGAMVETSRRVAAAEVGRLNGSDFAIALPDVESARDPAVD
                     VAARLRSLLRVHDG"
     gene            complement(1113..1826)
                     /locus_tag="VF453_13470"
     CDS             complement(1113..1826)
                     /locus_tag="VF453_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEX7688715.1"
                     /translation="MILGAMARLRGRVLGALAEAVRLALAAAIVAMALAAPAVRGVDA
                     DRIQRSAAKYGPTAVANARALQQVMAEVAGKPDVVQLKAVNDFYNRRLAYREDIDNWG
                     VEDYWASPLEALGKGAGDCEDYAIGKYFTLVALGMPHSKLRMVYVRASIPDAPKGYVP
                     HMVLAYYPQADAEPLVLDNLLPEIHLAGQRPDLAPVFSFNAEGLWQGVGGIRASGDPL
                     VRLSKWREVLMRAQQDGFK"
     gene            2116..>3479
                     /locus_tag="VF453_13475"
     CDS             2116..>3479
                     /locus_tag="VF453_13475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX7688716.1"
                     /translation="MKKTVIAVSLLALASVAAMAQGTSPVAQSVQQAIEGNPEVAAKF
                     NAFRASNDEIDVARGAWRPHLDVSANAGKREYDLSQGSPKNPNFAEGGVRAELSQLLW
                     DGLATRNEIDRLGHAKLLRYFEFLDATEQFGMEAAKAYYDVVRYRKLVQLAEDNYVQH
                     KYSFDQVQSRVKAGVGRGVDLEQVLARVALAQSNLVTERANLHDVTERYVRIVGTLPP
                     AENASAVSMVDPLPASEADAMRQAALRSPAVAGAIENLRSARAAVAGRKSPFQPRIEA
                     RVRAAGGHNLDGQLYERRDATAEIALTWNVFNGGADSARERQYASLLNQAENERDKAC
                     NDVRQTAAIAFNDVAKLKEQLGYLDRNVVSIQKARDAYRQQFDIGQRSLIDLLNAENE
                     LYTAKRSYVLAEEDLATAVVRTYAAMGTLVANLGLKGPQAQDLAPEARDWGVGDDASS
                     RCPL"
BASE COUNT          552 a         1277 c         1182 g          468 t
ORIGIN      
        1 cgccgtcgtg cacgcgcagc aggctgcgca ggcgcgcggc gacgtcgacc gccggatcgc
       61 gcgcggactc gacgtcgggc agcgcgatcg cgaagtcgct gccgttcagg cgcccgacct
      121 ccgccgcggc gacgcggcgc gaggtctcga ccatcgcgcc cgcggcgtcc tgcagcaggc
      181 ggtcggtggc ggaccggccc agcgtgcggt tcagcgactg cagctccacg agacgcacca
      241 tcaccagcac gccggcggcc gcgccatcct cgctgtcgag catgcccttg acgcggccca
      301 ggaagtgcgc gcggttggcg aggccggtca gcgggtcgca gtgggcctgg cggcgcagtt
      361 gctcgacctg gccggcctgc tcctcgaaca tggccttcag gcgcgccacc atcgcgttca
      421 tcgcgcgcgt gacattgcgc agctccggca cgtcgggctc gtcgaccatc acgaagcggc
      481 gctcggtgag cgcgcgcgcc tggtcgacgg tgcgcgccag cggcacgcgg atgcggccga
      541 cgccgaccca cgcgagcagg cccgcgaaca gcatcagcgc cgccagcacg cccaccgtgc
      601 gcagcgtgcc gtgccagagc tcgtcctcgg cgaaggcgga ctggctgcgc acctccagct
      661 tgccgacctg gcgccagccg tcgctcacct gcgccacccc gtcggcgggc gcaagcccca
      721 tcagcgcgac gaaccagcgc ggcgcgtcgt ggggtcgcgg gtcggactcg cggtccacga
      781 tcgcgcggcc gtcggccgcg accagccgga tgcgctcgta gtagccggtg tcgaactggc
      841 tcgcgatcgc gagctccagc gccaccttgt cgccgcgctg ctgcgacagc gtcagcgcga
      901 gcgcctgcgc ggtgtcgttg ttcttgagat cgagctgcgt ctgcaggtac tggcgcgccg
      961 agcgcaccga gaccccggtc gcgcccacga acgcaagcac gagggtcaag gcgaggagca
     1021 accacacctg gcgaatcagc gacatgcact ctccattgcg ccgacctccg cgggtcggat
     1081 cttctcttgc ggacgcgggc ggcggccctc gttcacttga acccgtcctg ctgcgcgcgc
     1141 atcagcacct cgcgccattt cgacagccgc accagcggat cgccggacgc ccggatgcca
     1201 cctaccccct gccacagtcc ttccgcgttg aagctgaaca ccggggcgag gtcgggacgc
     1261 tggccggcca ggtggatctc cggcagcagg ttgtccagca ccagcggttc ggcatcggcc
     1321 tgcggataat aggccagcac catgtgcggc acgtagccct tcggtgcatc cgggatgctc
     1381 gcacgcacgt agaccatccg cagtttggaa tgcggcatcc cgagcgcgac gagcgtgaag
     1441 tatttgccga tggcgtagtc ctcgcagtcg ccggcgccct tgcccagcgc ctcgagcggg
     1501 ctcgcccaat agtcctcgac gccccagttg tcgatgtctt cccggtaggc gaggcggcgg
     1561 ttgtagaagt cgttgacggc cttgagctgg acgacgtcgg gcttgccggc gacctcggcc
     1621 atcacctgct gcagcgcgcg cgcgttggcc accgcggtcg ggccgtattt cgcggccgag
     1681 cgctggatgc ggtcggcgtc gaccccccgc acggccggcg ccgccaacgc catcgcgacg
     1741 atcgccgccg ccagggcgag ccgcaccgct tccgccagcg cgcccagcac gcgaccgcgc
     1801 aggcgcgcca tggccccgag gatcacggcg gcgtcgaggc aagggagaac gtcgacgagg
     1861 tccacatcgg acgtatcggc cgggcgcgcg cgatctggag cgcctccagc gtccgaaatg
     1921 catgccgacg ccccccgatt tccgcgcgcc gaccgcgtcg tgcccccgcc acagtggcgc
     1981 ggcacattcg gcggaaggcc cgaatgtttg ccggatcggc ctccagggac gccccggaac
     2041 gcccgggagc ggaggggggc ttcggtatca ttgaaacaac ttaatacaga tgcccccgag
     2101 acagcacctt ccatcatgaa gaagaccgtg attgcagtga gtctgctcgc gctggccagc
     2161 gtcgccgcca tggcgcaggg cacctcgccc gtggcccagt ccgtgcagca ggccatcgag
     2221 ggaaacccgg aagttgccgc caagttcaac gcatttcgcg cctccaacga cgagatcgac
     2281 gtcgcccgcg gcgcgtggcg gccgcacctg gacgtcagcg ccaatgccgg caagcgcgaa
     2341 tacgacctgt cgcagggatc gccgaagaac ccgaatttcg ccgaaggcgg ggtgcgcgcc
     2401 gagctgagcc agctgctgtg ggacggcctg gccacccgca acgagatcga ccgcctgggc
     2461 cacgccaagc tgctgcggta tttcgagttt ctcgacgcca ccgagcaatt cggcatggaa
     2521 gcggccaagg cgtattacga cgtcgtccgc taccgcaagc tcgtgcagct ggccgaggac
     2581 aactacgtcc agcacaaata ctcgttcgac caggtgcagt cgcgcgtgaa ggcgggcgtg
     2641 ggccgcggcg tcgatctcga gcaggtgctc gcccgcgtcg cgctcgcgca gtccaacctg
     2701 gtgaccgagc gcgccaacct gcacgacgtc accgagcgct acgtgcgcat cgtcgggacg
     2761 ctgccgccgg ccgagaacgc cagcgcggtg tcgatggtcg atccgctgcc ggccagcgag
     2821 gccgacgcga tgcgccaggc cgcgctgcgc agcccggccg tcgccggcgc gatcgagaac
     2881 ctgcgctccg cgcgcgccgc cgtcgccggg cgcaaaagcc cgttccagcc gcgcatcgag
     2941 gcgcgcgtgc gtgcggccgg cggccacaac ctcgacggcc agctctatga gcgccgcgat
     3001 gccaccgccg agatcgcgct cacctggaac gtcttcaacg gcggtgccga ctccgcacgc
     3061 gagcgccagt acgcgagcct gctcaaccag gccgagaacg agcgtgacaa ggcctgcaac
     3121 gacgtccgcc agacggcggc catcgcgttc aacgacgtcg ccaagctcaa ggagcagctg
     3181 ggctatctgg atcgcaacgt cgtctcgatc cagaaggcgc gcgacgccta ccgccagcag
     3241 ttcgacatcg gccagcgcag cctgatcgac ctgctcaacg cggaaaacga gctctacacc
     3301 gccaagcgct cctacgtgct ggccgaggag gacctggcca ccgcggtcgt gcgcacctac
     3361 gccgcgatgg gcacgctggt ggccaacctc ggcctgaagg ggccgcaggc gcaggatctc
     3421 gcgcccgagg cgcgggactg gggcgtcggc gacgacgcgt cctcgcgctg cccgctcga
//
LOCUS       DASVLN010000560        11259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_243946, whole genome shotgun sequence.
ACCESSION   DASVLN010000560 DASVLN010000000
VERSION     DASVLN010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243946"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2942
                     /locus_tag="VF453_13480"
     CDS             <1..2942
                     /locus_tag="VF453_13480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7688717.1"
                     /translation="IRSYAACPVAFEGLVVGTVCVLDPRPRALSPAQRASLGDVAGLV
                     EAILRTRCEQQLVHAERQHAIGLVRALAESEAMLSEAQRVARMGSWEIAPDGAISWSP
                     ALFEILGQDLARGPLSVEAFAARIHPEDVEAFEGGRVESLERQVPHERDFRFVLADGR
                     AIWLHAVSRPVRDAAGRVTGFRGTVQDVTERKDAERRAREAGERDRLLWQTTTDVVLF
                     LDEANRIQFCNPAVRHTFGHEPEDLLGRDLSVLQPLRKRAQHEAAFARYLRSGERRLD
                     WRTVETVAQHRDGREFPVEISFSDMVIDGHRVFGAFLRDVSARVGQQQALRLSEERYR
                     RIVQTAAEGIWTIDADSVTTFVNPTMAAMLGYAAEEMVGRPLFDFMREAEHAQAQANV
                     ERRRLGIAEQHDFRLRRKDGSDVWTAMSTTPAFDGEGRYAGALAMVTDITERRRAEDA
                     LRESEARFRSLTELSSDWYWEQDEEFRLTKVVGGRAFEKLAGFRRHLGQRPWEAPSDG
                     MAQAQWDAHRAQLEAHEPFRDFEITRRGTDGTLQIVSVSGEPQFGPDGVFRGYRGVGR
                     DVTEQRRAQVVRQELEAQLRESQKMEAIGVLAGGIAHDFNNVLAGILGNVALAAQDLA
                     PGHPAAPALEQIRKASLRGRELVQQILAFARRQPREITNCELRPLVEESLGLLRATLP
                     AGVTLESAIGPRPLHVLADATQVEQVLMNLCTNAWHALDGRPGRIAVTLDEAELSATE
                     ARRFAPPLAAGRYARLAVQDDGQGMDAATRARIFEPFFTTKEVGEGTGLGLAVAHGIV
                     AAHGGAIRVESAKGRGSLFEIFLPAIAPAPGPARPTPLATTHRGRGERVLYIDDDEVM
                     VTMVERLLERSGYAVACMHDPAAAIAAVRGAPGDWDIVVTDLNMPQLSGLDVARALRA
                     IRADLPVVISSGNLPDQLQADARQAGVRALLHKQYTLEELGAVIHWVLAGGQRLGLEP
                     LHQAGAG"
     gene            3067..3618
                     /locus_tag="VF453_13485"
     CDS             3067..3618
                     /locus_tag="VF453_13485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7688718.1"
                     /translation="MSTLRIAVVVGSLRRESFNRKLASALGKLMPPDVQLDRVEIGDL
                     PLFNQDDEQHPAAAVTRLKEQVKAADGVIFVTPEYNRSISGVLKNALDQGSRPYGQSV
                     WAKKPAGVIGISVGAIGTSLAQQHLRNVLAYLDMPTLGQPEVFLQHKDDTFQADGSLN
                     ESVAKFVGGWVSAYVAFVKRHQG"
     gene            complement(3697..7152)
                     /locus_tag="VF453_13490"
     CDS             complement(3697..7152)
                     /locus_tag="VF453_13490"
                     /inference="COORDINATES: protein motif:HMM:NF023280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,6-glucosidase domain-containing protein"
                     /protein_id="HEX7688719.1"
                     /translation="MQSPAAAAITEDASPAAANATTAQTLLHGLPAATAQADAADAPA
                     TTLRIHYHRPAGDYAGWQLHTWNAAVSPTWNAGWAPTGTDAFGDYWDVQLASGTGTVG
                     YLFHDGDTKDDGGADQAYVLQPGANEIWRVQGDLATYTSNPLTAPPPDIVTLRVHYQR
                     FAADYDRWGLHLWDGSGLDTSRLPGVNIGDWNHPVPFAAIPGEHAGTGEIVFDLPVLD
                     PATHPGSTSLEFIIHGLPPNDVNDKDGRPANIHVDFASLKIVNQVGEIWLVQQDPTVY
                     TAVPDLRSVSTTDARAVWLNGTLLQWPRVFAGNPVKLYWSMNGQIQAAAGQKITGADG
                     SIALDPYTGAVPTAAATRFKWVGAGGVFAVRASDVAKLKKLHTAQLLAVQEDATGAVQ
                     NATATQIAGAMDELYAAAQAATMGTTITHGTTTVRVWAPTAQAVTLYLYKTATGASTI
                     ATPLNLDPATGIWSLPIPGDFHGYYYAFGVRVFVRGVGVVRNIVTDPYSVSLAANSAR
                     SEFVDLDDPRSKPAGWDTSQPPATVAGSTDMSIYELHVRDFSDNDPTVPAAHRGKYLA
                     FTDASSNGMKHLRALAQAGLTDVHLMPAFDFSSVPETGCTTPSPAGAPDSSSQQAEVA
                     ATASTDCYNWGYDPWHFGAPEGSYATSVADGVTRVIEFRRMVQSLNAAGLRVGLDMVF
                     NHTAASGEDAHSVLDQIVPGYYHRLDATGAVTRDSCCDDTATENAMMGKLMSDTVAQW
                     ARQYHVSSFRFDIMGIQPRAAMMDVQAKVDAAVGRHVEMLGEGWNFGVVANNARFVQA
                     DMFDMNGTGIGTFNPYVRDAVRGGGCCDSGDALVANQGYVNGLWYDPNAQGGGHTAGD
                     LMWLGDVIKASLAGSVRGYPLETSWDATLPIEQIAFGGTPLGYVSQPTEVVNYVENHD
                     NLTLFDNDAYKLPTATSREDRARVQMLGAAIDAFSQGVAYFHAGVDTLRSKSLDANSY
                     DSGDWFNRLDWSYQADNFGVGLPPAASNQANWALAQPLLADPAIKPTATEIAWTRNVF
                     RDLLRIRASTTLLRLRTADDIKARLKFYNTGSRQLPTLLVGDVDGRGYPGANFDELAY
                     FINVDKQPHAITIPALQGRRFRLHPVQAAANATDKRAAAATVDDASGTFVIPARTAAV
                     FVVPQ"
     gene            7124..7405
                     /locus_tag="VF453_13495"
     CDS             7124..7405
                     /locus_tag="VF453_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688720.1"
                     /translation="MIAAAAGDCIAADPPPPPQAVIANAATTAAAACRAARIAAIQEV
                     FIALVSVHAQVQAARHAPPGPRLGAMLLKQNYITKLVLSLNELCNYICM"
     gene            complement(7460..7693)
                     /locus_tag="VF453_13500"
     CDS             complement(7460..7693)
                     /locus_tag="VF453_13500"
                     /inference="COORDINATES: protein motif:HMM:NF022397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEX7688721.1"
                     /translation="MDDQNILDRITALVEEERRLRADAAAPGERGDRLKQVAEQLDQC
                     WDLLRQRRAKREFGADPDSATPRDVGTVENYTQ"
     gene            8053..9495
                     /locus_tag="VF453_13505"
     CDS             8053..9495
                     /locus_tag="VF453_13505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7688722.1"
                     /translation="MHPPERRLDRLERLERVILAQSHLVQSDLDIDGFAQRVVEHLRE
                     LTESEGVTVELVDGEDTVCFHACGAVAPRPGRRLARAGSLSGLCVRTGAMVTCADTEA
                     DPRVDRQAARGVGARSMICTPLFRAGHAIGLVKVLYARPHAFDAGDVPVLSLMASTLA
                     GALGQQMAIESRSRVEARLRANEERMRSMLEHAHDAVVSMDDAGCVSQWNRAAERLFG
                     WAPIEAMGQPLAELIVPPARRGEFGRIVAGFAACERLEDVHQRVTVAAVDRAGRELAV
                     EVSLTATRVDGRWELTAFGRDVSERRHLEARLRELALSDGLTGLANRRAFMEALEKAV
                     ARAQRLGHRMALLFLDLDDFKQINDEHGHHAGDLALQAFARRIEQCVRKGDVVARLGG
                     DEFTVLAEGVESLEQAQAIAAKIAEAMQPPIEHGLRLHASIGVSLYRAPADASQFLRD
                     ADHAMYLAKRGRPPARVDEPDARRAEALFD"
     gene            9527..10027
                     /locus_tag="VF453_13510"
     CDS             9527..10027
                     /locus_tag="VF453_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7688723.1"
                     /translation="MSASHEHPAAGHDDASPPWKHDGVRVVPADKLDPNTAQTPGMDR
                     QAAITFARVGAQKLWAGTVHIHPNAKTGAHHHGPLESVIYVVRGRARMRWGERLEFTA
                     EAGPGDFIYVPPFVPHQEINASPTEVLECVLVRSDGQAVAVNLDIEPVEPPEDVAWVD
                     PTHPAP"
     gene            complement(10050..10565)
                     /locus_tag="VF453_13515"
     CDS             complement(10050..10565)
                     /locus_tag="VF453_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688724.1"
                     /translation="MTSTTARRVLATALGALALATAAVPAQAHGGYGYGYHGGYHGGY
                     HHGGYYHGGYYRGGWPFWAGVGLAVDVGLAGAYYYDRPYVVVDQPPVVYAPPAPVVVY
                     PNAAPAPAVAPAQPDPIFYPRNGQSPQQTEADRRACNAWATTQPSAMADASVFQRATL
                     ACMDGRGYTGR"
     gene            complement(10817..>11259)
                     /locus_tag="VF453_13520"
     CDS             complement(10817..>11259)
                     /locus_tag="VF453_13520"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEX7688725.1"
                     /translation="HVTRQQILREQLAGLRRQAAAAGSCAEAVPPPWASPPRVLCVGG
                     RARQLPVLQALVERRGGRFAHSAGDDAGIAALLPASDVVLLHAALLPAAAVRQVDAHC
                     RRAGTPCVRLDRLCARGFEQGLLEALARWTPTLGPAPASAPGDS"
BASE COUNT         1527 a         3942 c         4242 g         1548 t
ORIGIN      
        1 cgatccgctc ttacgcggcc tgcccggtcg ccttcgaggg gctggtcgtc ggcacggtgt
       61 gcgtcctcga cccgcggccg cgcgcgctct cgcccgcgca gcgcgcgagc ctgggcgacg
      121 tggcggggct ggtggaggcg atcctgcgca cgcgctgcga gcagcagctg gtgcacgcgg
      181 aacgccagca cgcgatcggc ctcgtgcggg cgctcgcgga gtcggaggcg atgctgtccg
      241 aggcgcagcg cgtcgcccgc atgggcagct gggagatcgc gccggacggc gccatctcgt
      301 ggtcgcccgc gctcttcgag atcctgggcc aggatctggc gcgggggccg ctgtcggtcg
      361 aggccttcgc ggcgcgcatc catcccgagg acgtcgaggc cttcgagggc ggccgcgtcg
      421 agtccttgga gcgccaggtg ccgcacgagc gcgacttccg cttcgtcctc gccgacggcc
      481 gcgccatctg gctgcacgcg gtgagccgcc cggtgcgcga cgccgcgggc cgcgtgaccg
      541 gcttccgcgg cacggtgcag gacgtcaccg agcgcaagga cgccgagcgc cgtgcgcgcg
      601 aggccgggga gcgcgaccgc ctgctgtggc agacgacgac ggacgtcgtg ctgttcctcg
      661 acgaggccaa ccgcatccag ttctgcaatc ccgcggtgcg ccacacgttc gggcacgagc
      721 cggaggatct cctgggccgc gatctctcgg tgctgcagcc gctgcgcaag cgtgcgcagc
      781 acgaggccgc gttcgcgcgc tacctgcgca gcggcgaacg gcgcctggac tggcgcaccg
      841 tcgagacggt ggcgcagcac cgcgacggcc gcgagttccc ggtcgagatc tcgttcagcg
      901 acatggtgat cgacggccac cgcgtcttcg gcgcgttcct gcgcgacgtc tcggcgcgcg
      961 tcggccagca gcaggcgctg cgcctgagcg aggagcgcta ccgccgcatc gtgcagaccg
     1021 cggccgaggg catctggacg atcgacgccg acagcgtcac gaccttcgtc aacccgacca
     1081 tggccgcgat gctgggctat gccgccgagg agatggtcgg ccgcccgctg ttcgatttca
     1141 tgcgggaggc cgagcacgcg caggcgcagg ccaacgtcga gcggcgccgc ctgggcatcg
     1201 cggagcagca cgacttccgg ctgcgccgca aggacggcag cgacgtgtgg acggcgatga
     1261 gcacgacgcc cgccttcgac ggcgaaggcc gctacgccgg cgcgctggcg atggtcaccg
     1321 acatcaccga gcgccgccgc gccgaggacg cgctgcgcga gagcgaggcg cgcttccgct
     1381 cgctgaccga gctgtcgtcg gactggtact gggagcagga cgaggaattc cgcctcacca
     1441 aggtcgtggg cgggcgcgcg ttcgagaagc tggccggctt ccggcgccac ctcggccagc
     1501 ggccgtggga ggcgcccagc gacggcatgg cgcaggcgca gtgggacgcg caccgcgcgc
     1561 agctggaggc gcacgagccg ttccgcgact tcgagatcac gcgccgcggc acggacggga
     1621 cgctgcagat cgtcagcgtc agcggcgagc cccagttcgg ccccgacggc gtcttccggg
     1681 gctaccgcgg cgtgggccgc gacgtcacgg agcagcgtcg cgcgcaggtg gtgcgccagg
     1741 agctcgaggc ccagctgcgc gaatcgcaga agatggaggc gatcggcgtg ctggccggcg
     1801 gcatcgcgca cgacttcaac aacgtgctgg cgggcatcct cggcaacgtg gcgctcgcgg
     1861 cgcaggacct ggcgcccggc catcccgcgg cgccggcgct ggagcagatc cgcaaggcga
     1921 gcctgcgcgg gcgcgagctg gtgcagcaga tcctcgcctt cgcgcgccgc cagccgcgcg
     1981 agatcaccaa ctgcgagctg cggcccctgg tcgaggagag cctgggcctg ctgcgcgcca
     2041 cgctgccggc gggcgtgacg ctggagtcgg cgatcggccc gcggccgctg cacgtgctcg
     2101 cggacgcgac gcaggtcgag caggtgctga tgaacctgtg caccaacgcc tggcatgcgc
     2161 tggacggccg gcccggccgc atcgcggtga cgctggacga ggccgagctc tccgccaccg
     2221 aggcgcgccg cttcgcgccg ccgctggccg cgggtcgcta cgcccggctg gcagtgcagg
     2281 acgacgggca gggcatggac gcggcgacgc gggcgcgcat cttcgagccc ttcttcacga
     2341 ccaaggaagt gggcgagggc accgggctcg gactggcggt ggcgcacggc atcgtcgccg
     2401 cccacggcgg ggcgatccgg gtcgagtcgg ccaagggccg cggcagcctg ttcgagatct
     2461 ttctgcccgc catcgcgccc gcgcccggac ccgcgcgccc gacgccgctg gccacgacgc
     2521 atcgcggccg cggcgagcgc gtgctctaca tcgacgacga cgaggtgatg gtcacgatgg
     2581 tcgagcgcct gctcgagcgc agcgggtacg ccgtcgcgtg catgcatgac cccgcggccg
     2641 cgatcgccgc cgtgcgcggc gcccccgggg actgggacat cgtcgtgacc gacctcaaca
     2701 tgcctcagct ctcgggcctg gacgtggcga gggcgctgcg cgccatccgc gcggacctgc
     2761 cggtcgtgat cagctcgggc aacctgcccg accagttgca ggccgacgcg cgccaggccg
     2821 gcgtgcgcgc gctgctgcac aagcagtaca cgctcgagga gctgggcgcc gtcatccact
     2881 gggtgctcgc cggcggccag cggctcgggc tggagccgct gcaccaggcc ggcgcgggct
     2941 gacgcgccgc tgccgcggtc gggcgatgcc tgacatcgcg gcggggcgcg gagctcgcgg
     3001 gcgcccgcga aggccggtgg acggccacaa tgccgtcgtc catcaccgaa acccgaagga
     3061 gcccgcatga gcaccctgcg catcgccgtc gtcgtcggca gtctccgccg cgagtcgttc
     3121 aaccgcaagc tggccagcgc gctcggcaag ctgatgccgc cggacgtcca gctcgaccgc
     3181 gtcgagatcg gcgacctgcc gctgttcaac caggacgacg agcagcatcc cgccgcggcc
     3241 gtcacgcgac tgaaggagca ggtcaaggcg gccgacggcg tgatcttcgt gacgccggag
     3301 tacaaccgct cgatctccgg cgtcctcaag aacgcgctcg accagggctc gcggccgtac
     3361 ggccagagcg tctgggcgaa gaagccggcg ggcgtgatcg gcatctcggt cggcgcgatc
     3421 ggcacctcgc tcgcgcagca gcacctgcgc aacgtgctcg cctacctcga catgcccacg
     3481 ctggggcagc ccgaagtctt cctgcagcac aaggacgaca ccttccaggc ggacggcagc
     3541 ctcaacgaaa gcgtcgcgaa gttcgtgggc gggtgggtct ccgcgtacgt cgccttcgtg
     3601 aagcgccacc agggctgaag gacgcggcgg acgcacgccg cccgacaagg gacggcgcca
     3661 cgaccgccgc gcgcacgggc tcggcgtccc gcccgatcac tgcgggacga cgaagaccgc
     3721 cgcggtgcgc gcggggatca cgaaggtgcc gctcgcgtcg tcgaccgtcg ccgccgcggc
     3781 gcgcttgtcg gtcgcgttgg cggcggcctg caccggatgg agccggaagc ggcggccctg
     3841 cagcgccggg atcgtgatcg cgtgcggctg cttgtcgacg ttgatgaagt aggcgagctc
     3901 gtcgaagttg gcgccggggt agccgcggcc gtcgacatcg ccgaccagca gcgtcgggag
     3961 ctgccgcgag cccgtgttgt agaacttcag gcgcgccttg atgtcgtcgg cggtgcgcag
     4021 gcgcagcagg gtcgtgctcg cgcggatgcg cagcaggtcg cggaagacgt tgcgggtcca
     4081 cgcgatctcg gtggcggtgg gcttgatcgc gggatccgcg agcagcggct gcgcgagcgc
     4141 ccagttggcc tggttcgagg cggccggcgg caggccgacg ccgaagttgt cggcctggta
     4201 gctccagtcg aggcggttga accagtcgcc cgagtcgtag ctgttggcgt ccagcgactt
     4261 gctgcgcagc gtgtcgacgc cggcgtggaa gtaggccacg ccctgactga acgcgtcgat
     4321 cgcggcgccc agcatctgca cccgcgcgcg gtcctcgcgc gaggtcgccg tgggcagctt
     4381 gtaggcgtcg ttgtcgaaca gcgtcaggtt gtcgtggttc tccacgtagt tgacgacctc
     4441 cgtcggctgc gagacgtagc cgagcggcgt gcccccgaag gcgatctgct cgatcggcag
     4501 ggtcgcgtcc cagctcgtct cgagcgggta gccgcgcacc gagcccgcga ggctcgcctt
     4561 gatgacgtcg cccagccaca tgaggtcgcc cgccgtgtgg ccgccgccct gggcgttcgg
     4621 gtcgtaccac aggccgttga catagccctg gttggcgacc agcgcgtcgc ccgagtcgca
     4681 gcagccgccg ccgcgcacgg cgtcgcgcac gtaggggttg aaggtgccga tgcccgtgcc
     4741 gttcatgtcg aacatgtcgg cctgcacgaa gcgcgcgttg ttggcgacga cgccgaagtt
     4801 ccagccttcg ccgagcatct cgacatggcg gcccacggcg gcgtcgacct tggcctgcac
     4861 gtccatcatc gcggcgcgcg gctggatgcc catgatgtcg aaccggaacg agctcacgtg
     4921 gtattgcctc gcccactggg cgacggtgtc gctcatgagc ttgcccatca tcgcgttctc
     4981 ggtcgccgtg tcgtcgcagc acgagtctcg cgtgacggcg ccggtcgcgt cgagccggtg
     5041 gtagtagccg gggacgatct ggtcgagcac cgagtgcgcg tcctcgcccg aggccgccgt
     5101 gtggttgaac accatgtcca ggccgacgcg caggcccgcg gcgttgagcg actgcaccat
     5161 gcggcggaac tcgatgacgc gggtgacgcc gtctgcgacg gaggtggcgt agctgccctc
     5221 gggcgcgccg aagtgccagg ggtcgtagcc ccagttgtag cagtcggtcg aggcggtcgc
     5281 ggcgacctcg gcctgttgcg agctcgagtc cggcgctccg gcgggcgagg gcgtcgtgca
     5341 gccggtctcc gggacgctcg agaagtcgaa cgccggcatc aggtgcacgt cggtcaggcc
     5401 ggcctgcgcg agcgccctca ggtgcttcat gccgttggag ctggcgtcgg tgaaggcgag
     5461 gtacttgccc cggtgcgcgg ccggcaccgt ggggtcgttg tccgagaagt cgcgcacgtg
     5521 cagctcgtag atcgacatgt cggtggagcc cgcgaccgtc gcgggcggct gcgaggtgtc
     5581 ccagccggcg ggcttggagc gcgggtcgtc gaggtcgacg aactcgctgc gggcgctgtt
     5641 ggccgccagg ctcaccgagt acgggtcggt gacgatgttg cgcacgacgc cgacgccgcg
     5701 cacgaacacg cgcacgccga aggcgtagta gtagccgtgg aagtcgccgg ggatcgggag
     5761 cgaccagatg cccgttgccg ggtcgaggtt caggggcgtg gcgatcgtgc tcgcaccggt
     5821 cgccgtcttg tagagataca gcgtgacggc ctgcgcggtc ggggcccaga cgcgcaccgt
     5881 cgtcgtgccg tgcgtgatcg tggtgcccat cgttgccgcc tgcgcggcgg cgtagagctc
     5941 gtccatcgcg cccgcgatct gcgtggcggt cgcgttctgc acggcgccgg tcgcgtcctc
     6001 ctgcaccgcg agcagctgcg cggtgtgcag cttcttcagc ttcgcgacgt ccgacgcgcg
     6061 tacggcgaac acgccgcccg cgccgaccca cttgaagcgc gtcgccgcgg cggtcggcac
     6121 ggcgccggtg tacggatcga gcgcgatcga gccgtcggcg ccggtgatct tctgtccggc
     6181 ggcggcctgg atctgcccgt tcatcgacca gtacagcttg accgggttgc cggcgaacac
     6241 gcgcggccac tgcagcagcg tgccgttcag ccagacggcg cgcgcgtctg tggtggagac
     6301 ggagcgcagg tcggggacgg cggtgtagac ggtcggatcc tgctgcacga gccagatctc
     6361 gccgacctgg ttgacgatct tcaggctcgc gaagtcgacg tggatgttgg cggggcggcc
     6421 gtccttgtcg ttgacgtcgt tgggcggcag gccgtggatg atgaactcga gcgaggtcga
     6481 gcccggatgc gtggccggat cgagcaccgg caggtcgaac acgatctcgc ccgtgcccgc
     6541 gtgctcgccg gggatggcgg cgaacggcac cgggtggttc cagtcgccga tgttcacgcc
     6601 cggcaggcgc gacgtgtcca gcccgctgcc gtcccacagg tgcaggcccc agcggtcgta
     6661 gtcggcggcg aagcgctggt agtgcacgcg caaggtgacg atatccggcg gcggggccgt
     6721 cagcgggttg ctcgtgtagg tcgcgaggtc gccctggacg cgccagatct cgttggcgcc
     6781 cggctgcagc acgtaggcct ggtcggcgcc gccgtcgtcc ttggtgtcgc cgtcgtggaa
     6841 caggtagccg accgtgccgg tgccggaggc gagctgcacg tcccagtagt cgccgaacgc
     6901 gtcggtgccg gtgggcgccc agccggcgtt ccaggtcggc gagaccgcgg cgttccaggt
     6961 atggagctgc cagcccgcgt agtcgccggc ggggcggtgg tagtggatgc gcagcgtcgt
     7021 ggcgggcgcg tcggcggcgt ccgcctgcgc ggtggcggcg ggcaggccgt gcagcagggt
     7081 ctgggccgtc gtcgcgttcg ccgccgccgg cgaggcgtcc tccgtgatcg cggcggcggc
     7141 gggcgactgc atcgccgccg atccgccgcc gccgccgcag gcggtcatcg ccaatgcggc
     7201 gaccacggcc gcggccgcat gccgtgccgc gcgcatcgcg gccatccagg aagtcttcat
     7261 cgcgcttgtc tccgtgcacg cgcaggtcca ggcggcgcga catgctccgc ctggcccgcg
     7321 tctcggcgcg atgctattga agcaaaacta catcaccaag ctcgtacttt ccctgaacga
     7381 gttgtgtaac tacatttgca tgtaatctca aacatcgggg aacgacgcca tgatcggcag
     7441 aggcgagcct cgccggacgt cattgcgtgt agttctccac cgtgcccacg tcgcgaggcg
     7501 tggccgaatc cgggtcggcg ccgaactcgc gcttcgcgcg gcgctggcgc agaagatccc
     7561 agcattggtc cagctgctcc gccacctgct tgaggcggtc gccgcgctcg ccaggcgctg
     7621 ccgcgtcggc acgcagccgg cgttcttcct ccacgagggc ggtgatgcga tcgaggatgt
     7681 tctggtcgtc catgtccgag gctcctgggt ggtgatcgag gcgcgcgggc aatccgcgaa
     7741 cccacggcct gcggcctcgg cggacgacgc cctgcggcgt gcggcgcagc gcgccttcgg
     7801 acccgagctc gcgccgcgcg cgatccaggg ttttcacggg cccccgcccc gagcgcgggc
     7861 gacgagcgga ttggacaacg cgaccgcgcg cccgcatact gccagttggg attcgaacac
     7921 ggaaactgtc cgccgcgcgg cttgggggcc gcgtgactgg gagctccggc gcgcgtcatc
     7981 gcggggtcac accgcgagcg caggcgccgc ggcatgcgag ccagctcgag attcgacacc
     8041 cgggggtatc gcatgcatcc acctgagcgt cggctcgacc gcctcgaacg actcgagcgc
     8101 gtgatcctcg cgcagtcgca tctcgtccag tccgacctcg acatcgacgg cttcgcgcag
     8161 cgggtcgtgg agcacttgcg cgagctcacc gagtccgaag gcgtcacggt ggagctcgtg
     8221 gacggggagg acaccgtctg cttccacgcc tgcggcgcgg tggcaccgcg gcccggccgg
     8281 cggctcgcgc gcgcggggag cctgtcgggg ctgtgcgtgc gcaccggcgc gatggtcacc
     8341 tgcgcggaca ccgaggccga cccgcgcgtc gaccgccagg ccgcgcgcgg cgtgggtgcg
     8401 cgttccatga tctgcacgcc gctgttccgc gccgggcacg cgatcggcct ggtcaaggtg
     8461 ctctacgcgc gcccgcacgc cttcgacgcc ggcgacgtgc cggtgctgtc gctgatggcg
     8521 tcgacgctgg ccggggcgct cgggcagcag atggccatcg agtcgcgcag ccgcgtcgag
     8581 gcgcgcctgc gcgccaacga ggagcgcatg cgatcgatgc tggagcatgc gcacgacgcc
     8641 gtggtctcga tggacgacgc gggctgcgtg tcgcagtgga acagggccgc ggagcgcctg
     8701 ttcggctggg cgccgatcga ggcgatgggc cagccgctcg ccgagctcat cgtgccgccc
     8761 gcccggcgtg gcgagttcgg tcgcatcgtc gcgggcttcg cggcctgcga gcggctcgag
     8821 gacgtccacc agcgcgtcac ggtggccgcg gtcgaccgcg ccggccgcga gctggccgtc
     8881 gaggtgagcc tcacggccac gcgcgtggac ggccgctggg agctcaccgc cttcggccgc
     8941 gacgtctccg agcgccggca tctggaggcc cgcctgcgcg agctcgcact gagcgacggc
     9001 ctcaccggcc tggccaaccg gcgcgcgttc atggaggcgc tcgagaaggc cgtcgcgcgg
     9061 gcccagcggc tggggcaccg catggcgctg ctgttcctcg acctcgacga cttcaagcag
     9121 atcaacgacg agcacggcca ccacgccggc gacctcgcgt tgcaggcctt cgcgcgccgc
     9181 atcgagcagt gcgtgaggaa gggcgacgtc gttgcgcgcc tgggcggcga cgagttcacc
     9241 gtgctggccg agggcgtcga atcgctcgag caggcccagg cgatcgccgc caagatcgcc
     9301 gaggcgatgc agccgccgat cgagcacggc ctgcgcctgc acgccagcat tggcgtgagc
     9361 ctgtaccgcg cgcccgcgga cgcctcgcag ttcctgcgcg acgccgacca cgcgatgtac
     9421 ctcgccaagc gtggccgccc gccggcccga gtggacgagc cggacgcgcg gcgcgcggag
     9481 gcgctgttcg attgacaatg cgcggatcgc ccctgaggag atcgccatgt ccgcatcgca
     9541 cgaacatccc gccgcaggcc acgacgacgc gtcgccgccg tggaagcatg acggcgtgcg
     9601 cgtcgtcccg gccgacaagc tcgatcccaa caccgcgcag acgcccggca tggatcgcca
     9661 ggccgcgatc acctttgccc gcgtcggcgc ccagaagctg tgggccggca cggtgcacat
     9721 ccatcccaac gccaagaccg gcgcacacca ccacgggccg ctcgagagcg tgatctacgt
     9781 cgtgcgcggg cgcgcccgca tgcgctgggg cgagcgcctg gagttcaccg ccgaggcggg
     9841 cccgggcgac ttcatctacg tgccgccgtt cgtgccgcac caggagatca acgcgagccc
     9901 gacggaggtg ctcgagtgcg tgctggtgcg cagcgacggg caggcggtcg cggtcaacct
     9961 cgacatcgag ccggtcgagc cgccggagga cgtggcctgg gtcgacccga cgcatccggc
    10021 gccgtaggcg aggccggtcg cggggcgggt cagcggccgg tgtagccccg cccgtccatg
    10081 caggcgagcg tcgcgcgctg gaagacgctc gcgtcggcca ttgcgctggg ttgggtcgtc
    10141 gcccacgcgt tgcaggcgcg acggtcggcc tcggtctgct gcggggactg gccgttgcgc
    10201 ggatagaaga tcggatcggg ctgcgccggc gcgacggccg gggcgggggc cgcgttcggg
    10261 tacaccacga cgggcgcggg cggagcgtag accaccggtg gctggtcgac gacgacgtag
    10321 ggccggtcgt agtagtaggc gccggcgagg ccgacgtcca ccgcgaggcc gacgccggcc
    10381 cagaacggcc acccgccgcg gtagtagccg ccgtggtaat acccgccgtg gtggtagcca
    10441 ccgtgatagc ccccgtggta gccgtatccg tagccgccgt gcgcctgcgc ggggacggcg
    10501 gccgtggcca gggccagcgc gccgagggcg gtcgcgagga cgcggcgggc ggtggtggat
    10561 gtcatgtcga actccttcgc ggcgcgccgg gcgccaatga accggatgcc gggcccggcg
    10621 gcgaaccctg tcgagctcac gggcgcgcgc gcggccaagc gaagtcccgc gggacatccg
    10681 tgttcgaccg cacgcccgcg attttgttcc ggcgcgcggc cgacggcacg aaaaaagacg
    10741 ccacccgccg ggacgccggc gaatggccaa tcctgccgcc gcccgggcgc gtccgctgga
    10801 cagtgggcgg cgacagtcag gaatctccag gcgccgatgc aggggccggg ccaagcgtcg
    10861 gcgtccagcg cgcgagcgcc tcgagcagcc cctgctcgaa cccgcgggcg cacaggcgat
    10921 ccaggcgtac gcacggcgtc cccgcgcgac ggcagtgggc gtcgacctgg cgaacggcgg
    10981 cggccggcag cagcgcggca tgcagcagta cgacgtcgga cgccggcagc agcgcggcga
    11041 tgcccgcgtc gtcgcccgcc gagtgggcga agcggccgcc gcggcgttcg accagggcct
    11101 gcagcacggg cagctggcgg gcgcggccgc cgacgcacag cacccgcggt ggcgacgccc
    11161 agggcggcgg gacggcttcc gcgcaggacc cggcggccgc tgcctgccgg cgcagcccgg
    11221 cgagctgctc gcgcaggatc tgctgtcgtg tcacgtgca
//
LOCUS       DASVLN010000561         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_29630, whole genome shotgun sequence.
ACCESSION   DASVLN010000561 DASVLN010000000
VERSION     DASVLN010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29630"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VF453_13525"
     CDS             complement(<1..1680)
                     /locus_tag="VF453_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7688726.1"
                     /translation="MALLTLTNAHLAFGHVALLDGTDFALEAAERVALIGRNGTGKSS
                     LLKILAGLDKPDDGLLQVQQGLRRTYVPQEPQFAPGVSVFEAVSDGVAEAKAARERFE
                     RHEDLDHDALDALQSRIEALGGWDWERRVDETLQRLDLAPELKVDTLSGGVRKRVALA
                     QALVAKPDVLLLDEPTNHLDLDAIDWLAELLRGYRGALVLISHDRAFIDATATRIVEL
                     DRGQLRSYPGNFSAYESAKARELEAEALANARADKLLAQEEVWIRKGVEARRTRSVAR
                     IARLEQLRADRAARRDQVGRVRMEVATGGQSGKVVAELEDVSMRFGERVIVDKLTTTI
                     LRGDKIGLIGPNGCGKTTLLKLILGQLEPTAGRIKRGTQITVAYFDQMRAGLKLDATL
                     ADTISPGSEWVEIGSTRKHIMSYLGDFLFAPERANAKVVSLSGGERNRLLLARLFALP
                     ANVLVLDEPTNDLDIDTLELLEELLASYPGTVFLVSHDRRFVDNVVTSTLVWEGDTSP
                     GRWREYEGNVEDWQAQRARVRASAPAPTPPSATAPAPVSLSVSAAAAPAALP"
     gene            complement(1680..2663)
                     /gene="gshB"
                     /locus_tag="VF453_13530"
     CDS             complement(1680..2663)
                     /gene="gshB"
                     /locus_tag="VF453_13530"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX7688727.1"
                     /translation="MKLLFVADPLESFKIYKDSTFVMMREAQARGHEIWACEPVDLRW
                     QLGARVEAHARPIVLNGVSGRSHAPWFETEGARVAAVADFDAVLMRKDPPFDSEFFYA
                     TQLLSHAEREGAIVLNSPRALRDHPEKLAILEFPQFIAPTLVTRSEADVRAFHAQHRD
                     VILKPLDGMGGMGIFRVRDDGLNLGSIVETLNRGGAQTIMVQKFLPDIVDGDKRVLVI
                     AGEPVPHTLARIPQGGEVRGNLAAGGLGVARDITPREREIAAVIGPKLAARGLLLIGL
                     DVIGDTLTEINVTSPTCFQEITDQTGYDVARTFVDALEKTVKFHLDLKKKN"
BASE COUNT          370 a          968 c         1027 g          429 t
ORIGIN      
        1 cggcaacgcc gcgggcgcgg ccgccgccga caccgacagc gacaccggcg ccggcgcggt
       61 cgcggagggt ggcgtcggcg ccggggcgct tgcgcgcacg cgggcacgct gggcctgcca
      121 gtcctcgacg ttgccttcgt attcgcgcca gcggccgggg ctcgtgtcgc cttcccagac
      181 cagcgtgctc gtgaccacgt tgtcgacgaa gcggcggtcg tggctcacga ggaagacggt
      241 gccggggtag ctggccagca gctcctcgag cagctccagc gtgtcgatgt cgaggtcgtt
      301 ggtcggttcg tcgagcacca gcacgttggc cggcagcgcg aacaggcgcg cgagcagcag
      361 ccggttgcgc tcgccgcccg acagcgagac caccttcgcg ttcgcacgct ccggcgcgaa
      421 caggaagtcg ccgaggtagc tcatgatgtg cttgcgcgtc gagccgatct cgacccactc
      481 gctgccgggg ctgatcgtgt cggcgagcgt cgcgtccagc ttcaggccgg cgcgcatctg
      541 gtcgaagtag gccaccgtga tctgcgtgcc gcgcttgatc cggccggcgg tgggctcgag
      601 ctggccgagg atcagcttca gcagcgtcgt cttgccgcag ccgttggggc cgatcaggcc
      661 gatcttgtcg ccgcgcagga tcgtggtcgt gagcttgtcg acgatcacgc gctcgccgaa
      721 gcgcatcgag acgtcctcga gctcggcgac gaccttgccc gactgcccgc cggtggcgac
      781 ttccatgcgc acacgcccga cctggtcgcg ccgcgcggcg cggtcggcgc gcagctgctc
      841 cagccgcgcg atgcgcgcga cgctgcgcgt gcggcgcgcc tcgacgccct tgcggatcca
      901 gacctcctcc tgcgcgagca gcttgtccgc gcgcgcgttg gcgagcgcct ccgcctcgag
      961 ctcgcgcgcc ttggcggact cgtacgcgct gaagttgccg ggatagctgc gcagctggcc
     1021 gcggtcgagc tccacgatgc gcgtggccgt ggcgtcgatg aaggcgcggt cgtgcgagat
     1081 cagcacgagc gcgccgcgat agccgcgcag gagctcggcg agccagtcga tcgcgtcgag
     1141 gtcgaggtgg ttggtcggct cgtcgagcag cagcacgtcg ggcttggcga ccagcgcctg
     1201 ggcgagcgcc acgcgcttgc gcacgccgcc cgacaaggtg tcgaccttca gctccggcgc
     1261 gaggtccagc cgctgcagcg tctcgtcgac gcggcgctcc cagtcccagc cgcccagcgc
     1321 ctcgatgcgc gactgcagcg cgtcgagcgc gtcgtggtcg agatcctcgt ggcgctcgaa
     1381 gcgctcgcgc gcggccttgg cctcggccac cccgtcgctc accgcctcga agacgctcac
     1441 gcccggcgcg aactggggct cctgcggcac gtaggtgcgg cgcagtccct gctgcacctg
     1501 gagcaggccg tcgtcgggct tgtccaggcc ggcgaggatc ttcagcaggc tcgacttgcc
     1561 ggtgccgttg cgcccgatca gcgcgacgcg ctccgcggcc tcgagcgcga agtcggtgcc
     1621 atccagcagg gcgacgtgac cgaacgccag gtgcgcgttc gtcagggtga gcagggccat
     1681 cagttcttct tcttcaggtc gaggtggaac ttcaccgtct tctccagcgc gtcgacgaag
     1741 gtcctggcga cgtcgtagcc ggtctggtcg gtgatttcct ggaagcaggt cgggctggtg
     1801 acgttgatct ccgtgagcgt gtcgccgatc acgtccaggc cgatcagcag caggccgcgc
     1861 gcggcgagct tcgggccgat caccgccgcg atctcgcgct cgcgcggcgt gatgtcgcgc
     1921 gccacgccca ggccgccggc ggcgaggttg ccgcgcacct cgccgccttg cgggatacgg
     1981 gccagggtat gcggcacggg ctcgccggcg atcacgagca cgcgcttgtc gccgtcgacg
     2041 atgtcgggca ggaacttctg caccatgatg gtctgcgccc cgccgcggtt gagcgtctcc
     2101 acgatgctgc ccaggttcag cccgtcgtcg cggacgcgga agatgcccat gccgcccatg
     2161 ccgtccagcg gcttgaggat gacgtcgcgg tgctgggcat ggaaggcgcg cacgtccgcc
     2221 tcggaacgcg tgaccagcgt cggcgcgatg aactgcggga actcgaggat cgcgagcttc
     2281 tcgggatggt cgcgcagtgc gcgcggcgag ttcagcacga tcgcgccctc gcgctcggcg
     2341 tggctcagga gctgagtcgc atagaagaac tcgctgtcga agggcggatc cttgcgcatc
     2401 agcacggcgt cgaaatcggc caccgcggcg acgcgggcgc cctcggtctc gaaccagggc
     2461 gcgtggctgc ggccgctcac cccgttgagc acgatgggcc gggcgtgcgc ctcgacgcgg
     2521 gcgccgagct gccagcgcag gtcgacgggc tcgcaggccc agatctcgtg gccgcgggcc
     2581 tgggcctcgc gcatcatcac gaaggtggag tccttgtaga tcttgaagga ctcgagcggg
     2641 tcggcgacga acagcagttt catgggaggc ccctggcagg aacgggaggc gcggccggcg
     2701 cgcgcgccgc ggccgacatc atggcgcgaa gcgcgcggac gcgggcggac gggttctttg
     2761 cgccggcgcc ggtggcgcgc cagtgttgca cagc
//
LOCUS       DASVLN010000562         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_83871, whole genome shotgun sequence.
ACCESSION   DASVLN010000562 DASVLN010000000
VERSION     DASVLN010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83871"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..>3702)
                     /locus_tag="VF453_13535"
     CDS             complement(58..>3702)
                     /locus_tag="VF453_13535"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7688728.1"
                     /translation="AAVLTRRELRLRAALADDLREGRNRLELSERRLKAITDNLPALI
                     GYVDRHEAYRFLNAAYRPIFGVDPDATVGRTMAEVLGPATYARVEEHVRAALAGQTRT
                     FEHHGIGRAADIVLETSYLPDINPTGGVEGFYVMAVDVTARKAAELELARGERRLRMV
                     TDSVPALIGHVDANQVYRFVNATYEAVFGIPQSEFIGRTLEQMLGVAAYAKVAAPVAA
                     ALRGEPQRFELVGIGRRPDATFDVNLLPDFNPAGCVDGFYVMTMDITERKRAETALAT
                     GERRLRTITDNLPATVTRFDRSLTCTYANERFREFYGVDPASLVGSSQRAFRGEAEWE
                     VLAPHVEAVLRGERRDFEAVAVMRGEPHWMQQSLIPEHDAAGGVTGYLSVAFDITERK
                     RQEEALRKSELFLDRSGQLGGIGGWELDVESMALVWSDETKRIHGVPPGYAPSFAAAL
                     DFYPAPYQARVIGLVQQAITDGTPYDVEMPLVRADGRRITVHVVAAVERVQGEPARLV
                     GAIQDVTELVAQREALTEANERIALATESGGIGIWELDLDTGELLWDKRMHALYGDAA
                     DGRASPVTLWDQRMHPDDIAGIDLAIREAAAGGAPLDREFRLVMADGSTRHLRGTARV
                     THDASGRPRRLIGATWDITELRQLAADLAEDRQLLRVTLESIGDAVITTDAHGMITWL
                     NPVAERLTGWDSAEARDRPLGQVFRVVDERTREPAPDPVAGCLAPGRTERNVAPSLLV
                     SRAGREYGIEDSAAPIRDEAGEVLGVVIVFHDVSEQRRLSGEMNWRATHDALTGLVNR
                     AEFEARLKRVLDKSHEDASEHALLYIDLDQFKLVNDACGHSVGDVLLQQMARMLADAV
                     RARDTVARLGGDEFAIIMEHCGAERALHTAQRLCERMEDFRFIHEERRFRIGTSIGLV
                     PVDRRWTSVAAIQQAADSSCYAAKEAGRNRVHVWMDSDAALRERQFEMQWTARIEQAL
                     DEDGFALFAQRIEPLKHATAGVHAEVLLRMKQADGSLVAPGAFLPAAERFHLASRIDR
                     WVLRRVVDWMIALREPGLIELLSVNLSGQSVGDRTFHRWALELLGRAGPRVCGRLCLE
                     ITETAAVTRMADAAAFVDEVRAIGVQVALDDFGAGAASFGYLKTLRVDTLKIDGQFVR
                     DLVDDALDEAAVRCFADVAKVMGLRTVAEFVDKPSVLERLRALDVDFAQGFLLHRPAP
                     IGELVAPPGA"
BASE COUNT          479 a         1347 c         1354 g          522 t
ORIGIN      
        1 gtgcagtatc gcgtcccggc cgcgcggctg ctcgccccgg ctgggggcgg cgcgggtcta
       61 cgcccccggc ggcgcgacca gctcgccgat cggcgccggc cggtgcagca ggaaaccctg
      121 cgcgaagtcg acgtccagcg cgcgcagccg ctccagcacc gacggcttgt cgacgaactc
      181 ggccaccgtg cgcaggccca tgaccttggc gacatcggcg aagcagcgca ccgcggcctc
      241 gtcgagcgcg tcgtcgacga ggtcgcgcac gaactggccg tcgatcttga gcgtgtctac
      301 cctcagggtc ttgaggtagc cgaaggatgc ggcgccggcg ccgaagtcgt ccagcgccac
      361 ctgcacgccg atcgcgcgca cctcgtcgac gaaggccgcg gcgtcggcca tgcgcgtgac
      421 cgcggcggtc tcggtgatct ccaggcacag gcgcccgcac acgcgcgggc cggcgcggcc
      481 gaggagctcc agcgcccagc gatggaacgt gcggtcgccc accgactgtc ccgagaggtt
      541 gacgctcagc agctcgatga ggcccggctc gcgcagcgcg atcatccagt cgaccacgcg
      601 ccgcagcacc cagcggtcga tgcgcgaggc gaggtggaag cgctcggccg ccggcaggaa
      661 cgcccccggc gcgacgagcg agccgtccgc ctgcttcatg cgcagcagca cctcggcgtg
      721 cacgcccgcg gtcgcgtgct tgagcggttc gatgcgctgc gcgaacaggg cgaagccgtc
      781 ctcgtcgagc gcctgctcga tgcgcgccgt ccactgcatc tcgaactggc gctcgcgcag
      841 ggcggcgtcg ctgtccatcc agacgtgcac ccggttgcgg ccggcctcct tggcggcgta
      901 gcacgagctg tcggccgcct gctggatggc cgcgacgctc gtccagcggc gatcgaccgg
      961 caccagcccg atgctcgtgc cgatgcgaaa gcgccgctcc tcgtggatga agcggaagtc
     1021 ctccatgcgc tcgcacagcc tctgcgcggt gtgcagcgcg cgctcggcgc cgcagtgctc
     1081 catgatgatc gcgaactcgt cgccgcccag tcgcgcgacg gtgtcgcggg cgcgcacggc
     1141 atcggcgagc atgcgcgcca tctgctgcag cagcacgtcg cccaccgagt ggccgcaggc
     1201 atcgttcacg agcttgaact ggtcgaggtc gatgtacagc agcgcgtgct cgctcgcgtc
     1261 ctcgtgggac ttgtccagca cgcgcttcag gcgcgcctcg aattccgcgc ggttgacgag
     1321 gcccgtcagc gcgtcgtggg tcgcgcgcca gttcatctcg cccgagagcc ggcgctgctc
     1381 gctgacgtcg tggaagacga tgacgacgcc cagcacctcg cccgcctcgt cgcggatcgg
     1441 cgccgccgag tcctcgatgc cgtactcccg cccggcgcgc gagaccagca gcgacggcgc
     1501 cacgttgcgc tccgtgcgtc cgggcgccag gcagcccgcc accgggtcgg gcgcgggctc
     1561 gcgcgtgcgc tcgtcgacga cgcggaagac ctggcccagt ggacggtcgc gcgcctccgc
     1621 cgagtcccag ccggtgaggc gctcggccac cgggttgagc cacgtgatca tcccgtgggc
     1681 gtcggtggtg atgacggcgt cgccgatcga ctccagcgtg acgcgcagca gctggcgatc
     1741 ctcggcgagg tcggccgcga gctggcgcag ctccgtgatg tcccaggtcg cgccgatcag
     1801 gcggcgcggg cggccgctcg cgtcgtgcgt cacgcgcgcg gtgccgcgca ggtggcgcgt
     1861 cgagccgtcg gccatcacga gccggaactc gcgatccagc ggtgcgccgc cggccgcggc
     1921 ctcgcggatg gcgaggtcga tgccggcgat gtcgtcggga tgcatccgct ggtcccacag
     1981 cgtcaccggc gaggcgcggc cgtcggcggc gtcgccgtac aacgcgtgca tccgcttgtc
     2041 ccacagcagc tcgccggtgt cgaggtcgag ctcccagatg ccgatgccgc cgctctcggt
     2101 ggccagcgcg atgcgctcgt tggcctccgt gagcgcctcg cgctgggcga cgagctcggt
     2161 gacgtcctgg atcgcgccga ccagccgcgc cggctcgccc tgcacgcgct cgaccgccgc
     2221 gacgacatgc accgtgatcc gccggccgtc ggcgcgcacg aggggcatct cgacgtcgta
     2281 gggcgtgccg tcggtgatcg cctgctgcac gaggccgatc acgcgcgcct ggtacggcgc
     2341 cgggtagaag tccagcgccg ccgcgaagct gggcgcgtag ccgggcggca cgccgtggat
     2401 ccgcttggtc tcgtccgacc atacgagcgc catcgactcg acatcgagct cccagccgcc
     2461 gatgccgccg agctggccgc tgcggtcgag gaacagctcg ctcttgcgca gcgcctcttc
     2521 ctggcgcttg cgctcggtga tgtcgaacgc gacggacagg tagcccgtca ccccgccggc
     2581 ggcgtcgtgc tcggggatca ggctctgctg catccagtgc ggctcgccgc gcatgacggc
     2641 gacggcctcg aagtcgcgcc gctcgccgcg cagcacggcc tcgacatgcg gcgcgagcac
     2701 ctcccactcg gcctcgcccc ggaacgcgcg ctgcgagctg cccacgagcg aggcgggatc
     2761 gacgccgtag aactcgcgga agcgctcgtt ggcgtaggtg caggtgaggc tgcggtcgaa
     2821 gcgcgtgacg gtcgcgggca ggttgtcggt gatcgtgcgc aggcggcgtt cgccggtcgc
     2881 cagcgccgtc tcggcgcgct tgcgttccgt gatgtccatc gtcatcacgt agaagccgtc
     2941 gacgcagccc gcggggttga agtccggcag caggttgacg tcgaaggtgg cgtcgggacg
     3001 gcggccgatc cccacgagct cgaagcgctg cggctcgccg cgcagcgcgg cggccaccgg
     3061 cgccgcgacc ttcgcgtacg cggccacgcc cagcatctgc tccagcgtgc ggccgatgaa
     3121 ctccgactgg gggatgccga acacggcctc gtaggtggcg ttgacgaagc gatagacctg
     3181 gttcgcgtcg acatggccga tcagcgcggg gacgctgtcg gtgaccatgc gcagccggcg
     3241 ctcgccgcgc gcgagctcga gctccgcggc cttgcgcgcg gtgacgtcca cggccatcac
     3301 gtagaagccc tcgacgccgc cggtcgggtt gatgtcgggc aggtagctcg tctcgagcac
     3361 gatgtccgcc gcgcgcccga tgccgtggtg ctcgaaggtg cgggtctggc cggccagcgc
     3421 cgcgcggacg tgttcctcga cccgcgcgta cgtggccggg cccagcacct cggccatcgt
     3481 gcggccgacg gtggcgtcgg ggtcgacgcc gaagatcggc cggtaggccg cgttgaggaa
     3541 gcggtaggcc tcgtggcggt cgacgtagcc gatcagcgcg ggcaggttgt ccgtgatggc
     3601 cttcagccgg cgctcgctca gctccagccg gttgcggccc tcgcgcaggt cgtcggcgag
     3661 cgcggcgcgc aggcgcagct cgcgccgcgt gagcacggcg gc
//
LOCUS       DASVLN010000563         3112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_515333, whole genome shotgun sequence.
ACCESSION   DASVLN010000563 DASVLN010000000
VERSION     DASVLN010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515333"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF453_13540"
     CDS             complement(<1..372)
                     /locus_tag="VF453_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7688729.1"
                     /translation="MPALFSLDRSSPVALVDQIEAHLRELIERGQLPAGARLTSIRQL
                     AGELAVSPNTVIAAYDRLVAAGAIDARGTAGYFVNPARGGASAQDAALEAGEEQEPVW
                     VAQQANDQRAGVLLASSGALPP"
     gene            complement(382..984)
                     /locus_tag="VF453_13545"
     CDS             complement(382..984)
                     /locus_tag="VF453_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7688730.1"
                     /translation="MTATPVPPQLLPMMLYCFAMSITPGPNNMLLTASGANFGYRLTL
                     PQILGINAGGFVLATASCVGLGQLFVAWPALQAVLRIGGALYLLYLAAKLARARIGDA
                     TMLRPQSFVEGALFQVINPKSWLKAITLGSVFMPAGASPLPAALAIATVSALIGLPCV
                     SLWALFGVAIRRFLQDARNQRLFNLAMAATLAVLALDFLR"
     gene            complement(1192..2751)
                     /locus_tag="VF453_13550"
     CDS             complement(1192..2751)
                     /locus_tag="VF453_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7688731.1"
                     /translation="MFQNLGFKHKIGALVGVSVAGLCVFAAVSLVELRDGIVDGRKRQ
                     LVAAVDSARSIAASYQALAASGSMSEADAQKAAKDALRGQRYGKALADYVYVFSRQGD
                     GVMHPFKRDWDGQHMLGKVKDGDGMDIVQGVVDASSGPTGAGFFMTKFPRPGQTTPVP
                     KLQYVEALKGWDWVIGSGVYTDDVDAEVRSALLEGLGIAAALLLAIGAIGFLVARSVL
                     RQIGGDPSDALRAMDEVARGDLSTDVRTDVPGSLLAGLSQMIVSLRTTVSQVRHATDS
                     IATASGQIAAGNHDLSARTEQTASNLQQTAASMEELTGTVNQTSDSARKANELAGSAT
                     AAANKGGEVVSQVVATMDDINASSKRIGDIIGVIDGIAFQTNILALNAAVEAARAGEH
                     GRGFAVVASEVRSLAQRSAGAAKEIKTLIAASVERVDSGSRLVAAAGSSMTDIVSSVG
                     RVSAIIGEITNAAGEQSSGIGQVNGAVSQLDQMTQQNAALVEESAAAAESLKEQARRL
                     AEVVEVFRLER"
     gene            complement(2880..>3112)
                     /locus_tag="VF453_13555"
     CDS             complement(2880..>3112)
                     /locus_tag="VF453_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX7688732.1"
                     /translation="PPAPLPAAAAPASAPADPLAEPVAIAVGVLMHRYSLSRLEAYER
                     LQRMAATDGLQVKVQAERLLDAVELLARPASA"
BASE COUNT          428 a         1111 c         1152 g          421 t
ORIGIN      
        1 cggcggcagc gcgccgctgc tggcgagcag cacgcccgcg cgctggtcgt tggcctgctg
       61 ggcgacccag acgggctcct gctcctcgcc ggcctcgagc gcggcgtcct gcgccgaggc
      121 gccgccgcgt gccgggttga cgaagtagcc ggccgtgccg cgcgcgtcga tcgcgcccgc
      181 ggccaccaga cggtcgtagg ccgcgatgac ggtgttggga ctgacggcca gctcgccggc
      241 gagctggcgg atcgaggtca ggcgcgcccc ggcgggcagc tggccgcgct cgatcagctc
      301 gcgcaggtgc gcctcgatct ggtcgacgag cgcgaccggc gagctgcggt cgaggctgaa
      361 cagcgcgggc atgcggcggc ctcagcgcag gaagtcgagg gcgaggacgg ccagcgtcgc
      421 ggccatcgcg aggttgaaca ggcgctggtt gcgcgcgtcc tgcaggaagc gccggatcgc
      481 cacgccgaag agcgcccaca gcgacacgca gggcaggccg atcagcgcgc tcacggtggc
      541 gatggccagc gcggcgggca gcggcgacgc gccggcgggc atgaagaccg agcccagcgt
      601 gatcgccttg agccagctct tggggttgat cacctggaac agcgcgcctt cgacgaagga
      661 ctgcgggcgc agcatcgtcg cgtccccgat gcgcgcccgc gccagcttgg ccgccaggta
      721 cagcaggtac agcgcgccgc cgatgcgcag cacggcctgc agcgcgggcc aggcgacgaa
      781 cagctggccc aggccgacgc acgatgccgt cgcgagcacg aagccgccgg cgttgatgcc
      841 caggatctgc ggcagcgtga gccggtagcc gaagttcgcg cccgaggcgg tcagcagcat
      901 gttgttgggc ccgggcgtga tcgacatcgc gaaacaatac agcatcatcg gcaacagctg
      961 gggcggcacg ggggtggcgg tcacgtcagg atctccttgg ccgccagcgt aggttcccgt
     1021 ccccggccgg ccaaggcaca agccggcgat cgcgcgacgc gctgtatcaa tccgccgacc
     1081 gcaacagccg gccccgctcc ggcaagcgga cgcgccgacg cagggccgcg gggccctcgt
     1141 cggcgtcgcg ccggatcgcg cgagcggcga accctcgccg tcccgcgggc gtcagcgctc
     1201 cagccggaac acctcgacca cctcggccag ccggcgcgcc tgttccttca ggctctccgc
     1261 cgccgcagcc gattcctcga cgagcgccgc gttctgctgc gtcatctggt ccagctgcga
     1321 gaccgcgccg ttgacctggc cgatgccgga ggactgctcg cccgcggcgt tcgtgatctc
     1381 gccgatgatg gcgctcacgc ggccgacgct ggagacgatg tccgtcatcg acgagcctgc
     1441 cgcggcgacc agccgcgagc ccgagtcgac gcgctcgacc gacgccgcga tcagcgtctt
     1501 gatctccttg gccgcgcccg ccgagcgctg ggcgaggctg cgcacctcgg acgccaccac
     1561 cgcgaagccg cggccgtgct cgcccgcgcg cgctgcctcg acggccgcgt tcagcgcgag
     1621 gatgttggtc tggaacgcga tgccgtcgat gacgccgatg atgtcgccga tgcgcttgga
     1681 gctcgcgttg atgtcgtcca tcgtggcgac gacctgcgag accacctcgc cgcccttgtt
     1741 cgcggcggcg gtcgccgagc cggcgagctc gttggccttg cgcgccgagt cggacgtctg
     1801 gttgacggta ccggtcagct cctccatcga agcggcggtc tgctgcaggt tggacgcggt
     1861 ctgctcggtg cgcgccgaga ggtcgtggtt gccggccgcg atctggcccg acgcggtcgc
     1921 gatcgagtcg gtcgcatggc gcacctgcga gacggtcgtg cgcagcgaga cgatcatctg
     1981 cgagaggccg gccagcaggc tgcccgggac gtccgtgcgg acgtcggtgg agaggtcgcc
     2041 gcgggcgact tcgtccatcg cgcgcagggc gtccgacggg tcgccgccga tctggcgcag
     2101 cacgctgcgc gcgaccagga agccgatggc gccgatggcc agcaggagcg ccgcggcgat
     2161 gcccaggccc tccaggagcg cggagcgcac ctcggcatcg acgtcgtcgg tgtagacgcc
     2221 cgagccgatc acccagtccc agcccttcag ggcctcgacg tactgcagct tgggcaccgg
     2281 cgtggtctgg ccggggcgcg ggaacttcgt catgaagaag ccggcgcccg tcgggccgct
     2341 ggaggcgtcg acgacgccct ggacgatgtc catgccgtcg ccgtccttga ccttgcccag
     2401 catgtgctgg ccgtcccagt cccgcttgaa gggatgcatg acgccgtcgc cctgcctcga
     2461 gaagacatag acgtagtcgg cgagcgcctt gccgtagcgc tgcccgcgca gtgcgtcctt
     2521 ggccgccttc tgcgcgtcgg cctcggacat cgagccggac gccgcgagcg cctggtagga
     2581 cgcggcgatg ctgcgcgcgg agtccaccgc cgcgacgagc tggcgcttgc ggccgtcgac
     2641 gatgccgtcg cgcagttcta cgagcgagac ggccgcgaac acgcacaggc ccgcgacgga
     2701 gacgcccacg agggcgccga tcttgtgttt gaagccgagg ttctggaaca tggcgatcga
     2761 tgacggggtg ggccgctgac gcggttcgaa cccgcttcat cggccaccca cgctcggcgc
     2821 ctcaatcgag attgactatc gcccggccgg ggctgcccgg cctcgcgcct tgtcgtcgct
     2881 caggcgctgg cgggccgcgc caggagttcg accgcgtcca gcagtcgctc cgcctgcacc
     2941 ttcacctgca ggccgtcggt cgcggccatg cgctgcaggc gctcgtaggc ctcgaggcgc
     3001 gagagcgagt agcggtgcat caggacgccc accgcgatgg cgacgggctc ggcgagcggg
     3061 tcggccggcg cggacgccgg ggccgcggcg gcgggcaacg gcgcgggcgg cg
//
LOCUS       DASVLN010000564         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_299848, whole genome shotgun sequence.
ACCESSION   DASVLN010000564 DASVLN010000000
VERSION     DASVLN010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299848"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VF453_13560"
     CDS             complement(<1..546)
                     /locus_tag="VF453_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEX7688733.1"
                     /translation="MTLKRRDVLLRGGSLALALSASDLAFGATILAVRVWPAADYTRV
                     TIESDQPLIAHNFMAEAPARLVVDIDNLELSPELRELVGKVRPDDPYIAGVRIGQNTP
                     KVVRLVLDLKQPVNPQQFALAPVAAYQNRLVFDLYPSQAPDPLLGLVRGKEHAEQAAA
                     QSVQDALGEFIGRIPGGASGPA"
     gene            complement(543..1037)
                     /gene="tsaE"
                     /locus_tag="VF453_13565"
     CDS             complement(543..1037)
                     /gene="tsaE"
                     /locus_tag="VF453_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7688734.1"
                     /translation="MTQSHPPILESLTLAWPDEAACADTARRLARHLALRDAFVELDG
                     PLGAGKTTFARHLLHALGVEGRVKSPTYAVVETYDLPASPPVAHFDFYRFDDPQEWED
                     AGLRELFAAPGLKLVEWAEKAEPLLPAPDLRVTITPHDSGDRGVRIDAMTPKGLQLLD
                     NRAP"
     gene            1158..>2110
                     /gene="queG"
                     /locus_tag="VF453_13570"
     CDS             1158..>2110
                     /gene="queG"
                     /locus_tag="VF453_13570"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX7688735.1"
                     /translation="MAAVHLGDEAEAGLRAWLDAGFHGSMGYMAAHGMKRARPAELHP
                     GTVRVITARMDYLPATRGEDWRGEEQARLARPAEAVVSVYARGRDYHKVLRHRLQQLA
                     DRLADAIGPLGHRCFTDSAPVLEVELAARSGLGWRGKHTLALARDAGSMFFLGEIFVD
                     LALPLTAAAQPHCGSCTACIDVCPTQAIVGPQRLDARRCISYLTIEHDGPIPHEFRRA
                     LGNRIYGCDDCQVACPWNKYARRSPLPDFAEREHLRQPTLLELFGWSEDEFLRRTEGS
                     PIRRIGHERWRRNLAVALGNALHAGEPRAPIEAALRAALPGA"
BASE COUNT          272 a          765 c          785 g          288 t
ORIGIN      
        1 tgccgggccg ctcgcgccgc cggggatgcg gccgatgaac tcgcccagcg cgtcctgcac
       61 cgactgcgcg gcggcctgct cggcgtgctc cttcccacgc acgaggccca gcagcgggtc
      121 gggtgcctgc gacgggtaga ggtcgaacac gagccggttc tggtacgccg cgaccggcgc
      181 gagggcgaac tgctggggat tgaccggctg cttcaggtcg agcacgagcc gcacgacctt
      241 gggcgtgttc tggccgatgc gcacgccggc gatgtacggg tcgtcgggcc gcaccttgcc
      301 gacgagctcg cgcagctccg ggctgagctc gaggttgtcg atgtcgacga cgaggcgggc
      361 gggcgcctcg gccatgaagt tgtgcgcgat gagcggctgg tcggactcga tcgtgacgcg
      421 cgtgtaatcg gcggccggcc agacgcgcac cgccaggatc gtcgcgccga acgcgaggtc
      481 gctcgcgctc agggccagcg cgaggctgcc gccgcgcagg aggacgtctc ttcgcttcaa
      541 cgtcatggcg cccgattgtc gagcaattgc aggcctttcg gggtcatcgc gtcgatccgc
      601 acgccccgat cgccgctgtc gtgtggcgtg atcgtcacgc gcaggtccgg ggcgggcagc
      661 agcggctcgg ccttctcggc ccattcgacc agcttcagcc cgggcgccgc gaacagctcg
      721 cgcaggccgg cgtcctccca ctcctgcggg tcgtcgaagc ggtagaagtc gaagtgcgcg
      781 accggcggcg aggcgggcag gtcgtaggtc tcgacgaccg cgtaggtcgg gctcttcacg
      841 cgcccctcga cgcccagcgc gtgcaggagg tggcgcgcga aggtggtctt gccggcgccg
      901 agcgggccgt cgagctcgac gaacgcgtcc cgcagcgcga gatgccgcgc gagccggcgc
      961 gcggtgtcgg cgcacgccgc ctcgtcgggc caggcgagcg tcaggctttc tagaatcggg
     1021 ggatgcgact gcgtcatgag ggcggggaga tcgatccccg gcaagccgag gaagttccgg
     1081 gcgaggatcc gcggggggcg gacctcctcc gccgcctgca gggctgggcc cgcgagctcg
     1141 gattctcaca gatcggcgtg gccgcggtcc acctcggcga cgaggccgag gccggcctgc
     1201 gcgcgtggct ggacgcgggc ttccacggca gcatgggcta catggcggcc cacggcatga
     1261 agcgcgcacg gcccgcggag ctgcaccccg gcaccgtgcg cgtcatcacg gcgcgcatgg
     1321 actacctgcc cgcgacccgc ggcgaggact ggcgcggcga ggagcaggcc cgcctcgcgc
     1381 gcccggccga ggccgtcgtg tccgtctacg cccgcggtcg cgactatcac aaggtgctgc
     1441 gccaccgcct gcagcagctc gccgaccgcc tggccgacgc gatcggcccg ctcgggcatc
     1501 gctgcttcac cgattcggcg ccggtgctgg aggtcgagct cgcggcccgc tcgggcctgg
     1561 gctggcgcgg caagcacacg ctcgcgctcg cgcgcgacgc gggctcgatg ttcttcctcg
     1621 gcgagatctt cgtcgacctc gcgctgcccc tgaccgccgc cgcgcagccg cactgcggca
     1681 gctgcaccgc ctgcatcgac gtctgcccga cgcaggccat cgtcgggccg cagcggctcg
     1741 acgcgcgacg ctgcatctcg tacctgacga tcgagcacga cgggccgatc ccgcacgagt
     1801 tccggcgcgc gctcggcaac cggatctacg gctgcgacga ctgccaggtc gcctgtccgt
     1861 ggaacaagta cgcgcgtcgc agcccgctgc ccgacttcgc cgagcgcgag cacttgcggc
     1921 agccgacgct gctcgagctg ttcgggtgga gcgaggacga gttcctgcgc cgcacggaag
     1981 gctcgccgat ccgccgcatc ggccacgagc gctggcgccg caacctggcc gtggcgctgg
     2041 gcaacgcgct gcacgccggc gagccgcgcg cgcccatcga ggccgcgttg cgcgcggcgc
     2101 tgcccggggc
//
LOCUS       DASVLN010000565         5151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_244100, whole genome shotgun sequence.
ACCESSION   DASVLN010000565 DASVLN010000000
VERSION     DASVLN010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244100"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VF453_13575"
     CDS             complement(<1..193)
                     /locus_tag="VF453_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7688736.1"
                     /translation="MPRTRAASPTADTRADAWLALDKQLCFALYSASLAMTKMYKPLL
                     APLGLTYPQYLVMLALWEED"
     gene            330..716
                     /locus_tag="VF453_13580"
     CDS             330..716
                     /locus_tag="VF453_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7688737.1"
                     /translation="MPEPAVLDRVFHALSDPSRRAIVERLAGGDASVSELARPLPMSL
                     AAVVQHVQALEACGLVRSTKTGRVRRCSLDRGVLALAEGWLTQRRAFWNAQLDRLGEL
                     LVEDAAGAPAAPAPATRSTRKKGTPR"
     gene            713..1192
                     /locus_tag="VF453_13585"
     CDS             713..1192
                     /locus_tag="VF453_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010096195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7688738.1"
                     /translation="MTHPTPARHPSAHGQFTLLRAYAAAPARVYAAWADPAIKARWFI
                     GPNQWQEIERTLDVRVGGVETLRGRFPDGLEPLFTARYHLVEPDTRLVYVYDMHMSGR
                     HHSVSLATVEFTPAGSGCELRFTEQVVFVDGTDGREGTLSRERGTAAHLDRIAPVIS"
     gene            complement(1465..2088)
                     /gene="pssA"
                     /locus_tag="VF453_13590"
     CDS             complement(1465..2088)
                     /gene="pssA"
                     /locus_tag="VF453_13590"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX7688739.1"
                     /translation="MEQQKPKPFSMIREFHLADWFTLANAFCGVGALFSVMSFLQSRD
                     IRHIYFACALIPAALVFDVLDGRIARWRQQSSEMGRELDSLADVISFGVAPAAIAYGC
                     GMQGLYDRIVLVFFVVCGVSRLARYNITAEKLAGATGKVKYFEGTPIPTSLLLVVVLA
                     VAANVGAVAERLWFGAVDLGGFHLHPLVLMFAISGSLMISRIRIRKL"
     gene            complement(2152..3654)
                     /gene="glpK"
                     /locus_tag="VF453_13595"
     CDS             complement(2152..3654)
                     /gene="glpK"
                     /locus_tag="VF453_13595"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX7688740.1"
                     /translation="MTSRRFLLALDQGTSSSRSIVFDDQGRAVASAQREFRQIFPRPG
                     WVEHDPKEILESQLATAREAIAKAGLQARDIASIGITNQRETTLVWNRATGEPIHNAI
                     VWQDRRSEPACAALREAGHEAAIRAKTGLVVDAYFSGTKLKWILDNVDDARAAARRGE
                     LAFGTVDTWLIWHLTGGRVHATDVTNASRTMMLDVHRNAWDDELLALLDVPRSLLPEV
                     KPSSHVFGHTRQDLLGASLPIGGVAGDQQSALFGQACFRPGLAKNTYGTGCFMLMHTG
                     TDFRVSANGLVTTSAAQVRATPEFAFEGSVFIGGAVVQWLRDGLRAIQGSGEVQRLAE
                     SVPNSGGVMFVPAFTGLGAPYWDADARGAIVGLTRGSTVGHIARAALESIAFQSAALL
                     AAMSRDAVAAGGAPVAELRVDGGACVNDLLMQFQADLLGIPVVRPQVIETTALGAAYL
                     AGLSSGVFAGLDELAAQWRAERTFLPTMSRDEAGALMQAWEHAVRQATTR"
     gene            complement(3651..4430)
                     /locus_tag="VF453_13600"
     CDS             complement(3651..4430)
                     /locus_tag="VF453_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7688741.1"
                     /translation="MTPTPRQLELIDEVRRRQSVTVEALAERFGVTLQTVRRDVRQLT
                     DAGLLARFHGGVRLPGSTTENIEYRKRQQLNAASKQRIARSVAQRVPHGSSLLINIGT
                     TTEAIARELLQHKGLRVITNNLNVAAILSDNADCEVIVAGGVVRARDRGIVGEVTVDF
                     IAQFKVDIGLIGISGIEDDGTLRDFDYREVKVARAILAHSREVWVAADHSKFNRPAMV
                     ELARFDQVDMLFTDAPPPSPFPTLLAQAGVTCVDCHEDAAP"
     gene            4479..>5151
                     /locus_tag="VF453_13605"
     CDS             4479..>5151
                     /locus_tag="VF453_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7688742.1"
                     /translation="MPKSENETNTIAANRKNASMSSNSTQHLDSNVQDGSPAAAPTPG
                     GAAVPASCDVLVVGGGINGAGIARDLAGRGLRVLLCEQGDLAQATSSASTKLIHGGLR
                     YLEYREFSLVRKALAEREVLLRAAPHIMWPLRFVMPHDPGMRPVWMIRAGLFLYDHLA
                     RREVLPASRTVDLRRHAAGVPLQRRFTKGFVYTDGWVDDARLVVLNALDAAERGATVL
                     TRRRCE"
BASE COUNT          764 a         1843 c         1838 g          706 t
ORIGIN      
        1 cgtcctcctc ccacagcgcg agcatgacca ggtactgcgg ataggtcagg cccagcggcg
       61 cgagcagcgg cttgtacatc ttcgtcatcg cgagcgaagc cgagtacagc gcgaagcaca
      121 gttgcttgtc gagcgccagc cacgcgtcgg cgcgggtatc ggcggtcggg gaagcggcgc
      181 gggtgcgagg catggggtga aatgtagcgg gcatttaaat tgtgcacaat cgaaatgggt
      241 attggacgca gcggcccgct ggctcgggat cgtgcgcttc actaaagcaa atgcttgact
      301 gtcagcggtg gcctgcgcat actgcgacca tgcccgagcc cgccgtcctc gaccgcgtct
      361 tccacgccct gtccgatccg tcgcggcgcg ccatcgtcga gcgcctggcc ggcggcgacg
      421 ccagcgtgag cgagctcgca cggccgctgc ccatgtcgct cgcggccgtc gtgcagcacg
      481 tgcaggcgct cgaggcgtgc gggctcgtgc gcagcaccaa gaccggccgc gtgcgccgct
      541 gcagcctcga ccgcggcgtg ctcgccctgg ccgagggctg gctcacgcaa cggcgcgcct
      601 tctggaacgc gcagctcgac cgcctcggcg aactgctggt cgaggatgcg gccggggcgc
      661 ccgccgcccc ggcgccggcg actcgatcca cccgcaagaa aggaaccccg cgatgaccca
      721 tccgaccccc gcccgccatc cgtccgcgca cggccagttc acgctgctgc gcgcctacgc
      781 cgccgcgccg gcccgcgtct acgcggcctg ggcggatccc gcgatcaagg cgcgctggtt
      841 catcggcccg aaccagtggc aggagatcga gcgcacgctc gacgtgcgcg tgggcggggt
      901 cgagacgctg cgcggccgct tccccgacgg cctcgagccg ctgttcacgg cgcgctacca
      961 cctcgtcgag ccggacaccc gcctggtcta cgtctacgac atgcacatgt cgggcaggca
     1021 ccactcggtc tcgctggcga cggtggagtt cacgcccgcc ggctcgggct gcgagctgcg
     1081 cttcacggag caggtcgtct tcgtcgacgg caccgacggc cgcgagggca cgctctcacg
     1141 cgaacgcggc accgcggcac acctcgaccg catcgcgcca gtcatctcgt gacgagcggc
     1201 gcccgccagc gacgcgcccg gaaaatgcgc gagcgcagcg acgcgccccc cgcgcccccc
     1261 gcgccccggg cggcaattgc gcgagcgcgg cgacgcgccc cccgtgcccc gccgaaaatg
     1321 cgcgagcgca gcgacgcgcc ccccgtcgcc gacgctgtcg ctcgtctctc ccgaacgaga
     1381 cacggggaag cggcagatcc ggctccgccg gtctgctcgc gcacgccccc tcggggggca
     1441 gggagcgcaa gcgaccgtgg gggctcacaa cttccggatg cggattcggc tgatcatcag
     1501 cgacccggag atcgcgaaca tcagcaccag cggatgcagg tggaagccgc cgaggtcgac
     1561 ggcgccgaac cagagccgct cggccaccgc gccgacgttg gccgcgaccg cgagcaccac
     1621 gaccagcagc agcgaggtcg ggatcggcgt gccctcgaag tacttcacct tgccggtggc
     1681 gccggccagc ttctcggccg tgatgttgta gcgcgcgagg cgcgagacgc cgcacacgac
     1741 gaagaacacg agcacgatgc ggtcgtagag cccctgcatg ccgcagccgt aggcgatggc
     1801 cgctggggcg acgccgaagg agatgacgtc ggcgagcgag tcgagctcgc ggcccatctc
     1861 cgagctctgc tggcgccagc gcgcgatgcg cccgtcgagc acgtcgaaca cgagcgcggc
     1921 cgggatcagc gcgcacgcga agtagatgtg gcggatgtcg cgcgactgca ggaagctcat
     1981 gaccgagaac agcgcgccga cgccgcagaa ggcgttcgcg agcgtgaacc agtccgcgag
     2041 gtggaactcg cggatcatcg agaagggctt gggcttttgt tgttccatcc ggcaagcata
     2101 gcgaggacgc ggcggtccgt cgtgcaacgg acgtgcccgc gcggcagccg ctcagcgcgt
     2161 cgtcgcctgc cgcaccgcgt gctcccacgc ctgcatcagg gcgccggcct cgtcgcgtga
     2221 catcgtcggc aggaaggtgc gctcggcgcg ccactgggcg gcgagctcgt cgagtcccgc
     2281 gaacacgccg ctcgacaagc ccgccaggta ggcggcgccg agcgcggtcg tctcgatcac
     2341 ctgcggacgc acgacgggaa tgcccagcag gtcggcctgg aactgcatca gcaggtcgtt
     2401 gacgcacgcg cccccgtcga cccgcagctc ggccaccggc gcgccgccgg cggcgaccgc
     2461 gtcgcggctc atcgcggcga gcagcgcggc gctctggaac gcgatcgact ccagcgccgc
     2521 gcgcgcgatg tggccgaccg tgctgccgcg cgtcagcccg acgatggcgc cgcgcgcatc
     2581 ggcgtcccag tagggcgcgc ccaggcccgt gaacgcgggc acgaacatca cgccgccgct
     2641 gttgggaacg ctctcggcca ggcgctgcac ctccccgctg ccctggatcg cgcgcaggcc
     2701 gtcgcgcagc cactgcacca ccgcgccgcc gatgaagacg ctgccctcga acgcgaactc
     2761 cggcgtcgcg cggacctgcg cggcgctggt ggtgacgagg ccgttggccg agacgcggaa
     2821 gtccgtgccc gtgtgcatca gcatgaagca gccggtgccg taggtgttct tcgcgaggcc
     2881 cggccggaag caggcctggc cgaagagcgc gctctgctgg tcgccggcga cgccgccgat
     2941 cggcaggctc gcgcccagca ggtcctgccg cgtgtggccg aacacgtggc tcgacggctt
     3001 cacctcgggc aacaggctgc gcggcacgtc cagcagcgcg agcagctcgt cgtcccacgc
     3061 gttgcgatgc acgtcgagca tcatcgtgcg cgacgcattg gtgacgtcgg tcgcatgcac
     3121 gcggccgccg gtcaggtgcc agatcagcca ggtgtcgacg gtgccgaaag cgagctcgcc
     3181 gcgccgcgcg gcggcgcgcg cgtcgtcgac gttgtccagg atccacttga gcttggtgcc
     3241 cgagaagtag gcgtcgacga cgaggcccgt cttcgcgcgg atcgccgcct cgtgcccggc
     3301 ctcgcgcagt gcggcgcagg cgggctcgga gcggcggtcc tgccacacga tcgcgttgtg
     3361 gatcggctcg cccgtcgcgc ggttccacac cagcgtcgtc tcgcgctggt tggtgatgcc
     3421 gatcgaggcg atgtcgcgcg cctgcaggcc ggccttcgcg atcgcctcgc gtgcggtggc
     3481 cagctgcgac tcgaggatct ccttcgggtc gtgctcgacc cacccggggc gcgggaagat
     3541 ctggcggaac tcgcgctgcg ccgacgcgac ggcgcggccc tggtcgtcga agacgatgct
     3601 gcgcgagctg gaggtgccct ggtccagggc gagcaggaag cggcgggagg tcatggggcg
     3661 gcgtcctcgt ggcagtcgac gcaggtcacg ccggcctgcg cgagcagggt ggggaagggc
     3721 gacggcgggg gcgcatcggt gaagagcatg tcgacctggt cgaagcgggc gagctccacc
     3781 atcgccggcc ggttgaactt gctgtggtcg gccgcgaccc agacctcgcg cgagtgggcg
     3841 aggatggcgc gcgcgacctt gacctcgcgg tagtcgaagt cgcgcagcgt gccgtcgtcc
     3901 tcgatcccgg agatgccgat caggccgatg tcgaccttga actgcgcgat gaagtcgacc
     3961 gtcacctcgc cgacgatgcc gcggtcgcgc gcgcgcacga cgccgccggc cacgatcacc
     4021 tcgcaatcgg cgttgtccga caggatcgcg gccacgttca ggttgttcgt gatcacgcgc
     4081 aggcccttgt gctgcagcag ctcgcgcgcg atcgcctcgg tcgtcgtgcc gatgttgatc
     4141 agcagcgatg agccgtgcgg cacccgctgc gcgaccgatc gcgcgatgcg ctgcttggat
     4201 gccgcgttga gctgctggcg cttgcggtac tcgatgttct cggtggtcga gcccggaagc
     4261 cgcacgccgc catggaagcg cgcgagcagc ccggcgtcgg tgagttggcg gacgtcgcgc
     4321 cgcacggtct gcagcgtgac cccgaagcgc tcggccagcg cctccacggt cacgctctgg
     4381 cggcgtcgga cctcgtcgat cagctcgagc tggcgggggg tgggagtcat cgcgggcgaa
     4441 tctaaacgat caaaaccgaa tcgactcgag ggttttccat gcccaaaagc gaaaatgaaa
     4501 cgaacacaat cgccgcgaac cgaaaaaacg caagcatgag ttcaaactcg actcagcacc
     4561 tggacagcaa cgtgcaagac ggatcccccg ccgccgcgcc gacgcccggc ggcgcggcgg
     4621 tgcccgcgtc gtgcgacgtg ctcgtcgtcg gcggcggcat caacggggcc ggcatcgccc
     4681 gcgatctcgc cggccggggc ctgcgcgtgc tgctgtgcga gcagggcgac ctcgcgcagg
     4741 cgacgtcgtc ggcgtcgacc aagctcatcc acggcggcct gcgctacctc gagtaccgcg
     4801 agttctccct cgtgcgcaag gcgctggccg agcgcgaggt gctgctgcgc gcggcgccgc
     4861 acatcatgtg gccgctgcgc ttcgtgatgc cgcacgaccc gggcatgcgt ccggtctgga
     4921 tgatccgcgc cgggctgttc ctctacgacc acctcgcgcg gcgcgaggtg ctgccggcct
     4981 cgcgcacggt cgacctgcgc cgccacgccg cgggcgtgcc gctgcagcgg cgcttcacca
     5041 agggattcgt ctacaccgac ggctgggtcg acgacgcgcg gctggtcgtc ctcaacgcgc
     5101 tggatgccgc cgagcgcggc gcgaccgtgc tgacgcgccg gcgctgcgag g
//
LOCUS       DASVLN010000566         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_244166, whole genome shotgun sequence.
ACCESSION   DASVLN010000566 DASVLN010000000
VERSION     DASVLN010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244166"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VF453_13610"
     CDS             complement(<1..701)
                     /locus_tag="VF453_13610"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7688743.1"
                     /translation="MQAANYMGPPDGADDGPDARPDEAEPDHDAAEVRIASIGLERHG
                     ERLDKVLTTLAPEFSRAHLQGLIADGRVALDGRPATTASTRVKAGQQVRVELVRPAHE
                     LAFRAEPIPLAIVHEDEHVLVLDKPAGLVVHPAAGNWSGTLLNGLLAHDPASAALPRA
                     GIVHRLDKDTSGLMVVGRSLEAVTGLVRDIAARAVSRRYLALAHGRLAQDEDTIEAPI
                     GRDPRVRVRMAVVAGG"
     gene            750..1571
                     /locus_tag="VF453_13615"
     CDS             750..1571
                     /locus_tag="VF453_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX7688744.1"
                     /translation="MTLPIASRRPSLSSRLAPAVIALAAAALLAGCETEAEKNAATPV
                     DKLYAEAMDDVDSGNYDHAIKELTGIEGKAAGSILAQQAQLELGYLYWKTGDKVQALA
                     TVDRFIKLHPSSPALDYALYLRGVVNFNDDLGFLGRFMGQNLSERDQAAARESYQSFK
                     QLVEQFPNSKYSADARMRMDFTVNMLAEYEVRVAAYYMRRTAYVAAANRAKEAVLEFP
                     QTASTPEALHIMVEAYDKLGLTQLRDDAQRVLDKNFPASTYALEKHKQKKWWELW"
     gene            complement(1774..1998)
                     /locus_tag="VF453_13620"
     CDS             complement(1774..1998)
                     /locus_tag="VF453_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688745.1"
                     /translation="MRTGASGDHGRGGRATRRPGDAERRAHGLDISRAVGNIGEAIET
                     VGRAIETVGRVVENLGRAIRNTGAGIPECK"
     gene            complement(2011..>3039)
                     /locus_tag="VF453_13625"
     CDS             complement(2011..>3039)
                     /locus_tag="VF453_13625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7688746.1"
                     /translation="EGLLPPALEALAAHCAALVETLEPMQDIGPDFTRLRGRADTLRQ
                     MAARFQGAAAEGQVRWADLTTHQARLVESPLDIGGPLGAQREAAPRAWIFASATLGDD
                     DQLSWFTEPAGLGDARKLRVDSPFDYEANSCVYVPPRFAAPADAHHAESVGRAAAHCA
                     AALRGRTFVLTTTLRAMRAAAQALEAELGKLGVGLEILVQGTLPKRAMLERFARGQSV
                     LVGSQSFWEGIDVPGDALQCVLIDKLPFPPPNDPLIEARVKRLQAQGRDAFNDCFVAE
                     AAVSLKQGAGRLIRTVTDRGLLVICDSRLHSARYGPRLLSALPPMRQLQDGGQALAWL
                     RELALSHD"
BASE COUNT          412 a         1161 c         1049 g          417 t
ORIGIN      
        1 ccgccggcca cgaccgccat gcgcacgcgc acgcgcgggt cgcggccgat cggggcctcg
       61 atcgtgtcct cgtcctgcgc gaggcggccg tgcgcgagcg cgaggtagcg ccggctcacg
      121 gcgcgcgccg cgatgtcgcg caccagcccc gtcaccgcct cgagcgagcg cccgaccacc
      181 atcagccccg aggtgtcctt gtcgagccgg tgcacgatgc ccgcacgcgg caacgcggcg
      241 gacgccgggt cgtgcgcgag caggccgttg agcagcgtgc ccgaccagtt gccggccgcc
      301 ggatgcacga ccagccccgc cggcttgtcg aggacgagca cgtgctcgtc ctcgtgcacg
      361 atcgcgagcg ggatcggctc cgcgcggaac gcgagctcgt gggccggccg caccagctcg
      421 acccgcacct gctgcccggc cttcacgcgg gtggaggcgg tcgtcgcggg acggccgtcc
      481 agcgccacgc ggccgtccgc gatcagcccc tgcaggtggg cgcgcgagaa ttccggcgcg
      541 agcgtggtca gcaccttgtc gaggcgttcc ccatggcgct ccaggccgat cgaggcgatg
      601 cgcacctcgg ccgcgtcgtg gtcgggctcg gcctcgtcgg gtcgggcgtc cggaccgtcg
      661 tcggcgccgt ccggcggccc catataattc gcagcttgca aagcgtttcc gttccttctc
      721 gagagactgc gccgcggcgc gaccagccca tgacccttcc gatcgcctcc cgccgcccgt
      781 ccctgtcctc gcgcctggcg ccggccgtga tcgccctcgc cgcggccgcg ctgctcgccg
      841 gctgcgagac cgaggccgag aagaacgcgg cgacgccggt ggacaagctc tacgcggagg
      901 cgatggacga cgtcgactcg ggcaactacg accacgccat caaggagctc accggcatcg
      961 agggcaaggc cgcgggctcc attctcgccc agcaggcgca gctggagctg ggctacctgt
     1021 actggaagac cggcgacaag gtgcaggcgc tcgcgaccgt cgaccgcttc atcaagctgc
     1081 atccgtcgag cccggcgctc gactacgcgc tgtacctgcg cggcgtcgtc aacttcaacg
     1141 acgacctcgg cttcctcggc cgcttcatgg gccagaacct gtccgagcgc gaccaggccg
     1201 cggcgcgcga gtcctaccag tcgttcaagc agctcgtcga gcagttcccg aactccaagt
     1261 actccgccga cgcccgcatg cgcatggact tcacggtcaa catgctggcc gagtacgagg
     1321 tgcgcgtcgc cgcctactac atgcgccgca ccgcctacgt ggcggccgcc aaccgcgcca
     1381 aggaggccgt cctcgagttc ccgcagaccg cctcgacgcc cgaggccctg cacatcatgg
     1441 tcgaggccta cgacaagctc ggcctgaccc agctgcgcga cgacgcccag cgcgtcctcg
     1501 acaagaactt ccccgccagc acctacgcgc tggagaagca caagcagaag aagtggtggg
     1561 agctctggtg agcgcctgag gccgcgcgcg cgagcgcggc cgcccgcgat ggattccgga
     1621 acatcggcgc cggcctccgg aacatcgggc ctgcatttcc gcaatgcaac gccgccccga
     1681 ttcggggcgg ctttccattt cgcgacaccg gctccggtgt ctccgattcc aacgtcggcg
     1741 ctcgcgagcc cggatcgccg ccgcctgccc gcttcatttg cattccggga tgccggcgcc
     1801 ggtgtttcgg attgcccggc cgaggttttc gacgacccgg ccgacggttt caatggcgcg
     1861 gccgacggtc tcgatcgctt cgccgatgtt tccgaccgcc cggctgatgt ccagaccgtg
     1921 ggcccggcgt tccgcatcgc ctggccggcg cgtcgcacgg ccgccgcggc cgtggtcgcc
     1981 gcttgcgccc gtccgcactt ccgcgccgcg tcaatcgtgg ctgagcgcga gctcccgcag
     2041 ccaggcgagg gcctggccgc cgtcctgcaa ctggcgcatc ggcggcagcg ccgagagcag
     2101 gcgcgggccg tagcgcgcgc tgtgcaggcg cgagtcgcag atcacgagca gcccgcggtc
     2161 ggtcaccgtc cggatcaagc gccccgcccc ctgcttcagc gacaccgccg cctcggcgac
     2221 gaagcagtcg ttgaacgcgt cgcggccctg ggcctgcagc cgcttgacgc gcgcctcgat
     2281 cagcgggtcg ttggggggcg ggaacggcag cttgtcgatc agcacgcact gcagcgcatc
     2341 gccgggcacg tcgatgcctt cccagaagct ctgcgagccc accagcacgc tctggccgcg
     2401 cgcgaagcgc tccagcatgg cgcgcttggg cagcgtgccc tgcacgagga tctcgagccc
     2461 gacgcccagc ttgccgagct cggcttccag cgcctgcgcc gcggcgcgca tcgcacgcag
     2521 cgtcgtcgtc agcacgaagg tgcgcccgcg cagcgccgcg gcgcaatggg cggcggcgcg
     2581 gccgacgctc tcggcatggt gcgcgtcggc cggcgcggcg aagcgcggcg ggacgtagac
     2641 gcaggagttc gcctcgtagt cgaacgggct gtccacgcgc agcttgcgcg cgtcgccgag
     2701 tcccgccggc tcggtgaacc agctgagctg gtcgtcgtcg ccgagcgtcg ccgaggcgaa
     2761 gatccaggcc cgcggcgccg cctcgcgctg cgcgccgagc gggccgccga tgtccagcgg
     2821 cgattcgacc aggcgcgcct ggtgcgtcgt caggtcggcc cagcgcacct gcccctcggc
     2881 cgctgcgccc tggaagcgcg cggccatctg gcgcagcgtg tcggcgcgcc cccgcaggcg
     2941 ggtgaagtcg gggccgatgt cctgcatcgg ctccagcgtc tcgaccagcg ccgcgcaatg
     3001 cgccgccagc gcctcgagcg ccggcggcag caggccctc
//
LOCUS       DASVLN010000567         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_405641, whole genome shotgun sequence.
ACCESSION   DASVLN010000567 DASVLN010000000
VERSION     DASVLN010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405641"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VF453_13630"
     CDS             complement(<1..777)
                     /locus_tag="VF453_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7688747.1"
                     /translation="MDMPSDPAPAWLVLDQVSFGYGRGPDVNRGLSLALGPGITGLLG
                     PNGAGKSTLMRMLATLARPRSGRILWHGDDIAREPDALRATLGYLPQDFGVYPALSAR
                     EFLGFLAAVKGLPARATAGRVDDCLAQVGLADAADRPLAGFSGGMRQRVGIAQALLND
                     PRLLVVDEPTVGLDPGERLRFRHLLTELAGERLVLLSTHIVSDVEASAAAIAVMKDGR
                     LLFHGTPGALVDAARGRAWDWTIPPAQLAAARRAFVVSQSL"
     gene            1002..1652
                     /locus_tag="VF453_13635"
     CDS             1002..1652
                     /locus_tag="VF453_13635"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7688748.1"
                     /translation="MSSRKSGPIDYNVLPELLRPLAERSEIRRFRKGTLVLDEGDRGE
                     HLYIVLAGELRAFTGTDTGRHVSYGLYGPGDYLGEMGLDGGPRMASVEALTDATCALV
                     ERATLLKYIGERPEFAFELMAKIIQRARVASLAASQYALNDAYGRLKRWIDAVAQAQF
                     DGTRIVREPLADVDLPQRLGCSPEMAGSILRDLEHAAIVSLDDGYLRVLRELPSHW"
     gene            complement(1661..3304)
                     /gene="ilvA"
                     /locus_tag="VF453_13640"
     CDS             complement(1661..3304)
                     /gene="ilvA"
                     /locus_tag="VF453_13640"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEX7688749.1"
                     /translation="MAAARTPRGTSSRTRTKQAADIRIAPPAPAAPLSRAAQAIAGEY
                     LRRILTARVYDVAHETPLEPAKNLSRRLGNQVLLKREDTQPVHSFKLRGAYNKMINLA
                     PAALARGVICASAGNHAQGVALGARRLGVRAVIVMPVTTPQVKVDAVAGFGGEVVLHG
                     DSYTDAYGRAVEIQKEQGLTFVHPFDDPDVIAGQGTVAMEILRQHQGPIDAVFVAIGG
                     GGLISGVAAYIKALRPEIKVIGVHTVDSDAMARSVRAGKRVTLPDVGLFADGTAVKLV
                     GEETFRVARALVDDYVLVDTDAACAAIKDVFQDTRSILEPSGALGVAAVKQYVETHKL
                     KGKTFVAVTCGANMNFDRLRFVAERAEFGEQREALFAVTIPEERGSFRRFCALIGPPG
                     AQPRQVTEFNYRISDAKVAHVFVGLTIARRDEAEKIARHFERHRFPTVNLTGDELAKE
                     HVRHMVGGRSEFARDERLFRFEFPERPGALMKFLDSMPPDWNISLFHYRNQGADYGRI
                     LVGLVVPPSDSKALARFLDTLHYPWVEETDNPVYRLFLR"
     gene            3758..4183
                     /locus_tag="VF453_13645"
     CDS             3758..4183
                     /locus_tag="VF453_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7688750.1"
                     /translation="MECTINWLPTSGMAFSAETGSGHLLTMDGAPDGGGRNLAPRPME
                     TLLAGAGGCTAYDVVLILKRGRHAVTGCQVKASAERAAEDPKVFTKLHLHFTVTGRGI
                     PAAAVERAIAMSHDKYCSATVMLGKTAEVTTSFELAEPA"
BASE COUNT          620 a         1486 c         1552 g          595 t
ORIGIN      
        1 cagcgactgg ctgaccacga aggcccggcg cgcggccgcg agctgcgcgg gcgggatcgt
       61 ccagtcccag gcgcgccctc gtgcggcgtc gacgagcgcg cccggcgtgc cgtggaacag
      121 caggcggccg tccttcatca cggcgatcgc ggcggcgctg gcctcgacgt cggagacgat
      181 gtgcgtcgac agcagcacca ggcgctcgcc cgcgagctcg gtcagcaggt ggcgaaagcg
      241 caggcgctcg ccggggtcca ggccgacggt cggctcatcg accaccagca gccgcgggtc
      301 gttgagcagc gcctgcgcga tgcccacgcg ctggcgcatg ccgcccgaga agccggccag
      361 cggccggtcg gcggcgtcgg cgagcccgac ctgggcgagg cagtcgtcga cgcgcccggc
      421 cgtcgcgcgt gccggcaggc ccttgacggc ggcgaggaag ccgaggaact cgcgggcgct
      481 gagcgccggg tagaccccga agtcctgcgg caggtagccg agcgtcgcgc gcagcgcgtc
      541 gggttcgcgg gcgatgtcgt cgccgtgcca gaggatgcgc ccggagcgcg gccgcgcgag
      601 cgtcgccagc atgcgcatca gcgtcgactt gccggcgccg ttggggccga gcagccccgt
      661 gatccccggc ccgagcgcga gcgagagccc gcggttgacg tccgggccac ggccgtagcc
      721 gaacgagacc tggtcgagca cgagccaggc gggggcgggg tcggagggca tgtccatgcg
      781 acgggtgggg cgaggtggcg gtggcggcat cctcgcggcc ggcgccgcgc gtcgccagcg
      841 ggctgcgacg agccgcgcgc gccgcccgac ggacgggcat ttgccgcgga cgagccgccc
      901 gcacgtgcac gtccggatcg cgaaaccggc tatgctggaa gaccgcaagg cacgacctcc
      961 ccgaccgcat cccgccgccg gcgccgcgcc ggcttccaga catgtcgtca cgcaaatccg
     1021 gtccgatcga ctacaacgtc ctgccggaac tcctgcgccc gctggccgag cgcagcgaga
     1081 tccggcgctt ccgcaagggc acgctggtgc tggacgaggg cgaccgcggc gagcacctct
     1141 acatcgtgct cgccggcgag ttgcgcgcct tcaccggtac cgacaccggc cgccacgtca
     1201 gctacggcct ctacggcccg ggcgactacc tcggcgagat gggcctggac ggcggcccgc
     1261 gcatggccag cgtcgaggcg ctgacggacg cgacctgcgc gctggtcgag cgcgcgacgc
     1321 tgctgaagta catcggcgag cgccccgagt tcgcgttcga gctcatggcc aagatcatcc
     1381 agcgcgcgcg cgtggcctcg ctcgcggcca gccagtacgc gctcaacgac gcctacggcc
     1441 gcctcaagcg ctggatcgac gcggtcgcgc aggcgcagtt cgacggcact cgcatcgtgc
     1501 gcgagcccct ggccgacgtc gacctgccgc agcgcctggg ctgctcgccc gagatggccg
     1561 gcagcatcct gcgcgacctc gagcacgcgg cgatcgtctc gttggacgac ggctacctgc
     1621 gcgtcctgcg cgagctgccc agccactggt gagcgcaccc tcagcgcagg aacaggcggt
     1681 agaccgggtt gtcggtctcc tcgacccacg ggtagtgcaa cgtgtcgagg aaccgggcca
     1741 gcgccttgct gtcgctcggc ggcaccacga ggccgaccag gatgcggccg tagtcggcgc
     1801 cctggttgcg gtagtggaac aggctgatgt tccagtccgg cggcatcgag tcgaggaact
     1861 tcatcagcgc gcccgggcgc tccgggaact cgaagcggaa caggcgctcg tcgcgcgcga
     1921 actcgctgcg gccgccgacc atgtggcgca cgtgctcctt cgcgagctcg tcgccggtga
     1981 ggttgacggt cgggaagcgg tggcgctcga agtggcgcgc gatcttctcg gcctcgtccc
     2041 ggcgcgcgat cgtgagtccg acgaagacgt gcgcgacctt cgcgtccgag atgcggtagt
     2101 tgaactccgt cacctggcgc ggctgcgcgc ccggcggccc gatcagcgcg cagaagcggc
     2161 ggaagctgcc gcgctcctcg gggatcgtga ccgcgaacag ggcctcgcgc tgctcgccga
     2221 actcggcgcg ctcggcgacg aagcgcaggc ggtcgaagtt catgttcgcg ccgcaggtga
     2281 cggcgacgaa ggtcttgccc ttgagcttgt gcgtctcgac gtactgcttg accgccgcca
     2341 cgcccagcgc gcccgagggc tcgaggatgg aacgcgtgtc ctggaacacg tccttgatcg
     2401 cggcgcaggc ggcgtcggtg tcgaccagca cgtagtcgtc gaccagcgca cgcgcgacgc
     2461 ggaaggtctc ctcgcccacc agcttgacgg cggtgccgtc ggcgaacagg ccgacgtcgg
     2521 gcagcgtgac gcgcttgccc gcgcgcacgg agcgcgccat cgcgtcggaa tcgacggtgt
     2581 gcacgccgat caccttgatc tcgggccgca gcgccttgat gtaggccgcc acgccggaga
     2641 tcagcccgcc gccgccgatc gcgacgaaca ccgcgtcgat cggcccctgg tgctggcgca
     2701 ggatctccat cgcgacggtg ccctggccgg cgatgacgtc ggggtcgtcg aacggatgga
     2761 cgaaggtcag gccctgctcc ttctggatct ctacggcgcg gccataggcg tcggtgtagc
     2821 tatcgccatg cagcacgacc tcgccgccga agccggcgac ggcgtcgacc ttgacctgcg
     2881 gggtggtcac cggcatcacg atgaccgccc gcacgcccag acggcgcgcg cccagcgcca
     2941 cgccctgggc gtggttgccg gcggaggcgc agatcacgcc gcgcgccagc gccgcgggcg
     3001 cgaggttgat catcttgttg tacgcgccgc gcagcttgaa gctgtgcacc ggctgcgtgt
     3061 cctcgcgctt gagcagcacc tggttgccca gacggcgcga gaggttcttc gcgggctcga
     3121 gcggggtctc gtgcgcgacg tcgtagacac gggcggtcag gatgcggcgc aggtattcgc
     3181 cggcgatcgc ctgcgcggcg cgcgacagcg gcgcggcagg ggcgggcggc gcgattcgaa
     3241 tgtccgccgc ctgcttcgtc cgggtccggg aagaggttcc acgcggggtg cgtgcggcgg
     3301 ccatggtgct ccttgtcgaa gggtgagtca gagccgccgg atcatacggc ccggctgctc
     3361 cgccggattc ttattgcgcg aatgccggcc acgtgggcag cgaacggcag cgcgcaaaga
     3421 aaaaggcccg gagcttcgca gcttcgggcc tgaatccacc agttttgagg aggtggagga
     3481 gacaacttgc ggtcggtcgg aaaggtgttt ccacctctgt cgcgaatgcg tttccgactg
     3541 acgatggact cagtctatcc gagcctttgt tgcactgcaa tatctctttt tggcatcgtg
     3601 tagggagggg cctccaatgc gcgcgggagg tgcagaaaac agccgtttcg caccagaccg
     3661 gggcgactgc gcgttgtccc ggcgcgcgcc gggccgggcc gggcgttgca caatcgacac
     3721 gacgccttcg atcccctctt gatcccggac ggacaccatg gaatgcacga tcaactggct
     3781 gcccacgagc ggcatggcct tctccgccga gaccggcagc ggccacctgc tcacgatgga
     3841 cggcgcgccc gacggcggcg gccgcaacct cgcgccgcgt ccgatggaga cgctgctggc
     3901 cggtgccggc ggctgcacgg cctacgacgt ggtgctgatc ctgaagcgcg gccggcatgc
     3961 cgtcaccggc tgccaggtga aggcgagcgc cgagcgcgcc gccgaggatc ccaaggtgtt
     4021 cacgaagctg cacctgcatt tcacggtcac cggccggggc atccccgcgg cggcggtcga
     4081 gcgcgcgatc gcgatgtcgc atgacaagta ctgctcggcg accgtcatgc tcggcaagac
     4141 cgcggaggtc acgacgagct tcgaactcgc cgagccggcc tgaggccgcc cgccggtcaa
     4201 gtcggcccgc tcacacgtgg tgggccgtcg tggtcatcac gcgcgcggcc gcg
//
LOCUS       DASVLN010000568         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_30028, whole genome shotgun sequence.
ACCESSION   DASVLN010000568 DASVLN010000000
VERSION     DASVLN010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30028"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF453_13650"
     CDS             <1..292
                     /locus_tag="VF453_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688751.1"
                     /translation="ARARDEALARAQRDEPARFDDFARRIAALEQRLDALIPRVDALA
                     REQQGALQEIAVRQLQAQQVRLASYEAQARFAVAQLYDRATDPKEGGTHAKP"
     gene            279..>2121
                     /locus_tag="VF453_13655"
     CDS             279..>2121
                     /locus_tag="VF453_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7688752.1"
                     /translation="MRSPDFRLARIAAAMAAAGALSACSLFHSKASGTPDDAPTLKTL
                     LNREAAVAPDPGIPADEDKAIAAYRAVLAVSPDERQRAEALRRLGDLQMASADEANAA
                     APTPSGTPDYGAAIKQYRDYLAQYPNGADNDRVLYQLARAQEQGGHLEDALAVLDQLV
                     AKYPRTPYIAEAQFRRGELLFSLRQYAKAEAAYGAVLAEDDGNPYRERALYMQGWSVF
                     KQGRLEDALNSFFGVLDQKIAGIEDDDLDQSDRLSRADRELVDDTLRVTSISLANLKG
                     ADSIPGYVTNDTRRSYSFRVYRSLAALYVKQDRPKDAADTFAAFARLNPLDAQAPAMQ
                     AQAIAIDQQAGFDQLALAAKKDYVAQYGLEGEFRQANPEGWQKAQPLVKTALAELAQR
                     YHASAQKTKSAADQAEAIKWYRAWLAEFHDDAEAPEYDFLLSELLYESGHYADAATEY
                     ERTAYEYPPHAHAADAGYSALLAHAAAQKGVTGEALAPLQRAGVDSELRFAAAFPADA
                     RTPAVLVNAADTLYKLHDNEHAAAVAQQVLALDPPATADQRRVAGTIVAHTAFEAGRF
                     AEAEKAYQQVLALAPANDPSRGELTERLAASIYKQGEQARAAGDARAA"
BASE COUNT          332 a          819 c          736 g          234 t
ORIGIN      
        1 agcccgtgcc cgcgacgagg cgctcgcccg cgcgcagcgc gacgagcccg cccggttcga
       61 cgacttcgcg cgccgcatcg cggcgctcga gcagcgcctg gacgcactca tcccgcgcgt
      121 cgacgcgctc gcccgcgagc agcagggcgc gctgcaggag atcgccgtgc gccagctgca
      181 ggcgcagcag gtgcggctcg cgagctacga ggcgcaggcg cgcttcgccg tcgcccagct
      241 ctacgatcgc gcgaccgatc cgaaggaagg gggcacgcat gcgaagccct gacttccgcc
      301 tcgcccgcat cgccgcggcg atggcggccg cgggcgcgct gtcggcgtgc tcgctgttcc
      361 actcgaaggc gagcggcacg ccagacgacg cgccgacgct gaagacgctg ctcaatcgcg
      421 aggccgcggt cgcccccgat cccggcatcc cggccgacga ggacaaggcg atcgccgcct
      481 accgcgccgt gctggccgtc tcgcccgacg agcgccagcg cgccgaggcg ctgcgacggc
      541 tcggcgacct gcagatggcc agcgccgacg aggccaatgc cgccgcgccc actcccagcg
      601 ggacgccgga ctacggcgcc gcgatcaagc agtaccgcga ctacctcgcg cagtatccga
      661 acggcgccga caacgaccgc gtgctctacc agctcgcgcg cgcccaggag cagggcggcc
      721 acctcgagga cgcgctcgcc gtgctcgacc agctggtcgc gaagtacccg cgcacgccct
      781 acatcgccga ggcccagttc cggcgcggcg agctgctgtt ctcgctgcgc cagtacgcca
      841 aggccgaggc cgcgtacggc gcggtgctgg ccgaggacga cggcaacccg taccgcgagc
      901 gcgcgctcta catgcaaggc tggtcggtgt tcaagcaggg ccggctggag gacgcgctga
      961 actcgttctt cggcgtcctc gaccagaaga tcgccggcat cgaggacgac gacctcgacc
     1021 agtccgaccg cctgtcgcgc gccgaccgcg agctcgtcga cgacaccctg cgcgtgacca
     1081 gcatctcgct cgcgaacctg aaaggcgcgg actccatccc gggctacgtc acgaacgaca
     1141 cgcggcgcag ctactcgttc cgcgtctacc gcagcctggc cgcgctgtac gtgaagcagg
     1201 atcgcccgaa ggacgccgcc gacaccttcg ccgccttcgc gcgcctgaac ccgctcgacg
     1261 cccaggcgcc ggcgatgcag gcgcaggcga tcgcgatcga ccagcaggcc ggcttcgacc
     1321 agctcgcgct cgccgcgaag aaggactacg tcgcgcagta cggcctcgag ggcgagttcc
     1381 gccaggccaa tcccgagggc tggcagaagg cccagccgct ggtcaagacg gcgctggccg
     1441 agcttgcgca gcgctaccac gcgagcgcgc agaagacgaa gtcggctgcc gaccaggccg
     1501 aggcgatcaa gtggtaccgc gcctggctcg ccgagttcca cgacgacgcc gaggcgccgg
     1561 agtacgactt cctgctgtcc gagctgctgt acgagagcgg gcattacgcc gacgccgcga
     1621 ccgagtacga gcggacggcc tacgagtacc cgccgcatgc gcacgcggcg gacgcgggct
     1681 actcggcgct cctcgcgcat gcggcggcgc aaaagggcgt gacgggcgag gcgctcgcgc
     1741 cgctgcagcg cgccggcgtc gacagcgagc tgcgcttcgc cgccgccttc ccggccgacg
     1801 cgcgcacgcc cgcggtgctc gtgaacgcgg ccgacacgct ctacaagctg cacgacaacg
     1861 agcacgccgc cgcggtcgcg cagcaggtgc tcgcgctcga cccgccggcc accgcggacc
     1921 agcgccgcgt cgccggcacc atcgtcgcgc acacggcgtt cgaggccggg cgcttcgccg
     1981 aagcggagaa ggcgtaccag caggtgctcg cgctcgcgcc cgcgaacgac ccgtcgcgtg
     2041 gcgagctcac cgagcgcctg gccgcctcga tctacaagca gggcgagcag gcgcgcgcgg
     2101 ctggcgacgc ccgcgccgcg g
//
LOCUS       DASVLN010000569         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_138951, whole genome shotgun sequence.
ACCESSION   DASVLN010000569 DASVLN010000000
VERSION     DASVLN010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138951"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1072)
                     /locus_tag="VF453_13660"
     CDS             complement(101..1072)
                     /locus_tag="VF453_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX7688753.1"
                     /translation="MSDATTPAIPGREDAILVAGARGMVGSALVRRLTAGGYARVLAP
                     PRAELDLLDAAATRAYLAHHRPAYVFIAAAKVGGIQANDRLRAQFLHENLAIADHLVH
                     GAHLAGVRRLMFLGSSCIYPRLAPQPIREDALLTGPLEPTNEPYAIAKIAGVKLCEAY
                     NAQYGHRYVSAMPTNLYGPNDNYDLASSHVLPALIRKAHEASRRGASELVVWGTGTPR
                     REFLYVDDLADACVHLMERGYDGPLVNVGTGEDVTIRELAETVMAVVGFPGRIAFDAS
                     KPDGTPRKLLDVSRLHALGWRHRVGLREGIARAYRDFLARAAGEARA"
     gene            complement(1065..2183)
                     /gene="gmd"
                     /locus_tag="VF453_13665"
     CDS             complement(1065..2183)
                     /gene="gmd"
                     /locus_tag="VF453_13665"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7688754.1"
                     /translation="MAKKIALVTGVTGQDGAYLAEFLLGKGYEVHGVKRRASSFNTSR
                     IDHLYQDPHVAGRDFILHYGDLTDSTNLLRIVQKVQPDEIYNLAAQSHVAVSFEEPEY
                     TANADALGPLRLLEAIRILGREKLTRFYQASTSELYGLVQRTPQDEATPFHPRSPYAA
                     AKLYAYWITVNYREAYGIHACNGILFNHESPLRGETFVTRKITRAVARIALGQQDCLW
                     LGNLGARRDWGHARDYVEAMWRMVQLPAPEDFVIATGVQASVRDFVRLAAARLGIALR
                     FEGEGGEELGIVDAVERDRASCRPGDVVVRVDPRYYRPTEVDALLGDAGKAREKLGWA
                     PRTSLDELVAEMVDADHDAARRDALVRGAGFRTYDQHE"
BASE COUNT          301 a          827 c          880 g          337 t
ORIGIN      
        1 tcgcgctcat cgaggcgatg gccgagagcg tggagtgggt cgagctcgac ccgtccgacg
       61 cgcgccgcgc cgagttcggc gtcgacgggc cgcgctgggt ctaggcgcgg gcctcgcccg
      121 cggcgcgggc caggaagtcc cggtaggcgc gcgcgatgcc ttcgcgcagg cccacgcggt
      181 ggcgccagcc gagcgcatgc aggcgcgaga cgtccagcag cttgcgcggc gtgccgtccg
      241 gcttcgaggc gtcgaacgcg atgcgacccg ggaacccgac cacggccatc acggtctcgg
      301 cgagctcgcg gatcgtcacg tcctccccgg tgccgacatt gaccagcggg ccgtcgtagc
      361 cgcgctccat gaggtgcacg caggcgtcgg cgaggtcgtc cacgtagagg aactcgcgcc
      421 ggggcgtgcc ggtgccccag acgacgagct cggacgcgcc gcgccggctc gcctcgtgcg
      481 ccttgcggat cagcgccggc agcacgtggc tgctcgcgag gtcgtagttg tcgttcgggc
      541 cgtagaggtt cgtcggcatc gcgctcacat accggtggcc gtattgcgcg ttgtaggcct
      601 cgcacagctt cacgccggcg atcttggcga tcgcgtaggg ctcgttggtc ggctccagcg
      661 gcccggtcag cagcgcgtcc tcgcggatcg gctgcggcgc gagcctcggg tagatgcagc
      721 tggagccgag gaacatcagc cgccgcacgc ccgccaggtg cgcgccgtgg accaggtggt
      781 cggcgatcgc caggttctcg tgcaggaact gcgcgcgcag gcggtcgttg gcctggatgc
      841 cgccgacctt ggccgccgcg atgaagacgt aggccggccg gtggtgcgcg aggtaggcgc
      901 gcgtcgccgc ggcgtcgagc aggtcgagct ccgcgcgcgg gggcgcgagc acgcgcgcgt
      961 agccgcccgc cgtcagccgc cgcaccagcg ccgagcccac catgccgcgc gcgccggcga
     1021 cgaggatcgc gtcctcgcgc ccggggatcg ccggcgtcgt cgcgtcactc atgctggtcg
     1081 taggtccgaa agccggcgcc gcgcacgagg gcatcgcggc gggcggcgtc atggtcggcg
     1141 tcgaccatct cggcgacgag ctcgtccagc gaggtgcgcg gcgcccagcc gagcttctcg
     1201 cgtgccttgc ccgcgtcgcc gagcagcgcg tcgacctcgg tcgggcggta gtagcgcgga
     1261 tcgacgcgga cgaccacgtc gcccggacgg cagctcgcgc gatcacgctc gacggcgtcg
     1321 acgatgccga gctcctcgcc gccctcgccc tcgaagcgca gcgcgatccc cagtcgcgcc
     1381 gcggccaggc gcacgaagtc gcgcacgctc gcctgcacgc cggtggcgat gacgaagtcc
     1441 tcgggcgcgg gcagctgcac catccgccac atcgcctcga cgtagtcgcg ggcgtgcccc
     1501 cagtcgcgcc gcgcgccgag gttgccgagc cacaggcagt cctgctggcc gagcgcgatg
     1561 cgggcgacgg cgcgcgtgat cttgcgcgtc acgaaggtct cgccgcgcaa cgggctctcg
     1621 tggttgaaca ggatgccgtt gcaggcgtgg atgccgtagg cctcgcggta gttgacggtg
     1681 atccagtacg catagagctt ggccgcggcg tacgggctgc gcgggtggaa cggcgtcgcc
     1741 tcgtcctgcg gcgtgcgctg caccaggccg tacagctcgg acgtggacgc ctggtagaag
     1801 cgcgtcagct tctcgcggcc gaggatgcgg atcgcctcca gcagccgcag cgggcccagg
     1861 gcgtcggcgt tggcggtgta ctcgggctcc tcgaagctca cggcgacgtg gctctgcgcg
     1921 gcgaggttgt agatctcgtc gggctgcacc ttctgcacga tgcgcagcag gttcgtggaa
     1981 tcggtgaggt cgccgtagtg caggatgaaa tcgcgtcccg cgacgtgggg atcctggtac
     2041 aggtggtcga tgcggctcgt gttgaacgag gacgcccggc gcttgacgcc atgcacctcg
     2101 tagcccttgc cgagcaggaa ctcggcgagg taggcgccgt cctgcccggt gacgccggtc
     2161 accaacgcga tcttcttggc catcgggcga ttatgagcga ggcgcccgtc cggacggcgc
     2221 gccccacgag ctccggcgcc ctccccctgc acgcgccgcg ccgccgcgca aatcgcccct
     2281 tcggggggcg cgccggcggg cgccgacgcg gcatgatggc gggcatgcgc atgcccgtcc
     2341 cgttc
//
LOCUS       DASVLN010000570         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_515586, whole genome shotgun sequence.
ACCESSION   DASVLN010000570 DASVLN010000000
VERSION     DASVLN010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515586"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VF453_13670"
     CDS             <1..225
                     /locus_tag="VF453_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688755.1"
                     /translation="AAASLASGVGLERPVDPPTVAPAADLDAAWEQVRALACAQDDDH
                     VIKLVHAMVDQDAQRPSTTWLAAARAAVRA"
     gene            327..596
                     /locus_tag="VF453_13675"
     CDS             327..596
                     /locus_tag="VF453_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX7688756.1"
                     /translation="MPHVAFTSQLQRFTETPELDTPAATLREALDAAFALNPRLRHYV
                     LDEQGHLRQHVAVFIDGRRVADRTGLGDALGPDDKVYVLQALTGG"
     gene            622..1686
                     /locus_tag="VF453_13680"
     CDS             622..1686
                     /locus_tag="VF453_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006480576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX7688757.1"
                     /translation="MADRAWVATRKGLIELRRRAGAWAVERTSFLGDPVSMVLPPDGD
                     GRMFAALSLGHFGVKLHASDDAGASWREVAAPAFPPQPEGAPGPAWKVTMVWAMERAG
                     ATLLVGTLPGGLFRSDDGAAGWRLDEALWNRPERLGWNGGGYDQPGIHSISPLADGTV
                     LIGVSCGGAWITRDAGASWSQTAHGMRAEYMPPERAYDPEVQDPHAIARCAAAPDVLW
                     CQHHNGIFRSVDGGAHWGELQAPLSSFGFAVAAHPADPRTAWFVPAVKDQCRVPVDRA
                     LAVNRTRDGGASFETLRAGLPQRDCYDLVYRHGLAVDDEGTGLMMASTTGGVWASDDG
                     GDHWDALPARLPPVYALRFG"
BASE COUNT          210 a          652 c          644 g          205 t
ORIGIN      
        1 gcggcggcga gcctcgcctc gggcgtcggc ctcgagcgcc ccgtcgaccc gcccaccgtc
       61 gcgccggccg ccgacctcga cgccgcctgg gagcaggtgc gtgccctcgc ctgcgcgcag
      121 gacgacgacc atgtcatcaa gctcgtgcac gcgatggtcg accaggacgc gcagaggccg
      181 tcgacgacat ggctggccgc ggcgcgggcg gcggtgcggg cctgaagaac cggtgggcgg
      241 cgggccggcc gggcgtcctc ggctcggtcc tcggctcggg ataggcccga ttgccgcccc
      301 gccgcgcgcg cgcatcatgc gcccgcatgc cccacgtcgc gttcacctcg cagctgcagc
      361 gcttcaccga gacgccggag ctcgacacgc ccgccgcgac gctgcgcgag gcgctagacg
      421 ccgccttcgc gctcaacccg cgcctgcgcc actacgtgct cgacgagcag ggccacctgc
      481 gccagcacgt ggccgtcttc atcgacggcc gccgcgtggc cgaccgcacg ggcctgggcg
      541 acgcgctggg gccggacgac aaggtgtatg tgctgcaggc cctgaccggc ggctgacgaa
      601 cgcaaggacg aggagcaccg catggcggat cgtgcatggg tggccacccg caagggcctg
      661 atcgaactgc gccggcgtgc cggcgcctgg gcggtggagc ggaccagctt cctgggcgac
      721 ccggtgagca tggtgctgcc gcccgatggc gacggacgca tgttcgccgc gctgagcctg
      781 gggcacttcg gcgtcaagct gcacgcgtcg gacgacgcgg gcgcgagctg gcgcgaggtc
      841 gcggcgccgg ccttcccgcc gcagcccgag ggggcgcccg ggccggcatg gaaggtgacg
      901 atggtgtggg cgatggagcg cgcgggcgcc acgctgctcg tcggcacgct gcccggcggg
      961 ctgttccgct ccgacgacgg cgcggccggc tggcggctgg acgaggcgct gtggaaccgc
     1021 ccggaacgcc tcggctggaa cggcggcggc tacgaccagc cgggcatcca ctcgatcagc
     1081 ccgctcgccg acggcaccgt gctgatcggc gtgagctgcg gcggcgcctg gatcacgcgc
     1141 gacgccggcg cgtcctggtc ccagaccgcg cacggcatgc gcgccgagta catgccgccc
     1201 gagcgcgcct acgaccccga ggtgcaggat ccgcacgcca tcgcgcgctg cgccgccgcg
     1261 cccgacgtgc tctggtgcca gcaccacaac ggcatcttcc gctcggtcga cggcggcgcg
     1321 cactggggcg agctgcaggc gccgctgtcg tccttcggct tcgcggtggc cgcgcatccc
     1381 gccgacccgc gcaccgcgtg gttcgtcccg gcggtcaagg accagtgccg cgtgcccgtc
     1441 gaccgcgcgc tcgcggtcaa ccgcacgcgc gacggcggcg cgagcttcga gaccctgcgc
     1501 gccggcctgc cccagcgcga ttgctacgac ctcgtctatc gccacggcct cgcggtcgat
     1561 gacgagggca ccggcctcat gatggccagc accaccggcg gcgtctgggc gagcgacgac
     1621 ggcggcgacc actgggacgc gctgccggcg cggctgccgc cggtctacgc gctgcggttc
     1681 ggctgaaggg ccggggacgc cccgtcggcc g
//
LOCUS       DASVLN010000571         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_30075, whole genome shotgun sequence.
ACCESSION   DASVLN010000571 DASVLN010000000
VERSION     DASVLN010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30075"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2803)
                     /locus_tag="VF453_13685"
     CDS             complement(<1..2803)
                     /locus_tag="VF453_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7688758.1"
                     /translation="MDAARFAPLDVFTCPLAGLRQIEASAGTGKTWNICGLYLRLLLE
                     RGMTVQQVLVVTFTKAATAELRERIRQRIVDTLAYLRPAHDDSQPPGDGFVERLVQAA
                     ADHHDLTDAEMVARLERALASFDEAAIHTIHGFCQRAIGDAPFSTRMPLVQEQLEDDA
                     AHVAEAANDFWRRHVAADGFDAALAAHLLSRGDDPARFARLLKRHLGKPLAREIWPED
                     VEDVDLVASAGGESSAAAAEGAPPVGERLAALHADARAAWMDGREAIAAMLREALELE
                     AFKANMVDADKLAAALQGWDDRLGDDDALAWLEVDDRQLKRIELLGSAKLASATKKKF
                     APPGHAFFEHAQALLDERAQAADRLERARLALLRRLLREGAQAQRAAKRRARVVAFDD
                     MLFNLYERLASPESPGLAASLRARYPAALVDEFQDTDPLQFAVFRAIYAGDAARGTDA
                     AAPVFFVGDPKQAIYSFRNADLHTYLAAGAQAVGHYSLAANQRSTAALIRALNALFTA
                     NPRAFMLDGLDYQPVECGAKPRTALADASGGDTAALQVWTLPDDPATGEPVLKGDADR
                     LAAEATAAEIARLLTAARQGVLVLGEGATARPLAAGDVAVLVRSHAQGSRMRHALAAL
                     GVGSVELSQDSVLRSADAAELARLLDAVLEPARERTLKAALATEAMGVDAEGLVALAQ
                     DEAALLATVQRFLDYRETWARRGVGVMLRQWMAAEDVAARLLARPDGERRLTNLLHLF
                     ELLHEAGETRPAPDALLQWFRAQRQAPDAGEAAQLRLESDQDLVQIVTVHKSKGLEYP
                     FVFCPFLWGAEAAPGDGLEGAAYHDDHGRAVIDYGIRLRDRSAQDAVKAAMQRERAAE
                     DLRLVYVALTRAVHRCVLVAGCYSKRTANGGVSATGSTHGVLNWLVAGAGLAPADWFA
                     HKLGAPAMAAAWQALAARAP"
     gene            complement(2803..>2998)
                     /locus_tag="VF453_13690"
     CDS             complement(2803..>2998)
                     /locus_tag="VF453_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688759.1"
                     /translation="GNLAQAERSFRPSRNNAFAEGADDAVRLALRGRPDPFRGEAARD
                     FAAIAEAVYGPLRAHLEEAA"
BASE COUNT          360 a         1098 c         1150 g          390 t
ORIGIN      
        1 cgggcgcccg cgcggccagc gcctgccagg ccgcggccat cgcgggcgcg ccgagcttgt
       61 gggcgaacca gtcggccggc gcgagccccg cgcccgcgac cagccagttg aggacgccgt
      121 gcgtgcttcc ggtcgccgac acgccgccgt tggccgtccg cttcgaatag cagcccgcca
      181 ccagcacgca ccggtgcacc gcccgggtga gcgcgacgta gaccagccgc aggtcctcgg
      241 ccgcgcgctc gcgctgcatc gcggccttga ccgcatcctg cgccgacctg tcgcgcaggc
      301 ggatgccgta gtcgatcacc gcccggccgt ggtcgtcgtg gtacgccgcg ccttccagcc
      361 cgtcgcccgg cgcggcctcg gccccccaga ggaacgggca gaacacgaac gggtactcga
      421 ggcccttgga cttgtgcacc gtgacgatct gcacgagatc ctggtcggat tccaggcgca
      481 gctgcgccgc ctcgccggcg tcgggcgcct ggcgctgggc gcggaaccac tgcagcagcg
      541 cgtcgggcgc gggtcgcgtc tcgccggcct cgtgcagcag ctcgaacagg tgcagcaggt
      601 tggtcaggcg gcgctcgccg tcgggccgcg cgagcagccg cgcggcgacg tcctcggcgg
      661 ccatccactg gcgcagcatc acgccgacgc cgcgccgcgc ccaggtctct cggtagtcga
      721 ggaagcgctg caccgtcgcg agcagcgccg cctcgtcctg cgcgagcgcc accaggccct
      781 cggcgtccac gcccatcgcc tcggtcgcga gcgcggcctt gagcgtgcgt tcgcgcgcgg
      841 gctcgaggac ggcgtccagc aggcgcgcga gctcggcggc gtcggcgctg cgcagcacgc
      901 tgtcctgcga gagctcgacg ctgcccacgc ccagcgccgc gagcgcgtgc cgcatccgcg
      961 acccctgggc gtggctgcgg acgagcaccg cgacgtctcc ggcggcgagc ggacgcgcgg
     1021 tcgcgccttc tccgagcacg agcacgcctt gccgcgccgc cgtgagcagg cgcgcgatct
     1081 cggcggcggt cgcctcggcg gcgaggcggt cggcgtcgcc cttgagcacc ggctcgcccg
     1141 tggccggatc gtcgggcagc gtccagacct gcagcgccgc ggtgtcgcca ccgctcgcgt
     1201 cggccagcgc ggtgcgcggc ttcgcaccgc actcgaccgg ctggtagtcc aggccgtcca
     1261 gcatgaaggc gcgcgggttg gccgtgaaca gggcgttgag cgcgcggatc agtgccgccg
     1321 tcgagcgctg gttggccgcg agcgagtagt ggccgaccgc ctgcgcgccg gccgcgaggt
     1381 aggtgtgcag gtcggcgttg cggaagctgt agatcgcctg cttggggtcg ccgacgaaga
     1441 agaccggcgc ggcggcgtcc gtgccgcggg ccgcgtcgcc ggcgtagatc gcgcggaaga
     1501 ccgcgaactg aagcgggtcg gtgtcctgga actcgtcgac cagcgcggcg gggtagcgcg
     1561 cgcgcagcga ggccgcgagg ccgggcgact cggggctcgc gaggcgctcg tacaggttga
     1621 acagcatgtc gtcgaacgcg acgacgcgcg cgcgccgctt ggccgcgcgc tgggcctgcg
     1681 ccccctcgcg cagcaggcgg cgcagcagcg cgaggcgggc gcgctcgagg cggtcggccg
     1741 cctgggcgcg ttcatcgagc agcgcctgtg cgtgttcgaa gaaggcgtgt cccggaggcg
     1801 cgaacttctt cttggtcgcg gacgcgagct tcgccgagcc gagcagctcg atgcgcttca
     1861 gctgccggtc gtccacttcc agccacgcga gcgcgtcgtc gtcgccgagc cggtcgtccc
     1921 agccctgcaa tgcggcggcc agcttgtccg cgtcgaccat gttggccttg aaggcctcga
     1981 gctcgagcgc ctcgcgcagc atcgcggcga tcgcctcgcg tccgtccatc cacgcggcgc
     2041 gcgcgtcggc gtgcagcgcg gcgaggcgtt cgccgaccgg cggcgctcct tcggcggcgg
     2101 cggcggagga ctcgccgccc gccgacgcga cgaggtcgac gtcctcgaca tcctccggcc
     2161 agatctcgcg tgccaggggc ttgccgaggt gacgcttgag caggcgcgcg aagcgggcgg
     2221 gatcgtcgcc gcggctcagc agatgcgccg caagcgccgc gtcgaagccg tcggccgcga
     2281 cgtggcggcg ccagaagtcg ttcgcggcct cggcgacgtg ggcggcgtcg tcctcgagct
     2341 gctcctgcac gagcggcatc cgggtggaga acggcgcatc gccgatcgcg cgctggcaga
     2401 agccgtggat cgtatggatc gcggcctcgt cgaagctcgc gagcgcgcgc tccaggcggg
     2461 cgaccatctc ggcgtcggtg aggtcgtggt ggtcggcagc ggcctgcacc agccgctcca
     2521 cgaagccgtc gcccgggggc tggctgtcgt cgtgcgccgg gcgcaggtag gccagcgtgt
     2581 cgacgatgcg ctggcggatg cgctcgcgca gctccgcggt cgcggccttg gtgaaggtga
     2641 cgacgagcac ctgctgcacc gtcatgccgc gctcgagcag caggcgcagg tacaagccgc
     2701 agatgttcca ggtcttgccg gtgccggccg aggcctcgat ctgccgcagc ccggcgagcg
     2761 ggcaggtgaa gacgtccagc ggggcgaagc gggcggcgtc catcaggcgg cctcctccag
     2821 gtgcgcccgc agcggcccgt agaccgcctc ggcgatggcc gcgaagtcgc gcgccgcctc
     2881 gccgcggaac ggatcgggcc ggccgcgcag ggcgagccgc acggcgtcgt cggcgccctc
     2941 ggcgaacgcg ttgttgcggc tcggccggaa gctgcgctcg gcctgcgcga ggttgccg
//
LOCUS       DASVLN010000572         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_406064, whole genome shotgun sequence.
ACCESSION   DASVLN010000572 DASVLN010000000
VERSION     DASVLN010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406064"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..494)
                     /locus_tag="VF453_13695"
     CDS             complement(114..494)
                     /locus_tag="VF453_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7688760.1"
                     /translation="MQFGYTILYVDDVPAALAHYEAAFGLATRFLHESGTYGELETGA
                     TALAFAARELLAQQGKATASPVRGQPSFEIALTTDDVPAALARAIAAGATLVQEAKAM
                     PWGQVVGYVDDRHGFRVEVCTPMG"
     gene            713..2350
                     /locus_tag="VF453_13700"
     CDS             713..2350
                     /locus_tag="VF453_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX7688761.1"
                     /translation="MPVLSTHLNARSEDFKANAAAMRRLVDDLNAKLARIAQGGGEDA
                     RKKHLARGKLLPRERVERLLDPGTPFLEIAPLAALGMYPEKGGQDAAPGAGMIAGIGR
                     VAGVECLIVCNDATVKGGTYYPMTVKKHLRAQEIAQQNRLPCIYLVDSGGANLPNQDE
                     VFPDRDHFGRIFYNQANLSAAGIPQLAVVMGSCTAGGAYVPAMSDQTVIVKEQGTIFL
                     GGPPLVKAATGEVVSAEDLGGGDVHTRLSGVADHLAEDDMHALALARGIVGTLNWRKD
                     PAIALREPVPPKLPASELHGIIPLDAEGKPARKPFDVREVIARIVDGSEFDEFKARYG
                     ATLVCGFAWIEGMPVGIVANNGILFAESADKGAHFIELCCQRKVPLVFLQNITGFMVG
                     RKYENEGIARAGAKMVTAVACAQVPKFTVIIGGSFGAGNYGMCGRAYSPRFLWMWPNA
                     RISVMGGEQAASVLATVRRDGIEAKGGQWSAEEEEAFKAPIRDQYELQGHPYFASARL
                     WDDGVIDPADTRRVLALGLSASLNAPIPDTKFGVFRM"
     gene            2448..>3005
                     /locus_tag="VF453_13705"
     CDS             2448..>3005
                     /locus_tag="VF453_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688762.1"
                     /translation="MKLARIRPSAARRAAAALLAVAATTALLPSAQARNVRRMESLAD
                     TLHASKAHEILGDLDLRFGGSTAQGTDIVRHDVVVTGTGATRLDEVGKDDRIRPSDAE
                     LCVRAFEDAITQLAFSARAAHATGIVGVVSDYEDNVFDDPDHYECHAGSLKAYVTLRA
                     QLARGTTAPVPVVSPPPAPVVPPAPA"
BASE COUNT          438 a         1105 c         1065 g          397 t
ORIGIN      
        1 cacatgacgc gcgacctgcg ccgctggttc ggcgcgccgc ccggcgcgat gcgcgcgacg
       61 ccgcagcggt gggcggcgct gcacgagagc gggttcggct gagccggggc gcctcacccc
      121 atcggcgtgc agacctcgac ccggaacccg tgccggtcgt cgacgtagcc gaccacttgg
      181 ccccagggca tcgccttcgc ctcctggacg agcgtcgcgc ccgccgcgat ggcgcgcgcg
      241 agggcggcag gcacgtcgtc ggtggtgagc gcgatctcga agctgggctg gccccgcacc
      301 ggcgaggcgg tcgccttgcc ctgctgcgcc agcagctcgc gagcggcgaa ggcgagcgcg
      361 gtggcgccgg tctcgagctc gccgtaggtg ccggactcgt gcaggaagcg cgtggccagg
      421 ccgaaggcgg cctcgtagtg ggcgagggcg gcggggacgt cgtcgacgta gaggatggtg
      481 tagccgaact gcatggcgga tgtcggggtg atcggtcgat ggcgcagtgt cgcgccggcc
      541 ggcgcacgcg tcttgaagaa aaccgtcgtc cgcggcgacc gtccgggact acgatgcgcc
      601 gacgccgtcg cggctcgaat tgacgtttac gtaaacgtca atcgataatc ccgcgtccac
      661 cgcgccgcgc tcgccgccgg ccgcgccacc cgaacgacga tcctcgccgc ccatgcccgt
      721 cctctcgacc cacctgaacg cccgctcgga agacttcaag gccaatgccg cggcgatgcg
      781 ccggctggtc gacgacctca acgccaagct cgcgcgcatc gcgcaggggg gcggcgagga
      841 cgcccgcaag aagcacctgg cgcgcggcaa gctgctgccg cgcgagcgcg tggagcggct
      901 gctggacccg ggcacgccct tcctggagat cgcgcccctg gccgcgctcg gcatgtatcc
      961 ggaaaagggc ggccaggacg cggcgccggg cgccggcatg atcgcgggca tcgggcgcgt
     1021 cgcgggcgtc gagtgcctga tcgtctgcaa cgacgccacg gtcaagggcg gcacctacta
     1081 cccgatgacc gtcaagaagc acctgcgcgc gcaggagatc gcgcagcaga accgcctgcc
     1141 ctgcatctac ctggtcgact cgggcggcgc caacctgccc aaccaggacg aggtcttccc
     1201 cgaccgcgac cacttcgggc gcatcttcta caaccaggcc aacctcagcg ccgcgggcat
     1261 cccgcagctg gcggtggtga tgggctcctg caccgccggc ggcgcctacg tgcccgcgat
     1321 gagcgaccag accgtcatcg tcaaggagca gggcaccatc ttcctgggcg gcccgccgct
     1381 cgtgaaggcc gccacgggcg aggtcgtgag cgccgaggac ctgggcggcg gcgacgtcca
     1441 tacgcgcctg tcgggcgtgg ccgaccacct ggccgaggac gacatgcacg cgctcgcgct
     1501 ggcgcgcggc atcgtcggca cgctgaactg gcgcaaggat cccgccatcg cgctgcgcga
     1561 gccggtgccg ccgaagctgc cggcctcgga gctgcacggc atcatcccgc tggatgccga
     1621 gggcaagccc gcgcgcaagc ccttcgacgt gcgcgaggtg atcgcccgca tcgtcgacgg
     1681 ctccgagttc gacgagttca aggcgcgcta cggcgcgacg ctggtgtgcg gcttcgcgtg
     1741 gatcgagggc atgccggtgg gcatcgtcgc caacaacggc atcctcttcg ccgagagtgc
     1801 cgacaagggc gcgcacttca tcgagctgtg ctgccagcgc aaggtgccgc tcgtcttcct
     1861 gcagaacatc accggcttca tggtcggccg caagtacgag aacgaaggca tcgcccgcgc
     1921 cggcgcgaag atggtgaccg cggtcgcctg cgcgcaggtg cccaagttca cggtgatcat
     1981 cggcggctcg ttcggcgccg gcaactacgg catgtgcggc cgcgcctaca gcccgcgctt
     2041 cctgtggatg tggccgaacg cgcgcatcag cgtgatgggc ggcgagcagg ccgcgagcgt
     2101 gctggccacc gtgcgccgcg acggcatcga ggccaagggc ggccagtggt cggccgagga
     2161 ggaggaggcc ttcaaggcgc ccatccgcga ccagtacgag ctgcagggcc acccgtactt
     2221 cgccagcgcc cggctctggg acgacggcgt gatcgacccg gccgacacgc gccgcgtcct
     2281 cgccctgggc ctgagcgcca gcctgaacgc gccgatcccg gacacgaagt tcggcgtgtt
     2341 ccgcatgtga gccgcgtgtg aacgcccacc cacgtcgccg gccttcggat tgccctgctc
     2401 tgcggttggc cgccgtcgcg gccgccactg gagaaaaggg cacgatcatg aaactcgctc
     2461 gcatccgtcc gtccgccgcc cgccgggccg ccgccgcgct gctggccgtc gcggcgacga
     2521 cggcgctcct cccgtccgcg caggcgcgca acgtgcgccg catggagtcg ctcgccgaca
     2581 cgctgcacgc ctcgaaggcg cacgagatcc tcggcgacct cgacctgcgc ttcggtggtt
     2641 cgacggcgca gggcacggac atcgtccgcc acgacgtcgt cgtcaccggc accggcgcga
     2701 cgcgcctgga cgaggtcggc aaggacgatc gcatccgccc gtccgacgcc gagctgtgcg
     2761 tgcgcgcctt cgaggacgcg atcacgcagc tcgcgttctc ggcgcgcgcc gcgcatgcga
     2821 cgggcatcgt gggcgtcgtc tcggactacg aggacaacgt cttcgacgat cccgaccact
     2881 acgaatgcca cgccggctcg ctcaaggcct acgtgacgct gcgcgcccag ctcgcgcgcg
     2941 gcaccacggc ccccgtgccg gtggtctcgc cgccgcccgc gccggtcgtc ccgccggcgc
     3001 cggcg
//
LOCUS       DASVLN010000573         3741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_30347, whole genome shotgun sequence.
ACCESSION   DASVLN010000573 DASVLN010000000
VERSION     DASVLN010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30347"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VF453_13710"
     CDS             <1..977
                     /locus_tag="VF453_13710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HEX7688763.1"
                     /translation="RVRVAVFVDAPPEVAFEVFTAQIDQWWRRGLKFRAGGASPSVLH
                     LEPRLGGRLFETVAADGGEAHVVQTGEVTAWSPPHALAIAWRGTNFAPGERTTIAVRF
                     EPRGAGTQVTLEHSGFAALRPDHPVRHGEPVAVFVRRTAMWWADQFASLRERVGGFAI
                     VSTADTAARIDVLAAAPWMTIARAELGVRAGPPGASNPRITAYHAHTNIAGYDDRASW
                     CSSFADWALRRTGLAGTGSALARSWLEWGRPLDAPRPGCIVVLWRDDPASWKGHVGFF
                     VRIAGEEIVLLGGNQREQEVCEHAYPLASVLGYRWPLDPRWPDGVPAR"
     gene            1098..1550
                     /locus_tag="VF453_13715"
     CDS             1098..1550
                     /locus_tag="VF453_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7688764.1"
                     /translation="MTSSTDPSAGIRRLFDRYRDRVLAKDAAGLLALYRDDVRVFDFW
                     DAWSHEGLPAWGASVGQWLGSLGDETVRVAFDDVRIEAGDRLASASFVTTYAAVSATG
                     EVLRSMQNRGTWSLSREEDGDWRIFHEHTSAPIRSADLKGVLQRPSAG"
     gene            complement(1555..2355)
                     /locus_tag="VF453_13720"
     CDS             complement(1555..2355)
                     /locus_tag="VF453_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX7688765.1"
                     /translation="MTGPQRIVCLTEETTEWLYLLGEERRIVGISGYTVRPRRARDEK
                     PRVSAFLDGKIDRIVALRPDLVIGFSDMQAALADKLIRAGLNVFITNQRSVAEILATM
                     RLVAGMVGAVDRAEAWIARCLARHAEIAQAAAAWPRRPRVYFEEWDEPMISAIRWVSE
                     LVAIAGGDDVFAELGLHAGGKDRIVHDLAEPPRRAPDVVVGSWCGKKFRPERVAARAG
                     WSEVPAVARGELHEVKSCDILQPGPAALTDGLEQLHRIFAAWAAKPGA"
     gene            2465..>3741
                     /locus_tag="VF453_13725"
     CDS             2465..>3741
                     /locus_tag="VF453_13725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7688766.1"
                     /translation="MSPVPSRHRAPHPFLFLVLFVPFGAVPGFLTVALAYELSKKGID
                     TGDIAIVVGLSYICLILKWLWAPLVDTLFTRKIWYVGSTLVCALGFWLAGRAAMGEHP
                     SLALISAALTVSNFTATLLAMAVESLMAATVPDDQRGRAGGWSQAGNLGGQGLGGGLS
                     LWLIQSQGLTDAQSGAALGVLCALCCLALFRVDDTRPLLKVDGHVEAAGHLGVGANLK
                     AIGGDLWQLLCSRVGILALLICLLPIGSGAAQNLWSPIASEWHASAETVAFVTGAMSG
                     LISALGCLAGGWLCDRLDRRTAYCLFGFLLALAAIGMALCPRTSAQYIFWTSAYAFVV
                     GLCYAAYCAVVLEAIGQTAAATKFSFLSAVSNTPIMVMTFVDGAAQSRWGTNGMLWAD
                     AVCGVIGIAVFALVAGGTRRAAVARASAAEPASA"
BASE COUNT          439 a         1371 c         1383 g          548 t
ORIGIN      
        1 accgcgtgcg cgtcgccgtc ttcgtcgacg cgccgcccga ggtcgcgttc gaggtcttca
       61 ccgcgcagat cgaccagtgg tggcgccgcg gcctgaagtt ccgtgcgggc ggcgcgagcc
      121 cgagcgtgct gcacctcgag ccgcgcctgg gcggacgcct gttcgagacc gtcgcggccg
      181 acggcggcga ggcgcacgtc gtgcagaccg gcgaggtgac cgcctggtcg ccgccgcacg
      241 cgctcgcgat cgcatggcgg ggcaccaact tcgcgcccgg cgagcgcacg acgatcgccg
      301 tgcgcttcga gccgcgcggc gcgggcaccc aggtgacgct cgagcacagc ggcttcgcgg
      361 cgctgcgacc cgaccatccc gtgcgccacg gcgagccggt cgcggtcttc gtgcggcgca
      421 ccgcgatgtg gtgggcggac cagttcgcct cgctgcgcga acgcgtcggc ggcttcgcca
      481 tcgtctcgac ggccgatacg gccgcccgta tcgacgtcct cgctgcggcg ccgtggatga
      541 cgatcgcccg tgccgagctg ggcgtgcgcg ccggcccgcc gggcgcgagc aatccgcgca
      601 tcaccgcgta ccacgcgcac accaacatcg ccggctacga cgaccgcgcg agctggtgct
      661 cgtcgttcgc cgactgggcg ctgcgccgca ccggcctcgc gggcaccggc tcggcgctcg
      721 cgcgcagctg gctggaatgg ggccgcccgc tcgacgcgcc gcgccccggc tgcatcgtcg
      781 tgctgtggcg cgacgacccc gcgagctgga agggccacgt cggcttcttc gtgcgcatcg
      841 cgggcgagga gatcgtgctg ctgggcggca accagcgcga gcaggaggtt tgcgagcatg
      901 cctatccgct cgcgagcgtg ctgggctacc gctggccgct ggatccgcgc tggccggacg
      961 gcgtcccggc ccggtagcgg ccaccggggg acggccggcg accgcccgcc gccctgcgcc
     1021 tgctcgccat ggccggggtc gacgcgcgcg cgcggggctg gcagcatcgg cctttcgcca
     1081 cccacccggg cctcgccgtg acgtcatcga ccgatccttc cgccggcatc cgccgcctct
     1141 tcgaccgcta ccgcgatcgc gtgctcgcca aggatgccgc cggcctgctc gcgctgtacc
     1201 gcgacgacgt gcgcgtgttc gacttctggg acgcctggtc gcacgagggc ctgccggcct
     1261 ggggcgcgag cgtcgggcag tggctcgggt cgctcggcga cgagacggtg cgcgtcgcct
     1321 tcgacgacgt gcgcatcgag gccggcgacc gcctcgcgtc cgcgagcttc gtgacgacct
     1381 atgccgcggt ctcggcgacg ggcgaagtgc tgcgctcgat gcagaaccgc ggcacctgga
     1441 gcctgtcgcg cgaggaggac ggcgactggc gcatcttcca cgagcacacg tccgcgccga
     1501 tccgctcggc cgacctgaaa ggcgtgctcc agcgtccgtc cgcgggctga cgcgtcacgc
     1561 gcccggcttc gccgcccagg ccgcgaagat gcggtgcagc tgctcgagcc cgtcggtcag
     1621 tgccgccggc ccgggctgca ggatgtcgca agacttgacc tcgtgcagct cgccgcgcgc
     1681 gaccgcgggc acctcggacc agccggcgcg cgcggccacg cgctcggggc ggaacttctt
     1741 gccgcaccag ctgcccacga cgacgtcggg cgcgcggcgc ggcggctcgg cgaggtcgtg
     1801 gacgatgcgg tccttgccgc ccgcgtgcag gcccagctcg gcgaagacgt cgtcgccgcc
     1861 ggcgatcgcc accagctccg agacccagcg gatcgcgctg atcatgggct cgtcccactc
     1921 ctcgaaatag acgcgcggcc ggcgcggcca cgcggcggcc gcctgcgcga tctcggcgtg
     1981 gcgcgcgagg catcgcgcga tccaggcctc ggcacggtcg accgcgccga ccatgccggc
     2041 gacgagccgc atcgtcgcca ggatctcggc cacgctgcgc tggttcgtga tgaacacgtt
     2101 caggccggcg cggatcagct tgtcggcgag cgcggcctgc atgtccgaga agccgatcac
     2161 gaggtcgggc cggagcgcga cgatgcggtc gatcttgccg tccaggaacg ccgagacgcg
     2221 cggcttctcg tcgcgcgccc ggcgcggccg caccgtgtag cccgagatgc cgacgatgcg
     2281 gcgctcctcg ccgaggaggt acagccactc ggtggtctcc tcggtgaggc agacgatgcg
     2341 ctggggtccg gtcatgcggg gattgtgccc ggcggtcgga gggcccgccg atcgtcgagg
     2401 ctcgccttgc agcacgccgg ggctgtcccg ggggacaatc gcgcaccctt tcgagaaagg
     2461 cgccatgagc cccgttccct cgcgccaccg cgcgccgcat ccgttcctgt tcctcgtgct
     2521 gttcgtgccc ttcggcgcgg tgccggggtt cctcacggtc gcgctggcct acgagctcag
     2581 caagaagggc atcgacactg gcgacatcgc catcgtggtg ggcctgagct acatctgcct
     2641 gatcctgaag tggctctggg cgccgctggt cgacacgctg ttcacgcgca agatctggta
     2701 cgtcggctcg acgctggtat gcgcgctcgg cttctggctc gcgggccgcg cggcgatggg
     2761 cgagcatccg tcgctcgcgc tcatcagcgc cgcgctgacg gtgagcaact tcacggcgac
     2821 gctgctcgcg atggccgtcg agagcctgat ggccgccacc gtccccgacg accagcgcgg
     2881 ccgcgcgggc ggctggtcgc aggccggcaa cctcggcggc cagggcctgg gcggcggcct
     2941 gagcctgtgg ctgatccaga gccagggcct cacggacgcg cagtccggcg cggcgctggg
     3001 cgtgctgtgc gcgctctgct gcctcgcgct gttccgggtg gacgacaccc ggccgctgct
     3061 gaaggtcgac ggccatgtcg aggccgccgg ccatctgggc gtgggcgcga acctgaaggc
     3121 gatcggcggc gacctgtggc agctgctgtg ctcgcgcgtc ggcatcctcg cgctgctgat
     3181 ctgcctgctg ccgatcggca gcggcgcggc gcagaacctg tggtcgccga tcgcgtcgga
     3241 atggcacgcc tcggccgaga ccgtcgcctt cgtcaccggg gcgatgagcg gcctcatctc
     3301 cgcgctcggc tgcctggcgg ggggctggct gtgcgaccgc ctggaccgcc gcacggccta
     3361 ctgcctgttc ggcttcctgc tggcgctggc cgccatcggc atggcgctgt gcccgcgcac
     3421 gtccgcgcag tacatcttct ggacctcggc ctacgccttc gtggtgggcc tgtgctacgc
     3481 ggcgtactgc gcggtggtgc tggaggcgat cggccagacg gccgcggcga ccaagttcag
     3541 cttcctgtcg gcggtctcga acacgcccat catggtgatg accttcgtcg acggcgcggc
     3601 gcagagtcgc tggggcacga acggcatgct gtgggcggac gccgtctgcg gcgtcatcgg
     3661 catcgcggtg ttcgcgctgg tggcgggcgg gacgcgccgc gccgccgtgg caagggccag
     3721 cgcggcggag ccggcgagcg c
//
LOCUS       DASVLN010000574        28081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_516094, whole genome shotgun sequence.
ACCESSION   DASVLN010000574 DASVLN010000000
VERSION     DASVLN010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 28081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28081
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516094"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..725
                     /locus_tag="VF453_13730"
     CDS             102..725
                     /locus_tag="VF453_13730"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX7688767.1"
                     /translation="MAQLFDIHPDNPQLRLLRQAAQILHDGGLAAVPTDSSYAFVCHL
                     DDKDAVERLRRVRGVDDKHHLTLLCRDLSELATYARVDNKQYRLIKQATPGPYTFILD
                     ATKEVPRRVSHPSRRTIGLRVPDHQVLQALLAEFGEPLIGTTLIPPGETEPLNDTDEI
                     RARFEKSLQAIVCAGACPREPTTVIDLSGPQPTVVREGRGEVAALGL"
     gene            complement(744..1310)
                     /locus_tag="VF453_13735"
     CDS             complement(744..1310)
                     /locus_tag="VF453_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688768.1"
                     /translation="MPHPARLPLRALFTLLMAACAAAHGQARLADDACRDVGTGTPER
                     PVAVLVDGTHRLRLGPIELGVVSPPAVDAAAASAPAPGLAAAVSDTGFHVLVAPVSAQ
                     PDSVPDAPRAVQLIEEPSACPGSRSFRVVLAWGSTTPAQAAAARTPGRFVLPLPDFTF
                     ATRDEHDHGSRALYAFDARVIGEVAAAP"
     gene            complement(1378..1857)
                     /locus_tag="VF453_13740"
     CDS             complement(1378..1857)
                     /locus_tag="VF453_13740"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEX7688769.1"
                     /translation="MHSFRLVGLPSDPFQALFDLDDDALRARAIRRCVADEPFGFPCR
                     ISLEDAAVGDELLLLSHEHLALDSPYRAGGPIFVRRGARRASLAPGVLPPYVTRRLVS
                     ARAYDAGGMMLAADVCEGEAAQAATIARLFEDPAVDFLHLHNARRGCYSCRVERVAA"
     gene            complement(1970..3319)
                     /gene="phoR"
                     /locus_tag="VF453_13745"
     CDS             complement(1970..3319)
                     /gene="phoR"
                     /locus_tag="VF453_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEX7688770.1"
                     /translation="MGNSLGRALLYVVLALLGAAIGALVGHALDASVVGTAVGAALAV
                     GARSLVDELRAHRLMRWLRSDHERQAPRAPGLWGEAAYRIERALRQRDQALRGEQERF
                     AEFMSAIEASPNGVLLLDSADQIEWLNPMAATHFGLDPARDRLQRVTNLVRSPSFVAY
                     LQSGEFDDPIVFSAPGGEATLSVIVSRYGEGMRLVLSQDITERERTEAMRRDFVANVS
                     HEIRTPLTVLAGFVETISTLPLTDVERERVLGLMTQQTSRMTTLVEDLLTLAKLEGSP
                     RPACDRWTPVSRVLMQVESEARGLSAGRHGFRFTIAQDDEIAGNDSEWTSAIANLVSN
                     AVRYTPAGGSIAVTWSRRSDGSAEIEVKDTGPGIAREHIGRLTERFYRVDGSRSRETG
                     GTGLGLSIVKHVVQRHGGELDIQSELGRGSTFRIVVPAARVRPHAPAQPVAEESAAR"
     gene            complement(3330..4052)
                     /gene="phoB"
                     /locus_tag="VF453_13750"
     CDS             complement(3330..4052)
                     /gene="phoB"
                     /locus_tag="VF453_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX7688771.1"
                     /translation="MSRILVVEDESAIAELIAINLRHSGFEVTIAETADAAQGEVDRV
                     LPELVVLDWMLPGQTGLALAKQWRSVARTRDLPIIMLTARSDEADKLSGLDAGADDYL
                     TKPFSTKELLARIRAVLRRKAPEALDSAVEVSGLRLDPATRRVTRGDREVKIGPTEFR
                     LLHFFMTHTERVHSRTQLLDRVWGDHVFIEERTVDVHVKRLREALAPVQCSTLIETVR
                     GAGYRLTQQVLPAANPQAANPA"
     gene            complement(4178..4885)
                     /gene="phoU"
                     /locus_tag="VF453_13755"
     CDS             complement(4178..4885)
                     /gene="phoU"
                     /locus_tag="VF453_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX7688772.1"
                     /translation="MTDKHLSTQFDAELSEISNRVLEMGGLVESQVAQAVYALTNFSG
                     ETASLVLDREQRVNTMEVEIDRDLSAIIARRQPTARDLRLLIAISKTIGNLERIGDEA
                     TRIARTVQRLMNSGVSSRLRLPVADIAFGANLATAQLRKVLDAFARLDVGRALEVLKQ
                     DDEIDKEFDGLLRKLITYMMEDPRTISSSIDLVFVAKAIERVGDHAKNLSEQIIYIVK
                     GTDVRHHTVAAVEDAVR"
     gene            complement(4954..5739)
                     /gene="pstB"
                     /locus_tag="VF453_13760"
     CDS             complement(4954..5739)
                     /gene="pstB"
                     /locus_tag="VF453_13760"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX7688773.1"
                     /translation="MDAKVELPVGEKAKLAVRNLNFYYGAFHALKNINLDIPEGKVTA
                     FIGPSGCGKSTLLRTFNRMFALYPEQRATGEIVLDGENILESRLDVSLIRAKVGMVFQ
                     KPTPFPMSIYDNIAFGVRLFETLPRVEMDERVEWALKKAALWNDVKDKLDQSGSALSG
                     GQQQRLCIARGIAIKPEVLLLDEPCSALDPISTGKIEELIHELKSDYTVVIVTHNMQQ
                     AARCSDYTAYMYLGDLVEFGKTTDLFMKPKKKDTEDYITGRFG"
     gene            complement(5758..6627)
                     /gene="pstA"
                     /locus_tag="VF453_13765"
     CDS             complement(5758..6627)
                     /gene="pstA"
                     /locus_tag="VF453_13765"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX7688774.1"
                     /translation="MTAISALPPARMAMHNRRKRVNVIALSLAFGAMAFGVFWLVWIL
                     IETVRLGGGGLNLALFTESTPAAMSDGGGLANALYGSALMVLVATFIGTPIGIFSGVY
                     LAEYGQKSWLASTTRFINDILLSAPSIVIGLFVYELIVVPTRAFSGWAGSVALALIVI
                     PVVIRTTENMLNLVPHALREAAYALGTPKWKVISSVTLTAARSGVITGILLAIARIVG
                     ETAPLLFTALSNQFFSTSLAKPLSSVPMVIFNFAMQPYPNLQRIAWAGVFLITLGVLA
                     LNIAARVFFRNKN"
     gene            complement(6659..7678)
                     /gene="pstC"
                     /locus_tag="VF453_13770"
     CDS             complement(6659..7678)
                     /gene="pstC"
                     /locus_tag="VF453_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7688775.1"
                     /translation="MAATLPAESNLRKAKDGTQPVGRPPERRQNSPWADALFSFLAHG
                     AAWLTLAMLAGIIGSLIVGAMPALRAYGLSFLWTSVWDPVQDHYGGLAMIYGTLATSF
                     IALLIAVPVSFGIALFLTEMSPAWLKRPLGIAIELLAGVPSIVYGMWGLTVFGPILAN
                     YVQGPLQNAFGTVPVLGTLFSGPPVGIGILCAGIILAIMIIPFIASVMRDVFEVTPPM
                     LKESAYGLGATTWEVVYKVVLPFTKTGVIGGIMLGLGRALGETMAVTFVIGNQSQLSQ
                     ISLFQAANTITSVLANEFAEAGEGLHRAALLYLALILFVITFIVLALAKVLLMRLRRG
                     EGAKS"
     gene            complement(7755..8795)
                     /gene="pstS"
                     /locus_tag="VF453_13775"
     CDS             complement(7755..8795)
                     /gene="pstS"
                     /locus_tag="VF453_13775"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX7688776.1"
                     /translation="MTFHPIRLVAVAAALGATSFVAQAEDVTGAGSSFAAPIYSKWAE
                     AYAKASSNHVNYQSVGSGAGIKQIKAKTVDFGASDMPLKDDELAQSGLVQFPTCMGGV
                     VPVVHLDGVKPGQLKLTGTVLADIYAGKVTKWNSPEITALNPGVTLPDAAIATVHRAD
                     GSGTSFIFTNYLSKVNADWKAKVGEGTAVNWPANGVGGKGNEGVAQYVLQLKNSIGYV
                     EYAYAKQNKMDYVQMKNQAGAFVSPDDATFKAAAAGADWSKTFYQVLTDEPGKDSWPI
                     TGATFVLLHKTQEKPAQGAAVMKFFDWAFTNGDKAAAELDYVSLPDSVKSLIRKQWAE
                     VKDSSGKTVAFK"
     gene            complement(9007..10341)
                     /gene="glmM"
                     /locus_tag="VF453_13780"
     CDS             complement(9007..10341)
                     /gene="glmM"
                     /locus_tag="VF453_13780"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7688777.1"
                     /translation="MSRTHFGTDGIRGTVGTAPITADFMLRLGHAVGRVLRRNAPRAT
                     VLIGKDTRVSGYMIESALEAGFASAGVDVLLTGPLPTPGVAYLTRALRLDLGVVISAS
                     HNPFGDNGVKFFSASGEKLPDQWELDVEAALEEAPQWVDSAALGKARRINDAAGRYLE
                     FCKSTFASDLSLKGLRIVVDAAHGAAYQVAPSVFHELGAEVVTIGCEPDGFNINAGVG
                     ATAPQALQRAVAEHRADYGVALDGDADRLQMVDASGRCFDGDELLYAVVADRLGRGEP
                     VPGVVGTLMTNMAVEQALLGRGVPLVRAKVGDRYVLEELLGRGWQLGGENSGHLLVLD
                     RHTTGDGIISALQVLQAIRRGGRSLAELLEGVTLFPQVLINVRLAARRDWQDNAALVA
                     GKAEVERELGHDGRVLIRASGTEPVLRVMVEARDGALARRCAERLAEAAKLE"
     gene            complement(10412..11296)
                     /gene="folP"
                     /locus_tag="VF453_13785"
     CDS             complement(10412..11296)
                     /gene="folP"
                     /locus_tag="VF453_13785"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7688778.1"
                     /translation="MSSLPVQADPPAGPPAIRAWQAGRFRIALDRPRVMGIVNVTPDS
                     FSDGGRYAQADAACAHCDRLLEEGVDILDIGGESTRPGARLPDTAEQLGRVLPVLRHA
                     VTLGVPVSVDTADPAVMQAALELGVDILNDVRALQAPGALGVAAGHPSAGVCLMHMQG
                     EPGTMQVAPHYVDDDVVGEVARFLAARRDAALAAGIAADRITLDPGIGFGKTQAHNLA
                     LGRRQRELLALGHPLLLGWSRKSTLGALTGRPVGDRVAASVAAAIAAVAAGAAIVRVH
                     DVAQTVDALRVWQALSAG"
     gene            complement(11442..13352)
                     /gene="ftsH"
                     /locus_tag="VF453_13790"
     CDS             complement(11442..13352)
                     /gene="ftsH"
                     /locus_tag="VF453_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7688779.1"
                     /translation="MNNQWFSKIAIWLVIALVLFTVFKSFDHGSSQGIKESYSEFLDD
                     VHNKRISSAVITESGTTIDIVATPSDGGSKKHVVGSSLLDRGLVGDLLANGVKFEITP
                     RDEPSLLTSLLLSWAPMLLLIGVWVYFMRQMQGGGKGGAFSFGKSKARMLDEANNSIT
                     FADVAGCDEAKEEVKELVDFLRDPQKFQKLGGRIPRGVLMVGPPGTGKTLLAKAIAGE
                     AKVPFFSISGSDFVEMFVGVGAARVRDMFEQAKKSAPCIIFVDELDAVGRHRGAGLGG
                     GNDEREQTLNQMLVEMDGFETNLGVIVMAATNRPDILDPALLRPGRFDRQVYVTLPDI
                     RGREQILNVHMRKVPIGTDVNANVIARGTPGMSGADLANLVNEAALMAARRSARTVEM
                     IDFERAKDKIFMGAERRSAVIDPEELKNTAYHEAGHAFVMLKLPKHDPVHKVTIIPRG
                     RALGVAFYLPEKDNHAPDKEYMLANIAALYAGRIAEEVFMNQQTVGASNDFQRATQLA
                     RDMVMKYGMSESLGPMVYAENEGEVFLGRSVTRQTNMSEATMQKVDAEIRRILDGQYN
                     LARTLIETERDKVEAIAKALLEWETIYREQLDDIMAGREPRPPKDWTPSINRSGGSNP
                     PPLNPDGAPATA"
     gene            complement(13516..14205)
                     /locus_tag="VF453_13795"
     CDS             complement(13516..14205)
                     /locus_tag="VF453_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX7688780.1"
                     /translation="MKTNTKSKKVNKAWLNDHVHDPYVKAAQKDGYRARAAYKLQEID
                     EQFKLFRPGQLVVDLGSAPGAWSQYLRRRFAPKSAGAGGAAVGALNGRIVALDILPME
                     PIEGVDFLQGDFREDAVLAQLQALVGGQPVDVVVSDMAPNLSGVADTDAARVSHLVEL
                     AIDFAIHHLKPEGALVTKVFHGSGYSQLMEIYKANFRVVKAIKPKASRDRSAETFIVG
                     FGLKSAPAKPA"
     gene            14226..14696
                     /locus_tag="VF453_13800"
     CDS             14226..14696
                     /locus_tag="VF453_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEX7688781.1"
                     /translation="MPLIELTPAERKEKRGEAHHLDPVVMIGADGLTAPVLKEAEAAI
                     KAHGLIKVRVFSDDRAAREALLLELADKLDAAPVQHIGKLLVLWRPVEASAPAPSEDR
                     GAAPRVVKILKFGKRPGQRPELKRVRVLGNQRVTAGGQVKRAKKRVTSIKKKVD"
     gene            complement(14736..15182)
                     /locus_tag="VF453_13805"
     CDS             complement(14736..15182)
                     /locus_tag="VF453_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEX7688782.1"
                     /translation="MPLPELLRRFVPGVWLGVLAAIAGVAMPSAFAALDRAAAGQLAR
                     VVFEREAMLSLALGVLVLIAERRAGVDAHEATGASQFTSGMMLALGAIFCTVAGFYGL
                     QPLMEEARAGRATALSFGQLHLVSLGFFAAKGLMVLALAWKALRRD"
     gene            complement(15206..15682)
                     /gene="greA"
                     /locus_tag="VF453_13810"
     CDS             complement(15206..15682)
                     /gene="greA"
                     /locus_tag="VF453_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7688783.1"
                     /translation="MATIPLTRRGAEKLKEELHRLKTVERHAVIQAIAEARAQGDLSE
                     NAEYEAAKDKQGFIEGRILEIEGKLAAAQVIDPSEVDGGGRVVFGATVVLEDEESGQE
                     VTYQIVGEDEADLKEGMISVGSPIARAMIGKEAGDTAAVQAPGGVKNYEIVAVKYI"
     gene            complement(15822..19097)
                     /gene="carB"
                     /locus_tag="VF453_13815"
     CDS             complement(15822..19097)
                     /gene="carB"
                     /locus_tag="VF453_13815"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX7688784.1"
                     /translation="MPKRLDIQSILIIGAGPIIIGQACEFDYSGAQACKALREEGFKV
                     ILVNSNPATIMTDPNTADVTYIEPITWQVVEKIIAKERPDAILPTMGGQTALNCALDL
                     HRHGVLAKYGVEMIGANEHAIEKAEDRLKFKDAMTKIGLGSAKSGIAHSMEEAWAVQK
                     SIAAEIGGTGFPMVIRPSFTLGGTGGGIAYNPEEFEEICKRGIDLSPTNELLIEESLI
                     GWKEYEMEVVRDKADNCIIICSIENLDPMGIHTGDSITVAPAQTLTDKEYQLMRNASV
                     AILREIGVDTGGSNVQFSINPADGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLA
                     VGYTLDELRNDITGGATPASFEPSIDYVVTKIPRFAFEKFREADPHLTTQMKSVGEVM
                     AMGRTFQESFQKALRGLETGIDGLTERTACREADREEIVEQIANAGPERILFVGDAFR
                     IGMTLDEVHEQTAIDPWFLAQIEDLVKVEGQLRGRTLESLTAAELRWVKQKGFSDKRL
                     GTLLKSNQHDVRRARQALGVRPVYKRVDTCAAEFATQTAYMYSAYETEDGECEAAPSN
                     RKKIMVLGGGPNRIGQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRL
                     YFEPVTLEDVLEIVAIEKPHGVIVQYGGQTPLKLALDLEANGVPIIGTTPDSIDIAED
                     RERFQQLLHTLGLKQPPNRTARTEEAALQLAQEIGYPLVVRPSYVLGGRAMEIVHGDK
                     DLERYMREAVKVSEKSPVLLDRFLDDAIEVDVDCISDGADVMIGGIMEHIEQAGVHSG
                     DSACSLPPYSLSPALQDELRRQTALMARALKVVGLMNVQFAIQGEGDEAVVYVLEVNP
                     RASRTVPYVSKATGQSLAKIAARCMAGTKLADQKGLGGKPPVETVPPYFTVKEAVFPF
                     NKFPGVDPVLGPEMRSTGEVMGVGRTFAEAMLKSQLGAGSRLPTKGNVVLTVKNADKA
                     RTVKVASDLVNLGFTISATKGTAAAIAEAGIPVRLVNKVKDGRPHIADMIKAGEVQLV
                     FTTVDETRTAIADSRYIRTAAIANRVTYYTTMAGCEAATEALKHGDDITVVSLQELHA
                     ELQ"
     gene            complement(19090..20268)
                     /gene="carA"
                     /locus_tag="VF453_13820"
     CDS             complement(19090..20268)
                     /gene="carA"
                     /locus_tag="VF453_13820"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7688785.1"
                     /translation="MLPVLPPAILALADGTVFSGTSIGAPGHTVGEVVFNTALTGYQE
                     ILTDPSYCKQIVTLTYPHIGSYGVSGEDVEASKVHAAGLIIKDLPLRRSNFRASLSLD
                     EYLAREGTVAIAGIDTRKLTRLLRTNGAQNGCIVAFPAGHAVTDADRADAIARARAGE
                     NMAGLDLAQVVSCTERYEWTQTEWKLGSGYGTLQSPRFHVVAYDYGVKFNILRMLAER
                     GCRITVVPAKTPAAEVLALNPDGVFLSNGPGDPEPCDYAIAATRTIVESGLPTFGICL
                     GHQIMALAAGAKTFKMKFGHHGANHPVKENATGRVSITSQNHGFAVDPATLPANLVPT
                     HVSLFDGTLQGLARTDKPAFCFQGHPEASPGPHDIAYLFDRFTSLMAEAQSRKKSPNA
                     "
     gene            20605..21300
                     /locus_tag="VF453_13825"
     CDS             20605..21300
                     /locus_tag="VF453_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX7688786.1"
                     /translation="MERNVQRLQTLNGGNVYGFDQHRVLRNTYWLLALSMIPTVAGAW
                     LGLATGFTRMMAPGLSLVLFMVGAYGLMALVHAWRNSSKGVVAMLGFTFFMGLMLSRL
                     LGFALGFANGSALIMTAFGGTAGVFFGMATLATVVKRDLSALSRFLFVGIMIAIVASL
                     VNFFVQSTAAFLAISVAVTAISSLFILVDVKRVIDGGEDNYITAALSIYIDLYNVFQN
                     LLMLLTIFTGDRR"
     gene            complement(21367..21894)
                     /locus_tag="VF453_13830"
     CDS             complement(21367..21894)
                     /locus_tag="VF453_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688787.1"
                     /translation="MVEDVVTGWVSADDPQYGFPGATGELQSRVVRETGTGTLDFYWK
                     VDVDFASYPSYVPTSLTIAGLTLADFGTGAAFDADYRIDGLGTMAPTDAWATPTSLTW
                     DFDPSAFGPGSESYFLLLRSTATTFDENALAELGASSIATFEPAAAVPEPAPRSLLVA
                     GLAAVALLGRRLRAR"
     gene            complement(22070..22453)
                     /locus_tag="VF453_13835"
     CDS             complement(22070..22453)
                     /locus_tag="VF453_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688788.1"
                     /translation="MDDAELETLVRDASRIGTSAAHDRLLRAMRGRTVYFNATPGQGG
                     APAQVATFEAAGRRGVDAVCSRRHPDLRPSFGGMPWERALEMAAKMLALDGLFLRNAA
                     NDWIFVDRASAARLAEGPSGSPDPG"
     gene            complement(22467..22904)
                     /locus_tag="VF453_13840"
     CDS             complement(22467..22904)
                     /locus_tag="VF453_13840"
                     /inference="COORDINATES: protein motif:HMM:NF018837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEX7688789.1"
                     /translation="MTDPDIERAIADAAQANMPAARARLLERLRGKEVFFNLSPGEEM
                     RTPLVAVGPGLRALPLFSSREHPRLRQEKFGGMPWERALEMVARMPDAQGLVLSNLED
                     RWVAMDKPHILARLGEAAPPEEGAPSDVPVSRTELRRRASGTN"
     gene            complement(22901..24424)
                     /gene="rlmD"
                     /locus_tag="VF453_13845"
     CDS             complement(22901..24424)
                     /gene="rlmD"
                     /locus_tag="VF453_13845"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX7688790.1"
                     /translation="MSTDQPTAGGKKERRGRNHRRAEPAPGVTDSEWLKVLRIDLEAQ
                     GIAHNSEGKVVFIDGALPGEEARVHVVRRKKQWEQAELVALRRESAQRVKPRCPHFGL
                     HAGACGGCKMQHLGPAAQVAMKQRVLEDNLWHLGKVRPEIVLRPIEGPAWGYRLRARL
                     SVRHVAKKGTVLVGFHERKSSYVADIRECHVLPRRISDMLLPLRELVGRMQARDRLPQ
                     IEVAIGDQVTALVLRHLDPLSEHDLGELRAFGAEHAVQWWLQPKGPDTIHRIDEDGPE
                     LAYLLPEFGVRMPFRPTDFTQVNHQINTVLVGRALRLLDVQSHEDVIDWFCGLGNFTL
                     PLATQARSVLGIEGSQTLVERALANARVNGLDARTRFDARNLFELVPDDLVALGTVDK
                     WLVDPPREGAFALAKALAELKAAPRAGWTAPRRIVYVSCNPATLARDAGLLVHQAGYR
                     CVAAGAVNMFPHTAHVESMAVFDWDPDWQPRAAAPVFDEAVGGDAQDEAGEDAADTP"
     gene            complement(24506..26065)
                     /locus_tag="VF453_13850"
     CDS             complement(24506..26065)
                     /locus_tag="VF453_13850"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7688791.1"
                     /translation="MRLTNLKIGVRLALAFGTVLALLATVSIIALVRTGSMRNDMNAV
                     TGGIAVESSLADQMRLAATQQAVRVRNILMVSEPEEMAAENELLKADIKTYDTAKARL
                     ATLIGNDGDARKKTILGHIDAAEKEARDLSGQVVQMALDNHKFPARGLLGDKANPAQA
                     KWMAAVDEMVALNEKLTAAAVSGAEGAYETTRNIVLGISVVALVFASWIGLAVTRSIT
                     VPISKAVEIARRVADRDLTVRVDSSSDDETGQLLKALKAMTESLVTVVASVRNGSDSL
                     AGAATEIASGNADLSNRTERQAASIQQTASSMDELSSTVKMSASNSTHARQLSTEAAD
                     KAQQGGEAVSKVVRTMGDIASSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQG
                     RGFAVVATEVRTLAQRSATAAKEIKSLIAESVEKVDEGTHLVDVAAGTIAQIVQSVQH
                     VAGIVSEITSATVEQSAGIDLVNSAVSEMDQVTQQNAALVEQVASAADSMRSQVQRLV
                     SDVSVFRLATH"
     gene            complement(26210..26572)
                     /locus_tag="VF453_13855"
     CDS             complement(26210..26572)
                     /locus_tag="VF453_13855"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7688792.1"
                     /translation="MKPHVRSRLIRAAAAGALAALASAGALAADFKWVQDAKSCAPPE
                     YPRESLSKGEQGTTGLRILVGVDGKAIDSEIESSSHSRKLDSAAQRALMECSFKPSPA
                     DAKPVQQWVKTEFVWKLN"
     gene            complement(26883..27740)
                     /locus_tag="VF453_13860"
     CDS             complement(26883..27740)
                     /locus_tag="VF453_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7688793.1"
                     /translation="MIGCASTNQAPVEDHSRSTAGHLAPAAEPAKASAPVAPPVEAKP
                     GTYIVQPGDILSRIGLNTGQGWKDLARWNNITDPDKIEVGQVLRVVPPPGETTNTTTT
                     TTTTTFKPLPAKTDKPEPKASGVAVAGSAVALPPAASNASATTPAPLTNVATPPPAAH
                     ENDDEPNWAWPANGAVVAPFDDARSKGLSIAGKAGDPVYAAADGKVVYAGSGLRGYGN
                     LVIVKHNANYLTAYAHNQTLLVKEDQFVKRGQKIAEMGSTDTDRVQLHFEIRRQGKPV
                     DPAKLLPPR"
     gene            complement(27821..>28081)
                     /locus_tag="VF453_13865"
     CDS             complement(27821..>28081)
                     /locus_tag="VF453_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX7688794.1"
                     /translation="VRLVHGDGRHGHPPNAPYDSIVAAAGGEAIPDAWLQQLAPGGRL
                     VAPTAMPGLAGQALVIVDRTPGGFVRQVGESVTFVPLKSGIG"
BASE COUNT         4019 a         9641 c         9995 g         4426 t
ORIGIN      
        1 cggcgccggc cgccgcggtg gacatcgccc ccgagtgacg cccgccgcgc gacgcgtcct
       61 gcactgacgc cagggtcgcg cagcggctgc cacaatggcc catggcccag ctcttcgaca
      121 tccacccgga caacccgcaa ctgcggctgc tgcgccaggc cgcacagatc ctgcatgacg
      181 gcgggctcgc ggcagtgccg acggattcga gctacgcctt cgtctgccac ctcgacgaca
      241 aggacgccgt ggagcgcctg cgccgcgtgc gcggcgtcga cgacaagcac cacctgacgc
      301 tgctgtgccg cgacctctcg gagctcgcga cctacgcgcg cgtggacaac aagcagtacc
      361 gcctcatcaa gcaggccacg cccggcccgt acaccttcat cctcgacgcg accaaggagg
      421 tgccgcgccg cgtgtcgcat ccgtcgcgcc ggacgatcgg cctgcgcgtg cccgaccacc
      481 aggtgctgca ggcgctgctg gccgagttcg gcgagccgct gatcgggacg accctgatcc
      541 cgcccggcga gaccgagccg ctgaacgaca ccgacgagat ccgcgcccgc ttcgagaaga
      601 gcctgcaggc catcgtctgc gcgggcgcct gcccgcgcga gccgacgacc gtcatcgacc
      661 tcagcgggcc gcagccgacc gtcgtgcgcg aaggccgtgg cgaggtcgcg gccctcggcc
      721 tgtagccggc cggctgtccg ccctcaaggc gcggcggcga cctcgccgat cacgcgtgcg
      781 tcgaacgcgt agagcgcgcg cgagccgtgg tcgtgctcgt cgcgggtcgc gaaggtgaag
      841 tccggcagcg gcagcacgaa gcggccgggc gtgcgcgccg cggcggcctg cgcgggcgtc
      901 gtcgagcccc aggccagcac cacgcggaag ctgcggctgc ccgggcaggc cgacggctcc
      961 tcgatcaact gcacggcacg cggcgcgtcc gggacgctgt cgggctgtgc gctcacgggc
     1021 gcgacgagga cgtggaagcc ggtatcggac accgcggccg cgaggcccgg cgcgggcgcc
     1081 gaggcggctg ccgcgtccac cgccggcggc gacacgacgc cgagctcgat cggcccgagt
     1141 cgcaggcgat gcgtaccgtc gaccagcacc gcgacgggtc gctcgggcgt gccggtgccg
     1201 acgtcgcggc aggcgtcgtc ggccaggcgt gcctgtccgt gcgccgccgc gcaggccgcc
     1261 atgaggaggg tgaacagggc ccggaggggc aggcgcgcag ggtgcggcaa gtgcgatcct
     1321 cgtcagcgcg cggcccgtgc cgcgcgtcca gcggcgcagt ctaccgcctg gcgcggctca
     1381 ggccgcgacg cgctccacgc ggcacgagta gcacccgcgc ctggcgttgt gcaggtgcag
     1441 gaagtcgacg gccggatcct cgaacaggcg cgcgatggtc gcggcctgtg ccgcctcgcc
     1501 ttcgcagacg tcggccgcga gcatcatgcc gccggcgtcg taggcgcgcg ccgagaccag
     1561 gcgccgcgtc acatagggcg gcagcacgcc gggtgcgagg ctcgcccggc gcgcgccgcg
     1621 gcgcacgaag atcgggccgc cggcgcggta cggcgagtcg agcgccaggt gctcgtgcga
     1681 gagcagcagc agctcgtcgc cgacggcggc gtcctccagc gagatgcggc atgggaagcc
     1741 gaagggctcg tccgcgacgc agcgccggat cgcgcgggcg cgcagcgcgt cgtcgtcgag
     1801 gtcgaacagg gcctggaacg gatcggacgg gaggccgacg aggcggaagg agtgcatggc
     1861 ggcgacggca agcgggtgac gaccgatgca gtgtcccggc gcgccgcagc agccgcccgc
     1921 cgcattcgga catggaaatc gatgcccccg ggaacacggc cccgccgcgt cagcgcgctg
     1981 ccgactcctc ggccaccggc tgcgccggcg catgcggccg cacgcgcgcc gccggcacga
     2041 cgatgcggaa cgtcgacccg cgcccgagct cgctctggat gtcgagctcg ccgccgtggc
     2101 gctgcaccac gtgcttgacg atcgacaatc ccagcccggt gccgccggtc tcgcgcgagc
     2161 ggctgccgtc gacgcggtag aagcgctcgg tcagccgccc gatgtgctcg cgcgcgatgc
     2221 cggggcccgt gtccttgacc tcgatctcgg cgctgccgtc gctgcggcgg gaccaggtca
     2281 ccgcgatcga tccgccggcc ggcgtgtagc gcaccgcgtt gctcacgagg ttggcgatgg
     2341 cgctcgtcca ctcgctgtcg ttgccggcga tctcgtcgtc ctgcgcgatc gtgaagcgga
     2401 agccgtgcct gcccgccgag aggccgcgcg cctcgctctc cacctgcatc agcacccgcg
     2461 agacgggcgt ccagcggtcg catgccggcc gcgggctgcc ctcgagcttg gccagcgtca
     2521 ggagatcctc gaccagcgtg gtcatgcgcg aggtctgctg cgtcatcagg cccagcaccc
     2581 gctcgcgctc gacgtcggtc agcggcagcg tggagatcgt ctcgacgaag cccgccagca
     2641 cggtcagcgg cgtgcggatc tcgtgcgaga cgttggccac gaagtcgcgc cgcatcgcct
     2701 cggtgcgctc gcgctcggtg atgtcctgcg agagcacgag ccgcatgccc tcgccgtagc
     2761 ggctgacgat cacgctgagc gtcgcctcgc cgccgggcgc cgagaagacg atcgggtcgt
     2821 cgaactcgcc cgactgcagg taggccacga agctcggcga gcgcacgagg ttggtcacgc
     2881 gctgcaggcg gtcgcgcgcc gggtcgaggc cgaagtgcgt cgccgccatc gggttcagcc
     2941 actcgatctg gtccgccgag tcgagcagca gcacgccgtt gggcgacgcc tcgatcgcgg
     3001 acatgaactc ggcgaagcgc tcctgctcgc cgcgcagcgc ctggtcgcgc tggcgcagcg
     3061 cgcgctcgat gcggtaggcc gcctcgcccc acaggccggg cgcacgcggc gcctggcgct
     3121 cgtggtcgct gcgcagccag cgcatcaggc gatgcgcgcg cagctcgtcg acgagcgagc
     3181 gcgcgcccac cgcaagcgcc gcgcccacgg cggtgccgac gacgctcgcg tcgagcgcgt
     3241 gcccgaccag cgcgccgatc gcggcgccca gcagcgccag gacgacgtaa agcagcgcgc
     3301 gaccgagcga gttccccatc ggcgacgact caggccgggt tggcggcttg cggattggcc
     3361 gccggcagca cctgctgcgt gagccggtag ccggcgccgc gcaccgtctc gatcagcgtc
     3421 gagcattgca cgggcgcgag cgcctcgcgc aagcgcttga cgtgtacgtc gaccgtgcgc
     3481 tcttcgatga agacgtggtc gccccagacc cggtcgagca gctgggtgcg gctgtgcacg
     3541 cgctccgtgt gcgtcatgaa gaagtggagc aggcggaact cggtcgggcc gatcttgacc
     3601 tcgcggtcgc cgcgcgtgac ccggcgcgtg gccggatcga ggcgcaggcc cgagacctcg
     3661 accgccgagt ccagcgcctc gggcgccttg cggcgcagga cggcgcggat gcgggccagc
     3721 agttccttgg tcgagaacgg cttggtgagg tagtcgtccg cgccggcgtc caggcccgag
     3781 agcttgtcgg cttcgtccga gcgcgcggtc agcatgatga tgggcaggtc gcgcgtgcgc
     3841 gccaccgagc gccattgctt ggccagcgcg aggccggtct gccccggcag catccagtcc
     3901 agcacgacga gctcgggcag cacccggtcg acctcgcctt gcgcggcgtc ggcggtctcg
     3961 gcgatcgtca cctcgaagcc ggaatggcgg aggttgatcg cgatgagttc ggcgatcgcg
     4021 gattcgtctt cgacgacgag gatgcggctc atgctttggc tctcttcctg atgttctcga
     4081 acgtgcgcat ccccggaccg cggcgcgcgc agccctcccg ggcacggcac gcgcgccccg
     4141 ggacgacgcg gacggccacg gccgcccgcg gggcggttca gcggacggcg tcctcgaccg
     4201 cggccacggt gtggtggcgg acgtcggtgc ccttgacgat gtagatgatc tgctcggaca
     4261 ggttcttcgc atggtcgccg acgcgctcga tcgccttggc gacgaagacg aggtcgatcg
     4321 acgacgagat cgtgcgcggg tcttccatca tgtaggtgat gagcttgcgc agcaggccgt
     4381 cgaactcctt gtcgatctcg tcgtcctgct tgaggacctc gagcgcgcgg cccacgtcca
     4441 ggcgcgcgaa cgcgtcgagc accttgcgca gctgcgcggt cgcgaggttg gcgccgaacg
     4501 cgatgtcggc caccggcagg cgcaggcggc tcgagacgcc ggagttcatc aggcgctgca
     4561 ccgtgcgggc gatgcgcgtg gcctcgtcgc cgatgcgctc gaggttgccg atcgtcttgg
     4621 agatcgcgat cagcaggcgc aggtcgcgcg ccgtcggctg gcggcgggcg atgatggccg
     4681 acaggtcgcg gtcgatctcg acttccatcg tgttgacgcg ctgctcgcga tcgagcacga
     4741 ggctggccgt ctcgccgctg aagttggtca gcgcgtagac cgcttgcgcc acctgcgatt
     4801 cgaccagccc gcccatctcg agaacgcggt tggagatctc gctcagctcg gcgtcgaatt
     4861 gggtcgaaag gtgcttgtcc gtcatgtcgt gtccctgcaa atgcgctcgc gcgcgccgta
     4921 cggtaccggg tatgtccgaa gcgaggctga acctcagccg aagcggcccg tgatgtagtc
     4981 ctcggtgtcc ttcttcttcg gcttcatgaa caggtcggtg gtcttgccga actccaccag
     5041 gtcgccgagg tacatgtagg ccgtgtagtc cgagcagcgc gcggcctgct gcatgttgtg
     5101 ggtcacgatg accaccgtgt agtccgactt gagctcgtgg atcagctcct cgatcttgcc
     5161 ggtggagatc ggatccagcg cggagcacgg ctcgtccagg agcagcacct ccggcttgat
     5221 cgcgatgccg cgcgcgatgc acaggcgctg ctgctggccg ccggacagcg cggagccgct
     5281 ctggtccagc ttgtctttca cgtcgttcca cagcgccgcc ttcttcagcg cccactcgac
     5341 gcgctcgtcc atctcgacgc gcggcagcgt ctcgaacagg cgcacgccga acgcgatgtt
     5401 gtcgtagatc gacatcggga acggcgtcgg cttctggaac accatgccga ccttcgcgcg
     5461 gatcaggctg acgtccagcc tcgactcgag gatgttctcg ccgtcgagga cgatctcgcc
     5521 ggtggcgcgc tgctcgggat acagcgcgaa catgcggttg aaggtgcgca gcagcgtcga
     5581 cttgccgcaa cccgacggcc cgatgaaggc cgtgaccttg ccctcgggga tgtcgaggtt
     5641 gatgttcttg agcgcgtgga acgcgccgta gtagaagttg aggttgcgga cggcgagctt
     5701 cgccttctcg ccgacgggga gttcgacctt ggcatccatg gcgtggtgtc ctcgggttca
     5761 gttcttgttg cggaagaaga cgcgcgcggc gatgttcagc gccagcacgc ccagcgtgat
     5821 caggaacacg cccgcccacg cgatccgctg caggttgggg tacggctgca tggcgaagtt
     5881 gaagatcacc atcggcacgc tcgacaaggg cttggccagg ctcgtgctga agaactggtt
     5941 cgacagcgcg gtgaacagca gcggcgcggt ctcgccgacg atgcgcgcga tggccagcag
     6001 gatgccggtg atgacgcccg agcgcgccgc cgtcagcgtg accgacgaga tcaccttcca
     6061 cttcggcgtg cccagcgcgt aggccgcctc gcgcagcgca tgcggcacca ggttgagcat
     6121 gttctcggtc gtgcggatga ccaccgggat cacgatcagc gccagcgcca ccgagccggc
     6181 ccaccccgag aaggcccggg tcggcaccac gatcagctcg tagacgaaca ggccgatcac
     6241 gatggacggc gccgacagca ggatgtcgtt gatgaagcgc gtggtcgacg cgagccagct
     6301 cttctggccg tactcggcga ggtagacgcc cgagaagatg ccgatcggcg tgccgatgaa
     6361 ggtcgcgacc agcaccatca gcgccgagcc gtacagcgcg ttggcgaggc cgccgccgtc
     6421 ggacatggcc gcgggggtgg actcggtgaa cagcgccagg ttgaggccgc cgccgccgag
     6481 gcggacggtc tcgatcagga tccagacgag ccagaacacg ccgaaggcca tcgcgccgaa
     6541 cgcgagcgac agcgcgatca cgttgacacg cttgcggcgg ttgtgcatcg ccatccgggc
     6601 gggcggcagg gcggaaatcg cggtcatcgc gtggactccg tgttgcgttg ggccggcatc
     6661 acgacttcgc tccctcgccg cggcgcaggc gcatcagcag caccttggcg agcgcgagga
     6721 cgatgaaggt gatgacgaac aggatcagcg cgaggtacag cagcgcggcg cggtgcaggc
     6781 cctcgcccgc ctcggcgaac tcgttggcga ggacggaggt gatggtgttg gcggcctgga
     6841 acagggagat ctggctcagc tggctctggt tgccgatgac gaaggtcacg gccatcgtct
     6901 cccccagcgc acggccgagg cccagcatga tgccgccgat gacgccggtc ttggtgaacg
     6961 gcagcacgac cttgtagacc acttcccagg tcgtcgcccc gagcccgtac gccgactcct
     7021 tgagcatggg cggcgtgacc tcgaagacgt cgcgcatcac cgaggcgatg aacgggatga
     7081 tcatgatggc caggatgatc ccggcgcaca ggatgccgat gccaaccggc ggaccggaga
     7141 acagcgtgcc gagcacgggc acggtgccga acgcgttctg cagcgggccc tggacatagt
     7201 tggcgaggat ggggccgaac acggtcaggc cccacatgcc gtagacgatg gagggcacgc
     7261 ccgccagcag ctcgatcgcg atgcccagcg ggcgcttgag ccaggccggc gacatctcgg
     7321 tgaggaacag cgcgatgccg aagctcaccg gcacggcgat cagcagcgcg atgaaggagg
     7381 ttgccagcgt gccgtagatc atcgccaggc cgccgtagtg gtcctgcacg ggatcccaca
     7441 cgctggtcca gaggaaggac agcccgtagg cgcgcagcgc gggcatcgcg ccgacgatca
     7501 gcgagccgat gatgcccgcc agcatggcca gcgtgagcca ggcggcgccg tgcgcgagga
     7561 acgagaacag ggcgtcggcc cagggcgagt tctggcgccg ctcgggcgga cggccgaccg
     7621 gctgggtccc gtccttcgct ttgcgcaggt tcgattcggc gggcaatgtg gcagccacga
     7681 ggggcctccg gaagatggat gaatgccgcc ggccgacagt cggcgcggcg gcaacgatca
     7741 cgcgaggcgc gcgattactt gaaggcgacg gtcttgccgc tggagtcctt cacttcagcc
     7801 cactgcttgc ggatcaggct cttgaccgag tccggcagcg agacgtagtc gagttccgcg
     7861 gcggccttgt cgccgttggt gaacgcccag tcgaagaact tcatgacggc ggcgccctgg
     7921 gccggcttct cctgcgtctt gtgcagcagg acgaaggtcg cgccggtgat gggccaggag
     7981 tccttgcccg gctcgtcggt cagcacctgg tagaaggtct tggaccagtc ggcgcccgcg
     8041 gcggcggcct tgaaggtcgc gtcgtccggc gacacgaagg cgcccgcctg gttcttcatc
     8101 tggacgtagt ccatcttgtt ctgcttggcg taggcgtact cgacgtagcc gatcgagttc
     8161 ttcagctgca gcacgtactg cgccacgcct tcgttgccct tgccgccgac gccgttggcc
     8221 ggccagttca cggcggtgcc ttcaccgacc ttcgccttcc agtccgcgtt caccttggac
     8281 aggtagttcg tgaagatgaa gctggtaccc gagccgtcgg cgcggtgcac ggtcgcgatg
     8341 gcggcgtccg gcagcgtcac gcccgggttc agggcggtga tctccggcga gttccacttc
     8401 gtgaccttgc cggcgtagat gtcggcgagg acggtgcccg tcagcttcag ctggcccggc
     8461 ttcacgccgt ccaggtgcac gaccggcacg acgccgccca tgcaggtcgg gaactggacg
     8521 aggccggact gggccagctc gtcgtccttc agcggcatgt cggacgcgcc gaagtcgacc
     8581 gtcttggcct tgatctgctt gatgccggcg cccgagccga ccgactggta gttgacgtgg
     8641 ttgctcgaag ccttcgcgta ggcctcggcc cacttcgaat agatgggagc ggcgaacgag
     8701 gaacccgcgc cggtcacgtc ctcggcttga gcgacgaagg acgtcgcgcc gagggcggcg
     8761 gcgacggcga ccaggcgaat cggatggaag gtcatgatcc agggcctttc gagggcagtt
     8821 taatgagacc gcgccaatgt agaagcccct tgtgacaagt ggatgacagc tcgacgacgc
     8881 gcagcgctcg atccgctccc ggcgccccct cgcgggcggc cggtcgagcc gcgaacccgc
     8941 ctccgacctc gccggaaacg aatatgacag tcgtcacgac cgggtgtcat atccgtcacg
     9001 aaattgtcat tcgagctttg cggcctcggc aagacgctcc gcgcagcgcc gcgcgagcgc
     9061 cccgtcgcga gcctccacca tcacccgcaa caccggttcg gtgccggaag cacggatgag
     9121 cacccggccg tcgtggccga gctcgcgctc cacctcggcc ttgcccgcga ccagcgcggc
     9181 gttgtcctgc cagtcgcgcc gcgcggccag ccggacgttg atgagcacct gcggaaacag
     9241 ggtgacgccc tccagcagtt cggccagcga acgcccgcct cgccgtatcg cctggagcac
     9301 ctgcagcgcg ctgatgatcc cgtctcccgt cgtgtggcgg tcgagcacga gcaggtggcc
     9361 ggagttctca ccgcccagct gccaaccgcg gccgagcagt tcctccagca cgtaccggtc
     9421 acccaccttc gcccggacga gcgggacgcc gcggccgagc aaggcctgct cgaccgccat
     9481 gttggtcatc agggtgccga ccacgcccgg caccggctcg ccgcggccga ggcggtcggc
     9541 gacgacggcg tacagcagtt cgtcgccgtc gaagcagcgg ccgctggcat cgaccatctg
     9601 cagccggtcg gcatcgccgt cgagcgcgac tccgtagtcc gcgcggtgct cggccacggc
     9661 gcgttgcagc gcctggggcg ccgtcgcccc gacgcccgcg ttgatgttga agccgtccgg
     9721 ctcgcaaccg atcgtcacga cctcggcacc gagctcgtgg aagacgctgg gcgcgacctg
     9781 gtaggcggcg ccgtgagccg catcgacgac gatccggagg cccttgagcg acaggtcgga
     9841 ggcaaaagtg ctcttgcaga actcgaggta gcgccccgcc gcgtcattga tccggcgcgc
     9901 cttgccgagg gccgccgagt cgacccactg cggcgcttcc tcgagcgcgg cctcgacgtc
     9961 gagctcccac tggtcgggca gcttctcgcc cgacgcggag aagaacttga cgccgttgtc
    10021 gccgaacggg ttgtgcgagg cgctgatgac gacgcccagg tccagccgga gggcgcgcgt
    10081 caggtaggcg acgcccggcg tcggcagcgg gcccgtgagc agcacgtcga cgccggccga
    10141 ggcgaagccc gcctccagcg ccgactcgat catgtagccg gagacgcggg tgtccttgcc
    10201 gatcagcacc gtggcccgtg gagcgttgcg tcgcagcacg cgcccgaccg cgtggccgag
    10261 gcgcagcatg aaatcggccg tgatgggggc cgtgccgacc gtgccgcgga tgccgtcggt
    10321 gccgaagtgg gttctgctca tggttgtcgt cgtacgttgc agggaggtgc cgcacgcatg
    10381 cgcggcggcg tcgtgatcgg gaagcggccg gtcagccggc ggacagcgcc tgccagacgc
    10441 gcagggcgtc cacggtctgg gcgacgtcat gcacgcgcac gatggccgcg ccggcagcga
    10501 cggcggcgat ggccgcggcg acgctggcgg ccacccggtc gccgaccggc cggccggtga
    10561 gcgcgcccag cgtggacttg cgcgaccagc ccagcagcag cgggtgcccc agcgccagca
    10621 actcgcgctg tcgccgcccc aaggccagat tgtgcgcctg cgtcttgccg aagccgatcc
    10681 caggatcgag cgtgatccga tcggcggcga tcccggcggc cagcgcggca tcgcggcgcg
    10741 cagccaggaa gcgcgcgacc tcgccgacga cgtcgtcgtc cacgtagtgc ggcgcgacct
    10801 gcatcgtgcc cggttcgcct tgcatgtgca tcaggcagac gccggcgctc ggatggccgg
    10861 ccgcgacccc cagcgcgccc ggcgcctgca gcgcccgcac gtcgttgagg atgtcgacgc
    10921 ccaactcgag cgcggcctgc atcaccgccg ggtcggccgt gtcgaccgac accggcacac
    10981 ccagcgtcac ggcgtgccgc agcaccggaa gaacgcggcc gagctgctcg gcggtatcgg
    11041 gcaggcgtgc acccggacgc gtggattcgc cgccgatgtc gaggatgtcc accccttcct
    11101 cgagcagccg gtcgcagtgg gcgcaggcgg cgtcggcctg cgcgtagcgg ccgccgtccg
    11161 agaaggaatc gggcgtcacg ttgacgatgc ccatcacgcg cggccggtcc agcgcgatgc
    11221 ggaagcggcc ggcctgccag gcgcggatgg ccggcgggcc ggccggggga tcggcctgga
    11281 cgggaagcga ggacatgcgc tggacgggag aagcgaagcg acgcgagcaa gcggccgtcc
    11341 ggagcccgcg cccgccaagc ccgccgaaaa cgggcaacgg ggcgcgaggc cccgttcgtc
    11401 tggcgtgccg cgacgcggcg gtggcgaagc tccggccgac ctcaggccgt ggcgggcgcg
    11461 ccgtccggat tcagcggcgg cgggttgctg ccgccggagc ggttgatcga cggcgtccag
    11521 tccttcggcg gacgcggctc gcggccggcc atgatgtcgt ccagctgctc gcggtagatc
    11581 gtctcccact ccagcagcgc cttggcgatc gcctcgacct tgtcgcgctc cgtctcgatg
    11641 agggtgcgcg cgaggttgta ctggccgtcc aggatgcggc ggatctcggc atccaccttc
    11701 tgcatcgtcg cctcgctcat gttggtctgg cgggtgaccg agcggccgag gaagacctcg
    11761 ccctcgttct ccgcgtagac catcgggccg agcgattccg acatgccgta tttcatgacc
    11821 atgtcgcggg cgagctgggt ggcgcgctgg aagtcgttcg acgcgccgac cgtctgctgg
    11881 ttcatgaaca cttcctcggc gatccgaccg gcgtacagcg cggcgatgtt cgccagcatg
    11941 tactccttgt cgggggcgtg gttgtccttc tccggcaggt agaacgcgac gcccagggcg
    12001 cggccgcgcg ggatgatcgt caccttgtgc acggggtcgt gcttgggcag cttgagcatc
    12061 acgaaggcgt ggccggcctc gtggtatgca gtgttcttca gctcttccgg atcgatcacc
    12121 gcgctgcggc gctcggcgcc catgaagatc ttgtccttgg cgcgctcgaa gtcgatcatc
    12181 tcgaccgtgc gggcgctgcg gcgggcggcc atcagcgccg cctcgttgac caggttggcg
    12241 aggtcggcgc cggacatgcc cggcgtcccg cgcgcgatca cgttggcgtt cacgtccgtg
    12301 ccgatcggca ccttgcgcat gtggacgttg aggatctgct cgcggccgcg gatgtccggc
    12361 agcgtgacgt agacctggcg gtcgaagcgg cccgggcgca gcagcgccgg atcgaggatg
    12421 tccggacggt tggtcgcggc catcacgatg acgcccaggt tggtctcgaa gccgtccatc
    12481 tcgacgagca tctggttgag cgtctgctcg cgctcgtcgt tgccgccgcc caggcctgcg
    12541 ccgcggtggc ggccgacggc gtcgagctcg tcgacgaaga tgatgcaggg cgcgctcttc
    12601 ttggcctgct cgaacatgtc gcggacgcgg gccgcgccga cgccgacgaa catctcgacg
    12661 aagtcggagc cggagatcga gaagaacggc accttcgcct cgccggcgat ggccttggcg
    12721 agcagcgtct tgccggtgcc cggggggccg accatcagca cgccgcgcgg gatgcgaccg
    12781 ccgagcttct ggaacttctg cggatcgcgc aggaagtcga ccagctcctt gacctcttcc
    12841 ttggcctcgt cgcagccggc gacatcggcg aaggtgatgg agttgttcgc ctcgtcgagc
    12901 atgcgcgcct tgctcttgcc gaagctgaag gcgccgccct tgccgccgcc ctgcatctgg
    12961 cgcatgaagt acacccagac gccgatcagc agcagcatcg gcgcccacga cagcagcagg
    13021 ctggtgagca gcgagggctc gtcgcgcggc gtgatctcga acttcacgcc gttggccagc
    13081 aggtcgccga ccagcccgcg gtcgagcagg ctgctgccga ccacgtgctt cttgctgccg
    13141 ccgtcgctcg gggtcgcgac gatgtcgatc gtggtgccgc tctcggtgat caccgcgctc
    13201 gagatgcgct tgttatggac gtcgtccagg aactccgagt aggactcctt gatcccttgg
    13261 ctcgagccat ggtcgaacga tttgaagacc gtgaacagca ccagcgcgat gaccagccag
    13321 atcgcgatct tcgaaaacca ttgattgttc accgcggctc cttcttacaa aaatgccact
    13381 tcccacggca catggggccg attctaggcc agtcaatacc gtcccaaccc gattgctggg
    13441 gcgggatagg cggccaatcg cccgatttcc cgggtgaatc gtcgggcacg gggcagtttc
    13501 ggctcccgcc gcgcgtcagg cgggctttgc cggcgcgctc ttcaggccga aaccgacgat
    13561 gaaggtctcc gcggaccggt cccgcgacgc cttgggcttg atcgccttga cgactcggaa
    13621 gttcgccttg tagatctcca tcaactgact gtagccgctt ccgtgaaaca ccttggtcac
    13681 gagggctccc tcgggtttga ggtggtggat ggcgaagtcg atcgccagct cgaccaggtg
    13741 gctgacgcgc gcggcgtcgg tgtcggcgac gcccgagagg ttgggcgcca tgtccgagac
    13801 gacgacgtcc accggctggc cgccgaccag cgcctgcaac tgcgcgagga cggcatcctc
    13861 gcggaagtcg ccctgcagga agtccacgcc ctcgatcggc tccatcggca ggatgtccag
    13921 ggcgacgatg cgcccgttga gcgcgccgac cgccgccccg cccgccccgg ccgactttgg
    13981 cgcgaagcgg cgccgcaggt actggctcca cgcgccgggc gcggagccga ggtcgaccac
    14041 cagctggccg ggccggaaca gcttgaactg ctcgtcgatc tcctgcagct tgtaggccgc
    14101 gcgggcgcgg tagccgtcct tctgggcggc cttcacgtag gggtcgtgga cgtggtcgtt
    14161 gagccacgcc ttgttgacct tcttgctttt cgtgttcgtc ttcatggatg ttgggataat
    14221 aggcgatgcc cctgatcgaa ctgactcccg ccgaacgcaa ggaaaagcgc ggcgaagccc
    14281 atcacctgga tccggtcgtc atgatcggcg ccgatggcct gaccgccccc gtcctcaagg
    14341 aggccgaggc ggcgatcaag gcgcacggcc tcatcaaggt gcgcgtcttc tccgacgacc
    14401 gcgccgcccg cgaggccctg ctgctggaac tcgccgacaa gctcgacgcc gcgcccgtgc
    14461 agcacatcgg caagctgctg gtgctctggc ggccggtcga ggcgtcggcg cccgcgccgt
    14521 cggaggaccg cggcgccgcg ccgcgcgtcg tcaagatcct gaagttcggc aagcgtcccg
    14581 gccagcggcc cgagctgaag cgcgtcaggg tgctgggcaa ccagcgggtg accgcgggcg
    14641 ggcaggtcaa gcgcgcgaag aagcgcgtga cgagcatcaa gaagaaggtc gactgacccg
    14701 gcccggcggc cgcgccgcga tggccagtct gccaatcaat cccggcgcag cgccttccac
    14761 gccagcgcca gcaccatcag ccccttggcg gcgaagaacc cgaggctcac gaggtgcagc
    14821 tggccgaagc tgagcgccgt cgcccggccc gcgcgcgcct cctccatcaa cggctgcagg
    14881 ccgtagaagc cggccaccgt gcagaagatc gcaccgagcg cgagcatcat cccggaggtg
    14941 aactggctcg cgccggtcgc ctcgtgcgcg tcgacgccgg cgcgccgctc ggcgatcagc
    15001 accagcaccc cgagcgcgag gctcagcatg gcctcgcgct cgaagacgac ccgcgcgagc
    15061 tgccccgcgg cggcgcgatc cagcgccgcg aaggccgagg gcatcgccac gcccgcgatc
    15121 gccgccagca cgcccagcca gacgcccggg acgaagcggc gcagcagctc gggaagcggc
    15181 atcgacgcgg ccacgcggcg agggatcaga tgtacttgac cgcgacgatc tcgtagttct
    15241 tcacgccacc gggcgcctgg acggccgcgg tgtcgccggc ctccttgccg atcatcgcgc
    15301 gcgcgatcgg cgagcccacc gagatcatgc cttccttcag gtcggcttcg tcctcgccga
    15361 cgatctggta ggtgacctcc tggcccgatt cctcgtcctc gaggacgacc gtcgcgccga
    15421 agaccacgcg cccgccgccg tcgacctcgg aaggatcgat gacctgcgcc gcggccagct
    15481 tgccctcgat ctcgaggatg cggccctcga tgaagccctg cttgtccttg gccgcctcgt
    15541 actcggcgtt ctcggacagg tcgccctgcg cgcgcgcctc cgcgatcgcc tggatgacgg
    15601 cgtggcgctc cacggtcttg aggcggtgca gctcttcctt cagtttctcg gcgccgcggc
    15661 gggtcagggg aatcgtggcc atgtcgttga ctcgggtgaa aaagcaaacc gccgccccgg
    15721 cagtggatgc tccactggcg ggcggcggcc ctggttgtca ggcgggagtg tagtccaagt
    15781 ttcgacgggg gcaagccgga cgcccacccc cgccgggacg ctcactggag ctcggcgtgc
    15841 agctcctgca gcgagaccac ggtgatgtcg tcgccgtgct tgagcgcctc ggtcgcggcc
    15901 tcgcagccgg ccatcgtcgt gtagtacgtg acgcggttgg cgatggcggc cgtgcggatg
    15961 tagcgcgaat cggcgatcgc cgtgcgcgtc tcgtcgaccg tcgtgaacac cagctgcacc
    16021 tcgcccgcct tgatcatgtc ggcgatgtgc gggcggccgt ccttgacctt gttgaccagg
    16081 cgcaccggaa tgccggcctc ggcgatggcc gcggcggtgc ccttggtggc cgagatcgtg
    16141 aagcccaggt tgaccaggtc gctcgccacc ttgaccgtgc gcgccttgtc ggcgttcttc
    16201 acggtcagga cgacgttgcc cttggtcggc aggcgcgagc cggcgccgag ctggctcttg
    16261 agcatcgcct ccgcgaaggt gcggccgacg cccatcacct cgccggtgga gcgcatctcg
    16321 ggccccagca ccgggtcgac gccggggaac ttgttgaacg ggaagaccgc ttccttcacg
    16381 gtgaagtacg gcggcaccgt ctcgaccggc ggcttgccgc ccaggccctt ctggtcggcg
    16441 agcttcgtgc ccgccatgca gcgggcggcg atcttggcca gcgactggcc ggtcgccttc
    16501 gagacgtagg gcaccgtgcg cgaggcgcgc gggttgacct ccagcacgta gaccacggcc
    16561 tcgtcgccct cgccctggat cgcgaactgc acgttcatca ggccgacgac cttcagcgcg
    16621 cgggccatca gcgccgtctg gcggcgcagc tcgtcctgca gcgccggcga cagcgagtac
    16681 ggcggcagcg agcacgccga gtcgcccgag tgcacgccgg cctgctcgat gtgctccatg
    16741 atgccgccga tcatgacgtc ggcgccgtcg ctgatgcagt cgacgtcgac ctcgatcgcg
    16801 tcgtcgagga agcgatcgag cagcacgggc gacttctcgc tgaccttgac ggcctcgcgc
    16861 atgtagcgct cgaggtcctt gtcgccgtgc acgatctcca tcgcgcggcc gcccagcacg
    16921 tagctcgggc gcaccaccag cggataaccg atctcctgcg ccagctgcag cgcggcctct
    16981 tccgtgcgcg cggtgcggtt gggcggctgc ttcaggccga gcgtgtgcag cagctgctgg
    17041 aagcgctcac ggtcttcggc gatgtcgatc gagtcgggcg tcgtgccgat gatgggcacg
    17101 ccgttggcct cgaggtccag cgccagcttc agcggcgtct ggccgccgta ctgcacgatc
    17161 acgccgtgcg gcttctcgat cgcgacgatc tccagcacgt cctcgagcgt caccggctcg
    17221 aagtacaggc ggtcggaggt gtcgtagtcg gtggacacgg tctccgggtt gcagttgacc
    17281 atgatggtct catagccgtc ctcgcgcatc gcgagcgcag cgtgcacgca gcagtagtcg
    17341 aactcgatgc cctggccgat ccggttcggg ccgccgccca gcaccatgat cttcttgcgg
    17401 ttcgagggcg cggcctcgca ctcgccgtcc tcggtctcgt aggccgagta catgtaggcg
    17461 gtctgggtcg cgaactccgc ggcgcaggtg tccacgcgct tgtagaccgg gcgcacgccg
    17521 agcgcctggc gggcgcggcg cacgtcgtgc tggttggact tcagcagcgt gcccaggcgc
    17581 ttgtcggaga agcccttctg cttgacccag cgcagctccg ccgcggtgag cgactcgagc
    17641 gtgcggccgc gcagctggcc ctcgaccttc acgaggtcct cgatctgcgc gaggaaccag
    17701 gggtcgatcg ccgtctgctc gtgcacctcg tccagcgtca tgccgatgcg gaaggcgtcg
    17761 ccgacgaaca ggatgcgctc cgggccggcg ttggcgatct gctcgacgat ctcctcgcgg
    17821 tcggcctcgc ggcaggcggt gcgctcggtc aggccgtcga tgccggtctc caggccgcgc
    17881 agcgccttct ggaagctttc ctggaacgtg cggcccatcg ccatcacctc gcccaccgac
    17941 ttcatctgcg tcgtcaggtg cgggtcggcc tcgcggaact tctcgaacgc aaagcgcggg
    18001 atcttggtga cgacgtagtc gatcgagggc tcgaaggacg ccggcgtcgc gccgcccgtg
    18061 atgtcgttgc gcagctcgtc gagcgtgtag cccaccgcga gcttggcggc gatcttggcg
    18121 atcgggaagc cggtcgcctt ggaggccagg gccgaggagc gcgacacgcg cgggttcatc
    18181 tcgatgacga ccatgcggcc gtcggccggg ttgatcgaga actgcacgtt ggagccgccg
    18241 gtgtccacgc cgatctcgcg caggatcgcc accgaggcgt tgcgcatcag ctggtattcc
    18301 ttgtcggtga gcgtctgcgc cggcgcgacc gtgatcgagt cgcccgtgtg gatgcccatc
    18361 gggtcgaggt tctcgatcga gcagatgatg atgcagttgt ccgccttgtc gcggacgacc
    18421 tccatctcgt actccttcca gccgatgagc gattcctcga tcagcagctc gttggtcggc
    18481 gagaggtcga tgccgcgctt gcagatctcc tcgaattcct ccgggttgta ggcgatgccg
    18541 ccgcccgtgc cgcccagcgt gaacgacgga cggatcacca tcgggaagcc cgtgccgccg
    18601 atctcggccg cgatcgactt ctggacggcc caggcctcct ccatcgagtg cgcgatgccg
    18661 gacttcgccg agcccaggcc gatcttggtc atcgcgtcct tgaacttcag gcgatcctcg
    18721 gccttctcga tcgcatgctc gttggcgccg atcatctcga cgccgtattt cgcgagcacg
    18781 ccgtggcggt gcaggtcgag cgcgcagttc agcgcggtct ggccgcccat cgtgggcagg
    18841 atcgcgtccg ggcgctcctt ggcgatgatc ttctcgacca cctgccaggt gatgggctcg
    18901 atgtacgtca cgtccgccgt gttcggatcg gtcatgatcg tcgccgggtt ggagttgacc
    18961 aggatgacct tgaagccttc ctcgcgcaac gccttgcagg cctgcgcgcc cgagtagtcg
    19021 aactcgcacg cctggccgat gatgatcggg ccggcgccga tgatgaggat ggactggatg
    19081 tctaggcgct taggcattcg gggacttctt ccgggactgc gcttcggcca tcagcgaggt
    19141 gaagcgatcg aacaggtagg cgatgtcgtg cgggccgggc gaggcttccg ggtggccctg
    19201 gaagcagaac gccggcttgt cggtgcgcgc gagaccctgc agcgtgccgt cgaacagcga
    19261 gacgtgcgtc ggcacgaggt tggcgggcag cgtggcgggg tcgacggcga agccgtggtt
    19321 ctggctcgtg atcgagacgc ggccggtcgc gttctccttg acagggtggt tcgcgccgtg
    19381 gtggccgaac ttcatcttga aggtcttcgc gccggcggcg agcgccatga tctggtggcc
    19441 caggcagatg ccgaaggtcg gcaggccgga ctcgacgatc gtgcgcgtgg ccgcgatcgc
    19501 gtagtcgcag ggctcgggat cgccagggcc gttcgagagg aacacgccgt ccggattaag
    19561 cgccagcacc tcggccgcgg gcgtcttcgc gggcacgacc gtgatgcggc agccgcgctc
    19621 ggccagcatg cgcaggatgt tgaacttgac gccgtagtcg taggcgacga cgtggaagcg
    19681 cggcgactgc agcgtgccgt agccgctgcc gagcttccac tcggtctgcg tccactcgta
    19741 gcgctcggtg cagctgacga cctgggcgag gtcgaggccg gccatgttct cgccggcgcg
    19801 tgctcgcgcg atcgcgtcgg cgcggtcggc gtcggtcacc gcgtgcccgg ccgggaacgc
    19861 gacgatgcag ccgttctggg cgccgttcgt gcgcagcagg cgcgtgagct tgcgcgtgtc
    19921 gatgccggcg atcgcgacgg tgccctcgcg cgcgaggtac tcgtcgagcg acagcgaggc
    19981 gcggaagttg gagcgtcgca gcggcagatc cttgatgatc agcccggcgg catggacctt
    20041 cgacgcctcg acgtcctccc cgctgacccc gtagctgccg atgtgcggat acgtgagcgt
    20101 cacgatctgc ttgcagtagc tcgggtcggt gaggatttcc tggtagccag tcagcgcggt
    20161 gttgaagacc acttcgccga ccgtatggcc gggcgcgccg atcgaggtgc cggagaagac
    20221 cgtgccgtct gcgagtgcaa ggattgcggg gggcaggaca ggcagcaaag ggaactccgg
    20281 agtagctgtt gcccagttcg cgaggccccg tgcgcgcgcg gccgccgggc gggccggcag
    20341 gcgttgcgtg gggagtccga ttgcggtaga cgtagccgga gttttggcgc aggggttcag
    20401 gctgcgcggc ggtgatctcg gaggtctggg ctgattgcgg gtaaacctgt aaattgtagc
    20461 ctgcaccggc gtgcttgtca cccccttgtc atcggcgagc gcgtcgccag atgggtggcc
    20521 tggggcgccg cggaacttac aatggtccgg cgccgtcgaa cgcgacgggg atcgccccga
    20581 acattcctgg agagtgaaca catcatggaa cgcaacgtgc aacgcctgca aaccctgaac
    20641 ggcggcaacg tctacgggtt cgaccagcac cgcgtgctgc gcaacacgta ctggctgctc
    20701 gcgctgtcga tgatcccgac cgtggccggc gcatggctcg gcctggccac cggcttcacc
    20761 cgcatgatgg ccccgggcct gtcgctggtg ctgttcatgg tcggcgccta cggcctgatg
    20821 gccctggtgc acgcctggcg caactcgtcc aagggcgtgg tggccatgct cggcttcacg
    20881 ttcttcatgg gcctgatgct gtcgcgcctg ctcggcttcg cgctcggctt cgccaatggc
    20941 agcgcgctga tcatgaccgc tttcggcggc acggccggcg tgttcttcgg catggccacg
    21001 ctggcgacgg tcgtcaagcg cgacctgtcg gcgctgtcgc gcttcctgtt cgtggggatc
    21061 atgatcgcga tcgtcgcgag cctggtgaac ttcttcgtgc agtccacggc cgcgttcctc
    21121 gcgatctcgg tcgccgtgac ggcgatcagc tcgctgttca tcctggtcga cgtcaagcgc
    21181 gtgatcgacg gcggcgagga caactacatc acggccgcgc tgtcgatcta catcgacctg
    21241 tacaacgtgt tccagaacct gctgatgctg ctgacgatct tcaccggcga tcgccgctga
    21301 tcggctggcc agcgaacgaa aggcgcccgc gggcgccttt tttcatggcc ggcggatcgc
    21361 gcggcgtcag cgcgcgcgca gccggcggcc cagcagagcg accgcggcga gcccggccac
    21421 gagcagcgat cgcggcgccg gctcgggcac ggcggccgcg ggctcgaagg tcgcgatgct
    21481 cgacgcgccg agctcggcga gcgcgttctc gtcgaacgtg gtggcggtcg agcgcaacag
    21541 caggaagtag ctctcgctgc cgggcccgaa cgccgacggg tcgaaatccc aggtcagcga
    21601 ggtcggcgtc gcccacgcgt ccgtcggcgc catggtgcca aggccgtcga tccggtagtc
    21661 ggcatcgaag gcggcgccgg tgccgaagtc ggcgagcgtc aggcccgcga tcgtcagcga
    21721 cgtgggcacg tagctcgggt aggaagcgaa gtcgacgtcg accttccagt agaagtcgag
    21781 cgtgcccgtg ccggtctcgc gcacgacgcg cgactgcagc tcgccggtcg cgccggggaa
    21841 gccgtactgc gggtcgtcgg ccgagaccca accggtgacg acgtcctcga ccaccgtgcc
    21901 cgcgagcgcc ggatcggcgg cctcggtggt gccgggctgg acggtcgggc cggggccggg
    21961 cgcgacgggg acggcgcggg catcgagcgc gcacagcagc gccaggagcg tcgcggatgc
    22021 cagcgcggcg cgacggaagg caggacgtgc gttcgcgcgc ggccgccgct cagcccggat
    22081 ccgggctccc ggacgggcct tcggcgagcc gcgccgcgga cgcgcggtcc acgaagatcc
    22141 agtcgttggc cgcgttgcgc aggaacaggc cgtcgagcgc gagcatcttc gcggccatct
    22201 cgagcgcgcg ctcccacggc atgccaccga agctgggacg caggtccgga tgccgccgtg
    22261 agcacacggc atcgacgccg cgccggcccg ccgcctcgaa ggtcgcgacc tgcgcgggcg
    22321 cgccaccctg cccgggcgtc gcgttgaaat agaccgtgcg gccgcgcatc gcccgcagca
    22381 ggcggtcgtg ggccgccgac gtcccgatgc gcgaggcatc gcggacgagg gtctcgagct
    22441 cggcgtcgtc catggcagcc gccacgtcag ttcgtgcccg aggcgcggcg gcgcagctcg
    22501 gtgcgcgaga ccggcacgtc ggacggcgca ccttcctcgg gcggcgcggc ctcgccgagg
    22561 cgggccagga tgtgcggctt gtccatcgcc acccagcgat cctcgaggtt gctcagcacc
    22621 aggccctgcg catccggcat gcgtgcgacc atctcgagcg cgcgctccca tggcatgccg
    22681 ccaaacttct cctgcctgag gcgcggatgc tcgcgcgacg agaacagcgg cagcgcccgc
    22741 aggcccggcc ccaccgcgac cagcggcgtg cgcatctcct cgcccggact caggttgaag
    22801 aacacctctt tgccgcgaag gcgctccagc agacgcgcac gggctgccgg catgttggcc
    22861 tgcgccgcgt cggcgatagc gcgttcgatg tcgggatcgg tcacggcgtg tcagcggcgt
    22921 cttcgcccgc ctcgtcctgg gcgtcgccgc ccaccgcctc gtcgaagacc ggcgccgcgg
    22981 cgcgcggctg ccagtcggga tcccagtcga acacggccat gctctcgacg tgcgccgtgt
    23041 gcgggaacat gttgaccgcc ccggccgcga cacagcgata gccggcctgg tgcacgagca
    23101 gcccggcgtc gcgcgcgagc gtcgcggggt tgcagctgac gtagacgatg cgccggggcg
    23161 ccgtccagcc cgcgcgcggg gcggccttga gttccgccag cgccttggcg agcgcgaacg
    23221 cgccctcgcg cggggggtcg accagccact tgtcgacggt gcccagcgcg accaggtcgt
    23281 cgggcacgag ctcgaacagg ttgcgggcat cgaagcgcgt gcgcgcgtcc aggccgttga
    23341 ccctggcgtt cgccagcgcg cgctcgacca gcgtctggct gccctcgatg cccagcacgg
    23401 acctcgcctg cgtggccagc ggcagcgtga agttgcccag gccgcagaac cagtcgatga
    23461 cgtcctcgtg cgactgaacg tccagcagcc gcagcgcacg gccgaccagc accgtgttga
    23521 tctggtggtt gacctgggtg aagtcggtcg gcctgaacgg catgcgcacg ccgaactcgg
    23581 gcagcaggta ggcgagctcg ggcccgtcct cgtcgatacg gtggatggta tccggcccct
    23641 tgggctgcaa ccaccactgc acggcgtgct ccgcgccgaa cgcgcgcagt tcgccgaggt
    23701 cgtgctccga cagcggatcg aggtggcgca gcaccagcgc cgtcacctgg tcgccgatcg
    23761 cgacctcgat ctgcggcagc cggtcgcgcg cctgcatgcg gccgacgagc tcgcgcagcg
    23821 gcagcagcat gtcggagatg cggcgcggca ggacgtggca ctcgcggatg tcggcgacgt
    23881 agctggactt gcgttcgtgg aagccgacca gcaccgtgcc cttcttggcg acgtgacgga
    23941 ccgacaggcg cgcgcgcagc cggtagcccc aggccgggcc ctcgatcggc cgcagcacga
    24001 tctcggggcg caccttgccg aggtgccaga ggttgtcctc gagcacgcgc tgcttcatcg
    24061 ccacctgcgc ggccggcccg aggtgctgca tcttgcagcc gccgcacgct ccggcatgca
    24121 ggccgaagtg cgggcagcgc ggcttcacgc gttgcgcgct ctcgcgccgc agcgcgacga
    24181 gctcggcctg ctcccactgc ttcttgcggc gcacgacatg cacgcgcgcc tcctcgcccg
    24241 gcagcgcgcc gtcgatgaag acgaccttgc cctcgctgtt gtgggcgatg ccctgcgcct
    24301 cgaggtcgat gcgcaggacc ttcagccatt cggaatcggt cacgccgggc gcgggttcgg
    24361 cgcggcggtg gttgcgtccg cggcgctcct tcttgccgcc ggcggtcggt tggtcggtac
    24421 tcatccccga attatcgacg cccggacgac gacggccccg acaccggtgg atgtcggggc
    24481 cgccgtggcc ggtcgcgcgc cgggatcagt gcgtcgcgag gcggaacacg ctcacgtcgc
    24541 tcacgaggcg ctgcacctgc gagcgcatcg agtcggccgc cgacgccacc tgctcgacca
    24601 gcgcggcgtt ctgctgcgtg acctggtcca tctcgctgac ggcgctgttg acgaggtcga
    24661 tgcccgcgct ctgctccacg gtggcggagg tgatctccga gacgatgccc gccacgtgct
    24721 gcacggactg cacgatctgc gcgatggtgc cggcggcgac gtcgaccagg tgcgtgccct
    24781 cgtccacctt ctcgaccgac tcggcgatca gcgacttgat ctccttggcg gccgtggcgc
    24841 tgcgctgggc cagcgtgcgc acctcggtgg cgacgaccgc gaagccgcgc ccctgctcgc
    24901 cggcgcgcgc ggcctcgacc gccgcgttca gcgcgaggat gttggtctgg aaagcgatgc
    24961 cgtcgatgac gctgatgatg tcggcgatct tcttcgacga cgaggcgatg tcgcccatcg
    25021 tgcgcacgac cttgctcacg gcctcgccgc cctgctgcgc cttgtcggcg gcctcggtgg
    25081 acagctggcg cgcgtgggtc gagttgctcg cgctcatctt cacggtgctg gagagctcgt
    25141 ccatcgagga agccgtctgc tggatgctcg cggcctggcg ctcggtgcgg ttcgagaggt
    25201 cggcgttgcc ggaggcgatc tcggtggcgg cgccggcaag gctgtcgctg ccgttgcgca
    25261 cgctggcgac gacggtcacg aggctctcgg tcatcgcctt gagcgccttc agcagctggc
    25321 cggtctcgtc gtccgagctc gagtcgacgc gcaccgtcag gtcgcggtcg gcgacgcggc
    25381 gggcgatctc gacggccttg gagatcggca ccgtgatcga gcgcgtcacg gccaggccga
    25441 tccacgacgc gaacaccagc gcgacgaccg agatgccgag cacgatgttg cgcgtggtct
    25501 cgtaggcccc ctcggcgccg gagacggcgg ccgcggtcag cttctcgttg agggcgacca
    25561 tctcgtcgac ggcggccatc cacttcgcct gggccgggtt ggccttgtcg ccgagcaggc
    25621 cgcgggcggg aaatttgtgg ttgtcgagcg ccatctgcac gacctggccc gagaggtccc
    25681 gcgcttcctt ctctgccgcg tcgatgtggc cgaggatggt cttcttgcgg gcgtcgccgt
    25741 cattgccgat cagcgtggcg agccgggcct tggccgtgtc gtaggtcttg atgtcggcct
    25801 tgagcagttc gttctcggcg gccatctcct cgggctcgga gaccatcagg atgttgcgca
    25861 cccgcacggc ctgctgcgtg gcggccaggc gcatctggtc ggcgagcgag gattccaccg
    25921 cgatcccccc cgtgaccgcg ttcatgtcgt tgcgcatgct gccggtgcgc acgagcgcga
    25981 tgatcgacac cgtggccagc agcgccagca cggtgccgaa ggcgagggcg agccggacgc
    26041 cgatcttgag gttggtcaga cgcatgtttc ttgttccgaa ggttgggcgt ggggatgggt
    26101 cggtggtcgg tgttgtcgtc tgacgcgccg tctccctcgt gcggcgttct ctgtcagggc
    26161 gtgtcgccac gggagccgga ccgggctcgc cggcccgcgg tccgcgtcgt cagttcagct
    26221 tccagacgaa ctcggtcttc acccattgct gcaccggctt ggcgtcggcg ggcgagggct
    26281 tgaacgagca ctccatcagc gcgcgctggg cggccgagtc gagcttgcgc gaatggctgg
    26341 acgactcgat ctcgctgtcg atggccttgc cgtcgacgcc gacgaggatg cgcaggccgg
    26401 tcgtgccctg ctcgccctta ctcagcgact cgcgcgggta ttccggcggc gcgcaactct
    26461 tggcgtcctg cacccacttg aagtcggccg ccagggcgcc ggcgctggca agggcagcaa
    26521 gcgcgccggc ggcggcggcg cgaatcagtc gggaacggac gtgcggcttc atgttgggct
    26581 cctgcggatc ttgaaaaagg acggttctcg accccgtccg cgtccgccgt gtaggggacc
    26641 ttggatgggg ccatggcccg atttcgtctg caatgcccga ttcttgagtg gcatcggtca
    26701 cgacttatcc ggaaacagcg gcggtttccg ggcccaatcc cccgcctgag cccctcaggc
    26761 gccgtgactc ctgcggcgga acccccgatt ccgccgaaga ttccgaataa acgtcggggg
    26821 atgccccagg ccgaaacgaa aaacgcgccc tcgggcgcgt cttcgtcggc ggcggcagcg
    26881 cttcagcgcg gcggcagcag cttggcggga tccaccggct tgccctggcg gcggatctcg
    26941 aaatggagct ggacgcggtc ggtgtcggtg gaacccatct cggcgatctt ctggccgcgc
    27001 ttgacgaact ggtcctcctt gaccagcagc gtctggttgt gcgcatacgc cgtgaggtag
    27061 ttcgcgttgt gcttgacgat cacgaggttg ccgtagccgc gcagcccgga gccggcatag
    27121 acgaccttgc cgtccgccgc cgcgtagacc gggtcgcccg ccttgccggc gatcgacagg
    27181 cccttgctgc gggcgtcgtc gaacggcgcg acgacggcgc cgttcgcggg ccaggcccag
    27241 ttcggctcgt cgtcgttctc gtgggcggcc ggcggcggcg tcgcgacgtt ggtcagcggc
    27301 gccggggtcg tggcgctcgc gttcgaggcc gccgggggca gcgccacggc ggagccggcg
    27361 acggcgacgc cactcgcctt cggctcgggc ttgtcggtct tggccggcag cggcttgaag
    27421 gtcgtcgtgg tcgtcgtcgt ggtggtgttg gtggtctcgc cggggggcgg caccacgcga
    27481 agcacctggc cgacctcgat cttgtccggg tcggtgatgt tgttccagcg cgcgagatcc
    27541 ttccagccct ggccggtgtt caggccgatc cggctcagga tgtcgcccgg ctggacgatg
    27601 taggtgcccg gcttggcctc gaccggcggc gcgacgggcg cgctcgcctt ggcgggctcg
    27661 gccgcgggcg cgaggtgccc ggcggtggac cgggagtggt cctcgaccgg cgcctggttc
    27721 gtggaggcgc acccgatcaa ggcccatgcg gacgccgcgc taaccgtcgc cacgagcgta
    27781 cgtcgacccc aatgcgatgc catagtttcc ctgcagtgat tcaaccaatc cccgatttta
    27841 ggggaacaaa cgtgacggac tccccgacct ggcggacgaa gccgcccggc gtccggtcga
    27901 cgatcaccag ggcctgaccc gcgaggcccg gcatggccgt cggcgccacc agccgtccgc
    27961 cgggcgcgag ctgctgcagc cacgcgtccg ggatcgcctc gccgcccgcc gcggcgacga
    28021 tgctgtcgta gggcgcgttg ggcgggtggc cgtggcggcc gtcgccgtgc acgagccgca
    28081 c
//
LOCUS       DASVLN010000575         3068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_30646, whole genome shotgun sequence.
ACCESSION   DASVLN010000575 DASVLN010000000
VERSION     DASVLN010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30646"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(388..1035)
                     /locus_tag="VF453_13870"
     CDS             complement(388..1035)
                     /locus_tag="VF453_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7688795.1"
                     /translation="MSDALPPDRIRLLLVDDHPLVRDGLRARLEAVPGFEVAGEAGDA
                     AEAQAQVDALRPGLVLMDVGMKDVNGIDLTAQLLERDPGLQVLMLSMYDNPEYVQRAM
                     QAGARGYVLKDSPASEIVAAIEAIAAGGTFLSPAVSRRLFRNQAPRPLLSSRESQILS
                     ALARGLSSKLIAREMDVSVRTVEAHRQSIKRKLALEGQAELIKYAVEHARVHGGA"
     gene            complement(1090..1719)
                     /locus_tag="VF453_13875"
     CDS             complement(1090..1719)
                     /locus_tag="VF453_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7688796.1"
                     /translation="MLKVLGRAPSINVRKVLWTCAEIGLQVEHEADWGTPARPASAPG
                     FLALNPNAQVPVIVDDGFVLWESNTICRYLAGRHGRDDLLPADPRARARVEQWMDWSA
                     TDLNAAWRPAYFALVRGDEAVRADVRAVEASAARWNALMGLLDAQLAHGGPYIAGATF
                     TLADVVLALATNRWRCTPIAHAKLPAVDAWFDRLRARPAFLRFCDNGQP"
     gene            complement(1738..2568)
                     /locus_tag="VF453_13880"
     CDS             complement(1738..2568)
                     /locus_tag="VF453_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX7688797.1"
                     /translation="MDIQRISAFSDGDAGGNPAGVVLCERTLPPVADMQRVAAEVGYS
                     ETAFAAPEGDAWQVRYFSPAAEVPFCGHATIALGAALARAHGDGTYALKLARADITVE
                     GRREGGRHAAALQSPATRSAPAEAALVDEVLALFGYARSQLDARIPPARIHGGGDHLV
                     LALDSRAALAAMRYDFEAGRALMLREGWITILLAWAESPTRFHTRNPFAYGGVYEDPA
                     TGASTAAFAGYLRDLGWPHGGRIDVVQGEDMGARSLLHADIGPVPGGSIRVSGTARDL
                     "
     gene            complement(2616..>3068)
                     /locus_tag="VF453_13885"
     CDS             complement(2616..>3068)
                     /locus_tag="VF453_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688798.1"
                     /translation="RPSAPAPASTSTDEAFRGVRFGASRAEVRRAYPATVCGAGECRG
                     TTQVRGQPASFLVFAEADGTWAARLKLGDQGGRNFRALADELGQRFSRYAATIEQDGN
                     LLRWHDRARPRQVVMRRCVPGTRCPDGVAPDAVEIDFFEEGPPRAHAW"
BASE COUNT          404 a         1125 c         1160 g          379 t
ORIGIN      
        1 gcccggcctg cgcgggctgc gcccacgccg gcatgctgcc cgccgcgacc agcagcgcga
       61 ggcccggcag gcggcaggat cgccagcggc gcaacggacg gattcgaagg acagccgcgg
      121 cggcgatggc ggcggcggcg aggcaacgga tcatgcttgt ctcctggacg gccggcgtcg
      181 acgtgcgccg cactctcgca cccagtctgg ccgccggcgc gcgccccggc atcgtgcgca
      241 acgcggggcc ggcccgagcg gcatcacgta ggtgcaaata ctgaatcggc cgcggcggtg
      301 aagtcgcgga agcacggccc cggcgggccg tggattcacc gatccgtgag cgcgtcgccg
      361 aaggatcggc ggcggggcgc gcggcgatca ggcgccgccg tgcacccgtg cgtgttccac
      421 ggcgtacttg atcagctcgg cctgcccctc gagcgccagc ttgcgcttga tgctctggcg
      481 atgggcctcc accgtgcgga cgctgacgtc catctcgcgc gcgatcagct tgctcgagag
      541 cccgcgcgcg agcgccgaca ggatctggct ctcgcgcgag gacagcagcg gccgcggcgc
      601 ctggttgcgg aacaggcgcc gactcaccgc ggggctgagg aaggtgccgc cggcggcgat
      661 ggcctcgatg gcggcgacga tctcgctggc gggcgagtcc ttgagcacgt agccgcgcgc
      721 gccggcctgc atggcgcgct gcacgtactc ggggttgtcg tacatgctga gcatcagcac
      781 ctgcagcccc gggtcgcgct cgagcagctg tgccgtgagg tcgatgccgt tgacgtcctt
      841 catgccgacg tccatcagca ccagccccgg ccgcagcgcg tcgacctggg cctgggcctc
      901 ggcggcgtcg cccgcctcgc ctgcgacctc gaagcccggc acggcctcca ggcgcgcacg
      961 caggccgtcg cgcaccagtg gatggtcgtc gaccagcagc aggcggatcc gatccggggg
     1021 cagcgcgtcg ctcatggcgg gattagaccg cagcgccggc gccgtgcgtg gcgcggcgcg
     1081 tgcggatcgt cacggttggc cgttgtcgca gaagcgcagg aacgccggcc gcgcgcgcag
     1141 gcgatcgaac caggcgtcca cggccggcag cttcgcatgc gcgatgggcg tgcagcgcca
     1201 gcggttggtc gccagggcca gcacgacgtc ggccagcgtg aaggtcgcgc ccgcgatgta
     1261 ggggccgccg tgcgccagct gcgcgtcgag caggcccatc agcgcgttcc agcgcgcggc
     1321 gctcgcctcg accgcgcgca cgtcggcgcg taccgcctcg tcgcctcgca cgagcgcgaa
     1381 gtaggccggg cgccaggccg cgttgaggtc ggtcgccgac cagtccatcc actgctcgac
     1441 gcgggcacgc gcgcgcgggt cggccggcag caggtcgtcg cggccgtggc ggcccgcgag
     1501 gtagcggcag atcgtgttcg actcccacag cacgaagccg tcgtcgacga tcaccggcac
     1561 ctgcgcgttc gggttcagcg cgaggaaccc gggagcgctc gccgggcgcg ccggcgtacc
     1621 ccagtcggcc tcgtgctcga cctgcaggcc gatctccgcg caggtccaca gcaccttgcg
     1681 cacgttgatg gagggggcac gccccagcac cttcagcatc gtcgcctccc tcgcggctca
     1741 gagatcccgg gcggtaccgc tgacgcggat cgagccgccc ggcaccggcc cgatgtcggc
     1801 atgcagcagc gagcgcgcgc ccatgtcctc gccctgcacg acgtcgatgc ggccgccgtg
     1861 gggccagccg aggtcgcgca ggtagcccgc gaaggccgcg gtcgaggcgc cggtggccgg
     1921 atcctcatac acgccgccgt acgcgaacgg gttgcgggtg tggaagcgcg tcggcgactc
     1981 ggcccacgcg agcaggatcg tgatccagcc ctcgcgcagc atcagcgcgc gcccggcctc
     2041 gaagtcgtag cgcatcgcgg cgagcgcggc gcgcgagtcg agcgccagca ccaggtggtc
     2101 gccgccgccg tggatgcgcg ccggcgggat gcgggcgtcc agctgcgagc gcgcgtagcc
     2161 gaacagggcg agcacctcgt cgaccagcgc cgcctcggcg ggcgcgctgc gcgtggcggg
     2221 cgactgcagc gccgcggcat ggcgaccgcc ctcgcgccgg ccttcgaccg tgatgtcggc
     2281 gcgtgcgagc ttcagcgcat aggtgccgtc gccatgcgcg cgcgcgagcg cggcgccgag
     2341 cgcgatggtg gcgtggccgc agaacggcac ttcggcggcg ggcgagaaat agcgcacctg
     2401 ccaggcgtcg ccctcgggcg ccgcgaacgc ggtctccgaa tagccgacct ccgcggcgac
     2461 gcgctgcatg tcggccaccg gcggcagcgt gcgctcgcac agcacgacgc cggccgggtt
     2521 gccgccggcg tcgccgtcgg agaaggccga gatccgttgg atgtccatgt cgctgatgcc
     2581 tcctgatgcg ggtcgccgcc accgcggcgg ggagctcacc acgcgtgcgc gcgcggcggg
     2641 ccttcctcga agaaatcgat ctcgaccgcg tcgggcgcga cgccgtcggg gcagcgcgtg
     2701 cccggcacgc agcgacgcat cacgacctgg cgcgggcgcg cacggtcatg ccagcgcagc
     2761 aggttgccgt cctgctcgat ggtcgcggca tagcgcgaga agcgctggcc cagttcgtcg
     2821 gcgagcgcgc gaaagttgcg gcccccctgg tcgccgagct tcaggcgggc ggcccaggtg
     2881 ccgtcggcct ccgcgaagac gaggaagctc gccggctgcc cacggacctg ggtcgtgccg
     2941 cggcattcgc cggccccgca gaccgtcgcc ggatacgccc ggcgcacctc ggcgcgcgac
     3001 gcgccgaagc gcacgccgcg gaaggcctcg tcggtcgatg tcgatgcggg tgccggcgcc
     3061 gacgggcg
//
LOCUS       DASVLN010000576         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_300800, whole genome shotgun sequence.
ACCESSION   DASVLN010000576 DASVLN010000000
VERSION     DASVLN010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300800"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1333)
                     /locus_tag="VF453_13890"
     CDS             complement(23..1333)
                     /locus_tag="VF453_13890"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7688799.1"
                     /translation="MTPHRIVIVGGGAGGLELATRLGDRLGKRGRASVTLVDRHRSHV
                     WKPKLHEIAAGSMDVDEHELDYLAQSHWHHFAYRVGEMTGLDRAAREVLVAPYVDDEG
                     RQVTEAARIPYDTLVIAVGSQSNDFGTPGVAEHALKLETAGDARRFNRRLVNAAIRAN
                     AQSAPIAPQQLHVAIIGAGATGVELSAELHRTTRELIAYGLDRIDADRDLQLHLIEAS
                     ARILPALPERFSASTHKLLDDLGVQVHVNARVAEVLPERVRLADGREIAAELVVWAAG
                     IKAADFLKDLAGLETNRLNQLVVRPTLQSTRDDDVFALGDCAACEWPGHGMNVPPRAQ
                     AAHQQASHMVRQIERRLAGRPLKAYVYRDFGSLVSLGEYSTVGNLMGGLIGGNFFIEG
                     YFARLMYRSLYKMHELALHGWTKVWLATLARLITRRTEPHVKLH"
     gene            complement(1472..>1676)
                     /locus_tag="VF453_13895"
     CDS             complement(1472..>1676)
                     /locus_tag="VF453_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688800.1"
                     /translation="DTTSPDAAPVTAAALPLVVAPASPHPFAIAVVGDEAAGDLFGAL
                     ALVDTLVLAVAAGLAAWPVFMAA"
BASE COUNT          222 a          576 c          639 g          239 t
ORIGIN      
        1 gccccggcgc cgggcggcgt gatcagtgca gcttgacgtg cggctccgtc cgccgcgtga
       61 tgaggcgcgc cagggtcgcg agccacacct tcgtccagcc gtgcagcgcg agctcgtgca
      121 tcttgtacag cgaacggtac atcaggcgcg cgaaatagcc ctcgatgaag aagttgccgc
      181 cgatcaggcc gcccatcagg ttgccgacgg tgctgtactc gcccagcgag acgagcgagc
      241 cgaagtcgcg gtagacatag gccttcagcg gccggccggc gaggcggcgc tcgatctggc
      301 gcaccatgtg cgaggcctgc tggtgcgccg cctgcgcccg cggcgggacg ttcatgccgt
      361 ggccgggcca ctcgcacgcc gcgcagtcgc cgagcgcgaa cacgtcgtcg tcgcgggtgg
      421 actgcagcgt cggacgcacc accagctggt tgagccggtt ggtctcgagc ccggcgaggt
      481 ccttgaggaa atcggccgcc ttgatgcccg cggcccagac caccagctcc gcggcgatct
      541 cgcggccgtc ggccaggcgc acgcgttcgg gcagcacctc ggcgacgcgc gcgttcacgt
      601 gcacctgcac gccgaggtcg tcgagcagct tgtgcgtcga cgccgagaag cgctccggca
      661 gcgccggcag gatgcgcgcc gaggcttcga tcaggtgcag ctgcaggtcg cggtcggcgt
      721 cgatgcggtc gaggccgtag gcgatcagtt cgcgcgtcgt gcgatgcagc tccgcggaca
      781 gctcgacgcc cgtcgcgccg gcgccgatga tggccacgtg cagttgctgc ggcgcgatcg
      841 gcgcggactg cgcgttggcg cggatcgcgg cgttgacgag gcggcggttg aagcgccgcg
      901 cgtcgcccgc ggtctccagc ttgagcgcgt gctccgcgac gcccggcgtg ccgaagtcgt
      961 tgctctggct gccgacggcg atcaccagcg tgtcgtaggg gatgcgcgcg gcctcggtga
     1021 cctggcggcc ctcgtcgtcg acgtaggggg cgaccagcac ctcgcgcgcg gcgcggtcga
     1081 gtccggtcat ctcgccgacg cggtaggcga agtggtgcca gtgcgactgc gcgaggtagt
     1141 cgagctcgtg ctcgtcgacg tccatgctgc cggcggcgat ctcgtgcagc ttgggcttcc
     1201 agacatggga gcggtggcgg tcgaccagcg tgacgctggc gcggccccgc ttgccgaggc
     1261 ggtcgccgag acgggtggcg agctccagcc cgccggcgcc gccgccgacg atcacgatgc
     1321 gatgaggggt catgcgcgcc ctgtccgggt tttccacgaa ggccgacagc gtaccgtgat
     1381 ccggcccggt ttccgagcgg ttacttcgcg atcggagaag gccgggttcg gcgcgcttgc
     1441 gaacgggcgc gcgcgggcgc ggcggtcgag ttcaggccgc catgaacacc ggccaggcgg
     1501 ccagcccggc cgccaccgcg agcaccagcg tgtcgaccag cgccagcgcg ccgaacaggt
     1561 ctcctgcggc ctcgtcgccg acgaccgcga tcgcgaacgg atgcgggctt gcgggcgcga
     1621 cgaccagcgg cagagccgcg gcggtcacgg gcgcggcgtc gggcgaggtc gtgtcg
//
LOCUS       DASVLN010000577         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_461641, whole genome shotgun sequence.
ACCESSION   DASVLN010000577 DASVLN010000000
VERSION     DASVLN010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461641"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF453_13900"
     CDS             <1..415
                     /locus_tag="VF453_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7688801.1"
                     /translation="DEFARGDFGPQGGAAAMRRLLSAPVRPTAVFVCNDLMAIGALHA
                     AHELGVDVPGALSVVGFDDIELAAYTWPPLTTIAQPKEAIGTGAAGLLLERLQHGRNE
                     ARRMILEGNLRVRASSAPPGTMPARLSRSSQEHRT"
     gene            412..1350
                     /gene="rbsK"
                     /locus_tag="VF453_13905"
     CDS             412..1350
                     /gene="rbsK"
                     /locus_tag="VF453_13905"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX7688802.1"
                     /translation="MSATIVVVGSLNMDLVMRMTRAPVGGETLPGHDFAMLAGGKGAN
                     QAVACARMGASVAMVGRVGIDANGDALKAGLAADGIDAREVKALASTHTGVASIWVED
                     DGQNRIVLASGANGLLDAAAVARAHPMIDAAEMLIVQLEVPMPAVQAAVERAWRAGVP
                     VLMNPAPAAPLPDAMWGMIAILVVNETEAAALSGLPVVDPATAGAAGRALRARGPDRV
                     ICTLGGQGCVVVDADGARHTPAMVVKVVDTTAAGDTFIGALAVALCEGQPLDVAVAFG
                     QAASALCVTRRGAQVSIPYRRQVALPAATSPTPIPS"
     gene            1360..1755
                     /gene="rbsD"
                     /locus_tag="VF453_13910"
     CDS             1360..1755
                     /gene="rbsD"
                     /locus_tag="VF453_13910"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEX7688803.1"
                     /translation="MKRTVLLHAELSEVIARLGHGDLLVIGDAGLPIPDGPRRIDLAV
                     SRDVPRFHDVLAAVLAEMKVESAVVADELAGHNAVVNMELRRQLHGMVLETVPHADFK
                     SMTRTARAVVRTGECSPYANVILRAGVVF"
     gene            complement(1784..3682)
                     /locus_tag="VF453_13915"
     CDS             complement(1784..3682)
                     /locus_tag="VF453_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HEX7688804.1"
                     /translation="MNRPFPRALQLGALAAALGAAFAAPAGAAALNPNDTSVQMFEWA
                     WNDVATECTQWLGPQGYGGVQISPPGASKNATGWWGVYQPVNYVNLTSRFGNETQLKS
                     MIDTCHAAGVRVYADIVVNQLADGSSASNGVATDGSTWDSSTLAYPYFSANDFHANCA
                     IQSSDYNSPSGTWDVQNCRLGGLPDLDTESSYVQGQVENYLNHLLALGVDGFRIDAAK
                     HQAASSLQAIMSAVKAAHPTTSLGEPIWVTQEIIPDGEVSRSSYFPIGTINEFQFTYL
                     MRDAWRNQNGSSPSTIPSAMGTWNNWGGTWGFVQPTNATVFLNNWDSERDGSSLDADN
                     YTGSVTNDTQGTHRYDLANIFMLAQGYGEAQLYSGFRFTNSNQDRPSASPYSNGVPQI
                     NVNWDFLHRWTDISNMVKFRSAAHGQAQGNWVTGNANQIAFSRGNVGFVALNNTGSSW
                     SRTFYTGLPAGTYCNVIHGLKNAAGTACTSDSVTVDASGNAAITVAADGGSTVPAVAI
                     YTGQMIAGSGGGGGGATTCAVTFTIANANTSYGQNLYVVGNQSALGNWTPASGFALAI
                     QGSGANVPWTGTVALPANAAIQYKYVKWNGSTAVWESNQSTASGNRELTSCAAGGAVA
                     RNDGGFMN"
BASE COUNT          539 a         1356 c         1412 g          654 t
ORIGIN      
        1 agacgagttc gcgcgcggcg acttcggccc gcagggcggc gccgcggcga tgcggcggct
       61 gctgtccgcc cccgtgcggc cgaccgcggt cttcgtctgc aacgacctga tggccatcgg
      121 cgcgctgcac gcggcgcacg agctaggcgt cgacgtgccg ggcgcgctgt cggtggtcgg
      181 cttcgacgac atcgagctgg ccgcctacac ctggccgccg ctcaccacaa tcgcgcagcc
      241 caaggaggcg atcggcaccg gcgcggccgg cctgctgctc gagcggctgc agcacgggcg
      301 caacgaggcc cgcagaatga tcctcgaagg aaacctgcgc gtgcgcgcat cgagcgcgcc
      361 gcccggtacg atgcctgcca ggctttcgcg cagcagccag gagcaccgca catgagcgca
      421 acgatcgtcg tcgtcggcag cctcaacatg gacctggtga tgcgcatgac gcgcgccccc
      481 gtcggcggcg agaccctgcc cggccacgac ttcgcgatgc tcgccggcgg caagggcgcc
      541 aaccaggcgg tcgcgtgcgc gcgcatgggc gcgagcgtgg cgatggtcgg ccgcgtgggc
      601 atcgacgcca acggcgacgc gctcaaggcc ggcctcgcgg ccgacggcat cgacgcgcgc
      661 gaggtcaagg cgctcgcgtc cacgcatacc ggcgtcgcct cgatctgggt cgaggacgac
      721 ggccagaacc gcatcgtcct cgcgtcgggg gccaacgggc tgctcgatgc cgccgccgtc
      781 gcgcgcgccc atccgatgat cgacgccgcc gagatgctga tcgtccagct cgaggtgccg
      841 atgcccgccg tgcaggcggc cgtcgagcgc gcctggcgcg ccggcgtgcc ggtgctcatg
      901 aatccggcgc cggccgcgcc gctgcccgac gcgatgtggg gcatgatcgc catcctcgtg
      961 gtcaacgaga ccgaggccgc cgcgctgtcg ggcctgccgg tggtcgatcc cgcgaccgcg
     1021 ggcgccgcgg gtcgcgcgct gcgcgccagg ggccccgacc gggtcatctg cacgctcggc
     1081 ggccagggct gcgttgtcgt cgacgccgac ggcgcgcgcc acacgccggc gatggtggtc
     1141 aaggtcgtcg acacgaccgc cgccggcgac accttcatcg gcgcgctggc cgtcgcgctg
     1201 tgcgagggcc agccgctgga cgtcgcggtc gccttcggcc aggccgcgag cgcgctgtgc
     1261 gtgacgcggc gcggcgcgca ggtctcgatc ccctaccgcc gtcaggtcgc cctgcccgcg
     1321 gcgacgtcgc cgaccccgat cccgtcctga aaggccgcga tgaagcgcac cgtcctgctg
     1381 cacgccgagc tctccgaggt catcgcccgc ctcggccacg gcgacctgct cgtgatcggc
     1441 gacgcggggc tgccgatccc ggacgggccg cgccgcatcg acctggccgt ctcgcgcgac
     1501 gtgccgcgct tccacgacgt gctcgccgcc gtgctggcgg agatgaaggt cgagtcggcc
     1561 gtcgtggccg acgagctcgc cgggcacaac gccgtcgtca acatggagct gcgccgccag
     1621 ctgcacggca tggtgctcga gaccgtgccg cacgcggact tcaagtcgat gacgcgcacg
     1681 gcgcgcgccg tcgtgcgcac gggcgagtgc tcgccctatg ccaacgtgat cctgcgcgcg
     1741 ggcgtcgtgt tctgacgccc gcatcgccgg atcgacggca gactcagttc atgaagccgc
     1801 cgtcgttgcg cgcgacggcg ccgcccgccg cgcagctcgt gagctcgcgg ttgccgctgg
     1861 cggtcgactg gttgctctcc cacaccgcgg tcgagccgtt ccacttcacg tacttgtact
     1921 ggatcgcggc attggcgggc agcgccacgg tgccggtcca cggcacgttg gcgccggagc
     1981 cctggatcgc gagcgcgaag cccgatgccg gggtccagtt gccgagcgcg gactggttgc
     2041 cgacgacgta gaggttctgg ccgtagctcg tgttggcgtt ggcgatcgtg aacgtcaccg
     2101 cgcaggtcgt cgcgccgccg ccgccgccgc ccgagccggc gatcatctgg ccggtgtaga
     2161 tcgccaccgc cggcacggtg gagccgccgt cggccgcgac cgtgatcgcg gcgttgccgc
     2221 tcgcgtccac cgtcaccgag tcgctcgtgc acgcggtgcc ggccgcgttc ttgaggccgt
     2281 ggatcacgtt gcagtacgtg cccgcgggca gccccgtgta gaaggtgcgc gaccacgacg
     2341 agccggtgtt gttcagcgcc acgaagccca cgttgccgcg gctgaacgcg atctggttcg
     2401 cgttgccggt gacccagttg ccctgcgcct ggccgtgcgc ggcggagcgg aacttcacca
     2461 tgttggagat gtccgtccac cggtgcagga agtcccagtt cacgttgatc tgcgggacgc
     2521 cgttcgagta cgggctcgcg ctcggccggt cctggttcga gttggtgaag cggaagccgg
     2581 agtacagctg cgcctcgcca tagccctgcg ccagcatgaa gatgttggca aggtcgtagc
     2641 ggtgcgtgcc ctgggtgtcg ttggtgaccg agccggtgta gttgtcggcg tccagcgagc
     2701 tgccgtcgcg ctcgctgtcc cagttgttga ggaacacggt cgcgttcgtc ggctgcacga
     2761 agccccaggt gccgccccag ttgttccagg tgcccatcgc gctcgggatg gtcgacgggc
     2821 tggagccgtt ctggttgcgc cacgcgtcgc gcatcaggta ggtgaactgg aattcgttga
     2881 tcgtgccgat cgggaagtag ctcgaacgcg agacctcgcc gtcggggatg atctcctgcg
     2941 tcacccagat cggctcgccc aggctggtgg tcgggtgcgc ggccttcacc gcgctcatga
     3001 tcgcctgcag cgagctcgcg gcctggtgct tggcggcgtc gatgcgaaag ccgtcgacgc
     3061 ccagggccag caggtggttc aggtagttct cgacctggcc ctgcacgtag ctgctctcgg
     3121 tgtcgaggtc gggcagcccg cccaggcggc agttctgcac gtcccaggtg cccgacggcg
     3181 agttgtagtc ggaggactgg atcgcgcagt tggcgtggaa gtcgttcgcg ctgaagtagg
     3241 gataggcgag ggtcgacgag tcccaggtcg agccgtcggt ggcgacgccg ttgctcgcgc
     3301 tcgagccgtc ggccagctgg ttgacgacga tgtccgcgta cacgcgcacg ccggcggcgt
     3361 ggcaggtgtc gatcatcgac ttcagctgcg tctcgttgcc gaagcggctg gtcaggttga
     3421 cgtagttcac cggctggtag acgccccacc agccggtggc gttcttggac gcacccggcg
     3481 gcgagatctg cacgccgccg tagccctgcg gcccgagcca ctgggtgcat tcggtggcga
     3541 cgtcgttcca cgcccactcg aacatctgca ccgaggtgtc gttggggttg agggcggcgg
     3601 cgcccgcggg cgcggcgaag gcggcgccaa gggccgcggc cagcgcgccg agctgcagcg
     3661 cgcgagggaa gggtcggttc atctgtcttg tctcctggtg gcggccccag gcgggccggc
     3721 cgatgttcgt tctgatggct gggcgacgtc cttgcggccc tctcggtgag aggtcttgcc
     3781 gatctccggt gatgcgatag aagtagtttt agtacctgca aatcgaacca acaacgcggg
     3841 ttgacccttg atttgcgctc aatgacccat gtttttccgt acttttacgt cgagtacaaa
     3901 tcaatgtaat tacaaaacat gcattgcaca taaactcatc cttcgtgtat tttgaatact
     3961 t
//
LOCUS       DASVLN010000578         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_516265, whole genome shotgun sequence.
ACCESSION   DASVLN010000578 DASVLN010000000
VERSION     DASVLN010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516265"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VF453_13920"
     CDS             <1..765
                     /locus_tag="VF453_13920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7688805.1"
                     /translation="AVAVIDDHPDVARTLGMALERAGHRVIRYADGPSALAGMRETLP
                     EAAIMDTRLPGMNGFEVLGVMREDPRLRDVRVIGVSGAVDPALRGNGPRFDAFLMKPV
                     DIRQLLRLLGGGDGPPIRVLLVEDHSALRAVTTDLLRAERFEPVAVSSAAEALETAER
                     VRPQLLLCDMNLPDMPGLALIGRLQPQLRQWGTYVAVVTALPEDEIRSFARNAESLGV
                     DAFIAKPLTARWLRDVAARLQAAHGASAGRGPVVRR"
     gene            947..1393
                     /locus_tag="VF453_13925"
     CDS             947..1393
                     /locus_tag="VF453_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7688806.1"
                     /translation="MNAIASLDPRFSPLSADSFPDLLRRLARMADLPGLRMDEDIRVD
                     VTETDKDYFVKAELPGVAKEDVRVHVDGNYVAISAMAHPQKKEARKHDGERTLLHETH
                     DGMWSRGISLPREIEDKAADARFDNGVLSLRLPKRQPASGKTVAIK"
BASE COUNT          208 a          589 c          567 g          201 t
ORIGIN      
        1 gccgtggccg tgatcgacga ccacccggac gtcgcgcgca cgctcggcat ggcgctggag
       61 cgcgcgggcc accgcgtcat ccggtacgcg gacggcccgt ccgccctggc aggcatgcgc
      121 gagacgctgc cggaggcggc gatcatggac acccggctgc cggggatgaa cggcttcgag
      181 gtcctgggcg tgatgcgcga ggatccgcgg ctgcgcgatg tccgcgtgat cggcgtgtcg
      241 ggtgccgtcg acccggcctt gcggggcaac ggcccgcgct tcgatgcgtt cctgatgaag
      301 ccggtggaca tccggcagct gctgcgcctg ctggggggcg gcgacggccc gccgatccgc
      361 gtgctgctgg tcgaggatca ttcggcactg cgggccgtca ccacggacct gctgcgcgcg
      421 gagcgcttcg agcccgtggc cgtctcctcg gcggccgagg ccctcgagac cgccgagcgc
      481 gtccggccgc agctgctgct gtgcgacatg aacctgcccg acatgcccgg gctcgcgctg
      541 atcggccgcc tgcagccgca gctgcgccag tggggcacct acgtggcggt cgtgacggcg
      601 cttccggaag acgagatccg gtccttcgcc cgcaacgccg aaagcctcgg cgtcgacgcg
      661 ttcatcgcca agccgctcac cgcccgctgg ctgcgcgacg tggcggcgcg gctccaggcc
      721 gcgcacggcg cgtccgcagg ccgcgggccg gtcgtccggc gctgaggggt cccgcggggg
      781 cggcccgccg gcgtcgcgaa gtccacctgc agggctggcc cgccgcaacg tgcccggggc
      841 cgggccgcga ttgcgtttcc gcaattccgc ctccgccgcc aggctggacg atgccccgat
      901 tcgcgccccc gaggcgcgat gccacccacc atcaccggga gatcccatga acgccatcgc
      961 cagcctggac ccgcgcttca gccccttgtc cgccgactcg tttcccgacc tcctgcgccg
     1021 cctggcgcgc atggccgacc tgcccggcct gcgcatggac gaggacatcc gggtggacgt
     1081 caccgagacc gacaaggact acttcgtgaa ggccgagctg ccgggcgtcg ccaaggagga
     1141 cgtgcgcgtc cacgtggacg gcaactacgt cgcgatctcc gccatggcgc acccgcagaa
     1201 gaaggaggcc cgcaagcacg acggcgaacg cacgctgctg cacgagacgc acgacggcat
     1261 gtggtcgcgc ggcatcagcc tgccgcgcga gatcgaggac aaggccgccg acgcccgctt
     1321 cgacaacggc gtgctctcgc tgcggctgcc caagcgccag cccgcctcgg gcaagacggt
     1381 cgcgatcaag tgacgcactt cgcatcgccg cccgtggggc cgcccgccaa ggccctgcgc
     1441 gggatggccg agctcgagcg ccgggtccag gtcggcgcgc tcgcgctgga aggcgacctg
     1501 acgctgcccg agcgacggcg cggcctcgtg gtgctcgcgc atggctgcgg cgcggcacgc
     1561 cgcag
//
LOCUS       DASVLN010000579         2338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_461726, whole genome shotgun sequence.
ACCESSION   DASVLN010000579 DASVLN010000000
VERSION     DASVLN010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461726"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VF453_13930"
     CDS             <1..494
                     /locus_tag="VF453_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688807.1"
                     /translation="GARLVAVLWAEPAGWRLADASGDIDAACPGCDVAVRVRGGLLEV
                     STSDPGEAVARLVGWRFAYRGAKRDVLRLVAVRHELIWHGGGDRADPAGAVVDDVATT
                     DLLTGDKVDVIEGLRDGRPTRLEARSRVPLRQVIAFDQFGFDLRAAGAETRLVFERDF
                     RRR"
     gene            761..1690
                     /locus_tag="VF453_13935"
     CDS             761..1690
                     /locus_tag="VF453_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX7688808.1"
                     /translation="MAHPLSISSTVRLALLLLFVASALFVHFRGRARFGLLKALTDYT
                     VLLAPYNTLMVLFSRVKVKPFAPVTDFPELAPLQAHWEEIRAEALSLNESGRIAVATG
                     YNDLGFNSFFRSGWKRFYLKWYGETPASALAACPRTVELLSSIPSIKGAMFASLPPEG
                     RLVRHRDPYAGSLRYHLGLFTPNSPDCYIEVDGQRYFWRDGEAVVFDETFIHHAENTT
                     DHQRVILFCDVERPLHGGVLASINRWFANNIMAASASPNEVGERVGGINKFFQVAYKV
                     RLATKKLKSSNRRLYYLSKWVTIFGLIWLIFFA"
     gene            complement(1718..>2338)
                     /locus_tag="VF453_13940"
     CDS             complement(1718..>2338)
                     /locus_tag="VF453_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7688809.1"
                     /translation="AASAQPAAAGVDPVPAAIRVPRLPATGMLVPGAPLSAGERCDLV
                     AVRGETGWEVWADGEQPPGLRRHAIEALTLRSVSRLHVALLEDDARAAEAMVEAFRPL
                     DIEAESHASADSLLATIGRRRFDAYVLDWLLPDGDAARVVAAIRLHRPHVPVVVVTGA
                     LKADSEMERSLLELSVRWNFSTLDKPVRPMNLANAIKQLATAARAA"
BASE COUNT          312 a          842 c          839 g          345 t
ORIGIN      
        1 ggggcgcgcg gctggtcgcg gtgctctggg cggagccggc cggctggcgc ctggcggacg
       61 cgtccggcga catcgacgcc gcttgccccg gttgcgacgt cgcggtgcgg gtgcggggcg
      121 gcctgctcga ggtctcgacc agcgacccgg gcgaagcggt cgcccggctg gtcggctggc
      181 gcttcgccta ccgcggcgcc aagcgcgacg tgctgcggct ggtggccgtg cggcacgagc
      241 tgatctggca cggcgggggc gacagggcgg atcccgcggg cgccgtggtg gacgacgttg
      301 ccaccaccga cctgctgacc ggcgacaagg tggacgtcat cgagggtctg cgcgacggtc
      361 gcccgactcg gctggaggcc cgcagccgcg tgccgctgcg ccaggtcatc gcgttcgacc
      421 agttcggctt cgacctgcgc gcggccgggg cggagacccg gctggtgttc gaacgcgatt
      481 tccggcgccg atgagtcgtc ggggcggcag gcgccacggt cgggtccggt agtgccgatc
      541 caggccgatc caggccgatc caggccgatc ggggggcgag cgcatcgaac ggcatcgaat
      601 ggcacgggaa cccgcctcat tccgccgcgc gccggcatgc gggaatgtgg catcctcgac
      661 cgatccgcgt cccagagacc cgcgccgcgg acggcgacgt cgccaggcgt cagaatagcg
      721 ggttgacccg tccgcgcgcc cgcccggcgc gcacgcatcc atggcgcatc cactctccat
      781 ttcctcgacc gtccggctcg ccctgctgct gctgttcgtg gcctcggcgc tgttcgtgca
      841 cttccgcggc cgcgctcgct tcgggctgct gaaggcgctg acggactaca cggtgctgct
      901 ggcgccctac aacacgctga tggtgctgtt ctcgcgggtg aaggtgaagc ccttcgcgcc
      961 ggtgacggac ttccccgagc tcgcgccgct gcaggcgcac tgggaggaga tccgcgccga
     1021 ggcgctgagc ctgaacgagt ccggccgcat cgcggtggcg acgggctaca acgacctcgg
     1081 cttcaactcc ttcttccgct cgggctggaa gcgcttctac ctgaagtggt acggcgagac
     1141 gcccgcctcg gcgctggcgg cctgcccgcg gacggtcgag ctgctgtcga gcatcccctc
     1201 gatcaagggc gcgatgttcg cgtcgctgcc gcccgagggc cggctggtgc gccaccgcga
     1261 cccgtacgcc ggctcgctgc gctaccacct gggcctgttc acgccgaact cgcccgactg
     1321 ctacatcgag gtcgacgggc agcgttactt ctggcgcgac ggcgaggcgg tggtcttcga
     1381 cgagaccttc atccaccacg ccgagaacac gaccgaccac cagcgcgtga tcctgttctg
     1441 cgacgtcgag cgcccgttgc acggcggcgt tctggcgtcg atcaaccgct ggttcgcgaa
     1501 caacatcatg gcggcgtcgg cctcgcccaa cgaggtcggc gagcgcgtcg gcggcatcaa
     1561 caagttcttc caggtggcgt acaaggtgcg cctagcgacg aagaagctga agtccagcaa
     1621 ccggcggctg tactaccttt cgaagtgggt cacgatcttc gggctgatct ggctgatctt
     1681 cttcgcctga gtccgcgctc gcgacgccgc agccggctca ggcggcgcgc gcggccgtcg
     1741 cgagctgctt gatcgcgttg gccaggttca tcggacgcac cggcttgtcg agcgtggaga
     1801 aattccagcg caccgacagc tccagcagcg agcgttccat ctccgagtcg gccttgagcg
     1861 cccccgtcac cacgaccacc ggcacgtgcg gccggtgcag tcggatcgcc gcgaccacgc
     1921 gtgcggcgtc gccgtcgggc agcagccagt caaggacgta ggcgtcgaag cgccggcggc
     1981 cgatggtcgc gagcagcgag tcggccgacg cgtgcgactc cgcctcgatg tccagcggcc
     2041 ggaaggcctc gaccatcgcc tcggcggcgc gcgcgtcgtc ctccagcagg gcgacgtgca
     2101 gccgcgacac gctgcgcagc gtgagcgcct cgatcgcgtg ccggcgcagg ccgggaggct
     2161 gctcgccatc ggcccacacc tcccagcccg tttcgccgcg caccgcgacg aggtcgcagc
     2221 gctcccccgc cgagagcggc gctccgggca ccagcatgcc ggtggcgggc aggcgcggca
     2281 cgcggatcgc ggcgggcacc ggatccacgc ccgccgccgc cggctgtgcg gacgccgc
//
LOCUS       DASVLN010000580         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_461769, whole genome shotgun sequence.
ACCESSION   DASVLN010000580 DASVLN010000000
VERSION     DASVLN010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461769"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VF453_13945"
     CDS             <1..858
                     /locus_tag="VF453_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688810.1"
                     /translation="ELESRLGRHLAAKARHVSGLAEDDALQAARAEAAAGRQCTLGYW
                     SKPGESPASTAERYARAVERVADAGLPASVSIKVDLLGFDRALLAPVLRAARARDVRV
                     HFDAQGPDTITRTHALVEEACALGNDVSATLPSRFLRSRTDAERFIALRIPVRVVKGQ
                     GGDPEHPKLDPRRSYLELVDRLAGRAAFVGVATHDRRVAEPALRRLVAAGTPCGLEQM
                     RSLPRLDFIAQELGLATRVYVAYGHAGLPYAIGECLRRPAIVGWILRDLFLRHAPMTP
                     ESSRPPRQP"
     gene            855..>1813
                     /locus_tag="VF453_13950"
     CDS             855..>1813
                     /locus_tag="VF453_13950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7688811.1"
                     /translation="MSATPGPDTDDAPPAPVNVYTEPAFLRLVADVYHAGRGCRVRDF
                     AVDGGVYRLLEVEGVPQTTQRFIDLHESLGPPPLRRPALPRLARLEGVAGRAMQLERF
                     QQQAGWDALEGAPTVQWDGFRSWSDYLAQIQARRMTAEDLRRGRRLQERFPDLEFAAD
                     DPRADVLPTAFEWKTGRDLALAREPVFADARNVRFFEALRERGLLRASTLRGGGRLLA
                     LWLGAVHDGRWSGWIFAFNPDPALARYSLGQQLLYRMLEHSHGEGHREFDFSIGMEPY
                     KLKFATHVRPIGMAGRPSPAQRAGTAMRRWLVRHPRLHETARAL"
BASE COUNT          221 a          724 c          626 g          242 t
ORIGIN      
        1 gagctggagt cccggctcgg ccggcacctg gccgccaagg cgcgccacgt cagcggcctc
       61 gcggaggacg acgccctgca ggccgcgcgc gccgaggccg ccgccggccg ccaatgcacg
      121 ctcggctact ggtcgaagcc cggcgagtcg ccggcctcga ccgccgagcg ctacgcacgc
      181 gcggtcgaga gggtcgcaga cgccggcctg ccggcctcgg tgtcgatcaa ggtcgacctg
      241 ctcggcttcg accgcgccct gctcgctccc gtgctccgcg ccgctcgcgc gcgggacgtg
      301 cgcgtgcatt tcgacgccca gggcccggac accatcaccc gcacccacgc gctcgtcgag
      361 gaggcctgcg cgctcggcaa cgacgtctcc gcgaccttgc cctcgcgctt cctgcgctcg
      421 cgcacggatg ccgagcgctt catcgccctg cgcatccccg tgcgcgtcgt gaagggccag
      481 ggcggcgatc ccgagcatcc gaagctcgat ccacgccgct cctacctgga gctcgtcgac
      541 cggctcgccg gccgcgccgc cttcgtgggc gtcgcgacgc acgaccgccg cgtcgcggag
      601 ccggcgctcc ggcgcctggt ggcggcaggg acgccctgcg ggctcgagca gatgcgctcg
      661 ctgccgcggc tcgacttcat cgcgcaagaa ctgggcctgg cgacgcgggt gtacgtcgcc
      721 tacggccatg ccgggttgcc ctacgccatc ggcgaatgcc tgcgccggcc ggccatcgtc
      781 ggctggatcc tgcgcgacct cttcctgcgc cacgcgccga tgacgcccga atcctcccgc
      841 ccgccgcgcc agccatgagc gccacacccg gacccgacac ggacgacgcg ccccccgcgc
      901 ccgtcaacgt ctacaccgag cccgccttcc tccggctcgt cgccgacgtc tatcacgccg
      961 gccgggggtg ccgcgtgcgc gacttcgccg tcgacggcgg cgtgtaccgg ctgctcgagg
     1021 tcgaaggcgt gccgcagacg acgcagcgct tcatcgacct gcacgagtcc ctcgggccgc
     1081 cgccgctgcg ccggccggcc ctgccgcggc tggcgcggct ggaaggcgtc gccggccggg
     1141 cgatgcagct cgagcgattc cagcagcagg ccggctggga cgcccttgag ggcgcgccga
     1201 ccgtgcagtg ggacggcttc cgcagctggt cggactacct ggcgcagatc caggcacgcc
     1261 gcatgaccgc cgaagacctg cgccgcggcc ggcgcctgca ggagcgcttc ccggatctcg
     1321 agttcgctgc ggacgaccct cgcgccgacg tgctgcccac ggccttcgaa tggaagaccg
     1381 gacgcgacct cgcgctcgcg cgcgagcccg tgttcgccga cgcccgcaac gtccgcttct
     1441 tcgaggcgct gcgcgagcgc ggcctgctgc gcgcctcgac gctgcgtggc ggcggccggc
     1501 tgctcgcgct ctggctgggc gcggtgcacg acgggcgctg gagcggctgg atcttcgcgt
     1561 tcaacccgga tccggcactc gcgcgctatt cgctcgggca acagctgctg taccggatgc
     1621 tcgagcacag ccacggcgaa ggccatcgcg aattcgactt ctcgatcggc atggagccct
     1681 acaagctcaa gttcgcgacc cacgtgcgcc cgatcgggat ggccggcagg ccctcgcccg
     1741 cgcagcgcgc cggcacggcg atgcgccgct ggctggtgcg ccatccgcgc ctgcacgaga
     1801 ccgcgcgcgc cct
//
LOCUS       DASVLN010000581         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_191764, whole genome shotgun sequence.
ACCESSION   DASVLN010000581 DASVLN010000000
VERSION     DASVLN010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191764"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VF453_13955"
     CDS             <1..658
                     /locus_tag="VF453_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX7688812.1"
                     /translation="TALAAGRLVRRTVSLADGAVRQEEAGAPAALALGAGEVANTFGP
                     EYRLLLIGAGQLAEYLATMALFSGFAVTVCDPRVEQRAHWRVPGAALSGEMPDDLVSA
                     FAPDARTAVVALTHDPKLDDLALMEALHTPAFYVGAIGSRRNNEARRRRLAEHFGLAP
                     HELARLRGPIGVYIGSKTPPEIAVSVMAEVLAAKNGVVVPHAMQVAGAKAALEPAAGR
                     "
     gene            complement(680..889)
                     /locus_tag="VF453_13960"
     CDS             complement(680..889)
                     /locus_tag="VF453_13960"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX7688813.1"
                     /translation="MDTATQAAEDPRSDAEVDVGDPQALARWSEALGVEDEALQRAVQ
                     AVGPRVDRIKDYLGAGGMAGEQSGG"
     gene            complement(949..1125)
                     /locus_tag="VF453_13965"
     CDS             complement(949..1125)
                     /locus_tag="VF453_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688814.1"
                     /translation="MTFTPHAIEHEAAMTPPQAPMPETFFGAFDDAELELALKTVGPD
                     PRDLVHFLRSCRAQ"
     gene            1452..>2966
                     /locus_tag="VF453_13970"
     CDS             1452..>2966
                     /locus_tag="VF453_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX7688815.1"
                     /translation="MTVHTPPSPSSPPADVDQDRFELLVRSVRDYAIFMLDPQGRVLT
                     WNVGAEAIKGWKAEEIIGRSFEIFYPPEAVAAGWPKTELRLAAQRGRFEDLGWRVRKD
                     GSRIWANVVITALRGPGGDLVGFAKVTRDLSESRRQQEALRQSEEQLRLLVESVKDYA
                     LFMLDVNGCVLTWNAGAAAILGYSAGDVIGRHFSMFFAPDDRAAGRAQDELELARSTG
                     RAHAQGWRMRNDGSTFWADVRLSAVHDASGQLRGFAKVTQDLTEQRRLVELEGASRRM
                     TSFLAMLAHELRNPLAPIRNAASVLQMQPELPPVLERVRDVIDRQARHLTRLVDDLLD
                     VGRIATGKIVLRLEPMDYRDVVDAAIEAFAPAIAAKGQALELDLIRHPLPMTGDMTRL
                     VQVLQNVLGNAVRYVPAGGRIRVEMAIEHASVVTRVIDDGPGIAPEALERIFELFVQE
                     DAARLPSDSGLGIGLSLARALVAQHGGLITAQSAGLGRGATFTIRLPLVAPRAPA"
BASE COUNT          369 a         1084 c         1099 g          414 t
ORIGIN      
        1 gacggcgctc gcggcgggac gcctcgtgcg gcgcacggtc tcgctggccg acggcgccgt
       61 gcgccaggag gaggccggcg cgcccgcggc gctcgcgctc ggcgccggcg aagtggccaa
      121 caccttcggc cccgagtacc gcctgctgct gatcggcgcc ggccagctcg ccgaatacct
      181 cgccacgatg gccctgttca gcggcttcgc ggtgaccgtg tgcgacccgc gggtcgagca
      241 gcgggcgcac tggcgcgtcc ccggcgcggc gctttccggc gagatgcccg acgacctcgt
      301 ctccgcattc gcgcccgacg cccgcaccgc ggtggtcgcg ctcacgcacg atcccaagct
      361 cgacgacctc gcgctgatgg aggcgctgca caccccggcg ttctatgtcg gggcgatcgg
      421 cagccgccgc aacaacgagg cgcgccgacg gcgcctggcg gagcatttcg gcctcgcgcc
      481 gcacgagctc gcgcgcctgc gcggtccgat cggcgtctac atcggcagca agacgccgcc
      541 cgagatcgcc gtcagcgtga tggccgaggt gttggcggcc aagaacggcg tcgtcgtgcc
      601 gcacgcgatg caggtcgccg gcgcgaaggc cgcgctcgag ccggcagcgg ggcgctgacg
      661 ccgcgtgcag ccggggcctt cagccgccgg actgctcgcc cgccatgccg ccggcgccga
      721 gatagtcctt gatgcggtcg acgcgcgggc cgaccgcctg caccgcgcgc tgcagcgcct
      781 cgtcctcgac gcccagcgcc tcgctccagc gggcgagggc ctggggatcg ccgacgtcga
      841 cctctgcgtc ggagcgcggg tcttcggcgg cctgggtggc agtgtccatg gcagtctccg
      901 gtggatgccg cctttcgcgg caagtctcgg accggcagac gcgggcgttc actgcgcgcg
      961 gcagctgcgc aggaagtgga cgaggtcgcg cgggtcggga ccgacggtct tcagggcgag
     1021 ctcgagctcg gcgtcgtcga aggcgccgaa gaaggtctcg ggcatgggcg cctggggcgg
     1081 cgtcatggcg gcttcgtgtt cgatggcgtg gggcgtgaag gtcatggcag tctcttttcc
     1141 tgttctggga ggtgccggcc ggtgccggcg cggcgtgtcg tccgggtcgg ggcgcctggc
     1201 gccgccgtcg ttcgttggag aggatgttcg cagcgccgcc gcggccgcgc agccggacat
     1261 cgtcgatccc gcgtgtacgg cttttcctac aagcgggcga cggcggccgg cagcggcgtg
     1321 ggggctcctt gacgccgccc ccggggtcgc cccacgcccg ggggacgcct gccggcgcga
     1381 tactccgccg tctggggaag tgggtgcttc ccccctcggg ccggcggatt gccgggcccc
     1441 ggcctgccgc catgaccgtc cacacgccgc cctctccgtc cagcccgccc gccgacgtcg
     1501 accaggatcg cttcgagctg ctcgtccgaa gcgtgcgcga ctacgccatc ttcatgctgg
     1561 atccccaggg tcgcgtcctg acctggaacg tgggcgcgga ggcgatcaag ggctggaagg
     1621 ccgaggagat catcggccgc tcgttcgaga tcttctaccc gcccgaggcc gtcgccgcgg
     1681 gctggccgaa gaccgagctg cggctcgccg cgcagcgggg gcgcttcgag gatctcggct
     1741 ggcgcgtccg caaggacggc tcgcgcatct gggccaacgt cgtcatcacg gcgctgcgcg
     1801 ggcccggggg cgacctcgtc ggcttcgcca aggtcacgcg cgacctcagc gaatcccgtc
     1861 gccagcagga ggcgctgcgc cagagcgagg agcagctgcg cctgctcgtc gagtcggtga
     1921 aggactacgc cctgttcatg ctggacgtca acggctgcgt gctcacctgg aacgccggcg
     1981 ccgcggccat tctcggctac agcgcgggcg acgtgatcgg ccgccacttc tcgatgttct
     2041 tcgcgcccga cgaccgcgcc gccggccgcg cgcaggacga gctggagctg gcgcgcagca
     2101 ccggccgggc gcatgcccag ggctggcgca tgcgcaatga cggcagcacc ttctgggccg
     2161 acgtgcggct gagcgcggtc cacgacgcct cgggccagct gcgcgggttc gcgaaggtga
     2221 cgcaggacct gaccgagcag cgccgcctcg tcgagctgga gggcgccagc cgtcgcatga
     2281 ccagcttcct cgcgatgctc gcgcacgagc tgcgcaaccc gctcgcgccg atccgcaacg
     2341 ccgcgagcgt gctgcagatg cagccggagc tgccgcccgt gctcgagcgc gtgcgcgacg
     2401 tgatcgatcg ccaggcgcgc cacctcacgc ggctggtgga cgacctgctg gacgtcggcc
     2461 gcatcgccac cggcaagatc gtgctgcggc tggaaccgat ggactaccgc gacgtcgtcg
     2521 atgccgcgat cgaggcgttc gcccccgcga tcgccgcgaa ggggcaggcg ctcgagctcg
     2581 acctcatccg ccatccgctg ccgatgacgg gcgacatgac gcgcctcgtc caggtgctgc
     2641 agaacgtgct cggcaacgcg gtgcgctacg tgccggcggg cggccgcatc cgggtcgaga
     2701 tggcgatcga gcatgcgagc gtcgtcacgc gcgtgatcga cgacggcccc ggcatcgcgc
     2761 cggaggcgct cgagcgcatc ttcgagctgt tcgtccagga ggacgccgcg cgcctgccca
     2821 gcgacagcgg gctgggcatc ggcctcagcc tcgcgcgtgc gctggtggcg cagcacggcg
     2881 ggctcatcac cgcgcagagc gccggcctcg gccgcggcgc gaccttcacg atccggctgc
     2941 cgctggtggc cccgcgcgct ccggcg
//
LOCUS       DASVLN010000582         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_516500, whole genome shotgun sequence.
ACCESSION   DASVLN010000582 DASVLN010000000
VERSION     DASVLN010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516500"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1353
                     /locus_tag="VF453_13975"
     CDS             343..1353
                     /locus_tag="VF453_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7688816.1"
                     /translation="MTTIKDVAALAGVGLGTASRAMSGRGSVAPETRRRVEHAARSLN
                     FQPLRRARAEAGGSIGLVVPSFTGNFYAPLLQAVDAELRAAGRHLLVAGGRSEGDERR
                     RALDGIGFLLERRCAGVIVATNALSEADLHELRRRCPRVAFINRRAAGVERHCFDIDH
                     ELGGRLAARALLGRGHREIACIAGPADAADSRQRMAGFHAELARHGLAIPPARRAEGD
                     FSFAGGFAATRRLLEQAPAGWTALFCANDVMAMAAISRLARAGLAVPRDVSVLGFDDS
                     DIATYTTPQLTTVRIPVEQMAASACRFLLNECYGLAQPVAREFQPVLMWRDSVMPLEP
                     VR"
     gene            complement(1590..1958)
                     /locus_tag="VF453_13980"
     CDS             complement(1590..1958)
                     /locus_tag="VF453_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688817.1"
                     /translation="MNPIENPHNHDPHLTKDHDAGPPLDRLSPAERALAPEADAPYQS
                     ADAIADDGTYVGQAGGYFGAEAPAAETGEARVQPGSERGESTYGYPAGPGTGEPGGEH
                     RWGANRRQPQGEDGPPPRQA"
     gene            2042..2317
                     /locus_tag="VF453_13985"
     CDS             2042..2317
                     /locus_tag="VF453_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688818.1"
                     /translation="MTPSDTLQAAHAAREMGLSLPSPGYLFGAFFFSLAGFAAWRYGK
                     VVERPRTRWLGAAMMFYPYLVGRTWLLWLVGIALFAAMWWDGTNGGD"
BASE COUNT          296 a          861 c          908 g          393 t
ORIGIN      
        1 ggaatgagcg agcgcgccga ttgatggtcc atcacgatcg ctccaactgg gcgcccgtcg
       61 catgggtgtg gctgaccgag gcccgccttc tcgcttcgcg attggcgcag gcgtagatca
      121 tttcagctac accctctcag gaaatgctaa gaaatcgata atttgttcac aaggcgttca
      181 tgtcatgaca aacattggaa acgtgcttgg aaccgccacg gtcgacgggt tttcccgctg
      241 gccgcggcgc acggcgatgc tctactcgcg gtgctgctgg aatcgtttcc aaacggaatc
      301 ggttccaaca accacgatgc acaagaccgg gagacgttgg gcatgacgac gatcaaggat
      361 gtggcggcgc tggcgggcgt cggcctgggc acggcctcgc gcgcgatgtc ggggcgcggc
      421 tcggtggcgc ccgagacgcg ccggcgggtg gagcatgcgg cgcgctcgct gaacttccag
      481 ccgctgcgcc gggcgcgcgc cgaggcgggc ggctccatcg gcctggtggt gccgtccttc
      541 acgggcaatt tctacgcacc gctgttgcag gcggtggacg cggagctgcg agccgcgggc
      601 cgccacctgc tcgtggccgg cggccgaagc gagggcgacg agcgccggcg tgccctcgac
      661 ggcatcggct tcctgctgga acggcgctgt gcgggcgtca tcgtcgcgac caacgccctc
      721 agcgaggccg acctgcacga gctgcgccgc cgctgcccgc gcgtggcgtt catcaaccgg
      781 cgcgcggcgg gcgtcgagcg ccactgcttc gacatcgacc acgagctcgg cggccggctc
      841 gcggcgcgcg cgctgctcgg acgcgggcat cgcgagatcg catgcatcgc gggccccgcc
      901 gatgcggccg acagccgcca gcggatggcc ggcttccatg cggaactcgc gcgccacggc
      961 ctggcgatcc cgcccgcgcg tcgcgccgag ggcgacttca gcttcgcggg aggtttcgcg
     1021 gcgacgcgcc ggctgctgga gcaggccccg gcgggctgga cggccctgtt ctgcgccaac
     1081 gacgtgatgg cgatggcggc gatctcgcgc ctcgcgcgcg ccgggctcgc ggtgccgcgc
     1141 gacgtctccg tcctcggctt cgacgactcg gacatcgcca cctacaccac gccgcagctc
     1201 acgacggtgc gcatcccggt cgagcagatg gccgccagcg cctgccgttt cctgctcaac
     1261 gagtgctacg gcctggccca gcccgtcgcg cgcgagttcc agccggtgct gatgtggcgc
     1321 gactcggtga tgccgctgga gcccgtgcgc tgaaatcgcg gtgcgcatgg tggaccggtc
     1381 gccgcgccgc gggcccgact gtgtcgaaga cgatgcaatc cggccaaatg cgcgttacgc
     1441 gcgcccggcc caatgtcgcc tgtacctttg atggggtgca acccggcagc gctaccttcc
     1501 tctttcctcc ctggctgccg gcttcgacag cccggcgctc gcgccgggct tttttatcgg
     1561 ctcgccggat ggatcggcat gcgtgcggct catgcctggc gtggcggcgg accgtcctcg
     1621 ccctgcggct ggcggcgatt ggcaccccag cggtgctcgc cgccgggctc gccggtgccg
     1681 gggccggccg ggtagccgta cgtgctctcg ccgcgctcgc tgccgggctg gacgcgggcc
     1741 tcgccggtct ccgcggcggg cgcttcggcc ccgaagtagc cgccggcctg gccgacgtag
     1801 gtgccgtcgt cggcgatggc gtcggcgctc tggtacgggg cgtcggcttc cggggcgagc
     1861 gcgcgctcgg ccgggctgag ccgatccagc ggcggcccgg catcgtggtc cttggtcagg
     1921 tgcggatcgt ggttgtgggg attttcgatc gggttcatcg cggactcctg cgggcgacgc
     1981 cgggcgcggc gccagccggg ttccggcaag accggtgccg gcgaggcgat actggtgcac
     2041 catgacgcct tccgacacgc tccaggccgc ccatgccgcc cgggagatgg gcctgtccct
     2101 gccgagcccg ggctatctct tcggcgcgtt cttcttcagc ctcgcgggct tcgcggcgtg
     2161 gcgctacggc aaggtcgtcg agcgcccgcg cacgcgctgg ctgggggcgg cgatgatgtt
     2221 ctacccgtac ctggtcggcc gcacctggct gctgtggctg gtcggcatcg cgctgttcgc
     2281 ggcgatgtgg tgggacggca ccaacggcgg cgattgacag ccgccgcccg gctcaggcgc
     2341 ggacgagcag gttgaagcgc gccgtggcgc cgctggcggt cgcctcgagg tgcagcggca
     2401 ggcgctggcc ggtcgcgtgc tcgagcacca cgagccccga cggcggcgcg gcgccggc
//
LOCUS       DASVLN010000583         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_406876, whole genome shotgun sequence.
ACCESSION   DASVLN010000583 DASVLN010000000
VERSION     DASVLN010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406876"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1658
                     /locus_tag="VF453_13990"
     CDS             <1..1658
                     /locus_tag="VF453_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7688819.1"
                     /translation="VVARGRVEPAASPYRLTGVTIDVTERELALAALREATERSALAT
                     RAAGIGIWEWDADTGVSRWDEEMFLLRGRKPRPECPSFEEMYTWVHADDRALVDEQIR
                     LSREEDRPAYYEFRVLWPDGSCRWLASRSLPVRDERGRTRMRLGVNWDVSEAREAAAA
                     RQERALVLRESQAKSELLARMSHELRTPMNAVLGFTQLLLTEEAASAEAEAAATAPGD
                     AAGRAPPSVTSRRIRLEHIRSAGEHLLTLINDVLELSRLDGPEQRDVVLQPVQLGALV
                     AQTLPLVEGLARKHGVTVIAPPSGLQVRAETTRLKQVLLNLLTNAIKYNRPGGTVRVD
                     AVPEAGRVLLRVSDDGIGMTAAQQELAFEPFNRAGAERDEIEGTGIGLTIVRTLVERM
                     GGRITVASTPGVGSVFAVSLDAAAAAAPLAQPTRRVLYVEDNEVNVMIVAELVRRRGD
                     VEFHSAPDGATGIASAARLMPGLTLVDMQLPDIDGLEVLQRLRADPATAGLRVVALSA
                     NAIPADIQRALDAGFDDYWTKPLDFGVFGAAIERVFGAAGPVT"
     gene            complement(1743..>2031)
                     /locus_tag="VF453_13995"
     CDS             complement(1743..>2031)
                     /locus_tag="VF453_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX7688820.1"
                     /translation="DLREEPHDWPRQRRPVWLVDPPRPLREQAGTPLLDGRPLWLVAG
                     PERIEADWWEDLVARDYFIAQADDGALLWVYRFRFAPADGESGWYLQGRFG"
BASE COUNT          264 a          760 c          767 g          240 t
ORIGIN      
        1 acgtggtcgc gcgcggccgc gtcgagccgg cggccagtcc gtaccggctc acgggcgtca
       61 cgatcgacgt cacggagcgc gagctcgcgc tggcggcgct gcgcgaggcg accgagcgca
      121 gcgcgctggc cacgcgcgcc gcgggcatcg gcatctggga gtgggacgcc gacaccggcg
      181 tctcgcgctg ggacgaggag atgttcctgt tgcgcggccg caagccccgc cccgagtgcc
      241 cgtcgttcga ggagatgtac acctgggtgc atgccgacga tcgcgcgctc gtcgacgagc
      301 agatccggct gtcgcgcgag gaggaccggc ccgcctacta cgagttccgc gtgctctggc
      361 ccgacggcag ctgccgctgg ctcgcctccc ggtcgctgcc ggtgcgcgac gagcgcggcc
      421 gcacgcgcat gcgcctgggc gtcaactggg acgtgagcga ggcgcgcgag gccgccgcgg
      481 cgcgccagga gcgcgcgctg gtgctgcgcg agagccaggc caagtccgag ctgctcgcgc
      541 gcatgagcca cgagctgcgc acgccgatga acgcggtgct gggcttcacg cagctgctgc
      601 tgaccgagga ggccgcgagc gccgaggccg aggctgcggc gaccgcgccg ggcgacgccg
      661 cgggccgcgc gccgccctcc gtcacgagcc ggcgcatccg cctggagcac atccgctccg
      721 ccggcgagca cctgctgacg ctgatcaacg acgtgctgga gctctcgcgc ctggacggcc
      781 ccgagcaacg cgacgtcgtg ctgcagcccg tgcagctggg cgcgctggtc gcgcagacgc
      841 tgccgctggt cgaaggcctc gcgcgcaagc acggcgtcac cgtgatcgcg ccgcccagcg
      901 ggctgcaggt gcgggccgag acgacgcggc tgaagcaggt gctgctgaac ctgctcacca
      961 acgccatcaa gtacaaccgg cccggcggca cggtgcgcgt ggacgcggtg cccgaggccg
     1021 gccgcgtgct gctgcgcgtg agcgacgacg gcatcggcat gacggcggcg cagcaggagc
     1081 tcgcgttcga gcccttcaac cgcgccggcg ccgagcgcga cgagatcgag ggcaccggca
     1141 tcggcctgac catcgtgcgc acgctggtcg agcgcatggg cggccgcatc accgtcgcca
     1201 gcacgccggg cgtgggcagc gtgttcgcgg tctcgctgga cgcggccgcg gcggccgccc
     1261 ctctcgcgca gccgacgcgg cgcgtgctgt acgtcgagga caacgaggtc aacgtgatga
     1321 tcgtcgccga gctcgtgcgc cgccgcggcg acgtcgagtt ccacagcgcg cccgacggcg
     1381 ccaccggcat cgccagcgcc gcgcggctga tgcccgggct gacgctcgtc gacatgcagc
     1441 tgcccgacat cgacggcctg gaggtgctgc agcgcctgcg cgccgacccc gcgaccgccg
     1501 ggctgcgcgt ggtcgccctg tcggccaatg cgatccccgc cgacatccag cgcgcgctcg
     1561 acgccggctt cgacgactac tggacgaagc cgctggactt cggggtgttc ggcgcggcga
     1621 tcgagcgcgt gttcggggcg gccggcccgg tgacctgacc ggcgccgccc tgcggcccgc
     1681 gcccggggac atgccgggtc gatcgccgga ccgcctgccc ccccttcgcg gacgccccgg
     1741 aatcacccga accgcccctg caggtaccag ccgctctcgc cgtccgccgg cgcgaagcgg
     1801 aagcgataga cccacagcag cgcgccgtcg tcggcctggg cgatgaagta gtcgcgcgcg
     1861 acgaggtctt cccaccagtc ggcctcgatg cgctcgggcc cggcgacgag ccatagcggg
     1921 cggccgtcca gcagcggggt gccggcctgc tcgcgcagcg gccggggcgg atcgacgagc
     1981 cagacggggc ggcgctggcg cggccagtcg tgcggctcct cgcgcaggtc g
//
LOCUS       DASVLN010000584         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_30872, whole genome shotgun sequence.
ACCESSION   DASVLN010000584 DASVLN010000000
VERSION     DASVLN010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30872"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1266
                     /locus_tag="VF453_14000"
     CDS             100..1266
                     /locus_tag="VF453_14000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX7688821.1"
                     /translation="MKALTYHSSKDVRVEQVPDPVLVEPDDVVLRVTATAICGSDLHI
                     YRGKIPAMKAGDILGHEFMGIVEEAGPAVTRVRKGDRVVVPFTISCGECFFCARRLFA
                     ACETTNTGRGAIVNKKDTRPGAALFGYSHLYGGIPGGQAEFVRVPKANSGPLVIPDST
                     LADEQVLFLSDILPTGWMAAVNAGVGEGSTVAIFGAGPVGLMAAASARCLGASRIFMV
                     DHHPYRLDFARETYGVEPINFDEVDDPAEFIIQQTAGRGVDGTIDCVGFEGKGSALET
                     ALTTIKLEGSSGAVLRQAMAATRRGGIVSVPGVYAGWIHGFLFGDAFEKGLTLRGGQT
                     HTQAYMPELLDHISSGRLRPDVIITHRMPLADAARGYEIFEKKQEDCRKVVLTP"
     gene            1315..2109
                     /locus_tag="VF453_14005"
     CDS             1315..2109
                     /locus_tag="VF453_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7688822.1"
                     /translation="MNATTSTRPLAVVTGASSGIGLELARLAAREGHDLVVAADGDAI
                     HRAAEELRSGGADVQVVQADLATRAGCEQVLAALGGRPVAALMANAGHGLGEGFLDQK
                     FEDIEHLVDTNIMGTLMLVHAIGRGMRQRGEGRILLTGSIAGLMPGSFQAVYNASKAF
                     VDSFSFALRNELKDSGVTVTCLMPGPTETNFFEHAGLQDTQVGTDEKADPADVAKDGF
                     EAMKKGEQKIVSGWKNKLQAAMAHVTPDSVLAQQHRKMAEPGTAKH"
BASE COUNT          401 a         1036 c          928 g          355 t
ORIGIN      
        1 cggcaaccac gcgcggggcc gccccgcgcg acggccgcca cggccgcggt acgcggcgtg
       61 cctcgacgag cccaacgcaa ccccgctgga gcctcgtcga tgaaagcgct gacctaccac
      121 tcgtccaagg acgtccgcgt cgagcaggtg cccgaccccg tgctcgtcga gcccgacgac
      181 gtcgtgctgc gcgtgaccgc caccgcgatc tgcggctccg acctgcacat ctaccgcggc
      241 aagatcccgg cgatgaaggc cggcgacatc ctcggccacg agttcatggg catcgtcgag
      301 gaggccgggc ccgccgtcac gcgcgtgagg aagggcgacc gcgtggtcgt gccgttcacg
      361 atctcgtgcg gcgagtgctt cttctgcgcg cgccgcctgt tcgccgcctg cgagacgacc
      421 aacaccggcc gcggcgccat cgtcaacaag aaggacacgc ggcccggcgc cgcgctcttc
      481 ggctacagcc acctgtacgg cggcattccc ggcgggcagg cggagttcgt gcgcgtgccc
      541 aaggccaaca gcggcccgct ggtcattccc gactcgacgc tggccgacga gcaggtcctg
      601 ttcctctcgg acatcctgcc caccggctgg atggccgcgg tcaacgcggg cgtcggcgag
      661 ggctcgaccg tcgcgatctt cggcgccggc ccggtcggcc tgatggcggc cgcgtccgcg
      721 cgctgcctgg gcgcgtcgcg catcttcatg gtcgaccacc atccctaccg cctggacttc
      781 gcgcgcgaga cctacggcgt cgagccgatc aacttcgacg aggtcgacga ccccgccgag
      841 ttcatcatcc agcagacggc cggacgcggc gtcgatggca cgatcgactg cgtcggcttc
      901 gagggcaagg gctcggcgct ggagaccgcg ctgacgacga tcaagctcga gggctccagc
      961 ggcgcggtgc tgcgccaggc catggccgcg acgcggcgcg ggggcatcgt cagcgtgccg
     1021 ggcgtctacg ccggctggat ccacggcttc ctgttcggcg acgccttcga gaagggcctg
     1081 accctgcgcg gcggccagac ccacacgcag gcctacatgc ccgaactgct cgatcacatc
     1141 tcgagcgggc gcttgcggcc cgacgtgatc atcacccacc gcatgccgct ggccgacgcc
     1201 gcgcgcggct acgagatctt cgagaagaag caggaggact gccgcaaggt cgtcctgacg
     1261 ccctgagcct gcccgtccgt tcctccccct tccttcacct tcgaaagacc cgtcatgaac
     1321 gccaccacgt ccacccgtcc gctcgctgtc gtcaccggcg cctcctccgg catcggcctg
     1381 gagctcgccc gcctggccgc ccgggaaggc cacgacctcg tcgtcgccgc cgacggcgac
     1441 gccatccacc gcgccgccga ggagctgcgc tccggcggcg ccgacgtcca ggtcgtgcag
     1501 gccgacctcg ccacgcgcgc cggctgcgag caggtgctcg cggccctcgg cggccgcccc
     1561 gtcgcggcgc tgatggccaa cgcgggccac ggcctcggcg agggcttcct cgaccagaag
     1621 ttcgaggaca tcgagcacct cgtcgacacc aacatcatgg gcacgctgat gctggtgcac
     1681 gccatcggcc gcggcatgcg ccagcgcggc gagggccgca tcctgctgac cggctcgatc
     1741 gccggcctga tgccgggctc gttccaggcc gtctacaacg cgagcaaggc cttcgtcgac
     1801 tcgttctcgt tcgcgctgcg caacgaactc aaggactccg gcgtcaccgt cacctgcctg
     1861 atgcccggcc cgacggagac caatttcttc gagcacgccg gcctgcagga cacccaggtg
     1921 ggcaccgacg agaaggccga cccggcggac gtcgccaagg acggcttcga ggcgatgaag
     1981 aagggcgagc agaagatcgt ctccggctgg aagaacaagc tgcaggccgc catggcgcac
     2041 gtgacgcccg acagcgtgct cgcgcagcag catcgcaaga tggccgagcc gggcacggcc
     2101 aagcactgag ggcccatcgg ccggcccgcg ggtccgcgcc ttcgccgaac ggatgatcgc
     2161 cgaccacgaa ggctgggcgc agcacctcgg cgaagccgcc cgggtctccg ggctcgagcc
     2221 gccgcatccg cccgtcggcg gcgacccgac gcgcctcgtc gccggccgac ggccgcggcg
     2281 cgctccgcgt caccgcaagc gtcagccgtc gtcggtcctc ggaacgtcga cgacgggccc
     2341 cggagcttcg acgcagcttc ccgcagcgcg agcaagctcg cgccggcact cgcggcgtcg
     2401 cgctcgagcc gaggcgccgg ggccgcgggc gaggacgttc cttctccagg ccaggagcgt
     2461 ccgtcctttc ggcgaatcgt cctcgcgcgc ggaagcagcg atgccgccgg aaggcgagac
     2521 gtccgacctc cagggcggga cgtcgagcgc gccggagcga tcgccgacgc tcggagaacc
     2581 gtcgtcggag ctccagggcc cgtcgtcgga tttccagggt ccgtcgtcgg agcccggcgc
     2641 tgcaacgccc ggttgccgcg ggcgaggatc gcggcgcggg aagccgtcgt cggcccggca
     2701 ggctcaggcg ctcgcgccca
//
LOCUS       DASVLN010000585         5083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_245273, whole genome shotgun sequence.
ACCESSION   DASVLN010000585 DASVLN010000000
VERSION     DASVLN010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245273"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VF453_14010"
     CDS             complement(<1..270)
                     /locus_tag="VF453_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688823.1"
                     /translation="MTALLQLLDDHKLRLVHEDVAYAPIAGDAALGALLFALRLDEAG
                     AVAFEDARVADRPALHLALSTARQFAHALRRHAGRVERGSPALTGV"
     gene            complement(329..1603)
                     /locus_tag="VF453_14015"
     CDS             complement(329..1603)
                     /locus_tag="VF453_14015"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEX7688824.1"
                     /translation="MTVLVLGSGVIGTSIAYYLAKAGHDVTVVDRQPGPALETSFANA
                     GEISPGYASPWAGPGVPVKAIKWLLMHHSPLIIKPMLEPAMWRWGFAMLRNCTEARYR
                     VNKARMVRVAEYSRDCLKELRAETGISYDERALGTLQLFRTRKQLDGIGKDVEILDQY
                     GVPYQVLDRAGYLQYEPALAGVQDKFVGALRLPGDETGDCLLFTQRLAAMAEALGAKF
                     HFGTTITGLHADGRRVTGVDTDQGRLAADEVVLALGSHSPKWLRPLGIDIPVYPVKGF
                     SITVPIADPAAAPESTIMDETHKVAVTRLGDRIRVGGMAQLSGYDLALVEARRRTLEF
                     VLTDLFPRAGDVSKASFWTGLRPMTPDGTPVMGRTRLANLTLATGHGTLGWTMAAGTG
                     RAIADLLSGRPPEIDFDGLTAERYTAADGFAG"
     gene            1761..4559
                     /locus_tag="VF453_14020"
     CDS             1761..4559
                     /locus_tag="VF453_14020"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7688825.1"
                     /translation="MMKNTVSFRVLPLVAAAAVAASGLPFAAAAADGPAKVAKAAPAK
                     AKAMGSLPVQVREVEGVHEYRLPNGLQVLLIPDASKPTVTVNVTYRVGSRYEGYGETG
                     MAHLLEHLMFRSTKTMANVSTELSKRGMQFNGSTTADRTNYFETFPADPAQLAWALRT
                     EAERMTHANVIKKDLDSEMTVVRNEMESGENNPFRILMEKTNAAAFQWHAYGKDTIGA
                     RSDVENVNIPHLQAFYRKYYQPDNATLIVAGSFDPAKVLAEIAQDWSSTPKPTRVIEP
                     TYTVEPVQDGEREVTLRRVGGEQDLFATYHVPSMASPDIAALEVVANALGDTPNGRLH
                     KRLVEAGKATQAFAWVSRDADPGQFNVGLMLKKDDDLAAAQKIFLDTIESLKSEPITA
                     EELKRAQLQSDKAFDQLMADPQQLCVTLSESIAGGDWRLLFLQRDRIDALTLDDVNRA
                     AQTWLKASNRTLGRFIPTDAPDRVPYATRTDPEAQLKDFKPRADIGAGEVFDSSPAGL
                     DKRSQVFTLPNGMKLALLPKKTKGATVQVVFDLHFGSLDDLRGKRAAAVGAGIVIGTG
                     TETKTRAQLSDAFDALKTDWELNGGLGYGHAALVTKREQLQPALALLAEAMRKPAFPA
                     DEFEQGRRQTIQQLEKAADEPSAVASHALSAALHHYPADDPRYAPSIPEQIAELQGMK
                     LQDAADFYRSYWGADHADMAIVGDFDAAQVKAEVERLFGDWKSQHPFERWRNPVSDAK
                     GLHLVAEMKDKANAVVIGTLPMKLQDTDPDYPALVAAAHVLGGGEFGSRLNARIRIKD
                     GLSYGAGASLAASPFEPAGAVQFYAIYAPQNRARVEADFAEELARFAKDGITQAELDT
                     AKQAILAERRTARTDDGAVAHGWADKLDEGRTWAWSVEQDAKLQALTVDQVNAAIRKW
                     IVPADADWSMAGSFEAAK"
     gene            4556..4798
                     /locus_tag="VF453_14025"
     CDS             4556..4798
                     /locus_tag="VF453_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEX7688826.1"
                     /translation="MSHGAGDPLPPLPAAEPGRYRHYKGGEYELVGVARHSETLEALV
                     VYRPLYGEGALWVRPHAMFFEDVEVDGRRRPRFERI"
BASE COUNT          773 a         1850 c         1801 g          659 t
ORIGIN      
        1 gacgcccgtc aacgccggcg agccgcgctc gacgcgaccg gcgtggcggc gcagcgcatg
       61 cgcgaactgg cgtgcggtgg acagcgccag gtgcagcgcc ggccggtcgg cgacgcgcgc
      121 gtcctcgaaa gcgacggcgc cggcctcgtc caggcgcagc gcgaacaaca gcgctcccag
      181 cgccgcgtcg cccgcgatcg gggcgtaggc gacatcctcg tggacgaggc ggagcttgtg
      241 gtcgtcgagc agttgcagca gggcggtcat ggccgcgatt gtcgccgcgt cgcggatgcg
      301 gggagggcgc cgtccgctcg ccgacctttc agcccgcgaa cccgtccgcc gccgtgtagc
      361 gctcggccgt gagcccgtcg aagtcgatct ccggcgggcg acccgacagc aggtcggcga
      421 tcgcgcggcc ggtgccggcg gccatcgtcc agcccagcgt gccgtggccc gtggccagcg
      481 tcaggttcgc gagccgggtg cggcccatca ccggcgtgcc gtccggcgtc atcgggcgca
      541 ggcccgtcca gaagctcgcc ttcgagacgt cgccggcgcg cgggaagagg tcggtcagca
      601 cgaactccag cgtgcggcgg cgcgcctcga ccagcgcgag gtcgtagccc gagagctgcg
      661 ccatcccgcc gacgcggatg cggtcgccca ggcgcgtgac ggcgaccttg tgcgtctcgt
      721 ccatgatcgt cgattccggc gcggccgcgg ggtcggcgat cggcaccgtg atcgagaagc
      781 ccttgaccgg atagaccggg atgtcgatgc ccagcggccg cagccacttg ggcgaatggc
      841 tgccgagcgc gagcacgacc tcgtcggcgg cgaggcggcc ctggtcggtg tccacgcccg
      901 tcacgcgccg gccgtcggcg tgcaggccgg tgatcgtggt gccgaagtgg aacttcgcgc
      961 ccagcgcctc ggccatcgcg gccaggcgct gcgtgaacag caggcagtcg ccggtctcgt
     1021 cgccgggcag gcgcagggcg ccgacgaact tgtcctgcac gcccgcgagc gccggctcgt
     1081 actgcaggta gccggcgcga tcgagcacct ggtacggcac cccgtactgg tcgaggatct
     1141 cgacgtcctt gccgatgccg tccagctgct tgcgggtgcg aaacagctgc agcgtgccga
     1201 gcgcgcgctc gtcgtacgag atgccggtct cggcgcgcag ctccttcagg cagtcgcggc
     1261 tgtactccgc cacgcgcacc atgcgcgcct tgttgacgcg gtagcgcgcc tcggtgcagt
     1321 tgcgcagcat cgcgaagccc cagcgccaca tcgcgggctc gagcatcggc ttgatgatga
     1381 gcgggctgtg gtgcatcagc agccatttga tcgccttgac cggcacgccc gggccggccc
     1441 agggcgaggc gtagccgggc gagatctcgc cggcgttggc gaagctcgtc tcgagcgcgg
     1501 ggccgggctg gcggtcgacg accgtgacgt cgtgccccgc cttcgcgagg tagtacgcga
     1561 tcgacgtgcc gatcacgccg ctgccgagga cgaggacggt catgcgctgc cttccgccag
     1621 tgtagggatg cgccggcgcc gctcgtggcc gccggggcgc gggacgccgc aggataacct
     1681 gccggcgcag gggacggaac tcgcaagccg cggcaagctc tgacgccgat cgccgcgcgc
     1741 ggcatgccaa gatgcgctcc atgatgaaga acaccgtctc gttccgggtc ctgcccctcg
     1801 tcgccgcggc cgcggtcgcc gcctcgggcc ttccattcgc ggccgccgcg gccgacgggc
     1861 ctgccaaggt cgccaaggcc gcgccggcga aggcgaaggc gatgggcagc ctgccggtgc
     1921 aggtgcgcga ggtcgagggc gtgcacgagt accggttgcc gaacggcctg caggtgctgc
     1981 tgatccccga cgcgtccaag cccacggtca ccgtcaacgt gacctaccgc gtgggcagcc
     2041 gctacgaggg ctacggcgag accggcatgg cgcacctgct ggagcacctg atgttccgca
     2101 gcacgaagac gatggccaac gtctcgaccg agctctcgaa gcgcggcatg cagttcaacg
     2161 gcagcacgac ggccgaccgc accaactact tcgagacctt cccggccgat cccgcgcagc
     2221 tcgcctgggc gctgcgcacc gaggccgagc gcatgacgca cgccaacgtc atcaagaagg
     2281 acctcgacag cgagatgacc gtcgtccgca acgagatgga gtcgggcgag aacaacccct
     2341 tccgcatcct gatggaaaag acgaacgccg ccgccttcca gtggcacgcg tacggcaagg
     2401 acacgatcgg cgcgcgctcg gatgtcgaga acgtgaacat cccgcacctg caggccttct
     2461 accgcaagta ctaccagccg gacaacgcga cgctgatcgt cgccggctcg ttcgatcccg
     2521 cgaaggtgct cgcggagatc gcgcaggact ggtcatcgac gccgaagccg acgcgcgtga
     2581 tcgagccgac ctacaccgtc gagccggtgc aggacggcga gcgcgaggtc acgctgcgcc
     2641 gcgtcggcgg cgagcaggac ctgttcgcga cctaccacgt cccgtcgatg gccagccccg
     2701 acatcgcggc gctcgaggtg gtcgccaacg cgctcggcga cacccccaac ggccggctgc
     2761 acaagcgcct cgtcgaggcc ggcaaggcca cgcaggcgtt cgcgtgggtc tctcgcgacg
     2821 ccgatccggg ccagttcaac gtcggcctga tgctgaagaa ggacgacgat ctcgccgcgg
     2881 cgcagaagat cttcctcgac accatcgaga gtctgaagtc ggagccgatc acggccgagg
     2941 agttgaagcg cgcgcagctg cagtccgaca aggccttcga ccagctgatg gccgacccgc
     3001 agcagctgtg cgtgacgttg tccgagtcca tcgccggcgg cgactggcgc ctgctgttcc
     3061 tgcagcgcga ccgcatcgac gccctgacgc tggacgacgt caaccgcgcc gcgcagacgt
     3121 ggctcaaggc gtcgaaccgc accctcgggc gtttcatccc gaccgacgcg cccgaccgcg
     3181 tgccctatgc gacgcgcacc gatcccgagg cgcagctcaa ggatttcaag ccgcgcgcgg
     3241 acatcggcgc aggcgaggtc ttcgactcga gcccggcggg gctggacaag cgcagccagg
     3301 tcttcacgct gcccaacggc atgaagctcg cgctgctgcc caagaagacg aagggcgcca
     3361 cggtgcaggt ggtcttcgac ctgcacttcg gctcgctgga cgacctgcgc ggcaagcgcg
     3421 ccgcggccgt cggcgcgggc atcgtgatcg gcacgggcac ggagacgaag acgcgcgccc
     3481 agctgtccga cgccttcgac gcgctcaaga ccgactggga gctcaacggc ggcctcggct
     3541 acggccacgc cgcgctggtg acgaagcgcg agcagctgca gccggcgctc gcgctgctgg
     3601 ccgaggcgat gcgcaagccc gcgttcccgg ccgacgagtt cgagcagggc cggcgccaga
     3661 cgatccagca gctcgagaag gccgccgacg agcccagcgc cgtcgctagc cacgcgctgt
     3721 cggccgcgct gcaccactat ccggccgacg acccgcgcta cgcgccctcg atccccgagc
     3781 agatcgccga gctgcagggc atgaaactgc aggacgcggc cgacttctac cgcagctact
     3841 ggggcgccga ccacgccgac atggccatcg tgggcgactt cgacgccgcc caggtcaagg
     3901 ccgaggtcga gcgcctgttc ggggactgga agtcgcagca cccgttcgag cgctggcgca
     3961 acccggtctc cgacgccaag gggctgcacc tggtcgccga gatgaaggac aaggccaacg
     4021 ccgtcgtgat cggcacgctg ccgatgaagc tgcaggacac cgatcccgac tacccggcgc
     4081 tcgtcgccgc ggcgcacgtg ctgggcggcg gcgagttcgg ctcgcgcctg aacgcgcgca
     4141 tccgcatcaa ggacgggctg tcctacggcg ccggcgcgag cctcgcggcc tcgccgttcg
     4201 agccggcggg cgcggtgcag ttctacgcga tctacgcgcc gcagaaccgc gcccgggtcg
     4261 aggccgactt cgccgaggaa ctcgcccgct tcgcgaagga cggcatcacg caggccgagc
     4321 tcgacaccgc caagcaggcg atcctcgccg agcgccgcac cgcgcgcacc gacgacggcg
     4381 cggtggcgca cggctgggcc gacaagctcg acgagggccg cacctgggcg tggtcggtcg
     4441 agcaggacgc gaagctgcag gcgctgaccg tcgaccaggt caacgcggcc atccgcaagt
     4501 ggatcgtgcc ggcggacgcc gactggtcca tggccggctc cttcgaggcc gccaagtgag
     4561 ccacggcgcc ggcgacccgc tgccgccgct gccggcggcc gagcccggcc gctaccgcca
     4621 ctacaagggc ggcgagtacg agctggtcgg cgtcgcgcgc cacagcgaga cgctggaggc
     4681 gctcgtcgtc taccggccgc tctacggcga gggcgcgctg tgggtgcggc cgcacgcgat
     4741 gttcttcgag gacgtcgagg tggacggccg gcgccggccg cggttcgagc ggatctgacg
     4801 ggttgccgcc cgcctgcgcg cccggccggc cccggccgcc tggcgacggc tgctcgtcga
     4861 ttcatcccac catttcgtcg gtcggtcgca tccggcgttg cgccggcggg aggcctcgat
     4921 atgctggcgc cactcgtcgg atccggcctg cgcgcgcgca ccggatccga tgcgccgcgc
     4981 ccgcaaaacg atcccgagga gctcgccccg atgacgtccc gccgccccgc ctcgtcccct
     5041 ttcccggtcc gcccggcgac cctcgcggcg ctggccgcgg ccg
//
LOCUS       DASVLN010000586         7691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_406930, whole genome shotgun sequence.
ACCESSION   DASVLN010000586 DASVLN010000000
VERSION     DASVLN010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406930"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2144)
                     /locus_tag="VF453_14030"
     CDS             complement(<1..2144)
                     /locus_tag="VF453_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7688827.1"
                     /translation="MARMSRLLGAACLGAAVSAALGAAVVTGEYAPVHRSPAHTPSAS
                     HGRVIVKLKARATVMSAARVAQHGGPQVASAMGSRLGLSLHDGRVLGERTQVIQADGV
                     DSATLAATLAQDSEVEWVQVDQRRYVQAAPVNDPLYPDNQTTTTPVAGQWYLRTPNAA
                     AEDSGGLLNVSGINIEPAWAITHGSGAVIVADVDTGITSHPDIVGSAPGAADSKVFAS
                     AFSGTTASTAYGYDFVGYAAGTETGYTTTDGELTANDGGLADPDPSDPGDWITAAEDA
                     GTVDNKEFKGCGADGGGPVDSSWHGTQTAGILAAATNNGTGMAGAAYDTKLVPVRVLG
                     KCGGYDSDIIAGALWAAGLLTDTSVPANAHPARVINMSLGGKGTCGSAYVDGLTRLRN
                     AGVVVVAAAGNGDAKAGGVAVVTPANCVPAQSDTDQTPIVIAVGALRNAGDKVGFSDL
                     GPEVAISAPGGNCVNTSSSDACLFPILTTSNTGTTGPGSPTYSTSFGDPSLGTSFAAP
                     MVSGTVALMLAVAPNLTNAQIVNLLQTTAREFPTTGGSAGTPMCSSTITTGQLECYCT
                     KATCGAGMLDAGRAVTAAAALAVTTAVISNSEDTWSIAPGGSLTINGSDSVANPGRTI
                     ASYAWSINSGSTAASLGATSGPSVTLTGLSDGTAVVQLVVTDSNGQTSTTTKTVTVGT
                     ASSGGGSGGGGSSGGGGGGGAANPAWLLALALA"
     gene            complement(2385..3383)
                     /gene="gap"
                     /locus_tag="VF453_14035"
     CDS             complement(2385..3383)
                     /gene="gap"
                     /locus_tag="VF453_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7688828.1"
                     /translation="MTIKIGINGFGRIGRMVFRAAVQNFQDVEVVAINDLLEPEYLLY
                     MLKHDSVHGRFKGEASVDNGTLIVNGKRIRLTAVKDPAELKWGEVGADVVVESTGLFL
                     TKETCQKHLDAGAKKVIMSAPSKDDTPMFVYGVNDKSYAGQAIISNASCTTNCLAPVA
                     KVLNDAFGIKRGLMTTVHATTATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPELNKKLTGMSFRVPTSDVSVIDLTVELNKEASYEDICNAMKAASEGAMKGVLGYT
                     DEKVVSTDFRGEARTSVFDADAGISLDKTFVKVVAWYDNEWGYSNKVLEMARVVAG"
     gene            complement(3453..5465)
                     /gene="tkt"
                     /locus_tag="VF453_14040"
     CDS             complement(3453..5465)
                     /gene="tkt"
                     /locus_tag="VF453_14040"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7688829.1"
                     /translation="MANAIRALAMDAVQAANSGHPGAPMGMADIAVALWSRHLRHNPS
                     NPHWADRDRFVLSNGHGSMLQYALLHLTGYDLPIDELKNFRKLHSRTAGHPEVDVTPG
                     VETTTGPLGQGLANAVGMALAEKLLASEFNRPGHDVVDHMTYVFLGDGCMMEGISHEA
                     CALAGAWRLNKLVAIYDDNGISIDGHVVPWFIDDVRQRFVAYGWDVIDEVDGHDIDLV
                     DAAIAEARKSALRPTLIICKTVIGKGSPNRAGTSKAHGEALGADEVAATRKALGWNHA
                     PFEVPAEVYAAWDARKSGAQAESEWNARFDAYKAHFPELAAELVRRMAGELPANFAEI
                     AAEAVGAAHDKAETVATRKASQIALEQFTAALPELLGGSADLTGSNLTNTKSTPALRF
                     DDEGAVVRDAEGRQGRHINYGVREFGMAAIMNGVALHGGYVPYGGTFLTFSDYSRNAI
                     RMAALMKRRVVHVFTHDSIGLGEDGPTHQSVEHAASLRLIPNLDVWRPADTTETAVAW
                     TMALVQKHRPSALLLSRQNLPYLPKTDADVITQGAYVLAEPAEAGLDKKARIVLMASG
                     SEVHLAMQAQIELAKLRIPTRVVSVPSTSVFDRQPEDYKRRVLPDGVPRVAIEAGVTD
                     GWWKYGCAAVVGLDRFGESAPGPELFKLFGFTTDNVVAVARKVLAR"
     gene            5657..6340
                     /locus_tag="VF453_14045"
     CDS             5657..6340
                     /locus_tag="VF453_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7688830.1"
                     /translation="MKALILAAGRGERMRPLSDRCPKPLLPVRGKPLIEWHLEALARA
                     GVREVVVNTAWLEEQFEPALGDGARWGLKIAYSMEGRDHGGALETAGGIAKALPLLGD
                     AFWVVSGDVFLPGFAFAGADAEEFVASGALGQLWLAPNAPHHPGGDFGIDAHGLASRE
                     ARPKRTWASVGLFRAALFEDIVPGTALALRPKLEFAIDRGALRAKPWDGAWTDVGTPE
                     RLAALQDAG"
     gene            complement(6375..7358)
                     /locus_tag="VF453_14050"
     CDS             complement(6375..7358)
                     /locus_tag="VF453_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7688831.1"
                     /translation="MQYRRLGRAGLQVSQLSLGSWVTYANQVDVSAAVEMMAAAFDAG
                     VNFFDNAEAYAGGKSEEVMGEALRKLGWPRLNYVVSTKFFWGLPDPKGVSVNRRNTLN
                     RKYLMQAIDGSLQRFGLEHLDLVYCHRGDPHTPVEETVRAMSDMITQGKALYWGTSEW
                     TASDIRAAWEIAERHHLHKPVVEQPQYHLFHRAKVEQEYARLYEDIGLGLTTWSPLAS
                     GLLTGKYRQGIPTGSRGAMQNMAFLHDQLTDQAKNAAVAKLEPIARELGGTTAQLAIA
                     WAARNPHVSSVITGASSVGQLQDNLQAAALVDKLTPEVMARIDEVTKALSA"
     gene            7462..>7691
                     /locus_tag="VF453_14055"
     CDS             7462..>7691
                     /locus_tag="VF453_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HEX7688832.1"
                     /translation="MVTTHDTHRTAAPRLAVIGGGPVGLALALLARERLPAWSVSVFD
                     TRPADRDVSGDPRTLALSLGSVQLLERLRAWPA"
BASE COUNT         1053 a         2646 c         2720 g         1272 t
ORIGIN      
        1 gcgagcgcga gcgccagcag ccacgccggg ttcgcggcgc cgccgcctcc gccaccgccg
       61 ctgcttccac cgccgcctga gcctccgccg gacgacgcgg tcccgacggt gacggtcttc
      121 gtcgtcgtgg aggtttgccc attcgaatcg gtgacgacca gctggacgac ggcggtcccg
      181 tcggacagtc cggtcagcgt cacgctggga ccggatgttg cgccaagcga cgctgcggtc
      241 gatcccgaat tgatcgacca agcgtagctc gcgatcgtac ggccgggatt ggctacggag
      301 tccgaaccgt tgatcgtcag cgatccgccg ggcgcgatgc tccacgtatc ctcggaattc
      361 gagatcacgg ccgtggtcac cgccagcgcc gccgcggccg tcaccgccct cccggcgtcg
      421 agcatgccgg caccgcacgt cgccttcgtg cagtagcact cgagttgccc cgtcgtgatg
      481 gtcgacgagc acatcggagt acctgcagaa ccacccgtcg tggggaattc tcgtgctgtc
      541 gtctggagta gattgacgat ctgcgcgttg gtgaggttcg gcgccaccgc aagcatcagg
      601 gcgacggtac ctgagaccat cggtgctgcg aagctggtgc cgagactcgg atccccgaag
      661 ctcgtgctgt acgttggcga tccggggccg gtggtgccgg tgttgctggt cgtcagaatc
      721 gggaacaggc acgcatcgct ggacgacgtg ttcacgcaat tgccgcccgg cgcagagatc
      781 gcgacctccg ggcccaggtc ggagaaaccg accttgtccc cggcattgcg caacgcgccg
      841 accgcgatca cgatgggcgt ctgatcggtg tcggactgtg ccggaacgca attggccggc
      901 gtgacgacgg cgacgccgcc cgccttggcg tcaccgttgc ccgctgcagc gacgacgacg
      961 acaccagcat tgcgcaggcg cgtcaggcca tcgacatagg cgctaccgca ggttcccttc
     1021 ccgcctaggc tcatgttgat gacgcgcgcc ggatgagcat tggcgggaac agacgtgtca
     1081 gtgagcaggc cggccgccca tagcgcgccg gcaatgatgt cagagtcgta gccgccgcat
     1141 ttgccgagga cacgaaccgg aacgagcttg gtgtcgtagg cggcacctgc catgccggtg
     1201 ccgttgttgg tggccgccgc caggatgcct gcggtctggg taccgtgcca actgctgtcc
     1261 acgggtccgc caccgtcggc gccgcagccc ttgaactctt tgttgtcgac ggtgccggca
     1321 tcctccgcgg cggtgatcca gtcgccgggg tcggatgggt ccgggtcggc caatccaccg
     1381 tcgttggcgg tgagttcgcc gtctgtggtg gtgtagcccg tctcggttcc cgcggcgtag
     1441 ccgacaaagt cgtagccgta cgccgtcgac gccgtggtgc cgctgaacgc agaggcgaaa
     1501 accttgctgt cggcggcacc aggcgcgctg ccgacaatgt ccggatgact ggtgatgccg
     1561 gtgtcaacgt cggcgacaat gacggcgcca gagccgtgcg tgatcgccca cgccggctcg
     1621 atgttgatgc ccgagacgtt gagcaggccg cccgagtcct cggccgccgc gttgggcgta
     1681 cgcaggtacc actggcccgc taccggcgtg gtcgtggtct ggttgtccgg atacaagggg
     1741 tcgttgacgg gcgcggcttg cacatagcgg cgctggtcga cctgaaccca ctcgacttcg
     1801 ctgtcctggg cgagcgtcgc ggccaacgtg gccgagtcga cgccgtcggc ttggatcacc
     1861 tgcgtccgct cgccaagcac cctcccgtcg tgaaggctca gcccgagccg cgatcccatc
     1921 gcggacgcga cttgcggtcc gccgtgctgc gcaacccggg cggcggacat cacggtcgcg
     1981 cgcgccttga gcttgacgat cacccgccca tggctcgcag acggcgtgtg cgcgggcgac
     2041 cggtgcaccg gcgcgtactc gcccgtgacc accgccgcgc cgagcgccgc gctcaccgcg
     2101 gcgcccagac aggctgctcc cagcagccga ctcatgcgtg ccatcgcact ctccgaaaag
     2161 tgaggatccc gggcccgttc gcgtctcccg atcgtcgagc agtctagtgg agacgccttt
     2221 tcatccgcgt tgccggattg cgtcccgccc gacaagctga cggcatgttt gcgaacggac
     2281 gtgagcgcgg agcatatccc gcgcccgtgc ggcagcctgt cgggacgcca aagaaaacgg
     2341 cccgcgcgcg gcgggccgtt gtcgacggac gacgctgcgg ccggtcagcc ggcgacgacg
     2401 cgggccatct cgagcacctt gttcgagtag ccccactcgt tgtcgtacca ggccacgacc
     2461 ttgacgaagg tcttgtccag cgagatgccg gcgtcggcgt cgaacaccga ggtgcgcgcc
     2521 tcgccgcgga agtcggtcga gacgaccttc tcgtcggtgt agccgagcac gcccttcatc
     2581 gcgccctcgg acgcggcctt catcgcgttg cagatgtcct cgtagctggc ttccttgttc
     2641 agctccacgg tcaggtcgat caccgagacg tcggaggtcg gcacgcggaa ggacatgccc
     2701 gtgagcttct tgttcagctc ggggatcacc acgccgacgg ccttggcagc gccggtcgac
     2761 gacgggatga tgttctcgag gatgccgcgg ccgccgcgcc agtccttgtt ggacgggccg
     2821 tcgacggtct tctgcgtcgc ggtcgtcgca tgcaccgtgg tcatcaggcc gcgcttgatg
     2881 ccgaacgcgt cgttgagcac cttggcgacg ggcgccaggc agttggtcgt gcacgaggcg
     2941 ttcgagatga tggcctggcc cgcgtagctc ttgtcgttga cgccatagac gaacatcggc
     3001 gtgtcgtcct tggagggcgc cgacatgatg accttcttcg cgcccgcgtc gaggtgcttc
     3061 tggcaggtct ccttggtcag gaacaggccc gtcgactcga cgacgacgtc cgcgccgacc
     3121 tcgccccact tcagctcggc cggatccttg acggcggtca ggcggatgcg cttgccgttg
     3181 acgatcagcg tgccgttgtc gaccgaggcc tcgcccttga agcggccgtg gaccgagtcg
     3241 tgcttgagca tgtacagcag gtactccggc tcgagcaggt cgttgatcgc cacgacctcg
     3301 acgtcctgga agttctgcac ggcggcacgg aacaccatgc gcccgatgcg gccgaagccg
     3361 ttgatgccga tcttgatggt catgtcgatg tctctcgttg aagtgaagga atcgttgcgg
     3421 gggcgcgggc cccgagtctg cccgcgcgcc ggttaccgcg cgagaacttt tcgtgccacg
     3481 gcgaccacgt tgtccgtcgt gaagccgaac agcttgaaca gctcggggcc gggcgccgac
     3541 tcgccgaagc ggtcgaggcc gaccaccgcc gcgcagccgt acttccacca gccgtcggtc
     3601 acgccggcct cgatggccac gcgcggcacg ccgtcgggca gcacccggcg cttgtagtcc
     3661 tcgggctggc ggtcgaacac gctggtcgag ggcaccgaca ccacgcgcgt cgggatcctg
     3721 agcttcgcca gctcgatctg ggcctgcatc gccaggtgca cctcggagcc cgaggccatc
     3781 agcacgatcc tcgccttctt gtcgaggccg gcctccgcgg gctcggccag cacgtaggcg
     3841 ccctgggtga tgacatcggc gtcggtcttg ggcaggtagg gcaggttctg gcgcgagagc
     3901 agcaacgcgc tggggcggtg cttctgcacg agggccatcg tccacgcgac ggcggtctcg
     3961 gtcgtgtcgg ccggacgcca gacgtcgagg ttggggatca ggcgcaggct cgcggcgtgc
     4021 tcgacggact ggtgcgtcgg gccgtcctcg ccgaggccga tcgagtcgtg cgtgaacacg
     4081 tgcaccacgc ggcgcttcat gagcgcggcc atgcggatcg cgttgcggct gtagtcgctg
     4141 aaggtcagga acgtgccgcc gtagggcacg tagccgccgt gcagcgcgac accgttcatg
     4201 atcgcggcca tgccgaactc gcgcacgccg tagttgatgt ggcggccctg gcggccctcg
     4261 gcgtcgcgca cgacggcgcc ctcgtcgtcg aatcgcagcg cgggcgtgct cttcgtgttg
     4321 gtgaggttcg agccggtcag gtcggccgag ccgcccagca gctcgggcag cgcggcggtg
     4381 aactgctcca gcgcgatctg gctcgccttg cgggtggcca ccgtctccgc cttgtcgtgg
     4441 gcggcgccga cggcctcggc ggcgatctcc gcgaagttgg cgggcagctc gccggccatg
     4501 cggcgcacga gctccgcggc gagttcgggg aaatgcgcct tgtaggcgtc gaagcgggcg
     4561 ttccactcgc tctcggcctg cgcgcccgac ttccgggcgt cccaggcggc gtagacctcg
     4621 gccggcacct cgaacggcgc gtggttccag cccagcgcct tgcgggtcgc ggcgacctcg
     4681 tcggcgccca gcgcctcgcc gtgcgccttg gacgtgccgg cgcggttggg cgaacccttg
     4741 ccgatgacgg tcttgcagat gatcagcgtc gggcgcagcg cgctcttgcg ggcctcggcg
     4801 atcgcggcgt cgacgaggtc gatgtcgtgg ccgtcgacct cgtcgatcac gtcccagccg
     4861 taggcgacga agcgctggcg cacgtcgtcg atgaaccagg gcacgacgtg gccgtcgatc
     4921 gagatgccgt tgtcgtcgta gatcgcgacc agcttgttca ggcgccacgc gcccgcgagc
     4981 gcgcaggcct cgtggctgat gccttccatc atgcagccgt cgccgaggaa gacgtaggtc
     5041 atgtggtcga cgacgtcgtg gccggggcgg ttgaattcgg aggcgagcag cttctccgcg
     5101 agcgccatgc ccacggcgtt ggccaggccc tggcccagcg ggccggtggt cgtctcgacg
     5161 cccggagtga cgtcgacctc cggatggccc gcggtgcggc tgtggagctt gcggaagttc
     5221 ttcagctcgt cgatgggcag gtcgtagccg gtcaggtgca acagcgcgta ctgcagcatc
     5281 gagccgtggc cgttggacag cacgaagcgg tcgcggtcgg cccagtgcgg gttggacggg
     5341 ttgtggcgca ggtggcggct ccacagcgcg acggcgatgt cggccatgcc catcggcgcg
     5401 ccggggtggc ccgagttggc ggcctgcacc gcgtccatcg ccagcgcgcg gatcgcgttg
     5461 gccatcagcg tgggcgtggc ctgcaccggg gcggcggtgg cggcttcggg ggcgacgtcg
     5521 gcggtggtct cggacatgga ggcgtctctt tcggcggggg caactggcgg aacccctaat
     5581 tttgtcagaa tcgcgggcac tgccctcaaa ccgcgacccg atcgcccccc gaacgcgcca
     5641 gaagcgcccg ccatccatga aggccctgat cctcgccgcc ggtcgcggcg aacgcatgcg
     5701 tcccctgagc gaccgctgcc ccaagccgct gctgccggtg cgcggcaagc cgctgatcga
     5761 gtggcacctc gaggcgctcg cgcgcgcggg cgtgcgcgag gtggtcgtca acacggcttg
     5821 gctggaggag cagttcgagc ccgcgctcgg cgacggcgcg cgctggggct tgaagatcgc
     5881 ctactcgatg gaaggccgcg accacggcgg cgcgctggag acggcgggcg gcatcgccaa
     5941 ggcgctgccg ctgctgggcg acgcgttctg ggtcgtctcg ggcgacgtgt tcctgcccgg
     6001 cttcgcgttc gcaggcgccg atgccgagga gttcgtcgcc agcggcgcgc tgggccagct
     6061 ctggctggcg ccgaacgcgc cccatcatcc gggcggcgac ttcggcatcg acgcgcacgg
     6121 cctcgcgagc cgcgaggcgc ggccgaagcg cacctgggcc agcgtcggcc tgttccgcgc
     6181 cgcgctgttc gaggacatcg tcccgggcac ggcgctcgcg ctgcggccga agctcgagtt
     6241 cgcgatcgac cgcggcgcgc tgcgcgcgaa gccgtgggac ggcgcctgga cggacgtggg
     6301 cacgcccgag cgcctcgccg cgctgcagga cgccggctga gcgcgggcgc acgaccgccc
     6361 gctcgggccg gggctcaggc cgacagcgcc ttcgtcacct cgtcgatgcg cgccatcacc
     6421 tccggcgtca gcttgtcgac cagcgccgcc gcctggaggt tgtcctgcag ctggccgacg
     6481 ctggacgcgc cggtgatcac gctgctcacg tgcgggttcc tcgcggccca ggcgatggcc
     6541 agctgcgcgg tggtgccgcc gagctcgcgc gcgatcggct cgagcttcgc gaccgcggcg
     6601 ttcttcgcct ggtcggtcag ctggtcgtgc aggaaggcca tgttctgcat cgcgccgcgg
     6661 ctgcccgtcg ggatgccctg gcggtacttg ccggtcagca ggcccgaggc gagcgggctc
     6721 cacgtcgtca ggccgaggcc gatgtcctcg tacaggcgcg cgtattcctg ctccaccttc
     6781 gcgcggtgga acaggtggta ctgcggctgc tcgacgaccg gcttgtgcag atgatggcgc
     6841 tcggcgatct cccaggccgc gcggatgtcc gacgccgtcc actcgctggt gccccagtag
     6901 agcgccttgc cctgggtgat catgtcgctc atcgcgcgca cggtctcctc gacgggcgtg
     6961 tgcggatcgc cgcggtggca gtagacgagg tcgaggtgct cgagcccgaa gcgctggagc
     7021 gagccgtcga tcgcctgcat caggtacttg cggttgagcg tgttcctgcg attgacgctc
     7081 acgcccttcg ggtcgggcag gccccagaag aacttggtcg agacgacgta gttcaggcgc
     7141 ggccagccga gcttcctcag cgcctcgccc atcacctcct cgctcttgcc gccggcatag
     7201 gcctcggcgt tgtcgaagaa gttgacgccg gcgtcgaacg cggcggccat catctcgacg
     7261 gcggcggaca cgtccacctg gttggcgtag gtcacccacg agccgagcga cagctggctc
     7321 acctgcaggc cggcgcggcc cagtcggcgg tattgcatcg atgcatcctt cggagaggcg
     7381 gatccgtttt cgtcgcccgg gtaccgacat ggcgcccggg cctcgggcca caatagcacc
     7441 ggcatccaat ccctcgcccc catggtgaca acgcacgaca cccatcgcac cgccgccccg
     7501 cggctggccg tcatcggcgg cgggccggtc ggcctcgcgc tggcgctgct cgcgcgcgaa
     7561 cggctgccgg cctggtcggt cagcgtcttc gacacccgcc ccgccgacag ggacgtgagc
     7621 ggcgacccgc gcacgctcgc gctctccctg ggcagcgtgc agctgctcga gcgcctgcgc
     7681 gcctggcccg c
//
LOCUS       DASVLN010000587         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_245387, whole genome shotgun sequence.
ACCESSION   DASVLN010000587 DASVLN010000000
VERSION     DASVLN010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245387"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VF453_14060"
     CDS             complement(<1..178)
                     /locus_tag="VF453_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HEX7688833.1"
                     /translation="MPCASPAPRAVGLFGGSFDPPHLAHRALADAALAQLGLDELVWL
                     PTGDAWHKPGRALAP"
     gene            complement(187..1137)
                     /gene="hemF"
                     /locus_tag="VF453_14065"
     CDS             complement(187..1137)
                     /gene="hemF"
                     /locus_tag="VF453_14065"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX7688834.1"
                     /translation="MPSPSAEGTAAAVDVAAVRAYLLDLQERIVASLEAADGGTFVRD
                     GWTRPADGKLTGDGLSRLIEDGALVERGGCNFSHVRGERLPPSATAARPQLAGAAFEA
                     VGVSLVIHPRNPYVPTVHMNVRLFCAFPQDGAPVFWFGGGMDLTPYNGFEDDARHFHR
                     TCAEALAPHGEALYPRFKAWCDEYFFLKHRDEPRGIGGIFFDDVDELGFEGGFAMLRA
                     VGDAFLAAYLPIVERRRALPYGERERDFQAYRRGRYVEFNLVWDRGTLFGLQSGGRTE
                     AILMSMPPLAAWRYRWSPEDGTPEARLYADFLRPRDWIAS"
     gene            complement(1118..2419)
                     /gene="purD"
                     /locus_tag="VF453_14070"
     CDS             complement(1118..2419)
                     /gene="purD"
                     /locus_tag="VF453_14070"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX7688835.1"
                     /translation="MKVLVIGSGGREHALAWKLAQSERVQRVFVAPGNGGTALDKRLV
                     NVDITDPRQLADFAVAEKVTLTVVGPEGPLAAGVVDVFRARGLRIFGPTKAAAQLESS
                     KAFSKNFMKRHGIPTAAYETFTDAAAAHAFVEKMGAPVVVKADGLAAGKGVVVATSLD
                     EAHKAVDWMLAPEGGLAVQHVADASGHAVPRVVIEEFLAGEEASFIVMVDGKNVVALA
                     SSQDHKRLADGDTGPNTGGMGAYSPAPVVTPNVHARVMHEIIVPTVEGMARDGIEFTG
                     FLYAGLMIDDKGHPKTLEFNTRMGDPETQPILMRLKSDLAEVLMHATDGTLDQVELQW
                     DRRVALGVVMAAGGYPESPRRGDTVTGIPAEADDAVVFHAGTTLDGDRLVTSGGRVLC
                     VTALGDSTRGAQQRAYEALRHIHFDGQQHRTDIGHRAIAKR"
     gene            complement(2553..3272)
                     /locus_tag="VF453_14075"
     CDS             complement(2553..3272)
                     /locus_tag="VF453_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7688836.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGKAWTRVIREIMVAARTGGGDPS
                     ANPRLRLAIEKAKAVNLPADTVKRNIDKATGNLEGVNYEEIRYEGYGIGGAAVIVDTM
                     TDNRVRTVADVRHAFSKYGGNMGTDGSVSFQFKHVGQFIFAPGAAEDRIMEVALEAGA
                     EDVVTGEDGAIEVLSAPGDFEAVKAALEAAGLAPDVAEVTMRAENTVDLAGDDSARMQ
                     KLLDALEDLDDVQEVYHNAVL"
     gene            3524..4162
                     /locus_tag="VF453_14080"
     CDS             3524..4162
                     /locus_tag="VF453_14080"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7688837.1"
                     /translation="MTLRLGDTAPDFEQESSEGTIHFHQWLGDSWGVLYSHPADFTPV
                     CTTELGMTAKLADEFRKRNVKVIALSVDPVEQHRKWIEDINDTQKTQVNFPILADADK
                     KVSNLYDMLHPNASATATVRSVFVIDPNKKIRLTLTYPASTGRNFDEILRVIDSLQLT
                     DSHSVATPVNWKDGDPVVIVPSLQDPEVLKAKFPKGWDAVRPYLRLTPQPNK"
     gene            4399..4788
                     /locus_tag="VF453_14085"
     CDS             4399..4788
                     /locus_tag="VF453_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7688838.1"
                     /translation="MDPTTPASPAPLLVACLCAQWCRTCEAYRETFAASRDAFARAHP
                     GELRFAWVDVEDESELVGDLDIEDFPTVLIARGAHVLFFGTVMPHAQTLDRLVRSALE
                     GAPALAPESLAPDVRALPGRLADRAPL"
BASE COUNT          663 a         1755 c         1686 g          699 t
ORIGIN      
        1 ccggcgcgag cgcgcggccg ggcttgtgcc acgcgtcgcc ggtgggcagc cagacgagct
       61 cgtccaggcc gagctgcgcg agggcggcgt cggcgagggc ccggtgcgcg aggtgcggcg
      121 gatcgaagct gccgccgaag agccccacgg cgcgtggcgc cggcgacgcg caaggcaggg
      181 acgcgctcag gatgcgatcc agtcgcgcgg acgcaggaag tcggcgtaca gccgcgcctc
      241 gggcgtgccg tcctcgggcg accaccggta gcgccaggcg gccagcggcg gcatcgacat
      301 caggatcgcc tccgtgcgcc cgcccgactg cagcccgaac agcgtgccgc ggtcccacac
      361 caggttgaac tcgacgtagc ggccgcggcg gtaggcctgg aaatcgcgct cgcgctcgcc
      421 gtagggcagc gcgcggcgac gctcgacgat cggcaggtag gccgcgagga aggcgtcgcc
      481 caccgcgcgc agcatcgcga agccgccttc gaagccgagc tcgtcgacgt cgtcgaagaa
      541 gatgccgccg atgccgcgcg gctcgtcgcg gtgcttcagg aagaagtact cgtcgcacca
      601 ggccttgaag cgcgggtaca gcgcctcgcc gtggggcgcg agcgcctcgg cgcaggtgcg
      661 gtggaagtgg cgggcgtcgt cctcgaagcc gttgtacggc gtgagatcca tgccgccgcc
      721 gaaccagaac accggcgcgc cgtcctgcgg gaaggcgcag aacaggcgca cgttcatgtg
      781 cacggtcggc acgtacggat tgcgcggatg gatcaccagc gagacgccga ccgcctcgaa
      841 ggccgcgccg gcaagctgcg gccgcgccgc ggtcgccgag ggcggcagcc tctcgccgcg
      901 cacgtggctg aagttgcagc cgccgcgctc gaccagcgcg ccgtcctcga tgagccgcga
      961 caggccgtcg cccgtcagct tgccgtcggc gggacgggtc cagccgtcgc gcacgaacgt
     1021 gccgccgtcg gccgcctcca ggctcgcgac gatgcgctcc tggagatcga ggaggtaggc
     1081 gcggacggcc gcgacgtcga ccgccgccgc cgtaccctca gcgcttggcg atggcacgat
     1141 ggccgatgtc cgtgcggtgc tgctgcccat cgaaatggat gtggcgcagc gcctcgtacg
     1201 cgcgctgctg cgcgccgcgg gtggagtcgc ccagcgcggt cacgcacagg acgcggccgc
     1261 cgctggtgac gaggcggtcg ccgtccagcg tcgttcccgc gtggaagacg accgcgtcgt
     1321 cggcctccgc cgggatgccc gtgaccgtat cgcccctgcg cggcgactcg ggatagccgc
     1381 ccgccgccat cacgacgccc agcgcgacgc ggcggtccca ctgaagctcg acctggtcca
     1441 gcgtgccgtc cgtcgcgtgc atcagcacct cggcgaggtc gctcttcagg cgcatcagga
     1501 tgggctgcgt ctcggggtcg cccatgcggg tgttgaactc cagcgtcttc gggtggccct
     1561 tgtcgtcgat catgaggccg gcgtagagga agccggtgaa ctcgatgccg tcgcgcgcca
     1621 tgccctcgac ggtcgggacg atgatctcgt gcatcacgcg cgcgtgcacg ttgggcgtga
     1681 ccaccggcgc cggcgagtac gcgcccatgc ccccggtgtt cgggccggtg tcgccgtcgg
     1741 ccaggcgctt gtggtcctgg ctggaggcga gcgcgacgac gttcttgccg tcgaccatca
     1801 cgatgaagct ggcctcctcg ccggcgagga attcctcgat gacgacgcgc ggcaccgcgt
     1861 ggccggaggc gtcggcgacg tgctgcacgg ccaggccgcc ctcgggcgcg agcatccagt
     1921 cgacggcctt gtgcgcctcg tccaggctcg tcgcgacgac cacgcccttg ccggccgcca
     1981 ggccgtcggc cttcacgacg acgggcgcgc ccatcttctc gacgaacgcg tgcgcggccg
     2041 ccgcgtcggt gaaggtctcg taggcggcgg tcgggatgcc gtggcgcttc atgaagttct
     2101 tcgagaacgc cttggagctc tcgagctgcg ccgccgcctt cgtcggcccg aagatgcgca
     2161 ggccgcgcgc gcggaacacg tccacgacgc cggcggccag cgggccctcc gggccgacga
     2221 ccgtcagcgt caccttctcc gcgacggcga agtcggcgag ctgccggggg tcggtgatgt
     2281 cgacgttgac gagccgcttg tccagcgccg tcccgccgtt gccgggcgcg acgaagacgc
     2341 gctgcacgcg ctcgctctgc gcgagcttcc aggcaagcgc gtgctcgcgc ccgccgctgc
     2401 cgatcacaag aactttcatg tcgatgtctc aactcgtcga ttgcgaaggg cgcgaagcgg
     2461 cggcacgagc caggcgacag ccgcggaacc ggctccgcag ggccgctggg cgtgccccgt
     2521 cggggggagg cgccgaaggc gcttcggggg ggtcatagga ccgcgttgtg atagacctcc
     2581 tggacgtcgt ccaggtcctc gagcgcgtcg agcagcttct gcatgcgcgc gctgtcatcg
     2641 ccggccaggt cgacggtgtt ctcggcgcgc atcgtcacct cggcgacgtc gggcgccagg
     2701 cccgcggcct ccagcgccgc cttgacggcc tcgaagtcgc ccggcgcgct cagcacctcg
     2761 atcgcgccgt cctcgccggt caccacgtcc tcggcgccgg cctccagcgc gacttccatg
     2821 atcctgtcct cggccgcgcc cggcgcgaag atgaactgcc cgacgtgctt gaactggaac
     2881 gacaccgagc cgtcggtgcc catgttgccg ccgtacttgg agaacgcgtg gcgcacgtcc
     2941 gccacggtgc gcacgcggtt gtcggtcatc gtgtcgacga tcacggcggc gccgccgatg
     3001 ccgtagccct cgtagcggat ctcctcgtag ttcacgccct cgaggttgcc ggtggccttg
     3061 tcgatgttgc gcttgaccgt gtcggccggc aggttgaccg ccttggcctt ctcgatggcc
     3121 agccgcaggc ggggattggc cgacgggtca ccgccgccgg tgcgcgcggc caccatgatc
     3181 tcgcggatca cccgcgtcca ggccttgccg cgcttctcgt cctgtcgacc cttgcgatgc
     3241 tggatgttgg cccatttcga gtgcccggcc atatgaggag ctcctggatg cgcgcgccgc
     3301 gggcatgccg tcacggccgc gggctgggga atttggggaa acgcgcattt tagccggtgc
     3361 ccgggcccgc gcggccgccc gccgggacgc gggcgcgggc gggaccgtcg ttgtcgcgga
     3421 aatggtcatg cgggaggctt cgttgcccgg ccgtccgcct gtggctagac tgccccgctc
     3481 gggcgcccga cgcccgcatc cgacccatcc acggagaaca gccatgacac tgcgcctcgg
     3541 cgacaccgcc cccgacttcg agcaggaatc cagcgaaggc accatccact tccaccagtg
     3601 gctcggcgac agctggggcg tcctctactc gcacccggcc gacttcaccc ccgtgtgcac
     3661 caccgaactg ggcatgacgg ccaagctggc cgacgagttc cgcaagcgca acgtgaaggt
     3721 catcgcactg tcggtcgatc cggtggaaca gcaccgcaag tggatcgagg acatcaacga
     3781 cacccagaag acccaggtca acttcccgat cctggccgat gccgacaaga aggtgtccaa
     3841 cctctacgac atgctgcacc cgaacgccag cgccacggcg accgtgcgct cggtcttcgt
     3901 catcgacccg aacaagaaga tccgcctcac gctgacctac cccgccagca ccggccgcaa
     3961 cttcgacgag atcctgcgtg tcatcgactc gctgcagctg accgactcgc acagcgtcgc
     4021 cacgccggtg aactggaagg acggcgaccc ggtcgtcatc gtgccctcgc tgcaggatcc
     4081 cgaggtgctc aaggccaagt tcccgaaggg ctgggacgcg gtgcgcccct acctgcgcct
     4141 gacgccgcag cccaacaagt gagccccccg ccggccgacg aagcacatgc cgtttgcttc
     4201 gttgcgccgc cccggggccg tcgcgacact ggcctcgcgc tctccgcaac gggggtgtga
     4261 cgggacgccg gggtttgacg ggcctcggcg gggtggaggg agcggggcag gcgcctcgct
     4321 cccttttccc ttgcgcgcgg ccctcgcgct tcaccgccgg ccggcggccg gcgaggtttg
     4381 caggcacact gccgccccat ggatccgacc accccggctt cgcccgcccc cctgctcgtc
     4441 gcctgcctgt gcgcgcagtg gtgccgcacc tgcgaggcct accgcgagac cttcgccgcc
     4501 agccgcgacg ccttcgcgcg cgcgcatccg ggcgagctgc gcttcgcgtg ggtcgacgtc
     4561 gaggacgagt ccgaactcgt cggcgacctc gacatcgagg atttcccgac cgtcctgatc
     4621 gcccgcggcg cccacgtgct gttcttcggc accgtgatgc cgcacgcgca gacgctcgac
     4681 cgcctggtgc gcagtgcgct cgagggcgcg ccggccctgg cgccggaatc gctcgcaccg
     4741 gacgtgcgcg cgctgccggg gcggctggcg gatcgcgcgc cgctgtgagc tcccggcctc
     4801 agg
//
LOCUS       DASVLN010000588         5887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_353190, whole genome shotgun sequence.
ACCESSION   DASVLN010000588 DASVLN010000000
VERSION     DASVLN010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353190"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..394)
                     /locus_tag="VF453_14090"
     CDS             complement(35..394)
                     /locus_tag="VF453_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7688839.1"
                     /translation="MALEHAQPAQPWDVRPLGASIAGQSSRALFKSRQLEVIRLVLPA
                     GRSLPEHRVAGELTLHCLEGDLVVQAQGATHRLGAGQLLFLPGGVPHSVTAQVDSSAL
                     LTLVLAPGEEGGQGAAA"
     gene            complement(606..959)
                     /gene="rplS"
                     /locus_tag="VF453_14095"
     CDS             complement(606..959)
                     /gene="rplS"
                     /locus_tag="VF453_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX7688840.1"
                     /translation="MDLIATLEKEEIARLGKNIPTFAPGDTVIVNVNVVEGTRKRVQA
                     YEGVVISRRNRGLNSNFIVRKISSGEGVERTFQLYSPLIASIEVKRRGDVRRAKLYYL
                     RERSGKSARIKEKLA"
     gene            complement(1098..1871)
                     /gene="trmD"
                     /locus_tag="VF453_14100"
     CDS             complement(1098..1871)
                     /gene="trmD"
                     /locus_tag="VF453_14100"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7688841.1"
                     /translation="MRFDVVTLFPDLFAPFLAQGVTRRAFESRQVDVRLWNLRDHADG
                     NYRRVDDRPFGGGPGMVMLAEPLERALDAVARARADAGAPAGPVVLFTPTGRRIDQAL
                     VREWAAGPGAVLVCGRYEGVDQRVVDRRVDVELSLGDFVLSGGELPAMTLLDAIARLQ
                     PGVLGDEQSHLQDSFEHGLLDCPHYSRPEVLATSAGEVPVPAVLLSGHHADIARWRRE
                     QSLRLTAERRPDLIAAARAAGRLTKADEKFLANLAPGTL"
     gene            complement(1888..2601)
                     /gene="rimM"
                     /locus_tag="VF453_14105"
     CDS             complement(1888..2601)
                     /gene="rimM"
                     /locus_tag="VF453_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345719.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX7688842.1"
                     /translation="MTADERAEDLSWPEDAVEVGRILGAWGIKGGIKVAPFAADPQAL
                     FSAKRWFLKPAESAKPPSARTAAATAAKPAPAAASSRKTLAQRAALAAPLPVALHVKG
                     VRDQGDAIVATAPEIPDRDAAEAMKGVRIFVSRASFPAPDEDEFYWVDLIGLSVVNRD
                     GAALGEVVGLIDTGPHCVLRVQPPAEASAAAAPDAAKSGAPASDAAAEERLIPFVEAY
                     VDQVDMPGRRIVVDWGLDF"
     gene            complement(2632..2901)
                     /gene="rpsP"
                     /locus_tag="VF453_14110"
     CDS             complement(2632..2901)
                     /gene="rpsP"
                     /locus_tag="VF453_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX7688843.1"
                     /translation="MVVIRLARGGSKKRPFYNIVVADSRERRDGRYLERVGFYNPVAH
                     VNEEGLRVVFDRVTYWKGVGAQASPTVERLLDQAAKKVAAAPAAA"
     gene            3216..3782
                     /locus_tag="VF453_14115"
     CDS             3216..3782
                     /locus_tag="VF453_14115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7688844.1"
                     /translation="MQEQFASSSPLVLRAGTDADVAACAAIYREAVRNGTGTFELDEP
                     DDAEMARRREAILAQGLPWLVAERDGGVLGYAYANQFRPRRAYRFCVEDSIYLDPAAR
                     GQGVGRLLLAELLARCEALGIRQVLAVIGDSANAGSIAVHRACGFVDAGKFEAAGWKF
                     DRWLDVVLMQRRLGAGAATAPVERGHVA"
     gene            3779..4279
                     /locus_tag="VF453_14120"
     CDS             3779..4279
                     /locus_tag="VF453_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688845.1"
                     /translation="MNAASPELAARVRDPAEVPSKTLATWIAVAGGAIGLHHFYLHGW
                     RTRLGWLYPLPTLIGLFGVLRMRGQGVDDRLSWLLIPWLGLTISAGMLCAIVIGLTSD
                     ARWARRWAVEPPQPAPPPDEDDESPPGLVRTGWAPVLGVILALMIGGGVLMGTIAFTV
                     QKIFEW"
     gene            complement(4251..5213)
                     /locus_tag="VF453_14125"
     CDS             complement(4251..5213)
                     /locus_tag="VF453_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688846.1"
                     /translation="MSRQDVNRAAEMEVFVHAVEAGGFSAAARALGMTPSAVSKLVAR
                     LEARLGTRLLQRSTRALQLTAEGCVFYERSRRVLADIDEAERLAASAAAPRGRVSVNT
                     NVPFGVHVLLPLLPRFLERHPEITLDVVLTDEVVDLLEKRTDVAIRGGPMKDSRLVAR
                     KLGHTRWVVAGSPAYLARAGVPRRPEDLAHHNRIGFNYARALEGWPFVVDGAPVNVPV
                     DGNIRLSDGDALRRTALAGGGLVRLAWFMLREDVLARRLQVVLEDFNPGDTEDFHAVF
                     IGAGGHLPTRVRALLDFLADEVRLDQGPTKRELARLTTRRSSAP"
     gene            5339..>5887
                     /locus_tag="VF453_14130"
     CDS             5339..>5887
                     /locus_tag="VF453_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7688847.1"
                     /translation="MPLALYALTVGAFGIGVTEFVIMGLLPQVAAGFGVSLEAAGALI
                     SGYALGVVVGAPVLTVATRAWPRKTVLLALMAVFTVGNALCALAPSYGLLMVARVLTS
                     FAHGSFFGVGSVVAAGLVAPNRRASAIAAMFTGLTLANVLGVPFGTWLGQALGWRATF
                     EAVAVVGALALAVIALAVPRDTA"
BASE COUNT          785 a         2089 c         2186 g          827 t
ORIGIN      
        1 catcgaggac gcgtgccccg gcggcggccg tccgtcaggc cgcggcgccc tggccgccct
       61 cctcgcccgg ggccagcacg agcgtcagca gcgcgctgga atccacctgg gcggtcaccg
      121 aatgcggcac gccgccgggc aggaacagca gttggccggc gcccagccgg tgcgtcgcgc
      181 cctgcgcctg gacgacgaga tcgccctcga ggcagtgcag cgtcaactcg cccgcgaccc
      241 ggtgctcggg caacgagcgt ccggccggca gcacgagccg gatgacctcg agctgccgcg
      301 acttgaacag cgcccgcgac gactgcccgg cgatcgaggc gcccagcggg cggacgtccc
      361 acggttgcgc gggctgcgcg tgttcgaggg ccatggcgtg ctccttgcgt ggatggctcg
      421 cgacgactat aggcctcgcc gggacgaccg cgccggcagg cgagtcttcg gcggcggaag
      481 gtgcgcggca cgcgggcctc gggcgcagat ccgcgggcac cgcggcactg gccggcccgc
      541 aatgcagaaa gcccggacga gccgggcttt cggggcgttc gacgcgtccc gcgggacgcg
      601 gccgatcagg ccagcttttc cttgatgcgc gccgacttgc ccgagcgctc gcgcaggtag
      661 tacagcttgg cgcggcggac gtcgccgcgg cgcttgacct cgatcgaggc gatcagcggg
      721 ctgtacagtt ggaacgtgcg ctccacgcct tcgccgctgg agatcttgcg cacgatgaag
      781 ttcgagttca ggccgcggtt gcgacgcgag atcacgacgc cttcgtaggc ctggacgcgc
      841 ttgcgcgtgc cttcgacgac gttgacgttg acgatcaccg tgtcgccggg ggcgaacgtc
      901 gggatgttct tgccgagacg ggcaatctct tccttctcga gggtagcgat cagatccatg
      961 ggatgctttc ggttcgtcgg ttcttgccgg cgcactattg ctcggagtgc agcactggag
     1021 tccgtccagg cgccaggacg gcacggagtc cgtcgctgat cggctgcttc tgcccggcag
     1081 aggaacagag cccgcgatta tagcgtgccg ggcgccaggt tggcgagaaa cttctcgtcc
     1141 gccttggtca gccgacccgc cgctcgggcg gcggcgatca ggtcggggcg gcgctcggcg
     1201 gtcagcctca gggactgctc gcggcgccag cgcgcgatgt cggcgtggtg gccggacagc
     1261 agcaccgccg gcaccggcac ctcgcccgcg gacgtggcga gcacctcggg ccggctgtag
     1321 tgcgggcagt cgagcaggcc gtgctcgaag ctgtcctgca agtgcgactg ctcgtcgccc
     1381 agcacgcccg gctgcaggcg ggcgatcgcg tcgagcagcg tcatcgccgg cagctcgccg
     1441 ccggacagca cgaagtcgcc caggctcagc tcgacgtcga cgcggcggtc gacgacgcgc
     1501 tggtcgacgc cctcgtagcg cccgcacacc agcaccgcac cggggccggc agcccactcg
     1561 cgcacgagcg cctggtcgat gcgccggccg gtgggcgtga acagcacgac cggccccgcg
     1621 ggcgcgcccg cgtcggcgcg tgcgcgcgcg acggcgtcga gcgcgcgttc cagcggctcg
     1681 gccagcatca ccatcccggg gccgccgccg aacggacggt cgtcgacgcg acggtagttg
     1741 ccgtccgcgt gatcgcgcag gttccacagc cggacgtcga cctggcgcga ctcgaacgcg
     1801 cgccgcgtca cgccctgcgc gaggaagggt gcgaacaggt cgggaaacag cgtgacgacg
     1861 tcgaagcgca tgcggcgccc caggcgatca gaaatcgagg ccccagtcga cgacgatgcg
     1921 ccggccgggc atgtcgacct ggtcgacgta ggcctcgacg aagggaatga ggcgctcctc
     1981 ggcggcggcg tcgctcgcgg gggcgcccga cttcgcggcg tccggcgcgg cggcggcgga
     2041 cgcctcggcg ggcggttgca cgcgcagcac gcagtgcggg ccggtgtcga tcaggccgac
     2101 gacctcgccg agcgccgcgc cgtcgcggtt gacgacggac aggccgatca ggtcgaccca
     2161 gtagaactcg tcctcgtcgg gcgccgggaa gctggcgcgc gagacgaaga tgcgcacgcc
     2221 cttcatcgcc tcggcggcgt cgcggtcggg gatctcgggc gcggtggcga cgatcgcgtc
     2281 gccctggtcg cgcacgccct tgacgtgcag cgcgacgggc agcggcgcgg cgagcgcggc
     2341 ccgttgcgcg agtgtcttgc gggaggaagc ggccgcggga gcaggcttgg ccgcggtggc
     2401 ggcggcggtc ctggcggacg gcggcttcgc gctctcggcc ggcttcagga accagcgctt
     2461 ggcggagaac agggcctgtg gatccgcggc gaagggcgcc accttgatgc cgcccttgat
     2521 gccccaggcg ccgaggatgc ggccgacctc gaccgcgtcc tcgggccagg acaggtcttc
     2581 cgcgcgctcg tcagccgtca tcgaagcaag cgcgcggaag cgatcggccg atcaggccgc
     2641 ggccggagcg gcggcgacct tcttggcggc ctggtccagc aggcgctcga ccgtcgggga
     2701 cgcctgggcg ccgacgccct tccagtaggt cacgcgatcg aagacgacgc gcaggccttc
     2761 ctcgttgaca tgcgccaccg ggttgtagaa acccacgcgc tcgaggtagc ggccgtcgcg
     2821 gcgctcgcgc gagtcggcga cgacgatgtt gtagaacgga cgcttcttgg agccgccgcg
     2881 ggcaagtcgg atcacgacca tgatgttcca atcgatttgt cgggtgtgct cgccgggcca
     2941 gggagacacg caaggccggg acgaactgca agtcccgcgg ccgaagcgcg cgggacgact
     3001 cctccggcac cgttagatca cgccgacgtt cctgcaacga cggatgcttt ggacgccagt
     3061 ccgcgcgaag ggtcagttcg caagctccag gcaagaggtc ggccgacgcc gaaaaccgcg
     3121 cattataaac tgaacgtccg ccatgtccag gatggcagcg tccggccacg ggccgggctt
     3181 gtcgagcctc caacatatcg gacaaactct ccaccatgca agaacagttc gcctcctcct
     3241 cgccgctggt gctgcgcgcc ggtaccgatg ccgacgtcgc cgcctgcgcc gcgatctacc
     3301 gcgaggccgt gcgcaacggc acgggcacct tcgagctcga cgaacccgac gacgccgaga
     3361 tggcgcgccg ccgggaggcg atcctggcgc agggcctgcc ctggctggtc gccgagcgcg
     3421 acggcggcgt cctgggctac gcctacgcca accagttccg gccgcggcgc gcctatcgct
     3481 tctgcgtcga ggactcgatc tacctcgacc ccgccgcgcg cggccagggg gtcggtcggc
     3541 tgctgctggc cgagctgctc gcgcgctgcg aggcgctggg catccgccag gtgctggcgg
     3601 tgatcggcga ctcggccaac gcgggctcga tcgccgtcca ccgcgcctgc ggcttcgtgg
     3661 acgccggcaa gttcgaggcg gcgggctgga agttcgaccg ctggctggac gtcgtgctga
     3721 tgcagcgccg cctgggcgcc ggcgcggcga cggcgccggt cgaacgcggg cacgtggcgt
     3781 gaacgccgcc tcgcccgaac tcgcggcgcg cgtgcgcgat ccggcggagg tgccgtccaa
     3841 gacgctcgcg acctggatcg cggtggccgg cggggccatc ggcctgcacc acttctacct
     3901 gcacggctgg cgcacgcggc tcggctggct ctatccgctg ccgacgctga tcggcctgtt
     3961 cggcgtgctg cgcatgcgtg ggcagggcgt cgacgaccgg ctctcgtggc tgctgatccc
     4021 gtggctcggg ctgacgatct ccgccggcat gctctgcgcg atcgtgatcg gcctgacgtc
     4081 ggacgcgcgg tgggcgcgtc gctgggccgt cgagcccccg cagcccgcgc cgccgcccga
     4141 cgaggacgac gagtcgccgc ccggcctcgt gcgcacgggc tgggcgccgg tcctgggcgt
     4201 gatcctcgcg ctcatgatcg gcggcggcgt gctgatgggc acgatcgcgt tcacggtgca
     4261 gaagatcttc gagtggtgag ccgcgcgagc tcgcgcttcg tcggcccctg gtcgagcctc
     4321 acctcgtcgg cgaggaagtc cagcagcgcc cgcacccgcg tcggcaggtg cccgccggcg
     4381 ccgatgaaga cggcgtggaa gtcctcggtg tcgcccgggt tgaagtcctc gagcaccacc
     4441 tgcagccggc gggcgagcac gtcctcgcgc agcatgaacc acgcgaggcg cacgaggccg
     4501 ccgccggcca gcgcggtgcg gcgcagcgcg tcgccgtcgc tcaggcggat gttgccgtcg
     4561 accggcacgt tgaccggcgc gccgtcgacg acgaagggcc agccctccag cgcccgcgcg
     4621 tagttgaagc cgatgcggtt gtggtgcgcg agatcctcgg gccggcgcgg cacgcccgcg
     4681 cgggcgaggt aggccggcga gcccgcgacg acccagcgcg tgtgtcccag cttgcgcgcg
     4741 accaggcgcg agtccttcat cggcccgccg cggatcgcga cgtcggtgcg cttctcgagc
     4801 aggtcgacca cctcgtcggt gagcacgacg tccagcgtga tctcgggatg gcgctccagg
     4861 aagcgcggca gcagcggcag cagcacgtgc acgccgaagg gcacgttggt gttgaccgag
     4921 acccggcccc gcggcgccgc ggccgaggcg gcgagacgct ccgcttcgtc gatgtcggcg
     4981 agcacgcggc ggctgcgctc gtagaagacg cagccctccg cggtgagctg caacgcgcgc
     5041 gtggagcgct gcagcagccg cgtgcccagg cgcgcctcga gccgcgcgac cagcttgctg
     5101 accgccgacg gcgtcatccc gagggcgcgc gccgccgccg agaagccgcc cgcctcgacg
     5161 gcatgcacga acacctccat ctcggcggcg cggttgacgt cctggcggct catcgcgccg
     5221 tcctcgacct gtgacttgga ttcatggatg gatgtcccgc gggcagtcta gtcgcaggcg
     5281 ggcggcgccg ccagcatcgg ggcatcgcca tcgaaccctc gaagacaagg aaacggacat
     5341 gccgctcgcc ctctacgcgt tgacggtcgg tgccttcggc atcggcgtca cggaattcgt
     5401 catcatggga ctgctgcccc aggtcgccgc cggcttcggc gtctcgctgg aggcggccgg
     5461 cgcgctgatc tcgggctatg cgctgggcgt cgtggtcggc gcgccggtgc tgaccgtcgc
     5521 cacgcgcgcg tggccgcgca agacggtgct gctcgcattg atggccgtct tcacggtcgg
     5581 caacgcgctg tgcgcgctcg cgccgagcta cgggctgctg atggtggcgc gcgtgctgac
     5641 ctcgttcgcg cacggcagct tcttcggcgt cggctcggtc gtcgcggccg ggctcgtggc
     5701 gccgaaccgg cgcgcctcgg cgatcgcggc gatgttcacc ggcctcacgc tcgccaacgt
     5761 gctgggcgtg cccttcggca cctggctcgg ccaggcgctg ggctggcgcg cgaccttcga
     5821 ggccgtcgcc gtcgtcgggg cgctcgcgct ggcggtgatc gcgctggccg tgccgcgcga
     5881 caccgcg
//
LOCUS       DASVLN010000589         1953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_245542, whole genome shotgun sequence.
ACCESSION   DASVLN010000589 DASVLN010000000
VERSION     DASVLN010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245542"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VF453_14135"
     CDS             <1..1011
                     /locus_tag="VF453_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX7688848.1"
                     /translation="DAARQGRVDLNDRTNLARTLSSMLSVGTGASIGREGAMVQLSAW
                     ASARLSRLVPIAPERRSALLVCGIAAGIASAYRAPVAGVVFVLELALGFFARHTVAPV
                     LIASGTASALVYVLVDSHPLYAVAPVTMLPSSLGFALVAGVLFGGLGWGFLRLVERSR
                     ELFARVRSPALRLGLGGLLVGVLSAFVPEVWGNGYSVIGEALAGTNTWQWLALVLITK
                     VVATSWSSGSGTVGGMFTPSLFVGAAAGGVLAQVAALWLPTAWIGDPHSLVVIGMAAV
                     LAAVTHAPLMAITMVLEMTNEFQLTVPVMLACGIAYAMSTRFGTKPLYGNPIEAARKP
                     SD"
     gene            1223..>1953
                     /locus_tag="VF453_14140"
     CDS             1223..>1953
                     /locus_tag="VF453_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HEX7688849.1"
                     /translation="MTQKNLPRAAIAAALSSLALAATPLAHAQISIGVGINIAPPPLP
                     YYVQPPIPGDGYIWTPGYWSWDPATGDYVWVPGTWVLPPAVGLLWTPGYWAFYGGGYR
                     WHRGYWGPHVGYYGGINYGYGYGGHGYAGGHWDRGHFAYNRAVNNVPEGRVHAAYGRP
                     MAVRPMPAHDSFNGHGSHFTAPPTAAERRFEGARHGQPTPEQVEHEHRAVATPEQRMA
                     NNHGMPPIAATPRPSGFGEPGFERGR"
BASE COUNT          225 a          721 c          717 g          290 t
ORIGIN      
        1 gatgccgcgc gccagggccg cgtcgacctc aacgaccgca ccaacctcgc ccgcacgctg
       61 tcgtcgatgc tgtcggtggg caccggcgca tcgatcgggc gcgagggcgc gatggtgcag
      121 ctctcggcct gggcgagcgc ccggctgtcg cgcctcgtgc cgatcgcgcc ggagcgccgc
      181 agcgcgctgc tcgtgtgcgg catcgcggcg ggcatcgcgt cggcctaccg ggcgccggtc
      241 gccggcgtcg tcttcgtgct ggaactcgcg ctcggcttct tcgcgcgcca tacggtcgcg
      301 ccggtgctga tcgcctcggg caccgcgagc gcactcgtct acgtgctggt cgactcgcac
      361 ccgctgtacg ccgtcgcgcc cgtgaccatg ctgccgtcca gcctcggctt cgcgctcgtg
      421 gcgggcgtgc tgttcggcgg gctgggctgg ggcttcctgc gcctggtcga gcgcagccgc
      481 gagctgttcg cgcgcgtgcg ctcgccggcg ctgcggctgg gcctgggcgg actgctggtg
      541 ggcgtgctgt cggccttcgt gcccgaagtc tggggcaacg gctacagcgt gatcggcgag
      601 gcgctggccg gcacgaacac gtggcagtgg ctcgcgctgg tgctgatcac caaggtcgtc
      661 gcgacgtcgt ggagctcggg cagcggcacg gtgggcggca tgttcacgcc gtcgctgttc
      721 gtcggcgcgg cggcgggtgg cgtgctcgcg caggtggcgg cgctgtggct gcccacggca
      781 tggatcggcg acccgcattc gctggtcgtg atcggcatgg ccgcggtgct ggccgccgtc
      841 acgcatgcac cgctgatggc catcacgatg gtgctggaga tgaccaacga gttccagctc
      901 accgtgcccg tgatgctcgc ctgcgggatc gcctacgcca tgagcacgcg cttcggcacc
      961 aagccgctgt acggcaaccc gatcgaggcg gcgcgcaagc cgtcggactg agtcgcggtc
     1021 gcgctcccgt cgagcggcga agccgcatgg atgaacgacc cgcctccgcg ggtccgtcga
     1081 ttgccggtcc atggtgcagc gccgccgcgc ccgggcgcct cctgcgacgc cgccgctccc
     1141 ccgagcggtc gcgtgcgtgc gctgcctcgt ctttccggat ggcacgcgcg cgcgagacgc
     1201 gcgcactcgc gagggctgct ccatgaccca gaagaacctt ccgcgcgcgg cgatcgccgc
     1261 cgcgctctcg agcctcgcgc tggcggccac gccgctggcg catgcgcaga tctccatcgg
     1321 cgtgggcatc aacatcgcgc cgccgccgct gccctactac gtgcagccgc ccatcccggg
     1381 cgacggctac atctggacgc cgggctactg gagctgggat ccggccaccg gcgactacgt
     1441 ctgggtgccg ggcacctggg tgctgccgcc cgcggtgggc ctgctgtgga cgccgggcta
     1501 ctgggcgttc tacggcgggg gctatcgctg gcaccgcggg tactggggtc cgcacgtcgg
     1561 ctactacggc ggcatcaact acgggtacgg gtatggcggc cacggctacg ccggcgggca
     1621 ctgggatcgc gggcacttcg cctacaaccg cgccgtcaac aacgtcccgg agggccgtgt
     1681 ccacgctgcc tacggccgcc cgatggcggt gcggccgatg ccggcgcacg acagcttcaa
     1741 cggccacggc tcgcacttca ccgcgccgcc gacggccgcc gagcgccgct tcgagggcgc
     1801 ccggcacggc cagcccacgc ccgagcaggt cgagcacgag catcgcgccg tcgccacgcc
     1861 ggagcagcgc atggccaaca accacggcat gccgccgatc gccgcgacgc cgcggccgtc
     1921 gggcttcggc gagccgggct tcgagcgcgg ccg
//
LOCUS       DASVLN010000590         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_407146, whole genome shotgun sequence.
ACCESSION   DASVLN010000590 DASVLN010000000
VERSION     DASVLN010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407146"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..506)
                     /locus_tag="VF453_14145"
     CDS             complement(177..506)
                     /locus_tag="VF453_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688850.1"
                     /translation="MSRHKMHKTLAVAALALVAGASFAQGTFREDARDGELAARIEQG
                     RASGSLTPREVEHLRREQERIRHVEWRNKRDGVVTRGEREQVRLMQDRLSEDISHEKH
                     NGRADYR"
     gene            649..2112
                     /gene="tldD"
                     /locus_tag="VF453_14150"
     CDS             649..2112
                     /gene="tldD"
                     /locus_tag="VF453_14150"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEX7688851.1"
                     /translation="MITREPTLERLAIAEGLLLEPFGLAESDLAATLAAMGEHRIDDA
                     DLYFQTTRHEGWSLEEGIVKSGSFSIDQGVGVRAVSGEKTAFAYSDDISAASLMDAAR
                     TVRAIGAAGQSRRVKLQTKTKVSPSRLLYGTADPIATLDSTQKVALLERVEKMARARD
                     PRIVQVMAGLAAEHDVVLVARADGTRAADVRPLVRLSITVIAEQNGRRETGSGGGGGR
                     FGLGYFTEGMLSKYVDQAVNAALTNLESRPAPAGEMTVVLGPGWPGVLLHEAVGHGLE
                     GDFNRKGSSTFAGRIGKRVAAKGVTVLDDGTIADRRGSLNVDDEGNASQRNVLIEDGI
                     LRGYIQDSLNARLMGVKPTGNGRRESYAHTPMPRMTNTYMLAGDKPPEEIVASIRRGL
                     YATNFGGGQVDITSGKFVFSASEAYWVENGRIQYPVKGATLIGNGPDALTRISMIGND
                     LELDSGVGVCGKEGQSVPVGVGQPTLRIDGLVVGGTA"
     gene            2343..3473
                     /locus_tag="VF453_14155"
     CDS             2343..3473
                     /locus_tag="VF453_14155"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX7688852.1"
                     /translation="MTNPKHPGEARHGLSLSERTSDTDDQRIRDVTPLPPPEHLIRFF
                     PIAGTPVEGLVGDTRAAVRRILSGEDDRLLVVIGPCSIHDPAAALDYARRLRAEREKH
                     AGTLEIVMRVYFEKPRTTVGWKGLINDPYLDESYRIHEGLRIARQLLLDINRLGVPAA
                     GEFLDTISPQYIGDLISWGAIGARTTESQVHRELASGLSAPVGFKNGTDGNLKIAIDA
                     IQAAARPHHFLSVHKNGQVAIVQTGGNEDCHVILRGGRQPNYDAASVAAACRELEAAK
                     LDCKVMIDFSHANSEKKFERQVAVARDVADQVASGNGCIFGVMVESHLHGGAQKFTPG
                     KDDPATLEYGKSITDGCIGWDDSLAVLETLGQAVLARRAARG"
     gene            complement(3490..4077)
                     /locus_tag="VF453_14160"
     CDS             complement(3490..4077)
                     /locus_tag="VF453_14160"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7688853.1"
                     /translation="MSNRLSQIATRTGDDGTTGLGDGSRVPKSHLRIAAMGDVDELNS
                     HLGVLLAEPLPDDVRTLLVTVQHELFNLGGELSIPGYTLLKDDAVALLDDALARHNAT
                     LPRLAEFILPAGTRSAALAHVARAVARRAERAVVTLAAADAVNPAPRLFLNRLSDLLF
                     VLARVLNRANLDGLGGDDVYWKSEKLARNLDPDVA"
     gene            4240..5610
                     /locus_tag="VF453_14165"
     CDS             4240..5610
                     /locus_tag="VF453_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7688854.1"
                     /translation="MPPEMAHALGAHFGARYVTSMAVREQHGRDESPIDAPPPEAVVF
                     CESTADVAFVVKQAATHRVPVIPYGAGSSLEGHLLAVQGGVSVDVSRMDAVLQLNAED
                     LTVTVQAGVTRMQLNREIKDSGLFFPIDPGADATIGGMSATRASGTNAVRYGTMRENV
                     LALTVVTPSGEVIRTGTRARKSSAGYDLTRLVVGSEGTLGVITEVTLRLHPLPEAVSA
                     AICSFADVRAAVDTVIEIIQVGVPIARCELLDANAVRAVNAHDKLGLAETPMLLMEFH
                     GSDSGVAEQAATVQALAAGHGGTAFQWATTPEERTRLWTARHHAFLSGLQMRPGCRAV
                     TTDCCVPISRLADAVTQALAEADAAGMPHFLVGHVGDGNFHVAYLIDPDEPKDRATAE
                     RLNHALVQRAIAAGGTCTGEHGIGLHKQGFLVEEAGLGAVAMMRQIKRALDPDNIMNP
                     GKIFEL"
     gene            complement(5627..5773)
                     /locus_tag="VF453_14170"
     CDS             complement(5627..5773)
                     /locus_tag="VF453_14170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7688855.1"
                     /translation="MLAAAEDRARAAGCSHVELSSNERRTRAHAFYEREGFAEVRRRF
                     VKRF"
BASE COUNT          858 a         2080 c         2065 g          770 t
ORIGIN      
        1 cgcggccgag cgcacgctgc gcgtcctgct gcgcgagctg cccgtccggc aggcggccgc
       61 gctggcggcc gagctctgcg acatgccgcg caagcggctc tacgcgcagg ccctggcctg
      121 gaagggcgag ggcgaggacg acgcgggcga gtgagcgcct gccccgccca gggggctcag
      181 cgatagtccg cgcggccgtt gtgcttctcg tgcgagatgt cctcgctcag gcggtcctgc
      241 atcaggcgga cctgctcgcg ctcgccgcgc gtcacgacgc cgtcgcgctt gttgcgccac
      301 tcgacgtgac ggatgcgctc ctgctcgcgc cgcaggtgct cgacctcgcg cggcgtcagg
      361 ctgccgctcg cccgcccctg ctcgatgcgg gcggcaagct cgccgtcgcg cgcgtcctcg
      421 cggaaggtgc cctgcgcgaa gctcgcgccg gccaccaggg cgagggccgc gacggccagg
      481 gtcttgtgca tcttgtgtcg ggacatgtca gactccttct tcgttcgggc catggccatg
      541 ccgggggcgt tgcgccgccg gcggcccctg cgcccgaaat gagttaagtt ccatgagcct
      601 cgccggcact gccggcttgg tctctttttg gcaatccagg agcccgcctt gatcacccgc
      661 gaacccacgc tcgaacgcct tgccatcgcc gagggcctcc tgctcgaacc cttcggcctg
      721 gccgagtccg acctcgccgc gaccctggcc gcgatgggcg agcaccggat cgacgacgcc
      781 gacctctact tccagaccac ccgccacgag ggctggagcc tcgaggaagg catcgtcaag
      841 agcggcagct tctcgatcga ccagggcgtg ggcgtgcgcg cggtctcggg cgagaagacg
      901 gcgttcgcgt actccgacga catctcggcc gcgtcgctga tggacgccgc ccgcacggtg
      961 cgcgcgatcg gcgcggccgg gcagagccgg cgcgtgaagc tgcagacgaa gacgaaggtg
     1021 tcgccgagcc ggctgctcta cggcaccgcg gatccgatcg cgacgctcga cagcacccag
     1081 aaggtcgcgc tgctcgagcg cgtcgagaag atggcgcgtg cccgcgaccc gcgcatcgtg
     1141 caggtgatgg ccgggctcgc ggccgagcac gatgtcgtgc tggtcgcgcg cgcggacggc
     1201 acgcgcgcgg ccgacgtgcg gccgctggtg cgcctgtcca tcaccgtgat cgccgagcag
     1261 aacggccgcc gcgagaccgg ctcgggcggc ggcggcggcc gcttcggcct gggctacttc
     1321 accgagggca tgctgtcgaa gtacgtcgac caggccgtca acgcggcgct caccaacctc
     1381 gagtcgcgcc ccgcgcccgc gggcgagatg accgtggtgc tcggccccgg ctggcctggc
     1441 gtgctgctgc acgaggcggt cggccacggc ctggaggggg acttcaaccg caagggctcg
     1501 agcaccttcg ccggccgcat cggcaagcgc gtcgcggcca agggcgtcac cgtgctggac
     1561 gacggcacga tcgcggaccg ccgcggctcg ctcaacgtcg acgacgaggg caacgcgagc
     1621 cagcgcaacg tgctgatcga ggacggcatc ctgcgcggct acatccagga ctcgctcaac
     1681 gcgcgcctga tgggcgtcaa gcccaccggc aacggccgcc gggagagcta cgcccacacg
     1741 ccgatgccgc gcatgaccaa cacctacatg ctggccggcg acaagccgcc cgaggagatc
     1801 gtcgcctcga tccggcgcgg gctgtacgcg accaacttcg gcggcggcca ggtcgacatc
     1861 acgagcggca agttcgtgtt ctcggccagc gaggcctatt gggtcgagaa cggccgcatc
     1921 cagtatccgg tcaagggcgc gacgctgatc ggcaacggac ccgacgcgct gacccgcatc
     1981 tcgatgatcg gcaacgacct cgagctcgac tccggcgtcg gcgtctgcgg caaggagggc
     2041 cagagcgtgc ccgtcggcgt cggccagccg acgctgcgca tcgacgggct ggtcgtcggc
     2101 ggcacggcat gacgaagcga cgcttgaccc ggcgcgaagc gcgcgccggg acgagccgtg
     2161 ctacattgcg gcgtccatga acacgtcccg cttcttcttc gcgtactttt ggttctcgcc
     2221 ccgtcaggtg gctggagagg caatcgcacg ctgaagcgaa cgagaccccc gaaagaccgc
     2281 cggccaaccc cggcggtttt ttttcgccgg acgaggcctc gaaactccct ggagccgaac
     2341 cgatgacgaa tccgaagcac cccggcgaag cccgccacgg cctgtcgctc agcgagcgca
     2401 ccagcgacac cgacgaccag cgcatccgcg acgtgacgcc gctgccgccg cccgagcacc
     2461 tgatccgctt cttcccgatc gcgggcacgc ccgtcgaggg gctggtcggc gacacccgcg
     2521 ccgccgtgcg ccggatcctc tcgggcgagg acgaccgcct gctcgtcgtc atcgggccct
     2581 gctcgatcca cgatcccgcc gccgcgctgg actacgcgcg ccgcctgagg gccgagcgcg
     2641 agaagcacgc cggcacgctc gagatcgtga tgcgcgtgta cttcgagaag ccccgcacca
     2701 ccgtcggctg gaaaggcctg atcaatgacc cgtacctcga cgagagctac cgcatccacg
     2761 aaggcctgcg gatcgcgcgc cagctgctgc tcgacatcaa ccggctcggc gtgccggcgg
     2821 ccggcgagtt cctcgacacg atctcgccgc agtacatcgg cgacctgatc agctgggggg
     2881 cgatcggcgc ccgcacgacc gagagccagg tgcaccgcga gctcgcgtca ggcctgtcgg
     2941 cgccggtggg cttcaagaac ggcaccgacg gcaacctgaa gatcgccatc gacgccatcc
     3001 aggccgcggc ccggccgcac catttcctct cggtgcacaa gaacggccag gtcgcgatcg
     3061 tgcagaccgg cggcaacgag gactgccacg tcatcctgcg cggcggcagg cagcccaact
     3121 acgacgccgc cagcgtcgcc gcggcctgcc gggagctcga ggccgccaag ctggactgca
     3181 aggtgatgat cgacttcagc cacgccaaca gcgagaagaa gttcgagcgc caggtcgcgg
     3241 tcgcgcgcga cgtcgcagac caggtcgcct cgggcaacgg ctgcatcttc ggcgtgatgg
     3301 tcgagagcca cctccacggc ggcgcgcaga agttcacgcc gggcaaggac gatccggcga
     3361 cgctcgaata cggcaagagc atcaccgacg gctgcatcgg ctgggacgac tcgctggccg
     3421 tgctggagac gctcgggcag gccgtgctgg cgcggcgcgc ggcccgcggt tgagggcgcg
     3481 cgtgccgcct caggcgacgt cggggtcgag gttgcgcgcg agcttctcgc tcttccaata
     3541 gacgtcgtcg ccgcccaggc cgtcgaggtt ggcgcggttg aggacgcgcg cgaggacgaa
     3601 cagcaggtcc gacagccggt tcaggaacag ccgcggcgcc gggttgaccg cgtcggccgc
     3661 ggccagcgtc acgaccgcgc gctcggcgcg ccgcgccacc gcgcgcgcga cgtgcgcgag
     3721 cgccgcgctg cgcgtgcccg ccggcaggat gaactcggcc aggcgaggca gcgtcgcgtt
     3781 gtggcgcgcc aacgcgtcgt ccagcagcgc gaccgcgtcg tccttgagca gcgtgtagcc
     3841 cgggatcgag agctcgccgc cgaggttgaa cagctcgtgc tgcacggtca cgagcagcgt
     3901 gcgcacgtca tccggcaggg gctcggcgag cagcacgccc aggtgcgagt tcagctcgtc
     3961 gacgtcgccc atcgcggcga tgcgcaggtg gctcttgggc acgcgcgagc cgtcgccgag
     4021 accggtcgtg ccgtcgtcgc cggtgcgggt ggcgatctgg gagaggcgat tggacatggc
     4081 gtgcagtgta ggccacgggt gcggcatgcg aacccgccgc tgcgaggcga cggccgcggg
     4141 cgtagactgg cccgcggcgt ccgcccgcgc gacgggcgcg ctgcaggagc cgccatgaac
     4201 accgccgccg atttcgcccc cgccctcgcc ccgcgcccga tgccgcccga gatggcgcat
     4261 gcgctgggcg cgcacttcgg ggctcgctac gtcacgtcga tggcggtgcg ggagcagcac
     4321 gggcgcgacg agtcgccgat cgatgcgccg ccgcccgagg ccgtcgtgtt ctgcgagagc
     4381 acggcggacg tcgccttcgt cgtcaagcag gccgcgacgc atcgcgtgcc ggtcatcccg
     4441 tacggcgcgg gttcgtcgct cgagggccac ctgctcgcgg tgcagggcgg cgtgagcgtc
     4501 gacgtctcgc gcatggacgc ggtgctgcag ctcaacgccg aggacctcac cgtgaccgtc
     4561 caggccggcg tgacgcgcat gcagctcaat cgcgagatca aggactcggg cctgttcttc
     4621 ccgatcgacc cgggcgcgga cgcgacgatc ggcggcatgt cggccacgcg cgcgtcgggc
     4681 acgaacgcgg tgcgctacgg cacgatgcgc gagaacgtgc tcgcgctgac ggtggtgacg
     4741 ccatcgggcg aggtgatccg caccggcacc cgcgcgcgca agtcgagcgc gggctacgac
     4801 ctcacgcggc tggtcgttgg cagcgagggc acgctgggcg tgatcaccga ggtcacgctg
     4861 cggctgcacc cgctgccgga ggcggtgtcg gcggcgatct gctcgttcgc cgacgtgcgc
     4921 gcggcggtcg acacggtcat cgagatcatc caggtcggcg tgccgatcgc gcgctgcgag
     4981 ctgctcgacg ccaatgccgt ccgggcggtc aacgcgcacg acaagctggg cctggccgag
     5041 acgccgatgc tgctgatgga gttccacggc agcgactccg gcgtagcgga gcaggcggcg
     5101 acggtgcagg cgctggccgc gggccacggc ggcaccgcgt tccagtgggc cacgacgccc
     5161 gaggaacgca cgcgcctgtg gacggcgcgc caccacgcct tcctctcggg gctgcagatg
     5221 cgccccggct gccgcgcggt cacgaccgac tgctgcgtgc cgatctcgcg cctggccgat
     5281 gcggtgaccc aggcgctcgc ggaagccgac gccgccggca tgccgcactt cctggtcggc
     5341 cacgtgggcg acggcaactt ccacgtcgcc tacctgatcg acccggacga gccgaaggat
     5401 cgcgcgaccg ctgagcgcct gaaccacgcg ctcgtgcagc gcgcgatcgc cgcgggcggc
     5461 acctgcacgg gcgagcacgg catcggcctg cacaagcagg gcttcctggt cgaggaggcc
     5521 ggcctcgggg cggtcgcgat gatgcgccag atcaagcgcg cactcgatcc cgacaacatc
     5581 atgaacccgg gcaagatctt cgagctttga cggacgcgcc cgtccttcag aagcgcttga
     5641 cgaagcgcct gcgcacctcg gcgaagccct cccgctcgta gaacgcgtgg gcgcgcgtgc
     5701 ggcgctcgtt gctggagagc tccacgtggc tgcatcccgc ggcgcgggcg cgatcctcgg
     5761 cggccgcgag cag
//
LOCUS       DASVLN010000591        10035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_192326, whole genome shotgun sequence.
ACCESSION   DASVLN010000591 DASVLN010000000
VERSION     DASVLN010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192326"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VF453_14175"
     CDS             complement(<1..644)
                     /locus_tag="VF453_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7688856.1"
                     /translation="MAARTNLDMDVLRTFVTGFELGSFARAAERLGRSQSAVSTQLRK
                     LEEQVGVPLVRKAGRGLALTPAGESFLGYARRLLELNDEAVESIRGADVEGWVRLGLP
                     QDFAESWLPAVLHRFARAHPRVRVEVQVDRSVALADKTIKGELDIALAWSDGGDGLHA
                     RHVADVPGRWIGRPDWPGVAALDGEPLPFAAFAPPCTFRSAAIAALDDAGIPWRL"
     gene            747..1142
                     /locus_tag="VF453_14180"
     CDS             747..1142
                     /locus_tag="VF453_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX7688857.1"
                     /translation="MPLVQVSLLKGKSPEHLRAIGDGVHRALVDAFGIPPDDRFQLFR
                     QFDRDEFVYDAGYLGIQRSDDVVFIHIVAGNWRDTAKKKALYRAIADNLHRAPGLRPE
                     DVQVVLSLNERADWSFGSGLASYVNEGAD"
     gene            1314..1895
                     /locus_tag="VF453_14185"
     CDS             1314..1895
                     /locus_tag="VF453_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX7688858.1"
                     /translation="MKVNLKRIIGPWAVGWALDKHMLRSTYVGDDANGRPKFDSLRSE
                     AGEAIFLLKYRSDWSQASRLADAIATNICANLDHVGFIVPMPASTPRPRQPVAEIARA
                     LSKVINRPVFENLLLKAPTNQRLKDLHGKQEKVAALGNSFSIHDEIRAQGAWNVLLVD
                     DLYDSGASMEAAATALSTYPKVRNVYVTACTWK"
     gene            1899..2465
                     /locus_tag="VF453_14190"
     CDS             1899..2465
                     /locus_tag="VF453_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688859.1"
                     /translation="MTIVFIAGSIAIKTLAPQVLERIDNVVSSRLSVIVGDADGADTA
                     IQTYLAASGAADVTVYCSGTSARNNVGGWPLRPVETKHRPGSRAFFTAKDVEMARSAD
                     FGLMIWDSKSTGTLSNVVELLGRGRKSVVFIEKEKSFLNVGSVDQLEQLVAHMTSSAR
                     AAADDKMKLAMRIDELRHRQGRMFEPHP"
     gene            complement(2475..2870)
                     /locus_tag="VF453_14195"
     CDS             complement(2475..2870)
                     /locus_tag="VF453_14195"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX7688860.1"
                     /translation="MPFIDVKIAGAPLAPEQVARIQHGITTLMAEVLHKVGPLVGVLV
                     HQVPAAGWSVGGVPVERAAQVDAIVSAGTNTAEQKGRFIAETHALLAAVLGPGLSEVS
                     YVVVHEVPKDSWGYGGLTQAQRAVRREAA"
     gene            3108..4022
                     /locus_tag="VF453_14200"
     CDS             3108..4022
                     /locus_tag="VF453_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688861.1"
                     /translation="MQITKQVDTAQVTFALDDDLSEPNQRLYVDEGTRWLRDSRRRLT
                     RTLAYATRALTEPTKVAGAFWEMFHVYLGGETLDPANEKLVAGELNPVLAAIGKTLKG
                     LSGDVTLADLGQMPGVAKLQFKCLHAVCWAKGVPSPQLAGLVFVDVAEMAGRYLKSLW
                     KDPGKGSGFMNPFGSAIHLNFAELLRQGVDRGSVVTLIHEGTHKFAQTTDEQYFPDEG
                     ASDLLDKALDMCRPMISNYAGFEQMLADARKIAYRSGPMHEAYGNVQKMAPEKARNNA
                     DGIANFVYEVSELPTTDELTRIIRAVMN"
     gene            complement(4066..4833)
                     /gene="blaOXA"
                     /locus_tag="VF453_14205"
     CDS             complement(4066..4833)
                     /gene="blaOXA"
                     /locus_tag="VF453_14205"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HEX7688862.1"
                     /translation="MLLGTSAAQADCTLLVDAATKRVLHEDGACDARLTPASTFKVAL
                     SLMGFDAGVLKSEHVPLLDWRPGDVDWNPAWKAPTDPARWISLSVVWYSVRTTHALGM
                     AQVRHYLDAFDYGNRDFSGRPGQPDGLDGAWIASSLRISPREQAAFLGRLVRRELPVS
                     AHAFDMTARVTDLGPLPGAPAWTIHGKTGAAAPHDAHDEPDWNHGIGWFVGWATRGGR
                     SVVFVRQIQDATERQPSPGLRAKAAFLEALPALLPRD"
     gene            complement(4983..8993)
                     /gene="purL"
                     /locus_tag="VF453_14210"
                     /gene_synonym="purI"
     CDS             complement(4983..8993)
                     /gene="purL"
                     /locus_tag="VF453_14210"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX7688863.1"
                     /translation="MSSSSKHLLHFEGGNALSAFRAKALLPQLQAVSARITGVAARHV
                     HWVWTDEAPAADARERLARLLEYGDPYAGPTAADADSVLVVAMPRLGTVSPWASKATD
                     IARNCGLVLHRVERVTEYRLTLKSGLLGGTKPLAADELAAAAALLHDRMTESVALERD
                     AARHLFDEQPAAPLAHVDVLGQGRTALERANGEFGLALSDDEIDYLVDAFRGLARNPS
                     DVELMMFAQANSEHCRHKIFNAKFVIDGQEQPSSMFGMIRNTEKLNPQHTIVAYSDNA
                     AVMEGHAIERWVAAPQGTDGPAAYAGRAETAHVLMKVETHNHPTAISPFPGASTGSGG
                     EIRDEGATGRGSKPKAGLAGFSVGNLNLPGTSEPWEKSPIGKPEHIASALQIMVDGPL
                     GAAAFNNEFGRPNLGGYFRVFEQEVAGVVRGYHKPIMIAGGLGAIRADLTHKIEFAPG
                     TLLIQLGGPGMRIGMGGGAASSMAAGTNTAALDFDSVQRGNPEIQRRAQEVINQCWQL
                     GEANPILAIHDVGAGGLSNAFPELADGAGTGARFDLRKVPLEESGLAPKEIWCNESQE
                     RYVLAIAPESLPLLESFAARERCPLSVVGRATAEKSLVLEDGADGERVIDMPMDVLLG
                     KPPKMVRDVARVERSAPALKLDGVTLEQAALDVLRHPTVASKRFLITIGDRTVGGLSH
                     RDQMVGPWQVPVADCAVTLADFKGFAGEAMSMGERTPLAAVDAPASGRMAVAEAITNL
                     LAAPIELKRVKLSCNWMAACGEPGEDAALYDTVRAVGMELCPALGVSVPVGKDSLSMR
                     TRWSEAGEARQVVAPVSLIVSAFASVADVRGTLTPQLVTDQGDTALILVDLGRGKHRL
                     GGSVLAQTLQQFGDAVPDLDDPQLLVGLVDAVNALRAQGALLAYHDRSDGGLWAAACE
                     MAFAGHVGLSLNVDMLVTEGDGVSDSRADAGDAKNWASQVSGRREEQTLKALFAEELG
                     ALLQVPAARRDEAMAVLRAHGLAAHSHVVGKPNDKRVVEVWRDAKPVFSRPLRELHQA
                     WDETSWRIAALRDNPACADSEHALAGAEDDPGLQVALTFDPAEDVAAPFLNLARPQVA
                     ILREQGVNSHLEMSHAMARGGFDTFDVHMSDLQSGRVSLAQFKGFVACGGFSYGDTLG
                     AGEGWARSILFNAQLAAQFEAFFARTDTFALGVCNGCQMLAALSPMIPGAQDWPKFVR
                     NRSEQFEARLSQVEVLESPSIFFTGMAGSRLPIAVAHGEGYADFSQRGNPAKVLRAMR
                     YVDHHGKPTEAYPLNPNGSPEGLTAVTTPDGRFTALMPHAERVARNVQMSWSGGDVSV
                     ASPWMRMFGNARKWVN"
     gene            9095..9937
                     /gene="map"
                     /locus_tag="VF453_14215"
     CDS             9095..9937
                     /gene="map"
                     /locus_tag="VF453_14215"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7688864.1"
                     /translation="MTISLKSAADIEGMRIAGRLGSEVLDMLTPHVKPGITTEQLDKL
                     AHDYIVDVQGAVPAPLNYCPPGYTPYPKSICTSINHQICHGIPNARALKDGDIVNIDV
                     TVIKDGWHGDTSRMFVVGKGSIAANRLCQITYEAMWKGIEKVKPGARLGDIGHAIQVF
                     AENAGYSVVREFCGHGIGRKFHEEPQVLHYGRPGTNEELVPGMIFTIEPMINAGKRDI
                     KEMGDGWTIVTKDHSLSAQWEHTVVVTETGYEVLTVSAGSPAVPAFVHNPVLNRLMSE
                     AGAA"
BASE COUNT         1551 a         3541 c         3514 g         1429 t
ORIGIN      
        1 agccgccagg ggatgcccgc gtcgtccagc gccgcgatgg ccgccgaacg gaaggtgcac
       61 ggcggcgcga aggccgcgaa cggcagcggc tccccgtcca gcgcggcgac gccgggccaa
      121 tcgggccggc cgatccagcg gccggggacg tcggcgacat gccgcgcatg caggccgtcg
      181 ccgccgtcgc tccaggcgag cgcgatgtcg agctcgccct tgatcgtctt gtccgcgagc
      241 gcgacgctgc ggtcgacctg cacctcgacg cgcacccgcg ggtgcgcacg cgcgaagcgg
      301 tggagcaccg cgggcagcca gctctcggcg aagtcctgcg gcaggcccag gcgcacccag
      361 ccttcgacat cggcgccgcg gatgctctcg acggcctcgt cgttgagctc cagcagccgc
      421 cgggcgtagc cgaggaagct ctcgccggcg ggcgtcagcg cgagcccgcg tcccgccttg
      481 cgcaccagcg gcacgccgac ctgctcctcg agcttgcgca gctgcgtgct gaccgccgac
      541 tgcgagcgcc ccagccgctc ggcggcgcgc gcgaagctgc cgagctcgaa cccggtgacg
      601 aaagtgcgca gcacgtccat gtcgaggttg gtgcgggccg ccatagttcg attctccgaa
      661 ttcgtctctt ccgaactttc gacttttcag aagcattatc ggcgcctaag ctggtgtccg
      721 tcaaagtcaa ccacggagcc cgcaccatgc ctctcgtcca agtctccctg ctcaagggca
      781 agtcgccgga acacctgcgc gccatcggcg acggcgtcca ccgggcgctg gtcgacgcgt
      841 tcggcatccc gcccgacgac cgcttccagc tgttccgcca gttcgatcgc gacgagttcg
      901 tctacgacgc cggctacctc ggcatccagc gcagcgacga cgtcgtcttc atccacatcg
      961 tggccggcaa ctggcgcgac acggccaaga agaaggcgct gtaccgggcc atcgcagaca
     1021 acctccaccg cgccccgggc ctgcggccgg aggacgtgca ggtcgtgctg tcgctcaacg
     1081 agcgcgccga ctggtcgttc ggcagcgggc tggcgtcgta cgtgaacgaa ggggccgact
     1141 gagcgccgac cggcacttcc agctcgaatc ggcgcctcga ttcccgcatg tgcagcgccg
     1201 tgcgcagccg cagccccctc ttcatgagga tgttcggcgt cccacgagtg cacatacgct
     1261 tgccggcata ccaaccgcgc gcgtcgacga ggcaaccgca aagggagagg cgagtgaaag
     1321 tcaatctgaa gcgcatcatc gggccgtggg cagtgggctg ggcgctggac aagcacatgt
     1381 tgcgcagcac ctatgtcgga gacgacgcca acgggcgtcc gaaattcgac agccttcgca
     1441 gcgaggcggg cgaggcgatc ttcctgctga aatacagaag cgattggtcc caagcctctc
     1501 gactggccga tgccatcgcc acgaacatat gcgccaacct ggatcacgtc ggtttcatag
     1561 tgcccatgcc cgccagtacg ccgaggccac gccagcccgt ggccgagatc gcacgcgcgc
     1621 tgtcgaaagt catcaaccgg ccggtcttcg aaaatttgct tctcaaggcg cccaccaatc
     1681 aacgactcaa ggatctgcat gggaagcagg agaaggtcgc ggcgctgggc aacagcttca
     1741 gcatccatga tgaaataaga gcccaaggcg cctggaacgt cctactcgtc gacgacctct
     1801 acgactccgg tgcatcgatg gaagccgcgg cgacagcgct gagcacctat cccaaggttc
     1861 gaaacgtgta tgtcacggca tgcacgtgga agtagtcaat gaccatcgtc ttcatcgccg
     1921 gctccatcgc catcaagaca ctggcaccgc aggtcctcga acgcatcgac aacgtcgtct
     1981 cttcgaggct gtcagtcatc gtcggggatg cggatggcgc cgacacggcc atccagacgt
     2041 acctggccgc ctccggcgca gcggacgtga cggtctattg cagcggcacg tccgcccgca
     2101 acaatgtggg tggatggccg ctcaggcctg tcgagacgaa gcatcgtccg ggctcgcgcg
     2161 ctttcttcac agccaaggat gtggagatgg cgcggtccgc ggatttcggc ctcatgatct
     2221 gggacagcaa gagcaccggg acactgagca acgtcgtcga attgctgggc cgcggtagga
     2281 agtctgtcgt cttcatcgag aaggagaaga gcttcctgaa cgttggctcg gtggatcagc
     2341 tcgaacaact cgttgcccac atgacgtcgt cggcccgcgc ggccgccgac gacaagatga
     2401 agctggcgat gcggatcgac gaacttcggc accgtcaggg acgcatgttc gaaccgcacc
     2461 cgtgacccgc cgtctcaggc tgcctcacgc cggacggccc gctgcgcctg cgtgagcccc
     2521 ccgtagcccc agctgtcctt gggcacctcg tgcaccacga cgtacgagac ctcggacagc
     2581 cccggcccca gcacggcggc cagcagcgcg tgcgtctcgg cgatgaagcg gcccttctgc
     2641 tccgcggtgt tggtgccggc gctgacgatc gcgtcgacct gcgccgcgcg ttcgacaggc
     2701 acgccgccga cgctccatcc cgcggcgggc acctggtgca cgagcacgcc gaccagcgga
     2761 ccgaccttgt gcagcacctc cgccatcagc gtggtgatcc cgtgctggat gcgggcgacc
     2821 tgttccggcg cgagcggcgc gccggcgatc ttgacgtcga tgaaaggcat gtcctgggct
     2881 ccttgcgatc cgcgccgccc cctgcgggcg acgccatgga aggcacttta ggagtcgcgg
     2941 acgcttttga aaattcgaaa gtttgcaatc gatgcatcgg agggatcgaa cgatcgccgg
     3001 cgggcaggcg gggtaggtca agcccgggtt gccaggcccg gccaaggcgc gagactgcgc
     3061 gggccgcccc gcggcgccaa ccattcattc caggggacaa ggacgccatg cagatcacca
     3121 agcaagtgga caccgcgcaa gtcacgttcg cgctggacga cgacctgagc gaaccgaacc
     3181 agcgcctcta cgtcgacgag ggcacgcgct ggctgcgcga ctcgcgccgc cggctcaccc
     3241 gcacgctcgc ctacgccacc cgcgcgctga ccgagccgac caaggtcgcg ggcgccttct
     3301 gggagatgtt ccacgtctac ctgggcggcg agacgctcga tcccgccaac gagaagctgg
     3361 tcgccggcga gctcaacccg gtgctggccg cgatcggcaa gacgctcaag ggcctgagcg
     3421 gcgacgtgac cctcgcggac ctgggccaga tgccgggcgt ggccaagctc cagttcaagt
     3481 gcctgcacgc ggtgtgctgg gccaagggcg tgccgtcgcc gcagctggcg ggcctggtgt
     3541 tcgtggacgt cgccgagatg gccggccgct acctcaagag cctgtggaag gatcccggca
     3601 agggctcggg cttcatgaat ccgttcggca gcgccatcca cctgaacttc gccgagctgc
     3661 tcaggcaggg cgtcgaccgc ggcagcgtgg tgaccctcat ccacgaaggc acgcacaagt
     3721 tcgcccagac caccgacgag cagtacttcc ccgacgaagg cgccagcgac ctgctggaca
     3781 aggcgctcga catgtgccgg ccgatgatct ccaactacgc gggtttcgag cagatgctcg
     3841 cggacgcgcg caagatcgcc taccgcagcg gcccgatgca cgaggcctac ggcaacgtgc
     3901 agaagatggc gcccgagaag gcacgcaaca acgccgacgg catcgcgaac ttcgtctacg
     3961 aggtctccga gctgccgacc acggacgagc tcacgcgcat catccgtgcg gtgatgaact
     4021 gagcggggca gcgcgggcgg gggatgccgc gcgctccccg gcgggtcagt cgcgcggcaa
     4081 cagggccggc agcgcctcca ggaacgccgc cttggcccgc aaccccggtg aaggctgccg
     4141 ctcggtcgcg tcctggatct gccgcacgaa gacgacgctg cggccgccgc gcgtcgccca
     4201 gccgacgaac cagccgatgc catggttcca gtccggttcg tcgtgcgcgt cgtgcggcgc
     4261 ggcggcgccg gtcttgccgt ggatcgtcca ggccggcgca ccgggcagcg ggccgaggtc
     4321 cgtgacgcgc gcggtcatgt cgaacgcgtg cgcggacacg ggcagctcgc gccgcaccag
     4381 cctgccgagg aaggccgcct gctcgcgcgg cgagatgcgc agcgacgacg cgatccaggc
     4441 gccgtcgagg ccgtcgggct gcccgggccg gccggagaag tcgcggttgc cgtagtcgaa
     4501 cgcgtcgagg tagtgccgca cctgggccat cccgagcgca tgcgtggtgc gcaccgaata
     4561 ccagacgacc gacagcgaga tccagcgcgc cggatcggtc ggcgccttcc aggccgggtt
     4621 ccagtcgacg tcgccgggac gccagtccag cagcgggacg tgctcggact tcagcacccc
     4681 cgcatcgaag cccatcaggc tcagcgccac cttgaaggtc gaggcgggcg tcaggcgcgc
     4741 gtcgcaggcg ccgtcctcgt gcagcacgcg cttcgtggcg gcgtcgacga gcagcgtgca
     4801 gtcggcctgc gcggccgacg tgccgagcag caatgccagc gcggcagccc gggaggctcg
     4861 ccacctccgg cgctgccgcc ctgacgtacc aggcgagacg cggggaagcg gcggatccgg
     4921 ctccgccggg ccgctcgcgc acggccccct cggggggcag ggagcgccag cgaccgtggg
     4981 ggctagttca cccacttgcg cgcgttgccg aacatccgca tccacgggct cgcgaccgac
     5041 acgtcgccgc cgctccaaga catctgcacg ttgcgcgcga cccgttcggc gtgcggcatg
     5101 agcgcggtga agcggccgtc gggggtcgtg accgcggtca ggccctcggg gctgccgttc
     5161 gggttgagcg ggtaggcctc ggtgggcttg ccgtggtggt cgacgtagcg catcgcgcgc
     5221 agcaccttgg cggggttgcc gcgctgggag aagtcggcgt agccctcgcc gtgcgccacg
     5281 gcgatgggca ggcggctgcc ggccatgccg gtgaagaaga tcgacggcga ctcgagcacc
     5341 tcgacctgcg acagccgggc ctcgaactgc tcgctgcggt tgcgcacgaa cttgggccag
     5401 tcctgcgcgc ccgggatcat cggcgacagc gcggccagca tctggcagcc gttgcacacg
     5461 ccgagcgcga aggtgtcggt gcgcgcgaag aaggcctcga actgcgccgc gagctgcgcg
     5521 ttgaacagga tcgagcgcgc ccagccctcg ccggcgccca gcgtgtcgcc gtagctgaag
     5581 cccccgcagg cgacgaagcc cttgaactgc gcgagcgaga cgcggcccga ctgcaggtcg
     5641 ctcatgtgga cgtcgaaggt gtcgaagccg ccgcgtgcca tcgcgtggct catctcgagg
     5701 tggctgttga cgccctgctc gcgcaggatg gcgacctgcg ggcgcgccag gttcaggaac
     5761 ggcgcggcga cgtcctcggc cggatcgaag gtgagcgcca cctgcaggcc cggatcgtcc
     5821 tcggcgccgg ccagcgcgtg ctcgctgtcg gcgcaggcgg ggttgtcgcg cagcgcggcg
     5881 atgcgccagc tcgtctcgtc ccaggcctgg tgcagctcgc gcagggggcg cgagaagacc
     5941 ggcttcgcgt cgcgccagac ctcgacgacg cgcttgtcgt tgggcttgcc gaccacgtgg
     6001 ctgtgcgcgg ccaggccgtg cgcgcgcagc acggccatcg cctcgtcgcg gcgggcggcg
     6061 ggcacctgca gcagcgcgcc gagctcctcg gcgaacagcg ccttgagcgt ctgctcctcg
     6121 cggcggccgc tgacctggct cgcccagttc ttcgcgtcgc cggcgtcggc gcggctgtcg
     6181 gagacgccgt cgccctcggt gaccagcatg tccacgttca ggctcaggcc gacgtggccg
     6241 gcgaacgcca tctcgcacgc ggccgcccac aggccgccgt cgctgcggtc gtggtaggcg
     6301 agcagcgccc cctgcgcgcg cagcgcgttg acggcgtcga cgaggccgac cagcagctgc
     6361 gggtcgtcga ggtcgggcac cgcgtcgccg aactgctgca gcgtctgcgc gagcaccgag
     6421 ccgccgaggc gatgcttgcc gcggcccagg tcgacgagga tcagcgcggt gtcgccctgg
     6481 tcggtgacga gctgcggcgt gagcgtgccg cgcacgtcgg cgacgctcgc gaaggccgaa
     6541 acgatcagcg agaccggcgc gacgacctgc ctcgcctcgc ccgcctcgct ccaacgcgtg
     6601 cgcatcgaca ggctgtcctt gcccaccggg acgctcacgc ccagcgcggg gcagagctcc
     6661 atgccgacgg cgcgcaccgt gtcgtagagc gccgcgtcct cgccgggctc gccgcaggcg
     6721 gccatccagt tgcagctgag cttgacgcgc ttcagctcga tcggcgccgc cagcaggttg
     6781 gtgatggcct cggccaccgc catgcggccc gacgcgggcg cgtcgacggc cgcgagcggc
     6841 gtgcgctcgc ccatgctcat cgcctcgccg gcgaagccct tgaagtcggc cagcgtgacc
     6901 gcgcagtcgg ccaccggcac ctgccacggg ccgaccatct ggtcgcggtg cgacaggccg
     6961 ccgaccgtgc ggtcgccgat cgtgatcagg aagcgcttgg aggcgacggt gggatgacgc
     7021 agcacgtcga gcgcggcctg ctccagcgtc acgccgtcca gcttcagcgc cggcgccgag
     7081 cgctcgacgc gggcgacgtc gcgcaccatc ttcggcggct tgcccagcag cacgtccatc
     7141 ggcatgtcga tgacgcgctc gccgtccgcg ccgtcctcca gcaccagcga cttctcggca
     7201 gtcgcgcggc cgacgacgga cagcgggcag cgctcgcgcg cggcgaagga ctcgagcagc
     7261 ggcagcgact ccggcgcgat cgcgaggacg tagcgctcct ggctctcgtt gcaccagatc
     7321 tccttgggcg cgaggccgga ttcctccagc ggcaccttcc tcaggtcgaa gcgcgcgccg
     7381 gtgcccgcgc cgtcggcgag ctcggggaag gcgttggaca gcccgcccgc gccgacgtcg
     7441 tggatcgcca ggatcgggtt ggcctcgccc agctgccagc actggttgat gacctcctgc
     7501 gcgcggcgct ggatctcggg gttgccgcgc tgcacggagt cgaagtccag cgccgcggtg
     7561 ttggtgccgg cggccatcga gctcgccgcg ccgccgccca tgccgatgcg catgccgggg
     7621 ccgccgagct ggatcagcag cgtgccgggc gcgaactcga tcttgtgcgt caagtcagcg
     7681 cggatggcgc ccaggccgcc cgcgatcatg atcggcttgt ggtagccgcg caccacgccg
     7741 gcgacctcct gctcgaacac gcggaagtag ccgcccaggt tgggccggcc gaactcgttg
     7801 ttgaaggcgg cggcgccgag cgggccgtcg accatgatct gcagcgcgct ggcgatgtgc
     7861 tccggcttgc cgatcgggga cttctcccac ggttcgctgg tgcccggcag gttcaggttg
     7921 ccgaccgaga agcccgccag gcccgccttg ggcttggagc cgcggccggt cgccccctcg
     7981 tcgcggatct cgccgccgga gccggtggac gcgcccggga acggcgagat ggccgtcggg
     8041 tggttgtgcg tctccacctt catcagcacg tgcgcggtct cggcgcggcc ggcataggcg
     8101 gccggcccgt ccgtgccctg cggcgcggcg acccagcgct cgatcgcgtg gccctccatc
     8161 accgcggcgt tgtcggagta ggcgacgatc gtgtgctgcg ggttgagctt ctcggtgttg
     8221 cggatcatgc cgaacatcga gctcggctgc tcctggccgt cgatcacgaa cttcgcgttg
     8281 aagatcttgt ggcggcagtg ctcgctgttg gcctgcgcga acatcatcag ctcgacgtcg
     8341 ctcgggttgc gcgcgaggcc gcggaaggcg tcgaccaggt aatcgatctc gtcgtccgac
     8401 agcgccagcc cgaactcgcc gttggcgcgc tccagcgccg tgcggccctg gccgagcacg
     8461 tcgacgtggg ccagcggcgc ggcgggctgc tcgtcgaaga ggtggcgggc ggcgtcgcgc
     8521 tccagggcca cgctctcggt catgcggtcg tgcagcagcg cggcggcggc ggcgagctcg
     8581 tcggccgcga gcggcttggt gccgcccagc aggccggact tcagcgtcag gcggtattcc
     8641 gtcacgcgct cgacgcggtg cagcacgagc ccgcagttgc gggcgatgtc ggtcgccttg
     8701 ctggcccagg gcgagacggt gcccaggcgc ggcatggcga cgacgagcac ggagtcggcg
     8761 tccgcggcgg tcgggccggc gtaggggtcg ccgtactcca gcaggcgggc caggcgctcg
     8821 cgggcgtcgg cggcgggcgc ctcgtccgtc cagacccagt gcacgtgccg cgcggcgacg
     8881 cccgtgatgc gcgcggagac cgcctgcagt tgcggcagca gcgccttggc gcggaacgcg
     8941 gacagggcgt tgccgccctc gaagtgcagc aggtgcttgg agctggaaga catcgggtgt
     9001 gggaggtgtg cggggccggg agactgcggg cgtcgcgggg gaacgacatc ggcgaaacca
     9061 cggattttac ggggcgatgg gataattgcc caccatgacg atctccctga agtccgctgc
     9121 cgacatcgaa ggcatgcgca tcgccggccg cctcggctcc gaggtgctgg acatgctcac
     9181 cccgcacgtg aagcccggca tcacgacgga gcagctcgac aagctcgcgc acgactacat
     9241 cgtcgacgtg cagggcgccg tccccgcccc gctgaactac tgcccgcccg gctacacgcc
     9301 ctacccgaag tcgatctgca cgtcgatcaa ccaccagatc tgccacggca tccccaacgc
     9361 ccgggcgctg aaggacggcg acatcgtcaa catcgacgtg acggtgatca aggacggctg
     9421 gcacggcgac acgagccgca tgttcgtggt cggcaagggc tccatcgccg ccaaccgcct
     9481 gtgccagatc acctacgagg cgatgtggaa gggcatcgag aaggtgaagc cgggcgcgcg
     9541 cctgggcgac atcggccatg cgatccaggt cttcgcggag aacgccggct actcggtcgt
     9601 gcgcgaattc tgcggccacg gcatcggccg caagttccac gaggagccgc aggtcctgca
     9661 ctacggccgt cccggcacga acgaggagct ggtgcctggc atgatcttca cgatcgagcc
     9721 gatgatcaac gccggcaagc gcgacatcaa ggagatgggc gacggctgga ccatcgtcac
     9781 gaaggaccac tcgctgtcgg ctcagtggga gcacacggtc gtcgtcaccg agaccggcta
     9841 cgaggtgctg accgtctcgg ccggcagccc ggcggtgccg gcgttcgtgc acaacccggt
     9901 gctcaaccgg ctcatgagcg aggctggcgc cgcctgaggg cgcgcgccga cccgccggtt
     9961 gaccgccgac cgcatgaccg cctccgtcgc cgcgccccag gggccggccg cgtccgccca
    10021 gcgccccacc ctgcc
//
LOCUS       DASVLN010000592         4240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_462288, whole genome shotgun sequence.
ACCESSION   DASVLN010000592 DASVLN010000000
VERSION     DASVLN010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462288"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VF453_14220"
     CDS             complement(<1..678)
                     /locus_tag="VF453_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688865.1"
                     /translation="MTTAAFRHPSSPIGACVLGIAVALSTGVGVAQAQTAGSVSSVPS
                     VQKGTTGRQLWRLSDFSTVELVAIEPGAPANQHPWETDASALRTMLQQVTITKGKGTK
                     PLFGSEELGNLVPALVEAFANARPDQDVAVISSARHDDNSFYGVSAVTARLFFVGGHL
                     NMIVHDPRYDFYDSARGMGVAPHFTIGSRTEAGVAPVYSPTATSSRPDWLVLNAAPAP
                     AAVGVPAT"
     gene            complement(928..1956)
                     /gene="murB"
                     /locus_tag="VF453_14225"
     CDS             complement(928..1956)
                     /gene="murB"
                     /locus_tag="VF453_14225"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX7688866.1"
                     /translation="MSLDSWLESDVSLKALNTFGLPAHARRLARIRADADVRRVLDSP
                     EVGRAAKLILGGGSNLVLTRDVDEVVLKVEVPGLRLVEARDDAFIVEAGAGMAWHDVV
                     TQTLEQGWPGLENLALIPGTVGAAPVQNIGAYGLELADRFDSVEGVDLVTGRGVTLDR
                     ELCQFGYRDSVFKHVLAGKVVITRVRLRLPRPWRPVLGYLELERKVAETGIADPGPRQ
                     VYDWICAIRRAKLPDPARIGNAGSFFKNPIVSEDQCRDIIGRDPHVVHYPMPDGSFKL
                     AAGWLIDACGWKGKSIGRAGVYEKQALVLVNRGDAIGAEVMTLARAIQESVYGRFGIR
                     LEPEPVVL"
     gene            2013..2507
                     /locus_tag="VF453_14230"
     CDS             2013..2507
                     /locus_tag="VF453_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX7688867.1"
                     /translation="MPSFDAVLEPDMVAIKHSVENSRKEIGTRFDFKGTSAAVELKEK
                     EKEIQLVGDGDFQIEQINAVLIGKLTKQGVAVSYLDMTGKIEKIGGDKVRQTHKIKAG
                     IESTLAKKIVSAIKDAKLKVQASIQGDTVRVTGKNRDDLQLAIAQLRKDFPDQPLKYE
                     NFRD"
     gene            2844..4025
                     /locus_tag="VF453_14235"
     CDS             2844..4025
                     /locus_tag="VF453_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7688868.1"
                     /translation="MDLDFTPDQQAFRADVRAWVSANLPADISHKVHHALRLTRDDMQ
                     RWAKILGAKGWLGYAWPKEFGGPGWDAIQRHLFEEECALAGAPRIVPFGPVMVAPVIM
                     AFGTPEQQRRFLPGIASGEVWWSQGYSEPGSGSDLASVKTRAERRGDKYVVNGQKTWT
                     TLGQYGEWIFCLVRTSTEGKPQTGISFLLIDMKSPGVTVRPIVLMDGEPEVNEVFFDD
                     VEVPVENLVGEENKGWTYAKYLLAHERTNIADVNRAKRELERLKRIAKAEGVYDDPRF
                     RDQVALLEVDVVALEMMVLRVLSAQAGGKKALDVAGLLKIRGSEIQQRYTELLMLAGG
                     PFSLPFISEAMDAGWQGDHVGAAHCAPLAANYLNYRKTTIYGGSNEVQRNIVAQTVLG
                     V"
     gene            4027..>4240
                     /locus_tag="VF453_14240"
     CDS             4027..>4240
                     /locus_tag="VF453_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7688869.1"
                     /translation="MNFDFSDDEQQLSDAVRKWVDRGYGFERRRAIEKSGGFSAEAWS
                     EIAELGLLGLGTSEDDGGMALGPVAAM"
BASE COUNT          702 a         1462 c         1477 g          599 t
ORIGIN      
        1 ggttgccgga acgcccacgg cggctggcgc gggggcggca ttcaacacga gccagtcagg
       61 gcgcgagctg gtggcggtcg ggctgtacac cggcgcgacg cccgcctccg tgcgcgagcc
      121 gatcgtgaag tgcggcgcga cgcccatgcc gcgcgcgctg tcgtagaagt cgtagcgcgg
      181 atcgtggacg atcatgttca gatggccgcc gacgaagaac agtcgcgcag tgaccgcgct
      241 cacgccgtag aaactgttgt cgtcgtggcg ggcgctgctg atcaccgcga cgtcctggtc
      301 gggccgcgca ttcgcgaagg cctcgaccag cgccggcacg aggttgccga gctcctcgct
      361 gccgaacagc ggcttcgtgc ccttgccctt ggtgatcgtg acctgctgca gcatggtgcg
      421 cagcgcggag gcgtcggtct cccagggatg ctggttggcg ggcgccccgg gttcgatggc
      481 gacgagctcg acggtcgaga aatcgctcag gcgccagagc tgccgccccg tcgtcccctt
      541 ctgcacgctc ggcacggagg agaccgaacc cgcggtctgc gcctgcgcga cgccgacgcc
      601 agtcgacagc gcgacggcga tgccgaggac gcaggcgccg atcgggctgg aggggtggcg
      661 gaaggcggcg gtggtcatcg agacggtctc caggattgtt cgaatgcggc cccggcaggc
      721 gggttgctgg gcgtgaactc taacgcagcc gcgacggccg cgcctcgccg cacttgcggt
      781 cgcgcttgcg ggccgcgctc accgacgctg ccgcgcaagc gcgatctcgt ccccgccatt
      841 cggcaccctg gtgaccggca tcgccgaagc ctccgtgcgc cgccggttcc gtcgcggcac
      901 cgcctcccgc gttgccgccg ggccgcgtca gagcacgacc ggctcgggct ccaggcggat
      961 gccgaaacgg ccgtacacgc tctcctggat cgctcgcgcc agcgtcatca cttcggcacc
     1021 gatcgcgtcg ccgcggttga ccagcaccag cgcctgcttc tcgtacacgc ccgcgcggcc
     1081 gatcgacttg cccttccagc cgcacgcgtc gatgagccag ccggccgcga gcttgaagct
     1141 gccgtcgggc atcgggtagt gcacgacatg gggatcgcgg ccgatgatgt cgcggcactg
     1201 gtcctcgctg acgatcggat tcttgaagaa gctgccggcg ttgccgatcc gtgcggggtc
     1261 cgggagcttg gcccgccgga tcgcgcagat ccagtcgtag acctggcgcg gtccgggatc
     1321 ggcgatgccc gtttccgcga ccttgcgttc gagctcgaga tagcccagca cgggccgcca
     1381 gggacgcggc aggcgaagtc gtacccgcgt gatgacgacc ttgcccgcca ggacgtgctt
     1441 gaagaccgag tcgcggtagc cgaactggca cagctcgcgg tcgagcgtga cgccgcggcc
     1501 ggtgacgagg tccaccccct cgacggagtc gaagcgatcc gccagctcga ggccgtaggc
     1561 gccgatgttc tgcaccggcg ccgccccgac cgtgcccggg atcagcgcga ggttctcgag
     1621 cccgggccag ccctgctcga gcgtctgcgt gacgacgtcg tgccacgcca tgccggcacc
     1681 ggcctcgacg atgaaggcgt cgtcgcgcgc ctcgaccagt cgcaggcccg gcacctcgac
     1741 cttgagcacg acctcgtcga cgtcgcgcgt cagcacgagg ttgctgccgc cgccgaggat
     1801 cagcttggcg gcgcgtccga cctcgggcga atcgagcacg cgccgcacgt cggcgtcggc
     1861 gcggatgcgc gcgagccggc gcgcatgcgc gggcaggccg aaggtgttca atgccttcag
     1921 cgagacgtcg gattcgagcc aggagtcgag ggacatggca gaattttcgc agacccgttt
     1981 ccctgaccca gcccgttcct cggatttctc tcatgcccag cttcgacgcc gttctcgaac
     2041 ccgacatggt cgccatcaag cactcggtgg aaaactcgag gaaggagatc ggtacccgct
     2101 tcgacttcaa gggcaccagc gccgcggtgg agctcaagga gaaggaaaag gagatccagc
     2161 tcgtcggcga cggcgacttc cagatcgagc agatcaatgc cgtgctgatc ggcaagctca
     2221 ccaagcaggg cgtggccgtc agctacctcg acatgaccgg caagatcgag aagatcggcg
     2281 gcgacaaggt ccgccagacg cacaagatca aggccggcat cgagagcacg ttggccaaga
     2341 agatcgtcag cgcgatcaag gacgccaagc tcaaggtgca ggcgagcatc cagggcgaca
     2401 ccgtgcgcgt cacgggcaag aaccgcgacg acctgcagct cgcgatcgcc cagctgcgca
     2461 aggacttccc cgaccagccg ctcaagtacg agaacttcag ggactgacgg cgtcgtccgc
     2521 gacacgccag tcgcgccatg cgcgcccggc cgccgtggcc acggctcctg caccgaacgc
     2581 caccgcaggg tactgcgcga gcgtgaacag cccgagcagc gcgccgccca cgccggctcc
     2641 ggcacgcgcg gcggcacggg ggggtgacca cgacgccgcc ttccggcacc gtctgcggcc
     2701 gctcgacgcg gcgctcgaga cactgcccgg agcgcctgcc cgtggcctcg gggtcttccc
     2761 ggcctgtcgc actctggaca gcgaaaattc gaacgctcgt gctaaaacaa gcaccgcgcc
     2821 atccggcgca ggagacagcg tccatggatc tcgatttcac ccccgaccag caggccttcc
     2881 gcgccgacgt gcgggcctgg gtgtcggcga acctgccggc cgacatctcg cacaaggtgc
     2941 accacgccct gcggctcacg cgcgacgaca tgcagcgctg ggcgaagatc ctcggggcga
     3001 agggctggct gggctacgcc tggcccaagg aattcggcgg ccccggctgg gacgcgatcc
     3061 agcgccacct gttcgaggag gaatgcgccc tggccggcgc gccgcgcatc gtgcccttcg
     3121 gccccgtgat ggtggcgccc gtgatcatgg ccttcggcac gccggagcag caacgccgct
     3181 tcctgcccgg catcgcctcg ggcgaggtgt ggtggagcca gggctacagc gagccgggct
     3241 cgggctccga cctcgcgtcg gtcaagaccc gcgccgaacg ccggggcgac aagtacgtcg
     3301 tcaacggcca gaagacctgg acgaccctgg gccagtacgg cgagtggatc ttctgcctcg
     3361 tgcgcacctc gaccgagggc aagccgcaga cgggcatctc cttcctgctg atcgacatga
     3421 agtcgcccgg cgtgaccgtg cggcccatcg tgctgatgga cggcgagccc gaggtcaacg
     3481 aggtcttctt cgacgacgtc gaggtgccgg tcgagaacct cgtcggcgag gagaacaagg
     3541 gctggacgta cgcgaaatac ctgctcgccc acgagcgcac caacatcgcc gacgtcaacc
     3601 gcgccaagcg cgagctcgag cggctcaagc gcatcgcgaa ggcggaaggc gtctacgacg
     3661 acccgcgctt ccgcgaccag gtcgcgctgc tggaggtcga cgtggtcgcg ctcgagatga
     3721 tggtgctgag ggtgctcagc gcccaggccg ggggcaagaa ggcgctggac gtcgcgggcc
     3781 tgctcaagat ccgcggcagc gagatccagc agcgctacac ggagctcctg atgctggcgg
     3841 gcggcccgtt cagcctgccg ttcatctccg aggcgatgga cgccggctgg cagggtgacc
     3901 atgtcggcgc cgcgcactgc gcgccgctgg cggccaacta cctcaactat cgcaagacga
     3961 cgatctacgg cggatccaac gaagtgcagc gcaacatcgt cgcccagacg gtgcttggag
     4021 tgtgagatga atttcgactt cagcgacgac gaacagcaac tgagcgacgc ggtgcgcaag
     4081 tgggtcgacc gcggctacgg cttcgagcgc cggcgcgcga tcgagaagtc gggcggcttc
     4141 agcgccgagg catggagcga gatcgcggag ctgggcctgc tcgggctcgg cacctcggag
     4201 gacgacggcg gcatggcgct cgggccggtc gcggcgatgc
//
LOCUS       DASVLN010000593         7266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_245797, whole genome shotgun sequence.
ACCESSION   DASVLN010000593 DASVLN010000000
VERSION     DASVLN010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7266
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245797"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VF453_14245"
     CDS             <1..738
                     /locus_tag="VF453_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX7688870.1"
                     /translation="DARLAAFARWTNLSETTFLLPPTSPEADYRVRIFTPGGELPFAG
                     HPTLGSCHAWLAAGGRPRRDDVVVQECGVGLVPIRRLPRLAFAAPPAAFEPVDAALLQ
                     RVCAALGLDPARVRDATWADNGPRQLTLLVDAAATVLALRPDHAALKTLAKVGVVGPH
                     PDGAECRFEVRFFAAAIGVNEDPVTGSLNAKIAQWLIGSGRAPASYVAAQGTAMGRAG
                     RVHVQRDADGMWIGGDVAPCVSGTVLL"
     gene            797..1537
                     /locus_tag="VF453_14250"
     CDS             797..1537
                     /locus_tag="VF453_14250"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7688871.1"
                     /translation="MSRIVESPLHYGAAGRAYRGLLTARDDGTPAKAAVVLLPDWRGQ
                     SDLARDHARVLVDAGCAVAVADLYGDGFSPTDPAQVGPMVKRLMEHREDGVAALSACV
                     EALSERVGASVPVFCLGYSAGGMVALDYGRSGAKVAGIIVCSALLKTAAPGMSTRIAA
                     PVLMLQGTRDEVSPVSTIAEVIAEMDAAGNRFRIHLYGQTHHAFDNPQAGTDPTARLV
                     YSPESARLARRTIAEFVAEQAGAAGRST"
     gene            1545..2255
                     /locus_tag="VF453_14255"
     CDS             1545..2255
                     /locus_tag="VF453_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX7688872.1"
                     /translation="MTEELFRDDATLAECEARVLAADARGVQLDRTVFYPQGGGQAGD
                     RGELVLPDGRAVAVVDTRKGEAPGEIVHVLANPEDAAALAPGQAVRARIDVARRRAHM
                     RFHTATHLLCALVPHPVDGCSITAEYARLDFHMTDALDKAALEDGLARLVAAAHPVSH
                     RWISDAELDANPQLVRSMSVQPPRGTGRVRLLEIEGVDLQPCGGTHVANTSEIGAIGV
                     RKIEKKSAQTRRVVLEFR"
     gene            2281..2808
                     /locus_tag="VF453_14260"
     CDS             2281..2808
                     /locus_tag="VF453_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7688873.1"
                     /translation="MEAFAIRPFSRDDDSLVDLTALLHRAYAPLGAMGLNYTAVDQPP
                     EKTAQRLGSGTCFVATDGVALVGTILVHPTYDANDCDYFTRPGVACVHQFAVEPSLQG
                     AGVGRALLRHAEDWARAAGFREVAMDTAEPATHLVEFYARQGYERVGHVRWPGKVYRS
                     VVLAKRLADEGVRAA"
     gene            complement(2836..3741)
                     /locus_tag="VF453_14265"
     CDS             complement(2836..3741)
                     /locus_tag="VF453_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7688874.1"
                     /translation="MRHASTLVTAAGGPAFSRIVAGAWRMDDWGLDVAGRRRWIEGCL
                     DLGITTFDHADIYGGYRVEALFGEALAAAPGLRDRLQIVTKCGIRMVSPARPAHRIGH
                     YDTSAAHIEASVDRSLANLRTDRLDVLLIHRPDALMDADEVAACFERLRAAGKVLHFG
                     VSNFTPSQFALLHRRFPLVTNQVECSPLHVAPFYDGTFDQAQDLGVAPMIWSALAGGR
                     LFAGEDGDARRVRAVLERLAAERGIAVTTLVYAWLLRHPARLLPLTGSRRLEAMREAV
                     AALEVELDAQAWYEIWVAGNGAPAP"
     gene            4222..4737
                     /locus_tag="VF453_14270"
     CDS             4222..4737
                     /locus_tag="VF453_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEX7688875.1"
                     /translation="MTSQSSTSQRHGSAPKPGQQAGSRVAAADWDDEDTPPRPLTAQE
                     AEAFRGQRKAPSPWRVVLVQAEAGAVIALLLGLLLGLSAGLSALYGAATAIVPGALMA
                     RGATRMADSRSPMATALGLLGWESAKIVCSVAMLVSAVRVLHPVVWPALLVTLGGCLT
                     VYWVALAWRSR"
     gene            4768..5631
                     /gene="atpB"
                     /locus_tag="VF453_14275"
     CDS             4768..5631
                     /gene="atpB"
                     /locus_tag="VF453_14275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX7688876.1"
                     /translation="MAAEEHAPTAGEYIHHHLTHLSNIESDAPLNFHVVHWDSVFFSS
                     VLGLVFLLIGLVVARRVTSGVPGRLQAAMEFMIEMVDNQAKAIVHNATSRKMVAPLAL
                     TVFVWIFLLNAMDLLPVDLLPFLFQHVSGNPEAHLRVVPTADISVTMGMSVAVLLICL
                     YYNVKIKGLGGWGHELVVAPFGTSKNPIFAVLLGIVNFAMQMIEFVAKTVSHGMRLYG
                     NMYAGELIFLLIALLGGAFTFTAGGFGLALMHIFAGSVWAIFHILIITLQAFVFMMLT
                     LVYIGQAHDAH"
     gene            5722..5988
                     /gene="atpE"
                     /locus_tag="VF453_14280"
     CDS             5722..5988
                     /gene="atpE"
                     /locus_tag="VF453_14280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX7688877.1"
                     /translation="MELISYVALAAGIIIGLGACGACIGIGIMGSKYLESAARQPELM
                     GELQTKMFLLVGLIDASFIIGLGIALWFTTTNPFLAQLAQVAGH"
     gene            6076..6546
                     /locus_tag="VF453_14285"
     CDS             6076..6546
                     /locus_tag="VF453_14285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX7688878.1"
                     /translation="MSITATLIIQIIVLLILVGVTMKYIWPPITAALDERARKVAEGL
                     SAADKARTELANANQRVEQQLAAARDDAAKRLANAEHLALKIVEEAKTRASEEGAKIV
                     AQARAEAEQAAVKVREQLRDEVAALAVKGAEQILRHEVNPAVHAELLGRLKTEL"
     gene            6551..7084
                     /locus_tag="VF453_14290"
     CDS             6551..7084
                     /locus_tag="VF453_14290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX7688879.1"
                     /translation="MAEPSTIARPYAAALFQASTAADGGKLVAQLDALARIADDEGLR
                     QFASDPKVRGDQVFEVVSGIAGADLDAKLRNLLGTVIENGRLPVLPEIASQFRALVNE
                     RSGSADAHIESAFPLDAAQQAELVQTLEKRFGRQLKAQVTVDPSLIGGVRVTVGDEVL
                     DTSVRARLEQMRAALTH"
BASE COUNT         1031 a         2615 c         2588 g         1032 t
ORIGIN      
        1 gacgcccgcc tcgccgcgtt cgcgcgttgg acgaacctgt cggagacgac ctttctcctg
       61 ccgccgacct cgcccgaggc cgactaccgc gtgcgcatct tcacgcccgg cggcgagctg
      121 ccgttcgccg gccatccgac gctcggctcg tgccacgcct ggctggccgc cggcggacga
      181 ccgcggcgcg acgacgtcgt cgtgcaggaa tgcggcgtcg gcctggtgcc gatccgccgc
      241 ttgccccggc tcgcgttcgc ggccccgccc gccgccttcg agccggtgga cgcggcattg
      301 ctgcagcggg tgtgcgcggc gctcgggctc gacccggcgc gcgtgcgcga cgcgacctgg
      361 gccgacaacg ggccgcggca gctgacgctg ctggtcgacg ccgcggccac ggtgctcgcg
      421 ctgcgacccg accacgcggc gctcaagacg ctcgccaagg tcggggtcgt cggcccgcat
      481 ccggacggcg ccgaatgccg cttcgaggtg cgcttcttcg ccgccgcgat cggcgtgaac
      541 gaggatccgg tcaccggcag cctcaacgcc aagatcgcgc aatggctgat cggcagcggg
      601 cgcgcgccgg cgtcgtacgt cgccgcgcag ggcaccgcga tgggccgcgc cgggcgcgtg
      661 cacgtgcagc gcgatgccga cggcatgtgg atcggcggcg acgtcgcgcc ctgcgtctcg
      721 ggcacggtgc tattgtgagc gccctcgcgg ccgcttgcgg gcgcgccgcc gggtcgtcca
      781 acgtcgagga attcgcatga gcaggatcgt cgaatcaccg ctgcactacg gcgccgccgg
      841 ccgcgcctac cggggcctgc tgacggcgcg cgacgacggc acgcccgcca aggccgccgt
      901 cgtgctgctg ccggactggc gcgggcagag cgacctggcg cgcgaccatg cccgcgtgct
      961 ggtcgacgcg ggctgcgccg tcgccgtcgc cgacctctac ggcgacgggt tcagcccgac
     1021 cgacccggcg caggtcggcc cgatggtcaa gcggctgatg gagcatcgcg aggacggcgt
     1081 ggccgcgctg tcggcatgcg tcgaggcgct gagcgagcgc gtcggcgcgt cggtgccggt
     1141 gttttgcctg ggctacagcg ccggagggat ggtggcgctg gactacggac gcagcggcgc
     1201 gaaggtggcc ggcatcatcg tgtgctccgc attgctgaag accgcggctc cgggcatgtc
     1261 gacgcgcatc gccgcgccgg tgctgatgct gcagggcacg cgggacgagg tgtcgcccgt
     1321 ctcgacgatc gccgaggtca tcgcggagat ggacgccgcc ggcaaccgct tccgcatcca
     1381 tctgtacggc cagacccacc acgccttcga caacccgcag gcggggacgg acccgaccgc
     1441 gcggctggtc tattcgccgg agtcggcgcg gctcgcgcgg cgcacgatcg cggagttcgt
     1501 ggccgaacag gccggcgcgg ccggccgcag cacgtgagga tccgatgacc gaggaactgt
     1561 tccgcgacga tgcgacgctc gccgaatgcg aggcgcgcgt cctcgcagcc gacgcgcgcg
     1621 gcgtgcagct cgatcgcacc gtgttctatc cgcagggcgg cggccaggcg ggcgaccgcg
     1681 gcgagctcgt gctgcccgac ggccgcgccg tcgccgtcgt cgacacgagg aagggcgaag
     1741 cgccgggcga gatcgtgcac gtgctcgcga accccgagga cgccgccgcc cttgcgcccg
     1801 gccaggcggt gcgcgcgcgc atcgacgtcg cgcgccgccg cgcccacatg cgcttccaca
     1861 ccgccacgca cctgctgtgc gcgctggtgc cgcatccggt ggacggctgc tcgatcacgg
     1921 cggagtacgc gcggctcgac ttccacatga ccgacgcgct cgacaaggcc gcgctggagg
     1981 acggcctcgc gcgcctggtc gcggccgcgc atcccgtgag ccatcgctgg atcagcgacg
     2041 ccgagctcga cgcgaacccg cagctcgtgc gcagcatgag cgtgcagccc ccgcgcggca
     2101 ccggtcgcgt gcggctgctc gagatcgaag gcgtcgacct gcagccctgc ggcgggacgc
     2161 acgtcgccaa cacgtcggag atcggcgcca tcggcgtgcg caagatcgag aagaagtcgg
     2221 cgcagacccg gcgcgtggtc ctcgaattcc gctgaggggc ggggtgctac cctgcgcggc
     2281 atggaagcct tcgccatccg cccgttctcg cgcgacgacg actcgctcgt cgacctgacg
     2341 gccctgctgc accgcgccta tgccccgctc ggcgcgatgg gcctgaacta caccgcggtc
     2401 gaccagccgc ccgagaagac cgcccagcgc ctgggctcgg gcacgtgctt cgtcgcgacc
     2461 gacggcgtcg cgctggtcgg caccatcctc gtccacccga cctacgacgc gaacgactgc
     2521 gactacttca cgcggccggg cgtcgcctgc gtgcaccagt tcgcggtcga gccttcgctg
     2581 cagggggccg gtgtcggccg tgcactgctg cggcatgccg aggactgggc gcgcgccgcg
     2641 ggcttccgcg aagtcgcgat ggacactgcc gaaccggcca cccatctcgt cgagttctac
     2701 gcccggcagg gctacgagcg cgtcggccac gtgcggtggc ccggcaaggt ctatcgaagc
     2761 gtcgtgctgg ccaagcgcct ggcggacgag ggcgtccggg ccgcctgacc ctcgtcgcgc
     2821 cgccgccccg gtcgctcaag gcgcgggcgc gccgttgccc gcgacccaga tctcgtacca
     2881 ggcctgggcg tcgagctcga cctccagggc cgcgacggcc tcgcgcatcg cctcgaggcg
     2941 gcgcgagccg gtcagcggca gcagtcgcgc gggatggcgc agcagccagg cgtagaccag
     3001 cgtcgtgacg gcgatgccgc gctcggcggc caggcgctcc agcacggcgc gcacgcggcg
     3061 ggcgtcgccg tcctcgccgg cgaacaggcg cccgcccgcg agcgccgacc agatcatcgg
     3121 cgcgacgccg aggtcctgcg cctggtcgaa ggtgccgtcg tagaacggcg cgacgtgcag
     3181 cggcgagcac tcgacctggt tggtgacgag cgggaagcgc cggtgcagca gcgcgaactg
     3241 cgacggcgtg aagttggaga cgccgaagtg cagcaccttg cccgcggcgc gcaggcgctc
     3301 gaagcaggcg gcgacctcgt cggcgtccat cagcgcgtcg gggcgatgga tcagcagcac
     3361 gtcgaggcgg tcggtgcgca ggttggccag ggaccggtcc accgacgcct cgatgtgcgc
     3421 ggcggaggtg tcgtagtggc cgatgcgatg cgccggccgc gccggcgaga ccatgcggat
     3481 gccgcacttg gtgacgatct gcaggcgatc gcgcaggccg ggcgcagccg ccagcgcctc
     3541 gccgaacagc gcctcgacgc ggtagccgcc gtagatgtcg gcgtggtcga aggtcgtgat
     3601 gccgaggtcg aggcagcctt cgatccagcg ccggcggccg gcgacatcga ggccccagtc
     3661 gtccatgcgc caggcgccgg cgacgatgcg cgagaaggcg gggccgccgg cggcggtgac
     3721 gagcgtggag gcgtggcgca tcgaggatct cgggaagcgg ggcggcgcgc gcggcgcggg
     3781 gtggacggcg attctcgcgc atgggcgagc gccgtcgcgc tcgggcgggt accggcgggt
     3841 tgcgcgcgga tcgccggcgg gcctgacgcc agcgagggcc tgccgatcgt caacgacggc
     3901 gggcctcgac ccgcgcagcg gcggctcgtg atccgctggc cgtgccgcga cgggctcgaa
     3961 ctcgcggccc ccgaagcctg acacgaggct ggcgtcccgc cgtgccgcag gggcgtcggt
     4021 ggtgcaccgg gtggccttgg cgcactcttt cgggttagaa tcgacaggct taattcaact
     4081 taggcggctt tttcggttgc atgcctccac ggcatgcccc gagggtgccg tcaaggtcct
     4141 tgcgtcggtc gaagcgctgg cgcgccgccg cttcccgcgc cttgcaaggg gggtgcccgg
     4201 tcagcaagct cctagatccc gatgacttcc cagagttcca cttcgcagcg acatggctcc
     4261 gcgccgaagc ccggccagca ggcaggctcg cgcgtcgccg ccgcggattg ggacgacgag
     4321 gacacgccgc cccgtccgct gacggcgcag gaggccgagg ccttccgcgg ccagcgcaag
     4381 gcgccgtcgc cctggcgcgt ggtgctggtg caggccgagg ccggtgccgt catcgcgctg
     4441 ctgctgggat tgctgctcgg gttgtcggcg ggcctgtcgg cgctgtacgg cgccgccacc
     4501 gcgatcgtgc cgggcgcgct gatggcgcgc ggcgcgacgc ggatggcgga cagccgctcg
     4561 ccgatggcga cggcgctcgg gctgctgggt tgggagtcgg cgaagatcgt gtgctcggtg
     4621 gcgatgctcg tcagcgccgt ccgggtgctg cacccggtgg tgtggccggc cctgctggtc
     4681 accctgggcg ggtgcctgac cgtgtattgg gtggcgctcg cgtggcgctc gcgttgacag
     4741 actagatcgg aaaactacgg acaccacatg gccgcagaag agcacgcacc gaccgccggt
     4801 gaatacatcc accaccacct gacccacctg tcgaacatcg agagcgacgc gccgctgaac
     4861 ttccacgtgg tccactggga ctccgtgttc ttctcgtcgg tgctgggcct ggtgttcctg
     4921 ctgatcggcc tcgtggtcgc gcgccgcgtg acctcgggcg tgcccggccg cctgcaggcc
     4981 gcgatggagt tcatgatcga gatggtggac aaccaggcca aggccatcgt ccacaacgcg
     5041 acgagccgca agatggtcgc cccgctggcg ctgaccgtct tcgtctggat cttcctgctc
     5101 aacgcgatgg atctgctgcc ggtcgacctg ctgccgttcc tgttccagca cgtctccggc
     5161 aatcccgagg cgcacctgcg cgtcgtgccg acggccgaca tctcggtcac gatgggcatg
     5221 tccgtcgccg tgctgctgat ctgcctgtac tacaacgtca agatcaaggg cctgggcggc
     5281 tgggggcacg agctggtcgt cgcgccgttc ggcaccagca agaacccgat cttcgccgtg
     5341 ctgctgggca tcgtgaactt cgcgatgcag atgatcgagt tcgtcgccaa gaccgtctcg
     5401 cacggcatgc ggctgtacgg caacatgtac gccggcgagc tgatcttcct gctgatcgcc
     5461 cttctgggcg gcgcgttcac gttcacggcg ggcggcttcg gcctcgcgct gatgcacatc
     5521 ttcgccggca gcgtgtgggc catcttccac atcctgatca tcacgctgca ggccttcgtg
     5581 ttcatgatgc tgaccctggt ctacatcgga caagcccacg acgcgcactg accgccgcgc
     5641 gctgccgggc gaccggcggc gcggcttgcg ccgttcgaac cacacccctt cccttcctct
     5701 catttctcaa ctggagtcaa catggaactc atcagctacg tcgccctggc cgccggcatc
     5761 atcattggtc tgggcgcctg cggcgcttgc atcggcatcg gcatcatggg cagcaagtac
     5821 ctcgagtccg ccgcccgcca gccggaactg atgggcgaac tgcagaccaa gatgttcctg
     5881 ctggtcggcc tgatcgacgc ctcgttcatc atcggcctgg gtatcgcgct gtggttcacc
     5941 acgacgaacc cgttcctggc ccagctggcg caggtcgccg gccactaagc cgccaggcac
     6001 ccagccgcgc gcgcgaccgc ttccgcgaga cggaagcggc gcgcgctccc cgctaacggc
     6061 tttcgaggca acaacgtgag cattaccgca accctgatca tccagatcat cgtgctcctg
     6121 atcctggtcg gggtcacgat gaagtacatc tggccgccca tcacggccgc cctggacgag
     6181 cgcgcgcgca aggtcgccga aggcctgtcg gccgccgaca aggcccgcac ggagctcgcc
     6241 aacgcgaacc agcgcgtcga gcagcagctc gccgccgccc gcgacgacgc cgccaagcgc
     6301 ctcgcgaacg cggagcacct cgcgctcaag atcgtcgagg aagccaagac gcgcgccagc
     6361 gaggaaggcg ccaagatcgt cgcccaggcc cgggccgaag ccgaacaggc cgcggtcaag
     6421 gtgcgcgagc agctgcgcga cgaggtcgcc gcgctggcgg tcaagggcgc cgagcagatc
     6481 ctgcgccacg aggtcaaccc ggcggtgcac gccgagctcc tcggtcgcct gaagaccgag
     6541 ctgtaagacc atggccgagc cctccaccat cgcccgcccg tacgcggcgg cgctgttcca
     6601 ggcgagcacc gccgcggacg gcggcaagct cgtggcgcag ctcgacgcgc tcgcgcgcat
     6661 cgccgacgac gaaggcctgc gccagttcgc ctcggacccg aaggttcgcg gcgaccaggt
     6721 gttcgaggtg gtctccggca tcgccggcgc cgacctcgac gccaagctgc gcaacctgct
     6781 cggcacggtg atcgagaacg gccgcctgcc ggtgctgccc gagatcgcgt cgcagttccg
     6841 cgccctggtc aacgagcgca gcggcagcgc cgacgcgcac atcgaaagcg cgttcccgct
     6901 cgacgccgcg cagcaggccg agctcgtgca gacgctcgag aagcgcttcg gccgccagct
     6961 caaggcccag gtcacggtcg acccgtcgct gatcggcggc gtgcgcgtga cggtcggcga
     7021 cgaggtgctc gacacctcgg tgcgcgcccg cctcgagcag atgcgcgccg cgctgacgca
     7081 ctgacgatcg aaccgccaca cagccacgcc catcaaccca ggcggccttg ccggcctcac
     7141 ctttgggagt taagtcatgc aattgaatcc cgctgaaatt tccgaactga tcaagagccg
     7201 catcgaaggc ctgggggcgt cgtccgacat ccgcaaccag ggcacggtcg tctcggtcac
     7261 cgacgg
//
LOCUS       DASVLN010000594         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_246070, whole genome shotgun sequence.
ACCESSION   DASVLN010000594 DASVLN010000000
VERSION     DASVLN010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246070"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VF453_14295"
     CDS             complement(<1..869)
                     /locus_tag="VF453_14295"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7688880.1"
                     /translation="MLSPKALMRSALTTGRESVTVFLSDVGHGLLDVSHSMLAMVGLA
                     VVAGALFMAGQDDLRDSLEARAWGWLEARHLARSGGEDLAAAAEQDPPQAGSGPANSS
                     STADLADLTRAQANVARWIVRRYHVAPEPIARLVQESWNVGQRDKIDPTLILAVMAVE
                     SSFNPFAQSPVGAQGLMQVMTKVHNDKYEAYGGHLAAFDPVSNLRVGVLVLKECISRA
                     GGLQGGLHFYVGAGTSDDDGGYAAKVLAEEELLKRVAAGQNVPTTAPIMAAVAPTPAA
                     AAAPVAQPVATPAP"
     gene            1132..2712
                     /locus_tag="VF453_14300"
     CDS             1132..2712
                     /locus_tag="VF453_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX7688881.1"
                     /translation="MKYRDLRDFMAGLEDSGELRRVRESVSPNLEMTALADRVLRAGG
                     PALLFERATGFDVPVLANLFGTPRRVALGMGAQDVSELRGIGQLLASLREPEPPKSLK
                     DAGHLWTMAKALWDMKPAVVRSGPCQDVVQAAGEIDLARWPIQTCWPGDVAPLVTWGL
                     VVTRGPRGVPKPRLRQNLGIYRQQVIGRRELIMRWLAHRGGALDFRDFARVNPGKPFP
                     IACVLGADPATTLGAVTPVPDALSEYQFAGLLRGGRTELVETQAGEDGLMLQVPARAE
                     VVLEGHIPPAEPGFTGTSEHGIPLREQGGYLHALEGPYGDHTGYYNERDWFPVFRVDR
                     LTHRRDPVYHSTYTGKPPDEPAVLGVALNEVFVPLLQKQFPEIVDFYLPPEGCSYRMA
                     VISMRKAYPGHAKRLMFGLWSVLRQFMYTKFIVVVDDDVDIRDWKEVIWAITTRMDPA
                     RDTTLVENTPIDYLDFASPVSGLGGKMGLDATSKWPGETQREWGRPITMAGALDAAAQ
                     ARVEAVFDAVMGIGRAGA"
     gene            complement(2952..>3421)
                     /locus_tag="VF453_14305"
     CDS             complement(2952..>3421)
                     /locus_tag="VF453_14305"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX7688882.1"
                     /translation="LRYGVWLSRETGIPLAASGGRGWAATGDGTPEAEVMARAAQAEF
                     GHPLRWVEPRSRDTRENAANTVALLRPAGVRTIVVVTSGWHMPRALRDFRRAADDPTL
                     GGGPGAPVLRLVAAPMGQAYPDDRAVLAWMPSGEGALRMRHVLRELLARRAGA"
BASE COUNT          476 a         1214 c         1237 g          494 t
ORIGIN      
        1 ggcgcgggcg tggcgacggg ctgcgcgacc ggtgccgcgg cggcggccgg cgtcggggcg
       61 acggcggcca tgatcggcgc ggtcgtcggc acgttctggc cggcggccac gcgcttgagc
      121 agttcttcct cggccaagac cttggccgca tagccgccat cgtcgtcgct cgtgccggcg
      181 ccgacgtaga agtgcagtcc gccctgaagg ccgcctgcgc ggctgatgca ttccttgagc
      241 accagcacgc ccacgcgcag gttgctcacg gggtcgaacg cggccaggtg gccgccgtag
      301 gcctcgtact tgtcgttgtg caccttggtc atgacctgca tcagcccctg cgcgcccacc
      361 gggctctgcg cgaacgggtt gaagctggac tccaccgcca tcacggccag gatcagcgtc
      421 gggtcgatct tgtcgcgctg gccgacgttc caggactcct gcaccaggcg ggcgatcggc
      481 tcgggggcga cgtggtagcg gcgaacgatc cagcgcgcga cattggcctg cgcgcgcgtc
      541 aggtcggcca ggtcggccgt cgacgacgag ttggcggggc cgctgccggc ctgcggcggg
      601 tcctgctcgg ccgcggcggc gaggtcctcg ccgccgctgc gcgccaggtg gcgggcttcc
      661 agccaacccc aggcgcgggc ctcgaggctg tcgcgcaggt cgtcctggcc ggccatgaac
      721 agggcgccgg ccaccacggc caggcccacc atggccagca tgctgtggct cacatccagc
      781 agcccatgcc cgacgtccga caggaagacg gtcaccgact cgcgcccggt cgtgagcgca
      841 gagcgcatca gtgccttggg gctaagcatc tctctccttt cagcagcagc ggcggctggc
      901 cggcgtggcg cgcagtgggc gctcgacgca tcgggaagcg gcgctggcga taatggctcg
      961 ctgccagtca tggccggtcg ggtctgcacc tgggcagccg gcggcggcga gtcagggtcg
     1021 ggccggctgc gggggtgccg ggataaccct ggggatcggt ctgggcggga ttgtggtcag
     1081 ctcgaaataa cccgtcaacc atatggactt tgaaaatcat aaccaaaaga tatgaaatac
     1141 cgtgatctgc gcgacttcat ggccggtttg gaggattcgg gcgagttgcg gcgtgtccgg
     1201 gagagcgtct cccctaatct ggagatgacc gccctggccg accgcgtgct gcgagccggc
     1261 ggcccggcgc tgctgttcga gcgcgcgacc ggcttcgacg tcccggtgct cgccaacctg
     1321 ttcgggacgc cgcgccgcgt cgcgctgggc atgggcgcgc aggacgtgtc cgagctgcgc
     1381 ggcatcggcc agctcctcgc gagcctgcgc gagccggagc cgccgaagtc gctcaaggac
     1441 gccggccacc tgtggacgat ggccaaggcg ttgtgggaca tgaagcccgc ggtcgtgcgt
     1501 tccgggccgt gccaggacgt cgtgcaggcg gccggcgaga tcgacctcgc gcgctggccc
     1561 atccagacct gctggcccgg cgacgtcgcg ccgctggtca cctggggcct ggtcgtgacg
     1621 cgcggcccgc gcggcgtgcc caagccgcgc ctgcgccaga acctgggcat ctaccgccag
     1681 caggtgatcg ggcggcgcga gctgatcatg cgctggctcg cgcatcgcgg cggggcgttg
     1741 gacttccgcg acttcgcgcg cgtgaacccg ggcaagccgt tcccgatcgc ctgcgtgctc
     1801 ggagccgacc cggcgaccac gctgggcgcc gtgaccccgg tgccggacgc cctgagcgag
     1861 taccagttcg ccggcctgct gcgcggcggc cgcacggagc tcgtcgagac gcaggcgggc
     1921 gaggacggcc tgatgctgca ggtgcccgca cgcgccgagg tcgtgctcga gggccacatc
     1981 ccacccgccg agcccggctt cacgggcacc agcgagcacg gcatcccgct gcgcgagcag
     2041 ggcggctatc tgcacgcgct cgagggcccg tacggcgacc acaccggcta ctacaacgag
     2101 cgcgactggt tcccggtatt ccgcgtcgac cgcctcacgc accggcgcga ccccgtctac
     2161 cactcgacct acaccggcaa gccgcccgac gagcccgcag tgctgggcgt cgcgctcaac
     2221 gaggtcttcg tgccgctgct gcagaagcag tttcccgaga tcgtcgactt ctacctgccg
     2281 cccgagggat gcagctaccg catggccgtc atctcgatga ggaaggccta cccgggccac
     2341 gccaagcggc tgatgttcgg cctgtggagc gtgctgcgac agttcatgta caccaagttc
     2401 atcgtcgtgg tggacgacga cgtcgacatc cgcgactgga aggaggtgat ctgggcgatc
     2461 accacccgca tggatcccgc ccgcgacacg acgctcgtcg agaacacgcc gatcgactac
     2521 ctcgacttcg cctcgccggt cagcggcctg ggcggcaaga tgggcctgga cgcgacctcc
     2581 aagtggcccg gcgagacgca gcgcgagtgg ggtcgcccga tcacgatggc cggcgccctg
     2641 gatgcggccg cgcaggcgcg cgtcgaggcc gtcttcgatg cggtgatggg tatcggaagg
     2701 gccggcgcct agacctttcg ccgcccgccc gcaagggggc agcaggctat gggagcccgc
     2761 cgtcccttcg ggcatttttt cttgcggcgc ccggcgaacg gggctatctt ggaactgcct
     2821 cgacgaggca actccccaag cgcagtcgat gcgcaaccgg ggccccggac gttccgctcc
     2881 agagccccat tggatggcgg tcaaggccat ccggaagccc cggcaccttc cagtgtccgg
     2941 ggcttcgtcg ttcaggcgcc ggcccgccgc gcgagcagct cgcgcagcac gtggcgcatg
     3001 cgcagcgcgc cttcgcccga gggcatccaa gcgaggacgg cgcggtcgtc gggataggcc
     3061 tgccccatgg gcgccgcgac cagccgcagg accggcgcgc ccgggccgcc gcccagcgtc
     3121 gggtcgtcgg cggcacgccg gaagtcgcgc agtgcacggg gcatgtgcca cccgctggtc
     3181 acgaccacga tcgtgcgcac gccggccggc cgcagcaggg cgacggtgtt ggcggcgttc
     3241 tcgcgcgtgt cgcgcgagcg tggttcgacc cagcgcaggg gatggccgaa ttcggcctgc
     3301 gccgcccggg ccatcacctc ggcttccggc gtgccgtcgc cggtggcggc ccagccgcgg
     3361 ccgccggatg cggccagcgg gatgccggtc tcccggctga gccagacgcc gtagcgcagc
     3421 c
//
LOCUS       DASVLN010000595         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_462578, whole genome shotgun sequence.
ACCESSION   DASVLN010000595 DASVLN010000000
VERSION     DASVLN010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462578"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VF453_14310"
     CDS             complement(<1..437)
                     /locus_tag="VF453_14310"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEX7688883.1"
                     /translation="MTTDLLLALAHHALAFTLLALIAVEFALLHKSVAPAMVRTLASL
                     DAAYGATAGLLVVVGFGRVFKGAKGAEFFLHNPVFWAKLAFFLAVGLVSIAPTLRFLR
                     WKRAARADAGFTPTAAEVAATRRWLLAELALFVPIPLLAAAMAR"
     gene            633..1274
                     /locus_tag="VF453_14315"
     CDS             633..1274
                     /locus_tag="VF453_14315"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7688884.1"
                     /translation="MARPSKTPARRGYHHGDLKNALRAAAAALIARQGVDAVSLREIS
                     QAAGVSHTAAYRHYADKQALLADLAEDGFRRLAAINRRLAASTPGGPVEQLKACGRAY
                     VRFGLAEPHLLHLMFSQAIPDWSLHPGLKEASDELAATLAEVVAAGQEGGGLRPAPLG
                     ELTLTAWSLVHGLALLLVGRRIPVPEIDDAFVEHMSQACVELLVQGLAKPARR"
     gene            complement(1340..1546)
                     /locus_tag="VF453_14320"
     CDS             complement(1340..1546)
                     /locus_tag="VF453_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688885.1"
                     /translation="MLATVLLPANPENPSAAASAPPDAGYGEDATPGAGGSLPGFLVR
                     LGALLAAAVAVLAVAAGMLASIPG"
     gene            1685..>2165
                     /locus_tag="VF453_14325"
     CDS             1685..>2165
                     /locus_tag="VF453_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7688886.1"
                     /translation="MPSLPEGPRAAAGAVLPIRILLVDDDEAFRESVCAAVARDGAMT
                     LAAQAGSVALAREAIVRGNFDVALVDLGLPDGNGIDLIREIARTRPETDVMVVTVFGD
                     EAHVLASVEAGATGYVLKRSLNDLGETVRELRAGGSPISPVIARQLLQRFKHGGGEAP
                     "
BASE COUNT          304 a          788 c          808 g          265 t
ORIGIN      
        1 cgcgccatcg ccgccgccag cagcgggatc ggcacgaaca gcgccagctc ggcgagcagc
       61 cagcggcgcg tggcggcgac ctcggcggcc gtcggcgtga agccggcgtc ggcgcgggcg
      121 gcgcgcttcc agcgcaggaa gcgcagcgtc ggcgcgatcg agacgaggcc caccgcgagg
      181 aagaacgcga gcttggccca gaacaccggg ttgtgcagga agaactccgc gcccttggcg
      241 cccttgaaga cacggccgaa gcccacgacg accagcagcc cggcagtcgc cccgtaggcc
      301 gcgtccagcg aggcgagggt gcgcaccatc gccggtgcga cggacttgtg cagcagcgcg
      361 aactcgacgg cgatcagcgc gagcagcgtg aaggcgagcg cgtggtgggc gagggcgagc
      421 agcaggtcgg tcgtcacggt cgtctccggt ctcggtgggt ggaatgtttc cactgggtac
      481 atcgatgcgt ggatgctacg gatcgatgtg accgatgtca acatggacgt gatcgccacg
      541 tcggcggcgc gcctgcgcga gcgatgacgc acccgtgcga aaatgtcacc gatgtcaaca
      601 tcccggccac ggccggcgct ccgatccccg cgatggcccg accgtcgaag acccccgcac
      661 gccgcggcta ccaccacggc gacctgaaga acgcgctgcg cgccgccgcg gcggcgttga
      721 tcgctcgcca gggcgtcgac gcggtgagcc tgcgcgagat ctcgcaggcc gccggcgtca
      781 gccacaccgc ggcctaccgc cactacgccg acaagcaggc gctgctggcc gacctcgccg
      841 aggacggctt ccgccgcctg gccgcgatca accgccggct ggcggcctcg acgccgggcg
      901 ggccggtcga gcaactcaag gcctgcgggc gcgcctacgt gcgcttcgga ctcgccgagc
      961 cgcacctgtt gcacctgatg ttcagccagg cgatcccgga ctggtcgctg catcccggcc
     1021 tgaaggaggc gagcgacgag ctcgccgcga cgctggccga ggtcgtcgcc gcgggccagg
     1081 aaggcggcgg cctgcgcccg gcgccgctgg gcgagctcac gctgacggcc tggtcgctgg
     1141 tgcacggcct cgcgctgctg ctggtcggcc ggcgcatccc ggtgccggag atcgacgacg
     1201 ccttcgtgga gcacatgtcg caggcctgcg tggagctgct cgtgcagggg ctcgcgaagc
     1261 cggcgcggcg ctgacatcct gcgcgcgatc ggccgtcccg ggaggcgggg ccgcgcgttg
     1321 cgcgggaggt cgcgaacggt cagccgggga tggacgccag catccccgcg gcgacggcga
     1381 gcacggccac ggcagcggcc agcagtgcgc ccaggcgtac caggaagccg ggcagcgagc
     1441 cgccggcccc cggcgtcgca tcctcgccgt accccgcgtc cggtggcgcg gacgccgccg
     1501 ccgatggatt ctcggggttc gcaggcagca ggacggtcgc gagcatggtg cttggggatg
     1561 gcgcgcggcg ccggggcgtg atccgatgcg acatcttgcg agcgggcggc gcgctccgga
     1621 ataccgcgat ccggggatgc gcccccgcgt ttacagcccg ccccgcgctt gctccaatcg
     1681 cccgatgccg tccctccccg aaggcccgcg cgccgccgcc ggcgccgtcc tgccgatccg
     1741 catcctgctc gtcgacgacg acgaggcgtt ccgcgaatcg gtctgcgccg ccgtcgcccg
     1801 cgacggcgcg atgacgctcg ccgcgcaggc gggctcggtc gcgctcgcgc gcgaggcgat
     1861 cgtgcgcggc aacttcgatg tcgcgctcgt cgacctggga ttgcccgacg gcaacggcat
     1921 cgacctgatc cgcgagatcg cgcgcacccg gcccgagacc gacgtgatgg tggtcaccgt
     1981 gttcggcgac gaggcgcacg tgctcgcctc cgtcgaggcc ggtgccacgg gctacgtgct
     2041 caagcgcagc ctgaacgacc tcggggagac ggtgcgggag ctgcgcgccg gcggctcgcc
     2101 gatcagcccc gtgatcgcgc gccagctgct gcagcgcttc aagcacggcg gcggggaggc
     2161 gcccg
//
LOCUS       DASVLN010000596         4268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_141076, whole genome shotgun sequence.
ACCESSION   DASVLN010000596 DASVLN010000000
VERSION     DASVLN010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141076"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VF453_14330"
     CDS             complement(<1..516)
                     /locus_tag="VF453_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7688887.1"
                     /translation="MSDAANTTPGAGAVVLQATQLGRRFTEGPLDVQVLSAVDLTVRA
                     GETLAVVGASGSGKSTLLHLLGGLDAPTGGRVVLMGQDVHALSPAAQGAFRNRHLGFV
                     YQFHHLLPEFSALDNVAMPLLIRREKAPEAHARALEMLDAVGLRARAEHRPAELSGGE
                     RQRVAIARALVT"
     gene            complement(509..1810)
                     /locus_tag="VF453_14335"
     CDS             complement(509..1810)
                     /locus_tag="VF453_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX7688888.1"
                     /translation="MRRLPARSRVGGESSVPFEWLIGWRYTRAGRAGRRNAFISFISA
                     FSMGGIALGVAALIIVLSVMNGFQKEVRDRMLAAIAHIQLTEQDGSAVADWRTLVAQA
                     ARIEPGVRGAAPYVETQSLLAHGDGMQGAIVRGIDPAEEPKVTAVSAELAQEGVLAKL
                     VPGSWNIVLGSELARALGVGVGDQVTVIAPGAQVTPAGALPRLKSFNVVGIFSVGHYE
                     YDSGLALANVDDAARLYRTGGPTGVQLRLADVNDARNAAARLAHGLDPGIRVTDWTRT
                     NAAWYDAVQIEKTMMFIILTLIVAVAAFNLVTTLVMTVTDKQADIAILRTLGASPRSI
                     MAIFMVQGASAGIAGTVGGVVFGLLVAFNIPSIVPFIEHLLHTTFLPASVYLIDHMPS
                     DPQAGDIVPICFWSLLFAFVATVYPSWRAANVRPAEALRYE"
     gene            1990..2922
                     /locus_tag="VF453_14340"
     CDS             1990..2922
                     /locus_tag="VF453_14340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7688889.1"
                     /translation="MKAPDFAGASREVLSFLHRRLGMEIWMVTRADDEDWVVLAADGH
                     GFGIEEGSAFPWTETFCSRMVSGEGPRVAPRVADVPAYDTLPIARRMPVGAYVGVPLF
                     DAGGDLLGTLCGLSAEPMPASLVDEQPMVEVLADMLSGLLNAELHTSALAREAENARA
                     EGLADPLTGLANARGWEAALAVEDERCRRYGATACVIAMELDGMISPAASPEARAAYD
                     AVLVRAAQALRQTVRRPDTVARLDGDRFVLMGVECTTAEAMALLRRLELSLQAAELSA
                     ALGMAMRHRSSDLLDTFERAVAAMEEHRDARARD"
     gene            3011..3490
                     /locus_tag="VF453_14345"
     CDS             3011..3490
                     /locus_tag="VF453_14345"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7688890.1"
                     /translation="MTDAFDAYRPPEARVDDDDLRPVAGRPLAGRGRRFAGLLLDMVG
                     VILFGMVVGFVIGVLQAFGVGPFLRDAIRGGMGERLFGWGLGLAYYLVFEGLWGRTPA
                     KMLLGMRVVGEDGGRPSFLRILGRTAARFIPLEAFTFLAGVPGLHDRVSKTRVVTVR"
     gene            3540..>4268
                     /locus_tag="VF453_14350"
     CDS             3540..>4268
                     /locus_tag="VF453_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688891.1"
                     /translation="MHLVIPFASALSEAAAQAAGTLALPNLERLVSRWAAEDADPIAA
                     DALPDDGPEYTLSLPHERVLAQARGWPVADGLLAFAAEAAREDGLDVEPGSGWGRVTP
                     AHWHVGRDEIHLLDPAGLELTQDEAATLHAALAPLFEELGWRWLRAASGRWYASHPSL
                     AALPTASIDRVIGRNVDLWLNDDPGALLVRRLQSEVQMMLYVHPLNDAREARGAQPVN
                     SFWLAGTGAARPAAEAPPADLVVDE"
BASE COUNT          556 a         1556 c         1578 g          578 t
ORIGIN      
        1 cgtgaccagc gcgcgcgcga tcgcgacccg ctggcgctcc ccgcccgaca gctccgccgg
       61 ccggtgctcc gcgcgcgcgc gcaggccgac ggcgtccagc atctccaggg cgcgggcgtg
      121 ggcctcgggc gccttctcgc gccggatcag cagcggcatc gcgacgttgt ccagcgccga
      181 gaattccggc agcaggtggt ggaactggta gacgaagccg aggtggcggt tgcggaacgc
      241 gccctgcgcc gccggcgaca gcgcgtgcac gtcctggccc atcaggacga cgcggccgcc
      301 ggtgggggcg tccaggccgc cgagcagatg cagcagcgtg ctcttgcccg agcccgaggc
      361 gccgaccacg gccagcgtct cgccggcgcg caccgtcagg tcgacggcgg acagcacctg
      421 cacgtccagc ggcccctcgg tgaaccggcg gcccagctgc gtggcttgca gcacgaccgc
      481 gccggcgccc ggcgtcgtgt tcgcggcgtc actcatagcg cagcgcctcc gctggccgga
      541 cgttggcggc gcgccagctc gggtagacgg tcgcgacgaa ggcgaacagc agcgaccaga
      601 agcagatcgg cacgatgtcg ccggcctgcg ggtcgctggg catgtggtcg atcaggtaga
      661 cgctcgccgg caggaaggtg gtgtgcagca ggtgctcgat gaagggcacg atgctcggga
      721 tgttgaacgc caccagcagc ccgaagacga cgccgccgac ggtgcccgcg atgccggccg
      781 acgcgccctg caccatgaag atcgccatga tggagcgcgg gctcgcgccg agcgtgcgca
      841 ggatcgcgat gtcggcctgc ttgtccgtga ccgtcatgac gagcgtcgtg acgaggttga
      901 acgccgccac cgcgacgatc agcgtgagga tgatgaacat catcgtcttc tcgatctgca
      961 ccgcgtcgta ccaggcggcg ttggtgcggg tccagtcggt gacgcggatg cccgggtcga
     1021 ggccgtgcgc gaggcgggcg gcggcgttgc gggcgtcgtt gacgtcggcc aggcgcagct
     1081 gcacgccggt ggggccgccg gtgcggtaga ggcgcgcggc gtcgtccacg ttggcgagcg
     1141 cgaggccgct gtcgtactcg tagtggccga cggaaaagat gccgaccacg ttgaacgact
     1201 tcagccgcgg caacgcgccg gcgggcgtga cctgcgcgcc cggggcgatg acggtgacct
     1261 ggtcgccgac gccgaccccc agcgcgcgcg cgagctcgga gccgaggacg atgttccagc
     1321 tgccggggac gagcttggcg agcacgcctt cctgcgcgag ctccgccgac acggcggtga
     1381 ccttgggttc ctccgccggg tcgatgccgc gcacgatcgc gccctgcatg ccgtcgccgt
     1441 gggcgagcag ggactgcgtc tcgacgtagg gcgcagcgcc gcgcacgccg ggctcgatgc
     1501 gcgcggcctg cgccacgagc gtgcgccagt cggccacggc gctgccgtcc tgctcggtca
     1561 gctggatgtg cgcgatcgcg gcgagcatgc ggtcgcgcac ctccttctgg aagccgttca
     1621 tcaccgacag cacgatgatc agcgcggcca cgccgagcgc gatgcctccc atcgagaacg
     1681 cggagatgaa cgagatgaag gcgttgcgcc gccccgcgcg gccggcgcgc gtgtagcgcc
     1741 agccaatcaa ccattcgaaa ggcaccgagg attccccgcc cacgcgggag cgcgcgggca
     1801 accggcgcat tctagacagg tgcccggcgg gtgctccggg cggatccggt tccaacgccg
     1861 cgcgtcgcga ccgcgcgaaa cacgcgtaat tgaccgtttt tggacgcccc ggcggcgcgc
     1921 gcttgcgtaa gatcgacggg atcgactggc gccgcccttc ctcgacggcg catgaggaac
     1981 gaacgagaga tgaaagcacc cgacttcgcg ggcgcctcgc gggaggtgtt gtccttcctg
     2041 caccggcgcc tggggatgga gatctggatg gtcacgcgcg ccgacgacga ggactgggtg
     2101 gtcctcgccg ccgatggcca cggcttcggc atcgaggaag gcagcgcctt cccgtggacc
     2161 gagaccttct gctcccgcat ggtcagcgga gagggcccgc gcgtcgcgcc gcgcgtcgcc
     2221 gacgtcccgg cctatgacac cctgcccatc gcgcgccgga tgcccgtggg cgcctacgtc
     2281 ggcgtgccgc tgttcgacgc cggcggcgac ctgctgggca cgctgtgcgg gctcagcgcc
     2341 gagccgatgc cggcctcgct cgtcgacgag cagcccatgg tcgaggtcct tgccgacatg
     2401 ctcagcggcc tgctcaacgc cgagctgcat accagcgcgc tcgcacgcga ggctgagaac
     2461 gcgcgcgccg agggcctggc cgacccgctg accggcctcg ccaacgcgcg cggctgggag
     2521 gccgcgctcg ccgtcgagga cgagcgctgc cgccgctacg gcgccaccgc ctgcgtcatc
     2581 gcgatggagc tcgacggcat gatctcgccc gcggcttcgc ccgaggcgcg cgcggcctac
     2641 gacgccgtgc tggtgcgcgc cgcccaggcg ctgcgccaga ccgtgcgtcg ccccgacacc
     2701 gtcgcccgcc tggacggcga ccgcttcgtg ctgatgggcg tcgagtgcac caccgccgag
     2761 gcgatggccc tgctgcgccg cctcgagctg agcctgcagg ctgccgagct gtcggcggcc
     2821 ctgggcatgg cgatgcgcca ccgcagcagc gacctgctcg acaccttcga gcgcgccgtc
     2881 gccgcgatgg aggagcaccg ggacgcgcgg gcgcgcgact gaacgcgcga tcccgggcgc
     2941 gtccgggtcg cccctggtgg gtccgcgcgc cgagggcgtc gacaattcct cctcgacagg
     3001 gaggagaccc atgaccgacg ctttcgatgc ctaccgccca cccgaggcgc gcgtggacga
     3061 cgacgacctt cgcccggtgg cgggtcgccc gctcgccggc cggggccgtc gcttcgccgg
     3121 cctgctcctg gacatggtcg gggtgatcct gttcggcatg gtcgtgggct tcgtcatcgg
     3181 cgtcctgcag gcgttcggcg tcggcccgtt cctgcgcgac gccatccggg gcgggatggg
     3241 cgagcgcctg ttcggctggg ggctgggctt ggcctactac ctggtcttcg aaggtctctg
     3301 gggtcgcacg ccggccaaga tgctgctggg catgcgcgtg gtcggcgagg acggcggccg
     3361 gccgagcttc ctgcgcatcc tcgggcgcac cgcggcccgc ttcatcccgc tcgaggcgtt
     3421 caccttcctc gcgggcgtgc ccgggctgca cgatcgggtg tcgaagacgc gcgtggtgac
     3481 ggtccgctga aggcacccgg aggcgcggcc gcgccggccg tgcgcgggat aatcatccga
     3541 tgcacctcgt gatccccttc gcctcggcgc tgtccgaggc cgcggcgcag gccgccggca
     3601 ccctggccct gccgaacctg gagcgcctcg tctcgcgctg ggccgccgag gacgccgacc
     3661 cgatcgccgc ggacgcgctg ccggacgacg gccccgagta cacgctgtcg ctgccgcacg
     3721 agcgcgtcct cgcgcaggcg cgcggctggc cggtcgccga tggcctgctg gcgttcgccg
     3781 ccgaggccgc ccgcgaggac ggcctggacg tcgagcccgg cagcggctgg ggccgcgtga
     3841 ccccggcgca ctggcacgtc ggccgcgacg agatccacct cctcgacccg gccgggctcg
     3901 agctcacgca ggacgaggcc gccacgctgc acgccgcgct cgcgccgctg ttcgaggaac
     3961 tcggctggcg ctggctgcgc gcagcctcgg gccgctggta cgcgagccat ccgtcgctcg
     4021 ccgcgttgcc caccgcgtcg atcgaccgcg tgatcgggcg caacgtcgat ctctggctca
     4081 acgacgaccc gggcgccctg ctcgtgcggc ggctccagag cgaggtgcag atgatgctgt
     4141 acgtgcaccc gctcaacgac gcccgcgagg cgcgcggcgc gcagcccgtc aactcgttct
     4201 ggctggccgg cacgggcgcg gcgcgtccgg ccgccgaggc gccgccggcc gacctcgtgg
     4261 tcgacgag
//
LOCUS       DASVLN010000597         6563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_517060, whole genome shotgun sequence.
ACCESSION   DASVLN010000597 DASVLN010000000
VERSION     DASVLN010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517060"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VF453_14355"
     CDS             <1..473
                     /locus_tag="VF453_14355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEX7688892.1"
                     /translation="PLFGLRADDVMPGAPVQTVSTGTPQLMMLLRDHDALRRARPPED
                     PHYSRYKAGSDFFSPHLFCVGGATPDGDTFARHFGIAPDLPEDPVTGSATGGMAAYLW
                     RYGHLARPRFVAEQGHWMGRPGRIDVTLAGPRDAIEHVQVAGTAVVLVEGVLRD"
     gene            complement(502..822)
                     /locus_tag="VF453_14360"
     CDS             complement(502..822)
                     /locus_tag="VF453_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX7688893.1"
                     /translation="MTAPDPAACLSVRDGRVVLSLSVVPNARRTQVDGLHDGALRVRL
                     AAPPIEGRANDALVAWLAKSLGVARRDVELLRGEASRRKQVAIDVPLDAARAWLAGAL
                     RAGA"
     gene            complement(920..1519)
                     /locus_tag="VF453_14365"
     CDS             complement(920..1519)
                     /locus_tag="VF453_14365"
                     /inference="COORDINATES: protein motif:HMM:NF038053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like DNA-binding protein"
                     /protein_id="HEX7688894.1"
                     /translation="MNPGLLEHPGVFRNLIKEIEMATAKKPVSKKSAPAKKAAPAKKA
                     AAKKAAPAKKAPAKKAAPAKKAAPAKKAVAAKKAAPAKKAAAKKAAPAKKVAAKKAAP
                     AKKAAPAKKAAAKKAPAKKAAAKAPAKKAAAKAPAKKAAAKAPAKKAAAKAPAKKAAA
                     KKAAAPAAAPSAAAAAPAPAAKTALSPAAAWPFPTGNKP"
     gene            complement(1688..2947)
                     /locus_tag="VF453_14370"
     CDS             complement(1688..2947)
                     /locus_tag="VF453_14370"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEX7688895.1"
                     /translation="MLVWEDDLRSPQSSPPNGATAIDRDGRTASPSLREVQSAPLLTQ
                     AVDARAVPHSEPQVSPATSAHTQSTPQPGASHQARSTRRVTVSEKRIINGQTDVNQLV
                     PFKYKWAWEKYLATCANHWMPQEVNMSRDIATWKDPNGLTEDERRIIKRNLGFFVTAD
                     SLAANNIVLGTYRHITAPECRQFLLRQAFEEAIHTHAYQYIVESLGLDESEIFNAYHE
                     VASIRDKDEFLIPFIEAIMDPNFHTGTPQADRTLLRSLIVFACLMEGLFFYVGFTQIL
                     ALGRQNKMTGAAEQYQYILRDESMHCNFGIDLINQVKLENPHLWTTEFKDEIKGLFLK
                     AVDLEYRYAEDTMPRGVLGLNASMFKGYLRYIANRRAQQIGLEALFPNEENPFPWMSE
                     MIDLKKERNFFETRVIEYQSGGALSWD"
     gene            complement(3195..6116)
                     /locus_tag="VF453_14375"
     CDS             complement(3195..6116)
                     /locus_tag="VF453_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEX7688896.1"
                     /translation="MQGIASAINESGAAARPVSSGNTVAGSQSAYQGYQILRRNGAVV
                     AFEPSKIAVALMKAFLAVHGTQGAASASVRETVDGLTETVVRALLRSRPAGGTFHIED
                     IQDQVELGLMRGGHHEVARAYVLYRERRAQDRVKAGIVEAKVAEPELFVTDKGGRVPL
                     DTAKLRALIESACFGLGDDVQAEPVFAETRRNLYDGVPMEEVHKAAILAARTLIEKEP
                     AYTRATARLLLHTIRREILGEEVTQEQMQQRYADYFPTFIKRGIKAELLDEKLAQYDL
                     AKLGAALDASRDLQFDYLGLQTLYDRYFLHDQDVRIEMPQAFFMRVAMGLALGEIDRE
                     TRAIEFYNVLSTFDFMSSTPTLFNSGTRRSQLSSCYLTTVADDLDGIYEALKENALLS
                     KFAGGLGNDWTNVRALGSYIKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYL
                     ESWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMRRVMEGGDWTLFSPSTCPDL
                     HDRIGADFEKAYVEYEAKADRGEIKLFKRMPAKDLWRRMLSMLFETGHPWITFKDACN
                     VRSPQQHVGVVHSSNLCTEITLNTSDTEIAVCNLGSVNLAQHLVETSDGLVIDHSKLR
                     KTVATAMRMLDNVIDINYYAVKKARDSNLRHRPVGLGIMGFQDCLYQLRVPYASQAAV
                     EFADRSMEAVCYYAYTASTELAAERGRYSSFRGSLWDKGILPPDSIDLLAKNRGGYVE
                     VDRSSTLDWDALRAKIAQHGMRNSNCVAIAPTATISNIIGVDASIEPCFGNLSVKSNL
                     SGEFTVVNEYLVRDLKRLGLWDSVMLMDLKHYNGSLRAVDRVPEELKALYATAFEVDA
                     TWLVEAGARRQKWIDQAQSLNIYLAGASGKKLDETYKLAWLRGLKTTYYLRTMGATHA
                     EKSTVDRKQLNAVPSSSHGGGLSALDAVAAQAQAMLAESGPATDIKFCGVDDPTCEAC
                     Q"
BASE COUNT          929 a         2157 c         2297 g         1180 t
ORIGIN      
        1 cgccgctgtt cggcctgcgc gccgacgacg tgatgccggg cgcgccggtg cagaccgtga
       61 gcaccggcac cccgcagctg atgatgctgc tgcgcgacca cgacgccctg cgccgcgcac
      121 ggccgcccga ggatcctcac tactcgcgct acaaggcggg ctcggacttc ttcagcccgc
      181 acctgttctg cgtcggcggc gcgacgcccg acggcgacac cttcgcccgg cacttcggca
      241 tagcgcctga cctgccggag gatccggtca ccggctccgc gaccggcggc atggccgcat
      301 acctctggcg gtatggccac ctggcgcggc cgcgcttcgt cgccgagcag ggccattgga
      361 tgggacgccc gggtcgcatc gacgtgacac tggccggacc acgggacgcg atcgagcacg
      421 tgcaggtcgc gggcacggcg gtggtgctgg tcgagggcgt gctgcgcgac tgaccggcgc
      481 atccgccgcg gcgcgcatcg ttcacgcgcc tgcgcgcagc gcgcccgcga gccacgcgcg
      541 cgcggcgtcg aggggcacgt cgatcgccac ctgcttgcgc cggctggcct cgccgcgcag
      601 cagctcgacg tcgcgtcggg ccactcccag cgacttcgcg agccaggcga cgagcgcgtc
      661 gttggcacgc ccttcgatcg gcggcgcggc caggcgcacg cgcagcgcgc cgtcgtgcag
      721 cccgtccacc tgcgtgcgcc tggcgttggg cacgacgctg agcgacagca cgacgcggcc
      781 gtcgcgcacc gagaggcagg ccgcaggatc gggagcggtc atgatcgcgc gatggcgcgg
      841 agtggatgcg acgcgctggg cacagacgga aaaaagcccg gcgcggggcc gggcttgccg
      901 ttcaggagaa cgcgtccgct cagggcttgt tgcccgtcgg gaagggccag gcggcggccg
      961 ggctcagcgc ggtcttcgca gcgggcgcag gcgcagcggc cgcagccgaa ggggccgcgg
     1021 cgggcgcggc ggccttcttc gcggcagcct tcttcgcagg ggccttggca gcggccttct
     1081 tcgcaggcgc cttggcggca gccttcttcg caggggcctt cgcggcagcc ttcttcgcag
     1141 gcgccttggc ggcggccttc ttcgcaggcg ccttcttggc agcggccttc ttcgccggag
     1201 ccgccttctt cgccggagcc gccttcttcg ccgcgacctt cttggccgga gcggccttct
     1261 tcgccgccgc cttcttggcc ggagccgcct tcttcgcagc gacggccttc ttcgccggag
     1321 ccgccttctt ggccggagcc gccttcttgg ccggggcctt cttcgccgga gccgccttct
     1381 tcgcggcggc cttcttggcc ggagcggcct tcttcgccgg ggcagacttc ttgctgaccg
     1441 gtttcttcgc agttgccatt tcaatctcct tgatcaagtt acggaaaaca ccgggatgct
     1501 cgagcaaccc gggattcatc gcctgctcgc ctttaccgtc gcttcagcga cggccggctc
     1561 gcatgacgat gaactcgttt gcgacatcgg cgaaccccat ctgacggagg tccttccgac
     1621 ggcgcgaatc ttgatcggtc acaacatctg gaaaccggtc gcgcccgctg tctcgcgggc
     1681 ctcgacatca gtcccaggac agggcgccac ccgattggta ctcgatgacg cgggtttcga
     1741 aaaagttgcg ttccttcttg aggtcgatca tctcgctcat ccaggggaac gggttttcct
     1801 cgttcgggaa gagcgcctcc aggccgatct gctgggctcg ccggttggcg atgtagcgca
     1861 ggtagccctt gaacatcgac gcgttcagac ccagcacgcc gcgcggcatg gtgtcttcgg
     1921 cgtagcggta ctcgaggtcg acggccttca ggaacaggcc cttgatctcg tccttgaact
     1981 cggtcgtcca caggtgcgga ttctcgagct tgacctggtt gatgaggtcg atgccgaagt
     2041 tgcagtgcat cgactcgtcg cgcaggatgt actggtactg ctcggcggcg cccgtcatct
     2101 tgttctggcg gcccagcgcg aggatctgcg tgaagccgac gtagaagaac aggccttcca
     2161 tcaggcacgc gaagacgatc aggctgcgca gcagcgtgcg gtcggcctgc ggcgtgccag
     2221 tgtggaagtt cggatccatg atcgcctcga tgaaggggat caggaactcg tccttgtcgc
     2281 ggatcgaggc gacctcgtgg tacgcgttga agatctcgct ctcgtccagg cccagcgact
     2341 cgacgatgta ctggtaggcg tgcgtgtgga tcgcttcctc gaacgcctgg cgcagcagga
     2401 actggcggca ctcgggggcg gtgatgtggc ggtaggtgcc gagcacgatg ttgttcgccg
     2461 cgagcgagtc cgccgtgacg aagaagccga ggttgcgctt gatgatgcgg cgctcgtcct
     2521 cggtcaggcc gttcggatcc ttccacgtcg cgatgtcgcg cgacatgttg acctcctgcg
     2581 gcatccagtg gttcgcgcag gtggcgaggt acttctccca cgcccacttg tacttgaacg
     2641 gcaccagctg gttgacgtcg gtctggccgt tgatgatgcg cttctcggag accgtcacgc
     2701 ggcgcgtgct gcgcgcttgg tgcgacgcgc ccggctgcgg cgtcgactgc gtgtgcgccg
     2761 aggtggcggg cgagacctgc ggctccgaat gcggcaccgc gcgcgcatcg acggcttgcg
     2821 tcaggagggg agcggactgc acttcacgga gtgacgggga ggccgtgcgc ccgtcgcgat
     2881 cgatcgcggt ggcgccattc ggcggagaac tttgcggaga ccggaggtcg tcttcccaga
     2941 ccagcatgtg tgtgcttcct atggctgcga attatcggag tgttgtgtcg aaacaccaag
     3001 cacttattga cagcgcgccg cgctcgttgt tgcggtatcg catcgaagaa tcgacatcgg
     3061 catcacgcat cgacatgcgt tcctcgttgc atcgcgatgc atgtcgcgcg acgtcgcggt
     3121 ccgtgtcgat gcgcgctcgc ggcgagcgca tcgcacgcgc gtccgggacg cgtcgcgcga
     3181 tgcgcgccgt cgtcttactg gcaggcctcg caggtcggat cgtcgacgcc gcagaacttg
     3241 atgtcggtgg cgggtccgga ctccgccagc atcgcctgtg cctgcgccgc gacggcgtcg
     3301 agcgcggaca gcccgccgcc gtgcgacgag ctcggcaccg cgttgagctg cttgcggtcg
     3361 accgtgctct tctcggcgtg cgtcgcgccc atcgtgcgca ggtagtaggt cgtcttcagg
     3421 ccgcgcagcc aggcaagctt gtaggtctcg tcgagcttct tgcccgacgc gccggccagg
     3481 tagatgttga gcgactgcgc ctggtcgatc cacttctggc ggcgcgcgcc ggcctcgacc
     3541 agccacgtcg cgtcgacctc gaacgcggtg gcgtacaggg ccttcagctc ctcgggcacg
     3601 cggtcgacgg cgcgcagcga gccgttgtag tgcttcaggt ccatcagcat caccgagtcc
     3661 cacaggccca ggcgcttgag gtcgcgcacg aggtactcgt tgacgacggt gaactcgccc
     3721 gacaggttgg acttgaccga caggttgccg aagcacggct cgatcgacgc gtcgacgccg
     3781 atgatgttgg agatcgtcgc cgtcggcgcg atcgccacgc aattggaatt gcgcatgccg
     3841 tgctgggcga tcttcgcgcg cagcgcgtcc cagtcgagcg tggacgagcg gtcgacctcg
     3901 acgtagccgc cgcggttctt cgcgagcagg tcgatcgagt ccggcggcag gatgcccttg
     3961 tcccacagcg agccgcggaa cgaggaatag cggccgcgtt cggcggcgag ctcggtcgag
     4021 gccgtgtagg cgtagtagca gacggcttcc atcgagcggt cggcgaactc caccgcggcc
     4081 tggctcgcgt acggcacgcg cagctggtac aggcagtcct gaaagcccat gatgccgagt
     4141 ccgaccgggc ggtggcgcag gttcgagtcg cgcgccttct tgaccgcgta gtagttgatg
     4201 tcgatcacgt tgtcgagcat gcgcatcgcg gtggcgaccg tcttgcgcag cttcgagtgg
     4261 tcgatcacca gcccgtcgct cgtctcgacc aggtgctggg cgaggttgac cgagccgagg
     4321 ttgcagacgg cgatctccgt gtcgctcgtg ttgagcgtga tctccgtgca caggttggac
     4381 gagtgcacga cgccgacgtg ctgctgcggc gagcgcacgt tgcacgcgtc cttgaacgtg
     4441 atccagggat ggccggtctc gaacagcatc gagagcatgc ggcgccacag gtccttggcg
     4501 ggcatgcgct tgaacagctt gatctcgccg cggtcggcct tggcctcgta ctcgacgtag
     4561 gccttctcga agtcggcgcc gatgcggtcg tgcaggtcgg ggcaggtcga cggcgagaac
     4621 agcgtccagt cgccgccctc catcacgcgg cgcatgaaca ggtcggggat ccagttggcc
     4681 gtgttcatgt cgtgcgtgcg gcggcggtcg tcgccggtgt tcttgcgcag ctcgaggaac
     4741 tcctcgatgt cgaggtgcca cgactcgagg taggcgcaga ccgcgccctt gcgcttgccg
     4801 ccctggttga ccgcgacggc ggtgtcgttg acgaccttga ggaagggcac gacgccctgg
     4861 ctcttgccgt tggtgccctt gatgtaggag ccgagcgcgc ggacgttggt ccagtcgttg
     4921 cccaggccgc cggcgaactt cgacagcagc gcgttctcct tgagcgcctc gtagatgccg
     4981 tccaggtcgt cggccaccgt cgtcaggtag cagctggaca gctgcgagcg gcgcgtgccc
     5041 gagttgaaca gcgtcggcgt cgacgacatg aagtcgaagg tcgacagcac gttgtagaac
     5101 tcgatcgcgc gcgtctcgcg gtcgatctcg cccagcgcga ggcccatcgc gacgcgcatg
     5161 aagaacgcct gcggcatctc gatgcgcacg tcctggtcgt gcaggaagta gcggtcgtac
     5221 agcgtctgca ggccgaggta gtcgaactgc aggtcgcgcg aggcgtcgag cgccgcgccc
     5281 agcttggcca ggtcgtactg cgcgagcttc tcgtccagca gctccgcctt gatgccgcgc
     5341 ttgatgaagg tcgggaagta gtcggcgtag cgctgctgca tctgctcctg cgtcacttcc
     5401 tcgccgagga tctcgcggcg gatcgtgtgc agcagcaggc gcgcggtggc gcgggtgtag
     5461 gcgggctcct tctcgatcag cgtgcgcgcg gcgaggatgg cggccttgtg cacctcctcc
     5521 atcggcacgc cgtcgtacag gttgcggcgc gtctcggcga acaccggctc ggcctggacg
     5581 tcgtcaccga ggccgaagca cgccgactcg atcagcgcgc gcagcttggc ggtgtcgagc
     5641 ggcacgcgcc cgcccttgtc ggtgacgaac agctcgggct cggcgacctt ggcctcgacg
     5701 atgccggcct tcacgcggtc ctgcgcgcgg cgctcgcggt acaggacgta ggcgcgcgcg
     5761 acctcgtggt ggccgccgcg catcaggccg agctcgacct ggtcctggat gtcctcgatg
     5821 tggaacgtgc cgccggccgg gcgcgagcgc agcagcgcgc gcacgacggt ctcggtcagg
     5881 ccgtcgacgg tctcgcgcac gctcgccgac gccgcgccct gggtgccgtg caccgcgagg
     5941 aaggccttca tcagcgcgac cgcgatcttg ctgggttcga aggcgacgac cgcaccgttg
     6001 cggcgcagga tctggtagcc ctggtaggcg gattgcgagc cggccaccgt gttgccggag
     6061 gacacggggc gcgcggcggc gccggactcg ttgatggcgg atgcgattcc ttgcatgtcg
     6121 acccctcttg ctggaattca gttcggtgtt gatgtatgtg gaacacgcaa tgcgcggccc
     6181 cggctccttg cggaacctcg tggggacggc attgcgtgga cacccaccgg acccttcgcg
     6241 gggacgcttc ttcctggacg aggcgcggcc gtttcggctg ccacctggat caccggacgc
     6301 gtcgtggtga gtgacatgtg gtgtggatca gatgcgtgac tgtagcacga gtgggcacta
     6361 tatctggggt gccggagccc ttcaagcact acccctagcg tgacaaccct cttgacgaat
     6421 ccacgatgac ggtttcgcga aatcgtctgc gcacgacagc ggatcgtctt ctccgcgcgc
     6481 caacccgcat ggacacgagc ggcgggcggt gcaacccgca tgcctgcggg ccttgcgggc
     6541 cgccgcatgc gcgcaggccg cat
//
LOCUS       DASVLN010000598         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_192738, whole genome shotgun sequence.
ACCESSION   DASVLN010000598 DASVLN010000000
VERSION     DASVLN010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192738"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2380
                     /locus_tag="VF453_14380"
     CDS             <1..>2380
                     /locus_tag="VF453_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7688897.1"
                     /translation="GPGAVLARIPEDELRRRLEAQPELAAMDRFLAVTAEDLGADLIY
                     LLDDAGNTVASSSAGTPTSPVGQHVAEPAYLDQLLERGSTREFAIGRVTGIPGLYFSA
                     AIARNGRFAGAVVVKRNTPALAAMVGHEGAFVADAQGVVILAQDTKRLLLAMPGAAVA
                     AMDPATRESRYRRDRLPELRIAPWPGDPDGVLRTIGADPTPQVLVQRHMVFEALDVYV
                     AQPLPEIEALAHERLADFALLSVAGAGAIALVAAGLGGWRARRQVLDALDHLAAANHQ
                     LSVQMAERQRAEALLGESREVLEVHEARLRAVLRNLPEQVWMKDRAGAYLAVNAEFER
                     FQGRPAAEFLGRTDDDFESPERAARFAAQDRAALAAPEPLVFDDVDRPAAGGGDRRLQ
                     IIKSAVRDDRGQVIGVVGIARDMTEVLAANQALKTSEARLRATFETAGDAILIIRRAI
                     VVDCNSRAVQLLGARSRGELLGRRIDVLGPERQPGGESSRVLAARYVDAVRAGGQLTA
                     EWRCRRLDGGEFDAEITLSLFEHDGQRLLQSVLRDVSERKRMIAALESAKEEAERANR
                     SKSVFLANMSHEIRTPLNAVLGFTQLLLADRKLPEEAQSRLRVIHAAGNRLLGLINDV
                     LDLAKIESGGLQVALAPFDLLQELRDIDHLYGAHARAKGVALHSELTLAAPAVVMGDR
                     TKVGQIVSNLLGNALKFTERGRIGLHAWREAGAAERVWIEVEDTGVGIAEAELGELFV
                     PFRQGAAGQEKGGTGLGLVLSRDMARAMGGDLVVTSAPGVGTRVRVWLPLPPT"
BASE COUNT          311 a          862 c          906 g          301 t
ORIGIN      
        1 ggggccgggc gccgtgctcg cgaggatccc ggaagacgag ctgcgccgcc gcctggaggc
       61 ccagcccgag ctcgcggcga tggaccgctt cctggccgtg acggccgagg atctcggcgc
      121 cgacctgatc tacctgctgg acgacgccgg caacacggtc gcctccagca gcgccggcac
      181 gcccacgagc ccggtcggcc agcacgtcgc cgaaccggcg tacctcgacc agctgctgga
      241 gcgcggcagc acgcgcgagt tcgcgatcgg ccgcgtcacc ggcatccccg ggctgtactt
      301 ctcggccgcc atcgcgcgca atggccgctt cgccggggcg gtggtcgtca agcgcaatac
      361 gccggcgctg gcggcgatgg tgggccacga gggcgcgttc gtcgccgatg cgcagggcgt
      421 cgtgatcctc gcgcaggaca cgaagcggct gctgctcgcg atgccggggg cggcggtggc
      481 ggcgatggat ccggccacgc gcgagtcgcg ctaccgccgc gaccgcctgc cggagctgcg
      541 catcgcgccc tggccgggcg acccggacgg cgtgctgcgc acgatcggcg ccgacccgac
      601 gccccaggtg ctcgtgcagc ggcacatggt gttcgaggcg ctggacgtct acgtcgcgca
      661 gccgctgccc gagatcgagg ccctcgcgca cgagcgcctg gccgacttcg cgctgctgtc
      721 ggtggccggc gccggcgcga tcgcgctggt cgccgccggt ttgggcggct ggcgcgcgcg
      781 gcggcaggtg ctggacgcgc tggatcatct cgccgccgcc aaccaccagc tgtcggtgca
      841 gatggccgaa cgccagcgcg cggaggcact gctgggcgag tcgcgcgagg tgctcgaggt
      901 ccacgaggcg cgcctgcgcg cggtgctgcg caacctgccc gagcaggtgt ggatgaagga
      961 tcgcgcgggc gcctacctgg cggtcaacgc ggagttcgag cgcttccagg gccggcccgc
     1021 cgcggagttc ctgggccgca ccgacgacga cttcgagtcg cccgagcgcg ccgcgcgctt
     1081 cgccgcccag gaccgcgccg cgctcgccgc gccggagccg ctcgtcttcg acgacgtcga
     1141 ccgtcccgcc gcgggcggcg gcgaccgccg gctgcagatc atcaagagcg ccgtgcgcga
     1201 cgaccgcggc caggtcattg gcgtggtcgg catcgcgcgc gacatgaccg aggtgctggc
     1261 ggccaaccag gcgctgaaga cgagcgaggc gcgcctgcgc gcgaccttcg agaccgcggg
     1321 cgacgcgatc ctgatcatcc gccgcgcgat cgtcgtcgac tgcaactcgc gcgcggtgca
     1381 actgctgggc gcgcgctcgc gcggcgagct gctgggacgg cgcatcgacg tgctcgggcc
     1441 cgaacgccag ccggggggcg agtcctcgcg cgtgctcgcg gcgcgctacg tcgacgcggt
     1501 gcgcgcaggc ggccagctga cggccgagtg gcgctgccgg cgcctggacg gcggggagtt
     1561 cgacgccgag atcacgctca gcctgttcga gcacgacggc cagcgcctgc tgcagtcggt
     1621 gctgcgcgac gtcagcgagc gaaagcgcat gatcgcggcg ctcgagtcgg ccaaggagga
     1681 ggccgagcgc gccaaccgca gcaagagcgt gttcctcgcc aacatgagcc acgagatccg
     1741 cacgccgctg aacgccgtcc tcggcttcac gcagctgctg ctggccgatc gcaagctgcc
     1801 cgaggaggcg cagtcgcggc tgcgcgtgat ccacgccgcg ggcaaccggc tgctcgggct
     1861 gatcaacgac gtgctcgatc tcgcgaagat cgagtccggc gggctgcagg tggcgctcgc
     1921 gccgttcgac ctgctccagg agctgcgcga catcgaccac ctgtacggcg cccacgcgcg
     1981 cgccaagggc gtcgcgctgc acagcgaact cacgctcgcc gcgcctgcgg tcgtgatggg
     2041 cgaccgcacc aaggtcggcc agatcgtctc caacctgctg ggcaacgcgc tgaagttcac
     2101 ggagcggggc cgcatcgggc tgcatgcctg gcgggaggcc ggcgccgccg agcgcgtgtg
     2161 gatcgaggtc gaggatacgg gcgtcggtat cgcggaggcc gagctcggcg agctgttcgt
     2221 gcccttccgc cagggcgcgg ccggccagga gaagggcggc acgggcctcg ggctcgtgct
     2281 ctcgcgcgac atggcgcgcg cgatgggcgg cgacctcgtc gtcacgagcg cgcccggcgt
     2341 cggcacccgc gtgcgggtgt ggctgccgct gccgccgacc
//
LOCUS       DASVLN010000599         4270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_31660, whole genome shotgun sequence.
ACCESSION   DASVLN010000599 DASVLN010000000
VERSION     DASVLN010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31660"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..317)
                     /locus_tag="VF453_14385"
     CDS             complement(39..317)
                     /locus_tag="VF453_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688898.1"
                     /translation="MNNSSDKQGKIVVRKRKLDKEIIARAPSLERIAQTLSTEKQEQK
                     MKEPKPDIEEFDEWEGDLKSEVQHLTSGKVLRWKKEASTGNSGLKSAC"
     gene            complement(314..694)
                     /locus_tag="VF453_14390"
     CDS             complement(314..694)
                     /locus_tag="VF453_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688899.1"
                     /translation="MNSLDKQIEEAAPSLNCLTEWMRFILSEDRHYYRQGVSVQIFGE
                     GDSSYAVFERDVSSSPAAVDQYVLLASDPERKLFSLLCFLAESGFELASIMSRLEAAD
                     YSDENGARFKHVTATYSRVTEVKS"
     gene            complement(829..1440)
                     /gene="gstA"
                     /locus_tag="VF453_14395"
     CDS             complement(829..1440)
                     /gene="gstA"
                     /locus_tag="VF453_14395"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEX7688900.1"
                     /translation="MKLYYSPGACSLSPHIVLREAGIKFDLEKTALKTHTLVADGSDY
                     YAVAPKGQVPMLELDDGQRLTEGPVIVQYIADHAPASGLAPAAGTMERYRLMEWLNFV
                     TSELHKGFSPLFNAQMPQAAKEIFAARLKDKFAYVDKALAGREYLMGAQFTVADAYLY
                     TVATWADGLGLDLSGLADFAAWRARVTARPAVQAALVAEGLRK"
     gene            1769..2449
                     /gene="aqpZ"
                     /locus_tag="VF453_14400"
     CDS             1769..2449
                     /gene="aqpZ"
                     /locus_tag="VF453_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HEX7688901.1"
                     /translation="MKRHLAELIGTFWLVLGGCGSAVLAAAFPGLGIGLLGVSLAFGL
                     TVVTMAYAIGHVSGCHLNPAISIGLWAGRRFEGRQVLPYIVAQVLGATLAGAALYAVA
                     SGKAGFAAGGFASNGYALHSPGGYSLAAGMVCEVVMSAVFLFVIMGATDRRAPAGFAP
                     LAIGLTLTLIHLVSIPVTNTSVNPARSTGVALFADAWALQQLWAFWVAPIAGAVIGAL
                     AYRFVAED"
     gene            2500..2889
                     /locus_tag="VF453_14405"
     CDS             2500..2889
                     /locus_tag="VF453_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688902.1"
                     /translation="MDTQQLISEFLASEHGAQAVQALGAQGIGQADAQQLLTHATGAA
                     VDHVEEQHAGLMGEHPGKSFFAAFAAGLVRGDGFFKSLVDGGEGVITGRMAEAIAARA
                     GIDPATASTIAAAATPYVLNFLKQRLG"
     gene            2978..3358
                     /locus_tag="VF453_14410"
     CDS             2978..3358
                     /locus_tag="VF453_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEX7688903.1"
                     /translation="MPRLVLDESRIHPSVRGRVSDAGRAVIGEVEAAIAANAVVVVGM
                     GLNPFPRQARKWLDAHGVPYKYLGYGSYFSAWRTRLPLKLWTGWTTFPMVFVGGQFVG
                     GAQDLARLDPAALKTGTVRMAAGA"
     gene            3363..>4270
                     /locus_tag="VF453_14415"
     CDS             3363..>4270
                     /locus_tag="VF453_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7688904.1"
                     /translation="MRRLALASSLLLAAAVTGGAGAAAPAAGTTLVLHACRLPGLAQD
                     ARCGTLSRPLDPARPDGTRIDLQVAVLPAVARRKQPDPVFFFAGGPGQSAIGLAGIVE
                     ALLSHIDERRDIVLIDQRGTGHSAPLKCDTPSAEEAVARTLDRRALFAAMDACRAQLQ
                     RLPWGDLRFYTTSIAMADADAVRAALGAERVDLVGVSYGTRAALEYLRLYPRHVRRVV
                     LDSVVPPDMTLPESAAIDDQAAISKALQACADEPACARAHPVLDEAWRQLLASLPRSV
                     DVDDPVTGRPLHVTMTRDAVMGLAHAG"
BASE COUNT          581 a         1509 c         1470 g          710 t
ORIGIN      
        1 cgcccgtatg ctcgcgccct gcagcttcat gcccgcgatc agcacgcgct cttcaggcct
       61 gagttgcctg tacttgcttc cttcttccat cgcagcacct taccggaggt gaggtgttgc
      121 acttcagact tgaggtcgcc ctcccattcg tcaaattcct cgatgtccgg cttcggctcc
      181 ttcatcttct gctcttgctt ctcggtcgac agcgtctgcg cgatccgctc gaggctcggc
      241 gccctcgcga tgatttcctt gtccagcttt ctcttgcgga caacgatctt gccttgtttg
      301 tcgcttgagt tgttcatgac ttgacctccg ttactcggct ataggtggcc gtgacgtgct
      361 tgaagcgggc cccgttctca tcgctgtaat ccgctgcttc aagccggctc atgatgctag
      421 ccagttcgaa tccgctctcg gccaggaagc aaagcagcga aaaaagcttt ctctccggat
      481 ccgatgcaag cagcacgtac tggtcaaccg ctgccggcga gctcgataca tcccgttcaa
      541 agaccgcgta ggacgagtcg ccctcaccga agatctgcac ggacacgccc tggcggtagt
      601 agtgacgatc ctcgctcaat atgaaccgca tccactcggt caagcagttc agcgaaggag
      661 ctgcttcttc gatttgcttg tcaagagagt tcattgaata tctacacttc gcgctgcatg
      721 aatgaacgcc aggggttcgt tgtatcgggg tgtcgatcga tcggttggaa gaatagcccg
      781 gacccggacg agagcaatcg ccaggttggg ggtgagccgc gcttcacgct atttgcgcag
      841 gccctcggcg accagcgccg cctgcaccgc cggccgcgcc gtcacgcgcg cgcgccaggc
      901 cgcgaagtcg gcgaggcccg agaggtcgag gcccagcccg tcggcccagg tcgcgaccgt
      961 gtagaggtag gcgtcggcga cggtgaactg cgcacccatc aggtactcgc ggccggccag
     1021 cgccttgtcg acgtaggcga acttgtcctt cagtcgcgcg gcgaagattt ccttggcggc
     1081 ctgcggcatc tgcgcgttga acagcggcga gaagcccttg tgcagctcgg aggtgacgaa
     1141 gttgagccac tccatgagcc ggtagcgctc catcgtgccc gccgcgggcg cgaggccgct
     1201 ggccggcgcg tggtcggcga tgtactggac gatgaccggg ccctcggtca ggcgctggcc
     1261 gtcgtccagc tccagcatcg gcacctggcc cttgggcgcg acggcgtagt agtcgctgcc
     1321 gtcggcgacc agcgtgtggg tcttgagcgc ggtcttctcg aggtcgaact tgatgccggc
     1381 ctcgcgcagg acgatgtgcg gggacaggga gcaggcaccg gggctgtagt agagcttcat
     1441 ggcgggcgat cctttggcgt ggacgacggg aacggcgcga cgcggcgccg agcccgcaat
     1501 gatgcctctt cgcgccggcg ggcgtcggcc ggcgctcgcc gggtccggcg tgaggaattg
     1561 gcgccggcgt gcccgccccg caacgatcgc gcgttcgccg agcccagtcg tgcacgccgc
     1621 gtcccccggc gcggatgacg cggggccggc gggtcgacac catcgcgctc cgactccccg
     1681 gcctcgcctg tcatgcaccg ttcagcgggg cggttcagga tcgggccggg gagcgaacca
     1741 tcaacatcca tcgcaggagg acattcgcat gaaacgccat ctcgccgaac tgatcggcac
     1801 gttctggctg gtgctcgggg gctgcggcag cgcggtgctc gccgccgcct tcccgggcct
     1861 gggcatcggg ctgctggggg tctcgctggc cttcgggctg accgtcgtca ccatggccta
     1921 cgccatcggc cacgtcagcg gctgccacct caacccggcc atctcgatcg ggctgtgggc
     1981 gggccgtcgc ttcgagggcc gccaggtgct gccctacatc gtcgcgcagg tgctcggcgc
     2041 gacgctggcc ggcgcggcgc tgtacgcggt cgcgagcggc aaggccgggt tcgccgccgg
     2101 cggcttcgcg tccaacggct acgcgctgca ctcgccgggc ggctactcgc tcgcggcggg
     2161 gatggtgtgc gaggtcgtga tgtcggcggt gttcctgttc gtcatcatgg gcgcgacgga
     2221 tcgccgcgcg cccgccggct tcgcgccgct ggccatcggc ctgacgctga cgctgatcca
     2281 cctggtcagc attcccgtca ccaacacctc ggtcaacccg gcgcgcagca ccggcgtggc
     2341 cctgttcgcc gacgcgtggg cgctgcagca gctgtgggcg ttctgggtcg cgccgatcgc
     2401 gggcgcggtg atcggagcgc tcgcctaccg gttcgtcgcc gaggattgag tccgcacggg
     2461 atcgatcgcc ttcgttcaac cgcctgaaag gaaaacgcca tggacacgca acaactgatc
     2521 tccgaattcc tcgcctccga gcacggcgcg caggccgtgc aggcgctcgg cgcgcagggc
     2581 atcggccagg ccgacgctca gcagctgctc acgcacgcga ccggggccgc ggtcgaccac
     2641 gtcgaggagc agcacgccgg cctgatgggc gagcatcccg gcaagagctt cttcgccgcc
     2701 ttcgcggccg gcctcgtgcg cggggacggc ttcttcaagt cgctggtcga cggcggcgaa
     2761 ggggtgatca ccgggcgcat ggccgaggcc atcgctgcgc gcgcgggcat cgacccggcc
     2821 accgcctcga cgatcgccgc cgcggccacg ccctacgtgc tgaacttcct caagcagcgg
     2881 ctgggctagg aatccgggag ggcctccggc ggggatgcgc gcggcagctc cgctctgcta
     2941 cgctgcatcc cctcgcatcg ccgcagaaag gcccgccgtg ccccgcctcg tcctcgatga
     3001 atcgcgcatc catccgtctg tacgggggag ggtctccgac gccgggcggg cggtgatcgg
     3061 ggaggtcgag gccgccatcg cggccaacgc cgtcgtcgtg gtcggcatgg ggctgaaccc
     3121 gttcccgcgc caggcccgca agtggctcga cgcgcacggc gtgccctaca agtacctcgg
     3181 ctacggcagc tacttcagcg cgtggcgcac gcgcctgccg ctgaagctgt ggacgggctg
     3241 gacgacgttc ccgatggtct tcgtcggcgg ccagttcgtc ggcggcgcgc aggatctcgc
     3301 ccggctcgac cccgccgcgc tgaagacggg caccgtgcgg atggccgcgg gcgcctgagc
     3361 cgatgcgccg gctcgcgctc gcgtcgtcgc tgctcttggc cgcggccgtc acgggcggcg
     3421 ccggggccgc cgcgccggcg gccgggacga cgctcgtgct gcatgcctgc cgcctgcccg
     3481 gcctggcgca ggacgcccgc tgcggcacgc tgtcgcggcc gctggatccg gcgcgtcccg
     3541 acgggacccg catcgacctg caggtcgccg tgctgcccgc cgtcgcgcgc cgcaagcagc
     3601 ccgatcccgt cttcttcttc gccggcggcc cggggcagag cgccatcggg ctcgcgggca
     3661 tcgtcgaggc gctgctttcg cacatcgacg agcggcgcga catcgtgctg atcgaccagc
     3721 gcggcaccgg ccacagcgcg ccgctgaagt gcgacacgcc gtcggccgag gaagccgtcg
     3781 cccgcaccct ggaccgccgc gcgctgttcg ccgcgatgga cgcctgccgc gcgcagctgc
     3841 aacgcctgcc gtggggcgac ctgcgcttct acaccacttc catcgcgatg gccgacgccg
     3901 acgccgtgcg cgccgcgctg ggcgccgagc gcgtcgacct ggtgggcgtc agctacggca
     3961 cgcgagccgc cctggaatac ctccggctgt atccccggca tgtgcgccgc gtcgtgctgg
     4021 acagcgtcgt tccgcccgac atgacgctgc ccgagagtgc ggcgatcgac gaccaggcgg
     4081 cgatctcgaa ggcgctgcaa gcctgcgccg acgagcccgc ctgcgcgcgc gcgcatccgg
     4141 tgctggacga ggcctggcgg caactgctgg cttcgctacc gcgcagcgtc gatgtcgacg
     4201 atccggtcac gggccggccc ctgcacgtga cgatgacgcg cgacgcggtg atgggcctcg
     4261 cgcacgccgg
//
LOCUS       DASVLN010000600         1933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_517139, whole genome shotgun sequence.
ACCESSION   DASVLN010000600 DASVLN010000000
VERSION     DASVLN010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517139"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..1408
                     /locus_tag="VF453_14420"
     CDS             365..1408
                     /locus_tag="VF453_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX7688905.1"
                     /translation="MTDEIPVPRPIPELAAAGLDRERFHAEIVPRAEPVVLRGVVAHW
                     PAVAQGRASPEAIVAYLARLDTGAQYGAVLLPPEHEGRMHYRPGVDGFNFVRDQRSVS
                     AVLEQLARYSHFERPPSVALQCAPIPECLPGFTDDNPCPLLDAGVPPRLWLGNRFVTP
                     AHIDELDNLACVVAGRRRFTLFPPEQVANLYIGPLDFTPAGAPVSMASLHAPDFARHP
                     RLREALANARSAELGPGDALFIPAVWWHHVESFDLVNVLVNYWWEQPAAAMPGGRISP
                     TKSLLHALLSMRHLPPAHRKAWGEVFAHYVFTPESDPAAHLPQARRGVLGPLPDEAVD
                     RMLAATGARQARS"
     gene            complement(1433..>1933)
                     /locus_tag="VF453_14425"
     CDS             complement(1433..>1933)
                     /locus_tag="VF453_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688906.1"
                     /translation="AEAALHATRGADDTLLRASIALRAVPGEFDLAWVGRLSQVAAAL
                     PAQRPVLEPPEIADRLGALGKLLACAEFNVDGQIATCDHPAMRKALELARYLLGRLRA
                     GWNESGLWSPRATSQGDAAEVEALEDELYFRLRGIQRATLLAAGKLPIEEQARLEDLC
                     ESLATG"
BASE COUNT          241 a          743 c          673 g          276 t
ORIGIN      
        1 gccgtggatg ctgctcgggt tgccgaaggt gtcggccatg gcggcaagcg cggcggcgcg
       61 cgcctggggc agcacggggg tggtggcgtt ggcgtcgaga tagagttcca tggatcagga
      121 ttcttctcgc gtccgcgcgc aaagggcgtg cgttggaggt cccgtgccgc tgtcgtgatg
      181 gaatcaaatt ccattgaggt ggctgaatcg ggaaaatttt tccgcgggac cgcgaggtcg
      241 cgccgccttc gtgaaatccg gcgcaggccg cggacgaggc acgcaaccgt gcgaattcac
      301 gccgccgcgc gggcgggcgc ccgcaggccg gctcctatac tcgtccgacg cctcccccca
      361 gaccatgacc gacgagatcc ccgtcccccg gcccatcccc gaactcgctg ccgccggcct
      421 cgatcgcgaa cgcttccacg ccgagatcgt gccccgcgcc gagcccgtgg tgctgcgcgg
      481 cgtggtcgcg cactggccgg cggtggcgca gggccgggcg tcgccggagg cgatcgtcgc
      541 ctacctcgcg cggctcgaca ccggcgcgca gtacggcgcc gtgctgctgc cgcccgagca
      601 cgaaggccgc atgcactatc gcccgggcgt cgacgggttc aacttcgtgc gcgaccagcg
      661 ctcggtctcc gcggtgctgg agcagctcgc ccgctactcg cacttcgagc gcccgccctc
      721 ggtcgcgctg cagtgcgcgc cgatccccga gtgcctgccc ggcttcaccg acgacaaccc
      781 gtgcccgctg ctggacgccg gcgtgccgcc gcggctatgg ctcggcaacc gcttcgtgac
      841 gcccgcccac atcgacgagc tcgacaacct cgcctgcgtc gtcgccggcc ggcgccgctt
      901 cacgctcttc ccgccggagc aggtcgccaa tctctacatc ggcccgctgg acttcacgcc
      961 cgccggcgcg ccggtcagca tggcctcgct gcacgcgccc gatttcgcgc gccacccgcg
     1021 cctgcgcgag gcgctcgcca atgcccggtc ggccgaactc ggcccgggcg acgcgctgtt
     1081 catcccggcg gtgtggtggc accacgtcga gtccttcgac ctcgtcaacg tgctcgtgaa
     1141 ctactggtgg gagcagccgg ccgccgcgat gcccggcggg cgcatctcgc ccaccaagtc
     1201 gctgctgcac gcgctcctga gcatgcgcca cctgccgccc gcgcaccgca aggcctgggg
     1261 cgaggtgttc gcgcactacg tcttcacgcc ggagtccgat cccgccgcgc acctgccgca
     1321 ggcgcgccgc ggcgtgctcg ggccgctgcc cgacgaggcg gtcgaccgca tgctggcggc
     1381 gaccggggcg cggcaggcga gatcctgaag ttgctgcgga gccggcggcg cttcagccgg
     1441 tcgcgagcga ctcgcagaga tcctcgagcc gcgcctgctc ctcgatcggc agcttgcccg
     1501 cggccagcag cgtggcgcgc tggatgccgc gcaggcggaa gtagagctcg tcctcgagcg
     1561 cctcgacctc ggccgcgtcg ccctgggatg tcgcgcgcgg gctccacagg ccgctctcgt
     1621 tccagcccgc gcgcaggcgc ccgagcaggt agcgtgcgag ctccagcgcc ttgcgcatcg
     1681 ccggatggtc gcaggtcgcg atctggccgt cgacgttgaa ctcggcgcac gcgagcagct
     1741 tgcccagcgc gcccagccga tcggcgatct ctggcggttc cagcaccggc cgctgcgccg
     1801 gcagcgccgc ggcgacctga gacagccggc ccacccacgc gagatcgaac tcgcccggga
     1861 cggcgcgcag cgcgatgctc gcgcgcagca gcgtgtcgtc cgcgccgcgc gtggcatgca
     1921 gcgcggcctc cgc
//
LOCUS       DASVLN010000601         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_246308, whole genome shotgun sequence.
ACCESSION   DASVLN010000601 DASVLN010000000
VERSION     DASVLN010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246308"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1659)
                     /locus_tag="VF453_14430"
     CDS             complement(<1..>1659)
                     /locus_tag="VF453_14430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7688907.1"
                     /translation="GAGSAGDASQPRQGSLMAVGRDLVEPESMAEYDRLQAALRNRTR
                     IEVRYAVRHHESGRRWLMTRVEPAELGPQRGAFSVVTVDVTEEETARRRSHDLLTELS
                     TILDVSPAGIAYLRGDRLVRCNRRFESLLNGAAGAPGSPGGRSLESLLRDAGVAPEAV
                     EQVQRAVRETGALETELLLPAGPQRPAPAWCSLALRRTHETTPGAVGLVVVLADATRL
                     KSQQAELEALARERELMFNLSDVGIVYQRDGHVERANQAMADLLGVAPEALKGLPMAA
                     LYESEAAWRHYQQLEAHDMATLGRSHGERRVRRQDGELFWAQVSQRPVAFDRPAAGTI
                     LSFVNIDDRQRARESLVQQADRTRAILDSVLVGIVTVGDAGIEWMNRSARRMFGGELA
                     DFVGVPIAVVATDELDHPLRRTDYGETLEEGQAETFECRLRARDGRNFWVVGNVVVTG
                     QDTAHRQLTYALLDIERRRQAEVSIVQARASLQRIIETAPLAIALFDAASQQVVQLNQ
                     MAATFAGRPMQEIVGRVPSQWLPGPEGAKLAADLHRALASPDAV"
BASE COUNT          198 a          602 c          638 g          221 t
ORIGIN      
        1 tgcacggcgt cgggcgaggc gagcgcgcgg tgcaggtcgg ccgccagctt ggcgccctcc
       61 ggcccgggca gccactgcga gggcacgcgg ccgacgatct cctgcatcgg ccggccggcg
      121 aaggtggcgg ccatctggtt cagctgcacg acctgctggc tcgcggcgtc gaagagggcg
      181 atcgcgagcg gcgccgtctc gatgatgcgc tgcaggctcg cacgcgcctg gacgatgctg
      241 acctcggcct ggcggcggcg ctcgatgtcc agcagcgcgt acgtgagctg gcggtgggcg
      301 gtgtcctggc ccgtgacgac gacgttgccg acgacccaga agttgcgccc gtcgcgcgcg
      361 cgcagccggc actcgaaggt ctcggcctgg ccttcctcca gcgtctcgcc gtagtcggtg
      421 cggcgcagcg ggtggtcgag ctcgtcggtc gcgacgaccg cgatcggcac gccgacgaag
      481 tccgccagct cgccgccgaa catgcggcgc gccgagcggt tcatccactc gatgccggcg
      541 tcgccgaccg tcacgatgcc cacgagcacc gagtccagga tcgcgcgcgt gcggtcggcc
      601 tgctgcacga gcgactcgcg cgcgcgctgg cggtcgtcga tgttgacgaa ggacaggatg
      661 gtgccggcgg ccgggcggtc gaaggcgacg gggcgctggc tcacctgagc ccagaacagc
      721 tcgccgtcct ggcggcgcac gcggcgctcc ccgtgcgagc gcccgagcgt ggccatgtcg
      781 tgcgcctcga gctgctggta gtggcgccag gcggcctcgc tctcgtagag cgcggccatc
      841 ggcaggccct tgagcgcctc gggcgcgacg cccagcaggt cggccatcgc ctggttggcg
      901 cgttcgacgt ggccgtcgcg ctggtagacg atgccgacgt cggagaggtt gaacatcagc
      961 tcgcgctcgc gcgccagcgc ctcgagctcg gcctgctggg acttcagccg cgtcgcgtcg
     1021 gcgagcacga ccaccaggcc gaccgcgccc ggcgtcgtct cgtgcgtgcg ccgcagcgcg
     1081 agcgagcacc aggccggcgc cggccgctgc gggccggcgg gcagcagcag ctcggtctcg
     1141 agcgcgccgg tctcgcgcac cgcgcgctgc acctgctcca cggcctcggg agcgacgccg
     1201 gcgtcgcgca gcaggctctc caacgagcgc ccgccgggcg agcccggcgc gcccgcggcg
     1261 ccgttgagca gcgattcgaa gcggcggttg cagcgcacga gccggtcgcc gcgcaggtag
     1321 gcgatgcccg cggggctgac gtcgaggatg gtggacagct ccgtgagcag gtcgtggctg
     1381 cggcggcggg cggtttcctc ctcggtcacg tcgacggtga cgaccgagaa cgcgccgcgc
     1441 tgcgggccga gttccgcggg ctcgacgcgc gtcatgagcc agcgccggcc gctctcgtgg
     1501 tggcgcacgg cgtagcgcac ctcgatgcgc gtgcggttgc gcagcgcggc ctgcaggcgg
     1561 tcgtattcgg ccatcgactc gggctcgacc aggtcgcggc cgacggccat caggctgccc
     1621 tggcgcggct gcgacgcgtc gcccgccgat cccgcgcca
//
LOCUS       DASVLN010000602         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_462803, whole genome shotgun sequence.
ACCESSION   DASVLN010000602 DASVLN010000000
VERSION     DASVLN010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462803"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..2009)
                     /locus_tag="VF453_14435"
     CDS             complement(138..2009)
                     /locus_tag="VF453_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX7688908.1"
                     /translation="MDSAHRRQALLPLTLGALGVVYGDIGTSPLYTMGTIFSTVGGVP
                     MTRGNLVGAASTIFWALMLVVTLKYVLLILRADNRGEGGIMALTALAVQAARRHHGTL
                     LLLLGLFGAALFYGDSVITPAMSVLSAIEGLEVFEPGLKKFVLPVAVGVLFALFLMQR
                     KGTGTVGKLFGPIIAIWFAVLAVTGAQHIAAEPAILAAFNPLHAIEFLVDRGIFVFAA
                     VGAIVLALTGAEALYADMGHFGRRPIQIAWLGLVMPSLALQYLGQAALLMRDPKAADS
                     PFFHMFPQHLLIPVVVLATAATVIASQAVISGAFSMTRQAIQLGFLPRMQVVFTSAKE
                     AGQIYLPRVNWVLLCAVIAAAVGFGSSDRMASAYGIAVTVTMLITTMLTFFVVRHAWG
                     LPAWLAWAGTGVFFAFDALLVTSCSIKFVEGGWFPIAMGLAIFTVMSTWKRGRALLLE
                     SIRQDGLELLPFIEALAADAVQRVPRTAVFTVADPTTVPQAMLHNLKHNCVLHEHNVI
                     LSVIFHEEPYIEDDATAEVVPLAPGFWRVSLNYGFMNVPDVPGALARIAGHGIDFEPM
                     RTSYFISREVVVPTRGTGMAQWRERLFATMSRNAGSAVEFFKLPNNAVIELGTRVQI"
     gene            2258..2596
                     /locus_tag="VF453_14440"
     CDS             2258..2596
                     /locus_tag="VF453_14440"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEX7688909.1"
                     /translation="MILNTPHARLYLADDEVARLSDARESRIEVTQGFVWVTIDGDRG
                     DIVLGAGESFVVGASDVATLSAIRGAAAVNVHARSGARARPAAARHPHQPSRLRQLMA
                     GISISPVSYA"
     gene            complement(2597..>3100)
                     /locus_tag="VF453_14445"
     CDS             complement(2597..>3100)
                     /locus_tag="VF453_14445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7688910.1"
                     /translation="RAGDAEPQRAAVEAGALVEEVVGAQSAAWPGVAIVVQPLPQVEA
                     DAGLLRQVFVNLVGNACKFAAGGVLPRVEIGHGQTPCGPAFYVRDNGVGFAPEDAGRL
                     FRPFERLHGARFEGFGVGLSIVRRIVEHHGGRVWAESSPGQGATFRFTLGGDGATAER
                     PGAALQA"
BASE COUNT          522 a         1079 c         1064 g          435 t
ORIGIN      
        1 gcggaggtac gtcaatcggg catgcttatg ggtgttcatc cggttccggc tttgaagttg
       61 acggggggtt tggcgattcc cagtctctca aaacctctcc gggtgaacac ccgcaacaac
      121 ctattgaacc ttcacaccta gatctgcacg cgggtaccga gttcgatgac ggcgttgttc
      181 ggcagcttga agaattcgac cgcgctgccc gcgttgcgcg acatcgtcgc gaacaggcgt
      241 tcgcgccact gcgccatgcc ggtgccgcgc gtgggcacga cgacctcgcg gctgatgaag
      301 tagctggtcc gcatcggctc gaagtcgatg ccgtggcccg cgatgcgcgc gagcgcgccc
      361 ggcacgtcgg ggacgttcat gaagccgtag ttcaggctca cgcgccagaa gccgggcgcg
      421 agcgggacga cctccgccgt cgcgtcgtcc tcgatgtagg gctcctcgtg gaagatcacc
      481 gacaggatca cgttgtgctc gtgcagcacg cagttgtgct tgaggttgtg cagcatcgcc
      541 tgcggcacgg tggtgggatc ggccaccgtg aagaccgccg tgcgcggcac ccgctgcacg
      601 gcgtcggcgg ccagcgcctc gatgaagggc agcagctcga ggccgtcctg gcggatcgat
      661 tccagcagca gcgcgcggcc gcgcttccac gtcgacatca ccgtgaagat cgccagcccc
      721 atcgcgatcg ggaaccagcc gccttcgacg aacttgatcg agcacgaggt cacgagcagc
      781 gcgtcgaacg cgaagaacac gccggtgccc gcccacgcga gccaggcggg cagtccccag
      841 gcgtggcgca cgacgaagaa ggtgagcatg gtcgtgatca gcatcgtcac cgtcacggcg
      901 atgccgtagg ccgaggccat gcggtccgag ctgccgaagc ccaccgccgc ggcgatcacc
      961 gcgcacagca gcacccagtt gacgcgcggc aggtagatct ggcccgcctc cttcgcggag
     1021 gtgaagacga cctgcatgcg cggcaggaag ccgagctgga tcgcctggcg cgtcatcgag
     1081 aacgcgccgg agatcaccgc ctgggacgcg atcacggttg ccgccgtcgc cagcaccacg
     1141 accgggatca acaggtgctg cgggaacatg tggaagaacg ggctgtcggc ggccttcggg
     1201 tcgcgcatca gcagcgccgc ctggccgagg tactgcagcg cgagggaggg catgacgagg
     1261 ccgagccagg cgatctggat cgggcggcgg ccgaaatggc ccatgtcggc gtacagcgcc
     1321 tcggccccgg tcagggcgag cacgatcgcg ccgacggccg cgaacacgaa gatgccgcgg
     1381 tcgacgagga actcgatcgc atgcagcgga ttgaacgcgg cgaggatcgc cggctccgcg
     1441 gcgatgtgct gcgcgcccgt gaccgccagc accgcgaacc agatggcgat gatgggcccg
     1501 aacagcttgc ccaccgtgcc ggtgcccttg cgttgcatca ggaacagcgc gaacagcacg
     1561 cccaccgcga ccggcagcac gaatttcttc aacccgggct cgaagacctc caggccttcg
     1621 atcgcgctga gcacggacat cgccggcgtg atgacgctgt cgccgtagaa cagcgccgcg
     1681 ccgaacaggc cgagcagcag cagcagcgtg ccgtggtggc gtcgcgcggc ctggaccgcg
     1741 agcgccgtca gcgccatgat gccgccctcg ccgcggttgt ccgcgcgcag gatcagcagc
     1801 acgtacttca gcgtcaccac cagcatcagc gcccagaaga tcgtcgacgc ggcgccgacg
     1861 aggttcccgc gcgtcatggg cacgccgccg accgtgctga agatcgtgcc catcgtgtac
     1921 agcgggctgg tgccgatgtc gccgtagacg acgccgagcg ctccgagggt gaggggcagg
     1981 agcgcctgcc gacgatgtgc cgagtccatc cgggattgtt gtcgtggaag tggggcggct
     2041 ttttacgccg tcggtataaa ttttccataa agacggggcg ggccatgggg cgttcgccat
     2101 gcgtaaacgg aatgggtgca atgagaaagc atcgttggag tttgtcgggg aaaaccccga
     2161 tgattcactc acccgatgca aaggccgatg tgcgtggcga aagcgccacg gatgcgatcc
     2221 cggcatgggg agatgtcacc agaaccggag tacgaccatg atcctcaaca cccctcacgc
     2281 acgcctgtac ctcgccgacg acgaagtcgc ccgcctgagc gatgcccgcg aatcccgcat
     2341 cgaggtcacg cagggcttcg tctgggtcac gatcgacggc gaccgtggcg acatcgtgct
     2401 cggcgccggc gagtccttcg tcgtgggtgc ctccgacgtc gcgacgctga gcgccatccg
     2461 cggcgccgcg gcggtcaatg tccacgcccg ctcgggcgcc cgcgcccgtc cggcggcggc
     2521 gcgccacccg caccagccga gccgcctgcg ccagctgatg gccggcatct cgatcagccc
     2581 cgtctcctac gcctgatcag gcctgcagcg cggcgcccgg ccgctcggcg gtcgccccgt
     2641 cgccgccgag cgtgaaccgg aaggtcgcgc cctggccggg gctgctttcg gcccagacgc
     2701 ggccgccgtg gtgctcgacg atgcgccgca cgatcgacag tcccacgccg aagccctcga
     2761 agcgggcgcc gtgcaggcgc tcgaacgggc ggaacagccg cccggcgtcc tcgggcgcga
     2821 agcccacgcc gttgtcgcgc acgtagaagg ccgggccgca gggcgtctgc ccgtggccga
     2881 tctccacccg cggcagcacc ccgccggcgg cgaacttgca cgcgttgccg accaggttga
     2941 cgaagacctg gcgcagcagc ccggcgtcgg cctccacctg cggcagcggc tggacgacga
     3001 tcgcgacgcc cggccacgcc gccgactgcg ctcccaccac ctcctccacg agcgcgcccg
     3061 cctcgaccgc ggcgcgctgc ggctcggcgt cgccggcgcg
//
LOCUS       DASVLN010000603         7024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_31797, whole genome shotgun sequence.
ACCESSION   DASVLN010000603 DASVLN010000000
VERSION     DASVLN010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31797"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..548
                     /locus_tag="VF453_14450"
     CDS             159..548
                     /locus_tag="VF453_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688911.1"
                     /translation="MLALSPGQFEEMEANFRRTRRDRLAAEFVRGYPGLTAGVSQDDV
                     IAYLDVVIADSGPPDRKLDEEIIEFAAIGLLCLGLAAEQPERVALVRRVVADVYTMAR
                     RRLDFIWTRLLPEYAADRGRSAQRRRG"
     gene            complement(631..1941)
                     /gene="gltA"
                     /locus_tag="VF453_14455"
     CDS             complement(631..1941)
                     /gene="gltA"
                     /locus_tag="VF453_14455"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX7688912.1"
                     /translation="MTPSDVKATLSFSDGSPSMELPIYKGTIGPDVIDIRKLYGQTGK
                     FTYDPGFLSTASCNSAITYIDGDKGELLYRGYPIDQLAVNCDFLETCHLLLYGELPNA
                     EQKASFTNRVTNHTMVHEQMQFFLRGFRRDAHPMAVMTGLVGALSAFYHDSTDINNAE
                     HREIAAIRLIAKMPTLVSMAYKYSIGQPFIYPKNDLSYAGNFMRMMFATPCEEYKVSP
                     VIERALDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAA
                     LNMLEDIQRQGGVEKIGEFISKVKDKSSNVKLMGFGHRVYKNYDPRAKLMRETCHEVL
                     NELGLHNDPLFKLAMSLEKIALEDDYFVARKLYPNVDFYSGIVQRAIGIPVSLFTAIF
                     ALARTVGWIAQLNEMIADPEYKIGRPRQLYSGAAPRTVKPLAER"
     gene            complement(1938..2234)
                     /locus_tag="VF453_14460"
     CDS             complement(1938..2234)
                     /locus_tag="VF453_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX7688913.1"
                     /translation="MDTIVDPTELNRLRWRCKRGLLENDLFIERFFSEHGESLTARQV
                     AGLTALMELPDNDLLDLFLARREPDGALDSPEVKEVLALARRPAAVLLKKGNPQ"
     gene            complement(2290..2994)
                     /locus_tag="VF453_14465"
     CDS             complement(2290..2994)
                     /locus_tag="VF453_14465"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX7688914.1"
                     /translation="MTKRTFEIYRYDPDKDDKPYMQTIELELDGSERMLLDALIKLKA
                     VDPTISYRRSCREGVCGSDAMNINGKNGLACLTNLRSLPEVIKLKPLPGLPVVRDLIV
                     DMTQFFKQYDSIKPYLVNDTPPPEKERLQSPEEREELNGLYECILCASCSTSCPSFWW
                     NPDKFVGPAGLLQAYRFIADSRDQATGERLDNLEDPYRLFRCHTIMNCVDVCPKGLNP
                     TKAIGKIKEMMVRRAV"
     gene            complement(3014..4819)
                     /gene="sdhA"
                     /locus_tag="VF453_14470"
     CDS             complement(3014..4819)
                     /gene="sdhA"
                     /locus_tag="VF453_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX7688915.1"
                     /translation="MTAASSITKRKFDVVIVGAGGSGMRASLQLSRAGLNVAVLSKVF
                     PTRSHTVAAQGGIGASLGNMSEDNWHFHFYDTVKGSDWLGDQDAIEFMCREAPKVVYE
                     LEHFGMPFDRNPDGTIYQRPFGGHTANYGEKAVQRACAAADRTGHALLHTLYQQNVKS
                     RTQFFVEWMALDLVRDAEGDVVGVTALEMETGDLHILEAKTVLLATGGAGRIYAASTN
                     AFINTGDGLGMAARAGIPLEDMEFWQFHPTGVAGAGVLLTEGCRGEGAILRNADGERF
                     MERYAPHYKDLAPRDFVSRCMDQEIKEGRGCGPNKDYIHLDMTHLGTETIMKRLPSVY
                     EIGHNFANVDITKEPIPVVPTIHYQMGGIPTNIHGQVVAPKDGDPNSVINGLYAVGEC
                     SCVSVHGANRLGTNSLLDLVVFGRAAGNHIVASDLRNRAHKPLPADAADRTLARLARL
                     ESSTSGEYAQTVANDIRSTMQKHAGVFRTQELLDAGVKAIAALRERVGGIALKDKSKV
                     FNTARIEALEVENLIEAAQATIVSAAARTECRGAHVVVDYERPDTDAEFPNGRNDRDW
                     LRHTLWFSEGSRLDYKPVKLKPLSTESIPPAVRSF"
     gene            complement(4824..5186)
                     /gene="sdhD"
                     /locus_tag="VF453_14475"
     CDS             complement(4824..5186)
                     /gene="sdhD"
                     /locus_tag="VF453_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX7688916.1"
                     /translation="MATNYGTKRVVVGAHYGLRDWLVQRATAVVMAIFTVALVLQLLM
                     PGPLDYDRWAGIFAHQWMKLLTFVTIVSILWHAWVGVRDVLMDYVQAVAWRLVLQVAA
                     IVWLVGCAGWALQVIWRL"
     gene            complement(5200..5628)
                     /gene="sdhC"
                     /locus_tag="VF453_14480"
     CDS             complement(5200..5628)
                     /gene="sdhC"
                     /locus_tag="VF453_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX7688917.1"
                     /translation="MADAKAIKKRPGGMTFADATSYRLPPAGVISILHRISGLLLIVL
                     MPFVIWLFDNSVSDQVSFDVFRSAFLAGIGFVPAWLVKIATLVLIWAFLQHFCAGVRH
                     LYMDFTHNVTKEFGRQSALVALGAAVVLTIAFGLKLFGVY"
     gene            complement(5820..6554)
                     /locus_tag="VF453_14485"
     CDS             complement(5820..6554)
                     /locus_tag="VF453_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7688918.1"
                     /translation="MSSDPSGPAFSPLYQQIKGLIMRSLQDGEWKPGEAIPAETDLAQ
                     RYRVSQGTVRKAIDELATENLVVRRQGKGTFVATHAEEKIQYRFLRLAPDDQRPRGMT
                     RHVLDCRRMRAPAPIARALDLKGAEGALLVRRTLAQDGEPVVLDDIWLPAPLFKGLTA
                     ERITDYVGPMYRLFETEFDVRMIRAEEKIRAVAADEATAGVLRVPVGTPLLSVERLSF
                     TYGDKPVELRRGLYRTDRHYYRNSLS"
     gene            6908..>7024
                     /locus_tag="VF453_14490"
     CDS             6908..>7024
                     /locus_tag="VF453_14490"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913700.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7688919.1"
                     /translation="MSKTPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVIL"
BASE COUNT         1032 a         2257 c         2512 g         1223 t
ORIGIN      
        1 cgcccgtatg ctcgcgccct gcagcttcat gcccgcgatc agcacgcgct cttcaggcct
       61 gagttgcctg tacttgcttc cttcttccat cgcagcacct taccggaggt gaggtgttgc
      121 acttcagact tgaggtcgcc accggatagg atgacccgat gctggcactc tctccaggtc
      181 agttcgagga gatggaggcg aacttccgtc ggacccggcg cgatcggctc gccgccgagt
      241 tcgttcgcgg ctacccgggc ttgacggccg gcgtttcgca agacgatgtc atcgcctatc
      301 tcgacgtcgt catcgccgat agcggcccgc cggaccgcaa gcttgatgag gaaatcatcg
      361 agttcgccgc catcggtctg ctgtgcctgg gtcttgcggc ggaacagccg gagcgcgtcg
      421 cgcttgtgcg ccgcgtggtg gcggatgtct acacgatggc ccgtagacgg ctggacttca
      481 tctggacgcg cctgctgccc gagtacgcgg ccgacagagg ccggtccgct cagcggcgac
      541 gcggctgaag gtccgcggaa tggaaagggc cgcgcgaggc ggccctttgg gcgatgccga
      601 cccgcgtgga cgcgggctcg gcggcgcggc tcagcgctcc gccagcggct tgaccgtgcg
      661 cggcgcggcg ccggaataca gctggcgcgg gcggccgatc ttgtactcgg ggtcggcgat
      721 catctcgttc aactgggcga tccagcccac cgtgcgcgcg agcgcgaaga tggccgtgaa
      781 caggctgacc gggatgccga tcgcgcgctg cacgatgccc gagtagaagt cgacgttcgg
      841 gtagagcttg cgggcgacga agtagtcgtc ttccagcgcg atcttctcga gcgacatcgc
      901 gagcttgaac agcgggtcgt tgtgcaggcc cagctcgttg aggacctcgt ggcaggtctc
      961 gcgcatcagc ttggcgcgcg ggtcgtagtt cttgtagacg cggtggccga agcccatgag
     1021 cttgacgttc gagctcttgt ccttgacctt ggagatgaac tcgccgatct tctcgacgcc
     1081 gccctggcgc tggatgtcct ccagcatgtt cagcgcggcc tcgttcgcgc ccccgtgggc
     1141 cgggccccac aggcaggcga cgccggcggc gatcgcggcg aacgggttgg tgcccgacga
     1201 gccgcacagg cgcacggtgg acgtcgacgc gttctgctcg tggtcggcgt gcaggatgaa
     1261 gatgcggtcc agcgcgcgct cgatgaccgg gctgaccttg tactcctcgc acggcgtggc
     1321 gaacatcatg cgcatgaagt tgccggcgta gctcaggtcg ttcttcgggt agatgaacgg
     1381 ctggccgatc gagtacttgt aggccatcga cacgagcgtc ggcatcttcg cgatgaggcg
     1441 gatcgcggcg atctcgcggt gctcggcgtt gttgatgtcg gtggagtcgt gatagaaggc
     1501 cgacagcgcc ccgaccaggc ccgtcatcac ggccatcgga tgcgcgtcgc ggcggaagcc
     1561 gcgcaggaag aactgcatct gctcgtgcac catcgtgtgg ttggtcacgc gattggtgaa
     1621 cgacgccttc tgctcggcgt tcggcagctc tccgtacagc agcaggtggc aggtctcgag
     1681 gaagtcgcag ttcaccgcca gctggtcgat cggatagccg cggtacagca gctcgccctt
     1741 gtcgccgtcg atgtaggtga tggccgaatt gcacgacgcc gtggacagga agcccgggtc
     1801 gtaggtgaac ttgccggtct ggccgtacag cttgcggatg tcgatcacgt cgggcccgat
     1861 cgtgcccttg tagatcggca gctccatgct ggggctgccg tcggagaacg acagggtcgc
     1921 tttgacgtcg gagggggtca ttggggattg cctttcttga ggaggacggc agcaggccgg
     1981 cgtgcgagcg ccagcacttc tttcacttcg ggactgtcga gcgcgccatc cggttcgcgg
     2041 cgcgcgagga acaggtcgag caggtcgttg tcgggcagct ccatcagggc cgtcaggccc
     2101 gcgacctgcc gcgcggtcag cgactcgccg tgctcggaga agaaccgttc gatgaacagg
     2161 tcgttctcca gcaggccgcg cttgcaccgc cagcgcaggc ggttcagctc ggtcgggtcg
     2221 acgatggtgt ccattgtggt cccggtcggc gaggtttgct ggggagggct ctctggtcgc
     2281 gccggggcgt cagacggcgc gtcgcaccat catttccttg atcttgccga tcgccttcgt
     2341 cgggttcagg cccttcgggc agacgtcgac gcagttcatg atggtgtggc agcggaagag
     2401 gcggtacgga tcctcgaggt tgtcgaggcg ctcgccggtg gcctggtcgc gactgtccgc
     2461 gatgaagcga taggcctgca gcaggccggc ggggccgacg aacttgtcgg ggttccacca
     2521 gaagctcggg cacgaggtgg agcagctcgc gcacaggatg cactcgtaca ggccgttgag
     2581 ctcctcgcgc tcctcgggcg actgcaggcg ctccttctcg ggcggcggcg tgtcgttgac
     2641 gaggtagggc ttgatcgagt cgtactgctt gaagaactgc gtcatgtcga cgatcaggtc
     2701 gcgcacgacg ggcaggcccg gcagcggctt gagcttgatc acctccggca gcgagcgcag
     2761 gttggtcagg caggccaggc cgttcttgcc gttgatgttc atcgcgtccg agccgcacac
     2821 gccttcgcgg cacgagcggc ggtacgagat cgtcgggtcg accgccttca gcttgatcag
     2881 cgcgtccagc agcatgcgct ccgagccgtc gagctccagc tcgatggtct gcatgtaggg
     2941 cttgtcgtcc ttgtcggggt cgtagcggta gatctcgaag gtgcgtttgg tcatggctgg
     3001 atctctttcg cgttcagaac gaacggacgg cgggcgggat cgactcggtg gacagcggct
     3061 tcaacttcac cggcttgtag tcgaggcggc tgccctcgct gaaccacagc gtgtggcgca
     3121 gccagtcgcg gtcgttgcgg ccgttgggga actcggcgtc ggtgtccggg cgctcgtagt
     3181 cgacgaccac gtgggcgccg cggcactcgg tgcgcgcggc ggcggagacg atcgtcgcct
     3241 gcgcggcctc gatcaggttc tcgacctcca gcgcctcgat gcgcgccgtg ttgaagacct
     3301 tggacttgtc cttcagcgcg atgccgccga cacgctcgcg cagcgcggcg atcgccttca
     3361 cgccggcgtc cagcagctcc tgcgtgcgga acacgccggc gtgcttctgc atcgtggagc
     3421 ggatgtcgtt ggccaccgtc tgcgcgtact cgcccgaggt gctcgattcg agccgcgcca
     3481 ggcgcgcgag cgtgcggtcg gcggcgtcgg ccggcagcgg cttgtgcgcg cggttgcgca
     3541 ggtcggacgc gacgatgtgg ttgcccgcgg cgcggccgaa gacgaccagg tccagcagcg
     3601 aattggtgcc caggcggttc gcgccgtgca cgctcacgca ggagcactcg cccaccgcgt
     3661 acaggccgtt gatcacgctg ttcgggtcgc cgtccttcgg tgccacgacc tggccgtgga
     3721 tgttggtcgg gatgccgccc atctggtagt ggatcgtcgg caccacgggg atcggctcct
     3781 tcgtgatgtc gacgttggcg aagttgtggc cgatctcgta gacgctgggc aggcgcttca
     3841 tgatcgtctc ggtgcccagg tgcgtcatgt cgaggtggat gtagtccttg ttcggaccgc
     3901 agccgcgccc ttccttgatc tcctggtcca tgcagcgcga gacgaagtcg cgcggcgcca
     3961 gatccttgta gtgcggggcg tagcgctcca tgaagcgctc gccgtcggcg ttgcgcagga
     4021 tcgccccctc gccgcggcag ccttccgtca gcagcacgcc ggcgccggcc acgccggtgg
     4081 ggtggaactg ccagaactcc atgtcctcga gcgggatgcc cgcgcgcgcg gccatgccca
     4141 ggccgtcgcc cgtgttgatg aaggcgttgg tcgacgcggc gtagatgcgg cccgcgccgc
     4201 ccgtggccag cagcaccgtc ttggcctcga ggatgtgcag gtcgcccgtc tccatctcga
     4261 gcgccgtcac gcccaccacg tcgccctcgg cgtcgcgcac gaggtcgagc gccatccact
     4321 cgacgaagaa ctgcgtgcgc gacttgacgt tctgctggta cagcgtgtgc agcagcgcgt
     4381 ggccggtgcg gtcggcggcg gcgcaggcgc gctggacggc cttctcgccg tagttggcgg
     4441 tgtggccgcc gaacgggcgc tggtagatcg tgccgtccgg gttgcggtcg aacggcatgc
     4501 cgaagtgctc gagctcgtag acgaccttgg gcgcctcgcg gcacatgaac tcgatcgcgt
     4561 cctggtcgcc gagccagtcg gagcccttga cggtgtcgta gaagtggaag tgccagttgt
     4621 cctcgctcat gttgccgagc gaggcgccga tgcccccctg cgcggcgacg gtgtgcgagc
     4681 gcgtcgggaa caccttggac agcacggcga cgttgaggcc ggcgcgcgac agctgcagcg
     4741 acgcgcgcat gcccgagccg cccgcgccga cgatgacgac gtcgaacttg cgcttggtga
     4801 tggaagaagc ggcggtcatg ttctcagagt ctccagatca cttgcagggc ccagccggcg
     4861 cagccgacga gccagacgat ggcggccacc tgcagcacga gccgccaggc cacggcttgg
     4921 acgtagtcca tcagcacgtc gcgcacgccc acccaggcgt gccacaggat cgagacgatg
     4981 gtcacgaagg tgagcagctt catccactgg tgggcgaaga tgccggccca gcggtcgtag
     5041 tccagcgggc cgggcatcag cagctgcagc acgagggcga ccgtgaagat cgccatgacg
     5101 accgcggtcg cccgctgcac cagccagtcg cgcaggccgt agtgcgcgcc gacgacgacg
     5161 cgcttggttc cgtagttggt tgccatgggg ctgcttccct cagtagacgc cgaacagctt
     5221 gaggccgaac gcgatggtga gcacgacggc cgcaccgagc gcgaccagcg ccgactggcg
     5281 gccgaactcc ttggtgacgt tgtgcgtgaa atccatgtag aggtggcgca cgcccgcgca
     5341 gaagtgctgc aggaaggccc agatcagcac gagcgtggcg atcttcacca gccacgccgg
     5401 cacgaagccg atgccggcga ggaacgcgct gcggaagacg tcgaagctca cctggtcgct
     5461 gacgctgttg tcgaacagcc agatcacgaa cggcatcagc acgatcagca gcaggccgct
     5521 gatgcggtgc aggatcgaga tgacgcccgc gggcggcagc cggtatgagg tcgcgtcggc
     5581 gaatgtcatt ccgccgggtc gtttcttgat cgccttcgcg tcagccatgg ttggtcatcc
     5641 ttgtcgttat gcagatccgg cggcgcggga gtccgccggc gttgggcgcg gcgccgccgg
     5701 caggggccgg gcaccggata aacccgcgaa ttttatggca atgcagcatc gattgcgctg
     5761 cgcggtcatc ggaccgccgc ggcgcggccc ggcggcacgg gcgagggccg gtgtcatcgt
     5821 cagctcaacg agttgcggta gtagtgccgg tcggtgcggt acaggccgcg ccgcagctcc
     5881 accggcttgt cgccgtaggt gaaggacagc cgttcgaccg acagcagggg cgtcccgacc
     5941 ggcacgcgga gcactccggc ggtggcctcg tcggccgcga cggcgcggat cttctcctcg
     6001 gcgcggatca tgcggacgtc gaactcggtc tcgaacagcc ggtacatggg gcccacgtag
     6061 tcggtgatgc gctcggcggt caggcccttg aagaggggcg cgggcagcca gatgtcgtcc
     6121 agcaccacgg gctcgccgtc ctgcgcgagc gtgcgccgca cgagcagcgc gccctccgcg
     6181 cccttcaggt cgagcgcgcg cgcgatgggc gccggcgcgc gcatgcggcg gcagtcgagg
     6241 acatggcgcg tcatgccgcg cgggcgctgg tcgtcgggcg cgaggcgcag gaagcggtac
     6301 tggatcttct cttcggcgtg cgtggcgacg aaggtgccct tgccctggcg gcgcacgacc
     6361 aggttctcgg tggcgagctc gtcgatcgcc ttgcgcaccg tgccctggct cacgcggtag
     6421 cgctgggcga gatcggtctc ggcggggatg gcctcgccgg gcttccactc gccgtcctgc
     6481 aggctgcgca tgatgagccc cttgatctgc tggtacaggg ggctgaaggc ggggccggac
     6541 ggatccgagg acatggacgg gaaacggagg cgtgcgggct gcgggctgga tgtcgcggga
     6601 agtgcgggcc tcgcgcacgg ccgggcgcgc gcggggcgac gcgggcgacg gcgccgcggg
     6661 gcgattgcaa cacgagccgg ccgccgccgt ccaccgtggc atggatcatc ctagtgcgct
     6721 actcttgtat aagacataag atgattgacc ctcctggggt ctacccctag agtcggatta
     6781 aactcgcacc cttgcgcgcg gctcccgcgc cggcgtgcag tccggatccg aggtcccgcg
     6841 cggccctccc gccgcgccgt cgaccgcccc gtttcgccat tccctctcgt ctcggagaca
     6901 cctcaccatg agcaagaccc ccgttcgcgt cgccgtcacc ggcgccgccg gccagatcgg
     6961 ctacgccctg ctgttccgca tcgcctcggg cgagatgctg ggcaaggacc agccggtcat
     7021 cctc
//
LOCUS       DASVLN010000604         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_407500, whole genome shotgun sequence.
ACCESSION   DASVLN010000604 DASVLN010000000
VERSION     DASVLN010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407500"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..751
                     /locus_tag="VF453_14495"
     CDS             80..751
                     /locus_tag="VF453_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7688920.1"
                     /translation="MTAPRPTDPPPGAAAESALRKRPSQTRAMRTLDTLFEAATRVLA
                     SEGEAGFTTNRIAERAGFSIGTLYQYFPSKEAIIVALVRRQRGIVMRRLDVMLDRAVE
                     ERADPQAVLRAYLREITEAFGRGQKAQRLLTRLGWQLDAPALIVQAMDDGAARIRNAL
                     QRLADPTLPVPDDATLFLLTRAVIGAVRSAAVEEHAMLDDPRFEEALFRMVWGLVHRS
                     PDAAR"
     gene            835..1305
                     /locus_tag="VF453_14500"
     CDS             835..1305
                     /locus_tag="VF453_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688921.1"
                     /translation="MSLIRHLPSALAAACAAAVLSACVVAPPPPAHHPAYLHALTDLR
                     DARWNLGHRPGDAAVSQQEDVAIVEIDRAIADARAAAAEDGKNLADHPHEDARLDRRG
                     RLHRAVELLHKAHQDIAQAEDNPDTRELRNRVVGHIDAATRAAEHALHDAEQGL"
     gene            1362..2243
                     /gene="dapF"
                     /locus_tag="VF453_14505"
     CDS             1362..2243
                     /gene="dapF"
                     /locus_tag="VF453_14505"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX7688922.1"
                     /translation="MQPSPTRVRFTKMQGAGNDFVVLDATRSPLALGPEEMRRLGDRR
                     FGVGADQILVIEPAPAPGFDFAYRIFNNTGDEVEHCGNGARCFARYVRDRGLVDRDTI
                     RVKTVNNELQLDIQPDGRVRVDMNAPRFDHASLPFDAAGLAPRREGGFELWPLGLADP
                     ATGAPLEAAVLSMGNPHAVLRVEDVERFPVLEVGPRIERHPRFPRRVNTGFVEVVARD
                     HVRLRVFERDAGETLACGTGACAAVVAGIRLGWLDTRVDVDAHGGRLTVEWAGGADDH
                     VFLTGPAQTVFEGEIEL"
     gene            2240..2926
                     /locus_tag="VF453_14510"
     CDS             2240..2926
                     /locus_tag="VF453_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEX7688923.1"
                     /translation="MTINANDGITEDDIAGYLANNPGFFERHAELLASVQLSSPHGTR
                     AVSLQERQMEMLRERIKGLERKIMEMIRFGQDNMAIADRLHRWTRAVMLTHDPERLAP
                     VLLHDLQHQFMIPQAAVRIWGVAPELAHLPCAAEVSDDVKIFATSLVAPYCGANSGFE
                     ASAWFENPDSVASLALIPLRPDRQHQAFGLLALGSPDPTRYTPDMGVEFLAQVGDIAG
                     AALTRLLPAD"
     gene            2944..>3534
                     /locus_tag="VF453_14515"
     CDS             2944..>3534
                     /locus_tag="VF453_14515"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX7688924.1"
                     /translation="MSDSAPAADSPVVRYLRHLRVERRVSPHTLAAYGDALDRLEQLA
                     AGAQRPLGKLENAHVRRFAAQLHAGGLGARSIALVLSGWRGFYRWWSQQADGRMLAQN
                     PVEGVRAPKAARPLPKALAVDEAVSVMTHAEAESGEPETTSGVDDAQAAALAARDRAI
                     AELLYGCGLRLGELLGLDLRPGRADGGWVDVADGSAH"
BASE COUNT          482 a         1382 c         1240 g          430 t
ORIGIN      
        1 cgagtggggg aaccccgcgc cgggcccggc cgggggctca gttttgcggc gcgccggccg
       61 ggggccctac agtcgaggca tgaccgcgcc ccgtccgacc gatccgccgc ccggcgccgc
      121 cgccgagagc gcgctgcgca agcgcccttc gcagacgcgc gcgatgcgca cgctcgacac
      181 gctgttcgag gccgcgacgc gcgtgctcgc gagcgagggc gaggccggct tcacgaccaa
      241 ccgcatcgcc gagcgcgccg gcttctcgat cggcacgctg taccagtact tcccgagcaa
      301 ggaggcgatc atcgtcgcgc tcgtgcggcg ccaacgcggc atcgtgatgc ggcggctgga
      361 cgtgatgctc gatcgcgccg tcgaggagcg cgccgatccg caggccgtcc tgcgcgccta
      421 cctgcgcgag atcaccgagg ccttcggacg cggccagaag gcgcagcggc tgctgacgcg
      481 gctcggctgg cagctcgacg cgccggcgct gatcgtgcag gcgatggacg acggcgccgc
      541 gcgcatccgc aacgcgctcc agcgcctcgc cgacccgacg ctgccggtgc ccgacgacgc
      601 gacgctgttc ctgctcacgc gcgccgtgat cggggcggtg cgctcggcgg cggtcgagga
      661 gcacgcgatg ctcgacgacc cgcgcttcga ggaggcgctg ttccgcatgg tctggggcct
      721 cgtccaccga tcgccggacg cggcgcgatg acagggtaaa cgcccgtcgg cttgcgctca
      781 gcctggggcg ttacaacgcg aggccttccc gacaacacct cacgaaagac ctgcatgagc
      841 ctcatccgcc acctgccgtc cgcgctcgcc gcggcctgcg ccgccgccgt cctgagcgcc
      901 tgcgtcgttg cgccgccgcc tccggcccac catcccgcct acctgcacgc gctcaccgac
      961 ctgcgcgacg cgcgctggaa cctcgggcac cgccccggcg acgccgccgt gagccagcag
     1021 gaggacgtgg cgatcgtcga gatcgaccgc gccatcgccg acgcccgcgc cgcggccgcg
     1081 gaggacggca agaacctcgc cgaccatccg cacgaggacg cgcgcctgga ccgccgcggc
     1141 cgcctgcacc gcgccgtcga actgctgcac aaggcgcacc aggacatcgc gcaggccgag
     1201 gacaaccccg acacgcgcga gctgcgcaac cgcgtcgtcg gccacatcga cgccgcaacc
     1261 cgtgcggccg aacacgcgct gcacgacgcc gaacagggcc tctgaggcgc ccctcgcagc
     1321 cgcgcccccg gaggccggcc gcgaacggcg ataatcgccc gatgcagccc tccccgaccc
     1381 gcgtgcgctt caccaagatg cagggcgccg gcaacgactt cgtcgtgctc gacgcgacgc
     1441 gctcgccgct cgcgctgggc ccggaggaga tgcgccgcct cggcgaccgc cgcttcggcg
     1501 tcggcgccga ccagatcctg gtcatcgagc ccgccccggc gccgggcttc gacttcgcct
     1561 accgcatctt caacaacacc ggcgacgagg tcgagcactg cggcaacggc gcgcgctgct
     1621 tcgcgcgcta cgtgcgcgac cgcgggctcg tcgaccgcga cacgatccgc gtgaagacgg
     1681 tcaacaacga gctccagctc gacatccagc ccgacggccg cgtgcgcgtc gacatgaacg
     1741 cgccgcgctt cgaccacgcg tcgctgccgt tcgacgccgc cggcctggcg ccgcgccgcg
     1801 agggcggctt cgagctctgg ccgctgggcc tggccgatcc cgccaccggc gcaccgctcg
     1861 aggcggccgt gctgtcgatg ggcaacccgc acgcggtgct gcgcgtcgag gacgtcgagc
     1921 gcttcccggt gctcgaggtc ggcccacgga tcgagcgcca tccgcgcttc ccgcgccgcg
     1981 tcaacaccgg cttcgtcgag gtcgtcgcgc gcgaccacgt gcggctgcgc gtgttcgagc
     2041 gcgacgccgg cgagacgctc gcctgcggca cgggcgcctg cgccgcggtg gtcgcgggca
     2101 tccgcctggg ctggctggac acgcgcgtcg acgtcgacgc ccacggcggc cgcctgacgg
     2161 tcgaatgggc cggcggcgcc gacgaccacg tcttcctcac cgggcccgcg caaaccgtgt
     2221 tcgaaggaga aatcgagctg tgacgatcaa cgccaacgac ggcatcaccg aagacgacat
     2281 cgccggctac ctggccaaca accccggctt cttcgagcgc cacgccgagc tcctcgcgag
     2341 cgtgcagctc tcaagcccgc acggcacgcg cgccgtctcg ctgcaggagc ggcagatgga
     2401 aatgctgcgc gagcgcatca agggcctgga gcgcaagatc atggagatga tccgcttcgg
     2461 ccaggacaac atggcgatcg ccgaccggct gcaccgctgg acgcgcgccg tgatgctcac
     2521 gcacgacccc gagcggctcg cgcccgtgct gctgcacgac ctgcagcacc agttcatgat
     2581 cccgcaggcc gcggtgcgga tctggggcgt cgcgcccgag ctcgcccacc tgccctgcgc
     2641 cgccgaggtc agcgacgacg tcaagatctt cgcgacgagc ctggtcgcgc cgtactgcgg
     2701 cgccaactcg ggcttcgagg cctcggcctg gttcgagaac ccggactcgg tcgcctcgct
     2761 cgcgctgatc ccgctgcgcc ccgaccgcca gcaccaggcg ttcggcctgc tcgccctggg
     2821 ctcgcccgat ccgacccgct acacgccgga catgggcgtc gagttcctcg cgcaggtggg
     2881 cgacatcgca ggcgcggcgc tgacccgtct gctgcccgcg gattgaaggg ggcgagggcc
     2941 gccgtgtccg actccgcgcc ggccgccgat tcgcccgtcg tccgctacct gcggcacctg
     3001 cgcgtcgagc gccgcgtctc gccgcacacg ctggcggcgt acggcgacgc gctcgaccgc
     3061 ctggagcagc tcgccgccgg ggcgcagcgg ccgctcggca agctcgagaa cgcgcacgtg
     3121 cgccgcttcg ccgcgcagct gcacgcgggc gggctgggcg cccgctcgat cgcgctggtg
     3181 ctctcgggct ggcgcggctt ctaccgctgg tggagccagc aggccgacgg gcgcatgctg
     3241 gcgcagaacc cggtcgaggg cgtgcgtgcg cccaaggccg cgcggccgct gcccaaggcg
     3301 ctggccgtcg acgaggccgt cagcgtgatg acgcatgccg aggccgagtc cggcgagccg
     3361 gagacgacgt ccggcgtcga cgacgcccag gccgcggcgc tcgccgcgcg ggaccgtgcg
     3421 atcgccgagc tgctgtacgg atgcggcctg cgcctgggcg agctgctcgg gctggacctg
     3481 cggccgggcc gcgccgacgg cggctgggtc gacgtcgccg acggcagcgc ccac
//
LOCUS       DASVLN010000605         3372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_517309, whole genome shotgun sequence.
ACCESSION   DASVLN010000605 DASVLN010000000
VERSION     DASVLN010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517309"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1471)
                     /gene="creD"
                     /locus_tag="VF453_14520"
     CDS             complement(32..1471)
                     /gene="creD"
                     /locus_tag="VF453_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEX7688925.1"
                     /translation="MRFPVMAKLGALAGVGLVLCGVLARIDGLVDERGQRREEAVRGV
                     EQAHAGAQAGLGPLLVRRCTERWDEAASKAEREPAATERSFLLRQGPAQLTVDADTRA
                     EPLYRGLFKVNAWSGHLVLDARFDDRAALRPVATHPGGRVQCDGPQAMLALSDVRGIR
                     MAQAQLDGAPLQVRPGTPSDKYPRGLQAALPAGVVDGDGGRGPVTVRVALDLVGTGGL
                     AWVPAADQLGWTLRSDWPHPSFGGQFVPTQREVRADGFRATWALSSLATTAVADVGRD
                     VGLCVPAGAGAGGGEAAPKGDNCLDTMAVAFIDPVNPYVLSDRATKYALLFIVLTFVA
                     VGLVEVLSGRRVHPVQYALVGLALSLFFLLLLSLSEHLPFATAYALAATACALLLAFY
                     ASAMLGRRAAGAGFGAGIAALYGLLYALLQMEQNALVIGSVMLFAALAAVMALTRRVD
                     WYALFERMRAEPPAVASASMSEPAAGSAR"
     gene            complement(1606..3072)
                     /gene="creC"
                     /locus_tag="VF453_14525"
     CDS             complement(1606..3072)
                     /gene="creC"
                     /locus_tag="VF453_14525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HEX7688926.1"
                     /translation="MRLGLRLLFAFFVVTGLAGFFALQVFQQEVAPSVREVMEDVLVD
                     TANLLAEQAAADLRAMPPGGTLAGSRLERDIAAYRRRPIDVRIWGLRKTSLDFRVYVT
                     DATGRVVLDSGAGAPAAEGAPGGAVGADYSRWRDVSRTLHGDYGARSTREVEADSRTA
                     VMYVAAPVRDGGRVIGVVTVGEPIATVQPFIDRAQRRLLVASLWLLGASAAVGVAVTL
                     WMVVSVRKLKAYATRVHAGAREPVPQLPGELGDLAQAVDDMRARLEGRERLEHTVRAL
                     THELKSPLTAIQGAAELLAEALPEADRERFALQIQEQSLRLRDLVDRMLALSRLEGLR
                     AIEHPQPVALLALVDRVVAEGAVLAAQRGVRAQWAARDDTTLRAADPESLGLLFSNLW
                     ANALDFAPEGSPVTLSLRREGREAVFELCDAGPGVSDFALPQLGQRFFSLPRPRDGRR
                     GSGLGLAIVRQAAALHGGSVAFEPADPGLRVVVRLPLG"
     gene            complement(3085..>3372)
                     /locus_tag="VF453_14530"
     CDS             complement(3085..>3372)
                     /locus_tag="VF453_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7688927.1"
                     /translation="ELDADGQRIRFAGEWLALTRLEFGLLSALLRAPGRIRSRAALLD
                     EVWGADGEATDRTVDTHVKTLRAKLRAVRPELEPIATHRGMGYSLELPPRT"
BASE COUNT          465 a         1309 c         1239 g          359 t
ORIGIN      
        1 ggccggtcgc gttcaggcat cgcggccgcc ctcatcgcgc gctcccggcc gcaggctcgg
       61 acatcgaggc cgacgcgacg gcgggcggct cggcgcgcat ccgctcgaac agcgcgtacc
      121 aatcgacccg acgcgtgagc gccatgacgg ccgccagcgc cgcgaacagc atgaccgagc
      181 cgatcacgag cgcgttctgc tccatctgca gcagcgcgta cagcagcccg tacagcgccg
      241 cgatgcccgc gccgaagccg gcaccggccg cgcgccggcc cagcatggcg ctcgcgtaga
      301 aggccagcag cagcgcgcag gccgtggcgg cgagggcgta ggcggtcgcg aagggcaggt
      361 gctcggacag cgacagcagc agcaggaaga acaggctcag cgccaggccg accagcgcgt
      421 actgcaccgg atgcacgcgc cggcccgaca gcacctcgac cagcccgacc gcgacgaagg
      481 tcagcacgat gaacagcagc gcgtacttgg tcgcgcggtc gctcagaacg taggggttga
      541 ccgggtcgat gaaggcgacc gccatcgtgt ccaggcagtt gtcgcccttg ggcgccgcct
      601 cgccgcctcc ggcgcccgcg cccgcaggca cgcacaggcc gacgtcgcgg ccgacgtcgg
      661 cgacggcggt cgtggcgagc gaggacaacg cccacgtcgc ccggaagccg tcggcgcgca
      721 cctcgcgctg cgtcgggacg aactggccgc cgaaggacgg gtgcggccag tcggagcgca
      781 gcgtccagcc cagctggtcg gccgcgggca cccaggcgag gccgccggtg cccaccagat
      841 ccagcgccac gcgcaccgtc accggccccc ggccgccgtc gccgtcgacc acgcccgccg
      901 gcaaggccgc ctgcaggccg cgagggtact tgtcgctggg cgtgccgggc cgcacctgga
      961 gcggtgcgcc gtcgagctgc gcctgcgcca tgcggatccc ccgcacgtcc gacagcgcca
     1021 gcatcgcctg cggtccatcg cactggacgc gcccccccgg atgcgtcgcg acggggcgca
     1081 gcgcggcgcg gtcgtcgaag cgcgcgtcca gcacgaggtg gcccgaccag gcgttgacct
     1141 tgaacaggcc gcggtacagc ggctcggcgc gcgtgtccgc atcgaccgtg agctgcgcgg
     1201 gcccctgccg cagcaggaag ctgcgctcgg tcgccgcggg ctcgcgctcg gccttcgatg
     1261 ccgcctcgtc ccagcgttcg gtgcagcgcc gcacgagcag gggcccgagc ccggcctgcg
     1321 cgcccgcgtg cgcctgctcg acgccgcgca ccgcctcttc gcggcgctgc ccgcgctcgt
     1381 cgaccagccc gtcgatccgc gccagcacgc cgcacagcac caggcccacc ccggccagcg
     1441 cgccgagctt ggccatcacg ggaaacctca tcgcatcctc cctccgcgcc gggacgccgg
     1501 cgcgcacggc cgtgacgatg cgggcggtgc gtgaggggac gatggcgtcc gtgaagcggg
     1561 cgtgaagcgc ggcgcgtccg gcctcccgcg ggttcgtcca caggttcagc cgagcggcag
     1621 ccgcacgacc acccgcagcc ccggatcggc gggctcgaac gccacgctgc cgccatgcag
     1681 cgctgccgcc tggcgcacga tggccaggcc cagtccgctg ccgcggcgac cgtcgcgcgg
     1741 gcgcggcagc gagaagaagc gctggccgag ctgcggcagc gcgaagtcgg agacgcccgg
     1801 cccggcgtcg cacagctcga agacggcctc gcgcccctcg cgccgcagcg agagcgtgac
     1861 cggcgagcct tcgggcgcga agtcgagcgc gttcgcccac aggttggaga acagcagccc
     1921 gagcgactcc ggatcggcgg cgcgcagggt cgtgtcgtcg cgggccgccc actgcgcccg
     1981 cacgccacgc tgcgccgcga gcaccgcgcc ctcggcgacg acgcggtcga cgagcgccag
     2041 cagcgccacc ggctgcggat gctcgatcgc gcgcaggcct tcgagccgcg acagcgcgag
     2101 catgcggtcg accaggtcgc gcaggcgcag cgactgctcc tggatctgca gcgcgaagcg
     2161 ctcgcggtcg gcctcgggca gcgcctcggc gagcagctcc gcggcgccct ggatcgcggt
     2221 gagcgggctc ttgagctcgt gggtcagcgc gcgcacggtg tgctccaggc gttcgcggcc
     2281 ctccagccgc gcgcgcatgt cgtcgaccgc ctgggcgagg tcgccgagct cacccggcag
     2341 ctggggcacc ggctcgcgcg cgccggcgtg cacgcgcgtc gcgtaggcct tgagcttgcg
     2401 caccgagacc accatccaca gcgtcaccgc gacccccacc gcggcggacg cgcccagcag
     2461 ccagaggctc gccaccagca gccggcgctg ggcgcggtcg atgaagggct gcacggtggc
     2521 gatcggctcg ccgaccgtca cgacgccgat cacccggccg ccgtcgcgca cgggtgccgc
     2581 cacgtacatc accgcggtgc gcgagtcggc ctcgacctca cgcgtcgagc gcgccccgta
     2641 gtcgccgtgc agcgtgcggc tgacgtcgcg ccagcgggaa tagtcggcgc cgacggcgcc
     2701 gcccggcgcg ccttccgccg cgggtgcgcc ggcgccggaa tcgagcacca cgcggcccgt
     2761 ggcatcggtc acgtagacgc ggaagtcgag cgaggtcttg cgcagccccc agatgcgcac
     2821 gtcgatcggc cgccggcggt aggcggcgat gtcgcgctcc agccgggaac ccgccagcgt
     2881 ccccccgggc ggcatcgcgc gcaggtcggc ggcggcctgc tcggcgagca ggttggcggt
     2941 gtcgacgagc acgtcctcca tcacctcgcg cacgctcggc gcgacctcct gctggaagac
     3001 ctggagcgcg aagaagccgg ccagcccggt gacgacgaag aacgcgaaca gcaggcgcag
     3061 gccgaggcgc atggctcgcg cttctcaggt ccggggcggc agctcgagcg agtagcccat
     3121 gccgcggtgc gtcgcgatcg gctcgagctc gggccgcacg gcgcgcagct tggcgcgcag
     3181 cgtcttcacg tgggtgtcga cggtgcggtc cgtcgcctcg ccgtcggcgc cccagacctc
     3241 gtcgagcagc gccgcgcgcg agcggatgcg ccccggcgcc cgcaggagcg cgctcagcag
     3301 gccgaactcg agccgcgtga gcgcgagcca ctcgccggcg aagcggatgc gctggccgtc
     3361 ggcgtccagc tc
//
LOCUS       DASVLN010000606         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_407622, whole genome shotgun sequence.
ACCESSION   DASVLN010000606 DASVLN010000000
VERSION     DASVLN010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407622"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="VF453_14535"
     CDS             complement(<1..1977)
                     /locus_tag="VF453_14535"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7688928.1"
                     /translation="MPPPNIFQRPAPRKPAPSDPLAMLEVRQAMLLHQAGKVAEAATV
                     YERVLARDPAQFESRHYLGVAQLQSGRPGEALATLERALALRADDASLQSNVGLALMT
                     LGRHAEALPRFDRALALQPGMPEALNNRGWTLRHLDRLEDALADLDRALAARPNFAEV
                     AHRRALVLGALQRHDEAVRAFEQAIALRPRDAEAHGNLGTLLTLMGRGAEARAALAKA
                     AALAPEAMVHRLKHAVAWLPLVRMAGDDLDAARAAFTREVEALLAEARAGTVARPEQV
                     VGAAQPFYIAYQERGNVAPLSAYGDFCAALMARWLAGRDVAGATPDTGSAQTPRRVRV
                     GIVSSQVFSHSVWQANVRGWVEELDRDRFEVHVFHLGRTHDAETAIAQARADGFVSGE
                     RSLGEWVDAIRAARADVLLYPELGIDALTPRLAAMRLAPVQAASWGHPETTGLPTIDH
                     YLSAELFEDAGSDAHYRESLVRLPGLGCCVAPAAIRPAQQAVEVAFADLGLAPGASVF
                     VCAGTPFKYLPEHDAVLVRIAQALPSAQFLFFNHGLAASLSAQLAARLAAAFDAAGLD
                     PARHLVAAPWMERARFFGVLSQADAFLDTIGFSGFNTVMQAVECGLPVVTRRGGFMRG
                     RLGSGILERMGLADQVAHDDAAYVALALRLAQDAD"
     gene            complement(2057..3040)
                     /locus_tag="VF453_14540"
     CDS             complement(2057..3040)
                     /locus_tag="VF453_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX7688929.1"
                     /translation="MTKAIQITEFGGPEQLRLVDVEVGDPGPGQVRVRHRAIGINFID
                     VYQRTGLYPNPLPLTPGGEGAGVVEAVGEGVTHLAVGDRVAYAAPKAGSYCEARVLDA
                     THVCRLPDDIDFETGAAMMLKGLTANYLLRRTLPVEGLAPGDHVLFHAAAGGVGLIAC
                     QWARALGYELIGTAGSPEKCRLALDNGAAHAIDYSREDFVARVREITGGKGVKVVYDS
                     VGKDTFERSIDCLRPFGLLAVFGNSSGPAGPVPLQMLAAKGSLYVTRPTLFTHTSTRE
                     RTQAMADELFQVVRDGSVKIRIDQRYPLAEAERAHRDLEGRRTTGSSVLLV"
     gene            complement(3160..>3467)
                     /locus_tag="VF453_14545"
     CDS             complement(3160..>3467)
                     /locus_tag="VF453_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX7688930.1"
                     /translation="RTSPPAAFATTFVLTIANPSTILSFVAVIAALGVSGPRAAGAAW
                     WLVAGVFAGSAAWWLILVGAVHFARRALPLRALRWIDLATAIVLLGMAAWAAARVLA"
BASE COUNT          478 a         1279 c         1300 g          410 t
ORIGIN      
        1 gtccgcgtcc tgcgcgaggc ggagcgccag cgcgacgtag gcggcgtcgt cgtgcgccac
       61 ctggtcggcc aggcccatgc gctcgaggat gccgctgccc aggcgcccgc gcatgaaccc
      121 gccgcggcgc gtgacgaccg gcaggccgca ttcgaccgcc tgcatcaccg tgttgaagcc
      181 ggagaagccg atcgtgtcga ggaaggcatc ggcctgcgaa agcacgccga agaagcgcgc
      241 gcgctccatc cacggggcgg ccacgaggtg ccgcgccgga tccaggcccg cggcgtcgaa
      301 ggccgccgcg agccgcgccg ccagctgcgc ggacagcgag gccgcgaggc cgtgattgaa
      361 gaacaggaac tgcgccgagg gcagcgcctg ggcgatgcgc accagcaccg cgtcgtgctc
      421 cggcaggtac ttgaagggcg tgccggcgca gacgaagacg ctcgcccccg gcgcgaggcc
      481 gaggtcggcg aacgcgacct ccacggcctg ctgcgcgggc cgtatcgccg ccggcgcgac
      541 gcagcacccc aggcctggca ggcgcacgag cgattcgcgg tagtgcgcat cggagccggc
      601 atcctcgaac agctcggccg acaggtagtg gtcgatggtg ggcagcccgg tggtctcggg
      661 atggccccag ctggccgcct gcaccggcgc gagccgcatc gccgccagcc gcggcgtgag
      721 cgcgtcgatg cccagctcgg gatagagcag cacgtcggcg cgcgcggcgc ggatcgcgtc
      781 gacccactcg cccaggctgc gctcgccgct cacgaagccg tcggcgcgcg cctgcgcgat
      841 cgcggtctcg gcgtcgtgcg tgcggccgag gtggaagacg tgcacctcga agcgatcgcg
      901 atcgagctcc tcgacccagc cgcgcacgtt ggcctgccag accgagtgcg agaacacctg
      961 cgaggacacg atgcccacgc gcacgcgtcg cggcgtctgc gcagaaccgg tgtccggcgt
     1021 cgcgcccgcg acgtcgcggc cggccagcca acgcgccatc agtgcggcgc agaagtcgcc
     1081 gtacgccgac agcggcgcga cgttgccgcg ctcctggtag gcgatgtaga acggctgcgc
     1141 ggcgccgacg acctgctccg gccgcgccac cgtgcccgcg cgcgcctcgg cgagcagcgc
     1201 ctcgacctcg cgcgtgaatg cagcgcgcgc tgcgtcgagg tcgtcgcccg ccatgcgcac
     1261 cagcggcagc caggcgacgg cgtgcttcag tcgatgcacc atcgcttcgg gcgcgagcgc
     1321 cgcggccttg gcgagagcgg cacgcgcctc ggcgccgcgg cccatcagcg tgagcagggt
     1381 gcccaggttg ccgtgcgcct cggcgtcgcg cggacgcagc gcgatggcct gctcgaacgc
     1441 gcgcacggcc tcgtcatggc gctgcagcgc gcccagcacc agcgcgcgcc ggtgtgcgac
     1501 ctccgcgaag ttcgggcgcg ccgcgagcgc ccggtcgagg tcggccagcg cgtcctcgag
     1561 gcggtcgagg tggcgcagcg tccagccgcg gttgttcagg gcctcgggca tgccgggctg
     1621 caacgcgagc gcgcggtcga agcgcggcag cgcctcggcg tggcggccca gggtcatcag
     1681 cgcgagcccg acgttggact gcagcgaggc gtcgtccgcg cgcagggcca gcgcgcgctc
     1741 cagcgtcgcc agagcctccc ccggccggcc cgactgcagc tgcgcgacgc cgaggtagtg
     1801 gcgcgactcg aattgcgccg gatcccgcgc cagcacgcgc tcgtagaccg tggccgcctc
     1861 ggcgaccttg ccggcctggt gcagcagcat cgcctggcgc acctccagca tcgcgagcgg
     1921 gtcggacggc gccggcttgc gcggcgcggg gcgctggaag atgttgggcg gcggcatggc
     1981 gtctcgatcg tggcggagac gccgattatc cagtcgaggc cgcccgccgc ggcggtgcgc
     2041 gggtcgaggc cgggatctag acgagcagca ccgacgagcc ggtcgtgcgc cggccctcga
     2101 ggtcgcggtg cgcgcgctcc gcctcggcga gcgggtagcg ctggtcgatg cggatcttga
     2161 cgctgccgtc gcgcacgacc tggaacagct cgtcggccat cgcctgcgtc cgctcgcgcg
     2221 tggaggtgtg cgtgaagagg gtcggccgcg tcacgtacag cgagcccttg gccgccagca
     2281 tctgcagcgg caccggcccc gccggacccg acgagttgcc gaacaccgcc agcagcccga
     2341 acgggcgcag gcagtcgatc gagcgctcga aggtgtcctt gccgaccgag tcgtagacca
     2401 ccttcacgcc cttgccgccc gtgatctccc gcacgcgggc gacgaagtcc tcgcgcgagt
     2461 agtcgatcgc gtgcgccgcg ccgttgtcca gcgcgagccg gcacttctcg ggcgagcccg
     2521 cggtgccgat cagctcgtag ccgagcgccc gcgcccattg gcaggcgatc aggccgaccc
     2581 cgcccgcggc cgcgtggaac aggacgtggt cgccgggcgc gaggccctcg acgggcagcg
     2641 tgcggcgcag caggtagttc gccgtcagcc ccttgagcat catggcggcg ccggtctcga
     2701 agtcgatgtc gtcgggcagc ctgcacacgt gggtcgcgtc gagcacgcgg gcctcgcagt
     2761 agctcccggc cttgggcgcg gcgtaggcga cgcggtcgcc gacggccagg tgcgtgacgc
     2821 cctcgcccac cgcctcgacg acgcccgcgc cctcgccgcc cggcgtcagc ggcagcgggt
     2881 tcgggtacag gccggtgcgc tggtagacgt cgatgaagtt gatgccgatc gcacgatgcc
     2941 gcactcgtac ctggcccggg ccgggatcgc cgacctcgac gtcgacgagg cgcagctgct
     3001 cggggccgcc gaattcggtg atctggatcg ccttggtcac gctcgttctc cggttggggc
     3061 ggcgcgcggg aaaggcggcg acgccaggcc cgggttttta gcacgcaggg ccggcccttg
     3121 ccggcgcgag gattcgtcgc accggcggac gacccgacct caggcaagca ctcgcgcggc
     3181 ggcccaggcg gccatgccca gcagcacgat cgcggtcgcg aggtcgatcc agcgcagcgc
     3241 ccgcagcggc agggcgcggc gcgcgaagtg cacggccccc acgaggatca gccaccaagc
     3301 ggccgagccc gcgaacacgc ccgccaccag ccaccacgcc gcaccggccg cgcgcgggcc
     3361 ggacaccccg agcgcggcga tcaccgcgac gaagctcagg atggtcgacg ggttcgcgat
     3421 cgtgagcacg aacgtcgtgg cgaacgcggc cggcggcgag gtgcgcg
//
LOCUS       DASVLN010000607         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_193013, whole genome shotgun sequence.
ACCESSION   DASVLN010000607 DASVLN010000000
VERSION     DASVLN010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193013"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1532
                     /locus_tag="VF453_14550"
     CDS             300..1532
                     /locus_tag="VF453_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEX7688931.1"
                     /translation="MTQHDPLSLHVPEPTGRPGCATDFSYLHVSPAGEVRRPPVDTDP
                     RSTNDLAFELIRVLDTEGNAVGPWAPDISPELMRKGLKAMMKTRIFDARMLIGQRQKK
                     MSFYMQSLGEEAIGTAHALALEDGDMCFPTYRQQSLLIVRDYPLVDMVCQLLSNERDP
                     LKGRQLPVMYSVKAKGFFSISGNLATQYIQAVGWAMASAIKNDTKIASAWIGDGATAE
                     SDFNTALTFAHVYNAPVILNVVNNQWAISTFQAIAGGEHTTFAARGVGSGIASLRVDG
                     NDFLAVYAASRWAAERARSNLGPTLIEWVTYRAGPHSTSDDPSKYRPADDWTHFPLGD
                     PIARLRQHLTKLGHWSDAEHEETHKALEAEVAAAQKEAESYGTLNDGRIPSAASMFED
                     VYKDMPEHLRRQRQELGV"
     gene            1540..2595
                     /locus_tag="VF453_14555"
     CDS             1540..2595
                     /locus_tag="VF453_14555"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX7688932.1"
                     /translation="MQTKNQLAAAPEGPTSPMTMIQALRSAMDIMLERDPNVVIFGQD
                     VGYFGGVFRCTEGLQKKYGKSRVFDAPISEGGIVGAAVGMGAYGLRPVVEIQFADYFY
                     PASDQIVSEAARLRYRSAGDFTAPLTIRMPCGGGIYGGQTHSQSPEALFTHVCGLRTV
                     MPSNPYDAKGLLISCIENDDPVIFLEPKRLYNGPFDGHHDKPVVPWSKHRLGEVPEGY
                     YTVPLDSAATYREGKDLTVLAYGTMVFVAEAAAAETGIDAEIIDLRCIWPLDLDAIVR
                     SVKKTGRCVVVHEATKTSGFGAELVALVQEHCFYHLEAPIERVAGWDTPYPHAQEWAY
                     FPGPDRVGAAFKRAMEK"
     gene            2600..>2968
                     /locus_tag="VF453_14560"
     CDS             2600..>2968
                     /locus_tag="VF453_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7688933.1"
                     /translation="MGIHIIKMPDIGEGIAEVELVAWNVKEGDTVVEDQNLADVMTDK
                     ATVEIPSPVHGKVLALGGKVGQVMAVGSELIRIEVEGAGNVKEGAAAPAPAAAAVVAA
                     PVPAPVSAPVAAKSAPAPAPA"
BASE COUNT          487 a         1082 c          992 g          407 t
ORIGIN      
        1 ggccgatccg gcgaccggcc ggcccctgca ggcgctcgcc ctcgcctgcc cgcgctgagc
       61 cgcggcgcga tgctgcaccg cgtcatcgcg gcatgcggcg gcggcgcagg gatttcgtga
      121 acccgtcgac gcggcgggcg cgcgaaatcg ggtatggtcc atgacgctgg aagcgcgcgc
      181 ggcgaccccg cgcggcgtct cccgtcccac cggccggcgc gcggcacacc ccctgcgacg
      241 ccggccgatc cgactgccaa cctttgaagc ccgccaccgc gggcgcggag ctttcgccga
      301 tgacccagca cgaccccctg agtctgcacg tcccggagcc cacggggcga ccgggctgcg
      361 cgaccgactt ctcctacctt cacgtctcgc ccgccggcga ggtccgccgc cccccggtgg
      421 acaccgatcc ccgctccacc aacgacctcg ccttcgagct gatccgcgtc ctcgacacgg
      481 agggcaacgc cgtcggcccc tgggcgccgg acatctcgcc cgagctgatg cgcaagggcc
      541 tgaaggcgat gatgaagacg cgcatcttcg acgcccgcat gctgatcggg cagcgccaga
      601 agaagatgtc gttctacatg cagagcctgg gcgaggaggc catcggcacc gcgcatgcgc
      661 tggcgctcga ggatggcgac atgtgcttcc cgacctaccg ccagcagagc ctgctgatcg
      721 tgcgcgacta cccgctggtc gacatggtct gccagctgct gtccaacgag cgcgacccgc
      781 tcaagggccg ccagctgccg gtcatgtact cggtcaaggc caagggcttc ttctcgatct
      841 ccggcaacct cgcgacgcag tacatccagg cggtcggctg ggcgatggcc tcggcgatca
      901 agaacgacac caagatcgcg tcggcgtgga tcggcgacgg cgccaccgcg gaatccgact
      961 tcaacacggc gctcaccttc gcccacgtct acaacgcgcc ggtgatcctg aacgtcgtca
     1021 acaaccagtg ggcgatctcg acgttccagg ccatcgccgg cggcgagcac acgaccttcg
     1081 cggcgcgcgg cgtcggctcc ggcatcgcgt cgctgcgcgt ggacggcaac gacttcctcg
     1141 ccgtctacgc cgcctcccgc tgggccgcgg agcgtgcgcg cagcaacctc ggtccgacgc
     1201 tgatcgagtg ggtcacctac cgcgccggcc cgcactcgac ctccgacgat ccgagcaagt
     1261 accgtcccgc ggacgactgg acgcacttcc cgctcggcga ccccatcgcg cgcctgcgcc
     1321 agcacctcac gaagctcggc cactggtcgg acgcggagca cgaggagacg cacaaggccc
     1381 tcgaggccga ggtcgccgcc gcgcagaagg aggccgagtc ctacggcacg ctcaacgacg
     1441 ggcgcatccc gagcgcggcc tcgatgttcg aggacgtcta caaggacatg ccggaacacc
     1501 tgcgccgcca gcgccaggaa ctaggggtct gaaatggaaa tgcaaaccaa gaaccagctg
     1561 gccgccgccc cggagggtcc gaccagcccg atgacgatga tccaggcgct gcgctcggcg
     1621 atggacatca tgctggagcg cgaccccaac gtcgtgatct tcggccagga cgtgggctac
     1681 ttcggcggcg tcttccgctg caccgagggg ctgcagaaga agtatggcaa gtcgcgggtg
     1741 ttcgacgcgc cgatctcgga aggcggcatc gtcggcgcgg ccgtcggcat gggcgcctac
     1801 gggctgcggc cggtggtcga gatccagttc gccgactact tctacccggc ctccgaccag
     1861 atcgtctccg aggccgcgcg cctgcgctac cgctcggcgg gcgatttcac ggcgccgctg
     1921 acgatccgca tgccttgcgg cggcggcatc tacggcggcc agacgcacag ccagtcgccc
     1981 gaggcgctct tcacgcacgt ctgcggcctg cgcacggtca tgccgtccaa cccgtacgac
     2041 gccaagggcc tgctgatctc gtgcatcgag aacgacgacc cggtgatctt cctcgagccg
     2101 aagcggctgt acaacggccc gttcgacggc caccacgaca agccggtcgt gccctggtcc
     2161 aagcaccgcc tgggcgaggt tcccgagggc tactacacgg tgccgctgga ctccgcggcc
     2221 acctaccgcg agggcaagga cctcacggtg ctcgcgtacg gcacgatggt cttcgtcgcc
     2281 gaggccgcgg ccgcggagac cggcatcgac gccgagatca tcgacctgcg ctgcatctgg
     2341 ccgctggatc tcgacgcgat cgtgcggtcc gtgaagaaga cgggccgctg cgtcgtcgtc
     2401 cacgaggcga cgaagacgag cggcttcggc gcggagctcg tcgcgctggt gcaggagcac
     2461 tgcttctacc acctggaggc gcccatcgag cgcgtggcgg gctgggatac cccctacccg
     2521 catgcgcagg agtgggcgta tttcccgggg cccgaccgcg tcggcgccgc gttcaagcga
     2581 gcgatggaga aataagaaca tgggaatcca catcatcaag atgcccgaca tcggcgaagg
     2641 catcgcggaa gtcgagctgg tcgcctggaa cgtcaaggag ggcgacacgg tcgtcgagga
     2701 ccagaacctc gccgacgtca tgacggacaa ggccaccgtc gagatcccct cgcccgtgca
     2761 cggcaaggtg ctcgcgctgg gcggcaaggt gggccaggtg atggcggtcg gctcggagct
     2821 gatccgcatc gaggtcgagg gcgcgggcaa cgtcaaggag ggcgcggccg cgcccgcacc
     2881 ggccgccgcg gcggtcgtcg cggcacccgt acccgctccg gtatccgcgc ccgtcgccgc
     2941 gaagtccgca ccggcgcccg cccctgcg
//
LOCUS       DASVLN010000608         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_31930, whole genome shotgun sequence.
ACCESSION   DASVLN010000608 DASVLN010000000
VERSION     DASVLN010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31930"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VF453_14565"
     CDS             complement(<1..1022)
                     /locus_tag="VF453_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEX7688934.1"
                     /translation="MFKNAIVYRIAHWDEPTLPALEERLATLRFVECGASQPESAGWV
                     EPRGEKHGALVESVGGQWILKLCTETKAVPGGAVKQLLEARCDQIEQETGRRPKGKRA
                     KELKEEIVQELLPRAFPKRSTTPVWIDLKAGLLMVGAGSAKKADKVTTLLGELLAGGL
                     RLELVQTQMSAAAAMAEWLSTRQPPAGFTIDRECELKQPDSEKSLVRYARHTLDIDEV
                     GEHIRQGKLPTSLAMTWADRVSFVLTENFALKKIKLLDVVLEGQPQGGKADENFDADV
                     AITTGELAQLIPELVEALGGPLELGAAAAPTTSDKVIALLTGQPAEGEASVYAPPRAE
                     DASAPF"
     gene            1145..1675
                     /gene="mobB"
                     /locus_tag="VF453_14570"
     CDS             1145..1675
                     /gene="mobB"
                     /locus_tag="VF453_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX7688935.1"
                     /translation="MKVLGIAGSSGMGKTTLLARLVPALGARGRVVSMIKHSHKDIEV
                     DRPGKDSWRLREAGCREVLLLGRTRWALMHELRGAPEPALDEMLARIGPCDLVLVEGF
                     KAGDFPKLEVWRAEPGKPLLWPRWPGIRAIASDDDPPIRDAASADEPPPVHLRLADTE
                     AIADFVLAHAVDVART"
     gene            1678..2511
                     /gene="hyi"
                     /locus_tag="VF453_14575"
     CDS             1678..2511
                     /gene="hyi"
                     /locus_tag="VF453_14575"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HEX7688936.1"
                     /translation="MLRFAANVSMLFAEAPFLDRFGLAARAGFAGVECQFPYEAPAAA
                     IRARLDEHGLAMVLHNLPAGDWAAGDRGIACQPARRAEFREGVARAIDYARTFGVPQL
                     NCLAGIAPVDADPAEVHATLVDNLRFAARALADAGLRLLVEPINTFDIPGFAVNRTDQ
                     AIALLDDVDAPNAFLQYDVYHAQRMEGELCGTLARHLPRIAHVQVADNPGRHEPGTGE
                     IRFGFVFAELERLGYGGWIGCEYRPAGRTDAGLDWLPRGALRPGGQTARQKPPGLAAG
                     G"
BASE COUNT          343 a         1005 c          942 g          379 t
ORIGIN      
        1 cagaacggcg ccgacgcgtc ctcggcccgc ggcggcgcgt agaccgacgc ctcgccctcg
       61 gcgggctggc cggtcagcag cgcgatcacc ttgtcggacg tggtcggcgc cgcggccgcg
      121 cccagctcga gcggcccgcc cagcgcctcg acgagctcgg ggatcagctg cgccagctcg
      181 ccggtcgtga tcgcgacgtc ggcgtcgaag ttctcgtcgg ccttgccgcc ctggggctgt
      241 ccttcgagga ccacatccag cagcttgatc ttcttcagcg cgaagttctc ggtcagcacg
      301 aacgagacgc ggtcggccca ggtcatcgcg agcgaggtcg gcagcttgcc ctggcggatg
      361 tgctcgccga cctcgtcgat gtccagcgtg tggcgggcgt agcgcacgag cgacttctcg
      421 ctgtcgggct gcttgagctc gcactcgcgg tcgatcgtga agccggcagg cggctggcgg
      481 gtcgacagcc attcggccat cgccgcggct gcggacatct gcgtctgcac gagctccagc
      541 cgcaggccgc cggcgagcag ctcgcccagc agcgtcgtga ccttgtccgc cttcttcgcg
      601 ctgcccgcgc cgaccatcag cagcccggcc ttcaggtcga tccacacggg cgtcgtcgag
      661 cgcttgggga acgcgcgcgg cagcagctcc tgcacgatct cttccttcag ctccttggcg
      721 cgcttgccct tggggcggcg acccgtctcc tgctcgatct ggtcgcagcg cgcctccagc
      781 agctgcttga cggcgccgcc gggcacggcc ttggtctcgg tgcagagctt gaggatccac
      841 tgcccgccga cgctctcgac cagcgcgccg tgcttctcgc cgcgcggctc gacccagccg
      901 gcggactcgg gctgcgacgc gccgcactcg acgaagcgca gcgtggccag gcgctcctcg
      961 agcgcgggca gcgtgggctc gtcccagtgg gcgatgcggt agacgatggc gttcttgaac
     1021 atgcgcgaaa acttcttcga cggacggagc gcgcgattct aggcgcactg gatttccgtc
     1081 cagcccggca ggactcgcgc ggcggcgcgg cggcggcatt cctgcacgcg cgagaatgcg
     1141 cgccatgaaa gtcctcggca tcgccggctc ctcgggcatg ggcaagacga cgctgctcgc
     1201 acggctggtg ccggcgctgg gcgcgcgcgg gcgggtggtc tcgatgatca agcacagcca
     1261 caaggacatc gaggtcgacc gccccggcaa ggactcgtgg cggctgcgcg aggccggctg
     1321 ccgcgaggtg cttctgctcg ggcgcacccg ctgggcgctg atgcacgagc tgcgcggcgc
     1381 gcccgagccg gcgctggacg agatgctcgc ccgcatcggg ccgtgcgacc tcgtgctggt
     1441 ggagggcttc aaggcgggcg atttccccaa gctcgaggtc tggcgcgccg agcccggcaa
     1501 gccgctcctg tggccgcggt ggcccggcat ccgcgcgatc gcgagcgacg acgacccgcc
     1561 catacgggat gccgcatcgg ccgacgagcc gccgcccgtg cacctgcgcc tcgccgacac
     1621 cgaggccatc gccgacttcg tgctcgcgca cgcggtggac gtcgcgagga cctgaccatg
     1681 ctgcgcttcg ccgccaacgt ctcgatgctg ttcgccgagg cgcccttcct cgaccgcttc
     1741 ggcctcgccg cgcgcgcggg cttcgcgggc gtcgagtgcc agttccccta cgaggcgccg
     1801 gcggcggcga tccgcgcacg cctggacgag cacggcctcg cgatggtgct gcacaacctg
     1861 cccgcgggcg actgggccgc cggcgaccgc ggcatcgcgt gccagcccgc ccgccgcgcc
     1921 gagttccgcg agggcgtcgc ccgcgcgatc gactacgcgc gcacgttcgg cgttccgcag
     1981 ctgaactgcc tggccggcat cgcgcccgtc gacgcggatc ccgccgaggt gcacgccacg
     2041 ctcgtcgaca acctgcgctt cgcggcacgg gcgctcgccg atgccggcct gcggctgctc
     2101 gtcgagccga tcaacacctt cgacatccct ggcttcgcgg tgaaccgcac ggaccaggcg
     2161 atcgcgctgc tcgacgacgt cgacgccccc aacgcgttcc tgcagtacga cgtctaccac
     2221 gcgcagcgca tggagggcga gctgtgcggc accctcgcgc gccacctgcc gcgcatcgcg
     2281 cacgtgcagg tcgccgacaa ccccggtcgc cacgagccgg gcacgggcga gatccgcttc
     2341 ggcttcgtgt tcgccgagct cgagcgcctg ggctacggcg gctggatcgg ctgcgaatac
     2401 cggcccgcgg gacgcaccga cgccgggctc gactggctgc cgcgcggcgc gctgcggccc
     2461 ggcgggcaaa ctgcgcggca gaagccaccc ggcctcgcag ccggcggctg aacgcaggcc
     2521 ctaggcttcc tcgaaacgct tgcgcacgaa cgcccacgcg agccgcgagg cgtcgggacc
     2581 ctcgggatcg ccgaacggct cgcgggtggc gccgccgcta caggcatgcg cgaggccgtc
     2641 gacctcgagc agcgtcgcgg cgacgcggc
//
LOCUS       DASVLN010000609         5896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_463005, whole genome shotgun sequence.
ACCESSION   DASVLN010000609 DASVLN010000000
VERSION     DASVLN010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463005"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..864
                     /locus_tag="VF453_14580"
     CDS             58..864
                     /locus_tag="VF453_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7688937.1"
                     /translation="MSPGFALELHDVRKRFGRTEIIRGATLQVRRGERVAIIGPNGAG
                     KSTLFNLVSGRLAPSAGRILLEGRRIDGLPPHRVHRLGLARSFQVSNLFGRLSTFENL
                     RCAVLWSLGYRYTFWRFLSGLDDANARAAELLAMLRLEHRRDVPAAELAYAEQRALEI
                     GMTIAGGSSVVLLDEPTAGMSESETRRFLQLIRDVTEGRTLLAVEHDMGVVFGLADRI
                     AVLVHGEFIAFDTPEAVRADPRVQEAYLGVPRGAADGAAATSSPIRERGR"
     gene            864..1559
                     /locus_tag="VF453_14585"
     CDS             864..1559
                     /locus_tag="VF453_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7688938.1"
                     /translation="MLELEQLHAFYGKSHVLHGVTLRVGPGEIVALLGRNGSGRSTTA
                     KAVMGLVRTQGRIDWRGQSLRGLAPYQVARRGIGYVPESRDVFPRLTVHQNLELGRQP
                     GRRDARWGFEDMYALFPGLRERRDVEAGVLSGGEQQMLTLCRSLMGDPGLLLIDEPTE
                     GLAPRVVEQVAAFLLALKARGIAVLLVEQKLTIALEISERCVVMGHGRVVFEGTPAQL
                     RAHAGVRREWLEV"
     gene            2031..2555
                     /locus_tag="VF453_14590"
     CDS             2031..2555
                     /locus_tag="VF453_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688939.1"
                     /translation="MSAQLISNRISLDIPDDDMAAIVAAIQTLEDKLVPHLVDLNPED
                     RRSLPKMGAKTVDFVSKTLDLTSANELFRPAFVDLGEFERDLAAVGALRKLQQPLSKI
                     SDMVGDSLLLSGSEAYAAALACYQVIKSAARLNTPGAMVAAADLASRFPRRVARAKDP
                     QAPTAAPSQPGTGH"
     gene            2667..3026
                     /locus_tag="VF453_14595"
     CDS             2667..3026
                     /locus_tag="VF453_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688940.1"
                     /translation="MHLLATRARLAALLLVPLAGTAGAASISVRLAPEVDAKALVVDC
                     LKSSVGRCVVAVQAPGEAQPRVVVVPAQGSQRVEGVPTGSRYCVDVQEQPSWDSCPKK
                     ELGPAMTELDQVFFDGK"
     gene            3110..4006
                     /locus_tag="VF453_14600"
     CDS             3110..4006
                     /locus_tag="VF453_14600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7688941.1"
                     /translation="MPRKTAADFPREVLSLFDQYVHGAISRRGFLDGAARYAAAGGMT
                     AAGLLEALSPDFAAAQQVAPSDPRIVATTLAFDSPAGLGKASGLLVRPARSPARIPAI
                     LVVHENRGLNPHIEDIARRLALEGYLAFAPDALFTVGGYPGDEDKARELFGKLDPKKI
                     QADFVAAAAVLQAQPGTNGRVGCVGFCFGGGVVNHVATQWPELAAGVPFYGAAPRLED
                     VPRIKAPLLLHFAQHDDRINATWPPYEAALKAAHAKFEAHVYPNTEHGFNNDTTPRYD
                     AEAAALAWERTLTFFAHTLRTA"
     gene            4110..5024
                     /locus_tag="VF453_14605"
     CDS             4110..5024
                     /locus_tag="VF453_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7688942.1"
                     /translation="MSHPIARRALRAAAALAVSALPGIAPAQDFSKVEIRATELARDF
                     HTLQGQGGTVSVLSGPDGVLVVDAQFAPLTDKLVAAIRTFSDKPIRFLVDTHVHPDHT
                     GGNENFARLGATILARDQLRERLEKPAVNLDGSVRPAQPAAALPVVTYDAPVSLYLDG
                     EHVVLMPVRRAHTDGDTVVIFPGHDIMAVGDIFRSLGYPYVDRASGGTLAGTLEALSE
                     VADRAGPATRIVPGHGPIVGREALLAQRDMIIALRAKVLAQVQQGRTLEQVIASKPTA
                     EWDARVPQGPQTADRFVKWVYEEVAADR"
     gene            5119..5763
                     /locus_tag="VF453_14610"
     CDS             5119..5763
                     /locus_tag="VF453_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7688943.1"
                     /translation="MSKVKVACFGMSIDGFGAGPRQSLDDPLGVGGPSLMQWVFPTRT
                     FQRMHGDGAGGETGVDDDFAARGFAGVGAWILGRNMFGPVRGPWPDESWKGWWGDEPP
                     YHVPVFVLTHHARDPLEMQGGTTFHFVTDGIHAALDAARKAAGGRDIRIGGGPATVRQ
                     YLQAGLVDELHLAVSPTLLGQGEDLLAGIDLVALGFRCVEQVASGRAMHVVLAR"
BASE COUNT          789 a         2171 c         2140 g          796 t
ORIGIN      
        1 ctgggacgag gcccaggccg cggtcgcgcg cgcgcaggcg ggcgccggga cgcgcgcatg
       61 agcccaggct tcgcgctcga gctgcacgac gtgcgcaagc gcttcggcag gaccgagatc
      121 atccgcggcg cgacgctgca ggtgcgccgc ggcgagcgcg tggccatcat cgggcccaac
      181 ggcgccggca agtcgacgct gttcaacctc gtcagcggcc gcctcgcgcc gagcgcgggc
      241 cgcatcctgc tcgaaggccg gcgcatcgac ggcctgccgc cgcatcgcgt gcaccggctc
      301 ggcctcgcgc gcagcttcca ggtcagcaac ctgttcgggc gcctgtcgac gttcgagaac
      361 ctgcgctgcg cggtgctgtg gtcgctcggc taccgctaca cgttctggcg cttcctgtcc
      421 gggctggacg atgcgaatgc gcgcgccgcg gagctgctcg cgatgctgcg cctcgagcat
      481 cgccgcgacg tcccggccgc ggagctcgcc tacgccgagc agcgcgccct cgagatcggc
      541 atgaccatcg ccggcggctc gtccgtcgtg ctgctggacg agcccaccgc cggcatgagc
      601 gagagcgaga cgcgccgctt cctgcagctg atccgcgacg tcaccgaggg ccgcacgctg
      661 ctcgcggtgg agcacgatat gggggttgtc ttcggcctgg ccgaccgcat cgcggtgctg
      721 gtgcacggcg agttcatcgc cttcgacacc cccgaggcgg tgcgcgccga cccgcgcgtg
      781 caggaggcat acctcggcgt gccgcgcggc gccgccgatg gggcggcggc gacgtcgtcg
      841 ccgatacggg aacggggcag gtgatgctcg agctggagca gctgcacgcc ttctacggca
      901 agagccatgt gctgcacggc gtgacgctgc gcgtggggcc gggcgagatc gtcgcgctgc
      961 tgggccgcaa cggctcgggg cgctcgacca ccgcgaaggc cgtgatgggc ctggtgcgca
     1021 cgcagggccg gatcgactgg cgcgggcagt cgttgcgcgg cctggcgccc taccaggtcg
     1081 cgcgccgcgg catcggctac gtgcccgaga gccgcgacgt cttcccgcgc ctgaccgtgc
     1141 accagaacct cgagctcggc cgccagcccg gccggcgcga cgcgcgctgg ggcttcgagg
     1201 acatgtacgc gctgttcccc ggcctgcgcg agcgccgcga cgtcgaggcc ggcgtccttt
     1261 ccggcggcga gcagcagatg ctcacgctgt gccgctcgct gatgggcgac cccgggctgc
     1321 tgctgatcga cgagcccacc gagggcctcg cgcccagggt cgtcgagcag gtcgcggcct
     1381 tcctgctcgc gctcaaggcg cgcgggatcg ccgtgctgct ggtggagcag aagctcacga
     1441 tcgcgctgga gatctccgag cgctgcgtcg tgatgggtca cgggcgcgtc gtgttcgagg
     1501 gcacgccggc gcagctgcga gcgcacgccg gagtgcggcg ggagtggctg gaggtgtgag
     1561 gccggacgac gacctgcttg cgcctgcaag cggtgccgga tcgaggttcg atggctcgtg
     1621 cccgttcttc gacgcgtcgc gaaatggcgg gggcggcctg gcccggagcc tgggccgcct
     1681 gcctccgccg ggcgacgatg cactgatttt cagcagggcc gctgccggcg agctcgggtc
     1741 gcgggcccgg cggctcgggc gatgcgcgga cgaagcgcat cgcgggcgat cgacgcagcg
     1801 gcgtcgaagc gtcgaggcca cgcgtcgaag aagcgatcct gcgcgtcgag gatcggggct
     1861 gcgaccgcgc ttgcgggggc gcggccgtcg ccggcggccg gcgacccgtc cccctagttc
     1921 cgccggattc cctagttcgg agctcgcgat acggtgcccc gtatcagaag aatggcgcgc
     1981 cgccatggcg aggtgcttcg gcggcatacc cacagggaag gaagaagtcc gtgtccgctc
     2041 aattgatcag caatcgcatc tccctcgaca tccccgacga cgacatggcg gccatcgttg
     2101 ccgcgatcca gacgctggag gacaagctgg tgccccacct cgtcgacctg aatccggagg
     2161 atcgccggtc cttgccgaag atgggcgcga agaccgtgga cttcgtgtcc aagacgctgg
     2221 acctgacttc ggccaacgag ctgttccggc ccgccttcgt cgacctcggc gagttcgagc
     2281 gcgacctggc ggccgtcggg gcgctgcgca agctgcagca gccgctgagc aagatctcgg
     2341 acatggtcgg cgacagcctg ctgctgtccg gcagcgaggc ctatgccgcg gcgctggcct
     2401 gctaccaggt gatcaagtcg gccgcgcggc tgaacacgcc gggtgcgatg gtcgccgccg
     2461 ccgacctggc cagccgcttc ccgcgccgcg tcgcacgcgc gaaggatccg caggcgccga
     2521 cggccgcgcc gtcgcagccc ggtaccgggc actgaccgag ggggcgtcgg cgcggacgag
     2581 ccggggcccg ggccgaccct gaccgcgcgg cacgtggcga gggcccacaa tcggcctccc
     2641 ctgaaatccg aagcgtcgac aagcccatgc acctccttgc cacccgcgcc cgcctggccg
     2701 cgctgctcct cgtcccgctg gccggcacgg ccggggcggc ctcgatctcc gtgcgcctcg
     2761 cgccagaggt cgacgcgaag gcgctcgtgg tcgactgcct gaagtcctcg gtcggccgct
     2821 gcgtggtcgc cgtgcaggcc ccgggcgagg cgcagccgcg cgtcgtcgtg gtcccggccc
     2881 agggctcgca gcgcgtcgag ggcgtgccca cgggctcgcg ctactgcgtc gacgtgcagg
     2941 aacagccgtc gtgggacagc tgcccgaaga aggagctcgg cccggcgatg accgagctcg
     3001 accaggtctt cttcgacggc aagtgacgcg tcgcgagagt ccctggtctc gacgttggcc
     3061 cgagggcggt gtacacagcg ccatcccgac caacgacccc ggagcgccca tgccccgcaa
     3121 gaccgccgcc gacttcccgc gcgaagtgct cagcctgttc gaccagtatg tgcacggcgc
     3181 gatctcgcgg cgcggcttcc tggacggcgc cgcccggtac gctgccgccg gcggcatgac
     3241 cgccgcgggc ctgctcgagg cgctgagccc ggacttcgcg gccgcgcagc aggtcgcccc
     3301 ctcggacccg cgcatcgtcg ccacgacgct ggccttcgac tcccctgcgg gcctgggcaa
     3361 ggcgagcggc ctgctcgtgc ggcccgccag gtcgccggcc aggatcccgg cgatcctcgt
     3421 cgtgcacgag aaccgcggcc tgaacccgca catcgaggac atcgcgcgcc gcctcgcgct
     3481 cgagggctac ctcgcgttcg cgcccgacgc gctgttcacc gtcggcggct accctggcga
     3541 cgaggacaag gcgcgcgagc tgttcggcaa gctcgacccg aagaagatcc aggccgactt
     3601 cgtggccgcc gccgcggtgc tgcaggcgca accgggcacc aacggccgtg tcggctgcgt
     3661 cggcttctgc ttcggtggcg gcgtggtcaa ccacgtcgcg acgcagtggc ccgaactcgc
     3721 cgccggcgtg cccttctacg gcgcggcccc gaggctcgag gacgtgccgc gcatcaaggc
     3781 accgctgctg cttcacttcg cgcagcacga cgaccgcatc aacgccacgt ggccgccgta
     3841 cgaggccgcg ctcaaggccg cgcacgcgaa attcgaggcg cacgtctacc cgaacaccga
     3901 gcacggcttc aacaacgaca cgaccccgcg ctacgacgcc gaggccgcgg cgctcgcctg
     3961 ggagcgcacg ctgaccttct tcgcgcacac cttgcgaacg gcctgagctg tgcgcatcga
     4021 acgtcccggc gattggcggg gccggcggtt cgtccatact gcgcgctgcc gcaacaccca
     4081 cacggcgccg agccgccagg aggtccccga tgtcccaccc gatcgcccgc cgcgccctgc
     4141 gcgccgccgc ggccctggcc gtgtccgcgc tgcccggcat cgcgccggcg caggacttct
     4201 ccaaggtcga gatccgcgcc accgagctcg cgcgcgactt ccacacgctg caggggcagg
     4261 gcggcacggt cagcgtgctc tcggggccgg acggcgtgct ggtcgtcgac gcgcagttcg
     4321 cgccgctgac cgacaagctc gtcgctgcga tccgcacgtt cagcgacaag ccgatccgtt
     4381 tcctggtcga tacccacgtc catcccgacc acaccggcgg caacgagaac ttcgcccgcc
     4441 tcggcgcgac catcctcgcg cgcgaccagc tgcgcgagcg cctcgagaag cccgccgtca
     4501 acctcgacgg cagcgtccgc ccggcgcagc ccgccgcggc gctgccggtc gtgacctacg
     4561 acgcgccggt ctcgctgtac ctcgacggcg agcacgtggt gctgatgccc gtccggcgcg
     4621 cgcacaccga cggcgacacg gtggtgatct tccccggcca cgacatcatg gccgtgggcg
     4681 acatcttccg cagcctcggc tatccgtacg tcgaccgcgc gagcggcggc acgctcgccg
     4741 gcacgctgga ggcgctcagc gaggtcgccg accgcgccgg cccggccacc cgcatcgtcc
     4801 ccggccacgg cccgatcgtc ggccgcgagg cgctgcttgc ccagcgcgac atgatcatcg
     4861 cgctgcgcgc caaggtgctg gcgcaggtgc agcagggccg cacgctggag caggtgatcg
     4921 cgagcaagcc gaccgccgag tgggacgccc gggtgccgca gggaccgcag acggcggacc
     4981 ggttcgtcaa gtgggtgtac gaggaggtcg cggcggatcg gtgaagcccg cggcgctggg
     5041 gtatcgtccg cagcgggcga cgatgccggc ttcgcgcgcg tcgcgcccgc acgcactcac
     5101 ttgcaaggga gacgcctcat gtccaaggtc aaggtggcct gcttcggaat gtccatcgac
     5161 ggtttcggcg ccggtccgcg gcagagcctc gacgacccgc tgggcgtcgg cggcccgtcg
     5221 ctgatgcagt gggtcttccc gacccgtacg ttccagcgca tgcacggcga cggcgccggc
     5281 ggcgagacgg gcgtcgacga cgacttcgcg gcgcgcggct tcgccggcgt cggcgcctgg
     5341 atcctgggcc gcaacatgtt cggcccggtc cgcggcccct ggcccgacga gtcgtggaag
     5401 ggctggtggg gcgacgagcc gccgtaccac gtcccggtct tcgtgctcac gcatcacgcg
     5461 cgcgaccccc tcgagatgca gggcggaacg accttccact tcgtcaccga cggcatccac
     5521 gccgcgctgg acgcggccag gaaggccgcc ggcgggcgcg acatccgcat cggcggcggc
     5581 ccggcgaccg tccgccagta cctgcaggcg gggctggtgg acgagctgca cctggcggtg
     5641 tcgccgacgc tgctcggcca gggcgaggac ctgctcgcgg ggatcgacct ggtggcgctc
     5701 ggcttccggt gcgtggagca ggtcgcgtcg gggcgggcga tgcacgtggt gctggcgaga
     5761 tagtcccttg cggcggcgga cggctgcgcc gcgggcgccg ggccgggcac gtcgtcgggc
     5821 acccgggggg cctggcagtc accggatcac gccgaggcga cccggccccg ggtgggcagc
     5881 gcgagcgtga agctgg
//
LOCUS       DASVLN010000610         4187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_407694, whole genome shotgun sequence.
ACCESSION   DASVLN010000610 DASVLN010000000
VERSION     DASVLN010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407694"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1726
                     /gene="phaC"
                     /locus_tag="VF453_14615"
     CDS             62..1726
                     /gene="phaC"
                     /locus_tag="VF453_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX7688944.1"
                     /translation="MAGFLRAVSGLALPSSALSSLQEDYVAQATKLWNDAVDQIPVAA
                     TLAGKPAPADKPAPRPIGDRRFAAADWLGNPAAAFTAQLYLLNARTLTKMADSLQGDD
                     KTRARVRFAVQQWVDATSPANYLALNPEAQRKAIESKGESLSAGLRHLWGDVQQGHLS
                     QTDESVFEVGRNVATTAGSVVFENEYFQLIEYAPLTPKVHARPMLFVPPCINKYYILD
                     LQPENSLIRHTVSQGHRTFVVSWRNPDDSMADRTWDDYIEHAVIRAVDVVQEITGAKT
                     INTLGFCVGGTLLATGLAVLAARGRQPAESLTLLTTFLDFSDTGVLDIFVDEASVQLR
                     EMTLGEHAPGGPRLLRGGELASTFSFLRPNDLVWNYVVGNYLKGEKPPAFDLLYWNSD
                     ATNMPGPMYCWYLRNTYLDNALREPGRLTVCGVPLDLSKIAAPAYLYGSREDHIVPWK
                     GAYQSTRILGGDKRFVLGASGHIAGVINPPKANKRSHWINEASLPESPDDWLAGATEH
                     PGSWWTDWSRWLAGFGGGEVNAPRAPGGRGHAAIEPAPGRYVKQKA"
     gene            1835..3019
                     /locus_tag="VF453_14620"
     CDS             1835..3019
                     /locus_tag="VF453_14620"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7688945.1"
                     /translation="MSEDIVIVAAARTAVGRFGGSLAKVPASELGATVLRALLERSRL
                     QGDQVGEVILGQVLQAGTGQNPARQAVIKAGMPNAVPAMTINKVCGSGLKAVMLAAQA
                     IRDGDSEIVIAGGQESMSMAPHVLPNSREGQRMGDWKLVDTMINDGLWDVYNQYHMGI
                     TAENVAKKYGITREQQDALAFASQSKAAAAQDAGHFKEEIVGVAIPQKKGDPIVFDAD
                     EFINRKSTPEALAALRPAFDKAGSVTAGNASGLNDGAAGVVVMSAKKAAALGLKPLAR
                     IASYASAGLDPAFMGMGPVPAARKALERAGWKPADLDLLEINEAFAAQACAVHNEMGW
                     DMSKVNVNGGAIAIGHPIGASGARILVTLLHEMKRRGARRGIASLCIGGGMGVALTVE
                     RE"
     gene            3147..3884
                     /gene="phbB"
                     /locus_tag="VF453_14625"
     CDS             3147..3884
                     /gene="phbB"
                     /locus_tag="VF453_14625"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX7688946.1"
                     /translation="MSQKLAYVTGGMGGIGTAICQRLHRDGFKVIAGCGPSRDHAKWI
                     AEQKALGYTFHASVGNVAEWDSTVAAFTQAKEEHGAIDVLVNNAGITRDRMFLKMTRE
                     DWDAVIGTNLTSMFNVTKQVVADMVERGWGRIIQISSVNGAKGQAGQTNYSAAKAGMH
                     GFSMALALEMASKGVTVNTVSPGYIGTDMVRAIKPEVLEKIVATIPVKRLGTPEEIAS
                     IVGWLAGDESGFTTGAEFAVNGGLHMG"
BASE COUNT          685 a         1509 c         1458 g          535 t
ORIGIN      
        1 ggcggcccgg gccgccggcg ccgagccgcc cggcgccacg ccggaggcct tcggccacac
       61 gatcgccggc ttcctgcggg ccgtgagcgg cctcgcgctg ccgtcgtcgg cgctctcgtc
      121 gctgcaggag gactacgtcg cccaggccac gaagctctgg aacgacgccg tcgaccagat
      181 cccggtcgcc gccaccctgg ccggcaagcc cgcgccggcc gacaagcccg cgccccggcc
      241 catcggcgac cgccgcttcg ccgccgccga ctggctgggc aaccccgccg ccgccttcac
      301 ggcgcagctg tacctgctca acgcgcgcac gctgacgaag atggccgaca gcctgcaggg
      361 cgacgacaag acgcgcgccc gcgtgcgctt cgcggtgcag cagtgggtcg acgccaccag
      421 ccccgccaac tacctcgcgc tcaaccccga ggcccagcgc aaggcgatcg aatcgaaggg
      481 cgagagcctg tcggccggcc tgcgtcacct gtggggcgac gtgcagcagg ggcacctgtc
      541 gcagaccgac gagagcgtgt tcgaggtcgg ccgcaacgtc gcgacgaccg cgggctcggt
      601 ggtgttcgag aacgagtact tccagctgat cgagtacgcg ccgctcacgc cgaaggtgca
      661 tgcgcggccg atgctgttcg tgccgccgtg catcaacaag tactacatcc tcgacctgca
      721 gccggagaac tcgctgatcc gccacacggt ctcgcagggc caccgcacct tcgtcgtgag
      781 ctggcgcaat cccgacgact cgatggcgga ccgcacctgg gacgactaca tcgagcacgc
      841 cgtgatccgc gccgtcgacg tggtgcagga gatcaccggc gcgaagacga tcaacacgct
      901 gggcttctgc gtcggcggca cgctgctggc caccggcctc gccgtgctcg cggcgcgcgg
      961 ccgccagccg gccgagagcc tgacgctgct cacgaccttc ctcgacttct ccgataccgg
     1021 cgtcctcgac atcttcgtcg acgaggcgtc ggtgcagctg cgcgagatga cgctcggcga
     1081 gcacgccccc ggcgggccac gcctgctgcg cggcggcgag ctcgcgagca ccttcagctt
     1141 cctgcgcccg aacgacctcg tctggaacta cgtcgtcggc aactacctga agggcgagaa
     1201 gccgcccgcg ttcgacctgc tgtactggaa cagcgacgcc accaacatgc cggggccgat
     1261 gtactgctgg tacctgcgca acacctacct ggacaacgcg ctgcgcgagc ccggccggct
     1321 gacggtctgc ggcgtgccgc tggacctgtc gaagatcgcg gctcccgcct acctctacgg
     1381 ctcgcgcgag gaccacatcg tgccgtggaa gggcgcgtac cagtccaccc gcatcctggg
     1441 cggcgacaag cgcttcgtgc tgggcgcctc gggccacatc gcgggcgtca tcaacccgcc
     1501 gaaggccaac aagcgcagcc actggatcaa cgaggcgtcg ctgcccgaat cgcccgacga
     1561 ctggctcgcc ggcgcgaccg agcatccggg cagctggtgg accgactggt cgcgctggct
     1621 cgccggcttc ggcggcggcg aggtcaacgc gccgcgcgcg cccggcggcc gcggccacgc
     1681 ggcgatcgag ccggcgccgg gccgctacgt caagcagaag gcttgactcg gctcaagccg
     1741 tggcggccgg acgacagcta gcctcgacga ctcgccgcga cagcgatcag tcataccccg
     1801 tcccgtatca tcgcccccct gactggagac catcatgagt gaagacatcg tcatcgtcgc
     1861 cgctgcccgc accgccgtcg gccgcttcgg cggcagcctg gccaaggtgc cggcctcgga
     1921 gctgggcgcc accgtcctgc gcgcgctgct ggagcgctcg cgcctgcagg gcgaccaggt
     1981 gggcgaggtg atcctgggac aggtgctgca ggccggcacc ggacagaacc cggcccgcca
     2041 ggcggtcatc aaggccggca tgcccaacgc ggtgccggcg atgaccatca acaaggtctg
     2101 cggctcgggc ctcaaggccg tgatgctcgc ggcgcaggcg atccgcgacg gcgattccga
     2161 gatcgtgatc gccggcggcc aggagagcat gagcatggcg ccgcacgtgc tgccgaactc
     2221 gcgcgagggc cagcgcatgg gcgactggaa gctggtcgac accatgatca acgacggcct
     2281 gtgggacgtg tacaaccagt accacatggg catcaccgcg gagaacgtcg ccaaaaagta
     2341 cggcatcacc cgcgagcagc aggacgcgct cgccttcgcc tcgcagtcca aggcggccgc
     2401 ggcacaggac gccggccact tcaaggagga gatcgtcggc gtcgccatcc cgcagaagaa
     2461 gggcgacccg atagtcttcg acgccgacga gttcatcaac cgcaagtcga cgccggaggc
     2521 cctggccgcc ctgcgtcccg ccttcgacaa ggccggctcc gtcaccgccg gcaacgcctc
     2581 gggcctgaac gacggcgccg cgggcgtggt cgtgatgtcg gcgaagaagg ccgcggcgct
     2641 gggcctgaag ccgctcgcgc gcatcgcctc gtacgcgagc gccggcctcg acccggcctt
     2701 catgggcatg ggcccggtgc cggcggcgcg caaggcgctg gagcgcgcgg gctggaagcc
     2761 ggccgatctc gacctgctcg agatcaacga ggccttcgcg gcccaggcct gcgcggtcca
     2821 caacgagatg ggctgggaca tgagcaaggt caacgtcaac ggcggcgcga tcgcgatcgg
     2881 gcacccgatc ggtgcgtccg gtgcgcgcat cctcgtcacg ctgctgcacg agatgaagcg
     2941 ccgcggcgcc aggcgcggca tcgcgtcgct gtgcatcggc ggcggcatgg gcgtggcgct
     3001 gaccgtcgag cgcgagtgaa gcggaacggc cgaggccgcc tcacgcaggg tagcccctga
     3061 gacgtgcggc cgcgatgccg cgcgatagtg ggtccggtcc cacgacacga acgaaccccc
     3121 aaacttctcg aaaaggagac aacgagatga gccagaaact agcgtacgtc accgggggca
     3181 tgggcggcat cggcaccgcg atctgccagc gcctgcaccg cgacggcttc aaggtcatcg
     3241 ccggctgcgg cccgagccgc gaccacgcca agtggatcgc ggagcagaag gccctcggct
     3301 acaccttcca cgcctcggtc ggcaacgtcg ccgagtggga ttccaccgtc gccgccttca
     3361 cgcaggccaa ggaggagcac ggggcgatcg acgtgctggt caacaacgcc ggcatcacgc
     3421 gcgaccgcat gttcctgaag atgacgcgcg aggactggga cgcggtcatc ggcaccaacc
     3481 tgacctcgat gttcaacgtg acgaagcagg tggtcgcgga catggtcgag cgcggctggg
     3541 gccgcatcat ccagatctcg tcggtgaacg gcgccaaggg ccaggccggg cagaccaact
     3601 actcggccgc caaggcgggc atgcacggct tctcgatggc gctggcgctg gagatggcca
     3661 gcaagggcgt gacggtcaac accgtgagcc cgggctacat cggcaccgac atggtccgcg
     3721 cgatcaagcc cgaggtgctc gagaagatcg tcgccaccat ccccgtcaag cgcctgggca
     3781 cgcccgagga gatcgcctcc atcgtgggct ggctggccgg cgatgagtcc ggcttcacga
     3841 ccggcgccga gttcgcggtg aacggcggcc tgcacatggg ctgacgcgct tcgcgccacc
     3901 gcggcggacc gcgcgtccgc cgcaggaacg accccgcggc ctgggccccg gggtcgtttt
     3961 cgtttgggcg ggtactgcgc gctggccggc gcgcgcgcga catgcgagga tgcggcccca
     4021 cgacaacgac acggggggaa ccatggaata tcggctgcgc gcggacgcgg gagccgcggc
     4081 atgaagcggc gcgcgtcagc ggccgtcgcg atcgtcgtcg gcatcgcggt cgccgcgggc
     4141 ctggctcgcg gctggccgga gggcccgtcg gcgcgccggg tgcggca
//
LOCUS       DASVLN010000611         7401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_141567, whole genome shotgun sequence.
ACCESSION   DASVLN010000611 DASVLN010000000
VERSION     DASVLN010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141567"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VF453_14630"
     CDS             <1..874
                     /locus_tag="VF453_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7688947.1"
                     /translation="APRAEPLPEASPAPAPRRSQAGAALLEVQELRVRFPIRKGLFKR
                     GRAYFDAVDGVSFTVGERETLALVGESGCGKTTTGKAIVQLLRTSAAFEGRALLQGRN
                     LFELSGAALTQARRDIQIIFQDPFASLDPRMRVAEILEEGMAALLPEVDAAERRRRIE
                     VLVDRCGLRRDALGRYPHEFSGGQRQRIAIARALAVQPRLIVCDEPTSALDVSVQAQI
                     LNLLHELQRELGVSYLFITHNIGVVEYIADRVAVMRAGRIVEQGDCAAVLESPRDPYT
                     RNLIDAVPRLKLPA"
     gene            1330..4380
                     /locus_tag="VF453_14635"
     CDS             1330..4380
                     /locus_tag="VF453_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7688948.1"
                     /translation="MFQRTKICSGLLIAIGSGAFSVSGVALAQDTSVQRVEIIGSSIK
                     RVDTETDAPITTISHEEIAKTGATTVEELMRHVTANNSSNSAVAASASGATTGGISTI
                     SLRGLGPERTLILVNGQRSAPYGDPVDSVSVDVDSIPVAAIERIEVLKEGAGAIYGSD
                     AVAGVVNFVLRKDYHGVEATGYYGQSYDGKGSVSKGSIVAGFGGDKGNATFVVTAEHD
                     MPLYGRDRSFANTSIHPEHDNYSASSGSVPANTSIPQLGLTNPLVPVIWDPTIQKKVT
                     NQDGSVVTATTGGYKATGAGNCGPLSTYSSTFGPNDCLFDPAGYTSLIPDVHRVGVLF
                     TGHYDLTDDVRLHLDASVTNKKQFVSIQPAPIGTYVGIPWALTTANPYYPTTFVSALT
                     HAAAGATIVGGDNNGKLQYDPSKHIDATDPTGYTPTLDLRYRPFITGSRNLTDEASNL
                     RVSGGIDGTVAGWDYTANLLFSGSAVNESLQSGYFRINGGAASGGPGIEALLNGQDKD
                     SAGNTLWVNPFGDNSAAVLAAAQATNFTGNAFQTRTSLQDAQLKFSRDDVMKLPGGNM
                     GAAFGVELRRDEFKLTAAPALASGDISGYGGNFPDFDRSRNVASFFGELDVPVIKGLD
                     VDGAVRYDHYGSTTNPNSLAGGTDTLNSIAISDNNNNTDYLPGDVVARVAQEGTQNAG
                     GFGQTTGKLGAKWTISKQLLFRGTISTGFRAPSLLDLYNPLQAGVSAFVNDPKRCSAN
                     PLDCGTQYNQYSGGRGDLKPERSLTYTLGTVMEPVKDINFSLDYFHTKLRDQIAVLGA
                     DYLLNHEAQYQSSIIRGPSDGVGQAGPIIAIDQRTQNITEAIVAGLDFDANATFRTAA
                     GTFGAGLGGTWMTKWDSVNPDGSRENEIGTTSSTVTGMIPRMKLSLEGSYAMPNNFFS
                     GSVIYNWQSHGTDVCGTLDQDDLGNCADGISPPKVKAYGTVDLQGVINPSKMMSATVG
                     LKNAFNTKPPYVNGAGGAFQSGYDPTYVDPHGRFWYVSATLRFQ"
     gene            complement(4617..4904)
                     /locus_tag="VF453_14640"
     CDS             complement(4617..4904)
                     /locus_tag="VF453_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688949.1"
                     /translation="MRTAFALLGLAIVAGCASEPAPPQQVASAATPAAKPNQTCHKEA
                     PMGSYRPQTVCYDADGDQIDPNDPNKQRAIDDAARQMGAMQRQMGRAGVGR"
     gene            complement(5046..5804)
                     /locus_tag="VF453_14645"
     CDS             complement(5046..5804)
                     /locus_tag="VF453_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX7688950.1"
                     /translation="MDISGKVFIVTGAASGLGEGTARMLAGNGGKVVLADLQEERGQA
                     VAKELGQVFVRCDVTQEADGRRVVDEARKLGKLMGLVNCAGIAPAARVVGKTGAHALD
                     LFAKVVTVNLVGSFNMIRLAAEAMSGNEPEPTGERGVLISTASVAAFDGQIGQAAYAA
                     SKGGVVGMTLPIARDLAKSGIRNMTIAPGIFGTPMLFGMPQEVQDALAASVPFPSRLG
                     TPADYAKLVHQIVTNEMLNGEVIRLDGAIRMAPR"
     gene            complement(5972..6628)
                     /gene="adk"
                     /locus_tag="VF453_14650"
     CDS             complement(5972..6628)
                     /gene="adk"
                     /locus_tag="VF453_14650"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX7688951.1"
                     /translation="MRLILLGAPGAGKGTQAAFICQKYGIPQISTGDMLRAAVKAGTP
                     LGLAAKQVMDAGGLVSDDLIISLVKERIAQPDCVNGFLFDGFPRTIPQADAMKAAGVK
                     LDLVLEIDVPDAAIVERMDGRRVHPASGRTYHVVNNPPKVDGVDDVTGEPLVQRPDDS
                     AEIVRKRLATYHEQTEPLVAYYAQWAASGDPQAPRCRKISGTGTVEEITQRALQALEN
                     "
     gene            complement(6793..>7401)
                     /gene="kdsB"
                     /locus_tag="VF453_14655"
     CDS             complement(6793..>7401)
                     /gene="kdsB"
                     /locus_tag="VF453_14655"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX7688952.1"
                     /translation="ARVADACAANGVEAVLTRGDHASGSDRLAEACELLGLAGAEVVV
                     NVQGDEPLIDPALVRQCAGLLETDAEAVVATAAHPIHDAADFANPNVVKVVLDARQRA
                     LYFSRAPLPWWRDASAPGQPALPEGALRHIGIYAYRAGYLRQFPSLAMSPLEKLESLE
                     QLRVLWHGDRIVVHIAADAPPPGVDTPEDLERVRALVAGRGR"
BASE COUNT         1146 a         2655 c         2469 g         1131 t
ORIGIN      
        1 ggcgccccgt gccgagccgc tgcccgaggc gtcgcccgcg ccggcgccac ggcgctcgca
       61 agccggcgcc gcgctgctgg aggtgcagga gctgcgcgtg cgattcccga tccgcaaggg
      121 acttttcaag cgcggccgcg cgtacttcga cgcggtcgac ggcgtctcgt tcacggtcgg
      181 cgagcgcgag acgctcgcgc tggtgggcga gtcgggttgc ggcaagacga cgacgggcaa
      241 ggcgatcgtg cagctgctgc gcaccagcgc cgcgttcgag ggccgcgcgc tgctgcaggg
      301 gcgcaacctg ttcgagctct ccggcgcggc gctcacgcag gcgcggcgcg acatccagat
      361 catcttccag gacccgttcg cctcgctcga cccgcgcatg cgcgtggccg agatcctcga
      421 ggaagggatg gcggcgctgc tgccggaggt cgacgcggcc gagcgtcgcc gccgcatcga
      481 ggtgctggtc gaccgctgcg gcctgcgccg cgacgcactg ggccgttatc cgcacgagtt
      541 ctcgggcggc cagcgccagc gcatcgcgat cgcgcgcgcg ctggcggtgc agccccggct
      601 catcgtctgc gacgagccga cctccgcgct ggacgtgtcg gtgcaggcgc agatcctgaa
      661 cctcctgcac gagctgcagc gcgagctggg cgtctcgtac ctgttcatca cgcacaacat
      721 cggtgtcgtg gaatacatcg ccgaccgcgt cgccgtgatg cgcgccggcc gcatcgtgga
      781 gcagggcgat tgcgccgcgg tgctggagag cccgcgcgac ccgtacacgc gcaatctcat
      841 cgatgccgtg ccgcgcctga agctgcccgc ctgagggccg cgcggcaggt gctccggcgg
      901 cggcccgcga gcggcctgcg ccgcggcccc cacgccactc cctgcggttg tttggcattg
      961 tctaggggac tgcagccggc gccgccgggt gcgtgatgga aaacccgcgc ctcccggggt
     1021 cccgtcgcgg ccgtccgcga acgcatcgcc gggggtattc ccgctctccg tgaagggatg
     1081 tcggcgagcg tgcgcggcga gttgcccgac tgtgacgaaa ccgactcgaa atccgaggcg
     1141 cccgcgcaac agactaccta gggaaaacgt ccaaaacggg tttccatcag tgagtccgcg
     1201 cagacgttca tggacaatat tttgtacaag ctttcgaagt cagttcatca gccgttcaca
     1261 aaccggcccg cgacttgctt gaaggctggg tctatcgatc aacctttcca ccccctcagg
     1321 agggaaccca tgttccaacg aacgaagatc tgttccggtc ttctgattgc catcggcagc
     1381 ggcgcgttca gcgtgtccgg cgtcgccctg gcccaagaca ccagcgtcca gcgcgtcgaa
     1441 atcatcggct cctcgatcaa gcgtgtcgac acggaaacgg acgccccgat cacgacgatc
     1501 tcgcacgagg agatcgcgaa gaccggcgcg acgaccgtcg aagaactgat gcgccacgtc
     1561 acggcgaaca actcgagcaa ctcggccgtc gcggcctccg cctcgggcgc caccaccggc
     1621 ggcatctcca ccatctcgct gcgcggcctc ggccccgagc gcaccctgat cctcgtcaac
     1681 ggccagcgca gcgcgccgta cggtgacccg gtcgactccg tctccgtcga cgtcgactcc
     1741 atccccgtcg ccgccatcga gcgcatcgaa gtcctgaagg aaggcgcggg cgccatctac
     1801 ggctcggacg ccgtcgcggg cgtcgtgaac ttcgtgctgc gcaaggacta ccacggcgtc
     1861 gaggcgaccg gctactacgg ccagtcctac gacggcaagg gttccgtctc caagggctcg
     1921 atcgtcgccg gcttcggcgg cgacaagggc aacgcgacct tcgtggtcac ggccgagcac
     1981 gacatgccgc tgtacggccg cgaccgctcg ttcgccaaca cctcgatcca ccccgagcac
     2041 gacaactact cggcgtcgtc gggttcggtt ccggccaaca cctccatccc gcaactgggc
     2101 ctgaccaacc cgctggtccc cgtcatctgg gatccgacca tccagaagaa ggtcacgaac
     2161 caggacggga gcgtcgtcac cgcgaccacc ggcggctaca aggccacggg cgccggcaac
     2221 tgcggcccgc tcagcaccta cagcagcacc ttcggtccga atgattgcct gttcgacccg
     2281 gccggctaca ccagcctgat ccccgatgtg catcgtgtcg gcgtgctgtt caccggtcac
     2341 tacgacctga cggacgacgt gcgcctgcac ctggacgcgt cggtgacgaa caagaagcag
     2401 ttcgtctcga tccagccggc tccgatcggc acgtacgtgg gcattccctg ggcgctgacg
     2461 acggccaacc cgtattaccc gacgaccttc gtcagcgcac tgacgcacgc cgctgcgggc
     2521 gcgacgatcg tgggcggcga caacaatggc aagctgcagt acgacccgtc caagcacatc
     2581 gacgcgacgg atccgacggg ctacaccccg acgctggacc tgcgctaccg cccgttcatc
     2641 accggcagcc gcaacctgac cgacgaagca agcaacctgc gcgtctccgg cggcatcgac
     2701 ggcaccgtcg cgggctggga ctacacggcc aacctgctgt tctccggcag tgcggtcaac
     2761 gagtcgctgc agtcgggcta cttccgcatc aacggcggcg cggccagcgg cggccccggc
     2821 atcgaggcgc tcctgaacgg ccaggacaag gattcggccg gcaacaccct gtgggtcaat
     2881 ccgttcggcg acaacagcgc cgcggtgctg gcagccgccc aggcgaccaa cttcaccggc
     2941 aacgccttcc agacgcgtac ctccctgcag gacgcccagc tgaagttctc gcgtgacgac
     3001 gtcatgaagc tgcccggcgg caacatgggc gccgccttcg gcgtggaact gcgtcgcgac
     3061 gagttcaagc tgaccgccgc gccggccctg gcctcgggcg acatctcggg ctacggcggc
     3121 aacttccccg acttcgaccg ttcgcgcaac gtcgccagct tcttcggcga actcgacgtg
     3181 ccggtgatca agggcctgga cgtcgatggc gccgtccgct acgaccacta cggctcgacg
     3241 accaacccga actcgctggc tggaggtacg gacacgctca attccatcgc catctcggac
     3301 aacaacaaca acaccgacta tctgcccggg gatgtcgttg cgcgagtggc ccaggaaggc
     3361 acccagaacg ccggcggctt cggtcagacg accggcaagc tgggcgccaa gtggacgatc
     3421 agcaagcagc tgctgttccg cggcacgatc agcacgggct tccgcgcgcc gtcgcttctg
     3481 gacctgtaca acccgctgca ggccggcgtg agcgcgttcg tgaacgaccc gaagcgttgc
     3541 tcggcgaacc cgctcgattg cggcacgcag tacaaccagt actccggcgg ccgcggcgac
     3601 ctgaagcccg agcgttcgct gacctacacg ctgggcaccg tgatggagcc ggtgaaggac
     3661 atcaacttct cgctggacta cttccacacg aagctgcgtg accagatcgc ggtcctgggc
     3721 gccgactacc tgctcaacca cgaagcgcag taccagtcga gcatcatccg cggcccgtcg
     3781 gacggcgtgg gtcaggccgg cccgatcatc gcgatcgacc agcgcaccca gaacatcacc
     3841 gaagccatcg tcgcgggcct ggacttcgac gccaacgcca ccttccgcac tgcggcgggc
     3901 acgttcggcg ccggcctcgg cggtacctgg atgaccaagt gggactcggt caacccggac
     3961 ggctcgcgcg agaacgagat cggcaccacg tccagcaccg tgacgggcat gatcccgcgc
     4021 atgaagctgt cgctggaagg ctcctacgcc atgccgaaca acttcttctc gggttcggtg
     4081 atctacaact ggcaatcgca cggcacggac gtctgcggca cgctggacca ggacgacctg
     4141 ggcaactgcg ccgacggcat ctcgccgccg aaggtcaagg cctacggcac cgtggacctg
     4201 cagggcgtga tcaacccgtc gaagatgatg tcggcgaccg tcggcctgaa gaacgccttc
     4261 aacaccaagc cgccgtacgt caacggcgcc ggtggtgcgt tccagtcggg ctacgacccg
     4321 acctacgtcg acccgcacgg ccgcttctgg tacgtcagcg ccacgctgcg tttccagtaa
     4381 ccagcgatca cgaccgacgc gcctcgcctg gcgcgtctcc ctcgaaagcc ggcttcgtgc
     4441 cggctttttt cgttgtcgcg cccggcggag gcggtgcgtg ccgtctcggt acgcgcatgg
     4501 gctcgcgcgt gagcgcatac acgcgcaccc gagggccgag cggacgtgaa aaggcccggg
     4561 atcaccgtcc tcgcggacgg cgggcccggg cccgtgtcag tggctgtcgt cgatcgtcag
     4621 cggccgacgc ccgcacgccc catctgccgt tgcatcgcgc ccatctggcg cgcggcgtcg
     4681 tcgatcgcgc gctgcttgtt cggatcgttc ggatcgatct ggtcgccgtc cgcgtcgtag
     4741 cacacggtct gcggccgata cgagcccatc ggcgcttcct tgtgacacgt ctggttcggc
     4801 ttcgcggccg gcgtggcggc ggaagccacc tgctggggtg gcgcgggttc gctggcgcag
     4861 ccggcgacga tcgccagccc gagcagggcg aatgcagtgc gcatggggac tctcccggat
     4921 gttgtggttg accgtcgagt ctagcaacgc gcgacacgac tgcccacatg ggagacccgc
     4981 atcgcgcgcc gccgcgcggc ccatccggcg ccgtgccgac gacgcgcgcg cgcgtcgcgc
     5041 ggctctcagc gcggcgccat gcggatcgcg ccgtccaggc ggatcacctc gccattgagc
     5101 atctcgttgg tgacgatctg gtgcaccagc ttcgcgtagt cggcgggcgt gcccaggcgg
     5161 ctggggaagg gcacgctggc ggcgagcgcg tcctgcacct cctgcggcat gccgaacagc
     5221 atcggcgtgc cgaagatccc cggcgcgatg gtcatgttgc ggatgccgct cttggcgagg
     5281 tcgcgcgcga tcggcagcgt catgccgacc acgccgccct tgctcgcggc gtaggcggcc
     5341 tggccgatct ggccgtcgaa ggccgcgacg ctggcggtgc tgatcagcac gccgcgctcg
     5401 ccggtcggct cgggctcgtt gccgctcatc gcctcggccg cgaggcggat catgttgaag
     5461 ctgcccacga ggttgaccgt caccaccttg gcgaacaggt ccagcgcgtg cgcgccggtc
     5521 ttgccgacga cccgggccgc cggcgcgatg cccgcgcagt tgacgaggcc catcagcttg
     5581 cccagcttgc gcgcctcgtc gaccacccgg cggccgtcgg cttcctgcgt cacgtcgcag
     5641 cgcacgaaaa cctggccgag ttccttggcg acggcctggc cgcgctcttc ctgcaggtcg
     5701 gccagcacga ccttgccgcc gttgccggcg agcatgcgcg ccgtgccttc gccgaggccc
     5761 gaggccgcgc cggtgacgat gaaaaccttg cccgagatgt ccattgattg gctcctggcc
     5821 gcggcgggac gcgcgcggcg actgacgttt acgtaaacgt caattgtggc ggcaatcgcg
     5881 ggcgctgcca agacgacgac aggcccgcgg cgcgaaccgc gggcctgtcc ggatggggtg
     5941 ggccgcgcgt cgcgcgcgcc ggcccggtgg ctcagttctc gagggcctgc agcgcgcgct
     6001 gcgtgatctc ctcgacggtg ccggtgccgc tgatcttccg gcagcggggc gcctgcggat
     6061 cgccggaggc ggcccattgc gcgtagtagg cgaccagcgg ctcggtctgc tcgtggtagg
     6121 tcgccaggcg cttgcgcacg atctcggcgc tgtcgtccgg gcgctgcacc agcggctcgc
     6181 cggtgacgtc gtccacgccg tccaccttgg gcgggttgtt gacgacgtgg tacgtgcggc
     6241 ccgaggccgg gtgcacgcgg cggccgtcca tgcgctcgac gatcgcggcg tcggggacgt
     6301 cgatctccag caccaggtcg agcttcacgc cggccgcctt catcgcgtcg gcctgcggga
     6361 tggtgcgcgg gaagccgtcg aacaggaagc cgttgacgca gtcgggctgg gcgatgcgtt
     6421 ccttcaccag cgagatgatg aggtcgtcgc tcaccagccc gccggcgtcc atcacctgct
     6481 tggcggcgag gcccagcggc gtgcccgcct tgaccgccgc ccgcagcatg tcgccggtgg
     6541 agatctgcgg gatcccgtat ttctggcaga tgaacgcggc ttgcgtgccc ttgcctgcgc
     6601 cgggagcacc cagaagaatc aatctcattg cttgtcgcct cggaagtcga taattatttt
     6661 tgctggcatg gccattgcaa ccgggagtca gaatatcacg cgcatcccga cgccccgctg
     6721 acgcgggacg ccctcgcgca ggcccggcgc cgccgagtcg tgccgttttt cacccggacc
     6781 gccgcgcggg ggtcaacggc cgcgcccggc gaccagcgcg cgcacgcgct ccaggtcctc
     6841 gggcgtgtcg acgcccggcg gcggtgcatc ggcagcgatg tgcaccacga tgcggtcgcc
     6901 gtgccacaac acgcgcagct gctcgagcga ctccagcttc tccagcggcg acatggccag
     6961 cgaggggaat tgtcgcagat accccgcccg gtatgcatag atcccgatgt ggcgcagcgc
     7021 accctcgggc agtgcgggct gtcccggcgc ggaagcgtcg cgccaccacg gcagcggcgc
     7081 gcggctgaag tagagcgcac gctggcgcgc gtcgagcacc accttcacga cgttcgggtt
     7141 cgcgaagtcc gcggcgtcgt ggatcgggtg ggccgccgtc gcgacgaccg cttcggcgtc
     7201 ggtctccagc aggccggcgc actggcgcac cagggcggga tcgatcagcg gctcgtcgcc
     7261 ctgcacgttg acgacgacct ccgcgccggc gagtcccagc agctcgcacg cctcggcgag
     7321 ccggtcgctg ccgctcgcgt ggtcgccgcg cgtgagcacg gcctcgaccc cgttggccgc
     7381 gcaggcgtcg gcgacgcgcg c
//
LOCUS       DASVLN010000612        15366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_246503, whole genome shotgun sequence.
ACCESSION   DASVLN010000612 DASVLN010000000
VERSION     DASVLN010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15366
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246503"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2250
                     /locus_tag="VF453_14660"
     CDS             <1..2250
                     /locus_tag="VF453_14660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7688953.1"
                     /translation="DAPRAKAQTLDLTEVVVTGTSTATSKMKSSVSVSTLDGDRIIDN
                     QPQNAADVLTAIPGLFVQSSGGGGNANVSVRGLPISAGGSRYLQFQEDGLPVLLFGDI
                     AFGNPDDFIRVDQTIDRVEAVRGGSASTLTTNGPGGIVNYITKTGDVPGGVLAVTSGL
                     GFQDERIDFAYGGTLAPKTNYFVGGFYESGVGPRRTAGGNAVEGGQIKGNVTRKVDAG
                     YVRLNFKLLDDRQPMFMPGPVNVVDGHIDTIPGIDPRKYTGYSPAIPVDTVLNDDNTS
                     TVVHMNDGMRTRSAAIGLETHLDLTQGWVLDDKFRRAANSGRWAAWYPGSAAATAAAG
                     TSYATGTKAGQAYSGLSMTSVAFDVDVKDLGNTTNDLKLTKTFEHLAGGRLAVGAGLF
                     LNDQAVNLVWNFNGYLTSVSEAPVLLNNATLGATQGGSLGPGFGGCCSRDYDGQYHTV
                     APYAFATFDLGGLTVDGSVREDNQHASGYYNIAQPVSATPGAALSYSTATVQPIDYAL
                     KRTEYSLGANYALNRQLAVFARYSDGASFNADRIMDLGPLDGSASIPVNTVKQLEGGV
                     KANLGHVSAFVTLFDAKVSESNYSATTQAAAANTYDATGVELESSYTSGGGWYVNVGA
                     TYTDSKTKSSTNAALVGLPANRQPKLIYQVGPGFETEHVDLGVQLVGIGSSKDTDTAT
                     SATVPYVKLPAYTLVNGHATYNFDDHTSFTLGFYNLFNRIGWTEVDSAGAARSVNGRT
                     LRGTLRYAF"
     gene            complement(2312..3304)
                     /locus_tag="VF453_14665"
     CDS             complement(2312..3304)
                     /locus_tag="VF453_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7688954.1"
                     /translation="MADIARLAGVSVATVSRALNGSKLISPETRERIAALARSMNYTI
                     NVGAQNLRLRQNRAIAVIVPFDPDTRQSLSDPFFLSLIGSIADALTARGLEMLLSRAP
                     VGDLEQATQCVDAGRASGIILIGQWHLHQHLNEMARRRVPFVVWGAKMRRQDYATVGG
                     DNRRGGELATAHLLRTGARRIAFLGDDGLPEIGERLEGYLLAHEKAGARPVATLRRAT
                     PFVQSRIVADVEDLVTNGHRFDALFAASDLMALTAVGTLQRLGRRVPEEVQVVGYDDI
                     PLAAFFHPALTTVRQPIAQAGEELVDSLLAQLEGAAPACRLLATELVVRESSRP"
     gene            complement(3620..4519)
                     /locus_tag="VF453_14670"
     CDS             complement(3620..4519)
                     /locus_tag="VF453_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7688955.1"
                     /translation="MRKIDFRNPKHYESIFMLDAVRKSTPFEWKEVYFERREESGVFE
                     TREHRIDGHYLMVKLSPWSIAERCIDGKWHSETQRRGSMAYVPDDCTHRVRYLRPLGA
                     LCLLTLSRQLVDSVAEELNQPVFRGRPRPARDVDLHLLNSVECLDQELRDGNPNGALF
                     AQNFGNMIAAHLVMGYGAATARQRAQQQTLPEKKKKWLDEFIDANLSDAITLDALARQ
                     VGLSPYHFSRLFKNSTGMAPYKYVLHKRIAFAQACLRSDHSSIADIAFACGFNDATQF
                     AKQFRKICGQTPSEYRLASRTGF"
     gene            complement(4612..5778)
                     /locus_tag="VF453_14675"
     CDS             complement(4612..5778)
                     /locus_tag="VF453_14675"
                     /inference="COORDINATES: protein motif:HMM:NF039440.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6421 family protein"
                     /protein_id="HEX7688956.1"
                     /translation="MKTISTLSSLNAIRARTLGLFDQSDAVGPVECRELIDRLNAVPG
                     IRELECAGRLLDDLRRSADGEPFSLRETREYFNAPTSDTLVSLFSAPYARPLGLEYLK
                     FDLLPADPSLARYETNVMPVVVRESTDGLLPTHTVALFPENHVGAIQQPGDKIYYLIN
                     KFAARHHRLTQRIVEHLTTPDSFREIRGATYEQIEKATVYWVWLHEYNHQRVGDLPIP
                     AYLKLKSSRALAGLEELRVDIGSALILLDDAPSFDGKSGFLFEFILAERLLRYGVDGV
                     ALDAEGRANPSYDALSSYMFFNLLKKHRALEVVDGRIRLLGNFVPALRAILAGIRVVE
                     ARIHDAPLEQVKAEMLEFVSQSLETSGSIAHYRHAHYDDMRERLLRLDVEVAMV"
     gene            complement(5775..6398)
                     /locus_tag="VF453_14680"
     CDS             complement(5775..6398)
                     /locus_tag="VF453_14680"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7688957.1"
                     /translation="MTIDIFLAFCIYAVAMSITPGPANFLVLASGARHGVLQSLPVIF
                     GIATGLGAMVLVSGMGLFELLGRSPAFLLALKIVGLAYIAWLAWKIMTSRSAGAPSPD
                     GRAQKVVSYPNGLALPLFNPKAWVMAFGAVTSYTSPGAGGQQVALIALTFLAINAPCM
                     MVWAAFGGFMSRLLTNPRRDLAFKLTMGGLLLLSIVPIAKELLDPSR"
     gene            complement(6433..7644)
                     /locus_tag="VF453_14685"
     CDS             complement(6433..7644)
                     /locus_tag="VF453_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7688958.1"
                     /translation="MTQPRKPPEFATRMQRVQSSAVRDLLKHSKTPGMISLAGGIPAS
                     DLFDLEGLGAIIAGLDARADAGIFQYSETEGERGLRAAIATLLAARGIAAKPDNVLVT
                     SGSQQGLDLAARILLSPGDRIILERPSYLAALQTFGLAEAQVVTAPSDGDGMDVGWVE
                     DYVARQPVKAIYVVPDFGNPSGATLSLERRRRLVELAGRTGTVLLEDDPYGALRFAGA
                     PLPSLQALAAAAGVHEQVIYLSSFSKILAPGLRIGWMVLSDAAFRQAALAKQAIDLHS
                     SSLSQHIVAAYLASGRLGPRLQVLREGYRQRRDALMGALRERLGDAIAFGEPAGGMFL
                     WARFGEAVDSARVLAHGIEQGVVFVPGAAFYAEHPEHNTLRLSYSMITPAAAHEAAER
                     LARALAAAAGE"
     gene            complement(7619..8251)
                     /locus_tag="VF453_14690"
     CDS             complement(7619..8251)
                     /locus_tag="VF453_14690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7688959.1"
                     /translation="MTTSPTPTGRDDRTLRIPLDAAQRALWQARLNTLAVLTHLGAEL
                     DLSGDEVVRIRLVRSLPAHSGGLGGDAINGATLAAMVDCAVATTGVLLFRGRTCGTLQ
                     LSIDFMKPVRTALPELECRVVRRTSTLAFVEARLFGHRGALHLKASGIVCVAKACAGD
                     GESWKSRFLAGTAADADACFLSEPQPGRKAPMALSPVDPNACHDPATQAA"
     gene            complement(8248..9438)
                     /locus_tag="VF453_14695"
     CDS             complement(8248..9438)
                     /locus_tag="VF453_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7688960.1"
                     /translation="MQAQVHLPLQPQPQAQPQPQPQPQPRRVVAARIEALVASDERLA
                     ERLDTLRKFASRVRASEYHLTNACNIRCKGCWFYEYGHDKTAVEAKSLDDWKAFIESQ
                     RRRKVSAALLIGGEPALFPDRVAAFVEGMRYVSISTNGLRVLPREAPFENVTVFISVF
                     GGGPLDDELRAIKPNGRAFTGLFDKALENYQDDPRACFVYAVTERGLAHIEPTVRRIR
                     DNGNTVSFNFYSEYNTGHPLRIHNQRLLLAEMLRVRDAYPDVVLNHPAHIEAMVTGKS
                     WFGSFGRDTCPSISVDHPENRERVQAGEPVLPMFNTWKADLKTLERCCTSGHCEGCRD
                     SQAVYSWFLVSMAKSLESRETLETWIDVAESYWRQFIWSPYRKPRSSAANPESQAPTL
                     VELD"
     gene            complement(9442..10134)
                     /locus_tag="VF453_14700"
     CDS             complement(9442..10134)
                     /locus_tag="VF453_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX7688961.1"
                     /translation="MPLEYRELAIHLMKVHTEGELTGADDYTQVFYNLAPNAYEKLVC
                     CERACEEVEHYQLSAQVLSDLGVDTGYMLEQSFLQRPLYSNELVRGVKTWMERGIFSF
                     LGEAVVLDHLQEFVESSYAPFAEIFTKQIISDEHTHIAHGYRIIREACQTEQGRAQAQ
                     EALDRLWPHLLKLFGRAESPRTKLYIKWGLRKTSNEELRLRFLNKTVPRLQVLGLKVP
                     GTVGEEALLAQE"
     gene            10522..11691
                     /locus_tag="VF453_14705"
     CDS             10522..11691
                     /locus_tag="VF453_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX7688962.1"
                     /translation="MNTAIVGWGHTPFGRLDGRSLEALVVQVAREAVEHAELEFSDID
                     AICLGHFNAGMVPESFCSSLALQADPALRFVPATRLENACASGAAAVFHARALIESGQ
                     ARHVLVIGAEKMTAVDGAAVTGALASASYGPEEARAGLTFPGIFARIARDYFARHGDR
                     SAALARVAVKNHANGARNPLAHLQRALDFDFCNTVSERNPMIAAPLRKTDCSLVSDGA
                     AALVMTDARTALARPRAVRLRAAVHVNDFLPLSRREAIRFEGPALAFSLAYDQARIGV
                     GDLDFAEVHDCFTIAELLSYEAMGLCAPGDGARAIDDGLVLPGGRLPVNLSGGLKAKG
                     HPIGATGVSMHVMAAMQLTGTAGACQHHGAELGAVFNMGGSAVASYVTLLETLKA"
     gene            11773..12585
                     /locus_tag="VF453_14710"
     CDS             11773..12585
                     /locus_tag="VF453_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7688963.1"
                     /translation="MTATSLAHRTALVTGSCQGIGLAIATRLAADGARVILHDLAGST
                     HTREALEAVRAAGAPDACFISADLRAPHEIDGLMDEALGWRDGVDILVNNAGVQFTAS
                     LVDMPPQRWDDILAVNLSSAFHTMRRALPGMIGRGYGRIVNVASVHGMVASVAKAPYV
                     AAKFGLVGLTRVAALECASAGSRESGGITANCIGPGWTETALIEPQIQWRADQFGGDR
                     VAGVRDMLREKQPSLRMSLPSEIGELACMLCQPWAHNITGATIPVDGGWTAQ"
     gene            complement(12634..14181)
                     /locus_tag="VF453_14715"
     CDS             complement(12634..14181)
                     /locus_tag="VF453_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7688964.1"
                     /translation="MFKLEDLGLLTKLVAVAALALLATSATAWWVHRATRTDMNDGIA
                     AVTRYEAKIRDTVAWRGLVDAHLQQVIATAVTGDSTVTQMFASRIKDGAAAIAAKKRD
                     IEAGADSPAEKEALAAVAQRRSDVDKALKKVDDYHSTYETLLAAQAVNKELMPAVEAY
                     FEAINALARVQQRQLDEATAASNARATRNAAAGGLAIVPVAVASIAGVTWLTRSITRP
                     LDHVIHATEAIARGDLTQRIETTRRDEVGRLMQAIGSMNDRLRELVTQVRGGVESVST
                     ASREIASGNSDLSARTERTAANLQQTSAAMAELTRIVVEASRHAEHVNELAGNSARDA
                     QQGGEVVGQVVQSMRGISESSRRIADIIGVIDAIAFQTNILALNASVEAARAGEHGRG
                     FAVVAGEVQSLAHRSADAAKEIKSLIESSGARVEDGVALVERTGRDMTGIVDNICRMR
                     EMVGAIAQAGVAQRDGIQHANAALARVDEMTQQNAALVEESAASAASLREQAQRLAVA
                     VKTFNVG"
     gene            complement(14240..15364)
                     /locus_tag="VF453_14720"
     CDS             complement(14240..15364)
                     /locus_tag="VF453_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7688965.1"
                     /translation="MATLWLAAAAFLGAPGAFAAAPEILIGQVAPLTGVLATTGHQMV
                     VGGRVYFDWVNDHGGVNGARLRTVVADDGYDVAATERATLKLVARPELVALFGFAGTA
                     NVGKLLEDHVLADAGIALVAPYTGGESLRKPFNPWIFHLRAGYSDEADHMVQQLDTLG
                     IHRIALLYQNDGFGLSGKAGVEAALARHGQKLVAAAAYERNTSDVADAVRTILAANAQ
                     AVIMIAVNKPAAVFIKGYRQAGGGGQLFNISVVDPAELVRLAGLDNARGLGISQVVPF
                     PYVPTLPVVREFQAALRQYAPDEAVNYTNFEEFLGAKVLVEGLRRAGAEPTRARLIAA
                     LETLHNFDLGGITVSYGPHERVGSNFVEVTVIGSDGRLLK"
BASE COUNT         2307 a         5335 c         5372 g         2352 t
ORIGIN      
        1 gacgccccgc gcgccaaggc gcagacgctc gacctgacgg aggtggtggt caccggcacc
       61 tcgaccgcga cctcgaagat gaagtccagc gtctcggtga gcacgctgga cggcgaccgc
      121 atcatcgaca accagccgca gaacgccgcc gacgtgctga ccgccatccc ggggctgttc
      181 gtgcagtcca gcggcggtgg cggcaacgcc aacgtgtcgg tgcgcggcct gccgatctcc
      241 gccggcggct cgcgctacct gcagttccag gaggacggcc tgccggtgct gctgttcggc
      301 gacatcgcgt tcggcaaccc cgacgacttc atccgcgtcg accagacgat cgaccgcgtg
      361 gaagccgtgc gcggcggcag cgcctccacg ctgacgacca acggccccgg cggcatcgtc
      421 aactacatca ccaagaccgg cgacgtgccc ggcggcgtgc tggccgtgac cagcggcctg
      481 ggattccagg acgagcgcat cgacttcgca tacggcggca ccctcgcgcc gaagacgaac
      541 tacttcgtcg gcggcttcta cgaatccggc gtgggcccgc gccgtaccgc gggcggcaac
      601 gcggtcgaag gcggccagat caagggcaac gtcacgcgca aggtcgacgc cggctacgtg
      661 cgcctgaact tcaagctgct cgacgaccgc cagccgatgt tcatgcccgg cccggtgaac
      721 gtggtcgacg ggcacatcga cacgatcccg ggtatcgacc cgcgcaagta caccggctac
      781 tcgccggcca tcccggtcga cacggtcctg aacgacgaca acacgtcgac cgtcgtgcac
      841 atgaacgacg gcatgcgcac gcgcagcgcc gccatcggcc tggagacgca cctggacctg
      901 acgcagggct gggtactcga cgacaagttc cgccgcgccg cgaactccgg ccggtgggcg
      961 gcctggtatc ccggcagcgc cgcggcgacg gccgccgccg gcacgagcta cgcgaccggc
     1021 acgaaggcgg gccaggccta ctcgggcctg tccatgacca gcgtcgcctt cgacgtggac
     1081 gtcaaggacc tcggcaacac gaccaacgac ctcaagctga ccaagacgtt cgagcacctc
     1141 gcgggcggcc gtctcgccgt cggcgccggc ctgttcctga acgaccaggc ggtcaacctc
     1201 gtctggaact tcaacggcta cctgacctcg gtctccgagg cgccggtgct gctgaacaac
     1261 gcgacgctgg gcgcgacgca gggcggctcg ctcgggccgg ggttcggcgg ctgctgctcg
     1321 cgcgactacg acggccagta ccacaccgtc gcgccttacg cgttcgcgac cttcgacctt
     1381 ggcgggctga ccgtggacgg cagcgtgcgc gaggacaacc agcacgcctc gggctactac
     1441 aacatcgccc aacccgtcag cgcgacgccc ggcgcggcct tgagctattc gaccgcgacc
     1501 gtccagccca tcgactacgc gctcaagcgc accgagtact cgctcggcgc caactacgcg
     1561 ctgaacaggc agctcgcggt gttcgcccgc tacagcgacg gcgcgtcgtt caacgccgat
     1621 cgcatcatgg acctcggccc gctcgacggc tcggcgtcca tcccggtcaa caccgtcaag
     1681 cagctcgagg gcggcgtgaa ggcgaacctc gggcacgtga gcgcgttcgt gacgctgttc
     1741 gacgccaagg tctcggagtc caactacagc gccacgacgc aggccgccgc cgccaacacc
     1801 tacgacgcga cgggcgtcga gctcgagtcc agctacacga gcggcggcgg ctggtacgtc
     1861 aacgtcggcg cgacctacac cgactcgaag acgaagagct cgaccaacgc cgcgctcgtg
     1921 ggcctgccgg ccaatcgcca gcccaagctg atctaccagg tcggcccggg cttcgagacc
     1981 gaacacgtcg acctcggcgt gcagctcgtg ggcatcggca gctccaagga caccgacacg
     2041 gccaccagcg ccacggtgcc gtacgtgaag ctgccggcct acacgctggt caacggccac
     2101 gcgacctaca acttcgacga ccacacgtcg ttcacgctgg gcttctacaa cctgttcaac
     2161 cgcatcggct ggaccgaggt cgacagcgcc ggcgccgcgc gctcggtgaa cgggcggacg
     2221 ctgcgcggca ccctgcgcta cgcgttctga cgtcgaggcg tcggcccggc cagcggcgcg
     2281 cgggccggcc tctcgcggac ggctcggcgc ctcacgggcg actgctctcg cgcacgacca
     2341 gctcggtggc caggagcctg caggccggcg cggccccctc gagctgcgcc agcagcgaat
     2401 cgacgagctc ctcgccggcc tgggcgatcg gctgccgcac ggtcgtcaac gcgggatgga
     2461 agaaggccgc gagcgggatg tcgtcgtagc ccacgacctg cacctcctcg ggcacgcgcc
     2521 gcccgaggcg ctgcaacgtg cccacggcgg tcagcgccat gaggtcgctg gccgcgaaca
     2581 gcgcgtcgaa gcggtggccg ttggtgacga gatcctcgac gtccgcgacg atgcgcgact
     2641 gcacgaacgg cgtcgcgcgc cgcagcgtcg ccaccggacg cgcgcccgcc ttctcgtgcg
     2701 cgagcaggta gccttcgagg cgctcgccga tctccggcag gccgtcgtcg cccaggaacg
     2761 cgatccgccg cgcaccggtg cgcagcaggt gcgcggtcgc gagctcgccg ccgcggcggt
     2821 tgtcgccgcc gaccgtcgca tagtcctggc ggcgcatctt cgcgccccag accacgaagg
     2881 gaacgcgccg ccgcgccatc tcgttgaggt gctgatggag gtgccattgc ccgatcagga
     2941 tgatgccgct cgcccggccg gcgtcgacgc actgcgtggc ctgctcgagg tcgccgaccg
     3001 gtgctcggga caacagcatc tccagcccgc gcgcggtcag cgcgtcggcg atgctgccga
     3061 tgaggctcag gaagaacgga tcggacaggc tctgccgcgt gtccggatcg aagggcacga
     3121 tcacggcgat ggcgcggttc tgccgcagcc gcaggttctg cgccccgacg ttgatcgtgt
     3181 agttcatcga gcgcgccagg gcggcgatgc gctcgcgcgt ctcggggctg atcagcttgc
     3241 tgccgttcag cgcgcgcgac acggtggcca ccgagacgcc ggcgaggcgg gcgatgtcgg
     3301 ccatttgcgg ccgtgacggc tgggaggcag cggcctcggc gggctcgttg gcgcgggcgg
     3361 gacgacgggg catcggatgg cagcggaacg gggaacgcga ggcggcggct tcgcgatccg
     3421 catcatgcca gacggccggc cggatcgcgg cccaggtcac gtcgcgcggc tgcctgccgc
     3481 cgcggcgcaa tgcgtgccac gcacggacgc cctgacgttc gtagacgatc gcgcgaacat
     3541 ggaggatttc gacgccgcga gatctcgtgc gctgcgtctg gacgcagccg gcgccgcgct
     3601 gacgccgcga accggcctgt cagaagcccg tgcggctcgc cagccggtac tcggacggcg
     3661 tctggccgca gatcttgcgg aactgcttgg cgaattgcgt ggcgtcgttg aacccgcagg
     3721 cgaacgcgat gtcggcgatc gagctgtggt cgctgcgcag gcaggcctgg gcgaaggcga
     3781 tccgcttgtg cagcacgtac ttgtagggcg ccatgccggt cgagttcttg aacagccggc
     3841 tgaagtggta gggactcagg ccgacctgcc tcgcaagcgc atcgagcgtg atcgcgtccg
     3901 agagattggc atcgatgaat tcgtcgagcc acttcttctt cttttccggc agcgtctgtt
     3961 gctgcgcccg ctggcgcgcg gtcgcggcgc cgtatcccat cacgagatgc gccgcgatca
     4021 tgttgccgaa gttctgcgcg aacagcgcgc cattgggatt gccgtcgcgc aattcctggt
     4081 cgaggcactc gacagaattg agcaggtgca ggtcgacgtc cctggccggg cgcggacggc
     4141 cgcggaagac gggctggttc aattcctcgg cgaccgaatc gaccagctgg cgcgacagcg
     4201 tcagcaggca cagcgcgccg agcgggcgca ggtagcgcac gcgatgcgtg caatcgtccg
     4261 gcacgtaggc catgctgccc cggcgttgcg tctcggagtg ccatttgccg tcgatgcagc
     4321 gctcggcgat cgaccacggg ctgagcttga ccatcaggta atgcccgtcg atgcgatgct
     4381 cgcgggtctc gaagacgccg gattcctcgc gccgctcgaa atagacttcc ttccactcga
     4441 acggcgtgct cttgcgcacc gcatcgagca tgaatatcga ttcgtagtgc ttcgggttcc
     4501 tgaagtcgat cttgcgcatg ctggggcttc gtcgtccatc cctgtcgtcc gaatgtactc
     4561 gacgggccgg cgccgatgga agccctgtgc ggggtcgcgt catgcccggc ctcagaccat
     4621 cgcgacctcg acatcgaggc gcagcagccg ctcgcgcatg tcgtcgtaat gcgcgtggcg
     4681 gtaatgcgcg atcgatcccg atgtctccag cgattgcgac acgaattcca gcatctccgc
     4741 cttgacctgc tccagcggcg cgtcgtggat gcgggcctcg accacgcgga tccctgccag
     4801 gatggcccgc agcgcgggga cgaagttgcc gagcaggcgg atccggccgt cgacgacctc
     4861 cagcgcgcgg tgcttcttca gcaggttgaa gaacatgtag ctcgacaatg cgtcgtagga
     4921 cggattcgcg cgaccctcgg cgtcgagggc cacgccgtcg acgccatatc gcagcaggcg
     4981 ctcggcgagg atgaactcga agagaaagcc tgacttgccg tcgaacgaag gcgcgtcgtc
     5041 cagcaggatc agcgcgctgc cgatgtcgac gcgcaattcc tcgagtcccg ccagcgcacg
     5101 gctggacttg agcttcaggt aggccgggat cggcaggtcg cccacgcgct ggtggttgta
     5161 ttcgtgcagc cagacccaat agacggtcgc cttctcgatc tgctcgtagg tcgcgccgcg
     5221 gatctcgcgg aaggaatccg gagtcgtcag gtgctcgacg atgcgttgcg tcagcctgtg
     5281 gtggcgcgcg gcgaacttgt tgatcaggta gtagatcttg tcgcccggct gctggatcgc
     5341 gccgacgtgg ttctccggga acagcgccac cgtgtgcgtc ggcagcaagc cgtccgtcga
     5401 ctcgcgcacg acgaccggca tcacgttggt ctcgtatcgc gcaagcgacg ggtcggccgg
     5461 caggagatcg aacttcaggt actcgaggcc cagcggcctc gcgtacggcg cgctgaacag
     5521 cgagacgagc gtgtcgctcg tcggcgcatt gaagtactcg cgcgtctcgc gcagcgagaa
     5581 cggctcgccg tccgccgacc gccgcaggtc gtccagcagg cggcccgcgc attcgagctc
     5641 gcggataccc gggacggcgt tgagccggtc gatcaattcg cggcattcga ccggcccgac
     5701 cgcatcggac tggtcgaaca gtccgagggt ccgcgcgcgg atcgcattga gggatgacag
     5761 cgtcgagatc gttttcatcg ggacggatcg agtagttctt ttgcgatcgg gacgatggac
     5821 aggagcagca gtccgcccat cgtcaacttg aatgccaggt cgcgccgcgg attggtcagc
     5881 aagcgcgaca tgaatccgcc gaaggccgcc cagaccatca tgcaaggcgc gttgatggcg
     5941 aggaaggtca gcgcgatgag cgcgacctgc tgcccgcctg cccccggcga cgtgtaggac
     6001 gtcaccgcgc cgaaggccat gacccaggcc ttgggattga acagcggcag cgcgagcccg
     6061 ttcgggtagc tcacgacctt ctgggccctg ccgtcggggc tcggggcacc ggcgctgcgc
     6121 gaggtcatga tcttccaggc cagccacgcg atgtaggcga ggcccacgat cttcagcgcg
     6181 agcaggaagg cgggactccg gcccaacagc tcgaacagcc ccatcccgga gaccagcacc
     6241 atcgcgccga ggcccgtcgc gatgccgaag atcaccggca gcgactgcag cacgccgtgc
     6301 cgcgcccccg aggcgaggac caggaaattg gccgggccgg gcgtgatgga catcgccacc
     6361 gcgtagatgc agaacgccag gaagatgtcg atggtcatcg cgtcggggcc ttcgtcggtg
     6421 cgcgccgcat gctcactcgc ccgcggcggc ggcgagcgcc cgcgcgagcc gttccgcggc
     6481 ttcgtgggcg gcggccggcg tgatcatcga ataggacagg cgcagcgtgt tgtgctcggg
     6541 gtgctccgcg tagaaggcgg caccgggcac gaagacgacg ccctgctcga tgccgtgcgc
     6601 cagcacgcgc gcggagtcga ccgcctcgcc gaagcgcgcc cacaggaaca tgccgccggc
     6661 gggctccccg aacgcgatcg catcgccgag gcgctcgcgc agcgcgccca tcagcgcatc
     6721 gcggcgctgg cggtagcctt cgcgaaggac ctgcaggcgc ggccccagcc gcccgctcgc
     6781 gagataggcc gcgacgatgt gctgcgacag cgagctgctg tgcaggtcga tcgcctgctt
     6841 ggcgagcgcg gcctggcgga acgcggcgtc ggacagcacc atccagccga tgcgcagtcc
     6901 cggcgcgagg atcttcgaga acgaggacag gtagatcacc tgctcgtgca cgcccgccgc
     6961 ggccgccagc gcctgcaggc tcggcaatgg tgctcccgcg aagcgcagcg cgccgtacgg
     7021 gtcgtcctcc agcaggacgg tgccggtgcg tcccgcgagc tccaccaggc gccgccggcg
     7081 ctccagcgac agcgtcgcgc cgctgggatt gccgaagtcg ggcacgacgt agatcgcctt
     7141 cacgggctgg cgtgcgacgt agtcctcgac ccagccgacg tccatgccgt cgccgtccga
     7201 gggcgccgtg acgacctggg cctccgccag cccgaaggtc tgcagcgcgg cgaggtagga
     7261 cggccgttcg aggatgatgc ggtcgccggg cgacagcagg atgcgcgcgg ccaggtccag
     7321 gccctgctgc gacccggagg tcacgaggac gttgtccggc ttggcggcga tgccgcgcgc
     7381 ggccagcagg gtcgcgatcg cggcgcggag tccgcgctcg ccttcggtct cgctgtactg
     7441 gaagatgccg gcatcggcgc gcgcatcgag gccggcgatg atcgcgccga ggccttcgag
     7501 gtcgaacagg tcggaggccg gaatgccgcc ggccagcgag atcatgccgg gcgtcttgct
     7561 gtgcttgagc aggtcgcgca ccgcggagct ttgcacgcgc tgcatgcggg tggcgaattc
     7621 aggcggcttg cgtggctggg tcatggcagg cgttcggatc gacaggggaa agggccatcg
     7681 gcgctttgcg cccgggttgg ggttcggaca ggaagcaggc gtcggcgtcg gccgccgttc
     7741 ccgccaggaa gcgggatttc cagctctcgc cgtcgcccgc gcaggccttc gcgacgcaga
     7801 cgatcccgct ggccttgaga tgcaacgcgc cgcgatggcc gaacagccgc gcctcgacga
     7861 aggccagcgt gctcgtgcgc cgcacgacgc ggcattcgag ctccggcagc gcggtgcgaa
     7921 cgggcttcat gaaatcgatg gacagctgca gcgtgccgca ggtgcggccg cggaacagca
     7981 gcacgccggt ggtcgcgacg gcgcagtcga ccatcgccgc gagcgtcgcg ccgttgatcg
     8041 cgtcgccgcc caggccaccg gaatgcgcgg gcagcgagcg gacgaggcgg atccgcacca
     8101 cctcgtcgcc ggacaggtcc agttccgcgc ccaggtgcgt cagcacggcc agcgtgttga
     8161 ggcgggcctg ccagagagcg cgctgcgcgg cgtccagtgg aatccggagg gtgcggtcgt
     8221 cccgtccggt cggcgtcggc gaggtcgtca atcgagttcc accagcgtcg gtgcctgcga
     8281 ctcggggttg gccgccgaag aacgcggctt gcggtacggc gaccagatga actggcgcca
     8341 gtagctttcc gccacgtcga tccaggtttc cagcgtctcg cgcgactcga gcgacttggc
     8401 catgctgacg aggaaccagc tgtagacggc ctggctgtcg cggcagccct cgcaatgtcc
     8461 cgaggtgcag cagcgctcga gcgtcttgag gtccgccttc caggtgttga acatcggcag
     8521 cacgggctcg ccggcctgca cgcgttcccg gttctcggga tggtcgacgc tgatgctggg
     8581 gcaggtgtcc cgcccgaagc tgccgaacca gctcttgccc gtcaccatcg cctcgatgtg
     8641 cgcggggtgg ttgagcacga cgtcgggata cgcgtcgcgc acgcgcagca tctcggccag
     8701 cagcaggcgc tggttgtgga tgcgcagcgg atgcccggtg ttgtattcgc tgtagaagtt
     8761 gaacgacacc gtgttgccgt tgtcgcggat gcggcggacc gtcggctcga tgtgcgcgag
     8821 cccccgctcc gtcaccgcgt agacgaagca cgcgcgcggg tcgtcctggt agttctcgag
     8881 cgccttgtcg aagagcccgg tgaacgcgcg cccgttgggc ttgatcgcgc gcaactcgtc
     8941 gtccagcggg ccgccgccga agaccgagat gaacacggtg acgttctcga acggcgcctc
     9001 gcgcggcagc acgcgcagcc cgttcgtcga gatcgacacg tagcgcatcc cttcgacgaa
     9061 cgcggcgacg cgatccggga acagcgccgg ctccccgccg atcagcaggg ccgcggacac
     9121 cttcctgcgg cgctgcgact cgatgaacgc cttccagtcg tcgagcgact tggcctccac
     9181 ggcggtcttg tcgtggccgt actcgtagaa ccagcagccc ttgcagcgga tgttgcaggc
     9241 attggtcagg tggtactcgg acgcgcgcac gcgcgaggcg aacttgcgca gcgtgtccag
     9301 gcgttcggcg aggcgctcgt cgctggcgac cagcgcttcg atgcgcgccg cgacgacgcg
     9361 gcgcggttgc ggctgcggct gcggctgcgg ctgcgcctgc ggttgcggct gcagcgggag
     9421 gtggacttgg gcctgcatcg gtcactcctg cgccagcagc gcttcctcgc ccacggtgcc
     9481 gggaaccttg agcccgagca cctgcaggcg cggcaccgtc ttgttgagga agcgcaggcg
     9541 caactcctcg ttgctggtct tgcgcaggcc ccacttgatg tagagcttcg tgcgcggcga
     9601 ctcggcgcgg ccgaagagct tgagcaggtg cggccagagc cggtcgagcg cttcctgcgc
     9661 ctgcgcgcgt ccctgctcgg tctggcaggc ctcgcggatg atccggtagc cgtgcgcgat
     9721 atgcgtgtgc tcgtcgctga tgatctgctt ggtgaagatc tccgcgaagg gcgcgtagct
     9781 ggattcgacg aactcctgga gatggtccag gacgaccgcc tcgccgagga acgagaatat
     9841 gccgcgctcc atccaggtct tgacgccgcg gacgagttcg ttggaataga gcgggcgctg
     9901 caggaaactc tgttccagca tgtagccggt atccacgccg aggtcggaca gcacttgcgc
     9961 gctgagctgg taatgctcga cctcctcgca ggcgcgctcg cagcagacca gcttctcgta
    10021 ggcgttcggc gcgaggttgt agaagacctg cgtgtagtcg tccgcgccgg tcaactcgcc
    10081 ctcggtatgc accttcatca ggtggatcgc cagctcgcga tattcgagcg gcatcttgtg
    10141 gaaatcctcg ggcgacctga ccgatccggg tttcgcgaga acctcgatgg ccgattcgct
    10201 cgcgtgaaga tccattctca agatatttct ccgttcatcg tggatgccgt cgggcaagtc
    10261 taggaagccg ggtgcgcgcc gaggcgtgca aatccggcgg cctggtgttt tcttgccatc
    10321 aactaaacgg atgaaatgta cgaattcgat ggcggacggc tatccctcct cggtgggagc
    10381 ggacggcgtc gcgaacgcac gtcgcacctg ccgtccgttg atggaattcc atacgggttg
    10441 ccactgatgc gattgcacgt cttgtgacgg gcgagggggc cgaaatacgc tgcgctccgt
    10501 tgccatgcag gagtgcacgc catgaacacc gcgatcgtcg gctggggtca caccccgttc
    10561 ggaagactgg acggccgcag cctggaggcg ctcgtcgtcc aggtcgcgcg cgaggccgtc
    10621 gagcacgcgg agctcgaatt cagcgacatc gacgcgatct gcctgggcca tttcaacgcg
    10681 ggcatggtgc ccgagtcgtt ctgcagctcg cttgcgctgc aggccgatcc cgccctacga
    10741 ttcgtgccgg cgacgcgcct tgaaaacgca tgcgcgtccg gcgcggccgc ggtcttccat
    10801 gcccgcgccc tgatcgagtc cgggcaggcg cggcacgtgc tcgtcatcgg cgcggagaag
    10861 atgaccgcgg tcgacggcgc cgcggtgacg ggcgcgctcg cgagcgcgag ctacggcccg
    10921 gaggaggcgc gcgcgggcct gaccttcccg ggcatcttcg cgcgaatcgc gcgcgactac
    10981 ttcgcgcgcc atggcgaccg ctcggccgcc ctcgcccgcg tcgcggtgaa gaaccacgcg
    11041 aacggtgcgc gcaacccgct ggcccacctc cagcgcgccc tcgacttcga tttctgcaac
    11101 acggtgtccg agcgcaatcc gatgattgcc gcgccgctgc gcaagaccga ctgctcgctg
    11161 gtgtcggacg gcgcggcggc cctggtgatg acggacgcgc gcacggcgct ggcgcgcccg
    11221 cgcgcggtgc ggctgcgcgc cgcggtgcac gtcaacgact tccttccgct gtcgcgccgc
    11281 gaggccatcc gcttcgaggg ccccgcgctc gcgttctcgc tggcctacga ccaggcgcgc
    11341 atcggcgtcg gcgacctgga cttcgccgag gtgcacgact gcttcaccat tgcggagctc
    11401 ctgagctatg aggcgatggg tctgtgcgca cccggcgacg gcgcgcgcgc gatcgacgac
    11461 gggctcgtcc tgccgggcgg acgcctgccg gtgaacctgt ccggcggcct gaaggcgaag
    11521 ggccacccga tcggcgcgac cggcgtgtcc atgcacgtga tggcggcgat gcagctgacc
    11581 ggcaccgccg gcgcctgcca gcaccacggt gccgaactcg gggccgtctt caacatgggc
    11641 ggctccgcgg tggcgtcgta cgtcacgctg ctcgagaccc tcaaggcatg acctccccgg
    11701 gcgaaccgga tcggccgcgc ccgcgttgcg aacctctcac ccgatccccc acgcaatcca
    11761 tcgtccaatg acatgaccgc tacctccctc gcccatagaa ccgccctggt caccgggtcc
    11821 tgccagggca tcggcctggc catcgcgacc cggctcgcgg ccgacggcgc ccgcgtcatc
    11881 cttcacgatc tcgcaggctc gacgcacacc cgggaggcgc tggaggccgt gcgcgcggcg
    11941 ggcgcgcccg acgcgtgctt catctccgcc gacctgcgcg cgccccacga gatcgacggc
    12001 ctcatggacg aagcgctggg ctggcgcgac ggcgtggaca tcctggtcaa caacgccggg
    12061 gtgcagttca cggcgtccct cgtcgacatg ccgccgcagc gctgggacga catcctcgcg
    12121 gtgaacctgt cctccgcgtt ccacacgatg cggcgcgccc tgcccggcat gatcgggcga
    12181 ggctacggcc ggatcgtcaa cgtcgcctcg gtgcacggga tggtcgcttc cgtggcgaag
    12241 gcgccctacg tcgccgcgaa gttcgggctg gtcgggctca cgcgcgtcgc ggcgctcgaa
    12301 tgcgcgtcgg cgggcagccg cgagtcgggc gggatcaccg ccaactgcat cggcccgggc
    12361 tggacggaga cggccctgat cgagccgcag atccagtggc gcgccgacca gttcggcggc
    12421 gaccgcgtcg caggcgtgcg cgacatgctg cgcgagaagc aaccgagcct gcggatgtcg
    12481 ctgccctcgg agatcggcga gctcgcctgc atgctctgcc agccctgggc gcacaacatc
    12541 accggggcga cgatccccgt ggacggcggc tggacggcgc agtgagcgcg ggcgggccgt
    12601 caccgcgggg cggcctgcgc cgcgcccgcg ggctcagccc acgttgaacg tcttcacggc
    12661 cacggccagc cgttgggcct gttcgcgcag cgacgccgcg ctggccgccg attcctccac
    12721 cagcgccgcg ttctgctgcg tcatctcgtc gacccgcgcc agcgccgcgt tcgcgtgctg
    12781 gatgccgtcg cgctgcgcca cccccgcttg cgcgatcgcg ccgaccatct cgcgcatgcg
    12841 gcagatgttg tcgacgatgc cggtcatgtc gcggccggtg cgttccacca gcgcgacgcc
    12901 gtcctcgacg cgggcgccgg acgactcgat cagcgacttg atctccttgg ccgcgtcggc
    12961 gctgcggtgc gcgagcgact gcacctcgcc ggccacgacg gcgaagccgc ggccgtgctc
    13021 gccggcgcgc gccgcctcga cggaggcgtt cagcgcgagg atgttcgtct ggaacgcgat
    13081 cgcgtcgatc acgccgatga tgtccgcgat tcgacgcgag ctctccgaga tcccgcgcat
    13141 gctctggacg acctggccga cgacctcgcc gccctgctgc gcgtcgcggg ccgagttccc
    13201 ggcgagctcg ttgacatgct ccgcatggcg cgaagcctcg accacgatgc gcgtcagctc
    13261 ggccatcgcg gcggaggtct gctgcaggtt cgcggcggtg cgctcggtgc gcgcggagag
    13321 gtcgctgttg ccgctcgcga tctcgcgcga cgccgtggac accgattcga ccccgcctcg
    13381 cacctgcgtc acgagctcgc gcaggcggtc gttcatgctg ccgatggcct gcatcaggcg
    13441 gccgacctcg tcgcggcgcg tcgtctcgat gcgctgcgtg aggtctccgc gcgcgatcgc
    13501 ctcggtcgca tggatgacgt ggtccagggg ccgcgtgatc gagcgcgtca gccaggtcac
    13561 gccggcgatg ctggcgacgg ccaccggcac gatggccagg ccgcccgcgg ccgcgttgcg
    13621 cgtggcgcgc gcgttggacg ccgccgtcgc ctcgtcgagc tggcgctgct gcacgcgcgc
    13681 cagcgcgttg atcgcctcga agtacgcctc caccgccggc atcagctcct tgttgaccgc
    13741 ctgcgccgcg agcagcgtct cgtaggtgga gtggtagtcg tcgaccttct tgagcgcctt
    13801 gtcgacgtcg ctgcgtcgct gcgccaccgc cgcgagcgcc tccttctccg ccggcgaatc
    13861 ggcgcccgcc tcgatgtcgc gcttcttggc cgcgatcgcc gccgcgccgt ccttgatgcg
    13921 gctcgcgaac atctgcgtca cggtggaatc gcccgtgacc gcggtcgcga tcacctgctg
    13981 caggtgcgca tcgaccagcc cgcgccaggc caccgtgtcg cggatcttgg cctcgtagcg
    14041 cgtgacggcg gcgatgccgt cgttcatgtc cgtgcgggtc gcgcgatgca cccaccaggc
    14101 cgtcgcggag gtggccagca gcgccagggc cgcgacggcg acgagcttgg tcagcaggcc
    14161 caggtcttcg agcttgaaca tggagatgtc ccgatgcgtg tggtggttga acggcggacg
    14221 ggcgcggcgt cgtccggcgt cacttgagga gtcggccgtc gctgccgatc acggtgacct
    14281 cgacgaagtt ggagccgacc cgctcgtgcg gcccgtagct cacggtgatg ccgccgaggt
    14341 cgaagttgtg cagcgtctcc agggcggcga tcaggcgcgc ccgggtcggc tcggcgcccg
    14401 ctcgccgcag gccctcgacg agcaccttgg cgccgaggaa ttcctcgaag ttcgtgtagt
    14461 tgacggcctc gtcgggcgcg tattggcgaa gggcggcctg gaactcgcgc acgaccggca
    14521 gcgtcggcac gtacggaaag ggcacgacct ggctgatgcc cagcccgcgc gcgttgtcca
    14581 ggcccgcgag ccgcacgagc tcggcggggt cgacgaccga gatgttgaac agctggccgc
    14641 cgccgccggc ctgccggtag cccttgatga agacggcggc cggcttgttg acggcgatca
    14701 tgatgaccgc ctgcgcgttg gccgccagga tcgtgcgcac ggcgtcggcg acatcgctcg
    14761 tgttgcgttc gtaggctgcc gccgccacca gcttctggcc atgccgcgcg agggcggcct
    14821 cgacgccggc cttgcccgaa agcccgaaac cgtcgttctg gtacagcaac gcgatgcgat
    14881 ggatgccgag ggtgtcgagc tgctgcacca tgtggtcggc ctcgtccgag tagccggcgc
    14941 gcaggtggaa gatccacgga ttgaacggct tgcgcagcga ctcgccgccg gtgtagggcg
    15001 ccaccagcgc gatgcccgca tcggcgagca cgtggtcctc gaggagcttg cccacgttgg
    15061 cggtccccgc gaagccgaac agcgcgacga gctcgggtct cgcgaccagc ttgagggtcg
    15121 cgcgctccgt ggcggcgacg tcgtagccgt cgtcggcgac gacggtgcgc aggcgggcgc
    15181 cgttgacccc gccgtggtcg ttgacccagt cgaagtacac gcggccgccg accaccatct
    15241 ggtgccccgt ggtcgcgagc acgccggtca gcggcgcgac ctgcccgatc aggatctccg
    15301 gcgcggcggc gaaggcgccc ggtgcgccga ggaacgccgc ggccgcgagc cagagcgtgg
    15361 ccagga
//
LOCUS       DASVLN010000613         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_302249, whole genome shotgun sequence.
ACCESSION   DASVLN010000613 DASVLN010000000
VERSION     DASVLN010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302249"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /gene="rsgA"
                     /locus_tag="VF453_14725"
     CDS             <1..717
                     /gene="rsgA"
                     /locus_tag="VF453_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX7688966.1"
                     /translation="SLLKRQDEWRSKSFAANIDLLLVMVATDPPYSESQLARALIAAE
                     DGGIAVRIVLNKVDLPEAGAARTRLAPYRAMDYEVVELALKADPAAARARLVPMLEGK
                     TTLVLGPSGMGKSTLVNLLVPHADAQVGEISKALKSGKHTTTTTTWYWLDDERRSALI
                     DSPGFQEFGIHHIEAPALASHMRDYRRWLGDCRFANCTHRHEPACGVRAAIARGDITP
                     SRERIYEELMGELERAAGRH"
     gene            complement(845..1441)
                     /locus_tag="VF453_14730"
     CDS             complement(845..1441)
                     /locus_tag="VF453_14730"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HEX7688967.1"
                     /translation="MSLPLTPPGAFFLASAVACQAIRVYFAARARRAGGGAKFSKAPA
                     WDRVLIALVGMGQFVLPAAFLFTPWLDGADVVLPAASLPLGAAVTLAGLGLFWRAHAD
                     LGESWSVTLELNREHRLVTRGVYRAMRHPMYASFLVLGLGQGLLLANWLAGWAGLASV
                     ALMVAVRLPREEAMMVGRFGDEYRAYRARTGAVWPRRP"
     gene            complement(1488..2480)
                     /locus_tag="VF453_14735"
     CDS             complement(1488..2480)
                     /locus_tag="VF453_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HEX7688968.1"
                     /translation="MSFFAVLLALLLEQVKPLPRRNVVHDSLASWVGWTGRNFDAGKP
                     QHARIVWLVTVIAPALLSALVYVGIRHYSVFAALGFDVAVLYLTLGFRQFSHYFTDIR
                     AALESGDELEARQLLAEWRHLDASELPRTELLRHMIEHSLLAAHRHVFGVFFSFVVFS
                     AIGLGPAGAVLYRMAEFASRYWAFRSKALDTPINENLMTLSQRLFSAIDHVPARLTAF
                     GFAVVGNFQDAVDGWKRDARLWKHANEGIILAAAAGAVGVQLGGGAAPGVTPDRSKTF
                     TSGEGVAAADAEGSTTQGLPPQLGHLGIVVGLVWRSVILWMLLVAILKIANLTA"
     gene            complement(2559..>3105)
                     /locus_tag="VF453_14740"
     CDS             complement(2559..>3105)
                     /locus_tag="VF453_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX7688969.1"
                     /translation="FRGDGGRFADRAPRPAAVLVPLVRRPAGLAVLLTRRTDHLNDHA
                     GQVSFPGGRTDPEDVDAVATALREAREEVGIHADEVEVIGRLPTYTTVTAYEVTPVVG
                     LLEPPRALALDAFEVAEVFEVPLAFLMDPAHHRHHEVQLGDAARRFLSMPWAREPGCG
                     EYFIWGATAAMLRNMYRFLAR"
BASE COUNT          499 a         1113 c         1133 g          360 t
ORIGIN      
        1 agcctgctca agcgccagga cgaatggcgc agcaagagct tcgccgccaa tatcgacctg
       61 ctgctggtga tggtcgcgac cgatccgccg tacagcgaat cgcagctcgc gcgcgcgctg
      121 atcgcggccg aggacggcgg catcgccgtg cgcatcgtgc tcaacaaggt cgacctcccc
      181 gaggccgggg ccgcgcggac gcggctcgcg ccctatcgcg cgatggacta cgaggtggtc
      241 gagctcgcgc tcaaggccga tccggcggcg gcgcgcgcgc ggctcgtccc catgctcgag
      301 ggcaagacga cgctcgtcct gggcccgagc ggcatgggca agagcacgct cgtgaacctg
      361 ctggtgccgc acgcggacgc gcaggtggga gagatctcga aggcgctgaa gtccggcaag
      421 cacacgacga ccacgacgac ctggtactgg ctggacgacg agcgccgcag cgcgctcatc
      481 gactcgccgg gcttccagga gttcggcatc caccacatcg aggcgcccgc gctggcctcg
      541 cacatgcgcg actaccgacg ctggctcggc gactgccgtt tcgccaactg cacccaccgc
      601 cacgaacccg cctgcggcgt gcgcgcggcc atcgcgcgcg gcgacatcac gccgtcgcgc
      661 gagcggatct acgaggagct gatgggcgag ctggagcggg cggccggccg gcactgagcg
      721 agcgtcgccc cgtcgcgacc cgtcgcatga cgccgatgcg gccgcgtgct cgcccggtcg
      781 ccaccggccc cgcgacgtcc gcggcgacga cgttcgacgc atcgccgcgc caggagcgtc
      841 tcggctaggg ccgtctaggc cagaccgcac ccgtgcgcgc ccggtaggcc cggtactcgt
      901 cgccgaagcg cccgaccatc atcgcctcct cgcgcggcag ccgcaccgcg accatcagcg
      961 cgaccgacgc gagcccggcc cagccggcga gccagttggc gagcagcagg ccctggccga
     1021 ggcccagcac gaggaaggac gcatacatcg gatggcgcat cgctcggtag acgccgcggg
     1081 tgacgagccg gtgctcgcga ttgagctcca gcgtgaccga ccagctctcg cccaggtcgg
     1141 cgtgcgcgcg ccagaacagc cccagcccgg cgagcgtgac cgctgcgccg agcggcaagg
     1201 aggcggccgg cagcacgacg tccgcgccat ccagccaggg cgtgaacagg aatgcggccg
     1261 gcagtacgaa ctgacccatc ccgacgagcg cgatcagcac gcgatcccag gcgggcgcct
     1321 tggagaactt cgccccgccg ccggcgcgcc gcgcacgtgc ggcgaagtac acgcggatcg
     1381 cctggcaggc gaccgccgag gcgaggaaga acgcgccggg cggcgtcagc ggcaggctca
     1441 tcgcgggagc gccatcggca gcgggatcgg cggacccggc cgcgtcttca ggcggtcagg
     1501 ttggcgatct tgaggatggc caccagcaac atccacagga tcaccgaccg ccacacgagc
     1561 ccgacgacga tgcccaggtg gccgagctgc ggcggcaggc cctgggtcgt gctgccctcg
     1621 gcgtcggcgg cggcgacgcc ctcgccgctc gtgaaggtct tggagcggtc gggcgtgacc
     1681 ccgggcgccg cgccgccgcc cagctgcaca cccaccgcgc cggcggcggc ggcgaggatg
     1741 atgccctcgt tggcatgctt ccacagccgc gcgtcgcgct tccagccgtc gacggcgtcc
     1801 tggaagttgc cgaccacggc gaagccgaac gcggtcaggc gcgcgggcac gtggtcgatc
     1861 gcgctgaaca ggcgctgcga cagcgtcatc aggttctcgt tgatcggcgt gtccagcgcc
     1921 ttgctgcgga aggcccagta gcgcgacgcg aactccgcca tccggtacaa cacggcgccg
     1981 gcgggaccga ggccgatcgc cgagaacacg acgaagctga agaacacgcc gaacacgtgg
     2041 cggtgcgcgg cgagcagcga gtgctcgatc atgtggcgca gcagctcggt gcgcgggagc
     2101 tccgaggcgt cgagatgacg ccactcggcc agcagctggc gcgcctcgag ctcgtcgccg
     2161 ctctcgagcg cggcgcggat gtcggtgaag tagtggctga actgccggaa gccgagcgtc
     2221 aggtacagca ccgcgacgtc gaagcccagc gccgcgaaca cgctgtagtg gcggatgccg
     2281 acgtacacga gcgccgacag cagcgccggc gcgatcacgg tcaccagcca gacgatgcgc
     2341 gcgtgctgcg gcttgccggc gtcgaagttg cggcccgtcc agccgaccca ggaggccagt
     2401 gaatcgtgga cgacgttgcg gcgcggcagc ggcttgacct gttcgagcag cagggcgagg
     2461 aggacggcga agaaactcat cgccgcgaat gatagcccgt ggtgacgagc ggcccgcgtg
     2521 cacgcggcgc cggatttcac gcacggcgcg cagccgcttc agcgcgcgag gaagcggtac
     2581 atgttgcgca acatcgccgc cgtcgcgccc cagatgaaat actcgccgca gccgggctcc
     2641 cgcgcccacg gcatggacag gaagcggcgc gcggcgtcgc ccagctggac ctcgtggtgg
     2701 cgatgatgcg cggggtccat caggaacgcg agcggcacct cgaacacctc ggcgacctcg
     2761 aaggcgtcga gcgccagcgc gcgcggcggc tcgagcaggc cgaccaccgg cgtcacctcg
     2821 taggcggtga cggtcgtgta cgtcggcagg cggccgatca cctcgacctc gtcggcgtgg
     2881 atgcccacct cctcgcgcgc ctcgcgcagc gcggtggcga cggcatcgac gtcctcggga
     2941 tcggtgcggc cgccgggaaa gctcacctgg ccggcgtggt cgttgagatg gtcggtgcgg
     3001 cgggtgagca ggacggccag gccggccggc cgcctcacga gcggcacgag cacggcggcc
     3061 ggccgcggcg cgcgatcggc gaagcggccg ccgtcgccgc ggaat
//
LOCUS       DASVLN010000614         5083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_246536, whole genome shotgun sequence.
ACCESSION   DASVLN010000614 DASVLN010000000
VERSION     DASVLN010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246536"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1176)
                     /locus_tag="VF453_14745"
     CDS             complement(73..1176)
                     /locus_tag="VF453_14745"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7688970.1"
                     /translation="MEFIDLKTQYAALREEIGARMQKVLDHGQYIMGPEVKELEAALA
                     ADTGAKHCVTVASGTEALLIALMALDLKPGDEVVTTPFTFAATAETIVLLGGRPVWVD
                     IEPDTCNIDAAKIEAAITPRTKAIIPVSLYGQVADMDEINAIGARHGVAVIEDAAQSY
                     GASYKGRRSNALSTFGCTSFFPSKPLGCYGDGGAIFTSDDALAQAAREIRVHGQSARY
                     THTRVGVGGRMDTLQCAVLLAKLPRLAWELGRRRELGDRYHAALAGLDVTTLTVRPDR
                     DSVWGQYTVRVKDRARVQAALAEAGVPTAVHYPRPLHRQPAYESFGDPDACPNAIAAA
                     AEVMSLPMSPDLADADFDRVVAALAAAVGPREK"
     gene            complement(1217..1795)
                     /locus_tag="VF453_14750"
     CDS             complement(1217..1795)
                     /locus_tag="VF453_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX7688971.1"
                     /translation="MGTTIHPTAIVDEGAVLGDGCRVWHWVHISAGARIGRGCSFGQG
                     VFVGNDVAIGDNVKIQNNVSVYDAVTLEDDVFCGPSMVFTNVYNPRSAVVRKDEYRRT
                     RVRRGATLGANATIVCGTTIGRHAFVGAGAVVQKDVPDFALVVGVPARQVGWMSRHGE
                     RLPLPLAGEGEAACPHTGDRYRLEGRVLSLID"
     gene            complement(1804..2883)
                     /locus_tag="VF453_14755"
     CDS             complement(1804..2883)
                     /locus_tag="VF453_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7688972.1"
                     /translation="MSTHSLRPHSAPPVTDRKVRFALVGCGRIAANHMESMKVHAERA
                     ELVAVCDTDAAKLAAAVARTGATGYRSLRELLDAETPDCVVVTTPSGLHSQQVMEVAA
                     RGIDVMTEKPMATRWQDGLAMVRACDEAGVRLFVVKQNRRNRTLQLLKQAMDAGRFGR
                     IYSVAVNVFWTRPQEYYDSAAWRGTWEFDGGAFMNQASHYVDLLDWVVGPVESVMAYT
                     GTLARNIEVEDTGVAALKWRNGAMGTLNVTMLTYPKNLEGSITILGEKGSVRVGGVAV
                     NEIQHWQFSQPHEMDAHIADASYQTTSVYGFGHPLYYDNVISTLRGEAQPETDGREGL
                     KSLELLIAMYLSSRDGKRVNLPLEY"
     gene            3058..4575
                     /gene="lysS"
                     /locus_tag="VF453_14760"
     CDS             3058..4575
                     /gene="lysS"
                     /locus_tag="VF453_14760"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX7688973.1"
                     /translation="MDTTTPSASPALPLDENKLVAERREKLAAIRARGGVAFPNDFKP
                     KDHACDLQHKYGKCTNDELEPRAVAVTVAGRMMLKRTMGKAAFCTLQDATGRIQLRVA
                     IDQVGEELYGQFKHWDLGDILGAEGTLFVTKTGELTILVKTLRLLTKSLRPLPDKFHG
                     MTDQEQKYRQRYVDLITDEDARKRFIARSKALGSIRGYMLEHGFLEVETPMLHPIPGG
                     ANAKPFTTHHNALDQEMFLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEF
                     TMMEFYAAYWNHHDVMDFTEAVLRHAARAATGSASLTYAGKEVHLDKPFERLSVRDSL
                     VRYAGLAEEQTRDAAVLRERILAAGEEAPAHWTIAELQFGLFEAVVEAQLWQPTFIID
                     YPVEVSPLARASDADPTITERFELFITGREYANGFSELNDAEDQAARFQAQVANKDAG
                     DEEAMYYDADFIRALEYGMPPTGGCGIGIDRLMMLLTDSPTIRDVILFPALRREA"
     gene            4675..>5083
                     /locus_tag="VF453_14765"
     CDS             4675..>5083
                     /locus_tag="VF453_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX7688974.1"
                     /translation="MTARLQHLDEIEQCIWSELERCVAPRPPREADEGREPHDLRVAA
                     LETEDAPGDGDSPGVVLREVDAPERRLVFYTDARAPEVRRLESHPEGTLTFRSRRLGW
                     QLRMRVRLAVERDGLAVSSRWARLQTRAGADDYR"
BASE COUNT          740 a         1831 c         1763 g          749 t
ORIGIN      
        1 ccgccggagc ctccgacgcg gccggcgcgt cgggcgatcc cgacgtgccg gggacgggca
       61 ccggcgcggc gctcacttct cgcgcggccc cacggcggcg gcgagcgcgg ccaccacgcg
      121 gtcgaagtcg gcgtccgcca ggtcgggact catcggcagg ctcatcacct cggcggccgc
      181 ggcgatcgcg ttcgggcagg cgtccgggtc gccgaaggac tcgtaggccg gctggcgatg
      241 cagcgggcgc gggtagtgca ccgcggtcgg cacgccggcc tcggcgagcg cggcctgcac
      301 ccgcgcgcga tccttcacgc gcaccgtgta ctggccccag acgctgtcgc ggtcgggacg
      361 cacggtcagc gtcgtgacgt ccaggccggc gagcgccgcg tggtagcggt cgccgagttc
      421 gcgccggcgc ccgagctccc acgccaggcg cggcagcttg gccagcagca cggcgcactg
      481 cagcgtgtcc atgcggccgc cgacgcccac acgggtatgg gtataacgcg cgctctgccc
      541 gtgcacgcgg atctcgcgcg ccgcctgggc gagcgcgtcg tcgctcgtga agatggcccc
      601 gccgtcgccg tagcagccga gcggcttgct cgggaagaag ctcgtgcacc cgaaggtcga
      661 cagcgcattg ctgcgccggc ccttgtagct cgcgccgtag ctctgcgccg cgtcctcgat
      721 caccgccacg ccgtgccgcg cgccgatcgc gttgatctcg tccatgtcgg cgacctggcc
      781 gtacaggctc accgggatga tcgccttcgt gcgcggcgtg atcgcggcct cgatcttcgc
      841 cgcgtcgatg ttgcaggtgt cgggctcgat gtcgacccac accggccggc cgcccagcag
      901 cacgatggtc tcggccgtcg cggcgaaggt gaacggcgtc gtgacgacct cgtcgcccgg
      961 cttcaggtcg agcgccatca gcgcgatcag cagcgcctcg gtgccgctcg cgaccgtgac
     1021 gcagtgcttc gcgccggtgt cggcggcgag cgcggcctcg agctccttga cctcggggcc
     1081 catgatgtac tgcccgtggt ccagcacctt ctgcatgcgc gcgccgatct cctcgcgcag
     1141 cgcggcgtac tgcgtcttca ggtcgatgaa ctcgatcttc ttcggggtgg cggcagcggt
     1201 cacgggagtc tttcgttcaa tcgatcagcg agagcacgcg gccctcgagc cggtagcggt
     1261 cgccggtgtg cgggcaggcg gcctcgccct cgcccgccag cggcagcggc aggcgctcgc
     1321 cgtggcggct catccagccg acctggcgcg cgggcacgcc gacgaccagc gcgaagtccg
     1381 gcacgtcctt ctggacgacc gcgcccgcgc cgacgaaggc gtggcggccg atggtcgtgc
     1441 cgcagacgat ggtggcgttc gcgcccagcg tcgcgccgcg gcgcacccgc gtgcgccggt
     1501 actcgtcctt gcgcacgacg gccgaacgcg ggttgtagac gttggtgaag accatgctcg
     1561 ggccgcagaa gacgtcatcc tccagcgtca cggcgtcgta gaccgagacg ttgttctgga
     1621 tcttgacgtt gtcgccgatc gcgacgtcgt tgcccacgaa cacgccctgc ccgaacgagc
     1681 agccgcgccc gatccgcgcg ccggcgctga tgtgcaccca gtgccagacg cggcagccgt
     1741 cgcccaggac ggcgccttcg tcgacgatcg cggtcggatg gatggtcgtg cccatacggg
     1801 gtatcagtat tccaacggca ggttcacgcg cttgccgtcg cgcgaggaca ggtacatcgc
     1861 gatcagcagc tcgagcgact tcaggccttc gcggccgtcc gtctcgggct gggcctcgcc
     1921 gcgcagcgtg gagatgacgt tgtcgtagta cagcggatgg ccgaagccgt agaccgaggt
     1981 cgtctggtag ctcgcgtcgg cgatgtgggc gtccatctcg tgcggctgcg agaactgcca
     2041 gtgctggatc tcgttgaccg cgacgccgcc gacgcgcacg ctgcccttct cgccgaggat
     2101 cgtgatcgag ccctcgaggt tcttcgggta ggtgagcatc gtcacgttga gcgtgcccat
     2161 cgcgccgttg cgccacttca gcgcggcgac gccggtatcc tccacctcga tgttgcgcgc
     2221 cagcgtcccg gtgtaggcca tcacgctctc gaccgggccg accacccagt ccagcaggtc
     2281 gacatagtgg ctcgcctggt tcatgaacgc gccgccgtcg aactcccacg tgccccgcca
     2341 ggcggccgag tcgtagtact cctgcgggcg cgtccagaac acgttgacgg cgacgctgta
     2401 gatgcggccg aagcggcccg cgtccatcgc ctgcttgagc agctgcagcg tgcggttgcg
     2461 gcggttctgc ttgacgacga acaggcgcac gccggcctcg tcgcaggcgc gcaccatcgc
     2521 caggccgtcc tgccagcgcg tggccatggg cttctcggtc atgacgtcga tgccgcgcgc
     2581 ggccacttcc atcacctgct gcgagtgcag gccgctgggc gtcgtgacga ccacgcagtc
     2641 gggcgtctcg gcgtcgagca gctcgcgcag gctgcggtag cccgtcgcgc cggtgcgggc
     2701 gacggccgcg gcgagcttgg cggcgtccgt gtcgcacacc gccacgagct cggcgcgctc
     2761 ggcgtgcacc ttcatcgact ccatgtggtt ggcggcgatg cgcccgcagc cgaccagcgc
     2821 gaagcgcacc ttgcggtcgg tgacgggcgg cgcggaatgg gggcggaggc tgtgcgtgga
     2881 catgggacgt cgggcggggc gaaacgcgca attttaagtc agcgggacga cgccgccgcc
     2941 gcgccagccg gcgcggcggc ggacatccgg cataaaatca agggtttacc gcagggcccg
     3001 cctgcgttcc gctgccgtca tcggcgacgg cctacctacc cacgacatcc cacgcgcatg
     3061 gacacgacca cgccctccgc ctcccccgct cttccgctcg acgagaacaa gctcgtcgcg
     3121 gagcggcgcg agaaactggc cgccatccgc gcgcgcggcg gcgtcgcgtt cccgaacgac
     3181 ttcaagccga aggaccacgc ctgcgacctg cagcacaagt acggcaagtg caccaacgac
     3241 gagctcgagc cccgggccgt cgccgtcacg gtggccggcc gcatgatgct caagcgcacg
     3301 atgggcaagg cggcgttctg cacgctgcag gacgcgaccg gccgcatcca gctgcgcgtg
     3361 gcgatcgacc aggtcggcga ggagctgtac ggccagttca agcactggga tctgggcgac
     3421 atcctcggcg ccgagggcac gctgttcgtc acgaagacgg gcgagctgac catcctggtg
     3481 aagacgctgc gcctgctgac caagtcgctg cgcccgctgc ccgacaagtt ccacggcatg
     3541 accgaccagg agcagaaata ccgccagcgg tatgtcgacc tgatcaccga cgaggacgcc
     3601 cgcaagcgct tcatcgcgcg cagcaaggcg ctcggctcga tccgcggcta catgctggag
     3661 cacggcttcc tggaagtcga gacgccgatg ctgcacccga tccccggcgg tgccaacgcc
     3721 aagcccttca cgacgcacca caacgcgctc gaccaggaga tgttcctgcg catcgcgccc
     3781 gagctctacc tgaagcgcct ggtcgtcggc ggcttcgagc gcgtgttcga gatcaaccgc
     3841 aacttccgca acgaaggcat ctcggtccgg cacaacccgg agttcacgat gatggagttc
     3901 tacgcggcgt actggaacca ccacgacgtg atggacttca ccgaggccgt gctgcgccac
     3961 gccgcgcgcg cggccacggg ctcggcctcg ctgacctacg cgggcaagga agtgcacctc
     4021 gacaagccgt tcgagcgcct gtcggtgcgc gactcgctgg tccgctacgc cggcctggcc
     4081 gaggagcaga cccgcgacgc cgcagtgctg cgcgagcgca tcctggccgc cggcgaggag
     4141 gcccccgcac actggacgat cgccgagctg cagttcggcc tgttcgaggc ggtggtcgag
     4201 gcccagctgt ggcagccgac gttcatcatc gactacccgg tcgaggtgag cccgctcgcc
     4261 cgcgcgtccg acgccgaccc gacgatcacg gagcgcttcg agctgttcat caccggccgc
     4321 gaatacgcca acgggttctc cgagctcaac gacgccgagg accaggccgc gcgcttccag
     4381 gcccaggtcg ccaacaagga cgccggcgac gaggaggcga tgtactacga cgccgacttc
     4441 atccgcgcgc tcgagtacgg catgcccccg accggcggct gcggcatcgg catcgaccgc
     4501 ctgatgatgc tgctgaccga cagtccgacc atccgcgacg tgatcctgtt cccggcgctt
     4561 cggcgcgagg cctgagcccc cgcttttcgt tcgagggttt ttcgcgccgc agcatttgac
     4621 cgcggcgaag cgacgcatta ccgtggtcgc tcccttcccg cccggaggac tgccatgacc
     4681 gcgcgtctgc agcatctcga cgagatcgag caatgcatct ggagcgaact cgaacgttgc
     4741 gtggccccgc gcccgccccg agaggcggac gaaggccggg agccgcacga cctgcgggtg
     4801 gccgcgctgg agaccgagga cgcgcccggg gacggggact cgcccggcgt cgtgctgcgc
     4861 gaggtggacg cgccggagcg ccggctcgtc ttctacaccg acgcacgcgc gcccgaggtg
     4921 cggcgcctcg agtcgcatcc cgagggcacg ctgaccttcc gcagccgccg gctcggctgg
     4981 cagctgcgca tgcgggtgcg gctggccgtg gagcgcgacg gcctggccgt gtcgtcgcgc
     5041 tgggcccgcc tgcagacgcg cgcgggcgcg gacgactacc gcg
//
LOCUS       DASVLN010000615         2631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_246596, whole genome shotgun sequence.
ACCESSION   DASVLN010000615 DASVLN010000000
VERSION     DASVLN010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246596"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VF453_14770"
     CDS             <1..712
                     /locus_tag="VF453_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688975.1"
                     /translation="LSGPARDGAVLDQVRAGNVPEHLRHAKAVTLTAAGRPGSVTVCV
                     LPDYLAVGSDDDYVLVPLGLRAALALAAELGFELPTTRVVDAVYRQAAVHLDPRPLPA
                     GDEMRSTAYLLRHNAIVQAQRASQAGALDDLEAGSKKDLVLTRRLWEMPGRVAIYGWH
                     RLDGTPIQPLSLVHGARYADYSHGVRLVWSTAWLDGRPRRLEELLSRPQTAGWLSDEG
                     AIPLLERREELVAAADPP"
     gene            complement(721..1737)
                     /locus_tag="VF453_14775"
     CDS             complement(721..1737)
                     /locus_tag="VF453_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688976.1"
                     /translation="MRIRLAVRGLLIGALCLAVPRLAADAAPRALAASNEAIFEDLRD
                     IQGLDERQLAAIRAIFARSPFIGQGNPAVTVHPSTPAQCREARARAGLADDDPRFERV
                     CGAKHMAPLYDPARERPEDAKACIDQYEFPDVPCDYPVVWVRAREAAEICQAEGRRLC
                     DAHEWEGACEGRLAPPDYRFDLARGRPDEAAVDAMRSAHNRAESAHRTWPYGPAERSG
                     VCGTGGRKTPSCNGGSWAGCGSNTYPAGAFPGCRSALDVFDLEGNAAEHMNLPLDPTQ
                     MAGAGSRTLGVTEMKGSWFVFDTYHAHEDWCRWRAPFWHGSRVMDPRSHANYHLGFRC
                     CKSL"
     gene            complement(1734..>2631)
                     /locus_tag="VF453_14780"
     CDS             complement(1734..>2631)
                     /locus_tag="VF453_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688977.1"
                     /translation="WHADPVEADALDYFVAFDLARFELGFAIGTDHPRLGWSERAPQA
                     MRDAASPGPDGIDSAAPLVRTGLVDPADAARLVATFAGGFKREHGAFRYGRLATVNHA
                     SHYGFVEQGVVFSTLQPGLSTLYATADGRIDLHTWPGAAAPAASTLRFARQNGVPLVE
                     SDGLGRARLGALVGQWGAGNWSGSVDEQQRTLRSGACIVEDGRHRFLIYGWFSTATPE
                     TMARAFLALGCRDAMHLDMNALEHTYLALYVPEHGRLGVQHLADGMAVLDGSVGGALA
                     PRFVGAADDRDFFYVLEKAPTR"
BASE COUNT          328 a          947 c         1009 g          347 t
ORIGIN      
        1 actgagcggc ccggcgcgcg acggcgctgt tctcgaccag gttcgcgcgg gcaacgtccc
       61 ggagcacctg cggcacgcga aggccgtcac gctcaccgct gcgggccgac ccggcagcgt
      121 gacggtgtgc gtgttgcccg actacctggc cgtcggcagc gacgacgact acgtgctcgt
      181 gccgctgggc ctgcgcgccg cgctggcgct ggccgcggag ctcggcttcg agctgccgac
      241 cacccgcgtg gtggacgcgg tgtaccggca ggcggcggtc catctcgacc cgcgcccgct
      301 gccggccggc gacgagatgc gctccacggc ctacctgctg cgccacaacg ccatcgtgca
      361 ggcccagcgc gcctcgcagg ccggcgcgct ggacgacctg gaggcaggct ccaagaagga
      421 cctggtgctc acgcgccgcc tgtgggagat gcccgggcga gtggcgatct acggatggca
      481 ccggctcgac ggcacgccca tccagccgct cagcctggtg cacggcgcgc gctacgcgga
      541 ctacagccac ggcgtgcgcc tcgtgtggtc gaccgcgtgg ctggacggcc ggccgcggcg
      601 cctcgaggag ctgctgtcgc gcccgcagac cgccggctgg ctcagcgacg agggcgcgat
      661 cccgctgctg gagcggcgcg aggaactcgt cgccgccgcg gatcctccct gagccggcgt
      721 tcacaggctc ttgcagcagc ggaagccgag gtggtagttc gcgtggctgc gcggatccat
      781 cacccggctg ccgtgccaga acggcgcgcg ccagcggcac cagtcctcgt gcgcgtggta
      841 ggtgtcgaac acgaaccagc tgcccttcat ctcggtgacg ccgagcgtcc ggctgccggc
      901 gccggccatc tgcgtgggat cgagcggcag gttcatgtgc tccgccgcgt tgccttccag
      961 gtcgaagacg tcgagcgcgc tgcggcatcc ggggaacgcg ccggccggat aggtgttgga
     1021 cccgcatccc gcccagctgc ccccgttgca gctcggggtc ttgcggccgc cggtgccgca
     1081 gacgccgctg cgctcggcgg ggccgtaggg ccaggtgcgg tgcgccgatt cggcgcggtt
     1141 gtgggcgctg cgcatcgcgt cgaccgcggc ttcgtcgggg cgtccgcggg cgaggtcgaa
     1201 gcggtagtcc ggcggcgcga gccggccctc gcaggcgcct tcccactcgt gcgcgtcgca
     1261 caggcggcgt ccttcggcct ggcagatctc cgcggcttcg cgggcgcgga cccagaccac
     1321 cgggtagtcg cagggcacgt cggggaactc gtactggtcg atgcaggcct tggcgtcctc
     1381 cggccgttcg cgggcggggt cgtagagcgg cgccatgtgc ttcgcgccgc acacgcgctc
     1441 gaagcgcggg tcgtcgtccg ccagtccggc acgggcgcgc gcctcgcggc actgcgccgg
     1501 cgtcgacgga tgcaccgtca ccgccgggtt gccctggccg atgaagggcg agcgggcgaa
     1561 gatcgcgcgg atcgcggcga gctggcgctc gtccagcccc tggatgtcgc gcaagtcctc
     1621 gaagatcgcc tcgttgctcg cggcgagcgc ccgcggcgcc gcgtcggcgg cgagcctggg
     1681 caccgccagg cacagcgcgc cgatcagcag gccgcggacc gcgagccgga tcctcatcgc
     1741 gtcggcgcct tctcgaggac gtagaagaag tcgcggtcgt ccgcggcgcc cacgaagcgc
     1801 ggcgcgaggg cgccgccgac gctgccgtcg aggacggcca tcccgtccgc caggtgctgg
     1861 acgccgagcc gtccgtgctc ggggacgtag agcgcgaggt aggtgtgctc gagcgcgttc
     1921 atgtcgaggt gcatggcgtc gcggcagccc agggcgagga acgcgcgcgc catggtttcg
     1981 ggcgtggcgg tggagaacca gccgtagatc aggaagcggt gccggccgtc ctcgacgatg
     2041 caggcgcccg agcgcagcgt gcgctgctgc tcgtcgaccg atcccgacca gttgcccgcg
     2101 ccccactggc ccaccagcgc gcccaggcgg gcccgaccga ggccgtcgct ctcgaccagc
     2161 gggacgccgt tctggcgggc gaagcgcagg gtcgaggccg cgggcgcggc cgcgccgggc
     2221 caggtgtgca ggtcgatccg gccgtcggcc gtcgcgtaca gcgtggacag cccgggctgc
     2281 agggtgctga acacgacgcc ctgttccacg aagccgtagt ggctcgcgtg gttcacggtg
     2341 gccagccggc cgtagcggaa cgcgccgtgc tcgcgcttga agccgcccgc gaaggtggcg
     2401 accaggcgcg cggcgtctgc cgggtccacg aggccggtgc gcaccagcgg ggcggccgag
     2461 tcgatgccgt cggggcccgg gctggccgcg tcgcgcatcg cctgcggagc ccgctccgac
     2521 cagcccaggc gcggatggtc ggtgccgatc gcgaagccga gctcgaagcg cgccaggtcg
     2581 aaggcgacga agtagtccag cgcgtcggcc tcgaccggat cggcgtgcca g
//
LOCUS       DASVLN010000616         6701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_407815, whole genome shotgun sequence.
ACCESSION   DASVLN010000616 DASVLN010000000
VERSION     DASVLN010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6701
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407815"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VF453_14785"
     CDS             complement(<1..214)
                     /locus_tag="VF453_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX7688978.1"
                     /translation="MSASPLARLARLGLAAVLATAGAHAGAAIFKWRDAQGTTHYGEH
                     PPAGVKATPVDVDDPRPASERAAAQRL"
     gene            complement(321..1970)
                     /gene="pgm"
                     /locus_tag="VF453_14790"
     CDS             complement(321..1970)
                     /gene="pgm"
                     /locus_tag="VF453_14790"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HEX7688979.1"
                     /translation="MAQQLSPLAGKPAPRSALVDIPKLVSAYYTTLPDPVEPSQRVAF
                     GTSGHRGNAFDGSFNERHVLAITQAICDHRAAKGIDGPLFIGIDTHALSQPALASALE
                     VLAANGVTTMLSRDDEPVPTPAISHAIIVHNRGRTSGLADGIVVTPSHNPPDNGGFKY
                     NPPHGGPAAEDVTRGVQDAANRYLERNLEGVKRTTLARALAAPTTQRHDYLGRYVADL
                     GAAIDLDAIRDAKLHLGVDPLGGAGVHYWGRIAERYGLDLTVVSDVVDPTFGFMTLDW
                     DGRIRMDPSSPYAMQRLVDLKTRFDVAFACDTDHDRHGIVTKTSGLMLPNNYLAVAID
                     YLFRHRPQWGAQAAIGKTAVSSRLIDRVAQRLGRRMVEVPVGFKWFAAGLADGSLGFG
                     GEESAGAAFLRRDGASWTTDKDGITMALLSAEITARTGRDPGALYDEMTRELGRPVSD
                     RVEAPADAAQKKRLAALQPDAITTKEIAGEPITEVTATAPGNGAPIGGIRVGSANGWF
                     AARPSGTEEIYKIYAESFVGEAHLKRLLAEAQALVDGAIAG"
     gene            complement(2016..3434)
                     /gene="zwf"
                     /locus_tag="VF453_14795"
     CDS             complement(2016..3434)
                     /gene="zwf"
                     /locus_tag="VF453_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7688980.1"
                     /translation="MSDAARSDALVLFGFTGDLARKKIFPALYAMARRGELVPRIVGV
                     SGSSLSADEARERIRQSLEEEGGIDDPAAFERLTGAVRYVGGHYEDAATFERLKQALE
                     GARRPAHYLAVPPEMFEVVIAALGRTGLAKDARLIVEKPFGRDLASARRLDAAARKVF
                     PEEAIFRIDHYLGKEAIMNLLYFRFANAILDPLWCAEHIASVQVTMAEEFGVGTRGRF
                     YESVGALRDVVENHLFQVVALLAMEPPASTAFADLQAAKAALFKSMVPLAPDDLVRGQ
                     YAGYRDEDDVAAGSDVETYVALRMRIDSPRWRGVPFYLRAGKKLPKTTAEVVVQFKRP
                     ARALFADASAPGARPNWLRFQLQPYPSIALAARFKHVGKAFTGEQRELLACDELRGEE
                     APYDRLLGDAMAGDASLFTRADAVEAAWAVVDPVLADRPPVVAYQPGSWGPAQADALV
                     APDGGWIAPEPVAACATAASPS"
     gene            complement(3444..4457)
                     /gene="gnd"
                     /locus_tag="VF453_14800"
     CDS             complement(3444..4457)
                     /gene="gnd"
                     /locus_tag="VF453_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX7688981.1"
                     /translation="MQLGMIGLGRMGAAMARRLMAKGHACVVQDPNADAVARMVADGA
                     TGAASLQELVQKMERPRAVWLMVPAGIVDGVLAQLVPLLEKDDVVIDGGNSYYRDDIR
                     RAKELAARGLHYVDCGTSGGIAGQDRGYCLMVGAEDAVFARLAPIFEALAPGVGTQAR
                     TPGRTGDLSDAERGFLHCGPNGAGHFVMMVHNGIEYGMMAAFAEGFNILRHADIGKRP
                     QAHDAETTPLREPEFYQYEFDIPQIAEVWRRGSVVGSWLLDLTAKALVADPELKNFAG
                     RVSDSGEGRWTLQAGIDEGVPAHVLSAAVYDRFSSRGEATFANQILSAMRAEFGGHAE
                     KKG"
     gene            4657..5688
                     /locus_tag="VF453_14805"
     CDS             4657..5688
                     /locus_tag="VF453_14805"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX7688982.1"
                     /translation="MTLQALIDTATALLADHKGLLAMDESVPTCDKRFAALGIPQTAA
                     TRRAYRDLIVTAPGLAESIGGAILHDETIRGATLDGRPFVQALRDAGIVPGIKVDAGT
                     KPLALHPGETVTEGLDGLRERLAEYVSLGARFAKWRAVVAIGEGRPTHGAIAANAHAL
                     ARYAALCQEAGLVPIVEPEVLMDDDHTLARGAEVTERTLQVVFEALQGQGVKPEGMLL
                     KPNMVVAGLKCAAQPGADEVADATVAVLRRAVPAAVAGIAFLSGGQSPQDATARLNAM
                     NRRYAGGGLPWPVAFSYARAIQDPAMKAWAGDDANVTEAQRRLVERARLNRAARRGEY
                     QPSMEPGGG"
     gene            complement(5701..>6701)
                     /locus_tag="VF453_14810"
     CDS             complement(5701..>6701)
                     /locus_tag="VF453_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7688983.1"
                     /translation="RPADTPARSAVWAALAGNVLVAATKAGAAAFTGSAAMLSEAVHS
                     FVDTGNELLLLYGMRRAKKRADPDHPAGYGRELYFWSFVVALLVFALGAGVSIVQGIR
                     HVLQPADIESPRINELVLALSFLFEGGSWVVSWRQFRREKGGLGWYEALRRSKDPPLF
                     MVLMEDTAALVGIALAAIGTELAARAGLAWADGVASVLIGLVLVGVSTLLARESKSLL
                     IGERADAALTRSLLEIANAACGRSRANGALTIQMAPDQIMAAISFEFDDALRAPEIEA
                     MVADIECRIRAAHPEVTSLFVKPQDPDRFERGVRHRFGDEVAQDLLGGDAPPHVAS"
BASE COUNT          897 a         2399 c         2501 g          904 t
ORIGIN      
        1 ccaggcgctg cgcggcggcg cgctcggacg ccgggcgagg gtcgtccacg tcgaccggcg
       61 tggccttcac gcccgcgggc gggtgctcgc cgtagtgcgt ggtgccctgc gcgtcgcgcc
      121 acttgaagat cgccgcgccc gcatgcgcgc cggccgtcgc gaggacggcg gcgagaccca
      181 ggcgggcgag gcgggcaagc ggggaggcgc tcatgagacg tgctccgccc tgccgacttc
      241 ggtgcgagcc cgtttccgag cggccgggca gcggatcatg cgcgattctg cgcccgccgc
      301 cggggcgggt cgagaggcct tcagcccgcg atcgcgccgt cgaccagcgc ctgcgcttcc
      361 gcgagcagcc gcttcaggtg cgcctcgccg acgaagcttt cggcgtagat cttgtagatc
      421 tcctcggtgc cggaaggccg cgccgcgaac cagccgttgg ccgacccgac gcggatgccg
      481 ccgatcgggg cgccgttgcc gggcgcggtc gcggtgacct cggtgatggg ctcgcccgcg
      541 atctccttcg tcgtgatcgc atccggctgc agcgcggcca ggcgcttctt ctgcgcggca
      601 tcggcgggcg cctcgacgcg gtcggacacc gggcgtccga gctcgcgcgt catctcgtcg
      661 tacaacgctc ccgggtcgcg gccggtgcgg gccgtgatct cggccgacag cagcgccatc
      721 gtgatgccgt ccttgtcggt cgtccacgac gcgccgtcgc ggcgcaggaa cgccgcgccc
      781 gcgctctcct cgccgccgaa gccgagcgat ccgtcggcga ggccggcggc gaaccacttg
      841 aagccgacgg gcacctcgac catccgccgc ccgagccgct gcgcgacgcg gtcgatcagg
      901 cgcgaggaca ccgccgtctt gccgatggcc gcctgcgcgc cccactgcgg gcggtggcgg
      961 aacaggtagt cgatcgcgac cgcgaggtag ttgttcggca gcatgagccc gctcgtcttc
     1021 gtgacgatgc cgtggcggtc gtggtcggtg tcgcacgcga aggcgacgtc gaagcgcgtc
     1081 ttcaggtcga ccaggcgctg catcgcatac ggcgaggacg ggtccatgcg gatccggccg
     1141 tcccagtcca gcgtcatgaa gccgaaggtc ggatcgacga cgtcgctgac gacggtgagg
     1201 tcgagcccgt agcgctcggc gatgcgaccc cagtagtgca cgcccgcgcc gcccagcggg
     1261 tcgacgccca ggtgcagctt cgcgtcgcgg atcgcgtcga ggtcgatggc cgcgccgagg
     1321 tcggcgacat accggcccag gtagtcgtgg cgctgcgtgg tcggggcggc cagggcgcgg
     1381 gcgagcgtcg tgcgcttgac accctcgagg ttgcgctcga ggtaccggtt cgccgcgtcc
     1441 tgcacgcccc tggtgacgtc ctcggcggcc ggtccgccgt gcggcgggtt gtacttgaag
     1501 ccgccgttgt cgggcgggtt gtgcgagggc gtgacgacga tgccgtcggc caggccgctc
     1561 gtgcgcccgc ggttgtggac gatgatcgcg tgcgagatcg ccggcgtggg cacgggttcg
     1621 tcgtcccgcg acagcatcgt cgtcacgccg ttggcggcca gcacctccag cgcgctcgcg
     1681 agcgccggct gcgacagcgc atgcgtgtcg atgccgatga acagcgggcc gtcgatgccc
     1741 ttggcggcgc ggtggtcgca gatcgcctgc gtgatcgcga gcacgtggcg ctcgttgaac
     1801 gagccgtcga acgcgttgcc gcggtggccc gaggtgccga acgcgacgcg ctgcgagggc
     1861 tcgacggggt cgggcagcgt cgtgtagtac gcgctgacca gcttcgggat gtcgacgagc
     1921 gcggagcgcg gggccggctt gccggcgagc ggggagagct gctgggccat gtcgtggtgc
     1981 gtcggtgttg gcgtgcgggg gcgatcttcg ccgattcagg aaggcgaggc ggcggtcgcg
     2041 caggcagcga ccggctcggg cgcgatccag ccgccgtcgg gcgccacgag cgcatccgcc
     2101 tgcgccgggc cccagctgcc gggttggtag gcgacgacgg gcggacgatc ggcgagcacg
     2161 gggtcgacga cggcccacgc ggcctcgacg gcgtccgcgc gcgtgaacag ggacgcgtcg
     2221 cccgccatcg cgtcgccgag gagccggtcg tagggcgcct cctcgccgcg cagctcgtcg
     2281 caggcgagca gttcgcgctg ctcgcccgtg aaggccttgc cgacgtgctt gaagcgcgcc
     2341 gccagggcga tcgacgggta gggctgcaac tggaagcgca gccagttcgg ccgcgcgccg
     2401 ggcgcggacg cgtccgcgaa cagcgcccgc gcgggccgct tgaactgcac cacgacttcg
     2461 gcggtcgtct tcggcagctt cttgccggcg cgcaggtaga acggcacgcc gcgccagcgc
     2521 ggggagtcga tgcgcatgcg cagcgcgaca tacgtctcga cgtcggagcc ggccgcgacg
     2581 tcgtcctcgt cgcgatagcc cgcgtactgg ccgcgcacga gatcgtcggg ggcgagcggc
     2641 accatggact tgaacagcgc cgccttggcc gcctgcaggt cggcgaaggc ggtggaggcc
     2701 ggcggctcca tcgccagcag cgcgacgacc tggaacaggt ggttctcgac gacgtcgcgc
     2761 agcgcgccca cgctctcgta gaaccggccg cgcgtgccga cgccgaactc ctcggccatc
     2821 gtcacctgca cgctcgcgat gtgctcggcg caccagagcg gatcgaggat cgcgttggcg
     2881 aagcggaagt acaggaggtt catgatcgcc tccttgccga ggtagtggtc gatgcggaag
     2941 atcgcctcct cgggaaacac cttgcgcgcc gcggcgtcga gcctgcgcgc cgacgcgagg
     3001 tcgcggccga agggcttctc gacgatcagc cgggcgtcct tcgcgagccc ggtgcggccg
     3061 agcgccgcga tcaccacctc gaacatctcg ggcggcaccg cgaggtagtg cgccggccgg
     3121 cgcgcgccct cgagtgcctg cttcaggcgc tcgaaggtcg cggcgtcctc gtagtggccg
     3181 ccgacgtagc gcacggcgcc ggtgagccgc tcgaacgcgg ccgggtcgtc gatgccgccc
     3241 tcctcctcaa ggctctggcg gatgcgttcg cgtgcctcgt cggcgctcag cgacgagccc
     3301 gagacgccga cgatgcgcgg cacgagctcg ccgcgccgcg ccatcgcgta cagcgcgggg
     3361 aagatcttct tgcgcgcgag gtcgccggtg aagccgaaga ggacgagggc gtcggagcgt
     3421 gcggcgtcgg acatcgggac gggctagcct ttcttctccg catggccgcc gaactcggcg
     3481 cgcatcgcgg acaggatctg gttggcgaag gtcgcctccc cgcgcgagga gaagcggtcg
     3541 tagacggcgg cgctgagcac gtgcgcgggc acgccttcgt cgatgccggc ctgcagcgtc
     3601 cagcggccct cgccggagtc cgagacgcgg ccggcgaagt tcttcagctc cggatcggcg
     3661 accagcgcct tggcggtcag gtccagcagc cacgagccga cgacgctgcc gcggcgccag
     3721 acctccgcga tctgcgggat gtcgaactcg tactggtaga actcgggctc gcgcagcggc
     3781 gtggtctcgg catcgtgcgc ctgcgggcgc ttgccgatgt ccgcgtggcg caggatgttg
     3841 aagccctcgg cgaaggcggc catcatcccg tactcgatgc cgttgtggac catcatcacg
     3901 aagtggccgg cgccgttcgg gccgcagtgc aggaagccgc gctccgcgtc cgagaggtcg
     3961 ccggtgcggc ccggcgtgcg ggcctgcgtg ccgacgcccg gggcgagcgc ctcgaagatc
     4021 ggcgcgaggc gcgcgaagac cgcgtcctcg gcgcccacca tcaggcagta gccgcgatcc
     4081 tggccggcga tgccgccgct ggtgccgcaa tcgacgtagt gcaggccgcg ggcggcgagc
     4141 tccttcgcgc ggcggatgtc gtcgcggtag taggagttgc cgccgtcgat gaccacgtcg
     4201 tccttctcga gcagcggcac gagctgcgcc agcacgccgt cgacgatgcc cgcgggcacc
     4261 atcagccaga ccgcgcgcgg gcgctccatc ttctgcacga gctcctgcag cgaggccgcg
     4321 ccggtcgcgc cgtcggcgac catcctggcg acggcgtcgg cgttggggtc ctgcaccacg
     4381 cacgcgtgtc ccttggccat caggcgacgc gccatcgccg cgcccatgcg gcccaatccg
     4441 atcattccca gttgcacggt ggtctttcga agtcgaagcg aagcgcgcat catcgcgcag
     4501 ggccgccggg gacgtcgacc gaagggccgg cgcccgcgtc gcggcggtgg caatccacat
     4561 tcccgtcgga cgccgccggc gtgcggcggt cgatcgacgc gggtgcgcgg attgccgctc
     4621 gcgcaccccg tttcccgtcg tccggaaagg cgcgccatga ccctgcaagc cctcatcgac
     4681 accgcgaccg cgctgctcgc cgaccacaag ggcctgctgg cgatggacga gagcgtgccc
     4741 acctgcgaca agcgcttcgc ggcgctgggc atcccgcaga ccgcggcgac gcggcgcgca
     4801 taccgcgacc tcatcgtgac ggcgcccggc ctggccgagt ccatcggcgg cgcgatcctc
     4861 cacgacgaga cgatccgcgg cgcgacgctg gacggccgcc cgttcgtgca ggcgctgcgc
     4921 gacgccggca tcgtcccggg catcaaggtc gacgcaggca cgaagccgct cgcgctgcat
     4981 ccgggcgaga ccgtgaccga aggcctggac ggcctgcgcg agcgcctggc cgagtacgtc
     5041 tcgctcggcg cgcgcttcgc gaagtggcgc gcggtcgtcg cgatcggcga gggcaggccc
     5101 acgcatggcg cgatcgccgc caacgcgcat gcgctcgcac gctacgccgc gctgtgccag
     5161 gaggcgggcc tcgtccccat cgtcgagccc gaggtgctga tggacgacga ccacacgctc
     5221 gcgcgcggcg ccgaggtcac cgagcgcacg ctgcaggtcg tcttcgaggc cctgcagggc
     5281 cagggcgtga agcccgaggg catgctgctc aagcccaaca tggtcgtggc gggcctgaag
     5341 tgcgccgcgc agccgggcgc cgacgaggtc gccgacgcca ccgtcgccgt gctcaggcgc
     5401 gcggtgccgg ccgcggtggc ggggatcgcc ttcctctccg gcggacagtc gccgcaggac
     5461 gcgaccgcgc ggctgaacgc gatgaaccgg cgctacgccg gcggcgggct gccgtggccg
     5521 gtcgcgttct cgtacgcgcg ggcgatccag gatccggcga tgaaagcctg ggcgggcgac
     5581 gacgccaacg tcaccgaggc gcagcgccgg ctcgtcgagc gcgcgcgcct caaccgcgcc
     5641 gcgcggcggg gcgagtacca gccgtcgatg gagcccggcg gcggctgaag cgcgggccgg
     5701 tcaggaagcc acgtgcggcg gcgcgtcgcc gcccaggagg tcctgcgcga cctcgtcgcc
     5761 gaagcggtgc cgcacgccgc gctcgaagcg gtcgggatcc tgcggcttga cgaacagcga
     5821 cgtgacctcg ggatgcgccg cgcggatgcg gcactcgatg tcggcgacca tcgcctcgat
     5881 ctcgggcgcg cgcagggcgt cgtcgaactc gaagctgatc gcggccatga tctggtcggg
     5941 cgccatctgg atcgtgagcg cgccgttggc ccgcgagcgc ccgcaggccg cgttggcgat
     6001 ctccagcagc gagcgcgtca gcgccgcgtc cgcgcgttcg ccgatcagca ggctcttgct
     6061 ctcgcgcgcg agcagcgtcg atacccccac caggaccagc ccgatcagca ccgaggccac
     6121 gccgtcggcc caggcgaggc ccgcgcgcgc ggcgagctcg gtgccgatcg cggcgagcgc
     6181 gatgcccacg agcgccgcgg tgtcctccat cagcaccatg aacagcggcg gatccttgct
     6241 ccggcgcagc gcctcgtacc agccgaggcc gcctttctcg cgccggaact ggcgccacga
     6301 cacgacccac gacccgccct cgaacaggaa cgacagcgcg agcacgagct cgttgatgcg
     6361 cgggctctcg atgtcggcgg gctgcagcac gtggcggatg ccctgcacga tggacacgcc
     6421 cgccccgagc gcgaacacga gcagggcgac gacgaagctc cagaagtaca gctcgcggcc
     6481 atagccggcc ggatggtcgg ggtcggcgcg cttcttcgcc cggcgcatgc cgtacagcaa
     6541 cagcagctcg ttgccggtgt cgacgaagga gtgcaccgcc tcgctcagca tcgccgcgct
     6601 gccggtgaag gccgcggcac cggccttggt cgcggccacg agcacgttgc ccgcgagcgc
     6661 cgcccacacg gcgctgcgcg cgggcgtgtc cgcgggccgc g
//
LOCUS       DASVLN010000617         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_141757, whole genome shotgun sequence.
ACCESSION   DASVLN010000617 DASVLN010000000
VERSION     DASVLN010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141757"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VF453_14815"
     CDS             <1..364
                     /locus_tag="VF453_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7688984.1"
                     /translation="PMRETRGAAVALAQLDPRAGALAFAGAGNIAGRMISGVDDRSLM
                     SQHGTLGLQIRRLQDVGYAWPEHAIVVLHSDGLTTRWRLDDAPGLLQCDPAVIAGWLL
                     RDHVRGHDDATVVVLKRE"
     gene            367..1251
                     /locus_tag="VF453_14820"
     CDS             367..1251
                     /locus_tag="VF453_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7688985.1"
                     /translation="MDEPDRVAALQAELDEQRALFAALQDELEETNRGVVALYSELDT
                     QAQQLQRATELKSRFLAYMSHEFRTPISAIRSLARLLLDEVDGPLNHEQARQVRFIDT
                     TVGEFSEMVDDLLDLAKVEAGRVDISPAWFEMVDLFSTLRGMFRPVLTNPEVSLVFEE
                     PQQASRLYTDDKKLGQILRNFISNALKFTARGEVRISAVENADGTITFAVADTGIGID
                     PQFHPAIFQDFAQIDSPVQKRLRGTGLGLSLSKRLAQLLGGSVGLESALGRGSTFSVT
                     IPLRLAEPPADAGPGARP"
     gene            1248..3230
                     /locus_tag="VF453_14825"
     CDS             1248..3230
                     /locus_tag="VF453_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7688986.1"
                     /translation="MTEQTPEFRPIDRSQHELLVVDDNPASRYATVRQLRAAGFRTRE
                     AATGAEGLALADDGISAIVLDIHLPDIDGLELCARLRSRPSNSRMPVLHLTAAYVTDD
                     DKVRGLDNGADAYLTRPVEPAVLVATVQALVRTRVAEEAMRRSEQKFRAIYAQSLSGI
                     ALLDDAGVLIDANPAMESLLGRPHADLVSHALVEFAPEAWVQATAEFVAHQGAPVTGR
                     ELPMRRPDGSLAYLEWTLSPHIEPGVTMATATDVEDRVRLAHQRQQLLDRERIARGEA
                     EQVNRMKDDFIAVLSHELRTPLNAIMGWTHVLQQRGGTPEALKGLAAIERNGRVQARI
                     ISDLLDMSRLNLGKLPLNFATLDPAEELQAAVAALRPATDSSGVSIDLDLQPSYRPIR
                     ADSQRLQQVVWNLLSNAVKFSPQGGRVAVAMSQEHAGLRIRVSDSGQGIAPEFLPFVF
                     DRFAQSDAASNRQRGGLGLGLSIVKQLVEAHGGTVSASSAGVGRGATFEVFLPAAQEP
                     RDEPEWAETGRGGLDPLHKEALLDGVRLLVVDDDAEAGAMLQLILGDHGAQVRVARDV
                     ETALRMADMEAPDLVVSDIGMPGRDGYDLVRELRRREALVPGRTARLPAIALTSFTRD
                     QDRQQALAAGFDLHSPKPVRPLALVQQIRQLLDRRR"
BASE COUNT          469 a         1232 c         1201 g          452 t
ORIGIN      
        1 gccgatgcgc gagacgcgcg gcgccgccgt ggcgctggcc cagctcgacc cgcgcgccgg
       61 cgcgctcgcc ttcgccggcg ccggcaacat cgccgggcgc atgatctcgg gtgtagacga
      121 ccgttcgctg atgtcgcaac atggcaccct gggcctgcag atccggcgcc tgcaggacgt
      181 cggctacgcc tggcccgagc acgccatcgt cgtgctgcat tcggacggac tgaccacgcg
      241 ctggcgcctc gacgacgcgc ccggcctgct gcagtgcgat ccggcggtga tcgccggctg
      301 gctgctgcgc gaccatgtgc gcggccacga cgacgcgacg gtggtcgtgc tgaagcggga
      361 gtgaccatgg acgaaccgga ccgcgtcgcc gcgctgcagg cggagctcga cgagcagcgc
      421 gccctgttcg cggcgctgca ggacgagctc gaggagacca accgcggcgt cgtcgccctg
      481 tactccgagc tcgacaccca ggcccagcag ctccagcgcg ccaccgagct gaagagccgc
      541 ttcctcgcct acatgagcca cgagttccgc acgccgatct cggccatccg cagcctcgcg
      601 cgcttgctgc tggacgaggt cgacgggccg ctcaaccacg agcaggcgcg ccaggtgcga
      661 ttcatcgaca cgacggtggg cgagttctcc gagatggtcg acgacctgct ggatctcgcc
      721 aaggtcgagg cagggcgggt cgacatctcg cccgcgtggt tcgagatggt ggacctgttc
      781 tcgacgctgc gcggcatgtt ccgcccggtg ctgaccaacc cggaggtctc gctcgtcttc
      841 gaggagccgc agcaggcgtc gcgcctgtac acggacgaca agaagctcgg gcagatcctg
      901 cgcaacttca tctccaacgc gctgaagttc accgcgcgcg gcgaggtgcg gatctccgcg
      961 gtcgagaacg cggacggcac gatcaccttc gccgtcgccg acaccggcat cggcatcgac
     1021 ccgcagttcc atccggcgat cttccaggac ttcgcgcaga tcgactcgcc ggtgcagaag
     1081 cgcctgcgcg gcacggggct cgggctgtcc ctcagcaagc ggctcgcgca gctgctgggc
     1141 ggcagcgtcg gcctcgagag cgcgctcggg cgcggatcga ccttctccgt gaccatcccc
     1201 ctgcgcctgg ccgagccgcc ggccgacgca gggccgggag cccgtccatg accgagcaga
     1261 cgcccgagtt ccgtccgatc gaccgctcgc agcacgagct gctggtcgtc gacgacaacc
     1321 ccgccagccg ctacgccacg gtgcgccagc tgcgcgccgc gggctttcgc acccgcgaag
     1381 cggccaccgg cgccgagggc ctcgcgctcg ccgacgacgg catctcggcg atcgtgctgg
     1441 acatccacct gcccgacatc gacggcctcg agctgtgcgc tcggctgcgt tcgcgtccct
     1501 ccaactcgcg catgccggtc ctgcacctga cggccgccta cgtgaccgac gacgacaagg
     1561 tgcgcgggct ggacaacggc gccgacgcct acctgacgcg gcccgtggag ccggccgtcc
     1621 tcgtggcgac ggtgcaggcg ctcgtgcgca cgcgcgtggc cgaggaggcg atgcgccgca
     1681 gcgagcagaa gttccgcgcc atctacgcgc agtcgctttc cggcatcgcg ctgctggacg
     1741 atgccggggt gctgatcgac gccaatccgg cgatggagtc gctgctcggg cgtccgcacg
     1801 cggacctggt gagccatgcg ctcgtcgagt tcgcgccgga ggcatgggtg caggcgacgg
     1861 cggagttcgt cgcccaccag ggcgcgccgg tgacgggccg cgagctgccg atgcgccgcc
     1921 ccgacggctc gctcgcctac ctcgagtgga cgctgtcgcc ccacatcgag ccgggcgtga
     1981 cgatggccac ggcgaccgac gtcgaggatc gcgtgcgcct cgcgcaccag cgccagcagc
     2041 tgctggaccg cgagcgcatc gcgcgcggcg aggcggagca ggtcaaccgc atgaaggacg
     2101 acttcatcgc cgtgctgtcg cacgagctgc gcacgccgct gaacgcgatc atgggctgga
     2161 cgcacgtgct gcagcagcgc ggcggcacgc cggaggcgct caagggcctg gccgcgatcg
     2221 agcgcaacgg ccgcgtgcag gcgcgcatca tctccgacct gctggacatg tcgcgcctga
     2281 acctgggcaa gctgccgctg aacttcgcga cgctcgaccc cgccgaggag ctgcaggccg
     2341 cggtcgccgc gctgcgtccg gcgacggaca gcagcggcgt gtcgatcgac ctcgacctgc
     2401 agccgtcgta ccggccgatc cgcgcggatt cgcaacgcct gcagcaggtg gtctggaacc
     2461 tcctgagcaa cgcggtgaag ttctccccgc agggcggccg ggtcgcggtc gcgatgtcgc
     2521 aggagcatgc cggcctgcgc atccgcgtga gcgacagcgg ccagggcatc gcgcccgagt
     2581 tcctgccctt cgtgttcgac cgtttcgccc agagcgacgc ggcgagcaac cgccagcgcg
     2641 gcggcctcgg cctcgggctg tcgatcgtca agcaactcgt ggaggcgcat ggcgggacgg
     2701 tgtcggcgtc gagcgcgggg gtcgggcgcg gcgccacgtt cgaggtcttc ctgcccgcgg
     2761 cccaggagcc gcgcgacgag cccgagtggg cggagaccgg ccgcggcggg ctcgatccgc
     2821 tgcacaagga ggcgctgctg gacggcgtgc gcctcctggt ggtcgacgac gacgccgagg
     2881 cgggcgcgat gctgcagctg atcctcggcg accacggtgc gcaggtgcgc gtcgcgcgcg
     2941 acgtggagac ggcgctgcgg atggccgaca tggaggcgcc cgacctggtc gtgagcgaca
     3001 tcggcatgcc cgggcgcgac ggctacgacc tggtgcgcga gctgcgccgg cgcgaggcgc
     3061 tcgtgcccgg tcgcaccgcc cgcctgcccg cgatcgcgtt gacgtcgttc acgcgcgacc
     3121 aggatcgcca gcaggcgctg gccgcgggat tcgacctcca cagccccaag ccggtgcggc
     3181 ccctggcgct ggtgcagcag atccgccagt tgctcgaccg gcgtcggtga gaatccgcgg
     3241 atgcgcgccg cggcccgggc ccctcggccg gcgcgccctc gcatgatcga cgaccgtccc
     3301 tccgccgacg accgcaccga gcgcgcccgc gccttgctgg gcgcgttccg gcgc
//
LOCUS       DASVLN010000618         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_246650, whole genome shotgun sequence.
ACCESSION   DASVLN010000618 DASVLN010000000
VERSION     DASVLN010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246650"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /gene="pyrF"
                     /locus_tag="VF453_14830"
     CDS             complement(<1..801)
                     /gene="pyrF"
                     /locus_tag="VF453_14830"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEX7688987.1"
                     /translation="MRFTDTLRAAAARNDSCLCVGLDPEPAKFPGAWKGDAGKVFEFC
                     ARIVDATKDVVSAYKPQIAYFAAQRAEDQLEQLMAYIRRTAPGVPVILDAKRGDIGAT
                     AEQYAREAFVRYQADAVTLSPFMGYDSLEPYMRHDGKGLILLCRTSNPGGSDLQAQVL
                     SSGDHLYEHLARLAAGEWNKGGQLGLVVGATFPAELARVRQLAPTLPLLIPGIGAQGG
                     DIEATVRAGWREDGPIIVSSSRAILYAGAGEDFAAAARQVAVATRTALN"
     gene            complement(878..2251)
                     /gene="purB"
                     /locus_tag="VF453_14835"
     CDS             complement(878..2251)
                     /gene="purB"
                     /locus_tag="VF453_14835"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX7688988.1"
                     /translation="MAHSALTALSPLDGRYAPKLAALRPLLSEYGLMHRRVQVEVEWF
                     IALADAGFAEFPPLPESAKGLLRSLVVRFSEADAQAIKDIEKETNHDVKAVEYWLRQR
                     IAHVPELKEKCEFIHFACTSEDINNTSHALMLKAAREQVLLPALDGILTRLQAMAQRF
                     AAVPMLSRTHGQTASPTTVGKEIANVVARLAHARARVAEVVLLAKMNGAVGNYNAHLA
                     AYPETDWEAFSKKVVEERLGLKFNPYTIQIEPHDYMAELFDANVRVNTILVDWARDVW
                     GYISLGYFKQRTKPGEVGSSTMPHKVNPIDFENAEGNLGMANAMLTHMSQKLPISRWQ
                     RDLTDSTVLRNVGVALGYSILAYDSLTRGLDKLELNEAALAADLDGAWEVLAEPIQTV
                     MRRHGLPDPYDQLKKFTRGQPMTRELIQGFIAALDLPEGEKARLLAMTPASYVGAAAQ
                     LARDVKS"
BASE COUNT          359 a          916 c         1030 g          412 t
ORIGIN      
        1 gttcagagcg gtgcgcgtcg ccacggcgac ctggcgggcg gcggcggcga agtcctcgcc
       61 cgcgcccgcg tacaggatcg cgcgcgagga gctgacgatg atcgggccgt cctcgcgcca
      121 gccggcgcgc acggtggcct cgatgtcgcc gccctgcgcg ccgatgcccg ggatcagcag
      181 cggcagcgtc ggtgcgagct ggcgcacgcg cgcgagctcc gccgggaagg tcgcgccgac
      241 gaccagcccc agctgcccgc ccttgttcca ttcgccggcg gccaggcggg cgaggtgctc
      301 gtagaggtgg tcgcccgagc tcagcacctg cgcctgcagg tcggagccgc cggggttgga
      361 cgtgcggcac agcaggatca ggcccttgcc gtcgtggcgc atgtagggct cgagcgagtc
      421 gtagcccatg aaaggcgaca gcgtcaccgc gtcggcctgg tagcggacga aggcctcgcg
      481 ggcgtactgc tcggcggtcg cgccgatgtc gccgcgcttg gcgtccagga tcaccggcac
      541 gccgggcgcg gtgcggcgga tgtaggccat cagctgctcg agctggtcct cggcgcgctg
      601 ggcggcgaag taggcgatct gcggcttgta ggccgacacc acgtccttgg tcgcgtcgac
      661 gatgcgggcg cagaactcga acaccttgcc cgcgtcgccc ttccaggcgc ctgggaactt
      721 cgccggctcg gggtcgaggc ccacgcacag gcacgagtcg ttgcgggcgg cggcggcgcg
      781 cagggtgtcg gtgaatcgca tggcgggagg gttccttcgt cggggtcgtc tgggcggggg
      841 cggatgcgaa aacgcggccg gaggggccgc gtcgtcgtca ggacttgacg tccctcgcga
      901 gctgggccgc ggcgcccacg tagctggccg gcgtcatcgc cagcagccgc gccttctcgc
      961 cctcgggcag gtccagcgcc gcgatgaagc cctggatcag ctcgcgcgtc atcggctggc
     1021 cgcgcgtgaa cttcttgagc tggtcgtacg ggtcgggcag cccgtggcgg cgcatcacgg
     1081 tctggatcgg ctcggcgagc acctcccacg cgccgtcgag gtcggcggcc agcgcggcct
     1141 cgttgagctc gagcttgtcc aggccgcgcg tcaggctgtc gtaggcgagg atcgagtagc
     1201 ccagcgccac gcccacgttg cgcagcacgg tggaatcggt gaggtcgcgc tgccagcggc
     1261 tgatcggcag cttctgcgac atgtgcgtga gcatcgcgtt ggccatgccg aggttgccct
     1321 ccgcgttctc gaagtcgatc gggttgacct tgtgcggcat ggtcgacgag ccgacctcgc
     1381 ccggcttcgt cctctgcttg aagtagccga ggctgatgta gccccagacg tcgcgcgccc
     1441 agtcgacgag gatggtgttg acgcgcacgt tggcgtcgaa cagctcggcc atgtagtcgt
     1501 gcggctcgat ctggatcgtg tacgggttga acttcaggcc caggcgctcc tcgacgacct
     1561 tcttcgagaa cgcctcccag tcggtttcgg gataggccgc caggtgcgcg ttgtagttgc
     1621 cgacggcgcc gttcatcttc gcgagcagca ccacctcggc gacgcgcgcg cgggcgtgcg
     1681 cgaggcgcgc gacgacgttg gcgatctcct tgccgacggt cgtcgggctg gcggtctggc
     1741 cgtgcgtgcg cgagagcatg ggcaccgccg cgaagcgttg cgccatcgcc tgcaggcgcg
     1801 tgaggatgcc gtcgagcgcc ggcagcagca cctgctcgcg cgcggccttg agcatcagcg
     1861 cgtggctggt gttgttgatg tcctcgctgg tgcaggcgaa gtggatgaac tcgcacttct
     1921 ccttcagctc gggcacgtgg gcgatgcgct ggcgcagcca gtactcgacc gccttcacgt
     1981 cgtggttggt ctccttctcg atgtccttga tcgcctgcgc gtcggcctcg gagaagcgca
     2041 ccaccagcga gcgcagcagg cccttggcgg actcgggcag cggcgggaac tcggcgaagc
     2101 cggcatccgc cagcgcgatg aaccactcga cctcgacctg cacgcggcga tgcatcaggc
     2161 cgtattcgga cagcagcggg cgcagcgcgg ccagcttggg ggcgtagcgg ccgtcgagcg
     2221 gcgacagggc ggtgagggcg gaatgggcca tgaggacttt cggcaaaggt gcgcccgcgg
     2281 acggcgggcg cggcgcgggg cggcgacgcg tgggtgcgtc gcgacccggg agcacgggcg
     2341 ggtggcgagg gccgcggcgc agggcgggcg ccggcgccgc gccccgcaaa accaagggat
     2401 tttacgcatc cgcggccggt ccaggtagcc gggcagggag ggttcggaac aagccttccg
     2461 gcccgcggcg gacaaattcc gcgtcccgga gcgcaagctc acgggccgcc ggccgcgcca
     2521 gggccaataa tcgcgcgtcc gccgcgcacc gcggcatgtc aacgaggacg acccgcaccg
     2581 cacgggtcgc gacagggaga ttccatgctt cgaggcctcc ggcgtctgaa cgacgcgctc
     2641 ggcgccgtcc tgcgcgcgct cgcccacggc ctggccgtcc tcggccggct gctggcgccg
     2701 gtcgggcgcg ccgtgct
//
LOCUS       DASVLN010000619         9514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_246908, whole genome shotgun sequence.
ACCESSION   DASVLN010000619 DASVLN010000000
VERSION     DASVLN010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246908"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VF453_14840"
     CDS             <1..271
                     /locus_tag="VF453_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2894 domain-containing protein"
                     /protein_id="HEX7688989.1"
                     /translation="PGTPPVSPASRPAAADAESLQYFRRTWSRLAAEQHLAQSRSSLP
                     GNAGPLHSHTLVHRSLALMRELSPGYFEHFMAHVDALAWLEAADE"
     gene            complement(348..596)
                     /locus_tag="VF453_14845"
     CDS             complement(348..596)
                     /locus_tag="VF453_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7688990.1"
                     /translation="MFLKELYLRRDARGRGVGAALMRWVARHAEALGCVRIDWTVEAG
                     NAAAQAFYGALGAGRVAQRWHMRLDRDGLERLAETDDA"
     gene            complement(597..869)
                     /locus_tag="VF453_14850"
     CDS             complement(597..869)
                     /locus_tag="VF453_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688991.1"
                     /translation="MRIDCLAPSDTGRPEAVDRFADLLHDLGAHYQGEAAPSREAVRS
                     HLVERLMMPDSPLRFALAVADDGRALGFAAVLSWHSLVDCSAERSR"
     gene            1064..3913
                     /locus_tag="VF453_14855"
     CDS             1064..3913
                     /locus_tag="VF453_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7688992.1"
                     /translation="MSNAVPRSLPRLLRFLVPQTLASRASLAISAVFLVGVAAMAVVS
                     LSTFESQLAAVLRGEQDLVVGRIAEDVDQRIELLQEALRDSAAHITEADLADYASARA
                     ALERNDGLAAVFDRSVFLFSARGALVAERPYRTDRLGQDATWRPYIRRTMDTGQPQIS
                     EPFKTNMGDGNFVMVLTWPVRGHDGRLIGMITGSLGLTRPDLLGSVAKTRFGRTGYLS
                     ITTADGRLLIDPEPSRLSSALYAPGANEPFERALKGFEGTASTVDENGRPSFVTYRRV
                     KSAGWLVAAVYPRDEAYEYTVRFVRNFLLALAAAALAVLWAIWTLTRYLTRPLDALSR
                     HIADYTATEGRIEPLAGVEGSGEVRVLTTAFNALTRRLNEREESMVSALRQYQVITES
                     STDLITRHVRNGTIAFASPASRRVLGVEEAALRGRHVGDLAHPEDLQRLLAAFDAAWT
                     SDGATVVAWRARTPADEHVWLESALRPLPGSGKGGEILCLSRNIDERKRLEEKLHREA
                     RLDRLTQLPNRLLLDERLPAAMSRCHREGSLLAVLLIDLDRFKDINDTLGHRRGDDLL
                     AAVAARFASVARTGETVARWGGDEFVVVLPGLSHPDVARLVAERFLKALKEPFEFGGQ
                     ALQVSASIGIAVTGDDSVATETLLANADVAMYRAKRRGGNASVTWASEMSEGAQDRLS
                     MEGALFHAVERDELRLHYQPLVSARNGRIVGVEALLRWEHPEFGLVSPGVFIPIAERI
                     GVIADMGDWALRTACDQMAAWHREGLEGLGLSVNISGRQFAGETLVPTVRDALAASGL
                     PASCLELELTETLLMEDTRHSQETIAALKALGVTIALDDFGVGYSSLSYLKQFTLDTL
                     KVDRAFTSEMLTSAESEAIVRATFDIARALRLRTVAEGVETRPQAAFLAELGCDVLQG
                     FYFAKPMPSEQLLAFAKAAPIHLISRPSVAPAAVA"
     gene            complement(3918..6512)
                     /locus_tag="VF453_14860"
     CDS             complement(3918..6512)
                     /locus_tag="VF453_14860"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX7688993.1"
                     /translation="MSIKANVKMVREIPLVDQVEAAREARPVDQAEAARLVASYREHG
                     YLQAAINPLAPTRADDEPAMPRGFRHAAPGAAEDLRGLEQRLRRSYCGGLALDAIHVR
                     SHAQLEWLYARVEAAALEPRPDADARLAIYGQLFAAERLEHAIAAAWPQAKRFSLEGC
                     ESYVVFLRATIEAAAAAQASRVVMGMPHRGRVNVLANVMGLDADDLRSLYTESPAPHL
                     AAWDIKEHLGLTRRVRTAAGAVDVTLAHNPSHLESVVPVIAGMARALQEQSGAQPRKR
                     VVPLVVHGDASFSGQGIVAEALNIAGTRGYNVGGTVHVILNNQIGSTVSNLLDARSTL
                     SSSDIARAYDVPVLHVNGDEPERVVQAARIALEFRQRFHADIVVDLVGYRRHGHNGHD
                     DPRVTQPAMQRGVRARGSVVQRYRERLLEDGVVAGTDVDALERRVLERQSLPIADIDA
                     APAPAPGRGGPASTRESLGVPFAQLRAHVRALCARPEGMRLHDDLHALVRRWQDAAGS
                     IDRRVDWCLAETLAYASLLAAGHHVRLTGLDIGRGSFFHRQCIWHDQDAPADGERLHV
                     PLRGIGEGQGVFSVFDTPLSEEAVLGFEYGYSVMAGDTLVAWEAQFGDFVNNAQVIID
                     QFIASGEAKWGWRSGLAMLLPHGHEGGGPEHTSAYVGRFLALCAERNLVVCMPSTSAQ
                     MFHLLRRQMTDGARKPLVAFTPKGQLYGDETTFSAWREFEDGGFRTVLGEIGAAQAPA
                     VERVVLASGKIAANLAAELAKRPAPRVALLRLEQLYPLPADEIHTRLAACPRLREVVW
                     VQEEARNHGAWAALREPLEEAIAVPGMGIRLRCVARAASAASAGCRRSVHLAEQAGLV
                     EAALRQGL"
     gene            complement(6689..7753)
                     /locus_tag="VF453_14865"
     CDS             complement(6689..7753)
                     /locus_tag="VF453_14865"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7688994.1"
                     /translation="MASLARRLLLAIILAAGTAWGISTARSSAGVDAPLPVEPLHPSF
                     AGATGWLDSPPLTLRSLRGKVVLVDFWTYSCINCLRTLPYVRAWSRTYASQGLVVVDV
                     HAPEFEFEHDPRRVQRALKELQADWPVAIDDDFAIWRSFRNQAWPALYFIDAQGRVRH
                     HRLGEGGYAESERVIRELLEEARADAVALPAAAAVDARGIGQAPDVAHLRSGETYLGY
                     AHADEAAPPDLQPDRAQRHDARPPRLNAWTLGGGWMQRPEFVESTAPDGTIAMRFHAR
                     DLHLVLGTADGRPVGFRVTIDGHPPGGDHGVDVDGQGRGEIAGERLYQLVRERDAVGD
                     HTFEIRFDRAAARAWVFTFG"
     gene            complement(7770..8540)
                     /locus_tag="VF453_14870"
     CDS             complement(7770..8540)
                     /locus_tag="VF453_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7688995.1"
                     /translation="MSRFARALAALAAGLALDAFAADTAPRTDAPPSVVLVHGAFADG
                     SDWARVIPLLQARGVRVTAVQNPLTSLADDVAATKRAIDAAPGKVVLVGHSWGGTVIT
                     EAGASDKVAALVYVSAFAPDVGQNSGELGKGYPAPPGLAALSADASGFLRISDDGMRT
                     HFAFDVPPARAAVMAATQGPIAARAFDEPVDVAAWRTHPSWFVISTRDHMIPPELQRD
                     MARRIGARVTELPTSHVPQEVQPAKVAAAILDAVAHAR"
     gene            complement(8583..>9514)
                     /locus_tag="VF453_14875"
     CDS             complement(8583..>9514)
                     /locus_tag="VF453_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006411604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7688996.1"
                     /translation="PLARAAAAAPAGFPPLRQVDAGPLSIGYAELGPAGGTPVILLHG
                     WPYDIQSFAEVAPRLAQAGHRVIVPYLRGYGSTVFRAADAVRNGQQAALAFDVVALMD
                     ALRIDRAIVAGFDWGARTADIVAALWPQRCVALVSVSGYLIGSPHANAAPLPPAAELA
                     WWYQFYFATERGRVGYERNRVDFNRLIWKTASPTWRFDEAMYQRTAASFDNPDHVAIV
                     IHNYRWRLGLAPGEARYDAAERTLAAAPAIAVPTITMEGDANGAPHPMPEDYRTRFTG
                     RYEHRLVTGGIGHNLPQEAPEAFAQAVLDLARA"
BASE COUNT         1262 a         3479 c         3493 g         1280 t
ORIGIN      
        1 acccggtacg ccgcccgtct cgccggcgag ccgcccggcc gcggcggacg cggagtccct
       61 gcagtacttc cgccgcacct ggtcgcgcct ggccgccgag cagcacctcg cgcaatcgcg
      121 ctccagcctg cccggcaacg cggggccgct gcactcgcac acgctggtgc accgctcgct
      181 ggcgctgatg cgcgagctct cgcccggcta cttcgagcac ttcatggcgc atgtcgacgc
      241 cctggcgtgg ctggaggcgg cggacgaata gccgccatcg cgggcggcca gcggtcgtcc
      301 gcgccggcgg ccggagtcgt tgcccgttcc cgcgcatttg gccggcgtca tgcgtcgtcc
      361 gtctccgcca ggcgttcgag cccgtcgcga tccagccgca tgtgccagcg ctgcgccacg
      421 cggccggcgc ccaacgcacc atagaacgcc tgcgccgccg cgttgccggc ctcgaccgtc
      481 cagtcgatgc gcacgcagcc cagcgcttcg gcgtgccgcg cgacccagcg catcaaggcc
      541 gcgccgacgc cacggccgcg cgcgtcgcgc cgcaggtaga gctccttcag gaacaactac
      601 cgcgaacgct ccgccgagca gtcgaccagc gagtgccacg acagcaccgc cgcgaagccg
      661 agcgcgcgac cgtcgtcggc gaccgccagc gcgaagcgca gcggggagtc cggcatcatc
      721 aggcgctcga cgaggtgcga gcgcaccgct tcgcgcgacg gtgccgcctc gccttggtag
      781 tgcgcgccga ggtcgtgcag caggtcggcg aagcggtcca ccgcctcggg gcggccggtg
      841 tcggaagggg cgaggcaatc gatgcgcatg gccgcgatga tcgtcgatgc gcgcgacgct
      901 cgcgcgggcg ccgcgccact ggcccgccgg cacgcagccg ccgcctcgcc gcgaagccgg
      961 gccgccgctg tagtcgatgc ccgatcgccg cgacgagaaa tcccgacacc aggggacggt
     1021 cgagctgtcg atacacaccg taacgacctt cgcccgagcc accatgtcca acgccgtccc
     1081 ccgttcgctg cctcgcctgc tgcgcttcct cgtcccgcag accctggcca gccgcgcctc
     1141 gctggcgatc tcggcggtgt tcctcgtcgg cgtcgcggcg atggcggtgg tctcgctgtc
     1201 gaccttcgag agccagctcg ccgccgtgct gcgcggcgag caggacctgg tggtcggccg
     1261 catcgccgag gacgtcgacc agcgcatcga gctgctgcag gaggcgttgc gcgacagcgc
     1321 ggcccacatc accgaggccg atctcgccga ctacgccagc gcccgcgcgg cgctcgagcg
     1381 caacgacggc ctcgcggcgg tgttcgaccg ctcggtcttc ctgttctcgg cccggggcgc
     1441 gctggtggcc gagcgcccat accgcaccga tcgcctcggc caggacgcga cctggcgtcc
     1501 ctacatccgc cgcacgatgg acaccgggca gccgcagatc tccgagccgt tcaagaccaa
     1561 catgggcgac ggcaacttcg tgatggtgct gacgtggccc gtgcgcgggc acgacggccg
     1621 cctgatcggc atgatcaccg gcagcctggg tctcacgcgg cccgacctgc tgggcagcgt
     1681 cgcgaagacg cgcttcggcc gcaccggcta cctctcgatc acgaccgcgg acggccgcct
     1741 gctgatcgat ccggagccga gccggctgtc gagcgcgctg tacgcgcccg gcgccaacga
     1801 gcccttcgag cgcgcgctca agggcttcga gggcaccgcg tcgaccgtcg acgagaacgg
     1861 ccgcccgtcc ttcgtcacct accgccgcgt gaagagcgcg ggttggctgg tcgccgccgt
     1921 ctatccgcgc gacgaggcct acgagtacac cgtgcgcttc gtgcgcaact tcctgctcgc
     1981 gctcgccgcc gcggcgctgg cggtgctgtg ggcgatctgg acgctcacgc gctacctcac
     2041 gcggccgctc gatgcgctca gccgccacat cgccgactac acggccaccg agggacgcat
     2101 cgagccgctg gccggcgtcg agggcagcgg cgaggtgcgc gtgctgacga cggcgttcaa
     2161 cgcgctgacg cggcgcctga acgagcgcga ggaatccatg gtcagcgcgc tgcgccagta
     2221 ccaggtcatc accgagagct cgaccgacct catcacgcgc cacgtgcgca acggcacgat
     2281 cgcgttcgcc tcgccggcca gccggcgcgt gctgggcgtc gaggaggccg cgctgcgcgg
     2341 ccgccatgtc ggcgatctcg cgcatcccga ggacctgcag cgcctgctgg ccgccttcga
     2401 cgccgcctgg acgagcgacg gcgcgacggt ggtggcctgg cgcgcccgca cgccggcgga
     2461 cgagcacgtc tggctcgaga gcgcgctgcg cccgctgccg ggcagcggca agggcggcga
     2521 gatcctctgc ctgtcgcgca acatcgacga gcgcaagcgc ctggaggaga agctgcatcg
     2581 cgaggcgcgc ctggaccgcc tgacgcagct gcccaaccgc ctgctgctcg acgagcgcct
     2641 gcccgccgcg atgtcgcgct gccatcggga gggctcgctg ctggccgtgc tgctgatcga
     2701 tctcgaccgc ttcaaggaca tcaacgacac gctcggccac cgccgcggcg acgacctgct
     2761 cgcggccgtc gcggcgcgct tcgcgtcggt cgcgcgcacc ggcgagacgg tcgcgcgctg
     2821 gggcggcgac gagttcgtgg tggtgctgcc ggggctgtcg catccggacg tcgcgcgcct
     2881 ggtcgccgaa cgctttctca aggcgctcaa ggagccgttc gagttcggcg gccaggcgct
     2941 gcaggtctcg gcgtcgatcg gcatcgccgt cacgggcgac gactcggtgg ccaccgagac
     3001 gctgctggcc aacgccgatg tcgcgatgta tcgcgccaag cgccgtggcg gcaacgcctc
     3061 ggtcacgtgg gcttccgaga tgagcgaggg cgcgcaggat cgcctgtcga tggagggtgc
     3121 gctgttccac gccgtcgagc gcgacgagct gcgcctgcac taccagccgc tggtgtccgc
     3181 gcgcaacggc cgcatcgtcg gcgtcgaggc gctgctgcgc tgggagcatc ccgagttcgg
     3241 cctcgtgtcg ccgggcgtct tcatcccgat cgccgagcgc atcggcgtca tcgccgacat
     3301 gggcgactgg gcgctgcgca ccgcctgcga ccagatggcc gcatggcatc gcgagggcct
     3361 cgaggggctg ggcctgtcgg tcaacatctc cggccgccag ttcgcgggag agacgctggt
     3421 gcccacggtg cgcgacgcgc tcgcggcctc cggcctgccg gccagctgcc tcgagctgga
     3481 gctcaccgag acgctgctga tggaagacac ccgccacagc caggagacca tcgccgcgct
     3541 caaggccctg ggcgtcacga tcgcgctcga cgacttcggc gtgggctact cgtcgctcag
     3601 ctatctcaag cagttcacgc tggacacgct caaggtcgac cgcgcgttca cgagcgagat
     3661 gctcacgagc gccgaatccg aggccatcgt gcgcgcgacc ttcgacatcg cgcgcgcgct
     3721 gcggctgcgc acggtggccg aaggcgtcga gacccgcccg caggcggcct tcctcgccga
     3781 gctgggctgc gacgtgctgc agggcttcta cttcgccaag ccgatgccgt ccgagcagct
     3841 cctggccttc gccaaggcgg cgccgatcca cctgatctcg cgcccctcgg tcgcgccggc
     3901 cgcggtggcc tgagcgttca gagcccctgg cgcagcgccg cttcgacgag gccggcctgc
     3961 tcggccaggt gcaccgagcg ccggcagccc gcgctggccg ccgacgccgc gcgcgcgacg
     4021 cagcgcaggc gaatacccat gccgggtacg gcgatcgctt cctccagcgg ctcgcgcagc
     4081 gctgcccacg cgccgtggtt gcgcgcctct tcctgcaccc agaccacctc gcgcagccgc
     4141 gggcaggcgg cgaggcgggt gtggatctcg tcggccggca gcgggtagag ctgttccagc
     4201 cgcagcagcg cgacgcgcgg ggcggggcgc ttcgcgagct cggcggccag gttcgcggcg
     4261 atcttgccgc tcgcgagcac gacgcgctcg accgccggcg cctgcgccgc gccgatctcg
     4321 cccagcaccg tgcggaagcc gccgtcctcg aactcgcgcc aggcggagaa ggtcgtctcg
     4381 tcgccgtaga gctggccctt gggcgtgaag gcgaccagcg gcttgcgcgc gccgtcggtc
     4441 atctggcggc gcagcaggtg gaacatctgc gccgacgtgc tgggcatgca cacgacgagg
     4501 ttgcgctcgg cgcacagggc gaggaagcga ccgacgtagg cgctcgtgtg ctccgggccg
     4561 ccgccctcgt ggccatgcgg cagcagcatc gccaggccgc tgcgccagcc ccacttggcc
     4621 tcgcccgagg cgatgaactg gtcgatgatc acctgtgcgt tgttgacgaa gtcgccgaac
     4681 tgcgcctccc acgcgacgag cgtgtcgccc gccatcaccg agtagccgta ctcgaagccg
     4741 agcaccgcct cctccgacag cggcgtgtcg aacacggaga acacgccctg gccctcgccg
     4801 atgccgcgca gcggcacgtg caggcgctcg ccgtcggccg gcgcgtcctg gtcgtgccag
     4861 atgcactggc gatggaagaa gctgccgcga ccgatgtcca gcccggtgag ccgcacatgg
     4921 tggccggccg cgagcagcga cgcgtaggcg agcgtctccg cgaggcacca gtcgacgcgg
     4981 cggtcgatgc tgcccgccgc atcctgccag cgccgcacca gcgcgtggag gtcgtcgtgc
     5041 aggcgcatgc cttcgggccg cgcgcacagc gcccggacgt gcgcccgcag ctgcgcgaac
     5101 ggcacgccca ggctttcgcg cgtcgacgcc ggcccgccgc ggccgggcgc gggggccggc
     5161 gcggcgtcga tgtcggcgat cggcagcgac tggcgctcga gcactcggcg ctcgagcgcg
     5221 tcgacgtccg tgcccgcgac cacgccgtcc tccagcagcc gctcgcgata gcgctgcacg
     5281 acgctgccgc gcgcgcgcac gccgcgttgc atcgcgggct gcgtcacgcg cgggtcgtcg
     5341 tggccgttgt ggccgtggcg gcggtagccc accaggtcga cgacgatgtc ggcgtggaag
     5401 cgctggcgga actccagcgc gatgcgggcc gcctgcacca cgcgctcggg ctcgtcgccg
     5461 ttcacgtgca gcacgggcac gtcgtaggcg cgcgcgatgt ccgagctgct gagggtcgag
     5521 cgtgcgtcga gcaggttgga gaccgtcgag ccgatctggt tgttcaggat gacgtgcacc
     5581 gtgccgccga cgttgtagcc gcgcgtgccg gcgatgttga gcgcctcggc gacgatgccc
     5641 tggccgctga acgacgcgtc gccatgcacg acgagcggca ccacgcgctt gcgcggctgc
     5701 gcgcccgact gttcctgcag cgcgcgcgcc atgccggcga tcacgggcac cacgctctcg
     5761 aggtgagacg ggttgtgcgc gagcgtcacg tccaccgcgc cggccgcggt gcgcacgcgc
     5821 cgcgtgagac ccaggtgctc cttgatgtcc caggccgcca ggtgcggcgc cggggactcc
     5881 gtgtacagcg agcgcaggtc gtcggcgtcc aggcccatca cgttggcgag cacgttgacg
     5941 cggcctcggt gcggcatgcc catgacgacg cggctcgcct gcgcggcggc ggcggcctcg
     6001 atcgtcgcgc gcaggaagac gacataggac tcgcagccct cgaggctgaa gcgcttggcc
     6061 tgcggccagg ccgcggcgat cgcgtgctcc aggcgctccg ccgcgaacaa ctggccgtag
     6121 atcgcgagcc gggcatcggc atcggggcgc ggctcgagcg cggccgcctc gacgcgggcg
     6181 tacagccatt cgagctgcgc gtggctgcgc acgtggatgg cgtccagcgc gagcccgccg
     6241 caatagctgc ggcgcaggcg ttgctcgagg ccgcgcaggt cctcggcggc gccgggcgcg
     6301 gcgtggcgga agccgcgcgg catcgccggc tcgtcgtcgg cgcgcgtcgg cgcgagcgga
     6361 ttgatcgccg cctgcaggta gccgtgctcc cgatagctcg cgaccagccg cgcggcctcc
     6421 gcctgatcca cgggcctcgc ctcgcgcgcg gcctcgacct gatccaccag cgggatctcc
     6481 cgcaccatct tcacgttcgc cttgatcgac atgtcgcctt ttgcctgcac atgcagatgg
     6541 cgccacgtcg atccaccacc cgcctaatcg aattcccggg agccgtggcc gggagatggg
     6601 cggattttcg tgacgtagtt gcggatcgcc tgtcgggaca tctcgacgtc ggcgcgggga
     6661 acttctcgac gcgtcggacg cgcgcgcgtc agccgaacgt gaagacccag gcgcgcgcgg
     6721 cggcgcggtc gaagcggatc tcgaaggtgt ggtcgcccac cgcgtcgcgc tcgcgcacga
     6781 gctggtacag gcgctcgccc gcgatctcgc cgcggccctg tccgtcgacg tcgacgccat
     6841 ggtccccgcc cggcgggtgg ccgtcgatcg tcacccggaa gccgaccggc ctgccgtcgg
     6901 cggtgcccag caccaggtgc aggtcgcgcg catggaagcg catcgcgatc gtgccgtcgg
     6961 gcgcggtgga ctccacgaat tcgggccgct gcatccagcc tccgcccagc gtccaggcgt
     7021 tcagcctcgg cggccgcgca tcgtggcgtt gcgcgcgatc gggctgcagg tcgggcggcg
     7081 cggcctcgtc ggcgtgggca taccccaggt acgtttcgcc cgaacgcagg tgcgcgacgt
     7141 cgggcgcctg gccgatgccg cgtgcgtcga cggcggcggc cgcgggcagg gccaccgcat
     7201 cggcgcgtgc ctcctccagc agctcgcgga tgacgcgctc ggattcggcg tagccgccct
     7261 cgccgagccg gtggtggcgc acgcgcccct gcgcgtcgat gaagtacagc gccggccagg
     7321 cctggttgcg gaacgagcgc cagatggcga agtcgtcgtc gatcgcgacg ggccagtcgg
     7381 cctgcagctc cttcagcgcg cgctggacgc gccgcgggtc gtgctcgaac tcgaactccg
     7441 gcgcatgcac gtcgaccacc acgaggccct ggcttgcgta ggtgcgcgac cacgcgcgca
     7501 catacggcag cgtgcgcagg cagttgatgc aggagtaggt ccagaagtcc accagcacga
     7561 ccttgccgcg cagcgaccga agcgtcagcg gcggggagtc cagccagccg gtggcgccgg
     7621 cgaagctggg gtgcagcggt tcgaccggca gcggcgcgtc gacgcccgcg gacgaacgcg
     7681 ccgtcgagat gccccaggcc gtacccgcgg ccaggatgat cgcgagcagc agccgacggg
     7741 cgagcgatgc catgcgcggc tccttcgcgt cagcgcgcgt gcgccacggc gtcgaggatg
     7801 gcggccgcga ccttcgcggg ctgcacctcc tgcggtacgt ggctggtcgg cagctcggtg
     7861 acgcgcgcgc cgatgcgcct ggccatgtcg cgctgcagct cgggcgggat catgtggtcg
     7921 cgcgtcgata tcacgaacca gctcggatgc gtcctccagg ccgcgacgtc caccggctcg
     7981 tcgaaggccc tggcggcgat cgggccctgg gtcgcggcca tcaccgcggc gcgggcgggc
     8041 gggacgtcga acgcgaagtg cgtgcgcatg ccgtcgtcgc tgatgcgcag gaagccggac
     8101 gcgtctgcgc tcagcgcggc caggcccggc ggcgccggat agcccttgcc cagctcgccc
     8161 gagttctggc cgacgtccgg cgcgaacgcg ctcacgtaga ccagcgcggc caccttgtcg
     8221 ctcgcacccg cctcggtgat gacggtgccg ccccaggagt gcccgaccag caccaccttg
     8281 cctggcgcgg cgtcgatggc gcgcttcgtg gcggcgacgt cgtcggcgag cgatgtcagc
     8341 gggttctgca ccgcggtgac gcgcacgccg cgcgcctgca acagcgggat gacgcgcgcc
     8401 cagtcggagc cgtcggcgaa ggcgccgtgg acaaggacga ccgagggcgg ggcgtccgta
     8461 cggggggccg tatcggcggc gaaggcgtcg agggcgaggc cggcggcgag ggcggcgagg
     8521 gcgcgggcga agcgggacat ggattgttcc ttggcgaggg agggggcgga agggcggcgc
     8581 cgtcaggcgc gcgcgaggtc gaggacggcc tgcgcgaagg cctcgggcgc ttcctgcggc
     8641 aggttgtggc cgatgccgcc ggtgacgagg cggtgctcgt agcggccggt gaagcgggtg
     8701 cgatagtcct cgggcatcgg atgcggcgcg ccgttcgcgt cgccctccat cgtgatggtc
     8761 ggcaccgcga tcgcaggcgc cgcggcgagc gtgcgctcgg cggcgtcgta gcgcgcctcg
     8821 ccgggcgcga ggcccaggcg ccagcggtag ttgtggatca cgatggcgac gtggtcgggg
     8881 ttgtcgaacg atgccgcggt gcgctggtac atggcctcgt cgaagcgcca ggtcggcgac
     8941 gccgtcttcc agatgaggcg gttgaagtcg acgcggttgc gctcgtagcc gacgcggccg
     9001 cgctcggtcg cgaagtagaa ctggtaccac cacgcgagct cggcggccgg cggcagcggg
     9061 gccgcgttgg cgtgcgggct gccgatcagg tacccgctca ccgagacgag cgccacgcag
     9121 cgctgcggcc acagcgcggc gacgatgtcg gccgtgcgcg cgccccagtc gaagccggcg
     9181 acgatcgcgc ggtcgatccg cagcgcgtcc atcagcgcga cgacgtcgaa ggcgagtgcc
     9241 gcctgctggc cgttgcgcac cgcgtcggcc gcgcggaaca cggtcgatcc atacccccgg
     9301 aggtagggaa cgatcacgcg gtggcccgcc tgcgccaggc gcggcgcgac ttcggcgaag
     9361 ctctggatgt cgtagggcca gccgtgcagc aggatcaccg gcgtgccgcc cgccggcccg
     9421 agctcggcgt agccgatcga gagcgggccg gcatcgacct ggcgcagcgg cggaaagccc
     9481 gcgggggcgg ctgccgcggc gcgcgcgagc ggcg
//
LOCUS       DASVLN010000620         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_247116, whole genome shotgun sequence.
ACCESSION   DASVLN010000620 DASVLN010000000
VERSION     DASVLN010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247116"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1186)
                     /locus_tag="VF453_14880"
     CDS             complement(125..1186)
                     /locus_tag="VF453_14880"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX7688997.1"
                     /translation="MPSTAPPVPPVEPMLAKIADALPDAPGFQFEPKWDGFRAIAFRT
                     AQGLLLQSRDLKPLNRYFPELERALLDALPEGCVLDGEIVVATPRGLDFDALQQRLHP
                     ARSRVERLSRETPARFVAFDLLAVDGQSTMALAQAERRVLLEGLLTGAQPPLHLTPAT
                     TDRAQAQRWLAEFEGAGLDGVVAKIESAAYQPGKRAMLKIKHVRSADCVVAGFRWYKD
                     RDDAVGSLLLGLYDDAGALRHVGVTSSFTLAMRRALLAELAPLREDARATHPWAAALD
                     EGSGPGATSRWSRGKDLSWEPLRPERVCEVRYDHLQGARFRHATTFLRWRPDKPPRDC
                     RYDQLEVTTPYELARVFGA"
     gene            1267..1476
                     /locus_tag="VF453_14885"
     CDS             1267..1476
                     /locus_tag="VF453_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7688998.1"
                     /translation="MTDPQRPRDRDGPRRAAPLQPDKPGSGNRRESPGEQPGASTDEH
                     LDRDATRRASEQEHAAVDNTREGYR"
     gene            complement(1536..2084)
                     /locus_tag="VF453_14890"
     CDS             complement(1536..2084)
                     /locus_tag="VF453_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7688999.1"
                     /translation="MTLLDKLMASTTPGPSDEERTRTRHIARLLADDHPWLDEVIRHH
                     VAIEDALDNVRLAADAPGRRHALRWLRTLLTGHFLAEETVLYPAMAQDGLRVHAVTAY
                     ADDSGTKIELAALETMDVRGPDFADKLERLRGELAWHLHEEETTWFPALCRAGGAAFH
                     GRLTGRFVAEFERYMGVDGDLL"
     gene            2457..>2740
                     /locus_tag="VF453_14895"
     CDS             2457..>2740
                     /locus_tag="VF453_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689000.1"
                     /translation="MTSKQQGNQGNQGHQGGQGGQGGQHGASGDQHRGRQSEQSSDRD
                     EQQGGRGQASHGGGHQASQGGSHGGQHGSSGGQHGSHEGGSRQSQQESSGG"
BASE COUNT          384 a         1001 c         1040 g          315 t
ORIGIN      
        1 cgccgcgcga cgtgctcgtc gaccgcatgc gcgggcgctc gtcgctctgg taccgcgtgc
       61 gggtcaacct ggcgcgcgtg ccggagccgc tgcgtccgct ggcggcgccg gcgcgcgacc
      121 gctctcaggc gccgaagacc cgcgcgagct cgtagggcgt ggtgacctcc agctggtcgt
      181 agcggcagtc gcggggcggc ttgtcgggcc gccagcgcag gaaggtcgtc gcgtggcgga
      241 agcgcgcgcc ctgcaggtgg tcgtagcgca cctcgcagac gcgctcgggg cgcagcggct
      301 cccagctcag gtccttgccc cggctccagc ggctggtcgc gccggggccg gatccctcgt
      361 cgagcgccgc cgcccacgga tgcgtcgcgc gcgcgtcctc gcgcagcggc gccagctcgg
      421 cgaggagcgc gcgccgcatc gccagggtga acgaggacgt gacgcccacg tgccgcagcg
      481 cgcccgcgtc gtcgtacagg cccagcagca gcgagccgac ggcgtcgtcg cgatccttgt
      541 accagcggaa cccggcgacg acgcagtcgg cgctgcgcac gtgcttgatc ttgagcatcg
      601 cgcgcttgcc cggctggtag gcggccgact cgatcttcgc gacgacgccg tccaggccgg
      661 cgccctcgaa ctcggcgagc cagcgctgcg cctgcgcgcg gtcggtggtg gcgggcgtca
      721 gatgcagcgg aggctgcgcc ccggtcagca ggccctcgag cagcacgcgc cgctcggcct
      781 gcgcgagcgc catcgtcgac tggccgtcca ccgccagcag gtcgaacgcg acgaagcgcg
      841 cgggcgtctc gcgcgacagg cgctcgacgc gcgagcgcgc cgggtgcagg cgctgctgca
      901 gggcgtcgaa gtccaggccg cgcggcgtcg cgacgacgat ctcgccgtcc agcacgcagc
      961 cctcgggcag cgcgtcgagc agggcgcgct cgagctcggg gaagtagcgg ttcagcggct
     1021 tcaggtcgcg gctctgcagc aggaggccct gcgcggtgcg gaacgcgatc gcgcggaagc
     1081 cgtcccactt cggctcgaac tggaagcccg gcgcgtcggg cagggcgtcg gcgatcttcg
     1141 cgagcatcgg ctcgacgggc ggcacggggg gcgcggtgga cggcatggtg gccgagcggc
     1201 aggaagcgtg cgagccacgg ccggcgagga acgggccttg ccgcgccagg ccttggaggt
     1261 gatcccatga ccgatccgca acgaccccgc gaccgcgacg gcccgcgccg cgccgcgccc
     1321 ctgcaacccg acaagcccgg cagcggcaac cgccgggaga gccccggcga gcagcccggg
     1381 gcctcgaccg acgaacatct cgaccgcgac gcgaccaggc gcgccagcga acaggagcac
     1441 gccgccgtcg acaacacgcg cgagggctat cgctagccgc ggcgaaatga atccggcacg
     1501 cccgccgggc ggcgccgggc cgcccgccgt ggcactcaca ggaggtcgcc gtcgacgccc
     1561 atgtagcgct cgaactcggc cacgaagcgg ccggtcagcc ggccatggaa ggcggcgccg
     1621 ccggcgcggc acaacgcggg gaaccaggtc gtctcctcct cgtgcaggtg ccaggcgagc
     1681 tcgccgcgca ggcgctcgag cttgtcggcg aaatccggcc cgcgcacgtc catcgtctcg
     1741 agtgcggcca gctcgatctt cgtgccgctg tcgtccgcat aggcggtgac ggcgtggacg
     1801 cgcagcccgt cctgcgccat cgccgggtag agcacggtct cctcggcgag gaaatgcccc
     1861 gtcaacagcg tgcgcagcca gcgcagcgca tggcggcggc cgggcgcgtc cgccgcgagc
     1921 ctcacgttgt ccagggcgtc ctcgatcgcg acgtggtggc ggatcacctc gtcgagccac
     1981 ggatggtcgt cggccagcag gcgggcgatg tggcgggtgc gcgtgcgctc ctcgtccgag
     2041 ggcccgggcg tcgtcgacgc catcagcttg tccagcaaag tcatgtgcac ctcctgcagt
     2101 gaaaacgacc gcatcgcgcg gcccggggcg tcgccggcat gcgggccggg ccggggcgca
     2161 agcgtcgtgc cgcgacctcg acgggccgcg ggcgcgttgc ggacgacgcg cggcttcaaa
     2221 tcggctgaag ccgcggcacg aggcgccatc cgccgacgaa gcggcctgcc gcgcccgcgc
     2281 ggcgggcctg gcttcgaagc cgctgaagct gcaaatcgcg ctgcgccggc aatccgttcg
     2341 cggcgagatg cccgacgccg tgcgtgcggc gcagcctgcg cgtcggggta tgcgcgttgc
     2401 ttcacccagg ccgtgagcgg cctccgctcg tcatccacgc aaccaaggag cattccatga
     2461 ccagcaagca gcaaggcaat cagggcaacc aagggcatca gggcggccag ggcggccagg
     2521 gcggccagca cggtgcgagc ggcgaccagc accgcggccg gcagtccgag cagtcttccg
     2581 accgggacga gcagcagggc ggtcgcggcc aggcctcgca cggcggcgga caccaggcct
     2641 cgcagggcgg atcgcacggc ggccagcacg gctcgtcggg cggccagcac ggttcgcatg
     2701 aaggcggttc gcgccagtcg cagcaggagt cgtccggcgg
//
LOCUS       DASVLN010000621         3962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_86884, whole genome shotgun sequence.
ACCESSION   DASVLN010000621 DASVLN010000000
VERSION     DASVLN010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86884"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="nagZ"
                     /locus_tag="VF453_14900"
     CDS             complement(<1..1055)
                     /gene="nagZ"
                     /locus_tag="VF453_14900"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX7689001.1"
                     /translation="MSAAPDTFHPGHLVMVDIPGTALDAATADFLRRHRVRAVCLFRR
                     NLGTEAEVRRLTADLREAMGPHALIGIDQEGGSVVRATFLPQAPAAMALGAVGDEALA
                     EEVGAAVARGLRSLGINWNFAPVLDVNNNPANPVIAERSFGEDPDAVARLAAAWMRGS
                     LREGVACCVKHFPGHGDTHVDSHHALPTVDKSLEELERLELRPFRALAHASGATPAAP
                     AVMTAHIVYPQIDPDHPATLSRPILTGLLREAIGYDGVVLTDALFMKAIHEKYGHALG
                     AVMALQAGADMPLAQGSLEEQSAAVKAIEAALHQGHLLPADVKRAQARLVKLAEDYPV
                     APRDYPAAQRAADDAAMR"
     gene            complement(1150..2160)
                     /locus_tag="VF453_14905"
     CDS             complement(1150..2160)
                     /locus_tag="VF453_14905"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7689002.1"
                     /translation="MFDEAREAPAAVARLMAADQAAYTSLGEQLRADPPRSALTVARG
                     SSDHAAHYMAYLVMARLGRLVTSLPMSLVTLYQSRLQCDGLVSWGFSQSGQSPDLIAP
                     TRFFRDGGARTVAVVNDAGSPLAEAAQWVLPLHAGAETSVAATKSYIAQLVAGARIVA
                     NWQDDQALLAALETLPEVLERAAYADWQPLVDALVDADKLFVIGRGTSLAIAMEAALK
                     FKEVCGIQAEAFSGAEVKHGPMALIEEGYPLLVFAPRGPAQAGLLALAEEMRRRGARV
                     LVAAPVGTPGAELPLVVAGHEDLDPISAIQSYYLAVEALARARGLDPDKPRHLNKVTL
                     TH"
     gene            complement(2153..3289)
                     /gene="nagA"
                     /locus_tag="VF453_14910"
     CDS             complement(2153..3289)
                     /gene="nagA"
                     /locus_tag="VF453_14910"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX7689003.1"
                     /translation="MSVQAQRLSGHVLTPQGFVRGTLDIAADGRIASIDGQPVSEGEA
                     RLSGDPLILPGFIDLHVHGGGGHDTMEGGRAVFEIARLHAKHGTTSMLATTMTAPMSD
                     ISAAMHALAEACRTRQAGGADVMGVHLEGPYINPGALGAQPDFARPASVDEIKALHAI
                     APIRLITMAPELPGNLDLIAPLRAAGFHVQIGHTAGTYEDGVAALQRGATGFTHLFNA
                     MSKFKHRDPGIAGAALAHARYAEIIPDLLHVHPGAIRAALRAIPCLYCVTDSTAAAGM
                     PDGDYNLGSHRVTKCLGGVRLADGTLAGSTLTMDQALRNLVGMGLSVEDASMRVSTHA
                     ADFLGLADRGRLLVGARADLVVLDGELQLNQVIVEGDQIELTDV"
     gene            complement(3286..>3962)
                     /locus_tag="VF453_14915"
     CDS             complement(3286..>3962)
                     /locus_tag="VF453_14915"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7689004.1"
                     /translation="CVLGLGLAGAVAPHLAAAFFRAAPEYALIVLETDSRTALMGAHA
                     GGPGSIVAVGTGAVGEALHADGRRVAVSGWGFPVGDEGSGAWLGLRAMQIAHRALDGR
                     APAGTLAQAIWRRISDRRDTLLEWCSRAGQHDYAQLAPLVFECADTDAAAQALVRDIG
                     QEIEDMALALDPGLQLPIVLLGSIGQRMADRLPAALRARIVEPSGDAVDGAHRLVRQQ
                     MKEAVA"
BASE COUNT          506 a         1398 c         1521 g          537 t
ORIGIN      
        1 cgcatggcgg cgtcgtcggc ggcgcgctgg gcggcggggt agtcgcgcgg cgcgacgggg
       61 tagtcctcgg ccagcttcac gagccgcgcc tgcgcgcgct tgacgtcggc cggcagcagg
      121 tggccctggt gcagcgcggc ctcgatcgcc ttgacggcgg cggactgctc ctcgaggctg
      181 ccctgcgcga gcggcatgtc cgcgcccgcc tgcagcgcca tgacggcgcc gagcgcgtgc
      241 ccgtacttct cgtggatcgc cttcatgaac agggcgtcgg tgaggacgac gccgtcgtag
      301 ccgatcgcct cgcgcagcag gccggtgagg atgggccgcg acagcgtggc cggatggtcg
      361 ggatcgatct gcgggtagac gatgtgcgcc gtcatcacgg ccggggccgc gggcgtcgcg
      421 ccgctcgcgt gggcgagcgc gcggaacggg cgcagctcga ggcgctcgag ctcctcgagc
      481 gacttgtcga cggtcggcag cgcgtggtgc gagtcgacgt gcgtgtcgcc gtggccgggg
      541 aagtgcttga cgcagcaggc cacgccttcg cgcagcgagc cgcgcatcca cgccgcggcg
      601 aggcgggcga cggcgtccgg gtcctcgccg aagctgcgtt ccgcgatcac cgggttggcc
      661 gggttgttgt tcacgtccag gacgggcgcg aagttccagt tgatgcccag gctgcgcagc
      721 ccgcgcgcga ccgccgcgcc gacctcctcg gccagggcct cgtcgccgac ggcgcccagc
      781 gccatcgcgg ccggcgcctg cggcaggaag gtcgcgcgca cgacggagcc gccctcctgg
      841 tcgatgccga tcagcgcgtg cgggcccatc gcctcgcgca ggtcggcggt gagccggcgc
      901 acctcggctt cggtgccgag gttgcggcgg aacaggcaca ccgcgcgcac gcgatggcgg
      961 cgcaggaaat ccgcggtggc cgcgtcgagc gcggtgccgg ggatgtcgac catcaccaga
     1021 tggccgggat ggaacgtgtc gggggccgcg ctcatgtcgt gtgatcgatg tgtgtcgaca
     1081 gggggtgttc aggcggcgtg ccgcatgcag gccgcacgcc tgcgagcgca gcgcgcggga
     1141 atcgtcatgt caatgcgtca acgtgacctt gttgaggtgg cgcggcttgt cgggatcgag
     1201 gccccgcgcg cgcgcgagcg cctcgacggc caggtagtag ctctggatcg cggagatcgg
     1261 gtcgaggtcc tcgtggccgg cgacgaccag cggcagctcc gcgcccggcg tgcccaccgg
     1321 cgccgcgacg agcacgcgcg cgccgcgccg gcgcatctcc tcggcgagcg cgagcaggcc
     1381 ggcctgggcc gggccgcgcg gcgcgaacac gagcaggggg tatccctcct cgatcagcgc
     1441 catcggaccg tgcttgacct cggcacccga gaaggcctcg gcctggatgc cgcagacctc
     1501 cttgaacttc agcgccgcct ccatcgcgat cgccaggctc gtgccgcggc cgatgacgaa
     1561 cagcttgtcc gcgtcgacca gcgcgtcgac cagcggctgc cagtcggcgt aggcggcgcg
     1621 ttcgagcacc tcgggcagcg tctccagcgc cgcgagcagc gcctggtcgt cctgccagtt
     1681 ggcgacgatg cgcgctccgg cgacgagctg ggcgatgtag ctcttggtcg cggcgacgct
     1741 ggtctcggcg ccggcgtgca gcggcagcac ccactgcgcg gcctcggcca gcggcgagcc
     1801 ggcgtcgttg acgaccgcga ccgtgcgcgc gccgccgtcg cggaagaagc gcgtcggcgc
     1861 gatcaggtcg gggctctggc ccgactgcga gaagccccag gacacgagcc cgtcgcactg
     1921 caggcgcgac tggtacagcg tgacgagcga catcggcagc gaggtgacca gccgcccgag
     1981 ccgcgccatc acgagatagg ccatgtagtg cgccgcgtgg tcggagctgc cgcgcgcgac
     2041 ggtgagggcc gaccgcgggg gatctgcacg gagttgctcg ccgagcgagg tgtaggcggc
     2101 ctggtccgcc gccatcagac gggcgaccgc ggccggagcc tcgcgcgcct cgtcaaacat
     2161 ccgtgagctc aatctggtct ccttcgacga tcacttggtt caattgcagc tcgccatcca
     2221 gcacgacgag gtcggcgcgc gcgcccacca gcagccggcc gcggtcggcg agcccgagga
     2281 agtcggcggc atgcgtcgag acgcgcatcg aggcgtcctc gacgctcagg cccatgccga
     2341 cgaggttgcg cagcgcctgg tccatcgtca gcgtgctgcc ggccagcgtg ccgtcggcca
     2401 ggcggacccc gcccaggcac ttggtcacgc gatggctgcc gaggttgtaa tcgccgtcgg
     2461 gcatgccggc cgcggcggtg gagtcggtca cgcagtacag gcaggggatc gcgcgcagcg
     2521 ccgcgcggat cgcgcccggg tgcacgtgca gcaggtcggg gatgatctcg gcgtagcgcg
     2581 catgcgcgag cgccgcgccg gcgatgccgg ggtcgcggtg cttgaacttg ctcatcgcgt
     2641 tgaacaggtg cgtgaagccg gtcgcgccgc gctgcagggc ggcgacgccg tcctcgtagg
     2701 tgccggcggt gtggccgatc tgcacgtgga agccggccgc gcgcagcggc gcgatgaggt
     2761 cgaggttgcc gggcagttcg ggcgccatcg tgatcaggcg gatcggcgcg atcgcgtgca
     2821 gcgccttgat ctcgtcgacc gacgcgggcc gcgcgaagtc gggctgggcg ccgagcgcgc
     2881 cggggttgat gtacgggccc tcgaggtgca cgcccatcac gtccgccccg ccggcctggc
     2941 gcgtgcgaca ggcctcggcg agcgcgtgca tcgcggcgga gatgtccgac atcggcgccg
     3001 tcatcgtggt cgcgagcatc gacgtcgtgc cgtgtttcgc gtgcaggcgt gcgatctcga
     3061 agacggcgcg gccgccttcc atcgtgtcgt ggccgccgcc gccatgcacg tgcaggtcga
     3121 tgaagccggg caggatcagc gggtcgcccg acagccgcgc ctcgccctcg ctgaccggct
     3181 ggccgtcgat gctcgcgatg cggccgtcgg cggcgatgtc cagcgtgccg cgcacgaagc
     3241 cctgcggcgt gaggacgtgg cccgagagcc gctgggcttg gacgctcatg ccaccgcttc
     3301 cttcatctgc tggcgcacga gccggtgcgc gccgtcgacg gcgtcgcccg agggctcgac
     3361 gatccgcgcc cgcagcgcgg ccggcagccg gtccgccatc cgctgtccga tgctgcccag
     3421 cagcacgatg ggcaattgga gtcccgggtc cagcgcaagc gccatgtctt cgatttcctg
     3481 gccgatgtcg cgcacgaggg cctgcgccgc cgcgtcggtg tccgcgcact cgaagacgag
     3541 cggggcgagc tgggcatagt cgtgctggcc ggcgcgcgag caccattcca gcagcgtgtc
     3601 gcggcggtcg ctgatgcggc gccagatcgc ctgggcgagc gtgccggccg gcgcgcggcc
     3661 gtcgagcgcg cggtgcgcga tctgcatggc gcgcaggccc agccacgcgc cgctgccttc
     3721 gtcgccgacc gggaagcccc agccgctgac cgccacgcgc cgtccgtcgg cgtgcagcgc
     3781 ctcgccgacc gcgccggtac ccaccgcgac gatggagccg ggccccccgg cgtgggcgcc
     3841 catgagggcg gtgcgggagt ccgtctccag cacgatcagc gcgtactcgg gcgcggcgcg
     3901 gaagaacgcg gcggcgagat gcggcgcgac cgcgcccgcc aggcccagcc cgagcacgca
     3961 ct
//
LOCUS       DASVLN010000622         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_193682, whole genome shotgun sequence.
ACCESSION   DASVLN010000622 DASVLN010000000
VERSION     DASVLN010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193682"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VF453_14920"
     CDS             complement(<1..408)
                     /locus_tag="VF453_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004534529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX7689005.1"
                     /translation="MNPSPLSSRLVAAFAVLALIATAAGAAQDGPPAPRACTARSGPH
                     VPAVVELYTSEGCSSCPPAERWLARLAGRDDVIALAFHVDYWDGLGWKDRFAQPQFTQ
                     RQDASRRTSGARFAYTPQVILDGRDTPAWSALGP"
     gene            complement(528..2540)
                     /locus_tag="VF453_14925"
     CDS             complement(528..2540)
                     /locus_tag="VF453_14925"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7689006.1"
                     /translation="MRTILRGLAIAVAAACTMPVLAEDHAPPALRVDPLVEQFLEASA
                     IVEASLSPDGRYILAIVALDTQREAVLVDTLGHKSTIVMPATEMPLSGARWFAPDLMG
                     FNTVDQGGARGYVVDLKDGQTRRLEPGAVFISSLHDAAGHPTEWALSTTASTMHDIER
                     VDVRTLEHVPYEFDIPGQRLAYAVTDRRGDLLAVQTTDSALWSDNTRVTVWYRADTSA
                     AWSKVDERRVNDEVLLPVAVLADKGHLAVLTRHGGDRMALWDYDVGTHALVRQLAADE
                     HDDIDAIGIDGMNPDSIVLRTAGLKPRMLWLDPWLAGMQAAIDEQLPGRVNTLSNTSA
                     ERVLVRSESDVDPGQLYVFDLKAMTLTRVLSVRHDIVPGRMQPKQAIHYPARDGLSIP
                     AYLTLPGKQAGPLPLVVLVHGGPIARDQWTWDEDVQMLAAHGYAVFQPQFRGSTGFGA
                     AFEAAGHGQAGLAMQDDISDGVRWLIAQKIADPKRVCIVGISYGGYAALWGLASTPEL
                     YRCGVSVSGPSDLEREITEESLGSLSRPVRERWKLVFGERDRMKATWAAVSPLRHADR
                     IQSPVLLVHGERDAIVPISHGERMRDALLDLHRDVEWLSFDLEGHGVRLATDRRLWYG
                     AMLELLARTIGEGTPPLPPPPSSIDAARDIARRAGLQTWIPYGPRP"
     gene            complement(2653..>3504)
                     /locus_tag="VF453_14930"
     CDS             complement(2653..>3504)
                     /locus_tag="VF453_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX7689007.1"
                     /translation="GFALGPRLNAAGRLADMTLGIECLLTDDASRAAALAAELDRINR
                     ERRDVEAGMREQAEALVERVMPDGAAPAALALFHEDFHEGVVGIVAGRVKDRLHRPTF
                     VFAVGADGLLKGSGRSIAGFHLRDALDLVSKRHPGLLRKFGGHAMAAGATLAREDFAT
                     FDRAFAAVAEEWLGPAQLARVLATDGPLAHEWFNAVTVAALDAQVWGQGFEAPTFCDE
                     VDVVQQRLVGEKHLKLRVRHGGTLRDAIWFGHAEPVADRVRIAYRLSLDEFQGQQRVQ
                     MVIEAAG"
BASE COUNT          498 a         1236 c         1266 g          504 t
ORIGIN      
        1 cggcccgagc gccgaccagg ccggcgtgtc ccgcccgtcg aggatcacct gcggcgtata
       61 ggcgaagcgt gcgccgctcg tgcgccgcga ggcgtcctgg cgctgcgtga actgcggctg
      121 cgcgaagcgg tccttccagc cgaggccgtc ccagtagtcg acgtggaagg cgagcgcgat
      181 cacgtcgtcc cgacccgcga gccgggccag ccagcgctcg gctggcggac acgaactgca
      241 gccctccgac gtgtagagct cgaccacggc cggcacgtgc gggcccgagc gggcggtgca
      301 cgcgcgaggt gccggaggtc catcctgggc cgcgccggcg gccgtggcga tcagcgcaag
      361 cacggcaaat gccgcgacga gccggctcga taagggagaa gggttcatgg cgtcaggatg
      421 acggtgatga ctcgcgttgg tcgaaccccg gggccggagc cttacacatt ggtgccggct
      481 cgagcgtgcg tcggcagtcg cgcagtcgag cgcccgcggc gcctgcttca cgggcgaggt
      541 ccgtagggaa tccaggtttg cagtccggcg cgccgggcaa tgtcccgggc cgcgtcgatc
      601 gacgacgggg gcggcggcag aggcggcgtt ccctcgccga tcgtgcgggc cagcagctcc
      661 agcatcgcgc cgtaccacag gcggcggtcg gtcgcgaggc ggacgccgtg gccctcgagg
      721 tcgaaggaaa gccactcgac gtcgcgatgc aggtcgagga gcgcgtcgcg catgcgttcg
      781 ccgtgagaga tcggcacgat ggcgtcgcgc tcgccatgta ccagcagcac cggcgactgg
      841 atgcgatcgg cgtggcgcag cggcgagacg gccgcccacg tggccttcat gcggtcgcgc
      901 tcgccgaaga ccagtttcca ccgctcgcgt accggccgcg aaaggctgcc cagagactcc
      961 tcggttatct cgcgctcgag gtccgacggc cccgacaccg agacgccgca gcggtagagt
     1021 tccggggtgg atgccagtcc ccacagcgcc gcataaccgc catagctgat gccgacgatg
     1081 cagacacgct tcggatccgc gatcttctgc gcgatcagcc agcgcacgcc gtccgagatg
     1141 tcgtcctgca tcgcgaggcc cgcctggccg tggccggcgg cctcgaacgc ggcgccgaac
     1201 ccggtcgagc cgcggaattg aggctggaac accgcgtagc cgtgcgccgc gagcatctgc
     1261 acgtcctcgt cccatgtcca ctggtcccgt gcgatcgggc cgccgtgcac gagtaccacc
     1321 agcggcaggg gcccggcctg tttgcccggc agcgtgaggt acgccgggat ggacaggccg
     1381 tccctggccg ggtaatggat cgcctgcttc ggctgcatgc ggcccgggac gatgtcgtga
     1441 cgcacggaca gtacccgcgt caaggtcatc gccttgaggt cgaacacgta aagctgcccc
     1501 gggtccacgt ccgattccga gcgcaccagc acgcgttccg cgctcgtgtt gctcagcgtg
     1561 ttgacgcggc caggcaactg ctcgtcgatc gcggcctgca tgcctgccag ccaggggtcg
     1621 agccacagca tgcgtggctt cagccccgcg gtgcgcagga cgatcgagtc cgggttcatg
     1681 ccgtcgatgc cgatggcgtc gatgtcgtcg tgctcgtcgg cggccagctg gcgcaccagc
     1741 gcgtgcgtgc cgacgtcgta gtcccagagg gccatgcggt cgccaccatg gcgcgtgagc
     1801 accgcgaggt gacccttgtc ggcaagcact gccaccggca gcagcacctc gtcgttcacg
     1861 cgccgctcgt ccaccttgct ccaggctgcg gacgtgtcgg cgcggtacca gacggtgacg
     1921 cgggtattgt cggaccacag cgccgaatcc gtcgtctgca cggccagcag gtcgccacgt
     1981 cggtcggtga ccgcataggc gaggcgctgc cccgggatgt cgaactcgta gggaacgtgt
     2041 tcgagcgtgc gcacgtcgac ccgctcgatg tcgtgcatcg tgctcgcggt tgtcgatagt
     2101 gcccactcgg tcgggtggcc ggccgcgtcg tgcaggctcg agatgaagac cgcgcccggc
     2161 tcgagccgcc tggtctgccc gtccttcaag tcgaccacgt agccgcgggc accaccctga
     2221 tccacggtgt tgaagcccat caggtcgggc gcgaaccagc gcgcgcccga caaaggcatt
     2281 tccgtggcgg gcatcacgat cgtcgatttg tggccgaggg tatcgacgag caccgcctcg
     2341 cgttgggtgt ccagcgcgac gatggccagg atgtagcgac cgtccggcga gaggctcgcc
     2401 tcgacgatcg cgctggcctc caggaactgc tcgacaagtg ggtcgacccg tagcgccggc
     2461 ggtgcgtggt cttcggccag cacgggcatc gtgcaggcag cggccacggc gatcgccagg
     2521 ccgcgcagga tggtgcgcat cacgtggtct ccctgtgcgc gccggcgccc acgcggccgg
     2581 cgcgtcctct cgtcccgacg ccgcccggcg ccgtgttcct cgcccggcat tatcggcgcg
     2641 tctccgtcgc cgtcagcccg ccgcctcgat caccatctgc acccgctgct gcccctggaa
     2701 ctcgtccagg ctgaggcggt aggcgatgcg cacgcggtcg gcgacgggct cggcgtggcc
     2761 gaaccagatc gcgtcgcgca gcgtgccgcc gtggcgcacg cgcagcttca ggtgcttctc
     2821 gccgaccagc cgctgctgca cgacgtcgac ctcgtcgcag aaggtcgggg cctcgaagcc
     2881 ctggccccag acctgggcgt cgagtgcggc caccgtgacg gcgttgaacc actcgtgcgc
     2941 gagcgggccg tcggtggcca gcacgcgcgc gagctgcgcc gggccgagcc attcctcggc
     3001 gaccgcggcg aacgcgcggt cgaaggtggc gaagtcctcc cgcgcgagcg tcgcgcccgc
     3061 ggccatcgca tggccgccga acttgcgcag caggccgggg tggcgcttgc tgacgaggtc
     3121 gagcgcgtcg cgcaggtgga atccggcgat cgagcggccg gagcccttga gcaggccgtc
     3181 ggcgccgacg gcgaacacga aggtcggccg atgcaggcga tccttcacgc ggccggcgac
     3241 gatgcccacg accccttcgt ggaagtcctc gtggaacagc gcgagcgcgg ccggcgccgc
     3301 gccgtcgggc atcacgcgct cgacgagcgc ctcggcctgc tcgcgcatgc cggcctcgac
     3361 gtcgcggcgc tcgcggttga tgcggtcgag ctccgcggcc agcgcggcgg cgcgcgaggc
     3421 gtcgtccgtg agcaggcact cgatgcccag cgtcatgtcg gccaggcggc cggcggcgtt
     3481 caggcgcggg ccgagcgcga agcc
//
LOCUS       DASVLN010000623         4402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_193814, whole genome shotgun sequence.
ACCESSION   DASVLN010000623 DASVLN010000000
VERSION     DASVLN010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193814"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(741..3482)
                     /gene="glnE"
                     /locus_tag="VF453_14935"
     CDS             complement(741..3482)
                     /gene="glnE"
                     /locus_tag="VF453_14935"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEX7689008.1"
                     /translation="MAPSIGKALAEHSRYVQRVRRRYGDRLARLPQGLPDHATISALI
                     AGLESEGTPVMSALRIARHLVLERLAVLDIEQGADQAEVTAAMTHLAEATLERAHAQA
                     LTEVDPRWGVPRNEAGEPVEFWIVGMGKLGARELNVSSDIDLVYVYEEEAQTDGERPV
                     SAQEYFGQVARRMYALIGDVTEDGFVFRVDLALRPNGNSGPPVVSLAMLEEYFLVQGR
                     EWERFAWLKSRVVAPRAAVTSGRALALRSLVTPFVYRRYLDYGVFEGLRQLHHKIREE
                     AQRRAAGRPERANDVKLSRGGIREIEFIVQLLLVVRGGQFPEIRTRSTLKALARLTAA
                     GLMKPETATQLHEAYVFLRRVEHRIQFLDDQQTHLLPTADTDLGWISASLGLSCGKDA
                     CELLDRLGAIREFVSIEFDTLLHDGRSPPPRAGGCRGKMCSGAPPAQLDSESWLDRLP
                     AELAERVRRFADNPRVQALREESRLRIAKLVQRAVDEIRSGNCSIDAATRFVDWIEPL
                     LRRESYLALLVERPSVQRRLLRLLGLARWPMRYLMMHPGVIDELADERTMIERFDPAV
                     LTAELESRHEAWHRSGEADEGALLDTLRRAHHAEVFRTLVRDVEGRITVETVADELSS
                     LADTILDRSLRWAWHHLRQRHREEPRFAVIAYGKLGGKELGYGSDLDLVFLYDDAGEA
                     DPYRAQEAYGAFVRKLITWWTLRTAAGALFDIDTALRPNGNSGLLVTSIESFERYQRG
                     RGSNTAWTWEHQAITRARFCAGDAAIGERFEATRHAVIEAPRDPGALRVEIAAMRQKV
                     RDAHPQRDERIDPKHCEGGLMDIEFAVQYLVLAHGGAHAALRDNVGNIALLKRAEEAG
                     LLDEGIGTAAADAYRELRRAQHRARLDEQPTLFPPDTLAPERAAGRALWRAVFGPGA"
     gene            3663..>4402
                     /locus_tag="VF453_14940"
     CDS             3663..>4402
                     /locus_tag="VF453_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02099 family protein"
                     /protein_id="HEX7689009.1"
                     /translation="MTSQVSAFLEPVARRLRFMPWRRGLQLAWEAGVMLGLALSCLVV
                     LAWLTLQWGILPHIEDWRPAIERHASRAVGVPVRIGHIGVRSSGWVPAFELRDVVLRD
                     ASGAEALRLPRVSVALSPRSLLALEPRLAQLYVEGARLLVRRDMSGHLHVGGLDLAAR
                     PSVASADEDATLDWLFSQPELVLRHGAVRWVDEQRAAPALELSDVDIVLRNGLRSHDL
                     RVDATPPPEWGDRAGLRLRARQPLFARAG"
BASE COUNT          538 a         1540 c         1696 g          628 t
ORIGIN      
        1 ggtcggcgcc cggccggccc tcgagcgccg cgaacgcgtt cggatgcgaa ccgccgatcg
       61 cagtgaccag caccgcaccg acggccagcg cgctgcacag cacgagccag gcgacccccg
      121 gcgtgcgcag gccgcccgtc atgcaggcgc tccgtcgtca cgcgtccggg catgcgtcgc
      181 cgttcgcatg cccgcgcgcg cggggccgcc tgcgcgggcg ggcgggttcg gagtggacgg
      241 cgagggcagg ggcatggggc ggtcgcgatg cggcggagcc ggccggtcgc cgacgcgcgg
      301 attgtgcgtc cagctgggcg gtgcggcgcg cctgttactt ctggccaggc gtcgacacgg
      361 ggttgcgcgc ggtccgtccg gcgcgtgtgg ccgtcgatac ccacgcgggt accgcgtgga
      421 cccggcggcg gatgggcggc ggatgggcgg cggatgggcg gtggatgggc ggtggagcgg
      481 gcggtggagc gggcgcgaca tcggcggcgc gtccggcgga tcgcgccatg ccacgcgcgc
      541 gggcgacgcc gccctcgagc gcgagacgtc gtccgccgag cgcgccagga atcggcgacg
      601 ggaggtcgat gcgtcggggt ctggacggga ggttcgcgcg tcgtcgccgg acgagctcct
      661 ggccaccctc gatgccatgg tgcgcaccga tcccgcccgg gattccgagt tcgagagggc
      721 ttgcctcggg cctcggttcc tcaggcgccc ggcccgaaca cggcgcgcca cagcgcacgc
      781 ccggcggcgc gctcgggcgc gagcgtgtcg ggcgggaaca gcgtcggctg ctcgtcgagg
      841 cgtgcgcggt gctgggcgcg gcgcagctcg cgataggcgt cggcggcggc ggtgccgatg
      901 ccttcgtcga gcaggccggc ctcctcggcg cgcttgagca gggcgatgtt gccgacgttg
      961 tcgcgaagcg cggcatgggc gccgccgtgg gcgagcacga ggtactgcac ggcgaactcg
     1021 atgtccatca ggccgccttc gcagtgcttg ggatcgatgc gctcgtcgcg ctgcgggtgg
     1081 gcgtcgcgca ccttctgtcg catggcggcg atctcgacac gcagggcgcc cggatcgcgc
     1141 ggcgcttcga tgacggcgtg gcgcgtggcc tcgaagcgct cgccgatggc ggcgtcgccg
     1201 gcgcagaagc gggcgcgcgt gatcgcctgg tgctcccagg tccacgcggt gttgctgccg
     1261 cgtccgcgct ggtagcgctc gaaagactcg atcgaggtca cgagcagccc cgagttgccg
     1321 ttgggccgca gcgcggtgtc gatgtcgaac agcgcgcccg cggccgtgcg cagcgtccac
     1381 cacgtgatga gcttgcgcac gaaggcgccg taggcctcct gcgcgcggta cggatcggcc
     1441 tcgccggcgt cgtcgtagag gaagacgagg tcgaggtcgc tgccgtagcc cagctccttg
     1501 ccgcccagct tgccgtaggc gatgaccgcg aagcgcggct cctcgcgatg gcgctggcgc
     1561 aggtggtgcc aggcccagcg caggctgcgg tcgaggatgg tgtcggcgag gctggagagc
     1621 tcgtcggcga cggtctcgac cgtgatgcgg ccctcgacgt cgcgcaccag ggtgcggaag
     1681 acctcggcat ggtgggcgcg gcgcagcgtg tcgagcagcg cgccttcgtc ggcctctccg
     1741 gagcggtgcc aggcctcgtg gcggctctcg agctccgcgg tgagcacggc ggggtcgaag
     1801 cgctcgatca tcgtgcgctc gtcggcgagc tcgtcgatga cgccgggatg catcatcagg
     1861 tagcgcatcg gccagcgcgc cagccccagc aggcgcagca ggcggcgttg cacgctcggg
     1921 cgctcgacca gcagcgcgag gtagctctcg cggcgcagca gcggctcgat ccagtcgacg
     1981 aagcgcgtcg ccgcgtcgat ggagcagttg cccgagcgga tctcgtcgac cgcgcgctgc
     2041 accagcttgg cgatgcgcag ccggctctcc tcgcgcagcg cctgcacgcg cgggttgtcg
     2101 gcgaagcggc ggacgcgctc ggccagctcg gcgggcagac gatccagcca gctctccgag
     2161 tccagctgcg ccggcggcgc gccgctgcac atcttcccgc ggcagccgcc cgcgcgcggc
     2221 ggcggagagc ggccgtcgtg cagcagggtg tcgaactcga tcgagacgaa ctcgcggatc
     2281 gcgccgagcc ggtcgagcag ctcgcaggcg tccttgccgc acgacaaccc gaggctcgcc
     2341 gagatccagc cgaggtcggt gtcggccgtc ggaagcaggt gggtctgctg gtcgtcgagg
     2401 aattggatcc ggtgctcgac gcgacgcagg aagacatagg cttcgtgcag ctgcgtcgcc
     2461 gtctccggct tcatcagccc cgcggccgtg agcctggcga gcgccttcag cgtcgagcgc
     2521 gtgcggatct ccgggaactg gccgccgcgc acgacgagca gcagctgcac gatgaactcg
     2581 atctcgcgga tgccgccgcg cgagagcttc acgtcgttgg cgcgctccgg gcggccggcg
     2641 gcgcggcgct gcgcctcctc gcggatcttg tggtgcagct ggcgcaggcc ctcgaagacg
     2701 ccgtagtcga ggtagcgccg atacacgaag ggcgtgacga gcgagcgcag cgccagcgcg
     2761 cgcccgctcg tgaccgccgc ccgcggcgcg acgacgcggc tcttgagcca cgcgaagcgc
     2821 tcccactcgc ggccctggac gaggaaatac tcctccagca tcgccaggct caccaccggc
     2881 ggacccgagt tgccgttggg ccgcagcgcg aggtcgacgc ggaagacgaa gccgtcctcc
     2941 gtcacgtcgc cgatcagcgc gtacatgcgc cgggcgacct ggccgaagta ctcctgcgcg
     3001 ctcacgggcc gctcgccgtc ggtctgggcc tcttcctcgt agacgtagac caggtcgatg
     3061 tccgaggaca cgttgagctc gcgggcgccg agctttccca tgccgacgat ccagaactcg
     3121 accggctcgc cggcctcgtt gcgcggcacg ccccagcgcg ggtcgacctc cgtgagcgcc
     3181 tgcgcgtgcg cgcgctcgag cgtggcctcg gcgaggtggg tcatcgccgc ggtgacctcg
     3241 gcctggtcgg cgccctgctc gatgtcgagg acggccaggc gctccagcac gaggtggcgc
     3301 gcgatgcgca gcgcgctcat cacgggcgtg ccctcggact cgaggccggc gatgagcgcg
     3361 ctgatggtcg cgtggtcggg cagtccttgc ggcagccggg cgaggcgatc gccgtagcgc
     3421 cgccgcaccc gctgcacgta gcggctgtgc tcggccaggg ccttgccgat cgacggcgcc
     3481 atgggccgca gcaggcggtt gggcggcggg acgtcgttca tcgccgctct ttacgcgagg
     3541 acaggttcat gaaaagagcg gtggccgcag ggggaacaat cgcctcgtcc cgccgggcgt
     3601 ggcgcctgcc gtgcacacct acactgtggt ctggggtgcc gcgaccccgt gccgaactct
     3661 tcatgacatc gcaagtgagc gcattcctcg aaccagtcgc caggcggctg cgcttcatgc
     3721 cgtggagacg cggactccag ttggcctggg aggccggcgt gatgctcggc ctcgcgctgt
     3781 cgtgcctggt cgtccttgcc tggctgactc tgcagtgggg gattctgccg cacatcgagg
     3841 attggcgccc cgcgatcgag cggcacgcct cccgcgcggt aggggttccc gtgcgcatcg
     3901 ggcacatcgg cgtgcgctcg tcgggctggg tgccggcgtt cgagctgcgc gacgtcgtgc
     3961 tgcgcgacgc ctccggcgcg gaggcgctgc ggctgccgcg cgtgtcggtc gcgctgtcgc
     4021 cgcgctcgct gcttgcgctg gagccgcggc tggcgcagct ctacgtcgag ggcgcccggc
     4081 tgctggtgcg gcgcgatatg tccggccacc tgcacgtcgg cgggctggac ctcgcggcca
     4141 ggccgtccgt cgcgagcgcc gacgaggacg ccacgctcga ctggctcttc agccagcccg
     4201 agctcgtgct gcgccacggc gccgtgcgct gggtcgacga gcagcgggcc gcgcccgcgc
     4261 tcgagctgtc ggatgtcgac atcgtgctgc gcaacggcct gcgctcgcac gacctgcgcg
     4321 tcgacgccac gccgccgccg gagtggggcg accgcgcggg cctgcgcctg cgcgcgcgcc
     4381 agccgctgtt cgcgcgcgcc gg
//
LOCUS       DASVLN010000624         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_463972, whole genome shotgun sequence.
ACCESSION   DASVLN010000624 DASVLN010000000
VERSION     DASVLN010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463972"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF453_14945"
     CDS             <1..213
                     /locus_tag="VF453_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689010.1"
                     /translation="AVDRAAGELADDDAPGEPPAGEPAMPGHVESEARPPCAGRDDDG
                     AVDLDVDLDDGAGREVESRLAGELAH"
     gene            213..1535
                     /gene="sctN"
                     /locus_tag="VF453_14950"
     CDS             213..1535
                     /gene="sctN"
                     /locus_tag="VF453_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HEX7689011.1"
                     /translation="MDATFQRFVSALERELEQEPGIARTGRVFEVVGTMIKASGLEAR
                     VGELCELRHPRGAVIGRAEVVGFARSGAILSPFGSLSGVSEGTLVAGTGRPLSVPVGD
                     ALLGRVLSGLGDPIDGGGPIASNESRPALADPPDPMGRGMIERPLPTGVKVIDALMTL
                     GEGQRMGIFAPAGCGKSTLMGMLARGAQCDVSVIALIGERGREVREFIEQILGPEGMA
                     RSVVICATSDRSSIERAKAAYVATAVAEYFRDRGQRVLLMMDSLTRFARALREIGLAA
                     GEPPARRGFPPSVFAELPRLLERTGMGATGSITALYTVLAEDESGGDPIAEEVRGILD
                     GHMILSRKIAAKNQYPAIDVLGSLSRVMSQIVPREHAGAAGNLRMLMSKYDEIEMLMQ
                     VGEYRPGNDAVADIAAERIGEIREFLGQRTDELMAYEDILGQLAQVVR"
     gene            1602..>1805
                     /locus_tag="VF453_14955"
     CDS             1602..>1805
                     /locus_tag="VF453_14955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689012.1"
                     /translation="MSTRLKSLRTLVMVRERQAQALERELADARTRHEQACGRLQDAT
                     GHERACLAGEALAVQDRRALTSGA"
BASE COUNT          243 a          649 c          687 g          226 t
ORIGIN      
        1 gccgtcgacc gcgccgccgg cgagctcgcg gacgacgacg cgccgggcga accgcccgcc
       61 ggcgaaccgg ccatgcccgg gcacgtggaa tccgaggcgc gcccgccgtg cgccgggcgg
      121 gacgacgatg gcgcggtcga cctcgacgtc gacctcgatg acggtgccgg ccgcgaggtc
      181 gagtctcgcc tggccggcga gctcgcgcac tgatggacgc gacgttccag cgcttcgtct
      241 cggcgctcga gcgcgagctc gaacaggagc cgggcatcgc gcgcaccggg cgcgtgttcg
      301 aggtcgtcgg cacgatgatc aaggccagcg gcctcgaggc gcgcgtgggc gagctgtgcg
      361 agctgcgcca tccgcgcggc gccgtgatcg ggcgcgcgga ggtcgtcggc ttcgcacgca
      421 gcggcgccat cctgtcgccg ttcggctccc tgagcggcgt gagcgagggc acgctggtgg
      481 ccggcaccgg ccgcccgctg tcggtgcccg tgggcgacgc cctgctcggg cgcgtgctga
      541 gcggcctggg cgacccgatc gacggcggcg gcccgatcgc gtccaacgag tcccgcccgg
      601 cgctggccga tccgcccgac ccgatgggcc gcggcatgat cgagcgcccg ctgcccaccg
      661 gcgtcaaggt gatcgacgcg ctgatgacgc tcggcgaggg ccagcgcatg ggcatcttcg
      721 cgcccgcggg ttgcggcaag agcacgctga tgggcatgct ggcgcgcggc gcgcaatgcg
      781 acgtcagcgt catcgcgctg atcggcgagc ggggacgcga ggtgcgcgag ttcatcgagc
      841 agatcctggg ccccgagggc atggcgcgtt cggtcgtgat ctgcgcgacg tccgaccgct
      901 ccagcatcga gcgcgccaag gcggcctacg tcgcgaccgc ggtggccgaa tacttccgcg
      961 accgcggaca gcgcgtgctg ctgatgatgg actcgctgac ccgcttcgcg cgcgcgctgc
     1021 gcgagatcgg cctggcggcg ggggagcctc cggcgcgccg gggcttcccg ccctcggtct
     1081 tcgccgagct gccgcgcctg ctcgagcgca ccggcatggg ggcgaccggc tcgatcaccg
     1141 cgctctacac cgtgctcgcc gaggacgaga gcggcggcga tccgatcgcc gaggaagtgc
     1201 ggggcatcct cgacggccac atgatcctgt cgcgcaagat cgcggcgaag aaccagtacc
     1261 ccgccatcga cgtgctgggc agcctgtcgc gcgtgatgtc gcagatcgtg ccgcgcgagc
     1321 atgccggcgc ggccggcaac ctgcggatgc tgatgtccaa gtacgacgag atcgagatgc
     1381 tcatgcaggt gggcgagtac cgccccggca acgacgccgt ggcggacatc gccgcggagc
     1441 ggatcggcga gatccgcgaa ttcctcggcc agcgcaccga cgagctgatg gcctacgagg
     1501 acatcctcgg ccagctggcc caggtggtgc gctgacatgt tcgcccgccc ggccgctcgg
     1561 acgaggcctc gcgctccggc ccgcacgacg gagtccggat cgtgagcacg cgcctgaagt
     1621 cgctgcgcac gctcgtgatg gtccgggagc gccaggcgca ggcgctcgag cgcgagctcg
     1681 ccgacgcgcg cacccgccac gagcaggcct gcggccgcct gcaggacgcg accgggcacg
     1741 agcgggcctg cctcgccggc gaggcgctgg cggtgcagga tcgacgcgcg ctcacgagcg
     1801 gcgcg
//
LOCUS       DASVLN010000625         5475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_142476, whole genome shotgun sequence.
ACCESSION   DASVLN010000625 DASVLN010000000
VERSION     DASVLN010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142476"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VF453_14960"
     CDS             complement(<1..438)
                     /locus_tag="VF453_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689013.1"
                     /translation="MVARGRALNVMAAAAMLVLSTGASALGLGRVNVQSVLGEALRAE
                     IEVTSLNAEESSSLHVSLAPPDAFKASGVEYNPVLQGATITLSRRADGRPVLVLQSDK
                     VVQEPFVDVIVDFQWATGRLTRSYTLLIDPPGRNTSAQPGSTAV"
     gene            complement(903..2030)
                     /gene="asd"
                     /locus_tag="VF453_14965"
     CDS             complement(903..2030)
                     /gene="asd"
                     /locus_tag="VF453_14965"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX7689014.1"
                     /translation="MALVGLVGWRGMVGSVLMDRMQAERDFDLIEPLFFSTSAAGGAA
                     PAQAKNETKLQDAFDVEALKRCDIVITCQGGDYTNEVFPKLRAAGWNGHWIDAASSLR
                     MKDDAVIVLDPVNMPVIKNALARGQRDWIGGNCTVSCMLMGVGALYKAGLVEWMSTQT
                     YQAASGGGAQHMRELLTQYGTLNAEVKALLDDPKSAILEIDRRVIAKQRSLSAQETAN
                     FGVPLGGSLIPWIDKDLGNGTSKEEWKGMAETNKILGQGEGFGTPAVPVDGFCVRVGA
                     MRCHSQALTFKLKKDVPLADIEAMIAADNEWVKVVPNTREATLRDLTPVAVTGTMTIP
                     VGRLRKLAMGPEYLGAFTIGDQLLWGAAEPLRRMLRILLEK"
     gene            complement(2182..3294)
                     /gene="leuB"
                     /locus_tag="VF453_14970"
     CDS             complement(2182..3294)
                     /gene="leuB"
                     /locus_tag="VF453_14970"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX7689015.1"
                     /translation="MKIAILPGDGIGTEIVAEAVKVLGALDLRFELEHADVGGAAYER
                     HGHPLPEATLALAKAADAVLFGAVGDWKYDKLERALRPEQAILGLRKHLQLFANLRPA
                     ICHAPLTHASSLKPELVAGLDILIIRELTGDIYFGQPRGRRTAVDGHFPGAEEAFDTM
                     RYSRPEIERIAHVAFQAARKRGKRVTSVDKANVLETFQFWKDVVTEVHAQYPDVELDH
                     MYVDNAAMQLVKAPRKFDVVVTGNMFGDILSDEAAMLTGSIGMLPSASLDANNKGLYE
                     PSHGSAPDIAGKGIANPLATILSAAMMLRYTLAQPEAADRIESAVGSVLASGLRTGDI
                     ASAGTRTVGTREMGDAVVAALRAQATGTTTITKTTS"
     gene            complement(3334..3984)
                     /gene="leuD"
                     /locus_tag="VF453_14975"
     CDS             complement(3334..3984)
                     /gene="leuD"
                     /locus_tag="VF453_14975"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7689016.1"
                     /translation="MQAFTIHTGLVAPMDRENVDTDAIIPKQFLKSIKRTGFGPNLFD
                     EWRYKDPGYPGQDPATRVPNPEFVLNQPRYQGASVLIARRNFGCGSSREHAPWALDQF
                     GFRAVIAPSFADIFFTNCFKSGLLPIVLPEDAVANLFDDVNAFVGYKLTIDLPRQVVA
                     KPDGAEIAFDVQPFRKYCLVNGFDDIGLTLRHADKIRAFEGERLARMPWLANRLVG"
     gene            complement(4001..5413)
                     /gene="leuC"
                     /locus_tag="VF453_14980"
     CDS             complement(4001..5413)
                     /gene="leuC"
                     /locus_tag="VF453_14980"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX7689017.1"
                     /translation="MTGRTLYDKLWDDHVLHAEEDGTALLYIDRHLLHEVTSPQAFEG
                     LDIAGRKVWRLSANLAVSDHNVPTTDRRGGIADPVSKLQVDTLDANCDRAGITQFKMT
                     DKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLL
                     AKKAKNLLVRVEGTLARGCGAKDIVLAIIGRIGTAGGTGYTIEFAGAAIRALSMEGRM
                     TVCNMAIEAGARAGLVAVDDTTIAYVKGRPFSPVGVEWDQAVAYWRTLHSDADAHWDR
                     VVEIDASQIRPQVTWGTSPEMVLSIDDRVPDPEREKDEVKRGAMERALTYMALEPNKA
                     IADITIDKVFIGSCTNSRIEDLREAAAVVRRVGGRIAGNVRLALVVPGSGLVKEQAER
                     EGLDQVFLAAGFEWREPGCSMCLAMNADRLDPGERCASTSNRNFEGRQGAGGRTHLVS
                     PAMAAAAALHGHFVDARKFS"
BASE COUNT          793 a         1865 c         1904 g          913 t
ORIGIN      
        1 gaccgcggtc gagcccggct gcgccgaggt gttgcggccc gggggatcga tcagcagcgt
       61 gtacgaacgc gtcagccggc cggtcgccca ctggaagtcg acgatgacgt cgacgaaagg
      121 ctcctgcacg accttgtcgc tctgcaggac cagcacggga cggccatcgg cgcggcgcga
      181 cagcgtgatc gtcgcgccct gcagcacggg gttgtattcg acgcccgagg ccttgaaggc
      241 gtcgggcggc gcaaggctca cgtgcagcga cgacgattct tcggcgttca ggctcgtgac
      301 ctcgatctcg gcgcgcaacg cttcgccgag cacggattgc acgttcacgc gccccagccc
      361 cagtgccgaa gcaccggtcg acaggacgag catggccgcg gcggccatga cgttcagcgc
      421 gcgccctctg gcgaccaacg agcgattctt caattcgtct cccgaacgca aattggcgat
      481 cccgtgggat cgccctggat gaccgaccca gaccgggaca tcggaaaaag acgcgcccag
      541 taaaggcctg cgcgcatcag ggaaaagacc atagcatcgc ggggatacga agacaagctc
      601 aagcaaagag tcaagtcccg gccgccccgg cggctccaga cacgaaagcc agcccgaata
      661 ttctcgcact gccgcggcgg gtttagcccg acgcaggcgc gcattttgcc gcttcgggca
      721 cccccgcgaa ggggagaatt accccgaaat aatcgaaaac gccacgttgg aggcgatttc
      781 cggcccggta gagcggcagg gaagtaacaa gatatttcgc cggtgacctg ccgtgcaggg
      841 tttgcggcag cgcacggcgc ccgccctccc ggacgagcgc cgcgcggcga cggcgtggtc
      901 gattacttct cgagcagaat ccgcagcatg cggcgcagcg gctccgcggc gccccacagc
      961 aattggtcgc cgatcgtgaa tgcgccgaga tactccgggc ccatcgcgag tttgcgcagg
     1021 cggcccaccg gaatcgtcat cgtgcccgtg accgccaccg gcgtcaggtc ccgcagcgtc
     1081 gcctcgcgag tattcggcac gaccttcacc cactcgttgt cggcggcgat catcgcctcg
     1141 atgtccgcga gcggcacgtc cttcttcagc ttgaaggtca gcgcctggct gtggcagcgc
     1201 atcgcgccga cgcgcacgca gaagccgtcg accggcaccg ccggcgtgcc gaagccctcg
     1261 ccctggccga ggatcttgtt ggtctcggcc atgcccttcc attcttcctt ggaagtgccg
     1321 ttgcccaggt ccttgtcgat ccagggaatc agcgagccgc cgagcggcac gccgaagttg
     1381 gccgtctcct gcgcggagag cgagcgctgc ttcgcgatga cgcggcgatc gatctccagg
     1441 atcgcgctct tcggatcgtc gagcagcgcc ttgacctcgg cgttgagggt gccgtactgc
     1501 gtcagcagct cgcgcatgtg ctgcgcaccg ccgcccgagg ccgcctggta ggtctgggtg
     1561 ctcatccact ccaccaggcc cgccttgtac agcgcgccca cgcccatcag catgcagctg
     1621 acggtgcagt tgccgccgat ccagtcgcgc tggcctctgg cgagcgcgtt cttgatgacg
     1681 ggcatgttga ccgggtcgag cacgatgacg gcgtcgtcct tcatgcgcag cgacgaggcg
     1741 gcgtcgatcc agtggccgtt ccagcccgcg gcgcgcagct tggggaagac ctcgttcgtg
     1801 tagtcgccgc cctggcaggt gatcacgatg tcgcagcgct tgagcgcctc gacgtcgaac
     1861 gcgtcctgca gcttcgtctc gttcttcgcc tgcgccgggg ccgcgccgcc ggccgcgctg
     1921 gtggagaaga acagcggctc gatcaggtcg aagtcgcgct ccgcctgcat ccggtccatg
     1981 agcacggagc cgaccatgcc ccgccagcct acgagtccta ccaatgccat ttcgtttcgc
     2041 cttgttgcgc gtccttcgcg cctctggact gtcactcgtt gacgaccctc gcgggccgcc
     2101 gcctgtcctg cttcccgccc ctcgactcgc gtcgccgggg atctgtcttt cagggaagca
     2161 ggggcgaaac gaggcggagc gtcagctcgt ggttttggta atcgtggtgg tgccggttgc
     2221 ctgcgcgcgc agggccgcga cgacggcatc gcccatctcg cgcgtgccca ccgtgcgcgt
     2281 gcccgcggac gcgatgtccc cggtgcgcag cccgctcgcc agcaccgagc ccaccgccga
     2341 ctcgatgcgg tcggcggctt ccggttgggc gagggtgtag cggagcatca tggcagcaga
     2401 cagtattgta gccagaggat tcgcgatccc cttgcccgcg atgtccggcg cactgccgtg
     2461 gctgggctcg tacaggccct tgttgttcgc gtccaacgag gccgagggca gcatgccgat
     2521 cgagccggtc agcatcgccg cctcgtcgga caggatgtcg ccgaacatgt tgccggtgac
     2581 gacgacgtcg aacttcctgg gcgctttcac gagctgcatc gcggcgttgt cgacgtacat
     2641 gtggtcgagc tcgacgtccg ggtactgcgc gtgcacctcg gtcacgacgt ccttccagaa
     2701 ctggaaggtc tcgagcacgt tggccttgtc gacgctcgtc acgcgcttgc cgcgcttgcg
     2761 ggcggcctgg aaggcgacgt gcgcgatgcg ctcgatctcc ggacggctgt agcgcatcgt
     2821 gtcgaaggcc tcctcggccc ccgggaagtg gccgtcgacg gccgtgcgcc ggccgcgcgg
     2881 ctggccgaag tagatgtcgc cggtgagctc gcggatgatc aggatgtcca gcccggccac
     2941 cagctccggc ttgaggctgg acgcgtgggt cagcggcgca tggcagatcg ccgggcgcag
     3001 gttcgcgaac agctgcaggt gcttgcgcag gccgaggatc gcctgctccg gacgcagcgc
     3061 gcgctcgagc ttgtcgtact tccagtcgcc gacggcgccg aagagcaccg cgtcggccgc
     3121 cttggccagc gcgagcgtgg cctcgggcag cggatggccg tggcgctcgt aggccgcgcc
     3181 gccgacgtcg gcgtgctcga gctcgaagcg caggtccagc gcgccgagca ccttgacggc
     3241 ctccgcgacg atctcggtgc cgatgccgtc gcccggcagg atggcgatct tcatgatgcg
     3301 tgctttctcg tgatacgtca gtggggtatc ggatcagccg accagtcgat tggccagcca
     3361 cggcatgcgc gccagccgct ccccctcgaa ggcgcggatc ttgtcggcgt ggcgcagcgt
     3421 caggccgatg tcgtcgaagc cgttgacgag gcagtacttg cggaacggct ggacgtcgaa
     3481 cgcgatctcg gcgccgtcgg gcttggcgac gacctggcgc ggcaggtcga tggtcagctt
     3541 gtagccgacg aaggcgttca cgtcgtcgaa caggttcgcg accgcgtcct ccggcagcac
     3601 gatgggcagc aggccgctct tgaagcagtt ggtgaagaag atgtcggcga agctcggcgc
     3661 gatgaccgcg cggaagccga actggtcgag cgcccacggc gcgtgctcgc ggctcgagcc
     3721 gcagccgaag ttgcggcgcg cgatcagcac cgacgcgccc tggtagcgcg gctggttcag
     3781 cacgaactcg gggttgggca cgcgcgtggc cggatcctgg cccgggtagc cgggatcctt
     3841 gtagcgccac tcgtcgaaca ggttgggccc gaagccggtg cgcttgatcg acttcaggaa
     3901 ctgcttgggg atgatcgcgt cggtgtcgac gttctcgcga tccatcgggg cgacgaggcc
     3961 ggtgtggatg gtgaaggctt gcatggcggt ctctccccgg tcagctgaat ttgcgggcgt
     4021 cgacgaagtg gccatgcagc gcggccgcgg cggccatcgc cgggctcacg aggtgcgtgc
     4081 ggccgccggc gccctgccgg ccctcgaagt tgcggttgct ggtcgacgcg cagcgctcgc
     4141 ccgggtcgag gcggtcggcg ttcatcgcca ggcacatcga gcagcccggc tcgcgccact
     4201 cgaagccggc ggcgaggaag acctggtcga ggccctcgcg ctcggcctgc tccttcacga
     4261 ggcccgagcc cggcaccacc agcgcgagcc gcacgttgcc ggcgatgcgc ccgcccacgc
     4321 gccgcacgac ggccgcggcc tcgcgcaggt cctcgatgcg gctgttggtg cacgagccga
     4381 tgaagacctt gtcgatcgtg atgtcggcga tggccttgtt cggctccagc gccatgtagg
     4441 tgagcgcgcg ctccatcgcg ccgcgcttga cctcgtcctt ctcccgctcg ggatcgggca
     4501 cgcggtcatc gatggacagc accatctccg gcgacgtgcc ccaggtgacc tgcgggcgga
     4561 tctgcgacgc gtcgatctcc acgacgcgat cccagtgcgc gtcggcgtcc gagtgcagcg
     4621 tgcgccagta ggccacggcc tggtcccact cgacgcccac cggcgagaac ggccggccct
     4681 tcacgtaggc gatggtcgtg tcgtcgacgg ccaccaggcc cgcgcgggcg ccggcctcga
     4741 tggccatgtt gcagaccgtc atgcggcctt ccatcgacag cgcccggatc gcggcgcccg
     4801 cgaactcgat cgtgtagccg gtgccgccgg ctgtgccgat gcggccgatg atcgccagca
     4861 cgatgtcctt ggcgccgcag ccgcgggcga gcgtgccctc cacgcgcacg agcaggttct
     4921 tcgccttctt ggcgagcagc gtctgcgtgg cgagcacgtg ctcgacctcg ctggtgccga
     4981 tgccgtgcgc cagcgcgccg aacgcgccgt gcgtcgaggt gtggctgtcg ccgcagacca
     5041 ccgtcatgcc gggcagcgtg gcgccctgct ccggcccgat cacgtgcacg atgccctggc
     5101 gcttgtccgt catcttgaac tgcgtgattc cggcgcggtc gcagttggcg tcgagcgtgt
     5161 cgacctgcag cttggagacc gggtccgcga tgccgccgcg gcggtcggtg gtgggcacgt
     5221 tgtggtcgct cacggcgagg ttggcggaca ggcgccagac cttgcggccg gcgatgtcca
     5281 gcccctcgaa ggcctgcggg ctcgtgacct cgtgcagcag gtggcggtcg atgtacagca
     5341 gggcggtgcc gtcttcctcg gcgtgcagga cgtgatcgtc ccagagcttg tcgtacagag
     5401 tgcgtccggt catggcgtca ccttttcgtt ggcggggagt ccgcggccgc gcggcgcggc
     5461 gggcgcggca tcggc
//
LOCUS       DASVLN010000626        10302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_32686, whole genome shotgun sequence.
ACCESSION   DASVLN010000626 DASVLN010000000
VERSION     DASVLN010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10302
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32686"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VF453_14985"
     CDS             complement(<1..389)
                     /locus_tag="VF453_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689018.1"
                     /translation="MIRRSIAAAGALALLALAGCASGPAGAMLTYETSPLGATIYEGG
                     QAVGVAPVTRTYAGDGKSTEVRTPEVTAVWPSGAKATFWTILHVGDDRVTTIQRPANA
                     PGLDVDKAAAAKIADENARADARAKDAA"
     gene            complement(452..1969)
                     /locus_tag="VF453_14990"
     CDS             complement(452..1969)
                     /locus_tag="VF453_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEX7689019.1"
                     /translation="MENGPLDIVIVGGGTSGWMTAAALSKVLKGRYRITLVESDEIGT
                     VGVGESTIPMISLFNRMLEIDEDEFMRETQASFKLGVEFVNWGRIGDRYIHGFGVIGQ
                     DNWTVDFHQYWLKQYQAGKAKALEHYSINTAACLQGKFMRPRYDMPKSPLSTIPYAFH
                     FDASLYARYLRRYAEARGVVRVEGRITDVALREGDGHVESVTLQSGRRIEGGLFIDCS
                     GFRALLIEGALKTDFEDWSHWLPCDRAVAVPCTPVAELTPYTRAIARPAGWQWRIALQ
                     HRIGNGHVYSSAHMSDDEAIGVLLANLDGAPLAEPRPLRFKAGRRKQAWNRNVVSIGL
                     ASGFLEPLESTSIHLVQMGIAHLLTYFPSAGFDAADIDLYNRTIQQEYEWVRDFIILH
                     FKATERTDTPFWNQCREMSVPDTLQERIDLFRAHGRVLRKGDELFAKPSWVQVMHGQR
                     IRPRSYHPLADLIDEPEIQKYLEEVEGVIAACVRVMPTHNDFIARHCAAPMPKAA"
     gene            complement(2075..4921)
                     /locus_tag="VF453_14995"
     CDS             complement(2075..4921)
                     /locus_tag="VF453_14995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7689020.1"
                     /translation="MKNSSATRAGSALFKLKPTMVLAGQATAGALALAASGTVFAQGA
                     AAAPQPAASEPTVGQSVTVVGIRKGIEAAISVKKNSDQIVEAISAEDIGKLPDTTIAE
                     SLAHLPGVTSQRDSTGKATNVNIRGLSSDFNGYLLNGREQTSTTDSRGVDLSVYPAEL
                     IAGATVYKTSDASLATAGLAGTIDNKLVDPLAFPNRVVAVSGQKGHPSVGLKPHGNET
                     RESITYIDQFADRKIGVALGFVRVDGSSSQLGVSSWATDPKKPLQAHPVGDDSATIAG
                     ATLPFSGGMGFDSANISDKRNGLAAILEFKPNDRFRTELDIFHAQINTDTHHTTLKAP
                     LPIDVYDATVDPTTNQVTSGTYRLGGNPNGIIDYTENVFDDDTLQSMGWRSEFKFNDA
                     WRASLDVNHNTARRIERDIEAYAGIPTADNVSFTLPVGGQVPHLTFGTPTSYTDAKTI
                     VVRDQSGWSGITGVPQDGYDKGPTTVDKITAVRADITHDLPENRWFSDVQFGANLSQR
                     TKNRTAVEGLVVADTPDGTGTIPFPAGSYVVNDVGGTGASLLTFSPTADLWPGAKLQS
                     KYNNDILSKTWNITEDVSTAYGKLDIDTEMAKVPVRGNVGLQIVHTRQQSQGFQANAA
                     ADPTLTNPAAGLSTAGTSYTDYLPSLNLVGDLGNGNTLRFGASVQTARPDMTFLRNSS
                     SVGFNNTDGREEASGGNAYLKPYKAKALDLSYEKYFANKAYLAAAVFYKRLDTYVAQQ
                     TTITDLQGAANAVGQTALPAGYSSWVGPSTNYVNGHGGNVKGIELSASMPFNLLTRWL
                     DGFGTALSYSNTTSSIRQPDTIGLNPTQPIPQTGTISMPGLSHINEKIELYYEKAGFS
                     AFISDSYRSFYIGPVTNATVGGYPTLIGIGAQKWISAQVGYEFQSGWLKGLGFRLEGN
                     NLNKPKYTEYKGDGTVNTQKETGASIDLRVSYRL"
     gene            5322..7211
                     /locus_tag="VF453_15000"
     CDS             5322..7211
                     /locus_tag="VF453_15000"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX7689021.1"
                     /translation="MNAESTPDVKDSPWSPAAAANAEPRLLADIGATYARFCVEESPG
                     HFDHVVVLPCADFAGFADVVQAYLRGLEGTPPRHAAIAMANPIAGDRVQMTNRDWSFS
                     IEQVRQQLGFATFLVVNNFTALAMALPGLDGTQRVQVGGGEAQGDGVIALLGPGTGLG
                     VSGLIPADDRWSTLATEGGHVSFAPNDERELRVLQHAWKHLPHVSAERLVSGPGIELI
                     HEALTQGTAQAGQRLTTAEIVRRAVTRADPACAETVDCFCGMLGTVASDLALTLGATG
                     GVYIGGGIVPRLGELFERSSFRTRFESKGRFSAWLAKIPTFVLTADNPTFRGLSLLLA
                     EHVPDTSSPHSPLLDRVRASRDTLTPAERRVADVVLGNPRSLLNDAIAVIAQQAEVSQ
                     PTVIRFCRSLGFEGLADFKLKLASGLTGTIPVLRSQVRRGEPTADLSAKVLDNTISAI
                     VHFRESLNAAAIDRAIALLRGARRVEIFACGNSAVVALDAQHKLLRFRIPVVAQTDTS
                     LQSLAAEMLSPGDVAVFISSSGQTAELVRAAGVAIERGASVVAITASQSRLARLATVC
                     LGLDHAEDSSTFLSMVARILQLLVIDIMSVGLAVQRDTSSTREPGDGASRTTPAPGVR
                     ISHLS"
     gene            complement(7300..9570)
                     /locus_tag="VF453_15005"
     CDS             complement(7300..9570)
                     /locus_tag="VF453_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HEX7689022.1"
                     /translation="MLRFRFPIVIIDEDFRSENTSGLGIRALASAIEKEGIEILGVTS
                     YGDLSQFAQQQSRASAFILSIDDEEFTPGPELDPAVLNLRKFIQEIRSKNPEIPIYLY
                     GETRTSQHIPNDILRELHGFIHMFEDTPEFVARHIIREANSYLDGVAPPFFKALMDYA
                     ADGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNSVEELGQLLDHTGPVA
                     DSERNAARIFNADHCFFVTNGTSTSNKMVWHHTVAPGDVVVVDRNCHKSILHSIIMCG
                     AVPVFLTPTRNHYGIIGPIPQSEFTREAIERKIAANPLLEGVDPKAVKPRIMTLTQST
                     YDGVVYNTETIKSLLDGWIDTLHFDEAWLPHAAFHDFYSVYHAMGKARARPKEAMVYA
                     TQSTHKLLAGLSQASQVLVQDSQTRKLDRHLFNEAYLMHTSTSPQYAIIASCDVAAAM
                     MEPPGGNALVEESLKEALDFRRAMRKIEQEFGSGDWWFKVWGPEKFAEEGIGEAADWV
                     LEPDDESWHGFGNLAPGFNMLDPIKSTIITPGLDVSGNFSKDGIPASIVTKYLAEHGI
                     VVEKTGLYSFFIMFTIGITKGRWNTLLATLQQFKDDYDKNAPLWRILPEFCNAHRRYE
                     RMGLRDLCQAIHEVYTEGDIARLTTDMYLSDLHPAMKPSEAYARIAHRDTERVAIDDL
                     EGRITTSLLTPYPPGIPLLIPGERFNRKILAYLQFARAFNERFPGFDTDVHGLVIEED
                     SFGRKHYYVDCVRDAK"
     gene            9845..9935
                     /locus_tag="VF453_15010"
     tRNA            9845..9935
                     /locus_tag="VF453_15010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9879..9881,aa:Ser,seq:gga)
BASE COUNT         1512 a         3428 c         3578 g         1784 t
ORIGIN      
        1 gccgcgtcct tcgcgcgcgc gtcggcgcgg gcgttctcgt cggcgatctt cgcggccgcc
       61 gccttgtcga cgtccagccc gggcgcgttc gcgggacgct ggatcgtggt gacgcggtcg
      121 tcgcccacgt gcaggatggt ccagaacgtc gccttcgcgc cgctcggcca cacggccgtc
      181 acctcgggcg tgcgcacctc ggtggacttg ccgtcgccgg cataggtgcg cgtgaccggc
      241 gcgacgccga cggcctggcc gccctcgtag atcgtcgcgc ccagcggcga ggtctcgtaa
      301 gtcagcatcg cgcccgcggg ccccgatgcg cagcccgcca gcgcgagcag ggccagcgcg
      361 ccggcggcgg cgatggaacg gcggatcatg gaagtctcct tgtcttgtcg tcgtcggcgc
      421 ggccctcctc gagccgcgcg cggggaaggc gtcacgccgc cttcggcatc ggcgcggcgc
      481 agtggcgggc gatgaagtcg ttgtgcgtgg gcatgacgcg cacgcacgcg gcgatcacgc
      541 cttcgacttc ctcgaggtac ttctggatct cgggctcgtc gatgagatcc gccagcgggt
      601 ggtaggaccg cggccggatg cgctggccgt gcatcacctg cacccagctc ggcttggcga
      661 acagctcgtc gcccttgcgc agcacgcggc cgtgggcgcg gaacaggtcg atgcgctcct
      721 ggagagtgtc ggggacgctc atctcgcggc actggttcca gaacggcgtg tcggtgcgct
      781 ccgtggcctt gaagtgcagg atgatgaagt cccgcaccca ctcgtactcc tgctggatgg
      841 tgcggttgta cagatcgatg tcggcggcat cgaagccggc cgacgggaag taggtcagca
      901 ggtgggcgat gcccatctgc accaggtgga tgctggtgga ttcgagcggc tcgaggaacc
      961 ccgaggccag gccaatggag accacgttgc ggttccacgc ctgcttgcgc cggcccgcct
     1021 tgaagcgcag cgggcgcggc tcggccagcg gcgcgccgtc caggttggcg agcagcacgc
     1081 cgatcgcctc gtcgtcgctc atgtgggcgc tggagtagac atggccgttg ccgatgcggt
     1141 gctgcagcgc gatgcgccac tgccagcccg ccgggcgcgc gatcgcgcgc gtgtagggcg
     1201 tgagttcggc caccggcgtg cagggcaccg ccaccgcgcg gtcgcacggc agccagtgcg
     1261 accagtcctc gaagtccgtc ttcagcgcgc cctcgatcag cagcgcgcgg aagcccgagc
     1321 agtcgatgaa caggccgccc tcgatcctgc ggcccgattg cagggtgacc gactcgacgt
     1381 ggccgtcgcc ctcgcgcagc gcgacgtcgg tgatgcggcc ttccacgcgc accacgccgc
     1441 gcgcctcggc ataccgcctc aggtatcgcg catacaggct cgcgtcgaag tggaacgcgt
     1501 acgggatcgt cgacagcggc gacttgggca tgtcgtagcg cgggcgcatg aacttgccct
     1561 gcaggcaggc cgccgtgttg atcgagtagt gttccagcgc cttcgccttg cccgcctggt
     1621 actgcttgag ccagtactgg tggaagtcga ccgtccagtt gtcctggccg atgacgccga
     1681 agccgtggat gtagcggtcg ccgatgcggc cccagttgac gaactcgacg ccgagcttga
     1741 acgaggcctg cgtctcgcgc atgaactcgt cctcgtcgat ctcgagcatc cggttgaaca
     1801 gcgagatcat cgggatcgtc gactcgccca cgcccacggt gccgatctcg tcggactcca
     1861 ccagcgtgat gcggtacctg cccttgagca ccttggacaa cgccgctgcc gtcatccagc
     1921 cggacgtacc gccgcccacg atcacgatgt cgaggggacc gttctccatc atggcttcac
     1981 cttccgtcga aacgagaaga cccttgcgtg acgcgtcgcg caagggtcga ggatcggacg
     2041 ccgccggcgc ccgactttac gcctcaagcg ccgatcacag gcgataggac acgcgcaggt
     2101 cgatcgacgc gcccgtctcc ttctgcgtgt tgacggtgcc gtcgcccttg tactccgtgt
     2161 acttcggctt gttcaggttg ttgccctcga ggcggaagcc caggcccttg agccagccgg
     2221 actggaactc gtagccgacc tgcgccgaga tccacttctg cgccccgatg ccgatcagcg
     2281 tcgggtagcc gccgacggtc gcgttggtga ccggcccgat gtagaaggag cggtagctgt
     2341 cggagatgaa cgcgctgaac cccgccttct cgtagtacag ctcgatcttc tcgttgatgt
     2401 gcgacaggcc cggcatgctg atcgtgccgg tctgcgggat cggttgcgtc gggttcaggc
     2461 cgatggtgtc gggttggcgg atggagctgg tcgtgttgga gtagctcagg gcggtgccga
     2521 agccgtcgag ccagcgcgtc agcaggttga acggcatgga cgcggacagc tcgatgccct
     2581 tcacgttgcc gccgtgcccg ttgacgtagt tggtcgacgg cccgacccag ctggagtagc
     2641 ccgccggcag cgcagtctgg ccgaccgcgt tggccgcccc ctgcaggtcg gtgatcgtgg
     2701 tctgctgagc gacgtaggtg tccagccgct tgtagaacac cgcagctgcc agataggcct
     2761 tgttggcgaa gtacttctcg taggagaggt ccagcgcctt ggccttgtac ggcttgaggt
     2821 aggcgttgcc tcccgaagcc tcttcgcgtc cgtcggtgtt gttgaagccg accgaggacg
     2881 aattgcgcag gaaggtcatg tccggccgcg cggtctggac gctcgcgccg aagcgcagcg
     2941 tgttgccgtt gccgaggtca cccaccaggt tcaacgaagg caggtagtcg gtgtagctgg
     3001 tgccagccgt ggacaggccc gcggccgggt tcgtcagcgt cgggtcggcc gccgcattgg
     3061 cctggaagcc ttgcgactgc tgacgcgtgt ggacgatctg caggccgacg ttgccgcgca
     3121 ccggcacctt cgccatctcg gtgtcgatgt cgagcttgcc gtaggccgtc gacacatcct
     3181 cggtgatgtt ccaggtcttg gagaggatgt cgttgttgta cttggactgc agcttcgcgc
     3241 ctggccacag gtcggcggtc gggctgaagg tgagcaggct cgcgccggtg ccgccgacgt
     3301 cgttgacgac gtaggatccc gccgggaacg ggatcgtacc ggtaccgtcg ggggtatctg
     3361 caacgacaag accttccact gccgtgcgat tcttcgtgcg ctggctgaga ttggcgccga
     3421 actgcacgtc gctgaaccag cggttctcgg gcaggtcatg cgtgatgtcc gcgcgaaccg
     3481 ccgtgatctt gtcgaccgtg gtcgggccct tgtcgtagcc gtcctgcggc acgccggtga
     3541 tgccgctcca gcccgactgg tcgcggacca cgatggtctt ggcgtcggtg tagctggtcg
     3601 gcgttccgaa ggtcaggtgc ggcacctgtc cgcccaccgg cagcgtgaag ctgacgttgt
     3661 cggccgtcgg gatgccggca taagcctcga tgtcgcgctc gatgcggcgc gccgtgttgt
     3721 ggttgacgtc caggcttgcg cgccaggcgt cgttgaactt gaactcgctg cgccaaccca
     3781 tcgactgcag cgtgtcgtcg tcgaagacgt tctcggtgta gtcgatgatg ccgttgggat
     3841 tgccgccgag gcggtaggtg cccgaggtga cctggttcgt cgtcggatcg accgtcgcgt
     3901 catagacgtc gatcggcagc ggcgccttca gcgtcgtgtg gtgcgtgtcg gtgttgatct
     3961 gggcgtggaa gatgtccagc tcggtgcgga accggtcgtt cggcttgaat tccaggatgg
     4021 cggcgaggcc gttgcgcttg tcggagatgt tggcgctgtc gaagcccatg ccgccgctga
     4081 agggcagcgt ggcaccggcg atcgtggcgc tgtcgtcgcc gaccgggtgc gcttgcagcg
     4141 gcttcttcgg atcggtcgcc cagctcgaga cgccgagctg gctgctactg ccatcgaccc
     4201 ggacgaagcc gagcgcgacg ccgatcttgc ggtcggcgaa ctggtcgatg taggtgatgc
     4261 tctcgcgcgt ctcgttgccg tgaggcttca ggccgacgct cggatgaccc ttctggccgc
     4321 tgacggcgac gacgcgattc gggaaggcga gcgggtcgac cagcttgttg tcgatcgtgc
     4381 ccgccaggcc cgccgtcgcg aggctcgcgt cgctggtctt gtacacggtc gcgccggcga
     4441 tcagctcggc cgggtagacg ctcaggtcga cgccgcggct gtcggtggtc gaggtctgct
     4501 cgcggccgtt gagcaggtag ccgttgaagt cggagctcag gccgcggatg ttgacgttcg
     4561 ttgccttgcc ggtgctgtcg cgctgcgacg tcacgcccgg caggtgcgcg agcgactcgg
     4621 cgatcgtcgt gtccggcagc ttgccgatgt cttccgccga gatcgcctcg acgatctggt
     4681 cggagttctt cttgaccgag atcgcggcct cgatgccctt gcggatgccg acgaccgtca
     4741 ccgactggcc gaccgtcggc tcgctggcgg ccggctgcgg cgccgcggcg gctccctgcg
     4801 cgaacaccgt gccggacgcg gccagcgcca gggcgcccgc ggtggcctgg ccggcgagaa
     4861 ccatggtcgg cttcaacttg aacagggccg agcctgcacg ggtagcggaa gagtttttca
     4921 ccagctgtct cctaagttct ttcggggtgg cggtggatcg cgcccagacg ataagcactc
     4981 tttgtagtca aacgaccctc acccaccgaa ggccgagaac cgaatgtaat gaaactagat
     5041 ttatcggttc atgagcggaa gtttaccgaa gttttcctgt tgcgcaaccg tcttttcctg
     5101 gggaagccgc ccatttcatg gccagagaca cgaggggaaa cacgggggtg tccgccatgc
     5161 cgtgccccct ccggcaactt ggcaacggtt ccatcatgta gtccaattac catcaaacgg
     5221 gcgtttttgt agcttcctga ccgaccggcg tggggtccgg gatggacgcc gtcaccgccc
     5281 cgcgcgccaa aaaagcgctg cgttggcccg acaatcgacg catgaatgcg gaaagcaccc
     5341 ccgacgtgaa agacagcccc tggtcgcccg cggcggccgc caacgccgag ccgcggctcc
     5401 tggccgacat cggcgccacc tacgcgcgct tctgcgtgga ggagtcgccc gggcacttcg
     5461 accacgtcgt cgtcctgcca tgcgccgact tcgccggttt cgcagacgtc gtgcaggcct
     5521 accttcgcgg actcgagggc acgccgccgc gccacgcggc gatcgcgatg gccaacccga
     5581 tcgccggcga ccgcgtgcag atgaccaacc gcgactggtc gttctcgatc gagcaggtgc
     5641 gccagcagct cggcttcgcg accttcctcg tcgtcaacaa cttcacggcg ctggcgatgg
     5701 cgctgccggg actggacggc acccagcgcg tccaggtcgg cggcggcgag gcgcagggcg
     5761 acggcgtcat cgccctgctc ggccccggca ccgggctggg cgtgtccggg ctgatcccgg
     5821 ccgacgaccg ctggtcgacg ctcgccaccg aaggcggcca cgtcagcttc gcgcccaacg
     5881 acgagcgcga gctgcgcgtc ctgcagcacg cgtggaagca cctgccgcac gtctcggccg
     5941 agcgcctggt ctccggcccc ggcatcgagc tgatccacga ggcgctcacg cagggcaccg
     6001 cgcaggccgg ccagcgcctg acgacggccg agatcgtgcg ccgcgccgtc acgcgcgccg
     6061 acccggcctg cgccgagacc gtcgactgct tctgcggcat gctgggcacc gtcgcgtccg
     6121 acctcgcgct gacgctcggc gccacgggcg gcgtctacat cggcggcggc atcgtgccgc
     6181 gcctgggcga gctgttcgag cgctcctcat tccgcacgcg cttcgaatcc aagggccgct
     6241 tcagcgcgtg gctggcgaag atcccgacct tcgtgctgac cgccgacaac ccgactttcc
     6301 gcggcctgtc gctgctgctg gccgagcacg tgcccgacac cagctcgccg cacagccccc
     6361 tgctcgaccg cgtgcgcgcc tcgcgcgaca cgctgacgcc ggccgagcgc cgcgtggcgg
     6421 acgtcgtgct gggcaacccg cgctcgctgc tgaacgacgc gatcgccgtc atcgcgcaac
     6481 aggccgaggt cagccagccc accgtcatcc gcttctgccg ctcgctcggc ttcgaaggcc
     6541 tggccgactt caagctgaag ctcgcgtcag gactgaccgg aacgatcccc gtgctgcgca
     6601 gccaggtgcg gcgcggcgag ccgacggccg acctcagcgc caaggtgctg gacaacacga
     6661 tctccgcgat cgtgcacttc cgcgagtcgc tcaacgcggc cgccatcgac cgcgcgatcg
     6721 ccctgctgcg cggcgcgcgc cgcgtcgaga tcttcgcctg cggcaactcc gcggtcgtcg
     6781 cgctcgacgc ccagcacaag ctgctgcgct ttcgcatccc ggtcgtcgcg cagaccgata
     6841 cctcgctgca gagcctggcc gcggagatgc tgtcgcccgg cgacgtcgcg gtcttcatct
     6901 cgagttccgg ccagaccgcg gagctcgtgc gcgccgccgg cgtcgcgatc gagcgcggcg
     6961 ccagcgtggt cgccatcacc gcaagccagt cgcgcctcgc ccgcctcgcg acggtctgcc
     7021 tgggcctcga ccacgccgag gacagctcga ccttcctctc gatggtcgcg cgcatcctgc
     7081 agctgctggt gatcgacatc atgtcggtcg gcctggccgt gcagcgcgac acgtcgtcga
     7141 cgcgcgagcc cggcgacggc gcctcgcgca cgacgcccgc gccgggggtg cggatctcgc
     7201 acctgagcta aggcgcgatc cggccaagaa aaacggcccg gagcaccggg ccgcccttgc
     7261 cagcacgcgc gggcgcgcgt cacgcacccg cgcgcccggt catttcgcgt cgcgcacgca
     7321 gtcgacgtag tagtgcttgc ggccgaagct gtcctcctcg atcaccagcc cgtggacgtc
     7381 ggtgtcgaag cccgggaagc gctcgttgaa ggcacgcgcg aactgcagat aggcgaggat
     7441 cttgcggttg aagcgctcgc ccgggatcag cagcgggatg cccggcgggt acggcgtcag
     7501 cagcgaggtc gtgatgcggc cctcgaggtc gtcgatggcc acgcgctcgg tgtcgcggtg
     7561 ggcgatgcgg gcgtaggcct cggacggctt catcgcgggg tgcaggtccg acaggtacat
     7621 gtcggtggtc aggcgcgcga tgtcgccctc ggtgtagacc tcgtggatcg cctggcacag
     7681 gtcgcgcagg cccatgcgct cgtagcggcg gtgggcgttg cagaactccg gcaggatgcg
     7741 ccacagcggc gcgttcttgt cgtagtcgtc cttgaactgc tgcagcgtgg ccagcagcgt
     7801 gttccagcgg cccttggtga tgccgatcgt gaacatgatg aagaacgagt acaggcccgt
     7861 cttctccacc acgatgccgt gctccgcgag gtacttggtg acgatggacg cggggatgcc
     7921 gtccttcgag aagttgcccg agacgtccag ccccggcgtg atgatggtgc tcttgatcgg
     7981 gtcgagcatg ttgaagcccg gcgccaggtt gccgaagccg tgccacgact cgtcgtcggg
     8041 ctcgagcacc cagtccgccg cctcgccgat gccttcctcg gcgaacttct ccggccccca
     8101 gaccttgaac caccagtcgc cggagccgaa ctcctgctcg atcttgcgca tcgcgcggcg
     8161 gaagtcgagg gcctccttca ggctctcctc gaccagcgcg ttgccgccgg gcggctccat
     8221 catggcggcg gcgacgtcgc acgacgcgat gatcgcgtac tgcggcgagg tcgacgtgtg
     8281 catcaggtag gcctcgttga agaggtggcg gtcgagcttg cgcgtctgcg agtcctgcac
     8341 cagcacctgc gaggcctgcg acaggccggc gagcagcttg tgcgtcgact gcgtcgcata
     8401 gaccatcgcc tccttcggcc gcgcgcgcgc cttgcccatc gcgtggtaga cgctgtagaa
     8461 gtcgtggaag gccgcgtgcg gcaaccaggc ctcgtcgaag tgcagcgtgt cgatccagcc
     8521 gtccagcagc gacttgatcg tctccgtgtt gtagacgacg ccgtcgtagg tgctctgcgt
     8581 gagcgtcatg atgcgcggct tgaccgcctt cgggtcgacg ccctcgagca gcgggttggc
     8641 ggcgatcttg cgctcgatgg cctcgcgcgt gaactcggac tgcgggatcg ggccgatgat
     8701 gccgtagtgg ttgcgcgtcg gcgtcaggaa cacgggcacc gcgccgcaca tgatgatgga
     8761 gtgcaggatc gacttgtggc agttgcggtc gaccaccacg acgtcgcccg gcgccaccgt
     8821 gtggtgccag accatcttgt tggacgtgct cgtgccgttg gtgacgaaga agcagtggtc
     8881 ggcgttgaag atgcgcgcgg cattgcgctc gctgtccgcg accggccccg tgtggtcgag
     8941 gagctggccg agttcctcga ccgagttgca gacgtcggcg cgcagcatgt tctcgccgaa
     9001 gaactggtgg aacatctggc cgaccgggct cttcaggaac gccacgccgc ccgagtgccc
     9061 cggacagtgc cacgagtacg agccgtcggc cgcgtagtcc atcagcgcct tgaagaacgg
     9121 cggcgccacg ccgtcgaggt agctgttggc ctcgcggatg atgtggcgcg cgacgaactc
     9181 cggcgtgtcc tcgaacatgt ggatgaagcc gtggagctcg cgcaggatgt cgttggggat
     9241 gtgctgcgac gtgcgcgtct cgccgtacag gtagatcggg atctcggggt tcttcgagcg
     9301 gatctcctgg atgaacttgc gcaggttgag caccgccggg tcgagctcgg gcccgggggt
     9361 gaattcctcg tcgtcgatgg acaggatgaa cgccgacgcg cggctttgct gctgcgcgaa
     9421 ctgcgacagg tcgccgtagc tggtcacgcc caggatctcg atgccctcct tctcgatcgc
     9481 ggacgcgagc gcccggatgc cgagcccgga ggtgttctcc gagcggaagt cttcatcgat
     9541 gatgacgatg ggaaagcgga aacggagcat ggcacgaggc ccgcgcgtcg tgcgggcatg
     9601 gcggacggga aacgacgtgg gaaccggccg tgagccgggc ggcgccggga cgcgaaagcg
     9661 gggtggaaaa ccggcgaagg gcgaagtgta gggccgaact cttgcagcgc aatgtccgcg
     9721 ccgcgacgcc gccccgcacc gcgccggata tgcaacacat ggcctccgcc ccgccccgtc
     9781 gccacccgcc gcgcagccgc tttgcaggcc gcgcgaaacc tgagctagaa tcgagggcta
     9841 ccccggaagg gtggatgagc ggtttaagtc gcatgcctgg aaagcatgtg taggttaata
     9901 gcctaccgcg ggttcgaatc ccgccccttc cgccaggaaa ctccctctct aacgcgcaac
     9961 agaatgccgt tagcacgcag cgagagattc ctaagtcgtt gatttgacga cactttctcc
    10021 cctcgcacca ctgtctgacg cgcaacgcga tgtcgtttgc gcgcggtggc ccgtcgcctg
    10081 ttcctggcgg actggctggc agacagcgtg gcggaatggg ggacggcgga tggcccgcaa
    10141 gcgcatcgtt tcgaacctgc accggctcgc ggtgcgggag gttcaggcgg ccggcgacgg
    10201 cgaccacgcg gatggcggcg gactgctgct gcgcgcgcgc ggccagtccg ccagctgggt
    10261 cttccggtac acggccgcga ccgggcgccg gcgcgagatg gg
//
LOCUS       DASVLN010000627         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_87101, whole genome shotgun sequence.
ACCESSION   DASVLN010000627 DASVLN010000000
VERSION     DASVLN010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87101"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..750)
                     /locus_tag="VF453_15015"
     CDS             complement(10..750)
                     /locus_tag="VF453_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689023.1"
                     /translation="MAIAQAGLAFRARAPIEDLNAVLAQLFDDDVAEVASPVSARFDI
                     RNRSDVMVQLFGDVCFVSNDELAWPLLAQEDHDARRVHASLGAPALMLAFCRYDAGGS
                     HGYAVFEHGRRVRRRLQTLDVPGLAPLAESGAPLPFEQRWLKAAHYIEAEARPADGRA
                     PVRDEGKVYYLGDREVLVPARELTGRMLHDGLEALFGVCPWDTLITPTYRFFRVGRRP
                     EREPVTHDGEPPSASAGRRARRSWWRLR"
     gene            complement(844..>1846)
                     /locus_tag="VF453_15020"
     CDS             complement(844..>1846)
                     /locus_tag="VF453_15020"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7689024.1"
                     /translation="VRDACARLGRGPWRRDGLFGAFYAAASWALSLPLTIWHDYFREH
                     AYGMANQTFGPWFAQQLLGAAVGVVVCAIAVPVFYAAVRRAGERWWLWGTGVVMVLAT
                     FGIAAGPAWIDPLFNTYKPVADPVVREAVLHLARIDGVPVHDVLQFDASRQTTQVSAN
                     VAGLFGTASVRLNDNLLRCSLPEIRAVTGHELGHYVLNHIEKSVCELAILTFCGFLFA
                     QWAMGRLLATAGARWRLHGVQDVASLPLLAAVMSVFMLLSMPVANTLTRTQEIEADRF
                     GLNLAREPHGAAEAYLKLTEYRKPDPGPIEEFIFFDHPGTRFRIHDAMRWRQEMGTP"
BASE COUNT          267 a          655 c          691 g          233 t
ORIGIN      
        1 cgggcgcggt cagcgcagcc gccaccacga ccgccgcgcg cgccggccgg ccgaggccga
       61 gggcggctcg ccatcgtggg tgacgggttc gcgctcgggc cggcggccga cgcggaagaa
      121 gcggtaggtc ggcgtgatca gcgtgtccca cgggcagacg ccgaacagcg cctccaggcc
      181 gtcgtgcagc atgcggccgg tgagctcgcg cgcgggcacg agcacctcgc ggtcgcccag
      241 gtagtacacc ttgccctcgt cgcggacggg cgcgcggccg tcggcgggcc gggcctccgc
      301 ctcgatgtag tgcgccgcct tcagccagcg ctgctcgaac ggcagcggcg cgccgctctc
      361 ggccagcggg gcgaggccgg ggacgtcgag cgtctgcagg cgcctgcgga cgcggcggcc
      421 gtgctcgaac accgcgtagc catggctgcc gccggcgtcg tagcggcaga aggccagcat
      481 cagcgccggc gcgccgagcg aggcgtggac gcggcgggcg tcgtggtcct cctgggcgag
      541 cagcggccag gcgagttcgt cgttgctcac gaagcagacg tcgccgaaca gctgcaccat
      601 gacgtcggag cggttgcgga tgtcgaagcg cgcggacacg ggcgacgcga cctcggcgac
      661 gtcgtcgtcg aagagctgcg ccaggacggc gttgagatcc tcgatcggcg cccgcgcgcg
      721 gaacgcgagg ccggcctgtg cgatggccat gtgctgcggg acgggcgggg cgccctcgtg
      781 gcgatgggcg caaggttagc gcatcgcgct gccgcttgtg cgcgggcttg cccgcgcgtc
      841 cgttcagggc gtgcccatct cctggcgcca gcgcatcgcg tcgtggatcc ggaaacgcgt
      901 gccgggatga tcgaagaaga tgaactcctc gatcgggccc gggtcgggct tgcggtattc
      961 cgtcagcttc aggtaggcct cggccgcgcc gtggggctct cgcgccaggt tcaggccgaa
     1021 gcggtcggcc tcgatctcct gggtgcgcgt cagcgtgttt gccacgggca tgctgaggag
     1081 catgaagacc gacatcaccg ccgcgagcag cggcaggctg gcaacgtcct gcaccccgtg
     1141 cagccgccag cgcgcgccgg cggtcgccag cagccggccc atcgcccact gcgcgaacag
     1201 gaagccgcag aaggtcagga tcgccagctc gcacaccgac ttctcgatgt ggttcaggac
     1261 gtagtggccc agctcgtggc cggtgacggc gcggatttcc ggcagcgagc agcgcagcag
     1321 gttgtcgttc agccgcacgg acgccgtgcc gaacaggccc gcgacgttgg cgctcacctg
     1381 cgtggtctgg cgcgatgcgt cgaattgcag gacgtcgtgc acgggcacgc cgtcgatgcg
     1441 cgccaggtgc agcacggcct cgcgcaccac ggggtcggcg accggcttgt aggtgttgaa
     1501 cagcggatcg atccaggccg gccctgccgc gatcccgaac gtcgccagca ccatcaccac
     1561 gccggtgccc cacagccacc agcgctcgcc ggcccgccgc accgcggcgt agaacaccgg
     1621 caccgcgatc gcgcagacga cgacgcccac cgcggcgccg agcagctgct gggcgaacca
     1681 cggcccgaag gtctggttgg ccatgccgta ggcgtgctcg cggaagtagt cgtgccagat
     1741 cgtcagcggc aacgacaggg cccagctcgc cgcggcgtag aacgcgccga acagcccgtc
     1801 gcgccgccac ggcccgcggc cgaggcgcgc gcacgcgtca cgcacg
//
LOCUS       DASVLN010000628         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_247648, whole genome shotgun sequence.
ACCESSION   DASVLN010000628 DASVLN010000000
VERSION     DASVLN010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247648"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1193)
                     /locus_tag="VF453_15025"
     CDS             complement(135..1193)
                     /locus_tag="VF453_15025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7689025.1"
                     /translation="MTANTAAARAFGWVEQGLVPDGVVRLGIRRLLRERLDEVGADDT
                     EAAAAIAQVFVDGLREAPLALVPEKANEQHYEVPAAFFAKVLGPHRKYSSCLWPPGVD
                     DLAGAEAAALAQTCERAGLADGQEILELGCGWGSLSLWMAERWPASRITALSNSHSQR
                     EHIEAQAAARGLGNLRVVTRDINAFEGDAGRYDRVVSVEMFEHLRNWPLAFARVARWL
                     APGGRFFMHVFAHRGAPYAFVERDASDWMSRHFFSGGMMPSDDLAATFQDDLRLLRRW
                     RWDGRHYQKTAEAWLRNLDAARDELAPLMRATYGDAADVWWQRWRLFFLSVSELFGHA
                     DGQQWFVGHYLFEPRARA"
     gene            complement(1270..>1525)
                     /locus_tag="VF453_15030"
     CDS             complement(1270..>1525)
                     /locus_tag="VF453_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7689026.1"
                     /translation="AARVRRLAELRAAEGYMARAEKHGRDWLLIEDHCPICAAATACQ
                     GFCRSELALMQRSLGDRVRVERVEHVLAGARRCAYRVSAA"
BASE COUNT          209 a          567 c          548 g          201 t
ORIGIN      
        1 cacgccattc gcatcctggc gcgcgcctgg ctgcgcgtcc tctggcgtgc ctggaccacg
       61 catcgtccct acgatgcctc gctccacggc gctgccgctc ggcttgccta gcaagggggt
      121 tgacacaggc tgtctcatgc cctcgccctc ggctcgaaca ggtagtggcc gacgaaccac
      181 tgctggccgt ccgcatggcc gaacagctcg gacaccgaca ggaagaacag ccgccagcgc
      241 tgccaccaca cgtcggccgc gtcgccgtag gtcgcccgca tcaggggcgc gagctcgtcg
      301 cgcgcggcat cgaggttgcg cagccacgcc tcggcggtct tctggtagtg ccggccgtcc
      361 cagcgccagc gccgcagcag ccgcaggtcg tcctggaacg tggcggcgag gtcgtcggag
      421 ggcatcatgc cgccggagaa gaagtggcgg ctcatccagt cgctggcgtc gcgctcgacg
      481 aacgcgtacg gcgcgccgcg gtgcgcgaac acgtgcatga agaaccgccc gcccggcgcg
      541 agccagcgcg ccacgcgcgc gaaggcgagc ggccagttgc gcaggtgctc gaacatctcg
      601 accgagacga cgcggtcgta gcgcccggcg tcgccttcga acgcgttgat gtcgcgcgtg
      661 accacgcgca ggttgcccag accgcgcgcg gcggcctgcg cctcgatgtg ctcgcgctgg
      721 gagtgcgagt tggacagcgc cgtgatccgg ctcgcgggcc agcgctcggc catccacagg
      781 ctgagcgagc cccagccgca gccgagctcg aggatctcct gcccgtccgc caggccggcg
      841 cgctcgcagg tctgcgcgag cgccgcggcc tccgcgccgg cgaggtcgtc gacgcccggc
      901 ggccacaggc agctgctgta cttgcggtgc gggcccagca ccttggcaaa gaacgcggca
      961 ggcacctcgt aatgctgctc gttggccttc tccgggacga gggcgagcgg cgcctcgcgc
     1021 agcccgtcga cgaagacctg cgcgatcgcc gcggcggcct ccgtgtcgtc ggcccccacc
     1081 tcgtccaacc gctcgcgcag caggcggcgg atgcccaggc ggacgacgcc gtcgggcacg
     1141 aggccctgct cgacccagcc gaaggcgcgg gcggcggcgg tgttggcggt catgcggcgg
     1201 gctcctccat cgatgcggat gtcggggata cggacgaccg cgccgatcgg attcgttcgc
     1261 gcgttctcct caggcagcgc tcacgcgata ggcgcaacgc ctcgcgccgg cgaggacatg
     1321 ctcgacgcgc tcgacgcgca cgcggtcgcc caggctgcgc tgcatcagcg cgagctccga
     1381 gcggcagaag ccctggcagg cggtcgccgc ggcgcagatc gggcagtggt cctcgatcag
     1441 cagccagtcg cggccgtgct tctcggcgcg cgccatgtag ccctcggcgg cgcgcagctc
     1501 ggcgagccgg cgcacgcgcg cggcc
//
LOCUS       DASVLN010000629         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_247658, whole genome shotgun sequence.
ACCESSION   DASVLN010000629 DASVLN010000000
VERSION     DASVLN010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247658"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..943)
                     /gene="rpe"
                     /locus_tag="VF453_15035"
     CDS             complement(260..943)
                     /gene="rpe"
                     /locus_tag="VF453_15035"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX7689027.1"
                     /translation="MKPTYRIAPSILSADFARLGQEFADVIAAGADWIHFDVMDNHYV
                     PNLTFGPMVCQALKKHAVKADGTPVPVDVHLMVQPVDALAQAFAKSGADIVTFHPDAS
                     THVDRTLQLIKAEGCKAGLVFNPAAPVDVLEWVVDKVDVVLVMSVNPGFGGQSFIPSA
                     LRKIERLRRIIDASGRDIRLEVDGGIKADNIRAAADAGADTFVAGSAIFGQRDYARAI
                     AEMRAALAA"
     gene            1033..1407
                     /gene="apaG"
                     /locus_tag="VF453_15040"
     CDS             1033..1407
                     /gene="apaG"
                     /locus_tag="VF453_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX7689028.1"
                     /translation="MSRPELTCEVATRHLPDQSSAQDDVYAFAYTITIRNTGDVAAQV
                     IGRHWVITHGSGETEEVRGLGVVGQQPLLQPGEQYEYTSWTRLTTPRGSMRGEYFCMT
                     EDARWFEATIPEFALAIAQALH"
     gene            complement(1441..>1658)
                     /locus_tag="VF453_15045"
     CDS             complement(1441..>1658)
                     /locus_tag="VF453_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689029.1"
                     /translation="GPIGTEEQPASVPRIADRTTTARRTLMGWIVFEALLALAAGLLV
                     VWWTFRSRREPDEPAADEPAGAPDERL"
BASE COUNT          235 a          589 c          589 g          245 t
ORIGIN      
        1 cacgccattc gcatcctggc gcgcgcctgg ctgcgcgtcc tctggcgtgc ctggaccacg
       61 catcgtccct acgatgcctc gctccacggc gctgccgctc ggcttgccta gcaagggggt
      121 tgacacaggc tgtctcatgc catgagcctg ggtcgtgcga gcggccggga tccttacgcg
      181 gcgccgaaaa aggaaagccg cgacgcccgc cggtcggcag gtcgtcgcgg ctcgttccgc
      241 gggcgcgggc gggtcgacgt caggccgcga gggccgcgcg catctccgcg atcgcccggg
      301 cgtagtccct ctggccgaag atcgcgctgc ccgcgacgaa ggtgtcggcc cccgcatcgg
      361 ccgcggcgcg gatgttgtcg gccttgatgc cgccgtcgac ttcgaggcgg atgtcgcggc
      421 ccgacgcgtc gatgatgcgg cgcaggcgct cgatcttgcg cagcgcgctc gggatgaagc
      481 tctggccgcc gaagccgggg ttgacgctca tcacgagcac gacgtcgacc ttgtcgacca
      541 cccattcgag cacgtccacc ggcgccgccg ggttgaagac gagccccgcc ttgcagccct
      601 cggccttgat gagctgcagc gtgcggtcga cgtgcgtgct cgcatcgggg tggaaggtga
      661 cgatgtcggc gcccgacttc gcgaacgcct gggcgagcgc gtcgaccggc tgcaccatca
      721 ggtggacgtc gaccggcacc ggcgtgccgt ccgccttgac cgcatgcttc ttgagcgcct
      781 ggcagaccat cggcccgaac gtcaggttcg gcacgtagtg gttgtccatc acgtcgaagt
      841 ggatccagtc ggcgccggcc gcgatcacgt cggcgaactc ctggccgagc cgcgcgaagt
      901 cggcggagag gatggacggg gcgatgcgat aggtgggctt catggctcgg ggaatcgtgc
      961 ggtggcgggc gatccgcgat tttaggaggc cgccggcagc cgcccgcccg cggcatgccg
     1021 cagaatgcac ggatgtcccg ccccgaactc acctgcgagg tcgccacgcg acacctgccg
     1081 gaccagtcct ccgcgcagga cgacgtctac gcgttcgcct acacgatcac gatccgcaac
     1141 accggcgacg tcgccgccca ggtcatcggc cgccactggg tcatcaccca tggcagcggc
     1201 gagaccgagg aggtgcgcgg cctgggcgtc gtcggccagc agccgctgct gcagccgggc
     1261 gagcagtacg agtacacgag ctggacgcgc ctgacgaccc cgcgcggcag catgcgcggc
     1321 gagtacttct gcatgaccga ggacgcgcgc tggttcgagg cgacgatccc cgagttcgcg
     1381 ctcgcgatcg cgcaggcgct gcactgacgg ccgcgcggaa ctccgggcgc cgtgcggcgc
     1441 tcagaggcgt tcgtccggcg ccccggccgg ctcgtcggcg gcgggctcgt cgggttcgcg
     1501 ccgcgagcgg aacgtccacc agaccacgag cagccccgcg gcgagcgcca gcaacgcctc
     1561 gaacacaatc cagcccatga gagtccgcct cgccgtcgtt gtcctgtccg cgattctagg
     1621 gacgctcgcg ggctgttcct cggtgccgat cgggccgg
//
LOCUS       DASVLN010000630         5681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_142629, whole genome shotgun sequence.
ACCESSION   DASVLN010000630 DASVLN010000000
VERSION     DASVLN010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142629"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VF453_15050"
     CDS             complement(<1..454)
                     /locus_tag="VF453_15050"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX7689030.1"
                     /translation="MPARRRPSSTRIAGVEFEAPAEEVARALIGVTLLVDGVGGVIVE
                     TEAYDQSEPAAHTHRGPTPRNAPMFGPAAHAYVYRSYGLHWCLNLTCREVGHGAGVLI
                     RALAPTQGLDRMRERRGVEDARLLCSGPGRVAQALGITLAHFGLPADRA"
     gene            complement(454..840)
                     /locus_tag="VF453_15055"
     CDS             complement(454..840)
                     /locus_tag="VF453_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7689031.1"
                     /translation="MPRQDTLDRFVAMVESNAHDRACEEFYAEHSTMQENQAAPRLGR
                     DRHVANERAVMARAKTMSSRCVRPVLVNGDHVVIRWIFRFEWLDGSVTQMEELAWQRW
                     QDERIVEETFFYDPAQRVPKRPEQAV"
     gene            complement(979..1644)
                     /locus_tag="VF453_15060"
     CDS             complement(979..1644)
                     /locus_tag="VF453_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HEX7689032.1"
                     /translation="MISLFPRGRRGSVAVAAGLAWALAAAAPAQTVTYNGMLGDRALL
                     VIDGQAQAVAVGATARGLKLVRVDGGHAQVEIGGKLLSLQLGGSFQAPGSEAGGNGSR
                     IVLAAGPGGHFVGTATINGHPARFMVDTGATAVSIGRDEADRLGLDYAHGGFGGAMTA
                     NGPIAVRTITLTTVRVGDVLVADVPATVVPQPMPYVLLGNSFLARFRMERDNDTMVLE
                     KKH"
     gene            1911..3482
                     /locus_tag="VF453_15065"
     CDS             1911..3482
                     /locus_tag="VF453_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX7689033.1"
                     /translation="MERPRLDHKPGAATWLVFIALLVAGLGYTAWSIATDYVGSGERS
                     TAVWPFILLGLALLIALGFEFVNGFHDTANAVATVIYTNSLPPQFAVVWSGIFNFLGV
                     LVSSGAVAFGIISLLPVELILQVGSSAGFAMVFALLIAAILWNLGTWWLGLPASSSHT
                     LIGSIIGVGVANALMHGRDGTSGVDWSQAVKIGYSLVLSPLVGFVFAALLLWALRVFV
                     KNKALYDAPRGSAPPPWWIRGLLVLTCTGVSFSHGSNDGQKGMGLIMLILVGTVPISY
                     ALNRAMPADETTQFAIVAHATQQKLATVAPGVVAADPRAALSTYVRTKVVAPETVPAL
                     AAISGSIGDQVQRHATLARMPAASVANVRNDMYLASEAIRLLSKDAHVAFDVDTKATL
                     GTFKNRIDSATKFIPLWVKVAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYAQGMSA
                     EVVAMLTIGAADNFGLPVSTTHVLSSGIAGTMAANRSGLQWGTLRNLALAWVLTLPAA
                     IALSGALYWLFAHLF"
     gene            3496..4404
                     /gene="ppk2"
                     /locus_tag="VF453_15070"
     CDS             3496..4404
                     /gene="ppk2"
                     /locus_tag="VF453_15070"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX7689034.1"
                     /translation="MNALFDQETMRRRIERELVDGYDEEIEMEMEDRLVADPLDASGE
                     DAAGRVKYFRELFRLQGELVKLQDWVVATRQKAVILFEGRDAAGKGGVIKRITQRLNP
                     RVCRTVALPAPNDRERTQWYFQRYAAHLPAAGEIVLFDRSWYNRAGVERVMGFCTDEE
                     YEDFFRSVPEFERMLQRSGIKLIKYWFSITDDEQHLRFLSRIHDPLKQWKLSPMDLES
                     RRRWEEYTKAKEVMLERTHSAESPWWVVQAVDKKAARLNCIHHLLTQFPYAEIEHTEV
                     VLPERIRHEHYSRQAVPAEMMVPEVY"
     gene            complement(4427..5485)
                     /gene="pstS"
                     /locus_tag="VF453_15075"
     CDS             complement(4427..5485)
                     /gene="pstS"
                     /locus_tag="VF453_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX7689035.1"
                     /translation="MTTTPLRVGALLLACLAVLASPARAAAEVHGAGATFPSAVYKAW
                     ASGYEQARGTKVSYQPTGSGDGIKRIEAREVDFGASDSALSQADLESHGLLQIPSAIG
                     GIVPVVNVRGVANERLRLTGELLADIMRGAVDKWNDRRIAALNPDVELPPVAIVRVVR
                     AEKSGTNDAFTRYLSLSSPEWKRQVGSGQLVKWPGTVTAVDGNDGVARTVAATAGAIG
                     YVSYDRVAQYRLAGVRMRNRAGGFVSPSPEGFRAAVKESDLNRKDDETATLLDQPGPQ
                     TWPLTITTYLLLDVHPKTAEGARGALQFVWWSLLNGDGVVRSTGLTPMPETIQARLVQ
                     RFARIKPQDGQPLNFFSD"
BASE COUNT          791 a         2048 c         2023 g          819 t
ORIGIN      
        1 gcgcgcggtc ggccggcaac ccgaagtgcg ccagcgtgat gcccagcgcc tgcgcgacgc
       61 ggcccgggcc cgagcacagc agccgcgcgt cctcgacgcc gcgccgctcg cgcatgcgat
      121 ccagcccctg ggtcggcgcg agcgcgcgga tcagcactcc cgccccgtgc cccacctcgc
      181 ggcacgtcag gttgaggcac cagtgcaggc cgtaggagcg atacacgtac gcatgcgccg
      241 ccggcccgaa catcggcgcg ttgcgcggcg tcggcccgcg gtgcgtgtgc gcggccggct
      301 cggactggtc gtaggcctcg gtctcgacga tcacgccgcc cacgccgtcg accagcagcg
      361 tcaccccgat cagcgcccgc gcgacctcct cggcgggggc ctcgaactcg acgccggcga
      421 tgcgggtgga ggacgggcgc cggcgtgccg gcatcagacg gcctgctccg gccgcttcgg
      481 cacgcgctgc gccggatcgt agaagaaggt ctcctcgacg atgcgctcgt cctgccagcg
      541 ttgccaggcg agttcctcca tctgcgtgac gctgccgtcc agccactcga agcggaagat
      601 ccagcggatc acgacatggt cgccgttcac gaggacgggc cgcacgcagc gcgaggacat
      661 cgtcttcgcg cgcgccatca cggcgcgctc gttggcgaca tgcctgtcgc gaccgaggcg
      721 cggcgcggcc tggttctctt gcatcgtcga gtgctcggcg tagaactcct cgcaggcgcg
      781 gtcgtgggca ttgctctcga ccatcgcgac gaagcggtcg agggtgtcct ggcggggcat
      841 gcgcgatcct tgtgaagcgg agatcgggcg attctggcgg ccggcgcgtg gccgcgtcgg
      901 ccgcaagggc atggccggca tcgaggagac gcctggtcga cgcgcgcggc cccgctcgcg
      961 ccgcggggcg gcgcccggtc agtgcttctt ctcgagcacc atcgtgtcgt tgtcgcgctc
     1021 catgcggaaa cgcgcgagga agctgttgcc aagcagcacg tagggcatgg gctgggggac
     1081 gaccgtggcc gggacgtcgg cgacgagcac gtcgcccacg cgcaccgtgg tcagcgtgat
     1141 cgtgcgcacc gcgatcggac cgttggccgt catcgcgccg ccgaagccgc catgggcgta
     1201 gtccaggccg agccggtcgg cctcgtcgcg gccgatcgag acggcggtcg cgccggtgtc
     1261 caccatgaag cgggcggggt gcccgttgat cgtcgcggtg ccgacgaagt gaccgcccgg
     1321 ccccgccgcg aggacgatgc gcgagccgtt gccgcccgcc tcgctccccg gcgcctggaa
     1381 cgagccgccc agttgcaggc tcagcagctt gccgccgatc tcgacctgcg catggccgcc
     1441 gtccacgcgg accagcttca ggccgcgcgc cgtcgcgccg acggccaccg cctgcgcctg
     1501 gccgtcgatg accagcagcg cgcgatcgcc gagcatgccg ttgtaggtga ccgtctgcgc
     1561 gggtgccgcg gcggccagcg cccaggccag cccggcggcc accgcgacgc tgccgcgccg
     1621 gccgcgcgga aatagcgaga tcatgcgttc ctcccctgcc gtcgccagcg gcctcccgtc
     1681 cggtggccag tctaacgcgg cgccggcgtc gcgcgtcgat ggccgggcgc cgggagattc
     1741 ccggtcgacg gcgatgccgg ggccgcgggc accccggccc cgacccgcgc ctgtcacccc
     1801 gctgtcacgc cccgacgcga ccatccgcgg acgtccgacc ggacgaccac aaggacgagg
     1861 gactcgccgt gaacaccaac gtcagcgccg gactgcccgg cgccaccccg atcgagcggc
     1921 cacggctcga ccacaagccc ggggctgcca cctggctcgt gttcatcgcg ctgctggtcg
     1981 cgggcctggg ctacaccgcc tggagcatcg ccaccgacta cgtcggcagc ggcgagcggt
     2041 cgaccgccgt ctggccgttc atcctgctcg ggctcgcgct gctgatcgcg ctcggcttcg
     2101 agttcgtcaa cggcttccac gacaccgcca acgcggtggc cacggtgatc tacaccaact
     2161 cgctgccgcc gcagttcgcg gtcgtgtggt cgggcatctt caacttcctc ggcgtgctgg
     2221 tctcgagcgg cgcggtcgcg ttcgggatca tctcgctgct gccggtggag ctgatcctgc
     2281 aggtcggctc gagcgccggc ttcgcgatgg tcttcgccct gctgatcgcg gccatcctct
     2341 ggaacctggg cacgtggtgg ctcggcctgc cggcgtcgtc gtcgcacacg ctgatcggct
     2401 ccatcatcgg cgtgggcgtg gccaacgcgc tgatgcacgg ccgcgacggc acgagcggcg
     2461 tggactggag ccaggcggtg aagatcggct actcgctcgt gctgtcgccg ctggtgggct
     2521 tcgtcttcgc ggcgctgctg ctgtgggcgc tgcgcgtgtt cgtgaagaac aaggcgctgt
     2581 acgacgcgcc gcgcggcagc gcgccgccgc cgtggtggat ccgcggcctg ctggtgctga
     2641 cctgcacggg cgtctcgttc tcgcacggct ccaacgacgg ccagaagggc atggggctga
     2701 tcatgctgat cctggtcggc acggtgccga tctcgtacgc gctcaaccgc gcgatgccgg
     2761 ccgacgagac gacgcagttc gcgatcgtcg cccacgccac gcagcagaag ctcgcgaccg
     2821 tcgcgccggg cgtcgtcgcc gccgatccgc gcgccgcgct gtcgacctac gtgcgcacga
     2881 aggtggtcgc gcccgagacc gtgcccgcgc tggccgcgat ctccggcagc atcggcgacc
     2941 aggtgcagcg gcacgccacg ctcgcgcgga tgccggccgc gagcgtcgcc aacgtgcgca
     3001 acgacatgta cctcgcctcg gaggcgatcc gcctgctgtc caaggacgcg cacgtcgcct
     3061 tcgacgtcga caccaaggcg acgctgggca cgttcaagaa ccggatcgac agcgccacca
     3121 agttcatccc gctgtgggtg aaggtggccg tcgcgatcgc gctcggcctg ggcacgatgg
     3181 tgggctggaa gcgcatcgtc gtcacggtcg gcgagaagat cggcaagacc cacctgacct
     3241 acgcccaggg catgtcggcc gaggtcgtcg cgatgctgac gatcggcgcc gccgacaact
     3301 tcggcctgcc ggtctcgacg acgcacgtgc tgtcctcggg catcgccggc acgatggcgg
     3361 ccaacaggtc cggcctgcag tggggcacgc tgcgcaacct cgcgctcgcc tgggtgctca
     3421 cgctgcctgc cgccatcgcg ctgtcgggcg cgctgtactg gctgttcgcg cacctgttct
     3481 gacccggaga cccccatgaa cgccctgttc gaccaggaaa ccatgcgccg gcgcatcgag
     3541 cgcgagctcg tcgacggcta cgacgaggag atcgagatgg agatggagga ccgcctggtc
     3601 gccgatccgc tcgacgcgag cggcgaggac gccgccgggc gcgtgaagta cttccgcgag
     3661 ctgttccgcc tgcagggcga gctcgtgaag ctgcaggact gggtcgtggc cacgcggcag
     3721 aaggccgtca tcctgttcga gggccgcgac gcggccggca agggcggcgt catcaagcgc
     3781 atcacccagc gcctgaaccc gcgcgtgtgc cgcaccgtcg ccctgcccgc gcccaacgac
     3841 cgcgagcgca cgcagtggta cttccagcgc tacgccgcgc acctgcccgc ggcgggcgag
     3901 atcgtgctgt tcgaccgcag ctggtacaac cgcgccggcg tcgagcgcgt gatgggcttc
     3961 tgcaccgacg aggagtacga ggacttcttc cgctccgtgc ccgagttcga gcgcatgctc
     4021 cagcgctcgg gcatcaagct gatcaagtac tggttctcga tcaccgacga cgagcagcac
     4081 ctgcgattcc tgagccgcat ccacgacccc ctcaagcagt ggaagctgag cccgatggat
     4141 ctcgagtcgc gccgccgctg ggaggagtac acgaaggcca aggaagtgat gctcgagcgc
     4201 acgcacagcg ccgagtcgcc ctggtgggtc gtgcaggccg tggacaagaa ggccgcccgg
     4261 ctgaactgca tccaccacct cctcacgcag ttcccctacg ccgagatcga gcacaccgag
     4321 gtcgtgctgc cggagcgcat ccgccacgag cactactcgc gccaggccgt ccccgccgag
     4381 atgatggtgc cggaggtcta ctgaccccct gccccgtcca tgcccctcag tcggagaaga
     4441 agttcagcgg ctggccgtcc tggggcttga tgcgggcgaa gcgctgcacc aggcgcgcct
     4501 ggatcgtctc gggcatcggc gtgaggccgg tcgagcgcac gacgccgtcg ccgttgagca
     4561 gcgaccacca cacgaactgc agcgccccgc gcgcgccctc ggcggtcttg gggtgcacgt
     4621 ccagcagcag gtaggtcgtg atcgtgagcg gccaggtctg cggcccgggc tggtcgagca
     4681 gcgtggcggt ctcgtcgtcc ttgcggttca ggtcggactc cttgaccgcg gcgcgaaagc
     4741 cttccggcga cggcgagacg aagccgcccg cgcggttgcg catgcgcacg cccgcgaggc
     4801 ggtactgcgc gacgcggtcg tacgagacgt agccgatcgc gcccgccgtc gcggcgaccg
     4861 tcctggcgac gccgtcgttg ccgtcgaccg cggtgaccgt gcccggccac ttcacgagct
     4921 ggcccgagcc gacctgccgc ttccactccg gcgaggacag cgacaggtag cgcgtgaacg
     4981 cgtcgttggt gccggacttc tccgcgcgca cgacgcgcac gatggccacc ggcggcagct
     5041 cgacgtcggg gttgagcgcg gcgatgcggc ggtcgttcca cttgtcgacc gcgccgcgca
     5101 tgatgtcggc gaggagctcg cccgtcaggc gcaggcgctc gttggcgacg ccgcgcacgt
     5161 tgaccaccgg cacgatgccg ccgatcgcac tcgggatctg cagcaggccg tgcgactcga
     5221 ggtcggcctg gctcagcgcg gaatcggagg cgccgaagtc gacctcgcgc gcctcgatgc
     5281 gcttgatgcc gtcgcccgag cccgtgggct ggtagctgac cttggtgccg cgcgcctgct
     5341 cgtagccgct ggcccaggcc ttgtagaccg cggacgggaa ggtggcgccc gcgccgtgca
     5401 cttcggcggc ggcgcgggcg ggggacgcga ggacggcgag gcaggcgagc agcagcgcgc
     5461 ccacccggag aggagtggtc gtcatggaaa gggggcgttg gggtttccgg cggcgtcgat
     5521 gcggacgctg caccggtgcg ggacgcgtgg agggtcgcgg ccctgctggt tccaacgaat
     5581 tatagggttt acaccatgac acaactgcgg catcgggcca cgagtcgcgg atccggccgc
     5641 gtccgccgcg tccgccgcgt ccgccggggc cgccggggcc g
//
LOCUS       DASVLN010000631         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_32796, whole genome shotgun sequence.
ACCESSION   DASVLN010000631 DASVLN010000000
VERSION     DASVLN010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32796"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF453_15080"
     CDS             complement(<1..552)
                     /locus_tag="VF453_15080"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEX7689036.1"
                     /translation="MSDDELRYFDAHGLDCLTATLHRHRYAPHAHAEYVIGAVVRGEG
                     EIAIAGRRRRVGPGDLTLYNPGEVHDGCARAPGFRYVACYPRPEVVEALVEDAARGGT
                     VRFGPPIVRDPAAARGYLALHRGWESARDPLLVEQGLACFLASCLERHAGVAPAPADH
                     EPAGLARAVDLMHACAGDKLTLAQ"
     gene            complement(832..1200)
                     /locus_tag="VF453_15085"
     CDS             complement(832..1200)
                     /locus_tag="VF453_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7689037.1"
                     /translation="MTDPRTLADRYLATWNEPDAARRSQLLAALWTPDGRYADPMMSG
                     TGPAHIGTLIEAARAQFAGLAFSLRGAPDGHGEFARFSWSLGPAGGEPIAGGTDIARL
                     DAQGRLAEVVGFIDFAPAAA"
     gene            1325..2134
                     /locus_tag="VF453_15090"
     CDS             1325..2134
                     /locus_tag="VF453_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7689038.1"
                     /translation="MNLHASSTAGVGELVREWRQRRRMSQLELAGVADISARHLSFVE
                     TGRASPSRSMLIRLAERLDVPLRARNQMLHAAGYAALYAERPLDAPEMTAARQTVEAV
                     LTAHMPFPALAVDRHWQLVAANEAVGALLAGIDAELLAPPLNVLRLSLHPRGLAPRIA
                     NLGQWRAHLLERLQQQVDRSGDRRLVALHAELVELSPPGPHDLHDHAAPEPGAVAVPL
                     RLRVDDDEPLAFLSTTTVFGTPVDVTLSELALECFYPADDRTRRHLQGARG"
     gene            2176..>2630
                     /locus_tag="VF453_15095"
     CDS             2176..>2630
                     /locus_tag="VF453_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7689039.1"
                     /translation="MPVVIGTAGWNIPHDAADAFDAPDTGSHLERYARVLAGVEVNSS
                     FHRPHRRATWARWAASTPPGFRFSAKLPRAMTHDARLRVDDAALDEFLAQAGGLGDKL
                     AVLLVQLPPSLAFECDVALDFFARLRARTGAAIACEPRHRSWFEAGADAA"
BASE COUNT          329 a         1000 c          978 g          323 t
ORIGIN      
        1 ctgcgcgagc gtcagcttgt cgccggcgca ggcgtgcatc aggtcgacgg cgcgcgcgag
       61 gccggccggc tcatggtcgg ccggcgccgg ggcgacgccc gcgtggcgct ccaggcacga
      121 cgcgaggaag catgccaggc cctgctcgac cagcagcgga tcgcgtgccg attcccagcc
      181 gcgatgcagc gcgaggtagc cgcgcgcggc ggccgggtcg cggacgatgg gcgggccgaa
      241 gcgaacggtg ccgccgcggg cggcgtcctc gaccaacgcc tcgacgacct ccggccgcgg
      301 gtagcaggcg acgtagcgga atcccggcgc acgcgcgcag ccgtcgtgca cctcgccggg
      361 gttgtagagc gtgaggtcgc ccggcccgac gcggcggcgg cgccccgcga tcgcgatctc
      421 gccctcgccg cggacgacgg ccccgatcac gtactcggca tgcgcatgcg gggcgtagcg
      481 atggcggtgc agcgtcgccg tcaggcagtc gagcccgtgg gcgtcgaaat agcggagctc
      541 gtcgtcggac atgcgggcca tcgtagccac gcacgggcgc cgtggcttga acaaaagtcg
      601 cacgcgcgcc gtgcaggtcc gaccaagcgg cgccgctcgt cgcgccgggc gtcgcaggtc
      661 ccatcgccga cgcgcatgag gaaccctgac gacgcggtcc gggggacccc gctgcgggcg
      721 tcgacgcgac ggggtgcatc ggagacgacg aggcggcatc gctcctggcg gactcctttc
      781 cgggtcgacc actccggcgc cgccgtgcgc ggagccgcgc gcccggggcg atcaggccgc
      841 tgccggcgcg aagtcgatga agccgacgac ctcggcgagg cggccctggg catcgaggcg
      901 ggcgatgtcc gtgccgccgg cgatcggctc gccaccggcc ggaccgagcg accaggagaa
      961 tcgcgcgaac tcgccgtgcc cgtcgggcgc gccgcgcagg gagaacgcga gcccggcgaa
     1021 ctgcgcgcgc gccgcctcga tcagcgtgcc gatgtgggcc ggcccggtgc ccgacatcat
     1081 cgggtcggca tagcggccgt cgggcgtcca cagcgccgcg agcagctggc tgcggcgcgc
     1141 cgcgtcgggc tcgttccagg tcgcgaggta gcggtcggcg agggtgcggg gatcggtcat
     1201 ggcgaggctc cttggagggg tgatcgcgac attgcgatgg gggcatcgtc gtcgcgggcg
     1261 cgcggcgggt cgattacccg ggaggtcatg gacgctcgca ccccgctcgg ataccgtgcc
     1321 gggcatgaac ctccacgcct catccaccgc cggcgtcggc gagctcgtgc gcgagtggcg
     1381 ccagcgccgg cgcatgagcc agctcgagct ggccggcgtc gcggacatct ccgcgcgcca
     1441 cctgagcttc gtcgagacgg gtcgggcctc gcccagccgc agcatgctga tccgcctggc
     1501 cgagcgcctg gacgtcccgc tgcgcgcccg caaccagatg ctgcatgcgg cgggctacgc
     1561 cgcgctctac gccgagcgtc cgctggacgc gcccgagatg accgctgccc gccagaccgt
     1621 ggaagccgtg ctgacggcgc acatgccgtt cccggcgctc gccgtcgacc gccactggca
     1681 gctcgtcgcc gccaacgagg cggtcggcgc gctgctggcg ggcatcgatg ccgagctgct
     1741 cgcgccgccc ctgaacgtgc tgcgcctgag cctgcacccg cgcgggctgg cgccgcgcat
     1801 cgccaacctc ggccagtggc gcgcccacct gctcgagcgc ctgcagcagc aggtcgaccg
     1861 ctccggcgac cggcggctcg tcgcgctgca cgcggagctc gtcgagctct cgccacccgg
     1921 tccgcacgac ctccacgacc acgccgcgcc cgagcccggc gccgtcgccg tgccgctgcg
     1981 cctgcgcgtc gacgacgacg agcccctggc cttcctcagc acgaccaccg tcttcggcac
     2041 gccggtggac gtcacgctgt cggagctggc gctcgaatgc ttctacccgg ccgacgatcg
     2101 cacgaggcgg cacctgcagg gcgcgcgcgg ctgagcggcg caccgcgcgg gcgcagcgct
     2161 tgccgggcga gccgcatgcc cgtcgtcatc ggcaccgccg gctggaacat cccgcacgat
     2221 gcggccgacg cgttcgatgc gccggatacg ggctcgcacc tggagcgcta cgcgcgcgtg
     2281 ctggccggcg tcgaggtgaa ctcctcgttc caccgacccc accggcgcgc gacctgggcg
     2341 cgctgggccg cgtccacgcc gcccggcttc cgcttctcgg ccaagctgcc gcgcgccatg
     2401 acccacgacg cacggctgcg cgtggacgac gcggccctcg acgaattcct ggcgcaggcc
     2461 ggcggcctcg gcgacaagct ggccgtgctg ctggtgcaat tgccgccgtc gctcgcgttc
     2521 gagtgcgacg tcgcgctcga cttcttcgcg cgcctgcgcg cacgcaccgg cgcggccatc
     2581 gcctgcgagc cgcgccatcg cagctggttc gaggccggcg cggacgcggc
//
LOCUS       DASVLN010000632         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_408497, whole genome shotgun sequence.
ACCESSION   DASVLN010000632 DASVLN010000000
VERSION     DASVLN010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408497"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1962
                     /locus_tag="VF453_15100"
     CDS             <1..1962
                     /locus_tag="VF453_15100"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX7689040.1"
                     /translation="LAVARTRLHGRALGVLAGAVVAWLALGGWIFWNTNVLNTYTTQP
                     ALDRRAADMEKALLPFESVPQPRVAELALGVQLWPRQARAVTRGRYTIVNRGTQPIAT
                     LHLQWAAGLRLDAIDLPGATVQQDWPRFHYRVYRLAVPLQPGERRVLGFTTTLEERGF
                     PNDRPLTDIVANGTFLDDSMIAPTIGFSRGELLKDRFKRRKYGLPPDLPLPARSDDRA
                     RAFNAFVRDSDWVDTDVTVTTDADQTPVAPGRIVADTGLRADPDARRTVRFRSDAPIL
                     RAFSIQSARYDVRRATWHAASGRDVDLAVYYTPGHEYNVDRMLDRMRTALALDSERFG
                     PYPFAQARIVEFPAYREFAQSFANTIPFSEAIGFVQRWSGDPTKIDVASYVTAHEIAH
                     QWWGHQLVPAQQEGAAMLTESFAQYSAMLAMEKVYGPDAVRRFLRWELNRYLRGRGSE
                     VQDERPLARVQDGQQYVYYNKGSLAMYWASEALGGDVMDRALRRMLDAHAFKGPPYPN
                     TADFLAVLRQEAGPGHDTLIDDLFERITLLDLSASNAVARKRADGRWVVAFDVDAHKF
                     YADGSGRQSEAPMDEKVEIGVFTAEPGGRGFTAADKLLLQQQDIATGRQHVEVVVDRA
                     PAWVGVDPYNKRIDRNAEDNLARVTLAGS"
BASE COUNT          302 a          813 c          738 g          276 t
ORIGIN      
        1 ctggccgtcg cgcgcacgcg cctgcacggc cgcgcgctgg gggtgctggc gggcgcggtc
       61 gtcgcgtggc tggcgctcgg cggctggatc ttctggaaca ccaacgtcct caacacctac
      121 acgacgcagc cggcgctcga ccgccgggcc gccgacatgg agaaggcgct gctgcccttc
      181 gagtccgtcc cgcagccgcg cgtggccgag ctcgcgctgg gcgtgcagct gtggccgcgc
      241 caggcgcgcg ccgtcacccg cgggcgctac acgatcgtca accgcggcac gcagcccatc
      301 gccacgctgc acctgcaatg ggccgcgggc ctgcggctgg acgcgatcga cctgcccggc
      361 gcgacggtgc agcaggactg gccgcgcttc cactaccgcg tctaccggct cgccgtgccg
      421 ctgcagccgg gcgagcgccg cgtcctcggc ttcacgacga cgctggagga gcgcggcttt
      481 cccaacgacc ggccgctcac ggacatcgtc gccaacggca ccttcctcga cgactcgatg
      541 atcgcgccga cgatcggctt ctcgcgcggc gagctgctga aggatcgctt caagcgccgc
      601 aagtacggcc tgccgcccga tctgccgctg cccgcgcgct cggacgaccg cgcccgcgcc
      661 ttcaacgcgt tcgtgcgcga cagcgactgg gtcgacaccg acgtcaccgt gaccaccgac
      721 gccgaccaga cgccggtcgc gccgggccgc atcgtcgccg acaccgggct gcgcgcggac
      781 cccgacgcgc gccgcaccgt gcgcttccgc agcgacgcgc cgatcctgcg ggcgttctcg
      841 atccagtcgg cgcgctacga cgtgcgccgc gccacctggc acgcggcgag cggccgcgac
      901 gtcgacctcg cggtctacta cacgccgggc catgaataca acgtcgaccg catgctcgac
      961 cgcatgcgca ccgcgctggc gctggacagc gagcgcttcg gcccctaccc gttcgcgcag
     1021 gcccgcatcg tggagttccc ggcctaccgc gaattcgcgc agtccttcgc caacacgatc
     1081 cccttctccg aggccatcgg cttcgtgcag cgctggagcg gcgacccgac gaagatcgac
     1141 gtcgcgagct acgtgaccgc gcacgagatc gcgcaccagt ggtggggcca ccagctcgtg
     1201 ccggcgcagc aggagggcgc ggccatgctg accgagtcct tcgcgcagta ttccgccatg
     1261 ctcgcgatgg agaaggtcta cgggcccgac gcggtgcgcc gcttcctgcg ctgggaactc
     1321 aaccgctacc tgcgcggccg cggcagcgag gtgcaggacg agcgcccgct cgcgcgcgtg
     1381 caggacggcc agcagtacgt ctactacaac aagggctcgc tggcgatgta ctgggccagc
     1441 gaggcgctcg gcggggacgt gatggaccgc gcgctgcgcc gcatgctcga cgcgcacgcc
     1501 ttcaagggcc cgccgtaccc gaacaccgcc gatttcctcg ccgtcctgcg ccaggaggcc
     1561 ggaccgggcc acgacacgct gatcgacgac ctgttcgagc gcatcacgct gctggacctg
     1621 agcgcctcga acgccgtcgc ccgcaagcgc gccgacggcc gctgggtcgt cgccttcgac
     1681 gtcgacgcgc acaagttcta cgccgacggc agcggccgcc agtccgaggc gccgatggac
     1741 gagaaggtcg agatcggcgt cttcaccgcc gagccgggcg ggcgcggctt cacggcggcc
     1801 gacaagctcc tgctgcagca gcaggacatc gcgacgggcc gccagcacgt cgaggtcgtg
     1861 gtcgaccgcg cgcccgcgtg ggtgggcgtc gacccgtaca acaagcgcat cgaccgcaat
     1921 gccgaggaca atctggcccg ggtgaccctc gcgggctcct gaggcgcccg cgggcgcccc
     1981 gaagcggccg ggccgccgcc ttgcggatcc ggccgccggc cccacatgcg ggcgttccaa
     2041 ggagccagga tgatcaggtt gtggatgctc agcgccgcgg tgcacgccta cgtggcgctg
     2101 cggctggcgc ccggcgtggc ggcggtggc
//
LOCUS       DASVLN010000633         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_303313, whole genome shotgun sequence.
ACCESSION   DASVLN010000633 DASVLN010000000
VERSION     DASVLN010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303313"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VF453_15105"
     CDS             <1..495
                     /locus_tag="VF453_15105"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7689041.1"
                     /translation="IASVTSALAADTFRLNEVLRAILSTMHRALGFRCVVFGLRDPKS
                     GVITGRFGLGDAAPALSAQLRVDVNPNGPIDLLSVACKKAADTLISDAAADNVRSRLP
                     TWLQREAGARCFVLLPLTMKSAPFALIYADCATPGGIALGDRELSLLRTLRNQAVMAF
                     KTAA"
     gene            637..2175
                     /locus_tag="VF453_15110"
     CDS             637..2175
                     /locus_tag="VF453_15110"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7689042.1"
                     /translation="MFSWFDNLRIGAKLIVVLSAVLALTGLVGAISIVQLDRLSGDAQ
                     QLAGSALPRVRVVAGIRTTVLELRVLQYAHMLSDSEQEQAQLKDQIKGLAARVADLRR
                     QYEPMIASPDERSAYEAFGKSWDGYVQRNEQVLQLTGDFGMQAMGGDYRKAFDATTGS
                     LAAILKVNDRGIDELSRATQSTAAQTRAVICGAVAAAVALGIALAMVMARRIAGSLAV
                     AAARARDVAKGDLTRAIPAGGHDEVGRLLSALGEMQDGLRSLVSTVRSGVESVASSSA
                     EIATGNMNLSTRTEQQSGNLQQTVSAIHQMASHLRRSMESAQQANQLARSATEVAQRG
                     GGAVQQVVHTMGDISDASRRITDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAV
                     VAGEVRALAQRSAQAAHEIKGLIGASAEKVELGARLVQDAGHTMEEIVGSVRRVGDII
                     GEISSAAAEQTTGIDQINDAVTQLDAMTQQNAALVEQSAAAAESLREQGSRLNVAVAR
                     FHLA"
     gene            2316..2876
                     /locus_tag="VF453_15115"
     CDS             2316..2876
                     /locus_tag="VF453_15115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7689043.1"
                     /translation="MNDEAAITLRAVARASDVQALRTLDASHLARSVIEVEASESGFV
                     LREVACGLPFTKTYDADGIARDFEHADLRCIAEDASGRMLGVAIACRESWNRSVLVTG
                     LFVVSTHRGLGLGRRLLTDLERQADAGDARCLLVETQNTNVAAVRFYRALGFALCGVN
                     VALYDPATVRAGECALYFMRPLPSRA"
     gene            complement(2986..3318)
                     /locus_tag="VF453_15120"
     CDS             complement(2986..3318)
                     /locus_tag="VF453_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7689044.1"
                     /translation="MGTLDPVVPLPRAAIKAPKAATAEDCHALEQLPNVGPAMAADLR
                     LLGIHTPQALKGRDGLQLYRALCEATGQRHDPCVLDTLLAVVDFMHGAPPAPWWAYTK
                     QRKALGAL"
     gene            complement(3330..4010)
                     /gene="lexA"
                     /locus_tag="VF453_15125"
     CDS             complement(3330..4010)
                     /gene="lexA"
                     /locus_tag="VF453_15125"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX7689045.1"
                     /translation="MDVESPKLTARQQQILDLVQGAIERTGAPPTRAEIANELGFKSA
                     NAAEEHLQALARKGVIELVGGTSRGIRLKSDTLRALNEARGKQFSLPLPSLSQLTLPL
                     VGRVAAGSPILAQEHVEQTYLLEASMFPRRPDYLLKVKGMSMRDAGILDGDLLAVQKS
                     REARNGQIVVARLGDEVTVKRFRRTRTTIELLPENPDFQPIVVPFGDVDFELEGIAVG
                     LVRNNMFN"
     gene            4098..>4645
                     /locus_tag="VF453_15130"
     CDS             4098..>4645
                     /locus_tag="VF453_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX7689046.1"
                     /translation="MQRSLPLVVILATGGTIAGSAATATDTTGYRAGALGVEALIAAV
                     PALAAHRLESEAVAAIDSKDMDIETWQRLAARVAHHLARPEVAGVVVTHGTDTLEETA
                     YFLHRVLAPAKPVVLTAAMRPASALSPDGPANLLDAVYVAGTPGARGVCLAFGGAVWH
                     PVGLRKVHTLRTAAFDAGDAGP"
BASE COUNT          646 a         1657 c         1683 g          659 t
ORIGIN      
        1 atcgccagcg tcaccagcgc gcttgcggcc gacacgttcc gcctcaacga ggtcctgcgc
       61 gcgatcctgt cgacgatgca tcgcgcgctc ggcttccgct gcgtcgtgtt cggcctgcgc
      121 gatccgaagt ccggcgtcat caccggccgc ttcggcctcg gcgacgcggc gcccgcgctg
      181 tccgcgcagc tgcgcgtgga cgtcaacccg aacgggccga tcgacctgct cagcgtcgcc
      241 tgcaagaagg cggcggacac gctgatctcc gacgccgccg cggacaacgt ccgcagccgc
      301 ctcccgacct ggcttcagcg cgaggccggc gcgcgctgct tcgtgctgct gccgctgacg
      361 atgaagagcg cgccgttcgc cctgatctac gccgactgtg cgacgcccgg cggcatcgcg
      421 ctgggcgacc gcgagctgag cctgctgcgc acgctgcgca accaggcggt gatggcgttc
      481 aagactgccg cctgaggcgg acctgcggag ggcgacgcct caaggatcgc cggtccccgc
      541 cgaaatgcag cgaacggatt cccgcgcgtg ccgtgtgcgc gggagcctcc acgacgacga
      601 cgcggcccga ccgactgaga gaccgaagag gtgtccatgt tctcctggtt cgacaacctg
      661 cgcatcggcg ccaagctgat cgtcgtcctg tccgcggtgc tcgcgctcac cggcctggtc
      721 ggtgcgatct ccatcgtcca gctcgatcgc ctgagcggcg acgcgcagca gctcgccggc
      781 tccgcgctgc cccgcgtgcg ggtggtggcg ggcatccgca cgacggtgct ggagctgcgc
      841 gtgctgcagt acgcgcacat gctgtcggac tccgagcagg agcaggcgca gctcaaggac
      901 cagatcaagg gcctggcggc gcgcgtggcc gacctgcgcc gccagtacga gccgatgatc
      961 gcctcgcccg acgagcgctc ggcgtacgag gccttcggca agtcctggga cggctacgtg
     1021 cagcgcaacg agcaggtgct gcagctgacc ggcgacttcg gcatgcaggc catgggcggc
     1081 gactaccgca aggccttcga tgcgacgacc ggcagcctcg cggcgatcct gaaagtcaac
     1141 gaccgcggca tcgacgagct ctcgcgcgcg acgcagtcga cggcggcgca gacccgggcc
     1201 gtcatctgcg gcgcggtcgc ggcggcggtg gccctgggca tcgcgctggc aatggtgatg
     1261 gcgcgccgca tcgccggctc gctggcggtg gccgcggcgc gcgcccgcga cgtggccaag
     1321 ggcgatctca cgcgcgccat cccggccggc ggccacgacg aggtgggccg gctgctgtcg
     1381 gcgctcggcg agatgcagga cggcctgcgt tcgctcgtga gcaccgtgcg ctcgggcgtg
     1441 gagtcggtgg cctcgtcctc ggccgagatc gccaccggca acatgaacct gtccacgcgc
     1501 actgagcagc agtcgggcaa cctgcagcag acggtctcgg cgatccacca gatggcatcg
     1561 cacctgcgtc gcagcatgga atcggcgcag caggccaacc agctcgcgcg cagcgccacc
     1621 gaggtcgccc agcgcggcgg cggcgcggtg caacaggtcg tgcacacgat gggcgacatc
     1681 agcgatgcgt cgcgccgcat caccgacatc atcggcgtga tcgacggcat cgccttccag
     1741 accaacatcc tggcgctgaa cgccgcggtc gaggccgcgc gggcgggcga acaggggcgc
     1801 ggcttcgcgg tcgtcgccgg cgaggtgcgc gcgctggccc agcgcagcgc gcaggcggcc
     1861 cacgagatca agggcctcat cggcgcctcg gcggagaagg tggagctcgg cgcgcgcctg
     1921 gtgcaggacg ccggccacac gatggaggag atcgtcggct cggtgcgtcg ggtcggcgac
     1981 atcatcggcg agatcagcag cgcggccgcc gagcagacca ccggcatcga ccagatcaac
     2041 gacgcggtga cgcagctcga cgcgatgacg cagcagaacg ccgcgctcgt cgaacagagc
     2101 gccgcggccg ccgagagcct gcgcgagcag ggctcgcggc tgaacgtcgc ggtggcgcgc
     2161 ttccacctgg cctgagcgac gcgccgggcc gcggcgtgga aaaatcaggc catgctggcc
     2221 tgcctcttgc cgctgtcacc gacccgggcg tcggctcggc tgccgacttc gtcccgcggt
     2281 gcgccgttcg aggacgtccg ccgggcgcct gacgcatgaa cgacgaagcc gcgatcacgc
     2341 tgcgcgccgt cgcgcgggct tcggacgtgc aggcgctgcg cacgctcgac gcgagccacc
     2401 tggcgcgctc ggtgatcgag gtggaggcca gcgaatccgg cttcgtgctg cgtgaagtcg
     2461 cgtgcggcct gccgttcacg aagacctacg acgccgacgg catcgcccgc gacttcgagc
     2521 acgccgacct gcgctgcatc gccgaggacg cgtcgggcag gatgctgggc gtcgcgatcg
     2581 cgtgccgcga gtcgtggaac cgcagcgtcc tcgtcaccgg tttgttcgtc gtgtcgacgc
     2641 atcgcgggct cggcctgggg cgccggttgc tgaccgacct cgagcgccag gcggacgcgg
     2701 gcgatgcgcg ctgcctgctg gtggagacgc agaacacgaa cgtcgcagcc gtgcgcttct
     2761 atcgcgccct cggcttcgcg ctgtgcggcg tgaacgtcgc cctctacgac cccgcgacgg
     2821 tgcgtgcagg cgaatgcgcg ctgtacttca tgcggcccct gccgtcgcga gcgtagcggt
     2881 cgtttcgtcc gtgtgccgcg ttgcccgccg tgccagcgga atcggtcggg cgctaccgcg
     2941 gggcggaacc ggcctcgacc cgccggccgc cagggcctcg accggtcaca gcgcgcccag
     3001 ggccttgcgc tgcttcgtgt aggcccacca gggcgcgggc ggcgcaccat gcatgaagtc
     3061 gacgaccgcc agcagcgtgt cgagcacgca ggggtcgtgg cgctggccgg tggcctcgca
     3121 cagcgcgcga tagagctgca ggccatcacg gcccttgagc gcctgcggcg tatggatgcc
     3181 cagcaggcgc agatcggccg ccatcgcggg gccgacgttg ggcaactgct ccagcgcgtg
     3241 gcagtcctcg gccgtcgccg ccttgggcgc cttgatcgcg gcgcgcggca gcgggacgac
     3301 cggatccagc gtgcccaccg gcggcctcgt cagttgaaca tgttgttgcg caccagcccc
     3361 accgcgatgc cctcgagctc gaagtcgacg tcgccgaagg gcacgacgat gggctggaag
     3421 tccgggttct cgggcagcag ctcgatggtg gtgcgagtgc ggcggaagcg cttgaccgtg
     3481 acctcgtcgc ccaggcgcgc gaccacgatc tggccgttgc gggcttcccg gctcttctgc
     3541 accgccagca ggtcgccgtc caggatgccg gcgtcgcgca tgctcatgcc tttcaccttg
     3601 agcaggtagt cgggccggcg cggaaacatc gaggcttcca gcaggtaggt ctgctcgacg
     3661 tgctcctgcg cgaggatggg cgagccggcc gccacgcgcc cgaccagcgg cagcgtcagc
     3721 tgcgagaggc tgggcagcgg cagcgagaat tgcttgccgc gcgcctcgtt gagcgcccgc
     3781 agtgtgtcag acttcagccg gatgccgcgg gacgtcccgc cgacgagctc gatcacgccc
     3841 ttgcgggcga gggcctgcag gtgctcctcc gcggcgttgg ccgacttgaa gcccagctcg
     3901 ttggcgatct cggcccgggt ggggggcgcg ccggtgcgct cgatcgcgcc ctgcaccagg
     3961 tcgaggatct gctgctggcg ggcggtgagc ttgggcgatt cgacatccat ggcgtccggg
     4021 tccgcaggag actcgcgtcc cgcagcgggc atctggttgt tcatacagtt ctgtattttt
     4081 atccaaggtt tcgaagaatg caaaggtcct tgccgctcgt cgtgatcctg gccaccggcg
     4141 gcaccatcgc cggcagcgcc gcgaccgcga cggacaccac cggctaccgc gcgggcgcac
     4201 tgggcgtgga ggcgctcatc gccgccgtgc cggcgctcgc cgcccaccgg ctcgagtcgg
     4261 aggcggtcgc cgcgatcgac agcaaggaca tggacatcga gacctggcag cgcctggccg
     4321 cgcgtgtcgc gcaccacctc gcgcggccgg aggtcgccgg cgtcgtcgtc acgcacggca
     4381 cggacacgct ggaggagacc gcgtacttcc tgcaccgcgt gctcgcgccg gccaagccgg
     4441 tcgtgctgac ggcggcgatg cgtcccgcga gcgcactctc gcccgacggc ccggccaacc
     4501 tgctcgacgc cgtctacgtg gccggcacgc cgggcgcgcg cggggtgtgc ctcgcgttcg
     4561 gcggcgcggt gtggcacccg gtcgggctgc gcaaggtgca cacgctgcgc accgcggcct
     4621 tcgacgccgg cgacgccggg cccat
//
LOCUS       DASVLN010000634         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_408508, whole genome shotgun sequence.
ACCESSION   DASVLN010000634 DASVLN010000000
VERSION     DASVLN010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408508"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2097
                     /locus_tag="VF453_15135"
     CDS             <1..>2097
                     /locus_tag="VF453_15135"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689047.1"
                     /translation="RASPVRETLRFTLTAIACSLVGATVGEGTLCWLGLVPRPELFFG
                     WMTWWLGDACGMLVVTPGLLLLADPRLRDDRLTLQAFPLMCLGLGLTAFSTFAVGLVD
                     RDAHIERFRADTGRLAMTLQGHVELALRDLEALQHAFYKADLDRDEFHALCAPLLARS
                     PWQSTFEWLPRVAAADRDPFETSPSGLGGISIREVDPSGAVVRAGSRAEYFPVAWTEP
                     EAGRETLRGLDHAADAERGTALARARGTGATTSSPPLSTIAYSPDERVVQTLYIAVGG
                     TGERLGEPFEPARVRGLVAATMDLASMLQASIAQMGTSDATALLYDPDAPHAASLLWR
                     GGGAVETVVPVERDRLLAAMTGGVSQRLWIRVGDRRWGLAAQPTWASTMPHPSWLQGA
                     VLCSGLAFTGLLTAFLVVRRRRDEALESARDQLEQQVRARTQDLAATNGRLLEEIAGH
                     RRTEQLLQDARRNAEAANRAKSQFLANMSHEIRTPLNAVLGYTQLLIEDKRQPSDSRE
                     RLRIIHGAGQRLLGLINDVLDLAKIEAGGLQVHVEPIDLRRELEEIGTLVAPRVQAKG
                     LALRVDIDLDSDAAVLADRAKFGQIVLNLLGNALKFTDAGTIALRAWRSGGDTIVEVE
                     DTGPGMDAQELASVFTAFRQGAAGVDKGGTGLGLNLSRHLATALGGELSITSTPGRGT
                     RVCLRLPMAEAGVAAVA"
BASE COUNT          268 a          785 c          766 g          278 t
ORIGIN      
        1 gcgtgccagc ccggtgcgcg agacgctgcg cttcacgctg acggccatcg cctgcagcct
       61 cgtcggcgcg acggtcgggg agggcacgct gtgctggctc gggctggtgc cgcggccgga
      121 gctgttcttc ggctggatga cctggtggct gggcgacgcc tgcggcatgc tggtggtgac
      181 gccgggcttg ctgctgctgg ccgacccgcg cctgcgcgac gaccgcctga cgctccaggc
      241 ctttccgctg atgtgcctgg gcctgggcct gactgcgttc agcaccttcg cggtgggcct
      301 cgtcgatcgc gatgcccaca tcgagcgctt ccgtgccgac accggtcggc tcgcgatgac
      361 gctgcagggc cacgtggagc tcgcgctgcg cgacctcgag gcgctgcagc acgcgttcta
      421 caaggccgac ctcgaccgcg acgaattcca cgccctgtgc gcgccgctgc tcgcgcgcag
      481 cccgtggcaa tcgaccttcg agtggctgcc ccgcgtcgca gccgccgacc gcgacccgtt
      541 cgagacctcg ccgtcggggc tgggcggcat ctcgatccgc gaggtcgatc cgagcggcgc
      601 cgtcgtgcgc gccggctcgc gcgccgagta tttcccggtc gcgtggaccg agcccgaggc
      661 cggccgcgag acgctgcgcg ggctcgacca cgcggccgac gccgagcgcg gcacggcgct
      721 cgcgcgggcc cgcgggacgg gcgcgacgac gtcctcgccg ccgctctcga cgatcgccta
      781 cagtcccgac gagcgcgtcg tgcagacgct ctacatcgcg gtcggcggca ccggcgaacg
      841 cctgggcgag cccttcgagc cggcacgcgt gcgcggcctg gtggccgcga cgatggacct
      901 cgcgtcgatg ctgcaggcct ccatcgcgca gatgggcacg agcgacgcga cggccctgct
      961 ttacgacccc gatgcgccgc acgccgccag cctgctgtgg cgcggcggcg gcgcggtcga
     1021 gacggtggtg ccggtggagc gcgaccgcct gctcgcggcg atgaccggcg gggtgtcgca
     1081 gcggctgtgg atccgcgtcg gcgaccgccg ctggggcctg gccgcgcagc ccacgtgggc
     1141 cagcacgatg ccgcatccga gctggctgca gggcgcggtg ctgtgcagcg ggctcgcgtt
     1201 caccggactg ctcacggcct tcctcgtcgt gcgccggcgc cgcgacgagg cgctcgaatc
     1261 cgcccgcgac cagctcgagc agcaggtacg cgcccgcacg caggatctcg cggccaccaa
     1321 cgggcgtctc ctcgaggaga tcgccggcca ccgccgcacc gagcagctgc tgcaggacgc
     1381 gcgccgcaac gccgaggcgg ccaaccgcgc gaagtcccag ttcctcgcca acatgagcca
     1441 cgagatccgc acgccgctga acgccgtcct cggctacacg caactgctga tcgaggacaa
     1501 gcgccagccg tccgacagcc gcgagcgcct gcgcatcatc cacggcgccg gccagcgcct
     1561 gctggggctg atcaacgacg tgctcgacct ggccaagatc gaggccggcg gcctgcaggt
     1621 gcatgtcgag ccgatcgacc tgcggcgcga gctcgaggag atcggcacgc tggtcgcgcc
     1681 gcgcgtccag gccaagggcc tcgcgctgcg cgtggacatc gacctcgact cggacgccgc
     1741 ggtgctggcc gaccgcgcca agttcggcca gatcgtgctg aacctgctgg gcaacgcgct
     1801 gaagttcacc gacgccggca ccatcgcgct gcgcgcgtgg cggtcgggcg gcgacacgat
     1861 cgtcgaggtc gaggataccg gcccgggcat ggacgcgcag gagctcgcgt cggtcttcac
     1921 cgcattccgc cagggcgcgg ccggcgtcga caagggcggc accggactcg gcctgaacct
     1981 gtcgcggcac ctggcgaccg cgctcggagg cgagctctcg atcacgagca ccccgggccg
     2041 cggcacccgc gtctgcctgc gcctgccgat ggccgaggcc ggcgtggcgg cggtggc
//
LOCUS       DASVLN010000635         4077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_87261, whole genome shotgun sequence.
ACCESSION   DASVLN010000635 DASVLN010000000
VERSION     DASVLN010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87261"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VF453_15140"
     CDS             <1..621
                     /locus_tag="VF453_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7689048.1"
                     /translation="HFGRATLGEALHAVVVPAVDVARSHAKIFKSPRAGRAERDKGLA
                     VVDVAMAACAAPAYFPCARAGGRLYADGGLFAVAPDQVAMHEAEHFDRIAPARMRMLS
                     IGTAARHYRPARAIDPQAGAVGWLADGRLMLTLVSAQQQHVAAMMADRLGDRYLRVDA
                     DWVPQAGLGIDVATAQAREYLKARARRTVEALPRAAIGPFLALRDA"
     gene            706..2439
                     /locus_tag="VF453_15145"
     CDS             706..2439
                     /locus_tag="VF453_15145"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7689049.1"
                     /translation="MNPQNRPIAEAFVQIANLLELQRADPVRVRAYRKASQAIGALPD
                     SVRALVHQGRALDDVTALGEPLAGAAVELAETGSCALLQRLHAEIPPDFNELLELPGL
                     GPQRVRTLHEELGVRTIRQLHDAAAAGRLQSLAGFGPRLEQQLLRATAASLSRVRRLR
                     RDQARPVARAMSDWLLGIDGVRRVEVAGCLRRECESVGDVNLVAQCSDDRKIVSALCA
                     HPEVREVPYAGRARAGVVLADGARVDLQVSGEAEFGAHWLARTGSAAHVRALSLRARH
                     ARLTLDETGLRRDGRQVDDGSEASIYRQLDLAFIPPELREDAGEIEAAAQGTLPALVR
                     RDDLVGDLRVRTRVGAAETPLAAIVEDARARGLSYIAVADPARRLVSDRASDLDRLAA
                     QIDEIDRLNEALAGRFVVLAGVHAGILEDGRLNVPDAILGRVDLVFASLEVALDLTRT
                     RQTDRLLRALDNRFLSILAAPTGRRVEERRGAAFDLARVLRKARERGCFVELDGRPDR
                     LDLDAAGCRLAGELGVLVAVDSCACDAAGFDALDDGVGQARRGGLEARQVLNTRPVEE
                     LRRLLAPTMSH"
     gene            2525..3595
                     /locus_tag="VF453_15150"
     CDS             2525..3595
                     /locus_tag="VF453_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7689050.1"
                     /translation="MSDATDHPPSPAPRCLVTGGSGFLGINLVRHLLGLGWTLRTLDI
                     APFDYPERHRIDAVLGDIRDPAMVARAVDGMDAVVHCAAALPLAPDADILSTGVLGTG
                     TLLSAARRSRVGRFVFISSTAVYGVPDERPIPETHRLVGVGAYGRSKIEAERLCERAR
                     ADGLCTPILRPKTFVGPERLGVFELLYDWAAQGRGFPVLGRGDNPYQLLDVRDLCEVI
                     ERCLTGPRDAVDDCFNVGAASFGTMADNVQCVLDRAGFGARVAHLPARPAVWLLRALR
                     AVGLSPLYPWIYETAGHDSEVAIDKLRSRLGYSPPHSNAAALAANYDWYLAHRREFAR
                     RRGVTHRLPWKRGALGLAKVFF"
     gene            3595..>4077
                     /locus_tag="VF453_15155"
     CDS             3595..>4077
                     /locus_tag="VF453_15155"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEX7689051.1"
                     /translation="MLAPEALGQAPPHCSPAAAPARADDPPLVVDLDGTLLRTDSLVE
                     SLFVLAKRRPASLLALPCALRRGRAAFKRWVAVRARPELETLPFDEPMMDLLRAEKRR
                     GRRLVLATGADQLLAQAIADRLDLFDEVLASDGRRNLAGQVKAERLVAAYGARGFDYA
                     G"
BASE COUNT          463 a         1577 c         1506 g          531 t
ORIGIN      
        1 cacttcggcc gcgcgacgct gggcgaggcg ctccacgccg tggtggtgcc ggcggtcgac
       61 gtcgcgcgca gccacgcgaa gatcttcaag tcgccgcgcg ccgggcgcgc cgagcgcgac
      121 aagggcctcg cggtcgtgga cgtcgcgatg gccgcctgcg ccgcgccggc ctactttccc
      181 tgcgcgcgcg ccggcgggcg cctgtatgcc gacggcgggc tcttcgcggt ggcgcccgac
      241 caggtcgcga tgcacgaggc cgagcacttc gaccgcatcg cgcccgcccg gatgcgcatg
      301 ctgtcgatcg gcacggcggc gcgccactac cggccggcgc gcgcgatcga cccgcaggcc
      361 ggcgcggtcg gctggctcgc ggacggccgc ctgatgctca cgctcgtctc ggcgcagcag
      421 cagcatgtcg ccgccatgat ggccgaccgg ctcggcgacc gctacctgcg ggtcgacgcg
      481 gactgggtgc cgcaggccgg gctgggcatc gacgtcgcca ccgcgcaggc gcgcgagtac
      541 ctgaaggcgc gggcgcggcg caccgtcgag gcgctgccgc gggccgccat cgggcccttc
      601 ctcgcgcttc gcgatgcgta gcgggcggcg gcgagcgcag caggacggtg ccctgcgttg
      661 agcgcccgca atcgccgcgc gccggcgcgc cctagggtga cggcgatgaa tccgcagaac
      721 cgcccgatcg ccgaagcgtt cgtccagatc gccaacctcc tcgagctgca gcgcgccgac
      781 ccggtgcgcg tgcgcgccta tcgcaaggcg tcgcaggcga tcggcgcatt gccggacagc
      841 gtgcgggcgc tcgtgcacca gggacgcgcg ctggacgacg tcactgcgct cggcgagccc
      901 ctggccggcg ccgcggtcga gctcgccgag acgggctcgt gcgcgctgct gcaacgcctg
      961 cacgcggaga tcccgcccga cttcaacgag ctgctcgagc tgcccggcct gggcccgcag
     1021 cgcgtgcgca cgctgcacga ggagctgggc gtgcggacga tccgccagct ccatgacgcg
     1081 gcggcggcag gtcgtctgca gtcgctcgcg ggcttcgggc cgcggctcga gcagcagctc
     1141 ctgcgcgcga ccgccgccag cctctcccgc gtccggcgcc tgcgccgcga ccaggcgagg
     1201 ccggtggcgc gcgcgatgag cgactggctc ctcggcatcg acggcgtccg ccgggtcgag
     1261 gtcgccggct gcctgcgccg cgagtgcgag agcgtcggcg acgtgaacct cgtcgcgcag
     1321 tgcagcgacg accgcaagat cgtgtcggcg ctgtgcgcgc atcccgaggt ccgcgaggtg
     1381 ccgtatgccg gccgggcccg ggcgggcgtc gtgctggccg acggcgcccg cgtcgacctg
     1441 caggtctccg gcgaggccga gttcggcgcc cactggctcg cgcgcacggg atccgcggcg
     1501 cacgtccgcg cgctctcgct gcgcgcccgc cacgcccgcc tgacgctgga cgagaccggg
     1561 ctgcgccgcg acgggcgcca ggtcgacgac ggctccgagg cctcgatcta ccggcagctc
     1621 gacctcgcgt tcatcccgcc cgagctgcgc gaggacgccg gcgagatcga ggcggccgcc
     1681 cagggcaccc tgccggcgtt ggtgcggcgc gacgacctgg tcggggacct gcgcgtgcgc
     1741 acccgcgtcg gcgcggcgga gactcccctg gcggcgatcg tggaggacgc acgcgcgcgc
     1801 gggctgtcct acatcgccgt cgccgacccg gcgcgccgcc tcgtgtccga ccgcgcgagc
     1861 gacctcgacc ggcttgccgc gcagatcgac gagatcgacc gcctcaacga ggccctggcc
     1921 ggacgcttcg tggtgctcgc cggggtgcat gccggcatcc tcgaggacgg ccgcctgaac
     1981 gtccccgacg ccatcctcgg ccgggtcgac ctggtgttcg cgagcctgga ggtcgcgctc
     2041 gacctgacac gcacgcgcca gaccgaccgc ctcctgcgcg cgctggacaa ccgcttcctc
     2101 agcatcctcg ccgcgccgac gggccgtcgg gtggaggaac gccgcggcgc ggccttcgac
     2161 ctcgcgcgcg tgctgcgaaa ggcgcgcgag cgcggctgct tcgtggagct cgacggccgc
     2221 cccgaccgcc tcgacctcga cgccgccggc tgccgcctcg cgggcgagct gggcgtgctc
     2281 gtcgccgtcg attcctgcgc ctgcgacgcc gccggcttcg acgcgctgga cgacggcgtc
     2341 gggcaggcgc gccgaggcgg cctcgaggcc cggcaggtgc tcaacacccg gcccgtcgag
     2401 gagctgcgcc ggctgctcgc cccgacgatg tcgcattgac cgccagggtt gagcaggcgc
     2461 aagccggcca ggcgccggcc ggcggataac ggacgtcgcg cgcccgcgcc ttgcgaccgc
     2521 ccccatgtcc gacgcgaccg atcatccgcc ctcccccgcg ccccgctgcc tggtcaccgg
     2581 cggctcgggc ttcctgggca tcaacctcgt gcggcacctg ctgggcctcg gctggacgct
     2641 gcgcacgctc gacatcgccc cgttcgacta cccggaacgg catcgcatcg acgcggtgct
     2701 cggagacatc cgcgacccgg cgatggtcgc gcgcgccgtg gacggcatgg acgccgtcgt
     2761 ccactgcgcg gcggcgctgc cgctcgcgcc cgacgcggac atcctgtcca ccggcgtgct
     2821 cggcaccggg acgctgctgt cggccgcgcg gcgatcgcgg gtcgggcgct tcgtgttcat
     2881 ctcgtcgacg gcggtctacg gcgtgcccga cgagcggccg atccccgaga cccatcgcct
     2941 cgtcggcgtc ggcgcctacg gccgctcgaa gatcgaggcc gagcgcctgt gcgagcgcgc
     3001 gcgggcggac ggcctgtgca cgccgatcct gcggccgaag accttcgtcg gcccggagcg
     3061 gctcggcgtg ttcgagctgc tgtacgactg ggccgcgcag ggccggggct ttccggtcct
     3121 gggccgcggc gacaacccgt accagctcct cgacgtgcgc gacctgtgcg aggtcatcga
     3181 gcgctgcctg acggggccgc gagacgccgt cgacgactgc ttcaacgtcg gcgcggcgag
     3241 cttcggcacg atggccgaca acgtgcagtg cgtgctcgat cgcgccggct tcggcgcgcg
     3301 cgtcgcgcac ctgccggccc gcccggcagt gtggctgctg agggcgctgc gcgccgtggg
     3361 cctgtcgccg ctgtatccct ggatctacga gacggcgggc cacgacagcg aggtcgccat
     3421 cgacaagctc cgctcgcgcc tcggctactc cccgccgcac tcgaacgcgg ccgcgctggc
     3481 ggccaactac gactggtacc tcgcgcaccg gcgggaattc gcgcggcgcc gcggcgtcac
     3541 ccatcgcctg ccctggaagc gcggggcgct ggggctcgcc aaggtgttct tctgatgctc
     3601 gcgcccgaag cgctcggcca ggcgccgccg cattgcagcc cggcggccgc gcctgcgcgc
     3661 gcggacgatc cgcccctggt ggtggacctg gacggcacgc tgctgcgcac cgattcgctg
     3721 gtcgagtcgc tgttcgtgct cgccaagcgg cgcccggcca gcctgctcgc cctgccttgc
     3781 gcgctgcggc gcggccgcgc ggcgttcaag cgatgggtcg ccgtgcgcgc ccggcccgag
     3841 ctcgagaccc ttccgttcga cgagccgatg atggatctcc tgcgcgccga gaagcgccgc
     3901 ggccgtcgcc tggtcctcgc cacgggcgcc gaccagctgc tcgcccaggc gatcgcggat
     3961 cgcctggacc tcttcgacga ggtgctggcg agcgacggcc ggcgcaacct cgcgggccag
     4021 gtcaaggcgg agcgcctggt cgcggcctac ggcgcgcgcg gcttcgacta cgcgggc
//
LOCUS       DASVLN010000636        10099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_194199, whole genome shotgun sequence.
ACCESSION   DASVLN010000636 DASVLN010000000
VERSION     DASVLN010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194199"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /gene="prpC"
                     /locus_tag="VF453_15160"
     CDS             <1..1105
                     /gene="prpC"
                     /locus_tag="VF453_15160"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEX7689052.1"
                     /translation="SGVVAGNTALCTVGRTGNDLHYRGYDILDVATTCEFEEIAYLLV
                     HGKLPTRAELAGYKSKLKSMRGVPASVKRVLEELPASTHPMDVMRTAVSAIGCALPEK
                     DDHNAPGARDIANRLMASLGSMLLYWYHFAFNGKRIDVETDDDSIGGHFLHLLHGEKP
                     SDLWVRSMHTSLILYAEHEFNASTFTARVIAGTGSDMHSCIAGAIGALRGPKHGGANE
                     VAFEIQKRYDTPDEAEADIRARVEKKEVVIGFGHPVYTVSDPRNKVIKDVARELSAAQ
                     DNTKMFDIAERLESTMWAIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVISRTSGWSA
                     HVIEQRIDNKIIRPSANYTGPEDLAFVPIAQRT"
     gene            1200..3800
                     /gene="acnD"
                     /locus_tag="VF453_15165"
     CDS             1200..3800
                     /gene="acnD"
                     /locus_tag="VF453_15165"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEX7689053.1"
                     /translation="MNAAHRKRLPGTSLDYFDAREAVEAIRPGAWATLPYTARVHAEN
                     LVRRCDPAILGECLAQVAERRRDRDFPWFPARVVCHDILGQTALVDLAGLRDAIAEQG
                     GDPALVNPVVPVQLIVDHSLAVECGGDDPDAFAKNRAIEDRRNEDRFHFIDWTKRAFR
                     NVDVIPPGNGIMHQINLERMSPVVHAQDGVAFPDTLVGTDSHTPHVDALGVIAVGVGG
                     LEAENVMLGRASWMRLPEMVGVELSGKLAAGLTATDLVLALTEFLRKSKVVGAYLEFR
                     GPGAATLTLGDRATISNMAPEYGATAAMFAIDDQTLAYLRLTGRADEQVALVEAYAKA
                     AGLWAADLAHATYDRTLSFDLSTVVRNMAGPSNPHARVATADLAAKGIAGAWTETPGL
                     MPDGAVIIAAITSCTNTSNPRNVIAAGLLARNANRAGLARKPWVKSSLAPGSKAVTLY
                     LEDAGLLPELEKLGFGVVAYACTSCNGMSGALDPKIQQEIVERDLYATAVLSGNRNFD
                     GRIHPYAKQAFLASPPLVVAYAIAGTIRFDIEKDVLGKTADGRDIRLADLWPTDAEVD
                     AILARSVKPEYFRQVYEPMFAIRPADAASVTPLYDWRPQSTYIRRPPYWEGALAGERT
                     LRGMRPLAILPDNITTDHLSPSNAILPDSAAGEYLARMGLPEEDFNSYATHRGDHLTA
                     QRATFANPTLKNEMVRNADGGQRAGSLARVEPEGRVMRMWEAIEAYMARRQPLIIVAG
                     ADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLVGMGVLPLEFLPGTDRRTLGL
                     DGTETYDVVGARTPRATLTLVVHRRDGTRVDVPVTCRLDTAEEVSIYEAGGVLQRFAQ
                     DFLASTKQAA"
     gene            3818..5011
                     /gene="prpF"
                     /locus_tag="VF453_15170"
     CDS             3818..5011
                     /gene="prpF"
                     /locus_tag="VF453_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEX7689054.1"
                     /translation="MSSIPQIRIPATYLRGGTSKGVFFRLDDLPERARVPGPARDALL
                     MRVIGSPDPYGKQIDGMGGATSSTSKTVIVSPSTRAGHDVDYLFGQVAIDKRFVDWSG
                     NCGNLSAAVGPFAIASGLIPAERVPRDGLATVRIWQANIGKTIVAHVPMSGGAVQETG
                     DFELDGVTFPAAEVALEFLDPADEGDGEGGGAMFPTGNVVDRLEVPGVGTFDATLINA
                     GIPTIFLNADAIGYTGAELQDAINGDPKALAMFEAIRAHGAVRMGLIAHVDEAAGRQH
                     TPKVAFVAPPKDYVASSGKAVKAADVDLLVRAMSMGKLHHAMMGTAAVAIGTAAAIPG
                     TLVNLAAGGGTRDAVRFGHPSGTLRVGAEATLVDGAWVVTKALMSRSARVLMEGWVRV
                     PGDAF"
     gene            5064..5924
                     /locus_tag="VF453_15175"
     CDS             5064..5924
                     /locus_tag="VF453_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX7689055.1"
                     /translation="MSTRKTLRALAEARRGVLVPGAFNALSARVIADLGFEAIYVTGA
                     GVTNMWFGMPDQGFMGLHEIADHTARIRDAVELPLIVDADTGFGNALNVRHTVRVLER
                     AGADCIQLEDQVAPKRCGHFTGKEVISTEEAVAKIKAAVDARHDPDFLVMARTDAAAT
                     HGFEAAVERAQRFAEAGADILFVEAVTTADEIRALPRRLGPPQLMNMVIGGRTPIFGA
                     EELGTLGYGLVLYANAALQGAVAGMQKVLTVLRDTKRVDEDPALVAPFKERQRLVGKP
                     EWDALEKKYT"
     gene            complement(5985..7352)
                     /locus_tag="VF453_15180"
     CDS             complement(5985..7352)
                     /locus_tag="VF453_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7689056.1"
                     /translation="MRHLPLHAAALCAALVPAARAADLPTDAQQKLEQQIQQLQQQLD
                     AVKQALQQMQTQAAQRQAAPAPAPVAVAAPAPVSAPGLSPDLSLWGYGELYYTHPVHD
                     ASRTQADLARAVFGIGYRLDDRTSFNSEFEVEHAVASADDRGEFEVEQFYVDHQFTDQ
                     VSGKGGLFLMPFGLLNEHHEPTAFYGVQRNFVETLVIPSTWREGGLALHGTSDLGLSW
                     DAGLTTGLNLSGWNNSPEDPQYRTALELEDNDVAPFQATHQELQLADASHLAQFAALN
                     YRGVPGLLVGGAFFTGDAAMPAVPAGLPQARTTLWEAHARWTPGAADIQAVFAKGTIS
                     HTAAYNIANAGVANPLPSSFLGYYAQAAYTLWQDGDARFSPFVRWEHYDMGASYAGIA
                     PGFSTTPTGLASDGKPWPQPHDRVTTLGANYLVNSHLVLKADYQHFNVNTDFSRVDLG
                     LGVSF"
     gene            complement(7358..8803)
                     /locus_tag="VF453_15185"
     CDS             complement(7358..8803)
                     /locus_tag="VF453_15185"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX7689057.1"
                     /translation="MKSVAGLNFIVRTAGRVRGRAAALLATLALAACGGGGSDSAGPG
                     GTPSDPAAMSAAAQLGQRIFADTALSESGKQSCATCHAKAFAMAADPGASGPDHGLAV
                     PLGGPGMDQPGFRNTPSLLYASYAPAFSFAADGSPSGGFFRDGRAATLADQAALPFTT
                     PFEMANADAAAVVAKLKTRPYLAQFTALYGSDALASPEVALRRMAQALAAFETEAPEF
                     HPFTSKYDYWRAGRAQLTAQELDGLGLFNDPAKGNCAACHPSTSGDGITPPLFTDFSF
                     DNLGVPRNAALPANAGAGAPAYTPADSSDGVQAYYDLGICGPFRDNGGLLVPGLCGQF
                     KVPTLRNVALTAPYFHNGRFATLQQAIGFYVRRDTNPEEWYPTDAAGRVTKFDDLPAA
                     YGGQVVVQAGVAGSDLGYYGNVNTLEIPYDRHIGDAPALSPAEIDDVIAFLCTLSDGY
                     DPAHPDAQAPPAQCVAATRGAAAPSSHPRNP"
     gene            8992..9534
                     /locus_tag="VF453_15190"
     CDS             8992..9534
                     /locus_tag="VF453_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX7689058.1"
                     /translation="MKWSPSGLAPIALAVPAAVAPQLCAAKVFLSVEQAQKDMFGAAT
                     LAATPVVLTAAQQDRLKEVSSVPSPFQGNRVWRASDGGWFVVDEVVGKHELITYAVGL
                     NADGTVRRVEVLEYRESYGGEVAGASWLKQFAGKSAAAPVKVGQDIANISGATLSSRH
                     LADGVHRVLELYASVLRTMG"
     gene            9531..>10099
                     /locus_tag="VF453_15195"
     CDS             9531..>10099
                     /locus_tag="VF453_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX7689059.1"
                     /translation="MSAAPTGLARCRPALGTLVHVALRDDLPHDALLAAVDAAFAAIE
                     AVERAMSFHRPDSDLSRLNAAPAGATLALDPGLVEVLRFSAELHALSDGVFDPGVADR
                     LVAWDVLPRPAAAHPPLAGRATSIRDIEIVDARHVRVLRATCLDLGGVAKGFAVDRTI
                     EALRAHGIAQADVNAGGDLRVLGEPRPVHV"
BASE COUNT         1489 a         3623 c         3572 g         1415 t
ORIGIN      
        1 gtcgggcgtc gtcgccggca acacggccct ttgcaccgtc ggccgcaccg gcaacgacct
       61 gcattatcgc ggctacgaca tcctcgacgt cgcgaccacg tgcgagttcg aggaaatcgc
      121 gtacctcctc gtgcatggca aattgccgac gcgcgccgag cttgccggct acaagtcgaa
      181 gctgaaatcg atgcgcggcg tgccggcttc ggtcaagcgc gtgctcgaag agctgcccgc
      241 gtcgacgcat ccgatggacg tcatgcgcac ggccgtgtcg gcgatcggct gcgcactgcc
      301 cgaaaaggac gatcacaacg cgccgggcgc gcgcgacatt gccaatcggc tcatggcatc
      361 gctcggctcg atgctgctct actggtatca cttcgcgttc aacggcaagc gcatcgacgt
      421 cgagaccgat gacgattcga tcggcgggca tttcctgcat ctcctgcacg gggagaagcc
      481 gagcgatctc tgggtgcgtt cgatgcacac gtccttgatc ctctatgccg agcacgagtt
      541 caacgcatcg acgtttaccg ctcgcgtcat cgccggcacc ggcagcgaca tgcattcgtg
      601 catcgcgggc gcgatcggcg cgctgcgcgg cccgaagcac ggcggcgcga acgaagtcgc
      661 gttcgagatc cagaagcgct acgacacgcc ggacgaggca gaagccgaca tccgcgcgcg
      721 tgtcgagaag aaggaggtcg tcatcggctt cggccatccc gtctacacgg tcagcgatcc
      781 gcgcaacaag gtgatcaagg acgtcgcgcg cgagctatcg gccgcgcagg acaacacgaa
      841 gatgttcgac atcgccgagc gcctcgaatc gacgatgtgg gcgatcaaga aaatgttccc
      901 gaatctcgac tggttcagcg ccgtcagcta ccacatgatg ggcgtgccca ccgcgatgtt
      961 cacgccgctg ttcgtgatct cgcgcacgtc gggctggtcg gcgcacgtga tcgagcagcg
     1021 catcgacaac aagatcattc gcccgagcgc gaactacacc ggcccggaag acctcgcgtt
     1081 cgtgccgatc gcgcaacgca cttaagctgt cttccggcat cgcgggcacc gggctcggtg
     1141 cccgttttcc ttttccgggg tcgcgcgacc ccgccgtccg caaggccaag gccatgacca
     1201 tgaacgccgc ccaccgcaag cgcctgcccg gcacctcgct cgactacttc gacgcccgcg
     1261 aggccgtcga ggcgatccgg ccgggcgcct gggccaccct gccctacacc gcgcgcgtgc
     1321 acgccgagaa cctggtgcgc cgctgcgatc cggccatcct cggcgagtgc ctcgcgcagg
     1381 tcgccgagcg ccggcgcgac cgcgacttcc cgtggttccc ggcgcgcgtg gtctgccacg
     1441 acatcctcgg ccagaccgcg ctggtcgacc tcgccggcct gcgcgacgcg atcgccgagc
     1501 agggcggcga cccggcgctg gtcaacccgg tggtgccggt gcagctgatc gtcgaccact
     1561 cgctggccgt cgagtgcggc ggcgacgacc ccgacgcgtt cgcaaagaac cgcgcgatcg
     1621 aggaccggcg caacgaggac cgcttccact tcatcgactg gacgaagcgc gcgttccgca
     1681 acgtcgacgt gatcccgccg ggcaacggca tcatgcacca gatcaacctg gagcggatgt
     1741 cgccggtcgt gcacgcgcag gacggcgtcg ccttcccgga tacgctggtg ggtaccgaca
     1801 gccacacgcc gcacgtcgac gcgctgggcg tgatcgcggt gggcgtgggc ggcctcgagg
     1861 ccgagaacgt gatgctgggc cgcgcgtcgt ggatgcggct gccggagatg gtgggcgtcg
     1921 agctgagcgg caagctggcg gcgggcctga cggcgaccga cctcgtgctc gcgctcaccg
     1981 agttcctgcg caagtccaag gtcgtcggcg cctacctcga gttccgcggc cccggcgccg
     2041 cgacgctcac gctgggcgac cgcgcgacga tctccaacat ggcgcccgag tacggcgcga
     2101 cggcggcgat gttcgcgatc gacgaccaga cgctcgccta cctgcgcctg accggccgcg
     2161 cggacgagca ggtcgcgctg gtcgaggcct atgcgaaggc cgcgggcctg tgggccgccg
     2221 acctggcgca cgcgacctac gaccgcacgc tgtcgttcga cctgtccacg gtcgtgcgca
     2281 acatggccgg gccgtcgaac ccgcacgcgc gcgtcgcgac cgccgacctc gccgccaagg
     2341 gcatcgccgg cgcgtggacc gagacgccgg gcctcatgcc cgacggcgcc gtcatcatcg
     2401 cggccatcac gagctgcacc aacacgagca acccgcgcaa cgtgatcgcc gcgggcctgc
     2461 tcgcgcgcaa cgccaaccgc gcgggcctgg cgcgcaagcc ctgggtcaag agctcgctcg
     2521 cgccgggctc caaggccgtg acgctgtacc tcgaggacgc cggcctgctg cccgagctcg
     2581 agaaactggg cttcggcgtc gtcgcctatg cctgcacgtc gtgcaacggc atgtccggcg
     2641 cgctcgatcc gaagatccag caggagatcg tcgagcgcga cctctacgcg accgcggtgc
     2701 tctcgggcaa ccgcaacttc gacggccgca tccacccgta cgcgaagcag gccttcctcg
     2761 cctcgccgcc gctggtcgtc gcctacgcga tcgccggcac gatccgcttc gacatcgaga
     2821 aggacgtgct cggcaagacg gccgacggcc gggatatccg cctggccgac ctctggccga
     2881 ccgacgcgga ggtcgacgcc atcctcgccc gcagcgtgaa gcccgagtac ttccgccagg
     2941 tctacgagcc gatgttcgcg atccgtcccg cggacgccgc gagcgtgacg ccgctgtacg
     3001 actggcgccc gcagtcgacc tacatccgcc gtccgcccta ctgggaaggc gcgctggccg
     3061 gcgagcgcac gctgcgcggc atgcggccgc tggcgatcct gccggacaac atcacaaccg
     3121 accacctctc gccgtccaac gcgatcctgc cggacagcgc cgcgggcgag taccttgcgc
     3181 gcatgggcct gccggaggag gacttcaact cctacgccac ccaccgcggc gaccatctca
     3241 ccgcgcagcg cgcgaccttc gccaacccca cgctgaagaa cgagatggtg cgcaacgccg
     3301 acggcggcca gcgcgcgggc tcgctcgcgc gcgtcgagcc cgagggccgg gtgatgcgca
     3361 tgtgggaggc gatcgaggcc tacatggcgc gccgccagcc gctgatcatc gtcgccggcg
     3421 ccgactacgg ccagggctcc tcgcgcgact gggcggccaa gggcgtgcgc ctcgcgggcg
     3481 tcgaggcgat cgtggccgag ggcttcgagc gcatccaccg caccaacctg gtcggcatgg
     3541 gcgtgctgcc gctggagttc ctgcccggca ccgaccgccg cacgctgggc ctggacggca
     3601 ccgagaccta cgacgtcgtc ggcgcgcgca cgccgcgggc gaccctcacg ctggtggtgc
     3661 atcgccgcga cggcacgcgc gtggacgtgc cggtgacctg ccgcctcgac accgccgagg
     3721 aagtgtcgat ctacgaggcc ggcggcgtgc tccagcgctt cgcgcaggac ttcctcgcgt
     3781 cgacgaaaca ggccgcctga cgcggccgca atccatcatg agctcgatac cccagatccg
     3841 catccccgcc acgtacctgc gcggcggcac cagcaagggc gtgttcttcc gcctcgacga
     3901 cctgcccgag cgcgcgcgcg tgcccggccc cgcgcgcgac gcgctgctga tgcgcgtcat
     3961 cggcagcccc gacccgtacg gcaagcagat cgacggcatg ggcggcgcga cctccagcac
     4021 cagcaagacg gtcatcgtct cgccgagcac gcgcgcgggc cacgacgtcg actacctgtt
     4081 cggccaggtg gcgatcgaca agcgcttcgt cgactggagc ggcaactgcg gcaacctgtc
     4141 cgcggcggtc gggccgttcg cgatcgcgag cggcctgatc cccgcggagc gcgtgccgcg
     4201 cgacggcctc gccaccgtgc gcatctggca ggccaacatc ggcaagacca tcgtcgccca
     4261 cgtgccgatg agcggcggcg cggtgcagga gaccggcgac ttcgagctcg acggcgtgac
     4321 cttccccgcc gccgaggtgg cgctcgagtt cctcgacccc gccgacgagg gcgacggcga
     4381 aggcggcggc gcgatgttcc cgaccggcaa cgtcgtcgac cgcctcgagg tgcctggcgt
     4441 cggcaccttc gacgccacgc tgatcaacgc gggcatcccg acgatcttcc tgaacgccga
     4501 cgcgatcggc tacaccggcg cggagctgca ggacgcgatc aacggcgacc cgaaggcgct
     4561 cgcgatgttc gaggcgatcc gcgcgcacgg tgcggtgcgc atgggcctga tcgcgcacgt
     4621 cgacgaggcc gcgggccgcc agcacacgcc caaggtcgcc ttcgtcgcgc cgccgaagga
     4681 ctacgtcgcc tccagcggca aggcggtgaa ggccgccgac gtcgacctgc tggtgcgcgc
     4741 gatgtccatg ggcaagctgc accacgcgat gatgggcacg gccgcggtcg cgatcggcac
     4801 ggcggcggcg atcccgggca cgctggtcaa cctcgcggcc ggcggcggca cgcgcgacgc
     4861 ggtgcgcttc ggccatccgt cgggcacgct gcgcgtcggc gccgaggcga cgctggtcga
     4921 cggcgcctgg gtcgtgacca aggcgctcat gagccgcagc gcacgcgtcc tcatggaagg
     4981 ctgggtgcgc gtccccggcg atgcattttg agcccccacg gtcgctcgcg ctcgacgtac
     5041 gcttgaatca acgaggaatc cccatgtcca ctcgcaagac ccttcgcgcg ctcgccgagg
     5101 cccgccgcgg cgtgctcgtg ccgggcgcct tcaacgcgct gtcggcgcgc gtgatcgccg
     5161 acctcggctt cgaggcgatc tacgtcaccg gcgccggcgt caccaacatg tggttcggca
     5221 tgcccgacca gggcttcatg ggcctgcacg agatcgccga ccacacggcc cgcatccgcg
     5281 acgcggtgga gctgccgctg atcgtcgacg ccgacaccgg cttcggcaac gcgctcaacg
     5341 tgcgccacac cgtgcgcgtg ctcgagcgcg cgggcgcgga ctgcatccag ctcgaggacc
     5401 aggtcgcgcc caagcgctgc ggccatttca cgggcaagga agtgatctcc accgaggagg
     5461 ccgtcgccaa gatcaaggcc gcggtcgacg cgcgccacga tcccgacttc ctcgtgatgg
     5521 cccgcacgga cgccgccgcg acgcacggct tcgaggccgc ggtggagcgc gcgcagcgct
     5581 tcgccgaggc cggcgccgac atcctgttcg tcgaggccgt cacgaccgcc gacgagatcc
     5641 gcgcgctgcc ccggcgcctc ggtccgccgc agctgatgaa catggtcatc ggcggcagga
     5701 cgcccatctt cggcgccgag gagctcggca cgctcggcta cggactcgtg ctctacgcca
     5761 atgccgcgct gcagggcgcg gtcgccggca tgcagaaggt gctgaccgtt ctgcgcgaca
     5821 cgaagcgcgt cgacgaggat cccgcgctgg tcgcgccgtt caaggaacgc cagcgcctgg
     5881 tcggcaagcc cgagtgggac gcgctcgaaa agaagtacac ctgagcctga ccgatcgcga
     5941 tgcgaccccg ccttcgccgg cggggttcgc gttcccgggc ctcgtcagaa gctcacgccc
     6001 agccccaggt ccacccggct gaagtcggtg ttgacgttga agtgctggta gtcggccttc
     6061 agcaccaggt gcgagttgac gaggtagttc gcgcccagcg tggtcacgcg gtcgtgcggc
     6121 tgcggccagg gcttgccgtc ggaggcgagg ccggtgggcg tcgtcgagaa gcccggcgcg
     6181 atgcccgcat aggacgcgcc catgtcatag tgctcccagc gcacgaaggg gctgaacctg
     6241 gcgtcgccgt cctgccacag cgtgtacgcg gcctgcgcgt agtagccgag gaacgacgaa
     6301 ggcagcggat tggcgacgcc ggcgttggcg atgttgtagg ccgcggtgtg cgagatcgtg
     6361 cccttggcga agacggcctg gatgtcggcg gcgccgggcg tccagcgcgc gtgcgcctcc
     6421 cacagcgtcg tgcgcgcctg cggcaggccg gcgggcaccg cgggcatcgc ggcatcgccg
     6481 gtgaagaacg cgccgccgac cagcaggccc ggcacgccgc ggtagttcag cgccgcgaac
     6541 tgcgcgaggt gcgaggcgtc ggccagctgc agctcctggt gcgtggcctg gaagggggcg
     6601 acgtcgttgt cctcgagttc cagcgcggtg cggtactgcg gatcctcggg gctgttgttc
     6661 cagcccgaga ggttcagccc ggtcgtgagc ccggcgtccc agctcaggcc gaggtcgctc
     6721 gtgccgtgca gcgcgaggcc accctcgcgc caggtgctcg ggatgaccag cgtctcgacg
     6781 aagttgcgct gcacgccgta gaacgcggtc ggctcgtggt gctcgttgag caggccgaac
     6841 ggcatcagga acaggccgcc cttgccgctc acctgatcgg tgaactggtg gtcgacgtag
     6901 aactgctcga cctcgaactc gccccggtcg tcggcggacg cgacggcgtg ctccacctcg
     6961 aactccgagt tgaagctggt gcggtcgtcg aggcggtagc cgatgccgaa cacggcacgc
     7021 gcgaggtcgg cctgcgtgcg gctggcgtcg tgcaccggat gcgtgtagta cagctcgccg
     7081 tagccccaca gcgagaggtc gggcgacagt cccggcgcgg acacgggagc gggcgccgcg
     7141 acggccacgg gcgcaggcgc aggcgcggcc tgtcgctgcg ccgcctgcgt ctgcatctgc
     7201 tgcagcgcct gcttgacggc gtcgagctgc tgctgcaact gctggatctg ctgctcgagc
     7261 ttctgctgcg cgtcggtcgg gaggtccgcg gcgcgcgccg cggggacgag ggcggcacac
     7321 aacgcggccg catgcagggg gaggtgtcgc atcgggttca ggggttccgg ggatgggagg
     7381 acggggcggc cgccccgcgg gtcgcggcca cgcactgcgc gggcggggcc tgcgcgtcgg
     7441 gatgggcggg gtcgtagccg tcggacagcg tgcagaggaa ggcgatcacg tcgtcgatct
     7501 ccgcggggga tagcgccggc gcgtcgccga tgtggcggtc gtagggaatc tccagcgtgt
     7561 tgacgttgcc gtagtagccg aggtcgctgc ccgccacgcc ggcctgcacg accacctggc
     7621 ctccgtaggc cgccggcagg tcgtcgaact tggtcacgcg gccggcggcg tcggtcgggt
     7681 accactcctc gggattcgtg tcgcggcgca cgtagaagcc gatcgcctgc tgcagcgtcg
     7741 cgaagcggcc gttgtggaag tacggcgcgg tcaacgcgac gttgcgcagc gtcggcacct
     7801 tgaactggcc gcacaggccg ggcaccagca ggccgccgtt gtcgcggaac ggaccgcaga
     7861 tgccgaggtc gtagtacgcc tgcacgccgt cgctggaatc ggccggcgtg tacgcgggcg
     7921 cgccggcgcc ggcattggcc gggagggcgg cgttgcgcgg gacgccgagg ttgtcgaagc
     7981 tgaagtcggt gaacagcggc ggcgtgatgc cgtcgcccga ggtgctcggg tggcaggcgg
     8041 cgcagttgcc tttcgcgggg tcgttgaaca ggcccagccc gtcgagctcc tgcgccgtca
     8101 gctgcgcccg gccggcgcgc cagtagtcgt acttgctcgt gaacgggtgg aactcggggg
     8161 cctctgtttc gaacgccgcg agcgcctgcg ccatgcgccg cagggcgacc tcgggcgagg
     8221 cgagcgcatc gctgccgtac agcgcggtga actgcgcgag gtagggccgc gtcttcagct
     8281 tcgcgaccac ggcggcggcg tcggcgttgg ccatctcgaa gggcgtcgtg aagggcagcg
     8341 ccgcctggtc ggccagcgtg gcggccctgc cgtcgcggaa gaagccgccg ctggggctgc
     8401 catcggcggc gaacgagaac gccggcgcgt acgacgcgta cagcagcgac ggcgtgttgc
     8461 ggaagccggg ctggtccatg cccggcccgc ccagcggcac ggccagcccg tggtcgggcc
     8521 cgctggcgcc cggatcggcg gccatcgcga aggccttcgc atgacaggtc gcgcaggact
     8581 gcttgcccga ctccgacagc gccgtgtccg cgaagatcct ctggccgagt tgcgccgccg
     8641 ccgacatcgc cgccgggtcg gagggcgtgc cgcccggccc ggcggaatcg gacccgcccc
     8701 cgccgcacgc agcgagcgcc agcgtggcca gcagcgccgc cgcccgcccc cgcacacgac
     8761 ccgcggttcg cacgatgaaa ttcaagccgg cgactgactt cacgatggat attgacgata
     8821 ataggaataa ttctcgttcg tatcataacc tccgcactca tccacgatca acgttccggg
     8881 cactgcgcgg cgagcgatcc gttgcgtcgc cgccatgtct gggacaatcg ccgcttcccg
     8941 cggcgggccg gcgcccgtcc ggccgaaacg tctgcaagga aagcgctccc gatgaagtgg
     9001 tctccctccg gccttgcgcc gatcgcgctc gccgtgccgg cggccgtcgc gccccagctg
     9061 tgcgcagcga aggtgttcct gagcgtcgag caggcgcaga aggacatgtt cggcgcggcc
     9121 acgctcgcgg caacgccggt cgtgctgacc gcggcgcagc aggatcggct gaaggaggtg
     9181 tcgtccgtgc cgtcgccgtt ccagggcaac cgggtctggc gcgcatccga cggcggctgg
     9241 ttcgtcgtcg acgaggtcgt gggcaagcac gagctgatca cgtacgcggt cggcctgaac
     9301 gccgacggca cggtgcgccg cgtcgaggtg ctcgagtacc gcgagagcta cggcggcgag
     9361 gtcgcgggcg cgagctggct gaagcagttc gccggcaagt cggccgccgc gccggtcaag
     9421 gtgggccagg acatcgccaa catctccggg gcgacgctgt cgtccaggca cctcgcggac
     9481 ggcgtgcacc gcgtgctgga gctgtacgcg agcgtcctgc gcacgatggg atgagcgccg
     9541 caccgaccgg cctggcgcgc tgccgccccg cgctgggcac tctcgtccat gtcgcgctgc
     9601 gcgacgacct gccgcacgac gcgctgctcg cggcggtcga cgcggcattc gcggccatcg
     9661 aggcggtcga acgcgcgatg tccttccatc ggccggacag cgacctctcg cgcctgaacg
     9721 ccgcgcccgc cggggcgacg ctggcactgg acccggggct ggtcgaggtg ctgcgcttct
     9781 cggccgagct ccacgcgctg agcgacggcg tcttcgaccc gggggtcgcc gatcgcctgg
     9841 tcgcctggga cgtcctgccg cgccccgccg ccgcccaccc gccgctcgcc ggccgcgcga
     9901 cctccatccg cgacatcgag atcgtcgacg cccgacacgt gcgggtcctg cgtgccacct
     9961 gcctcgacct gggcggcgtc gccaaggggt tcgccgtgga ccgcacgatc gaggcgctgc
    10021 gcgcgcacgg gatcgcgcag gccgacgtca acgcgggcgg cgacctgcgc gtgctcggcg
    10081 agccgcgccc cgtgcacgt
//
LOCUS       DASVLN010000637         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_87322, whole genome shotgun sequence.
ACCESSION   DASVLN010000637 DASVLN010000000
VERSION     DASVLN010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87322"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1201)
                     /locus_tag="VF453_15200"
     CDS             complement(92..1201)
                     /locus_tag="VF453_15200"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7689060.1"
                     /translation="MKDSTRLLRLSALGVGVAAATLVLSACGKKHDATQAAARVDGTE
                     ITVHEINFRLQRERGLRSDQMDAESRKILDKLIDEQLFVAKAEKQGVDKQPEARQAID
                     AVRRDVLVHAYLDQTAQAVPAPTDDAVRRYYDGNAELFSNRRIYTLHEFLARVPADKQ
                     AALKALIDGGKPVADAVAWLKANGVEFREQETTRAAEQIPLGPLKQLAPLQEGQGLMA
                     TSGEQVHLTFVAAAKSEPVSFERAKPAISQFLIAEARRKNAAVNLEALRTSAKIEYAP
                     AYASLAASGPALATLHDAAASEVVPEAPGERVSLPTAPASGVTVTLPESPSSGVRVSL
                     PAATDQPGVRVSLPSTPASSVEVRLPPQTLPPSKK"
     gene            complement(1246..2568)
                     /locus_tag="VF453_15205"
     CDS             complement(1246..2568)
                     /locus_tag="VF453_15205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX7689061.1"
                     /translation="MKITVIGSGYVGLVTGACLAEMGNHVVCLDIDPCKIETLNSGGI
                     PIHEPGLEQVVQRNAAAGRLQFTTDTALAVAHGTLQFIAVGTPPDEDGSADLQYVLAA
                     ARNIGRLMTDYKLVIDKSTVPVGTADKVKAAVQAELDARGVKLEFSVASNPEFLKEGA
                     AVEDFMKPDRIVVGAEGERAISLLRAVYAPFQRNRDKLVVMDVRSAELTKYAANAMLA
                     TRISFMNELALLAESLGADIELVRQGIGSDPRIGYHFLYAGCGYGGSCFPKDVKALIR
                     TAAENGHGLKVLQAVEDANDAQKQVLVGKIVRRFGEDLAGRRFAVWGLAFKPNTDDMR
                     EASSRVLIGELLARGAAVTAYDPVAMTEARRVFGEEPRIAYANTPEQALEGADALAIV
                     TEWKEFRSPDFAHIAATLKTPVVFDGRNLYEPQLVREAGIDYQPIGRV"
BASE COUNT          363 a          935 c          980 g          428 t
ORIGIN      
        1 aggagcgcgg cgacggtcgt cgcgaggacg gcggcgacac ggacgacgcg gcgggcggcg
       61 ggcgatgaca ggcgggtcat ggaaactcct tctacttctt gctcggggga agcgtctgcg
      121 ggggcaggcg cacttcgacg ctcgaggcgg gcgtcgacgg caacgagacg cgcacgcccg
      181 gctgatccgt ggcggcgggc agggagacgc gcacgcccga cgacgggctc tcgggcagcg
      241 tgacggtcac gccggaagcg ggcgccgtcg gcaggctgac gcgctcgccc ggcgcctccg
      301 gcaccacctc ggacgcggcc gcgtcgtgca gcgtcgcgag cgcggggccg gacgcggcca
      361 gcgaggcgta ggccggcgca tattcgatct tggccgacgt gcgcagcgcc tcgaggttga
      421 ccgcggcgtt cttgcggcgg gcttccgcga tcaggaattg cgagatcgcc ggcttggcgc
      481 gctcgaagct cacgggttcg gacttggccg cggccacgaa ggtgaggtgg acctgctcgc
      541 cggaggtcgc catcaggccc tggccctcct gcagcggcgc gagctgcttg agcggcccga
      601 gcgggatctg ttcggcggcg cgcgtggtct cctgctcgcg gaactccacc ccgttggcct
      661 tcagccaggc gacagcatcg gccaccggct tgcccccgtc gatcagggcc ttcagcgcgg
      721 cctgcttgtc ggccggcacg cgcgcgagga actcgtgcag cgtgtagatg cgccggttgg
      781 agaacagctc cgcattgccg tcgtagtagc ggcgcaccgc gtcgtcggtc ggcgcgggca
      841 ccgcctgcgc cgtctggtcg aggtaggcgt gcacgagcac gtcgcggcgc acggcgtcga
      901 tcgcctgccg cgcctcgggc tgcttgtcga cgccctgctt ctcggccttc gcgacgaaca
      961 gctgctcgtc gatcagcttg tcgaggatct tgcggctctc cgcatccatc tggtcggagc
     1021 gcaggccgcg ctcgcgctgc aggcggaagt tgatctcgtg gacggtgatc tcggtgccgt
     1081 ccacgcgcgc ggcggcctgc gtcgcgtcat gcttcttgcc gcacgcagac agcaccagcg
     1141 tcgccgcggc gacgcccacg cccagggccg acaggcgcag gagccgggtc gaatccttca
     1201 tgtgacgcat tccctccgac ccgaatcggg ctccgttcct gattttcaca cgcgtccgat
     1261 cggctggtag tcgatgccgg cctcgcgcac gagttgcggc tcgtacaggt tgcggccgtc
     1321 gaagacgacg ggcgtcttca gcgtcgcggc gatgtgggcg aagtccgggc tgcggaattc
     1381 cttccactcg gtgacgatcg ccagcgcgtc ggcgccctcc agcgcctgct ccggcgtgtt
     1441 cgcgtaggcg atgcgcggct cctcgccgaa gacgcgccgc gcctcggtca tcgcgaccgg
     1501 gtcgtaggcc gtgaccgcgg cgccgcgggc gagcagctcg ccgatcagca cccgcgagct
     1561 cgcctcgcgc atgtcgtcgg tgttgggctt gaacgcgagg ccccagaccg cgaagcgcct
     1621 gcccgccagg tcctcgccga agcggcgcac gatcttgccg accagcacct gcttctgcgc
     1681 gtcgttcgcg tcctcgaccg cctgcagcac cttcaggccg tgcccgttct ccgcggcggt
     1741 gcggatcagc gccttgacgt ccttcgggaa gcaggagccg ccgtagccgc agccggcgta
     1801 caggaagtgg tagccgatgc gcggatccga gccgatgccc tggcgcacga gctcgatgtc
     1861 cgcgcccagc gactccgcca gcagcgcgag ctcgttcatg aaggagatgc gcgtcgcgag
     1921 catggcgttg gcggcgtact tcgtgagctc ggcggagcgc acgtccatca cgaccagctt
     1981 gtcgcggttg cgctggaacg gcgcgtagac cgcccgcagc agcgagatcg cccgctcgcc
     2041 ctcggcgccg acgacgatgc gatcgggctt catgaagtcc tcgaccgccg cgccttcctt
     2101 caggaactcg gggttggacg cgacggagaa ctcgagcttc acgccgcggg cgtccagctc
     2161 cgcctgcacg gccgccttga ccttgtcggc ggtgccgacg ggcacggtgc tcttgtcgat
     2221 gaccagcttg tagtcggtca tcaggcgccc gatgttgcgg gcggccgcca gaacatactg
     2281 caaatccgcc gacccgtcct cgtccggcgg cgtgccgacc gcgatgaact gcagcgtccc
     2341 gtgcgcgacg gccagcgccg tgtccgtcgt gaactgcagg cggcccgccg cggcgttgcg
     2401 ctggacgacc tgctccaggc cgggctcatg gatcgggatg ccgccgctgt tgagcgtctc
     2461 gatcttgcag ggatcgatgt cgaggcagac gacgtggttg cccatctccg cgaggcaagc
     2521 gccggtgacg aggccgacgt agccggagcc gatgactgtg attttcatgg agggttcggg
     2581 aagtcaggaa gtcgtctggt cgagctgggc gaaggcgcga actcgggcag ctttcgtgcc
     2641 cgggctcact gcgaggccgc gctggcggcc tcgacggcga gcggggccgc cgccggctcc
     2701 gacgca
//
LOCUS       DASVLN010000638         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_142929, whole genome shotgun sequence.
ACCESSION   DASVLN010000638 DASVLN010000000
VERSION     DASVLN010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142929"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VF453_15210"
     CDS             complement(<1..780)
                     /locus_tag="VF453_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7689062.1"
                     /translation="MTSPLHSLLARLKDQISTIIVGKPSQVDDAVACLLAGGHLLIED
                     VPGVGKSTLAHVLAVSLGLKFSRVQFTADLLPSDLIGVSVYERAKESFAFHPGPVFAQ
                     VLLADEINRAGPKTQSALLEAMEEHQVSVDGQTRPLPAPFFVIATQNPSDQLGTYPLP
                     ESQLDRFLMCITIGYPDARSERELLAGQDRREAVARLQPVMTPAQLVEAMAEVRRIHV
                     SDPLLDYLQALIAATRSGHWFADGLSPRASIGVLRLARARAL"
     gene            871..1809
                     /locus_tag="VF453_15215"
     CDS             871..1809
                     /locus_tag="VF453_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX7689063.1"
                     /translation="MTTAYFSHADCRRHEMGPGHPECPARLDAIEDHFLATGLADIVA
                     RHDDAPLADFADLARAHASGYLAGIDDTLARVAREGGYVAVDPDTAACPHTRQAALRA
                     AGAAVAATDLVLDGRADNAFCAVRPPGHHATRSEAMGFCFYNNVAIAARHALDVRGLA
                     RVAIVDFDVHHGNGTEDIVAGDERVLMVSIFQDQLYPFSGNEPLGDNMLNVPCPAYTR
                     GAQVREVVERLWLPRLDAFRPQMIFVSAGFDAHRDDELGRMGLVEADYAWITERIVEV
                     AARHAQGRIVSCLEGGYNLDALARSAAAHVRALAGV"
     gene            1916..>3258
                     /locus_tag="VF453_15220"
     CDS             1916..>3258
                     /locus_tag="VF453_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7689064.1"
                     /translation="MENWFSPELLALIQGLQKPGAYVALMLVLAAVPIAWGVPRLLRG
                     PTGKPHSIVFGSRIVDGVLFPALWLGLVWLARTLCMRAHLPLAVFRLAVPILLSLLLI
                     RLTVRVMRVAFPKSGVVRAVEKTVSWLAWIGVVLWITGVLPSFLDALDDVTWKMGASK
                     VSLATVLTGGLAAGIALLVTLWISSAVESWLLKGVAGQQLSWRKAAANAVRTVLVLVG
                     LLVALSAVGIDLTALSVLGGAVGVGLGFGLQKLASNYVSGFVILAERALRIGDTVKVD
                     GFEGRITDITTRYTVLRALNGRESIVPNELLITQRVESSTLADSRMALSTVVQVGYET
                     DLDVLMPRILEVVQPIRRVIADPSPSVVLSAFAADGLELTVSFWIADPENGTGGVKSD
                     VNLALLRLFNEQGVEIPYPQRVVRTLAVEPGREAEGVVPGEGVARATRAVVGDPRG"
BASE COUNT          430 a         1176 c         1182 g          470 t
ORIGIN      
        1 cagggcgcgc gcccgcgcga ggcgcagcac gccgatgctg gcgcgcggcg acagcccgtc
       61 ggcgaaccag tgccccgagc gcgtcgccgc gatcagcgcc tgcaggtagt cgagcagcgg
      121 atcggagacg tggatgcgcc gcacctcggc catcgcctcg accagctgcg ccggcgtcat
      181 caccggctgc agccgcgcga ccgcctcgcg ccgatcctgg ccggcgagca gctcgcgttc
      241 ggagcgcgcg tccgggtagc cgatcgtgat gcacatcagg aagcgatcca gctgcgactc
      301 cggcagcggg taggttccga gctggtcgct cgggttctgc gtcgcgatga cgaagaaggg
      361 cgcgggcagc gggcgggtct ggccgtcgac gctgacctgg tgttcctcca tcgcctcgag
      421 cagcgcgctc tgcgtcttcg ggccggcgcg gttgatctcg tcggccagca gcacctgcgc
      481 gaacaccggc cccggatgga aggcgaacga ctccttggcg cgctcgtaga cgctgacgcc
      541 gatcaggtcg ctcggcagca ggtcggcggt gaattgcacg cgcgagaact tcaggcccag
      601 cgagacggcc agtacgtggg cgagcgtgct cttgccgacg ccgggcacgt cctcgatgag
      661 caggtggccg ccggccagca ggcaggcgac ggcgtcgtcc acctgggacg gcttgccgac
      721 gatgatcgtg ctaatctggt ccttgagccg ggccaggagc gaatgcagcg gtgaggtcat
      781 ggcggcaacg atagcccaac caggagacgg agcgcccggg cgggcgcggc ggacgttcat
      841 gagcagcacg acattcccgc ggacgacgcg atgaccaccg cctatttcag ccacgccgac
      901 tgccgccgcc acgagatggg acccgggcat cccgagtgcc cggcccggct cgacgcgatc
      961 gaggaccact tcctcgcgac gggactggcc gacatcgtcg cccgccacga cgacgcgccg
     1021 ctcgccgact tcgccgacct cgcccgcgcc cacgcgagcg gctacctcgc cggcatcgac
     1081 gacacgctgg cgcgggtcgc gcgcgaaggc ggctacgtcg cggtcgaccc cgacaccgcc
     1141 gcatgcccgc acacccgcca ggccgcgctg cgcgccgccg gcgcggcggt cgcggcgacg
     1201 gacctcgtcc tcgacggccg cgccgacaac gccttctgcg ccgtgcgccc gccgggccac
     1261 cacgccacgc gcagcgaggc gatgggcttc tgcttctaca acaacgtcgc gatcgccgcc
     1321 cgccacgcgc tggacgtgcg cggcctcgcg cgcgtggcca tcgtcgactt cgacgtccac
     1381 cacggcaacg gcaccgagga catcgtcgcc ggcgacgagc gggtcctgat ggtgagcatc
     1441 ttccaggacc agctctaccc gttcagcggc aacgagccgc tcggcgacaa catgctcaac
     1501 gtgccgtgcc ccgcctacac ccgcggagcg caggtgcgcg aggtcgtcga gcggctgtgg
     1561 ctgccgcgcc tggatgcgtt ccgcccgcag atgatcttcg tgtccgccgg cttcgacgcg
     1621 caccgcgacg acgagctcgg gcgcatgggc ctcgtcgagg ccgactacgc gtggatcacc
     1681 gagcgcatcg tcgaggtcgc cgcccgccac gcgcaggggc gcatcgtcag ctgcctggag
     1741 ggtggctaca acctcgacgc gctggcgcgc agcgcggcgg cgcacgtgcg cgcgctggcc
     1801 ggggtctgag aatcacacct tctgagcgcg ccgaggcggt gcccgcgccc gtcgcgcagc
     1861 gccgcgcggc cggcgcgccg cccgcgcccg ggcgcttcgg ccagaatcac gccccatgga
     1921 aaactggttc tctcccgagc tgctcgcgct gatccaggga ctgcagaagc ccggcgccta
     1981 cgtcgcgctg atgctggtcc tcgcggccgt gcccatcgcc tggggcgtgc cgcggctgct
     2041 gcggggcccg acgggcaagc cgcactcgat cgtgttcggc agccgcatcg tcgacggcgt
     2101 gctgttcccg gcgctgtggc tggggctggt gtggctcgcg cgcacgctgt gcatgcgggc
     2161 gcacctgccg ctggcggtgt tccggctcgc ggtgccgatc ctgctgtcgc tgctcctgat
     2221 ccggctgacg gtgcgcgtga tgcgcgtggc gttcccgaag tccggagtcg tgcgggcggt
     2281 cgagaagacc gtctcctggc tcgcgtggat cggcgtcgtg ctgtggatca cgggcgtgct
     2341 gccgtccttc ctcgatgccc tggacgacgt cacctggaag atgggcgcga gcaaggtctc
     2401 gctcgcgacg gtgctcacgg gcggcctggc cgcgggcatc gcgctgctgg tgaccctgtg
     2461 gatctcgtcg gcggtcgaga gctggctgct caagggcgtc gccggccagc agctgtcgtg
     2521 gcgcaaggcc gcggccaacg ccgtgcgcac ggtgctggtg ctggtcggcc tgctggtcgc
     2581 gctgtcggcg gtggggatcg acctcaccgc gttgtcggtg ctgggcggcg cggtcggcgt
     2641 ggggctgggc ttcggcctgc agaagctcgc gtcgaactac gtcagcggct tcgtcatcct
     2701 cgccgagcgc gcgctgcgca tcggcgacac ggtgaaggtc gacggcttcg aaggccgcat
     2761 caccgacatc acgacgcgct acaccgtcct gcgcgcgctc aacggccgcg agtccatcgt
     2821 gcccaacgag ctgctgatca cccagcgcgt cgagagctcg acgctcgcgg actcccgcat
     2881 ggcgttgtcg accgtcgtgc aggtcggcta cgagaccgac ctcgacgtcc tgatgccgcg
     2941 catcctcgag gtggtgcagc cgatcaggcg cgtgatcgcc gacccctcgc cctccgtggt
     3001 gctgagcgcc ttcgcggccg acggcctgga gctcaccgtc tcgttctgga tcgccgatcc
     3061 cgagaacggc acgggcggcg tcaagtccga cgtcaacctg gcgctgctgc ggctgttcaa
     3121 cgagcagggc gtcgagatcc cgtacccgca gcgcgtcgtg cgcacgctgg cggtcgagcc
     3181 cggccgcgag gcggagggcg tcgtgccggg cgagggcgtg gcgcgggcga cccgggcggt
     3241 cgtgggcgac ccgcgcgg
//
LOCUS       DASVLN010000639         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_518679, whole genome shotgun sequence.
ACCESSION   DASVLN010000639 DASVLN010000000
VERSION     DASVLN010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518679"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VF453_15225"
     CDS             complement(<1..382)
                     /locus_tag="VF453_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEX7689065.1"
                     /translation="MSAAPSSTHVEVFLKPGEYVVAGPGHRFRTVLGSCISMTLWSPH
                     RRVGAMSHFLLPTRGRGRVDATRGLALRALDGRYGDEALQLMLHELDRLDVRPAQCEA
                     KIFGGGDMFPGRRRAGGPLAVGRRN"
     gene            complement(379..1182)
                     /locus_tag="VF453_15230"
     CDS             complement(379..1182)
                     /locus_tag="VF453_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX7689066.1"
                     /translation="MNTLTDTEFTRFQRFIYDAAGITLTPAKKALVCGRLSKRVAAHE
                     LASFGEYFELLSDGRHAAEVQTAVDLLTTNETYFFREPRHFEMLRELAARHGGRGPAF
                     RVWSAASSSGEECYSIAMVLADVLGQAPWEVVGTDISTRVLQRARTGHYPLERTRNIP
                     PAYLKRYCLRGTGEQAGTLLVERALRQRVKFAHVNLNADLPQLGGFDVVFLRNVMIYF
                     NGDTKRRVVARVLSQLEPGGHLFIGHSESLNDISTAVEQLAPSIYRKPG"
     gene            complement(1179..2435)
                     /locus_tag="VF453_15235"
     CDS             complement(1179..2435)
                     /locus_tag="VF453_15235"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HEX7689067.1"
                     /translation="MTSSASTAPGIGSLLVVDDSPVQRGHAAALCRQLGVGTVHEAAS
                     GAEALGLLERLASSPVLAIVDLEMPVMDGIEFIQRLAQGPHALPLVVVSGREQALIET
                     VETMARNLGLTVLAALRKPLRQEALADALAAVGAAAIGIREGERAPEPQIDAAALARA
                     IDGDRIDVHVQPKADMLTGLVRGVEVLARWTDDEGTAVRPDRFVALAEREGLILGLTR
                     RVMARAFAQAAYWNARGLRLSMAVNLSPRLLEAPGLVEEISALVARHGLRPDQVVFEI
                     TESSVVEALGAALGVLARLRLQGFGLSIDDYGTGFSSMQQLARVPFTELKVDRSFVHD
                     ACRRENLRVILQSAVDMARQLGLVTVAEGIETMEDWRLVQQCGCHIGQGWLIARAMPP
                     ADLLPWIKNHQRSLPGLRPQRADATR"
BASE COUNT          356 a          890 c          948 g          332 t
ORIGIN      
        1 cgttgcggcg gccgacggcc agcggcccgc cggcgcgccg gcggcccggg aacatgtcgc
       61 ccccgccgaa gatcttggcc tcgcactgcg cgggccgcac gtccaggcga tccagttcgt
      121 gcagcatcag ctgcagcgcc tcgtcgccgt agcggccgtc gagggcgcgc agcgccaggc
      181 cgcgcgtcgc gtcgacgcgc ccgcggccgc gcgtgggcag caggaagtgc gacatcgcgc
      241 cgacccgccg gtgcgggctc cacagcgtca tcgagatgca ggagccgagc accgtgcgaa
      301 agcggtggcc ggggcccgcg acgacgtatt cgccgggctt caggaagacc tcgacgtgcg
      361 tcgaggatgg cgccgcgctc atcccggctt gcggtagatc gagggggcga gctgctcgac
      421 cgccgtggag atgtcgttca ggctctcgga atggccgatg aacaggtgcc cgcccggctc
      481 cagctgggac agcacgcgcg cgaccacgcg gcgcttggtg tcgccgttga agtagatcat
      541 cacgttgcgc aggaagacga cgtcgaagcc gcccagctgc ggcaggtcgg cgttgaggtt
      601 gacgtgcgcg aacttcacgc gctggcgcag cgcgcgctcc accagcagcg tgccggcctg
      661 ctcgccggtg ccgcgcaggc aataccgctt gaggtatgcg ggcgggatgt tgcgcgtgcg
      721 ctcgagcgga tagtgcccgg tgcgcgcgcg ctgcagcacg cgcgtgctga tgtcggtgcc
      781 gacgacctcc cacggcgcct ggcccagcac gtcggccagc accatcgcga tgctgtagca
      841 ctcctcgccg ctggagctcg cggcgctcca gacgcggaac gcgggaccgc ggccgccgtg
      901 gcgcgcggcg agctcgcgca gcatctcgaa gtggcgcggc tcgcggaaga agtaggtctc
      961 gttggtcgtc agcaggtcca ccgcggtctg cacctcggcg gcatggcggc cgtcggacag
     1021 cagctcgaag tactcgccga agctcgcgag ctcgtgcgcg gcgacgcgct tggacagccg
     1081 cccgcagacc agcgccttct tggccggcgt cagcgtgatg ccggcggcgt cgtagatgaa
     1141 gcgctggaag cgcgtgaatt cggtgtcggt gagggtgttc atcgcgtcgc gtcggcgcgt
     1201 tgggggcgca ggccgggcag gctgcgctgg tggtttttga tccagggcag caggtcggcg
     1261 gggggcatgg cccgcgcgat cagccagccc tggccgatat ggcagccgca ctgctgcacg
     1321 agccgccagt cctccatcgt ctcgatgcct tcggccacgg tgacgagccc gagctggcgg
     1381 gccatgtcca ccgccgactg aaggatcacc cgcaggttct cgcggcggca ggcgtcgtgg
     1441 acgaaggagc ggtcgacttt caactcggtg aacggcacgc gcgcgagctg ctgcatcgag
     1501 gagaagccgg tgccgtagtc gtcgatggac aggccgaagc cctgcaggcg cagccgcgcg
     1561 agcacgccca gcgccgcgcc gagggcctcg acgaccgagc tctcggtgat ctcgaagacg
     1621 acctggtcgg gccgcaggcc atggcgcgcg acgagcgcgc tgatctcctc cacgagccct
     1681 ggggcctcca gcaggcgcgg cgagaggttg accgccatcg acagccgcag gccgcgcgca
     1741 ttccagtagg cggcctgcgc gaaggcgcgc gccatcacgc ggcgcgtgag gccgaggatg
     1801 agcccctcgc gctcggccag cgcgacgaag cggtcgggcc gcacggccgt gccttcgtcg
     1861 tccgtccagc gcgcgagcac ctcgacgccg cgcacgagcc cggtgagcat gtcggccttg
     1921 ggctgcacgt gcacgtcgat gcggtcgccg tcgatcgcgc gcgccagcgc ggcggcgtcg
     1981 atctgcggct cgggcgcccg ctcgccttcg cggatcccga tcgcggcggc gccgacggcc
     2041 gccagcgcgt cggccagcgc ctcttgccgc agcggcttgc gcaacgcggc gagcaccgtc
     2101 agcccgaggt tgcgcgccat cgtctcgacc gtctcgatca gcgcctgctc gcgaccggac
     2161 accacgacca gcggcagcgc atgcggtccc tgcgccaggc gctggatgaa ctcgatgccg
     2221 tccatcaccg gcatctcgag atccacgatc gcgaggacgg gcgacgaggc caggcgctcg
     2281 agcagcccga gcgcctcggc acccgacgct gcctcgtgga cggtgcccac gcccagctgg
     2341 cggcacagcg ccgccgcgtg gccgcgttgc accgggctgt cgtcgacgac cagcagcgag
     2401 ccgatgcccg gagcggtgga ggcggacgac gtcatgtggg cgagggcgcg ggccggcgag
     2461 gcggtcagaa gcgggcgaag tggctctcgt cgagcgcggg ctcggcgacg aaggccggcg
     2521 cgccgc
//
LOCUS       DASVLN010000640         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_194345, whole genome shotgun sequence.
ACCESSION   DASVLN010000640 DASVLN010000000
VERSION     DASVLN010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194345"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..889)
                     /gene="ccsA"
                     /locus_tag="VF453_15240"
     CDS             complement(95..889)
                     /gene="ccsA"
                     /locus_tag="VF453_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX7689068.1"
                     /translation="MILSFDPATALAWAAVAAYVLAAAARPPAAGFVQAAALMLHGGS
                     LVVRFALEARAGHGIRFGFAPVLSLTAWLVLTVHYMESRLLPIAGPRRIAPVIGAVAV
                     VLALAFPGEFVVVAYSAYAPIHWALGIAAYALFGVAVVHAAMLDAAERRMRRKQPTDA
                     HGVPLLSLERLTFNFVEAGFVVLTLAIVLGLVQTDEWHWDHKTVLSLASWATFAALIV
                     VRRTRGLRGRQATRWVYAGAVLMLLAYVGSRFVFEVVLQRLPAGGA"
     gene            972..2336
                     /gene="ffh"
                     /locus_tag="VF453_15245"
     CDS             972..2336
                     /gene="ffh"
                     /locus_tag="VF453_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7689069.1"
                     /translation="MASTLTDRLSRLVKTMRGQARITESNVQDMLREVRMALLEADVA
                     LPVVRDFIARVKDKALGQEVVSSLTPGQVLVGIVQKELAATMGEGIADIDLATQPPAV
                     ILMAGLQGAGKTTTTAKLARHLIQKRKKKVLTVSGDVYRPAAIEQLKTVTSQAGAEWF
                     PSTPDQKPRDIALAALDYARKHYFDVLLVDTAGRLAIDEALMAEIRELHATLKPVETL
                     FVVDAMQGQDAINTAKAFKEALPLTGIVLTKLDGDSRGGAALSVRQVTGAPIKFAGTS
                     EKIDGLEVFDAERHAGRVLGMGDIVALVEEVTKGVDVEAAKKLADKVRSGSDFDLNDF
                     LGQLQQMKKMGGLAGLMDKLPTQMTAKAGAADMDKAERDMRRMEGIINSMTPQERAKP
                     ELLKAKRKFRVAKGAGVQVQDVNRLLNQYEQMRDMMKKMKSGGLMKMMKRLGGMKGFP
                     GMPR"
     gene            2428..2967
                     /locus_tag="VF453_15250"
     CDS             2428..2967
                     /locus_tag="VF453_15250"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7689070.1"
                     /translation="MKLRLPFLLAAVVATLAGAAHAALKPGDVAPDFTTRAALGGKAM
                     DFKLADALKKGPVVLYFFPKSFTQGCTAEAHLFAEATDRFAALGATVVGISHDDIATQ
                     VKFSQEACRNKFAVGADPDSRIIKAYDAKLAMLPMSDRISYVIAPDGKVIYEYQSLDP
                     QNHVANTMKAVQDWKAAHR"
BASE COUNT          533 a         1110 c         1060 g          361 t
ORIGIN      
        1 ctgcgcgcgc acggcggccg gcgcgcgcgg gcggcggaac ttcgaccaga acaggacgac
       61 gaccgcgagc cagatcagca gccggatcag cacctcacgc ccctccggcc ggcagccgct
      121 gcagcacgac ctcgaagacg aagcgcgagc cgacgtaggc cagcagcatc agcacggcgc
      181 cggcgtagac ccagcgcgtg gcctggcgac cgcgcaggcc gcgcgtgcgg cgcacgacga
      241 tgagcgccgc gaaggtcgcc cagctggcga gcgacagcac ggtcttgtgg tcccagtgcc
      301 actcgtcggt ctgcacgagc ccgagcacga tggccagcgt cagcacgacg aagcccgcct
      361 cgacgaagtt gaaggtcagg cgctcgagcg acagcagcgg cacgccgtgc gcgtcggtcg
      421 gctgcttgcg gcgcatgcgg cgttcggcgg cgtccagcat cgcggcgtgc acgaccgcga
      481 cgccgaacag cgcgtaggcc gcgatgccca gcgcccagtg gatcggcgcg taggcggaat
      541 aggcgacgac cacgaactcg cccgggaacg cgagcgccag caccaccgcg accgccccga
      601 tcaccggcgc gatgcgacgc gggccggcga tcggcagcag gcggctctcc atgtagtgca
      661 cggtcagcac gagccaggcg gtcagcgaca acaccggcgc gaagccgaag cggatcccgt
      721 ggccggcgcg ggcctcgagc gcgaaacgca cgaccagcga ccccccgtgc agcatcaacg
      781 ccgccgcctg gacgaagccg gccgccggcg gtcgcgcggc ggccgccagc acgtaggccg
      841 ccaccgcggc ccacgcgagc gcggtggcgg ggtcgaacga tagaatcatc cgcgcagttt
      901 accggcgagc ggtcgacctg ccggcacggc ccggcacgcg tcgccccgac gccctcctct
      961 ttcgcccacc catggcctcc accctcaccg accgcctgtc gcgcctcgtc aagacgatgc
     1021 gcggccaggc ccgcatcacc gaatccaatg tccaggacat gctgcgcgag gtgcgcatgg
     1081 ccctgctgga ggccgacgtc gcgctgcccg tcgtgcgcga cttcatcgcc cgcgtgaagg
     1141 acaaggcgct cggccaggag gtcgtctcct cgctgacgcc gggccaggtg ctggtgggca
     1201 tcgtgcagaa ggagctcgcg gcgacgatgg gcgagggcat cgccgacatc gacctcgcga
     1261 cgcagccgcc cgccgtcatc ctgatggccg gcctgcaggg cgcgggcaag acgacgacga
     1321 ccgccaagct cgcgcgccac ctgatccaga agcgcaagaa gaaggtgctg acggtctcgg
     1381 gcgacgtcta ccgcccggcc gccatcgagc agctcaagac cgtcacgagc caggccggcg
     1441 ccgaatggtt cccgtccacg cccgaccaga agccgcgcga catcgcgctc gccgccctcg
     1501 actacgcgcg caagcactac ttcgacgtgc tgctggtcga caccgccggc cggctggcga
     1561 tcgacgaggc gctcatggcc gagatccgcg agctccacgc gacgctcaag ccggtcgaga
     1621 ccctgttcgt cgtcgatgcg atgcagggcc aggacgcgat caacaccgcc aaggccttca
     1681 aggaggccct gccgctcacc ggcatcgtgc tgaccaagct ggacggcgat tcgcgcggcg
     1741 gcgcggcgct gtcggtgcgc caggtcaccg gcgcgccgat caagttcgcc ggcacgtcgg
     1801 agaagatcga cggcctggag gtcttcgacg ccgagcgcca cgccggccga gtgctcggca
     1861 tgggcgacat cgtcgcgctc gtcgaggagg tcaccaaggg cgtcgacgtc gaggccgcga
     1921 agaagctcgc cgacaaggtg cgctcgggct cggacttcga cctcaacgac ttcctcggcc
     1981 agctgcagca gatgaagaag atgggcggcc tcgcgggcct gatggacaag ctgccgaccc
     2041 agatgaccgc caaggccggc gccgccgaca tggacaaggc cgagcgcgac atgcgccgca
     2101 tggagggcat catcaactcg atgacgccgc aggagcgcgc caagcccgag ctgctcaagg
     2161 ccaagcgcaa gttccgcgtc gccaagggcg ccggcgtgca ggtgcaggac gtcaaccgcc
     2221 tgctcaacca gtacgagcag atgcgcgaca tgatgaagaa gatgaagagc ggcggcctca
     2281 tgaagatgat gaagcgcctg ggcggcatga agggcttccc ggggatgccg cgctgaggac
     2341 ggcgcccccg gtcgccgggg ctgccggcac atgaaccacc tgccgcctgc gcggaacgcg
     2401 ccgaatcacc gtcgttggag aatcgccatg aagttgcgcc ttcccttcct gctcgccgcc
     2461 gtcgtggcga cgctcgccgg cgcggcacac gccgccctca agcccggcga cgtcgcgccc
     2521 gacttcacga cccgcgccgc gctcggcggc aaggcgatgg acttcaagct cgccgacgcg
     2581 ctgaagaagg gtccggtggt gctgtacttc ttcccgaagt cgttcaccca gggctgcacg
     2641 gccgaggcgc acctgttcgc cgaggcgacc gacaggttcg ccgcgctcgg cgcgacggtc
     2701 gtcggcatct cgcacgacga catcgcgacg caggtgaagt tctcgcagga ggcgtgccgc
     2761 aacaagttcg cggtcggcgc cgacccggat tcccggatca tcaaggccta cgacgccaag
     2821 ctcgcgatgc tgccgatgtc ggaccgcatc tcctacgtga tcgcgcccga cggcaaggtg
     2881 atctacgagt accagagcct cgacccgcag aaccacgtcg ccaacacgat gaaggccgtg
     2941 caggactgga aggccgcgca caggtgacgc gccgctgacg cgctcgtgac gcggcgggcg
     3001 tcgccgcggt cgcgggccgg tcaggcgcgg ccggtctcgc cgggcgcggc gtcgacgctc
     3061 gcgt
//
LOCUS       DASVLN010000641         3956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_194431, whole genome shotgun sequence.
ACCESSION   DASVLN010000641 DASVLN010000000
VERSION     DASVLN010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194431"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VF453_15255"
     CDS             <1..394
                     /locus_tag="VF453_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HEX7689071.1"
                     /translation="LTRAQLEAVLGGPLRAPGDDAPRASGTLEAHYAPRAKLRLMPAA
                     QLRTALQVLPKSTVNPVAVYSRTVAPGPSVRASRSMPDEPIAAAHELFAALRELDACG
                     ASLIWVETPPDGPAWEGVRDRLQRAAAA"
     gene            471..1460
                     /locus_tag="VF453_15260"
     CDS             471..1460
                     /locus_tag="VF453_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7689072.1"
                     /translation="MLFKNHLGRFATRAAAGLAAALALASCGGGTYQEHAFVPARILT
                     FGDESNSLIGTQGLKYSINGLNAQTHQIDCTVSPLWTQVLANSYAISYQRCNTQEVAS
                     PQGYDFSTVNATVADTANQVQAFLAGDSFNSDDLVTIWVGVHDILDDYATNGATAASG
                     DFSSLLNDAHGFGVSLAQVVNQVANSGAKVVLLTLPDMGESPFAYNENLRGDFNRADL
                     LSQMTQKFNDGLRANIVNDGSKIGLVLVDDFVRNAVRSPGSYGLITNPFAEAGCKASV
                     ALPDCTDDDTVTDAGKNSTLPSIFLWADATHLAPTAHAQIGSEAVSRAHTNPF"
     gene            1729..2859
                     /locus_tag="VF453_15265"
     CDS             1729..2859
                     /locus_tag="VF453_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7689073.1"
                     /translation="MKTFHVLTTALAAAAFLAGCGGNDAGDQSPRVAFKTMVNFGDSL
                     SDVGTYDVGAVALQGGGHYSINGKGATGLAYINWTEFLAATLQLPQPCAAETGLDTIQ
                     AVIGVPAVPATFHDTGATPCYDYAQGGARVTADVGPGNAALFNAQDQSTYSDAIGQLT
                     VPVKTQIANYTLHGGTFDGDDKLFTVFAGGNDLFMQVGIIQATPVAQQAAAAQAAGVA
                     MATAGAEEAAYVNQMIAAGAKHVVVVLLPDVSLTPYAISAGATGQALVLQLTQAFNAQ
                     LTKGLQASDSLLVVDAFSASQDQAAHMSQYGLINVTAPACTSSSSLVCTANTLVSTPD
                     DLAADPTKANGYQYADSVHPTPYGYKLLAQLVGEQMAIKGWL"
     gene            2896..3570
                     /locus_tag="VF453_15270"
     CDS             2896..3570
                     /locus_tag="VF453_15270"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX7689074.1"
                     /translation="MKKHSVASILVAAAALAAAGGAQAQQAGTFSVKVGWNKIMPKVK
                     SDDLSEPSLPGSKIDIHSASAAIITASYMFTDAIGIEALGGLPYKHNIYAAGAVEGVG
                     KIGSIHQISPTLLLEYRLLAADAPFRPYVGAGPTFAKFYGTKGSAQLTAVTNPGGSPT
                     TIGSDTEWGGTLAAGGRYRIDSHWFLDAAVFKTFIKTKAQLSTGNEIHAKLNPVSLNA
                     SIGYTF"
     gene            complement(3647..>3956)
                     /locus_tag="VF453_15275"
     CDS             complement(3647..>3956)
                     /locus_tag="VF453_15275"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEX7689075.1"
                     /translation="LLQWDWRSPAMPELASSLPLNGVDGTLRHAGSTPGRAHLKTGSM
                     RDVAAIAGYVLADTGRRYVLVAIVNDPNAAAAHPALDAAVDWVIHDPANPAPPAAATP
                     "
BASE COUNT          631 a         1484 c         1294 g          547 t
ORIGIN      
        1 gctcacgcgg gcgcaactcg aggcggtgct gggcggcccg ctgcgcgccc cgggcgacga
       61 cgcgccgcgc gcctccggca ccctcgaggc gcactacgcg ccgcgcgcca agctgcgcct
      121 gatgccggcg gcgcagctgc gcaccgcgct gcaggtgctg ccgaaatcga ccgttaaccc
      181 ggtggcggta tattcccgca cggtcgcgcc gggcccgtcc gtgcgggcgt cgcgctccat
      241 gcccgacgag cccatcgccg cggcgcacga gctcttcgcc gcgctgcgcg agctcgacgc
      301 ctgcggcgcc agcctcatct gggtcgagac gccgcccgac ggcccggcct gggagggcgt
      361 gcgggaccgg ctgcagcgcg cggccgccgc ctgacccgcc agatcccgat cccggccgcc
      421 gcgacgcgcc ccgcccgcga cggccccagc cgaatccaac aggagaacga atgcttttca
      481 agaaccacct gggccgcttc gcgacgcgcg cggcggcggg actggccgcg gcgctcgcgc
      541 tggcgtcgtg cggcggcgga acctaccagg aacacgcgtt cgtgcccgcc cgcatcctga
      601 ccttcggcga cgagagcaac tcgctgatcg gcacccaggg cctgaagtac tccatcaacg
      661 gcctcaacgc gcagacgcac cagatcgact gcacggtctc gccgctgtgg acgcaggtgc
      721 tcgcgaactc gtacgcgatc tcctaccagc gctgcaacac ccaggaggtg gcgtcgccgc
      781 agggctacga cttctccacc gtcaacgcga ccgtcgccga caccgccaac caggtccagg
      841 ccttcctcgc gggcgactcg ttcaactcgg acgacctcgt gacgatctgg gtcggcgtgc
      901 acgacatcct cgacgactac gccaccaacg gcgcgaccgc cgccagcggc gacttcagca
      961 gcctgctcaa cgacgcgcac ggcttcgggg tgagcctcgc gcaggtcgtc aaccaggtcg
     1021 cgaactcggg cgccaaggtc gtcctgctca cgctgcccga catgggcgag agcccgttcg
     1081 cctacaacga gaacctgcgc ggcgacttca accgcgccga cctgctgtcg cagatgacgc
     1141 agaagttcaa cgacggcctg cgcgccaaca tcgtcaacga cggcagcaag atcggcctcg
     1201 tcctcgtcga cgacttcgtg cgcaacgcgg tgcgcagccc gggctcgtac ggcctgatca
     1261 ccaatccctt cgcggaagcc ggctgcaagg cgagcgtcgc gctgcccgac tgcaccgacg
     1321 acgacaccgt caccgacgcg ggcaagaaca gcaccctgcc gtcgatcttc ctgtgggccg
     1381 acgcgaccca cctggcgccg accgcgcatg cgcagatcgg cagcgaggcc gtctctcgcg
     1441 cgcacacgaa cccgttctga acgcgcgcgc gacgcggcgc cctggcggcg tcgctttcgc
     1501 gccgaccgtg gccctgccgc gacgctcgcg tcggcagggc ctttttcttg ccctgtcgcc
     1561 gacgcgtcct cgcttaacgt gaactagtcc ctcgccgaag tgttgcatct tccgcggtcg
     1621 acagggtttg tacccaaggc cggccaaagc tcgctgcgta tagttttttg cggaaatcga
     1681 acggtcgttc gataactttt ctctctgctt tcggatggcg aggtgcacat gaagacattc
     1741 cacgttctga ccacggccct ggcggctgcg gccttcctgg ccggttgcgg cggcaacgat
     1801 gccggcgacc agtcccctcg cgtcgcgttc aagacgatgg tcaatttcgg cgacagcctg
     1861 agcgacgtcg gcacctacga cgtcggcgcc gtggcgctgc agggcggcgg acactactcg
     1921 atcaacggca agggcgcgac cggcctggcc tacatcaact ggacggagtt cctcgccgcc
     1981 acgctgcaat tgccgcagcc ctgcgccgcg gagacgggcc tggacacgat ccaggccgtg
     2041 atcggcgtgc ccgccgtgcc ggcgaccttc cacgacaccg gggccacgcc ctgctacgac
     2101 tacgcccagg gcggcgcgcg cgtgaccgcg gacgtcggtc ccggcaacgc cgcgctgttc
     2161 aacgcgcagg accagagcac ctacagcgac gccatcggcc agctgacggt gccggtgaag
     2221 acgcagatcg ccaactacac gctgcacggc ggcaccttcg acggcgacga caagctcttc
     2281 accgtcttcg cgggcggcaa cgacctgttc atgcaggtcg gcatcatcca ggcgacgccg
     2341 gtggcgcagc aggccgcggc ggcccaggcc gcgggcgtcg cgatggccac cgcgggcgcc
     2401 gaggaggctg cctacgtgaa tcagatgatc gcggccggcg ccaagcacgt cgtcgtcgtc
     2461 ctgctgccgg acgtgagcct gacgccctac gccatcagcg ccggcgcgac cggccaggcg
     2521 ctcgtgctgc agctgacgca ggccttcaac gcgcagctca ccaagggcct gcaggcgagc
     2581 gactcgctgc tggtcgtcga cgccttctcg gcgagccagg accaggccgc gcacatgtcg
     2641 cagtacggcc tgatcaacgt gacggcgccg gcatgcacga gcagcagctc gctggtgtgc
     2701 accgccaaca cgctggtctc gacgcccgac gacctcgccg ccgatcccac caaggcgaac
     2761 ggctaccagt acgccgactc ggtgcacccg acgccctacg gctacaagct gcttgcccag
     2821 ctcgtcggcg agcagatggc catcaagggc tggctgtaag ccggcgccgc tgcaacactg
     2881 caaggacaca cgttcatgaa gaagcactcc gttgcctcga tcctcgtcgc cgccgccgcc
     2941 ctggcggccg cgggcggcgc gcaagcccag caagccggca ccttctcggt caaggtgggc
     3001 tggaacaaga tcatgcccaa ggtgaagagc gacgacctgt cggagccttc gctgcccggc
     3061 tcgaagatcg acatccactc ggccagcgcg gcgatcatca cggccagcta catgttcacc
     3121 gacgccatcg gcatcgaggc gctgggcggc ctgccgtaca agcacaacat ctacgccgcc
     3181 ggcgcggtcg agggcgtggg caagatcggc agcatccacc agatctcgcc gaccctgctg
     3241 ctcgagtacc gcctgctcgc cgccgacgcc ccgttccggc cgtacgtcgg cgccggcccg
     3301 accttcgcca agttctacgg caccaagggc tcggcgcagc tgacggcggt gaccaacccg
     3361 ggcggctcgc cgacgacgat cggcagcgac accgagtggg gcggcaccct ggccgcgggc
     3421 ggccgctacc gcatcgacag ccactggttc ctcgacgccg cggtcttcaa gaccttcatc
     3481 aagacgaagg cgcagctgtc gaccggcaac gagatccacg ccaagctgaa ccccgtgtcg
     3541 ctcaacgcgt cgatcggcta caccttctga tggcggtcgg cggccgtgcg gtcgccgcac
     3601 ccgctcgagg caggaatccc gcgcgccgcg aggcccgcgg gatttctcat ggcgtcgcgg
     3661 cagccggcgg cgccgggttc gccgggtcgt ggatgaccca gtcgaccgcc gcgtccagcg
     3721 cggggtgcgc ggcggccgcg ttcgggtcgt tgacgatcgc caccagcacg tagcggcggc
     3781 cggtgtccgc gagcacatag ccggcgatcg ccgcgacgtc gcgcatcgag cccgtcttca
     3841 ggtgggctcg gcccggcgtc gagccggcgt ggcgcagcgt gccgtcgacg ccgttgagcg
     3901 gcagcgagct cgcgagctcg ggcatcgccg ggctgcgcca gtcccactgc agcagc
//
LOCUS       DASVLN010000642         5949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_518871, whole genome shotgun sequence.
ACCESSION   DASVLN010000642 DASVLN010000000
VERSION     DASVLN010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518871"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VF453_15280"
     CDS             complement(<1..467)
                     /locus_tag="VF453_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7689076.1"
                     /translation="MTATIQPRLPATFTAARVSRIDGPFELVELPLPGLRPGHVLVRI
                     RAAGVSPLDTKIRAGKAAHARQPLPAVLGMDLAGEVVAVGAGVTRLAVGDEVYGLAGG
                     IGGLQGTLAEYAAIDADLLARKPASLSMREAAALPLNAITAWEGLVDRANVRAG"
     gene            566..1522
                     /locus_tag="VF453_15285"
     CDS             566..1522
                     /locus_tag="VF453_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7689077.1"
                     /translation="MTIAMDWSDVRIFLAVARAGSLSGAAQRLGLSQPTMGRRLRALE
                     DAVGQALFQRGREGFTLTDEGSAVLAHAQRMEDEALAIERALAGHGQALQGVLRVSAS
                     DWFGAHLLTPVCAEFMRRHPQVHVELLTDARVLDLARREADLVFRIKPPDDAELVRRR
                     LMTMEYALYGPEGVPPPLPGDGAGTTLVTLDAAYEALPDVAWLKKTFPRARLGFGSNS
                     REAQARMVALGAGFAVLPVRLASRIPGLVAHDVAGTPPSREIWAAYHRDLRGLARLRS
                     FLDLAVELLGPRPATATATATATAMAVATAGTKPVNPRPRAR"
     gene            complement(1497..3386)
                     /locus_tag="VF453_15290"
     CDS             complement(1497..3386)
                     /locus_tag="VF453_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689078.1"
                     /translation="MLAYFERLIDPYPDAPAVPPPQGLVRFIWACSRGARRYVIGMML
                     LTAIIGVFEAWLFAVLGHVVDWLSHVEPARMWAEQRGRLLALAAVLALSPALVSVQAM
                     LKYQTIFANFPMRLRWNFHRLMLAQSMGFYQDEFAGRVATKVMQTALGVREVMMTLTD
                     ILVFVVIYFATMVAVVGGFDLWLLGPFLGWMTLYAIALKYFVPRLGRIGAAQADARSL
                     MTGRVTDAYTNIATVKLFSHARREASYAREAMQEFMLTAYGQGRYITGIEIVNHTLSM
                     GLIMSTCGVTLWLWTLGKVGVGAVAASTALALRLNGISHWIMWEMASLFENIGTVTDG
                     MRTLSRPHAIVDAPGAPELAVTRGEVRFEDVRFSYGGKRQVIDRFSLTIRPGEKIGLV
                     GRSGAGKSTLVNLLLRFYDVESGRVLVDGQDISKVTQNSLRAHVGMVTQDTSLLHRSV
                     RDNILYGRPDADEAQMVAAARRAEAEDFIAGLADAKGRKGYDAHVGERGVKLSGGQRQ
                     RIAIARVMLKDAPILLLDEATSALDSEVEAAIQASLYSLMEGKTVIAIAHRLSTIAAM
                     DRLVVLDKGRIVEEGDHRSLLARGGLYARLWAHQSGGFLGEEVDQDAPPQRADQLVTE
                     PEAED"
     gene            complement(3509..4405)
                     /locus_tag="VF453_15295"
     CDS             complement(3509..4405)
                     /locus_tag="VF453_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX7689079.1"
                     /translation="MKWEGQRQSDNVEDRRDEDYGGGGGGGFGLPHISIAGIVIAVVA
                     GLIFHVNPLVILGLMSGGGQVTQQQSAPQPTHVQRQHTPEEERKVAFVKTVLADTEDV
                     WTQVFKERGQAYRDPKLVLFRNAIPTACGAGEAAMGPFYCPGDEKVYIDLSFYDLLQT
                     KLGAPGEFAEAYVIAHEVGHHVQKLLGISGKVDAARRNADRVRSNALSVRLELQADCF
                     AGVWANRAQRMKQILEQGDIESALNAASQIGDDTLQRKARGTVVPETFTHGTSAQRVH
                     WFRQGFDGGDPQQCDTFRAPQL"
     gene            complement(4542..>5949)
                     /locus_tag="VF453_15300"
     CDS             complement(4542..>5949)
                     /locus_tag="VF453_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7689080.1"
                     /translation="ADATDPDALATIQDTGARDTIPAITLDQAAQSAAASGARVYAVG
                     GSSAPAGATHVATMAAVPWASLPAGSAVLVAAGTYAGVTTISAAGTQAAPITITAADP
                     AHPPVFTNSFDFQHASHVHLSHVTVQSPAYAGVIVRQGSDHVVVSDSTIRNAPVGLTI
                     TDGAGTGHQFLRNLITASFAHGISVEVNADPAERTLIAHNTITGSGQHGMEIRASHYQ
                     VEYNTVAGSGAGGGGGVSGIHVYSGSPTEDSGDDNLVRYNLAYGSLDPVAFDGNGIEV
                     DQWCDGNTVAYNQVWGNDGAGIIVYDGSGNLVHNNTAWGDGLDSGHTHVARGEIIVSG
                     TAAANVHGNHVWNNVASSTTAVVPALYVDSRAVAGGNTIGANLYWNAASGGLVLRWTD
                     GLFRQTAALIDASTGVPGSVVAAPAFANTTAKLSNGLRLTKAPALPGLMPAGQSDYAG
                     VAAAAGQVWLGAYFTPAN"
BASE COUNT          783 a         2117 c         2177 g          872 t
ORIGIN      
        1 ccggcgcgca cgttcgcgcg gtcgacgagg ccctcccacg ccgtgatcgc gttcagcggc
       61 aacgccgcgg cctcgcgcat cgacagcgac gcgggcttgc gggcgagcag gtcggcgtcg
      121 atggccgcat attcggcgag cgtgccctgc agcccgccga tgccgccggc caggccgtag
      181 acctcgtcgc cgacggccag gcgcgttacg cccgcgccga ccgcgacgac ctcgcccgcc
      241 aggtccatgc cgagcaccgc cggcagcggc tgcctggcgt gcgcggcctt gccggcgcgg
      301 atcttcgtgt cgagcgggct gacgccggcg gcgcggatgc gtaccagcac gtggccgggg
      361 cgcagtccgg gcaacggcag ctcgacgagc tcgaacgggc cgtcgatgcg cgagacgcgc
      421 gcggcggtga aggtggcggg cagtcggggc tggatcgtgg cggtcatggc gatgtcctcg
      481 ggtgggttga agcgacggcg ccagtttgtg atgttcagga tcgcatgtaa attcacgatt
      541 ccgaacactt cgcattcaat atcgaatgac gatcgccatg gactggagcg acgtgcgcat
      601 cttcctggcc gtcgcgcgcg cgggctcgct cagcggcgcg gcgcagcggc tggggctctc
      661 gcagccgacg atgggccggc gcctgcgcgc actcgaggac gccgtcggcc aggccctgtt
      721 ccagcgcggg cgcgagggct tcacgctgac cgacgaaggc tcggccgtgc tcgcgcacgc
      781 gcagcgcatg gaggacgagg cgctcgccat cgagcgcgcg ctcgcgggcc acggccaggc
      841 gctgcagggc gtgctgcgcg tctcggcctc ggactggttc ggcgcgcacc tgctgacgcc
      901 cgtgtgcgcc gagttcatgc gtcgccatcc gcaggtgcac gtcgagctgc tgaccgacgc
      961 ccgcgtcctc gacctcgcgc gccgggaggc cgacctcgtc ttccgcatca agccccccga
     1021 cgacgccgaa ctcgtgcggc gccggctgat gaccatggag tacgcgctct acgggccgga
     1081 gggcgtgccg ccgcccctgc ccggcgacgg cgccggcacg acgctcgtca cgctggacgc
     1141 cgcctacgag gcgctgcccg acgtggcctg gctgaagaag accttcccgc gcgcccgcct
     1201 gggcttcggc agcaacagcc gcgaggccca ggcgcggatg gtcgcgctcg gggcgggctt
     1261 cgcggtgctg ccggtgcggc tcgcgagccg gatccccggc ctcgtcgcgc acgacgtcgc
     1321 cggcacgccg ccgtcgcgcg agatctgggc cgcgtaccac cgcgacctgc gcgggctcgc
     1381 gcggctgcgc tcgttcctcg acctcgcggt ggagctgctg gggccgcggc ctgcgacggc
     1441 gacggcgacg gcgacggcga cggcgatggc ggtggcgacg gcgggcacga agcccgtcaa
     1501 tcctcggcct cgggctcggt gacgagctgg tcggcgcgct gcggcggcgc gtcctggtcg
     1561 acctcctcgc ccaggaagcc gccgctctgg tgcgcccaca ggcgcgcgta caggcccccg
     1621 cgcgcgagca ggctgcgatg gtcgccctcc tcgacgatgc ggcccttgtc gaggacgacc
     1681 aggcgatcca tcgcggcgat ggtcgacagc cggtgcgcga tcgcgatgac ggtcttgccc
     1741 tccatcagcg agtacaggct cgcctggatc gcggcctcga cctcggagtc gagcgcgctc
     1801 gtcgcctcgt ccagcagcag gatgggtgcg tccttcaaca tgacgcgcgc gatggcgatg
     1861 cgctggcgct ggccgcccga gagcttgacg ccccgctcgc cgacgtgcgc gtcgtagccc
     1921 ttgcggccct tggcgtcggc caggccggcg atgaagtcct cggcctcggc gcggcgcgcg
     1981 gcggcgacca tctgggcctc gtccgcgtcg ggccggccgt agaggatgtt gtcgcgtacg
     2041 gagcggtgca gcagcgaggt gtcctgggtc accatgccga cgtgggcgcg caggctgttc
     2101 tgcgtcacct tcgagatgtc ctggccgtcg accagcaccc ggccggactc gacgtcgtag
     2161 aagcgcagca gcaggttcac gagcgtcgac ttgcccgccc ccgagcgccc gacgaggccg
     2221 atcttctcgc ccggccggat cgtcagcgag aagcggtcga tcacctggcg cttgccgccg
     2281 tacgagaaac ggacgtcctc gaagcgcacc tcgccgcgcg tcacggccag ctcgggcgcg
     2341 cccggcgcgt cgacgatcgc gtgcggccgc gacagggtgc gcatgccgtc ggtgacggtg
     2401 ccgatgttct cgaacagcga cgccatctcc cacatgatcc agtgcgagat gccgttgagc
     2461 cgcagcgcga gcgccgtgga tgccgccacc gcgccgacgc cgaccttgcc cagcgtccac
     2521 agccacagcg tgacgccgca ggtgctcatg atgaggccca tgctcagcgt gtggttcacg
     2581 atctcgatgc cggtgatgta gcggccctgg ccgtaggccg tgagcatgaa ctcctgcatc
     2641 gcctcgcgcg cgtagctcgc ctcgcggcgc gcgtgcgaga acagcttgac ggtggcgatg
     2701 ttggtgtacg cgtccgtcac gcggcccgtc atcagcgagc gcgcgtcggc ctgcgccgcg
     2761 ccgatgcgcc cgagccgcgg cacgaagtac ttgagcgcga tcgcgtacag cgtcatccag
     2821 ccgaggaagg gcccgagcag ccagaggtcg aagccgccga ccaccgccac catggtcgcg
     2881 aagtagatga cgacgaagac gaggatgtcg gtgagcgtca tcatcacctc gcgcacgccc
     2941 agcgccgtct gcatcacctt cgtggcgacg cggccggcga actcgtcctg gtagaagccc
     3001 atgctctgcg ccagcatcag ccggtggaag ttccagcgca ggcgcatcgg gaagttggcg
     3061 aagatcgtct ggtacttcag catcgcctgc accgagacca gcgccgggct cagcgccagc
     3121 accgccgcca gcgccagcag ccggccgcgc tgctcggccc acatgcgggc cggctcgacg
     3181 tggctcagcc agtcgacgac atggcccagc accgcgaaca gccacgcctc gaacaccccg
     3241 atgatggccg tcagcagcat catcccgatc acgtagcgcc gcgcgccgcg cgagcaggcc
     3301 cagatgaagc gcacgaggcc ctgcgggggc ggcacggcgg gcgcgtcggg atacgggtcg
     3361 atcagtcgtt cgaagtaggc gagcaagacg ggggctccgg cgcgccggcg cgggcgggtg
     3421 gcgcgaaacc cgcaaggcta gcggaaatcg acggcgtgcg ccggtccgtt cgcgcatcgg
     3481 tgcgatgcgg ccgccgcatc gatcgcgctc agagctgcgg cgcgcggaag gtgtcgcact
     3541 gctgcgggtc gccgccgtcg aacccctgcc tgaaccagtg cacgcgctgg gcgctggtgc
     3601 cgtgcgtgaa cgtctcgggc acgaccgtgc cgcgggcctt gcgctgcagc gtgtcgtcgc
     3661 cgatctgcga ggcggcgttg agcgcgctct cgatgtcgcc ctgctcgagg atctgcttca
     3721 tgcgctgcgc ccggttggcc cagacgccgg cgaagcagtc cgcctgcagc tccagccgca
     3781 ccgacagcgc gttggagcgc acgcggtcgg cgttgcggcg cgcggcgtcg accttgcccg
     3841 agatcccgag cagcttctgc acgtggtggc cgacctcgtg ggcgatcacg taggcctcgg
     3901 cgaactcgcc tggcgcgccg agcttggtct gcagcaggtc gtagaagctc aggtcgatgt
     3961 agaccttctc gtcgcccggg cagtagaacg gccccatcgc ggcctcgccg gcgccgcagg
     4021 cggtggggat ggcgttgcgg aagagcacga gcttcgggtc gcggtaggcc tggccgcgct
     4081 ccttgaagac ctgcgtccac acgtcctcgg tgtcggcgag cacggtcttc acgaacgcga
     4141 ccttgcgctc ctcctcgggc gtgtgctggc gctgcacgtg ggtcggctgc ggcgccgact
     4201 gctgctgcgt cacctgcccg ccgccgctca tcagcccgag gatcaccagc gggttcacgt
     4261 ggaagatcaa ccccgccacc accgcgatca cgatgcccgc gatgctgatg tgcggcagcc
     4321 cgaagccgcc cccgccgccg ccgccgtagt cctcgtcgcg gcgatcctcc acgttgtcgc
     4381 tctggcgctg gccttcccac ttcatcgcgt tgctcctggc gcgcccgtcc gcggggcgcg
     4441 gagggcatcg ggcgtgattg tcggcgaatc gggcaccggg gcgcgctccg cggacgcgaa
     4501 ggcggacggg tgcccggcgg cgggcggccc tcggcgcgcg atcagttcgc gggcgtgaag
     4561 tacgcgccga gccacacctg gccggcggcc gcggcgacgc cggcatagtc cgactgccct
     4621 gccggcatca ggcccggcag cgcgggcgcc ttcgtcaggc gcaggccatt ggacagcttc
     4681 gccgtcgtgt tggcgaaggc cggcgccgcg acgacgctgc cgggcacgcc cgtgctcgcg
     4741 tcgatgagtg cggcggtctg cctgaaaagg ccgtccgtcc agcgcagcac gagcccgccg
     4801 gacgcggcgt tccagtacag gttggcgccg atcgtgttgc cgcccgcgac cgcccgcgag
     4861 tcgacgtaga gcgccggcac gaccgcggtc gtcgaggacg cgacgttgtt ccagacgtgg
     4921 ttgccgtgca cgttcgccgc cgcggtgccg ctgacgatga tctcgccgcg cgcgacatgc
     4981 gtgtggccgc tgtccaggcc gtcgccccag gccgtgttgt tgtgcaccag gttgccgctg
     5041 ccgtcgtaga cgatgatgcc cgcgccgtcg ttgccccaga cctggttgta ggcgaccgtg
     5101 ttgccgtcgc accattggtc gacctcgatg ccgttgccgt cgaaggcgac cgggtcgagg
     5161 ctgccgtagg cgaggttgta gcgcacgagg ttgtcgtcgc cgctgtcctc ggtcgggctg
     5221 ccgctgtaga cgtggatgcc cgagacgccg ccgccgccgc ccgccccgct gccggccacg
     5281 gtgttgtact cgacctggta gtgcgaggcg cggatctcca tgccgtgctg gccgctgccc
     5341 gtgatcgtgt tgtgcgcgat gagcgtgcgc tcggccgggt cggcgttcac ctcgacgctg
     5401 atgccgtggg cgaagctggc cgtgatcagg ttgcgcagga actggtggcc ggtgccggcg
     5461 ccgtcggtga tcgtcaggcc gaccggcgcg ttgcggatcg tcgagtcgga cacgacgacg
     5521 tggtcgctgc cctggcgcac gatgacgccg gcgtaggcag gcgactgcac cgtcacgtgc
     5581 gacagatgca catgagaggc gtgctggaag tcgaagctgt tggtgaagac cggcggatgc
     5641 gccgggtcgg cggccgtgat cgtgatcggg gcggcctgcg tcccggcagc ggagatcgtg
     5701 gtgacgcccg cgtacgtgcc cgcggccacg agcaccgcgc tgcccgcggg cagcgaagcc
     5761 cagggcaccg ccgccatcgt ggccacgtgc gtcgcgcccg ccggtgccga gctgccgccg
     5821 accgcataca cccgggcacc gctggcggcc gccgactgcg ccgcctggtc aagcgtgatc
     5881 gccgggatcg tgtcgcgcgc gccggtgtcc tggatcgtgg cgagcgcgtc agggtcggtc
     5941 gcgtcggcg
//
LOCUS       DASVLN010000643         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_194510, whole genome shotgun sequence.
ACCESSION   DASVLN010000643 DASVLN010000000
VERSION     DASVLN010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194510"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VF453_15305"
     CDS             <1..733
                     /locus_tag="VF453_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7689081.1"
                     /translation="VAAAAAVALVAGHAVPLWLQGLLTLALVTATGPLIYRLAYQPLA
                     DASVLLLLIVSVGVHFALVGLGLVFFGAEGYRTPPFWDTSFALGPLTLTGQTIVVLAA
                     SAALMAALALFFGRTLAGKALRATAINRLGARLVGVSTEHAGRSAFAMAAFVGALAGL
                     LIGPSTTMIYDSGFLIGLKGFVAAIIGGLSSYPVTVVGALLVGLLDSFSSFWASAYKD
                     AIVFAAIVPVLLLRSLTDRRHDEDE"
     gene            717..>1728
                     /locus_tag="VF453_15310"
     CDS             717..>1728
                     /locus_tag="VF453_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7689082.1"
                     /translation="MTKTSDAVTPGRAALGAGLVLLFALPALPVPEFWITQLDYIGLY
                     ALVVLGLVLLTGVAGLTSFGQAAFVGLGAYATAWTTTALGVSPWIGLACGLALTVAVA
                     LVLAGVTLRMSGHYLPLATIAWGLALYFLVGNLGFLGKFDGLQGIPPVRVAGAELRTG
                     RASYELIWAFAVLGAFALRHLLDSRAGRALRALKGGTVMAEAMGVATLRYKVLAFLVA
                     ALYASVSGWLFAHYQRSVNPSPFGLQMGIEYLFMAVLGGVGLVGGAFVGAGAVKVAED
                     RLQDWLPALLGTSGPVELVVFGMVLVLVLQVTPDGLWSLVTRRRRGRRHDPAARADAE
                     PLA"
BASE COUNT          158 a          631 c          635 g          304 t
ORIGIN      
        1 ggtcgccgcc gcggcggccg tggcgctcgt ggccggccac gccgtgccgc tgtggctgca
       61 gggcctgctg acgctggcgc tggtgacggc caccggcccg ctgatctacc gcctcgccta
      121 ccagccgctg gccgacgcct cggtgctgct gctgctgatc gtctcggtgg gcgtgcattt
      181 cgcgctcgtc ggactggggc tcgtgttctt cggcgccgag ggctaccgca cgccgccgtt
      241 ctgggacacg tcgttcgcgc tcgggccgct cacgctgacc ggccagacga tcgtcgtgct
      301 cgcggcctcc gcggcgctga tggcggcgct cgccctcttc ttcggccgca cgctggccgg
      361 caaggcgctg cgggccaccg cgatcaaccg gctgggcgcg cggctggtgg gcgtgtccac
      421 cgagcacgcc ggccgcagcg cgttcgcgat ggccgccttc gtcggcgcgc tggcgggcct
      481 gctgatcggg ccgtcgacga cgatgatcta cgacagcggc ttcctgatcg gcctcaaggg
      541 cttcgtcgcc gcgatcatcg gcggactctc cagctatccg gtgaccgtcg tcggcgcgct
      601 gctcgtgggc ctgctcgact ccttcagctc cttctgggcc agcgcgtaca aggacgcgat
      661 cgtgttcgcc gcgatcgttc cggtgctgct gctgcgctcg ctcaccgacc gccgccatga
      721 cgaagacgag tgacgccgtg acccccggcc gcgccgcgct cggcgccggc ctcgtgctgc
      781 tgttcgcgct gccggcgctg cccgtgcccg agttctggat cacgcagctg gactacatcg
      841 gcctgtacgc gctggtcgtc ctcggcctcg tgctgctgac gggggtcgcc ggcctgacct
      901 ccttcgggca ggcggccttc gtcggcctcg gcgcgtacgc cacggcctgg acgacgacgg
      961 cgctcggcgt ctcgccgtgg atcggcctgg cgtgcggact cgcgctgacg gtggccgtcg
     1021 cgctcgtgct cgcgggcgtg acgctgcgca tgtcgggcca ctacctgccg ctggcgacga
     1081 tcgcgtgggg cctcgcgctg tacttcctgg tcggcaacct cggcttcctc ggcaagttcg
     1141 acggcctcca aggcatcccg ccggtgcgcg tcgcgggcgc ggagctgcgc accgggcgcg
     1201 catcctacga gctgatctgg gccttcgcgg tgctcggcgc attcgcgctg cgccacctgc
     1261 tggattcccg cgcaggccgc gcgctgcgtg cgctcaaggg cggcaccgtg atggccgagg
     1321 cgatgggcgt ggccacgctg cgctacaagg tgctcgcgtt cctcgtcgcg gcgctgtatg
     1381 cgtcggtctc gggctggctg ttcgcgcact accagcgctc ggtcaacccc tcgcccttcg
     1441 ggctgcagat gggcatcgag tacctgttca tggcggtgct gggcggcgtc ggcctcgtgg
     1501 gcggcgcctt cgtcggcgca ggcgcggtca aggtcgcgga ggaccggctg caggactggc
     1561 tgcccgcgct gctgggcacg agcggcccgg tggagctggt cgtcttcggc atggtgctgg
     1621 tgctggtgct gcaggtcacg cccgacgggc tgtggtcgct cgtcacgcga cggcggcgtg
     1681 ggcgccgcca cgatcccgcg gcccgggccg acgccgagcc gctcgcgc
//
LOCUS       DASVLN010000644         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_87529, whole genome shotgun sequence.
ACCESSION   DASVLN010000644 DASVLN010000000
VERSION     DASVLN010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87529"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..>2146
                     /locus_tag="VF453_15315"
     CDS             353..>2146
                     /locus_tag="VF453_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7689083.1"
                     /translation="MAPRVSKILIVDDTPHNIDVLSATLADEPCELMAATSGARALEL
                     AARGRPDLVLLDVMMPGMDGFEVCRRLKADPATADLPVVFVTARTDDVSLGFAAGGAD
                     YISKPINADEVRARVRYQLERRMLLEQLRELNQGLEEKVRERTAELTIANRHLRQEIN
                     ERRYMQDRLNYLATHDFVTRLYNRGALDAHVSELLARVQRSEAEAVFLLIDIDQFRLV
                     NETCGCIAGDELLRQFAEVVGGLLDRTDFFARLGGDKFGVVSENANADAGAALARRIV
                     AQLTEFEFIWEERSFKMAASVAVVRIGPDIVSFDQLMLTADEAAYLAKRDGRGTIRVH
                     DASVLDAEGHRESVNWALVLVDALKHRHFRAFFQRLQPLAAADAGQAPGLHVETLIRL
                     WDPRRQKLVLPSQFIAPAERFHLVGELDRWMIREVVSLLGGLAESHEHIGQVAVNLSA
                     VSIREPGLAQFVAETLAEFHVPGRLLCFEITETEAIVNLQSAGDFMHALHKLGCRFSL
                     DDFGSGFASFTYLRQLPFDSIKIDGMFVRDMDGDLVHGGMVRSMAEMARLLKKPVVAE
                     CVETAAVAAMLRELGVEWAQGFHFHRPEPLTV"
BASE COUNT          309 a          757 c          753 g          327 t
ORIGIN      
        1 ggtgcgtcgc ctggtcgcgg cgctcgcgcc cgaggcggtc ggacgcgcgc gctggcgcgg
       61 tttcgacgag gcggcccagg cctacgactt ccagcagctc gaggcgcgcg tgcgcgagct
      121 gctcgcgcag gcccaggatg ggtgaggatc cgcggcccgg acggccgcgt cgtcgcccat
      181 ccgggccggg gagcatagtg ttgaccctgt actgcgggtg aacccgcgaa gcgggcgtgc
      241 gacattgcac gaacggcggc gtcctggcga ttcgccgtcg ccccggtgtg tttaaactgc
      301 ggcgagggtg ggtttccagt gtgatcccga ccaaagccag gagagcgcgg ttgtggcccc
      361 tagagtctcg aaaatcctga tcgtcgacga cacgccgcac aacatcgacg tgctcagcgc
      421 gacgcttgcg gatgaaccgt gcgagctgat ggccgcgacc tcgggcgccc gggcgctgga
      481 gctcgccgcc cgcggccgcc ccgacctcgt gctgctggac gtgatgatgc ccggcatgga
      541 cggcttcgag gtgtgccgcc gcctgaaggc cgatcccgcg acggccgacc tgccggtggt
      601 cttcgtcacc gcccgcaccg acgacgtgag cctcggcttc gccgccggcg gcgccgacta
      661 catctccaag ccgatcaacg ccgacgaggt gcgcgcccgg gtgcgctacc agctcgagcg
      721 tcgcatgctg ctggagcagc tgcgcgagct gaaccagggc ctcgaggaga aggtgcgcga
      781 gcgcaccgcc gaactcacga tcgccaaccg ccacctgcgc caggagatca acgagcggcg
      841 ctacatgcag gaccggctca actacctggc cacgcacgac ttcgtcaccc gcctgtacaa
      901 ccgcggcgcg ctcgacgccc acgtctcgga gctgctcgcc cgcgtgcagc gcagcgaggc
      961 cgaggccgtc ttcctgctga tcgacatcga ccagttccgc ctcgtcaacg agacctgcgg
     1021 ctgcatcgcc ggcgacgagc tcctgcgcca gttcgccgag gtggtgggcg gcctgctcga
     1081 tcgcaccgac ttcttcgcgc gcctgggcgg cgacaagttc ggcgtcgtca gcgagaacgc
     1141 gaacgccgac gccggcgccg cgctcgcgcg ccgcatcgtc gcgcagctga ccgagttcga
     1201 gttcatctgg gaggagcgca gcttcaagat ggccgcctcg gtggccgtcg tgcgcatcgg
     1261 accggacatc gtctcgttcg accagctgat gctcaccgcc gacgaggccg cctacctcgc
     1321 caagcgcgac ggccgcggca cgatccgggt gcacgacgcc tcggtgctcg acgccgaggg
     1381 ccaccgcgag tcggtcaact gggcgctggt gctggtcgac gcgctgaagc atcgccactt
     1441 ccgcgccttc ttccagcgcc tgcagccgct cgccgcggcc gatgccggcc aggcgccggg
     1501 cctgcacgtc gagacgctga tccgcctgtg ggatccgcgc cggcagaagc tggtgctgcc
     1561 gtcgcagttc atcgcgcccg cggaacgctt ccacctggtc ggcgagctcg atcgctggat
     1621 gatccgggag gtcgtctcgc tgctgggcgg cctggccgag agccacgagc acatcgggca
     1681 ggtggcggtg aacctgtcgg cggtgtcgat ccgcgagccc gggctcgcgc agttcgtggc
     1741 cgagacgctg gcggagttcc acgtcccggg ccgcctgctg tgcttcgaga tcaccgagac
     1801 cgaggcgatc gtcaacctgc agagcgcggg cgacttcatg cacgcgctgc acaagctggg
     1861 ctgccgcttc tcgctcgacg acttcggcag cggcttcgcg tcgttcacct acctgcgcca
     1921 gctgccgttc gactcgatca agatcgacgg catgttcgtg cgcgacatgg acggcgacct
     1981 cgtgcacggg ggcatggtcc gctcgatggc cgagatggcg cggctgctca agaaaccggt
     2041 ggtggccgaa tgcgtcgaga cggcggccgt ggccgcgatg ctgcgcgagc tgggtgtcga
     2101 gtgggcgcag ggcttccact tccaccggcc cgagccgctg acggtc
//
LOCUS       DASVLN010000645         1513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_464882, whole genome shotgun sequence.
ACCESSION   DASVLN010000645 DASVLN010000000
VERSION     DASVLN010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464882"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1513
                     /locus_tag="VF453_15320"
     CDS             <1..>1513
                     /locus_tag="VF453_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689084.1"
                     /translation="GDAPAAAAALRRSCLGVFAVLLLVGGVAGWWAVLARQSRRVAQE
                     ESNRQTQALGEQAVLLQREIDSHRRTDAELQRAKAAADAANQAKSRYISTLSHELRTP
                     LNSVIGYAQLLDDDPALPAHRRQAVGVIRRGGEHLLSLIEGTLDLARIEGGKVSLAIA
                     AVPLRDLLEQLADMFALQARAKGLRFVAEVDDGLPRAVRADERKLRQVLINVLGNAVK
                     FTDRGEVRLRVRHEREIAQFDIEDTGPGIAPADLERVFEPFARGAAIAGASPGTGLGL
                     TIAKMLTELMGGEMTVRSAPGAGTAFRIRLFLPAQFVAGVPAGPAPRRIGYRGARRRV
                     LAVDNEEVDRELLRQLLVPLGFEVEVAGSGEEALARLRTGVRDGWRPDAILVDLAMPG
                     IDGWETIRTLRSQRLSDAPVAIVSANAFDRRLDNDLGLGADDYVLKPVRGDELLAWLG
                     RALAIDWCTDADADTAPRIIGEPVATPPRDGPLPAAAALRALAEQVRLGYPRG"
BASE COUNT          186 a          559 c          579 g          189 t
ORIGIN      
        1 tgggcgacgc gccggccgcc gcggcggcgc tgcggcgctc ctgcctgggc gtgttcgcgg
       61 tgctgctgct ggtcggcggc gtcgcgggct ggtgggcggt gctcgcgcgc cagagccgcc
      121 gcgtcgcgca ggaggagagc aaccgccaga cccaggcgct cggcgagcag gcggtgctgc
      181 tgcagcgcga gatcgactcc catcgccgca ccgacgccga actgcagcgc gccaaggccg
      241 cggccgacgc ggccaaccag gccaagagcc gctacatctc gacgttgagc cacgagctgc
      301 gcacgccgct gaacagcgtc atcggctacg cgcagctgct cgacgacgat ccggccctgc
      361 ccgcgcaccg gcgccaggcg gtcggcgtga tccggcgcgg cggcgagcac ctgctgtcgc
      421 tgatcgaggg cacgctggac ctcgcccgca tcgagggcgg caaggtctcg ctcgcgatcg
      481 cggcggtgcc gctgcgcgac ctgctcgagc agctcgcgga catgttcgcg ctgcaggcgc
      541 gcgccaaggg cctgcgcttc gtggccgagg tcgacgacgg cctgccgcgg gcggtgcggg
      601 ccgacgagcg caagctgcgc caggtgctga tcaacgtcct cggcaacgcc gtgaagttca
      661 ccgaccgggg cgaggtgcgg ctgcgcgtgc gccacgagcg cgagatcgcg cagttcgaca
      721 tcgaggatac ggggccgggc atcgcgccgg ccgacctcga gcgcgtcttc gagcccttcg
      781 cgcgcggcgc cgcgatcgcg ggcgcgagcc cgggcaccgg cctgggcctg accatcgcca
      841 agatgctcac cgagctgatg ggcggggaga tgaccgtgcg cagcgcgccc ggcgcgggca
      901 cggcgttccg catccgcctg ttcctgccgg cgcagttcgt cgccggcgtg ccggcgggcc
      961 ccgcgccgcg ccgcatcggc tatcgcggcg cgcggcggcg cgtgctggcc gtcgacaacg
     1021 aggaggtcga tcgcgagctg ctgcgccagc tgctcgtgcc gctgggcttc gaggtggagg
     1081 tcgccggatc gggcgaggag gcgctcgccc ggctgcgcac cggcgtccgc gacggatggc
     1141 ggcccgacgc catcctggtc gacctcgcca tgccgggcat cgacggctgg gagacgatcc
     1201 gcaccttgcg ctcgcagcgc ctgagcgacg cgccggtggc catcgtctcc gccaacgcct
     1261 tcgatcgccg gctcgacaac gacctcggcc tcggcgcaga cgactacgtg ctcaagccgg
     1321 tgcgcggcga cgaactgctg gcgtggctcg gccgcgcgct cgcgatcgac tggtgcaccg
     1381 atgcggatgc cgatacggca ccccgtatca tcggcgagcc ggtcgcgacg ccgccgcggg
     1441 acggcccgct gcccgcggcg gcggcgttgc gcgcgctcgc ggagcaggtg cggctcggct
     1501 acccgcgcgg cat
//
LOCUS       DASVLN010000646         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_518993, whole genome shotgun sequence.
ACCESSION   DASVLN010000646 DASVLN010000000
VERSION     DASVLN010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518993"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="VF453_15325"
     CDS             complement(<1..915)
                     /locus_tag="VF453_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7689085.1"
                     /translation="MAAASLTHRHVEVFRAVMTAGSVTGAAAALFTSQPTISRELARM
                     EHVLRLALFDRVRGRLRPTAQAYALFEEVERSYAGLERVADTALRLRQFTQGQLSIAC
                     LPAFAHALLPGACARFQKAHPGVSVSVNPVESPLLEERLAAQAHDLGVTEHDVPAAGG
                     EARLLLELDEVAVLPAGHALLARTRLAAPDFAGQPFVSFHPRDRYRQLVDHLFAEARV
                     DRQLAVETDSAVAVCAMVRHGLGVAIVNPLTALALAGPGGEGLQVRPLAFAIPFRVHA
                     VRPAHRPGNPLVDRFEAALRAECEALGRQ"
     gene            996..2279
                     /gene="lysA"
                     /locus_tag="VF453_15330"
     CDS             996..2279
                     /gene="lysA"
                     /locus_tag="VF453_15330"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7689086.1"
                     /translation="MPDQTLQHPAPAAFTPELLDRLAREHGTPLWVYDAATIRARVER
                     LRPFDTIRFAQKACSNTHLLRLLRDLDVRVDAVSAGEIERALAAGYDARGEDAGIVFT
                     ADVIDDAALALVAAHGIPVNAGSAGMIDQLGAALGARAAGHRIWLRINPGFGHGHNRK
                     TNTGGEHSKHGIWPSDLPAALAAIARHRMRLVGLHMHIGSGVDYAHLARVCDAMAQHV
                     REVVARGHDVEAISAGGGLSIPYRPGDAVVDTAHYFSLWDAARRQLARELGHPLRLEI
                     EPGRFLVAESGAMLAEVRAVKDMGANHFVLCDAGFNDLVRPAMYGGHHAISVVARTLH
                     EGSQAPRPTVVAGPLCESGDVFTQDAGGVVEPRPLPPAQPGDLLVFHDAGAYGASMSS
                     NYNSRPLAAEVLVDGDQARRIRRRQTVQELIALEN"
BASE COUNT          329 a          887 c          857 g          278 t
ORIGIN      
        1 ctgccgcccc agcgcctcgc actcggcccg cagcgcggcc tcgaagcggt cgaccagcgg
       61 gttgccgggc cggtgcgcgg ggcgcacggc gtggacgcgg aacgggatcg cgaaggcgag
      121 cggacgcacc tgcaggccct cgccgccggg cccggcgagc gcgagcgccg tcagcggatt
      181 gacgatggcc acgcccagcc cgtggcgcac catcgcgcag acggcgacgg cgctgtcggt
      241 ctccacggcg agttggcggt cgacccgggc ctcggcgaag aggtggtcga ccagctggcg
      301 gtagcggtcg cgcgggtgga agctcacgaa gggctggccg gcgaaatccg gggccgccag
      361 gcgcgtgcgc gcgagcagcg cgtggcccgc cggcagcacg gccacctcgt cgagctccag
      421 cagcagccgc gcctcgccgc ccgccgcggg cacgtcgtgc tcggtgacgc ccaggtcgtg
      481 cgcctgggcg gccaggcgtt cctccagcag cggcgactcg accgggttca ccgacacgct
      541 caccccggga tgcgccttct ggaagcgcgc gcacgccccc ggcagcagcg cgtgcgcgaa
      601 cgcgggcagg caggcgatcg acagctgccc ctgcgtgaac tggcgcaggc gcagcgcggt
      661 atccgcgacg cgctcgagac cggcgtagga gcgttcgacc tcctcgaaca gcgcgtaggc
      721 ctgcgcggtg ggccgcagcc gcccgcggac gcggtcgaac agcgcgagcc gcagcacatg
      781 ttccatgcgc gcgagctcgc ggctgatcgt cggctgcgag gtgaacagcg cggccgcggc
      841 gccggtgacg ctgccggcgg tcatgacggc gcggaagacc tcgacgtggc gatgggtgag
      901 cgatgcggcg gccatgcgcg aaccatatca aatccgaatg tgatggccaa atcaaagcac
      961 tggactgaaa ccgtgaaccc gcgcacgatg ccgccatgcc cgaccagacc cttcagcatc
     1021 ccgccccggc cgcattcacg cccgaactcc tcgaccgcct tgcccgcgag cacggcacgc
     1081 cgctgtgggt gtacgacgcc gcgaccatcc gcgcccgcgt cgagcgcctg cgcccgttcg
     1141 acaccatccg cttcgcgcag aaggcgtgct ccaacacgca cctgctgcgc ctgctgcgcg
     1201 acctggacgt gcgcgtggac gccgtgtcgg cgggcgagat cgaacgcgcg ctggccgccg
     1261 gctacgacgc gcgcggcgag gacgcgggca tcgtcttcac ggccgacgtg atcgacgacg
     1321 cggcgctcgc gctcgtggcc gcgcacggca tcccggtgaa cgccggctcg gcgggcatga
     1381 tcgaccagct cggcgccgcc ctcggcgccc gcgcggccgg ccatcggatc tggctgcgca
     1441 tcaacccggg cttcggccac ggccacaacc gcaagaccaa caccggcggc gagcacagca
     1501 agcacggcat ctggccgtcc gacctgcccg ccgcgctcgc ggccatcgcg cgccaccgca
     1561 tgcgcctggt cggcctgcac atgcacatcg gctcgggcgt ggactacgca cacctcgcgc
     1621 gcgtgtgcga cgcgatggcg cagcacgtgc gcgaggtcgt cgcgcggggc cacgacgtcg
     1681 aggcgatctc ggcgggcggc gggttgtcga tcccgtaccg gcccggcgac gcggtcgtcg
     1741 acacggcgca ctacttcagc ctgtgggacg cggcgcgccg gcagctcgcg cgcgagctcg
     1801 ggcatccgct gcggctggag atcgagccgg gccgcttcct cgtcgccgag tcgggcgcga
     1861 tgctggccga ggtgcgtgcc gtcaaggaca tgggcgccaa ccacttcgtg ctctgcgacg
     1921 ccgggttcaa cgacctcgtg cgacccgcga tgtacggcgg ccaccatgcg atcagcgtcg
     1981 tcgcgcgcac gctgcacgag ggctcgcagg cgccccgtcc caccgtcgtc gccgggccgc
     2041 tgtgcgagtc gggcgacgtc ttcacgcagg acgccggcgg cgtcgtcgag ccgcgcccgc
     2101 tgccgcccgc ccagccgggc gacctgctgg tcttccacga tgccggggcg tatggcgcgt
     2161 cgatgagctc caactacaac tcccgtccgc tcgcggccga ggtgctggtc gacggcgacc
     2221 aggcacgccg catccggcga cggcagacgg tgcaggagct gatcgcgctg gagaactgag
     2281 gacgggctca ggcgccgccg gcgcgcagcc cgtccagcac gcgccgcacg gtcgcgaccg
     2341 cctcgcgccg g
//
LOCUS       DASVLN010000647         4200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_355804, whole genome shotgun sequence.
ACCESSION   DASVLN010000647 DASVLN010000000
VERSION     DASVLN010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355804"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VF453_15335"
     CDS             <1..1191
                     /locus_tag="VF453_15335"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX7689087.1"
                     /translation="GGGRAPRGGPGDDDADRGVIGSGFILSPDGVVLTNAHVVEGADE
                     VTVTLADKHEYKARIIGTDARTDVAVLKIAASNLPFVRIGDVDRLKVGEWVMAIGSPF
                     GLESTVTAGIVSGKHRETGDYLQLLQTDVAINPGNSGGPLINMRGEVVGINSQILSPS
                     GASAGISFSIPIDEAIRVADQLRASGHVVRGRIGVQIGPVSRPVAESIGLGKPQGALV
                     GSVQPGLPADAAGIEPGDVITKVQGQVIDQSSDLPRMIGMIKPGDKAHLEVWRLGRRI
                     DVTTNVVDFGADADKPDADEGQPPADKSNSAFGMRVSDLADVERARLHVKGGVRVEAA
                     EGAADRAGLQEGDIVLAVGDVRITSVKQFLATAAKVDKAKTVPVLVRRGDSATWLLIK
                     PAAK"
     gene            1336..3000
                     /gene="ettA"
                     /locus_tag="VF453_15340"
     CDS             1336..3000
                     /gene="ettA"
                     /locus_tag="VF453_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX7689088.1"
                     /translation="MAQYVFTMNRLGKTVPPKRQILKGISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGVDKDFEGEAQPMPGLNIGYLPQEPQLDPTQTVRQSVEEGIGGVLAAKK
                     RLDEVYAAYAEPDADFDALAAEQGQLEAIIAAAGSENTEHQLEIAADALRLPPWDAVI
                     GNLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVDWLEQFLSRFTGTVVAITHD
                     RYFLDNAAEWILELDRGQGIPYKGNYSTWLLQKEERLEQEQRSEDARMKAMKKELEWV
                     RQNPKGRQAKSKARIARFEELSDFDYQKRNETNEIFIPVADRLGTQVIEFDGVTKSFG
                     DRTLIDNLSFKVPAGAIVGIIGPNGAGKSTLFRMIAGKEQPDAGTVTIGSTVKMAFVD
                     QSREDLANDKTVWEDVSGGLDNLTVGKFVMPSRAYIGRFNFKGNDQQKLVGNLSGGER
                     GRLHLAKTLIQGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLDRIA
                     THILACEGDSQWVFFDGNYQEYEADKKKRLGEEGARPKRVRYKALK"
     gene            3247..3855
                     /locus_tag="VF453_15345"
     CDS             3247..3855
                     /locus_tag="VF453_15345"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7689089.1"
                     /translation="MRFRQLCKLACVASAVVASAAANATVVTFDSLANAGQYQFAGDG
                     ITYSESGLTFTSNLPSPESLLVWGAQSGYDADPHGGATLAQNYFGQYLTITKAGGGAF
                     TLNSIDIADVWNQGSGEDVNYTWVDAGGSHMSSVTLDRQPGLQTFDFNLSGVTSFALE
                     AGGSFQMDNVTYSTSSVPEPGSLALMTAGLLGLAGAVRRRRA"
     gene            complement(4063..>4200)
                     /gene="rpmG"
                     /locus_tag="VF453_15350"
     CDS             complement(4063..>4200)
                     /gene="rpmG"
                     /locus_tag="VF453_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX7689090.1"
                     /translation="LESTAGTGHFYTTSKNKKTTPEKLEFMKFDPKARKHVLYKEIKL
                     R"
BASE COUNT          703 a         1454 c         1427 g          616 t
ORIGIN      
        1 ggcggcgggc gcgcgccgcg cggcggcccc ggcgacgacg acgcggaccg cggcgtgatc
       61 ggctccggct tcatcctctc gcccgacggc gtcgtgctga ccaacgccca cgtcgtcgag
      121 ggcgccgacg aggtcaccgt cacgctggcc gacaagcacg agtacaaggc gcggatcatc
      181 ggcacggacg cccgcaccga cgtcgccgtg ctcaagatcg ccgcctcgaa cctgccgttc
      241 gtgcgcatcg gcgacgtcga ccgcctgaag gtcggcgagt gggtcatggc gatcggctcg
      301 cccttcggcc tggaaagcac ggtcaccgcc ggcatcgtca gcggcaagca ccgcgagacc
      361 ggcgactacc tgcagctgct ccagaccgac gtcgcgatca accccggcaa ctcgggcgga
      421 ccgctgatca acatgcgcgg cgaggtggtg gggatcaact cgcagatcct cagcccgtcc
      481 ggcgcgagcg cgggcatctc gttctcgatc ccgatcgacg aggcgatccg cgtcgccgac
      541 cagctgcgcg cgagcgggca cgtcgtgcgc gggcgcatcg gcgtgcagat cgggccggtc
      601 tcgcgaccgg tcgccgagtc catcggcctg ggcaagccgc agggcgcgct ggtcggcagc
      661 gttcagccgg gcctgcccgc ggacgccgcc ggcatcgagc cgggcgacgt catcacgaag
      721 gtgcagggcc aggtgatcga ccagtcctcc gacctgccgc gcatgatcgg catgatcaag
      781 ccgggcgaca aggcgcacct cgaggtctgg cgccttggcc gcaggatcga cgtcacgacc
      841 aacgtcgtcg acttcggcgc cgacgcggac aagcccgacg ccgacgaggg ccagccgccc
      901 gccgacaagt ccaactccgc cttcggcatg cgggtgagcg acctcgccga cgtggagcgc
      961 gcgcgcctgc acgtcaaggg cggcgtgcgc gtcgaggccg ccgagggcgc ggccgaccgc
     1021 gcgggcctcc aggagggcga catcgtcctc gcggtgggcg atgtgcggat cacctcggtc
     1081 aagcagttcc tcgcgaccgc ggcgaaggtg gacaaggcga agaccgtgcc ggtgctcgtg
     1141 cgccggggcg actcggcgac gtggctgttg atcaagcctg cggccaagta aaataacgcc
     1201 tttgcccgcg ggcccggtgg ctgatgcagt cgccgggccg cgccgcttcc cgcatttccc
     1261 gcgcgcccgg cgcccgccgg cgcggacgag tcccgtccgc cgcgcatccc cgcttcccat
     1321 cccgaaggtc gccagatggc acagtacgtt ttcacgatga accggctcgg caagaccgtg
     1381 cccccgaagc gccagatcct caagggcatc tcgctcagct tcttccccgg cgcgaagatc
     1441 ggcgtgctgg gcctgaacgg ctcgggcaag tcgacgctgc tgaagatcat ggcgggcgtc
     1501 gacaaggact tcgagggcga ggcccagccc atgcccggcc tgaacatcgg ctacctgccg
     1561 caggagccgc agctcgaccc gacgcagacc gtgcgccagt cggtcgagga aggcatcggc
     1621 ggcgtgctgg ccgccaagaa gcgcctggac gaggtctacg ccgcgtacgc cgagccggac
     1681 gccgacttcg acgcgctcgc ggcggagcag ggccagctcg aggcgatcat cgcggccgcg
     1741 ggctcggaga acaccgagca ccagctggag atcgccgccg atgcgctgcg cctgccgccc
     1801 tgggacgcgg tgatcggcaa cctctccggc ggcgagaagc gccgcgtcgc gctgtgccgg
     1861 ctgctgctgt ccaagcccga catgctgctg ctcgacgagc cgacgaacca cctggacgcc
     1921 gagtccgtgg actggctcga gcagttcctc tcccgcttca ccggcaccgt cgtcgcgatc
     1981 acccacgacc gctacttcct cgacaacgcc gccgagtgga tcctcgaact cgatcgcggc
     2041 cagggcatcc cgtacaaggg caactactcg acgtggctgc tgcagaagga agagcgcctc
     2101 gagcaggagc agcgttccga ggacgcccgg atgaaggcga tgaagaagga actggagtgg
     2161 gtccgccaga accccaaggg ccgccaggcc aagagcaagg cccgcatcgc gcgcttcgag
     2221 gagctgtcgg acttcgatta ccagaagcgc aacgagacca acgagatctt cattcccgtg
     2281 gcggatcgcc tgggcacgca ggtcatcgag ttcgacggcg tcacgaagag cttcggggac
     2341 cgcacgctga tcgacaacct gagcttcaag gtgcccgccg gcgcgatcgt cggcatcatc
     2401 ggcccgaacg gcgccggcaa gtcgacgctg ttccgcatga tcgcgggcaa ggagcagccg
     2461 gacgcgggca cggtgacgat cggctcgacg gtcaagatgg ccttcgtcga ccagagccgc
     2521 gaggacctgg ccaacgacaa gaccgtgtgg gaggacgtct cgggcggcct cgacaacctg
     2581 accgtgggca agttcgtgat gcccagccgc gcctacatcg gccgcttcaa cttcaagggc
     2641 aacgaccagc agaagctcgt cggcaacctg tccggcggcg agcgcggccg cctgcacctg
     2701 gccaagacgc tgatccaggg cggcaacgtg ctgctgctgg acgagccgtc caacgacctc
     2761 gacgtcgaga cgctgcgcgc cctcgaggac gcgctgctgg agttcgccgg ctcggtcatg
     2821 gtcatcagcc acgaccgctg gttcctcgac cgcatcgcca cccacatcct cgcgtgcgag
     2881 ggcgattcgc agtgggtttt cttcgacggc aactaccagg agtacgaggc cgacaagaag
     2941 aagcgcctcg gcgaggaagg ggcgcgtccg aagcgcgtgc gctacaaggc gctcaagtga
     3001 cgcctcgggc gtcccgctgc cggagaagcc gccctcgagg cggctttttc atggccgcat
     3061 gacaggccgc ggcacgccat cccggaaacg ggcgcctgcc cccccggggg gagggggcgt
     3121 gaacttcagc acggtatgcc gtggcacggg gtcacgttca ccggaattgg ggaggtacct
     3181 gctcgacagg agcaagtatg gtgggcgccg atcctatcca ccatacatcc cgagggagag
     3241 agaagcatga ggttccgaca gctctgcaag ctggcctgcg tggcgtccgc cgtggttgct
     3301 tcggcggccg cgaacgcgac cgtcgtcacg ttcgactcgc tggccaacgc cggccagtac
     3361 cagttcgccg gcgacggcat cacgtattcc gagagtggcc tgaccttcac ctcgaacctg
     3421 ccgtcgcccg agtcgctgct cgtgtggggc gcgcagtcgg gctacgacgc cgatccgcac
     3481 ggcggcgcga ccctggcgca gaactatttc ggccagtatc tgaccatcac gaaagccggc
     3541 ggcggcgcgt tcaccctgaa ctcgatcgac atcgccgacg tctggaatca gggctccggc
     3601 gaggacgtca actacacctg ggtcgacgcc ggcggctcgc acatgtcgag cgtcacgctg
     3661 gacagacagc ccgggctgca gaccttcgac ttcaaccttt ccggcgtgac ctcgtttgcg
     3721 ctcgaggcag gcggcagctt ccagatggat aacgtgacct acagcacttc cagcgtgccc
     3781 gagcccggtt cgctggcgct gatgacggcc ggcctgctgg gactcgccgg cgcggtgcgc
     3841 cgccgcaggg cctgagcgga agttccttcg ctgtccaagc cgcccacggg cggctttttc
     3901 catgctacgc gaccgatggc cggcgccgct cggactgtcg cgccgcttcg aaaagcgtgt
     3961 ccctcgagaa ttccatccga gcgacgcgca tgcaaaaagc cgccctgagg cggctttcgt
     4021 tcggtgtcgt cgacgcgcgc tgcggggcag cgcgccgggc gatcagcgca gcttgatttc
     4081 cttgtacagg acgtgcttgc gcgccttcgg gtcgaacttc atgaattcga gcttttccgg
     4141 cgtcgtcttc ttgttcttgc tggtggtgta gaagtggccc gttccggcag tggattcgag
//
LOCUS       DASVLN010000648         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_194631, whole genome shotgun sequence.
ACCESSION   DASVLN010000648 DASVLN010000000
VERSION     DASVLN010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194631"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..2265
                     /locus_tag="VF453_15355"
     CDS             67..2265
                     /locus_tag="VF453_15355"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7689091.1"
                     /translation="MPRSAVALRTFCAVLAGAAALAHAAPPAPPVAPVKPVTDTYFGT
                     TVTDTYRYMEDLSNPEVQQWAKGQADYARAVLDAIPGRAPLLKRIAELEASVAARIVG
                     VHVLPSGQVFYQKRAASDNQFKLYVRQGFDGAERLLVDPEAMAKAAGGTPQAINFYEP
                     SPNGRYVVYGVSQGGSEESLIHVVDSASGKELITPIPRTHYSDAWWMPDESGFFYFGQ
                     RELAKGAPETDKYKHQLAYFHRMKGRGADQVVLEAGKSDHLPIGEAEFPAVSPVLGTR
                     WAVAAPGNGVQNEIALYAAPLGRVLDPKLKWRRLFDRDADVTAFAIHGDDLYLLTHKN
                     ASRYKVVKTSMTHPDLEQAHVVVAPGREVVTGISAAKDGLYVQARDGTVGKLYRVAWS
                     ADAQPQPVALPTAGAVSIDDADPRLPGVLASVSSWTHDVVYYAPDRRTGAYVDTGLQP
                     PSPYGAPDSIESKEVMVKSWDNIEIPLSIVAPKGIKLDGSNPTVLYGYGAYGITDDPV
                     YIPRFLAWFELGGVRATCHVRGGGAYGEDWHLAGKQETKPNTWKDFIACAEYLVKEGY
                     TTPAKLGIHGGSAGGILVGRAMTARPDLFAAAVPEVGVLNALRSETSANGVPNIPEFG
                     TVADHRQFDSLLEMDAFHHVVDGTKYPAVLLMHGINDPRVPPWESFKMAARLQAATAS
                     GKPVLLRIDYAAGHGIGSTKTQRQEQYADMWSFMLWQFGDPRFQPPAAAH"
BASE COUNT          377 a          933 c          849 g          327 t
ORIGIN      
        1 atacggatgc gggtgccgcg agcgctgtcg gatactgcgc gcttcgcttc gtcgcaggag
       61 accccgatgc cacgttccgc cgtcgcgctg cgcacgttct gcgccgtgct cgccggcgcg
      121 gccgcgctcg cgcacgccgc gccgcccgcc ccgccggtcg ccccggtcaa gccggtgacc
      181 gatacctact tcggcaccac ggtgaccgac acctaccgct acatggagga cctctcgaac
      241 cccgaggtcc agcagtgggc gaagggccag gccgactacg cgcgcgcggt gctcgacgcg
      301 atccccggcc gcgcgccgct gctcaagcgc atcgccgagc tcgaggcgtc ggtggccgcg
      361 cgcatcgtcg gcgtgcacgt gctgccctcg ggccaggtct tctaccagaa gcgcgcggcg
      421 agcgacaacc agttcaagct ctacgtccgc cagggcttcg acggcgccga acgcctgctc
      481 gtcgaccccg aggcgatggc caaggccgcc ggcggcacgc cgcaggcgat caacttctac
      541 gagccctcgc cgaacggccg ctacgtcgtc tacggcgtgt cgcagggcgg gtcggaggag
      601 tcgctgatcc acgtcgtcga cagcgcgagc ggcaaggagc tgatcacgcc gatcccgcgc
      661 acgcactaca gcgacgcctg gtggatgccc gacgagagcg gcttcttcta cttcggccag
      721 cgcgagctcg ccaagggcgc gccggagacc gacaagtaca agcaccagct cgcctacttc
      781 caccgcatga agggccgcgg cgccgaccag gtcgtgctgg aggccggcaa gtccgaccac
      841 ctgccgatcg gcgaggcgga gttccccgcg gtctcgccgg tgctgggcac gcgctgggcc
      901 gtcgcggcac cgggcaacgg cgtgcagaac gagatcgccc tgtacgccgc gccgctcggg
      961 cgcgtgctcg atccgaagct caagtggcgc cgcctgttcg accgcgacgc cgacgtcacc
     1021 gccttcgcga tccacggcga cgacctgtac ctgctcacgc acaagaacgc gtcgcgctac
     1081 aaggtcgtca agacgagcat gacccatccc gacctcgaac aggcgcacgt ggtcgtcgcg
     1141 cccgggcgcg aggtcgtgac gggcatctcc gcggccaagg acggcctgta cgtgcaggcg
     1201 cgcgacggca ccgtcggcaa gctgtaccgc gtcgcgtgga gcgcggacgc gcagccgcag
     1261 ccggtggcgc tgccgacggc gggcgcggtg tccatcgacg acgccgaccc gcgcctgccg
     1321 ggcgtgctcg cgtcggtctc gtcgtggacg cacgacgtcg tctactacgc gccggaccgc
     1381 aggacgggcg cgtacgtcga caccggcctg cagccgccga gcccgtacgg cgcgcccgac
     1441 tcgatcgagt cgaaggaggt gatggtcaag agctgggaca acatcgagat cccgctgtcc
     1501 atcgtcgcgc ccaagggcat caagctcgat ggcagcaatc cgaccgtgct gtacggctac
     1561 ggcgcctacg gcatcaccga cgacccggtc tacatcccgc gcttcctcgc ctggttcgag
     1621 ctgggcggcg tgcgcgcgac ctgccacgtg cgcggcggcg gcgcgtacgg cgaggactgg
     1681 cacctcgcag gcaagcagga gaccaagccc aacacctgga aggacttcat cgcctgcgcc
     1741 gagtacctgg tcaaggaggg ctacaccacg cccgcgaagc tgggcatcca cggcggcagc
     1801 gcgggcggca tcctggtcgg ccgcgcgatg acggcgcgtc ccgacctgtt cgcggcggcg
     1861 gtgcccgagg tcggcgtgct caacgcgctg cgctcggaga cctccgccaa cggcgtgccc
     1921 aacatccccg agttcggcac ggtggccgac caccggcagt tcgacagcct gctggagatg
     1981 gacgccttcc accatgtcgt cgacggcacg aaatacccgg cggtgctgct gatgcacggg
     2041 atcaacgacc cgcgcgtgcc gccgtgggag tccttcaaga tggcggcgcg cctgcaggcc
     2101 gccaccgcat cgggcaagcc ggtgctcctg cgcatcgact acgccgccgg ccatggcatc
     2161 ggcagcacca agacgcagcg ccaggagcag tacgccgaca tgtggagctt catgctgtgg
     2221 cagttcggcg atccgcgctt ccagcctccg gccgcggccc actgaagcgg cggcgggcgc
     2281 gcggccgcga aggctgcggc ggggcttcgc cacccgcgtc tgcaggcgcg gccgagccgg
     2341 cacagaatgc gcgcttcgcg ccgctgcctg cggccacgtt cccagaccgg aaaggatcct
     2401 ccacgatgtc ccgttcccgc ctcacccgcg cggcccgcgc cgccgtcctc gccgccgcgg
     2461 cggccttcgc ctgcggcctc gccgtc
//
LOCUS       DASVLN010000649         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_303802, whole genome shotgun sequence.
ACCESSION   DASVLN010000649 DASVLN010000000
VERSION     DASVLN010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303802"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..641
                     /locus_tag="VF453_15360"
     CDS             192..641
                     /locus_tag="VF453_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX7689092.1"
                     /translation="MSTLKERITEDMKAAMRAKESDRLLAIRNLQAAIKQKEVDERIV
                     LDDAAVVAVVDKLVKQRKDSIDAFSKAGRTDLVDKESAELAVLSAYLPQRLDADAIAA
                     EVAAVVAELGASGPGDMGKVMAAVKAKLAGKAEMGQVSAAVKAALAK"
     gene            669..1016
                     /locus_tag="VF453_15365"
     CDS             669..1016
                     /locus_tag="VF453_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEX7689093.1"
                     /translation="METTLPLRQIDHDVFVAPQLEPAAMPELARLGFKSVVNNRPDFE
                     HGPDQPASADIAAAAKAAGLEYRHLPVAGGYQSPEEVAAFGQLVAELPRPLLAFCRSG
                     ARSTRLYQLAQGS"
     gene            1286..2179
                     /locus_tag="VF453_15370"
     CDS             1286..2179
                     /locus_tag="VF453_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689094.1"
                     /translation="MQVVLRNVLVTGAVVAAGGVAAVSQATNYSLWINGRNGGGAVAN
                     YADFTYWGPSSAAAGVNKKAVNWDGHSHVSDQNYLVRNALDCYCTGPNWCYVAAHSAG
                     DLMIGYALSMYGGSTRQVKNATPDASGACGNASGNPTQTGWNIKWVDIAAGAGGGSEL
                     ADVGDWTTSEPLVSDLKTATARAMYDHNNTRSHWFYMYAGAAGTLYSFVLPGQDDEAV
                     AYHSSGGVAGASGGAYCNPSDWFCNDLTLGTAANEGGSAKWSWHTVAFRDDGEKYNHY
                     TNGQWQGVVSLVRQDMANNAR"
BASE COUNT          384 a          840 c          805 g          279 t
ORIGIN      
        1 ctggccaagt accgcgcgat cccggccgcg gcggtcgcgc gcgccatggt cgccgcggcg
       61 cgcgcgcgac ggcccggcgc cacgatccac cactggcgcg agatgcaggc cgcggcgccg
      121 ggataatcgg ttgtctcgcg cggcgttcgc gcgcgccacg aacacgaaca ctcgaaagga
      181 acgtcgacga catgagcacg ctcaaggaac gcatcaccga agacatgaag gccgcgatgc
      241 gggccaagga atccgaccgc ctgctcgcga tccgcaacct gcaggccgcg atcaagcaga
      301 aggaggtcga cgagcgcatc gtgctcgacg acgccgcggt ggtcgcggtc gtcgacaagc
      361 tcgtgaagca gcgcaaggac tcgatcgacg ctttctcgaa ggccggccgc accgacctcg
      421 tcgacaagga atccgccgaa ctggccgtgc tgagcgccta cctgccgcag cgcctggatg
      481 ccgatgccat cgccgccgag gtggcggccg tcgtcgccga gctgggggcc tcgggcccgg
      541 gcgacatggg caaggtgatg gcggccgtga aggcgaagct cgccggcaag gccgagatgg
      601 gccaggtgtc ggcggcggtc aaggccgcgc tggccaagtg acacgcacgt gattcgaagg
      661 agacccgcat ggaaaccacc ctgcccctgc gccagatcga ccacgacgtc ttcgtcgccc
      721 cgcagctcga gccggccgcg atgccggagc tcgcgcggct cggcttcaag agcgtcgtga
      781 acaaccgccc ggacttcgag cacggccccg accagccggc ctccgccgac atcgccgccg
      841 ccgcgaaggc cgccggcctc gaataccgcc acctgccggt ggccggcggc taccagtcgc
      901 ccgaggaagt ggcggccttc ggccagctgg tggccgagct gccgcgtccc ctgctggcgt
      961 tctgccgctc gggcgcgcgc tcgacgcggc tgtaccagct cgcccagggc agctgagcgg
     1021 cgccgccgca cgcgcgccgt tcgccgggag gccggctgcg cgcataccca gtgccgtata
     1081 gcgggcaccg tgcgcatgac gcgcgccggc accgcgtgaa ccgggtgacg cgacttcgat
     1141 ccacgtcacc cggaaccagc atccatgcgg gctggcgggc catcgcccgg cgcgggaaaa
     1201 cgtgcattgc cgctcccgcg cgatgtcgac acattgcgca cacacgtctt ttcgtccgtc
     1261 cccgacacgc gagaaaggtg cccgcatgca agtcgtcctc cgcaacgtcc tggtcaccgg
     1321 cgccgtcgtg gccgcaggcg gcgtcgccgc ggtctcgcag gcgacgaact actcgctctg
     1381 gatcaacggc cgcaacggcg ggggcgcggt cgccaactac gccgacttca cctactgggg
     1441 cccgagctcg gcggccgccg gcgtcaacaa gaaggcggtc aactgggacg gccacagcca
     1501 cgtctccgac cagaactacc tcgtgcgcaa tgcgctcgac tgctactgca cgggcccgaa
     1561 ctggtgctac gtcgcggcgc acagcgcggg cgacctgatg atcggctacg cgttgtcgat
     1621 gtatggcgga tcgacgcgcc aggtcaagaa cgcgacgccc gacgcgagcg gtgcctgcgg
     1681 caacgccagc ggcaacccga cgcagaccgg gtggaacatc aagtgggtcg acatcgcggc
     1741 cggcgcgggc ggaggctcgg agctcgccga cgtcggcgac tggacgacct ccgagccgct
     1801 ggtctccgac ctgaagaccg cgaccgcgcg cgcgatgtac gaccacaaca acacgcgcag
     1861 ccactggttc tacatgtacg ccggcgccgc ggggacgctg tactccttcg tgctgccggg
     1921 ccaggacgac gaggcggtcg cgtaccacag ctcgggcggc gtcgcgggcg cctcgggcgg
     1981 cgcgtactgc aaccccagcg actggttctg caacgacctc acgctgggca cggccgccaa
     2041 cgagggcggc tcggcgaagt ggagctggca cacggtcgcc ttccgcgacg acggcgagaa
     2101 gtacaaccac tacaccaacg gccagtggca gggcgtcgtg agcctggtgc ggcaggacat
     2161 ggcgaacaac gccagatgac gagcccccac gcccgcggcc gcctgctgcc ctccgcgggg
     2221 gcgttcgcga gcggcccggc aaggccggat ccgccgctcc ccggcgcctc gcgtgaggcg
     2281 aagcgtcggc cgcgccgaag gctggcgg
//
LOCUS       DASVLN010000650         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_408937, whole genome shotgun sequence.
ACCESSION   DASVLN010000650 DASVLN010000000
VERSION     DASVLN010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408937"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VF453_15375"
     CDS             complement(<1..537)
                     /locus_tag="VF453_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HEX7689095.1"
                     /translation="MAVTTVAQLASELNRPVATLLDQLQSAGVAKKAAEDALSDADKE
                     RLLDHLRTMHGTTGGERKKITLTRKSTSEIKQADASGKARTIQVEVRKKRVFVKRDDE
                     TTEEATEAAAPAVPDEAELRRREEEARAQAEAIRQQEEELAEKRRQREAEERARAEAE
                     ERERQAAAERAAAEKAAAE"
     gene            complement(605..>1790)
                     /gene="nusA"
                     /locus_tag="VF453_15380"
     CDS             complement(605..>1790)
                     /gene="nusA"
                     /locus_tag="VF453_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7689096.1"
                     /translation="ESVPIGRIGAQAAKQVILQKIRDAEREQLLNDFLSRGERIFVGT
                     VKRLDKGDIIVESGRVEGRLKRGEMIPKENLRSGDRVRAFIAGVDPTARGPQIMLSRS
                     APGFMVELFAQEVPEIEQGLLEIKSCARDPGSRAKIAVLSHDKRVDPIGTCVGVRGSR
                     VTAVTNELAGERVDIVLWSEDPAQFVIGALAPANVVSIVVDEEKHAMDVVVDEENLAI
                     AIGRGGQNVRLASDLTGWRINIMTAEESQAKQAQELGGVRQLFVEKLDVDEEVADILI
                     EEGFTSLEQVAYVPLQEMLEIEGFDEDTVNELRTRAKDALLTMEIAREERVDEVSQDL
                     RDLEGLTPELIGRLADGGVHSRDDLADLAVDELVEMTGMDEAAAKALIMKAREHWFNG
                     AA"
BASE COUNT          206 a          649 c          592 g          343 t
ORIGIN      
        1 ctccgccgca gccttctcgg cagccgccct ttccgcggcg gcctggcgct cgcgctcctc
       61 ggcctcggcg cgtgcgcgct cctcggcctc gcgctggcgg cgcttctcgg cgagttcctc
      121 ttcctgctgg cggatcgcct cggcctgcgc gcgcgcctct tcctcgcggc ggcgcagctc
      181 ggcttcgtcg ggcacggccg gggcggcggc ctcggtcgcc tcctcggtcg tctcgtcgtc
      241 gcgcttgacg aacacgcgct tcttgcgcac ctccacctgg atcgtgcgcg ccttgccgct
      301 ggcgtcggcc tgcttgatct cgctggtcga cttgcgcgtc agcgtgatct tcttgcgctc
      361 gccgccggtc gtgccgtgca tggtgcgcag gtggtcgagc agccgctcct tgtctgcgtc
      421 gctcagcgcg tcctcggccg ccttcttggc gacgccggcg gactgcagct gatcgagcag
      481 cgttgcaacg gggcgattca gctccgatgc aagctgtgcg acggtggtca ctgccatttc
      541 tgggattcct tgctcgttga tccgggcgtt cggtgtatct gggttgcgtc gttcggctgg
      601 aggcttacgc ggcgccgttg aaccagtgct cgcgggcctt catgatgagg gccttcgcgg
      661 cagcctcgtc catgccggtc atctccacca gctcgtcgac ggccaggtcg gcgaggtcgt
      721 cgcgcgagtg cacgccgccg tcggccagcc gaccgatcag ctccggcgtc aggccttcga
      781 ggtcgcgcag atcctgcgac acctcgtcga cgcgttcctc gcgtgcgatc tccatcgtga
      841 gcagcgcgtc cttggcgcgg gtgcgcagct cgttgacggt gtcctcgtcg aagccctcga
      901 tctccagcat ctcctgcagg ggcacgtagg cgacctgctc gaggctggtg aagccttcct
      961 cgatcaggat gtcggcgact tcctcgtcga cgtccagctt ctcgacgaac agctggcgca
     1021 cgccgcccag ctcctgggcc tgcttcgcct gcgattcctc ggccgtcatg atgttgatcc
     1081 gccagcccgt caggtccgac gcgaggcgca cgttctggcc gccgcggccg atcgcgatcg
     1141 cgaggttctc ctcgtcgacc acgacgtcca tcgcgtgctt ctcctcgtcc acgacgatgg
     1201 agaccacgtt ggccggcgcg agcgccccga tcacgaactg ggccggatcc tcgctccaca
     1261 gcacgatgtc cacgcgctcg cccgcgagct cgttggtcac cgcggtgacg cgcgagccgc
     1321 gcaccccgac gcaggtgccg atcgggtcga cccgcttgtc gtgcgagagg acggcgatct
     1381 tggcgcgcga gccggggtcg cgggcgcagc tcttgatctc gagcaggccc tgctcgatct
     1441 cggggacttc ctgggcgaac agctcgacca tgaagcccgg agcgctgcgc gagagcatga
     1501 tttggggacc acgcgcggtc ggatcaaccc cggcgatgaa cgcacgcacg cggtcgcccg
     1561 agcgcaggtt ctccttcggg atcatctcgc cgcgcttcag gcggccctcg acgcgacccg
     1621 actcgacgat gatgtcgccc ttgtccagcc gcttgaccgt gccgacgaag atgcgctcgc
     1681 cgcgcgacag gaagtcgttg agcagctgct cgcgctcggc gtcgcggatc ttctgcagga
     1741 tgacctgctt ggccgcctgc gcgccgatgc ggccgatcgg caccgactcg
//
LOCUS       DASVLN010000651         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_33287, whole genome shotgun sequence.
ACCESSION   DASVLN010000651 DASVLN010000000
VERSION     DASVLN010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33287"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..467)
                     /locus_tag="VF453_15385"
     CDS             complement(114..467)
                     /locus_tag="VF453_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689097.1"
                     /translation="MAATDAPPIEVYVTVDVQGKPHVTCSPYTMDVSASTTLQFQLMS
                     SGWAFPSTNAVYLNTPPDANFPDPSVTVSSTLVTWTDAFVSAGDFGYSVCVQKSTGGY
                     ASTNDPVIHNNDSTH"
     gene            complement(476..835)
                     /locus_tag="VF453_15390"
     CDS             complement(476..835)
                     /locus_tag="VF453_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689098.1"
                     /translation="MTSTTETVVQISVNVTVDVRGNPHVTCTPYDASVSCPSTLQFNL
                     VTPGWVFPSTNAAYLNTPPDANFPAPAVTVSPTQATWQDVFACSGDIAYTVAVQRSTG
                     GPVYTQDPVIHNNDSAC"
     gene            complement(832..>2358)
                     /locus_tag="VF453_15395"
     CDS             complement(832..>2358)
                     /locus_tag="VF453_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7689099.1"
                     /translation="ALAAGVGVALWQAREASRQREQAEGLVEFMITDLRKKLEPVGRL
                     DVLDSVGDKALAYYAAQDLDRLDADSLGRRNRALHLIGDLAQQRGRLDEAERDFRQAA
                     DSTAALLRRHPGDGQRIFDHAQSEYWVGYTQWTRGRFTDAEASFRRYLALADRLRAIA
                     PDNLDWRLEQVYAATNVATALIDLGRPAEALATIEPAERNMADIAAARPDEAFDHATI
                     IGWLMRAQESLGRYDAAIATAQRKMAAARRAPNADTNASVRQVLGTALVDIGRLQMDL
                     GQRDAALATLRRGVADAQALAARDPSNLDWLFDAVRTQQQLEDLLLAMGDVDGARALD
                     RRVADESTKLLSTPRGKAGWRIALLGHDRVLKARLARDETERTAVVAALRDYLSDVRR
                     YELAGSELTPGERELVTRAGLALGDLLQHEGKPGEAAASWREAFDRNAPAAARGSPPA
                     MTLQGLLCLRLGRTQEARAWADRVLATSYRHPVFADLEDQLGPARMAGETPGPLQGNP
                     "
BASE COUNT          298 a          798 c          893 g          369 t
ORIGIN      
        1 tcacgcccgc ggcgctgcgg cgcgagctgc tggcgcgggt ggacgcgatc gcggcgctct
       61 accggcggcc gccgcagggc gccggagcgc gcggacgatg agccgccgga gcgtcagtgg
      121 gtgctgtcgt tgttgtggat gaccggatcg ttggtgctcg cgtagccccc ggtcgatttc
      181 tgcacgcaca ccgagtagcc gaagtcgccg gcgctcacga acgcgtccgt ccaggtcacc
      241 agggtggacg agacggtcac ggacgggtcg gggaagttgg cgtcgggcgg cgtgttgagg
      301 tagacggcgt tcgtggacgg aaaggcccag cccgacgaca tgagctggaa ctgcagcgtc
      361 gtggacgcgg acacgtccat cgtgtacggc gagcaggtga cgtgaggctt gccctggacg
      421 tcgacggtca cgtagacctc gatcgggggc gcatcggtgg cggccatgcg atgcctcagc
      481 aggcgctgtc gttgttgtgg atcaccgggt cctgcgtgta gaccgggccg ccggtcgagc
      541 gctgcaccgc gaccgtgtag gcgatgtcgc ccgagcacgc gaagacgtcc tgccaagtcg
      601 cctgcgtcgg gctcaccgtc acggccggtg ccgggaagtt ggcgtcgggc ggggtgttga
      661 ggtaggcggc gttcgtggac gggaagaccc agcccggcgt cacgagattg aattgcagcg
      721 tgctcgggca gctgacgctg gcgtcgtagg gcgtgcacgt gacatgggga ttgccgcgaa
      781 cgtccacggt cacgttgacg ctgatctgga cgacggtttc ggtggtgctg gtcatgggtt
      841 cccttgcaat gggcccggcg tctcccccgc catccgcgcc ggcccgagtt ggtcttcgag
      901 atcggcaaag acggggtgcc gataagaggt cgcaagcacc ctatcggccc aggcgcgcgc
      961 ttcttgagtc cggcccaggc gcaggcagag cagtccttgc agcgtcatcg cgggcgggct
     1021 cccgcgcgcc gcggcgggcg cgttgcggtc gaaggcctcg cgccacgagg cggccgcttc
     1081 gccaggcttg ccctcgtgct gcaggagatc cccgagcgcc aggcccgcgc gcgtgaccag
     1141 ctcgcgttcg cccggcgtca gctcgctgcc ggcgagctcg tagcgtcgca cgtcggagag
     1201 gtagtcccgc agcgcggcga cgacggccgt gcgctccgtc tcgtcgcgcg cgagccgggc
     1261 cttcagcaca cggtcgtggc ccagcagcgc gatgcgccag cccgccttgc cccgcggcgt
     1321 cgacagcagc ttcgtggatt cgtccgcgac acgccggtcc agggcgcggg cgccgtcgac
     1381 gtcgcccatc gccagcagga ggtcctcgag ctgctgctgc gtccgcaccg cgtcgaacag
     1441 ccagtcgagg ttgctcgggt cgcgcgccgc gagcgcctgg gcatccgcga cgccccggcg
     1501 cagggtcgcg agcgccgcgt cgcgttgccc caggtccatc tgcagccggc cgatgtcgac
     1561 gagcgccgtc ccgagcacct ggcgcacgct tgcgttcgtg tccgcgttcg gcgcccgccg
     1621 cgcggccgcc atcttgcgct gggccgtcgc gatcgcggcg tcgtagcgac cgaggctttc
     1681 ctgcgcacgc atgagccagc cgatgatggt ggcatggtcg aaggcctcgt cggggcgcgc
     1741 agcggcgatg tcggccatgt tgcgttcggc gggctcgatg gtggccagcg cctccgccgg
     1801 gcggccgagg tcgatcagcg cggtcgccac gttggtcgcg gcgtagacct gctcgagccg
     1861 ccagtcgagg ttgtccggcg cgatcgcgcg cagcctgtcg gccagcgcca ggtaacgccg
     1921 gaacgaggct tcggcgtcgg tgaagcggcc gcgcgtccat tgcgtatacc cgacccagta
     1981 ttcgctctgc gcgtggtcga agatgcgctg cccgtcgccg ggatggcgcc tgagcagcgc
     2041 cgcggtggag tcggccgcct ggcggaagtc gcgttcggcc tcgtcgaggc ggccgcgctg
     2101 ctgtgccagg tcgccgatca ggtggagcgc gcggttgcgg cgaccgaggg agtcggcatc
     2161 caggcggtcg aggtcctgcg cggcgtagta ggcgagggcc ttgtcgccga cgctgtcaag
     2221 cacgtcgagc cggcccacgg gctcgagctt cttgcgcagg tcggtgatca tgaattccac
     2281 caggccttcc gcctgctcgc gctggcggct cgcctcgcgc gcctgccaca gcgcgacgcc
     2341 cacgcccgcg gccagcgc
//
LOCUS       DASVLN010000652         4963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_409037, whole genome shotgun sequence.
ACCESSION   DASVLN010000652 DASVLN010000000
VERSION     DASVLN010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409037"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1309
                     /locus_tag="VF453_15400"
     CDS             269..1309
                     /locus_tag="VF453_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7689100.1"
                     /translation="MALMGFLKKQFIDVIEWTEPADGILAWRYPMQDHEIQNGAQLTV
                     REAQAALFVNEGKAADAFGPGLYTLNTRTLPLLTNLKNWDKLFASPFKSDVYFFSTRL
                     QTDQRWGTPQPVTLRDKDFGAIRVRAFGNYAWRVADPKLFQQQVSGTREVYRVEEIDG
                     QLRGLMLQHLSDAVAQSGIPFLDLAANQIEFATQIRNAVAPAFASLGIALDMVTVQNL
                     SLPDELQKILDQKIGMGMVGQDMGKFMQYQTAQAIPEFAKNAGQGGIAGDAMGLGAGV
                     ALGQVLAGNLSQGLNAGNPANNPASSVAATQAPSTQDVMGLIEKLGELKAKGILTDEE
                     FAAKKAELLKKL"
     gene            1326..2921
                     /locus_tag="VF453_15405"
     CDS             1326..2921
                     /locus_tag="VF453_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEX7689101.1"
                     /translation="MSTPPPASQRSWRAACPNCGAPVEFRSPASASAVCSFCRSTLVR
                     EGEALRKIGQSAELFDDFTPLQLGASGRYAGEGFVLVGRLQFGTEDGPWNEWHALFDN
                     GRDGWLSEDNGRHVFAFDLPVPAGAPDPAQLQPGSRAFVDGRAWNVGSRVQARLLAAE
                     GELHEPPPADRPFTIVDLRNESGEVATLDASLAPRLQWSVGRPVQLSELKMQGLREAS
                     EKTLQSQAPACPSCGAPLAITLSTSQSVVCGSCKSVVDLSKGIGPDMAHYAQENRYEP
                     EIPIGATGTLPTTRGATPLPWTVVGYMERIELASGGDGEAEAWREYLLYNRNEGFAFL
                     VDTNEDWSLVRTLTGAPRVAGDRATWAGRDYTRRWTYDSTVTYVLGEFYWRVEAHQRT
                     RHQDFEASASGYRALLSLESTAAESLWSAGETLPAELVAQAFRLAAPQAARLERAGPP
                     AGGLDIGGFRVSWLMLIVLLVVVILLLAMCSHDSCAGVRKAYGDASPEYQQCRIRQAA
                     GTGYYAGTGGAWGGYSSGGGGHK"
     gene            2965..3189
                     /locus_tag="VF453_15410"
     CDS             2965..3189
                     /locus_tag="VF453_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX7689102.1"
                     /translation="MNVPEWLHPEVLGGTILYAVIGVVVLWLAFVVIDKITPYHLWDQ
                     IVREKNVALAIVVGAMFIALGMIIAAAIHG"
     gene            3189..4778
                     /locus_tag="VF453_15415"
     CDS             3189..4778
                     /locus_tag="VF453_15415"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX7689103.1"
                     /translation="MAARPRSGDARAPVDARVAVGPGEVALLASVFVVAACGLVYELC
                     AGALASYLLGDSVLQFSTVIGTYLFAMGLGSWASRHVREPLAPTFVRVELIVGVVGGL
                     MPAALFMAHATLPAAALASFRVLLYALVSIVGIGVGLEIPIVLRLLDRHLRGRDALAT
                     LVSQVLTFDYLGSLVVSLAFPLLLVPHLGIVRSGVFFGLLNTLVAAWSLWLFRGEVPR
                     WRARGVECVVVFAGLVAAMAAGDALTTWSEDRFYGPGIVLQEQTPYQRIVVTSQSGAI
                     RLFLNGNLQFNSKDEYRYHEALVHPAMAGFGAPRRVLVLGGGDGLAIREILKYPSVRQ
                     VTQVELDPEMTRLFTTQPMLTALNADALHDPKVRIVNADAFTWLEQHDDAYDVIVVDF
                     PDPSNFALGKLYSTSFYELVDQHLSASGYAVVQTTSPLVARRSFWTVAATLEAAGLRT
                     TPYHVHVPSFGEWGFIIASRRPWVAPRALPPGLRFLTLPGLQALQDFPPDMARVPAEP
                     NRLSNQTLVRTFEQEWGKVHD"
     gene            4771..>4963
                     /locus_tag="VF453_15420"
     CDS             4771..>4963
                     /locus_tag="VF453_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689104.1"
                     /translation="MTEGDGWSRRGFLGATLAAPAIVTGCAKPPADWRRHVEGRWVGD
                     DVALGHRVRDGAKDAAAHPP"
BASE COUNT          677 a         1816 c         1750 g          720 t
ORIGIN      
        1 gtccgcggcg cagcggtgca cgtgcccgac tcgtgccgac ctgccggcgt cgcagccgca
       61 gtccgcggcg gcggggcggg cgctcctgct ggcgtgcgcc gattccacgg gggtctccct
      121 tcgcgccggc accaggggcg catccgcgcg agtgtggcgt cccgccgcgc ccgcgtacag
      181 ggcgccgacc cgctcaactt agggaggacg cccgccgccg agcgactatc atccgccgac
      241 cgagacagcc cgccacgtgg aggcagccat ggccctgatg ggattcctca agaagcagtt
      301 catcgacgtc atcgagtgga cggagccggc cgacggcatc ctcgcctggc gctacccgat
      361 gcaggatcac gagatccaga acggcgcgca gctcacggta cgcgaggcgc aggccgccct
      421 gttcgtcaac gagggcaagg ccgccgacgc gttcgggccg ggcctgtaca cgctcaacac
      481 gcgcacgctg cccctgctga ccaacctgaa gaactgggac aagctcttcg cctcgccgtt
      541 caagagcgac gtctacttct tcagcacgcg cctgcagacg gaccagcgct ggggcacgcc
      601 gcagcccgtc acgctgcgcg acaaggattt cggcgccatc cgcgtgcgcg cgttcggcaa
      661 ctacgcctgg cgcgtcgccg acccgaagct gttccagcag caggtctcgg gcacgcgcga
      721 ggtctaccgg gtcgaggaga tcgacggcca gctgcgcggg ctgatgctgc agcacctgtc
      781 cgacgcggtc gcgcagagcg gcatcccctt cctcgacctc gccgccaacc agatcgagtt
      841 cgcgacccag atccgcaatg ccgtcgcccc ggccttcgcg agcctcggca tcgcgctgga
      901 catggtgacg gtgcagaacc tgtcgctgcc cgacgagctg cagaagatcc tcgaccagaa
      961 gatcggcatg ggcatggtcg gccaggacat gggcaagttc atgcagtacc agacggcgca
     1021 ggcgatcccc gagttcgcga agaacgccgg ccagggcggc atcgcgggcg acgcgatggg
     1081 cctgggcgcg ggcgtggcgc tggggcaggt gctcgcgggc aacctgtcgc aggggctcaa
     1141 tgccggcaac cccgccaaca acccggcctc gtccgtcgcc gccacccagg cgccgtccac
     1201 gcaggacgtg atgggcttga tcgagaagct gggcgagctc aaggccaagg gcatcctgac
     1261 cgacgaggaa ttcgccgcca agaaggccga gctgctgaag aagctctgac gccgcgccgc
     1321 gcgacgtgag cactccgcca cccgccagcc agcgcagctg gcgcgccgcc tgcccgaact
     1381 gcggcgcgcc ggtcgagttc cgctcgcccg cctcggccag cgcggtctgc agcttctgcc
     1441 gcagcacgct ggtgcgcgag ggcgaggcgc tgcgcaagat cggccagagc gccgagctct
     1501 tcgacgactt caccccgctg cagctcggcg cctccgggcg ctacgccggc gagggcttcg
     1561 tgctggtcgg ccgcctgcag ttcggcaccg aggacggtcc gtggaacgag tggcacgccc
     1621 tgttcgacaa cggccgcgac ggctggctgt cggaggacaa cggccgccac gtcttcgcgt
     1681 tcgacctgcc ggtgcccgcc ggcgcccccg atccggcgca gttgcagccc ggctccaggg
     1741 ccttcgtcga cggccgcgcc tggaacgtcg gctcgcgggt gcaggcgcgc ctgctggccg
     1801 ccgagggcga actgcacgag ccgccgccgg ccgaccggcc gttcaccatc gtcgacctgc
     1861 gcaacgagtc gggcgaggtc gccacgctgg atgcgagcct cgcgccgcgg ctgcagtggt
     1921 cggtcgggcg cccggtgcag ctgtccgagc tgaagatgca ggggctgcgc gaggcctcgg
     1981 agaagacgct ccagtcgcag gcgcccgcct gccccagctg cggcgcgccg ctcgcgatca
     2041 cgctgtcgac ctcgcagtcg gtcgtctgcg gcagctgcaa gtcggtcgtc gacctgtcca
     2101 agggcatcgg cccggacatg gcgcactacg cgcaggagaa ccgctacgag cccgagatcc
     2161 cgatcggcgc caccggcacg ctgccgacca cgcggggcgc gacgccgctg ccctggacgg
     2221 tcgtcggcta catggagcgg atcgagctcg cgtccggtgg cgacggggaa gccgaggcct
     2281 ggcgcgagta cctgctctac aaccgcaacg agggcttcgc gttcctggtc gacacgaacg
     2341 aggactggag cctcgtgcgc acgctcacgg gcgcgccgcg cgtcgcgggc gatcgcgcga
     2401 cctgggccgg ccgcgactac acgcgccgct ggacctacga ctcgaccgtc acctacgtcc
     2461 tcggcgagtt ctactggcgc gtcgaggccc accagcgcac gcgccaccag gacttcgagg
     2521 cctcggccag cggctaccgc gcgctgctga gcctggagag caccgccgcc gagagcctct
     2581 ggtcggccgg cgagacgctg cctgccgagc tggtcgcgca ggccttccgc ctggcagccc
     2641 cgcaggccgc gcggctggag cgcgccggcc cgcccgcggg cgggctggac atcggcgggt
     2701 tccgcgtctc gtggctgatg ctgatcgtgc tgctggtcgt cgtgatcctg ctgctcgcga
     2761 tgtgcagcca cgacagttgc gccggcgtac gcaaggccta cggcgacgcg agccccgagt
     2821 accagcaatg ccgcatccgc caggccgcgg gcaccggcta ctacgccggc accggcggcg
     2881 cctggggcgg ctattcgtcc ggcggcggcg gccacaagtg accgcacccg ccctttcccg
     2941 catccaccga aaaggagccg acgcatgaac gtaccagagt ggctgcaccc cgaggtgctg
     3001 ggcggcacga tcctctacgc cgtgatcggc gtggtcgtgc tgtggctcgc cttcgtggtc
     3061 atcgacaaga tcacgccgta ccacctgtgg gaccagatcg tccgcgagaa gaacgtcgcg
     3121 ctcgcgatcg tcgtcggggc gatgttcatc gcgctgggga tgatcatcgc cgcggcgatc
     3181 cacggttgat ggcggcgcgc ccgcgctccg gcgacgcgcg cgcgcccgtc gacgcacgcg
     3241 tcgccgtggg cccgggcgaa gtcgccttgc tcgcgtcggt cttcgtcgtc gccgcctgcg
     3301 gcctggtcta cgagctgtgc gccggcgcgc tcgcgagcta cctgctcggc gactcggtgc
     3361 tgcagttctc gaccgtcatc ggcacctacc tgttcgcgat gggcctgggc tcgtgggcct
     3421 cgcgccacgt gcgcgagccg ctcgcgccga ccttcgtgcg ggtggagctg atcgtcggcg
     3481 tcgtcggcgg cctgatgccg gcggccctgt tcatggcgca cgcgacgctg cccgcggcgg
     3541 cgctcgcgtc cttccgcgtg ctgctgtacg cgctcgtgag catcgtgggc atcggcgtgg
     3601 gactcgagat cccgatcgtg ctgcgcctgc tcgaccgcca cctgcgcggc cgcgacgcgc
     3661 tcgcgacgct ggtgtcgcag gtgctcacct tcgactacct cggctcgctg gtcgtctcgc
     3721 tcgcgttccc gctgctgctg gtccctcacc tcggtatcgt gcgctcgggc gtgttcttcg
     3781 gcctgctgaa cacgctggtg gcggcgtggt cgctctggct gttccgcggc gaggtgccgc
     3841 gctggcgcgc gcgcggcgtc gagtgcgtcg tcgtcttcgc gggcctggtc gccgcgatgg
     3901 ccgcgggcga cgcgctgacg acgtggtcgg aggatcggtt ctacgggccc ggcatcgtgc
     3961 tgcaggagca gacgccctac cagcgcatcg tcgtcacctc gcagtcgggc gcgatccggc
     4021 tgttcctcaa cggcaacctg cagttcaact cgaaggacga gtaccgctac cacgaggcgc
     4081 tcgtgcaccc ggcgatggcc ggcttcggcg cgccgcggcg cgtgctggtg ctggggggcg
     4141 gcgacgggct ggcgatccgc gagatcctga agtacccgtc ggtgcggcag gtcacgcagg
     4201 tcgagctcga ccccgagatg acgcgcctgt tcacgacgca gcccatgctg acggcgctca
     4261 acgcggacgc gctgcacgat cccaaggtgc gcatcgtcaa cgcggacgcc ttcacctggc
     4321 tcgagcagca cgacgacgcc tatgacgtga tcgtcgtcga cttccccgac ccgagcaact
     4381 tcgcgctcgg caagctctac tccaccagct tctacgagct cgtcgaccag cacctgtcgg
     4441 ccagcggcta cgcggtggtg cagacgactt ctccgctggt cgcgcggcgc agcttctgga
     4501 ccgtggccgc gacgctggag gccgccggcc tgcgcacgac gccgtaccac gtccacgtgc
     4561 cgagcttcgg cgaatggggc ttcatcatcg cgagccgccg gccgtgggtc gcgccgcgcg
     4621 ccctgccgcc gggcctgcgc ttcctcacgc tgccgggcct gcaggcgctg caggacttcc
     4681 cgcccgacat ggcgcgcgtg ccggccgagc ccaaccgcct gtccaaccag acgctggtgc
     4741 gcaccttcga gcaagagtgg ggcaaggtgc atgactgaag gcgacggctg gtcgcgccgc
     4801 ggcttcctgg gcgcgacgct ggccgcgccg gccatcgtga ccgggtgcgc gaagccgccc
     4861 gcggactggc ggcggcatgt ggagggccgc tgggtcgggg acgacgtggc gctcggccat
     4921 cgcgtgcgcg acggcgcgaa ggatgccgcc gcgcacccgc ccg
//
LOCUS       DASVLN010000653         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_519208, whole genome shotgun sequence.
ACCESSION   DASVLN010000653 DASVLN010000000
VERSION     DASVLN010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519208"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VF453_15425"
     CDS             complement(<1..1054)
                     /locus_tag="VF453_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX7689105.1"
                     /translation="MKTIRLTLAQALVRHLAALRVELDDGRVVPYFGGAFAIFGHGNV
                     AGLGEALHAERDRLPTWRAHNEQAMAHAAIAYAKANLRRRAMAVTTSIGPGATNLVTA
                     AALAHVNRLPVLLLPGDAFASRAPDPVLQQLEDFAHGDLSVNDCFRPVTRYFDRITRP
                     AQVLVALPQAIRAMTDPATCGPACLALPQDVQAEAFYCPEDFLAPPVLRIRRPAPDAM
                     ELAAAIETLRAAERPLIVAGGGALYSEAGAALAAFAQAHGIPVAETQAGKGSLAWDHP
                     LNVGAIGVTGSPAANACARGADLVVAVGSRLQDFTTGSHALFPQARLLGLNVQPADAH
                     KWHGQALVCDARVGLQA"
     gene            complement(1070..>1828)
                     /locus_tag="VF453_15430"
     CDS             complement(1070..>1828)
                     /locus_tag="VF453_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEX7689106.1"
                     /translation="GRVGVLIDDSLGEDALHAATGRGWWVGRPIELPGSRPLRFEGSG
                     SLGSQLVAWPREQVVKCLVHYHPDEDVDLRLEQEARLREAWAATRASGNELLLEVIPP
                     GMTPATPAADAAVLRAVTRLYTIGLKPEWWKLAPMQAPSWTRLAALVAARDPQCRGAV
                     ILGLNQPLDDLARGFAAATHPLVRGFMVGRSLWAGPASAWLAAPPGTAADEAFVAAVA
                     ANFEQLVDAWRATRPQGGTVPQPVAVSAAGVDAA"
BASE COUNT          224 a          666 c          721 g          217 t
ORIGIN      
        1 gtgcctgcag gccgacgcgc gcgtcgcaga ccagcgcctg gccgtgccac ttgtgcgcat
       61 cggcgggctg cacgttcagg cccagcagcc gcgcctgcgg gaacagcgcg tgcgagccgg
      121 tggtgaagtc ctgcaggcgc gagccgaccg ccaccacgag atcggccccg cgcgcgcagg
      181 cgttcgccgc gggcgagccc gtgacgccga tcgcgccgac gttgagcgga tggtcccacg
      241 cgaggctgcc cttgccggcc tgggtctcgg cgaccgggat gccgtgcgcc tgcgcgaacg
      301 cggcgagcgc ggcgccggcc tccgaataca gcgcgccgcc gccggccacg atcagcgggc
      361 gctcggcggc gcgcagggtc tcgatcgcgg ccgcgagttc catcgcgtcg ggcgccggcc
      421 ggcggatgcg cagcaccggc ggcgcgagga agtcctccgg gcagtagaag gcttcggcct
      481 gcacgtcctg cggcagcgcg aggcaggccg ggccgcaggt cgccgggtcg gtcatcgcgc
      541 ggatcgcctg cggcagcgcc acgaggacct gcgcgggccg cgtgatgcgg tcgaagtagc
      601 gcgtcaccgg ccggaagcag tcgttgacgc tcaggtcgcc gtgcgcgaag tcctcgagct
      661 gctgcagcac cgggtcgggg gcgcgcgagg cgaaggcgtc gcccggcagc agcagcacgg
      721 gcagccggtt cacgtgcgcg agcgccgcgg ccgtcaccag gttggtggcg cccggaccga
      781 tgctggtcgt gacggccatc gcgcggcgcc gcaggttggc cttcgcgtag gcgatcgcgg
      841 cgtgcgccat tgcctgctcg ttgtgcgcgc gccaggtggg caggcggtcg cgctcggcgt
      901 gcagcgcctc gccgagcccg gccacgttgc cgtgcccgaa gatcgcgaac gcgcctccga
      961 agtagggcac gacgcggccg tcgtcgagct cgacgcgcag cgccgcgaga tggcgcacca
     1021 gggcctgggc gagggtgagt cggatcgtct tcatggggat gtgcgtccgt caggcggcgt
     1081 cgacgccggc cgctgatacg gcaacaggtt ggggtaccgt cccgccctgg ggccgcgtgg
     1141 cgcgccaggc gtcgaccagt tgctcgaagt tggcggcgac ggcggcgacg aaggcctcgt
     1201 cggcggccgt gccgggcggc gccgcgagcc aggcgctcgc cgggccggcc cacagcgagc
     1261 ggcccaccat gaagccccgc accagcggat gcgtcgccgc cgcgaagccg cgcgccaggt
     1321 cgtcgagcgg ctggttgagg ccgaggatga cggcgccgcg gcattgcggg tcgcgcgcgg
     1381 ccaccagcgc ggccaggcgc gtccacgacg gcgcctgcat cggggcgagc ttccaccact
     1441 ccggcttgag gccgatcgtg tacagccgcg tgacggcgcg cagcacggcg gcgtcggccg
     1501 cgggcgtggc cggcgtcatg ccgggcggga tcacctcgag cagcagctcg ttgccgctcg
     1561 cgcgcgtcgc cgcccaggcc tcgcgcaggc gggcttcctg ctccaggcgc aggtcgacgt
     1621 cctcgtcggg gtggtagtgc accaggcact tgacgacctg ctcgcgcggc caggcgacca
     1681 gctgcgaccc gagcgagccg ctcccttcga agcgcagcgg gcgcgagccg ggcagctcga
     1741 tcggccggcc gacccaccag ccgcggcccg tggcggcgtg cagcgcgtcc tcgccgaggc
     1801 tgtcgtcgat cagcacgccg acgcgccc
//
LOCUS       DASVLN010000654         5153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_33344, whole genome shotgun sequence.
ACCESSION   DASVLN010000654 DASVLN010000000
VERSION     DASVLN010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33344"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..137
                     /locus_tag="VF453_15435"
     CDS             18..137
                     /locus_tag="VF453_15435"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7689107.1"
                     /translation="MRLVRDAMAAAGGNQSEAARRLGVSRVGLIKKLTRLGLR"
     gene            244..1038
                     /locus_tag="VF453_15440"
     CDS             244..1038
                     /locus_tag="VF453_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689108.1"
                     /translation="MSARIPRSLAAIAVTLVLAAGAAARAAVPAIAASAPVVAPSTDL
                     ALEVVGMRPPSGSTLKANEPVVVTLRYRYARPAGDVMVWAKLLGAGPSTYEGAHPDTP
                     AGSGTIERTVTLNGPGEVHRLTLVAKDARLQQIYRREVPVHYTWVPNPRAEAIRDDGA
                     DSRIVGVRLAAGTPAVLRAGTPVAVELDVDNRSSRGVQAWVEPVTSCNMAFDDLLKVE
                     AARATVRQRFTVGQKCVVTRLRVALANSAGVRVFDQLVDVDLRYVD"
     gene            complement(1026..1949)
                     /locus_tag="VF453_15445"
     CDS             complement(1026..1949)
                     /locus_tag="VF453_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7689109.1"
                     /translation="MKIENIADLQVLLHAARGGSLSAAARTLGITPAAASATLKRLEA
                     QLGTRLVERSTRAMRLTPPGQILLDYARRAFELLAEGESQLDAERGALVGTVRLAAPS
                     DLTRTVLMPMLDDFLRLHPGVELALNVGDRVLDVLRDEVDLAIRYGELADSRLVARTL
                     TVARPVLSASPDYLRRRGTPRTPLDLVDHNCIAFMRGGRPFRTWRFGQRGQWTTVRVG
                     GDRSVDDASLAREWALAGAGILLKTEIEQRRDLASGALVPLLADWETDPYPLHALLPS
                     GRFVPARVRALVDFLARRFAAPAALSTGQST"
     gene            2044..3054
                     /locus_tag="VF453_15450"
     CDS             2044..3054
                     /locus_tag="VF453_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX7689110.1"
                     /translation="MKAVGYHHNLPIDDPRALEDLELPAPQPGPRDLLVRVKAVSVNP
                     VDTKVRRNTAPPAGQAKVLGWDAVGIVEAVGPQAQGFAVGDRVWYAGSITRPGANSEL
                     HAVDARIVSKAPASVADAQAAALPLTAITAWELLFDRLRVPQGGGAGQTLLVVGGAGG
                     VGSILIQLARQLTQLRVVATASRPESREWCLALGAHAVIDHSQPLAEALKAAGLGQVD
                     YVASLTQTPRHYAQIVESLKPQGALGVIDDMKGLEVDLLKTKALSLHWELMFTRPMFE
                     TPDIAEQGRLLAQVAALVDAGRLRTTANAAFGRITAANLARAHALVESGKAVGKVVLE
                     GF"
     gene            3099..3407
                     /locus_tag="VF453_15455"
     CDS             3099..3407
                     /locus_tag="VF453_15455"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7689111.1"
                     /translation="MVGGLGISGGNAQQDHDTALAALAPPAFEALPGSGQAIALRAED
                     LHVQRFGDAAIVTGVRVGAVGPTAGSTSPEPRVPFTMVWTKDADGWRLRHAHLSAPLP
                     "
     gene            complement(3435..4421)
                     /locus_tag="VF453_15460"
     CDS             complement(3435..4421)
                     /locus_tag="VF453_15460"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7689112.1"
                     /translation="MQVTTASASASERSRRSANDKIDNRILHVLQRDGRISNLKLAEQ
                     VNLSPSAVLERVKRLKREGWILGYQARLSREKLGMEVVAFVEVQLERMSSELMERFEA
                     AVRERPAILECHMIAGTFDYLLKVLVPDMHACRELVESEIAKLPGVREARTHAGVEGE
                     PGSRSSALGGPGGATEQRIDAIDAGLLRLLQADGRLSIARLAEELDLTPAVVRERLAR
                     LCRKGVILGYVAVLNEAKFNSGLLVFSAVRVSGSHSGIAHALKGATQRCPEIVECHEV
                     TGNFDFLLKTRVPNMARYHELIESTVWTLAGVREVRTYAAIDEIKNTARIPL"
     gene            4744..>5153
                     /locus_tag="VF453_15465"
     CDS             4744..>5153
                     /locus_tag="VF453_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689113.1"
                     /translation="MRLKSLRRAAPVLIAAATLALVVLSASVAYVWSERHDFARLDDS
                     VSHELDLYAAVLDIELGKQADLPGLIDQDDRIDALVHTPGAADLRALINRKLTRFVAR
                     SGALQATIVDADAHVIASSDWYRAGARPGAPAASG"
BASE COUNT          686 a         1916 c         1851 g          700 t
ORIGIN      
        1 cgctcggcga gctggagctg cgcctcgtgc gcgacgcgat ggcggccgca ggcggcaacc
       61 agtccgaggc ggcgcggcgg ctgggcgtgt cgcgcgtcgg cctgatcaag aaactgaccc
      121 gccttggcct gcgttgaggc cagaggcccc gcggcatcgg ccggcgcgat ctgccaacta
      181 atgcgcatgc tccgccgcgg cgtcgccggg cggtctgctc gacagcgcag aatcgccgct
      241 cccatgtccg cccgaatccc tcgttcgctc gccgccatcg ccgtcacgct cgtcctcgcg
      301 gccggcgcgg ctgcgcgcgc ggcggtgccg gccatcgcgg cgtcggcgcc cgtggtcgcg
      361 ccgtcgaccg acctcgcgct ggaggtggtc ggcatgcgcc cgccgtcggg cagcaccctc
      421 aaggccaacg agccggtcgt cgtcacgctg cgctaccgct atgcgcggcc cgcgggcgac
      481 gtgatggtgt gggccaagct gctcggagcc ggtccgtcga cctacgaggg cgcgcatccc
      541 gacacgcctg ccggcagcgg caccatcgag cgcaccgtca cgctcaacgg cccgggcgag
      601 gtgcaccggc tcacgctggt cgccaaggat gcgcgcctgc agcagatcta ccgccgcgag
      661 gtgccggtgc actacacctg ggtgcccaat ccgcgcgccg aggcgatccg cgacgatggc
      721 gccgactcgc gcatcgtcgg cgtgcgcctg gccgcgggca cgccggccgt gctcagggcc
      781 ggcacgccgg tggccgtgga gctggacgtc gacaaccgct cctcgcgcgg cgtgcaggcc
      841 tgggtcgagc cggtcacctc gtgcaacatg gccttcgacg acctgctcaa ggtcgaggcg
      901 gccagggcca ccgtgcgcca gcgcttcacc gtcggccaga agtgcgtcgt cacgcggctg
      961 cgcgtggcgc tcgccaacag cgccggcgtg cgcgtgttcg accagctcgt ggacgtcgac
     1021 ctgcgctacg tcgactgacc ggtcgacagg gccgccggcg ccgcgaagcg ccgtgccagg
     1081 aaatcgacca gcgcgcgcac ccgcgccggc acgaagcggc cgctgggcag cagcgcgtgc
     1141 agcgggtacg ggtcggtctc ccagtcggcg agcagcggca ccagcgcgcc gctggcgagg
     1201 tcgcggcgct gctcgatctc ggtcttgagc aggatgcccg cgccggcgag cgcccactcg
     1261 cgcgcgagcg aggcgtcgtc gacgctgcgg tcgccgccca cgcgcaccgt cgtccactgg
     1321 ccgcgctggc cgaagcgcca ggtgcggaac ggccggccgc cgcgcatgaa ggcgatgcag
     1381 ttgtggtcga cgaggtccag cggcgtgcgc ggcgtgccgc ggcggcgcag gtagtccggc
     1441 gaggcgctca gcacgggccg cgccaccgtc agcgtgcgcg cgaccagccg cgagtcggcg
     1501 agctcgccgt agcggatcgc gaggtcgacc tcgtcgcgca gcacgtccag cacgcggtcg
     1561 ccgacgttga gcgcaagctc cacgcccgga tgcaggcgca ggaagtcatc gagcatcggc
     1621 atcagcaccg tgcgcgtgag gtcggacggc gccgccaagc gcaccgtccc gaccagcgcc
     1681 ccgcgctcgg cgtccagctg cgactcgccc tcggccagca gctcgaaggc gcggcgcgcg
     1741 tagtccagca ggatctggcc gggcggcgtc aggcgcatcg cgcgggtcga gcgctcgacc
     1801 agccgcgtgc ccagctgggc ctccaggcgc ttgagcgtgg cgctcgccgc cgccggcgtg
     1861 atgcccagcg tgcgcgcggc ggcgctcagc gagcccccgc gcgccgcatg cagcagcacc
     1921 tggaggtcgg cgatattttc aattttcatt tgaaggtgaa atcctcttca acgatcttat
     1981 caactgccgc ttggatgcct acagtgcatc ccatcgacat cgcatccctt cccaggagtc
     2041 cccatgaaag ccgtcggcta ccaccacaac ctgcccatcg acgacccccg cgcgctcgag
     2101 gacctggaac tgcccgcccc ccagcccggg ccgcgcgacc tgctggttcg ggtcaaggcc
     2161 gtgtccgtga acccggtcga caccaaggtg cgccgcaaca ccgcgccgcc ggccggccag
     2221 gccaaggtgc tggggtggga cgccgtcggc atcgtcgagg cggtcgggcc gcaggcgcag
     2281 ggcttcgcag tcggcgaccg ggtctggtac gccggctcga tcacccgccc gggcgcgaac
     2341 agcgagctgc acgccgtcga cgcgcgcatc gtctcgaagg cgcccgcctc ggtcgcagac
     2401 gcccaggccg ccgccctgcc gctgaccgcg atcaccgcgt gggagctgct gttcgaccgc
     2461 ctgcgcgtgc cgcagggagg cggcgccggg cagacgctcc tggtcgtcgg cggcgccggc
     2521 ggcgtcggct cgatcctgat ccagctcgcg cgccagctga cgcagctgcg cgtcgtggcg
     2581 accgcctcgc gccccgagtc gcgcgagtgg tgcctcgcgc tcggcgcgca cgccgtgatc
     2641 gaccattcgc aaccgctcgc cgaggcgctg aaggcggcag ggctagggca ggtcgactac
     2701 gtggcctcgc tcacccagac gccgcggcac tacgcgcaga tcgtcgagag cctgaagccg
     2761 cagggcgcgc tgggcgtgat cgacgacatg aagggcctgg aggtcgacct gctcaagacc
     2821 aaggcgctgt cgctgcactg ggagctcatg ttcacgcggc cgatgttcga gacgcccgac
     2881 atcgccgagc agggccggct gctcgcgcag gtcgcggcgc tcgtcgacgc gggccgcctg
     2941 cgcacgacgg ccaatgccgc cttcggccgc atcacggccg cgaacctcgc gcgcgcgcat
     3001 gcgctcgtgg aaagcggcaa ggccgtcggc aaggtcgtgc tcgaaggctt ctgacgacgg
     3061 cgcccgcctt cggcctcgac acgcagacct ggggcggcgt ggtgggcggc ctcggcatct
     3121 cgggcggcaa cgcgcagcag gaccacgaca ccgcgctcgc ggcgctcgcg ccgccggcgt
     3181 tcgaggcgct gcccggctcc ggccaggcga tcgcgctgcg cgccgaggac ctgcacgtgc
     3241 aacgcttcgg cgacgcggcg atcgtgaccg gcgtgcgcgt gggcgcggtc gggccgacgg
     3301 caggatcgac gtccccggag ccgcgcgtcc cattcacgat ggtgtggacg aaggacgccg
     3361 acggctggcg cctgcgccac gcgcacctgt cggcgccctt gccctgaagc gcggcgtgcc
     3421 gcgtccggcc gcgctcagag cgggatgcgc gccgtgttct tgatctcgtc gatcgccgcg
     3481 taggtgcgca cctcgcgcac gccggccagc gtccagacgg tcgattcgat cagctcgtgg
     3541 tagcgcgcca tgttgggcac gcgcgtcttc agcaggaagt cgaagttgcc ggtgacctcg
     3601 tggcattcga cgatctccgg gcagcgctgc gtcgcgccct tgagcgcgtg cgcgatcccc
     3661 gagtgcgagc ccgacacccg caccgccgag aacacgagca ggccggaatt gaacttggcc
     3721 tcgttgagca cggcgacgta gccgaggatc acgcccttgc ggcagagtcg ggcgaggcgc
     3781 tcgcgcacca cggcgggcgt caggtcgagc tcctcggcca ggcgcgcgat gctcaggcgg
     3841 ccgtcggcct gcagcaggcg cagcaggccg gcgtcgatgg cgtcgatgcg ctgctcggtc
     3901 gcgccgccgg ggccgccgag ggcgctgctg cggctgccgg gctcgccctc gacgcccgcg
     3961 tgggtgcgcg cctcgcgcac cccgggcagc ttggcgatct cggactccac gagctcgcga
     4021 caggcgtgca tgtcgggtac gagcaccttc agcaggtagt cgaaggtgcc ggcgatcatg
     4081 tggcactcca ggatggccgg ccgttcgcgc accgcggcct cgaagcgctc catcagctcg
     4141 gaactcatgc gctcgagctg cacctccacg aaggcgacca cctccatgcc cagcttctcg
     4201 cggctgaggc gcgcctggta gcccaggatc caaccctcgc gcttcaggcg cttgacgcgc
     4261 tccagcaccg cggagggcga caggttgacc tgctcggcga gcttcaggtt ggagatccgt
     4321 ccgtcgcgct gcagcacgtg gaggatgcgg ttgtcgatct tgtcgttggc gctgcgccgg
     4381 gagcgctcgg aagcggaagc ggaggcggtg gtgacttgca tgttcgacgg ctagggtgga
     4441 tggcttgtct cgtgaccccg ggtcggacgc ccggtcgcgg actccatatg cacccgccgt
     4501 gccagctcga cggcggatcc gtgtaaagcg ccgttccacc tctgtaaccg gccgtgcacg
     4561 gcggcggggg ccggcgccgg gccgtgtgaa gttctaccca cggcgccgcc gcggcgtgta
     4621 tcggtccccg ctcacggccc cgcggcggcg cttgaccgcg gggcggccgg gcctcaacat
     4681 cgggcgcttt cgtacgcgac agcgcccgcc agaggtgccg ttcccgccgc tcccttggat
     4741 ccgatgcgac tgaagtccct gcgccgcgca gcgcccgtgc tgatcgcggc ggcgacgctc
     4801 gcgctcgtcg tgctgtcggc gagcgtcgcc tacgtctggt cggagcgcca cgacttcgcg
     4861 cgcctggacg actccgtctc gcacgagctc gacctctacg ccgccgtgct cgacatcgag
     4921 ctcggcaagc aggccgacct gcccggcctg atcgaccagg acgaccgcat cgacgcgctg
     4981 gtgcacacgc ctggcgcggc cgacctgcgg gccctcatca accgcaagct cacgcgcttc
     5041 gtcgcccgtt ccggcgcgct gcaggccacc atcgtcgacg ccgacgccca cgtgatcgcg
     5101 agcagcgact ggtaccgcgc cggcgcccgc cccggcgcgc ccgcggccag cgg
//
LOCUS       DASVLN010000655         3471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_465250, whole genome shotgun sequence.
ACCESSION   DASVLN010000655 DASVLN010000000
VERSION     DASVLN010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465250"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..442)
                     /locus_tag="VF453_15470"
     CDS             complement(71..442)
                     /locus_tag="VF453_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689114.1"
                     /translation="MIRTLVAALAALCTLAAAAAQAAPAAPANGVLVTPTGATLYTFD
                     KDVAGSGKSACNGPCAEAWPPFKAAASDEAAGDWTIVVREDGSRQWAYKGWPLYTFSK
                     DAKPGDKSGDNFKNIWHVIKE"
     gene            724..2445
                     /locus_tag="VF453_15475"
     CDS             724..2445
                     /locus_tag="VF453_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7689115.1"
                     /translation="MTAIDPSAAPGARAFLEARDFLLRHREDYATAYRDFRWPALDTF
                     NWALDYFDPFARGNEAPALWIVDESGGEEKVSFDRMARRSSQLANWLRGLGVRRGERV
                     LLLMPNEVALWESMLACMKLGAVIVPTTTLVAADDLADRFDRGAVRHVIATTAACAKF
                     DEFPGAYTRVAVPEAGGEPVPMGWHSIAESLSAPEDFAADAPTRADDPLFLYFTSGTT
                     SRPKLVLHSHQSYPVGHLSTMYWIGLKPGDVHWNISSPGWAKHAWSCFFAPWNAGATI
                     FIYNYARFNAPAALDVLVRNRVTSLCAPPTVWRMLMQQDLAAYRTSLRELVGAGEPLN
                     PEVIDIVRRAWGITIRDGFGQTETTAQVGNPPGQPLKPGSMGRPLPGYDVALLDADGR
                     PADEGEIALTLAPRPLGLMLGYQDDDARTSEAMRDGHYHTGDVAVRDADGYITYVGRA
                     DDVFKASDYRISPFELESVLIEHEAVAEAAVVPSPDPIRLAVPKAIVVLRAGHAPSPE
                     LAQSILAFARSRLASYKRIRIVEFRELPKTLSGKIRRVELRLREKGRAPGEAWPQEFR
                     EPEKTSE"
     gene            2505..2948
                     /locus_tag="VF453_15480"
     CDS             2505..2948
                     /locus_tag="VF453_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7689116.1"
                     /translation="MQIRRLNAADQALARELFVTMAGVFGEDSDKLSDDYLARLLARD
                     GFWAIAAIDGEQVVGGLTAHTLPMTATETSVVFLYDLAVRRGHQRRGVGRLLVQALRE
                     AAKAAGASEVFVGADNADEQALGFYRAMGGEAALATFFSFPLHKA"
     gene            3051..>3471
                     /locus_tag="VF453_15485"
     CDS             3051..>3471
                     /locus_tag="VF453_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689117.1"
                     /translation="MRLSGKTLRRAAASAAAAVLVAGAPAAHADRQWHWQFGGADGLE
                     ASGTFITGNTPDASGFYLITAISGERDGVAITGLQAAGTAIPGNEPYAVDDLVSPTGP
                     QLTGNGFGFTTADGNASNPFWADFDTPADYLEFRSFGP"
BASE COUNT          467 a         1298 c         1231 g          475 t
ORIGIN      
        1 ggcgccgcga cgggccgcgg gacgtccgcc ggcgccggcg gatccggccg cgacggcgcg
       61 gtcatcgccg tcactccttg atcacgtgcc agatgttctt gaagttgtcg ccggacttgt
      121 cgcccggctt ggcgtccttg gagaacgtgt acagcggcca gcccttgtag gcccactggc
      181 gcgagccgtc ctcgcgcacg acgatcgtcc agtcgcccgc ggcctcgtcc gaggcggcgg
      241 ccttgaacgg cggccaggcc tcggcgcagg ggccattgca ggcgctcttg ccgctgccgg
      301 caacgtcctt gtcgaaggtg tagagcgtgg cgccggtcgg cgtgaccagc acgccgttgg
      361 cgggtgccgc gggggcggcc tgcgcggcgg cggccgcgag ggtgcacagc gccgcgagcg
      421 cggcgacgag ggtcctgatc atgcgtggtg ctcctgtgga cgggccgccg gccgcggacg
      481 gcgacggcat cttgcagggt ggacgtgaga ccttgcccga gtcccgcgtt gtacaggatt
      541 ttccgggccc gcgctcgtac gcgttttcca cgtgcgtcgc ggcccccggc tgtcagcgcg
      601 gagtagggag cccggggcta tcctagcgtt caccgcggcc cgcccgaggg gcggccgccg
      661 acgacgacca tgccgccccg ccgccatccg cgcggcacga ggcggccaac ggagacaacg
      721 cccatgaccg ccatcgaccc gtccgccgcg cccggcgctc gcgccttcct ggaggcgcgc
      781 gacttcctgc tccggcaccg cgaggactac gccaccgcgt accgcgactt ccgctggccc
      841 gcgctcgaca cgttcaactg ggcgctcgac tacttcgacc ccttcgcccg cggcaacgag
      901 gcgccggcgc tgtggatcgt cgacgagtcc ggcggcgagg agaaggtgag cttcgaccgc
      961 atggcgcggc gctcgtcgca gctcgcgaac tggctccgcg ggctcggcgt ccggcgcggc
     1021 gagcgcgtgc tgctgctgat gcccaacgag gtggcgttgt gggagagcat gctcgcctgc
     1081 atgaagctcg gcgccgtgat cgtgccgacg acgacgctcg tcgccgccga cgacctcgcg
     1141 gaccgcttcg accgcggcgc cgtcaggcac gtgatcgcca ccacggccgc ctgcgccaag
     1201 ttcgacgagt ttcccggcgc ctacacccgc gtcgcggtgc ccgaggcggg cggcgagccg
     1261 gtgcccatgg ggtggcactc gatcgccgag tcgctgtccg cgcccgagga cttcgccgcc
     1321 gacgccccga cgcgggcgga cgacccgctg ttcctctact tcacctcggg cacgacctcg
     1381 cggcccaagc tcgtgctgca cagccaccag agctaccccg tggggcacct gtcgacgatg
     1441 tactggatcg gcctgaagcc gggcgacgtg cactggaaca tcagctcgcc gggctgggcc
     1501 aagcatgcgt ggagctgctt cttcgcgccg tggaacgccg gcgcgacgat cttcatctac
     1561 aactacgcgc gcttcaacgc gccggccgcg ctggacgtgc tcgtgcgcaa ccgcgtcacc
     1621 tcgctgtgcg cgccgccgac cgtctggcgc atgctgatgc agcaggacct cgccgcgtac
     1681 cggacctcgc tgcgcgagct cgtgggcgcg ggcgagccgc tcaatccgga ggtgatcgac
     1741 atcgtgcgcc gcgcctgggg catcaccatc cgcgacggct tcgggcagac cgagacgacg
     1801 gcgcaggtcg gcaatccgcc ggggcagccc ctcaagcccg gctcgatggg ccggcccctg
     1861 cccggctacg acgtcgcgct gctggacgcc gacggccggc ccgccgacga gggcgagatc
     1921 gcgctgacgc tcgccccgcg gccgctgggc ctgatgctgg gctaccagga cgacgacgcg
     1981 cggacctccg aggcgatgcg cgacggccac taccacacgg gcgacgtcgc cgtgcgcgac
     2041 gccgacggct acatcaccta cgtcggccgc gccgacgacg tgttcaaggc ctctgactac
     2101 cgcatcagcc cgttcgagct cgagagcgtg ctgatcgagc acgaggccgt cgccgaggcc
     2161 gcggtcgtgc cctcgccgga cccgatccgc ctggccgtgc ccaaggccat cgtcgtcctg
     2221 cgcgccggcc acgcgccgag ccccgagctc gcgcagtcca tcctcgcgtt cgcgcgctcg
     2281 cgcctggcgt cgtacaagcg catccgcatc gtcgagttcc gcgagctgcc caagacgctg
     2341 tcgggcaaga tccggcgcgt ggagctgcgc ctgcgcgaga aggggcgcgc gcccggcgag
     2401 gcctggccgc aggagttccg cgagccggag aagacttcgg agtaaaaagg cgggatcgtc
     2461 gatcccggcc gcgcgagccg cgccccaccc cggaaaggaa acgcatgcag atccgcagac
     2521 tcaacgccgc cgaccaggcc ctcgcgcgcg agctgttcgt cacgatggcc ggcgtgttcg
     2581 gcgaggacag cgacaagctg tccgacgact acctcgcccg cctgctggcg cgcgacggct
     2641 tctgggcgat cgcggcgatc gacggcgagc aggtcgtcgg cggcctcacc gcgcacacgc
     2701 tgccgatgac ggccaccgag acctcggtcg tcttcctcta cgacctcgcc gtgcggcggg
     2761 gacaccagcg ccgcggcgtc ggccggctgc tggtgcaggc gctgcgcgag gccgcgaagg
     2821 ccgccggcgc gagcgaggtc ttcgtcggcg ccgacaacgc ggacgaacag gcgctcggct
     2881 tctaccgggc gatgggcggc gaggcggcgc tcgcgacctt cttctcgttc ccgctgcaca
     2941 aggcctgagg ggccccgcgc gccgatgccc gcatgccggg gtggggtatc gcggccgcgc
     3001 ggcgacgccg tctcttacag tgccggcttt cgaacgcaag cggagcgaac atgagactca
     3061 gtggcaagac cctccgccgg gcggcggcgt ccgcggcggc ggccgtcctc gtcgccggcg
     3121 cgccggccgc gcatgccgat cgccagtggc actggcagtt cggcggcgcc gacggcctcg
     3181 aggcctcggg caccttcatc accggcaaca cgcccgatgc gtcgggcttc tacctcatca
     3241 ccgcgatctc gggcgagcgc gacggcgtgg ccatcaccgg cctgcaggcg gctggcacgg
     3301 cgatccccgg caacgagccc tacgccgtgg acgacctggt ctcgccgacc ggcccgcagc
     3361 tgacgggcaa cggcttcggc ttcacgaccg cggacggcaa cgcgagcaat ccgttctggg
     3421 cggacttcga cacgccggcc gactatctcg agttccgctc gttcggcccg g
//
LOCUS       DASVLN010000656         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_33369, whole genome shotgun sequence.
ACCESSION   DASVLN010000656 DASVLN010000000
VERSION     DASVLN010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33369"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..2394)
                     /locus_tag="VF453_15490"
     CDS             complement(88..2394)
                     /locus_tag="VF453_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7689118.1"
                     /translation="MQSVTVTGNGRAQQLQNVPIAVQIVDAAQIDKLAASNLGDMNGF
                     IPGLHVNAEQPTQPFFVMRGIGTGDFGIGTDAPVGVYVDGVYTGKTGGALLNFNDVRR
                     VEVLEGPQGTLFGRNSAGGAISVVTNDPSGNFAANGLLRLGSWGTKHVEAMVNDPLNE
                     DFALRVSAVGQFSDGWQRESATGQRERGDHAWGTRTALRWSPSTETDVVLSWEHEDLK
                     QRAMPAIGLLATPAFGADPSTYLDPRKAPLHNDAAGDGESRRFDGLTLRVDRSLGWAD
                     FTSTSAMRHFTSRNVADNDGTANEATTLTTGNFERNTTVQQEFKLAGKNDAVDWLGGA
                     SLFWERADQTSAIYTNTDSLDTLYGNVAGVAPFQTIDALAQGAGVDGIDFSRQDWQED
                     MVNQGRYRAFALYGDAIWHLGTKTNLTTGLRLTHDDKRFSWYSPPRSAPGVDAQLAAL
                     AAGDFFPSLVAAGALSQGDADTLQALATQGRLIDGTGAQSAPLAWHKSWNNASPRLVL
                     DHHLSPDTMVYASVTRGYQSGGFNALQVGGAYSPETVTSYELGAKGQWPAGGLSYSVA
                     FFHYDYKNLQSLTLVASDTPGGIPVYAVTHSDERADGLDLDLRWRATSNVTLFGAAEF
                     IDQKYAHDTASDGTDLSGQPVGTPRVTATAGLDVAWALAGGKADATLQGSYIGATRCN
                     DDSVAQGTCLATPTLRVGAPQTRVDARLGWDSAPAGKPGWGVALVANNLANRRYITEV
                     NYVAAPLGSPWATITPPRFFGIEFRGVM"
BASE COUNT          319 a          894 c          915 g          383 t
ORIGIN      
        1 ttcgcctcgt cccaccggcc cgcgcccggc cgcccgaagc gggccggtgg agccccccgg
       61 gggcggcgag cggcagcgag cccgggctca catgactccg cggaactcga tgccgaagaa
      121 ccgcggcggc gtgatcgtcg cccacggcga gcccagcggc gccgcgacgt agttcacctc
      181 ggtgatgtag cgccggttcg cgaggttgtt cgccaccagc gcgacgcccc agccaggctt
      241 gcccgcgggc gcgctgtccc agcccaggcg ggcatcgacg cgggtctgcg gcgcgccgac
      301 gcgcaaggtg ggcgtcgcca ggcaggtgcc ttgcgcaacc gagtcgtcgt tgcatcgcgt
      361 cgcgccgatg taggagccct gcagcgtcgc gtccgccttt ccgccggcca gcgcccacgc
      421 gacgtcgagg ccggcggtgg ccgtcacgcg cggcgtgccc acgggctggc cgctcaggtc
      481 ggtgccgtcg ctggcggtgt cgtgggcata tttctggtcg atgaactcgg ccgcgccgaa
      541 cagcgtgacg ttcgacgtcg cgcgccagcg cagatcgagg tcgaggccgt cggcgcgctc
      601 gtcgctgtgc gtgaccgcgt agaccgggat gccgcccggg gtatcggagg cgaccagcgt
      661 caacgactgc aggttcttgt agtcgtagtg gaagaacgcg acgctgtagc tcaggccgcc
      721 cgcgggccac tggcccttcg ccccgagttc gtagctggtc acggtctccg gcgagtaggc
      781 gccgccgacc tgcagcgcgt tgaagccgcc ggactggtag ccgcgcgtga ccgaggcgta
      841 gaccatcgtg tccggcgaca ggtggtggtc gagcacgagg cgcgggctcg cgttgttcca
      901 cgacttgtgc cacgcgagcg gcgccgactg cgcgcccgtg ccgtcgatca gccgcccctg
      961 cgtcgccagc gcctgcagcg tgtcggcatc gccctgcgac agcgcacccg ccgcgaccag
     1021 cgaagggaag aagtcgcccg ccgccagcgc cgcgagctgc gcgtccacgc ccggcgcgct
     1081 gcgcggcggg ctgtaccagc tgaagcgctt gtcgtcgtgc gtcaggcgca ggccggtcgt
     1141 gaggttggtc ttcgtgccga ggtgccagat cgcgtcgcca tacagcgcga acgcgcggta
     1201 gcggccctgg ttcaccatgt cctcctgcca gtcctggcgc gagaagtcga tgccgtccac
     1261 gcccgcgccc tgcgcgagcg catcgatcgt ctggaacggc gcgacgcccg cgacgttgcc
     1321 gtagagcgtg tcgagcgaat cggtgttggt atagatcgcg ctggtctggt cggccctctc
     1381 ccagaagagg ctcgcgccgc ccagccagtc gaccgcgtcg ttcttgccgg cgagcttgaa
     1441 ctcctgctgc acggtggtgt tgcgctcgaa gttgccggtc gtcagcgtcg tcgcctcgtt
     1501 ggcggtgccg tcgttgtcgg cgacgttgcg cgacgtgaag tggcgcatcg cgctcgtcga
     1561 cgtgaaatcg gcccagccga gcgaacggtc gacgcgcagc gtcaggccgt cgaagcggcg
     1621 cgactcgccg tcgccggccg cgtcgttgtg cagcggcgcc ttgcgcgggt cgaggtaggt
     1681 cgacggatcg gccccgaacg cgggcgtcgc gagcaggccg atcgcgggca tcgcacgttg
     1741 cttcagatcc tcgtgctccc acgacagcac gacgtccgtc tcggtggacg gcgaccagcg
     1801 cagcgcggtg cgcgtgcccc acgcgtggtc gccgcgctcg cgctggccgg tcgcggactc
     1861 gcgctgccag ccgtcgctga actggccgac ggcgctcacg cgcagggcga agtcctcgtt
     1921 cagcgggtcg ttgaccatcg cctcgacgtg cttggtgccc cagctgccca gccgcagcag
     1981 cccgttggcc gcgaagttgc cgctcgggtc gttggtgacg accgagatcg cgccgcccgc
     2041 gctgttgcgc ccgaacagcg tgccctgcgg accctcgagc acctcgacgc gcctgacatc
     2101 gttgaagttc agcagcgcac cgccggtctt gccggtgtag acgccgtcga cgtacacgcc
     2161 caccggcgca tcggtgccga tgccgaagtc gccggtgccg atgccgcgca tcacgaagaa
     2221 gggctgcgtc ggctgctccg cgttcacgtg caggccgggg atgaagccgt tcatgtcgcc
     2281 caggttggag gcggcgagct tgtcgatctg cgccgcgtcc acgatctgca ccgcgatcgg
     2341 cacgttctgc agctgctgcg cgcggccgtt gccggtgacg gtgaccgatt gcaccgcctg
     2401 cgcgtccgta cccctgccgg cagccgtatc ggccggcgcg ctggcggcag cggccggcgc
     2461 gggcgcggac gactgcgccc atgcggaggg ggcgagcagc gcggccagcg c
//
LOCUS       DASVLN010000657         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_304030, whole genome shotgun sequence.
ACCESSION   DASVLN010000657 DASVLN010000000
VERSION     DASVLN010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304030"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..414)
                     /gene="sctS"
                     /locus_tag="VF453_15495"
     CDS             complement(154..414)
                     /gene="sctS"
                     /locus_tag="VF453_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctS"
                     /protein_id="HEX7689119.1"
                     /translation="MDVDSVLRLTSQAMILCLMVSLPAVVVSAVVGLLASFVQTITSL
                     QDQAISQGAKLIAVVIALLISAPWGASMLLGFARQALDAALR"
     gene            complement(436..1095)
                     /gene="sctR"
                     /locus_tag="VF453_15500"
     CDS             complement(436..1095)
                     /gene="sctR"
                     /locus_tag="VF453_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HEX7689120.1"
                     /translation="MTPTSSAAEVVPLILLVVAIGLVPFVAMMVTSYTKIVVVLGLLR
                     NALGVQQVPPNMVLNGIAIIVSIYIMAPVGMDTFDRIRADGVAPTTGAQMAQLMTAAE
                     DPVRRFLVRHAQPREKAFFLKSAAQVWPEERAKALQADDLIVLAPAFTLTELTSAFRV
                     GFLIYLAFVVVDLVIANVLLALGLSQVTPTQVAIPFKLLLFVVMDGWSTLLHGIVLGY
                     R"
     gene            complement(1070..>1555)
                     /locus_tag="VF453_15505"
     CDS             complement(1070..>1555)
                     /locus_tag="VF453_15505"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX7689121.1"
                     /translation="IATRRIALGVLRSLEPGDVVLPEQALESLEGAPATFRMEAGAGR
                     GLAASAQVADGRLVLLGVPRMTDDDALAAPPTGATAIDELELPLQFELETVSITLAEL
                     ESIEPGYVIEFDTPATQARLRLVSCGVVVGEADLVAVGGRLGACITHLAPRHDADQQR
                     S"
BASE COUNT          259 a          528 c          570 g          198 t
ORIGIN      
        1 tggtgcccga gcggatcgcg ctccttcgcg tcgggctcgg tcgcggggct ggccggcgcc
       61 accggcgcgc tggcgtgagc cgccgcgcgg gccgccacgc catggttcaa gcggttgatg
      121 gccacgatgg ggtctccttc gggcgatgcg gggtcaacgc agggcggcgt ccagcgcctg
      181 gcgtgcgaag cccagcagca tggaggcgcc ccagggcgcc gagatgagca gcgcgatcac
      241 caccgcgatg agcttggcgc cctgcgagat cgcctggtcc tgcagcgagg tgatcgtctg
      301 cacgaacgac gccagcagcc ccaccacggc ggacaccacc acggccggca acgacaccat
      361 gaggcacagg atcatcgcct gcgaggtcag gcgcagcacc gaatcgacgt ccatgtcgga
      421 tcctcccggc gacggtcagc ggtagcccag cacgatgccg tgcagcagcg tcgaccagcc
      481 gtccatcacc acgaacagca ggagcttgaa cgggatcgcc acctgcgtcg gcgtcacctg
      541 ggacaggccg agcgcgagca gcacgttggc gatgacgagg tccaccacga cgaaggccag
      601 gtagatcagg aagccgacgc ggaaggcgga ggtcagctcc gtcagcgtga aggcgggcgc
      661 gagcacgatc aggtcgtcgg cctgcagcgc cttggcgcgc tcctcgggcc agacctgggc
      721 ggccgacttc aggaagaacg ccttctcgcg cggctgcgca tggcggacca ggaagcggcg
      781 aaccggatcc tccgccgcgg tcatcagctg cgccatctgc gccccggtcg tcggggccac
      841 gccgtcggcg cgtatacggt cgaacgtatc catgcccacg ggcgccatga tgtagatcga
      901 cacgatgatc gcgatgccgt tgagcaccat gttgggcggt acctgctgga cgccgagcgc
      961 gttgcgcaac aggcccagca cgacgacgat cttcgtgtag gacgtgacca tcatcgccac
     1021 gaacggcacc agcccgatcg cgacgaccag gaggatcagc gggacgacct cagctgcgct
     1081 gctggtcggc gtcatggcgc ggtgccaggt gggtgatgca ggcgcccagg cggccgccga
     1141 ccgccacgag gtcggcctcc ccgacgacga cgccgcacga cacgaggcgc aggcgtgcct
     1201 gggtggccgg ggtgtcgaac tcgatcacgt agccgggctc gatggactcg agctcggcga
     1261 gcgtgatcga gaccgtctcg agctcgaact gcaacggcag ctcgagctcg tcgatggccg
     1321 tcgcgccggt gggcggtgcg gcgagggcgt cgtcgtcggt catgcggggt actcccagga
     1381 ggacgaggcg gccgtcggcc acctgcgcgg atgcagcgag gccgcgaccc gcgccggcct
     1441 ccatgcggaa cgtggccgga gcgccttcga gcgactcgag cgcctgctcg ggcaggacga
     1501 cgtcgccggg ctccagcgag cgcagcacgc cgagggcgat gcggcgcgtg gcgat
//
LOCUS       DASVLN010000658        12059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_33402, whole genome shotgun sequence.
ACCESSION   DASVLN010000658 DASVLN010000000
VERSION     DASVLN010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12059
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33402"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..667)
                     /locus_tag="VF453_15510"
     CDS             complement(80..667)
                     /locus_tag="VF453_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689122.1"
                     /translation="MIRSFTPVVLAAAAGLLCGCVAEPLPKPVVGYTCCNLSPVYGAW
                     ISSNNVQGGTRLPAGQKAHFESIKRTYYVYGQIGGQDYGLRNDSGHDEKSTLEFLLRT
                     IVPEDPNVALATWPADVQAAVHAAKVQVGMTRPQVAMALGYPSPEDTPKLDDPVWRYW
                     VIVGDQRIELHFDAEGKLAAVTGKPEAVAEVFKAS"
     gene            complement(906..2063)
                     /gene="nagZ"
                     /locus_tag="VF453_15515"
     CDS             complement(906..2063)
                     /gene="nagZ"
                     /locus_tag="VF453_15515"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEX7689123.1"
                     /translation="MARADHKPQTKNHRDDDRDDSAEAAAPGIHAPVILDVAGTTLDA
                     ADRRRLAHPLVGGAILFGRNWTNRRQVTELVAEMKAIRPDLLVCVDHEGGRVQRFRTD
                     GFTHLPPMADYGRRWMDDAGGAPGDGAMAATDAATAAGFVLAAELRACGVDLSFAPVL
                     DLDFGHSAVIGDRAFHRDPRVVALLAKSLAAGMRQAGMAHCGKHFPGHGHVAADSHVD
                     IPVDPRRLSEILADDARPYEWLSTSLASVMPAHVVYPAVDDRPAGFSETWLRDILRER
                     LGFTGAIFSDDLSMAGARQLAGRTLEYAQAAVLAMRAGCDLVLLCNQSVGGGKPLDAL
                     LDGLADAQAHGDWVPDALSERRRIDLLPQTEPLAWDDLMHDPAYLRALERL"
     gene            complement(2072..2482)
                     /gene="acpS"
                     /locus_tag="VF453_15520"
     CDS             complement(2072..2482)
                     /gene="acpS"
                     /locus_tag="VF453_15520"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7689124.1"
                     /translation="MTVYGIGTDLCDVRRIAATLERKGDRFAEKVLGPGEIEVFRARR
                     ARVEGRGLRFLATRFSAKEAFSKAIGLGLHMPMTWRDCEILNERSGKPVIRLHGALAE
                     WFEARGLVAHVTVTDETDYVATFVVVETRQLPTA"
     gene            complement(2495..3295)
                     /locus_tag="VF453_15525"
     CDS             complement(2495..3295)
                     /locus_tag="VF453_15525"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX7689125.1"
                     /translation="MNPLVAPEQTRRAHERCALSVNVNKVALLRNTRHLGIPSVVHAA
                     TLALQAGAQGITIHPRPDERHIRAADVPELHALMARWPEAEFNIEGNPFHNLMDFIRA
                     VRPHQATFVPDSEGQFTSDHGWDLARDAARLRPIIEETRSLGVRVSLFMDPVPEAMAL
                     ARAVGADRVELYTEPYAAAHGTPRGAEVLAQFARSAQAALAAGLGVNAGHDLNLANLT
                     DFVRAVPGLAEVSIGHALVADALEFGMTETVHRYLRAIADAEPADSLA"
     gene            complement(3320..4108)
                     /gene="recO"
                     /locus_tag="VF453_15530"
     CDS             complement(3320..4108)
                     /gene="recO"
                     /locus_tag="VF453_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX7689126.1"
                     /translation="MPTARRAAAPLAAYVLHRYDWSETSLILDLFTRERGRIAVAAKG
                     AKRPYSQLRSVLLPFQRIAATVGKQAEEAQADVVTLRGAEWAGDDATPVLPPARLFAG
                     FYLNELLMKLLARGDAHPALFDAYAATLRSLATADEFGEQIGLRAFEFVLLRAIGLLP
                     ELDRVTATQRPLAPAAAYQWLGDRGVIEADPQLGFASLPGADLLALQAALAQESLGPL
                     HVACATRLDRLRPVLRTLVHYHLGSPVLRTREVMRDARHLIDAP"
     gene            complement(4144..5064)
                     /gene="era"
                     /locus_tag="VF453_15535"
     CDS             complement(4144..5064)
                     /gene="era"
                     /locus_tag="VF453_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX7689127.1"
                     /translation="MAAMLADAVDEHGARKRCGLVAIVGRPNVGKSTLMNALVGQKIS
                     ITSRKAQTTRHRIVGVRTDGPAQFVFVDTPGYQMKHSAALNKSLNKTVVSVLTDVDVV
                     LFMVEAGRFGSDDAKALQLLPKGKPAFLVANKLDTVQRRSDLLPWLQQMQQRHPFNEF
                     FPLSSHSKADVDRLAGVIQPYLPEQPWFYDADMLTDRSERFLVSEVIREKLFRLTGDE
                     LPYSATVVIDQFVEEGAMKRIAATVVVERDAHKGMIIGAKGQQLKRIASEARQELERL
                     LESKVFLEVWVKVRSGWADSEEHLRSYGYE"
     gene            complement(5219..5959)
                     /gene="rnc"
                     /locus_tag="VF453_15540"
     CDS             complement(5219..5959)
                     /gene="rnc"
                     /locus_tag="VF453_15540"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX7689128.1"
                     /translation="MTRATPSPAARGAAMVEKRLGYEFAQRALFERALTHRSFGADHN
                     ERLEFLGDAVLNLAVSRLLFDRFSGSDEGDLTRVRAHLVREDSLHRVALALGLPDALR
                     LSEGEARGGGATRPSILADAVEALIGAVSIDGGFDAAQALVARLFGETINATTADNWR
                     KDAKTELQEWLQARHVPVPSYRITATHGQAHAQTFEVECEVPTLKWLARGEGRSRRAA
                     EQASAQHLLDRIKAGELDVPARKGPPSA"
     gene            complement(5967..6362)
                     /locus_tag="VF453_15545"
     CDS             complement(5967..6362)
                     /locus_tag="VF453_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEX7689129.1"
                     /translation="MHPTRRRLHAPQRGTSLFGLFVIAVIVCFIGLMAMRVFPSVNEF
                     LTVRKVVRQIMHDSPTNPAEIRKSFDRAKDVEYSITSISGKDLDIQQVGDHLRCSFAY
                     NVEIPVVEPVFILIKYSGEASAAGGGPNV"
     gene            complement(6416..7381)
                     /gene="lepB"
                     /locus_tag="VF453_15550"
     CDS             complement(6416..7381)
                     /gene="lepB"
                     /locus_tag="VF453_15550"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7689130.1"
                     /translation="MSALTGALYAALVIYLGGWLKDWWPGNFALLLLMLTTVTFLYWL
                     AERLYFRPKREKAAAVLEAQDVARRAELARMGIDKVDGDLQEAKARLLMQPWWLDWTA
                     GLFPVIVVVFVLRSFLFEPFKIPSGSMIPTLQIGDLILVNKFHYGIRLPVINKKIIPN
                     HDPQRGDVMVFHYPIHPEVDYIKRVVGLPGDEVSYLNKQLRINGKLITETPLPDYFVE
                     SSAVYEQAFEEDLDGVKHRILTRSDRPSYVQPFNSFAFKDNCRYNAEGVVCKVPPGHY
                     FMMGDHRDDSEDSRYWGFVPDENIVGRAFFVWMNFGDPSRIGSFH"
     gene            complement(7386..9194)
                     /gene="lepA"
                     /locus_tag="VF453_15555"
     CDS             complement(7386..9194)
                     /gene="lepA"
                     /locus_tag="VF453_15555"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX7689131.1"
                     /translation="MDHIRNFSIIAHIDHGKSTLADRLIQRCGGLSDREMEAQVLDSM
                     DIERERGITIKAQTAALQYQARDGQVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALDLGVEVVPVLNKMDLPSADPDNAKAEIEDVIGIDAEHAIP
                     CSAKTGMGIDDILEAVIARMPPPRGNPDGPPRAMIIDAWFDNYVGVVMLVRVVDGRFA
                     KGERIRLMATNTVYGIEHLGVFTPKSENRDALNAGEVGFVICGIKELAAAKVGDTLTL
                     EKKLPNNAGPADTPLPGFKEIQPQVFAGLYPTEASEYDQLRDALEKLKLNDSSLRFEP
                     EVSQALGFGFRCGFLGLLHMEIVQERLEREFDQDLVTTAPSVVYEVELNDGTVLEVEN
                     PSKMPDQGRIREIREPIVTVHLYMPQDYVGVVMTLCNQKRGVQLNMAYHGKQVMLTYE
                     LPLAEIVLDFFDKLKSVSRGYASMDYEFKEYRSADVVKVDILINGDKVDPLAMIVHRS
                     QSQFRGRAVAAKMREQIPRQMYDVAIQAAIGANIIARENIKALRKNVLAKCYGGDITR
                     KRKLLEKQKAGKKRMKQIGSVEVPQEAFLAILQVDD"
     gene            complement(9746..9821)
                     /locus_tag="VF453_15560"
     tRNA            complement(9746..9821)
                     /locus_tag="VF453_15560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9786..9788),aa:Arg,seq:cct)
     gene            10038..11492
                     /locus_tag="VF453_15565"
     CDS             10038..11492
                     /locus_tag="VF453_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7689132.1"
                     /translation="MGSKFKVAGLLALGAVAGALTTMQIQAIARNTLGSFGSLPLDDI
                     ERFTEVFDRVKTNYVVPVDEKKLIGDAIGGMVSGLDPHSQYFDKKSYKEFQESTSGRF
                     VGVGIEIGMEDGLVKIVTPIEGSPAFRAGLKSGDLITRIDETQVKGLTMDQAVKKMRG
                     DPGSKVVLTIFRKDEGRTFPVTIAREEIHVQSVKAKDFDGYAWVRIAQFQERTVDDFA
                     AKVEDLYKKDPKIKGFVLDLRNDPGGLLDGAIGIASVFLPPDSVIVKTNGQMPDSKQT
                     FKASPEDYRRSGADPLRKLPAALKTVPLVVLINEGSASASEIVAGALQDYKRGTIMGA
                     QSFGKGSVQTVLRLQGDTAIKITTARYYTPSGRSIQEKGIVPEIWLDETAEGNLYSAL
                     FPREEDLDKHLVNDEYKADPAKEKAREAAREAAEAKLAEERAKNGNKPIKPLPDFGSA
                     EDFQLNQALNELRGQPVVVSKTATERKPEDTSTQ"
     gene            11584..>12059
                     /locus_tag="VF453_15570"
     CDS             11584..>12059
                     /locus_tag="VF453_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEX7689133.1"
                     /translation="MTDDDLLRYSRHILLDEIGIEGQRRIAAARALVIGAGGLGSPAA
                     LYLGSAGIGRLVLVDDDAVDLTNLQRQLAHTQDRIGTNKARSARAAVLALNPGLDVVA
                     LERRAGVADLAALAAEADVVLDCSDNFATRQAVNAACVAAGVPLVAGAAIGFDGQVS"
BASE COUNT         1798 a         4088 c         4310 g         1863 t
ORIGIN      
        1 cgggacatgg cgcagtgtag gccgccgcgg gcgccttcac gcccgcgggc ccgggggccg
       61 ccgacccgcc gcctcgggat caggacgcct tgaacacctc ggcgaccgcc tcgggcttgc
      121 ccgtcacggc cgcgagcttg ccctcggcgt cgaagtgcag ctcgatgcgc tggtcgccga
      181 cgatcaccca gtagcgccag accgggtcgt cgagcttggg cgtgtcctcg ggcgatgggt
      241 agcccagggc catcgcgacc tgcggccggg tcatgccgac ctgcaccttg gccgcatgca
      301 cggccgcctg cacgtcggca ggccaggtcg cgagcgcgac gttgggatcc tcggggacga
      361 tggtgcgcag caggaattcc agcgtgctct tctcgtcgtg gccggagtcg ttgcgcaggc
      421 cgtagtcctg gccgccgatc tggccgtaga catagtacgt tcgcttgatc gactcgaagt
      481 gcgccttctg gccggcgggc aggcgcgtgc cgccctggac gttgttgctg gagatccagg
      541 cgccgtagac cgggctcagg ttgcagcagg tgtagccgac gaccggcttg ggcagcggct
      601 cggcgacgca gccgcacagc agccccgccg cggccgcgag cacgaccggc gtgaacgaac
      661 ggatcatggg agtcctcctc ggatgcgatg gcggcggcgc tcgtgcatgg cgccgctcga
      721 ccgccgagta tgcccgcggc cgcggcggcg cggccccggg acaagcacgc gccgcgtgac
      781 gggccgttcg cgccgccgcg cgatgctcgg tggcaccacc acgtcggctg gcgcggcgtc
      841 gatctcgccg atcgggacgc cggcgtcgac gcgggcgaac cccgcatcgc gccggccgca
      901 gcgcctcaga gccgctcgag cgcccgcagg tacgccgggt cgtgcatcag gtcgtcccag
      961 gcgagcggct cggtctgcgg cagcaggtcg atgcggcggc gttccgagag tgcgtcgggc
     1021 acccagtcgc cgtgcgcctg ggcgtcggcc aggccgtcga ggagcgcgtc cagcggcttg
     1081 cccccgccca cgctctggtt gcacagcagg acgaggtcgc aacccgcgcg catcgcgagg
     1141 acggcggctt gcgcgtactc cagcgtgcgc ccggcgagct ggcgcgcgcc ggccatgctc
     1201 aggtcgtcgc tgaagatggc gccggtgaag ccgaggcgct cgcgcaggat gtcgcgcagc
     1261 caggtctcgc tgaagccggc ggggcggtcg tccacggcgg ggtagacgac gtgcgccggc
     1321 atcacgctgg cgagggaggt cgacagccat tcgtacgggc gcgcgtcgtc ggcgaggatc
     1381 tccgagagcc ggcgcgggtc gacggggatg tcgacgtggc tgtccgccgc gacgtggccg
     1441 tggccgggga agtgcttgcc gcagtgcgcc atgcccgcct ggcgcatgcc ggcggccagg
     1501 ctcttggcga gcagcgcgac cacgcgcggg tcgcggtgga aggcgcggtc gccgatgacc
     1561 gcgctgtggc cgaagtcgag gtccagcacc ggcgcgaagc tcaggtcgac gccgcaggcg
     1621 cgcagctcgg cggccagcac gaagccggcc gcggtcgcgg cgtcggtggc ggccatcgcg
     1681 ccgtcgccgg gcgcgccacc ggcgtcgtcc atccagcgcc ggccgtagtc ggccatcggc
     1741 ggcaggtgcg tgaagccgtc ggtgcggaag cgctgcacgc ggccgccctc gtggtcgacg
     1801 cacacgagca ggtccggccg gatcgccttc atctccgcga cgagctcggt cacctgtcgc
     1861 cggttcgtcc agttgcggcc gaacaggatc gcgccgccga ccagcggatg cgcgaggcgg
     1921 cggcgatcgg ccgcgtcgag cgtcgtgccg gcgacgtcga ggatcacggg tgcatggatg
     1981 cccggcgcgg ccgcctcggc gctgtcgtcg cggtcgtcgt cgcggtggtt cttcgtctgg
     2041 ggcttgtggt cggcacgggc catggcgtcg gtcaggcagt cgggagttgt cgggtctcga
     2101 ccaccacgaa ggtggccacg tagtcggtct cgtcggtgac ggtcacgtgc gcgacgaggc
     2161 cgcgcgcctc gaaccactcg gcgagcgcgc cgtgcagccg gatcaccggc ttgcccgagc
     2221 gctcgttgag gatctcgcag tcgcgccagg tcatcggcat gtgcaggccg aggccgatcg
     2281 ccttggagaa cgcctccttg gccgagaagc gcgtcgcgag gaagcgcagc ccgcggccct
     2341 cgacgcgcgc gcgccgggcc cggaacacct cgatctcgcc cggcccgagc accttctcgg
     2401 cgaagcgatc gcccttgcgc tcgagcgtgg ccgcgatgcg gcgcacgtcg cagaggtcgg
     2461 tgccgatgcc gtagacggtc acgggtcgat cgcctcaggc gagggagtcg gccggctcgg
     2521 cgtcggcgat cgcgcgcagg tagcggtgca ccgtctccgt catgccgaac tcgagcgcgt
     2581 cggccaccag cgcatggccg atcgagacct cggccaggcc cggcaccgcg cgcacgaagt
     2641 cggtcaggtt ggcgaggttg aggtcatgcc ccgcgttgac gcccagtccc gccgcgaggg
     2701 cggcctgcgc gctgcgcgcg aactgcgcga gcacctccgc gccccgcggc gtgccgtgcg
     2761 ctgccgcgta cggctcggta tagagctcca cgcggtcggc gccgaccgcg cgcgcgagcg
     2821 ccatcgcctc gggcaccgga tccatgaaca ggctcacgcg cacgcccagg ctgcgcgtct
     2881 cctcgatgat cgggcgcagc cgcgcggcgt ccctggccag atcccagccg tggtcggacg
     2941 tgaactggcc ttcgctgtcg ggcacgaagg tcgcctggtg cggtctcacc gcccggatga
     3001 agtccatcag gttgtggaac gggttgccct cgatgttgaa ctcggcctcg ggccagcggg
     3061 ccatcaacgc gtgcagctcg gggacgtcgg cggcgcggat gtggcgctcg tcgggccgcg
     3121 ggtggatcgt gatgccctgc gcgccggcct gcagcgcgag cgtggcggcg tgcaccacgc
     3181 tcggaatgcc gaggtggcgc gtgttgcgca gcagcgcgac cttgttgacg ttgaccgaca
     3241 gcgcgcagcg ctcgtgcgca cggcgggtct gttcgggggc gacgagcggg ttcatggcga
     3301 cttcctcgtg gatagcgcgt cagggcgcat cgatgagatg gcgcgcgtcg cgcatcacct
     3361 cgcgggtgcg cagcacgggc gagccgagat gatagtgaac cagcgtgcgc aacaccggcc
     3421 gcaggcgatc gagccgcgtc gcgcaggcca cgtgcagcgg gccgagcgac tcctgcgcca
     3481 gcgccgcctg cagcgcgagc aggtcggccc cgggcaggct cgcgaagccg agctgcggat
     3541 cggcctcgat gacgccgcga tcgcctagcc actggtacgc ggccgccggt gcgagcggcc
     3601 gctgcgtcgc cgtgacgcga tcgagctccg gcagcaggcc gatggcgcgc agcagcacga
     3661 actcgaacgc gcgcaggccg atctgctcgc cgaactcgtc ggccgtcgcg aggctgcgca
     3721 aggtcgccgc atacgcgtcg aacagggccg ggtgcgcgtc gccgcgggcg agcagcttca
     3781 tcagcagctc gttgaggtag aagccggcga acagccgcgc gggcggcagc accggcgtgg
     3841 cgtcgtctcc ggcccactcc gcgccgcgca gcgtgacgac gtcggcctgc gcctcctcgg
     3901 cctgcttgcc gaccgtggcc gcgatgcgct ggaacggcag cagcacgctg cgcaactgcg
     3961 agtacggccg cttcgcgccc ttggccgcga cggcgatgcg gccgcgttcg cgcgtgaaca
     4021 ggtcgaggat caggctggtc tcgctccagt cgtagcgatg gagcacgtag gcggccagcg
     4081 gcgccgcggc gcggcgagcg gtgggcatgg cgctaccgcg gcgcggcggc cggcgcgcgc
     4141 gggctactcg tagccatacg agcgcaggtg ctcttcggaa tcggcccagc cggagcgcac
     4201 cttcacccac acctccagga acaccttcga ctcgagcagc cgctcgagct cctggcgcgc
     4261 ctcggacgcg atgcgcttga gctgctggcc cttggcgccg atgatcatgc ccttgtgcgc
     4321 gtcgcgctcg accaccacgg tcgccgcgat gcgcttcatc gcgccttcct cgacgaactg
     4381 gtcgatcacg accgtcgcgc tgtacggcag ctcgtcgccg gtcaggcgga acagcttctc
     4441 gcggatcacc tcgctcacga ggaagcgctc gctgcggtcg gtgagcatgt ccgcgtcgta
     4501 gaaccacggc tgctcgggca ggtagggctg gatgacgccg gccaggcggt cgacgtcggc
     4561 cttcgaatgc gaggacagcg ggaagaactc gttgaacgga tggcgctgct gcatctgctg
     4621 cagccagggc agcaggtccg agcggcgctg cacggtgtcg agcttgttgg cgacgaggaa
     4681 ggccggcttg cccttgggca gcagctgcag cgccttcgcg tcatcgctgc cgaacctgcc
     4741 ggcctcgacc atgaacagca cgacgtcgac gtcggtcagc accgagacga ccgtcttgtt
     4801 cagcgacttg ttcagcgccg ccgagtgctt catctggtag cccggcgtgt ccacgaagac
     4861 gaactgcgcc ggcccgtcgg tgcgcacgcc gacgatgcgg tggcgcgtgg tctgggcctt
     4921 gcgcgaggtg atgctgatct tctggccgac cagcgcgttc atcagcgtcg acttgccgac
     4981 gttgggccgg ccgacgatcg cgaccaggcc gcagcgcttc ctcgcgccgt gctcgtcgac
     5041 ggcgtcggcg agcatcgcgg ccatcgcctc cgcgctcagc ggcgtctcga tcatgtcctc
     5101 gtcgccgtcg ccggcatggg cgtcgtcgtc gccctggtcg agatcgtcga tgccgtcggc
     5161 accctcgatg tcctcgtgcg gcggcacgtc gtcgtcggtc ggttccgggg actggggatc
     5221 aggcactggg cggtcctttg cgggcaggca cgtcgagctc gccggccttg atgcgatcga
     5281 gcaggtgctg cgccgacgcc tgctccgcgg cgcggcgcga gcggccttcg ccgcgcgcga
     5341 gccacttcag cgtcggcacc tcgcactcga cctcgaaggt ctgcgcgtgc gcctggccgt
     5401 gggtggcggt gatgcggtag ctcggcacgg gcacgtggcg cgcctgcagc cattcctgca
     5461 gctcggtctt ggcgtccttg cgccagttgt cggcggtcgt cgcgttgatc gtctccccga
     5521 acagccgcgc caccagcgcc tgcgccgcgt cgaagccgcc gtcgatgctc accgcgccga
     5581 tcagcgcctc gaccgcgtcg gcgaggatcg acggccgcgt cgcgccgccg ccgcgggcct
     5641 cgccctccga caggcgcagc gcgtccggga gcccgagcgc gagcgcgacg cggtgcaggc
     5701 tgtcctcgcg cacgaggtgg gcgcgcacgc gcgtgaggtc gccctcgtcg gagccggaga
     5761 agcggtcgaa cagcagccgc gagaccgcga ggttgagcac cgcgtcgccg aggaactcga
     5821 ggcgctcgtt gtggtcggca ccgaagctgc ggtgggtcag cgcacgctcg aacagggcgc
     5881 gctgggcgaa ttcgtagccg aggcgcttct cgaccatcgc cgcgccgcgc gcggccggag
     5941 acggcgtcgc cctggtcatg cgtggctcag acgttgggcc cgccgccggc cgcggacgcc
     6001 tcgcccgagt acttgatcag gatgaacacg ggctcgacga cgggaatctc gacgttgtac
     6061 gcgaacgagc agcgcaggtg gtcgcccacc tgctggatgt cgaggtcctt gccggagatc
     6121 gaggtgatcg agtactcgac gtccttcgcg cgatcgaagg acttgcggat ctccgccggg
     6181 ttcgtcgggc tgtcgtgcat gatctggcgg acgaccttgc gcacggtgag gaactcgttg
     6241 accgacggga acacgcgcat cgccatgagg ccgatgaagc agacgatcac cgcgatcacg
     6301 aacaggccga acagggacgt gcctcgctgc ggggcgtgga ggcggcgacg ggtcggatgc
     6361 atggcggggc cttcggtcgg tcgacggtcg atggagggga ggcgcggggc gacgctcagt
     6421 ggaacgagcc gattctgctc ggatcgccga agttcatcca gacgaagaac gcacggccga
     6481 cgatgttctc gtcgggcacg aagccccagt agcgggagtc ctcggagtcg tcgcggtggt
     6541 cgcccatcat gaagtaatgg cccggcggca ccttgcacac gacgccctcg gcgttgtagc
     6601 ggcagttgtc cttgaacgcg aagctgttga acggctggac gtacgacggc cggtcggagc
     6661 gcgtgaggat gcggtgcttc acgccgtcca ggtcctcctc gaaggcctgc tcgtagacgg
     6721 cgctgctctc gacgaagtag tccggcagcg gcgtctcggt gatgagcttg ccgttgatgc
     6781 gcagctgctt gttcaggtag gagacctcgt cgccgggcag gccgacgacg cgcttgatgt
     6841 agtccacctc cggatggatc gggtagtgga agaccatcac gtcgccgcgc tgcgggtcgt
     6901 ggttcgggat gatcttcttg ttgatcaccg gcaggcggat gccgtagtgg aacttgttga
     6961 ccaggatcag gtcgccgatc tgcagcgtcg ggatcatcga gcccgagggg atcttgaacg
     7021 gctcgaacag gaagctgcgc aggacgaaga cgacgacgat gaccgggaac aggccggccg
     7081 tccagtcgag ccaccacggc tgcatcagca ggcgcgcctt ggcctcctgc aggtcgccgt
     7141 cgaccttgtc gatccccatc cgggcaagct cggcacggcg ggcgacgtcc tgggcctcga
     7201 gcacggcggc ggccttctcg cgcttcgggc ggaagtacaa ccgctcggcc agccagtaca
     7261 ggaaggtcac ggtcgtcagc atcagcagca gcaacgcgaa gttgccgggc caccagtcct
     7321 tcagccagcc gccgagatag atcaccaggg cggcatacag cgcgccggtc aatgcactca
     7381 tcgattcagt cgtccacttg gaggatggcg aggaaagctt cctgaggcac ttcgacggag
     7441 ccgatctgct tcatccgctt cttgcccgcc ttttgttttt cgagcagctt gcgcttgcgg
     7501 gtgatgtcac cgccgtagca ttttgccagc acgttcttgc gcagcgcctt gatgttctcg
     7561 cgcgcgatga tgttggcgcc gatcgcggcc tggatcgcga cgtcgtacat ctggcgcggg
     7621 atctgctcgc gcatcttggc ggccacggcg cggccgcgga actggctctg ggagcggtgg
     7681 acgatcatcg ccagcggatc gaccttgtcg ccgttgatca ggatgtcgac cttcaccacg
     7741 tcggccgagc ggtactcctt gaactcgtag tccatcgacg cgtagccgcg cgagacggac
     7801 ttcagcttgt cgaagaagtc cagcacgatc tcggccagcg gcagctcgta ggtcagcatg
     7861 acctgcttgc cgtggtaggc catgttcagc tgcacgccgc gcttctggtt gcacagcgtc
     7921 atcacgacgc cgacgtagtc ctgcggcatg tagaggtgca cggtgacgat gggctcgcgg
     7981 atctcgcgga tgcggccctg gtcgggcatc ttgctcgggt tctcgacctc gagcaccgtg
     8041 ccgtcgttga gctcgacctc gtagaccacg ctgggagcgg tcgtgaccag gtcctggtcg
     8101 aactcgcgct cgaggcgctc ctgcacgatc tccatgtgca gcaggccgag gaagccgcag
     8161 cggaagccga agcccagcgc ctgcgagacc tcgggctcga agcgcagcga gctgtcgttg
     8221 agcttcaact tctccagcgc gtcgcgcagc tggtcgtact cgctcgcctc ggtcgggtac
     8281 aggcccgcga agacctgggg ctggatctcc ttgaagccgg gcagcggcgt gtcggcgggc
     8341 ccggcgttgt tcggcagctt cttctcgagc gtcagcgtgt cgccgacctt ggcggccgcg
     8401 agctccttga tgccgcagat cacgaagcca acctcgccgg cgttcagcgc gtcgcggttc
     8461 tcgctcttgg gggtgaagac gcccaggtgc tcgatgccgt agaccgtgtt ggtcgccatc
     8521 aggcggatgc gctcgccctt ggcgaagcgg ccgtcgacca cgcgcaccag catcacgacg
     8581 ccgacgtagt tgtcgaacca cgcgtcgatg atcatggcgc gcggcggccc gtccgggttg
     8641 cccctcggcg gcggcatgcg ggcgatgacc gcctcgagga tgtcgtcgat gcccatcccg
     8701 gtcttggcgg agcacgggat cgcgtgctcg gcgtcgatgc cgatgacgtc ctcgatctcg
     8761 gccttcgcgt tgtcgggatc ggccgagggc aggtccatct tgttcaggac cggcaccacc
     8821 tcgacgccca ggtcgagcgc cgtgtagcag ttcgcgaccg tctgcgcctc gacgccctgg
     8881 ctcgcgtcga cgaccagcag cgcaccctcg caggcggaca gcgagcggct gacctcgtac
     8941 gagaagtcga cgtggcccgg ggtgtcgatg aggttgaggt tgtagacctg gccgtcgcgc
     9001 gcctggtatt gcagggccgc ggtctgcgcc ttgatcgtga tgccgcgctc gcgctcgatg
     9061 tccatcgaat ccaggacctg cgcttccatc tcgcggtccg acaggccgcc gcagcgctgg
     9121 atcaggcggt ccgccagcgt gctcttgccg tggtcgatgt gggcaatgat cgagaagttt
     9181 ctgatgtgat ccatggagag tcgaactgac ggcgaacgag cgcgccggtc gacgcgggag
     9241 gccgcttcgg cgcggcccgt cgcaggcggc tgcggacggg cgtttcaggt ggcgggacgc
     9301 cgggcgccgg gactaaaaaa aaggcacgtc caagagaaga cgcgccttcc aacaaaacgt
     9361 atggcaactg cttgttgggc cttcattgta gtcaaaaggc cgccggtgga aaccgcgtct
     9421 ttgctgggtt tttacggcgt tgcaggcccc caacacgaaa attcatccac agcttatgca
     9481 caggctttgg accctgcgtc gcgaacgggc gggcgcttgc gaagtcggcc cggaagaccg
     9541 caccgaccca gcgatttcgg cccgaattgt accgtttcgc ggcacccggc tgtggacagt
     9601 cggaatgaag tttgtacgca ctgacatggc gccccgacct gtccgaacgg atcgccgggg
     9661 cccactcgtt cgccatgcgc acatggctcc tgccaggtga aaagacccgt cggcccactc
     9721 ggctgtcggg tcattgccgc cgggctggcg cggctggcag gaatcgaacc tgcgaccctc
     9781 tccttaggag ggagatgctc tatccactga gctacagccg cgcgaggcgc gcagtgtagc
     9841 aagaggccct cccgccggac ggcgcgccga ttgccgcaat gcctccgggt tccgtgcgga
     9901 atgcacggtg ttccggcctc gttttcccgc ttgcgagcgc ccgctctaca ggaaagcgcg
     9961 tcctatactt gtgcaatgcg cgcgtgccga cggcaggggc cgcaccccgt gcgcaaggat
    10021 tcgaggcgtt caaagacatg ggttcgaaat tcaaggttgc ggggctgctg gccctcgggg
    10081 ccgtcgccgg tgcgctgacg acgatgcaga tccaggcgat cgcccgcaac acgctgggct
    10141 cgttcggctc gctgccgctg gacgacatcg agcgcttcac ggaagtgttc gaccgcgtca
    10201 agacgaacta cgtggtcccg gtcgacgaga agaagctgat cggcgacgcc atcggcggca
    10261 tggtctccgg cctcgacccg cactcgcagt acttcgacaa gaagtcgtac aaggaattcc
    10321 aggagtccac gtcgggccgc ttcgtgggcg tgggcatcga gatcggcatg gaggacggac
    10381 tcgtcaagat cgtcacgccg atcgagggct cgccggcctt ccgcgccggc ctgaagtccg
    10441 gcgacctgat cacgcgcatc gacgagacgc aggtcaaggg cctcacgatg gaccaggccg
    10501 tcaagaagat gcgcggcgac ccgggctcca aggtcgtcct gacgatcttc cgcaaggacg
    10561 agggccgcac gttcccggtg acgatcgcgc gcgaggagat ccacgtccag agcgtcaagg
    10621 ccaaggactt cgacggctac gcctgggtgc gcatcgcgca gttccaggag cgcaccgtcg
    10681 acgacttcgc ggccaaggtc gaggacctgt acaagaagga cccgaagatc aagggcttcg
    10741 tgctggacct gcgcaacgac ccgggcggcc tgctcgatgg cgcgatcggc atcgcgtcgg
    10801 tcttcctgcc gcccgacagc gtcatcgtga agaccaatgg ccagatgccg gactcgaagc
    10861 agaccttcaa ggcctcgccc gaggattacc gccgctccgg cgccgacccg ctgcgcaagc
    10921 tgccggccgc gctcaagacg gtgccgctgg tggtgctgat caacgaaggc tcggcctcgg
    10981 ccagcgagat cgtcgccggc gcgctgcagg actacaagcg cggcaccatc atgggcgcgc
    11041 agtccttcgg caagggctcg gtgcagacgg tgctgcgcct gcagggcgac accgcgatca
    11101 agatcacgac cgcgcgctac tacacgccca gcggccgctc gatccaggag aagggcatcg
    11161 tgccggaaat ctggctcgac gagaccgccg aaggcaacct ctattcggcg ctgttcccgc
    11221 gcgaggaaga cctggacaag cacctcgtca acgacgagta caaggccgat cccgccaagg
    11281 agaaggcccg cgaggcggcg cgcgaggcgg ccgaggccaa gctcgccgag gagcgcgcga
    11341 agaacggcaa caagccgatc aagccgctgc cggacttcgg ctccgccgag gacttccagc
    11401 tgaaccaggc gctcaacgag ctgcgcggcc agccggtcgt cgtcagcaag acggccaccg
    11461 agcgcaagcc cgaggacacc tccacccagt gaccgcggcg ccggccgcgc gtccgcacga
    11521 cgccgccgac ggccctgccg cggcggcgtt cttcatggtg caccgacgac cgacgtgacc
    11581 gacctgaccg acgacgacct gctgcgctac tcgcgccaca tcctgctcga cgagatcggc
    11641 atcgagggac agcgccgcat cgccgccgcg cgggcgctcg tgatcggcgc gggcggactg
    11701 ggctcgcccg ccgcgctcta cctgggcagc gcgggcatcg ggcggctcgt gctcgtcgac
    11761 gacgacgcgg tcgacctcac caacctgcag cgccagctcg cgcataccca ggaccgtatc
    11821 ggaacgaaca aggcgcgctc ggcgcgcgcc gcggtgctcg cgctgaaccc ggggctggac
    11881 gtggttgcgc tcgagcgccg cgcgggtgtc gcggacctcg cggcgctcgc cgccgaggcc
    11941 gacgtcgtgc tcgattgcag cgacaacttc gccacccgcc aggcggtcaa cgcggcgtgc
    12001 gtcgccgcgg gcgtgccgct tgtcgcgggc gcggcgatcg gcttcgacgg ccaggtctc
//
LOCUS       DASVLN010000659         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_195031, whole genome shotgun sequence.
ACCESSION   DASVLN010000659 DASVLN010000000
VERSION     DASVLN010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195031"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VF453_15575"
     CDS             complement(<1..607)
                     /locus_tag="VF453_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689134.1"
                     /translation="MNLPTPALVTRRAAQRLPRWALILLCAAYLLPGQFGREPWKNAD
                     ITAFGYMASIAEGRSPWTAPAIASLPGDGALLPYWLGALAIKAMPFLDPALAARLPFA
                     ALLAATLAFVWYSTQHLARTEAAQPAAFAFGGEASPTDYARALADGSVLALIATLGLL
                     QLGHETTPELVQLSGVSLFMWVLSAAPTRPRAAAACAVAALA"
     gene            complement(754..1014)
                     /locus_tag="VF453_15580"
     CDS             complement(754..1014)
                     /locus_tag="VF453_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEX7689135.1"
                     /translation="MKDGIHPNYRDVCFVDLSNGFKFVTRSCAQTKETIKMDDGKELP
                     LFKLETTSESHPFYTGTQKSVDNLGGRVEKFRNKFARVGLKK"
     gene            complement(1236..2498)
                     /gene="rho"
                     /locus_tag="VF453_15585"
     CDS             complement(1236..2498)
                     /gene="rho"
                     /locus_tag="VF453_15585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX7689136.1"
                     /translation="MHLSELKALHIGALITMGEALEIDNVARMRKQELMFAIMKKRAK
                     GGEQVFGDGVLEVLPDGFGFLRAIDESYMASTDDIYLSPSQIRRFNLHTGDMIEGEVR
                     VPKDGERYFALVKVDRVNGVSPEESKHKIMFENLTPLFPKEVFRLERDMRGDENVTGR
                     IIDLIAPIGKGQRALLVAQPKTGKTVMMQHIAHALVANHPDIHLIVLLVDERPEEVTE
                     MLRTVRGEVISSTFDEPAQRHVQVAEMVIERAKRLVEMKKDVVILLDSITRLARAYNN
                     VLPSSGKVLTGGVDANALQRPKRFFGAARNVEEGGSLTIIGTALIDTGSKMDEVIYEE
                     FKGTGNCEIHLDRRMAEKRVYPSIQINRSGTRREEMLLKPEILQKTWILRKLLYPMDE
                     IEAMEFMLDKMKASKTNLDFFDMMRRGG"
     gene            complement(2895..3236)
                     /gene="trxA"
                     /locus_tag="VF453_15590"
     CDS             complement(2895..3236)
                     /gene="trxA"
                     /locus_tag="VF453_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEX7689137.1"
                     /translation="MSSAAIKHISDASFEADVLKSGTPVLVDYWAEWCGPCKQIAPIL
                     DEVAKDYDGRLQIAKLNVDDNRDIPAKFGIRGIPTLMLFKDGQLAATKVGALSKSQLL
                     SFLEGHLPATA"
     gene            3547..>4443
                     /locus_tag="VF453_15595"
     CDS             3547..>4443
                     /locus_tag="VF453_15595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7689138.1"
                     /translation="MAVQAPPTAATAAPNRAVRMLGRYQLLRLLGKSAQTMVWLGQDT
                     QGESEVVLALPRVQPPDPDALGAWLARVRRSGRLKHPRLAPVLDVGEYERWPYAVYER
                     GAFATWSERLGGQPLPPQELAQWCVQVLQGLAFAHEAGVSHRDLQPHMLQVDDGGNVR
                     LLGLDIADSHSMAQLASDAGLGHEAAMDERRIQRQSAEIDVLALGLLMHQALAGAPPL
                     DEPDVARVVGRLPPFGHEMVRLPWTVPMPVPEPLRAIVNRATDRQERQRYRNARTFER
                     ALEGWLQASGDKDGGPITLLLGR"
BASE COUNT          653 a         1479 c         1605 g          706 t
ORIGIN      
        1 ccgcgagcgc cgcgaccgcg caggcggcgg ccgcgcgcgg tcgcgtcggc gccgcggaca
       61 gcacccacat gaacagcgag acgccgctga gctgcacgag ctcgggcgtc gtctcgtggc
      121 ccagttgcag caggcccagc gtggcgatca gggcgagcac cgagccgtcg gcgagcgcgc
      181 gcgcgtagtc ggtcggcgaa gcctcgcctc cgaaggcgaa ggcggcgggc tgcgcggcct
      241 ccgtgcgcgc gaggtgctgc gtgctgtacc agacgaaggc gagcgtggcc gccagcagcg
      301 ccgcgaaagg cagccgcgcc gcgagggccg ggtcgaggaa gggcatcgcc ttgatcgcga
      361 gcgcgccgag ccagtagggc agcagggcgc cgtcgccggg caggctcgcg atggcggggg
      421 cggtccacgg gctgcggccc tcggcgatcg aggccatgta gccgaaggcg gtgatgtcgg
      481 cgttcttcca gggctcgcgg ccgaactgcc cgggcagcag ataggcggcg cacagcagga
      541 tcagcgccca gcggggcagg cgctgggccg cgcgccgggt gacgagtgca ggggttggca
      601 gattcacggg gggcatcatg ccgcaagcgc gggccgcggc cggagcggat ggcgaaaaag
      661 acgcggacac aaaaaaggca gcccgaggct gcctttcgtg gttccagaga atccggatcg
      721 ccgcggcgcg agggccgcgg acggacgtcg cgcttacttc ttgaggccga cgcgggcgaa
      781 cttgttgcgg aacttctcga cgcggccgcc caggttgtcg acgctcttct gcgtgcccgt
      841 gtagaacggg tgcgactcgc tggtggtctc gagcttgaac agcggcagct ccttgccgtc
      901 gtccatcttg atcgtttcct tggtctgcgc gcacgagcgc gtcacgaact tgaaaccgtt
      961 ggacaggtcg acgaagcaga cgtcgcggta gttggggtgg atgccgtctt tcatgggatg
     1021 ccctcgatct cgctttgccg ggaacgacgg gcctgggcga aggactcgcg cagtgccgcc
     1081 ggcgtcgtcg gaagccactg cgcaccatgc acagcacttt ccgaaagcgg aaaaccttgg
     1141 attctaaacc atgatgcgtc gcgctgtcca ggcgggccac cgaaacgaaa aaggcccgcc
     1201 gaagcgagcc tttccgacag acgagccagg gcgggtcagc cgccgcgccg catcatgtcg
     1261 aagaagtcca ggtttgtctt gctggccttc atcttgtcga gcatgaactc catcgcctcg
     1321 atctcgtcca tcgggtagag cagcttgcgc aggatccagg tcttctgcag gatctccggc
     1381 ttcagcagca tctcctcgcg gcgcgtgccg ctgcggttga tctggatcga cgggtagacg
     1441 cgcttctcgg ccatgcggcg atccaggtgg atctcgcagt tgccggtgcc cttgaattcc
     1501 tcgtagatga cttcgtccat cttcgagccg gtgtcgatca gcgccgtgcc gatgatggtg
     1561 agggatccgc cttcctcgac gttgcgcgcc gcgccgaaga agcgcttcgg gcgctgcagc
     1621 gcgttggcgt cgacgccgcc cgtgagcacc ttgcccgacg agggcaggac gttgttgtag
     1681 gcgcgggcca ggcgcgtgat cgagtcgagc aggatcacga cgtccttctt catctcgacc
     1741 aggcgcttgg cgcgctcgat caccatctcg gcgacctgca cgtggcgctg ggccggttcg
     1801 tcgaaggtgg agctgatcac ctcgccgcgc acggtgcgca gcatctcggt gacttcctcg
     1861 gggcgctcgt cgacgagcag cacgatgagg tggatgtccg gatggttggc gaccagcgcg
     1921 tgggcgatgt gctgcatcat caccgtcttg ccggtcttgg gctgggcgac gagcagggcg
     1981 cgctggcctt tgccgatcgg cgcgatcagg tcgatgatgc ggcccgtcac gttctcgtcg
     2041 ccgcgcatgt cgcgctcgag cctgaacacc tccttcggga agagcggcgt caggttctcg
     2101 aacatgatct tgtgcttgct ctcctcgggc gagacgccgt tgacgcggtc gaccttgacc
     2161 agcgcgaagt agcgctcgcc gtccttgggc acgcggacct cgccctcgat catgtcgccg
     2221 gtgtgcaggt tgaagcggcg gatctggctc gggctgaggt agatgtcgtc ggtcgacgcc
     2281 atgtagctct cgtcgatcgc gcgcaggaag ccgaagccgt ccggcagcac ttcgagcacg
     2341 ccgtcgccga acacctgttc gccgcccttc gcgcgcttct tcatgatcgc gaacatgagc
     2401 tcctgcttgc gcatgcgagc cacgttgtcg atctcgagcg cttcgcccat ggtgatcaga
     2461 gcaccgatgt gcagggcctt gagttcggaa agatgcattg ttcgttcacc ctggggtggc
     2521 ggccgcagcc gattccttca gccgcgcggg gattccggcg cggtgtgcgg tcgcgagcgg
     2581 ccaccgatcc gattcctcgt gcacgaccca ggggtcggcg cggcaggatg ccggatcagc
     2641 gccgtcgcga cggcgtggaa cggtagggct ttgaaatttc gccgaaaccg cgcgccgggt
     2701 cccggtcggg agcagggcgc gggggcgggg ccttcgatcg gtggggagac gtgcccttca
     2761 gcagagcggg tcgcccggaa tcaccgacgt ggcgggatta tagggggccg tgcagcaacg
     2821 gcgtcaactg ccgtttcagg ccgcgcgtcg gccggatggg gtcggcgacg cgccggccgc
     2881 ctccggccgg ggcctcaggc cgtcgccggc aggtggcctt cgaggaagga cagcagctgc
     2941 gacttcgaca gcgcgccgac cttggtcgcg gcaagctggc cgtccttgaa cagcatcagc
     3001 gtcgggatgc cgcggatgcc gaacttggcc gggatgtcgc ggttgtcgtc gacgttgagc
     3061 ttggcgatct gcaggcggcc gtcgtagtcc ttggcgacct cgtccaggat cggcgcgatc
     3121 tgcttgcagg ggccgcacca ttccgcccag tagtcgacca gcaccggggt gccggacttc
     3181 aggacgtcgg cctcgaagga cgcgtcggag atgtgtttga tagctgcgct gctcatgcgg
     3241 attccttggc tttccctcgc gtccggcggc acgggccggg ggaggacgtc tcgggtcgaa
     3301 agattctgac acatccgtcc agacccggcc gccgtcaagg atgcccctgg cggcacgctt
     3361 cccggacggg acgccgggcc ggcggcggct cgcccgccgg cccgactcga caaatggggc
     3421 cggcggccga aaagagcaag ggcgaccggc tgaaatgcga aatgcgcatc gaaacgggcg
     3481 ctcttccgcg cgccgcgtcg gccggcaccg cgttcaaatc gagcgatcct cccgcactcc
     3541 gtgcccatgg ccgtccaagc tcctcccacc gccgccacgg ccgcgcccaa ccgcgcggtg
     3601 cgcatgctcg gccgctacca gctgctgcgc ctgctcggca agagcgcgca gacgatggtc
     3661 tggctgggcc aggacaccca gggcgagtcc gaggtggtgc tggcgctgcc gcgcgtgcag
     3721 ccgccggatc cggacgcgct gggcgcctgg ctcgcgcgcg tgcgccgcag cggccggctc
     3781 aagcatccgc ggctggcccc ggtgctcgac gtcggcgagt acgagcgctg gccctacgcc
     3841 gtctacgagc gcggcgcgtt cgccacgtgg agcgagcgcc tcggcggcca gccgctgccg
     3901 ccgcaggaac ttgcgcagtg gtgcgtccag gtgctgcagg gcctcgcgtt cgcgcacgag
     3961 gccggcgtct cgcaccgcga cctgcagccg cacatgctgc aggtcgacga tggcggcaac
     4021 gtgcgcctgc tggggctgga catcgccgat tcgcactcga tggcacagct ggcgagcgac
     4081 gccggcctcg gccacgaggc ggcgatggac gagcgccgca tccagcgcca gtccgccgag
     4141 atcgacgtgc tcgccctcgg cctgctgatg caccaggcgc tggccggcgc gccgccgctg
     4201 gacgagcccg acgtcgcgcg cgtcgtcggc cggctgccgc ccttcggcca cgagatggtg
     4261 cggctgccct ggacggtgcc gatgccggtg cccgagccgc tgcgcgcgat cgtcaaccgc
     4321 gccaccgacc gccaggaacg ccagcgctac cgcaatgcgc gcaccttcga gcgcgcgctc
     4381 gagggctggc tgcaggcgag cggcgacaag gacggcggcc cgatcacgct gctgctcggg
     4441 cgc
//
LOCUS       DASVLN010000660         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_519365, whole genome shotgun sequence.
ACCESSION   DASVLN010000660 DASVLN010000000
VERSION     DASVLN010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519365"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VF453_15600"
     CDS             <1..140
                     /locus_tag="VF453_15600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX7689139.1"
                     /translation="PYRLKIRAPGFAHLAAMDEMSRGHMLADAVAVIGTMDIVFGEID
                     R"
     gene            137..655
                     /locus_tag="VF453_15605"
     CDS             137..655
                     /locus_tag="VF453_15605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX7689140.1"
                     /translation="MTQHTDNTAAHVPFTDEMRAQFAREVAKFPAAHAQSAVMACLSI
                     IQHERGWVSRAAEDELAEYLGMAPIAVYEVTTFYNMYNQAPVGKFKINVCTNLPCQLR
                     DGQAALAHVCKKLGVTVGGTTADGQFTVQQSECLGACADAPVMLVNDREMISFMSNER
                     IDDLLATLAAAK"
     gene            675..2018
                     /gene="nuoF"
                     /locus_tag="VF453_15610"
     CDS             675..2018
                     /gene="nuoF"
                     /locus_tag="VF453_15610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX7689141.1"
                     /translation="MVDLSRFKAQGVETAFHDRHLNPQILAGLDGSNWRLKDYEARGG
                     YQALRKILAQDGTGMTPDQVVAEVKASGLRGRGGAGFPTGLKWSFMPRQFPGQKYLVC
                     NSDEGEPGTCKDRDILAYNPHIVIEGMAIAAYAMGISVGYNYIHGEIFEIYQRFEEAL
                     EEARAAGYLGDGILGSKFNFQLHGFHGFGAYICGEETALLESLEGKKGQPRFKPPFPA
                     SFGIYGKPTTINNTETFAAVPWIIRNGGQAYLECGKPNNGGTKIFSVVGDVNKPGNYE
                     IPMGTPFSTLLELAGGVRTGRKLKAVIPGGSSAPVLPASIMMELTMDYDSIAKAGSML
                     GSGAVIVMDDSRCMVESLLRLSYFYQHESCGQCTPCREGTGWLWRMVDRIEHGHGRME
                     DIELLNSVADNIQGRTICALGDAAAMPVRAMIKHFRSEFVHHIEHKTCVVPATTA"
     gene            2046..>4057
                     /gene="nuoG"
                     /locus_tag="VF453_15615"
     CDS             2046..>4057
                     /gene="nuoG"
                     /locus_tag="VF453_15615"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX7689142.1"
                     /translation="MIEIELDGKKVSVPEGSMVMHAADAADTYIPHFCYHKKLSIAAN
                     CRMCLVDIEKAPKPMPACATPVTQGMIVRTKSDKAIKAQQSVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGGSASRYTEEKRVVFHKPAGPLLSMEEMSRCIHCTRCVRFGQEV
                     AGVMELGMAHRGEHSEIQTFVGRSVDSELSGNMIDICPVGAITSKPFRYQARTWELSR
                     RRSVSPHDSVGANIVVQVKNNQVLRVVPFENEDVNECWIADRDRFSYEALNHESRLTA
                     PMIKQNGQWQQVDWPTALGYVADGVKRVKAEFGVAGIGALAHPNSTVEEMHLLAKLVR
                     GLGSESVDHRLRHADFSNVGGVRWLGTSIASLSKLDRALVVGSFLRKDHPLLAQRLRQ
                     AARQGAQVFAVGANADDWLLPVAGRITAAPGAWVKALADIAHCVATVQGKAAPFAGET
                     TPEAQAIAASLIIGERGAVLLGNAAAQHPQAYTLLALANWIAQATGCTVGYLGDAGNN
                     VGAQLVNALPGPGGLNAGQMLSQPMRALFLLGVEPELDAADAAAARAALGASGLVVAL
                     TPFRNAAADVADVLLPISPFTETAGTFVNAEGRVQSFQGTCRPLGETRPGWKVLRVLG
                     DLLGLPGFGQQSSEEVLAEALGDRATLAARLDNRAALPSTLPADGAA"
BASE COUNT          678 a         1456 c         1348 g          575 t
ORIGIN      
        1 agccgtaccg gctgaagatc cgcgcgcccg gctttgccca cctggccgcg atggacgaga
       61 tgtcgcgcgg ccacatgctg gccgatgcgg tcgccgtgat cggcaccatg gacatcgtgt
      121 ttggcgagat cgatcgatga ctcagcacac cgacaacact gcggcgcacg tgccgttcac
      181 cgacgagatg cgcgcccagt tcgcgcgcga ggtcgccaag ttcccggccg cgcacgcgca
      241 gtccgcggtc atggcctgcc tgtcgatcat ccagcacgag cgcggctggg tctcgcgcgc
      301 ggccgaggac gagctcgccg agtacctggg catggcgccg attgccgtgt acgaggtgac
      361 cacgttctac aacatgtaca accaggcacc ggtcggcaag ttcaagatca acgtctgcac
      421 caacctgccg tgccagctgc gcgacggcca ggccgcactg gcccacgtct gcaagaagct
      481 cggcgtcacc gtcggcggca cgaccgcgga cggccagttc accgtgcagc agagcgaatg
      541 cctcggcgcc tgcgccgacg cgccggtcat gctggtcaac gaccgcgaga tgatcagctt
      601 catgagcaac gagcgcatcg acgacctgct cgccacgctg gcggccgcga agtaagaagg
      661 cgacggaaat ccaaatggtc gatctttccc gattcaaggc gcagggcgtc gagaccgcgt
      721 tccacgaccg ccacctcaac ccgcagatcc tggcgggcct ggacggcagc aactggcgcc
      781 tgaaggacta cgaggcgcgc ggcggctacc aagcgctgcg caagatcctg gcgcaggacg
      841 gcacgggcat gacgccggac caggtcgtag ccgaagtcaa ggcctccggc ctgcgcggcc
      901 gcggcggcgc cggcttcccg accggcctga agtggagctt catgccgcgc cagttcccgg
      961 ggcagaagta cctcgtctgc aactccgacg aaggcgagcc gggcacctgc aaggatcgcg
     1021 acatcctcgc gtacaacccg cacatcgtga tcgaggggat ggccatcgcc gcctacgcga
     1081 tgggcatctc ggtcggctac aactacatcc acggcgagat cttcgagatc taccagcgct
     1141 tcgaggaagc cctggaggag gcgcgcgccg ccggctacct cggcgacggc atcctcggct
     1201 cgaagttcaa cttccagctg cacggcttcc acggcttcgg cgcgtacatc tgcggcgagg
     1261 agacggcgct gctcgagtcg ctcgagggca agaagggcca gccgcgcttc aagccgccgt
     1321 tcccggcgag cttcggcatc tacggcaagc ccaccaccat caacaacacc gagaccttcg
     1381 cggcggtgcc ctggatcatc cgcaacggcg gccaggccta cctcgagtgc ggcaagccga
     1441 acaacggcgg cacgaagatc ttctcggtcg tcggcgacgt caacaagccg ggcaactacg
     1501 agatcccgat gggcacgccg ttctcgacgc tgctcgagct ggccggcggc gtgcgcaccg
     1561 gccgcaagct gaaggcggtg atcccgggcg gctcctcggc gccggtgctg ccggcctcga
     1621 tcatgatgga gctcacgatg gactacgact ccatcgcgaa ggccggctcg atgctgggct
     1681 cgggggccgt catcgtgatg gacgactcgc gctgcatggt ggagagcctg ctgcgcctgt
     1741 cctacttcta ccagcacgaa tcctgcggcc agtgcacgcc ctgccgcgag ggcaccggct
     1801 ggttgtggcg gatggtcgac cgcatcgagc acggccacgg ccgcatggag gacatcgagc
     1861 ttctgaactc ggtggccgac aacatccagg gccgcaccat ctgcgcgctc ggcgacgcgg
     1921 ccgcgatgcc cgtgcgcgcc atgatcaagc acttccgcag cgagttcgtg caccacatcg
     1981 aacacaagac ctgcgtcgtt cccgccacga cggcctgacc cggatagagc ggacgaaagc
     2041 gccccatgat tgaaatcgaa ctcgacggca agaaggtctc ggtgcccgaa ggcagcatgg
     2101 tgatgcatgc ggccgacgcc gcggacacct acatcccgca cttctgctat cacaagaagc
     2161 tctcgatcgc cgccaactgc cgcatgtgcc tggtggacat cgagaaggcg cccaagccga
     2221 tgccggcctg cgccacgccc gtcacgcagg gcatgatcgt ccgcacgaag agcgacaagg
     2281 cgatcaaggc gcagcagagc gtcatggagt tcctgctgat caaccacccg ctcgactgcc
     2341 cgatctgcga ccagggcggc gagtgccagc tgcaggacct cgcggtgggc tacggcggct
     2401 cggcctcgcg ctacacggaa gaaaagcgcg tcgtcttcca caagccggcc ggcccgctgc
     2461 tgtcgatgga ggagatgagc cgttgcatcc actgcacgcg ctgcgtccgc ttcggccagg
     2521 aagtcgccgg cgtgatggag ctgggcatgg cgcaccgcgg cgagcactcc gagatccaga
     2581 ccttcgtcgg ccgctcggtg gactccgaac tctcgggcaa catgatcgac atctgcccgg
     2641 tcggcgcgat caccagcaag ccgttccgct accaggcgcg cacctgggag ctctcgcgcc
     2701 gccgcagcgt gagcccgcac gactcggtag gcgccaacat cgtcgtccag gtcaagaaca
     2761 accaggtgct gcgcgtcgtc ccgttcgaga acgaggacgt caacgagtgc tggatcgccg
     2821 accgcgaccg cttctcgtac gaggcgctga accacgagtc gcgcctgacc gcgccgatga
     2881 tcaagcagaa cggccagtgg cagcaggtcg attggccgac cgcgctgggc tacgtcgccg
     2941 acggcgtcaa gcgcgtgaag gccgagttcg gcgtcgcggg catcggcgcg ctggcgcacc
     3001 cgaactcgac ggtcgaggag atgcacctcc tggccaagct cgtgcgcggc ctcggcagcg
     3061 agagcgtcga ccatcggctg cgccacgccg acttctccaa cgtcggcggc gtgcgctggc
     3121 tcggcacctc gatcgcgtcg ctgtccaagc tggatcgcgc gctggtcgtc ggctcgttcc
     3181 tgcgcaagga ccacccgctg ctcgcgcaac gcctgcgcca ggcggcgcgc cagggtgcgc
     3241 aggtcttcgc cgtcggcgcc aacgccgacg actggctgct gccggtcgcc ggccgcatca
     3301 ccgcggcgcc tggtgcgtgg gtgaaggcgc tcgccgacat cgcgcactgc gtcgccacgg
     3361 tccagggcaa ggccgcgccg ttcgccggcg agaccacgcc cgaggcgcag gccatcgcgg
     3421 cctcgctgat catcggcgag cgcggcgccg tgctgctggg caacgccgcc gcgcagcatc
     3481 cgcaggccta cacgttgctc gcgctcgcca actggatcgc gcaggcgacc ggctgcaccg
     3541 tcggctacct gggcgacgcc ggcaacaacg tcggcgcgca gctcgtgaat gcgttgcccg
     3601 gccccggcgg cctgaacgcc ggccagatgc tgtcgcagcc gatgcgcgcc ctgttcctgc
     3661 tgggcgtcga gcccgagctg gacgccgccg acgccgcggc cgcccgtgcc gcgctgggcg
     3721 cctccggcct cgtcgtcgcg ctgactccgt tccgcaacgc cgccgccgac gtcgccgacg
     3781 tgctgctgcc catctcgccg ttcaccgaga ccgccggcac cttcgtcaac gcggaaggcc
     3841 gcgtgcagtc gttccagggc acctgccggc cgctcggcga gacgcgcccg ggctggaagg
     3901 tgctgcgcgt cctcggcgac ctgctgggcc tgccgggctt cggccagcag tcgtccgaag
     3961 aagtgcttgc cgaggcgctg ggcgaccgcg cgacgctcgc cgcgcgcctg gacaaccgcg
     4021 ccgcgctgcc gtcgacgctg ccggccgacg gcgccgc
//
LOCUS       DASVLN010000661         4650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_143799, whole genome shotgun sequence.
ACCESSION   DASVLN010000661 DASVLN010000000
VERSION     DASVLN010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143799"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VF453_15620"
     CDS             complement(<1..909)
                     /locus_tag="VF453_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689143.1"
                     /translation="MALLDVRSLRVSFRTRGGEFVAVDGIDLQVDGDEVLAIVGESGS
                     GKSVAMLALMGLLPWTATVTADRMAFDGKELSALSPKERQAIVGRDVSMIFQEPITSL
                     NPCFTVGYQVGEVLKVHLGLDRAARRRRAVELLEQVGIPDPARRLRAFPHQLSGGMCQ
                     RVMIAMALACEPKLLIADEPTTALDVTIQAQILDLLLTLKRERRMALVLITHDLGLVA
                     EHADRVVVQYAGRQVESNAAAPLFADPHHPYTAALLAALPERAQARRLAAIPGVVPGQ
                     FDRPAGCLFSPRCEFADARCRAIVPAH"
     gene            complement(910..1812)
                     /locus_tag="VF453_15625"
     CDS             complement(910..1812)
                     /locus_tag="VF453_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7689144.1"
                     /translation="MAELLVEEALPPAPPSPAREFWAAFRENRGAVIGLAVVAFVALL
                     AILADVVSPHSPVEQFRDVVKVPPVWEDGGSWRFVLGTDSLGRDLLSRLIHGARVSLF
                     IGLAVMSVSFVIGVALGLASAALGNVVDVVVTRTMDLIMAVPSLVFAILVVAVLGPSL
                     ANTIVAVTIVYLPRYVRLVRASALAELRKEYVVAARVAGVGRLRLMLRTVLPNCLAPL
                     IVQAALGVSDAILEAAALGFLGLGAQPPIAEWGTMLADSREFIRSNPWMVTLPGLAIL
                     VTVVSINLAGDGLRDALDPKMRRS"
     gene            complement(1805..2815)
                     /locus_tag="VF453_15630"
     CDS             complement(1805..2815)
                     /locus_tag="VF453_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7689145.1"
                     /translation="MLKFLFKRLLLTIPTFFALMFVTFVAIRLVPGDPVEVRVGERGI
                     SPERLAMFRHELGLDQPVWLQFWHYVVQLLHGDFGTSLVTSQKVLTEFGTLFPATLEL
                     SFFGMLFAVVLGLPAGAVAAVKRGTWFDQVLMGLSVTGYSMPIFWWGLLLIMFVGERW
                     GWTPVSGRIDLINYYFEPRTGFMVIDAWLSGQAGAVKDALRHLILPSIVLGTIPLAVI
                     ARMTRSAMLEVLAEDYVRTARAKGLPAWRVFGVHAMRNALIPVVTVMGLQVGALLAGA
                     VLTETIFSWPGIGHWLIESISRRDYPALQGGVMLVSTMVIVVNLLVDVLYGVINPRIR
                     HG"
     gene            complement(2838..4451)
                     /locus_tag="VF453_15635"
     CDS             complement(2838..4451)
                     /locus_tag="VF453_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7689146.1"
                     /translation="MTRPASRGRHELAALLLAAFAALPLAASAKTFVYCSEGSPENFS
                     PALNTTGTSFDAARPVYDKLTEFARGTTRVEPGLAESWTISPDGRTYDFHLRKGVKFH
                     AGVNGFRPTREFNADDVIFSFERQWKPEHPYAKVSGGKYDFFADMGMPKLLQSITKVD
                     DHTVRFVLTQPDSTMLADLAMDFASIGSAEYADFLARQNRKEQFDQFPVGTGPFQFVM
                     YQKDAVIRFHRNADYWGEKALVDDLVYAITPDATARYSKLKAGECHFAIAPRPSDVAE
                     MQKDKSLKVISQPGLNIAYWAFNTQKPPFDKREVRQAFSMAIDKASIVRDVYLGAGQP
                     ARNLIPPTMWGYNDKVVDYKYDPAAAKALLAKAGVKTPLAVDLWYMPVQRPYNPNAKR
                     VAEMMQADLAKIGVQATLVTYEWGEYRKRLQQGEHMTGLMGWTGDNGDPDNFFILHSC
                     EAARPGGQNISKWCDKNFDERYLKAKTAADQATRIKLYQEMQQIEHDEAPDMKLAHST
                     VYEAMNARVTGFKQSPFGAHQFNGVDLAN"
BASE COUNT          754 a         1568 c         1655 g          673 t
ORIGIN      
        1 gtgcgcgggg acgatcgcgc ggcagcgggc gtcggcgaac tcgcagcgcg gcgagaacag
       61 gcaacccgcc gggcgatcga actgccccgg cacgacgccc gggatcgccg ccagccggcg
      121 tgcctgcgcg cgctcgggca gcgccgcgag caacgcggcc gtgtagggat ggtgcgggtc
      181 ggcgaacagc ggcgccgcgg cgttggactc gacctggcgg cccgcgtatt gcacgacgac
      241 gcggtcggca tgttccgcga ccaggccgag gtcgtgcgtg atcaggacca gcgccatgcg
      301 gcgctcgcgc ttgagcgtga gcagcaggtc gaggatctgg gcctggatcg tgacgtccag
      361 cgcggtggtc ggctcgtcgg cgatcagcag cttgggctcg caggcgagcg ccatcgcgat
      421 catcacgcgc tggcacatgc cacccgagag ctggtgcggg aatgcgcgca gccgccgcgc
      481 cgggtcgggg atgccgacct gctcgagcag ttccacggcc cgcctgcgac gcgcggcgcg
      541 atcgaggccc aggtgcacct tcagcacctc gccgacctgg tagccgaccg tgaagcatgg
      601 gttcagcgag gtgatcggtt cctggaagat catcgagacg tcgcgcccga cgatcgcctg
      661 gcgctccttc ggcgacaagg ccgagagctc cttgccgtcg aaggccatgc ggtcggccgt
      721 gaccgtcgcg gtccacggca gcaggcccat cagcgcgagc atcgcgaccg acttgcccga
      781 gcccgactcg cccacgatcg ccagcacctc gtcgccgtcg acctgcaggt cgatgccgtc
      841 cacggcgacg aactcgcccc cgcgcgtccg gaacgagacg cgcaggctgc gcacgtccag
      901 cagcgccatt caggacctcc gcatcttggg gtcgagcgcg tcgcgcaagc cgtcgcccgc
      961 caggttgatc gagacgaccg tgacgaggat cgccagtccc ggcagcgtca ccatccacgg
     1021 gttggagcgg atgaactcgc gcgaatcggc gagcatcgtg ccccattcgg cgatcggtgg
     1081 ctgcgcgccc aggcccagga agccgagcgc cgccgcctcg aggatcgcgt cggagacgcc
     1141 cagcgccgcc tgcacgatca gcggcgcgag gcagttgggc agcacggtgc gcagcatcag
     1201 gcgcagcctg ccgacgcccg cgacgcgggc ggcgacgacg tactccttgc gcagctcggc
     1261 cagcgccgag gcccgcacca ggcgcacata gcggggcagg tagacgatgg tcacggcgac
     1321 gatggtgttg gcgaggctcg ggcccagcac cgcgacgacc aggatcgcga acacgaggct
     1381 gggcacggcc atgatcaggt ccatggtgcg cgtgacgacc acgtcgacga cgttgcccag
     1441 cgcggcgctc gccagtccga gcgcgacgcc gatcacgaag gacacgctca tcacggccag
     1501 gccgatgaac agcgacaccc gcgcgccgtg gatcaggcgc gagagcaggt cgcgcccgag
     1561 gctgtcggtg cccagcacga agcgccagct gccgccgtcc tcccagaccg gcggcacctt
     1621 gacgacatcg cggaactgct ccaccggcga atgcggcgac acgacatcgg cgaggatcgc
     1681 cagcagcgcg acgaacgcaa ccactgccag cccgatgacc gcgccgcggt tctcgcggaa
     1741 cgcggcccag aactcgcggg cgggggaggg cggggccggc ggcagcgcct cctcgacgag
     1801 cagttcagcc atggcggatg cgtgggttga tcacgccgta gagcacgtcg acgagcaggt
     1861 tgaccacgat gaccatcgtc gagaccagca tcacgccgcc ctgcagcgcg gggtagtcgc
     1921 ggcgcgagat cgactcgatc agccagtggc cgatgccggg ccacgagaag atggtctcgg
     1981 tgagcaccgc gccggccagc agcgcgccga cctgcaggcc catcaccgtg acgaccggga
     2041 tcagcgcgtt gcgcatcgca tgcacgccga acacgcgcca ggcgggcagg cccttggcgc
     2101 gcgcggtgcg cacgtagtcc tcggccagca cctccagcat cgccgagcgc gtcatgcgcg
     2161 cgatgaccgc cagcgggatg gtgcccagca cgatggacgg caggatcagg tgccgcagcg
     2221 cgtccttgac ggcaccggcc tggcccgaga gccaggcgtc gatcaccatg aagcccgttc
     2281 tcggctcgaa gtagtagttg atcaggtcga tgcggccgga caccggcgtc cagccccagc
     2341 gctcgccgac gaacatgatc agcagcaggc cccaccagaa gatgggcatc gagtagccgg
     2401 tcaccgacag gcccatcagc acctggtcga accaggtgcc gcgcttgacc gccgcgaccg
     2461 cgccggcggg caggcccagc acgaccgcga acagcatgcc gaagaacgac agctccagag
     2521 tggccggaaa cagcgtgccg aactccgtca gcaccttctg gctggtcacg agcgaggtgc
     2581 cgaagtcccc gtgcagcagc tgcacgacgt agtgccagaa ctgcagccac accggctggt
     2641 cgagcccgag ctcgtggcgg aacatcgcca gccgctccgg gctgatgccg cgctcgccga
     2701 cccgcacctc caccggatcg cccggcacga ggcggatcgc gacgaaggtc acgaacatca
     2761 gcgcgaagaa ggtcggtatc gtcagcagca gccgcttgaa caggaacttc agcatgagcg
     2821 cgaggctggc cgcggactca gttggccagg tcgacgccgt tgaactggtg ggcgccgaac
     2881 gggctctgct tgaaaccggt cacgcgcgcg ttcatcgcct cgtagacggt cgagtgcgcg
     2941 agcttcatgt cgggcgcctc gtcgtgctcg atctgctgca tctcctggta cagcttgatg
     3001 cgcgtggcct ggtcggcggc ggtcttggcc ttgaggtagc gctcgtcgaa gttcttgtcg
     3061 caccacttcg agatgttctg gccgcccggg cgcgcggcct cgcagctgtg caggatgaag
     3121 aaattgtcgg ggtcgccgtt gtcgcctgtc cagcccatga ggccggtcat gtgctcgccc
     3181 tgctgcaggc gcttgcggta ctcgccccat tcgtaggtga cgagcgtggc ctgcacgccg
     3241 atcttggcga ggtcggcctg catcatctcg gcgacgcgct tggcgttcgg gttgtacggg
     3301 cgctgcaccg gcatgtacca gaggtcgacc gccagcggcg tcttcacgcc ggccttggcg
     3361 agcagtgcct tggcggcggc cgggtcgtac ttgtaatcga cgaccttgtc gttgtagccc
     3421 cacatcgtcg gcgggatcag gttcctggcc ggctggccgg cgccgaggta gacgtcgcgg
     3481 acgatggacg ccttgtcgat cgccatcgag aacgcctggc gcacctcgcg cttgtcgaac
     3541 ggcggcttct gggtgttgaa ggcccagtag gcgatgttca ggcccggctg gctgatcacc
     3601 ttgagggact tgtccttctg catctcggcg acgtcggacg gccgcggcgc gatcgcgaag
     3661 tgacattcgc cggccttgag cttggagtag cgcgcggtgg cgtccggcgt gatcgcgtac
     3721 accaggtcgt cgacgagcgc cttctcgccc cagtagtcgg cgttgcggtg gaagcggatc
     3781 accgcatcct tctggtacat cacgaactgg aacgggccgg tgcccaccgg gaattggtcg
     3841 aactgctcct tgcggttctg cctggcgagg aagtccgcgt attccgccga gccgatggag
     3901 gcgaagtcca tcgcgaggtc ggccagcatc gtcgagtcgg gctgcgtcag cacgaaccgg
     3961 acggtgtggt cgtcgacctt ggtgatcgac tgcagcagct tgggcatgcc catgtcggcg
     4021 aagaagtcgt acttgccgcc ggagaccttc gcgtacggat gttcgggctt ccactggcgc
     4081 tcgaacgaga agatcacgtc gtcggcattg aactcgcgcg tgggccggaa gccgttgacg
     4141 ccggcgtgga acttcacgcc cttgcgcagg tggaagtcgt acgtcctgcc gtcgggcgag
     4201 atcgtccagc tctcggccag gcccggctcg acgcgggtgg tgccgcgggc gaactcggtg
     4261 agcttgtcgt agaccgggcg cgccgcgtcg aagctggtgc cggtcgtgtt cagggcgggc
     4321 gagaagttct cgggcgagcc ctcggagcag tagacgaagg tcttggccga ggcggccagg
     4381 ggcagcgcgg cgaatgcggc caggagcagc gcggccagct cgtgacggcc acgcgaggcg
     4441 ggtcgggtca tcgaatcctc cgggtcaggg tgacgcatgg tagcgcgagc atgacgccgc
     4501 tgcgccgcgc cagggcaccg gttttccgag cattcgccgc cggcgcgcgc cgtggcaggc
     4561 gtcaaagtcc cgacagcggg atcgctgcgc cgcgctcgtc ggcgccgagc acctcgatga
     4621 ggcgggcgag gtcgccctcc agccgcgcga
//
LOCUS       DASVLN010000662        11480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_304143, whole genome shotgun sequence.
ACCESSION   DASVLN010000662 DASVLN010000000
VERSION     DASVLN010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11480
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304143"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VF453_15640"
     CDS             complement(<1..356)
                     /locus_tag="VF453_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX7689147.1"
                     /translation="MAIRWSTTRTLFVLAGIGVAAGIAGAVVSNERPARQPPAFPPAT
                     DPYAQGIYANGIVETDLPNGSNINLYPEVSGTVSAVLVQEGQAVHRGDAILRLDDTTA
                     RAQAAQQRAQAVAAQA"
     gene            complement(331..1083)
                     /locus_tag="VF453_15645"
     CDS             complement(331..1083)
                     /locus_tag="VF453_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689148.1"
                     /translation="MAAVAADIAQAPARAAARVAIAAAGVDKWFGDGDARTHALRAVS
                     LEARFGEMLYIVGPSGSGKTTLLSVISGILRPDGGRVVVDGQELWALDDDRLAEFRLR
                     RIGFVFQDYHLFPRLTTVENVAIPLILRKRPWNEALDAARAMLEVVGLSSRAELPPVK
                     LSGGEQQRVAIARAMVSAPDILILDEPTASLDGDTGKAIVGFVRREMLCATRTIVIVT
                     HDARIDEFADRVLRMEDGQIKDIDDGHPLVDD"
     gene            complement(1086..2222)
                     /locus_tag="VF453_15650"
     CDS             complement(1086..2222)
                     /locus_tag="VF453_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011853754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7689149.1"
                     /translation="MNGALLLAYKLLVNDRAKFGALLIGITFAVFLMVEMTSMFAGVL
                     HRSSSTVINIGARVWVMDPAVNNAAGAIPLPDTVLDAVRSIPGVRYAMPLFSGGALVK
                     MRDGTYQAATVLGLDDSTLLGRPELLQGRPEDIYAENGFIVVQDAEYAKLGRPQLGAE
                     FEINDHRAVVVGKARAAVGGLFGVPTLYTTYTRAVQYLPTARFTTAYVLVEPKSEADV
                     AEIQREVAGLGYVALTDDQFMRKISNFYTFQTGLGTNILLMTVISFIVGLSISGQTFY
                     TFILENLEKFGALKAIGAKSRELVAMILFQAGFTALTGFGLGVGLCAAAIALARWRIP
                     DYAALITPWNLLLALAMVIVIAGVSSIIGVRKVLRIEPFDIFRG"
     gene            2380..3129
                     /gene="phbB"
                     /locus_tag="VF453_15655"
     CDS             2380..3129
                     /gene="phbB"
                     /locus_tag="VF453_15655"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX7689150.1"
                     /translation="MSKDKRLALVTGGTGGLGEAICKRLFDGGFAVVALHTQGNGRVG
                     EWLAAREREGRAMVAQAVDVADYASCASAVDEIRRRFGPVSVLVNNAGITQDASFRKM
                     TAVQWQQVLRVNLDSMFNMTKQVIEDMLAAGWGRIVNISSVNGQRGAFGQANYAASKA
                     GIHGFTKSLAIEFASKGLTVNTVSPGYLRTPMVEKMPHEILESRIIPQIPVGRLGDPG
                     EIAELVAYLVSEQGAFVNGANIAINGGQHMA"
     gene            3152..3631
                     /locus_tag="VF453_15660"
     CDS             3152..3631
                     /locus_tag="VF453_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689151.1"
                     /translation="MSRRIDPVEAVPEIAEPAPPASAPEPQADPYVSQCLDWMRQFEH
                     VQGSAAKGWSTLIETAAERAASGHGWERWWDVASAFLREAVEETAKRQGEWFSAVAQF
                     QLQAAKMLVDLGDPSSHAAAEAADTQRSWLAQAWRDPAAVWFAPWLAGTAIRPVQVS"
     gene            3660..5414
                     /locus_tag="VF453_15665"
     CDS             3660..5414
                     /locus_tag="VF453_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7689152.1"
                     /translation="MAMELESPATRKVAGRQAAVRAPVRAATRAAPDPGAAWRDLDRL
                     REAGIGRITGGLSPEALALAWFDWAMHLAVAPGKRLQLALHDWSRPADASADPRFSDP
                     SWRLAPFDAMATAFLRCEDWWREATRGVPGTVPHHESVVAFASRQLLDMLSPSNLPWT
                     NPQVVARTIGQGGRNLWAGALHWMEDAARAAGLAADEEPRFVVGRDLAATPGKVVMRN
                     ELAELIQYAPATGVVEAEPVLIVPAWIMKYYILDLSAHDSLVRWLVGQGHTVFCLSWR
                     NVDESDRDLGMDDYRRLGVMAALDAVTRIVPGQRVHAAGYCLGGTLLAIAAAAMAGAG
                     DDRLRSLTLFAAQTDFSEPGELALFIDPAQVHMLDSLMWLRGYLTAGQMSWAFQMLRS
                     NDLVWSRMVHEYLMGERPGSNDLMAWNADATRMPYRMHSEYLHQLFLGNDLASNRYRV
                     DGHRVMLQRLRLPVFVVGTETDHVAPWRSVYKLHDLTDAPVTFVLCNGGHNAGIVNEP
                     GHPHRHFRMATRTGGVARLSADEWFEATSAQPGSWWTAWERWLVESGSHQRVPPPAMG
                     AAGYPPIDDAPGRYVMER"
     gene            5455..5916
                     /locus_tag="VF453_15670"
     CDS             5455..5916
                     /locus_tag="VF453_15670"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7689153.1"
                     /translation="MNRRTSSCLGIVAIAASLAACAGHREVSLAASPAAGSPYVGWRV
                     YNARCAACHGTDATGPAPVQGMLERVREMGAARFANLVLQRYDWPAAGDARGDADLRA
                     ALVEDILQHKEAAFEMPAWRDDPPVSAHILDLYAYLAARADGAVGTGRPAR"
     gene            6058..6279
                     /locus_tag="VF453_15675"
     CDS             6058..6279
                     /locus_tag="VF453_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689154.1"
                     /translation="MDMPSTKCQVAAEFELRFRSLFDEGRGYSFPCDRAGKVDLDALS
                     DRARNNYFFAHSVIGRDVAFPDVLPCAVH"
     gene            6293..8698
                     /locus_tag="VF453_15680"
     CDS             6293..8698
                     /locus_tag="VF453_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX7689155.1"
                     /translation="MTRIPSLTDAQRERIDAWWRAANYLSAGQIYLFDNPLLERALVA
                     QDIKRMHLGHWGTTPGQNFVYAHLNRLIAEHDLDMIYVSGPGHGGPAVVANTWLEGSY
                     SETYPDVSQDKAGMRRLFRQFSFPGGIPSHASPETPGSIHEGGELGYSLAHAFGAAFD
                     NPRLVVACVVGDGEAETGPLAAAWQSTRFLDAAHDGAVLPILHLNGFKTANPTLLARI
                     PREELVALLNGGGWEPHFVEGHEPAPMHEAMAAALDACIVRIRAIQEAARDGQPAQGH
                     RWPMVVLVSPKGWTGPRSVDGQAVEGSFRAHQVPIADPAGNPEHLRLLEDWLRSYRPQ
                     ELFHPDGRLRDEIAALAPKGERRMGANPHANGGTLLRPLRLPDFRDHAVDVSRRGMRA
                     PGDTRALGPFLRDVCVLNEPARNFRIFGPDETISNGLGAVLEKTARQWALPIRDGDEH
                     LAPGGRVMEVLSEHLCQGWLEGYVLTGRHGLFNCYEAFVPIVDSMFNQHAKWLEVTAR
                     LPWRRPVASLNILLSSHVWRQDHNGFTHQDPGFIDHVMNKKPEVVRVYLPPDANCLLS
                     VADHCLRSRHYVNVIVAGKHPSPQWLTMDEAAAHCAQGIGIWEWAGNEAEGCGPDVVF
                     ACCGDVPTLETLAAVQLLREHLPEVRVRVVNVVDLCRLMPRADHPHGLEDRQFDELFT
                     TDRPVVFAFHAYPWLVHRLTYRRANHANIHVRGYREEGTITTAFDMTVLNGIDRYHLA
                     IEAIDRLPSPGERKLAVTRRFRARLAQHRRWIESHGEDLPEVRDWTWHAGPGAAREPA
                     PHD"
     gene            8812..10938
                     /locus_tag="VF453_15685"
     CDS             8812..10938
                     /locus_tag="VF453_15685"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7689156.1"
                     /translation="MDDPSRWLAAVVEYSDDAIITKTLQGVITSWNPAAERLFGYAAA
                     EAVGRPVAMLVPADRADEEPRILARIANGERVDHFETERVRKDGSIVAVSVTISPVRD
                     AGGTIVGASKIARDIGERRKADARVQAQLARLFTLDQITRAIGEHQDQRSIYQVALRA
                     VEERLPLDFACICRVDEEGRQLVIAQVGLRSGRVGAELALSERAILPLEAEAWERWTD
                     GSLVYVRDLHGLEASPVRRLARGGFGSLVVAPLKVEGRVASLMIAARREADAFSSTDC
                     EFLLQLATHVALAAWHAQLHESLQRACEDLKAGREAAVRQQRLQALGEMAGGIVHDIG
                     NLVLPAAFFAQELMERETALSPDGSRGLELIKRCLDDVTAIVERLREFHRARDVAPRV
                     EAIDCNEVVRQVAALVRPRCAERRAQGVEVDVAVPACGPAAPIVGSPSEIREALVNLV
                     LNALDAMPGGGTATIMTRNRAADAVAGRPAAVEVVVQDTGVGMDEETRRRCMDPFFTT
                     KGEAGTGMGLAMVYGAVQRHGADITIDSAPGRGTTVTIAFACAGPAPGEAATRSDARG
                     AQADDGPVAPVSRAMRLLLVDDDPLVLETLQLMLQAGGHAITAVVGGRAGIEAFRDAL
                     GRGTPFDVVVTDLGMPEVDGWAVARAVKRLASATPVVLLTGWGEGLQRNASAGRHVDA
                     VLGKPPRRGDLDEALAHVARPRESVS"
     gene            11039..>11480
                     /locus_tag="VF453_15690"
     CDS             11039..>11480
                     /locus_tag="VF453_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689157.1"
                     /translation="MSYKTVLLHVADDAHLEAHADYATRLARLFDSRLLGLTCHRPSP
                     WPQDGAVAFLGGDPLTVELRAAEEAAVAREATFLRRCRLAEVTSFEAIREDAASATAI
                     CERALGADLVLLEQPEPATKAYAERRAVVDAVLADSPRPVLMLPY"
BASE COUNT         1621 a         4126 c         4137 g         1596 t
ORIGIN      
        1 tgggcctgag cggccacggc ctgcgcccgc tgctgcgccg cctgcgcgcg cgccgtcgtg
       61 tcgtccaggc gcaggatcgc gtcgccccgg tgcaccgcct gtccttcctg cacgagcacg
      121 gccgagacgg tgcccgagac ctcgggatag aggttgatgt tcgatccgtt gggcaggtcc
      181 gtctcgacga tgccgttcgc atagatgccc tgcgcgtagg gatcggtcgc cggcgggaag
      241 gcgggcggct gccgggccgg ccgttcgttc gacaccacgg cccccgcgat gccggccgcg
      301 acgccgatgc ccgcgaggac gaacagcgtc ctagtcgtcg accagcggat ggccatcgtc
      361 gatgtccttg atctgcccgt cctccatgcg caggacgcgg tcggcgaact cgtcgatgcg
      421 cgcgtcgtgc gtcacgatca cgatggtgcg cgtcgcgcag agcatctccc gccgcacgaa
      481 gcccacgatc gccttgcccg tgtcgccgtc cagcgaggcg gtcggctcgt cgaggatgag
      541 gatgtcgggc gcgctcacca tcgcgcgcgc gatcgccacg cgctgctgct cgccgccgga
      601 cagcttgacc ggcggcagct cggcgcgcga cgacaggccc acgacctcca gcatcgcgcg
      661 ggcggcgtcc agcgcctcgt tccagggccg cttgcgcagg atcagcggga tggccacgtt
      721 ctcgaccgtc gtcaggcgcg ggaagaggtg gtagtcctgg aacacgaagc cgatgcgccg
      781 gagccggaac tcggcgaggc ggtcgtcgtc cagcgcccag agctcctggc cgtcgacgac
      841 cacccgcccg ccgtcgggcc gcaggatgcc cgagatgacc gagagcagcg tcgtcttgcc
      901 gctgcccgac gggccgacga tgtacagcat ctccccgaat cgtgcctcca gcgacaccgc
      961 gcgcagcgcg tgcgtgcgcg cgtcgccgtc gccgaaccac ttgtccacgc cggccgcggc
     1021 gatggccacc cgggcggcgg cccgggccgg cgcttgcgcg atgtccgccg cgaccgccgc
     1081 caccgtcagc cccggaagat gtcgaacggc tcgatgcgca gcaccttgcg cacgccgatg
     1141 atgctcgaga cgccggcgat cacgatcacc atcgccagcg cgagcagcag gttccagggc
     1201 gtgatcagcg cggcgtagtc gggaatccgc cagcgggcga gggcgatggc cgcggcgcac
     1261 agcccgacgc cgaggccgaa gccggtgagc gccgtgaagc cggcctggaa caggatcatg
     1321 gcgaccagct cccggctctt ggcgccgatg gccttgagcg cgccgaactt ctccaggttc
     1381 tcgaggatga aggtgtagaa ggtctggccc gagatcgaca gccccacgat gaagctgatc
     1441 accgtcatca gcaggatgtt ggtgcccagg ccggtctgga acgtgtagaa gttcgagatc
     1501 ttccgcatga actggtcgtc ggtcagcgcg acgtagccca ggcccgcgac ctcgcgctgg
     1561 atctcggcga cgtcggcctc ggacttgggc tcgacgagga cataggcggt cgtgaagcgg
     1621 gcggtgggca ggtattgcac cgcgcgcgtg tacgtggtgt agagcgtcgg cacgccgaac
     1681 agcccgccga ccgcggcgcg cgccttgccg accacgaccg cgcgatggtc gttgatctcg
     1741 aactcggcgc cgagctgcgg ccgccccagc ttcgcgtact cggcgtcctg cacgacgatg
     1801 aagccgttct cggcgtagat gtcctccggg cggccctgca gcagctcggg ccggccgagc
     1861 agggtgctgt cgtccaggcc cagcacggtc gccgcctggt aggtgccgtc gcgcatcttc
     1921 acgagcgcgc cgccggagaa cagcggcatg gcgtagcgca cgccggggat gctgcgcacc
     1981 gcgtccagga cggtgtcggg cagcgggatc gcgccggccg cgttgttgac ggccggatcc
     2041 atgacccaga cccgcgcgcc gatgttgatg accgtcgacg acgaccggtg caggacgccc
     2101 gcgaacatcg acgtcatctc caccatgagg aagaccgcga acgtgatgcc gatcagcagc
     2161 gcgccgaact tcgcgcggtc gttgaccagc agcttgtagg ccagcaggag ggcgccgttc
     2221 atgaggggcg agtgttcgcg cgccggcgcc gggcggcctt gcgcccgctc aatcgcggca
     2281 cggcttgagg cggatcaacg ccccgtccgg caacgagcct agtctcggcc tccatgccgg
     2341 cgccggacct gcggcgggct gttcgacaag ggatccccca tgagcaagga caagcgactc
     2401 gccctcgtca cgggcggcac gggcggcctc ggcgaggcca tctgcaagcg gctgttcgac
     2461 ggcggcttcg cggtcgtcgc gctccacacc cagggcaacg gccgcgtcgg cgaatggctg
     2521 gccgcgcgcg agcgcgaggg gcgcgccatg gtggcgcagg cggtcgacgt cgccgactac
     2581 gcctcgtgcg cgtccgcggt cgacgagatc cggcgccggt tcgggccggt ctcggtgctc
     2641 gtcaacaacg ccggcatcac gcaggacgcc agcttccgga agatgacggc cgtgcagtgg
     2701 cagcaggtgc tgcgggtcaa cctcgactcg atgttcaaca tgaccaagca ggtgatcgag
     2761 gacatgctgg cggccggttg gggacgcatc gtcaacatct cgtcggtcaa cgggcagcgc
     2821 ggcgcgttcg ggcaggcgaa ctacgcggcc tccaaggcgg gcatccacgg cttcacgaag
     2881 tcgctcgcga tcgagttcgc gagcaagggc ctcaccgtca acaccgtctc gcccggctac
     2941 ctgcgcacgc cgatggtcga gaagatgccg cacgagatcc tcgagtcgcg gatcatcccg
     3001 cagatccccg tcggccggct cggcgacccg ggggagatcg ccgagctggt ggcctacctc
     3061 gtgtccgagc agggcgcctt cgtcaacggc gccaacatcg ccatcaacgg cggccagcac
     3121 atggcctgac cggccgtccc aggagatcac gatgagcaga aggatcgacc ccgtcgaggc
     3181 ggtgcccgag atcgccgaac cggccccgcc ggcgagcgcg ccggagccgc aggcggatcc
     3241 ctacgtctcc cagtgcctgg actggatgcg ccagttcgag cacgtgcagg gcagtgccgc
     3301 caagggctgg agcaccttga tcgagaccgc ggcggagcgc gccgcgtcgg gccacgggtg
     3361 ggagcggtgg tgggacgtcg ccagcgcgtt cctgcgggaa gcggtcgagg agacggccaa
     3421 gcgccagggc gaatggttct cggcggtcgc gcagttccag ttgcaggctg ccaagatgct
     3481 ggtggacctg ggcgacccgt cgtcgcatgc cgccgccgag gccgccgata cgcagcggtc
     3541 gtggctggcg caggcctggc gcgatcccgc cgcggtctgg ttcgcgccct ggctcgccgg
     3601 gacggcgatc cgcccggtcc aggtctcctg aagcgatgcc gcgagggaag gtggacgaca
     3661 tggccatgga gctcgagtct cccgcgacac ggaaggtggc cgggcggcag gcggcggtgc
     3721 gcgcgccggt gcgtgcggca acgcgcgcgg cgccggatcc gggcgcggcc tggcgcgatc
     3781 tcgaccgcct gcgcgaggcc gggatcggcc ggatcacggg cggtctctcg cccgaggcgc
     3841 tcgcgctcgc ctggttcgac tgggcgatgc atctggcggt ggcgcccggc aagcgtctgc
     3901 agctcgcgct gcacgactgg agccggccgg cggacgccag cgcggatccg cgcttctcgg
     3961 atccgtcgtg gcggctcgcg ccgttcgacg cgatggccac ggccttcctg cgttgcgagg
     4021 actggtggcg cgaggccacg cgcggggtgc ccggcaccgt gccgcaccac gagtccgtcg
     4081 tcgcgttcgc gagccggcaa ctgctggaca tgctgtcgcc gtccaacctc ccgtggacca
     4141 atccgcaggt cgtggcccgc acgatcgggc agggcgggcg caacctctgg gcgggcgcgc
     4201 tgcactggat ggaggacgcc gcccgcgccg ccggcctcgc ggccgacgag gagccccgct
     4261 tcgtggtcgg gcgcgacctc gccgcgacgc cgggcaaggt ggtgatgcgc aacgagctcg
     4321 ccgagctgat ccagtacgcg ccggcgaccg gcgtcgtcga ggccgagccg gtgctgatcg
     4381 tgccggcatg gatcatgaag tactacatcc tcgacctgtc ggcgcacgac tcgctggtgc
     4441 gctggctggt cgggcagggc cacacggtgt tctgcctgtc ctggcgcaac gtcgacgagt
     4501 cggatcgcga cctgggcatg gacgactacc ggcgcctcgg cgtgatggcg gcgctggacg
     4561 cggtgacgcg catcgtgccc ggccagcgcg tgcacgcggc cggctactgc ctcggcggca
     4621 cgctgctggc catcgcggcc gccgccatgg cgggcgcggg cgacgaccgg cttcgcagcc
     4681 tcacgctgtt cgcggcgcag accgacttca gcgaacccgg cgagctggcg ctgttcatcg
     4741 acccggcgca ggtgcacatg ctcgacagcc tgatgtggct gcgcggctac ctgacggcgg
     4801 ggcagatgtc ctgggccttc cagatgctgc gctccaacga cctggtctgg tcgcgcatgg
     4861 tgcacgaata cctgatgggc gaacggccgg gatccaacga cctcatggcc tggaacgccg
     4921 acgcgacgcg catgccctat cgcatgcact ccgagtacct gcaccagctg ttcctcggca
     4981 acgaccttgc ctccaaccgc taccgggtcg acggccaccg cgtgatgctg cagcgcctgc
     5041 gcctgcccgt cttcgtcgtc ggcacggaga ccgaccacgt ggcgccgtgg cggtcggtgt
     5101 acaagctgca cgacctcacc gacgcgccgg tcaccttcgt gttgtgcaac ggcggccaca
     5161 acgcgggcat cgtcaacgag cccggccacc cgcatcgcca tttccgcatg gccacgcgca
     5221 ccgggggcgt ggcgcgactg agtgccgacg agtggttcga ggcgacgtcc gcccagcccg
     5281 gctcgtggtg gacggcgtgg gagcgctggc tcgtcgagag cgggagccac cagcgcgtgc
     5341 cgccgccggc catgggtgcg gcgggctatc cgccgatcga cgatgcgccg ggccgctacg
     5401 tgatggagcg ctgaggtgcg cacgcggcgc ggaccgcatc gccagggaga gggcatgaat
     5461 cgtcggacat cgtcctgcct cgggatcgtc gccatcgcgg cctccctggc ggcctgcgcg
     5521 ggacatcgcg aggtctcgct cgccgcgtcg ccggccgcgg gctcgcccta cgtcggctgg
     5581 cgcgtctaca acgcccgctg cgcggcctgc cacggcaccg acgcgaccgg gccggcgccc
     5641 gtgcagggca tgctcgagcg cgtccgcgag atgggcgccg cgcgcttcgc caacctcgtg
     5701 ctgcagcgct acgactggcc cgcggccggc gacgcgcggg gcgatgccga cctccgcgcg
     5761 gcgctggtcg aggacatcct ccagcacaag gaggccgcct tcgagatgcc cgcgtggcgg
     5821 gacgacccgc cggtcagcgc gcacatcctc gacctgtatg cctacctcgc ggcgcgcgcg
     5881 gacggcgcgg tcggcaccgg ccgccccgcg cgctgacgcc cgccacccgt cacccgtcac
     5941 ccgtcacccg tcacccgtca cccgttgttt cgatgtgttg cccggcattg acgcgccgtt
     6001 ttcggcgcgc gcctaccttg cagtcagacg atccttcgtc gccgccagga gcgcgccatg
     6061 gacatgccaa gcacgaaatg ccaagtcgcc gcggaattcg agcttcgatt ccgctcgttg
     6121 ttcgacgagg gccgcgggta cagcttcccc tgcgaccgcg ccggcaaggt cgatctcgac
     6181 gccctgagcg accgggcgcg gaacaactac ttcttcgccc acagcgtcat cgggcgcgac
     6241 gtcgcgtttc cggacgtgct gccgtgcgcg gtgcattgac ccaggagccg tcatgacccg
     6301 gatcccatcg ctcaccgacg cgcagcgcga gcgcatcgac gcctggtggc gggcggccaa
     6361 ctacctgtcg gcggggcaga tctacctctt cgacaacccg ctgctcgagc gcgcgctcgt
     6421 ggcccaggac atcaagcgca tgcacctggg ccactggggg acgacgccgg gccagaactt
     6481 cgtctacgcg cacctgaacc ggctgatcgc cgagcacgac ctcgacatga tctacgtcag
     6541 cggcccgggc cacggcggcc cggcggtcgt cgcgaacacg tggctcgagg ggagctacag
     6601 cgagacgtac cccgacgtgt cgcaggacaa ggccggcatg cgacggctgt tccggcagtt
     6661 ctcgtttccc ggcggcatcc ccagccacgc gtcgcccgag acgcccggct cgatccacga
     6721 aggcggcgag ctcggctatt cgctcgcgca cgcgttcggc gccgccttcg acaatccgcg
     6781 cctggtcgtc gcctgcgtgg tcggcgacgg cgaggccgag accgggccgc tcgccgcggc
     6841 ctggcagtcc acgcgcttcc tggatgcggc gcacgacggc gcggtgctgc cgatcctgca
     6901 cctgaacggc ttcaagaccg ccaatccgac gctgctcgcc cggatcccgc gcgaggagct
     6961 ggtggcgctg ctgaacggcg gcggctggga gccgcacttc gtcgagggac acgagcccgc
     7021 gccgatgcac gaggcgatgg ccgcggcgct cgacgcttgc atcgtccgga tccgggccat
     7081 ccaggaggcc gcgcgcgacg gccagccggc gcaaggccat cggtggccga tggtcgtgct
     7141 ggtttcgccc aagggctgga cgggcccgcg aagcgtcgac gggcaagcgg tcgagggcag
     7201 cttccgggcg caccaggtgc cgatcgccga tcccgccggc aatcccgagc acctgcgcct
     7261 gctcgaggac tggctgcgca gctatcgccc gcaggagctg ttccatcccg acggccgcct
     7321 gcgcgacgag atcgcggcgc tcgcgccgaa gggcgagcgg cgcatgggtg ccaacccgca
     7381 cgccaacggc ggcacgctcc tgcgcccgct gcggctgccc gacttccgcg accatgcggt
     7441 cgacgtgtcg cggcgcggca tgcgcgcgcc gggcgacacg cgcgcgctgg ggcccttcct
     7501 gcgggacgtc tgcgtcctga acgagccggc ccgcaacttc cggatcttcg ggccggacga
     7561 gacgatctcg aacggcctgg gcgcagtgct cgagaagacc gcgcgtcaat gggcgctgcc
     7621 gatccgcgac ggcgacgagc acctggcgcc cggcggccgg gtgatggagg tgctctcgga
     7681 gcacctgtgc cagggctggc tcgagggcta cgtgctgacg ggacggcacg ggctgttcaa
     7741 ttgctacgag gcgttcgtgc cgatcgtcga ctcgatgttc aaccagcacg ccaagtggct
     7801 cgaggtgacc gcgcggctgc cttggcggcg tcccgtcgcg tcgctcaata tcctgctgtc
     7861 gtcgcatgtc tggcgccagg accacaacgg cttcacgcac caggatcccg gcttcatcga
     7921 ccacgtcatg aacaagaagc ccgaggtcgt gcgcgtgtac ctgccgccgg acgcgaattg
     7981 cctgctgtcg gtcgccgacc attgcctgcg cagccgccac tacgtcaacg tgatcgtcgc
     8041 cggcaagcat ccgtcgcccc aatggctgac gatggacgag gccgccgcgc actgcgccca
     8101 gggcatcggc atctgggagt gggccggcaa cgaggcggag ggctgcggcc ccgacgtcgt
     8161 cttcgcgtgc tgcggggacg tgccgaccct ggagacgctc gccgcggtgc aactgctgcg
     8221 cgagcacctg cccgaggtgc gcgtgcgcgt cgtcaacgtc gtggacctgt gccgcctgat
     8281 gccgcgcgcg gaccatccgc acggcctcga ggatcggcag ttcgacgagc tgttcacgac
     8341 cgacaggccg gtcgtcttcg cgttccacgc ctatccctgg ctcgtccacc ggttgacgta
     8401 ccggcgggcc aaccacgcca acatccacgt ccgcggctac cgggaggaag ggacgatcac
     8461 gacggcgttc gacatgaccg tcctgaacgg gatcgaccgc taccacctgg ccatcgaggc
     8521 gatcgaccgc ctgccttcgc ccggcgaacg caagctcgcg gtgacgcgtc gcttccgggc
     8581 caggctcgcg cagcaccggc gatggatcga gtcgcatggt gaggacctgc ccgaggtgcg
     8641 cgattggacg tggcacgccg gccctggcgc cgcgcgcgag cctgcccccc acgattgatg
     8701 cctccatgtt ggccgcaggg ggatggccca agcgcccgtc aggcctagac tgcgagcgcc
     8761 gaggcggccg tgcgcggtcc cggcgcttcc gcaaccctcc ggaggccggt catggacgac
     8821 cccagccgct ggctcgccgc cgtcgtcgaa tattcggacg acgccatcat caccaagacg
     8881 ctccagggcg tcatcacgag ctggaatccc gcggccgagc ggctgttcgg ctatgcggcg
     8941 gcggaagccg tcggccggcc cgtcgcgatg ctggtgccgg ccgaccgggc ggacgaggag
     9001 ccgcggatcc tcgcgcggat cgccaatggc gagcgcgtcg accacttcga gaccgaacgc
     9061 gtccgcaagg acggctccat cgtcgcggtc tccgtgacga tctcgcccgt gcgcgacgcc
     9121 ggcgggacca tcgtcggcgc ctccaagatc gcgcgcgaca tcggcgagcg ccgcaaggcc
     9181 gatgcgcggg tgcaggcgca gctcgcgcgc ctgttcacgc tcgaccagat cacgcgcgcc
     9241 atcggcgagc accaggacca gcgcagcatc taccaggtcg cgctccgcgc ggtcgaggag
     9301 cgccttccgc tcgacttcgc ctgcatctgc cgcgtcgacg aggaaggccg ccagctggtg
     9361 atcgcgcagg tcggcctgcg cagcggccgc gtcggcgcgg agctggccct gtcggagcgc
     9421 gccatcctgc cgctggaggc cgaggcctgg gagcgctgga cggacggcag cctggtctac
     9481 gtgcgcgacc tgcacgggct cgaggcctcg ccggtccgcc gcctcgcgcg gggtggcttc
     9541 ggctcgttgg tggtcgcgcc cttgaaggtc gagggccgtg tcgcgtccct gatgatcgcg
     9601 gcccggcgcg aggccgacgc cttctcgagc accgactgcg aattcctgct gcagctcgcg
     9661 acgcacgtcg ccctggcggc ctggcatgcg cagctgcacg aatcgctgca gcgggcgtgc
     9721 gaggacctga aggccgggcg ggaggccgcc gtgcgccagc aacggctgca ggcgctcggc
     9781 gagatggcgg gcggcatcgt ccacgacatc ggcaacctcg tcctgccggc cgcgttcttc
     9841 gcccaggagc tgatggagcg cgagacggcc ctctcgccgg acggctccag gggcctggag
     9901 ctcatcaagc gctgcctgga cgacgtgacc gcgatcgtgg agcgcctgcg tgagttccat
     9961 cgcgcccggg acgtcgcgcc gcgcgtggag gccatcgact gcaacgaggt cgtccggcag
    10021 gtcgcggcgc tggtccgccc gcgatgcgcc gagcggcgcg cgcaaggggt cgaggtcgac
    10081 gtggcggtgc cggcatgcgg gccggccgcc ccgatcgtcg gctctccgtc cgagatccgc
    10141 gaggcgctgg tcaacctcgt gttgaacgcg ctcgacgcca tgcccggcgg cggcacggcc
    10201 acgatcatga cccgcaaccg cgccgccgac gcggtcgccg ggcgcccggc agccgtcgag
    10261 gtcgtcgtcc aggacacggg tgtcggcatg gacgaggaga cgcggcgccg ctgcatggat
    10321 ccgttcttca cgacgaaggg cgaagccggc accgggatgg gcctggccat ggtctacggc
    10381 gcggtgcagc ggcatggtgc cgacatcacg atcgacagcg cgcccggccg gggaacgacg
    10441 gtgacgatcg ccttcgcgtg cgccggtccg gcgccgggcg aggcggcgac gcgatcggac
    10501 gcgcgggggg cgcaggccga cgacggcccg gtcgcgccgg tctcgcgcgc gatgcggctg
    10561 ctgctggtcg acgacgatcc gctcgtgctc gagacgctcc agctcatgct gcaggccggc
    10621 gggcatgcga tcaccgccgt cgtcggggga cgcgcaggca tcgaggcctt ccgcgacgcc
    10681 ctgggccgcg ggacgccgtt cgatgtcgtc gtcaccgacc tcggcatgcc cgaggtcgac
    10741 ggctgggccg tcgcgcgcgc cgtcaagcgg ctggcgtcgg ccacgcccgt cgtgctgttg
    10801 accgggtggg gcgagggact gcagcgcaac gcgagcgccg gtcgccacgt cgacgccgtg
    10861 cttggcaagc cgccgcgaag gggcgacctc gacgaggcgc tcgcgcacgt ggcgcggccg
    10921 cgcgaatcgg tgtcctgagc ggcgcggcgg cgtgcggggc aagcgcggga actgcgctgc
    10981 gtcaatcgcg cgcgcgaacg gtggcgaaga atggccggca tcccgccccg gaggccccat
    11041 gagctacaag accgtcctgc tccacgtcgc ggacgacgcg cacctggagg cgcacgccga
    11101 ctacgccacg cgcctcgccc gcctgttcga cagccgcctg ctcggactca cctgccaccg
    11161 gcccagcccc tggccgcagg acggcgcggt ggccttcctc ggcggcgatc cgctgacggt
    11221 cgagctccgc gccgccgaag aggcggccgt ggcgcgcgag gcgacctttc tccgccgatg
    11281 ccggctggcc gaggtgacgt cgttcgaggc catccgcgag gacgcggcgt ccgccacggc
    11341 gatctgcgag cgcgcgctcg gcgccgacct cgtgctgctg gaacagcccg agccggcgac
    11401 caaggcctac gcggagcgcc gggccgtcgt cgacgcggtg ctcgccgaca gcccccggcc
    11461 cgtgctcatg ctgccctacg
//
LOCUS       DASVLN010000663         4898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_304214, whole genome shotgun sequence.
ACCESSION   DASVLN010000663 DASVLN010000000
VERSION     DASVLN010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304214"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            566..703
                     /locus_tag="VF453_15695"
     CDS             566..703
                     /locus_tag="VF453_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689158.1"
                     /translation="MTHDRVMRFEPTFRSRGQALRFAWIQARAWLDAQAPTPAPIPGG
                     Q"
     gene            706..966
                     /gene="infA"
                     /locus_tag="VF453_15700"
     CDS             706..966
                     /gene="infA"
                     /locus_tag="VF453_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX7689159.1"
                     /translation="MSREELIEMRGVVDEMLPDSRFRVTLENGHALIAYTGGKLRKNR
                     IRILAGDQVSLELSPYDLTKGRITFRHLDRQGPRPGTRPGRR"
     gene            1079..1288
                     /locus_tag="VF453_15705"
     CDS             1079..1288
                     /locus_tag="VF453_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7689160.1"
                     /translation="MTIQTGTVKWFNEAKGFGFIAPDDGGKDLFAHFKEIQGTGFKVL
                     AENQRVEFEAGQGQKGPQAMRIRAL"
     gene            1353..1532
                     /locus_tag="VF453_15710"
     CDS             1353..1532
                     /locus_tag="VF453_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689161.1"
                     /translation="MPPKPNYAFEKRQRELEAKRKKEEKAARKSRPASEAGGEGTAPA
                     DSGDASPPGSPGTPG"
     gene            complement(1583..2014)
                     /locus_tag="VF453_15715"
     CDS             complement(1583..2014)
                     /locus_tag="VF453_15715"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEX7689162.1"
                     /translation="MGLLDSIVGQVSGALAGAAPGAGQVHPGLLEVVTSMLSSPPGNG
                     APGLQGLVQQFEQQGLGHLVQSWIGTGANLAITPEQVQAVLGEPRIQAVASKLGLSAA
                     DVANQLAGLLPHAVDAATPDGSPAAGNVLSEALNAFAALRR"
     gene            2340..4301
                     /locus_tag="VF453_15720"
     CDS             2340..4301
                     /locus_tag="VF453_15720"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7689163.1"
                     /translation="MTALMKLLRRARANPRVPLVVLALLLVVAGIVLLGSAASREAQA
                     RKSPDARAFERAPEAWLDHQRSVSDFVQAADGRRLAAVALANAHPGLVLYTLKDGSKA
                     SVLVPGCTVIGCAGTALDHLAERAAAGGFALVGVDVDPRTPSQHVLDALATLVSPAIL
                     IVTLVGGFFMLARLQTGAGGAVARLSERPKTQFADAIGNDEAKAALQRVKAFLHDPSA
                     YAKLGAAAPRGVLLVGPPGTGKTLLAKALAGESQANFIAVDGSYFTATYYGAGVAKVK
                     ALFKQARKSAPCVLFIDEVDGIGKRSRNAEHGGENEMNRIINRVLVEMDGFEPMDNVV
                     VVAATNHENNIDEAMRRPGRFDMLVRLAKPTLPERAKLFDLYLGKVVHDGSADTASLA
                     RMTPGVSPAEIANIVNKAASSAAERGAEKVSADHVLRAIETHLLGGEVSPVKDLLTEA
                     TRVRLAYHEAGHALVGHWTGAGLVERVTIEPRGPALGVTYMTRDTEDPLYRQGELVSR
                     LAMMLGGREAELLVQGNVSSGASDDLKRASELAIEMVGSLGFSETFGLLSVGGIPDKL
                     LGPDVQAALLKEARTLLEDAQGTCRRILNAQRARLDAMAHALLNCEVLAGEALKTMLG
                     DVHAVEDAVVVAPKPAPALPAPAAELETA"
     gene            4395..>4898
                     /locus_tag="VF453_15725"
     CDS             4395..>4898
                     /locus_tag="VF453_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HEX7689164.1"
                     /translation="MQRIRRVLAACVPFAAIVAVFAAAVAWHLGEPQRQVDRFHRLLA
                     DDAPLARDYGDDARVVLTRRGCARQCSAYTVSITGAGVVEFAGRADVCATAPTTDQVP
                     RGTAQRLVDAMVRAGFDDLPTNGAMARRGPGEQGSVVILEVGGRRHAVDDRQLPERPL
                     LAAIEQQV"
BASE COUNT          697 a         1854 c         1695 g          652 t
ORIGIN      
        1 cgccgctggg cggcgagtcc cggctggccg tcagccggcg cctgttcggg acgctgcacg
       61 agctcggccg ctccgagggc gagcgctgga tcgcgcgcca cctgcgcgac gtcggccggc
      121 gcgacagcgt cgacctgcag gcggtcttcg cgtgacggag gccggcggca accgtccctg
      181 ctgtgatata ttcgaatcct tcctcgcgat tcgttgccgc ggagcacgcc tcccgccatc
      241 gcgccgagat cacgagtcga tctccttccc ttgccagacg ctcgatcgcc gacgcggtcg
      301 tcctgttctg gtcgccgtcg accgcagcga acgaccctgc atcgaccgcc ggacagcgat
      361 ccacccgccg cctgcgcgcc cgcgcagccc accagcgtcc accgatgcct gcgcccgccc
      421 gacagtggcg tgcgcgcgcc gacaaggaaa tcccattcaa gaagccaccc gacagatcgg
      481 gaagtacctc gtttcttcct ccacccgccc cgccgacggc ggccgctacg ccgccgccct
      541 gtccatccgc agcgggcgcg gcagcatgac ccacgaccgc gtgatgcggt tcgagcccac
      601 cttccgctcg cgcggccagg ccctgcgctt cgcgtggatc caggcgcgcg cctggctcga
      661 cgcgcaggcg cccacgcccg cgcccatccc gggagggcag tgaccatgtc ccgcgaagaa
      721 ctgatcgaga tgcgcggcgt cgtcgacgaa atgctgcccg actcgcgctt ccgcgtgacg
      781 ctcgagaacg gccacgcgct catcgcctac acgggcggca agctgcgcaa gaaccgcatc
      841 cgcatcctcg ccggcgacca ggtgtcgctc gagctctcgc cctacgacct gacgaagggg
      901 cggatcacct tccgccacct ggatcgccag ggcccccggc ccggcacgcg tccgggtcgc
      961 cggtgaaccg cggccttcac cggccgcgac gcgcccggcc tccgcgttcc cgaggccccc
     1021 gatccgcgcc ggcggctacc ggcgcatccc acccgaattc gcatagaaag gattcatcat
     1081 gaccatccag accggaaccg tgaaatggtt caacgaggcc aagggcttcg gcttcatcgc
     1141 gcctgacgac ggcggcaagg acctgttcgc ccacttcaag gaaatccagg gcacgggctt
     1201 caaggtgctc gcggagaatc agcgcgtcga gttcgaagcc ggccagggcc agaagggccc
     1261 gcaggccatg cgcattcgcg cgctctgata atccggcgtc cggcgaggcg tcgccggacc
     1321 gtcctggaac cacacgcacc ggaggatccc gcatgccccc caaacccaac tacgcgttcg
     1381 agaagcgcca gcgcgagctc gaggcgaagc gcaagaagga ggaaaaggcg gcacgcaagt
     1441 cccgcccggc ctccgaggcc ggtggcgagg gcacggcgcc ggcggattcc ggcgacgcgt
     1501 cgccgcccgg atcgcccggc acgccgggct gacgccgggc ccatcccggc cacgccgacc
     1561 gacgcgtcgg ccgccggggc gttcagcgcc gcagcgccgc gaaggcgttg agcgcctcgc
     1621 tcagcacgtt gcccgctgcc ggagagccat cgggcgtcgc ggcgtcgact gcgtgcggca
     1681 gcagccctgc gagctggttg gcgacgtccg ccgcggacag cccgagcttg ctcgcgaccg
     1741 cctggatgcg cggctcgccc agtaccgcct gcacctgctc gggcgtgatg gccaggttcg
     1801 cgccggtgcc gatccatgac tgcacgaggt ggccgaggcc ctgctgctcg aactgctgca
     1861 cgagcccctg caggcccggc gcgccgttcc ccggcgggct cgagagcatg gaggtgacga
     1921 cctccagcag gcccggatgc acctgtcccg cgcccggcgc cgcgcccgcg agcgcgcccg
     1981 agacctgtcc gacgatcgaa tccagcaagc ccatgatcct gctcctgcaa cgacgggggc
     2041 gcgcgtgcgc cgatgtgccg caatgtagcg ctgcgccggc gccgtgacca tgttccttcg
     2101 gctggccagg gccgccgcgg cgaccgccgc cgggcggctg cgcgaccatg gcccgctgcc
     2161 gcgttgcgcg ggcgccgcac ggtcgcgcaa cgcggtgtcg ggagccttga acagggtgcg
     2221 cgaggcatgg cttttcccgc ggaacttcac ctgatccggg gttccaatgg aggccggtat
     2281 cgcccgatac cgaggatcgc ctttgccgcg ctccgccgac gtctccacga gtccctgcca
     2341 tgaccgcctt gatgaagctt ctccgccgcg cccgcgccaa tccgcgcgtg cccctcgtcg
     2401 tcctcgcgct gctgctggtg gtcgcgggca tcgtgctgct cggcagcgcg gcttcgcgcg
     2461 aggcgcaggc gcgcaagagc cccgacgcgc gcgccttcga acgcgcgccc gaggcgtggc
     2521 tggaccacca gcgcagcgtc tccgacttcg tgcaggcggc ggacggccgg cgcctggccg
     2581 cggtggcgct cgccaatgcg catccgggcc tcgtgctgta caccctcaag gacggcagca
     2641 aggccagcgt gctcgtgccg ggctgcaccg tgatcggctg cgccggcacc gcgctcgacc
     2701 acctcgccga acgcgccgcc gccggtggct tcgcgctggt gggcgtcgac gtcgacccgc
     2761 gcacgcccag ccagcacgtg ctggacgcgc tcgcgaccct ggtctcgccg gcgatcctga
     2821 tcgtcacgct cgtcggcggc ttcttcatgc tcgcccgcct gcagaccggc gccggcggcg
     2881 cggtggcacg cctgtccgag cgaccgaaga cgcagttcgc cgacgccatc ggcaatgacg
     2941 aggccaaggc cgcgctgcag cgcgtgaagg cgttcctcca cgacccgtcg gcctacgcga
     3001 agctcggcgc cgcggcgccg cgcggcgtgc tgctggtcgg ccccccgggt accggcaaga
     3061 cgctgctcgc caaggcgctg gccggcgaga gccaggccaa cttcatcgcc gtcgacggct
     3121 cgtacttcac cgcgacctac tacggcgcgg gcgtcgccaa ggtcaaggcc ttgttcaagc
     3181 aggcgcgcaa gagcgccccg tgcgtcctgt tcatcgacga ggtcgacggc atcggcaagc
     3241 gctcgcgcaa cgcggagcac ggcggcgaga acgagatgaa ccgcatcatc aaccgggtgc
     3301 tggtcgagat ggacggcttc gagccgatgg acaacgtcgt cgtcgtcgcg gcgaccaacc
     3361 acgagaacaa catcgacgag gcgatgcgcc gtcccggccg cttcgacatg ctggtgcgcc
     3421 tggccaagcc cacgctgccc gagcgcgcga agctgttcga cctgtacctc ggcaaggtgg
     3481 tgcacgacgg ctcggccgac acggcctcgc tcgcgcgcat gacgccgggc gtctcgccgg
     3541 cagaaatcgc caacatcgtc aacaaggccg cctccagcgc ggcggagcgg ggcgccgaga
     3601 aggtctcggc cgaccacgtg ctgcgcgcga tcgagaccca cctgctgggc ggcgaggtct
     3661 cgccggtgaa ggacctgctc accgaggcca cgcgcgtgcg cctcgcgtac cacgaggccg
     3721 gccatgcgct ggtcggccac tggaccggcg ccggcctcgt cgagcgcgtg accatcgagc
     3781 cgcgcggccc cgcgctgggc gtgacctaca tgacgcgcga caccgaggat ccgctgtatc
     3841 gccagggcga gctcgtgagc cgcctcgcga tgatgctggg tggacgcgaa gccgagctgc
     3901 tggtgcaggg caacgtctcc agcggcgcct cggacgacct gaagcgcgcc tccgagctcg
     3961 cgatcgagat ggtgggctcg ctcggcttct cggagacctt cggcctgctg agcgtgggcg
     4021 gcatccccga caagctgctc ggccccgacg tccaggcggc gctgctgaag gaagcgcgca
     4081 cgctgctcga ggacgcgcag ggcacctgcc gccgcatcct caacgcgcag cgcgcccgcc
     4141 tcgacgcgat ggcgcatgcg ctgctgaact gcgaggtgct cgccggcgag gcgctgaaga
     4201 cgatgctggg cgacgtccat gccgtggagg acgcggtggt cgtcgcgccg aagcccgccc
     4261 cggccctgcc ggcaccggcg gcggagctcg agacggcctg acgccgccag gcctcttgcg
     4321 aacgcgccgc cacgccggcg cgttcgcgct tctagaatcc ggcacttctt gcagcgaggt
     4381 gcacggtctt gggaatgcag cggatccggc gggtgctcgc ggcgtgcgtg ccgttcgcgg
     4441 ccatcgtggc ggtgttcgcc gcggccgtgg cctggcacct gggcgagccg cagcggcagg
     4501 ttgatcgctt ccaccggctc ctcgccgacg acgccccgct cgcgcgcgac tacggcgacg
     4561 atgcgcgcgt cgtgctcacg cgccgcggct gcgcgcgcca gtgcagtgcc tacaccgtga
     4621 gcatcaccgg cgccggcgtc gtcgagttcg ccggccgtgc cgacgtctgc gcgaccgcgc
     4681 cgacgaccga ccaggtgccg cgcgggacgg cgcagcggct ggtcgacgcg atggtgcgcg
     4741 ccggcttcga cgatctgccg acgaatggag cgatggcgcg ccgcgggccg ggcgagcagg
     4801 gctccgtcgt catcctcgag gtgggcggcc gccgccacgc cgtcgacgac cgccagctgc
     4861 cggagcgccc gctgctcgcc gcgatcgagc agcaggtc
//
LOCUS       DASVLN010000664         6798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_409333, whole genome shotgun sequence.
ACCESSION   DASVLN010000664 DASVLN010000000
VERSION     DASVLN010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409333"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF453_15730"
     CDS             complement(<1..345)
                     /locus_tag="VF453_15730"
                     /inference="COORDINATES: protein motif:HMM:NF017409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEX7689165.1"
                     /translation="MPSSIELNIGHVPSPRRARNGGEDGDDAPFRIVVLGDFSGRPAM
                     ERPALAQRRGVRVDIDNLDAVFAKFAPVAAIAGLQDAAGAPVRIGLDAIDDFSADAVL
                     ARLPAEAMARLAA"
     gene            complement(450..992)
                     /locus_tag="VF453_15735"
     CDS             complement(450..992)
                     /locus_tag="VF453_15735"
                     /inference="COORDINATES: protein motif:HMM:NF024894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HEX7689166.1"
                     /translation="MSASSLPMPPAIQTRLWQEAGDHPAYAILDGAQNELLVGELHAA
                     GAPPWRCLFMGELEPDMAVVAPYLVELEYSSAFTRRLLAEGWGQNRGVFLTSETPLAA
                     LWRHLRAQVMVYGPNLDPMYFRYYDPRVLRSFLPTCTPRQLAEFFGPVGFFIAEDEEP
                     VRAHAWSLADGQLVAQVVGP"
     gene            complement(1006..1395)
                     /locus_tag="VF453_15740"
     CDS             complement(1006..1395)
                     /locus_tag="VF453_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HEX7689167.1"
                     /translation="MPNHVCNGAVLKCTMGAAPSSMVVLPLHRTLTSNQPAANINDHI
                     PLVNIMPFGVCQSILNPATAAATAAALGVLTPGPCLPNTPAPWVPGSPTVILDNQPAL
                     NKSSMLTCVSGGMISVEFEGQATHQIP"
     gene            complement(1496..3586)
                     /gene="tssI"
                     /locus_tag="VF453_15745"
     CDS             complement(1496..3586)
                     /gene="tssI"
                     /locus_tag="VF453_15745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEX7689168.1"
                     /translation="MARMLDIMTKLGGGTFLVQRFSGREELGRMFEYQLELLSERADI
                     TAEQLLGSDATVSLELDDGTSQRYFNGYVTRFAVQGSVRTTAYKSNVGYLYLMTLNPG
                     LWFQTRSSNSQIFAGTSIGDVIKKQLAANTLNTVDPQIGTTETRDYIVQYRETDFNFI
                     SRLAEHAGIYYYFTHDNGTHTMVLVDEAGKHKPNPIKAQVSFSGEDRGDATLHGLRML
                     SEVQSGSFATGDYNYVTPNTQIAAVDNNPKSHDNAGFEIFDFPIDAVTTAQATTYAKT
                     RYEELTCRYAVVHGSGTERGIQVGFKTTVAEHSVAALNQEYLVISHSFVATNNLAQSS
                     AGGGASFECQFEAIPAGTQFRPARVVPRPTIAGTQPALVVDDFGGDVTDTSNNVKVGA
                     KIARVKVEFYWDRYGKQSCWARVSSAWAGKGYGWQNMPRKGEEVLVAFMEGDPDRPVV
                     VGRVYNAENMPPFELPAHKSITGMKTLSLASDGSSVAGKWNELRFDDQDGSEQIYIQA
                     QKDYDRRVLNDSKTWIGNESHNYVKADTFDKYDADHHTQTTGDRNEKIGGTWSMNVTG
                     DQQVKVGKNLGADVGTEVYIKAGQTVVIEAGMKLTLKCGGNTIAMSPSGIEIAGAPLV
                     KVNSGASGAATGSPVSPTAPKDAKLAMTSEGGTPLTAPTPPTAPTAFSPTASSFKLAA
                     VTAAPFVSAAACEG"
     gene            complement(3671..4396)
                     /locus_tag="VF453_15750"
     CDS             complement(3671..4396)
                     /locus_tag="VF453_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689169.1"
                     /translation="MTPRSTLRHALARLLAVAGLGLAAAVPARAGDEREFAGYDAFYA
                     TLPDALFTAADKRSFGHEAFVDGHGGMTRRWAGEVDGRRHRVAVTGSLLEVDGRAFGQ
                     AHAVVFPRVLPADMGLDARLYVTARDVCVDGVAPTASGTSQRWRYVHFVQDAFGPHAR
                     RYELPGLFGSCLSLQRAAGGDVRFFEASLHTPDGADFPDSAVLQPWRLHRGRFVQDDG
                     PALRAVFKAADDVYDFRIEAPAS"
     gene            complement(4393..5247)
                     /locus_tag="VF453_15755"
     CDS             complement(4393..5247)
                     /locus_tag="VF453_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689170.1"
                     /translation="MATTRMAGPAGWPPSAASAPQQANLMLMGRGDHADPTVTSAGGD
                     TPGTAGINDHSDRNAPRASERPVPAPGAVVQSGGKVVAVGSKVATIDRQTRRLAALAY
                     GEAGVKDDADEIAAIAWAVANRCRAYDQKTVDQILTVDPHYAYAATNPNARYKKLMAA
                     SADEIAADTGMATALKAATDALANTGRDPSNGAFWWDGKDFGTNPNHPKRLQSFHYGD
                     PSHNLFKVDEVADETTLYWKDKNGHDTKKVRGTYKYVWVTTAAYGDTIFWKYDPDFIR
                     ATANKTYR"
     gene            complement(5578..6405)
                     /locus_tag="VF453_15760"
     CDS             complement(5578..6405)
                     /locus_tag="VF453_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX7689171.1"
                     /translation="MATLSTSAPTCEMASRTDPGRVRSANEDAVRFDAGLALAILADG
                     MGGYAAGEVASRMAVDEIFDELASALPHLHAEPVSAERTGLHEDMRFAASRANTSIIA
                     AARREPDYEGMGSTLVVAVLMHGVMTVAHLGDSRAYRFRAGSLEQLTRDHCWVDEQIA
                     IGALSVDAILDTRYRNIVTRALGIEDEIELEVHDHEAQLGDIFLLCSDGLTDMLDDAK
                     IAEHLAVPRPLGDTASALVDAANEAGGRDNVSALLVQVGAPQPAGWAARLVRRMTGG"
     gene            complement(6407..>6798)
                     /locus_tag="VF453_15765"
     CDS             complement(6407..>6798)
                     /locus_tag="VF453_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689172.1"
                     /translation="GPADGMLLASAGPSTRPAEIVVPSPPTTRSTAAVVSGPAHTPMA
                     RMAESRVPVDELYARAQAMEVQLQVPQAVDLYREASQRGHALSSRRLMELYADGAPGV
                     ARDYRVAVFFKERAIQQGATFEPTWRR"
BASE COUNT          891 a         2296 c         2540 g         1071 t
ORIGIN      
        1 cgcggccagg cgcgccatcg cctcggcggg caggcgcgcg aggacggcgt cggcggagaa
       61 gtcgtcgatc gcgtcgagcc cgatgcgcac gggcgcgccg gcggcgtcct gcaggccggc
      121 gatcgccgcg acgggggcga acttcgcgaa cacggcgtcg aggttgtcga tgtcgacgcg
      181 cacgccgcgc cgctgcgcca gcgcggggcg ctccatcgcg ggacggcccg agaagtcgcc
      241 cagcacgacg atgcggaatg gcgcgtcgtc gccgtcctcg ccgccattgc gcgcgcggcg
      301 cggggacggc acgtggccga tgttcagctc gatggaggac ggcatcgcgg gcgctctttc
      361 atggcaggcc gccgcggtcg cgggcggcgg tgggtggagg tcgcagcggg gacttcgaca
      421 cgtcgggggg ttccgggcgc cggcccgcgc tacgggccca ccacctgcgc gacgagctgg
      481 ccgtcggcga gcgaccaggc gtgcgcgcgc acgggctcct cgtcctcggc gatgaagaag
      541 ccgacggggc cgaagaactc cgccagctgc ctgggcgtgc aggtgggcag gaagctgcgc
      601 agcacgcgcg gatcgtagta gcggaagtac atcgggtcga ggttcggccc gtagaccatc
      661 acctgcgcgc gcaggtggcg ccacagcgcg gccagcggcg tctcgctggt gaggaagacg
      721 ccgcggttct ggccccagcc ctcggcgagc aggcggcgcg tgaacgccga gctgtactcc
      781 agctccacga ggtagggcgc gacgacggcc atgtcgggct cgagctcgcc catgaacagg
      841 caccgccacg gcggcgcgcc ggcggcgtgc agctcgccga ccagcagctc gttctgggcg
      901 ccgtcgagga tcgcgtaggc cgggtggtcg ccggcctcct gccagagtcg ggtctggatg
      961 gcgggcggca tcgggaggga ggaagcggac attggggacg ggacgtcagg ggatctggtg
     1021 ggtcgcctgg ccttcgaact cgacggagat catgcccccg gagacgcagg tcagcatcga
     1081 actcttgttc aatgccggct gattgtccag tatgaccgtc ggcgatcccg gtacccacgg
     1141 agccggcgtg ttgggcaggc aggggccggg ggtgagcacg cccagggcgg ccgcggtcgc
     1201 ggcggcggtg gccgggttga ggatggactg gcagaccccg aacggcatga tgttgacgag
     1261 cgggatgtgg tcgttgatgt tggccgccgg ctggttggac gtgagcgtgc ggtgcagggg
     1321 cagcaccacc atcgagctcg gggcggcgcc catcgtgcac ttcagcaccg caccgttgca
     1381 gacgtggttg ggcatggcaa ttctccgggt gaaggccccg cggggcgcct ggacggcgcc
     1441 gccgcggggc cgatggacga cgatcgcgag acgctgcgcg cgcgggccgt gtcgctcagc
     1501 cttcgcaggc cgcggccgac acgaagggcg cggccgtcac ggcggcaagc ttgaagctgc
     1561 tggccgtcgg cgagaacgcg gtgggcgccg tcggcggcgt gggcgcggtc agcggcgtgc
     1621 cgccttcgct ggtcatcgcg agcttggcgt ccttgggcgc ggtcggactg accggcgagc
     1681 cggtcgcggc gccgctcgcg ccggagttca ccttcaccag cggcgcgccg gcgatctcga
     1741 tgcccgaagg cgacatcgcg atcgtgttgc cgccgcactt gagcgtcagc ttcatgccgg
     1801 cctcgatcac gacggtctga ccggccttga tgtagacctc cgtgccgacg tccgcgccga
     1861 ggttcttgcc gaccttcacc tgctggtcgc cggtcacgtt catcgaccac gtgccgccga
     1921 tcttctcgtt gcggtcgccc gtggtctgcg tgtggtggtc ggcgtcgtac ttgtcgaagg
     1981 tgtcggcctt gacgtagttg tggctctcgt tgccgatcca ggtcttggag tcgttgagga
     2041 cgcggcggtc gtagtccttc tgcgcctgga tgtagatctg ctccgagccg tcctggtcgt
     2101 cgaagcgcag ctcgttccac ttgccggcga cgctcgagcc gtcggaggcc agcgacagcg
     2161 tcttcatgcc cgtgatggac ttgtgcgccg gcagctcgaa cgggggcatg ttctcggcgt
     2221 tgtagacgcg gccgacgacg acggggcggt ccgggtcgcc ctccatgaac gcgaccagca
     2281 cctcctcgcc cttgcgcggc atgttctgcc agccatagcc cttgccggcc caggccgagg
     2341 acacgcgcgc ccagcacgac tgcttgccgt agcgatccca gtagaactcg accttcacgc
     2401 gcgcgatctt cgcgcccacc ttgacgttgt tggacgtatc cgtgacgtcg ccgccgaagt
     2461 cgtcgacgac cagcgccggc tgcgtgcccg cgatcgtcgg ccgcggcacg acgcgcgccg
     2521 ggcggaactg cgtgccggcg gggatggcct cgaactggca ctcgaagctc gcgccgccgc
     2581 ccgcggagct ctgcgcgagg ttgttggtcg cgacgaacga gtgcgagatg accaggtact
     2641 cctggttcag cgcggccacc gagtgctccg ccaccgtcgt cttgaagccg acctggatgc
     2701 cgcgctccgt gccgctgccg tggacgacgg cgtagcggca ggtcagctcc tcgtagcgcg
     2761 tcttggcgta ggtcgtcgcc tgcgccgtcg tgaccgcgtc gatcgggaag tcgaagatct
     2821 cgaagccggc gttgtcgtgc gacttcggat tgttgtcgac cgccgcgatc tgcgtgttcg
     2881 gcgtcacgta gttgtagtcg ccggtggcga agctgccgct ctgcacctcg gacagcatgc
     2941 gcaggccgtg cagcgtcgcg tcgccgcgat cctcgcccga gaagctcacc tgggccttga
     3001 tggggttggg cttgtgcttg ccagcctcgt cgaccagcac catcgtgtgg gtgccgttgt
     3061 cgtgggtgaa gtagtagtag atgccggcgt gctcggcgag gcggctgatg aagttgaagt
     3121 cggtctcgcg gtactggacg atgtagtcgc gcgtctcggt cgtgccgatc tgcgggtcga
     3181 cggtgttgag cgtgttggcg gcgagctgct tcttgatgac gtcgccgatc gacgtgcctg
     3241 cgaagatctg cgagttcgac gagcgcgtct ggaaccacag gcccgggttg agcgtcatca
     3301 ggtacaggta gccgacgttg ctcttgtagg ccgtcgtgcg cacgctgccc tggacggcga
     3361 agcgcgtgac gtagccgttg aagtagcgct gcgacgtgcc gtcgtccagc tccagcgaca
     3421 ccgtggcgtc gctgcccagc agttgctccg ccgtgatgtc ggcgcgttcg gacagcagct
     3481 cgagctggta ctcgaacatg cggcccagct cctcgcgccc gctgaagcgt tgcacgagga
     3541 aggtgccgcc gcccagcttg gtcatgatgt ccagcatgcg agccatgtca gttctccttg
     3601 ttggcgggcg cgccgtcggg gcggcccgga tgctcgggtc gtgaatcgtg tcggaagcgg
     3661 gcgcgcgcgt tcatgacgcg ggcgcctcga tccggaagtc gtagacgtcg tcggcggcct
     3721 tgaagaccgc gcgcagggcc gggccgtcgt cctggacgaa gcggccgcgg tgcaggcgcc
     3781 agggctgcag gacggcactg tcggggaagt ccgcgccgtc gggcgtgtgc aggctggcct
     3841 cgaagaagcg gacgtcgccg ccggccgcgc gctgcagcga caggcaggag ccgaacaggc
     3901 ccggcagctc gtagcggcgc gcgtgcgggc cgaacgcgtc ctgcacgaag tggacgtagc
     3961 gccagcgctg cgacgtgccg ctggccgtcg gcgcgacgcc gtcgacgcag acgtcgcgcg
     4021 ccgtcacgta caggcgcgcg tcgaggccca tgtcggcggg cagcacgcgc gggaagacga
     4081 cggcgtgcgc ctgcccgaac gcgcggccgt cgacctcgag caagctgccg gtcaccgcga
     4141 cgcgatggcg ccggccgtcg acctcgccgg cccagcgccg cgtcatgccg ccatggccgt
     4201 cgacgaacgc ctcgtggccg aacgaccgct tgtccgccgc cgtgaacagc gcgtcgggca
     4261 gggtcgcgta gaaggcgtcg tagcccgcga actcgcgctc gtcgccggcg cgcgccggga
     4321 ccgcggcggc cagccccagt ccggcgaccg cgagcaggcg cgcgagcgca tggcggagag
     4381 tggagcgagg cgtcatcggt aggtcttgtt ggccgtcgcc cggatgaagt cgggatcgta
     4441 cttccagaag atcgtgtcgc cgtaggccgc ggtcgtcacc cagacgtact tgtacgtgcc
     4501 gcgcaccttc ttcgtgtcgt gcccgttctt gtccttccag tacagggtcg tctcgtcggc
     4561 gacctcgtcg accttgaaga ggttgtgcga cggatcgccg tagtggaagc tctgcagccg
     4621 cttcggatgg ttcgggttcg tgccgaagtc cttgccgtcc caccagaacg cgccgttgga
     4681 cgggtcgcgg ccggtgttcg cgagcgcgtc ggtggcggcc ttcagggcgg tcgccatgcc
     4741 ggtgtcggcc gcgatttcgt cggcgctcgc ggccatcagc ttcttgtagc gcgcgttcgg
     4801 attggtcgcg gcataggcgt agtggggatc gacggtcagg atctggtcga ccgtcttctg
     4861 gtcgtaggcg cggcagcggt tggccaccgc ccaggcgatc gcggcgatct cgtcggcgtc
     4921 gtccttgacg cccgcctcgc cgtaggccag cgcggccagg cgccgcgtct ggcggtcgat
     4981 cgtcgcgacc ttgctgccca ccgccaccac cttgccgccg gactgcacca ccgcgcccgg
     5041 cgcgggcacg gggcgctcgc tcgcgcgcgg ggcgttgcgg tcgctgtggt cgttgatgcc
     5101 ggccgtgccg ggcgtgtcgc cgccggcgct ggtcacggtc ggatcggcgt ggtcgccacg
     5161 ccccatgagc atgaggttgg cctgctgcgg cgcgctcgcg gctgaaggcg gccagcccgc
     5221 gggtccggcc attctcgtcg tcgccatgga tgtcaaccgc cggtagtcga gcccggctcc
     5281 atcgggagcc cggcaggatc gtcaacgaca caactccggc atcccgaggc cgcgccggcg
     5341 aggcgcggca ggccgaagcg ccggagacct gctccccgac tggcgtcctg cgatcgattc
     5401 agattgcaac acagcccgca ccatcggagg cggcggactt ccctgatccg ccgcgcacgc
     5461 gacgggcggg cgacagtccc gcactttagc gcagagccgc cctcgcggcg catccgggcc
     5521 acccccctgt taggggatgc gaagcgggat tattcgacga tccgccgggc cgcgcggtca
     5581 gccgccggtc atgcgccgca cgaggcgcgc cgcccagccc gcgggctgcg gcgcgcccac
     5641 ctgcacgagc agcgcggaga cgttgtcgcg cccgccggcc tcgttggcgg catccaccag
     5701 cgcggacgcc gtgtcgccga gcggccgcgg gaccgccagg tgctccgcga tcttcgcgtc
     5761 gtcgagcatg tcggtgaggc cgtccgagca cagcaggaag atgtcgccga gctgcgcctc
     5821 gtggtcgtgc acctcgagct cgatctcgtc ctcgatgccc agcgcgcgcg tcacgatgtt
     5881 gcggtaccgg gtatcgagga tcgcgtcgac cgacagcgca ccgatcgcga tctgctcgtc
     5941 gacccagcag tggtcgcgcg tgagctgctc gaggctgccg gcgcggaagc ggtacgcgcg
     6001 cgagtcgccg aggtgggcga ccgtcatgac gccgtgcatc agcaccgcga ccacgagcgt
     6061 cgagcccatg ccttcgtagt ccggctcgcg gcgggcggcg gcgatgatgc tcgtgttcgc
     6121 gcggctcgcg gcaaagcgca tgtcctcgtg caggccggtg cgctcggcgc tcacgggctc
     6181 ggcgtgcagg tgcggcagcg cggaggcgag ctcgtcgaag atctcgtcga cggccatgcg
     6241 gctcgcgacc tcgccggccg catagccgcc catgccatcg gcgaggatcg cgagcgcgag
     6301 cccggcgtcg aagcgcacgg cgtcctcgtt ggcgctgcgc acgcgtccgg ggtcggtgcg
     6361 cgaggccatc tcgcaggtcg gggccgaggt gctcagggtc gccatgtcaa cgtcgccagg
     6421 tcggttcgaa cgtggcccct tgctggatcg cgcgctcctt gaagaacacg gcgacccgat
     6481 agtcgcgcgc gacgccgggg gctccgtcgg cgtagagctc catcagtcgg cgcgaggaca
     6541 gcgcgtggcc gcgctggctc gcttcgcggt acaggtcgac cgcctgcggc acctgaagct
     6601 gcacctccat cgcctgcgcg cgcgcgtaga gttcgtcgac cggcacgcgc gactcggcca
     6661 tgcgcgccat cggcgtgtgc gcggggccac tcacgacggc ggcggtcgag cgcgtcgtcg
     6721 gcggcgaggg cacgacgatc tccgcgggcc gcgtcgacgg gccggccgac gcgagcagca
     6781 tgccgtccgc cggccccg
//
LOCUS       DASVLN010000665         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_195199, whole genome shotgun sequence.
ACCESSION   DASVLN010000665 DASVLN010000000
VERSION     DASVLN010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195199"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1493)
                     /locus_tag="VF453_15770"
     CDS             complement(243..1493)
                     /locus_tag="VF453_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7689173.1"
                     /translation="MLRLRTSPSSPAASKGRAARPRRGDDAFMSGVRAAQVVEGTPPV
                     AWVLYLIAAALVAALTWASFARVDEVTKADCRVVPVGREQVIASLEGGILSELHVHEG
                     EQVAAGQPLAELDPTRVEAQQRESEAKRTALRAAVARLTAEAYGRALTFPREIAGSQA
                     ARDEGDSYAARKHALDEAVASNARDIAMMRRELSVSEQMSAKGLLSEVEVLHLRRQIN
                     EMSLQSEDRVNRFRQDAASDLARSQTELATLDEQMAGRQDVLRRTTLKSPVHGLVKNI
                     RNNTLGGIVGPGAPIMEIVPIGDRTLVEAHVKPSEIGFLQVGQTAKIKLLSYDYTIYG
                     GLEGVIDEISPDAIGDAEKPGGDSTYYRVMLHVQSNTLHEKGKPLPVLPGMTGSAEVR
                     IGERSVLNFLLRPMIKSKEAFRER"
BASE COUNT          195 a          552 c          600 g          239 t
ORIGIN      
        1 cgccgaggtc tgcgcgggcg cgcacgccgc cgcggccagc gcgagcgcca gcgcgatggt
       61 cgcgggccgg cgcgggatga ggttcacgga tgccatgggg tcgaagctcc ttgcggggcc
      121 gcgtgcgtcg cgtcgggcgc agcggcggtt cctgcaagta gaccgtggga aggcggcgcg
      181 cgaaggtcgg aggacgcggg caatcgcggc gcatttcggc gcgcccggcg cggtgccgcg
      241 cgtcaacgct cgcggaacgc ttccttcgac ttgatcatcg gccgcagcag gaagttgagc
      301 accgagcgct cgccgatgcg cacctccgcg ctgccggtca tgccgggcag caccggcagc
      361 ggcttgccct tctcgtgcag ggtgttgctc tgcacgtgca gcatgacgcg gtagtaggtc
      421 gagtcgccgc cgggcttctc ggcgtcgccg atcgcatcgg ggctgatctc gtcgatcacg
      481 ccctcgaggc cgccgtagat cgtgtagtcg taggacagca gcttgatctt ggcggtctgg
      541 ccgacctgca ggaagccgat ctcgctgggc ttgacgtgtg cctcgaccag cgtgcggtcg
      601 ccgatgggca cgatctccat gatcggcgct cccggcccga cgataccgcc caaggtattg
      661 ttgcggatgt tcttcacgag cccgtgcacc ggcgacttca gggtcgtgcg gcgcaggacg
      721 tcctggcggc cggccatctg ctcgtccagc gtcgcgagct cggtctgcga cctggccaga
      781 tcgctcgcgg cgtcctggcg gaagcggttg acgcggtcct cgctctgcag gctcatctcg
      841 ttgatctggc gccgcaggtg cagcacctcg acctccgaca gcaggccctt ggccgacatc
      901 tgctcggaga ccgagagctc gcgccgcatc atcgcgatgt cgcgcgcgtt ggaagccacc
      961 gcctcgtcca gcgcgtgctt gcgcgcggcg taggagtcgc cctcgtccct ggcggcctgg
     1021 gagcccgcga tctcgcgcgg gaaggtgagc gcgcggccgt aggcctcggc ggtgaggcgt
     1081 gcgacggcgg cgcgcagcgc ggtgcgcttg gcctcgctct cgcgctgctg cgcctcgacg
     1141 cgcgtcgggt cgagctcggc gagcggctgg cccgccgcga cctgctcgcc ctcgtgcacg
     1201 tggagctcgc tcaggatgcc gccctccagg ctggcgatga cctgctcgcg gccgacgggc
     1261 acgacgcggc agtcggcctt ggtgacctcg tcgacgcgcg cgaacgacgc ccaggtgagc
     1321 gcggcgacga gcgccgcggc gatcaaatac aggacccagg cgaccggcgg cgtgccctcg
     1381 acgacctgag ccgcgcgcac gcccgacatg aaggcgtcgt cgccgcggcg cgggcgcgcg
     1441 gcgcggccct tggacgcggc gggggacgac ggggacgtgc gcaggcgcag catggactcg
     1501 cgggcttgcc gggatcgatc aggcggccat cgccgccgcc tgggcctgcg gcatgccggg
     1561 ttgcgcgacg gacggcgtgc cctgcg
//
LOCUS       DASVLN010000666         4296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_195207, whole genome shotgun sequence.
ACCESSION   DASVLN010000666 DASVLN010000000
VERSION     DASVLN010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195207"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..734)
                     /locus_tag="VF453_15775"
     CDS             complement(96..734)
                     /locus_tag="VF453_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689174.1"
                     /translation="MKLTDHCSALDRRTVRRLAAALAGTALLAGCGGGGDPLSNPPNV
                     SNPAGAGGDKLSFAYFQKCIAPIFLEPLPIVQNGQSSTNTCAGSGCHASDTGTGGALR
                     IIVSAQPVDLTDPANTPDVIRTTDMYKNFYSAQGVTIPGSPIQSRLFDKPLLLNVLHG
                     GGRIFASQDDPHAQLISYWISHPMPSGQDEFSAAANAMFTPADPATGTCNTQ"
     gene            complement(760..3783)
                     /locus_tag="VF453_15780"
     CDS             complement(760..3783)
                     /locus_tag="VF453_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689175.1"
                     /translation="MKLPIASPARALAASAALLAGAGVLALSACGGGSSHNTDASTVT
                     VQGDVPIAYVKRVRTLGLQQNPTNGAPSLPGGDLMIREKASPSAPEHNITAQFTMGKG
                     DASSPEVSYDGKKLLFAMKCPASNAVTINGGPACTGRWNIWEYDMTTGGLAGGTFRRL
                     TSSTNDDDVDPVYLPAGGGVVFTSNRQTESHANQALGHSYYALDEYERERVFNLHTMA
                     LDGSKITQISFNQSHDRSPTIRPNGQIMFSRWDHVGGRNHFKIFTVNPDGTDMFVLYG
                     AHSDGNSFLHPRDMDPAGKYAGFLTSDLMPLQRTQQGGALMFIDAANYSEQNTPANSS
                     VPATGGQVQATAQELNIERGVSRYGRVTTPYPLWDGTNRVLLAYRPCQVTKNGTITSC
                     ADLTDAELARVSDMQRSIADVQADPIQDNVPPPYAIYMFDPANQSFLNVAAPPDGFMY
                     TNPVPLLARAEPATSAPAPLDPDLAAKGLGTLDVRSVYDTDGLGRMGDPMLTAADLGA
                     GCTAAIAKTTPTDANDTRPQVADIARIKDPTDAAYGCAPARFIRAVRAVAPPSNMMKL
                     RSSLGETDFEAQQVIGYAPIEPDGSFKLNVPADTPIALAVIDAQGRAFQTHTNWIQVR
                     PGEHRTCDGCHSPRRGAALNSGAVVNALSAGLLSSMASAHQSGETMADTRTRLDPSAL
                     TLKPDPVYTDVWADTTKAGVTARPSISLLYTGNPNPADDLATPAPVNGIINYPTHIQP
                     LWDRNRGANTCTNCHNVSDHLDLSHTTGGDGRVISYDRLMIGDPELDASGRPVIFFDD
                     GVPMLSRQAALVNTAASEGEATGLARKSRLIEILSGQSLMSDADAKTAHPNPPASAPD
                     HSKMMNKAEMRLLAEWIDTGGKYFNDPFDPAGGVRMVPTLSEDTFTATVYPIINTTCA
                     GCHAAVGSTNTPTGKTSFTDNRFVLTGDPDGDFGVTLTMISDTCHPPLDYLLAKPSTI
                     PHPDGATSQTTAVLPSTSANYATIATWISSGCTTP"
     gene            complement(3829..>4296)
                     /locus_tag="VF453_15785"
     CDS             complement(3829..>4296)
                     /locus_tag="VF453_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX7689176.1"
                     /translation="GFAKGHAVDNAARLLGQLGVRDAMVAAGGDSRVMGTRGNRPWTV
                     AVRDPRREGEVVAVLPLSDVSISTSGDYERFFERDGVRCHHLLDPRTGRSPDGVRSVT
                     IIADDGLTSEAFSKTVFVLGVQDGLRFVEAHPGVDAIVVDAQGALHYSSGLAG"
BASE COUNT          547 a         1384 c         1655 g          710 t
ORIGIN      
        1 ccatgccgcg caggccacga gcaccacggc cgcgagcgtc gcgcgcaggc acgcgggcgc
       61 gccggcgcgg cggacggcga cgacgacggc gccgctcatt gcgtgttgca cgtgcccgtg
      121 gccggatcgg cgggcgtgaa catggcgttc gccgcggcgc tgaactcgtc ctgcccgctg
      181 ggcatcggat ggctgatcca gtacgagatc agctgcgcgt gcgggtcgtc ctggctcgcg
      241 aagatgcggc cgccgccgtg cagcacgttc agcagcagcg gcttgtcgaa caggcggctc
      301 tggatcgggc tgcccgggat cgtcactccc tgcgccgagt agaagttctt gtacatgtcg
      361 gtcgtccgga tcacgtcggg cgtgttggcg gggtcggtga ggtcgaccgg ctgcgccgag
      421 acgatgatcc gcagcgcgcc gccggtgccg gtgtcgctgg cgtggcagcc cgatccggcg
      481 caggtgttcg tcgacgactg gccattctgg acgatgggca ggggctccag gaagatcggc
      541 gcgatgcact tctggaaata ggcgaacgac agcttgtcgc cgcccgcgcc cgcgggattc
      601 gagacgttgg gcgggttgct gagcgggtcg cccccgccgc cgcagcccgc gagcagcgcc
      661 gtcccggcca gggccgccgc cagccggcgg acggttcggc gatccagggc gctgcagtga
      721 tcggtcagct tcaagtccgg ttcctcggtc gtcggggcgt cagggcgtcg tgcagccgct
      781 ggagatccag gtggcgatcg tcgcgtagtt cgcgctggtg gacggcagca ccgcggtcgt
      841 ctgcgaggtc gcgccgtccg ggtgcgggat cgtcgacggc ttggccagca ggtagtccag
      901 cggcggatgg caggtatccg agatcatcgt cagggtcacg ccgaagtcgc catcgggatc
      961 gccggtgagg acgaagcgat tgtccgtgaa cgaggtcttg ccggtgggcg tgtttgtact
     1021 gccgacggcg gcgtggcagc ccgcgcacgt ggtgttgatg atgggataga ccgtcgcggt
     1081 gaacgtgtcc tcgctgagcg tgggcaccat gcgaacgccg ccggccggat cgaacgggtc
     1141 gttgaagtac ttgccgccgg tgtcgatcca ttcggcgagc aggcgcatct ccgccttgtt
     1201 catcatcttc gagtggtcgg gagcgctcgc cggcgggttc ggatgcgcgg tcttcgcgtc
     1261 ggcatccgac atcagggact gccccgacag gatctcgatc agccggctct tgcgcgcgag
     1321 gccggtcgcc tcgccctcgc tggcggcggt gttcacgagc gccgcctgcc gcgacagcat
     1381 cggcacgccg tcgtcgaaga agatcaccgg acggcccgag gcgtcgagct cggggtcgcc
     1441 gatcatcagg cggtcatagg agatcacgcg cccgtcgccg ccggtcgtgt ggctcaggtc
     1501 gaggtggtcc gagacgttgt ggcagttggt gcaggtgttg gcgccgcggt tgcggtccca
     1561 cagcggctgg atgtgcgtcg ggtagttgat gatgccgttg accggcgccg gcgtggcgag
     1621 gtcgtcggcc gggttcggat tgccggtgta cagcagcgag atcgacgggc gcgccgtgac
     1681 gccggccttg gtcgtgtcgg cccagacgtc ggtgtagacc ggatcgggct tgagggtgag
     1741 cgcgctcggg tcgagccggg tgcgggtgtc ggccatggtc tcgccggact ggtgcgcgct
     1801 cgccatcgag gacagcagcc ccgccgacag cgcgttgacc accgcgcccg agttcagcgc
     1861 cgcgccgcgc cgcgggctgt ggcagccgtc gcaggtgcgg tgctcgccgg gccgcacctg
     1921 gatccagttg gtgtgggtct ggaacgcgcg gccctgggcg tcgatcaccg ccagcgcgat
     1981 cggcgtgtcg gccggcacgt tgagcttgaa cgagccgtcc ggctcgatcg gcgcgtaccc
     2041 gatgacctgc tgcgcctcga agtcggtctc gccgagcgag ctgcgcagct tcatcatgtt
     2101 ggacggcggg gcgaccgcgc gcaccgcgcg gatgaagcgc gccggcgcgc agccgtaggc
     2161 cgcgtcggtc ggatccttga tgcgtgcgat gtcggccacc tgcggacgcg tgtcgttggc
     2221 gtcggtgggc gtggtcttgg cgatcgccgc cgtgcagccg gcgcccaggt cggcggcggt
     2281 gagcatcggg tcgcccatgc ggccgaggcc gtcggtgtcg tagacgctgc ggacgtccag
     2341 cgtgcccagg cccttagcgg cgaggtcggg atccagcggc gcgggcgcgc tggtcgccgg
     2401 ctcggcgcgc gcgagcagcg gcaccgggtt ggtgtacatg aagccgtcgg gcggcgcggc
     2461 cacgttcagg aaggactggt tggccggatc gaacatgtag atcgcgtagg gcggcggcac
     2521 gttgtcctgg atcgggtcgg cctggacgtc ggcgatcgag cgctgcatgt cggacacccg
     2581 agcgagctcg gcgtcggtga ggtcggcgca ggacgtgatc gtgccgttct tcgtcacctg
     2641 gcaggggcgg taggccagca gcacgcggtt ggtgccgtcc cacagcgggt acggcgtggt
     2701 gacgcgcccg tagcgcgaga ctccgcgctc gatgttgagc tcctgcgccg tcgcctgcac
     2761 ctggccgccg gtcgcgggca ccgacgagtt cgcgggcgtg ttctgctcgg agtagttcgc
     2821 cgcgtcgatg aacatcagcg cgccgccctg ctgcgtgcgt tgcagcggca tgaggtcgga
     2881 ggtcaggaag ccggcgtact tgccggccgg atccatgtcg cgcgggtgca ggaagctgtt
     2941 gccgtcgctg tgcgcgccgt acagcacgaa catgtcggtg ccgtccgggt tgaccgtgaa
     3001 gatcttgaag tggttgcggc cgccgacgtg atcccagcgc gagaacatga tctggccgtt
     3061 cggccggatc gtcgggctgc ggtcgtggct ctggttgaac gagatctgcg tgatcttgct
     3121 gccgtccagc gccatcgtgt gcaggttgaa cacgcgctcg cgctcgtact cgtcgagcgc
     3181 gtagtagctg tggccgagcg cctggttggc gtgcgactcg gtctggcggt tggacgtgaa
     3241 gaccacgccc ccgccggccg gcaggtagac cgggtcgacg tcgtcgtcgt tcgtcgagga
     3301 ggtcaggcgc cggaacgtgc cgccggccag gccgccggtg gtcatgtcgt attcccagat
     3361 gttccagcgg cccgtgcacg cggggccgcc attgatcgtg acggcgttgg acgccgggca
     3421 cttcatcgcg aacaggagct tcttgccgtc gtacgagacc tcggggctgg acgcgtcgcc
     3481 cttgcccatc gtgaactgcg cggtgatgtt gtgctcgggc gcgctcggcg aggccttctc
     3541 gcggatcatc aggtcgcccc cgggcaggct gggcgcgccg ttggtcgggt tctgctgcag
     3601 gcccagcgtg cgcacgcgct tgacgtaggc gatcggcacg tcgccctgca ccgtgacggt
     3661 cgacgcgtcg gtgttgtgcg agctgccacc gccgcacgcg ctgagcgcga ggacgccggc
     3721 gccggcgagc agcgcggccg atgcggcgag cgcgcgcgcg ggagaggcga tgggcagctt
     3781 catgatgggg ttcctcgcgt tcttgctgtg ttctcgcggc gcgccgcgtc agccggccag
     3841 gccggacgag tagtgcaggg cgccctgcgc gtcgacgacg atcgcgtcca cgccgggatg
     3901 ggcctcgacg aagcgcaggc cgtcctgcac gccgagcacg aagacggtct tggagaacgc
     3961 ctcgctcgtg aggccgtcgt ccgcgatgat cgtcacgctg cgcacgccgt cgggcgagcg
     4021 gccggtgcgc gggtcgagca ggtggtggca gcgcacgccg tcgcgctcga agaagcgctc
     4081 gtagtcgccg gaggtggaga tcgagacgtc cgacaaaggc agcaccgcga cgacctcgcc
     4141 ctcgcgccgc gggtcgcgca ccgccaccgt ccacggccgg ttgccgcgcg tgcccatgac
     4201 gcggctgtcg ccgccggcgg ccaccatcgc gtcgcgcacg cccagctggc ccagcagccg
     4261 cgccgcgttg tcgacggcat ggcccttggc gaagcc
//
LOCUS       DASVLN010000667         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_409370, whole genome shotgun sequence.
ACCESSION   DASVLN010000667 DASVLN010000000
VERSION     DASVLN010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409370"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1619
                     /locus_tag="VF453_15790"
                     /pseudo
     CDS             1..1619
                     /locus_tag="VF453_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405931.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
BASE COUNT          199 a          592 c          616 g          212 t
ORIGIN      
        1 cgagtcgcgc gcccgcggcg tcgagggctt catgctcaag cagctcgacg ggctctacgg
       61 cgcgggccgc acgaaggacg tcggcacgtg gtggaagtgg aagatcgacc cgtacacggt
      121 cgacgcggtg ctggtctacg cgcagggcgg ccatggacgg cgcgcgaacc tgttcacgga
      181 ctacaccttc gcggtctggg acggcgtggc cggcgacgaa tccaatccgc gccgcctggt
      241 gccgttcgcg aaggcctact cgggcctcac cgacgaggag atccgacagg tcgaccgccg
      301 cgtgcgcgac accaccaccg agcgcttcgg gccggtgcgc gccgtgacgc cgacgctcgt
      361 cgtcgaactc ggcttcgagg ggctgcaggc ctcgccgcgc cacaagtcgg gcgtcgccgt
      421 gcgcttcccg cgcatgctgc gcctgcggtg ggacaagccg gtcgaggagg cggactcgct
      481 cgcgacgctg catgccttca tcgcggcggc gcgcggcgcg gctccatgac ggtgtcccgc
      541 gacgacgtgc tcgcctggct cgccggccgc ggctggcagc cgttcgcctt ccagcgcgag
      601 acctgggacg cgatggccgc gggccgcagc gggctgctgc atgccaatac cggtaccggg
      661 aagacgtacg cggtgtggct gggcgcgctg caggcgctcg cgtccggcga cggcgctgcg
      721 cgcacggcgc ccccgctgac ggtgctctgg atcacgccga tgcgcgcgct cgccgccgac
      781 accctgcgtg cgctgcgcga gccgctcgcc acgctcgcgc cgcgctggac gatcggacag
      841 cgcagcggcg acacctcgtc atccgagcgc agcgcgcagg atcgccgcct gccgaccgtg
      901 ctggtgacga cgccggagag cctgacgctg ctgctcgcgc gcgcggacgc accgcaggcg
      961 ctcgcgcacg tgaggatggc ggtggtcgac gagtggcacg agctgctcgg caacaagcgc
     1021 ggcgtgcagg cgcagctggc gctcgcacgc ctgcggcggt tcaatccggc gctggcgagc
     1081 tgggcgctct cggccacgct gggcaacctc ggcgaggcgc tggcgacgct ggtcggcgag
     1141 cccgcgccga tacggggtgc cgtacccggg cccgtgatcg tccgcggcga cgtccccaag
     1201 acgctggtca tcgacaccct gctgcccgcg cgcgccgagc gcttctcgtg ggccgggcac
     1261 ctgggcctgc agatgctgcc gcaggtggtc gccgcgctcg aggaggagag cccggcgctc
     1321 gtcttcacca acacgcgctc gcaggccgag cgctggtacc aggcgctgct ggaggcgcgc
     1381 cccgactggg ccggcgagct cgcgctgcac cacggctcgc tcgcgcgcga ggtgcgcgag
     1441 tgggtcgagg cgcagctgaa cgcgggcgcg ctccgggccg tcgtgtgcac ctccagcctc
     1501 gacctcggcg tcgacttcct gcccgtgcgg cgggtgctgc aggtcggctc ggccaaaggg
     1561 atcgcgcgcc tgctgcagcg agccgggcgc tcggggcatg cgcccgggcg gccgtcgcg
//
LOCUS       DASVLN010000668         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_248768, whole genome shotgun sequence.
ACCESSION   DASVLN010000668 DASVLN010000000
VERSION     DASVLN010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248768"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VF453_15795"
     CDS             <1..339
                     /locus_tag="VF453_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7689177.1"
                     /translation="ARAERAYAALDVVTLLGTADVDPDHPMLDHGCAAEAQGATRFER
                     GHDFVAALEADTGPGFAQAVFDVPGVGHTSHGMFTSTCGVAVLFGVGTCAAPAASAAA
                     SAIAPVAAGR"
     gene            complement(348..1298)
                     /locus_tag="VF453_15800"
     CDS             complement(348..1298)
                     /locus_tag="VF453_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689178.1"
                     /translation="MPKSLLYVPGLAMPELVVKQARTPSEFTAKVSEAFQGDIYMRDM
                     VPVILNKGALPRDCRQSLHWIDIPRAPAAKPGHRRSPAINEMPAPKGGPSKAFKTLGT
                     PATNVLSLQPGVQAAPMKMSVGMVLTPCTAPEYLAQAMPSGAKTKALADVAAGDTLYI
                     VGHSNAQGGSLTYKCPAPGHVVKSDKHPAGCAGWQHAEKRHIDPVTLASLLINEGLPA
                     RVQFDIALVACYSAGLDDAQLQSVQSFAQRLAGALNGRGCRARVYGATGLTSAGTEVQ
                     VARGAQKKPDGTILLDPNTREDLPDGGGQPFYKRFFRFYA"
     gene            complement(1359..>1554)
                     /locus_tag="VF453_15805"
     CDS             complement(1359..>1554)
                     /locus_tag="VF453_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEX7689179.1"
                     /translation="LKITMTEVLVTSVSQGGSGGEDRLTENVTLNFASVKVDYVEQTP
                     KGGTGDKPSMNWNIAENSKD"
BASE COUNT          181 a          521 c          592 g          260 t
ORIGIN      
        1 gcccgggccg agcgggccta cgcggcgctg gacgtcgtca cgctgctcgg caccgccgac
       61 gtcgatccgg accacccgat gctcgatcac ggctgcgccg cggaggcgca gggcgcgacg
      121 cgcttcgagc gcgggcacga cttcgtggcg gcgctggagg cggacacggg accgggcttc
      181 gcgcaggcgg tgttcgacgt gccgggcgtc ggccatacca gccacggcat gttcacgtcg
      241 acgtgcgggg tcgcggtgct gttcggcgtc gggacgtgcg cggcgccggc ggcgtccgcg
      301 gcggcctcgg cgatcgcgcc ggtggccgcc gggcgctgaa gccgcgatca ggcgtagaag
      361 cggaagaacc gcttgtagaa cggctggccg ccgccgtcgg gcaggtcctc gcgggtgttg
      421 ggatcgagca ggatcgtgcc gtccggcttc ttctgggcgc cgcgggcgac ctgcacttcg
      481 gtgccggcgc tggtgaggcc cgtggcgccg tacacccgtg cgcggcagcc gcgtccgttg
      541 agcgcgccgg ccaggcgctg cgcgaacgac tgcacggact gcagttgcgc gtcgtccagg
      601 cccgcgctgt agcaggccac gagcgcgatg tcgaactgca ctcgggcggg cagcccctcg
      661 ttgatcagca ggctggccag ggtcaccggg tcgatgtggc gcttctcggc gtgctgccat
      721 ccggcgcagc cggcggggtg cttgtcgctc ttgacgacgt ggccgggcgc cgggcacttg
      781 taggtgagcg agccgccctg ggcgttggag tgccccacga tgtagagcgt gtcgcccgcc
      841 gcgacgtcgg ccagggcctt ggtcttcgcg ccgctgggca tggcctgggc gaggtactcg
      901 ggcgcggtgc agggcgtgag caccatgccc acgctcatct tcatcggcgc ggcctgcacg
      961 ccgggctgca gcgacagcac gttggtggcc ggcgtgccga gcgtcttgaa cgccttggac
     1021 gggccgccct tgggcgcggg catctcgttg atggccggcg agcggcggtg gcccggcttg
     1081 gcggccggcg cgcgcgggat gtcgatccag tgcaggctct ggcggcagtc gcgcggcagg
     1141 gcgcctttgt tcaggatcac cggcaccatg tcgcgcatgt agatgtcgcc ctggaacgcc
     1201 tcggacacct tcgcggtgaa ctccgagggc gtcctggcct gcttgaccac cagttccggc
     1261 atcgcgaggc cggggacgta gagcaatgac ttgggcacgg tggcctcctc ctctttgtgc
     1321 gggccgcccc gatcggggcc ggcccggctc gtgtggaatc agtccttcga gttctcggcg
     1381 atgttccagt tcatcgacgg cttgtcgccc gttccgccct tcggggtctg ctcgacgtag
     1441 tcgaccttga ccgaggcgaa gttcagcgtg acgttctccg tcaggcggtc ctcgccgccc
     1501 gagccgcctt gcgacaccga ggtcacgagg acttcggtca tcgtgatctt cagg
//
LOCUS       DASVLN010000669         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_248864, whole genome shotgun sequence.
ACCESSION   DASVLN010000669 DASVLN010000000
VERSION     DASVLN010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248864"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..194)
                     /locus_tag="VF453_15810"
     CDS             complement(57..194)
                     /locus_tag="VF453_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689180.1"
                     /translation="MTTKSLLVALLLSTVAVAASAAPHHKHRKHHRHHARHHHVVRHH
                     G"
     gene            complement(334..618)
                     /locus_tag="VF453_15815"
     CDS             complement(334..618)
                     /locus_tag="VF453_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689181.1"
                     /translation="MNKTLTALLAAAGLAFASLAAHAADANSQNDKQATAQYKADKKR
                     ADANEDLNKAKCGSSLTKEGRACKSDASAEAKKEKADAKVNEVNTKDRNK"
     gene            complement(695..1093)
                     /locus_tag="VF453_15820"
     CDS             complement(695..1093)
                     /locus_tag="VF453_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEX7689182.1"
                     /translation="MPEPGWTALADLVLAVHAAIAASVVLGLFAIVVGNLRGWRWVND
                     GRFRAAHLAAIAIVAAESWLGIPCPLTTLETSLRARAGGTTYAGGFIEHWVRRALFFD
                     GPAWAFTLAYTAFGLAVLASWWRWPPRRRG"
     gene            complement(1086..1862)
                     /locus_tag="VF453_15825"
     CDS             complement(1086..1862)
                     /locus_tag="VF453_15825"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HEX7689183.1"
                     /translation="MTLATDPSRATEPAADEHALAWRSPQVAALRADLALALRAAAHH
                     GLAEGVCNHFSVALPDGSDRFLLNPRGLLWSEVGADDVVLVDGDGAALAGRHAVEPTA
                     MFIHAAIHRRGRPCVLHTHMPHATALTLTVDRALDTRLSQNAMRFHGRVGVDDRYNGL
                     ALDVAEGERIADALGAADVVFLGNHGVVVCAAQIAHAYDDLYYLERACMAQVLAQSTG
                     RPLAPAPEALARRVGEQTLGERLQSDLFFEALRRALPERA"
     gene            2038..>2271
                     /locus_tag="VF453_15830"
     CDS             2038..>2271
                     /locus_tag="VF453_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689184.1"
                     /translation="MPTRIASHRLNLVCAALAALALVAGCSKNDSTQTAANEDAASNT
                     AATQPTDQPAADQTAGNQAATNQGAPAQATGARG"
BASE COUNT          289 a          806 c          880 g          296 t
ORIGIN      
        1 ccccgggccc gcgggcgcgc ctccaccggc cgcctcgcgc ggccggcttc gcttcgtcag
       61 ccgtggtggc ggacgacgtg gtggtgcctg gcgtggtggc ggtggtgctt gcggtgcttg
      121 tgatgcggcg ctgccgatgc ggcgacggcg acggtcgaca gcagcagggc gacgagcagg
      181 gacttggtgg tcatggtgtc gtggtgcggg ggagttggcg atgcggggcc tgcggcccgc
      241 gtcgactccc gatcgcacgc gggacgcgac gcggccccgg cgcgattgtc tcgcaccggg
      301 gccgcgtcgc atcgcgggcg tcgcggcgcg cgcttacttg ttgcggtcct tggtgttgac
      361 ctcgttgacc ttggcgtcgg ccttctcctt cttggcctcg gcggacgcgt cggacttgca
      421 ggcgcggcct tccttggtca gcgacgagcc gcacttggcc ttgttcaggt cctcgttggc
      481 gtcggcgcgc ttcttgtcgg ccttgtactg ggcggtcgcc tgcttgtcgt tctgcgagtt
      541 cgcgtcggcg gcgtgggcgg ccaggctggc gaaggcgagg cccgcggcgg cgaggagggc
      601 ggtcagggtc ttgttcatgg tgtgctccgt ggagttgccg ggcgaagccg gtgagccctg
      661 acggcggcaa gggccgtgcc ctcgaccgtg gcgatcagcc ccggcggcgc ggcggccagc
      721 gccaccagct cgcgagcacc gcgagcccga aggccgtgta ggcgagcgtg aacgcccagg
      781 ccggcccgtc gaagaagagc gcacgtcgga cccagtgctc gatgaagccg cccgcgtagg
      841 tcgtgccgcc cgcgcgggcg cgcagcgacg tctccagcgt cgtcagcggg caggggatgc
      901 cgagccagga ctccgcggcc acgatcgcga tcgccgccag gtgcgccgcg cggaaccgcc
      961 cgtcgttgac ccagcgccag ccgcgcaggt tgccgacgac gatcgcgaac aaccccagca
     1021 ccaccgacgc cgcgatcgcc gcatggacgg cgagcacgag gtcggcgagt gcggtccagc
     1081 cgggttcagg cacgctcggg cagcgcgcgg cgcagcgcct cgaagaacag gtccgactgc
     1141 agccgctcgc ccagcgtctg ctcgccgacg cggcgggcaa gcgcctcggg cgcgggagcg
     1201 agcggccggc cggtcgactg cgcgagcacc tgtgccatgc acgcccgctc gaggtagtac
     1261 aggtcgtcgt acgcatgcgc gatctgcgcc gcgcacacga ccacgccgtg gttgccgagg
     1321 aacacgacgt ccgccgcgcc gagggcgtcg gcgatgcgtt cgccctcggc cacgtcgagc
     1381 gccaggccgt tgtagcggtc gtcgacgccg acgcgcccgt ggaagcgcat cgcgttctgc
     1441 gacagccgcg tgtcgagcgc gcggtcgacg gtcagcgtca gcgcggtcgc gtgcggcatg
     1501 tgcgtgtgca gcacgcaggg cctgccacgg cggtggatcg cggcgtggat gaacatggcg
     1561 gtcggctcca ccgcatggcg ccccgcgagc gcggcgccgt cgccgtcgac gagcacgaca
     1621 tcgtcggcgc cgacctcgct ccagagcagg ccgcgcgggt tgagcaggaa gcggtcggag
     1681 ccgtccggca gcgcgacgct gaagtggttg cacacgccct cggccaggcc gtggtgggcg
     1741 gcggcccgca aggcgagggc gaggtccgcg cgcagggcgg cgacctgcgg cgaacgccac
     1801 gcgagggcat gctcgtcagc cgcgggctcg gtggcgcggg aagggtcggt ggcgagggtc
     1861 atggccggat gttaggcccg gacggccccg cgcggcgtgg ctgtcggcag cctgaatcgg
     1921 cggcgcggcg cgggccggcc gctcgaccgc gagtggcggg ccgcccgatt gccgagatgg
     1981 gcgatcccgg cgtgccggcc gttcgacggc acggccccgc acacacggag gaaatccatg
     2041 ccgacccgaa tcgcgtccca ccgcctgaac ctcgtctgcg ccgcgcttgc ggccctcgcg
     2101 ctggtcgccg gctgctcgaa gaacgactcg acccagaccg cggccaacga ggacgccgca
     2161 tcgaacaccg cggccactca acccaccgac cagcccgcgg ccgaccagac ggccggcaac
     2221 caggccgcca ccaaccaggg cgcgccggca caggccaccg gcgcccgggg c
//
LOCUS       DASVLN010000670         7571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_88463, whole genome shotgun sequence.
ACCESSION   DASVLN010000670 DASVLN010000000
VERSION     DASVLN010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7571
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88463"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VF453_15835"
     CDS             <1..477
                     /locus_tag="VF453_15835"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEX7689185.1"
                     /translation="VARPGVQASVVLGEALGARSPVETSSPMFLVDVRLEPGARIELD
                     VAHAERGAYVIEGEVHDESNPGATVPKEHLVVFEPGEPAVLVAGAAAPAHVTFLGGPP
                     LDAPRFLWWNFVSSSRDRVLEAQAAWSRQEFPPVPGETEYIPLPSSRPLPPAGSAL"
     gene            485..1165
                     /locus_tag="VF453_15840"
     CDS             485..1165
                     /locus_tag="VF453_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX7689186.1"
                     /translation="MYGHALALALQHGHALFLFLELAGTFAFATSGAVAARERGLDGF
                     GILVIAFVVACGGGVLRDLCLGAVPPAGLSDWRYLAVALAAAGLAMAAHGVVERLAHP
                     VVLFDSLGLGLFAVAGAQKALLLGAGDEAAVMLGGVTAVGGGVLRDVLLNRVPVILRR
                     EIYALAALVGAGIETAGTALGASSAGLTVGAVASCFALRWLSLRFGWNLPRFRRPGAH
                     EGLDSMVE"
     gene            1243..1956
                     /locus_tag="VF453_15845"
     CDS             1243..1956
                     /locus_tag="VF453_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX7689187.1"
                     /translation="MTAFDSLSDFPITAKWPARHPDRLQLYSLPTPNGVKVSIALEET
                     GLPYEPHLVRFDANEQMSPEFLSLNPNNKIPAIIDPNGPGGRPLPLFESGAILVYLAG
                     KTGQLMPADDAGRYEVLQWLMFQMGGIGPMFGQVGFFHKFAGRDFEDKRPRDRYVAEA
                     RRLLAVLDGHLDGREWIVGDRYSIADIASFPWINNLVGFYNARELVGFDDFANVKRVL
                     AAFLERPAVQRGLKIPARD"
     gene            complement(2006..2584)
                     /locus_tag="VF453_15850"
     CDS             complement(2006..2584)
                     /locus_tag="VF453_15850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7689188.1"
                     /translation="MPKLSRAAALAAAALVAFGLAGGARAAYVFSNDGGGDGTLVGTE
                     PSFSIFGSNNGDPASADGLPNTAFYVQTFATDAVVAFSWTYETFDSGGAVYDPAGWIL
                     NGVETQLSIDGDPGQGSSGFASVVVRAGDTFGWYVASVDSRYGCGELDIGGGAAGGRC
                     SHMNGPPGVPEPGSLALMTAGLAGLALVARRR"
     gene            complement(2769..3272)
                     /locus_tag="VF453_15855"
     CDS             complement(2769..3272)
                     /locus_tag="VF453_15855"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7689189.1"
                     /translation="MTIRRLCTEDAAAYQALRLAALLDTPEAFASSHEEEKDLPLGEV
                     ARRLVDERDPGVLGAFEAGQLVGVVGLARARFAKMAHKGIVWGMYVAPSARRLGLARQ
                     LMVAALDLARSTAGLAKVTLEVDAANVAAIALYESLGFVVFGRESDAMRIGTDIRNDL
                     QMALALG"
     gene            3368..4474
                     /locus_tag="VF453_15860"
     CDS             3368..4474
                     /locus_tag="VF453_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX7689190.1"
                     /translation="MKTRAAVAWKAGAPLTIETVDLEGPRAGEVLVEVKATGICHTDQ
                     FTLSGADPEGLFPAILGHEGAGVVLEVGAGVTSLRPGDHVIPLYTPECRQCKFCLSRK
                     TNLCQQIRATQGKGLMPDGTSRFSLDGRPILHYMGTSTFANHIVAPEIALAKIRPDAP
                     FDKVCYIGCGVTTGVGAVLFTAKVEAGANVVVFGLGGIGLNVIQGARMVGADRIVGID
                     INPAREAMARRFGMTHFLNPREHANIVDAIVQLTDGGADYSFECIGNVATMRQALECC
                     HKGWGKSVIIGVAGAGQEISTRPFQLVTGREWKGSAFGGARGRTDVPRIVDWYMDGKI
                     SIDPLITHTLELEDINHGFELMERGESIRSVVVY"
     gene            4474..5331
                     /gene="fghA"
                     /locus_tag="VF453_15865"
     CDS             4474..5331
                     /gene="fghA"
                     /locus_tag="VF453_15865"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX7689191.1"
                     /translation="MAGGEIEILSEHACFGGVQGFYRHASATIGLPMRFGVFRPPQAL
                     DGRPVPTLFWLAGLTCNEETFAIKAGAQRVAAELGLMIVTPDTSPRETGVPDADKDWD
                     FGVGAGFYLDATQAPWAMHWRMESWITRELPEVLAARFPWRRERTGIFGHSMGGHGAL
                     TLALRHPGRYASVSAFAPIAAPSQAPWGIKAFTGYLGADRAAWAAHDAEALVASGARA
                     PSLLVDQGQADKFLADQLHVDRFESTCRAAGQPLVLRRHAGYDHGYFFIQSFMEDHLR
                     FHADSLARV"
     gene            complement(5438..5986)
                     /locus_tag="VF453_15870"
     CDS             complement(5438..5986)
                     /locus_tag="VF453_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689192.1"
                     /translation="MGSLELCLEALVLAAAAIAVYEGSRRLLQGGFGRLRVLLLVAGL
                     VPLVGEGVMSLRFATAVSVMLGTEAAMKAEEPPGGWEKQAIPPEERTRKSLEGARINY
                     ALSGRPGTYVDAAGQRVAFVPSADELQRREDLVRARQSALDNGAQFDERGALLLAGAC
                     GLMLCGAVVGGVQRRRARRSGA"
     gene            complement(6131..6346)
                     /locus_tag="VF453_15875"
     CDS             complement(6131..6346)
                     /locus_tag="VF453_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689193.1"
                     /translation="MQISASQASPSAASISNAFPAARLGAMGAMGAMVADSVRVVRAA
                     AMSLARPATLRWAQAQALKIVTVVAGH"
     gene            complement(6487..7458)
                     /locus_tag="VF453_15880"
     CDS             complement(6487..7458)
                     /locus_tag="VF453_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7689194.1"
                     /translation="MSLDPQTARRHAAAYAQRMNRVLDHIDARLDVELDLVTLAAVAH
                     FSPFHFHRVFSAWMGETLGDYLRRRRLDRAALILAGNARRSVLEVALAVGFGSGEAFA
                     RAFRHRFGCTPSAWRRATPQRWQQELAEQRRRLVRREADRRDRKDDQAGGPVASDDGG
                     IPIPEAFMEVTIETLPAARVAYLRHIGPYGKPVAEFWLRVAVPWLQAQGLADGPLYGV
                     GRDDPSVTPPDKCRYDVCCPIPDGVMPAAPAGVDTLPGGRYAVAPFFGDSSDIAHAYT
                     ELLRDWLPASGYGMDDRPIYEYYRPDARYEADTGRFECELCIPLRPA"
BASE COUNT         1039 a         2745 c         2748 g         1039 t
ORIGIN      
        1 gtcgcgcgcc cgggcgtgca ggcgagcgtg gtgctgggcg aggcgctggg cgcgcgctcg
       61 ccggtcgaga cgagctcgcc gatgttcctg gtcgacgtgc ggctggagcc gggcgcgcgc
      121 atcgaactgg acgtcgcgca cgcggaacgc ggcgcgtacg tgatcgaggg cgaggtgcac
      181 gacgagtcga atcccggggc gaccgtcccc aaggagcacc tcgtcgtctt cgagcccggc
      241 gagccggcgg tgctcgtcgc cggcgccgcc gccccggcgc acgtcacctt cctcggcggc
      301 ccgccgctgg atgcgccgcg cttcctgtgg tggaacttcg tctccagcag ccgcgaccgc
      361 gtgctcgagg cgcaggccgc ctggtcgcgc caggaattcc cgcccgtgcc gggcgagacc
      421 gagtacatcc cgctgccgtc ctcgcggccc ctgccgccgg ccgggtcggc gctctgagcc
      481 ggcgatgtac ggccacgcgc tcgcgctcgc cctgcagcac ggccacgcgc tgttcctgtt
      541 cctggagctg gccggcacct tcgcgttcgc gaccagcggg gcggtggcgg cgcgcgagcg
      601 cgggctggac ggcttcggca tcctcgtgat cgcgttcgtc gtggcctgcg gcgggggcgt
      661 gctgcgcgac ctgtgcctcg gcgcggtgcc gccggcggga ctgtccgact ggcgctacct
      721 cgccgtggcc ctggcggccg cggggctggc gatggcggcg cacggcgtcg tcgagcggct
      781 cgcgcatccg gtcgtgctgt tcgactcgct gggcctgggc ctgttcgcgg tcgccggcgc
      841 gcagaaggcg ctgctgctgg gcgccggcga cgaggccgcc gtgatgctgg gcggcgtcac
      901 cgcggtgggc ggcggcgtgc tgcgcgacgt gctgctcaat cgcgtgccgg tgatcctgcg
      961 gcgcgagatc tacgcgctcg cggcgctggt gggcgccggc atcgagacgg cggggacggc
     1021 gctgggcgca tcctcggcgg ggctcaccgt tggcgccgtt gcgagctgct tcgcgctgcg
     1081 ctggctgtcg ctgcgcttcg gctggaacct gccgcggttc cggcggccgg gcgcgcacga
     1141 gggcctggac tcgatggtgg agtgagccaa agccccggcc gcgcacgccc attcgccgcg
     1201 gacgagaatc ggccgcttcc gcaaccccgc ccgacgacga cgatgaccgc cttcgacagc
     1261 ctgagcgact tccccatcac cgccaagtgg cccgcgcggc atcccgaccg gctgcagctg
     1321 tactcgctgc cgacgcccaa cggcgtcaag gtctcgatcg cgctggagga gaccggcctg
     1381 ccctacgagc cgcacctggt gcgcttcgac gccaacgagc agatgtcgcc ggagttcctg
     1441 tcgctgaacc ccaacaacaa gatcccggcg atcatcgacc cgaacggtcc gggcggccgg
     1501 ccgctgccgc tgttcgagtc gggcgccatc ctcgtctacc tcgccggcaa gacgggccag
     1561 ctgatgcccg ccgacgacgc cggccgctac gaggtcctgc agtggctgat gttccagatg
     1621 ggcggcatcg ggccgatgtt cgggcaggtc ggtttcttcc acaagttcgc cggcagggac
     1681 ttcgaggaca agcgcccgcg cgaccgctac gtcgccgagg cgcgtcgcct gctggcggtg
     1741 ctggacggcc acctcgacgg ccgcgagtgg atcgtgggcg accgctactc gatcgcggac
     1801 atcgccagct ttccctggat caacaacctg gtcggcttct acaacgcccg cgagctcgtc
     1861 ggcttcgacg acttcgccaa cgtgaagcgc gtgctcgcgg ccttcctcga gcggcccgcg
     1921 gtgcagcgcg ggctcaagat cccggcgcgc gactgacctt ggggcgcggc gccgccggtc
     1981 gaggccgggc gccgcggctg tcccttcagc gccggcgcgc gacgagcgcc aggccggcca
     2041 gccccgccgt catcagcgcg aggctgcccg gctcggggac gccggggggg ccgttcatgt
     2101 ggctgcagcg gccgcccgcg gcgccgccgc cgatgtcgag ctcgccgcag ccgtagcgcg
     2161 aatccaccga ggcaacgtac cagccgaagg tgtcgccggc gcggacgacc acggacgcga
     2221 agcccgagct gccctgcccg gggtcgccgt cgatcgacag ctgggtctcg acgccgttca
     2281 ggatccagcc ggccgggtcg tagacggcgc cgccgctgtc gaaggtctcg tacgtccagc
     2341 tgaaggcgac gacggcgtcg gtcgcgaagg tctggacgta gaacgcggtg ttcggcaggc
     2401 cgtcggcgct ggccgggtcg ccgttgttcg agccgaagat cgagaacgac ggctcggtgc
     2461 cgacaagcgt gccgtcgccg ccgccgtcgt tcgagaacac gtaggccgcg cgggcgccac
     2521 ccgccaggcc gaaggcgacg agggcggccg ccgccagcgc cgcggcgcgg gaaagcttgg
     2581 gcatgggaat cctcctggaa cggcgtccgg cgcctgcgct cccgctggcc ggacggtgcg
     2641 gcggatcgtg gaagaacccc gcgccggcct caatgccggg cagagcgcca acctaggggg
     2701 agccgccggg cggcgagccg ggagcgactt cggcgcgccc gggggtcacg cgcgggcgca
     2761 gccgggactc acccgagtgc cagcgccatc tgcaggtcgt ttcgtatgtc ggtgccgatg
     2821 cgcatcgcgt cgctctcccg gccgaagacg acgaagccga gcgattcgta gagcgcgatc
     2881 gccgcgacgt tggcggcgtc gacttccagc gtcaccttgg ccaggcccgc ggtcgagcgc
     2941 gcgagatcca gcgccgcgac catcagctgc cgcgccaggc ccagccggcg cgcggagggc
     3001 gcgacgtaca tgccccagac gatgcccttg tgcgccatct tggcgaagcg cgcccgggcg
     3061 agacccacca cgcccacgag ctggccggcc tcgaacgcgc cgagcacgcc gggatcgcgc
     3121 tcgtcgacca ggcggcgggc gacctcgccc agcggcaggt ccttctcctc ctcgtggctg
     3181 gaggcgaagg cctccggcgt atcgagcagg gcggcaagcc gcaaggcctg gtaggcggcg
     3241 gcgtcttcgg tgcaaagtcg gcggatggtc atggctttga tttacgcttc gcgcgagtct
     3301 atcgggcagg cggcgggccc gtcacgcggc cgcccgggat gccccccttt tcacgaaagg
     3361 atccgcgatg aagacccgcg cagccgtggc ctggaaggcc ggtgcccccc tcaccatcga
     3421 gaccgtcgac ctggagggcc cgcgcgcggg cgaggtgctg gtcgaggtca aggccaccgg
     3481 catctgccac accgaccagt tcacgctgtc gggggcggat cccgaaggcc tgttccccgc
     3541 catcctcggc cacgaggggg cgggcgtggt gctggaggtc ggtgccggcg tgacgagcct
     3601 gaggcccggc gaccacgtca tcccgctcta cacgcccgag tgccggcagt gcaagttctg
     3661 cctctcgcgc aagaccaacc tgtgccagca gatccgcgcg acgcagggca aggggctgat
     3721 gcccgacggc acgagccgct tctcgctcga cggccgcccc atcctgcact acatgggcac
     3781 gtcgaccttc gccaaccaca tcgtggcgcc cgagatcgcg ctcgcgaaga tccgccccga
     3841 cgcgcccttc gacaaggtct gctacatcgg ctgcggcgtg accaccggcg tcggggcggt
     3901 gctgttcacg gccaaggtcg aggccggcgc caacgtggtc gtgttcgggc tcggcggcat
     3961 cgggctgaac gtgatccagg gcgcccgcat ggtcggcgcc gacaggatcg tcggcatcga
     4021 catcaacccc gcgcgcgagg cgatggcccg ccggttcggc atgacgcact tcctcaaccc
     4081 gagggagcac gccaacatcg tcgacgccat cgtccagctg accgacggcg gcgccgacta
     4141 cagcttcgag tgcatcggca acgtggcgac gatgcgccag gcgctggagt gctgccacaa
     4201 gggctggggc aagagcgtga tcatcggcgt cgcgggcgcg gggcaggaga tctcgacacg
     4261 gccgttccag ctcgtcaccg ggcgcgagtg gaaaggctcg gccttcggcg gcgcgcgcgg
     4321 ccgcaccgac gtgccgcgca tcgtcgactg gtacatggac ggcaagatct cgatcgaccc
     4381 gctcatcacg cacacgctgg agctcgagga catcaaccac ggcttcgagc tgatggagcg
     4441 cggcgagtcg atccgctcgg tggtggtgta ctgatggccg gcggagagat cgagatcctg
     4501 tccgagcacg cctgcttcgg cggcgtgcag ggcttctacc ggcacgcgtc ggcgacgatc
     4561 ggcctgccga tgcgcttcgg cgtcttccgg ccgccgcagg cgctggacgg caggccggtg
     4621 cccaccctct tctggctcgc cggcctgacc tgcaacgagg agaccttcgc gatcaaggcg
     4681 ggcgcccagc gcgtggctgc ggagctcggg ctgatgatcg tcacgcccga caccagtccg
     4741 cgcgagaccg gcgtgccgga cgcggacaag gactgggact tcggcgtcgg tgccggcttc
     4801 tacctcgacg cgacgcaggc gccctgggcg atgcactggc gcatggagag ctggatcacg
     4861 cgcgagctgc ccgaggtgct cgcggcgcgc tttccgtggc ggcgcgagcg caccgggatc
     4921 ttcggccact cgatgggcgg ccacggggcg ctgacgctgg cgctgcggca tccgggccga
     4981 tacgcgagcg tctccgcctt cgccccgatc gccgcgccct cgcaggcgcc ctggggcatc
     5041 aaggcgttca ccggctacct cggcgccgat cgcgcggcct gggcggcgca cgacgccgag
     5101 gcgctggtgg cgtccggcgc acgggcgccg tcgctgctcg tcgaccaggg ccaggccgac
     5161 aagttcctcg ccgaccagct gcacgtcgac cgcttcgagt ccacctgccg cgccgccggc
     5221 cagccgctcg tcttgcgccg gcatgccggc tacgaccacg gctacttctt catccagagc
     5281 ttcatggagg atcacctgcg ctttcacgcg gacagcctgg cgcgggtgtg agcggcaggc
     5341 acgcgggcac gcccgcgcac gaaccgggaa tccggcaagc ccttcatgcg gcgccggccg
     5401 agccgtcggc gtcgcggcgt ggaggccgac ctggtcttca ggccccggac cgccgtgccc
     5461 gccgccgctg cacgccgccc accaccgcgc cgcacagcat cagtccgcac gcgccggcga
     5521 gcagcagcgc gccccgctcg tcgaactgcg cgccgttgtc cagggcggac tggcgggcgc
     5581 gcaccaggtc ctcgcgccgc tggagttcat ccgcgctcgg gacgaaggcc actcgctggc
     5641 ccgcggcgtc gacataggtg ccgggcctgc ccgacaacgc gtagttgatc cgcgcaccct
     5701 ccaggctctt gcgggtgcgc tcctcgggcg gaatggcttg cttctcccag ccgcccggcg
     5761 gctcctcggc cttcatcgcg gcctcggtgc ccagcatcac gctgaccgcg gtcgcgaagc
     5821 gcaggctcat cacgccctct cccaccagcg gcacgaggcc cgctaccagc agcaggaccc
     5881 tcaggcgccc gaagccgccc tgcaacagcc gccggctgcc ctcgtagacg gcgatggccg
     5941 cggcagcgag taccagcgcc tccaggcaaa gttcgagcga gcccatccag tcagacatct
     6001 cgtcacggca atcgaagtcg aagttcgcga ttgtcgtgcg gaccggacgg gcggcggcga
     6061 ggcatcgatg gaagccgttg cggctccggc gaacggcacg ccaacggcga cgaatcgccc
     6121 gggcagcggc tcaatgtccg gccacgaccg tgacgatctt caacgcctgc gcctgcgccc
     6181 agcgcagcgt cgcgggacgc gcgagcgaca tggccgcggc acggacgacg cgcacgctgt
     6241 ccgcgaccat cgcgcccatc gcgcccatcg cgcccaggcg agccgccggg aacgcgttcg
     6301 aaatcgaggc cgcggacggg ctcgcctggc ttgccgagat ctgcatgtcg ctctcctgtc
     6361 gctcggcgcg cttccacagg ccacgcgccg atcactgtat ggagatacag tctatgcgat
     6421 cgatggacgc gtgcaagcga aagaccgtgc ggtcgtgtcg gtttgcacag tgcacggcgg
     6481 cgcggctcag gcgggacgca gcgggatgca gagctcgcac tcgaagcggc cggtatcggc
     6541 ttcgtagcgg gcgtcggggc ggtagtactc gtagatcggg cgatcgtcca tgccgtagcc
     6601 gctggcgggc agccagtcgc gcagcagttc ggtgtaggcg tgggcgatgt cgctcgagtc
     6661 gccgaagaac ggcgccacgg cgtagcgtcc gccaggcagg gtgtcgacgc ccgcgggcgc
     6721 ggcgggcatg acgccgtccg ggatcgggca gcagacgtcg tagcggcact tgtcgggcgg
     6781 ggtcaccgac gggtcgtcgc ggccgacgcc gtacagcggg ccgtcggcga gaccctgggc
     6841 ctgcagccag ggcaccgcga cgcgcagcca gaactcggcc accggcttgc cgtacggccc
     6901 gatgtggcgc aggtaggcca cgcgcgccgc gggcagggtc tcgatcgtca cttccatgaa
     6961 agcctccggg atggggatgc cgccatcgtc gctcgccacc ggaccgcccg cctgatcgtc
     7021 cttgcggtcc ctccgatccg cctcgcgccg aacgagccgg cggcgctgct cggcaagctc
     7081 ctgctgccaa cgctgcggcg tcgcccggcg ccatgcgctg ggcgtgcagc cgaaacggtg
     7141 cctgaaggcg cgcgcgaacg cctcgcccga gccgaagccc accgccagcg ccacctccag
     7201 gacgctgcgc cgcgcgttgc cggcgaggat cagcgccgcg cggtcgagcc gccggcggcg
     7261 caggtagtcg cccagcgtct cgcccatcca cgccgagaag acgcggtgga aatggaacgg
     7321 cgagaagtgc gcgaccgcgg ccagcgtcac gaggtcgagc tcgacatcca ggcgcgcgtc
     7381 gatgtggtcg agcacgcggt tcatgcgctg cgcgtaggcg gcggcgtggc gccgggcggt
     7441 ctgcggatcg aggctcatcg cgcgtgctcc agccaggcga ggacgacggg tttccggagc
     7501 ccgcgcacgg tacacgatgt cagtcgtccc gctcggcccc gagctccgcc gcgaggttcg
     7561 cgtcgacgcg c
//
LOCUS       DASVLN010000671         5158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_519768, whole genome shotgun sequence.
ACCESSION   DASVLN010000671 DASVLN010000000
VERSION     DASVLN010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519768"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..578)
                     /locus_tag="VF453_15885"
     CDS             complement(114..578)
                     /locus_tag="VF453_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7689195.1"
                     /translation="MSHEPLMNYFAAAPAATNALVAFAKTAGAKLDRRLAELVNLRVS
                     QINGCAFCMDMHSHDLLGQGVDPRALATLAGWREARRFFSPRECAALAWAESVNAIPN
                     RVPSEEEFAALREHFKDSEIAELTLAICAIRSFNALNASFRTPVPEKPYTGA"
     gene            complement(639..1502)
                     /locus_tag="VF453_15890"
     CDS             complement(639..1502)
                     /locus_tag="VF453_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX7689196.1"
                     /translation="MDTSTSELFEGLRPRLFGVAYRMLGMRADAEDVLQDAWLRWREA
                     DHAGLQSAEAWLVTVVTRLAIDRLRAQRLERERYVGDWLPEPIAVDAADTPEGIVERA
                     SQLSLAFMHLLERLGPEERAAFLLRQAFDYDYGEIAPMLGKSEAAVRQMVHRAGERLR
                     SDRPRFEVPGEQHRRLLERFARAASSGERSAIRALFADEVEVVGDGGGKASAFSGLRT
                     GDRITNLFWAHALRFGPRLEYRLATINGEPGVLRYIDGRLDTAQTFDVEGDRIVAMYA
                     VRNPDKLARIG"
     gene            1707..2096
                     /locus_tag="VF453_15895"
     CDS             1707..2096
                     /locus_tag="VF453_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689197.1"
                     /translation="MRIDADQQARMKRTHAAGTLRAVIAHCHREEGDPIDQETAEALV
                     DVGARLAHEYGMRGDYACGILAYLTWQFGEDFERVIPPIRAILLDGSIEDKPNALLDA
                     AARFWDIWSAGAGDVGAADGHLGGRRR"
     gene            2093..3079
                     /locus_tag="VF453_15900"
     CDS             2093..3079
                     /locus_tag="VF453_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689198.1"
                     /translation="MMTTLRGLRFVSRLRKASTASPSTLASAARLFSTSSGRGGGMTS
                     SQRARDTHKAATMIASSSACTHCQARFVEAIQSQVPPGRDLDDDEQDLIARKLAACDG
                     DPERESALAKGIVAVLTKDALLAGASRDGLLAYESKPLSELMAQAADKDTSAPQVERA
                     LAAISQPSGVPGGTDPNPGSTPVPGLPKEDYAKLRKKTPSQAIRDRVNQGVTLPMDDP
                     ALPSLEITSTLHADHIVPMKEITQMPGFAELTEEHQLQVLNNEANFVGLSEIANTSKG
                     AKSFEEWTEYKKLGIKVDEQFRQTMIKKADQMRPRLQGQIAQLLAAQRAASE"
     gene            3096..3815
                     /locus_tag="VF453_15905"
     CDS             3096..3815
                     /locus_tag="VF453_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX7689199.1"
                     /translation="MLTRQEISACMGRFGRTGELLLDLADLARADDIPAAWIDFLHGS
                     DEERIARLVAYWDPVASRLPAVTAFVRDKVHPPAILREPSGDVSLLYPHRTAQGGLHL
                     YQGYAPLGGLGGDLPLWKALPDDLKRFYRDIHNGWTYMADNAMGPLPVGDLAMLDGVM
                     DMSEAEIDRLSPSPRSVLRVFHNGTGSYICLDLGKSRGDGWNAGILWEAEEPEKLQRV
                     DFWAYLDGWMGIFFENAETRA"
     gene            3908..4255
                     /locus_tag="VF453_15910"
     CDS             3908..4255
                     /locus_tag="VF453_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689200.1"
                     /translation="MRMTRLLLAAALGGAATSLLVRAARRASPSVGVWDPHGDAADEN
                     EDGDAIVNRDAAIVADDALAPGASAGERIAEAAGAGSVLRRDDTHADLLASNSREGAF
                     ATSSGLSDFTRGA"
     gene            complement(4259..5092)
                     /locus_tag="VF453_15915"
     CDS             complement(4259..5092)
                     /locus_tag="VF453_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7689201.1"
                     /translation="MPFVFVVLWSTGFIGARLGLPHIEPLTFLTLRYAIVLVLMTGVA
                     LAVRAPWPRGMRAWLHIGGSGLLIQATYLGGVFIAIGRGLPAGVTSIVVGLQPLLTAV
                     AAHAFLGESVGRRQWIGLLLGFAGVGMVVFGKAGTGFTATQLVPAVVALFAITAGTIW
                     QKRFCPAFDGRTGMVAQFLPTLVATGIAAWCTETMRIDWAPGLFAALAWLVLVLSIGA
                     ITLLNWLIRHGDVSNIAALFYLVPPCTALFAWLFFHDTLTALALAGMALAAWGVWLAR
                     R"
BASE COUNT          828 a         1864 c         1825 g          641 t
ORIGIN      
        1 ggccgcgccg tcacctgcac gagccgggcg gccgacttca cgttcgcgct gcaggtcgcg
       61 ccgcggcgcg tcgtcgacgc ccggcccgcg gacgccgcac cccggcccga agctcaggcg
      121 cccgtgtagg gcttctcggg caccggcgtc cggaacgacg cgttcagcgc attgaacgag
      181 cggatggcgc agatcgccag ggtcagctcg gcgatctccg agtccttgaa gtgctcgcgc
      241 agcgcggcga actcctcctc gctgggcacg cggttcggga tggcgttgac ggactcggcc
      301 caggccagcg ccgcgcactc gcgcgggctg aagaagcggc gcgcctcgcg ccagccggcc
      361 agcgtggcga gcgcacgcgg gtcgacgccc tggccgagca ggtcgtgcga gtgcatgtcc
      421 atgcagaacg cgcaaccgtt gatctgcgag acgcgcaggt tgacgagctc ggccaggcgg
      481 cggtcgagct tcgcgccggc cgtcttggcg aaggcgacga gcgcgttcgt ggccgcgggg
      541 gcggcggcga agtagttcat gaggggctcg tgggacatcg tggactcctg ggtggttgaa
      601 tccattagac gccgccagcg gccgtcctgt gacagccgtc agccaatccg ggcgagcttg
      661 tcgggattgc gcacggcgta catcgcgacg atgcgatcgc cttccacgtc gaaggtctgc
      721 gccgtgtcca gccgcccgtc gatgtagcgc agcacgccgg gctcgccgtt gatcgtggcg
      781 agccggtatt cgaggcgcgg cccgaagcgc agcgcgtgcg cccagaacaa gttggtgatg
      841 cggtcgccgg tgcgcagccc cgagaacgcg gacgccttgc cgccgccgtc gccgacgacc
      901 tcgacctcgt cggcgaacag cgcccggatc gccgagcgct cgccgctcga ggcggcgcgc
      961 gcgaagcgct ccagcagccg ccggtgctgc tcgcccggca cctcgaagcg aggacgatcc
     1021 gagcgcagcc gttcgccggc gcggtgcacc atctggcgca cggccgcctc gctcttgccg
     1081 agcatgggcg cgatctcgcc gtagtcgtag tcgaacgcct ggcgcagcag gaacgcggcg
     1141 cgctcctccg ggcccagccg ctcgagcagg tgcatgaacg cgagcgagag ctggctcgcg
     1201 cgctcgacga tgccctcggg cgtgtcggcc gcgtcgaccg cgatcggctc gggcagccag
     1261 tcgccgacgt agcgctcgcg ctccaggcgc tgtgcgcgca gccggtcgat ggccagccgc
     1321 gtcacgacgg tgaccagcca cgcctccgcc gactgcaggc ctgcgtggtc ggcctcgcgc
     1381 cagcgcagcc aggcgtcctg caggacgtcc tcggcgtccg cgcgcatgcc cagcatgcgg
     1441 taggcgacgc cgaacaggcg gggccgcaag ccctcgaaga gttcgctggt ggaagtgtcc
     1501 atggctcgta gacgcgggag acggccggct tgtgacaccg gctgcgcgcg agtgtcgcgc
     1561 atcggcgccg ggccgtcgac cgccgccgcc caggcgacgt ccgggaggcc ccggggcacc
     1621 atcgacggcg gttcgccttt tcgcaaccgc ggcccctgcc tttgagatac cctacgcggt
     1681 atcaaaagac agggagagga cgcgacatgc ggatcgacgc cgaccaacag gcgcggatga
     1741 agcggacgca cgcggcaggc acgctccgcg ccgtcatcgc ccactgccac cgcgaagagg
     1801 gcgaccccat cgaccaggaa accgcggaag cgctggtcga cgtcggcgcc cggctggcgc
     1861 acgaatacgg catgcgcggc gactacgcct gcggcatcct ggcctacctc acctggcagt
     1921 tcggggagga cttcgagcgc gtgatcccgc ccatccgcgc catcctgctc gacggttcga
     1981 tcgaggacaa gccgaacgcc ctgctcgacg cggcggcgcg gttctgggac atctggagcg
     2041 ccggcgcggg cgacgtcggc gccgcggacg gccacctcgg cggacgccgg cgatgatgac
     2101 caccctgcgg gggctgcgct tcgtctcgcg gctcaggaag gccagcaccg ccagtccctc
     2161 gaccctggct tccgcggcca ggctgttcag cacgagttcc gggcgcggcg gcggcatgac
     2221 gtcgagccag cgtgcgagag acacccacaa ggcggccacg atgatcgcgt cgtcctcggc
     2281 ctgcacccac tgccaggcca ggttcgtcga agccatccag tcgcaggtgc cgcccgggcg
     2341 cgacctcgat gacgacgaac aggacctcat cgcccgcaag ctcgccgcct gcgacggcga
     2401 tcccgagcgc gaatcggcgc tcgccaaggg catcgtcgcc gtgctcacga aggacgccct
     2461 cctggccggc gcgagccggg acggcttgct cgcgtacgag agcaagccgc tctcggagct
     2521 gatggcgcag gctgccgaca aggacacctc cgcgccccag gtcgagcggg cgctcgccgc
     2581 gatcagccag ccctccggcg tcccgggcgg cacggatccg aatccgggca gcacgcctgt
     2641 cccgggcctg ccgaaggaag actacgcgaa gctgcgcaag aagacgccgt cgcaggcgat
     2701 ccgggacagg gtcaaccagg gcgtcacgct gccgatggac gacccggcgc tgccaagcct
     2761 ggagatcacg tcgacgctgc acgccgacca catcgtgccg atgaaggaga tcacgcagat
     2821 gcccggcttc gcggaattga ccgaggaaca ccagctgcag gtcctgaaca acgaggccaa
     2881 cttcgtcggc ctcagcgaga tcgcgaacac ctcgaagggc gccaagtcct tcgaggagtg
     2941 gacggaatac aagaagctcg gcatcaaggt ggacgagcag ttccgccaga ctatgatcaa
     3001 gaaggcggac cagatgcggc cgcgattgca aggccagatc gcccagctcc tggctgcgca
     3061 gcgcgccgcc tccgaatgac cgaggactcc catcgatgct caccagacaa gagatttccg
     3121 catgcatggg caggttcggg cggaccggcg agctcttgct ggacctggcg gacctggcgc
     3181 gcgcggacga catccccgcc gcctggatcg acttcctcca cggctcggac gaggagcgga
     3241 tcgccaggct ggtcgcctac tgggatccgg tcgcgtcgcg attgcccgcg gtgacggcct
     3301 tcgtgcggga caaggtgcat ccgcccgcga tcctgcggga gccttccggc gacgtctcgc
     3361 tgctgtatcc ccacaggacc gcgcaaggcg gcctgcatct ctaccagggc tacgccccgc
     3421 tgggcggact cgggggcgac ctgccattgt ggaaggcgct tcccgacgac ctcaagcgct
     3481 tctaccggga catccacaat ggatggacct acatggccga caacgccatg ggcccgctgc
     3541 cggtcggcga cctggccatg ctggacggcg tcatggacat gagcgaggcg gagatcgacc
     3601 gcctgtcgcc ttcgccgcgg agcgtcctgc gcgtcttcca caacgggacg ggcagctaca
     3661 tctgcctgga cctgggcaag agccggggtg acggctggaa cgccgggatc ctctgggagg
     3721 cggaggagcc cgagaagctg cagcgggtcg acttctgggc ctacctcgac ggctggatgg
     3781 gcatcttctt cgagaacgcc gagacgcgag cctgaccgcg gagcacgcct gggcaggcgg
     3841 gcaggcacgc cttgcccgac ggggcaccgc gcttgctccg cggcgtcatc gaatcccgga
     3901 gacccccatg cgaatgaccc gcctgctcct cgccgccgcc ctcggcggtg ccgccacgtc
     3961 cctgctcgtg cgcgccgcgc gccgcgcctc gccgtccgtc ggcgtgtggg atccccacgg
     4021 cgacgccgcg gacgagaacg aggacggcga cgccatcgtc aaccgcgacg cggccatcgt
     4081 cgccgacgac gcgctcgccc ccggcgcgag cgccggcgag cggatcgccg aagcggccgg
     4141 cgccggctcg gtgctgcgcc gcgacgacac gcacgccgac ctgctcgcat cgaactcccg
     4201 ggagggggcg ttcgcgacct ccagcgggct gtccgacttc acgcgcggcg cctgatcctc
     4261 agcgccgtgc gagccagacg ccccacgccg cgagcgccat gcccgcgagc gcgagggcgg
     4321 tcagcgtgtc gtggaagaac agccacgcga acagcgccgt gcacggcggc acgaggtaga
     4381 acagcgcggc gatgttggag acgtcgccgt ggcggatcag ccagttcagc agcgtgatcg
     4441 cgccgatcga caggaccagc accagccagg cgagcgccgc gaacaggccg ggcgcccagt
     4501 cgatgcgcat cgtctccgtg caccaggcgg cgatgccggt ggccaccagg gtcggcagga
     4561 actgcgcgac catcccggtg cgaccgtcga aggccggaca gaagcgcttc tgccagatcg
     4621 tgcccgccgt gatggcgaac agggcgacca ccgccggcac gagctgggtt gccgtgaagc
     4681 ccgtgcccgc cttgccgaac accaccatcc cgacgccggc gaagccgagc agcagcccga
     4741 tccactgccg cctcccgacc gactcgccga ggaaggcgtg cgccgcgacc gccgtcagca
     4801 gcggctgcag gccgaccacg atgctcgtca cgcccgcggg caggccgcgc ccgatcgcga
     4861 tgaagacgcc gcccaggtag gtcgcctgga tcagcaagcc cgagccgccg atgtgcagcc
     4921 acgcccgcat cccgcgcggc cacggcgcgc gcacggcgag cgcgacgccg gtcatcagca
     4981 ccagcacgat cgcgtagcgc agcgtgagga aggtgagcgg ctcgatgtgc ggcagcccga
     5041 gccgcgcgcc gatgaagccg gtcgaccaca gcaccacgaa cacgaacggc agcgccgcgt
     5101 cgaggcgcgc ccgtggggcg gcggaagcgg gcgacgagcg cggcgtggcc ggcgccgg
//
LOCUS       DASVLN010000672         8191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_356651, whole genome shotgun sequence.
ACCESSION   DASVLN010000672 DASVLN010000000
VERSION     DASVLN010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356651"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            414..3254
                     /gene="secA"
                     /locus_tag="VF453_15920"
     CDS             414..3254
                     /gene="secA"
                     /locus_tag="VF453_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX7689202.1"
                     /translation="MLPKLLTSIFGSRNDRLLKQYRRIVDKIAALEGPTAALDEAALK
                     GKTAEFRARLAQGTPLDDLLPEAFAVVREAGKRALKMRHFDVQLIGAMSLHFGKIAEM
                     RTGEGKTLMATLAVYLNALEGKGVHLVTVNDYLAKRDAEWMGRLYNYLGMTVGVNVPG
                     LTREEKQAAYAADITYGTNNEFGFDYLRDNMVYETADRVQRGLNFAIVDEVDSILIDE
                     ARTPLIISGQAEDHTDLYVRINAVVPALKRQIGEEDPRTGEGVIEPGDFTVDEKAHQI
                     TLTEAGHERAEELLAQAGLLPEGASLYDAANITLMHHVYAALRAHHLYHRDQHYVVQN
                     GVDEETGRPVQEVVIVDEFTGRLMTGRRWSDGLHQAVEAKEGVKIQSENQTLASITFQ
                     NYFRMYAKLGGMTGTADTEAYEFQEIYGLETVVIPPNRPTQRKDELDLVYKTAREKYD
                     AVVQDIRDCVERGQPVLVGTTSIENSELLHNLLVKAGVPHQVLNAKQHEREALIVAQA
                     GRPGTVTIATNMAGRGTDIVLGGNVEKQIEFIEADESIPAEEKAARIQQLKDEWQALN
                     AQVKAAGGLRIVATERHESRRIDNQLRGRSGLQGDPGSSRFYLSLEDPLMRIFAGDRV
                     RAIMDRLKMPEGEAIEAGIVSRSLEGAQRKVEARNFDMRKQLLEYDDVANDQRKVIYQ
                     QRNEILEASSLVNQIANLREGTMTDIVRAYVPEESVEEQWDLPGLEKLLREEWQFDAP
                     IAKLVEDSDTLTDEDVVEAVVKAAAAHFQVKLDLVGEAQFTPFMRMVLLQHIDSHWRE
                     HLAALDYLRQGIHLRGYAQKNPKQEYKREAFELFAQLIDLVKTEVTRVLMTVRVQTQE
                     QAAAAAQAIERRAEQVDNVQYTHPNEDGSVSQETAAALAAQRVVSYGFSDDRPAGMPE
                     LPDGVPLQRVGRNDPCPCGSGKRFKECHGKLA"
     gene            3368..5095
                     /locus_tag="VF453_15925"
     CDS             3368..5095
                     /locus_tag="VF453_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7689203.1"
                     /translation="MAFEKNFFPRQVVEAGNRWDWALLPIVLAAIVLAALGGVGMTRP
                     YHLGDPIPLSLDPLQLPYYLLRTTLRMLLALMCSLLFSFAFATAAARWRAAEKVLVPL
                     LDILQSIPILGFLAITVTGFIRLFPGNLFGVECAAIFAIFTSQAWNMAFSLYQSMRTV
                     PSELQEAARVFQLSPWQRFWRLELPFAMPGLLWNMMMSMSGGWFFVVASEAISVSNQN
                     IKLPGVGSYIAMAIEARNLQAIGWAILGMLIGIVLYDQLLFRPLVAWADKFRFEESGQ
                     ESAPTSWLLTWSRRSERIAKLAARFAELIESSFTWFRQAYDGTSIRARPLPASPALAR
                     AWDALIAAGGLFAIYRLVTFVGDEVSFAEVGHVFLLGFLTMLRVVILIGLAALVWVPV
                     GVWIGMRPQWSGRLQAVAQFLAAFPANLMFPVFVVFIVHWHLNANVWLSPLMVFGTQW
                     YLLFNVIAGASSIPAELRFAAGNIGLSGWLRWKRFLLPAVFPSFVTGAITASGGSWNA
                     SIVSEYVTWGSTKLEAAGLGSYIAHSTADGDFPRIALGIGVMCVFVMGMNHFVWRRLY
                     RIAEERMHF"
     gene            5215..6552
                     /locus_tag="VF453_15930"
     CDS             5215..6552
                     /locus_tag="VF453_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7689204.1"
                     /translation="MAQSIIELHGVRKSFRSADGRQRPVLEGVDFTLREGEIVALLGQ
                     SGSGKSTLLRLMAGLIGVDAGTVQYRGQPLYGPARAISMVFQSFALFPWLTVQQNVEL
                     GLEARGIEREERAERAQKAIDMIGLAGFEGALPRELSGGMRQRVGIARALVTQPDVLL
                     MDEAFSALDVLTGERLRDDILELWAQGGMPTKAILVVSHNIEEAVMMADRVLVFASDP
                     GRIRFQLAINLPRPRVADSADVRSLIDEVYALMTAGASRAGRTTEEAGEPALRLHDRL
                     PAADVARMDGLLELLAGAPFHGEADLPKLAEESELTDDELLPVASAISLLGLARLQSG
                     DLHLTLAGRRYVDGDHATRQQLFGQQLLENVPLAAHIRHSLEQDPERALPEQPFLRLL
                     EQHLDAGEAERVLQTVIEWGRHGEVFEYDFHAGSIHLPDDQQDEQASRNSVIE"
     gene            6675..7919
                     /gene="argJ"
                     /locus_tag="VF453_15935"
     CDS             6675..7919
                     /gene="argJ"
                     /locus_tag="VF453_15935"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX7689205.1"
                     /translation="MPVNLAAPNPADLHPVPGVRLGVAMAGVRKAGRKDLVALVLDAG
                     SVVAGVFTRNRFCAAPVQVCRDHLAAGAADGADGIRALVINTGNANAGTGADGLARAR
                     RTCADAAGLLGLKPQQVLPFSTGVIMETLPVERIAAALPAALADLAPGRWAEAAAGIM
                     TTDTIPKAASTQANIGGRTVTVTGISKGAGMIRPNMATMLGFVATDAVVAPAVVQELV
                     RRAADASFNRITIDGDTSTNDSFVLVATQQAGHARIDSLDSEDGRALLAAVTKVSQQL
                     AQAIVRDGEGATKFIAVQVDGGKDEAECRLAAYAIAHSPLVKTAFFASDPNLGRILAA
                     VGYAGIADLDQARIDLFLDGVAVVKDGGRREDYREEDGARVMKQQEITVRIDLHRGDA
                     TTTVWTCDFSYDYVKINADYRS"
BASE COUNT         1236 a         2989 c         2802 g         1164 t
ORIGIN      
        1 acctcgcgcc gctgctgccg accggcccgc gctgacccgg ccgcgcgccc gtgcggggcg
       61 cgcgttttcc caggattccg gcgcccggcg gcggcacccc cgggtgctac acttgcccgt
      121 ttgacgcccc ttgccgggcg ggcccgatca cggcccgtgc gacgaggggc ggatcgaacg
      181 tgagccgaca ggcgcgaggg tttgtcctgc ccgcgcccgc gccttgccgt cgcgcgccag
      241 cgtccccaga ttgcgggggc ggcgcggcgt cccggggacg cgcgcctgaa acgcccacgt
      301 ccgacccacc cgcttgccac cccggcaggc ccgcggcttc cgagccggcc cagccgaccc
      361 cggccgccgc acgacgccac gctggcctgc tacacgccgg ccctgacaca cgcatgttgc
      421 ccaagctcct gacctcgatt ttcggcagcc gaaacgatcg cctgctgaag cagtaccggc
      481 gcatcgtcga caagatcgcc gccctcgaag gcccgaccgc cgcgctcgac gaggccgcgc
      541 tcaagggcaa gacggcggag ttccgcgccc gcctcgcgca gggcacgccg ctggacgacc
      601 tgctgcccga ggccttcgcc gtcgtgcgcg aggccggcaa gcgcgcgctc aagatgcgcc
      661 atttcgacgt ccagctgatc ggcgcgatgt cgctgcactt cggcaagatc gccgagatgc
      721 gcacgggcga gggcaagacg ctgatggcga cgctggccgt ctacctgaac gcgctcgagg
      781 gcaagggcgt gcacctggtg acggtcaacg actacctggc caagcgcgac gccgagtgga
      841 tgggccgcct ctacaactac ctcggcatga ccgtgggcgt gaacgtcccc ggcctgacgc
      901 gcgaggagaa gcaggccgcc tacgccgccg acatcaccta cggcacgaac aacgagttcg
      961 gcttcgacta cctgcgcgac aacatggtct acgagaccgc cgatcgcgtg cagcgcgggc
     1021 tgaacttcgc gatcgtcgac gaggtcgact cgatcctgat cgacgaggcc cgcacgccgc
     1081 tgatcatcag cggccaggcc gaggaccaca ccgacctgta cgtgcgcatc aacgcggtcg
     1141 tgccggcgct caagcgccag atcggcgagg aggacccgcg caccggcgag ggcgtcatcg
     1201 agccgggcga cttcacggtc gacgagaagg cgcaccagat cacgctgacc gaggccggcc
     1261 acgagcgcgc cgaggagctg ctggcccagg ccggcctgct gcccgagggc gcgagcctgt
     1321 acgacgcggc caacatcacg ctgatgcacc acgtgtacgc agccctgcgc gcgcaccacc
     1381 tgtaccaccg cgaccagcac tacgtcgtcc agaacggtgt cgacgaggag accggccgcc
     1441 cggtgcagga agtggtgatc gtcgacgagt tcaccggccg cctgatgacc ggccggcgct
     1501 ggtcggacgg cctgcaccag gcggtcgagg ccaaggaagg cgtgaagatc cagagcgaga
     1561 accagacgct cgcctcgatc acgttccaga actacttccg catgtacgcc aagctgggcg
     1621 gcatgaccgg cacggccgac accgaggcct acgagttcca ggagatctac ggcctcgaga
     1681 cggtcgtgat cccgccgaac cggccgacgc agcgcaagga cgagctcgac ctcgtctaca
     1741 agaccgcgcg cgagaagtac gacgccgtcg tccaggacat ccgcgactgc gtcgagcgcg
     1801 gccagccggt gctggtgggc actacctcga tcgagaactc cgagctcctg cacaacctcc
     1861 tggtcaaggc cggcgtgccg caccaggtgc tcaacgccaa gcagcacgag cgcgaggcgc
     1921 tgatcgtcgc gcaggcgggc cgccccggca ccgtgacgat cgccaccaac atggccggcc
     1981 gcggcacgga catcgtgctg ggcggcaacg tcgagaagca gatcgagttc atcgaggccg
     2041 acgagtccat cccggccgag gagaaggccg cgcgcatcca gcagctcaag gacgaatggc
     2101 aggcgctgaa cgcgcaggtc aaggccgcgg gcggcctgcg catcgtcgcc accgagcgcc
     2161 acgagtcgcg ccggatcgac aaccagctgc gcggccgctc cggtctccag ggcgacccgg
     2221 gctcctcgcg cttctacctg tcgctcgagg atccgctgat gcgcatcttc gcgggcgacc
     2281 gcgtgcgggc gatcatggac cgcctgaaga tgcccgaggg cgaggcgatc gaggccggca
     2341 tcgtctcgcg cagcctcgag ggcgcgcagc gcaaggtcga ggcgcgcaac ttcgacatgc
     2401 gcaagcagct gctggagtac gacgacgtcg ccaacgacca gcgcaaggtg atctaccagc
     2461 agcgcaacga gatcctcgag gcgagctcgc tggtcaacca gatcgccaac ctgcgcgagg
     2521 gcacgatgac cgacatcgtg cgcgcctacg tgcccgagga atcggtcgag gagcagtggg
     2581 acctgccggg cctcgagaag ctgctgcgcg aggaatggca gttcgacgcg ccgatcgcca
     2641 agctggtcga ggacagcgac accctcacgg acgaggacgt cgtcgaggcg gtcgtgaagg
     2701 cggcggccgc gcacttccag gtcaagctcg acctggtcgg cgaggcgcag ttcacgccct
     2761 tcatgcgcat ggtgctgctg cagcacatcg actcgcactg gcgcgaacac ctcgccgcgc
     2821 tggactacct gcgccagggc atccacctgc gcggctacgc ccagaagaac ccgaagcagg
     2881 agtacaagcg cgaggccttc gagctgttcg cgcagctcat cgacctcgtc aagacggagg
     2941 tcacgcgggt gctgatgacg gtgcgcgtgc agacgcagga gcaggccgcg gcggccgccc
     3001 aggcgatcga gcgccgcgcc gagcaggtcg acaacgtgca gtacacgcat cccaacgagg
     3061 acggcagcgt gagccaggag accgccgcgg cgctggccgc gcagcgtgtc gtgtcctacg
     3121 gcttcagcga cgaccgcccc gcgggcatgc ccgagctgcc cgacggcgtg ccgctgcagc
     3181 gcgtcggccg caacgacccg tgcccctgcg gcagcggcaa gcgcttcaag gagtgccacg
     3241 gcaagctggc gtgacggctt gatgacgcgc gcgtcgctgg cgcgccgaac caacgaagcc
     3301 gcatcggcgc cgccgcgatg cggctttcct ctttagaagc aaggttgagc acggacaatc
     3361 cagcgccatg gccttcgaga agaacttctt cccccgccag gtcgtcgagg ccggcaaccg
     3421 ctgggactgg gcgctgctgc ccatcgtcct ggcggcgatc gtgctggcgg cgctcggcgg
     3481 cgtgggcatg acgcgcccgt accacctcgg cgatccgatc ccgctgtcgc tcgacccctt
     3541 gcagctgccg tactacctgc tgcgcacgac gctgcgcatg ctgctggcgc tgatgtgctc
     3601 gctgctgttc agcttcgcct tcgcgaccgc ggcggcgcgc tggcgcgcgg ccgagaaggt
     3661 gctggtgccg ctgctcgaca tcctgcagtc gatccccatc ctcggcttcc tcgcgatcac
     3721 ggtgaccggc ttcatccgcc tgttccccgg caacctgttc ggcgtcgagt gcgcggcgat
     3781 cttcgcgatc ttcacctcgc aggcctggaa catggcgttc agcctgtacc agtcgatgcg
     3841 caccgtgccg agcgagctgc aggaggccgc gcgcgtgttc cagctgtcgc cctggcagcg
     3901 cttctggcgc ctggagctgc cgttcgcgat gcccggcctg ctgtggaaca tgatgatgtc
     3961 gatgtcgggc ggctggttct tcgtcgtcgc gtcggaggcg atctcggtct ccaaccagaa
     4021 catcaagctg cccggcgtcg gctcctacat cgcgatggcg atcgaggcgc gcaacctgca
     4081 ggcgatcggc tgggccatcc tcggcatgct gatcggcatc gtgctgtacg accagctgct
     4141 gttccggccg ctggtggcct gggccgacaa gttccgcttc gaggagagcg gccaggagtc
     4201 ggcgccgacc tcgtggctgc tgacctggtc gcgccgcagc gagcgcatcg ccaagctcgc
     4261 cgcgcgcttc gccgagctga tcgagagctc gttcacctgg ttccgccagg cctacgacgg
     4321 cacgtccatc cgcgcccggc cgctgcccgc gtcgcccgcg ctcgcgcgcg cgtgggacgc
     4381 gctgatcgcc gccggcggcc tgttcgcgat ctaccggctc gtgaccttcg tcggcgacga
     4441 ggtgagcttc gccgaggtcg gccacgtgtt cctgctcggc ttcctcacga tgctgcgcgt
     4501 cgtgatcctg atcggcctcg cggccctggt ctgggtaccg gtcggcgtat ggatcggcat
     4561 gcggccgcag tggtcgggcc gtctgcaggc ggtggcgcag ttcctcgcgg cgttcccggc
     4621 caacctgatg ttcccggtct tcgtggtgtt catcgtgcac tggcacctga acgccaacgt
     4681 ctggctgtcg ccgctgatgg tgttcggcac gcagtggtac ctgctgttca acgtcatcgc
     4741 gggcgcgtcc tcgatcccgg cggagctgcg cttcgccgcc ggcaacatcg gcctgtcggg
     4801 gtggctgcgc tggaagcgct tcctgctgcc cgccgtcttc ccgagcttcg tcaccggcgc
     4861 catcacggcc agcggcggct cgtggaacgc gagcatcgtc tccgaatacg tcacctgggg
     4921 cagcaccaag ctggaggcgg ccggcctcgg cagctacatc gcgcactcga ccgccgacgg
     4981 cgacttcccg cgcatcgcgc tgggcatcgg cgtgatgtgc gtgttcgtga tgggcatgaa
     5041 ccacttcgtg tggcgccgcc tctaccgcat cgccgaagag cgcatgcatt tttagtcatg
     5101 acgacccccg ctcactccgt tcgccgcccc ccgagggggc gctcggcttg ctccggagcg
     5161 gccgagcgcg gcgccgagcc ttccgccaac cgatagattc aacggacttt cgacatggcc
     5221 caatccatca tcgaactgca cggcgtgcgc aagtccttcc gctcggcgga cgggcgccag
     5281 cggcccgtgc tcgagggcgt cgacttcacg ctgcgcgagg gcgagatcgt cgcgctgctc
     5341 gggcagtcgg gctcgggcaa gagcacgctg ctgcggctca tggccggcct gatcggcgtc
     5401 gacgccggca ccgtgcagta tcgcggccag ccgctgtacg gcccggcgcg cgcgatcagc
     5461 atggtcttcc agtccttcgc gctgttcccg tggctgaccg tgcagcagaa cgtcgagctc
     5521 gggctggagg cgcgcggcat cgagcgcgag gagcgcgccg agcgcgccca gaaggcgatc
     5581 gacatgatcg gcctcgccgg cttcgagggc gcgctgccgc gcgagctctc gggcggcatg
     5641 cgccagcgcg tgggcatcgc gcgcgccctc gtcacgcagc ccgacgtgct gctgatggac
     5701 gaggccttct cggcgctgga cgtgctcacc ggcgagcgcc tgcgcgacga catcctcgag
     5761 ctctgggccc agggcggcat gccgaccaag gcgatcctcg tggtctcgca caacatcgag
     5821 gaggcggtga tgatggccga ccgcgtgctg gtcttcgcga gcgacccggg gcgcatccgc
     5881 ttccagctcg cgatcaacct gccgcgcccg cgcgtggccg actcggccga cgtgcgttcg
     5941 ctgatcgacg aggtctacgc gctcatgacc gcgggcgcga gccgtgccgg ccgcacgacc
     6001 gaggaggccg gcgagcccgc gctgcgcctg cacgaccgcc tgcccgccgc cgacgtggcg
     6061 cgcatggacg gcctgctgga gctgcttgcc ggcgcgccct tccacggcga ggcggacctg
     6121 cccaagctgg ccgaggaatc ggagctcacc gacgacgagc tgctgcccgt ggccagcgcc
     6181 atctcgctgc tgggcctcgc gcgcctgcag agcggcgacc tgcacctgac gctcgccggc
     6241 cgccgctacg tcgacggcga ccacgcgacg cgccagcagc tgttcgggca gcaactgctc
     6301 gagaacgtgc cgctcgcggc gcacatccgc cacagcctcg agcaagatcc cgagcgcgcg
     6361 ctgcccgagc agccgttcct gcgcctgctc gagcagcacc tggacgccgg cgaggccgag
     6421 cgcgtcctgc agacggtcat cgaatgggga cgccacggcg aggtcttcga gtacgacttc
     6481 cacgccggca gcatccacct gcccgacgac cagcaggacg agcaggcgtc gaggaactcg
     6541 gtcatcgagt aagcgccggc gtgaccgggc cgcgtggctc gcccggcggc ggacgcagcc
     6601 gctgaccgcc gccgtgcatg cgggcgtcac tgcgcgatcc tagaatcgcg cttctgccaa
     6661 aggagcccct cgccatgccc gtcaatctcg ccgccccgaa tcccgccgac ctgcatcccg
     6721 tcccgggcgt gcgactgggc gtcgcgatgg ccggcgtgcg caaggccggc cgcaaggacc
     6781 tggtcgcgct ggtgctggac gcgggcagcg tggtcgcggg cgtgttcacg cgcaaccgct
     6841 tctgcgccgc gcccgtgcag gtctgccgcg atcacctggc cgctggcgcc gccgacggtg
     6901 cggacggcat ccgcgcgctc gtgatcaaca ccggcaacgc caacgccggc accggcgcgg
     6961 acggcctggc gcgggcgcgc cgcacctgcg cggacgccgc cggcctgctg ggcctgaagc
     7021 cgcagcaggt gctgccgttc tcgaccggcg tgatcatgga gacgctgccc gtcgagcgca
     7081 tcgccgccgc gctgccggcc gcgctcgccg acctcgcgcc cggtcgctgg gccgaggccg
     7141 cggccggcat catgaccacc gacaccatcc ccaaggccgc ctcgacgcag gcgaacatcg
     7201 gcggccgcac cgtcaccgtc acgggcatca gcaagggcgc cggcatgatc cgccccaaca
     7261 tggcgacgat gctgggcttc gtggccaccg acgcggtggt ggccccggcg gtcgtgcagg
     7321 agctcgtgcg gcgcgccgcg gacgcctcgt tcaaccgcat caccatcgac ggcgacacgt
     7381 cgaccaacga cagcttcgtg ctggtggcca cgcagcaggc cggccacgcg cgcatcgact
     7441 cgctcgactc cgaggacggc cgcgcgctgc tggcggcggt caccaaggtt tcgcagcagc
     7501 tcgcccaggc catcgtccgc gacggcgagg gcgcgaccaa gttcatcgcc gtgcaggtcg
     7561 acggcggcaa ggacgaggcc gagtgccgcc tcgcggccta cgcgatcgcg cactcgccgc
     7621 tcgtcaagac ggcgttcttc gcgagcgatc cgaacctcgg ccgcatcctg gccgcggtcg
     7681 gctacgcggg catcgccgac ctcgaccagg cgcgcatcga cctgttcctc gacggcgtcg
     7741 cggtcgtcaa ggacggcggc cgccgcgagg actaccgcga ggaggacggc gcgcgcgtga
     7801 tgaagcagca ggagatcacg gtgcgcatcg acctgcaccg cggggacgcc acgacgaccg
     7861 tgtggacctg cgacttctcg tacgactacg tgaagatcaa cgcggactat cggagctgag
     7921 gatcccctcg tcttgacgcg cgatgccggc cgatgtcgcc ttttcgcccg tcgaccggca
     7981 gggcctcgct tgccggcggc aggcggccgc tgctaggatc gacgctcgct tcctccaaga
     8041 cgcatcgccc atgaaccgcc gcatccacgc ccaccgacga cgcctcgcgc gcggcgtggc
     8101 ctcgcgcggc ttcgtcgtgg cggtcgcgct cgtgggtgcc gtggccctgc agctcgcggg
     8161 gccgcgcctg cagtgccgcc tggcgtcgat c
//
LOCUS       DASVLN010000673         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_466033, whole genome shotgun sequence.
ACCESSION   DASVLN010000673 DASVLN010000000
VERSION     DASVLN010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466033"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VF453_15940"
     CDS             <1..267
                     /locus_tag="VF453_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689206.1"
                     /translation="GAAPATPAAPPSAPAFVFVHGKRRPAPPPPHEPVGFDNLSVLTW
                     SDARPTMVVTFHEHAGRSARETVLRQYWELDRDEWKIAAEGTVR"
     gene            415..921
                     /locus_tag="VF453_15945"
     CDS             415..921
                     /locus_tag="VF453_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689207.1"
                     /translation="MNKLSPATLLTVGATLITIGATFGIGRSHAATRPASASASAASA
                     RYQQDRANCLAGKTYEDRKTCLREAAAAFQAARSHDLASGDAEMYAANERKRCDPLTG
                     DDKKACLMRVAGQGTTSGSVAGGGELHTLVQEIPGQVPEPESLPPTAAGTAQPLAPQP
                     APSQPSGN"
     gene            complement(997..1476)
                     /locus_tag="VF453_15950"
     CDS             complement(997..1476)
                     /locus_tag="VF453_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7689208.1"
                     /translation="MIPKNTICLWFDGTALDAATFYAETFPDSRVGAVHRAPGDYPAG
                     QQGDVLTVQFTVLGIPCLGLNGGPQFRHTEAFSFQVATDDQAETDRYWNAIVGNGGQE
                     SACGWCKDRFGLSWQITPRALTDAFTDPDRAAAKRAFDAMMTMHKIDIATIEKARRG"
     gene            complement(1633..3453)
                     /gene="lpdA"
                     /locus_tag="VF453_15955"
     CDS             complement(1633..3453)
                     /gene="lpdA"
                     /locus_tag="VF453_15955"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7689209.1"
                     /translation="MAVIEVQVPDIGDFKDVGVIEVLVKPGDAVKQEQSLITVESDKA
                     SMEIPSSAAGVVKELRVKVGDKVNQGSVVLTLDAEGAAAPAPAPAAPIGTSAPATPAR
                     TESQAPAAAPVNAPAGPAPVPATYAGGADLECDMLVLGGGPGGYSAAFRAADLGMKVV
                     LVERYATLGGVCLNVGCIPSKALLHVAAVMDEVGHFAALGIDYGAPKVDLDKLRAHKG
                     KVVGKLTGGLAAMAKMRKVTVVRGVGAFVDPHHLKVDETSGEGQQVTGSSKTVKFKQA
                     IIAAGSQAVRLPFFPQDPRIVDSTGALELRGTPKKMLIVGGGIIGLEMGTVYSTLGAR
                     LDVVEMLDGLMQGADRDLVKVWQKMNAKRFDSIFLKTKTVGAEATSDGIKVKFEGEGG
                     VREEMYDLVLQAVGRVPNGKKIGADKAGVVVGDRGFIPVDVQMRTNVPHIFAIGDIVG
                     QPMLAHKAVHEGHVAAEVAAGDAHARFDARVIPSVAYTDPEVAWVGLTEDQAKAQGIA
                     VKKGLFPWSASGRAIANGRDEGQTKLLFDAGTHRIVGGGIVGMNAGDMIGEVALAIEM
                     GADAVDIGRTIHPHPTLGESIGLAAEVAEGACTDLPPARK"
     gene            complement(3494..>4224)
                     /locus_tag="VF453_15960"
     CDS             complement(3494..>4224)
                     /locus_tag="VF453_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX7689210.1"
                     /translation="GLLPWPKVDFEKFGVVERKDLSRIKKISGANLHRNWVVIPHVTN
                     HDDADITELEAFRVTLNKEYEKAGVKVTMLAFLIKACVAALKKFPEFNASLDGEQIVL
                     KKYFHIGFAADTPNGLVVPVIRDADQKGVVQIAREMGELAAKARDGKLGPADMTGGCF
                     SISSLGGIGGTYFTPIINAPEVAILGVCKSFMKPVWDGKAFVPRLILPLSLSWDHRVV
                     DGAAAARFNAYLGQILADFRRVML"
BASE COUNT          599 a         1546 c         1425 g          654 t
ORIGIN      
        1 ggcgccgccc ccgcgacgcc cgccgcgccg ccgtccgcgc cggcgttcgt gttcgtgcac
       61 ggcaagcgcc gcccggcgcc gccgccgccg cacgagccgg tcggcttcga caacctgagc
      121 gtcctcacct ggtcggacgc caggccgacc atggtcgtca ccttccacga gcacgccggc
      181 cgcagcgcac gcgagaccgt gctccgccag tactgggagc tcgaccgcga cgagtggaag
      241 atcgccgccg agggcacggt gcgctgatcg cgcggccgcg ccgcgcggct cgatgcccgc
      301 cgtatcgcgc ctgcgcgcga atccatccgg atctcccgcg cgaccgggcg aggtggcatg
      361 cccctgggta cgtcgattgc tgcatcgatg caatcgctta ccggagcaac caccatgaac
      421 aagctatcgc ctgcgacgct gttgacggtc ggcgcgaccc tgatcacgat cggagcgacc
      481 ttcggcatcg gcaggagcca tgccgccacc cgacccgcct ccgcctccgc ctcggccgcc
      541 tcggcccgct accaacagga ccgcgccaac tgcctggccg gcaagaccta cgaggaccgc
      601 aagacctgcc tgcgcgaggc cgcggccgcg ttccaggccg cccgcagcca cgacctggcg
      661 agcggcgacg ccgagatgta tgccgccaac gagcgcaagc gctgcgatcc gctgaccggc
      721 gacgacaaga aggcctgcct gatgcgcgtc gcgggccagg gcacgaccag cggcagcgtc
      781 gccggcgggg gcgagctgca tacgctggtg caggagatcc ccggccaggt gcctgagccc
      841 gagagcctgc cgcccacggc cgccggcacg gcacagccac tcgcgccgca gccggcgccg
      901 tcccagccgt ccggcaactg aaggatcgcc tgccgtggat cgattccggg tggtggggcg
      961 ccgcacggcc ggccgccgag gaccggcggc tgccgctcag ccgcgccgtg ccttctcgat
     1021 cgtggcgatg tcgatcttgt gcatcgtcat catcgcgtcg aacgcgcgct tggcggcggc
     1081 gcgatccggg tcggtgaacg cgtcggtcag cgcgcgcggg gtgatctgcc aggacaggcc
     1141 aaagcgatcc ttgcaccagc cgcaggcgct ctcctggccg ccgttgccga cgatggcgtt
     1201 ccagtagcgg tcggtctcgg cctggtcgtc ggtcgccacc tggaacgaga acgcctcggt
     1261 gtggcggaac tgcggcccgc cgttgaggcc caggcaggga atccccagca ccgtgaactg
     1321 caccgtgagc acgtcgccct gctggccggc gggatagtcg cccggcgcgc ggtgcactgc
     1381 gccgacgcga ctgtcgggga aggtctcggc atagaaggtc gcggcgtcca gcgccgtgcc
     1441 gtcgaaccag aggcagatcg tgttcttcgg gatcaaggtg gtctcctgtc gtgtcgcgtc
     1501 cagcagggat ttgtgtacgc cgtccacaag ataccggctc aaatggacag tgtccacaaa
     1561 tttcgcaatt cccttccgga aacgaacgag ggcgcccgcg ggcgccctcc tcggccgggc
     1621 cgtcgggcag ccctacttgc gcgccggcgg caggtccgtg cacgcgcctt cggcgacctc
     1681 ggccgcgagg ccgatcgact cgcccagcgt cgggtgcggg tggatggtgc ggccgatgtc
     1741 gaccgcgtcc gcgcccatct cgatggccag cgcgacctcg ccgatcatgt cgcccgcgtt
     1801 catgccgacg atgccgccgc cgacgatgcg gtgcgtgccg gcgtcgaaca acagcttggt
     1861 ctggccctcg tcgcggccgt tggcgatcgc gcgacccgag gccgaccacg ggaacaggcc
     1921 cttcttgacc gcgatgccct gcgccttggc ctggtcctcc gtgaggccca cccacgcgac
     1981 ctcggggtcg gtgtaggcga cgctcgggat gacgcgcgca tcgaagcgcg catgggcgtc
     2041 gcccgccgcg acctcggcgg cgacgtggcc ctcgtgcacc gccttgtgcg cgagcatcgg
     2101 ctggccgacg atgtcgccga tcgcgaagat gtgcggcacg ttcgtgcgca tctgtacgtc
     2161 caccgggatg aagccgcggt cgccgacgac cacgcccgcc ttgtcggcgc cgatcttctt
     2221 gccgttgggc acgcgcccca ccgcctgcag cacgaggtcg tacatctcct cgcgcacgcc
     2281 gccctcgccc tcgaacttca ccttgatgcc gtccgaggtc gcctccgcgc ccacggtctt
     2341 cgtcttcagg aagatgctgt cgaagcgctt cgcgttcatc ttctgccaga ccttgaccag
     2401 gtcgcggtcg gcgccctgca tcaggccgtc cagcatctcg acgacatcca ggcgcgcgcc
     2461 cagcgtcgag tacacggtgc ccatctccag gccgatgatg ccgccgccga cgatcagcat
     2521 cttcttcggc gtgccgcgca gctccagcgc gccggtcgag tcgacgatgc gcgggtcctg
     2581 cgggaagaac ggcaggcgca ccgcctgcga gccggccgcg atgatggcct gcttgaactt
     2641 caccgtcttg ctcgagccgg tgacctgctg gccctcgccc gaggtctcgt cgaccttcag
     2701 gtgatgcgga tccacgaacg cgccgacgcc gcgcacgacg gtgaccttgc gcatcttggc
     2761 catcgccgcc aggccgcccg tgagcttgcc cacgaccttg cccttgtgcg cgcgcagctt
     2821 gtcgaggtcc accttcggcg cgccgtagtc gatgcccagc gcggcgaaat ggccgacctc
     2881 gtccatcacg gccgcgacgt gcagcagcgc cttcgacggg atgcagccga cgttcaggca
     2941 gacgccgccc agcgtggcgt agcgttcgac cagcacgacc ttcatgccga ggtcggcggc
     3001 gcggaacgcc gcggaatagc cacccgggcc gccgcccagc accagcatgt cgcactcgag
     3061 gtcggcgccg ccggcatacg tggcagggac cggcgccggg ccggccggcg cgttcaccgg
     3121 cgccgcggcg ggcgcctgcg actccgtgcg tgccggcgtg gccggcgcac tggtaccgat
     3181 gggcgccgcg ggagcgggcg ccggcgcggc cgcgccctcg gcgtccagcg tcagcaccac
     3241 gctgccctgg ttcaccttgt cgccgacctt gacgcgcagc tccttgacga cgcccgccgc
     3301 cgacgacggg atctccatcg acgccttgtc gctctcgacg gtgatgaggc tctgctcctg
     3361 cttgacggcg tcgcccggct tgaccagcac ctcgatgacg ccgacgtcct tgaagtcgcc
     3421 gatgtccggc acttgcactt cgatcacggc catgtgtgtc tcgcttggtg ttcgttgttc
     3481 gtgaccggcg cgatcacagc atcacgcgac ggaagtcggc gaggatctgg cccaggtacg
     3541 cgttgaagcg cgcggccgcg gcgccgtcga cgacgcggtg gtcccacgac agcgacagcg
     3601 gcaggatgag gcgcggcacg aaggccttgc cgtcccacac cggcttcatg aagctcttgc
     3661 agacgcccag gatcgcgact tccggcgcgt tgatgatcgg cgtgaagtag gtgccgccga
     3721 tgccgcccag cgagctgatc gagaagcagc cgccggtcat gtcggccggg cccagcttgc
     3781 cgtcgcgcgc cttggccgcg agctcgccca tctccctggc gatctgcacg acgcccttct
     3841 ggtcggcgtc gcggatgacg ggcacgacca ggccgttggg cgtgtcggcg gcgaagccga
     3901 tgtggaagta cttcttcagg acgatctgct cgccgtccag cgacgcgttg aactccggga
     3961 acttcttcag cgccgcgacg caggccttga tcaggaacgc gagcatcgtg accttgacgc
     4021 cggccttctc gtactccttg ttcagcgtga cgcggaaggc ctcgagctcg gtgatgtcgg
     4081 cgtcgtcgtg gttggtgacg tgcgggatca ccacccagtt gcgatgcagg ttcgcgcccg
     4141 agatcttctt gatgcgcgag aggtccttgc gctccacgac gccgaacttc tcgaagtcta
     4201 ccttgggcca cggcagcagg cccg
//
LOCUS       DASVLN010000674         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_519794, whole genome shotgun sequence.
ACCESSION   DASVLN010000674 DASVLN010000000
VERSION     DASVLN010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519794"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /gene="sctJ"
                     /locus_tag="VF453_15965"
     CDS             complement(<1..625)
                     /gene="sctJ"
                     /locus_tag="VF453_15965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HEX7689211.1"
                     /translation="MNALFRSVRRLALPLVAAALLAACGQQDLYAQLDEEQANEMVAV
                     LRNAGLTADKEAREGQTFAVVAPQSDFARAITVLRANGYPRDSFDSMGRVFKKDGFVS
                     TPVEEHARLTHALSQEIANTLSHIDGVVVARVQLALPEKDPLADKPQPAAAAVFIKHR
                     PGVDLSGSVGQIKALVVDSVEGLPYDNVTVALFPAQDWPEAAAALPAP"
     gene            complement(693..1112)
                     /locus_tag="VF453_15970"
     CDS             complement(693..1112)
                     /locus_tag="VF453_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689212.1"
                     /translation="MTIDLSAAALHAALPPAATGAGHGLQAGYGVSLTDLSGFQEALR
                     AAAARGGQGELQTHADNRVSPAVQALFQPLEHINGEATSLQADAAAALRAGPEMSPGD
                     MVQLTVRCQEFMFHCELTSNMANRASDGVQQLFRQQS"
     gene            complement(1208..1522)
                     /locus_tag="VF453_15975"
     CDS             complement(1208..1522)
                     /locus_tag="VF453_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689213.1"
                     /translation="MTTTTLDSSLSALAAAPVATSSPSDSGNGSGDWFQAMAQAWSNT
                     LDNEANKISSMSDSIGSGQDDPQAITMLTTESLRMGYLSDSSHTSLNSVGQALDTMAR
                     KG"
     gene            complement(1550..>1826)
                     /locus_tag="VF453_15980"
     CDS             complement(1550..>1826)
                     /locus_tag="VF453_15980"
                     /inference="COORDINATES: protein motif:HMM:NF028003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscD/YscD/HrpQ family type III secretion system
                     periplasmic domain-containing protein"
                     /protein_id="HEX7689214.1"
                     /translation="AGQTARRDVAGLRELVVRNDPPAPAPQDRPVADDPGKRVAAIVP
                     GDAPYVATADGTRYFIGALLPTGHRVEAIEPHRVVLSRDGRQSELDF"
BASE COUNT          226 a          609 c          720 g          271 t
ORIGIN      
        1 tcggcgccgg cagggcggcc gcggcctcgg gccagtcctg ggccgggaac agcgcgaccg
       61 tcacgttgtc gtagggcagc ccctcgacgc tgtcgacgac gagcgccttg atctggccga
      121 cgctgcccga gaggtcgacc cccggccggt gcttgatgaa gaccgcggcc gcggcgggct
      181 gcggcttgtc ggcgagcgga tccttctccg gcagcgcgag ctgcacccgc gcgacgacga
      241 cgccgtcgat gtgcgacagc gtgttggcga tctcctgcga cagcgcgtgc gtgaggcgcg
      301 cgtgctcctc gaccggcgtc gagacgaagc cgtccttctt gaacacgcgg cccatggagt
      361 cgaagctgtc gcgcggatag ccgttggcgc gcagcacggt gatggcgcgc gcgaagtcgg
      421 actgcggggc gacgacggcg aaggtctggc cctcgcgcgc ctccttgtcc gcggtcaggc
      481 cggcgttgcg caggacggcc accatctcgt tggcctgctc ctcgtcgagc tgcgcgtaga
      541 gatcctgctg gccgcaggcg gcgagcagcg ccgccgccac gagcggaagc gcgagtcggc
      601 gcacgctgcg aaacagcgcg ttcatcgggg aacctccggc ctcgcggcct gcggtgcgag
      661 cgccttcggt cggccgggcg gcattcatgg cgtcagctct gctggcggaa cagctgctgc
      721 acgccgtcgg acgcgcggtt ggccatgttg gaggtcagct cgcaatggaa catgaactcc
      781 tggcagcgca ccgtcagctg caccatgtcg ccgggcgaca tctcgggccc ggcgcgcagc
      841 gcggcggcgg cgtcggcctg caggctggtc gcctcgccgt tgatgtgctc cagcggctgg
      901 aagagcgcct gcaccgcggg gctgacgcgg ttgtcggcgt gcgtctgcag ctcgccctgg
      961 ccaccgcggg cggcggcggc gcgcagcgct tcctggaagc cggacaggtc ggtcagcgaa
     1021 acgccgtagc cggcctgcag gccgtggccg gcgccggtgg ctgccggcgg caacgccgcg
     1081 tggagcgcgg cggcggagag gtcgatcgtc atccggggtc ctccatggcg ataccgggcg
     1141 ggtatcaccg gaaatcggga tcgcgggcgg cgcggcggcc gcccgcgggg cgtcggcgcg
     1201 cgcgccgtca gcccttgcgc gccatcgtgt cgagcgcctg gccgaccgag ttcagcgagg
     1261 tgtgcgagct gtccgacagg tagcccatgc gcagcgactc ggtggtcagc atcgtgatgg
     1321 cctggggatc gtcctggccg ctgccgatcg agtcggacat cgacgagatc ttgttcgcct
     1381 cgttgtcgag cgtgttgctc caggcctggg ccatcgcctg gaaccagtcg ccggagccgt
     1441 tgcccgagtc cgagggcgag ctcgtggcga ccggcgcggc ggccagcgcg gacagggagg
     1501 aatcgagggt ggtggtggtc atggggtctc tccttggttg gatggggggt cagaagtcga
     1561 gctcgctctg gcgcccgtcc cgcgacagga cgacgcggtg cggttcgatg gcctcgacac
     1621 ggtgtccggt gggcaggagc gcaccgatga agtagcgcgt gccgtcggca gtcgccacgt
     1681 agggagcgtc gcccggcacg atggccgcga cgcgcttgcc cgggtcgtcc gccacgggcc
     1741 tgtcctgggg tgccggggcg ggcgggtcgt tgcgcacgac gagctcgcgc aggccggcga
     1801 cgtcgcggcg cgcggtctgg ccggcg
//
LOCUS       DASVLN010000675         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_144447, whole genome shotgun sequence.
ACCESSION   DASVLN010000675 DASVLN010000000
VERSION     DASVLN010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144447"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1109)
                     /locus_tag="VF453_15985"
     CDS             complement(93..1109)
                     /locus_tag="VF453_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7689215.1"
                     /translation="MTQPSRRQILGAAAAFAAAGIASTARAQAPITIKFSHVVAPDTP
                     KGKGAMRFKELAEQRTNGKVKVEVYPNSQLYKDKEEMEALQLGSVQMLAPSLSKFGPL
                     GVKEFEVFDLPFLFKDTEAFRAVTEGALGKELFAKLEPKGIRGMAYWDNGFHIMSANR
                     PLHHVADFKGLKMRIQSSKVLDAQMRALGAIPQVMAFSELYQALQSGVVDGTEGTPSN
                     FWTQKIYEVQKHMTLSNHGHLAYAAIVNKKFWDGLPNDIRTTLDGCMHDATVYANAIA
                     ATENSQDLEKIKASGKTTVYQPTAAEILEWKKALMPVHKEMEGRVGKDAVAAAYKAAG
                     FVPV"
     gene            1323..>2805
                     /locus_tag="VF453_15990"
     CDS             1323..>2805
                     /locus_tag="VF453_15990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7689216.1"
                     /translation="MAIEPPSELPLPPSPLPAAPVGRAWARHLARRGAWWWVPLVLSG
                     LFTVSVAAWLQHSDSVDLESRQRQLITDSLSLESQIGDRVAEEQRELDALARSFDPSL
                     PATALAQQEEVTDGLARLWQSISWVDADERLRAVVPERADLVVGDRPGISAHLVAALH
                     DAAGNPAGHLVVRYSPGLLLRQTVPWWLAHEYNVRLVDGFGQVTADPLHAGAATFSPA
                     ESHRHSLEPAMADTYLELSLKESPVPWFLRVPVVLMGVFLVLIGAVTALLRWQMNELL
                     RTQAALRTEAAWRQAMEDSLIVGLRARDNEGRLVYVNRAFCDLVGYGPEALIGRATPM
                     PYWPPDAIADSLSRHLRNMAGGAPREGYEARWVRADGRLIDVMIFEAPLVDASGRRIG
                     LMGSILDITERKRLEERERHRTDAVAHQARLTMLGEVASALAHQLNQPLTSITGYAAG
                     VKRLLERAGQPDARLVDAMTRLGDQAAEAGRIVKRIREFLTRRA"
BASE COUNT          353 a          979 c         1039 g          434 t
ORIGIN      
        1 cgcggcggca tcggacgccc agtggacgac ggccccgcgg gccgcgtcga tcgtgacggc
       61 cccggcgccg cgcgccgggc cgcacggcgg gatcagaccg gcacgaagcc cgcggccttg
      121 taggcggcgg ccacggcgtc cttgccgacg cggccctcca tctccttgtg caccggcatc
      181 agcgccttct tccattcgag gatctcggcg gccgtcggct ggtagaccgt ggtcttgccg
      241 ctcgccttga tcttctcgag gtcctgcgag ttctcggtcg cggcgatcgc gttggcgtag
      301 acggtggcgt cgtgcatgca gccgtccagc gtcgtgcgga tgtcgttggg caggccgtcc
      361 cagaacttct tgttgacgat ggcggcgtag gcgaggtggc cgtggttgga cagcgtcatg
      421 tgcttctgca cctcgtagat cttctgcgtc cagaagttcg acggcgtgcc ctcggtgccg
      481 tcgacgacgc ccgactgcag cgcctggtac agctcgctga aggccatgac ctgcgggatc
      541 gcgccgaggg cgcgcatctg ggcgtccagc accttggacg actggatgcg catcttcagg
      601 cccttgaagt cggccacgtg gtgcagaggc cggttcgcgc tcatgatgtg gaagccgttg
      661 tcccagtacg ccatgccgcg gatgcccttg ggctcgagct tggcgaacag ctccttgccc
      721 agcgcgccct cggtgaccgc gcggaaggct tcggtgtcct tgaacaggaa gggcaggtcg
      781 aagacctcga attccttgac gcccagcggg ccgaacttgg acagcgacgg ggcgagcatc
      841 tgcacggagc cgagctgcag ggcctccatc tcctccttgt ccttgtacaa ctggctgttc
      901 ggatagacct cgaccttgac cttgccgttg gtgcgctgct cggcgagctc cttgaagcgc
      961 atggcgccct tgcccttggg cgtgtcgggc gccacgacgt ggctgaactt gatcgtgatc
     1021 ggggcctgcg cgcgcgcggt ggacgcgatg ccggcggcgg cgaaggcggc ggcggcgccc
     1081 aggatctggc ggcgggacgg ttgggtcatg gtgcgttgtc tcctgagatg ttcgtcgggg
     1141 tggggatccg gtcatgccgg ggcggcgccc gctcgggtcg gggcctgcgc cggggggcgc
     1201 gcacggacgc gccgaagtgg gacggattgt cggcagcgcg gccgcgtcgc ggaaattgtg
     1261 gactcccaca tccgccggac gccggccggg ccgcggtccg ggagagcccc tagacttcgc
     1321 cgatggccat cgaaccgccc tccgagctgc cgctgccgcc ctcgcccctg ccggcggcgc
     1381 cggtcggccg cgcctgggcg cgccatctcg cgcggcgcgg cgcgtggtgg tgggtgccgc
     1441 tcgtgctgtc ggggctgttc accgtgtcgg tggcggcgtg gctgcagcac tccgacagcg
     1501 tcgacctcga gtcgcgccag cgccagctga tcaccgactc gctctcgctc gaaagccaga
     1561 tcggcgaccg cgtggccgag gagcagcgcg agctcgacgc cctcgcgcgc agcttcgacc
     1621 cgagcttgcc ggccacggcg ctcgcgcagc aggaggaggt gaccgacggc ctcgcgcgcc
     1681 tgtggcagag catcagctgg gtcgacgccg acgagcgcct gcgggccgtg gtgccggagc
     1741 gcgccgacct cgtcgtcggc gaccgcccgg gcatcagcgc gcacctggtc gcggcgctgc
     1801 acgacgcggc gggcaatccg gcggggcacc tggtcgtgcg ctactcgccc gggctgctgc
     1861 tgcggcagac ggtgccgtgg tggctcgcgc acgagtacaa cgtgcggctc gtcgacggct
     1921 tcggccaggt cacggccgat ccgctgcatg ccggggcggc gacgttctcg cccgcggagt
     1981 cgcaccgcca cagcctcgag ccggcgatgg ccgacaccta cctcgagctg tcgctgaagg
     2041 agtcgccggt gccgtggttc ctgcgcgtgc cggtggtgct gatgggcgtg ttcctcgtgc
     2101 tcatcggcgc ggtgaccgcc ttgctgcgct ggcagatgaa cgagctgctg cgcacgcagg
     2161 ccgcgctgcg cacggaggcg gcgtggcgcc aggcgatgga ggactcgctg atcgtcggcc
     2221 tgcgcgcgcg cgacaacgag gggcggctgg tatacgtcaa ccgcgcgttc tgcgacctcg
     2281 tcggctacgg gcccgaggca ctgatcggcc gcgccacgcc gatgccctac tggccgcccg
     2341 acgcgatcgc cgacagcctc tcgcgccacc tgcgcaacat ggcgggcggc gcgccgcgcg
     2401 aaggctacga ggcgcgctgg gtgcgcgcgg acgggcgcct catcgacgtc atgatcttcg
     2461 aggcgccgct ggtcgacgcg agcggccggc gcatcggcct gatgggctcg atcctggaca
     2521 tcaccgagcg caagcgcctc gaggagcgcg aacgccaccg cacggacgcc gtcgcgcacc
     2581 aggcgcggct gacgatgctg ggcgaggtcg cctccgcgct cgcgcaccag ctgaaccagc
     2641 cgctgacctc gatcaccggc tacgcggccg gcgtcaagcg cctgctcgaa cgcgccgggc
     2701 agccggacgc gcggctcgtc gacgcgatga cgcgcctggg cgaccaggcc gcggaggcgg
     2761 gccgcatcgt caagcgcatc cgcgagttcc tcacgcgccg cgcgc
//
LOCUS       DASVLN010000676         6675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_304581, whole genome shotgun sequence.
ACCESSION   DASVLN010000676 DASVLN010000000
VERSION     DASVLN010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304581"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /gene="gcvT"
                     /locus_tag="VF453_15995"
     CDS             complement(<1..770)
                     /gene="gcvT"
                     /locus_tag="VF453_15995"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX7689217.1"
                     /translation="MSADTSAPLLQTPLHALHLELGARMVPFAGYAMPVQYPTGILAE
                     HRQCRHEAALFDVSHMGQLLLQGEDAAAALETLVPVDVVDLGLNRQRYGFFTNAQGGI
                     LDDLMITRREDGLMVIVNAACKEADLAHLRAHIGERCRIVTMFDRALLALQGPMAVHA
                     IARLAPGIEKLTFMTGGFFDVAGIPCYVTRSGYTGEDGFEISVAAADAEKLARTLLAQ
                     PEVKPVGLGARDSLRLEAGLCLYGHDITEATTPVEAALT"
     regulatory      complement(818..947)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(956..1049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1103..1585)
                     /locus_tag="VF453_16000"
     CDS             complement(1103..1585)
                     /locus_tag="VF453_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689218.1"
                     /translation="MNKLLAALIASLGVSLAMAQAPAASSADVSKAEAKAEKNDAKAE
                     QKEAKTDAKADKKDAKADQKASKKKAEAHADAEKSKSKAQAKVDKADAGDKAKEEAKA
                     EKTDAEANAKEAKKDASADKSAEKTHASTKADRAKAKAKADETKAEDKAKVDKAAAGK
                     "
     gene            complement(1829..3193)
                     /locus_tag="VF453_16005"
     CDS             complement(1829..3193)
                     /locus_tag="VF453_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7689219.1"
                     /translation="MHPDGLPLPQRYASMLAIAVGITMAVLDSSVANIALPSIARQLG
                     VDPAGAVWVINAYQLAIVVLLLPLAALGERIGYRRVYLAGLALFTAGSLACALSGSLA
                     ALILSRIGQGLGAAGIMAVNGALVRYTYPQASLGRGVGLNALVVSAAGAIGPTVASGV
                     LAVGPWEWLFAINVPIGVANVVFAARVLPWSTRSDRALDWGSAALSALAFGLFFIGVD
                     MLTHDHGGVRTAAAELAGAAAAGAALFLRERHEARPLIPIDLLRIPIFGLSTATSICS
                     FAAYMLAFLALPFWFQSALHRDQVQTGLLMTPWPVGVGLVAPLAGRLADRIPAAILGS
                     AGLLLLASGLGLLAALPAEAATPDILWRMGLCGVGFGFFQAPNNRTLLGSAPRERAGA
                     AGGMLATARLTGMTSGATIAALVFRVAARDAEHVCLVIAVALALGAAALSASRLRARR
                     VAPA"
     gene            3332..3805
                     /locus_tag="VF453_16010"
     CDS             3332..3805
                     /locus_tag="VF453_16010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7689220.1"
                     /translation="MISDHPLRLAARVDATAIAALSRDGIEHGLPWRWRAPRVRQAID
                     DPATNVVVAGPPGELRGFGIMSYGDDDAHLLLLAVRDSWRRRGVGTAMLQWLEAAACV
                     AGARRVRLEARRDNEAARSFYNEHGYHERDLAPRMYGGRLDGVHLEKWLRPREGG"
     gene            complement(3903..4127)
                     /locus_tag="VF453_16015"
     CDS             complement(3903..4127)
                     /locus_tag="VF453_16015"
                     /inference="COORDINATES: protein motif:HMM:NF044532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689221.1"
                     /translation="MNISGSTLMMSIQAVHAEAERIVAAVGGQLDELLPDDQELLMAF
                     DKAAEELKQLYEVARKQEPGLTPYDQLVKT"
     gene            complement(4124..4600)
                     /locus_tag="VF453_16020"
     CDS             complement(4124..4600)
                     /locus_tag="VF453_16020"
                     /inference="COORDINATES: protein motif:HMM:NF037873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689222.1"
                     /translation="MTTTSARLYRSIRREDFPDGPIVGDAPAPAVLHPSFEDKPYTQN
                     TPEGSVQRVRPADTHPFPGKDGQMMVAPFKGTSLFDRENVFPSKFWWSFVIPEGTEVP
                     ASIVVRFTDYNKRFQANHYQIEPRAGIMRLDAYKGALDDLARCALVKYLAESKGQK"
     gene            complement(4787..4883)
                     /gene="ffs"
                     /locus_tag="VF453_16025"
     ncRNA           complement(4787..4883)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF453_16025"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5001..6128)
                     /locus_tag="VF453_16030"
     CDS             complement(5001..6128)
                     /locus_tag="VF453_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX7689223.1"
                     /translation="MSSHSSSSHPDTLSYEQAGVNYDLIDPLKVAAQRAAAATAGALA
                     GHGFSEVAASRGESAYVVDVGPFYLASIVECLGSKALVADEMAELTGRSFYAGIAQDT
                     IAMSINDLITVGATPLVVQAYWAAGGSDWFGDKLRAKALVDGWKAACDTCGVAWGGGE
                     TPALAGIVEGGRIDLASSCTGIVSPKSRLSVGDELQAGDAIVLLASSGIHANGLSLAR
                     KLVERLPHGYMTPVEPGLSYGEALLAPTTLYSPVTEGLAKAGLTVHYSANVTGHGWRK
                     LLRHPKPFTYRITQVPPVPPVLKFIQQHAKQDDREAYSTLNMGAGFALFMPAGQAEQA
                     VKIAQDLGIGAWIAGRVEDGPKQLLIEPIGVRFGDDDLQLR"
     gene            6195..>6675
                     /gene="dnaX"
                     /locus_tag="VF453_16035"
     CDS             6195..>6675
                     /gene="dnaX"
                     /locus_tag="VF453_16035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX7689224.1"
                     /translation="MSYVVLARKYRPRRFEQMVGQDHVVRALTHALEQGRLHHAYLFT
                     GTRGIGKTTVSRILAKSLNCTGPDGQGGITAHPCGQCTACTEIDADRYIDYVELDAAS
                     NRSIDEIRDLIERAAYKPSVGRYKVFMIDEAHQLTKDAFNALLKTLEEPPEYLKFVLA
                     "
BASE COUNT          967 a         2276 c         2416 g         1016 t
ORIGIN      
        1 gtgagcgcgg cctcgacggg ggtcgtggcc tcggtgatgt cgtggccgta gaggcacagg
       61 ccggcttcca ggcgcagcga gtcgcgcgcg cccaggccga cgggcttgac ctcgggctgc
      121 gcgagcagcg tgcgggcgag cttctcggcg tcggcggcgg ccaccgagat ctcgaagccg
      181 tcctcgccgg tgtagcccga gcgcgtgacg tagcacggga tgccggcgac gtcgaagaag
      241 ccgccggtca tgaacgtgag cttctcgatg ccgggcgcga ggcgcgcgat cgcgtgcacg
      301 gccatcgggc cctgcagcgc gagcagcgcg cggtcgaaca tcgtgacgat ccggcagcgt
      361 tcgccgatgt gcgcgcgcag gtgcgcgagg tcggcctcct tgcaggccgc gttgacgatc
      421 accatcaggc cgtcctcgcg gcgcgtgatc atcaggtcgt cgaggatgcc gccctgggcg
      481 ttggtgaaga agccgtagcg ctgcctgttc agcccgaggt cgacgacgtc gacgggcacc
      541 agggtctcga gcgcggccgc ggcgtcttcg ccctgcagca ggagctggcc catgtgcgag
      601 acgtcgaaga gcgcggcctc gtggcggcac tgccggtgct cggccaggat gccggtgggg
      661 tactgcaccg gcatggcgta gccggcgaag ggcaccatgc gggcgccgag ttcgaggtgc
      721 agggcgtgca gcggggtctg cagcagcggg gcggaggtgt cagcggacat ggtcgttcct
      781 tcgagggcca atcgatccgc ccatgatggc ggcttgaatt gccctcgctg tccgctttac
      841 ctgagagatt ggcggtgccg ggattgccgg cggccgcttg ccccttcggt ggacgggctc
      901 gcatgccgtc tccggcgaac cacccgtctc tctccagtga ggaacgccct ggcgggcgcc
      961 ttcgtcagtc cttttgcctg agcgttcggg gatggcagtc ccctgcgcct tcggcggccc
     1021 gatgaagggc tctctcctga caagcccgca gtctagcatc gtcgcgcggg acgcggccac
     1081 cggcccgtcc cgcgcgcggc gatcacttgc ccgcggcggc cttgtcgacc ttggccttgt
     1141 cctcggcctt ggtctcgtcg gccttggcct tggccttcgc gcgatcggcc ttggtcgagg
     1201 cgtgggtctt ctcggcgctc ttgtcggccg atgcgtcctt cttggcctcc ttggcgttgg
     1261 cctccgcgtc ggtcttctcg gccttggcct cttccttggc cttgtcgccg gcgtcggcct
     1321 tgtcgacctt ggcctgcgcc ttcgacttgc tcttctcggc atcggcgtgc gcctcggcct
     1381 tcttcttcga cgccttctgg tcggccttgg cgtccttctt gtcggccttg gcgtccgtct
     1441 tggcttcctt ctgttccgcc ttggcgtcgt tcttctcggc cttcgcctcc gccttcgaga
     1501 cgtccgcgct cgaggcggcc ggcgcctggg ccatggccag cgagacgccg agggaagcga
     1561 tgagggcagc aagcagcttg ttcatgtgaa aggttcctgg ttggtttctc gggagggtcg
     1621 accgaactgt caggcccgtg cgtgtacaac gcggggcccg ggacggttcg ttgacgcgat
     1681 acgtagaagc cgctgtccgg gctcgacagc gcggctgcgc atggcatttc gcgtgcccgc
     1741 ctgcccggcg gggcgcgtcc ggacataccc caggctgtat gcgcgcgccg acgcggcggc
     1801 acccctgcgc gattccgcgc ggacaggttc aggcaggtgc gacgcgccgg gctcgcagcc
     1861 gcgaggcgct cagggcggca gccccgagcg cgagcgcgac ggcgatcacg aggcacacgt
     1921 gctcggcgtc ccgcgcggcg acgcgaaaga cgagcgccgc gatcgtggcg ccgctggtca
     1981 tgcccgtcag ccgcgccgtg gccagcatcc cgccggccgc gccggcgcgc tcgcgcggcg
     2041 cgctgcccag cagggtgcgg ttgttggggg cctggaagaa gccgaagccc acgccgcaca
     2101 ggcccatgcg ccagaggatg tcgggcgtcg ccgcctcggc cggcagcgcg gcgagcaacc
     2161 cgagcccgct tgccaggagc agcagccccg cgctgccgag gatcgcggcg gggatgcggt
     2221 cggccaggcg gcccgccagc ggcgcgacga ggccgacccc gaccggccag ggcgtcatca
     2281 gcaggccggt ctgcacctgg tcgcggtgca gggccgactg gaaccagaac ggcagcgcga
     2341 ggaaggccag catgtaggcc gcgaacgagc agatcgaggt cgccgtcgac agcccgaaga
     2401 tcgggatgcg cagcaggtcg atcgggatca gcggcctggc ctcgtggcgc tcgcgcagga
     2461 acagcgcggc gcccgcggcc gcggcgccgg caagctcggc cgccgcggtg cggacgccgc
     2521 cgtggtcgtg cgtgagcatg tccacgccga tgaagaacag gccgaacgcg agcgcgctga
     2581 gcgccgcgct gccccagtcg agcgcgcggt ccgaccgcgt cgaccacggc agcacgcgcg
     2641 cggcgaagac gacattggcg acgccgatcg ggacgttgat cgcgaacagc cactcccacg
     2701 gcccgacggc cagcaccccc gaggccaccg tcggtccgat ggcccccgcg gccgacacga
     2761 cgagcgcgtt gagtcccacg ccccggccca gcgaggcctg ggggtaggtg tagcgcacca
     2821 gcgcgccgtt gacggccatg atgcccgccg cacccaggcc ctggccgatg cgcgagagga
     2881 tcaacgcggc cagggatccc gacagcgcgc aggcgagcga gccggccgtg aacagcgcga
     2941 ggcccgcgag gtagacgcgc cggtagccga tccgctcgcc gagcgcggcc agcggcagca
     3001 gcagcacgac gatcgcgagc tggtacgcgt tgatcaccca taccgccccg gccgggtcga
     3061 cgcccagctg gcgcgcgatc gagggcagcg cgatgttggc caccgacgag tcgagcaccg
     3121 ccatcgtgat gcccacggcg atggcgagca tggacgcgta gcgctgcggc agcgggaggc
     3181 cgtcgggatg gatggtgcgg atcaggatgg gctccgggtc gggcaaggcg gcgaggacag
     3241 gcgctgcgcg ggtcgcacgc gggcggcgcc ggaatgcgcg ggcgacggac cacccgtcgc
     3301 ggcatcatcg gggagcaacc cgcgcgccaa gatgatctcc gaccaccccc tccgccttgc
     3361 cgcccgtgtc gacgccaccg cgatcgcggc gctgtcgcgc gacgggatcg agcacggcct
     3421 gccgtggcgc tggcgcgcgc cgcgcgtgcg gcaggcgatc gacgatcccg ccaccaacgt
     3481 cgtcgtcgcc ggcccgcccg gcgagctgcg cggcttcggg atcatgtcct acggcgacga
     3541 cgacgcccac ctgctgctgt tggccgtgcg cgactcgtgg cgtcggcgcg gcgtcggtac
     3601 ggcgatgctg cagtggctcg aggccgccgc atgcgtcgcc ggcgcacgcc gcgtccgcct
     3661 cgaggcgcgg cgggacaacg aggccgcgcg cagcttctac aacgaacacg gctaccacga
     3721 gcgcgacctc gccccgcgca tgtacggcgg gcggctggac ggcgtgcatc tggagaagtg
     3781 gctgcggccg cgcgagggcg gatgagtccc gagctgcgcg cgacgccgga aacgaaaaga
     3841 ccgccaggcg ggcggtcctt cgaggggtgc cagagtgtct gccggcgaga ttcagaagag
     3901 catcatgtct tcacgagctg atcatacgga gtcagtcccg gctcttgctt ccgcgcgact
     3961 tcatagagct gttttagctc ttccgcggcc ttgtcgaacg ccatcagaag ctcctggtcg
     4021 tcgggaagaa gttcgtccag ttggccaccg accgccgcaa cgattcgctc tgcctcggca
     4081 tgaacggcct gaatggacat catgagggtg ctgccggaga tgttcacttc tgtcccttcg
     4141 attcggcgag gtacttgacc agggcgcacc gcgcgaggtc gtcgagcgct cccttgtagg
     4201 cgtcgagccg catgatgccg gccctcggtt cgatctgata gtggtttgcc tggaagcgct
     4261 tgttgtagtc cgtgaagcgg acgacgatgg acgcagggac ttccgttccc tccggaatga
     4321 cgaacgacca ccagaacttg ctcgggaaga cgttctcgcg atcgaagagc gacgtgccct
     4381 tgaaaggcgc aaccatcatc tggccgtcct tccccggaaa gggatgcgtg tcggcggggc
     4441 gcacgcgctg cacggagcct tcgggcgtgt tctgggtgta gggcttgtcc tcgaatgagg
     4501 gatggagcac ggccggcgct ggtgcgtcgc cgactatcgg gccgtcaggg aagtcctctc
     4561 ggcggatact tctgtacagg cgtgcggatg tggtcgtcat ggcgaaggcg aaggacacag
     4621 gcgtcgaatg atgccgcgac aacgcagccg cgtccctccc cacgacgagg ggttctcagc
     4681 caacgccgga cgcgcccagg agcgagaccc gcacccaggc gccactgctg actttatgac
     4741 cggaaacgaa aagaccgcca cgcgggcggt ccttcgaggg gtgttaggtg acgagcctga
     4801 ccccggcact ggtcacaaca gttagggctg cttcgttccc gacctgaccc ggttggccgt
     4861 gcttccatgc gaggaggccc gtcgctgcgt attgtaggcg atctcgcggc gctccacggc
     4921 cgggcagaaa gaaaagcgcc tcgcgcgcgg ccatgggccc tcgggcgagg cgccgtgcgc
     4981 atccgagcgc tcgggcgtgt tcagcgcagt tgcaggtcgt cgtcgccgaa gcgcacgccg
     5041 atgggctcga tgagaagctg cttcgggccg tcctcgacgc ggccggcgat ccacgcgccg
     5101 atgccgaggt cctgggcgat cttcaccgcc tgctccgcct ggccggccgg catgaagagg
     5161 gcgaagccgg cgcccatgtt gagcgtcgag taggcctcgc ggtcgtcctg cttcgcgtgc
     5221 tgctggatga acttgagcac cggcggcacc ggcggcacct gcgtgatgcg gtaggtgaag
     5281 ggcttcggat ggcggagcag cttgcgccag ccgtggcccg tgacgttggc gctgtagtgc
     5341 accgtcaggc cggccttggc gaggccttcg gtgacgggcg agtacagcgt cgtcggcgca
     5401 agcagcgcct cgccgtacga cagtcccggc tcgaccggcg tcatgtagcc gtgcggcagg
     5461 cgctcgacga gcttgcgcgc gaggctcagg ccgttggcgt ggatcccgct cgacgcgagt
     5521 agcacgatgg cgtcgccggc ctgcagctcg tcgccgaccg acaggcgcga cttcgggctg
     5581 acgatgccgg tgcacgacga ggcgagatcg atgcggccgc cctcgacgat gcccgcgagc
     5641 gccggcgtct cgccgccgcc ccaggcgacg ccgcaggtgt cgcaggccgc cttccagccg
     5701 tccacgagtg ccttggcccg cagcttgtcg ccgaaccagt cgctgccgcc ggcggcccag
     5761 taggcctgca cgaccagcgg cgtcgcgccg acggtgatga ggtcgttgat cgacatcgcg
     5821 atcgtgtcct gggcgatgcc cgcgtagaag ctgcggccgg tgagctcggc catctcgtcg
     5881 gcaaccagcg ccttcgatcc caggcattcg acgatgctgg cgaggtagaa cgggccgacg
     5941 tcgacgacgt aggccgactc gccgcgcgag gcggcgactt ccgagaaacc gtggccggcg
     6001 agggcgcccg ccgtcgcggc ggcggcgcgc tgggcggcga ccttcagcgg gtcgatcagg
     6061 tcatagttga cgccggcctg ctcgtagctg agcgtgtccg ggtggctgga agaggaatgc
     6121 gagctcatcc cgggattatc ggtcacgccg atggggccgg atccggcccg tgcgccatcg
     6181 gtagaatcgg ctcgatgagt tacgtcgtcc tcgcacgcaa gtaccgcccg cgccgcttcg
     6241 agcagatggt ggggcaggac cacgtcgtgc gcgcgctgac gcacgcgctc gagcagggcc
     6301 gcctgcacca cgcctacctg ttcaccggca cgcgcggcat cggcaagacg acggtctcgc
     6361 gcatcctcgc caagagcctg aactgcaccg gcccggacgg ccagggcggc atcacggccc
     6421 acccctgcgg ccagtgcacg gcctgcacgg agatcgacgc cgaccgctac atcgactacg
     6481 tcgagctcga cgccgcgtcc aatcggtcga tcgacgagat ccgcgacctc atcgagcgcg
     6541 ccgcttacaa gccgagcgtg ggccgctaca aggtcttcat gatcgacgag gcgcaccagc
     6601 tcacgaagga cgccttcaac gcgctgctga agacgctgga ggagccgccc gagtacctga
     6661 agttcgtgct ggcga
//
LOCUS       DASVLN010000677         7964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_144574, whole genome shotgun sequence.
ACCESSION   DASVLN010000677 DASVLN010000000
VERSION     DASVLN010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144574"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VF453_16040"
     CDS             complement(<1..178)
                     /locus_tag="VF453_16040"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX7689225.1"
                     /translation="MTLYIGLMSGTSMDGVDGVLVDFAAGGAGLRVLADAHATMPEAL
                     GGELLALNAAGADEL"
     gene            complement(264..1664)
                     /locus_tag="VF453_16045"
     CDS             complement(264..1664)
                     /locus_tag="VF453_16045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7689226.1"
                     /translation="MDPLSPVEQAVDTAVQSLLGRVARWAHAHPRKLATGAATLLGGF
                     AVAAFGIAPMAPDAADLPRRTITEAYAPENLDAQLDALAAQHLALSRSDVTRGNDTAA
                     TLSKRLGVTDAEFAAFLQSDPTSRKLWQGRAGKLVSATTDDDGRLTRLVARYPAERDD
                     QFSPHFTRLTIARGEHGFASKVETVPFGTTTRLASGTVRTSLFAATDEASIPDPVATQ
                     MADMFSTDVDFRRELRKGDTFSVVYEALTADGEPVPWNQGAGRVLAAEFVNNGHAFSS
                     VWFQDASGKGAYFDMDGRSKHRAFLASPLAFTRITSGFSMRFHPILKQWKRHEGVDYG
                     APIGTPVHVVGDGVVAFAGVQNGYGNVIQVQHSDNRMTVYAHLSKIDVVRGQHVSQGD
                     HIGDVGMTGWATGPHLHFEFRVAGVVKDPLTIAKSAETLTLDDASRPRFASLAGELKS
                     ELGVAETLNGRVGRID"
     gene            1993..3231
                     /gene="tyrS"
                     /locus_tag="VF453_16050"
     CDS             1993..3231
                     /gene="tyrS"
                     /locus_tag="VF453_16050"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX7689227.1"
                     /translation="MNDLTAAPAHPVTDRVREALAVSLRGCDELLPESDWVKKLARSE
                     ATGTPLRIKLGLDPTAPDIHVGHTVVLNKMRQLQDLGHTVIFLIGDFTSLIGDPSGRN
                     STRPPLTHEQIKANAETYYRQASLVLDSSRTEIRYNSEWSDPLGARGMIQLAAKYTVA
                     RMMERDDFTKRFTAQTPISVHEFLYPLMQGYDSVALRSDLELGGTDQKFNLLVGRHLQ
                     QEYGQEPQCILTMPLLEGLDGVEKMSKSKNNYVGIAEPANTMYAKILSISDVQMWKWF
                     TLLSFRSEAEIAKLKAEVDGGRNPMEAKVMLAKEITARFHSAAAADAAEADFRNRSRG
                     GIPDDVKDVALAGAPMPIAALLRQAGLVESNSEGNRMIDQGGVRIDQVVVSDKGLKVD
                     AGTYVFQVGKRKFARVTLSA"
     gene            complement(3235..3912)
                     /locus_tag="VF453_16055"
     CDS             complement(3235..3912)
                     /locus_tag="VF453_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689228.1"
                     /translation="MPDPQRALVIATPSIDGNVSAHYTGSLVRSLGLLRQRGVHAEIH
                     FEIGNSLIADARNKLVSKFLASGASDLLFIDADLSWNAEDLARLVSYPVPMVAGVYQR
                     KSRTKLDFSVKFGPAIVRDAHGLMAVERVGTGFMRLRRDCIERMVAAHPHLKLRNPTD
                     PADEHLYALFDTVVDGGQFIGEDFAFCDRWRAIGGEVLIDPAIAFAHHGSAAYDEPLL
                     KYLQKNG"
     gene            4001..4921
                     /locus_tag="VF453_16060"
     CDS             4001..4921
                     /locus_tag="VF453_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689229.1"
                     /translation="MTSPDPAATALAAVLQRADALLSRLETLLPAAAAAPDWDASIAF
                     RYRRRSAGLLGAAGGVLEPVRHVAPIALASLKEVDAQKERLRANTAQFVAGRPANNVL
                     LTGARGTGKSSLIKACLHAFAPQGLRLIEVDKADLVDLPEIVALIAGRPERFVVYCDD
                     LSFDEGEAGYKALKSMLDGTVSAQSDNVLVYATSNRRHLLPETMKENLAATYDEDGEL
                     HPGEAVEEKISLSERFGLWISFYPFSQNEYLAIVAQWLRHFGLGDDAAAALRGEALVW
                     ALERGSRSGRVAYQFARDRAGRLAGEGASA"
     gene            4918..5433
                     /locus_tag="VF453_16065"
     CDS             4918..5433
                     /locus_tag="VF453_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7689230.1"
                     /translation="MSGGGRPVEVSDADRRAHTEVVESEDAPSRYGERPVTEVAVGVL
                     VDADGRFLLTSRPAGKVYAGYWEFPGGKLESGETVEQALRRELHEEIGITIAAAVPWH
                     VLMMDYPHARVRLNFCKVFEWTGRFEMREGQQMAWERLPVASGPVLPGTIPVLEWFAK
                     EQGFEGPTHRG"
     gene            5560..5949
                     /gene="hisI"
                     /locus_tag="VF453_16070"
     CDS             5560..5949
                     /gene="hisI"
                     /locus_tag="VF453_16070"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX7689231.1"
                     /translation="MTWLDDVKWDRDGLVPVIAQERGTGDVLMFAWMNREALERTADS
                     GRAVYWSRSRNKLWAKGEESGHVQQVHALRLDCDNDVVLMEVTQLGHEPGIACHTGRH
                     SCFFQELRDGAWVAVEPVLKDPGSIYK"
     gene            5946..6317
                     /locus_tag="VF453_16075"
     CDS             5946..6317
                     /locus_tag="VF453_16075"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX7689232.1"
                     /translation="MNGDDTLHRLAQVIESRKPAHGGDPAASYVARLFDKGTDAILKK
                     VGEEAVETVMAAKDGDPKKIVYEVADLWFHSMIALAHFGLAPEDVLAELRRREGLSGL
                     EEFALRKAGRRDAAEKKDGAP"
     gene            6379..6726
                     /locus_tag="VF453_16080"
     CDS             6379..6726
                     /locus_tag="VF453_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689233.1"
                     /translation="MSLVDQPGGPRTLTHVLYALHTVTWFSGGIFSVIAIIVNMVNYA
                     SLPDDFYRSHWRWQARTFWFALIAFLVTWPLWLLFVVPGWIAWTVVGLWYLYRCIRGW
                     INFNDGRAMPVPL"
     gene            6751..7098
                     /locus_tag="VF453_16085"
     CDS             6751..7098
                     /locus_tag="VF453_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX7689234.1"
                     /translation="MAHDPNCIFCKLVAGTIPSRKAYEDEEILVFHDIAPWAPVHLLL
                     IPKRHVPSMAEVTAADAPLLGRMMAMIPQLMLDHGASNGFRVIVNTGVDGGQEVPHLH
                     LHAIGGPRPWAKG"
     gene            7235..7486
                     /gene="tatA"
                     /locus_tag="VF453_16090"
     CDS             7235..7486
                     /gene="tatA"
                     /locus_tag="VF453_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEX7689235.1"
                     /translation="MGISTTHLIIFLVIIVLIFGTRKLKNIGTDLGAAVKGFKDGMKD
                     GEAAKSPEQEVAHPAQQVSGPAADKRPAGETIDVEATRR"
     gene            7516..>7964
                     /gene="tatB"
                     /locus_tag="VF453_16095"
     CDS             7516..>7964
                     /gene="tatB"
                     /locus_tag="VF453_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX7689236.1"
                     /translation="MFDIGVTKLAIVGGIALIVIGPERLPRVARLVGTMLGRAQRYVA
                     DVKAEVNRSIELEELGKMKSHFEAAAREVQDTVNEHVHGVRAAIEDRAPALGAFGDEA
                     AAMRPMPVYKPPRKNWRVKRAAVPTWYKQRAGVRTHAQSGAARVARFR"
BASE COUNT         1243 a         2725 c         2862 g         1134 t
ORIGIN      
        1 gcagctcgtc cgcgccggcg gcgttcagcg ccagcagctc cccgcccagc gcctcgggca
       61 tggtcgcgtg ggcgtcggcg agcacgcgca gtcccgcccc gccggccgcg aaatcgacga
      121 gcacgccgtc gacgccgtcc atcgacgtgc cgctcatcag tccgatgtag agggtcatga
      181 tgtgacgacg cgccgcggag tcggcccggc ggcgcgtcgg tggttccggg acaggcccga
      241 tcgtagccgg gcggcccggt gcgtcagtcg atgcggccga cgcgtccgtt gagcgtctcg
      301 gccacgccga gctccgactt cagctccccg gccagcgagg cgaagcgcgg ccgcgaggcg
      361 tcgtcgagcg tgagcgtctc ggccgacttg gcgatggtga gcggatcctt gacgacgccg
      421 gcgacgcgga actcgaaatg caggtgcggg cccgtggccc agccggtcat gccgacgtcg
      481 ccgatgtgat cgccctgcga cacatgctgg ccgcgcacga cgtcgatctt gctcaggtgc
      541 gcgtagaccg tcatgcgatt gtccgagtgc tgcacctgga tcacgttgcc gtagccgttc
      601 tgcacgcccg cgaacgcgac gacgccgtcg cccacgacat gcaccggcgt gccgatcggg
      661 gcgccgtagt cgacgccctc gtggcgcttc cactgcttga ggatcgggtg gaagcgcatc
      721 gagaaccccg acgtgatgcg cgtgaaggcc agggggctcg cgaggaacgc ccggtgcttg
      781 ctgcggccgt ccatgtcgaa ataggcgccc ttgccgctgg cgtcctggaa ccagaccgag
      841 gagaacgcgt ggccgttgtt gacgaactcc gcggccagca cgcggcccgc gccctggttc
      901 cagggcaccg gctcgccgtc ggcggtcagc gcctcgtaga cgacgctgaa cgtgtcgccc
      961 ttgcgcagct cgcggcggaa gtcgacgtcg gtcgagaaca tgtccgccat ctgggtggcg
     1021 accggatcgg ggatgctcgc ctcgtccgtc gccgcgaaca gcgaggtgcg cacggtaccc
     1081 gaggcgaggc gcgtcgtcgt gccgaacggc acggtctcga ccttgctcgc gaagccgtgc
     1141 tcgccgcggg cgatggtcag gcgcgtgaag tggggcgaga actggtcgtc gcgctccgcc
     1201 gggtagcgag cgacgagccg ggtcagccgg ccatcgtcgt cggtcgtagc cgagacgagc
     1261 ttgcccgcac ggccctgcca gagcttgcgc gacgtgggat cggactgcag gaaggcggcg
     1321 aactcggcgt ccgtgacacc cagccgcttg gacagcgtcg ctgcggtgtc gttgccgcgc
     1381 gtgacgtcgc tgcgcgacag cgcgaggtgc tgcgcggcga gggcgtccag ctgggcgtcg
     1441 agattctcgg gcgcgtaggc ctcggtgatc gtgcggcgcg gcaggtcggc cgcgtccggc
     1501 gccatcggcg cgatgccgaa ggcggcgacg gcgaagccac cgagcagggt ggcggcgccg
     1561 gtggcaagct tgcgggggtg ggcatgcgcc cagcgcgcga cgcggccgag cagggactgg
     1621 acggcggtgt cgaccgcctg ttcaacgggg ctcaggggat ccaacgacga tctcttcagc
     1681 ggggggagcc gggtgtcgcg attgccgcga agccctctcc ggttttcagg aatccgcacc
     1741 ctcgacaggc atcacctggc cgtgcgcgcg cttagaattc agtgacggat cgccgctttt
     1801 gttgatgaaa gacaacggaa gcggcaaaga cactttgatt tagctcgccg gatcggcggc
     1861 tccatggtag agccgcgatc gatccgcgcg agcgactata gcagtgcgtt gtaaagtcgc
     1921 tcccccgaaa gagggacttt acacaccgtt ttcctcgtgg tttacccgcg ccgccgccga
     1981 cttcccctgc cgatgaacga cctcaccgcc gcccccgccc atcccgtcac cgaccgcgtg
     2041 cgcgaggcgc tcgcggtcag cctgcgcggc tgcgacgaac tgctgcccga gtcggactgg
     2101 gtgaagaagc tggcgcgatc ggaagccacc ggcacgccgc tgcgcatcaa gctgggcctg
     2161 gatccgacgg cgcccgacat ccacgtcggc cacaccgtcg tgctcaacaa gatgcgccag
     2221 ctgcaggacc tggggcacac ggtgatcttc ctgatcgggg acttcacgtc cctgatcggc
     2281 gacccgtcgg gccgcaactc gacccgcccg ccgctgacgc acgagcagat caaggccaac
     2341 gccgagacct actaccggca ggcgagcctg gtgctcgact cgtcgaggac cgagatccgc
     2401 tacaacagcg agtggagcga cccgctcggc gcccgcggca tgatccagct cgccgccaag
     2461 tacaccgtcg cgcgcatgat ggagcgcgac gacttcacca agcgcttcac ggcccagacg
     2521 cccatcagcg tgcacgagtt cctctacccg ctgatgcagg gctacgactc ggtggcgctc
     2581 aggagcgacc tcgagctcgg cggcaccgac cagaagttca acctgctcgt gggccgccac
     2641 ctgcagcagg aatacggcca ggagccgcag tgcatcctca cgatgccgct gctggagggg
     2701 ctggacggcg tcgagaagat gtcgaagtcc aagaacaact acgtgggcat cgcggagccc
     2761 gccaacacga tgtacgcgaa gatcctgtcg atcagcgacg tgcagatgtg gaagtggttc
     2821 acgttgctga gcttccgctc cgaggccgag atcgcgaagc tgaaggcgga ggtcgacggc
     2881 gggcgcaacc cgatggaggc caaggtgatg ctcgccaagg agatcacggc gcgcttccat
     2941 tcggcggcgg cggccgacgc ggccgaggcg gacttccgca accgctcgcg cggcggcatc
     3001 cccgacgacg tgaaggacgt cgcgctggcc ggcgcgccga tgccgatcgc cgcgctgctc
     3061 agacaggcgg ggctcgtcga gtccaacagc gagggcaacc gcatgatcga ccagggcggc
     3121 gtgcgcatcg accaggtcgt cgtcagcgac aagggactca aggtcgacgc cggcacgtac
     3181 gtgttccagg tcggcaagcg caagttcgcg cgcgtgacgc tcagcgcctg agcgtcagcc
     3241 gttcttctgc aggtacttca gcagcggctc gtcgtaggcc gcgctgccat ggtgcgcgaa
     3301 ggcgatcgca ggatcgatca gcacctcgcc gccgatcgcg cgccagcggt cgcagaaggc
     3361 gaagtcctcg ccgatgaact ggccgccgtc gacgacggtg tcgaacagcg cgtacaggtg
     3421 ttcgtcggcc ggatcggtcg gattgcgcag cttgaggtgc ggatgggctg cgaccatgcg
     3481 ctcgatgcag tcgcggcgca gccgcatgaa gccggtgccg acgcgctcga cggccatcag
     3541 gccgtgggcg tcgcggacga tggccgggcc gaacttgacc gagaagtcga gcttcgtgcg
     3601 gctcttgcgc tggtagacgc cggccaccat cggcaccggg taggagacca ggcgcgcaag
     3661 gtcctccgcg ttccaagaga ggtcggcatc gatgaacagc aggtcgctcg cacccgaggc
     3721 gaggaacttg gacaccagct tgttgcgcgc gtcggcgatc aggctgttgc cgatctcgaa
     3781 gtggatctcc gcgtgcacgc cgcgctggcg cagcaggccc agggagcgca ccagcgagcc
     3841 ggtgtagtgc gcgctgacgt tgccgtcgat ggatggcgtg gcgatgacga gggcgcgctg
     3901 cgggtcgggc atcggggagg tcgcgggcgg gaggatgggt tcatcgtacg cgaagggcga
     3961 gcgcctcggt cggcacccgc gcctcgctag acttccgccc atgacctcgc ccgatcccgc
     4021 cgcgaccgcg ctcgcggccg tgctccagcg cgccgacgcg ctgctctccc gcctcgagac
     4081 cctgctgcct gcggccgccg ccgcgccgga ctgggacgcc tcgatcgcct tccgctatcg
     4141 ccgccgcagc gcgggcctgc tgggcgccgc cggcggcgtc ctcgagccgg tgcgccacgt
     4201 cgcgccgatc gcgctcgcga gcctgaagga ggtcgacgcg cagaaggagc gcttgcgcgc
     4261 gaacacggcg cagttcgtcg ccgggcggcc cgcgaacaac gtgctgctca cgggcgcgcg
     4321 cggcaccggc aagagctcgc tgatcaaggc ctgcctgcac gcgttcgcgc cgcagggact
     4381 gcggctgatc gaggtggaca aggccgacct cgtcgacctg cccgagatcg tcgccctgat
     4441 cgccggccgc ccggagcgct tcgtcgtcta ctgcgacgac ctgagcttcg acgagggcga
     4501 agccggctac aaggcgctga agtcgatgct ggacggcacc gtctcggcgc agtcggacaa
     4561 cgtgctcgtc tacgcgacgt cgaaccgccg ccatctcttg cccgagacga tgaaggagaa
     4621 cctcgccgcg acctacgacg aggacggcga gctgcatccc ggcgaggcgg tggaggagaa
     4681 gatctcgctg tccgagcgct tcggcctgtg gatcagcttc tatccgttca gccagaacga
     4741 gtacctcgcg atcgtcgcgc agtggctgcg gcatttcggc ctcggcgacg acgcggccgc
     4801 cgcgctgcgc ggcgaggcgc tcgtgtgggc gctggagcgc ggctcgcgat cgggccgcgt
     4861 cgcgtaccag ttcgcgcgcg accgcgccgg gcggctcgcg ggcgaagggg cgtcggcatg
     4921 agcggcggcg gcaggccggt cgaggtcagc gacgccgatc ggcgcgcgca cacggaggtc
     4981 gtcgagtcgg aggacgcgcc ctcgcgctac ggcgagcggc cggtgacgga ggtcgccgtg
     5041 ggcgtgctgg tcgacgccga cggccgcttc ctgctcacct cgcggcccgc gggcaaggtc
     5101 tacgcgggct actgggagtt cccgggcggc aagctcgagt ccggcgagac ggtggagcag
     5161 gcgctgcgcc gcgagctgca cgaggagatc ggcatcacca tcgccgcggc cgtgccctgg
     5221 cacgtgctga tgatggacta cccgcacgcg cgcgtgcgcc tgaacttctg caaggtgttc
     5281 gagtggacgg gccgcttcga gatgcgcgag ggccagcaga tggcctggga gcgcctgccg
     5341 gtcgcgagcg ggccggtgct gccggggacg atccccgtgc tggagtggtt cgcgaaggaa
     5401 cagggcttcg aaggtccgac gcaccgtggc tgacgactac actcgcgaca tgaaagctac
     5461 gcagtcgaag cgctcgcggg gcgagcgccg ggccgccccg agccggcccg cgggcccctc
     5521 ggggggcgca gcgccgtact cggcgcggga ggggctccag tgacctggct cgacgacgtc
     5581 aaatgggatc gcgacggcct cgtgccggtg atcgcgcagg agcgcggcac cggcgacgtg
     5641 ctgatgttcg cgtggatgaa ccgcgaggcg ctcgagcgca ccgccgacag cgggcgcgcg
     5701 gtctactgga gccgctcgcg caacaagctg tgggccaagg gcgaggagtc cgggcatgtg
     5761 cagcaggtgc acgcgctgcg gctggactgc gacaacgacg tcgtgctcat ggaggtgacg
     5821 cagctcggcc acgagcccgg catcgcctgc cacacgggtc gccacagctg cttcttccag
     5881 gagctgcgcg acggcgcctg ggtcgcggtc gagccggtgc tcaaggatcc ggggagcatc
     5941 tacaaatgaa cggcgacgac acgctccacc gccttgcgca ggtcatcgag tcgcgcaagc
     6001 cggcccacgg cggcgacccc gcggcgagct acgtcgcgcg cctgttcgac aagggcaccg
     6061 acgcgatcct caagaaggtc ggcgaggagg ccgtcgagac ggtgatggcg gccaaggacg
     6121 gcgacccgaa gaagatcgtc tacgaggtgg ccgacctctg gttccactcg atgatcgcgc
     6181 tcgcgcactt cggcctcgcg ccggaggacg tgctcgccga gctgcgccgt cgcgagggcc
     6241 tgtcgggcct cgaggagttc gcgctgcgca aggcaggccg gcgcgacgcg gcggagaaga
     6301 aggacggtgc gccatgagcc cccgcccggc cgctccggag ggggctcgcg ccgaagtccg
     6361 caggatggaa gttgccatat gagcctcgtc gaccagcccg gcggcccgcg cacgctcacc
     6421 cacgtgctct acgcgctgca caccgtcacg tggttctcgg gcggcatctt ctcggtgatc
     6481 gcgatcatcg tgaacatggt gaactacgcc tcgctgccgg acgacttcta ccgcagccac
     6541 tggcgctggc aggcgcgcac gttctggttc gcgctgatcg cgttcctcgt cacctggccg
     6601 ctgtggctgc tgttcgtggt gccgggctgg atcgcgtgga cggtggtcgg cctgtggtac
     6661 ctctaccgct gcatccgcgg ctggatcaac ttcaacgacg gccgggcgat gcccgtgcct
     6721 ctctgagcga acctacgagc ggagcccccg atggcgcacg acccgaactg catcttctgc
     6781 aagctggtgg cgggcacgat cccgtcgcgc aaggcctacg aggacgagga gatcctggtc
     6841 ttccacgaca tcgcgccctg ggcgccggtg cacctgctgc tgatccccaa gcgccacgtc
     6901 ccctcgatgg ccgaggtgac cgcggccgac gcgccgctgc tcggccgcat gatggcgatg
     6961 atcccgcagc tgatgctgga ccacggcgcg tccaacggct tccgcgtgat cgtcaacacc
     7021 ggcgtggacg gcgggcagga ggtgccgcac ctgcacctgc acgcgatcgg cgggccgcgg
     7081 ccgtgggcca agggctgagc gccatcgccc cgtgcgagcc gcgaacgtga ccgaatgcct
     7141 gcccgaccgg tgcctcgggc acccggtcaa ctagaatgtc gacttcgggc gtccgcgcgg
     7201 ccgcccggtg cagcaagacc tgaaggagcc cgccatgggt atctcgacca ctcacctgat
     7261 catcttcctc gtcatcatcg tgctcatctt cggcacgagg aagctcaaga acatcggcac
     7321 cgacctcggc gcggccgtca agggtttcaa ggacggcatg aaggacggcg aagccgccaa
     7381 gtcgcccgag caggaggtcg cgcacccggc ccagcaggtc agcggcccgg ccgccgacaa
     7441 gcgccccgcc ggcgagacga tcgacgtcga ggccacgcgc cgctgacgcc cggccacgcg
     7501 acgcgttccc gctgaatgtt cgacatcggc gtcaccaagc tcgcgatcgt cggcggcatc
     7561 gcgctgatcg tcatcggccc cgagcgcctg ccgcgcgtcg cccgcttggt cggcacgatg
     7621 ctgggccggg cgcagcgcta cgtcgcggac gtgaaggccg aggtcaaccg ctccatcgag
     7681 ctcgaggagc tcggcaagat gaagtcgcac ttcgaggccg ccgcgcgcga ggtgcaggac
     7741 accgtcaacg agcacgtgca cggcgtgcgc gcggcgatcg aggatcgtgc cccggcgctc
     7801 ggcgcgttcg gcgacgaggc ggcggcgatg cgaccgatgc ccgtctacaa gccgccgcgc
     7861 aagaactggc gcgtcaagcg tgccgccgtc ccgacctggt acaagcagcg cgccggcgtg
     7921 cgcacgcacg cgcagtcggg cgcggcgcgc gtggcgcgct tccg
//
LOCUS       DASVLN010000678         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_466314, whole genome shotgun sequence.
ACCESSION   DASVLN010000678 DASVLN010000000
VERSION     DASVLN010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466314"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VF453_16100"
     CDS             complement(<1..532)
                     /locus_tag="VF453_16100"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEX7689237.1"
                     /translation="MLHVHFSNRFERLAEALARRLAQAPAGGDPFAADEVVVPSAAVT
                     RRLILELARRHGICANVRFSYLAQWLWRQGAALMPTLPAESPFDADVLAWRILAAFED
                     EAWASAQPRLADWLAGADAAMRHELARRVAALFDQYLTYRPEWLQDWVEERCVDLGAA
                     SADAALDERWQAALWRR"
     gene            complement(628..1410)
                     /locus_tag="VF453_16105"
     CDS             complement(628..1410)
                     /locus_tag="VF453_16105"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX7689238.1"
                     /translation="MRPLTAKSHLLAALAITAAAVAAAFAIPASPAQATTITINGDGG
                     GFVAGSGHEVAAARRVGAFTALRVEGSVDVTAHPGAAGVVVHADDNIEPLIETVVEGD
                     TLVVRLRRNASFRTSDKVSVDVAFASLSETQQHGSGDLHVTGVSGPRLASSIHGSGDL
                     EIDGAQVGSFSLAIAGSGDARLSGHADEARFKVDGSGDVHAAHFPARRVEVDVAGSGD
                     AEVNATEAIDARVAGSGDVVYEGHPHDVSRRVAGSGSIEARH"
BASE COUNT          198 a          540 c          612 g          189 t
ORIGIN      
        1 ggcgccgcca cagcgcggcc tgccagcgct cgtcgagcgc ggcgtccgcg gacgccgcgc
       61 cgaggtcgac gcaacgctcc tcgacccagt cctgcagcca ttccggccgg taggtcaggt
      121 actggtcgaa cagcgcggcc acgcggcgcg cgagctcgtg gcgcatggcg gcgtcggcgc
      181 cggcgagcca gtcggccagc cgcggctgcg cggacgccca ggcctcgtcc tcgaacgcgg
      241 cgaggatgcg ccatgcgagc acgtcggcgt cgaagggcga ttcggccggc agcgtcggca
      301 tcagcgccgc accctggcgc cacagccact gcgcgaggta ggagaagcgc acgttggcgc
      361 aaatgccgtg gcggcgcgcc agctccagga tcagtcgtcg cgtgacggcc gcgctcggaa
      421 ccaccacctc gtcggccgcg aacgggtcgc cgcccgccgg cgcctgcgcg aggcgccggg
      481 cgagcgcctc ggcgagccgt tcgaagcggt tggagaagtg gacgtgcagc atcggggcgg
      541 agcgtgggcg gccattgtgc cgcgcggctc cgcaaccccg agcttggggg cgggcaggct
      601 cactgggtga gccagtccgt cactgagtca gtgccgcgcc tcgatcgagc cggagccggc
      661 gacgcggcgc gagacgtcgt gcggatggcc ttcgtagacc acgtcgcccg agccggcgac
      721 gcgggcgtcg atcgcctcgg tggcgttgac ctcggcgtcg cccgagccgg cgacgtcgac
      781 ctcgacgcgg cgggcgggga agtgtgcggc gtggacgtcg cccgagccat cgaccttgaa
      841 gcgggcctcg tcggcgtggc cgctgagcct ggcgtcgccg gagccggcga tcgcgagcga
      901 gaagctgccg acctgggcgc cgtcgatctc caggtcgccc gagccgtgga tggaggatgc
      961 cagacgcggc ccgctgacgc cggtgacgtg caggtcgccg ctgccgtgct gctgggtctc
     1021 cgacagcgac gcgaacgcga cgtccaccga caccttgtcg ctggtgcgga agctcgcgtt
     1081 cctgcgcagg cgcacgacga gggtgtcgcc ctcgaccacg gtctcgatca gcggctcgat
     1141 gttgtcgtct gcatgcacga cgacgccggc cgcgcccggg tgggccgtga cgtcgacgga
     1201 gccctcgacg cgcagcgcgg tgaacgcgcc gacgcggcgc gcggccgcga cctcgtggcc
     1261 cgagccggcg acgaagccgc cgccgtcgcc gttgatggtg atcgtcgtcg cctgcgccgg
     1321 cgaggcgggg atcgcgaacg cggccgcgac cgccgcggcg gtgatggcca gggcggcgag
     1381 caggtgggac ttggcggtca gggggcgcat ggcggcgggc tcctcgggac ttctgattcg
     1441 atggagcgca tgctaggcgg ccatcgcggc caggtcgcgg gcggggcgac gagtcgcgcc
     1501 gcgcgcgcga ccaggtgcgg ttccgcgcga cgaacggcg
//
LOCUS       DASVLN010000679         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_195962, whole genome shotgun sequence.
ACCESSION   DASVLN010000679 DASVLN010000000
VERSION     DASVLN010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195962"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..944)
                     /locus_tag="VF453_16110"
     CDS             complement(24..944)
                     /locus_tag="VF453_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX7689239.1"
                     /translation="MFSHNKRLQYTVRVSETNPGLANLMLEQFGGPQGELAAACRYFT
                     QYLAEEDPGRKDMLIDIATEELSHLEMIGTIVGMLNRGAKGRLSEATESEAELYRSIN
                     GAGNDSHVTQVLYGGGPALVNSAGVPWTAAYVDSIGDPTCDLRSNIAAEARAKIVYER
                     LINLTDDPGVKEALGFLMTREVSHQLSFEKALYSIQPNFPPGKMPPKPEFARVYFNMS
                     RGRATSERDGQGGGERGPWNASPDFDYREGEPAVDGGSGGFDVAMKPEEQAALDAMAL
                     RLKSELDSQPLTGAELGSGVALPDGDGAAA"
     gene            complement(1076..1621)
                     /locus_tag="VF453_16115"
     CDS             complement(1076..1621)
                     /locus_tag="VF453_16115"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7689240.1"
                     /translation="MSILDKAIAAVTPTEKPEERAQARAKAYELARTSPWLGRVLDQH
                     REIESAFEAVAAATHADSRRLAQKRLAVLLTGHSLAEEAVLYPAMALGDQKAHATIAL
                     TEHSGVKVNLSALETLDPMSQDYLDKLEHVKNAVTHHVYEEESNWYPELARDTPPGDQ
                     MRLDMRFTEEFERYMGADAAA"
BASE COUNT          246 a          720 c          709 g          339 t
ORIGIN      
        1 cgtcggccgc cgggccctgc ggcctaggcg gccgcgccgt cgccatcggg cagcgccacg
       61 cccgagccca gctccgcgcc ggtgagcggc tgcgagtcga gctccgactt cagccgcagc
      121 gccatcgcgt ccagcgccgc ctgctcctcg ggcttcatcg cgacgtcgaa gcccccgctg
      181 ccgccgtcga ccgccggctc gccctcgcgg tagtcgaagt cgggcgaggc gttccacggg
      241 ccgcgttcac cgccgccctg gccatcgcgc tcgctggtcg cgcggccgcg cgacatgttg
      301 aagtacacgc gcgcgaactc cggcttgggc ggcatcttgc ccggcgggaa gttcggctgg
      361 atcgagtaca gcgccttctc gaacgacagc tggtgcgaga cctcgcgcgt catcaggaag
      421 ccgagcgcct ccttcacgcc ggggtcgtcc gtgaggttga tcaggcgctc gtagacgatc
      481 ttggcgcgcg cctccgcggc gatgttggag cgcaggtcgc aggtcgggtc gccgatcgag
      541 tcgacgtagg ccgccgtcca gggcacgccc gcggagttga ccagcgccgg cccgccgccg
      601 tagagcacct gcgtgacgtg cgagtcgttg cccgcgccgt tgatcgagcg gtagagctcg
      661 gcctccgact cggtcgcctc ggacagccgg cccttcgcgc cgcgattgag catgccgacg
      721 atggtgccga tcatctcgag gtggctcagc tcctcggtcg cgatgtcgat caacatgtcc
      781 ttgcggccgg gatcctcctc cgcgaggtac tgcgtgaagt agcggcacgc ggccgcgagc
      841 tcgccctgcg ggccgccgaa ctgttcgagc atgaggttgg cgagaccggg attggtctcg
      901 ctcacccgca ccgtgtattg cagacgcttg ttgtgagaga acatgcgtgg gaccttcctg
      961 cgccgatgtc gatcggcatt gcggttgacg aatggcgacg cgacgcggcg cacgaggacg
     1021 ttgcaggcaa cgcgcattcc ccgccgcgca gcggctccgt gcgcccgcgc gcgcctcacg
     1081 ctgccgcgtc ggcgcccatg tagcgctcga actcctcggt gaagcgcatg tccaggcgca
     1141 tctggtcgcc cggtggcgta tcgcgcgcca gctccgggta ccagttgctc tcctcctcgt
     1201 agacgtggtg cgtcaccgcg ttcttcacgt gctcgagctt gtcgaggtag tcctggctca
     1261 tcgggtcgag ggtctcgagc gccgacaggt tgaccttgac gccgctgtgc tcggtgagcg
     1321 cgatcgtcgc gtgcgccttc tggtcgccga gcgccatcgc cgggtagagc acggcctcct
     1381 cggcaagcga gtggccggtc agcagcaccg caaggcgctt ctgggcgagg cgtcgcgagt
     1441 cggcatgcgt cgcggcggcc accgcctcga acgccgattc gatctcgcga tgctggtcga
     1501 gcacgcgccc gagccagggg ctggtgcgcg cgagctcgta ggccttggcg cgcgcctgcg
     1561 cacgctcctc gggcttctcg gtgggggtga cggccgcgat ggccttgtcg aggatgctca
     1621 tggtcgctgc tccttcatcc gccgtggtca gcgcgtgccg ccgctggtgc cttggttgcc
     1681 gccaccgccc gagcccgggg ccacggccgt cgccgcctgc gcgagggacg ccagcaccgc
     1741 gacgccgtat cgaatcactt gtctcattca cgactccttg aggatgtgtt cgccgccgtg
     1801 atggcgacgg gactcgccga ggcaacgcct gtgccgcgcc gcgtagccgc gcttccatcg
     1861 gggccgtccg gcgcgggccc gtgggaatgc cgatcgcgtt gtgccgggcc tgccgcacga
     1921 ttgccggcac cttcgggttc tgcggctttg aagtcgaggc gcccgcgcgc gaccagcgtg
     1981 cgcagcaccg ccgccgcgcg cgcccgcatg cgcg
//
LOCUS       DASVLN010000680         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_410019, whole genome shotgun sequence.
ACCESSION   DASVLN010000680 DASVLN010000000
VERSION     DASVLN010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410019"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VF453_16120"
     CDS             complement(<1..624)
                     /locus_tag="VF453_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7689241.1"
                     /translation="MNTPRTSGAAGARVDWLPLVTVALGFVMAMLDVTVVNVALGDVR
                     ADLSASLSTLVWVVDGYTLSFAALLLAGGALANRFGARAVYVVGLALFVVASILCGAA
                     PGAAALVAARLLQGCAAALFIPSSLGLLTLAYPDHAVRARMLGLWAAVVSMSAASGPL
                     VGGVVVTWLGWRAIFLLNVPIGLAGIWLARRHLVATTGRPQALNLAAH"
     gene            740..1693
                     /locus_tag="VF453_16125"
     CDS             740..1693
                     /locus_tag="VF453_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7689242.1"
                     /translation="MDWDDARVFLAIYRQGTLRGAADALRIDQATAGRRLAALEGALG
                     ARLFLRTPRGYTPTAAGEQAFEAMARMEEGAHALLRRMQGLDDRLEGTVSLACTDTAA
                     TVFLMPALRRLHDRHPDILVRLVASTRVSSLTHREADLAVRSVRPTEPDLIVRHLGRR
                     RLGLYAASDYLAARGSPEPGTGLAGHDTVIYAPEVYPGQREFLCTEPVGAARVALEVN
                     TGMMMAEAVAAGIGIGELPHHLAALYPGLVRIWPDREDPYDMWLVMHDDLNRSARVRA
                     VAEAIVEAFEEEAAAAAVSPGPRSRPTRRRAARRRAAWRRR"
     gene            complement(1620..>1862)
                     /locus_tag="VF453_16130"
     CDS             complement(1620..>1862)
                     /locus_tag="VF453_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7689243.1"
                     /translation="GVLRFNQYDQVIQAALAGQGVALGRFPLLQPLVDDGRLVLAAPD
                     EPPLETRYAYWLITADATPRDAVRRVAEWVESEARD"
BASE COUNT          282 a          682 c          691 g          207 t
ORIGIN      
        1 gtgggcggcc aggttgagcg cctgcggccg gcccgtcgtc gcgacgaggt gccgccgcgc
       61 cagccagatg ccggccaggc cgatcggcac gttgagcagg aagatcgcgc gccagcccag
      121 ccacgtgacg acgaccccgc cgaccagcgg ccccgaggcg gccgacatcg acaccaccgc
      181 cgcccacagg cccagcatgc gggcacgcac ggcgtggtct gggtaggcga gcgtcagcag
      241 cccgagcgag ctcgggatga acagcgccgc cgcgcagccc tgcagcaggc gcgcggcgac
      301 cagcgcggcc gcgcccggtg ccgccccgca caggatcgac gccacgacga acagcgcgag
      361 gccgacgaca tagaccgccc gcgcgccgaa gcggttggcg agcgcgccgc cggccagcag
      421 caaggccgcg aacgacaggg tgtagccgtc gaccacccag accagcgtgg atagcgacgc
      481 cgacaggtcg gcgcggacgt cgccgagcgc gacgttgacg acggtcacgt cgagcatggc
      541 catcacgaag ccgagggcga cggtgaccag cggcagccag tcgacgcgcg cgccggcggc
      601 gccggaggtt cgaggtgtgt tcatgggaag tccagggtga agaggaacgc ctggatccat
      661 gctaagaatc gggcctcgcg aacggaagcc acgcggctgc atgacggtca tgcagttttg
      721 catcggcgca ggagacgaca tggattggga cgacgcacgc gtcttcctgg cgatctaccg
      781 ccagggcacg ctgcgcggcg cggccgacgc gctgcggatc gaccaggcga ccgccggacg
      841 acggctggcc gcgctggagg gagcgctcgg cgcccgcctg ttcctgcgca cgcccagggg
      901 ctacacgccg acggccgccg gcgagcaggc cttcgaggcg atggcgcgca tggaggaggg
      961 cgctcatgcg ctgctgcggc gcatgcaggg gctcgacgac aggctcgaag gcacggtctc
     1021 gctcgcctgc accgataccg cggcgacggt gttcctgatg ccggcgctgc gccgccttca
     1081 cgaccgccac ccggacatcc tcgtgcgcct ggtcgcctcc acgcgggtca gcagcctcac
     1141 gcaccgcgag gccgacctcg cggtgcgctc ggtgcgaccg accgagcccg acctcatcgt
     1201 gcgccacctg ggccggcgcc ggctggggct gtacgccgcg tccgactacc tggccgcccg
     1261 cggcagcccc gagccgggca cgggcctcgc cggccacgac accgtcatct acgcccccga
     1321 ggtctacccc ggccagcgcg agttcctgtg caccgagccg gtcggcgccg cgcgcgtggc
     1381 gctggaggtc aacaccggaa tgatgatggc cgaggccgtc gccgcgggca tcggcatcgg
     1441 cgagctcccg caccacctcg ccgcgctcta cccggggctg gtgcgcatct ggccggatcg
     1501 cgaggatccc tacgacatgt ggctcgtgat gcatgacgac ctcaaccgca gcgcgcgcgt
     1561 gcgggcggtg gccgaggcga tcgtcgaggc cttcgaggag gaggcggccg ccgccgcggt
     1621 cagtcccggg cctcgctctc gacccactcg gcgacgcgcc gcacggcgtc gcgcggcgtg
     1681 gcgtcggcgg tgatgagcca gtaggcgtag cgcgtctcca gcggcggctc gtcgggcgca
     1741 gccagcacga ggcggccgtc gtcgacgagc ggctgcagca gcgggaagcg gccgagcgcg
     1801 acgccctggc cggcgagcgc cgcctggatc acctggtcgt actggttgaa gcgcaggact
     1861 cc
//
LOCUS       DASVLN010000681         1756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_410065, whole genome shotgun sequence.
ACCESSION   DASVLN010000681 DASVLN010000000
VERSION     DASVLN010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410065"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1756)
                     /gene="sctC"
                     /locus_tag="VF453_16135"
     CDS             complement(<1..>1756)
                     /gene="sctC"
                     /locus_tag="VF453_16135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HEX7689244.1"
                     /translation="LRARGTARLAAALLAACLAAGTTASPAAPARQPFADKRFVYRAD
                     GKKLSEVLQDFAASQDLPIVVDPGVTGTVNANFNQRPQEFLDTLSRTYGVIWYFDGTT
                     LFVYPSQAMQSKVFRMRGYDRAQVRQMLGALGLGDARFPLRFDDANQTLLAYGPPRHI
                     ELVSTVLEQLERNNRDRVGASIQVVRLRFATAADRVVGNERIDGLASTLNNLFSNGAK
                     EQDPTSSLKAMGLDPVANSAALADRMRTMQSAYGFKMQPNAPATQGYGTVKREDAAKA
                     NQLDTGKPAESYDAEKPYFQAEPSTNSIIVRGLPDRMHEYESLIHQLDVQQDLVEIEA
                     SIIDVDTDDFESLGIDWNFTKDGVTRFSMSPGSPAAQPGGSVSDALATANITTLVGNA
                     GRQLLSHILLLQGKGKAHILARPKVLGTVNRAATMSDKRTASVRVAGNLDASLYSVET
                     GTTLSVTPQIIPYDDHREVHLTLQIQDGDFEGTSVDNVPIVKQTEIDTEAIVREGESL
                     LIGGISVESENTARTGVPVLSSIPLLGAAFRHDEKSKSRVERLFLLTPKIVAVGRVAA
                     TTPAPTAAPTPAPGADPAA"
BASE COUNT          237 a          568 c          661 g          290 t
ORIGIN      
        1 aggcggccgg atccgccccg ggcgccggcg tgggtgccgc ggtcggcgcc ggcgtggtgg
       61 ccgcgacgcg gccgacggcg acgatcttgg gcgtcagcag gaacaggcgc tcgacgcgcg
      121 acttcgactt ctcgtcgtga cggaacgccg ctcccaggag cgggatgctc gagagcaccg
      181 ggacgccggt gcgcgcggtg ttctccgact ccaccgagat gccgccgatc agcaggctct
      241 cgccctcgcg cacgatggcc tcggtgtcga tctcggtctg cttgacgatg ggcacgttgt
      301 ccaccgaggt gccctcgaag tcgccgtcct ggatctgcag cgtgagatgc acctcgcgat
      361 ggtcgtcgta gggaatgatc tgcggcgtca cggacagcgt ggtgcccgtc tcgaccgagt
      421 acaggctggc gtcgaggttg cccgccacgc ggaccgaggc ggtgcgcttg tccgacatcg
      481 tcgccgcgcg gttgaccgtg ccgagcacct tgggccgcgc gaggatgtgc gccttgccct
      541 tgccctgcag cagcaggatg tgcgacagca gctgcctgcc cgcgttgccc accagcgtcg
      601 tgatgttggc ggtggccagc gcgtcggaca cggagccgcc cggctgcgcc gccggcgagc
      661 ccggcgacat cgagaagcgc gtcacgccgt ccttcgtgaa gttccagtcg atgcccaggg
      721 actcgaagtc gtcggtgtcg acgtcgatga tcgaggcctc gatctcgacg agatcctgct
      781 gcacgtcgag ctggtggatg agcgactcgt actcgtgcat gcgatcgggc aggccgcgca
      841 cgatgatcga gttggtcgag ggctcggcct ggaagtacgg cttttccgcg tcgtaggact
      901 cggcgggctt gccggtgtcg agctggttgg ccttggccgc gtcctcgcgc ttgaccgtgc
      961 cgtagccctg ggtggcgggc gcgttgggct gcatcttgaa gccgtaggcc gactgcatgg
     1021 tgcgcatgcg gtcggcgagc gcggccgaat tggcgaccgg gtcgagcccc atggccttca
     1081 gcgacgaggt cggatcctgc tccttcgcgc cgttcgagaa gaggttgttc agcgtcgagg
     1141 ccaggccgtc gatgcgctcg ttgccgacga cgcgatcggc cgcggtggcg aagcgcaggc
     1201 gcaccacctg gatcgacgcg ccgacgcggt cgcggttgtt gcgctcgagc tgttcgagca
     1261 cggtgctcac gagctcgatg tggcgcggcg ggccgtaggc gagcagcgtc tggttggcat
     1321 cgtcgaagcg cagcgggaac cgggcgtcgc cgaggccgag cgcgcccagc atctggcgca
     1381 cctgcgcgcg gtcgtagccg cgcatgcgga acaccttgct ctgcatcgcc tgcgacggat
     1441 agacgaacag cgtcgtgccg tcgaagtacc agatgacgcc gtaggtgcgc gagagcgtgt
     1501 ccaggaactc ctgcgggcgc tggttgaagt tggcgttgac cgtgccggtg acgccggggt
     1561 cgacgacgat gggcaggtcc tggctcgccg cgaagtcctg caggacctcg ctcagcttct
     1621 tgccgtcggc gcgatagacg aagcgcttgt ccgcgaacgg ctggcgtgcc ggagcggccg
     1681 gcgatgccgt cgtgcccgcg gccaggcagg ccgccaggag cgccgcggcc aggcgggccg
     1741 tgccgcgcgc gcggag
//
LOCUS       DASVLN010000682         5270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_196104, whole genome shotgun sequence.
ACCESSION   DASVLN010000682 DASVLN010000000
VERSION     DASVLN010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196104"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            373..1422
                     /locus_tag="VF453_16140"
     CDS             373..1422
                     /locus_tag="VF453_16140"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7689245.1"
                     /translation="MKTSHQSPIRLLSIAAAMTAGLAGAVHAEGITMYGVADVYAEYG
                     KGDKNEVSVNSGGVSGSRLGFDASKDLGNGMKAIAKLEAGYAIDHGNSTQGGLLFGRQ
                     VYAGLSGDFGTLTAGRQYTPQFIALDANDPFDTGAGSAVSSGIISLFASRANNSVAYS
                     SNAVGGFQFSALAAAGESGTGSSTNGSLVEANFHFGSGPFGADLTLSGMKHLADGGKN
                     ATAETLGGYYDFGAFKLLGGMQFVQNLTTLADTDDNRTEFFGGINAPFGNDVLWLGAG
                     TGKFNKVSGTTASQYSIGYMHNIVKGVDVYGVATTIKNGDNTAFSDDTATGAGPAVSA
                     GKSASALQVGFRYRF"
     gene            1565..2470
                     /locus_tag="VF453_16145"
     CDS             1565..2470
                     /locus_tag="VF453_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7689246.1"
                     /translation="MALVLRLLLPAGLALASLAAHADDSATLRKIRESGVVTIGYRES
                     ELPFSYLDASQHPIGYTMDICDRVVDAVEQRLGLRDLERRYVAVTSATRIPMVANGTV
                     DMECGVTTNTVERERDVAFTVTTFVAESRLLSKRSAPVMRLEDMRGKVVTSTVGTTSL
                     THLQDLYASHGLDMTILPAKDDPDAFRFVVTDRAAAYAMDDALLRGTIARSEAPQDYV
                     VSSARLSVEPYGIMIPKGDPQFKRLADDVITGLFRSGEIWALYKRWFQSPIPPKGITL
                     DLPPFPAMQRLVAHPTDSPDPAAYP"
     gene            complement(2528..3880)
                     /locus_tag="VF453_16150"
     CDS             complement(2528..3880)
                     /locus_tag="VF453_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7689247.1"
                     /translation="MFEGFKVALIDDDPPVRRSLVQTLELAGFEVEAHGSAEAALHGL
                     APGFAGVVVTDVRLPQMDGMALLERVMQLDRSIPVILITAHGDVNLAVQAMRAGAYDF
                     LEKPFAPERLVEVTTRAMDKRALSAEVQALRQQLAARRGIESTLIGRSPAMLALQQQV
                     QRLAATAADVMIVGETGTGKELVARCLHEYSPRRERHFVAINCGGLPETLLESELFGH
                     EMGAFTTAAKKRVGKFEHADGGTLLLDEIESMPLSFQVKLLRVLQERRVERLGSNDEI
                     PIDVRVVAATKADLRELSEQGKFRSDLYYRLNVAVLHLPPLRERREDIPLLFEHFVAQ
                     ASARHGLAAEPLGEAMSKELMAHDWPGNVRELRNAAERFVLGLGDGTLLGAGPEALTL
                     AQQMDQVEKALIERALKRHQGRMNVTCEALGIARKTLYDKIAKLHIDVEAYRAGGTAD
                     "
     gene            complement(3887..>5270)
                     /locus_tag="VF453_16155"
     CDS             complement(3887..>5270)
                     /locus_tag="VF453_16155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689248.1"
                     /translation="DIADPCAQEAVHGESSQRFSPDPVTGAPEVCFARPVRRDGATVG
                     AVVVRISLQPIEAEWIDAAFRAESEKPLIVDAHGTVIMSSVPSWKQLPLAALAHPVPA
                     LDDNADLVSIPRLAAAGVEMQVVHERPLPRLGWRLLILTSAKDVWRDARIAAWSAGAI
                     TASLGMLALLVLQRRRVGMQKLATRAALQRANDELEAKVLERTLQLETSNRYLRHEVL
                     ERQHAEEVLRHAQEELVQAGKLALLGQLSAGISHELGQPLTALRALAQNGCLLLERQR
                     PLQAAENFQSILALAERMGRITSQLKSFARKTPADDRSLPFVVAIDNARQVLAQRFST
                     EEVRLVTDVPAGLAVRCDTYRLEQVLVNLMANAMDAMRTVPGDRVLTISAATAADRAV
                     VRVLDSGPGIPQELSERLFEPFFTTKAPGEGLGLGLVISSHIVREFGGSLRSVPQAAG
                     AMFEFDVARG"
BASE COUNT          745 a         1951 c         1822 g          752 t
ORIGIN      
        1 ccgcgagcag gcgcgcgcgc cccgcgcggc gcgcgcgcga gcgctccgca cgggcgcgtg
       61 cacgcgcgag cagattgcgc tcccggccgc gtcgatcgtg cggatttcgc gcccgacatc
      121 cgcgcgaatc gagcgttcgg cggccgcaga ttgcgccagt cgttcggggt cgcggacaca
      181 gacttctccc cgtgccgcga cgaccccgcc gccctcgcag acgagggccg agcgagggcc
      241 gggcgagcac gccgggctgc cggccgagag ggttcgcgag gggtggtcga cgcatgcgct
      301 gcgtcggccg cggtagtgcg tgcgcttccc ggtccggcgg catgacgaca acatcatccg
      361 agaacgacac acatgaagac ttcccaccaa tcgcccatcc gcctcctttc gatcgccgcc
      421 gcgatgaccg cgggcctggc cggtgccgtc cacgcggaag gcatcaccat gtacggcgtc
      481 gccgacgtct acgccgagta cggcaagggc gacaagaacg aggtctcggt gaactcgggc
      541 ggcgtctcgg gctcgcgcct gggcttcgac gcctccaagg acctgggcaa cggcatgaag
      601 gcgatcgcca agctcgaggc cggctacgcc atcgaccacg gcaattcgac ccagggcggg
      661 ctgctgttcg gccgccaggt ctacgccggc ctgtcgggcg acttcggcac gctgaccgcc
      721 ggccgccagt acacgccgca gttcatcgcg ctggacgcca acgacccgtt cgacaccggc
      781 gccggctcgg cggtctccag cggcatcatc agcctgttcg cgtcgcgcgc gaacaactcg
      841 gtggcgtaca gctccaacgc ggtcggcggc ttccagttct cggcgctggc cgcggcgggc
      901 gagtccggca ccggctcgag caccaacggc tcgctggtcg aggcgaactt ccacttcggc
      961 agcggcccct tcggcgccga cctcacgctg agcggcatga agcacctggc cgacggcggc
     1021 aagaacgcca ccgccgagac gctgggcggc tactacgact tcggcgcgtt caagctgctg
     1081 ggcggcatgc agttcgtcca gaacctgacc acgctggccg acaccgacga caaccgcacc
     1141 gagttcttcg gcggcatcaa cgcgccgttc ggcaacgacg tgctgtggct cggcgcgggc
     1201 accggcaagt tcaacaaggt gtcgggcacg accgcgagcc agtactcgat cggctacatg
     1261 cacaacatcg tcaagggcgt ggacgtctac ggcgtcgcga cgacgatcaa gaacggcgac
     1321 aacacggcgt tctcggacga caccgccacc ggcgccggcc cggccgtctc cgccggcaag
     1381 tcggccagcg cgctgcaggt cggcttccgc tatcgcttct gaccggcttc gaccgcgtga
     1441 tctactgagg gttggcgggg cgtcgaacgg aactcgcgcc ctgccttttt ttcggcggca
     1501 cagttcacgc cgcgggcgac tcccggcggc ggccgccgcg gcgcgcgtgc tatcgtcgtc
     1561 cgccgtggcc ctcgtcctcc gactgctcct gcccgccggc ctcgcgctcg cgagcctcgc
     1621 cgcgcacgcg gacgattccg ccacgctgcg caagatccgc gagtccggcg tcgtcacgat
     1681 cggctaccgc gagtcggagc tgccgttctc ctacctcgac gcgtcgcagc acccgatcgg
     1741 ctacacgatg gacatctgcg accgcgtcgt cgacgccgtc gagcagcgcc tgggcctgcg
     1801 cgaccttgaa cgccgctacg tcgccgtgac ctcggccacg cgcatcccga tggtggccaa
     1861 cggcaccgtg gacatggaat gcggcgtcac gaccaacacc gtcgagcgcg agcgggacgt
     1921 cgccttcacc gtcaccacgt tcgtcgccga gagccggctg ctgtccaagc gcagcgcgcc
     1981 ggtgatgcgg ctggaggaca tgcgcggcaa ggtcgtcacc tccaccgtcg gcacgaccag
     2041 cctcacgcac ctgcaggatc tctacgcgtc ccacggcctg gacatgacca tcctgccggc
     2101 caaggacgat cccgacgcgt tccgcttcgt cgtcaccgac cgcgccgcgg cctacgcgat
     2161 ggacgacgcg ctgctgcgcg gcacgatcgc gcgttcggag gcgccgcagg actacgtggt
     2221 ctccagcgcg cggctgtccg tcgagccgta cggcatcatg atcccgaagg gcgacccgca
     2281 gttcaagcgc ctggccgacg acgtgatcac cggcctgttc cgcagcggcg agatctgggc
     2341 cctgtacaag cgctggttcc agtcgccgat cccgccgaag ggcatcacgc tcgacctgcc
     2401 gccgttcccg gcgatgcagc gcctggtggc gcatccgacg gactcgccgg atcccgccgc
     2461 ctacccttga cgcggacgcc cggcccgcac gccgagatgc gccgacgcgg cggtgcggcg
     2521 ggtccgatca gtcggcggtg ccgccggcgc ggtaggcctc gacgtcgatg tgcagcttcg
     2581 cgatcttgtc gtagagcgtc ttgcgcgcga tgcccagcgc ctcgcaggtc acgttcatgc
     2641 ggccctggtg gcgcttgagc gcgcgctcga tcagcgcctt ctcgacctgg tccatctgct
     2701 gcgccagcgt gagcgcctcc ggcccggcgc ccagcagcgt gccgtcgccc aggcccagca
     2761 cgaagcgctc ggcggcgttg cgcagctcgc gcacgttgcc gggccagtcg tgggccatca
     2821 gctccttgga catcgcctcg cccagcggct ccgcggcaag gccgtgccgg gcgctcgcct
     2881 gggcgacgaa atgctcgaac agcagcggga tgtcctcgcg ccgctcgcgc agcggcggca
     2941 ggtgcagcac cgcgacgttg aggcggtagt agaggtcgct gcggaacttg ccctgctcgc
     3001 tgagctcgcg caggtcggcc ttggtcgcgg cgacgacgcg cacgtcgatc gggatctcgt
     3061 cgttggagcc caggcgctcg acgcggcgct cctgcagcac gcgcagcagc ttgacctgga
     3121 agctcagggg catgctctcg atctcgtcga gcagcagcgt gccgccgtcg gcgtgctcga
     3181 acttgccgac gcgcttcttc gcggccgtcg tgaaggcgcc catctcgtgg ccgaagagct
     3241 cgctctccag cagcgtctcg ggcagcccgc cgcagttgat cgcgacgaag tggcgctcgc
     3301 ggcgcgggct gtactcgtgc aggcagcgcg ccacgagctc cttgccggtg ccggtctcgc
     3361 ctacgatcat cacgtcggcg gcggtcgccg cgagccgctg cacctgctgc tgcagcgcga
     3421 gcatcgcggg cgatcggccg atcagcgtcg actcgatgcc gcgccgcgcc gcgagctgct
     3481 ggcgcagcgc ctgcacctcg gcgctcaggg cgcgcttgtc catcgcgcgc gtcgtcacct
     3541 cgacgaggcg ctcgggcgcg aacggcttct cgaggaagtc gtacgcgccc gcgcgcatcg
     3601 cctgcaccgc gaggttgacg tcgccgtgcg ccgtgatgag gatcaccggg atcgagcgat
     3661 ccagctgcat cacgcgctcc agcagcgcca tgccgtccat ctgcggcagc cgcacgtcgg
     3721 tgaccaccac gcccgcgaag cccggcgcca ggccgtgcag cgccgcctcg gcggagccgt
     3781 gggcctcgac ctcgaagccg gccagctcca gcgtctgcac caggctgcgg cgtaccggcg
     3841 gatcgtcgtc gatcagcgcg accttgaaac cctcgaacat ggctcgctac cctctcgcaa
     3901 catcgaattc gaacatcgcc cccgccgcct gcggcacgga gcgcagcgag ccgccgaact
     3961 cgcgcacgat gtgcgacgag atcaccagcc cgagacccag cccctcgccg ggggccttgg
     4021 tcgtgaagaa cggctcgaac aggcgctcgc tcagctcctg cgggatgccc gggccgctgt
     4081 cgaggacgcg caccaccgcg cggtcggccg cggtcgcggc gctgatcgtg agcacgcggt
     4141 cgccggggac cgtgcgcatc gcgtccatcg cgttggccat caggttgacc agcacctgct
     4201 cgagccggta ggtgtcgcag cgcacggcca ggccggcggg cacgtccgtc acgaggcgca
     4261 cctcctcggt cgagaagcgc tgggcgagca cctggcgcgc attgtcgatc gcgacgacga
     4321 agggcagcga gcggtcgtcg gccggcgtct tgcgcgcgaa ggacttcagc tgcgaggtga
     4381 tgcggcccat gcgctcggcc agcgcgagga tggactggaa gttctccgcc gcctgcagcg
     4441 gccgctggcg ctcgagcagc aggcagccgt tctgcgccag cgcccgcagc gccgtcagcg
     4501 gctggccgag ctcgtgcgag atgcccgccg acagctgccc gagcagcgcg agctttccgg
     4561 cctgcaccag ctcctcctgc gcgtggcgca gcacctcctc ggcgtgctgg cgctcgagca
     4621 cctcgtggcg caggtagcgg ttggaggtct ccagctgcag cgtgcgctcg agcaccttgg
     4681 cctcgagctc gtcgttggcg cgctgcagcg ccgcgcgcgt cgcgagcttc tgcatgccca
     4741 cgcgccggcg ctgcagcacc agcagcgcga gcatcccgag cgaggccgtg atcgccccgg
     4801 cgctccaggc cgcgatgcgg gcgtcgcgcc agacgtcctt ggcgctcgtg aggatcagca
     4861 gccgccagcc cagccgcggc agcgggcgtt cgtgcacgac ctgcatctcg acgccggcgg
     4921 cggccaggcg cgggatgctc acgaggtcgg cgttgtcgtc gagcgcgggc accggatggg
     4981 cgagcgcggc gagcggcagc tgcttccagc tcggcaccga ggacatgatg accgtgccgt
     5041 gcgcgtcgac gatcagcggc ttctcgcttt ccgcccggaa cgcggcgtcg atccactcgg
     5101 cctcgatggg ctgcaggctg atgcgcacga ccaccgcgcc gacggtggcg ccgtcgcgcc
     5161 gcacggggcg cgcgaagcag acctcgggcg cgccggtgac cgggtcgggc gagaagcgct
     5221 gcgaggactc gccgtgcacg gcctcctgcg cgcacgggtc ggcgatgtcg
//
LOCUS       DASVLN010000683         3977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_520331, whole genome shotgun sequence.
ACCESSION   DASVLN010000683 DASVLN010000000
VERSION     DASVLN010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520331"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..953)
                     /locus_tag="VF453_16160"
     CDS             complement(72..953)
                     /locus_tag="VF453_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689249.1"
                     /translation="MNAPTPAMRRLLPLLAATLLAAALSLAGPARADDDAASAPASGA
                     AALRARYDALHDQLAHNAFGRPLVLDSTQSGDRLEGRITARLDQPFAAVQQALQGTRH
                     WCSILILHLNVKMCHVQPAGLDLALGRKYDQPVDDAYKLHFDYRLDASTPDYLRATLS
                     APDGPLGTKDYRIVVEATPLDAGHTVLYMSYAYGFGLAARMAMGTYLSTIGADKVGFS
                     TEGKDGAFVGGVRGLVERNTMRYYLAIEDYVAAPGPERLDQRLNAWFDATERWPRQLH
                     ELDKSDYLAMKRAEAKR"
     gene            1109..2005
                     /gene="purU"
                     /locus_tag="VF453_16165"
     CDS             1109..2005
                     /gene="purU"
                     /locus_tag="VF453_16165"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HEX7689250.1"
                     /translation="MQATLSREREFVLKLSCRDTKGIVHAVSGLLFQAGCNIIDSQQF
                     GDVAGPGATGRFFMRVHFEAPPHLADAATLTRMMTSVGEKFEMEAQLHALAHRPRVLL
                     MVSQHGHCLNDLLFRWKSGQLDIDIPAIVSNHTTFADLAASYGIAFHHLPLAAGASAE
                     AKRAQEREVLAIVEREGVDLVVLARYMQILSPELCTALEGRAINIHHSFLPSFKGAKP
                     YYQAHDRGVKLIGATAHYVTADLDEGPIIEQDVERVDHTSTPEDFTAIGRDVECVVLA
                     RAVRWHVEHRVLRNGRKTVVFD"
     gene            2028..2477
                     /locus_tag="VF453_16170"
     CDS             2028..2477
                     /locus_tag="VF453_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7689251.1"
                     /translation="MPHQKARAAVLMASPEDAEAQFYDALRDGDLERVMAVWADDDDV
                     VCVHPGGARVAGPLAVRAAFEAIFANGTIAVHPEHVRRVHAMGCAVHSVLERIDVATA
                     EGVRTGWIVATNVYVKTAQGWRMVAHHASPGAEDERGGAGEAPSILH"
     gene            2576..3601
                     /locus_tag="VF453_16175"
     CDS             2576..3601
                     /locus_tag="VF453_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7689252.1"
                     /translation="MSAIPMPSVPMSSPVFAYRAPWWLPGGHLQTIWTSLFSRTHDGT
                     PPDFRRERWTTPDDDFVDVDFLVEPPGADRSRPMLVLFHGLEGSSTSGYAQAFACLAR
                     ELGYAYAVPHFRGCSGELNLAPRAYHSGDHEEVDWILERLRTRRGGPVLAVGISLGGN
                     MLLRWAEEHGTQAARKATAIAAVCSPLDLAAGGRAIGRGFGRHSYTRMFLGTMKPKAL
                     AKLRQHPGLFDGDRLRAARDLYEFDNAFTAPVHGFRDTEDYWSRASSKPRLADIRIPA
                     LVLNARNDPFVPADSLPTQAEVGRHVTLWQPREGGHVGFAHGALPRGMATAMPQAVFG
                     WLRDNGR"
     gene            3645..>3977
                     /locus_tag="VF453_16180"
     CDS             3645..>3977
                     /locus_tag="VF453_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEX7689253.1"
                     /translation="MDEMVRAALKKWPHVPACTGWLALDARGDWYMRDDRAQRAGPFP
                     ASKGSRIEHRALREFVERNYEATPAGEWFFQNGPQRVYVELEAAPHVWRLEAATGGAP
                     AVRSHAGAQ"
BASE COUNT          536 a         1466 c         1442 g          533 t
ORIGIN      
        1 ctgacgcggc cggcccatcg acgaacccgg cctgcgcccg cgccggcgcg ccgggttcgc
       61 cggcgcgggc gtcagcgctt cgcctcggcc cgcttcatcg cgaggtagtc ggacttgtcg
      121 agctcgtgca gctggcgcgg ccagcgctcg gtggcgtcga accaggcgtt caggcgctgg
      181 tcgagccgct cggggccggg ggcggcgacg tagtcctcga tcgcgaggta gtagcgcatc
      241 gtgttgcgct ccaccagccc gcgcacgccg ccgacgaacg cgccgtcctt gccttcggtg
      301 ctgaagccga ccttgtccgc gccgatcgtc gacaggtagg tgcccatcgc catgcgcgcg
      361 gccaggccga agccgtaggc gtagctcatg tagagcaccg tgtggccggc gtccagcggc
      421 gtcgcctcga ccacgatgcg gtagtccttc gtgcccagcg ggccgtcggg cgcggacagc
      481 gtcgcccgca ggtagtcggg cgtgctcgca tcgaggcggt agtcgaagtg cagcttgtag
      541 gcgtcgtcga ccggctggtc gtacttgcgg cccagcgcca ggtccagtcc tgccggctgc
      601 acgtggcaca tcttgacatt gaggtgcagg atcaggatcg agcaccagtg ccgcgtgccc
      661 tgcagggcct gctggacggc ggcgaagggc tggtcgaggc gcgcggtgat gcggccctcg
      721 agccggtcgc cggactgcgt ggagtccagc acgagcggcc gcccgaacgc gttgtgggcg
      781 agctggtcgt gcagggcgtc gtagcgggcg cgcagggcgg cggcgccgga agccggcgcg
      841 ctggccgcgt cgtcgtcggc gcgggccggg cccgccagcg acagcgcggc ggcaaggagg
      901 gtggcggcga gcaggggcag gagacggcgc atcgcggggg tcggtgcgtt catggtcgga
      961 tgttctcacg gcgtccgcgg tgggcgcggg ggcgcgcagg cgcaagaagc ggcggtgcgg
     1021 tccgcggcga aggcggcggc gacgtttcgc cgaggacgaa cggcataccc gtcggcaacc
     1081 cccgtgcccg tccttacaat gcgcacccat gcaagcgacc ctgtcacgcg agcgcgaatt
     1141 cgtcctcaag ctgtcgtgcc gcgacacgaa gggcatcgtc cacgccgtct ccggcctgct
     1201 gttccaggcc ggctgcaaca tcatcgactc ccagcagttc ggcgacgtcg ccggccccgg
     1261 cgcgaccggg cgcttcttca tgcgggtgca cttcgaggcg ccgccgcacc tggccgacgc
     1321 cgcgacactg acccgcatga tgaccagcgt cggcgagaag ttcgagatgg aagcgcagct
     1381 gcatgccctc gcgcaccgcc cgcgcgtgct gctgatggtg agccagcacg gccactgcct
     1441 gaacgacctg ctgttccggt ggaagagcgg ccagctcgac atcgacatcc cggcgatcgt
     1501 ctccaaccac acgaccttcg ccgacctggc cgcgagctac ggcatcgcgt tccaccacct
     1561 gccgctggcc gcgggcgcga gcgccgaggc caagcgcgcg caggagcgcg aggtgctggc
     1621 gatcgtcgag cgcgagggcg tcgacctggt cgtgctcgcg cgctacatgc agatcctctc
     1681 gcccgagctg tgcacggcgc tcgagggccg ggcgatcaac atccaccaca gcttcctgcc
     1741 cagcttcaag ggcgcgaagc cgtactacca ggcgcacgac cgcggcgtga agctgatcgg
     1801 cgcgaccgcg cactacgtga cggccgacct cgacgagggg ccgatcatcg agcaggacgt
     1861 cgagcgcgtc gaccacacct cgacgcccga ggacttcacc gccatcggcc gcgacgtcga
     1921 gtgcgtcgtc ctcgcgcgcg cagtgcgctg gcacgtggaa caccgcgtgc tgcgcaacgg
     1981 ccgcaagacc gtcgtcttcg attgagagcc cgtccggacc cgccgccatg ccgcaccaga
     2041 aagcccgcgc cgccgtcctg atggcctcgc ccgaggacgc cgaggcccag ttctacgacg
     2101 ccctgcgcga cggcgacctc gagcgcgtga tggccgtctg ggccgacgac gacgacgtgg
     2161 tgtgcgtgca cccgggcggc gcccgcgtgg ccggcccgct ggcggtgcgc gcggcgttcg
     2221 aggcgatctt cgccaacggg acgatcgcgg tgcatcccga gcacgtccgc cgcgtgcacg
     2281 cgatgggctg cgccgtccac agcgtgctgg agcgcatcga cgtcgccacc gccgagggcg
     2341 tgcgcaccgg ctggatcgtc gccaccaacg tctacgtgaa gacggcccag ggctggcgca
     2401 tggtcgctca ccacgcgagc cccggcgccg aggacgagcg cggcggcgcg ggcgaggcgc
     2461 cgtccatcct gcattgagcg cggcgggccg cgacccatcg cgcggcggat cgggctgccc
     2521 ggcctggccc ccgaattgcc ctgtcggcta ccacgccaac cgtcgccgcc gtaccatgtc
     2581 cgcgatcccg atgccctccg tgcccatgtc ctcgcccgtc ttcgcctacc gcgcgccctg
     2641 gtggctgccg ggcgggcacc tgcagacgat ctggacctcg ctgttctcgc gcacgcacga
     2701 cgggacgccc ccggacttcc gccgcgaacg ctggacgacc cccgacgacg acttcgtcga
     2761 cgtcgacttc ctcgtcgagc cgccgggtgc cgatcgttcg cggccgatgc tggtgctgtt
     2821 ccacggcctc gagggctcgt cgacgagcgg ctacgcccag gccttcgcct gcctcgcgcg
     2881 cgagctgggc tacgcctacg cggtgcccca cttccgcggc tgctcgggcg agctcaacct
     2941 cgcgccgcgc gcctaccact cgggcgacca cgaggaggtc gactggatcc tcgagcgcct
     3001 gcgcacccgc cgcggcgggc cggtgctggc ggtggggatc tcgctgggcg gcaacatgct
     3061 gctgcgctgg gccgaggagc acggcacgca ggccgcgcgc aaggcgaccg cgatcgcggc
     3121 ggtgtgctcg ccgctggacc tcgccgcggg cgggcgggcg atcggccgcg gcttcggccg
     3181 ccacagctac acgcgcatgt tcctgggcac gatgaagccc aaggcgctgg ccaagctgcg
     3241 gcagcacccc ggcctgttcg acggcgacag gctgcgcgcc gcgcgcgacc tctacgagtt
     3301 cgacaacgcc ttcacggcgc cggtgcacgg cttccgcgac accgaggact actggtcgcg
     3361 cgcctcgtcc aagcccaggc tcgccgacat ccgcatcccg gcgctggtgc tcaatgcgcg
     3421 caacgacccg ttcgtgccgg cggactcgct gcccacgcag gccgaggtcg gccgccatgt
     3481 cacgctgtgg cagccgcgcg agggcggaca cgtcggcttc gcgcacggcg cgctgccgcg
     3541 cgggatggcg acggcgatgc cgcaggcggt cttcggctgg ctgcgggaca acggccgctg
     3601 acgcgccgcc gcatcggcgc ccgggcctcg acgaaggaat ccgcatggac gagatggtgc
     3661 gcgccgcgct gaagaagtgg ccccacgtgc ccgcctgcac gggctggctc gcgctcgacg
     3721 cccgcggcga ctggtacatg cgcgacgacc gcgcccagcg tgccggcccg ttccccgcgt
     3781 ccaagggcag ccgcatcgag caccgcgcgc tgcgcgagtt cgtcgagcgc aactacgagg
     3841 cgacgcccgc gggcgagtgg ttcttccaga acgggccgca gcgggtgtac gtcgagctcg
     3901 aggcggcgcc gcacgtctgg cgcctcgagg ccgcgaccgg cggcgcgccg gccgtgcgct
     3961 cgcacgcggg cgcgcag
//
LOCUS       DASVLN010000684         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_249643, whole genome shotgun sequence.
ACCESSION   DASVLN010000684 DASVLN010000000
VERSION     DASVLN010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249643"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1596)
                     /locus_tag="VF453_16185"
     CDS             complement(43..1596)
                     /locus_tag="VF453_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7689254.1"
                     /translation="MASHYSLRQKLWAPLVVCWLALLALTLFNAFEARTKAYDARRQA
                     LSDVIQMADALIAGIKADVQAGKLTDDAGKAEALARVGALRYSNGAGYVTIVGTDSVV
                     LNNPASPKINGKNMADFQDAKGNYLYKAIAAAGVAGSGYIEYWWPRPGAKEPSAKLSF
                     VKRSPSWGWDLVAGDYIDDIQQRFVDSLVKSVLALVVVGALLTAIATAVTRSVLRSIG
                     GEPALAAAMARRIAEGDVSRHGPAVAAAEGSVLHAVERMRQQLDTLVSRIHGSAEAIT
                     RSVREIASGSLDLSSRTESQASSLEETAASMEQLTATVRQNAENAGKAGELAESASTV
                     AEQGGSAVDQVVQTMEAITGSSRRIADIIGVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVASEVRTLAQRSASAAKEIKTLIQDSVEKVDAGSALVDRAGVTMRDVVANVRNV
                     TGIVSAIAMASREQRSGIEQVNQSVVAMDQVTQQNAALVEQSTAATQMLQAEVDRLVE
                     TVRVFKVSA"
BASE COUNT          239 a          687 c          680 g          282 t
ORIGIN      
        1 gcccgggagt cccgcgcggc ggatgacgcc gggccgcggc cctcaggcgc tcaccttgaa
       61 gacgcgcacc gtctccacca gccggtcgac ctcggcctgc agcatttggg tcgcggcggt
      121 cgactgctcg accagcgcgg cgttctgctg ggtcacctgg tccatcgcga cgaccgactg
      181 gttgacctgc tcgatgccgc tgcgctgctc gcggctggcc atcgcgatcg cggagacgat
      241 gcccgtcacg ttgcgcacgt tggcgacgac gtcgcgcatc gtgacgcccg cgcggtcgac
      301 cagcgcgctg ccggcgtcga ccttctcgac cgaatcctgg atcagcgtct tgatctcctt
      361 ggccgccgag gcgctgcgct gggccagcgt gcgcacctcg gaggccacga cggcgaagcc
      421 gcggccctgc tcgcccgcgc gcgcggcctc gactgccgcg ttcagcgcca ggatgttggt
      481 ctggaaggcg atgccgtcga tcacgccgat gatgtcggcg atcctgcgcg agctgccggt
      541 gatcgcctcc atggtctgga cgacctggtc gaccgcgctg ccgccctgct ccgcgaccgt
      601 ggacgcgctc tcggcgagct cgccggcctt gcccgcgttc tcggcgttct ggcgcacggt
      661 ggcggtgagc tgctccatcg aggcggcggt ctcctcgagc gagctggcct gcgactcggt
      721 gcggctcgag aggtcgaggc tgccggaggc gatctcgcgc accgagcgcg tgatcgcctc
      781 ggccgagccg tggatgcgcg agaccagcgt gtcgagctgc tggcgcatgc gctccaccgc
      841 gtgcagcacg ctgccctcgg cggccgcgac ggccggcccg tgacgcgaga cgtcgccctc
      901 ggcgatgcgg cgcgccatcg cggcggccag ggccggctcg ccgccgatcg agcgcagcac
      961 gctgcgcgtc accgcggtgg cgatggccgt cagcagcgcg cccaccacga cgagcgcgag
     1021 caccgacttc acgagcgagt cgacgaagcg ctgctggatg tcgtcgatgt agtcgcccgc
     1081 gacgaggtcc cagccccacg agggcgagcg cttgacgaag gacagcttgg cgctcggctc
     1141 cttggcgccg ggacgcggcc accagtactc gatgtagccc gaacccgcga cgcccgcggc
     1201 ggcgatcgcc ttgtagaggt agttgccctt ggcgtcctgg aagtcggcca tgttcttgcc
     1261 gttgatcttc gggctcgccg ggttgttcag caccacggag tcggtgccga cgatcgtgac
     1321 gtagcccgcc ccgttcgagt agcgcagcgc cccgacgcgc gcgagcgcct ccgccttgcc
     1381 ggcgtcgtcg gtcagcttgc cggcctggac gtcggccttg atgccggcga tcagcgcgtc
     1441 ggccatctgg atcacgtcgg acagcgcctg gcggcgcgcg tcgtaggcct tggtgcgcgc
     1501 ctcgaacgcg ttgaagagcg tcagtgccag cagcgccagc cagcagacga cgaggggagc
     1561 ccagagcttc tggcggagcg agtagtggga ggccatggga agggtcgggt gagcggtcac
     1621 tggaggacgc gcgacggccg gtcgcgcacg gaagatcagc gcttgcgatc ttggccgacc
     1681 cgcgcggcga accatcgcgc gagttggcag gcgatgcggg cccaaatcgg gcgcttcgtg
     1741 gcccggacgg gccgccgcac aatcggggcc tccgggctac ccgccgaccg ccgtgacctt
     1801 ccccccgacc gacccgcgcg ccctgctgcg ccgcctgttc gacgccgccg tcgccgccgc
     1861 ccagccggcg ctgtgcatgc cgccgcac
//
LOCUS       DASVLN010000685         4747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_34228, whole genome shotgun sequence.
ACCESSION   DASVLN010000685 DASVLN010000000
VERSION     DASVLN010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34228"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VF453_16190"
     CDS             <1..537
                     /locus_tag="VF453_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689255.1"
                     /translation="ARPRGSRQHRAMHIRRFLAAALLGLSATFAVAAPAAFVADPAVE
                     ATAEADAEQAVAMAKRQFGIALDWSDRSIDDVEHALAGLHTSYALASPKPSDEQLMPL
                     AKAFGAYFGEVYRRNHGAAWGRVTMNGETYPGIHTAAGVDVWPAGRVMNRITDGPDND
                     VAIYYRRLAEPKRADDAE"
     gene            complement(676..1857)
                     /locus_tag="VF453_16195"
     CDS             complement(676..1857)
                     /locus_tag="VF453_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7689256.1"
                     /translation="MNLKRRFPGGRIGRSARVAALAVAASAMGVLAGCDRTPDVVKIG
                     VAQPITGPLGALGQDMLNGAKMAVDEINANGGVNVHGKMVHLAIVSQDDKGSNDAGDA
                     AARKLVDEDVEVAIADLNSGVSIHAAPIYAAANIPQLAISTKPEFTHLGFPTTLRLVA
                     SDDLQSKALGSYAAQLPGVEHYAVVDDSTPYGKGLADQAAKVLGDLGKKVEVRKSLDD
                     KTTDFASLVGEFKAAKIDLLVTTLADFQVDALMDEAAKQGLTNLTIVGGDTIKTDRLP
                     QKTIPIRAVYATSSIVEPKEFPGGKVFLEKFRRLYKSDPVYGAHYAYDAVYLVADAMS
                     RNASVDRGDLLKTLKSFDGNCPMTNSMRFGADGEQRYAAVAVYQLRNGAWDPLMRSDR
                     W"
     gene            complement(2065..2706)
                     /locus_tag="VF453_16200"
     CDS             complement(2065..2706)
                     /locus_tag="VF453_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEX7689257.1"
                     /translation="MAWTRDEMAARAARELQDGFYVNLGIGLPTLVANHVPAGMEVWL
                     QSENGLLGIGPFPTEDEVDADMINAGKQTVTTLPGSSIFSSADSFGMIRGGKINLAIL
                     GAMQVSEKGDLANWMIPGKMVKGMGGAMDLVAGVGRVVVLMEHVATKKDGTTDLKIIP
                     SCTLPLTGVGVVDRIITDLCVLDVGPQGLKLVELAPGVTLDEVQQRTGAPIAH"
     gene            complement(2748..3449)
                     /locus_tag="VF453_16205"
     CDS             complement(2748..3449)
                     /locus_tag="VF453_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX7689258.1"
                     /translation="MNKVYPSAAAALQGIVKDGQMLAVGGFGLCGIPEALIDALRDTG
                     AKDLTVISNNAGVDGFGLGKLLATRQIAKMISSYVGENKEFERQYLSGELQLEFTPQG
                     TLAEKLRAGGAGIPAFFTKTGVGTIVAEGKETREFDGQKYVMELSLKPDVSLVKAWKA
                     DKSGNLMFRRTARNFNPNVAQAGRITVAEVEEIVETGSFDPDQVHLPGIFVHRLVLNA
                     HPEKRIEQRTTRKAA"
     gene            3575..3982
                     /locus_tag="VF453_16210"
     CDS             3575..3982
                     /locus_tag="VF453_16210"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7689259.1"
                     /translation="MDVPALQTLHAEIFAPWVRELGLVVRATEADAVTLALPVRPAHV
                     HGGGVVCGQTLMAAADTALVLAISHVLGGFRPQTTVQLQASFLRPVGGDVPELIVTAR
                     VLKAGRSLVFGEVSLALPDGRLAARATSTTALL"
     gene            3998..4618
                     /locus_tag="VF453_16215"
     CDS             3998..4618
                     /locus_tag="VF453_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS and helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7689260.1"
                     /translation="MSPIRHALLAEAADPETLRARTAELRAAFDYRTAFDLAPIGLVL
                     SRNRLMIDCNRAVLAMFRAEREQLVGQSFRVLYPTQGEYERTGERIVASLDVDGRYAD
                     ERVMRRVDGELFWCHVSGRALDSGDPHAAGIWSFDDLSSQRSLKLDFTAREREIAALL
                     IEGLTSKLIGKRLAISPRTVDVYRARLMRKVGAATTAELVHKLLAG"
BASE COUNT          670 a         1678 c         1651 g          748 t
ORIGIN      
        1 gcgcggccgc gaggctcgcg ccagcatcgc gcgatgcaca tccgccgctt cctcgccgcc
       61 gccctgctcg gcctcagcgc gaccttcgcg gtcgccgcgc cggcggcgtt cgtcgccgac
      121 cccgcagtcg aggcgaccgc cgaggcggat gccgagcagg cggtggcgat ggccaagcgg
      181 cagttcggca tcgcgctcga ctggagcgac cgcagcatcg acgacgtcga gcatgcgctc
      241 gcgggcctgc acacgagcta cgcgctcgcc tcgcccaagc cgtccgacga gcagctgatg
      301 ccgctggcca aggcgttcgg cgcctacttc ggcgaggtct atcgccgcaa ccacggcgcc
      361 gcctggggcc gggtgacgat gaacggcgag acgtacccgg gcatccacac cgccgcgggc
      421 gtggacgtct ggccggccgg ccgcgtgatg aaccgcatca cggacgggcc ggacaacgac
      481 gtcgcgatct actaccggcg gctggccgag ccgaagcgcg cagacgacgc ggaatgagcg
      541 gagcctgcgc gttgccggcg ctgccgctcg agtgcgccga acgagacgca gggaagcggc
      601 agatccggct ttgccggcct gcacgcgcac ggcccctcgg gggcagggag cgccagcgac
      661 cgtggggccg tcaattcacc agcgatcgga gcgcatcagc ggatcccacg ccccgttgcg
      721 cagctggtag accgcgaccg cggcatagcg ctgctcgccg tccgcgccga agcgcatcga
      781 gttggtcatc gggcagttgc cgtcgaagct cttgagcgtc ttcagcaggt cgccgcggtc
      841 gacgctcgcg ttgcgcgaca tcgcgtcggc caccaggtag acggcgtcgt acgcgtagtg
      901 cgcgccgtag accggatccg acttgtagag gcggcggaac ttctcgagga agaccttgcc
      961 gcccgggaac tccttgggct cgacgatcga ggacgtcgca tagaccgcgc ggatcgggat
     1021 cgtcttctgc ggcagcctgt cggtcttgat cgtgtcgccg ccgacgatgg tgaggttggt
     1081 caggccctgc ttggcggcct cgtccatcag cgcatcgacc tggaagtcgg ccagcgtggt
     1141 gaccagcagg tcgatcttgg cggccttgaa ctcgccgacc agcgacgcga agtcggtggt
     1201 cttgtcgtcc agcgacttgc gcacctcgac cttcttgccg aggtcgccca gcaccttggc
     1261 ggcctggtcg gccaggccct tgccgtaggg cgtcgaatcg tcgacgacgg cgtagtgctc
     1321 gacgccgggc agctgcgcgg cgtaggagcc gagcgccttg gactgcaggt cgtcgctcgc
     1381 gaccaggcgc agcgtcgtgg ggaagcccag gtgcgtgaac tccggcttgg tcgagatcgc
     1441 cagctggggg atgttggcgg ccgcgtagat cggcgccgcg tggatgctga cgccggagtt
     1501 gaggtcggcg atcgcgacct cgacgtcctc gtcgaccagc ttgcgggccg cggcgtcgcc
     1561 ggcgtcgttg ctgcccttgt cgtcctgcga gacgatggcc aagtgcacca tcttgccgtg
     1621 cacgttcacg ccgccgttgg cgttgatctc gtcgacggcc atcttcgcgc cgttgagcat
     1681 gtcctggccg agcgcgccga gcggcccggt gatcggctgc gcgacgccga tcttgacgac
     1741 gtcgggcgtg cgatcgcaac ccgcgaggac gcccatggcc gaagcggcga ccgcgagggc
     1801 cgccacgcga gccgaccggc cgatccggcc accaggaaag cgacgcttca gattcatgcg
     1861 actgaccctc gaaaatcgtt ggtccggccc gcacggaggg gccggtgtgg cgcgtgccgc
     1921 catgccgcgg cgcgctgggc cattatggaa gcgcggtctc gaggatcaga cgatttcggg
     1981 gccccgcagg ccccgaagtt gacgaatatt tgacacctgg cggggcaaag cgacgcccca
     2041 aggcgacgct gcccgcacgg cggttcagtg cgcgatcggc gcgccggtgc gctgctgcac
     2101 ctcgtccagc gtgacgccgg gtgcgagctc cacgagcttc aggccctgcg gcccgacgtc
     2161 cagaacgcac aggtccgtga tgatgcggtc gacgacgccc acgccggtca gcggcagcgt
     2221 gcacgacggg atgatcttca ggtcggtcgt gccgtccttc ttcgtcgcga cgtgctccat
     2281 cagcaccacg acgcggccga cgccggccac gaggtccatc gcgccgccca tgcccttgac
     2341 catcttgccc gggatcatcc agttggcgag gtcacccttc tcgctgacct gcatcgcgcc
     2401 caggatcgcg aggttgatct tgccgccgcg gatcatgccg aagctgtccg cgctggagaa
     2461 gatgctcgag ccgggcagcg tcgtcaccgt ctgcttgccg gcgttgatca tgtcggcgtc
     2521 gacctcgtcc tcggtcggga acgggccgat gcccagcagg ccgttctcgc tctgcagcca
     2581 cacctccatg cccgcgggca cgtgattggc gaccagcgtg ggcaggccga tgcccaggtt
     2641 cacgtagaag ccgtcctgca gctcgcgcgc ggcgcgcgcg gccatctcgt ctcgggtcca
     2701 agccatttgt cgttgctccg gagtcttgtt cgggtcgtgt cgtcgggtca ggcggccttg
     2761 cgcgtcgtgc gctgctcgat gcgcttctcg ggatgcgcgt tgagcacgag ccggtgcacg
     2821 aagatccccg gcaggtgcac ctggtcggga tcgaagctgc ccgtctcgac gatctcctcg
     2881 acctcggcga cggtgatgcg ccccgcctgg gcgacgttcg ggttgaagtt gcgcgcggtg
     2941 cggcggaaca tcaggttgcc cgacttgtcg gccttccagg ccttcacgag cgagacgtcc
     3001 ggcttcagcg agagctccat gacgtacttc tggccgtcga actcgcgcgt ctccttgccc
     3061 tcggccacga tggtgccgac gcccgtcttg gtgaagaacg ccgggatgcc cgcgccgccg
     3121 gcgcgcagct tctcggcgag cgtgccctgc ggcgtgaact cgagctgcag ctcgcccgag
     3181 aggtactggc gctcgaactc cttgttctcg ccgacgtagc tcgagatcat cttcgcgatc
     3241 tgccgcgtcg cgaggagctt gcccagaccg aagccgtcga cgcccgcatt gttcgagatg
     3301 accgtcaggt ccttcgcgcc ggtgtcgcgc agcgcgtcga tcagggcctc ggggatgccg
     3361 cacaggccaa agccgccgac ggccagcatc tggccgtcct tcacgatgcc ttgcagcgcc
     3421 gcggcggcgc tcgggtagac cttgttcatg cgcgcgtgtc tccagtggga agcgacaaag
     3481 ttggcggccg cgaggatcgt cgcgccctcg tccggcacaa gcgaagaagc gtactactta
     3541 tccgattacg tagccattac gtagaatcaa ttacatggac gtccccgccc tccagaccct
     3601 ccacgccgag atcttcgccc cctgggtgcg cgagctcggg ctcgtcgtgc gcgccacgga
     3661 ggccgacgcc gtcacgctgg cgctgccggt gcggcccgct cacgtgcatg gcggcggggt
     3721 ggtctgcggc cagacgctga tggccgcggc cgacaccgcg ctcgtgctgg cgatcagcca
     3781 cgtcctgggc ggcttccggc cgcagaccac cgtgcagttg caggcgagct tcctgcgccc
     3841 ggtcggcggc gacgtgccgg agctcatcgt caccgcgcgc gtgctgaagg ccggccgcag
     3901 cctcgtgttc ggcgaggtga gcctcgcgct gcccgacggc cggctcgccg cccgggccac
     3961 cagcaccacc gcgctgctct gagccgcggc cacggacatg tccccgatcc gccacgccct
     4021 cctggccgaa gccgccgatc ccgagacgct gcgggcacgc accgccgagc tgcgcgccgc
     4081 gttcgactac cgcacggcgt tcgacctcgc gccgatcggc ctcgtgctct cgcgcaaccg
     4141 gctgatgatc gactgcaacc gcgcggtgct ggcgatgttc cgcgccgagc gcgagcagct
     4201 cgtcggccag tctttccgag tgctctaccc gacgcagggc gagtacgagc gcaccggcga
     4261 acgcatcgtc gcgagcctgg acgtggacgg ccgctacgcc gacgagcgcg tgatgcggcg
     4321 cgtcgacggc gagctgttct ggtgccacgt gagcggccgt gcgctggatt ccggcgaccc
     4381 acacgccgcc ggcatctgga gcttcgacga cctttcgtcg cagcgctcgc tcaagctcga
     4441 cttcacggcg cgcgagcgcg agatcgcggc cctgctgatc gagggcctca cgagcaagct
     4501 gatcggcaag cgcctcgcga tcagcccgcg caccgtggac gtctaccgcg cgcggctgat
     4561 gcgcaaggtc ggggcggcga cgaccgcgga gctcgtgcac aagctgctgg cgggttgacc
     4621 cgccgcgcgc ggcgcggccg cgggatgctc aggcgccggt gacccgcagc gcctccacgc
     4681 cctggccgta cggacgcagc agcgcgtgca gcgcgaacgc cgcgccgccg actgcggtgg
     4741 ccagcgt
//
LOCUS       DASVLN010000686         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_304883, whole genome shotgun sequence.
ACCESSION   DASVLN010000686 DASVLN010000000
VERSION     DASVLN010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304883"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VF453_16220"
     CDS             <1..244
                     /locus_tag="VF453_16220"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7689261.1"
                     /translation="LANLLFALNPARVIVGGGITARGPAFLEELRAATARRLRPDFHR
                     MCDIQLASAGNHAGLLGAARLWLDEHGAAIGARASA"
     gene            263..2023
                     /locus_tag="VF453_16225"
     CDS             263..2023
                     /locus_tag="VF453_16225"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEX7689262.1"
                     /translation="MQILLNHVGYRPASAKRAIVQHGAPFEPCEFSVVNAATRAVVLR
                     GRLAPLAPVPGWKGRHFAAADFDELAAPGRYFLVLDGTWPPVHSQDFEIGETLFGSQM
                     LSDVVHYFKSQRCTGLFDDADRGAPLLDTREPRDVHGGWYDASGDCSKYLSHLSYANF
                     MNPQQTPLVAWTLMEGRARIPAQSKWLDERIVDEALHGADFLVRMQAPEGFFYTTVFD
                     RWSKDERQREICSYATQKGHKSADYQAGFRQGGGLAIAALARASGLPRDGAFPREDYL
                     ERAILGFEVLERHNAEFLDDHRENLIDHYCALLAATELHARTGDERWALAAIRRVDAM
                     KALQRVDGGYWMDEARTRSFFHASDAGLPHVALMRFLEVLRALDPQAPTMVVRSMVSR
                     GLMHELRMTRDGSPNPFGYPRQFVAMPGREGGVRFFVPHDNESGYWWQGENARLGSLA
                     AAARMAARLDIAEDDVPAGELARRFADPLDAYADRILDWILGANPFDACMLQGWGHHP
                     PRYEPGFWNAAGGVCNGVTAGLEDEDDIDFRKPEDTEPMHSWRWTEQWIPHAAWLFAA
                     LCWGLTTPQPPHPAFVAEGR"
BASE COUNT          305 a          823 c          748 g          299 t
ORIGIN      
        1 cctcgccaac ctgctgttcg cgctcaaccc ggcgcgcgtg atcgtcggcg gcggcatcac
       61 cgcgcgcggg ccggccttcc tcgaggagct gcgcgccgcg accgcgcgcc gcctgcggcc
      121 cgacttccac cgcatgtgcg acatccagct cgcgagcgcc ggcaaccacg ccggactgct
      181 gggcgccgcg cggctgtggc tcgacgaaca cggcgcggcc atcggcgcgc gcgcatccgc
      241 ctgatcgacg aggagccggg ccatgcagat cctcctgaac cacgtcggct accgcccggc
      301 cagcgccaag cgcgcgatcg tgcagcacgg cgcgcctttc gagccctgcg agttctccgt
      361 cgtgaacgcc gccacgcgcg cggtcgtgct gcgcggccgg ctcgcgccgc tcgcgcccgt
      421 gccgggctgg aagggccgcc acttcgcggc cgcggacttc gacgaactcg ccgcgcccgg
      481 ccgctacttc ctcgtgctgg acggcacctg gccgccggtc cattcgcagg acttcgagat
      541 cggcgagacg ctgttcggca gccagatgct gtcggacgtc gtgcactact tcaagtcgca
      601 gcgctgcacc gggctgttcg acgacgccga ccggggcgcc ccgctgctgg acacgcgcga
      661 gccgcgcgac gtgcacggcg gctggtacga cgcgtcgggc gactgctcga agtacctctc
      721 gcacctgtcg tacgccaact tcatgaaccc gcagcagacg ccgctcgtcg cgtggacgct
      781 gatggaaggc cgcgcgcgca tcccggcgca gtcgaagtgg ctcgacgagc gcatcgtcga
      841 cgaggcgctg cacggcgccg acttcctcgt gcgcatgcag gcgcccgagg gcttcttcta
      901 cacgacggtg ttcgacaggt ggagcaagga cgagcgccag cgcgagatct gctcgtacgc
      961 gacgcagaag ggccacaagt ccgccgacta ccaggccggc tttcgccagg gcggcggcct
     1021 cgcgatcgcc gcgctcgcgc gcgcctcggg cctgccgcgc gacggcgcgt tcccgcgcga
     1081 ggactacctc gagcgagcga tcctcggctt cgaggtgctc gaacggcaca acgcggagtt
     1141 cctcgacgac caccgcgaga acctgatcga ccactactgc gcgctgctgg ccgccaccga
     1201 gctccatgcg cgcaccggcg acgaacggtg ggcgctcgcc gcgatccgcc gcgtcgacgc
     1261 gatgaaggcg ctgcagcggg tcgacggcgg ctactggatg gacgaggcgc gcacgcgcag
     1321 cttcttccac gcgagcgatg ccgggctgcc gcacgtcgcg ctcatgcgct tcctcgaggt
     1381 gctgcgcgcg ctcgacccgc aggcgccgac gatggtcgtg cgctcgatgg tctcgcgcgg
     1441 cctgatgcac gagctgcgca tgacgcgcga tggaagcccc aacccgttcg ggtatccgcg
     1501 ccagttcgtc gccatgccgg gccgcgaggg cggggtgcgc ttcttcgtcc cgcacgacaa
     1561 cgagagcggc tactggtggc agggcgagaa cgcccgcctc ggctcgctcg ccgcggccgc
     1621 gcgcatggcc gcgcgcctgg acatcgccga ggacgacgtg ccggccggcg agctcgcccg
     1681 ccgcttcgcc gatccgctgg acgcctacgc cgaccgcatc ctcgactgga tcctcggcgc
     1741 caatccattc gacgcctgca tgctgcaggg ctggggccac cacccgccgc gctacgagcc
     1801 cgggttctgg aacgcggccg gcggcgtgtg caacggcgtc accgccggcc tcgaggacga
     1861 ggacgacatc gatttccgca agcccgaaga caccgagccc atgcactcgt ggcgttggac
     1921 cgagcagtgg atcccccatg ccgcgtggct gttcgccgcg ctctgctggg gactcaccac
     1981 gccgcagccg ccgcaccccg ctttcgtggc agaaggccgc tgaccccgca gcgtcgccgg
     2041 agccgaactt gaggccgcct tcctggagga agggggcggc cggaggcctt gtcatgcgat
     2101 tccagacgac ccacctcgcg tccgcggtgc tgatgctcac ggggacgatc cagtgcgccc
     2161 acgcgcagtc ggccg
//
LOCUS       DASVLN010000687         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_520530, whole genome shotgun sequence.
ACCESSION   DASVLN010000687 DASVLN010000000
VERSION     DASVLN010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520530"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VF453_16230"
     CDS             <1..558
                     /locus_tag="VF453_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7689263.1"
                     /translation="LRNSVAWGPRTAVWSSLGNVTGLFGLSAAAMLGLGVLLQSSAVL
                     FGLVKGVGAGYLFYVGARQLLGKGVSLVPPAGGLPAAPPARRRLFGEAVLTASTNPKP
                     ILFFTALFPQFLDPRAALLPQFLVLTGVFMALSFASLFGYALVAARARSVLMRPRFSL
                     WVGRGVGAIFVGFGTALLALRRQAG"
     gene            complement(579..1397)
                     /locus_tag="VF453_16235"
     CDS             complement(579..1397)
                     /locus_tag="VF453_16235"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7689264.1"
                     /translation="MDYHAIREAIDSVDYKLFALGKTQITVFTLVKLAISALLLYLVA
                     GRLSRWTLRRLLGRTKMEQGQQLAIIALVHYGVLVFGAMLILQNAGINLTAFAMVGSA
                     LGVGVGFGLQNIISNFISGLIVLLERPIEIGDRIELAGVEGVVREIGARRTTIITPDH
                     IAILVPNQKFITDNVTNYLYFDRSVRLRVPLAIGPGQDIRHVESVLLAAASHADVHAD
                     PKPEVVITGMSASAVSLELWVWYDGQSFARVDVANRVYHAAFDALAAAGIKMGS"
     gene            complement(1441..1656)
                     /locus_tag="VF453_16240"
     CDS             complement(1441..1656)
                     /locus_tag="VF453_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689265.1"
                     /translation="MHRPDPSSSTLAAHAAAFVLAALATTSMLLATSHIARAERACAQ
                     RAFAARLARADGPAAVRPHAAAPRRLA"
     gene            complement(1689..1937)
                     /locus_tag="VF453_16245"
     CDS             complement(1689..1937)
                     /locus_tag="VF453_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689266.1"
                     /translation="MNTTCSPAASRAFANAPAPTGLRRWLALALEAHRQHLESRDAVE
                     LELLARTRLARGDAPARTPESLRDAYRLFGGGDPLRLL"
     gene            2050..3012
                     /locus_tag="VF453_16250"
     CDS             2050..3012
                     /locus_tag="VF453_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7689267.1"
                     /translation="MSRIALNTLPAFVAAAQLQNLRAAAEALHLTHSAVSQQIASLEE
                     RLGFPLFDRRGRRIVLNAAGEALLGSVAPALQRIQEGVQAATVTARGAGQTLRLTILP
                     SFAERWLLPRLARWRERHPDILLDLDPSNELVDLVRDGFHAGLRTGTGPWPGLVVQRL
                     YDGPTNFIAVAEPNRARRLAAGPREAMAREPLLGDRGIWARWFAAAGINAQPVPVAAF
                     GEPGLMLQAVEQGLGIAVVRELFAADALQDGRLVKLFDVSFVHERVSPHSLVYPEGLK
                     DWPPVLALRDWLRDEFESSRRELASAQVPAAFLEPRAKDDGAAQ"
     gene            3097..>3528
                     /locus_tag="VF453_16255"
     CDS             3097..>3528
                     /locus_tag="VF453_16255"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEX7689268.1"
                     /translation="MPPPLPPTRLPTYFISHGGGPWPWMKAQLGGAYDRLEASLARMP
                     AEVGAAPRAILMVSAHWEAPEFTVQTHPNPPMLYDYGGFPEFTYHVRYPAPGAPALAP
                     RVRELLAAAGIASAEDAQRGFDHGLFSPMAVAWPQADVPIAQ"
BASE COUNT          439 a         1231 c         1330 g          528 t
ORIGIN      
        1 ctgcgcaaca gcgtggcctg ggggccgcgg acggccgtgt ggtcgtcgct gggcaacgtg
       61 acggggctgt tcgggctgtc ggcggcggcg atgctggggc tgggcgtgct gctgcagtcg
      121 tcggcggtgc tgttcgggct cgtgaagggg gtcggtgcgg gctacctgtt ctacgtcggc
      181 gcgcgccagc tgctgggcaa gggcgtctcg ctggtgccgc cggccggcgg gctgccggcc
      241 gcgccgcccg cgcggcggcg gctgttcggc gaggccgtgc tgacggcgag caccaacccc
      301 aagcccatcc tgttcttcac cgcgctgttc ccgcagttcc tcgacccgcg ggccgccctg
      361 ctgccgcagt tcctcgtgct gaccggcgtg ttcatggcgc tgtcgttcgc gtcgctgttc
      421 ggctatgcgc tggtcgccgc gcgcgcgcgc tcggtgctga tgcggccgcg cttctcgctg
      481 tgggtcggac gcggggtcgg cgcgatcttc gtcggcttcg gcacggcgct gctcgcgctg
      541 cggcggcagg cgggctgaag gatccggcgc gccggcgatc aggatcccat cttgatgccc
      601 gcggccgcga gcgcgtcgaa ggcggcgtgg tagacgcggt tggccacgtc gacgcgcgcg
      661 aaggactggc cgtcgtacca gacccagagc tcgaggctca ccgcgctcgc cgacatgccg
      721 gtgatgacga cctccggctt cgggtcggca tgcacgtcgg cgtggctggc ggctgcgagc
      781 agcacgctct ccacgtggcg gatgtcctgc cccggcccga tcgcgagcgg cacgcgcagc
      841 cgcaccgagc ggtcgaagta gaggtagttc gtcacgttgt cggtgatgaa cttctggttc
      901 ggcacgagga tggcgatgtg gtcgggggtg atgatggtcg tgcggcgggc gccgatctcc
      961 cgcacgacgc cctcgacgcc cgcgagctcg atgcggtcgc cgatctcgat cgggcgctcc
     1021 agcagcacga tcaggccgct gatgaagttg ctgatgatgt tctgcaggcc gaagccgacg
     1081 cccacgccga gcgcgctgcc gaccatcgcg aacgcggtga ggttgatgcc cgcgttctgc
     1141 aggatcagca tcgcgccgaa gacgagcacg ccgtagtgca cgagcgcgat gatcgcgagc
     1201 tgctggcctt gctccatctt cgtgcgcccg agcagccggc gcagcgtcca gcggctcagg
     1261 cgcccggcga cgaggtagag cagcagggcg gagatcgcca gcttcacgag cgtgaagacc
     1321 gtgatctgcg tcttgcccag ggcgaacagc ttgtagtcga cggagtcgat cgcttccctg
     1381 atggcgtggt agtccaagac cggcattcct cgaggctggg atcgaggcgc ggggcaattt
     1441 tcatgcgaga cggcgcggcg ccgcggcatg cgggcggacg gcggcgggcc cgtcggcgcg
     1501 ggccaggcgg gcggcgaagg cccgctgcgc gcacgcacgt tcggcgcgcg cgatgtgcga
     1561 ggtggccagc agcatcgagg tcgtcgcgag ggcggcgagc acgaacgcgg cggcgtgcgc
     1621 cgccagcgtg gacgaggagg ggtcgggacg gtgcatggcg gtctcctgtt cggttcgggt
     1681 gggcggcgtc agagcagccg cagcggatcg ccgccgccga acaggcggta ggcgtcgcgc
     1741 agcgactcgg gcgtgcgcgc aggtgcgtcg ccgcgcgcga ggcgcgtgcg tgcgagcagc
     1801 tccagctcga cggcgtcgcg cgattccagg tgctggcgat gggcctccag cgccagcgcg
     1861 agccagcggc gcaggccggt cggggccggg gcgttcgcga aggcacggga cgcggcaggg
     1921 gagcaggtcg tgttcatggc gttctccttg aggcttgcgt tcgaggggtg tgctggtaag
     1981 aatagccacc tgcttcgcgc tgaggaatcg attcttcaag gcgaaattgg ttagaagtgc
     2041 ttaccagaga tgtcccggat cgccctcaac acgctgcccg cgttcgtcgc cgccgcccag
     2101 ttgcagaacc tgcgcgcggc cgccgaggcg ctgcacctca cgcacagcgc ggtcagccag
     2161 cagatcgcct cgctcgagga gcgcctgggt ttcccgctgt tcgaccgccg cggccggcgc
     2221 atcgtcctca atgccgcggg cgaggcgctg ctgggcagcg tcgcgccggc actgcaacgc
     2281 atccaggagg gcgtgcaggc ggcgaccgtg accgcgcgcg gcgccggcca gacgctgcgg
     2341 ctgacgatcc tgccgtcctt cgcggagcgc tggctgctgc cgcgactggc gcgctggcgc
     2401 gagcgccatc cggacatcct gctggacctc gatccgtcca acgagctggt cgatctcgtg
     2461 cgcgacggct tccacgccgg gctgcgcacg ggcaccggcc cctggcccgg cctcgtcgtc
     2521 cagcgcctgt acgacggccc gaccaacttc atcgcggtgg cggagccgaa tcgcgcgcgc
     2581 cgcctggccg ccggcccgcg cgaggccatg gcgcgcgagc cgctgctggg cgaccgcggc
     2641 atctgggcgc gctggttcgc ggcagccggc atcaacgccc agccggtgcc ggtggccgcg
     2701 ttcggcgagc cgggcctgat gctgcaggcc gtggagcagg gcctgggcat cgcggtcgtg
     2761 cgtgagctgt tcgccgccga cgcgctgcag gacgggcgcc tcgtcaagct gttcgacgtc
     2821 agcttcgtgc acgagcgcgt ctcgccgcac agcctcgtct atcccgaggg cctgaaggac
     2881 tggccgcccg tgctcgcgct gcgcgactgg ctgcgcgacg agttcgagag ttcgcgccgc
     2941 gagctcgcgt ccgcgcaggt gcccgcggcc ttcctcgagc cacgcgcgaa ggacgacggc
     3001 gccgcgcaat gaccggccgc gggcacgggt tcgcgtcgcg gcctaagctt cgcgcgccgc
     3061 cgtccccggc accgcaccga caccatcgag accgacatgc cgccgcccct gcctcccacg
     3121 cgcctgccca cgtacttcat ctcccacggc ggcggcccct ggccctggat gaaggcgcag
     3181 ctggggggcg cctacgaccg gctggaggcc tcgctcgcgc gcatgcccgc cgaggtcggc
     3241 gccgcgccgc gggcgatcct gatggtgtcc gcgcactggg aagcgcccga gttcaccgtg
     3301 cagacgcacc cgaacccgcc gatgctctac gactacggcg gcttccccga attcacctac
     3361 cacgtgcgct acccggcccc gggcgcgccc gcgctcgcgc cgcgcgtgcg cgagctgctg
     3421 gccgccgccg gcatcgcatc ggcggaggat gcgcaacgcg gcttcgatca cggcctgttc
     3481 tcgccgatgg cggtggcgtg gccgcaggcc gacgtgccga tcgcgcag
//
LOCUS       DASVLN010000688         4283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_250022, whole genome shotgun sequence.
ACCESSION   DASVLN010000688 DASVLN010000000
VERSION     DASVLN010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250022"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VF453_16260"
     CDS             <1..556
                     /locus_tag="VF453_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-aminobenzoate-CoA ligase"
                     /protein_id="HEX7689269.1"
                     /translation="ACVLDADGRALPRGQVGRLAVRGPTGCRYLDDERQAQYVQFGWN
                     LTGDAYLQDEDGFFRYQARTDDMIISAGYNIAAPEVETALLEHPAVAECAVIGVPDEE
                     RGQIVKAFVVLRAGHEPGLGMVRTLQEFVKATVAPYKYPRAIEFRAELPRTATGKLQR
                     FRLRPESTVALPPSVSDASRGSDA"
     gene            553..957
                     /locus_tag="VF453_16265"
     CDS             553..957
                     /locus_tag="VF453_16265"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7689270.1"
                     /translation="MKTVLQPEGWPRPRGYANGVAAQGRQVFVAGMVGWNAAGEFESD
                     DLAAQVRQALLNVRAVLEEAGARPEHMVRMTWYVVSRAEYLARGREIGAAYREVLGAH
                     YDVAMSAVEVSALMEARARVEIEVTAVVPQAL"
     gene            1112..2473
                     /locus_tag="VF453_16270"
     CDS             1112..2473
                     /locus_tag="VF453_16270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7689271.1"
                     /translation="MRRFLSSTAVDAPPYIAVPASRALHTGFVKKQLSMTIRTATAPQ
                     LPFSALAAAILVALAASASAAPHHPKPRPKAHASARKEAAAEPAMTKDANGLSVPVLD
                     NKAWVLMDFNTGQILGSSNADEPLPPASLTKMMTSYLVEQGLKSGKLKPNEPVTMSLS
                     AWCRGGKEGDSCMFVPLNAQASLMDMLRGVVIDSGNDASKALAEHIAGSESAFATLMN
                     AEAAKLGMTHTHFENSTGLPDPSHKASARDLAVLAQAIIRNSGDYYPIYAEHEFTYNG
                     IKQGNRNALLYTDPTVDGLKTGHTEEAGFCLTASAKRNGMRLISVIMNASSAQARADQ
                     TRVLFNWGYGTFEEATPAQAGATLATAKVRYGVVPTVAAGVAQAWTLVVPKGQGAAVQ
                     TQVSLKPDLEAPIAKGAVIGKVVASANGKQLGEAPVIAMAAVERAGFFQRIGQRISGL
                     FSK"
     gene            complement(2657..>4283)
                     /locus_tag="VF453_16275"
     CDS             complement(2657..>4283)
                     /locus_tag="VF453_16275"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7689272.1"
                     /translation="MPDAAASAAAPAAPAADDTALRAALGQATVAGNFRTTYMGYDAD
                     KRFVQVPPQKLPDGYDPTARPWYKAAAAADKPVVTEPYKDASFGDLTVTFALPVKADG
                     KLVGVLGADKSLAEVVKTVAAIKPSENSYAFILDKDGRILVHPNTDYALKPLAEAVPG
                     LAAVATAPVNQIVETNDGTRDLWVRTEAIPGTDWRLAVALDKSAAMAGLGTLVGSIAF
                     AALGVVAVVALLLATVLRAWFAPLRKVRDAMVAVAGGGGDLTSRIAAPSDDEVGQIAH
                     AFNDFTDSLRGIIREVQQASAEVKTAANEIATGNADLSARTESQASSIQQSASSIAQL
                     HQTTLANADSAREANRIAQEAATLAAEGGTKVGEVIETMGGITSSSKRIGEIIGVIDS
                     IAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRTLAQRSGEAARDIRQLIQKSTEDV
                     EAGATRVDDAGRTISQVVESVRSVSALMARIVEATQQQTGGIGEVNNAVTQIDQMTSQ
                     NAALVEEASAAASSLRDQSARLVDAVGRFRTAH"
BASE COUNT          618 a         1535 c         1538 g          592 t
ORIGIN      
        1 ggcctgcgtg ctcgacgccg acggccgcgc gctgccgcgc ggccaggtcg gccgcctcgc
       61 ggtacgcggg ccgacgggct gccgctacct cgacgacgag cgccaggcgc aatacgtgca
      121 gttcggctgg aacctcaccg gcgacgccta cctgcaggac gaggacggct tcttccgcta
      181 ccaggcgcgc accgacgaca tgatcatctc ggccggctac aacatcgccg cgccggaggt
      241 cgagaccgcg ctgctcgaac atcccgccgt cgccgaatgc gcggtgatcg gcgtgcccga
      301 cgaggagcgt ggccagatcg tcaaggcctt cgtggtgctg cgggccggcc acgagcccgg
      361 cctcggcatg gtgcggacgc tgcaggagtt cgtgaaggcg accgtcgcgc cgtacaaata
      421 cccgcgcgcg atcgagttcc gcgccgagct gccgcgcacc gcgaccggca agctgcagcg
      481 cttccgcctc cgcccggagt ccaccgtggc cctgccccca tccgtatccg acgcctcgcg
      541 agggagtgac gcatgaagac cgtcctgcaa ccggaaggct ggccgcggcc tcgcggctac
      601 gcgaacggcg tcgccgcgca gggccgccag gtcttcgtcg ccggcatggt gggctggaac
      661 gccgccggcg agttcgagag cgacgacctc gccgcccagg tgcggcaggc gctgctcaac
      721 gtgcgcgcgg tgctcgagga agcgggcgcg cggcccgagc acatggtgcg gatgacgtgg
      781 tacgtcgtgt cgcgcgccga gtacctcgcc cggggccgcg agatcggcgc ggcctatcgc
      841 gaggtgctgg gcgcgcacta cgacgtcgcg atgagcgcgg tcgaggtcag cgcgttgatg
      901 gaggcgcggg cgcgggtgga gatcgaggtc acggccgtcg tgccgcaggc gctctgaagc
      961 ggcgtgggcc gacgccggtc gacgaagccg gctatcgcgc gaacgcgggc gcttcggccg
     1021 ccgatcgtcg cgatcggggg cggccggaca gaacttcccg gcgccagccg ggtcggtgga
     1081 agtgcgcgtc cgtcacaatc cacgctgttc catgcgccgc ttcctgtcga gcacggccgt
     1141 ggacgcccca ccgtacatcg cggtgcccgc ctcgcgggcg ctgcataccg gcttcgtgaa
     1201 gaagcagctc tcgatgacca ttcgcaccgc cactgcaccc cagcttccct tctccgccct
     1261 cgccgccgcg atcctcgtcg ccctggcggc cagcgcctcc gccgcaccgc atcatccgaa
     1321 gcccaggccc aaggcccatg cctccgcccg caaggaggcc gcggccgagc ccgcgatgac
     1381 gaaggacgcg aacgggctgt cggtgcccgt gctcgacaac aaggcctggg tcctgatgga
     1441 cttcaacacg ggtcagatcc tcgggtcgtc caacgcggac gagccgctgc ccccggcgtc
     1501 gctgaccaag atgatgacca gctacctggt ggagcagggg ctcaagagcg gcaagctcaa
     1561 gccgaacgag ccggtcacca tgagcctgag cgcctggtgc cgcggcggca aggagggcga
     1621 cagctgcatg ttcgtgccgc tcaatgcgca agcgtcgctg atggacatgc tgcgcggcgt
     1681 cgtgatcgac agcggcaacg atgcctcgaa ggccctggcg gagcacatcg cgggctcgga
     1741 gtccgccttc gcgaccctga tgaacgccga ggcggcgaag ctgggcatga ctcacaccca
     1801 cttcgagaac tcgaccggcc tgcccgaccc cagccacaag gcctcggcgc gcgacctggc
     1861 ggtgctcgca caggcgatca tccgcaacag cggcgactac tacccgatct acgcggagca
     1921 cgagttcacc tacaacggca tcaagcaggg caaccgcaac gcgctgctgt acaccgatcc
     1981 gaccgtcgac gggctcaaga ccggccacac cgaggaagcc ggcttctgcc tgacggcgtc
     2041 ggccaagcgc aatggcatgc gcctgatctc ggtcatcatg aacgcgagca gcgcgcaggc
     2101 gcgcgccgac cagacgcgcg tgctgttcaa ctggggctac ggcaccttcg aggaagcgac
     2161 gccggcccag gcaggggcga cgctggccac cgccaaggtg cgctacggcg tcgttcccac
     2221 ggtcgccgcc ggcgtcgcgc aggcctggac gctcgtcgtg ccgaagggcc aaggcgccgc
     2281 ggtccagacc caggtgtccc tgaagccgga cctcgaggcg ccgatcgcca agggcgccgt
     2341 gatcggcaag gtcgtcgcct cggccaacgg caagcagctc ggcgaagcgc cggtcatcgc
     2401 gatggccgcc gtggagcgcg ccggcttctt ccagcgcatc ggccaacgca tctccggcct
     2461 gttctcgaag tgaggacggc cgtcaccggc gcggtggccc cggagcggcc gagggcgaca
     2521 ccccgtcgac gcgccgcggg tccgcgcacg gccggcaccg ccgccaagga cgcggccgtc
     2581 cgccagttcg aagactgacg gacggccggg gcgcggaaaa gggaaccgag cggcgaggag
     2641 gccccgccgc tgcgtctcag tgcgcggtgc ggaagcggcc gacggcgtcg acgaggcgtg
     2701 ccgactggtc ccgcagcgac gaggccgcgg cggacgcttc ctcgaccagc gccgcgttct
     2761 gcgaggtcat ctggtcgatc tgcgtgaccg cgttgttgac ctcgccgatg ccgcccgtct
     2821 gctgctgcgt ggcctcgacg atgcgggcca tcagcgcgct caccgagcgc accgactcga
     2881 cgacctgcga gatcgtgcgc ccggcgtcgt cgacgcgggt ggcgccggcc tcgacgtcct
     2941 cggtcgactt ctggatcaac tggcggatgt cgcgcgcggc ctcgccgctg cgctgcgcca
     3001 gcgtgcgcac ctcacccgcc accaccgcga agccgcggcc ctgctcgccg gcgcgcgcgg
     3061 cttcgaccgc ggcgttcagc gccaggatgt tggtctggaa ggcgatcgag tcgatgacgc
     3121 cgatgatctc gccgatgcgc ttgctcgacg aggtgatgcc gcccatggtc tcgatgacct
     3181 cgccgacctt ggtgccgcct tcggccgcga gcgtggcggc ttcctgggcg atgcggttgg
     3241 cctcgcgcgc cgagtcggcg ttggcgagcg tcgtctggtg cagctgcgcg atcgagctgg
     3301 ccgactgctg gatcgagctc gcctggctct cggtgcgcgc ggacaggtcg gcgttgccgg
     3361 tcgcgatctc gttggccgcg gtcttcacct cggcgctggc ctgctgcacc tcgcggatga
     3421 tgccgcgcag cgagtcggtg aagtcgttga acgcgtgcgc gatctggccg acttcgtcgt
     3481 cgctgggagc cgcgatgcgg gaggtcaggt cgccgccacc gccggccacg gcgaccatcg
     3541 cgtcgcgcac cttgcgaagg ggcgcgaacc aggcgcgcag caccgtcgcc agcagcagcg
     3601 cgacgacggc caccacgccc agcgcggcga acgcgatcga gccgaccagc gtgcccaggc
     3661 ccgccatcgc ggcggacttg tcgagcgcga ccgccaggcg ccagtcggtg cccgggatcg
     3721 cctcggtgcg cacccacagg tcgcgggtgc cgtcgttggt ctcgacgatc tggttgaccg
     3781 gcgcggtcgc gacggcagcc agccccggca ccgcctcggc cagcggcttg agggcgtagt
     3841 cggtgttcgg atggaccagg atgcggccgt ccttgtcgag gatgaacgcg tacgagttct
     3901 cgctcggctt gatggcggcg acggtcttga cgacctcggc cagcgacttg tcggcaccga
     3961 gcacgcccac cagcttgccg tcggccttga cgggcagcgc gaaggtcacg gtcaggtcgc
     4021 cgaacgatgc gtccttgtac ggctcggtca cgacgggctt gtccgcggcc gcggcggcct
     4081 tgtaccaggg gcgcgccgtc gggtcgtagc cgtccggcag cttctgcggc ggcacctgga
     4141 cgaagcgctt gtcggcgtcg tagcccatgt aggtcgtgcg gaagttgccg gcgaccgtcg
     4201 cctggccgag cgccgcacgc agcgccgtgt cgtccgcggc gggcgcagcc ggggcggcgg
     4261 cggaggccgc ggcgtcgggc atg
//
LOCUS       DASVLN010000689         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_89372, whole genome shotgun sequence.
ACCESSION   DASVLN010000689 DASVLN010000000
VERSION     DASVLN010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89372"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VF453_16280"
     CDS             <1..750
                     /locus_tag="VF453_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase"
                     /protein_id="HEX7689273.1"
                     /translation="AAGMCERRTPSGVALRGYVRNDAMAGRLRALEVVWHKALAPGYG
                     WIFPLPGGRFNIGAGIAYEHEPRPGGRAKLKDLNLRDIFAAFCDHYEPARELMRGGTL
                     EGELKGAPLRCSLVGARFARAGLLVTGEAAGSTYSFTGEGIGKAYQTGLLAADALLQG
                     RADGHVPAGADAAVRARYEAGLAALKPRFDLYERANRVNKHPWLADFLIWRARHNARL
                     LRRMAGVLEETSNPGNLVSARGLLKVMFPGR"
     gene            821..1615
                     /locus_tag="VF453_16285"
     CDS             821..1615
                     /locus_tag="VF453_16285"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX7689274.1"
                     /translation="MCQLLGMNANAPTDAMFSFTGLATRADEHKDGFGIAFFEDRGLR
                     LFVDQHSARNSPVAELVKGYPIRSDNVIAHIRKATQGRIALENTHPFMRELWGRYWVF
                     AHNGDLKDFRPRLHGHFRPAGDTDSEHAFCWLMQELAKAHAGVPAIDELSCTLAELLP
                     RLHRHGTFNMLLSNGQALWAHCSTRLHWVAREHPFGAPVLADKDLMVDFSAHTTPDDR
                     VAIVATEPLTRNEAWQAMAPGELVAFVQGVPAAPARPAACPERARA"
BASE COUNT          224 a          635 c          574 g          211 t
ORIGIN      
        1 gccgccggca tgtgcgagcg ccgcacgccc agcggcgtgg cgctgcgcgg ctatgtccgc
       61 aacgacgcga tggccggccg gctgcgcgcg ctggaggtcg tctggcacaa ggcgctcgcg
      121 ccgggctacg gctggatctt cccgctgccc ggcggccgct tcaacatcgg cgcgggcatc
      181 gcctacgagc acgagccgcg ccccgggggc cgggccaagc tgaaggacct gaacctgcgc
      241 gacatcttcg cggccttctg cgaccactac gagcccgcgc gcgagctgat gcgcggcggc
      301 acgctggagg gcgagctcaa gggggcgccg ctgcgctgct cgctggtggg tgcgcgcttc
      361 gcgcgggccg ggctgctcgt caccggcgag gccgcgggca gcacgtactc gttcaccggc
      421 gagggcatcg gcaaggccta ccagaccggg ctgctcgcgg cggacgcgct gctgcagggc
      481 cgcgcggacg ggcacgtccc ggccggcgcc gacgccgccg tgcgcgcgcg ctacgaggcc
      541 ggcctggccg cgctcaagcc gcgcttcgac ctctacgagc gcgccaaccg cgtcaacaag
      601 cacccctggc tcgcggactt cctgatctgg cgcgcccgcc acaacgcgcg cctgctgcgc
      661 cgcatggccg gcgtgctcga ggagaccagc aaccccggca acctcgtgag cgcgcgcggg
      721 ctgctgaagg tgatgttccc gggccgctga cgccgcggcg catgacgtcc tgcgcgacgc
      781 gcatgtcgcg cccgaccgcc ggacgcgtat gcttggcgcc atgtgtcaac tcctgggcat
      841 gaacgccaac gcgccgaccg acgcgatgtt cagcttcacc ggcctcgcga cccgggccga
      901 cgagcacaag gacggcttcg gcatcgcctt cttcgaggat cgcggcctgc ggctgttcgt
      961 ggaccagcac agcgcgcgca actcgccggt ggccgagctc gtcaagggct acccgatccg
     1021 cagcgacaac gtcatcgcgc acatccgcaa ggcgacgcag ggccgcatcg cgctcgagaa
     1081 cacgcatccg ttcatgcgcg agctctgggg ccgctactgg gtcttcgccc acaacggcga
     1141 cctcaaggac ttccgcccgc gcctgcacgg ccacttccgt ccggccggcg acaccgacag
     1201 cgagcacgcc ttctgctggc tgatgcagga gctcgccaag gcccacgcgg gggtgccggc
     1261 gatcgacgag ctctcctgca cgctcgccga gctgctgccg cgcctgcatc gccacggcac
     1321 cttcaacatg ctgctgtcca acggccaggc gctgtgggcg cactgctcga cgcgcctgca
     1381 ctgggtcgcg cgcgagcacc cgttcggcgc gccggtgctc gccgacaagg acctgatggt
     1441 cgacttctcc gcccacacga cgcccgacga ccgcgtcgcc atcgtcgcca cggagccgct
     1501 cacccgcaac gaggcgtggc aggcgatggc cccgggcgag ctggtcgcct tcgtccaggg
     1561 cgtgcccgcc gcgccggcgc gcccggcggc ctgccccgag cgggcgcgcg cctgacacgg
     1621 caggcgagga ctcgcacggc gctt
//
LOCUS       DASVLN010000690         4304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_196619, whole genome shotgun sequence.
ACCESSION   DASVLN010000690 DASVLN010000000
VERSION     DASVLN010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196619"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VF453_16290"
     CDS             <1..997
                     /locus_tag="VF453_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013094370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7689275.1"
                     /translation="TASRPAPPWNGPRTGPHAQPGKRIAIVDEDLRNGGILGVTDGLV
                     EASTAAGWQSRVFDCGGTPEGRRKMLADALDSRPDGLVLVGGDARSLLPDLQPFAQRG
                     IPVVGWHVAARPGPVSGTPVAMNVSTDPLEVARVTALAAVAESDGHAGVVVFTDDGFE
                     LPRVKADAMAAVVRACAGCRLLEVRDVAISRSKDAMPAVTREVLARYGSRWTHALAIN
                     DIYFDYAAPVLTQAGLASAAVAMLSAGDGSESAFLRIRSGVFQTGTVAEPLNLHGWQL
                     VDELNRLFAGQGVSGYVFPVHLVTRDNIARDGGDRLLYDPANGYRDVYRRIWQRP"
     gene            994..>4304
                     /locus_tag="VF453_16295"
     CDS             994..>4304
                     /locus_tag="VF453_16295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689276.1"
                     /translation="MKLARPQSLSAQFALGLSCLGALLAVLGATTLYALASSTEAIRE
                     LAERRLARLQDAQDLGQLTAGIERAALGLARIDRVDALRASSAALVGQLQAFDGLVDR
                     LAANAASDDVDVLALHRASQHFRNTVNIETQVRETMLAEPAGAAASASAASLARLDDD
                     LRQQADALSAAAGRQSAYFTRDYRDAVQRLAATTDRARGWVLVELGLGVLLAWGVVRE
                     FLGRRIVTRLGLVSQALQRGDEDDAQAGVPVRGGDEIAGMARAVERFLEDRRRRRAAE
                     DALQRLNADLEDRVAERTAELSAALDGRTREIAERRRAEDAARASEHFLDGIVENIPD
                     MIFVKDAATLRFVRINRAGEELLGVPREQLVGKTVHELFPTHEADFFTLKDRVVLEDR
                     RLVDIPEEWVQTGDGPRLVHTKKIPVLDAAGTPRFLLGISHDITERKRADDELRRHRE
                     HLEDMIRERTAALVVAKEQADAANRAKSDFLANMSHEIRTPMNAIVGMSYLALQSGLD
                     ARQSGYVHKVHAAARSLLGIINDILDFSKIEAGKLDIEHVAFDLGDLVDDLASLVGMS
                     AEDKGLELLFALPPELPARLVGDPTRLRQVLLNLGYNAIKFTERGEVVVSVSVLGRDE
                     RSASLRFEVRDTGIGMTPEQVQALFQPFAQADASTSRRYGGTGLGLAISRQLVRLMGG
                     EIEVDSAVGRGSTFRFTLRLGLGADAAATAAPVRGAQEGLRGRRVLIVDDNASAREVL
                     GGMTAAMEMRTDVACGGEEGLRRADEALARGEPYELVLLDWKMPGIDGIECARRLARR
                     APPDGPPPTILMLTAFGREEARRRLADEGVDVHALLVKPVTPSTLLEACCAALGVAPA
                     RTTRTAKRSEALLADQARLQGARVLLVEDNAINREIALTILDRAGIVVSVACDGREAL
                     ELLERERFDGVLMDCQMPVMDGYEATRALRRQPRWRTLPVIAMTANAMVGDRDKVLAA
                     GMDDHVAKPIDVEELFAAMARWIRPAAPAGQAPADERPIPPPVPSQDGAAVDVRAGIA
                     AMMGDEALYRRLLRMFLDRETDFPERFRRARAESQAKAAMRLAHDLKSVAGSLGAEPL
                     RRAATRLEQACVDAD"
BASE COUNT          573 a         1599 c         1589 g          543 t
ORIGIN      
        1 gacggcctcg cgccccgcgc cgccgtggaa cggcccccgc accgggccgc acgcccagcc
       61 cggcaagcgc atcgcgatcg tcgacgagga cctgcgcaac ggcggcatcc tcggcgtcac
      121 ggacggactc gtcgaggcga gcaccgcggc cggctggcaa tcgcgcgtgt tcgattgcgg
      181 cggcacgccc gaggggcgcc gcaagatgct ggccgacgcg ctggacagcc gcccggacgg
      241 cctcgtcctc gtcggcggcg acgcccgcag cctcctgccc gacctgcagc cgttcgcgca
      301 gcgcggcatc ccggtcgtcg gctggcatgt cgccgcgcgc cccgggcccg tgtccggcac
      361 gccggtcgcg atgaacgtct cgaccgatcc cctggaggtc gcgcgcgtca cggcgctcgc
      421 ggcggtcgcc gaatccgacg gccatgcggg cgtggtggtg ttcaccgacg acgggttcga
      481 gctgccgcgc gtcaaggccg acgcgatggc cgcggtcgtc cgggcctgcg ccggctgccg
      541 gctcctcgag gtgcgcgacg tcgccatctc gcgcagcaag gacgcgatgc cggccgtcac
      601 ccgcgaggtg ctggcccgct acggctcgcg ctggacgcac gcgctggcga tcaacgacat
      661 ctacttcgac tacgccgccc cggtgctgac gcaggccggg ctggccagcg ccgcggtggc
      721 gatgctgtcg gccggcgacg gcagcgagtc cgcgttcctg cgcatccgca gcggcgtctt
      781 ccagaccggc acggtcgccg agccgctgaa cctgcacggg tggcagctgg tcgacgagct
      841 caaccgcctg ttcgcggggc agggcgtgtc gggctacgtc ttcccggtgc acctggtgac
      901 gcgcgacaac atcgcgcgcg acggcggcga tcgcctgctg tacgaccccg ccaacggcta
      961 ccgcgacgtc taccgccgca tctggcaacg gccatgaagc tcgcccgtcc gcaatcgctg
     1021 tccgcgcagt tcgcgctcgg cctgtcctgc ctgggcgcgc tgctggccgt gctcggcgcg
     1081 acgacgctgt acgcgctcgc ctcgtcgacc gaggcgatcc gcgagctggc ggagcgccgg
     1141 ctcgcgcgcc tgcaggacgc ccaggacctc ggccagctga ccgccggcat cgagcgcgcc
     1201 gccctcgggc tcgcgcgcat cgaccgcgtc gacgccctgc gcgcgtccag cgccgccctc
     1261 gtcgggcagc tgcaggcttt cgacggcctc gtcgaccgcc tcgcggccaa tgcggcgagc
     1321 gacgacgtcg acgtcctggc gctgcaccgc gcgagccagc atttccgcaa cacggtcaac
     1381 atcgagaccc aggtgcgcga gacgatgctt gccgagccgg cgggcgcggc cgcctccgca
     1441 tccgcggcct ccctggcgcg gctggacgac gacctgcgcc agcaggccga cgcgctgtcg
     1501 gctgccgcgg gccggcagtc ggcctacttc acgcgcgact accgcgacgc cgtgcagcgc
     1561 ctggccgcca cgaccgatcg cgcgcgcggc tgggtgctgg tggaactcgg gctgggcgtg
     1621 ctgctcgcgt ggggcgtcgt gcgcgagttc ctcggccgcc gcatcgtcac gcgcctgggc
     1681 ctggtgagcc aggcgctcca gcgcggcgac gaggacgacg cccaggccgg cgtgccggtg
     1741 cgcggcggcg acgagatcgc cggcatggcg cgcgccgtgg agcgcttcct ggaggatcgc
     1801 cgccgccgcc gggccgcgga ggacgccctg cagcgcctga acgccgacct ggaggatcgc
     1861 gtcgccgagc gcaccgcgga gctgagcgcc gcgctggacg gccgcacgcg cgagatcgcc
     1921 gagcgccgcc gcgccgagga cgccgcgcgc gccagcgagc acttcctgga cggcatcgtc
     1981 gagaacatcc ccgacatgat cttcgtcaag gacgccgcga cgctgcgctt cgtgcgcatc
     2041 aaccgggcgg gcgaggagct gctgggcgtg ccgcgcgaac agctggtcgg caagaccgtg
     2101 cacgagctgt tcccgacgca cgaggccgac ttcttcacgc tcaaggatcg cgtggtgctg
     2161 gaggatcggc gcctcgtcga cattcccgag gagtgggtgc agaccggcga cgggccgcgc
     2221 ctggtgcaca cgaagaagat tcccgtgctc gacgccgcgg gcacgccgcg gttcctgctc
     2281 gggatctcgc acgacatcac cgagcgcaag cgcgccgacg acgagctgcg ccgccaccgc
     2341 gagcacctgg aggacatgat ccgcgagcgc acggcggcgc tggtcgtcgc caaggaacag
     2401 gccgacgccg ccaaccgcgc caagagcgac ttcctggcca acatgagcca cgagatccgc
     2461 acgccgatga acgcgatcgt cggcatgtcc tacctggcgc tgcagagcgg gctggacgca
     2521 cgccagagcg gctacgtgca caaggtccac gcggcggcgc ggtcgctgct gggcatcatc
     2581 aacgacatcc tcgacttctc caagatcgag gccggcaagc tcgacatcga gcacgtcgcc
     2641 ttcgacctcg gcgacctcgt cgacgacctc gccagcctgg tcggcatgag cgccgaggac
     2701 aagggcctcg agctgctgtt cgcgttgcct cccgagctgc ccgcgcggct cgtcggcgat
     2761 cccacccggc tgcggcaggt gctgctcaac ctcggctaca acgccatcaa gttcacggag
     2821 cgcggcgagg tcgtggtctc ggtgagcgtg ctcgggcgcg acgagcgctc ggcgtccctg
     2881 cgcttcgagg tgcgcgacac cggcatcggc atgacgcccg agcaggtgca ggcgctgttc
     2941 cagccgttcg cgcaggcgga cgcgtcgacc agccggcgct acggcggcac cggcctcggg
     3001 ctcgcgatca gccgccagct ggtgcggctg atgggcggcg agatcgaggt cgacagcgcc
     3061 gtcggccgcg gcagcacctt ccgcttcacg ctgcggctgg ggctcggcgc cgatgccgcg
     3121 gcgacggccg cgccggtgcg gggcgcgcag gaaggcctgc gtggccggcg ggtgctgatc
     3181 gtcgacgaca acgcgagcgc gcgcgaggtg ctggggggca tgaccgccgc gatggagatg
     3241 cgtaccgacg tcgcctgcgg cggcgaggag gggctgcggc gcgccgacga ggcgctcgcc
     3301 cgcggcgagc cctacgagct ggtgctgctc gattggaaga tgcccggcat cgacggcatc
     3361 gagtgcgcgc gccggcttgc ccgccgtgcg ccgccggacg gcccgccgcc gacgatcctg
     3421 atgctgaccg ccttcgggcg cgaggaggcg cgccggcgcc tggccgacga aggcgtcgat
     3481 gtccatgcgc tgctcgtcaa gccggtcacg ccctccacgc tgctcgaggc ctgctgcgcg
     3541 gcgctcggcg tcgcccccgc gcggacgacg cgcacggcca agcgcagcga ggcgctgctc
     3601 gccgaccagg cgcggctgca gggcgcgcgc gtgctgctgg tcgaggacaa cgcgatcaac
     3661 cgcgagatcg cgctgacgat cctcgaccgc gcgggcatcg tggtgagcgt cgcctgcgac
     3721 ggccgcgagg cgctggagct gctggagcgc gagcgcttcg acggcgtgct gatggactgc
     3781 cagatgcccg tcatggatgg ctacgaggcg acccgtgcgc tgcgccggca gccgcgctgg
     3841 cgaacgctgc cggtgatcgc gatgaccgcc aacgcgatgg tgggcgaccg cgacaaggtg
     3901 ctcgccgccg gcatggacga ccacgtggcc aagccgatcg acgtcgagga gctgttcgcc
     3961 gcgatggcgc gctggatccg gcccgcggcg cccgcggggc aggcgccggc ggacgagcgg
     4021 ccgatcccgc ccccggtccc gagccaggac ggcgccgcag tcgacgtgcg cgccggcatc
     4081 gcggcgatga tgggcgacga ggcgctgtac cggcgcctgc tgcgcatgtt cctcgatcgc
     4141 gagaccgact ttcccgagcg cttccgccgt gcgcgcgccg agagccaggc caaggcggcg
     4201 atgcgcctcg cgcacgacct caagagcgtg gccggctcgc tcggggccga gccgctgcgt
     4261 cgcgccgcca cgcggctcga gcaggcctgc gtcgatgcgg acga
//
LOCUS       DASVLN010000691        10600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_410539, whole genome shotgun sequence.
ACCESSION   DASVLN010000691 DASVLN010000000
VERSION     DASVLN010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410539"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..824
                     /locus_tag="VF453_16300"
     CDS             48..824
                     /locus_tag="VF453_16300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7689277.1"
                     /translation="MTLRIAIVDDEAPARAKLRRYLAAAPDVHVVGEAASGREALGLL
                     VEHRPDVVFLDVRMPDMDGFAVLQSLGEPLAIEVVFVTAHGDHALQAFEVHAFDYLLK
                     PVSPERFDRLLRRLRERLVARADAAGRVDRLLAELPATAHPAESHAEHLLVEADGRGE
                     LLPVDGIVQVEADRNYLVVHATDGRRFRLRGTVERIQARLDPARFARVNRSTLVRLAA
                     VREVRPWPDGEYRLLLEDGGRVTWTKRYLEQAAARLLLRL"
     gene            941..2785
                     /locus_tag="VF453_16305"
     CDS             941..2785
                     /locus_tag="VF453_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7689278.1"
                     /translation="MDRRDLLKAALAAPFATGFAPACAADEASATAAGAAAGWSRVRP
                     GDAAWPGPDAWDALSRRVGGRLAKLASPFAGCGGADAGTACGDAARAAANPFWIGDQP
                     ALTQAGGWADAWLSQPSAWGVAAANAADVAAAVDFAREHRLRLVVKGGGHGYQGVSSA
                     PDSLLVWTRHMNAVTLHDAFVARGCDAAPQPAVSLGAGAMWIDAYDAVTTRAGRYVQG
                     GGCTTVGVAGLVQSGGFGSFSKRFGTAAAGLLEAEVVTADGQVRVVNACSHPDLFWAL
                     KGGGGGSFGVVTRVTLRTHELPETFGAVRGTIRAKDDTAFRALLARVVDFHADVLHNP
                     HWGEQIGLRTDNSLHFALAFQGLTQAQAEEAWRPLCEWLSAYADALEVVEPIGIHALP
                     PRRYWDAAFWRRVAPGVMVCDDRPGAPPHHAEWAGDRKQAGMFLHGYESAWLPARLLA
                     HEERPRLVDALFAATRHFEVGLHFNKGLAGAADAEIAAARDTATNPAVLDAFALAIIA
                     TAGSSAWPGVAPQAPDLAEARRHRAAIGLAMTELKKAAPGAGSYVSESDYFLRDWPTA
                     FWGANHARLAAVKQRYDPQGLFFVRHGVGSEAWSDDGFTRIAASQPQA"
     gene            complement(2871..2946)
                     /locus_tag="VF453_16310"
     tRNA            complement(2871..2946)
                     /locus_tag="VF453_16310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2911..2913),aa:Lys,seq:ctt)
     gene            complement(3090..3165)
                     /locus_tag="VF453_16315"
     tRNA            complement(3090..3165)
                     /locus_tag="VF453_16315"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3130..3132),aa:Lys,seq:ttt)
     gene            complement(3156..4094)
                     /locus_tag="VF453_16320"
     CDS             complement(3156..4094)
                     /locus_tag="VF453_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEX7689279.1"
                     /translation="MSSTAAASSATGPVVDDFPLEADLERRFGGLKRLYGAAGYARVR
                     AARVAVVGLGGVGSWAVEALARSGVAQLVLVDLDHVAESNVNRQVQAVGATIGMSKAE
                     ALARRIADIHPGCRVHGVEEFADETNWPGLLPVEVDAVIDACDQSRAKLMLADWARRT
                     CMPFVTCGAAGGKQRAEKVEIDDLHATTHDPLLAGLRQRLRARHGAPRSGRMGVRCVF
                     SRESVAPPAVDDEDAACDVDPSAVRRDNSLNCHGYGSTVTVTATFGLAAAGEVIAQLI
                     AAKAVDPVRRDAQAIDGLARQRAGMLTFMALPETGC"
     gene            complement(4111..4926)
                     /gene="ybgF"
                     /locus_tag="VF453_16325"
     CDS             complement(4111..4926)
                     /gene="ybgF"
                     /locus_tag="VF453_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX7689280.1"
                     /translation="MRTALRAHVARAPAGGWPRAVRTACAAAALALCGASAHAGLFDD
                     DEARKAILDLRARVSQLEDTLKSHDGQNAQTLQQLQNALLDLTNQNEQLKQQIAQLRG
                     SNEQLARDVADLQKHQKDIVQGVDDRLKKVEPQQVTLDGKQFTADPDEIAAYDSATTT
                     LRNGDFPGAQAALQGFLRRWPASGYADAARYWLGNAQYGARDFKGAIATFKAFIAGAP
                     DHPRAAEAELAIANCQIELKDLKGARRTLDELVKTYPKSEAAQAGRERLSALR"
     gene            complement(4926..5462)
                     /gene="pal"
                     /locus_tag="VF453_16330"
     CDS             complement(4926..5462)
                     /gene="pal"
                     /locus_tag="VF453_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX7689281.1"
                     /translation="MKSNKSLSLVAALSVAALLGGCASGTKLNEKKAPVETAQTTTST
                     TTTPAPQSQVTPIDNTANKPAISAANLDRVIYFDFDSFAIKDEYRPIIEAHAALLKAD
                     STKKEVAEGHTDERGSSEYNLALGQKRAEAVVSQMKLLGAGDSQLEAVSYGKERPAVE
                     GHDEAAWAKNRRVELRSK"
     gene            complement(5535..6809)
                     /gene="tolB"
                     /locus_tag="VF453_16335"
     CDS             complement(5535..6809)
                     /gene="tolB"
                     /locus_tag="VF453_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX7689282.1"
                     /translation="MWPRRHALRALGASLAAPALPALAQFRVDISGVGATQIPIAIGR
                     FRGEDVLPQQLSAIVRADLANCGMFKIVDGPTPADENGSPDYNALRARGADAVASGSA
                     TRLADGTIDVRFRLWDVVKGADQGGQSEPVPLADLRLAAHHVSDKIYEKLTGDRGVFS
                     TRIAFVTHAGGKYTLFATDADGEDKKPVLISREPIISPAWSPNGKELAYVSFEQRKAV
                     LYIQNLSSGAQRVLASFRGSNSSPSFSPDGSKLTVTLSKEGGSHVFLINRDGSNPVRI
                     TQSTAIDTEAHFTPDGRSIFFVSDRGGGPQIYRQSISGGNAERVTFDGNYNVSPAPSP
                     DGKMLAYVARQSGAFKLMVMDLASGSSRGITDTSYDMNPSFAPNSRFLVYSTKVDGRD
                     ALIRTTTDGHFKTVLGDAATEYREPVWGPYGR"
     gene            7027..8796
                     /gene="msbA"
                     /locus_tag="VF453_16340"
     CDS             7027..8796
                     /gene="msbA"
                     /locus_tag="VF453_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEX7689283.1"
                     /translation="MLTLKQRLVRLLPYFRHSRLALGLAGVAMIVNAVTQPLVAKLLQ
                     LIIDRGFNGGIPLWEIPTAIVGLFVVRGTSGWVANYGLTLAAQNAVLAMRTAMFSHLM
                     RAAPALFGRSTASSLTNAVVYEIQNGASQLVGTVNTLLADSLMAVGFFVYLLILDWRL
                     TLFATFMFPATGYAVRIVGKRLRRLTAQGQTATDELAYVVEENVLAWRIVRLHAAADH
                     ERTRFGTQASRLRRLSIKQSIAGAVMDPITQLLASLSLSAVLLMAVHQGSGHRTAGEF
                     MAFITCWLGLIQPIKHLSDVMTPLTRGTAQMERGIEIIESVPAEVGGSLAPARVRGDI
                     EFRGVGLRYPAIAPPDADDAAAAAIESDRRPALSAVSLAIRAGETVAFVGPSGAGKTS
                     LVNLLPRFLEPTEGEIVLDGTPLRDYDVQALRRQFALVSQDVVLFNDTVAANVALGES
                     VDAAKVRSALAGANLLDFVEAMPGGIEATIGHNGTQLSGGQRQRLAIARAIYKDAPIL
                     ILDEATSALDSESERAVQAALEVLMKGRTTLVIAHRLSTIEHADRVVAMEHGTIAEEG
                     PHARLLAEGGLYARLHSMQFRGG"
     gene            complement(8812..>10600)
                     /locus_tag="VF453_16345"
     CDS             complement(8812..>10600)
                     /locus_tag="VF453_16345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689284.1"
                     /translation="ARPGVDGEITIDHARFCLAPLDAAAVPDCAWTTVPLAKVWHTRQ
                     RTGMGDAWYELRFELPAVPAHGLALYVPAFNRTGRLFVNGAYAQSFGSMADPLPLNWN
                     RGQFAVLSPGLLHAGVNTLAIQQRICAWENGWLATVRLGDEAHLRRVWESRRFWQNEL
                     VRVLGATTAALGAFMLAVWVHRRGQAMYFWFACASFTWTAISLDYFAQYAPLPPLAWE
                     NFVECAQVLRAVLMFMFVLRYCDRRMPRFEAAFWAWFVLGTAGIWTHLVPGRLVDDWY
                     LVVLLVSPYFFWLLVRHGLRHSRLEGVMLGLAASLQIVLSGYDLWLFSQHSWTDRIYL
                     AHFSAPVYLVVVAWVLMRRFAESLGAYERLAAVLEQRVAAKAAELERNYEQLGEARRN
                     EALALERSRIMSEMHDGIGSQLTLALSLVRSMDAQDGHAEDGRVATVLRESIEDLQLI
                     IDSLEPVENDLLTVLGTLRYRLQDRLSRSGVELQWNVVDLPPLPMLTPHSVLSILRIV
                     KEAFANCLKHAGAKRIAVATDLSGTPGSGEVARIRITDDGGGIAGTRTGRGLENMRRR
                     AEALGGRLKITSRPGCTEVMLEIPTMRGA"
BASE COUNT         1420 a         3798 c         3820 g         1562 t
ORIGIN      
        1 agcggcatgc gcgtgcgctt gtggctgccg ctggcgggcg ctgccgcatg acgctgcgca
       61 tcgcgatcgt cgacgacgag gccccggcgc gcgcgaagct gcggcgctat ctcgcggccg
      121 cgccggacgt gcacgtggtc ggcgaggccg ccagcgggcg cgaggcgctc ggcttgctgg
      181 tggagcaccg gcccgacgtc gtcttcctcg acgtgcgcat gcccgacatg gacggcttcg
      241 ccgtgctgca gtcgctcggc gagccgctgg ccatcgaggt cgtcttcgtc acggcgcacg
      301 gcgaccatgc gctgcaggcg ttcgaggtgc acgcgttcga ctacctgctc aagccggtga
      361 gccccgaacg cttcgaccgc ctgctgcggc gcctgcgcga gcgcctggtg gcgcgcgccg
      421 acgcggcagg ccgcgtcgac cgcctgctcg ccgagctgcc cgcgacggcg catcccgccg
      481 agtcgcatgc ggagcacctg ctggtcgagg ccgacggccg cggcgagctg ctgccggtcg
      541 acggcatcgt gcaggtcgag gccgaccgca actacctcgt cgtgcacgcc acggacggcc
      601 gccgcttccg cctgcgcggc acggtggagc ggatacaggc gcggctcgac ccggcgcgct
      661 tcgcccgcgt gaaccggtcg acgctggtgc gcctggccgc ggtccgcgag gtgcggccgt
      721 ggccggacgg cgagtaccgg ctgctgctcg aggacggcgg gcgcgtcacc tggacgaaac
      781 gctacctcga gcaggccgcc gcgcggctgc tgctgcggct ctgaggcgcg cgccgcgttc
      841 gtccccgcgc ggcaggcgtt cgtcccagga tcgccggcgc tggcgtgcgc ggccggcatc
      901 atgcgggcca tcgcgacacg cgcatcgaca agaggttttc gtggacagga gagatctgct
      961 caaggcggcg ctggccgcgc ccttcgccac cgggttcgca ccggcctgcg ccgcggacga
     1021 agcctccgcg acggcggcgg gggcggcggc gggctggtcg cgcgtgcgcc ccggcgacgc
     1081 cgcatggccg ggcccggacg cgtgggacgc cctctcgcgc cgcgtcggcg ggcggctggc
     1141 gaagctcgcg tcgcccttcg ccggctgcgg cggggccgac gccggcacgg cctgcggcga
     1201 cgcggcgcgc gcggcggcca acccgttctg gatcggcgac cagcccgcgc tcacccaggc
     1261 gggcggctgg gccgacgcct ggctctcgca gccgagcgcc tggggcgtcg ccgccgcgaa
     1321 cgccgccgac gtcgctgccg ccgtggactt cgcgcgcgag caccggctgc gcctggtcgt
     1381 caagggcggc ggccatggct accagggcgt gtcgtcggcg cccgattcgc tgctcgtgtg
     1441 gacgcgccac atgaacgcgg tgacgctgca cgacgccttc gtcgcccgcg gctgcgacgc
     1501 cgcgccgcag ccggccgtca gcctcggcgc cggcgcgatg tggatcgacg cctacgacgc
     1561 ggtcaccacc cgcgccggcc gctacgtgca gggcggcggc tgcaccacgg tcggcgtcgc
     1621 gggcctcgtg cagagcggcg gcttcggcag cttctccaag cgcttcggca ccgccgcggc
     1681 gggcctgctg gaggccgagg tcgtgaccgc cgacggccag gtgcgcgtcg tcaacgcctg
     1741 cagccatccc gacctgttct gggcgctcaa gggcggcggc ggcggcagct tcggcgtcgt
     1801 gacccgggtc acgctgcgca cgcacgagct gccggagacc ttcggtgccg tgcgcggcac
     1861 gatacgggcg aaggacgaca ccgcgttccg cgcgctgctg gcgcgggtcg tcgacttcca
     1921 cgccgacgtc ctgcacaacc cgcactgggg cgagcagatc ggcctgcgca cggacaacag
     1981 cctgcacttc gcgctcgcgt tccagggcct gacgcaggcc caggccgagg aggcctggcg
     2041 gccgctgtgc gagtggctct ccgcgtacgc ggatgccctg gaggtcgtcg agccgatcgg
     2101 gatccacgcc ctgcccccgc gccgctactg ggacgccgcg ttctggcgcc gcgtcgcgcc
     2161 cggcgtcatg gtttgcgacg accgccccgg cgcgcccccg caccatgccg agtgggccgg
     2221 cgaccgcaag caggccggca tgttcctgca cggctacgag tcggcctggc tgcccgcgcg
     2281 gctgctggcg cacgaggagc gcccgcgcct ggtcgacgcc ctgttcgccg cgacgcgcca
     2341 cttcgaggtc ggcctgcact tcaacaaggg cctggccggc gccgcggacg ccgagatcgc
     2401 cgccgcgcgc gacaccgcca ccaatcccgc ggtgctggac gccttcgcgc tcgcgatcat
     2461 cgcgacggcg gggtcgtcgg cctggcccgg cgtcgcgccc caggcgcccg acctggccga
     2521 ggcccgccgc caccgcgccg ccatcggcct cgcgatgacc gaactgaaga aggcggcgcc
     2581 cggcgcgggc tcctacgtct ccgagagcga ctacttcctg cgcgactggc cgaccgcgtt
     2641 ctggggtgcg aaccacgcgc gcctcgcggc cgtgaagcaa cgctacgacc cgcagggcct
     2701 gttcttcgtg cggcacggcg tgggcagcga ggcgtggagc gacgacggct tcacgcggat
     2761 cgccgcgtcg cagccgcagg cctgagcctc gccaagcgtc tcggccccgc aaggggcaag
     2821 aaaaaagcac ccgccgctcg cgcgccgggt gcttgtttca ttaccagatc tggtgggccc
     2881 tgagtgactc gaacactcga ccaacggatt aagagtccgc tgctctacca actgagctaa
     2941 gagcccgaaa tcttcgattt cttcttcttc gaattcgact tgcagtcgtc aaactgcaag
     3001 ccttgcattc tacttcgctt ccgaaacgac tcgcaagcaa aacccgcgtt ctcgctcgga
     3061 aaaccttccg gcgaacaagc cccgggaggt ggtgggttgt acaggacttg aacctgtgac
     3121 caacggatta aaagtccgct gctctaccga ctgagctaac aacccgtttc cggcagagcc
     3181 ataaatgtta gcatgcctgc ccgctgtcgc gcaaggccgt cgatggcctg agcgtcacgg
     3241 cggaccgggt cgacggcctt cgccgcgatc agctgggcga tgacctcgcc cgccgccgcc
     3301 aggccgaagg tggccgtgac ggtcaccgtc gacccgtagc cgtggcagtt gagcgagttg
     3361 tcgcgccgga ccgcggacgg atcgacgtcg cacgcggcgt cctcgtcgtc gaccgcgggt
     3421 ggggcgacgc tctcgcgcga gaacacgcag cgcaccccca tgcgtccgct gcgcggcgcg
     3481 ccgtggcggg cgcgcaggcg ctggcgcagg ccggccagca gcgggtcgtg cgtcgtggcg
     3541 tggaggtcgt cgatctccac cttctccgcg cgctgcttgc cccccgccgc gccacaggtg
     3601 acgaacggca tgcacgtgcg gcgcgcccag tcggccagca tcagcttggc gcggctctgg
     3661 tcgcaggcgt cgatgaccgc gtcgacctcg accggcagca ggccgggcca gttggtctcg
     3721 tcggcgaact cctcgacgcc gtgcacgcgg cagccgggat ggatgtcggc gatgcgccgg
     3781 gccagcgcct cggccttgga catgccgatc gtcgcgccga cggcctgcac ctggcgattg
     3841 acgttggact ccgcgacatg gtcgaggtcg accagcacca gctgcgcgac gccgctgcgc
     3901 gcgagcgcct cgaccgccca cgagccgacg ccgcccaggc ccacgaccgc cacccgcgcg
     3961 gcgcgcacac gcgcgtaccc cgccgcgccg tacaggcgct tcaggccgcc gaagcggcgt
     4021 tccaggtcgg cctcgagcgg gaagtcgtcg acgaccggcc cggtcgccga cgatgcggca
     4081 gcggtggacg acaccggtgt cccgcgccgc ctacctcagc gccgagagcc gctcgcgacc
     4141 cgcctgcgcc gcttccgact tcgggtaggt cttgaccagc tcgtcgagcg tccggcgggc
     4201 acccttgaga tccttcagct cgatctggca gttcgcgatc gcgagctcgg cctccgcggc
     4261 gcgcgggtgg tcgggcgcgc cggcgatgaa ggctttgaag gtggcgatgg cgcccttgaa
     4321 atcgcgtgcg ccgtactgcg cgttgccgag ccagtagcgc gccgcgtccg cgtagccgct
     4381 ggccggccag cggcgcagga agccctgcag cgcggcctgg gcgccgggga agtcgccgtt
     4441 gcgcagcgtg gtggtcgcgc tgtcgtaggc ggcgatctcg tcggggtcgg ccgtgaactg
     4501 cttgccgtcg agcgtgacct gctgcggctc gaccttcttc agccggtcgt ccacgccctg
     4561 gacgatgtcc ttctggtgct tctggagatc ggcgacgtcg cgcgccagct gctcgttgga
     4621 gccgcgcagc tgggcgatct gctgcttgag ctgctcgttc tggttggtca ggtcgagcag
     4681 cgcgttctgc agttgctgca gcgtctgcgc gttctggccg tcgtgcgact tcagcgtgtc
     4741 ctcgagctgc gagacgcgcg cccgcaggtc gaggatggcc ttgcgggcct cgtcgtcgtc
     4801 gaacaggccg gcatgcgcgc tcgcgccgca cagcgcgagg gccgcggcgg cgcacgcggt
     4861 gcgcaccgcg cgcggccatc cacccgcggg cgcgcgggcg acgtgggcac gaagcgcggt
     4921 ccgcatcact tgctgcgcag ctcgacgcgg cggttcttcg cccacgcggc ctcgtcatgg
     4981 ccctcgacgg ccgggcgctc cttgccgtag ctgacggcct cgagctggct gtcgccggcg
     5041 cccaggagct tcatctggct gaccaccgcc tcggcgcgct tctggccgag ggcgaggttg
     5101 tattcgctgc tgccgcgctc gtcggtgtgg ccctcggcga cttccttctt cgtgctgtcg
     5161 gccttcagca gggcggcgtg ggcctcgatg atcgggcggt actcgtcctt gatcgcgaag
     5221 ctgtcgaagt cgaagtagat gacgcggtcg aggttggcgg cggagatcgc gggcttgttg
     5281 gccgtgttgt cgatcggcgt gacctggctc tggggcgccg gcgtcgtcgt cgtggacgtg
     5341 gtggtctgcg cggtctcgac cggcgccttc ttctcgttga gcttggtgcc cgacgcgcag
     5401 ccgcccagca gcgcggcgac ggacagcgcg gcgaccaggg acagggattt gttcgacttc
     5461 atcaggattc tccttccagc actatgaaaa agccgggcgg cggcgctgct gcgcgctccg
     5521 gcacttgccc gagatcagcg gccgtacggc ccccataccg gttcgcgata ttcggtggcc
     5581 gcatcgccaa gcaccgtctt gaagtgcccg tcggtcgtgg tcctgatcag cgcgtcgcgg
     5641 ccgtccacct tcgtcgaata caccaggaag cgcgagttcg gcgcgaagct gggattcatg
     5701 tcgtaggagg tgtcggtgat gccgcggctc gagccgctgg ccagatccat caccatcagc
     5761 ttgaacgcgc cgctctggcg cgcgacgtag gcgagcatct tgccatccgg gctcggcgcg
     5821 gggctgacgt tgtagttgcc gtcgaaggtc acgcgctcgg cgttgccgcc ggagatcgac
     5881 tggcggtaga tctgcgggcc gcctccgcgg tcgctcacga agaagatgct gcgcccgtcg
     5941 ggggtgaagt gcgcttccgt gtcgatcgcc gtgctctggg tgatgcgcac cgggttgctg
     6001 ccgtcgcggt tgatcaggaa gacgtgggag ccgccctcct tggacagcgt gacggtgagc
     6061 ttggagccgt ccggcgagaa cgagggcgag ctgttggagc cgcggaagct cgcgagcacc
     6121 cgctgcgcgc ccgaggacag gttctggatg tagagcaccg ccttgcgctg ctcgaacgag
     6181 acgtacgcca gctccttgcc gttcggcgac caggccggcg agatgatggg ctcgcggctg
     6241 atcagcaccg gcttcttgtc ctcgccgtcg gcatcggtcg cgaacagcgt gtacttgccg
     6301 cccgcgtgcg tgacgaaggc gatgcgcgtg gagaagacgc cgcggtcgcc ggtgagcttc
     6361 tcgtagatct tgtcggagac gtggtgggcc gcgaggcgca ggtcggcgag cggcacgggc
     6421 tcggactggc cgccctggtc ggcgcccttg acgacgtccc agagccggaa gcgcacgtcg
     6481 atcgtgccgt cggccaggcg ggtggcggag ccggaagcca ccgcgtccgc accgcgcgcg
     6541 cgcagggcgt tgtagtcggg cgagccgttc tcgtcggccg gcgtcgggcc gtcgacgatc
     6601 ttgaacatcc cgcaattggc gaggtcggcg cggacgatcg ccgacagctg ctggggcagg
     6661 acgtcctcgc cccggaagcg tccgatcgcg atcgggatct gggtggcgcc gacgccggag
     6721 atgtcgaccc ggaactgggc gagggccggc agtgccggcg cggcgagcga ggcgccgagg
     6781 gcgcgcaggg catgtcttct aggccacatg gcgatgcagg agtcctgtcg ggcagaaagg
     6841 ttccggagaa attgtccgct caactcggcg gtacgcgggc aggcgggatt gtaggcagca
     6901 agacggatgc ctgctgtagg cgaaccggcc gtccttgcgg cgatccggcg tccgggggcc
     6961 cggcgcgcgc gcagataatg cgcggctgga ctccccgcga cggcgccgcc ccgcccctcc
     7021 tgcccgatgc tgaccctgaa gcagcgcctc gtgcgcctgt tgccctactt ccgccacagc
     7081 cggctcgccc tcggcctggc gggcgtcgcg atgatcgtca acgccgtcac ccagccgctg
     7141 gtggccaagt tgctgcagct catcatcgac cgtggcttca acggcggcat cccgctgtgg
     7201 gagatcccga cggcgatcgt ggggctcttc gtcgtgcgcg gcacctcggg ctgggtcgcc
     7261 aactacgggc tgacgctcgc cgcgcagaac gccgtgctcg cgatgcgcac ggcgatgttc
     7321 tcgcacctca tgcgcgccgc gccggccctg ttcgggcgca gcaccgcgtc gagcctgacc
     7381 aacgcggtcg tctacgagat ccagaacggc gcgagccagc tggtgggcac cgtcaacacg
     7441 ctgctggccg actcgctgat ggccgtcggc ttcttcgtct acctgctgat cctggactgg
     7501 cggctcacgc tgttcgcgac cttcatgttc ccggccaccg gctacgcggt gcgcatcgtc
     7561 ggcaagcgtc ttcggcggct gacggcccag gggcagacag cgaccgacga gctcgcctac
     7621 gtcgtcgagg agaacgtgct cgcgtggcgc atcgtgcgcc tgcacgccgc agccgatcac
     7681 gagcgcaccc gcttcggcac ccaggcgagc cgcctgcgcc ggctgtcgat caagcagtcg
     7741 atcgccggcg cggtgatgga tccgatcacc cagctgctcg cgtcgctgtc gctgtcggcc
     7801 gtgctgctga tggcggtgca ccagggctcg ggacatcgca cggcgggcga gttcatggcc
     7861 ttcatcacct gctggctggg cctgatccag ccgatcaagc acctgtccga cgtgatgacg
     7921 ccgctcaccc gcggcacggc gcagatggag cgcggcatcg agatcatcga gtcggtgccg
     7981 gcggaggtcg gcggcagcct cgcgcccgcg cgcgtgcggg gcgacatcga gttccgcggc
     8041 gtcggcctgc gctatcccgc catcgccccc cccgacgccg acgatgccgc cgccgcggcc
     8101 atcgagtccg accgccgccc cgcgctgtcg gcggtgtcgc tggcgatccg cgcgggcgag
     8161 accgtcgcct tcgtcggccc ctcgggcgcc ggcaagacct cgctcgtcaa cctgctgccg
     8221 cgattcctgg agcccaccga gggcgagatc gtcctcgacg gcacgccgct gcgcgactac
     8281 gacgtgcagg cgctgcgccg ccagttcgcg ctcgtgagcc aggacgtcgt gctgttcaac
     8341 gacaccgtcg ccgccaacgt ggcgctgggc gagtcggtcg acgcggccaa ggtgcgcagc
     8401 gcgctggccg gcgccaacct gctcgatttc gtcgaggcga tgcccggcgg catcgaggcg
     8461 acgatcggcc acaacggcac ccagctctcc ggcggccagc gccagcgcct ggcgatcgcc
     8521 cgcgcgatct acaaggacgc gccgatcctg atcctcgacg aggcgacgtc cgcgctcgac
     8581 tcggagtccg agcgcgccgt ccaggccgcc ctcgaggtgc tcatgaaggg ccgcaccacg
     8641 ctggtcatcg cccaccgcct gtcgaccatc gagcacgccg accgcgtcgt cgcgatggaa
     8701 cacggcacca tcgccgagga agggccgcac gcgcggctgc tcgccgaggg agggctgtat
     8761 gcgcggctgc attcgatgca gttccgcggg gggtgagcgc cgcctgcgcg ctcaggcccc
     8821 ccgcatcgtc gggatctcca gcatcacctc ggtgcagccc ggccgcgacg tgatcttcag
     8881 ccgcccgccg agcgcctcgg cgcggcggcg catgttctcg aggccgcggc cggtgcgcgt
     8941 accggcgatg ccgccgccgt cgtcggtgat gcggatgcgg gcgacctcgc cggagccggg
     9001 cgtgccggag aggtcggtgg ccacggcgat gcgcttcgcg cccgcatgct tgaggcagtt
     9061 ggcgaaggcc tccttcacga tgcgcaggat ggacagcacg ctgtgcggcg tgagcatcgg
     9121 cagcggcggc aggtcgacga cgttccactg cagctcgacg ccgctgcggc tcaggcgatc
     9181 ctgcagccgg tagcgcagcg tgcccagcac ggtgagcagg tcgttctcga ccggctcgag
     9241 cgagtcgatg atcagctgca ggtcctcgat gctctcgcgc agcaccgtcg cgacgcggcc
     9301 gtcctcggcg tggccgtcct gcgcgtccat gctgcgcacg agcgacagcg ccagcgtcag
     9361 ctgcgagccg atgccgtcgt gcatctcgct catgatgcgc gagcgctcga gggccagcgc
     9421 ctcgttgcgg cgcgcctcgc cgagctgctc gtagttgcgc tccagctccg cggccttcgc
     9481 ggcgacgcgc tgctccagca cggcggcgag tcgctcgtag gcgccgagcg actcggcgaa
     9541 gcgccgcatc agcacccacg cgaccacgac gaggtagacc ggcgcgctga agtgcgcgag
     9601 gtagatccgg tcggtccagc tgtgctgcga gaacagccac aggtcgtagc cgctcagcac
     9661 gatctgcagg ctcgccgcca ggcccagcat cacgccctcg agccggctgt ggcgcaggcc
     9721 gtggcgcacc agcagccaga agaagtacgg cgagaccagc agcacgacga ggtaccagtc
     9781 gtcgacgagg cgtcccggca ccaggtgcgt ccagatgccc gcggtgccga ggacgaacca
     9841 ggcccagaac gcggcctcga agcgcggcat gcgccggtcg cagtagcgca ggacgaacat
     9901 gaacatcagc accgcgcgca gcacctgggc gcactcgacg aagttctccc acgcgagcgg
     9961 cggcagcggc gcgtactgcg cgaagtagtc gaggctgatg gccgtccacg tgaacgaggc
    10021 gcaggcgaac cagaagtaca tcgcctgccc gcgccggtgg acccagacgg ccagcatgaa
    10081 ggcgcccagc gcggccgtcg tcgcgccgag gacgcgcacg agctcgttct gccagaagcg
    10141 gcgcgactcc cagacccggc gcaggtgcgc ctcgtcgccc aggcgcaccg tcgcgagcca
    10201 gccgttctcc caggcgcaga tgcgctgctg gatcgcgagc gtgttgacgc ccgcgtgcag
    10261 cagccccggc gacagcacgg cgaactggcc gcggttccag ttcagcggca gcgggtcggc
    10321 catcgacccg aaggactgcg cgtacgcgcc gttgacgaac agccgcccgg tgcggttgaa
    10381 cgccggcacg tacagcgcca ggccgtgcgc cggcacggcg ggcagctcga agcgcagttc
    10441 gtaccacgcg tcgcccatgc cggtgcgctg ccgcgtgtgc cagaccttgg cgagcggcac
    10501 ggtcgtccac gcgcaatcgg ggacggcggc tgcgtcgagc ggcgcgaggc agaagcgcgc
    10561 atggtcgatg gtgatctcgc cgtcgacgcc gggccgcgcc
//
LOCUS       DASVLN010000692         4198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_410545, whole genome shotgun sequence.
ACCESSION   DASVLN010000692 DASVLN010000000
VERSION     DASVLN010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410545"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..736)
                     /locus_tag="VF453_16350"
     CDS             complement(128..736)
                     /locus_tag="VF453_16350"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX7689285.1"
                     /translation="MTAPSPRRRPQRRGIVAEHSNGSALLILDMISRWDYPDGATLLR
                     HALRVAPAIAALRMRCRERDVPVIYANDNSGQWRSDFKFVVREALESPGGGARIAQML
                     EPGDEDYFVLKPKHSAFFATPLELLLDHLRVRRLVVVGVTSDQCVAATAADARMRDFE
                     LVIPEDAVATITAARNRRALAHIREVLEVSTTPAARVRLDAA"
     gene            961..1845
                     /locus_tag="VF453_16355"
     CDS             961..1845
                     /locus_tag="VF453_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7689286.1"
                     /translation="MRTAVGDVRVGLSGWRYASWRGPFYPAGLAQRRELEFASRAFGT
                     LEINGSFYSLQSPRSWHAWRDETPAGFRFAVKGPRYVTHLLKLRNAQAAIANFFASGV
                     LALGDKLETVLWQLPPQLGFDEERLEGFLAALPHDTGHARALAAGHDERMAGRAWFEP
                     APVRPLRHALEVRHPGFEVPACVALLRRHGVALVVADTAGLYPRLEDVTADLVYVRLH
                     GDTALYRSGYGDAALADWARRIEAWAAGGQVDDARVVSRRRPARRPRDVRVYFDNDTD
                     AHAPRDAARLATILGPAR"
     gene            1931..2119
                     /locus_tag="VF453_16360"
     CDS             1931..2119
                     /locus_tag="VF453_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEX7689287.1"
                     /translation="MSDDKTNSHGQDRERINLGEDYEVRDWSRRFGISADELRAAVQA
                     VGDRAAAVEAWVKGHAQR"
     gene            2124..2936
                     /locus_tag="VF453_16365"
     CDS             2124..2936
                     /locus_tag="VF453_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7689288.1"
                     /translation="MRVATWNVNNVNRRLEQLLDWMARTAPDVVALQELKAETADFPA
                     QALRDAGYESVAVGQRTWNGVALLARGRAPVPVRRALPGEPEDTQARYVEAAVDGILF
                     GSLYLPNGNPRPGPKFDYKLRWFERLRRHAAELLATGEPVVLLGDWNVVPTDADIYRP
                     DHWRDDALLQPEPRQAYADVLAQGWTDALRHRHPEDPPPFTFWDYRRKRWERDAGLRI
                     DHILASPALEVVDAGVDRALRGVDHPSDHAPVWAELRRAAPRQRARAGARKA"
     gene            2933..>4198
                     /locus_tag="VF453_16370"
     CDS             2933..>4198
                     /locus_tag="VF453_16370"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7689289.1"
                     /translation="MSGAHRRRRQQGAGRLARRPRAVAAASALALGCAITPAACAQAS
                     AAAEQSVTVTATRLAAPTFDIGASVDRVDVPQADADRPGVNLSEVLAGVPGVLARDRQ
                     NYAQDMQLSIRGFGARSSFGIRGIRLYVDGIPATMPDGQGQLSNVDLGSAAAIEVLRG
                     PFSALYGNASGGVVQVFTRTGRGAPVLDVGMAVGSDGQRRERTRLEGSTGAFDYDLSA
                     SHFQTRGLREHGAARRDVQNAKLGWQLDADTHVTLVGNAVDLPEAQDPLGLTRAEMEA
                     DPRGVDPSAIAYDTRKSLRQSQGGLVLDRRVDADDDVHALVYGGQRRTTQFQAIPARA
                     QANPASPGGVIVLARGYGGAALRWTHRGMLLGAPATLVGGIDADRLLEHRQGLLNFTG
                     TPDDPTPGVAGALRRDGTNLVTSADPYVQL"
BASE COUNT          542 a         1580 c         1562 g          514 t
ORIGIN      
        1 atccgcgccg cgaccgggcg ggcgactcgg cggccggttc gctcgccggc ggcggcaagt
       61 ccgggcacgc cggcgcacgg accagcaggg agcgcgctgg cgcgacggct tttgctgaaa
      121 ggagcggtca cgccgcatcg agcctcaccc gtgccgcggg cgtggtcgac acctcgagca
      181 cctcgcggat gtgcgcgagc gcgcggcggt tgcgtgccgc ggtgatggtc gcgacggcgt
      241 cctccgggat gacgagctcg aagtcgcgca tgcgcgcgtc cgcggcggtg gcggcaacac
      301 actggtccga ggtcacgccg acgacgacca gccgccgcac gcgcaggtga tcgagcagca
      361 gctccagcgg cgtcgcgaag aacgcggagt gcttgggctt gaggacgaag tagtcctcgt
      421 cgccgggctc cagcatctgc gcgatgcggg caccgccgcc gggcgattcc agcgcctcgc
      481 ggacgacgaa cttgaagtcc gatcgccact gcccgctgtt gtcgttggcg tagatgacgg
      541 gcacatcgcg ctcgcggcag cgcatgcgca gcgcggcgat ggccggcgcg acgcgcagcg
      601 cgtggcgcag cagcgtcgcg ccgtcgggat agtcccagcg gctgatcatg tccaggatca
      661 gcagcgcact gccgttggag tgttcggcga cgatgccgcg gcgttgcggg cggcgtcttg
      721 gcgagggagc ggtcatgacc ggggtcgagc aacgacgatg ccggcggcgg gccggtggct
      781 tgccgaaggg cccacccttc atcaccaggg aggcccggcc ttcccgcagc accccatgcc
      841 cgccactcga agccgtccgc cgctcgacca cccgcgcccc gacgctcgaa gcgcgcctgc
      901 gcgcgcgcgt ccgacttcct cgcgggcgcc gcggcgcacg ggcgcccgca cctcgccgcg
      961 atgaggacgg ccgtcggcga cgtgcgggtc ggcctctcgg gctggcgcta cgccagctgg
     1021 cgcggcccgt tctatccggc cggcctcgcg cagcggcgcg agctcgagtt cgcgtcgcgc
     1081 gccttcggca cgctggagat caacggctcg ttctactcgc tgcagtcgcc gcgctcctgg
     1141 cacgcgtggc gcgacgagac gcccgcgggc ttccgcttcg ccgtcaaggg cccgcgctac
     1201 gtgacgcacc tgctcaagct gcgcaacgcg caggcggcca tcgccaactt cttcgcctcg
     1261 ggcgtgctgg cgctcgggga caagctcgag accgtgctct ggcaactgcc gccgcagctg
     1321 gggttcgacg aggagcggct ggagggcttc ctcgccgcgc tgccgcacga cacgggccat
     1381 gcgcgcgcgc tcgccgcggg ccacgacgag cgcatggccg ggcgcgcctg gttcgagccc
     1441 gcgccggtgc gcccgcttcg ccacgcgctg gaggtgcgcc atccgggctt cgaggtgccc
     1501 gcctgcgtcg cgctgctgcg gcggcacggc gtggcgctgg tcgtcgcgga taccgcgggg
     1561 ctgtatccgc gcctggagga cgtgacggcc gacctcgtct acgtccgcct gcacggcgac
     1621 accgccctct accgcagcgg ctacggcgac gcggcgctgg ccgactgggc gcgccgcatc
     1681 gaggcctggg ccgcgggcgg gcaggtcgac gacgcgcgcg tcgtctcgcg ccgaaggccc
     1741 gcgcgtcggc cccgcgacgt gcgggtgtac ttcgacaacg acaccgacgc ccacgcgccg
     1801 cgggacgccg cgcgcctcgc cacgatcctc ggcccggcgc gctgaccacc gccgggcagg
     1861 cttcttgctc gtccggcatc gagccacctg ccgatggccg cgcccttgac ctcacacgaa
     1921 aggagacccc atgtcggacg acaagacgaa cagccacgga caggaccgcg agcgcatcaa
     1981 cctgggcgag gactacgagg tccgcgactg gagccgcagg ttcggcatct ccgcggacga
     2041 gctgcgcgcg gccgtccagg ccgtggggga ccgggcagca gcggtcgagg cctgggtcaa
     2101 gggccacgcc cagcgctgac accgtgcggg tcgcgacctg gaacgtcaac aacgtcaacc
     2161 ggcggctgga gcagctcctg gactggatgg cgcgcacggc gccggacgtc gtcgcgctcc
     2221 aggagctcaa ggccgagacg gcggacttcc ccgcgcaggc gctgcgcgac gcgggctacg
     2281 agtcggtcgc ggtcggccag cgcacctgga acggcgtcgc gctgctcgcg cggggccgcg
     2341 cgccggtgcc ggtgcggcgc gccttgccgg gcgagcccga ggacacgcag gcacgctacg
     2401 tcgaggccgc cgtcgacggc atcctcttcg gcagcctgta cctgcccaac ggcaacccgc
     2461 gccccgggcc gaagttcgac tacaagctgc gctggttcga gcgcctgcgc cggcacgccg
     2521 cggagctgct ggccaccggc gagccggtcg tgctgctggg cgactggaac gtcgtgccga
     2581 ccgacgccga catctaccgc cccgaccatt ggcgcgacga cgcgctgctg cagcccgagc
     2641 cgcgccaggc ctacgccgac gtgctcgcgc agggctggac ggacgcgctg cgccatcgtc
     2701 atcccgagga tccgccgccc ttcaccttct gggactaccg ccgcaagcgc tgggagcgcg
     2761 acgcgggcct gcgcatcgac cacatcctcg cgagcccggc gctggaggtc gtggacgccg
     2821 gcgtcgaccg cgcgctgcgc ggcgtcgacc atccgagcga ccacgcgccc gtctgggccg
     2881 agctgcggcg ggcggctccg cgccagcgcg cgcgcgccgg ggcccgcaag gcatgagcgg
     2941 ggcgcaccgc agacggcggc agcaaggtgc cgggcggctc gcgcgccgac cccgtgccgt
     3001 cgcggcggca tccgcgctgg cactcggctg cgcgatcacg cccgcggcct gcgcgcaagc
     3061 gtctgcggcg gcggagcagt ccgtcaccgt caccgcgacg cgcctggccg cgcccacctt
     3121 cgacatcggc gcctcggtcg atcgcgtgga cgtgccgcag gccgacgccg accgcccggg
     3181 cgtcaacctg tccgaggtgc tggccggcgt gccgggcgtg ctcgcccgcg atcgccagaa
     3241 ctacgcgcag gacatgcagc tgtcgatccg cggcttcggc gcgcgctcga gcttcggcat
     3301 ccgcggcatc cggctgtacg tcgacggcat cccggcgacg atgccggacg gccagggcca
     3361 gctgtccaac gtcgacctcg gctcggccgc ggccatcgag gtgctgcgcg ggccgttctc
     3421 ggcgctgtac ggcaacgcct cgggcggcgt cgtgcaggtc ttcacgcgca cgggccgcgg
     3481 cgcgccggtg ctggacgtcg gcatggccgt gggcagcgac ggccagcggc gcgagcggac
     3541 ccggctcgag ggctccacgg gcgcgttcga ctacgacctg agcgcctcgc acttccagac
     3601 ccgcggcttg cgcgagcacg gcgccgcgcg gcgggacgtg cagaacgcca agctcggctg
     3661 gcagctcgac gccgacacgc acgtcacgct ggtcggcaac gccgtcgacc tgcccgaggc
     3721 gcaggatccg ctggggctga cgcgcgccga gatggaggcg gatccccgcg gcgtcgaccc
     3781 gtcggcgatc gcgtacgaca cgcgcaagtc gctgcgccag tcgcagggcg ggctcgtcct
     3841 cgaccgccgc gtggatgccg acgacgacgt gcacgcgctc gtctacggcg gccagcggcg
     3901 cacgacgcag ttccaggcga tcccggcgcg cgcgcaggcc aatccggcga gtccgggcgg
     3961 cgtcatcgtg ctggcgcgcg gctacggcgg cgcggcgctg cggtggacgc accggggcat
     4021 gctgctcggc gcgccggcga cgctggtcgg ggggatcgac gccgaccggc tgctcgagca
     4081 tcggcagggc ctgctgaact tcaccggcac gcccgacgac ccgacgccgg gcgtcgcagg
     4141 cgcgctgcgt cgcgacggca cgaacctcgt cacctccgcc gacccgtacg tgcagttg
//
LOCUS       DASVLN010000693         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_34870, whole genome shotgun sequence.
ACCESSION   DASVLN010000693 DASVLN010000000
VERSION     DASVLN010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34870"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..654
                     /locus_tag="VF453_16375"
     CDS             157..654
                     /locus_tag="VF453_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7689290.1"
                     /translation="MYARILVPVDASPTAEAGVAEALRIARACGARVLFVHAVDQLPH
                     LPEAVAYEQAPEDVASAAVERGLDLLERLREQAQEAGVAVDAELVEGLGRRTHAFVLE
                     RAADWRADLVVLGTHGRRGIERWIAGSVAEQVVRHADVPVLLVRCPSQEPAGEGLQSI
                     AAAIA"
     gene            complement(679..1527)
                     /locus_tag="VF453_16380"
     CDS             complement(679..1527)
                     /locus_tag="VF453_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7689291.1"
                     /translation="MTYPTVLLHLDDDVRSDVRNEQAVRFAKRHGSHVDGLSCHRPVP
                     TGSGFAVSMGGITDTLTDELQRAQRQAAEREQRFRALCDRQGLASFDTEVDEAEEASR
                     AILRRAPLHDLVVLGQPDPSEPDAARRREVLEEVVLHNPRPTLLLPYAGRFDDIGRTV
                     LLAWDDSHGAARAAADALPLLKRADAVHLVTFDRDAGESGVIDQSPVRGVLCWLGRHG
                     VSANGGVRFVAADVGNALLSHAADVGADVLVMGSWGHARWAERLIGGATRTVLASMTL
                     PVLTSR"
     gene            1735..2049
                     /locus_tag="VF453_16385"
     CDS             1735..2049
                     /locus_tag="VF453_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEX7689292.1"
                     /translation="MTTRRAFIQILPLAGAAALCANAALAAGAAPTLDPKDAQAVALG
                     YVVDATKADKAKYPKYAAGQACGSCQLYQGKAGDAAAPCALFGGKQVGAKAWCSAYVK
                     KA"
     gene            2063..2647
                     /locus_tag="VF453_16390"
     CDS             2063..2647
                     /locus_tag="VF453_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7689293.1"
                     /translation="MRTALRTLREEHAALAALLQTLLLMTAQARAGGHAPDFMAMRAM
                     LFYVAEFPDKRHHVKESQLLFPRLRARTPLAREVLDRLDRDHDCGHARIRELEHALTA
                     YEMMGPVRAEDFERALRRYVEFHRVHMTLEEDAVLPLAERWLTDEDWRHLDQELAGEP
                     DPLTGRRADDEYRLLFERIVDLVPAPFGFGQAAR"
     gene            complement(2724..>2979)
                     /locus_tag="VF453_16395"
     CDS             complement(2724..>2979)
                     /locus_tag="VF453_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEX7689294.1"
                     /translation="VVLSPAALAHRSAVASLVCHGRIDFEAIALAHLVEPRLCFARAL
                     RELAPLVRAGLVRMDDDGVELTPPGEHLVEVVAGAFEPMA"
BASE COUNT          400 a         1135 c         1091 g          353 t
ORIGIN      
        1 gtgggcgatc cggccacgat aggggcgcgc ggcggccgcg cgttgagcca ccgcaagcgg
       61 gcgccgcgcc gccgtcgcgg cgaccttgac gcgcctcaac gcccgcgccg gcgcgacgcc
      121 gagactcgac ccatccgcca cgcccgggag caagccatgt acgccaggat cctcgtgccc
      181 gtcgacgcca gcccgaccgc ggaggccggc gtcgccgaag cgctgcgcat cgcccgggcc
      241 tgcggcgccc gggtgctgtt cgtccacgcc gtcgaccagc tgccccacct gcccgaggcc
      301 gtggcctacg agcaggcgcc ggaagacgtc gcctcggcgg cggtggagcg cggcctcgac
      361 ctgctggagc gcttgcgcga gcaggcgcag gaggccggcg tggccgtgga cgccgagctg
      421 gtcgagggcc tcggacggcg cacccatgcc ttcgtgctcg aacgcgccgc cgactggcgc
      481 gcggatctcg tcgtcctggg cacgcacggc cggcgcggca tcgagcgctg gatcgcgggc
      541 agcgtcgccg agcaggtcgt gcgtcacgcc gacgtgcccg tgctgctggt gcgctgcccg
      601 tcgcaggagc cggcgggcga aggcctgcaa tcgatcgcgg ccgcgatcgc ctgaggcgac
      661 cgcgacacgc ttgcggcctc agcgcgaggt cagcaccggc agcgtcatgc tcgcgagcac
      721 cgtgcgcgtg gcgccgccga tcaggcgttc ggcccagcgg gcgtggcccc agctgcccat
      781 cacgaggacg tcggcgccga catcggcggc atgcgacagc agcgcgttgc cgacgtcggc
      841 ggcgacgaag cgcacgccgc cgttcgcgga cacgccgtgc cggcccagcc agcacagcac
      901 gccgcgcact ggcgactggt cgatcacgcc cgactcgccg gcgtcgcggt cgaaggtcac
      961 gaggtgcacc gcgtccgcgc gcttcagcag cggcagcgcg tcggcggccg cgcgggcggc
     1021 gccgtggctg tcgtcccacg cgagcagcac ggtgcgtccg atgtcgtcga agcggcccgc
     1081 gtagggcagc agcagcgtcg ggcgcgggtt gtgcagcacg acttcctcga ggacctcgcg
     1141 ccggcgcgcg gcgtcgggct ccgacgggtc cggctgcccg agcaccacga ggtcgtgcag
     1201 cggcgcgcgc cgcaggatcg cacggctcgc ctcctcggcc tcgtcgacct cggtgtcgaa
     1261 cgacgccagc ccctggcggt cgcacagcgc gcggaaccgc tgctcgcgct cggcggcctg
     1321 gcgctgcgcg cgctgcagct cgtcggtcag ggtgtcggtg atgccgccca tggacaccgc
     1381 gaacccggag ccggtgggca ccgggcgatg gcaggacagc ccgtcgacat ggctgccatg
     1441 gcgcttggcg aagcggacgg cctgctcgtt gcggacgtcg gaccggacgt cgtcgtccag
     1501 gtgcaggaga acggtcggat aggtcatgcg ggactcccgt cgaggtagag accggcgaat
     1561 tctgcgaacg cccgcgccgc cggcgcttgc ggcatcgcaa aggcgccgcg ggcgccgtcg
     1621 cccccgcggc cgcgacgccg tctgatcgag atcaagcgcg gtgcgtgtcc gcgccgctaa
     1681 cgtggcaacg ccgcgccatc ccggccggca tccgaacccc cgaggaccca cgagatgacc
     1741 acccgccgcg ccttcatcca gatcctgccg ctcgccggcg ccgccgcgct gtgcgccaac
     1801 gccgcgctcg cggccggcgc ggcgcccacg ctcgacccca aggatgcgca ggcggtcgcg
     1861 ctcggctacg tcgtcgacgc gaccaaggcc gacaaggcca agtacccgaa gtacgcggcc
     1921 ggccaggctt gcggcagctg ccagctgtac cagggcaagg ccggcgacgc cgccgcgccc
     1981 tgcgcgctgt tcggcggcaa gcaggtcggc gcgaaggcgt ggtgcagcgc ctacgtgaag
     2041 aaggcctgac gcgggcgtcg cgatgcgcac cgccctgcgt acgctgcggg aggagcatgc
     2101 cgccctggcc gccctgctgc agacgttgct gctgatgacg gcgcaggcgc gtgccggcgg
     2161 gcatgcgccc gacttcatgg cgatgcgcgc gatgctgttc tacgtcgcgg agttccccga
     2221 caagcgccac cacgtgaagg aatcgcagtt gctgttcccg cgcctgcgcg cccgcacgcc
     2281 gctcgcccgc gaggtgctgg accgcctcga ccgcgaccac gactgcggcc atgcgcgcat
     2341 ccgcgagctc gagcacgcgc tgaccgccta cgagatgatg gggcccgtgc gcgccgagga
     2401 tttcgagcgc gcgctgcggc gctacgtcga gttccaccgc gtccacatga cgctcgagga
     2461 agacgccgtc ctgccgctgg ccgagcgctg gctgaccgac gaggactggc gccacctcga
     2521 ccaggaactc gccggcgagc ccgacccgct caccggccgc cgcgccgacg acgagtaccg
     2581 cctgctgttc gaacgcatcg tggacctcgt cccggcgccg ttcgggttcg gccaggccgc
     2641 gcgctgacgg ctcgccgagc atcagcgggt ctgcgtgctg tgcactgcgg tgcgcgccct
     2701 cttccgggcg cgcgggcgag ggctcacgcc atgggctcga aggcgccggc cacgacctcg
     2761 accaggtgct cgccgggcgg cgtcagctcg acgccgtcgt cgtccatgcg gacgagcccc
     2821 gctcgcacga gcggggccag ctcgcgcaac gcgcgcgcga agcacagccg cggctccacc
     2881 agatgcgcga gcgcgatcgc ctcgaagtcg atccgcccgt ggcacaccag cgaggccacc
     2941 gccgaccggt gcgcgagcgc cgccggcgac agcaccacg
//
LOCUS       DASVLN010000694         2032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_357805, whole genome shotgun sequence.
ACCESSION   DASVLN010000694 DASVLN010000000
VERSION     DASVLN010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357805"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..1078
                     /gene="slmA"
                     /locus_tag="VF453_16400"
     CDS             401..1078
                     /gene="slmA"
                     /locus_tag="VF453_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEX7689295.1"
                     /translation="MTPSTHAESGSGEDVADGSTAAEGPPPEAVAPGRKRPKPGERRV
                     QILQTLAAMLEEPGADRITTAALAAKLAVSEAALYRHFASKAQMFEGLIEFIESSIFT
                     LIHQITEREADPSQQARRIVFMLLQFGEKNPGMARVMVGDALVFENERLTARMNQFFD
                     KVESQLRQCLRAAAERAGSQTPTVDANGRASALTALAVGRLQRYTRSGFKRSPTEQID
                     LALGLLT"
     gene            1134..>2032
                     /locus_tag="VF453_16405"
     CDS             1134..>2032
                     /locus_tag="VF453_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689296.1"
                     /translation="MSEEFKPGTRALLRLAGLDADRSFNSRDDENLALWRAVVVLLMR
                     LYIDPNPESRRTSGAGDAQVVHALRAALAENFGIEAGTHIAWALIHYGCRQKPTMASL
                     QPWERLMFEWQQRRASAPRVALMLRDGGLPDPLEPRALAAVDSWVTDPPSALGEAPGI
                     LQALFGRRLVSFLITEGDGEATHVELFGELLASVVPQGLLDAPRVRSVTDAGGTRWIV
                     EYGFRGAAHAFESPADGPTMRLPGVMNAVDGLLAHIGRPERVFQMLPGRVGPAEPCLF
                     VVADGVRFGELVRRLRLPTLRLPR"
BASE COUNT          278 a          753 c          715 g          286 t
ORIGIN      
        1 tcgatggcgc cggccggcgt gcgcgtcgtg cacagcctgc ggatgctggt ggcgtgggag
       61 ggcggcggcg acgagggcga cgatgcgggc gagctgaacc gccgctgaag ccgcggcgcg
      121 ccttcgcggc gcgcgcgccc gccgcggcgt gacgtccttc tcgcccggat gccgcgcgac
      181 gggattttcg tcggccatcc ccgtgcaaac cccgagcatt tcgacgcccg cggccgaact
      241 ttgcttggtg tttacctggg cgcagcagcg cagcagtggt gtgcaaaaat cgattccaga
      301 ccggccatcc cggcagcact cccggaacct gacagcgatg ccagactcct ccaccgcctt
      361 tcctgccgtt gccgatcctg gcgtttcggc gcctgaacac atgacgcctt ccacgcacgc
      421 agagtccggc tccggggagg acgtcgccga cggatcgacc gcggcggaag gcccgcctcc
      481 cgaggcggtc gcgcccggcc gcaagcgccc gaagccgggc gaacgccggg tgcagatcct
      541 gcagacgctc gccgccatgc tggaggagcc cggcgccgac cgcatcacca ccgccgccct
      601 ggccgccaag ctggccgtga gcgaggccgc gctgtaccgt cacttcgcga gcaaggccca
      661 gatgttcgag ggcctgatcg agttcatcga gtccagcatc ttcacgctga tccaccagat
      721 caccgagcgc gaggccgacc cgtcgcagca ggcgcgtcgc atcgttttca tgctgttgca
      781 attcggcgag aagaaccccg gcatggcgcg cgtgatggtc ggcgacgccc tcgtgttcga
      841 gaacgaacgc ctgaccgccc gcatgaacca gttcttcgac aaggtggagt cgcagctgcg
      901 ccagtgcctg cgcgccgctg cggaacgggc cggctcgcag acgcccaccg tcgacgcgaa
      961 cgggcgtgcc tcggccctca cggcgctcgc cgtcgggcgt ctgcagcgct acacgcgctc
     1021 gggcttcaag cgcagcccga ccgagcagat cgacctcgcc ctcggcctcc tgacctgatc
     1081 ggctgcaagc acgccgtgcc ccgcgcgagc gggcatgacg gcatactgcg aggatgagcg
     1141 aagaattcaa gcccgggacg agagcgctgc tgcgcctcgc gggcctggac gccgaccggt
     1201 cattcaactc gcgcgacgac gagaacctcg cgctgtggcg ggcggtcgtc gtcctgctga
     1261 tgcgcctgta catcgacccg aaccccgagt cgaggcgcac ctccggcgcc ggggacgcgc
     1321 aggtcgtgca cgcgctgcgc gccgcgctgg ccgagaactt cggcatcgag gccggcaccc
     1381 acatcgcctg ggcgctcatc cactacggct gccgccagaa gcccacgatg gcctcgctgc
     1441 agccgtggga gcggctgatg ttcgaatggc agcagcgccg cgccagcgcg ccgcgcgtcg
     1501 cgctgatgct gcgcgacggc ggcctgcccg acccgctgga gccgcgcgcg ctcgccgcgg
     1561 tcgacagctg ggtgacggac ccgccgtcgg cgctcggcga ggcgccgggc atcctgcagg
     1621 cgctgttcgg gcgccggctg gtgagcttcc tcatcaccga gggcgacggc gaggcgaccc
     1681 acgtcgagct gttcggcgag ctgctcgcct cggtggtgcc gcagggcctg ctcgacgcgc
     1741 cgcgcgtgcg ttcggtgacc gacgccggcg gcacgcgctg gatcgtcgag tacggcttcc
     1801 gcggcgccgc ccacgcgttc gagtcgcctg ccgacggccc gacgatgcgc ctgccgggcg
     1861 tcatgaacgc ggtcgacggg ctgctcgcgc acatcggccg gccggagcgc gtgttccaga
     1921 tgctgcccgg gcgcgtcggc ccggcggagc cgtgcctgtt cgtcgtcgcc gacggcgtcc
     1981 gcttcggcga gctggtgcgc cgcctgcgcc tgccgaccct gcgcctgccg cg
//
LOCUS       DASVLN010000695         6461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_89546, whole genome shotgun sequence.
ACCESSION   DASVLN010000695 DASVLN010000000
VERSION     DASVLN010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89546"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VF453_16410"
     CDS             <1..560
                     /locus_tag="VF453_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7689297.1"
                     /translation="EPLADAVRTALSSAIAAAAPPPQMRFDNVDAHVAYLRWLGTMSD
                     RMKKYKTESRDRIEFLQTLWYESHRAGLEPALVLGLVQVESGFRKYAISSAGARGYTQ
                     VMPFWARQIGDGDAARLFLMQVNLRFGCVILRHYLDVEQGDLFMALGRYNGSRGRPEY
                     PDAVLGARRKWLMPAAAPSVALKAN"
     gene            complement(547..1176)
                     /locus_tag="VF453_16415"
     CDS             complement(547..1176)
                     /locus_tag="VF453_16415"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX7689298.1"
                     /translation="MTAAAAGESRRPAVVVGITGASGAAYGVRLLERLGELGAETHLV
                     VTPAGVINAHHELGLARDELAARATHAWPIGDIGACIASGSYGAQAMVVAPCTMKSLA
                     AIAHGFGDNLLTRAADVMLKERRRLVLMVRETPFNLAHLRNMTAVTEMGGIVFPPLPA
                     LYQRPQTIAEMVDETVERVIELLGVPGATPRRWNGLRAGGEPGGPFNSP"
     gene            complement(1173..1487)
                     /gene="grxD"
                     /locus_tag="VF453_16420"
     CDS             complement(1173..1487)
                     /gene="grxD"
                     /locus_tag="VF453_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX7689299.1"
                     /translation="MDDVQKRIDDLVKNNTVVLFMKGTAQFPMCGFSGRAIQILKACN
                     VKELKTFNVLEDEGVRQGIKEYANWPTIPQLYVKGEFVGGSDIMMEMYESGELQPLLA
                     AA"
     gene            complement(1608..2147)
                     /locus_tag="VF453_16425"
     CDS             complement(1608..2147)
                     /locus_tag="VF453_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689300.1"
                     /translation="MEQSHSARPRVRWSLLAAAMAAACTLHGTARASDGATPPTTRPA
                     DSPYQLAPARGLASPVRALGSYYLFDPAQSTLGPTMTSLLGGLRLSTGVVGMGQPMSV
                     LDNPHDSAQNLPYFGLGYSHLWFHSQLSLNADFGLASGSGVGFGRGRGLLGALPSIEE
                     TSGDPRWAPVMAVNVRYSF"
     gene            complement(2452..3303)
                     /gene="prmC"
                     /locus_tag="VF453_16430"
     CDS             complement(2452..3303)
                     /gene="prmC"
                     /locus_tag="VF453_16430"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX7689301.1"
                     /translation="MTAESVAAALREARALGVDRLDAQLLLGQVLGRSRAWLIAHDDA
                     AMAPADAAAFARLAERRAAGEPLAYLLGEREFHGLALAITPAVLVPRPDTETLVDWAL
                     RLLHAPPLAQVAAPAVVDLGTGSGAIALAIKASSRATVWAVERSPQALAVARRNASEL
                     GLDVHFAQGDWWQALEGRLDAPAAFDLVVGNPPYIAPGDPHLAALSHEPIAALVAEDE
                     GLADIARIAQGAPARLRTGGWLLLEHGWEQAPAVAAILARAGLVDVQTVADIEGRPRC
                     TGGRLPR"
     gene            complement(3300..4412)
                     /gene="prfA"
                     /locus_tag="VF453_16435"
     CDS             complement(3300..4412)
                     /gene="prfA"
                     /locus_tag="VF453_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX7689302.1"
                     /translation="MKDSLRQQFDRLGMRLQELDARLADPAVTADVQQLRELSREQSE
                     AAEIVALYRRFTQRESDLAEARGLQADPEMAEMAREEIAACEADLAALEGQLQAALLP
                     RDPDDARPAFVEIRAGTGGEESALFAADLLRMYLRYAERQGWRSEVMSESTSDLGGYK
                     EVVVRLEDPTARRGLAASEGVYGQLRFESGGHRVQRVPATETQGRIHTSACTVAVLAE
                     PDEAAEVTLNPADLRIDTFRASGAGGQHINKTDSAVRITHLPTGVVAECQDDRSQHRN
                     KAKAMAVLLARLRDRERAEREARDAATRRSLVGTGDRSDRIRTYNFPQGRLTDHRINL
                     TLYKLGAVMEGELDDVVAALRAARAQEQLAALESGA"
     gene            complement(4525..5844)
                     /gene="hemA"
                     /locus_tag="VF453_16440"
     CDS             complement(4525..5844)
                     /gene="hemA"
                     /locus_tag="VF453_16440"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX7689303.1"
                     /translation="MSVLALGLNHQTAPVDLRGRFAFPVDQIPPALKALAAQFTPRGA
                     AGAAGAPEVALLSTCNRTELYVAAPSDLPGALVRPAAEWLAAHGGVSGAQLLDHSYVR
                     ENREAARHVFRVASGLDSMVLGEPQILGQMKQAVREADEAGTLGTTLHQLFQRSFSVA
                     KEVRTSTEIGAHSISMAAAAVRQATQLFGELDKLNVLFVGAGEMIELVATHFAARSPK
                     AMVVANRTLERGEKLALRLGAQALRLADMPARLHEFDVVVSCTASTLPIIGLGAVERA
                     LKTRKRRPMFMLDLAVPRDIEPEVAQQRDVYLYTVDDLSELVRTAGEKRQAAVQQAEA
                     IIDAGVLGFSHWLDQRATVPLIQALTRQADDWRTLELQRARKAIAKGESVDVVLESLS
                     RGLTQKMLHGTLAELRSTEGEQRHRLVDTISRLFLRQSARDPSEPGL"
     gene            5959..6231
                     /locus_tag="VF453_16445"
     CDS             5959..6231
                     /locus_tag="VF453_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689304.1"
                     /translation="MDALGLFWHLANFVAPALAVGALSALLCKAFWRRALARTSWFTL
                     AWQSAAAGLAVLVAGLAITGHDGRLATYAALVAACGLVAWTKTIRR"
BASE COUNT          842 a         2272 c         2413 g          934 t
ORIGIN      
        1 aggagccgct cgccgacgcc gtgcgcaccg cgctgagctc ggcgatcgcg gccgccgcgc
       61 cgccgccgca gatgcgcttc gacaacgtcg acgcccacgt cgcctacctg cgctggctcg
      121 gcacgatgag cgaccggatg aagaagtaca agaccgagag ccgcgaccgc atcgaattcc
      181 tgcagacgct ctggtacgag agtcaccgcg cgggactcga gccggcgctg gtgctgggac
      241 tggtccaggt cgagagcggc tttcgcaagt acgcgatcag ctccgccggc gcgcgcggct
      301 acacccaggt gatgccgttc tgggcgcgcc agatcggcga cggcgacgcg gcacggctgt
      361 tcctgatgca ggtcaacctg cgcttcggct gcgtgatcct gcgccactac ctggacgtcg
      421 agcagggcga cctcttcatg gcgctcggtc gctacaacgg cagccgcggg cggcccgagt
      481 atcccgacgc cgtactcggc gcgcgccgca agtggctgat gccggcggct gcgccttcgg
      541 tggcgctcaa ggcgaattga acgggccgcc gggctcgccc ccggctcgga gcccgttcca
      601 gcgccgcggc gtcgcgccgg gcacgccgag cagttcgatc acgcgctcga cggtctcgtc
      661 gaccatctcg gcgatggtct gcggacgctg gtagagcgcc ggcagcggcg ggaagacgat
      721 gccgcccatc tccgtgacgg cggtcatgtt gcgcaggtgc gcgaggttga acggcgtctc
      781 gcgcaccatc agcaccaggc ggcggcgttc cttgagcatc acgtcggccg cgcgcgtgag
      841 caggttgtcg ccgaagccgt gcgcgatcgc cgcgagcgac ttcatcgtgc aaggcgccac
      901 caccatcgcc tgcgcgccgt agctgccgct ggcgatgcag gcgccgatgt cgccgatcgg
      961 ccaggcgtgc gtcgcccgcg cggcgagctc gtcgcgcgcg agcccgagct cgtgatgcgc
     1021 gttgatcacg cccgcagggg tcacgacgag gtgggtttcc gcgccgagct cgccgaggcg
     1081 ttccagcagg cgcacgccgt aggccgcgcc ggacgcgccg gtgatcccga cgacgacggc
     1141 cggacggcgc gattcgcccg cggctgcagc ggtcaagcgg cggccagcag cggctggagt
     1201 tcgcccgact cgtacatctc catcatgatg tccgagccgc cgacgaactc gcccttgacg
     1261 tacagctgcg ggatcgtcgg ccagttggcg tattccttga tgccctggcg cacgccctcg
     1321 tcctcgagga cgttgaaggt cttgagctcc ttcacgttgc aggccttcag gatctggatg
     1381 gcgcggccgg agaagccgca catcgggaac tgggcggttc ccttcatgaa caacacgacg
     1441 gtgttgttct tcacgaggtc atcgatgcgt ttttgaacgt cgtccatggc aatgggaggt
     1501 cgagctggcg cgccgcggcg cggacagtga agtttataag ccaaaccgac gcgccccgca
     1561 ggcgcaaggc gatgggatgt cgactggagc gccgccggga cggacgctca gaacgaatag
     1621 cggacgttga cggccatcac cggcgcccag cgcgggtcgc cgctggtctc ctcgatcgag
     1681 ggcagcgcgc cgagcaggcc gcgcccgcgg ccgaagccca cgccgctgcc cgacgcgagg
     1741 ccgaagtccg cattcaggct cagctggctg tggaaccaga ggtggctgta gccgaggccg
     1801 aagtagggca ggttctgcgc gctgtcgtgc ggattgtcga gcaccgacat cggctggccc
     1861 atgccgacca cacccgtgct gaggcgcaag ccgccgagca ggctggtcat ggtggggccg
     1921 agcgtgctct gcgccgggtc gaagaggtag tagctgccca gcgctcggac cgggctggcg
     1981 aggccgcgcg ccggcgcgag ctggtagggg ctgtcggcgg ggcgcgtcgt gggcggcgtc
     2041 gcaccgtcgc tcgcgcgcgc cgtgccgtgc agcgtgcagg cggcggccat cgcggccgcc
     2101 aggaggctcc agcgcacgcg cgggcgtgcc gaatgcgact gttccatggt gggctcctga
     2161 tcgggtcgac gacgcgagaa accggactgg ggacgagacg atgctacccg tcaagtcgtg
     2221 ctggagatat cggcaatcag aacggatttt cgccgtcgga ttaccggttg tgacattccc
     2281 gacacctggg cacaagccag cgctttctcg tgcttgcgcg atcgccgtgg ccggactaga
     2341 aagcgcgatg gcctcgcatg gcgatcgtcc gcgatccgcc tttcggcatg ctgagcgtgc
     2401 gctgagggta tcggttagcg agcgcctaca tgcgcgctcg ccgcggccgg ctcagcgcgg
     2461 cagacgtccg cccgtgcagc gcggcctgcc ctcgatgtcg gcgaccgtct gcacgtcgac
     2521 gagcccggcg cgcgcgagga tcgccgccac cgccggtgcc tgctcccagc cgtgttccag
     2581 cagcagccat ccgcccgtgc gcaggcgtgc cggggcgccc tgggcgatgc gggcgatgtc
     2641 ggccaggccc tcgtcctcgg ctaccagcgc ggcgatgggc tcgtgcgaga gcgccgccag
     2701 gtgcgggtcg ccgggcgcga tgtagggcgg gttgccgacg acgaggtcga aagcggccgg
     2761 tgcgtcgagg cggccctcca gcgcctgcca ccagtcgccc tgcgcgaagt ggacgtccag
     2821 cccgagctcg ctcgcgttgc gtcgcgcgac ggccagtgcc tgcgggctgc gctccacggc
     2881 ccacacggtg gcgcgcgagc tggccttgat cgccagcgcg atcgcaccgc tgcccgtgcc
     2941 caggtcgacg acagccggtg ccgcgacctg cgccaggggc ggggcgtgca gcagccgcag
     3001 tgcccagtcc accagcgtct cggtgtcggg gcgcggcacc agcaccgcgg gcgtgatggc
     3061 gagcgcgagg ccgtggaact cccgttcgcc gagcaggtag gcgaggggct cgcccgcggc
     3121 gcggcgttcc gccagcctcg cgaaggccgc ggcgtcggcg ggagccatcg ccgcgtcgtc
     3181 gtgcgcgatc agccacgccc gcgagcggcc cagcacctgt ccgagcagca gctgtgcgtc
     3241 gagccggtcc acccccagcg cgcgcgcctc gcgcagcgcc gcggccacgc tttccgccgt
     3301 cacgcgccgg actccagcgc ggccagctgc tcctgcgcgc gcgcggcacg cagcgcggcg
     3361 acgacatcgt cgagctcgcc ctccatcacg gcgcccagct tgtacagcgt caggttgatg
     3421 cggtggtcgg tcagccggcc ctgcgggaag ttgtaggtgc ggatgcggtc ggagcggtcg
     3481 cccgtgccga ccaggctgcg ccgcgtcgcc gcatcgcgcg cctcgcgttc cgcgcgctcg
     3541 cggtcgcgca ggcgcgcgag cagcaccgcc atcgccttgg ccttgttgcg atgctgcgag
     3601 cgatcgtcct ggcactcggc gaccacgccc gtcggcaggt gcgtgatgcg caccgcgctg
     3661 tccgtcttgt tgatgtgctg gccgccggcg ccgctcgcgc ggaaggtgtc gatgcgcagg
     3721 tcggcggggt tgagcgtcac ctccgcggcc tcgtccggct cggcgagcac cgccaccgtg
     3781 cacgcgctgg tgtggatgcg gccctgcgtc tcggtggccg gcacgcgctg cacgcggtgg
     3841 ccgcccgact cgaagcgcag ctggccgtac accccctcgg aggccgcgag cccgcgcctg
     3901 gccgtcggat cctccagccg caccacgacc tccttgtagc cgccgaggtc ggacgtgctc
     3961 tcgctcatca cctcgctgcg ccagccctgg cgctcggcat agcgcaggta catgcgcagc
     4021 aggtcggcgg cgaacagcgc cgactcctcg ccgccggtgc cggcgcggat ctcgacgaag
     4081 gccgggcgcg cgtcgtcggg atcgcgcggc agcagcgcgg cctggagctg gccctcgagc
     4141 gccgccaggt ccgcctcgca ggcggcgatc tcctcgcgcg ccatctcggc catctcggga
     4201 tcggcctgca gcccgcgcgc ttcggccagg tcggactcgc gctgcgtgaa gcggcggtac
     4261 agcgcgacga tctccgccgc ctcggactgc tcgcgcgaga gctcgcgcag ctgctgcacg
     4321 tccgccgtga ccgccggatc cgcgagccgc gcgtcgagct cctgcagccg catgccgagg
     4381 cggtcgaact gttggcggag ggaatctttc attgcgcggg aggggcgggc gacgcggtcg
     4441 tcggggatct gcggcggagc gcgcgacggg gttggcgcgc ggggaaggcg tgcgcggacg
     4501 gcacgcggcg gcgatgaggc gccgctacag gcccggctcg gacgggtcgc gcgccgactg
     4561 gcgcaggaac aggcgcgaga tcgtgtcgac caggcggtgg cgctgctcgc cctcggtcga
     4621 ccgcagctcg gcgagggtgc cgtgcagcat cttctgcgtc aggccgcggc tcagcgactc
     4681 gagcacgacg tcgacggatt cgcccttggc gatcgccttg cgcgcgcgct gcagctcgag
     4741 cgtccgccag tcgtcggcct ggcgggtcag cgcctggatc agcggcacgg tggcgcgctg
     4801 gtcgagccag tgggagaagc ccagcacgcc ggcgtcgatg atggcctcgg cctgctgcac
     4861 ggcggcctgg cgcttctcgc cggccgtgcg cacgagctcg gacaggtcgt cgacggtgta
     4921 gaggtagacg tcgcgctgct gggcgacctc gggctcgatg tcgcgcggca cggcgaggtc
     4981 gagcatgaac atcgggcggc gcttgcgggt cttgagggcg cgctcgacgg cgcccagccc
     5041 gatgatcggc agcgtgctgg cggtgcagga gacgacgacg tcgaactcgt gcagccgcgc
     5101 gggcatgtcg gccaggcgca gcgcctgcgc gcccagccgc agggcgagct tctcgccgcg
     5161 ctcgagcgtg cggttggcca ccaccatcgc cttcgggctg cgggccgcga aatgggtggc
     5221 gacgagctcg atcatctcgc ccgcgccgac gaacaggacg ttgagcttgt cgagctcgcc
     5281 gaacagctgc gtcgcctggc gcacggcggc ggcggccatg ctgatcgagt gggcgccgat
     5341 ctccgtggag gtccgcacct ccttggccac cgagaacgag cgctggaaca gctggtgcag
     5401 cgtggtgccg agcgtgccgg cctcgtcggc ctcgcgcacc gcctgcttca tctggccgag
     5461 gatctgcggc tcgcccagca ccatcgagtc gaggccggag gcgacgcgga agacgtggcg
     5521 cgcggcctcc cggttctcgc ggacgtagct gtggtcgagc agctgcgcgc cgctgacgcc
     5581 gccgtgcgcg gccagccact cggcggcggg gcgcacgagc gcgccgggca agtcggaagg
     5641 cgcggcgacg tagagctcgg tgcggttgca ggtcgacaac agcgccacct cgggcgcgcc
     5701 ggcggcaccg gctgcgccgc gcggggtgaa ctgggcggcg agcgccttca gggccggcgg
     5761 gatctggtcg accgggaacg cgaaccggcc gcgcaggtcg accggcgcgg tctggtggtt
     5821 gagtcccaac gcgagcacag acatcccggc attataaaat ccgcgggccc cgggcgcttg
     5881 cgcacggtca tgccgccagc agccggcgtg cccggaatgc ccgcttttcg cgccgtcgcg
     5941 ctgactcacc gccgcccctt ggacgccctc ggcctgttct ggcacctcgc caacttcgtc
     6001 gctcccgcgc tggcggtggg cgcactttcc gcgctcttgt gcaaggcgtt ctggcgccgc
     6061 gcgctggcgc gcacgtcgtg gttcacgctc gcctggcagt cggccgcggc cgggctggcg
     6121 gtgctggtcg cggggctcgc catcaccggg cacgacgggc ggctcgccac ctacgcggcg
     6181 ctggtcgcgg cctgcgggct ggtcgcctgg acgaagacga tccgccgctg aagcgcccat
     6241 gaacgggaac ggccgcggcg cgggtgcgcg gcggccgtgg cggggcgcgc gaccggtcgg
     6301 cggtcgcgcc ggctacgcgg tcagaaccag atctcgacct gggcgccgta ggacacggcg
     6361 ttggtcttgt ccggacggaa gccgtagttg ttgttggtgt cctgcgcggc ggccttgttg
     6421 tagtcggcgt aggtcacgta caggcgcagc tcggggcgct t
//
LOCUS       DASVLN010000696         6308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_357942, whole genome shotgun sequence.
ACCESSION   DASVLN010000696 DASVLN010000000
VERSION     DASVLN010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357942"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1934)
                     /locus_tag="VF453_16450"
     CDS             complement(<1..1934)
                     /locus_tag="VF453_16450"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7689305.1"
                     /translation="MAPRLSPWQVLGLSHEASVADVRRAYAALIKQYRPDSHPEEFAR
                     IREAYETALPAARRREAQIEAQRDAARQAAPEHGFARADENDGAEPDDGTLTRDESPA
                     GGEDVLHAPPQREPGREPLIADPAQVDTARPALSPPRPPVRVNTDAPVLDARPVDPLE
                     SPVRIDTASPSFDAAPPAPVRIAIEPPVLDAPPHPDPARVDTSHPDLAPDRSPPPSAP
                     PVRVLGSSPFMSPSPQPKPVRIEARPPEASAPPRPPAPKPPRVRPRPQPAEAATRGEP
                     ALAERVAVVRKRRSDAGEDAALPWLRTLLRRSNRASIDDRQALEIALLEWFLQDEDPP
                     LLLMFDAGRAFGWHRQESRLAPWLPAGADRRLELLLTMSRDLVFARHGSRGTVRRLFT
                     PGMPVPPFAFTIAQIEARGWEQRWLALQAAAGAPALQSRLNQRARRALGGGVLLSTDL
                     FLGALVALACSGAAVDLLVDATGSPSLHRIPDWLATCVTAVLVAIVIALLRRAAVHGL
                     RLFAQSPRLAPLRRLVARARAQRGVSVGVVLLSIFLVAVIADAFRSEMLLWAGFGLLA
                     GVFAVIVLGWLLRFAWLLAFQVERVAHGLTAWRESVDRWEFERLLAQPIQVGAQADGS
                     VPRRPPPPFGARAGLRARLMA"
     gene            complement(1934..3631)
                     /locus_tag="VF453_16455"
     CDS             complement(1934..3631)
                     /locus_tag="VF453_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX7689306.1"
                     /translation="MIFGIDLGTTNSLIAYLKDGVPTAIPNVHGSVLTPSVVSVDADG
                     SLMVGATARERLVTQPKRSVGSFKRLMGTAQAVTLAGRVFKPEDLSALVLRSLKADAE
                     QATGETVQDVVISVPAYFNDSQRKATRVAGELAGLNVRRLVNEPTAAALFHGIADRDG
                     DSTFLIVDLGGGTFDVSILERFSGVMEVRATGGDSFLGGDDFTTAMVQLLARRAGLND
                     SQRDAGAVAAALHYAAESAKRALGTHANCEVDLQAVGLGVIPLALHEYEQACEPLIDR
                     LRAPIERALRDARLRAAELDEIALVGGASRMPMVRRLVSQLFGRLPARHLDPDQTIAL
                     GAAVCAGLVGRDAAFEEMVLTDVCPHTLGTRVSESDGRGGYDTDVFLPIIERNTVVPA
                     SRMKSVVTLRDNQTTMTIDIYQGESRHCSQNIPLGSIDLTLPALPAGQVQADVRYTYD
                     VNGILDVDVSVNKHNIAKNLVVRKLAGDVSDEEIERRRAELAALKVHPRDQDENRLLL
                     ERANRLYEQLLGGERERLGQMIGQFDATLRGQEPHEIATARRAFGSALDSLERGPTW"
     gene            complement(3892..4119)
                     /locus_tag="VF453_16460"
     CDS             complement(3892..4119)
                     /locus_tag="VF453_16460"
                     /inference="COORDINATES: protein motif:HMM:NF044532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689307.1"
                     /translation="MPEVSAKAIAVSIRAVQAEVDRLQEELKTADPEDIPDTQELLLS
                     VSQAAIELKKAYEHALRMSNNLVPYEELIAR"
     gene            complement(4134..4661)
                     /locus_tag="VF453_16465"
     CDS             complement(4134..4661)
                     /locus_tag="VF453_16465"
                     /inference="COORDINATES: protein motif:HMM:NF037873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689308.1"
                     /translation="MYFYGRFPRRQHGCSRLATRDSSWLLSRSPSSARFARKSFPNGT
                     IISDKPADGVLHPSFVKTLITGGKRKGQWRDPDVQTFTSGDVLYAQTGGGTSLFDKKG
                     VFGDAFWHSFEIPAGTEIPDSLKMEGPDWNPVYKANHYQIECKAKTMRVDSMKGALDN
                     LARNAIVRKIALASH"
     gene            complement(5000..6031)
                     /locus_tag="VF453_16470"
     CDS             complement(5000..6031)
                     /locus_tag="VF453_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7689309.1"
                     /translation="MRTLLEPRLVDPGGREPGLVVEVRDHRRALLFDLGELGPVSART
                     LLRASHAFVTHAHMDHFGGFDHWLATSLGRKPSMTLWGGPGFVDRVEHKLRAYVWNVV
                     QRYPVPLVVEVRALEADGTRRGAVFDSRDGFLRRDLVIAADAGDVLVEEPLLRVRARI
                     VDHEMPVLAFAIEETAQVRVAADRIAAMGLATGPWLQTLKAAVLADAPDGQPMAVRWR
                     DAAGEHERRFTLGELRPLVLDRVPGRRLGYVTDLRFTEANLRQLDALLRDVDLLHIEC
                     VFSEDDRALARQRNHLTARQAGTVARRLGARRLVPFHFSPRYAGREDVLREQAFAAWR
                     GETAADVAE"
BASE COUNT          950 a         2134 c         2296 g          928 t
ORIGIN      
        1 gccatcaggc gggcccggag gccggcgcgc gcgccgaacg gcggcggggg ccggcgcggc
       61 accgagccat cggcttgcgc gccgacctgg atcggttgcg cgagcaggcg ctcgaactcc
      121 cagcggtcga cggactcgcg ccacgcggtc aggccgtggg cgacgcgctc gacctggaaa
      181 gccaggagcc acgcgaagcg cagcagccag cccagcacga tcaccgcgaa cacgccggcg
      241 aggaggccga atccggccca gagcaacatc tcgcttcgga atgcgtcggc gatcaccgcc
      301 acgaggaata tcgagagcaa gaccacgccg acgctgacgc cacgctgcgc gcgcgcccgg
      361 gccaccaggc gccgcagcgg ggccagccgc ggcgactgtg cgaagaggcg caggccatgt
      421 accgccgcgc ggcgcagcag agcgatcacg atcgccacca gcactgctgt cacgcaggtg
      481 gccagccagt ccgggatgcg gtgcagggac ggcgagccgg tcgcgtcgac caggaggtcc
      541 accgccgccc cgctgcaggc cagcgcgacg agtgcgccga ggaacaagtc ggtcgacagc
      601 agcacgccac ccccgagtgc gcgccgggca cgctggttca ggcgcgactg cagcgcaggg
      661 gcgccggccg ctgcctgcaa tgccagccat cgctgctccc agccgcgcgc ttcgatctgc
      721 gcgatcgtga acgcgaacgg cggcacgggc atgccgggcg tgaagagacg gcgcacggtg
      781 ccgcgacttc catggcgcgc gaagaccagg tcgcgcgaca tcgtcagcag cagctcgagc
      841 cgccggtccg cgcccgccgg cagccagggc gcgaggcgcg attcctggcg atgccagccg
      901 aacgcacggc cggcgtcgaa catcagcagc agcggcggat cttcgtcctg caggaaccat
      961 tcgaggagcg cgatctccag cgcctgccga tcgtcgatcg acgcgcggtt gctccggcgc
     1021 agcagggtgc gcagccacgg cagcgcggcg tcctcgccgg catcgctgcg gcgcttgcgc
     1081 acgaccgcca cgcgttcggc aagcgccggc tcgccacgcg tcgcagcttc ggccggctgc
     1141 gggcgcggac ggacgcgagg aggcttcggt gccggcggcc gcggaggagc ggatgcttcc
     1201 ggcgggcgtg cctcgatgcg caccggcttc ggctgcggcg acggcgacat gaatggcgac
     1261 gagccgagca cccgcaccgg cggggcgctt ggcggcggcg atcggtccgg cgcgagatcc
     1321 gggtgcgacg tgtcgacccg tgcaggatcc gggtggggcg gcgcgtcgag caccggtggt
     1381 tcgatggcta tgcgcaccgg cgcgggcggc gccgcgtcga acgacggcga ggcggtgtcg
     1441 atgcgcacgg gcgattcgag cggatcgacg ggccgcgcat cgaggacggg ggcgtcggtg
     1501 ttcacgcgga cgggcggacg gggcggcgag agcgcggggc gcgcagtgtc gacctgcgcc
     1561 ggatcggcga tcagcggctc gcggccgggt tcgcgctgtg gcggggcgtg caagacatct
     1621 tcgccgccgg ccgggctctc gtcgcgggtg agcgttccat cgtccggctc ggcaccgtcg
     1681 ttctcgtcgg cccgggcgaa gccgtgctcg ggcgcggcct ggcgcgcggc gtcccgctgc
     1741 gcctcgatct gcgcctcgcg ccgccgcgcc gccggcagcg cggtctcgta ggcttcgcga
     1801 atgcgcgcga attcttcggg atgcgagtcg gggcggtatt gcttgatcag tgccgcgtag
     1861 gcacggcgca cgtcggccac ggacgcttcg tgcgacagcc ccagcacctg ccagggcgac
     1921 agccgcggcg ccatcaccag gtcgggccgc gctcgagcga atcgagcgcg ctgccgaagg
     1981 cacggcgcgc cgtcgcgatc tcgtgcggct cctggccgcg cagcgtcgcg tcgaactgcc
     2041 cgatcatctg gccgaggcgc tcccgttcgc cgccgagcaa ctgctcgtag aggcgattcg
     2101 cgcgctcgag caggaggcga ttctcgtcct gatcgcgcgg gtggaccttc agggccgcga
     2161 gctcggcgcg gcggcgctcg atctcctcgt cgctgacgtc gcccgcgagc tttcgcacga
     2221 cgaggttctt cgcgatgttg tgcttgttca ccgagacgtc gacgtcgagg atgccgttga
     2281 cgtcgtaggt gtagcgcacg tcggcctgga cctggccggc cggcagcgcc ggcagggtga
     2341 gatcgatcga gcctagcggg atgttctggc tgcagtggcg cgactcgccc tgatagatgt
     2401 cgatcgtcat cgtcgtctgg ttgtcgcgca gcgtcacgac gctcttcatg cggctcgccg
     2461 gcacgaccgt gttgcgttcg atgatcggca ggaagacgtc cgtgtcgtag ccgccgcggc
     2521 cgtcggactc ggagacgcgc gtgcccagcg tgtgcgggca gacgtcggtc agcaccatct
     2581 cctcgaacgc ggcgtcacgg cccaccaggc cggcgcagac ggccgcgccg agcgcgatgg
     2641 tctggtcggg gtcgaggtgg cgcgcgggca gccggccgaa caactgcgag acgaggcgtc
     2701 gcaccatcgg catgcgactc gcgccgccga ccagcgcgat ctcgtcgagc tcggcggcgc
     2761 gcaggcgcgc gtcgcgcagc gcgcgttcga tcggtgcgcg cagccggtcg atcagcggct
     2821 cgcaggcctg ctcgtactcg tgcagcgcga gcgggatcac gccaaggccg acggcctgga
     2881 ggtcgacttc gcagttcgcg tgcgtgccga gcgcacgctt ggccgactcg gccgcgtagt
     2941 gcagcgcagc ggccacggcg ccggcgtcgc gctggctgtc gttcagtcct gcgcggcgcg
     3001 cgagcagctg caccatcgcg gtggtgaagt cgtcgccgcc gaggaagctg tcgccaccgg
     3061 tcgcgcgcac ttccatgacg ccgctgaagc gctcgaggat cgacacgtcg aaggtgccgc
     3121 cgccgaggtc gacgatgagg aaggtgctgt cgccgtcgcg atcggcgatg ccatggaaca
     3181 gcgccgcggc cgtcggctcg ttgacgaggc gccgcacgtt gagaccggcc agttccccgg
     3241 cgacacgcgt ggccttgcgt tgcgagtcgt tgaagtaggc cggcacggag atgacgacgt
     3301 cctgcaccgt ctcgccggtc gcctgctcgg catcggcttt cagcgagcgc agcacgagcg
     3361 ccgagagatc ttccggcttg aacacgcggc cggcgagcgt cacggcctgc gcggtgccca
     3421 tcaggcgctt gaacgagccg acgctgcgct tgggctgcgt cacgagccgc tcacgggcgg
     3481 tcgcgccgac catcaggctg ccgtccgcgt cgaccgacac caccgagggc gtcagcacgc
     3541 tgccgtgcac gttgggaatc gcggtgggga cgccatcctt caggtaggcg ataaggctgt
     3601 tggtcgtgcc gagatcgatg ccgaagatca ctgtcccctc ccaggaacgt cgctgttgtc
     3661 agggcggcgc cggcgtggcg cgcgggcctg cggttttgcg ggcgattcta tttctggggc
     3721 cgggcgcgct ccgccggtga gcggacggag tccgacaaag tcgtcacgga accaacggct
     3781 ttcggcgcgg gcacggcggc gggtaacaca gctgctcacc caggcaaact ctcacaagtc
     3841 tccgagctca gttcaataaa tcggtgttgc agttcggatt tgaaaccgcc gctaccgcgc
     3901 aatcaattcc tcgtatggca caagattgtt gctcatgcgt aaggcgtgct cataggcctt
     3961 cttcagctct atggctgcct gcgagactga caggagcagt tcctgagtat ctggaatatc
     4021 ttccgggtcc gcggttttca attcttcttg caacctgtcg acttcggcct gaacggcccg
     4081 aatcgagact gcgatggctt tggcgctgac ttctggcatg aatctttgat cctttagtga
     4141 ctcgcaagcg caatcttgcg cacgatggcg ttccttgcaa ggttgtccag cgcgcccttc
     4201 atcgaatcaa cgcgcatggt cttcgctttg cattcgatct gatagtgatt ggccttgtac
     4261 actggattcc aatccggccc ctccatcttc aaagaatctg gaatctccgt tcctgccggg
     4321 atttcgaaag agtgccagaa cgcgtcaccg aaaacaccct tcttgtcgaa cagcgaggtg
     4381 ccgccgccag tctgagcgta aaggacgtcg cccgaagtaa aggtctggac atcggggtca
     4441 cgccattggc ccttgcgctt gccgccggtg atcagcgtct tcacgaagga gggatgaagc
     4501 acgccatccg caggcttgtc gctgatgata gtgccgttcg ggaaactctt ccttgcgaac
     4561 cgcgcggaag acggtgacct ggacagtagc catgaagaat ccctggttgc tagacgcgag
     4621 cagccgtgct gccgccgcgg gaatctgcca taaaaataca tccgcgggta ccccacgggg
     4681 aggggcttgg aatgcgtagt caggcgctct tcccatccgt gccgcgatcg cacggatcga
     4741 gtgcccgagc accaacgcgc gcgaggtctc gtcccgctca gccatcgtca gggacagcct
     4801 cgatcgactt cgttccgatg gctgtacgcc gcccgtcctc gacaggatgt gtggatcggg
     4861 gtgtgcggcc ggtcaaacag ctggccgatc cgatgcagag cccagccagc cttccttccg
     4921 acgcttctac gtctcggcct gctgaggctc agagtagtag atccgcggtc gctgcttcat
     4981 gtgcacccgg gctctggcgt cactcggcca cgtcggccgc ggtctcgccg cgccaggcgg
     5041 cgaaggcctg ctcgcgcagg acgtcctcgc ggccggcata gcgcggcgag aagtggaacg
     5101 gcacgaggcg ccgcgcgccg agccggcggg cgacggtgcc ggcctggcgc gcggtgaggt
     5161 ggttgcgctg gcgggcgagc gcgcggtcgt cctcggagaa cacgcactcg atgtgcagca
     5221 ggtcgacgtc gcgcagcagc gcgtcgagct ggcgcaggtt ggcctcggtg aagcgcaggt
     5281 cggtgacgta gccgagccgt cgacccggga cgcgatcgag caccagcgga cgcagctcgc
     5341 cgagcgtgaa gcggcgctcg tgctcgccgg cggcgtcgcg ccagcgcacg gccatcggct
     5401 ggccgtcggg cgcgtcggcc agcaccgccg ccttgagcgt ctgcagccac ggcccggtcg
     5461 cgaggcccat cgccgcgatg cggtcggcgg ccacgcgcac ctgcgccgtc tcctcgatcg
     5521 cgaacgcgag cacgggcatc tcgtggtcga cgatgcgggc gcgcacgcgc agcagcggct
     5581 cctcgaccag cacgtcgccg gcgtcggcgg cgatcacgag gtcccggcgc aggaagccgt
     5641 cgcggctgtc gaagacggcg ccgcggcgcg tgccgtcggc ctccaacgcg cgcacctcga
     5701 cgacgagcgg taccgggtag cgctgcacga cgttccacac gtacgcgcgc agcttgtgct
     5761 cgacccggtc gacgaagccg gggccgcccc agagcgtcat cgacggcttg cggccgaggc
     5821 tggtcgcgag ccagtggtcg aagccgccga aatggtccat gtgcgcgtgc gtgacgaacg
     5881 cgtgcgacgc gcgcagcagc gtgcgggcgg acaccgggcc gagctcgccg aggtcgaaca
     5941 gcagcgcgcg ccggtggtcg cggacctcga ccacgaggcc gggctcgcgg ccgccggggt
     6001 cgacgaggcg gggctcgagc agggtgcgca tcggacgatc atgccagcgg cgcgagcgca
     6061 ccggctgccg gcgcgaggaa tagaccgcgt cgcgcgctct gcgctataac gccggcggag
     6121 cgtccgccct ggacgccttc acgaagggga gagaagacga tgaacaggac gatggtgacg
     6181 ggggccgcgg cgttcgcgct ggtactggcc ctggcgctgg ccggctgcgg cggcgactcg
     6241 ggcgcggacg acgccgccgc gctcgcgcag caggcggcga ccgagaaggc gaacgccgag
     6301 gcgctggc
//
LOCUS       DASVLN010000697         3877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_305621, whole genome shotgun sequence.
ACCESSION   DASVLN010000697 DASVLN010000000
VERSION     DASVLN010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305621"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VF453_16475"
     CDS             complement(<1..757)
                     /locus_tag="VF453_16475"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEX7689310.1"
                     /translation="MAESSSTSDLARRARLTFAEQLAGASPRLLDAILNAARILLDKP
                     AERSVAQQRRDNVQALMQRGEEWVRVVNEMLYEAAEMREKPSTISGELVASMQGAMTL
                     QLVDDETVQRDIYISRLGQAIADEAVWELNDLGTRIAAIEGNAELLDHDLFRPPTIAK
                     IYVRSFDAAGLPAECWAGVENAMKYELSLLVTEAYHDANSFLVGRGVMPEVNLRQLIR
                     RSVDSRVTMVASQPMPLDWNHSGGGATGFGPTAY"
     gene            complement(799..1833)
                     /gene="secF"
                     /locus_tag="VF453_16480"
     CDS             complement(799..1833)
                     /gene="secF"
                     /locus_tag="VF453_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7689311.1"
                     /translation="MEFFRIRRDIPFMRHALVLNIISALTFLAAVFFLFTRGLNLSIE
                     FTGGTAVEASYQQTADLARVRATVGALGYGDVQVQNYGTSRDVIIRIPAKGKVDQRAV
                     AGNVFAALCKAESGTLSTRTETTAKGEVVTRPSCAAAGAEPVVLKSSNFIGPSVGEEL
                     ARNGAYALICVAVGIMAYLAVRFEWKFSLAAVVANLHDVVIILGFFAFFQWEFSLSVL
                     AGVLAVLGYSVNESVVIFDRIRETFRRFRKLDTEQVIDHAITSTISRTIITHFCTQMM
                     VLSMLIFGGPTLHYFAMALTIGICFGIYSSVFVAAAIAMWLGVKREDLIKASSKPQSD
                     PNDPNAGAVV"
     gene            complement(1874..3817)
                     /gene="secD"
                     /locus_tag="VF453_16485"
     CDS             complement(1874..3817)
                     /gene="secD"
                     /locus_tag="VF453_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7689312.1"
                     /translation="MNRYPVWKYVVLVVAVLLGALYTVPNFYGDAPAVQVSAGKTAVH
                     VDDGTVAQVKALLAGAKIEPDYVTLDNGTIRARFRDTDTQYRAKDVLANGLNAGGDPE
                     SPAYVVALNLQSRAPAWMAAVRAAPMYLGLDLRGGVHFLLQVDLSAAVDKKVDTLVTD
                     IRTRLREKTLRGAVTRSQNVVSVRFHDAAAAAKAQDLLNDDFSDWQWTQSTDGADTVF
                     TGTIKPPALQKIQDAAIKQNMTTLGNRVAGLGTTEPVIQQQGPDRIVVELPGIQDTAK
                     AKETIGRTATLEVHMVDESAEGQAAIGNPSATLPAGDDRYLDRNHISVPVKKDIVISG
                     DDLVDAQPNFDQQSQSAIVNLTVNSRGAKILSDITRDNVGKRMAIVLFEKKTGEIITW
                     PRIQSELGERFQITGMANIQEANDLALLLRSGSLAAPMDIVEESAIGPSLGADNIAKG
                     LHSVAWGFVAIMAFMCLYYLLFGVFSTLSLAVNLLMLVAVLCWIHTTLTLPGIAAIAL
                     TLGMAIDANVLVNERIREELRAGSAPQTAIAAGYERAWATILDSNVTTLIAGVSLLAF
                     GSGPVRGFAIVHCLGILTSMFSAVFFSRGLVNLWYGRQKKLKSVAIGQVWKPGEASAE
                     LPRPDDDVPAARAGAKKRPGVAR"
BASE COUNT          586 a         1303 c         1368 g          620 t
ORIGIN      
        1 cgtaggccgt cgggccgaag ccggtcgcgc cgccgccgct gtggttccag tccagcggca
       61 tcggctgcga cgccaccatc gtcacccgcg agtcgacgct gcgccggatc aactggcgca
      121 ggttgacctc gggcatcacg ccgcggccga cgaggaagct gttcgcgtcg tggtacgcct
      181 ccgtgacgag cagcgacagc tcgtacttca ttgcgttctc gacgccggcc cagcactcgg
      241 ccggcaggcc cgccgcgtcg aacgagcgca cgtagatctt ggcgatcgtg ggaggccgga
      301 acaggtcgtg atcgagcagc tccgcattcc cttcgatcgc cgcgatgcgc gtgccgaggt
      361 cgttcagctc ccagaccgcc tcgtcggcga tcgcctggcc caggcgcgag atgtagatgt
      421 cgcgctgcac cgtctcgtcg tcgaccagct gcagcgtcat cgcgccctgc atcgaggcga
      481 ccagctcgcc cgagatcgtc gagggcttct cccgcatctc ggcggcctcg tacagcatct
      541 cgttgacgac gcgcacccac tcctcgccgc gctgcatcag ggcctggacg ttgtcgcggc
      601 gctgctgcgc gacgctgcgc tcggccggct tgtccaggag gatgcgggcc gcgttgagga
      661 tcgcgtccag caggcgcggg ctcgccccgg ccagctgctc ggcgaacgtc agccgggcgc
      721 ggcgcgcaag atcggaagtc gacgaacttt cagccatcgg gcccgcttcg cttgtcggtg
      781 gtgcgattcc cgtcgaatct acaccaccgc gccggcgttc gggtcgttcg gatcggattg
      841 cggcttggac gaggccttga tcaggtcctc gcgcttgacg cccaaccaca tcgcgatcgc
      901 cgccgcgacg aacaccgacg aatagatgcc gaagcagatg ccgatggtca gcgccatcgc
      961 gaagtagtgc agcgtcgggc cgccgaagat cagcatcgac agcaccatca tctgcgtgca
     1021 gaagtgggtg atgatcgtgc ggctgatggt gctcgtgatc gcatggtcga tcacctgctc
     1081 ggtgtcgagc ttgcggaacc gccggaacgt ctcgcggatg cggtcgaaga tgacgacgga
     1141 ctcgttgacc gagtagccca gcaccgccag cacgcccgca agcaccgaca gcgagaactc
     1201 ccactggaag aacgcgaaga agcccaggat gatgacgacg tcgtgcaggt tggcgacgac
     1261 ggcggccagc gagaacttcc actcgaagcg caccgccaag taggccatga tgcccacggc
     1321 cacgcagatc agcgcgtagg cgccgttgcg ggcgagctcc tcgccgaccg acgggccgat
     1381 gaagttgctg ctcttgagga cgaccggctc cgcaccggcc gcggcgcagg acggccgcgt
     1441 gacgacctcg cccttggccg tggtctcggt ccgggtcgag agggtgcccg actccgcctt
     1501 gcacagcgcg gcgaacacgt tgcccgcgac cgcgcgctgg tcgaccttgc ccttggccgg
     1561 gatgcggatg atgacgtccc gcgaagtgcc gtagttctgc acctgcacgt cgccgtagcc
     1621 gagcgccccg acggtggcgc gcacgcgcgc gaggtcggcg gtctgctggt aggaggcctc
     1681 gaccgccgtg ccgccggtga actcgatcga caggttcagc ccgcgcgtga agaggaagaa
     1741 caccgcggcg aggaaggtca gcgccgagat gatgttgagc accagcgcat gccgcatgaa
     1801 cgggatgtcg cgccggatcc ggaagaattc catgagcgtt ccttgagatc gaaggacggg
     1861 ccgggccgcg cgctcagcgc gcgacgcccg ggcgcttctt ggcgccggcg cgcgcggcag
     1921 gcacgtcgtc gtcgggccga ggcagttccg cgctggcctc gcccggcttc cacacctggc
     1981 cgatcgccac cgacttcagc ttcttctggc ggccgtacca gaggttgacc aggccgcgcg
     2041 agaagaacac cgccgagaac atcgaggtca ggatgcccag gcagtggacg atcgcgaagc
     2101 cgcgcaccgg gcccgagccg aaggcgagca gcgacacgcc ggcgatcagc gtcgtgacgt
     2161 tggagtccag gatcgtcgcc cacgcgcgct cgtagccggc ggcgatggcg gtctgcggcg
     2221 cgctgccggc gcgcaattcc tcgcggatgc gctcgttgac cagcacgttg gcgtcgatgg
     2281 ccatgcccag cgtcagcgcg atggcggcga tgccgggcag cgtcagcgtc gtgtggatcc
     2341 agcacagcac cgccaccagc atcagcaggt tgaccgccag cgacagcgtc gagaacacgc
     2401 cgaacagcag gtagtacagg cacatgaagg ccatgatcgc cacgaagccc caggcgacgc
     2461 tgtgcaggcc cttggcgatg ttgtcggcgc ccaggctggg gccgatcgcg ctttcctcga
     2521 cgatgtccat cggcgcggcg agcgagccgg agcgcagcag cagcgcgagg tcgttggcct
     2581 cctggatgtt ggccatgccg gtgatctgga agcgctcgcc gagctcgctc tggatgcgcg
     2641 gccaggtgat gatctcgccg gtcttcttct cgaacagcac gatcgccatg cgcttgccga
     2701 cgttgtcgcg cgtgatgtcc gacaggatct tggcaccgcg cgagttgacc gtcaggttga
     2761 cgatcgcgct ctgcgactgc tggtcgaagt tgggctgcgc gtcgaccagg tcgtcgccgc
     2821 tgatgacgat gtccttcttg accggcaccg agatgtggtt gcggtcgagg tagcgatcgt
     2881 cgccggccgg cagcgtcgcc gacgggttgc cgatcgcggc ctggccctcg gcgctctcgt
     2941 cgaccatgtg gacttccagc gtcgcggtgc ggccgatcgt ctccttggcc ttggcggtgt
     3001 cctggatccc gggcagctcg acgacgatgc ggtcggggcc ctgctgctgg atcaccggct
     3061 ccgtggtgcc caggccggcg acgcggttgc ccagcgtcgt catgttctgc ttgatcgcgg
     3121 cgtcctggat cttctgcagc gccggcggct tgatggtgcc cgtgaagacg gtgtccgcgc
     3181 cgtcggtcga ctgcgtccac tgccagtcgg agaagtcgtc gttgagcagg tcctgcgcct
     3241 tggctgccgc ggcggcgtcg tggaagcgca cggagaccac gttctgcgag cgcgtcacgg
     3301 cgccgcgcag ggtcttctcg cgcagccggg tgcggatgtc ggtgaccagc gtgtcgacct
     3361 tcttgtcgac cgcggccgag aggtcgacct gcagcaggaa gtgcacgccg ccgcgcaggt
     3421 ccaggccgag gtacatcggc gccgcgcgca cggccgccat ccacgccggc gcgcgcgact
     3481 gcaggttcag cgcgacgacg taggcgggcg actccgggtc gccgccggcg ttgaggccgt
     3541 tggcgaggac gtccttggcg cggtactgcg tgtccgtgtc gcggaaacgc gcgcggatgg
     3601 tgccgttgtc gagcgtcacg tagtcgggct cgatcttcgc gccggcgagc agcgccttga
     3661 cctgggcgac cgtgccgtcg tcgacgtgca cggcggtctt gccggccgag acctgcaccg
     3721 ccggggcgtc gccgtagaag ttgggcacgg tgtagagcgc gcccagcagc acggcgacca
     3781 ccagcaccac gtatttccag acaggatagc gattcatgcg ttgttcctcg gggagcggcg
     3841 cgggcccggg cgggcgctcg cgccgcgcgg ccgtcag
//
LOCUS       DASVLN010000698         2953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_89628, whole genome shotgun sequence.
ACCESSION   DASVLN010000698 DASVLN010000000
VERSION     DASVLN010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89628"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..2348)
                     /gene="clpA"
                     /locus_tag="VF453_16490"
     CDS             complement(39..2348)
                     /gene="clpA"
                     /locus_tag="VF453_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX7689313.1"
                     /translation="MIAQELEVSLHMAFVEARQQRHEFITVEHLLLALLDNPSAAEVL
                     RACAANIEDLRKSLSTFIRENTPTVGGSEEVDTQPTLGFQRVIQRAIMHVQSTGGGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNYIAHGIKKSDPPEPPKSAGEGGG
                     EAAEKDEGGEGKGSPLEQFTQNLNQMARDGKIDPLIGREHEVERVIQILCRRRKNNPL
                     LVGEAGVGKTAIAEGLAWRITQNDVPEVLADAVVYSLDMGALLAGTKYRGDFEQRLKG
                     VLKQLKDQPRAVLFIDEIHTLIGAGAASGGTLDASNLLKPALSNGTMKCIGATTFTEY
                     RGIFEKDAALSRRFQKVDVNEPTIEQTVEILKGLKSRFEEHHSVKFALGALQAAAELS
                     AKYINDRHLPDKAIDVIDEAGAAQRILPKSKQKKTITRNEVEEIVAKIARIPPASVST
                     DDRDRLRTIDRDLKSVVFGQDPAIDALSAAIKMARSGLGKPDKPIGAFLFSGPTGVGK
                     TEVAKQLAFILGIDLIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEQVTKKPHC
                     VLLLDEIEKAHPDVFNVLLQVMDHGTLTDNNGRKADFRNVIIIMTTNAGAETMNKSTI
                     GFTTKREQGDEMADIKRLFTPEFRNRLDAIVNFRPLDEEIILRVVDKFLLQLEGQLAE
                     KKVEVTFSDALRKYLGKKGFDPLMGARPMQRLIQDTIRRALADELLFGRLVDGGRLSV
                     DMDDKDEVVLDIQPSGKSGDRPKAEAAAL"
     gene            complement(2345..2698)
                     /gene="clpS"
                     /locus_tag="VF453_16495"
     CDS             complement(2345..2698)
                     /gene="clpS"
                     /locus_tag="VF453_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX7689314.1"
                     /translation="MPEENPPTPPLPPQPVTPDSDQAVVAERKAARTKPPRMYQVVLL
                     NDDFTPMEFVVMVLQEYFKLDLETATQIMLKIHHEGRGVCGLFTKDIAATKVELVLAA
                     ARRYGHPLQCIMEAA"
BASE COUNT          412 a          976 c         1010 g          555 t
ORIGIN      
        1 tcgcgtccgc cgggctcgtc gtctcgggga cgggccggtc agagggccgc ggcctccgcc
       61 ttcggacggt cgccgctctt gcccgagggc tggatgtcga gcaccacctc gtccttgtcg
      121 tccatgtcga cgctcaggcg gccgccgtcc acgaggcggc cgaacagcag ttcgtcggcc
      181 agtgcgcggc ggatcgtgtc ctggatgagg cgctgcatcg gccgcgcacc catgagcggg
      241 tcgaagccct tcttgccgag gtacttgcgc agggcgtcgg agaaggtgac ctcgaccttc
      301 ttctcggcca gctggccctc gagctgcagc aggaacttgt cgacgacgcg caggatgatc
      361 tcctcgtcca gcggccggaa gttcacgatc gcgtccaggc ggttgcggaa ctcgggcgtg
      421 aacaggcgct tgatgtcggc catctcgtcg ccctgctcgc gcttggtcgt gaagccgatc
      481 gtcgacttgt tcatcgtctc ggcgcccgcg ttcgtcgtca tgatgatgat gacgttgcgg
      541 aagtcggcct tgcgcccgtt gttgtccgtc agcgtgccgt ggtccatgac ctgcagcagc
      601 acgttgaaga cgtccgggtg agccttctcg atctcgtcga gcagcagcac gcagtgcggc
      661 ttcttggtga cctgctcggt cagcaggccg ccctggtcga agccgacgta gcccgggggc
      721 gcgccgatca ggcggctgac ggcgtggcgc tccatgtact ccgacatgtc gaagcggatc
      781 aggtcgatgc cgaggatgaa cgcgagctgc ttggcgacct ccgtcttgcc gacgcccgtc
      841 gggccggaga acaggaacgc gccgatcggc ttgtccggct tgcccaggcc cgagcgcgcc
      901 atcttgatcg cggccgacag ggcgtcgatg gccggatcct ggccgaagac gacgctcttc
      961 aggtcgcggt cgatggtgcg caggcggtcg cggtcgtccg tcgataccga ggccggcggg
     1021 atgcgcgcga tcttggcgac gatctcctcg acctcgttgc gcgtgatggt cttcttctgc
     1081 ttggacttcg gcaggatgcg ctgcgcggcg ccggcctcgt cgatcacgtc gatggccttg
     1141 tccggaaggt ggcggtcgtt gatgtacttg gccgacagct ccgccgcggc ctgcagcgcc
     1201 ccgagggcga acttgacgct gtggtgctcc tcgaagcgcg acttcaggcc cttcaggatc
     1261 tccaccgtct gctcgatcgt cggctcgttg acgtcgacct tctggaagcg ccgcgacagc
     1321 gccgcatcct tctcgaagat gccgcggtac tcggtgaagg tcgtcgcgcc gatgcacttc
     1381 atcgtgccgt tggacagcgc cggcttgagc aggttgctcg cgtccagcgt gccgcccgac
     1441 gcggcgcccg cgccgatcag cgtgtggatc tcgtcgatga acagcaccgc gcggggctgg
     1501 tccttgagct gcttgaggac gcccttcagg cgctgctcga agtcgccgcg gtatttggtg
     1561 ccggccagca acgcgcccat gtcgagcgag tagacgaccg cgtccgcgag cacctcgggc
     1621 acgtcgttct gcgtgatgcg ccacgcgagg ccctcggcga tcgccgtctt gcccacgccg
     1681 gcctcgccaa ccagcagcgg gttgttcttg cgccggcggc acaggatctg gatcacgcgc
     1741 tcgacctcgt gctcgcggcc gatcagcgga tcgatcttgc cgtcgcgcgc catctggttg
     1801 aggttctgcg tgaactgctc gagcggggag cccttgccct cgccgccctc gtccttctcg
     1861 gcggcctcgc cgccgccctc gccggccgac ttcggcggct ccggcggatc ggacttcttg
     1921 atgccgtggg cgatgtagtt gacgacgtcc aggcgcgtca cgccctgctg gtgcaggtag
     1981 tagacggcgt gcgagtcctt ctcgccgaag atggccacca gcacgttggc gccggtgact
     2041 tccttcttgc cgccgccggt ggactgcacg tgcatgatcg cgcgctggat cacgcgctgg
     2101 aacccgagcg tcggctgggt gtcgacctcc tcggagccgc cgaccgtggg cgtgttctcg
     2161 cggatgaagg tggacaggct ctttcgcagg tcctcgatgt tggccgcgca ggcgcgcagc
     2221 acctcggcgg ccgacgggtt gtcgagcagc gcgagcagca ggtgctccac ggtgatgaat
     2281 tcgtgccgct gctgacgtgc ctcgacaaac gccatgtgca ggctgacttc cagttcttgc
     2341 gcaatcatgc ggcctccata atgcattgaa gcgggtgtcc gtagcgacgt gcagcggcca
     2401 gcaccaactc gaccttcgtg gcggcgatgt ccttggtgaa cagcccgcag acgccgcgac
     2461 cctcgtggtg gatcttgagc atgatctgcg tggcggtctc gaggtcgagc ttgaagtact
     2521 cctgcagcac catcacgacg aactccatcg gcgtgaagtc gtcgttcagc agcaccacct
     2581 ggtacatgcg cggcggcttg gtgcgggcag ccttgcgctc tgccacgacg gcctgatcgg
     2641 agtcaggcgt gacgggctgc ggcggcagcg gcggcgttgg agggttttcc tcaggcatga
     2701 aatcgattct atagactcgc acgcgtccgg acacgatcgg catatcgtcg ccttgacgag
     2761 gtgtcgatca ttgccggata aagtcgactc aggggcagca tttcgggacg agccccctca
     2821 attcaagaac ggcgtggcag gccgcgcgcc cggcggtcga gcggccggcc cgcagcctgc
     2881 cggccagtcc gcttgcaggt gccgtgccaa ccgccgcgcc gccggcgcga ggctcgcgaa
     2941 cgagggcgcg acc
//
LOCUS       DASVLN010000699         6733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_89693, whole genome shotgun sequence.
ACCESSION   DASVLN010000699 DASVLN010000000
VERSION     DASVLN010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89693"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VF453_16500"
     CDS             complement(<1..1131)
                     /locus_tag="VF453_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689315.1"
                     /translation="MLGSSGRARRPGSALACAAIAFGLAACGGGGGGSSDPPGGGGTT
                     PVGPESALLLADFVAQDVNHQYVRVWDPARPGTAVETVQISSSNGTVWTSSHLVFSDA
                     TQVDPATRTTTTTGHVRAFFDNDGKLYTIDLRGGQPHAPVQLSSATDVTSPVSVTTMS
                     ADGTDAWIDARGGAHDWAIRSTMGAADAPISILGIVAPLRDASTGLPQYLFTSYSTRS
                     GTAMTPTTWAIVDASFHAVAEPTVATMGTYDGWVGADPSIAGLGYLRVSNALYAIRWS
                     AGGVAVDAAHDLHDFGAALLPPPATADAQSLYVADLRTLLAIGNGVVRTVGTFSGTPA
                     ALTDAGSYVVATLAPSATQASAETLRKSDGAYALLEPATAGLQ"
     gene            1506..3428
                     /locus_tag="VF453_16505"
     CDS             1506..3428
                     /locus_tag="VF453_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX7689316.1"
                     /translation="MDVISQFAARYERTREEELSLEEYLAECKRNPLAYATAAERMLK
                     AIGDPQTIDTRNDPRLSRLFANKVIKIYPAFAEFYGMEDAIEQVVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSIAERLKALMEQVPFYAIKGSPVNESPLGLFNATEDGPILESE
                     YGIPRRYLNRIMSPWAVKRLEEYGGDIRKFRVVKRRPSILKQVGISKTEPGDENNQDI
                     SSLVGKVDIRKLESYAQDDPDAYSYSGGLCLSNQGLLEFVEMFKAPIKVLHPLLTATQ
                     EGNFKGTEGFGAIPFDGIVLAHSNESEWKTFRNNKNNEAFLDRIYIVKVPYCLRVSEE
                     VKIYEKLIRGSSLAEATCAPGTLKMMAQFAMLTRLKEPENSSLYSKMQIYDGENLKDT
                     DPRAKSYQEYRDYAGVDEGMSGISTRFAYKILSKVFNFDSAEVAANPVHLMYVLEQQI
                     EREQFPQEVEQKYISFIKENLAPRYAEFIGKEIQTAYLESYSEYGQNIFDRYVTYADY
                     WIQDQEYRDTDTGEVFDRAALNAELEKIEKPAGISNPKDFRNEIVNFVLRARAQNAGK
                     NPAWTSYEKLRVVIEKKMFSNTEELLPVISFNAKASADEARKHEDFVNRMVQKGYTPK
                     QVRLLCEWYLRVRKSS"
     gene            3612..4895
                     /locus_tag="VF453_16510"
     CDS             3612..4895
                     /locus_tag="VF453_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEX7689317.1"
                     /translation="MLQQIIDRRLSGKNKSVGNRERFLRRHKEQIREAVRRAVDGRGI
                     RDLEKGEDVHIPKRDLSEPVFGHGQGGRREAVHPGNQEYVTGDRIKRPDGGGGGGGGG
                     SQASDSGEGEDDFVFHLSKEEFMQAFFDDLALPNLTRTQLAEIPEYKSHRAGFVNDGT
                     PSNLSIVRSMRGALGRRIALSADTRQELHDAERQLDELERQGADDAVTRKHKAELVEK
                     IQALRARVARVPFLDPLDLKFRNRVRVPVPTTRAVMFCLMDVSGSMDESRKDLAKRFF
                     ILLYLFLTRHYEKIEVVFIRHHTQASEVDEQNFFHATETGGTVVSSALVLMDEIIRQR
                     YPSSEWNIYGAQASDGDNWHHDSGRCRELLVDKILPMCRYFAYVQVAEPEQNLWEEYS
                     RIQGEVPHFAMRKAIEASQIYPVFRELFRKEGAQA"
     gene            5045..6505
                     /locus_tag="VF453_16515"
     CDS             5045..6505
                     /locus_tag="VF453_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX7689318.1"
                     /translation="MIERYHEVIRDTAGRFGLDTYPNQLEIITSEQMMDAYASVGMPV
                     NYRHWSYGKEFIATEKNYKRGQMGLAYEIVINSDPCISYLMEENTMAMQALVIAHAAY
                     GHNSFFKGNYLFRMWTDASSIIDYLVYSKDYVARCEERHGIEAVEELLDSCHALMNHG
                     VDRYRRPSKLSLAQEQARREDREAYAQLQVNDLWRTLPRRADKDDAAAPERRFPSEPQ
                     ENLLYFIEKNAPLLEPWQREIVRIVRKIAQYFYPQRQTQVMNEGWATFWHHRLLNQMY
                     DDGYLADGMMIEWLKSHTNVIAQPPVTSKYYSGINPYALGFAMYTDIRRVCENPTDED
                     RHWFPDFAGTPWLPTLDHAMRNFKDESFIGQYLSPKLMRDFRLFAIVDDEKDSELEIS
                     AIHDESGYRRLREALSRQYDLGSREPNIQVWNVNLRGDRSLTLRHTQHNDRPLHETSQ
                     EVLKHVARLWGFDVHLESVNGKGDTTRRWTVTPPKS"
BASE COUNT         1234 a         2333 c         2219 g          947 t
ORIGIN      
        1 ctgcagcccg gcggtggccg gctccagcag cgcgtaggcc ccgtcggact tgcgcagcgt
       61 ctccgcggac gcctgcgtcg ccgacggcgc cagcgtcgcg accacgtagg agccggcgtc
      121 cgtgagcgcg gccggcgtgc cggagaaggt ccccaccgtg cgcacgacgc cgttgccgat
      181 cgccagcagc gtgcgcaggt cggccacgta cagcgactgc gcgtcggccg tcgccggcgg
      241 cggcagcagc gcagcgccga agtcgtgcag gtcgtgcgcg gcgtcgaccg cgacgccgcc
      301 ggcgctccag cggatcgcgt acagcgcgtt ggagacccgc aggtagccca ggcccgcgat
      361 ggacgggtcg gccccgaccc agccgtcgta ggtgcccatc gtcgcgaccg tgggctcggc
      421 cacggcgtgg aacgaggcgt cgacgatcgc ccaggtggtg ggggtcatcg ccgtgcccga
      481 gcgcgtgctg tacgaggtga acaggtactg cggcaggccg gtggacgcgt cgcgcagcgg
      541 cgccacgatg cccaggatcg agatcggcgc gtcggcggcg cccatggtcg accggatcgc
      601 ccagtcgtgc gcgccgcccc gggcgtcgat ccaggcgtcc gtgccgtcgg ccgacatcgt
      661 cgtcaccgag acgggcgagg tcacgtcggt ggccgacgac agctgcaccg gcgcatgcgg
      721 ctggccgccg cgcaggtcga tggtatagag cttgccgtcg ttgtcgaaga acgcgcgcac
      781 gtggccggtg gtcgtcgtcg tccgggtcgc cggatcgacc tgcgtcgcgt cggagaagac
      841 gagatgggag gacgtccaca ccgtgccgtt gctcgacgag atctgcaccg tctccaccgc
      901 ggtgcccggg cgcgccggat cccagacgcg cacgtactgg tggttgacgt cctgggccac
      961 gaagtcggcg agcagcaggg ccgattccgg cccgaccggc gtcgtgccgc cgcctccggg
     1021 aggatccgag ctgccaccgc caccaccgcc gcaagccgcc agcccgaagg cgatcgccgc
     1081 gcacgcgagc gcgcttcccg gccggcgcgc gcgccccgag cttccaagca tcgtttcgat
     1141 tcccccgtgc cgtttcgtga actgcggccg gcgggcgggc cgcggacgcg tcgacttaaa
     1201 cctacagtgg ttgaaatgta ggcagggaaa ggacaagatg gaaccggaac aacaccctat
     1261 tcgacccttt gggggtcacg aactcgtcgc acgctgtaac ctcgggaatc gacgttcaga
     1321 cgctcgccgc tgaccggatt gctctgatgg cgccttgacc ttcgctcagt acttgcccta
     1381 gagaacgaag cgctcggtcc ggactccatc aaaacattcc agacgcctct gccgcacggg
     1441 ttgcaagccc gcaaccgcat tcgcacgcag aagcgccgtg tttccattca gccaggaggt
     1501 cagacatgga cgtgatcagc cagttcgcag cccgctacga acgcacccgc gaagaggaac
     1561 tctccctcga ggagtacctc gccgagtgca agcgcaaccc cctggcttac gcgacggccg
     1621 ccgagcgcat gctcaaggcc atcggcgacc cgcagaccat cgacacgcgc aacgacccgc
     1681 gcctgtcgcg cctgttcgcc aacaaggtca tcaagatcta tcccgccttc gccgagttct
     1741 acggcatgga ggacgcgatc gagcaggtcg tctcctactt ccgccacgcc gcgcagggcc
     1801 tggaggagaa gaagcagatc ctctacctgc tcggcccggt gggcggcggc aagagctcca
     1861 tcgccgagcg gctgaaggcg ctgatggagc aggtgccgtt ctacgcgatc aagggctcgc
     1921 cggtgaacga gtcgccgctc ggcctgttca acgccacgga ggacggcccg atcctcgaga
     1981 gcgagtacgg catcccgcgc cgctacctca atcgcatcat gtcgccgtgg gcggtcaagc
     2041 ggctcgagga gtacggcggc gacatccgca agttccgcgt cgtcaagcgc cgcccgtcga
     2101 tcctcaagca ggtcggcatc agcaagaccg agccgggcga cgagaacaac caggacatct
     2161 cgtcgctggt cggcaaggtc gacatccgca agctcgagag ctacgcgcag gacgacccgg
     2221 acgcgtacag ctactccggc ggcctgtgcc tgtccaacca gggcctgctc gagttcgtcg
     2281 agatgttcaa ggcgccgatc aaggtgctgc acccgttgct gacggccacg caggaaggca
     2341 acttcaaggg caccgagggc ttcggcgcga tcccgttcga cggcatcgtg ctcgcgcact
     2401 ccaacgagag cgagtggaag accttccgca acaacaagaa caacgaggcc ttcctcgacc
     2461 gcatctacat cgtcaaggtg ccgtactgcc tgcgcgtgag cgaggaagtc aagatctacg
     2521 agaagctgat ccgcggctcg tcgctcgccg aggccacctg cgcgccgggc acgctgaaga
     2581 tgatggcgca gttcgcgatg ctcacgcgtc tgaaggagcc cgagaactcg tcgctgtact
     2641 cgaagatgca gatctacgac ggcgagaacc tcaaggacac cgacccgcgc gccaagagct
     2701 accaggagta ccgcgactac gccggcgtcg acgagggcat gagcgggatc tcgacgcgct
     2761 tcgcgtacaa gatcctgtcc aaggtcttca acttcgattc cgccgaggtc gccgccaacc
     2821 cggtgcacct gatgtacgtg ctcgagcagc agatcgagcg cgagcagttc ccgcaggagg
     2881 tcgagcagaa gtacatctcc ttcatcaagg agaacctggc cccgcgctac gcggagttca
     2941 tcggcaagga gatccagacg gcgtacctcg agagctactc ggagtacggc cagaacatct
     3001 tcgaccgcta cgtgacctac gccgactact ggatccagga ccaggagtac cgcgacaccg
     3061 acacgggcga ggtcttcgac cgcgcggcgc tgaacgccga gctcgagaag atcgagaagc
     3121 ccgcgggcat ctccaacccg aaggacttcc gcaacgagat cgtcaacttc gtgctgcgcg
     3181 cccgcgccca gaacgccggc aagaacccgg catggacgag ctacgagaag ctgcgcgtcg
     3241 tgatcgagaa gaagatgttc tccaacacgg aggagctgct gcccgtcatc agcttcaacg
     3301 cgaaggcgag tgccgacgag gcgcgcaagc acgaggactt cgtcaaccgc atggtgcaga
     3361 agggctacac gcccaagcag gtgcgcttgc tttgcgagtg gtacctgcgg gtgcgcaaga
     3421 gctcctgagg tttccccgtt tgacggcatt tcaacgacag ccgttccgtc gcctcgtgat
     3481 gctcccggcg ctgccgctcg cgcgccctgc ggggagttca agggagcgcc ggatccggct
     3541 ccgccgggcc ggtcgcggag ccccccctcg gggggcagcg agcgccagcg agcgtggggg
     3601 ctaacatctt tatgcttcag cagatcatcg atcgacgcct gtccggcaag aacaagtcgg
     3661 tcggcaaccg cgagcgcttc ctgcgccgcc acaaggagca gatccgcgag gcggtgcggc
     3721 gcgcggtgga cggccgcggc atccgcgacc tggagaaggg cgaggacgtc cacatcccca
     3781 agcgcgacct cagcgaaccc gtcttcggcc acggccaggg cggccggcgg gaggcggtgc
     3841 atccgggcaa ccaggaatac gtcaccggcg accgcatcaa gcgccccgac ggtggcggag
     3901 gcgggggcgg gggcggcagc caggcgagcg actccggcga gggcgaggac gacttcgtct
     3961 tccacctgtc gaaggaggag ttcatgcagg ccttcttcga cgacctcgcg ctgcccaacc
     4021 tgactcgcac ccaactcgcc gagatccctg aatacaagtc gcaccgcgcc ggcttcgtca
     4081 acgacggcac gcccagcaac ctgagcatcg tgcgctcgat gcgcggcgcg ctcggccggc
     4141 gcatcgcgtt gtcggccgac acccgccagg agctgcacga cgccgagcgc cagctcgacg
     4201 agctggaacg ccagggcgcc gacgacgccg tcacgcgcaa gcacaaggcc gagctggtcg
     4261 agaagatcca ggcgctgcgg gcacgggtcg cgcgcgtgcc cttcctcgat ccgctggacc
     4321 tgaagttccg caaccgcgtg cgcgtgccgg tgcccacgac gcgggccgtg atgttctgcc
     4381 tgatggacgt ctcgggctcc atggacgagt cgcgcaagga tctcgccaag cgcttcttca
     4441 tcctgctgta cctgttcctc acgcggcact acgagaagat cgaggtggtc ttcatccgcc
     4501 accacacgca ggcctcggag gtcgacgagc agaacttctt ccacgcgacc gagaccggcg
     4561 gcacggtggt ctccagcgcg ctggtgctga tggacgagat catccgccag cgctatccgt
     4621 ccagcgaatg gaacatctac ggcgcgcagg cgagcgacgg cgacaactgg caccacgaca
     4681 gcggccgctg ccgcgagctg ctggtcgaca agatcctgcc catgtgccgc tacttcgcct
     4741 acgtgcaggt cgccgagccc gagcagaacc tgtgggagga atacagccgc atccagggcg
     4801 aggtgccgca cttcgcgatg cgcaaggcga tcgaggcatc gcagatctac cccgtgttcc
     4861 gcgagctgtt ccgcaaggaa ggagcgcagg catgagcttc cccgagaacc ggcgcgcggt
     4921 cggtcgcaac gcgagcgccg acgagtacgg cggccgccgg cgccaggggc cgccgcagga
     4981 gatcgcgcct ctgaacgagc gccccgcgca gccgctgccc tcgccgagcg actggacctt
     5041 cgacatgatc gagcggtacc acgaggtgat ccgcgacacg gcgggccgct tcgggctcga
     5101 cacgtatccg aaccagctcg agatcatcac ctccgagcag atgatggacg cctacgccag
     5161 cgtgggcatg ccggtgaact accgccactg gagctacggc aaggagttca tcgccaccga
     5221 gaagaactac aagcgcggcc agatgggcct cgcctacgag atcgtcatca actcggaccc
     5281 gtgcatctcc tacctgatgg aggagaacac gatggcgatg caggcgctcg tcatcgcgca
     5341 cgcggcctac ggccacaaca gcttcttcaa gggcaactac ctgttccgga tgtggacgga
     5401 cgcgtcgtcg atcatcgact acctcgtcta ctcgaaggac tacgtcgccc gctgcgagga
     5461 gcgccacggc atcgaggcgg tcgaggagct gctcgactcc tgccacgcgc tgatgaacca
     5521 cggcgtcgac cgctaccggc ggccgagcaa gctctcgctc gcgcaggagc aggcgcggcg
     5581 cgaggatcgc gaagcctacg cccagctgca ggtcaacgac ctctggcgca cgctgccgcg
     5641 ccgcgccgac aaggacgacg ccgccgcgcc cgagcgccgc ttcccgagcg agccgcagga
     5701 gaacctgctg tacttcatcg agaagaacgc gccgctgctg gagccgtggc agcgcgagat
     5761 cgtgcgcatc gtgcggaaga tcgcgcagta cttctacccg cagcgccaga cgcaggtgat
     5821 gaacgagggc tgggcgacgt tctggcacca ccggctgctc aaccagatgt acgacgacgg
     5881 ctacctcgcc gacggcatga tgatcgagtg gctgaagtcg cacaccaacg tgatcgcgca
     5941 gccgcccgtg acctcgaagt actactcggg catcaacccg tacgcgctgg gcttcgcgat
     6001 gtacaccgac atccgccgcg tctgcgagaa cccgaccgac gaggatcgcc actggttccc
     6061 cgacttcgcg ggcaccccct ggctgccgac gctggatcac gcgatgcgca acttcaagga
     6121 cgagagcttc atcggccagt acctctcgcc caagctgatg cgcgatttcc ggctgttcgc
     6181 catcgtcgac gacgagaagg attccgagct cgagatctcc gcgatccacg acgagagcgg
     6241 ctaccgccgg ctgcgcgagg cgctgtcgcg gcagtacgac ctcggctcgc gcgagcccaa
     6301 catccaggtc tggaacgtga acctgcgcgg cgaccgctcg ctcaccctgc gccacacgca
     6361 gcacaacgac cggccgctgc acgagacctc gcaggaggtg ctcaagcacg tggcgcggct
     6421 gtggggcttc gacgtgcatc tcgagagcgt caacggcaag ggcgacacga cccgccggtg
     6481 gacggtcacg ccgcccaaga gctgagccgc gccggaaggc acgggcggcc gccgcgcgcg
     6541 gccgtcgccg tttgcgcggt ggcacgccgg ggcgtcgcgg cgcgcggcca caatgcggcc
     6601 attgcagcgc ctgccatcga catgcacgtc cgcgacttcg accccgtgac cgacatcccc
     6661 gccgtcgccg cgctgttcgg ccagctcggc tacccgaccg agcccgaggc gttgcgcgcg
     6721 cgggcggacg cgg
//
LOCUS       DASVLN010000700         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_250777, whole genome shotgun sequence.
ACCESSION   DASVLN010000700 DASVLN010000000
VERSION     DASVLN010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250777"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF453_16520"
     CDS             complement(<1..327)
                     /locus_tag="VF453_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689319.1"
                     /translation="MAADRDPEPLVAVCATFEDARQLAETLAAGGLDPAQLSVVGSRE
                     GLPDAPTMEARLRKWSRLGSLWGALGGLAAGVVLAVPAVGAVVASGPIAAMLLVAAEG
                     AVLGSGL"
     gene            complement(381..758)
                     /locus_tag="VF453_16525"
     CDS             complement(381..758)
                     /locus_tag="VF453_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7689320.1"
                     /translation="MSTGHVSYPRLTREISARIAGLRESQPAAMHGFRRLSAGAMDAG
                     SLTAKHKELMALALGIAARCEGCIGFHAKALVAMGATRGEIEETVAVAVYMGGGPALM
                     YGASAIDAYEQFLAEASTHAHPA"
     gene            complement(871..1794)
                     /locus_tag="VF453_16530"
     CDS             complement(871..1794)
                     /locus_tag="VF453_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX7689321.1"
                     /translation="MRCAYVTHADCMRHEMGAGHPESPARLAAIHDRLLFDGLLDFLS
                     VHDAPLATQEQLCRAHAPEHVAHILASTPIEGYVAIDPDTCMNPHSAIAALRAAGAVV
                     LATDLVLAGQADTAFCCVRPPGHHAERAAAMGFCFFDNVAVGIRHALEVHGLERVALV
                     DFDVHHGNGSQDIFRDDPRVLMCSTYEIDLYPFPHDTSGANMINVGLPPRSGGPAFRD
                     AVASRWLPAMEDFAPQLVVVSAGFDGHRADDMGNLGLVEADFQWVTRELLALARRHCG
                     GRIVSSLEGGYHLPSLARSVAAHVAVLAGAD"
     gene            complement(1803..2258)
                     /locus_tag="VF453_16535"
     CDS             complement(1803..2258)
                     /locus_tag="VF453_16535"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7689322.1"
                     /translation="MYSRILVPVDGSVPSRCGLREALGLAADGRATVVLLHVAAEPAL
                     LMGAAGYVNYGELSDMLRRAGQEIVDDAAREAAKAGVPCETQVVDAGAGAVSDAIVDQ
                     ASRQRCQLIVMGTHGRRGVRRLTLGSDAELVVRHAPVPVLLVKAPEGTA"
     gene            complement(2365..3885)
                     /locus_tag="VF453_16540"
     CDS             complement(2365..3885)
                     /locus_tag="VF453_16540"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7689323.1"
                     /translation="MLYAQPNTPGAPIEFKPRYDNFIGGRFVPAADGRAFDVITPITG
                     RVYTQVARSGAEDIERALDAAHAAAAAWGATSPAERANVLLKIADRIEQNTERLAWAE
                     TVDNGKPMRETLNADIPLAVDHFRYFAGCLRAQEGAISEIDRNTVAYHFHEPLGVVGQ
                     IIPWNFPILMAAWKLAPALGAGNCVVLKPAESTPVSILVLAELVADLLPPGVLNIVNG
                     YGAEAGLPLAQSKRIAKIAFTGSTATGRTIAQAAAGNLIPATLELGGKSPNIFFDDVM
                     AADDDFLDKAIEGLVLFAFNQGEVCTCPSRALVQASIFDRFMARALERVNAIRQGNPL
                     DTATMIGAQASAAQQDKILSYLDVGRREGAKVLAGGAAAKMPGDLAGGFYIQPTVLQG
                     HNRMRVFREEIFGPVLAVTTFTDEADALAIANDTPYGLGAGVWTRDGSRAYRMGRGIQ
                     AGRVWTNCYHAYPAHAAFGGYKESGIGRETHKQMLDHYQQTKNLLVSYSPGALGFF"
BASE COUNT          547 a         1485 c         1556 g          561 t
ORIGIN      
        1 caggccgctg cccagcaccg cgccctcggc cgcgaccagc agcatcgccg cgatcggtcc
       61 gctcgcgacg accgcgccga ccgccggcac cgcgaggacg accccggcgg cgaggccgcc
      121 cagggcgccc cacaggctgc cgaggcggct ccacttgcgc agccgggcct ccatcgtggg
      181 ggcatcgggc aggccctcgc ggctgccgac caccgacagc tgcgcgggat cgaggccgcc
      241 ggcggcgagc gtctcggcca gctgccgcgc gtcctcgaac gtggcgcaga cggccacgag
      301 cggctcggga tcgcggtcgg cggccatcgg ctccaggtgg atcgaggaag ggcgaggccg
      361 cgcggcggtc ggaccgcggc tcacgccggg tgcgcgtgcg tcgacgcctc ggcgaggaac
      421 tgctcgtagg cgtcgatcgc gctcgcgccg tacatcagcg ccggtccgcc gcccatgtag
      481 acggccacgg cgacggtctc ctcgatctcg ccgcgcgtcg cgcccatcgc caccagcgcc
      541 ttggcatgga agccgatgca gccctcgcag cgggccgcga tgccgagtgc gagcgccatc
      601 agctccttgt gcttcgccgt caggctgccc gcgtccatcg cgcccgcgga gaggcggcga
      661 aagccgtgca tcgccgcggg ctggctctcg cgcaggccgg cgatgcgcgc ggagatctcg
      721 cgcgtcagcc ggggatagga cacgtggccg gtgctcatgg gatcctctcg gaaggcggcg
      781 cgacggaccg ggtgccgcgc agccgcctcg cgcggacgaa gccatcatgc gcccccgcgc
      841 gccgcgtgcc ttgcgttgcc gcaagcgccg tcaatcggcc cccgccagca ccgcgacgtg
      901 cgcggccacg ctgcgcgcga ggctcggcag gtggtagccg ccttccagcg acgacacgat
      961 gcggcccccg cagtgccgcc gcgcgagcgc gagcagctcg cgcgtcaccc actggaagtc
     1021 ggcctcgacg aggccgagat tgcccatgtc gtccgcgcgg tggccgtcga agccggccga
     1081 gacgacgacc agctgcggcg cgaagtcctc catcgcgggc agccagcggc tcgcgaccgc
     1141 gtcgcggaac gccggcccgc cggagcgcgg cggcagcccg acgttgatca tgttcgcgcc
     1201 ggaggtgtcg tgcgggaacg ggtagaggtc gatctcgtag gtcgagcaca tcagcacgcg
     1261 cggatcgtcg cggaagatgt cctggctgcc gttgccgtgg tggacgtcga agtcgaccag
     1321 cgcgacgcgc tccagcccgt gcacctccag cgcatggcgg atgcccaccg ccacgttgtc
     1381 gaagaagcag aagcccatcg ccgcggcgcg ctcggcgtgg tggcccggcg gccggacgca
     1441 gcagaacgcg gtgtcggcct ggcccgcgag cacgaggtcg gtggcgagga cgacggcgcc
     1501 ggccgcccgc agcgcggcga tcgccgagtg cgggttcatg caggtgtcgg ggtcgatcgc
     1561 gacgtagccc tcgatcggcg tcgacgcgag gatgtgcgcg acatgctcgg gcgcatgcgc
     1621 gcggcacagc tgctcctgcg tcgcgagcgg cgcgtcatgg acgctcagga agtccagcag
     1681 cccgtcgaac agcaggcggt cgtggatggc ggccagccgg gccgggcttt cggggtggcc
     1741 ggcgcccatc tcgtggcgca tgcagtcggc gtgcgtgacg taggcgcagc gcatcgccgg
     1801 tctcaggcgg tcccctcggg cgccttcacg agcagcacgg gcaccggggc gtggcgcacg
     1861 accagctcgg catcgctgcc cagcgtcagg cgccggacgc cgcgccgccc gtgcgtgccc
     1921 atgacgatca gctggcagcg ctggcgcgag gcctggtcca cgatcgcgtc ggagaccgcg
     1981 ccggcgccgg cgtccaccac ctgcgtctcg cagggcacgc cggccttcgc ggcctcgcgc
     2041 gccgcgtcgt cgacgatctc ctgcccggcg cgccgcagca tgtcggacag ctcgccgtag
     2101 ttgacgtagc cggccgcgcc catcagcaac gcaggctcgg cggccacgtg cagcaggacc
     2161 accgtggcac ggccgtccgc ggcaaggccg agggcctcgc gcaggccgca gcgcgacggc
     2221 acgctgccgt cgacgggaac gaggatgcgt gaatacatgg aaggctccgg gcgccggccg
     2281 atccggcgtg aagccattgt tcgcatccgg cgcgtgaacg gccttgcgac agctcaatcg
     2341 gcccgcctcc cgccgccggg cgcgtcagaa gaagccgagc gcgccgggcg agtagctcac
     2401 caggaggttc ttggtctgct ggtagtggtc gagcatctgc ttgtgcgtct cgcggccgat
     2461 gcccgattcc ttgtagccgc cgaaggccgc gtgcgccggg taggcgtggt agcagttcgt
     2521 ccagacgcgc ccggcctgga tgccccgtcc catgcggtac gcgcggctgc cgtcgcgcgt
     2581 ccacacgccg gcgccgaggc cgtacggcgt gtcgttggcg atcgcgaggg cgtcggcctc
     2641 gtcggtgaac gtcgtcacgg cgaggaccgg gccgaagatc tcctcgcgga acacccgcat
     2701 ccggttgtgg ccctgcagca ccgtcggctg gatgtagaag ccgcccgcga gatcgcccgg
     2761 catcttcgcc gcggcgccgc cggcgagcac cttcgcgccc tcccggcggc cgacgtccag
     2821 gtacgacagg atcttgtcct gctgcgccgc gctggcctgc gcgccgatca tcgtcgcggt
     2881 atcgagcggg ttgccctggc ggatcgcgtt gacgcgctcg agggcgcgcg ccatgaagcg
     2941 gtcgaagatc gacgcctgca ccagcgcccg gctcgggcag gtgcacacct cgccctggtt
     3001 gaacgcgaac agcacgaggc cctcgatcgc cttgtccagg aagtcgtcgt cggcggccat
     3061 cacgtcgtcg aagaagatgt tgggactctt gccgcccagc tccagcgtcg ccgggatgag
     3121 attgccggcc gccgcctgcg cgatcgtgcg gccggtggcc gtgctgccgg tgaacgcgat
     3181 cttggcgatg cgcttgctct gcgcgagcgg caggccggcc tcggcgccgt agccattgac
     3241 gatgttgagc acgcccggcg gcagcaggtc ggcgacgagc tcggccagga cgaggatgct
     3301 gaccggcgtc gattcggccg gcttgagcac gacgcagttg cccgcgccca gcgccggcgc
     3361 gagcttccac gccgccatca ggatcgggaa gttccagggg atgatctgcc cgacgacgcc
     3421 caggggctcg tggaagtggt aggcgacggt gttgcggtcg atctcgctga tcgccccctc
     3481 ctgcgcccgc aggcagccgg cgaagtagcg gaagtgatcg accgccagcg ggatgtcggc
     3541 gttgagcgtc tcgcgcatgg gcttgccgtt gtccacggtc tccgcccatg cgaggcgctc
     3601 cgtgttctgc tcgatgcggt cggcgatctt cagcaggacg ttggcgcgtt cggccggcga
     3661 cgtggcgccc caggcggcgg ccgcggcgtg ggcggcgtcg agcgcgcgct cgatgtcctc
     3721 ggcgccggag cgcgcgacct gggtgtagac gcggccggtg atcggcgtga tcacgtcgaa
     3781 cgcgcggccg tcggcggccg gcacgaagcg gccgccgatg aagttgtcgt agcgcggctt
     3841 gaactcgatc ggggcgccgg gggtgttcgg ttgggcgtac agcatgcgtg tctccttgcg
     3901 gtcgtgggat gcggccctgg gacggcaggc atgccggccg gggccgcgcc gccttcgcat
     3961 ggcgcgtgcc agcgcgattc ggaccgcacc ggcgcgatcc cgcgcaagaa gtccgtcagg
     4021 cggccggcgc agactgtcgc gcgcgccgcg atgtcggcgc gacatgtcac gaaacgggac
     4081 aggcgccgcg ccgattcgcg gcggaccgct tgcggcgccg cgtgccggcg ctacgctggc
     4141 gcgcggacg
//
LOCUS       DASVLN010000701         5054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_467572, whole genome shotgun sequence.
ACCESSION   DASVLN010000701 DASVLN010000000
VERSION     DASVLN010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467572"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..836
                     /locus_tag="VF453_16545"
     CDS             147..836
                     /locus_tag="VF453_16545"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HEX7689324.1"
                     /translation="MTEPTRPRHRPELGYARGDQTRRRIVDAAIRLFGQRGFEGASTR
                     DIARLAGVNAPALQYYFDGKEGLYRACAEHIAETMAAHFEPALQAAEAVLARDAGREE
                     LIEAYLRLQDAMAEHLLTSPDAQDRRLFATHEQIGNGPGMLLEILDTMRPRLMRPSVA
                     IVARLTGRAEEDPLTLVRVMTLHGQMAVFHMGQRAKLDLIALPDDEQRFTLIREVVRA
                     QTRAVIGSWEG"
     gene            1389..1637
                     /locus_tag="VF453_16550"
     CDS             1389..1637
                     /locus_tag="VF453_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689325.1"
                     /translation="MFANTDALAGALIAVLSESRRLERLLASASLPDDEERRLMDRVE
                     QMHAAYGELAEEYERRLAHHPGLLSLDSLKARIASEFR"
     gene            complement(1742..1817)
                     /locus_tag="VF453_16555"
     tRNA            complement(1742..1817)
                     /locus_tag="VF453_16555"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1782..1784),aa:Phe,seq:gaa)
     gene            complement(1966..2667)
                     /locus_tag="VF453_16560"
     CDS             complement(1966..2667)
                     /locus_tag="VF453_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689326.1"
                     /translation="MATILVVDDEMGIRALLSEILTDEGHTVEVAENAASARLIRERL
                     RPDLVLLDIWMPDVDGITLLKEWGATAQLNMPVIMMSGHGTIDTAVEATKYGAIAFLE
                     KPITLQKLLRAVEQGLARPSQRGALPADNHALGGYRPEAMTVEAAMTGGLGLQPAAPL
                     NLGPQPEQSFDLDRPLREARDAFEKSYFEFHLARENGSMTRVAEKTGLERTHLYRKLK
                     QLGVDLSRGKRGIGA"
     gene            complement(2731..4995)
                     /locus_tag="VF453_16565"
     CDS             complement(2731..4995)
                     /locus_tag="VF453_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689327.1"
                     /translation="MSRASRRFTWAVSLIAFLGVALVLGFVVSLSGGEPSLYERNFVW
                     LFWLNFSVAMLLLLVLAAAAVRLAIRWHSGKFGSRLLLKLAAIFALVGVVPGALIYTV
                     SLQFVTRSIDAWFDTKVDNALVAGLNLGKGTLDTLSSDLVDKARYAAGRLGDPRSSGA
                     AITLERLRDQIGVQDLALVGGNGQILMSASSRPALLVDRPTAVLIRQARATGLSSQVE
                     GLEDEASPGTGQLARVRALVQVPSSGMSLDTTERFMLAVQPLPPVVASNALAVQAAYR
                     DYQQRALAREGLRRMYVGTLTLTVVLSVFGSVLIAVVFGNQIARPLLLLAEGMRQVAA
                     GDLGAKPVYESSDEIGGLTRSFAQMTEQLGQARADVDRSVMQLEGARTRLQTILDNLT
                     AGVILFDAQWRIDTVNPGATRILRAPLSAYQGRKLGELPGMADFAQAVEQRFELHLAA
                     PESGERLLWQEQFELKVGDKGQELALLVRGALLPGDARLMVFDDITEVVSAQRSAAWA
                     EVARRLAHEIKNPLTPIQLSAERLQFKLEPRLADASDQAMLQRSVSTIVAQVQAMKQL
                     VNEFRDYARLPSANLQAIDLNELVTEVLALYGDAQENGHLVARLEPGLPRIEGDATQL
                     RQVVHNLVQNALDAASIWPDALVQVRTESARKDDGSLRVVRLKVEDNGPGFPENVLQR
                     AFEPYVTTKSKGTGLGLAVVKKIVDEHRARVRITNLGRGEDGATGAAGAQVSISFSRF
                     APAATVEPPRETPV"
BASE COUNT          809 a         1711 c         1754 g          780 t
ORIGIN      
        1 cgggcagcga accggccgac ggcgaagtgg gagaggcagg cgaagtcatc gaggcaccaa
       61 aatattccag atggaaattt ttagcgttcg atagaaatct gtcaactggt atattttgcg
      121 gacggcgcaa tgccgccacc gaccccatga ccgaaccgac ccgcccccgc caccgccccg
      181 agctcggcta cgcgcgcgga gaccagacgc gccggcgcat cgtcgacgcc gcgatccgcc
      241 tgttcggcca gcgcggcttc gaaggcgcgt ccacgcgcga catcgcccgg ctggccggcg
      301 tcaacgcgcc cgcgctgcag tactacttcg acggcaagga aggcctgtac cgcgcctgcg
      361 ccgagcacat cgccgagacc atggccgcgc acttcgagcc ggcgctgcag gccgcggagg
      421 ccgtgctggc ccgcgacgcc ggccgcgagg agctcatcga ggcctacctg cgcctgcagg
      481 acgcgatggc cgaacacctg ctgacgtccc cggacgccca ggatcgacgc ctgttcgcca
      541 cccacgagca gatcggcaac ggccccggca tgctgctgga gatcctcgac acgatgcgcc
      601 cccgcctgat gcgccccagc gtggccatcg tcgcgcgcct gacgggccgg gccgaggaag
      661 acccgctgac cctcgtgcgc gtgatgacgc tgcacgggca gatggccgtg ttccacatgg
      721 gccagcgcgc gaagctcgac ctgatcgcgc tgcccgacga cgagcaacgg ttcacgctca
      781 tccgcgaggt ggtgcgggcg cagacgcggg cggtgatcgg gagttgggag ggctgaccgg
      841 ggcgatgaca ttggcctggt cgtgacgccg ctgcggccga aggcccggcc gtctgcgaca
      901 gccccgcctg ccatccggcc gaaccggcga tctgttctcg gcgttgaatt cattcccccc
      961 ggagtgcaac gaactcttct ccccgatgaa tgacgtcatc gccggacttc gatgactcaa
     1021 cgcgcggtgc aatgaattcg actccgcggc gagttcattg gatcgccggc tgaactcatt
     1081 catcgttgcg gtgaactcat tcgtttcggg attgaatcaa ttcattcgtc gacataattg
     1141 acgaaccaaa acggtgacat cgttatgtcg cgagtcgaat tcattccgtc ccacacggat
     1201 tgggcgaggc aggcgaagcc tcagagctct gaatgagcga cggctgccct gcgacccgca
     1261 tcatcagcaa cgcgcgccga ccttcgttca gaacgtcaca tggttcgaca gcaagacggc
     1321 caggcatttc atgcttcccg ccagcacccg gcattggctg ctcaaccaaa caactgaagg
     1381 gtagtctcat gttcgcaaac accgatgcac tcgcaggcgc tttgatcgcc gttctgagcg
     1441 aatccaggcg cctggaacgc ctgctcgcca gcgcatcact tcctgacgac gaggagcgac
     1501 ggctgatgga tcgcgtggag cagatgcacg cggcttacgg tgaactcgcc gaggagtacg
     1561 agcgccgcct ggcccatcat cccggactgt tgtcactgga ctcgctcaag gcgcgcattg
     1621 cgtcggagtt ccgatagctg gcgtacctga gcggtctgcc agtagaggct ctcagacctg
     1681 acgcggagcc aacaacagca aaaagcccct gattccgtga agaatcaagg gcttagctat
     1741 ttggtggcct ggggcggaat cgaaccaccg acacgcggat tttcaatccg ctgctctacc
     1801 aactgagcta ccaggccagt gtctttgcag acgtcttcgt cgatgcgttc caggcggagc
     1861 gaccagcgaa gcccactagt gtaccagctt tttcggtttc ggccgtgttg ccgaggaaaa
     1921 cgttgggtgg gcgggacgtg cgtgcgcctc gcgggcgcgc gtgggtcagg cgccgatgcc
     1981 gcgcttgccg cgcgagaggt cgacgccgag ttgcttgagc ttgcggtaca ggtgggtgcg
     2041 ctcgaggccg gtcttctcgg cgacgcgggt catcgagccg ttctcgcggg cgaggtggaa
     2101 ctcgaagtag ctcttctcga aggcgtcgcg ggcctcgcgc agcgggcggt cgaggtcgaa
     2161 gctctgttcg ggctgcgggc cgaggttcag cggggcggcc ggctgcaggc ccaggccgcc
     2221 ggtcatcgcg gcctcgaccg tcatcgcttc cgggcggtag ccgccgaggg cgtggttgtc
     2281 ggccggcagc gcgccgcgct gggacgggcg ggcaaggccc tgctcgaccg cgcgcagcag
     2341 cttctgcagc gtgatgggct tctcgaggaa tgcgatggcg ccgtacttgg tggcctcgac
     2401 ggcggtgtcg atggtgccgt ggccgctcat catgatgacg ggcatgttga gctgcgcggt
     2461 ggcgccccac tccttgagca gcgtgatgcc gtcgacgtcg ggcatccaga tgtcgaggag
     2521 gacgaggtcg ggccgcagcc gctcgcggat caggcgggcg ctggcggcgt tctcggccac
     2581 ctccaccgtg tggccttcgt cggtgaggat ctccgacagc agggcacgga tgcccatttc
     2641 gtcatcaaca acaagaatcg tggccatgaa tttggggacg gctgcgggtg tcggctccgc
     2701 gacgcgggcg catcggcagc gaagaacgct ttatacaggg gtttcgcggg gcggttcgac
     2761 ggtcgccgcc ggagcgaagc gcgaaaatga tatcgaaact tgggccccgg cggcgccggt
     2821 cgcgccgtct tcgccgcggc ccaggttcgt gatccggacg cgggcgcgat gttcgtcgac
     2881 gatcttcttg acgacggcca gcccgaggcc ggttcccttg gacttggtcg tgacgtaggg
     2941 ctcgaaggcg cgctggagca cattctcggg gaaaccgggc ccattgtcct ccaccttgag
     3001 ccgcacgacg cgcaggctgc cgtcgtcctt gcgggcggac tcggtgcgca cctgcaccag
     3061 cgcgtcgggc cagatcgagg cggcgtccag cgcgttctgc accaggttgt ggacgacctg
     3121 gcgcagctgg gtggcgtcgc cctcgatgcg cggcaggccc ggctcgaggc gggcgacgag
     3181 gtggccgttc tcctgcgcgt cgccgtacag ggcgagcacc tcggtcacca gctcgttgag
     3241 gtcgatcgcc tgcaggttgg cgctgggcag gcgcgcgtag tcgcggaact cgttgaccag
     3301 ctgcttcatc gcctgcacct gggcgacgat ggtcgagacc gagcgctgca gcatggcctg
     3361 gtcgctcgcg tcggccaggc gcggctcgag cttgaactgc agccgctccg ccgagagctg
     3421 gatcggcgtg agcggattct tgatctcgtg cgcgagccgg cgcgcgacct cggcccaggc
     3481 ggccgagcgc tgcgccgaga ccacctcggt gatgtcgtcg aacaccatca gccgcgcgtc
     3541 gccgggcagc agcgcgccgc gcaccagcag cgcgagctcc tggcccttgt cgccgacctt
     3601 gagctcgaac tgctcctgcc acagcagccg ctcgccgctc tccggcgccg ccaggtgcag
     3661 ctcgaagcgc tgctccacgg cctgcgcgaa atcggccatg cccggcagct cgcccagctt
     3721 gcggccctgg taggccgaca gcggcgcgcg caggatgcgc gtcgcgccgg ggttgacggt
     3781 gtcgatgcgc cactgcgcgt cgaacaggat cacgccggcg gtcaggttgt cgaggatggt
     3841 ctgcagccgc gtgcgcgcgc cttccagctg catgacgctg cgatcgacgt ccgcgcgcgc
     3901 ctggccgagc tgctccgtca tctgcgcgaa cgagcgcgtg aggccgccga tctcgtcgct
     3961 ggactcgtag acgggcttgg cgccgaggtc gcccgccgcg acctggcgca tgccctccgc
     4021 cagcagcagc agcggccgcg cgatctggtt gccgaagacg accgcgatca gcaccgagcc
     4081 gaacaccgac agcaccaccg tcagcgtcag cgtgccgaca tacatgcggc gcaggccctc
     4141 gcgcgcgagc gcccgctgct ggtagtcgcg ataggcggcc tgcacggcca gcgcgttgct
     4201 cgcgacgacc ggcggcagcg gctgcacggc gagcatgaag cgctcggtgg tgtcgagcga
     4261 catgccgctg gagggcacct gcaccagcgc ccgcacgcgc gcgagctggc cggtgccggg
     4321 cgaggcctcg tcctccaggc cttcgacctg gctcgagagc ccggtcgcgc gcgcctggcg
     4381 gatcagcacc gcggtcgggc ggtcgaccag cagcgcgggc cgcgagctcg ccgacatcag
     4441 gatctggccg ttgccgccga ccagcgcgag atcctgcacg ccgatctggt cgcgcaggcg
     4501 ctccagcgtg atcgcggcgc ccgacgagcg cgggtcgccc agccggccgg cggcgtagcg
     4561 cgccttgtcg acgaggtcgg acgacagcgt gtcgagcgtg cccttgccga ggttcaggcc
     4621 ggcgacgagg gcgttgtcga ccttggtgtc gaaccacgcg tcgatgctgc gcgtgacgaa
     4681 ctgcaggctc accgtgtaga tcagcgcgcc cggcaccacg cccacgagcg cgaagatggc
     4741 cgccagcttc agcagcagcc ggctgccgaa cttgccgctg tgccagcgga tcgccagccg
     4801 caccgccgcc gccgccagca ccagcagcag cagcatcgcg acgctgaagt tgagccagaa
     4861 cagccagacg aagttgcgct cgtacagcga aggctcgccg ccggacagcg agaccacgaa
     4921 cccgagcacc agcgcgacgc ccaggaaggc gatcagcgag accgcccagg tgaaccggcg
     4981 cgaggcgcgg ctcatcgggc ggccccgcgg cggacggccc ggcgggcggg cgcgacggcg
     5041 cgctggacgg cccg
//
LOCUS       DASVLN010000702        14299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_358297, whole genome shotgun sequence.
ACCESSION   DASVLN010000702 DASVLN010000000
VERSION     DASVLN010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14299
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358297"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VF453_16570"
     CDS             complement(<1..323)
                     /locus_tag="VF453_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HEX7689328.1"
                     /translation="MSLLKKPGWWRGRSAVIGIPYAWLLLFFLAPFLIVAKYSVSDMG
                     DIAVQDIATVADGAVKIVLKYSNFVALTDDRLYIVAYLTSLKYALCTTLLCLAIGYPF
                     AYFMAR"
     gene            complement(344..1444)
                     /gene="potA"
                     /locus_tag="VF453_16575"
     CDS             complement(344..1444)
                     /gene="potA"
                     /locus_tag="VF453_16575"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEX7689329.1"
                     /translation="MNNGADNAKFLQIQDVVKDFGGYMAVNHVSLDIAKGEIFALLGS
                     SGCGKTTLLRMLAGFETPTSGRIVLNGQDLAGMPPYERPLNMMFQSYALFPHLTVWDN
                     IAFGLRRDGMAKAHVAERVEAMLKLVQLSKFARRKPHQLSGGQQQRVALARSLAKQPQ
                     LLLLDEPLGALDKKLREETQIELVNIIEEVGVTCVMVTHDQEEAMTMASRIAVMSEGR
                     FLQVGTPGEVYETPASRFVADFIGNVNLMDGTLVEDEAGHCVVRCADCEHWIGHGITG
                     TLNMPVTVALRPEKIVLRRERPAEARNCVGGKIEEMSYFGATTVYRVRLASGLLLAVS
                     EANTARHTDNALTWGDIVWASWAPEAPVVLTQ"
     gene            complement(1570..2697)
                     /locus_tag="VF453_16580"
     CDS             complement(1570..2697)
                     /locus_tag="VF453_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7689330.1"
                     /translation="MRPTRSPVHAALALAAAALLALPPPARAQAPEEKVLNVYNWSDY
                     IGEDTIRGFEKETGIQVHYDAFDSNELLHAKLSAGRTGYDIVVPSSYWARMQIQGGLY
                     RKLDKAAIPNLANLDPAITAQMARIDPGNQYLVDWLWGYTTVGINVAKVKQALGATPM
                     PDNAWDLVFKPELVAKMKSCGVSFLDSGDEVVPAVMHYLGKPRGTVDNADYAQALGVL
                     RAVRPSVTLFSSSGYIDDLANGALCLVLGWSGDINIARQRAIDNRTGQDIEVLMPSTG
                     GLLFFDTMAIPKDAPHPGNATKWINYILRPDVEAGLTRKVFYANPVPASRKELAPRLA
                     DNATLFPGPEAMSRMVGPPDNWTNDLRRLQNRTFTAFKTGN"
     gene            complement(2712..4088)
                     /locus_tag="VF453_16585"
     CDS             complement(2712..4088)
                     /locus_tag="VF453_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7689331.1"
                     /translation="MTARTTRQWQEADSAHFLHPFTDHRSLAGKGARIVERAEGVYIW
                     DTEGHKILDGMSGLWCVNVGYGQQALIDAATRQMAQLPFYNAFFNTSTPPAIELAELL
                     AEVTPPQFRHVFFTSSGSEGNDTVVRMVRRYWDVLGQPQRQVIIGRWNGYHGSTMAGA
                     SMGGMKGMHEQGGLPIPGIEHIEQPFWYESGRALSPEAFGVQAARALERKIVEVGADK
                     VAAFIGEPVQGAGGVIVPPSTYWPEIQRICDQYGILLVSDEVICGFGRTGRWFGCETF
                     GFKPDLMTFAKGVTSGYVPLGGVMVGDRVADVLIERGGEFNHGYTYSGHPVACAVAVA
                     NLKLIRDLGLVDHVREEVGPHLARRFEELREHPLVGDVQTCGLMGAIQMVQDKATGKP
                     FPGDYALGMICRAHCFDNGLVMRAVGDRMIVAPPLVITRAQVDELADKAARCLDLTLA
                     DARARGWC"
     gene            complement(4123..5484)
                     /locus_tag="VF453_16590"
     CDS             complement(4123..5484)
                     /locus_tag="VF453_16590"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX7689332.1"
                     /translation="MANVSTFAELEQWLNTHRVTEVECLVPDLTGVARGKILPRQKFT
                     EDRGMRLPEAVVAMGVTGEFPSEGPYYDVITPTDRDMHLRPDPTTVRIVPWAADPTAQ
                     VLHDCYDPQGNLIPFAPRSVLRRVIDLYNAKGWDPVVAPELEFYLVARNTDPDMPLKP
                     PIGRSGRSETSRQAYSIDAVNEFDPLFEDVYDFCEKMELNVDTLIHEIGAGQMEINFF
                     HDAPLGLADEVFFFKRTVREAALRHDMFATFMAKPIAGEPGSAMHVHQSIVDRATGGN
                     LFSKPDGSASDEFYWYIGGLQRYIPAAMALFAPYVNSYRRLARHTAAPINIQWGTDNR
                     TVGIRSPVATPQARRVENRVIGADANPYVAIAATLACGYLGIVNRIAPTPECKGDAYL
                     GEYQLPRSLDDALRKLRAESDLAQVLGLDFVTVYTEIKDIEHAEFMKVISPWEREHLL
                     LHV"
     gene            complement(5591..6412)
                     /locus_tag="VF453_16595"
     CDS             complement(5591..6412)
                     /locus_tag="VF453_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX7689333.1"
                     /translation="MTSAVPALESSPNAPVNPSPAAARPVVLVPACNQMMGPHPYHVC
                     GKKYADAVRLAGGLPLVVPNADEVELEALLLLADGVFLTGSPSNVHPSHFDEDVLDLS
                     LPLDPARDAWTLPLIRRAIAMGLPLFTICRGTQEANVALGGSLHQAVHQVEGKRDHRA
                     DYAQPVGVQYGPAHEVLVEPGGVLERVVGAQRFDVNSVHGQAVHRLGAGLRVEARAPD
                     GVIEAFSVEEAPGFTLCVQWHPEWLAADNPVSMKMLTAFGTACRRYREGRRGPHP"
     gene            complement(6409..7701)
                     /locus_tag="VF453_16600"
     CDS             complement(6409..7701)
                     /locus_tag="VF453_16600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7689334.1"
                     /translation="MNFLQADRDLARNSYYAATTSRSQRHAPFSGAIRCDVAIVGGGL
                     AGLSAALDLRERGLDVVLLEARELGAGASGRNGGQAIHGLACGQDVIEAQLGLEDARR
                     VWDMSIEALDLLRERIGRHAIACDWRDGFLGVATSPKKARALDAWADGLERSYGYPLA
                     RIPAAELPRWIASPRYHGAIHDPRSGHLHPLKYCLGLADAAQRAGVRLFEGSEVLALE
                     HGRPVRLRTATGEVVADQALLAANVYLDGLAPRLSRRIMPVGTYIVCTEPLDRALADS
                     LVPTRSAVCDTDFVLDYFRTTDDDRMLFGGRVSYSTRTPRDLVESMRRRMVATFPQLA
                     GARIRHAWGGFVDITMNRAPDFGRLAANVYYLQGFSGHGLALTGLAGRLVAEAMTADA
                     GRFDLFARLRHRPFPGGDLLRTPALVLGMAWFRLKDLL"
     gene            complement(7834..9141)
                     /locus_tag="VF453_16605"
     CDS             complement(7834..9141)
                     /locus_tag="VF453_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7689335.1"
                     /translation="MRQDLDAYWMPFTANRQFKKAPRLFTKASGMHFWTDDGRQVLDG
                     IAGLWCVNAGHARPKIVQAIQAQAAEMDFCPPFQMAHPKAFELAAKVAEIAPAGMNRV
                     FFTNSGSESVETALKMAIAYHRVRGEGQRTRLIGRERGYHGVNFGGISVGGMVANRKM
                     FGTMLGGVDHIRHTHDPARNAFSVGQPTHGAELADDLERLVALHDGSTIAAVIVEPVA
                     GSTGVLVPPQGYLQRLREICDRHGILLIFDEVITGFGRTGQPFAAQTFGVTPDLMTCA
                     KGLTNGAVPMGAVIARQEIHDVFMTGPEHLIEFFHGYTYSAHPLACAAGLATLETYAD
                     EGLLTRAGEMAGYFADALHSLKGLPNVLDVRNIGLVGGVELAPIPGAPTKRAFDIFLD
                     CYERGALVRTTGDIVALSPPLIIERAQIDQLVGLLGDAIKRAA"
     gene            9295..9780
                     /locus_tag="VF453_16610"
     CDS             9295..9780
                     /locus_tag="VF453_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7689336.1"
                     /translation="MRQITKSPQLDAIDRSILAELQADGRLANVELAQRVHLSPSACL
                     RRVRQLEESGVIAGYVALLNPKLVGQSGTSFTIINLTTMSNQLLETFEAAVREVPEIL
                     DCYYVAGSNDYLIRFAYRDAEDLERFHTDVLMRLPGVERSNSMLVLRTVKKTTALPLA
                     G"
     gene            complement(9746..10264)
                     /locus_tag="VF453_16615"
     CDS             complement(9746..10264)
                     /locus_tag="VF453_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689337.1"
                     /translation="MIPQIAFFASVGFGLVAWSVLAALFAWPRLCDRPRADALRPLLV
                     IHAFRFVGLSFLVPGVVGADLPQAFARGAAGGDLVAAVLALLALATQRRRIGTPLAWA
                     WCAWGSVDLVNAFYQAGTNGLRPSQLGAAYYLPTFVVPMLLVTHVLALRILLRPDAAG
                     APIRPAAARSSS"
     gene            10416..11327
                     /locus_tag="VF453_16620"
     CDS             10416..11327
                     /locus_tag="VF453_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7689338.1"
                     /translation="MKRDYTIERGTLDGVEAFVRVAERRSFRQAAADLGVTPSAVSQT
                     IRTLEMRVGVALFTRTTRSVGLTEAGERFLEHARPAVEAIVAATDAARGLGARPSGLL
                     RLAVPRSVVPLVLQPLLASFCAAYPEVVVEIAASEELVDLAKGGYDAGIRMGEFIAED
                     MVAIRLTPSFPFVVVGSPAYLARHGRPAAPADLARHACVRLRRSSGAIAPWRLLDGDG
                     PIDLAVSGPLVVNDYPTMLAAALDGLALAQVPRPVASEALASGRLEELLAGHAPSSPG
                     LFLYFPSRAQVLPKLRAFIDHFGSSAR"
     gene            complement(11348..11878)
                     /locus_tag="VF453_16625"
     CDS             complement(11348..11878)
                     /locus_tag="VF453_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7689339.1"
                     /translation="MTEPRSTDTPTAGAAPVLDRVDATHAQMRYWLGELQTLVNHMED
                     RGWDTWARDHARSVIDFFSNTARQHHADVDRQVCPLVQGRGDDFLDSHIDRLRQDQGW
                     LEENWREVLAQLQPVAEGFGGYDVDMLRHASDVYAALLLEHLALEQGTVFPAARASAD
                     GLLQSAEQRRSESAAA"
     gene            12295..13584
                     /gene="aceA"
                     /locus_tag="VF453_16630"
     CDS             12295..13584
                     /gene="aceA"
                     /locus_tag="VF453_16630"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX7689340.1"
                     /translation="MTREQQIAALEKDWAENPRWKGIQRGYTAADVVRLRGSLQIEHT
                     LARRGAEKLWNLVNTEPFVNALGALTGNQAMQQVKAGLKAIYLSGWQVAGDANSNGEM
                     YPDQSLYSVDSVPKVVKKINATFKRADEIQWSEGKDDTDFFAPIVADAEAGFGGVLNA
                     FELMKAMIDAGAAGVHFEDQLAAAKKCGHMGGKVLVPTREAVAKLSAARLAADVMGTP
                     TVLLARTDAEAGDLVTSDIDDNDKPFCTGERTVEGFFRTRNGLEQAISRGLAYAPYAD
                     MIWCETGKPDLAFAKAFADAIHAKFPGKLLAYNCSPSFNWKKNLDDATIAKFQRELGA
                     MGYKFQFITLAGFHALNYSMFNLAYGYARNNMSAFVELQEAEFAAAEKGFTAVKHQRE
                     VGTGYFDAITTTIEREASTGALKGSTEDEQFFDKKHA"
     gene            13735..14208
                     /locus_tag="VF453_16635"
     CDS             13735..14208
                     /locus_tag="VF453_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEX7689341.1"
                     /translation="MTTSVPTLYDWLGGIDALERLTARFYERVAQDALLAPVFAHMGA
                     DHPRHVAAFLGEVLGGPKTYSAEHGGHPHMVRQHLGRHLAQDQRRRWVELLLSTADEL
                     RMPDDPEFRSALVGYLEWGSRLAVINSQPGAAVDPGAAMPSWGWGEVKGPYTGGS"
BASE COUNT         2124 a         4793 c         5215 g         2167 t
ORIGIN      
        1 cgcgccatga aatacgcgaa cgggtatccg atcgcgaggc acagcagcgt cgtgcacagc
       61 gcgtacttca ggctcgtcag gtaggcgacg atgtagagcc tgtcgtcggt cagcgcgacg
      121 aagttcgagt acttcaggac gatcttcacc gcgccgtcgg cgacggtcgc gatgtcctgc
      181 accgcgatgt cgcccatgtc cgagaccgag tacttggcga cgatcaggaa cggcgcgagg
      241 aagaacagca gcagccacgc ataggggatg ccgatgacgg cgctgcgtcc gcgccaccag
      301 ccgggcttct tgagcaagct catctcgatt cccgcctcca tcctcattgc gtcagcacga
      361 ccggcgcttc cggtgcccag ctcgcccaga cgatgtcgcc ccaggtcagg gcgttgtcgg
      421 tgtggcgcgc ggtgttggcc tcgctgacgg ccagcagcag gccgctggcc aggcgcacgc
      481 ggtagaccgt cgtcgcgccg aagtacgaca tctcctcgat cttgccgccc acgcagttgc
      541 gcgcctcggc cgggcgttcg cggcgcagca cgatcttctc gggccgcagc gccacggtca
      601 cgggcatgtt gagcgtgccg gtgatgccat ggccgatcca gtgctcgcag tcggcgcagc
      661 gcacgacgca atggccggct tcgtcctcga cgagcgtgcc gtccatcagg ttgacgttgc
      721 cgatgaagtc ggcgacgaag cgcgaggccg gcgtctcgta gacctcgccc ggcgtaccca
      781 cctgcaggaa gcggccttcg ctcatcaccg cgatgcggct ggccatggtc atggcctctt
      841 cctggtcgtg cgtcaccatc acgcaggtca cgccgacctc ctcgatgatg ttgaccagct
      901 cgatctgcgt ctcctcgcgc agcttcttgt ccagcgcgcc cagcggctcg tccagcagca
      961 gcagctgcgg ctgcttggcc aggctgcgcg cgagcgccac gcgctgctgc tggccgcccg
     1021 agagctggtg cggcttcctg cgcgcgaact tcgacagctg caccagcttg agcatcgcct
     1081 ccacgcgctc ggcgacgtgc gccttggcca tgccgtcgcg gcgcaggccg aaggcgatgt
     1141 tgtcccagac cgtcaggtgc gggaacagcg cgtaggactg gaacatcatg ttcagcggcc
     1201 gctcgtaggg cggcatgccg gcgagatcct ggccgttcag gacgatgcgg cccgacgtgg
     1261 gcgtctcgaa acccgccagc atgcgcagca gcgtcgtctt gccgcagccg gaggatccga
     1321 gcagggcgaa gatctcgccc ttggcgatgt ccaggctcac gtggttgacg gccatgtagc
     1381 cgccgaaatc cttgaccacg tcctggatct ggagaaattt tgcgttgtcg gcgccgttgt
     1441 tcatgaggtc gtgccgccgg cggcggcttg tcgggtcgga cggttgggag acggcggccg
     1501 cacgcgcggc gctcggcccg ccggggccgt attgtcgtcg cagcgcaggc gcggcggccg
     1561 gaccgtctct cagttgccgg tcttgaaggc ggtgaaggtg cggttctgaa gtcggcggag
     1621 gtcgttggtc cagttgtccg gcgggccgac catgcggctc atggcctcgg ggccgggaaa
     1681 gagggtggcg ttgtcggcca ggcgcggggc gagctccttg cgcgaggccg gcaccgggtt
     1741 ggcgtagaag accttgcgcg tcaggccggc ctcgacgtcg ggccgcagga tgtagttgat
     1801 ccacttggtc gcgttgcccg ggtgcggcgc gtccttcggg atggccatcg tgtcgaagaa
     1861 cagcaggccg ccggtgctgg gcatcagcac ctcgatgtcc tggcccgtcc tgttgtcgat
     1921 cgcgcgctgg cgggcgatgt tgatgtcgcc cgaccagccc agcaccaggc acagcgcgcc
     1981 gttggcgagg tcgtcgatgt agcccgagga gctgaagagg gtcaccgacg gccgcaccgc
     2041 gcgcagcacg cccagcgcct gcgcgtagtc ggcgttgtcg acggtgccgc gcggcttgcc
     2101 gaggtagtgc atgaccgcgg gcaccacctc gtcgccggag tcgaggaagg acacgccgca
     2161 cgacttcatc ttcgcgacca gctcgggctt gaagacgaga tcccaggcgt tgtcgggcat
     2221 cggcgtcgcg cccagcgcct gcttcacctt ggcgacgttg atgccgacgg tggtgtagcc
     2281 ccacagccag tcgacgaggt actggttgcc gggatcgatc cgcgccatct gcgcggtgat
     2341 ggccgggtcg aggttggcca ggttggggat cgccgccttg tccagcttgc ggtacaggcc
     2401 gccctggatc tgcatgcgcg cccagtacga ggacggcacg acgatgtcgt agccggtgcg
     2461 gccggcgctg agcttggcgt gcagcagctc gttgctgtcg aaggcgtcgt agtggacctg
     2521 gatgccggtc tccttctcga agccgcggat cgtgtcctcg ccgatgtagt ccgaccagtt
     2581 gtagacgttg agcaccttct cctcgggcgc ctgcgcgcgc gccggcgggg gcagggccag
     2641 gagcgcggcg gcggccagcg cgagcgcggc gtgcacgggg gacctggtcg gacgcatggc
     2701 gcgggacggc ctcagcacca gccgcgggcg cgggcgtccg cgagcgtgag gtcgaggcac
     2761 ctggccgcct tgtcggcgag ctcgtcgacc tgcgcgcggg tgatgaccag cggcggcgcg
     2821 acgatcatgc ggtcgccgac cgcgcgcatc acgaggccgt tgtcgaagca gtgcgcgcgg
     2881 cagatcatgc ccagcgcgta gtcgccgggg aagggcttgc cggtcgcctt gtcctgcacc
     2941 atctggatcg ctcccatgag gccgcacgtc tgcacgtcgc ccaccagcgg gtgctcgcgc
     3001 agttcctcga aacgccttgc aagatgcggg ccgacttcct cgcgcacgtg gtcgacgagc
     3061 cccaggtcgc ggatcagctt caggttggcg acggctaccg cgcaagccac gggatgcccg
     3121 gagtaggtat agccgtggtt gaactcgccg ccgcgctcga tcagcacgtc ggcgacgcga
     3181 tcgcccacca tcacgccgcc cagcggcacg tagccggacg tcacgccctt ggcgaaggtc
     3241 atgaggtcgg gcttgaagcc gaacgtctcg cacccgaacc agcggccggt gcggccgaag
     3301 ccgcagatca cctcgtcgct cacgagcagg atgccgtact ggtcgcagat gcgctggatc
     3361 tcgggccagt aggtcgacgg cgggacgatg acgccgcccg cgccctgcac gggctcgccg
     3421 atgaaggcgg ccaccttgtc ggcgccgacc tcgacgatct tgcgttccag ggcccgcgcc
     3481 gcctgcacgc cgaacgcctc gggcgagagc gcgcgccccg attcgtacca gaacggctgc
     3541 tcgatgtgct cgatgccggg gatcggcagg ccgccctgtt cgtgcatccc cttcatgccg
     3601 cccatcgacg cgccggccat cgtcgagccg tggtagccgt tccagcgccc gatgatcacc
     3661 tggcgctgcg gctggccgag gacgtcccag tagcggcgca ccatgcgcac gacggtgtcg
     3721 ttgccctcgg agccggagct cgtgaagaag acgtgcctga actgcggcgg cgtgacctcg
     3781 gcgagcagtt ccgcgagctc gatggccggc ggcgtcgagg tgttgaagaa cgcgttgtag
     3841 aagggcagct gcgccatctg ccgcgtcgcc gcgtcgatca gcgcctgctg cccgtagccg
     3901 acgttcacgc accacagccc ggacatgccg tcgaggatct tgtggccctc ggtgtcccag
     3961 atgtagacgc cctcggcccg ctcgacgatg cgcgcgccct tgccggcgag cgagcggtgg
     4021 tcggtgaacg ggtgcaggaa gtgggcggag tcggcctcct gccattgcct cgtggtgcgt
     4081 gcggtcatcg tgtcggctcc cgtggttcgt gtggcgagat ggtcagacgt gcagcagcag
     4141 gtgctcgcgc tcccagggcg aaatcacctt catgaactcg gcgtgctcga tgtccttgat
     4201 ctccgtgtag acggtgacga aatccaggcc gagcacctgg gcgaggtccg actcggcgcg
     4261 cagcttgcgc agcgcgtcgt cgaggctgcg gggcagctgg tactcgccga ggtaggcgtc
     4321 gcccttgcac tccggcgtgg gcgcgatgcg gttgacgatg ccgaggtagc cgcaggcgag
     4381 cgtcgcggcg atcgcgacgt aggggttggc atcggcgccg atgacgcggt tctcgacccg
     4441 gcgcgcctgc ggcgtggcca ctggcgagcg gatgcccacg gtacggttgt ccgtgcccca
     4501 ctggatgttg atcggcgcgg ccgtgtggcg cgccaggcgg cggtaggagt tgacgtaggg
     4561 tgcgaacagg gccatcgccg ccgggatgta gcgctgcagg ccgccgatgt accagtagaa
     4621 ctcgtcgctc gcgctgccat ccggcttgct gaagaggttg ccgccggtgg cgcggtcgac
     4681 gatgctctgg tgcacgtgca tggcgctgcc cggctcgccg gcgatcggct tggccatgaa
     4741 ggtggcgaac atgtcgtggc gcagcgcggc ctcgcgcacc gtgcgcttga agaagaagac
     4801 ctcgtcggcg agcccgagcg gggcgtcgtg gaagaagttg atctccatct ggccggcgcc
     4861 gatctcgtgg atcagcgtgt cgacgttgag ctccatcttc tcgcagaagt cgtacacgtc
     4921 ctcgaacagc gggtcgaact cgttgaccgc gtcgatcgag taggcctggc gcgaggtctc
     4981 ggagcgcccg ctgcggccga tcggcggctt caggggcatg tcggggtcgg tgttgcgggc
     5041 gacgagatag aactcgagct ccggcgcgac gaccggatcc cagcccttgg cgttgtagag
     5101 gtcgatcacg cgccgcagca ccgagcgcgg cgcgaacggg atcaggttgc cctgcgggtc
     5161 gtagcagtcg tggagcacct gcgcggtcgg gtcggcggcc cacggcacga tgcgcaccgt
     5221 cgtggggtcg ggccgcaggt gcatgtcgcg gtcggtgggc gtgatgacgt cgtagtacgg
     5281 gccctcggac gggaactcgc ccgtcacgcc catcgcgacg accgcctccg gcagccgcat
     5341 gccgcggtcc tcggtgaact tctggcgcgg caggatcttg ccgcgggcga cgccggtcag
     5401 gtcgggcacg aggcactcga cctcggtgac gcggtgcgtg ttgagccact gttcgagctc
     5461 tgcgaaggtg gaaacgttgg ccatgggatc tttcgtggag gtctgggctg cgcggacggc
     5521 gaggacgcat gcatcggccg tgccatgcag ggcggtcggg cccgcgcgga cgcgggcgcg
     5581 gccgtgcgcc tcacgggtgg gggccgcggc gtccctcgcg atagcggcgg caggccgtgc
     5641 cgaaggccgt gagcatcttc atcgagaccg ggttgtcggc cgcgagccac tccggatgcc
     5701 actgcacgca cagcgtgaag cccggggcct cctcgaccga gaacgcctcg atcacgccgt
     5761 cgggcgcgcg cgcctcgacg cgcaaccccg cgccgagccg gtggacggcc tggccgtgca
     5821 ccgagttgac gtcgaagcgc tgcgcgccga cgactcgctc gaggacgccg cccggctcga
     5881 ccagcacctc gtgcgccggc ccgtactgca cgcccaccgg ttgtgcgtag tcggcgcggt
     5941 ggtcgcgctt gccctcgacc tggtgcaccg cctggtgcag cgagccgccg agcgccacgt
     6001 tggcttcctg cgtgccgcgg cagatggtga acagcggcag gcccatcgcg atggcgcggc
     6061 ggatcagcgg cagcgtccac gcgtcgcgcg cgggatccag cggcagcgac aggtcgagca
     6121 cgtcctcgtc gaagtgcgag gggtggacgt tgctcggcga gccggtgagg aagacgccgt
     6181 cggccagcag gagcagggcc tcgagttcga cctcgtcggc attgggcacg accagcggca
     6241 gcccgccggc caggcgcacg gcgtcggcgt acttcttccc gcagacgtgg tacgggtgcg
     6301 ggcccatcat ctggttgcag gcgggcacga gcacgaccgg ccgcgcggcc gcaggcgacg
     6361 ggttcaccgg cgcgttgggc gaggattcga gggcggggac ggcggaagtc atagcagatc
     6421 cttgagtcga aaccaggcca tgccgagcac cagcgcgggc gtgcgcagca ggtcgccgcc
     6481 ggggaacggc cggtgccgca gccgtgcgaa caggtcgaag cgtccggcat cggcggtcat
     6541 cgcctcggcc acgaggcggc ccgcgaggcc ggtcagcgcg agcccgtggc ccgagaagcc
     6601 ctgcaggtag tacacgttcg cggcgaggcg cccgaagtcg ggggcgcggt tcatcgtgat
     6661 gtcgacgaag ccgccccagg catggcggat tctcgcgccg gcgagctgtg gaaacgtcgc
     6721 gaccatgcgc cggcgcatgg attcgaccag gtcgcgcggc gtgcgcgtgc tgtagctgac
     6781 gcggccgccg aacagcatgc ggtcatcgtc ggtggtgcgg aagtagtcga gcacgaagtc
     6841 ggtgtcgcac accgccgagc gcgtcggcac gagcgagtcg gcgagcgcgc gatccagcgg
     6901 ctcggtgcag acgatgtagg tgccgacggg catgatgcgg cgcgagagcc ggggcgcaag
     6961 cccgtccagg tagacgttgg ccgcgagcag cgcctggtcg gcgacgacct ccccggtggc
     7021 ggtgcgcagc cgcacgggcc ggccatgctc gagcgcgagc acctcgctgc cctcgaacag
     7081 ccgcacgccc gcgcgctgcg ccgcgtcggc caggccgagg cagtacttca gcggatgcag
     7141 gtggccggag cgggggtcgt ggatcgcgcc gtggtagcgc gggctggcga tccagcgcgg
     7201 cagctcggcc gcgggaatgc gcgcgagcgg gtagccgtag ctgcgctcga ggccgtcggc
     7261 ccaggcgtcg agcgcgcgcg cctttttcgg gctcgtggcg acgcccagga agccgtcgcg
     7321 ccagtcgcag gcgatcgcgt ggcggccgat gcgctcgcgc agcaggtcga gcgcctcgat
     7381 ggacatgtcc cagacgcggc gcgcgtcctc gaggcccagc tgcgcctcga tcacgtcctg
     7441 cccgcaggcg agcccgtgga tcgcctggcc gccgttgcgg ccgctggcgc cggcgccgag
     7501 ctcgcgcgcc tcgagcaaga cgacgtccag cccgcgctcg cgcaggtcga gcgcggcgct
     7561 gaggccggcg aggccgccgc cgacgatcgc gacgtcgcag cggattgcac ccgagaaggg
     7621 cgcatgccgc tgcgaccggg acgtcgtcgc tgcgtagtac gaattccgcg cgagatcgcg
     7681 gtccgcttgc aggaagttca tgagctcgat gcgctaggca ggccttggtt ctggcgccgg
     7741 cggcatgccg gcaggggtat tggacaccga agcgaacgga agaatcgatc ggacaagccc
     7801 ggaaagcggg cgccgcgcgg cgcccggcgt ccctcaggcg gcgcgcttga tcgcgtcgcc
     7861 gagcaggccg accagctggt cgatctgcgc ccgctcgatg atcagcggcg gcgacagcgc
     7921 cacgatgtcg cccgtcgtgc gcaccagcgc gccgcgctcg tagcagtcga ggaagatgtc
     7981 gaacgcgcgc ttggtgggcg cgcccgggat gggcgcgagc tccacgccgc cgaccagtcc
     8041 gatgttgcgc acgtcgagca cgttgggcag gcccttgagc gagtgcagcg cgtccgcgaa
     8101 gtagcccgcc atctcgccgg cgcgcgtgag caggccctcg tcggcgtagg tctccagcgt
     8161 cgccaggccg gcggcgcagg cgagcgggtg cgccgagtag gtgtagccgt ggaagaactc
     8221 gatcaggtgc tcggggccgg tcatgaagac gtcgtggatc tcctggcgcg cgatcaccgc
     8281 gcccatcggc acggcgccgt tggtcaggcc cttggcgcag gtcatgaggt cgggcgtgac
     8341 gccgaaggtc tgcgccgcga agggctggcc ggtgcggccg aagccggtga tgacctcgtc
     8401 gaagatcagc aggatgccgt gccggtcgca gatctcgcgc agccgctgca ggtagccttg
     8461 cggcgggacc agcacgcccg tcgaaccggc gaccggctcg acgatcaccg cggcgatggt
     8521 ggagccgtcg tgcagcgcga ccaggcgctc gaggtcgtcg gcgagctccg cgccgtgcgt
     8581 aggctggccg acgctgaagg cgttgcgcgc cgggtcgtgg gtgtggcgga tgtggtcgac
     8641 gccgcccagc atcgtgccga acatcttgcg gttggcgacc atgccgccca ccgagatgcc
     8701 gccgaaattg acgccgtggt agccgcgctc gcgaccgatg aggcgcgtgc gctggccctc
     8761 gccgcgcacg cggtggtagg cgatcgccat cttcagcgcg gtctcgaccg actccgagcc
     8821 cgagttggtg aagaagacgc ggttcatgcc ggcgggcgcg atctccgcga ccttggccgc
     8881 gagctcgaag gccttgggat gcgccatctg gaacggcggg cagaagtcca tctcggcggc
     8941 ctgcgcctgg atggcctgca cgatcttcgg gcgcgcatgg cccgcgttca cgcaccacag
     9001 gccggcgatg ccgtcgagca cctggcggcc gtcgtcggtc cagaagtgca tgccggacgc
     9061 cttcgtgaac agccgcggcg ccttcttgaa ctggcggttc gcggtgaagg gcatccagta
     9121 ggcgtcgagg tcttggcgca tcggcgggct ttcgcgggga agtcggtgcg ggcgagcccg
     9181 cgggcatgcc gtccactgta gcggcgcggc gacgcaatgt gcacgcgcgt tcgcacaggg
     9241 ttcacccttg gaaacgcaat atcatgcgaa gaactgccga gctgcccgca cgccatgcgc
     9301 caaatcacga aatccccgca attggatgcg atcgaccgca gcatcctggc cgagctccag
     9361 gcggacggcc gcctggccaa cgtcgagctc gcgcagcgcg tccacctgtc gccgtccgca
     9421 tgcctgcggc gggtcaggca gctcgaggag tccggcgtca tcgcgggcta tgtcgcgctg
     9481 ctgaacccga agctcgtcgg ccagagcggc acgagcttca ccatcatcaa cctgacgacg
     9541 atgagcaacc agctgctgga gaccttcgag gcggcggtgc gcgaggtgcc cgagatcctc
     9601 gactgctact acgtcgccgg ctcgaacgac tacctgatcc gcttcgccta ccgcgacgcc
     9661 gaggatctcg agcgcttcca caccgacgtg ctgatgcgcc tgccgggcgt ggagcgttcc
     9721 aactcgatgc tggtgctgcg cacggtcaag aagacgaccg cgctgccgct ggccggatag
     9781 gcgcgcccgc ggcatccggc cgcaacagga tgcgcagcgc caggacgtgc gtgacgagca
     9841 gcatcggcac cacgaaggtg gggaggtagt acgcggcgcc cagctgcgag ggccgcagcc
     9901 cgttcgtgcc cgcctggtag aaggcgttca ccaggtccac gctgccccag gcgcaccagg
     9961 cccaggcgag cggcgtcccg atccggcggc gctgggtggc cagcgcgagc agcgccagca
    10021 ccgccgcgac caggtcgccg cccgcggcgc cgcgggcgaa cgcctgcggc aggtcggcgc
    10081 cgacgacgcc gggaacgagg aaggacaggc cgacgaagcg gaacgcgtgg atgacgagca
    10141 gcgggcgcag cgcgtccgcc cgcggccggt cgcacaggcg cggccaggcg aacagcgccg
    10201 ccaggacgct ccaggcaaca aggccgaagc ctacactcgc gaagaaggcg atctgcggga
    10261 tcatggcggc ctccttcaga agaggattcg gaggccggcg gccgcgttga agaggatcga
    10321 gggtcgggtc atggcgcgtc tcctgcgagg cgagaggatg gccggactct agggcgcttg
    10381 cggtgttcga taaactggcc tagtctgcgc gaaccatgaa gcgcgactac acgatcgagc
    10441 gcgggacgct ggatggcgtg gaggccttcg tccgggtggc cgagcgtcgc agcttccgcc
    10501 aggccgccgc cgacctcggg gtcacgccat cggcagtgag ccagacgata cggacgctgg
    10561 agatgcgggt cggggtcgcg ctgttcacgc gcacgacccg cagcgtcggc ctcacggagg
    10621 cgggcgagcg cttcctggag cacgcacgtc ccgcggtgga ggcgatcgtt gccgcgaccg
    10681 acgcggcgcg cggcttgggc gcgcggccgt ccggcctgtt gcgcctggcg gtcccgcgtt
    10741 cggtggtgcc gctcgtgctg cagccgctgc tggcgtcgtt ctgcgccgcg tatccggagg
    10801 tggtcgtgga gatcgccgcg agcgaggagc tggtcgacct cgccaagggc ggctacgacg
    10861 ccggcatccg gatgggcgag ttcatcgccg aggacatggt cgcgatccgg ctgacgccgt
    10921 cgtttccctt cgtggtggtc ggcagtccgg cctacctggc gcgccacgga cggccggcgg
    10981 cgcccgccga tctcgcgcgg cacgcgtgcg tgcggctgcg ccgctcgagc ggggcgatcg
    11041 cgccctggcg gctgctcgat ggcgacgggc cgatcgacct tgcggtgtcg ggcccgctgg
    11101 tggtcaacga ctacccgacg atgctggcgg cggcgctcga cgggctcgcg ctcgcccagg
    11161 tgccacggcc ggtcgcgagc gaggcgctcg cgtcaggccg gctcgaggaa ctgctcgccg
    11221 ggcacgcgcc gtcgtcgccc gggctgttcc tctactttcc gagccgggca caggtgctgc
    11281 ccaagctgcg tgccttcatc gaccacttcg gctcgtcggc gcgctgacga gaggtgcgcg
    11341 cgccgcgtca ggccgcggcg ctttcgctgc ggcgctgctc cgcgctttgc agcagcccgt
    11401 cggcggaggc gcgcgccgcc gggaacacgg tgccctgctc gagggccagg tgctccagca
    11461 gcagcgcggc gtagacgtcg ctcgcgtggc gcagcatgtc gacgtcgtag ccgccgaagc
    11521 cctcggcgac gggctggagc tgggccagga cctcgcgcca gttctcctcg agccagccct
    11581 ggtcctggcg caggcggtcg atgtgggagt cgaggaagtc gtcgccgcgg ccctgcacca
    11641 gcgggcacac ctggcgatcg acgtccgcgt ggtgctggcg ggcggtgttg gagaagaagt
    11701 cgatcaccga gcgcgcgtgg tcgcgggccc aggtgtccca gccgcgatcc tccatgtggt
    11761 tgaccagggt ctggagctcg ccgagccagt agcgcatctg cgcgtgggtg gcgtcgacac
    11821 ggtcgagcac cggcgcggcg ccggcggtgg gggtgtcggt cgagcggggt tcggtcacgg
    11881 cagctcctcg ggttggacgt cgggggtggg cctcagcatc gcatgtccgg cgggcttcgc
    11941 gcaaggcgcc accacgccct ctcggtgtat tccagggctc gtgctggcga aggccgttcg
    12001 cgtcatcgaa acgtcgtctt cgcgtgacac ggcgtgcatt tcgcatcgcc gagggccttt
    12061 cacattgcgg gaagaaatcg atgtgcagcg cagcaagatt tctcattatg agaattgata
    12121 tgccacatag cgaaaatagc ttcataaggc attgattcaa aagactaaga atcttggtct
    12181 tgtataagac ataggtcttc cctttcttgc gggaagtgag tacgctggat cccaacgaac
    12241 gactcttttc tcctcttcga ctcaaccgca agaaaggcac ccaccatgac ccgactgacc
    12301 cgcgaacagc agatcgccgc cctcgagaag gactgggccg agaacccgcg ctggaagggt
    12361 atccagcgcg gctacacggc cgccgatgtc gtgcgcctgc gcggctcgct gcagatcgag
    12421 cacacgctgg cccggcgcgg cgccgagaag ctgtggaacc tcgtgaacac cgagcccttc
    12481 gtcaatgcgc tgggcgcgct cacgggcaac caggcgatgc agcaggtcaa ggccggcctg
    12541 aaggcgatct atctctccgg ctggcaggtc gccggcgacg ccaacagcaa cggcgagatg
    12601 tatcccgacc agtcgctgta ctcggtggac tcggtgccca aggtcgtcaa gaagatcaac
    12661 gcgacgttca agcgcgccga cgagatccag tggtcggaag gcaaggacga caccgacttc
    12721 ttcgcgccca tcgtggccga cgcggaagcc ggcttcggcg gcgtgctgaa cgcgttcgag
    12781 ctgatgaagg ccatgatcga cgcgggcgcg gccggcgtgc acttcgagga ccagctcgcc
    12841 gccgccaaga agtgcggcca catgggcggc aaggtgctcg tgccgacccg cgaggcggtc
    12901 gccaagctgt ccgccgcgcg cctggccgcg gacgtgatgg gcacgcccac cgtgctgctg
    12961 gcgcgcaccg acgccgaggc cggcgatctc gtgacgagcg acatcgacga caacgacaag
    13021 cccttctgca ccggcgagcg caccgtcgaa ggcttcttcc gcacccgcaa cggcctcgag
    13081 caggcgatca gccgcggcct cgcctacgcg ccgtacgccg acatgatctg gtgcgagacg
    13141 ggcaagcccg acctcgcgtt cgccaaggcc ttcgccgacg cgatccacgc caagttcccg
    13201 ggcaagctgc tggcttacaa ctgctcgccg tcgttcaact ggaagaagaa cctcgacgac
    13261 gccacgatcg ccaagttcca gcgcgagctg ggcgcgatgg gctacaagtt ccagttcatc
    13321 acgctggccg gcttccacgc gctgaactac tcgatgttca acctcgccta cggctacgcg
    13381 cgcaacaaca tgagcgcctt cgtcgagctg caggaagccg agttcgccgc cgcggagaag
    13441 ggcttcaccg cggtcaagca ccagcgcgag gtcggcaccg gctacttcga cgcgatcacg
    13501 acgaccatcg agcgcgaggc ctcgactggc gcgctcaagg gctcgaccga ggacgagcag
    13561 ttcttcgaca agaagcacgc gtgacgctgc gcgcgtgacg tcgccccggc cgtcgggttt
    13621 ccggccggcg gcagcgatga ggcccgtccg acttgagcac cggacggttt tttttcgtca
    13681 tagactgcgc tgcgtcgcga cggcgccccg gtcgtcgcct ccggagattc cgcgatgacc
    13741 acgtccgtcc cgaccctgta cgactggctc gggggcatcg acgcgctcga gcgcctgacc
    13801 gcgcgcttct acgagcgcgt cgcgcaggat gcgctgctcg cgccggtgtt cgcgcacatg
    13861 ggcgccgacc acccgcgcca cgtcgcggcc ttcctgggcg aggtgctggg cggcccgaag
    13921 acttattcgg cggagcacgg cgggcacccg cacatggtgc gccagcacct gggccgccac
    13981 ctcgcgcagg accagcgacg ccgctgggtc gagctgctgc tgtcgaccgc cgacgaactc
    14041 cgcatgcccg acgatcccga gttccgctcg gcgctggtgg gctacctcga atggggctcg
    14101 cggctcgcgg tcatcaactc gcagcccggt gccgcggtcg acccgggcgc ggccatgccg
    14161 agctggggct ggggcgaggt gaaggggccg tacaccggcg gctcatgacg ggtcgccgcc
    14221 gccgggtacg cgctcagcgc agcggcggcg cgccggggat gccgtcgcgc gcgatcgcgc
    14281 gcgcgttggc ggccatcga
//
LOCUS       DASVLN010000703         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_521333, whole genome shotgun sequence.
ACCESSION   DASVLN010000703 DASVLN010000000
VERSION     DASVLN010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521333"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF453_16640"
     CDS             <1..193
                     /locus_tag="VF453_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7689342.1"
                     /translation="GRTYDYVVALRAVQTSDFMTADWAELPYGLLKKVSGRIINEVRG
                     INRVTYDVSSKPPATIEWE"
     gene            671..1342
                     /locus_tag="VF453_16645"
     CDS             671..1342
                     /locus_tag="VF453_16645"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEX7689343.1"
                     /translation="MPAQLPLFLDVPPPGPARPALQPVPLEPWADPWPRAPEGDGAAL
                     LHSLFFALAPAPDDARRLRAEGLALDARFAIGGAPLESHRLHVSLFGVGAYLRTRPEA
                     DVERWRRAAAAVGCAAFEVVFDRIATFGGAGRSLVLKAADESALAGLQALYESLGIAL
                     ANAGEHVTQRRITPHMSLSYHGRPMPETAIEPVRWRAHELVLIDSHIGLHHHEVLGRW
                     RLQDG"
     gene            complement(1446..2405)
                     /locus_tag="VF453_16650"
     CDS             complement(1446..2405)
                     /locus_tag="VF453_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7689344.1"
                     /translation="MSERLSGIDVFVAAVEAGTFAGAALRMGLTRSAVAKSVGRLETR
                     LGTRLFRRTTRSQSLTDDGQAYYERCRRALAELEAADAVFEAGRREPAGRLRLTMPEL
                     IGRRCVAPLLLALGREHAGLSFEVSFDDRRVDLVEDRIDLAIRSGPLADSSSVAARAL
                     GHQWVCVVASPDYFARHGRPSCFEEMAARRDAHAFIGYARQGAPKPWLHRDGRGAPAE
                     FDPRARFVFDSLEVVADAARGGLGLARVPHWLAEPEFAAGRLVQVFEEPHPYGYELHA
                     VWPHARALPLKVRVLVDLLVQQLLPRMQPPRPIPQAPGGPLLG"
     gene            2507..>2940
                     /locus_tag="VF453_16655"
     CDS             2507..>2940
                     /locus_tag="VF453_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7689345.1"
                     /translation="MTDTLRQWRFVDNGLAHLKLHETVLPEPGPGQILVRIEAASLNY
                     RDLMLAEGRYLPTTLPYVPGSDLAGVVQAVGPGVRRWKGGERVIGTYAAGWLDGAAPA
                     DVVPLGFPGPGALASHVVLDEQWAVAAPQSLTAAEASTLPIAG"
BASE COUNT          394 a         1041 c         1121 g          384 t
ORIGIN      
        1 cggccgcacc tacgactacg tggtcgcgct gcgcgccgtg cagaccagcg acttcatgac
       61 cgccgactgg gccgagctgc cctacggcct gctgaagaag gtctcgggcc gcatcatcaa
      121 cgaggtgcgc ggcatcaacc gggtgaccta cgacgtctcg agcaagccgc cggcgacgat
      181 cgagtgggag tgagctggat ggccgcgcgt cggcgtgcct ccgcatgcct cgcggcggtg
      241 ccggcgctcc tgctcgcggc cgcctgcgac cgcggcgcgc ggctggcagt ggcggcgggc
      301 gccgccttga cgagccgcgt cgccgcggca aggtctcgat cgtcccgctc gacgctggcc
      361 accccgcggc gttcgtcgag aacggcagct gctggcaggg cgcctgcgga gacgacctga
      421 cgacgatcga gctcaccacc gcgggcggat ggacgctcgg cgacgcggtg gcgctcagtt
      481 cggaggccgt acgctgccgt tcggcgagga cagggcgggc gcatgctcac cggacgcttg
      541 cgccgacgcc ggggtggacg gcgtcggcgg atggggccgg caggcgcgga cttgacggca
      601 tccggaggct gtacgaacat acagtccata cgggatcgcc gcatccctgc tgaccgccga
      661 aggttcgacc atgcctgcgc aattgccgct gtttctcgat gtcccgccgc ccggcccggc
      721 gcggccggcg ctgcagcccg tcccgctgga gccgtgggcg gacccctggc cgcgggcgcc
      781 ggagggagac ggcgcggccc tgctgcattc gctgttcttc gcgctcgcgc cggcgccgga
      841 cgatgcgcgc cggctgcgcg ccgaaggcct ggcgctcgac gcccggttcg cgatcggcgg
      901 cgcgccgctc gagtcgcatc ggctgcatgt ctcgttgttc ggggtcggcg cctacctgcg
      961 cacgcgcccg gaggcggatg tcgagcgctg gcgtcgcgcc gcggcggcgg tgggctgcgc
     1021 agcgttcgag gtggtcttcg accgcatcgc cactttcggc ggcgcgggcc ggtcgctggt
     1081 gctgaaggcg gcggacgaat cggcgctggc cggattgcag gccctgtacg agtcgctggg
     1141 catcgcgctc gcgaacgccg gcgagcatgt cacgcagcgc cgcatcacgc cgcacatgag
     1201 cctgtcctac cacgggcggc cgatgcccga gaccgcgatc gagcccgtgc gctggcgcgc
     1261 gcacgagctg gtcttgatcg acagccacat cggcctgcat caccacgagg tgctgggacg
     1321 ctggcgcctg caggacgggt agccgcgcag gtggacaccg tccggcgatg agccggtcgc
     1381 ccgatgcgcg gcatcgcgtg cgccaacgcg gcgggagggc gagcgagctc tgatcccgcg
     1441 cgcgttcagc cgaggagcgg gccgcccggc gcttgcggaa tcgggcgggg cggctgcatc
     1501 cgcggcagga gttgctgcac caggagatcg acgagcaccc gcaccttgag cggcagcgcg
     1561 cgggcgtgcg gccagacggc gtgcagctcg tagccgtagg ggtgcggctc ctcgaagacc
     1621 tgcaccaggc gcccggcggc gaactccggc tcggccagcc agtgcggcac tcgcgcgagg
     1681 cccaggccgc cgcgcgcggc gtcggcgacg acctccaggc tgtcgaagac gaagcgggcg
     1741 cgcgggtcga actccgccgg cgcgccgcgc ccgtcgcgat gcagccaggg cttgggcgcg
     1801 ccctggcgcg cgtagccgat gaaggcgtgg gcgtcgcggc gcgcggccat ctcctcgaag
     1861 caggacggac ggccatggcg cgcgaagtag tcgggggacg cgacgacgca gacccactgg
     1921 tggcccagcg cgcgcgcggc gacgctgctg ctgtcggcga gcgggccgct gcggatcgcg
     1981 aggtcgatcc ggtcctccac gaggtcgacg cggcggtcgt cgaaggagac ctcgaacgac
     2041 agccccgcat gctcccgccc gagcgccagc agcagcggcg cgacgcagcg gcggccgatc
     2101 agctcgggca tcgtcaggcg cagccggccc gcgggctcgc ggcggccagc ctcgaagacg
     2161 gcgtcggcgg cctcgagctc cgccagcgcc cggcggcagc gctcgtagta ggcctggccg
     2221 tcgtcggtca ggctctggct gcgcgtcgtg cggcgaaaca agcgcgtgcc cagccgcgtc
     2281 tcgaggcggc ccacgctctt ggccaccgcg gagcgggtga gtcccatgcg cagcgccgcg
     2341 ccggcgaagg tgccggcttc caccgcggcg acgaagacgt cgataccgct caggcgttca
     2401 ctcattgatt cctctggatc gtcattgagg cgatgaaatt gaatcaccga ggactgatgg
     2461 gagtcaatac gatggccaca tcgatcactc accgaggaat gccgacatga ccgacaccct
     2521 gcgccaatgg cgattcgtgg acaacgggct cgcccacctg aagctgcacg agacggtgct
     2581 gcccgagccc gggccgggcc agatcctcgt gcgcatcgag gcggcgtcgc tgaactaccg
     2641 cgacctgatg ctggccgagg ggcgctacct gccgaccacg ctgccctacg tgccgggctc
     2701 ggacctggcc ggcgtcgtgc aggccgtggg cccgggcgtg cggcgctgga agggcggcga
     2761 acgcgtgatc ggcacctatg ccgccggctg gttggacggc gcggcgccgg cggacgtcgt
     2821 gccgctgggc ttcccgggcc ccggtgcgct ggcgagccac gtcgtcctcg acgagcagtg
     2881 ggccgtggcg gcgccgcagt cgctgacggc cgccgaggcg agcacgctgc cgatcgccgg
//
LOCUS       DASVLN010000704         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_306136, whole genome shotgun sequence.
ACCESSION   DASVLN010000704 DASVLN010000000
VERSION     DASVLN010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306136"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VF453_16660"
     CDS             <1..353
                     /locus_tag="VF453_16660"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7689346.1"
                     /translation="PDAPRGAAAAPLMPLDMHAGDGRHHRIHVEARIASNSQISLQHM
                     CEHGLGIARLAHADVLPALRRGALVRVLPQWRFAAMPVWAVTPRREVEEAKVRLAVEA
                     LRRHFADVPGLAAD"
     gene            complement(423..1577)
                     /locus_tag="VF453_16665"
     CDS             complement(423..1577)
                     /locus_tag="VF453_16665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX7689347.1"
                     /translation="MPVITCIEDLRRLARRRVPRMFYDYADSGSWTETTYRENESDFA
                     RIKLRQRVAVDMDNRSLATTLVGAHATMPVALAPTGLTGMQHADGEILAARAAEKFGV
                     PFTLSTMSICSIEDVAANTTRPFWFQLYVMRDRGFIERLIERARAARCSALVLTLDLQ
                     ILGQRHKDVRNGLSAPPKPTLANLVDLATKPAWCWGMLGTKRRSFGNIVGHVEGVGDV
                     SRLSEWTARQFDPNLNWGDVAWIKERWGGKLILKGILDAEDARLAAQTGADALIVSNH
                     GGRQLDGAMSSIAALPAIVDAVGDRLEVWMDGGIRSGQDVLKAWALGARGAMIGRAFL
                     YGLGAMGEAGVTRCLEILHKELDLTMAFCGHRDIQGVGRDILVPGTYPTA"
BASE COUNT          214 a          621 c          637 g          250 t
ORIGIN      
        1 cccccgacgc cccgcgcggc gccgccgccg cgccgctgat gccgctggac atgcacgccg
       61 gcgacggccg ccaccaccgc atccacgtcg aggcgcgcat cgcgagcaac agccagatct
      121 cgctgcagca catgtgcgag cacggcctgg gcatcgcgcg cctcgcgcat gccgacgtgc
      181 tgccggcgtt gcgccgcggt gcgctggtgc gcgtgctgcc gcagtggcgc ttcgcggcga
      241 tgccggtctg ggccgtcacg ccgcggcgcg aggtcgagga ggccaaggtg cggctcgcgg
      301 tcgaagcgtt gcggcgccat ttcgccgacg tgccgggcct cgcggcggac tgaggggcgg
      361 gtcgttcgtc gcgtcgccgg cgccgcgact ccccgcgatc gggatcggcc gcccgtatcc
      421 ggtcaggccg tcgggtaggt tcccggcacg aggatgtcgc gccccacccc ctggatgtca
      481 cggtgtccgc agaaggccat cgtgagatcc agctccttgt gcaggatctc gaggcagcgc
      541 gtcacgcccg cctcgcccat cgcgcccagg ccgtagagga aggcgcggcc gatcatcgcg
      601 ccgcgcgcgc ccagggccca cgccttgagc acgtcctggc cggagcggat gccgccgtcc
      661 atccagacct cgagccggtc gccgaccgcg tcgacgatcg ccggcagcgc cgcgatcgag
      721 ctcatcgcgc cgtccagctg ccgcccgccg tggttgctga cgatcagcgc gtcggccccg
      781 gtctgcgcgg cgagcctggc gtcctcggcg tcgaggatgc ccttgaggat cagcttgccg
      841 ccccagcgct ccttgatcca ggcgacgtcg ccccagttca ggttggggtc gaactgcctg
      901 gccgtccact cggacagccg gctgacgtcg cccacgccct cgacgtggcc gacgatgttg
      961 ccgaacgagc gccgcttcgt gcccagcatg ccccagcacc aggccggctt ggtcgcgagg
     1021 tcgaccaggt tcgcgagcgt cggctttggc ggtgccgaca ggccgttgcg cacgtccttg
     1081 tgccgctggc cgaggatctg caggtccagc gtcagcacga gcgcgctgca gcgcgcggcg
     1141 cgggcgcgct cgatcaggcg ctcgatgaag ccgcggtcgc gcatcacgta gagctggaac
     1201 cagaacggcc gcgtcgtgtt ggcggcgacg tcctcgatcg agcagatgct catcgtcgac
     1261 agcgtgaagg ggacgccgaa cttctcggca gcgcgcgcgg cgaggatctc gccgtccgcg
     1321 tgctgcatgc ccgtcaggcc ggtgggcgcg agcgccacgg gcatggtcgc gtgcgcgccg
     1381 acgagcgtcg tcgcgaggct gcggttgtcc atgtccaccg cgacgcgctg gcgcagcttg
     1441 atcctggcga agtcgctctc gttctcgcga taggtcgtct ccgtccacga gccggagtcg
     1501 gcgtagtcgt agaacatgcg cgggacgcgg cggcgcgcga gccggcgcag gtcttcgatg
     1561 caggtgataa cgggcatggc gaggcgactc ggtcggccgg ggagcccgca tggtagcgcc
     1621 gcgccgctcg cgggcggcgg tgccgcggcg tgaaccgccc gccgtggcgg gtgtcagaac
     1681 agcgtcggct cgtcggggcg atcgtccgcg gggcgcttgc gg
//
LOCUS       DASVLN010000705         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_521634, whole genome shotgun sequence.
ACCESSION   DASVLN010000705 DASVLN010000000
VERSION     DASVLN010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521634"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VF453_16670"
     CDS             <1..416
                     /locus_tag="VF453_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689348.1"
                     /translation="AALNTADLAQEANRPVDAGSVEAQVDQALALSMTRTPGDLARAQ
                     GLLDQVQRNASDQAAPWRRLARMLAYRLAEQKRAEDQAERSAQALRDSQRDAQRRYDQ
                     LNDKLEALKAIERNLNTRPGAGLPATAPAPSRPGP"
     gene            419..1792
                     /locus_tag="VF453_16675"
     CDS             419..1792
                     /locus_tag="VF453_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7689349.1"
                     /translation="MATAARILLVDDDPDLLRLLSMRLAAAGYRVTAVASAEEALVKI
                     ALERPQLVVSDVQLPGKDGLALFDAIRAQHPSLPVILLTAHGTIPDAVAATARGVYTY
                     LTKPFDGKALLDTIAKALAVEGTGEDGERGGDGEAWRAAIVSRSTRMAEVLAEARLVA
                     ASDASVLIRGESGTGKELVARAIHLASPRAGKPFVAVNCGAIPENLLESELFGHVKGA
                     FTGAVSNHEGLFQAAHEGTLFLDEIGDMPVALQVKLLRVLQERLVRPVGASHAVPVDV
                     RILSATHRDLDAAIAEGSFRSDLYYRLAVVSLTLPTLAERREDIPLLAHHFLSRLAGK
                     YGRKVNGFAPDALKALTTASWPGNVRQLHNVVEQVTALATTPLVPLALVQRALRVPSM
                     DVLSYTEARARFERDYLVNLLKIADGNVADAARLADRNRTEFYRLMQKHGLTPGLFRS
                     PDGPVAD"
BASE COUNT          306 a          797 c          726 g          276 t
ORIGIN      
        1 tggccgcgct caacaccgcc gacctcgcgc aggaggccaa ccgcccggtc gacgccggtt
       61 cggtggaggc gcaggtcgac caggcgctcg cgctgagcat gacccgcacc cccggcgacc
      121 tcgcgcgggc gcagggcctg ctcgaccagg tgcagcgcaa cgcgtccgac caggccgcgc
      181 cgtggcgccg cctcgcgcgc atgctggcct accggctcgc cgagcagaag cgcgccgagg
      241 atcaggccga gcgcagcgcc caggccctgc gcgacagcca gcgcgacgcg cagcgccgct
      301 acgaccagct caacgacaag ctcgaggcgc tgaaggcgat cgagcgcaac ctcaacacgc
      361 gcccgggcgc gggcctgccc gccaccgcgc ccgcgccatc gcgtccgggg ccctgagcat
      421 ggcgaccgcc gcgcgcatcc tgctggtcga cgacgacccc gacctgctgc gactgctgtc
      481 gatgcggctc gcggccgccg gctaccgcgt cacggcggtc gcgtcggccg aggaggcgct
      541 cgtcaagatc gcgctcgagc gcccgcaact ggtcgtgagc gacgtgcagc tgccgggcaa
      601 ggacggcctc gccctgttcg acgcgatccg cgcgcagcat ccgtcgctgc cggtgatcct
      661 gctgacggcg cacggcacca tccccgacgc cgtggccgcc acggcgcgcg gcgtctacac
      721 ctacctgacc aagcccttcg acggcaaggc gctgctggac acgatcgcca aggcgctggc
      781 ggtcgagggc acgggcgagg acggcgagcg cggcggagac ggcgaggcct ggcgtgcggc
      841 gatcgtgagc cgttccacgc gcatggccga ggtgctggcg gaggcgcggc tggtcgcggc
      901 gtccgacgcg agcgtgctga tccgcggcga gagcggcacc ggcaaggagc tcgtcgcgcg
      961 cgcgatccac ctcgcgagcc cgcgcgccgg caagcccttc gtcgccgtga actgcggcgc
     1021 gatcccggag aacctgctcg agtcggagct cttcgggcac gtcaagggcg cgttcacggg
     1081 ggcggtgtcg aaccacgagg gcctgttcca ggccgcgcac gagggcacgc tgttcctcga
     1141 cgagatcggc gacatgcccg tcgcgctgca ggtcaagctg ttgcgcgtgc tgcaggagcg
     1201 cctcgtgcgg cccgtgggcg cgagccacgc cgtgccggtg gacgtgcgca tcctgtcggc
     1261 cacgcaccgc gacctcgacg ccgccatcgc cgagggcagc ttccgcagcg acctctacta
     1321 ccggctcgcg gtcgtctcgc tgacgctgcc gacgctggcc gagcggcgcg aggacatccc
     1381 gctgctcgcc caccacttcc tgtcgcggct ggcgggcaag tacggccgca aggtcaacgg
     1441 cttcgcgccc gacgcgctca aggcgctgac gaccgcctcg tggccgggca acgtgcgcca
     1501 gctgcacaac gtcgtcgagc aggtgacggc gctcgcgacg acgccgctcg tgccgctcgc
     1561 gctcgtgcag cgcgcgctgc gcgtgccgag catggacgtg ctgagctaca ccgaggcacg
     1621 cgcgcgcttc gagcgcgact atctcgtcaa cctgctcaag atcgccgacg gcaacgtcgc
     1681 cgatgccgcg cgcctggccg accgcaaccg gacggagttc taccgcctga tgcagaagca
     1741 cggcctgacg ccggggctgt ttcgctcccc ggacggccct gtcgccgatt agcgacaagg
     1801 gaaaatcctt gtatatcaag gacttggccg gatagtcgcg cgcgctgtcg ccgccgcccg
     1861 acgctttcgg gcgattttcg gccgtccagc gacagcctgc cgcaagccgg ctctgcaata
     1921 cgacttcaag ccattgattc cacgcgcttt ttgagactgg catgaacgtt gcggaagggg
     1981 acgtcaatcc cccttccgaa cgattcacac acatgcccgt cgcgaccggc ctcccgcctc
     2041 ggtccccctt cgaaacgccc cggcgcgccg cttcgtgcgc gccgcgcgcg gacgcctgcg
     2101 cgccc
//
LOCUS       DASVLN010000706         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_411681, whole genome shotgun sequence.
ACCESSION   DASVLN010000706 DASVLN010000000
VERSION     DASVLN010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411681"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            415..1056
                     /locus_tag="VF453_16680"
     CDS             415..1056
                     /locus_tag="VF453_16680"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7689350.1"
                     /translation="MQPPPPITRALLIACTVLLFVCHIPALQNALILWLTLTPVQGGF
                     FFGYQAVTYALVHLDPFQWFFNMLGLYFFGAELENLWGERRFVQFLLASVLMGALFYI
                     VLTLVFGASVPLLGSTGALFGMLVAFGMLFPNRPLSLYFVMQVRMRTAVLVFIGIEVF
                     LMLGNLGGLSGAWIGNLSHLGGALGGYLMILYWRWRPPSFRRTTKKPPLRRVH"
     gene            complement(1090..>1538)
                     /locus_tag="VF453_16685"
     CDS             complement(1090..>1538)
                     /locus_tag="VF453_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7689351.1"
                     /translation="TGARASVLRIPGIYAPDREGGRPQDRVRRGTPSLVPEDDGYTNH
                     VHAEDLARACLAALWRGLPQRVVHVVDDSDMKTGDYYALVADLTGLPRPARVTRAQAR
                     AQGLYSEAQWSFLEESRRLANDRLKRELRVRLRYPTVADALKPGGQ"
BASE COUNT          194 a          540 c          565 g          239 t
ORIGIN      
        1 gtccgcgata gcccgccgca gtggccccgg cgaggtggga gcctgggacg aggccagcga
       61 gcccgacacg gacttcgcgc cggccgccgt gcggctgacg gagagatcgg aaaagaggga
      121 cgagagggac ggcatggaca gggcgccggc gggacatggg gccctgcgga ggccctcctc
      181 cgccggcccg cgctgccgac ccgtggccat ttcacgtcaa cggcccggct gcggcggcca
      241 tcaggcgaag atgctgacgc gcaagcgaag ggcgtggcct cgtcgatccc aggcgcacag
      301 gcagcccggc cgggcccgga ggccgggtat ccggacggtc gatcccgcgc cgcgccgcga
      361 cggccgcgag cgaggctcgt ggcacactgc gcctaagctc ccgagacgcc gtccatgcaa
      421 ccgccgccgc ccatcacccg cgccctgctc atcgcctgca ccgtgctgct gttcgtctgc
      481 cacatcccgg cactgcagaa cgcgctgatc ctgtggctga cgctgacgcc cgtgcagggc
      541 ggcttcttct tcggctacca ggccgtcacc tacgcactgg tgcacctgga cccgttccag
      601 tggttcttca acatgctcgg cctgtacttc ttcggcgccg agctggagaa cctgtggggc
      661 gagcgccgct tcgtgcagtt cctgctggcc agtgtcctga tgggcgcgct gttctacatc
      721 gtgctgacgc tggtgttcgg ggcctcggtg ccgctgctcg gctcgaccgg ggcgctgttc
      781 ggcatgctgg tcgcgttcgg catgctgttc cccaaccggc cgctgtcgct gtacttcgtc
      841 atgcaggtgc gcatgcgcac cgcggtgctg gtgttcatcg gcatcgaggt cttcctgatg
      901 ctgggcaacc tgggcgggct gtcgggcgcg tggatcggca acctctcgca cctgggcggc
      961 gcgctgggcg gctacctgat gatcctgtac tggcgctggc ggccgccctc gttccggcgg
     1021 acgaccaaga agccgccgct gcggcgggtg cactgaaggc cggcgacggg gcgcccgcgg
     1081 gcgcccttct cattggccgc cgggtttcag cgcatcggcc acggtcggat agcgcagccg
     1141 cacgcgcagc tcgcgcttga ggcgatcgtt ggcgaggcgg cgcgactcct cgaggaagct
     1201 ccactgggcc tcgctgtaca agccctgcgc gcgagcctgc gcacgcgtga cgcgcgcggg
     1261 gcgcggcagg ccggtgaggt cggcgacgag ggcgtagtag tcgcccgtct tcatgtcgga
     1321 gtcgtcgacc acgtgcacga cgcgctgcgg caggccgcgc cacaacgccg cgaggcaggc
     1381 gcgtgcgagg tcctcggcgt ggacgtgatt ggtgtagccg tcgtcctcgg gcaccagcga
     1441 cggcgtgccg cggcgcacgc ggtcctgcgg gcggccaccc tcgcggtcgg gcgcgtagat
     1501 gcccgggatg cgcagcacgc tcgcgcgcgc accggtcg
//
LOCUS       DASVLN010000707         4059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_468362, whole genome shotgun sequence.
ACCESSION   DASVLN010000707 DASVLN010000000
VERSION     DASVLN010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468362"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /gene="lptA"
                     /locus_tag="VF453_16690"
     CDS             <1..621
                     /gene="lptA"
                     /locus_tag="VF453_16690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEX7689352.1"
                     /translation="RRPGAAHALGALVALAAIVASAPAHAERADRTKPMTIDSSGSQP
                     DVVDLGKHSAVFTGNVVITQGTLVIHADRVEVSEDADGQRLGTAFGTPGHPATFRQKR
                     DGGDDEYSEGEADKIRYDSAANRVYFDGNAHLRMLRGTTVTDQASASLITYDTAAEAF
                     SLTGKSAANPGGRTTVVFTPKPASSAPAPKPLELPSTTPAASGPAR"
     gene            618..1472
                     /gene="lptB"
                     /locus_tag="VF453_16695"
     CDS             618..1472
                     /gene="lptB"
                     /locus_tag="VF453_16695"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX7689353.1"
                     /translation="MSDASGSPTPVRAGSTVSPSATPAAAATTPRPVVAVAAASRLEA
                     TGLKKSYGARLVVKEVHLAVNAGEVVGLLGPNGAGKTTTFYMVVGLVRADAGDIRIDG
                     RPISAKPIHQRSRLGLSYLPQEASIFRKLTVDENIRAVLELQYDANGKALKPAEIDKR
                     LDGLLADLSLTHLRESPAPALSGGERRRVEIARALATQPRFILLDEPFAGVDPIAVIE
                     IQRIIGFLKARGIGVLITDHNVRETLGICDRAYIISEGRVLAEGTPAEIVENADVRRV
                     YLGEHFRM"
     gene            1489..3036
                     /locus_tag="VF453_16700"
     CDS             1489..3036
                     /locus_tag="VF453_16700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX7689354.1"
                     /translation="MKASLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEIEQMMEQN
                     PFLEPEEDAHGPDTAFDLAAPPAPAADARTSEPTAEVGSEGGDEPAGVDAADFGTTER
                     DDWENGTERDDFDGIREMPGTASGAGADGDDEFERNNRAGASVSLEDHLRAQLVSLRL
                     SPEDAAAVHVLIESLNGDGYLADPLEEIVERLAEVLGIADDDAEGREELLGQLQCGLN
                     FLQSMEPTGVGARTLGECLRLQLRQVPVTAANAGAVKAALAMCDEHLALLARRDFRKL
                     AQLTGTDEATVRMAQALIVACEPKPGRPFARAEEQIIVPDVIVTRVARQWKAVLNPDV
                     MPKLRVNDLYAQAIKSQRASGSALTARLQEARWFIRNIQQRFDTILRVSQAIIERQKN
                     FFSHGAIAMKPLVLREIADELGLHESTISRVTTAKYMATPFGTYELKYFFGSSLNTDA
                     GGNASSTAVRALIKQFVAAESPAKPLSDSQLAELLEGQGIQVARRTVAKYREALKIAP
                     ANLRKAV"
     gene            3101..>4059
                     /locus_tag="VF453_16705"
     CDS             3101..>4059
                     /locus_tag="VF453_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX7689355.1"
                     /translation="MSKDFLFVSCANGSEEWLAAECLRICGPDQGVEAGRGGVMVDAS
                     PENAMRLNLWSRIAQRVLWPLADAEYRDERDLYDLACRVRWQEWITPDETLRVDVTAQ
                     RSPLRSLNFAALKVKDAICDVMRDRFDARPSVDTRWPDLQVSLHVDATHAWLAIDLSG
                     EALFKRGWREQQGEAPLKETLAATMLAAAGWTGAAEGGGLLDPCCGAGTIAIEAAQLA
                     CNVAPGLTRRFAFERLQPFRDHAQRWGRMQVEAREGVAKPAAPIWAGDVSFRMTDFAA
                     RNAERAGVAHAIEFKTADALQRPAPADRGTLMLNPPYGERIDA"
BASE COUNT          636 a         1519 c         1423 g          481 t
ORIGIN      
        1 cgccgtccgg gcgcggcgca cgcgctgggc gccctggtcg cgctggccgc gatcgtcgcc
       61 tctgcgccgg cgcacgccga gcgcgccgac cgcaccaagc cgatgacgat cgacagctcc
      121 ggcagccagc ccgacgtggt cgacctgggc aagcacagcg cggtcttcac cggcaacgtc
      181 gtgatcaccc aggggacgct cgtgatccac gccgaccgcg tcgaggtgag cgaagacgcc
      241 gacggccagc gcctgggcac ggccttcggc acgccgggcc atcccgccac cttccgccag
      301 aagcgcgacg gcggcgacga cgagtacagc gagggcgagg ccgacaagat ccgctacgac
      361 agcgccgcca accgcgtcta cttcgacggc aacgcgcacc tgcgcatgct gcgcgggacg
      421 acggtcaccg accaggcgag cgcctcgctc atcacctacg acacggccgc cgaggcgttc
      481 tcgctgacgg gcaagtctgc cgcgaacccg ggcggccgca cgaccgtggt cttcacgccc
      541 aagccggcga gttccgcgcc ggcgcccaag ccgctggaac tgccatcgac cactcccgcc
      601 gcttccggcc ccgcacgatg agcgacgcga gcggatcccc cacgccggtg cgggccggca
      661 gcacggtctc gccgtccgcg acgcccgcag ccgccgccac gacgccgcgc ccggtcgtgg
      721 ccgtggctgc cgcgagccgc ctcgaggcga cggggctgaa gaagtcctac ggcgcacgcc
      781 tggtcgtcaa ggaagtgcac ctggcggtca acgcgggcga ggtcgtcggc ctgctgggcc
      841 ccaacggcgc cggcaagacg acgacgttct acatggtcgt cggcctcgtg cgcgccgacg
      901 ccggcgacat ccgcatcgac ggccggccca tctcggccaa gccgatccac cagcgctcgc
      961 gcctgggcct gagctacctg ccgcaggagg cctcgatctt ccgcaagctg acggtcgacg
     1021 agaacatccg cgcggtgctg gagctgcagt acgacgccaa cggcaaggcg ctgaagcctg
     1081 ccgagatcga caagcgcctg gacggcctgc tcgccgacct gagcctcacg cacctgcgcg
     1141 agtccccggc gccggcgctg tccggcggcg agcgccggcg cgtcgagatc gcgcgcgcgc
     1201 tcgccacgca gccgcgcttc atcctgctgg acgagccgtt cgcgggcgtc gacccgatcg
     1261 cggtcatcga gatccagcgc atcatcggct tcctcaaggc gcgcgggatc ggcgtgctga
     1321 tcaccgacca caacgtgcgc gagacgctgg gcatctgcga ccgcgcgtac atcatcagcg
     1381 aaggccgggt gctggccgag ggcacgcccg cggagatcgt cgagaacgcc gacgtccgcc
     1441 gcgtgtacct cggcgaacac ttccgcatgt gacgacgagg gccggacgat gaaagcgtcg
     1501 ctgcaggtcc gcctgtcgca acacctcgcg ctgacgcccc aactgcagca gtcgatccgg
     1561 ctgctgcagc tctcgaccct ggagctgcac caggagatcg agcagatgat ggagcagaac
     1621 cccttcctcg agcccgagga ggacgcgcac gggccggaca ccgcgttcga cctcgccgcg
     1681 ccgccggcgc cggcggcgga cgcgcgcacc tccgaaccga cggccgaagt cggcagcgag
     1741 ggcggcgacg agccggccgg cgtggacgcc gccgacttcg gcaccaccga gcgcgacgac
     1801 tgggagaacg gcaccgagcg cgacgacttc gacggcatcc gcgagatgcc cggcaccgcg
     1861 tcgggcgcgg gcgcggacgg cgacgacgag ttcgagcgca acaaccgcgc cggcgcgagc
     1921 gtctcgctgg aggaccacct gcgcgcgcag ctggtgagcc tgcgcctgtc gcccgaggac
     1981 gcggccgcgg tgcacgtgct gatcgagtcg ctcaacggcg acggctacct cgccgatccg
     2041 ctcgaggaga tcgtcgagcg cctggccgag gtgctgggca tcgccgacga cgacgcggag
     2101 ggccgcgagg aactgctcgg ccagctgcag tgcggcctga acttcctcca gagcatggag
     2161 cccaccggcg tgggcgcgcg cacgctcggc gaatgcctgc ggctgcagct gcgccaggtc
     2221 ccggtgacgg cggccaacgc gggcgcggtc aaggcggcgc tcgcgatgtg cgacgagcac
     2281 ctcgcgctgc tggcgcggcg cgacttcagg aagctcgccc agctcaccgg cacggacgag
     2341 gccaccgtcc gcatggcgca ggcgctgatc gtcgcctgcg aacccaagcc cggccggccg
     2401 ttcgcgcgcg ccgaggagca gatcatcgtg cccgacgtca tcgtcacgcg tgtcgcgcga
     2461 cagtggaagg cggtgctcaa tcccgacgtc atgcccaagc tgcgcgtcaa cgacctctac
     2521 gcgcaggcga tcaagtcgca gcgcgccagc ggcagcgcgc tgacggcgcg gctgcaggag
     2581 gcgcgctggt tcatccgcaa catccagcag cgcttcgaca ccatcctgcg cgtctcgcag
     2641 gccatcatcg agcgccagaa gaacttcttc agccacggcg cgatcgcgat gaagccgctg
     2701 gtgctgcgcg agatcgccga cgagctgggg ctgcacgagt ccacgatctc ccgcgtgacc
     2761 accgccaagt acatggccac gccgttcggc acctacgagc tgaagtactt cttcggctcg
     2821 tcgctcaaca cggatgccgg cggcaacgcg tcgtccacgg cggtgcgcgc gctgatcaag
     2881 cagttcgtcg cggccgagag cccggccaag ccgctgtccg acagccagct cgccgagctg
     2941 ctggagggcc agggcatcca ggtcgcgcgc cgcacggtcg ccaagtaccg cgaggcgctc
     3001 aagatcgcgc ccgcgaacct gcgcaaggcc gtctgagcct cccccaacgg cgtccgcgac
     3061 gggcgggcgc cacccacgtg ccgaagggtg caccgccatc atgagcaagg acttcctctt
     3121 cgtttcctgc gccaacggca gcgaggaatg gctcgccgcc gaatgcctgc ggatctgcgg
     3181 ccccgaccag ggcgtcgagg cgggccgcgg cggcgtgatg gtggacgcga gccccgagaa
     3241 cgcgatgcgc ctgaacctgt ggagccgcat cgcgcagcgc gtgctgtggc cgctggccga
     3301 cgccgagtac cgcgacgagc gcgacctcta cgacctcgcc tgccgcgtgc gctggcagga
     3361 gtggatcacg cccgacgaga cgctgcgcgt ggacgtgacg gcgcagcgct cgccgctgcg
     3421 cagcctcaac ttcgcggcgc tgaaggtcaa ggacgcgatc tgcgacgtga tgcgcgaccg
     3481 gttcgacgcg cgcccgagcg tcgacacgcg ctggcccgac ctgcaggtga gcctgcacgt
     3541 cgacgcgacg cacgcgtggc tggcgatcga cctgagcggc gaggccctgt tcaagcgcgg
     3601 ctggcgcgag cagcagggcg aggcgccgct caaggagacg ctcgccgcga cgatgctcgc
     3661 ggccgcgggc tggaccgggg cggccgaggg cggcggcctg ctcgacccgt gctgcggcgc
     3721 cgggacgatc gcgatcgagg ccgcgcagct cgcctgcaac gtcgcgcccg gcctcacgcg
     3781 ccgcttcgcg ttcgagcgcc tgcagccctt ccgcgaccat gcgcagcgct ggggccggat
     3841 gcaggtcgag gcgcgcgagg gcgtcgcgaa gcccgccgcg ccgatctggg ccggcgacgt
     3901 gtcgttccgg atgaccgact tcgccgcccg caacgccgag cgcgcgggcg tcgcccacgc
     3961 catcgagttc aagaccgccg acgccctgca gcgcccggcg ccggccgatc gcggcacgct
     4021 gatgctcaac ccgccctacg gcgagcgcat cgacgccaa
//
LOCUS       DASVLN010000708         5621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_251511, whole genome shotgun sequence.
ACCESSION   DASVLN010000708 DASVLN010000000
VERSION     DASVLN010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251511"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VF453_16710"
     CDS             <1..473
                     /locus_tag="VF453_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7689356.1"
                     /translation="HALEALAMVAERSAHERSWSAPARMRLREARCCYGHLAGRRGVA
                     LLQRMLGRGWLSLAPDGYALTPAGAGGLAELGLDATAWLEATPRKRAAYPCLDWSERR
                     DHLAGPLAASVLSHFVARGWLRREPGERALTLTPPGRLALAALVELEDETPIAG"
     gene            696..1157
                     /locus_tag="VF453_16715"
     CDS             696..1157
                     /locus_tag="VF453_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7689357.1"
                     /translation="MSVDARLRALGIELPPIAIPAAAYLPFVQTGSLVFLSGHIAKKD
                     GKPWVGQLGLTMSTEEGKAAARAIAIDLMGTLKAAAGGDLDRVTRIVKVMSLVNSTPA
                     FTEQHLVTNGCSELLGDVFGDAGKHARSAFGVAQIPMGACVEIELVAELAA"
     gene            1154..1645
                     /locus_tag="VF453_16720"
     CDS             1154..1645
                     /locus_tag="VF453_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX7689358.1"
                     /translation="MRIRLRIPQVLAIVAVLSFGALAAALVAQYVFDMQPCPWCVLQR
                     LIFVVIGVLALVGAATRGAVRRAATGLAIAAALSGIASALWQQFYAASENSCDLSLAE
                     RITTALHLDRLLPQVFIAYASCGDAAVSILGIPFAIWSCAMFVLVAVLLAWTLRAALR
                     TGR"
     gene            1722..2948
                     /locus_tag="VF453_16725"
     CDS             1722..2948
                     /locus_tag="VF453_16725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7689359.1"
                     /translation="MRARLDAEVCVVGAGFAGLATAVGLIERGVRDVVVLESEAVGHG
                     ASGRNGGFVFGGFSLDCARLLEQQGPERARAMYALTRRAVQTIRDRIARHAIACDAVE
                     GGVVLANWFDDPAPLLRRQQLMARHFDTQWEYLTHERLGDYTRSARYADGLLEPDAFH
                     FHPLKYARGLAEAIERQGGRIFERSAVTAIAARAGGHEVRVHGTDGMQGVVHARHVVV
                     AGGGYLRGLVPALERAMLPIATYVVATEPLGEALARSIPGKAAIYDTRFSFDYYRPLP
                     DTRLLWGGRISILDRGPAPIARLLKADLCKVYPELRDARIEHAWGGLMSYARHEMPQI
                     GRLPDGCWHALGFGGHGVGPTTAAGELLAAAIADGEPIPAGFADYGLPRVWGPAGMLA
                     AQMRYNWAEFRDWLAQ"
     gene            complement(3006..3887)
                     /locus_tag="VF453_16730"
     CDS             complement(3006..3887)
                     /locus_tag="VF453_16730"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX7689360.1"
                     /translation="MRQDIDFSGVWIPIVTPFRDGQVDELALAALTRRLDGQGIAGFI
                     VCATTGEAPLLDDAERDRVLATVRAHATRPVVLGTSGSTAAQVLRKMDAAARHAPAAF
                     LVTPPPYLKPSQDALRVFFTQVADAAPAPVMLYDIPSRTGVRLELETVLALAAHPRVV
                     AIKDCGGRAEQTEALVIDGRLKVLCGNDAEWFASRCLGGDGAVAASAHVRTDLFVAFD
                     RAMARGDLVRGRELWRELRPLTVGFFDEPSPAPVKAVLAAAGECSDEVRAPLTPASAG
                     LRARLADLVARLPGSAA"
     gene            complement(4166..5191)
                     /locus_tag="VF453_16735"
     CDS             complement(4166..5191)
                     /locus_tag="VF453_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7689361.1"
                     /translation="MDYRRLGASGFKVPELSLGTGTFGGGGEFFAAWGSTQAEGARRL
                     LDICLDAGLNMFDSADVYSNGRAEEVLGEAIAGRRDRVIVSTKATFRRGSGPNDVGSS
                     RHHLRDAVEGSLRRLRTDYIDLFQLHGFDALTPVEETLQTLDDLVREGKIRYLGVSNF
                     SGWHLMKSLAASERLGLQRYVAHQAYYSLVGRDYEWELMPLGLAEGVSAVVWSPLGWG
                     RLTGRIRRGSGLPAQSRLHKTADAGPPVPDELLFRVVDALEAVAEETGRSVPQVALNW
                     LLQRPTVASVIIGARDEAQLRDNLGAVGWALSTQQMRRLDEASAVTKAYPYWHQAQFG
                     ERNPSPV"
     gene            5437..>5621
                     /locus_tag="VF453_16740"
     CDS             5437..>5621
                     /locus_tag="VF453_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689362.1"
                     /translation="MPNRRLSATLVLLASALLEVALRLRKRPCMHGRGRPLALGWLVA
                     LATVAGAATSRPPDAPP"
BASE COUNT          756 a         2100 c         1998 g          767 t
ORIGIN      
        1 cgcacgcgct cgaagcgctc gcgatggtgg ccgagcgatc ggcgcacgag cgcagctggt
       61 ccgcgcccgc gcgcatgcgg ctgcgcgaag cgcgctgctg ctacggccat ctggccgggc
      121 gacgcggcgt ggcgctgttg cagcgcatgc tgggccgcgg atggctgtcg ctcgcccccg
      181 acggctacgc gctgacgccg gcgggcgccg gcggactggc ggaattgggg ctggatgcca
      241 ccgcctggct ggaggccacg ccccgcaagc gcgcggccta tccctgcctc gactggtccg
      301 agcgccgcga ccacctcgcc ggcccactgg ccgcgagcgt gctgtcgcac ttcgtcgcgc
      361 gcggttggct gcggcgcgag cccggcgagc gagcgctgac gctcacgcca ccgggcaggc
      421 tggcactggc ggcgctggtc gagctcgagg acgagacccc aatcgccggt tgaaggcctc
      481 gtcacgcgcc gcgacccggc gcatgcaccc tggcccctgc gccagcacct gcggcggccc
      541 gcggcctgca ccccgtgccg cccggcgtgc cggacaccgc cgtccattcg ccgagcgacc
      601 acgggtcttt gccggcgcgt gtagcatcat cgccggctcg accgccgccc tcgtcccgac
      661 ggcccggcgc agccacatcc acggagaact gaaccatgtc ggtcgacgcc cgcctgcgcg
      721 ccctaggaat cgaactgccg ccgatcgcca tcccggccgc ggcctacctg cccttcgtcc
      781 agaccggctc gctggtcttc ctctccggcc acattgccaa gaaggacggc aagccctggg
      841 tcggccagct cggcctgacg atgtcgaccg aggaaggcaa ggccgccgcg cgcgcgatcg
      901 cgatcgacct gatgggcacg ctcaaggccg cggccggcgg cgacctggac cgcgtgacgc
      961 gcatcgtcaa ggtgatgagc ctggtcaaca gcacgccggc gttcaccgag cagcacctgg
     1021 tcaccaacgg ttgctcggag ctgctcggcg acgtcttcgg cgatgccggc aagcacgcgc
     1081 gcagcgcctt cggcgtcgcg cagatcccga tgggcgcgtg cgtcgagatc gaactggtcg
     1141 ccgagctcgc ggcatgagga tccgcctgcg catcccccag gtgctcgcca tcgtcgcggt
     1201 gctgtcgttc ggcgcgctcg cggccgcact cgtcgcgcag tacgtgttcg acatgcagcc
     1261 gtgcccctgg tgcgtgctgc aacgcctgat cttcgtggtg atcggcgtgc tcgcgctggt
     1321 cggtgcggcg acgcgcggcg cggtgcggcg cgccgcgacc gggctcgcga tcgcggccgc
     1381 gctctccggc atcgcgtcgg cgctctggca gcagttctac gccgccagcg agaactcgtg
     1441 cgacctgagc ctcgccgagc gcatcacgac cgcactgcac ctcgaccgcc tgctgccgca
     1501 ggtattcatc gcctacgcct cctgcgggga cgcggcggtg tccatcctgg gcatcccgtt
     1561 cgcgatctgg agctgcgcga tgttcgtgct ggtggcggtg ctgctcgcgt ggacgctgcg
     1621 tgcggcgctg cgcaccgggc gctgaggcgc gcggccggca cacggtgaag ccgggtacct
     1681 actacgcggc cagcgcgggc ccggcgacga cccgggcacc gctgcgcgca cgcctggatg
     1741 ccgaggtctg cgtggtcggc gccggcttcg cggggctcgc gacggcggtc ggcctcatcg
     1801 agcgcggcgt gcgcgacgtc gtcgtgctgg agtcggaagc cgtcggccac ggcgcctccg
     1861 gccgcaacgg cggattcgtg ttcgggggct tcagcctcga ctgcgcgcgc ctgctggagc
     1921 agcagggccc cgagcgtgcc cgcgcgatgt acgcattgac ccgccgcgcg gtgcagacca
     1981 tccgcgatcg catcgcccgt cacgcgatcg cctgcgacgc ggtcgagggt ggcgtcgtcc
     2041 tcgccaactg gttcgacgac ccggcgccgc tgctgcgtcg ccagcagctg atggcgcgcc
     2101 acttcgatac ccagtgggag tacctgacgc acgagcgtct cggggactac acccgctcgg
     2161 cgcgctacgc cgacggcctg ctcgagcccg acgccttcca cttccatccc ctgaagtacg
     2221 cccgcggcct cgccgaggcg atcgagcgcc agggaggccg gatcttcgag cgcagcgccg
     2281 tcaccgcgat cgcggcgcgc gcgggcggcc acgaggtgcg cgtccacggc accgacggca
     2341 tgcagggcgt cgtgcatgcg cgacacgtcg tcgtcgccgg cggcggctac ctgcgcggcc
     2401 tcgtgccggc gctggagcgt gcgatgctgc ccatcgcgac ctacgtcgtc gcgaccgagc
     2461 cgctgggcga ggcgctcgcg cgctcgattc ccggcaaggc ggcgatctac gacacgcggt
     2521 tctcgttcga ctactaccgt cccctgcccg acacgcgact gctgtggggc ggccgcatct
     2581 ccatcctcga ccgcggcccc gcgcccatcg cgcgcctgct caaggccgac ctgtgcaagg
     2641 tctatcccga gctgcgcgac gcccgcatcg agcacgcgtg gggcgggctc atgagctacg
     2701 cgcgccacga gatgccgcag atcggccgcc tgcccgacgg ttgctggcac gcgctggggt
     2761 tcggtggcca cggcgtcggg ccgacgacgg ccgccggcga acttctcgcc gccgccatcg
     2821 ccgacggcga gcctatcccc gccggattcg cggactacgg gctgccacgc gtctggggcc
     2881 cggccggcat gctggcggcg cagatgcgct acaactgggc ggagttccgc gactggctgg
     2941 cgcagtgagg gtgcgctgcc gcgatcgccg gccgcacccc aggacgcacc cgaggccgca
     3001 ctcgctcagg ccgccgatcc gggcagccgc gcgaccaggt cggcgagccg cgcgcgcaag
     3061 cctgcggatg ccggggtcaa gggcgcacgg acttcgtcgc tgcattcgcc tgccgcagca
     3121 agcacggcct tgacgggcgc ggggctgggc tcgtcgaaga agccgaccgt cagcggccgc
     3181 agttcgcgcc agagctcccg gccacgcacg aggtcgccgc gcgccatcgc ccggtcgaag
     3241 gccacgaaca ggtcggtgcg cacgtgcgcg ctggcggcga cggcgccgtc gccgccgagg
     3301 cagcgcgagg cgaaccactc ggcgtcgttg ccgcacagca ccttcaggcg accgtcgatg
     3361 acgagcgcct ccgtctgctc cgcgcggccg ccgcagtcct tgatcgcgac cacgcgcgga
     3421 tgcgccgcga gcgcgagcac ggtctccagc tccagccgca cgccggtgcg cgacgggatg
     3481 tcgtagagca tcaccggcgc gggtgcggcg tcggcgacct gcgtgaagaa gaccctcagc
     3541 gcgtcctgcg acggcttgag gtagggcggc ggtgtgacga gaaaggccgc aggcgcgtgg
     3601 cgagccgcgg catccatctt gcgcagcacc tgcgccgccg tgctgcccga ggtgccgagc
     3661 acgaccggac gggtcgcatg ggccctcacc gtggcaagca cccggtcgcg ctcggcgtcg
     3721 tccagcagcg gcgcctcgcc ggtcgtcgcg cagacgatga agccggcaat gccctgccca
     3781 tccagccgac gggtcagcgc cgcgagtgcc agctcgtcga cctggccgtc gcgaaagggt
     3841 gtgacgatgg ggatccagac cccggaaaaa tcgatgtctt ggcgcacgcg aagagtcctc
     3901 tacaggcaac gaccgatcga agacgtcgtc cgagaagcgc gggacaccgg agccggcatg
     3961 cgtggagggc cgaaggcctg gttccggcgg gatccgtcgc tcatccgacg acggaacccg
     4021 aagctccggt cagacgagcg acggcttttt ggctttgacg gcgacccggc caggcccctc
     4081 cgcgcgcatg ggcgcgcggg ggaaagcgag gacgtggcaa gccggcatgg gcgcgatgtt
     4141 aacccgcccg cgacctgcgc atccatcaga cgggtgacgg gttgcgctcg ccgaactgcg
     4201 cctggtgcca gtacgggtac gccttcgtca cggcgctcgc ctcgtcgagg cggcgcatct
     4261 gctgggtcga cagggcccag ccgacggcgc cgaggttgtc gcgcagttgc gcctcgtcgc
     4321 gcgcgccgat gatcacggag gccaccgtcg gccgctgcag gagccagttc agggccacct
     4381 gcggcacgct gcggccggtc tcctcggcca cggcctccag cgcgtcgacg acccggaaca
     4441 gcaactcgtc gggcacgggc ggtcccgcgt cggccgtctt gtgcagccgg ctctgcgccg
     4501 gcaggccgct gccgcgccgg atccggccgg tgagcctgcc ccagcccagg ggactccaca
     4561 cgaccgccga cacgccttcg gccagcccga gcggcatcag ctcccactcg tagtcgcggc
     4621 ccaccagcga gtagtacgcc tggtgcgcca cgtagcgctg cagtcccagg cgctcggacg
     4681 ccgcgagcga cttcatcagg tgccagcccg agaagttgga gacgccgagg tagcggatct
     4741 tgccctcgcg caccaggtcg tcgagcgtct gcagggtctc ctcgacgggc gtcagcgcgt
     4801 cgaagccgtg cagctggaac aggtcgatgt agtcggtgcg cagccggcgc agcgaaccct
     4861 cgaccgcatc gcgcaggtgg tggcgcgagg atccgacgtc gttgggcccg ctgccgcggc
     4921 ggaacgtcgc cttggtcgac acgatcacgc ggtcgcgccg gccggcgatc gcctcgccca
     4981 gcacctcctc cgcacgcccg ttcgaataga cgtccgcgct gtcgaacatg ttgagcccgg
     5041 cgtccaggca gatgtcgagc agccggcgcg caccctcagc ctgggtcgag ccccaggcgg
     5101 cgaagaactc gcccccgccg ccgaaggtgc cggtgcccag gctcagttcg ggcaccttga
     5161 agcccgaggc tcccagtcgt cggtagtcca tgcgtcgctc ctgaatcgct cgtgtcgaag
     5221 aggcccgcag cataggcgcc cgcgcgccgg ggcggcggtc gggtgcgagt ccctgtgctc
     5281 gggccaggga catccagtgt caccgggcgg cgcgaggtcg cccggcgcca aagggccact
     5341 taatgcgccg tttggcgccg ggcagttttc cgccgcgtcc ccagaatcga tggacgacct
     5401 cgcggcatgc agcgaggccg acaccacacc gggatcatgc cgaaccgacg cctatccgcc
     5461 accctcgtcc tgctcgccag cgccctgctc gaagtcgcgc tgcgcctgcg caagcgcccc
     5521 tgcatgcacg gccgcggtcg gcccctcgcg ctcggctggc tcgtcgcgct cgccacggtg
     5581 gccggggcgg caacgtcgag gccgcccgac gcgccgccca t
//
LOCUS       DASVLN010000709         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_521683, whole genome shotgun sequence.
ACCESSION   DASVLN010000709 DASVLN010000000
VERSION     DASVLN010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521683"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VF453_16745"
     CDS             <1..1207
                     /locus_tag="VF453_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX7689363.1"
                     /translation="AASAPATDAAKPDRKEARAARKAAKAQPKGVPPAKIQQAAQAAP
                     ATTARPGHVLPDRQFDIPSLKAMNADVGVKLAVLDLGTTALEPFTPLQARITLQDGVL
                     RLSDILARTSQGQVTGGIGLDSRPALPAWNADLRWSGIQLARFVKTPDTMVKRNADGS
                     RPDTGYVSGALGGDAKLTGTGKSTAALLGSLQGTARLWVQDGEVSHLLVEAAGIDIAD
                     ALGLFVKGDDKVPMQCAVAKLDIVKGKVLPEVFMIDTHASTLLVGGDLSLKDETLDLQ
                     LTSHPHNFSPWAPHGFSIVAPRTPVDVDGTFSDPHVHLEAKPLLVRGGAALALGTLVN
                     PLAALLALVDLRQPEKDVCSGAMKHLAESDRPGAVAPAASSAAPSRARAAEAAASNAR
                     HAHRKAAAH"
     gene            1275..>2515
                     /locus_tag="VF453_16750"
     CDS             1275..>2515
                     /locus_tag="VF453_16750"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7689364.1"
                     /translation="MLTATLLLLGVVLLAMCLLERHVTRLPLSPAAVYLAVGWIAGEM
                     AKSAVRVPPTAHERADMLVVVTETAVLISLFAVGLRLRTPLTLKGWRVAGLLAGPGML
                     WTVALATLAGAWLFPGLGWVGALLLAAILAPTDPVLASEVQIRSASDRDAVRLALTAE
                     GGLNDGTASPLVLLALGLAGVGALGPFGLRWGWHGLVWPIAGGVLLGALFGRVLGVTV
                     HALLRRGHGLGWDELLYLGIITLAYGLSRLTQTSSFLFVLTAAMGVFRLSPQRSDALA
                     RDGVDGPALAKRLLAFGHRCERLVEVAMVLMLGFAMAWVTLSWQQLAYGLLLIVVVRP
                     ASVLLTVPRGRMTRAQRRVVAWFGIRGVGSLFYLAQAIDAGIAPPLASALVEATLPAI
                     ALSIVLHGVSATPLMALYRRR"
BASE COUNT          289 a          939 c          926 g          361 t
ORIGIN      
        1 cgccgcgtcg gcgcccgcga ccgatgccgc gaagcccgat cgcaaggagg ccagggccgc
       61 aaggaaggcg gccaaggcgc agcccaaggg cgtgccgccc gcgaagatcc agcaggcggc
      121 ccaggccgcg ccggcgacca ccgccaggcc gggtcacgtg ctgcccgacc gccagttcga
      181 catcccgtcg ctgaaggcga tgaacgcgga cgtgggcgtg aagctcgcgg tcctcgacct
      241 cggcacgacc gcgctcgagc cgttcacgcc gctgcaggcg cgcatcacgc tgcaggacgg
      301 cgtgctgcgc ctgagcgaca tcctcgcgcg cacgtcgcaa ggccaggtca ccggcgggat
      361 cggcctggac agccgccccg ccctgcccgc ctggaacgcc gacctgcgct ggtcgggcat
      421 ccagctcgcg cgcttcgtca agacgcccga cacgatggtc aagaggaacg ccgacggctc
      481 gcgcccggac accggctacg tcagcggcgc gctcggcggc gacgccaagc tcacgggcac
      541 cggcaagtcg acggcggcgc tgctcggctc gctgcagggc acggcgcggc tgtgggtgca
      601 ggacggcgag gtctcgcacc tgctggtcga ggccgcgggc atcgacatcg ccgatgcgct
      661 gggcctgttc gtcaagggcg acgacaaggt gccgatgcag tgcgcggtcg cgaagctgga
      721 catcgtcaag ggcaaggtgc tgcccgaggt cttcatgatc gacacccatg cctcgacgct
      781 gctggtcggc ggcgacctgt cgctgaagga cgagacgctg gacctccagc tgacctccca
      841 tccgcacaac ttctcgccat gggcgccgca cggcttctcg atcgtcgcgc cgcgcacgcc
      901 ggtggacgtc gacggcacgt tctccgaccc gcacgtgcac ctcgaggcca agccgctgct
      961 ggtgcgcggc ggggcggcgc tggcactcgg gacgctggtc aatccgctcg cggcgctgct
     1021 ggcgctcgtc gacctgcgcc agcccgagaa ggacgtctgc agcggtgcga tgaagcacct
     1081 ggcggagtcc gaccgtcccg gcgccgtcgc gcctgcggcg tcctcggccg cgccctcgcg
     1141 cgcccgggcc gccgaggccg cagcctccaa cgcgcgacac gcgcaccgca aggccgcggc
     1201 ccattgaccc gccgccgccc gccggcccgc tcgcgctcgc gccgcgggcc gcctccttcg
     1261 acgatcgcca cgccatgctg accgccacgc tgctgctgct gggcgtcgtg ctgctggcca
     1321 tgtgcctgct cgagcgccac gtcacgcgcc tgccgctgtc cccggctgcg gtctacctgg
     1381 ccgtcggctg gatcgccggc gagatggcga agtccgcggt gcgcgtgccg cccaccgcgc
     1441 acgagcgcgc cgacatgctg gtcgtcgtga ccgagacggc ggtgctgatc tcgctgttcg
     1501 ccgtgggatt gcgcctgcgc acgccgctca cgctcaaggg ctggcgcgtg gcgggcctgc
     1561 tggccggccc cggcatgctg tggacggtgg cgctggcgac gctggcgggc gcctggctgt
     1621 tcccgggact cggctgggtc ggcgcgctgc tgctggcggc catcctcgcg ccgacggatc
     1681 cggtgctcgc gtccgaggtg cagatccgct cggccagcga ccgcgacgcc gtgcggctcg
     1741 cgctgaccgc cgagggcggc ctcaacgacg gcacggcctc gccgctggtg ctgctcgcgc
     1801 tgggcctggc gggcgtcggc gcgctcggcc ccttcgggct gcgctgggga tggcacggcc
     1861 tcgtctggcc gatcgccggc ggcgtgctgc tgggggccct gttcggtcgc gtcctgggcg
     1921 tgacggtgca cgcgctgctg cgccgcggcc acgggctggg ctgggacgag ctgctgtacc
     1981 tgggcatcat cacgctcgcc tacggactgt cgcgactgac gcagacctcc tcgttcctgt
     2041 tcgtgctgac ggcggcgatg ggggtgttcc gcctgtcgcc gcagcgcagc gacgcgctcg
     2101 cccgcgatgg tgtcgacggc ccggcgctcg ccaagcggct gctcgcgttc gggcatcgct
     2161 gcgaacggct cgtcgaggtc gcgatggtgc tgatgctcgg cttcgcgatg gcctgggtga
     2221 cgctgagctg gcagcagctc gcctacggcc tgctgctgat cgtcgtcgtg cggccggcga
     2281 gcgtgctgct caccgtcccg cgcgggcgca tgacgcgcgc gcagcgacgc gtcgtggcgt
     2341 ggttcgggat ccgcggcgtc ggctcgctgt tctacctcgc gcaggcgatc gacgccggca
     2401 tcgcgccgcc gctggccagc gcgctcgtcg aggcgacgct gcccgccatc gcgctgtcca
     2461 tcgtgctgca cggcgtctcg gcgacgccgc tgatggcgct gtaccggcgg cgcag
//
LOCUS       DASVLN010000710         5393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_306554, whole genome shotgun sequence.
ACCESSION   DASVLN010000710 DASVLN010000000
VERSION     DASVLN010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306554"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..825
                     /gene="ccmB"
                     /locus_tag="VF453_16755"
                     /pseudo
     CDS             155..825
                     /gene="ccmB"
                     /locus_tag="VF453_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
     gene            963..1700
                     /gene="ccmC"
                     /locus_tag="VF453_16760"
     CDS             963..1700
                     /gene="ccmC"
                     /locus_tag="VF453_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEX7689365.1"
                     /translation="MTWFTFAAPANFYRLAGRLVPAFAIVAAPFALAGLWMGFFVAPS
                     DAVQGEVYRIIFIHVPAAWLSMVMYVAMAGWAALGWLLNARMAFILARAIAPSGALFT
                     LLALWTGSLWGRPTWGTWWVWDARLTSELILLFLYLGYMALVSAIDDVRRADKAGALL
                     AIVGVVNVPIIYFSVVWWNTLHQGSSLTMGAQPRMAAPMLEAMLLMTVACWAWAFAVV
                     FMRARALVLEREADTAWARALVAGKRT"
     gene            1704..1910
                     /gene="ccmD"
                     /locus_tag="VF453_16765"
     CDS             1704..1910
                     /gene="ccmD"
                     /locus_tag="VF453_16765"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX7689366.1"
                     /translation="MHWQDANDFFSMGGRGGFVWGAYGVMAVLLLAEALLVHRRHRAA
                     RRAIAERLEDEAAARTAATPEQGS"
     gene            1907..2356
                     /gene="ccmE"
                     /locus_tag="VF453_16770"
     CDS             1907..2356
                     /gene="ccmE"
                     /locus_tag="VF453_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX7689367.1"
                     /translation="MTPRRKRLAIIGGLLAAVVAITALVLNAFRSNLVFFYSPTQVAQ
                     HEAPQGRTFRIGGMVVKGSLQRDGLDCRFTVTDFARSMTVHYRGLLPDLFKEGKGVVA
                     QGRLGTDGVFDAQEVLAKHDENYMPPEAAAALKAAQKGGTMKTEANR"
     gene            2353..4365
                     /locus_tag="VF453_16775"
     CDS             2353..4365
                     /locus_tag="VF453_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX7689368.1"
                     /translation="MIPELGQVALLLALAVALILGTLPLAGAALGRDSWMALARPAAR
                     AHGLLVAVAFACLVACFVRNDFSVLYVASNSNTSLPIWYRIAGVWGGHEGSLLLWLAM
                     LSVWMIAVAQFSRRLPDPFVARVLGVMGLVSIGFLLFMLTTSNPFDRLVPVPLDGNDL
                     NPLLQDPGMVAHPPMLYMGYVGFSVAFAFAVAALIEGRLDAAWARWTRPWTTAAWCCL
                     TIGIMLGSMWAYYVLGWGGWWFWDPVENASFMPWLAGTALIHSLAVTEKRGAFKSWTV
                     LLALLAFTLSLLGTFIVRSGVLSSVHAFATDPKRGLFILVFLGIVVGAALTLYAWRAP
                     SVGLGARFSLVSRESMLLGNNVLFVVVCATVLLGTMYPLLLDALGMGKISVGPPYFEA
                     VFVPLMVPVVALLALGPLARWKQASLPSLLHRLRWAALAAVLGALATGLVAGRISPVA
                     TLGFLMAYWIVASIAVDALERLRAENHAGTPVLARVRRFPRALVGMWLAHLGVAAFAF
                     GVSMVRTYEVEQDLTMSFGSSAEVGGYRFQLDELHEVHGPNYEALQATMPVTRDGRQV
                     AVLHPEKRVYRVQRTPTTEAAIQPGVTRDLYVSLGEMVKPDTWTLRIHVKPFVDWIWG
                     GCLLMAFGGAVALSDRRYRRGARRTQDEDAGTGLPVAARPATEVAA"
     gene            4362..4895
                     /locus_tag="VF453_16780"
     CDS             4362..4895
                     /locus_tag="VF453_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX7689369.1"
                     /translation="MKASLRFAIPLGIFAALVAFCAVGLHLDPREVPSPLIDKPAPEF
                     ALPRLDAPGNTIRREDMLGKVWMLNVWASWCDSCREEHPWLVEFAKTKAVPIYGLDYK
                     DQREPALAWLAERGNPYEASLFDASGRTGIDFGVYGVPETFVIDKQGVIRFKQIGALT
                     PDVLQNKVLPLLRRLNG"
     gene            4888..>5393
                     /locus_tag="VF453_16785"
     CDS             4888..>5393
                     /locus_tag="VF453_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX7689370.1"
                     /translation="MADRRLFAAIALALAVAGVGARAQAAPSAPEAAASAATVPFAPP
                     KAVPTASDPAVEARMIAITSELRCLVCQNQTIADSHADLAVDLRQEVREMIRAGRSDD
                     QIRHYMTDRYGDFVLYRPPFKASTLALWLGPLALLVVAVVALVAAISRRARLADDRFE
                     PDPAWDDDA"
BASE COUNT          606 a         1960 c         1953 g          874 t
ORIGIN      
        1 gacctgccgg cgacgctgcg ggacgacgag gcgatggccg catgaggctc cacccggccg
       61 ctccgacagg ggctcgcatc gcgcggcgcg gcacggcggt ccgctttgcg cgcggcgtcc
      121 gaaccgccgc gtcgatccgg ccggaggccc gcccgtgaac gcggtcgtct tcgccgcgac
      181 ctatcgacgc gacctgcgcc tggcctggcg ccggcgcacc gaggcggcgc tgccgctcgc
      241 gttcttcctc gtcgccgcca gcctgttccc gctgggcgtc gggccggagc cgcaggtgct
      301 cgcgcagatc gcgccgggcg tcgtctgggt gtgcgcgctg ctggcggcca tgctgtccat
      361 gccgccgatg tacgccgccg accacgccga cggctcgctg gagcagctgc tgctcgcgcc
      421 gggcgccggg ctctccgtcg ccggggccaa ggccgccgcg cactggacga tcagcggcgc
      481 gccgctggtc gtcggcgcgc cgttgctggg gctgctgttc gggctggcgc cgcgggaact
      541 cggggtgctc gccgcgtctc tactgctggg acgccggtgc tcagcctcct cggcgggctg
      601 ggcgcggcgc tcaccctcgg gctgcgcagc gcgggcatgc tcatcgtgct gatcgtgctg
      661 ccgctggcgg tgccggcgct gatcttcggc gccggggcgg cggcggcggt cgacgcgggc
      721 ctgtcggctt cgccgcacct gtcgctgctg ggggcgctgc tgatcgcctg cacgctcggc
      781 gcgccgccgg ccaccgcggc ggcgctgcgc atcgcgctcg agtgagccgc ggcggcccgg
      841 cgcaaacatt tatgacattg ccgaatgtcg agcatcaacc cgccgccccg aactcgccgg
      901 acaatcacgt cgtgaccacg ccgctcgccc ccgccgccga tcgtccgcgc caggccgccc
      961 gcctgacgtg gttcaccttc gccgcgcccg cgaacttcta tcgcctggcc ggccggctgg
     1021 tgcccgcgtt cgcgatcgtc gccgcgccgt tcgcgctcgc cgggctgtgg atgggcttct
     1081 tcgtcgcacc gagcgacgcg gtgcagggcg aggtctaccg catcatcttc atccacgtgc
     1141 cggcggcctg gctgtcgatg gtgatgtacg tcgcgatggc gggctgggcg gcgctcggct
     1201 ggctgctgaa cgcgcgcatg gctttcatcc tcgcgcgcgc catcgcgccc agcggggcgc
     1261 tgttcacgct gctcgcgctc tggacgggct cgctgtgggg ccggccgacc tggggcacct
     1321 ggtgggtgtg ggacgcgcgg ctcacgtcgg agctgatcct gctgttcctc tacctcggct
     1381 acatggcgct ggtctcggcg atcgacgacg tgcgccgcgc cgacaaggcg ggcgcgctgc
     1441 tggcgatcgt cggcgtggtc aacgtgccga tcatctattt ctcggtggtg tggtggaaca
     1501 cgctgcacca gggctccagc ctgacgatgg gcgcgcagcc ccgtatggcg gcgccgatgc
     1561 tggaggcgat gctgctgatg acggtcgcct gctgggcctg ggcgttcgcc gtggtcttca
     1621 tgcgcgcgcg cgccctcgtg ctggagcgcg aggccgacac cgcctgggcg cgcgcgctcg
     1681 tcgccggaaa gaggacctga gccatgcact ggcaggatgc gaacgacttc ttctcgatgg
     1741 gcggccgcgg cgggttcgtc tggggcgcct acggcgtgat ggccgtgctg ctgctggccg
     1801 aggcgcttct cgtgcatcgc cgccatcgcg ccgcgcgccg ggcgatcgcc gaacgtctcg
     1861 aggacgaggc cgcggcgcgc accgccgcga cgccggagca gggatcatga ccccgcgccg
     1921 caagcgcctc gcgatcatcg gcggcctgct ggccgccgtc gtcgccatca cggcgctggt
     1981 gctcaacgca ttccgctcga acctggtgtt cttctactcg cccacgcagg tggcgcagca
     2041 cgaggcgccc cagggccgca ccttccgcat cggcggcatg gtggtcaagg gcagcctgca
     2101 gcgcgacggc ctggactgcc gcttcacggt caccgacttc gcgcggtcga tgacggtgca
     2161 ctaccgcggc ctgctgcccg acctgttcaa ggagggcaag ggcgtggtcg cgcagggccg
     2221 gctcggcacg gacggcgtgt tcgacgccca ggaagtgctc gccaagcacg acgagaatta
     2281 catgccgccc gaggccgcgg ccgcgctcaa ggcggcccag aagggcggca cgatgaagac
     2341 ggaggcgaac cgatgatccc cgaactcggc caggtcgcgc tgctgctcgc gctggcggtg
     2401 gcgctgatcc tgggcaccct gccgctggcc ggcgcggcgc tcgggcgcga cagctggatg
     2461 gcgctcgcgc gcccggccgc gcgcgcgcac gggctgctgg tggcggtcgc cttcgcgtgc
     2521 ctcgtcgcct gcttcgtgcg caacgacttc tcggtgctct acgtcgcgag caactccaac
     2581 acctcgctgc cgatctggta ccgcatcgcg ggcgtctggg gcggccacga gggttcgctg
     2641 ctgctgtggc tggcgatgct gagcgtctgg atgatcgcgg tggcgcagtt cagccgccgc
     2701 ctgcccgacc cgttcgtcgc gcgggtgctg ggcgtcatgg gcctggtgag catcggcttc
     2761 ctgctgttca tgctgacgac gtccaacccg ttcgaccgcc tcgtgccggt cccgctggac
     2821 ggcaacgacc tgaacccgct gctgcaggat cccggcatgg tggcgcatcc gccgatgctg
     2881 tacatgggct acgtcggctt ctcggtggcg ttcgcgttcg ccgtcgccgc actgatcgag
     2941 ggccgcctgg acgccgcctg ggcgcgctgg acgcgcccgt ggacgaccgc cgcgtggtgc
     3001 tgcctgacca tcggcatcat gctgggcagc atgtgggcgt actacgtcct cggctggggc
     3061 ggctggtggt tctgggatcc ggtggagaac gcgtcgttca tgccctggct cgcgggcacc
     3121 gcgctgatcc actcgctggc ggtgaccgag aagcgcggcg ccttcaagtc gtggaccgtg
     3181 ctgctcgcgc tgctcgcgtt cacgctgtcg ctgctgggca cgttcatcgt gcgctcgggc
     3241 gtgctgtcgt cggtgcacgc gttcgcgacc gacccgaagc gcggcctgtt catcctcgtg
     3301 ttcctcggca tcgtcgtcgg cgcggcgctg acgctctacg cctggcgcgc gccgagcgtc
     3361 ggcctgggcg cccggttctc gctcgtgtcg cgcgagtcca tgctcctggg caacaacgtg
     3421 ctgttcgtgg tggtgtgcgc gacggtgctg ctcggcacga tgtacccgct gctgctggac
     3481 gcgctgggca tgggcaagat ctcggtcggg ccgccgtact tcgaggccgt cttcgtgccg
     3541 ctgatggtgc cggtcgtcgc gctgctggcg ctcgggccgc tcgcgcgctg gaagcaggcc
     3601 tcgctgccgt cgctgctgca ccggctgcgc tgggcggcgc tcgcggcggt gctgggcgcg
     3661 ctggccaccg gcctggtcgc cgggcgcatc tcgccggtcg cgacgctcgg cttcctcatg
     3721 gcgtactgga tcgtcgcgtc catcgccgtc gacgccctcg agcgcctgcg cgccgagaac
     3781 catgccggca cgccggtcct cgcccgcgtg cggcgctttc cgcgcgcgct ggtgggcatg
     3841 tggctcgcgc acctgggcgt ggcggcgttc gcgttcgggg tcagcatggt gcgcacctac
     3901 gaggtcgagc aggacctgac gatgtccttc ggcagcagcg ccgaggtcgg cggctaccgc
     3961 ttccagctcg acgagctgca cgaggtgcac ggccccaact acgaggcgct gcaggcgacg
     4021 atgccggtca cgcgcgacgg ccgccaggtc gccgtgctgc atccggagaa gcgcgtctac
     4081 cgcgtccagc gcacgcccac caccgaggcc gcgatccagc cgggcgtgac gcgcgacctc
     4141 tacgtctcgc tcggcgagat ggtcaagccc gacacctgga cgcttcgcat ccacgtcaag
     4201 cccttcgtcg actggatctg gggcggctgc ctcctcatgg ccttcggcgg cgccgtggcg
     4261 ctgagcgacc gccgctaccg ccgcggcgcg cgccgcacgc aggacgagga cgccggcacc
     4321 ggattgccgg tcgcggcgcg tcccgccacg gaggtcgcgg catgaaggcg tcgctgcgat
     4381 tcgccatccc gctggggatc ttcgccgcgc tcgtcgcgtt ctgcgcggtc ggcctgcacc
     4441 tcgacccgcg cgaggtgccc tcgccgctga tcgacaagcc cgcgccggag ttcgcgctgc
     4501 cgcggctcga cgcgcccggc aacacgatcc ggcgcgagga catgctcggc aaggtctgga
     4561 tgctcaatgt ctgggcctcg tggtgcgaca gctgccgcga ggagcatccc tggctcgtcg
     4621 agttcgcgaa gaccaaggcg gtgccgatct acggcctcga ctacaaggac cagcgcgagc
     4681 ccgcgctggc ctggctcgcc gagcgcggca acccctacga agcgtcgctg ttcgacgcca
     4741 gcggccgcac cggcatcgac ttcggcgtct acggcgtgcc cgagaccttc gtgatcgaca
     4801 agcagggcgt gatccgcttc aagcagatcg gcgcgctcac gcccgacgtg ctgcagaaca
     4861 aggtgttgcc gctgctgcgg aggctcaatg gctgaccggc gcctgttcgc tgcgatcgcg
     4921 ctggcgctcg cggtcgcagg tgtgggtgcg cgcgcgcagg cggccccatc cgcgccggag
     4981 gctgcggcct ccgcggcgac ggtgcccttc gcgccgccga aggccgtgcc gacggcctcc
     5041 gatcccgccg tcgaggcgcg catgatcgcg atcacgagcg agctgcgctg cctggtctgc
     5101 cagaaccaga cgatcgccga ctcgcacgcc gacctcgccg tcgacctgcg ccaggaagtg
     5161 cgcgagatga tccgcgccgg ccgcagcgac gaccagatcc gccactacat gaccgaccgc
     5221 tacggcgact tcgtgctgta ccggccgccg ttcaaggcgt cgacgctcgc gctgtggctc
     5281 gggccgctgg cgctgctggt cgtcgccgtc gtcgcgctgg tcgccgcgat ctcgcgccgc
     5341 gcgcgcctgg ccgacgaccg cttcgagccc gacccggcct gggacgacga cgc
//
LOCUS       DASVLN010000711         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_90434, whole genome shotgun sequence.
ACCESSION   DASVLN010000711 DASVLN010000000
VERSION     DASVLN010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90434"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VF453_16790"
     CDS             <1..1030
                     /locus_tag="VF453_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7689371.1"
                     /translation="AQPAIRTAPAAGSFSAAVRRAAPAVVSITASRASVRRNDEDAWQ
                     RYFFGGGAGNPAQTGLGSGVLVSSEGYLLTNQHVVDGADDIEVTLADGRSASAQIVGT
                     DADSDLALLRIPLENVPVIPFGDIAQLAVGDPVLAIGNPFNVGQTVTSGIVSALDRNQ
                     LGLSAIENFIQTDAAINPGNSGGALVDGQGRLVGINTAIFSRSGGSMGIGFAIPVDIA
                     REVMDSLIRDGHMTRGWIGVQPRDLTPEFADSFQLPVHQGVLVTGVLRDGPAAAAGLK
                     PGDVVTAIGGVKVTNTAQLLRAVGALQPPARARLDVQRGSDALHLDVPVANRPPRPRG
                     TRDADDAR"
     gene            complement(1052..1840)
                     /gene="tatC"
                     /locus_tag="VF453_16795"
     CDS             complement(1052..1840)
                     /gene="tatC"
                     /locus_tag="VF453_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX7689372.1"
                     /translation="MTDPADELAGTEQPFVAHLVELRDRLIRSLYGIGLAFVALAVWP
                     GPRHLYDLLAQPLIAALPAGSHMIAIGVVTPFLIPIKVTVLVAFGIALPWVLYQVWAF
                     VAPGLYRHEKRLVLPLVVTSTILFYLGVAFCYFFVFQKAFPAIQRMAPASVQLAPDIE
                     AYLDFVLTMFIAFGVAFEVPVAVVIVARLGLVTVAQLRKFRAYYMVAAAGVAGVVTPP
                     DPASMIALLLPMLLLYEVGIWAVPVFIRTTQSPEAAQDAKSNGS"
BASE COUNT          301 a          688 c          720 g          228 t
ORIGIN      
        1 cgcgcagccg gcgatccgca cggctccggc ggccggcagc ttctccgccg ccgtgagacg
       61 cgcggcgccc gccgtcgtca gcatcaccgc gagccgcgcg agcgtgcgcc gcaacgacga
      121 ggacgcctgg cagcgctact tcttcggcgg cggcgcgggc aaccccgcgc agacgggcct
      181 gggctcgggc gtgctcgtct cgagcgaggg ctacctgctc accaaccagc atgtcgtcga
      241 cggcgcggac gacatcgagg tcacgctggc ggacggccgc tccgcgagcg cgcagatcgt
      301 gggcaccgat gccgactccg acctcgcgct gctgcgcatc ccgctcgaga acgtgccggt
      361 gatcccgttc ggcgacatcg cgcagcttgc cgtgggcgac ccggtgcttg cgatcggcaa
      421 ccccttcaac gtcggccaga ccgtgacgtc gggcatcgtc agcgcgctcg accgcaacca
      481 gctcgggctg tcggcgatcg agaacttcat ccagaccgac gcggcgatca accccggcaa
      541 ctcgggcggc gcgctggtcg acggccaggg ccggctggtg ggcatcaaca cggcgatctt
      601 ctcgcgcagc ggcggcagca tgggcatcgg cttcgccatc ccggtcgaca tcgcgcgcga
      661 ggtgatggac agcctgatcc gcgacggcca catgacgcgc ggctggatcg gcgtgcagcc
      721 gcgcgacctc acgccggagt tcgcggacag cttccagctg ccggtgcacc agggcgtgct
      781 cgtgacgggc gtgctgcgcg acggcccggc cgcggcggca ggcctgaagc ccggcgacgt
      841 cgtcaccgcg atcggcgggg tgaaggtgac caacaccgcg cagctgctgc gcgcggtcgg
      901 ggcgctccag ccgccggcgc gggcccggct ggacgtccag cgcggcagcg acgcgctgca
      961 cctggacgtg ccggtcgcca accggccgcc gcgaccgcgc ggcacgcgcg acgccgacga
     1021 cgcgcgctga cccgaaggct cccgggcgcg ctcaggagcc gttggacttc gcgtcctgcg
     1081 ccgcctcggg cgactgcgtc gtgcggatga agaccggcac cgcccagatg ccgacctcgt
     1141 agagcagcag catcggcagg agcagcgcga tcatcgaggc cggatccggc ggcgtcacca
     1201 cgcccgcgac gccggcggcg gcgaccatgt agtacgcgcg gaacttgcgc agctgcgcga
     1261 ccgtcaccag cccgagccgc gcgacgatca cgaccgccac cggcacctcg aaggcgacgc
     1321 cgaaggcgat gaacatcgtc agcacgaagt ccaggtaggc ctcgatatcg ggggcgagct
     1381 gcaccgacgc cggcgccatg cgctggatcg cggggaaggc cttctggaac acgaagaagt
     1441 agcagaaggc gacgccgagg tagaacagga tcgtgctcgt gaccacgagc ggcagcacga
     1501 gccgcttctc gtgccggtag agccccggcg cgacgaaggc ccacacctgg tacagcaccc
     1561 agggcagcgc gatgccgaag gcgaccagca cggtgacctt gatcgggatc aggaagggcg
     1621 tcacgacgcc gatcgcgatc atgtgcgacc ccgcgggcag cgccgcgatc agcggttgcg
     1681 caagcaggtc gtagagatgg cgcgggcccg gccacaccgc gagcgcgacg aaggcgaggc
     1741 cgatgccgta gaggctgcgg atcaggcggt cgcgcagctc caccaggtgg gcgacgaagg
     1801 gttgctcggt gccggccagt tcgtcggcgg ggtccgtcat ctcatgggct ccgcggcgcg
     1861 tcggcgccgt gttgtcgctg cggggggggg tggcgccgcc ggtcacggct ggcgcgagcg
     1921 gccggcgggg cggaagc
//
LOCUS       DASVLN010000712         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_35996, whole genome shotgun sequence.
ACCESSION   DASVLN010000712 DASVLN010000000
VERSION     DASVLN010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35996"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VF453_16800"
     CDS             <1..321
                     /locus_tag="VF453_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7689373.1"
                     /translation="TLDASFLAARRHTGHVVSALGWGTHALAPLSFRGATYSGIFTLL
                     PMLTGEGRIHHGEILEAVARIVDGGALRPRVASTRLGLADAAAAHELVAGGRARGKVV
                     VEIA"
     gene            complement(322..957)
                     /locus_tag="VF453_16805"
     CDS             complement(322..957)
                     /locus_tag="VF453_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7689374.1"
                     /translation="MTSPSRLAEPHLPPAAPTAAVRILEQRVLADDWYVLRKTTFEIT
                     RRDGRAQVVSRETYDRGNGAVLLLFDPARGTVVLTRQFRFPAYVNGCADGLLVEACAG
                     LLDGQAPEEAIRREVAEETGYAVRAPRKVFEAYMSPGSVTEKLHFFVAAIDAADRAGA
                     GGGDESEGEDIEVLELPLAEALAMVGRGEIEDGKTIMLLQHAALVGLDRLG"
     gene            1051..1806
                     /locus_tag="VF453_16810"
     CDS             1051..1806
                     /locus_tag="VF453_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7689375.1"
                     /translation="MLTTQRKKLLLDRLAAEGQLVAKALARELGTSEDTIRRDLRELA
                     AEGRLQRVHGGALPASAALGDLEAREQVAIEDKIALGRFGAGLVHPGQVVILDGGTTA
                     VQVARHLAPGLRATVVTHSPPVAVALAAHPGVEIIVLGGRLFRHSMVNVGAAVIAAAG
                     RLRADLFLLGVTGVHPQEGLTTGDAEEAAVKRALHERAAETVVLASQEKLLAASAFLV
                     APLSSASLVAVPRATPERTVRALRAGGVKVQRA"
BASE COUNT          222 a          668 c          685 g          246 t
ORIGIN      
        1 acgctggacg cgtccttcct cgccgcgcgg cgccacaccg gccacgtcgt gagcgcgctc
       61 ggctggggca cgcacgcgct ggcgccgctg tcgttccgcg gcgcgacgta ttcgggcatc
      121 ttcacgctgc tgccgatgct caccggcgag ggccgcatcc accacggcga gatcctggag
      181 gccgtcgccc gcatcgtcga tggcggcgcg ctgcgcccgc gcgtggcgtc gacccgcctc
      241 ggcctggccg acgccgcggc cgcgcacgag ctggtggccg gcgggcgcgc gcgcggcaag
      301 gtcgtggtcg agatcgcctg atcagccgag gcggtcgagg ccgaccaggg ccgcgtgctg
      361 cagcagcatg atcgtcttgc cgtcctcgat ctcgccgcgg ccgaccatcg ccagggcctc
      421 ggccaggggc agctcgagga cctcgatgtc ctcgccctcc gactcgtcgc cgccgccggc
      481 gcccgcgcgg tccgcggcgt cgatcgcggc gacgaagaag tgcagcttct cggtcacgga
      541 gccggggctc atgtacgcct cgaagacctt gcgcggcgcg cgcaccgcgt agccggtctc
      601 ctcggcgacc tcgcgccgga tcgcctcctc gggcgcctgg ccgtccagca gcccggcgca
      661 ggcctcgacc agcagtccgt ccgcgcagcc gttgacgtag gccgggaagc ggaactggcg
      721 cgtgagcacc accgtgccgc gagccgggtc gaacagcagc agcaccgcgc cgttgccgcg
      781 gtcgtaggtc tcgcggctca cgacctgcgc gcggccgtcg cggcgggtga tctcgaaggt
      841 cgtcttgcgc agcacgtacc agtcgtcggc gagcacgcgc tgctcgagga tgcgcacggc
      901 cgcggtcggc gcggcgggcg gcaggtgcgg ttcggccagg cggctcggcg atgtcatggc
      961 gaagtcgggt agtttcgtgc agtatcgtgc aatcaaccga tcaccgcaac tgcatgagac
     1021 tgcacgaaac ggcatgcgat gatcgccgcg atgctcacga cccagcgcaa gaagctcctg
     1081 ctcgatcgcc tcgccgccga gggccagctc gtggccaagg cgctggcgcg cgagctcggc
     1141 acctccgagg acaccattcg ccgcgacctg cgcgagctcg ccgccgaagg ccgtctgcag
     1201 cgcgtgcacg gcggcgcgct gccggcttcg gcggcgctgg gcgacctcga ggcgcgcgag
     1261 caggtggcga tcgaggacaa gatcgcgctc ggccgcttcg gcgccggcct ggtgcacccg
     1321 gggcaggtgg tgatcctcga cggcgggacg accgcggtgc aggtcgcgcg ccatctcgcg
     1381 cccggcctgc gcgccacggt cgtcacgcac agcccgccgg tggcggtggc gctggccgcg
     1441 catccgggcg tcgagatcat cgtgctcggc ggccggctgt tccgtcattc gatggtcaac
     1501 gtcggcgccg cggtgatcgc ggcggcgggg cggctgcgcg ccgacctgtt cctcctcggc
     1561 gtgactggcg tgcatccgca ggagggcctg acgaccggcg acgccgagga ggccgccgtc
     1621 aagcgcgcgc tgcacgagcg cgcggccgag accgtcgtgc ttgcctcgca ggagaagctg
     1681 ctcgccgcct cggccttcct cgtcgccccg ctgtcgtcgg cgagcctggt cgcggtgccg
     1741 cgggcgacgc ccgagcgcac ggtgcgggcg ctgcgggcgg gcggcgtgaa ggtgcagcgc
     1801 gcatgaggac gcgagcggca c
//
LOCUS       DASVLN010000713         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_468709, whole genome shotgun sequence.
ACCESSION   DASVLN010000713 DASVLN010000000
VERSION     DASVLN010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468709"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="VF453_16815"
     CDS             complement(<1..1638)
                     /locus_tag="VF453_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX7689376.1"
                     /translation="MSHAPLPTPSFDRFPRHEELGALLRAYADARPDLVAVRSIGRSH
                     EGREIWVATLTNTATGDDVDKPALWVDGNIHAAELTACTAALYYLHQLASSHGTDARV
                     THLLDTRAVYLCPRLNPDGAELALAERPRHIRSSTRPYPFDEDHVDGMVLEDVDGDGR
                     ILYMRVPDPHGTYKKHPCEPRLMVAREPGEVGGEYFRVMPEGTIKDWDGLRLRLNRDR
                     EGLDLNRNFPSQWRQEFEQVGAGPYPANEPEVRAMVDFCVAHPNIGAGISFHTHSGVI
                     LRPMGTTSDDDMVPEDLWMIKRLSEIGSKLTGYPALSVFHDFKYHPKEVVTGTQDWLY
                     EHLGALCWTVEIWAPNREAGIEKYDYIHWYRDHPPEDDLKLLKWSDEQCGGQAHVDWR
                     PFVHPQLGPVEIGGWDRMNYWRNPPPHLREREAARFPAWLTTLGLALPKLEVLQASCE
                     PLGHDGQGDTWRLRLAVGNAGYLPAYVSQRAKTRKVVRGTVFEIALPAGATLVTGKAR
                     ELGEHLEGHAPRGTLQAFLPDRAITADRTLQEWIVRGP"
     gene            complement(1687..3681)
                     /locus_tag="VF453_16820"
     CDS             complement(1687..3681)
                     /locus_tag="VF453_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX7689377.1"
                     /translation="MQEAVPSRSELTLRGVLLGFLITLLFTAANVYFGLKAGLTFATS
                     IPAAVISMGLLRRARDVSIQENNIVQTVASAAGAISSIIFVLPGLVMVGWWNGFPYLT
                     CVVICALGGVLGVTYSIPLRRALVTTSDLPYPEGVACAEVLKVGADTGAGAAEENRAG
                     LLAIVWGGVASAAFQLVVGTQLFAADLAGYFRLSRSSNASTGVDLGLSMALLAVGHLV
                     GLWVGLAMLAGLLIAWAGAVPELTAMLPFDAGADVAAIATHAWAKQVRFIGAGAIAVA
                     AVWTLVKLVRPVAKGLASAMAASRVRRAGDGAALPRTEQDLPIGWVAGVTLACLVPVG
                     WLLADMTSGAGLGSAAVVLVAGGIAFIALMSFFVAAVCGYMAGLIGSSNSPLSGVGII
                     VVISAALLVAAVAKPMLPASAGPALVAFTLFVTSVVVAAATVANDNLQDLKTGQLVDA
                     TPWKQQVALAIGTVAGALVIPPVLDLLQRAYGFAGAPGATAKALAAPQAALISALAKG
                     VLGGDLDWNLLGKGVALGVGLVVLDEAIKRWRKPGSHAQLPPLAVGLGIYLPMSTTLM
                     VVVGAVVGWAWDRRADASRRPASARQLGVLLASGMIVGEGLFGVAIAALAVFSGKDAP
                     LALVGDGFAGKALWLGGAGFVLATWALYRWLARMSDRVAA"
     gene            complement(3820..>4368)
                     /locus_tag="VF453_16825"
     CDS             complement(3820..>4368)
                     /locus_tag="VF453_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7689378.1"
                     /translation="LDATDRAALDAFFRDTGPVDHLVLTLSGGEGAGEFARLDLQALR
                     RGFEAKFWPQLETVQAALPALATAASITFVTAVSARIARPGTTGLAAINGALEAMVGG
                     LARELAPRRVNAVSPGVIDTAWWSRAPAAVREAVFDEQSRTLPVGRVGRPEDVAQSVL
                     FMATNGFTTGSIVECDGGLHLL"
BASE COUNT          632 a         1583 c         1601 g          552 t
ORIGIN      
        1 cgggccgcgg acgatccact cctgcagcgt gcggtcggcg gtgatcgcgc gatcgggcag
       61 gaaggcctgc agcgtgccgc gcggcgcatg gccctccagg tgctcgccga gttcgcgcgc
      121 cttgcccgtc acgagcgtcg cgccggcggg cagcgcgatc tcgaagacgg tgccgcgcac
      181 caccttgcgg gtcttggcgc gctggctcac gtaggccggc aggtagccgg cgttgcccac
      241 ggccaggcgc agccgccagg tgtcgccctg gccgtcgtgg cccagcggct cgcagctggc
      301 ctgcagcacc tccagcttcg gcagcgccag cccgagcgtc gtcagccacg ccgggaagcg
      361 cgccgcctcg cgctcgcgca ggtgcggcgg cgggttgcgc cagtagttca tgcgatccca
      421 gccgccgatc tccaccggcc cgagctgcgg gtgcacgaac ggccgccagt cgacgtgcgc
      481 ctggccgccg cactgctcgt cgctccattt cagcagcttc aggtcatcct cgggcggatg
      541 gtcgcggtac cagtggatgt agtcgtactt ctcgatgccg gcctcgcggt tgggcgccca
      601 gatctcgacc gtccagcaca gcgcgcccag gtgctcgtag agccagtcct gcgtgccggt
      661 cacgacctcc ttcgggtggt acttgaagtc gtggaacacg ctcagcgcgg gatagccggt
      721 cagcttcgag ccgatctccg acagccgctt gatcatccac aggtcctcgg gaaccatgtc
      781 gtcatccgag gtcgtgccca tcggccgcag gatcacgccg ctgtgggtgt ggaagctgat
      841 gcccgcgccg atgttcgggt gcgcgacgca gaagtccacc atcgcgcgca cctcgggctc
      901 gttggccggg taggggccgg cgccgacctg ctcgaactcc tggcgccact gcgaggggaa
      961 gttgcggttc aggtcgaggc cttcgcggtc gcggttcagg cgcaggcgca ggccgtccca
     1021 gtccttgatc gtgccctccg gcatgacgcg gaagtactcg ccgccgacct cgcccggctc
     1081 gcgcgcgacc atcagccgcg gctcgcaggg atgcttcttg tacgtgccat gcgggtcggg
     1141 gacgcgcatg tacaggatgc ggccgtcgcc gtcgacgtcc tccagcacca tgccgtcgac
     1201 atggtcctcg tcgaacgggt agggccgcgt cgacgagcgg atgtggcgcg gccgctccgc
     1261 cagcgcgagc tcggcgccgt ccgggttcag gcgcgggcag aggtagacgg cgcgggtgtc
     1321 gagcaggtgc gtgacgcgcg cgtccgtgcc gtgcgacgag gcgagctggt gcaggtagta
     1381 cagcgccgcg gtgcaggcgg tgagctcggc ggcgtggatg ttgccgtcga cccagagcgc
     1441 gggcttgtcg acgtcgtcgc cggtggcggt gttcgtcagc gtcgcgaccc agatctcgcg
     1501 gccctcgtgg ctgcggccga tcgagcgcac cgccaccagg tccggccgcg cgtcggcata
     1561 ggcccgcagc agggcgccga gctcctcgtg gcgcgggaag cggtcgaacg acggcgtggg
     1621 cagcggcgcg tgggacatgg cggcggaaag gagcgagtgg gcaatggctg gcgattctgc
     1681 gccggctcag gcggccacgc gatcgctcat ccgcgccagc cagcggtaca gcgcccaggt
     1741 cgcgagcacg aagcccgcgc cgccgagcca cagcgccttg ccggcgaagc cgtcgccgac
     1801 gagcgcgagc ggcgcatcct tgccgctgaa gaccgccagc gccgcgatgg cgacgccgaa
     1861 cagcccttcg ccgacgatca tgccgctggc cagcagcacg ccgagctgcc tggcggacgc
     1921 cggccggcgc gacgcgtcgg cgcggcgatc ccaggcccag ccgacgacgg cgccgacgac
     1981 gaccatcagc gtcgtcgaca tcggcaggta gatgcccagg ccgacggcca gcgggggcag
     2041 ctgcgcgtgg ctgcccggct tgcgccagcg cttgatcgcc tcgtcgagca cgaccagccc
     2101 gacgcccagc gcgacgccct tgccgagcag gttccagtcg aggtcgccgc ccagcacgcc
     2161 cttggcgagc gccgagatca gcgccgcctg cggggcggcc agcgccttgg ccgtggcgcc
     2221 cggcgcgccg gcgaagccgt aggcccgctg cagcagatcg agcaccggcg gaatcacgag
     2281 cgcgcccgcg accgtgccga tggccagcgc cacctgctgc ttccacgggg tcgcgtcgac
     2341 cagctggccg gtcttcaggt cctgcaggtt gtcgttggcg accgtggccg cggcgacgac
     2401 gaccgaggtg acgaacagcg tgaacgcgac cagcgccggg ccggcgctcg ccggcagcat
     2461 cggcttggcc acggcggcga ccagcagcgc cgcgctgatg acgacgatga tgccgacgcc
     2521 ggacagcggg ctgttgctcg agccgatcag cccggccatg tagccgcaca cggcagccac
     2581 gaagaagctc atgagcgcga tgaacgcgat gccgcccgcc acgagcacga cggcggccga
     2641 tccgaggccg gcgcccgagg tcatgtcggc cagcaaccag cccacgggca cgaggcaggc
     2701 cagcgtgacg ccggcgaccc agccgatcgg caggtcctgc tcggtgcgcg gcagcgccgc
     2761 gccgtcgccg gcccggcgca cccgcgaggc ggccatggcg gatgccaggc ccttggccac
     2821 cggcctgacc agcttgacca gcgtccacac ggccgccacg gcgatggcgc cggcgccgat
     2881 gaagcgcacc tgcttggccc acgcgtgcgt cgcgatcgcg gcgacgtcgg cgccggcgtc
     2941 gaacggcagc atggcggtga gctccggcac ggcgccggcc cacgcgatca gcaggccggc
     3001 cagcatggcc aggcccaccc acagcccgac caggtgcccc acggccagca gggccatcga
     3061 caggcccagg tcgacgcccg tggacgcgtt gctgctgcgg ctcaagcgga agtatcccgc
     3121 caggtcggcg gcgaagagct gcgtgccgac gaccagctgg aaggccgccg acgcgacgcc
     3181 gccccatacg atggccagca gcccggcgcg attctcctcg gccgcgccgg cgcccgtgtc
     3241 ggcgcccacc ttgagcacct cggcgcacgc cacgccttcc ggatagggca ggtcggaggt
     3301 cgtcacgagc gcgcgccgca acgggatgga gtaggtcacg cccagcaccc cgccgagcgc
     3361 gcagatcacg acgcaggtga ggtacgggaa gccgttccac cagccgacca tcacgaggcc
     3421 gggcagcacg aagatgatgg acgagatcgc gccggccgcc gaggcgacgg tctgcacgat
     3481 gttgttctcc tggatgctca cgtcgcgcgc acgacgcagc aggcccatgg agatgaccgc
     3541 tgccgggatg gatgtcgcga acgtgagccc ggccttgagg ccgaagtaga cgttggcggc
     3601 cgtgaacagc agtgtgatga ggaagccgag caggacgccg cgcagggtca gctcggatcg
     3661 ggatgggacg gcttcttgca agggatctcc ccgggggatt caaggcgcgg atcatccctc
     3721 aggtgcggcc cggccggcat cgggactaca cgcagacgcc gcgccgaccc tgcggcggcg
     3781 gcaccggcgg ctgcggccgc agctcggagc gccggcgcct cacagcaggt gcagcccgcc
     3841 gtcgcattcg acgatgctgc ccgtcgtgaa gccgttggtg gccatgaaca gcaccgactg
     3901 cgcgacgtcc tcgggccggc cgacgcggcc gaccggcagc gtgcgcgact gctcgtcgaa
     3961 caccgcttcg cgcacggcgg cgggcgcccg gctccaccat gcggtgtcga tgacgcccgg
     4021 ggagaccgcg ttcacgcgcc gtggcgcgag ctcgcgcgcg aggccgccga ccatcgcctc
     4081 gagcgccccg ttgatcgccg ccagccccgt cgtgcccggc cgcgcgatcc gggccgacac
     4141 cgcggtgacg aaggtgatcg acgccgcggt ggcgagcgcc ggcagcgcgg cctggacggt
     4201 ttcgagctgg ggccagaact tcgcctcgaa gccgcgccgc agggcctgga ggtcgagccg
     4261 cgcgaattcg cccgctccct cgccgccgct gagcgtgagc acgaggtggt cgaccggccc
     4321 cgtgtcgcgg aagaacgcgt cgagcgcggc acggtcggtc gcgtccag
//
LOCUS       DASVLN010000714         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_468755, whole genome shotgun sequence.
ACCESSION   DASVLN010000714 DASVLN010000000
VERSION     DASVLN010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468755"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..838
                     /locus_tag="VF453_16830"
     CDS             23..838
                     /locus_tag="VF453_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689379.1"
                     /translation="MRRIDGALRASADPFDRAVADWLDALDALAPPERMARLVEDAVS
                     TADARVYALAFRTCRLPREVPAGGSCDALSARRWAELDRGNALPWVYVYAQARQSGDV
                     SAQQEAMTRMAAADRFEDRAYATAGAVAERVPDEDDALAAGIDLSVRAMGMSAAEMAP
                     ISELTRACRQQAEGDANLAQQCRAVAERMFDRSDTILLRAVGGALYAQATGDPSRREL
                     AHEEQVAIASHWSPGTGLSECGSSRDLLRKFRREARVGELQAVRERLNEAIPP"
     gene            complement(880..>1819)
                     /locus_tag="VF453_16835"
     CDS             complement(880..>1819)
                     /locus_tag="VF453_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7689380.1"
                     /translation="DSRLRHALAQRLGDFGVHASPAQIVTANGATHGLDIVSRALLEP
                     GDAVLVDEPGWSVEFARMTQLGMRLLPVPRGPDGPDLQAMAALAQAHRPRLYVTVSVL
                     HNPTGGSLSLACAHQVLRLAEQHDFRIMEDDTYAHLAPAHAPRLSALDGLRRTIYVSG
                     FAKILTPGWRVGFVAAPPDLVEHLVDTKLLGTLTTSALLEQAVAVCLEQGWLRRHAER
                     VIARLGAARSRSAKLALAAGCRFAAPANGLFGWVDTGVDTERLATDMLDDGWLLAPGT
                     VFHARRRPSTLMRINFATTQDVRFWKAFETARDALA"
BASE COUNT          241 a          646 c          703 g          229 t
ORIGIN      
        1 cgggccgcac tacgccgccg ccgtgcgccg catcgacggc gcgctgcggg ccagcgcgga
       61 tccgttcgac cgcgcggtcg ccgactggct ggacgccctg gatgcgctcg cgccgcccga
      121 gcgcatggca cggctcgtcg aggacgccgt ctccaccgcc gacgcgcggg tctatgcgct
      181 cgccttccgg acctgccggt tgccacggga ggtcccggcc ggcggctcgt gcgacgcatt
      241 gagcgcacgg cgctgggccg agctcgatcg cggcaacgcg ctgccttggg tctacgtcta
      301 cgcgcaggcg cggcagagcg gtgacgtctc tgcgcagcag gaggcgatga cccgcatggc
      361 cgcggccgat cgtttcgagg atcgcgccta cgccaccgcc ggcgccgtgg ccgagcgggt
      421 gcccgacgag gacgacgccc tggccgccgg tatcgatctg tcggtgcggg cgatggggat
      481 gagcgctgcg gagatggcgc cgatctccga gttgacgcgc gcctgccggc agcaggccga
      541 aggcgatgcg aacctcgcgc agcaatgtcg ggcggtcgcc gagcgcatgt tcgatcgctc
      601 ggacacgatc ctgctgcgcg cggtgggcgg cgcgctgtac gcccaggcga ccggcgatcc
      661 gtcgcggcgc gagctcgcgc acgaggaaca ggtggccatc gcgtcgcatt ggtcgcccgg
      721 cacggggctt tccgagtgcg gctcgtcgcg cgacctgctg cgcaagttcc ggcgggaggc
      781 gcgggtgggc gagctgcaag ccgtgcgcga gcgcctgaac gaggcgattc cgccgtgaat
      841 gctcgccggg cgagcacgcg atcgcgaagg cggaggtcct caggccagcg cgtccctcgc
      901 cgtctcgaac gccttccaga accgcacgtc ctgcgtcgtc gcgaagttga tgcgcatcag
      961 cgtcgacgga cggcggcgcg cgtggaagac ggtgccgggc gcgagcagcc agccgtcgtc
     1021 gagcatgtcg gtggccaggc gttcggtgtc gacgccggtg tcgacccagc cgaacaggcc
     1081 gttggccggc gcggcgaagc ggcagccggc cgccagcgcg agcttggcgc tgcgcgagcg
     1141 cgcggcgccc aggcgggcga tgacgcgctc ggcgtggcgg cgcagccagc cctgctcgag
     1201 gcacaccgcg accgcctgct ccagcagcgc cgaggtcgtg agcgtgccca ggagcttggt
     1261 gtcgaccagg tgctcgacga ggtcgggcgg cgcggcgacg aagccgaccc gccagcccgg
     1321 cgtgaggatc ttcgcgaagc ccgacacgta gatggtgcgc cgcagtccgt ccagcgccga
     1381 cagccgcggc gcgtgcgcgg gcgcgaggtg ggcgtaggtg tcgtcctcca tgatgcggaa
     1441 gtcgtgctgc tcggccagcc gcagcacctg gtgcgcgcaa gcaaggctca gcgagccgcc
     1501 ggtcgggttg tgcagcacgg acaccgtcac gtacagccgc gggcgatgcg cctgcgcgag
     1561 cgcggccatc gcctgcaggt ccggcccgtc cggcccgcgc ggcaccggca gcaggcgcat
     1621 gcccagctgc gtcatgcgcg cgaactccac cgaccagccc ggctcgtcca caagcaccgc
     1681 gtcgcccggc tcgagcaagg cgcgcgagac gatgtccagg ccgtgcgtcg cgccgttggc
     1741 cgtgacgatc tgcgccggcg aggcgtggac gccgaagtcg ccgagccgct gggcgagcgc
     1801 gtggcgcagg cgcgagtcg
//
LOCUS       DASVLN010000715         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_251945, whole genome shotgun sequence.
ACCESSION   DASVLN010000715 DASVLN010000000
VERSION     DASVLN010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251945"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /locus_tag="VF453_16840"
     CDS             <1..1808
                     /locus_tag="VF453_16840"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX7689381.1"
                     /translation="PALALILAAFAAAPALAASGAPARAASAASTHEALVSLARTIIE
                     TEARVDPMFATTLGIAGHDGELPTASEAQRAAELDRLRAWGARLEDIRRGAGAQVSLV
                     DADDAKLLRAQLDERLDALLVYQTDRKDYAGPALRVTGAVFTQFLHLPVPGRDGATAA
                     DLAKAWDDIVNRMVAAPAYIEAAQRLVIHPGRLYGTVGARQLAGSPDFLGGALTDAAK
                     EQLAGDPAQLGRFEAARDALLATVAKTRATIDAHVAGWPDNHAMGRSAYDAMLAKEQL
                     LPFDSKALEQMARDELAHGWAEEAWLTALARERHQSFGAETGGGMAPAGQALIPYYRD
                     RIAQLSAFVAQHDVVTVPAWLGQMQVVETPKFLQPVSPGASMDSPRLFAPSTTGYYFI
                     TPPASLDEAAARLDMNEDFDRDRILSTAAHEAMPGHFLQLSIARRHPDYVRKIQYSAA
                     FAEGWAFYGEEMFVRLGLYGDDLDARLFTARWERVRGARVIVDGKLASGEWTLAQAAT
                     FFEQQSGFTKEAAEAAVAGFALRPGYVVSYTVGRLQLETLLAEYLRRMGDKGSLRDFH
                     DRLLSYGCTPFAIVGPELLADLGKSAEQVRAAANY"
BASE COUNT          273 a          767 c          742 g          268 t
ORIGIN      
        1 acccggcgct cgcgctgatc ctcgccgcgt tcgccgcggc gcctgccctc gcggcgtccg
       61 gggcccccgc gcgtgcggct tcggccgcat ccacgcacga agcgctcgtg tcgctcgcgc
      121 gcacgatcat cgagaccgag gcgcgcgtcg acccgatgtt cgcgacgacg ctgggcatcg
      181 cgggccatga cggcgagctg ccgaccgctt ccgaggcaca gcgcgccgcc gagctggatc
      241 ggctgcgcgc ctggggcgcg cggctcgagg acatccgccg tggcgccggc gcgcaggtct
      301 cgctggtcga cgcggacgac gcgaagctgc tgcgtgcgca gctcgacgaa cgcctggacg
      361 cgctgctcgt ctaccagacg gaccgcaagg actacgccgg cccggcgctg cgcgtgaccg
      421 gcgccgtctt cacgcagttc cttcacctgc cggtgccggg ccgcgacggc gcgaccgcgg
      481 ccgacctggc gaaggcctgg gacgacatcg tgaaccgcat ggtcgcggcg ccggcctaca
      541 tcgaggccgc gcagcgcctg gtgatccatc cgggccggct ctacggcacg gtgggcgcgc
      601 gccagctcgc gggctcgccc gacttcctcg gcggcgcgct gaccgatgcc gcgaaggagc
      661 agctcgccgg cgacccggcg cagctcgggc gcttcgaggc tgctcgcgac gccctgctgg
      721 cgaccgtcgc gaagacccgc gccaccatcg acgcgcacgt ggccggctgg cccgacaacc
      781 acgcgatggg ccgctccgcc tacgacgcga tgctcgccaa ggagcagctg ctgcccttcg
      841 acagcaaggc gctcgagcag atggcgcgcg acgagctcgc gcacggctgg gccgaggagg
      901 cctggctcac ggcgctggcg cgcgagcgcc accagtcctt cggtgccgag accggcggcg
      961 gcatggcgcc cgcgggccag gcgctgatcc cgtactaccg cgaccgcatc gcgcagctga
     1021 gcgccttcgt cgcgcagcac gacgtcgtca ccgtgcccgc gtggctgggg cagatgcagg
     1081 tcgtcgagac gccgaagttc ctgcagccgg tgtcaccggg agcctcgatg gactcgccgc
     1141 ggctgttcgc gccgtcgacc accggctact acttcatcac cccgccggcc tccctcgacg
     1201 aggcggccgc gcgcctcgac atgaacgagg acttcgaccg cgaccgcatc ctctcgaccg
     1261 cggcgcacga ggcgatgccg ggccacttcc tgcagctgtc gatcgcgcgc cgccatcccg
     1321 actacgtgcg caagatccag tacagcgcgg ccttcgccga aggctgggcc ttctacggcg
     1381 aggagatgtt cgtgcgcctg ggcctgtacg gcgacgacct cgacgcgcgc ctgttcaccg
     1441 cgcgctggga gcgcgtgcgg ggagcgcgcg tgatcgtcga cggcaagctc gcgagcggcg
     1501 aatggacgct cgcccaggcc gcgaccttct tcgagcagca gtccggcttc acgaaggagg
     1561 ccgccgaggc cgcggtcgcc ggcttcgcgc tgcgccccgg atacgtggtg tcgtataccg
     1621 tcggccgcct gcagctcgag acgctgctgg ccgaatacct gcgccgcatg ggcgacaagg
     1681 gctcgctgcg cgacttccac gaccgcctgc tgtcctatgg ctgcacgccg ttcgcgatcg
     1741 tcggtccgga actgctggcc gacctcggca agagcgcgga gcaggtgagg gcggcggcga
     1801 actactgagg cgcaaggctc ggcgcgaccg gacgacggcc gcgcggcgtc gggccggcgg
     1861 gcgaaagcaa gcccgggctg ccggtcagag cgcgtcgagg tcgcgctgca gccccgtgcg
     1921 gtccggatcg ctcgcctgcg cgagcaccgc ctgcacgaag cgctggcggt cgccgccgga
     1981 ctgctcggcc gcgcgcttga cgatgacgcg ggcgatcggg cccatcttgc gcgtgacgag
     2041 ctggagcgcg
//
LOCUS       DASVLN010000716         1884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_90768, whole genome shotgun sequence.
ACCESSION   DASVLN010000716 DASVLN010000000
VERSION     DASVLN010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90768"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VF453_16845"
     CDS             <1..1287
                     /locus_tag="VF453_16845"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HEX7689382.1"
                     /translation="QAAAWDAWRQSPASAFGFVEAWGAAALAKLARASFPSTRATAGP
                     EREGLQARAALRPRFDLMEQEPQRAAELAHSVLRAMGLLQDFPALVLLVGHGSRTTNN
                     PHAAGLACGACGGQSGELNARVLAALLNDREVRGRLAALGIAIPDATHFLAALHDTVT
                     DDVDCFDVAEIPSSLEPPTRRLLAALRQAGERVRAERAESLGLQRLVDRPAALAQALR
                     RRANDWSELRPEWGLAGNAAFIAAPRTRTRGLDLEGRAFLHDYDWRHDPDGSVLELVM
                     TAPMVVAHWINFQYLASTTAPQRFGSGSKVLHNVVGGRIGVFEGNGGDLRIGLPWQSV
                     HDGTRLRHEPLRLAVFIEAPRAMIDAVLRQHAKVRLLVDNGWLHLLRLDAASAFVERY
                     DRGLWRPLEGEASWADALAAPRLAQGAGHEPRVSRL"
     gene            1499..>1884
                     /locus_tag="VF453_16850"
     CDS             1499..>1884
                     /locus_tag="VF453_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit II"
                     /protein_id="HEX7689383.1"
                     /translation="MRLLACCAAAALLEGCHLTVLDPKGCIAAQEDTLILTATLLMLI
                     VVVPVIFMALYFPWRYRASNTGATYRPKWSYSHRIELAVWAIPCAIILVLGVLTWKTT
                     HSLDPYKPLDSKVKPIEIDVVALDWK"
BASE COUNT          248 a          683 c          675 g          278 t
ORIGIN      
        1 caggcagccg cctgggacgc ctggcgccag tcgccggcct cggccttcgg cttcgtcgag
       61 gcctggggcg ccgcggcact cgccaagctg gcgcgggcca gctttccgtc gacacgcgcc
      121 accgcagggc cggaacgcga aggcctccag gcgcgcgcgg cgctgcgtcc ccggttcgat
      181 ctgatggagc aggagccgca gcgcgcggcg gagctcgcgc actccgtgct gcgggcgatg
      241 ggcctgctac aggacttccc cgcgctggta ctcctcgtgg gccacggcag ccgcacgacc
      301 aacaatccgc atgcggccgg cctggcgtgc ggcgcctgcg gcgggcagag cggcgagctc
      361 aacgcgcgtg tgctcgccgc gctgctgaac gaccgcgagg tgcgcgggcg gctcgcggcg
      421 ctcgggatcg cgatccccga cgccacgcac tttctcgctg cgctccatga cacggtcacc
      481 gacgacgtcg actgcttcga tgtcgccgag ataccgtcct cgctggaacc gccgacccgg
      541 cggctgctcg ccgcgctgcg ccaagcgggc gagcgcgtgc gtgcggagcg cgccgagtcg
      601 ctcgggctgc aacgcctggt cgaccggccc gccgcactgg cccaggccct gcggcggcgc
      661 gcgaacgact ggtccgagct gcggcccgag tggggcctcg cgggcaacgc cgccttcatc
      721 gccgcgccgc gaacgcgcac gcgcggcctc gacctcgaag ggcgcgcctt cctgcacgac
      781 tacgactggc ggcacgaccc cgacggcagc gtgctcgagc tcgtcatgac ggcgccgatg
      841 gtggtcgccc actggatcaa cttccagtac ctggcctcca cgacggcgcc gcagcgcttc
      901 ggcagcggca gcaaggtgct gcacaacgtc gtcggcggtc gcatcggcgt gttcgagggc
      961 aacggcggcg acctgcgcat cggcctgccg tggcagtccg tgcacgacgg cacgcgcctg
     1021 cgccacgagc ccctgcgatt ggccgtcttc atcgaggcgc cgcgcgccat gatcgacgcg
     1081 gtgctgcgcc agcacgcgaa ggtgcgcctg ctcgtggaca acggctggct gcacctgctt
     1141 cgcctggatg ccgcaagcgc cttcgtcgag cgctacgacc gcggtctctg gcggccgttg
     1201 gaaggcgagg cctcgtgggc ggatgcgctt gcagcccccc gcctcgcgca gggagccggc
     1261 cacgagccgc gcgtgagtcg gctttgaatc gcattcgcgt cggcggcgcc gcgggataag
     1321 cggccgccgc gggcggctcg ccgcgttctt gcctacagtg ccgggcatga aacggcgcgg
     1381 cctgccggcg aggcgggtcg cggagatgat catgtgtcca ctcgaaagct ccccgcgcgt
     1441 cgatggcctg gacgtggcga gcggcggacg ccggtcgacg gtccgcggat ggcgggcctt
     1501 gcgtctgctc gcgtgctgcg ccgcggcggc cctgctggag ggctgccacc tcacggtact
     1561 cgatccgaag ggctgcatcg cggcgcagga ggacacgctg atcctcacgg ccaccctgct
     1621 gatgctgatc gtcgtcgtgc ccgtgatctt catggccctt tactttccgt ggaggtaccg
     1681 cgcctcgaac acgggagcga cgtatcggcc gaagtggtcc tactcgcacc gcatcgagct
     1741 ggccgtctgg gccatcccct gcgcgatcat cctggtgctg ggcgtgctga cgtggaagac
     1801 gacgcactcg ctcgacccgt acaagccgct ggattccaag gtcaagccga tcgagatcga
     1861 cgtcgtcgcg ctggactgga agtg
//
LOCUS       DASVLN010000717         8313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_198507, whole genome shotgun sequence.
ACCESSION   DASVLN010000717 DASVLN010000000
VERSION     DASVLN010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198507"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VF453_16855"
     CDS             <1..659
                     /locus_tag="VF453_16855"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7689384.1"
                     /translation="ATGRARTLGDVLAGRTPMLLMLGYDHCPQLCGLALQGALEAAHA
                     SGLPAAAYRLLFVSIDPADTPADAAARERVERDYAHFLAGTAPVPAITLMVGRPPAIA
                     ALAQAVGYRYAATPDAGDARFAHPAALFVVAPDGRISHTFGGIRYDAAELRRAVEDAA
                     AGRVRPGLGDRIALLCAHLDPHLGRWSTSVLAFARIVGLGTVAALGALAWRARRRSAR
                     "
     gene            659..1624
                     /gene="coxB"
                     /locus_tag="VF453_16860"
     CDS             659..1624
                     /gene="coxB"
                     /locus_tag="VF453_16860"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX7689385.1"
                     /translation="MATSVDAELAPGVQLLAPAASTLAHRTDWLMLAMVLLCGTVAAV
                     LCLLVIVFAIRYRRGAHVDRTPPRKLKGLEVAWSAIPLAIFLGIFAWAARDFLTLYAP
                     PRDALPVTVVAKQWMWKVEHRNGRREINELHVPLGEPVVLTLASQDVIHSFFVPAFRV
                     KQDVVPGRWTRLWFKATQPGEYPLFCSEYCGSQHSLMTGRVVVMNPADYARWLASGPA
                     EPSLAQYGFALFRQLGCSGCHDARSTVHAPLLEGVAGRLVHLQDGRTVVADDAYLRDS
                     IVKPGQDVVAGFAPVMPSYAGQVGEEDLAALVAYLQSLSAKDAQP"
     gene            1621..3261
                     /gene="ctaD"
                     /locus_tag="VF453_16865"
     CDS             1621..3261
                     /gene="ctaD"
                     /locus_tag="VF453_16865"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX7689386.1"
                     /translation="MTTDTPRSYLTDGLTIRSWLTSVDHKRIAILYAIAITAFFFVGG
                     AAATIIRLELATPQADVVSHDGYNKVFTAHGVVMVWLFLIPSIPSVLGNFLLPMMLGA
                     KDLAFPRLNLTSWYLYVGGGLVTVWALLLGGVDTGWTFYTPYSTMFSNGYVVLTLVGI
                     FIVGFSSILTGLNFIVTIHVLRPPGMGWFKLPMFAWALYATSLILVLATPVLAITTVL
                     AAAERLFQIGIFDPAYGGDPLLFQHMFWFYSHPAVYIMVLPAMGVVSEIIPCFARNRL
                     FGYPTMAYAILAIASVGFLVWGHHMFVSGQSLAASLAFSLLSFIVAIPSAIKVFNWTA
                     TLYKGAIRFDAPMLYALGFVGLFTIGGLTGLFLASLALDVHLTDTYFVVAHFHYIMVG
                     GAVTAYLGGLHFWWPKATGRMYPEIAARIAAVLIFFGFNMTFFPQFLLGIAGMPRRYH
                     VYPPQFQVLNMFSSAGAGVLAIGYLLPLGYLAWSIRHGPAAGANPFGALGLEWQAPSP
                     PPTHNFDEPPVVPGKVYDYVPDEPEDARTAVRSTEVAS"
     gene            3258..3905
                     /locus_tag="VF453_16870"
     CDS             3258..3905
                     /locus_tag="VF453_16870"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7689387.1"
                     /translation="MSATFAQPAGPRIDTEREPEPGTGALGMWVFLASEVLFFGGLFA
                     AYVYGRTHWPQGWAEASRRTDVVLGTLNTAVLLTSSALIALAVACDAHPRARRHVARL
                     LWATALLGVAFLAIKATEWHDDWIQDLVPGPRFALAAVPGAQEFFVMYFVMTGLHAVH
                     MSAGIGVVASFASGRARGRAWAKAERVEIAALYWHFVDIVWIFLYPLLYLVERHG"
     gene            3898..4326
                     /locus_tag="VF453_16875"
     CDS             3898..4326
                     /locus_tag="VF453_16875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX7689388.1"
                     /translation="MAEAKPRDEREAGRRFARQARAIGLAWIALLALMLASLGSAYVK
                     LGAFNMVAGVAIAATKTLIVAWLFMRLREAGALLRLAALAGLGVYGIQFMLTSVDYAT
                     RVQTPAAVQRPRQLADVPRAVVPDPPASLARPAPPPTYTR"
     gene            complement(4346..4594)
                     /locus_tag="VF453_16880"
     CDS             complement(4346..4594)
                     /locus_tag="VF453_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689389.1"
                     /translation="MSVREGGEDGGTTPGGRPSVPEDDGAGPLPQGGALVPEVLQALR
                     TLREGGHACVPVRGTFPEQFVCVGTADQIRRMFQGGAF"
     gene            4701..4904
                     /locus_tag="VF453_16885"
     CDS             4701..4904
                     /locus_tag="VF453_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689390.1"
                     /translation="MSRKACGKSGALASRGPRHGPCQTEGKPHPAANHSPPERRHIMG
                     KYVLGWLLGVPVTVLVILYLIFH"
     gene            complement(4936..6261)
                     /locus_tag="VF453_16890"
     CDS             complement(4936..6261)
                     /locus_tag="VF453_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX7689391.1"
                     /translation="MTTAAPIRRSAPSDPSHAPADGARERWWHKLGPGLITGAADDDP
                     SGIATYSQGSAQFGFGVGWTVLVTYPLMMGIQLASARIGRITGRGLAENFARLCPRWV
                     VATLVGLLVCANVINLGADLNAMGDAAATTVGGPKAAWALAFGGVSLLLQVFLPYARY
                     VSVLKWLTLSLLAYVGVGFAVHVDWARALRETFVPHLQWGRDYATTVVAILGTTISPY
                     LFFWQAAQEVEEIRRVGPDKPLRIAPEQARRHLRRLRLDTAVGMGFSNAIAFFMIVAT
                     AATLHARGVTHIETTAEAARALEPIAGHLAAWLFSLGVVGTGLLALPVLAGSAAYAVS
                     SLLHVRRGLDQPPAAARAFYAILAAAMLLGAGVGVSGLPPIKALFWSAVVNAVISVPI
                     MVAVMVAASHRRIVGDLALPPAWRALGWIATALMAAATVAMVVLQVAAA"
     gene            complement(6389..6481)
                     /locus_tag="VF453_16895"
     tRNA            complement(6389..6481)
                     /locus_tag="VF453_16895"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6445..6447),aa:Ser,seq:gct)
     gene            complement(6518..7795)
                     /locus_tag="VF453_16900"
     CDS             complement(6518..7795)
                     /locus_tag="VF453_16900"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7689392.1"
                     /translation="MALIVHKYGGTSMGSTERIRNVAKRVAKWARAGHQMVVVPSAMS
                     GETNRLLGLAKELFPGTDTSASMRELDMIASTGEQVSVGLLSLALQHEGLEAVSYTGW
                     QVAVKTDSSYTKARIESIDDERVRADLAAGRVVVITGFQGIDEAGHITTLGRGGSDTS
                     AVAIAAAMKADECLIYTDVDGVYTTDPRVVPEARRLSTISFEEMLEMASLGSKVLQIR
                     SVEFAGKYRVPLRVLSSFTPWDIDINEEARSGTLITFEEDVNMEQAVVSGIAFNRDEA
                     KVTVMGVPDKPGIAYQILGDVAEANIDVDVIIQNVSHDGKTDFSFTVHRNDYARTLEL
                     LKTKVAPATGASQVIGDPKICKVSIVGIGMRSHVGVASKMFRTLSEEGINIMMITTSE
                     IKTSVVIDEKYMELAVRALHKAFDLEDPAKTAG"
     gene            complement(7992..>8313)
                     /locus_tag="VF453_16905"
     CDS             complement(7992..>8313)
                     /locus_tag="VF453_16905"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TilS substrate C-terminal domain-containing
                     protein"
                     /protein_id="HEX7689393.1"
                     /translation="PGWDGLLEVMPVAAGGIDPARLREVFPRARSGGERFRRRPDAPP
                     RSLKKQYQAAGVPEEGRAGPLLYDRDGALLFVPGLGIDAACRAPEGARQVALHWRSGA
                     LAED"
BASE COUNT         1125 a         3004 c         2850 g         1334 t
ORIGIN      
        1 acgcgacggg ccgcgcacgc acgctcggcg acgtgctcgc gggacgcacc ccgatgctgc
       61 tgatgctggg ctacgaccac tgcccgcagc tgtgcggcct cgcgctgcag ggggcgctcg
      121 aggccgcgca cgcgagcggc ctgcccgccg cggcctatcg gctgctgttc gtgagcatcg
      181 atccggccga cacccccgcc gacgcggctg cacgcgaacg cgtcgagcgc gactatgcgc
      241 acttcctggc cggcaccgcg cccgtgcccg ccatcacgct gatggtcggc cggccgcccg
      301 cgatcgcggc gctcgcacag gcggtggggt atcgctacgc cgccacgccc gacgccggag
      361 acgcgcgctt cgcgcacccg gcggcgctgt tcgtcgtcgc tcccgacggg cgcatctcgc
      421 atacctttgg cggtatccgg tacgacgccg ccgagctgcg ccgcgcggtc gaggacgccg
      481 ccgccggccg cgtcaggccg ggcctgggcg accgcatcgc cctgctctgc gcgcacctcg
      541 atccgcacct gggccgctgg agcacctcgg tgctggcgtt cgcgcgcatc gtcggcctcg
      601 gcaccgtcgc ggcgctgggg gcccttgcct ggcgggcgcg ccgccggagt gcccgctgat
      661 ggccacgagc gtcgacgccg agctggcccc gggcgtgcag ctgctcgcgc cggcggcctc
      721 gaccctcgcc catcggacag actggctgat gctcgccatg gtgctgctgt gcggcacggt
      781 cgcggcggtg ctgtgcctgc tcgtcatcgt gttcgcgatc cgctaccggc gcggcgcgca
      841 cgtcgaccgc acgccgccgc gcaagctcaa gggcctggag gtcgcatggt ccgcgatccc
      901 gctggcgatc ttcctgggca tcttcgcgtg ggccgcgcgc gacttcctga cgctgtatgc
      961 gccaccgcgc gacgcgctgc cggtcaccgt cgtcgccaag cagtggatgt ggaaggtcga
     1021 gcaccgcaac ggccggcgcg agatcaacga gctgcacgtg ccgctgggcg agccggtggt
     1081 gctcacgctc gcgtcgcagg acgtcatcca cagcttcttc gtgccggcgt tccgcgtgaa
     1141 gcaggacgtc gtgccgggcc gctggacgcg gctgtggttc aaggcgacgc agccgggcga
     1201 gtatccgctg ttctgttccg aatactgcgg cagccagcat tccctgatga cggggcgcgt
     1261 cgtcgtgatg aacccggccg actacgcgcg ctggctcgcg tccggcccgg ccgagccttc
     1321 gctcgcgcag tacggcttcg cgctgttccg ccagctcggc tgctcgggct gccacgacgc
     1381 ccgctccacg gtgcatgcgc cgctgctgga gggcgtcgcc ggccgtctcg tgcacctgca
     1441 ggacggccgc acggtcgtgg ccgacgacgc ctacctgcgc gactcgatcg tgaagcccgg
     1501 acaggacgtc gtcgccggct tcgcgccggt catgccctcg tacgcgggcc aggtgggcga
     1561 ggaagacctc gcggcgctgg tggcctacct gcagtcgctc tcggcgaagg atgcgcagcc
     1621 atgacgaccg ataccccccg ctcgtacctc accgacggcc tgacgatccg ttcgtggctg
     1681 acctcggtcg accacaagcg catcgcgatc ctctacgcca tcgcgatcac cgcctttttc
     1741 ttcgtgggcg gtgcggcggc gacgatcatc cgcctcgagc tcgcgacgcc gcaggccgac
     1801 gtcgtctccc acgacggcta caacaaggtg ttcacggccc acggcgtcgt gatggtgtgg
     1861 ctgttcctca ttccctcgat cccgtcggtg ctcggcaact tcctgctgcc gatgatgctg
     1921 ggcgcgaagg atctcgcgtt cccgcggctg aacctgacgt cgtggtacct gtacgtcggc
     1981 ggcgggctcg tgacggtgtg ggcgctgctg ctgggcggcg tcgacacggg ctggacgttc
     2041 tacacgccgt actccacgat gttctccaac ggctacgtgg tgctgacgct ggtgggcatc
     2101 ttcatcgtcg gcttctcgtc catcctcacc gggctgaact tcatcgtcac gatccatgtg
     2161 ctgcgcccgc cgggcatggg ctggttcaag ctgccgatgt tcgcgtgggc gctgtacgcg
     2221 acgagcctga tcctcgtgct cgccacgccc gtgctcgcga tcacgacggt gctcgcggcg
     2281 gccgagcggc tgttccagat cggcatcttc gatcccgcgt acggcggcga tcccctgctg
     2341 ttccagcaca tgttctggtt ctactcgcac ccggcggtct acatcatggt gctgccggcg
     2401 atgggggtgg tcagcgagat catcccctgc ttcgcccgca accgcctgtt cggatacccg
     2461 acgatggcct acgcgatcct ggcgatcgcg agcgtcggct tcctcgtctg gggccaccac
     2521 atgttcgtct cggggcaatc gctcgcggcc agcctggcgt tctcgctgct gagcttcatc
     2581 gtcgcgatcc cgtcggcgat caaggtgttc aactggacgg ccacgctcta caagggcgcc
     2641 atccgcttcg acgcgccgat gctgtatgcg ctcgggttcg tgggcctgtt cacgatcggc
     2701 ggcctcacgg ggctgttcct cgcttcgctc gcgctggacg tgcacctcac cgacacctac
     2761 ttcgtggtcg cgcacttcca ctacatcatg gtcggcggcg ccgtgactgc ctatctcggt
     2821 ggattgcact tctggtggcc gaaggcgacc ggccgcatgt atccggagat cgccgcgcgc
     2881 atcgcggcgg tgctgatctt cttcgggttc aacatgacgt tcttccccca gttcctgctc
     2941 ggcatcgccg gaatgcctcg ccgctaccac gtataccccc cgcagttcca ggtgctcaac
     3001 atgttctcgt cggcgggcgc gggcgtgctc gcgatcggct acctgctgcc cctgggctat
     3061 ctcgcgtggt cgatacggca cggacccgcc gcgggggcca acccgttcgg cgccctcggc
     3121 ctggagtggc aggcgccctc cccgccgccg acccacaact tcgacgagcc gcccgtggtg
     3181 ccgggcaagg tctacgacta cgtgcccgac gagcccgaag acgctcgcac cgcagtgcgc
     3241 agcacggagg tcgcctcgtg agcgccacct tcgcgcagcc cgcagggccc cgcatcgaca
     3301 cggagcgcga gcccgagccc ggcaccggcg cgctgggcat gtgggtgttc ctcgcgagcg
     3361 aggtgctgtt cttcggaggc ctgttcgcgg cctacgtcta cggccgcacg cactggccgc
     3421 agggctgggc agaggcatcc aggcgcaccg acgtcgtgct gggcacgctc aacacggcgg
     3481 tgctgctgac cagcagcgcg ctcatcgcac tcgcggtcgc gtgcgacgcg cacccgcgcg
     3541 cgcgccgtca cgtcgcgcgg ctgctgtggg cgacggcgct cctgggcgtc gcgttcctcg
     3601 cgatcaaggc caccgagtgg cacgacgact ggatccagga cctggtgccc ggcccgcgct
     3661 tcgcgctcgc cgccgtgccg ggtgcgcagg aattcttcgt gatgtatttc gtgatgaccg
     3721 gcctgcatgc cgtgcacatg agcgccggca tcggcgtggt cgcgagcttc gcgtccgggc
     3781 gggcgcgcgg gcgcgcatgg gcgaaggccg agcgcgtcga gatcgccgcc ctgtactggc
     3841 atttcgtcga catcgtctgg atcttcctgt acccgctgct gtacctggtg gagcgccatg
     3901 gctgaggcga agccgcgcga cgagcgcgag gccgggcgcc ggttcgcgcg ccaggcgcgt
     3961 gcgatcgggc tcgcgtggat cgcgctgctc gcgctgatgc tggcgagcct gggcagcgcc
     4021 tacgtgaagc tgggggcgtt caacatggtc gcgggcgtgg ccatcgccgc gaccaagacg
     4081 ttgatcgtcg cgtggctgtt catgcggctg cgcgaggcgg gcgcgctgct gcggctggcc
     4141 gctctggcgg gcttgggcgt ctacgggatc cagttcatgc tgaccagcgt cgactacgcg
     4201 acccgcgtgc agacgcccgc ggcagtgcag cgcccgcgcc agctcgcgga cgtgccgcgc
     4261 gccgtcgtgc cggatccgcc agcctcgctt gcgcgtccgg cgccgccccc gacctacacg
     4321 cgctgacgcg ggcgcgtggg cggcctcaga aggcgccgcc ctggaacatc cgccggatct
     4381 ggtcggccgt gccgacgcag acgaactgtt cgggaaacgt gccgcgcacg ggcacgcagg
     4441 cgtggccccc ctcgcgcagc gtgcgcagcg cctgcagtac ttccggaacc agcgcaccac
     4501 cctgcggcaa cggcccggcg ccgtcgtcct cgggaacgga cggccggccg cccggcgtcg
     4561 tgcccccatc ctcgccgcct tcgcgcacgc tcatcgcaca tccggcgcca tgcgccgccc
     4621 gctgtccgcg cgccgcggtc ccgccccggc gcgaccccgc aacgcgcccg gttccatctg
     4681 cctcgacatg acgcctccgt gtgtcccgca aggcctgcgg caagtccggt gccttggcgt
     4741 cgcggggccc gcggcatggg ccttgccaga ccgaggggaa gccgcatccc gcggcgaatc
     4801 actccccacc cgaaaggaga cacatcatgg gcaagtacgt actgggctgg ctgctgggcg
     4861 tgccggtgac ggtgctggtg atcctctacc tgatcttcca ctgacagcga cggccgcggc
     4921 ccgggcgccc acgcctcagg ccgcggccac ctgcagcacg accatcgcca ccgtcgccgc
     4981 ggccatgagc gcggtcgcga tccagccgag cgcgcgccac gccggcggca gcgccaggtc
     5041 gccgacgatg cggcggtgcg acgccgccac catcacggcg accatgatgg gtaccgagat
     5101 caccgcattg acgaccgccg accagaacag cgccttgatc ggcggcagcc ccgacacgcc
     5161 gaccccggcc cccaacagca ttgccgccgc gaggatcgcg tagaacgcgc gcgcggccgc
     5221 cggcggctgg tcgaggccgc gccgcacgtg caggaggctg gagaccgcat aggccgcgga
     5281 gcccgccagc accggcagcg cgagcaggcc cgtgcccacc acgccgagcg agaacagcca
     5341 cgccgccagg tggccggcga tcggctcgag cgcgcgcgcg gcctcggccg tcgtctcgat
     5401 gtgcgtcacg ccgcgagcgt gcagggtcgc ggcggtcgcg acgatcatga agaacgcgat
     5461 cgcgttggag aagcccatgc ccaccgccgt atccagccgc agccggcgca gatggcggcg
     5521 cgcctgttca ggagcgatgc gcagcggctt gtcggggccg acgcggcgga tctcctcgac
     5581 ctcctgcgcc gcctgccaga agaacaggta cgggctgatc gtggtgccca gtatcgcgac
     5641 cacggtggtc gcgtagtcgc ggccccactg caggtgcggc acgaaggtct cgcgcagcgc
     5701 ccgcgcccag tcgacatgga ctgcgaagcc gacgccgacg taggcgagca gcgacagcgt
     5761 gagccacttc agcaccgaca cgtagcgtgc gtagggcagg aacacctgca gcagcagcga
     5821 cacgccgccg aaggccagcg cccacgcggc cttcggcccg ccgacggtgg tcgcggcggc
     5881 gtcgcccatc gcgttcaggt cggcgccgag gttgatcacg ttggcgcaga ccagcaggcc
     5941 caccagggtg gcgacgaccc agcgcggaca caggcgcgcg aagttctcgg ccagcccgcg
     6001 cccggtgatg cggccgatgc gtgcgctcgc gagctggatg cccatcatca gcggataggt
     6061 cacgagcacc gtccagccca cgccgaagcc gaactgcgcg ctgccctgcg agtaggtggc
     6121 gatgccgctc gggtcgtcgt ccgccgcgcc cgtgatcagg ccggggccca gcttgtgcca
     6181 ccagcgctcg cgcgcaccgt ccgccggggc gtgggaggga tcggacggag cggatcggcg
     6241 gatcggggcg gcggtggtca aggggactcc ggtgcggcgg ccccactcga cagggccgtc
     6301 ggccgaagag caagtcgcgc gcccggttgt cgcgtcgccg gcgacgaaaa aaaaatgcgc
     6361 ccgtcggggc gcattcttca aggtcggttg gcggagacga agggattcga acccttgatg
     6421 cagctcatca ccgcatactc ccttagcagg ggagcacctt cggccactcg gtcacgtctc
     6481 caacctaagc tggcattcta acctggaatt ttgtcgctca gccagcggtc ttcgcaggat
     6541 cctcgaggtc gaacgccttg tgcagggcgc gcaccgcgag ttccatgtac ttctcgtcga
     6601 tcacgacgct cgtcttgatc tcgctggtgg tgatcatcat gatgttgatc ccctcctcgc
     6661 tgagcgtgcg gaacatcttg ctcgcgacgc ccacgtggct gcgcatgccg atgcccacga
     6721 tcgagacctt gcagatcttc ggatcgccga tgacctggct cgcgccggtg gcgggcgcga
     6781 ccttggtctt caggagctcc agcgtgcgcg cgtagtcgtt gcggtgcacg gtgaaggaga
     6841 agtcggtctt gccgtcgtgc gagacgttct ggatgatgac gtcgacgtcg atattggcct
     6901 cggccacgtc gcccaggatc tggtaggcga tgcccggctt gtccggcacg cccatcaccg
     6961 tgaccttggc ctcgtcgcgg ttgaaggcga tgcccgaaac gactgcttgt tccatgttga
     7021 cgtcttcctc gaaagtgatc agcgtgcccg agcgggcctc ttcgttgatg tcgatgtccc
     7081 agggcgtgaa gctggagagc acgcgcagcg gcacgcggta cttgcccgcg aattccaccg
     7141 agcggatctg cagcaccttg gagccgaggc tggccatctc gagcatctcc tcgaacgaga
     7201 tggtggacag ccggcgcgcc tcgggcacga cccgcgggtc ggtcgtgtag acgccgtcga
     7261 cgtccgtgta gatcaggcac tcgtcggcct tcatcgccgc ggcgatcgcg acggccgaag
     7321 tgtccgagcc gccgcggccc agcgtcgtga tatggccggc ctcgtcgatg ccctggaagc
     7381 cggtgatgac cacgacccgg ccggcggcga ggtcggcgcg cacgcgctcg tcgtcgatgc
     7441 tctcgatgcg cgccttggtg tacgacgagt ccgtcttcac cgcgacctgc cagccggtgt
     7501 agctcaccgc ctcgagtcct tcgtgctgca gcgcgagcga cagcaggccg accgagacct
     7561 gctcgcccgt ggacgcgatc atgtcgagct cgcgcatcga cgccgaggtg tcggtgcccg
     7621 ggaacagctc cttcgcgagc cccagcaggc ggttggtctc gccgctcatg gccgacggga
     7681 cgacgaccat ctggtgtccg gcgcgcgccc atttcgcgac gcgtttggcg acgttgcgaa
     7741 tccgctccgt cgaacccatc gaggtgccgc cgtacttgtg aactatcaat gccatggtct
     7801 ggactgcctg gttgacactg cgggccttgc cgatgcgcgc gcccgcgcga accgcgtagt
     7861 ctagcgtgcg taccggggtt tcccccagga cgcccggttc gcgcatggtc gagcggtgct
     7921 tctcgttgcg cgaagccgac ttgtctagac aggtcgagcc ttcgcctcgc gcgcaggcga
     7981 tccgaccccg atcaatcctc cgcaagcgcg cccgaacgcc agtgcagggc gacctgtcgc
     8041 gcgccttccg gggcccggca tgcggcatcg atccccagcc cgggcacgaa cagcagcgcg
     8101 ccgtcgcggt catacagcag cggtccggcg cgcccttcct cggggacgcc ggcggcctgg
     8161 tactgcttct tcagcgagcg cggcggcgca tccgggcggc gccggaagcg ctcgccgccc
     8221 gatcgcgcgc gcgggaagac ttcccgcagg cgggccgggt cgatgccgcc ggcggccacg
     8281 ggcatgacct cgagcaggcc gtcccagccg ggc
//
LOCUS       DASVLN010000718         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_359319, whole genome shotgun sequence.
ACCESSION   DASVLN010000718 DASVLN010000000
VERSION     DASVLN010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359319"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..388)
                     /locus_tag="VF453_16910"
     CDS             complement(26..388)
                     /locus_tag="VF453_16910"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX7689394.1"
                     /translation="MSTSLMPLLAFVVVIALIPVVLWTMKRAGVGNAQSGNVLRHVAQ
                     IGVGSTQRVVVVEIAVGPQRHWLVLGVTGEQVTQIASYPAPDTPQSPDAPAHAMAVNQ
                     LINRWRGGAGAQGGPRHD"
     gene            complement(492..929)
                     /gene="fliN"
                     /locus_tag="VF453_16915"
     CDS             complement(492..929)
                     /gene="fliN"
                     /locus_tag="VF453_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX7689395.1"
                     /translation="MATEGMSDDDAMAAEWAAALAESKTSEAGGMSGAVQTETVAPAS
                     FTNFAPTDGMSGAGNDLNMILDIPVQLTVELGRTRIPIKHILQLAQGSVVELDALAGE
                     PMDVLVNGFLIAQGEVVVVNDKFGIRLTDIVTPSERMRRLSRT"
     gene            complement(919..1923)
                     /gene="fliM"
                     /locus_tag="VF453_16920"
     CDS             complement(919..1923)
                     /gene="fliM"
                     /locus_tag="VF453_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEX7689396.1"
                     /translation="MNQQILSQDEVDALLQGITGESQKLEQEETSEGAIRNYDLANQE
                     RIVRGRMPTMEIIGERFARNIRIGLFNFIRKSPEVSINGIKVQKYSAFLREIVVPTNF
                     NIVSVRPLRGSGLIVCDPSLVFAVIDALFGGVGKFHTRIEGRDFSPTEQRVIQRLVDC
                     IITEYRNAWQGIYPLDLDYQRSEMQPQFANIATPSEIVVASSFTLEVGETSGSVHFCI
                     PYSTLEPIRDVLYSTIQGDSVETDRRWVNLMKAQIQSAEVDLVAELGYAPATVEQLLS
                     FKPGDFIELDLDPLIQAKVDGVPVFDCHYGTSAGKYAVKIDKLLTSSNLSWMGDGNGN
                     "
     gene            complement(1955..2566)
                     /locus_tag="VF453_16925"
     CDS             complement(1955..2566)
                     /locus_tag="VF453_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX7689397.1"
                     /translation="MSAAAAAADAPAGKKPGGKKKLVVMIAVAALVLGGGGVGGLMFL
                     QHQKAAAAAAAADGDDDAPVAKAATKKKSDKPPVFVPMDPFVVNLADHDADRYAQIGI
                     TLEVPDEKVGEDIKTYLPAIRNNILLLLAHKSTADLAGGDGKEQLAREIRREALKAMG
                     EDVPDEDDDAAASAPARKKKKHKKAEAEDSPIRSVQFSSFIIQ"
BASE COUNT          394 a          910 c          961 g          493 t
ORIGIN      
        1 ggcgacggca cggagcgcgc ggcgctcagt catggcgcgg ccccccttgc gcgccggcgc
       61 cgccgcgcca gcggttgatc agctggttga ccgccatcgc gtgcgcgggc gcgtcgggcg
      121 actgcggcgt gtcgggcgcg ggatagctgg cgatctgcgt gacctgctcg ccggtcacgc
      181 ccagcaccag ccagtggcgc tgaggcccca cggcgatctc cacgaccacc acgcgctgcg
      241 tcgagccgac gccgatctgc gcgacatggc gcagcacgtt gccgctctgg gcgttgccga
      301 cgccggcgcg cttcatggtc cacaggacga cggggatcag ggcgatcacc accacgaagg
      361 ccagcagggg catcagggat gtgctcatca gggctttctc tttgtcgaca cgcgcatcga
      421 ggcgcggaag cgtgggccga cgatacgccg cccggcgccg ggcctgcaag agcccgacga
      481 tagccgcatt ttcaggtgcg gctgaggcgg cgcatgcgct cgctgggcgt cacgatgtcg
      541 gtcaggcgga tgccgaactt gtcgttgacg accacgacct cgccctgcgc gatcaggaag
      601 ccgttgacca gcacgtccat cggctcgccg gccagcgcgt cgagctcgac caccgagccc
      661 tgcgccagct gcaggatgtg cttgatcggg atgcgggtgc ggcccagttc cacggtcagc
      721 tggacgggga tgtccaggat catgttcagg tcgttgcccg cgcccgacat gccgtcggtc
      781 ggcgcgaaat tcgtgaagct ggcgggcgcg accgtctcgg tctgcaccgc gccgctcatg
      841 ccgccggcct cgctggtctt ggactccgcc agcgcggccg cccattcggc ggccatcgcg
      901 tcgtcgtcgc tcatgccttc agttgccatt gccgtctccc atccagctca ggttcgagct
      961 ggtcaacagt ttgtcgatct tgaccgcgta cttgcccgcc gacgtgccgt agtggcagtc
     1021 gaacaccggc acgccgtcca ccttggcctg gatcagcggg tcgaggtcga gctcgatgaa
     1081 gtcgcccggc ttgaaggaca gcagctgctc caccgtcgcc ggcgcgtagc cgagctcggc
     1141 gaccaggtcg acctcggccg actggatctg cgccttcatc aggttgaccc agcggcggtc
     1201 ggtctcgacc gagtcgccct ggatcgtcga atacagcacg tcccggatcg gctcgagcgt
     1261 cgagtacggg atgcagaagt gcaccgagcc cgaggtctcg cccacttcca gcgtgaagct
     1321 ggaggcgacg acgatctcgc tgggcgtggc gatgttggcg aactggggct gcatctccga
     1381 gcgctggtag tccaggtcga gcgggtagat gccctgccac gcgttgcggt actcggtgat
     1441 gatgcagtcg accaggcgct ggatcacgcg ctgctcggtg ggcgagaagt cgcggccctc
     1501 gatgcgcgta tggaacttgc cgacgccgcc gaacaaggca tcgatgaccg cgaacaccag
     1561 gctcgggtcg cacacgatca gcccggagcc gcgcagcggg cgcaccgaga cgatgttgaa
     1621 gttggtcggc accacgatct cgcgcaggaa ggccgagtac ttctgcacct tgatgccgtt
     1681 gatcgagact tccgggctct tgcggatgaa gttgaacagg ccgatgcgga tgttgcgggc
     1741 gaagcgctcg ccgatgatct ccatcgtcgg catgcggccg cggacgatac gctcctggtt
     1801 ggccaggtcg tagttgcgga tcgcgccttc ggaagtctct tcctgctcga gcttctggct
     1861 ctcgccggtg atgccctgca gcagggcatc gacttcgtcc tgggaaagga tctgctggtt
     1921 catggcgtgc gtgcctgaag cctcgtgccg tcgcttactg gatgatgaag ctggagaact
     1981 gcaccgagcg gatcggcgag tcctcggcct cggccttctt gtgcttcttc ttcttgcggg
     2041 ccggggcgga cgcggcggcg tcgtcgtcct cgtcgggcac gtcctcgccc atggccttca
     2101 gcgcctcgcg gcggatctcg cgcgcgagct gctccttgcc gtcgccgccg gccaggtccg
     2161 cggtggactt gtgggcgagc agcagcagga tgttgttgcg gatggcgggc aggtaggtct
     2221 tgatgtcctc gccgaccttc tcgtcgggga cttcgagcgt gatgccgatc tgcgcgtagc
     2281 ggtcggcgtc gtggtcggcc aggttgacga cgaacggatc catcggcacg aagacgggcg
     2341 gtttgtcgct cttcttcttg gtcgcggcct tggcgaccgg cgcatcgtcg tcgccgtcgg
     2401 cggcagcggc ggcggcggcc gccttctggt gctgcaggaa catcagcccg cccacgcccc
     2461 cgccgccgag gacgagcgcg gcgacggcga tcatgacgac cagcttcttc tttcccccgg
     2521 gcttcttgcc ggcgggggca tctgcagcgg cggcggcagc ggacatggac tcggactcct
     2581 tggacgtttg atcggcacgc gcgcggacgg cgagcaccat tgacgtcgat tattcggatc
     2641 cgcgccgcct cgaatgagtg gaaaagggcc ggttttcagg acctcaggcg tacatgtcca
     2701 gcaggccgcg ccgctgcgcc gcctggcgcg ccgcgctggc ggccgcgaca tcttcgag
//
LOCUS       DASVLN010000719         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_252221, whole genome shotgun sequence.
ACCESSION   DASVLN010000719 DASVLN010000000
VERSION     DASVLN010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252221"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VF453_16930"
     CDS             complement(<1..832)
                     /locus_tag="VF453_16930"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7689398.1"
                     /translation="MRLRYIEIFHAVMQAGTVKGAAELLHITQPAASRLLQQAEANLG
                     TPLFQRDRGRLLPTQEAIILRPEIEQLHARLDEVRRIATTLADGEDVSLKVLSEPALA
                     VEFLPAACARLMRRKPRLKLTVHTLHTRQLCESLALREAHIGFAFAPSSHPAIASSPV
                     AEAELVCVGLDLPDGPMSLEQAVSGPVVDLAPDDPLREILHGAVDALDLPMNSSAFVH
                     SYHAAIEFAHQGLGRAIVDRFSARYASRYPRLRVRPIVPAIPMTVYCLRPTGLPSNAS
                     A"
     gene            complement(1106..>2549)
                     /locus_tag="VF453_16935"
     CDS             complement(1106..>2549)
                     /locus_tag="VF453_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LWXIA domain-containing protein"
                     /protein_id="HEX7689399.1"
                     /translation="ARDLANRQIDDATQARAKLNAAQAALRDAKADATRATSDAASRK
                     AAATQAATASMKDAMASLRDRTSGDTAGRRGISPASQARISQVLDQLEADPAGKGLPA
                     QLRAATTDVVLDTNSKLGKGWKHYKQFTWYVSAARAPARWGMDALFDRSNWSFGHSWA
                     EYFSNGLGGNSGNASNVTGTFANVFNMFAENAGRFRNNVDKAFWGRIGGKADALDTVS
                     FAAWGGQNVVDLTWAGTTHPVATGIQLAGNVVAGLGLIRDSKNWQKIPFAKNPHPWVN
                     PLLVGLGVGALLVPYYATQPMFNAPASSPPPNPNPTPTTSPTSTASATPTTAATPSNG
                     TTPTAGATPTAGSTPTTSASPTTSSPPGTTTKGQPAGPGSPPTTGTPPGTGTGTRKPT
                     SPAQIVVTAGDPRRDTLWGIAQANEGTLLTPAQVEAAHGENGKVVAALGELIQINPKH
                     GFVLDLDGSQDDVDIIQPGWLINVLNPALN"
BASE COUNT          314 a          822 c         1001 g          412 t
ORIGIN      
        1 tcgccgaggc gttggacggc aggccggtcg gccgcaggca gtacaccgtc atcgggatcg
       61 cgggcacgat gggccgcact cgcagccggg ggtagcggct cgcgtagcgg gcgctgaagc
      121 ggtcgacgat ggcgcggccc agtccctggt gggcgaactc gatcgccgcg tggtagctgt
      181 ggacgaatgc gctcgagttc atcggcaggt ccagcgcgtc gaccgcgccg tgcaggatct
      241 cgcgcagcgg gtcgtcgggt gcgaggtcga cgacgggtcc cgagacggcc tgctcgagcg
      301 acatcggacc gtcgggcagg tccagtccga cgcacaccag ctcggcctcg gcgaccggcg
      361 agctggcgat cgcgggatgc gacgacggcg cgaaggcgaa gccgatgtgg gcctcgcgca
      421 gcgcgaggct ctcgcacagc tggcgagtgt gcagggtgtg caccgtcagc ttcagccggg
      481 gcttgcgccg catcaggcgg gcgcaggcgg cgggcaggaa ctcgaccgcc agcgcgggtt
      541 cggacagcac cttgagcgac acgtcctcgc cgtcggccag cgtcgtggcg atgcggcgca
      601 cctcgtcgag gcgggcgtgg agctgctcga tctcgggccg caggatgatc gcctcctgcg
      661 tcggcagcag ccggccgcgg tcgcgctgga acagcggcgt gccgaggttg gcctcggcct
      721 gttgcagcag gcgggaggcg gccggctgcg tgatgtggag cagttcggcc gctcccttca
      781 ccgtgccggc ctgcatcacg gcgtggaaga tctcgatgta gcgcagccgc atgtcgtttc
      841 cggggcgctc cggacggcgg gggaatcgtc gggcgcggac ggcactcctg tctcgagcgt
      901 cgagaagcca acgcttcatg ttgcagatcg gcgatcggtg gacgagccgg tcccgccgca
      961 ggcggcggca gggccttgtt tcaccgaatc gacatgtctg caagatatcg acgatcgagg
     1021 cggaagtgaa ctacgagaat ccgagcggcg cgcccgctcc cgcggacggc cgcccggatt
     1081 cccgctgcat gcggtcgggg cggattcagt tcagggccgg gttgagcacg ttgatcagcc
     1141 agcccggctg gatgatgtcg acgtcgtcct gcgagccgtc caggtccagc acgaagccgt
     1201 gcttggggtt gatctggatc agctcgccca gcgcggcgac gaccttgccg ttctcgccgt
     1261 gcgccgcctc gacctgggcg ggcgtgagca gggtgccctc gttggcctgc gcgatgcccc
     1321 acagggtgtc gcggcgcggg tcgccggccg tgacgacgat ctgcgcgggc gacgtcggct
     1381 tgcgggtgcc ggtaccggta ccgggaggcg tgccggtcgt gggcggcgag cccgggcccg
     1441 cgggctggcc cttggtcgtg gtgccgggcg gactcgacgt ggtcggcgag gcgctcgtgg
     1501 tcggggtgga tcctgcggtc ggcgtcgccc cggcggtcgg tgtggtgccg ttcgagggcg
     1561 tcgcggccgt cgtgggcgtg gcgctggccg tcgaggtggg gctggtcgtc ggcgtgggat
     1621 tggggttcgg cggcgggctg gatgccggcg cgttgaacat gggctgggtg gcgtagtagg
     1681 gcacgaggag cgcaccgacg ccgaggccga ccagcagcgg gttcacccac ggatgcgggt
     1741 tcttcgcgaa ggggatcttc tgccaattct tcgaatcgcg gatgaggccg agtcccgcca
     1801 cgacgttgcc ggcgagctgg atcccggtcg cgaccggatg ggtcgtgccc gcccaggtga
     1861 ggtcgacgac gttctgtccg ccccacgcgg cgaacgacac ggtgtccagc gcgtccgctt
     1921 tcccgccgat ccggccccag aacgccttgt cgacgttgtt gcggaaacgg cccgcgttct
     1981 cggcgaacat gttgaacacg ttggcgaacg tgcccgtgac gttggaggcg ttgccggagt
     2041 tgccgcccag tccgttcgag aagtactcgg cccacgaatg cccgaagctc cagttgctgc
     2101 ggtcgaacag ggcgtccatg ccccagcgcg cgggcgcgcg ggcggccgag acgtaccagg
     2161 tgaactgctt gtagtgcttc cagcccttgc cgagcttgct gttcgtgtcg aggacgacgt
     2221 cggtcgtcgc ggcgcgcagc tgcgcgggca ggcccttgcc ggccggatcg gcttcgagct
     2281 ggtcgagcac ctgggagatc ctggcctgcg acgccgggct gatcccgcgc cgaccggcgg
     2341 tgtcgccgct cgtgcggtcg cgcagggagg ccatcgcgtc cttcatgctg gcggtggcgg
     2401 cctgcgtcgc ggccgccttg cgcgaggcgg cgtcgctggt ggcccgcgtc gcgtccgcct
     2461 tcgcgtcccg caaggcggcc tgcgccgcgt tgagcttggc gcgcgcctgg gtggcgtcgt
     2521 cgatctgccg gttcgccagg tcgcgtgcg
//
LOCUS       DASVLN010000720        14572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_91012, whole genome shotgun sequence.
ACCESSION   DASVLN010000720 DASVLN010000000
VERSION     DASVLN010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14572
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91012"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VF453_16940"
     CDS             <1..141
                     /locus_tag="VF453_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7689400.1"
                     /translation="EMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKI
                     IE"
     gene            157..232
                     /locus_tag="VF453_16945"
     tRNA            157..232
                     /locus_tag="VF453_16945"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:190..192,aa:Trp,seq:cca)
     gene            371..757
                     /gene="secE"
                     /locus_tag="VF453_16950"
     CDS             371..757
                     /gene="secE"
                     /locus_tag="VF453_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX7689401.1"
                     /translation="MATNSQIETVQTGADKAKLAGAAVLLLGGIVAFYALERSELWIR
                     VVALLVLVAGAVAVFFTSEPGRQLIGFGKESMKELRKVVWPTRKEALQNTGYVFAFVF
                     TMALFLWITDKVLEWALYDLILGWKR"
     gene            774..1352
                     /gene="nusG"
                     /locus_tag="VF453_16955"
     CDS             774..1352
                     /gene="nusG"
                     /locus_tag="VF453_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX7689402.1"
                     /translation="MTESTQVEEGQGGAANPLRWYVVHAYSGMEKAVERNLRERIDRA
                     GMQAKFGRILVPTEEVVELKNGKKSVTERRFFPGYVLVEMLLDDESWHLVKHTSKVTG
                     FVGGARNRPAPISEAEVMKIVNQMQEGVDKPRPKVEWVVGELVRVKEGPFTDFNGAIE
                     EVNYEKSKVKVSVTIFGRATGVELDFAQVEKV"
     gene            1633..2064
                     /gene="rplK"
                     /locus_tag="VF453_16960"
     CDS             1633..2064
                     /gene="rplK"
                     /locus_tag="VF453_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX7689403.1"
                     /translation="MAKKIVGLIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     KTQGMEPGMKIPVIITAFADKSFTFELKSPPATDLIKKALKLDKGSQRPNTQKVGKIT
                     RAQLEEIAKVKTKDLTGADIDAQIRTIAGTARSMGITVEGL"
     gene            2067..2774
                     /gene="rplA"
                     /locus_tag="VF453_16965"
     CDS             2067..2774
                     /gene="rplA"
                     /locus_tag="VF453_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7689404.1"
                     /translation="MAKTTKRQKALVGKVDSTKLYAIVDALSLVKECATAKFDESIDV
                     AVQLGIDAKKSDQVVRGAVVLPHGTGKTKRVAVFAQGAKAEEARAAGADIVGMEDLAE
                     KVKAGQLDFDVVIASPDTMRVVGTLGQILGPRGLMPNPKVGTVTPDVATAVKNAKAGQ
                     VQFRVDKAGIIHGTIGRRSFESDKLKDNLAALIDALTKAKPASSKGVYLRKVAVSSTM
                     GVGVRVDTTSITAPAAA"
     gene            3167..3697
                     /gene="rplJ"
                     /locus_tag="VF453_16970"
     CDS             3167..3697
                     /gene="rplJ"
                     /locus_tag="VF453_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7689405.1"
                     /translation="MSLNRNEKAAVVEEVAAQVAKSQTLALAEYRGLTVEQMNKLRVD
                     ARAKGVYLHVLKNTLARRAVAGTPFESASESMVGPLIYGFSEDAVAAAKVIADFAKGN
                     DKLVLKGGAYAGKALDVEGVKALANIPSREVLLSQIAMLLQSPIASLARVIDAVEKKK
                     GGGAEAPAAEAAPAAA"
     gene            3753..4130
                     /gene="rplL"
                     /locus_tag="VF453_16975"
     CDS             3753..4130
                     /gene="rplL"
                     /locus_tag="VF453_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX7689406.1"
                     /translation="MAFDKDAFLTSLDSMSVMELNDLVKAIEEKFGVSAAAVAVSGGG
                     GGAAAAAPVEEKTEFNVVLTGAGANKVGVIKAVRELTGLGLKEAKDLVDGAPKNVKEG
                     IAKADAEAAVKKLVEAGATAEMK"
     gene            4421..8542
                     /gene="rpoB"
                     /locus_tag="VF453_16980"
     CDS             4421..8542
                     /gene="rpoB"
                     /locus_tag="VF453_16980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7689407.1"
                     /translation="MAQATPYSFTERKRIRKNFGKRESVLNVPYLLTMQQDSYVAFLQ
                     KDVAPQRRKPEGLQAAFLSAFPIVSHNGMVEMKFIEYNIAKPPFDVRECQQRGLTFAA
                     AVRAKLQMIIYDRESAPAKVVKEIKEQEVYMGEVPLMTDYGSFIVNGTERVIVSQLHR
                     SPGVFFEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDILYFRVDRRRKMPVTIL
                     LKAIGLNPEAILAHFFRNDTFRLMDSGAQMEFVSERLKGEVARFDITDKDGNVIVEKD
                     KRITARHVRQIEQSGTTHITVPEDFLVGRIVARNMIDADTGEIIAKANDELTESLLKK
                     LRGAGVKELQALFTNELDQGAYISQTLATDETADQLAARVAIYRMMRPGEPPTEDAVE
                     ALFNRLFYSDDTYDLSRVGRMKFNARVNRDTADGPMTLSNEDILDVVKILVELRNGRG
                     EVDDIDHLGNRRVRCVGELAENQYRSGLARIEKAVKERLGQAETEALMPHDLINSKPI
                     SAALKEFFGASQLSQFMDQTNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYG
                     RVCPIETPEGPNIGLINSLALYARLNEYGFLETPYRRVVDQKVTDQIDYLSAIEESKY
                     VIAQANASLDATGTLTDELVSAREKGESVLTSPERIQYMDVAPTQIVSVAASLVPFLE
                     HDDANRALMGANMQRQAVPVLRPEKALVGTGVERVAAVDSGTVVTARRGGVVDYIDTN
                     RVVIRVNDAETVAGEVGVDIYNLIKYQRSNQNTNIHQRPIVQRGDKVGAGDVIADGAS
                     TDLGELALGQNMLVAFMPWNGYNFEDSILLSERVIADDRYTSIHIEELVVMARDTKLG
                     SEEITRDIPNLSEQQLGRLDESGIVYIGAEVNAGDVLVGKVTPKGETTLTPEEKLLRA
                     IFGEKASDVKDTSLRVDQGTSGTVIDVQVFTREGIQRDKRAQQIIDDELKRFRLDLND
                     QLRIVEADAFDRIAKLLTGRVANGGPQKIAKGTTIDKAYLASVEKYHWFDIRPAEDEV
                     ANQLESIKNSLEQTRHSFDLAFEEKRKKLTQGDELPAGVLKMVKVYLAVKRRLQPGDK
                     MAGRHGNKGVVSKIVSVEDMPYMADGTPCDIVLNPLGVPSRMNVGQVLEVHLGWAGKG
                     IGQRIGDLLQKQAAVAEIRAFMDELYNKSGGKSERIGELTDNEVVNMAAELSKGVPFA
                     TPVFDGAAEEEIRGMLKLAYPDDIAARKGLTQSRTQCILHDGRTGEAFERPVTVGYMH
                     VLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYVLQE
                     MLTVKSDDVNGRTKVYENIVKGEHAIDAGMPESFNVLVKEIRSLGIDIELEKN"
     gene            8664..12893
                     /gene="rpoC"
                     /locus_tag="VF453_16985"
     CDS             8664..12893
                     /gene="rpoC"
                     /locus_tag="VF453_16985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7689408.1"
                     /translation="MKGLLDLFKQFTPDEHFDAIKIGLASPEKIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRDRM
                     GHIDLAAPCAHIWFLKSLPSRLGLVLDMTLRDIERVLYFEAYVVVDPGMTPLKKFAIM
                     TEDDYEAKHAEFGDEFVALMGAEGIQKLLEEMDLDVEIDKLRNDMTGSELKVKKNSKR
                     LKVMEAFKKSGIKPHWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNR
                     LARLLELKAPEIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRALKSLADMIKGKSG
                     RFRQNLLGKRVDYSGRSVITVGPTLKLHQCGLPKLMAIELFKPFIFSRLEAMGIATTI
                     KAAKKEVEAGTPVVWDILEEVIKEHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLHPL
                     VCAAFNADFDGDQMAVHVPLSIEAQMEARALMLASNNVLFPANGEPSIVPSQDVVLGL
                     YYATRERINGKGEGMVFRDIAEMLRALENGVVEITARINVMLPEWKKDAATGEFVEHV
                     KLTETTVGRALLSEILPKGLPFSNINKALKKKEISRLINTSFRKCGLKETVVFADKLL
                     QNGFRLATRAGISIAIDDMLVPKEKPGLIERAEKEVKEIEQQYVSGLVTAGERYNKVV
                     DIWGKTGDEVGKVMMAQLAKQKVTDRHGKEVDQESFNSIYMMADSGARGSAAQIRQLA
                     GMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRR
                     LVDVTQDLVVTEEDCGTQNGLAMRALVEGGEVIESLRDRVLGRVTAVEVLHPETQETV
                     LTAGELLSEDMLDKLEAAGVDEVKVRTPLTCMTRYGLCARCYGRDLGRGGLINVGEAI
                     GVIAAQSIGEPGTQLTMRTFHIGGAASRAAVASSVDAKSDGNVGFNATMRYVTNGKGE
                     LVVISRSGEIIILDPHGRERERHKVPYGATLNVKADQAIKHGQVLATWDPLTRPIITE
                     FAGRAHFENVEEGVTVAKQVDEVTGLSTLVVIDPKRRGAAKIVRPQVKLLDAQGHEVK
                     IPGTDHSVTIGFQVGALIQIRDGQDLMPGEVLARIPVEGQKTRDITGGLPRVAELFEA
                     RSPKDKGTLAEKTGTVSFGKETKGKVRLQITDPEGIVHEELVPKEKNILVHEGQVVNK
                     GELVVDGPADPQDILRLLGVEELARYIVDEVQDVYRLQGVKINDKHIEVIVRQMLRRV
                     QIVNPGDSAYIIGEQVERSELFGTNDRLRKEDKVPATYSDVLLGITKASLSTDSFISA
                     ASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGTGMAFHQARKAKEDMDETER
                     RQIAMQEAEELAAAQLQQLDAAPAAGGELAE"
     gene            13157..13774
                     /locus_tag="VF453_16990"
     CDS             13157..13774
                     /locus_tag="VF453_16990"
                     /inference="COORDINATES: protein motif:HMM:NF016733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HEX7689409.1"
                     /translation="MTKTSLKLCAAAVLALAGAAAQAAIVTDGNFAEGASAGSFTTVS
                     GGGSIGAWNVTGDSVDLIGNYWQQAPGGSYTVDLDGNGVGGVSQFLTLANGSYYVSFY
                     LSGNPDGGDATKSVLVSAGDASQAFTFTTGSNSHADMGYVRETMKFNIYDGATVLSFD
                     SQDARSYWGPVIGNVAVSAVPESAPLPMLAAGLGVLALLARRRRA"
     gene            14157..>14572
                     /locus_tag="VF453_16995"
     CDS             14157..>14572
                     /locus_tag="VF453_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit II"
                     /protein_id="HEX7689410.1"
                     /translation="MNQARRSTRLAGVLTTSFLAALLSGCDASLLDPKGQIAADEKSL
                     IITATLLMLIVVIPVILMALYFPWKYRQSNRDATYTPNWSYSHRIEAVVWAIPCAIIL
                     VLGVLTWKTTHSLDPYKPLDSKVKPIEIDVVALDWK"
BASE COUNT         2717 a         4859 c         4844 g         2152 t
ORIGIN      
        1 gagatggtga tgccgggcga caacgtgtcg atcacggtca agctgatcgc cccgatcgcg
       61 atggaagaag gcctgcgctt cgccatccgt gaaggcggcc gcaccgtcgg cgccggcgtc
      121 gtcgccaaga tcatcgagta agcgtttcaa ggctgcaggg gtatagctca attggcagag
      181 cgtcggtctc caaaaccgaa ggttgggggt tcgattccct ctgcccctgc caccgaatcg
      241 cgctgaacgc cagcagcgcg ccgagatcgc cagaaagata aggcagcccg cgggtaggaa
      301 acacccggcg ggctttgctc gctaaggtgc tgcgcatttt tgctcgagag acttcaagct
      361 agtgaaagcg atggctacca attcccagat cgaaaccgtc cagacgggcg cggacaaggc
      421 caagctggcc ggcgccgcgg tgctgctgct cggcgggatc gtcgcgttct acgcgctgga
      481 gcgttccgag ctctggatcc gcgtcgtggc gctgctcgtc ctggtggcgg gcgccgtggc
      541 cgtcttcttc acgtccgagc ccggcaggca gctgatcggc ttcggcaagg agtcgatgaa
      601 ggagctgcgc aaggtcgtct ggccgacgcg caaggaggcc ctgcagaaca ccgggtacgt
      661 gttcgccttc gtcttcacga tggcgctgtt cctgtggatc acggacaagg tgctcgagtg
      721 ggcgctgtat gacctgatcc tcggctggaa gcgttgagaa gggttttgtc cgaatgactg
      781 aaagcaccca agtcgaagaa ggccagggcg gcgcggccaa cccgctgcgg tggtacgtcg
      841 tccacgccta ctcgggcatg gagaaggccg tcgagcgcaa cctgcgcgag cgcatcgacc
      901 gcgccggcat gcaggccaag ttcggccgca tcctggtgcc gaccgaggag gtcgtcgagc
      961 tgaagaacgg caagaagtcg gtgaccgagc gccgcttctt tcccggttac gtgctggtcg
     1021 agatgctgct ggacgatgaa tcctggcacc tggtcaagca caccagcaag gtcaccggct
     1081 tcgtcggcgg cgcgcgcaat cgcccggccc cgatctcgga agccgaggtg atgaagatcg
     1141 tcaaccagat gcaggaaggc gtcgacaagc cccgtcccaa ggtggagtgg gtggtcggcg
     1201 aactcgtccg cgtcaaggaa ggcccgttca ccgacttcaa cggcgcgatc gaggaagtca
     1261 actacgagaa gtccaaggtc aaggtctcgg tcacgatctt cggtcgtgcc acgggcgtcg
     1321 agctggattt cgcgcaggtc gagaaggttt gaatttcccg ctgccgcgcc gggcgctcgt
     1381 gctcggccgt ggcggcggcg cagcgccgat ctccccggta ggccttctgg ccggccgggg
     1441 gttttgcgtc tgtgggataa tgcttggttc gcccgacggg gggcgctggg cggcgcaagc
     1501 cggcctgaac cccgtccgtt gctgccggtg gcgagacacc ggttcgagga gcgcaggtag
     1561 ccacccctgt gcgttggaac tcaacgatcc cgccatggcg ccgccggcgc cgggatcgct
     1621 ctggagaaac acatggcaaa gaagattgtc ggcttgatca agctgcaaat cccggccggc
     1681 aaggcaaacc cgtcgccgcc ggtgggcccg gccctcggcc agcgcggcct gaacatcatg
     1741 gaattctgca aggcgttcaa cgccaagacg cagggcatgg agccggggat gaagatcccc
     1801 gtcatcatca ccgcgttcgc ggacaagtcg ttcacgttcg agctgaagtc gccgccggcg
     1861 accgacctga tcaagaaggc cctgaagctc gacaagggct cgcagcgtcc caacacgcag
     1921 aaggtcggca agatcacccg cgcccagctg gaagagatcg ccaaggtcaa gaccaaggac
     1981 ctgaccggcg ccgacatcga cgcccagatc cgtaccatcg ccggcaccgc ccgctcgatg
     2041 ggcatcaccg tggaagggct ctgatcatgg ccaagaccac caagcgccag aaggccctcg
     2101 tgggcaaggt cgactcgacc aagctgtacg ccatcgtcga cgcgctgtcg ctcgtcaagg
     2161 aatgcgcgac cgccaagttc gacgagtcga tcgacgtcgc cgtccagctc ggcatcgatg
     2221 cgaagaagtc cgaccaggtc gtccgcggcg ccgtcgtgct gccgcacggc accggcaaga
     2281 ccaagcgcgt cgccgtgttc gcccagggcg ccaaggctga agaagcccgc gccgccggcg
     2341 ccgacatcgt cggcatggaa gacctggccg agaaggtcaa ggccggccag ctcgacttcg
     2401 acgtcgtgat cgcctcgccc gacacgatgc gcgtcgtcgg tacgctgggc cagatcctcg
     2461 gcccgcgcgg cctgatgccg aacccgaagg tcggcacggt caccccggac gtcgccacgg
     2521 ccgtcaagaa cgccaaggcc ggccaggtgc agttccgcgt cgacaaggcc ggcatcatcc
     2581 atggcacgat cggccgccgc tcgttcgagt cggacaagct gaaggacaac ctggccgcgc
     2641 tgatcgacgc gctgaccaag gccaagccgg cgtcgagcaa gggtgtgtac ctgcgcaagg
     2701 tcgccgtctc gtcgaccatg ggcgtgggcg tccgcgtgga caccacgtcg atcacggccc
     2761 ccgcggccgc ctgatcggca ctctggattg aagccgtccg ccgcttgggt cttgagcggc
     2821 gagtcggtgg caaggattgg tgggctgtcc gcccgtgtcc cccgggacgt gaggcgggca
     2881 ggccatccaa gaccgctggc cggggcctct cccgcagccg cgcgcgagcg cggcgagggg
     2941 gcggctcctt aaaccggccc ggaagcccgt cgtcgacgga cacccggatc gagccagcgc
     3001 agatggcgaa cccgccggaa ctgatgaagc agtggaatgc gtggacgtcg acctccgggc
     3061 cggcgcgagc ggcaagaagc ggtggcagca gtgtcggcaa ggtcgctgaa cccggtgtgc
     3121 gggacggctc cggcccgaac gcacgtttga acgtgaggag cagaccttga gtctcaatcg
     3181 caacgagaag gcggcagtcg tcgaggaagt ggcagcgcag gttgccaagt cccagacgct
     3241 ggctctggcc gagtaccgtg gcctcaccgt cgagcaaatg aacaagctgc gcgtcgacgc
     3301 gcgggccaag ggtgtgtacc ttcacgtgct gaagaacacg ctggcccgcc gcgccgtcgc
     3361 cggcacgccg ttcgagagcg cgtcggagag catggtcggt ccgctgatct atggtttctc
     3421 ggaagacgcg gtcgccgcgg cgaaggtcat cgcagacttc gccaagggca acgacaagct
     3481 ggttctgaag ggcggcgcct acgccggcaa ggccctggac gtcgaaggtg tgaaggccct
     3541 ggccaacatc ccgtcgcgcg aagtcctgct gtcgcagatc gcgatgctgc tgcagtcgcc
     3601 catcgcttcg ctcgcccgcg tcatcgacgc cgtcgagaag aagaagggcg gcggcgccga
     3661 agcgcccgca gcggaagccg ctccggccgc cgcctgattc caggccggtc gaatacagac
     3721 actcatttca ctgatttaga taggaatcca aaatggcatt cgataaggac gctttcctga
     3781 cctccctcga cagcatgtcc gtcatggagc tgaacgacct ggtcaaggcg atcgaagaga
     3841 agttcggcgt gtcggccgcg gccgtggccg tgtcgggcgg cggcggcggc gctgccgcgg
     3901 cggctccggt cgaagagaag accgagttca acgtcgtgct gaccggcgcg ggcgccaaca
     3961 aggtcggcgt catcaaggcc gttcgcgaac tgacgggcct gggcctgaag gaagcgaagg
     4021 acctggtcga cggcgcgccg aagaacgtca aggaaggcat cgccaaggcc gacgccgagg
     4081 ctgccgtcaa gaagctggtc gaagccggcg ccaccgccga gatgaagtaa tcggcgagga
     4141 cttgcggggc ggcctggccg tccccatgtc ctctcgttcg ctcccgctcc ggcgggagag
     4201 agaacagcgc gaagcgggcg acgatgcccg gcccgtttcg cagtgttctc tgatccgact
     4261 gcagagaaca ggtttggtcg gactccggtg caacgccggg gttgtccgcc agcggttggt
     4321 agtggccaac caccaagcat ccggcgcaac accggagtag ccagtcgccg acgagacgcg
     4381 caaccacggc caggctgcgc gtcgtcgcta cggagtctct atggcgcaag caacccccta
     4441 cagcttcacc gaacgcaagc ggatccgcaa gaacttcggc aagcgcgaga gcgttctcaa
     4501 cgtcccctac ctgctgacga tgcagcagga ttcctacgtc gcgttcctcc agaaggacgt
     4561 ggcgccgcag cgtcgcaagc ccgaggggct gcaggcggcg ttcctctcgg cgttcccgat
     4621 cgtgtcgcac aacggcatgg tcgagatgaa gttcatcgaa tacaacatcg ccaagccgcc
     4681 gttcgacgtc cgcgagtgcc agcagcgcgg cctcaccttc gccgccgcgg tgcgcgcgaa
     4741 gctgcagatg atcatctatg accgtgagtc ggcgccggcc aaggtggtca aggagatcaa
     4801 ggagcaggag gtctacatgg gcgaagtgcc gctcatgacc gactacggct ccttcatcgt
     4861 caacggcacc gagcgcgtca tcgtcagcca gctgcaccgt tcgcccggcg tgttcttcga
     4921 gcacgacaag ggcaagacgc attcgtccgg caagctgctg ttctcggcgc gcatcatccc
     4981 ctaccgcggc tcgtggctgg acttcgagtt cgacccgaag gacatcctgt acttccgcgt
     5041 cgaccgccgc cgcaagatgc cggtgacgat cctgctgaag gccatcggcc tgaacccgga
     5101 agcgatcctc gcgcacttct tccggaacga caccttccgc ctgatggact cgggcgcgca
     5161 gatggaattc gtctccgagc gcctgaaggg cgaggtcgcc cgcttcgaca tcaccgacaa
     5221 ggacggcaac gtcatcgtcg agaaggacaa gcgcatcacc gcgcgccacg tgcgccagat
     5281 cgagcagtcc ggcaccacgc acatcacggt gcccgaggac ttcctcgtcg gccgcatcgt
     5341 cgcgcgcaac atgatcgacg ccgacacggg cgagatcatc gccaaggcca acgacgagct
     5401 caccgagtcg ttgctgaaga agctgcgcgg cgccggcgtg aaggagctgc aggccctgtt
     5461 cacgaacgag ctggaccagg gtgcctacat cagccagacg ctcgcgaccg acgagacggc
     5521 cgaccagctc gccgcccgcg tcgcgatcta ccgcatgatg cgccccggcg agccgccgac
     5581 cgaggacgcg gtcgaggccc tgtttaaccg cctgttctac agcgacgaca cgtacgatct
     5641 ctcgcgagtc ggccgcatga agttcaacgc gcgcgtcaac cgcgacacgg ccgacggccc
     5701 gatgacgctg tcgaacgagg acatcctcga cgtcgtcaag atcctcgtcg agctgcgcaa
     5761 cggccgcggc gaggtggacg acatcgacca cctcggcaac cgccgcgtgc gctgcgtcgg
     5821 cgagctggcc gagaaccagt accgctccgg cctcgcgcgc atcgagaagg ccgtcaagga
     5881 gcgcctcggc caggccgaga ccgaggcgct gatgccgcac gacctgatca actccaagcc
     5941 gatttccgcg gcgctcaagg agttcttcgg ggcgtcgcag ctgtcgcagt tcatggacca
     6001 gaccaacccg ctgtccgaga tcacgcacaa gcgtcgcgtc tcggcgctgg gcccgggcgg
     6061 cctgacgcgc gagcgcgccg gcttcgaggt ccgcgacgtg cacccgacgc actacggccg
     6121 cgtctgcccg atcgagacgc cggaaggccc gaacatcggc ctgatcaact cgctggccct
     6181 gtacgcccgc ctgaacgaat acggcttcct cgagacgccg taccgccgcg tggtcgacca
     6241 gaaggtcacc gaccagatcg actacctgtc ggccatcgag gagtcgaagt acgtgatcgc
     6301 gcaggcgaac gcctcgctcg acgccaccgg cacgctgacc gacgagctcg tgtccgcgcg
     6361 cgagaagggc gaatccgtgc tgacctcgcc ggagcgcatc cagtacatgg acgtcgcgcc
     6421 gacgcagatc gtctcggtcg ccgcctcgct ggtgcccttc ctcgagcacg acgacgcgaa
     6481 ccgcgcgctg atgggcgcga acatgcagcg ccaagccgtc ccggtcctgc gtcctgaaaa
     6541 ggcgctggtc ggcacgggcg tggagcgcgt ggccgcggtc gactcgggca cggtcgtcac
     6601 cgcgcgccgc ggcggcgtgg tcgactacat cgacaccaac cgcgtcgtca tccgcgtcaa
     6661 cgacgccgag acggtggcgg gcgaggtcgg cgtcgacatc tacaacctga tcaagtacca
     6721 gcgttccaac cagaacacga acatccacca gcgtcccatc gtccagcgcg gcgacaaggt
     6781 cggcgcgggc gacgtgatcg cggacggcgc gtcgacggac ctgggcgagc tcgcgctcgg
     6841 ccagaacatg ctcgtcgcgt tcatgccctg gaacggctac aacttcgaag actcgatcct
     6901 gctgtccgag cgcgtcatcg ccgacgaccg ctacacctcg atccacatcg aggagctggt
     6961 ggtcatggcc cgcgacacca agctgggctc cgaggaaatc acgcgcgaca tcccgaacct
     7021 gtcggaacag cagctgggcc gcctggacga gtccggcatc gtctacatcg gtgccgaggt
     7081 caatgcgggc gacgtgctcg tgggcaaggt cacgcccaag ggcgagacca cgctgacgcc
     7141 ggaagagaag ctgctgcgcg cgatcttcgg cgagaaggcg tccgacgtga aggacacgtc
     7201 gctgcgcgtc gaccagggca cgagcggcac cgtcatcgac gtgcaggtct tcacccgcga
     7261 aggcatccag cgcgacaagc gcgcccagca gatcatcgac gacgagctga agcgcttccg
     7321 cctggacctc aacgaccagc ttcgcatcgt cgaggccgac gcgttcgacc ggatcgccaa
     7381 gctcctgacc ggccgcgtgg ccaatggcgg cccgcagaag atcgccaagg gcacgacgat
     7441 cgacaaggcc tacctggcca gcgtcgagaa gtaccactgg ttcgacatcc gtccggccga
     7501 ggacgaggtg gccaaccagc tcgagtcgat caagaactcg ctcgagcaga cgcgccacag
     7561 cttcgacctc gccttcgagg agaagcgcaa gaagctgacg cagggcgacg agctgcccgc
     7621 gggcgtgctg aagatggtca aggtctacct ggccgtcaag cgccgcctgc agcctggcga
     7681 caagatggcc ggccgccacg gcaacaaggg cgtggtctcc aagatcgtct cggtcgagga
     7741 catgccctac atggccgacg gcacgccgtg cgacatcgtg ctgaacccgc tgggcgtccc
     7801 gtcgcggatg aacgtgggcc aggtgctcga ggtgcacctg ggctgggccg gcaagggcat
     7861 cggccagcgc atcggcgacc tgctgcagaa gcaggcggcg gtggccgaga tccgcgcgtt
     7921 catggacgag ctctacaaca agtcgggcgg caagtcggag cgcatcggcg agctgacgga
     7981 caacgaggtc gtcaacatgg cggccgagct gagcaagggc gtgcccttcg cgacgccggt
     8041 gttcgacggc gcggctgaag aagagatccg cggcatgctc aagctcgcat acccggacga
     8101 catcgccgcc cgcaaggggc tcacgcagtc gcgcacccag tgcatcctgc acgacggtcg
     8161 caccggcgaa gccttcgagc gcccggtgac cgtcggctac atgcacgtgc tgaagctgca
     8221 ccacctggtc gacgacaaga tgcacgcgcg ctccacgggc ccgtactcgc tcgtcacgca
     8281 gcagccgctc ggcggcaagg cgcagttcgg cggccagcgc ttcggcgaga tggaagtctg
     8341 ggcgctggaa gcctacggcg cggcctacgt gctgcaggag atgctgacgg tcaagtccga
     8401 cgacgtgaac ggccgcacga aggtgtacga gaacatcgtc aagggcgagc acgccatcga
     8461 cgccgggatg ccggagtcgt tcaacgtgct ggtcaaggaa attcgctcgc tcggcatcga
     8521 catcgagctg gagaaaaatt gacccccccg aagcgacctt cggtcgcctc cccccgaggg
     8581 ggcgacccca gcggctcggc aaagccggtt ccgcggcgtc cgcttggctt gacggccacg
     8641 tattcagttt ttaggagaga tgcatgaagg gcttgctgga ccttttcaag caattcaccc
     8701 ccgatgagca tttcgatgcc atcaagatcg gcctcgcgtc gcccgagaag atccgctcgt
     8761 ggtctttcgg cgaagtgaag aagccggaga ccatcaacta ccggacgttc aagccggagc
     8821 gcgatggcct gttctgcgcc aagatcttcg ggccgatcaa ggactacgag tgcctgtgcg
     8881 gcaagtacaa gcgcctgaag caccgcggcg tcatctgcga gaagtgcggc gtcgaagtga
     8941 cccagaccaa ggtccgccgt gaccgcatgg gccacatcga cctggcggcg ccgtgcgcgc
     9001 acatctggtt cctgaagtcg ctgccgtcgc gcctgggcct cgtgctcgac atgacgctgc
     9061 gcgacatcga gcgcgtgctg tacttcgaag cctacgtggt cgtcgacccg ggcatgacgc
     9121 cgctgaagaa gttcgcgatc atgaccgagg acgactacga ggcgaagcac gccgagttcg
     9181 gcgacgagtt cgtcgcgctg atgggcgccg agggcatcca gaagctgctg gaggaaatgg
     9241 atctcgacgt cgagatcgac aagctccgca acgacatgac gggctcggag ctcaaggtca
     9301 agaagaactc caagcgcctg aaggtcatgg aggccttcaa gaagtcgggc atcaagcccc
     9361 actggatgat catggacgtc ctgccggtgc tgccgccgga cctgcgtccg ctggtgccgc
     9421 tggacggcgg ccgcttcgcg acctccgacc tgaacgacct ctatcgccgc gtcatcaacc
     9481 gcaacaaccg tctcgcgcgc ctgctggagc tgaaggctcc ggagatcatc gtgcgcaacg
     9541 agaagcggat gctgcaggag gcggtggact cgctgctgga caacggccgt cgcggcaagg
     9601 cgatgaccgg cgcgaacaag cgcgccctga agtcgctcgc cgacatgatc aagggcaagt
     9661 cgggccgctt ccgccagaac ctgctgggca agcgcgtcga ctactccggc cgttccgtca
     9721 tcaccgtggg tccgacgctc aagctgcacc agtgcggcct gcccaagctg atggccatcg
     9781 agctcttcaa gccgttcatc ttctcgcgcc tcgaggccat gggcatcgcg acgacgatca
     9841 aggcggccaa gaaggaagtc gaagccggca cgccggtggt gtgggacatc ctggaagagg
     9901 tcatcaagga gcacccggtg atgctgaacc gggcgcccac gctgcaccgc ctcggcatcc
     9961 aggccttcga gcccgtgctg atcgaaggca aggcgatcca gctgcacccg ctcgtctgcg
    10021 cggcgttcaa cgccgacttc gacggcgacc agatggccgt ccacgtgccg ctgtcgatcg
    10081 aggcgcagat ggaagcgcgc gcgctgatgc tcgcctccaa caacgtgctg ttcccggcca
    10141 acggcgaacc gtcgatcgtg ccgtcgcagg acgtcgtgct gggcctgtac tacgccacgc
    10201 gcgagcgcat caacggcaag ggcgagggca tggtcttccg cgacatcgcg gagatgctgc
    10261 gcgcgctcga gaacggcgtg gtcgagatca cggcccgcat caacgtgatg ctgcccgagt
    10321 ggaagaagga cgcggcgacg ggcgagttcg tcgagcacgt caagctgacc gagacgacgg
    10381 tcggccgcgc gctgctgtcc gagatcctgc ccaagggcct gccgttctcg aacatcaaca
    10441 aggcgctgaa gaagaaggag atctcgcgcc tgatcaacac gtcgttccgc aagtgcggcc
    10501 tgaaggagac ggtcgtcttc gcggacaagc tgctgcagaa cggcttccgc ctggccacgc
    10561 gcgccggcat ctcgatcgcc atcgacgaca tgctggtccc gaaggagaag cccggcctga
    10621 tcgagcgcgc cgagaaggag gtcaaggaga tcgagcagca gtacgtctcg ggtctcgtga
    10681 cggccggcga gcgctacaac aaggtcgtcg acatctgggg caagaccggt gacgaggtgg
    10741 gcaaggtcat gatggcccag ctcgccaagc agaaggtcac cgaccgccac ggcaaggaag
    10801 tcgaccagga gtcgttcaac tcgatctaca tgatggccga ctccggcgcc cgcggctccg
    10861 cggcgcagat ccgccagctg gccggcatgc gcggcctgat ggccaagccc gacggctcca
    10921 tcatcgagac gccgatcacg gcgaacttcc gcgaaggcct gaacgttctc cagtacttca
    10981 tctccaccca cggcgcccgc aagggcctgg cggacacggc gctgaagacc gcgaactccg
    11041 gctacctgac gcgtcgtctc gtcgacgtca cgcaggacct ggtcgtcacc gaggaggact
    11101 gcggcacgca gaacggcctc gcgatgcgcg ccctggtcga aggcggcgaa gtcatcgagt
    11161 cgctgcgcga ccgcgtcctc ggccgcgtga ccgccgtcga ggtgctgcac cccgagaccc
    11221 aggagaccgt gctgaccgcg ggcgagctgc tgtcggaaga catgctcgac aagctcgagg
    11281 ctgccggcgt cgacgaggtc aaggtgcgca cgccgctgac ctgcatgacg cgctacggcc
    11341 tgtgcgcgcg ctgctacggc cgcgacctcg gccgcggcgg cctgatcaac gtgggcgagg
    11401 cgatcggcgt gatcgcggcc cagtcgatcg gcgagcccgg cacgcagctg acgatgcgca
    11461 cgttccacat cggcggcgcg gcctcgcgcg cggcggtggc gtccagcgtc gacgcgaagt
    11521 cggacggcaa cgtcggcttc aacgcgacga tgcgctacgt gacgaacggc aagggcgagc
    11581 tcgtggtcat ctcccgttcg ggcgagatca tcatcctcga tccgcacggc cgcgagcgcg
    11641 agcgccacaa ggtgccctac ggcgccacgc tgaacgtgaa ggccgaccag gcgatcaagc
    11701 acggccaggt gctcgcgacc tgggatccgc tgacccgccc catcatcacg gaattcgccg
    11761 gccgcgccca cttcgagaac gtcgaggagg gcgtgaccgt cgcgaagcag gtcgacgagg
    11821 tcaccggcct gtcgacgctg gtcgtgatcg acccgaagcg tcgcggcgcc gccaagatcg
    11881 tgcgtccgca ggtcaagctg ctcgacgcgc aaggccacga agtcaagatc cccggcaccg
    11941 accactcggt gacgatcggc ttccaggtcg gcgcgctgat ccagatccgc gacgggcagg
    12001 acctgatgcc cggcgaagtg ctcgcccgca tcccggtcga aggccagaag acgcgcgaca
    12061 tcacgggcgg tctgccgcgc gtggcggagc tgttcgaggc ccgcagcccg aaggacaagg
    12121 gcacgctggc cgagaagacc ggcacggtct cgttcggcaa ggagaccaag ggcaaggtgc
    12181 gcctgcagat caccgatccc gaaggcatcg tccacgagga actggtgccc aaggagaaga
    12241 acatcctggt gcacgagggc caggtggtca acaagggcga gctggtcgtc gacggcccgg
    12301 ccgatccgca ggacatcctg cgcctgctgg gcgtcgagga actggcgcgc tacatcgtcg
    12361 acgaggtgca ggacgtctac cgcctccagg gcgtgaagat caacgacaag cacatcgagg
    12421 tgatcgtccg ccagatgctg cgccgcgtgc agatcgtcaa cccgggcgac agcgcctaca
    12481 tcatcggcga gcaggtcgag cgttcggagc tgttcggcac gaacgaccgc ctgcgcaagg
    12541 aggacaaggt gcccgcgacc tactccgacg tgctgctggg catcacgaag gcgtcgctgt
    12601 cgacggactc gttcatctcg gccgcttcct tccaggaaac gacccgcgtg ctcaccgagg
    12661 cggccatcat gggcaagcgc gacgagcttc gcggcctgaa ggagaacgtc atcgtcggtc
    12721 gcctgatccc ggctggcacg ggcatggcct tccaccaggc ccgcaaggcc aaggaagaca
    12781 tggacgagac cgagcgccgc cagatcgcca tgcaggaggc cgaggaactg gccgccgcgc
    12841 aactccagca gctggacgcc gcacccgctg ccggcggcga actggccgag tgacgcaccc
    12901 gccgacctcg cgccgcacgg cgccggtcgt cgcctccaaa aggcgctccg cacggggcgc
    12961 cttttttcat gcctgcgcga tcgtcggacc tgcgcccgac gcgggcatct cggcacgcgc
    13021 ggcgcggggt tgagcgcggc gaggcctcat ttcagccaac aagtgcacac gggcgatgac
    13081 ggcgtggctg gccgcgctgc ccggccgacg atggcgttga tacagtatgt tgcaaatgaa
    13141 ccgacaaggc agctccatga ccaagacctc cctcaagctc tgcgccgcgg ccgtcctggc
    13201 cctggctggc gccgccgccc aggcggccat cgtcaccgac ggcaacttcg cggaaggcgc
    13261 ctccgccggc agcttcacca cggtgagcgg cggcggctcc atcggcgcct ggaacgtgac
    13321 cggcgactcc gtcgacctga tcggcaacta ctggcagcag gcgcccggcg gcagctacac
    13381 ggtcgacctc gatggcaacg gcgtgggcgg cgtgtcgcag ttcctcacgc tggccaacgg
    13441 cagctactac gtcagcttct acctgtccgg caatccggat ggcggcgatg ccaccaagtc
    13501 cgtgctcgtc agcgccggcg acgcgtcgca ggctttcacg ttcaccaccg gctcgaactc
    13561 ccacgccgac atgggctacg tgcgcgagac gatgaagttc aacatctacg acggcgcgac
    13621 cgtgctgtcc ttcgacagcc aggatgcccg cagctactgg gggccggtga tcggcaacgt
    13681 cgccgtctcc gccgttccgg aatcggcgcc gctgccgatg ctggcggccg gcctgggcgt
    13741 gctggcgctt ctcgcgcgcc gccgccgcgc ctgaaccttc ccggcccgcc gtgcgcgggc
    13801 agggacgcgg cagggccgat cccgcgggat cggccctttt cctttgcgcc ggcgcgcgcg
    13861 ctcgtccggt gcaaatcgat ttgtgggcga tgcaacagcc gtccgccggg ccagctgggc
    13921 cgggtgcgag gcttgctcgc gccatcgacg gtctactcga tggcaacgtc cggcaactcg
    13981 cgcggcggcg aggcccgcac tcgggtgcag gctctagaaa taccccaggc cgcgggtcga
    14041 ttgggcgggc gttcgcctgc aaagtttggc aaaatcttca gattaacccc aactgccccc
    14101 ccgcgcctgc ctggatacct tggtgggctg acccgtgggc gcccgccgag agggaaatga
    14161 accaagcccg cagaagtaca cgcctcgcag gcgtgctgac gacgtcgttc ctggctgcgc
    14221 tgttgagcgg ctgcgacgcg tcactcctcg accccaaggg tcagatcgcc gccgacgaga
    14281 agtcgctgat catcaccgcg acgctgctga tgctgatcgt ggtgatcccg gtgatcctga
    14341 tggcgctcta cttcccgtgg aagtaccgcc agtcgaaccg ggacgcgacc tacacgccca
    14401 actggtcgta ctcgcaccgc atcgaggccg tcgtctgggc catcccctgc gcgatcatcc
    14461 tggtgctggg cgtgctgacg tggaagacga cgcactcgct cgacccgtac aagccgctgg
    14521 attccaaggt caagccgatc gagatcgacg tcgtcgcgct ggactggaag tg
//
LOCUS       DASVLN010000721         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_147673, whole genome shotgun sequence.
ACCESSION   DASVLN010000721 DASVLN010000000
VERSION     DASVLN010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147673"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..736)
                     /locus_tag="VF453_17000"
     CDS             complement(62..736)
                     /locus_tag="VF453_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7689411.1"
                     /translation="MNRIALVEDHDRLAALVRQALGGAGIEVDVFERMDAAWAALGLA
                     PYGAVVVDRGLPDGDGLELVRRLRAEGRVVPCLMLTARDALHDRVDGLESGADDYLAK
                     PFPMEELVARVRALLRRPAAMQSLSPRFGDVRVEPDAGCMACADEQVTLPAAELQIML
                     CLVRAEGRTVRRAALEAAAWGLSDAVTPNALDVALHRLRRKLAAIGSTLQIANARGHG
                     YGLREG"
     gene            complement(786..1604)
                     /locus_tag="VF453_17005"
     CDS             complement(786..1604)
                     /locus_tag="VF453_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689412.1"
                     /translation="MSLNPSSPAPGVRLDLPSLAGSDAAAARSSAGRPGLLGQGLLRL
                     LTHRATVGAVHELSSTLRLVSLRAASFRDLAEVPGDKVQLRVSGMSFRTFTPFRLGGP
                     ADELLVLGHVHGWGPGSDWLGGVAPGEPCHVLGPRRSLDLGTIQRSTVFFGDETSLGL
                     ARALCSTPLGAVDTHFIFETGDAYGMRQALEALGEGMLRNAWLVERRAVDGHLPEIEA
                     AFARYAAADAYRQYVLSGRAPAVQRLVRGLRGAGAKPSQVLAKAYWAPGKVGLD"
     gene            1900..>2182
                     /locus_tag="VF453_17010"
     CDS             1900..>2182
                     /locus_tag="VF453_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689413.1"
                     /translation="MSNKKRLRAAILLLIALMLAAAMFHGEIDGFWHRHQAHPMPPAA
                     LASAGAGAGHGATPVATAPLPRDTSAPVAFGAHRVRVAHVKAEGGAGVAA"
BASE COUNT          305 a          804 c          812 g          261 t
ORIGIN      
        1 ctcgtgccat tcctcgtagg cctggcgacg cgcggcgagc atcccggccc aggcgtcgaa
       61 cctacccttc acggagccca tagccgtggc ctcgcgcgtt ggcgatctgg agggtcgagc
      121 cgatcgccgc gagcttgcgc cgcagccggt gcagcgccac gtccagcgcg ttgggcgtga
      181 ccgcatcgga cagcccccag gccgcggcct cgagcgccgc gcggcgcacg gtgcggccct
      241 cggcccgcac gaggcacagc atgatctgca gctccgcggc gggcagcgtc acctgttcgt
      301 cggcgcaggc catgcagccg gcgtcgggct cgacgcgcac gtcgccgaag cgcggcgaca
      361 ggctctgcat cgccgccggc cggcgcagga gcgcgcgcac gcgggcgacg agctcctcca
      421 tcgggaaggg cttggcgagg tagtcgtcgg cgccggactc gaggccgtcg acgcggtcgt
      481 gcagcgcatc gcgcgccgtc agcatcaggc acggcacgac acgcccctcg gcgcgcagcc
      541 ggcgcacgag ttccagcccg tcgccgtccg gcaggccgcg gtcgaccacc acggcaccgt
      601 atggcgcaag cccgagcgcg gcccaggccg catccatgcg ctcgaacacg tcgacctcga
      661 tgccggcgcc gcccagcgcc tggcgcacga gggcggcgag gcggtcgtgg tcttcgacga
      721 gggcgatgcg gttcatgcgg acaggcagcg taccagtctc gaggatctgc gaatcagcgc
      781 cgcgctcaat ccaggccgac cttgcccggc gcccagtacg ccttcgcgag cacctgagag
      841 ggcttggccc ccgccccgcg caggccgcgc acgaggcgct gcaccgccgg cgcgcggccg
      901 ctcaggacgt actggcggta tgcatcggcc gcggcgtagc gcgcgaacgc ggcctcgatc
      961 tccggcaggt ggccgtcgac ggcgcggcgc tcgacgagcc aggcgttgcg cagcatgcct
     1021 tcgccgagcg cctcgagcgc ctggcgcatg ccgtaggcgt cgccggtctc gaagatgaag
     1081 tgggtgtcga cggcgcccag gggcgtgctg cacagcgcgc gcgccaggcc gagcgaggtc
     1141 tcgtcgccga agaagacggt ggagcgctgg atcgtgccca ggtccagcga gcgccgcggc
     1201 ccgaggacgt ggcacggctc gccgggcgcg acgccgccca gccagtccga gccggggccc
     1261 cagccgtgga cgtggccgag gacgagcagc tcgtcggccg gcccgccgag gcgaaacggc
     1321 gtgaaggtgc ggaagctcat gccggagacg cgcagctgca ccttgtcgcc cggcacctcc
     1381 gcgaggtcgc ggaagctggc ggcgcgcagg ctgacgagcc gcagcgtgga cgacagctcg
     1441 tgcaccgcgc cgaccgtggc gcggtgggtg agcaggcgca gcaggccctg gccgaggagg
     1501 ccggggcggc cggcggacga acgggcggcg gccgcgtcgc tgccggccag ggacggaagg
     1561 tcgaggcgca cgccgggcgc cgggctgctc gggttcaggg acatggggcg gcctttcgcg
     1621 gtggattcga tcgacgcgga tcgtcccgcc gcccggcttt ccggttcctt accgccgcga
     1681 ttcccgcagc gccccagggt catcccggac cgccgcgacg gccaccgtcc ggcgggaggc
     1741 gttcccacca tccggtgccc atcggcagcg cgcgcgccgg ctttagcccg gatgcgcaaa
     1801 ctagttcacg caatcggggg caagtcctgc cgctcgaccg tgatgcgagg cagctcggcc
     1861 gcagtaacat caggtaactc ctcaggttgc aaaaattaca tgtccaacaa gaagcgactc
     1921 cgcgccgcga tcctgctgct gatcgcgctc atgctcgccg cggcgatgtt ccatggggag
     1981 atcgacgggt tctggcatcg ccatcaggcg caccccatgc cgccggccgc cctggccagc
     2041 gcaggagcgg gtgccggcca cggcgcgacc cccgtcgcaa cggcgccgct gccgcgcgac
     2101 acttccgcgc cggtcgcctt cggcgcgcac cgcgtccggg tggcgcacgt gaaggcggaa
     2161 ggcggcgcag gcgtcgcggc gc
//
LOCUS       DASVLN010000722         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_412761, whole genome shotgun sequence.
ACCESSION   DASVLN010000722 DASVLN010000000
VERSION     DASVLN010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412761"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..741
                     /locus_tag="VF453_17015"
     CDS             232..741
                     /locus_tag="VF453_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689414.1"
                     /translation="MNPTPAQVLDHVKAGRLARLLRGELAVFTTPAEINADMRPTDHA
                     QLLEAGLYPLVPTPLRPEVLSSLLQQALLDIAVDARGVFCAVQCFYIEIVNEDGGRSP
                     LRLDRDALFASLHAAFLAHEQDLHALAFRPRDLVLDRCHKLTAAAFRVLARDHGLTLP
                     GGAPVAGQA"
     gene            complement(778..1200)
                     /locus_tag="VF453_17020"
     CDS             complement(778..1200)
                     /locus_tag="VF453_17020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689415.1"
                     /translation="MNDLTSPPSPLPAARVLLVEDNAVNRMLATRMLEMLGVAVLEAH
                     DGEEALDCLHGPDGATVAAVLMDCQMPGIDGFEATRRWRAHEAARGGARLPIVALTAN
                     VLPDDIAECKAAGMDAHLGKPFSIADLREVLQPVLAAG"
     gene            1317..>1720
                     /locus_tag="VF453_17025"
     CDS             1317..>1720
                     /locus_tag="VF453_17025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7689416.1"
                     /translation="MPAIPLTIRPFRAGDESPLRAVYASAIHEVARRDYTQAQLDAWA
                     PANFDAENWARRIQGIAPFVAEGADGLLLGYADVQPDGYVDHFFVAGHAGGQGVGGAL
                     MRRLLERARELGVAQLSSDVSRTAQPFFARFG"
BASE COUNT          209 a          659 c          621 g          231 t
ORIGIN      
        1 ggcggcacgg tcacggtcgt cgagctcccc aagcccgctc ccgcggcgac gccggcggcg
       61 ctggcggcga gcctggccgg cggccgcgtg ctccgggaga tggatccggc cgagcgcgac
      121 cgcctgcgcg acaaggagat cgacgacgcg atgaaccggc gctgagcgcc cgtgccgccg
      181 ggtcgacccg gcgccgtcgc cgcgggtcga tccgaacgtc caggtattca aatgaacccg
      241 acccccgcgc aagtcctcga ccacgtgaag gccggccgcc tcgcgcgcct gctgcgcggc
      301 gagctcgcgg tcttcacgac cccggccgag atcaacgcgg acatgcggcc gaccgaccac
      361 gcgcagctgc tcgaggccgg gctctacccg ctcgtgccga caccgctgcg cccggaggtg
      421 ctgtccagcc tgctgcagca ggcgctgctg gacatcgcgg tcgacgcccg cggcgtgttc
      481 tgcgccgtgc agtgcttcta catcgagatc gtgaacgagg acggcgggcg ctcgccgctg
      541 cggctggatc gcgacgcgct gttcgcgagc ctgcacgcgg ccttcctcgc gcacgagcag
      601 gatctccacg cgctggcctt ccgtccgcgc gacctcgtgc tcgatcgctg ccacaagctg
      661 accgcggccg ctttccgcgt cctcgcgcgc gaccacggcc tgacgctgcc gggcggcgcg
      721 ccggtggccg gccaggcctg agagcggtcg cctcgttgcg cgggcgtcgc cggcgcgtca
      781 gcccgcggcc agcaccggct gcagcacctc gcgcaggtct gcgatcgaga acggcttgcc
      841 caggtgcgcg tccatgccgg cggccttgca ctcggcgatg tcgtcgggca gcacgttggc
      901 ggtcagcgcc acgatcggca ggcgcgcgcc gccgcgggcc gcctcgtgcg cgcgccagcg
      961 gcgcgtggcc tcgaagccgt cgatgccggg catctggcag tccatcagga ccgcggcgac
     1021 cgtcgcgccg tccgggccgt gcaggcagtc cagcgcctcc tcgccgtcat gcgcctccag
     1081 cacggcgacg cccagcatct ccagcatgcg cgtcgcgagc atgcggttga cggcgttgtc
     1141 ctcgaccagc aggacgcggg ccgcgggcag cggggagggc ggggacgtga ggtcgttcat
     1201 gcgcgtgggg cctacactat cccacgccgt gcgcgtccgg ccggccgcgg tggccgggtg
     1261 gattgcggaa gaattcccgc aatgcgcctc cgcgtcgcgt ccatacccct acccgcatgc
     1321 ccgcgatccc cctgacgatc cgcccgttcc gcgccggcga cgagtccccg ctgcgcgcgg
     1381 tgtacgcctc ggccatccac gaggtcgcac ggcgcgacta cacgcaggcg cagctggacg
     1441 cctgggcgcc ggcgaacttc gacgccgaga actgggcgcg ccgcatccag ggcatcgcgc
     1501 ccttcgtcgc cgagggcgcg gacggcctcc tgctcggata tgccgacgtg cagcccgacg
     1561 gctacgtcga ccacttcttc gtcgccgggc acgcgggcgg gcagggcgtg ggcggcgcgc
     1621 tgatgcggcg gctgctcgag cgcgcgcgcg agctgggggt cgcgcagctc agctccgacg
     1681 tgagccggac cgcgcagccc ttcttcgcgc gcttcggctt
//
LOCUS       DASVLN010000723         6254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_36747, whole genome shotgun sequence.
ACCESSION   DASVLN010000723 DASVLN010000000
VERSION     DASVLN010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36747"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF453_17030"
     CDS             <1..193
                     /locus_tag="VF453_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX7689417.1"
                     /translation="RFIQSALREWAYGRAYKNSEERLSALPVWNHFYNWHRRHHGIGC
                     LPPMSRLLASRKNLLTLHI"
     gene            complement(234..1469)
                     /locus_tag="VF453_17035"
     CDS             complement(234..1469)
                     /locus_tag="VF453_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689418.1"
                     /translation="MPELIVCAALAFVVATYVLFSLKDGSWFNILTPQIFLGIPTLYI
                     AQLGRLYLGVDARASNAAWAFVYACYCLPLAIFAWTISHASIPIAMRIRTRRPLKIRA
                     SPWVLLGLGVLLYAPILIEFRASLLSPRSIYEQTRTGYGLYFFLSTMLAMLGFVLYLF
                     KTRSVARRLGFAAMCMLLSLSHGSKGLVIYLTLLWLLHWRYADGRRVPLRMAAALLGG
                     LSAVLVVLFYLFSSGVELGELAASMFGYSDYTANGLMIVDDDKPALWGRIFFEDNVYS
                     RIPRAIFPDKPKDFGSFLLAEIYFPESFELDQGVPAFGVGATFADFKWFSLPILCLAN
                     YLLGLLVRSLRNALARFRHPADFVVLAFLSGVPLLSVGVGFLLPEHMLLAVALTLALR
                     LRLRHRHAKTAAHSRAPAS"
     gene            complement(1548..2645)
                     /locus_tag="VF453_17040"
     CDS             complement(1548..2645)
                     /locus_tag="VF453_17040"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX7689419.1"
                     /translation="MDRTIGDATPEDDGVTLGDLFRPLSRHWKVVLATTVAAGALGYG
                     ASFLVKPRYLAPTVFLLPEQQTPGAAALASLGALSSLVGGAGAGSRSVSDEYIALLES
                     VTISDRIIQRFDLRKEWDVHYLVDARKRLRNVTSFYAGKKDGTMHVEVTDTDPVRAAA
                     MANQYVEELRTLNRTIAVTEAQQRRVFFERLLEETRDKLAAAQSALERSGFTGGALNS
                     EPRNAAESYARLKAELTSAQVKLQVLRATLADGSPQVVSQQQQVDSLAAQVAKLEGQG
                     QGSGTSGDYVNRYREFKYQETLFDLFARQYESARVDESREGALIQVIDPATPPEKKDW
                     PKRSIWSIVAALLGLVASWTWCGLRQRQPAA"
     gene            complement(2657..4711)
                     /locus_tag="VF453_17045"
     CDS             complement(2657..4711)
                     /locus_tag="VF453_17045"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX7689420.1"
                     /translation="MIRPLETSPRRPLAAAAIALALAACILPAHAQFDGTAGNDLTAV
                     GSRGNASNGTSTDDVDTAGTGNDIDNTGINGSEAGNGGGTTLRPSSSSTSARRTRTGS
                     SQPESRDRYGTRAPAYVPSEFELYVQRQAALSAPVRQASDVNAADAADRPAAVRIRRF
                     GANLVTDPLQTTALLDPLPTIPGDYLVRPGDEVDLAIWGTANADLRLIVDRAGRITVP
                     QVGAIQVAGLRNDELEPAISKRVALVFKNYHLSASIGQVRAIRVFVGGYAQRPGSVTL
                     GGLSSVLHAIMRAGGPSAAGSFRDIHLRRGGRDVAVFDLYDLLLKGDRAVDQYVQPDD
                     VIFIGPVGTQVAVLGSVNQQAIFETKPGETLADVLRMAGGFNAVADRRHVAVERLEDR
                     ATGHVTELALPQHAGDQLGTGDVVRVFSAISASVPTEFQNERVRVEGEVLHPGDYVLP
                     PGSKVSDALRLAGGMTRGAYPYATEFTRESVRQAQEANYQRALNDLETDMVKSRASER
                     ATSAEEINAKSNADTADQRLLARLRQIHPTGRVVLQVQPESTALPDLALEDGDVIKIP
                     PRNSSVGVFGSVFTTGSFVFEPGHSTGDYLALAGGPTRGADQDSMFMIRANGSVVSAR
                     QGSSFWHEGSAFRDAVVQPGDTLFVPERVDRTTFVQDAKDWTQILYQFGLGLAGIKSL
                     GL"
     gene            4915..>6254
                     /locus_tag="VF453_17050"
     CDS             4915..>6254
                     /locus_tag="VF453_17050"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7689421.1"
                     /translation="MTEELPADMSAPPAAEAGAATSPPAPAAQARFDTLPLDAKLLRA
                     VADQGYAFMTPIQAKAIPLVLQGRDVMGAAQTGTGKTAAFSIPLLQKMLRHETASVSP
                     ARHPVRALVLAPTRELADQVAQNIKAYAKHSHLRVAVVFGGIDMKPQTLELKGGVEVL
                     IATPGRLLDHIEAKNCVLNQVEYVVLDEADRMLDIGFLPDLQRILSYLPKAGRQTLLF
                     SATFSPEIKRLAASYLQDPVTVEVARANATASTVEQHFYSVTDDDKRAVVRQILKQRA
                     LSQAIVFVNSKLGAARLARSFERDGLRTSALHGDKSQDERLKALAAFKRAEVDVLVCT
                     DVAARGLDIADLPAVFNYDVPFNAEDYVHRIGRTGRAGASGLAVTLVTREDARNVSDI
                     EKLIHKKIELEPFEVEDDRPRRGPRRFDDEGGRRERSPSDRERERDAPGRVPPAR"
BASE COUNT         1027 a         2139 c         2172 g          916 t
ORIGIN      
        1 gcggttcatc cagtccgcct tgcgcgaatg ggcctacggg cgagcctaca agaactccga
       61 ggagcgcctt tcggccttgc ccgtctggaa ccacttctac aactggcacc gacgccacca
      121 cggcatcggt tgccttccgc ccatgtccag gctccttgcc tcacgcaaga acctgttgac
      181 acttcacatc taacgccgtc tgccgccgcc gccggccggc ggggtcgacg gcgctaggaa
      241 gctggcgccc gcgagtgcgc tgccgtcttc gcgtggcgat ggcgcaatcg caatcgcagg
      301 gcgagggtca gagccaccgc gagaagcatg tgctctggca gcaggaagcc gacaccgaca
      361 gacagcagtg gtacgccgct cagaaaggcg aggacgacga agtcggccgg atgacgaaag
      421 cgcgccagtg cgttgcgcag tgagcggacc agcaagccca aaagatagtt cgccaggcac
      481 aggatcggca gcgaaaacca cttgaaatcc gcgaaggtcg cgccgacgcc gaaggccggg
      541 acgccctggt ctagttcgaa actctcggga aaatagatct ccgcaagcag gaaagagccg
      601 aagtctttcg gcttgtccgg aaagatcgcg cgaggaattc gcgaatagac gttgtcttcg
      661 aagaagatcc ggccccagag tgcgggcttg tcgtcgtcga cgatcatcaa tccgttggcc
      721 gtgtagtcgg aatagccgaa catgctggca gccagttcgc caagttccac gccgctggaa
      781 aacaggtaga acagcaccac gagcacggcg gaaaggcctc cgagcagggc ggcggccatc
      841 cgcagcggca cccgccgacc gtccgcatag cgccagtgca gcaaccatag cagggtgagg
      901 tagatgacca gccccttgct gccatgggac agcgacagca acatgcacat cgccgcgaag
      961 cccaggcgcc gagcaacgct tctcgtcttg aacaggtaca ggacgaagcc gagcatcgcc
     1021 agcatcgtcg acaggaagaa gtagagaccg tatcccgtgc gggtctgttc gtaaatcgac
     1081 cgcgggctca gcagcgacgc gcggaattcg atgaggatgg gcgcgtacag cagcactccc
     1141 aaccccagca gcacccaggg gcttgcacga atcttcagcg ggcggcgggt gcggatgcgc
     1201 atcgcgatcg gaatcgacgc gtgcgagatc gtccaggcga agatcgctag cggaaggcag
     1261 tagcaggcgt agacaaaggc ccaggcagcg ttgctcgcgc gagcgtccac gcccaggtac
     1321 agccgcccca gctgggcgat gtacaacgtc gggataccca ggaagatttg cggcgtcagg
     1381 atgttgaacc agctcccgtc cttcagcgag aacaagacat aggtcgcgac gacgaacgcg
     1441 agtgccgcgc agacgatcag ctcgggcacg gcgacatgtc cctcgttgac cgcagccggg
     1501 gggccgagct catggatcgc acgcttggaa acgccgtcgc cccgacttca ggcagcgggc
     1561 tggcgttggc ggaggccgca ccatgtccaa ctcgccacga ggcccagcaa ggcggccacg
     1621 atggaccata tcgagcgctt gggccagtcc ttcttctcgg gcggcgtcgc cggatcgatc
     1681 acctggatca gcgcgccttc gcgactttcg tccacgcggg cgctctcgta ctggcgcgcg
     1741 aacaggtcga acagcgtctc ctggtacttg aactcgcgat agcggttcac gtagtcgccc
     1801 gaggttcccg agccttggcc ttggccttcc agcttcgcga cctgtgcggc cagcgaatcc
     1861 acctgctgct gctgcgagac cacttgcggc gagccgtcgg caagggtggc ccgcagcacc
     1921 tgcagcttga cttgcgcact ggtcagctcg gccttcaggc gggcgtagct ctccgcggcg
     1981 ttgcgcggct cggaattgag cgcgccacca gtgaagccgc tgcgctcgag cgccgactgc
     2041 gcggcggcga gcttgtcgcg cgtttcctcg agcaggcgct cgaagaacac gcgacgctgc
     2101 tgggcctccg tgacggcgat ggtgcggttg agcgtgcgca gctcctcgac gtactggttg
     2161 gccatcgcgg cggcgcggac gggatcggtg tcggtcacct ccacgtgcat cgtgccgtcc
     2221 ttcttgccgg cgtagaagct cgtcacgttg cgcaggcgct tgcgcgcgtc cacgaggtag
     2281 tggacgtccc actccttcct aaggtcgaag cgctggatga tgcggtcgct gatggtgacg
     2341 ctctcgagca gtgcaatgta ctcgtcgctc acgctgcgcg agcctgcacc agcgccgccg
     2401 acgaggctcg acagcgctcc aagcgaggcc agcgccgcgg cgccgggcgt ctgctgctcg
     2461 ggcagcagaa acacggtggg ggccagatag cgaggcttga ccaggaagct tgcgccgtag
     2521 cccagcgcgc cggccgccac cgtcgtcgca aggacgacct tccagtgccg ggacaatggt
     2581 ctaaagaggt cgcccagcgt cacgccgtcg tcttcaggcg tcgcgtcacc gatggttcga
     2641 tccatgctcg tcacgatcac aaccccagcg acttgatacc cgccagcccc aggccgaact
     2701 ggtacaggat ctgcgtccag tccttcgcgt cctgcacgaa ggtcgtgcgg tcgacgcgct
     2761 cgggcacgaa cagggtgtcg cccggctgga cgaccgcgtc gcggaacgcg ctgccctcgt
     2821 gccagaagct gctgccctgg cgtgcgctga cgaccgagcc gttggcgcgg atcatgaaca
     2881 tgctgtcctg gtcggcgccg cgcgtcgggc cgccggccag cgcgaggtag tcgcctgtcg
     2941 aatgcccggg ctcgaacacg aagctgccgg tcgtgaacac gctgccgaac acgcccaccg
     3001 aggagttgcg tggcgggatc ttgatgacgt cgccgtcctc cagcgcgagg tcgggcagcg
     3061 cagtcgactc cggctgcacc tgcagcacga cccggccggt cggatggatc tggcgcaggc
     3121 gcgcgagcag ccgctggtcg gcggtgtcgg cgttgctctt ggcgttgatc tcctcggcgg
     3181 aggtggcgcg ctcgctcgca cggctcttca ccatgtcggt ctcgagatcg ttcagcgcgc
     3241 gttggtagtt ggcctcctgc gcctgacgca cgctctcgcg cgtgaactcg gtggcatagg
     3301 gataggcgcc gcgcgtcatg ccgccggcca ggcgcagcgc gtcggagacc ttgctccccg
     3361 gaggcagcac atagtcgcca gggtgcagca cttcgccctc gacgcgcacg cgctcgttct
     3421 ggaactcggt gggcacgctc gcggagatcg cgctgaagac gcgcaccacg tcgccggtgc
     3481 ccagctggtc gccggcatgc tggggcagtg caagctccgt cacgtggccc gtcgcgcgat
     3541 cctcgaggcg ctccaccgcc acgtggcgac ggtcggccac ggcgttgaag ccgcccgcca
     3601 tgcgcagcac gtcggcgagc gtctcgcccg gcttggtctc gaagatcgcc tgctggttga
     3661 cgctgcccag caccgccacc tgcgtgccca ccgggccgat gaagatgacg tcgtccggct
     3721 gcacgtactg gtcgaccgcg cggtcgccct tgagcagcag gtcgtaaagg tcgaacaccg
     3781 cgacgtcgcg accgccccgg cgcaggtgga tgtcgcggaa gctgcccgcc gccgacggcc
     3841 cgccggcgcg catgatcgcg tgcaacaccg acgacagccc gcccagcgtg acgctgccgg
     3901 ggcgctgcgc gtagccgccg acgaagacgc ggatcgcgcg cacctggcca atggaggcgc
     3961 tcaggtggta gttcttgaag acgagggcga cgcgcttgct gatcgccggt tccagctcgt
     4021 cgttgcgcag gcccgccacc tggatcgcgc cgacctgggg cacggtgatg cggcctgcgc
     4081 ggtcgacgat caggcgcaag tcggcattgg cggtgcccca gatcgcgaga tcgacctcgt
     4141 cgcccgggcg caccagatag tcgccgggga tggtcggcag cggatcgagc agggcggtcg
     4201 tctgcagcgg atcggtgacc aggttggcgc cgaagcggcg gatccgcacc gccgccggcc
     4261 ggtcggccgc gtcggcggca ttgacgtcgc tcgcctgccg aaccggtgcc gacagcgcgg
     4321 cctggcgctg cacgtagagc tcgaactcgc tgggcacgta ggccggcgca cgcgtgccgt
     4381 agcgatcgcg cgactcgggc tgcgaactgc cggtgcgcgt ccggcgcgcg gacgtcgagg
     4441 acgacgacgg acgcagcgtc gtgccgccgc cgttgccggc ctccgaaccg ttgatgccgg
     4501 tgttgtcgat gtcgttgccg gtgccggcgg tgtcgacgtc gtccgtcgac gtgccgttcg
     4561 aggcgttgcc gcgactgccg acagcggtca ggtcgttgcc cgcggtgccg tcgaactgcg
     4621 catgggccgg caggatgcac gcggcgagcg cgagggcgat ggccgcggcg gcgagcggtc
     4681 ggcgaggcga ggtttccagg ggacggatca tcgtcggtgg caaggggaag cggtgggtcg
     4741 cggacgaccg gacccgggac gggcgggcat ggcgcggggc ccgcggagcg tggcgggacg
     4801 gcggcggacc gatcccggcg ggcgggcgcg aaaacggcgg attctagcgg tttcgccttg
     4861 cgcccgaccg ggcccgcgcg cgctggcggc cggcgcaggc gataatggcg gtccatgacc
     4921 gaagaactac ccgccgacat gtcggcacct cccgcggccg aggctggcgc cgcgacgtcg
     4981 cccccggccc ccgccgccca ggcgcgcttc gacaccctcc cgctggacgc caagctgctg
     5041 cgcgccgtcg ccgaccaggg ctacgccttc atgacgccca tccaggcgaa ggcgatcccg
     5101 ctggtgctgc agggacgtga cgtgatgggg gccgcgcaga cgggcaccgg caagacggcg
     5161 gcgttctcca tcccgctgct gcagaagatg ctgcgccacg agaccgccag cgtctcgccc
     5221 gcgcgccacc cggtgcgggc gctggtgctg gcgcccacgc gcgagctcgc cgaccaggtc
     5281 gcccagaaca tcaaggccta cgcgaagcac agccacctgc gcgtggcggt ggtcttcggc
     5341 ggcatcgaca tgaagccgca gacgctcgag ctgaagggcg gcgtcgaggt gctgatcgcg
     5401 acgccgggcc gcctgctcga ccacatcgag gccaagaact gcgtgctcaa ccaggtcgag
     5461 tacgtcgtcc tcgacgaggc cgaccgcatg ctcgacatcg gcttcctgcc cgacctgcag
     5521 cgcatcctga gctacctgcc caaggccggc cgccagacgc tgctgttctc ggcgacgttc
     5581 tcgcccgaga tcaagcgcct ggccgcgagc tacctgcagg atccggtgac ggtcgaggtc
     5641 gcgcgcgcca acgcgaccgc atcgacggtg gagcagcact tctacagcgt caccgacgat
     5701 gacaagcgcg cggtcgtgcg ccagatcctg aagcagcgcg cgctctcgca ggcgatcgtg
     5761 ttcgtcaact ccaagctcgg cgcggcgcgc ctggcgcgtt cgttcgagcg cgacggcctg
     5821 cgcacctccg cgctgcacgg cgacaagtcg caggacgagc gcctgaaggc gctggccgcg
     5881 ttcaagcgcg ccgaggtcga cgtgctggtc tgcaccgacg tcgccgctcg cggcctggac
     5941 atcgccgacc tgccggccgt cttcaactac gacgtgccgt tcaacgcgga ggactacgtc
     6001 caccgcatcg gccgcaccgg ccgcgcggga gcgtcggggc tcgcggtgac gctggtcacg
     6061 cgcgaggacg cgcgcaacgt cagcgacatc gagaagctga tccacaagaa gatcgagctc
     6121 gagcctttcg aggtcgagga cgatcgtccc cgccgcggcc cgcgccggtt cgacgacgag
     6181 ggcggccgtc gcgagcgcag cccctccgac cgcgagcgcg agcgcgacgc cccgggccgc
     6241 gtcccgccgg cgcg
//
LOCUS       DASVLN010000724         7612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_469295, whole genome shotgun sequence.
ACCESSION   DASVLN010000724 DASVLN010000000
VERSION     DASVLN010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469295"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1182
                     /locus_tag="VF453_17055"
     CDS             67..1182
                     /locus_tag="VF453_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX7689422.1"
                     /translation="MPAAALKGRGTAWAIPHRFAHDAREAVDDGWGALDQVATEERLA
                     PGTTVVEEHAGRILSTNDSPDIHFDAAINPYRGCEHGCIYCFARPTHSYLNLSPGLDF
                     ETRIIAKVNAAERLRETLASPRYEPTVLNIGAATDGYQPVERRLRITRAVVEVLAACR
                     HPFSIVTKSAGVERDLDLLGELGRAGLVAVYMSITTLDGELARIMEPRASAPHRRLRA
                     IETLSRAGVPVGVSASPMIPFVNEPELERILAAARDAGAVSAFSSVVRLPWEVNPLFQ
                     QWLEAHFPDRAARVMARVRDMRGGKDYDATFRTRMTGTGLWAQMLRDRFRLAAARLGY
                     GRERQPLDMSQFVRPVPDRPRGRAIARDEASGQGSLF"
     gene            complement(1228..3000)
                     /locus_tag="VF453_17060"
     CDS             complement(1228..3000)
                     /locus_tag="VF453_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689423.1"
                     /translation="MKSAQSEMKAATPAAFGGAGSRFDPDIVNCRALVIDANPTARSI
                     QAAQLRDLGVGNVVQCGRVQDARRQLEAREFDIVLCEQDFHGGNYSGQDLLNDLRRAQ
                     LLPFSTVFIMVTSEASYAAVAEAAESALDSYLLKPHTAHALAERIQQARQRKRILKDI
                     FIAIEDGEFELAAQLCVARFEARGAYWLYAARIGAELLLRVGRHDQARVMYEAVIATQ
                     ALPWARLGVARAQIEAGQGPAGRRTLESLIADQPGFADAYDVMGRVQVDQGQFAEALA
                     TYRTATQLTPGSIARLQRQGMLAFYLGENDEAKKALERATLLGISSRMFDFQTIVLLA
                     IARFTDRDSKGLQRCLDNINHALEKAPKSERLQRFASIVGIFNLMLQRQVAQVVAEVR
                     LLSKQLKEPGFDFEAGSNMVAMLAHLTAAELQLDAAEGWIDVLALRFCTTRSLSELLA
                     RAGAVHAPFAARIRDQHNAITALAEQAMSHSLAGEPRQAAKSLLSAGGRTMNAKLIDM
                     ARMVLQRHAAKIPDADDLLGMADELKQRYGSGGPQTAPLNGDAGRAAGGVAIRTRDKA
                     DGEADASAPDSGPPDATANGATPA"
     gene            complement(3068..4204)
                     /gene="dnaJ"
                     /locus_tag="VF453_17065"
     CDS             complement(3068..4204)
                     /gene="dnaJ"
                     /locus_tag="VF453_17065"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7689424.1"
                     /translation="MSKRDYYEVLGVAKNASDDDIKKAYRKLAMKYHPDRNQGDGAKS
                     AEEKFKEAKEAYEMLSDPQKKAAYDQYGHAGVDPNMGHGAGGEGFGGFAEAFGDIFGD
                     IFGGARRGGAGGGKQVYRGNDLSYAMEITLEEAAAGKDTQIRIPSWDTCETCKGSGAK
                     PGTSPKTCPTCSGAGTVHLRQGFFSIQQTCPNCHGTGKIIPEPCPSCSGQGRIKRQKT
                     LEVKIPAGIGEGMRIRSSGNGEPGPNGGPPGDLYIEIRIKQHDIFERDGDDLHCTVPV
                     PMTQAALGGSIEVPTLNGKVEIELPEGTQHGKTFRLRGKGIKGIRSSYPGDLYAHITL
                     ETPIKLTEHQRKLLRELDESLRKGGEKHSPNSKSWTDRVKDLFK"
     gene            complement(4412..6352)
                     /gene="dnaK"
                     /locus_tag="VF453_17070"
     CDS             complement(4412..6352)
                     /gene="dnaK"
                     /locus_tag="VF453_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7689425.1"
                     /translation="MAKIIGIDLGTTNSCVAIMEGNSTKVIENSEGARTTPSIIAYQE
                     DGEVLVGASAKRQAVTNPRNTLYAVKRLIGRKFTEKEVQKDIDLMPYKIAAADNGDAW
                     VEVRGKKIAPPQVSAEVLRKMKKTAEDYLGETVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKHGKGDRKIAVYDLGGGTFDISIIEIADVDGEMQFE
                     VLSTNGDTFLGGEDFDQRIIDYIISEFKKEQGVDLTKDVLALQRLKEAAEKAKIELSN
                     QTQTDVNLPYITADASGPKHLNIKLTRAKLESLVEDLIERTIAPCGTAIKDAGVKVSD
                     INDVILVGGMTRMPKVQDKVKEFFGKDPRKDVNPDEAVAVGAAIQGQVLSGDRKDVLL
                     LDVTPLSLGIETLGGVMTKMIKKNTTIPTKFSQVFSTADDNQPAVTIKVYQGERELAS
                     GNKSLGEFNLEGIPPSPRGVPQIEVTFDIDANGILHVSAKDKGTGKENKITIKANSGL
                     SEAEIEKMVNDAAANAAEDAKKVELVTARNQGDAMVHSVRKSLGEYGDKLDAGEKEKI
                     EAALKDVEEAIKGDDKAAIEAKTEALMAASQKLGEKMYADSQAAQAAAGAAAGADEAQ
                     ARPQPAADDNVVDADFKEVKKS"
     gene            complement(6491..6985)
                     /gene="grpE"
                     /locus_tag="VF453_17075"
     CDS             complement(6491..6985)
                     /gene="grpE"
                     /locus_tag="VF453_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7689426.1"
                     /translation="MQGESLDLPPLAGGEAARIAELEAKVAELNDAHLRAKAEAENAR
                     RRADEEVAKARKFAVEGFADSLLPVKDSLEAAIALPNATTEQVLEGVHATLRQLVSAL
                     ERNKVVQINPPAGTKFDPHQHQAISMVPADQEANTVVAVLQKGYLIAERVLRPALVTV
                     AAPK"
     gene            complement(7152..7547)
                     /locus_tag="VF453_17080"
     CDS             complement(7152..7547)
                     /locus_tag="VF453_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX7689427.1"
                     /translation="MRLDKWLWVARFFKTRALAAEEIEHGRVQVNGQVAKPARAVRVN
                     DRIEIRQPGTVRELLVRGLSAMRGPAPVARALYEETEASIAALQRAAEARRFGIEPAS
                     AQEQGRPTKRDRRDLAEWQRWSASIDDPD"
BASE COUNT          939 a         2598 c         2715 g         1360 t
ORIGIN      
        1 gggctgcgcg agctggcgcc cggggaggcg cccgagacgg cggccgatcc cggaacggcc
       61 gcccggctgc cggccgcggc gctcaagggg cgcggcacgg cctgggcgat cccccatcgc
      121 ttcgcgcacg atgcgcgcga ggcggtggac gacggctggg gcgccctgga tcaggtggcg
      181 accgaggagc gcctcgcgcc cggcaccacg gtcgtcgagg aacatgccgg gcgcatcctg
      241 agcacgaacg actcgcccga catccacttc gacgccgcga tcaaccccta ccgcggctgc
      301 gagcacggct gcatctactg cttcgcccgg ccgacgcaca gctacctgaa cctctcgccc
      361 gggctggact tcgagacccg catcatcgcc aaggtcaacg ccgcggagcg gctgagggag
      421 acgctcgcca gcccgcgcta cgaaccgacc gtgctgaaca tcggtgccgc caccgacggc
      481 taccaaccgg tcgagcggcg cctgcgcatc acgcgggccg tcgtggaggt gcttgccgca
      541 tgccggcatc cgttctccat cgtcaccaag tccgcgggcg tggagcgcga cctcgacctg
      601 ctcggcgagc tcgggcgcgc cggactggtg gcggtctaca tgtcgatcac gacgctggac
      661 ggcgagctgg cgcgcatcat ggagccgcgc gccagcgccc cgcatcgccg gctgcgggcg
      721 atcgagacgc tgtcgcgcgc gggcgtgccg gtgggcgtga gcgcgtcgcc gatgattccc
      781 ttcgtcaacg agccggagct ggagcgcatc ctggccgcgg cgcgcgatgc cggggctgtg
      841 agcgccttca gcagcgtggt gcggctgccg tgggaggtca acccgctctt ccagcagtgg
      901 ctcgaggcgc acttccccga ccgcgccgcg cgcgtgatgg cgcgcgtgcg cgacatgcgc
      961 ggcggcaagg actacgacgc caccttccgc acccgcatga ccggcaccgg gctgtgggcg
     1021 cagatgctgc gggaccgctt ccgcctcgcg gcggcgcgcc tcggctacgg acgcgagcga
     1081 cagccgctcg acatgagcca gttcgtgcgt ccggtgccgg atcgcccgcg cggccgcgcg
     1141 atcgcgcgcg acgaggcgag cgggcagggc agcctgttct gagcgatggc cgcggcgacg
     1201 gcgaaggcga tgccgcggtc ggggacgcta ggccggcgtc gctccgttgg ccgtcgcgtc
     1261 gggcgggccc gagtcgggcg ccgaggcatc cgcttctccg tccgccttgt ctcgggtgcg
     1321 gatcgcgacc ccgccggccg cgcggccggc gtccccgttc agcggcgcgg tctgcggccc
     1381 gccgctgccg tagcgctgct tgagctcgtc ggccatgccc aggaggtcgt cggcgtcggg
     1441 aatcttggcg gcgtggcgct gcagcaccat gcgcgccatg tcgatcagct tggcgttcat
     1501 cgtcctgccg ccggctgaca gcagcgactt cgccgcctgc cgcggttcgc ccgcgaggct
     1561 gtgcgacatc gcctgctccg cgagcgccgt gatggcgttg tgctggtcgc ggatgcgcgc
     1621 ggcgaagggc gcgtgcacgg cgccggcgcg ggcaagcagc tccgacaggc tgcgcgtcgt
     1681 gcagaagcgc agcgccagca cgtcgatcca gccctcggcg gcatcgagct gcagttcggc
     1741 cgcggtcagg tgcgcgagca tggcgaccat gttggagccg gcctcgaagt cgaagccggg
     1801 ctccttgagc tgcttggaca gcagacgcac ctccgcgacg acctgggcga cctggcgctg
     1861 cagcatcagg ttgaagatgc ccacgatcga cgcgaagcgc tgcaggcgct cgctcttggg
     1921 ggccttctcg agcgcgtggt tgatgttgtc gaggcagcgc tgcaggccct tggaatcgcg
     1981 gtcggtgaag cgcgcgatcg ccagcagcac gatggtctgg aagtcgaaca tgcgcgagct
     2041 gatcccgagc agggtcgcgc gctccagcgc cttcttggcc tcgtcgttct cgccgaggta
     2101 gaaggcgagc atgccctggc gttgcaggcg cgcgatggag cccggagtca gctgggtcgc
     2161 ggtgcggtag gtcgccagcg cctcggcgaa ctggccctgg tcgacctgca cgcggcccat
     2221 gacgtcgtag gcgtcggcga agccgggctg gtcggcgatc aggctctcca gcgtgcggcg
     2281 acccgccggc ccctggcccg cctcgatctg cgcccgcgcg acgcccagcc gtgcccaggg
     2341 cagcgcctgg gtcgcgatca cggcctcgta catgacccgc gcctggtcgt ggcggcccac
     2401 gcgcagcagc agctcggcgc cgatgcgcgc cgcgtagagc cagtacgcgc cgcgggcctc
     2461 gaatcgcgcc acgcagagct gggcggcgag ctcgaattcg ccgtcctcga tcgcgatgaa
     2521 gatgtccttg aggatgcgct tgcgctggcg cgcctgctgg atgcgctcgg ccagcgcgtg
     2581 cgcggtgtgc ggcttgagca ggtagctgtc cagcgccgac tcggcggcct cggccaccgc
     2641 cgcgtagctc gcctcggacg tgaccatgat gaacacggtc gagaagggca gcagctgggc
     2701 gcgccggagg tcgttgagca ggtcctggcc ggagtagttg ccgccgtgga agtcctgctc
     2761 gcacagcacg atgtcgaact cgcgcgcctc gagctggcga cgggcgtcct ggacgcggcc
     2821 gcactgcacg acgttgccca cgcccaggtc gcgcagttgc gcggcctgga tgctgcgggc
     2881 cgtcgggttc gcgtcgatga ccagggcgcg gcagttgacg atgtcggggt cgaagcgcga
     2941 tcccgcgccg ccgaatgcgg cgggcgtggc ggccttcatc tccgactggg cactcttcat
     3001 ggcaggacaa cccggctggc aggcgcccga agaggcgccc gtccacggtt atcggcccgc
     3061 gccggtccta cttgaagagg tccttgaccc ggtcggtcca gctcttggag ttgggcgagt
     3121 gcttctcgcc gcccttgcgc agcgactcgt cgagctcgcg cagcagcttg cgctggtgct
     3181 cggtgagctt gatcggggtc tcgagcgtga tgtgggcgta caggtcgccc gggtagctcg
     3241 accggatgcc cttgatgccc ttgccgcgca ggcggaaggt cttgccgtgc tgcgtgccct
     3301 cgggcagctc gatctcgacc ttgccgttga gcgtcggcac ttcgatcgag ccgccgagcg
     3361 cggcctgcgt catcggcacc ggcaccgtgc agtgcaggtc gtcgccgtcg cgctcgaaga
     3421 tgtcatgctg cttgatccgg atctcgatgt acaggtcgcc gggcggcccg ccgttcgggc
     3481 ccggctcgcc gttgcccgag gagcggatgc gcatcccctc gccgatgccg gcagggatct
     3541 tcacctccag cgtcttctgg cgcttgatcc ggccctggcc gctgcagctc gggcagggct
     3601 cagggatgat cttgccggtg ccgtggcagt tcgggcacgt ctgctggatc gagaagaagc
     3661 cctggcgcag gtgcaccgtg ccggcgcccg aacaggtcgg gcaggtcttg ggcgaggtgc
     3721 ccggcttggc gcccgagccc ttgcaggtct cgcaggtgtc ccagctcggg atgcggatct
     3781 gcgtgtcctt gccggcggcg gcctcctcca gcgtgatctc catcgcgtag ctgaggtcgt
     3841 tgccgcggta gacctgcttg ccgccgcccg cgccgccgcg gcgcgcgccc ccgaagatgt
     3901 cgccgaagat gtcgccgaag gcctccgcga agccgccgaa gccctccccg cccgcgccgt
     3961 gacccatgtt gggatcgacg ccagcgtggc cgtactggtc gtacgccgcc ttcttctgcg
     4021 gatccgagag catctcgtag gcctccttgg cctccttgaa cttctcctcg gccgacttcg
     4081 ccccgtcgcc ctggttgcgg tcggggtgat acttcatcgc gagcttgcga taggccttct
     4141 tgatgtcgtc gtcggacgcg ttcttggcga cgcccaggac ttcgtagtaa tcgcgtttgc
     4201 tcattgcagg tgaaccttgg ggatcgagcg cgggcggctt cggcggtggt cgggatccgc
     4261 gattctcgtc gatcgatcag ggccggcgcc gggtgccttc gaggcaaagg catccggcgc
     4321 ggcatttttc agcgaggaag gccggcggcc cgcgcggcgg ttctccggga aaccgcgcgc
     4381 aagacaccgg ccggtgccca gggcgggcgc ttcagctctt cttgacttcc ttgaagtcgg
     4441 cgtcgacgac gttgtcgtcg gccgccggct gcggacgggc ctgcgcctcg tcggcaccgg
     4501 ccgcggcgcc cgcggccgcc tgggccgcct gggagtcagc gtacatcttc tcgcccagct
     4561 tctggctcgc ggccatcagc gcctcagtct tggcctcgat cgcggccttg tcgtcgccct
     4621 tgatggcttc ctcgacatcc ttcagcgcgg cctcgatctt ctccttctcg ccggcgtcca
     4681 gcttgtcgcc gtactcgccc aggctcttgc gcacggagtg caccatcgcg tcgccctggt
     4741 tgcgggccgt gacgagctcg accttcttcg cgtcctcggc ggcgttggcg gcggcgtcgt
     4801 tcaccatctt ctcgatctcg gcctcggaca ggcccgagtt cgccttgatg gtgatcttgt
     4861 tctccttgcc ggtgcccttg tccttggcgc tcacgtgcag gatgccgttg gcgtcgatgt
     4921 cgaaggtgac ctcgatctgc ggcacgccgc gcggcgacgg cgggatgccc tcgaggttga
     4981 actcgccgag gctcttgttg cccgaggcca gctcgcgctc gccctggtag accttgatcg
     5041 tgacggccgg ctggttgtcg tcggcggtcg agaacacctg gctgaacttg gtcgggatcg
     5101 tggtgttctt cttgatcatc ttggtcatca cgccgccgag cgtctcgatg ccgagcgaca
     5161 gcggggtgac gtccagcagc agcacgtcct tgcggtcacc cgagagcacc tggccctgga
     5221 tggcggcgcc gacggcgacg gcctcgtccg ggttgacgtc cttgcgcggg tccttgccga
     5281 agaactcctt gaccttgtcc tgcaccttgg gcatgcgggt catgccgccg accaggatga
     5341 cgtcgttgat gtcgctcacc ttgacgcccg cgtccttgat cgcggtgccg cagggggcga
     5401 tcgtgcgctc gatcaggtcc tccaccagcg actcgagctt ggcgcgggtc agcttgatgt
     5461 tcaggtgctt cgggcccgac gcgtcggccg tgatgtaggg caggttgacg tcggtctgcg
     5521 tctggttgga cagctcgatc ttggccttct cggcggcttc cttcaggcgc tgcagggcca
     5581 gcacgtcctt ggtcaggtcg acgccctgtt ccttcttgaa ctccgagatg atgtagtcga
     5641 tgatgcgctg gtcgaagtcc tcgccgccca ggaacgtgtc gccgttggtg gacagcacct
     5701 cgaactgcat ctcgccgtcg acgtcggcga tctcgatgat cgagatgtcg aacgtgccgc
     5761 cgccgaggtc gtacaccgcg atcttgcggt cgcccttgcc gtgcttgtcc aggccgaacg
     5821 cgagcgccgc ggccgtcggc tcgttgatga tgcgcttgac gtccaggccg gcgatgcggc
     5881 ccgcgtcctt ggtcgcctgg cgctggctgt cgttgaagta cgcgggcacc gtgatcacgg
     5941 cctcggtgac cgtctcgccg aggtagtcct cggccgtctt cttcatcttg cgcagcactt
     6001 cggcggagac ctgcggcggc gcgatcttct tgccgcgcac ctcgacccac gcatcgccgt
     6061 tgtcggcggc ggcgatcttg tagggcatca ggtcgatgtc cttctggact tccttttccg
     6121 tgaacttgcg gccgatcagc cgcttgacgg catacagcgt gttgcgcggg ttcgtgaccg
     6181 cctggcgctt ggccgaggcg ccgaccagca cctcgccgtc ttcctggtag gcgatgatcg
     6241 acggcgtggt gcgggcacct tccgaattct cgatcacctt ggtggaattg ccttccatga
     6301 tggcgacgca cgagttcgtg gtcccgaggt cgatgccgat gatctttgcc atgttgagtt
     6361 ctcctggagc tgaaaacagc tgaatttctg ttgtttccga gatgcggatc ccggggctgg
     6421 tttcaagttg gaagcgccgg gactgcgcgc tttctcgcac ggatcggggc atcgcggccc
     6481 cggtccacgc tcacttcggg gccgcgacgg tcacgagcgc cgggcgcagc acgcgttcgg
     6541 cgatcaggta gcccttctgc aggacggcca cgacggtgtt ggcctcctgg tcggccggca
     6601 ccatcgagat cgcctggtgc tggtgcgggt cgaacttcgt gcccgcgggc gggttgatct
     6661 ggacgacctt gttgcgctcc agcgcggaga cgagctggcg cagcgtcgcg tgcacgccct
     6721 ccagcacctg ctcggtcgtc gcgttgggca gcgcgatcgc ggcttccagg ctgtccttga
     6781 cgggcagcag gctgtcggcg aagccctcga ccgcgaactt gcgcgccttg gcgacctcct
     6841 cgtcggcgcg tcggcgggca ttctcggctt cggccttcgc gcgcaggtgg gcgtcgttca
     6901 gctcggcgac cttcgcctcg agctcggcga tgcgggccgc ctcgccgccg gccagcggcg
     6961 gcaggtccag ggattcccct tgcaggggcg agttcaaggg cgcttgaggg ttctggtctt
     7021 cggcagtcat ggcgtcggtg aggtctcgat gtgggcgccg gcagcgccgg ttgcgccctg
     7081 aactggggac gtgcgaaacc gtttcaaggg ccggacggaa cttttttccg cgcacggcct
     7141 ctgcgcccgg atcaatccgg gtcgtcgatc gacgcgctcc agcgctgcca ctccgcgagg
     7201 tcgcggcggt cgcgcttggt cggccggccc tgctcctggg cggaggcggg ctcgatgccg
     7261 aaccggcgcg cctccgcggc gcgttggagc gcggcgatgc tcgcttcggt ctcctcgtag
     7321 agcgcgcggg cgacgggggc gggcccgcgc atcgcgctca ggccgcggac cagcaattca
     7381 cggaccgtgc cgggctggcg gatctcgatg cggtcgttga cgcgcacggc gcgcgcaggc
     7441 ttggcgacct ggccgttgac ctgcacgcgc ccgtgctcga tctcctcggc ggccagcgcc
     7501 cgcgtcttga agaagcgtgc gacccagagc cacttgtcca ggcgcatgtc ggcggcggct
     7561 tcgggcgccg aggagggggc ggggtgcttg gccatcgcgt cgaggggagt ca
//
LOCUS       DASVLN010000725        23857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_252583, whole genome shotgun sequence.
ACCESSION   DASVLN010000725 DASVLN010000000
VERSION     DASVLN010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 23857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23857
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252583"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF453_17085"
     CDS             complement(<1..318)
                     /locus_tag="VF453_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689428.1"
                     /translation="MPNRPAHLPPTGRTLGRAAAFRVSAAVVAHTLWTSAAPALSYPL
                     YAARWHLTPTVTTGMFAVYPIAVVLTLLLFGNLSDHVGRRLPMLAGVASSLAGVLLFA
                     LAPG"
     gene            complement(411..1016)
                     /locus_tag="VF453_17090"
     CDS             complement(411..1016)
                     /locus_tag="VF453_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX7689429.1"
                     /translation="MPKPSHREKLLQVGMQVVHQHGYLGASVRDIVQAAGVPQGSFTN
                     HFASKEAFALEVIDLYVEEARSAREGLRDDSHPPLQRLRAYIDATKNRLNRHGMRNGC
                     LLGNFTAEAAEQSEAIRARLLAHFGEGQAAIAYCLRAAVAAGEIAPSTDCDEVAAFVI
                     SSLQGANLMAKLVRSPEPVERFREILFTQVLPRIGPGTSAH"
     gene            complement(1090..1569)
                     /locus_tag="VF453_17095"
     CDS             complement(1090..1569)
                     /locus_tag="VF453_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7689430.1"
                     /translation="MPSDSPPLDAIDRKILGILQDSGRMSNVELAAAVQLSAPQCFRR
                     VRALEERGVIRGYRAAVDPLALGLGVAAYVSVNVEAGAFGRVREVEATLRDFPQVLEC
                     HIVSGDCDYLLKVVAGDLKALSHFLTDRLMQVPGVADVRSMICMEEIKPASALPCGG"
     gene            1744..2346
                     /locus_tag="VF453_17100"
     CDS             1744..2346
                     /locus_tag="VF453_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX7689431.1"
                     /translation="MEHTFLSAFILLLLVLDPFGSLPVFISILGVVPPERRTRVAVRE
                     VAIAFVVLLAFMVSGQGFLTLMRLSERSLEVAGGVILIIIAIRMIFGSGGGEIYGVDT
                     GRQPFIFPLAVPLLAGPSAMATVLLLASRQPEHILEWIGALTAALAVSGVVLLAADRI
                     RRVIGSQMVSAVEKLMGLVLTAVAVEMILAGLKRYFVGDL"
     gene            2394..3185
                     /locus_tag="VF453_17105"
     CDS             2394..3185
                     /locus_tag="VF453_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7689432.1"
                     /translation="MTRPTSTLSLTRRAAIALAAGAALLGASLADARSIEDIRKSGTL
                     VVATEGKFAPFNFVDHDKVAGFEIDVANAVAAKMGLKVEWKTMSFDGLLVGLAQDRWD
                     MVIASHGITPEREKAVTFANPHYCTGGVIISRDGSIKTAKDLTGKVVAVQTGTTYYDN
                     VKKLPVKDVKNFPQDNSARAALETGHVDAWVTDKFVAKASLAATPGTKLKTGDFLFTE
                     RIAAAVAKGNTGLANEVDKALAAIMADGTYAAISKKWFNEDIRCK"
     gene            3196..3993
                     /locus_tag="VF453_17110"
     CDS             3196..3993
                     /locus_tag="VF453_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7689433.1"
                     /translation="MSALKRLTSPWPAGWDRKRRSTATMAAAAVAMVVVLWALARVVS
                     FAPEPIGSNAQAFADGARVTVELTLVSGAAGIVLGIVAALGKLSRVPPVRWIASFYVW
                     VIRGTPLLIQVFFAYFALPQLLPFLKLEEFGWACVALALNVGAYNAEAIRSGLLAVPR
                     GQLEAARSLGMNRWFTFIDVTLPQAFKIALPPLVNNVVALLKDSSLASVIGLVELTNT
                     ADLLRAKTYLPIPSLVTSASIYLLLTTVMTQFSGAVERRFDIEDKLA"
     gene            4023..4763
                     /locus_tag="VF453_17115"
     CDS             4023..4763
                     /locus_tag="VF453_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7689434.1"
                     /translation="MPTAPLITVDNVCKSFGGVQVLKDVTTHFNAGEVAVIIGASGSG
                     KSTLLRTLNRLERHDSGRIVVDGIEVDDDHKRLDALRAEVGMVFQQFNLFPHLTVLEN
                     VALAPRRVRGLARAQAEARALELLERVGLKAHAHKHPFALSGGQQQRVAIARSLAMQP
                     RVMLFDEPTSALDPEMVKEVLDVMKELAASGMTMIVVTHEMGFAREVGDRILFFDRGR
                     IAQDAPPDRFFGAQEDERVRAFIGQVAR"
     gene            4843..7251
                     /locus_tag="VF453_17120"
     CDS             4843..7251
                     /locus_tag="VF453_17120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7689435.1"
                     /translation="MSRKIVIAGVASGLSCLAPLTALAQAASAAVSPSAAASAPADNI
                     AHVIITAQKRKEDIRDVPLSVSVLSGEQLQSQQINTVEDLTRNIPNISFSSQGGPGLG
                     TVEIRGVSSQAGQATVSVYLDDVSLTTRNLYSQGTAEPRFFDLANAEILRGPQGTLYG
                     ASSLGGTIKFVSNQPNLKEVEGYAQATVSGTKHGGLNWEVQGVSNIPLVKGTAALRLG
                     VEHGHESGYIDQVDPTTLQRIAKGIDGDDYTALKMMLKADMGHGWSILPALFAQQTDT
                     SDIATAYRDTQGGTPLPLYETSKTVREPGRDRLVVPSVTLSGDTSLGTFTGVLSGYMR
                     RFNRVQDGTAINDPSYAVENPDQYPGLVEALDALSSAVYLNNKIDQTSLEMRLASKDY
                     DPSQGSAFTWIGGAYVADTVTQVWDNEPIFGVNATFAKFGFNPASDPPLDGGFPDDFL
                     GDNSYYSARHYHDKQMSVFGEGTWHFSPTLSGTAGLRYLDASQHFARESSFFYAGANG
                     GVDPTQDPHWTATTPTFKLNWTVSPDISLYANASKGFRLGSANRGVPDTAKVRQDQAA
                     LGLSGPVPKSFDPDSLWSFEVGNKSDFLDHRLAFNASVFYLKWKNLQQDIILKNAGYD
                     YETNAGDATSYGIEAELRARVSEALTLNASLGYTHATFDQDNPAIGSVPVNPDDPEGP
                     RVLNVQKGDWVQGVPRGTAALGFDYHWAVSDAVGAFVRGSGQWTGKSRGSLDRSAADF
                     QRPSYFTADFSGGVNFERWEITAFVKNANNNHVAIQSPQVQKVDEAYYLRPRTIGMTV
                     NYAF"
     gene            7319..8110
                     /locus_tag="VF453_17125"
     CDS             7319..8110
                     /locus_tag="VF453_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7689436.1"
                     /translation="MPPVTSTLRRQALAVLLTCDPWEKAARARRLGLEELPVGAEEAI
                     AEPPGIPGRPARPALVPHTELKQRSVQTPQGRAALMHALAHIELNAIDLAADLAWRFA
                     GQPDEFYLDWARVAREEALHFGLLSERLGELGHAYGDFPAHNALWEMAEKTRHDLLAR
                     IALVPRTLEARGLDASPPIRRKLEAVGDERGTAILDVILRDEIGHVHVGNRWYARLCG
                     QRGLDPVATYEALAAEHGAPRPRGPFNLEARRAAGFSEEELARLG"
     gene            8261..8833
                     /locus_tag="VF453_17130"
     CDS             8261..8833
                     /locus_tag="VF453_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX7689437.1"
                     /translation="MALYRIGDAAPRVEEGAWVADSATVLGRVALERDASVWYGAVLR
                     GDNEWITIGERSNVQDGCVLHTDMGFPLTLGADVTVGHQVMLHGCTVGDNSLVGIQSV
                     VMNGARIGRNCIVGAGSLVTEGKEFPDNSLIVGSPAKAIRTLTDDQVARLKASAAHYV
                     HNAARHREEVVRIDEPSAAARARGADASAG"
     gene            8855..9805
                     /locus_tag="VF453_17135"
     CDS             8855..9805
                     /locus_tag="VF453_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEX7689438.1"
                     /translation="MSDLHKFIFDGLPVRGMLVRLTDSWQEVLARRAASGAWPRAVQD
                     MLGEMAAASVLMQANLKFNGALVLQVQGDGPVRLAVAEALTDLRFRVTAKVQGDVAAD
                     ARFEDLVNAGGHGRCAITLDPKDREPGQQPYQGVVSLVTPDGHSVGDVASMLEQYMRQ
                     SEQLDTRLVLAADGERACGLLVQRLPVEGVANLEGTAAGAVDEDAFTRIAHLAATLKA
                     DELLTLDAETILRRLFWEEPLRLFEPQHPSFACSCSRERVRSMLRGLGREEAEDILAE
                     RGDIEIACDFCGRQYRFDAVDVGEMFTAAADQPGAGSNVH"
     gene            complement(9886..10116)
                     /locus_tag="VF453_17140"
     CDS             complement(9886..10116)
                     /locus_tag="VF453_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689439.1"
                     /translation="MNRPILRHATKSGLVAVSAMLTAFCIAPVPAAASQVAGGSIGTV
                     VVTTPSSQRQTATHRTTTARKTAEKAASAAKH"
     gene            complement(10150..13377)
                     /locus_tag="VF453_17145"
     CDS             complement(10150..13377)
                     /locus_tag="VF453_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689440.1"
                     /translation="MFARATRVAALPATIALLGLPVAPLNAATPAAAPQVLLAITNSE
                     SMDGTTAGAIMVGSGSLGSSLSALQNSSSPMQYSVPAGFTPPVQAASGGMANYTAACL
                     TNSSYKCDNGPSRLNMTKAAIKSVLNSYGATINFGIYTYSTSGTSLYTTWVYQMSPTS
                     GSFGLTNTASANTVANPCYGYQSLPSSNAVYSDCNSISGQLGANALNTNPYMTISTSS
                     DDPQVNDVLYASGLAPVFLDYGQVSPANPYSHYSLSTYNNNPGSYSESYPYVTPSGNA
                     KTTTPTNAGFVPYSPQVMYIQRGFGYGGSQSATTGTATVAMGTDPTTSNGAQAFATAL
                     NPETNNASTGEIKAVAGQSAIAGLLAGAKTYMGNITKASCQSQYVVLMTDGLPTLDLS
                     GKTWPPLGTITGNAYNLTATFDSTGAFVSSNSQAVTDAMSSVTALANAGIKVYVIGLG
                     AGVDKNTNPIAYNLLQAMAIAGGTANFFPANDPSSLNNAFQTIVAQIYASSSIAAPLV
                     PKTVQSGSAYEYTATTTTPSAGHVTAYPVDSSGNPNGGPAWTTDNTMTITDRTTKLMG
                     GTGLTVLTSLTDAAFGLPTVKTTCVPDASTVASYTINPNASGLACSASTYLGSRASNV
                     LVGAFSMENQELYLGPPSSGLLTQKSGYVTWARAHASRPTAVLFTSSDGFLYSIDSTT
                     GALKWGWMNTYNLTQLQNYSTFQSTGPTNGDFTVVDSYDGSSWASYIVGSMNSGGERY
                     SLKLDDNGVPSSVIYDTYPGSSGNTPTGTTAGDTTAATGNAPLRQPPAIAYFGTAGTT
                     AYTIYVVTNGTTSTLWETNVTSGASTSAVLPFTVSSALFIDSASNQLWMGASNGQIWY
                     TTLSGTGAAVDVAALHQAGTTVNPATGAAVSNVQWVSYFKVNGNPYFYATNSGQITLF
                     GASATGWVPLWTATTAVNYTYTPPVPANGSAPAVPGKWVQVTSSANLLTTSAVISDMP
                     LVVGRLLVVPAFVAGSGCSLGLGYYDYFNLADGTFPTNALSDAGLGSITADQQIGTGA
                     AFTPSITITGNGYDLNRGSQNCTTLCGLDHGSNRLTNTSVSWRQH"
     gene            complement(13386..13979)
                     /locus_tag="VF453_17150"
     CDS             complement(13386..13979)
                     /locus_tag="VF453_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689441.1"
                     /translation="MTASMHPGHPSCARPARGDVLLIVLVFLLVFMLGLVVSMRGSIV
                     TTQLVGNNLQRQKDAQISDIALAQVEALMVANSQTTGQPLELSVGTGAAQPAWWRDVS
                     PGTAPPDASTYWGSCVGNTDATLRCAALTVTNNGSSIGPGYTVQAVVQPTGRTDTSNC
                     NIGPFLTANYYDIYLRIYEASSATTSITETVYKICTR"
     gene            complement(13976..15310)
                     /locus_tag="VF453_17155"
     CDS             complement(13976..15310)
                     /locus_tag="VF453_17155"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEX7689442.1"
                     /translation="MPSLAGSRRGGAAAGFTLVELLVALALSMILVGVMLKAQQSLAT
                     QTVHNADSETRDNEARTALDLINRTVTGAGFLLGGSPQACNEMFTYNSGAQSTSKYFV
                     HHPVDAVAATSGLYLPFASGLQLTYPGTSGTPTPSDVLAVTTSSDATGFDANNAPIVA
                     VSPNSAYTPMSTGKFPMQTTTGLTAGDTAILQVALTNRIACFRLPIASLSTASGSPDV
                     DDSAVNALMPSTFLNGYASSLTTAGYGTMTDAAVFQGQFVDITYVPPGMPVATTPRST
                     SAVTVVFYVDRDANYSFPVLKMATYSFKDDSVQTAPQIVAAGVVSLQVRFGVDPGKTG
                     AITAYHDAATVTTNNEWDTVRSTKVAIVSRSLTTESDMQGGSGNAAISSIPVCTTTPN
                     SPVKPCLESGFGFAAVTIPSSGSQQNHRYVVSTLEIANRNALWNCNGLICKK"
     gene            complement(15316..15828)
                     /locus_tag="VF453_17160"
     CDS             complement(15316..15828)
                     /locus_tag="VF453_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689443.1"
                     /translation="MKAGIATSHRRAARGQSGFTLLNVLVAALIFGLGMLGVAHSLGS
                     VTAAATQNENVTTLAPLSNTFWSAIQANSAVLTTTGWATTGGTTYNASTYTAAPAAVQ
                     PFLKQVVTLLPSGTAQIETLPDSSSGLACAVSTGCTVRLTISWAQVGSVRSSSSPTRS
                     QMFLYRYSQS"
     gene            complement(15825..16358)
                     /locus_tag="VF453_17165"
     CDS             complement(15825..16358)
                     /locus_tag="VF453_17165"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7689444.1"
                     /translation="MSTRLALQAGFTMIELMVTIAIIAIALALGVPYFNNATTGAKER
                     GVIAKLQQDFAWARTAAGVSDANSLNVSGASGLPVIRVTMFSTAGSCDWTMSVNGHTD
                     PAHSMTTAQLSSSTPGLSCPAGTGSSALSQPVTFSFSSQGTVDKSGLQTFHSSRGTDW
                     PLIFMLSGTVLINQGLS"
     gene            complement(16382..16858)
                     /locus_tag="VF453_17170"
     CDS             complement(16382..16858)
                     /locus_tag="VF453_17170"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEX7689445.1"
                     /translation="MLGVQPSQEPLPMALARPSRIRSTAARGFTLIELMITVAVIAIL
                     ARIALPAYTDYIRRGKLTEAFNQMTADALALGQYYQDNRTYVGATCPTSTTNFTYTCD
                     HLTSSGYLVTATGSTNSTTGFTYTLDASGTKVTVATSAGYPTSSSCWIASKSGTCF"
     gene            complement(16976..17647)
                     /locus_tag="VF453_17175"
     CDS             complement(16976..17647)
                     /locus_tag="VF453_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689446.1"
                     /translation="MAIVGAESTGKTTLAVALERALARDFGLHCVRVGEYLREWCDVN
                     DRTPRPDEQAPLAREQQRRIDAAAATPGVEVVLCDTTPLMTAVYSELLFDDCSLYAFA
                     AEAQWGVDLTLLTALDLPWVADGLQRDGPHVRAPVDAAVRARLLDWGLRWSLVSGVGG
                     ARTDAALDALRPLLRRRARHAGAGSGLFRRLMEASDQPQRPAWRCELCDDPACEHLGR
                     DVGEG"
     gene            complement(17670..18338)
                     /gene="pnuC"
                     /locus_tag="VF453_17180"
     CDS             complement(17670..18338)
                     /gene="pnuC"
                     /locus_tag="VF453_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEX7689447.1"
                     /translation="MLQALLAFLRPAFATAFTAWGTDVTWIEVVAFALSLWMVVCEMK
                     VRSLAWPLAIVTSLMYAALFADAKLYGEASLQLFFVAMSAWGWWQWRRGHGRDGGALM
                     VHRLARGPAVAVGMATLASWPALGWLLARTTDSPVPFCDALPTVVSIAATFLLGRKLI
                     ENWPAWLAVNVFSVGLFAYKGLWLTALLYALFAALSVAGWRVWRRLNGRSCATSSGRS
                     HAAA"
     gene            complement(18345..18620)
                     /locus_tag="VF453_17185"
     CDS             complement(18345..18620)
                     /locus_tag="VF453_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX7689448.1"
                     /translation="MRLVTIALLVLVGWVQAELWVGKSGMAHVMQLRGELEKLQAAND
                     VAKQHNDQLQAEVSDLKEGLEMVEEKARYELGMTKPDELLVVLTPGR"
     gene            complement(18652..19935)
                     /gene="eno"
                     /locus_tag="VF453_17190"
     CDS             complement(18652..19935)
                     /gene="eno"
                     /locus_tag="VF453_17190"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX7689449.1"
                     /translation="MSAIVDIVGREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     SREAIELRDGDKGRYLGKGVLKAVEHINTEISEAVLGLDASEQAFLDQTLIDLDGTDN
                     KGRLGANATLAVSMAVARAAAEESGLPLYRYFGGMGRMQMPVPMINVINGGAHANNNL
                     DIQEFMLLPIGAPSFREAVRYGAETFHALKKILADRGISTAVGDEGGFAPSVASHEAA
                     IQLLLEAIEKAGYKAGEQIAIGLDCAASEFYKDGKYHLEGEGLQLTAAEWTNMLATWV
                     DKYPIISIEDAMHEGDWEGWAHLTEALGKKVQLVGDDLFVTNTKILKEGIDKGIANSI
                     LIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSM
                     SRSDRIAKYNQLLRIEEDLGDIASYPGRAAFYNLR"
     gene            complement(20173..21834)
                     /locus_tag="VF453_17195"
     CDS             complement(20173..21834)
                     /locus_tag="VF453_17195"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX7689450.1"
                     /translation="MTKYVFVTGGVVSSLGKGIASASLAAILESRGLKVTLIKLDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFITTRMKRSNNFTAGQIYKSVLEKE
                     RRGDYLGKTVQVIPHVTNEIQEFVRRGASEAGVDVAICEVGGTVGDIESLPFLEAVRQ
                     MALKLGPTNSAFVHLTYVPFIAAAGELKTKPTQHTVQKMREIGIQPDVLLCRADREIP
                     VDEREKISLFTNVPMHGVISVWDAQTIYSVPRMLHEQGLDEQICMKLQLLTRPADLRR
                     WDLLVHEVNHPQREVTIAMAGKYTDLSDSYKSLNEALRHAGIQNHARVNIEYVDSEAL
                     TPENISQLGKFDAVLVPGGFGKRGVEGKILAAQYAREHAVPYLGICLGMQVATIEYAR
                     HKAGLANANSTEFDPNTPHPVIALIDEWEDRDGSIQKRDANSDLGGTMRLGAQSSDVE
                     PGTLASQIYGKVVTERHRHRYEANEHYLDRLKEAGLVISALTQREKLTEIVELPQEVH
                     PWFVGVQFHPEFKSTPWDGHPLFTSFIKAALAHHAAADATHKAAA"
     gene            complement(21955..22842)
                     /locus_tag="VF453_17200"
     CDS             complement(21955..22842)
                     /locus_tag="VF453_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7689451.1"
                     /translation="MKAPRIGTATDTIVFAHANGFPAGTYEPIFALWRARGWRVEAPE
                     RFSHDSRYPVTSNWPRLRDQLVDFIVARELAGAMVVGHSLGGLVGLLAACRRPDVVGG
                     LVMLDSPVVAGWRAHSLHMAKRMRLIERVGPGAISRRRRHEWPSREAVRAHFAAKAAF
                     ARWDPRMLDAYVHAGFDEAAAEPAHDPGDDGGAADTPRRIVLGIPREVETRIYNTLPH
                     HVPALLRKHPPRCPVGFIAGTRSTEMRQAGAAASRALAGERWRWIDGTHLYPMERPED
                     TALLVLELLASMRPPSARR"
     gene            complement(22849..23679)
                     /locus_tag="VF453_17205"
     CDS             complement(22849..23679)
                     /locus_tag="VF453_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HEX7689452.1"
                     /translation="MTYCVGIRLNAGLVFLSDSRTNAGLDQISTFRKMIVYEKPGDRF
                     MVLLSAGNLSISQSVREILQVEQLPDGDREPLTIWNATSMFDAARVLGSAVRRVYEND
                     APSLKNHGMDFVASFIFGGQIGNEAMRLFLVYSAGNFIEATRETCFFQIGESKYGKPI
                     LDRVLEPGTPLDEAAKCALVSMDSTLKSNLSVGLPLDLVVYEQGRLQSDQIVCIDEQN
                     PYFRMIRSSWGQRLRDVFESMEDPQWDPNAAGSHNALIVGEPRYDELKKIRNPDEKVV
                     "
BASE COUNT         3738 a         7913 c         8347 g         3859 t
ORIGIN      
        1 gcccggcgcg agcgcgaaca gcagcacgcc ggcgagcgag gacgccacgc ccgcgagcat
       61 cggcagccgc cggccgacgt ggtcggacag gttgccgaag agcagcagcg tcaggacgac
      121 cgcgatcggg tagaccgcga acatgccggt cgtgaccgtg ggcgtgaggt gccagcgcgc
      181 ggcatagagc gggtagctga gcgcgggcgc ggcgctggtc cacagcgtgt gggcgacgac
      241 ggccgccgag acgcggaacg cggcggcgcg gccgagcgtg cgacccgtgg gcggaaggtg
      301 ggcgggacgg ttgggcatgg cgggctcctg gatcgtcgaa taaatatgta cggacgtagt
      361 aaatgcggag acaaaaaaag cgcggcaagc cgcgcgcggc ctgccgcggg tcagtgcgcg
      421 ctcgttcccg ggccgatgcg cggcagcacc tgcgtgaaca ggatctccct gaaccgctcg
      481 accggttcgg ggctgcgcac cagcttggcc atcaggttgg cgccctgcag cgaggagatg
      541 acgaaggccg cgacctcgtc gcagtcggtc gagggggcga tctcgcccgc ggccaccgcg
      601 gcgcgcaggc agtaggcgat cgccgcctgg ccttcgccga agtgggccag caggcgcgcg
      661 cggatcgcct cgctctgctc ggccgcctcg gccgtgaagt tgcccagcag gcagccgttg
      721 cgcatgccgt ggcggttcag ccggttcttc gtggcgtcga tgtaggcgcg caggcgctgc
      781 agcggcgggt ggctgtcgtc gcgcaggccc tcgcgcgccg agcgggcctc ctcgacgtag
      841 aggtcgatca cctcgagcgc gaacgcctcc ttcgacgcga aatggttcgt gaacgagccc
      901 tgcggcaccc cggcggcctg cacgatgtcg cgcacgctgg caccgagata gccgtgctgg
      961 tggacgacct gcatgccgac ctgcaggagc ttctcgcgat gggagggctt gggcatgatc
     1021 ggaacaatac agtcgtacgt atttcgtgtc aagccgccca tcgaccggca ccgcccggcg
     1081 cggcggcgat cagcctccgc agggcagggc ggaggcgggc ttgatctctt ccatgcagat
     1141 catggagcgc acgtcggcga cgcccggcac ctgcatcagg cgatcggtga ggaagtgcga
     1201 gagcgccttc aggtcgccgg caacgacctt gagcaggtag tcgcagtcgc ccgagacgat
     1261 gtggcattcg agcacctggg ggaagtcgcg cagcgtcgcc tcgacctcgc gcacgcgccc
     1321 gaaggcgccg gcttccacgt tcacgctgac gtaggcggcg actcccaggc ccagcgccag
     1381 cgggtcgacc gcggcccggt agccgcggat cacgccgcgc tcttcgagcg cccgcacgcg
     1441 ccggaagcac tgcggcgccg agagctgcac cgccgccgcg agctccacgt tggacatccg
     1501 tcccgagtcc tggaggattc caagaatctt gcggtcgatc gcgtcgaggg gcggtgaatc
     1561 gctgggcacg ggtcattcta tgaaaggaac tgctccaaac tggcggattc gacgcaatga
     1621 ttcgaaatca aattcctctc caccacgaat aaagtcgagc gcttcgtgac aacgcgacga
     1681 caatgcccgg cgccggccat ccggccggac cttcgcgccc tgcggacacc ctccgcgccg
     1741 tccatggaac acaccttcct ctccgcgttc atcctgctgc tgctggtgct cgacccgttc
     1801 ggcagcctgc ccgtcttcat ctccatcctg ggcgtggtgc cgccggagcg gcgcacgcgt
     1861 gtggcggtgc gcgaggtcgc gatcgccttc gtcgtgctgc tcgcgttcat ggtgtcgggg
     1921 cagggcttcc tgacgctcat gcggctgtcc gagcggtcgc tggaggtcgc cggcggcgtg
     1981 atcctgatca tcatcgcgat ccgcatgatc ttcggcagcg gcggcggcga gatctacggc
     2041 gtcgacaccg gtcgccagcc cttcatcttc ccgctcgcgg tgccgctgtt ggccgggccg
     2101 tcggccatgg ccaccgtgct gctgctcgcc tcgcgccagc ccgagcacat cctcgaatgg
     2161 atcggcgcgc tcaccgcggc gctggcggtc agcggcgtcg tgctgctggc ggccgaccgc
     2221 atccgccgcg tgatcggctc gcagatggtc tcggccgtcg agaagctgat gggcctcgtg
     2281 ctcacggcgg tcgcggtgga gatgatcctc gccggcctga agcgctactt cgtcggcgac
     2341 ctctgaacgc gcacctcttt cccgtctttg atttgatacg caggagctcc ccgatgaccc
     2401 gtcccacctc gaccctgtcc ctcacgcgcc gcgccgcgat cgcgctggcc gccggcgccg
     2461 cgctgctggg cgcgtccctc gccgatgccc gcagcatcga ggacatccgc aagagcggga
     2521 cgctggtcgt cgccaccgag ggcaagttcg cgccgttcaa cttcgtcgac cacgacaagg
     2581 tcgccggctt cgagatcgac gtcgccaacg ccgtcgccgc caagatgggc ctgaaggtcg
     2641 agtggaagac gatgagcttc gacggcctgc tcgtgggcct cgcgcaggat cgctgggaca
     2701 tggtgatcgc ctcgcacggc atcacgccgg agcgcgagaa ggccgtgacc ttcgccaacc
     2761 cgcactactg cacgggcggc gtcatcatct cgcgcgacgg ctccatcaag acggccaagg
     2821 acctgaccgg caaggtcgtc gcggtgcaga ccggcacgac ctactacgac aacgtgaaga
     2881 agcttccggt caaggacgtc aagaacttcc cgcaggacaa ctcggcgcgc gcggcgctcg
     2941 agaccggcca cgtcgacgcc tgggtgaccg acaagttcgt ggccaaggcc tcgctcgcgg
     3001 cgacgcccgg caccaagctg aagaccggcg acttcctctt caccgagcgc atcgccgccg
     3061 cggtcgccaa gggcaacacc gggctcgcca acgaggtgga caaggcgctg gccgcgatca
     3121 tggccgacgg cacgtacgcg gccatctcga agaagtggtt caacgaagac atccgctgca
     3181 agtgacggcc ccgcgatgag cgccctgaaa cggttgacct cgccctggcc cgcgggctgg
     3241 gatcgcaagc gccgcagcac ggcgacgatg gccgccgccg cggtcgcgat ggtggtggtg
     3301 ctctgggcgc tggcgcgcgt cgtctcgttc gcgcccgagc cgatcggcag caacgcgcag
     3361 gccttcgccg acggcgcccg cgtgacggtg gagctgacgc tggtctcggg cgcggcgggc
     3421 atcgtgctgg gcatcgtcgc cgcgctgggc aagctctcgc gcgtgccgcc ggtgcgctgg
     3481 atcgcgtcgt tctacgtctg ggtgatccgc ggcacgccgc tgctgatcca ggtcttcttc
     3541 gcctacttcg cgctgccgca gctgctgccg ttcctcaagc tggaggagtt cggctgggcc
     3601 tgtgtcgcgc tggcgctcaa cgtgggtgcc tacaacgcgg aggcgatccg ctcgggcctg
     3661 ctcgcggtgc ccagggggca gctcgaggcg gcacgctcgc tgggcatgaa ccgctggttc
     3721 acgttcatcg acgtcacgct gccgcaggcg ttcaagatcg cgctgccgcc gctggtcaac
     3781 aacgtcgtcg cgctgctgaa ggactcgtcg ctcgcgtccg tcatcgggct cgtcgagctc
     3841 accaacacgg ccgacctgct gcgggcgaag acctacctgc cgatcccgtc gctcgtgacc
     3901 tcggcgtcga tctacctgct gctcacgacg gtcatgaccc agttctccgg cgcggtggag
     3961 cgtcgcttcg acatcgagga caagctggca tgagtggcac gagtcttgcg aaagatgcgg
     4021 ccatgcccac cgcgccgctc atcaccgtcg acaacgtctg caagtccttc ggcggcgtgc
     4081 aggtgctcaa ggacgtcacg acgcacttca acgccggaga ggtggcggtg atcatcggcg
     4141 cctccggctc gggcaagagc acgctgctgc gcaccctcaa ccggctcgaa cgccacgaca
     4201 gcggccgcat cgtcgtcgac ggcatcgagg tcgacgacga ccacaagcgg ctcgacgcgc
     4261 tgcgcgcgga ggtcggcatg gtgttccagc agttcaacct gttcccgcac ctgaccgtgc
     4321 tcgagaacgt ggcgctggcg ccgcgccgcg tgcgcgggct cgcgcgcgcg caggcggagg
     4381 cgcgcgcgct ggagctgctc gagcgcgtcg gcctgaaggc gcatgcgcac aagcatccgt
     4441 tcgcgctgtc cggcggccag cagcagcgcg tggcgatcgc gcgcagcctc gcgatgcagc
     4501 cgcgcgtgat gctgttcgac gagccgacct ccgcgctcga cccggagatg gtcaaggaag
     4561 tgctcgacgt gatgaaggag ctcgcggcaa gcggcatgac gatgatcgtc gtgacccacg
     4621 agatgggctt cgcgcgcgag gtcggcgacc gcatcctctt cttcgaccgc ggccgcatcg
     4681 cgcaagacgc gccgcccgac cggttcttcg gcgcccagga ggacgaacgc gtgcgcgcgt
     4741 tcatcggcca ggtcgcccgc tgaaggcgca ttcgtccgcc acgacgacga agcgcgagtc
     4801 gactccccac ccaccaccca cgtcatcccc gaggagggac gcatgtccag aaagatcgtg
     4861 atcgccggcg tcgcatccgg cttgtcctgt ctcgcgcccc tgaccgcgct cgcgcaggcg
     4921 gcgtccgcgg cggtctcgcc ttccgcggcc gcgagcgctc cggccgataa catcgcgcac
     4981 gtgatcatca cggcgcagaa gcgcaaggag gacatccgcg acgtgccgct gagcgtgtcg
     5041 gtgctctcgg gcgagcagct gcagtcgcag cagatcaaca cggtcgagga cctgacgcgc
     5101 aacatcccca acatctcgtt ctcgtcccag ggcggcccgg ggctgggcac cgtggagatc
     5161 cgcggcgtga gctcgcaggc gggccaggcg acggtcagcg tctacctcga cgacgtctcg
     5221 ctcacgacgc gcaacctcta cagccagggc acggccgaac cgcgcttctt cgacctcgcc
     5281 aacgccgaga tcctgcgcgg cccgcagggc acgctctacg gcgcgagctc gctgggcggc
     5341 acgatcaagt tcgtcagcaa ccagccgaac ctgaaggagg tcgagggata cgcgcaggcg
     5401 accgtctcgg gcacgaagca cggtggtctg aactgggaag tgcagggcgt ctccaacatc
     5461 ccgctggtca agggcaccgc ggcccttcgg ctgggcgtcg agcacggcca cgagagcggc
     5521 tacatcgacc aggtcgaccc gacgacgctg cagcgcatcg ccaagggcat cgacggcgac
     5581 gactacacgg ccctcaagat gatgctgaag gccgacatgg ggcacggctg gagcatcctg
     5641 cctgcgctgt tcgcccagca gaccgacacc agcgacatcg ccacggccta ccgcgacacg
     5701 cagggcggca ccccgctgcc gctgtacgag accagcaaga ccgtgcgcga gcccgggcgc
     5761 gaccgcctgg tggtgccgag cgtcacgctc tccggcgaca cctcgctcgg cacgttcacc
     5821 ggcgtgctga gcggctacat gcgccgcttc aatcgcgtcc aggacggcac cgcgatcaac
     5881 gacccgagct acgcggtcga gaaccccgac cagtatcccg gcctcgtcga ggccctcgac
     5941 gcgctgtcct cggcggtcta cctgaacaac aagatcgacc agacctcgct cgagatgcgc
     6001 ctcgcgtcga aggactacga cccgagccag ggcagcgcct tcacctggat cggcggcgcg
     6061 tacgtcgccg acacggtgac gcaggtctgg gacaacgagc cgatcttcgg cgtgaacgcg
     6121 acgttcgcga agttcggctt caacccggcg agcgatccgc cgctggatgg cggcttcccc
     6181 gacgacttcc tgggcgacaa ctcctactac agcgcacgcc actaccacga caagcagatg
     6241 tccgtgttcg gcgaaggcac ctggcacttc tcgccgacgc tgtcgggcac cgcgggcctg
     6301 cgctacctcg acgcgtcgca gcacttcgcc cgcgagtcgt cgttcttcta tgcgggcgcc
     6361 aacggcggcg tcgatccgac gcaggatccg cactggacgg cgaccacgcc gacgttcaag
     6421 ctgaactgga cggtctcgcc cgacatctcg ctctacgcga acgcctccaa gggctttcgc
     6481 ctcggcagtg cgaaccgcgg cgtgcccgac acggcgaagg tccggcagga ccaggcggct
     6541 ctcggcctgt cggggcccgt gccgaagtcc ttcgaccccg acagcctctg gagcttcgag
     6601 gtcggcaaca agtccgactt cctcgaccac cgcctcgcgt tcaacgcctc ggtcttctac
     6661 ctcaagtgga agaacctcca gcaggacatc atcctgaaga atgccggcta cgactacgag
     6721 acgaacgccg gcgatgcgac cagctacggc atcgaggccg agctgcgcgc gcgggtgtcc
     6781 gaggcgctga ccctcaacgc ctccctgggc tacacgcacg cgaccttcga ccaggacaac
     6841 cccgcgatcg gttcggtgcc ggtcaatccg gacgatccgg agggcccgcg cgtgctgaac
     6901 gtgcagaagg gcgactgggt gcagggcgtg ccgcgcggca ccgcggcgct gggcttcgac
     6961 taccactggg ccgtcagcga tgcggtcggc gcgttcgtgc gaggcagcgg ccagtggacg
     7021 ggcaagtcgc gcggctcgct cgatcgctcg gcggccgact tccagcgtcc gtcctacttc
     7081 acggcggact tcagcggcgg cgtcaacttc gagcgctggg agatcacggc cttcgtcaag
     7141 aacgccaaca acaaccatgt cgcgatccag tcgccgcagg tgcagaaggt ggatgaggcc
     7201 tactacctgc ggccgcgcac gatcgggatg acggtcaatt acgcgttctg acccggacga
     7261 cggccaggag gacggcacgt cggcagcggc gccgaggcgg acgcgtccgc cgcgtaacat
     7321 gccgcccgtg acttcgacgc tccgccgcca ggccctggcc gtcctgctga cctgcgaccc
     7381 ttgggagaag gcggcccgcg cgcgccgtct cggccttgag gagctgcccg tcggggcgga
     7441 ggaggcgatc gccgagccgc cgggcatccc cggccggccc gcgcggccgg cgctggtgcc
     7501 gcacaccgaa ctgaagcagc gctcggtgca gacgccgcag ggccgcgccg cgctgatgca
     7561 cgcgctcgcg cacatcgagc tcaacgcgat cgacctggcc gccgacctcg cgtggcgctt
     7621 cgccgggcag cccgacgagt tctacctcga ctgggcgcgc gtcgcgcgcg aggaggcgct
     7681 gcacttcggg ctgctgagcg agcggctggg ggagctcggc cacgcgtacg gcgacttccc
     7741 cgcgcacaac gcgctgtggg agatggccga gaagacgcgc cacgacctgc ttgcccgcat
     7801 cgcgctcgtg ccgcgcacgc tggaagcccg cggcctggac gcctcgccgc cgatccggcg
     7861 caagctcgag gccgtgggcg acgagcgcgg cacggccatc ctcgacgtga tcctgcgcga
     7921 cgagatcggc cacgtccacg tcggcaaccg ctggtacgcg cgcttgtgcg ggcagcgcgg
     7981 cctcgatccc gtcgcgacct acgaggcgct cgccgccgag cacggcgcgc cgcgtccgcg
     8041 cgggcctttc aacctcgagg ccaggcgcgc cgctggcttc agcgaggaag agctggccag
     8101 gctgggttga agaagcggcg gaacgcccca cgtgggacgg ctggcgcgaa aggcccgcgc
     8161 gtcgcgccgg ccgaccccgt cttctaaaat cccgcaccgc cttcggccgc cgccgccgcg
     8221 cggcgcctgc gccttcgcgc gcccaccttc ggagactgcc atggccctgt accgcatcgg
     8281 cgatgccgcc ccccgtgtcg aggagggcgc gtgggtcgcc gactccgcga ccgtgctcgg
     8341 ccgcgtggcg ctcgagcgcg acgccagcgt ctggtacggc gccgtgctgc gcggcgacaa
     8401 cgagtggatc accatcggcg agcgcagcaa cgtccaggac ggctgcgtgc tgcacaccga
     8461 catgggcttc ccgctgacgc tgggcgcgga cgtgacggtc ggccaccagg tgatgctgca
     8521 cggctgcacg gtgggcgaca actcgctcgt gggcatccag tcggtggtca tgaacggcgc
     8581 gcgcatcggc cgcaactgca tcgtcggcgc gggctccctg gtgaccgagg gcaaggagtt
     8641 cccggacaac tcgctgatcg tcggcagccc cgccaaggcg atccgcacgc tcaccgacga
     8701 ccaggtcgcg cgcctgaagg cgagcgcggc gcactacgtg cacaacgccg cgcgccaccg
     8761 cgaggaggtg gtgcgcatcg acgaaccctc ggccgccgcc cgcgcgcgcg gcgccgacgc
     8821 cagcgccggc tgacggcgcc ccggacgaga catcatgagc gatctccaca agttcatctt
     8881 cgacggcctg ccggtgcgcg gcatgctggt gcgcctgacc gacagctggc aggaggtgct
     8941 ggcccgccgc gccgcgagcg gcgcctggcc gcgggccgtg caggacatgc tcggcgagat
     9001 ggccgccgcg tcggtgctga tgcaggccaa cctcaagttc aacggcgcgc tggtgctgca
     9061 ggtgcagggc gacggcccgg tgcgcctggc ggtggccgag gcgctgaccg acctgcgatt
     9121 ccgggtcacc gccaaggtgc agggcgacgt cgccgcggac gcgcgcttcg aggatctcgt
     9181 caacgccggc ggccacggcc gctgcgcgat cacgctggat ccgaaggacc gcgagcccgg
     9241 ccagcagcct taccagggcg tggtgtcgct cgtgacgccg gacggccatt ccgtgggcga
     9301 cgtggcgagc atgctggagc agtacatgcg ccagtcggag cagctcgaca cccggctcgt
     9361 gctcgccgcc gacggcgaac gcgcctgcgg gctgctggtc cagcgcctgc cggtcgaggg
     9421 cgtcgccaac ctcgagggca cggccgcggg cgcggtcgac gaggacgcct tcacccgcat
     9481 cgcccacctc gcggcgacgc tgaaggccga cgaactgctg accctggacg ccgagaccat
     9541 cctgcggcgc ctgttctggg aggaaccgct gcgcctgttc gagccgcagc atccgtcctt
     9601 cgcctgcagc tgctcgcgcg agcgcgtgcg ctcgatgctg cgcggcctcg gccgcgagga
     9661 ggccgaggac atcctcgccg agcgcggcga catcgagatc gcctgcgact tctgcggccg
     9721 ccagtaccgg ttcgacgcgg tcgacgtggg cgagatgttc acggccgccg ccgaccagcc
     9781 cggcgccggc tcgaacgtgc actgacgcgt ctgcgcgacg cgcccaagcg aaaagtcggc
     9841 cgtgatcagg ccgcccgacc tacaccgtgg cgccgccgct catggtcagt gcttggcggc
     9901 cgaggccgcc ttctcggcgg ttttccgcgc cgtggttgtg cggtgcgtgg ccgtctggcg
     9961 ctgactgctc ggagtggtga ccacgaccgt gccgatactg cccccggcaa cctgagaggc
    10021 ggccgcaggg acgggcgcga tgcagaacgc tgtcagcata gccgacacgg ccacgaggcc
    10081 cgacttggtg gcgtgacgaa gaatggggcg gttcatggcg atttcctcgg taaagtcatc
    10141 ggcgccggat cagtgttgac gccagctgac ggaggtgttg gtcagccgat tgctgccatg
    10201 gtcgaggccg cacagcgtgg tgcagttctg tgagccgcga ttgaggtcat agccattgcc
    10261 ggtgatggtg atactgggcg tgaatgctgc gccggtgccg atctgttggt ctgctgtgat
    10321 gctacccagg cccgcatcgc tcagagcgtt cgtcgggaag gtgccatccg ccagattgaa
    10381 atagtcgtag tagcccaggc ccagcgaaca acccgacccc gcgacgaagg ctggcaccac
    10441 cagcagccga cccacgacaa gtggcatgtc cgagatcacg gcgctggtag taagcaggtt
    10501 cgcagaactg gtcacctgca cccacttgcc cgggactgcc ggcgcggaac cgttggccgg
    10561 tactggcggc gtataggtgt aattgacagc ggtggtggcc gtccacaacg gcacccagcc
    10621 cgtcgcgctg gcgccgaaga gggtgatttg gcctgagttg gtggcgtaga agtacgggtt
    10681 gccgttcacc ttgaagtagc tcacccactg gacattgctg acggccgcgc ccgtggctgg
    10741 gttgactgtc gtgccggcct gatgcagggc ggcgacatcg accgccgcgc cagtgccgct
    10801 caacgtcgtg taccagatct gaccgttact cgcgcccatc cagagctggt tgctggcgct
    10861 gtcgataaac aatgcgctgc tgacggtgaa cgggaggacg gccgaagtcg acgcgccgct
    10921 cgtcacgttg gtttcccaca aggtgcttgt ggtgccattc gtgacaacgt agatcgtgta
    10981 cgcggtggtg ccggcggtgc cgaaatacgc aatggccggt ggttgacgaa ggggcgcatt
    11041 gccggtggcg gccgtcgtgt cgccagcggt cgtgcccgtg ggggtattgc cgctgctgcc
    11101 ggggtaggtg tcgtagatca ccgagcttgg tacgccattg tcgtcgagct tgagcgaata
    11161 gcgctcacca ccggagttca tgctgccgac gatgtagctc gcccaggacg atccgtcata
    11221 ggaatccacc accgtgaagt cgccattggt cgggccagtc gactggaagg tcgaatagtt
    11281 ctggagctgg gtcaggttgt atgtgttcat ccagccccac ttcagcgcac cggtggtcga
    11341 gtcgatcgag tacaggaagc cgtcggagct cgtgaacagc actgccgtcg ggcgcgacgc
    11401 gtgcgcccgt gcccatgtga cgtagcctga tttctgggtc agcaggccgc tgctgggcgg
    11461 accgaggtag agctcctggt tctccatgct gaaggcgccc accagcacgt tggaggcgcg
    11521 gctgcccagg taagtagacg cgctgcaggc gaggccggat gcattgggat tgatcgtgta
    11581 actggcgacc gtgctggcgt cggggacgca ggtcgtcttc acggtgggca gtccgaaggc
    11641 cgcgtccgtc agtgatgtca gcacagtgag gccagtgccg cccatcagtt tggttgtccg
    11701 gtcagtgatg gtcatcgtgt tgtccgtcgt ccaggccggt cccccattag ggttgcccga
    11761 cgagtccacc ggatatgccg tgacgtgacc agccgaaggc gtcgtggtcg ttgcggtgta
    11821 ctcgtacgcg gagccgctct gcacggtctt gggcaccagc ggcgcggcga tcgagctcga
    11881 ggcgtagatc tgcgcgacga tggtctggaa cgcgttgttg agggaggacg ggtcgttggc
    11941 ggggaagaaa ttcgccgtgc cgcccgcgat cgccatggcc tgcagcaagt tgtaggcgat
    12001 cgggttggta ttcttgtcga cgcccgcgcc caggccgatc acgtagacct tgatgccggc
    12061 gttggcgagg gccgtgaccg agctcattgc gtcggtcaca gcctgtgagt tggacgacac
    12121 gaacgcgccg gtgctgtcga acgtcgcggt taggttgtac gcgttgccgg tgatcgtgcc
    12181 aagcggaggc caggtcttac cggacaggtc gagcgtcggc agcccgtccg tcatcagcac
    12241 gacgtactgc gactggcacg atgccttcgt gatattgccc atgtaggtct tggcccccgc
    12301 gagcaggccg gcgattgccg actggcctgc gacggccttt atctcaccag tggacgcgtt
    12361 gttggtctcc ggattgagcg cggtggcgaa cgcctgcgcg ccattgctcg tcgtaggatc
    12421 cgtgcccatc gcgacggtcg ctgtgcccgt cgtcgccgac tgggatccgc cataaccgaa
    12481 gccgcgctgg atgtacatca cctgcggcga gtacggcacg aagccggcgt ttgtcggcgt
    12541 tgtcgtcttg gcgttgccgc tcggcgtgac gtacggatag ctttcgctgt agctgccggg
    12601 gttgttgtta taggtcgata gcgagtagtg gctatacgga ttggcaggac tcacttggcc
    12661 gtagtccaag aatacgggcg ccaagccact ggcgtagagg acgtcgttga cctgcgggtc
    12721 gtcgctcgag gtcgagatcg tcatgtacgg gttggtgttc agcgcgttgg cgcccagttg
    12781 gccgctgatc gagttgcagt ccgagtagac agcgttgctg gacggcaggc tctggtatcc
    12841 gtagcagggg ttcgcgacgg tgttcgctga cgcagtgttc gtcaggccga acgagcccga
    12901 cgtcggactc atctggtaga cccacgtcgt gtatagcgat gtcccggagg tcgaatacgt
    12961 gtagatgcca aagttgattg tcgcgccgta ggaattgagc accgacttga tcgccgcctt
    13021 cgtcatgttg aggcggctgg gaccgttgtc gcacttgtaa gagctgttgg tgaggcaggc
    13081 ggcggtgtag tttgccatcc ctcccgacgc ggcctgtacc ggcggcgtga agcctgcggg
    13141 caccgagtat tgcatcggcg agctcgagtt ctgcaacgcg gacagcgacg agcccagcga
    13201 tccactgccc accataatgg cgcctgcggt ggtaccgtcc atcgattcgg agttcgtgat
    13261 ggccagcagc acctgcggcg cggctgctgg cgtcgcggcg ttcagcggcg cgaccggcag
    13321 gccaagcagg gcgatcgtcg ccggaagggc ggctacccgt gtagcacggg cgaacaatgg
    13381 gcgtgtcagc gggtgcatat cttgtagacc gtttcggtga tggaggtggt ggcgctcgac
    13441 gcttcgtaga tgcgcaggta gatgtcgtag tagttcgctg tcaggaaggg gccgatgttg
    13501 cagttggacg tgtcggttcg gcctgtcggc tggaccaccg cttgcacggt gtagcccggg
    13561 ccaatgctgg agccattgtt ggtcaccgtc agggcggcgc agcgcagcgt cgcgtcggta
    13621 ttgccgacgc aactacccca gtaggtactc gcgtcaggtg gtgcagtgcc gggtgagaca
    13681 tcgcgccacc aagccggctg cgctgcccca gtgccaacgc tgagttcgag cggctgaccc
    13741 gtcgtctgcg aattggcgac catcaaggcc tcgacctggg cgagtgcaat gtcgctgatc
    13801 tgcgcatcct tttggcgttg caggttgttg ccgacaagtt gggtcgtcac gatgctgccg
    13861 cgcatcgaca ccaccagtcc cagcatgaat acaagcagga agacgaggac gatgagcagc
    13921 acgtcgccac gcgccggacg cgcgcaagac ggatggccgg gatgcatgga tgcggtcatt
    13981 tcttgcagat gagcccgttg cagttccaga gcgcgttgcg gttggcgatc tccagggtgc
    14041 tcacgacgta gcggtggttc tgttgactgc cgctgctggg aatcgtgacg gcggcgaagc
    14101 cgaagccact ttcgagacaa ggcttcaccg ggctgttcgg cgtcgtcgtg cagaccggaa
    14161 tcgaagagat ggctgcattg ccactcccgc cctgcatgtc gctctcggtg gtgaggctgc
    14221 gtgagacgat cgcgaccttc gtggagcgca ccgtgtccca ttcgttgttc gtcgtcacgg
    14281 tggccgcatc gtggtatgcg gtaatggcgc cggtcttgcc cgggtccacg ccgaagcgca
    14341 cttgcagcga cacgacgccg gcggcgacga tctgcggcgc cgtctgcacc gagtcgtcct
    14401 tgaaggagta ggtggccatc ttgagtaccg ggaacgaata gttcgcgtcc cggtcaacat
    14461 agaagaccac ggtgacggcc gaggtcgagc gaggcgtggt agctactggc atccccggcg
    14521 gcacgtacgt gatgtccacg aactggccct ggaataccgc ggcgtcggtc atggtgccgt
    14581 agcccgcggt ggtgagcgag ctggcgtagc cgttgaggaa cgtgctcggc atcagggcgt
    14641 tgaccgcgct gtcgtccacg tcgggagaac cggaggcggt gctcaatgac gcgatcggca
    14701 gccggaagca cgcgatgcgg ttggtcaatg cgacctggag gatcgccgtg tctccggccg
    14761 tcaggccggt cgtggtctgc atcgggaact tgcccgtcga catcggcgtg taggcggaat
    14821 tcggcgagac cgcgacgata ggtgcattgt tggcatcgaa gccggtcgcg tcgctggacg
    14881 tggttacggc gagaacgtcc gaaggcgtcg gcgtaccgga ggttcctgga taagtcaact
    14941 gaaggccgct agcaaagggc aagtacaggc cgctagtcgc tgccacggca tcgaccgggt
    15001 ggtgcacgaa gtacttgctc gtcgactgcg cacccgagtt gtaggtgaac atctcgttgc
    15061 aagcttgcgg cgagccgccc aggaggaacc ctgcgccggt gacagtccgg ttgatgagat
    15121 cgagtgcggt acgcgcctcg ttgtcccggg tctcggagtc ggcgttgtgt accgtctggg
    15181 tcgcgaggct ctgctgcgct ttcagcatga cacccaccag gatcatcgac agcgccagcg
    15241 cgacgagcag ttcgacgagc gtgaagccgg cggcagcgcc gccgcggcgc gatccggcga
    15301 gtgagggcac gagggtcacg attgactgta ccggtagagg aacatctggc tgcgcgtcgg
    15361 gctcgacgag cttctgacgg agccgacctg cgcccagctg atcgtcagcc gcaccgtgca
    15421 gccggtcgac acggcgcaag cgaggccgga cgacgagtcc gggagcgtct cgatctgcgc
    15481 ggtgccgctg ggcagcaagg tgacgacttg cttcaagaat ggctgcacgg cggccggagc
    15541 tgccgtatag gtgctcgcgt tgtaagttgt tcccccggtc gtggcccacc cggtcgtcgt
    15601 caagacggcc gaattggcct ggatcgcgct ccagaacgta ttgctcagcg gtgcgagcgt
    15661 cgtcacgttc tcgttctgcg tcgccgcggc ggtaacgctg ccgagactgt gggcgacgcc
    15721 aagcatgccc aggccgaaaa taagcgccgc gacaagtacg ttcagcagtg tgaacccaga
    15781 ctggccgcgg gcggcccggc gatgcgaagt ggcgatgcca gctttcacga cagtccctgg
    15841 ttgatgagta ccgtgcccga gagcatgaag atcagcggcc agtccgtgcc gcgactggaa
    15901 tggaaggtct gcaagccgct cttgtcgacg gtgccctggg acgaaaagct gaaggtcacc
    15961 ggctggctca acgcgctgct tcccgtcccc gccgggcagg agagccccgg ggtggacgac
    16021 gacaactgtg cggtggtcat tgaatgcgcc ggatcggtgt ggccgttgac cgacatcgtc
    16081 cagtcgcagc tgcccgcggt ggagaacatc gtcactcgga tgactggcag cccgctggcc
    16141 ccgctgacgt tgagcgagtt tgcatcggaa accccagccg ccgtgcgcgc ccaggcgaag
    16201 tcctgctgca gctttgcaat cacgccgcgc tccttcgcgc ccgtggtcgc gttattgaaa
    16261 tacggaacgc cgagcgccag cgcgatcgcg atgatggcga tcgtcaccat caactcgatc
    16321 atcgtgaagc cggcttggag ggcgagacgt gtcgacatgc tcgtggctcg tgcgcagaag
    16381 ctcagaagca agtgccggac ttgctggcga tccagcaact cgaacttgtc ggatagcctg
    16441 cactcgtggc caccgtcact ttggtgcccg acgcgtccag cgtgtaggtg aacccggtcg
    16501 tcgagttcgt gctacccgtc gccgtgacga gatagccgga ggaggtcagg tgatcgcagg
    16561 tgtacgtgaa attggtggtc gaggtggggc aggtcgcccc tacataagtg cgattgtcct
    16621 ggtagtactg tcccagcgcg agcgcgtccg cagtcatctg gttgaacgcc tccgtcaact
    16681 tgccccgccg aatgtagtcg gtatacgcgg gcagcgcgat gcgcgccaag atggcgatga
    16741 cggctaccgt aatcatcagc tcgatgagcg tgaagccgcg ggcggcggtc gatcggatgc
    16801 gggaggggcg tgcgagggcc atcggcagcg gttcctggct cggttggacg cccagcatgg
    16861 gcgtaattga ttgtcggcaa gcggcgaagc tatcgcggcg cgcggcggat gaacggttga
    16921 aaagaacgac cgaacggatc aacagagacg acgcgatcgc gcgtccgagg ccaagtcagc
    16981 cctcgccgac atcccggccg aggtgctcgc aggccgggtc gtcgcaaagc tcgcagcgcc
    17041 aggccgggcg ctgaggctgg tcgctggcct ccatcaggcg gcgaaacagg ccggagccgg
    17101 cccccgcatg gcgcgcgcgt cgccgcagca acgggcgcag ggcatcgagc gccgcgtcgg
    17161 tacgcgcgcc acccacgccc gagaccagag accaccggag cccccagtcc aagagtcgtg
    17221 cacgcaccgc ggcatcgacg ggcgcgcgaa cgtgcgggcc gtcgcgctgc aggccgtcgg
    17281 cgacccaagg caggtcgagg gcggtgagca gcgtcaaatc gacgccccat tgcgcctcgg
    17341 ccgcgaaggc gtagagcgag caatcgtcga acagcaattc gctgtatacg gccgtcatca
    17401 gcggtgtcgt gtcgcagagg acgacctcga cacccggcgt cgccgcggcg gcgtcgatgc
    17461 gtcgctgctg ttcgcgcgcg aggggggcct gctcgtccgg gcggggtgtg cggtcgttga
    17521 cgtcgcacca ttcccgcagg tactcgccca cccgcacgca gtgcaggccg aagtcgcgcg
    17581 cgagcgcccg ctccagcgcc accgcaagcg tggttttgcc ggtactctcc gcgccgacga
    17641 tggccacgcg cagcgcagta ccggggggct caggccgcgg catgcgaacg gccagaggaa
    17701 gtggcacagg agcgaccgtt caggcggcgc cagacgcgcc agccggcgac cgacagcgca
    17761 gcgaacagcg cgtacagaag cgccgtcagc cacaggcctt tataggcgaa cagcccgacg
    17821 ctgaagacgt tgacggccag ccaggccggc cagttctcga tcagcttgcg tcccagcagg
    17881 aaggtggcgg cgatgctcac caccgtgggc agcgcgtcgc agaagggcac cggcgaatcg
    17941 gtcgtgcggg cgagcagcca accgagcgcc ggccagctcg cgagcgtcgc catgcccacc
    18001 gcgacggccg ggccgcgcgc cagccggtgc accatcagcg cgccgccgtc gcgtccgtgc
    18061 ccgcgtcgcc actgccacca gccccaggcc gacatggcga cgaagaacag ctgcagcgag
    18121 gcttcgccgt agagcttggc gtcggcgaac agcgcggcgt acatcaacga cgtcacgatc
    18181 gcgagcggcc aggcgagcga gcgcaccttc atctcgcaga ccaccatcca caacgacagc
    18241 gcgaaagcga cgacctcgat ccaggtgacg tcggtgcccc aggcggtgaa cgcggtggcg
    18301 aaggcgggcc gcaggaaggc gagcagggcc tggagcatgc gcgctcagcg gccgggcgtg
    18361 agcacgacca ggagctcgtc cggcttcgtc atgcccagct cgtagcgcgc cttctcctcg
    18421 accatctcca ggccttcctt gaggtcgctg acctcggcct ggagctggtc gttgtgctgc
    18481 ttggccacgt cgttggcggc ctgcagcttc tcgagctccc cgcgcagctg catcacgtgc
    18541 gccatgcccg acttgccgac ccagagctcc gcctgcaccc atccgaccag gacgagcagc
    18601 gcgatcgtga cgagtcgcat ggcgggcgct tcgtgggccg gcggcggggc gtcagcgcag
    18661 gttgtagaac gccgcgcggc cggggtagct ggcgatgtcg ccgaggtctt cctcgatgcg
    18721 cagcagctgg ttgtacttcg cgatgcggtc cgagcggctc atcgaaccgg tcttgatctg
    18781 gccggcgttg gtgccgacgg cgatgtccgc gatcgtggag tcctcggtct cgcccgagcg
    18841 gtggctgatg acggcggtgt agcccgcgcg cttggccatc tcgatggcgg cgaaggtctc
    18901 ggtcagcgtg ccgatctggt tgatcttgat caggatcgag ttggcgatgc ccttgtcgat
    18961 gccttccttc aggatcttcg tgttggtgac gaacaggtcg tcgccgacca gctgcacctt
    19021 cttgcccagc gcctcggtca ggtgcgccca gccttcccag tcgccctcgt gcatcgcgtc
    19081 ctcgatcgag atgatcgggt acttgtcgac ccaggtggcc agcatgttgg tccactcggc
    19141 ggccgtcagc tgcaggccct cgccctcgag gtggtacttg ccgtccttgt agaactcgct
    19201 ggcggcgcag tccaggccga tggcgatctg ctcgccggcc ttgtagcccg ccttctcgat
    19261 ggcttccagc agcagctgga tcgcggcctc gtggctcgcg acgctcggcg cgaagccgcc
    19321 ctcgtcgccg acggccgtgc tgatgccgcg gtcggccagg atcttcttca gggcgtggaa
    19381 ggtctcggcg ccgtagcgca ccgcttcgcg gaagctcggg gcgccgatcg gcaggagcat
    19441 gaactcctgg atgtcgaggt tgttgttggc gtgcgcgcca ccgttgatga cgttgatcat
    19501 cggcaccggc atctgcatgc ggcccatgcc gccgaagtag cggtacagcg gcagcccgct
    19561 ttcttccgcg gcggcgcggg cgacggccat cgacacggcc agcgtcgcgt tggcgcccag
    19621 gcggcccttg ttgtcggtgc cgtcgaggtc gatcagcgtc tggtcgagga aggcctgctc
    19681 ggaggcgtcc aggcccagca ccgcttccga gatctcggtg ttgatgtgct ccacggcctt
    19741 gagcacgccc ttgccgaggt agcggccctt gtcgccgtcg cgcagctcga tcgcctcgcg
    19801 cgagccggtc gacgcgccgg acggcaccgc cgcgcggccc atcacgccgc tttccagcag
    19861 gacgtcgcat tcgacggtcg ggttgccgcg gctgtcgagg atctcgcggc cgacgatgtc
    19921 aacgattgca ctcatgtcag tttgctccag gcttttggga ttcttgcgcg cgcccggacg
    19981 ggcggacggc gtcggattct tccagggcgg cgttaccgcg tcacatcaag ctgcgtacct
    20041 catgcgagac gccggcggtt gccgcctccc gcacaccggg accgcgcacg ggcccgcgca
    20101 cgcgcggccg ctccggaggg agcgggcctg agccccttgg gggcggcgaa cggagcgagc
    20161 ccgggccgtc attcacgccg cagccttgtg ggtcgcgtcg gccgccgcgt ggtgggcgag
    20221 cgcggccttg atgaagctcg tgaacagcgg atggccgtcc cagggcgtcg acttgaactc
    20281 ggggtggaac tgcacgccga cgaaccaggg atggacctcc tgcggcagct cgacgatctc
    20341 ggtgagcttc tcgcgctgcg tgagcgcgga gatcaccagg cccgcttcct tcaggcggtc
    20401 gaggtagtgc tcgttggcct cgtaacggtg gcggtggcgc tcggtcacga ccttgccgta
    20461 gatctggctc gcgagcgtgc cgggctcgac gtccgagctc tgcgcgccca gccgcatcgt
    20521 gccgccgagg tcggagttcg cgtcgcgctt ctggatcgag ccgtcgcggt cctcccactc
    20581 gtcgatcagc gcgatcaccg ggtgcggcgt gttcgggtcg aactcggtgg agttggcgtt
    20641 ggcgaggccg gccttgtggc gcgcgtattc gatggtcgcg acctgcatgc ccaggcagat
    20701 gcccaggtag ggcaccgcgt gctcgcgcgc gtactgcgcc gcgaggatct tgccctcgac
    20761 gccgcgcttg ccgaagccgc ccggcaccag caccgcgtcg aacttgccca gctgcgagat
    20821 gttctccggc gtgagcgcct cggagtccac gtactcgatg ttgacgcgcg cgtggttctg
    20881 gatgcccgcg tggcgcagcg cctcgttgag cgacttgtac gagtccgaca ggtccgtgta
    20941 cttgccggcc atcgcgatcg tgacctcgcg ctgcggatgg ttgacctcgt gcacgagcag
    21001 gtcccagcgc cgcaggtcgg ccgggcgcgt cagcagctgc agcttcatgc agatctgctc
    21061 gtcgagcccc tgctcgtgca gcatgcgcgg cacgctgtag atggtctgcg cgtcccagac
    21121 cgagatcacg ccgtgcatcg gcacgttcgt gaacagcgag atcttctcgc gctcgtcgac
    21181 ggggatctcg cggtcggcgc ggcacagcag cacgtcgggc tggatgccga tctcgcgcat
    21241 cttctgcacc gtgtgctgcg tcggcttggt cttcagctcg ccggcggccg cgatgaaggg
    21301 cacgtaggtg aggtgcacga acgccgagtt cgtgggcccg agcttgagcg ccatctggcg
    21361 caccgcctcg aggaagggca gggactcgat gtcgcccacc gtgccgccga cctcgcagat
    21421 cgcgacgtcg acgccggcct cgctcgcgcc gcggcgcacg aactcctgga tctcgttggt
    21481 gacgtgcggg atgacctgca ccgtcttgcc gaggtagtcg ccgcggcgtt ccttctcgag
    21541 caccgacttg tagatctggc cggccgtgaa gttgttgctg cgcttcatcc gcgtggtgat
    21601 gaagcgctcg tagtggccga ggtcgaggtc ggtctccgcg ccgtcgtcgg tcacgaagac
    21661 ctcgccgtgc tggaaggggg acatcgtgcc cggatcgacg ttgaggtacg gatccagctt
    21721 gatgagggtg accttgaggc cgcgcgattc gaggatcgcg gccagggaag ccgaagcgat
    21781 gcccttgcca agcgaggaca ccacgccgcc ggtgacgaag acgtacttgg tcatgtcagg
    21841 ggacacccgt gccggaccgc ggggcacgca cgaatgccgt ggtggtaaag ctggattata
    21901 ggcccccgcg caggtgcgtc cgaagacggg gccgcgtgcc gggccgcccc gcgctcagcg
    21961 gcgcgccgac ggcggccgca tcgacgcgag cagctccagc acgagcagcg ccgtgtcctc
    22021 gggccgctcc atcgggtaca ggtgcgtgcc gtcgatccag cgccagcgct cccccgcaag
    22081 cgcgcgcgag gcggcggcgc cggcctggcg catctccgtc gagcgggtgc cggcgatgaa
    22141 gccgacggga cagcgcggcg gatgcttgcg cagcagcgcg ggcacgtggt gcggcagcgt
    22201 gttgtagatg cgcgtctcga cctcgcgcgg gatgccgagg acgatcctgc gcggcgtgtc
    22261 ggcggcgccg ccatcgtccc cggggtcgtg cgcgggctcg gccgcagcct cgtcgaagcc
    22321 ggcatgcacg taggcgtcga gcatgcgcgg atcccagcgc gcgaacgcgg ccttcgccgc
    22381 gaagtgcgcc cgcacggcct cgcgcgacgg ccactcgtgg cgccgtcggc gcgagatggc
    22441 gcccggcccg acgcgctcga tcagccgcat gcgcttggcc atgtgcaggc tgtgcgcgcg
    22501 ccagccggcg accaccggcg agtcgagcat cacgaggccg ccgacgacgt cgggtcgccg
    22561 gcaggcggcg agcagcccga cgaggccgcc gagcgaatgc ccgaccacca tcgcgccggc
    22621 gagctcgcgg gcgactatga agtccaccag ctggtcgcgc agccgcggcc agttgctcgt
    22681 gacggggtag cggctgtcgt ggctgaagcg ctccggcgcc tcgacgcgcc agccgcgcgc
    22741 acgccagagc gcgaagatcg gctcgtaggt gcccgccggg aagccgttgg catgagcgaa
    22801 gacgatggtg tccgtcgcgg tgccgatacg cggcgccttc atcgccggct acaccacctt
    22861 ctcgtcggga ttgcggatct tcttgagctc gtcgtagcgc ggctcgccga cgatcagcgc
    22921 gttgtgcgag ccggcggcgt tgggatccca ctgcggatcc tccatgctct cgaagacgtc
    22981 gcgcaggcgc tggccccacg agctgcggat catgcggaag tacgggttct gctcgtcgat
    23041 gcacacgatc tggtcggact gcagcctgcc ctgctcgtag acgacgaggt ccagcggcag
    23101 cccgaccgac aggttggact tcagcgtcga gtccatcgac accagcgcgc acttggcggc
    23161 ctcgtcgagc ggcgtgcccg gctccagcac gcggtcgagg atgggcttgc cgtacttcga
    23221 ctcgccgatc tggaagaagc aggtctcgcg cgtcgcctcg atgaagttgc cggccgagta
    23281 gacgaggaac agccgcatcg cctcgttgcc gatctggccg ccgaagatga agctggcgac
    23341 gaagtccatg ccgtggttct tgaggctggg cgcgtcgttc tcgtagacgc ggcgcaccgc
    23401 cgagccgagc acgcgcgcgg cgtcgaacat gctggtcgcg ttccagatcg tcagcggctc
    23461 gcggtcgccg tcgggcagct gctcgacctg caggatctcg cgcaccgact gcgagatcga
    23521 gaggttgccc gccgagagca gcaccatgaa gcggtcgccg ggcttctcgt agacgatcat
    23581 cttgcggaag gtgctgatct ggtcgaggcc cgcgttggtg cgcgagtccg acaggaacac
    23641 gaggccggcg ttgagccgga tgccgacgca ataggtcatg ccgttatctt ggtcgtcgaa
    23701 cgatggagtt ggcaagtatc gcgcgcgcgg tcagtccttg ggcagcaggg ccttcaggcg
    23761 gtagagcgct tcaagcgcct cgcgcggggc gagccggtcg ggatcgatgg ccgccagcgc
    23821 ggcctcgagc cgcgtcgggc cggcggccgc cgcggcg
//
LOCUS       DASVLN010000726         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_199052, whole genome shotgun sequence.
ACCESSION   DASVLN010000726 DASVLN010000000
VERSION     DASVLN010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199052"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VF453_17210"
     CDS             complement(<1..212)
                     /locus_tag="VF453_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689453.1"
                     /translation="MRPDLTLVLADSEVRAVHADATGMQVHFAAAKSLRDGVAGWQAG
                     VTLALPGARWAGEPSGAFGRLAEGRLR"
     gene            366..1274
                     /locus_tag="VF453_17215"
     CDS             366..1274
                     /locus_tag="VF453_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7689454.1"
                     /translation="MRPDFDRRDFLCLAGVGAGVVFASGLAGCASRAFAADASATQRD
                     FHFVQLSDSHWGFNGPAVNPESRTTLPRAVAAVNALKDPPDFIVFTGDLTHTTDDPQE
                     RRKRLREFRAIVADLEVKDVRFLPGEHDASLDRGAAFQEFFGPTHYAFEHDGVRFIAL
                     DNVSDPLARLGDAQLAWFASELAKVDKDAPLVVLAHRPLFDLAPQWDWATRDGDQAVA
                     QLMPFTNVTVFYGHIHQEHHHMTGHIPHHAATSLIFPLPAPGSQPDRTPVKWDATQPF
                     RGLGWREVERHGDRPFVLDQHAVQGA"
     gene            1276..1647
                     /locus_tag="VF453_17220"
     CDS             1276..1647
                     /locus_tag="VF453_17220"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX7689455.1"
                     /translation="MRRRQLLNAGAALGASTLVALAARAAAQPPAPRAIKVVARKFVF
                     IPNEIVVKKGESVLLEFTAPEVAMGFFAPALGLRTLIVPGETARVAWRADAAAGKYDF
                     LCDVFCGDGHEGMNGHLVVQA"
     gene            1664..>2184
                     /locus_tag="VF453_17225"
     CDS             1664..>2184
                     /locus_tag="VF453_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7689456.1"
                     /translation="MKDDRHDRFAAQVLPHLGAAYNLARWLVRDGHDAQDVVQDAMLR
                     ALRHFDGLRGDARPWLLAIVRNAAFAWLGARRPEEVALADEALDAAIAIHAAQAPAAD
                     PEALAIERSDKRAIDAAIAALPLVFREAIVLRELEELPYRDIARITDVPLGTVMSRLA
                     RGRGLLAAALRPAG"
BASE COUNT          274 a          838 c          774 g          298 t
ORIGIN      
        1 cgcaggcggc cttcggcgag ccggccgaag gcgccggacg gctcgcccgc ccagcgcgcg
       61 cccggcaggg ccagcgtgac gccggcctgc cagcccgcca cgccgtcgcg caggcttttc
      121 gcggcggcga agtgcacctg catgccggtc gcgtccgcgt ggacggcgcg cacttcggag
      181 tcggcgagga cgagcgtcag gtcggggcgc atgcgcgggc gtcgggggat ggacgatggg
      241 cgaggccgcg attctggcat cgcgcctgac gttttcgccg ccgcgggaat aaagcggcgc
      301 cgcggccgat cctgcactcc gaccgctcgc gccgaccgcg gcggcatcca accgaggagc
      361 cctccatgcg acctgacttc gatcgccgcg acttcctgtg cctggccggc gtcggcgcgg
      421 gcgtcgtgtt cgcctccggc ctggccggct gcgcgagccg cgctttcgcg gccgacgcct
      481 ccgccaccca gcgcgacttc cacttcgtgc agctgtccga cagccactgg ggcttcaacg
      541 gcccggccgt gaatcccgag tcgcgcacca cgctgccgcg cgcggtcgcc gcggtcaacg
      601 cgctgaagga tccgcccgac ttcatcgtct tcaccggcga cctgacgcac accaccgacg
      661 atccgcagga gcggcgcaag cgcctgcgcg agttccgggc catcgtcgcc gacctggagg
      721 tcaaggacgt gcgtttcctg cccggcgagc acgacgcctc cctcgaccgc ggcgccgcgt
      781 tccaggagtt cttcggcccg acgcactacg cgttcgagca cgacggcgtg cgcttcatcg
      841 cgctggacaa cgtctccgac ccgctcgccc ggctgggcga cgcgcagctc gcgtggttcg
      901 cgtcggagct cgcgaaggtg gacaaggatg cgccactggt cgtcctcgcg caccggccgc
      961 tgttcgacct cgccccgcag tgggactggg cgacgcgcga cggcgaccag gccgtcgcgc
     1021 agctgatgcc gttcacgaac gtcaccgtgt tctacggcca catccaccag gagcatcacc
     1081 acatgaccgg ccacatcccg caccacgcgg cgacctcgct gatcttcccg ctgcccgcgc
     1141 ccggctcgca gcccgaccgc acgccggtga agtgggacgc cacgcagccg ttccgcggcc
     1201 tgggctggcg cgaggtcgag cgccacggcg accggccctt cgtgctcgac cagcacgccg
     1261 tgcagggggc ctgagatgcg ccgccgccaa ctgctcaacg ccggcgccgc gctcggtgcg
     1321 tcgaccctcg tcgcgctcgc cgcccgcgcc gcggcgcagc ctcccgcgcc gcgcgcgatc
     1381 aaggtcgtcg cgcgcaagtt cgtgttcatc ccgaacgaga tcgtcgtgaa gaagggcgag
     1441 tcggtcctcc tcgagttcac cgcgcccgag gtcgcgatgg gcttcttcgc gcccgcgctc
     1501 gggctgcgca cgctgatcgt gccgggcgag acggcgcgcg tggcgtggcg cgcggatgcg
     1561 gcggccggga agtacgactt cctgtgcgat gtattctgcg gcgacggcca cgaggggatg
     1621 aacgggcatc tcgtcgtgca ggcctgaacc ttctcgacgc cggatgaagg acgaccgcca
     1681 cgaccgcttc gccgcccagg tgctgcctca cctgggcgcg gcgtacaacc tcgcgcgctg
     1741 gctggtgcgc gacggccacg acgcgcagga cgtcgtgcag gacgcgatgc tgcgcgcgct
     1801 gcgtcacttc gacggcctgc gcggcgacgc gcggccgtgg ctgctggcca tcgtgcgcaa
     1861 cgccgccttc gcgtggctgg gcgcgcggcg gcccgaggag gtcgcgctcg cggacgaggc
     1921 gctggatgcg gccatcgcga tccacgccgc gcaggcgccg gcggccgatc cggaggcgct
     1981 ggccatcgag cggtccgaca agcgcgcgat cgacgccgcg atcgcggccc tgccgctcgt
     2041 cttccgcgag gccatcgtgc tgcgcgagct ggaggagctg ccctaccgcg acatcgcccg
     2101 catcaccgac gtgccgctgg gcaccgtgat gtcgcgcctc gcgcgcggcc gcggcctgct
     2161 cgccgccgcg ctgcggcccg ccgg
//
LOCUS       DASVLN010000727         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_148208, whole genome shotgun sequence.
ACCESSION   DASVLN010000727 DASVLN010000000
VERSION     DASVLN010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148208"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /gene="groL"
                     /locus_tag="VF453_17230"
     CDS             complement(<1..1508)
                     /gene="groL"
                     /locus_tag="VF453_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7689457.1"
                     /translation="MAAKDVIFGADARARMVEGVNTLANAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKLQNMGAQMVKEVASKTSDNAGDGTTTATVLAQAVVRE
                     GMKYVAAGMNPMDLKRGIDKAVTALVEQLKKASKPTTTSKEIAQVGTISANSDESIGK
                     IIADAMDKVGKEGVITVEDGKSLANELDVVEGMQFDRGYLSPYFINNPEKQSAILDNP
                     FVLLFDKKISNIRDLLPTLEAVAKAGRPLLIIAEEVDGEALATLVVNTIRGILKVVAV
                     KAPGFGDRRKAMLEDIAILTGGKVIAEEVGLSLEKVTLADLGQAKRIEVGKENTTIID
                     GNGAAADIEARVKQIRIQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALLRARQLIGELKGDNADQDAGIKLVLKAIEAP
                     LREIVYNAGGEASVVINKVLEGKGNFGFNAANDTYGDMIEMGILDPTKVTRTA"
     gene            complement(1640..1930)
                     /gene="groES"
                     /locus_tag="VF453_17235"
     CDS             complement(1640..1930)
                     /gene="groES"
                     /locus_tag="VF453_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7689458.1"
                     /translation="MKLRPLHDRVVVKRLEQETKTASGIVIPDAAAEKPDQGEVLAVG
                     PGKRNDKGDFVALNVKVGDRVLFGKYSGQTVKVDGDELLVMREEDLFAVVEK"
     gene            2258..2431
                     /locus_tag="VF453_17240"
                     /pseudo
     CDS             2258..2431
                     /locus_tag="VF453_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          353 a          772 c          819 g          487 t
ORIGIN      
        1 gcggtgcggg tcaccttggt cggatccagg atgcccatct cgatcatgtc gccgtaggtg
       61 tcgttggcgg cgttgaagcc gaagttgccc ttgccttcca gcaccttgtt gatcacgacc
      121 gaggcttcgc cgccggcgtt gtagacgatc tcgcgcagcg gggcttcgat ggccttcagc
      181 accagcttga tgccggcgtc ctggtcggcg ttgtcgccct tgagctcgcc gatcagctgg
      241 cgcgcgcgca gcagcgccac gccgccgccg gcgacgatgc cttcctcgac ggccgcgcgc
      301 gtggcgtgca gcgcgtcctc gacgcgggcc ttcttctcct tcatctcgac ttcggtggcg
      361 gcaccgacct tgatgacggc cacgccgccg gccagcttgg ccacgcgctc ctggagcttc
      421 tcgcggtcgt agtcgctggt ggcttcctcg atctggatgc ggatctgctt gacgcgggct
      481 tcgatgtcag cggcggcgcc gttgccgtcg atgatcgtcg tgttctcctt gcccacttcg
      541 atgcgcttgg cctggccgag gtcggccagc gtgaccttct cgagcgagag gccgacttct
      601 tccgcgatga ccttgccgcc cgtcaggatg gcgatgtctt ccagcatggc cttgcggcgg
      661 tcgccgaagc ccggggcctt gacggcgacg accttcagga tgccgcggat cgtgttgacg
      721 accagggtcg ccagcgcttc gccgtcgact tcttcagcga tgatcagcag cggacggccg
      781 gccttggcga cggcttccag cgtcggcagc aggtcgcgga tgttcgagat cttcttgtcg
      841 aagagcagga cgaacgggtt gtccaggatg gccgactgct tctcgggatt gttgatgaag
      901 tacggcgaca ggtagccgcg gtcgaactgc atgccttcga cgacgtcgag ctcgttggcc
      961 agcgacttgc cgtcctcgac cgtgatgacg ccttccttgc cgaccttgtc catcgcgtcg
     1021 gcgatgatct tgccgatcga ctcgtcggag ttggccgaga tcgtgccgac ctgggcgatt
     1081 tccttggacg tggtggtcgg cttcgaggcc ttcttcagct gctcgaccag ggcggtgacc
     1141 gccttgtcga tgccgcgctt caggtccatc gggttcatgc cggcggccac gtacttcatg
     1201 ccttcgcgca cgacggcctg ggccagcacg gtggcggtgg tggtgccgtc accggcgttg
     1261 tccgaggtct tggaagcgac ttccttgacc atctgggcgc ccatgttctg gagcttgtcc
     1321 ttcagctcga tctccttggc gaccgagaca ccgtccttgg tgacggtggg cgcgccgaac
     1381 gagcgctcga gcacgacgtt gcgacccttc gggcccaggg tgaccttgac cgcgttggcc
     1441 agggtgttca cgccttcgac catgcgggcg cgggcgtccg cgccgaagat gacgtcttta
     1501 gcagccatga gaaattctcc gggaaattcg ttgattcagt gacgggggcg gcggcgcgag
     1561 ggcacgccgg ggcgatggcc gcccgaccgg ttgccggtcg gcccggcgag gcgcgcgcgg
     1621 cgccccgcag ccttggaaat tacttctcga cgacggcgaa gaggtcttcc tcgcgcatca
     1681 ccagcagctc gtcgccgtcg accttgacgg tctggccgct gtacttgccg aacagcacgc
     1741 gatcgccgac cttgacgttc agggccacga agtcgccctt gtcgttgcgc ttgcccgggc
     1801 cgacggccag gacttcgccc tggtccggct tctcggcggc ggcgtcgggg atgacgatgc
     1861 ccgaggcggt cttggtctct tgttccaggc gcttgacgac cacgcggtcg tgcaacggac
     1921 gaagcttcat ttcatctcct tgatgcgttc gcagtaagac ttcgcagcgg atcgcccgga
     1981 agtgagcgtc cgccgacaaa gaagaggtgg ccagtgccgg cgaccgtctc atcagtcgct
     2041 agcactcgca gcggttgagt gctagcagcg attatagggc ggggagatga cgtttcaaga
     2101 ggggaggcgg cgggagggct tccgcgccgc tcagtgttgg tcaccgttca agtcagaact
     2161 tcttccgttg aagtctgaag ttgatctcag caccggtgat gtcccggaag cggtgtaagt
     2221 ctccaagccg aggcgccctg aaacaggggc ggccaaccgc cgcggactac gcggcgtcaa
     2281 actggtgatt agcgacagcc acgagggcct caaggccgcg gcctcgaagg tactcaaagc
     2341 gacgtggcag cgctgccgcg tgcacttcat gcgcaacgcg ctggccttcg ccaacaagac
     2401 gcagcggcgg atggtgtcgg cggcgatcgg c
//
LOCUS       DASVLN010000728         9237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_469563, whole genome shotgun sequence.
ACCESSION   DASVLN010000728 DASVLN010000000
VERSION     DASVLN010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469563"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VF453_17245"
     CDS             complement(<1..473)
                     /locus_tag="VF453_17245"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7689459.1"
                     /translation="MHDEPPPSPDAAPPDEDSLPLPQAVRALFADLPGIVSDRVHLLA
                     LELQRASMAFGRIVALALLAAILVATAWTALWCAIAGGLIALGIQWPWVALLVLFVNL
                     AAAVWALMQVKALAPLLALPATLRRLTDAGSREKQDERQRESEAAARRAYAAAAA"
     gene            complement(499..879)
                     /locus_tag="VF453_17250"
     CDS             complement(499..879)
                     /locus_tag="VF453_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689460.1"
                     /translation="MSPATPASGGSGSRVGDAAASTAAGAHDAVDAAAAAEKDVMRRV
                     VAGAHEAIDRIADKAIPAVERLRGTYDDATESLKARADQALDMKDEWTESLRATVREH
                     PLASIATALAVGVILARLTSGNDR"
     gene            complement(1318..1914)
                     /locus_tag="VF453_17255"
     CDS             complement(1318..1914)
                     /locus_tag="VF453_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689461.1"
                     /translation="MSPIQKTFTALAAAAALASVGYATAQSTDSASTIDTSGQVQQQT
                     TTGTSDTGASGMNATPSSTTTTDVNGATTTTTTTTDTTTQTLPAPPTGVVELAKPKNT
                     VEPETKQPNGAQGMENSVMTTGMTNGITRPSIGDHATPPIPGQPEVLAVTTERTTQTT
                     TTPAPAPAPEAAPAPAPEPAAAPEPAPEPMPAPKADRN"
     gene            complement(1989..2648)
                     /locus_tag="VF453_17260"
     CDS             complement(1989..2648)
                     /locus_tag="VF453_17260"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7689462.1"
                     /translation="MTDPARPSDLAAGEDLSTGSAGGDQPTGCAGESQAAGCADHWPR
                     DDVLDALDVLAEGMARSRALAEECRRFARHVTPDARMPTVAEALCRAVRRLAVLEEEL
                     FYPAAREALAGSPVVDLAALEHATARQIIARMQETDPRSPCYEALVVALADCVDRHAR
                     HERSELFPRLREAQLDLASLAAAMHARRRELEAMPMSPYAALEGASGASGGGSAGTVV
                     A"
     gene            2930..>9237
                     /locus_tag="VF453_17265"
     CDS             2930..>9237
                     /locus_tag="VF453_17265"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX7689463.1"
                     /translation="MTGHARTLAALLQQARDWFDTHAHGHSAAGGQEGEEPLRAALFS
                     SDQMVAHGRLLASRHRLSQRHRPDPLLARLADNERSLEEVAAALRHAVARDRAITPAA
                     EWLLDNMYLIDEEIRTARRHLPRGYSRELPRLAGKTEESDGTTPRVYDLALEAIAHTD
                     GHLGRGTLSRFLAAYQGVQPLKLGELWAFPIMLRLALIENLRRVAVRVAASLQQRDLA
                     QQWAERMLQAAEDRPSDLILVIADMARSDLTPTSAFVAEFARRLQGQGAALALPLTWM
                     EQRLADVDETIEHLVHVENQTQAASQVSVANSIGSLRLLGATHWPEFVETLSSVEQVL
                     REDPSGVYGRMDFGTRDNYRHAVEAIARISPANEPAVAQAAIELASNASQRLARTPAP
                     DGDEREAHVGYWLVGPGRATLERAVDARRSRFGPAPGRSPLPAYIGSIALFALLMSFG
                     PLRQATDEMDLRLWSPLGIAVGLLLVIATSQLAISLVNWIVTLGVTPHALPRMDYTQG
                     IAPRARTLVVVPTLLGRLDDVDALLDSLEVRYLANRDPHLQFALLTDLRDAPEEHMPG
                     DDELVERAAAGIEALNRRYDRAEDEGQRTRPFLLLHRPRRWNAKEGVWMGHERKRGKL
                     ADLNALLRARPGAAERFERLVGDTLSLQDVRYVITLDTDTQLPRDAAAEMISAMDHPL
                     NRPRWGDGARADVVVDGYGILQPRVGVNLPSVRRSGYARIFGGEPGIDPYTRAVSDVY
                     QDLFGEGSFIGKGIYDVDAFERAAGSMPENRILSHDLIEGCYARSGLLSDVQLIEDVP
                     SRYAVDMARRHRWVRGDWQLIGWLRRRLHLLPGKPRNPLSALSRWKLLDNLRRSLVPT
                     ALVALAVVGWAWLPEPLMWTLRVVVIVGIVPFAAHVMGLLRTPLDWMASSHSPARLQP
                     IGRQLLQMVHSLACLPTEAAATLDAIGRALWRMVRGRRLLEWTASSDIPRGSAPGSLA
                     ALRDSIRALWAGPVAAIAIAALLAGVRPWALPAAAPLLLLWLWSPLIVWWADRPLAEP
                     RSELDAGQRHYLRRVARRTWAFFETFVGADDNHLPPDNVQLHPVARVAHRTSPTNIGF
                     SLLANLTARDLGYATLDQVLTRIAATVTTLERLERHRGHFYNWYDTQSLLPLAPRYIS
                     TVDSGNLVAQLMTLRMALLAMPAEAPFSPNWCHGLADLVGLVRETLPAGASVGLVDKL
                     DRQLAALCVVPPTTAATLKSMLETVDGLARQLVAAVESALSGAAARGEHPPDAAEDHN
                     GEALRWAQLLVAQCEAGLDEFNALGGLAPPLPDDATHAAQEEARKAPIPSLAALAGAG
                     VGTAAVYLDRIAELARRVDAITEMEQQFLYDDRRHLMTIGYNVDERRLDASHYDLLAS
                     EARLGIFAAIARGQLPQESWFALGRLLTSVDGDPVLMSWSGSMFEYLMPPLVMPEFEH
                     TLLAQTSRAAVRRQIEYGRERGVPWGISESGYNATDTALNYQYRAFGVPGLGLKRGLG
                     DELVIAPYATAMATMVEPVAACANLQRLQTLGAAGEYGFHEAVDYTPMRVPRGQTKSV
                     VRSFMAHHQGMSLLAIEQALGTSRMQAHFASDPRVQATLTLLHERVPKDVVPVVPEPT
                     MEPSAPRSVSTATETALRVFTDPQTPTPEVQLLSNGSYHVMVTQSGAGYSRWRDLAVT
                     RWREDATRDDWGSFCYLRDVDTGEFWSTAYQPTVRKPEDYQAIFTEGRAEFRRRDAGL
                     DTHVEIAVSPEDAIELRRVRLKNGTRRRRRIEITSYCEVVLMTPAADVQHPAFGKLFV
                     QTELVDGVPALLANRRPRAEKDPSPWMFHLMAVHTPNGPDTVSPVTHETDRARFIGRG
                     QTLRAPAALLGADALSNTQGSVLDPCAASRCTVVLEPDQTVTVDLVIGVADKREDCLG
                     LIEKYRDRRLAERVFELAWTHSQVLLRQLNASESEAQMFARLAGAVLFSQGALRADPA
                     VIRQNRRGQSGLWGYAISGDLPIVLVQISDAANLELVRQLVVAHAWWRLKGLAVDLVI
                     WNEERDVYRQRLNEQVMGLIAAGVEAHVVDRPGGIFVRQADQIPHEDRVLLLSVARAV
                     LSDRQGTLAEQLARRLRTERRTTPRPLRP"
BASE COUNT         1182 a         3474 c         3336 g         1245 t
ORIGIN      
        1 tgggccgccg cggccgcata ggcgcgccgg gcggccgcct cggactcgcg ctggcgctcg
       61 tcctgtttct cgcgcgagcc ggcgtcggtc aggcgccgca gggtggccgg cagcgccagc
      121 agcggcgcga gcgccttgac ctgcatcagc gcccacacgg ccgcggcgag gttcacgaac
      181 agcaccagca gcgccaccca cggccattgg atcccgagcg cgatcagccc gccggcgatg
      241 gcgcaccaca gcgccgtcca cgccgtcgcc accaggatcg cggcaagcag cgcgagcgcc
      301 acgatgcgcc cgaaggccat cgaggcgcgc tgcagctcca gcgccagcag gtggacgcgg
      361 tccgagacga tgcccggcag gtccgcgaac agcgcgcgca ccgcctgcgg caggggcagc
      421 gagtcctcgt cgggcggggc ggcgtctggc gagggcggcg gctcgtcgtg catgcggcgg
      481 tgtggttggc gtggaggttc agcggtcgtt gcccgaggtc aggcgggcga ggatcacgcc
      541 caccgccagc gcggtcgcga tcgaggccag gggatgctcg cgcacggtgg cgcgcaggct
      601 ctcggtccac tcgtccttca tgtcgagggc ctggtcggcg cgcgccttca ggctctcggt
      661 cgcgtcgtcg taggtgccgc gcaggcgctc gacggccggg atggccttgt cggcgatgcg
      721 gtcgatcgcc tcgtgggcgc cggcgacgac gcgacgcatc acgtccttct cggcggccgc
      781 ggcggcgtcc accgcatcgt gggcgcctgc ggcggtggat gcggcggcgt cgccgacgcg
      841 cgagcccgat ccgcccgatg cgggcgtggc cggcgacatg gaactcggcg tactcgtgcc
      901 gctcttcgga aatgcgtttt cggcggaagg attgctggtg ttggccatgg aggtctccgg
      961 gttggacatc aggcgggcaa ggggcccggt cgccgcgcga actggcaacc ggtgtgccag
     1021 cccgacagcg gccggaccgg catgcgaacg acgcgcgccc cgcgggtgca ggcccggccc
     1081 gggccctcgt ccacataccc tgtcaggtat gcaggatgca ccgccgtccg gacgcgcgag
     1141 ccggtagaac ttgctgcccg ggcggcgctc gcagttgacc acgctgcccc cgcgccgtca
     1201 acgcgcggcg acctggccgc gcctgcctcg caccaaagga aacgggcccg cgaggggccc
     1261 gttcctggcc gctcgcccgc gaggggcgag agcggccgcc acgaaaggag atcgcgatca
     1321 gttgcggtcg gccttgggcg ccggcatcgg ttccggagcc ggttcgggcg cggcggcggg
     1381 ttccggcgca ggagccgggg cggcctcggg cgcgggggcc ggcgccggcg tggtcgtcgt
     1441 ctgcgtcgtg cgttcggtgg tcacggccag gacttccggc tggcccggga tgggcggcgt
     1501 tgcgtggtcg ccgatgctcg ggcgggtgat gccgttggtc atgcccgtcg tcatcacgct
     1561 gttttccatg ccctgcgcgc cattgggctg cttggtttcg ggctccacgg tgttcttcgg
     1621 cttggccagt tccacgacgc cggtgggcgg tgccggcagc gtctgcgtgg tcgtgtcggt
     1681 ggtggtggtc gtcgtggtcg tcgcgccgtt gacgtccgtg gtcgtcgtcg acgacggcgt
     1741 cgcgttcatc ccggacgcgc cagtgtcgct cgtgccggtc gtcgtctgct gctggacctg
     1801 gccgctcgtg tctatcgtcg acgcgctgtc cgtggactgc gcggtggcgt agccgacact
     1861 ggcgagagcg gcggcggcgg cgagtgcggt gaaggtcttc tggatcgggg acatggctgg
     1921 gtactcctgt gtgtagctat gaagcggggc gtcgtcgctg cggaaccggc cccggctggc
     1981 acacggaatc aggcaacgac cgtgcccgcg gaacccccgc ctgaagcgcc gcttgcgcct
     2041 tccagcgcgg cgtacggcga catcggcatg gcctcgagct cgcgccggcg cgcgtgcatc
     2101 gcggcggcga gcgacgcgag atccagctgc gcctcgcgca ggcgcgggaa cagctcgctg
     2161 cgctcatggc gcgcgtggcg gtcgacgcag tcggcgagcg ccacgaccag cgcctcgtag
     2221 cacggcgagc gtgggtcggt ctcctgcatg cgcgcgatga tctggcgcgc ggtcgcgtgc
     2281 tcgagcgccg cgagatcgac caccggcgag cccgcgagcg cctcgcgcgc cgccggatag
     2341 aacagctcct cctccagcac cgccagccgg cgcaccgcgc ggcacagcgc ctcggccacg
     2401 gtgggcatgc gcgcgtcggg cgtgacgtgg cgcgcgaagc ggcggcattc ctcggcgagc
     2461 gcgcggctgc gggccatgcc ctcggcgagg acgtccagcg cgtccaggac gtcgtcgcgg
     2521 ggccaatgat cggcgcagcc cgcggcttgg gattcgccgg cgcagccggt gggctgatcc
     2581 ccgccggcgc tgccggtgga cagatcctcg ccggcggcga ggtcggaggg gcgtgcggga
     2641 tcggtcatgg cggcggcagg cggtggaatg gcgggatccg gcgcggtgcc gggacgtgcg
     2701 gcggcgcgag cgccgcgacg cccgccaccg tccagctgca gggggcaagc gctgtgccgc
     2761 gcacggcctg cgagggtgcc gtcgccgcac ccggcgggtt gccgcaactt ttccccggcc
     2821 gcgcagggcg gccgcatcgg cgcgcgatct ccgcccggga caccctcggg cccgcgaatt
     2881 gcctgcgcgg cggctcacgg ccgctttccg gccccaagga caagccctga tgacgggaca
     2941 cgcacgcacg ctcgcggcct tgcttcaaca agcccgcgac tggttcgaca cgcatgcgca
     3001 cggccactcc gcggccggcg ggcaggaggg agaggagccg ctgcgcgcgg ccctgttcag
     3061 cagcgaccag atggtcgccc atggccggct gctcgcaagc cgccaccgcc tgtcgcagcg
     3121 ccaccggccc gacccgctgc tcgcgcgcct ggccgacaac gagcgcagcc tcgaggaggt
     3181 cgccgccgcg ctgcggcatg cggtggcgcg cgaccgcgcg atcacgccgg cggcggagtg
     3241 gctgctggac aacatgtacc tcatcgacga ggagatccgc accgcccgcc ggcacctgcc
     3301 gcgcggctac agccgggagc tgccccgcct cgccggcaag accgaggaat ccgacgggac
     3361 gacgccgcgc gtctacgacc tcgccctcga ggccatcgcg cacaccgacg gccacctggg
     3421 ccgcggcacg ctgagccgct tcctcgccgc ctaccagggc gtgcagccgc tcaagctggg
     3481 cgagctgtgg gcgttcccga tcatgctgcg gctcgcgctg atcgagaacc ttcgccgggt
     3541 ggccgtcagg gtggcggcct cgctgcagca gcgcgacctc gcgcagcagt gggccgagcg
     3601 catgctgcag gcggccgagg atcgcccgag cgacctcatc ctcgtcatcg cggacatggc
     3661 gcgctccgac ctgacgccca ccagcgcctt cgtcgccgag ttcgcgcgcc gcctccaggg
     3721 ccagggcgcg gcgctcgcgc tgccgctcac ctggatggag cagcgcctgg ccgacgtcga
     3781 cgagacgatc gagcacctcg tgcacgtcga gaaccagacc caggccgcgt cccaggtctc
     3841 ggtggccaac agcatcggca gtctgcgcct gctcggggcg acgcactggc ccgagttcgt
     3901 ggagacgctc agcagcgtcg agcaggtgct gcgcgaggat ccgagcggcg tctacggccg
     3961 gatggacttc ggcacccgcg acaactaccg ccacgccgtg gaagccatcg cgcgcatcag
     4021 cccggccaac gagccggcgg tcgcgcaggc ggccatcgag ctcgcgagca acgcgtcgca
     4081 gcggctcgcg cgcacgcccg cgcccgacgg cgacgagcgc gaggcccacg tcggctactg
     4141 gctcgtcggc cccggccgcg ccacgctgga gcgcgccgtc gacgcgcgcc gcagccgctt
     4201 cgggcccgca ccgggacgct cgcccctgcc cgcctacatc ggctcgatcg ccctgttcgc
     4261 cctgctgatg tccttcgggc cgctgcgcca ggccaccgac gagatggacc tgcggctgtg
     4321 gtcgccgctc ggcatcgcgg tgggcctgct gctggtgatc gcgaccagcc agctcgcgat
     4381 ctcgctggtc aactggatcg tgaccctggg cgtgacgccg cacgcgctgc cgcgcatgga
     4441 ctacacgcag ggcatcgcgc cgcgcgcgcg cacgctggtg gtcgtgccga cgctgctggg
     4501 ccggctcgac gacgtcgacg ccctgctcga ctcgctggag gtgcgctacc tcgccaaccg
     4561 cgacccgcac ctgcagttcg cgctgctgac cgacctgcgc gacgcccccg aggaacacat
     4621 gcccggcgac gacgagctgg tcgagcgcgc cgccgccggc atcgaggccc tgaaccgccg
     4681 ctacgaccgc gccgaggacg agggccagcg cacgcggccc ttcctgctgc tgcatcgccc
     4741 gcggcgctgg aacgccaagg aaggcgtctg gatgggccac gagcgcaagc gcggcaagct
     4801 cgccgacctc aacgccctcc tgcgcgcccg gcccggcgcg gccgagcgct tcgaacgcct
     4861 cgtcggcgac acgctgtcgc tgcaggacgt gcgctacgtg atcacgctgg acaccgacac
     4921 ccagctcccg cgcgacgccg ccgccgagat gatctcggcg atggaccatc cgctcaaccg
     4981 cccgcgctgg ggcgacggcg cgcgcgccga cgtcgtggtc gacggctacg gcatcctgca
     5041 gccgcgcgtc ggcgtcaacc tccccagcgt gcggcgctcc ggctacgccc gcatcttcgg
     5101 cggcgagccg gggatcgatc cgtacacgcg cgccgtctcc gacgtctacc aggacctgtt
     5161 cggcgagggt tcgttcatcg gcaagggcat ctacgacgtc gacgccttcg agcgcgcggc
     5221 cggctcgatg cccgagaacc gcatcctcag ccacgacctg atcgagggct gctacgcgcg
     5281 ctcgggcctg ctgagcgacg tccagctgat cgaggacgtt ccgtcccgct acgccgtcga
     5341 catggcccgc cgccaccgct gggtgcgcgg cgactggcag ctgatcggct ggctgcgccg
     5401 gcgcctgcac ctgctgcccg gcaagccgcg caacccgctg tccgcgctct cgcgctggaa
     5461 gctgctcgac aacctgcgcc gcagcctcgt gccgaccgcg ctggtcgcgc tggcggtggt
     5521 gggctgggcg tggcttcccg agccgctcat gtggacgctg cgcgtcgtcg tgatcgtcgg
     5581 catcgtgccc ttcgccgcgc acgtgatggg cctgctgcgc acgccgctgg actggatggc
     5641 ctcgtcccac tcgcccgcac gcctgcagcc gatcggccgc cagctgctgc agatggtgca
     5701 ctcgctcgcc tgcctgccga cggaggccgc cgccacgctg gacgcgatcg gccgcgcgct
     5761 gtggcgcatg gtgcgcggac ggcggctgct cgagtggacg gcgtcctcgg acatcccgcg
     5821 cggctcggcg cccggttcgc tggccgcgct gcgcgacagc atccgcgcgc tgtgggcggg
     5881 cccggtcgcc gcgatcgcga tcgccgcgct actcgcgggc gtgcggccgt gggcgcttcc
     5941 ggcggcggcc cccctgctgc tgctgtggtt gtggtcgccg ctgatcgtct ggtgggccga
     6001 ccggccgctc gccgagccgc gcagcgagct cgacgccggc cagcgccact acctgcgccg
     6061 cgtcgcgcgc cgcacgtggg cattcttcga gaccttcgtg ggcgccgacg acaaccacct
     6121 gccgcccgac aacgtgcagc tccaccccgt cgcgcgcgtc gcgcaccgca cctcgccgac
     6181 caacatcggc ttctcgctgc tggccaatct caccgcgcgc gacctcggct acgccacgct
     6241 cgaccaggtg ctgacgcgca tcgcggcgac cgtgacgacg ctggagcgcc tcgagcgcca
     6301 ccgcgggcac ttctacaact ggtacgacac gcagtcgctg ctgccgctcg cgccgcgcta
     6361 catctccacg gtggactcgg gcaacctcgt cgcacagctg atgacgctgc gcatggcgct
     6421 cctggcgatg cccgccgagg cgcccttctc gcccaactgg tgccacgggc tggccgatct
     6481 cgtcggcctg gtgcgcgaga cgctgcccgc gggcgcgtcc gtggggctgg tcgacaagct
     6541 ggaccgccag ctcgccgcgc tgtgcgtggt cccgccgacc accgcggcca ccctgaagtc
     6601 catgctcgag accgtggacg gcctcgcgcg ccagctggtc gccgcggtgg aatcggcgct
     6661 gtccggcgcg gccgcgcggg gcgagcatcc acccgacgcc gccgaggacc acaacggcga
     6721 ggcgctgcgc tgggcgcagc tgctcgtcgc ccagtgcgag gccgggctcg acgagttcaa
     6781 cgcgctcggc ggcctcgcgc cgccgctgcc cgacgacgcg acccacgccg cccaggaaga
     6841 ggcccgcaag gcgccgatcc cgagcctcgc ggcgctcgcg ggcgcgggcg tcggcaccgc
     6901 cgcggtctac ctcgaccgca tcgccgagct cgcccgccgc gtcgacgcga tcaccgagat
     6961 ggagcagcag ttcctctacg acgaccgccg ccacctgatg acgatcggct acaacgtcga
     7021 cgagcggcgc ctggacgcga gccactacga cctgctggcc tcggaggcgc ggctgggcat
     7081 cttcgccgcc atcgcgcgcg gccagctgcc gcaggagagc tggttcgcgc tcggccggct
     7141 gctgacctcc gtcgacggcg acccggtgct gatgtcgtgg agcggctcga tgttcgagta
     7201 cctgatgccg ccgctggtca tgccggagtt cgagcacacg ctgctcgcgc agaccagccg
     7261 cgccgcggtg cgccgccaga tcgagtacgg ccgcgagcgc ggcgtgccct ggggcatctc
     7321 cgagtcgggc tacaacgcga ccgacacggc gctcaactac cagtaccgcg ccttcggcgt
     7381 gcccggcctc ggcctgaagc gcggactcgg cgacgagctc gtgatcgcgc cctacgccac
     7441 cgccatggcg acgatggtcg agcccgtggc cgcgtgcgcg aacctgcagc gcctgcagac
     7501 gctcggcgcc gcgggcgagt acggcttcca cgaggcggtc gactacacgc ccatgcgcgt
     7561 gccgcgcggg cagacgaagt cggtcgtgcg ctcgttcatg gcgcaccacc agggcatgag
     7621 cctgctggcc atcgagcagg cgctgggcac ctcgcgcatg caggcgcact tcgcctccga
     7681 cccgcgcgtg caggcgacgc tcaccctgct gcacgagcgc gtgcccaagg acgtcgtccc
     7741 ggtcgtgccg gagccgacga tggagccgtc ggcgccgcgc tcggtctcca ccgccaccga
     7801 gaccgcgctg cgcgtcttca ccgacccgca gacccccacg cccgaggtgc agctgctgtc
     7861 caacggcagc taccacgtga tggtcacgca gtcgggcgcc ggctacagcc gctggcgcga
     7921 cctggccgtc acgcgctggc gcgaggacgc gacgcgcgac gactggggca gcttctgcta
     7981 cctgcgcgac gtcgacaccg gcgaattctg gtccaccgcc taccagccca ccgtgcgcaa
     8041 gcccgaggac taccaggcca tcttcaccga gggtcgcgcc gagttccgcc gccgcgacgc
     8101 cggcctggac acgcacgtcg agatcgccgt ctcgcccgag gacgcgatcg agctgcgccg
     8161 cgtgcggctg aagaacggga cgcgccggcg ccgccgcatc gagatcacga gctactgcga
     8221 ggtcgtgctg atgacgcccg cggccgacgt gcagcacccc gcgttcggca agctgttcgt
     8281 gcagaccgaa ctggtcgacg gcgtgcccgc gctgctcgcc aaccggaggc cgcgcgcgga
     8341 gaaggatccg tcgccctgga tgttccacct gatggcggtg cacacgccca acgggccgga
     8401 caccgtctcg ccggtgacgc acgagaccga ccgcgcgcgc ttcatcggcc gcggccagac
     8461 gctgcgcgca ccggccgcgc tgctgggcgc cgacgcgctg tccaacacgc agggctccgt
     8521 gctggacccg tgcgcggcca gccgttgcac cgtcgtgctg gagcccgacc agacggtgac
     8581 ggtggatctc gtgatcggcg tcgccgacaa gcgcgaggac tgcctcggcc tgatcgagaa
     8641 gtaccgcgac cgccgcctgg ccgagcgcgt gttcgagctc gcctggacgc acagccaggt
     8701 gctgctgcgc cagctcaacg cgagcgagag cgaggcgcag atgttcgcgc gcctggcggg
     8761 cgccgtcctc ttcagccagg gcgcgctgcg cgccgacccc gccgtcatcc gccagaaccg
     8821 gcgcggccag tcggggctgt ggggctacgc gatctcgggc gacctgccca tcgtcctggt
     8881 gcagatctcg gacgccgcca acctggagct cgtgcgccag ctcgtcgtgg cccatgcctg
     8941 gtggcgcctg aaggggctgg ccgtcgacct cgtgatctgg aacgaggagc gcgacgtcta
     9001 ccgccagcgc ctgaacgaac aggtcatggg cctgatcgcc gcgggcgtcg aggcccacgt
     9061 cgtcgatcgc ccgggcggca tcttcgtgcg ccaggccgac cagatcccgc acgaggatcg
     9121 cgtgctgctg ctgtcggtgg cgcgcgccgt gctgagcgac cgccagggca cgctcgccga
     9181 gcagctcgcg cgccgcctgc gcacggagcg ccgcaccacg ccgcgcccgc tgcgccc
//
LOCUS       DASVLN010000729         2651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_522590, whole genome shotgun sequence.
ACCESSION   DASVLN010000729 DASVLN010000000
VERSION     DASVLN010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522590"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1268)
                     /locus_tag="VF453_17270"
     CDS             complement(249..1268)
                     /locus_tag="VF453_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7689464.1"
                     /translation="MLSSKLLAGLLAAAALAASLPARADDHVVFATNWRAQAAHGGFY
                     QALVDGTYKRYGLDVEIRQGGPQVNNRPLLPAGRIDFLMTGNLLHSFDNVKNGVPVEV
                     VASMFQKDPQALIAHPGAAKKFEDLAKLPVAYIAKDAQFSWWQWLKADYGFRDEALRP
                     YNYNVAPFLADPKSIQQGYAVEEPITIEQQGHFKPDVYLLADHGYSTYSTTIEARVDT
                     VKNKPDLVRRFVEASIIGWNNYLYGDRRAADAAMIKDNPEMTPELIAKSLALMKQMGI
                     VDSGDAQTLGIGALKPERVKDFYDKMVRAGLYKPGEVDLSKVATYEFVNKGVGVELRK
                     KLTGH"
     gene            1381..>2651
                     /locus_tag="VF453_17275"
     CDS             1381..>2651
                     /locus_tag="VF453_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7689465.1"
                     /translation="MTPTTSPATATFSPHPDTDLTTLANGVRIVTLRMPWLASASASV
                     FVRTGSGHESAALNGISHVVEHMAFKGTTTRDAARINLDAELLGAEVNAHTDKDHTAY
                     HLRGMARDAGAFVRMLGDIVRRPTFPAVELERERQVILQEFLEDEEDPMSTAFRQFDK
                     LCYGAHPMAQAVIGTRRNIERFTRDELLAYVDAQYTGANVVIGVAGNVDHAQIVREAE
                     AGFGDLRAGAPQPVPAPGWHGGVKAARLAGCSQSHVVLGYPIASMQDDQPAASVAAAL
                     FGEGMSSPLMHEIRERRGLVYYAACSADIMESCGQFVIEASTTPDKVEDLLREAYRLL
                     VAHAESIAPVDLQRARNQIAVRRMRAQERPVRRVEDAALDLFVLGRVRSPAELRERIE
                     SVTADQVRETFARMLAAPPALSLAGKLGALTG"
BASE COUNT          364 a          917 c          978 g          392 t
ORIGIN      
        1 gggcgcgacg tggcgcccgg gcaagggaac gctgctcgaa ctcgcggaac gtgccggcct
       61 gcagccggac tggggctgcc gcgagggctc gtgagggagc tgccgcacac gcgtggcggc
      121 cggcgccgtc gcctacccgg accgggtgcc gcccgcgggg ccggcggatt cggcgctgat
      181 ctgctgcgcg gtgccggccg ccggcgcgga cgggctggca ctcgacctgt gaggcgccgc
      241 gtccgcgctc aatggcccgt cagcttcttg cgcagctcca cgccgacgcc cttgttgacg
      301 aactcgtagg tcgcgacctt ggacaggtcg acctcgcccg gcttgtacag gcccgcgcgc
      361 accatcttgt cgtagaagtc cttgacgcgc tcgggcttga gcgcgccgat gcccagcgtc
      421 tgcgcgtcgc cggagtcgac gatgcccatc tgcttcatca gcgccaggct cttggcgatg
      481 agctcgggcg tcatctcggg gttgtccttg atcatcgccg cgtcggccgc gcggcggtcg
      541 ccgtagaggt agttgttcca gccgatgatc gaggcctcga cgaagcggcg caccaggtcg
      601 ggcttgttct tcaccgtgtc gacgcgcgcc tcgatggtcg tcgagtacgt gctgtagccg
      661 tggtcggcga gcaggtagac gtcgggcttg aagtggccct gctgctcgat cgtgatcggc
      721 tcctcgacgg cgtagccctg ctggatcgat ttcgggtcgg cgaggaaggg cgcgacgttg
      781 tagttgtacg ggcgcagcgc ctcgtcgcgg aagccgtagt cggccttgag ccactgccac
      841 cacgagaact gggcgtcctt ggcgatgtag gcgaccggca gcttcgcgag gtcctcgaac
      901 ttcttcgcgg cgccgggatg ggcgatcagc gcctggggat ccttctggaa catcgaggcg
      961 acgacttcca ccggcacgcc gttcttgacg ttgtcgaagc tgtgcagcag gttgccggtc
     1021 atcaggaagt cgatgcggcc ggccggcagg agcgggcggt tgttgacctg cgggccgccc
     1081 tggcggatct cgacgtcgag gccgtagcgc ttgtacgtgc cgtcgacgag cgcctggtag
     1141 aagccgccgt gggcggcctg ggcgcgccag ttggtggcga agacgacgtg gtcgtcggcc
     1201 cgcgcgggca ggctggcggc gagcgcggcc gcggcgagga gcccggcgag cagcttggag
     1261 gagagcatcg atcgttcctt gtcggcaggg gacgcgcggc atcatacggt cgagcgcggg
     1321 gcggggaaac caagcccttg gcgcggcgcg ggcgggcgcg cggcggggga caatcggacg
     1381 atgaccccga cgacgtcccc ggccaccgcg accttttcgc cgcatcccga caccgacctc
     1441 acgacgctgg ccaacggcgt gcgcatcgtc accctgcgca tgccctggct cgcgagcgcc
     1501 agcgccagcg tcttcgtgcg caccggcagc ggccacgaga gcgcggccct gaacggcatc
     1561 agccacgtcg tcgagcacat ggccttcaag ggcacgacca cccgcgacgc ggcgcgcatc
     1621 aacctcgacg cggagctgct cggcgccgag gtcaacgccc acaccgacaa ggaccacacg
     1681 gcctaccacc tgcgcggcat ggcgcgcgac gcgggcgcct tcgtgcgcat gctgggcgac
     1741 atcgtgcgcc ggccgacctt cccggcggtc gagctcgagc gcgagcgcca ggtgatcctg
     1801 caggagttcc tcgaggacga ggaggatccg atgtccacgg cgttccggca gttcgacaag
     1861 ctgtgctacg gcgcgcaccc gatggcgcag gcggtgatcg gcacgcgccg caacatcgag
     1921 cgcttcacgc gcgacgagct gctcgcctac gtcgacgccc agtacacggg cgcgaacgtc
     1981 gtgatcggcg tcgcgggcaa cgtcgatcac gcgcagatcg tgcgcgaggc ggaggcgggc
     2041 ttcggcgacc tgcgcgccgg cgcgccgcag cccgtgcccg cgcccgggtg gcacggcggc
     2101 gtgaaggccg cgcggctggc gggctgcagc cagagccatg tcgtcctcgg ctatccgatc
     2161 gcgtcgatgc aggacgacca acccgccgcc agcgtcgccg cggcgctgtt cggcgagggc
     2221 atgagctcgc cgctgatgca cgagatccgc gagcgccgcg gcctcgtcta ctacgccgcg
     2281 tgctcggccg acatcatgga gtcctgcggc cagttcgtga tcgaggcgtc gacgacgccc
     2341 gacaaggtcg aggacctgct gcgcgaggcg taccggctac tcgtcgcgca tgcggagtcc
     2401 atcgcgccgg tcgacctgca gcgtgcgcgc aaccagatcg cggtgcgccg catgcgcgcg
     2461 caggagcgcc cggtgcggcg cgtcgaggac gcggcgctcg acctgttcgt cctcggccgc
     2521 gtgcgctcgc ccgcggagct gcgcgagcgc atcgagtccg tcaccgccga ccaggtgcgc
     2581 gagaccttcg cccgcatgct cgccgcgccg ccggcgctgt cgctggccgg caagctgggc
     2641 gcgctgacgg g
//
LOCUS       DASVLN010000730         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_252862, whole genome shotgun sequence.
ACCESSION   DASVLN010000730 DASVLN010000000
VERSION     DASVLN010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252862"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /locus_tag="VF453_17280"
     CDS             complement(<1..1809)
                     /locus_tag="VF453_17280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX7689466.1"
                     /translation="MAVPLTGQTRAGDAHDDARTERDAPAAGTAPGIGLRDGSDKVDI
                     LVVDDLPEKLLVFETVLGELGQNLVFARSGADALRQILRRDFAVVLLDVNMPDMDGFE
                     TAALIRRYKRAAHTPIIFITSYADEMQTARGYSLGAVDYIPSPVVPEVLRSKVRVFVE
                     LHVLQRRIARQAEERIALMASEAALRLAEESTRRSTSLADLSHALSGVLEPGQAVRAL
                     LAHVVPALAAAATVALVDERGVVAHVAARSSPLDGQVVDDELAPSALPTAHFQLLRRL
                     LELSPEERGRGPAESDGALRMLPLIHGERLLGALWIDGEVTPLGDALLDEVAARGAIA
                     FATAALYRNLQVEIAERRQAEARLEESSRRKDEFLAMLSHELRNPLAPIRNAVEVIRL
                     VAPPEQKLRWAADVTDRQVRQLTRLVDELLDVARISQGKIVLQPQCVDLVALVSQCVE
                     THRAFMASRGQTLTQALPGTPVHLNGDATRLAQVINNLLTNATKYTPDNGAISVSVDR
                     ETTPEGDFAVVTVSDDGIGIEADLLPHVFELFEQGKRALDRTQGGLGVGLTLVQRLVR
                     LHGGDVEAESTGTGQGSRFRVRLPCLSPLEDAAPADA"
     gene            complement(1794..>3085)
                     /locus_tag="VF453_17285"
     CDS             complement(1794..>3085)
                     /locus_tag="VF453_17285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689467.1"
                     /translation="LPAPPAPASEPAMDWRDEEDAGKASDAVMFANAADDDRGDISTG
                     DRVLLIVENDLAFARILLAAARRAGFKGVVTGHGASALTMTREYHPAMVTLDIHLPDM
                     SGWRILDRLKGDLATRHVPITVVSTDDARDRALASGALAFLAKPLQSADVAEEFVAQL
                     HDYAERSGRRLAVVMQPSARRQALLASLDGDVEVLLAEDRAAALHALAQADVAVVEDT
                     LPDFGPEDVLDAIGERRGVCQLPVILARGAPVETGSWNQRRPGVAIVEAADLAELRTQ
                     LSLWLHRSPAALSDEDRATIERTLARHQSLKGKKALIVDDDMRNIFALATVLDEEGMV
                     IVSADNGREAIRLVEADPAIDIVLMDIMMPEMDGIATMQAIRQRPRGRELPMVAVTAK
                     AMKGDRQKCIEAGAWDYISKPVDPRHLMAVLRGWLCR"
BASE COUNT          406 a         1140 c         1108 g          431 t
ORIGIN      
        1 cgcgtccgcg ggcgcggcgt cctccagcgg cgacaggcac ggcagccgca cccggaaccg
       61 cgatccctgc cccgtgccgg tgctctcggc ctcgacgtcg ccgccatgca gccgcaccag
      121 ccgctgcacc agcgtgaggc cgacgcccag gccgccctgc gtgcggtcga gggcgcgctt
      181 gccctgctcg aacagctcga agacgtgcgg cagcaggtcg gcctcgatgc cgatgccgtc
      241 gtcgctgacg gtgaccaccg cgaagtcgcc ctcgggcgtc gtctcgcggt cgaccgacac
      301 cgagatcgcg ccgttgtccg gcgtgtactt ggtcgcgttg gtcagcaggt tgttgatgac
      361 ctgcgccagc cgcgtggcgt cgccgttgag gtgcaccggc gtgccgggca gcgcctgcgt
      421 cagcgtctgg ccgcgcgagg ccatgaaggc gcggtgcgtc tcgacgcact gcgagacgag
      481 cgcgaccagg tcgacgcact gcggctgcag cacgatcttg ccctggctga tgcgcgccac
      541 gtccagcagc tcgtcgacca ggcgcgtgag ctggcgcacc tggcgatccg tgacgtcggc
      601 cgcccagcgc agcttctgct ccggcggcgc gaccaggcgg atcacctcca cggcgttgcg
      661 gatcggcgcg agcgggttgc gcagctcgtg cgagagcatc gcgaggaatt cgtccttgcg
      721 gcggctgctc tcctcgagcc gtgcctccgc ctggcggcgc tcggcgatct cgacctgcag
      781 gttgcggtac agcgccgccg tcgcgaaggc gatcgccccg cgcgccgcca cctcgtccag
      841 cagcgcgtcg cccagcgggg tcacttcgcc gtcgatccag agcgcgccga gaagccgctc
      901 gccgtggatc agcggcagca tgcgcagcgc gccgtcgctc tcggcgggcc cgcggccgcg
      961 ctcctcgggc gaaagctcca gcagccggcg cagcagctgg aagtgcgcgg tgggcagcgc
     1021 ggacggcgcg agctcgtcgt cgacgacctg cccgtccagc gggctcgagc gcgcggccac
     1081 gtgcgcgacg acgccccgct cgtcgaccag cgccaccgtc gccgcggccg cgagcgcggg
     1141 caccacgtgc gcgagcagcg cgcgcacggc ctgccccggc tcgagcacgc cgctgagcgc
     1201 gtggctcagg tcggcgagcg aggtcgagcg ccgcgtcgat tcctcggcca gtcgcaaggc
     1261 ggcctcggac gccatcagcg cgatgcgctc ctcggcctgg cgcgcgatgc gccgctgcag
     1321 aacgtgcagc tcgacgaaga cgcgcacctt ggagcgcagc acctccggca ccacgggcga
     1381 cgggatgtag tcgaccgcgc ccagcgagta gccgcgcgcg gtctgcatct cgtcggcata
     1441 cgacgtgatg aagatgatcg gcgtatgcgc ggcgcgcttg tagcggcgga tcagcgccgc
     1501 ggtctcgaag ccgtccatgt cgggcatgtt gacgtcgagc agcacgacgg cgaagtcgcg
     1561 cctgaggatc tgccgcagtg cgtccgcgcc cgagcgcgcg aacacgaggt tctgccccag
     1621 ctcgcccagc acggtctcga agacgagcag cttctccggc aggtcgtcga cgacgaggat
     1681 gtcgaccttg tcggacccgt cgcgaaggcc gatgcccggc gccgtacccg cggcgggtgc
     1741 gtcccgctcg gtgcgggcat cgtcgtgcgc gtcgccggcg cgcgtctggc cggtcagcgg
     1801 cacagccatc ccctcagcac cgccatcagg tggcgcggat cgaccggctt ggagatgtag
     1861 tcccacgccc cggcctcgat gcacttctgg cggtcgccct tcatcgcctt ggccgtcacc
     1921 gccaccatcg gcagctcgcg gccgcgcggg cgctggcgga tcgcctgcat ggtcgcgatg
     1981 ccgtccatct ccggcatcat gatgtccatc agcacgatgt cgatcgccgg atcggcctcg
     2041 accagccgga tcgcctcgcg cccgttgtcg gccgagacga tgaccatgcc ctcctcgtcc
     2101 agcaccgtcg cgagcgcgaa gatgttgcgc atgtcgtcgt cgacgatcag cgccttcttg
     2161 cccttgagcg actggtggcg ggcgagcgtg cgctcgatcg tcgcgcgatc ctcgtccgac
     2221 agcgccgcgg ggctgcggtg cagccacagc gacagctgcg tgcgcagctc ggcgaggtcg
     2281 gccgcctcga cgatcgcgac gcccggccgg cgctggttcc agctgcccgt ctccaccggc
     2341 gccccgcgcg cgaggatcac cggcagctgg cacacgccgc ggcgctcgcc gatcgcgtcg
     2401 aggacgtcct cggggccgaa gtccggcagc gtgtcctcga cgacggcgac gtcggcctgg
     2461 gcaagcgcgt gcagggccgc ggcgcgatcc tcggccagca gcacctcgac atccccgtcc
     2521 agcgaggcga gcagcgcctg gcggcgtgcc gacggctgca tcaccacggc cagccggcgg
     2581 ccggaacgct cggcgtagtc gtggagctgc gcgacgaact cctcggcgac gtcggccgac
     2641 tgcagcggct tggcgaggaa ggccagcgcg cccgaggcga gcgcgcggtc gcgtgcatcg
     2701 tcggtggaga ccacggtgat cggcacgtgg cgcgtcgcga ggtcgccctt caggcggtcg
     2761 aggatgcgcc agcccgacat gtcgggcagg tggatgtcca gcgtgaccat cgccggatgg
     2821 tactcgcgcg tcatcgtcag cgcgctcgcg ccgtgcccgg tgaccacgcc cttgaatccc
     2881 gcgcggcgtg cagcggccag caggatgcgc gcgaacgcga ggtcgttctc gacgatcagc
     2941 agcacgcgat caccggtgga gatgtcgccg cggtcgtcgt cggccgcgtt ggcgaacatg
     3001 acggcgtcgc tcgccttgcc ggcatcctcc tcgtcgcgcc agtccatggc cggctccgaa
     3061 gccggcgccg gcggcgcggg cagcc
//
LOCUS       DASVLN010000731         7708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_360009, whole genome shotgun sequence.
ACCESSION   DASVLN010000731 DASVLN010000000
VERSION     DASVLN010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360009"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VF453_17290"
     CDS             <1..264
                     /locus_tag="VF453_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7689468.1"
                     /translation="AMLRAFLAGAAAHLAPGGEAWLVMSDLAEHLGLRTRAELEGWIA
                     AGGLRVVARHDTRPVHPKAVDASDPLHAARSRETTSLWRLAAP"
     gene            320..886
                     /locus_tag="VF453_17295"
     CDS             320..886
                     /locus_tag="VF453_17295"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7689469.1"
                     /translation="MIAGDFPVLETPRLRLREIVAADAPALLEIHGDPVVMRWFGNDP
                     IADLAGAHRIVENFAGLRWLPSPGTRWAIEPRDSPDGELVGTCGFFRWNRGWRTCMVG
                     YELAPRAQGRGYMREALRAIVPWAFATLGIERIEAQVHPDNAASLATLAALGFRREGL
                     QRAAAFWGGRRHDMVVMGLLREDFRATD"
     gene            complement(902..1291)
                     /locus_tag="VF453_17300"
     CDS             complement(902..1291)
                     /locus_tag="VF453_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7689470.1"
                     /translation="MLLRLHHAAIICSDYEASKRFYAEVLGLRVLAEHWRAARRSWKC
                     DLALPDGSQVELFSFEGAPPRPSYPEARGLRHLAFAVADVAAFKAGLEARGVAVEAIR
                     VDEYTGRRFTFFADPDDLPLEVYEVEA"
     gene            complement(1304..2719)
                     /locus_tag="VF453_17305"
     CDS             complement(1304..2719)
                     /locus_tag="VF453_17305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7689471.1"
                     /translation="MTGRAGAPAAGAARPPLYRQLALRLMIPLMLIVAAVGAMGVWGA
                     EAETAAVFDRWLLDAAVSLADQVRPVRGDGPHAVDLPEAARAMLAFDEVDRTWFAVED
                     RGRLLVGSAGIPGSGAGGTAYPPWGWAFDATYQGRPVRVAAVAARCEGCERVVVLVAE
                     TRIKRERARRVARWLLLPLALMLAVTCGAIFLAIRRTVQPLEGIATQWQRQSHASLRA
                     IPADDLPRELTPFAVALNDLIARIRELLVRERTFAAAAAHQLRTPLTALRLGLARART
                     SPDLESTRAVIDELARITETSGRLVQQLMLLGRLDPEGRADVDRVPVDLRDIARDVCQ
                     LFAEVALEQEIDLELRAADAPVMVDVQPDLLVEALANLIDNAIKAAGPRGQVLVTVQS
                     RPARVRVNDSGPGIRPHERRTIFERYVRGSAASWSGTGLGLAIVHDIARLQHARVAVV
                     DGELRGACFEFAFDDPPAADA"
     gene            complement(2716..3426)
                     /locus_tag="VF453_17310"
     CDS             complement(2716..3426)
                     /locus_tag="VF453_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7689472.1"
                     /translation="MRLLLIEDDPDIARELILRWRESDRLARHAPSLAQADAALAEET
                     FDIVLLDLGLPDGDGMEWLAEYRKRDPHGAVLVTTARDRVADRVQGLRVGADDYLVKP
                     FAPEELDARIDVLVRRANLSRSRSIRFGRLDLLPDDQEVLCDGRRLEVSPREFEVLFI
                     LMRAAPRLVSKRALVDALSERNLDLNDAAAELYVSRLRKKLDGTGTGIRTLRGVGYQL
                     AIVAPAAAPPVGDVAARE"
     gene            complement(3640..4845)
                     /locus_tag="VF453_17315"
     CDS             complement(3640..4845)
                     /locus_tag="VF453_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX7689473.1"
                     /translation="MTIDPGIKLLIVDDLPENLLALEASIRDDRYSIHQASSGEEALA
                     LLLEHEFALAILDVQMPGMNGFELAELIRGMARTRHLPIVFVSAAGKELDYAFKGYEN
                     GAVDFLYKPLDAYAVKSKVQVFVDLYHQRTELRRQLAALEESRRRQQALVAELQTAQV
                     DLQRAVKMRDEFMSMVVHELRTPLSVLAMEVRVRQHQVERGNMEFFAPDKLAKMLERD
                     QRQVRSLTRLIDDMLDVSQIQHGKLSIRRGRCDLTAMLRRVVAEVQGQRGDVEILLDP
                     ECVEISGEWDEFRLEQVVVNLLTNALRYGAGKPVHVSLRQRPGAAVIQVRDEGVGIAP
                     EDLQRVFEQFVRVGERTRVPGLGLGLYITRQLVEAHGGTIAVESTLGEGSTFTVELPT
                     AGGTGGERP"
     gene            complement(4842..5456)
                     /locus_tag="VF453_17320"
     CDS             complement(4842..5456)
                     /locus_tag="VF453_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX7689474.1"
                     /translation="MTAIDRDDALRAALARHIEAVVIGASAGGFEALMAVLGGLPADY
                     PSPVSIVLHLPDNRDSRLAELFGYRLAMDVREARDKEPLAPGTVYFAPPGYHLLIEKD
                     RSFSLSCEDRVNYARPSIDVLMLSAADAYGAGLCGILLTGANYDGAEGLAGMQIAGGL
                     AVVQDPATAEVPTMPEAALRRMTPDLVLSLAEIHDLLRRLGAPR"
     gene            complement(5563..6381)
                     /locus_tag="VF453_17325"
     CDS             complement(5563..6381)
                     /locus_tag="VF453_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEX7689475.1"
                     /translation="MSHSAIDIELRLLMEAVYAKYSHDFRDYSGASMKRRVLHAMQQL
                     QCGTVSELQSRVLHDPGAFQQLLEILTIPFTEMFRDPGYWLALRTQVVPLLRTYPSLK
                     LWVAGCSTGEEVFSLAILLQEEGLLERSMIYATDINPAVLEKARQGIYAVGAVRQHTA
                     NYQASGGKRAFSDYYAAAYKSVRFDPELVRNVTFADHSLATDTVFSETHLISCRNVLI
                     YFNRALQNRALGLFHDSLCHRGFLGLGSKESLDFSAWADRFDAVDRRERLFRKR"
     gene            complement(6430..>7708)
                     /locus_tag="VF453_17330"
     CDS             complement(6430..>7708)
                     /locus_tag="VF453_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689476.1"
                     /translation="IPPLPAPVAAEAGATLPPAGAIKDDRDQVRPGDKGRLLLVVEDD
                     TDFANVLMLLARERRYQCLVATTASEALALATGYLPTAILLDVRLPDGSGLSVLQFLK
                     DDARTRHIPVHIVSGEDFSDVALHLGAVGVAVKPTTREELQQVFQRLEEKGAKKVKRV
                     LVVEDDARQRDSVVHLIEDDDIEIAAVEDGAKALALLKQTVYDCMIIDLKLPDMDGSE
                     LLERMTTEDICSFPPVIVYTGRNLTREEEARLGRYSRSIIIKGARSPERLLDEVTLFL
                     HTVESQLSQERQSMLRTARNREKVFEGRKVLLVDDDVRNIFALASALEQKGLQVEIGR
                     NGFEALARLAEVPDIDIVLMDVMMPGMDGLEATRRIRANPKWAQLPVIAVTAKAMKDD
                     QEQCRRAGANDYLAKPIDLDRLFSLMRVWMPSI"
BASE COUNT         1083 a         2748 c         2796 g         1081 t
ORIGIN      
        1 gcgatgctgc gcgccttcct cgcgggcgcg gccgcgcacc tcgcgccggg cggcgaggcg
       61 tggctggtga tgtccgacct cgccgagcac ctcggcctgc gcacccgcgc cgagctcgaa
      121 ggctggatcg ccgcgggcgg cctgcgcgtg gtcgcgcgcc acgacacgcg ccccgtccat
      181 ccgaaggcgg ttgacgcgtc cgatccactg cacgccgcgc gctcgcgcga gacgacgtcg
      241 ctctggcgcc tggccgcgcc gtgacgaccg ccccgaacct cgcccgccgt catcggaagg
      301 cggccagaaa ggagttcgca tgatcgccgg cgacttcccc gtcctcgaga ccccgcgcct
      361 gcgcctgcgc gagatcgtcg ccgccgacgc gccggcgctg ctggagatcc acggcgaccc
      421 ggtcgtgatg cgctggttcg gcaacgaccc gatcgccgac ctcgcgggcg cgcaccgcat
      481 cgtcgagaac ttcgccggcc tgcgctggct gccgagcccc ggcacgcgct gggcgatcga
      541 gccgagggac tcgcccgacg gcgagctggt gggcacctgc ggcttcttcc gctggaaccg
      601 cggctggcgc acgtgcatgg tcggctacga gctcgccccg cgcgcgcagg gccgcggcta
      661 catgcgcgag gcgctgcgcg caatcgtccc gtgggccttc gcgacgctcg ggatcgagcg
      721 catcgaggcc caggtgcatc ccgacaacgc cgcctccctc gcgacgctgg ccgcgctcgg
      781 tttccggcgc gagggcctgc agcgcgccgc cgccttctgg ggcggtcgac gccacgacat
      841 ggtggtgatg gggctgctgc gcgaggactt ccgcgcgacg gattgacgcg agcgccggcc
      901 tctaggcctc gacctcgtag acctcgagcg gcaggtcgtc cggatcggcg aagaaggtga
      961 agcggcggcc cgtgtactcg tcgacgcgta tggcctcgac cgcgacgccg cgggcctcga
     1021 ggccggcctt gaacgcggcg acgtcggcga ccgcgaaggc gagatggcgc aggccgcggg
     1081 cctcgggata ggacgggcgc ggcggcgcgc cctcgaacga gaacagctcg acctgcgagc
     1141 cgtccggcag cgcgaggtcg cacttccagg agcggcgcgc cgcgcgccag tgctcggcga
     1201 gcacgcgcag gcccagcacc tcggcgtaga agcgcttcga ggcctcgtag tcggaacaga
     1261 tgatcgcggc gtggtgcagg cgcaggagca tgccgccatt gtctcaggcg tccgccgcgg
     1321 gcgggtcgtc gaacgcgaac tcgaagcagg cgccccgcag ctcgccgtcg accaccgcga
     1381 cgcgcgcgtg ctgcaaccgc gcgatgtcgt gcacgatcgc caggcccagg cccgtgcccg
     1441 accagctcgc cgcgctgccg cgcacgtagc gctcgaagat cgtccggcgc tcgtgcggcc
     1501 ggatgcccgg gccgctgtcg ttcacgcgca cgcgcgccgg gcgggactgc acggtcacga
     1561 gcacctggcc gcgcgggccg gcggccttga tcgcgttgtc gatcaggttg gcgagcgcct
     1621 cgaccagcag gtccggctgc acgtcgacca tcaccggcgc gtcggctgcg cgcagctcga
     1681 ggtcgatctc ctgctccagc gcgacctccg cgaacagctg gcagacgtcg cgcgcgatgt
     1741 cgcgcaggtc gaccggcacg cggtcgacgt cggcgcgacc ttcgggatcg aggcgcccga
     1801 gcagcatcag ctgctgcacg agccggcccg aggtctcggt gatgcgggcg agctcgtcga
     1861 tcaccgcgcg cgtgctctcg aggtcggggc tcgtgcgcgc acgcgcgagg cccaggcgca
     1921 gcgcggtgag cggcgtgcgc agctggtggg ccgcggccgc cgcgaaggtg cgctcgcgca
     1981 ccagcagctc gcggatgcgc gcgatcaggt cgttgagcgc gacggcgaac ggcgtgagct
     2041 cgcgcggcag gtcgtcggcc gggatggcgc gcagcgatgc gtgcgactgg cgctgccatt
     2101 gcgtcgcgat gccctccaga ggctgcaccg tgcggcggat cgccaggaag atcgccccgc
     2161 acgtcaccgc aagcatcagc gcgagcggca gcagcagcca gcgcgcgacc cgccgcgcgc
     2221 gctcgcgctt gatccgggtc tcggcgacga gcaccacgac gcgctcgcag ccctcgcagc
     2281 gcgcggccac cgccgcgacg cgcacggggc ggccctggta ggtcgcgtcg aaggcccagc
     2341 cccagggcgg gtaggccgtc ccgccggcgc cgctgccggg gatgcctgcg ctgcccacga
     2401 gcaggcggcc gcgatcctcg accgcgaacc aggtgcgatc gacctcgtcg aaggccagca
     2461 tcgcgcgcgc ggcctcgggc aggtcgacgg catgcgggcc gtcgccccgc accggacgga
     2521 cctggtcggc gagcgagacc gcggcatcga gcagccagcg gtcgaagacg gccgcggtct
     2581 ccgcctcggc gccccacacg cccatcgcgc cgaccgcggc gacgatcagc atcagcggga
     2641 tcatcaggcg cagcgccagc tgccggtaga gcggcggacg cgccgccccg gccgcggggg
     2701 cgcccgcgcg gccggtcatt cgcgcgccgc gacgtcgccg acgggcggcg ccgcggcggg
     2761 cgcgacgatc gccagctggt agccgacgcc ccgcagcgtg cggatgccgg tgccggtgcc
     2821 gtcgagcttc ttgcgcaggc gcgagacgta gagctcggcg gcggcgtcgt tcaggtcgag
     2881 gttgcgctcc gacagcgcgt ccaccagcgc gcgcttggac acgagccgcg gcgccgcgcg
     2941 catcaagatg aacagcacct cgaactcgcg cggcgagacc tcgaggcggc ggccgtcgca
     3001 cagcacctcc tggtcgtcgg gcagcaggtc gagccggccg aagcggatcg agcgcgagcg
     3061 cgaaaggttg gcgcgccgca ccagcacgtc gatgcgggcg tcgagctcct cgggcgcgaa
     3121 cggcttgacg aggtagtcgt ccgcgccgac gcgcaggccc tgcacccggt cggccacgcg
     3181 gtcgcgcgcg gtcgtcacga gcacggcgcc gtgcggatcg cgcttgcggt actcggcgag
     3241 ccattccatg ccgtcgccat cgggcagccc gaggtcgagc agcacgatgt cgaaggtctc
     3301 ctccgccagc gccgcgtcgg cctgggccag cgaaggcgca tggcgggcga ggcggtcact
     3361 ctcgcgccag cgcaggatca gctcgcgcgc gatgtcgggg tcgtcttcga tgagcagcag
     3421 tcgcatggca gcgggcgtcg cgcggacgcg ggtcggggtg tcgcatcctg ccgcatccgc
     3481 gccgccgccg ccgcgtgccg gcgcggccgg atgtgcacga atcccgactt taggacagga
     3541 agcgtcggca aaacccctcg cgatcgactt cgacggcgat cgggcgggct cgccagccgg
     3601 ccacgcgcgc gcgtccgccg gaccgaccgg tcgcggcgct cagggccgct cgccgccggt
     3661 gccgccggcc gtgggcaatt ccacggtgaa ggtcgagccc tcgccgagcg tgctctcgac
     3721 ggcgatcgtg ccgccgtggg cctccacgag ctggcgcgtg atgtacaggc ccaggcccag
     3781 gccgggcacg cgcgtgcgct cgcccacgcg cacgaactgc tcgaacaccc gctgcaggtc
     3841 ctcgggcgcg atgcccacgc cctcgtcgcg cacctggatg acggcggcgc caggccgctg
     3901 gcgcagcgac acgtgcacgg gcttgccggc accgtagcgc agcgcgttgg tcagcaggtt
     3961 gacgacgacc tgctcgaggc ggaactcgtc ccactcgccc gagatctcca cgcattccgg
     4021 atcgagcagg atctcgacgt ccccgcgctg gccctgcacc tcggcgacca cgcgccgcag
     4081 catcgcggtg aggtcgcagc gcccgcgccg gatcgacagc ttgccgtgct ggatctgcga
     4141 gacgtccagc atgtcgtcga tcaggcgcgt gaggctgcgc acctgccgct ggtctcgctc
     4201 cagcatcttg gcgagcttgt ccggcgcgaa gaactccatg ttgccgcgct cgacctggtg
     4261 ctggcgcacg cgcacctcca tcgcgagtac gctcagcggc gtgcgcagct cgtggacgac
     4321 catggacatg aactcgtcgc gcatcttcac cgcgcgctgc aggtcgacct gcgcggtctg
     4381 cagctccgcg acgagcgcct gctggcgacg gcgcgattcc tccagcgccg cgagctggcg
     4441 ccgcagctcg gtgcgctggt ggtagaggtc gacgaagacc tgcaccttgc tcttgaccgc
     4501 gtaggcgtcc agcggcttgt agaggaaatc gacggcgccg ttctcgtagc ccttgaacgc
     4561 gtagtcgagc tccttgcccg cggcgctcac gaacacgatc ggcaggtggc gcgtgcgcgc
     4621 catgccgcgg atcagctccg cgagctcgaa gccgttcatg ccgggcatct gcacgtcgag
     4681 gatggcgagc gcgaactcgt gctccagcag cagcgccagc gcctcctcgc ccgaggacgc
     4741 ctggtggatg ctgtagcggt cgtcgcggat ggaggcttcc agcgcgagca ggttctcggg
     4801 caggtcgtcg acgatcagga gcttgatgcc ggggtctatc gtcatctggg tgcgcccagc
     4861 ctgcgaagca ggtcgtggat ctccgcgagt gaaaggacga ggtcgggggt cattcttcgc
     4921 agcgcggctt ccggcatcgt cgggacttcc gcggtcgccg ggtcctggac gacggcgaga
     4981 cccccggcaa tctgcattcc cgcgagaccc tcggcaccgt cgtagttcgc acccgtgagc
     5041 aatatcccac acaacccggc gccgtacgcg tcggcggcgg acagcatcag cacgtcgatc
     5101 gagggccgcg cgtagttgac gcgatcctcg cagctgaggg agaagctgcg atccttctcg
     5161 atcagcaggt ggtagccggg cggtgcgaag tagacggtgc ccggggcgag cggctccttg
     5221 tcgcgcgcct cgcgcacgtc catcgcgagg cggtaaccga acagctccgc gaggcggctg
     5281 tcgcggttgt cggggaggtg cagcacgatg gacactggcg acggatagtc cgccggcagt
     5341 cctccgagca ccgccatcag cgcctcgaag ccgcccgccg aggcgccgat cacgacggcc
     5401 tcgatgtggc gtgccagcgc cgcgcgcagg gcgtcgtcgc gatcgatggc agtcatcacg
     5461 gtcacgccgt gcccggacaa gcggcagccg cgcaaccggc tgcgcggggc tgccggttgc
     5521 ggccgcccgg gggatggcgc cgaaggcgct tcgcaaaggt catcatctct tccggaacag
     5581 gcgttcgcgc cggtcgacgg cgtcgaagcg atcggcccag gccgagaagt ccaggctctc
     5641 cttgctgccg aggccgagga agccgcggtg gcacagcgag tcgtggaaca ggcccagcgc
     5701 gcgattctgc aaggcgcggt tgaagtagat cagcacgttg cggcaggaga tcaggtgcgt
     5761 ctccgagaac accgtgtcgg tggccaggct gtggtcggcg aaggtgacgt tgcgcaccag
     5821 ctcgggatcg aagcgcaccg acttgtacgc cgcggcgtag tagtccgaga acgcgcgctt
     5881 gccgccgctt gcctggtagt tggccgtgtg ctggcgcacc gcgccgaccg cgtagatgcc
     5941 ctggcgcgcc ttctcgagga cggccgggtt gatgtccgtc gcgtagatca tcgagcgctc
     6001 cagcagccct tcctcctgca gcaggatcgc gagcgagaac acctcctcgc cggtgctgca
     6061 gcccgccacc cacagcttca gcgacggata ggtgcgcagc agcggcacga cctgcgtgcg
     6121 cagcgcgagc cagtagcccg ggtcgcggaa catctcggtg aacgggatcg tcaggatctc
     6181 gagcagctgc tggaacgcgc ccgggtcgtg cagcacgcgc gactgcagct ccgagacggt
     6241 gccgcactgc agctgctgca tcgcgtgcag cacgcggcgc ttcatcgacg cgccggagta
     6301 gtcgcggaag tcgtggctgt acttcgcgta gacggcctcc atcagcaggc gcagctcgat
     6361 gtcgatggcg ctgtgagaca tggcggaggt cggcgatcgt cccgcgtcgt gtacggtact
     6421 gggtatccgt cagatgctcg gcatccagac ccgcatcagc gagaacaggc ggtcgaggtc
     6481 gatcggcttg gcgaggtagt cgttggcgcc ggcgcggcgg cactgctcct ggtcgtcctt
     6541 catcgccttg gccgtcaccg cgatcaccgg cagctgcgcc cacttcgggt tcgcgcggat
     6601 gcggcgcgtc gcctcgaggc cgtccatgcc gggcatcatc acgtccatca gcacgatgtc
     6661 gatgtcgggc acctcggcca gccgggcgag cgcctcgaag ccgttgcggc cgatctcgac
     6721 ctgcaggccc ttctgctcca gcgcgctcgc gagcgcgaag atgttgcgca cgtcgtcgtc
     6781 gaccagaagc accttgcggc cctcgaacac cttctcgcgg ttgcgcgcgg tgcgcagcat
     6841 cgactggcgc tcctgcgaca gctgcgattc cacggtgtgc aggaacagcg tcacctcgtc
     6901 gagcaggcgc tccggcgagc gcgcgccctt gatgatgatc gagcgcgagt agcggccgag
     6961 gcgggcctcc tcctcgcgcg tgaggttgcg gccggtgtag acgatcaccg gcgggaacga
     7021 gcagatgtcc tcggtcgtca tgcgctcgag gagctcgctg ccgtccatgt cgggcagctt
     7081 caggtcgatg atcatgcagt cgtacacggt ctgcttgagc aaggcgagtg ccttggcgcc
     7141 gtcctcgacc gccgcgatct cgatgtcgtc gtcctcgatc aggtggacga cgctgtcgcg
     7201 ctggcgcgcg tcgtcctcca cgaccagcac gcgcttgacc ttcttggcgc ccttctcctc
     7261 gaggcgctgg aagacctgct gcagctcctc gcgcgtcgtc ggcttgaccg cgacccccac
     7321 cgcgcccaga tgcagggcga cgtcggagaa gtcctcgccc gagacgatgt gcaccgggat
     7381 gtggcgggtg cgcgcatcgt ccttgaggaa ctgcagcacg ctcaggcccg agccgtcggg
     7441 caggcgcacg tccagcagga tggcggtggg caggtagccg gtcgcgagcg cgagggcctc
     7501 gctggcggtc gtcgcgacca ggcactggta gcgccgctcg cgcgcgagca gcatcagcac
     7561 gttggcgaag tcggtgtcgt cctcgacgac cagcagcagc cggcccttgt cgccgggccg
     7621 cacctggtcg cggtcgtcct tgatcgcgcc ggccggcggc agcgtcgcgc cggcctcggc
     7681 cgcgacgggc gcgggcagcg gcgggatc
//
LOCUS       DASVLN010000732         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_252915, whole genome shotgun sequence.
ACCESSION   DASVLN010000732 DASVLN010000000
VERSION     DASVLN010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252915"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..2247)
                     /locus_tag="VF453_17335"
     CDS             complement(82..2247)
                     /locus_tag="VF453_17335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689477.1"
                     /translation="MNIRIRLMLLVFAVWLPAAVGFGMLARSIFLQQTATGREHLRAY
                     GRTLNLLVERELDRRATMARTLGASLALRRDDLRGFYDEAATAARGGDSWVILVTPTA
                     EILNTHVPWQEGLRIARPPGAPMVTSDAADVFFAPVGPVTHGPVLALFRPELGVAPPR
                     YNVGVAFEPASIQALLAQQPGPEDALVTVMDDEQVIIARTREPRRWLGVSAGPEIKRR
                     ARDEAEGFGESITLDGVRSLTYVSRPNRYHWQVVVALPTQGLSRTARVLTLQAVGAAA
                     ALLVISLGLVVYASRRISGPVGQLHDAAATLREDRVPAPLATGVREVDDISTVLHDAG
                     VHSQEATRTLEQRVANAVQQAEDAQAKLLEGQKHEAIGRLTGGLAHDFNNLLQTISTA
                     LQVFERTKDGPPHPRTLEAAIRATHRAAQLVRQMLTFGRTRPQQLAAVNFADFLLGAR
                     ELVQKAIGQSVELVADVEPGLRHVNVDPSQLELALLNLIFNARDAMPEGGRVTVSARS
                     TGPDRAVIAVTDTGTGMDEQTLARATDPYFTTKPVGRGSGLGLAQVRGFALQSGGDIR
                     LHSAPGRGTTVEMHLPTVDAPASEDAEPEHPHSGRPLRILMVEDDLLVSSVVVPALDA
                     AGHAVTLAATADQAVELLQRGAPFDVVFTDVVMPGRMSGTDLAAWCREHRPATPVVVA
                     TGYSAQALPSVEVLNKPYDLHALLEALDRAAATSGQAGP"
     gene            2261..>2491
                     /locus_tag="VF453_17340"
     CDS             2261..>2491
                     /locus_tag="VF453_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689478.1"
                     /translation="MDSKAWTLSEFAAEVIGARPRLDTVEGMHSTTPLRILLVNNSSA
                     AAFRLVPPPADAVLADAPPAATWRAAAEGQVDA"
BASE COUNT          322 a          907 c          926 g          336 t
ORIGIN      
        1 cgctgcccgc gaggacggcg gcgagccgca acacggcgcg gcgggcgacg ggggcgtccg
       61 ctgaacgacg ctgtcgcgcc ttcatgggcc ggcctgcccg gacgtcgccg cggcccggtc
      121 cagcgcctcc agcagcgcgt gcaggtcgta aggcttgttg aggacttcca cgctcggcaa
      181 cgcctgcgcg gaatagcccg tggccacgac gacgggcgtc gcgggccggt gttcgcggca
      241 ccaggcggcg aggtccgtgc cgctcatgcg gcccggcatc accacgtcgg tgaagacgac
      301 gtcgaagggg gcgccgcgct gcagcagctc gacggcctgg tcggccgtcg ccgcgagcgt
      361 gacggcgtgg ccggcggcgt cgagcgccgg cacgaccacc gacgagacga gcaggtcgtc
      421 ctcgaccatc aggatgcgca gcggccggcc cgaatgcggg tgctcgggct cggcgtcctc
      481 cgacgcaggc gcatcgacgg tgggcaggtg catctcgacc gtcgtgccgc ggccgggtgc
      541 actgtgcagg cggatgtcgc cgccggactg cagcgcgaag ccgcgcacct gcgcgagccc
      601 gagcccgctg ccgcgcccga ccggcttggt cgtgaagtag gggtcggtcg cgcgcgcgag
      661 cgtctgctcg tccatgcccg taccggtatc ggtgaccgcg atcaccgcgc ggtcggggcc
      721 cgtcgagcgt gcgctgacgg tcacgcggcc gccctcgggc atggcgtcgc gggcgttgaa
      781 gatcaggttg agcagcgcga gctccagctg cgacgggtcg acgttcacgt gccgcagccc
      841 gggttcgacg tcggccacga gctccacgct ctggccgatg gccttctgca ccagctcgcg
      901 ggcgccgagc aggaagtccg cgaagttcac cgccgcgagc tgctggggcc gcgtgcggcc
      961 gaaggtcagc atctgccgca cgagctgcgc cgcgcggtgc gtcgcgcgga tcgcggcctc
     1021 cagcgtgcgc gggtgcggcg ggccgtcctt ggtgcgctcg aagacctgca gcgcggtgct
     1081 gatggtctgc aagaggttgt tgaaatcgtg ggcaaggccg ccggtcaggc gtccgatcgc
     1141 ctcgtgcttc tggccctcga gcagcttggc ctgggcgtcc tcggcctgct gcaccgcgtt
     1201 ggcgacgcgc tgctcgagcg tgcgcgtggc ctcctgcgaa tgcacgccgg cgtcgtgcag
     1261 cacggtgctg atgtcgtcga cctcgcgcac gccggtggcc agcggcgccg gcacgcgatc
     1321 ttcgcgcagc gtcgccgcgg cgtcgtgcaa ctggccgacc ggcccgctga tgcgccgcga
     1381 cgcatagacc acgagcccga ggctgatgac cagcagcgcc gccgcggcgc cgaccgcctg
     1441 cagcgtgagc acgcgcgccg tgcggctcag gccctgcgtc ggcagggcga cgacgacctg
     1501 ccagtggtag cggttgggcc ggctcacgta ggtcagcgag cgcacgccgt ccagcgtgat
     1561 cgattcgccg aagccctcgg cctcgtcgcg cgcgcggcgc ttgatctccg gccccgcgga
     1621 cacgcccagc cagcgccgcg gctcgcgcgt gcgggcgatg atcacctgct cgtcgtccat
     1681 caccgtgacc agcgcgtcct ccggccccgg ctgctgcgcg agcagcgcct ggatgctggc
     1741 cggctcgaac gcgacgccga cgttgtagcg cggcggcgcg acgcccagct cgggccggaa
     1801 cagcgcgagc accggcccgt gcgtcaccgg gccgaccggc gcgaagaaga cgtcggcggc
     1861 gtccgaggtg accatcggcg cgcccggggg acgcgcgatc cgcagccctt cctgccaggg
     1921 cacgtgcgtg ttgaggatct cggcggtcgg cgtgacgagg atgacccagc tgtcgccgcc
     1981 gcgcgctgcc gtcgcggcct cgtcatagaa gccgcgcagg tcgtcgcggc gcagcgcgag
     2041 cgacgccccc agcgtgcgcg ccatcgtcgc gcgcctgtcg agctcgcgct ccaccagcag
     2101 gttcaaggtg cgcccgtacg cgcgcaggtg ctcccggccg gtggccgtct gctgcaggaa
     2161 gatggatcgc gcgagcatgc cgaatcccac cgccgccggc agccatacgg cgaacaccaa
     2221 cagcatgagc cggatgcgga tgttcacgta gcggcgcccg atggattcga aagcctggac
     2281 gctatcagaa ttcgccgccg aggtgatcgg cgctcgccca cggttggata cagtggaggg
     2341 tatgcattcg acgacgccgc tgcggatcct gctggtcaac aactcgtccg ccgcggcctt
     2401 ccggctcgtg ccgccgcccg ctgacgccgt gctggcggac gccccgcccg ccgccacctg
     2461 gcgcgccgcc gccgagggcc aggtcgacgc g
//
LOCUS       DASVLN010000733        11861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_469656, whole genome shotgun sequence.
ACCESSION   DASVLN010000733 DASVLN010000000
VERSION     DASVLN010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11861
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469656"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VF453_17345"
     CDS             complement(<1..559)
                     /locus_tag="VF453_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6160 family protein"
                     /protein_id="HEX7689479.1"
                     /translation="MNMLKITAAAALAAASLSASAMTSIADDQLSAISGQDGVSIAAD
                     LNINIGAFTYTDTDTNGGSVSFKNITVRGMMDMTIDVLNSTAFAGAVASSIEANGGGT
                     AGSAGNTLILAGVAQALGYNPTPGAGADVVQFAFPAAVGAGAAAATHAVTPTITVGSI
                     ATGNGGASFGSFTIQNLDLQGTKVWM"
     gene            complement(1049..2077)
                     /locus_tag="VF453_17350"
     CDS             complement(1049..2077)
                     /locus_tag="VF453_17350"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEX7689480.1"
                     /translation="MVVVPLSQLSAFKAMKDGFSRRGLDLAKILEEAGLDPNPNEAVD
                     AEKLADLFSVAWSAAVERTGDPAIGLQLSPRQPLIGLGSLSSLVLSAPDLRSGLQRLA
                     RYTSLISPTTSIGMDAPATSLGMHVDHDACRITLRISAGKLPVPQQRYDFMAATFMKG
                     ILWLTGEAVTPVRVHHPYPAPADPAPWERAFGCPVVFGAAAFVIELPLSALDAPFPTS
                     DPTVGEMCERMVAQLAAEQGGCITARVRQALMQLISKGDPRRELVAATLCMSERTLQR
                     RLTEEGTSFIELVDDTRRELAQRYFVNGAYTPTEMSFALGFSDPSNFYRACKRWFGRT
                     PNTLRCAA"
     gene            complement(2515..2898)
                     /locus_tag="VF453_17355"
     CDS             complement(2515..2898)
                     /locus_tag="VF453_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEX7689481.1"
                     /translation="MADADPNDCSQDRPHDLDALRERLREFARERDWQPFHSPKNLAM
                     ALSVEAAELLEHFQWLTESESRAPAPDRLAAVGEECADVLLYLVQLADQLGIDLLDAA
                     RRKMARNALRYPVEAARGHARKADR"
     gene            complement(2932..3348)
                     /locus_tag="VF453_17360"
     CDS             complement(2932..3348)
                     /locus_tag="VF453_17360"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7689482.1"
                     /translation="MRDGEFLRLAAFREQLRQFQAFSERACDSRGLTSPQYQALLAMR
                     AHRGNDAPFTVRTLSECLLIKHNSAVGLVDRMAQLGLVERTGSPTDRRSVVLVLTAHG
                     GRVLEMLAEQHRTELHRTARAWIHCLEQLAEPPAAS"
     gene            3502..4842
                     /locus_tag="VF453_17365"
     CDS             3502..4842
                     /locus_tag="VF453_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HEX7689483.1"
                     /translation="MSQFEEFDPSDARRRDFLKLSLGAAGGLALPGLALAADYPALAN
                     YPAGVAGDSVFIGLPLDLTGPYSAEGADEQRGYELAAEQLNAGDERIRKISPLTKKGV
                     LGKKVTLGVADAETKPNTAVQAATRFIHDNKAMMIGGSVSSAVAIALQKVCDREKTVY
                     MAAISGSNETTGVDCQRYGFRLCHYAYSASKAIAPVLGASLGKNRKAMYLVPDYTYGH
                     TTHDSMVEFTGKEGWKEIGQQVHPLGTKDYSSYLINIANSGADTLVVIAYGLDAVNSI
                     KQAKQFGLLDKMKLVVPYMAPFLGEELGADIMQGVYGCMGFWWTLQDKYPVAKDFVDA
                     FQAKFKTVPHDSAYIAYLTTVLWADAVERAGTFYGPEVVKALEKGVKRQGPVGDVWFR
                     ASDHQGIINFPIVRGKKASEMKNKSDFFDIVTVANGAEVVSPEGLLGCKLGSYT"
     gene            4859..5734
                     /locus_tag="VF453_17370"
     CDS             4859..5734
                     /locus_tag="VF453_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7689484.1"
                     /translation="MPALPLVLSQLFNGLAEGLLLALISTGLTIIYGTLGIINFAHGA
                     LFVCGAYAGLLVFNATGSFLLAALAGGLVALVLGLLMERGVVQLFYGRPPEDQLLVTF
                     GIGIVFVEVVRSIFGGVGLSVSTPAWGQGVANLGFLIYPLYRLEILGISAATLAVLYV
                     VLYRTRLGLIVRAGIEDAQMVNVLGINVKRAFLLVFGLGTMAAGYAGVIDSPIESLTP
                     DMGARVLVDSFVVVVIGGVGSFPGAIFGGVIAGEIMSLTSMFDPAYSYVMLFAVMALV
                     LILRPQGLMGQEGRE"
     gene            5731..7578
                     /locus_tag="VF453_17375"
     CDS             5731..7578
                     /locus_tag="VF453_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX7689485.1"
                     /translation="MNADRRLRRDAGLWPEAALLAGLAVAPFVLPHLGFGADLMSRVL
                     IWGLFGLGFDLLFGYTGLLSFGQAAFFGVGGFVCAYMLSGSAPNMLLALLLGTLVAAV
                     AGLVIGYLTLRRTGIYFAMSTLAFAEMFYFLENSPLKAYTGGENGIAGIPTAVLPLGF
                     TTLTISSGWPMYVFIAAFYFVGYVIARRIVHSPFGAVLTAIRGNTMRAGALGHSTQAY
                     KLAVFVIAAMYGGLAGGLLGVFQSYMPPDAFMLDTSSQLVIQTVIGGAGTLLGPVLGA
                     AIWLGLYEGLQHVQGIGGYWKLILGLVFVVLITLFRHGVAGMLVDVVRRRRGAKESLQ
                     DATMEVVTPQQLADTRLELVAVPPQEAGTPVLQARGITKQYGGLVAVSEVDFALQEGE
                     IRGVIGPNGAGKSTFFKMLAGEVAPTRGSVALRGQDITGAGVTAVCHRGMSKSYQINQ
                     LFESLTVRQNMVIPALARERGRFRLDVLRRLSRMTRAATQAEAAIDLVGLARVADVPV
                     RELSYGEKRRLEIGLALATGANVLLFDEPLAGLGPEERAHVIALLKAIRGGRTMVIVE
                     HDMDAMFELAERITVLYEGRKLAEGTPAEIQQDPAVQGAYLGGLEHEPA"
     gene            7565..8269
                     /locus_tag="VF453_17380"
     CDS             7565..8269
                     /locus_tag="VF453_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689486.1"
                     /translation="MSLLEVDRLNSYYGDSHILFDVSMRVEANEVVALLGRNGAGKST
                     AMKSMMGIVDIRGGSVRLDGQDVTGRPPHQLAMQGIQLVPEERRIFGALSVHENLVLA
                     AMTAPSPRSFEDIYKVFPRLDERRRSRGKQLSGGEQQMLAIARAMIRNPRVLLLDEPF
                     EGLAPIIVRDLMRVCQQLAESGQTIVVVEQNVVAALSLANRCYILNNGHVVYDGTPGE
                     LQRERGIVERYLGVKA"
     gene            8292..8579
                     /locus_tag="VF453_17385"
     CDS             8292..8579
                     /locus_tag="VF453_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX7689487.1"
                     /translation="MAACVIGHITVLDADKWAQYKREVPATIAPWGAEVVFRGRRTAV
                     LGGAHRHDETVVLRFPDLASARAWHDSAAYRALIPLRRQAADVDLVLFDED"
     gene            complement(8656..8731)
                     /locus_tag="VF453_17390"
     tRNA            complement(8656..8731)
                     /locus_tag="VF453_17390"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8696..8698),aa:Asn,seq:gtt)
     gene            complement(8825..8900)
                     /locus_tag="VF453_17395"
     tRNA            complement(8825..8900)
                     /locus_tag="VF453_17395"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8865..8867),aa:Asn,seq:gtt)
     gene            complement(8941..11091)
                     /locus_tag="VF453_17400"
     CDS             complement(8941..11091)
                     /locus_tag="VF453_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7689488.1"
                     /translation="MSRSRSFARSPLSAAAALALAASCGTAAAQDAVQTVQVTGRGVD
                     ASDVTGFAQPLSRTPLQATTIDASALADIGATSLSDLTRLDASLTDSYNAVGYWTTFS
                     IRGFQVDNQHNFLRDGLPINAETVLALDNKSGVDILKGLSGMQAGTSAPGGLVNLVVK
                     RPDADLRDASLGWSQDGTFGAAVDLSQRFGADRAFGARLNVSAARLDPNVHDARGHRQ
                     VLALATDWRIDPGKLLEFEFEHNLQSQPSVPGVSMLGDVVPDPHSIDPRLNLNDQPWS
                     LPVVTQGNTMSLRYTQALPDDWKLKLHALQQQLVSNDHLAFDYGLYNADGSSCDPCDR
                     FSSDGRYTIWDFRSDGERRTQRVADASIAGTLSTGPLQHALTVGAQASRFTARFHDEA
                     YNLAGTGTIDGLTMVPANPAADVPQDDRTERSTELYVRDAIALGHDWTAWLGLRHAWL
                     HRGSTPTSGDMSSATDYHQAFTTPWVSVSRQLTPADMVYASWGQGVESSVTPNLPTYE
                     HPGQAEPATRSRQWEAGWKHAHGPQSWSVDAFDVRRPQYTDVADDGDAQQRLVHARDG
                     DVVSRGVEAEAQTRLAAVTLRASAMWQRVRVAGSSELDGTRPPNIPDKALKLGATWHV
                     AALPGLALLGDVGYEGGREVLPDDSARIPGWTRVDLSMRWTQPVAGARLTWRAGVDNA
                     FDRRAWREAPYQYDHAYLFPLEPRTFFASVDASF"
     regulatory      complement(11158..11268)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(11367..>11861)
                     /locus_tag="VF453_17405"
     CDS             complement(11367..>11861)
                     /locus_tag="VF453_17405"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7689489.1"
                     /translation="QVTLMHRREVFDADDATVAAFRDAQAAGALAFVAAQPTGLRTHA
                     DGRLAALEILDATGATTPLPVDTVVVLQGLSPKLGPLAEWGLALERRQVTVDTARFQT
                     SVPGLFAVGDVVHYPGKQRLVLCGFHEATLAAAGVAGHLRPGSDGPLEYTTSSPRLHR
                     LLGV"
BASE COUNT         1748 a         3917 c         4311 g         1885 t
ORIGIN      
        1 acatccagac cttggtgcct tgcaggtcca ggttctggat cgtgaacgag ccgaaggacg
       61 cgccgccgtt gccggtggcg atcgagccga cggtgatcgt cggggtcacg gcgtgcgtcg
      121 cggcagcggc gcccgcgccc acggcggcgg ggaacgcgaa ctggacgacg tcggcgccgg
      181 cgcccggggt cgggttgtag ccgagggcct gggccacgcc ggccaggatc agcgtgttgc
      241 cggcggagcc ggcggtgccg ccaccgttgg cttcgatcga cgacgcgacg gcgccggcga
      301 acgcggtgct gttcaggacg tcgatggtca tgtccatcat gccgcggacg gtgatgttct
      361 tgaacgacac ggagccgccg ttggtatcgg tgtcggtgta cgtgaacgcg ccgatgttga
      421 tgttcaggtc ggcggcgatc gacacgccgt cctggccgga gatcgcgctc agctggtcgt
      481 cggcgatgga ggtcatggcc gaagccgaca gggaggcggc ggccagggcg gcggcggcgg
      541 tgatcttgag catgttcatg tgtctctcca gatatataag gtctattgat cggaacttga
      601 aaacccctcg cgggcgttca ccgtttctgc gtcaagggtt ttgccctgga gctcgccgct
      661 cccttgtgag ggcccggact gcgctgaaac cgatacttgc gtcgcgtgcc gcaatcatca
      721 gcgccaatgc cgcgtcgcgc actgacttgc ggagccaagg acatggcttg agaaaccgtc
      781 gccggggggt atctacccta gttcttgggg tttgtccgct tcggcggcca gggcgggcgc
      841 cgagtcggcg tttcctgcga aagtccaatc ggcgcaagcc cttaggggtt cacccgcggt
      901 tcgtggggaa gacgcgattg gcccgggaag ccatcctggt ttcggcggaa atatttctcc
      961 cacgaaaaaa ggagcctgcc ggctcctttg ctggaatggg ccgaaatata tattcgaccg
     1021 gctttccgga gataactcga aaatagcttc aggcggcgca gcgaagggta ttgggcgtgc
     1081 gtccgaacca gcgtttgcag gcccggtaga aattgctcgg atcggagaat ccgagggcga
     1141 agctcatctc ggtgggcgtg taggcgccgt tgacgaagta gcgctgcgcg agctcgcggc
     1201 gggtgtcgtc gaccagttcg atgaagctgg tgccttcctc cgtgaggcga cgctgcaggg
     1261 tgcgctcgct catgcacagc gtcgcggcga ccagctcgcg gcgcggatcg cccttggaga
     1321 tcagctgcat cagtgcctgg cgcacgcgcg ccgtgatgca gccgccctgt tccgccgcca
     1381 gctgggcgac catgcgttcg cacatctcgc ccaccgtcgg gtcggaggtg gggaaggggg
     1441 cgtccagggc ggacagcggc agctcgatca cgaaggccgc cgcgccgaac accaccgggc
     1501 agccgaaggc gcgctcccac ggcgccgggt cggccggcgc cgggtaggga tggtgcacgc
     1561 gcacgggcgt gacggcctcg ccggtcagcc agaggatgcc cttcatgaag gtcgccgcca
     1621 tgaagtcgta gcgctgctgg ggcacgggca gcttgccggc ggagatgcgc agggtgatgc
     1681 ggcaggcgtc gtgatcgaca tgcatgccca gcgaggtcgc gggcgcgtcc atgccgatgg
     1741 aggtcgtcgg cgagatcagg ctggtgtagc gcgccaggcg ctgcagcccg ctgcgcaggt
     1801 cgggcgccga cagcaccagg ctggacagcg agcccaggcc gatcagcggc tggcgcggcg
     1861 acaactgcag gccgatcgcg gggtcgcccg tgcgttccac cgcggcgctc caggccacgc
     1921 tgaacaggtc ggcgagcttc tcggcgtcga ccgcctcgtt cgggttcggg tcgaggcccg
     1981 cctcttccag gatcttggcc aggtcgagtc cgcgtcgcga gaagccgtcc ttcatcgcct
     2041 tgaacgcgct gagctgagac aggggaacga caaccacggc ggtactccga agcgctggga
     2101 acgaacgatc gcgaagagct tcgcggccgg cgcattgaaa atagaacgac cgttcgattt
     2161 tcttcaggga aatgttatcg accgccctgg tgcaccgaca taggaatcaa cccgaacttt
     2221 gccccggcaa ggggcatgca gccccccaaa acaggggagt cgcggagggc cgtcgaaggg
     2281 aaaacccggt ccgtgcaccg gcttcgcgcg agcctgtttc tccgaggtgc ggcgtggacg
     2341 gcggcgcgtg gcgcgcgaag gcgggatcgc gcgatcggca aggcgggcag tgcacgatcg
     2401 tgggtcgcct gcaacatggc tcaggcggcg ggcgcggcgt tgccggggcg ctgtcgaacg
     2461 agcgtgcgga aacctcgtcg aaaccgggcg tgacttgaag cgccggtgcg gcggtcagcg
     2521 gtccgccttg cgggcatgcc cgcgcgcggc ctcgaccgga tagcgcagtg cgttgcgcgc
     2581 catcttccgg cgcgcggcgt cgagcaggtc gatcccgagc tggtcggcca gctgcaccag
     2641 gtacagcagg acgtcggcgc attcctcgcc gacggcggcc agccggtcgg gcgcgggcgc
     2701 gcggctctcc gactcggtga gccactggaa gtgctccagc agctccgcgg cctcgacgga
     2761 cagcgccatc gcgaggttct tgggcgagtg gaacggctgc cagtcccgct cccgggcgaa
     2821 ctcgcgcagg cgttcacgca gggcgtcgag atcgtgcggg cggtcttgcg agcaatcgtt
     2881 cgggtcggca tcggccatgg gcgcagcata gccggccgcc gcggcgcggc cctaggacgc
     2941 cgccggcggt tccgcgagct gctccaggca gtggatccac gcccgcgccg tgcgatggag
     3001 ttcggtgcgg tgctgttcgg ccagcatctc gaggactcgc ccgccgtggg cggtgagcac
     3061 caggacgacg ctgcggcggt cggtcggcga tccggtgcgc tcgaccagcc cgagctgcgc
     3121 catccggtcc acgagcccga cggcgctgtt gtgcttgatc agcaggcatt ccgacagcgt
     3181 gcgcacggtg aagggcgcgt cgttgccgcg atgcgcccgc atggccagca gcgcctggta
     3241 ctggggcgag gtcaggccgc gggagtcgca ggcgcgctcg ctgaaggcct ggaactgccg
     3301 caactgctct cggaacgcgg cgagccgcag gaattcccca tccctcatgg accagccttt
     3361 ctgacggctt acaacggcaa gcgggccgtg ggcgtacttt caccgatgac gatatatcgt
     3421 gacaggacgt actctgaatg taagagatgt cgcgcgacgc ctgtcgcgaa cctgtcccag
     3481 ccatccgcca ggagacgtgc cgtgagccag ttcgaggagt tcgaccccag cgacgcgcgt
     3541 cggcgcgatt tcctgaaact gagcctcggc gcggccggcg ggctcgcctt gccggggctg
     3601 gccctggcgg cggactatcc cgccctggcc aactacccgg ccggcgtcgc gggcgattcc
     3661 gtgttcatcg gccttccgct ggacctcacg ggcccctatt cggccgaggg cgccgacgag
     3721 cagcgcggct acgagctcgc cgccgaacaa ctcaatgcag gcgacgagcg catccgcaag
     3781 atctcgccgc tcaccaagaa gggcgtgctg ggcaagaagg tgacgctggg cgtggccgac
     3841 gccgagacca agccgaacac cgcggtgcag gcggcgacgc gcttcatcca cgacaacaag
     3901 gcgatgatga tcggcggctc ggtgagcagc gcggtcgcga tcgcgctgca gaaggtctgc
     3961 gaccgcgaga agaccgtcta catggcggcc atcagcggct ccaacgagac gaccggcgtc
     4021 gactgccagc gctacggctt ccgcctgtgc cactatgcct actcggcttc caaggcgatc
     4081 gcgccggtgc tcggggcgtc gctgggcaag aaccgcaagg cgatgtacct cgtgccggac
     4141 tacacgtacg gccacacgac ccacgactcg atggtcgagt tcaccggcaa ggaaggctgg
     4201 aaggaaatcg ggcagcaggt ccacccgctg ggcaccaagg actacagctc ctacctgatc
     4261 aacatcgcca acagcggggc cgacacgctg gtggtgatcg cctacggcct cgacgcggtg
     4321 aactcgatca agcaggccaa gcagttcggc ctcctggaca agatgaagct ggtggtgccg
     4381 tacatggcgc ccttcctcgg cgaggagctg ggcgccgaca tcatgcaggg cgtctacggc
     4441 tgcatgggct tctggtggac gctgcaggac aagtaccccg tcgccaagga cttcgtcgac
     4501 gcgttccagg ccaagttcaa gacggtgccc cacgactccg cctacatcgc ctacctcacc
     4561 accgtgctgt gggcggacgc ggtcgagcgt gccgggacgt tctacgggcc ggaggtcgtg
     4621 aaggcgctcg agaagggcgt caagcgccag gggccggtgg gcgacgtgtg gttccgcgcc
     4681 agcgaccacc agggcatcat caacttcccc atcgtgcgcg ggaagaaggc cagcgaaatg
     4741 aagaacaaga gcgacttctt cgacatcgtc acggtggcca acggcgcgga ggtggtgtcg
     4801 cccgaagggc tgctgggctg caagctgggc tcgtacacct gacgcaaggg acgcgcccgt
     4861 gcccgcattg ccgctggtcc tgtcccaact gttcaacggc ctcgccgaag gattgctcct
     4921 cgcgctcatc agcacgggcc tgacgatcat ctacggcacg ctgggcatca tcaacttcgc
     4981 ccacggcgcg ctgttcgtct gcggcgccta cgcagggctg ctggtgttca acgccaccgg
     5041 ttcgttcctg ctcgcggcgc tggccggcgg gctggtcgcg ctggtgctgg gcctgctgat
     5101 ggaacgcggc gtcgtgcagc tgttctacgg gcgccctccg gaggaccagc tgctggtcac
     5161 cttcggcatc ggcatcgtgt tcgtcgaggt ggtgcgctcg atcttcggcg gcgtcgggct
     5221 gtcggtgtcg acgcctgcgt ggggccaggg cgtggccaac ctcggcttcc tcatctatcc
     5281 gctgtaccgg ctcgagatcc tcggcatctc cgcggcgacg ctggctgtcc tctacgtggt
     5341 gctgtatcgc acccggctcg gcctgatcgt gcgggcgggc atcgaggacg cgcagatggt
     5401 caacgtgctg ggcatcaacg tcaagcgcgc gttcctgctg gtgttcggcc tcggcacgat
     5461 ggccgcgggc tatgccggcg tgatcgattc gccgatcgag tcgctcacgc ccgacatggg
     5521 cgcgcgcgtg ctggtcgact ccttcgtcgt cgtcgtgatc ggcggcgtcg gctcgttccc
     5581 cggcgcgatc ttcggcggcg tgatcgccgg cgagatcatg agcctgacct ccatgttcga
     5641 ccccgcctat tcctacgtca tgctgttcgc cgtgatggcg ctcgtgctga tcctgcggcc
     5701 gcagggcctg atgggccagg agggacgcga gtgaacgcgg atcgccggct gcggcgcgac
     5761 gcaggactct ggccggaggc cgcgctgctg gccggcctgg ccgtcgcccc cttcgtgctg
     5821 ccccacctcg gcttcggcgc cgacctgatg agccgcgtcc tgatctgggg cctgttcggc
     5881 ctcggcttcg acctgctgtt cggctacacg gggctgctgt ccttcggcca ggcggcattc
     5941 ttcggcgtgg gcggcttcgt gtgcgcctac atgctgagcg gcagcgcgcc caacatgctg
     6001 ctcgcgctgc tgctcgggac gctggtcgcg gcggtcgcgg gcctcgtgat cggctacctg
     6061 acgctgcgcc gcacgggcat ctacttcgcg atgagcacgc tcgccttcgc ggagatgttc
     6121 tattttctgg agaactcgcc gctgaaggcc tacaccggcg gcgagaacgg catcgccggg
     6181 atcccaacgg ccgtgctgcc gctgggcttc acgacgctga cgatctccag cggctggccc
     6241 atgtacgtct tcatcgcggc gttctatttc gtcggctacg tgatcgcgcg tcgcatcgtc
     6301 cactcgccgt tcggggccgt gctcacggcg atccgcggca acacgatgcg agcgggcgcg
     6361 ctcgggcatt ccacgcaggc ctacaagctg gccgtgttcg tcatcgcggc gatgtacggc
     6421 ggcctcgcgg gcgggctgct gggcgtgttc cagagctaca tgccgccgga cgccttcatg
     6481 ctcgacacct cgtcgcagct ggtgatccag accgtcatcg ggggcgcggg cacgctgctc
     6541 ggcccggtgc tcggtgccgc gatctggctg ggcctctacg aaggcctgca gcacgtgcag
     6601 ggcatcggcg gctactggaa gctgatcctg ggcctcgtgt tcgtggtgct catcacgctg
     6661 ttccgccatg gggtggccgg gatgctcgtc gacgtcgtac gcaggcgccg cggcgccaag
     6721 gagagcctgc aggacgcgac gatggaggtc gtcacgccgc agcagctcgc ggacacgcgg
     6781 ctcgagctcg tcgccgtgcc gccccaggaa gccgggacgc cggtcctgca ggcgcgcggc
     6841 atcacgaagc agtacggcgg tctggtcgcg gtctctgagg tcgacttcgc gctgcaggag
     6901 ggcgagatcc gcggcgtgat cggtcccaac ggcgccggca agtcgacgtt cttcaagatg
     6961 ctggccggcg aggtcgcgcc gacgaggggc agcgtggcgc tgcgcggcca ggacatcacg
     7021 ggcgcgggcg tcaccgcggt gtgccatcgc ggcatgagca agagctacca gatcaaccag
     7081 ctcttcgagt cgctgacggt gcggcagaac atggtgatcc cggcgctggc gcgcgagcgc
     7141 ggccgcttcc ggctcgacgt gctgcgccgg ctctcgcgca tgacgcgcgc ggcgacgcag
     7201 gcggaggcgg cgatcgatct ggtgggtctc gcgcgcgtgg ccgacgtgcc ggtgcgcgag
     7261 ctgtcctacg gcgagaagcg gcgcctggag atcggcctcg cgctggccac cggcgccaac
     7321 gtgctgctgt tcgacgagcc cctggccggc ctggggcccg aggagcgcgc acacgtgatc
     7381 gcgctgctga aggcgatccg tggcggccgc acgatggtga tcgtggaaca cgacatggac
     7441 gcgatgttcg aactggccga gcgcatcacg gtgctctacg agggccgcaa gctggccgaa
     7501 ggcacgcccg ccgagatcca gcaggatccg gccgtgcagg gcgcgtacct ggggggcctc
     7561 gagcatgagc ctgcttgaag tcgaccgcct caacagctac tacggcgact cgcacatcct
     7621 cttcgatgtc tcgatgcggg tggaggccaa cgaggtcgtg gcgctgctcg ggcgcaacgg
     7681 cgcgggcaag agcacggcga tgaagtcgat gatgggcatc gtcgacatcc gcggcggcag
     7741 cgtccgcctg gacggccagg acgtcaccgg caggccgccg caccagctgg cgatgcaggg
     7801 catccagctc gtgcccgagg aacggcgcat cttcggcgcg ctgagcgtgc acgagaacct
     7861 cgtgctcgcg gcgatgacgg cgccgtcccc gcgcagcttc gaggacatct acaaggtctt
     7921 cccgcggctc gacgagcgcc gccgctcgcg cggcaagcag ctctcgggcg gcgagcagca
     7981 gatgctcgcg atcgcccgcg cgatgatccg caacccgcgc gtgctgctgc tcgacgagcc
     8041 gttcgagggc ctcgcgccga tcatcgtgcg cgatctcatg cgggtgtgcc agcagcttgc
     8101 ggagtccggg cagaccatcg tcgtggtcga gcagaacgtg gtggccgcgt tgtcgctggc
     8161 caatcgctgc tacatcctca acaacggcca cgtggtgtac gacggcacgc cgggcgagct
     8221 gcagcgcgag cgcggcatcg tcgaacgcta cctcggcgtg aaggcctgag gaaaccgggg
     8281 gagcccgcgt cgtggcggcc tgcgtgatcg gacacatcac cgtgctcgac gcggacaagt
     8341 gggcgcagta caagcgcgag gtgccggcga cgatcgcgcc ctggggcgcc gaggtggtgt
     8401 tccgcggacg ccggaccgcc gtgctgggcg gcgcgcatcg ccatgacgaa acggtggtgc
     8461 tgcgctttcc cgacctcgcc tctgcgcgcg cctggcacga ctcggcggcc taccgggccc
     8521 tgatcccgct gcgccggcag gcggccgacg tcgacctggt cctcttcgac gaggattgag
     8581 ccgcggacgc tggtctcgcg ggttccggaa aggacgacgg cccgaggctt ctcgcctgcg
     8641 ggccgttcgc ttggatggct ccccgacctg ggctcgaacc agggacctac ggattaacag
     8701 tccggcgctc taccgactga gctatcgggg aatcgaggcc gctcctcgcg gatcggctcg
     8761 cgaaatctcg gtcatgaaaa aaggcctttc gttgcgacaa ggccttccat cgcttcgcgt
     8821 tcggtggctc cccgacctgg gctcgaacca gggacctacg gattaacagt ccggcgctct
     8881 accgactgag ctatcgggga atcgcgaagc cttgcagtct agcgtggttt tctggtttcg
     8941 tcaaaagctc gcgtcgacgg acgcaaaaaa agtgcgcggc tcgagcggga acaggtacgc
     9001 gtggtcgtac tggtaggggg cctcgcgcca ggcgcggcgg tcgaaggcgt tgtcgacgcc
     9061 cgcccgccag gtcaggcggg cgccggccac gggctgggtc cagcgcatgg acagatccac
     9121 gcgcgtccag ccggggatgc gggcgctgtc gtccggcagc acctcgcggc cgccttcgta
     9181 acccacgtcg cccagcagcg cgaggcccgg cagcgcggcg acgtgccagg tcgcgccgag
     9241 cttgagcgcc ttgtccggga tgttgggcgg gcgcgtgccg tcgagctcgg acgacccggc
     9301 cacccgcacg cgctgccaca tggcgctcgc ccgcagcgtc actgcggcaa ggcgggtctg
     9361 cgcctcggcc tcgacgccgc gcgagacgac atcgccgtcg cgggcgtgca cgaggcgttg
     9421 ctgggcgtcg ccgtcgtcgg ccacgtcggt gtactgcggc cggcggacgt cgaatgcgtc
     9481 cacgctccag ctctgcgggc cgtgcgcgtg cttccagccg gcctcccact ggcggctgcg
     9541 ggtggcgggt tcggcctggc cgggatgctc gtaggtgggc aggttagggg tgacgctgct
     9601 ctcgacgccc tggccccagc tcgcgtagac catgtcggcc ggcgtcagct ggcgcgagac
     9661 cgacacccac ggcgtggtga acgcctggtg gtagtccgtc gcggaggaca tgtcgcccga
     9721 ggtcggggtc gagccccggt gcagccacgc gtggcgcagg cccagccacg cggtccagtc
     9781 gtggccgagc gcgatcgcgt cgcgcacgta gagctccgtg ctgcgctcgg tgcggtcgtc
     9841 ctgcggcacg tcggccgcgg gattggccgg caccatcgtc agtccgtcga tcgtgccggt
     9901 acccgcgagg ttgtacgcct cgtcgtggaa gcgcgccgtg aagcgcgagg cctgggcccc
     9961 gacggtcagc gcatgctgca acggcccggt cgagagcgtg cccgcgatgg aggcgtccgc
    10021 cacgcgctgg gtgcgtctct cgccgtcgct gcggaagtcc cagatcgtgt accgcccgtc
    10081 gctcgagaac cggtcgcacg ggtcgcagct cgagccgtcg gcgttgtaca ggccgtagtc
    10141 gaacgccagg tggtcgttgc tcacgagctg ctgctgcagc gcgtgcagct tgagcttcca
    10201 gtcgtccggc agcgcctgcg tgtagcgcag cgacatcgtg ttgccctgcg tcacgaccgg
    10261 cagcgaccac ggctggtcgt tgaggttcag gcgcgggtcg atcgaatgcg gatcgggcac
    10321 cacgtcgccg agcatgctca cgcccgggac gctcggctgc gactgcaggt tgtgctcgaa
    10381 ctcgaactcg agcagcttgc ccgggtcgat gcgccagtcg gtcgcgagcg cgaggacctg
    10441 gcggtgcccg cgcgcgtcgt gcacgttcgg gtcgagccgc gcggccgaga cgttcagccg
    10501 cgccccgaat gcgcggtcgg cgccgaagcg ctggctcagg tcgaccgccg cgccgaaggt
    10561 gccgtcctgc gaccagccga gcgaggcgtc gcgcaggtcg gcgtcgggcc gcttgaccac
    10621 gaggttcacg aggccgccgg gcgcgctcgt gccggcctgc atgccgctca ggcccttcag
    10681 gatgtccacg cccgacttgt tgtccagggc cagcaccgtc tccgcgttga tcggcaggcc
    10741 gtcgcgcagg aagttgtgct ggttgtcgac ctggaagccg cggatcgaga acgtcgtcca
    10801 gtagccgacc gcgttgtacg agtcggtcag gctcgcgtcc aggcgcgtga ggtcggacag
    10861 cgaggtcgcg ccgatgtcgg cgagcgcgga ggcgtcgatc gtcgtcgcct gcaggggcgt
    10921 gcgcgacagc ggttgcgcga agccggtgac gtcggaggcg tcgacgccgc gaccggtgac
    10981 ctgcacggtc tggacggcgt cctgcgcggc ggcggtgccg caggaggcgg cgagggcgag
    11041 cgccgcggcc gccgacagcg gcgagcgggc gaacgaacgg gaacgggaca agcgggccat
    11101 cgagctcttt cgaagcgatg gcagggaacg gtgtcgggag tcgagagcgc gaacggcttc
    11161 gtggtgcgac cgcttccctg cgcgaggatt acctcaatca ggttcaaagg gactttctca
    11221 gtcgggcgct gcacgcgagc gtgcggccca acacccctag cgaagatgcg cgtcgcccgc
    11281 gggcggacga cggcgcgggc gtgattatgg cagcgtgggc ggcaggctcc gcgttttccc
    11341 gcggcccgcg tggcgccgac gcgtcgtcag acccccagca ggcggtgcag gcgcgggctg
    11401 ctggtcgtgt attcgagcgg gccgtcgctg cccgggcgca gatggccggc gacgcccgcg
    11461 gcggcgagcg tcgcctcgtg gaagccgcac agcacgagcc gctgcttgcc gggatagtgc
    11521 acgacgtcgc cgaccgcgaa cagccccggc acgctggtct ggaagcgcgc cgtgtcgacc
    11581 gtgacctggc gccgctcgag cgcgaggccc cactcggcca gcggcccgag cttggggctc
    11641 aggccctgca gcacgacgac cgtgtcgacg ggcagcggcg tcgtcgcgcc ggtcgcgtcg
    11701 aggatctcga gcgcggcgag ccggccgtcg gcgtgcgtcc gcagtccggt tggctgtgcg
    11761 gcgacgaagg ccagcgcgcc ggccgcctgc gcgtcgcgga acgcggcgac ggtcgcgtcg
    11821 tcggcatcga agacctcgcg ccggtgcatg agcgtcacct g
//
LOCUS       DASVLN010000734         6179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_413437, whole genome shotgun sequence.
ACCESSION   DASVLN010000734 DASVLN010000000
VERSION     DASVLN010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413437"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VF453_17410"
     CDS             complement(<1..686)
                     /locus_tag="VF453_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7689490.1"
                     /translation="MPQANAPKTWFITGTSTGFGLSFAKHALERGDNVVATARNPAAL
                     EPLAASAPDRVLAVALDVTRPGAAQAAVDAAVARFGRIDVVVNNAGYGSVGAVEETPE
                     PELRAQLETNFFGAVAVIRAALPALREQRSGAIVNVSSMGGNLSFAGFGAYSASKFAL
                     EGLSEALAQEVAPFGIKVLIVEPGAFRTAFAGGSLKRMPEIAAYRETAGASRAFADGM
                     HGTQAGDPDKA"
     gene            814..1761
                     /locus_tag="VF453_17415"
     CDS             814..1761
                     /locus_tag="VF453_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7689491.1"
                     /translation="MDRDLLGFLPVVASVARYRSFAAAAAALDMSASAVSHAVKAAED
                     RLGEPLFARTTRSVALTEAGAAFVATVSAALEDIGAAVEGMNAARGELSGVLRINAPR
                     VATSMALAPVLAVLARRHPRLVVEVRTEDALVDIVAAGFDAGVRLGESIAQDMVTVRL
                     TPPFEAVLVAAPSYLQARGEPAAIADLAAHDCIGFRLLASGSLYEWELAGDDGRTIAV
                     RTTGSAVITDATFARDLALAGVGIAYIFEPLVRADLAAGRLRQLLPHTALREEGLFLY
                     FPRRAAMAPKLRAFIDAARELLEAAAPACAQAARPAGMR"
     gene            complement(1797..3236)
                     /locus_tag="VF453_17420"
     CDS             complement(1797..3236)
                     /locus_tag="VF453_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7689492.1"
                     /translation="MPSPERFDPSLPRLRRFGDLMMLAGCLMGSGLSVVLGWQYGHLA
                     LACAGTLLLSGAAALCVVAARGSQANSALLPVLLMAQVAFNIHLGGGRAEYHFAVFTS
                     LTFLLAYRHWAPLVVGAVAITAHHLAFDRLQALGWPVYCQATPDFLGTLHHISYIAAS
                     TGLGLFLSTSMRQDALLSEELRCITDGVAHQHGKIDFSQLEVPVRSRPGVRLLGIFKD
                     IRRSVQVARQSVNAVGAASAEISGGNRDLSQRTEKTAADLQRTASAIEQLTQAVGMAA
                     ESTSQAAGIATTAAERAGEGERALALLAETMGRLVDSSRQVTDITGLIDSIAFQTNIL
                     ALNAAVEAARAGEHGRGFAVVASEVRHLAKRSAQAAGDIKTLIARSGQQVEAGVAVAR
                     QTQAALTAIIAEVQRVSGMLREVATSASEQSRGIGEVNQAVNELDQATQQNAALVEES
                     AAASDALEAQAGQLADAMAVFELEPSAGA"
     gene            complement(3460..4110)
                     /locus_tag="VF453_17425"
     CDS             complement(3460..4110)
                     /locus_tag="VF453_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX7689493.1"
                     /translation="MKFTAFERTLQGTGASRRLRISGKVPGIVYGAGEPQMIELDHNA
                     LYFALKKEAFHSSVLEMELNGKTSQVLLRDFQMHAWKQIVMHIDFQRVDATTRLRKKV
                     PLHFSNEENSPAVKTDKCLVNHVANEVEIEFLATALPEFISVDLGDLVKGKSLHVSDL
                     KFPAGIKAVRHGTQNPVVVSVVEPKAEEEAPAADAAAAPAAPAKGKGGKAAAPAKK"
     gene            complement(4211..5173)
                     /locus_tag="VF453_17430"
     CDS             complement(4211..5173)
                     /locus_tag="VF453_17430"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX7689494.1"
                     /translation="MLFNTVLFTGNANPVLAQEITTQLGTELGKAKVGRFSDGECDVE
                     IHQNVRARDVFVVQSTGAPTNENLMELLIMVDALKRASARRITAVIPYFGYARQDRRS
                     RSTRVPISAKVVANLLQTVGVERLLTMDLHADQIQGFFDIPVDNIYASPVLLTDVKSK
                     NYRDLVVVSPDVGGVVRARALAKQLGCDLAIIDKRRPKANVSEVMHVIGDIDGRNCVI
                     MDDMIDTAGTLVKAAEVLKDRGAKSVFAYCTHPVFSGPAVERIKNSALDEVVITNTLP
                     LSDAGKACKKIRQLSVAFLFAETIRRISDGESVTSLFAEQNNNF"
     gene            complement(5281..5357)
                     /locus_tag="VF453_17435"
     tRNA            complement(5281..5357)
                     /locus_tag="VF453_17435"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5321..5323),aa:Gln,seq:ttg)
     gene            complement(5398..>6179)
                     /gene="ispE"
                     /locus_tag="VF453_17440"
     CDS             complement(5398..>6179)
                     /gene="ispE"
                     /locus_tag="VF453_17440"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX7689495.1"
                     /translation="VLIDWADTIHLERRADGRLQRHDLGPALPPDDLALRAARALQQA
                     SGCTLGADISVLKAVPWGAGMGGGSSDAATVLLGLNRLWGLGWPRARLLELAAPLGAD
                     VPFFVGGDNAFVEGIGERLTPIDWTPRTYAVVKPAEALATALIFSHPGLARDTEPATL
                     AGSVAQLNAQQGSVNGNGFGRNDLQPVAQALCPPIAQVAKWLDARFGNSRMTGSGSAV
                     FATVGMPGDPVPEGGLDDLPDGWIGRMCRSLPRHPLRDWAS"
BASE COUNT          863 a         2087 c         2287 g          942 t
ORIGIN      
        1 gccttgtcgg gatcgccggc ctgcgtgccg tgcatgccgt cggcgaaggc gcggcttgcg
       61 ccggcggtct cgcggtaggc cgcgatctcg ggcatgcgct tgagcgaccc gccggcgaac
      121 gcggtgcgga aggcgcccgg ctcgacgatc agcactttga tcccgaaagg cgcgacctcc
      181 tgggccagcg cctcggacag tccctcgagc gcgaacttcg atgccgagta cgcgccgaag
      241 cccgcgaacg agaggttgcc gcccatgctg gagacgttga cgatcgcgcc cgagcgctgc
      301 tcgcgcaggg cgggcagcgc ggcgcggatg acggcgacgg cgccgaagaa gttggtctcc
      361 agctgcgcgc gcagctcggg ctcgggggtt tcctcgaccg cgccgacgct gccgtagccg
      421 gcgttgttga cgacgacgtc gatgcggcca aagcgggcca ccgcggcgtc cacggcggcc
      481 tgcgcggcgc ccggccgggt cacgtccagc gcgacggcca gcacgcggtc gggcgcggac
      541 gcggccagcg gctcgagcgc cgccggattg cgggcggtgg cgacgacgtt gtcaccgcgc
      601 tccagcgcgt gcttcgcgaa ggacaggccg aagccggtcg aggtcccggt gatgaaccag
      661 gtcttggggg cgttggcttg gggcatggtg atctccttgg gtgggttgtc gaggatggcg
      721 cgaggtgttc ggcgcgctgg aaaccactgt atttgccgca atccgtgaga taaatgggcg
      781 tgaatcgcat gggtcatgcg atggggtgca tgaatggatc gcgatctcct gggtttcctt
      841 ccggtcgtgg cgagcgtcgc caggtaccgc agcttcgcgg ccgccgcggc ggcgctcgac
      901 atgagcgcct cggccgtcag ccatgccgtc aaggcggcgg aggaccggct cggcgagccg
      961 ctgttcgcgc gcacgacgcg cagcgtggcc ctgaccgagg ccggcgcggc gttcgtcgcg
     1021 acggtgtcgg cggcgctgga ggacatcggc gcggccgtcg agggcatgaa cgcggcgcgc
     1081 ggcgaactct cgggcgtgct gcgcatcaac gcgccgcgcg tggcgacgtc gatggcgctc
     1141 gcgccggtgc tggcggtgct cgcgcggcgc catccgcgcc tggtcgtgga ggtgcgcacc
     1201 gaggacgcgc tggtcgacat cgtcgccgcc ggcttcgacg ccggcgtgcg cctgggcgag
     1261 tccatcgcgc aggacatggt gaccgtgcgc ctgacgccgc cgttcgaggc ggtgctcgtc
     1321 gccgcgccgt cgtacctgca ggcgcgcggc gagccggccg ccatcgccga cctggccgcg
     1381 cacgactgca tcggcttccg cctgctggcg tccggcagcc tctacgagtg ggaactcgcc
     1441 ggcgacgacg gccgcacgat cgccgtgcgc accaccggca gcgccgtgat cacggacgcc
     1501 accttcgcgc gcgacctggc gctcgcgggc gtgggcatcg cctacatctt cgagccgctg
     1561 gtgcgcgcgg acctcgccgc cggccgcctg cgccagctgt tgccgcacac cgcgctgcgc
     1621 gaggagggcc tgttcctcta cttccctcgg cgggcggcga tggcgccgaa gctgcgggcg
     1681 ttcatcgacg cggcgcggga gcttctcgag gcggcggcgc ccgcctgcgc ccaggcggcc
     1741 cgtccggcgg gaatgcgctg acccgccggg cggcggcgcc tcggcttccg gggcggtcag
     1801 gcacccgcgg aaggctccag ttcgaagacc gccatcgcgt cggcgagttg accggcctgc
     1861 gcttcgagcg cgtcggaggc cgcggccgat tcctcgacga gcgcggcgtt ctgctgggtc
     1921 gcctggtcca gctcgttgac ggcctggttg acctcgccga tcccgcggct ctgctcgctc
     1981 gcggaggtgg ccacctcgcg cagcatgccg ctgacgcgct gcacctcggc gatgatggcc
     2041 gtcagcgcgg cctgcgtctg gcgggctacc gcgacgccgg cctcgacctg ctgcccggag
     2101 cgggcgatca gcgtcttgat gtcgccggcc gcttgcgcac tgcgcttggc gagatgccgc
     2161 acctcggagg ccacgacggc gaacccgcgc ccgtgctcgc cggcgcgcgc ggcttccacc
     2221 gcggcgttga gcgccaggat gttggtctgg aacgcgatgc tgtcgatcag gccggtgatg
     2281 tccgtcacct gccgcgagct gtcgaccagc ctgcccatgg tctccgcgag cagcgccagc
     2341 gcgcgctcgc cttcgccggc gcgctcggct gccgtggtcg cgattcccgc cgcctgggac
     2401 gtcgactcgg ccgccatgcc caccgcctgg gtgagctgct cgatcgcgct ggcggtgcgc
     2461 tgcaggtcgg cggcggtctt ctcggtgcgc tgcgacaggt cgcggttgcc gccggagatc
     2521 tccgcggacg ccgcgcccac cgcattcacc gactgccgcg cgacctgtac cgaccggcgg
     2581 atgtccttga agatcccgag cagccggacg ccggggcggc tgcgcacggg aacctccagc
     2641 tgggagaagt cgatctttcc gtgctggtgg gcgacgccgt ccgtgatgca gcgcagttcc
     2701 tccgagagca gggcgtcctg gcgcatcgac gtcgacagga acaggccgag gccggtggag
     2761 gcggcgatgt agctgatgtg atgcagcgtg ccgaggaagt ccggcgtcgc ctggcagtag
     2821 acgggccagc cgagggcctg gagccggtcg aaggcgaggt ggtgcgcggt gatggccacg
     2881 gcgccgacga cgagcggcgc ccagtggcgg taggcgagca ggaacgtgag cgacgtgaag
     2941 acggcgaagt gatactccgc acggccgccg ccgaggtgga tgttgaaggc cacctgcgcc
     3001 atcagcagca cgggcagcag ggcgctgttg gcctggctgc cgcgtgcggc gacgacgcac
     3061 agcgcggcgg cgccggacag caggagggtg cccgcgcagg cgagcgccag gtggccgtac
     3121 tgccagccca gcaccacgga cagcccgctg cccatcaggc agccggccag catcatcagg
     3181 tctccgaagc gtcgcaggcg gggcaggctc ggatcaaagc gttcggggga gggcatgggt
     3241 tcggtctcaa cggttcgaca ggggacacga actcgtttcg gcctgcgcaa gccgcgcttg
     3301 atggcgccgc cgccgcaggt gcatgccggc ttgcgctgga tcaaatccgg cggcggcccc
     3361 ggcgcggcgc aggtgcggtg cccatccccg gaaaacgaaa aagccccgcc gaagcggggc
     3421 cttgttcgga gcggacacgc ggcgcgaggc cgcgcggcat cacttcttgg cgggagcagc
     3481 ggccttgccg cccttgccct tggccggggc ggccggcgcg gcagcggcgt cggcggccgg
     3541 ggcttcctct tcggccttcg gctcgacgac cgagacgacc accgggttct gcgtgccgtg
     3601 gcggacggcc ttgatgccgg ccgggaactt caggtcggac acgtgcagcg acttgccctt
     3661 gaccaggtcg cccaggtcga ccgagatgaa ctcgggcagc gcggtggcca ggaactcgat
     3721 ctccacttcg ttggcgacgt ggttgaccag gcacttgtcg gtcttgacgg ccggcgagtt
     3781 ctcttcgttg gagaagtgca gcggcacctt cttgcgcagg cgcgtcgtgg cgtccacgcg
     3841 ctggaagtcg atgtgcatga cgatctgctt ccaggcgtgc atctggaagt cgcgcagcag
     3901 gacctgcgag gtcttgccgt tcagctccat ctccagcacg gacgagtgga acgcttcctt
     3961 cttcagcgcg aagtacaggg cgttgtgatc gagctcgatc atctggggct cgccggcacc
     4021 gtagacgatg cccggcacct tgccggaaat gcgcaggcgg cggctcgctc cggtcccctg
     4081 cagggtgcgc tcgaaagcgg tgaacttcat ggtcttctcc aggtgtgggc gcccgcgacc
     4141 aggccgcccg tagaaggatc ccccaccgct gctgtgctcg aggcacgcgg ccggagatcc
     4201 gggatgcggg tcagaagttg ttgttctgct ccgcgaaaag cgaggtgacc gattcgccgt
     4261 ccgagatccg gcggatggtc tcggcgaaca ggaaggcgac cgacagctgg cggatcttct
     4321 tgcacgcctt gccggcgtcc gacagcggca gcgtgttggt gatcacgacc tcgtcgagcg
     4381 ccgagttctt gatgcgctcc acggccgggc cggagaacac ggggtgcgtg cagtacgcga
     4441 agacgctctt ggcgccgcgg tccttgagca cctcggcggc cttcacgagc gtgccggcgg
     4501 tgtcgatcat gtcgtccatg atcacgcagt tgcggccgtc gatgtcgccg atgacgtgca
     4561 tgacttccga cacgttggcc ttcgggcggc gcttgtcgat gatggccagg tcgcagccga
     4621 gctgcttggc cagcgcgcgc gcgcgcacga cgccgccgac gtccggcgag accaccacga
     4681 ggtcgcggta gttcttcgac ttcacgtcgg tcagcagcac cggcgaggcg tagatgttgt
     4741 cgaccgggat gtcgaagaag ccctggatct ggtcggcgtg caggtccatc gtgagcagtc
     4801 gctcgacgcc gacggtctgc agcaggttgg cgaccacctt ggcgctgatg ggcacgcgcg
     4861 tggagcgcga gcggcgatcc tggcgcgcgt agccgaagta ggggatcacg gcggtgatgc
     4921 ggcgcgcgct ggcgcgcttc aacgcatcga ccatgatcag gagctccatc aggttctcgt
     4981 ttgtcggcgc cccggtggac tggacgacga agacgtcgcg cgcgcggacg ttctggtgga
     5041 tctcgacgtc gcactcgccg tccgagaagc ggccgacctt cgccttgccg agctcggtgc
     5101 cgagctgggt cgtgatctcc tgtgccagca cagggttcgc gttgccggtg aacaggacgg
     5161 tgttgaacag cacgtcggga actccgtggg aggcagggcg catggaaagc gtgcgaccgg
     5221 ccacgagggg ccggtcggat gggccgggac gctcaggtca ggcgtccggc ggaatggatt
     5281 tggcagggga ggaaggactc gaaccctcgc atgtcggaat caaaatccga tgccttgacc
     5341 aacttggcga ctcccctaca caggacccgg ccagacgacc gtgccctcga aaccttctca
     5401 gctcgcccaa tcccgcaacg ggtgcctggg caggctgcgg cacatccttc cgatccaacc
     5461 gtcgggcagg tcgtcgagac cgccttcggg caccggatcg cccggcatgc cgaccgttgc
     5521 gaacaccgcg ctgcccgatc cggtcatccg gctgttgccg aaccgcgcat ccagccactt
     5581 cgcgacctgc gcgatgggtg gacagagcgc ctgggccacc ggctggaggt cgttgcgacc
     5641 gaagccgttg ccgttcaccg aaccttgctg cgcattcaac tgtgcaacag agcccgctag
     5701 tgtagcaggc tcggtgtcgc gtgccaagcc cggatgcgaa aagatgaggg ccgtggccaa
     5761 cgcttcggcc ggcttgacca cggcgtaggt ccgcggcgtc cagtcgatcg gcgtcagccg
     5821 ctcgccgatg ccctcgacga aggcgttgtc gccgccgacg aagaacggca cgtcggcgcc
     5881 cagcggcgcg gccagctcga gcagccgcgc ccgcggccag ccgagtcccc agagccggtt
     5941 caggccgagc aggacggtcg cggcatccga gctgccgccg cccatgccgg cgccccaggg
     6001 caccgccttc agcaccgaga tgtcggcgcc cagcgtgcag ccgctggcct gctgcagggc
     6061 gcgcgcggcg cgcagggcga ggtcgtcggg cggcagcgcg gggccgaggt cgtggcgctg
     6121 cagccggccg tcggcgcggc gctcgaggtg gatcgtgtcg gcccagtcga tcagcacga
//
LOCUS       DASVLN010000735         3905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_522775, whole genome shotgun sequence.
ACCESSION   DASVLN010000735 DASVLN010000000
VERSION     DASVLN010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522775"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VF453_17445"
     CDS             complement(<1..765)
                     /locus_tag="VF453_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7689496.1"
                     /translation="MLYPELFKQLEAVRWDMDKDIPWESFDPSLLTDEQATTIKMNAI
                     TEWAALPATEMFLRDNRDDSDFSAFMSVWFFEEQKHSLVLMEYLRRFRPELVPTEEEL
                     HAVRFEFDPAPALETLMLHFCGEIRLNHWYRRAAQWHTEPVIKHIYETLARDEARHGG
                     AYLRYMKRALMRFGDEAKAAFSKVGVLMASARRTAQALHPTNLHVNVKLFPRDTIQSR
                     LPDPEWLEGWLDKQIQFDAVWENKVVERILHNMSLLL"
     gene            1574..2932
                     /locus_tag="VF453_17450"
     CDS             1574..2932
                     /locus_tag="VF453_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7689497.1"
                     /translation="MSRRRIAVVGAGISGLGAAWRLAPHCDVTVFEAEPRLGGHAHTV
                     DLALPGPDGAPVVHGVDTGFLVFNRRTYPRLVALFESLGIDCAASDMSFSVQAPRAGS
                     GRLEWAGTNLNAVFADRANLLRPSFHAMLLDLLRFNRLATALAQAGDDPALGQPLGEF
                     LAAHRFGDAFRDGYLLPMIGCIWSCPTAQMLRFPVGTLVRFCHNHGLLQLRERPQWYT
                     VRGGSRRYVERLAARVGRVRTGTPVLGVARDPGLRATHGGRIALRTAHGTESFDEVVL
                     ACHAPQALALLDDASLEERQVLGAMSTQDNEAVLHTDERLLPRRRAAWAAWNYEAGTA
                     GPGDHGVCLHYLINRLQPLPWRQPVIVSLNPLRAPRPETVLGRFRYAHPVFDHAAVAA
                     QARLGGLQGRSHVWFCGAWTGHGFHEDGLRSGLLAADHLLARLAPTVAGEPAAAALAE
                     AA"
     gene            3120..>3905
                     /locus_tag="VF453_17455"
     CDS             3120..>3905
                     /locus_tag="VF453_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HEX7689498.1"
                     /translation="MTAPAGARIAFGEVRHRRLAPVEHAFAYPTCFLLLPMRRLRGRA
                     DPVLARNRRAAFGFRDADHGDGGPDALAWFERLLARAGVRDADGEVWLHCLPRMLGFA
                     FKPVSFWYAHRADGSLAAVVAEVNNTFGERHCYLLEGDDLAFGRTQRARKVFHVSPFC
                     ETRGEYAFRFMRTDLGPGDGRGRATVRVELHVDGSPLIVTSVCGHVEPLTPGAVRRAL
                     WSLPAMTATIVARIHWQALRLWLRRVPFLSKPAPPATGLTRGTA"
BASE COUNT          489 a         1472 c         1368 g          576 t
ORIGIN      
        1 gagcagcagg ctcatgttgt gcaggatgcg ctcgacgacc ttgttctccc acacggcgtc
       61 gaactggatc tgcttgtcca gccagccctc gagccactcg gggtcgggca ggcggctctg
      121 gatggtgtcg cgcgggaaca gcttgacgtt gacgtgcagg ttggtcggat gcagcgcctg
      181 cgcggtgcgg cgcgcgctgg ccatcagcac gccgaccttg gagaacgcgg ccttcgcctc
      241 gtcgccgaag cgcatcagcg cgcgcttcat gtagcgcagg taggcgccgc cgtggcgggc
      301 ctcgtcgcgg gcgagcgtct cgtagatgtg cttgatcacc ggctccgtgt gccactgcgc
      361 ggcccggcgg taccagtggt tcagccggat ctccccgcag aagtgcagca tcagcgtctc
      421 gagcgcgggc gcgggatcga actcgaagcg cacggcgtgc agctcctctt ccgtcggcac
      481 gagctcggga cggaagcggc gcaggtactc catcagcacc agcgagtgct tctgctcctc
      541 gaagaaccag acgctcatga acgccgagaa gtccgagtcg tcgcggttgt cgcgcaggaa
      601 catctcggtg gccggcagcg ccgcccactc ggtgatggcg ttcatcttga tcgtcgtggc
      661 ctgctcgtcg gtcagcaggg acgggtcgaa ggactcccag gggatgtcct tgtccatgtc
      721 ccagcggacg gcctcgagtt gtttgaagag ttcgggatac agcatgctgg caagggcctc
      781 ctgacccgat tttacgcggc aggcaagcgt gtcgaagcgg cgaaacgcgc aagcataacc
      841 gccggattcc cgcaggaagt tcggcaacct gcagacccgg ggccggccgg cgccccgcgc
      901 ctgaccggaa ccgcggcgcg gcgggtgccg gcgagcccgt gccaggtgta cgaatcgccg
      961 cgccgcccgg atgccttcgg cgagcggccg ccaggccgcc gggggtgcgc cgcgcacgcg
     1021 ccgtcgcggc cgtgaatccg tttccacggt cgcggcgtat ccatgccacg cacgcaacga
     1081 tcctcgcgcg ccggcgaggc ccgcggcgac ccgccgggga ctgcgaccga ggcgcggaag
     1141 tcgccgttca tcccgccagg atgggcggcg aacaggaaaa attcacgaaa gaaggcccgc
     1201 tcatgccgat cttcgcccgg gacgatgccg ggacgtccgg aaccgtcgtc caacccgaca
     1261 gcaagcgctc acccgcagta acgcatgaaa cggaccctgt gcggcgcgga actttcggcg
     1321 cagaatccca tctcaacctg ggttccggtc gcgcatggcc ggttccccag caagactggt
     1381 tccgtttcat cgctgcgcag cttcgtcggg caaccggcgg aacgtcccga gcgcttctcc
     1441 tcctccctcc ctcccttgtt cgctcagggg cgctgcgcag cgatatttat cccggcccgg
     1501 ccgcggttcc gatgaagccg cggccccggc ccgcccttga cggccgtgcc cgcctcgccg
     1561 cggagcgcgc acgatgagcc gccgccgcat cgccgtcgtg ggtgcgggga tctccggcct
     1621 gggtgccgcc tggcgcctcg cgccgcactg cgacgtcacc gtcttcgagg cggagccccg
     1681 cctcggcggc catgcccaca ccgtggacct cgccctgccc ggcccggacg gcgcgccggt
     1741 cgtccacggc gtcgacaccg gcttcctcgt cttcaaccgc cgcacctacc cccggctcgt
     1801 cgcgctgttc gagtcgctgg gcatcgactg cgcggcgtcg gacatgtcgt tctcggtgca
     1861 ggcgccgcgc gccggctcgg gccgcctcga atgggccggc accaacctga acgccgtctt
     1921 cgccgaccgc gcgaacctgc tgcgcccgag cttccacgcg atgctgctgg acctgctgcg
     1981 cttcaaccgg ctcgcgaccg cgctggcgca ggcgggcgac gaccccgcgc tcggccagcc
     2041 cctgggcgag ttcctcgcgg cccaccgctt cggcgatgcc ttccgcgacg gctacctgct
     2101 gccgatgatc ggctgcatct ggtcgtgccc gacggcacag atgctgcggt tcccggtcgg
     2161 caccctggtg cgcttctgcc acaaccacgg cctgctgcag cttcgcgagc gcccgcagtg
     2221 gtacacggtg cgcggcggct cgcggcgcta cgtcgagcgg ctggccgcgc gcgtcggccg
     2281 cgtccgcacc ggcacgccgg tgctgggcgt cgcccgcgat ccgggcctgc gcgcgaccca
     2341 cggcgggcgc atcgcgctgc gcacggcgca cggcacggag tccttcgacg aggtcgtgct
     2401 cgcctgccac gccccgcagg cgctcgcgct gctggacgac gcgagcctcg aggagcgcca
     2461 ggtgctgggc gcgatgtcga cccaggacaa cgaggccgtc ctgcacaccg acgagcgcct
     2521 gctgccccgc cgccgcgccg cctgggcggc ctggaactac gaggccggaa ccgcgggccc
     2581 cggcgaccat ggcgtctgcc tgcactacct gatcaaccgc ctgcagccgc tgccgtggcg
     2641 gcagccggtg atcgtctcgc tcaacccgct gcgcgcgccg cggcccgaga ccgtgctggg
     2701 ccgcttccgc tacgcgcacc cggtgttcga ccacgccgcc gtcgccgcgc aggcgcgcct
     2761 gggcgggctg cagggacgct cgcacgtctg gttctgcggc gcctggacgg gccacggctt
     2821 ccacgaggac ggcctgcgct ccggcctgct cgccgccgac cacctgctcg cgcgcctggc
     2881 gccgacggtc gcgggcgaac cggcggcggc ggcgctggcg gaggcggcat gagccgtcgc
     2941 ccggccgttc cgaagagggc tcgggccgga gtccgcagga cgaaggcttc ctggtccgct
     3001 cgcccggccg ctccgaagag ggctcgcgcc ggagtccgca ggacggaggc ttcctggttc
     3061 gctcgctcgg ccgctccgaa gagggctcgc gccgcagtgc acggcccgga ggctgcctcg
     3121 tgacggcacc ggcaggcgcc cggatcgcgt ttggcgaggt gcgccatcgc cggctcgcgc
     3181 cggtcgagca cgcctttgcc tatccgacct gcttcctgct gctgccgatg cgccgcctgc
     3241 ggggacgcgc cgaccccgtg ctcgcccgca accggcgcgc cgccttcggc ttccgcgacg
     3301 ccgaccacgg cgacggcggc ccggacgcgc tggcctggtt cgagcgcctg ctcgcgcgcg
     3361 cgggcgtgcg cgatgccgac ggcgaggtct ggctccattg cctgccgcgc atgctcggct
     3421 tcgccttcaa gccggtgagc ttctggtacg cgcaccgcgc ggacggctcg ctcgccgcgg
     3481 tcgtcgccga ggtcaacaac accttcggcg agcgtcactg ctacctgctc gagggcgacg
     3541 acctggcctt cggccgcacg caacgcgcgc gcaaggtctt ccacgtgtcg ccgttctgcg
     3601 agacgcgggg cgagtacgcc ttccgcttca tgcgcaccga cctcggccct ggcgacggcc
     3661 gcgggcgcgc gacggtgcgc gtcgagctgc atgtcgacgg ctcgccgctg atcgtcacca
     3721 gcgtctgcgg gcacgtggag ccgctgacgc ccggcgcggt gcggcgggcg ctgtggtcgc
     3781 tgcccgcgat gacggccacg atcgtcgccc gcatccactg gcaggcgctg cggctgtggc
     3841 tgcggcgcgt gcccttcctg tccaagcccg cgccgccggc gaccggcctg acgcgcggga
     3901 cggcc
//
LOCUS       DASVLN010000736         8856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_469726, whole genome shotgun sequence.
ACCESSION   DASVLN010000736 DASVLN010000000
VERSION     DASVLN010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469726"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VF453_17460"
     CDS             complement(<1..870)
                     /locus_tag="VF453_17460"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX7689499.1"
                     /translation="MLRRLRYIAGSLKARLALAGVVLIAASVALTLVLVLHAMERRSQ
                     RAVLDAELANAERIAVVLSTKLVSLQTALRQAARGVPADRLDDPAALTRYLAGQGVLR
                     SLFDSVFIASAQGRLVAVADSRGIRPIAIDASDRNYVRRTLAERRSVVSQPGVSRTDG
                     VSMFVITMPVTDRDGTVRAILVCGTRLSTNQLVDDLTRPSSGGHDPVTTVVTDAGGLV
                     ISHPDGGRVLHPLAADPRFERVVAAWRAQGAPIDPQGSSARLGDDIVAFAGVPDADWV
                     VFRSAPAELLLGGP"
     gene            complement(890..1651)
                     /locus_tag="VF453_17465"
     CDS             complement(890..1651)
                     /locus_tag="VF453_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEX7689500.1"
                     /translation="MFEDTRPAPLDAMGSDELLADFRVVDAGEIRALLKALMDQVVPL
                     NLSASDGSAYTTTLWTVDPNAGRISFTADTMAHAVHDIVEADECAAVAYLDKIKVQFD
                     VQDLVLVQGARTSVLQARLPRELFRFQRRNSYRVRTIERTAPTASFRHPGIPEMGVSL
                     RVLDVSVGGCALLVPADVPPLQPGMTVRGVRLALDAETRVDCALVLHHVTSTGAENGS
                     VRLGCGIVGVDGGAERALQKYIDQTQKRRRMMALD"
     gene            complement(1720..2019)
                     /locus_tag="VF453_17470"
     CDS             complement(1720..2019)
                     /locus_tag="VF453_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HEX7689501.1"
                     /translation="MSTTLLNYYEAIEKASAEMLEAARAGNWDTVVKLEGACAVLIAQ
                     LKNAAQTSKLQQAEAKEKSRIMQRILLNDAEIRHLAEPWLEDLDQMLGGRPAVLH"
     gene            complement(2061..2498)
                     /gene="fliS"
                     /locus_tag="VF453_17475"
     CDS             complement(2061..2498)
                     /gene="fliS"
                     /locus_tag="VF453_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEX7689502.1"
                     /translation="MFTSAYAPSTFGRAQGFAQAYARIGAETSVNSASAHHLITLLFN
                     GFIDSVTLARGAMREGRIEAKGKAIGRAARIVEEGLKAGLNMQEGGKLAADLSALYAY
                     VALRLTVANLRNDESALDECVRLINPLREAWISIGPQVEAGNL"
     gene            complement(2631..4127)
                     /gene="fliD"
                     /locus_tag="VF453_17480"
     CDS             complement(2631..4127)
                     /gene="fliD"
                     /locus_tag="VF453_17480"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEX7689503.1"
                     /translation="MTTSSISSTGSISSAGIGSGLDVNGIISSLMAVESQPLTLLQNK
                     ASTINTEISAVGQIQSLASALGDKAQALASPGLWSQTTSSSADPTVVTADTSSGNAVA
                     GDYQVSVQQLAQGQTVSTGTPLASTTSTLDTGSITIQLGTWTAGTGGASPTFAAASSG
                     PLTISIGAGETSLAAIRDKINAANAGVNATIITDATGARLSLRSTATGAAAGFKISTT
                     EDTPAADPSQGLAALAYDPSAGGTGGMTLNQSAANALATVNGISVVSASNTLTNISDG
                     LNLTLNKVSATPVDVSVSIDTASMQKAITDFATAYSSLNNMIYQDTKYDIGTAPAAGT
                     ARKDSPLQGDATIVGFQNQLRAIINTTSTTSSVFARLSDIGVAVQTDGSLQVNSSKLT
                     AALQKPAELKKLFATQGTNDANTGIAVRFSNLVDAVQAPDGALSTHVAGMRTELTYNQ
                     KQQDDMQTHLDATQARLTAQYQALDTTMSKMNALSSYVTQQITMMQKA"
     gene            complement(4368..5216)
                     /locus_tag="VF453_17485"
     CDS             complement(4368..5216)
                     /locus_tag="VF453_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin domain-containing protein"
                     /protein_id="HEX7689504.1"
                     /translation="MPMTINTNIASLNAQRNLNASQGSLATSMQRLSSGLRVNSAKDD
                     AAGLAIAERMNSQVRGMNVAVRNANDGISMAQTAEGALAQVSNSLQRMRELAVQARNS
                     TNSSSDKDSLNKEFSQLQAEITRVLGGTTFNGKHILGSDATSLTFQIGANTTADDTIS
                     VTTSNMTQDTTITTITASTAVIDASADATAIGAVINNIDAAIDGVNDTRATFGATQSR
                     FDAIITNLQTSVENQSAARSRIMDADFAAETANMSRAQVLQQAGTAMVAQANQMPQQV
                     LKLLQG"
     gene            complement(5598..6443)
                     /locus_tag="VF453_17490"
     CDS             complement(5598..6443)
                     /locus_tag="VF453_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX7689505.1"
                     /translation="MTLSINTNIASLDAQRNLAASQSSLSTSMQRLSSGLRINSAKDD
                     AAGLAIAERMNSQVRGMNVAIRNSNDGISLAQTADGALAQVGNSLQRMRELAVQARNS
                     TNSSSDKDSLNKEFSQLQAEINRVLGGTTFNGKAMLGADATKMDFQVGANTTSNDTVS
                     ITTTDMTQDSKITAVTGSSAVIDSSADASAIGTVIDNIDKAIDDVNDVRATFGASESR
                     FDSIISNLQSSVENQSAARSRIMDADFASETANMSRAQVLQQAGTAMIAQANQQPQQV
                     LTLLR"
     gene            complement(6891..7736)
                     /locus_tag="VF453_17495"
     CDS             complement(6891..7736)
                     /locus_tag="VF453_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin domain-containing protein"
                     /protein_id="HEX7689506.1"
                     /translation="MTLSINTNIASLDAQRNLSTSQSSLATSMQRLSSGLRINSAKDD
                     AAGLAIAQRMQAQVRGMNVAIRNSNDGISLSQTADGALAQVGDSLQRMRELAVQARNS
                     TNNTSDKDSLNKEFSQLQQEITRVLGGTTFNGKAMLGSDATKMDFQVGANTTSNDTVS
                     ITTTDMTKDSKITAVTGSSAVIDHTADASAIGTVIDNIDKAIDDVNDVRATFGASESR
                     FDSIISNLQSSTENQSAAESRIMDADFAAETANMSRAQVLQQAGTAMVAQANQLPQQV
                     LTLLR"
     gene            8056..>8856
                     /locus_tag="VF453_17500"
     CDS             8056..>8856
                     /locus_tag="VF453_17500"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7689507.1"
                     /translation="MQNLSSPDRQPRRSPGARRAESHRQAGLKAQRTQAWGLAEREFQ
                     RAADLAPEDALMWLNLARTRVQLGKHVSGLEAARRAVELDRADPVACRMAADLLLQMS
                     RPAEALAILEGLAPEAPRDHDFHNSHGNALFQCKKPREAVDAFFKALALKVDAAIVHY
                     RLGLCFMDMAMALEAAECFRTAISLDDGAVRALALSLIVHESRQACDWSRIDDDTRAL
                     LDAVEGEDAEVGRLLSPFALLAIDATPAQQRRMGARRLAGLTRRVVPLP"
BASE COUNT         1169 a         2912 c         3223 g         1552 t
ORIGIN      
        1 cgggccgccc agcagcagct ccgcgggcgc gctgcggaag acgacccagt cggcatcggg
       61 caccccggcg aacgccacga tgtcgtcgcc caggcgcgcg gacgagccct gcgggtcgat
      121 cggcgcgcct tgcgcccgcc acgccgcgac cacgcgctcg aagcgcgggt cggcggccag
      181 gggatgcagc acgcgcccgc cgtcgggatg cgagatcacc agcccgcccg cgtcggtcac
      241 gaccgtggtg accggatcgt ggccgccgct ggacggccgc gtgaggtcgt ccaccagctg
      301 gttggtcgac agccgcgtgc cgcacacgag gatcgcccgc accgtgccgt cgcggtcggt
      361 cacgggcatc gtgatgacga acatcgagac gccgtccgtg cggctgacgc ccggttgcga
      421 gacgaccgag cgccgctcgg ccagcgtgcg gcggacgtag ttgcggtcgg aggcgtcgat
      481 cgcgatcggc cggatgcccc tcgagtccgc caccgcgacc aggcgcccct gcgccgacgc
      541 gatgaagacg ctgtcgaaca ggctgcgcag cacgccctgg ccggcgaggt agcgggtcag
      601 ggcggcgggg tcgtcgaggc ggtcggccgg cacgccgcgc gcggcctggc gcagcgcggt
      661 ctgcagcgag accagcttgg tggacagcac gaccgcgatg cgctcggcgt tggcgagctc
      721 ggcgtcgagc accgcgcgct ggctgcgccg ctccatcgcg tgcagcacca gcacgagcgt
      781 cagcgcgacg ctcgcggcga tcagcaccac gcccgcgagc gccaggcgcg ccttgagcga
      841 gccggcgatg tagcgcaacc gccgcagcat gcaaccgccg gcgggatcct cagtcgagcg
      901 ccatcatgcg gcgccgcttc tgggtctggt cgatgtactt ctgcagcgcg cgctcggcgc
      961 cgccgtcgac gcccacgatg ccgcagccca ggcgcaccga gccgttctcg gcgcccgtgg
     1021 aggtgacgtg gtgcagcacg agcgcgcagt cgacgcgggt ctcggcgtcg agcgcaaggc
     1081 gcacgccgcg cacggtcatg cccggctgca gcggcggcac gtcggccggc accagcagcg
     1141 cgcagccgcc gacgctcacg tcgaggacgc gcagcgagac gcccatctcc gggatgcccg
     1201 ggtggcggaa gctcgcggtc ggcgccgtgc gctcgatcgt gcgcacgcgg taggagttgc
     1261 ggcgctggaa gcggaacagc tcgcgcggca ggcgcgcctg cagcacgctg gtgcgcgcgc
     1321 cctgcaccag cacgagatcc tggacgtcga actgcacctt gatcttgtcg aggtaggcga
     1381 cggccgcgca ctcgtcggcc tcgacgatgt cgtgcacggc gtgcgccatc gtgtcggcgg
     1441 tgaacgagat gcggcccgcg ttcgggtcga ccgtccacag cgtcgtggtg taggcgctgc
     1501 cgtcggacgc gctgaggttc agcggcacga cctggtccat cagcgccttc agcagcgcgc
     1561 ggatctcccc ggcgtcgacg acgcggaaat cggcgaggag ctcgtcggag cccatcgcgt
     1621 cgaggggagc gggtcgggtg tcttcgaaca tcgcggtggt cttcgttgtc gtcccttgtc
     1681 gagggccggg gccggggggc cggccgcggc cggcgatcgt caatgcagga ccgcgggacg
     1741 gccgcccagc atctggtcga gatcctcgag ccagggctcg gcgaggtggc ggatctccgc
     1801 gtcgttcagg aggatgcgct gcatgatgcg cgacttctcc ttggcctcgg cctgttgcag
     1861 cttgctggtc tgcgcggcgt tcttcagctg cgcgatgagc accgcgcagg caccctccag
     1921 cttcaccacc gtgtcccagt tgccggcgcg ggcggcctcg agcatctcgg cgctggcctt
     1981 ctcgatcgcc tcatagtaat tgagcagcgt ggtactcatg atgttgtccg tgatgggagt
     2041 gcccgaaata ttcgaaatgt tcacagattg cccgcttcga cctgcggccc gatgctgatc
     2101 caggcttcgc gcagggggtt gatcaggcgc acgcattcgt ccagggcgga ctcgtcgttg
     2161 cgcaggttgg cgacggtcag gcgcagcgcc acgtaggcgt agagcgccga cagatccgcg
     2221 gccagcttgc caccttcctg catgttgagg ccggccttca ggccttcctc cacgatccgc
     2281 gcggcccggc cgatggcctt gcccttcgcc tcgatgcggc cttcgcgcat cgcgccgcgc
     2341 gcgagcgtca ccgagtcgat gaagccgttg aacagcaacg tgatgagatg gtgcgccgag
     2401 gcggagttga cgctggtctc cgcgccgatc cgcgcgtacg cctgggcgaa gccctgggcg
     2461 cgaccgaagg tggagggagc gtaggcagag gtgaacatgg tatcgagctg ggatccgtga
     2521 tccgtctgag tgttcgtctt gacttgttat cgggcgtccg cggccgggtc ttgaacgacg
     2581 aactgtccgt tttccgccgc cttagccgcc tccagtcggc gcgggccgcg tcaggccttc
     2641 tgcatcatcg tgatctgctg cgtgacgtag ctcgagagcg cgttcatctt cgacatcgtg
     2701 gtgtcgagcg cctggtactg cgccgtcagg cgggcctgcg tcgcgtccag gtgggtctgc
     2761 atgtcgtcct gctgcttctg gttgtaggtc agctccgtgc gcatgcccgc gacgtgcgtg
     2821 gagagcgcgc cgtccggcgc ctgcaccgcg tcgaccaggt tcgagaagcg caccgcgatg
     2881 cccgtgttcg cgtcgttcgt gccctgggtc gcgaacagct tcttgagctc cgccggcttc
     2941 tgcagcgccg cggtgagctt gctggagttc acctgcagcg agccgtccgt ctgcaccgcc
     3001 acgccgatgt ccgacaggcg cgcgaacacc gacgaggtgg tggacgtcgt gttgatgatc
     3061 gcgcgcagct ggttctggaa gccgacgatc gtcgcgtcgc cctgcagcgg gctgtccttg
     3121 cgcgcggtgc cggcggccgg cgccgtgccg atgtcgtact tggtgtcctg gtagatcatg
     3181 ttgttcaacg acgagtacgc cgtcgcgaag tcggtgatcg ccttctgcat ggaggccgtg
     3241 tcgatcgaca ccgagacgtc caccggcgtc gcgctgacct tgttgagcgt caggttcagg
     3301 ccgtcggaaa tgttggtcag cgtgttcgac gcggagacca ccgagatgcc gttgacggtc
     3361 gcgagcgcgt tggccgcgct ctggttgagc gtcatgccgc cggtgccgcc cgcgctcggg
     3421 tcgtaggcga gcgcggccag gccctggctc gggtcggcgg ccggcgtgtc ctcggtcgtc
     3481 gagatcttga agccggcggc ggcgccggtg gcggtcgagc gcagcgacag ccgggcgccc
     3541 gtggcgtcgg tgatgatggt cgcgttcacg ccggcgttgg cggcgttgat cttgtcgcgg
     3601 atcgcggcga gcgaggtctc gcccgcgccg atgctgatcg tcagcggacc ggagctcgcg
     3661 gccgcgaagg tcggcgaagc gccgccggtg ccggcggtcc aggtgccgag ctggatcgtg
     3721 atcgacccgg tgtccagcgt cgaggtcgtg gacgcgagcg gcgtgcccgt gctcaccgtc
     3781 tgtccctgcg cgagctgctg cacgctgacc tggtagtcgc cggccaccgc gttgccgctg
     3841 gaggtgtcgg ccgtcacgac ggtcggatcc gcggagctgg acgtcgtctg cgaccacagg
     3901 ccgggcgagg cgagcgcctg cgccttgtcg ccgagcgccg aggcgaggct ctggatctgg
     3961 ccgaccgcgg agatctccgt gttgatcgtc gaggccttgt tctgcagcag cgtgagcggc
     4021 tggctctcga cggccatcag cgaggagatg atgccgttga cgtccaggcc gctgccgatg
     4081 ccggcggaac tgatggagcc ggtggagctg atggagctgg tcgtcatggt ctttccgggc
     4141 ggttcttgag cggtctatcg gcagcccggc gccgggcttg agcgaatcct ggcggccggg
     4201 cgcgcgccgt tcgagcgaca agccgccggc gcgcgaggct gcaaatgcgg cggcgcgctg
     4261 cggctgccgg cgggaagcag ggctttcccg ggggcagtcg cagcgcgccg atgggggacg
     4321 acggacgtcc gggggacggc ggcgaggcgc gcgcgcctcg ccggtcgtca gccctggagc
     4381 agcttgagca cctgctgcgg catctggttg gcctgcgcca ccatcgccgt gccggcctgc
     4441 tgcagcacct gggcgcggct catgttggcc gtttccgccg cgaagtcggc atccatgatg
     4501 cgcgagcggg cggccgactg gttctcgacc gaggtctgca ggttggtgat gatcgcgtcg
     4561 aagcggctct gggtggcgcc gaaggtcgcg cgggtgtcgt tgacgccgtc gatcgcggcg
     4621 tcgatgttgt tgatgacggc accgatcgcg gtcgcgtcgg cggaggcgtc gatgacggcg
     4681 gtcgaggcgg tgatcgtcgt gatcgtcgtg tcctgcgtca tgttggacgt cgtcaccgag
     4741 atcgtgtcgt cggccgtcgt gttcgccccg atctggaagg tcagcgaggt cgcgtcggag
     4801 ccgaggatgt gcttgccgtt gaaggtcgtg ccgcccagca cccgcgtgat ttccgcctgc
     4861 agctgcgaga actccttgtt cagcgagtcc ttgtccgacg agctgttggt cgagttgcgg
     4921 gcctgcaccg ccagctcgcg catgcgctgc agcgagttgc tcacctgcgc cagcgcgcct
     4981 tcggccgtct gcgccatcga gatgccgtcg ttggcgttgc gcaccgcgac gttcatgccg
     5041 cgcacctggg agttcatgcg ctccgcgatc gcgaggccgg cggcgtcgtc cttcgcggag
     5101 ttcacgcgca ggcccgacga caggcgctgc atcgacgtgg cgagcgagcc ttgcgaggcg
     5161 ttgaggtttc gctgcgcgtt cagcgaagcg atgttggtgt tgatggtcat tggcatggca
     5221 ttactccagg actgaaggca caaccggcga aagtgccgga actcagttcg atgcgaagct
     5281 gtcaggttgg ccccacggcc gcgcacctcc aagcccggta cttggtcacc acggtggccg
     5341 gcgtcgtcgc ctgcgcccgc tagtgcttcc ggaccgcgtc ccttcccggt cttgcggtcg
     5401 ggaggagacg ttgatcggat tgtgcggaga cttgagggcc gccaaatcgc ggatatgcgc
     5461 cgccaagccg gttcaattcc cgcctgtccg gctgcccgtc ccgggcgccg gccggaaatg
     5521 gaaagacccc gccggttcgc accgggcggg gtcgtctgga gtgcctgggg aaggcggcct
     5581 gccggccgcc ggcgccgtca gcgcaggagc gtcagcacct gctgcggctg ctggttggcc
     5641 tgggcgatca tcgccgtgcc ggcctgctgc agcacctggg cgcggctcat gttcgcggtt
     5701 tccgacgcga agtcggcgtc catgatgcgg ctgcgggcgg ccgactggtt ctcgacggag
     5761 ctctgcaggt tcgagatgat cgagtcgaag cggctctccg aggcaccgaa ggtggcgcgc
     5821 acgtcgttga cgtcgtcgat ggccttgtcg atgttgtcga tgaccgtgcc gatcgcgctg
     5881 gcgtcggccg aggagtcgat gaccgccgag gagccggtca ccgcggtgat cttgctgtcc
     5941 tgggtcatgt cggtcgtcgt gatcgacacc gtgtcgttgc tcgtcgtgtt ggcgccgacc
     6001 tggaagtcca tcttggtcgc atcggcgccc agcatggcct tgccgttgaa ggtcgtgccg
     6061 ccgagcacgc ggttgatttc cgcctggagc tgcgagaact ccttgttcag cgaatccttg
     6121 tccgacgagc tgttggtcga gttgcgcgcc tgcaccgcga gctcgcgcat gcgctgcagc
     6181 gagttgccca cctgggccag cgcgccgtcg gcggtctggg ccagcgagat gccgtcgttg
     6241 gagttgcgga tcgcgacgtt catgccgcgc acctgcgagt tcatgcgctc ggcgatggcc
     6301 aggccggcgg cgtcgtcctt ggccgagttg atgcgcaggc ccgaggacag gcgctgcatc
     6361 gaggtcgaca gcgaggactg cgatgcggcg aggttgcgtt gcgcgtccag cgaggcgatg
     6421 ttggtgttga tcgagagagt catgatgttt ccaatgcaag aagacgaaga attacgaaga
     6481 gcgtctccgg gaagagcacc ggcgaattcc ttcaccaggc gccaggtgct ccttcgagcc
     6541 ggacggcgcc ctgcgcaccg actgaccctt ggtcactcac cggacccaag ctcgatttcg
     6601 tgagcctgtt ggtttggata tcgggggagc cccgagaaac ctttaggcct tttttgaccg
     6661 acgcccccgg cgatgcttgc tcgccggggg tgccaggttg tcgtcccgat tccgggaaac
     6721 tgaaactcgt cttcgtcttc gtcttcgtct ggaaaccgga ctctcacgtt caggatcttc
     6781 gttctcgtcc gcccttggca gggatcgggc cgacggttcg tccgaacgtc tcgcgacgtc
     6841 ttcccggtca ggcggtcagc cccgcgtcgg cggcggggct gccgccggga tcagcgcagc
     6901 agggtcagca cctgctgggg cagctggttc gcctgcgcga ccatcgccgt gccggcctgc
     6961 tgcagcacct gggcgcggct catgttcgcg gtttccgcgg cgaagtcggc gtccatgatg
     7021 cggctctcgg cggccgactg gttctcggtg ctcgactgca ggttcgagat gatggagtcg
     7081 aagcggctct ccgaggcacc gaaggtggcg cgcacgtcgt tgacgtcgtc gatggccttg
     7141 tcgatgttgt cgatcacggt gccgatggcg ctcgcgtcgg ccgtgtggtc gatgaccgcc
     7201 gaggagccgg tcaccgcggt gatcttgctg tccttggtca tgtcggtcgt cgtgatcgag
     7261 accgtgtcgt tgctggtcgt gttggcgccg acctggaagt ccatcttggt cgcgtccgag
     7321 ccgagcatcg ccttgccgtt gaaggtcgtg ccgccgagca cgcgggtgat ttcctgctgc
     7381 agctgcgaga actccttgtt cagcgagtcc ttgtcggacg tgttgttggt cgagttgcgg
     7441 gcctgcacgg cgagttcacg catgcgctgc agcgagtcgc ccacctgcgc cagcgcgccg
     7501 tccgcggtct gggacagcga gatgccgtcg ttggagttgc ggatcgcgac gttcatgccg
     7561 cgcacctgcg cctgcatgcg ctgggcgatc gccaggccgg cggcgtcgtc cttggccgag
     7621 ttgatgcgca ggcccgagga caggcgctgc atcgaggtgg ccagcgacga ctgcgacgtc
     7681 gacaggttgc gttgggcgtc cagcgacgcg atgttggtgt tgatcgagag ggtcatgctt
     7741 cactcctgtg gagacaaaat ccgggtaacc ccggaggttt cgccccagag tcgaaaacgg
     7801 gtggggccgt gagaaggttt tcgagcctcg atccgattac ttgagggctt ttttcagccc
     7861 ttttctcgcg atcgagcgcg ggcgatccgg cgcaggcgtc gtcaggtgcg gtccattgcc
     7921 cggccgtgac ggcggctccg cgcgccaagc cgaagcgccg caggcccgtc gctcggccgg
     7981 ttggcggaac tgggcggggg aacgcccggt tatccgaccg tcgggaacgc gctgccttcc
     8041 tagactggtc cgatcatgca gaacctctcg tccccggacc gccagccccg gcgctcgccc
     8101 ggcgcccggc gcgccgaaag ccaccgccag gccggcctga aggcgcagcg cacgcaggcc
     8161 tggggcctgg ccgagcgcga gttccagcgc gccgccgacc tcgcgcccga ggacgcgctg
     8221 atgtggctga acctcgcgcg cacgcgcgtg cagctgggca agcacgtgtc cgggctcgag
     8281 gcggcccggc gcgccgtcga gctcgaccgc gccgacccgg tggcctgccg catggccgcc
     8341 gacctgctcc tgcagatgtc gcgccccgcc gaggcgctcg cgatcctgga gggcctcgcc
     8401 cccgaggcgc cgcgtgacca cgacttccac aattcccatg gcaacgcgct gttccagtgc
     8461 aagaagccgc gcgaggccgt cgacgcgttc ttcaaggcgc tcgcgctgaa ggtcgacgcc
     8521 gcgatcgtcc actaccggct cggcctgtgc ttcatggaca tggcgatggc gctggaggcc
     8581 gcggaatgct tccgcacggc gatctcgctg gacgacggcg ccgtgcgcgc gctcgcgctg
     8641 agcctgatcg tccacgagag ccggcaggcg tgcgactggt cgcgcatcga cgacgacacc
     8701 cgcgcgctgc tggacgccgt cgagggcgag gacgccgagg tcggccggct gctgtcgccg
     8761 ttcgcgctgc tggccatcga cgccacgccc gcccagcagc ggcgcatggg cgcgcgccgc
     8821 ctcgccggcc tgacgcgtcg cgtcgtgccg ctgccg
//
LOCUS       DASVLN010000737         2743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_522868, whole genome shotgun sequence.
ACCESSION   DASVLN010000737 DASVLN010000000
VERSION     DASVLN010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522868"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VF453_17505"
     CDS             complement(<1..907)
                     /locus_tag="VF453_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7689508.1"
                     /translation="MIVRHTFSPLDNERLAHLCGALDENLRAIEQGLNVVVTRRHEHF
                     RVEGGKLQSARAMALIEALYERSGRAIDEEQLQLMLHDAQRDVIQGHAKPVPPEEVAA
                     GGEIVLHTRRANLIGRTPNQVQYLRQILSHDITIGVGPAGTGKTFLAVACAVDALERS
                     SVQRIVLTRPAVEAGERLGFLPGDLSQKVDPYLRPLYDALYDLMGFERVSKAFERGAI
                     EIAPLAFMRGRTLNHAFVILDEAQNTTPEQMKMFLTRIGFGTKAVVTGDLSQVDLPRG
                     ARSGLADASEVLREVAGIAATRFGAA"
     gene            1004..1576
                     /gene="ruvA"
                     /locus_tag="VF453_17510"
     CDS             1004..1576
                     /gene="ruvA"
                     /locus_tag="VF453_17510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7689509.1"
                     /translation="MIGRLTGIVAEKSPPLVLLDVQGVGYEVDVPMSTFCNLPALGER
                     TTLHTHLVVREDAHQLFAFLTEEERATFRLLIRISGIGPRTALAILSGLSVQDLAGAV
                     SRQEGGRLQKVPGIGKKTADRLLLELKGKLGPDLGAGPIAVASDAQGDIVQALIALGY
                     NEKEAALAIKGLPKDVGVSDGIKLAMKALS"
     gene            1614..2702
                     /gene="ruvB"
                     /locus_tag="VF453_17515"
     CDS             1614..2702
                     /gene="ruvB"
                     /locus_tag="VF453_17515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7689510.1"
                     /translation="MSIQTDDFAPAGSGRPARVVAAGPASPNEEALERALRPKRLDDY
                     VGQAKAREQLDIFIGAARKRAEALDHVLLFGPPGLGKTTLSHIIAAELGVNLRQTSGP
                     VLEKPKDLAAILTGLERNDVLFIDEIHRLSPVVEEILYPALEDFQIDIMIGDGPAARS
                     IKLDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYDASELARIVRRSAGLLNAPID
                     DAGALEIARRSRGTPRIANRLLRRVRDYADVKAEGRISAEVADKALAMLDVDPQGFDV
                     MDRKLLEAIVHRFDGGPVGLDNVAAAIGEESGTIEDVIEPYLIQQGYLQRTPRGRIAT
                     LAAYRHLDLAPPPRAGADLFGAMPPEAS"
BASE COUNT          395 a          979 c          985 g          384 t
ORIGIN      
        1 cggccgcgcc gaagcgcgtg gccgcgatgc cggcgacctc gcgcagcacc tcgctcgcgt
       61 cggccagccc gctgcgcgcg ccgcgcggca ggtcgacctg cgacaggtcg cccgtgacca
      121 cggccttcgt gccgaagccg atgcgcgtga ggaacatctt catctgctcc ggcgtcgtgt
      181 tctgcgcctc gtctaggatg acgaatgcat ggttgagggt gcgcccgcgc atgaaggcga
      241 gcggcgcgat ctcgatggcg ccgcgctcga aggccttgct gacgcgctcg aagcccatga
      301 ggtcgtagag cgcgtcgtac agcgggcgca ggtaggggtc gaccttctgc gacaggtcgc
      361 ccggcaggaa gcccaggcgc tcgccggcct cgacggcggg ccgcgtcagc acgatgcgct
      421 ggacgctgct gcgctccagg gcgtcgaccg cgcaggccac cgcgaggaag gtcttgccgg
      481 tgccggccgg gccgacgccg atcgtgatgt cgtgcgacag gatctgccgc aggtactgca
      541 cctggttggg cgtgcggccg atcaggttgg cgcggcgcgt gtgcagcacg atctcgccgc
      601 cggcggcgac ctcctcgggc ggcaccggct tcgcgtgtcc ctggatcacg tcgcgctgcg
      661 cgtcgtgcag catcagctgc agctgctcct cgtcgatggc gcggccgctg cgctcgtaca
      721 gcgcctcgat caacgccatc gcgcgggccg actgcagctt gccgccctcg acgcggaaat
      781 gctcatggcg gcgcgtgacg acgacgttga gcccctgctc gatcgcgcgc aggttctcgt
      841 cgagcgcgcc gcacaggtgg gcgaggcgct cgttgtcgag gggggagaac gtgtggcgga
      901 cgatcaaggc ggtgcggcgg ttgcgacgac gggaagtcgc gcattgtccg ccagcgcggc
      961 gatccgtgcc ggccctgcgc cggcgcgccc gataatcgcg cgcatgatcg gtcgtctcac
     1021 gggcatcgtc gcggaaaagt cgcccccgct tgtgctgctg gacgtgcagg gcgtcggcta
     1081 cgaggtcgac gtgcccatga gcaccttctg caacctgccg gcgctgggcg agcgcacgac
     1141 gctgcacacg cacctggtcg tgcgcgagga cgcgcaccag ctgttcgcct tcctgaccga
     1201 ggaggagcgc gcgaccttcc gcctgctgat caggatctcg ggcatcgggc cgcggacggc
     1261 gctggcgatc ctgtcggggc tgtcggtgca ggatctcgcg ggggccgtct cgcgccagga
     1321 gggtggccgg ctgcagaagg tgcccggcat cggcaagaag accgccgacc gcctgctgct
     1381 ggagctgaag ggcaagctcg gcccggacct cggcgccggc cccatcgccg tggcgagcga
     1441 cgcccagggc gacatcgtgc aggcgctgat cgccctcggc tacaacgaga aggaagcggc
     1501 gctcgcgatc aagggcctgc ccaaggacgt cggcgtgagc gacggcatca agctggcgat
     1561 gaaagcgctg tcctgaacgc cgcatgaagc ctcgcgcgag cgataatcac tgaatgtcca
     1621 tacagaccga cgatttcgcc cccgccggct ccggccgccc ggcgcgcgtc gtcgcggccg
     1681 ggcccgcctc gcccaacgag gaggccctgg agcgcgcgct gcgcccgaag cggctcgacg
     1741 actacgtcgg ccaggcgaag gcgcgcgagc agctggacat cttcatcggc gcggcccgca
     1801 agcgcgcgga ggcactcgac cacgtgctgt tgttcggccc gccggggctg ggcaagacca
     1861 cgctgagcca catcatcgcc gcggagctgg gcgtgaacct gcgccagacc tcggggccgg
     1921 tgctggaaaa gccgaaggac ctcgccgcca tcctcaccgg gctggagcgc aacgacgtgc
     1981 tcttcatcga cgagatccac cgcctgtcgc cggtcgtcga ggaaatcctg tatcccgcgc
     2041 tggaggactt ccagatcgac atcatgatcg gcgacggccc ggccgcgcgc tcgatcaagc
     2101 tcgacctgca gccgttcacg ctcgtcggcg ccaccacccg ggcgggcatg ctgaccaatc
     2161 cgttgcgcga ccgcttcggc atcgtcgcgc gcctggagtt ctacgacgcg agcgagctcg
     2221 cgcgcatcgt ccgccgctcc gccggcctgc tgaacgcgcc gatcgacgac gccggggcgc
     2281 tggagatcgc ccgccgctcg cgcgggacgc cgcgcatcgc caaccgcctg ctgcgccgcg
     2341 tgcgcgacta tgccgatgtg aaggcggagg gccgcatcag cgccgaggtc gccgacaagg
     2401 cgctcgcgat gctggacgtc gacccgcagg gcttcgacgt gatggaccgc aagctgctcg
     2461 aggccatcgt ccaccgcttc gacggcggcc cggtcggcct ggacaacgtc gccgccgcga
     2521 tcggcgagga gtccggcacg atcgaggacg tcatcgagcc ctacctgatc cagcagggct
     2581 acctgcagcg cacgccgcgc ggccgcatcg cgacgctggc ggcctaccgc cacctcgacc
     2641 tcgcgccgcc gccgcgggcg ggcgcggacc tgttcggcgc gatgccgccc gaggcgagct
     2701 gaggcgtcgc gttcaggcgc cgaccgggtc tgcggccacg tgc
//
LOCUS       DASVLN010000738         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_199518, whole genome shotgun sequence.
ACCESSION   DASVLN010000738 DASVLN010000000
VERSION     DASVLN010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199518"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF453_17520"
     CDS             <1..231
                     /locus_tag="VF453_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HEX7689511.1"
                     /translation="ARLRARAARGAWPSSPAAPDDGPPELISDYGATDPAEFFAVVTE
                     VFYEQPAALAVRHPALFEVLRGYYRVDPREWH"
     gene            364..1086
                     /locus_tag="VF453_17525"
     CDS             364..1086
                     /locus_tag="VF453_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7689512.1"
                     /translation="MLTLRKASDRGHADHGWLSSHHSFSFADYHDPAHMGFGNLRVLN
                     DDTVAPGTGFGTHGHRDMEIVSYVLDGALAHADSMGNGQPGGAHAGVIVPGDVQRMTA
                     GTGVRHSEFNHSKTQPTHFLQIWLLPERLGLAPGYEQVNVPAASKRGRLALIAAPQAA
                     PGAVRLNADARILAGLFDGDERQSLPLPAGRLAYVHVARGALRVNGQALEAGDALKVR
                     DEAELVLEDGRGAEVLVFDLAP"
     gene            1242..2501
                     /locus_tag="VF453_17530"
     CDS             1242..2501
                     /locus_tag="VF453_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEX7689513.1"
                     /translation="MSDTAPRRHAPTTRLLHADRLAGVEAGATHKPLHVSAAFNYPSA
                     RDLCEVFQGNQPGHVYARQGNPTVQALEAKVAMLEDAKGCAAFATGMAAVAAVFLALL
                     RSGDHVVCTRFLFGNTNSLMQTLQGLGVAVTFVDATDAANVRAALTPATRLVFVETIA
                     NPVTQVADLAAVGDACREAGVLYVVDGTLTTPILLRGRDVGAGLVVHSLSKSIGGHGN
                     ALGGAVVDTGLFDWAAYANILPAYRKGASEKWGLLQVRKKGLRDMGGTLRADDAHRIA
                     TGAETLELRMRRVCDNALALARWLERQPAVARVHYPGLESHPQHARAQALFAGGFGPL
                     MSFELRDDVDCFAFLDALRLVIVSSHLADNRTLAIPVAHTIYYEMGPARRAEMGISDG
                     MIRLSVGIEAIDDLIDDFDRALKAVAG"
     gene            complement(2517..3419)
                     /locus_tag="VF453_17535"
     CDS             complement(2517..3419)
                     /locus_tag="VF453_17535"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7689514.1"
                     /translation="MSDALPELQILVARHARAGASATSLAGVRLRSSPVPTEATHCMR
                     EPGVGLVVQGAKRTVVGDRVFEYGAGDFVVTSVDLPIATRITHASPGRPYLACRLALR
                     PAAIASLLLDAPDDGALAAVPCGLGVHVASRELLDAFVRLLRLLGAPRDAPVLQPLIE
                     REILWRLLQGPQGGRVRQIGLADSRLARVSRAIRHIREHYAEAIAIEELARLAAMSAS
                     AFHRHFRLVTAMTPIQFQKQIRLQEARSRLLAEAGDAASVGHAVGYDSPSQFSREYSR
                     MFGAPPGRDVARLRLGASGERSAA"
BASE COUNT          490 a         1369 c         1272 g          448 t
ORIGIN      
        1 gcccgcctgc gcgcccgcgc ggcgcgcggc gcgtggccgt cctcgccggc cgcgcccgac
       61 gacgggccgc ccgagctgat cagcgactac ggcgcgaccg acccggccga attcttcgcc
      121 gtcgtgaccg aagtcttcta cgagcagccc gccgccctcg ccgtgcgcca tcccgcgctg
      181 ttcgaggtgc tgcgcggcta ctaccgcgtc gacccgcgcg aatggcactg aggggtcgcc
      241 gcggccgtcg agcggcggcc gcgcaacgct gcgttcgaat cgtttgagcc ctcgccgggc
      301 cccgcgggcg cagacttctc ctcacggaca cgacgtccat cgccaaccac caggagagac
      361 ccgatgctca cgctccgcaa ggccagcgac cgcggccacg ccgaccacgg ctggctcagc
      421 agccaccaca gcttctcgtt cgccgactac cacgacccgg cgcacatggg cttcggcaac
      481 ctgcgcgtcc tcaacgacga cacggtcgcg ccgggcaccg gcttcggcac gcacggccac
      541 cgcgacatgg agatcgtcag ctacgtcctc gacggcgcgc tcgcgcatgc cgacagcatg
      601 ggcaacggcc agccgggcgg cgcccacgcc ggcgtgatcg tcccgggcga cgtccagcgc
      661 atgaccgccg gcaccggcgt gcgccacagc gagttcaacc actcgaagac gcagccgacg
      721 catttcctgc agatctggct gctgcccgag cgcctcggcc tcgcccccgg ctacgagcag
      781 gtgaacgtgc cggcggcgtc caagcgcggg cggctcgcgc tgatcgccgc gccgcaggcc
      841 gcgcccggcg cggtgcgcct gaacgcggac gcgcgcatcc tcgcgggcct gttcgacggc
      901 gacgaacgcc agtcgctgcc gctgcccgcg ggtcgattgg cgtacgtgca cgtcgcccgc
      961 ggggctctgc gcgtcaacgg gcaggcgctc gaggccggcg acgcgctgaa ggtgcgcgac
     1021 gaggccgagc tcgtgctcga ggacggtcgc ggcgccgagg tgctggtgtt cgacctcgcg
     1081 ccctgacgcc ggcgcggccc cgcgcgcgtg accagtgcgc agggccgcgg attcgcggcg
     1141 ggcgaagccc gtcttgcgat cccgaccgct ggcacggtgc cggcgcactg gacgagaatc
     1201 gccctttggc ctttcgccgt cccacgccga cccacgccgc catgtccgac accgccccgc
     1261 gccgccacgc ccccaccacg cgcctgctgc acgccgaccg cctcgccggc gtcgaggccg
     1321 gcgccaccca caagccgctg cacgtctcgg ccgcattcaa ctacccgagc gcgcgcgacc
     1381 tgtgcgaggt cttccagggc aaccagcccg gccacgtcta cgcgcgccag ggcaatccga
     1441 cggtgcaggc gctcgaggcc aaggtcgcga tgctcgagga cgccaagggc tgcgccgcct
     1501 tcgccaccgg catggcggcg gtcgcggccg tcttcctcgc gctgctgcgc tcgggcgacc
     1561 atgtcgtgtg cacgcgcttc ctgttcggca acaccaacag cctgatgcag acgctgcagg
     1621 ggctgggcgt cgcggtgact ttcgtcgacg cgaccgatgc cgccaacgtg cgcgccgcgc
     1681 tgacgccggc cacgcgcctg gtgttcgtcg agacgatcgc caacccggtc acccaggtcg
     1741 ccgacctggc cgcggtcggc gacgcctgcc gcgaggcggg tgtcctgtac gtcgtcgacg
     1801 gcacgctcac cacgccgatc ctgctgcggg gccgcgacgt cggcgccggg ctggtcgtgc
     1861 actcgctgag caagagcatc ggcggccacg gcaacgcgct cggcggcgcg gtggtcgaca
     1921 ccggtctttt cgactgggcg gcgtacgcga acatcctgcc cgcgtacagg aagggcgcgt
     1981 ccgagaagtg gggcctgctg caggtgcgca agaagggcct gcgcgacatg ggcggcacgc
     2041 tgcgcgccga cgacgcccac cgcatcgcca ccggcgcgga gacgctggag ctgcgcatgc
     2101 gccgcgtctg cgacaacgcg ctcgcgctgg cgcgctggct cgagcgccag cccgccgtcg
     2161 cgcgcgtgca ctaccccggc ctggagagcc atccgcagca tgcgcgcgcg caggcgctgt
     2221 tcgcaggcgg cttcggcccg ctcatgagct tcgagctgcg tgacgacgtg gactgcttcg
     2281 ccttcctcga tgcgctgcgg ctggtgatcg tctccagcca cctcgcggac aaccgcaccc
     2341 tcgcgattcc cgtcgcgcac acgatctact acgagatggg gcccgcacgc cgcgccgaga
     2401 tgggcatctc ggacggcatg atccggttgt cggtgggcat cgaggcgatc gacgacctca
     2461 tcgacgattt cgatcgggcg ctgaaggcgg tcgcgggctg atccgcccgc cgaacctcag
     2521 gcggcgctgc gctcgccgga cgcccccagc cgcaggcgcg ccacgtcgcg ccccggcggc
     2581 gcgccgaaca tccggctgta ctcgcggctg aactgcgacg ggctgtcgta gccgaccgcg
     2641 tggccgaccg aggccgcgtc gccggcctcg gccagcaggc gcgatcgcgc ctcctgcagg
     2701 cggatctgct tctggaactg gatcggcgtc atcgccgtca ccaggcggaa gtggcgatgg
     2761 aacgccgacg cgctcatcgc cgccagccgc gcgagctcct cgatggcgat cgcctcggcg
     2821 tagtgctcgc ggatgtggcg gatcgcgcga ctcacgcggg cgaggcggct gtcggccagg
     2881 ccgatctggc gcacgcggcc gccctgcggg ccttgcagca gccgccacag gatctcgcgc
     2941 tcgatcagcg gttgcaggac gggcgcgtcg cgcggagcgc ccagcagccg cagcaggcgc
     3001 acgaacgcgt ccagcagctc gcgcgacgcg acgtgcacgc ccaggccgca cggaacggcc
     3061 gcgagcgcgc cgtcgtccgg ggcgtcgagc agcagcgacg cgatcgcggc gggtcgcagc
     3121 gcgaggcggc aggcgagata aggtcggccg gggctcgcgt gcgtgatgcg ggtggcgatc
     3181 ggcaggtcga ccgatgtgac gacgaagtcg cccgcaccgt actcgaacac gcggtcgccg
     3241 accaccgtgc gcttggcgcc ctgcacgacg aggcccacgc ccggctcgcg catgcagtgc
     3301 gtcgcctcgg tgggcacggg cgaactgcgc aggcgcacgc ccgccaggga ggtcgcggac
     3361 gcccccgcgc gcgcgtggcg ggcgaccagg atctgcagtt cgggcagcgc gtcggacatg
     3421 cgcgcattgg agcgcgccgc ctgcgccgcc gcaagcgtgc cccgcaggcc ggccgcggga
     3481 tcggcaggat cgtgcaatcg gcggcgaatc ccatgctacg gcgcccggcc gcgcgcgacg
     3541 gacgatggcg gccatcgcaa cgacgggaat cgccccatg
//
LOCUS       DASVLN010000739         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_37334, whole genome shotgun sequence.
ACCESSION   DASVLN010000739 DASVLN010000000
VERSION     DASVLN010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37334"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VF453_17540"
     CDS             <1..786
                     /locus_tag="VF453_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HEX7689515.1"
                     /translation="ETARGPIEARSVVVATGGLPVPKIGATDFGLRLARQFGHAIVEP
                     RPALVPLTFDAAEWAPFAALAGVSLEVGIAAGSGKGRAHFVEDLLFTHRGLSGPAVLQ
                     ASSYWTPGEALEIALAPPLDWARTLAQAKAASRRQLGNELGQHLPQRLAQAWIARLGL
                     PAERPLPELGDKALRQLAESLSSWRVRPDGTEGWRKAEVMAGGVDTREIDSRTLESRR
                     VPGLHFIGEVLDVTGWLGGYNFQWAWASAHACAEGLAQRPNSG"
     gene            931..1143
                     /gene="rpsU"
                     /locus_tag="VF453_17545"
     CDS             931..1143
                     /gene="rpsU"
                     /locus_tag="VF453_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX7689516.1"
                     /translation="MTTIRVKENEPFDVALRRFKRTIEKLGLLTELRAREFYEKPTAE
                     RKRKKAAAVKRHYKRVRSMQLPKKLY"
     gene            1325..>1833
                     /locus_tag="VF453_17550"
     CDS             1325..>1833
                     /locus_tag="VF453_17550"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX7689517.1"
                     /translation="MTSALLFGATGLVGGLVLRALLADPAWERVTIVVRRPQPAPDPR
                     VTVVVADLDTLESVRTQLAGEAVFLAIGTTRAKTPDLAEYARIDRDYPVLAARLARAN
                     GATWAGLVSSVGADERSSTFYLRTKGEAERGLRALGYPSIDIFRPGLLLGGRTERRPL
                     ERAFIAAAPA"
BASE COUNT          256 a          665 c          654 g          258 t
ORIGIN      
        1 gagaccgccc gcgggccgat cgaggcgcgc agcgtggtcg tcgcgaccgg cggcctgccg
       61 gtgcccaaga tcggcgcgac cgacttcggc ctgcggctcg ccaggcagtt cggacacgcg
      121 atcgtcgaac cgcgcccggc gctggtgccg ctgaccttcg acgccgcgga gtgggcgccg
      181 ttcgcggcgc tggcgggagt gtcgctggag gtcggcatcg cggccggcag cggcaagggc
      241 cgggcgcatt tcgtcgagga cctgctgttc acgcaccgcg gcctgagcgg cccggccgtg
      301 ctgcaggcgt cgagctactg gacgcccggc gaagcgctgg agatcgcgct cgcgccgccg
      361 ctcgactggg cgcgcacgct cgcgcaggcc aaggcggcgt cgcggcgcca gctcggcaac
      421 gagctcggcc agcacctgcc gcagcggctc gcgcaggcct ggatcgcacg cctcgggctg
      481 ccggccgagc gcccgctgcc cgaactcggc gacaaggcgc tgcgccagct ggcggagtcg
      541 ctgtcgagct ggcgcgtgcg tcccgacggc accgagggct ggaggaaggc cgaggtcatg
      601 gccggcggcg tggacacgcg cgagatcgac tcgcgcaccc tcgagagccg ccgcgtgccg
      661 gggctgcatt tcatcggcga ggtcctggac gtcaccggct ggctgggcgg ctacaacttc
      721 cagtgggcat gggccagcgc gcatgcctgt gccgaagggc tggcacaacg cccgaattcg
      781 ggctagaatc ccctgctttg ctggagctcg acccaaaatc gaggccagaa gtcgcgtgtc
      841 gatctttgtc cgacttccgg aagccgtgcc ccgcgcacgg gtctggacaa ggactccgag
      901 cgcaatcacc cccgaaggat ccttcgctac atgaccacca tccgagtcaa ggaaaacgag
      961 ccgttcgacg tggccctgcg ccgcttcaag cgcaccatcg agaagctggg tctgctgacc
     1021 gaactgcgcg cccgcgagtt ctacgagaag ccgaccgccg aacgcaagcg caagaaggcc
     1081 gccgccgtga agcgccacta caagcgcgtt cgcagcatgc agctgccgaa gaagctctac
     1141 tgagcggctt cgaactcgtt ttccgccggg acggctcgtc cgtccggtcg aacacaagcc
     1201 cgcacgggac gccgtggcgg gcttttgccc tttctggcgc cgagctttcc ggtgccggcc
     1261 cttgccacgc aagtcgcctc caacggacgt gcgcgatcgc cgcgtttggc gcgatgatgg
     1321 cgggatgacc tccgccctgc tcttcggcgc gaccggcctc gtcggcggcc tggtgctgcg
     1381 cgcgctgctg gccgatcccg cctgggagcg cgtgacgatc gtcgtgcggc ggccgcagcc
     1441 ggcgcccgat ccacgcgtca ccgtggtcgt cgccgacctg gacacgctgg aatccgtgcg
     1501 cacgcagctg gccggcgagg cggtgttcct cgcgatcggc acgacgcgcg cgaagacgcc
     1561 cgacctcgcc gaatacgcgc gcatcgatcg cgactacccc gtgcttgccg cccgcctcgc
     1621 gcgcgcgaac ggcgcgacct gggcgggcct ggtctcgtcg gtcggtgcgg acgagcgctc
     1681 gtcgaccttc tacctgcgca ccaagggcga ggcggagcgg gggctgcggg cgctcggcta
     1741 cccgtcgatc gacatcttcc gccccggcct gctgctgggc gggcgcaccg agcgccgacc
     1801 gctggagcgc gcgttcatcg ccgccgcgcc ggc
//
LOCUS       DASVLN010000740         1937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_92225, whole genome shotgun sequence.
ACCESSION   DASVLN010000740 DASVLN010000000
VERSION     DASVLN010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92225"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1937)
                     /locus_tag="VF453_17555"
     CDS             complement(<1..>1937)
                     /locus_tag="VF453_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HEX7689518.1"
                     /translation="PAALAAAFAAAALAGCGASHQPELSQKPSFVGDVTKTTYDGSTD
                     DLLTGGLGRVGLLSANVPQVSTPPKANELRRLAIYTNYRALVDTTQAGGYGVLYGPNV
                     DIHGVAGSGDGRIPGVEYRAYDDDGTGQRNVTMVTQVPGSFDASAPCLLAVASPGSRG
                     VYGGISVAEWGLKHGCAVVLVDKGTGAAPHDLHDDTVALVDGTRESAASAATAAAFNA
                     GLSTTERASFDASYPNRFAFKHAHSGRNPEKDWGRFVLDGVKFGFWAMNQAMGPVTFN
                     GEHEDRFKPDNTLVIAAGASNAGGAALAAAEQDTEGLIDGVAVSEPGVEMPANPGVTV
                     QRGSTTIATVARPLYDFTSYANLYQACASQAAALASSPAVSYVAAANAAARCNSLHAN
                     GLLSSTTTAAQADEALQKLHDYGWEAESDDLHASLAAFQVAPAVTVTFANALARAKVS
                     DDLCGFSFGAVTGAGAPTALDAATLGSMYGTGNGIPPTAGVQIINDRNPGGPMADPLS
                     SSPSSGAADLNLDGALCLRSLLTGSDAPSQALQKGLGEIRVTGRLNGKPVVIVHGRSD
                     ALLPVNHTSRPYAALAAGAGDGKLSYIEVTNAQHFDAFIDAVPGYDTRYVPLHLYLAR
                     ALDAVYANLKSGTALPPSQVVR"
BASE COUNT          244 a          684 c          728 g          281 t
ORIGIN      
        1 cgcaccacct ggctgggcgg cagcgccgtg ccgctcttca ggttcgcgta gacggcgtcg
       61 agcgcgcgcg ccaggtacag gtgcagcggc acgtagcgcg tgtcgtagcc cggcacggcg
      121 tcgatgaacg cgtcgaagtg ctgcgcgttg gtcacctcga tgtacgagag cttgccgtcg
      181 cccgcgccgg cggcgagcgc ggcatacgga cgggacgtgt ggttgaccgg cagcagggca
      241 tccgagcgcc cgtgcacgat cacgaccggc ttgccgttca ggcggccggt gacgcggatc
      301 tcgcccaggc ccttctgcag cgcctgcgag ggcgcgtcgc tgccggtcag cagcgaccgc
      361 aggcacaggg ccccgtccag gttcaggtcg gccgcgccgg acgacggcga cgacgaaagc
      421 ggatcggcca tcggcccgcc gggattgcga tcgttgatga tctgcacgcc ggcggtcggc
      481 gggatgccgt tgccggtgcc gtacatcgag ccgagcgtcg ccgcgtcgag cgccgtcggc
      541 gcgcccgcgc ccgtcaccgc gccgaagctg aagccgcaga ggtcgtcgct gaccttcgcg
      601 cgcgcgagcg cgttggcgaa ggtcaccgtc accgccggag cgacctggaa tgccgcgagc
      661 gaggcgtgca ggtcgtcgga ctcggcctcc cagccgtagt cgtgcagctt ctgcagcgcc
      721 tcgtcggcct gggccgcggt cgtcgtcgac gagagcaggc cgttggcatg cagcgagttg
      781 cagcgcgccg cggcgttggc cgccgcgacg tacgagaccg ccggcgagct cgcgagcgcc
      841 gccgcctgcg acgcgcaggc ctggtagagg ttcgcgtagc tcgtgaagtc gtagagcggc
      901 cgggcgaccg tcgcgatcgt cgtgctgccg cgctgcaccg tcacgccggg attggcgggc
      961 atctcgacgc ccggctcgga gacggcgacg ccgtcgatca ggccttcggt gtcctgctcc
     1021 gccgccgcga gcgccgcgcc gccggcgttg gacgcacccg ccgcgatcac gagcgtgttg
     1081 tccggcttga agcgatcctc gtgctcgccg ttgaacgtga ccggccccat cgcctggttc
     1141 atcgcccaga acccgaactt gacgccgtcc agcacgaagc ggccccagtc cttctcgggg
     1201 ttgcggccgg agtgcgcgtg cttgaacgcg aagcggttcg ggtagctggc gtcgaagctc
     1261 gcccgctcgg tcgtgctcag ccccgcgttg aaggcggcgg ccgtggcggc gctggccgcg
     1321 ctctcgcgcg tgccgtcgac gagggcgacg gtgtcgtcat gcaggtcgtg cggcgcggcg
     1381 ccggtgccct tgtcgaccag cacgaccgcg cagccgtgct tcaggcccca ctcggccacc
     1441 gagatcccgc cgtagacacc gcgcgagccg ggcgacgcga ccgccagcag gcagggagcc
     1501 gaggcatcga agctgcccgg cacctgggtc accatcgtga cgttgcgctg gccggtgccg
     1561 tcgtcgtcgt aggcacggta ttcgacgccg gggatgcggc catcgccgct gccggcgacg
     1621 ccgtggatgt cgacgttcgg gccgtacagc accccgtatc cgccggcctg cgtcgtgtcg
     1681 accagcgcgc ggtagttggt gtagatcgcg agccgccgca gctcgttcgc cttgggcggc
     1741 gtggagacct gcggcacgtt ggcgctcagc aggccgacgc gcccgaggcc gccggtgagc
     1801 aggtcgtcgg tcgagccgtc gtaggtcgtc ttcgtgacgt cgccgacgaa cgagggcttc
     1861 tggctcagct cgggctggtg gctggcgccg cagccggcca gcgccgccgc ggcgaacgcg
     1921 gccgccagcg cggcggg
//
LOCUS       DASVLN010000741         7797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_523228, whole genome shotgun sequence.
ACCESSION   DASVLN010000741 DASVLN010000000
VERSION     DASVLN010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523228"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VF453_17560"
     CDS             complement(<1..201)
                     /locus_tag="VF453_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689519.1"
                     /translation="MRTHPVLAVLVVAATIGTVTVFGQQPVPSAPAASRPAHAPRHAD
                     PKLDAASAVPAAAAWLVPPSAPA"
     gene            complement(212..1969)
                     /locus_tag="VF453_17565"
     CDS             complement(212..1969)
                     /locus_tag="VF453_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX7689520.1"
                     /translation="MKKLPSVDVLVVGFGWTGAIVAQELTEAGLDVLALERGAWADTS
                     TEFATTFAQDELRYFWRHALFQAPGHDTQTVRNDPGQVALPMRRLGSFLPGTNVGGAG
                     VHWNGQTWRFLPSDFVARSHNLQRYGAAAIPDTMTIQDWGVTYDELEPHYDRFERLCG
                     ISGKAGNLQGRIQDGGNPFEGPRSREYPTPPMKMSYPPTLFAEAARGLGLHPFPQPSA
                     NLSQPYVNPLGVRLQACTYCGFCEKFACGNYSKASPQTTILPVLMPKPNFRLRTECEV
                     MKVNLDPSGKRATGVTYVDAAGEEFEQPAELVILCSYILSNVRLMLLSGIGKPYDPAT
                     GQGVVGKNYAYQITSSVNLFFDDKILNPFIGAGAHGMLVDEWNGDNFDHTGLGFIGGG
                     YVGVVQTGARPIETHPVPEGTPAWGSAFKKAIAANYLRTMSIATHGAVMSHRGNYLDL
                     DPTYRDVYGRPLLRMTFDYTDNEHRMSDYLTDRAAEIARAMKPREIKVNKRKGSWDCV
                     PYQTTHNTGGTIMGADPASSVVNKYLQSWDVPNVFVMGAGAFPQNPGYNPTGTVAALA
                     YASAQAIVERYVKAPGALA"
     gene            complement(1966..2718)
                     /locus_tag="VF453_17570"
     CDS             complement(1966..2718)
                     /locus_tag="VF453_17570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEX7689521.1"
                     /translation="MDDKRLSRRGFLTAGAAIGGLPAASATAALLGGDDPPWQEGRAN
                     APRAVDPDAASWAFFTAAEGAFVTAATSRLIPADELGPGAVEAGVPLFIDRQLAGAYG
                     RAERWYMQGPWAPGLPTQGYQTRLTPAGLYRAAIKAVDAAVGREQSGTRFAALAPDAQ
                     DDVLRRLEKGELALDGVDAKAFFAQLLQNTLEGFWSDPIYGGNRGMAGWKLIGFPGAR
                     YDQSPYVQQHGQPYPLPPVGLSGRVEWIRSAS"
     gene            complement(2957..4351)
                     /gene="fumC"
                     /locus_tag="VF453_17575"
     CDS             complement(2957..4351)
                     /gene="fumC"
                     /locus_tag="VF453_17575"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX7689522.1"
                     /translation="MSIEPRLEHDAWGDIPVPGEHLWGAQTQRSIEHFAISTERMPPE
                     LLHALALVKSAAARVNHRAGRLKADKAEAIERAAAEVASGRLDAEFPLSVWQTGSGTQ
                     SNMNMNEVLANRASQLLGGPLGLKRVVHPNDEVNLGQSSNDVFPTAMHVAAVRGIATR
                     VIPAVQALRQTLQAKAAAFADIVKIGRTHLQDATPLTLGQEISGWVEQLAQSERQLEA
                     VLPDLRELALGGTAVGTGLNTPASFGAEVAAELDRVTGLGFVSAPNKFAALAAHDAIV
                     RAHGALKALAAAMTKIANDIRWLASGPRSGLGEIAIPENEPGSSIMPGKVNPTQCEAM
                     TMLCAQVMGNDVAVTIAGASGNFELNVFKPVMIHNLLQSVRLLADGLHGFDRHCAQGI
                     EPRRDRIAELLKNSLMLVTALNPHIGYDAAAQIARTAHAQGLTLRDAAIASGKLTGEQ
                     FDAWVRPEDMIRPD"
     gene            4500..5042
                     /gene="phaR"
                     /locus_tag="VF453_17580"
     CDS             4500..5042
                     /gene="phaR"
                     /locus_tag="VF453_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX7689523.1"
                     /translation="MVTTRRGTKNADEGQGPSGPRILKKYPNRRLYDTLTSSYITLAD
                     VKKMVLDGESFEVRDAKTGDEITRSILLQIILEEETGGVPMFSTPMLANIIRFYGHAM
                     QGVMGTWLEKNLQALAEMQAGLAQGGPTVPGFNPFAQNLMGSYFEQSKKMFEQMQEQM
                     SKGEGLFPMPPIPGFTPPKR"
     gene            complement(5091..5492)
                     /locus_tag="VF453_17585"
     CDS             complement(5091..5492)
                     /locus_tag="VF453_17585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7689524.1"
                     /translation="MRISARVRNAGHRHDVELRTGDQATTLAVSAKAAGGGSAVNGGE
                     FLMLALATCYCNDLYREAARLGLTLDAVEVEAHASFDGIGLAATDIRYRATVTSGTAT
                     PAQIEDLVRQTDAVAEVHNTLRAGVPVTLSR"
     gene            complement(5489..5788)
                     /locus_tag="VF453_17590"
     CDS             complement(5489..5788)
                     /locus_tag="VF453_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX7689525.1"
                     /translation="MCRNIRTLFNFEPPATEVEIRDAALQFVRKLSGFTVPSKANEAA
                     FEQAVADIAATTRTLIASLVTTAPPHDREVEAAKARARSAERFGTRAQAGAGAKP"
     gene            5917..7317
                     /gene="rimO"
                     /locus_tag="VF453_17595"
     CDS             5917..7317
                     /gene="rimO"
                     /locus_tag="VF453_17595"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX7689526.1"
                     /translation="MESTTYSTESPSAAGREPTIAFVSLGCPKALTDSELILTQLRAE
                     GYGTSKTYAGADLVVVNTCGFIDDAVKESLDTIGEALAENGKVIVTGCLGAKAGHASD
                     NLVREIHPKVLAVTGPHATQEVMDAVHRHVPKPHDPFVDLVPPQGIKLTPRHYAYLKI
                     SEGCNHRCTFCIIPSMRGDLDSRAVGDVLSEAQRLFEAGVKELLVVSQDTSAYGVDVQ
                     YRTGFWDGRHVKTRMLDLCERLGELAARHGAWVRLHYVYPYPHVDQVIPLMAEGRILP
                     YLDVPFQHAHPDVLKRMKRPASGERNLERIQRWREMCPELVIRSTFIAGFPGETDDEF
                     ETLLDFLREAQIDRAGCFAYSPVEGATANALEGQLPQAVREERRARFMALAEGVSTAR
                     LQRRVGATMQVLVDKAPAMGRKGGVGRSYADAPEIDGVVRLLPPEKASKTLKVGEFTR
                     ARIVATEGHDLVGLPI"
     gene            7329..>7797
                     /locus_tag="VF453_17600"
     CDS             7329..>7797
                     /locus_tag="VF453_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX7689527.1"
                     /translation="MPDPSPPGGDRDGAPSTALIHHSYAPPAGFGAIPPGVFKASTVL
                     FDSVAALRSHQWLEKRSYSYGLHGTPTSFTLEARLATLEGGTHALLAPSGLAAVALVN
                     QALLSAGDAVLLPDNVYFPNRNLAAHELARWGIAHRLYDPLDPASLDAALGPDV"
BASE COUNT         1095 a         2729 c         2818 g         1155 t
ORIGIN      
        1 ggccggcgcc gagggcggca ccagccatgc ggccgcggcg ggcacggcgg acgcggcgtc
       61 gagcttcggg tcggcgtggc gcggcgcgtg tgctggcctg gacgcggccg gtgcgctcgg
      121 cacgggctgc tgcccgaaca cggtcacggt gccgatggtc gctgccacga cgaggacggc
      181 caggacggga tgggtgcgca tggcggcgtc ctcaggcgag cgcaccgggc gccttgacgt
      241 agcgctccac gatcgcctgc gccgacgcgt aggccagcgc cgcgacggtg ccggtcgggt
      301 tgtagccggg gttctgcggg aacgcgccgg cgcccatcac gaacacgttg ggcacgtccc
      361 acgactgcag gtacttgttg acgacgctgc tcgcgggatc cgctcccatg atcgtgccgc
      421 cggtgttgtg cgtggtctgg tagggcacgc agtcccagct tcccttgcgc ttgttgacct
      481 tgatctcgcg cggcttcatc gcgcgcgcga tctccgcggc gcgatcggtg aggtagtccg
      541 acatccggtg ctcgttgtcg gtgtagtcga aggtcatgcg cagcagcggg cggccgtaga
      601 cgtcgcggta ggtcgggtcg aggtccaggt agttgccgcg atggctcatc accgcgccgt
      661 gcgtggcgat gctcatcgtg cgcaggtagt tcgccgcgat ggccttcttg aaggccgagc
      721 cccaggccgg cgtgccctcg ggcaccggat gcgtctcgat cggccgggcg ccggtctgca
      781 cgacgcccac gtagccgccg ccgatgaagc cgaggccggt gtggtcgaag ttgtcgccgt
      841 tccactcgtc gaccagcatg ccgtgcgcgc ccgcgccgat gaacgggttg aggatcttgt
      901 cgtcgaagaa caggttgacc gaggacgtga tctggtacgc gtaattcttg ccgaccacgc
      961 cctgcccggt ggccgggtcg tacggcttgc cgatgcccga gagcagcatc agccgcacgt
     1021 tgctcaggat gtaggagcac aggatgacga gctcggcggg ttgctcgaat tcctcgcccg
     1081 ccgcgtcgac gtaggtcacg cccgtcgccc gcttgccgct cgggtcgagg ttgaccttca
     1141 tcacctcgca ttcggtgcgc aggcggaagt tgggcttggg catgagcacc ggcaggatgg
     1201 tcgtctgcgg gctcgccttc gagtagttgc cgcacgcgaa cttctcgcag aagccgcaat
     1261 acgtgcaggc ctgcaggcgc acgcccagcg ggttcacgta gggctgcgac aggttcgccg
     1321 agggctgcgg gaacggatgc aggccgagcc cgcgcgcggc ctccgcgaac agggtcggcg
     1381 gatacgacat cttcatcggc ggcgtcgggt actcgcgcga gcgcgggccc tcgaacgggt
     1441 tgccgccgtc ctggatcctg ccctggaggt tgccggcctt gccgctgatg ccgcacaggc
     1501 gctcgaagcg gtcgtagtgg ggctcgagct cgtcgtaggt gacgccccag tcctggatcg
     1561 tcatcgtgtc ggggatcgcg gcggcgccgt agcgctgcag gttgtgcgag cgcgcgacga
     1621 agtccgacgg caggaagcgc caggtctggc cgttccagtg cacgccggcg ccgccgacgt
     1681 tggtgccggg caggaacgag ccgaggcggc gcatcggcag cgcgacctgg ccggggtcgt
     1741 tgcgcacggt ctgcgtgtcg tggcccggcg cctggaacag ggcgtggcgc cagaagtagc
     1801 gcagctcgtc ctgcgcgaag gtggtcgcga actcggtcga cgtgtcggcc caggcgccgc
     1861 gctccagcgc gagcacgtcg aggccggcct cggtgagctc ctgcgcgacg atggcgccgg
     1921 tccagccgaa gccgacgacg agcacgtcca cggacggcag cttcttcatg acgcgctcct
     1981 gatccattcg acgcgccccg acagtcccac cggcggcagc gggtagggct ggccgtgctg
     2041 ctgcacgtag ggcgactggt catagcgcgc gcccgggaag ccgatcagct tccagcccgc
     2101 catgccgcgg ttgccgccgt agatcgggtc gctccagaag ccctccagcg tgttctgcag
     2161 caactgcgcg aagaaggcct tggcgtcgac gccgtcgagt gcgagctcgc ccttctcgag
     2221 gcgacgcagc acgtcgtcct gcgcgtcggg agcgagcgcg gcgaagcgcg tgccggactg
     2281 ctcgcggccg accgcggcgt cgaccgcctt gatcgccgcg cgatagaggc ccgcgggcgt
     2341 gagccgcgtc tggtagccct gcgtgggaag gcccggcgcc cacgggccct gcatgtacca
     2401 gcgctcggcg cggccgtagg cgccggcgag ctggcggtcg atgaacagcg gcacgccggc
     2461 ctccaccgcg cccgggccga gctcgtcggc cgggatcagg cgactcgtcg ccgcggtgac
     2521 gaacgcccct tcggccgccg tgaagaaggc ccacgaggcc gcgtccgggt cgacggcccg
     2581 tggcgcgttg gcgcggccct cctgccaggg cgggtcgtcg ccgccgagca gtgccgcggt
     2641 ggcggaggcc gcgggcagcc cgccgatcgc cgcgcccgcg gtcaggaagc cgcgtcggct
     2701 caggcgcttg tcgtccatcg tcgtcccctg tcgtgcaagt cggcacgtca gggcaaacga
     2761 tgtgcgagtc agggatgacc cgaagattcc agccgcccgc gagcgccccg tgcgagacca
     2821 gcagagtcac tccagccccg gcgctgccgc tcggacgccc caagcgagac acgggggagc
     2881 ggcagatccg gctttgccgg tctgcacgcg gccggccccc tcggggggca gcgagcgcca
     2941 gcgagcgtgg gggtcgtcag tccggtcgga tcatgtcctc cgggcgcacc caggcgtcga
     3001 actgctcgcc cgtgagcttg cccgaggcga tcgccgcgtc gcgcagcgtc aggccctgcg
     3061 cgtgcgcggt gcgcgcgatc tgcgccgcgg cgtcgtagcc gatgtgcgga ttcagcgcgg
     3121 tcaccagcat cagcgagttc ttcaggagct ccgcgatgcg gtcgcggcgc ggctcgatgc
     3181 cctgcgcgca gtgcctgtcg aagccgtgca gcccgtcggc cagcaggcgc acgctctgca
     3241 gcaggttgtg gatcatgacc ggcttgaaca cgttgagctc gaagttgccg cttgcgccgg
     3301 cgatcgtcac cgcgacgtcg ttgcccatca cctgcgcgca cagcatcgtc atcgcctcgc
     3361 actgggtcgg gttgaccttg cccggcatga tggagctgcc cggctcgttc tcggggatgg
     3421 cgatctcgcc caggccgctg cgcgggccgc tcgcgagcca gcggatgtcg ttggcgatct
     3481 tggtcatcgc cgcggcgagc gccttgagcg cgccgtgcgc gcgcacgatc gcgtcgtgcg
     3541 ccgcgagcgc cgcgaacttg ttgggggcgc tgacgaagcc caggcccgtc acgcgatcga
     3601 gttccgcggc gacctcggcg ccgaacgacg ccggcgtgtt caggccggta cccaccgccg
     3661 taccgccgag cgcgagttcg cgcaggtcgg gcagcacggc ctcgagctgg cgctcggact
     3721 gcgcgagctg ctcgacccag cccgagatct cctggcccag cgtcagcggc gtcgcgtcct
     3781 gcaggtgcgt gcggccgatc ttgacgatgt ccgcgaacgc ggcggccttg gcctgcagcg
     3841 tctggcgcag cgcctgcacc gccgggatca cgcgcgtggc gatgccgcgc accgcggcca
     3901 cgtgcatcgc ggtcgggaag acgtcgttgg acgactggcc gaggttgacc tcgtcgttcg
     3961 gatgcacgac gcgcttcagg cccagcgggc cgcccagcag ctgcgacgcg cggttggcca
     4021 gcacctcgtt catgttcatg ttgctctgcg tgcccgagcc cgtctgccac accgacagcg
     4081 ggaactcggc gtccagcctg cccgacgcca cctcggcggc cgcgcgctcg atggcctcgg
     4141 ccttgtcggc cttcagccgt cccgcgcgat ggttgacgcg cgcggccgcg ctcttgacca
     4201 gcgcgagggc gtgcagcagc tcggggggca tgcgctccgt ggagatggcg aagtgctcga
     4261 tggagcgctg ggtctgcgcg ccccagagat gctcgccggg gacggggatg tcgccccagg
     4321 cgtcgtgttc gaggcgaggt tcgatgctca tgcgcggact ccgtgcgaag acgttcgagg
     4381 gtcgggggct cggcggccgg cacggggcaa tcccttgctg cagcgcagcg gccggcctct
     4441 agaatcctcc cagtgtcgca ccgggtgcga cgtgcagccg aacgaccccg aggaactcca
     4501 tggtgaccac gcgccgcggt acgaagaacg ccgacgaagg gcagggcccg agcggcccgc
     4561 gcatcctgaa gaaatacccc aaccgccgcc tctacgacac gctcacgagc tcgtacatca
     4621 cgctggcgga cgtcaagaag atggtgctgg acggcgagtc cttcgaggtt cgcgacgcca
     4681 agaccggcga cgagatcacg cgcagcatcc tgctgcagat catcctcgag gaggagaccg
     4741 gcggcgtgcc gatgttctcg acgcccatgc tggccaacat catccgcttc tacgggcacg
     4801 ccatgcaggg cgtgatgggc acctggctcg agaagaacct gcaggccctg gccgagatgc
     4861 aggccggcct cgcccagggc ggcccgacgg tgcccggctt caatcccttc gcgcagaacc
     4921 tcatgggcag ctacttcgag cagtccaaga agatgttcga gcagatgcag gagcagatga
     4981 gcaagggcga gggcctgttc ccgatgccgc cgatcccggg cttcacgccg cccaagcgct
     5041 gaccacccgc gtcgcgcggc cggcccggcc cgcgacgcgt cggcgcggcc tcagcgcgac
     5101 agggtcaccg gcacgcccgc gcgcagcgtg ttgtggactt cggccaccgc gtccgtctgc
     5161 cggacgaggt cctcgatctg cgcgggcgtg gccgtgcccg aggtgaccgt cgcgcgatag
     5221 cggatgtccg tggcggccag gccgatgccg tcgaagctcg catgcgcctc gacctcgacg
     5281 gcgtccagcg tcaggccgag ccgcgcggcc tcgcggtaga ggtcgttgca gtagcacgtc
     5341 gccagcgcga gcatcaggaa ctcgccgccg ttgacggccg agccgccgcc cgcggccttg
     5401 gcggagacgg ccagcgtcgt cgcctggtcg ccggtgcgca gttcgacgtc gtgccggtgg
     5461 ccggcgttgc gcacgcgcgc cgagatcctc atggctttgc tcccgcgccg gcttgcgccc
     5521 gcgtcccgaa gcgctcggcc gatcgcgccc gggccttcgc ggcctcgacc tcgcggtcgt
     5581 ggggcggtgc cgtggtgacg agcgaggcga tcagcgtgcg cgtggtcgcc gcgatgtccg
     5641 cgacggcctg ctcgaaggcg gcctcgttcg ccttggacgg cacggtgaac ccgctgagct
     5701 tgcgcacgaa ctggagcgcg gcgtcgcgga tctcgacctc ggtggcgggc ggctcgaaat
     5761 tgaaaagcgt cctgatgttg cggcacatgg cggcgctcgg cggagtcgga gttggcgata
     5821 tcgtacgacc gcagcgcccc cgcggcttgg tcgacgtgga tcgtggcgct acaatgacgg
     5881 gttcgccgtg gcctggttcc accgcaagaa agaccaatgg aatcaacgac ttactcgacc
     5941 gagagcccgt ccgccgccgg cagggagccg accatcgcct tcgtcagcct cggctgcccc
     6001 aaggccttga ccgactcgga gctcatcctg acccagctgc gcgccgaggg ctacggcacc
     6061 agcaagacgt atgcgggcgc cgacctcgtc gtcgtcaaca cctgcggctt catcgacgac
     6121 gcggtcaagg agagcctgga cacgatcggc gaggcgctgg ccgagaacgg caaggtcatc
     6181 gtgacgggct gcctgggcgc gaaggccggg cacgcgagcg acaacctcgt gcgcgagatc
     6241 catccgaagg tgcttgccgt gaccggcccg cacgccacgc aggaggtgat ggacgctgtg
     6301 caccggcacg tgcccaagcc gcacgacccc ttcgtcgacc tcgtgccgcc gcagggcatc
     6361 aagctcacgc cgcgccacta cgcgtacctg aagatcagcg agggctgcaa ccatcgctgc
     6421 accttctgca tcatcccgag catgcgcggc gacctcgact cgcgcgccgt cggcgacgtg
     6481 ctgtccgagg cgcagcgcct gttcgaggcg ggcgtgaagg agctgctggt cgtcagccag
     6541 gacacctcgg cctacggcgt cgacgtgcaa taccgcaccg ggttctggga cggccggcac
     6601 gtgaagacgc gcatgctgga cctgtgcgag cgcctcggcg agctcgcggc gaggcacggc
     6661 gcctgggtgc ggctgcatta cgtgtacccg tacccgcatg tcgaccaggt catcccgctg
     6721 atggccgaag gcaggatcct gccgtacctg gacgtgcctt tccagcacgc gcaccccgac
     6781 gtcctcaagc gcatgaagcg cccggcgagc ggcgagcgca acctcgagcg catccagcgc
     6841 tggcgcgaga tgtgcccgga gctcgtgatc cgcagcacct tcatcgcggg gttcccgggc
     6901 gagaccgacg acgagttcga gacactgctc gacttcctgc gcgaggcgca gatcgaccgc
     6961 gcgggctgct tcgcctactc gccggtcgag ggcgcgacgg ccaacgcgct cgagggccag
     7021 ctgccgcagg cggtgcgcga ggagcgccgc gctcgcttca tggcgctggc ggaaggcgtg
     7081 tcgaccgcgc gcctgcagcg ccgcgtcggc gcgaccatgc aggtgctggt cgacaaggcg
     7141 ccggcgatgg gccgcaaggg cggcgtcggc cgctcgtacg cggacgcgcc ggagatcgac
     7201 ggcgtggtgc gcctgctgcc gccggagaag gcgtccaaga cgctcaaggt cggcgagttc
     7261 acgcgcgccc ggatcgtcgc gaccgaaggc cacgacctcg tcggcctgcc gatctgaaga
     7321 cgacgcgcat gcctgacccg agcccgcccg gcggcgaccg cgacggcgcg ccgtccacgg
     7381 cgctgatcca tcactcgtac gcgccgcccg ccggcttcgg cgcgatcccg ccgggcgtgt
     7441 tcaaggcctc gacggtgctg ttcgacagcg tcgcggcgct gcgctcgcac cagtggctgg
     7501 agaaacggtc gtacagctat gggctgcacg gcacgccgac ctcgttcacg ctcgaggccc
     7561 ggctcgcgac gctggagggc ggcacgcatg cgctgctggc gcccagcggg ctcgccgcgg
     7621 tcgccctcgt caaccaggcg ctgctgtcgg ccggcgatgc cgtgctgctg cctgacaacg
     7681 tctacttccc gaaccgcaac ctcgccgcgc acgagctcgc gcgctggggc atcgcgcacc
     7741 ggctctacga cccgctcgat ccggcctcgc tcgacgccgc gctcgggccc gacgtgg
//
LOCUS       DASVLN010000742         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_413953, whole genome shotgun sequence.
ACCESSION   DASVLN010000742 DASVLN010000000
VERSION     DASVLN010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413953"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VF453_17605"
     CDS             complement(<1..748)
                     /locus_tag="VF453_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7689528.1"
                     /translation="MTHDPATILPPAGARRHLALAASALASAFAAAALAGCSAHSATP
                     EPVRAVRTMTVGTLSSAVTRDYAAEVHARVESRLAFRVGGKIIQRPVNLGDRVKGGQV
                     LAEIDPADLRLGQESARAALEAAEVNYRQTATDFKRYQDLRAQGFISAADLDRRESGL
                     QAAKAQYDQARAQAGVQANQAGYAALAADAPGVITAVYADVGTVTGAGTPVVSLAHDG
                     PRDVVFAVPEDQLAVFRRLEASGGTVGVTLW"
     gene            1034..1894
                     /gene="hpnC"
                     /locus_tag="VF453_17610"
     CDS             1034..1894
                     /gene="hpnC"
                     /locus_tag="VF453_17610"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX7689529.1"
                     /translation="MSVDHYENFPVASWLCPPAMRPAVRAIYWFARTADDIADEGDAP
                     ADERRAALEAYRNDLRATLAGTAPSPRWTGVFDPLARVVAHHALPAQPLLELLDAFEE
                     DTRHAGYADRAALLAYCRRSANPVGRLLLHLHGIDDPALARRSDAVCSALQLVNFWQD
                     LAVDLPRGRCYVPRSDAAAHGVDLEALARGRAAPGDAAARALVAELCAWARGLMLEGA
                     PIALAVPGRVGWELRFVVQGGLRILEKIAAMNFTSFARRPRLRAADHLLVAARALAMA
                     SRLPTRAGHA"
     gene            1956..2804
                     /gene="hpnD"
                     /locus_tag="VF453_17615"
     CDS             1956..2804
                     /gene="hpnD"
                     /locus_tag="VF453_17615"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX7689530.1"
                     /translation="MTPEQYVNEKAQQSGSSFYYAFMFLPPVRRAAMTAFYAYCREID
                     DVVDEMHDPGVAATKLGWWKREVEASWAGNPSHPAMKALMPHVHTFGVRLDHLLAVIQ
                     GCEVDLQQTRFLDYAGLQKYCQLVAGIVGEVASNIMGRTQAQTVTFAHRLGLAMQLTN
                     IIRDVGDDARRGRIYLPVSELQQFDVKASEILKRDAPWGYSERFTALMKFQAERAHGL
                     YDEALALLPEADRNNQKPALMVGNIYRRLLREVEAADFQVLHQRISLTPLRKLWIACQ
                     TQWRGR"
BASE COUNT          379 a         1088 c         1024 g          391 t
ORIGIN      
        1 cccacagcgt cacgccgacc gtgccgcccg aggcttccag gcggcggaag acggcgagct
       61 ggtcctcggg caccgcgaag acgacgtcgc gcgggccgtc gtgcgcgagg ctcacgaccg
      121 gcgtgcccgc gccggtgacc gtgccgacgt cggcatacac cgccgtgatg acgcccggtg
      181 catcggccgc gagcgccgcg tagccggcct ggttggcctg cacgccggcc tgcgcgcgcg
      241 cctggtcgta ctgcgccttg gccgcctgca ggccgctctc gcggcgatcg aggtcggcgg
      301 cactgatgaa gccctgcgca cgcaggtcct ggtagcgctt gaagtccgtc gccgtctggc
      361 ggtagttcac ctctgcggcc tcgagggccg cgcgcgcgga ctcctggccc agccgcaggt
      421 cggccgggtc gatctccgcc agcacctgcc cgcccttgac acggtcgccc aggttgaccg
      481 ggcgctggat gatcttgccg ccgacgcgga acgccaggcg cgactcgacg cgggcatgca
      541 cctcggccgc gtagtcgcgc gtgacggccg aggacagcgt ccccaccgtc atcgtgcgca
      601 ccgcgcgcac gggttcgggc gtggcggagt gggcgctgca gccggcgagg gccgcggccg
      661 cgaacgccga ggccagcgcg gaggcggcca gcgcgagatg ccggcgagcg ccggcgggcg
      721 ggaggatcgt ggcaggatca tgggtcatgg cggggtgtcc atggtcggcg ccctggtcgg
      781 acgctcgggt tgattcaatt actgaccggt cagtcagtaa tgtagacggc gcttgccatc
      841 ccgtcaatgc ggcggcgcgg gtttgtccgg agatcgccgc ggccgccgca gcggccgcgg
      901 ccgcggtcgc tgcacgaagc ctggcgcgcc ggcgcggcgc acgagtcgac gcgcccgacc
      961 cgtccgtccg atttcctcct gccacgcccg cacaggcaca atccggcgcc cgccccgcac
     1021 gcgcctgccg ccgatgagcg tcgaccacta cgaaaacttc cccgtcgcct cgtggctgtg
     1081 cccgcccgcg atgcgtccgg ccgtgcgcgc gatctactgg ttcgcgcgca ccgccgacga
     1141 catcgccgac gagggcgacg cgcccgccga cgagcgccgc gccgcgctgg aggcctatcg
     1201 caacgacctc cgcgcgaccc tggccggcac cgcgccgtcg ccgcgctgga cgggcgtgtt
     1261 cgacccgctc gcccgcgtcg tcgcgcacca cgcgctgccg gcgcagccgc tgctggaact
     1321 gctggacgcc ttcgaggagg acacgcgcca cgcgggctac gcggatcgcg ccgccctgct
     1381 cgcctactgc cgtcgctccg cgaacccggt cggccggctc ctgctccacc tgcacggcat
     1441 cgacgacccc gcgctcgcga ggcgcagcga cgccgtctgt tcggcgctcc agctggtcaa
     1501 cttctggcag gacctcgccg tcgacctgcc gcgcggccgc tgctacgtgc cgcggtcgga
     1561 tgcggcggcc cacggtgtcg acctggaggc actcgcccgc ggccgcgcgg cgccaggcga
     1621 cgccgccgcg cgcgccctcg tcgccgagct ctgcgcctgg gcacgcggac tgatgctcga
     1681 aggcgcgccc atcgcgctcg cggtgccggg acgcgtcggc tgggagctgc ggttcgtcgt
     1741 ccagggcgga ctgcgaattc ttgagaaaat cgccgccatg aacttcacga gcttcgcccg
     1801 ccgcccgcgc ctgcgcgccg ccgatcacct gctggtcgcc gcgcgcgcgc tcgccatggc
     1861 ttcccgcctc ccgacccggg cagggcacgc atgagctact gcccggctgt ccagaacggg
     1921 gctcgcgccg ccgcgccccg cacggaggtt tccacgtgac ccccgagcag tacgtcaacg
     1981 agaaggcgca acagagcgga tcctcgttct actacgcgtt catgttcctg ccgccggtgc
     2041 ggcgcgccgc catgacggcg ttctacgcct actgccgcga gatcgacgac gtcgtcgacg
     2101 agatgcacga ccccggggtc gccgcgacca agctcgggtg gtggaagcgc gaggtcgagg
     2161 cctcctgggc cggcaacccg tcgcatccgg cgatgaaggc gctgatgccg cacgtgcaca
     2221 ccttcggcgt gcggctggac cacctgctgg ccgtcatcca gggctgcgag gtcgacctgc
     2281 agcagacgcg cttcctcgac tacgcgggcc tgcagaagta ctgccagctg gtggccggca
     2341 tcgtcggcga ggtcgcgagc aacatcatgg gccgcacgca ggcgcagacg gtgaccttcg
     2401 cgcaccgcct gggcctggcg atgcagctga ccaacatcat ccgcgacgtc ggcgacgacg
     2461 cacgccgcgg tcgcatctac ctgcccgtgt cggagctgca gcagttcgac gtcaaggcga
     2521 gcgagatcct gaagcgcgat gccccctggg gctacagcga gcgcttcacg gcgctgatga
     2581 agttccaggc cgagcgggcg cacgggctgt acgacgaggc gctcgccctg ctgcccgagg
     2641 ccgaccgcaa caaccagaag cccgcgctga tggtgggcaa catctaccgc cggctgctgc
     2701 gcgaggtcga ggccgccgac ttccaggtgc tgcaccagcg catctcgctg acgccgctgc
     2761 gcaagctgtg gatcgcctgc cagacgcagt ggcgcgggcg ctgatcgccg cgcgcgatac
     2821 ccgtcgcggc gggccggcgc gggcggccgc gtcgtgagga tcgccgtcgt cggtgccggc
     2881 cg
//
LOCUS       DASVLN010000743         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_307951, whole genome shotgun sequence.
ACCESSION   DASVLN010000743 DASVLN010000000
VERSION     DASVLN010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307951"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..2090
                     /gene="tkt"
                     /locus_tag="VF453_17620"
     CDS             39..2090
                     /gene="tkt"
                     /locus_tag="VF453_17620"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7689531.1"
                     /translation="MPANIDQLCIDTLRFLSVDMVQKADSGHPGLPLDAAPMAYALWT
                     RHLKHAPKSPLWADRDRFVLSAGHGSALLYSLLQVTGYGLTIDDLKQFRQWDSRTPGH
                     PERGHTAGVEVTTGPLGQGLANAVGMAIAEAQLAATYNRDGHAIIDHRTWVIASDGDL
                     MEGVAAEAISLAGHLKLHKLVVLYDDNEVTLSAGAGITFTEDKAARLRACGWHAIRVE
                     DGNDVEAIDRALSEAQAQSGKPTLILVRTHLGFGSPKQDSYKAHGSPLGEEDVARTKQ
                     RLGWPAEPDFFVPEDAARKFGEAVERGAKLERDWQARFEAYRQAYPDLARELDDRLHG
                     RLPDGWQQALPVFPADDKGMSTRVAGKKILAAIGPKLPALVGGSADLDPSTFTPLVGA
                     GDFGPPPAADAKAIEGTDAGGISYAGRNLHFGVREHAMGAIVNGLAAHGGFVPYGATF
                     LIFSDYMRPAIRLAALQKLHALQVFTHDSIALGEDGPTHQPVEQLMSLRAIPGLTTIR
                     PADANETAVAWRVALEAKDRPVLLVFTRQDVPTLDRTRHASAEGLAKGAYVLADAEGG
                     APEIVLIASGSEVGLIVAAARTLAAEGVRARCVSMPCWDLFAQQPKEYRDAVLPPSAK
                     RRLAVEAGTTLGWERWVGDEGDVIGLDHFGASAPAKKLLAEFGFSPEHVVERARRLLR
                     R"
BASE COUNT          311 a          802 c          764 g          273 t
ORIGIN      
        1 gatctccggc gcgctccatc cgtcgtaaag gttcctccat gcccgccaac atcgaccagc
       61 tctgcatcga cacgctgcgc ttcctgtccg tcgacatggt gcagaaggcc gacagcggcc
      121 accccggcct gccgctggac gccgcgccga tggcctatgc cctgtggacg cgccacctga
      181 agcatgcgcc gaagagcccg ctgtgggccg accgcgaccg cttcgtgctc tccgccggcc
      241 acggctcggc gctgctgtac agcctgctgc aggtgacggg ctacggcctc acgatcgacg
      301 acctgaaaca gttccggcaa tgggacagcc gcacgcccgg ccaccccgag cgcggccaca
      361 ccgcgggcgt cgaggtcacg accggcccgc tcggccaggg cctggccaac gcggtgggca
      421 tggcgatcgc ggaagcccag ctcgcggcga cctacaaccg cgacggccac gcgatcatcg
      481 accaccgcac ctgggtcatc gccagcgacg gcgacctgat ggaaggcgtc gccgcggagg
      541 cgatctcgct ggccggccac ctgaagctgc acaagctcgt cgtgctgtac gacgacaacg
      601 aggtcacgct ctcggccggc gcgggcatca cgttcaccga ggacaaggcc gcccgcttgc
      661 gcgcatgcgg ctggcacgcg atacgcgtcg aggacggcaa cgacgtcgag gcgatcgacc
      721 gcgcgctgtc cgaggcgcag gcgcagtccg gcaagccgac gctgatcctc gtgcgcacgc
      781 acctgggctt cggctcgccc aagcaggaca gctacaaggc gcacggctcg ccgctgggcg
      841 aggaggacgt cgccaggacc aagcagcgcc tgggctggcc ggcggagccg gacttcttcg
      901 tgcccgagga cgccgcgcgc aagttcggcg aggccgtcga gcgcggcgcg aagctcgagc
      961 gcgactggca ggcccgcttc gaggcctacc gccaggcgta tcccgacctc gcgcgcgagc
     1021 tcgacgaccg cctgcacggt cggctgccgg acggctggca gcaggcgctg cccgtcttcc
     1081 cggccgacga caagggcatg tcgacgcgcg tcgcgggcaa gaagattctc gcggcgatcg
     1141 ggccgaagct gccggcgctg gtgggcggct ccgccgacct cgatccgtcg accttcacgc
     1201 cgctcgtggg cgccggcgac ttcggcccgc cgcccgcggc ggacgcgaag gcgatcgagg
     1261 gcaccgacgc cggcggcatc tcgtacgcgg gccgcaacct gcacttcggc gtgcgcgagc
     1321 atgcgatggg cgcgatcgtc aacggcctgg ccgcgcatgg cggcttcgtg ccctacggcg
     1381 cgaccttcct gatcttctcg gactacatgc gacccgcgat ccgcctggcc gcgctgcaga
     1441 agctgcatgc gctgcaggtg ttcacgcacg actcgatcgc gctcggcgag gacggtccca
     1501 cccaccagcc ggtcgagcag ctgatgagcc tgcgcgccat ccccggcctg acgacgatcc
     1561 ggcccgccga cgccaacgag accgcggtcg cctggcgcgt cgccctcgag gcgaaggacc
     1621 gcccggtgct gctcgtgttc acgcgccagg acgtgccgac cctcgaccgc acgcgacacg
     1681 cgagcgccga gggtctcgcc aagggcgcgt acgtgctcgc cgatgccgag ggcggcgcgc
     1741 ccgagatcgt gctgatcgcg agcggctcgg aggtcgggct gatcgtcgcg gcggcgagga
     1801 cgctcgccgc agagggcgtg cgcgcgcgct gcgtgtcgat gccctgctgg gacctgttcg
     1861 cgcagcagcc gaaggagtac cgcgacgcgg tgctgccgcc gtcggcgaaa cggcgcctcg
     1921 ccgtcgaggc gggcacgacg ctcggctggg agcgctgggt cggcgacgag ggcgacgtca
     1981 tcgggctcga ccacttcggc gcgtcggccc ctgcgaagaa gctgctcgcg gagttcggct
     2041 tctcgcccga gcacgtcgtc gagcgcgcgc gccggctgct gcgacgttga tcgacatgga
     2101 cgccgccagg gcgccggccg cgcccgacgc gccctgcggc gcgccgccgc
//
LOCUS       DASVLN010000744        11075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_199838, whole genome shotgun sequence.
ACCESSION   DASVLN010000744 DASVLN010000000
VERSION     DASVLN010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11075
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199838"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1009)
                     /locus_tag="VF453_17625"
     CDS             complement(116..1009)
                     /locus_tag="VF453_17625"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7689532.1"
                     /translation="MANPFQVICLSATPPDLFTSAFGPFVVNPCRNLGELSEALRAGA
                     CDALLIETRTPGDHERLLAWPGLAHAVIDAAVVVVSPEPTPAQCLKLLQVGVQDVVAR
                     REGGDDSLGRVLRIAIERKRMDASAKRAFSTDLTTGLPTHAQLLEHMTHLLALREREP
                     AAMALIVLRLEGFRAAEQALGAEAANVLRRKAAVRLRAALRASDVVASLGGDMYGVLL
                     AWIDAEADAEGVARKLLSSLRQPFNVAGQDLPVMGSVGIGQYPAHGRDAGSLMRRAMS
                     QASNGNVSELGRVGAGAANDE"
     gene            1145..2020
                     /locus_tag="VF453_17630"
     CDS             1145..2020
                     /locus_tag="VF453_17630"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7689533.1"
                     /translation="MPSQRRPALAEMGRVLDRCPESDPATLPGGVRVTRPWSHGELHA
                     DTQPMREHVLIAYRDGPAQRICRSVGRERRVAATLPGTVTIIPAGQAAHWDIDGAIAV
                     SHLYLPPARLPAASQDGRPVELLDRLAVDDPTLVELMAIVARESQAGDAPARLLVEQA
                     LDLLALQLARRHAAHPQAPPPARGGLAAWQLRRVVDCMQARLAEPVTLDELAALAGLS
                     RYHFCAAFQRTMGAPPHRWLRALRMKEARRLLADPRLSVLEVALSVGYETPSAFARAF
                     RAAEGLNPADFRRRR"
     gene            2138..2899
                     /locus_tag="VF453_17635"
     CDS             2138..2899
                     /locus_tag="VF453_17635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7689534.1"
                     /translation="MSLEQSLSGKVAIVTGGARNMGRAFCEAFARMGADVVVHHHAER
                     SAGEAREVAELVRSHGRRALVVCGDLARPAVVREVFDGALAAFGRLDVVVNNAGLVIK
                     KPLSEVTEAEFDRSFGINAKAAFFVMQEAARRLEDGGRIVNMGTTILGATIPGYAVYA
                     GAKAPLEAFTRALAKEVGGRGITVNVVAPGPIDTPFYHGAETPEAIARATTASVAGRL
                     GRVDDVVPVIEFLASPRSQWVTAQTLFVNGGYLAR"
     gene            complement(2961..4619)
                     /gene="treA"
                     /locus_tag="VF453_17640"
     CDS             complement(2961..4619)
                     /gene="treA"
                     /locus_tag="VF453_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreA"
                     /protein_id="HEX7689535.1"
                     /translation="MNQRSVSTIAAARRFVATAMAAFALASPCQADTVVGQTPPPSPD
                     KLYGQLFVDVQSQQVYPDQKTFVDAVPKAPPAQILARYAAQKGQPGFSLADFVARWFV
                     PPSQASVTPPAGQTLREHIDWLWPRLTRTTTTAPDDSSLLPLPEPYVVPGGRFREGYY
                     WDTWFTMLGLQESGHEDLVDDMLDNFAYEIDMFGHIPNGNRTYYLSRSQPPFFALMVE
                     TAARQEGDRAYSRYLPQLRKEYAYWMRGADGLRRGEAFDNVVRLPDGATLNRYWDDED
                     TPRPESWLQDTTTARQAAGRPAAEVWRDLRAAAESGWDFSSRWLGDGVTLATIRTTAI
                     VPVDLNALMFQLERTIARGCLVTHDFACFVRYSAAAVRRADAIDHWLWNPDGYYADYD
                     WQAKRIRADRTAAMLYPLFVRAARPERGRITLRVAEASLLDVGGLSTTTARTGQQWDA
                     PNGWAPLHWIAVQAGRRYGRPDFARAVGTRFLADVQTLYDLDGKLVEKYLVDGSGVGG
                     GGGEYPLQDGFGWTNGVTLELLDLYGNGSASQGRPRSEARLPAD"
     gene            4951..6522
                     /locus_tag="VF453_17645"
     CDS             4951..6522
                     /locus_tag="VF453_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7689536.1"
                     /translation="MERLANMRIGTRLAVGFGFVLALLMLTALFAAAEVRRVGATAQR
                     IVEVDYTHIALAHQIDRGINSQANDLRSAVLAAADEDAAKAYITRVSDATRELGKTKQ
                     ALAALPTTDRGRALLAHLQETGDNYVRLRSRTVTLLQAHLPDVARNYLLKDLKAPQEA
                     YLAASRELVDYYEGVMQSASADASSTVRTAIAITLALAAVALAGGIAISLLISRTITR
                     GLQRAVRIAETVATGDLSSRIQVRGRDEVAQLLQALGTMNDSLVALVGTVRESSDSIE
                     TGSREIASGNADLSRRTESQASNLQQTGASMEELTATVRQSAATAQQASELAGAASGV
                     AESGGEAMVRVVETMERIARSSRKVVDIIGVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRSLAQRSANAAREIKGLIADSVESVESGSRLVSDAGETMQAIVERVRNV
                     ATLIDEISTATREQTAGLVDINDAVGAIDKMTQQNSALVEQSAAAAESLRGQASRLVH
                     AVSAFKLREEAGAGA"
     gene            6681..8195
                     /locus_tag="VF453_17650"
     CDS             6681..8195
                     /locus_tag="VF453_17650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689537.1"
                     /translation="MSADLLDRIADQIPSMLAYWDADLRCRYANQAYRTWFGIEPQAL
                     VGRTLPELLGPELYRLNRPHIEQALAGVEQRFERDVPGPSGVRRSIAHYLPDRVDGRV
                     AGFIAHVIDVTPLKVAEDRLRVARRRTAMSALDLRRRAAALREAQRLAQVGSWEWQFA
                     ADRLVWSPELCRIFGLAPNATPPPYAEQGALFLPASWATLQQAVSTTRAGGAPYTLEL
                     EFHHPDGSVHMIEARGEAIRDAAARVVGLRGTVHDITLKRRLDEARVRAQVAEAANRL
                     KTELLSRASHELRTPLNAIIGFGSLLRESAGLPADQLQWVDHIIDGGRHLLALTNDLL
                     DLSASETGQLSLTLETVDLEDVATRVCRMMEPEARRAGMRIEQKPAPETSRLVKADSV
                     RARQIVTNLLSNAIKYGREGTAVMLSIEAAPPGMRSLHVEDTGPGLTEVQLALLFKPF
                     ERLDAPRTTVPGAGLGLAVSQQLARLMNGRVEVRSEPGVGSCFTLVLPAAGDRD"
     gene            8329..8628
                     /locus_tag="VF453_17655"
     CDS             8329..8628
                     /locus_tag="VF453_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689538.1"
                     /translation="MYRFIRTATVKNAALLPSALGFASQVTAHLNKAHALNMRFGAEQ
                     FGGANIHWHFDADSLDKMQHINETLMEDREYNTLLNKFKDTWAEGSMRDTVIRLI"
     gene            8634..9179
                     /locus_tag="VF453_17660"
     CDS             8634..9179
                     /locus_tag="VF453_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX7689539.1"
                     /translation="MKQALQIRFVGMEPSAALELPIRRAVAKLEGFHPHLMSCRVTVE
                     ELDKHKHQGRQHAVRIDLTVPGAELCVDHVRHEDVRLAVRDAFADLRRQLQDVVRRTQ
                     GHVKVHPVEESGEIVRFDIEGRYGFIRSDDGEEYWFGPDNVVDGRFDQLLPGTEVRFV
                     PEFAAEGRQAKRVNLGHHHVG"
     gene            9184..>11075
                     /locus_tag="VF453_17665"
     CDS             9184..>11075
                     /locus_tag="VF453_17665"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7689540.1"
                     /translation="MPAAQQEAAPQVVEAPTSGGADAPSAPADAGALLDVVRELVAEL
                     GAGRAAPLSLDDTLESDLGIDSLARMELGLRIERGLGRRIGDPALLEAESVRDLLRAL
                     RAAPPGSRPLVKGETAPSPPPAADGDAVALPSGARTLVDVLQWHALRTPERTHVKLLR
                     QGEPIAELTHASLHRRARGLAQALREFGVARGDTVALMLPTGAPMLECFMGVLVAGAT
                     PVPIYPPARAREIEEHLRRQSGILGSARATLLVCEASALPAARILRLGVRSLRAAVTP
                     EDLVSRSGARASTDLPGESDVALVQYTSGSTGQPKGVVLTHANLLADIRAMGEAIGAT
                     SADVFVSWLPLYHDMGLIGAWLGCLYHGIPLVLMPPQDFLARPARWLRAIDDHRGTLT
                     AGPNFAYDIVATRVPDGELEGLDLRCLRGAMNGSEAVRAATLEAFCARFERRGFDRRA
                     LWPVYGLAECGVGLTFPPPGRGPRIDRIDPRGLRERGVAVPSVEASAASVVCCGMPLP
                     SHELRVVDDRGSELPDRREGRIQFRGPAATSGYLRNAEATRRLFDGDWIETGDLGYVA
                     DGELFVTGRLKDMIKHAGHNLHPDDLESAVGALPGVRRGCVAVFGSDDAGPRGERIVV
                     VAETRL"
BASE COUNT         1591 a         4045 c         3939 g         1500 t
ORIGIN      
        1 cggcggatcg ggacggcgtt cccgcctcag gcccggcgag ccggccagca ccggcgagcg
       61 cgctcttgcc ggggccggca caaggcgatc gcgccggctg cctcgcggtg gcgcatcact
      121 cgtcgttcgc cgccccggcc cccacgcgcc ccagctcgga cacgttcccg ttggacgcct
      181 ggctcatcgc gcgccgcatc agcgagccgg cgtcgcggcc atgggcgggg tactggccga
      241 tgcccacgga gcccatcacc ggcaggtcct ggccggcgac gttgaagggc tggcgcagcg
      301 aggacaggag cttgcgggcc acgccctcgg cgtcggcctc ggcgtcgatc caggcgagca
      361 ggacgccgta catgtcgcca ccgagcgacg ccacgacgtc gctcgcgcgc agcgccgcgc
      421 gcaggcgcac cgcggccttg cgccgcagca cgttcgcggc ctcggcgcca agcgcctgct
      481 ccgcggcgcg gaagccctcg agccggagca cgatgagcgc catcgcggcc ggctcgcgct
      541 cgcgcagcgc cagcaggtga gtcatgtgct cgagcagctg cgcgtgcgtg ggcaggcccg
      601 tcgtcaggtc cgtgctgaag gcgcgcttgg cgctcgcgtc catgcgcttg cgctcgatgg
      661 cgatgcgcag cacgcggccg agcgagtcgt cgccgccctc gcgccgcgcg acgacgtcct
      721 gcacgcccac ctgcagcagc ttcaggcact gggcgggcgt cggttcgggc gagacgacga
      781 ccaccgccgc gtcgatcacc gcatgcgcga ggccgggcca ggcgagcagg cgctcatggt
      841 cgccgggcgt gcgggtctcg atcagcaacg cgtcgcaggc accggcgcgc aaggcctccg
      901 acagctcgcc gaggttgcgg cacggattga cgacgaacgg cccgaaggcc gaggtgaaca
      961 gatccggcgg cgtcgcactg agacagatga cctggaacgg atttgccatg ggacgccgga
     1021 cttcgagttg tttccatcat gcagcagttt tgctgcactg cacaggcatg gaaacccggc
     1081 cgcccgacag gggcgggctg cctagaatcg cgccgtcgga accgcccgga cgaaagctgt
     1141 cgcgatgcct tcacaacgcc ggcccgccct cgccgagatg gggcgcgtcc tcgatcgctg
     1201 ccccgagtcg gacccggcga ccttgccggg cggcgtccgc gtcacccgcc cgtggtcgca
     1261 cggcgagctc cacgccgaca cccagccgat gcgcgagcac gtgctcatcg cctaccgcga
     1321 cggcccggcg caacgcatct gccgctcggt ggggcgcgag cgccgggtcg cggcgacgct
     1381 tcccggcacg gtcaccatca tcccggccgg ccaggcggcg cactgggaca tcgacggcgc
     1441 catcgcggtc tcgcacctct acctgccgcc cgcgcgcctg cccgcggcct cgcaggacgg
     1501 ccgccccgtg gagctgctcg accgcctggc cgtcgacgat ccgacgctgg tcgaactgat
     1561 ggcgatcgtc gcgcgcgagt cgcaggccgg cgacgccccg gcccgcctgc tggtggagca
     1621 ggcgctcgac ctgctcgcgc tgcagcttgc ccggcgccac gccgcacatc cgcaggcgcc
     1681 gcccccggcc cgcggcgggc tcgcggcctg gcagctgcgc cgcgtcgtcg actgcatgca
     1741 ggcgcggctc gcggagccgg tgacgctgga cgaactcgct gcgctcgccg gcctgagccg
     1801 ctaccacttc tgcgcggcgt tccaacggac gatgggcgcg ccgccgcacc gctggctgcg
     1861 cgcgctgcgg atgaaggagg cgcggcggct gctggccgat ccgcggctgt cggtgctcga
     1921 ggtcgcgctg agcgtcggct acgagacgcc gtcggcgttc gcccgcgcct tccgggcggc
     1981 cgagggcctg aaccccgcgg acttccggcg ccggcggtag gcgcggcccc caggccgtcg
     2041 caagcgcggc gtggattgcg caatgccggc gcgcaacgcc cgcgcgcccg gccctaccct
     2101 gcgcaatccc atccaccctc atcgaccagg agcgcgcatg tccctcgagc aatccctttc
     2161 aggcaaggtc gcgatcgtca ccggcggcgc gcgcaacatg ggccgcgcgt tctgcgaggc
     2221 gttcgcgcgc atgggcgccg acgtcgtggt gcaccaccac gccgagcgct cggccggcga
     2281 ggcgcgggag gtcgccgagc tcgtgcgctc gcacggccgg cgcgcgctcg tcgtgtgcgg
     2341 cgacctcgcg cgcccggccg tcgtgcgcga ggtcttcgac ggcgcgctgg ccgccttcgg
     2401 ccggctggac gtcgtcgtca acaacgcggg actggtgatc aagaagccgc tgtccgaagt
     2461 gaccgaagcc gagttcgacc gctcgttcgg catcaacgcc aaggcggcgt tcttcgtgat
     2521 gcaggaagcc gcgcgccggc tcgaggacgg cggtcgcatc gtcaacatgg gcacgacgat
     2581 cctcggcgcg acgatcccgg gctacgcggt ctacgcgggc gccaaggcgc cgctggaggc
     2641 gttcacgcgc gcgctggcca aggaggtcgg cggccgcggc atcaccgtca acgtcgtcgc
     2701 cccggggccg atcgacacgc ccttctacca cggcgccgag acgcccgagg ccatcgcccg
     2761 cgccaccacg gccagcgtcg ccggccggct ggggcgggtc gacgacgtgg tgcccgtgat
     2821 cgagttcctc gcctcgccgc gctcgcaatg ggtgacggcg cagaccctgt tcgtcaacgg
     2881 cggctacctc gcccgctgac gccgacggcc tcgcccggct gcgcgcgccg ggttcgtccc
     2941 ggcgccttcc ggccgccgcg tcaatccgcc ggcaggcgcg cctcggagcg cggccggccc
     3001 tggctcgcgc tgccgttgcc gtagaggtcg agcagctcga gcgtcacccc gttggtccag
     3061 ccgaagccgt cctgcagcgg atattcgccg ccaccgccgc ccacgccgct gccgtcgacg
     3121 aggtatttct cgaccagctt gccatccagg tcgtacagcg tctgcacgtc ggcgaggaag
     3181 cgcgtgccga cggcgcgtgc gaagtcggga cggccgtagc ggcgtcccgc ctgcacggcg
     3241 atccagtgca ggggcgccca gccgttcggc gcgtcccact gctggccggt gcgggccgtc
     3301 gtcgtcgaca ggccgcccac gtcgagcagg gacgcctcgg ccacgcgcag cgtgatgcgt
     3361 ccgcgctcgg gccgcgccgc ccgcacgaac aacgggtaca gcatcgcggc cgtgcggtcg
     3421 gcgcggatcc gcttcgcctg ccagtcgtag tccgcgtaat agccgtccgg attccacaac
     3481 caatggtcga tcgcatcggc ccggcgcacc gccgccgccg aatagcggac gaagcacgcg
     3541 aagtcgtgcg tgaccaggca gccgcgcgcg atggtgcgct cgagctggaa catcagcgcg
     3601 ttgaggtcga ccgggacgat ggcggtggtg cggatcgtcg ccagcgtgac cccatcgccg
     3661 agccagcgcg agctgaagtc ccagccgctc tcggccgccg cgcgcaggtc gcgccagacc
     3721 tcggcggccg ggcggcccgc cgcctgccgg gccgtcgtcg tgtcctgcag ccaggactcg
     3781 ggacgcggcg tgtcctcgtc gtcccagtag cggttgagcg tcgcgccgtc gggcaggcgc
     3841 acgacgttgt cgaacgcctc gccgcgccgc aggccgtccg cgccgcgcat ccaatacgcg
     3901 tattccttgc gcaattgcgg caggtagcgc gaataggccc ggtcgccttc ctgtcgcgca
     3961 gcggtctcga ccatcaatgc gaagaacggc ggctgcgaac ggctgaggta ataggttcga
     4021 ttgccgttcg ggatgtggcc gaacatgtcg atctcgtacg cgaaattgtc gagcatgtcg
     4081 tcgacgagat cctcgtgccc ggattcctgc agtcccagca tcgtgaacca ggtgtcccag
     4141 taatagcctt cgcggaaccg gccgcccggg acgacgtagg gctcgggcag cggcagcagc
     4201 gagctgtcgt cgggtgccgt cgtcgtggtg cgcgtgagcc ggggccacag ccagtcgatg
     4261 tgctcgcgca gcgtctggcc cgcgggcggc gtgaccgacg cctgcgacgg cggcacgaac
     4321 cagcgcgcga cgaagtccgc gagcgagaag cccggctgtc ccttctgcgc ggcgtaccgg
     4381 gcgaggatct gcgccggcgg cgccttcggc acggcgtcga cgaaggtctt ctggtcggga
     4441 tagacctgct gcgactgcac gtccacgaac aactggccgt agagcttgtc gggcgacggc
     4501 ggcggcgtct ggccgacgac ggtgtcggcc tggcagggcg acgccagcgc gaatgcggcc
     4561 attgcggtgg cgacgaatcg gcgcgcggcc gcaattgtcg agacggatcg ttgattcatg
     4621 atcgttcgca tccctttgga gacgcgaaac gctatcacgg gcgaatcgtg ccgattcgat
     4681 aacccatccc tcaggcgggc acgacgccaa tatgtcgaat atttccgaat cagccgatgt
     4741 tgcatccggt ttcgctgccc gggattcgcg cgcaattcca gcgcgacagg ccgccgccga
     4801 gccggagcca tgccgaaccg gctgcctgac atgtcttttc cggcattggg gttcgcgttc
     4861 ctcaagcgcg ccgggcgatg ggacgaaaca aggcgaaggc cgcggtacgc ggcgcctccc
     4921 acgacaacca tcacgacgag agacatacag atggaacgcc tcgccaacat gcgcatcggc
     4981 acccggctcg cggtcggatt cggcttcgtg ctcgccctgc tgatgctcac cgccctgttc
     5041 gccgccgccg aggtgcgccg ggtcggcgcc accgcccagc gcatcgtcga ggtggactac
     5101 acgcacatcg cgctggccca ccagatcgac cgcggcatca actcgcaggc caacgacctg
     5161 cgcagcgccg tgctcgccgc cgccgacgag gacgccgcca aggcctacat cacccgggtc
     5221 agcgacgcga cgcgcgagct cggcaagacc aagcaggcgc tcgccgcgct gccgaccacc
     5281 gaccgcggcc gcgcgctgct cgctcatctc caggagaccg gcgacaacta cgtgcgcctg
     5341 cgctcgcgca ccgtgacgct gttgcaggcc cacctgcccg acgtcgcgcg caactacctg
     5401 ctcaaggacc tgaaggcgcc gcaggaggcc tacctggccg cgtcgcgcga gctcgtcgac
     5461 tactacgagg gcgtgatgca gtccgcctcc gccgacgcta gctcgaccgt gcgcaccgcc
     5521 atcgcgatca cgctggccct cgcggcggtc gcgctggccg gcggcatcgc gatcagcctg
     5581 ttgatctcgc gcacgatcac gcgcgggctc caacgcgccg tgcgcatcgc ggagacggtg
     5641 gccaccggcg acctgagctc acgcatccag gtgcgcggac gcgacgaggt cgcgcagctg
     5701 ctgcaggcgc tgggcacgat gaacgacagc ctcgtcgcgc tcgtcggcac ggtgcgcgag
     5761 agctccgaca gcatcgagac cggctcgcgc gagatcgcca gcggcaacgc cgacctgtcc
     5821 cgccgcacgg agagccaggc ctccaacctg cagcagaccg gcgcgtcgat ggaggagctc
     5881 accgccaccg tgcgccagag cgccgcgacc gcgcagcagg cgagcgagct cgccggcgcg
     5941 gccagcggcg tcgccgagag cggcggcgag gcgatggtcc gcgtcgtcga gacgatggag
     6001 cgcatcgccc gcagctcgcg caaggtggtc gacatcatcg gcgtgatcga cggcatcgcg
     6061 ttccagacca acatcctcgc gctgaacgcg gcggtcgagg cggcgcgcgc gggcgagcag
     6121 ggccgcggct tcgccgtcgt cgcgggcgag gtgcgcagcc tcgcgcagcg ctcggccaac
     6181 gcggcgcgcg agatcaaggg gctgatcgcc gacagcgtcg agagcgtcga atccggcagc
     6241 cgcctcgtga gcgacgccgg cgagacgatg caggcgatcg tggagcgcgt gcgcaacgtc
     6301 gccacgctga tcgacgagat cagcaccgcg acccgcgagc agaccgcggg cctcgtcgac
     6361 atcaacgacg cggtcggcgc gatcgacaag atgacgcagc agaactcggc gctggtcgag
     6421 cagtcggccg ccgccgccga gagcctgcgc gggcaggcct cgcggctcgt gcatgcggtg
     6481 agcgcgttca agctgcgcga ggaagcgggc gccggggcct gagaaggttc gccgcgcgcg
     6541 acgtcattcg cgcgtcgatt gcgtccgcgc aacgcgtcgg gcagcggtgc atccgaggat
     6601 gcccacggtc gcgccagtcc ggcgcgacgc cgtcgcgcac gcgatcgccg ggcgcgaggc
     6661 gtgcgtgccg cgggagcgcc atgtccgccg accttctcga tcgcatcgcc gaccagatcc
     6721 cgtccatgct cgcctattgg gacgcggacc tgcgatgccg gtatgccaac caggcctatc
     6781 gcacctggtt cggcatcgag ccccaggccc tcgtcgggcg cacgctgccc gagttgctcg
     6841 gcccggagct ctaccggctc aaccgtcccc acatcgagca ggcgctcgca ggcgtcgagc
     6901 aacgcttcga gcgcgacgtc cccggcccgt cgggggtgcg tcgcagcatc gcccactacc
     6961 ttcccgaccg ggtggacggg cgggtcgccg ggttcatcgc ccatgtcatc gatgtcaccc
     7021 cgctgaaggt cgcggaggac cggctgcgag tcgcgcgtcg ccgcacggcc atgtccgcgc
     7081 tcgacctgcg caggcgcgcc gccgccctgc gcgaggcgca gcgcctggcg caggtcggca
     7141 gctgggaatg gcagttcgcg gcggatcgcc tggtctggtc gcccgagctc tgtcggatct
     7201 tcggcctggc gccgaatgcc acgccgcccc cttacgcgga acagggcgcg ctcttcctgc
     7261 cggcgagctg ggcgacgctg cagcaggccg tttccacgac gcgggccggc ggcgcgccct
     7321 acacgctcga gctggagttt caccatcccg acggttccgt gcacatgata gaagcccgcg
     7381 gcgaggcgat ccgcgacgcg gccgcgcgcg tggtcggact gcgcggcacc gtgcacgaca
     7441 tcaccttgaa gcgccggctg gacgaggcgc gggtccgcgc gcaggtcgcc gaggcggcca
     7501 accgcctcaa gacggagctg ctgtcccgcg ccagccacga gctgcgcacg ccgctgaacg
     7561 ccatcatcgg cttcggcagc ctgctgcggg aaagcgccgg actgccggcc gaccagctgc
     7621 aatgggtgga tcacatcatc gacggcggca ggcacctgct cgcgctcacc aacgacctgc
     7681 tcgatctctc cgcctccgaa acggggcagc tctccctgac cctggagacg gtcgacctcg
     7741 aagacgtcgc gacacgcgtc tgccggatga tggagccgga ggcccggcga gcggggatgc
     7801 gcatcgagca gaagccggcg cccgagacga gccgcctcgt caaggccgac agcgtgcgcg
     7861 cacgccagat cgtgacgaac ctgctgtcga acgcgatcaa gtacggccgc gagggcacgg
     7921 cggtcatgct gtcgatcgag gcggcgccgc ccggcatgcg cagcctgcac gtcgaagaca
     7981 ccggcccggg cttgaccgaa gtccagctcg cgctgctctt caagcccttc gagcggctcg
     8041 atgcgccgcg gacgaccgtc ccgggcgccg gactcgggct ggccgtgtcg caacaactgg
     8101 cgcgactgat gaacggccgc gtcgaggtgc gcagcgagcc gggcgtgggc agttgcttca
     8161 cgctcgtgct tccggccgcc ggcgatcggg actgagtccc ccgatcgacg gccagcttca
     8221 cagcttcatt gagtgcgcgc aaccggcgcc ggcgcgacgc gacgagcatg caaaggatgc
     8281 cggccgatcc ggacggcgtc gcttcaccca ctcaccagga gcacggagat gtacaggttc
     8341 atccgcaccg ccaccgtcaa gaatgccgcg ctgttgccct cggccctcgg gttcgcctcc
     8401 caggtgacgg cccacctcaa caaggcccat gccttgaaca tgcgcttcgg cgcggaacag
     8461 ttcggcggcg ccaacatcca ttggcacttc gatgccgaca gcctcgacaa gatgcagcac
     8521 atcaacgaga cgctgatgga ggatcgcgaa tacaacacct tgctcaacaa gttcaaggac
     8581 acgtgggccg aaggctcgat gcgcgacacc gtgatccgcc tgatctgagt ccgatgaagc
     8641 aagcgctgca gatccgtttc gtggggatgg agccctccgc ggcgctggaa ctcccgatcc
     8701 ggcgcgcggt cgcgaagctc gaagggttcc acccgcacct gatgtcgtgc cgcgtgacgg
     8761 tcgaggagct ggacaagcac aagcaccagg gccggcagca cgcggtgcgc atcgacctga
     8821 cggtgccggg cgcggaactg tgcgtggacc acgtgcgcca cgaggacgtc cgcctggccg
     8881 tgcgcgacgc gttcgccgac ctccgccggc agctgcagga cgtcgtccgg cggacccagg
     8941 gccacgtgaa ggtccatccc gtcgaggaaa gcggcgagat cgtgcggttc gacatcgagg
     9001 ggcgctacgg gttcatccgg tccgacgacg gagaggaata ctggttcggg cccgacaacg
     9061 tcgtcgacgg ccggttcgac cagctgctgc ccggcaccga agtgcgcttc gtcccggagt
     9121 tcgccgccga aggccgccag gccaagcgcg tgaacctcgg ccatcatcac gtgggctgaa
     9181 gccttgccgg ccgcgcagca ggaggcggcc ccgcaggtcg tcgaggcgcc cacctcgggc
     9241 ggcgcggacg ccccgtccgc gcctgccgac gcgggcgcac tgctcgacgt ggttcgcgag
     9301 ctggtcgccg agctgggggc cggtcgcgcg gcaccgttgt ccctggacga cacgctcgaa
     9361 tccgacctgg gcatcgacag cctcgcgcgc atggagctgg ggctgcggat cgaacgcggc
     9421 ctgggccggc gcatcggcga cccggccctg ctcgaagccg aatcggtgcg cgacctgctg
     9481 cgcgcgttgc gagccgcgcc gccggggtcg cgcccactgg tgaagggaga gacggctcca
     9541 tccccgcccc cggcggcgga cggcgacgcg gtcgcgctgc cgtccggcgc ccgcacgctc
     9601 gtcgacgtct tgcagtggca tgcgctgcgc accccggagc gcacccacgt gaagctgctg
     9661 cggcagggcg aaccgatcgc cgagctgacg catgcgtcgc tgcaccgccg cgcccgcggc
     9721 ctcgcgcagg ccttgcgtga gttcggcgtg gcgcgcggcg acacggtggc gctgatgctg
     9781 ccgaccgggg caccgatgct cgagtgcttc atgggcgtgc tggtcgccgg ggccacgccg
     9841 gtaccgatct atccgcccgc tcgcgcgcgc gagatcgagg aacacctgcg gcggcagtcc
     9901 gggatcctcg gcagcgcacg cgcgacgctg ctggtgtgcg aggcctcggc cctgcccgcg
     9961 gcacgcatcc tgcggctggg cgtgcgcagc ctgcgggccg cggtcactcc ggaggacctg
    10021 gtgtcgcgtt ccggcgcgcg cgcatcgacc gacctgccgg gcgagagcga cgtcgcgctg
    10081 gtgcagtaca cctcgggcag caccggtcag cccaagggcg tcgtgctgac gcatgcgaac
    10141 ctgctggccg acatccgcgc gatgggcgag gcgatcggcg ccacatcggc ggatgtcttc
    10201 gtgagttggc tcccgctgta ccacgacatg ggcctgatcg gcgcctggct tggctgcctc
    10261 taccacggca tcccgctggt gctgatgccg ccgcaggact tcctcgcgcg cccggcgcgc
    10321 tggctgcgcg cgatcgacga ccatcgcggg acgctgacgg cggggcccaa cttcgcctac
    10381 gacatcgtgg cgacccgcgt gccggacggc gagctcgagg gcctcgacct gcgctgcctg
    10441 cgcggggcga tgaacgggtc ggaggccgtc cgcgccgcga cgctggaagc cttctgcgcg
    10501 cgcttcgagc gccgcggctt cgaccggcgc gccctgtggc cggtctacgg attggcggaa
    10561 tgcggcgtgg ggctcacgtt tccgccgccc gggcgcgggc cgaggatcga ccgcatcgat
    10621 ccccggggct tgcgcgagcg cggcgtggcg gtgccctcgg tcgaggcgtc cgccgcgtcc
    10681 gtcgtctgct gcgggatgcc cttgccgtcg cacgaactgc gggtcgtcga cgaccgcggc
    10741 tcggagctgc cggatcgccg cgaggggcgc atccagttcc gcggcccggc agcgacatcg
    10801 ggctacctgc gcaatgccga ggcgacccgg cgcctgttcg acggcgactg gatcgagacg
    10861 ggcgacctgg gctacgtggc ggacggcgag ctgttcgtga ccgggcgcct caaggacatg
    10921 atcaagcatg ccggccacaa cctccaccct gacgacctcg agtcggcggt gggtgcgctg
    10981 cccggcgtgc gccgcggctg cgtcgcggtc ttcggcagcg acgacgccgg cccgcggggc
    11041 gagcggatcg tggtcgtcgc ggagacgcgc ctgca
//
LOCUS       DASVLN010000745         5210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_37672, whole genome shotgun sequence.
ACCESSION   DASVLN010000745 DASVLN010000000
VERSION     DASVLN010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37672"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VF453_17670"
     CDS             <1..538
                     /locus_tag="VF453_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7689541.1"
                     /translation="CGVARGAVRVSSVSLSGKQVSLTYVEPGTWFGDIALFDGLGRTH
                     DADAHGATTLLSVRKADFKELLAQYPELYDALLRLNCRRLRIMFNLIEDLNTRPLSAR
                     LAKNILVLARSYGVQQGEEVRIGLQLAQEDLAQLVGASRQRVNQELKELEREGALRVE
                     PTRLVVLSREKLIHAAER"
     gene            597..1685
                     /locus_tag="VF453_17675"
     CDS             597..1685
                     /locus_tag="VF453_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7689542.1"
                     /translation="MTDQTEQNTGTRPVAESHRFDLAALEAHLARHLPGFAGPLSVEQ
                     FKGGQSNPTYKLSTPARAYVMRSKPGPVAKLLPSAHAIEREYRVMDALAATDVPVARM
                     LLLCEDESVIGRAFYVMEHVEGRVLWDQGLPGATPAERAAIYDEMNRVIAALHRVDPT
                     AIGLADYGKPGNFFARQIGRWTKQYQASITDPIEAMDRLIDWLPKHMPASALDESQVA
                     IAHGDFRMDNLIFHPAEPRVLAVLDWELSTLGHPLGDFSYHCMAWHIPPGTFRGIGGL
                     DHAGLGIPAERDYVRRYCERTGRADPDALLADWNFYLAYNLFRLAAILQGIARRVVDG
                     TASSAQARATGAGARPLAELAWQFAQKA"
     gene            1714..2943
                     /locus_tag="VF453_17680"
     CDS             1714..2943
                     /locus_tag="VF453_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7689543.1"
                     /translation="MDFAYSARTLALQERLRAFMAEHVVPNEARYHAEIEANTQAGRR
                     WTPLALIEELKAKARAAGLWNLFLPDSERGAGLSNADYAPLAETMGRTPWASEVFNCS
                     APDTGNMEVLVRYGTPGQQARWLEPLLEGRIRSAFAMTEPAVASSDATNIESRIVRDG
                     DDYVLNGRKWWTSGAGDPRCAVYIFMGKSDPDAPPHAQQSMILVPADAPGVKVLRPLN
                     VFGYDDAPHGHMEVAFDDVRVPVSNILLGEGRGFEIAQGRLGPGRIHHCMRLVGVAER
                     ALELMCERAIARVAFGKAIAQQTVTQERIAEARCRIDMARLLTLKAAWMMDVAGNKAA
                     KAEIAMIKVVAPKMACDVIDWAMQVFGGMGMSDDAPLAYFYTLARTLRFADGPDEVHR
                     NAIAKIELGKHAPGARG"
     gene            complement(2947..3570)
                     /locus_tag="VF453_17685"
     CDS             complement(2947..3570)
                     /locus_tag="VF453_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689544.1"
                     /translation="MPAWPQLAAWARENGWSLKRTIAHDGWVMDHEPGHPGWRVEWGP
                     AQRGFMSSHELRIRLETPRLPENMQALVLDRPLLARLDREVYQQITDSVQTRLDEETP
                     EEMRWLAMHAKLSPNQMGVPLRDRYGGLCSEPEWLSRWLAGPVSAALKKRADVMPVEA
                     VSAEPFALRLARGQMVLRQAAPALAMPAFVASMAVAEAVDATLGAVR"
     gene            3658..4353
                     /locus_tag="VF453_17690"
     CDS             3658..4353
                     /locus_tag="VF453_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7689545.1"
                     /translation="MIEVYSWATPNGHKVHIMLEECGLPYRANPVDIGAGDQFKPDFL
                     RISPNNKIPAIVDPDGPDGRPISLFESGAILLYLAGKTGRFLPDSTSERYEMLQWLMF
                     QVGCVGPMLGQAHHFRVYAPEKIAYAVERYTNEARRLYGVMDRRLAKSRYLGGRDYGI
                     ADMAVFPWLRSWKNQGIAWADFPHLKGWFDEIAARPAVRRGVEVLAGLRKPLDNDARA
                     REVLFGETQYERR"
     gene            4393..4469
                     /locus_tag="VF453_17695"
     tRNA            4393..4469
                     /locus_tag="VF453_17695"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4427..4429,aa:Arg,seq:tct)
     gene            4515..4991
                     /locus_tag="VF453_17700"
     CDS             4515..4991
                     /locus_tag="VF453_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689546.1"
                     /translation="MAVRTLALALAALLPIAALLAAVAYQPDDAALLRTRTVHGVFEA
                     ERDVHGRFWGVAIAGERVHTSTGPIFPGTDDALGVPPRERVVAEFNDLPTLGGRVAVL
                     VALRGESDGERYLDVPLDERRRRFRHCTLVDIGWSLLLALLFLLVWISAERDAAAR"
BASE COUNT          781 a         1944 c         1766 g          719 t
ORIGIN      
        1 gtgcggcgtc gcgcgcggcg cggtgcgcgt gagctcggtg tcgctctcgg gcaagcaggt
       61 ctcgctgacc tacgtcgagc cgggcacctg gttcggcgac atcgccctct tcgacggcct
      121 gggccgcacg cacgacgccg acgcgcacgg cgcgaccacc ctgctgtcgg tgcgcaaggc
      181 cgacttcaag gagctgctcg cgcagtatcc cgagctctac gacgcgctgc tgcgcctgaa
      241 ctgccggcgc ctgcgcatca tgttcaacct catcgaggac ctgaacacgc ggccgctgtc
      301 cgcgcgcctg gccaagaaca tcctcgtgct cgcgcgcagc tacggcgtgc agcagggcga
      361 ggaggtgcgc atcggcctgc agctcgcgca ggaggatctc gcgcagctgg tgggcgcgtc
      421 gcgccagcgc gtgaaccagg agctcaagga gctcgagcgc gaaggcgcgc tgcgcgtgga
      481 gccgacgcgg ctcgtcgtgc tctcgcgcga gaagctgatc cacgccgcag aacgctgaag
      541 ccgcatcggg catcgtccga tgcgaggtcc cacgacaacg catccgcacc gacccgatga
      601 ccgaccagac cgaacagaac accggcaccc gcccggtcgc cgagagccac cgcttcgacc
      661 tcgccgcgct cgaggcccac ctcgcgcgcc acctgcccgg attcgcgggc ccgctgtcgg
      721 tcgagcagtt caagggcggg cagtccaacc cgacctacaa gctctcgacg cccgcgcgcg
      781 cctacgtgat gcgcagcaag cccgggccgg tcgccaagct gctgccgtcg gcgcacgcga
      841 tcgagcgaga gtaccgcgtg atggacgcgc tggcggccac cgacgtgccg gtcgcacgca
      901 tgctgctgtt gtgcgaggac gagtccgtga tcggccgcgc cttctacgtc atggagcacg
      961 tcgagggccg cgtgctgtgg gaccagggcc tgcccggcgc gacgccggcc gaacgcgccg
     1021 cgatctacga cgagatgaac cgcgtgatcg ccgcgctgca ccgcgtcgac ccgaccgcca
     1081 tcggcctggc ggactacggc aagccgggca acttcttcgc gcgccagatc gggcgctgga
     1141 cgaagcagta ccaggcctcg atcaccgacc cgatcgaggc catggatcgc ctcatcgact
     1201 ggctgccgaa gcacatgccg gcgagcgcgc tggacgagtc gcaggtcgcc atcgcgcacg
     1261 gcgacttccg catggacaac ctgatcttcc atccggccga gccgcgcgtg ctcgccgtgc
     1321 tggactggga gctctcgacg ctcggccatc ctctgggcga cttcagctac cactgcatgg
     1381 cgtggcacat cccgccgggc accttccgcg gcatcggcgg cctcgaccac gcggggctgg
     1441 gcatccccgc cgagcgcgac tacgtgcgac ggtactgcga gcgcaccggc cgcgcggacc
     1501 ccgacgcgct gctcgcggac tggaacttct acctcgccta caacctgttc cgcctcgcgg
     1561 cgatcctgca gggcatcgcg cgccgcgtcg tcgacggcac ggcctcgagc gcgcaggcgc
     1621 gcgcgacggg cgcgggcgcg cgtcccctcg cggagctggc ctggcagttc gcgcagaagg
     1681 cctgagacgg ccgcgacgag aaggagcaac gacatggact tcgcctattc cgcgcgcacg
     1741 ctggccctgc aggaacgcct tcgggccttc atggccgaac acgtcgttcc gaacgaggcg
     1801 cgctaccacg ccgagatcga ggccaacacg caggccggcc ggcgctggac gccgctcgcg
     1861 ctgatcgagg aactcaaggc caaggcgcgc gccgccggcc tgtggaacct gttcctgccg
     1921 gacagcgagc gcggcgccgg cctgtcgaac gcggactatg cgccgctcgc cgagaccatg
     1981 ggccgcacgc cttgggcgag cgaggtcttc aactgctccg ctcccgacac cggcaacatg
     2041 gaggtgctgg tgcgctacgg cacgcccggg cagcaggcgc gctggctcga gcccctcctg
     2101 gaaggccgca tccgcagcgc cttcgcgatg accgagcccg ccgtggcctc gtcggacgcg
     2161 accaacatcg agtcgcgcat cgtgcgcgac ggcgacgact acgtcctgaa cggccgcaag
     2221 tggtggacgt cgggcgccgg cgatccgcgc tgcgcggtct acatcttcat gggcaagagc
     2281 gatcccgacg cgccgccgca cgcgcagcag tcgatgatcc tcgtgcccgc cgacgccccc
     2341 ggcgtgaagg tgctgcgccc gctcaacgtc ttcggctacg acgacgcgcc ccacggccac
     2401 atggaggtcg cgttcgacga cgtacgggta cccgtatcca acatcctgct cggcgagggc
     2461 cgcggcttcg agatcgcgca gggacgcctg ggccccggcc ggatccacca ctgcatgcgg
     2521 ctggtcggcg tggccgagcg cgcgctggag ctgatgtgcg agcgcgcgat cgcgcgcgtg
     2581 gccttcggca aggcgatcgc gcagcagacc gtcacgcagg agcgcatcgc cgaggcgcgc
     2641 tgccgcatcg acatggcgcg gctgctgacg ctgaaggccg cgtggatgat ggacgtcgcc
     2701 ggcaacaagg ccgcgaaggc ggagatcgcg atgatcaagg tggtcgcgcc gaagatggcc
     2761 tgcgacgtca tcgactgggc gatgcaggtc ttcggcggca tgggcatgtc cgacgacgcg
     2821 ccgctcgcct acttctacac gctcgcgcgc acgctgcgct tcgccgacgg gcccgacgag
     2881 gtgcaccgca acgcaatcgc caagatcgag ctcggcaagc acgcgccggg cgcgcggggc
     2941 tgacgctcag cgcacggccc cgagcgtcgc gtcgaccgcc tcggcaaccg ccatcgaggc
     3001 cacgaacgcg ggcatcgcga gcgcgggcgc ggcctggcgc agcaccatct ggccgcgcgc
     3061 cagccgcagc gcgaagggct ccgccgacac ggcctcgacc ggcatcacgt cggcacgctt
     3121 cttcagtgcc gccgacaccg gaccggccag ccaccgcgac agccattccg gctcgctgca
     3181 cagcccgccg tagcggtcac gcagcggcac gcccatctgg ttggggctca gcttggcgtg
     3241 catcgcgagc cagcgcatct cctcgggcgt ttcctcgtcc aggcgcgtct gcacgctgtc
     3301 ggtgatctgc tggtagacct cgcgatccag gcgcgcgagc agcgggcgat ccagcaccag
     3361 cgcctgcatg ttctcgggca ggcgcggcgt ctcgaggcgg atgcgcagct cgtggctgct
     3421 catgaacccg cgctgggccg gcccccactc gacgcgccag ccgggatgcc ccggctcatg
     3481 gtccatcacc cagccgtcat gggcgatggt gcgcttgagc gaccagccgt tctcgcgggc
     3541 ccaggccgcc agctgcggcc atgcaggcat ggaatttccc caggacaacc agcggcggat
     3601 cgcatccagc atgatgttgt aatgttcggc acccgcccct tcccggagga ccttgccatg
     3661 atcgaagtgt attcgtgggc cactcccaac ggccacaagg ttcacatcat gctcgaagaa
     3721 tgcgggctgc cgtatcgagc caatcccgtg gacatcggcg ccggcgacca gttcaagccc
     3781 gacttcctga ggatcagccc gaacaacaag atcccggcca tcgtcgaccc cgacggcccc
     3841 gacggccggc cgatcagcct gttcgagtcg ggcgccatcc tgctgtacct cgccggcaag
     3901 accggccgct tcctgcccga ctccacgagc gagcgctacg agatgctgca gtggctgatg
     3961 ttccaggtcg gctgcgtcgg gccgatgctc ggacaggcgc accacttccg cgtctacgcg
     4021 cccgagaaga tcgcctacgc cgtcgagcgc tacaccaacg aggcacggcg cctgtacggc
     4081 gtgatggacc gccggctggc gaagtcgcgc tacctcggcg gccgcgacta cggcatcgcc
     4141 gacatggccg tcttcccgtg gctgcgctcg tggaagaacc agggcatcgc gtgggccgac
     4201 ttcccgcacc tgaagggctg gttcgacgag atcgccgccc gccccgccgt gcggcgcggc
     4261 gtcgaggtgc tcgcaggcct gcgcaagccg ctggacaacg acgcgcgggc gcgcgaggtg
     4321 ctgttcggcg agacgcagta cgagcgccgc tgaccccgcg ccgtccgcgc cgatagaatc
     4381 gcgggcggcc gactgcccgt agctcaactg gatagagcag ctgccttcta agcagcaggt
     4441 cgggggttcg agtccctccg ggcaggccac gccctctccc gcatcccaac ccgcacgcac
     4501 ccacgacgac gacgatggcc gtccggacgc tggccctggc gctcgccgcc ctcctgccga
     4561 tcgcggcgct gctggcggcg gtcgcctacc agcccgacga cgcggcgctg ctgcgcacgc
     4621 gcaccgtcca cggcgtcttc gaggccgagc gcgacgtcca cgggcgcttc tggggtgtcg
     4681 ccatcgccgg cgagcgcgtg cacacgagca ccggccccat cttcccgggc accgacgacg
     4741 cgctcggcgt cccgccacgc gagcgcgtcg tcgccgagtt caacgacctg cccacgctgg
     4801 gcggccgcgt ggcggtgctg gtggccttgc gcggcgagtc cgacggcgag cgctacctcg
     4861 acgtgccgct cgacgagcgc cgccgccggt tccggcactg cacgctggtc gacatcgggt
     4921 ggtcgttgct gctcgcgctg ctgttcctgc tcgtctggat ctcggccgag cgcgacgcgg
     4981 cggcgcgctg aagccaccgc gtcgacggcg cgcccgttcc gtggtcccat cgcgcatgcc
     5041 ctcgccttcc tccatcgacc tcgctctcga cctcgacctc gaccgccggc gcgccgcgat
     5101 cagctggagc gccggcaagg actcctggct cgcgctgctg cgcgcgcgcg aggccggcct
     5161 gcgcgtcgac accttcctga cgatgtgcga gcccgacggc gcgagcaagt
//
LOCUS       DASVLN010000746         3132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_92583, whole genome shotgun sequence.
ACCESSION   DASVLN010000746 DASVLN010000000
VERSION     DASVLN010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92583"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1225)
                     /locus_tag="VF453_17705"
     CDS             complement(143..1225)
                     /locus_tag="VF453_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX7689547.1"
                     /translation="MTTSLFDPVRFGDIALANRIVMAPLTRDRAGPGRTPHALNAEYY
                     AQRASAGLIVTEGTQVGPLGQGYLDTPGIYSPEQLAGWRQVTDAVHARGGRIVAQIWH
                     VGRISHTSLLPPGEQPVSSVAKLANTKTFTPAGFEPVSAPRALSTAEIAGVIDEFRLA
                     ARHAIEAGFDGVEVHGANGYLVEQFLRDSVNDRTDRYGGSIENRARFLVELMTAVCAE
                     IGPGRVGLRLSPVTPANDIGQDSDPTALFTYVVQQLDKLGFAFIEIVEGATGGARDFA
                     PFDFHALRKHWRGGWIVNNGYTRAMAMAAVAEGRADAVAFGKLFIANPDLPRRLREDR
                     PLNTPDQKTFYGGGVEGYTDYPALAD"
     gene            1495..>3132
                     /locus_tag="VF453_17710"
     CDS             1495..>3132
                     /locus_tag="VF453_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7689548.1"
                     /translation="MLSMRNISVRSRFYLVMSLVTISLLVQAGWGMISARSGIQTVTE
                     MFDQADATATQVGALRESMASVRRYQAEMVATAVSNPTGVEPVHQAWKKEIAALNAGG
                     DGLVKAHPGDAALAASVDKQKKLLGEVVEIIDPIAQQLTDAKMDAAAALAYAGQASEK
                     LDAAQANLAAILKSQQAAAEAIRGQISVSSMIASTARLVLVGITLAIFLPLMWMSLRS
                     ICGPLDQAVAVATRVASGDLVGRIDTEGQDEPARLMKALAEMQASLKSLVGQVRESSE
                     SIEVASSEVASGNTDLSQRTELAASHLQSTASQMEDLTHTVRQSADSAHTANQLASSA
                     AEVAARGGQVVSQVVSTMNDINASSKKISDIIGVIDGIAFQTNILALNAAVEAARAGE
                     QGRGFAVVAGEVRNLAQRSAEAAKEIKSLIGASVERVESGSRLVHDAGTTMTEIVASV
                     QRVTDIIGEISAASTEQSSGIGQVKVAIGEVDQMTQQNAALVEESAAAAESLKEQATK
                     LAAVVSRFKLAEDAAAARAPAPSHDSAADQAIARAKAP"
BASE COUNT          467 a         1084 c         1167 g          414 t
ORIGIN      
        1 cgtgcgcgtg cccgggcgga tcgccgcggg cgagccgttc cagctcgtgc cgggcccgcg
       61 cgaggtcgag ctgcaggcgc tgttccgcgc ccgcgtcggc aaggccgcgc gcgcctgagg
      121 cgcgctggcc cggggccggc cttcagtcgg ccagtgccgg atagtccgtg tagccctcca
      181 cgccgccgcc gtagaaggtc ttctggtcgg gggtgttgag cggccgatcc tcgcgcaggc
      241 ggcgcggcag gtcggggttg gcgatgaaca gcttgccgaa ggccaccgcg tcggcgcggc
      301 cttcggcgac ggcggccatc gccatggcgc gcgtgtagcc gttgttgacg atccagccgc
      361 cgcgccagtg cttgcgcagg gcgtggaagt cgaacggcgc gaagtcgcgc gcgccgccgg
      421 tcgcaccctc gacgatctcg atgaaggcga agccgagctt gtcgagctgc tgcaccacgt
      481 aggtgaacag cgcggtcggg tcggaatcct ggccgatgtc gttggccggc gtgaccggcg
      541 acaggcgcag gccgacgcgg ccggggccga tctcggcgca gacggcggtc atcagctcga
      601 cgaggaagcg ggcgcggttc tcgatcgagc cgccgtagcg gtcggtgcgg tcgttgaccg
      661 agtcgcgcag gaactgctcg accaggtagc cgttggcgcc gtgcacctcc acgccgtcga
      721 agccggcctc gatcgcgtgg cgggccgcca ggcggaactc gtcgatcacg ccggcgatct
      781 ccgccgtcga aagggcgcgc ggcgcggaca ccggctcgaa gccggcgggg gtgaaggtct
      841 tggtgttggc cagcttcgcc acggacgaga ccggctgttc gcccggcggc agcagcgagg
      901 tgtgcgagat gcggccgaca tgccagatct gcgcgacgat ccggccgccg cgcgcatgca
      961 cggcgtcggt cacctggcgc cagcccgcga gttgctcggg gctgtagatg cccggggtgt
     1021 cgaggtagcc ctggccgagc gggccgacct gcgtgccctc ggtcacgatg aggcccgcgc
     1081 tcgcgcgctg ggcgtagtac tcggcgttga gggcatgcgg cgtgcggccg gggccggcac
     1141 ggtcgcgcgt caggggcgcc atgacgatgc ggttggcgag cgcgatgtcg ccgaagcgga
     1201 cggggtcgaa gagggaggtg gtcatgggtc gcgaggttag cggcgctgct tgacggccgc
     1261 cggcgcaggg tcttgcgacg ccgcctggcc gagcgcgggc acccggcgcg gccgttcacg
     1321 cgatcgggtc gcggcccgtg aatttggccg ccaaatccgc ggttttccgc gcttgtgggg
     1381 agttcaagac tcccacaatc gcgccgaaga ccctgaagac gagctcccgc gccagacggg
     1441 agccgggaga cttccccgct gccctcccca cccacgttcc gacgaggcgt ccccatgctg
     1501 tcgatgcgca atatctccgt ccgatcccgg ttctacctgg tcatgtcgct ggtcacgatc
     1561 tcgctgctcg tgcaggcggg atggggcatg atctccgccc gctccggcat ccagacggtg
     1621 acggagatgt tcgaccaggc cgacgccacg gcgacgcagg tcggcgcgct gcgcgagtcg
     1681 atggccagcg tgcgccgcta ccaggccgag atggtcgcga cggccgtctc caacccgacc
     1741 ggcgtcgagc ccgtgcacca ggcctggaag aaggagatcg ccgcgctgaa cgcgggcggc
     1801 gacggcctcg tgaaggcgca cccgggcgac gccgcgctcg ccgccagcgt cgacaagcag
     1861 aagaagctgc tcggcgaggt cgtggagatc atcgacccga tcgcgcagca gctcaccgac
     1921 gccaagatgg atgccgccgc cgccctcgcc tacgccggcc aggccagcga gaagctggac
     1981 gccgcccagg ccaacctcgc cgcgatcctg aagtcgcagc aggccgccgc cgaggcgatc
     2041 cgcggccaga tcagcgtgag ctcgatgatc gcctcgaccg cgcgcctcgt gctggtcggc
     2101 atcacgctgg cgatcttcct gccgctgatg tggatgtcgc tgcgttcgat ctgcgggccg
     2161 ctcgaccagg ccgtcgccgt ggccacgcgc gtggccagcg gcgacctcgt cggccgcatc
     2221 gacaccgagg gccaggacga acccgcgcgc ctgatgaagg cgctcgccga gatgcaggcg
     2281 tcgctcaaga gcctggtcgg ccaggtgcgc gaatcgtcgg agagcatcga ggtcgcgagc
     2341 tccgaggtcg cgagcggcaa cacggacctc tcgcagcgca cggagctcgc cgcgagccac
     2401 ctgcagagca ccgccagcca gatggaggat ctcacccaca ccgtgcgcca gtcggccgac
     2461 tcggcgcaca cggccaacca gctcgcgtcg tcggcggccg aggtcgccgc gcgcggcggg
     2521 caggtggtgt cgcaggtcgt ctcgacgatg aacgacatca acgcgagctc caagaagatc
     2581 tccgacatca tcggcgtgat cgacggcatc gcgttccaga ccaacatcct ggcgctgaac
     2641 gccgccgtgg aagcggcccg tgcgggcgag cagggccgcg gcttcgcggt cgtcgcgggc
     2701 gaggtgcgca acctggcgca gcgctcggcc gaggccgcca aggagatcaa gtcgctgatc
     2761 ggcgcgtcgg tggagcgcgt cgagtcgggc tcgcgcctcg tgcacgacgc cggcacgacg
     2821 atgaccgaga tcgtcgccag cgtgcagcgc gtgacggaca tcatcggcga gatcagcgcc
     2881 gcatcgaccg agcagagctc gggcatcggg caggtcaagg tggcgatcgg cgaggtcgac
     2941 cagatgacac agcagaacgc cgcgctggtc gaggagagcg ccgcggcggc cgagtcgctc
     3001 aaggagcagg cgaccaagct ggccgccgtc gtctcgcgct tcaagctggc cgaggacgcc
     3061 gccgccgcgc gcgcgccggc cccgtcgcac gacagcgccg ccgaccaggc gatcgcgcgc
     3121 gcgaaggcgc cg
//
LOCUS       DASVLN010000747         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_360634, whole genome shotgun sequence.
ACCESSION   DASVLN010000747 DASVLN010000000
VERSION     DASVLN010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360634"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VF453_17715"
     CDS             <1..890
                     /locus_tag="VF453_17715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX7689549.1"
                     /translation="VGDKPIASPAVRAHAWDLGIDLQYVQGSGPAGRITREDIDAYAQ
                     RGKVGGSAPSARDTRYAQRDDEERIEVIGLRRKIAQKMQDAKRRIPHFAYVEEIDVTE
                     LEALRAKLNQKFGAQRGKLTMLPFIMRAVILAVREYPQMNARYDDEAGVVVRSGAVHL
                     GMATQTEAGLMVPVLAHAETLDLWQCAAEVSRLASASRSGKATRDELTGSTITITSLG
                     ALGGIVTTPVINAPEVAIVGVNRVVERPMIRDGLVVARKMMNLSSSFDHRVVDGIHAA
                     EFVQVIRGYLENPATLFVE"
     gene            899..2302
                     /gene="lpdA"
                     /locus_tag="VF453_17720"
     CDS             899..2302
                     /gene="lpdA"
                     /locus_tag="VF453_17720"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7689550.1"
                     /translation="MDIRNTTLLVIGGGPGGYVAAIRAGQLGIPTVLVEGASLGGTCL
                     NVGCIPSKAIIHAATAFEDATSWAGDSPLGIRVQQPSIDLAKTMKWKDGIVGRLTGGV
                     GALLKKNGVQVVKGWARVVDGKTVDVTAAGESAPSVRVTCEHLLLAAGSDAVELPMMR
                     FGGRVISSTEALALTEVPGRLAVVGAGYIGLELGMAYRKLGSEVFVVEALDRILPAYD
                     EELTKPVLAALRKLGVELHLGKSVQGLNDAGDALRVAGKDGATEVLPADRILVAVGRK
                     PHTAGWGLESLMLDMNGRAVKVDEQCRTSMRNVWAIGDLTGEPMLAHRGMAQGEMVAE
                     IIAGKSRHFAPAAIPAVCFTDPEIVVVGRNAAEAQAAGLEAISAMFPFAANGRAMTLE
                     GTDGFVRVVARKDNHRILGWQAVGKGVSELAAAFGQSIEMGALLEDVGHTIHAHPTLG
                     EAVQEAALRALGHALHI"
     gene            2366..2545
                     /locus_tag="VF453_17725"
     CDS             2366..2545
                     /locus_tag="VF453_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689551.1"
                     /translation="MTTRKAHDPPWDRDNPKPDGAHAHLGPRAKASARRRAAKAGRRY
                     PNLVDNMWAASKGRK"
     gene            complement(2562..>2931)
                     /locus_tag="VF453_17730"
     CDS             complement(2562..>2931)
                     /locus_tag="VF453_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689552.1"
                     /translation="DDNRDAADSACMLLGLLGAETSVAYDGAQGLERIGAWRPDVVLL
                     DLGMPGLDGYEIARRVRAGEGGHPDVTLIALTGWGQQQDRQRTRESGFDHHLVKPVDP
                     QALEAVLGSLPRRPRAAERG"
BASE COUNT          428 a         1057 c         1067 g          379 t
ORIGIN      
        1 cggtgggcga caagcccatc gcctcgcccg ccgtgcgcgc gcacgcctgg gacctcggca
       61 tcgacctgca gtacgtccag ggcagcggcc cggccggccg catcacgcgc gaggacatcg
      121 acgcctacgc ccagcgcggc aaggtcggcg gcagcgcgcc gtccgcgcgc gacacgcgct
      181 atgcgcagcg ggacgacgag gagcgcatcg aggtcatcgg cctgcgccgc aagatcgcgc
      241 agaagatgca ggacgccaag cggcgcatcc cgcatttcgc ctacgtcgag gagatcgacg
      301 tcaccgagct cgaggccctg cgcgcgaagc tgaaccagaa gttcggcgcc cagcgcggca
      361 agctgacgat gctgcccttc atcatgcggg ccgtgatcct cgcggtgcgc gagtacccgc
      421 agatgaacgc ccgctacgac gacgaggccg gcgtcgtcgt gcgctccggc gccgtgcacc
      481 tgggcatggc cacgcagacc gaggccggcc tgatggtgcc ggtgctcgcg cacgccgaga
      541 cgctcgacct gtggcagtgc gcggccgagg tctcgcgcct ggcgagcgcc tcgcgcagcg
      601 gcaaggcgac gcgcgacgag ctcaccggct cgacgatcac gatcacgagc ctgggtgccc
      661 tgggaggtat cgtgacgacg cccgtcatca acgcgccgga agtcgccatc gtcggcgtca
      721 atcgcgtcgt cgagcggccg atgatccgcg acggcctggt cgtggcgcgc aagatgatga
      781 acctgtcgtc ctcgttcgac catcgcgtgg tcgacggcat ccacgccgcg gagttcgtgc
      841 aggtgatccg cgggtacctc gagaacccgg ccacgctgtt cgtcgaatga ggagccccat
      901 ggacatccgc aacaccacgc tgctcgtgat cggcggcggg ccgggcggct acgtcgccgc
      961 gatccgcgcc gggcagctgg gcatccccac ggtgctggtc gaaggcgcga gcctcggcgg
     1021 cacctgcctg aacgtcggct gcatcccttc gaaggcgatc atccacgccg ccaccgcctt
     1081 cgaggacgcg acctcgtggg ccggcgactc gccgctgggc atccgcgtgc agcagccgtc
     1141 gatcgacctc gcgaagacga tgaagtggaa ggacggcatc gtcggcaggc tgaccggcgg
     1201 cgtcggcgcg ctgctcaaga agaacggcgt gcaggtcgtc aagggctggg cgcgcgtcgt
     1261 cgacggcaag acggtggacg tcactgccgc cggcgagtcg gcgccgtccg tccgcgtgac
     1321 ctgcgagcac ctgctgctgg ccgccggctc cgacgccgtc gagctgccga tgatgcgctt
     1381 cggcggtcgc gtgatctcgt cgacggaggc gctcgcgctg accgaggtgc ccgggcggct
     1441 cgccgtcgtc ggcgccggct acatcggcct cgagctgggc atggcctacc gcaagctggg
     1501 ctccgaggtc ttcgtcgtcg aggcgctgga tcgcatcctg ccggcctacg acgaggagct
     1561 gacgaagccc gtgctcgcgg cgctccgcaa gctgggcgtc gagctgcacc tgggcaagtc
     1621 ggtgcagggc ctcaacgacg ccggcgacgc gctgcgggtg gccggcaagg acggcgcgac
     1681 cgaggtgctt ccggccgaca ggatcctcgt cgcggtcggc cgcaagccgc acaccgccgg
     1741 ctggggcctc gagtcgctga tgctggacat gaacggccgc gccgtgaagg tcgacgaaca
     1801 gtgccgcacc tccatgcgca acgtctgggc gatcggcgac ctcaccggcg agccgatgct
     1861 ggcgcaccgc ggcatggcgc agggcgagat ggtcgccgag atcatcgccg gcaagtcgcg
     1921 ccacttcgcg cccgccgcga tccccgcggt ttgcttcacc gacccggaga tcgtcgtcgt
     1981 cggccggaac gcggccgagg cccaggccgc cgggctcgag gcgatcagcg cgatgttccc
     2041 gttcgccgcc aacggccgcg cgatgacgct cgagggcacc gacggcttcg tgcgcgtggt
     2101 cgcccgcaag gacaaccacc gcatcctggg ctggcaggcg gtcggcaagg gcgtctccga
     2161 gctcgcggcg gcgttcgggc agtcgatcga gatgggcgcg ctgctggagg acgtgggcca
     2221 cacgatccac gcccatccga cgctgggcga ggccgtgcag gaggccgcgc tgcgggcact
     2281 ggggcacgcc ctgcacatct gaggcccagc acgcggcggg gaacgtcgat tgcaggcccg
     2341 gggcatccga ccgctggagg cccgcatgac gacacgcaag gcacacgacc ctccctggga
     2401 tcgcgacaac ccgaagcccg acggcgcgca tgcgcacctc gggccgcggg ccaaggcctc
     2461 ggcccggcgc cgcgccgcga aggcggggcg gcgctatccc aacctggtcg acaacatgtg
     2521 ggccgcgtcc aagggcagga agtgaccggc ggctgcggcc gtcagccccg ctcggccgcc
     2581 cgcggcctcc ggggcaggct gcccagcacc gcctccagcg cctgcgggtc gaccggcttg
     2641 acgagatggt ggtcgaagcc gctctcgcgg gtgcgctggc gatcctgctg ctggccccag
     2701 ccggtcagcg cgatcagcgt gacgtccgga tggccgccct ccccggcgcg cacccggcgg
     2761 gcgatctcgt agccgtccag gccgggcatg ccgaggtcga gcagcacgac gtcggggcgc
     2821 caggcgccga tccgctccag gccttgcgcg ccgtcgtagg ccacgctcgt ctccgcgccg
     2881 agcaggccca gcagcatgca ggcgctgtcg gcggcgtcgc ggttgtcgtc g
//
LOCUS       DASVLN010000748         8454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_360671, whole genome shotgun sequence.
ACCESSION   DASVLN010000748 DASVLN010000000
VERSION     DASVLN010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360671"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(505..1266)
                     /locus_tag="VF453_17735"
     CDS             complement(505..1266)
                     /locus_tag="VF453_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7689553.1"
                     /translation="MFITGASSGLGQAMALEWARRGGRLALVARRGDEMRAWVAAQGW
                     PAGRAQVYVADARDIPAMTAAGRACIAAQGLPDVVVANAGISVGVDTAIEADLEVIRA
                     TLETNNLGMAATFQPFVAPMCARGSGRLVGIASVAGIRGLPGHAAYSMSKAGAIAYCE
                     SLRGECRPSGVRVVTIAPGYVDTPLTQGNRYSMPFLMPAEKFAAKALDAIAAGVTFRV
                     IPWQMGWVARLLRILPDALYDRVLAGRPRKRRQGE"
     gene            complement(1296..2063)
                     /gene="lptC"
                     /locus_tag="VF453_17740"
     CDS             complement(1296..2063)
                     /gene="lptC"
                     /locus_tag="VF453_17740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX7689554.1"
                     /translation="MKGELHLPDLPEVPVALSPSGGPPEGHERRARQRWHVRVREKVT
                     GYLPLLLMTALALGTWLVAKNTPGLISPVAPGAVRHEPDYTVDRFTLQRFAPDGALKV
                     EIEGDRMSHFPDDDTMEVDRIRVVSTEPDGRRMTATALKGRAKGDGTVVWLDGQAQVV
                     SEMAGEPPIRMNGEHLRAEPKLRRVDSDWPVIVQQAGSEFHADGLFYDADTRVLTLRG
                     KAHALLQPALKQGLSAHDAGGKAGRPSGSKPDAKKPT"
     gene            complement(2060..2647)
                     /locus_tag="VF453_17745"
     CDS             complement(2060..2647)
                     /locus_tag="VF453_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX7689555.1"
                     /translation="MAAPTPVPHLRFPPELLLRAQGPGTGIRAAVFDVDGVLTDGSIW
                     VSAEGESIKPFHVQDGQGLKLLAQGGIAPIVVTGRDSAAVRRRLADLGLPHASCGVAD
                     KRACVEGWLAQLGLGWDELAVIGDDWPDLPLFARAGFACAPADAHPEVIAVAHHVTRR
                     EGGRGAAREFCDLLLTAAGRYAGLLAAMTTTLDAR"
     gene            complement(2647..3642)
                     /locus_tag="VF453_17750"
     CDS             complement(2647..3642)
                     /locus_tag="VF453_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX7689556.1"
                     /translation="MTDLNNDLPFDADRALALARQTFAIEADALLGLQRRLTPEFARA
                     VEAILRCRHRVIVMGMGKSGHVGRKVAATLASTGTPSFFVHPAEAAHGDLGMITQGDV
                     VLAISNSGESDEINAVIPAMRRLEVTLIALTGRPESSLAGVADIVLSCAVDKEACTLN
                     LAPTASTTAQMAMGDALAVALLDARGFREDDFALSHPGGALGRKLLIHVCDLMRTGDA
                     VPAVAPDASFSTLLKEMSSKGLGLAAVVEGDTPIGIFTDGDLRRLIETGDDLRGHVAR
                     EVMRPKPRTIRSDALAVDAASEMEHHRITSLLVVDAQGRLVGAVNTYDLLHAKVI"
     gene            3685..5682
                     /locus_tag="VF453_17755"
     CDS             3685..5682
                     /locus_tag="VF453_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7689557.1"
                     /translation="MASTLELALLYLAAAIAGVVAFRMLRLPPVLGYLVAGVLIGPNA
                     LELAHDTAGVRNLAEFGVVFLMFVIGLEFNLPKLRAMRTLVFGLGASQVALTIVGAIA
                     GNFALVWAFKLLGRPWDLDWRGAAVLGSAFAMSSTALVVKLMAERLELESEHGRRVMG
                     VLLFQDLAVVPLLVLIPSLGQKAGELVATLVPALLKAAVVLAVLLSGGQKVMRWFLTV
                     ISRRKSEELFMLSLLGVTLGMAWLTEEAGLSLALGAFVAGMLIAETEYKHQVETDIRP
                     FHDVLLGLFFITIGMKLDWRTLADEWALVVLLTTLPILAKAALVAGLARVFGAAPGVA
                     LRTGLYLAQAGEFGFVLLTLGTDSHALAERWIAPVLASMVVSMLAAPFLVLQSNRIVM
                     KLASSDWLMQSVALTTIARRAIQSEKHVIICGYGRSGQNLARMLEVERIPYMALDLDP
                     DRVRQAAAAGQNVVFGDAARLSSLMAAGLARSAAVVVSYHDTPSALKVLHLVQTHAPR
                     VPVLVRTIDDTDLEVLRAAGATEVVPEAIEGSLMLASHALALVGVPMRRVLRLSRDFR
                     DARYGLLRGFFHGADDDAAGDQQQARLASVTLPPDAGCAGRTLAEATTGIEGVHVVSL
                     RLASGRVVDARPEQLLASGDTLVLAGMPEPLRLAEEKLTTA"
     gene            complement(5897..6370)
                     /locus_tag="VF453_17760"
     CDS             complement(5897..6370)
                     /locus_tag="VF453_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7689558.1"
                     /translation="MTTNISNLVQAITTLNAYDAFRPRFSPENWRVVGSYLIRREMKS
                     GDVLIYQGDSDRTMYFIESGTLQVYVRGTATSAAKIALLRAGAIVGEPALFGETVRMA
                     QVDSISPSIVWALSRPRLDEMLAGQPELAYEILRAAGAVMAERMRGTLERGTPVA"
     gene            complement(6580..6882)
                     /gene="fliO"
                     /locus_tag="VF453_17765"
     CDS             complement(6580..6882)
                     /gene="fliO"
                     /locus_tag="VF453_17765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX7689559.1"
                     /translation="MENFATSVATMLVALAFVLLLAWGALHLLKRFQLGRGGAAAGAP
                     QALRFVRALPVGARERVVVVHYRGEEWVLGVTAGGISLLAREPVPAPMPDEAQASK"
     gene            complement(6884..8068)
                     /gene="sctU"
                     /locus_tag="VF453_17770"
     CDS             complement(6884..8068)
                     /gene="sctU"
                     /locus_tag="VF453_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctU"
                     /protein_id="HEX7689560.1"
                     /translation="MADKNDGGDKTEQPTPRKLRDARKEGNVWKSREATSTLVLLVWL
                     VLGAVAAGLVGLRLQALVQASLQSLDGDFATRVRDLGWLAVEGLVLVTASLLVPAMLA
                     GALVEFLQVGPVLAFTKLKPSFDKLNPAEGLKRMFSADNLVEAAKSLLKTAVLAVVGW
                     VVLRGSLAQFAALAGEDPHLVAPRLEQLLWQSSVRLAGATVAAFAGLAILDAAWQRHS
                     FMKKMRMSRRDIRDEHKQDEGDPHVKAQRRQTHREWSERNAAQAARQADVLVVNPTHV
                     AIALDYDHEGQGVPTVSAKGEDEVARAMREAAEEAGVPIVRNVALARELLATTEVGAV
                     VPRHLFDIVAEVILWAREVREEVERQRRGQPAPAAARQRLARAPGEDATRYPGQPSPS
                     LD"
BASE COUNT         1129 a         3013 c         3104 g         1208 t
ORIGIN      
        1 ggcggtggtg gcggcggcgg ccgttccggc ggcggcggct acggcggtgg tggcggcggc
       61 tacggcggcg gtggccgttc cggtggcggc ggctacggcg gcggcggccg ctccggtggc
      121 ggcggtggcg gcggctactg atcctcgcca cacggcctga ccgacacgca tccgtcgatc
      181 cgtcgacgag aaacaagccc tggcgcctcg cggcgcccgg gcttttctct tttcagcggc
      241 cgcgaccgag cgcatcggtc ggacgtctgg cgagcgtgac cgtgctgcgg cagcggcgcc
      301 gagagcctcg ccgcgccgcg aattcctcgt ccaacgcgat cgagcgcctc ggagcgcgcc
      361 gaaacgaggt caatcccacg attgaaggga caccgcgacc gggacgcacg agagcgcggc
      421 gggatggccg acgcccgcat cgcgcgggca gtcgtcggct gcccgcgcag ggcggatccg
      481 tggccgcagg cgccgccgca cgcgctattc gccctggcgt cgcttgcgcg gccgcccggc
      541 cagcacgcgg tcgtagagcg cgtcgggcaa tatccgcagc agccgcgcga cccagcccat
      601 ctgccaggga atcacgcgga aggtgacgcc ggcggcaatc gcgtccagcg ccttggcggc
      661 gaatttctcg gccggcatca ggaagggcat cgaatatcgg ttgccctgcg tgagcggagt
      721 atcgacatac cccggcgcga tggtgacgac gcgcacgccc gacggccggc attccccgcg
      781 caggctctcg cagtaagcga tcgctccggc cttgctcatg ctgtacgcgg catgtcccgg
      841 cagcccgcgg atgccggcga cgctggcgat cccgaccagc cggccgctgc cacgcgcgca
      901 catcggcgcc acgaagggct ggaaggtcgc ggccatgccg aggttgttgg tctcgagcgt
      961 ggcgcggatc acctcgaggt cggcctcgat cgccgtgtcg acgcccacgc tgatgccggc
     1021 gttcgccacg acgacgtcgg gcagcccttg cgccgcgatg caggcgcggc ccgcggcggt
     1081 catcgcgggg atgtcgcggg cgtcggcgac gtagacctgc gcgcggcccg cgggccagcc
     1141 ctgcgcggcc acccaggcgc gcatctcgtc gccgcgccgc gcgaccagcg cgagccgccc
     1201 gccccgacgc gcccactcca gcgccatcgc ctgtcccagg ccgctggaag cgccggtgat
     1261 gaacatcagg ggggcggtct tcggggcggt ggcgttcacg tgggcttctt cgcgtcgggc
     1321 ttcgatccgg agggccgacc ggccttgcca ccggcgtcgt gcgccgacag gccctgcttc
     1381 agcgccggtt gaagcagggc gtgggccttg ccgcgcaggg tcagcacgcg cgtgtcggca
     1441 tcgtagaaca ggccgtcggc gtggaactcg ctgccggcct gctgcacgat gaccggccag
     1501 tcggagtcga cgcgccgcag cttcggctcg gcgcgcaggt gctcgccgtt catgcggatc
     1561 ggcggctcgc cggccatctc gctgacgacc tgggcctggc cgtcgagcca gaccaccgtg
     1621 ccgtcgccct tggcgcggcc cttcagcgcg gtggccgtca tgcggcggcc gtccggctcg
     1681 gtggagacga cgcggatgcg gtcgacctcc atggtgtcgt cgtccgggaa gtggctcatg
     1741 cggtcgccct cgatctccac cttcagcgcg ccgtcggggg cgaagcgctg cagcgtgaag
     1801 cggtcgaccg tgtagtcggg ctcgtggcgg accgccccgg gcgccaccgg cgagatcagc
     1861 cccggcgtgt tcttcgcgac gagccaggtg ccgagcgcga gcgcggtcat cagcagcagg
     1921 ggaaggtagc cggtgacctt ctcgcgcacg cgcacgtgcc agcgctggcg cgcgcgacgc
     1981 tcgtggccct cgggcggccc gcccgacggg ctcagcgcga ccggcacctc cggcaggtcg
     2041 gggaggtgga gctcgccctt catcgcgcgt cgagcgtcgt cgtcatggcc gcgagaagac
     2101 cggcatagcg gccggccgcg gtcagcaaca ggtcgcagaa ctcgcgcgcg gcgccgcggc
     2161 cgccctcgcg ccgcgtcacg tggtgggcga cggcgatcac ctcgggatgg gcgtcggcgg
     2221 gcgcgcaggc aaagccggcg cgggcgaaca ggggcaggtc gggccagtcg tcgccgatga
     2281 cggcgagctc gtcccatcca aggcccagct gcgcgagcca gccctcgacg caggcgcgct
     2341 tgtcggcgac gccgcaggac gcgtgcggca ggccgaggtc ggccagccgc cgccgcacgg
     2401 ccgccgagtc ccgccccgtc accacgatgg gcgcgatgcc gccctgcgcg agcagcttca
     2461 ggccctggcc gtcctgcacg tggaagggct tgatggactc gccctctgcc gagacccaga
     2521 tggagccgtc cgtgagcacg ccgtcgacgt cgaacaccgc tgcgcggatg ccggtgccgg
     2581 ggccctgcgc ccgcagcagc agctcgggcg ggaagcgcag gtgcggcacg ggcgtggggg
     2641 cggccatcag atgaccttcg cgtgcagcag gtcgtaggtg ttgaccgcgc ccaccagccg
     2701 gccctgcgca tcgacgacca gcaggctcgt gatgcggtgg tgctccatct cgctggcggc
     2761 gtcgacggcc agcgcgtcgg agcggatggt gcgcggcttc ggcctcatca cctcgcgcgc
     2821 gacgtggccg cgcaggtcgt cccccgtctc gatcaggcgg cgcaggtcgc cgtcggtgaa
     2881 gatgccgatc ggggtatcgc cctcgaccac cgcggccagc cccagcccct tggacgacat
     2941 ctccttgagc agcgtcgaga agctcgcgtc cggcgccacc gccggcacgg cgtcgccggt
     3001 gcgcatcagg tcgcagacgt ggatcagcag cttgcgcccg agcgcgccgc cggggtgcga
     3061 gagcgcgaag tcgtcctcgc ggaagccgcg cgcgtccagc agcgcgacgg ccagggcgtc
     3121 gcccatggcc atctgcgcgg tcgtgctcgc ggtgggcgcg aggttgaggg tgcaggcttc
     3181 cttgtcgacg gcgcaggaca gcacgatgtc ggcgacgccc gcgagcgagg actcgggccg
     3241 cccggtgagc gcgatcagcg tcacctccag ccgccgcatc gccgggatga cggcgttgat
     3301 ctcgtcggac tcgcccgagt tggagatcgc gagcaccacg tcgccctgcg tgatcatgcc
     3361 gaggtcgccg tgcgcggcct cggccggatg cacgaagaac gacggcgtgc cggtggacgc
     3421 gagcgtcgcg gcgaccttgc gcccgacgtg gccgctcttg cccatgccca tcacgatgac
     3481 gcggtggcgg cagcgcagga tggcctcgac cgcgcgcgcg aactcgggcg tgaggcgccg
     3541 ctgcagcccg agcagggcgt cggcctcgat cgcgaacgtc tggcgcgcaa gggcgagcgc
     3601 gcgatccgcg tcgaacggca ggtcgttgtt caagtcggtc atcggtgttc taggatgggc
     3661 accgattcta gtgtttcgtc ccgcatggca tcgaccctcg aactcgcgct gctctacctc
     3721 gccgccgcca tcgcgggcgt cgtcgcgttc cgcatgttgc ggctgccgcc ggtgctcggc
     3781 tacctcgtcg cgggggtgct gatcggcccc aacgccctgg aactcgccca cgacaccgcc
     3841 ggcgtgcgca acctggcgga gttcggcgtc gtcttcctga tgttcgtgat cggcctggag
     3901 ttcaacctgc ccaagctgcg ggcgatgcgc acgctggtct tcgggctcgg ggcgtcgcag
     3961 gtggcgctga ccatcgtcgg ggcgatcgcg ggcaacttcg cgctggtatg ggcgttcaag
     4021 ctgctcgggc ggccctggga cctggactgg cgcggcgccg ccgtgctggg cagcgcgttc
     4081 gcgatgtcca gcaccgcgct ggtcgtcaag ctgatggccg agcgcctgga gctcgagagc
     4141 gagcacggcc gccgcgtgat gggcgtgctg ctgttccagg acctcgcggt ggtgccgctg
     4201 ctggtgctga tcccgtcgct gggccagaag gcgggcgagc tggtcgcgac gctggtgccg
     4261 gcgctgctga aggcggcggt cgtgctggcg gtgctgctgt cgggcgggca gaaggtgatg
     4321 cgctggttcc tcaccgtcat ctcgcgccgc aagagcgagg agctcttcat gctgagcctg
     4381 ctcggcgtga cgctgggcat ggcgtggctc accgaggagg cggggctgtc gctcgcgctg
     4441 ggcgcgttcg tcgccggcat gctgatcgcc gagaccgagt acaagcacca ggtcgagacc
     4501 gacatccgtc cgttccacga cgtgctgctg ggcctgttct tcatcacgat cggcatgaag
     4561 ctggactggc gcacgctggc cgacgagtgg gcgctcgtgg tgctgctgac cacgctgccc
     4621 atcctcgcca aggcggcgct ggtggccggc ctcgcgcgcg tgttcggcgc cgcgcccggg
     4681 gtggcgcttc gcaccggcct gtatctcgcg caggcgggcg agttcggctt cgtgctgctg
     4741 acgctgggca ccgacagcca cgcgctcgcg gagcgctgga tcgcgccggt gctcgcgagc
     4801 atggtggtgt cgatgctcgc cgcgcccttc ctcgtgctgc agtccaaccg catcgtcatg
     4861 aagctcgcga gcagcgactg gctgatgcag tcggtggcgc tcacgaccat cgcccggcgc
     4921 gcgatccaga gcgagaagca cgtgatcatc tgcggctacg gccgctcggg gcagaacctc
     4981 gcacgcatgc tggaggtcga gcgcatcccg tacatggcgc tcgacctcga ccccgaccgc
     5041 gtgcggcagg ccgccgcggc gggacagaac gtcgtgttcg gcgacgcggc acggctctcg
     5101 agcctgatgg ccgccgggct ggcgcgttcg gcggcggtcg tcgtgagcta ccacgacacg
     5161 ccgtccgcgc tgaaggtgct gcacctcgtc cagacccatg cgccgcgcgt gccggtgctg
     5221 gtgcgcacga tcgacgacac cgacctcgag gtgctgcgcg ccgcgggcgc caccgaggtc
     5281 gtgcccgagg cgatcgaagg ctccctgatg ctggcctcgc acgcgctcgc gctggtgggc
     5341 gtgccgatgc ggcgggtgct gcggctgtcg cgcgacttcc gcgacgcgcg ctacggcctg
     5401 ctgcgcggct tcttccacgg cgcggacgac gatgccgccg gcgaccagca gcaggcgcgg
     5461 ctcgcgagcg tcacgctgcc gcccgacgcc ggctgcgccg ggcgcacgct ggccgaggcg
     5521 acgaccggca tcgagggcgt gcacgtcgtc tcgctgcggc tggcaagcgg ccgcgtcgtc
     5581 gatgcccgac ccgaacagct cctggccagc ggcgacacgc tggtgctcgc gggcatgccg
     5641 gagcccctga ggctcgcaga agaaaagctg acgacggcgt gacgggcccc cacgcccgct
     5701 ggcgcgggcc gccccgcgag gggtgagccc ggacacaggc cgtccagtgg acggaccgtg
     5761 cctggcgaac gccggccgct tgcagccggc aggcatgcag accggcaaag ccggatctgc
     5821 cgcttccccg tgtctcgttt gcgccgctcg agcggcagcg ccggcaaccg tcggcgcatc
     5881 gctgcgcccg cgcttttcaa gccaccgggg tgccgcgttc gagcgtgccg cgcatgcgct
     5941 cggccatcac ggcgccggcg gcgcgcagga tctcgtaggc gagctcgggc tggccggcca
     6001 gcatctcgtc gaggcgcgga cggctcaggg cccagacgat gctgggcgag atcgagtcga
     6061 cctgggccat ccgcacggtc tcgccgaaca gcgccggctc gccgacgatg gcgccggcgc
     6121 gcagcagcgc gatcttggcg gcggacgtgg ccgtgccgcg cacgtagacc tgcagcgtgc
     6181 ccgactcgat gaagtacatc gtgcgatcgg agtcgccctg gtagatcagg acgtcgccgc
     6241 tcttcatctc gcgccggatc aggtacgagc cgaccacgcg ccagttctcc ggcgagaagc
     6301 gcggacggaa ggcgtcgtag gcgttcagcg tcgtgatcgc ctggaccagg tttgaaatgt
     6361 tcgtcgtcac tcggaaattc ccccaacggc gccggctcgc tgtcgagcgg aggcggctgg
     6421 caccgcgccg gcgtcatcgc aggcccttgg cgccgattgt cacgtgcccg tccgcgcccg
     6481 gcaaggcatt gtgtgtcatc cgtcggagcc ggggccccgt ctgtcgtgtg agcgggacgc
     6541 aggctcacgc cagagcgcgg acgcggtgga ggcgtgcgct cacttggaag cctgcgcctc
     6601 gtccggcatc ggcgccggta ccggctcgcg ggcgagcagc gagatcccgc ccgccgtcac
     6661 gcccagcacc cattcctcgc cgcgatagtg gacgacgacg acccgttcgc gtgcgccgac
     6721 cggcagcgcg cggacgaagc gcagggcttg cggcgcaccc gccgcggcgc cgccgcgtcc
     6781 gagctggaag cgcttgagca ggtgcagcgc gccccaggcg agcaggagga cgaaggcgag
     6841 cgcgaccagc atcgtcgcga ccgacgtggc gaagttttcc atgtcaatcg agggagggcg
     6901 atggctggcc ggggtagcgg gtcgcgtcct cgcccggagc gcgcgccagc cgctggcgcg
     6961 ccgccgcggg ggccggctgg ccgcggcgct ggcgctcgac ctcctcgcgc acctcccgcg
     7021 cccagaggat gacctcggcc acgatgtcga acaggtggcg cggcacgacg gcgccgacct
     7081 ccgtggtcgc aagcagctcg cgcgccagcg ccacgttgcg cacgatgggc acgccggcct
     7141 cctcggcggc ctcgcgcatc gcgcgcgcga cctcgtcctc gcccttggcc gagaccgtgg
     7201 gcacgccctg gccctcgtgg tcgtagtcca gcgcgatcgc gacgtgggtc gggttgacga
     7261 ccagcacgtc ggcctggcgc gccgcctggg cggcattgcg ctccgaccat tcccggtgcg
     7321 tctggcgacg ctgcgccttc acgtgcggat cgccctcgtc ctgcttgtgc tcgtcgcgga
     7381 tgtcgcgccg gctcatgcgc atcttcttca tgaacgagtg gcgctgccag gcagcgtcga
     7441 ggatcgcgag gcccgcgaac gccgcgacgg tcgccccggc gaggcggacg ctggactgcc
     7501 acagcaactg ctccagccgc ggcgcgacga gatgcggatc ctctcccgcg agcgccgcga
     7561 actgcgccag cgagccgcgc agcaccaccc agccgacgac ggcgaggacg gccgtcttca
     7621 gcagcgactt cgccgcctcg accaggttgt cggccgagaa catgcgcttg aggccctcgg
     7681 cgggattgag cttgtcgaac gagggcttca gcttcgtgaa cgccagcacc gggccgacct
     7741 gcaggaactc gaccagcgcg cccgccagca tcgcgggcac cagcagcgag gcggtcacga
     7801 gcacgaggcc ctcgacggcc agccagccga ggtcgcgcac gcgcgtggcg aaatcgccgt
     7861 ccagcgactg cagcgacgcc tgcacgagcg cctgcagccg cagcccgacg aggcctgcgg
     7921 cgacggcgcc cagcacgagc cagacgagca gcaccagggt gctcgtcgcc tcgcggctct
     7981 tccagacgtt gccttccttg cgggcgtcac gcagcttgcg cggcgtgggc tgttcggtct
     8041 tgtccccgcc gtcgttcttg tcggccatcg cgggatcttc gccggtcgcc cgcgacattt
     8101 cgatggccgc gcaagccaat tgttgccaaa tattacctag gggggtatgc ccgacagtgg
     8161 gctacgaaaa cccgtacccc ttaatgaagg caagcgtgcc cgtttccgca gcgcgggaat
     8221 atttcacgga tacgcaaggt tacagagtaa caagatccgt cacccatcgg acatcggacg
     8281 cggcaaagat tcgcccgccc cggccaacgc aagctcgggg tggcggccgg aagcggtcga
     8341 cccagggagc gtacaaaaat gaaactcgcc cacgtcatcg tgggcctgtg cctggccggc
     8401 ggcctgctgt cggcccgggc ggatgtcgcg tcggtctgcg cggcggccgc cccc
//
LOCUS       DASVLN010000749         9112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_92664, whole genome shotgun sequence.
ACCESSION   DASVLN010000749 DASVLN010000000
VERSION     DASVLN010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92664"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VF453_17775"
     CDS             complement(<1..532)
                     /locus_tag="VF453_17775"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX7689561.1"
                     /translation="MDGLRRGAGGPGRPAASAAPLLRYGMGQPAPRRTPAARARPPRT
                     DGAETRRRILQVAGELCAERGFAGMTSKEICERAQVNVAAVNYHFGSRDALYDAVLVE
                     AHRQLISMEELARITTMPAAPQDRLQALLRALVARAGEPPTWGARVLARELMSPSGRS
                     TGLLRDAVLPKSRLLYA"
     gene            635..1846
                     /locus_tag="VF453_17780"
     CDS             635..1846
                     /locus_tag="VF453_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7689562.1"
                     /translation="MSTIRKTQCCIAGGGPAGMMLGLLLARAGIEVVVLEKHADFLRD
                     FRGDTVHPSTLTVMDELGLLDRFLRLPHQRMERAEGLFGTERVRVADFARLPVKCPYI
                     AFMPQWNFLDFLAGEARRLPNFTLLMQAEAHALRREGDRIAGVVARTPQGELAIDAVL
                     TVGADGRGSAIRTAAGLRPRALGAAMDVLWFRVARDPSDADNALLRVRDGHVLVTIDR
                     GDYWQCAYVIPKGGADAVRARGLAALHDDVAAVDPALRAALAEVRSWDDVKLLTVTVD
                     RLDRWHLPGLLFIGDAAHAMSPIGGVGINLAVQDAVAAHNLLAPALRRGDVAEDVLAA
                     VQRRRRLPTALTQRVQMLAQDALIRPALQGRSVRHAPLALRVVSHSRLLQGLMARLIG
                     TGIRPEHVAAP"
     gene            1941..3305
                     /gene="radA"
                     /locus_tag="VF453_17785"
     CDS             1941..3305
                     /gene="radA"
                     /locus_tag="VF453_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX7689563.1"
                     /translation="MAAPKTVYTCTACGGTNPKWLGRCPHCSAWNTLEEGIAEPGAGG
                     GRNRFQGLARSQPVTTLSEIDAADVARTPTGIEELDRVLGGGIVAGGVVLIGGDPGIG
                     KSTLLLQAVDALATQVKVLYVTGEESGAQLALRSRRLDLSGAHVRVLAEINLERILAT
                     IEAEQPAFCVIDSIQTLYSEQLASAPGSVAQVRECSAQLTRTAKAGGCAMVLVGHVTK
                     DGALAGPRVLEHIVDTVLYFEGDTHSSFRLVRAFKNRFGAVNEIGVFAMTERGLKGVA
                     NPSAIFLSTHGEPVPGSCVLVTLEGTRPMLVEIQALVDTGGPSPRRLSVGLERDRLAM
                     LLAVLHRHAGVSTGDQDVFVNAVGGVRISEPAADLAVLLAIQSSLRNRALPRGFLAFG
                     EVGLAGEVRPAPRGQDRLKEAAKLGFSVAIVPKANAPRKPIEGLTIHAVERIEEAIDV
                     LRGL"
     gene            complement(3526..3948)
                     /locus_tag="VF453_17790"
     CDS             complement(3526..3948)
                     /locus_tag="VF453_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009935592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEX7689564.1"
                     /translation="MTDRLPPERRSWLMSRVRSSDTRPEMTVRSALHALGFRYRLHQK
                     DLPGTPDLVFRSRNKVVFVHGCFWHGHHCRLGQAASKSNVEFWADKIRRNVARDRRNI
                     TALRRQGWGVAVVWECELKKRMGDKWILRIIRFLERQA"
     gene            4043..5161
                     /locus_tag="VF453_17795"
     CDS             4043..5161
                     /locus_tag="VF453_17795"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEX7689565.1"
                     /translation="MTQQNKKVIDLFAGVGGLSLGAARAGFDVAAAVEFDAKAAAQHA
                     VNFPRSNHLVEDVAALSGERLLNLSGVASNDLCGLIGGPPCQGFSEIGLKGDGDPRNE
                     LLVHFFRLVAELSPAFFLAENVPGVTHERNKPIVERALSLVPKNYVTLPPMTVKANDY
                     GAPTTRTRVFFCGYDPNRVSALTVASFAPKVGQDVRVSHALSGLPIIRSDWQTEAQSW
                     RAVRKLDKGAYEHRISNHIPFGVGDKDAIQAYSNHSRVSGFLGTLHTPETKKRFKVLQ
                     PGMVDAISRSVRLRMDGYCPTLRAGTGPERGSYQAIRPIHPSSPRVIAPREAARLQGF
                     PDWFQFHPTKWHAFRQIGNSVSPIVSEALLTTIYEAVS"
     gene            5319..6581
                     /locus_tag="VF453_17800"
     CDS             5319..6581
                     /locus_tag="VF453_17800"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689566.1"
                     /translation="MITRDILLRDCIFDLLDNSIDGARRKPSNNPDKPFEGHLIELTF
                     SEENFKIADNCGGIRLSDAIDYAFHFGKKANSPTDVSGGIGLYGIGMKRAIFKIGRIC
                     VVTSHADDASFKVSIDVDEWESRTDWDFDYEDIERSDARGTTVNISRLNDGAKEALGD
                     AVFKNDLMRAIARDYAFFIQQGLKIQVGGVDVPSYKFQLRSSDSLAPSVEHYVDNGVD
                     VRILAGLVDELPDDIPDELKPDKVERYGWFVICNDRVVLAANKADETIWGDDDFNVWH
                     PQYNGFAGYVIFQAADQRKLPWTTTKRELDASSPLYRRTIARMKEVTRLFIQYTNARK
                     ADLVTAKAAETPRAQVNISELREVQPLRLPAISAAATRPDMVNITYRREKREVEEIKR
                     HLGSIAMSARDVGIKTFEYYREVELGKE"
     gene            6578..7615
                     /locus_tag="VF453_17805"
     CDS             6578..7615
                     /locus_tag="VF453_17805"
                     /inference="COORDINATES: protein motif:HMM:NF042830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX7689567.1"
                     /translation="MSRNTPSFERIDYLLRTNKHIERKLVFDILDGVAQQLDFKTHWY
                     LGFGSMWFGDFKLAHRRLGIDKMISMERKDHASRAKFNRPFAGVNVQPGESSTIIAGL
                     PADAWDKPLVAWLDYDGYLNEDVVNDIELLLSRARPNSAIIVTVNGARGTYRVKKQSG
                     VLTREETSVGVVETFTGKGSVSEKYDPKPNGAGNYIDVKEAEFPEFLTEAISVFMQHR
                     VVSLAREVDGQRLKFVPLYRLHHCDGADMVTVGGALVTEEGEAAWRDCVARHPLLTDD
                     VGEVAYCRLDLVPVTVKEKIALDECLPDQGNGAQFLEAAKAVGVALSDEEMTKYRKFY
                     RHFPVFVETTM"
     gene            complement(7830..>9112)
                     /locus_tag="VF453_17810"
     CDS             complement(7830..>9112)
                     /locus_tag="VF453_17810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX7689568.1"
                     /translation="GWPCSHSGTLVGELRTPLPAGLARDDWGDRLAPALDTAAALLAG
                     LIEHAGHALPGSLALIRTAIHEAGTFVWEWDIASDVLGDIDEGERMLGYAPNTLGHTQ
                     ADWDRIVHPEDQPAIARAYAAHERGETAMYRVVYRALAADGLWRWIEERGRIVEWDRH
                     GRPRRMFGTQTDATLLRDLEQAQRDRLAAEAANSAKTLFLSRISHELRTPLNAVLGFA
                     QLLSTDPALDGLPTQRQQVRMIRDAGEHLLAMIGDLLDLSLAEAAHLPIQLQNVALAP
                     LFEACAAWCRPQADAARIALRVEDPDAGASPLAPVARVRADPTRLRQVITNLLSNAVK
                     YNRAGGRVTLRAIAQPEEWRIEVEDTGHGLTPAERARLFQPFNRLGREHSGVAGAGLG
                     LALSQSLAQLMGGRITVQSVAGAGSTFALALPAA"
BASE COUNT         1607 a         2935 c         3040 g         1530 t
ORIGIN      
        1 gcgcgtacag cagccgggac ttgggcagca ccgcgtcgcg cagcaggccg gtgctccggc
       61 ccgacggcga catcagctcg cgcgcgagca cgcgcgcgcc ccaggtcggc ggttcgccgg
      121 cccgcgcgac gagggcccgc agcagcgcct gcagcctgtc ctggggcgcc gccggcatcg
      181 tcgtgatgcg cgcgagctcc tccatcgaga tgagctggcg atgggcctcg accagcaccg
      241 cgtcgtacag cgcgtcgcgg ctgccgaagt ggtagttcac cgcggccacg ttgacctgcg
      301 cgcgttcgca gatctccttg ctcgtcatgc cggcgaagcc gcgctccgcg caaagctcgc
      361 cggcgacctg caggatgcgc cgccgcgtct cggcgccgtc cgtgcgcggc ggccgcgcgc
      421 gggcggcggg tgttcgtctc ggtgctggct ggcccatgcc gtagcgtagc agcggcgcgg
      481 ccgacgccgc cgggcggccg gggccgcctg cgccacgacg caggccgtcc attcccgtgg
      541 catcattcgg tcaaattcaa atttgattta ggcacgcgag ttgccgcgcc gctggcgaat
      601 ccgcgatcca tccaccgatc cacaggagcc gatgatgtcg accatccgaa agacgcagtg
      661 ctgcattgcc ggaggcggcc cggcggggat gatgctgggc ctgctgctcg cgcgcgccgg
      721 catcgaggtc gtcgtgctcg agaagcacgc cgacttcctg cgcgacttcc gtggcgacac
      781 cgtgcatccc tcgacgctga cggtgatgga cgagctcggg ctgctggatc ggttcctgcg
      841 gctgccgcat cagcggatgg agcgcgcgga gggcctgttc ggcaccgagc gcgtgcgggt
      901 ggcggacttc gcccgcctgc cggtgaagtg cccctacatc gccttcatgc cgcagtggaa
      961 cttcctggac ttcctggccg gcgaggcgcg gcggctgccc aacttcacgc tgctgatgca
     1021 ggccgaggcg cacgcgctgc ggcgcgaggg agaccggatc gcgggcgtcg tcgcgcgaac
     1081 gccgcagggc gagctcgcca tcgatgccgt tctcacggtg ggcgccgacg gccggggctc
     1141 ggcgatccgc accgccgccg ggctgcggcc gcgcgcgctc ggcgcggcga tggacgtcct
     1201 ctggttccgc gtcgcccgcg acccctcgga cgccgacaac gcgctgctgc gggtgcggga
     1261 cggccatgtc ctcgtgacga tagaccgcgg cgactactgg caatgcgcct acgtgatccc
     1321 caagggcggc gccgacgccg tccgcgcgcg cggcctggcc gcgctgcacg acgacgtcgc
     1381 cgccgtcgat cccgccctgc gcgcggccct ggcggaggtg cgcagctggg acgacgtgaa
     1441 gctgctgacg gtgaccgtcg accgcctcga ccgctggcac cttccgggcc tgctgttcat
     1501 cggcgacgcc gcgcacgcga tgtcgccgat cggcggcgtc ggcatcaacc tggcggtgca
     1561 ggacgcggtc gctgcgcaca acctgctcgc ccccgcgctg cggcggggag acgtcgccga
     1621 ggacgtcctc gccgccgtgc agcgcaggcg ccggctgccc acggcgctca cccagcgcgt
     1681 gcagatgctg gcgcaggacg cgttgatccg gcccgcgctg cagggccggt cggtgcggca
     1741 tgcgccgctc gcgctgcggg tcgtctcgca cagccggctg ctccagggcc tgatggcgcg
     1801 cctgatcggc accggcattc gcccggagca cgtggcggca ccatgacagg ccgcggccga
     1861 ccggcgcgcc cgtgtcgaat cgggcattca tcggtcgttt cggcaggctg tggttttgaa
     1921 cagtaggtga caatgggcgc atggccgcac ccaagaccgt ctacacctgc accgcctgcg
     1981 gcggaacgaa tcccaagtgg ctgggccgct gcccgcactg cagcgcctgg aacacgctgg
     2041 aggaaggcat cgccgagccc ggcgccggcg gcggcaggaa ccgcttccag ggcctcgcac
     2101 gcagccagcc cgtgacgacc ctgtccgaga tcgacgccgc cgacgtcgcg cgcacgccca
     2161 cgggcatcga ggagctcgac cgcgtcctcg gcggcggcat cgtcgccggc ggcgtggtgc
     2221 tgatcggggg cgacccgggc atcggcaaga gcacgctgct gctgcaggcc gtggacgccc
     2281 tggccacgca ggtgaaggtg ctctacgtca ccggcgagga atccggcgcc cagctcgcgc
     2341 tgcgctcgcg ccggctcgac ctctccggcg cccacgtgcg cgtcctggcc gagatcaacc
     2401 tcgagcgcat cctcgccacc atcgaggcgg aacaacccgc cttctgcgtg atcgactcga
     2461 tccagacgct ctactcggag cagctcgcct cggcgcccgg ctcggtggcg caggtgcgcg
     2521 aatgctcggc gcagctcacg cgcacggcca aggcgggcgg ctgcgcgatg gtgctggtgg
     2581 ggcacgtgac caaggacggc gcgctcgcgg gcccgcgcgt gctggagcac atcgtcgaca
     2641 cggtgctgta cttcgagggc gacacgcact ccagcttccg cctcgtgcgc gcgttcaaga
     2701 accgcttcgg cgcggtcaac gagatcggcg tgttcgcgat gaccgagcgc ggcctgaagg
     2761 gcgtcgccaa cccgagcgcg atcttcctgt ccacgcacgg cgagccggtg cccggctcct
     2821 gcgtgctcgt gacgctggag ggcacgcggc cgatgctggt cgagatccag gccctcgtcg
     2881 acaccggcgg cccgagcccg cgccggctgt cggtcggcct cgagcgcgac cgcctcgcga
     2941 tgctgctggc ggtgctgcac cggcacgcgg gcgtgtccac cggcgaccag gacgtgttcg
     3001 tcaacgcggt cggcggcgtg cgcatcagcg agccggcggc cgacctggcg gtgctgctgg
     3061 cgatccagag ctcgctgcgc aaccgcgcgc tgccgcgcgg cttcctggcc ttcggcgagg
     3121 tcggcctcgc gggcgaggtc cgtcctgcgc cgcgcggcca ggatcgcctg aaggaggccg
     3181 ccaagctcgg cttctcggtg gcgatcgtgc ccaaggccaa cgcccccagg aagccgatcg
     3241 agggcctcac gatccatgcg gtggagcgga tcgaggaggc gatcgacgtg ctgcgaggac
     3301 tctaggcgac cttgagcatg gtcgcgaatg gccgtaatcc ttcaacgcct cgtgcgccgg
     3361 gagcggcgat cacgcgctcc acagagcccc cgcgcagtct gtcgtctcga cgtacgagaa
     3421 cagcgagcgg ccgtgagcct gtccggattt ttgtgtgcgg ggctctcaag gcggcgcccg
     3481 ccgtgcttct cggcgaactg atgaacaaga cctgatcaaa ggccgctagg cctgcctctc
     3541 aagaaatcga atgatgcgca agatccactt gtcgcccatc cgcttcttga gttcgcattc
     3601 ccatacgact gccacccccc agccctgccg gcgaagggcg gtgatgttgc gacggtcccg
     3661 tgccacgttt cttcggatct tgtcggccca aaactcgacg ttgctcttcg aggctgcctg
     3721 ccccaggcgg cagtggtggc cgtgccagaa gcagccatgc acgaagacca ccttgttccg
     3781 cgatcggaaa accaagtctg gcgtgccagg cagatccttc tgatggaggc gatatctgaa
     3841 ccccagcgcg tgcagagccg accgaacggt catctccggg cgcgtgtccg aagagcggac
     3901 tctcgacatg agccagctac gtcgttcggg cggcagacgg tcggtcataa gcttcaagct
     3961 gctcaggtaa gcgtgagggt gcgctcccga tcgacaatgc ttgtcttgtg cggtatcgtt
     4021 gggaggtgat gaggatactt agatgaccca gcagaacaaa aaagtaattg accttttcgc
     4081 cggagtgggt ggactgagcc tgggtgctgc gcgcgccgga ttcgatgtcg ccgcggcggt
     4141 tgaattcgac gccaaggccg ccgcccagca cgcggtgaat ttccccagat ccaaccacct
     4201 cgtcgaggac gtcgctgcgc tttctggtga gcgactgctc aatttgtcag gagtagcatc
     4261 gaatgacctg tgtgggctaa tcggcggccc tccgtgtcag ggctttagcg aaatcgggct
     4321 caagggggac ggcgaccctc gcaacgagct tctcgtccat tttttccggc tcgtggccga
     4381 gctcagcccg gcgttcttcc tggcagagaa cgtgccaggc gtgacccacg agcgaaacaa
     4441 gccgatcgtg gagcgagcgc tgtcgctcgt gccgaaaaac tacgtgacgc tgcctccgat
     4501 gacggtcaag gccaacgact acggggcgcc aacaacccga acgcgggtct tcttctgcgg
     4561 ctacgacccg aaccgggtga gtgccctaac cgttgcctcg tttgctccga aggtagggca
     4621 ggacgtgcgt gtgtcacatg cgctgtcagg ccttccgatc atccgctcag attggcagac
     4681 cgaagcacag agctggcgtg ccgtgagaaa gctcgacaag ggcgcttacg aacatcgcat
     4741 ctcgaatcac ataccgttcg gcgtgggcga caaggatgcg attcaggcat acagcaacca
     4801 ttcccgggtt tccggtttcc tcggcaccct tcacacgcct gagacgaaga agcgcttcaa
     4861 ggtgctccaa ccgggcatgg tcgacgccat ctcgcgctcc gttcgtttgc gcatggatgg
     4921 ctattgcccg acgctacggg ctggtaccgg gccggaacgc ggaagctatc aagccattcg
     4981 accgattcat ccgtcctcac ctcgcgtgat cgcgccgcga gaggctgcgc gcctacaagg
     5041 gtttcccgac tggtttcaat tccacccgac aaagtggcac gcattccgcc agatcggaaa
     5101 cagtgttagc ccgatcgtat cggaagcatt gcttacgacg atatacgaag cagtttcata
     5161 agagacatcg tcggtacgga gggtgttatt cgaaggccct gaatcaagac cgtaacacat
     5221 agggagaagg tatggctaca ggagatggcg cgaacgctgc tacggcagcc ccggcgaaga
     5281 agattcacac cgacccgaca aaggatttct tcgtgacgat gatcacgaga gatattcttc
     5341 ttcgggactg catattcgac ttgctggaca actcaatcga cggcgctcga cgcaagccat
     5401 cgaacaatcc ggacaagcct tttgagggac atctaatcga gctgacattc agcgaagaga
     5461 acttcaagat cgccgataac tgcggcggca ttcgcctctc cgacgccatc gactacgcgt
     5521 ttcactttgg aaaaaaggca aatagcccta ccgacgtaag cggcggaata ggactatacg
     5581 gcattggaat gaagcgggcg atcttcaaga ttggccgaat ctgcgtcgtg acgagtcacg
     5641 cggacgatgc gtcgtttaaa gtgtcgatcg acgttgacga atgggagtca cgaactgatt
     5701 gggacttcga ctatgaggac atcgaacgca gcgacgcacg aggcacaacg gttaatatct
     5761 ccaggctgaa cgatggagcc aaggaggcac tgggcgacgc cgtcttcaag aacgacttga
     5821 tgcgcgcaat cgctcgtgac tacgccttct ttattcagca aggattgaag attcaggtag
     5881 gcggcgtaga cgttccgtct tacaagttcc agctccgctc gtccgacagc ttggcgcctt
     5941 ccgtcgagca ttacgtcgac aatggtgtag acgtgcgaat attggccgga cttgtggatg
     6001 agctgccaga cgatattccg gacgaactga agccagacaa agtcgaaagg tatggatggt
     6061 tcgtcatctg caatgaccgc gttgtactgg ctgcgaacaa ggctgatgag acgatatggg
     6121 gagacgacga tttcaacgtc tggcatccgc aatacaacgg attcgctggt tacgtgatct
     6181 ttcaggccgc cgatcaacgg aagcttccgt ggacgactac caagcgcgag ctcgacgcat
     6241 ccagccccct ctatcgccga acaatagcca ggatgaagga agtcactcga ttgttcattc
     6301 aatatacgaa tgctcgaaag gcggatttgg tgactgccaa agccgcggag acccctcggg
     6361 cgcaagtgaa catctccgaa ttgagggagg tacagccttt gaggctaccc gcgatctccg
     6421 ccgccgcgac gcggcccgat atggtcaaca tcacctatcg gcgcgagaag cgagaggtcg
     6481 aagagatcaa gcgccatcta ggttccatcg ccatgtcagc gcgggacgtc gggatcaaga
     6541 cgttcgaata ttaccgtgag gtggagctcg gaaaagaatg agccgcaaca ccccatcatt
     6601 cgaacgaatt gactacctac taagaaccaa caagcacatc gaacgaaagc tcgtctttga
     6661 catcttggat ggagttgccc agcagcttga cttcaagacg cactggtatc ttggctttgg
     6721 gtcgatgtgg ttcggcgact tcaagcttgc gcatcgtcgt ctcggcatag acaagatgat
     6781 ttctatggag cggaaagacc atgcttctcg cgccaagttc aacaggccgt tcgcaggcgt
     6841 caacgtgcag ccgggtgaaa gtagcacgat catcgctggg ctgcctgcag acgcatggga
     6901 caagcccctt gtggcttggc tggactacga tggttacctt aacgaagatg tcgtcaacga
     6961 catcgagctg ctactgagcc gcgcgcggcc caattccgcg atcatcgtga cggtaaatgg
     7021 agcgcgcgga acctatcgag tgaaaaagca aagcggcgta cttacaaggg aagagacttc
     7081 tgtaggggtc gtcgagacgt tcaccggcaa gggcagcgtt tcggagaaat acgaccccaa
     7141 accgaacggc gccgggaact acatcgatgt caaggaggca gaatttcccg agtttttgac
     7201 cgaagcgatt tcagtcttca tgcagcaccg cgtggtctcg ctcgctcgcg aggttgacgg
     7261 tcaacgtctc aagttcgtcc ctttgtatcg gcttcatcac tgcgacggag cggatatggt
     7321 gacagtgggc ggcgctctcg taaccgagga gggggaagcc gcctggcggg actgcgtcgc
     7381 tcgtcatccg ctactgacag atgatgtggg tgaagtcgcg tactgccggc tggatcttgt
     7441 gcctgtgacc gtgaaagaga agatcgcact ggacgaatgc ctaccagatc aaggcaacgg
     7501 ggctcagttc ctggaggctg caaaagccgt gggggtcgcg ctatccgacg aggaaatgac
     7561 taaataccgc aaattctatc ggcatttccc agtgtttgta gaaacgacga tgtaggtttc
     7621 ggggcccgcg ggatggccct acgcccgccg cagaaagcgc ctcgacgagc ccgcagtcgc
     7681 ctacgtacac gagtttccgc cctggtcctc agtgtacgtt ggcggccgcg agcggaatcc
     7741 ggtcatgcgc agggccgcct cggtattgat ccgatcaagc ccctgattca cgatccacac
     7801 agtcgacgtc cgaatacgcg cggccacgct cacgccgccg gcaacgcgag cgcgaacgtc
     7861 gacccggccc ccgccacgct ctgcaccgtg atgcgcccgc ccatcagctg cgcgagcgac
     7921 tgcgacagcg cgaggcccag gcccgcgccg gcgacgccgg agtgctcgcg gccgaggcgg
     7981 ttgaagggct ggaacaggcg tgcgcgctcg gcgggcgtca ggccgtggcc ggtgtcctcg
     8041 acctcgatgc gccactcctc gggctgggcg atcgcacgca gcgtcacgcg gccgccggcg
     8101 cggttgtact tgacggcgtt gctcaggagg ttggtgatga cctggcgcag gcgcgtcgga
     8161 tcggcgcgaa cgcgcgcgac cggcgccagc ggcgacgcgc cggcatcggg atcctcgacg
     8221 cgcagcgcga tgcgcgcggc gtcggcctgc ggccggcacc aggcggcaca cgcctcgaac
     8281 aacggcgcca gcgccacgtt ctgcagctgg atcggcaggt gcgcggcctc ggcgagcgag
     8341 agatcgagca ggtcgccgat catcgcgagc aggtgttcgc cggcgtcgcg gatcatgcgt
     8401 acctgctggc gctgggtggg caacccgtcg agcgcagggt cggtcgacag cagttgcgcg
     8461 aacccgagga ccgcgttcag cggcgtgcgc agctcgtggc tgatgcgcga gaggaacagc
     8521 gtcttcgcgc tgttggcggc ctcggcggcc aggcggtcgc gctgcgcctg ctcgaggtcg
     8581 cgcaggaggg tcgcgtcggt ctgcgtgccg aacatgcggc gcggccggcc gtggcgatcc
     8641 cattcgacga tgcggccgcg ctcctcgatc cagcgccaca gcccgtcggc cgccagcgcg
     8701 cggtagacga cgcggtacat cgccgtctcg ccgcgttcgt gcgcggcgta ggcgcgcgcg
     8761 atcgcgggct ggtcctcggg atggacgatg cggtcccagt cggcctgcgt gtggcccagc
     8821 gtgttcggcg catagcccag catgcgctcg ccctcgtcga tgtcgccgag gacgtcgctc
     8881 gcgatgtccc actcccagac gaaggtgccg gcctcgtgga tggcggtgcg gatcagggcc
     8941 agcgagccgg gcagcgcgtg gccggcgtgc tcgatcaggc cggcgagcag ggccgctgcg
     9001 gtgtccagcg ccggggcgag gcggtcgccc cagtcgtcgc gggccaggcc agcgggcagc
     9061 ggcgtgcgca gctcgccgac gagggtgccc gagtggctgc atggccagcc cg
//
LOCUS       DASVLN010000750         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_92694, whole genome shotgun sequence.
ACCESSION   DASVLN010000750 DASVLN010000000
VERSION     DASVLN010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92694"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1075)
                     /locus_tag="VF453_17815"
     CDS             complement(104..1075)
                     /locus_tag="VF453_17815"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEX7689569.1"
                     /translation="MNRSLKPTVAALATVLSLVVAGAACADEGSSNVEKCSRKLGTIA
                     VAEPSQGWGYLSQYQLGSPAALLRMMVQTSGCFDVVERGAGLHDIQQERALADSGDLR
                     GESNVGKGQMQAADFVMSPNIQIAANNTGGIGGALAGGLLSRVGLGGIAGGLKFKEAQ
                     TSLLVSDVRSSIQVASAEGKASKTDFDIGGWGWAGNTFGAMNGYTSTPEGKVIAASLL
                     DNYNHIVIAIRDNASLIKTSSAAGDVNARGSTHAGVPVKEGEVLMARINGVKVFSGPS
                     KSSKVVGTLSRSDELIANGQEQDGFVQVDSSNFSGWVQRSLVGPGGH"
     gene            1269..>1694
                     /locus_tag="VF453_17820"
     CDS             1269..>1694
                     /locus_tag="VF453_17820"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEX7689570.1"
                     /translation="MRSKPLTKQRLLRWAAALAFALAAAALQWAVRPWFGVRAPFLFF
                     LPAIVAAAAFAGRGGGLLVVAIGLVSAERWLAGDRLWVDTRPDVMSLVVYVGLGLLLT
                     AIGARMRRSSARASAAEQRLLLAGEDTGVGIFDLDLGTRT"
BASE COUNT          200 a          621 c          606 g          267 t
ORIGIN      
        1 aagctgcggc acaccgccgc cgcgtcctcg gccagcgcgt gcgccgacgc catcagcgcc
       61 agcgcggcca gcgccgcgag cgggatcccg cggccgcgcc gcatcagtgg ccgccggggc
      121 cgaccagcga gcgctgcacc cagccgctga agttcgacga gtcgacctgc acgaagccgt
      181 cctgctcctg gccgttggcg atcagctcgt cgctgcgcga cagcgtgccc acgaccttgc
      241 tgctcttcga cggcccggag aacaccttca cgccgttgat gcgcgccatc agcacctcgc
      301 cctccttgac cgggacgccg gcgtgcgtcg agccgcgcgc gttgacgtcg ccggcggcgc
      361 tggaggtctt gatcagcgag gcgttgtcgc ggatcgcgat gacgatgtgg ttgtagttgt
      421 cgagcaggct cgccgcgatg accttgccct cgggcgtgct cgtgtagccg ttcatcgcgc
      481 cgaaggtgtt gccggcccag ccccagccgc cgatgtcgaa gtcggtcttc gaggccttgc
      541 cctcggccga ggcgacctgg atgctcgaac gcacgtccga gacgagcagg ctggtctgcg
      601 cttccttgaa cttcaggccg ccggcgatcc cgcccaggcc gacccggctc agcagcccgc
      661 cggcaagcgc cccgccgatg ccgccggtgt tgttggcggc gatctggatg ttgggcgaca
      721 tcacgaagtc ggccgcctgc atctggccct tgccgacgtt ggactcgccg cgcaggtcgc
      781 cgctgtccgc cagcgcgcgc tcctgctgga tatcgtgcag gccggcgccg cgctcgacga
      841 cgtcgaagca gcccgaggtc tgcaccatca tgcgcagcag cgccgcgggc gagccgagct
      901 ggtactgcga caggtagccc cagccctgcg agggctcggc caccgcgatc gtgccgagct
      961 tgcggctgca cttctcgacg ttggacgagc cctcgtccgc gcacgcggcg ccggcgacga
     1021 cgagggacag gacggtcgcg agcgcggcga cggtcggctt cagggagcgg ttcactgcaa
     1081 tgtcctccag ggtgcgacgc gcggaggtcg gccgccggca tggtgccgtg gaccccgctg
     1141 cgggccgccg aaattgtgcc atcgcactgt cgcgcatccg atccctcgat ggggggatgc
     1201 ccgtgcccgc ggtcgcgcag gcgcgcgctc tgcattaccc ttgcgcctcc ggccggccag
     1261 cgtcctccat gcgatccaag cccctgacca agcaacgact cctgcgctgg gccgcggcgc
     1321 tcgcgttcgc gctcgcggcc gctgcgctgc agtgggcggt gcgtccgtgg ttcggggtgc
     1381 gtgcgccgtt ccttttcttc ctgcccgcga tcgtggcggc ggcagcgttc gcggggcgcg
     1441 gcggcgggct gctcgtcgtc gcgatcgggc tcgtgagcgc cgagcgctgg ctggccggcg
     1501 atcgcctctg ggtcgacacc cggcccgacg tgatgtcgct ggtcgtctac gtcggcctgg
     1561 gcctgctgct gacggcgatc ggggcgcgca tgcggcgctc gagcgcgcgc gcctcggcgg
     1621 ccgagcagcg cctgctgctg gccggcgagg acaccggcgt cggcatcttc gacctcgacc
     1681 tgggtacgcg cacc
//
LOCUS       DASVLN010000751         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_470480, whole genome shotgun sequence.
ACCESSION   DASVLN010000751 DASVLN010000000
VERSION     DASVLN010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470480"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VF453_17825"
     CDS             <1..384
                     /locus_tag="VF453_17825"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7689571.1"
                     /translation="LAARVAGVDAVLDLVGTSTVVDSLHAVRRGGRVCLAGFLGGGAP
                     IERFDPLLHLPSGRHLSFFGSAFVYGTPEYPLADIPFQRLLELAEEGTLDIAPGRVFR
                     FEEIREAHRVLDAQAGGGKMVVLVD"
     gene            470..652
                     /locus_tag="VF453_17830"
     CDS             470..652
                     /locus_tag="VF453_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEX7689572.1"
                     /translation="MTAHYGDKAHDKVHKAMHERKEGTLKSGSGKKVTSKKQAVAIGL
                     SEARKAGGKVPKRSHH"
     gene            709..1137
                     /locus_tag="VF453_17835"
     CDS             709..1137
                     /locus_tag="VF453_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689573.1"
                     /translation="MTAPDDFDQLFDASPAAPAQPLPAPAKPAAGVEARKEVRVRVKW
                     AARVLLAGDRVVPLIVRDVSATGVGLVAESPIASHAVMRIAVAVPHLEVPSRFTTVTG
                     SFRTAHCTVSGPDLLYGGTWVELDPAGRDLIRKWIRRLRP"
     gene            complement(1196..1363)
                     /locus_tag="VF453_17840"
     CDS             complement(1196..1363)
                     /locus_tag="VF453_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX7689574.1"
                     /translation="MLRWALIFAVIAVVAGLLGFTGIEAGAATIARTLFYIFVGLVVV
                     FLVLGFTIFKR"
     gene            1537..>1801
                     /locus_tag="VF453_17845"
     CDS             1537..>1801
                     /locus_tag="VF453_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound PQQ-dependent dehydrogenase,
                     glucose/quinate/shikimate family"
                     /protein_id="HEX7689575.1"
                     /translation="MAANSSAVVRPPFAPLVAGGVSLLFGLALLGLGIALVVAGGSWF
                     PAVSGLGFAIGGGLVAARRAAGLAVVAFVVAAMLAWSLWEAGLA"
BASE COUNT          235 a          659 c          658 g          249 t
ORIGIN      
        1 ctggcggcgc gggtggcggg cgtcgacgcc gtgctcgacc tcgtgggcac gtccaccgtc
       61 gtcgactcgc tgcacgcggt gcggcgcggc ggtcgcgtct gcctcgccgg cttcctcggc
      121 ggcggtgcgc cgatcgagcg cttcgacccg ctcctgcacc tgccgagcgg ccgccacctc
      181 agcttcttcg gcagcgcctt cgtctacggc acgccggagt acccgctagc agacatcccg
      241 ttccagcggc tgctcgagct cgccgaggag ggcaccctgg acatcgcgcc cggccgcgtc
      301 ttccgcttcg aggagatccg cgaggcgcac cgcgtgcttg acgcgcaggc gggcggcggc
      361 aagatggtcg tcctcgtgga ctgaacgcgc ggccgcggac gaccgcgggc gcggccattg
      421 cccgcttcga ggcatcgagg ccccggcctc tcttcatcag gagcacgaca tgacggccca
      481 ctacggcgac aaggcgcacg acaaggtgca caaggcgatg cacgagcgca aggaaggcac
      541 gctcaagagc ggctcgggca agaaggtgac gagcaagaag caggcggtcg ccatcggcct
      601 gagcgaggcc cgcaaggcag gcggcaaggt gcccaagcgc agccatcact gacgaggcgg
      661 cggcgccggc gcggcccgcc ggcgggcacc tgcagcctgg aggcgtgcat gaccgctccc
      721 gacgatttcg accagctctt cgacgcgtcg cctgccgcgc cggcgcagcc gttgccggcc
      781 ccggccaagc cggcggccgg cgtggaggcg cgcaaggaag tccgcgtgcg ggtcaagtgg
      841 gcggcgcgcg tgctgctcgc cggcgaccgc gtcgtgccgc tgatcgtgcg tgacgtgagc
      901 gcgaccggcg tcggcctggt cgccgaatcg ccgatcgcct cgcacgcggt gatgcgcatc
      961 gccgtggccg tcccgcacct ggaggtgccc agccgcttca cgacggtcac gggctcgttc
     1021 cgtaccgcgc actgcacggt gagcgggccc gacctgctgt acggcggcac ctgggtcgag
     1081 ctcgatccgg cgggtcgcga cctgatccgc aagtggatcc gccggctgcg gccatgagcc
     1141 ctcgtccggc cggacggagg ccgcggcgcg agtgccggcg gcgcgcgacg ccgcgctaac
     1201 gcttgaagat cgtgaatccc agcaccagga acacgaccac caggccgacg aagatgtaga
     1261 acagcgtgcg cgcgatcgtc gcggcgccgg cctcgatgcc cgtgaatccg agcaggccgg
     1321 cgaccaccgc gatgacggcg aagatgagcg cccatctgag catggcatga ctcccgtttg
     1381 cgcgccggac gtcggcgctt cgggcccctt cggcaacccg catgccgacc gcgacccgac
     1441 cccgtcgtcc aggcgcgcgg tgcgaggcac ggcgattgcc aacggcgcac cgcgtcgtcc
     1501 ggccccgtcc ggctggagac ggaaccgatt ccacccatgg ccgccaactc ctccgccgtc
     1561 gttcgccctc ccttcgcccc gctcgtcgcc ggcggcgtgt cgctgctgtt cggcctcgcg
     1621 ctgctcgggc tcgggatcgc gctggtcgtc gccggcggct cgtggtttcc ggcagtctcg
     1681 ggcctgggct tcgcgatcgg cggcgggctg gtcgcggcgc ggcgggcggc agggttggcc
     1741 gtcgtcgcct tcgtcgtcgc ggcgatgctg gcgtggagcc tgtgggaagc gggtctcgcc
     1801 t
//
LOCUS       DASVLN010000752         3000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_414484, whole genome shotgun sequence.
ACCESSION   DASVLN010000752 DASVLN010000000
VERSION     DASVLN010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414484"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VF453_17850"
     CDS             complement(<1..1177)
                     /locus_tag="VF453_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689576.1"
                     /translation="MNPAPAAPDTLAAFEPLNDCEREMCRVAPLGGSVRPPGRPAGPA
                     DVTVRAALVRLVAIERDPALRLHESGLRLVGMIVDGDLDLGGCEVRRGLSFERCGLGY
                     VGLFDARLAKLAFENCRIGRLAGDRGGLGALFLRDCIVEAGVHLMGVRIDGDLDCSRS
                     RITAQDPADAQDFGRRQALALDNTKVGGAFFLRETRIDGSVTADSMAVAQLMSASRCR
                     VERGRWAMHSATIGGDLELDGAAFSTQGPGQALLRPASLLLAGARIGGSLLMTAGFEA
                     DGPVDLSDARVAGHLGVRGACFRGVHAFAIVAQRLAVDGVFDLDPTTRFLRPESAPGA
                     PPALDFTAATLGGISCEGGAWPRGNRVHGLRYGGIVGATPTRARWWIDWLQRQVPEDL
                     "
     gene            complement(1336..1593)
                     /locus_tag="VF453_17855"
     CDS             complement(1336..1593)
                     /locus_tag="VF453_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HEX7689577.1"
                     /translation="MTDSAAPAARPDLPDHLSTDPRSPHHVAAVFEHEIGIRFNGKER
                     FDVEEYCLSEGWIKVPAGKTRDRRGQPLMIKLKGQVEAFYR"
     gene            1784..>3000
                     /locus_tag="VF453_17860"
     CDS             1784..>3000
                     /locus_tag="VF453_17860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7689578.1"
                     /translation="MSPTRRHRFLAEGRAQLRLAGPIILSQVAQVLAGLVDTVMSGRA
                     GAREQAVVGIGVALWVPVFLFLMNVVQSLSPIVAHHFGAGDHRAVVRDTQDGVWLAAW
                     SGALPLALVPLAPAALEFAGIDPGLAQRTGVFLWGIGLGLPAAMVFRALSFYSASLNH
                     TRPMMMLALLGLGVNAALNDVLIHGRLGLPAMGGAGCGWATGIGMWVGLAAFAAWTAR
                     SRAYAAWYLWRDWRRPRWEAQKRLLHLGLPMGAAGLAEAGAFSSVAVLVGRFGAVQIA
                     AHQVAINFAAMVFMLPMGLSAAMAVRIGHALGAGDAREARHVAWTGMALAVLIALAAM
                     GPIFGLRHLVAAVYSNDAEVRALAAQLLLFASAWILFDATQVCAMGALRGYKVTFAPM
                     LMMLLAFWGVGVPV"
BASE COUNT          356 a         1093 c         1138 g          413 t
ORIGIN      
        1 cgaggtcttc cggcacctgg cgctgcagcc agtcgatcca ccagcgagcg cgggtcggcg
       61 tcgcgccgac gatcccgccg tagcgcaggc catggacgcg attgccgcgc ggccaggcgc
      121 cgccctcgca ggagatgccg cccagcgtcg cggccgtgaa gtcgagggcc ggcggcgcgc
      181 ccggcgccga ctcggggcgg aggaaacggg tggtgggatc gaggtcgaag acgccgtcga
      241 ccgccagccg ctgcgccacg atggcgaacg cgtgcacgcc gcggaagcac gcaccgcgca
      301 cgcccaggtg cccggcgacg cgcgcgtcgc tcaggtcgac cgggccgtcc gcctcgaagc
      361 cggccgtcat cagcagcgag ccgccgatgc gcgcccccgc cagcagcagc gaggccggcc
      421 gcaggagcgc ctgtcccggg ccctgggtcg agaaggcggc gccatcgagc tcgaggtcgc
      481 cgccgatcgt ggccgagtgc atcgcccatc gtccgcgttc cacccggcag cgcgaggcgg
      541 acatcagctg cgccaccgcc atcgagtcgg cggtgacgct gccgtcgatc cgcgtttcgc
      601 gcaggaagaa cgcaccgccg accttggtgt tgtccagggc gagcgcctgg cggcggccga
      661 agtcctgggc atcggcggga tcctgcgccg tgatccggga ccgggagcag tcgaggtcgc
      721 cgtcgatgcg cacgcccatc aggtgcacgc cggcctccac gatgcaatcg cgcaggaaca
      781 gcgcgcccag gccgccacgg tcgcccgcca gccggccgat gcggcagttc tcgaacgcga
      841 gcttggcgag ccgggcgtcg aacagcccga cgtagcccag gccgcagcgc tcgaacgaca
      901 ggccgcgccg cacctcgcag ccgccgaggt cgaggtcgcc gtcgacgatc atgccgacga
      961 gccgcagccc cgactcatgc aggcgcagcg ccgggtcgcg ctcaatggcg acgagccgca
     1021 ccagcgccgc gcgcacggtg acgtcggcgg gtcccgccgg ccggcccggc ggtcgcacgc
     1081 tgccccccag cggcgcgacg cggcacatct cgcgctcgca gtcgttgagc ggctcgaaag
     1141 cggccagggt atcgggcgcg gccggcgcgg ggttcatcgg gcgacctccg tccgcgcgtg
     1201 gagttcggcg cggtggccga tgctaagcag ctcgcccgcg gcggcgcatc gcggaaaacg
     1261 cccgaagcgg gcccgtcgcg ggccccgaac gtgggatggc gagacggcgc gaccgcgcgc
     1321 agccgtcgcc gcgactcagc ggtagaacgc ctccacctgc cccttgagct tgatcatcag
     1381 cggctggccg cggcggtcgc gggtcttgcc ggccggcacc ttgatccagc cctcgctgag
     1441 gcagtactcc tcgacgtcga agcgctcctt gccattgaag cggatgccga tctcgtgctc
     1501 gaagacggcg gccacgtggt gcgggctgcg cgggtcggtc gagaggtggt cgggcaggtc
     1561 gggacgggcg gcaggcgcgg cggagtcggt catgggataa gtccggtgga ggctcggcga
     1621 tgcgagcgaa acacggcatt ttcgcggatt cggcgcgggc attccggcgg gcgccctgcg
     1681 cgacggcgcg gcggtgatac attgccgccc ctcgccggcc ggctcgcccc atgagcccgg
     1741 cgcgcgcgat cctcgccctc gcccacccgt cgccgccctg cccgtgtccc cgacccgccg
     1801 ccaccgcttc ctcgccgagg gccgcgccca gctccgcctg gccggcccca tcatcctgtc
     1861 gcaggtcgcg caggtgctgg ccgggctcgt cgacacggtg atgtcgggcc gggccggtgc
     1921 gcgcgagcag gcggtcgtgg gcatcggcgt cgccctgtgg gtgccggtgt tcctgttcct
     1981 gatgaacgtc gtgcagtcgt tgtcgccgat cgtcgcccac cacttcggcg ccggcgacca
     2041 ccgcgccgtc gtgcgcgaca cgcaggacgg cgtctggctg gcggcgtggt cgggcgcgct
     2101 gccgctggcg ctggtgccgc tcgcgcccgc ggcgctcgag ttcgccggca tcgatccggg
     2161 gctcgcgcag cgcaccggcg tgttcctttg gggcatcggg ctcgggctgc cggcggcgat
     2221 ggtgttccgc gcgctgtcgt tctattccgc gagcctgaac cacacgcggc cgatgatgat
     2281 gctggccttg ctcgggctcg gcgtcaacgc ggcgctcaac gacgtcctga tccacggccg
     2341 cctcgggctg ccggcgatgg gcggcgccgg ctgcggctgg gcgacgggga tcggcatgtg
     2401 ggtcggcctg gcagcgttcg cggcgtggac ggcgcgctcg cgcgcctacg ccgcctggta
     2461 cctgtggcgc gactggcgcc gtccgcgctg ggaggcccag aagcgcctgc tgcacctggg
     2521 gctgccgatg ggcgccgcag ggcttgccga ggccggcgca ttctccagcg tcgcggtcct
     2581 ggtgggccgg ttcggcgcgg tgcagatcgc ggcgcaccag gtcgccatca acttcgccgc
     2641 gatggtcttc atgctgccga tggggctgtc cgccgcgatg gcggtgcgca tcggccacgc
     2701 gctgggcgcc ggcgacgcgc gcgaggcgcg ccacgtcgcc tggacgggca tggccctggc
     2761 ggtgctcatc gcgctggcgg cgatggggcc gatcttcggc ctgcgccacc tggtggccgc
     2821 cgtctacagc aacgacgccg aggtgcgcgc gctcgcggcg cagctgctgc tgttcgcgtc
     2881 ggcgtggatc ctgttcgacg cgacacaggt ctgcgcgatg ggcgcgctgc gcggctacaa
     2941 ggtcaccttc gcgccgatgc tgatgatgct gctcgcgttc tggggcgtgg gcgtgccggt
//
LOCUS       DASVLN010000753         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_149204, whole genome shotgun sequence.
ACCESSION   DASVLN010000753 DASVLN010000000
VERSION     DASVLN010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149204"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..1127)
                     /locus_tag="VF453_17865"
     CDS             complement(303..1127)
                     /locus_tag="VF453_17865"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEX7689579.1"
                     /translation="MDYLLGDLQGCCDPLDELLAAIDFSPSRDRLHVLGDLVNRGPTS
                     LRVLERLSGLGDAAQCLLGNHDLHLLAVAHGVRKASKGDTLDAILADPRRDAWMDWLR
                     TRRLAGEACGWLLVHAGVLPSWSRQDTLERAAEVEALLRSDDLRGFLAVMYGNEPRRW
                     DPALAGPDRWRVIVNTLTRMRLCSADDVLDLESKESPGENAPPGMRPWFEHANRRTAG
                     VPIAFGHWSTLGLVDTPTLLATDTGCVWGGALTAVRIDGGRREVFQVKCPQARKPG"
     gene            1306..2607
                     /locus_tag="VF453_17870"
     CDS             1306..2607
                     /locus_tag="VF453_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7689580.1"
                     /translation="MDVALLIFLIVLNAGFAMTEMALTTSRKARLQVLAESGARGVEA
                     AIDLHEHPTKFLSTVQVGITSISVLNGIVGDAAFSEPLGIWLHASFGMAERAAHVTAT
                     ALVVLIITVLTIIFGELVPKRIGQLFPETVAALFAPTMQWLSTATRPLVMLLSATTDG
                     VLRILGIRGNAKRGVTEEEIAASLEEGLDAGVIEAQEHQMVRNVFRLDDRQIGSMMIP
                     RAEIRWLDATAPIADVLKAVVEEAHARYPVCRGGLDDVIGVVTAQGLLKPLAEGRVPV
                     LTEELQPPVFVPETLSGMELLEHFRTNAAQLVFVVDEYGAVQGVITLRDVLEAITGEF
                     SPGTGDDAWAVQREDGSWLMDGLIPVPELKDRLAVKDLPEEDRGRYNTLAGMIMLLLG
                     RLPRTADTVDWGDWRFEVVDLDGKRVDKVLVSRIPSPGEQP"
     gene            2604..3347
                     /locus_tag="VF453_17875"
     CDS             2604..3347
                     /locus_tag="VF453_17875"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEX7689581.1"
                     /translation="MILTTLHKEPTALDTVLHRDLRLDTQVNAAPLMAPYTSFMITVG
                     EFAEAARDFPILFVKAAPDEEGKDTIAPVIVFGMRPGENVFLKDGQWQAGYLPALLRA
                     YPFTMARIENSDRWAMVFDNSWKGFSRTEGTPLFDEKGEASEMLNNVHKFVQELEADV
                     ERTRQACVYLQQKNLLKPMRFDATLANGETLSVDGFMTLDEEAFAKLGDKDIAELFRN
                     GLMFILHMHRASLSNMRRLLERRMAQQAN"
BASE COUNT          520 a         1257 c         1248 g          486 t
ORIGIN      
        1 cgagcgtgcg cgcggcgccg gcgcgcgcgt cgcgcagcgc gaccaggctc agcgcggtga
       61 tcgcgaccac cagccccagc accaggcaga agccgagcgc caggcggcgc gagagccgga
      121 tcgagtcgag gcgaaagggc atgaggacct cctcgtcgtc gtgtgcgtgc gcctgcgcgc
      181 ggcgcagggc ggggcgcgct cgtgcggccc gcgaccgcga gcttgaacgt ccgccggcgc
      241 ggcgtcaatc gggcgcggcc gcaggtcgtg ctggtacggg gtgaggtatc gaggagtcct
      301 gctcagcccg gcttgcgagc ctgcgggcac ttgacctgga acacctcgcg ccggccgccg
      361 tcgatgcgca ccgccgtcag cgcgccgccc cagacgcagc cggtgtcggt cgccagcagc
      421 gtcggcgtgt cgaccaggcc cagcgtcgac cagtggccga acgcgatcgg cacgccggcc
      481 gtgcggcggt tcgcgtgctc gaaccacggg cgcatgccgg gcggggcgtt ctcgccgggg
      541 ctttccttgg actcgagatc cagcacgtcg tcggccgagc acagccgcat gcgcgtgagc
      601 gtgttgacga tgacgcgcca gcggtcgggc ccggcgagcg ccggatccca gcgccgcggc
      661 tcgttgccgt acatcaccgc caggaagccg cgcaggtcgt ccgagcgcag cagcgcttcc
      721 acttcggcgg cgcgctccag cgtgtcctgc cggctccacg acggcagcac cccggcatgc
      781 acgagcagcc agccgcacgc ctcgcccgcc aggcggcggg tgcgcagcca gtccatccag
      841 gcgtcgcggc gcgggtcggc gaggatcgcg tcgagcgtgt cgcccttcga cgccttgcgc
      901 acgccgtggg cgacggccag caggtgcagg tcatggttgc cgagcaggca ctgcgcggcg
      961 tcgcccaggc ccgacaggcg ctcgaggact cgcagcgagg tcggcccgcg gttgacgagg
     1021 tcgcccagca cgtgcaggcg gtcgcgcgac ggcgagaagt cgatggcggc gagcagctcg
     1081 tcgagcgggt cgcagcagcc ctggaggtcg cctagcagat aatccatgcc tgcgattctg
     1141 ctacgctcgc gggctttggc ccggggtgtg cgcctcgccg cggatccccg atcgattttc
     1201 cccgctgcct tccctgctgc ccaagggttt tcctcgaccc ggcagccgcg gcgccgcgcc
     1261 ccggcgcgcc gcccgcgaag aagcccgcag cgccccgtag ttcgcatgga cgtcgccctc
     1321 ctcatcttcc tgatcgtgct gaacgccggt ttcgcgatga ccgaaatggc gttgacgacg
     1381 agccgcaagg cgcgcctgca ggtgctggcg gagtcgggcg cgcgcggcgt cgaggcggcg
     1441 atcgacctcc acgagcatcc gaccaagttc ctctccacgg tgcaggtggg catcacctcg
     1501 atcagcgtgc tcaacggcat cgtcggcgac gccgccttct cggagccgct gggcatctgg
     1561 ctgcacgcga gcttcgggat ggccgagcgc gccgcgcacg tgacggccac cgcgctcgtg
     1621 gtcctgatca tcaccgtgct gaccatcatc ttcggcgagc tcgtgcccaa gcgcatcggc
     1681 cagttgttcc ccgagaccgt ggccgcgctg ttcgcgccga cgatgcagtg gctgtcgacc
     1741 gccacgcgcc cgctcgtgat gctgctgtcg gcgacgacgg acggggtgct gcgcatcctc
     1801 ggcatccgcg gcaatgccaa gcgcggggtg accgaggagg agatcgccgc cagcctcgag
     1861 gaaggcctgg acgccggcgt gatcgaggcg caggagcacc agatggtgcg caacgtcttc
     1921 cgcctcgacg accgccagat cggctcgatg atgatcccgc gcgccgagat ccgctggctc
     1981 gacgccacgg cgccgatcgc cgacgtgctc aaggccgtcg tcgaggaggc gcacgcgcgc
     2041 taccccgtgt gccgcggcgg gctggacgac gtgatcggcg tcgtgaccgc gcagggcctg
     2101 ctcaagccgc tcgccgaagg ccgggtgccg gtgctgaccg aggagttgca gccgccggtc
     2161 ttcgtgccgg agacgctgtc ggggatggaa ctgctcgagc acttccgcac gaacgccgcg
     2221 cagctcgtgt tcgtggtcga cgagtacggc gcggtgcagg gcgtgatcac gctgcgcgac
     2281 gtgctggagg cgatcaccgg cgaattcagc cccggcaccg gcgacgacgc ctgggccgtg
     2341 cagcgcgagg acggcagctg gctcatggac gggctcatcc cggtgcccga gctgaaggac
     2401 cgcctcgccg tgaaggacct gcccgaggag gaccgcgggc gctacaacac cctggccggc
     2461 atgatcatgc tgctgctggg ccgcctgccc cggacggcgg acaccgtcga ctggggcgac
     2521 tggcgcttcg aggtcgtcga tctcgacggc aagcgagtgg acaaggtgct cgtgagccgt
     2581 atcccttccc ctggagaaca accatgatcc tcacgaccct gcacaaggaa ccgaccgccc
     2641 tcgacaccgt cctgcaccgc gacctgcgcc tggacacgca ggtcaacgcc gcgccgctga
     2701 tggcgccgta cacctcgttc atgatcaccg tgggcgagtt cgccgaggcc gcgcgcgact
     2761 tcccgatcct gttcgtgaag gccgcgcccg acgaggaggg caaggacacg atcgcccccg
     2821 tcatcgtctt cggcatgcgc ccgggcgaga acgtcttcct gaaggacggc cagtggcagg
     2881 cgggctacct gccggcgctg ctgcgcgcct accccttcac gatggcgcgc atcgagaact
     2941 ccgaccgctg ggcgatggtc ttcgacaact cgtggaaggg cttctcgcgc accgagggca
     3001 cgccgctgtt cgacgagaag ggcgaggcga gcgagatgct caacaacgtg cacaagttcg
     3061 tgcaggagct cgaggccgac gtcgagcgca cgcgccaggc ctgcgtctac ctgcagcaga
     3121 agaacctgct caagccgatg cgcttcgacg ccacgctggc caacggcgag acgctgtccg
     3181 tcgacggctt catgacgctc gacgaggagg ccttcgccaa gctcggcgac aaggacatcg
     3241 ccgagctgtt ccgcaacggg ctcatgttca tcctgcacat gcaccgcgcc tcgctgtcca
     3301 acatgcgccg cctgctcgag cgccggatgg cgcagcaggc gaactgaagg cggcgcgtat
     3361 cagtcgccgg ccccgggctg ccagcccagc cggcgcgaca gctcctgcgc cgcgggccgc
     3421 agcacgcggg cgacggcgcc gtcggggcgg gcgtcgaagg tggcgctcgg cccgatcgcc
     3481 gtcaggccca gcacgagccg cccgcgcgcg t
//
LOCUS       DASVLN010000754        13559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_200199, whole genome shotgun sequence.
ACCESSION   DASVLN010000754 DASVLN010000000
VERSION     DASVLN010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200199"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VF453_17880"
     CDS             <1..406
                     /locus_tag="VF453_17880"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX7689582.1"
                     /translation="RDAALDPADLLVTAVGVNDVVGQTRAAVFLRALDELHAVAVARA
                     GITHAWHCGVPRMAEFPLLPAPLRWILGRDALALDHALARHLAADPSRLHVPLPATPE
                     GGAPPGWMAEDGFHPGPAGYRHWGRSIVEAVG"
     gene            complement(423..986)
                     /locus_tag="VF453_17885"
     CDS             complement(423..986)
                     /locus_tag="VF453_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689583.1"
                     /translation="MEHAAPPPSWVERAVVEIRASSTYRSKVADEFYETLPVPAWSAQ
                     HNWLVVGFGAIHRRAPPPGPFETRMPHMACTVSYPDGLQHWSLDEAVAPLWPVRPGLA
                     DAAIPPPVIDGLQERKRQYYSALSQALAVDAFTGHPPADPVAACAAARQVRALFLPAA
                     MFPNLGKVYAKPVADMDAWLATHCAKP"
     gene            complement(1008..1577)
                     /locus_tag="VF453_17890"
     CDS             complement(1008..1577)
                     /locus_tag="VF453_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689584.1"
                     /translation="MDFAATPQPSWVEHAVVEMRRSPEYQARIVDEWYETVPVPAWND
                     TRNWLVVGFGKTKRRGPGPQSPVEVRAPHIACVVSYPDGQRQWAVDNTALRVWPTHAG
                     DPEPVFPPLAVGGSQQRREHYYALLSSALGHGAFAAHTPPDPVAACTAARAAREAFLP
                     AATYASLAPIYATPLHDMDGWLSAHCPKP"
     gene            complement(1591..2280)
                     /locus_tag="VF453_17895"
     CDS             complement(1591..2280)
                     /locus_tag="VF453_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689585.1"
                     /translation="MDDASEQSLGSALGSAWNSVKNFFAEIPSHLPCIPCLFKRAGIV
                     GPNWTKAANDVFPGQQSYQNCGVQSSRQIVEQAKGKLSKTELQFLDDAIASCGAEKDA
                     TNHPADSGGTGAPQRQCIMKQYGVDSSIENATVSNVDDALRNGKGVIMSSDADTLWGT
                     QGIAASGAGRHAVLLTNGLYDSNGKLTGVGVNDTGIGRQYTLTVSQLDACLKSGSGVI
                     NVTNARIWPSN"
     gene            complement(2287..2634)
                     /locus_tag="VF453_17900"
     CDS             complement(2287..2634)
                     /locus_tag="VF453_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689586.1"
                     /translation="MTRIDRQQFAALGAAQHERFRQRLVSFIDGQFGPPPPGAQYRSS
                     AEVADSALEFAQTVGAMTEAQVARIAILLVGVNRRQSPPDEVARVRAALLHPDKTPDE
                     RIEAAAAVLGITG"
     gene            complement(2754..3158)
                     /locus_tag="VF453_17905"
     CDS             complement(2754..3158)
                     /locus_tag="VF453_17905"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7689587.1"
                     /translation="MTDAATPPTALPSDKELVLRVIPMPSDANANGDIFGGWIMAQVD
                     LAGSVLPSRIAKGRVTTVAVNQFVFKQPVSMGDLLSFYATVERIGNTSITVRVEVYAE
                     RNPADLQVVKVTEANLTYVAIDREGKPRAIPR"
     gene            3450..5321
                     /locus_tag="VF453_17910"
     CDS             3450..5321
                     /locus_tag="VF453_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX7689588.1"
                     /translation="MHPSPPSSGPPTGRAADSRGELATIRRLLPYFWEYRLRMAIALA
                     CLVAAKVASVAVPLLLKRLVDALGVSVSDPRALLVLPVGLLLGYAGLRLATSIFTELR
                     EIVFARATFGAAKKVSLATFEHLNALSLRFHLERQTGGLTRDIERGTRALQTLVSYSL
                     YTIIPTLVELAFVLVLLGTRFDVGYVWITLGALVLYGTYTITITEWRTKYRKAMNEAD
                     SRANTKAIDALINFETVKYFNNERFESERYERGLESYRAAQMKSQATLSMLNTGQQLI
                     IAASLVALLWRATGGVVAHRMTLGDFVMVNALLIQLYVPLNFLGVLYREVKQGLTDLE
                     RMFALLEKEREIADAPGATELRVSGGAVVFDRVSFAYDPARPILHDVSFEIPAGATVA
                     VVGPSGAGKSTLARLLYRFYDVTGGAIRIDGQDLRAVTQNSLRKAIGIVPQDTVLFND
                     TVGYNIAYGRPGATQGEVEAAARAARIHEFIRATPQGYETTVGERGLKLSGGEKQRIA
                     IARTLLKNPPLMIFDEATSALDSANERAIQAELAQAAQGRTTLVIAHRLSTVVNAAQI
                     LVMADGRIVERGTHAELLALDGRYAHMWRLQQEHGEAVADDPARLGDEAFEAAAAAK"
     gene            5383..6312
                     /locus_tag="VF453_17915"
     CDS             5383..6312
                     /locus_tag="VF453_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7689589.1"
                     /translation="METKWLEDFVSLAETRSFSRSAQLRHVTQPAFSRRIQALEAWAG
                     LDLVDRSSYPTRLTPAGETFHAQALEVLGSLQATRNMMRGLQAGGADMIEFAAPHTLT
                     FTFFPAWLMDLRRRFGEIKSRLIALNVHDAVMRMTEGSCDLLVAYHHASQPLQLSPDR
                     YEMLSLGQETLAPYAKAGADGAPLFRLSATGGSGRIPFLGYASGAYMARMVDQILKSS
                     PTLASLDTVYETDMAEGLKAMAIEGHGVAFLPSSSVRKELRTRRLVSAAPLNDDSGHA
                     YELTMEVRMYRERPELARHPKPGALALWEFLKH"
     gene            6482..7486
                     /locus_tag="VF453_17920"
     CDS             6482..7486
                     /locus_tag="VF453_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7689590.1"
                     /translation="MHISLHADDIRTVERAAPRARPGATASLLLAAMVAAALPGTAGA
                     KGAASAADASTELPPGPHPVLDRIKASGRIVLAHREGSVPFSYYDADKRPIGYAIDLC
                     KDLAETVRKRLGMKSLNIAYMPVNTSTRIPAITDNKADLECGTTTNNAERRQKVAFTV
                     PHYITGSRYLVRADSPIAELSQFEGHVLVATAGSTQFKQIDQANRDRLLNIKVLEVAD
                     NAKAMQMVTSGQADGFALDDVQLYGLIAESPNPAKFKVVGKFLTIEPLAIMMSKDDPA
                     FKKIVDEEMKRMIRAGEAEALYDKWFMKPIPPKNVAMNLPMNYLLRDSWKYPSDIVGN
                     "
     gene            7666..8571
                     /locus_tag="VF453_17925"
     CDS             7666..8571
                     /locus_tag="VF453_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7689591.1"
                     /translation="MMKTLTVAATLLAAAVSAHAQTTDTLKKIKDTGTIVMGVRGSSG
                     ALSYTLGNGQYVGFHVELCQRAIDDIGKQLGKKIEVKYQEVTSANRIPLVQNGTVDIE
                     CGSTTNNEAREKDVAFAHTAYVEEVRILTKTNSGVKDIKDLNGKTVATTTGTTSVQLL
                     RKNERATGIDFKEVFGKDHADSFQLLESGRADAFVMDGQIVMGLASKSKNPADFHTVG
                     PALSVEPIAIMVRKDDPAFLAAVNKTLDGLMKSGEMAKIYDKWFMQPIPPTNTKVGLP
                     ASEYTKAAWANPNSNPTEWYVAHAK"
     gene            8787..9503
                     /locus_tag="VF453_17930"
     CDS             8787..9503
                     /locus_tag="VF453_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7689592.1"
                     /translation="MCKSTVDETMYSSCFAGKGDLTYLQWLLQAWGWTLTVALVALVI
                     AMVLGLMIGVIRTLPSKGLVLFGEAWTEFFRNIPILIQLLVWYHIIPEIFPTLKSVSP
                     VVLACIGLGLFTSARIGQQVKAGIATIPKGQRYAGLAMGLTLAQTYRYVLLPVALRIV
                     LPTITSEAMNIIKNSSVAYAIGVVEMISFATQAGEETSMHGTMYFGATVLYFVSAFAI
                     NRAAALVEKRTRLAGALGGK"
     gene            9505..10200
                     /locus_tag="VF453_17935"
     CDS             9505..10200
                     /locus_tag="VF453_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7689593.1"
                     /translation="MALNLDFSFLTADVIKANIVPGFYYSVQLTFVTMVGGVILGTLV
                     ALMRLSSKPWLQRIAAVYVDTLRSIPLLMVILWFYLLIPSGFYALIGGSFGENHRPEF
                     AAVITFTFFEAAYYSEIMRAGIQSVPRGQVYAGYAVGMTYAQCMRLVVLPQAFRNMLP
                     VLLTQTIVLFQDTSLVYAIGAYDFLKGFLTAGQVYNRPDEVLLPAAACYFVISFGLSM
                     LVRRLQAKIQIIR"
     gene            10210..10944
                     /locus_tag="VF453_17940"
     CDS             10210..10944
                     /locus_tag="VF453_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7689594.1"
                     /translation="MIDIKNISKWYGSFQVLTDCTTTINKGDVVVVCGPSGSGKSTLI
                     KTVNALEPIQKGDIVVDGTSITDPKTNLPKLRSRVGMVFQHFELFPHLSITDNLTLAQ
                     TKVLGRNKDDAVKRGLKMLDRVGLMAHKDKFPGQLSGGQQQRVAIARALSMDPIVMLF
                     DEPTSALDPEMVGEVLDVMVQLAQEGMTMMCVTHEMGFARRVSNKVIFMDAGRIVEDC
                     TKDDFFGNPEARSPRAKEFLSKILQH"
     gene            11023..12057
                     /gene="pyrC"
                     /locus_tag="VF453_17945"
     CDS             11023..12057
                     /gene="pyrC"
                     /locus_tag="VF453_17945"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7689595.1"
                     /translation="MTTELTITRPDDWHLHVRDGAAMAAVLPHTARQFGRAIIMPNLR
                     PPITTTAQAVAYRERLLAALPAGSDFQPLMTLYLTDNLPPEEIRRAKEAGIVAVKLYP
                     AGATTNSDAGVTDIRKTYATLEALQREGLKLLVHGEVTSADIDLFDREAVFIEQVFQP
                     LRRDFPELKIVFEHITTREAAQYVAQSDACTAATITAHHLLYNRNAIFQGGLRPHYYC
                     LPVLKREIHRQALVAAAASGSAKFFLGTDSAPHASVMKESSVCGAGCFTAMSALELYA
                     EAFEAAGALDKLEGFASFHGPDFYGLPRNTGTVTLRKEPLQLPESIPFGDAAQLKPLR
                     AGETLAWRQV"
     gene            12054..12500
                     /locus_tag="VF453_17950"
     CDS             12054..12500
                     /locus_tag="VF453_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7689596.1"
                     /translation="MSASALAFEDVVAADADALVAIRIAAMRESLERLGRFDPRRARD
                     RFLAGFDPDCTQHLVVACERVGFIVVRERGGELLLDHLYVLPAHQGRGLGSAALASVF
                     VRADAARLPVRVGALRGSESNRFYARHGFVLVEEAEFDNYYVRPSR"
     gene            complement(12562..12747)
                     /locus_tag="VF453_17955"
     CDS             complement(12562..12747)
                     /locus_tag="VF453_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689597.1"
                     /translation="MTQKKNEQRPLGRTNWERVLKQTDEDIERQAASDPDAPLVDVNA
                     HFTKPGKKPANRQSKPD"
     gene            complement(12728..12919)
                     /locus_tag="VF453_17960"
     CDS             complement(12728..12919)
                     /locus_tag="VF453_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689598.1"
                     /translation="MPEKSYTLEELRKLKSLTDQERLDRMTEEEIDRNAREDPDSVVL
                     DDEQLERLRKVEKHDAEKK"
     gene            complement(12931..13218)
                     /locus_tag="VF453_17965"
                     /pseudo
     CDS             complement(12931..13218)
                     /locus_tag="VF453_17965"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
BASE COUNT         2144 a         4750 c         4496 g         2169 t
ORIGIN      
        1 gcgcgacgcc gcgctcgatc cggccgacct gctggtcacc gccgtcggcg tcaacgacgt
       61 cgtcggccag acccgggccg cggtcttcct gcgcgcgctc gacgagttgc acgcggtcgc
      121 cgtcgcgcgc gcgggcatca cgcacgcctg gcactgcggc gtgccgcgca tggccgagtt
      181 cccgctgctg ccggcgcccc tgcgctggat cctcggccgc gacgcgcttg ccctcgacca
      241 tgcactcgcc cgccacctgg ccgccgaccc gtcgcgcctg cacgtccccc tgccggccac
      301 gcccgagggc ggcgccccgc ccggctggat ggccgaggat ggcttccatc cggggccggc
      361 cggctaccgg cactgggggc gcagcatcgt agaggcggtg ggctgacctc gcgcggcgtc
      421 gctcagggct tcgcgcagtg ggtggcgagc caggcgtcca tgtctgcgac cggcttggcg
      481 tagaccttgc cgaggttcgg aaacatggcg gccggcagga agagggcccg cacctggcga
      541 gcggccgcgc aggcggcgac tggatctgcc ggtggatgtc ccgtgaacgc gtcgaccgcg
      601 agcgcctgcg acagcgccga gtagtactgt cgcttgcgtt cctgcaaccc gtcgatcacc
      661 ggcggcggga tggccgcgtc ggcgagtcca gggcgtaccg gccagagcgg tgcgaccgct
      721 tcgtccagcg accaatgctg caggccgtcg ggatagctca cggtgcaggc catgtgcggc
      781 atccgcgttt cgaagggccc tggcggaggc gcacgtcggt ggatcgcgcc gaagccgacc
      841 acgagccagt tgtgctgtgc gctccatgcg ggaacgggca gcgtctcgta gaactcgtcg
      901 gcgaccttgc tgcggtacgt cgacgatgcc cggatttcga cgacggcccg ctcgacccag
      961 gaaggcggcg gcgcggcgtg ctccatgcga ctgctccttc ggaagcacta aggcttcggg
     1021 caatgggcag atagccagcc gtccatgtcg tgcagcggcg tcgcgtaaat gggagcgagc
     1081 gaggcgtaag tggcggccgg caggaatgcc tcgcgcgcgg cacgagccgc cgtgcaggct
     1141 gccaccgggt cgggcggcgt gtgggccgcg aaggccccgt gcccgagggc actcgagagc
     1201 agcgcgtaat agtgctcgcg ccgctgctgc gagccgccga ctgccagcgg cgggaacacg
     1261 ggctccggat cgcctgcgtg cgtcggccag acgcgtagcg ccgtgttgtc gacggcccac
     1321 tgtcgctgcc cgtcgggata gctcaccacg caagcgatgt gcggtgcacg cacctcaacg
     1381 ggcgactgcg ggccgggccc cctgcgcttg gtcttcccga agcccacgac cagccagttg
     1441 cgcgtgtcgt tccaggcagg cacgggcacc gtctcatacc actcgtcgac aatgcgcgcc
     1501 tgatattcgg gcgatcgccg catctccacg acggcgtgct cgacccagct gggctgggga
     1561 gtggctgcga agtccattcg attcctttga tcagttgctt ggccagatgc gggcgttggt
     1621 gacattgatg acgcccgagc cgctcttgag gcaagcgtcc aattgactga ccgtaagagt
     1681 gtattggcgg ccgataccgg tgtcgttcac accgacgcct gtcagctttc cgttgctgtc
     1741 gtaaagaccg ttggtgagca acacggcgtg tctgcccgct cccgaggcgg cgatgccttg
     1801 cgtgccccaa agggtgtccg cgtcggaaga catgatgacg cccttgccgt tgcggagggc
     1861 gtcgtcaacg ttcgacacag tagcgttctc gatgctcgag tccacgccgt actgcttcat
     1921 gatgcactgc cgctgcgggg caccagtacc gccggagtca gcagggtgat tcgtggcatc
     1981 cttttcagcg ccgcaactcg cgatcgcgtc gtctaggaac tgcaactccg tcttgctgag
     2041 ctttcccttg gcctgctcga cgatctggcg cgaagactgg actccgcaat tctgatacga
     2101 ctgctgcccc gggaagacgt cattcgcggc cttcgtccaa ttcggcccaa cgatgcccgc
     2161 tctcttgaac aggcacggaa tgcacggcag atggctcgga atctcggcga agaagttctt
     2221 caccgagttc cacgcggagc ccagggcgct gcccagcgat tgctcggatg cgtcgtccat
     2281 ggcgcgtcag ccggtgatgc cgagcacggc ggcggcggct tcgatccgct cgtcgggcgt
     2341 cttgtcggga tgcagcagtg ccgcgcgcac gcgggcgacc tcgtcgggcg gcgactggcg
     2401 gcggttgact cccaccagca ggatcgcgat gcgcgcgacc tgggcctcgg tcatcgcccc
     2461 gacggtctgt gcgaactcca gggccgagtc ggcgacctcc gcggagctgc ggtactgggc
     2521 gccggggggc ggcggcccga actggccgtc gatgaaggag acgaggcgtt ggcggaaacg
     2581 ctcatgttgc gcggcgccca gcgccgcgaa ctgctggcgg tcgatgcgcg tcatgccgca
     2641 cgctcgccgc ggtcgatccg tctgttcaag cgatgctcct ccgggagccg gcatttgaac
     2701 cgacgcggcc gtttcggcga acccccccga tgggtgatcg cggacgcggc ccgtcagcgg
     2761 gggatcgccc tcggcttgcc ctcgcgatcg atcgcgacgt aggtcaggtt cgcctcggtc
     2821 accttcacga cctgcaggtc cgccgggttg cgctcggcat agacctcgac gcgcaccgtg
     2881 atcgacgtgt tgccgatgcg ttcgaccgtg gcgtagaagc tcagcaggtc gcccatggac
     2941 accggctgct tgaagacgaa ctggttgacg gccaccgtcg tcacgcggcc cttggcgatg
     3001 cggctgggca gcaccgagcc ggcgaggtcg acctgggcca tgatccagcc gccgaagatg
     3061 tcgccgttgg cgttggcgtc cgagggcatc gggatgacgc gcagcacgag ttccttgtcg
     3121 gagggcaggg cggtcggggg cgtggcggcg tcggtcatcg ggtgggcgct ctctggtgga
     3181 tgcgcgattg tccatcaaag ggttttccct gtgcccgcag ccgcgccgcg ctccgacggg
     3241 gtttcgtgac ttccggccgc gtacactgcg gcgtcccgct cgcgcgggca cgacaacaaa
     3301 cgccgtccgc ccgcgcggac acggcgaggg tgggcagggc ggccgccgcg cggcggtcgc
     3361 ctcgtctctt cgcggtcggc ccgcacgccc gtccggcgcc gacccgcgat ccttgctatc
     3421 gtggccgccg tccccacctg cgccgtccga tgcatccctc cccgccgtcc tccggtcccc
     3481 cgaccggccg cgccgccgat tcgcgcggcg agctcgccac catccgccgg ctgctgccct
     3541 acttctggga gtaccggctg cgcatggcga tcgcgctcgc ctgcctggtc gcggccaagg
     3601 tcgcgagcgt ggccgtgccc ctgttgctca agcgcctggt cgacgcgctc ggcgtgagcg
     3661 tgagcgaccc gcgcgcgttg ctggtgctgc cggtcggcct gctgctgggc tatgcggggc
     3721 tgcggctggc gacgtcgatc ttcaccgagc tgcgcgagat cgtcttcgcg cgcgcgacct
     3781 tcggcgccgc caagaaggtc tcgctcgcga ccttcgagca cctcaacgcg ctgtcgctgc
     3841 ggttccatct cgagcgccag accggcggcc tcacgcgcga catcgagcgc ggcacgcggg
     3901 cgctgcagac gctcgtgtcg tactcgctct acacgatcat cccgacgctc gtcgagctcg
     3961 cgttcgtgct ggtgctgctg ggcacgcgct tcgacgtcgg atacgtctgg atcacgctcg
     4021 gcgcgctggt gctgtacggc acctacacga tcacgatcac cgagtggcgc accaagtacc
     4081 gcaaggcgat gaacgaggcg gactcccgtg ccaacacgaa ggcgatcgac gcgctgatca
     4141 acttcgagac ggtcaagtac ttcaacaacg agcgcttcga gtccgagcgc tacgagcgcg
     4201 gcctggagtc gtaccgcgcg gcgcagatga agtcgcaggc gacgctgtcg atgctcaaca
     4261 cggggcagca gctgatcatc gccgcctcgc tcgtcgcgct gctgtggcgg gcgaccggcg
     4321 gcgtcgtcgc gcaccgcatg acgctgggcg acttcgtgat ggtcaacgcg ctgctgatcc
     4381 agctgtacgt gccgctgaat ttcctcggcg tgctgtaccg cgaggtcaag cagggcctga
     4441 ccgacctgga gcgcatgttc gcgctgctgg aaaaggagcg cgagatcgcc gatgcgcccg
     4501 gcgcgaccga gctgcgcgtg agcgggggcg ccgtcgtctt cgaccgcgtg agcttcgcct
     4561 acgaccccgc gcgtcccatc ctgcacgacg tgagcttcga gatcccggcc ggcgcgacgg
     4621 tggccgtcgt cggcccgagc ggcgccggca agagcacgct cgcccggctg ctgtaccggt
     4681 tctacgacgt gaccggcggc gcgatccgga tcgacggcca ggacctgcgt gccgtcaccc
     4741 agaacagcct gcgcaaggcg atcggcatcg tgccgcagga caccgtgctg ttcaacgaca
     4801 cggtcggcta caacatcgcc tacggccgcc cgggcgcgac gcagggcgag gtcgaggccg
     4861 cggcccgcgc cgcgcgcatc cacgagttca tccgcgcgac gccccagggc tacgagacca
     4921 ccgtgggcga gcgcggcctc aagctgtcgg gcggcgagaa gcagcgcatc gcgatcgcgc
     4981 gcacgctgct gaagaacccg ccgctcatga tcttcgacga ggcgacctcg gcgctcgact
     5041 cggccaacga gcgcgccatc caggccgagc tcgcccaggc cgcgcagggg cgcacgacgc
     5101 tcgtgatcgc acaccgcctg tcgaccgtcg tcaacgcggc ccagatcctc gtgatggccg
     5161 acggccgcat cgtggagcgc ggcacccacg ccgagctgct ggccctggac ggccgctacg
     5221 cccacatgtg gcgcctgcag caggagcacg gcgaggccgt cgccgacgac cccgcgaggc
     5281 tgggcgacga ggccttcgaa gccgccgcgg ctgccaagta agtctcgctt tcgcctcggg
     5341 agaaccctga ccggcgcgcg gcgcgaaggc taaactgccg cggtggaaac caagtggctc
     5401 gaagacttcg tctccctcgc ggagacgcgc agcttctcgc gctcggcgca gctgcggcac
     5461 gtgacccagc cggcgttctc gcgccgcatc caggccctgg aggcgtgggc ggggctcgac
     5521 ctcgtcgacc gctcgtccta tccgacgcgc ctgacgcccg ccggcgagac cttccacgcc
     5581 caggcgctcg aggtgctagg cagcctgcag gcgacgcgca acatgatgcg cgggctgcag
     5641 gccggcggcg ccgacatgat cgagttcgcc gccccgcaca cgctcacctt cacgttcttc
     5701 ccggcatggc tgatggacct gcgccgacgc ttcggcgaga tcaagagccg cctgatcgcc
     5761 ctcaacgtgc atgacgccgt gatgcgcatg accgagggca gctgcgacct gctcgtggcc
     5821 taccaccacg cgtcgcagcc cctgcagctg agccccgacc gctacgagat gctgagcctc
     5881 ggccaggaga cgctcgcgcc ctacgccaag gccggggcgg acggcgcgcc gctgttccgg
     5941 ctgtcggcga ccggcggctc cggccgcatc cccttcctcg gctacgcctc gggcgcctac
     6001 atggcgcgca tggtcgacca gatcctgaag tcctcgccga cgcttgcgag cctggacacc
     6061 gtctacgaga ccgacatggc cgagggcctg aaggcgatgg caatcgaggg ccacggcgtc
     6121 gcgttcctgc cctcgagctc cgtcaggaag gaactgcgca cgcgccggct ggtctcggcc
     6181 gcgccgctga acgacgacag cggccatgcc tacgagctga cgatggaggt ccgcatgtac
     6241 cgcgagcgcc ccgaactcgc gcggcatccg aagccgggcg cgctcgcgct ctgggagttc
     6301 ctgaaacatt gacggcggac actgcccggc catcgtccgt ctccgggccg gtacgggcgg
     6361 ggcggattcg acccctccga acgattaggt gataaccctt ggctggcatg caaatcttgc
     6421 atggccgacc cttcaattgg cattggcgcc gctcgacggc gccgcattag agttcgcgac
     6481 catgcacatt tcgttgcacg ccgatgacat ccgcaccgtc gaacgcgccg ccccgcgcgc
     6541 ccggcccgga gcgacggctt ccctcctcct cgcggcgatg gtcgccgcgg cgctgcccgg
     6601 cacggccggc gcgaagggag cggcttccgc ggcggacgcg tcgaccgagc tgccgcccgg
     6661 cccgcatccg gtgctcgacc gcatcaaggc gtcgggccgc atcgtgctgg cgcatcgcga
     6721 gggctcggtg cccttctcgt actacgacgc cgacaagcgc ccgatcggct acgccatcga
     6781 cctgtgcaag gacctcgccg agacggtgcg caagcgcctg ggcatgaagt cgctcaacat
     6841 cgcctacatg ccggtgaaca cgtccacgcg catcccggcg atcaccgaca acaaggccga
     6901 cctcgagtgc ggcacgacga ccaacaacgc cgaacgccgc cagaaggtcg ccttcacggt
     6961 gccgcactac atcaccggct cgcgctacct cgtgcgcgcg gacagcccca tcgcggagct
     7021 ctcgcagttc gagggccacg tgctggtggc gaccgccggc tcgacgcagt tcaagcagat
     7081 cgaccaggcc aaccgcgacc ggctcctgaa catcaaggtg ctggaggtgg cggacaacgc
     7141 caaggcgatg cagatggtca cgagcggcca ggccgacggc ttcgcgctcg acgacgtgca
     7201 gctctacggg ctcatcgctg aatcgccgaa tccggccaag ttcaaggtgg tcggcaagtt
     7261 cctgaccatc gagccgctcg ccatcatgat gagcaaggac gaccccgcgt tcaagaagat
     7321 cgtcgacgag gagatgaagc gcatgatccg cgcgggcgag gccgaggcgc tctacgacaa
     7381 gtggttcatg aagccgatcc cgccgaagaa cgtcgccatg aacctgccca tgaactacct
     7441 gctgagggac tcgtggaaat acccgtcgga catcgtcggc aattgagcct cgggcgcccg
     7501 cgcacggtac gtgctttcac gaacgcgcgg ccgccggcat aagtggctag actccggcag
     7561 tccgcgcgcg cccaccccgc gcggccccct gccgcaaccc atttctccga tcggcgccaa
     7621 cgccgacttt tcaaccgtcc ccccgaacat ccaggagcag acacaatgat gaagaccctc
     7681 acggttgccg ccacgctgct ggccgcggcc gtctcggccc acgcccagac caccgacacg
     7741 ctcaagaaga tcaaggacac cggcaccatc gtgatgggcg tgcgcggctc gtccggcgcg
     7801 ctgtcgtaca ccctgggcaa cggccagtac gtcggcttcc acgtcgagct gtgccagcgc
     7861 gcgatcgacg acatcggcaa gcagctgggc aagaagatcg aggtcaagta ccaggaagtg
     7921 acgtcggcca accgcatccc gctggtgcag aacggcaccg tcgacatcga gtgcggctcg
     7981 acgaccaaca acgaggcgcg cgagaaggat gtcgccttcg cccataccgc ctacgtcgag
     8041 gaggtgcgca tcctcacgaa gacgaactcg ggcgtcaagg acatcaagga cctgaacggc
     8101 aagaccgtcg cgacgacgac cggcacgacc tcggtgcagc tgctgcgcaa gaacgagcgc
     8161 gccaccggca tcgacttcaa ggaagtgttc ggcaaggacc acgccgacag cttccagctg
     8221 ctcgagagcg gccgcgccga cgcgttcgtg atggacggcc agatcgtgat gggcctggcc
     8281 tcgaagtcga agaacccggc cgacttccac accgtcggcc ccgcgctgtc ggtcgagccg
     8341 atcgcgatca tggtgcgcaa ggacgacccg gccttcctgg cggccgtcaa caagacgctg
     8401 gacggcctga tgaagtcggg cgagatggcc aagatctacg acaagtggtt catgcagccg
     8461 atcccgccga ccaacaccaa ggtcggcctg ccggcgtccg agtacacgaa ggcggcctgg
     8521 gccaatccga acagcaaccc gaccgagtgg tacgtggcgc acgccaagtg agccagacgg
     8581 ccgcggcgcg ccgatgaggc gcccgccgcg gccggttcga tgagacaaac ctgcgtgcgg
     8641 acccggcgac gggcccgcac gctgcacaat gggcggcgcc tgccatcgca tccggcgcgg
     8701 cgcagcgagg ccggcggacg ctgccggcgg acaacgagga gcagagggtg acctgggcgc
     8761 agcagataca cgatagcgcg gcggtgatgt gcaagagcac ggtcgacgag accatgtact
     8821 cgagctgctt cgccggcaag ggggacctga cctacctgca gtggctgctg caggcctggg
     8881 gctggacgct gaccgtcgcg ctcgtggcgc tggtgatcgc gatggtgctg ggcctgatga
     8941 tcggcgtcat ccgcacgctg cccagcaagg gactggtgct gttcggcgag gcctggaccg
     9001 agttcttccg caacatcccg atcctgatcc agctgctggt ctggtaccac atcatcccgg
     9061 agatcttccc gacgctgaag tcggtctcgc ccgtcgtgct cgcgtgcatc gggctgggct
     9121 tgttcacgtc cgcgcggatc ggccagcagg tcaaggccgg catcgcgacc atccccaaag
     9181 gccagcgcta cgcgggcctg gcgatgggcc tcacgctcgc ccagacctac cgctacgtgc
     9241 tgctgcccgt ggcgctgcgc atcgtgctgc cgacgatcac ctcggaggcg atgaacatca
     9301 tcaagaactc gtcggtcgcc tacgcgatcg gcgtggtcga gatgatctcc ttcgccaccc
     9361 aggcgggcga ggagacctcg atgcacggca cgatgtactt cggcgcgacc gtgctgtact
     9421 tcgtctcggc cttcgcgatc aaccgcgcgg ccgccctcgt ggagaagcgc acccgcctcg
     9481 ccggcgcgct ggggggcaag tgagatggcg ctgaacctcg acttctcctt cctgacggcc
     9541 gacgtcatca aggccaacat cgtgcccggg ttctactact cggtgcaact gacgttcgtc
     9601 accatggtcg gcggcgtcat cctgggcacg ctggtcgcgc tgatgcggct gtcgtccaag
     9661 ccctggctgc agcgcatcgc ggcggtctac gtggacacct tgcggtccat accgctgctg
     9721 atggtgatcc tgtggttcta cctgctgatc ccgagcggct tctacgcgct gatcggcggc
     9781 agcttcggcg agaaccaccg gcccgagttc gccgccgtca tcaccttcac gttcttcgag
     9841 gcggcgtact actcggagat catgcgcgcg ggcatccagt cggtgccgcg cggccaggtc
     9901 tacgccggct acgccgtggg catgacctac gcccagtgca tgcgcctcgt ggtgctgccg
     9961 caggccttcc gcaacatgct gccggtgctg ctgacgcaga ccatcgtgct gttccaggat
    10021 acctccctgg tgtatgcgat cggcgcctac gacttcctca agggcttcct gaccgcgggc
    10081 caggtctaca accgcccgga cgaggtcctg ctgccggccg ccgcctgcta tttcgtcatc
    10141 tcgttcggcc tgtcgatgct ggtgcgtcgc ctgcaggcca agatccagat catccgctga
    10201 gagtccccca tgatcgacat caagaacatc tccaagtggt acggcagctt ccaggtgctg
    10261 accgattgca ccaccacgat caacaagggg gacgtggtgg tcgtctgcgg gccgtcgggc
    10321 tcgggcaagt ccacgctgat caagacggtc aacgcgctgg agccgatcca gaagggcgac
    10381 atcgtcgtcg acggcacctc catcaccgac cccaagacca acctgcccaa gctgcgctcg
    10441 cgcgtgggca tggtgttcca gcatttcgag ctgttcccgc acctgtccat caccgacaac
    10501 ctgacgctcg cgcagaccaa ggtgctgggc cgcaacaagg acgacgccgt caagcgcggg
    10561 ctcaagatgc tcgaccgcgt cgggctgatg gcgcacaagg acaagttccc gggccagctg
    10621 tccggcggcc agcagcagcg cgtggcgatc gcgcgcgcgc tgtcgatgga tccgatcgtg
    10681 atgctgttcg acgagccgac ctcggcgctc gaccccgaga tggtcggcga ggtgctggac
    10741 gtgatggtgc agctcgcgca ggaaggcatg accatgatgt gcgtgacgca cgagatgggc
    10801 ttcgccaggc gcgtgagcaa caaggtcatc ttcatggacg cgggcaggat cgtcgaggac
    10861 tgcaccaagg acgacttctt cggcaatccc gaagcgcgct cgccgcgcgc caaggagttc
    10921 ctctcgaaga tcctccaaca ctaggccctg cggtcgccag cgctcgacag cgcccgctgc
    10981 gaccatgccg cgggccggct ggcgtggtga gaaaatcgcg ggatgaccac cgaactcacg
    11041 atcacccggc cggacgactg gcacctgcac gtgcgcgacg gcgccgcgat ggcggccgtg
    11101 ctgccgcaca ccgcgcgcca gttcggccgc gcgatcatca tgcccaacct gcggccgccg
    11161 atcacgacga cggcgcaggc cgtggcctat cgcgagcgcc tcctggccgc gctgcccgcc
    11221 ggcagcgact tccagccgct gatgacgctg tacctgaccg acaacctgcc gcccgaggag
    11281 atccgtcgcg cgaaggaggc gggcatcgtc gcggtcaagc tgtacccggc cggcgcgacg
    11341 accaacagcg acgccggcgt caccgacatc cgcaagacct acgcgacgct cgaggcgctg
    11401 cagcgcgaag ggctgaagct gctcgtgcac ggcgaggtga ccagcgccga catcgacctg
    11461 ttcgaccgcg aagcggtgtt catcgagcag gtgttccagc cgctgcgtcg cgacttcccc
    11521 gagctgaaga tcgtcttcga gcacatcacg acgcgcgagg ccgcccagta cgtcgcccag
    11581 tcggacgcgt gcacggcggc gacgatcacc gcgcaccacc tgctctacaa ccgcaacgcg
    11641 atcttccagg gcggcctgcg cccgcactac tactgcctgc cggtgctcaa gcgggagatc
    11701 caccgccagg cgctcgtcgc ggcggcggcc tcgggcagcg cgaagttctt cctgggcacc
    11761 gacagcgcgc cgcacgcctc ggtgatgaag gaaagctcgg tgtgcggcgc cggctgcttc
    11821 acggcgatgt cggcgctgga gctctacgcc gaggccttcg aggccgcggg cgcgctcgac
    11881 aagctggaag gcttcgcgag cttccacggc cccgacttct acggcctgcc gcgcaacacg
    11941 ggcaccgtga cgctgcgcaa ggagccgctg cagctgcccg agagcatccc cttcggcgac
    12001 gccgcgcagc tgaagccgct gcgcgccggc gagacgctcg cctggcgcca ggtgtgagcg
    12061 cgtcggcgct cgcgttcgag gatgtcgtcg ccgcggacgc cgacgcgctc gtcgcgatcc
    12121 gcatcgccgc gatgcgagag agcctcgagc gcctcggccg cttcgacccg cggcgcgccc
    12181 gcgatcgttt cctcgccggt ttcgatcccg actgcacgca gcacctcgtc gtcgcgtgcg
    12241 agcgcgtggg cttcatcgtc gtgcgcgagc gcggcggcga gctgctgctc gaccacctct
    12301 acgtgctgcc cgcgcaccag ggccgagggc ttggctcggc ggcgctggcg tcggtcttcg
    12361 tgcgcgccga cgccgcgcgg ctgccggtgc gcgtgggcgc actgcggggc agcgagtcga
    12421 accgcttcta cgcgcggcac ggtttcgtgc tcgtcgagga ggcggagttc gacaactact
    12481 acgtcaggcc gagtcgatag gccggtttgc tcgcgatgtc gctgacttcg cggaacgtgt
    12541 tgcccgaggg cgtcgacccc ctcagtcggg cttcgattgg cgattcgcgg gcttcttgcc
    12601 cggcttggtg aaatgcgcgt tcacgtccac gagcggcgcg tcggggtcgg atgccgcctg
    12661 ccgttcgatg tcctcgtcgg tctgcttgag gacgcgctcc cagttcgtgc gtccgagcgg
    12721 gcgctgttca ttttttttct gcgtcatgct tttctacctt ccgaaggcgt tccaactgct
    12781 cgtcatccaa aacgaccgag tcgggatctt cgcgagcgtt acgatcgatt tcctcttcgg
    12841 tcattcgatc caagcgctcc tgatcggtca ggcttttcag cttccgaagc tcttccaatg
    12901 tatagctttt ttcgggcata actttctcca ctatgcgccg gacttgcgcg acagatacaa
    12961 ctgccgctcc tttcgattgg ctctacgagc agatatgagc cgtcgctcct ttccctgctc
    13021 tcgcaaagtg aacacaacca ccagcatgtt cggcgccacc tcgccgatcg caaaaagacg
    13081 ctgttcgcct tagtcgtgac ggtcgtccac gccttcgatc cgatattcgt cttcgaagat
    13141 gaatgctgca tcgtagaagt cgatgccgtg cttctcgatg ttcgaagcgc gcttggcctc
    13201 gtcccactga tacttcatgc tgagccgcaa tctgtccgaa agcgagcacg ctagcaaaag
    13261 cccgttgggc gatcgatccc cattttaggg tggagcctcg caggtgcgcc gagtgcgcca
    13321 gtgttgcaaa ttgcacgcga ggacgcatcc cgcggggctg ccgcgctgaa ctggcgcccg
    13381 gatcggccga taaacaaaga ccagccccac ggccgagcag gacgatccgc ccgggcgtcc
    13441 accggcgttc gcgccgccct ttgcagccgc tgccctgccc gacgtgtcct cgcccgactc
    13501 cgacttcgcc gagctcgccg ccatcgaggc cgcgcgcctc gcggcgctgc gcgcgctcg
//
LOCUS       DASVLN010000755         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_308647, whole genome shotgun sequence.
ACCESSION   DASVLN010000755 DASVLN010000000
VERSION     DASVLN010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308647"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF453_17970"
     CDS             complement(<1..327)
                     /locus_tag="VF453_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689599.1"
                     /translation="MPLPLTPRAHAALAACALSLACPRLPAADPPPTWTLPVRVVASG
                     LPGAHGVREVGRFHAGGPFATDPAFLLATQPGQVLDPTRVLVAVDGNGGMVLSIDSRA
                     RGAVVPP"
     gene            complement(414..968)
                     /locus_tag="VF453_17975"
     CDS             complement(414..968)
                     /locus_tag="VF453_17975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX7689600.1"
                     /translation="MSEVTQLIARARAGDRASLDRLFELLYPELRRIAHRRLAHDGRN
                     GPLQTTALVNECYLKFAQREGLSPSDRAHFLAYAATVMRSVIVDAARAARADHRGGGV
                     RHVTLDTSIGDSVGAAADEVLDVHAALEELQGIDARLVRVVEMRYFVGMTDLEIADAL
                     GVTDRTVRRDWEKARLLLAHAMRP"
     gene            1416..2561
                     /locus_tag="VF453_17980"
     CDS             1416..2561
                     /locus_tag="VF453_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7689601.1"
                     /translation="MAGGVAWRRGAADAAAYVTVPASRGSIVQTVNSSGAVNPVTTVQ
                     VGSYVSGVIQTISCDFNTRVTAGQLCARIDPRPYQTVVDQEVASVATARAQLAKDRAN
                     LSFTGILARRDAGLLERGVVSQETADTSASNYAQARAQLALDEASVAQHEAQLKAARI
                     NLGYADIVSPVSGTVVSRNVTQGQTVAASFQTPTLFLIATDLTRMQVDTNVSEADIGR
                     VAVGDAARFTVTAYPDREFAGVVQQVRQAPQSVQNVVTYDVVVGVRNDDLALMPGMTA
                     SMRIVSRHVDDVLRVPEQALRLRPRAAKASEDDASAGGSGARRAVWVLRDGQPTRVAV
                     TVGLDDERFAEITGGDLHAGDRVIVAERAGDRVDVPSLSPGLAAGRR"
     gene            2566..>3322
                     /locus_tag="VF453_17985"
     CDS             2566..>3322
                     /locus_tag="VF453_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689602.1"
                     /translation="MGAACIEARDLRRTYRIGDVQVDALRGVSLVVERGEFVAIMGSS
                     GSGKSTLMAILGCLDSPTGGSYRFEGTDVATLGEPALARLRSERLGFVFQSFNLLPRT
                     SALDNVALPLIYARDAVPVERRMRRARDALGLLGLSERAGNTPAQLSGGQQQRVAIAR
                     ALVNSPAVLLADEPTGNLDTRTSHEIMETLVRLNREQGVTIVVVTHERDIADYAGRVV
                     TMRDGQIVADERKPAPAAGAAGTPPAAPSGAARE"
BASE COUNT          447 a         1205 c         1258 g          412 t
ORIGIN      
        1 cggcggcacg acggcgccgc gggcccgcga gtcgatggac agcaccatgc cgccgttgcc
       61 gtcgaccgcc accagcacgc gcgtcgggtc gagcacctgc cccggctgcg tggccagcag
      121 gaaggcggga tcggtggcga acggccctcc cgcgtggaac cggccgacct cgcgcacgcc
      181 gtgcgcgccc ggaaggccgg aggcgacgac gcgcacggga agcgtccagg tgggcggagg
      241 atccgcggca ggcaagcgcg gacacgcgag cgacagggcg caggcggcga gcgccgcgtg
      301 ggcgcgcggc gtgaggggca ggggcatggc ggactttcga cggacgggcg gggtccgcgg
      361 ccggaccccc gacccgatgt acgtcgcggc cgtcgctatc cggacattcg atgtcagggc
      421 cgcatcgcgt gcgcgagcag cagccgcgcc ttctcccagt cgcggcgcac cgtgcggtcg
      481 gtcacgccca gcgcgtcggc gatctcgagg tcggtcatgc ccacgaagta gcgcatctcg
      541 acgacgcgga cgagacgcgc atcgatgccc tgcagctcct cgagcgcggc atggacgtcc
      601 agcacttcgt cggccgcggc gccgaccgag tcgccgatcg aggtgtccag cgtcacgtgg
      661 cggacgccgc ctccgcgatg gtcggcgcgc gccgcgcgcg cggcgtcgac gatgaccgag
      721 cgcatgacgg tggcagcgta ggcgaggaaa tgcgcacggt cggaggggct cagtccctcg
      781 cgttgcgcga acttcaggta gcactcgttc accagcgccg tcgtctgcag ggggccgttg
      841 cgtccgtcgt gcgcgagccg ccggtgcgcg atgcggcgca gctccggata gagcaactcg
      901 aagaggcggt cgagcgaggc ccggtcgccg gcccgcgccc gtgcgatgag ttgcgtcacc
      961 tcgctcatgc gtccgctccg tcgctgccgt ggatcgaggg ccatgatagc ggcggcgcgg
     1021 acgcgcggcc gggccgtcac cgaccctcga acgcgcctgt cccgcggagg tccggcggtc
     1081 gcgcagggcc ggatcgcttg cgccaacgca accccgtcca cgggcgcggg tcgcaggatg
     1141 gggacctcct cgtccaccca cgcgcgcccg tccgcgcccc ggaaccatga tgccctctcg
     1201 acacgccgtc gtcctcgtcg accgggcgca aggcgcctga ccgggcatcg cagcgatctc
     1261 gcggcgaccg cggacgggac tcgactgcaa ggcggcccat gtcggtaccg atggcccttc
     1321 cggatgcccg cccgtcgacg tcgcccggcg cggggccgtc ccgtgcgcgc aggtgggcgt
     1381 gggcggctct cgcgttcctc gcgctcgccg cggcggtggc cggcggggtc gcatggcgac
     1441 gcggcgccgc cgacgcggcg gcctacgtga cggtgccggc ttcccgcgga agcatcgtgc
     1501 agacggtcaa ctccagcggg gcggtcaatc cggtcaccac ggtgcaggtg ggcagctacg
     1561 tgtcgggcgt gatccagacg atcagctgcg acttcaacac ccgcgtcacg gccgggcagc
     1621 tgtgcgcccg gatcgacccg cggccttacc agaccgtcgt cgaccaggag gtcgcctcgg
     1681 tggccaccgc gcgcgcccag ctcgccaagg atcgcgccaa cctgagcttc acgggcatcc
     1741 tcgcgcggcg cgacgcgggg ctgctcgagc ggggcgtggt ctcgcaggag acggccgaca
     1801 cgtccgccag caactacgcc caggcgcgcg cgcagctcgc gctcgacgag gcgagcgtcg
     1861 cgcagcacga ggcgcagctg aaggcggccc gcatcaacct cggctacgcc gacatcgtct
     1921 cgccggtgag cggcacggtg gtctcgcgca acgtgacgca ggggcagacc gtcgcggcga
     1981 gcttccagac gcccacgctg ttcctcatcg cgacggacct cacgcgcatg caggtcgaca
     2041 ccaacgtcag cgaggccgac atcggccggg tcgccgtggg cgatgccgcc cggttcacgg
     2101 tcaccgccta tcccgaccgc gagttcgccg gcgtggtgca gcaggtgcgg caggcgccgc
     2161 agtccgtgca gaacgtcgtg acctacgacg tcgtggtcgg cgtgcgcaac gacgacctgg
     2221 cgctcatgcc gggcatgacg gcgtcgatgc gcatcgtcag ccggcacgtc gacgacgtgc
     2281 tgcgcgtgcc ggagcaggcg ctgcgcctgc gtccgagggc cgcgaaggcg agcgaggacg
     2341 acgcgtcggc cggcggcagc ggcgcgcggc gcgccgtgtg ggtgctgcgc gacggccagc
     2401 cgacacgcgt ggccgtgacg gtcggcctgg acgacgagcg cttcgccgag atcacgggcg
     2461 gcgacctgca cgccggcgac cgcgtgatcg tggcggaacg cgcgggcgac cgcgtggacg
     2521 tgccgtcgct ttccccgggc ctggcggccg ggcggcggta gggccatggg cgcggcctgc
     2581 atcgaggcgc gcgacctgcg gcgcacctac cgcatcggcg acgtgcaggt ggacgccctg
     2641 cgcggcgtga gcctggtcgt cgagcgcggc gagttcgtcg ccatcatggg gtcgtcgggg
     2701 tcggggaagt ccacgctgat ggccatcctc ggctgcctcg acagcccgac cggcggctcc
     2761 taccggttcg aaggcaccga cgtcgcgacg ctcggcgagc cggccctggc gcgcctgcgc
     2821 agcgagcgcc tgggcttcgt gttccagagc ttcaacctgc tgccgcgcac cagcgccctg
     2881 gacaacgtcg cgctgccgtt gatctatgcg cgcgacgccg ttccggtcga gcgccgcatg
     2941 cgtcgcgcgc gcgatgcgct gggcttgctc gggctgtcgg agcgggcggg caacacgccg
     3001 gcgcagctgt cgggcggcca gcagcagcgc gtggccatcg cgcgggcgct cgtcaactcg
     3061 cccgccgtcc tgctcgccga cgagccgacc ggcaacctcg acacgcgcac gtcgcacgag
     3121 atcatggaga cgctcgtgcg gctgaaccgc gagcagggcg tgacgatcgt cgtcgtgacg
     3181 cacgagcgcg acatcgcgga ctacgccggc cgcgtggtca cgatgcgcga cgggcagatc
     3241 gtcgccgacg agcgcaagcc ggctcctgca gcgggcgccg caggcacgcc gccggcggcg
     3301 ccttcgggtg cggcgcggga ac
//
LOCUS       DASVLN010000756         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_38240, whole genome shotgun sequence.
ACCESSION   DASVLN010000756 DASVLN010000000
VERSION     DASVLN010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38240"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VF453_17990"
     CDS             <1..399
                     /locus_tag="VF453_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7689603.1"
                     /translation="PAGLCEWVRARVAAGEGGEVPAQWTSVAGTRRLRCSLHPASAED
                     EWLVVLTESDHGAAVEALALRFRLTAREAEVLYWVAQGKTNRDIGEILGTSPKTVTKH
                     LEHVFRKLGVETRTAAASRVIGTLPELLRR"
     gene            525..1790
                     /gene="urtA"
                     /locus_tag="VF453_17995"
     CDS             525..1790
                     /gene="urtA"
                     /locus_tag="VF453_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEX7689604.1"
                     /translation="MTLSRRLFNLSLVATAAAVASTGAQAADTIKVGILHSLSGTMAI
                     SETVLKDTALMTIAEINAKGGVLGKQLEPVVVDPASNWPLFAEKARQLIAQDKVAVVF
                     GCWTSVSRKSVLPVFEELNSLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEYLMSK
                     DGGSAKRFVLLGTDYVYPRTTNKILRAFLHSKGVADADIMEEYTPFGHSDYQSIIARI
                     KKFSGEGKKTAVVSTINGDSNVPFYKELGNQGLKATEVPVVAFSVGEEELRGVDTKPL
                     VGNLAAWNYFMSIKNPTNAAFIKQWSDYAKKNNLPADKDKPLTNDPMEATYVGIHMWA
                     QAVEKAKSTDTDKVIAAMAGQTFKAPSGFVLEMDPKNHHLHKPVFIGEVRADGQFNVV
                     WKTPAPIKAQPWSPYIPEDKGKKDEPEKK"
     gene            1914..>2418
                     /locus_tag="VF453_18000"
     CDS             1914..>2418
                     /locus_tag="VF453_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEX7689605.1"
                     /translation="MPRFTIDALLRGVAAIALGIALSPAWALDASQARAIAVGDGDAR
                     IAALDAAVERADPGLAGFAQALLDDAVKTTATGVYVVRDDKAVDAVTGAPATLPDDAQ
                     DVTSNNRMRGALQAALSGLKLLSKDAGQRHAAIDELSSADPDPALLPVLDRAWAAETD
                     GHARRQLD"
BASE COUNT          427 a          833 c          835 g          323 t
ORIGIN      
        1 cccgcgggcc tgtgcgagtg ggtgcgcgcg cgcgtggcgg cgggcgaggg cggcgaggtg
       61 ccggcgcagt ggacgtcggt ggccggcacc cggcgcctgc gctgcagcct gcatccggcg
      121 agcgccgagg acgagtggct ggtggtgctc accgagagcg accacggcgc ggccgtcgag
      181 gcgctcgcgc tgcgattccg gctcacggcc cgcgaggccg aggtgctgta ctgggtcgcc
      241 cagggcaaga ccaaccgcga catcggcgag atcctcggca cgagcccgaa gacggtcacc
      301 aagcacctcg agcacgtgtt ccgcaagctg ggcgtcgaga cgcgcacggc ggcggcctcg
      361 cgcgtgatcg ggacgctgcc ggagctgctg cggcgctgac gccggctacg acacatgtcg
      421 tattcccgcg cggcgcgggt atgcctatcg tcgagccatc gcctcccgat ccggaggtgc
      481 gatccccgag caacgccagc cacactccca ggagggaccc cgcgatgacc ctttcccgcc
      541 gcctcttcaa cctgtccctg gtcgcgacgg ccgccgccgt cgcgtcgacc ggcgcacagg
      601 ccgccgacac gatcaaggtc ggcatcctgc actcgctgtc cggcacgatg gccatctccg
      661 agacggtgct caaggacacg gcgctcatga ccatcgccga gatcaacgcc aagggcggcg
      721 tgctcggcaa gcagctcgag ccggtggtcg tcgacccggc gtcgaactgg ccgctgttcg
      781 cggagaaggc gcgccagctg atcgcgcagg acaaggtcgc cgtcgtcttc ggctgctgga
      841 cgagcgtgtc gcgcaagtcc gtcctgccgg tcttcgagga gttgaacagc ctgctgttct
      901 acccggtgca gtacgagggc gaggagctct ccaagaacgt gttctacacc ggcgccgcgc
      961 ccaaccagca ggcgatcccg gcggtcgaat acctgatgtc caaggacggc ggctcggcga
     1021 agcgcttcgt gctgctgggt accgactacg tctacccgcg caccaccaac aagatcctgc
     1081 gcgccttcct ccacagcaag ggcgtcgccg acgccgacat catggaggag tacacgccct
     1141 tcggccacag cgactaccag tccatcatcg cgcggatcaa gaagttctcg ggcgagggca
     1201 agaagacggc ggtggtctct accatcaacg gcgactccaa cgtgccgttc tacaaggagc
     1261 tgggcaacca gggcctgaag gcgaccgagg tgccggtcgt cgcgttctcg gtcggcgagg
     1321 aggaactgcg gggcgtcgac accaagccgc tcgtgggcaa cctcgcggcg tggaactact
     1381 tcatgtcgat caagaacccg accaacgccg ccttcatcaa gcaatggtcg gattacgcga
     1441 agaagaacaa cctgcccgcg gacaaggaca agccgctcac caacgacccg atggaggcga
     1501 cctacgtcgg catccacatg tgggcgcagg cggtggagaa ggcgaagtcg accgacaccg
     1561 acaaggtgat cgcggcgatg gccggccaga ccttcaaggc ccccagcggc ttcgtgctgg
     1621 agatggatcc gaagaaccat cacctgcaca agcccgtctt catcggcgag gtgcgcgcgg
     1681 acggccagtt caacgtggtc tggaagacgc ccgcgccgat caaggcgcag ccgtggagtc
     1741 cctacattcc cgaggacaag ggcaagaagg acgagcccga gaagaaatga gcccccacgg
     1801 tcgctagcgc tccgaggctt gcggcaagca tgccgcaagc cgctgcggca gggtctccgc
     1861 agcgccggag ccagtcggca catcgctgcg ctcgcggcag catgaaggaa ccaatgccac
     1921 gcttcaccat cgatgcactg ctgcgagggg tcgcagcgat cgcgctcggc atcgcgttga
     1981 gtcccgcctg ggcgctcgac gcgtcccagg cgcgcgcgat cgccgtcggc gacggcgatg
     2041 cgcgcatcgc ggcgctggac gccgccgtgg agcgcgccga cccgggcctg gcggggttcg
     2101 cgcaggcgct gctggacgac gccgtcaaga cgacggcgac cggcgtgtac gtggtgcgcg
     2161 acgacaaggc cgtcgacgcg gtcaccgggg cgcccgcgac cttgccggat gatgcgcagg
     2221 acgtcaccag caacaaccgc atgcgcggcg cgctgcaggc ggcgctgagc ggtctgaagc
     2281 tgctgtcgaa ggacgccggc cagcgccatg cggcgatcga tgagctgtcc agcgccgatc
     2341 ccgatccggc gctgctgccc gtgctggacc gggcctgggc cgccgagacc gacggccacg
     2401 caaggcgcca gctggacc
//
LOCUS       DASVLN010000757         9842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_149476, whole genome shotgun sequence.
ACCESSION   DASVLN010000757 DASVLN010000000
VERSION     DASVLN010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149476"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VF453_18005"
     CDS             <1..514
                     /locus_tag="VF453_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HEX7689606.1"
                     /translation="LGVRVVRLRLGVVLGREDGAYPMLALAARFALGAVLGSGAQAAP
                     WIHLDDVVGLACRALDDGGLAGAINAVAPDTPVQARFARALAASFGRGVHLRVPHGPM
                     RRLLGEMAELLLEGQHVAPAAALRAGYAFRFPTLESAFADLAVAPGGDPWPKGDGDAA
                     PARAQAGRPR"
     gene            648..1481
                     /gene="panB"
                     /locus_tag="VF453_18010"
     CDS             648..1481
                     /gene="panB"
                     /locus_tag="VF453_18010"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX7689607.1"
                     /translation="MSAHSSPAVASGRAAVTLHRLREMRARGEKIAMLTCYDATFAQV
                     LDEAGVDTMLVGDSLGNVLQGRTSTHGVTLADIAYHTECVARMQPAAWLIADLPFGTY
                     EGGKGVALDASVALLRAGARMVKLEGGDWTAEVVEHLVSRGIPVCGHLGYTPQRVHAL
                     GVRVQGKGEAAAAQLKRDSRALSEAGAAMLVLEMVPAELATEITTGNPELLTIGIGAG
                     GGTSGQVLVLHDMLGLGSGRKPRFVRNFMAEGGSIASAIGRYVAAVKDGSFPQADVHT
                     Y"
     gene            1622..2467
                     /gene="panC"
                     /locus_tag="VF453_18015"
     CDS             1622..2467
                     /gene="panC"
                     /locus_tag="VF453_18015"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX7689608.1"
                     /translation="MQVIHTISDLRAVLAGSTRTALVPTMGNLHEGHLALVREAALHG
                     FPVVATIFVNRLQFLPHEDFDQYPRTLDRDCELLEQAGCDIVFAPSEAELYPEAQAFK
                     VVPPGDIADILEGEFRPGFFTGVCTVVMKLFQCVQPAVAVFGKKDYQQWMVLARMVRQ
                     FAMPVEIVGAPTVRANDGLALSSRNGYLSAEQREEAVALSRALHAAAMAIKAGATEVG
                     AVEQAQLDALSSRGWKPDYVTVRRRADLLPPTPTDLAAKLPLVALAAARIGNTRLIDN
                     LEIDF"
     gene            2541..3569
                     /gene="bioB"
                     /locus_tag="VF453_18020"
     CDS             2541..3569
                     /gene="bioB"
                     /locus_tag="VF453_18020"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX7689609.1"
                     /translation="MNAPVSPIHIQRRAPGAQEACGGHPHVERWSVDDIEALLALPFN
                     DLILRAQAVHREHFVPNQVELATLLSIKTGGCPEDCGYCPQSVHYDTGVEAGKLMDVE
                     AVAAAARTAKEHGATRFCMGAAWRAPKDRDIEAVSAIVREVKSLGLEACCTLGMLTEP
                     QAKSLKDAGLDYYNHNLDTAPEAYGRIISTRDYQDRLDTLGHVRDAGIHVCSGGIVGM
                     GETRRERAGLIAQLANLDPYPESVPINDLVRVEGTPLADQAPLDPLEFVRMIAVARIT
                     MPKARVRLSAGRQQMSEAVQALCFLAGANSVFYGEKLLTTGNPDVEKDKALFEKLGVT
                     TAASQHVG"
     gene            3766..4011
                     /locus_tag="VF453_18025"
     CDS             3766..4011
                     /locus_tag="VF453_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689610.1"
                     /translation="MNRSIARGALAATLLAAAGLALAQQPGTPGTNPNTPQAGTGNAG
                     DNTAQPGSTPSAAHTHHKRHHKHHAKKASAAASAASE"
     gene            complement(4088..4864)
                     /locus_tag="VF453_18030"
     CDS             complement(4088..4864)
                     /locus_tag="VF453_18030"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEX7689611.1"
                     /translation="MSAFNEERVLSVHHWTDRLFSFTTTRDTSLRFSNGHFTMIGLRK
                     EDGKPLLRAYSIVSANYEEHLEFLSIKVPDGPLTSRLQHIQVGDTIIVGRKPTGTLLI
                     DYLTPAKRLYLLSTGTGVAPFLSIIRDPETFERFEQVVLVHGVRQANELAYHDYITEH
                     LPRHEFLGEMISGKLLYYPTVTREAFRNTGRVTTLLESGKLGADLGIPPLNPAEDRVM
                     ICGSPEMLRDLKHMMESRGFKEGNTTTPGDFVIERAFVEQ"
     gene            complement(4974..5921)
                     /locus_tag="VF453_18035"
     CDS             complement(4974..5921)
                     /locus_tag="VF453_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7689612.1"
                     /translation="MRRKLPSTGALEAFEAAARHQSFTKAAEELSVTQSAVCRQVGAL
                     EDFLGVPLFRRTRRGVALTEAGQAYGRTVAARLDDVERDTLELMARGGRGGALELAVV
                     PTFGTRWLLPRLPAFHRAHPDIVVHLSSRTRPFLFADTPFDAAIHAGEAAAADWPGTE
                     RRLLMHEELVAVASPALVRAHGGGAPRDAAGWARLPLLQQSTRPHAWRQFFERLGLAV
                     PQATALAGPRMELFSMLAEAAVQGMGVALTPPWFVREELAQGRLASLSGRPVSGDRAY
                     SLIYPEDKADRPALQAFAAWLQAEATAPGSTMGGNSPTE"
     gene            6070..7251
                     /locus_tag="VF453_18040"
     CDS             6070..7251
                     /locus_tag="VF453_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7689613.1"
                     /translation="MSSSRASFHWDDPLLLAEQLTDDERMVADSARAYCQDKLAPRVL
                     EAFRHERMDVSIFPEMGALGLLGPTIPEQYGGAGLNYVSYGLIAREVERVDSGYRSMM
                     SVQSSLVMVPINEFGNEATKRKYLPKLATGESIGCFGLTEPNHGSDPGSMVTRARKVE
                     GGYALTGNKMWITNSPVADVFVVWAKDDAGAIRGFVLEKGWKGLSAPAIHGKVGLRAS
                     ITGEIVMDEVFCPDENAFPEVRGLKGPFTCLNSARYGIAWGALGAAEDCWHKARQYAL
                     DRKQFGKPLAANQLVQKKLADMQTEITLGLQGCLRLGRMKDEGTAAVEITSIMKRNSC
                     GKALDIARVARDMLGGNGISDEFGVARHLVNLEVVNTYEGTHDIHALILGRAMTGIAA
                     F"
     gene            7322..8536
                     /locus_tag="VF453_18045"
     CDS             7322..8536
                     /locus_tag="VF453_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7689614.1"
                     /translation="MTNAPRALQGVKVLDLSRVLAGPWCGQLLADLGADVVKVERPGA
                     GDDTRSWGPPWLKDAQGEATRDAAYYFCANRNKRSVTIDISTAEGQDLVRRLAMEADV
                     VIENFKVGGLVQYGLDHASLLARKPSLVYCSITGFGQTGPYAQRAGYDFLIQGMGGLM
                     SITGRADGEDGAGPQKVGVALTDILTGLYATVGVLAALAHRDRTGQGQHVDLALLDVQ
                     VACLANQTANYLIGGTVPRRMGNAHPNIVPYQDFPTADGDMILAVGNDGQFAKLCEVI
                     GRPEWSRDDRFATNAGRVKHRAELIPLLRQATVMRTTREWIAALEGAGVPCGPINRLD
                     EVFSDPQVVARGLRIDLPHAVAGTVPGVANPIRMSASPVAYHRGPPVLGEHTQEVLGQ
                     WLGMAGVDAAAR"
     gene            complement(8591..8812)
                     /locus_tag="VF453_18050"
     CDS             complement(8591..8812)
                     /locus_tag="VF453_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX7689615.1"
                     /translation="MSGSALPPGLDRPERDVLCPTCRQPCKYGPSNPSRPFCSPRCRN
                     ADFGAWANEAYRVPAASDPGDEEDGANPA"
     gene            complement(8809..9564)
                     /gene="zapD"
                     /locus_tag="VF453_18055"
     CDS             complement(8809..9564)
                     /gene="zapD"
                     /locus_tag="VF453_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEX7689616.1"
                     /translation="MVLYEYPFNEGIRTMLRLEHLFDRMAGLIARDDPVDHHYALASM
                     FEIMDVAARADLKSDVLKELDRHRSQLQSYRGNPAISEQVLDTVVERIDAAFNALNES
                     PGKAGQALTANEWLMSIRSRISIPGGTCAFDLPAYYAWQQYPAEHRRADLQRWTDTLS
                     ALSSSLHLLLGLLRESGVPHKVVATGGQYQQSLPATRTYHLLRVKLPHEADLVPEISG
                     HRLMVSIRLQKADAEGRLRPWTDDLAFEMALCS"
BASE COUNT         1432 a         3457 c         3562 g         1391 t
ORIGIN      
        1 gctgggtgtg cgcgtggtgc ggctgcgcct gggcgtggtg ctcgggcggg aggacggcgc
       61 atatccgatg ctggcgctgg ccgcgagatt cgcgctgggc gcggtgctcg gctcgggcgc
      121 gcaggcggcg ccgtggatcc atctcgacga cgtcgtcgga cttgcgtgcc gcgcgcttga
      181 cgacggtggg ctcgccggcg cgatcaacgc cgttgcgccg gacacacccg tccaggcccg
      241 cttcgcgcgc gcgctggcgg cctcgttcgg acgcggcgtg cacctgcgcg tgccgcatgg
      301 gccgatgcgg cgcctgctcg gtgagatggc ggagctgctg ctcgagggcc agcacgtcgc
      361 gccggccgca gccctgcgcg cgggctatgc cttccgcttc ccgacgctgg agtcggcgtt
      421 cgccgacctg gcggtcgcgc ccggcggcga cccgtggccc aagggcgacg gcgacgcggc
      481 tcccgcacgg gcgcaggcgg gccgtccacg ctagagacct gtccccaaca aaccacatcg
      541 acgcgcgcgg ccgccggccg cgtgtgcaca atgccgcctc cgccgtgtcc gccgcccgat
      601 cgcgggtggc gccgcggccg tcccgtcgca gatccaaagg actcgccatg agcgcccact
      661 ccagccccgc cgtcgcctcc ggccgcgccg cggtcaccct gcatcgcctt cgcgagatgc
      721 gcgcgcgcgg cgagaagatc gcgatgctca cctgctacga cgcgaccttc gcgcaagtgc
      781 tggacgaagc gggcgtcgac acgatgctgg tgggcgactc gctcggcaac gtgctgcagg
      841 ggcgcaccag cacgcacggc gtgacgctgg ccgacatcgc gtaccacacc gagtgcgtgg
      901 cgcgcatgca gccggcggct tggctcatcg ccgacctgcc gttcggcacc tacgagggcg
      961 gcaagggcgt ggcgctcgac gcgtccgtgg cgctgctgcg cgccggtgcg cgcatggtca
     1021 agctcgaggg cggcgactgg acggccgagg tcgtcgagca cctggtctcg cgcggcatcc
     1081 cggtctgcgg tcacctcggc tacaccccgc agcgcgtgca cgcgctgggc gtgcgcgtgc
     1141 agggcaaggg cgaggcggcc gcggcgcagc tgaagcgcga ctcgcgcgcg ctgtccgagg
     1201 ccggcgccgc gatgctggtg ctggagatgg tgccggccga gctggccacc gagatcacga
     1261 ccgggaatcc cgagctgctg acgatcggca tcggcgccgg cggcggcacc tccggccagg
     1321 tgctggtgct gcacgacatg ctgggcctgg gctccggccg caagccgcgc ttcgtgcgca
     1381 atttcatggc cgaaggcggc tccatcgcgt cggccatcgg gcgctacgtc gcggcggtca
     1441 aggacggcag cttcccgcag gctgacgtcc acacctactg aaatcggcgc tgccgcggga
     1501 cggccggcgc caaatcgggg gtttccgggc cgttgcgcac gcgcggcccc cggcccgcgg
     1561 cgaacctgcg acactcgcgc ccatcgacgg ccacccggcc cgtccattgc cgcccgaagc
     1621 catgcaagtc atccacacga tctccgacct ccgcgccgtc ctcgccggct cgacgcggac
     1681 cgcgctcgtc ccgaccatgg ggaacctgca cgagggccat ctcgcgctgg tgcgcgaggc
     1741 ggcgctgcac ggcttcccgg tggtcgcgac catcttcgtc aatcgcctgc agttcctgcc
     1801 gcacgaggac ttcgaccagt atccgcgcac gctggatcgg gactgcgagc tgctcgagca
     1861 ggcgggctgc gacatcgtct tcgcgccgag cgaggccgag ctctatcccg aggcgcaggc
     1921 gttcaaggtc gtgcccccgg gcgacatcgc cgacatcctg gagggcgagt tccgccccgg
     1981 cttcttcacc ggcgtgtgca cggtggtgat gaagctgttc cagtgcgtgc agccggcggt
     2041 cgccgtcttc ggcaagaagg actaccagca gtggatggtg ctcgcgcgca tggtgcgcca
     2101 gttcgcgatg cccgtcgaga tcgtcggcgc tcccaccgtg cgcgcaaacg acgggctcgc
     2161 gctgtcctcg cgcaacggct acctgagcgc cgagcagcgc gaggaggccg tcgccctctc
     2221 gcgcgcgctg cacgcggcgg cgatggcgat caaggcgggc gcgacggagg tcggggcggt
     2281 ggagcaggca cagctcgacg cgctgtcatc gcgcggctgg aagccggact acgtcacggt
     2341 ccgccgccgc gccgacctgc tgccgccgac acccaccgac ctggcggcca agctgccgct
     2401 ggtggcgctg gccgcggccc gcatcggcaa cacgcggctc atcgacaacc tcgagatcga
     2461 tttctgatcc gtcgccgcgg atccccgagc gcggcggacg gtcaagccgg cgcgccggat
     2521 cggtggtgca aaatcccggg atgaacgcac cagtctcccc gatccacatc cagcgtcgcg
     2581 ccccgggagc gcaagaggcc tgcggcggcc atccgcacgt cgagcgctgg tccgtggacg
     2641 acatcgaggc gctcctcgcg ctgcccttca acgacctgat cctgcgcgcc caggccgtgc
     2701 accgcgagca cttcgtgccc aaccaggtcg agctcgcgac actgctgtcg atcaagaccg
     2761 gcggctgccc cgaggactgc ggctactgcc cgcagtcggt gcactacgac acgggcgtcg
     2821 aggcgggcaa gctgatggac gtcgaagccg tcgccgcggc ggcgcggacg gccaaggaac
     2881 acggcgcgac ccgcttctgc atgggcgccg cgtggcgcgc gcccaaggac cgtgacatcg
     2941 aggccgtctc ggccatcgtg cgggaggtga agtcgctcgg cctggaagct tgctgcacgc
     3001 tgggcatgct gaccgagccg caggccaagt cgctcaagga cgccggcctc gactactaca
     3061 accacaacct cgacacggcg cccgaggcct acggccgcat catctcgacg cgcgactacc
     3121 aggatcgcct cgacacgctc ggccacgtcc gcgacgccgg catccatgtc tgctcgggcg
     3181 gcatcgtggg catgggcgag acgcggcgcg agcgtgccgg actgatcgcg cagctggcca
     3241 acctcgaccc gtaccccgag tccgtgccga tcaacgacct ggtgcgcgtc gagggtacgc
     3301 cgctggccga tcaggcgccg ctcgacccgc tcgaattcgt gcgcatgatc gcggtcgccc
     3361 gcatcaccat gcccaaggcg cgcgtgcgcc tgtcggccgg ccgccagcag atgagcgagg
     3421 ccgtgcaggc gctgtgcttc cttgccggcg cgaactccgt cttctacggc gagaagctgc
     3481 tgacgaccgg caacccggac gtcgagaagg acaaggccct gttcgagaag ctgggcgtca
     3541 cgacggcggc cagccagcac gtgggctgac ggcctcgcgc gcagacgcat cacgcgcgcc
     3601 cgcgcgtgcg atcctcatca cggcccgcac gcagcgcgtt gcgggccgtc ttcattccgg
     3661 gcgccgaggc cgatgccggc gccgcgccgc tcggcggggc atcccgcttg ctcggctcgc
     3721 gcggcccgcg atgtccgcgg gcgacgactt ccaaggagct ccctcatgaa tcgttccatc
     3781 gcgcgcggtg ccctggccgc gacgctgctg gccgccgccg gcctggcgct ggcgcagcag
     3841 cccggcaccc ccggcaccaa cccgaacacc ccgcaggcgg gcacgggcaa cgccggcgac
     3901 aacaccgcgc agcccggctc gacgccgtcg gcggcgcaca cgcaccacaa gcgccatcac
     3961 aagcatcacg cgaagaaggc cagcgcggcc gccagcgcgg caagcgagtg aagacaggcg
     4021 tcgcgccgcg gacaggcggt gcgacgctcc cgcgcgggcg cgcctcacgc gcgcccgcgc
     4081 ggcctcgtca ttgctcgacg aacgcgcgct cgatgacgaa atcgcccggc gtcgtcgtgt
     4141 tgccttcctt gaacccgcgc gactccatca tgtgcttgag gtcgcgcagc atctcggggc
     4201 tgccgcagat catcacgcga tcctcggccg ggttcagcgg cgggatgccc aggtcggcgc
     4261 cgagcttgcc ggactccagc agcgtcgtga cgcggccggt gttgcggaag gcctcgcgcg
     4321 tgacggtggg gtagtacagc agcttgccgc tgatcatctc gccgaggaac tcgtgcctgg
     4381 gcaggtgctc ggtgatgtag tcgtggtagg ccagctcgtt ggcctggcgc acgccgtgca
     4441 cgaggacgac ctgctcgaag cgctcgaagg tctccgggtc gcggatgatg ctcaggaagg
     4501 gcgcgacgcc ggtgccggtg gacagcaggt acaggcgctt ggccggcgtc aggtagtcga
     4561 tcagcagcgt gcccgtgggc ttgcggccga cgatgattgt gtcgccgacc tggatgtgct
     4621 gcaggcgcga ggtcagcggg ccgtcgggca ccttgatcga caggaactcc aggtgttcct
     4681 cgtagttcgc gctcacgatg gaataggcgc gcagcagcgg cttgccgtcc tccttgcgca
     4741 ggccgatcat cgtgaagtgg ccgttcgaga agcgcagcga ggtgtcccgc gtggtggtga
     4801 agctgaacag ccggtcggtc cagtgatgca cgctcagcac gcgctcttcg ttgaaggcac
     4861 tcatggtcgg ggcgggggtc ggtcggggat gcggggaagc cgcagcggga aggtgtcccc
     4921 ttctgcgaac ccgtgattgt cgcagaccag gcatctgcgg ataacgcata agctcattcg
     4981 gtcggggaat tgccgcccat cgtggatccg ggggccgtgg cctcggcctg cagccaggcg
     5041 gcgaaggcct gcagggccgg ccggtcggcc ttgtcctcgg ggtagatcag cgagtaggcg
     5101 cggtcgccgc tcacgggccg gcccgagagg ctggcgagcc ggccctgcgc cagctcctcg
     5161 cgcacgaacc agggcggcgt cagcgcgacg cccatgccct gcaccgcggc ctcggcgagc
     5221 atcgagaaca gctccatccg cggccccgcc agcgccgtcg cctgcggtac ggccagcccg
     5281 aggcgctcga agaactggcg ccaggcgtgc ggccgggtgg attgctgcag cagcggcagg
     5341 cgtgcccagc cggcggcgtc gcgtggcgcg ccgccgccgt gggcgcgcac gagcgcgggg
     5401 ctcgcgaccg ccacgagttc ctcgtgcatc aggaggcgtc gctcggtgcc gggccagtcg
     5461 gcggccgccg cctcgcccgc gtggatcgcg gcgtcgaacg gcgtgtcggc gaagaggaag
     5521 gggcgggtgc gcgagctcag gtgcacgacg atgtccggat gcgcgcggtg gaacgccggc
     5581 agccgcggca gcagccagcg agtgccgaag gtcggcacga cggccagctc gagcgcgccg
     5641 ccgcgcccgc cgcgggccat cagctcgagc gtgtcgcgct cgacgtcgtc cagtcgcgcc
     5701 gccacggtgc ggccgtacgc ctgccccgcc tcggtcagcg cgacgccgcg ccgcgtgcgc
     5761 cggaacagcg gcacgccgag gaagtcctcc agcgccccga cctggcggca cacggcgctc
     5821 tgcgtgaccg acagctcctc ggccgccttg gtgaagctct ggtgccgggc ggcggcctcg
     5881 aaggcctcca gcgcgccggt ggagggcagc ttgcgtcgca tgtgcgatcg gtcacgcttg
     5941 tttcggagtg cgtattgtgc acacgtccgt gcggataaag cgtttgtttc gccaggagag
     6001 ccgccctata gtgccgcgat cgtgcgaaac gcgcacatga accgtttttc cgtcgagaaa
     6061 gtgcccgcca tgagctcctc ccgcgccagc ttccactggg acgatccgct gctgctcgcc
     6121 gagcagctga ccgacgacga gcgcatggtc gccgactcgg cgcgcgccta ctgccaggac
     6181 aagctcgcgc cgcgggtact ggaggcgttc cgccacgagc gcatggacgt gtccatcttt
     6241 cccgagatgg gcgcgctggg cctgctcggc ccgacgattc ccgagcagta cggcggcgcc
     6301 gggctgaact acgtcagcta cggcctgatc gcgcgcgagg tcgagcgcgt ggactccggc
     6361 taccgctcga tgatgagcgt gcagagctca ctcgtgatgg tgcccatcaa cgagttcggc
     6421 aacgaggcga ccaagcgcaa gtacctgccg aagctggcga caggcgagtc gatcggctgc
     6481 ttcggcctga ccgagcccaa ccacggttcg gatccgggca gcatggtcac gcgcgcgcgc
     6541 aaggtcgagg gcggctatgc gctcaccggc aacaagatgt ggatcacgaa ctcgccggtg
     6601 gccgacgtgt tcgtcgtctg ggccaaggac gacgctggcg cgatccgcgg cttcgtgctc
     6661 gagaagggct ggaagggcct gtcggcgccg gcgatccacg gcaaggtggg actgcgcgcg
     6721 tccatcaccg gcgagatcgt gatggacgag gtcttctgcc ccgacgagaa cgccttcccc
     6781 gaggtgcgcg ggctcaaggg gccgttcacc tgcctgaact cggcgcgcta cggcatcgcc
     6841 tggggcgcgc tgggcgcggc cgaggactgc tggcacaagg cgcgccagta cgcgctcgac
     6901 cgcaagcagt tcggcaagcc gctcgcggcc aaccagctgg tgcagaagaa gctcgccgac
     6961 atgcagaccg agatcacgct gggcctgcag ggctgcctgc gcctgggccg catgaaggac
     7021 gagggcacgg cggcggtcga gatcacgtcg atcatgaagc gcaattcctg cggcaaggcg
     7081 ctcgacatcg cccgcgtcgc gcgcgacatg ctgggcggca acggcatctc cgacgagttc
     7141 ggcgtcgcgc gccacctggt caacctcgag gtggtcaata cctacgaggg tacgcatgac
     7201 atccacgcgc tgatcctggg ccgtgcgatg accggaatcg ccgcattttg agcccctgcc
     7261 ggcgagcggc agcgccgggg ccggtggcac gccagccagt cccaatgatc ggagtgtcag
     7321 catgacaaat gcaccgcgcg cgctgcaggg cgtgaaggtg ttggatctct cgcgcgtgct
     7381 cgcgggcccg tggtgcggcc agctgctggc cgacctcggc gcggacgtcg tgaaggtcga
     7441 gcgcccgggt gccggcgacg acacgcgctc gtggggcccg ccctggctga aggacgcgca
     7501 gggcgaggcg acgcgcgacg cggcctacta cttctgcgcc aaccgcaaca agcgctcggt
     7561 caccatcgac atctcgaccg ccgaaggcca ggacctcgtg cgccggctcg cgatggaggc
     7621 cgacgtggtg atcgagaact tcaaggtcgg cgggctggtg cagtacggcc tggaccacgc
     7681 gagcctgctg gcgcggaagc cgtcgctggt ctactgctcc atcacgggct tcggccagac
     7741 cggcccctac gcgcagcgcg ccggctacga cttcctgatc cagggcatgg gcgggctcat
     7801 gagcatcacc ggccgcgccg acggcgagga cggcgcgggc ccgcagaagg tgggcgtggc
     7861 gctgaccgac atcctcaccg gcctgtacgc gaccgtcggc gtgctggcgg cgctcgcgca
     7921 ccgcgatcgc accggccaag gccagcacgt cgacctcgcg ttgctggacg tccaggtcgc
     7981 ctgcctcgcc aaccagaccg ccaactacct gatcggcggc acggtgccgc gccgcatggg
     8041 caacgcgcat cccaatatcg tgccctacca ggacttcccg acggccgacg gcgacatgat
     8101 cctggcggtc ggcaacgacg gccagttcgc caagctgtgc gaggtgatcg gccggcccga
     8161 gtggtcgcgc gacgatcgct tcgccaccaa cgcgggccgc gtgaagcatc gcgccgagct
     8221 gatcccgctg ctgcgccagg ccaccgtgat gcgcacgacg cgcgagtgga tcgccgcgct
     8281 cgagggcgcc ggcgtgccct gcgggcccat caaccggctg gacgaggtct tctccgatcc
     8341 gcaggtggtc gcgcgcggcc tgcgcatcga cctgccgcac gcggtcgccg gcacggtgcc
     8401 gggcgtcgcg aacccgatcc ggatgtcggc gtcgccggtg gcctaccacc ggggcccgcc
     8461 ggtgctgggc gagcacacgc aggaggtgct cggccaatgg ctggggatgg cgggcgtgga
     8521 cgccgcggcg cgctgagcgg cgcgcgcttg cacgcgtcag gcgcgcgcaa ggcgccctgt
     8581 cgggagcgtg tcaggcgggg ttcgcgccgt cttcctcgtc gcccggatcg ctcgccgccg
     8641 gcacgcggta ggcctcgttg gcccaggcgc cgaagtcggc gttgcggcag cgcgggctgc
     8701 agaacgggcg cgacgggttg gacggcccgt acttgcaagg ctggcggcag gtcgggcaga
     8761 ggacgtcgcg ttcgggccgg tcgaggccgg gcggcagggc ggatccgctc atgagcacag
     8821 cgccatctcg aacgcgaggt cgtccgtcca gggccgcagc cgcccctcgg catcggcctt
     8881 ctgcaggcgg atggagacca tcaggcgatg gccgctgatc tccggcacga ggtcggcctc
     8941 gtgcggcagc ttcacgcgca gcaggtgata ggtgcgcgtg gccggcaggc tctgctggta
     9001 ctggccgccg gtggcgacga ccttgtgcgg cacgcccgac tcgcgcagca gtcccagcag
     9061 caggtgcagc gacgacgaca gggcgctcag cgtgtccgtc cagcgctgga ggtcggcgcg
     9121 gcgatgctct gcgggatatt gctgccaggc gtagtaggcc ggcaggtcga aggcacaggt
     9181 gccgcccgga atgctgatgc ggctgcggat gctcatcagc cactcgttgg cggtcagcgc
     9241 ctggccggcc ttgccgggcg attcgttgag cgcgttgaag gcggcatcga tgcgctcgac
     9301 caccgtgtcg agcacctgct cggagatcgc cggattgccg cggtagctct gcagctgcga
     9361 gcgatggcga tcgagctcct tgaggacgtc ggacttcagg tcggcgcgcg cggcgacgtc
     9421 catgatctcg aacatggacg cgagcgcata gtggtggtcg accggatcgt cgcgcgcgat
     9481 gaggccggcc atgcggtcga acaggtgttc gaggcgaagc atcgtgcgga tgccttcgtt
     9541 gaacgggtat tcgtagagga ccaaggcgtt ctcggattgc ggctgcgctg gaatgcgcgt
     9601 aagcgcgatt gtttcacacg cgttaccccg aagccttccc ccgaaggacg gtcgcgcgca
     9661 tttgcagcct tgtcctacaa aaccgcacga cggtgtggcg ggcgcgacgc gccgcccggc
     9721 cggcggcgaa ccgcggactc aggcgccgct tgcgccccag gcgtcccaca gccggtcgag
     9781 cgcggcgccg aaggccgcca gcgacagccg gtcgtcgttg aagaccaccg catcggcgat
     9841 cg
//
LOCUS       DASVLN010000758         6279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_254254, whole genome shotgun sequence.
ACCESSION   DASVLN010000758 DASVLN010000000
VERSION     DASVLN010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254254"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VF453_18060"
     CDS             <1..694
                     /locus_tag="VF453_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEX7689617.1"
                     /translation="ARGLEALRALPPGVARRTQALRLKLQAARMASKPVEALQTARLL
                     AKHQGFSQLAAQSLLRSLAIDVVCAAHDEEQLQRAWLSLEPADRRDVVVGARAAVRAC
                     ELGRADLGRDYLLPHWAELAKMEVDQRATLAIALVDCIAGLGNDLLPRVEAAAVAYGH
                     EPAVVAAAGMVFAERQLWGKARRPLEQTAQNTALPGRVRRRALRRLAFMARLEGDESR
                     GGELERAAAAID"
     gene            757..833
                     /locus_tag="VF453_18065"
     tRNA            757..833
                     /locus_tag="VF453_18065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:791..793,aa:Arg,seq:acg)
     gene            971..1047
                     /locus_tag="VF453_18070"
     tRNA            971..1047
                     /locus_tag="VF453_18070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1005..1007,aa:Arg,seq:acg)
     gene            1216..1944
                     /locus_tag="VF453_18075"
     CDS             1216..1944
                     /locus_tag="VF453_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689618.1"
                     /translation="MDGEPTSGRDSVFVARGISKTYRTGEVEVRALRDVDLEIRRGEF
                     VVLLGPSGSGKSTLLNILGGLDVPTSGELRFGAQRLDAASDAALTAYRREHVGFVFQF
                     YNLIPSLTVRENVALVTDIVDAPMPVDEAIDLVGLTPRRDHFPAQLSGGEQQRVAIAR
                     AIVKKPEVLLCDEPTGALDYQTGKLVLEVIARINRELGTTAIVITHNAAIAGMADRVV
                     TLADGRIQRVEPNARKRTPAELSW"
     gene            1948..4317
                     /locus_tag="VF453_18080"
     CDS             1948..4317
                     /locus_tag="VF453_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7689619.1"
                     /translation="MKALDRKLLRDLRLLWSQALTIALVVASGIGGFVTSLSAVDSLA
                     LARDDFYAAGRFADMFAGVKRAPDALADALRDVPGVADVQTTVQAIVRVEIPGLPDPI
                     IGQVIGLDARHPSRMNLVSVSAGQALQGAGERADGSIPALVSEGFAQARHLRPGDTLD
                     ALMNGKRRTLRMVGTALSPEFIFAGLWGMPDMRGYGIFWIDRDTLAAAYDMDGAFNQV
                     AVKLAPGASERAVAEGLGRRLAAYGGRDVIGRADQASNQMLDNEIKEQRVLGTVLPTI
                     FLGVAAFLLNVVVSRLVGTQREQIAALKALGYPDGTIARHYLKLVLAIVLAGLALGIV
                     VGDRLGAMFTGLYAEFFHFPRFEHRIAPWLLLTSLGITVATAVLGTLNAILRTVRLPP
                     AEAMRPPAPGRFGPTLLERLGIRAMGPALRMIVRNMERRPLRTTLGIGGIAAAVAITI
                     MGNFLRDAIDVVVDTQFTLSLRGDVSLWTYEPVDDVGRLELARLPGVTQVESTRFVPV
                     LLVHGHRRERSLLRGYAPRAELYRVIDMDRRQTLLEGRGLLLTDRLADKLGVRAGDTV
                     VVEVLAGKARTLALTVSGTVREMMGLNAYLDRDTLDRLLGDGDVSTGWVLSVERGREA
                     ALLEASKALPQVAGAFSKATMLRNMQEISARNVRIMSTILTAFAAVISVGVVYNNARI
                     ALSERGWELASLRVLGFTRVEVSVLLLGEMALSIAIALPLGMLLGWGLVHGVSELLKS
                     DQFFFPVAIQPRTYAWAALCVLVAGAGSGLVVRRRVDRLDIVAVLKTRE"
     gene            4319..5515
                     /locus_tag="VF453_18085"
     CDS             4319..5515
                     /locus_tag="VF453_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7689620.1"
                     /translation="MRKTSWMAAAAAASAALALLAWAFAPRPVEVEVATVTRGHFETT
                     IDEDGRTRVRDRYVVSAPLAGVVTRIRLREGDTVRAGDPVATLTPTLPPMLDERTLRA
                     QEVRVDIAEANVQRAQARIEGARVALQQADNEAARSERLAGAGFVAPTKLDTDRLAVL
                     AARKELDAAVQEHHASLHEVEEARAAVAAVTRPREGRTFVLQAPVSGRVLRVDQASEA
                     SVQLGAALLELGDVSRIEVVAELLTTDALQARPGSRARIERWGGAGTLEGRVRLVEPE
                     AFTKVSALGVEEQRVRVLIDIASPAEQWAALGDGYRVSVRIVTRALDDALKVPVSAVF
                     PRADGAGMAVFVIDRGRARLVPVELGARNGAEGWVLRGLQAGAQVIVYPPVGVGEGAR
                     VRARRV"
     gene            5561..>6279
                     /locus_tag="VF453_18090"
     CDS             5561..>6279
                     /locus_tag="VF453_18090"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX7689621.1"
                     /translation="MAANDTLGVWIPLSAAVGIGLLVGLERERRKALGPRRIQAGIRT
                     FTLVSLLGAACASAQADWILPAMVVALSALMATAYACSTAPDAGITTGAALLLTLALG
                     ALAMREPEVAVSIGVVATIVLAARARLHDFVRRTITDEELRDGLVLAAAALVILPFIP
                     DRYVGPMQAFNPRTTWIIVVLIMGAGAMGHVAQRMLGATWGLAAAGFAAGFVSSLATV
                     GTMGARARQVPAAARAAVSGA"
BASE COUNT          829 a         2230 c         2313 g          907 t
ORIGIN      
        1 cgcgcgcggc ctggaggcgc tgcgcgcgct gccgccgggc gtggcgcgcc gcacccaggc
       61 gctgcggctg aagctgcagg ccgcgcgcat ggccagcaag ccggtggagg cgttgcagac
      121 ggcccgcctg ctcgccaagc accagggctt ctcgcagctc gccgcgcagt cgctgctgcg
      181 ctcgctggcg atcgacgtgg tctgcgccgc gcacgacgag gagcagctgc agcgcgcctg
      241 gctctcgctc gagcccgcgg atcggcgcga cgtggtggtc ggtgcccgcg ccgccgtgcg
      301 cgcatgcgag ctgggccgcg ccgacctcgg ccgcgactac ctgctgccgc actgggccga
      361 gctcgcgaag atggaagtcg accagcgcgc cacgctcgcg atcgcgctcg tcgactgcat
      421 cgccggcctg ggcaacgact tgctgccgcg cgtcgaggcc gcggcggtcg cctacggcca
      481 cgagccggcg gtcgtcgcgg cggccggcat ggtcttcgcc gaacgccagc tgtggggcaa
      541 ggcgcgccgt ccgctggagc agaccgccca gaacacggcg ctgcccggcc gcgtccgccg
      601 ccgcgcactg cggcgcctgg ccttcatggc ccgtctcgag ggcgacgaga gccgcggcgg
      661 cgaactcgag cgcgcggccg cggcgatcga ctgattccga cttgactgct cagtctttcg
      721 acgaaaattc atcaccatgc tatagtgcaa ggcttcgcgg ctgtagctca gctggataga
      781 gtacttggct acgaaccaag gggtcgtggg ttcgattcct gccagccgca ccaaaaagcg
      841 acgaacgggt catctcgcaa gaggtggccc gtttttcctt gcgcgcgccg cttgcgctcg
      901 tgtggcgctt cagatttttt ttgccgcaag cgtccgtcga aggaattgtc ggtctataat
      961 ccgaggcttc gcggctgtag ctcagctgga tagagtactt ggctacgaac caaggggtcg
     1021 tgggttcgat tcctgccagc cgcaccacag aacaaccgat cgttcagtga ttcgagacgg
     1081 tccaagcgtg aaagcgcttg ggccgttttt ctttgtcgat ctccgccgcc gggacggtcg
     1141 gcgggcccgg gctgccgcgc tccttgcgca agctcaaggg tcgcagcgcc gtcgatcccg
     1201 acactgcgga cggtcatgga cggcgagccc acatccggcc gcgacagcgt cttcgtcgca
     1261 cgcggcatca gcaagaccta tcgcaccggc gaggtcgagg tgcgggcgct gcgcgacgtc
     1321 gatctcgaga tccgccgcgg cgagttcgtc gtgctgctgg ggccctcggg cagcgggaag
     1381 tccacgctgc tcaacatcct gggcggactc gacgtcccga cctcgggcga actgcgcttc
     1441 ggcgcccagc ggctcgacgc cgcctcggac gcggcgctga ccgcctaccg gcgcgagcac
     1501 gtgggcttcg tcttccagtt ctacaacctg atcccgagcc tcacggtgcg cgagaacgtc
     1561 gcgctggtca ccgacatcgt ggacgccccg atgccggtgg acgaggcgat cgacctggtc
     1621 ggactcacgc cgcgccgcga ccacttcccg gcgcagctgt ccggcggcga gcagcagcgc
     1681 gtggcgatcg cgcgcgcgat cgtgaagaag cccgaggtcc tcctgtgcga cgagcccacg
     1741 ggcgcgctgg actaccagac cggcaagctg gtgctggaag tcatcgcgag gatcaaccgc
     1801 gagctgggca ccacggcgat cgtgatcacc cacaacgccg cgatcgccgg catggcggac
     1861 cgcgtggtga ccctcgcgga cggccgcatc cagcgtgtcg agcccaacgc gcgcaagcgc
     1921 acgccggcgg aattgagctg gtaggccatg aaggccctcg accgcaagct gctgcgcgac
     1981 ctgcgcctcc tctggagcca ggcgttgacc atcgcgctcg tcgtcgcgag tgggatcggc
     2041 ggcttcgtca ccagcctgtc ggccgtcgat tcgctggcgc tggcgcgcga cgacttctac
     2101 gcggccggcc gcttcgccga catgttcgcc ggggtcaagc gcgcccccga cgcgctggcc
     2161 gacgccctgc gcgacgtgcc gggcgtcgcc gacgtgcaga cgaccgtgca ggccatcgtg
     2221 cgcgtcgaga tcccggggct gcccgatccc atcatcggcc aggtgatcgg cctcgatgcc
     2281 aggcacccgt cccgaatgaa cctggtcagc gtcagcgccg gccaggcgct gcagggcgcg
     2341 ggcgagcgcg ccgacggcag catcccggcc ctggtctccg agggattcgc gcaggcgcgc
     2401 cacctgcggc ccggcgacac cctggatgcc ctgatgaacg gcaagcgccg gacgctgcgg
     2461 atggtgggca cggccctgtc gccggagttc atcttcgccg ggctctgggg catgcccgac
     2521 atgcgcggct acggcatctt ctggatcgac cgcgacaccc tggcggccgc ctacgacatg
     2581 gatggcgcct tcaaccaggt ggcggtcaag ctggcgccgg gcgcgtccga gcgcgcggtc
     2641 gccgagggcc tcggcaggcg gctcgcggcc tacggcggac gcgacgtcat cggccgcgcg
     2701 gaccaggcct ccaaccagat gctggacaac gagatcaagg agcagcgcgt gctcggaacc
     2761 gtgctgccga cgatcttcct cggcgtcgcg gccttcctgc tcaacgtcgt cgtctcgcgg
     2821 ctggtgggca cgcagcgcga gcagatcgcg gcgctcaagg cgctgggcta tcccgacggc
     2881 accatcgcgc ggcactacct gaagctcgtg ctggccatcg tgctggcggg gctcgcgctg
     2941 ggcatcgtgg tgggcgaccg gctgggcgcc atgttcaccg gcctgtacgc ggagttcttc
     3001 cacttcccgc gcttcgagca ccgcatcgcg ccgtggctgc ttctcaccag cctgggcatc
     3061 acggtggcga ccgcggtgct gggcacgctg aacgcgatcc tgcgcaccgt gcggctgccg
     3121 cccgccgagg ccatgcgtcc gcccgcgccg ggacgcttcg gcccgacgct gctcgagcgc
     3181 ctgggcatcc gggcgatggg cccggcgctg cgcatgatcg tgcgcaacat ggagcgccgg
     3241 ccgctgcgga ccacgctggg catcggcggg atcgcggctg ccgtggcgat cacgatcatg
     3301 ggcaacttcc tgcgcgacgc gatcgacgtg gtggtggaca cccagttcac gctctcgctg
     3361 cgcggcgacg tctcgttgtg gacgtacgag cccgtggacg acgtcggccg cctcgaactg
     3421 gcgcgccttc cgggcgtcac gcaggtcgaa tccacgcgct tcgtgccggt cctgctggta
     3481 cacggacacc ggcgcgagag aagcctgctg cgcggctacg cgccgcgcgc ggagctctac
     3541 cgcgtgatcg acatggaccg ccgccagacg ctgctggagg gccgcgggct gctcctcacg
     3601 gaccgcctgg ccgacaagct gggcgtgcgc gccggcgaca cggtggtcgt cgaggtgctc
     3661 gccggcaagg cgcgcacgct tgccctgacg gtgtcgggca cggtgcgcga gatgatgggc
     3721 ctgaacgcct acctcgatcg cgacacgctc gaccgcctgc tcggcgacgg cgacgtgagc
     3781 acgggctggg tgctgtcggt cgagcgcggc cgggaagcgg cgctcctcga ggcctccaag
     3841 gcgctgccgc aggtcgccgg cgcgttcagc aaggcgacta tgctgcgcaa catgcaggag
     3901 atcagcgccc gcaacgtacg catcatgagc accatcctga ccgcgttcgc cgcggtgatc
     3961 tcggtcggcg tcgtctacaa caatgcgcgc atcgccctgt ccgaacgcgg ctgggagctg
     4021 gcgagcctgc gggtgctcgg gttcacgcgc gtcgaggtgt cggtgctgct gctgggcgag
     4081 atggcgctgt cgatcgcgat cgccctgccg ctggggatgc tgctcggctg ggggctggtg
     4141 cacggcgtga gcgagctgct gaagtcggac cagttcttct tcccggtggc gatccagccg
     4201 cgcacctacg cctgggccgc gctgtgcgtg ctcgtggcag gggccggaag cgggctcgtc
     4261 gtccggcgcc gcgtcgaccg gctcgacatc gtggccgtgc tgaagacgag ggaatgagat
     4321 gaggaagacc tcctggatgg ccgccgccgc ggcggcctcg gcggcgctgg cgctgctcgc
     4381 ctgggcgttc gcgccgcgac cggtcgaggt ggaggtggcc accgtcaccc ggggccactt
     4441 cgagaccacg atcgacgagg acggcaggac ccgcgtgcgc gatcgctacg tggtctctgc
     4501 gccgctggcc ggcgtggtga cgcggatccg gctgcgcgag ggcgacaccg tgcgcgcggg
     4561 cgacccggtc gccacgctga ccccgacgct gccgcccatg ctcgacgaac gcacgctgcg
     4621 cgcccaggag gtgcgcgtgg acatcgccga ggccaacgtc cagcgcgcgc aggcgcgcat
     4681 cgagggcgcg cgcgtcgcgc tccagcaagc cgacaacgag gccgcgcgca gcgaacggct
     4741 cgccggcgcg ggcttcgtgg cgccgaccaa gctggacacg gaccggctcg ccgtgctggc
     4801 ggcgcgcaag gaactggacg ccgccgtcca ggagcatcac gcctcgctgc acgaggtcga
     4861 ggaagcccgc gccgccgtcg cggccgtgac gcgaccgcgc gagggccgca cgttcgtcct
     4921 gcaggcgccg gtgagcgggc gcgtgctgcg cgtcgaccag gccagcgagg ccagcgtgca
     4981 gctcggggcg gccttgctgg agctgggcga cgtctcgcgc atcgaggtcg tcgccgagct
     5041 cctgaccacc gacgcgctgc aggcgcggcc cggcagccgc gcgcggatcg aacgctgggg
     5101 cggcgccggc acgctcgaag gccgcgtgcg cctggtcgag ccggaggcgt tcacgaaggt
     5161 ctcggcgctg ggcgtcgagg agcagcgcgt gcgcgtgctg atcgacatcg cgagccccgc
     5221 cgagcagtgg gcggcgctcg gcgacggcta ccgcgtcagc gtgcgcatcg tcacgcgggc
     5281 gctggacgac gcgttgaagg tgccggtcag cgccgtgttc ccgcgcgccg acggcgccgg
     5341 catggcggtc ttcgtgatcg accgcggacg cgccaggctg gtgccggtcg agctgggcgc
     5401 gcgcaacggc gccgaaggct gggtgctgcg aggcctccag gccggcgcgc aggtgatcgt
     5461 ctacccgccc gtgggcgtgg gcgagggcgc gcgggtgcgc gcgcgccggg tctgacgcgc
     5521 gacggccccc gaccgacgac ccggacgccc gccgaacgac atggccgcca acgacacgct
     5581 cggcgtctgg atccccctgt cggccgcggt gggcatcggc ttgctggtcg ggctcgagcg
     5641 cgagcggcgc aaggcgctcg gcccgcggcg catccaggcc ggcatccgca cgttcacgct
     5701 ggtctcgctg ctcggggcgg cctgcgcctc ggcgcaggcg gactggatcc tgccggccat
     5761 ggtcgtggcc ctgtcggcgc tcatggcgac cgcctacgcc tgcagcacgg cgcccgacgc
     5821 gggcatcacg acgggcgccg cgctgctgct gacgctggcc ctcggcgcgc tggcgatgcg
     5881 cgagccggag gtggcggtgt cgatcggcgt ggtcgccacc atcgtgctgg cggcgcgtgc
     5941 gcgcctgcac gacttcgtgc gccggaccat cacggacgag gaactgcgcg acggcctcgt
     6001 cctcgcggcc gccgcgctgg tcatcctgcc gttcatcccg gatcgctacg tcgggccgat
     6061 gcaggccttc aacccgcgca ccacctggat catcgtcgtg ctcatcatgg gcgcgggggc
     6121 gatggggcac gtggcgcagc gcatgctcgg ggcgacctgg ggcctggccg cggccggctt
     6181 cgccgcgggc ttcgtctcca gcctcgcgac cgtgggcacg atgggagcgc gcgcgcgcca
     6241 ggtgccggcg gccgcgcgcg cggcggtgtc gggtgcgtt
//
LOCUS       DASVLN010000759         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_524212, whole genome shotgun sequence.
ACCESSION   DASVLN010000759 DASVLN010000000
VERSION     DASVLN010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524212"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /gene="gshA"
                     /locus_tag="VF453_18095"
     CDS             complement(<1..1343)
                     /gene="gshA"
                     /locus_tag="VF453_18095"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX7689622.1"
                     /translation="MRGIQERLKALSAARLQGMGRGVEKESLRVTAAGELAQTPHPAA
                     LGSALTHPHITTDFSEAQLELITGVHANAEACEAELTQVHQAVYRALGDEMMWVGSMP
                     CSLPPDDRIPLGQYGKSNVGRSKTVYRAGLGHRYGRRMQTISGIHYNWSLPEVTTAEY
                     FGLIRNFRRRSFVLLYLFGASPAVPASFLAGGPHVLEPLAPDTAHMPNATSLRMGRLG
                     YQSDAQTSLCVSYNSLDSYAASLADALVRPYPAYEKIGIRGPDGEYRQLSDTLLQIEN
                     EFYGTIRPKRVIQPGERPLHALRERGVEYIEVRCMDLDPFVPAGIAPATMRMIDVFLL
                     HCLLDDSPLDSPYEIAELARNQQRVAAHGREPGLKLERGGAEVLMSDWLTEILTECAP
                     IAARLDALEGTDAHARALAAARAAVMAPQTLPSARVLAAMKEGHGGSHDAFLRARS"
     gene            complement(1607..2398)
                     /locus_tag="VF453_18100"
     CDS             complement(1607..2398)
                     /locus_tag="VF453_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX7689623.1"
                     /translation="MSEDRKAALRVGKRIPGGKGVSGVLVKRGSELALDWAARQAARL
                     EATDSRGRALEIALPAGTALRAGDVLVAEDGSLMRVTAAPQPVFAVTPCPEHGSPVDL
                     LRAAYHLGARHVALALEADRLLVERDPALAELLRGEHLIVAEATAAFEPESGAWGTGG
                     HGHAHGHAHHGHHHGHGHGHGHGHGHDHDHDHAHGHGHEHGHEHAHGHAHDHGHAHHH
                     GHHPDEPKAAGKPVGVQVVAAPHVHGPGCGHDHDHDHDHGHGHGR"
     gene            complement(2519..>4542)
                     /locus_tag="VF453_18105"
     CDS             complement(2519..>4542)
                     /locus_tag="VF453_18105"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX7689624.1"
                     /translation="AAARAPDASARPDGPLDALPYHPVFDPADMDTTADPCVDFYRYA
                     CGGWQQANPIPADQSSWDVSSKMLLDNKRMLWGILDRLARQPDGRTPSQQKIGDAFAA
                     CMDEARIDELGGRPLLPQLERVAAIGSKSDLPRVLASLQRETNSAGLFFGFGAAPDYA
                     DSSQVIAFATAGGLGLPDRDYYLKTDAKSREQREAYRAHVQRMFGLLGDDEAASRREA
                     DVVLSIETALARGTMTNVAARDSRNLFHKVDLRGLRRAAPAFDWAPFLDALGLPRQKT
                     FNVTDPGFYRAFDRELRTRSLDDIKTYLRWHLVHAAAPSLSHEFVDEDFAFFHHVLEG
                     TPQLAPRWRRCVTHVDDQLGDALGQEFVKVAFSPELKARTVHMTRQIEDAMDGEIRNL
                     DWMSPLTKAKALEKLHSIANKIGYPERWRDYSALEIRRDDFFGNVTRADAFEFDRQLA
                     KIGKPVDRGEWTMTPPTVNAYYDPQMNDINFPAGILQPPLFDAKLDDAPNYGDTGATI
                     GHELTHGFDDDGRQYDAHGNLRNWWTKKDEAQFKQRTQCLVDQYKQYVIVDDLHINSR
                     LTLGEDLADFGGMVLALIAYHAEEAQSPHPDTDRDGFTPIQRFFIGEAQWACANERPE
                     ELRLRGLTDPHSPPKYRVNGLVANFKEFETAFHCKPGQPMAPVKRCRVW"
BASE COUNT          557 a         1525 c         1731 g          729 t
ORIGIN      
        1 gagcgggcgc gcaggaaggc gtcgtgcgag ccgccgtggc cctccttcat ggcggcgagg
       61 acgcgcgccg acggcagcgt ctgcggcgcc atcaccgcgg cccgcgccgc ggccagcgcg
      121 cgcgcgtggg cgtcggtgcc ctcgagggcg tccagccgcg ccgcgatcgg cgcgcattcg
      181 gtgaggatct ccgtcagcca gtccgacatc agcacctcgg cgccgccgcg ctcgagcttg
      241 agccccggct cgcgcccgtg cgccgcgacg cgctgctggt tgcgcgcgag ctcggcgatc
      301 tcgtagggcg agtcgagcgg gctgtcgtcg agcaggcagt gcagcaggaa gacgtcgatc
      361 atgcgcattg tcgcgggcgc gatgccggcc ggcacgaacg ggtcgaggtc catgcagcgc
      421 acctcgatgt actcgacgcc gcgctcgcgc agcgcgtgca gcggtcgctc gccgggctgg
      481 atcacgcgct tgggccggat cgtgccgtag aactcgttct cgatctgcag cagcgtgtcg
      541 ctcagctggc ggtactcgcc gtcggggccg cggatgccga tcttctcgta ggccgggtag
      601 ggccgcacga gcgcgtcggc gagcgacgcg gcgtagctgt cgaggctgtt gtagctcacg
      661 cacagcgagg tctgcgcgtc gctctggtag ccgagccggc ccatgcgcag cgaggttgcg
      721 ttgggcatgt gcgcggtgtc gggcgcgagc ggctccagta cgtgcgggcc gccggccagg
      781 aagctcgcgg gcaccgccgg cgacgccccg aacaggtaca gcagcacgaa cgagcggcgg
      841 cggaagttgc ggatcaggcc gaagtactcg gccgtcgtca cctcgggcag cgaccagttg
      901 tagtggatgc ccgagatcgt ctgcatgcgc cgcccgtagc ggtggccgag gccggcgcgg
      961 tagaccgtct tggagcgccc gacgttggac ttgccgtact gcccgagcgg gatccggtcg
     1021 tcgggcggca ggctgcaggg catgctgccg acccacatca tctcgtcgcc gagcgcgcgg
     1081 tagacggcct ggtggacctg cgtgagctcc gcctcgcagg cctcggcgtt ggcatgcacg
     1141 ccggtgatca gctccagctg cgcctcgctg aagtcggtgg tgatgtgcgg atgcgtcagc
     1201 gccgagccga gcgccgccgg atgcggcgtc tgcgcgagct cccccgccgc ggtcacgcgc
     1261 aggctctcct tctcgacgcc gcggcccatg ccttgcaggc gagccgcgct cagggccttc
     1321 aggcgttctt gtattccgcg catggcggtc tcctggaatc gtgatggccg cgaaggtacc
     1381 ggaatccggc gggccgtgca gcggacgggc tgatgacggg cgttcgcgac ttgtgctagg
     1441 ggcgatcacg cgccttgtac accctggccg tgacagtcgc ggggcgcgtg cgcggcccgt
     1501 cgccgccgag tacgggcaag ccgcggtccg gacgcgcttg gcggtgcggg tttcctcggg
     1561 gttcgccgaa gctcaggccg ggcgaggcgg cggcgccacg cggagttcag cggccgtggc
     1621 cgtgcccgtg gtcgtggtcg tgatcgtggt cgtgcccgca gcctggcccg tgcacgtgcg
     1681 gcgcagccac gacctgcacc ccgaccggct tgccggcggc tttcggctcg tccgggtggt
     1741 ggccgtggtg atgggcgtgg ccgtggtcgt gcgcgtgacc gtgcgcatgc tcgtgcccgt
     1801 gttcatgccc atggccgtgg gcatgatcgt ggtcgtggtc gtggccgtgg ccgtggccgt
     1861 ggccgtggcc gtggccgtga tggtggccgt ggtgcgcgtg gccgtgcgca tgaccatggc
     1921 cgcccgtgcc ccaggccccg gattccggct cgaaggcggc ggtggcctcg gcgacgatca
     1981 ggtgctcgcc gcgcagtagt tcggccagcg cgggatcgcg ctcgaccagc agccggtccg
     2041 cctccagcgc gagcgcgacg tggcgcgcgc cgaggtggta ggccgcgcgc agcaggtcca
     2101 ccggcgagcc gtgctccggg cagggcgtca ccgcgaacac cggctgcggc gcggccgtca
     2161 cgcgcatcag cgagccgtcc tcggccacca gcacgtcgcc ggcgcgcagc gcggtgcctg
     2221 cgggcagggc gatctccaac gcccggccgc gcgaatcggt tgcctccaga cgtgctgcct
     2281 gccgcgcagc ccagtcgagc gcgagctcgc tgccgcgctt cacgagcacc ccgctcacgc
     2341 ccttgccgcc ggggatgcgc ttgccgaccc gcagggcggc cttcctgtct tcactcatcg
     2401 ctggaactcc ttcgagggcg gcagcttacg cgcttcgccg gcgccgtgcc cgccgaagcg
     2461 gttccggccg cgcaggaggt ccgaccgcga cgactcgccg gcggcgcgcg gccgacgctc
     2521 accagacgcg gcagcgcttg acgggggcca tcggctggcc cggcttgcag tggaacgcgg
     2581 tctcgaactc cttgaagttc gcgaccaggc cgttgacgcg gtacttcggc ggcgagtgcg
     2641 gatccgtgag gccgcgcagc cgcagctcct cgggacgctc gttggcgcac gcccactgcg
     2701 cctcgccgat gaagaagcgc tggatcggcg tgaagccgtc gcggtcggtg tcgggatgcg
     2761 gcgattgcgc ctcctcggcg tggtacgcga tgagcgcgag gaccatgccg ccgaagtcgg
     2821 ccaggtcctc gccgagcgtc aggcggctgt tgatgtgcag gtcgtcgacg atcacgtact
     2881 gcttgtactg gtcgacgaga cactgcgtgc gctgcttgaa ctgcgcctcg tccttcttcg
     2941 tccaccagtt cctcaggttg ccgtgcgcgt cgtactggcg gccgtcgtcg tcgaagccgt
     3001 gggtgagctc gtggccgatc gtcgcgccgg tgtcgccgta gttgggcgcg tcgtcgagct
     3061 tcgcgtcgaa cagcggcggc tgcaggatgc cggcggggaa gttgatgtcg ttcatctgcg
     3121 ggtcgtagta ggcgttgacg gtcggcggcg tcatcgtcca ctcgccgcgg tcgaccggct
     3181 tgccgatctt cgcgagctgg cggtcgaact cgaacgcgtc ggcgcgtgtc acgttgccga
     3241 agaagtcgtc gcgccggatc tcgagtgccg agtagtcgcg ccagcgctcc gggtagccga
     3301 tcttgttggc gatggagtgc agcttctcga gcgccttcgc cttggtcagg gggctcatcc
     3361 agtcgaggtt gcggatctcg ccgtccatcg cgtcctcgat ctgccgggtc atgtgcaccg
     3421 tgcgcgcctt caattcgggg ctgaaggcga ccttcacgaa ctcctggccc agcgcgtcgc
     3481 cgagctggtc gtcgacgtgg gtgacgcagc gccgccagcg cggggcgagc tgcggcgtgc
     3541 cttcgagcac gtggtggaag aacgcgaagt cctcgtcgac gaactcgtgc gacagcgatg
     3601 gcgcggccgc gtggacgagg tgccatcgca ggtaggtctt gatgtcgtcg aggctgcgcg
     3661 tgcgcaactc gcggtcgaac gcgcggtaga agccggggtc ggtgacgttg aaggtcttct
     3721 gccgcggcag tccgagggcg tcgaggaagg gcgcccagtc gaaggccggc gccgcgcgtc
     3781 gcaggccgcg caggtcgacc ttgtggaaca ggttgcgcga gtcgcgcgcc gcgacgttcg
     3841 tcatcgtgcc ccgggcgagc gcggtctcga tggacagcac gacgtcggct tcgcggcggc
     3901 tcgcggcctc gtcgtcgccg agcagtccga acatgcgctg cacatgcgcg cgataggcct
     3961 cgcgctgctc gcgggacttg gcgtcggtct tcaggtagta gtcgcggtcg ggcagcccga
     4021 ggccgccggc ggtcgcgaag gcgatgacct gcgacgagtc ggcgtagtcc ggtgcggcgc
     4081 cgaagccgaa gaacaggccg gcgctgttgg tctcgcgctg cagcgacgcg agcacgcgcg
     4141 gcaggtcgct cttcgagccg atcgccgcga cgcgctcgag ctgcggcagc agcggccggc
     4201 cgccgagctc gtcgatgcgc gcctcgtcca tgcatgcggc gaaggcgtcg ccgatcttct
     4261 gctggctcgg cgtgcgcccg tcgggctggc gcgcgaggcg gtcgaggatg ccccacagca
     4321 tgcgcttgtt gtccagcagc atcttggagg agacgtccca gctcgactgg tccgcgggga
     4381 tgggattcgc ctgctgccag ccgccgcagg cgtagcggta gaagtcgacg cagggatcgg
     4441 ccgtcgtgtc catgtcggcc gggtcgaaga ccggatggta gggcagcgcg tcgagcgggc
     4501 cgtccggtcg tgcggaggcg tccggcgcgc gcgcggcggc ca
//
LOCUS       DASVLN010000760         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_308886, whole genome shotgun sequence.
ACCESSION   DASVLN010000760 DASVLN010000000
VERSION     DASVLN010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308886"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..554)
                     /locus_tag="VF453_18110"
     CDS             complement(57..554)
                     /locus_tag="VF453_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX7689625.1"
                     /translation="MKHVKKSVLLWYSAREMYELVVDVEQYPKFLPWCESAAVLERRA
                     ADPVAGTPQGMTARLGLSYAGVRQSFTTRNEEVPGESVRLALVDGPFSSLDGTWTFRP
                     LKPVDPAAPPTPDEPVACRIEFDLAYAFSNRVFEAVLSPVFDQVANTFVDSFVKRAAQ
                     VYGER"
     gene            584..1054
                     /gene="smpB"
                     /locus_tag="VF453_18115"
     CDS             584..1054
                     /gene="smpB"
                     /locus_tag="VF453_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX7689626.1"
                     /translation="MAALIAENRRARFEYHIEETYEAGVVLEGWEVKAIREGQVQLTD
                     GYVLIREGELHMIGCRINALRSASTHVHPEADRTKKLLMKRAEIRRLIGKVEQRGFTL
                     VPLNLHFKGSYVKADIALAKGKAEHDKRNTVKERDWEREKGRLMRHKVSGPSKG"
     gene            complement(1087..>1816)
                     /locus_tag="VF453_18120"
     CDS             complement(1087..>1816)
                     /locus_tag="VF453_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX7689627.1"
                     /translation="LEFPELPALVRRVRAGAPRATVALDNTWGAGLAFDAFALVPGAG
                     PEASVDLTIHALTKYPSGGGDVLMGSVVTRSQPLYERLALTHSRLGLGVGANDVEFVL
                     RALPSMPLRYAAQDAAGRRIAQWAQGRREVVRVLHPATPGSPGHEHWAALCRAAAGLV
                     TLEFDPAYAPERIDAFVDGLRLFGIGWSWGGPMSLAVPYRPEALRGAGSRYRGVLVRL
                     CVGLESVDDLVADLEAGLAALGAP"
BASE COUNT          300 a          652 c          657 g          207 t
ORIGIN      
        1 tgcgcagcgc ccgcggcgcg gggctccacg cgacctcgac ggcgatcgcg gcggcctcag
       61 cgctcgccat agacctgcgc ggcgcgcttc acgaaggaat cgacgaaggt gttggcgacc
      121 tggtcgaaga ccgggctcag caccgcctcg aagacgcggt tggagaacgc gtaggcgagg
      181 tcgaactcga tgcggcaggc gaccggctcg tccggggtcg gcggcgcagc cgggtcgacc
      241 ggcttgagcg gccggaacgt ccacgtgccg tccaggctcg agaacgggcc gtcgaccagc
      301 gcgagccgca ccgactcgcc cggcacctcc tcgttgcgcg tcgtgaagga ctggcgcacg
      361 cccgcgtagc tcaggcccag ccgggccgtc atgccctggg gcgtgcccgc gacggggtcg
      421 gcggcgcgcc gctcgaggac ggcggccgat tcgcaccagg gcaggaactt cgggtactgc
      481 tcgacatcga cgacgagctc gtacatctca cgggccgagt accagagcag gacggacttc
      541 ttgacgtgct tcatacaatg ggccggaatt ctaagcgtcc accatggcag ccctgatcgc
      601 agagaaccgc cgcgcgcggt tcgaatacca catcgaagag acctacgagg ccggcgtcgt
      661 gctggagggc tgggaggtca aggccatccg cgaaggccag gtccagctca ccgacggcta
      721 cgtgctgatc cgcgagggcg agctgcacat gatcggctgc cgcatcaacg cgctgcgctc
      781 ggcctcgacc cacgtgcacc ccgaggccga ccgcaccaag aagctgctga tgaagcgcgc
      841 ggagatccga cggttgatcg gcaaggtgga gcagcgcggc ttcacgctcg tgccgctgaa
      901 cctgcacttc aagggcagct acgtgaaggc ggacatcgcg ctcgccaagg gcaaggccga
      961 gcacgacaag cgcaacacgg tcaaggaacg cgactgggaa cgcgagaagg gccgcctgat
     1021 gcggcacaag gtgtcggggc cctcgaaggg ctgaagcgcc ctcgccgcgc ccgtcgccgt
     1081 cgcgcctcag ggtgcgccga gcgccgcgag cccggcctcg aggtcggcga ccaggtcgtc
     1141 gaccgactcc agcccgacgc acagccgcac gagcacgccg cggtagcgcg agcccgcgcc
     1201 gcgcaacgcc tccggccggt acggcaccgc gaggctcatc ggcccgcccc acgaccagcc
     1261 gatgccgaac agccgcagcc cgtcgacgaa cgcgtcgatg cgctcgggtg cgtaggcggg
     1321 atcgaactcc agcgtgacca gccccgcggc ggcgcggcac agcgcggccc agtgttcgtg
     1381 gccgggcgat ccgggcgttg ccggatgcag cacgcgcacg acttcgcgcc ggccctgcgc
     1441 ccactgcgcg atgcggcgtc ccgcggcgtc ctgcgcggcg tagcgcagcg gcatcgaggg
     1501 aagcgcgcgc agcacgaact cgacgtcgtt ggcgccgacg cccaggccca gccggctgtg
     1561 cgtgagcgcg aggcgctcgt acagcggctg cgagcgcgtc acgaccgagc ccatcagcac
     1621 gtcgccgccg cccgaggggt acttcgtcag cgcgtggatc gtgaggtcga ccgaggcctc
     1681 ggggccggcg cccggcacca gcgcgaacgc gtcgaaggcg agacccgcgc cccaggtgtt
     1741 gtcgagggcg accgtcgcgc gcggcgcgcc cgcgcgcacg cgccgcacga gcgccggcag
     1801 ctcggggaac tcgagc
//
LOCUS       DASVLN010000761         8261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_200581, whole genome shotgun sequence.
ACCESSION   DASVLN010000761 DASVLN010000000
VERSION     DASVLN010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200581"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1082)
                     /gene="iolG"
                     /locus_tag="VF453_18125"
     CDS             complement(87..1082)
                     /gene="iolG"
                     /locus_tag="VF453_18125"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEX7689628.1"
                     /translation="MKNVAVFGAGRIGRIHAANLAALPGVALKFVCDPVGDAAAKLAG
                     ELGAQVSTPEAVFADASIDVVAIAAPTDTHSDLIGRAAAAGKHIFCEKPIDLSVARAQ
                     ACADAVRAAGVACMMGFQRRFDPTFNEAKQRLDAGAIGHPEMLVVTSRDPGAPPAHYL
                     KGSGGIFRDMLIHDFDIFRWILCGDGDEAAWIAATGSCLVDPEIASVPDIDSSAVTIR
                     TRKGRLAQINTSRRAAYGYDQRFEVLGSTGMLQCGNHRPSEVVQSDAGGVHADKPEHF
                     FLQRYREAYRLELAHFFEVLQSGGRFRTTIEDGVAAQKLADAASESLARGQAIEL"
     gene            complement(1135..2082)
                     /locus_tag="VF453_18130"
     CDS             complement(1135..2082)
                     /locus_tag="VF453_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012213572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX7689629.1"
                     /translation="MTRSPSKKPAPARAAMPLPVPVPETTPDAANAASAHGAVDALLE
                     RISAEFNDLSRQLKAIARYVELHRGHLGLERIQDVAERSGVQPSAVVRFAKHFGFSGW
                     TELQKVFRDGIAQQIAPSHNYQARIRDVIGHAQGRLSSADIAGEFIGGAIAGLRELQR
                     AVQTSALDDAVELLAHAPSIWVAGARRSFAAASYLTYALQHADRPIHLVTGVGAMHEG
                     QLRGLREGDVMIAISYAPYAEETGLVARQAVERGAKLIALTDSRMSPLAADAAVALLV
                     SESSTFGFRALTNTMALAQSLFIALAYRLELEGLPGAPR"
     gene            complement(2079..3113)
                     /locus_tag="VF453_18135"
     CDS             complement(2079..3113)
                     /locus_tag="VF453_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7689630.1"
                     /translation="MTLRQDSPLKVGIAGLGRLGRRHAEQLAFRTRGAVLASACSPVA
                     AELAWARDELGVATLHEDFDAFVRDPAIDAVVLVTPTSLHADQTIAALEAGKHVFVEK
                     PLALDLGDCARVEAVAARHPGRVAMVGFVRRFDPSYARAQADVAGGAIGQPFLVRSQT
                     CDRNDPDGFFVRFAPTSGGIFMDCSVHDIDLARWMLGRPRATRAFASGTIALHEGLRP
                     IGDVDNGLAIVEFEGGQRAVFYASRTLAHGHDTQTEVIGTAGQLLVGQRGERDRLVTS
                     TATGVAYQAVGDFFERFGTAFEREMQAFVDACRGTRPLELELSDATEATRIGLAITRS
                     LRSGQAEQVE"
     gene            3298..4338
                     /locus_tag="VF453_18140"
     CDS             3298..4338
                     /locus_tag="VF453_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7689631.1"
                     /translation="MTRSNRRLALKTLALAAAATLALAAGAAQAAGHYVLISHAPDSD
                     SWWNTIKNAVKQAGEDFDVTVDYRNPPSGDLADMSRLIEQAAASNVDGVITTIADYDV
                     LKGSIKKVTDKKIPLITINSGTTEQSEKLGAIMHVGQPEYAAGHGAGERAKAAGIKSF
                     LCVNHYATNPASFERCRGFADAIGADFKKSTLDSGEDPAGVEAKVSAWLRQNPHTQAV
                     LALGPLSASPSLKAIDKMGLKGKIWFATFDLSDDIARGIKDGSVQFAIDQQPYLQGYI
                     PVAVMATMKQMHTTDLKKVSDAVKANPKVKARFEEYGLAPVYGPRHILSGPGFVTKDN
                     IGKVEKYAGQYR"
     gene            4400..5557
                     /locus_tag="VF453_18145"
     CDS             4400..5557
                     /locus_tag="VF453_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7689632.1"
                     /translation="MTPPEAAMTTDRPVAPPPIDERVRAISPWRRLLGRPESGALSGT
                     VLVFALFAIAAGGSGMFSAEGIVNWGTVAGYLGVIAVGAALLMVAGEFDLSIGSMIGF
                     AGMLLAVPCLYGGWPVWLSALFAIAVACALGALNGWLVVRTGLPSFIVTLAFYFVLRG
                     LSLALSVLLTGKTIVSSNGGAVLSDLVAADPVVHALFQGHVLTGLFDAMARAGVIAAR
                     DDGTALAIGVPKVIVWWLALTAIGAFVLARTRAGNWILAVGGDANAAKNVGIPVARVK
                     IALFMLTALCAALFAILQVADAGSAAADRGLQKEFEAIIAAVIGGCLLTGGYGSVIGA
                     SLGALIFGVVQIGIGYTSIDNNWYRVFLGGMLLVAVLFNNFVRRRVALGRR"
     gene            5578..6366
                     /locus_tag="VF453_18150"
     CDS             5578..6366
                     /locus_tag="VF453_18150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7689633.1"
                     /translation="MASDLILQLEDVSKYFGPVIALSGVTLRLHRGEVHCLLGDNGAG
                     KSTLIKMLAGVHAPDGGQYLVDGRPVRFRSPREALDAGIATVYQDLALVPLMSVARNF
                     FMGREPVRRLFGALPVMDHALAERTAAERLAEMGIRVRDPGQLVGNMSGGEKQCLAIA
                     RAIHFGARVLILDEPTAALGVKQSVNVLKLIARARQRGISVIFITHNVNHAYPIGDRF
                     TLLNRGKSMGTHAKSEVSKERVLDMMAGGAEMQSLMQDLEGVTI"
     gene            6363..>8261
                     /gene="iolC"
                     /locus_tag="VF453_18155"
     CDS             6363..>8261
                     /gene="iolC"
                     /locus_tag="VF453_18155"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEX7689634.1"
                     /translation="MSAFTLDDAGAAARRFDLACLGRLAVDLYAQQVGARLEDVSSFA
                     KYLGGSSANIAFGAARLGLRAAMISRVGDEQMGRFLLESLQREGCDTSMVQVDPERLT
                     ALAILGLKDRDTFPLLFYRENCADMALDPAGIREEFIADCRALVVTGTHLSQPGVRAA
                     SERALEFAGRHRTLRVLDIDYRPVLWGLTRRGDGQTRYVPDAAVTASLQEMLPRFELL
                     IGTEEEFVIAGGVPGDLLASLRRIREITRAAMVVKLGPLGCCLVDGPVPARLEDAPTC
                     AGERVEVMNVLGAGDAFAAGLLTGLLRGQGFAEAARLANACGALVVSRHACAPAMPTP
                     AELAHWFGTPSPDAASDARLAHLHRTTAARARRDELNVLAFDHRSQFYELAREAGAPE
                     SRLPALKALLVRAVERVERSRGLQGRVGVLVDEVYGAQALHAATGRGWWVGRPVELPG
                     SRPLRFEGTASLGSRLVHWPREQVVKCLVHFHPDDAVDLRLEQEARLQEVWAATRDTG
                     HELLLEVIPPRRPGHEDDVDDAVLRAVARLYTIGLKPEWWKLAPMRPQGWAALAALVA
                     ARDPQCRGAVILGLSQPLAELARGFADGTHPIVKGFMVGRSLWAEPARAWLRGPGDER
                     GDDALVDAV"
BASE COUNT         1128 a         2964 c         3017 g         1152 t
ORIGIN      
        1 cgagtgtgca ccggccgcga tgcccgggcc cgccggcggt gcatcaagcc cccggcgggc
       61 ggcgcggcga cgcccgccgg cggcgctcac aactcgatcg cctgcccacg cgcgagcgac
      121 tcgctcgccg cgtcggccag cttctgggcg gcgacgccgt cctcgatcgt cgtgcggaag
      181 cgcccgcccg actgcagcac ctcgaagaaa tgcgcgagct cgaggcgata cgcttcccgg
      241 tatcgctgca ggaagaagtg ctcgggcttg tccgcatgca cgccgcccgc gtcagactgc
      301 acgacctcgc tcggccggtg attgccgcac tggagcatgc ccgtcgagcc cagcacctcg
      361 aagcgctggt cgtagccgta ggcggcgcgc ctgctcgtgt tgatctgcgc gagccggccc
      421 ttgcgggtgc ggatcgtgac cgccgacgag tcgatgtccg gcaccgaggc gatctcgggg
      481 tcgaccaggc acgagccggt cgccgcgatc caggccgcct cgtcgccgtc gccgcacagg
      541 atccagcgga agatgtcgaa gtcgtggatc agcatgtcgc ggaagatgcc gcccgagccc
      601 ttgaggtagt gcgccggcgg cgcgccggga tcgcggctcg tgacgaccag catctcgggg
      661 tggccgatcg cgccggcgtc caggcgctgc ttggcctcgt tgaaggtcgg gtcgaagcgg
      721 cgctggaagc ccatcatgca ggccacgccg gccgcgcgca ccgcgtccgc gcaggcctgc
      781 gcgcgcgcga ccgacaggtc gatcggcttt tcgcagaaga tgtgcttgcc cgcggcggcg
      841 gcgcgcccga tcaggtcgct gtgggtgtcg gtcggcgcgg cgatcgcgac gacgtcgatg
      901 gacgcgtccg cgaacacggc ctcgggcgtc gagacctgcg cgccgagctc gccggcgagc
      961 ttcgcggcgg cgtcgccgac gggatcgcag acgaacttca gcgccacgcc gggcagcgcg
     1021 gcgaggttgg cggcgtggat gcggccgatg cggccggcgc cgaagacggc gacgttcttc
     1081 atggggaggc tccgtggcgt tcgtgggtgt ggggacggtg tcgggcaggc gggctcagcg
     1141 cggcgcgccg ggcagcccct cgagctcgag ccggtaggcc agcgcgatga acaggctctg
     1201 cgccagcgcc atcgtgttgg tcagcgcgcg aaagccgaag gtgctgctct ccgagacgag
     1261 cagcgcgacg gcggcgtctg cggccagcgg gctcatgcgg ctgtcggtca gcgcgatcag
     1321 cttcgcgccg cgctccaccg cctggcgcgc gacgaggccg gtctcctcgg cgtagggcgc
     1381 gtagctgatg gcgatcatca cgtcgccctc gcgcaggccg cgcagctggc cctcgtgcat
     1441 cgcgccgacg ccggtcacga gatggatcgg ccggtcggca tgctgcagcg cgtaggtgag
     1501 gtagctcgcg gccgcgaacg agcgccgcgc gcccgcgacc cagatcgacg gcgcatgcgc
     1561 gagcagctcc accgcgtcgt ccagcgccga ggtctgcacg gcacgctgca gctcgcgcag
     1621 gccggcgatc gcgccgccga tgaactcgcc ggcgatgtcc gcgctggaca gccggccctg
     1681 cgcatggccg atgacgtcgc ggatgcgcgc ctggtagttg tggctcggcg cgatctgctg
     1741 ggcgatgccg tcgcgaaaga ccttctgcag ctccgtccag cccgagaagc cgaaatgctt
     1801 ggcgaagcgc accaccgccg acggctgcac gccgctgcgc tcggcgacgt cctggatgcg
     1861 ctcgagcccg agatggccgc ggtgcagctc gacgtagcgc gcgatcgcct tgagctggcg
     1921 gctcaggtcg ttgaactcgg cgctgatgcg ctccagcagc gcgtcgacgg cgccgtgcgc
     1981 ggaggcggcg ttggcggcgt ccggcgtcgt ctcgggcacc ggtaccggca ggggcatggc
     2041 ggcgcgcgcg ggggcgggct tcttcgacgg cgagcgcgtc attccacctg ctccgcctgc
     2101 ccgctgcgca gcgagcgcgt gatcgccagg ccgattcgcg tggcctcggt cgcgtcggac
     2161 agctcgagct ccagcggccg cgtgccgcgg caggcgtcga cgaaggcctg catctcgcgc
     2221 tcgaaggcgg tgccgaagcg ctcgaagaag tcgccgaccg cctggtaggc gacgccagtg
     2281 gccgtgctcg tgacgaggcg gtcgcgttcg ccgcgctggc ccacgagcag ttggccggcc
     2341 gtgccgatca cctcggtctg cgtgtcgtgg ccgtgcgcga gggtgcgcga ggcgtagaag
     2401 acggcgcgct ggccgccctc gaactcgacg atcgcgaggc cgttgtcgac gtcgccgatg
     2461 gggcgcaggc cctcgtgcag cgcgatcgtg ccgctcgcga aggcccgcgt ggcgcgcggg
     2521 cggcccagca tccagcgcgc gaggtcgatg tcgtgcacgc tgcagtccat gaagatgccg
     2581 cccgaggtcg gcgcgaagcg cacgaagaag ccgtcggggt cgttgcggtc gcaggtctgc
     2641 gatcgcacga ggaagggctg gccgatcgcg ccgccggcga cgtcggcctg cgcgcgcgcg
     2701 tagctcgggt cgaagcggcg cacgaagccg accatcgcga cgcgcccggg atggcgcgcg
     2761 gccacggcct cgacgcgcgc gcagtcgccg aggtccagcg cgagcggctt ctcgacgaac
     2821 acgtgcttgc cggcctccag tgccgcgatc gtctggtcgg cgtgcagcga ggtcggcgtg
     2881 acgaggacga cggcgtcgat cgccgggtcg cgcacgaagg cgtcgaagtc ctcgtgcagg
     2941 gtcgcgacgc cgagctcgtc cctcgcccat gccagctcgg cggcgaccgg gctgcaggcg
     3001 ctcgcgagca ccgcgccacg ggtgcggaag gcgagctgct ccgcatggcg gcggccgagg
     3061 cggccgaggc cggcgatgcc gaccttcaat ggagagtcct ggcgaagggt catggcgccc
     3121 atgatagttc gcgccatcct gatttagaaa atgttttcta cgtacttgca ctcatagaac
     3181 agtatttgca tagcgcctag gatcgcctcc gacgcccccg gggatcggcg cgcgcaggtc
     3241 gcgcacgccg ccgggagcgg tccaccgacc acgagcacca acagaggaga catgtccatg
     3301 acccgttcga acagacgcct cgccctgaag accctggcgc tggccgccgc ggcgacgctc
     3361 gccctcgccg ccggcgccgc gcaggccgcc ggccactacg tcctcatcag ccacgccccc
     3421 gattccgact cgtggtggaa cacgatcaag aacgcggtca agcaggcggg cgaggacttc
     3481 gacgtcaccg tcgactaccg caatccgccc agcggcgacc tggccgacat gtcgcgcctg
     3541 atcgagcagg ccgcggccag caacgtcgac ggcgtgatca cgacgatcgc cgactacgac
     3601 gtgctcaagg gctcgatcaa gaaggtgacg gacaagaaga tcccgctgat cacgatcaac
     3661 tcgggcacga ccgagcagag cgagaagctc ggcgcgatca tgcacgtcgg ccagcccgag
     3721 tacgccgccg gccacggcgc cggcgagcgc gcgaaggccg cgggcatcaa gagcttcctg
     3781 tgcgtgaacc actacgcgac gaacccggcc tcgttcgagc gctgccgcgg cttcgccgac
     3841 gccatcggcg ccgacttcaa gaagtcgacg ctggactccg gcgaggatcc ggcgggcgtc
     3901 gaagccaagg tctcggcgtg gctgcgccag aacccgcaca cgcaggcagt gctggcgctc
     3961 gggccgctgt cggcgagccc gtcgctgaag gcgatcgaca agatgggcct gaagggcaag
     4021 atctggttcg ccaccttcga cctgagcgac gacatcgcgc gcggcatcaa ggacggctcc
     4081 gtgcagttcg cgatcgacca gcagccgtac ctgcaggggt acatcccggt cgcggtaatg
     4141 gcgacgatga agcagatgca caccaccgac ctgaagaagg tctcggacgc cgtgaaggcc
     4201 aatccgaagg tgaaggcacg cttcgaggaa tacggcctcg cgccggtcta cggcccgcgc
     4261 cacatcctgt ccggcccggg cttcgtgacc aaggacaaca tcggcaaggt cgagaagtac
     4321 gcgggccagt accgctgatc ccgcgcgccg ccgcggcccg cgcgccgcgg ccgcgaccca
     4381 gaccaccagg agacacgcca tgaccccgcc cgaggctgcc atgacgaccg accgtccggt
     4441 cgcgccacca ccgatcgacg aacgcgtgcg cgccatcagc ccgtggcgcc ggctgctcgg
     4501 ccgccccgag tcgggcgcgc tctcgggcac cgtgctcgtg ttcgcgctgt tcgcgatcgc
     4561 ggcgggcggc tccgggatgt tcagcgcgga gggcatcgtc aactggggca ccgtggcggg
     4621 ctacctcggc gtgatcgcgg tcggcgcggc gctgctcatg gtcgccggcg agttcgacct
     4681 gtccatcggc tcgatgatcg gcttcgccgg catgctgctc gcggtgccct gcctgtacgg
     4741 cggctggccg gtgtggctgt cggcgctgtt cgcgatcgcc gtggcgtgcg cgctgggtgc
     4801 gctcaacggc tggctggtcg tgcgcacggg gcttccgagc ttcatcgtca cgctcgcgtt
     4861 ctacttcgtg ctgcgcggcc tgagcctcgc gctgtcggtg ctgctgaccg gcaagacgat
     4921 cgtctcgagc aacggcggcg cggtgctgtc ggacctggtc gccgccgacc cggtcgtgca
     4981 cgccctcttc cagggccacg tgctcacggg cctgttcgac gcgatggcgc gcgccggcgt
     5041 catcgcggcg cgcgacgacg gcacggcgct cgcgatcggc gtgcccaagg tgatcgtctg
     5101 gtggctcgcg ctcaccgcga tcggcgcgtt cgtgctggcg cgcacgcgcg cgggcaactg
     5161 gatcctggcc gtcggcggcg acgccaacgc ggcgaagaac gtcggcatcc ccgtggcgcg
     5221 cgtgaagatc gccctgttca tgctgaccgc gctgtgcgcc gcgctgttcg cgatcctgca
     5281 ggtggccgac gccggctccg cggccgccga ccgcgggctg cagaaggagt tcgaggccat
     5341 catcgccgcg gtcatcggcg gctgcctgct gacgggcggc tacggctccg tgatcggcgc
     5401 gtcgctgggc gcgctgatct tcggcgtcgt gcagatcggc atcggctaca cgagcatcga
     5461 caacaactgg taccgcgtgt tcctcggcgg gatgctgctg gtggcggtgc tgttcaacaa
     5521 cttcgtgcgc cggcgcgtcg cgctggggcg gcgctgaaca ccggcgaagg gacaccgatg
     5581 gcatccgacc tgatcctgca gctcgaggac gtcagcaagt acttcgggcc ggtgatcgcg
     5641 ctgtccggcg tgacgctgcg cctgcaccgc ggcgaggtgc actgcctgct gggcgacaac
     5701 ggcgcgggca agagcacgct catcaagatg ctcgccggcg tgcacgcgcc cgacggcggg
     5761 cagtacctcg tcgacggccg gccggtgcgc ttccgctcgc cgcgcgaggc gctggacgcc
     5821 ggcatcgcga ccgtctacca ggacctcgcg ctcgtgccgc tcatgagcgt cgcgcgcaac
     5881 ttcttcatgg gccgcgagcc ggtgcgcagg ctgttcggcg cgctgccggt gatggaccac
     5941 gcgctcgccg agcgcaccgc cgccgagcgc ctggccgaga tgggcatccg cgtgcgcgac
     6001 ccggggcagc tggtgggcaa catgtccggc ggcgagaagc agtgcctcgc gatcgcgcgc
     6061 gccatccact tcggcgcgcg cgtgctgatc ctcgacgagc ccacggccgc cctcggcgtg
     6121 aagcagagcg tcaacgtgct caagctcatc gcgcgggcgc gccagcgcgg gatctcggtg
     6181 atcttcatca cccacaacgt caaccacgcg tacccgatcg gcgaccgctt cacgctgctc
     6241 aatcgcggca agtccatggg cacgcacgcg aagtccgagg tctccaagga gcgcgtgctc
     6301 gacatgatgg ccggcggcgc cgagatgcag tcgctgatgc aggacctgga aggcgtcacg
     6361 atatgagcgc attcaccctc gacgacgccg gcgcggccgc ccgccgcttc gacctcgcct
     6421 gcctcggccg gctcgccgtc gacctctacg cgcagcaggt cggcgcgcgg ctggaggacg
     6481 tctcgtcctt cgccaagtac ctgggcggca gctccgccaa catcgcgttc ggcgccgcgc
     6541 gcctcgggct gcgcgcggcg atgatctcgc gcgtcggcga cgagcagatg ggccgcttcc
     6601 tgctcgagtc gctgcagcgc gagggctgcg acacctcgat ggtgcaggtc gaccccgagc
     6661 gcctgaccgc gctcgcgatc ctcggcctga aggaccgcga caccttcccg ctgctgttct
     6721 accgcgagaa ctgcgccgac atggcgctcg atcccgcggg catccgcgag gagttcatcg
     6781 ccgactgccg ggcgctcgtg gtcacgggca cgcacctgtc gcagcccggc gtgcgcgcgg
     6841 cgtcggagcg ggcgctggag ttcgccggcc gccaccgcac gctgcgcgtc ctcgacatcg
     6901 actaccgccc cgtgctgtgg gggctgaccc gacgcggcga cggccagacg cgctacgtgc
     6961 ccgacgccgc cgtcaccgcg agcctgcagg agatgctgcc gcgcttcgag ctgctgatcg
     7021 gcaccgagga ggagttcgtg atcgccggcg gcgtgccggg cgacctgctc gcctcgctgc
     7081 gccgcatccg cgagatcacg cgggcggcga tggtcgtcaa gctcgggccg ctgggctgct
     7141 gcctcgtcga cgggcccgtg cccgcacggc tcgaggacgc accgacctgc gccggcgagc
     7201 gcgtcgaggt gatgaacgtg ctcggcgccg gagacgcctt cgccgcgggc ctgctcaccg
     7261 gcctgctgcg cgggcagggc ttcgccgagg ccgcgcggct cgccaacgcg tgcggggcgc
     7321 tggtcgtctc gcgccatgcc tgcgcacccg cgatgccgac gccggccgag ctcgcgcact
     7381 ggttcggcac gccctcgccg gacgccgcct ccgacgcccg gctcgcccac ctgcaccgca
     7441 ccaccgcggc gcgggcgcgc cgggacgagc tcaacgtgct cgccttcgac caccgcagcc
     7501 agttctacga gctcgcgcgc gaggccggcg cgccggagtc gcggctgccc gcgctcaagg
     7561 cgctgctggt gcgcgcggtg gagcgcgtcg agcgctcgcg cgggctgcag ggacgcgtgg
     7621 gcgtgctggt cgacgaggtc tacggcgccc aggcgctgca tgcggcgacc gggcgcggct
     7681 ggtgggtcgg ccggccggtg gaactgcccg gctcgcgccc gctgcgcttc gagggcacgg
     7741 cctcgctggg cagccggctc gtgcactggc cgcgcgagca ggtcgtcaag tgcctggtgc
     7801 acttccaccc ggacgacgcc gtggacctgc gcctggagca ggaggcgcgc ctgcaggaag
     7861 tctgggcggc cacgcgcgac accggccacg agctgctgct cgaggtgatc ccgccgcgcc
     7921 gcccgggaca cgaggacgac gtcgacgacg ccgtgctgcg cgccgtcgcc cgcctctaca
     7981 cgatcggcct gaagcccgag tggtggaagc tcgcgcccat gcgcccgcaa ggctgggccg
     8041 cgctcgccgc gctggtggcc gcgcgcgacc cgcagtgccg cggcgccgtc atcctcggcc
     8101 tgagccagcc gctggccgag ctggcgcgcg gcttcgcgga cggcacgcat cccatcgtca
     8161 agggcttcat ggtcggtcgc tcgctgtggg cggagcccgc gcgcgcctgg ctgcgcggcc
     8221 ccggcgacga gcgcggcgac gacgctctgg tcgatgccgt g
//
LOCUS       DASVLN010000762         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_308933, whole genome shotgun sequence.
ACCESSION   DASVLN010000762 DASVLN010000000
VERSION     DASVLN010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308933"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1728
                     /locus_tag="VF453_18160"
     CDS             <1..>1728
                     /locus_tag="VF453_18160"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7689635.1"
                     /translation="APVPAPAPQAMSRPMPLDDGIDDAAAYDDTELLGHSSGHQPAAD
                     DADTVIAVAAPVRRATSTAPSLRLLGVLAMLLLALWLARLGSAPLFDVDEGAFAEATR
                     ELVHGHDWGSTTLNGVDRFDKPILVYWLQALSLKTFGATEFAVRLPSALCAFLWCLAL
                     GQFAWRRFGPQVAIAAAGILATSLLPMLIGRAATADSLLNLLLALSTLDLWRHLENGR
                     DAPRRRAFAWIGLGLLAKGPIAILVPAAAAGLWLLTGRDRSRLQRLVGDPLAWGLMIL
                     IAAPWYAYALWRHGMAFVDGFLLHHNVERFTGSLEGHAGSILYYVKVLPVAMMPWTPL
                     LVPILMHVRQRWKEPLGRFSMLWAAFVIGFFSLSGTKLPHYVLYGFTPLVLLAAQELV
                     AVRRGVRVALFVTIAAMVALVSALPWIAHAAAAGTPDPLYRTLLSTAPPAPWWWLGIP
                     IGLLVFALTIWVPNVRNYLSEAEGALAAAGLAAAWLVFAALPWLGQTLQDPIRVLART
                     VPIAQLNLQDREPVVQWRLHQPSIAYYLGQTVERADPVGGDWALARADRLTPEEMARL
                     EVVQSVRGY"
BASE COUNT          187 a          646 c          625 g          270 t
ORIGIN      
        1 cggcgcccgt gcccgcgccc gcgccgcagg cgatgtcgcg cccgatgccg ctcgacgacg
       61 gcatcgacga cgcggccgcc tacgacgaca cggagctcct cgggcattcg tcgggccacc
      121 agccggccgc cgacgacgcc gacacggtga tcgccgtcgc ggcgccggtg cgccgggcga
      181 cgtccaccgc gccgtcgctg cgcctgctgg gcgtgctggc gatgctgctg ctcgcgctct
      241 ggctcgcgcg gctcgggtcg gcgccgctgt tcgacgtcga cgagggcgcg ttcgccgagg
      301 ccacgcgcga gctcgtgcac ggccacgact ggggcagcac gacgctcaac ggcgtcgacc
      361 gcttcgacaa gcccatcctc gtctactggc tgcaggcgct gagcctgaag accttcggcg
      421 ccaccgagtt cgcagtacgc ctgccttcgg cgctgtgcgc attcctctgg tgcctcgcgc
      481 tgggacagtt cgcgtggcgg cgcttcggcc cgcaggtggc gatcgcggcg gcgggcatcc
      541 tcgcgaccag cctgctgccg atgctgatcg gccgcgccgc gaccgccgac tcgctgctga
      601 acctgctgct ggcgctgtcg accctcgacc tgtggcgcca cctcgagaac ggccgcgacg
      661 cgccgcggcg tcgcgcgttc gcgtggatcg gcctgggcct gctggccaag gggccgatcg
      721 cgatcctcgt gcccgccgcc gccgcaggcc tgtggctgct gacgggacgc gaccgctcgc
      781 gcctgcagcg cctcgtcggc gacccgctgg cctgggggct gatgatcctg atcgccgcgc
      841 cctggtacgc ctacgcgctg tggcgccacg gcatggcctt cgtcgacggc ttcctgctgc
      901 accacaacgt cgagcgcttc acgggctcgc tcgagggtca cgcgggcagc atcctctact
      961 acgtcaaggt gctgccggtc gcgatgatgc cgtggacgcc gctgctggtg cccatcctga
     1021 tgcacgtgcg ccagcgctgg aaggagccgc tgggccgctt ctcgatgctg tgggcggcgt
     1081 tcgtgatcgg cttcttctcg ctgtccggca ccaagcttcc gcactacgtg ctctacggct
     1141 tcacgccgct ggtgctgctg gcggcgcagg agctggtggc tgtccggcgc ggcgtgcggg
     1201 tggcgctgtt cgtgacgatc gcggccatgg tcgcgctcgt ctcggccctg ccctggatcg
     1261 cccacgcggc cgcggccggc acccccgacc cgctgtaccg gacgctgctg agcacggcgc
     1321 cgcccgcgcc gtggtggtgg ctggggatcc cgatcgggct gctcgtgttc gcgctcacga
     1381 tctgggtgcc caacgtccgc aactacctga gcgaggccga gggggcgctc gccgcggccg
     1441 ggctcgcggc cgcgtggctg gtgttcgccg cgctgccctg gctgggacag acgctgcagg
     1501 acccgatccg ggtgctcgcg cgcacggtgc cgatcgcgca gctgaacctg caggatcgcg
     1561 agcccgtcgt gcaatggcgc ctgcaccagc cgagcatcgc ctactacctg ggccagaccg
     1621 tcgagcgcgc cgacccggtg ggcggcgact gggcgctggc gcgcgccgac cgcctcacgc
     1681 cggaggagat ggcccggctc gaggtcgtcc agagcgtgcg cggctacg
//
LOCUS       DASVLN010000763         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_149904, whole genome shotgun sequence.
ACCESSION   DASVLN010000763 DASVLN010000000
VERSION     DASVLN010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149904"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VF453_18165"
     CDS             <1..1273
                     /locus_tag="VF453_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689636.1"
                     /translation="LGALPAAARVLELRAGSDALQRAYLGRRPECDWRVLPIAVDGRV
                     DVDDPAAQGLFDLVVIADALPWLADPAGLLEALSRRVAVGGRLVLGCVNHASVDALAA
                     LVDGDVGVVPANAPLAGQPRLMGPAALFRLLMDAGWMPALVDHAPVADVAAPVEAGLQ
                     AVARAFGRAPGGSVDRVHRMARLVVEARPLFVGTPELDVPALFDVLVPTTDERQLRLN
                     VEASSGLEEVGARIVSCRGAASPAHAIELGRPHLAADWVLLCHQDIHFPAGFGRRLNA
                     VLAAIPPERRATTLIGFVGMGTGPRAQGRVPAGFVVDRTDRADHPATDNAVSIDELAV
                     VMARDTVHRIDPALGWHLWATDLCLAAICDHHVFPRIVRLPLFHNSRTGWQLPADFHA
                     SAERLARKWAGFGPIHTLCGVIDAVPETQTA"
     gene            1270..>2275
                     /locus_tag="VF453_18170"
     CDS             1270..>2275
                     /locus_tag="VF453_18170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7689637.1"
                     /translation="MTAITTLIPAFKPEYLGETLLSLRRQSVRDFRVVISDDSPGAVI
                     TDMIRDRRFGAATDGMDLTVVRGPGNGRLNHLRLVDLWAGSTPFMHLLMDDDVLFPGF
                     YRTHLQAHARAAFSVTVSARWLSQDDAAPAWSLPLPAAVEDSPLRVVPLDAAQLFPTV
                     VPGCENWLGELSNMVWTADAAASYPRPPVEGLNYYGLLDIGAVLEAVARQPVGFIRDH
                     LSVFRQHGRQTTHNVGGHGHKVCMLVWAAYALHAWAQQRIDARDAVRAISTTVRRCLD
                     LYGEGDPVMDEFYALVQRRGGSLADLHTGFRAFWQTLLAAHPGTAPAAAADGELQGAA
                     V"
BASE COUNT          258 a          887 c          813 g          317 t
ORIGIN      
        1 gctgggtgcg ctgcccgcgg ccgcgcgcgt gctggagctg cgcgccggct ccgacgcgct
       61 gcagcgcgcc tacctcggcc gtcgcccgga atgcgactgg cgcgtcctgc cgatcgccgt
      121 ggacggtcgc gtcgacgtcg acgatcccgc cgcgcagggc ctcttcgacc tcgtcgtgat
      181 cgccgacgcg ctgccgtggc tcgccgatcc cgccggcctg ctggaggcgc tttcccggcg
      241 cgtggcggtc ggcggccggc tcgtgctggg ctgcgtcaac cacgcgagcg tggacgcgct
      301 ggccgcgctc gtcgacggtg acgtcggcgt ggttcccgcc aacgcgccgc tggcgggaca
      361 gccgcggctg atggggcccg cggcgctctt ccggctgctg atggatgcgg gctggatgcc
      421 cgcgctggtc gaccacgcgc cggtggccga cgtcgccgcg ccggtggagg cggggctgca
      481 ggccgtcgcg cgcgccttcg gccgcgcccc gggcggcagc gtcgaccggg tgcatcgcat
      541 ggcacgcctg gtcgtcgagg cgcgcccgct gttcgtcggc acgcccgagc tggacgtgcc
      601 cgccctgttc gacgtgctcg tgcccaccac cgacgagcgc cagctgcgcc tgaacgtcga
      661 ggcctcgagc ggcctggagg aggtcggcgc acgcatcgtc tcgtgccgcg gcgcggcctc
      721 gcccgcgcat gcgatcgagc tgggccggcc gcacctggcc gccgactggg tgctgctgtg
      781 ccaccaggac atccacttcc cggccggctt cggccgccgt ctgaacgcgg tgctcgcggc
      841 gatcccgccc gagcggcgcg ccaccacgct gatcggcttc gtcggcatgg gcacggggcc
      901 gcgcgcgcag ggacgcgtgc ccgccggctt cgtcgtcgac cgcaccgacc gggccgacca
      961 ccccgccacg gacaacgccg tctcgatcga cgagctggcc gtcgtcatgg cgcgcgatac
     1021 cgtgcaccgt atcgacccgg cgctgggctg gcacctctgg gccaccgacc tgtgcctggc
     1081 cgcgatctgc gaccaccacg tcttcccgcg catcgtgcgc ctgccgctgt tccacaactc
     1141 gcgcaccggc tggcagcttc ccgccgactt ccatgcctcg gcggagcgcc tcgcccgcaa
     1201 gtgggccggc ttcggcccca tccacacgct gtgcggcgtc atcgacgccg tgccggagac
     1261 ccagaccgca tgaccgccat caccaccctg atcccggcct tcaagcccga gtacctcggc
     1321 gagaccctgc tgagcctgcg gcgccagagc gtgcgcgact tccgcgtcgt catctccgac
     1381 gacagtcccg gcgcggtcat caccgacatg atccgcgacc gccgcttcgg cgccgcgacg
     1441 gacgggatgg acctgacggt cgtccgcggc cccggcaacg gccggctgaa ccacctgcgg
     1501 ctggtcgacc tgtgggccgg ctcgacgccg ttcatgcacc tgctgatgga cgacgacgtc
     1561 ctgttccccg gcttctaccg cacgcacctg caggcgcatg cgcgggccgc gttctcggtc
     1621 accgtgagcg cgcgctggct gagccaggac gacgcggcgc cggcctggtc gctgccgctg
     1681 ccggcggcgg tcgaggacag cccgctgcgc gtcgtgccgc tggacgcggc ccagctgttc
     1741 ccgacggtcg tcccgggctg cgagaactgg ctcggcgagc tgtccaacat ggtctggacc
     1801 gcggacgcgg cggcgagcta tccccgcccg ccggtcgagg gcctgaacta ctacgggctg
     1861 ctggacatcg gcgcggtgct cgaggccgtg gcgcggcagc cggtcggctt catccgcgac
     1921 cacctgagcg tcttccggca gcacggccgg cagacgacgc acaacgtcgg cggccacggc
     1981 cacaaggtct gcatgctggt gtgggcggcc tacgcgctgc acgcctgggc gcagcagcgg
     2041 atcgacgcgc gcgacgccgt ccgcgcgatc agcaccaccg tccgccgctg cctcgacctc
     2101 tacggcgaag gcgacccggt gatggacgag ttctacgcgc tcgtgcagcg ccgcggcggc
     2161 agcctggccg acctgcacac cggcttccgc gccttctggc agacgctgct ggccgcgcac
     2221 ccgggcaccg cgcccgcggc ggcggcggac ggcgaactgc agggcgcggc cgtgg
//
LOCUS       DASVLN010000764         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_524695, whole genome shotgun sequence.
ACCESSION   DASVLN010000764 DASVLN010000000
VERSION     DASVLN010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524695"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VF453_18175"
     CDS             complement(<1..1114)
                     /locus_tag="VF453_18175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7689638.1"
                     /translation="MTHPLPRVIAHLDMDAFYASVELLRYPMLRGQAVVVGGGRRHTP
                     VHNEDGTWTFARLENYVGRGVVTTSSYEARKFGVFSAMGMMKAAALAPHAILLPTDFD
                     SYRHYSRLFKGAVRELAPVIEDRGIDEIYIDLTDVPGIREPIGHDPLGGVRAFAHELK
                     NNVRRATGLTCSIGVTPNKLLSKIASELDKPDGLTVLTYEDIPARIWPLPVRRINGIG
                     PKAGARLDALGIHTIGELAARDVGWLAQHFTPNTAAWLHAVSHGIDDRPIVTDGEPVS
                     MSRETTFDRDLHARRDRAELSAIFTELCEHVAADLSRKGYVGRTIGIKLRFDDFRTVT
                     RDQTIPLPTCDPAEIRRLAGLCLKRVDLTRRLRLLGV"
     gene            1285..1992
                     /locus_tag="VF453_18180"
     CDS             1285..1992
                     /locus_tag="VF453_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX7689639.1"
                     /translation="MSSTTPTHPHARQRTPFARIVRLVRVRPRLFIVALLGVAVGLLL
                     PDRVAAHGVTRWLIAWNAGAWLYILLAAAMMIRSSQDHMRHRAQLQDDGKYAILGLTV
                     VAAIASLAAIGGELSVVKDMHGWIRGAHVALAGITVLASWGFIQVMFTLHYAHDYYLA
                     KCHGHRPGLDFPGEDLPDYGDFFYFAAVIGTSGQTADVSFVTKPMRRIGSLHCILAYL
                     FNTTVLALLINIGASLF"
BASE COUNT          292 a          772 c          764 g          334 t
ORIGIN      
        1 gcacgcccag caggcgcagc cggcgcgtga ggtcgacccg cttcaggcac aggccggcca
       61 ggcgccggat ctccgccggg tcgcaggtcg gcagcgggat ggtctggtcg cgcgtcacgg
      121 tgcggaagtc gtcgaagcgc agcttgatgc cgatcgtgcg gccgacgtag cccttgcgcg
      181 agaggtcggc ggcgacgtgc tcgcacagct cggtgaagat ggcagagagc tccgcgcggt
      241 cgcgcctcgc gtgcaggtcg cggtcgaagg tggtctcgcg gctcatcgac accggctcgc
      301 cgtcggtgac gatcgggcgg tcgtcgatgc cgtgcgagac cgcgtgcagc cacgccgcgg
      361 tgttcggcgt gaagtgctgc gccagccagc cgacgtcgcg cgccgcgagc tccccgatcg
      421 tgtggatgcc cagcgcatcc aggcgcgcgc ccgccttcgg gccgatgccg ttgatgcggc
      481 gcaccggcag cggccagatg cgcgcgggaa tgtcctcgta ggtcagcacg gtcaggccgt
      541 cgggcttgtc cagctcggag gcgatcttcg aaagcagctt gttgggcgtg acgccgatcg
      601 agcaggtcag gccggtggcg cggcgcacgt tgttcttcag ttcgtgggcg aacgcgcgca
      661 cgccgccgag cgggtcgtgg ccgatcggct cgcggatgcc gggcacgtcg gtcaggtcga
      721 tgtagatctc gtcgatgccg cggtcctcga tcaccggcgc gagctcgcgc acggcgccct
      781 tgaacaggcg cgagtagtgg cggtagctgt cgaagtccgt gggcagcagg atcgcgtggg
      841 gcgccagcgc ggcggccttc atcatgccca tcgcggagaa cacgccgaac ttgcgcgcct
      901 cgtagctcga ggtcgtgacg acgccgcggc cgacgtagtt ctcgaggcgc gcgaaggtcc
      961 aggtgccgtc ctcgttgtgc accggggtgt ggcgccggcc gccgcccacc accaccgcct
     1021 ggccgcgcag catcggatag cgcagcagtt cgaccgacgc atagaaggcg tccatgtcga
     1081 ggtgcgcgat cacgcggggc agcgggtggg tcatgtggat cttctgtatg catccacagt
     1141 atcccacgcc gcgcgggcgc atcgtcgcac gaggcttgcc cttgcgggtc gggacggccg
     1201 tcagcgccgc ttcaggcacc cgcgttcggc gccgcggacg ccaccacaat ccggaggccg
     1261 cgaccgccgc gggaaggccc tcgcatgtcc agcaccacgc ccacgcaccc ccacgcgcgc
     1321 cagcgcaccc ccttcgcccg catcgtccgc ctcgtgcggg tgcggccgcg gctgttcatc
     1381 gtcgcgctgc tcggggtggc cgtcggactg ctgctgcccg accgcgtcgc ggcccacggc
     1441 gtcacgcgct ggctgatcgc gtggaacgcc ggcgcctggc tgtacatcct gctggccgcg
     1501 gcgatgatga tccgctcgtc gcaggaccac atgcgccacc gcgcccaact gcaggacgac
     1561 ggcaagtacg cgatcctcgg cctgacggtg gtggccgcga tcgcgagcct ggcggcgatc
     1621 ggcggcgagc tctcggtggt caaggacatg cacggctgga tccgcggcgc gcacgtcgcg
     1681 ctcgcgggca tcacggtgct cgcgtcgtgg ggcttcatcc aggtgatgtt cacgctgcat
     1741 tacgcgcacg actactacct cgccaagtgc cacggccacc ggcccggcct cgacttcccc
     1801 ggcgaggatc tgcccgacta cggcgacttc ttctacttcg cggccgtcat cggcacctcg
     1861 ggccagacgg cggacgtctc gttcgtgacc aagcccatgc ggcgcatcgg ctcgctgcac
     1921 tgcatcctgg cctacctgtt caacaccacc gtgctcgcgc tgctgatcaa catcggggcg
     1981 agcctgttct gaaggattgc gacgcatgac cgccgaccaa gtccaccccg cgagcttgtc
     2041 gtattcggcc gccgccgaca cctacgtgcg cggccggccc gactacccgg ccgggctcga
     2101 cgcgtggctg cgcgagtcgc tcggcctcgc gcccggcgcg cgcgtactcg acctcggcgc
     2161 cg
//
LOCUS       DASVLN010000765         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_93548, whole genome shotgun sequence.
ACCESSION   DASVLN010000765 DASVLN010000000
VERSION     DASVLN010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93548"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1452)
                     /locus_tag="VF453_18185"
     CDS             complement(241..1452)
                     /locus_tag="VF453_18185"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7689640.1"
                     /translation="MKLARRLDRIDPFYVMACAKVAQEIARSPECDPARGGEPMLYLN
                     IGEPDFTATAPVVAAAEAMLRAGRTQYTEAVGLPALRERISRWYARRWGVDVPARRIV
                     VTAGASAALQLVCHALFEEGDEVLMPDPSYPCNRHFVAAAGAKAVLVDTSPATRFQLD
                     AAAVRAHWTPATRGVLLASPSNPTGTSIAPDELARIVGVVREHGGACIVDEIYQGLSY
                     DPAFERSALALGEDVISINSFSKYFSMTGWRLGWVVLPEALVEPVERLAQNLYICPSA
                     VAQHAALACFDDESLAECERRRDAFRRRRDYVVPELERIGLPVPVVPDGAFYVWADCS
                     AHDVDSWDFVMRMMRQAHVALTPGKDFGPLHARAHLRLSFATALPVLQETIRRLEREL
                     GDTRHAPLGES"
     gene            complement(1449..1937)
                     /locus_tag="VF453_18190"
     CDS             complement(1449..1937)
                     /locus_tag="VF453_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689641.1"
                     /translation="MTVLRFPPRARVPAALALAALLVLPAAVRAGPSDADMRFLFGYR
                     IALACVQQDPAGEQSDVGRDLARAKDFPGWPAIEARPFPSCLRRHRFLDQALCDDMLA
                     TLTMKDEPKDATVARVNAALQRHEAVLPQADDVLWLEDAAQEAPAAFTCPERVPHHQD
                     RP"
     gene            2145..>2351
                     /locus_tag="VF453_18195"
     CDS             2145..>2351
                     /locus_tag="VF453_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689642.1"
                     /translation="MPIPPELQQVARFAADAFGAPRPRATAYRDAEGHERLHVLDVPD
                     RPRPGLVSWATVGLASHRLPGPART"
BASE COUNT          306 a          834 c          907 g          304 t
ORIGIN      
        1 acggcatccc cggccgccgc tgcggcggcg ccggccccgg ctccgccggc gcgcgcggca
       61 gccgactcgt cgaccacggt cgactcgaag ctggattcgg cggcgtcgac ggtgtcggcg
      121 cgccggaagg gattccatcg catggggcgg aagttaaccg atggtgagcc cgcaattctg
      181 gctgcaggac gggaagttac cctgctgcgg gctacaaatg ctcacaacgt ggtgattgct
      241 tcacgactcc ccgagcgggg catgtcgggt gtcgccgagt tcccgctcca gccgccggat
      301 cgtctcctgc agcaccggca acgcggtggc gaacgagagc ctcaggtgcg cgcgcgcgtg
      361 cagcgggccg aagtccttgc cgggcgtcag cgcgacgtgc gcctggcgca tcatgcgcat
      421 gacgaagtcc cagctgtcga cgtcgtgcgc gctgcagtcg gcccagacgt agaaggcgcc
      481 gtccggcacc accggcacgg gcaggccgat gcgttcgagt tccggcacga cgtagtccct
      541 gcggcggcgg aaggcgtcgc ggcgccgctc gcattcggcg aggctctcgt cgtcgaagca
      601 ggcgagggcg gcatgctggg cgaccgcgct cgggcagatg tagaggttct gggccaggcg
      661 ctccaccggc tcgaccagcg cctccggcag gacgacccag cccaggcgcc agccggtcat
      721 gctgaagtac ttcgagaagc tgttgatcga gatgacgtcc tcgcccagcg ccagcgcgct
      781 gcgctcgaac gcggggtcgt agctcaggcc ctggtagatc tcgtcgacga tgcaggcgcc
      841 gccgtgctcg cgcacgacgc cgacgatgcg cgcgagctcg tccggcgcga tcgaggtgcc
      901 ggtcggattc gagggcgagg cgagcagcac gccgcgcgtg gccggcgtcc agtgcgcgcg
      961 cacggcggcg gcgtcgagct ggaagcgcgt cgcgggcgac gtgtccacca gcaccgcctt
     1021 cgcgcccgcg gcggcgacga aatggcggtt gcacggatag ctcgggtcgg gcatcagcac
     1081 ctcgtcgccc tcctcgaaca gcgcatggca gaccagctgc agcgccgccg acgcccccgc
     1141 cgtcacgacg atgcgccgcg ccggcacgtc gacgccccag cggcgcgcgt accagcggct
     1201 gatgcgctcg cgcagcgcgg gcaggccgac cgcctcggtg tactgcgtcc ggccggcgcg
     1261 cagcatcgcc tcggcggccg cgacgaccgg cgcggtggcc gtgaagtcgg gctcgccgat
     1321 gttcaggtac agcatcggct cgccgccgcg cgccgggtcg cactcgggcg agcgcgcgat
     1381 ctcctgcgcg accttggcgc aggccatgac gtagaacggg tcgatgcggt cgaggcggcg
     1441 ggcgagcttc atgggcggtc ctggtggtgg ggcacgcgct cggggcacgt gaacgccgcg
     1501 ggcgcctcct gcgcggcgtc ctcgagccag agcacgtcgt cggcctgcgg cagcaccgcc
     1561 tcgtggcgct gcagcgcggc gttcacgcgg gcgaccgtgg cgtccttggg ctcgtccttc
     1621 atcgtcagcg tggcgagcat gtcgtcgcac agcgcctggt cgaggaagcg gtgtcggcgc
     1681 aggcagctcg ggaagggccg ggcctcgatc gcgggccagc cggggaagtc cttggcgcgc
     1741 gccaggtcgc gcccgacgtc cgactgctcg cccgccggat cctgctgcac gcaggcgagc
     1801 gcgatgcggt agccgaacag gaagcgcatg tcggcgtcgg acgggccggc gcggacggcg
     1861 gcgggcagga cgagcagcgc ggcgagcgcg agggcggcgg gcacgcgggc ccgtggcggg
     1921 aagcggagaa cggtcatgga gggcggcgcc ggcgcacggg cgccggagtc cggcaggggg
     1981 cctggcgcgg cggtgtcgac attgtagaga gccgcgccgc ttgcgtcggc ggtcgccgcg
     2041 gtgccggcgc atccttcgtg cggggatttc ggcccgtcgc cgcatgcggg cacgccggct
     2101 gcatgcgagc ccgcacgtct ttcgatccgc caccgcccat gccgatgccc atcccgccag
     2161 agctccagca ggtcgcgcgc ttcgccgcgg acgcgttcgg cgctcccagg ccgcgggcga
     2221 ccgcctaccg ggacgccgag ggccatgagc gcctgcacgt gctggacgtc ccggaccggc
     2281 cgcgccccgg actcgtgtcg tgggcgacgg tgggcctcgc gtcgcaccgc ctgcccgggc
     2341 cggcgcgcac g
//
LOCUS       DASVLN010000766         6154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_93626, whole genome shotgun sequence.
ACCESSION   DASVLN010000766 DASVLN010000000
VERSION     DASVLN010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93626"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="VF453_18200"
     CDS             <1..1879
                     /locus_tag="VF453_18200"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEX7689643.1"
                     /translation="VRAALRRQQATQLTVVARGRKDALVGFGDDSTPGPSVELRSDVL
                     TGPHLEMQVGGRFDPVPDPRRQGHVLRFDDTALVAGGDSVQLEAWAHRPWWGPGWQTA
                     LAMGNNTPALDGVGIQRSSASTSTLPWLRWLGPWSAEGFVARADGMSDPPHPFIVGGR
                     IELRPFPWLQLGLERTAQWGGQGRDQTFLSFVNMLLSRGVNADTRAEIPHDPANEMAG
                     YDARIRCPLGLRCAVYGQAIGEDEAGHLPSRFLALGGAEFWSADGSQRVFLEAARTSA
                     YRDWFGPPLTDYAYRNYAYPQGYTDDGRWLGAGAGPDSRLFTLGWIDADADASVRVTA
                     GHVGSRIGTFSPVTFDPASSGKLLSVSARRTFAWGPSTWTPQVDWTRVDAAAGAWRQT
                     RVGLEMSLDLDAATNGATNGVFGRLATARPGWQTQALVAAALVGGSAIFDRRADDYAQ
                     DHRHDTTSLVLRPVGNAAPAIELGLAGTAWLASKDPRAAATGRTSVESGVVAVALAEA
                     IKLAVKRERPNRGYGPGSFGHASAKDSSFPSTHSTAAWALLTPVAEEYDAPWLYGVAA
                     ITNAGRVLAREHWLSDTVAGAALGWAVGHALHARQAGTGPDSPEFALGPGTVAVSVPF
                     R"
     gene            1926..2756
                     /locus_tag="VF453_18205"
     CDS             1926..2756
                     /locus_tag="VF453_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007743432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEX7689644.1"
                     /translation="MRAMEETMTMRADDEAALAEAVRLLERPSLGARLAGYVGSPIEK
                     AVGLLPRRASVLVGSAARKAIEAALKLALKTLAASDPRGAAPPPASTGWHKAASAVSG
                     AAGGAFGLAALGVELPVSTTIMMRSIADVARSEGADLHDRQVQLECVMVLGLGGTSPA
                     DDAAEVGYFAVREAMTRAVSSAAAHIAQHGLAREGAPALVRLIALVAERFSLNVTEKA
                     AAQLVPLVGAVGGAAVNAIFIDHFQSVARGHFTVRRLERRYGEAEVRVRYQALAATLA
                     "
     gene            3425..5365
                     /locus_tag="VF453_18210"
     CDS             3425..5365
                     /locus_tag="VF453_18210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX7689645.1"
                     /translation="MYKMTRGGLRAPAGLSVVSMAAALALGAMSMPAQAGLVGGSTPT
                     LASKARVGSDPRIVDQGVAASTDVKSITISLAVRNKAALDAAVAAVADPKNANFRKFI
                     TPAQFAATYGQTPAAVAQVVSYLQSKGFTITQVHANNLLISARATNAQIAAAFGSPVH
                     TYALGASTWQAPAAKPVMPAAISAIATGVTGLSTRSSFHSHAAAIPHAGALAGDAVQP
                     TVVPNGAIATDTPGSYTVGDVAAKYNVNPLYAKGLTGAGKTIGIATLAGYNQSDAFAY
                     WDAVGLTVDPNRITDVAVDGGPLAKDGPGSEGAGETTLDVEQSGGLAPGASMRVYLAP
                     NTDSGFIDVFAQAIDENQVDVLSVSWGAPEIFNDDATLATYDAIFEQAALQGIPVIAA
                     AGDAGAYDINRDYTYPDCTTLLDVDFPASDPNVLAAGGTTLPNTFQHKYGPVTVATER
                     AWGWDYMKNYIVTYYGNALYYADYFAVGGGGGVSVDFLKPSYQAGIAGTATSASAQSL
                     LCSPAFLGAPGTQYIDLVDMPAGVAGRNLPDVSLNADPYSGYLVYQGGWGSGSGGTSF
                     VAPQLNGILTLISSGLPGRVGPINPQLYAAFKAKGYAAGSPFKAITAGDNEFYKSTAS
                     YNPSTGLGSLDVAALATALGVK"
     gene            5448..6020
                     /locus_tag="VF453_18215"
     CDS             5448..6020
                     /locus_tag="VF453_18215"
                     /inference="COORDINATES: protein motif:HMM:NF038126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxDxF family PEP-CTERM protein"
                     /protein_id="HEX7689646.1"
                     /translation="MTIFKQLALAAALALVGVTGAQAATEIGGEYVPMDPGAPGDFTN
                     TWTLSNADFLAPAAAGGTEKGTKGDSFIDWFQFNVPESEYVTFGFSSQLNPTAEVSFT
                     GWVIGVPADGSIIDSQGSLITNALQGPTYFLASGTYEIDVFGTFKQRGGLFDGYIMGN
                     VPEPSSWALMLAGVGAIGALARRRKARAQA"
BASE COUNT          874 a         2264 c         2162 g          854 t
ORIGIN      
        1 cgtgcgcgcc gcgctgcgcc ggcagcaggc gacgcagctg acggtcgtcg cgcgcgggcg
       61 caaggacgcg ctcgtcggct tcggcgacga ctcgaccccc ggcccgagcg tcgagctgcg
      121 cagcgacgtg ctgacggggc cgcacctgga gatgcaggtc ggcggacgat tcgaccccgt
      181 gcccgacccg cgccggcaag gccacgtgct gcgcttcgac gacaccgcgc tggtggccgg
      241 aggcgactcg gtgcagctcg aggcgtgggc gcaccggccg tggtggggcc ccggctggca
      301 gaccgcgctg gccatgggca acaacacccc ggccctggac ggcgtgggca tccagcgctc
      361 gagcgcgagc acgtcgacgc tgccctggct gcgctggctc gggccgtgga gcgccgaggg
      421 cttcgtcgcc cgcgccgacg ggatgagcga tccgccgcat ccgttcatcg tcggcggccg
      481 catcgagctc cggccattcc cgtggctgca gctggggctc gagcgcaccg cgcaatgggg
      541 cggccagggt cgcgaccaga ccttcctgag cttcgtgaac atgctgctca gccggggtgt
      601 caatgcggac acgcgggcgg agatcccgca cgatcccgcc aacgagatgg ccggctacga
      661 cgcccgcatc cgctgcccgc tcgggctgcg ctgcgcggtg tacggccagg ccatcggcga
      721 ggacgaggcg ggccacctgc cgtcgcgctt cctggcgctc ggcggcgcgg agttctggag
      781 cgccgacggc agccagcgcg tcttcctgga ggccgcgcgc accagcgcct accgcgactg
      841 gttcggtccg cccctcaccg actacgccta tcgcaattac gcgtacccgc aagggtatac
      901 ggacgacggc cgctggctcg gtgccggcgc aggccccgat tcgcgcctgt tcacgctggg
      961 ctggatcgat gccgacgcgg acgccagcgt gcgcgtgacg gcaggccacg tgggctcgcg
     1021 catcggcacg ttcagccccg tcaccttcga cccggcgtct tcgggcaagc tgctgtcggt
     1081 gtccgcgcgc cgcaccttcg cgtgggggcc gtcgacgtgg acgccgcagg tcgactggac
     1141 gcgggtggac gccgcggccg gcgcctggcg ccagacgcgc gtcgggctgg agatgagcct
     1201 cgacctcgac gccgccacga acggcgcgac caacggcgtc ttcggccgcc tggcgacggc
     1261 gcgtccgggc tggcagaccc aggcgctcgt ggccgcggcg ctcgtcgggg gctcggccat
     1321 cttcgaccgc cgcgccgacg actacgccca ggatcaccgc cacgacacca cctcgctcgt
     1381 cctgcgcccc gtgggcaacg cggcgccggc catcgagctc gggctggccg gcaccgcgtg
     1441 gctcgcgagc aaggatccgc gtgccgccgc caccgggcgg acgtcggtgg agtccggcgt
     1501 cgtggccgtc gcgctggccg aggcgatcaa gctcgcggtc aagcgcgagc gcccgaaccg
     1561 cggctacggc ccgggcagct tcggccacgc gtcggccaag gactcgtcct ttccgtcgac
     1621 gcactcgacc gcggcctggg cgctgctgac gcccgtggcc gaggagtacg acgcgccgtg
     1681 gctctacggc gtcgccgcga tcaccaatgc gggccgggtg ctcgcgcggg agcactggct
     1741 gtccgacacc gtcgccggcg ccgcgctggg ctgggccgtc ggccacgccc tgcacgcgcg
     1801 gcaggccgga acggggccgg actcgcccga gttcgcgctc ggacccggca cggtcgcggt
     1861 gagcgtgccg ttccgctgat gacccgcggc gcgcaccggc ccgcggattg ccttcccgcg
     1921 ccatcatgcg cgcgatggag gagacgatga cgatgcgggc ggacgacgag gcggccctgg
     1981 ccgaggcggt gcggctgctg gagcgtccca gcttgggcgc gcggctggcg ggctacgtcg
     2041 gctcgccgat cgagaaggcg gtcggcctgc tgccgcggcg cgcctccgtg ctggtgggct
     2101 ccgcggcgcg caaggcgatc gaggcggcgt tgaagctcgc gctgaagacg ctggccgcga
     2161 gcgatccgcg cggcgcagcc ccgccgccgg cgtccaccgg ctggcacaag gcggcgagcg
     2221 cggtgagcgg cgcggccggc ggtgcgttcg ggctcgcggc gctgggcgtg gagctgcccg
     2281 tgtcgacgac gatcatgatg cgctccatcg ccgacgtggc gcgctccgag ggcgccgacc
     2341 tgcacgaccg ccaggtgcag ctggagtgcg tgatggtgct gggactcggc ggcacctcgc
     2401 cggcggacga cgcggccgag gtcggctact tcgcggtgcg cgaggcgatg acgcgcgccg
     2461 tctcgagcgc cgcggcccac atcgcgcagc acggcctcgc gcgcgagggc gcgcccgcgc
     2521 tcgtgcggct gatcgcgctg gtggcggagc gcttctccct caacgtcacc gagaaggcag
     2581 ccgcgcagct ggtaccgctg gtcggcgcgg tcggcggcgc ggcggtcaac gcgatcttca
     2641 tcgaccactt ccagtccgtc gcgcgcggcc atttcacggt gcggcggctg gagcggcgct
     2701 atggcgaggc cgaggttcgc gtgcgctacc aggcactcgc ggcgacgctc gcgtgaatgg
     2761 ggcggtacct gccgaccaag accctgacgc gcgcatgtga gggaaatccc tcgagtggtc
     2821 gagaactcac cccaagaacg tgggggagcc gggtcgcgtt aaagcggcca ggacgacgct
     2881 ccggaatgca ccatgcacca tgtcgacgcg ggttcatgtg gctgcggatc cgacatgcct
     2941 gcgtcatcct ggacatcccg ccgggcgggc gccgcgccaa ctcgatcccg ggtacgtgtc
     3001 gcgccagtcg ggaaggcgag catccacacc gcgtcgagcc cgcatggttg agcgacgggg
     3061 cgcgtccggg ccttggcgtg gcggcggcgc ccgcgggcgc tcgatcggct ggttatgcgg
     3121 ccaagacatg gcggctgccg acatggcctg cttcttgcga ccctgcgagg ggttcgccga
     3181 tacgcaagcg aaagccgaac gcaagcccgg ccctctgtca agagtgccta cgctatcggc
     3241 gtttcccgca tctctcgttt acggcaggcg gacggttttc tttagcgctt ggcttgtaaa
     3301 gttgccgggc agtcgatggg cccacgcccg tcagaccacg cccacgtccc gaccgcgtcg
     3361 aaggcgtacc acgtctgaac gctggatcga ttccctcgtc ttttcaaaca gaagagtgtt
     3421 gaacatgtac aagatgactc gcggaggact tcgcgccccg gctggcctgt ccgtcgtctc
     3481 gatggccgcg gccctggcgc tcggagcgat gtcgatgccg gcccaggccg gattggtcgg
     3541 cggcagcacg cccaccctgg ccagcaaggc gcgcgtgggc agcgacccgc gcatcgtcga
     3601 ccagggcgtc gccgcctcga cggacgtcaa gagcatcacg atctcgctgg ccgtgcgcaa
     3661 caaggccgcg ctcgacgccg ccgtcgccgc ggtcgccgac ccgaagaacg cgaacttccg
     3721 caagttcatc acgccggcgc agttcgccgc gacctacggc cagacgccgg ccgccgtggc
     3781 ccaggtcgtc tcgtacctgc agtccaaggg cttcacgatc acccaggtgc atgcgaacaa
     3841 cctgctgatc tcggcgcgcg cgaccaacgc gcagatcgcc gcggccttcg gcagccccgt
     3901 ccacacctac gcgctgggcg cgtccacctg gcaggcgccg gcggccaagc cggtgatgcc
     3961 ggccgcgatc tcggccatcg ccaccggcgt gaccggcctg agcacccgct cgtcgttcca
     4021 ttcgcacgcc gccgcgatcc cgcatgccgg cgctctggcc ggcgacgccg tccagcccac
     4081 cgtcgtgccg aacggcgcga tcgccaccga taccccgggt tcgtacaccg tcggcgacgt
     4141 cgccgccaag tacaacgtca acccgctgta tgccaagggc ctgaccggcg ccggcaagac
     4201 catcggcatc gccacgctgg ccggctacaa ccagtcggac gccttcgcct actgggacgc
     4261 cgtcggcctg acggtggacc cgaaccgcat caccgacgtg gcggtggacg gcggcccgct
     4321 ggccaaggac ggccccggct ccgaaggcgc cggcgagacg acgctggacg tcgagcagtc
     4381 cggcggcctc gccccgggcg ccagcatgcg cgtctacctc gcgccgaaca cggattccgg
     4441 cttcatcgac gtcttcgcgc aggcgatcga cgagaaccag gtcgacgtgc tgtcggtgag
     4501 ctggggcgcg cccgagatct tcaacgacga cgcgacgctg gccacctatg acgcgatctt
     4561 cgagcaggcc gcgctgcagg gcatcccggt gatcgccgcc gccggcgacg ccggcgcgta
     4621 cgacatcaac cgcgactaca cctatccgga ctgcacgacg ctgctggatg tcgacttccc
     4681 ggcttccgac ccgaacgtgc tggccgccgg cggcacgacg ctgcccaaca cgttccagca
     4741 caagtacggc cccgtgacgg tcgcgaccga gcgcgcctgg ggctgggact acatgaagaa
     4801 ctacatcgtc acgtactacg gcaatgcgct gtactacgcc gactacttcg cggtgggcgg
     4861 cggcggtggc gtcagcgtgg acttcctgaa gccctcgtac caggccggca tcgccggcac
     4921 ggcgacctcg gcctcggcgc aatcgctgct gtgcagcccg gcgttcctcg gcgcgcccgg
     4981 cacgcagtac atcgacctcg tcgacatgcc cgcgggcgtc gccggccgca acctgccgga
     5041 cgtctcgctc aacgccgacc cctacagcgg ctacctggtc taccagggcg gctggggctc
     5101 gggctcgggc ggcacgagct tcgtcgcgcc gcagctcaac ggcatcctga cgctgatctc
     5161 gtcgggcctg cccggccgcg tcggcccgat caacccgcag ctgtacgccg cgttcaaggc
     5221 caagggctac gccgcgggct cgccgttcaa ggccatcacg gccggcgaca acgagttcta
     5281 caagtccacc gcctcctaca accccagcac gggcctgggc tcgctggacg tggccgcgct
     5341 ggcgacggcg ctcggcgtga agtgatacgc gatgcgggct gctcgacgat aatcgccggc
     5401 gcagcctgct catctgcccc gaaccctaca agcgacgcaa caacatcatg accatcttca
     5461 agcaactggc cctcgcggcc gccctggccc tcgtcggcgt caccggcgcc caggccgcca
     5521 ccgagatcgg cggcgaatac gtgccgatgg atccgggcgc gcccggcgac ttcaccaaca
     5581 cctggacgct gagcaacgcc gacttcctgg cgccggcggc cgcgggcggc acggagaagg
     5641 ggaccaaggg cgactcgttc atcgactggt tccagttcaa cgtcccggaa agcgaatacg
     5701 tcaccttcgg cttcagcagc cagctcaacc ccaccgccga ggtctcgttc accggctggg
     5761 tcatcggcgt tccggccgac ggcagcatca tcgacagcca gggctcgctg atcacgaacg
     5821 cgctccaggg cccgacctac ttcctggcca gcggcacgta cgagatcgac gtgttcggca
     5881 cgttcaagca gcgcggcggc ctgttcgacg gctacatcat gggcaacgtg cccgagccct
     5941 cgagctgggc gctgatgctg gctggcgtcg gcgcgatcgg cgcgctggcg cgccgccgca
     6001 aggcccgggc gcaagcctga tgccggcggg ccgcacggcc cgcctgtctg acgaccggcg
     6061 cgagttccgc cagccggtcg atcgtcgagg aaggccgcga tccgtcgcgg ccttttttcg
     6121 ttccggcgat cgccgcgttc agcgcgtcgc ggcg
//
LOCUS       DASVLN010000767         6116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_150261, whole genome shotgun sequence.
ACCESSION   DASVLN010000767 DASVLN010000000
VERSION     DASVLN010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150261"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1777)
                     /locus_tag="VF453_18220"
     CDS             complement(89..1777)
                     /locus_tag="VF453_18220"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX7689647.1"
                     /translation="MTPQELLAQYGPRESMEYDVVIVGAGPAGLATAIRLKQLAAEAG
                     KEVSVVVLEKGSEPGAHILSGAVMDPKAITELLPNWQADGAPLNQPVTGDEFLFLSET
                     GSKSVPEWLMPYPFHNHGNYVISLSDVVKWLAGQAEALGVEIFPGFAAAEVLYDDQGA
                     VKGVATGNVGVGRDGEPHDGFQLGMELHGKYTIFAEGARGHLGKQLLAKYRLQEGRDA
                     QSWAIGIKELWEIPADKAKPGLVVHTAGWPITDATTFGGGFLYHLADNKVTLGFVIGL
                     DYQNPWMSPFEEMQRWKTHPAVRAHLEGGKRLSYGARAINNGGLQSLPKMVFPGGALV
                     GCDAGTLDAARIKGSHAAIKSGMMAAEAAFEALQAGRQADELSAYPAAFERSWLHKEL
                     QRTKNFKLWFKKGKLVGNVMTGLEQWLMPKLGMPIPPWTLHNTKKDNEALRPASEMPQ
                     IAYPKPDGKLTFDRLSSVFVSNTNHAEDQPVHLTLKDGGVPVKVNLAEYAGPESRYCP
                     AGVYEFVKNEDGSDRLQINAQNCVHCKTCDIKDPTQNIVWVTPEGGGGPNYSGM"
     gene            1934..2716
                     /locus_tag="VF453_18225"
     CDS             1934..2716
                     /locus_tag="VF453_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7689648.1"
                     /translation="MGMKIDLAGRVALVTGASSGLGAQFARTLAECGAGVVLAARRLE
                     RLKTLRAEIEANGGDAHVVELDVTDPDSIKAAVAHAETEMGVIDILVNNSGVSTTQKL
                     TDVTPEDFDYVFDVNTRGAFFVAQEVAKRMIARARGAAPGTYTGGRIVNVASMAGLRP
                     LSQIGVYAMSKAAVVHMTRAMALEWGRYDINVNALCPGYIDTEINHHHWSTEAGQKLV
                     QMTPRKRIGKPTDLDSALLMLCANESHFINGAVLQADDGFGI"
     gene            2735..3157
                     /locus_tag="VF453_18230"
     CDS             2735..3157
                     /locus_tag="VF453_18230"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7689649.1"
                     /translation="MRLELPDGLRLVHEMRIPVRWGDLDAMGHVNNTIYFRYFESLRI
                     DWLRAFGAEPNPAGVGPVMANGFCNFLRQIEYPGEIVARHFVGAVGTKSVDTYFTLSM
                     ADDPDTVCANGGATLVWLDFPRKTTVPLPDDLRAACTA"
     gene            complement(3170..4432)
                     /locus_tag="VF453_18235"
     CDS             complement(3170..4432)
                     /locus_tag="VF453_18235"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7689650.1"
                     /translation="MTLRRKPRWTFLGLCLGLCVATPAPSYAEPAPLRIGLVAPLTGG
                     SEDLGASTKFGAELAVQEINEAGGYLGRPLKLVERNDKSVPQVGEQIAHELVDKEKVD
                     FTIGYCNTGVALQAAKVFQASRSLLMIPCSQGTAVTRQFAAADSYIFRNAPYDLLNAK
                     FLVSEIVERRRLKKVAIFADKTGYGEGGLKDVTAELAARGLQPAYVARFAPGVESLAS
                     EMEQAAQSGAQAIVVYAVGPDQVTVLKGRRAAHVKLPLLSTWALSARSVLELGGPDLL
                     EGTMMVQTVIYDIYNERRAGFLAKYFKMSNDRPISSLMAAAQSYDAVYLMLAALFQTH
                     GDVSGPALKAALETLPRPIEGVVTTYDHPFDATDHDAISQNMLWLGVWHGGEIRFYYP
                     EDAKLSSVVRRKDHVGAPGEHAADAAQR"
     gene            complement(4633..5949)
                     /gene="xylA"
                     /locus_tag="VF453_18240"
     CDS             complement(4633..5949)
                     /gene="xylA"
                     /locus_tag="VF453_18240"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEX7689651.1"
                     /translation="MTDYFASTPAIRYAGSGSADPLSFRWYDKDRLVLGKRMEDQLRF
                     AVCYWHTFCWNGFDPFGYDGTFERPWQRIADPMEAARAKADAAFDFFAKLGAPFYCFH
                     DRDVAPEGATPRESVDNFRRMVDVLGEKQQATGVKLLWGTANLFSHRRYMSGAATNPN
                     PEIFALAALQVKEAMDATRRLGGENYVLWGGREGYETLLNTDLARESRQYGRFLQMAV
                     EHKHRIGLPGHILIEPKPREPTKHQYDYDVATVHGFLRQYGLEGEVKVNIEANHATLS
                     GHSFEHEIATAFALGLFGSLDMNRGDMQCGWDTDQFPNNLADTALALYHVLRGGGFTH
                     GGLNFDAKVRRQSIDAEDLFHGHVGAMDLCARALLVAERMVADGRLAGLVDARYAAWD
                     GEVGRKLLAEGATLEDVARHALERNVDERPVSGRQEALENLVNEYL"
BASE COUNT          854 a         2076 c         2187 g          999 t
ORIGIN      
        1 gcgcgcgcgc gagcctgaaa gcgggcgcgc ccgccgggca ggggcgggcg cgagccggtc
       61 gcagcgcgtc gcgggcggcc ccgcgacgtc acatgccgga gtagttcggg ccgccgccgc
      121 cctcgggcgt gacccagacg atgttctgcg tcggatcctt gatgtcgcag gtcttgcagt
      181 gcacgcagtt ctgcgcgttg atctgcaggc ggtcgctgcc gtcctcgttc ttcacgaact
      241 cgtagacgcc cgccgggcag tagcggctct ccggccccgc gtattcggcc aggttgacct
      301 tcacgggcac gcccccgtcc ttgagcgtca ggtggaccgg ctggtcctcg gcgtggttgg
      361 tgttgctcac gaagaccgag ctcaggcggt cgaaggtgag cttgccgtcg ggcttcgggt
      421 aggcgatctg cggcatctcg gacgcggggc gcagggcctc gttgtccttc ttggtgttgt
      481 gcagcgtcca gggcgggatg ggcatgccca gcttgggcat gagccactgc tcgaggccgg
      541 tcatcacgtt gccgaccagc ttgcccttct tgaaccagag cttgaagttc ttcgtgcgct
      601 gcagttcctt gtgcagccag ctgcgctcga atgcggcggg gtaggccgag agctcgtcgg
      661 cctggcggcc ggcctgcagg gcctcgaacg cggcctcggc ggccatcatg ccgctcttga
      721 tcgcggcgtg gctgcccttg atgcgcgcgg cgtccagcgt gccggcatcg cagcccacca
      781 gcgcgccgcc cgggaagacc atcttgggca gcgactgcag gccgccgttg ttgatcgccc
      841 gcgcgccgta ggacagccgc ttgccgcctt cgaggtgcgc gcgcacggcg gggtgcgtct
      901 tccagcgctg catctcctcg aacgggctca tccacgggtt ctggtagtcg aggccgatga
      961 cgaagcccag ggtcaccttg ttgtccgcca ggtggtagag gaagccgccg ccgaaggtcg
     1021 tggcgtcggt gatcggccag ccggcggtgt gcaccacgag cccgggcttg gccttgtcgg
     1081 cggggatctc ccacagctcc ttgatgccga tcgcccagct ctgcgcgtcg cggccttcct
     1141 gcagccggta cttcgcgagc agctgcttgc cgaggtggcc gcgcgcgcct tcggcgaaga
     1201 tcgtgtactt gccgtgcagc tccatgccga gctggaagcc gtcgtgcggc tcgccgtcgc
     1261 ggcccacgcc cacgttgccc gtggccacgc ccttgaccgc gccctggtcg tcgtacagga
     1321 cttcggccgc ggcgaagccc gggaagatct cgacgcccag cgcctcggcc tggccggcca
     1381 gccacttgac gacgtcgctc aggctgatga cgtagttgcc gtggttgtgg aacgggtagg
     1441 gcatcagcca ttcgggcacc gacttcgagc cggtctccga caggaacagg aactcgtcgc
     1501 cggtgaccgg ctggttcagc ggcgcgccgt cggcctgcca gttcggcagc agctcggtga
     1561 tcgccttcgg atccatgacc gcgccggaca ggatgtgcgc gccgggctcc gatccctttt
     1621 ccagcacgac caccgagacc tccttgccgg cctcggccgc cagctgcttg aggcggatcg
     1681 cggtcgcgag gccggcgggg ccggcgccga cgatcacgac gtcgtattcc atcgattcgc
     1741 gcgggccgta ctgggcgagg agttcttgcg gcgtcatggt gggcagtctt tcctggaagg
     1801 ctgtcgaggt gggaggcgcc tgcgggcggg gcgggctgcg cgcattctac gagtcctccg
     1861 gaataaaaaa gcacgatcgt tcgcattttc tgcgcgtgct atcgtggaac gcatcacagg
     1921 agcaacatcc accatgggta tgaaaatcga cctggcgggc cgcgtggcgc tcgtcacggg
     1981 cgcgtcgagc gggctcggcg cgcagttcgc acgaaccctg gccgagtgcg gtgcgggcgt
     2041 cgtgctcgcc gcacgccggc tggaacggct gaagacgctg cgcgccgaga tcgaggccaa
     2101 cggcggcgac gcccacgtcg tcgagctcga cgtcaccgat cccgacagca tcaaggccgc
     2161 ggtcgcccac gccgagaccg agatgggcgt catcgacatc ctcgtcaaca actccggcgt
     2221 ctcgacgacg cagaagctca ccgacgtgac gcccgaggac ttcgactacg tgttcgacgt
     2281 caacacgcgc ggcgcgttct tcgtcgccca ggaagtcgcc aagcgcatga tcgcccgcgc
     2341 gcgaggcgcc gctccgggca cctataccgg tgggcgtatc gtcaacgtcg cctccatggc
     2401 gggcctgcgg ccgctgtcgc agatcggcgt ctacgcgatg agcaaggccg ccgtcgtcca
     2461 catgacgcgc gcgatggcgc tcgaatgggg gcgctacgac atcaacgtca acgcgctgtg
     2521 ccccggctac atcgacaccg agatcaacca ccaccactgg tcgaccgagg ccggccagaa
     2581 gctggtgcag atgaccccgc gcaagcgcat cggcaagccg accgacctcg acagcgcgct
     2641 gctgatgctg tgcgccaacg agagccattt catcaacggc gccgtgctgc aggccgacga
     2701 cggcttcggg atctgacggc cccgcgtccg cgacatgagg ctggaactgc cggacgggct
     2761 caggctcgtc cacgagatgc gcatccccgt gcgctggggc gacctggacg cgatggggca
     2821 cgtcaacaac acgatctact tccgctactt cgagtcgctg cgcatcgact ggctgcgggc
     2881 cttcggcgcg gagccgaacc cggcgggcgt ggggccggtg atggccaacg gcttctgcaa
     2941 cttcctgcgc cagatcgagt acccggggga gatcgtcgcg cgccacttcg tcggcgcggt
     3001 gggcaccaag agcgtggaca cctatttcac gctgtccatg gccgacgacc ccgacacggt
     3061 ctgcgcgaac ggcggcgcga cgctcgtctg gctggacttc ccgcgcaaga cgaccgtgcc
     3121 gctgcccgac gacctgcgcg ccgcctgcac ggcctgagcc ggccgcggcc tatcgctgcg
     3181 ccgcgtccgc ggcgtgttcg cccggcgcgc ccacgtggtc cttgcgccgc acgacgctgg
     3241 acagcttcgc gtcctcggga tagtagaagc ggatctcgcc gccgtgccac acgcccagcc
     3301 agagcatgtt ctggctgatc gcgtcgtggt cggtcgcgtc gaacgggtgg tcgtaggtcg
     3361 tcacgacgcc ctcgatcggc cgcggcaagg tctccagcgc ggccttcagc gcggggccgg
     3421 agacgtcgcc atgggtctgg aacagcgcgg ccagcatcag gtacacggcg tcgtagctct
     3481 gcgcggccgc catcagcgag ctgatcggcc ggtcgttcga catcttgaag tacttggcga
     3541 ggaagccggc acggcgctcg ttgtagatgt cgtagatgac ggtctgcacc atcatcgtcc
     3601 cctcgagcag gtcgggcccg cccagctcga ggacgctgcg cgccgacagc gcccacgtcg
     3661 acagcagcgg cagcttcacg tgcgcggcgc ggcggccctt gagcaccgtg acctggtcgg
     3721 ggccgacggc gtagacgacg atcgcctgcg cgcccgactg cgcggcctgc tccatctccg
     3781 aggcgagcga ctcgacgccc ggcgcgaagc gcgcgacgta cgcgggctgc aggccgcgcg
     3841 cggccagttc ggccgtgacg tccttcaggc cgccctcgcc gtagccggtc ttgtccgcga
     3901 agatcgcgac cttcttcagg cggcgccgct cgacgatctc ggagacgagg aacttcgcgt
     3961 tcagcaggtc gtacggcgcg ttgcggaaga tgtagctgtc cgcggccgcg aactgcctgg
     4021 tgacggccgt gccctgcgag caggggatca tcagcaggct cctgctggcc tggaagacct
     4081 tggcggcctg cagcgcgacg cccgtgttgc agtagccgat cgtgaagtcc accttctcct
     4141 tgtcgaccag ttcgtgcgcg atctgctcgc cgacctgcgg gacgctcttg tcgttgcgct
     4201 cgaccagctt cagcggccgg cccaggtagc cgccggcctc gttgatctcc tgcaccgcga
     4261 gctcggcgcc gaacttcgtg ctcgccccga gatcctcgct tccgccggtc agcggcgcga
     4321 ccaggccgat gcgcagcggc gcgggctccg catatgacgg cgccggggtg gcgacgcaca
     4381 ggccgaggca cagaccgagg aacgtccagc gcggcttccg gcgcagggtc atgtttgtct
     4441 ccaggttggc gcggcaccgt ggccttcagg ggcggcggcg cagtctattg ctggagtcga
     4501 ctctagccga gcttgcgacg ggcctgcctc cccgagaact cgtaggcggc agtccttgtc
     4561 ggcgcattag ttcacgcggg cggcggatga tcgcatggcg gcggcgcggg accgcggggc
     4621 ggccctgccg cgtcaaaggt attcgttgac gaggttctcc agcgcctcct ggcgccccga
     4681 gaccgggcgc tcgtcgacgt tgcgctcgag cgcgtgccgc gcgacgtcct ccagcgtggc
     4741 gccctcggcg agcagcttgc ggccgacctc gccgtcccac gcggcatagc gcgcgtcgac
     4801 gaggccggcg aggcggccgt cggccaccat gcgctcggcg accagcaggg cccgcgcgca
     4861 caggtccatc gcaccgacgt ggccgtggaa caggtcctcg gcgtcgatcg actgccggcg
     4921 caccttggcg tcgaagttca ggccgccgtg cgtgaagccg ccgccgcgca gcacgtggta
     4981 cagcgcgagc gcggtgtcgg cgaggttgtt ggggaactgg tcggtgtccc agccgcactg
     5041 catgtcgccg cggttcatgt ccagcgagcc gaagaggccg agcgcgaacg ccgtggcgat
     5101 ctcgtgctcg aagctgtgtc ccgacagcgt cgcgtggttg gcctcgatgt tgaccttgac
     5161 ctcgccctcc aggccgtact ggcgcaggaa gccgtgcacc gtcgcgacgt cgtagtcgta
     5221 ctggtgcttg gtcggctcgc gcggcttggg ctcgatgagg atgtggccgg gcaggccgat
     5281 gcggtgcttg tgctccacgg ccatctgcag gaagcggccg tactggcgcg actcgcgcgc
     5341 gaggtcggtg ttgagcaggg tctcgtagcc ttcgcggccg ccccacagca cgtagttctc
     5401 gccgccgagc ctgcgcgtcg cgtccatcgc ctccttcacc tgcagcgcgg cgagcgcgaa
     5461 gatctcgggg ttgggattgg tggccgcgcc gctcatgtag cgccggtggc tgaacaggtt
     5521 ggccgtgccc cacagcagct tcacgccggt ggcctgctgc ttctcgccga ggacgtcgac
     5581 catgcggcgg aagttgtcga cgctctcgcg cggggtcgcg ccctcggggg cgacgtcgcg
     5641 gtcgtggaag cagtagaacg gcgcgccgag cttcgcgaag aagtcgaacg cggcgtcggc
     5701 cttggcgcgg gcggcctcca tcgggtcggc gatgcgctgc caggggcgct cgaaggtgcc
     5761 gtcgtagccg aacgggtcga agccgttcca gcagaacgtg tgccagtagc agaccgcgaa
     5821 gcgcagctgg tcctccatgc gcttgccgag gacgaggcgg tccttgtcgt accagcggaa
     5881 gctcagcgga tcggcggagc cggacccggc gtagcggatc gcgggcgtgg aggcgaagta
     5941 gtcggtcatg gtcgtggtcg tccctggagg tggcgcatcc ggcgcggatg cgtgtacggg
     6001 ttcgggtaga ggtcagtcgc gcgggttcag cgcgcgcgtc gcgaggtaga gcgcccggaa
     6061 gcgcgcgagg cgcggcgcga gggcggcgtg gcgggcgagg tcgggctcga agcgcg
//
LOCUS       DASVLN010000768         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_309399, whole genome shotgun sequence.
ACCESSION   DASVLN010000768 DASVLN010000000
VERSION     DASVLN010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309399"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2200)
                     /locus_tag="VF453_18245"
     CDS             complement(<1..>2200)
                     /locus_tag="VF453_18245"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX7689652.1"
                     /translation="LEALRGGFVPAGRINSAAGTDLTATLINCFVQPVGDSIAQLEDG
                     VPGIYTALTEAAETMRRGGGVGYDFSRIRPQGAWVGSTRSRASGPVSYMRVFDRSCET
                     VESAGSRRGAQMAVLRCDHPDVEAFIHAKDGGDLTNFNISVGVSDAFMQAVEDDTTFD
                     LVHRAQPSEEQIAAGARQRPDGLWIYRSPRARALWDQIMRCTYDHAEPGVLFLDRINQ
                     DNNLSYCETISATNPCGEQPLPPYGCCCLGSIDLTRFVRDPFEPQARFDVEAFAALVR
                     TSVRMLDNVLDITVWPLPAQNDEANAKRRVGLGFTGLGDALVMLGLRYDTPAARDEAQ
                     RISQAMRDAAYDASADLATERGAFPLFNADLYLSRGTFASRLPAALKDKIRAQGLRNS
                     HLLSIAPTGTISLAFADNASNGIEPPFSWTYTRRKRMADGGAKEYAVEDHAWRLYRHL
                     KGADAPLTDAFVTALEMSAESHAAMVATVAPYVDTSISKTVNVPADYPYADFQGLYRF
                     AWKSGLKGLATYRPNAVLGSVLSTTPAAPEPLPNAATSETPQPLVAEDAARAIDAAAS
                     PADQRLTLDRPATAVMNSLRWPGRPEMNGGNPAWTYMIRHPHGEFALFVGEMPAEAGP
                     GFGLFAQNVPFEVWVNGAEQPRGLGALAKTLSMDMRVNDAQWLRLKLDVLATVAEERA
                     FDMPFPPAGEARLFPGVVAATAAVIRWRCEQLGALADGGPTPMLDAMSCSQ"
BASE COUNT          290 a          751 c          844 g          315 t
ORIGIN      
        1 ctgcgagcag ctcatcgcgt cgagcatggg cgtcgggccg ccgtcggcga gcgcgccgag
       61 ctgctcgcag cgccagcgga tgacggccgc cgtcgcggcg acgacgccgg ggaacagccg
      121 cgcctcgccc gcgggcggga acggcatgtc gaacgcgcgc tcctcggcga cggtggcgag
      181 cacgtcgagc ttgagccgga gccactgggc atcgttgacg cgcatgtcca tcgacagcgt
      241 cttggcgagc gcgcccaggc cgcgcggctg ctcggcgccg ttgacccaga cctcgaaggg
      301 cacgttctgc gcgaacagcc cgaagccggg gccggcctcg gccggcatct cgccgacgaa
      361 cagggcgaac tcgccatgcg ggtggcggat catgtacgtc caggccgggt tgccgccgtt
      421 catctcgggc cggccgggcc agcgcagcga gttcatgacg gcggtcgcgg ggcggtcgag
      481 cgtgaggcgc tggtcggccg ggctcgcggc ggcgtcgatc gcgcgcgccg cgtcctcggc
      541 gaccagcggc tgcggcgtct ccgaggtcgc cgcgttgggc agcggctcgg gcgccgcggg
      601 cgtcgtgctc agcaccgagc ccagcaccgc gttcggccgg taggtcgcca ggcccttgag
      661 ccccgacttc cacgcgaagc ggtacaggcc ctggaagtcc gcatagggat agtcggccgg
      721 gacgttgacc gtcttgctga tcgaggtgtc gacgtagggc gcgacggtcg cgaccatcgc
      781 cgcgtggctc tcggcgctca tctccagcgc ggtgacgaag gcgtcggtga gcggcgcgtc
      841 ggcgcccttg aggtggcggt agaggcgcca ggcgtgatcc tcgaccgcgt actccttcgc
      901 gccgccgtcg gccatgcgct tgcggcgcgt gtacgtccac gagaacggcg gctcgatgcc
      961 gttgctcgcg ttgtcggcga aggccaggct gatcgtgccc gtcggcgcga tcgacagcag
     1021 gtgcgagttg cgcagcccct gcgcgcggat cttgtccttc agcgccgcgg gcaggcgcga
     1081 cgcgaaggtg ccgcgcgaca ggtacaggtc ggcattgaac agcgggaacg cgccgcgctc
     1141 ggtcgcgagg tcggcggagg cgtcgtaggc cgcgtcgcgc atcgcctgcg agatgcgctg
     1201 cgcctcgtcg cgggcggcgg gcgtgtcgta gcgcaggccc agcatcacga gcgcgtcgcc
     1261 cagccccgtg aagccgaggc ccacgcgccg cttcgcgttc gcctcgtcgt tctgcgccgg
     1321 cagcggccag acggtgatgt ccagcacgtt gtccagcatc cgcaccgacg tgcgcaccag
     1381 cgcggcgaac gcctcgacgt cgaagcgcgc ttgcggctcg aacgggtcgc gcacgaagcg
     1441 ggtcaggtcg atggagccga ggcagcagca gccgtagggc ggaagcggct gctcgccgca
     1501 cgggttggtc gcgctgatcg tctcgcaata gctcaggttg ttgtcctggt tgatgcgatc
     1561 gaggaacagc acgcccggct cggcgtggtc gtaggtgcag cgcatgatct ggtcccacag
     1621 cgcgcgggcg cgcgggctgc ggtagatcca caggccgtcg gggcgctggc gggcgccggc
     1681 ggcgatctgc tcctcgctcg gctgcgcgcg gtggacgagg tcgaacgtgg tgtcgtcctc
     1741 gacggcctgc atgaaggcgt cgctcacgcc caccgagatg ttgaagttgg tgaggtcgcc
     1801 gccgtccttg gcgtggatga aggcctcgac gtcgggatgg tcgcagcgca gcacggccat
     1861 ctgcgcgccg cggcggctgc cggccgattc gaccgtctcg cagctgcggt cgaacacgcg
     1921 catgtagctc accggaccgg aggcgcggct gcgcgtcgag ccgacccacg cgccctgcgg
     1981 acggatgcgc gagaagtcgt agccgacgcc gccgccgcgg cgcatcgtct cggcggcctc
     2041 ggtgagcgcc gtgtagatgc cgggcacgcc gtcctcgagc tgcgcgatcg agtcgcccac
     2101 gggctgcacg aagcagttga tcagcgtcgc ggtgagatcg gtgccggccg ccgagttgat
     2161 gcgacccgcg ggcacgaagc cgccgcgcag cgcctcgagg
//
LOCUS       DASVLN010000769         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_525039, whole genome shotgun sequence.
ACCESSION   DASVLN010000769 DASVLN010000000
VERSION     DASVLN010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525039"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VF453_18250"
     CDS             complement(<1..1260)
                     /locus_tag="VF453_18250"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7689653.1"
                     /translation="MADAADVVAGPPGKARVSVVDKTVWARLSPLLDEFLDLPEPEHA
                     ARLAQVRAQDPALADALAEMLGREQAIEEAGFLEAPAVPRPAGLEGQAVGAYTLVREL
                     GHGGMGTVWLARRTDGRYQGEVAIKFLRSGLFGHGDAGRFEREGSILARLSHPNIARL
                     LDAGVTSDGTQPYLVLEYIDGEPVDAYCNRLALPVAARLRLFLDVLGAVAHAHNRLIL
                     HRDLKPSNILVTASGEVKLLDFGIAKLLDDTAAGASAVTAHAGNAFTPDFAAPEQLQG
                     GDVTTATDVYALGVLMYVLLGGEHPTTSPTGAPLDRMRAVIEVEPKRLSESVLRRGGP
                     RSRHSTASRKLAAELSGDVDTIAAKALKKSSAERYANAADLADDVRRYLAHEPIAARP
                     DARLYRVAKFVRRHRAGVATASVAVLAL"
     gene            complement(1253..1807)
                     /locus_tag="VF453_18255"
     CDS             complement(1253..1807)
                     /locus_tag="VF453_18255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX7689654.1"
                     /translation="MSEITTLLKAAGAGDRLAADQAFALLYADLQRLARSRMRQSNGV
                     TQLDTTALVHESYLRFQGAREASFADREHFLGYASRVMRAVVVDFVRARTAERRGGGA
                     AHVTLNTAISDAVPSTDEEILRVHEALEELEGTEARLARIVEMKYFAGLSDAEIAAEL
                     QINERTVQRDWRKARLFLAAVLNG"
     gene            2084..2611
                     /locus_tag="VF453_18260"
     CDS             2084..2611
                     /locus_tag="VF453_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689655.1"
                     /translation="MLKEIDVRIPPRLERERLARQAPVDRARGPAVPTRVEGIAEVAS
                     IVDRHLAQCRRHRSLLALVCVGVDVVDTESGLGLVPEEVERRVHEEVINRVCARVRGS
                     DTVLCPNERDSCVLLPGTSAEALRRVGARLRQALDGDYRIDGTLVRVSVRIGSAAHPD
                     DGAQAPELLRRATGW"
BASE COUNT          329 a          920 c          994 g          374 t
ORIGIN      
        1 cagcgccagc acggcgacgc tcgcggtcgc gacgcccgcg cggtggcggc gcacgaactt
       61 ggcgacgcgg tacaggcgcg cgtccggccg ggcggcgatc ggctcgtgcg cgagatagcg
      121 ccgcacgtcg tcggcgaggt cggcggcgtt ggcgtagcgc tcggcggagg acttcttgag
      181 cgccttggcg gcgatcgtgt cgacgtcgcc gctcagctcc gcggccagct tgcgcgaggc
      241 cgtcgagtgc cggctgcgcg gcccgccgcg ccgcagcacc gactccgaca gccgcttcgg
      301 ctcgacctcg atcacggcac gcatgcgatc cagcggcgcg ccggtgggcg aggtcgtcgg
      361 atgctcgccg ccgagcagca cgtacatcag cacgcccagc gcgtagacgt cggtggcggt
      421 cgtgacgtcg ccgccctgca gctgctcggg tgccgcgaag tcgggcgtga aggcgttgcc
      481 ggcgtgcgcg gtgacggcgc tcgcgccggc ggcggtgtcg tcgagcagct tggcgatgcc
      541 gaagtccagc agcttcacct cgccggatgc ggtgacgagg atgttcgacg gcttcaggtc
      601 gcggtgcagg atcagccggt tgtgcgcatg cgcgaccgcg ccgagcacgt cgaggaacag
      661 ccgcagccgc gccgccaccg gcagcgcgag gcggttgcag taggcgtcga ccggctcgcc
      721 gtcgatgtac tcgagcacca ggtagggctg cgtgccgtcc gacgtgacgc ccgcgtccag
      781 caggcgcgcg atgttcgggt gcgacaggcg cgcgaggatg ctgccctcgc gctcgaagcg
      841 gccggcgtcg ccgtggccga agaggccgga gcgcaggaac ttgatcgcca cctcgccctg
      901 gtagcggccg tcggtgcggc gcgccagcca gaccgtgccc atgccgccgt gcccgagctc
      961 gcgcaccagc gtgtaggcgc ccacggcctg gccctcgagg ccggccggtc gcggcacggc
     1021 gggcgcctcg aggaagccgg cctcctcgat cgcctgctcg cgcccgagca tctcggccag
     1081 cgcgtcggcg agcgcgggat cctgcgcccg cacctgcgcg agccgcgccg cgtgctccgg
     1141 ctcgggcagg tcgaggaact cgtcgagcag cggcgagagc cgtgcccaca ccgtcttgtc
     1201 gacgacgctc acgcgcgcct tgcccggcgg cccggcgacg acgtccgccg cgtcagccat
     1261 tgagcaccgc ggcgaggaac agccgcgcct tgcgccagtc gcgctggacg gtgcgctcgt
     1321 tgatctgcag ctcggccgcg atctcggcgt ccgacaggcc ggcgaagtac ttcatctcga
     1381 cgatgcgggc gaggcgggcc tccgtgccct cgagctcctc cagggcctcg tgcacgcgca
     1441 ggatctcctc gtccgtgctc gggacggcgt cgctgatggc ggtgttcagc gtcacgtggg
     1501 cggcgccgcc gccgcggcgc tcggcggtgc gggcgcggac gaagtcgacg acgaccgcgc
     1561 gcatcacgcg cgatgcgtac ccgaggaaat gctcgcgatc cgcgaacgac gcctcgcgcg
     1621 cgccctggaa gcgcaggtag gactcgtgca cgagggcggt cgtgtcgagc tgcgtgacgc
     1681 cgttggactg gcgcatccgg ctgcgggcca ggcgctggag gtcggcgtac agcagcgcga
     1741 acgcctggtc ggcggcgagg cgatcgccgg cgccggcggc cttcaggagg gtcgtgatct
     1801 ccgacatgga tgcgtcaaat ggggaggctt cgccgtgctc gaagcgagac tctagcgcgg
     1861 cggcgagaaa tattttgttt cggatgtcgg gtttcgtgtg gagtttccgt tttggaggaa
     1921 gaggggcggt ttttcggacg gaaagggagg cctggagggc caaccggggt tccgtccctc
     1981 acctttgccc gcggctccca agccggtgtg cgccttccgg ggttcgcgcc gtgcgccgcg
     2041 ggccttcctt tttccagcgt cctcgacgcg ccgaggtgtg gccatgctga aggaaatcga
     2101 tgtcaggatt cccccgcggc tcgagcggga acgccttgcc cggcaggcgc cggtcgaccg
     2161 cgcccgcggg ccggccgtgc ccacgcgcgt cgagggcatc gccgaggtgg cgagcatcgt
     2221 cgaccgccac ctggcgcagt gccgccgcca ccgcagcctg ctcgcgctgg tgtgcgtggg
     2281 cgtggatgtc gtcgacaccg agtccggcct gggcctggtt cccgaggagg tcgagcgccg
     2341 cgtccacgag gaagtgatca accgcgtgtg cgctcgcgtc cgcggcagcg acaccgtcct
     2401 gtgcccgaac gagcgcgatt cctgcgtgct gctgccgggc acgtccgccg aggcgctcag
     2461 gcgcgtcggc gcgcgcctgc gccaggcgct cgacggcgac taccgcatcg acggcacgct
     2521 ggtgcgcgtc tcggtgcgca tcggcagcgc cgcccatccg gacgacggcg cccaggcgcc
     2581 cgagctgctg cgacgcgcga ccggctggtg accggcg
//
LOCUS       DASVLN010000770         3493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_362132, whole genome shotgun sequence.
ACCESSION   DASVLN010000770 DASVLN010000000
VERSION     DASVLN010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362132"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VF453_18265"
     CDS             <1..685
                     /locus_tag="VF453_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HEX7689656.1"
                     /translation="AVLGAAGGQGLEGLAGVPIAALDAWHPCWGRLPPDAYLLDGGKY
                     RQRRHGLFVVDGDDVVAAPHRPHWQPVDYNALHGGIERHFEPLEPALAQDPAWKRLLA
                     ALAARASALKGAQRWYVEAHLFRIDTASGIGRPTPEGAHRDGVDLVAVTLVGRHGIKG
                     GETRVFQAQAAAGLRFTLSEPWTTVLLDDARVIHETTPIQPLKAGEPGWRDTLVLTFR
                     AGGFQGPAL"
     gene            792..2291
                     /locus_tag="VF453_18270"
     CDS             792..2291
                     /locus_tag="VF453_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX7689657.1"
                     /translation="MSESTASTDKPKTSLRDAVERALAAAPARSTGSIALGGRRLDYR
                     VEGAFVPVVATALDDRRGEPEAAVFTTAYLLERAPGEATPRPVCFAFNGGPGAASIWL
                     HLGALGPKRVPIEDDGTMPPPPWSVEDNPLSWFEHFDLVFIDPPHTGYSITAGDDARK
                     RMLSVDGDVACLVDVIRGWLSRHARWTSPLYLAGESYGTTRGAAIADKLAEQGVALSG
                     VILVSCAMDIQALEFTPRNDLPYALYLPAFAGVAQYHGCLQGPQAESGAAARALAAEF
                     VEAEYLRLLHRGGTLTHDQRARAARRIGELAGLAPALVEQRNLRIADNLFFTELLRER
                     GQVVGRLEARVAGPMPLAQTHEWDFDPGLEAIHGPYGMAAMGYFAETLGFDRGWRYEL
                     MSHEVHEQWNWGRGKALGNSFCCTSPDLSRALRRLPHLRVFVASGHYDLGTPSTASDW
                     SLAQLDIPDDVRRRVTHRYYDAGHMMYTRQADLAKLKGDLDEWLSAGAA"
     regulatory      2395..2550
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            2686..3213
                     /locus_tag="VF453_18275"
     CDS             2686..3213
                     /locus_tag="VF453_18275"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7689658.1"
                     /translation="MNQIPATSLHAVPAASPAAADAALRPAIAVISASWHADIVGQAR
                     RALLAELERGPAPPRRVDCFDVPGAFEIPLLARRLAASGRYDAIVACALVVNGGIYRH
                     EFVATAVIDGLMRAQLDTDVPVLSCVLTPRDFHEHEEHQRFFSEHFVKKGVEVARACM
                     AIVAGLRAARLSMAA"
BASE COUNT          451 a         1342 c         1250 g          450 t
ORIGIN      
        1 cgcggtgctg ggcgccgccg gcggccaggg cctcgagggc ctggcgggcg tgccgatcgc
       61 ggccctggac gcctggcatc cctgctgggg ccggctgccg cccgatgcct acctgctcga
      121 tggcggcaag taccgccagc gccgccacgg cctgttcgtc gtcgacggcg acgacgtggt
      181 cgcggcgccg caccggccgc actggcagcc ggtcgactac aacgccctgc acggcggcat
      241 cgagcgccac ttcgagccgc tggagcccgc cctcgcgcag gatccggcgt ggaagcggct
      301 gctggcggcg ctcgcggccc gggcctcggc gctcaagggc gcgcagcgct ggtacgtcga
      361 ggcgcacctg ttccgcatcg acaccgccag cggcatcggc cgcccgacgc ccgagggcgc
      421 gcaccgcgac ggcgtcgacc tggtcgcggt gacgctcgtc ggccgccacg gcatcaaggg
      481 cggcgaaacg cgcgtcttcc aggcccaggc ggccgccggc ctgcgcttca cgctgtccga
      541 gccgtggacg acggtgctgc tcgacgacgc ccgcgtgatc cacgagacga cgccgatcca
      601 gccgctcaag gccggcgagc cgggctggcg cgacacgctg gtgctgacct tccgggcggg
      661 cgggttccag ggccccgcgc tatgaccggc ccgtgcccgg cccagggatc ggcggccgcc
      721 cggggcccga agggaagggt gacaatgcgc gccttcccgg caccccggcc gcgatccggc
      781 cgccaccgac catgagcgaa tccaccgcgt cgaccgacaa gccgaagacc tcgctgaggg
      841 acgccgtcga gcgcgcgctc gccgcggcgc cggcgcgcag caccggctcg atcgcgctgg
      901 gcggccggcg gctcgactac cgcgtcgagg gcgcgttcgt gcccgtggtc gccacggcgc
      961 tcgacgatcg ccgcggcgag cccgaggccg ccgtcttcac gaccgcctac ctgctggaac
     1021 gcgcgcccgg cgaggcgacg ccaaggccgg tctgcttcgc gttcaacggc gggccgggcg
     1081 cggcctcgat ctggctgcac ctgggcgcgc tgggaccgaa gcgcgtgccc atcgaggacg
     1141 acggcacgat gccgccgccg ccgtggtcgg tcgaggacaa cccgctcagc tggttcgagc
     1201 acttcgacct cgtcttcatc gatccgccgc acaccggcta ctcgatcacc gcgggcgacg
     1261 acgcgcgcaa gcgcatgctg tccgtcgacg gcgacgtcgc ctgcctcgtg gacgtcatcc
     1321 gcggctggct ctcgcgccac gcgcgctgga cgtcgccgct gtacctcgcc ggcgagagct
     1381 acggcacgac gcgcggcgcg gccattgccg acaagctcgc cgagcagggg gtggcgctct
     1441 cgggcgtcat cctcgtgtcg tgcgcgatgg acatccaggc gctcgagttc acgccgcgca
     1501 acgacctgcc gtacgcgttg tacctgccgg ccttcgcggg cgtcgcgcag taccacggct
     1561 gcctgcaggg gccgcaggcc gagtcgggcg ccgcggcgcg cgcgctcgcc gccgagttcg
     1621 tcgaggccga gtacctgcgc ctgctccacc gcggcggcac actgacgcac gaccagcgtg
     1681 cccgcgcggc gcgccgcatc ggcgagctcg cgggactcgc gccggcgctc gtcgagcagc
     1741 gcaacctgcg catcgccgac aacctcttct tcaccgagct gctgcgcgag cgcggccagg
     1801 tcgtcggccg gctggaggcg cgcgtcgccg ggccgatgcc gctcgcacag acgcacgagt
     1861 gggacttcga ccccggcctg gaggcgatcc acggccccta cggcatggcg gcgatgggct
     1921 acttcgccga gacgctgggc ttcgaccgcg gctggcgcta cgagctgatg tcccacgagg
     1981 tgcacgagca gtggaactgg ggccgcggca aggcgctggg caactccttc tgctgcacga
     2041 gccccgacct ctcgcgcgcg ctgcgccgcc tgccgcacct gcgcgtgttc gtcgccagcg
     2101 gccactacga cctcggcacc ccctcgaccg cgagcgactg gtcgctcgcg cagctcgaca
     2161 tccccgacga cgtgcgccgg cgcgtgacgc accgctacta cgacgccggc cacatgatgt
     2221 acacgcgcca ggcggatctc gcgaagctca agggcgacct ggacgagtgg ctgtccgcgg
     2281 gcgcggcctg aggccgcggc ccggcgccgg cccttcgcgc gcgtgtcgcg cacgtggcag
     2341 ccgccgcacc tcgcctgcat agaatcgtcc gcgccgaccc cacccggggc cggcatgcgt
     2401 tctcagggcg gggtggaagt ccccaccggc ggtatccgcg actcgtcgcg ggagcccgcg
     2461 agcgcccgcg cgtcgcccga tgcgcggggt cagcagacct ggtgagaggc cagggccgac
     2521 ggtcacagtc cggatgaaag agaaacgcga cgccgcggcg gctgcatgcg ccgcgcgtgc
     2581 ccgcgcgcgt gcgtgacgcg cgcgacgggc ccgcgcacgc aggcgatgcc gcgcgtctcg
     2641 tgcgcgccct gattctggcc cacccctctt cactggagag acaccatgaa tcagatccct
     2701 gccacttccc tccacgccgt tcccgcggcg tccccggccg ccgccgacgc ggccctgcgg
     2761 cccgcgatcg cggtgatcag cgcctcctgg cacgccgaca tcgtcggcca ggcgcggcgc
     2821 gcgctgctgg ccgagctcga gcgcggcccc gcgccgccgc ggcgcgtcga ctgcttcgac
     2881 gtccccggcg cgttcgagat cccgctgctc gcgcggcgcc tcgccgcctc gggacgctac
     2941 gacgcgatcg tcgcctgcgc gctggtcgtc aatggcggga tctatcgcca cgagttcgtc
     3001 gcgaccgcgg tcatcgacgg cctcatgcgc gcccagctcg acaccgacgt gccggtgctc
     3061 tcgtgcgtgc tgacgccgcg cgacttccac gagcacgagg agcaccagcg cttcttcagc
     3121 gagcacttcg tcaagaaggg cgtcgaggtg gcgcgggcgt gcatggcgat cgtcgcgggc
     3181 ctgcgggcgg cgcggctgtc gatggcggcc tgagcgcccg tcggtcgctc ggccgatgac
     3241 tcccgaagtt gttcgacctg gaaggggggc ggatcggagt cctgcgccga cggcgcgcgc
     3301 cgcatgatcg caagatccgt caggcgaacc gcaacctggt gcaggctgcg cccacacacg
     3361 ggtcgccaga atggctccgt tcaagaacca cgacggagac aacatgaagt ccatcggacg
     3421 gccagcgact ttcctcgtcg cgagcgcctg cgccctcgcg ctggcggcgt gcagcggcct
     3481 gcctggcggg cag
//
LOCUS       DASVLN010000771         5389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_39101, whole genome shotgun sequence.
ACCESSION   DASVLN010000771 DASVLN010000000
VERSION     DASVLN010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39101"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /gene="dnaA"
                     /locus_tag="VF453_18280"
     CDS             <1..1090
                     /gene="dnaA"
                     /locus_tag="VF453_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7689659.1"
                     /translation="VVDEAPPGARPAARPMHAAPALNTHRLNPALTFDTLVAGRANQM
                     ARTAALHVAGAPGAMYNPLFVYGGVGLGKTHLVHAVGNQLLRDKPDARVLYLHAEQFI
                     SDVVKNYQRKTFDELKAKYHSLDLLLIDDVQFFAGKERTQEEFFNAFEALLAKRAHII
                     MTSDTYPKGLADIDERLTSRFDSGLTVAIEPPELEMRVAILIRKAEAEGTQMPEEVAF
                     FIAKNVRANVRELEGALRKVLAYSRFSQKEINIALAREALKDLLSIQNRQISVENIQK
                     TVADFYKIKVADMYSKKRPASIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLH
                     AVRKIGGERQKNTELNQELHVLEQTLKG"
     gene            1273..2382
                     /gene="dnaN"
                     /locus_tag="VF453_18285"
     CDS             1273..2382
                     /gene="dnaN"
                     /locus_tag="VF453_18285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX7689660.1"
                     /translation="MIVLKAPQEKLLGALQAVSGIVERRHTLPILANVLMRKNGESIE
                     FTTSDLEIQVRTSAVLGGDEGSLSTTVGSRKMIDILKSLPADQIVTLTSSGNKLTLQA
                     GKSRFTMQTLPADDFPLVNEAVDFGPSFGVPQKVLKSLIDQVHFAMAVHDIRYYLNGI
                     LFVAEGKTLTLVATDGHRLGLAQATLETEIPKQEVILPRKTVLELARLLNATTDEPIE
                     MRFAGNQAKFTFSGLEFVTKLVEGKFPDYNRVIPKNHKNSVTLGRAPLLSSLQRAAIL
                     TSEKFKGVRVNLEPGALRIASSNAEQEEAKEELEIDYAGDAIEIGFNVTYLMDALNNI
                     DQDMVKLELQDGNSSALITVPEQPGFKYVVMPMRI"
     gene            2507..5041
                     /gene="gyrB"
                     /locus_tag="VF453_18290"
     CDS             2507..5041
                     /gene="gyrB"
                     /locus_tag="VF453_18290"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX7689661.1"
                     /translation="MTEPTPHDDLPQDDLPAATPPADAAAYGEGSIQILEGLEAVRKR
                     PGMYIGDTSDGTGLHHLVFEVVDNSIDEALAGYCDDILVTIHTDNSISVIDNGRGIPT
                     GVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSTWLRLTV
                     RRDGKQHFIEFRKGVPQDRVLELRDGFEVSPMKITGDTERRGTEVHFLPDTEIFTNVD
                     FHYDILAKRLRELSFLNNGVKIRLVDERNAKEDNFAFAGGVKGFVEFINKGKTTLHPN
                     IFHAIGDKNSDQGTNIGVEVAMQWNDSFNESVLCFTNNIPQRDGGTHLTGLRAAMTRV
                     INKYIEDNELAKKAKVEIAGDDMREGLACVVSVKVPEPKFSSQTKDKLVSSEVRGPVE
                     DIVSRLLNDWLLENPSDAKIICGKIVDAARAREAARKAREMTRRKGVLDGMGLPGKLA
                     DCQEKDPAMCEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLSSEA
                     ILTLITALGTGIGKGTGGDDFNPEKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPE
                     LVERGHIYIAQPPLYKVKQGKHEQYLKDGSELDAFLLKVALDGAAIEPGEGRPAISGP
                     TLEELARKYVLASNVMDRLGNWMDVEALRAIADGLTLNLDDAAQAEASAVALAGALHD
                     AQVTALFDERTDKHILRIERQHHGNTKASTITQDFVHGADYEALAAAGSTFKGLLSGD
                     AVVRRGEGERQKDAKVADFRAAMAWLMAQAEGAVGRQRYKGLGEMNPEQLWETTMDPT
                     VRRLLKVEIEDAIEVDHVFTMLMGDEVEPRRNFIELNALRAANIDV"
BASE COUNT         1037 a         1877 c         1721 g          754 t
ORIGIN      
        1 cgtggtcgac gaggcgcctc ccggcgcgcg cccggcagcg cggccgatgc acgccgcgcc
       61 ggcgctcaac acgcaccgcc tgaaccccgc gctgaccttc gacaccctcg tggccggccg
      121 cgccaaccag atggcgcgca ccgccgccct gcacgtggcc ggtgcgccgg gcgcgatgta
      181 caacccgctg ttcgtctacg gcggcgtcgg gctgggcaag acccacctcg tgcacgcggt
      241 cggcaaccag ctgctgcgcg acaagcccga cgcgcgcgtg ctgtacctgc acgccgagca
      301 gttcatctcc gacgtcgtca agaactacca gcgcaagacc ttcgacgagc tcaaggccaa
      361 gtaccactcg ctcgacctgc tgctgatcga cgacgtccag ttcttcgccg gcaaggagcg
      421 cacgcaggag gaattcttca acgcgttcga ggcgctgctg gccaagcgcg cgcacatcat
      481 catgacgagc gacacctatc ccaagggtct cgccgacatc gacgaacgcc tgacgagccg
      541 cttcgactcc ggcctgaccg tcgccatcga gccgcccgag ctcgagatgc gcgtggccat
      601 cctgatccgc aaggccgagg ccgaaggcac gcagatgccc gaggaggtcg ccttcttcat
      661 cgcgaagaac gtgcgcgcca acgtgcgcga gctcgagggc gcgctgcgca aggtgctcgc
      721 gtactcgcgc ttctcgcaga aggagatcaa catcgcgctg gcgcgcgagg cgctcaagga
      781 cctgctgtcg atccagaacc gccagatctc cgtcgagaac atccagaaga cggtggccga
      841 cttctacaag atcaaggtgg ccgacatgta ctcgaagaag cgcccggcga gcatcgcccg
      901 cccgcgccag atcgcgatgt acctcgccaa ggagctgacg cagaagagcc tgcccgagat
      961 cggcgagctg ttcggcggcc gcgaccacac caccgtgctg catgccgtgc gcaagatcgg
     1021 cggcgagcgc cagaagaaca ccgagctgaa ccaggagctg cacgtcctgg agcagaccct
     1081 caagggatga atgacggccc ggctcgcttc ggagcggccg ggcgcgggcc gaccggcgac
     1141 ggcttcggtg gttcgaaggc cggccctgtt ttcaggcgaa aatacgcgct tcacgcgcgc
     1201 gcgcgtcggg ttcgggtttg cccttaaacg cgtcgcgcct cgcgcaaccc ggtcgacgac
     1261 aaagaggaag acatgattgt tctgaaggcg cctcaggaga agctgctcgg cgcattgcag
     1321 gcggtctcgg gcatcgtgga gcggcgccat acgctgccga tcctcgccaa cgtgctgatg
     1381 cgcaagaacg gcgagtccat cgagttcacc accagcgacc tcgagatcca ggtgcgcacc
     1441 agcgccgtgc tgggcggcga cgagggcagc ctctcgacga ccgtcgggtc gcgcaagatg
     1501 atcgacatcc tgaagtcgct gcccgccgac cagatcgtga ccctcacgtc ttcgggcaac
     1561 aagctgacgc tgcaggccgg caagagccgc ttcacgatgc agacgctgcc ggccgacgac
     1621 ttcccgctcg tcaacgaggc ggtcgacttc ggcccgagct tcggcgtgcc gcagaaggtg
     1681 ctcaagagcc tgatcgacca ggtgcacttc gcgatggcgg tgcacgacat ccgctactac
     1741 ctcaacggca tcctgttcgt cgccgagggc aagacgctca cgctggtggc caccgacggc
     1801 caccgcctcg gcctcgccca ggcgacgctg gagaccgaga tcccgaagca ggaggtcatc
     1861 ctcccgcgca agaccgtgct ggagcttgcg cgcctgctca acgcgacgac cgacgagccc
     1921 atcgagatgc gcttcgccgg caaccaggcg aagttcacct tctcggggct ggagttcgtc
     1981 accaagctgg tcgagggcaa gttccccgac tacaaccgcg tcatcccgaa gaaccacaag
     2041 aactcggtca cgctgggccg cgcgccgctg ctgtcgagcc tgcagcgcgc cgccatcctc
     2101 acgagcgaga agttcaaggg cgtgcgcgtg aacctcgagc cgggcgcgct gcgcatcgcc
     2161 tcgagcaacg ccgagcagga agaggccaag gaagaactcg agatcgacta cgcgggcgac
     2221 gcgatcgaga tcggcttcaa cgtgacctac ctgatggacg cgctcaacaa catcgaccag
     2281 gacatggtca agctcgaact gcaggacggc aactcgagcg cgctcatcac cgtgccggag
     2341 cagcccggct tcaagtacgt cgtgatgccg atgcgcatct gacgccgcgc ccgtcccgcg
     2401 ctgcacgccg ctccgcctcg cgcggagcgt gtttttttcg actggacaac gacaaggccc
     2461 cgcgcgcctc caggcgcggc aggcggccct caggaagcga atcgcgatga ccgaaccgac
     2521 cccccacgac gatctccccc aggacgacct gcccgcggcc acgccgcccg cggacgccgc
     2581 cgcgtacggc gagggctcga tccagatcct cgagggcctg gaggcggtgc gcaagcgccc
     2641 gggcatgtac atcggcgaca cctccgacgg caccggcctg caccatctcg tgttcgaggt
     2701 cgtcgacaac tcgatcgacg aggcgctcgc cggctactgc gacgacatcc tggtcacgat
     2761 ccacaccgac aactcgatct ccgtcatcga caacggccgc ggcatcccca ccggcgtgaa
     2821 gatggacgac aagcacgagc ccaagcgctc ggccgccgag atcgcgctga cggagctgca
     2881 cgccggcggc aagttcaacc agaacagcta caaggtctcg ggcggcctgc acggcgtggg
     2941 cgtctcctgc gtgaacgcgc tgtcgacctg gctgcgcctg accgtgcgcc gcgacggcaa
     3001 gcagcacttc atcgagttcc gcaagggcgt gccgcaggat cgcgtgctgg agctgcgcga
     3061 cggcttcgag gtcagcccga tgaagatcac cggcgacacg gagcgccgcg gcaccgaagt
     3121 ccacttcctg cccgacaccg agatcttcac caacgtcgac ttccactacg acatcctcgc
     3181 caagcgcctg cgcgagctct cgttcctgaa caacggcgtg aagatccgcc tcgtcgacga
     3241 gcgcaacgcg aaggaggaca acttcgcctt cgccggcggc gtcaagggct tcgtcgagtt
     3301 catcaacaag ggcaagacga cgctgcaccc gaacatcttc cacgcgatcg gcgacaagaa
     3361 cagcgaccag ggcaccaaca tcggcgtcga ggtcgcgatg cagtggaacg actcgttcaa
     3421 cgagtccgtg ctgtgcttca ccaacaacat cccgcagcgc gacggcggca cccacctgac
     3481 cggcctgcgc gcggcgatga cgcgcgtcat caacaagtac atcgaggaca acgagctcgc
     3541 gaagaaggcc aaggtggaga tcgccggcga cgacatgcgc gaaggcctcg cctgcgtcgt
     3601 cagcgtgaag gtgcccgagc cgaagttctc gagccagacc aaggacaagc tcgtctccag
     3661 cgaggtgcgc ggcccggtcg aggacatcgt cagccgcctg ctcaacgact ggctgctcga
     3721 gaacccgtcc gacgccaaga tcatctgcgg caagatcgtc gacgccgccc gcgcgcgcga
     3781 ggccgcccgc aaggcgcgcg agatgacgcg ccgcaagggc gtcctcgacg gcatgggcct
     3841 gccgggcaag ctcgccgact gccaggagaa ggacccggcg atgtgcgaga tctacatcgt
     3901 cgagggcgac tccgccggcg gctccgccaa gcagggccgc gaccgcaagt tccaggcgat
     3961 cctgcccctg cgcggcaaga tcctgaacgt ggagaaggcg cgctacgaga agctgctgtc
     4021 ctccgaggcc atcctgacgc tgatcaccgc gctgggcacc ggcatcggca agggcacggg
     4081 cggcgacgac ttcaaccccg agaagctgcg ctaccaccgc atcatcatca tgacggacgc
     4141 ggacgtcgac ggcgcccaca tccgcacgct gctgctcacg ttcttctacc gccagatgcc
     4201 cgagctcgtc gagcgcggtc acatctacat cgcgcagccg ccgctgtaca aggtcaagca
     4261 gggcaagcac gagcagtacc tgaaggacgg cagcgagctc gacgccttcc tgctgaaggt
     4321 ggcgctggac ggcgccgcca tcgagccggg cgaaggccgc cccgcgatct ccgggccgac
     4381 gctcgaggag ctcgcgcgca agtacgtgct cgcgagcaac gtgatggacc ggctcggcaa
     4441 ctggatggac gtcgaggcgc tgcgcgccat cgccgacggc ctgacgctga acctcgacga
     4501 cgccgcccag gccgaggcga gcgcggtggc gctggccggc gcgctgcacg acgcccaggt
     4561 caccgcgctg ttcgacgagc gcaccgacaa gcacatcctg cgcatcgagc gccagcacca
     4621 cggcaacacc aaggccagca ccatcacgca ggacttcgtc cacggcgccg actacgaggc
     4681 gctcgccgcc gccggcagca ccttcaaggg cctcctgagc ggcgacgccg tcgtccgccg
     4741 cggcgagggc gaacgccaga aggacgccaa ggtcgccgac ttccgcgccg ccatggcctg
     4801 gctcatggcc caggccgaag gcgccgtcgg ccgccagcgc tacaagggcc tcggcgagat
     4861 gaaccccgag cagctctggg aaacgaccat ggacccgacc gtccgccgcc tcctgaaggt
     4921 cgagatcgag gacgccatcg aggtcgacca tgtcttcacg atgctgatgg gcgacgaggt
     4981 ggagccgcgg aggaacttca tcgagctgaa tgcgttgagg gcggcgaata tcgacgtttg
     5041 atgttgtcgg gtgacacaga aagtgaaatc tctgccaagt ttgtgaaaag gtactgacag
     5101 cgttagtgac aggatctgcg cttggcgcgg acggcaaaac gcctggcgat gccaggcgtt
     5161 tgcattggga gtgccattac ctatccgctg gcggtcgtct cgatcggctg cgcccacggc
     5221 tcattctcga ggtgtctatt gatcgttcgc tagggatggt ctgcaaaacg tgacacggag
     5281 cgctgcgcag ccctgttgat gccacacgta tcgtcaggtc gcatgtctgt cggcctcgat
     5341 caggcggcag cgcaggcgct tggccctgtc gcagggcgca tttcaggct
//
LOCUS       DASVLN010000772         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_255205, whole genome shotgun sequence.
ACCESSION   DASVLN010000772 DASVLN010000000
VERSION     DASVLN010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255205"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF453_18295"
     CDS             complement(<1..197)
                     /locus_tag="VF453_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689662.1"
                     /translation="MADAAERIAICGLGLASPLGDEPGAAWARLVAGEGAVARLSFDG
                     LPDIAAATVPGDVSQGLTKLQ"
     gene            complement(205..450)
                     /locus_tag="VF453_18300"
     CDS             complement(205..450)
                     /locus_tag="VF453_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7689663.1"
                     /translation="MNTVDALKTLIHDKFGVELDAIDPDAPFASYQLDSLTVAELMFE
                     IDDAFHVQVPDTAANTVTTLRQLATLLDSLVEQRKAA"
     gene            complement(447..1358)
                     /locus_tag="VF453_18305"
     CDS             complement(447..1358)
                     /locus_tag="VF453_18305"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7689664.1"
                     /translation="MHSDPARTDRPTRLVLFHGLASSPKEFGFLAHPLRRQGVKLLAP
                     EIAGYSHGRLAPRPRWRDWVDAADDALATLGTDEPFVLGGLCTGSFLALALAARRPLP
                     GLRGVAMMSPSFACDGWALPWWYGLRRLAYALGLAGRFSMAERPPYGLKNERMRQWIR
                     QQLEAQASEPDAGGGVTPIGPARIGLPFVRESERLARHGRLLLARMALPLLVQHARED
                     EICRLETVERALRAAPLRYVSLQVLENSYHMITADNDRHLVADQLAAYMHSLAATDST
                     ARSGCEPDAASRSPATSSHGAPVAIAP"
BASE COUNT          218 a          592 c          678 g          219 t
ORIGIN      
        1 tgctgcagct tggtcagccc ctgcgagacg tcgccgggca ccgtcgcggc ggcgatgtcg
       61 ggcaggccgt cgaaggacag gcgcgcgacc gcgccctcgc cggcgacgag gcgcgcccag
      121 gcggcgccgg gctcgtcgcc cagcgggctc gcgaggccca gtccgcagat cgcgattcgt
      181 tcggcggcgt cggccatcgc ggcgtcaggc ggccttgcgc tgttcgacga ggctgtcgag
      241 cagggtcgcg agctggcgca gcgtcgtcac cgtgttggcg gccgtgtcgg gcacctggac
      301 gtggaaggcg tcgtcgatct cgaacatcag ctccgccacg gtcagcgagt cgagctggta
      361 gctggcgaac ggcgcgtcgg gatcgatggc gtccagttcc acgccgaact tgtcgtggat
      421 gagggtcttg agggcgtcga cggtgttcat ggggcgatcg ccacgggggc gccgtgggag
      481 gacgtggccg gggagcgcga cgcggcgtcg ggctcgcagc cggagcgggc ggtggagtcg
      541 gtggcggcca ggctgtgcat gtaggcggcg agctggtcgg cgacgaggtg ccggtcgttg
      601 tcggccgtga tcatgtgata gctgttttcc agcacctgca ggctgacgta gcgcaaaggc
      661 gccgcgcgca gggcgcgctc gaccgtctcg aggcggcaga tctcgtcctc gcgcgcatgc
      721 tgcaccagca gcggcaaggc catgcgggca agcagcaggc ggccgtgccg cgcgagccgc
      781 tcgctctcgc gcacgaacgg cagcccgatg cgcgcggggc cgatgggcgt gacgccgccg
      841 ccggcatccg gctcactggc ctgcgcctcg agctgctggc ggatccactg gcgcatgcgc
      901 tcgttcttga ggccgtaggg cgggcgctcg gccatcgaga agcgaccggc caggccgagt
      961 gcataggcga ggcggcgcag gccgtaccac cacggcagcg cccagccgtc gcaggcgaag
     1021 ctgggcgaca tcatcgccac gccgcgcagg cccggcagcg gccggcgcgc cgccagcgcg
     1081 agcgcgagga acgaccccgt gcacaggccg ccgagcacga agggctcgtc ggtgcccagc
     1141 gtcgcgagcg cgtcgtcggc cgcgtccacc cagtcgcgcc agcgcgggcg cggcgcgagt
     1201 cggccgtggc tgtagccggc gatctcgggc gcgaggagct tcacgccctg gcggcgcagc
     1261 gggtgggcga ggaagccgaa ttccttgggc gagctcgcga ggccgtggaa gaggaccagc
     1321 cgcgtcgggc ggtcggtccg agcggggtcg gaatgcatcg atggggggag acctgaaacg
     1381 gtcgcggcgg tcgagccggg cgggccgcgc cgggaggcaa cggacgggag cagggcgacg
     1441 ccgcggcgac ggttccaatt gtccccgcgc ggccggtcgc gagccccggc gctcgcttgc
     1501 ttcaaactgt cgacaggctg tcgtgcggga cccgggcagt cccggaagcg gggccggcca
     1561 gggccgatac actgcccggg ccggcgatcc cggctcccct ggcgcgccgc gcgacccgcg
     1621 cggtgccgct gcccctccgc tgtccacccc cgcatgccct ccgccttcca tcccttcgcg
     1681 accctgctgc tggccgccgc gctgccg
//
LOCUS       DASVLN010000773         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_525577, whole genome shotgun sequence.
ACCESSION   DASVLN010000773 DASVLN010000000
VERSION     DASVLN010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525577"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VF453_18310"
     CDS             <1..120
                     /locus_tag="VF453_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitropropane dioxygenase"
                     /protein_id="HEX7689665.1"
                     /translation="AAAEQAGRGDFSPLWAGQNASGCRAVPAAAIVQALAEGF"
     gene            complement(133..1476)
                     /locus_tag="VF453_18315"
     CDS             complement(133..1476)
                     /locus_tag="VF453_18315"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX7689666.1"
                     /translation="MTHFPADPSSQRRSRSARLPVALAAALALGLAGCGGGDDGSSAS
                     ITLGGTVSSLTTDGLVLADNGATVSVPANATTFTFGSVSGSYDVTILSQPAGQTCTVA
                     NGSGSTSGSSVDNVAVTCRAYAVFVANQGSGNIGQFLVASDGTLTAQAAGALATPFMP
                     DSLVVSADGQHAWVAYADDHSLSNLTIGANGALALASGTPEAISTQDALGLGADGKSL
                     YAAEYGATQVSQFAIGTGGLLGSAPVASVAAGVNPSAVAATADGAHLYVANASADSVS
                     GYALDANGKPTALSPAITSTTSYGSSPRGLAVNPNGGYLYVTLANSAKVVAYGVDAGS
                     GALTFRSSQATGTTPRGIAVTPSGAYAYVANNGSNTISQYTLAGGSLTPLSTPTVAAG
                     SHPVAIAISPDGAFAYAANSGDNSVSVYAIGGNGQLTPVATTPSGGTQPASIAVR"
BASE COUNT          223 a          588 c          730 g          234 t
ORIGIN      
        1 gcggccgccg agcaggcggg ccgcggcgac ttctcgccgc tgtgggccgg ccagaacgcg
       61 agcggttgcc gcgcggtgcc cgcggccgcg atcgtccagg cgctggccga gggcttctga
      121 ccgcgccgtc gctcagcgca ccgcgatcga ggcgggctgc gtgccgccgc tcggcgtcgt
      181 ggccaccggc gtgagctggc cgttcccgcc gatcgcgtag accgagacgc tgttgtcgcc
      241 cgagttggcg gcgtaggcga acgcgccgtc cggactgatg gcgatggcca ccgggtgcga
      301 gcccgccgcg acggtgggcg tggacagcgg cgtcaggctg ccgccggcca gcgtgtactg
      361 cgagatcgtg ttcgagccgt tgttggcgac gtaggcgtag gcgcccgatg gggtcaccgc
      421 gatgccgcgc ggcgtcgtgc cggtcgcctg gctgctgcgg aaggtgagcg cgccggagcc
      481 cgcatcgacg ccgtaggcga cgaccttggc gctgttggcg agcgtcacgt acaggtagcc
      541 gccgttcggg ttgaccgcga ggccgcgagg cgacgagccg taggacgtgg tgctggtgat
      601 cgcgggcgag agcgcggtcg gcttgccgtt ggcgtcgagc gcgtagccgg agacgctgtc
      661 cgcgctcgcg ttggcgacgt acaggtgcgc gccgtcggcc gtcgcggcca cggcgctcgg
      721 gttgaccccc gcggcgaccg aggcgaccgg cgccgacccc agcaggccgc cggtaccgat
      781 ggcgaactgc gagacctgcg tcgcgccgta ctcggccgcg tacagcgact tgccgtccgc
      841 gcccaggccc agcgcgtcct gcgtgctgat cgcctcgggc gtgcccgagg ccagcgccag
      901 cgcgccgttc gccccgatgg tgaggttgga cagcgagtgg tcgtccgcgt acgcgaccca
      961 ggcatgctgg ccgtcggcgc tgacgacgag cgagtcgggc atgaacggcg tcgccagggc
     1021 gccggccgcc tgcgccgtca gcgtgccgtc ggacgcgacc aggaactggc cgatgttgcc
     1081 ggagccctgg ttggcgacga acacggcgta ggcacggcag gtcaccgcga cgttgtcgac
     1141 cgagctgccg ctggtcgagc cgctgccgtt ggcgaccgtg caggtctggc cggcgggctg
     1201 cgacaggatc gtcacgtcgt agctgcccga gacgctgccg aaggtgaagg tggtcgcgtt
     1261 ggccggcacg ctgacggtgg cgccgttgtc cgcgagcacg aggccgtcgg tcgtgagcga
     1321 ggagacggtg ccgccgagcg tgatgctggc actgctgccg tcgtcgccgc cgccgcagcc
     1381 ggcgaggccg agcgcgagcg cggcggcgag cgcgacgggc aggcgcgcgg agcgcgaccg
     1441 gcgctgggac gagggatcgg ccgggaaatg ggtcatgcgg ggctcccagg ggatttggag
     1501 ttgtgtcgga tcaggactgt aggcgctgcg cggccgcgcg cccggtggaa ttgagccgga
     1561 aaagacgggg atttacgcgc cggcaaaagt gataccgggc gcgtccggcc cggcggcgtc
     1621 aggcgggggg ctcgaagacg aacgagccgg gcagcagctc gcccgcggtg cgcacgacga
     1681 ggcggccgtc ctcggcgtcg ctggcgaccg gggcggccgc gtcggcgaac tcgaggagga
     1741 cctggcggca cgcgccgcag ggcgtgatcg ggtgc
//
LOCUS       DASVLN010000774         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_525591, whole genome shotgun sequence.
ACCESSION   DASVLN010000774 DASVLN010000000
VERSION     DASVLN010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525591"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /gene="nadB"
                     /locus_tag="VF453_18320"
     CDS             <1..1596
                     /gene="nadB"
                     /locus_tag="VF453_18320"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX7689667.1"
                     /translation="AGLAGLTVALHLARHGRRVIVLAKRGLSEGATSWAQGGIVGVLG
                     SDDSIDSHVRDTQDAGGGIVDEHAARFIAQNSARAVEWLVDNGVPFSPDPDGPLGLHL
                     TREGGHAVRRIAHAADATGKAIHESLLAQARAHELISLRERWIAVDLITSRHLQRKET
                     PRTYGVYALDIDKRTVETIAASAVVLATGGVGKVYRYTTNPDTSTGDGIAMAWRAGCR
                     VANMEFIQFHPTCLYHPQERSFLITEALRGEGAVLRLPVQGTPDEPWGPRFMQEHDER
                     GELAPRDIVARAIDFEMKKHGVDHVWLDATHLSEAFLKEHFPTIHARCLSLGIDIARQ
                     PIPVVPAAHYTCGGMVTDLSGRADLPGLYGVGETSYTGLHGANRLASNSLLECVVIGK
                     TCADAILADVLPDAAGLPEWDESKVDDADEQVVIAHNWDELRLLMWNYVGIVRTTKRL
                     ERALHRIELLKAEIDEYYAHFRVTRDLLELRNLVVCAELIVRSGLNRHESRGLHFSRD
                     FPRTLPVSFPTILTRPATSRPAA"
     gene            complement(1675..>2020)
                     /locus_tag="VF453_18325"
     CDS             complement(1675..>2020)
                     /locus_tag="VF453_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7689668.1"
                     /translation="GATVTAMLMVAAAFALTRLAVPGTTAGLAILVVAGIVLDAGVTT
                     NLVVGQRAIFALGADVRSRLNGLFIAAFFAGGAIGSALGGYVFAEGGWPGVAWVGLAL
                     PLAALAWFATER"
BASE COUNT          308 a          763 c          701 g          248 t
ORIGIN      
        1 gccggcctcg ccggcctgac ggtggcgctg cacctcgcgc gccacggccg gcgcgtcatc
       61 gtgctggcca agcgcggcct gagcgaaggg gcgacctcgt gggcgcaggg cggcatcgtc
      121 ggcgtgctgg gctcggacga ctcgatcgac tcccacgtgc gcgacaccca ggacgccggc
      181 ggcggcatcg tcgacgagca cgccgcccgc ttcatcgccc agaacagcgc gcgcgccgtc
      241 gaatggctgg tggacaacgg cgtgccgttc tcgccggatc ccgacggccc gctggggctg
      301 cacctgacgc gcgagggcgg ccacgcggtg cgccgcatcg cgcacgcggc cgacgccacg
      361 ggcaaggcga tccacgagtc gctgcttgcg caggcccggg cgcacgagct catctcgctg
      421 cgcgagcgct ggatcgccgt cgacctcatc acctcgcgcc acctgcagcg caaggagacg
      481 ccgcgcacct acggcgtcta cgcgctggac atcgacaagc gcaccgtcga gaccatcgcc
      541 gcctcggccg tggtgctcgc caccggcggc gtcggcaagg tctaccgcta cacgacgaat
      601 ccggacacct ccaccggcga cggcatcgcg atggcctggc gcgcgggctg ccgcgtggcc
      661 aacatggaat tcatccagtt ccacccgacc tgcctgtacc acccgcagga gcgcagcttc
      721 ctcatcaccg aggcgctgcg cggcgagggc gccgtgctgc gcctgccggt gcagggcacg
      781 ccggacgagc cctgggggcc gcgcttcatg caggagcacg acgagcgcgg cgagctcgcg
      841 ccgcgcgaca tcgtggcgcg cgcgatcgac ttcgagatga agaagcacgg cgtcgaccac
      901 gtctggctcg acgcgacgca cctatcggag gcgttcctca aggaacactt cccgaccatc
      961 cacgcgcgct gcctgtcgct gggcatcgac atcgcgcgcc agccgatccc cgtcgtcccg
     1021 gccgcgcact acacctgcgg cggcatggtg acggaccttt cgggccgtgc cgacctgccg
     1081 ggcctgtacg gcgtcggcga gacctcgtac accggcctgc atggcgccaa ccggctcgcg
     1141 agcaactcgc tgctcgaatg cgtcgtgatc ggcaagacct gcgcggacgc catcctcgcc
     1201 gacgtgctgc cggacgccgc gggcctgccc gagtgggacg agagcaaggt cgacgacgcc
     1261 gacgagcagg tcgtcatcgc ccacaactgg gacgagctgc gcctgctgat gtggaactac
     1321 gtggggatcg tgcgcacgac caagcggctc gagcgcgcac tgcaccgcat cgagctgctc
     1381 aaggccgaga tcgacgagta ctacgcccac ttccgcgtca cgcgcgacct gctcgagctg
     1441 cgcaacctgg tcgtctgcgc ggagctgatc gtgcgctccg gcctgaaccg ccacgagagc
     1501 cgcggcctgc acttcagccg cgacttcccg cgcacgctgc cggtgagctt cccgacgatc
     1561 ctcacgcggc cggcgacctc gcggcccgcg gcctgagcga gcgtccggga agccgtcgtc
     1621 cgcccggcgc gcggcgggct cgagaggccg tgagcctcga gggcacggat atctctatcg
     1681 ctccgtcgcg aaccacgcca gcgccgccag cggcagcgcc agtccgaccc acgcgacccc
     1741 gggccagccg ccttcggcga acacatagcc gcccagggcc gagccgatcg cgccgcccgc
     1801 gaagaaggcg gcgatgaaca ggccgttgag gcggctgcgg acgtccgcgc cgagcgcgaa
     1861 gatcgcgcgc tggccgacca cgaggttggt cgtgacgccg gcgtccagca cgatgccggc
     1921 gacgaccagg atcgcgaggc cggccgtcgt gcccggcacg gccaggcgcg tgagcgcgaa
     1981 ggcggcggcg accatcagca tcgccgtgac ggtggcgccg
//
LOCUS       DASVLN010000775         5224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_416058, whole genome shotgun sequence.
ACCESSION   DASVLN010000775 DASVLN010000000
VERSION     DASVLN010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416058"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..83
                     /locus_tag="VF453_18330"
     CDS             <1..83
                     /locus_tag="VF453_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7689669.1"
                     /translation="RPPEPYKGKGIRYADERVVLKETKKK"
     gene            95..451
                     /gene="rplR"
                     /locus_tag="VF453_18335"
     CDS             95..451
                     /gene="rplR"
                     /locus_tag="VF453_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX7689670.1"
                     /translation="MLNKKEQRLRRSRQTRARIAKQDIARLTVFRSNLHIYASVISND
                     GARVVASASTAEKEVREQLKNGGNAAAAAVVGKRIAEKAKAAGVEKVAFDRAGFAYHG
                     RVKALAEAAREAGLQF"
     gene            474..992
                     /gene="rpsE"
                     /locus_tag="VF453_18340"
     CDS             474..992
                     /gene="rpsE"
                     /locus_tag="VF453_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX7689671.1"
                     /translation="MAKFQPRVQDEGRDDGLREKMIAVNRVTKVVKGGRILGFAALTV
                     VGDGDGRIGMGKGKAREVPVAVQKAMEQARRNMVKVSLKNGTIHHNVRGEHGAANVLM
                     APAPAGTGIIAGGPMRAVFEVMGITDIVAKSLGSSNPYNMVRATLDALNNCTTPADIA
                     AKRGKTVEEILG"
     gene            1007..1189
                     /gene="rpmD"
                     /locus_tag="VF453_18345"
     CDS             1007..1189
                     /gene="rpmD"
                     /locus_tag="VF453_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX7689672.1"
                     /translation="MAEKKTLRVKLVRSTIGTKQSHRDTVRGLGLRRLNSESVLEDTP
                     AVRGMIDKVAYLVLVL"
     gene            1216..1650
                     /gene="rplO"
                     /locus_tag="VF453_18350"
     CDS             1216..1650
                     /gene="rplO"
                     /locus_tag="VF453_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX7689673.1"
                     /translation="MQLNTIKPASGAKHAKRRVGRGIGSGLGKTAGRGHNGQHSRAGG
                     YHKVGFEGGQMPLQRRLPKRGFKSLALQYNGEVTLSDLQAMAIDEIDVPALKAAGLVG
                     QLIRNVKVIKSGELSRKVTLKGVAATAGAKAAIEAAGGSLAD"
     gene            1694..3001
                     /gene="secY"
                     /locus_tag="VF453_18355"
     CDS             1694..3001
                     /gene="secY"
                     /locus_tag="VF453_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX7689674.1"
                     /translation="MATSQNQLAKSGNFGDLRRRVMFLLLALVVYRIGGHIPVPGVNP
                     DELAKMIQSQQGGLMGMFNMFSGGSLSRASVFALGITPYISSSIILQIMGYVVPSLEA
                     LKKEGESGRRKITQYTRYGTVGLSLVQSYGIAAWLEKSPGLVLNPGLGFVIPAVVSLV
                     AGTMFMMWLGEQITERGLGNGISILIFGGIAAGLPRAITGLLELVSSGSMNPVVALFI
                     LVIVAAVTFAVVFVERGQRKILVNYAKRQVGNKVYGGQSSHLPLKLNMSGVIPPIFAS
                     SIILLPATLVNSFATSEGLRWLKNISSALSPGQPIYVLLYAAMIVFFCFFYTGLVFNS
                     NETADNLKKSGALIPGIRPGQQTAKYIDKILARLTLAGSIYITLVCLLPEFLVLKYNV
                     PFYFGGTSLLIIVVVTMDFWAQVQSYVMSQQYESLLTKAKFKA"
     gene            3113..3226
                     /gene="rpmJ"
                     /locus_tag="VF453_18360"
     CDS             3113..3226
                     /gene="rpmJ"
                     /locus_tag="VF453_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX7689675.1"
                     /translation="MKVSASVKKMCRNCKVIRRKGVVRVICTDPRHKQRQG"
     gene            3265..3630
                     /gene="rpsM"
                     /locus_tag="VF453_18365"
     CDS             3265..3630
                     /gene="rpsM"
                     /locus_tag="VF453_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX7689676.1"
                     /translation="MARIAGINIPPHKHTEIGLTSIYGIGRTTAQQICDRTGIAYSKK
                     IKDLSDSDLEKIRDEIAKITIEGDLRRETSINIKRLMDLGCYRGMRHRRGLPVRGQRT
                     RTNARTRKGPKKGAAALKK"
     gene            3672..4076
                     /gene="rpsK"
                     /locus_tag="VF453_18370"
     CDS             3672..4076
                     /gene="rpsK"
                     /locus_tag="VF453_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX7689677.1"
                     /translation="MAKAPNNSAAQRVRKKVRKNVADGVAHVHASFNNTIITITDRQG
                     GALSWASSGGQGFKGSRKSTPFAAQVAAENAGRAAQEQGIKNLDVKIKGPGPGRESSV
                     RALASLGIRITSISDVTPVPHNGCRPQKRRRI"
     gene            4363..4986
                     /gene="rpsD"
                     /locus_tag="VF453_18375"
     CDS             4363..4986
                     /gene="rpsD"
                     /locus_tag="VF453_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7689678.1"
                     /translation="MARYLGPKGKLSRREGTDLYLKSARRAIGDKVKFDSKPGQHGRT
                     SGSRTSDFGVQLREKQKVKRMYGVLEKQFRKYFAEAERRKGNTGANLLFLLESRLDNV
                     VYRMGFGSTRAEARQLVSHKAITVNGTVVNISSYLVKVGDVIAVREKAKKQLRITEAL
                     KLAESIGLPAWVSVDATKLEGTFKKVPDRDEFGAEINESMIVELYSR"
BASE COUNT         1014 a         1704 c         1690 g          816 t
ORIGIN      
        1 tccgtccgcc cgagccgtac aagggcaagg gcatccgcta cgccgatgag cgcgtggtgc
       61 tgaaagagac gaagaagaag taaggagcgc caacatgctg aacaagaagg aacagcgcct
      121 gcgccgttcg cgccagacgc gtgcccgcat cgcgaagcag gatatcgcgc gcctgacggt
      181 cttccgctcg aacctgcaca tctacgcgag cgtgatctcc aacgacggcg cccgcgtcgt
      241 cgccagcgcc tcgacggccg agaaggaagt ccgcgagcag ctcaagaacg gtggcaatgc
      301 cgccgcggct gccgtggtcg gcaagcgcat cgccgagaag gcgaaggccg ccggcgtcga
      361 gaaggtggcg ttcgaccgtg cgggcttcgc ctatcacggc cgtgtgaagg ccctggcgga
      421 agcggctcgc gaagccggcc tccagttctg acgcgcaacg aaggaatctg caaatggcaa
      481 agtttcaacc tcgcgtgcag gacgaaggtc gcgacgacgg tctgcgcgag aagatgatcg
      541 cggtcaaccg cgtcaccaag gtcgtgaagg gtggccggat cctcggcttc gccgcgctga
      601 ccgtggttgg cgacggcgat ggccgcatcg gcatgggcaa gggcaaggcg cgcgaagtgc
      661 cggtggccgt ccagaaggcg atggagcaag cccgtcgcaa catggtcaag gttagcctga
      721 agaacggcac gatccaccac aacgtccgcg gcgagcacgg cgcggccaac gtgctgatgg
      781 ccccggcccc ggccggtacc ggcatcatcg ccggcggccc gatgcgcgcg gtcttcgaag
      841 tgatgggcat caccgacatc gtcgccaaga gcctcggctc gagcaacccg tacaacatgg
      901 tgcgcgcgac gctggacgcc ctgaacaact gcacgacccc ggccgacatc gccgccaagc
      961 gcggcaagac ggtcgaagag atcctcggct gatcgggagt ttcgaaatgg ctgaaaagaa
     1021 gacgctgcgc gtcaagctcg tgcgcagcac gatcggcacc aagcaatcgc accgcgacac
     1081 ggtgcgcggc ctgggcctgc gccgcctgaa cagcgagtcc gtcctcgaag acacgcccgc
     1141 ggttcgcggc atgatcgaca aggtcgccta tctcgttctg gtgctgtaag taccggacga
     1201 caaggaatcg acaccatgca actcaacacc atcaagcccg ccagcggcgc gaagcacgcc
     1261 aagcgtcgcg tcggccgcgg catcggttcg ggcctgggca agacggcggg ccgcggccac
     1321 aacggccagc actcgcgcgc cggcggctac cacaaggtcg gcttcgaagg cggccagatg
     1381 ccgctgcagc gtcgcctgcc caagcgcggc ttcaagtcgc tcgcccttca gtacaacggc
     1441 gaggtcacgc tctcggacct gcaggcgatg gcgatcgacg agatcgacgt ccccgcgctg
     1501 aaggctgccg gcctggtcgg ccagctgatc cgcaacgtca aggtcatcaa gtccggcgag
     1561 ctcagccgca aggtcacgct caagggcgtg gcggcgacgg cgggtgcgaa ggcggcgatc
     1621 gaggcggccg gcggttcgct ggcggactga acgcgcgctt cggtacttcg aacgcatctc
     1681 acgcgaccca cacgtggcaa cttcccagaa tcaactcgcc aagtcgggca acttcggtga
     1741 cctccgtcgc cgggtgatgt tcctgctgct ggcgctggtg gtctatcgga tcggcgggca
     1801 cattcccgtg cccggcgtca atccggacga gctcgccaag atgatccaga gccagcaggg
     1861 cggcctgatg ggcatgttca acatgttctc gggcggctcg ctgtcgcgcg cctccgtgtt
     1921 cgcgctgggc atcacgccgt acatctcgtc ctcgatcatc ctgcagatca tgggctacgt
     1981 cgtgccgtcg ctggaggcgc tgaagaagga aggcgagtcg ggtcgtcgca agatcacgca
     2041 gtacacgcgc tacggcacgg tcggcctgtc gctggtgcag tcctacggca tcgccgcctg
     2101 gctcgagaag tctccgggcc tggtgctcaa tcccggcctg ggcttcgtca tcccggccgt
     2161 ggtgagcctg gtcgccggca cgatgttcat gatgtggctc ggcgagcaga tcaccgagcg
     2221 cggcctgggc aacggcatct cgatcctcat cttcggcggc atcgccgcgg gcctgccccg
     2281 cgcgatcacg ggcctgctgg aactggtgag ctcgggctcg atgaacccgg tcgtcgcgct
     2341 gttcatcctg gtgatcgtgg cggcggtgac gttcgcggtc gtcttcgtgg agcgcggcca
     2401 gcgcaagatc ctggtcaact acgcgaagcg ccaggtgggc aacaaggtgt acggcggcca
     2461 gtcgtcgcac ctgccgctga agctgaacat gtcgggcgtg atcccgccga tcttcgcgtc
     2521 gtcgatcatc ctgctgccgg ccacgctcgt gaactcgttc gcgaccagcg aaggcctgcg
     2581 ctggctgaag aacatctcgt ccgcgctgtc gccgggccag ccgatctatg tgctgctcta
     2641 cgccgcgatg atcgtgttct tctgcttctt ctacaccggc ctcgtgttca acagcaacga
     2701 gaccgcggac aacctgaaga agagcggcgc gctgatcccg ggcatccgtc cgggccagca
     2761 gacggcgaag tacatcgaca agatcctggc gcgcctgacc ctggccggct cgatctacat
     2821 cacgctggtg tgcctgctgc ccgaattcct ggtgctgaag tacaacgtgc cgttctactt
     2881 cggcgggacg tcgctgctga tcatcgtcgt cgtcacgatg gacttctggg cccaggtgca
     2941 gagctacgtg atgagccagc agtacgaatc gttgctcacg aaggccaagt tcaaggcttg
     3001 agtcattgaa tggaaggcgg gcgcgcccag atgggcgggt cgccgacaaa gtttgagtcg
     3061 cgctccgctt cgcccaagcg agcgaagcga gtgttgaaag aaggagcaga ccatgaaagt
     3121 ctcggcatct gtcaagaaaa tgtgccgcaa ttgcaaggtg atccgccgga agggcgtcgt
     3181 ccgtgtgatt tgtaccgacc cgcgccacaa gcagcgccag ggctaaccgt cggctggcga
     3241 tagaagcatt ccaaggacac gcaaatggca cgcattgcag gcatcaacat cccgccgcac
     3301 aagcacaccg agatcggcct gacgtcgatc tacggtatcg gccgcacgac cgcccagcag
     3361 atctgcgatc gcacgggcat cgcgtactcg aagaagatca aggacctgtc ggacagcgac
     3421 ctcgagaaga tccgcgacga gatcgcgaag atcacgatcg aaggcgacct gcgccgcgag
     3481 acgtccatca acatcaagcg cctgatggac ctgggctgct atcgtggcat gcgtcatcgc
     3541 cgcggcctgc cggtccgcgg ccagcgcacc cgcaccaacg cccgtacccg caagggtccg
     3601 aagaagggtg ccgcggcgct gaagaagtaa gcggttgagt tcgctcgacg actgaaacaa
     3661 agaaggtcaa catggctaaa gcaccgaaca actccgctgc tcaacgcgtc cgcaagaagg
     3721 tccgcaagaa cgtggccgac ggcgtcgcgc acgtgcacgc gtcgttcaac aacaccatca
     3781 tcacgatcac ggaccgccag ggcggcgcgc tgtcgtgggc ctcgtcgggc ggtcagggct
     3841 tcaagggctc gcgcaagtcg accccgttcg ctgcccaggt cgccgccgag aacgccggcc
     3901 gtgccgcgca ggaacagggc atcaagaacc tcgacgtcaa gatcaagggc ccgggcccgg
     3961 gtcgcgagtc gtcggttcgc gcgctcgcgt ccctgggcat ccgcatcacg tcgatctccg
     4021 acgtgacgcc ggtcccgcac aacggctgcc gtccgcagaa gcgtcgtcgc atctgatgcc
     4081 ggcgtgcggc gcgggggcaa cccggccgtc gccgaaaaac agcactgtgg acgatggtgt
     4141 tcacagtgct gctacaattc cgggttttcc tgcagccggc aatggtttga acaccgttgc
     4201 cggcttgcat gtctcgaacg tcccgatctc ctcgccgagc cggggcggcc aacccaagac
     4261 caccgtctga gcgccagaac aatatccgcc agactcgcgc atcgcgaccg cccgcacctc
     4321 gcgggctgag catgcgtcag caatttttcc caaggaaaca acgtggcacg ttacctcggc
     4381 cccaagggca agctctcccg ccgtgaaggc accgacctgt acctgaagag cgcgcgtcgc
     4441 gccatcggcg acaaggtcaa gttcgactcg aagcccggcc agcacggccg tacctcgggt
     4501 tcgcgtacct cggacttcgg cgtgcagctg cgcgagaagc agaaggtcaa gcgcatgtac
     4561 ggcgtgctgg agaagcagtt ccgcaagtac ttcgcggagg ccgagcgccg caagggcaac
     4621 accggcgcca acctgctgtt cctgctcgaa tcgcgcctgg acaacgtggt ctaccgcatg
     4681 ggcttcggct cgacgcgtgc cgaagcgcgc cagctggtct cgcacaaggc catcacggtc
     4741 aacggcaccg tcgtgaacat ctcgtcgtac ctggtcaagg tcggcgacgt catcgcggtg
     4801 cgcgagaagg ccaagaagca gctgcgcatc accgaggcgc tgaagctcgc cgagtcgatc
     4861 gggctgccgg cgtgggtgtc ggtcgacgcc accaagctcg agggcacgtt caagaaggtg
     4921 ccggaccgcg acgagttcgg cgccgagatc aacgaatcga tgatcgtcga actgtactcg
     4981 cgctgatccg aattcgtggt cgccacgctc ggcctcgagc cgggcagcga ccaccgtccc
     5041 gcggcgaggc gccgggcttc gcggcaatgg acgtacaggg cttcccggcg tggtccatca
     5101 gccttatcgg tgtaacgagc cgagggtatt gagaggaaca ggacatacat gcaaaccaat
     5161 ctgctgaaac ccaaagccat caatgtcgag ccgctcggcg ggcatcgtgc caaggtcacc
     5221 ctcg
//
LOCUS       DASVLN010000776         6389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_94398, whole genome shotgun sequence.
ACCESSION   DASVLN010000776 DASVLN010000000
VERSION     DASVLN010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94398"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VF453_18380"
     CDS             complement(<1..473)
                     /locus_tag="VF453_18380"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEX7689679.1"
                     /translation="MKLDELAARAAGPAAPPGLRFIRAVDVAASPWKNGGGTTRELLR
                     VAAPGAAADDWTLRISLADIAADGPFSPFPGVTRWFSVIEGTGVHLAWTSEDGLHERR
                     VAVGPEYGPLRFDGAEAPDCRLLDGPTRDLNVMARDGAATAEVVPAAFDKPCGWAG"
     gene            complement(535..1356)
                     /gene="hutC"
                     /locus_tag="VF453_18385"
     CDS             complement(535..1356)
                     /gene="hutC"
                     /locus_tag="VF453_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HEX7689680.1"
                     /translation="MSSSPPSPRSRLRTAAAPAALGLRASPGDHAAGGLPSSKSPPQP
                     PYARVKQFLKDALAQGRWAPGQQMPSEAELVAQFGVSRMTVHRALRELQDAGFITRLQ
                     GVGTFAAQLNRVSSTLTINDIQEEIQSRGHRHDATVHLQRAEAAPAALAQRLGLATGA
                     PVFHTLIVHHEHGVPLQCEDRYVNPASAPDYLAQDFTQRTPTHYLLDVAPLWEAQVSI
                     EAALPTAQEARLLGIARTDPCLVVVRRTVSRGLPVTLARLVHPGSRYALESAFKP"
     gene            1516..3210
                     /gene="hutU"
                     /locus_tag="VF453_18390"
     CDS             1516..3210
                     /gene="hutU"
                     /locus_tag="VF453_18390"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX7689681.1"
                     /translation="MTDLSNAHALPRPVRAPRGTELACRNWLVEAAYRMIQNNLDPEV
                     AENPNALVVYGGIGKAARNWDCFEAILEALRKLGEDETLLVQSGKPVGVFRTHADAPR
                     VLLANSNLVPKWATWEHFHELDRRGLMMFGQMTAGSWIYIGSQGIVQGTYETFVEAGR
                     QHYGGSLAGRWILTAGLGGMGGAQPLAASFAGASSLTIECQQSRIDFRLKTRYVDEQA
                     ADLDDALARLARYTAEKRAVSIALLGNAAEVLPELVRRARAGGPRPDLVTDQTSAHDL
                     VNGYLPVGWSVAQWREAAADHARHAELRDAAAKGCAVHVQAMLDFQAMGVPTVDYGNN
                     IRQVAFDQGVANAFDFPGFVPAYVRPLFCQGKGPFRWVALSGDPEDIRRTDAKMKELF
                     PEDAHLHRWLDMAGERIAFQGLPARICWIGLGDRDRAGRAFNEMVRTGELKAPIVIGR
                     DHLDSGSVASPNRETESMKDGSDAVSDWPLLNALLNTAGGATWVSLHHGGGVGMGYSQ
                     HAGVVIVCDGTKEADARLARVLWNDPATGVMRHADAGYDDARAAAKRHGLNLPMIK"
     gene            3216..4766
                     /gene="hutH"
                     /locus_tag="VF453_18395"
     CDS             3216..4766
                     /gene="hutH"
                     /locus_tag="VF453_18395"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX7689682.1"
                     /translation="MTQTLLLKPGQLTLADLQALHAGGVRLALAPEARAAIAASAAVV
                     QAAALGDAPVYGVNTGFGKLANKRISQDELDALQRNLIRSHSVGVGEPLQPAVVRLML
                     AIKAASLARGHSGVREAVVDTLVDAYNAGLVPYVPSQGSVGASGDLAPLSHMTLALMG
                     EGEMLVDGRRVPALPELEKAGLAPLRLEAKEGLALINGTQTSTALALHALISFEPVLE
                     SALVIGALTVDAARGSDGPFDPRIHALRGQPGQIDVARYYRALLAGSEIRRSHLEGDD
                     RVQDPYCLRCQPQVVGACLDQLRHAALVLVREANAVTDNPLVFAEDGVLVSGGNFHAE
                     PVALACDAMAVAIAEVGAIAERRIAMLIDAGVSRLPPFLSADAGLNSGFMIAHVTAAS
                     VASENKSLAHPASVDSLPTSANQEDHVSMATFAARRLQAMIANVATILGIEWLASAQG
                     VEFLRPLRSSAALEQAHALLREQCAAMPTDRWLAPDIERATALVAGGSLSRVFRTLEG
                     LPALWTPA"
     gene            4927..6090
                     /locus_tag="VF453_18400"
     CDS             4927..6090
                     /locus_tag="VF453_18400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7689683.1"
                     /translation="MSKVPPETPGYEANGRPAARLRALLQRWMRSPAAGRDVLGEYRD
                     RIMLPMAAASVALLLPFAAWDFLSGKWLLGALAFAVCVPLALDAAALHRRRKPPVAYG
                     WILLPIAAAIAAALLARGVHGAMWCYPAILFCYFVLPGWQATAGALGLMAMTSALAWH
                     VLGAPTTVRFAVTSGLSVIILNIILGVVGELQKKLYEQATTDPLTGALNRRQLAQTLA
                     DAIEHSRRLEAPVSLLAFDLDHFKRVNDRHGHAAGDAVLQRVVGLVQERSRRIDRLFR
                     MGGEEFALLLPGTNVADAMRVASVLRERVAGADWFDDARITVSVGVAQLHRKHTVDSW
                     LKSADDALYRAKVDGRNCVRVATRSRTNAAGELVDTGFSRPSDLVPLVPAARD"
     gene            complement(6160..>6389)
                     /locus_tag="VF453_18405"
     CDS             complement(6160..>6389)
                     /locus_tag="VF453_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7689684.1"
                     /translation="LVVLSVPHFLFLRAALRETDLVAVLPERLVRATPGLRVVEPPVE
                     VPGYEMAMVWHERVHRDPAHRWLREVVAAAV"
BASE COUNT          827 a         2366 c         2359 g          837 t
ORIGIN      
        1 ccggcccagc cgcagggctt gtcgaacgcg gccgggacga cctcggccgt cgccgcgccg
       61 tcgcgggcca tgacgttgag gtcgcgcgtg gggccgtcga gcagccggca gtccggcgcc
      121 tcggcgccgt cgaatcgcag cgggccgtat tccgggccga ccgcgacgcg gcgctcgtgc
      181 aggccgtcct cgctggtcca cgcgaggtgc acgcccgtgc cctcgatcac ggagaaccag
      241 cgcgtgacgc cggggaaggg cgagaacggg ccgtcggcgg cgatgtcggc gaggctgatg
      301 cgcagcgtcc agtcgtcggc cgccgcgccc ggagccgcca cgcgcagcag ctcgcgcgtc
      361 gtgccgccgc cgttcttcca cgggctcgcg gcgacgtcga cggcgcggat gaagcgcagg
      421 cccggcgggg cggccgggcc ggcggcgcgg gcggcgagct cgtccagctt cacggcaggt
      481 ccctcgcctc gcggcctgcc gtgcgggctt cccgggaagc ggtcgggcgg tcgctcatgg
      541 cttgaacgcg ctctcgagcg cgtaccgcga gccgggatgc acgagccgcg cgagcgtcac
      601 cggcaggccg cggctgacgg tgcggcgcac gacgacgagg cacgggtcgg tgcgcgcgat
      661 gcccaggagc ctggcctcct gggccgtcgg cagcgcggcc tcgatcgaga cctgggcttc
      721 ccacagcggc gcgacgtcga gcaggtagtg cgtcggcgtg cgctgcgtga agtcctgcgc
      781 gaggtagtcg ggcgcgctcg ccgggttgac gtagcggtcc tcgcactgca gcgggacgcc
      841 gtgctcgtgg tgcacgatca gcgtgtggaa gacgggcgcg ccggtcgcca ggcccaggcg
      901 ctgcgccagc gcggcggggg cggcctcggc gcgctgcagg tggacggtcg cgtcgtggcg
      961 gtggccgcgc gactggatct cctcctggat gtcgttgatc gtcagcgtcg acgacacgcg
     1021 attgagctgc gccgcgaacg tgcccacgcc ctggagccgc gtgatgaagc cggcgtcctg
     1081 cagctcgcgc agcgcgcggt gcaccgtcat gcggctgacg ccgaactgcg cgaccagctc
     1141 cgcctccgac ggcatctgct ggcccggcgc ccagcggccc tgcgcgagcg catccttcag
     1201 gaactgcttg acgcgcgcat agggcggctg cggcggcgac ttcgacgacg ggaggccgcc
     1261 cgcggcatgg tcgccgggcg aggcgcgcag gccgagcgcg gccggcgccg cggcggtgcg
     1321 cagtcgcgag cggggcgaag gaggcgagga cgacatgcgt ccaatgctac ttgcacaaac
     1381 ttgtctagac aagtagagtc gacccgcatt ggacgctggc gttccctcgc gtccgtccgc
     1441 gacgtccatc ccatgccgcg cgcgccgggc gtccgcccga ggcgggcgcg agcgctcact
     1501 gccggagaca tccccgtgac cgacctgtcc aacgcccacg ccctgccgcg ccccgtgcgc
     1561 gcgccgcgcg gcaccgagct cgcctgccgc aactggctcg tcgaggcggc ctaccgcatg
     1621 atccagaaca acctcgaccc cgaggtcgcc gagaacccga acgcgctggt cgtctacggc
     1681 ggcatcggca aggcggcgcg caactgggac tgcttcgagg ccatcctcga ggcgctgagg
     1741 aagctcggcg aggacgagac gctgctcgtg cagtcgggca agccggtggg cgtcttccgc
     1801 acccacgccg acgcgccgcg cgtgctgctg gccaactcga acctggtgcc caagtgggcg
     1861 acctgggagc acttccacga gctcgaccgc cggggcctga tgatgttcgg ccagatgacg
     1921 gcgggctcgt ggatctacat cggctcgcag ggcatcgtgc agggcaccta cgagaccttc
     1981 gtcgaggccg gccgccagca ctacggcggc tcgctcgccg ggcgctggat cctgaccgcc
     2041 ggcctgggcg gcatgggcgg cgcgcagccc ctggccgcga gcttcgcggg cgcgagctcg
     2101 ctgacgatcg agtgccagca gagccgcatc gacttccgcc tgaagacgcg ctacgtggac
     2161 gagcaggccg cggacctcga cgacgcgctc gcccgcctcg cgcgctacac ggccgaaaag
     2221 cgcgccgtct cgatcgcgct gctgggcaac gccgccgagg tgctgccgga gctcgtgcgg
     2281 cgcgcgcgcg ccggcggccc gcggcccgac ctcgtgaccg accagacctc cgcgcacgac
     2341 ctcgtcaacg gctacctgcc cgtcggctgg agcgtggcgc agtggcgtga ggccgcggcc
     2401 gaccacgcac gccatgccga gctgcgcgac gccgccgcga agggctgcgc ggtgcacgtg
     2461 caggcgatgc tggacttcca ggcgatgggc gtcccgaccg tcgactacgg caacaacatc
     2521 cgccaggtcg cgttcgacca gggcgtcgcc aacgcgttcg acttccccgg cttcgtgccg
     2581 gcctacgtgc gcccgctgtt ctgccagggc aagggcccgt tccgctgggt cgcgctctcg
     2641 ggcgacccgg aagacatccg caggaccgac gcgaagatga aggagctgtt ccccgaggac
     2701 gcgcacctgc accgctggct cgacatggcc ggcgagcgca tcgcgttcca gggcctgccg
     2761 gcgcgcatct gctggatcgg actcggcgac cgcgaccgcg ccggccgcgc gttcaacgag
     2821 atggtccgca cgggcgagct gaaggcgccg atcgtgatcg gccgcgacca cctggattcg
     2881 ggctccgtgg cctcgcccaa ccgcgagacc gagtcgatga aggacggctc ggacgcggtg
     2941 tccgactggc cgctgctcaa cgcgctgctc aacaccgccg gcggcgcgac ctgggtctcg
     3001 ctgcatcacg gcggcggcgt cggcatgggc tactcgcagc acgcgggcgt cgtgatcgtc
     3061 tgcgacggca cgaaggaggc cgacgcacgc ctagcgcgcg tgctctggaa cgatccggcc
     3121 accggcgtga tgcgccacgc cgacgccggc tacgacgacg cccgcgccgc cgcgaagcgc
     3181 catggcctga acctgccgat gatcaagtga acgcgatgac ccagacgctc ctgctcaagc
     3241 ccggccagct cacgctcgcc gacctccagg ccctgcacgc gggcggcgtg cgcctcgcgc
     3301 tcgcgcccga ggcccgcgcc gcgatcgccg ccagcgccgc ggtcgtgcag gccgcggcac
     3361 tcggcgacgc gccggtctac ggcgtcaaca cgggcttcgg caagctcgcg aacaagcgca
     3421 tcagccagga cgagctcgac gcgctgcagc gcaacctgat ccgctcgcat tcggtgggcg
     3481 tgggcgagcc gctgcagccg gcggtcgtgc ggctgatgct ggcgatcaag gcggcgagcc
     3541 tcgcgcgcgg gcactcgggc gtgcgcgagg cagtcgtcga cacgctggtc gacgcgtaca
     3601 acgcgggcct cgtgccctac gtgccgtcgc agggctcggt cggcgcctcg ggcgacctcg
     3661 cgccgctgtc gcacatgacg ctggcgctga tgggcgaggg cgagatgctg gtcgacggcc
     3721 gccgcgtgcc ggcgctgccc gagctggaga aggccggcct cgcgccgctg cggctcgagg
     3781 cgaaggaagg gctcgcgctg atcaacggca cgcagacctc gaccgcgctg gcgctgcacg
     3841 cgctgatctc gttcgagccg gtgctggagt cggcgctcgt gatcggcgcg ctgaccgtgg
     3901 acgcggcgcg cggcagcgac gggccgttcg acccgcgcat ccacgcgctg cgcggccagc
     3961 cggggcagat cgacgtcgcg cgctactacc gcgcgctgct cgcgggcagc gagatccgcc
     4021 gctcgcacct cgagggcgac gaccgcgtgc aggatccgta ctgcctgcgc tgccagcccc
     4081 aggtcgtcgg cgcctgcctc gaccagctgc gccacgccgc gctcgtgctg gtgcgcgagg
     4141 ccaacgccgt gaccgacaac ccgctcgtgt tcgccgaaga cggcgtgctg gtctcgggcg
     4201 gcaacttcca cgccgagccg gtcgcgctcg cctgcgacgc gatggccgtc gcgatcgccg
     4261 aggtcggcgc gatcgccgag cgccgcatcg cgatgctgat cgacgccggc gtctcgcgcc
     4321 tgccgccgtt cctgtcggcg gacgccggcc tgaactcggg cttcatgatc gcccacgtga
     4381 ccgcggcctc ggtcgcgagc gagaacaagt cgctcgcgca tccggccagc gtcgactcgc
     4441 tgccgacctc ggcgaaccag gaagaccacg tctcgatggc caccttcgcc gcgcgccggc
     4501 tgcaggcgat gatcgccaac gtcgcgacca tcctcggcat cgagtggctc gcgagcgcgc
     4561 agggcgtcga gttcctgcgg ccgctgcgca gctccgcggc gctggagcag gcgcatgcgc
     4621 tgctgcgcga gcagtgcgcg gcgatgccga ccgaccgctg gctcgcgccc gacatcgagc
     4681 gcgcgaccgc gctcgtggcg ggcgggtcgc tctcgcgcgt gttccgcacg ctcgaggggc
     4741 tgccggccct gtggacgccg gcctgaacgg ccgggcgaca gcgccggtgt cgccgacgcg
     4801 acatcgggcc tcggcgccag aaaagtctcg ttccgggccg tttgcgcggg ccttgcccgg
     4861 ccgggggtgg gatttccgtc catttttgcc gttcggccgg tgagcgcggg cccgacaatg
     4921 tcgcgcatgt ccaaggtccc gcccgagacg cccggctacg aggcgaacgg ccgcccggct
     4981 gcgcgcctgc gggcgctgct gcagcgctgg atgcgctcgc ccgcggcggg gcgcgacgtg
     5041 ctgggcgagt accgcgaccg catcatgctg cccatggccg cggcgtccgt ggcgctgctg
     5101 ctgccgttcg cggcctggga cttcctctcg ggcaagtggc tgctgggcgc cctcgcgttc
     5161 gcggtgtgcg tgccgctcgc gctcgacgcg gccgcgctgc accgccgccg caagcccccg
     5221 gtggcctacg gctggatcct gctgcccatc gccgcggcga tcgcggcggc gctgctcgcg
     5281 cgcggggtgc acggcgcgat gtggtgctat ccggcgatcc tgttctgcta cttcgtgctg
     5341 cccggctggc aggcgaccgc cggggcgctg ggcctgatgg cgatgacctc ggcgctggcc
     5401 tggcacgtcc tgggcgcgcc gacgacggtg cgcttcgcgg tgacctcggg cctgtcggtg
     5461 atcatcctga acatcatcct gggcgtcgtc ggcgagctgc agaagaagct ctacgagcag
     5521 gcgaccaccg acccgctcac cggcgcgctc aaccggcgcc agctcgcgca gacgctcgcc
     5581 gacgcgatcg agcattcgcg ccggctcgag gcgccggtgt cgctgctggc cttcgacctc
     5641 gaccacttca agcgcgtgaa cgaccgccac ggccacgccg cgggcgacgc ggtgctgcag
     5701 cgcgtcgtcg gcctggtgca ggagcgctcg cgccgcatcg accggctgtt ccgcatgggc
     5761 ggcgaggagt tcgcgctgct cctgcccggc accaacgtcg ccgacgcgat gcgcgtggcg
     5821 agcgtgctgc gcgagcgcgt ggccggcgcc gactggttcg acgacgcgcg catcacggtg
     5881 agcgtcggcg tcgcccagct gcaccgcaag cacacggtcg actcgtggct gaagtcggcc
     5941 gacgacgcgc tctaccgcgc gaaggtggac ggtcgaaact gcgtgcgcgt cgccacgcgc
     6001 agccgcacca acgccgcggg cgagctggtc gacaccgggt tcagccggcc gagcgacctc
     6061 gtgccgctgg tgcccgccgc gcgcgactag ccttcgcggg gaaggccgac gagggcggct
     6121 cgcggccggt gctcccggca ggcagcctcc cggccgccct cacaccgccg ccgcgaccac
     6181 ctcgcgcagc cagcggtgcg ccgggtcgcg gtgcacgcgt tcgtgccaca ccatggccat
     6241 ctcgtagccg ggcacctcca ccggcggctc gacgacgcgc aggccgggcg tcgcgcgcac
     6301 gaggcgctcg ggcagcacgg cgaccaggtc ggtctcccgc agcgccgcgc gcaggaacag
     6361 gaagtgcggc accgacagca cgacgagat
//
LOCUS       DASVLN010000777         4560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_362533, whole genome shotgun sequence.
ACCESSION   DASVLN010000777 DASVLN010000000
VERSION     DASVLN010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362533"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VF453_18410"
     CDS             complement(<1..605)
                     /locus_tag="VF453_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689685.1"
                     /translation="MAAPPASTGPAFLRWPIVQTIRLQLRFLLPLAAVVIVTAYMVQP
                     LVDRVSLRWFARDLDSRGAVVANALSDSVIEAMRAGRPQQLEKLFDRAAHDERLFAIG
                     LCSTDGRMLVKTPAFPPEINCFNAQEVSAKRDPRFMLPGGPVHVAMHDVVDPPHPIAA
                     PASAASAASAAQSAASIAAAEAAASAASAAASAVEKHRGRHR"
     gene            678..2498
                     /locus_tag="VF453_18415"
     CDS             678..2498
                     /locus_tag="VF453_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7689686.1"
                     /translation="MSSLPVASSAPAASLDLAMIGNCSISALIDGTAHVVWCCMPRFD
                     SPPVFDALLQSANGVPEQGTMSVRIEGLARTEQSYDPGTAILRTRLWDAQGQGIEIVD
                     FAPRFVTRDRVFRPQQLVRRIRPLAGRPRVRVTVRPHGDWGAEPPLVTRGSHHVRFVL
                     PGITLRLNTSVPITYLMDGTWFALDREMSLFLGPDETMSGGIDETAREFEDQTSLHWR
                     HWTHRLALPLEWQDAVIRAAITLKLCQYEETGAIVAAMTTSLPEAPNSGRNWDYRFCW
                     LRDAFFVVRALNGLSEVGTMEKYLGWLTNVLHAEAGGHVQPLYGIGLERQLAESVVAQ
                     AQGYRGMGPVRVGNQAHEHFQHDTYGNIILAAAQSFHDHRLFRRGDASDFAALEAMGE
                     QAWRLHDQPDAGIWELRTRARIHTSSSLMCWAACDRLANIALTVGAAERAAFWRDRAA
                     VVRGKILQFAWNEKRQAYVESFGGSELDASVLLMGEVGFLPPKDPRFISTVEAMERTL
                     CDGHFMRRYEAADDFGRPETTFNICSFWRVDALARIGRRDEAREAFEALLARRNPAGI
                     LSEDMNPATGELWGNFPQTYSMVGIIHGALRLSAPWDSVV"
     gene            2918..3676
                     /locus_tag="VF453_18420"
     CDS             2918..3676
                     /locus_tag="VF453_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7689687.1"
                     /translation="MQIPSLKDRVSAEEWALRCDLAACYRLVAAHGWSDLVFTHISAR
                     LPGGDHAFLINPYGLMFEEVTASSLVVVDQDGNKRDDSPFPVNRAGFVIHSAIHAARE
                     DAGCVLHTHTRAGVAVSAQRGGLLPISQQSTFVMGALAYHDYEGVAMHDAEKPRLQAD
                     LGRARFLMLRNHGLLTVGRTVADAFLSMYLFEGACQIQLAAQAGGGPLVTIGQEILDG
                     VAASMRVQTEGQGAAFVWPALLRKCERMDPGYRS"
     gene            complement(3693..4250)
                     /locus_tag="VF453_18425"
     CDS             complement(3693..4250)
                     /locus_tag="VF453_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689688.1"
                     /translation="MPVFEIKPVFIDGIDAAAEWVDDEHSTWNEPRLRTTARLAGHWV
                     APSLELYRSEARATPVLFHPTVHVVSQGVRDELSHFAELEFLPVHVAGFGTFHAVHVL
                     ASCELPAGSLVDRGPGTDIALIRAFPRAFVPPAPFFRIRHPGAANAGGVAVCVPDIYA
                     SEAGAAAVQRVAGRFLRAVRVPYSD"
BASE COUNT          672 a         1634 c         1630 g          624 t
ORIGIN      
        1 cggtggcggc cgcggtgctt ttcgaccgcg gacgccgccg cggacgccgc gctcgcggcg
       61 gcctcggcgg ccgcgatcga ggccgcggac tgcgccgcgg aagcggccga ggcggcgctc
      121 gccggcgcgg cgatcggatg cggcgggtcg accacgtcgt gcatcgccac gtgcaccggg
      181 ccgccgggaa gcatgaagcg cgggtcgcgc ttggcgctga cctcctgcgc gttgaagcag
      241 ttgatctccg gggggaaagc cggcgtcttc acgagcatgc gaccatcggt gctgcacagc
      301 ccgatggcaa acagtcgctc gtcgtgcgcc gcgcgatcga acaacttctc cagctgctgc
      361 ggacggcccg cgcgcatcgc ctcgatgacg gagtcggaca gcgcgttggc gacgacggcg
      421 ccgcggctgt cgaggtcgcg cgcgaaccac cgcagcgaga cgcggtccac cagcggctgc
      481 accatgtagg ccgtgacgat gacgacggcc gcaagcggca gcaggaagcg cagttgcagc
      541 cgtatcgtct ggacgatggg ccatcgcagg aaggcaggcc cggtacttgc cggcggggcg
      601 gccaaagacc ccgggtttct atctttgttc aagagagtga tccactgttc gctttcaaat
      661 ccaaggcccc cacgcacatg agcagcctcc ccgtcgcctc gagcgcgccc gccgcctcgc
      721 tggatctcgc gatgatcggc aattgcagca tcagtgccct gatcgacggc acggcccacg
      781 tggtgtggtg ctgcatgccg cgattcgaca gtccgccggt attcgacgcg ctgctgcagt
      841 cagcaaacgg cgtgcccgag cagggaacga tgtccgtgcg catcgagggg ctcgcgcgca
      901 ccgagcaatc ctacgacccc ggcaccgcga tcctgcgcac aaggctttgg gacgcgcagg
      961 gtcaaggcat cgagatcgtc gattttgcac cgcgcttcgt cacgcgggac cgtgtgttcc
     1021 ggccgcagca gctcgtccga cgcatccgtc cgttggccgg ccggccgcgc gtgcgggtga
     1081 ccgtgcgccc gcacggcgac tggggcgcgg agccgccgct ggtcacgcgc ggcagccacc
     1141 acgtgcgctt cgtgctcccg ggcatcacgc tgcgactgaa caccagcgtg ccgatcacct
     1201 acctgatgga cggcacgtgg ttcgccctgg accgcgagat gagcctgttc ctcggccccg
     1261 acgagaccat gagcggcggc atcgacgaga cggcgcgcga gttcgaggac cagacctcgc
     1321 tgcactggcg ccactggacg caccgcctcg cgctgccgct cgagtggcag gacgccgtga
     1381 tccgggcggc gatcacgctc aagctgtgcc agtacgagga gaccggcgca atcgtcgccg
     1441 cgatgacgac gagcctgccc gaggcgccga acagcggccg caactgggac taccgcttct
     1501 gctggctgcg cgacgcgttc ttcgtggtcc gggcgctcaa cggattgtcc gaggtcggga
     1561 cgatggagaa gtacctcggc tggctcacca acgtgctgca cgcggaggcg ggcggccacg
     1621 tgcagccctt gtacggcatc ggcctggagc gccagctggc ggagtccgtc gtggcgcagg
     1681 cgcagggcta tcgcggcatg gggccggtgc gcgtgggcaa ccaggcgcac gagcacttcc
     1741 agcacgacac ctacggcaac atcatcctcg cggcggcgca gtccttccac gaccaccgcc
     1801 tgttccgacg cggcgacgcg tcggacttcg ccgcgctcga ggcgatgggc gagcaggcct
     1861 ggcggctgca cgaccagccc gacgccggca tctgggagct gcgcacccgt gcgcgcatcc
     1921 acacctcgtc gtcgctgatg tgctgggcgg cctgcgaccg cctcgccaac atcgcgctga
     1981 ccgtcggcgc cgcggagcgc gcggccttct ggcgcgaccg ggcggccgtc gtgcgcggca
     2041 agatcctgca gttcgcgtgg aacgagaagc gccaggccta tgtcgagagc ttcggcggca
     2101 gcgagctcga cgccagcgtc ctgctcatgg gcgaggtcgg cttcctgccg cccaaggatc
     2161 cgcgcttcat cagcaccgtc gaggcgatgg agcgcacgct gtgcgacggc catttcatgc
     2221 gccgctacga ggcggccgac gacttcgggc ggccggagac gaccttcaac atctgctctt
     2281 tctggcgcgt ggacgcactc gcgcgcatcg gacggcgcga cgaggcgcgc gaggccttcg
     2341 aagcgctgct cgcgcgccgc aatcccgcag gcatcctcag cgaggacatg aaccccgcca
     2401 cgggcgagct ctggggcaat ttcccgcaga cctattcgat ggtgggcatc atccacggcg
     2461 cgctgcggct gtcagcgccg tgggattcgg tggtgtgagc gcggggcctc gcccggcgag
     2521 ccggacaccg ggctgcccgc cgccaccagc gtcccgatca atccgaggcg gcgccgccgg
     2581 cggcgttcgt ggcgatggcg gtcgcatggt ccgggcggtc gcgcggtctg ggcggtctgg
     2641 gtggtctgcg tagtctggac ggtctgggtg gtccgggcgg tcgcggcggc aaatcacgcg
     2701 ccagctgccc gcaaatgcag cacggggctt gacatcaagc atccatgcgg cctggcgagc
     2761 cgtcctcaca tgggacccac agagttgtcc acagccgccg tggacaacgt cacgggactg
     2821 tcacgggcgg cgccccccgg cacgatcggc cggcgggacg gtgcgtcgcg caccaccgcc
     2881 tgcaaaatgc gccgacagcc actctccgga gaccgccatg cagatcccgt ccctgaagga
     2941 ccgcgtcagc gccgaggagt gggcgctgcg ctgcgacctc gccgcgtgct accgcctcgt
     3001 cgccgcccac gggtggagcg acctcgtctt cacgcacatc agcgcccggc tgccgggcgg
     3061 cgaccatgcc ttcctcatca atccatacgg cctgatgttc gaggaggtca cggcctcctc
     3121 gctggtggtc gtcgaccagg acggcaacaa gcgcgacgac tcgcctttcc cggtcaaccg
     3181 cgcgggcttc gtgatccaca gtgcgatcca cgccgcgcgc gaggacgccg gctgcgtgct
     3241 gcacacgcac acgcgcgccg gcgtcgccgt gagcgcgcag cgcggcggcc tgctgccgat
     3301 ctcgcagcag tccaccttcg tgatgggcgc cctggcgtac cacgactacg agggcgtcgc
     3361 gatgcacgac gccgagaagc cgcgcctgca ggccgacctg ggccgcgcgc gcttcctcat
     3421 gctgcgcaac cacggcctgc tgaccgtggg tcgcacggtg gccgacgcgt tcctctcgat
     3481 gtacctgttc gagggcgcct gccagatcca gctcgcggcg caggccggcg gcggcccgct
     3541 cgtgaccatc ggccaggaga tcctggacgg cgtcgccgcc tcgatgcgcg tgcagaccga
     3601 gggccagggc gcggccttcg tctggccggc gttgctgcgc aagtgcgagc ggatggatcc
     3661 cggctaccgc agctgaccgc ggcggccggg cgtcaatcgc tgtagggcac gcgcaccgcg
     3721 cgcaggaacc tgcccgccac ccgctgcacc gccgccgcgc cggcctcgct ggcgtagatg
     3781 tcgggcacgc agaccgcgac cccgcccgcg ttggccgcgc ccggatggcg gatgcggaag
     3841 aacggcgccg gcggcacgaa cgcgcgcggg aaggcgcgga tcagcgcgat gtcggtgccg
     3901 ggtccgcggt cgacgaggct gccggcgggc agctcgcagc tcgccagcac gtgcacggcg
     3961 tggaaggtgc cgaagccggc gacgtggacc ggcaggaact cgagttcggc gaagtgcgac
     4021 agctcgtcgc gcacgccctg cgagacgacg tggaccgtcg ggtggaacag caccggcgtg
     4081 gcgcgcgcct cggagcggta cagctccagc gacggcgcga cccagtgccc ggccaggcgc
     4141 gcggtcgtgc gcaggcgggg ctcgttccag gtggagtgtt cgtcgtcgac ccactcggcg
     4201 gcggcgtcga tgccgtcgat gaagacgggc ttgatctcga agacgggcat gcgccgagtg
     4261 tgacacgcgg cgagcgacgc gcgcgccccg cgcgcgaggc agtcgcgccg ccgtcgagca
     4321 cggcggcccg actccggcgc cgcgaccgcc gcggcgcaag tagactcccg acgggatacg
     4381 cgttgccaca ggcgccggcg cgagaccggc ggcaacccac ggaaaccgca ttgagcacga
     4441 acagaccgga cgcagccctc cagcgcgcct tcgctgtcct cgtcgccctc gccctggccg
     4501 cgctcgccgc cgtcgggctg tgggcgcacg cccagtacgc cccgctcgac gaggcgacgc
//
LOCUS       DASVLN010000778         2974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_525684, whole genome shotgun sequence.
ACCESSION   DASVLN010000778 DASVLN010000000
VERSION     DASVLN010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525684"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..804)
                     /gene="queC"
                     /locus_tag="VF453_18430"
     CDS             complement(79..804)
                     /gene="queC"
                     /locus_tag="VF453_18430"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX7689689.1"
                     /translation="MTDPRTALVLFSGGQDSTACLAWALARFARVETIGFDYGQRHRI
                     ELDCRADVRGALAALRPEWAARLGEDHLLDLSLLGQVSDTALTSERAIELQANGLPNT
                     FVPGRNLLFLGFAATVAYRRGASLLVGGMCETDFSGYPDCRDNTLKAMQVALSLGLAA
                     PMTIDTPLMFLTKAQTWSLTEALGGEALVDVVVERTHTCYLGERGARHPWGYGCGQCP
                     ACLLRAAGWNEYRAGRGALPAAA"
     gene            941..1828
                     /locus_tag="VF453_18435"
     CDS             941..1828
                     /locus_tag="VF453_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689690.1"
                     /translation="MNEIFTPGGRARAAFVATAAAALAAGCGGSVVDDPGGTNAAVRL
                     NCLGQACIASDTLPTSRITAMLYVVDDGNRTQAQAGFNTDGSITHNVELEGDELDFVD
                     GSRRTRMALSLAGAFDFFGDLLTLGQPYLADVSPQPAAARDYAFELVRADGTLRSTVT
                     LPAPFDIAAPSANARYPITTGTVPVTLTAPEPRNDLFATYTCVDSNGNQSTTSATATG
                     GGAFSATDATGLHYAFSVPAMLATISFAGGSAPPGTVRSCNVTIHVVDTMPGLLAPGF
                     HPFSIIEATQTRQVQITLD"
     gene            complement(1838..>2974)
                     /locus_tag="VF453_18440"
     CDS             complement(1838..>2974)
                     /locus_tag="VF453_18440"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX7689691.1"
                     /translation="AAANAQDLAAATQAGVAGPLHDRLKLDAHALDTVIQGCLQLAEM
                     ADPIGEITGVAQRPSGIRVGRMRVPLGVFGMIYESRPNVTIEAASLAIKSGNACILRG
                     GSEAIRSNVALWRCVQRALADAGLPETAVQLVETTDRAAVGRLLAMPEFVDVVIPRGG
                     RGLIERVSREAKVPVIKHLDGNCHTYVDARVDLELAVRVTDNAKTQKYSPCNATESLL
                     VHAAQAAAFLPRIGAVFAAKGVEMRGDARALAVLRDARLAGAKLVEAQDSDWGEEYLA
                     PVISIKVVDSLDEAIAHINRHGSHHTDAILTTDHPSAMRFLREVDSASVMVNASTRFA
                     DGFEYGLGAEIGISTDKFHARGPVGLEGLTSQKWIVLGEGETRG"
BASE COUNT          417 a         1088 c         1074 g          395 t
ORIGIN      
        1 gcaccaggcg gttgtagcag ccgaccaccc agcccagcag caccagcccg agcgcgagct
       61 ggacgagcca gtgcgcgttc atgcggcggc cggcagcgcg ccgcggccgg cgcggtactc
      121 gttccagccc gcggcgcgca gcaagcacgc cgggcactgc ccgcagccgt agccccaggg
      181 gtggcgcgcg ccgcgctcgc cgaggtagca ggtgtgcgtg cgctcgacga cgacgtcgac
      241 cagcgcctcg ccgccgagcg cctcggtcag cgaccaggtc tgcgccttgg tgaggaacat
      301 cagcggggtg tcgatcgtca tcggcgcggc cagcccgagg ctcagcgcga cctgcatcgc
      361 cttgagcgtg ttgtcgcggc agtcggggta gccggagaag tccgtctcgc acatcccgcc
      421 caccagcagc gacgcgcccc ggcggtaggc gaccgtcgcg gcgaagccga ggaacagcag
      481 gttgcgcccc ggcacgaagg tgttgggcag gccgttggcc tggagctcga tcgcgcgttc
      541 ggacgtgagg gccgtgtccg agacctggcc cagcagcgag agatcgagca ggtggtcctc
      601 gcccaggcgc gcggcccatt cgggacgcag ggcggcgagc gcgccgcgca cgtcggcgcg
      661 gcagtccagc tcgatgcggt ggcgctggcc gtagtcgaag ccgatcgtct cgacgcgggc
      721 gaatcgcgca agcgcccagg cgaggcaggc ggtggaatcc tggccgccgg agaacaggac
      781 gagggcggtg cggggatcgg tcatggacgg tcggcggctg gaggaagggg acggccgcaa
      841 gcatagtcgc tcgcgcgcgg ccgcgcgccg cccgggcaga caagggccgg gtttgcggcg
      901 aagatgcgcg ccatccgatt catcccaggg agcacgaacg atgaacgaga tcttcacccc
      961 cggcggccgg gcgcgcgccg ccttcgtcgc cacggccgct gcggcgctcg ccgccggctg
     1021 cggcggctcc gtcgtcgacg accccggcgg cacgaacgcc gccgtgcggc tgaactgcct
     1081 cgggcaggcg tgcatcgcgt ccgacacgct gccgacgtcg cgcatcaccg cgatgctgta
     1141 cgtcgtcgac gacggcaacc gcacccaggc gcaggccggc ttcaacacgg acggctcgat
     1201 cacgcacaac gtcgagctcg agggcgacga gctcgacttc gtcgacggca gccggcgcac
     1261 gcgcatggcg ctctcgctcg cgggcgcgtt cgatttcttc ggcgacctgc tcacgctcgg
     1321 ccaaccctac ctcgcggacg tctcgccgca gcccgcggcc gcgcgcgact acgccttcga
     1381 gctcgtgcgc gcggacggca cgctgcgcag cacggtgacg ctgccggcgc cgttcgacat
     1441 cgccgcaccg agcgccaatg cgcgctaccc gatcacgacc ggcacggtgc ccgtcacgct
     1501 caccgcgccc gagccgcgga acgacctgtt tgccacctac acctgcgtcg actccaacgg
     1561 caaccagagc acgaccagcg cgaccgccac cggcggtggc gcgttctcgg cgaccgacgc
     1621 cacggggctg cactacgcct tcagcgtgcc cgcgatgctc gccaccatca gcttcgcggg
     1681 cgggagtgcg ccgcccggca cggtgcgctc gtgcaacgtc accatccacg tcgtcgacac
     1741 gatgccgggc ctgctcgcgc ccggcttcca cccgttctcg atcatcgagg cgacgcagac
     1801 ccggcaggtg cagatcacgc tcgactgagc acggccctca gccgcgggtc tcgccctcgc
     1861 ccagcacgat ccacttctgc gaggtcaggc cctccagccc gaccggcccg cgggcgtgga
     1921 acttgtcggt ggagatgccg atctcggccc ccaggccgta ctcgaagccg tcggcgaagc
     1981 gggtgctcgc gttgaccatc acgctggcgg agtcgacctc gcgcaggaag cgcatcgcgc
     2041 tcgggtggtc ggtcgtcagg atcgcgtcgg tgtggtgcga gccgtggcgg ttgatgtggg
     2101 cgatcgcctc gtccagggag tcgacgacct tgatgctgat gacgggcgcc aggtattcct
     2161 cgccccagtc cgagtcctgc gcctcgacca gcttcgcacc cgcgagccgc gcgtcccgca
     2221 gcacggccag cgcgcgcgcg tcgccgcgca tctcgacgcc cttggccgcg aacacggcgc
     2281 cgatgcgcgg caggaaggcc gcggcctggg cggcatgcac gagcagcgac tcggtggcgt
     2341 tgcaggggct gtacttctgc gtcttcgcgt tgtcggtgac ccggacggcc agctcgaggt
     2401 cgacgcgggc gtcgacgtag gtgtggcagt tgccgtccag gtgcttgatc accggcacct
     2461 tcgcctcgcg cgagacgcgc tcgatcaggc ccctgccgcc gcgcgggatg acgacgtcga
     2521 cgaactccgg catcgccagc agccggccga cggccgcgcg gtcggtggtc tcgacgagct
     2581 gcaccgcggt ctcgggcagc ccggcgtcgg cgagcgcccg ctgcacgcag cgccacaggg
     2641 cgacgttgga gcggatcgcc tccgagccgc cgcgcaggat gcaggcgttg ccgctcttga
     2701 tcgcgagcga ggcggcctcg atggtgacgt tcgggcggct ctcgtagatc atcccgaaga
     2761 cacccagcgg cacccgcatg cggccgacgc ggatgccgct cgggcgctgc gcgacgccgg
     2821 tgatctcgcc gatcgggtcg gccatctcgg cgagctgcag gcagccctgg atcacggtgt
     2881 cgagtgcgtg cgcgtcgagc ttcaggcggt cgtgcagcgg gcccgccacg ccggcctgcg
     2941 tggccgccgc gaggtcctgg gcgttggcgg ccgc
//
LOCUS       DASVLN010000779         6601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_201487, whole genome shotgun sequence.
ACCESSION   DASVLN010000779 DASVLN010000000
VERSION     DASVLN010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201487"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..5867
                     /locus_tag="VF453_18445"
     CDS             <1..5867
                     /locus_tag="VF453_18445"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEX7689692.1"
                     /translation="QAPHIALRWRAWAPSWLNVGLVGDKTGAGLHDIDFSVAAPGVRD
                     FAGNGAPRPLVVNFAAPVAPQLRIGHEAPDVAISPAITGRWIWSSATQLTFQPNADWP
                     LATRYEVTLGRKALAPHVDVKRRAFEFDSPAFTVRMETPQFYQDPTQPTLRRVVVQVD
                     FSHPVDTASFESRVHLDDGSARKFSVAYDKLKLQASLQSEPLPIPAETVPMKITIDAG
                     VVAAHGGEGKGTHDAIAQSVDVPGLYSLAVSEVSASIVSNDNGDPEHMLRVTATMPVH
                     ERAFAQAVHAWLLPVSDKPGGEAFAWSDPSEVTDAVLKRSTAMKLEAVPGEREVDESL
                     ALRMPQAEGGRFVYVQVDKGLATPGGYQLGAPRTEILQIKTFAPELTIMSRGSLLALS
                     GEKKLPLLVRDLPGVQVELARVLPQQLHLLVTQANGEFTKPQFFGRLGADALTERFER
                     KIPLQLRPGKTHYETLDFADYLKGPGGERRGVFLLQVRGYDPNAQARPAQPAQAEGGD
                     TGGDSGGDNPDASADSGDGSDNGGGGDGAPQVDPSTMTDGRLVVVTDLGLIAKRAVDG
                     TRDVFVQSIATGAPVAGATIEVWGRNGQVVTTAQTDATGRATLADLSAFVRERQPVLL
                     AARKDGDLSFLPLGGAASLGRGDRTLDMSRFDVGGLHSNGLPNQMTAFLFSDRGIYRP
                     GDTIHVGAVVKAANWAASAKDLPVEVEVTDARGLSVRRQTLVVGASGALEITHATQES
                     SPTGDWTITVSLPRRTSPGAPDAPAQVLGSTTVKVQEFMPDRTKVAAHLTAERAEGWV
                     SPANLGVDVDVQNLFGTPAQHRRVDATMTLSPTWPSFAAYPDYAFFEGRNGQQQTDDL
                     GSQESDTDGKTHFDLRLERFQSATYEAVVLVKAFEPEGGRSVSTEVQALVSDRPYLVG
                     VKTPDSLEYVSRNARREVHVIAIDPKAQRIAVDGLTLVRLEHKTLSVLVKQSNGLYRY
                     ESRAKDVTLSQVPFRIGAAGTALALDTSTPGNFGYEVRDATGLVLNRVAYAVAGAANL
                     TRSLDRNAELQIKLDKTDYQPGDTIELSVQAPYTGAGLITIERDKVYAHAWFKADKTA
                     SVQHIVVPKDFEGNGYVNVQFVRDPASDEIYTSPLSYGAVPFATGVERRRAHVKLTAS
                     EKVKPGETVKMTLTSDKPAKAFVFAVDEGILQVARYQTPDPLAFFFQKRALEVTTLQT
                     LDLVLPEFAKLMHGVAPGGDGASEAARQLNPFKRKRDKPVAFWSGLVDVNGTKEFSYP
                     VPDSFNGQLRVMAVVVDDDHVASASTKAIVRGDLVLLPTVPVSMTPGDQVDVGVGVAN
                     NIAGSGKDAPVKLALSVGAGLEVVGDAQQVLRISERGEASAVFHLRAKSGAAASLGSS
                     SVVFTATSGKSSARLSTDTSVRPASALVTLVQSGTAIGGGELKSQLNAYPNFATSELS
                     VSSTPWAFAATLMQYLQEYPYGCTEQITSQVVPAVLLAGQPELARDMAKARAGAGAAP
                     LDTKAAMDRWIAQLRARQSADGGIALWPGNGPDEFATMYAVQVLVEARERKLPVPADL
                     VAKADQFLQARLGSATASGGWAWRTQAEMAWVLTRQGIQVPAALANLRRVAATQKTPT
                     DIGNAYLAASFQAIKQDAAARELFQPVWDDLVARSRAHAPRTAWEWYDDPLSHDATVL
                     YLAAHWFPKSLQQLPASTWETMSKMIGNGWYDSHSTASTLLAVDGYAQAAAASARGQL
                     KASVVDAQGKVQPLPLLGELRTMAKAAVPPAAAAVRLANGGDLPVFYGWAQTGYERNL
                     PETANYHGIEITHVILDDKGKAITEAKIGDEVTVQVTVRSTDRDTVNQVALVDVLPGG
                     LEPVLQPDAAPQAGDQGDGGQGGDQDGGAQPAWRRRLGGTGTWALDYADVREDRVIFF
                     GNIDRSARTITYRARATNTGQFAHPAAYAEAMYERRLYGRSAAGHFAVAPVAKK"
     gene            5895..>6601
                     /locus_tag="VF453_18450"
     CDS             5895..>6601
                     /locus_tag="VF453_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7689693.1"
                     /translation="MSASSIAAAVRAGVSRVARAWPWLVAAALVLVGLRLWPHAPLRE
                     RLGSSTQVLAADGRLMRLALAGDQQYRLWTPLDRIAPVQVEAVLLYEDRGFWHHPGID
                     PLALARGAWNSAFGARREGGSTITMQLARRLYRIDSRTLRGKALQVAASLWLEARYGK
                     RELLEAYLNCAPYGGNIEGVGAASLIYFGHPAATLTLPEALTLAVIPQNPLKRLATAG
                     ARPDEPAAGAGAAPSAA"
BASE COUNT          989 a         2393 c         2397 g          822 t
ORIGIN      
        1 ggcaggcgcc gcacatcgcg ctgcgctggc gcgcgtgggc gccgagctgg ctcaacgtcg
       61 gcctcgtcgg cgacaagacc ggcgccggcc tgcacgacat cgacttcagc gtcgccgcgc
      121 ccggcgtgcg cgacttcgcc ggcaacggcg ccccgcggcc gctggtcgtg aacttcgccg
      181 cgccggtggc gccgcagctg cgcatcggcc acgaggcccc cgacgtcgcg atctcgccgg
      241 cgatcacggg ccgctggatc tggtcgagcg cgacgcagct cacgttccag ccgaacgccg
      301 actggccgct cgcgacgcgc tacgaggtca cgctcggccg caaggcgctc gcgccgcacg
      361 tggacgtgaa gcgccgcgcc ttcgagttcg actcgccggc cttcacggtg cgcatggaga
      421 cgccgcagtt ctaccaggac ccgacccagc cgacgctgcg ccgcgtcgtc gtgcaggtgg
      481 acttcagcca ccccgtggac accgcgagct tcgagtcgcg cgtgcacctg gacgacggca
      541 gcgcgcgcaa gttcagcgtg gcctacgaca agctgaagct ccaggcgtcg ctgcagtccg
      601 agccgctgcc catcccggcc gagaccgtgc cgatgaagat caccatcgat gccggcgtcg
      661 tcgcggcgca cggcggcgag ggcaagggca cgcacgacgc gatcgcgcag tcggtcgacg
      721 tgccgggcct gtacagcctt gcggtcagcg aggtgagcgc gagcatcgtc agcaacgaca
      781 acggcgaccc cgagcacatg ctgcgcgtga ccgccacgat gccggtgcac gagcgcgcct
      841 tcgcgcaggc cgtgcacgcc tggctgctgc cggtctccga caagcccggc ggcgaggcgt
      901 tcgcgtggag cgatccctcc gaggtcaccg acgccgtgct caagcgctcg accgcgatga
      961 agctcgaggc cgtgcccggc gaacgcgagg tcgacgagag cctggccctg cgcatgccgc
     1021 aggccgaggg cggccgcttc gtctacgtgc aggtcgacaa gggcctggcg acgccgggcg
     1081 gctaccagct cggcgcgccg cgcaccgaga tcctgcagat caagaccttc gcgcccgagc
     1141 tgacgatcat gagccgcggc tcgctgctgg cgctgtcggg cgagaagaag ctgcctctgc
     1201 tcgtgcgcga cctgccgggc gtgcaggtgg agctcgcgcg cgtgctgccg cagcagctgc
     1261 acctgctcgt gacccaggcc aacggcgagt tcacgaagcc gcagttcttc ggccgcctgg
     1321 gcgccgacgc gctgaccgag cgcttcgagc gcaagatccc gctgcagctg cgccccggca
     1381 agacgcacta cgagacgctg gacttcgccg actacctcaa gggcccgggc ggcgagcgcc
     1441 gcggcgtgtt cctgctgcag gtgcgcggct acgaccccaa cgcgcaggcc aggccggcgc
     1501 agccggcgca agccgaaggc ggcgacaccg gcggcgactc gggcggcgac aacccggacg
     1561 ccagcgccga cagcggcgac ggcagcgaca acggcggcgg cggcgacggc gcgccgcagg
     1621 tcgacccgtc cacgatgacc gacggccggc tggtcgtcgt caccgacctc gggctgatcg
     1681 cgaagagggc cgtcgacggc acgcgcgacg tgttcgtgca gtcgatcgcc accggcgcgc
     1741 ccgtggccgg cgcgacgatc gaggtctggg gccgcaacgg ccaggtcgtc acgaccgcgc
     1801 agacggacgc caccggccgc gcgacgctgg ccgacctctc ggccttcgtg cgcgagcgcc
     1861 agccggtgct gctggccgct cgcaaggatg gcgacctcag cttcctgccg ctcggcggcg
     1921 ccgcctcgct gggacgcggc gaccgcacgc tggacatgtc gcgcttcgac gtcggcgggc
     1981 tgcacagcaa cggcctgccc aaccagatga cggccttctt gttcagcgat cggggcatct
     2041 accggccggg cgacacgatc cacgtcggcg cggtcgtgaa ggccgcgaac tgggcggcga
     2101 gcgcgaagga cctgccggtc gaggtggagg tcaccgacgc gcgcggcctg tccgtgcggc
     2161 ggcagacgct ggtcgtcggc gcgagcggcg cgctcgagat cacgcacgcg acgcaggaga
     2221 gctcgcccac cggcgactgg acgatcacgg tgagcctgcc gcgccgcacg agccccggcg
     2281 cgcccgatgc cccggcgcag gtgctcggct cgaccaccgt caaggtgcag gagttcatgc
     2341 ccgaccgcac gaaggtcgcc gcgcacctga ccgccgagcg cgccgaaggc tgggtgagcc
     2401 ccgcgaacct gggcgtggac gtcgacgtgc agaacctgtt cggcacgccg gcgcagcacc
     2461 gccgcgtcga cgcgacgatg acgctgtcgc cgacctggcc gtcgttcgcg gcctatcccg
     2521 actacgcgtt cttcgagggc cgcaacggcc agcagcagac ggacgacctc ggctcgcagg
     2581 agagcgacac cgacggcaag acgcacttcg acctgcggct ggagcgcttc cagtccgcga
     2641 cctacgaggc cgtcgtgctg gtcaaggcct tcgagcccga gggcggccgc agcgtctcga
     2701 ccgaggtgca ggcgctcgtg agcgaccggc cctacctggt cggcgtgaag acgcccgact
     2761 cgctcgagta cgtgagccgc aacgcccgcc gcgaggtgca tgtcatcgcc atcgacccga
     2821 aggcgcagcg catcgcggtc gacgggctca cgctcgtgcg gctcgagcac aagacgctgt
     2881 cggtgctggt caagcagtcc aacgggctgt accgctacga gtcgcgggcc aaggacgtca
     2941 cgctgtcgca ggtgccgttc aggatcggcg cggccggtac cgccctggcg ctggacacgt
     3001 cgacccccgg caacttcggc tacgaggtgc gcgacgccac gggcctcgtc ctcaaccggg
     3061 tcgcctatgc ggtggccggc gcggccaacc tgacgcgctc gctcgaccgc aacgcggagc
     3121 tgcagatcaa gctcgacaag accgactacc agcccggtga caccatcgag ctgagcgtgc
     3181 aggcacccta caccggcgcg ggcctgatca cgatcgagcg cgacaaggtc tacgcccacg
     3241 cctggttcaa ggccgacaag acggcgtcgg tgcagcacat cgtcgtgccg aaggacttcg
     3301 agggcaacgg ctacgtcaac gtgcagttcg tacgggaccc ggcgtccgac gagatctaca
     3361 ccagtccgct cagctacggc gcggtgccgt tcgccaccgg cgtcgagcgg cggcgggcgc
     3421 acgtgaagct cacggccagc gagaaggtga agccgggcga gaccgtgaag atgacgctga
     3481 ccagcgacaa gcccgccaag gccttcgtgt tcgcggtcga cgagggcatc ctgcaggtgg
     3541 cgcgctacca gacacccgat ccgctcgcgt tcttcttcca gaagcgcgcg ctcgaggtca
     3601 cgacgctgca gacccttgat ctcgtcctgc ccgagttcgc caagctgatg cacggcgtcg
     3661 cgccgggcgg cgacggcgcg agcgaggcgg cgcgccagct caacccgttc aagaggaagc
     3721 gcgacaagcc ggtggccttc tggtcggggc tggtggacgt caacggcacg aaggaattca
     3781 gctacccggt gcccgacagc ttcaacggcc agctgcgcgt gatggcggtg gtggtggacg
     3841 acgaccacgt cgcgtccgcc agcaccaagg ccatcgtgcg cggcgacctc gtgctgctgc
     3901 ccacggtgcc ggtgtcgatg acgcccggcg accaggtgga cgtcggggtc ggcgtggcca
     3961 acaacatcgc cggttcgggc aaggacgcgc cggtcaagct cgcgctgtcg gtgggcgccg
     4021 ggctggaggt ggtgggcgac gcgcagcagg tcctcaggat cagcgagcgc ggcgaggcca
     4081 gcgcggtgtt ccacctgcgc gcgaagtccg gcgcggcggc gtcgctgggc tcgtcgagcg
     4141 tggtcttcac tgccaccagc ggcaagtcga gcgcgcgcct gtccaccgac accagcgtgc
     4201 ggccggcctc ggcgctcgtg acgctggtgc agagcggcac ggccatcggc ggcggcgagc
     4261 tgaagtcgca gctgaacgca tacccgaact tcgcgacgag cgagctctcc gtctcgagca
     4321 cgccctgggc cttcgcggcg acgctgatgc aatacctgca ggagtatccg tacggatgca
     4381 ccgagcagat cacgagccag gtcgtcccgg ccgtgctgct cgcgggccag ccggagctcg
     4441 cgcgcgacat ggccaaggcg cgcgcgggcg cgggggcggc gccgctcgac acgaaggcgg
     4501 cgatggatcg ctggatcgcc cagctgcggg cgcgccagtc ggccgacggc ggcatcgcgc
     4561 tgtggcccgg caacgggccc gacgagttcg ccacgatgta cgcggtgcag gtgctggtcg
     4621 aggcgcgcga gcgcaagctg ccggtgccgg ccgacctcgt cgccaaggcg gaccagttcc
     4681 tccaggcgcg cctcggctcg gcgaccgcca gcggcggctg ggcatggcgc acgcaggcgg
     4741 agatggcctg ggtgctgacg cgccagggca tccaggtgcc ggcggcgctc gccaacctgc
     4801 gccgcgtcgc ggcgacgcag aagacgccca ccgacatcgg caacgcctac ctcgccgcga
     4861 gcttccaggc gatcaagcag gatgccgccg cgcgcgagct gttccagccg gtgtgggacg
     4921 acctcgtcgc ccgcagccgc gcgcacgcgc cgcgcacggc ctgggagtgg tacgacgatc
     4981 cgctgtcgca cgacgcgacg gtgctctacc tcgccgcgca ctggttcccg aagtcgctgc
     5041 agcagcttcc cgcctcgacc tgggagacga tgtcgaagat gatcggcaac ggctggtacg
     5101 acagccattc gaccgcgagc acgctgctgg ccgtcgacgg ctatgcgcag gccgccgcgg
     5161 cgtcggcccg cgggcagctc aaggcgagcg tcgtcgatgc gcagggcaag gtgcagccgc
     5221 tgccgctgct gggcgagctg cgcacgatgg ccaaggccgc cgtgccgccc gcggccgcgg
     5281 cggtgcgcct ggccaacggc ggcgacctgc ccgtcttcta cggctgggcg cagaccggct
     5341 acgagcgcaa cctgcccgag acggcgaact accacggcat cgagatcacg cacgtgatcc
     5401 tcgacgacaa gggcaaggcg atcaccgagg cgaagatcgg cgacgaggtg accgtgcagg
     5461 tgacggtgcg ctcgaccgac cgcgacacgg tcaaccaggt ggcgctcgtg gacgtgctgc
     5521 ccggcggcct cgagcccgtg ctgcagcccg acgccgcgcc ccaggccggc gaccagggcg
     5581 acggcgggca gggcggcgac caggatggcg gcgcccagcc cgcgtggcgc cgccgcctcg
     5641 gcggcacggg cacctgggcg ctggactacg ccgacgtgcg cgaggatcgc gtgatcttct
     5701 tcggcaacat cgaccgcagc gcgcgcacca tcacgtacag ggcgcgcgcg accaacaccg
     5761 gccagttcgc ccacccggcg gcctatgccg aggcgatgta cgagcgacgc ctgtacggcc
     5821 gctcggcggc gggccacttc gcggtcgcgc cggtggcgaa gaagtagcgg cggggcgcgc
     5881 ggaaggtcgt cgcgatgtct gcctcgagca tcgccgctgc cgtgcgtgcg ggcgtgtccc
     5941 gcgtcgcgcg cgcgtggcca tggctcgtcg ccgcggcgct cgtgctcgtg ggcctgcgcc
     6001 tgtggccgca tgcgccgctg cgcgagcggc tgggcagctc gacgcaggtg ctcgccgccg
     6061 acgggaggct catgcggctg gcgctcgccg gcgaccagca gtaccgcctg tggacgccgc
     6121 tggaccgcat cgcgcccgtg caggtcgagg ccgtgctgct gtacgaggac cgcggcttct
     6181 ggcaccaccc cggcatcgac cccctggcgc tcgcgcgcgg tgcgtggaac agcgcgttcg
     6241 gcgcgcggcg cgagggcggc tcgacgatca cgatgcagct cgcgcggcgc ctgtaccgca
     6301 tcgacagccg cacgctgcgc ggcaaggcgc tgcaggtcgc ggcctcgctg tggctggagg
     6361 cgcgctacgg caagcgcgag ctgctcgagg cctacctgaa ctgcgcaccc tacggcggca
     6421 acatcgaggg cgtgggcgcg gcgagcctga tctacttcgg ccatcctgcg gccacgctca
     6481 cgctgcccga ggcgctgacg ctggcggtga tcccgcagaa tccgctcaag cggctcgcga
     6541 ccgcgggagc gcgccccgac gagccggccg cgggcgccgg cgccgcgcct tccgccgccc
     6601 a
//
LOCUS       DASVLN010000780         5571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_471935, whole genome shotgun sequence.
ACCESSION   DASVLN010000780 DASVLN010000000
VERSION     DASVLN010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471935"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VF453_18455"
     CDS             complement(<1..331)
                     /locus_tag="VF453_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689694.1"
                     /translation="MTDSFPTAAADVASTTDAGPPAWHWINRFAAFADATPDVRRDAE
                     TAPAPAPVAAPRRHAFGTELPAAPFPDPHWVATSEDCATLLGLPADWATRPDWRALDV
                     LTGRATWP"
     gene            432..2078
                     /locus_tag="VF453_18460"
     CDS             432..2078
                     /locus_tag="VF453_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HEX7689695.1"
                     /translation="MSVLMGQMMKQPLTVSSLIRHAQRHAGDVEVVSKRCEGDLHRSN
                     WAGVADRARRFAQALDALGVAPGERVGTLAWNGHRHVEIYYGSSGSGRVCHTVNPRLF
                     PEQIEWIVNDAEDAVLCFDLGFLPLVEKLAPHLRSVRHFVLMSDAAHLPAASAIPGLV
                     AFDSLLDAQDGDYAWPALDEDVAATLCYTSGTTGNPKGVLYSHRSIVLHSYAAALPDA
                     MNCSTRDVILPIVPMFHVNAWGIPYTAAMIGAKLVMPGPHLDGASLYELFESEGVTFS
                     AGVPTVWLGLLTYMKSKGLRFSTFRRTVIGGSACPPAMMRTLQDDYGVEVIHAWGMTE
                     MSPLGTLCKLQSKHLQLPLEQQRKLLETQGRVIYGVDMAIIDDEGRSLPWDGQAAGNL
                     VVRGPWVLERYFKSTESPLVAVDGEPGWFPTGDVAAIDADGFMTITDRSKDVIKSGGE
                     WISSIELENIAVAHPAVHEAAVIACKHAKWDERPLLVVVLKPGASATREELLGFFEGK
                     VAKWQVPDDVAFVAELPHTATGKLQKLKLRQQFRDHVLPA"
     gene            2252..3502
                     /locus_tag="VF453_18465"
     CDS             2252..3502
                     /locus_tag="VF453_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7689696.1"
                     /translation="MTLPRKPRALAARLVLAGLAAHGGAAFAQKGETVRIAWIDPLSG
                     LMAPIGENEVKSFQYVAGIVNQGNAAGVKFEIVPIDNKLSPAETVNGLKSAIDQGIRY
                     VTQGDGSSAAAALLDAVNKHNEREPGKEVLYLNYAAVDPDLTNGKCSYWHFRLDADTS
                     MKMEALTTFIKADKSIRKVYIIGQNYAHGVQVAKFAKADLAVKRPDIQIVGEDLHPLA
                     KVTDFAPYVAKIKASGADTVITGNWGSDLALLVKAAGEAGLNVRFFTYYANNSGTPTA
                     LGAAAAGRVYYVGYGHSNMGGPIRKMQDDFKKKFNDDFYVTDLYTELQLLSEGMARAK
                     STDPVKVAAALEGLKLKSYNGDIEMRKSDHQLQQGLYISVWQKTDAKNPYSVENTGYT
                     FAPVAAYDAYVASTPTSCQMKRPS"
     gene            3519..4535
                     /locus_tag="VF453_18470"
     CDS             3519..4535
                     /locus_tag="VF453_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7689697.1"
                     /translation="MDQVAINLLNGLSSGLLLFMLSAGLTLIFSMMGVLNFAHAGFYM
                     LGAYLGWSAAHLVGFWGALVVAPLAVGVLGAAFERSCLRRVHRHGHVAELLVTFGLGV
                     LILEVVQLVWGRSALAFPPPPALAGPAFTIVQAPGHGLALVAGRADASTCAAAACTTF
                     PATRAFMMAMALAMLAALWALLTRTRVGLVIQAALTHPEMTEALGHDVPRVFMLVFGA
                     GSALAGLAGVIGGATFVTEPQMAAAIGSILFVVVVVGGTGSLVGAFVGSLLIGLLQTM
                     PLTIDKSFADLAASAGWRLSPEGALYPLARLTLAQVAPILPYLLLVLVLLVRPRGLFG
                     KRDG"
     gene            4528..>5571
                     /locus_tag="VF453_18475"
     CDS             4528..>5571
                     /locus_tag="VF453_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7689698.1"
                     /translation="MAEARPFHFRPLAAARWATWGGYALVLAVAPLVFRGSLAQSLLT
                     QMGIAIIACLSYNILLGQGGMLSFGHAVYSGIGGFAAIHVLARIGAGGSHLPVSLVPL
                     VGGAAGLASAAALGWAATRKSGTTFAMITLGIGELAWAMAPMLPEFFGGEGGVTADRT
                     AGPAVLGVTFGLQRQVCWLVAGYCFACTALMFAFTRTPLGRLLNAVRDNPERVAFIGV
                     DPQHVRWLAFTIAGGFAGIAGGLAALNFELATAEVLGAPRSGAYLLFTFIGGSSAFFG
                     PILGGVLMVLATVVLSGLTQAWLLYLGLTFLVMVMAAPGGLASLVLANLRLAAHGRLR
                     PLLPGYAALSAASA"
BASE COUNT          757 a         1973 c         2011 g          830 t
ORIGIN      
        1 cgggccaggt cgcacggccg gtcagcacgt cgagcgcacg ccagtcgggg cgggtcgccc
       61 agtcggccgg aaggcccagc agggtggcgc agtcctcgct cgtcgccacc cagtgcgggt
      121 cggggaaggg cgctgcgggc agttcggtgc cgaacgcgtg ccggcgcggg gccgcgacgg
      181 gcgcgggcgc gggggcggtc tcggcatcgc ggcgcacgtc gggcgtcgcg tcggcgaacg
      241 cggcgaagcg gttgatccag tgccacgcgg gcgggccggc gtctgtcgtg gaggcgacat
      301 cggcggcggc ggtcgggaag ctgtcggtca tccgcgtcag tctagccagc cggctcgccg
      361 ggcacgtcga aggggcttac agttgcccgg caggcggcgc gagggtgccg cgacgacgaa
      421 ggagacaagg catgagcgtc ctcatgggcc agatgatgaa gcagccgctg acggtgtcct
      481 cgctgatccg ccacgcgcag cgccacgcgg gcgacgtcga ggtggtctcc aagcgctgcg
      541 agggcgacct ccaccgcagc aactgggccg gcgtcgccga ccgcgcgcgc cgcttcgcgc
      601 aggcgctgga cgcgctgggc gtcgcgccgg gcgagcgcgt cggcacgctg gcctggaacg
      661 gccatcgcca cgtcgagatc tactacggca gctccggctc gggccgcgtc tgccacacgg
      721 tcaacccacg cctgttcccg gagcagatcg agtggatcgt caacgacgcc gaggacgcgg
      781 tgctgtgctt cgacctcggc ttcctgccgc tggtcgagaa gctcgcgccg cacctgagga
      841 gcgtgcgcca tttcgtgctg atgtccgacg ccgcgcacct gccggcggcg agcgcgatcc
      901 ccggcctcgt cgcgttcgac tcgctgctcg acgcccagga cggcgactac gcgtggccgg
      961 cgctggacga ggacgtcgcc gcgacgctct gctacacctc gggcacgacg ggcaacccga
     1021 agggcgtcct ctacagccac cgctccatcg tgctgcactc gtacgccgcc gcgctgcccg
     1081 acgcgatgaa ctgctcgacg cgcgacgtga tcctgccgat cgtgccgatg ttccacgtca
     1141 acgcctgggg catcccgtac accgccgcca tgatcggcgc caagctggtg atgcccgggc
     1201 cgcacctgga cggcgcctcg ctgtacgagc tgttcgagtc cgagggcgtg accttcagcg
     1261 ccggcgtgcc caccgtctgg ctcgggctgc tcacctacat gaagtccaag gggctgcgct
     1321 tcagcacctt caggcgcacg gtgatcggcg gctcggcctg cccgccggcc atgatgcgca
     1381 cgctgcagga cgactacggc gtcgaggtca tccacgcctg gggcatgacg gagatgtcgc
     1441 ccctggggac gctgtgcaag ctgcagtcca agcacctgca gctgccgctc gagcagcagc
     1501 gcaagctgct cgagacgcag ggccgggtca tctacggcgt cgacatggcc atcatcgacg
     1561 acgagggccg ctcgctgccg tgggacggcc aggccgcggg caacctggtc gtgcgcgggc
     1621 cgtgggtgct ggagcgctac ttcaagtcca cggagtcgcc gctggtggcg gtcgacgggg
     1681 agccgggctg gttcccgacc ggcgacgtcg cggccatcga cgccgacggc ttcatgacca
     1741 tcaccgaccg cagcaaggac gtcatcaagt ccggcggcga atggatcagc tcgatcgagc
     1801 tcgagaacat cgccgtcgcc catcccgcgg tgcacgaggc cgcggtgatc gcctgcaagc
     1861 atgcgaagtg ggacgagcgg ccgctgctgg tggtcgtgct caagccgggc gcgagcgcca
     1921 cgcgcgagga gttgctgggc ttcttcgagg gcaaggtcgc gaagtggcag gtgcccgacg
     1981 acgtcgcctt cgtcgcggag ctgccccaca cggccaccgg caagctgcag aagctcaagc
     2041 tgcgccagca gttccgcgac cacgtgctgc ccgcctgaag gggcgccggt ccgggccgca
     2101 ggtcgcgcgg gcgcatgtcg ccggtgcgac atagccccgg gtagtcccgg aaacggcgcg
     2161 cgcgaccggc gcggtaccgt atcgtccgcc agtccgacaa cgaccggcgg acgcgccgcg
     2221 tgcgcgcgcc gccgcagccc aggagacaga catgacgctt ccccgcaagc cgcgcgcgct
     2281 cgccgcgcgc ctcgtcctgg ccggcctcgc ggcgcacggc ggcgccgcct tcgcgcaaaa
     2341 gggcgagacc gtgcgcatcg cctggatcga cccgctgtcg ggcctgatgg cgccgatcgg
     2401 cgagaacgag gtgaagtcgt tccagtacgt cgccggcatc gtcaaccagg gcaacgccgc
     2461 gggcgtgaag ttcgagatcg tgccgatcga caacaagctg agccccgccg agacggtcaa
     2521 cggcctgaag tcggcgatcg accagggcat ccgctacgtg acgcagggcg acggctcgtc
     2581 ggccgcggcg gcgctgctgg acgcggtcaa caagcacaac gagcgcgagc ccggcaagga
     2641 ggtgctctac ctgaactacg ccgcggtcga ccccgacctg acgaacggca agtgcagcta
     2701 ctggcacttc cgcctcgacg ccgacacgtc gatgaagatg gaagcgctga ccaccttcat
     2761 caaggcggac aagtcgatcc gcaaggtcta catcatcggc cagaactacg cccacggcgt
     2821 gcaggtggcc aagttcgcga aggccgacct cgcggtcaag cggcccgaca tccagatcgt
     2881 cggcgaggac ctgcacccgc tcgccaaggt gaccgacttc gcgccctacg tggccaagat
     2941 caaggcctcg ggcgcggaca cggtgatcac cggcaactgg ggctccgacc tcgcgctgct
     3001 ggtcaaggcc gccggcgagg cggggctgaa cgtgcggttc ttcacctact acgcgaacaa
     3061 ctcgggcacg cccaccgcgc tcggcgccgc ggccgcgggg cgggtgtact acgtggggta
     3121 tggccattcc aacatgggcg gcccgatccg caagatgcag gacgatttca agaagaagtt
     3181 caacgacgac ttctacgtca ccgacctcta cacggagctg cagctgctgt ccgaggggat
     3241 ggcgcgcgcg aagtccaccg acccggtcaa ggtcgcggcc gcgctcgagg gcctgaagct
     3301 gaagagctac aacggcgaca tcgagatgcg caagagcgac caccagctgc agcagggcct
     3361 gtacatctcg gtctggcaga agaccgacgc gaagaacccg tactcggtcg agaacacggg
     3421 ctacaccttc gcgcccgtgg cggcctacga tgcctacgtc gcgagcacgc cgacgtcctg
     3481 ccagatgaag cgtccgtcct gagcgcccga tcccgcgcgt ggaccaggtc gccatcaacc
     3541 tgctcaacgg cctcagctcg gggctgctgc tgttcatgct cagcgccggg ctgacgctga
     3601 tcttcagcat gatgggcgtg ctgaacttcg cgcacgcggg cttctacatg ctcggcgcgt
     3661 acctgggctg gtcggcggcc cacctggtgg gcttctgggg cgcgctggtc gtcgcgccgc
     3721 tggcggtggg cgtgctcggc gcggcattcg agcgcagttg cctgcgccgc gtgcaccggc
     3781 acgggcatgt ggcggagctg ctcgtgacct tcgggctggg cgtgctgatc ctcgaggtcg
     3841 tgcagctggt ctggggccgc tcggcgctcg cgttcccgcc gccgcccgcg ctcgcggggc
     3901 cggcgttcac gatcgtgcag gcgcccgggc acgggctcgc gctcgtcgcc ggccgcgccg
     3961 acgcgtcgac ctgcgccgcc gcggcctgca ccaccttccc cgccacccgt gcctttatga
     4021 tggcgatggc gctggccatg ctcgcggcgc tgtgggcgct gctcacgcgc acgcgcgtcg
     4081 ggctcgtgat ccaggccgcg ctcacgcatc cggagatgac ggaggcgctg ggccacgacg
     4141 tgccgcgcgt gttcatgctc gtgttcggcg ccggcagcgc gctcgccgga ctggccggcg
     4201 tgatcggcgg cgcgaccttc gtcaccgagc cgcagatggc cgcggccatc ggctcgatcc
     4261 tgttcgtcgt ggtggtcgtc ggcggtaccg gctcgctggt cggcgcgttc gtcggctcgc
     4321 tgctgatcgg gctgctgcag acgatgccgc tgacgatcga caagtccttc gccgacctcg
     4381 cggcgagcgc cggctggcgg ctctcgcccg agggcgcgct gtacccgctc gcccggctga
     4441 cgctggcgca ggtcgcgccc atcctgccgt acctgctgct cgtgctcgtg ctgctggtgc
     4501 gaccgcgcgg cctgttcggg aagcgcgatg gctgaggcgc gaccgttcca cttccggccg
     4561 ctcgccgccg cgcgctgggc gacctggggc ggctacgcgc tggtgctcgc ggtcgcgccg
     4621 ctcgtcttcc ggggcagcct cgcgcagtcg ctcctgacgc agatgggcat cgcgatcatc
     4681 gcctgcctgt cgtacaacat cctgctcggg cagggcggca tgctcagctt cgggcatgcg
     4741 gtctacagcg ggatcggcgg cttcgcggcg atccacgtgc tcgcgcgcat cggcgcgggc
     4801 ggctcgcacc tgccggtgag cctggtgccg ctggtcggcg gggccgcggg gctcgcgagc
     4861 gcggcggcgc tgggctgggc ggcgacgcgc aagtccggca cgaccttcgc gatgattacg
     4921 ctgggcatcg gcgagctcgc gtgggcgatg gcgccgatgc tgccggagtt cttcggcggc
     4981 gagggcggcg tcacggccga ccgcaccgcc gggccagcgg tgctgggcgt caccttcggg
     5041 ctgcagcgcc aggtctgctg gctggtcgcc ggctactgct tcgcctgcac cgcgctgatg
     5101 ttcgcgttca cgcgcacgcc gctcgggcgg ctgctgaacg cggtgcgcga caacccggag
     5161 cgcgtcgcct tcatcggcgt cgacccgcag cacgtgcgct ggctcgcctt cacgatcgcg
     5221 ggcggcttcg cgggcatcgc gggcggcctg gccgcgctca atttcgagct cgcgaccgcc
     5281 gaggtgctcg gcgcgccgcg ctcgggcgcc tacctcctgt tcacgttcat cggcggctcg
     5341 tcggcgttct tcggcccgat cctcggcggc gtgctgatgg tgctcgcgac ggtggtgctg
     5401 tccggcctca cgcaggcctg gctgctgtac ctcggcctca ccttcctggt gatggtgatg
     5461 gcggcgcccg gcggcctggc gagcctggtg ctcgccaacc tgcgcctggc cgcgcacggg
     5521 cggctgcgcc cgctgctgcc gggctacgcc gcgctctcgg ccgcgtcggc g
//
LOCUS       DASVLN010000781        11870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_362571, whole genome shotgun sequence.
ACCESSION   DASVLN010000781 DASVLN010000000
VERSION     DASVLN010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362571"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VF453_18480"
     CDS             <1..1045
                     /locus_tag="VF453_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7689699.1"
                     /translation="AVLPAGKRLDLRYELVPTRRGRVPFEPAALRVRSRLGLAELQLR
                     VGAAQSLQVYPNFAALARYAWLAGDRRLAEIGIKTYMARGAGTDFKQLGEYVPGMPTR
                     HIDWNASMRHRRPVVREFQDDRDQSVMFLLDCGRRMRADEGTASRPSAAGAAATTSDG
                     SHFDQALNAAMLLAYVALKDGDAVGAMTFGHEPSQQRHFAPAKGMAALNTLVARLHDI
                     EPSAMHSDYLAAARELMLRLPRRSLVVVLTNFRDEDGEELAAALRLMRTRHLVLLASL
                     RETVVAEIAGQPLDEPRAVAEVASAHWFERSRAAGFRRLAGRDQLLIDVEPARLAAAL
                     VNRYHAVKRARLL"
     gene            1219..4053
                     /locus_tag="VF453_18485"
     CDS             1219..4053
                     /locus_tag="VF453_18485"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX7689700.1"
                     /translation="MSDLAKSFEPAAIEARWAPLWEGSGIYEPTLDPARPSFSIQLPP
                     PNVTGTLHMGHAFNQTIMDSLTRYHRMRGFNTLWVPGTDHAGIATQIVVERQLQDKGQ
                     SRQELGRKNFVARVWEWKQESGSTITRQMRRMGDSVSWQREYFTMDEKLSRTVTETFV
                     RLYEEGLIYRGSRLVHWDPQLKSAVSDLEVNSEEEDGFLWLIRYPLADGSGSLTVATT
                     RPETMLGDTALMVNPEDERYAALVGKMVVLPLVGREIPVIADAYVDREFGTGVVKVTP
                     AHDENDYAVGQRHGLPIIGVLTLDAKINENAPEQYRGLDRFVARKRVVADLEAAGLLV
                     EVRKHKLMVPRCERTGQVIEPMLTNQWYVAVTKPGADGSSIADKAIRAVSSGEVKFYP
                     EQWVNTYNHWMNDLQDWCISRQLWWGHQIPAWYADDGRIFVARDDAEARAKAEAAGYT
                     GPLVRDEDVLDTWYSSALVPFSTLGWPEKTLEQELFLPSSVLVTGYDIIFFWVARMIM
                     MTTHFTGKVPFRDVYIHGLVRDSQGQKMSKSEGNVLDPVDLIDGIALEPLLDKRVVGL
                     RRPETAPKVREATKKEFPEGIPGYGADALRFTFAALASLGRNINFDSKRCEGYRNFCN
                     KLWNATKFVLMNCEGEDCGILEGDPKQCPPGHNAFSQADRWITSELQRTEAAVAQGFA
                     DYRLDNVANAIYQFVWTEFCDWYLEIAKAQLADAKAAGDIARQRGTRRTLVRTLETVL
                     RLLHPITPFVTAELWETVAPIAGRAPAAGQTIATASYPVAQLERIDEAACAWVAQLKA
                     VVGACRSVRKQMNLSDAERMPLLTHGDAAFVEAAAPLLKALAKVSEVRVIADEAAFTE
                     QTKLAPVAVEGALRLALHVEIDVEAERARLAKEVARLDGEIVKANAKLANENFVARAK
                     PEVVEQERARLASFVETLARVKAQLAQLGG"
     gene            complement(4116..4820)
                     /locus_tag="VF453_18490"
     CDS             complement(4116..4820)
                     /locus_tag="VF453_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689701.1"
                     /translation="MSRETSAAALRREVIAEVFAAPAPTPLREHRAIMRGLRVGWSPV
                     ESGAPDIDAVRPLRGDGPVAGLAARLLQTTDERLALRRLVETGRLLPGLLAGGVACAP
                     GRYAVPPETLAWLGAHAGTLGVEADGCFDLSVEHLALLRAANWRPVDEQDAREILGIE
                     APELWPMPAVDGKRPYGDMSFYPLDMAAILGQPFAHDANGHVVVDEAKAARLERLHGQ
                     TLVALQVMLANVPLEG"
     gene            complement(4884..5111)
                     /locus_tag="VF453_18495"
     CDS             complement(4884..5111)
                     /locus_tag="VF453_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX7689702.1"
                     /translation="MDANKEIDTRGLNCPLPILKAKKALADMQSGEILKVVATDPSST
                     RDFQAFARQTGNELVEQQQVAAEYIHFLRRR"
     gene            complement(5137..5733)
                     /locus_tag="VF453_18500"
     CDS             complement(5137..5733)
                     /locus_tag="VF453_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7689703.1"
                     /translation="MSGVDWKFCPQCAHALAPITLPEDGGPKERLRCANCGFTHWNNP
                     TPVLAAIVECSDRGGRVLLARNAAWPGRRFALITGFMEAGETPEDGIAREVREETNLV
                     AESVKLVGVWDFQRMNQVIVAYHVVAHGEIALSPELAEYKLFEPGDIVCWPAGTGYAM
                     AEWLRARGYEPRFMDWATREAQSRDAVDPGSAPADGRG"
     gene            complement(5744..6649)
                     /gene="cysM"
                     /locus_tag="VF453_18505"
     CDS             complement(5744..6649)
                     /gene="cysM"
                     /locus_tag="VF453_18505"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HEX7689704.1"
                     /translation="MPKYLTLEDAIGRTPLVRLQRVVPAEVAARGNVILGKLEGNNPA
                     GSVKDRPAMSMIRRAEERGQIQPGATLIEATSGNTGIALAMAAAIKGYRMVLVMPEDL
                     SVERVQTMKAYGAELVLTPKSGGMEYARDLALQMQADGKGLVLDQFANPDNPRIHYET
                     TGPEIWADTEGQVTHFVSAMGTTGTITGVSRFLKEKNPAIRILGAQPAEGSRIPGIRK
                     WPEAYQPKIYEPSRVDETIQVTQDDAEEMARRLAREEGLFGGISAAGACWAALQVAQR
                     LENATIVFVVCDRGDRYLSTGVFPA"
     gene            6762..6846
                     /locus_tag="VF453_18510"
     tRNA            6762..6846
                     /locus_tag="VF453_18510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6796..6798,aa:Leu,seq:caa)
     gene            complement(6987..8180)
                     /locus_tag="VF453_18515"
     CDS             complement(6987..8180)
                     /locus_tag="VF453_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689705.1"
                     /translation="MNAIKRLTEIVLSEPDRSPEAMEVRNAIWPEGAPFYEGPQLEDV
                     LSTFEPQDVAFFDEQNWSALDAIWMLSTGEAPCDQPSSDRKSFEEMLADEAAQADEQE
                     REWMETDDSFEWGESSDDRFVGRPESKALSPSPARRKVLDRIRKKYNRWLVAHSVMFA
                     GRAPVVDMSIRPMLLPLADTDAAKYLYPLNVHGWIRWATQFKLPVHRRFWEAHNAWRC
                     ARWQHGRQVAWLCWRDGDSAVPIRCAPIPSESTWHIPLAFEYEASLQLGVQVNFVHHW
                     KVVAQEVFEVAKGVRTRWGRVPNVIRRLSPKERQRTVGGRLFQRIRAAVDALASERGL
                     VADEGGRYVFGLTQAELIAELRRIDPMLSERADRTISAALPALAHGKPQTGRPRVSAR
                     EPRGR"
     gene            8264..8536
                     /locus_tag="VF453_18520"
     CDS             8264..8536
                     /locus_tag="VF453_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HEX7689706.1"
                     /translation="MISTNQWAQAHAAATGTRVTEVYLPSEGFIDEEVFCALLTVKRS
                     TFWQGIKDGRYPKPVKVSARKNGWPVAKVRALFASLAGQADAIHGA"
     gene            8552..10291
                     /locus_tag="VF453_18525"
     CDS             8552..10291
                     /locus_tag="VF453_18525"
                     /inference="COORDINATES: protein motif:HMM:NF017826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEX7689707.1"
                     /translation="MANKQHGESEILAALRERYGLRRTKDGLVAEVDSDVVKLSDRFH
                     VGGIARAADGRVLGVILQAGNGGARRLCIAPYQAIREPKQLASYFEYLGLVPPAELRE
                     LKILATYITALAGLRSRVVLAREGIHQVSCDGKRKPVAVIGREVIGAPADEFLALPSS
                     VSCYSSQGDLAGWQRGFKPLLLANPLMTLAACFALAVPLGSLLGFPLTGLLLAGASST
                     GKTTLARFVTSTFSAPREPHQWSGTANGIEALAALHADLPLSLDELGAGDIRQTLATI
                     YRVSGGRAKARATMTGQLQQGEPLRCALFATGEATLNQQANAAGTDVRAGHDVRMPTI
                     WVDEAHGVYTDIGKAADGAAYAERVAIAMQENYGVLLPAFVGAVVGDVDGARETAEGM
                     RTAILTQVAGVDVLHMTGVERRVLTAFTNWAIAGELAVAYGALPLKAGEPAAAIAYVF
                     AKWLARWRALAQGPSLAPLRNLSDFFKRNHHRFAPIKDWQDSASASGRLGYRRATASH
                     GDHFLVFRDVFEKDAAQKHGVDACVNALRDAGLLIAGKKGRTLLVRMPRSEDGERMAL
                     YAIRANVMFDTEE"
     gene            10614..11699
                     /locus_tag="VF453_18530"
     CDS             10614..11699
                     /locus_tag="VF453_18530"
                     /inference="COORDINATES: protein motif:HMM:NF016998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid replication protein"
                     /protein_id="HEX7689708.1"
                     /translation="MIDYYDIELNQIRLGPKEWRRIPGHEKPTRPPNRMHVKLKEKGW
                     AATQEALIPHDSLRLLVCPQKVLGHWNCHGSDDLRQVVLDTAPAMLRAMGLEVTQEIM
                     SDLVLGKYGIRKVHIAHQFWLDDRCIRDFLRAVRRRLNESHEPVPVFRGDGFMIGNRS
                     RTVEYVFYVKLREFLKYGLPFYRERLAHERAKGRPYGARTGLERDLQRIAAAAGPRLE
                     MRLGDHYFRKNRNFARGTGWAAATADGIYQQHLRKLELPPVVEAAPARVRARQRLKTA
                     LKTYLLWADGHPLNDIGTNVKTVASHRRIILQELGFDIRTPASAVFGDNLTVDARSVF
                     DWKNRVQAAKVDVDDLYWLGDPYEPAY"
BASE COUNT         1911 a         4170 c         3949 g         1840 t
ORIGIN      
        1 cgcggtgctg cccgcgggca agcgcctcga cctgcgctac gagctcgtgc ccacgcgccg
       61 cggccgcgtg ccgttcgagc ccgccgcgct gcgcgtgcgc tcgcgcctgg ggctggcgga
      121 gctgcagctg cgcgtcggcg cggcgcagtc gctgcaggtc tacccgaact tcgcggcgct
      181 cgcgcgctac gcctggctcg cgggcgaccg ccggctcgcc gagatcggca tcaagaccta
      241 catggcgcgc ggcgccggca ccgacttcaa gcaactcggc gagtacgtgc ccggcatgcc
      301 cacgcgccac atcgactgga acgcgtcgat gcgccaccgc cgccccgtcg tgcgcgagtt
      361 ccaggacgac cgcgaccagt cggtgatgtt cctgctcgac tgcggccgcc gcatgcgcgc
      421 cgacgagggc acggcgagcc gcccgtccgc cgcgggcgcg gccgcgacga cgagcgacgg
      481 cagccacttc gaccaggccc tcaacgccgc gatgctgctg gcctacgtcg cgctcaagga
      541 cggcgacgcg gtcggcgcga tgaccttcgg ccacgagccc tcgcagcagc gccacttcgc
      601 gcccgcgaag ggcatggccg cgctcaacac gctggtcgcg cggctgcatg acatcgagcc
      661 gagcgccatg cactccgact acctggccgc cgcgcgcgag ctgatgctgc ggctgccgcg
      721 gcgctcgctc gtcgtggtgc tgaccaactt ccgcgacgag gacggcgagg agctcgccgc
      781 cgcgctgcgg ctgatgcgca cgcgccacct cgtgctgctc gcgagcctgc gcgagaccgt
      841 cgtcgccgag atcgccggcc agccgctgga cgagccgcgc gccgtcgcgg aggtcgcctc
      901 cgcgcactgg ttcgagcgct cccgcgccgc cggattccgc cgcttggccg gccgcgacca
      961 gctcctcatc gacgtcgagc cggcccggct cgccgcggcg ctggtcaacc gctatcacgc
     1021 agtcaagcgg gcgcggctgc tctgagcgcg cgttccgggc cgcaaatgcc tggtttcgac
     1081 gccgggcccc ctcaagcggg ccgcctacaa tcgagcgcac cccggccaca gatgccccgt
     1141 cgcgcggaag acgtcctttc gcgtgcggcg gactcgcttt ccccggccaa tcccggcaaa
     1201 tcctccaacg agcccgtcat gtccgatctc gccaagtcct tcgaacccgc cgccatcgag
     1261 gcccgctggg cgccgctgtg ggaaggcagc ggcatctacg agcccacgct ggatccggcc
     1321 aggccgtcgt tctcgatcca gctgccgccg cccaacgtca cgggcacgct gcacatgggc
     1381 cacgcgttca accagaccat catggactcg ctgacgcgct accaccgcat gcgcggcttc
     1441 aacacgctgt gggtgccggg caccgatcac gcgggcatcg cgacgcagat cgtggtcgag
     1501 cgccagctgc aggacaaggg ccagtcgcgc caggagctgg gccgcaagaa tttcgtcgcc
     1561 cgggtgtggg agtggaagca ggagtcgggc tcgaccatca cgcgccagat gcgccgcatg
     1621 ggcgactccg tgagctggca gcgcgagtac ttcacgatgg acgagaagct ctcgcgcacc
     1681 gtgaccgaga ccttcgtgcg cctgtacgag gaaggcctga tctaccgcgg cagccgcctc
     1741 gtgcactggg acccgcagct gaagtcggcg gtctccgacc tcgaggtgaa cagcgaggag
     1801 gaggacggct tcctgtggct gatccgctat ccgctggccg acggctccgg cagcctcacg
     1861 gtggcgacga cgcggcccga gaccatgctg ggcgacaccg cgctgatggt caatcccgag
     1921 gacgagcgct acgccgcgct ggtcggcaag atggtggtgc tgccgctggt gggccgcgag
     1981 atcccggtca tcgccgacgc ctatgtcgac cgcgagttcg gcaccggcgt cgtgaaggtc
     2041 acgccggccc acgacgagaa cgactacgcg gtcgggcagc gccacggcct gcccatcatc
     2101 ggcgtgctga cgctggacgc gaagatcaac gagaacgcgc ccgagcagta ccgcggcctc
     2161 gaccgcttcg tcgcgcgcaa gcgcgtggtg gcggacctgg aggcggcggg gctgctggtc
     2221 gaggtgcgca agcacaagct gatggtgccg cgctgcgagc gcaccggcca ggtgatcgag
     2281 ccgatgctga ccaaccagtg gtacgtcgcc gtgaccaagc cgggcgcgga cggcagctcg
     2341 atcgcggaca aggcgatccg ggcggtgtcc tcgggcgagg tgaagttcta tcccgagcag
     2401 tgggtcaaca cctacaacca ctggatgaac gacctgcagg actggtgcat ctcgcgccag
     2461 ctgtggtggg gccaccagat cccggcctgg tacgccgacg acggccgcat cttcgttgcc
     2521 cgcgacgacg ccgaggcgcg cgcgaaggcc gaggccgccg gctacaccgg cccgctcgtg
     2581 cgcgacgagg acgtgctcga cacgtggtac tcgtcggcgc tcgtgccgtt ctccacgctc
     2641 ggctggccgg agaagacgct ggagcaggag ctcttcctgc cgtcgtcggt gctcgtgacc
     2701 ggctacgaca tcatcttctt ctgggtcgcc cggatgatca tgatgacgac gcacttcacc
     2761 ggcaaggtgc cgttccgcga cgtctacatc cacggcctgg tgcgcgactc gcagggccag
     2821 aagatgtcca agtcggaagg caacgtgctc gatccggtcg acctgatcga cggcatcgcg
     2881 ctcgagccgc tgctggacaa gcgcgtcgtc ggcctgcgcc ggcccgagac cgcgcccaag
     2941 gtgcgcgagg cgacgaagaa ggagttcccg gaaggcatcc cgggctatgg cgccgacgcg
     3001 ctgcgcttca cgttcgcggc gctcgcgagc ctcggccgca acatcaactt cgactccaag
     3061 cgctgcgagg gctaccgcaa cttctgcaac aagctctgga acgccaccaa gttcgtgctg
     3121 atgaactgcg agggcgagga ctgcggcatc ctggaaggcg atccgaagca gtgcccgccg
     3181 ggccacaacg ccttcagcca ggccgaccgc tggatcacga gcgagctgca gcgcaccgaa
     3241 gccgcggtgg cccagggctt cgcggactac cggctcgaca acgtcgccaa cgcgatctac
     3301 cagttcgtgt ggacggagtt ctgcgactgg tacctggaga tcgccaaggc ccagctcgcc
     3361 gacgccaagg ccgcgggcga catcgcgcgc cagcgcggca cgcgccgcac gctggtgcgc
     3421 acgctggaga cggtgctgcg cctgctgcac ccgatcacgc ccttcgtgac cgcggagctg
     3481 tgggagaccg tcgccccgat cgcgggccgc gcgccggcgg cgggccagac catcgcgacc
     3541 gcgtcctacc ccgtcgcgca gctcgagcgc atcgacgagg cggcctgcgc ctgggtcgcg
     3601 caactcaagg cggtggtcgg cgcatgccgc agcgtgcgca agcagatgaa cctgtccgac
     3661 gccgagcgca tgccgctgct gacgcatggc gacgccgcgt tcgtcgaggc tgccgcacca
     3721 ctgctgaagg cgctcgccaa ggtcagcgag gtgcgcgtga tcgccgacga ggcggcgttc
     3781 accgagcaga cgaagctcgc ccccgtcgcg gtcgagggcg ccctgcgcct cgcgctgcac
     3841 gtcgagatcg acgtcgaggc cgagcgcgcg cgcctggcca aggaggtcgc gcgactggac
     3901 ggcgagatcg tcaaggccaa cgccaagctg gccaacgaga acttcgtcgc ccgcgccaag
     3961 cccgaggtcg tggagcagga gcgcgcgcgg ctcgcgagct tcgtcgagac gctggcgcgc
     4021 gtgaaggcgc aactggcgca gttgggcggc tgaggcgcgc gggcgcgcgc agcctcgcgc
     4081 gcgcacgcct cacgcgcccg cgtccgcgaa ggttctcagc cctcgagcgg cacgttcgcg
     4141 agcatcacct gcagcgccac cagcgtctgc ccgtgcaggc gctccaggcg cgctgccttg
     4201 gcttcgtcga cgacgacgtg gccgttggcg tcgtgcgcga agggctggcc gaggatcgcg
     4261 gccatgtcga gcgggtagaa gctcatgtcg ccgtacgggc gcttcccgtc gaccgcaggc
     4321 atcggccaga gctcgggcgc ttcgatcccg aggatctcgc gcgcgtcctg ctcgtcgacc
     4381 ggacgccagt tggcggcgcg cagcagggcg aggtgctcga cgctcaggtc gaagcagcca
     4441 tcggcctcga cgcccagggt gccggcatgc gcgccaagcc aggcgagcgt ctcgggcggc
     4501 acggcgtagc ggccgggcgc gcaggcgacg ccgccagcga gcaggcccgg caggagccgc
     4561 ccggtctcga ccagccggcg cagcgcgagg cgctcgtccg tcgtctgcaa caggcgggcg
     4621 gcgaggcccg cgacggggcc gtcgccccgc aagggacgca ccgcgtcgat gtcaggcgcg
     4681 ccgctctcga ccggcgacca gcccacgcga agcccgcgca tgatggcgcg gtgctcgcgc
     4741 agcggcgtcg gcgcgggcgc ggcgaagacc tcggcgatga cctcgcggcg cagcgccgct
     4801 gccgacgtct cacgcgacac cgcgcatcct cctgccgcga gcgcgaacat cgaggcgacg
     4861 acggcgcgcc ggctggccat ggctcatcgc cgccgcagga agtggatgta ctccgccgcc
     4921 acctgctgct gctcgaccag ctcgttgccg gtctggcgcg cgaaggcctg gaagtcgcgg
     4981 gtggagctgg ggtcggtcgc gacgaccttg aggatctcgc ccgattgcat gtccgccagc
     5041 gccttcttcg ccttcaggat gggcagcggg cagttcaggc cgcgggtatc gatttccttg
     5101 ttggcatcca tgggcgggac ctcgtgaagg cggccattat cccctgccgt ccgcgggcgc
     5161 gctgcccggg tcgacggcgt cgcgcgactg cgcctcgcgg gtcgcccagt ccatgaagcg
     5221 gggctcgtag ccgcgcgcgc gcagccactc ggccatcgcg tagccggtgc ccgccggcca
     5281 gcagacgatg tcgcccggtt cgaacaactt gtactcggcg agctccggcg acagggcgat
     5341 ctcgccgtgc gcgaccacgt ggtacgcgac gatcacctgg ttcatgcgct ggaagtccca
     5401 gacgccgacc agcttcactg attcggccac gaggttcgtc tcctcgcgca cctcgcgcgc
     5461 gatgccgtcc tcgggcgtct cgcccgcctc catgaaaccg gtgatgagcg cgaaccggcg
     5521 ccccggccac gcggcgttgc gcgcgagcag gacgcggccg ccgcggtcgc tgcactcgac
     5581 gatggccgcg agcaccggcg tcgggttgtt ccagtgcgtg aagccgcagt tggcgcagcg
     5641 cagccgctcc ttcgggccgc cgtcctcggg cagcgtgatc ggcgccagcg cgtgcgcgca
     5701 ctgcgggcag aacttccagt cgacgccgct catggacgcg cgctcaggcc gggaacacgc
     5761 cggtggacag gtagcggtcg ccgcggtcgc agacgacgaa cacgatggtc gcgttctcga
     5821 gccgctgcgc gacctgcagc gcggcccagc aggcccccgc tgccgagatg ccgccgaaca
     5881 gcccttcctc gcgcgcgagg cggcgcgcca tctcctcggc atcgtcctgc gtgacctgga
     5941 tcgtctcgtc cacgcgcgag ggttcgtaga tcttcggctg gtacgcctcg ggccacttgc
     6001 ggatgcccgg gatgcgcgag ccttccgccg gctgcgcgcc gaggatgcgg atcgccgggt
     6061 tcttctcctt caggaagcgc gagacgcccg tgatcgtgcc ggtcgtgccc atggcgctca
     6121 cgaagtgcgt gacctggccc tcggtgtcgg cccagatctc cgggcccgtg gtctcgtagt
     6181 ggatgcgcgg gttgtcggga ttggcgaact ggtcgagcac cagccccttg ccgtcggcct
     6241 gcatctgcag cgcgaggtcg cgcgcgtatt ccatgccgcc cgacttcggc gtgaggacga
     6301 gctccgcgcc atacgccttc atcgtctgca cgcgctcgac cgacaggtcc tccggcatca
     6361 ccagcaccat gcggtagccc ttgatcgccg ccgccatcgc cagcgcgatg ccggtattgc
     6421 cggaggtcgc ctcgatcagc gtcgcgccgg gctggatctg gccgcgctcc tcggcgcggc
     6481 ggatcatcga catcgccggc cggtccttca ccgagcccgc cgggttgttg ccctcgagct
     6541 tgcccaggat gacgttgccg cgcgcggcga cctccgcggg cacgacgcgc tgcaggcgca
     6601 cgagcggcgt gcggccgatg gcgtcttcga gggtgaggta cttcggcatg gcggctcgca
     6661 gcgggatcgt ggtcggggaa gcccgcattg tggcagtgcc gtccaacccg cgcgttcgtc
     6721 atgtgataat ccgccctccc cgcgcatccc ctgcgcgacg ggcctcggtg gcgtaatcgg
     6781 tagccgcgac ggactcaaaa tccgttctcg aaaggggtgt cggttcgagt ccgacccgag
     6841 gcaccaggcg accaattcgt cttcagaatc aacggcttgc ggcaactacg ctggcaagcc
     6901 cttttgtttt ggcgacccgc ctccagtttg gtttcggctg tccgttgcca gtctggtgcc
     6961 agtttgagcc ggatgccgtt ggacggtcag cggccacgcg gctctctagc cgaaactcgc
     7021 ggccggccag tttgcggctt gccgtgcgcg agcgccggaa gcgccgctga aatcgttcga
     7081 tccgcgcgtt ccgagagcat cgggtcgatc cgccgcaact ccgcgatcag ctctgcctgc
     7141 gtcaagccga agacgtagcg gccaccctcg tccgcaacga ggccccgctc cgaagcgagc
     7201 gcatccactg cggcacgaat ccgctggaac agccggccac ccaccgtgcg ctggcgctct
     7261 tttggcgaca accgccgtat gacgttgggc acgcggcccc aacgggtgcg cacacccttg
     7321 gcgacctcga acacttcctg cgccacgacc ttccagtgat gcacgaagtt gacctggaca
     7381 cccaactgaa ggctcgcttc gtactcgaag gccagcggga tgtgccaggt ggactccgag
     7441 ggaatcggcg cgcagcggat cggcacggcc gaatctccgt cacgccagca aagccaagcc
     7501 acctgacggc cgtgctgcca gcgggcgcac ctccaggcgt tgtgagcctc ccagaacctg
     7561 cgatggacgg gaagtttgaa ctgcgtcgcc caacgaatcc acccgtggac gttcagcgga
     7621 tacagatact ttgccgcgtc agtgtccgcg agcggaagca gcatcggccg aatggacatg
     7681 tctacgactg gagctcgtcc cgcaaacata accgaatgcg cgacgagcca gcggttgtac
     7741 ttcttgcgta tgcggtcgag caccttacgc ctcgcaggtg acgggcttaa agctttgctc
     7801 tcgggccgtc caacgaaccg gtcatcactc gactcgcccc attcaaacga atcgtccgtc
     7861 tccatccact cgcgctcttg ctcatcggcc tgcgccgcct cgtccgcaag catctcctcg
     7921 aaggacttgc gatcgctgct cggctgatca catggcgcct cccccgtgga gagcatccat
     7981 atggcatcga gcgcgctcca gttctgttcg tcgaagaacg ccacgtcctg tggttcaaac
     8041 gtgctcaaga cgtcttcgag ctgcggcccc tcgtagaacg gcgcgccctc gggccagatg
     8101 gcgtttcgta cctccatcgc ctccggcgat cggtctggct ctgaaaggac gatctcggtc
     8161 agtctcttga tggcgttcac atcctgcata ttcctgcata ttaagacaag ccgatccttc
     8221 gttccacgac gtccgtcgac gttcgacaac cgaaaggaat cgcatgatca gtaccaacca
     8281 atgggcccag gcccacgccg cggccaccgg cactcgcgtg acggaggtct acctcccgag
     8341 cgaaggcttc atcgacgagg aagtgttctg cgcgctcttg accgtgaagc ggtcgacgtt
     8401 ctggcaaggc atcaaggacg gccggtatcc gaagccggtg aaggtctccg cgaggaaaaa
     8461 cggctggccg gtggccaaag tgcgcgctct gttcgcgtcc ctggccggtc aagcggacgc
     8521 catccacggc gcctgaacgg aggaatcgca catggcaaac aagcaacacg gcgaaagcga
     8581 gatcctcgca gcgctgcggg agcggtatgg gctgcgcagg accaaggacg gtctcgtcgc
     8641 agaagtcgac agtgacgtgg tcaagctgtc tgatcgcttt catgtcggcg gcatcgcgcg
     8701 cgcagcagac ggacgggtgc tcggggtcat cctgcaggcg ggcaacggcg gtgcgcgccg
     8761 cctctgcatc gcaccgtatc aggccatccg cgagccgaag cagctcgcca gctacttcga
     8821 gtacctcggc ctggtgccgc cggccgaact gcgcgagctg aaaatactgg cgacctacat
     8881 cacggcgctg gccggcctgc ggtcccgggt cgtcctcgcg cgcgaaggta tccaccaagt
     8941 gtcctgcgac gggaagcgca agcccgtggc cgtcatcggc cgagaggtga tcggcgcccc
     9001 cgcagacgaa ttcctggcgc tgccctcttc ggtgtcgtgc tactcctcgc agggcgatct
     9061 ggccggatgg caacgcgggt tcaagccgct gctcctcgcg aatcccctca tgacgctggc
     9121 cgcgtgcttc gcgctggctg ttccgctggg aagcctgctc ggcttcccgc tcaccgggct
     9181 cttgctggca ggcgcctcca gcaccggcaa gacgacgctg gcgcgcttcg tcacttccac
     9241 cttcagtgca ccgcgcgagc ctcaccagtg gtcgggcacc gccaacggca tcgaagccct
     9301 cgccgccctg cacgccgacc tgccgctctc gctggacgaa ctcggcgcgg gcgacatccg
     9361 ccagacgctc gcgacgatct accgcgtcag cggcggccgc gccaaggctc gggccaccat
     9421 gaccgggcaa ttgcagcaag gcgagccgct gcgctgcgca ctcttcgcca caggggaggc
     9481 gaccttgaac caacaggcga acgcggcagg caccgacgtg cgggcgggac atgacgtcag
     9541 gatgcccacc atctgggtcg acgaagcgca cggcgtctac acggacatcg ggaaggccgc
     9601 tgacggggcc gcgtacgccg agcgcgtcgc catcgcgatg caggaaaact acggcgtgct
     9661 gttgcccgcc ttcgtgggag ccgtcgttgg agacgtcgat ggcgcccgcg agacggccga
     9721 gggcatgcgc accgccatcc tgacgcaggt cgccggcgtc gacgtcctcc acatgaccgg
     9781 ggtcgagcgc cgggtgctta cggcgttcac gaactgggcg atcgccggcg aactggcagt
     9841 ggcctatggc gctctgccgc tgaaggccgg ggagccggct gccgccattg cttacgtgtt
     9901 cgcgaagtgg ctcgcacgtt ggcgggcact cgcgcaaggc ccctctttgg ctccgctgcg
     9961 caacctgagt gacttcttca agcgaaatca ccaccgcttc gcgccgatca aggactggca
    10021 ggacagcgcc tccgcatccg gacgcctggg ctatcgccgc gcaaccgcca gccacggcga
    10081 ccacttcctg gtcttccgcg atgtgttcga gaaggacgcc gcgcaaaagc acggcgtgga
    10141 tgcgtgcgtc aacgcgctgc gcgacgccgg cttgctgatc gccggcaaga agggccgaac
    10201 cctcctcgtc cgcatgccgc gcagcgagga cggtgaacgc atggccctct acgcgattcg
    10261 cgcgaacgtg atgttcgata ccgaggagtg atgtcggaag gtcagttccg acagatgcga
    10321 atcacgaggc ggcgagcggg tgtcggagtg tcagttccga caattcggca tgacgcgatt
    10381 tcgaggtctg tgcatatctg cacagaccac ttccgcacgc ggcggcagcg gcacgtgtga
    10441 actgtatcca cccacccttt ctacctacct ctctgccacc cgcacatcca cttttgccat
    10501 ttgcggatgc gccactgcgc caaagacctg cgcgaacgtg cccgtcactt cggtaaatgg
    10561 cgcagtgtgc gcgtgcggcg catcttcacc acaaattcac cttcgaggta tccatgatcg
    10621 actactacga catcgaactg aatcagattc ggctcggccc gaaagaatgg cgtcgcattc
    10681 ccgggcacga aaagcccacg cgaccgccca atcgcatgca cgtcaagctg aaggagaagg
    10741 ggtgggcggc cacgcaagaa gccctgattc cccacgattc gctccgtctc ctcgtctgtc
    10801 cgcaaaaggt gttggggcac tggaactgcc acggctccga cgaccttcgc caagtggttc
    10861 tcgacacggc cccggctatg cttcgcgcga tgggcttgga ggtgacgcag gaaattatga
    10921 gtgacctggt gctgggaaag tacggcatcc gaaaggtgca tatcgcccac cagttctggc
    10981 tcgacgaccg ctgcattcgc gacttcctgc gagccgtacg tcgtcgcctg aacgaatcgc
    11041 acgagccggt gcccgtgttc cgcggcgacg gcttcatgat cggcaaccgc tcgcgcacgg
    11101 tcgaatacgt cttctacgtc aagcttcggg aattcctgaa gtacggcctc cccttctacc
    11161 gcgagcgtct cgctcacgag cgagcaaaag gccgtcctta cggcgcccga acgggcctgg
    11221 agcgcgacct tcagcgcatc gcagccgccg caggcccgcg tctcgagatg cggttggggg
    11281 atcactactt ccgcaagaac cggaatttcg ctcgcggcac tggctgggcg gcggccacgg
    11341 ccgatggcat ctaccaacag cacctgcgca agctggaact gccgccggtc gtcgaggctg
    11401 cccctgctcg tgtgcgcgcg cgtcagcgcc tgaaaaccgc gctgaagaca tacctcctct
    11461 gggccgatgg acacccgctg aacgatatcg gcaccaacgt caaaaccgtt gccagccatc
    11521 gcaggatcat tctccaggaa ctgggctttg acatccgcac gccggcgagc gcggtgttcg
    11581 gcgacaacct gacagtcgac gcgcgttcgg tctttgactg gaagaaccgg gtgcaggcgg
    11641 cgaaggtgga cgtggacgac ctgtactggc tcggagaccc gtatgaaccg gcctactagc
    11701 ggacgcgtcc gccgcccccc ccggctgaac tagggatggt ctgcaaaacg tgacacggag
    11761 cgctgcgcag ccctgttgat gccacacgta tcgtcaggtc gcatgtctgt cggcctcgat
    11821 caggcggcag cgcaggcgct tggccctgtc gcagggcgca tttcaggctc
//
LOCUS       DASVLN010000782         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_255727, whole genome shotgun sequence.
ACCESSION   DASVLN010000782 DASVLN010000000
VERSION     DASVLN010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255727"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /gene="pcnB"
                     /locus_tag="VF453_18535"
     CDS             complement(<1..1422)
                     /gene="pcnB"
                     /locus_tag="VF453_18535"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX7689709.1"
                     /translation="MIRKFIDRLLGKGPAANAPAATEASTGPVLGERVEFGVDQHRID
                     PKLVNDQAIKVVRTLKEAGHEAYIVGGAVRDLLVGLEPKDFDVATDATPEQVKALFRR
                     AFIVGRRFRIVHVVYGRGRDHEKIEVSTFRAYVDNADAAAVPGNEKTSKEELAGRSHA
                     VDASGRVLRDNVWGPQVEDAARRDITVNAMYYDPQAQVVVDYHGGMADSRAKLLRMIG
                     DPEVRYREDPVRIIRVLRFAAKMGYRIEPRTEAPIRRMKALLANVPASRMFDEMIKLL
                     QTGHALASIEVIRKYGLDRGVFPILDAHFEAIAGNPAREAFVNRALADTDRRVNEGKP
                     VAPSFLLACMFWHEVSQRWQAIRDQGEGPTPALQAAIDATFDARIGDISGRGKIAADM
                     RDIWMMQPRFERRTGTAPVSLVQQSRFRAAFDFMRLRAEAGEVDGELVEWWEKFSLGD
                     DDQRRALLEAARKAQPARREKAAP"
     gene            complement(1419..2111)
                     /locus_tag="VF453_18540"
     CDS             complement(1419..2111)
                     /locus_tag="VF453_18540"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7689710.1"
                     /translation="MTPPSRDLTLFDLDGTLISGDSDHSFGEFMVQVGWADGDAWRAR
                     NDEFYKTYLEARLDVHAYVDFATSVWRRRSAGEQAEISARFMREVIAPMITPRALELV
                     REHQRRGDLVAIVTATNEFVTRPIAEAFGVPELLAIELERDAAGAVTGAIRGVPTYQA
                     GKVTRVEQWLAAHPAGPLSWSDFGRISVYSDSTNDLPLLERATDPVATNPSAALAKVA
                     AERGWRLLRLFE"
     gene            complement(2108..>2412)
                     /locus_tag="VF453_18545"
     CDS             complement(2108..>2412)
                     /locus_tag="VF453_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEX7689711.1"
                     /translation="RVPPVDLPVREDLRTRLGWGGVHAVRPLAEGDMRVALREEARRR
                     GIVLADEVLDYLLTRFARDLKSLMHLLHRLDGFALAEKRAVTVPLLRRMLNEESAA"
BASE COUNT          319 a          838 c          901 g          354 t
ORIGIN      
        1 cggggcggcc ttctcgcgcc gcgccggctg cgccttgcgc gcggcctcga gcagcgcgcg
       61 gcgctggtcg tcgtcgccca acgagaactt ctcccaccac tcgaccagct cgccgtcgac
      121 ctcgcccgcc tcggcgcgca ggcgcatgaa gtcgaacgcg gcgcggaagc gcgactgctg
      181 caccagggac accggcgcgg tgcccgtgcg gcgctcgaag cgcggctgca tcatccagat
      241 gtcgcgcatg tcggcggcga tcttgccgcg gccggagatg tcgccgatgc gggcgtcgaa
      301 ggtcgcgtcg atggccgcct gcagcgcggg cgtcggcccc tcgccctggt cgcggatcgc
      361 ctgccagcgc tgcgagacct cgtgccagaa catgcacgcg agcaggaagc tcggcgccac
      421 cggcttgccc tcgttgacgc ggcggtcggt gtcggcgagc gcgcggttga cgaaggcctc
      481 gcgcgccggg tttccggcga tggcctcgaa gtgcgcgtcg aggatcggga acacgccgcg
      541 gtccaggccg tacttgcgaa tgacctcgat gctggccagc gcgtggccgg tctgcagcag
      601 cttgatcatc tcgtcgaaca tgcgcgaggc cggcacgttg gcgagcagcg ccttcatcct
      661 gcggatcggc gcctcggtgc gcggctcgat gcggtagccc atcttcgcgg cgaagcgcag
      721 cacgcggatg atgcgcaccg gatcctcgcg gtagcgcacc tcggggtcgc cgatcatgcg
      781 cagcagcttg gcgcgcgagt cggccatgcc cccgtggtag tcgacgacga cctgcgcctg
      841 cgggtcgtag tacatcgcgt tgacggtgat gtcgcggcgc gcggcgtcct cgacctgcgg
      901 gccccagacg ttgtcgcgca agacgcggcc gctcgcgtcg acggcgtggc tgcggccggc
      961 gagctcctcc ttcgaggtct tctcgttgcc cggcacggcg gcggcgtcgg cgttgtcgac
     1021 gtaggcgcgg aaggtcgaga cctcgatctt ctcgtggtcg cgcccgcggc cgtagacgac
     1081 gtggacgatg cggaagcgcc ggccgacgat gaaggcgcgc cggaacagcg ccttgacctg
     1141 ctccggcgtc gcgtcggtgg cgacgtcgaa gtccttcggc tcgaggccca ccagcaggtc
     1201 gcgcacggcg ccgccgacga tgtaggcctc gtggcccgcc tccttgagcg tgcgcacgac
     1261 cttgatggcc tggtcgttga cgagcttggg gtcgatgcgg tgctggtcga cgccgaactc
     1321 gacgcgctcg ccgagcaccg gcccggtgga cgcctcggtc gcggccgggg cgttcgccgc
     1381 ggggcccttg cccagcagcc ggtcgatgaa tttgcggatc attcgaagag cctcaggaga
     1441 cgccagccgc gctcggccgc caccttcgcg agcgcggccg acgggttggt cgcgacggga
     1501 tcggtggccc gctcgagcag cgggaggtcg ttggtggaat cactgtaaac gctgatgcgg
     1561 ccgaagtcgg accacgacaa ggggcccgcc gggtgcgccg cgagccattg ctccacgcgc
     1621 gtgaccttgc cggcctggta ggtcggcacg ccgcggatcg cgcccgtgac ggcgccggcg
     1681 gcgtcgcgct cgagctcgat ggccagcagc tcgggcacgc cgaaggcctc ggcgatcggg
     1741 cgcgtgacga actcgttggt ggcggtgacg atggccacga ggtcgccgcg gcgctggtgc
     1801 tcgcgcacga gctcgagcgc ccgcggcgtg atcatcggcg cgatgacctc gcgcatgaag
     1861 cgcgcgctga tctcggcctg ctcgccggcg ctgcggcggc gccagaccga ggtggcgaag
     1921 tcgacgtagg cgtgcacgtc cagccgcgcc tcgagatagg tcttgtagaa ctcgtcgttg
     1981 cgcgcgcgcc aggcgtcccc gtcggcccag ccgacctgca ccatgaactc gccgaacgag
     2041 tggtcggagt cgccggagat cagcgtgccg tcgaggtcga acagcgtgag gtcgcgggaa
     2101 ggcggggtca tgcggcggat tcttcgttga gcatgcgcct gagcagcggc acggtgacgg
     2161 cgcgcttctc ggcgagcgcg aagccgtcga ggcggtgcag caggtgcatc aggctcttca
     2221 ggtcgcgcgc gaagcgggtg agcaggtagt cgagcacctc gtcggccagc acgatgccgc
     2281 ggcgccgcgc ctcctcgcgc agcgccacgc gcatgtcgcc ctccgcgagc ggccgcacgg
     2341 cgtgcacccc gccccagccc aggcgggtgc gcaggtcttc ccgcaccggc aggtcgaccg
     2401 gcggcacgcg ac
//
LOCUS       DASVLN010000783         1915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_255756, whole genome shotgun sequence.
ACCESSION   DASVLN010000783 DASVLN010000000
VERSION     DASVLN010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255756"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VF453_18550"
     CDS             <1..247
                     /locus_tag="VF453_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7689712.1"
                     /translation="VPLPDGPRCHAVAACVGRAAGRLRAQVGDGLVPVDSALGRHDDP
                     ARQLRFEADRQVVFERMNHMALLARPEVTQALMRWLC"
     gene            384..890
                     /locus_tag="VF453_18555"
     CDS             384..890
                     /locus_tag="VF453_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689713.1"
                     /translation="MPNLAVALGLALCTAGCSSLLSEGSATAAGVAGTAVAGAVTKNA
                     TVTAGIGLGVLAAAQAGVKAVERDYHGHQQDLIAEIAGELAVGQVAPWRSRHAIRLEP
                     DEAGRVTVSRVISAMGLACKEIVFSVDHVEDAKTLSSFYVATVCRDSLKWRWASAEPA
                     TARWGSLQ"
     gene            887..1393
                     /locus_tag="VF453_18560"
     CDS             887..1393
                     /locus_tag="VF453_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689714.1"
                     /translation="MTRDARRWTPAVAALASALAGCSSIGGFTGAVAGAATGTFSSNP
                     TVGVAVGVSVKAATDAAMNRVFRGMQSDEQDRIAELAGQLDVGDRRSWQVHHLFSYGD
                     EAGELQVLGSIDNALASCKEVLFSVAGGKRDAPTRQWFVTQTCRQSDGRWRWAAAEPA
                     TGRWGSLQ"
     gene            complement(1487..>1915)
                     /locus_tag="VF453_18565"
     CDS             complement(1487..>1915)
                     /locus_tag="VF453_18565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689715.1"
                     /translation="DTAASASPEGAAADARSSFDPRRLDSVRANLDAALAKGASCSAD
                     SECRTVAVGGKACGGPTGYRAYSAQGADPKTIESLAADERQLSLQQARAEGRVSPCFM
                     LADPGAHCEAHKCVTGPSAGLPGNGRLVPAPPSASASAVR"
BASE COUNT          240 a          686 c          744 g          245 t
ORIGIN      
        1 ggtcccgctg cccgacggcc cgcgctgcca tgcggtcgcg gcgtgcgtcg gccgcgcggc
       61 cggtcgcctg cgggcgcagg tgggtgatgg actggtgccg gtcgacagcg cgctcggccg
      121 gcatgacgat cccgcgcggc aactgcggtt cgaggccgat cggcaggtgg tcttcgagcg
      181 catgaaccac atggcgctgc tcgcgcggcc cgaggtgacg caggcgttga tgcgctggct
      241 ctgctgaccg cccgggtcgc gcgcgggctc gcgtcggcgc cctcgccaac cggagcaggc
      301 gcgaggaatg ccggtggcgg ccggtcccac ctctgccgcc gcaacggaac atgcctgccc
      361 gccccgcctg tgcgcatccg gccttgccga acctcgccgt cgcgctcggt ctcgcgctgt
      421 gcacggccgg gtgcagttcc ctgttgagcg aaggctcggc cacggccgcc ggcgtcgcgg
      481 gcacggccgt ggcgggggcg gtgacgaaga acgccacggt gaccgccggc atcggcctcg
      541 gggtgctcgc cgcggcgcag gcgggcgtca aggccgtcga gcgcgactac cacggccacc
      601 agcaggatct catcgcggaa atcgccggcg agctagccgt cgggcaggtc gcgccctggc
      661 gcagccggca cgcgatccgg ctcgagcccg acgaggcggg gcgggtcacg gtcagccgcg
      721 tcatcagcgc gatgggcctc gcctgcaagg agatcgtctt ctccgtcgac cacgtcgagg
      781 acgcgaagac gctgagctcg ttctacgtcg ccaccgtctg cagggactcg ctcaagtggc
      841 gatgggcgtc cgcggagccg gcgaccgcgc gctggggcag cctgcaatga cccgggacgc
      901 gcggcggtgg acgccggcgg tggcggcgct cgcctcggcg ctcgcgggct gcagctcgat
      961 cggcggcttc accggggcgg tggcaggcgc cgcgaccggc acgttcagca gcaatcccac
     1021 ggtgggcgtc gccgtcggcg tctcggtgaa ggcggcgacg gatgctgcga tgaaccgggt
     1081 gtttcgcggc atgcagtccg acgagcagga ccgcatcgcg gagctcgccg gccagctgga
     1141 cgtcggcgac cgccggtcct ggcaggtcca tcacctgttc tcgtacgggg acgaagccgg
     1201 cgagctgcag gtcctcggca gcatcgacaa cgcgctcgcc agctgcaagg aagtcctgtt
     1261 ctcggtggcg ggcgggaagc gggacgcacc gacccggcaa tggttcgtca cgcagacctg
     1321 ccggcagtcg gacggccgct ggcgctgggc ggcggccgag ccggcgaccg ggcgatgggg
     1381 ctcgctccaa tgaggcgccc gcccgccggc aagcgccgga cgaagagagg ccgcgcaccc
     1441 ggaggtggcg gcctcgccgt cgagatgccg atgaagggcg gccgcgtcag cgcaccgccg
     1501 acgcgctggc cgacggcggt gccggcacca ggcgtccgtt gcccggcagc ccggccgaag
     1561 ggcccgtgac gcacttgtgc gcctcgcagt gcgcgcccgg atccgcgagc atgaagcagg
     1621 gcgatacgcg gccttccgcg cgggcctgtt gcaggctcag ctggcgttcg tccgcggcga
     1681 gcgattcgat cgtcttcggg tccgcgccct gcgccgagta cgcgcggtag cccgtcgggc
     1741 cgccgcaggc cttgccgccg acggcgacgg tgcggcattc cgagtccgcg ctgcacgacg
     1801 cgcccttggc gagcgcggcg tccaggttgg cccgcaccga gtcgaggcga cgcgggtcga
     1861 aggacgacct cgcgtcggcg gcggccccct ccggcgaggc cgaggccgcc gtgtc
//
LOCUS       DASVLN010000784         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_525867, whole genome shotgun sequence.
ACCESSION   DASVLN010000784 DASVLN010000000
VERSION     DASVLN010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525867"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1517
                     /locus_tag="VF453_18570"
     CDS             <1..>1517
                     /locus_tag="VF453_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-1,6-glucosidase domain-containing protein"
                     /protein_id="HEX7689716.1"
                     /translation="AAAARIAATDCFNWGYDPLHYGAPEGGYATDADDGAVRVREFRA
                     MVEALHAAGLRVGMDVVYNHTPASGQAELSELDRTVPGYYQRLDKDGAVERSTCCDDT
                     APEHAMMGRLVVDTALRFATQYRIDSFRFDLMGHLPRALLETLQHRLREATGHDVPLI
                     GEGWNFGEVADGARFVQASQLSLGGSGIGTFNDRLRDALRGGSATDGTHVLADQGWLN
                     GESFDPNGHGHGHATRDDLAAAADLVRAGLAGSLRDFAFTTRDGTRRTLHELAYGAQP
                     AGYASAPAEAVNYVENHDNLTLWDNDTLKLPRSTSREDRARVQVLGAAVVAFSQGVAY
                     FHAGVDTLRSKSFDGNSFDSGDWFNRLDWTYADNGFGSGLPLRSENGARWPVLAPFLA
                     DASLQPAPADIAWTRDAFRDLLRIRASSALFHLASADEVRARLSFPPASTGPRADPAM
                     IVERLDGRGLPGARFGAVLLFLNADRRAHAIRLPEEAGRAWTLHPVQRSPRAADR"
BASE COUNT          199 a          591 c          531 g          196 t
ORIGIN      
        1 ggccgcggcg gcgcgcatcg ccgcgaccga ctgcttcaac tggggctacg acccgctgca
       61 ctacggcgcg cccgaaggcg gctacgcgac cgacgccgac gacggcgccg tgcgcgtgcg
      121 cgagttccgt gcgatggtcg aggcgctgca cgccgccggc ctgcgcgtcg gcatggacgt
      181 cgtctacaac cacacgccgg ccagcggcca ggcggagctg tccgagctgg accgcaccgt
      241 gccgggctat taccagcgcc tcgacaagga cggcgcggtc gaacgctcga cctgctgcga
      301 cgataccgcg cccgagcacg cgatgatggg ccggctggtg gtggacacgg cgctgcgctt
      361 cgcgacgcag taccgcatcg actcgttccg cttcgacctc atgggccacc tgccgcgcgc
      421 gctgctcgag acgctgcagc accgcctgcg cgaggccacc ggccacgacg tgccgctcat
      481 cggcgagggc tggaacttcg gcgaggtggc cgacggcgcg cgcttcgtgc aggcgtcgca
      541 gctgtcgctg ggcggcagcg gcatcggcac cttcaacgac cgcctgcgcg atgcgttgcg
      601 cggcggctcg gcgaccgacg gcacccatgt gctggcggac cagggctggc tcaacggcga
      661 gtccttcgat cccaacggcc acggccacgg ccacgcgacg cgcgacgacc tcgccgccgc
      721 cgccgacctc gtgcgcgcgg gcctggccgg ctcgctgcgc gacttcgcct tcacgacccg
      781 cgacggcacg cgccgcacgc tgcacgagct ggcgtacggc gcccagcccg cgggctacgc
      841 gagcgcgccg gccgaggcgg tcaactacgt cgagaaccac gacaacctga ccctgtggga
      901 caacgacacg ctcaagctgc cgcggtcgac ctcccgcgag gatcgtgcgc gcgttcaggt
      961 gctgggcgcc gcggtcgtcg cgttcagcca gggcgtcgcg tacttccatg cgggcgtcga
     1021 taccctgcgc agcaagtcct tcgacggcaa cagcttcgac tccggcgact ggttcaaccg
     1081 cctggactgg acctacgccg acaacggctt cggctccggg ctgcccctca ggagcgagaa
     1141 cggcgcgcgt tggccggtgc tcgcgccgtt cctggccgac gcgtcgctcc agccggcgcc
     1201 cgccgacatc gcctggacgc gcgacgcctt ccgcgacctg ctgcgcatcc gcgcgagcag
     1261 cgcgctgttc cacctcgcga gcgccgacga ggtgcgcgcg cgcctgagct tccccccggc
     1321 gagcaccggt cctcgggccg accccgcgat gatcgtcgag cgcctggacg gccgaggcct
     1381 gccgggcgcc cgcttcggcg cggtgctgct gttcctcaat gccgaccggc gcgcccacgc
     1441 gatccgcctg cccgaggagg ccggccgcgc gtggacgctg catcccgtgc agcgctcgcc
     1501 gcgggctgcc gaccggc
//
LOCUS       DASVLN010000785         2764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_310162, whole genome shotgun sequence.
ACCESSION   DASVLN010000785 DASVLN010000000
VERSION     DASVLN010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310162"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VF453_18575"
     CDS             complement(<1..412)
                     /locus_tag="VF453_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX7689717.1"
                     /translation="MSAPSPVPSHDDLVGVRSSIRRYDGRHLSHSHGYAQVMFALNGR
                     MELEIAGRTLVADASSGVVVPAGFEHGYCAEDSARMLVIDAPEASALARVRRFAVTMD
                     CLRRVRADDPAAALAAMLDAPEVLERRQIDLARLE"
     gene            496..1131
                     /locus_tag="VF453_18580"
     CDS             496..1131
                     /locus_tag="VF453_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX7689718.1"
                     /translation="MKDDPAASPLNDRIARRVRDLRAAAGLSLEALAAHCGISRSMIS
                     LVERGESSPTAVLLEKLASGLGVPLAALFDAPEPSPSPVARAADHPPWTDPHSGYVRR
                     NVSPAGVGSQVQIVDVHFPPGARVAYETGPREPVMHQQVWLIEGVIEITVGTDVHRLE
                     AGDCLAMILDRPIAFHNPGRATARYAVVLASMTHPLPFRRSHADPRPSLYP"
     gene            1100..1663
                     /locus_tag="VF453_18585"
     CDS             1100..1663
                     /locus_tag="VF453_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7689719.1"
                     /translation="MPTRDPASTPDGIAVRRLERVGAAELQALADVLVDCVEGGASVS
                     FMLPISQERALAFWRRVADGVHAGRRVLVVAEDAQGLCGTVQLDLDLPENQPHRGEVA
                     KMLVRRRARRRGVGEALMRAIEAEARVRGRTVLVLDTGDPTAARLYRRCGWIPVGTVP
                     GYALMPDGAPCDTTFYYRRLDPVASAG"
     gene            complement(1694..2641)
                     /locus_tag="VF453_18590"
     CDS             complement(1694..2641)
                     /locus_tag="VF453_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016348037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX7689720.1"
                     /translation="MPAQIAVAVLDDYQHAARRFADWSAVDARASVTVFHDHLAGIDA
                     VASRLEPFDVLCVMRERTPLPRRLLERLPRLKLVVSTGARNASIDLDACAERGIRVLH
                     TGYHSYPTIEMTWALILAASRHVVREAEAVRSGDWQQAVGTGLHGKTLGVLGLGRVGG
                     EVARVGAAFGMRLLAWSPNMTPERAAEHGATAVTKEQLLRESDVLTIHMVLSSKTRGL
                     VGAADLALMKRTALLVNTSRGPLVDEAALVDALRGKRIAGAALDVFDVEPLPGGHPLR
                     VLDNVLCTPHVGYVADDLYRIFFGDTVKALAAWLDERAA"
BASE COUNT          360 a         1036 c         1008 g          360 t
ORIGIN      
        1 cctcgagccg cgcgaggtcg atctggcggc gctccagcac ctcgggggcg tccagcatcg
       61 ccgccagcgc cgcggcagga tcgtcggcgc gcacgcggcg caggcagtcc atcgtcacgg
      121 cgaagcgccg cacgcgcgcg agggcggacg cctccggggc gtcgatcacc agcatgcggg
      181 cgctgtcctc ggcgcagtag ccgtgctcga agcccgccgg cacgaccacc cccgaggacg
      241 cgtcggccac gagcgtgcgg ccggcgatct cgagctccat gcgcccgttc agcgcgaaca
      301 tgacctgcgc gtagccgtgc gaatgactca ggtggcggcc gtcgtagcgg cggatgctcg
      361 aacgcacgcc gaccaggtcg tcgtgggaag gaacggggga cggcgcggac atcgcgcgag
      421 catagcgcga cggccggcgc cggatgtcga gcgcctcttg acccgtcttc caacatactg
      481 gacactaatt ccatcatgaa ggacgacccc gccgcctcgc ccctgaacga ccgcatcgcc
      541 cgccgggtgc gcgacctgcg cgccgcggcc gggctgtcgc tggaggcgct tgccgcccac
      601 tgcggcatca gccgctccat gatctcgctg gtcgagcgcg gcgagagcag cccgaccgcc
      661 gtgctgctcg agaagctcgc cagcggcctg ggcgtgccgc tggccgcgct gttcgacgcg
      721 cccgagccct cgcccagccc ggtcgcccgc gccgccgatc atccgccgtg gacggacccg
      781 cactccggct acgtgcggcg caacgtctcg cccgcgggcg tgggctcgca ggtgcagatc
      841 gtcgacgtgc acttcccccc gggcgcgcgc gtcgcgtacg agaccggccc gcgcgagccg
      901 gtgatgcacc agcaggtctg gctcatcgag ggcgtgatcg agatcaccgt cggcaccgac
      961 gtccaccgcc tcgaggccgg ggactgcctc gcgatgatcc tcgaccgccc gatcgccttc
     1021 cacaaccccg gccgcgccac cgcgcgctac gccgttgtgc tcgcgtcgat gacgcatccc
     1081 ctgcccttcc ggagatcgca tgccgacccg cgacccagcc tctacccctg acggcatcgc
     1141 cgtccgccgc ctcgagcgcg tcggcgccgc cgagctccag gcgctcgccg acgtgctggt
     1201 cgactgcgtc gagggcggcg cctcggtgag cttcatgctg ccgatctcgc aggagcgcgc
     1261 gctcgcgttc tggcgccgcg tcgccgacgg cgtgcacgcg ggccggcgcg tgctggtggt
     1321 ggccgaggac gcgcagggct tgtgcggcac ggtgcagctg gatctcgacc tgccggagaa
     1381 ccagccgcac cgcggcgagg tcgcgaagat gctcgtgcgc cggcgcgcgc gccgacgcgg
     1441 cgtgggcgag gcgctgatgc gcgcgatcga ggccgaggcc cgcgtgcgcg gccgcaccgt
     1501 gctcgtgctg gacaccggcg acccgaccgc cgcgcgcctg tatcgtcgct gcggctggat
     1561 ccccgtcggc acggtgccgg gctacgcgct catgcccgac ggcgcaccgt gcgacacgac
     1621 gttctactat cgccgcctcg atccggtcgc gtccgcgggc tgatacccgg cgggtatcac
     1681 cgcggcccgc ggctcaggcg gcgcgctcgt cgagccaggc cgcgagcgcc ttgacggtgt
     1741 cgccgaagaa gatgcggtac aggtcgtcgg cgacgtagcc gacgtgcggc gtgcacagca
     1801 cgttgtcgag cacgcgcagc ggatggccgc ccggcagcgg ctcgacgtcg aacacgtcca
     1861 gcgccgcgcc ggcgatgcgc ttgccgcgca gcgcgtcgac gagcgccgcc tcgtcgacca
     1921 gcggcccgcg cgaggtgttg accagcagcg cggtgcgctt catcagcgcg aggtcggccg
     1981 cgccgacgag gccgcgtgtc ttggagctca gcaccatgtg gatcgtgagg acgtcggact
     2041 cgcgcagcag ctgctccttc gtgaccgcgg tggcgccgtg ctccgcggcg cgctcgggcg
     2101 tcatgttcgg gctccatgcc agcagccgca tgccgaaggc cgccccgacc ctggcgacct
     2161 cgccgccgac gcgccccagc ccgagcacgc ccagcgtctt gccgtgcagg cccgtgccga
     2221 ccgcctgctg ccagtcgccc gagcgcaccg cctcggcctc gcgcacgacg tgccggctcg
     2281 cggcgaggat cagcgcccac gtcatctcga tggtgggata cgagtggtag cccgtgtgca
     2341 ggacacggat gccgcgctcg gcgcaggcgt ccaggtcgat cgacgcgttg cgcgcgccgg
     2401 tcgagacgac cagcttcagc cgcggcaggc gctcgagcag gcggcgcggc agcggcgtgc
     2461 gctcgcgcat cacgcacagc acgtcgaaag gttcgagccg ggacgcgacc gcgtcgatgc
     2521 cggcgaggtg gtcgtggaag acggtgacgg acgcgcgcgc gtcgacggcg gaccaatcgg
     2581 cgaatcggcg ggcggcgtgc tggtagtcgt cgaggacggc gacggcgatc tgggcaggca
     2641 tggacggcgg gctcgtggct gggatccacc gattctcttc gcggcgcccc gtccccggtg
     2701 acttccggga aaagccgcgg ccggccgcgc gctcagcggc gggcgtcgac gggtgcgtcc
     2761 gccg
//
LOCUS       DASVLN010000786         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_472055, whole genome shotgun sequence.
ACCESSION   DASVLN010000786 DASVLN010000000
VERSION     DASVLN010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472055"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..>1600)
                     /locus_tag="VF453_18595"
     CDS             complement(85..>1600)
                     /locus_tag="VF453_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689721.1"
                     /translation="YRIELASASETDALVACLAGLASTPRQRFDMHLAEAALWRTRNA
                     GERESRAYDAALREASQAGDKLLLGIVYGAIGWFHESRDTDRAQACFEESADFLRQAE
                     GEAPDAAEVASEYVAALVRLAWLYVLRNDPRSRAVLDLAESRRATAALETTAMLDQAW
                     GEYWRRADEPRLAISFRHKALHAFERLDDTRQILSTYNNLSLLYLEVKDYERALSHAH
                     RIVAMAGHAAVDPYLLSSALCNTGVAYFWLDRLDEAIETYGRSLAVARDADLRVIVNR
                     AHFNLAEAHFKRLRQRGDPLDEAEGDRHLAVVQAAPLAERDLWTAQAAPALKTEVLAT
                     AAVLPHERLWTQEAAAHFEEMREVQRQRAILALPGTPAEHVRAHLAIANAYLAISAKE
                     REAALALIGRHGLGGQFEAEIDLLGVTFSRGLTREKALMALWKQKCYGVLTEERISPV
                     LRQVMSSGSINKSGYAQLCQVGLATASKHLGTLAERGLLVQMGKGPSTRYVLPD"
BASE COUNT          204 a          553 c          624 g          219 t
ORIGIN      
        1 ggggccaggc cgcgagcgct cggccgctcc ggagcgagcg gcccagcccc ctcggggggc
       61 ggcgagcgca gcgagcgggg gccgtcaatc cggaagcacg tagcgggtgc tcgggccctt
      121 gcccatctgc accagcaagc cgcgctcggc gagcgtgccc aggtgcttgg acgcggtcgc
      181 gagcccgacc tggcacagct gcgcgtagcc gctcttgttg atcgagcccg aggacatgac
      241 ctggcgcagc accggcgaga tgcgctcctc ggtgagcacg ccgtagcact tctgcttcca
      301 cagggccatc agcgccttct cgcgcgtgag cccgcgcgag aacgtgacgc ccaggaggtc
      361 gatctccgcc tcgaactgcc cgcccaggcc gtggcggccg atcagggcga gcgcggcctc
      421 gcgctccttg gcggagatgg cgaggtaggc gttggcgatc gccaggtgcg cgcgcacgtg
      481 ctccgcgggc gtgccgggca gggcgaggat cgcgcgctgg cgctgcacct cgcgcatctc
      541 ctcgaagtgg gcggcggcct cctgcgtcca caggcgttcg tgcggcagga cggcggcggt
      601 ggcgagcacc tcggtcttca gcgccggcgc ggcctgcgcc gtccagaggt cgcgctcggc
      661 cagcggcgcg gcctgcacga cggcgaggtg gcggtcgccc tcggcctcgt cgagcgggtc
      721 gccgcgctgg cgcaggcgct tgaagtgggc ctcggcgagg ttgaagtgcg cgcggttgac
      781 gatcacgcgc aggtcggcgt cgcgcgccac ggcgaggctg cggccgtagg tctcgatcgc
      841 ctcgtccagg cggtcgagcc agaagtaggc cacgccggtg ttgcacagcg cgctgctcag
      901 gaggtaggga tcgaccgccg catgcccggc catcgcgacg attcggtgcg cgtgcgagag
      961 ggcgcgctcg tagtccttga cctcgaggta gagcaggctg aggttgttgt acgtggacag
     1021 gatctggcgc gtgtcgtcca ggcgctcgaa cgcgtgaagg gccttgtggc gaaacgagat
     1081 cgccagccgc ggctcgtcgg cgcggcgcca gtactcgccc caggcctggt cgagcatcgc
     1141 cgtcgtctcg agcgcggccg tcgcgcggcg cgactccgcg aggtccagca cggcgcgcga
     1201 gcgcgggtcg ttgcgcagca cgtagagcca ggccaggcgc acgagggcgg cgacatactc
     1261 cgaggcgacc tccgcggcgt ccggcgcctc gccttcggcc tggcgcagga agtcggcgct
     1321 ctcctcgaag caggcctgcg cgcggtcggt gtcgcgcgac tcgtggaacc agccgatcgc
     1381 gccgtagacg atgccgagca gcagcttgtc gcccgcctgc gaggcctcgc gcagcgcggc
     1441 gtcgtaggcg cgcgactcgc gctcgccggc attgcgggtg cgccagagcg cggcctcggc
     1501 caggtgcatg tcgaagcgct ggcgcggcgt cgacgcgagg ccggccaggc acgcgacgag
     1561 ggcgtcggtc tcgctggcgc tcgcgagctc gatgcggtag
//
LOCUS       DASVLN010000787         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_39505, whole genome shotgun sequence.
ACCESSION   DASVLN010000787 DASVLN010000000
VERSION     DASVLN010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39505"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VF453_18600"
     CDS             <1..582
                     /locus_tag="VF453_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689722.1"
                     /translation="ETPTVYLLSGWAKIGAPGGRAAVVDAPVVSVDAGGASIVVAPAA
                     DATIVFAEAGSLRVRRRASETVVPLQAGEMLSWNWDASRPKLSPRPSPGFVRDLPDAL
                     KVQLPSGYAAFKDKEAPPRALGAIAWADAQPWIDAEPVLREWAVHRWRALAKEPEFRR
                     ALVAGMKSHPEWEAALAASAPKRSRAASRGEGY"
     gene            617..1489
                     /locus_tag="VF453_18605"
     CDS             617..1489
                     /locus_tag="VF453_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEX7689723.1"
                     /translation="MKLLVKFNLVFLLVFLVGLAASSMIARNLLKQAAQDEVTDRARL
                     LMQHANAVSSYTAEEIKPLLATQMNYTFLPQSVPAFSAAEVLGKLQKDYPEYAFKSAM
                     LNPTNPRDRAVAWEADIITQFKQTPDLKEFIGERETPTGTSLYIARPIRITNPVCLSC
                     HSTPDAAPKTLIDKYGPANGFGWTLNDTLGVQVVSVPMTLPMRQADHALLVVMGVLTG
                     VFLLIGAALNFMLYRLVIRPVSRLSGIADRVSLGEFEAPEFEVRSKDEIGTLSASFNR
                     MRTSLAHAMKMLDA"
     gene            1503..>2389
                     /locus_tag="VF453_18610"
     CDS             1503..>2389
                     /locus_tag="VF453_18610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7689724.1"
                     /translation="MSTVPERIGKYEITQVLGQGSMGVVYKALDPHIQRPVAIKVIHK
                     ALLGDSGEARAIAARFLNEAKAVGRIAHPGVVAIYEFGEDAETAYIAMEFVNGRNLDD
                     ILAEVPVPTQEQTLHVMDGLLDALSHAHAQGVWHRDVKPANLMIAKGGQVKLTDFGIA
                     RVSDANLTQVSSMIGTPSYMSPEQFRGEGIDHRADLFASGVLLYRLLTGKRPFRGSSQ
                     EVMYKILQEDAPPPSQVTGGRSAPAFDAVVARAMAKNADDRYPSADAMRQALRDIAHG
                     KTGLPGAGGAQATNVVAVPR"
BASE COUNT          368 a          880 c          814 g          327 t
ORIGIN      
        1 gagaccccca cggtctacct gctcagcggc tgggccaaga tcggcgcgcc gggcgggcgc
       61 gccgcggtgg tcgacgcgcc ggtggtctcg gtggacgccg gcggcgcgag catcgtggtc
      121 gcccccgccg cggacgcgac catcgtcttc gccgaggccg gctccctgcg cgtgcgccgg
      181 cgcgcgagcg agacggtcgt tccgctgcag gccggggaga tgctgtcgtg gaactgggac
      241 gcgtcgcggc ccaagctctc gccgcgcccg agccccggct tcgtgcggga cctgccggac
      301 gcgctcaagg tgcagttgcc ctcgggctat gcggcgttca aggacaagga ggcgccgccg
      361 cgcgcgctcg gcgcgatcgc ctgggccgac gcccagccct ggatcgacgc cgagcccgtg
      421 ctgcgcgaat gggcggtgca ccgctggcgc gcgctcgcga aggagcccga gttccggcgc
      481 gcgctggtcg ccggcatgaa gtcccatccc gagtgggagg ccgcgctcgc cgcctccgcg
      541 cccaagcgct cccgcgccgc ctcgcgcggc gagggctact gaatcccgtc cccgcccatc
      601 cgccaaaggc gccgccatga agctcctcgt caagttcaac ctcgtgttcc tgctggtctt
      661 cctggtcggc ctcgccgcgt ccagcatgat cgcgcgcaac ctcctgaagc aggccgcgca
      721 ggacgaggtg accgaccgcg cgcgcctgct gatgcagcac gccaacgcgg tcagcagcta
      781 cacggccgag gaaatcaagc cgctgctcgc gacgcagatg aactacacct tcctgccgca
      841 gtcggtgccg gcgttctcgg ccgccgaggt gctgggcaag ctgcagaagg actaccccga
      901 gtacgcgttc aagtccgcga tgctcaaccc gaccaacccg cgcgaccgcg ccgtggcctg
      961 ggaggcggac atcatcacgc agttcaagca gacgcccgac ctgaaggagt tcatcggcga
     1021 gcgcgagacg cccacgggca cctcgctgta catcgcgcgc ccgatccgca tcaccaaccc
     1081 ggtctgcctg tcctgccaca gcacgcccga tgcggcgccc aagacgctga tcgacaagta
     1141 cggcccggcc aacggcttcg gctggacgct caacgacacg ctgggcgtgc aggtggtctc
     1201 ggtgccgatg acgctgccga tgcgccaggc cgaccacgcg ctgctggtcg tgatgggcgt
     1261 gctcaccggg gtgttcctgc tgatcggcgc ggcgctgaac ttcatgctct atcgcctcgt
     1321 catccggccg gtgagccggc tctcgggcat cgccgaccgc gtctcgctcg gcgagttcga
     1381 ggcgcccgag ttcgaggtgc gctccaagga cgagatcggc acgctgtcgg cctcgttcaa
     1441 ccgcatgcgc acgagcctcg cccacgcgat gaagatgctc gacgcctgat cccgtccgcg
     1501 ccatgtccac ggttcccgaa cgcatcggca agtacgagat cacccaggtg ctcggccagg
     1561 gctcgatggg cgtcgtctac aaggcgctcg acccgcacat ccagcggccg gtcgcgatca
     1621 aggtgatcca caaggcgctg ctgggcgaca gcggcgaggc cagggcgatc gccgcccgct
     1681 tcctcaacga ggccaaggcc gtgggccgca tcgcgcatcc gggcgtggtc gcgatctacg
     1741 agttcggcga ggacgccgag accgcgtaca tcgcgatgga gttcgtcaac ggccgcaacc
     1801 tcgacgacat cctcgcagag gtgccggtcc ccacccagga gcagacgctg cacgtgatgg
     1861 acggcctgct cgatgcgctg tcgcacgcgc acgcgcaggg cgtctggcac cgggacgtga
     1921 agccggccaa cctcatgatc gccaagggcg ggcaggtcaa gctcacggac ttcggcatcg
     1981 cgcgcgtctc cgacgccaac ctgacgcagg tgtcgtcgat gatcggcacg ccgagctaca
     2041 tgtcgcccga gcagttccgc ggcgagggca tcgaccatcg cgccgacctc ttcgcgagcg
     2101 gcgtgctgct gtaccgcctg ctcacgggca agcggccgtt ccgcggcagc tcgcaggagg
     2161 tcatgtacaa gatcctgcag gaagacgcgc cgccgccgag ccaggtcacc ggcggccgga
     2221 gcgcgcccgc gttcgacgcc gtcgtcgcgc gagcgatggc caagaacgcc gacgaccgct
     2281 atcccagcgc ggacgcgatg cgccaggcgc tgcgcgacat cgcccacggc aagacgggcc
     2341 tgcccggcgc gggcggcgcc caggcgacca acgtcgtcgc cgtgccgcg
//
LOCUS       DASVLN010000788         6011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_151395, whole genome shotgun sequence.
ACCESSION   DASVLN010000788 DASVLN010000000
VERSION     DASVLN010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151395"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /gene="mrdA"
                     /locus_tag="VF453_18615"
     CDS             complement(<1..1979)
                     /gene="mrdA"
                     /locus_tag="VF453_18615"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7689725.1"
                     /translation="MKSLTELRNIEVELDRFRARTLAAAAFVLLAFGLLVFRWFVLQV
                     VRHDDLAAQAEANRIGVVPIVPNRGLIVDRNGIVLATNYPAYTLEITPSKVTDLEATI
                     NEIAKVIEVTPRDRRRFARLAAEGKSFESVPIRTKLTDEEVARFVAQRFRFPGVEIKA
                     RLFRNYPHGELASHLIGYIGRINQEEKDKIAEDWSDEDQANYRGTEYIGKLGLEQSYE
                     SELHGVTGFEQIETSAGGRAVRQLASKPATPGNTLQLSIDIRLQALVEELYGNRRGAL
                     VAIDPRNGEVLAFVSKPTFDPNLFVDGIDADNWKELNESIDKPLLNRAIRGTYAPGST
                     YKPYMALAALTLGKRTPQWGMQDPGHFTFGGHVFRDDKPEGHGFVDMYRSIVVSCDVY
                     YFMLANDLGVDAIASFMQPFGFGQPTGIDLQGELRGVLPSTEWKRKAYRRPEQQKWYA
                     GETISLGIGQGYNSFTIMQLANALTILSTGGQRHTPHLVRDIVDVVNGTKHPVAPTPL
                     DPLPLNPEYVDVVRNAMVGVNREGTGAAAFLNAGYVSAGKTGTAQVADIKANEKYNAA
                     KVAEHLRDNALFISFAPADHPTIAIAMVVENVGFGAQYAGPIARRVMDYWIKGIYPTD
                     EDIAAVQAGTPGPPKTEGVPVALATNIRLGTNHAPG"
     gene            complement(2116..2631)
                     /gene="mreD"
                     /locus_tag="VF453_18620"
     CDS             complement(2116..2631)
                     /gene="mreD"
                     /locus_tag="VF453_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX7689726.1"
                     /translation="MPRGNDQLLLPVNPLFLWASLIGAMVLNMLPLGRVPALPDLLAL
                     TLVFWNVHQPRRVGIGAAFFFGLLMDVHQGAVLGQHAQAYTLLSFLAITIHRRLVWFS
                     IGEQALQILPLFFAAHAVSLVVRLLSSGSFPGWSLLLAPVFEAALWPVVSWILLAPQR
                     RAPDPDAQRPL"
     gene            complement(2634..3566)
                     /gene="mreC"
                     /locus_tag="VF453_18625"
     CDS             complement(2634..3566)
                     /gene="mreC"
                     /locus_tag="VF453_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX7689727.1"
                     /translation="MPLGTLDRRPPPLFHQGPSALTRLAVFSALALFLMAADTRFALT
                     RPLRAGLAILLVPVERAWQLPVEGVSAMGEYASGLHQAQADLDKAQRQLAAQSARAAA
                     VSELQAENARLRAIIDLRPRLTVKSTAAEVLYEASDPFSRKIFIDRGATSHVAPGSPV
                     IDEAGVLGQVTRVYPMSSEVTLLIDREAAIPVLNVRTHQRSAAFGADGSRMELRFMAG
                     NADVREGDVLQTSGVDGIYPPGLDVAKVVRVDRSADSSFARISLAPMASADGVRHVLV
                     LEPMAMQLPARPAEAEAEPASSPKGPRAIKGTKK"
     gene            complement(3730..4779)
                     /locus_tag="VF453_18630"
     CDS             complement(3730..4779)
                     /locus_tag="VF453_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7689728.1"
                     /translation="MFSSLRRYFSTDLAIDLGTANTLIYVRDKGIVLDEPSVVSIRHE
                     GGPHGKKIIQAVGKEAKAMLGKVPGNIEAIRPMKDGVIADFMVTEQMIKQFIKMVHPR
                     SLLSPSPRIIICVPCGSTQVERRAIKDAAEAAGASAVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGIVYKGSVRVGGDKFDEAIINYIRRNYGMLIGEQTAEN
                     IKMGIGSAFPGSEVREMEVKGRNLSEGVPRSFTISSNEVLEALTDPLNQIVSAVKNAL
                     EQTPPELGADIAERGMMLTGGGALLRDLDRLLAEETGLPVLVAEDPLTCVVRGCGMAL
                     ERMERLGAIFTSDER"
     gene            5214..5513
                     /gene="gatC"
                     /locus_tag="VF453_18635"
     CDS             5214..5513
                     /gene="gatC"
                     /locus_tag="VF453_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX7689729.1"
                     /translation="MSLTPDDVSRIAHLARLELSPPEGAAMLAQLNDFFRIVEQMSAV
                     DTSGLEPLHTPLSAIGEVALRLRPDAVTETDRRDANMANAPLAEDGLFLVPKVIE"
     gene            5510..>6011
                     /locus_tag="VF453_18640"
     CDS             5510..>6011
                     /locus_tag="VF453_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7689730.1"
                     /translation="MTTHELHEMGVAELSRALASKETSATEVALALLGRARADTHGAF
                     LAIDDEGAVAAAKSADALRAAGGEAASSPLLGVPIAHKDIFVTEGSATTAGSKMLEGY
                     RSPFDATVVARLKAAGAVSIGKLNCDEFAMGSSNENSAYGPVRNPWDAARVPGGSSGG
                     SAAAVAA"
BASE COUNT          865 a         2064 c         2173 g          909 t
ORIGIN      
        1 cctggggcgt ggttggtgcc caggcggatg ttggtcgcga gcgcgaccgg caccccttcc
       61 gtcttcggcg gcccgggcgt gccggcctgg accgcggcga tgtcctcgtc ggtcgggtag
      121 atgcccttga tccagtagtc catcacgcgg cgcgcgatcg gcccggcgta ctgggcgccg
      181 aagccgacgt tctcgaccac catcgcgatc gcgatcgtcg gatggtcggc cggcgcgaac
      241 gagatgaaca gcgcgttgtc gcgcaggtgc tcggcgacct tggccgcgtt gtacttctcg
      301 ttggccttga tgtcggcgac ctgcgccgtg ccggtcttgc ccgccgacac gtagccggcg
      361 ttcaggaagg ccgcggcgcc ggtgccctcg cggttgacgc cgaccatcgc gttgcgcacc
      421 acgtcgacgt actcgggatt gagcggcagc gggtcgagcg gcgtcggcgc gaccgggtgc
      481 ttcgtgccgt tgacgacgtc gacgatgtcg cgcaccaggt gcggcgtgtg gcgctggccg
      541 ccggtcgaca ggatcgtcag cgcattggcc agctgcatga tggtgaagct gttgtagccc
      601 tggccgatgc ccagcgagat cgtctcgccc gcataccact tctgctgctc gggccggcgg
      661 taggccttgc gcttccactc ggtcgatggc agcacgccgc gcagctcgcc ctgcaggtcg
      721 atgcccgtgg gctggccgaa gccgaagggc tgcatgaagc tggcgatcgc atcgacgccg
      781 aggtcgttgg cgagcatgaa gtagtagacg tcgcacgaga ccacgatcga gcggtacatg
      841 tcgacgaagc cgtggccctc gggcttgtcg tcgcggaaga cgtggccgcc gaacgtgaag
      901 tgcccgggat cctgcatgcc ccactgcggc gtgcgcttgc ccagcgtgag cgccgccagc
      961 gccatgtacg gcttgtacgt cgagcccggg gcgtaggtgc cgcggatggc gcgattgagc
     1021 agcggcttgt cgatcgactc gttgagctcc ttccagttgt ccgcgtcgat gccgtcgacg
     1081 aacaggttgg ggtcgaaggt cggcttggag acgaaggcca gcacctcgcc gttgcgcggg
     1141 tcgatcgcca ccagcgcgcc gcggcggttg ccgtacagct cctcgacgag cgcctgcagc
     1201 cggatgtcga tggacagctg cagcgtgttg ccgggcgtcg ccggcttgct cgcgagctgg
     1261 cgcaccgcgc ggccgccggc cgaggtctcg atctgctcga agccggtgac gccgtgcagc
     1321 tccgactcgt agctctgctc caggcccagc ttgccgatgt actcggtgcc gcggtagttg
     1381 gcctggtcct cgtccgacca gtcctcggcg atcttgtcct tctcctcctg gttgatgcgg
     1441 ccgatgtagc cgatcaggtg gctcgcgagc tcgccgtggg gatagttgcg gaacaggcgc
     1501 gccttgatct cgacgccggg gaagcggaag cgctgggcga cgaagcgggc gacttcctcg
     1561 tcggtgagct tggtgcggat cggcaccgac tcgaagctct tgccttcggc ggccaggcgc
     1621 gcgaagcggc ggcggtcgcg cggcgtcacc tcgatcacct tggcgatctc gttgatcgtg
     1681 gcctcgaggt cggtgacctt ggacggcgtg atctccagcg tgtaggcggg gtagttggtc
     1741 gccagcacga tgccgttgcg gtcgacgatc agcccgcggt tcggcacgat gggcacgacg
     1801 ccgatccggt tggcctcggc ctgggcggcg aggtcgtcgt ggcgcacgac ctgcagcacg
     1861 aaccagcgga acaccagcag gccgaaggcc agcagcacga aggccgccgc cgcgagcgtg
     1921 cgcgcgcgga agcggtcgag ctccacctcg atgttccgaa gctcggtcag agacttcatg
     1981 cccccacgct cgctggcgct cgctgccccc cgagggggcc ggccgcgacc cgaccggcac
     2041 agccggatcg agcgctccct tggtctttcc ttcggagcgc ctgagcggca gcgccggcaa
     2101 cgaaaagccg ctgagtcata gcggacgctg ggcgtcgggg tcgggcgcgc ggcgctgcgg
     2161 ggcgagcagg atccagctca cgaccggcca cagcgccgcc tcgaacaccg gcgcgagcag
     2221 cagcgaccag ccggggaagc tgccgctgga cagcaggcgc acgaccagcg acaccgcgtg
     2281 cgccgcgaag aacagcggca ggatctgcag ggcctgctcg ccgatagaga accagacgag
     2341 gcgccggtgg atcgtgatcg cgaggaagct caggagcgtg taggcctgcg cgtgctggcc
     2401 gagcaccgcg ccctggtgca cgtccatcag cagcccgaag aagaacgcgg cgccgatgcc
     2461 gacgcggcgc ggctggtgca cgttccagaa caccagcgtg agcgcgagca ggtcgggcag
     2521 cgccggcacg cgaccgagcg gcagcatgtt gagcaccatc gcgccgatca gcgaggccca
     2581 caggaacagc gggttgaccg gcagcagcag ctggtcgttg ccacgcggca tgatcacttc
     2641 ttcgtcccct tgatggcccg cgggcccttg ggcgaggacg ccggctcggc ctccgcctcg
     2701 gccggacgcg ccggcagctg catcgccatc ggctcgagca ccagcacatg gcgcacgccg
     2761 tcggccgagg ccatgggcgc gagcgagatg cgggcgaacg acgagtccgc gctgcggtcg
     2821 acccgcacca ccttcgcgac gtccagcccg ggcgggtaga tgccgtcgac gccgctggtc
     2881 tgcagcacgt cgccctcgcg cacgtcggcg ttgccggcca tgaagcgcag ctccatgcgc
     2941 gagccgtcgg cgccgaaggc cgcgctgcgc tggtgcgtgc gcacgttcag caccgggatc
     3001 gcggcctccc tgtcgatcag cagcgtcacc tccgaggaca tcggatagac gcgcgtgacc
     3061 tggcccagca cgcccgcctc gtcgatcacc ggcgagccgg gcgccacgtg gctggtggcg
     3121 ccgcggtcga tgaagatctt gcgcgagaac gggtcgctcg cctcgtacag cacctcggcg
     3181 gcggtcgact tcaccgtcag ccgcgggcgc aggtcgatga tggcgcgcag gcgggcattc
     3241 tcggcctgca gctcggagac cgcggccgcc ctggccgact gcgcggcgag ctggcgctgc
     3301 gccttgtcga ggtcggcctg ggcctggtgc aggccgctcg cgtactcgcc catcgccgag
     3361 acgccctcga ccggcagctg ccacgcccgc tcgaccggca ccagcaggat cgccagcccc
     3421 gcgcgcagcg gccgcgtgag cgcgaagcgc gtgtccgccg ccatcaggaa cagcgccagc
     3481 gcggagaaca ccgccagccg cgtcagcgcg gacggaccct ggtggaagag cggcgggggt
     3541 cggcgatcaa gggtgccgag cggcatgacg tgtcgcgccg cgtcatcgcc gcggcgccgc
     3601 tgggagggaa tcggctatcg atcgattatc gccaagccgc gttcccgtcg tcgtggggaa
     3661 tgtcgccacc cgtgaaggcg tggggcaagc ttcgaggccg cgcggcgaac cggcggccgg
     3721 cgtcgccggt cagcgctcgt cggaggtgaa gatcgcgccc aggcgctcca tgcgctcgag
     3781 cgccatgccg cagccgcgca ccacgcaggt cagcggatcc tcggccacga gcaccggcag
     3841 gccggtctcc tcggccagca ggcggtccag gtcgcgcagc agggcgccgc cgccggtgag
     3901 catcatgccg cgctcggcga tgtcggcacc cagctccggc ggcgtctgct cgagcgcgtt
     3961 cttcaccgcg gagacaatct ggttgagcgg gtcggtcagc gcctccagca cctcgttgga
     4021 gctgatggtg aagctgcgcg gcacgccctc ggacaggttg cgtcccttga cctccatctc
     4081 gcggacctcg gagcccggga aggccgagcc gatgcccatc ttgatgttct ccgccgtctg
     4141 ctcgccgatc agcatgccgt agttgcggcg gatgtagttg atgatcgcct cgtcgaactt
     4201 gtcgccgccg acgcgcacgc tgcccttgta gacgatgccg cccagcgaga tgacgccgac
     4261 ctccgtggtg ccgccgccga tgtcgacgac catcgaaccc gaggcttccg acaccggcag
     4321 gccggcgccg atcgcggcgg ccatcggctc ctcgatcagg tagacggcgc tcgcgcccgc
     4381 ggcctcggcg gcgtccttga tcgcgcggcg ttccacctgg gtcgagccgc agggcacgca
     4441 gatgatgatg cgcgggctcg gcgagagcag gctgcgcgga tgcaccatct tgatgaactg
     4501 cttgatcatc tgctcggtga ccatgaagtc ggcgatcacg ccgtccttca tcgggcggat
     4561 ggcctcgatg ttgcccggca ccttgccgag catcgccttg gcttccttgc cgacggcctg
     4621 gatgatcttc ttgccgtgcg ggccgccctc gtggcggatc gacacgaccg acggctcgtc
     4681 caggacgatg cccttgtccc gtacgtagat cagcgtgttg gcggtgccga ggtcgatcgc
     4741 gagatcggtg gagaagtagc ggcgcaggga ggagaacatt gggcggggat gtgggggtcg
     4801 cggggccttc gggcggtcga tctggcggga cgggctctca ggccatctgg atgggtgtgc
     4861 cttcggcgaa catgggcgtt gaggcgagga cgacgggcgg cgaagcggtc tggtctcgcg
     4921 ggccggggtt gccgaacctg ctgggcgggt ctcgatcggg gcgcgcgtgg aaggcgctgg
     4981 ggaaagcaca agcgctcgtc aaagcgcgcg aatctgggtc gtccggggcg ctttccgtcg
     5041 cgatctgacg tcgcatgagg aagctccgga ttaaccggag ataatacctc aagcgccctg
     5101 tccgatcgag gttgcacgac gtaactttct cgggccgcgc ctctcgccaa cgcggcggga
     5161 aggccgccgc ggaagacggc gcgccgcccc cgtcgccacc agccggatcc accatgtcac
     5221 tgacccccga cgacgtcagc cggatcgcgc atctcgcgcg gctcgagctg tcgccccccg
     5281 aaggcgccgc catgctggcg cagctgaacg acttcttccg gatcgtcgag cagatgagtg
     5341 ccgtcgacac ctccggcctc gagccgcttc acacgccgct gtcggccatc ggcgaggtcg
     5401 cgctgcgcct gcgtcccgac gccgtgaccg agacggaccg ccgcgatgcc aacatggcca
     5461 acgcgccgct ggccgaggac ggcctcttcc tcgtgccgaa ggtgatcgaa tgacgacgca
     5521 cgaattgcac gagatgggcg tggcggagct gtcgcgcgcc ctggcttcga aggagacctc
     5581 ggccaccgag gtcgcgctgg ccctgctcgg ccgcgcccgc gccgacacgc acggcgcgtt
     5641 cctcgcgatc gacgacgagg gcgccgtcgc ggccgcgaag tcggccgacg cgctgcgcgc
     5701 cgcgggcggc gaggccgcgt cgagcccgct gctgggcgtg ccgatcgcgc acaaggacat
     5761 cttcgtcacc gagggctcgg cgaccaccgc ggggtcgaag atgctcgagg gctaccgcag
     5821 cccgttcgac gccaccgtcg tcgcgcgcct gaaggccgcc ggcgcggtct cgatcggcaa
     5881 gctcaactgc gacgagttcg cgatgggctc gtccaacgag aactcggcct acggccccgt
     5941 gcgcaacccg tgggacgcgg cgcgcgtgcc gggcggctcg tcgggcggct ccgcggccgc
     6001 ggtcgcggcg c
//
LOCUS       DASVLN010000789         6121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_255939, whole genome shotgun sequence.
ACCESSION   DASVLN010000789 DASVLN010000000
VERSION     DASVLN010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255939"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2205
                     /locus_tag="VF453_18645"
     CDS             <1..2205
                     /locus_tag="VF453_18645"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7689731.1"
                     /translation="ASLLLAACAERDASALIDQPRAASAPAASASGALPQVSAMPGRG
                     YPFAAPHPVTNEYHGVAVRDDFQWLENWADPNAKAWVGAENAYSRRFLDAMPARAALH
                     ERLKALMSSTSSSYAGLVERGGVIFALKNAPPKQQPLLVTLKSVDDLASEHVVYDPNA
                     EAADGSLTIDFFKPSPDGRRVAVSVSKGGSEDGTLRILDVATGQALPDRIPRVTYPTG
                     GGDVAWSAGGAGLYYTQYPAPGTRPEADIHFFQQVFFHKLGTPATQDRHEVGDDFPRI
                     AETRLESSRDGRIVTAIVENGDGGDYALYLKTPDANGAGAWRRIAADADGVKDSRIGD
                     DGALYLLSRAGAPRGKLLRLPVSAKTQKVDWARVPVIVPQSEGSIEAYVPSGRRLYVA
                     ELAGGPSTLRTVDLRRHRAASVALPPVTGIADLADVGHGDVVANLTSYLAPPAWMHVG
                     TGRAKRSAMFVTSEATFNDAEVVREFAVSRDGTKVPVNIIRRKGTRLDGRNPTILYGY
                     GGFGVPMTPGFNPARRVWLDRGGVYVVANIRGGGEYGEAWHQAGNLTHKQNVFDDFIA
                     SAQYLIKAGYTQPARLGIMGGSNGGLLMGAALTQRPELFRAVVSTVGVYDMLRVELDP
                     NGAFNTTEFGTVKDKAQFEALYAYSPYRNVRDGTDYPAILMMTGDNDGRVNPAHSRKM
                     IARLQQADPAGKPILLLTSASSGHGIGTALSERIEQTVDQYGFLVDQLTGGQ"
     gene            complement(2243..3331)
                     /locus_tag="VF453_18650"
     CDS             complement(2243..3331)
                     /locus_tag="VF453_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX7689732.1"
                     /translation="MAGLAGCGGDGGDDGPPKVLIDSDYNTMSDDGQLGTMAAQLMAQ
                     GTIDVLGITVLSGNQWLMQGVADALKSVERMGVDARVGVYAGANTPLSHDYATVQAEL
                     AQYPSGDGYLGAWSTPEPHSQADLAAPPDGFATRAQVQATGAVDFIVDSIRKWPHEVT
                     ILAIGPLTNLALAFRQAPDIVPLVKRIIYMGGAIDVPGNTTATAEFNMWFDPQAAREV
                     LRQPIAQVLVPLDVTDTVILTQPIYDRIAHDPARQTVVTQIFRQLNGYGFSGTDGFEN
                     DPNYTQDIWDTLTIAYLVDPTLATQTTTRYVDVVTDFGPDDGRTVGTVDNPGGLQPIT
                     IVQRFDNARFFDLYVDLLTRPVPVRLPG"
     gene            3770..4240
                     /locus_tag="VF453_18655"
     CDS             3770..4240
                     /locus_tag="VF453_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689733.1"
                     /translation="MAIKWMGRAAFAASAMALGLAGCGGGSVDVAVTPPPAAPAFDVG
                     AQIDGVPIAHFDVLPGDAETVSVLAGDTFELDSDGAVVWDFSVGGSADIPAQAGGALV
                     YGDATVNETVVGDSRLLVSVDSSAPPGTAIPVTIYITSQDDPTQYATVDLVINS"
     gene            4460..5053
                     /locus_tag="VF453_18660"
     CDS             4460..5053
                     /locus_tag="VF453_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689734.1"
                     /translation="MLTLLGLKRLHVGPVDAEVGAGECVAVMGRSGAGKSVLLRMVAD
                     LDPHEGDAALEGVPAASLPAPEWRARVTYVGAESGWWSATVADHFAPGSDFSRFEDVG
                     IRPQAAAWPVSRLSTGERQRLALLRAITPRTRVLLLDEPTSGLDRDNIARVEVLLRGL
                     LARGLAIVLVTHDPAQAERLAHRRLELSEGRLVGAAA"
     gene            5050..5841
                     /gene="fetB"
                     /locus_tag="VF453_18665"
     CDS             5050..5841
                     /gene="fetB"
                     /locus_tag="VF453_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEX7689735.1"
                     /translation="MNPPPIGAWELAGAAGLVLANAGLSLVFEAGMGRTLVVAAIRAI
                     VQLVFVGYVLNAVFALRSPWLVGLVVLAMLAIAGFEIQSRQERRLRGAWRYGLGAGTT
                     LFATMVVGALSVAMLRPAPPYDPHVVIPLVGILLGSVMNGVSISLNAFNAGVVRERLA
                     IEAQLALGATRSQALGPLRRSALRGGMIPVVNQMSAAGIITLPGMMSGQILAGMDPLE
                     AAKYQALVLLLLAGGAALGAFATVWLAGRRVTDERHRLRLDRLVL"
BASE COUNT          800 a         2198 c         2236 g          887 t
ORIGIN      
        1 gcgagcctgc tgctcgcggc ctgcgccgaa cgcgacgcct ccgcgctgat cgatcagccc
       61 cgcgccgcct ccgcgccggc ggcctccgcc agcggcgcgc tgccgcaggt ctccgcgatg
      121 cccgggcgcg gctatccgtt cgcggcgccg catccggtga ccaacgagta ccacggcgtc
      181 gccgtgcgcg acgacttcca gtggctggag aactgggccg acccgaacgc caaggcctgg
      241 gtcggcgcgg agaacgcgta cagccgccgc ttcctcgacg cgatgcccgc gcgcgccgcg
      301 ctgcacgaga ggctcaaggc gctgatgtcg tccacctcca gctcgtacgc cgggctggtc
      361 gagcgcggcg gcgtgatctt cgcgctgaag aacgccccgc ccaagcagca gccgctgctc
      421 gtgacgctga agtcggtcga cgacctcgcg agcgagcacg tggtctacga cccgaacgcg
      481 gaggccgccg acggctcgct gacgatcgac ttcttcaagc cttcgccgga cggccggcgg
      541 gtcgcggtct cggtgtccaa gggcggcagc gaggacggca cgctgcgcat cctggacgtc
      601 gccacgggcc aggcgctgcc cgaccgcatc ccgcgcgtga cctacccgac cggtggcggc
      661 gacgtcgcct ggagcgccgg cggcgccggc ctgtactaca cccagtatcc ggcgccgggc
      721 acgcggcccg aggccgacat ccatttcttc cagcaggtgt tcttccacaa gctgggcacg
      781 cccgcgacgc aggatcgcca cgaggtcggc gacgacttcc cgcgcatcgc ggagacgcgg
      841 ctggagagct cgcgcgacgg ccgcatcgtc acggcgatcg tcgagaacgg cgacggcggc
      901 gactacgcgc tttacctgaa gacgcccgac gccaacggcg cgggggcgtg gcgtcgcatc
      961 gcggccgacg cggacggcgt caaggactcg cgcatcggcg acgacggcgc gctgtacctg
     1021 ctctcgcgcg cgggcgcgcc gcgcggcaag ctgctgcggc tgccggtgag cgcgaagacg
     1081 cagaaggtcg actgggcgcg cgtgccggtg atcgtgccgc agtccgaggg ctccatcgag
     1141 gcgtacgtgc cctcgggccg ccggctctac gtcgccgagc tcgcgggcgg cccgtcgacg
     1201 ctgcgcaccg tcgacctgcg caggcaccgc gcggcgagcg tcgcgctgcc gcccgtcacg
     1261 ggcatcgccg acctcgcgga cgtcggccac ggcgacgtcg tcgccaacct cacgagctac
     1321 ctcgcgccgc cggcctggat gcacgtcggc accgggcgcg cgaagcgcag cgcgatgttc
     1381 gtgacctcgg aagcgacctt caacgacgcc gaggtggtgc gcgagttcgc ggtctcccgg
     1441 gacggcacca aggtgcccgt caacatcatc cgccgcaagg gcacgcgcct ggacggccgc
     1501 aatcccacca tcctctacgg ctacggcggc ttcggcgtgc ccatgacgcc cggcttcaac
     1561 ccggcgcgtc gcgtgtggct ggatcgcggc ggcgtctacg tggtcgccaa catccgcggc
     1621 ggcggcgagt acggcgaggc ctggcaccag gccggcaacc tcacgcacaa gcagaacgtc
     1681 ttcgacgact tcatcgcctc ggcccagtac ctgatcaagg ccggctacac ccagcccgcg
     1741 cgcctgggga tcatgggcgg cagcaacggc ggcctgctga tgggcgcggc gctcacgcag
     1801 cggccggagc tgttccgcgc agtcgtcagc accgtgggcg tctacgacat gctgcgcgtc
     1861 gagctcgatc ccaacggcgc gttcaacacg accgagttcg gcaccgtgaa ggacaaggcg
     1921 cagttcgagg cgctgtatgc ctactcgccg taccgcaacg tgcgcgacgg caccgactac
     1981 ccggccatcc tcatgatgac cggcgacaac gacggccgcg tcaacccggc gcactcgcgc
     2041 aagatgatcg cgcggctgca gcaggccgac ccggccggca agccgatcct gctgctgacc
     2101 agcgcctcca gcggacacgg catcggcacg gcgctgtccg agcgcatcga gcagacggtc
     2161 gaccagtacg gcttcctggt cgaccagctg accggcggcc agtgacgccg ggcggaggcg
     2221 gccccagccg ccccgcgcgt ggtcagcccg gcaggcgcac gggcacgggc cgcgtcagca
     2281 ggtcgacgta gaggtcgaag aagcgcgcgt tgtcgaagcg ctgcacgatg gtgatcggct
     2341 gcaggccgcc cgggttgtcg acggtgccca ccgtgcgtcc gtcgtccggc ccgaagtcgg
     2401 tcacgacatc cacgtagcgc gtcgtcgtct gcgtggccag ggtcgggtcc accaggtagg
     2461 cgatggtgag cgtgtcccag atgtcctgcg tgtagttcgg gtcgttctcg aagccgtcgg
     2521 tgccgctgaa gccgtagccg ttgagctggc ggaagatctg cgtgacgacg gtctgcctgg
     2581 ccggatcgtg cgcgatccgg tcgtagatcg gctgcgtgag gatcacggtg tcggtgacgt
     2641 ccagcggcac cagcacctgc gcgatgggct ggcgcagcac ctcgcgggcg gcctgggggt
     2701 cgaaccacat gttgaattcg gccgtggccg tcgtgttgcc cggcacgtcg atggcgccgc
     2761 ccatgtagat gatgcgcttg acgagcggca cgatgtcggg cgcctggcgg aaggcgaggg
     2821 cgaggttggt gagcgggccg atcgccagga tggtgacctc gtgcggccac ttgcggatcg
     2881 agtcgacgat gaagtcgacc gcgcctgtcg cctgcacctg cgcgcgggtg gcgaagccgt
     2941 cgggcggcgc cgcgaggtcg gcctgcgagt gcggctcagg ggtgctccat gcgccgagat
     3001 acccatcccc gctggggtat tgcgcgagtt ccgcctggac ggtcgcgtag tcgtgcgaca
     3061 gcggcgtgtt cgcccccgcg tacacgccca cgcgcgcgtc gacgcccatg cgctcgaccg
     3121 acttgagcgc gtccgcgacg ccctgcatca gccactggtt gcccgagagc accgtgatcc
     3181 cgagaacgtc gatcgtgccc tgcgccatca gctgcgcggc catcgttccc agctggccgt
     3241 cgtcgctcat cgtgttgtag tcgctgtcga tgagcacctt cggcgggccg tcgtcgccgc
     3301 cgtcgccgcc gcagccggcg aggccggcca gggtcgcgcc cgcgccgatc gcgccaagcc
     3361 actggatgca agtccttcgc ttcaagagcc gtctccttgt cgtcgtcgtg cggcagcggg
     3421 caacgcgccg ccaggccgcc ccgcgaaggc ggctcgtgca cggggtggtc aggtggccgg
     3481 atggggtatc ggccgggtcg cgcaaacgtt tgcgcggcgc cggccattgg aagcgcggag
     3541 cccgcgacgg tcaactcggt gcgaaccccg caacggttgc gcaaccgccg agcaggtcgg
     3601 ggggcggtac ccggtcgggt atcgctccga cgcaagacct catgcctccc gggcacctag
     3661 gcttgcgact ggcgtgtcgg atccgtccga catccgccgg cccgtctcga acgttaaggc
     3721 tctcaaggcg ccgcgtcgcg gtgcgcgcgc aacttctaga ggagacctca tggcgatcaa
     3781 atggatgggc cgagccgcct tcgcggcgtc ggcgatggcc ctcgggcttg cgggctgcgg
     3841 cggcggcagc gtcgacgtcg cggtcacgcc accgccggcc gcgccggcct tcgacgtggg
     3901 ggcccagata gacggcgtcc cgatcgcgca cttcgacgtg ctgcccggcg acgccgagac
     3961 ggtatccgtc ctcgcgggcg acaccttcga gctcgactcg gacggcgcgg tggtctggga
     4021 cttctcggtc ggcggcagcg ccgacatccc ggcgcaggcg ggcggcgcac ttgtctacgg
     4081 cgacgccaca gtgaacgaga cggtggtggg cgattcgcgc ctcctggtgt ccgtggactc
     4141 gtcggcgccg ccgggcaccg ccatcccggt gaccatctac atcacctccc aggacgatcc
     4201 gacgcagtac gcgaccgtcg acctcgtcat caacagttga cggcggcatg cgtcgactcc
     4261 tgcagcgatg ttcgtcgtgc gctggcacgg cgcggcggcg tcccgccgga cagtcgaatg
     4321 cacgcgggcg gcgatgccgg cgcgcccatg ctgccaccgg ggagagcgcc aggagccggc
     4381 cgggccgccg aagccatcaa gttggagggc gaggcggcct tgcgccgaag ttcacgccac
     4441 gcgcgcggag cgaccggcca tgctgacgct gctcgggctg aagcggctgc acgtcggccc
     4501 ggtcgacgcc gaggtcggcg cgggggagtg cgtggcggtg atggggcgct ccggcgccgg
     4561 caagagcgtc ctgctgcgca tggtggccga cctcgatccg cacgaaggcg atgccgcgct
     4621 ggagggcgtg cccgcggcca gcttgccggc accggagtgg cgcgcccgcg tgacctacgt
     4681 cggggccgaa tcgggctggt ggtcggcgac ggtcgccgat cacttcgcgc cggggtcgga
     4741 cttctcgcgc ttcgaggacg tcggcatccg gccgcaggcg gcggcgtggc cggtctcgcg
     4801 cctgtccacc ggcgagcggc agcgcctcgc gctgctgcgc gcgatcacgc cgcgcacgcg
     4861 ggtgctgctg ctggacgagc cgacctccgg cctcgatcgc gacaacatcg cgcgcgtgga
     4921 agtgctgctg cgcgggctgc tcgcgcgcgg gctcgcgatc gtcctcgtca cgcacgaccc
     4981 cgcccaggct gagcggctgg cgcaccggcg cctcgagctg agcgaaggcc ggctggtcgg
     5041 ggccgccgca tgaatccgcc gccgatcggc gcgtgggagc tcgccggcgc ggcgggcctc
     5101 gtgctggcca acgccggcct gtcgctggtg ttcgaggcgg gcatggggcg cacgctcgtc
     5161 gtcgccgcca tccgcgcgat cgtgcagctc gtcttcgtcg gctacgtgct gaacgccgtg
     5221 ttcgcgctgc gctcgccgtg gctggtcggc ctggtggtgc tggcgatgct cgcgatcgcc
     5281 ggcttcgaga tccagtcgcg ccaggagcgc cgcctgcgcg gcgcctggcg ctacggcctg
     5341 ggcgccggca cgaccttgtt cgcgacgatg gtcgtcggcg cgctgagcgt tgccatgctc
     5401 cgcccggcgc cgccgtacga cccgcatgtc gtgatcccgc tggtcggcat cctgcttggc
     5461 agcgtcatga acggcgtgag catcagcctg aacgcgttca acgccggcgt cgtccgcgag
     5521 cgtctggcca tcgaggcgca gctcgcgctc ggcgccacgc gctcgcaggc gctcgggccg
     5581 ttgcgccgga gcgcgctgcg cggcggcatg atcccggtgg tcaaccagat gtcggccgcc
     5641 ggcatcatca cgctgccggg gatgatgagc ggccagatcc tcgccggcat ggatcccctc
     5701 gaggccgcga agtaccaggc gctggtgctg ctgctgctcg ccggcggcgc ggcgctcggg
     5761 gcgttcgcca ccgtctggct cgccggccgg cgcgtcaccg acgaacgcca ccggctgcgg
     5821 ctggatcgcc tggtgctttg aagaacccgt gaagcgatcc ggctggtcgg ccgggacgaa
     5881 gcccggctcc cgaggagccg gggaatggcc ggacacgggc gtctccccgc ggccggatcc
     5941 ctcgcgcagc ggctcgccgg tcagcgctcg ttctcgcggc cgttctcgtt ctcgcggccc
     6001 tcgtgcttct tgtcgccggt cttgcggcgt tcggcctcgg gcttgccctc gcggcgggcc
     6061 tgcggggcgt tggccgaggc cgggccctgc ggctggccgg cctgcccgtg ttcggccggg
     6121 c
//
LOCUS       DASVLN010000790        13053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_151464, whole genome shotgun sequence.
ACCESSION   DASVLN010000790 DASVLN010000000
VERSION     DASVLN010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151464"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VF453_18670"
     CDS             <1..369
                     /locus_tag="VF453_18670"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEX7689736.1"
                     /translation="IVSPHFDPAVVGRARALGLRSVPGVFTASEAFAAWRAGADALKI
                     FPAEAMTQAGLAGMTTVLPRDLPLWPVGSIVPGNMADWRRAGATGFGLGGGLFKPEFS
                     VQEIERRACDYLDAWSASSA"
     gene            complement(389..2917)
                     /locus_tag="VF453_18675"
     CDS             complement(389..2917)
                     /locus_tag="VF453_18675"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689737.1"
                     /translation="MAEALRAHDWGRTPLGPRDGWPTALKTLVALMLDSVQPMFLAWG
                     ARRIWLYNDAFIPILGNKHPQALGRPALDEVWAEAKADIEPLFDRVFRGEPVHMEDIA
                     LQLLRGQQAQEAHFAFSYSPVRGEDGEVQGLFGACIETTDRVLAERRRADDQQRQRDL
                     FAKAPGFIAVLRGPEHVFEFANEAYRRLAGGRALAGLPVREAFPDLEGQGFFELLDRV
                     YATNERYVASQVEVRLDRGAGHDPAPRYLDFIYEPVVDEAGRVTGIFVEGHDMTEAHR
                     AQAALRAAASRQERLVELDDRIRALDDPSEISYAAAELLGRSLGVSRAGYGNIDLEAE
                     TITIERDWNAPGIRSIAGVLHFRDYGTYIENLKRGETVVFDDASLDPRTQATADRLRA
                     IDAVSVINMPVTEHDGLVALLFLNHAHPRTWTEDELAFIRDVALRTRMAVERRRAERE
                     LRALATSLEQQVAARTAALQQIEETLRQSQKLEAMGQLTGGVAHDFNNLLAVISNNTA
                     LHARMSPVCEHSAQLAAIRRAADTGARLTRQLLAFARRQAVRPESISLADELPPLREM
                     LQTTLGSGIEVRLELAPGLPPVKADRSELELAIINLAMNARDAMPAGGSLAIRCEACE
                     GGVAIEARDTGTGIPPDVLQRVFEPFFTTKEPGRGTGLGLSQVYGFATQAGGRVAVDS
                     EVGRGTTVRMVLPASEQPEPAGACPASPDSRGHALDARILLVEDNADIRETTSELLRI
                     AGCRVSAAASGVEARDLLAGRGAPELDLVLTDIVMPGEMSGLDLAMWIRRRYPRLPLI
                     LATGYSAQVGAAAGQGFEILQKPIAPDALIEAVRRGLRQAATRD"
     gene            complement(3049..4077)
                     /locus_tag="VF453_18680"
     CDS             complement(3049..4077)
                     /locus_tag="VF453_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HEX7689738.1"
                     /translation="MSGELVLVTGGSGFVAGHCIRQLVERGFRVRTTVRSLAREAAVR
                     DQLALGERGAALAFFAADLTSDDGWDAAAAGCDFVLHVASPFPLHVPRHEDELVVPAR
                     EGALRVLRAARGAGVRRVVQTSSFAAVGYGHPETARPFDESDWTDVDGPGVTPYVKSK
                     TLAERAAWSFMGREGGDMELATVNPVGIFGPVMGSDYATSIEIVRRLLDGAMPGLPRL
                     TFGAVDVRDVADLHLRAMTHPEAAGERFLAVAGHFLELKEVAAILRRRLADAARRVPT
                     RELPDWLLKLVAVFDKSVGQVVPELGKRKDATSAKAQRVLGWTPRPAEDALVATAESL
                     RAHGLLKR"
     gene            complement(4217..6199)
                     /locus_tag="VF453_18685"
     CDS             complement(4217..6199)
                     /locus_tag="VF453_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX7689739.1"
                     /translation="MMPTPGNEIELLAPAKDADIGIEAVNHGADAVYIGGPAFGARAS
                     AGNEVGDIERLARHAHRYGAKIFVTLNTILRDDELEGARKMAWDVWRAGADALIVQDM
                     GLLELDLPPIQLHASTQTDIRTPEKAKFLQDIGFSQMVLARELDLRQIRAIAAATPRA
                     TLEFFIHGALCVAYSGQCYLSHAHTGRSANRGDCSQPCRLPYTVTDANGGIVAHDKHV
                     LSMKDNNQTANLDTLIDAGIRSFKIEGRYKDMGYVKNITAHYRRTLDALLERRPELQP
                     ASAGRCTFHFTPDPDRNFNRESTDYFVNGRQMDIGAFDTPKHAGLPVGHVVKVGADHF
                     EAELDAPDAQLHNGDGLTYLDLQKELVGVQADVVKKLDGPGNRWRIEPNRPVAELKDL
                     RSKPALHRNRDMAWDRLLTARSADRLVGVWMTLADVEGGLRLTVTDERGVAASADALL
                     QLEPARDAERARATLREHLGKLGGTIYALRELAIATREPWFVPASRLNALRRDALERL
                     DAVRLERREILPRATPVDPPATYPDDALSYLANVYNQPAHDFYAKHGVKVVEAAYESH
                     EELGEVSLMITKHCVRWSMSLCPKQAKGVTGVQGTVRAEPLVLHNGSEKLTLKFDCKP
                     CEMHVVGKIKKHVVNEALAVPMTFFRTRPAPTRPLR"
     gene            complement(6448..6954)
                     /locus_tag="VF453_18690"
     CDS             complement(6448..6954)
                     /locus_tag="VF453_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7689740.1"
                     /translation="MPDRPSTPSAPAARRSPAEQARFEAILADMFEQRIAFNQVLGLK
                     VAAIGAQSSSLRFDMRPELVGHFLYGRLHGGVISATLDATAGLALMAAMAERHAADTA
                     DQVVHRFSRLGTIDLRVDFLRQGVGKHFVATGEVTRLGGRVGSVLMKLVNDDGVLIAT
                     GAAAYMVA"
     gene            7007..7816
                     /locus_tag="VF453_18695"
     CDS             7007..7816
                     /locus_tag="VF453_18695"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HEX7689741.1"
                     /translation="MNAIARPIPAPAAARPAAGVRFDRLDAWRGVAIVWMACFHFSFD
                     LNLHGLLRPAQAFLGDPFWMTQRACIVSMFLLGAGAGQAVAVDAGQGWRRFWRRWAQV
                     AGCALLVSLATIPMFPMSWISFGVLHGVAVMLLLLRMLGTRLPPARLARSPLACGLLA
                     AVAVGAWLLPRFVQSPLFDTRLTNWVGLVTRLPITEDYVPVFPWLAMVLAGYLAQSLL
                     LVHARAFAAGPVPALVRPLAVLGRWSLSFYMLHQPILLGSLLLFLNLSRHP"
     gene            7813..8559
                     /gene="rlmB"
                     /locus_tag="VF453_18700"
     CDS             7813..8559
                     /gene="rlmB"
                     /locus_tag="VF453_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX7689742.1"
                     /translation="MSNPRVLYGFHAITVRMKTAPESILELHLEAGRRDARMRSFAER
                     ARELGIRIVESDHERLAKMSGGPRHQGVVARVEEVKMPHSLDEVVENIEGPPLLLVLD
                     GVTDPHNLGACLRVADGAGAHAIVAPKDHAVGVNATVAKVASGAAETVPYLMVTNLAR
                     SLTELKEHDIRIVGTSEDAEATLYDLDLTQPTAFVLGAEGDGMRQLTRKTCDQLVRIP
                     MAGAVESLNVSVAAAVCLYEALRQRRKAAA"
     gene            complement(8560..9060)
                     /locus_tag="VF453_18705"
     CDS             complement(8560..9060)
                     /locus_tag="VF453_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX7689743.1"
                     /translation="MFGQFVLLSLLSIGGAISTAPEMHRYLVEQHHWMTDAQFSASVA
                     IAQAAPGPNLLFVAVLGWGVAGFLGMCVTMLGILLPSTTLALFAARWGEARRETRGVR
                     AFVSGMAPVTVGLVLSTGAILGKPAYASPWLDVLIVATVVVMLRTKLSPLWLIAVGGV
                     VGALVG"
     gene            complement(9117..9764)
                     /locus_tag="VF453_18710"
     CDS             complement(9117..9764)
                     /locus_tag="VF453_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX7689744.1"
                     /translation="MTSPDLPPAAPPSPPAASPAAGGPASILDEPSPRAPQSTGELFR
                     VFTRLALQGFGGVLPVAQRELVERERWLTREQFVEMLAVAQVLPGPNVVNLSLMFGDR
                     AFGWRGGLAALAGMLAAPLGVVLVLTLVYLHFASDPVVAGALRGMGAVAAGLILSTGL
                     KLLTALRRNVLGAGLAWAFAGATVLAIAVARVPLIWALAVLGGAAFALAWRRLPR"
     gene            complement(9891..10898)
                     /locus_tag="VF453_18715"
     CDS             complement(9891..10898)
                     /locus_tag="VF453_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX7689745.1"
                     /translation="MNIIILDDYQDAVRKLPCAAKLSELNAKVFTNTVKGIGQLSVRL
                     REAEVLVLIRERTRFPRQLLEKLPKLRLIAQTGRVGEHIDVAACTQLGIAVAEGVGAP
                     YAPAELTWALIMASMRRLPQYIANLKHGAWQQSGLKAASMPPNFGVGMILRGKTLGIW
                     GYGRIGKLVAGYGHAFGMDVAVWGSDDARARAIADGYRALGSREELFQTADVLSVHLK
                     LGDATRHAVTLNDLLQMKPTALFVNTSRAELIEENALVAALNRGRPGMAAVDVFESEP
                     ILAGHPLLRLENAVCTPHIGYVEQASYELYFGAAFDNVVNFINGNPTNVVNPEALKVL
                     R"
     gene            complement(11208..11630)
                     /locus_tag="VF453_18720"
     CDS             complement(11208..11630)
                     /locus_tag="VF453_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689746.1"
                     /translation="MSIEPRRYDPLGLAELDPALVAEADALAAQFDAPGHAGLPADRT
                     ARIAARHAFVGMKRLFLRAVENLEHRKGAWLRAQVRAAEDPVDLWLLRGPLLAALRED
                     DREDTRKLRAELYRTLDTTFPAALRTARMSAQPAAAWR"
     gene            complement(11620..12048)
                     /locus_tag="VF453_18725"
     CDS             complement(11620..12048)
                     /locus_tag="VF453_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689747.1"
                     /translation="MSTALFARILRKPVAPTTLRMNVCPPDLCAPRGSLWNATLRWLV
                     GNDLEAVPALRTPLERARHEFVEAMVGLLDADTEDLLRRAQHARSLRELWHLRSELYT
                     MIARRVSQVEAEARLARVNQHFPTRAQRIVQPAPEAADVH"
     gene            complement(12199..>13053)
                     /locus_tag="VF453_18730"
     CDS             complement(12199..>13053)
                     /locus_tag="VF453_18730"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX7689748.1"
                     /translation="GLAHAAGPTAVDDAGHEIRLDRPARRIVTLAPHLTELAYAAGAG
                     DAVVAVGRYSDYPREALAKPVVGDAFAVDYEALAKLRPDLVLAWGSGTPQRFKDKLRE
                     LGWPVMEIEIRDVGGLESALRRLGTLAGTAQVADARARAIAADWTTLQREYAGRRPVR
                     VFFQLWDAPLMTLSGAHLISSAIAACGGRNVFADLPTLTASVSWEAAVQRDPELVVTG
                     GSPSEPAKPGRWADFPQVSATRHGEFARIDGDLIARSGPRFVDGARELCDAIDRARNG
                     QPQPGPAR"
BASE COUNT         1667 a         4647 c         4891 g         1848 t
ORIGIN      
        1 atcgtctcgc cgcacttcga ccccgccgtg gtcggccgcg cgcgcgcgct gggcctgcgc
       61 agcgtgccgg gcgtcttcac ggcttccgag gccttcgccg cctggcgcgc gggggccgac
      121 gcgctgaaga tcttccccgc ggaggcgatg acgcaggcag gcctcgcggg catgaccacc
      181 gtgctgccca gggacctgcc gctgtggccg gtcggcagca tcgtgccggg caacatggcc
      241 gattggcggc gcgccggcgc gaccggcttc ggcctcggcg gcggcctgtt caagcccgag
      301 ttctccgtgc aggagatcga gcgccgcgcc tgcgactacc tcgacgcctg gagcgcgtcg
      361 agcgcctgag ggtcgcgccg cggcgccttc aatcgcgcgt cgcggcctgc cgcaggccgc
      421 ggcgaaccgc ctcgatcaac gcgtcgggcg cgatgggctt ctgcaggatc tcgaagccct
      481 ggccggcggc ggcgcccacc tgcgcgctgt agcctgtcgc gaggatcagc ggcagcctcg
      541 gatatcggcg ccggatccac atcgcgagat ccagcccgct catctcgccc ggcatcacga
      601 tgtcggtcag cacgaggtcc agctccggcg cgccgcgccc ggccagcagg tcgcgggcct
      661 cgaccccgct ggcggcagcg ctgacgcggc agcccgcgat gcgaagcagc tcgctcgtcg
      721 tctcgcggat gtcggcgttg tcctcgacga gcaggatccg tgcgtccagc gcgtggcccc
      781 gcgagtccgg gctggcaggg caggcgccgg ccggctcggg ctgctcgctc gcgggcagca
      841 ccatgcgcac cgtcgtgccg cggcccacct cgctgtcgac ggccacgcga ccgccggcct
      901 gggtggcgaa cccgtagacc tggctgagcc cgaggcccgt cccccggccc ggttccttgg
      961 tcgtgaagaa cggctcgaag acgcgctgca ggacgtcggg cgggataccc gttcccgtat
     1021 cgcgcgcctc gatggcgacg ccgccttcgc aggcctcgca gcggatcgcc agcgaaccgc
     1081 cggcgggcat cgcgtcgcgc gcgttcatcg ccaggttgat gatggcgagc tccagctcgg
     1141 agcggtcggc cttcacgggc ggcaggcccg gcgcgagttc cagccgtacc tcgatgccgc
     1201 tgcccagcgt cgtctgcagc atctcgcgca ggggcggcag ctcgtcggcg aggctgatgg
     1261 actccgggcg cacggcctgg cggcgcgcga aggccagcag ctgccgggtc aggcgcgcgc
     1321 cggtgtcggc ggcgcggcgg atggcggcca gctgcgcgct gtgctcgcag acgggcgaca
     1381 tccgcgcatg cagcgccgtg ttgttggaga tgacggcgag caggttgttg aagtcgtgcg
     1441 cgacgccgcc ggtgagctgc cccatcgcct ccagcttctg gctctggcgc agcgtctcct
     1501 cgatctgctg cagcgccgcg gtgcgcgccg cgacctgctg ctccagcgac gtcgcgagcg
     1561 cgcgcagctc gcgttccgcg cgccggcgct ccaccgccat gcgcgtgcgc agcgcgacgt
     1621 cgcggatgaa cgccagctcg tcctccgtcc aggtgcgcgg gtgcgcgtgg ttcaggaaca
     1681 gcagggcgac caggccgtca tgctcggtga cgggcatgtt gatcaccgac accgcgtcga
     1741 tggcgcgcag ccggtccgcg gtggcctggg tgcgcggatc gaggctcgcg tcgtcgaaca
     1801 cgacggtctc gccgcgcttg aggttctcga tgtaggtgcc gtagtcgcgg aagtgcagca
     1861 cgcccgcgat gctgcggatg cccggcgcgt tccagtcccg ctcgatggtg atggtctccg
     1921 cctcgaggtc gatgttgccg tagccggcac ggctcacgcc gaggctgcgg ccgagcagct
     1981 cggccgcggc gtaggagatc tcggacgggt cgtcgagcgc ccggatgcgg tcgtcgagct
     2041 cgacgagccg ctcctggcgc gacgccgccg cgcgcagggc ggcctgggcg cggtgggcct
     2101 cggtcatgtc gtggccctcg acgaagatgc cggtcacgcg ccccgcctcg tcgacgaccg
     2161 gctcgtagat gaagtcgagg tagcggggcg ccggatcgtg ccccgcgccg cggtccaggc
     2221 gcacctcgac ctgcgaggcg acgtagcgct cgttggtcgc gtagacgcgg tcgagcagct
     2281 cgaagaagcc ctgtccctcg aggtccggga aggcctcgcg caccggcagc cccgccagcg
     2341 cccggccgcc ggccagtcgc cggtacgctt cgttggcgaa ctcgaagacg tgctcgggtc
     2401 cccgcagcac cgcgatgaag ccgggcgcct tggcgaacag gtcgcgctgg cgctgctggt
     2461 cgtcggcgcg gcgccgctcg gccagcacgc ggtcggtggt ctcgatgcag gcgccgaaca
     2521 gcccctgcac ctcgccgtcc tcgccccgca ccgggctgta cgagaacgcg aagtgcgcct
     2581 cctgcgcctg ctggccgcga agcagctgca gcgcgatgtc ctccatgtgg accggctcgc
     2641 cgcggaacac gcggtcgaac agcggctcga tgtcggcctt cgcctcggcc cacacctcgt
     2701 ccagcgccgg ccggccgagc gcctgcgggt gcttgttgcc gaggatgggg atgaaggcgt
     2761 cgttgtagag ccagatgcgc cgcgcgcccc aggcgaggaa catcggctgc acggagtcca
     2821 gcatcagcgc gaccagcgtc ttcagtgccg tgggccagcc gtcgcgcggc cccagcggcg
     2881 tccggcccca gtcgtgcgcg cggagggcct ccgccatggt gccgcccagc tgcgggggcg
     2941 acggcgatgg ggaggtggcg ggcgtcggcg gcatcggggc ggcgtgggtc gagccgccat
     3001 catgcccgac gcgcgcgttc gccgccaccg gcgtcgcgcc ggcggtgctc agcgcttgag
     3061 caggccgtgc gcccgcaggc tctcggccgt cgcgacgagc gcgtcctccg caggccgcgg
     3121 cgtccatccc agcacgcgct gcgccttcgc ggaggtggcg tccttgcgct tgccgagctc
     3181 cggcacgacc tggccgaccg acttgtcgaa gacggccacc agcttcagca gccagtccgg
     3241 cagttcgcgc gtgggcacgc ggcgggcggc atcggcgagc cggcgcctga ggatggccgc
     3301 gacctccttg agctcgagga agtgccccgc gacggccagg aagcgctcgc ccgcggcctc
     3361 gggatgcgtc atcgcgcgca ggtgcaggtc ggcgacgtcg cgcacgtcga ccgcgccgaa
     3421 ggtcaggcgc ggcagcccgg gcatcgcgcc gtcgagcagg cgccggacga tctcgatcga
     3481 ggtcgcgtag tccgacccca tcacgggccc gaagatgccc accggattga ccgtcgcgag
     3541 ctccatgtcg ccgccctcgc ggcccatgaa gctccaggcg gcgcgctcgg cgagcgtctt
     3601 cgacttcacg tagggcgtca cgcccgggcc gtcgacgtcg gtccagtccg attcgtcgaa
     3661 gggccgcgcg gtctcgggat ggccgtagcc caccgcggcg aaggacgagg tctgcaccac
     3721 ccgcctcacg cccgcgccgc gggccgcgcg cagcacgcgc agcgcgccct cgcgcgcggg
     3781 aacgaccagc tcgtcctcgt gcctgggcac gtgcagcgga aagggcgagg cgacgtgcag
     3841 cacgaagtcg cagccggcgg cggcggcgtc ccagccgtcg tcggacgtca ggtcggcggc
     3901 gaagaaggcg agtgccgcgc cgcgttcgcc gagcgccagc tggtcgcgca ccgcggcctc
     3961 gcgcgcgagc gagcgcaccg tcgtgcgcac gcgaaagccc cgttcgacca gttgccggat
     4021 gcagtgcccc gccacgaagc cggagccgcc cgtgaccaat accagttcgc cgctcatgtc
     4081 cgaagttgtc cccaggtggt gagtggcgcg aatcctaagc ttgaagccgg gtccaaggtc
     4141 aagcggcacc acggcccgcc tggacgcgca ttccacgctg ccggtgcgcg cgccgcgggg
     4201 acgtgcgagg acgtgttcag cgcagcggcc gcgtcggcgc cgggcgcgtg cggaagaagg
     4261 tcatcggcac cgccagggcc tcgttgacca cgtgcttctt gatcttgccg acgacgtgca
     4321 tctcgcacgg cttgcagtcg aacttcagcg tcagcttctc gctgccgttg tgcagcacca
     4381 gcggctcggc gcgcaccgtg ccctgcacgc cggtgacgcc cttggcctgc ttcgggcaca
     4441 gcgacatcga ccagcgcacg cagtgcttgg tgatcatcag cgagacctcg ccgagctcct
     4501 cgtggctctc gtaggcggcc tcgacgacct tgacgccgtg cttcgcgtag aagtcgtgcg
     4561 cgggctggtt gtagacgttg gcgaggtagc tgagggcgtc gtcggggtag gtggcgggcg
     4621 ggtcgacggg cgtggcgcga ggcaggatct cgcgccgttc caggcgcacg gcgtccaggc
     4681 gctcgagcgc gtcgcggcgc agcgcgttca ggcggctcgc gggcacgaac cagggctcgc
     4741 gcgtcgcgat ggccagctcg cgcagcgcgt agatcgtgcc gcccagcttg cccaggtgct
     4801 cgcgcagcgt cgcgcgggcg cgctcggcgt cgcgcgcggg ctccagctgc agcaacgcgt
     4861 cggccgacgc ggccacgccg cgctcgtcgg tcacggtcag gcgcaggccg ccctcgacgt
     4921 cggcgagcgt catccacacg cccacgaggc ggtcggccga cctggccgtc agcagccggt
     4981 cccacgccat gtcgcggttg cggtgcagcg ccggcttcga gcgcaggtcc ttgagttcgg
     5041 cgaccggccg gttcggctcg atgcgccagc ggttgccggg gccgtcgagc ttcttcacga
     5101 cgtccgcctg cacgccgacg agctccttct ggaggtcgag gtaggtcagg ccgtcgccgt
     5161 tgtgcagctg cgcatccggt gcgtcgagct cggcctcgaa gtgatcggcc ccgaccttca
     5221 cgacatggcc gaccggcagg cccgcgtgct tgggcgtgtc gaaggcgccg atgtccatct
     5281 ggcggccgtt gacgaagtag tcggtcgact cgcggttgaa gttgcggtcg gggtcgggcg
     5341 tgaagtggaa ggtgcagcgg cccgccgagg ccggctgcag ctcgggccgc cgctccagca
     5401 gcgcgtcgag cgtgcggcgg tagtgggcgg tgatgttctt cacgtagccc atgtccttgt
     5461 agcggccctc gatcttgaag ctgcggatcc ccgcgtcgat cagcgtgtcg aggttggccg
     5521 tctggttgtt gtccttcatc gacagcacgt gcttgtcgtg cgcgacgatg ccgccgttgg
     5581 cgtcggtgac cgtgtagggc aggcggcagg gctgcgaaca gtcgccacgg ttggcgctgc
     5641 ggccggtgtg cgcgtggctc aggtagcact ggccgctgta ggccacgcac agcgcgccgt
     5701 ggatgaagaa ctccagcgtc gcgcgcggcg tcgcggccgc gatggcgcgg atctggcgca
     5761 ggtcgagctc gcgcgccagc accatctgcg agaagccgat gtcctgcagg aacttcgcct
     5821 tctcgggggt gcggatgtcg gtctgcgtgc tcgcgtggag ctggatcggc ggcaggtcga
     5881 gctcgagcag gcccatgtcc tgcacgatga gcgcgtcggc cccggcgcgc cagacgtccc
     5941 aggccatctt gcgcgcgccc tcgagctcgt cgtcgcgcag gatggtgttg agcgtgacga
     6001 agatcttggc gccgtagcgg tgcgcatggc gggcgaggcg ctcgatgtcg ccgacctcgt
     6061 tgcccgccga cgcccgcgcg ccgaacgccg gcccgccgat gtagacggcg tccgcgccgt
     6121 ggttgacggc ctcgatgccg atgtcggcgt ccttcgcggg cgcgagcagc tcgatctcgt
     6181 tgccgggcgt cggcatcatg gcggaggccg cggaggcggc aggggcgggc gaagtcatgg
     6241 cggcggattc cggtgggcgc ccggcggtcg cgggcggcgg gctcggctcg ggcggccgcg
     6301 gcggtggccg cgatgcgagg cgatgcccgg cggtgcgccg aggacgtgga aggccggcgc
     6361 ggcgaaaggc ggattatcgc agggatgcgg gttttccgca gccctggcgg gcgggcgccc
     6421 tgcttgcgaa gcccccggcg gcggccctca cgcgaccatg tacgcggccg cgccggtggc
     6481 gatcagcacg ccgtcgtcgt tgacgagctt catgagcacc gagccgaccc gcccgcccag
     6541 ccgcgtgacc tcgccggtgg cgacgaagtg cttgccgaca ccctggcgca ggaagtcgac
     6601 gcgcaggtcg atcgtcccca ggcgcgagaa gcggtgcacg acctggtcgg ccgtgtcggc
     6661 ggcgtggcgc tcggccatcg cggccatgag cgcgaggccg gcggtcgcgt cgagcgtcgc
     6721 gctgatcacg ccgccgtgca gccggccgta gaggaagtgg ccgacgagct cgggacgcat
     6781 gtcgaagcgc agcgacgagg actgcgcgcc gatggccgcg accttcagcc ccagcacctg
     6841 gttgaaggcg atgcgctgct cgaacatgtc ggcgaggatg gcctcgaagc gggcctgctc
     6901 ggcggggctg cggcgggcgg cgggcgcgga cggggtgctg ggtcggtcgg gcatggcgcg
     6961 catgttagcg agccggcggc gcgcgccgtt gaggtatggt ccggcggtga acgcgatcgc
     7021 ccgacccatc cccgcgcccg cggccgcgcg tccggccgcg ggcgtgcgct tcgaccgcct
     7081 cgacgcctgg cgcggcgtgg ccatcgtctg gatggcctgc ttccacttct cgttcgacct
     7141 caacctgcac ggcctgctgc ggccggcgca ggccttcctc ggcgacccgt tctggatgac
     7201 gcagcgtgcg tgcatcgtct cgatgttcct gctcggcgcg ggggcggggc aggcggtggc
     7261 ggtggacgcc gggcagggct ggcgccggtt ctggcgccgc tgggcgcagg tggcgggctg
     7321 cgccctcctg gtctcgctcg cgacgatccc catgttcccc atgagctgga tcagcttcgg
     7381 cgtgctgcac ggcgtcgccg tcatgctgct gctgctgcgc atgctcggca cgcggctgcc
     7441 gccggcgcgg ctcgcccgct cgccgctcgc atgcggcctg ctggcggccg tcgcggtcgg
     7501 cgcgtggctg ctgccgcggt tcgtgcagtc gccgctgttc gacacccggc tgacgaactg
     7561 ggtcggcctc gtgacgcgac tgccgatcac ggaggactac gtgcccgtct tcccctggct
     7621 cgcgatggtg ctcgcgggct acctcgcgca gagcctgctg ctcgtgcatg cgcgcgcgtt
     7681 cgcggcgggg ccggtgcctg cgctggtgcg gccgctggcc gtgctggggc gctggtcgtt
     7741 gagcttctac atgctgcacc agccgatcct gctaggctcg ctgctccttt tcctgaacct
     7801 gtcccgccat ccatgagcaa tccccgcgtc ctctacggct tccatgcgat caccgtgcgc
     7861 atgaagaccg cgcccgagtc catcctcgag ctgcacctcg aggccggccg ccgcgacgcg
     7921 cgcatgcgct cgttcgcgga gcgcgcgcgc gagctgggca tccgcatcgt cgagtcggac
     7981 catgagcggc tcgcgaagat gtcgggcggg ccgcgccacc agggcgtggt cgcgcgcgtc
     8041 gaggaagtga agatgccgca ctcgctcgac gaggtggtcg agaacatcga gggcccgccg
     8101 ctgctgctgg tgctggacgg cgtgaccgac ccgcacaacc tcggcgcctg cctgcgcgtg
     8161 gccgacggcg ccggcgcgca cgcgatcgtc gcgcccaagg accacgccgt cggcgtcaac
     8221 gcgacggtcg ccaaggtggc cagcggtgcg gccgagacgg tgccctacct catggtcacc
     8281 aacctcgcgc gctcgctgac cgagctgaag gagcacgaca tccgcatcgt cggcacgagc
     8341 gaggacgccg aggccacgct ctacgacctc gacctgacgc agccgacggc gttcgtgctc
     8401 ggcgccgagg gcgacggcat gcgccagctc acgcgcaaga cctgcgacca gctggtgcgc
     8461 atcccgatgg cgggagcggt cgagagcctg aacgtgtcgg tggcggccgc cgtctgcctg
     8521 tacgaggcgc tgcgccagcg caggaaggcc gcggcctgat cagccgacca gcgctccgac
     8581 cacgccgccc accgcgatga gccacagcgg gctcagcttc gtgcgcagca tcaccacgac
     8641 ggtcgcgacg atcagcacgt ccagccacgg gctcgcgtag gccggcttgc cgaggatcgc
     8701 gccggtcgac agcacgaggc cgacggtcac cggcgccatg ccgctcacga aggcgcgcac
     8761 gccgcgcgtc tcgcgccgcg cctcgcccca gcgtgcggcg aacagggcga gcgtcgtcga
     8821 gggcagcagg atgcccagca tcgtgacgca catgcccagg aagccggcga cgccccagcc
     8881 cagcaccgcg acgaacagca ggttcggccc gggcgcggcc tgcgcgatcg cgaccgaggc
     8941 gctgaactgc gcgtcggtca tccagtggtg ctgctcgacg aggtagcggt gcatctcggg
     9001 cgcggtgctg atcgcgccgc cgatggacag cagcgacagc agcacgaact ggccgaacat
     9061 cccggcccag tcgcccgcgg cgagcaccgg cggcgccgtc acgacgaccg gcgccgtcac
     9121 cgcgggagcc tccgccatgc cagcgcgaac gccgcgccgc cgaggaccgc cagcgcccag
     9181 atgagcggca cccgcgcgac cgcgatcgcc agcaccgtcg cgcccgcgaa cgcccaggcg
     9241 aggccggcgc cgagcacgtt gcgcctgagc gcggtgagca gcttcaggcc ggtcgacagg
     9301 atcagccccg cggcgacggc gcccatcccg cgcagcgcgc cggcgaccac cgggtcgctc
     9361 gcgaagtgga ggtagacgag cgtcagcacg agcaccacgc ccaggggcgc cgccagcatg
     9421 ccggccagcg ccgcgagccc gccgcgccag ccgaacgcac ggtcgccgaa catcagcgac
     9481 aggttgacga cgttgggccc gggcagcacc tgggcgacgg ccagcatctc gacgaactgc
     9541 tcgcgcgtga gccagcgttc gcgctcgacg agttcgcgct gcgccaccgg cagcacgccg
     9601 ccgaagccct gcagcgccag gcgcgtgaag acgcggaaca gctcgccggt cgactgcggc
     9661 gcccgcgggg acggttcgtc caggatcgac gccgggccgc cggcggccgg ggacgccgcc
     9721 ggtggcgagg gcggggcggc gggcggcagg tcgggcgagg tcatcggctg caaggcgcgg
     9781 ggaacgtggc gacagcgtac tccggcgcgc gccggcaggt atgtcttatg tcttatacaa
     9841 gacttggcga ctcggccttt cgcgaggctt gcgcggcccc gcgccgggcg tcagcgcagg
     9901 accttcagcg cctccggatt gacgacgttg gtcgggttgc cgttgatgaa gttgacgacg
     9961 ttgtcgaacg cggcgccgaa gtacagctcg tagctcgcct gctcgacgta gccgatgtgc
    10021 ggcgtgcata ccgcgttctc cagccgcagc agcgggtggc cggcgaggat gggctcgctc
    10081 tcgaagacgt cgaccgcggc catgcccggc cggccgcggt tcagcgcggc gaccagcgcg
    10141 ttctcctcga tcagctcggc gcgcgaggtg ttcacgaaca gggcggtcgg cttcatctgc
    10201 agcaggtcgt tgagcgtgac ggcgtggcgc gtggcgtcgc ccagcttcag gtgcacgctc
    10261 agcacgtcgg cggtctggaa cagttcctcg cgcgagccga gcgcgcggta gccgtcggcg
    10321 atcgcgcggg cgcgggcgtc gtcggagccc cacaccgcga cgtccatccc gaacgcatgc
    10381 ccgtagcccg ccaccagctt gccgatgcgg ccgtagcccc agatgcccag cgtcttgccg
    10441 cgcaggatca tgccgacccc gaagttgggc ggcatcgacg ccgccttcag ccccgactgt
    10501 tgccacgcac cgtgcttgag gttggcgatg tactgcggca ggcgccgcat cgaggccatg
    10561 atcagcgccc aggtcagctc cgccggcgcg tacggcgcgc ccacgccctc ggcgacggcg
    10621 atgcccagct gcgtgcaggc cgcgacgtcg atgtgctcgc cgacgcggcc ggtctgggcg
    10681 atcagccgca gcttgggcag cttttccagc aactggcgcg ggaagcgcgt gcgctcgcgg
    10741 atcagcacca gcacctcggc ctcgcgcagc cgcacggaca gctggccgat gcccttgacg
    10801 gtgttggtga agaccttcgc gttgagctcg ctgagcttgg cggcgcatgg cagcttgcgc
    10861 accgcgtcct ggtagtcgtc gaggatgatg atgttcacgg ccgtcgatta tgactgcctg
    10921 ggctcgctcc gctcgccgcc cccgaggggc tcaatcggct cgctccgggc ggccgagcgc
    10981 tcgccgatgg gcggcgtttc ccttgacggg tcggttcgct tcgctcccga ctccgtgggc
    11041 ggacatatga cggcccgggc tcgctccgct cgccgccccc gaggggctca atcggctcgc
    11101 tccggagcgg ccgagcgctc gccgatgggc ggcgtttcgc ttgacgattc ggttcgcttc
    11161 gctcccgatt ccgtgggcgg ccgcttggac gaccaggggg cgaggtttca gcgccaggcg
    11221 gcggcgggtt gggcgctcat ccgggccgtg cggagggcgg cggggaaggt ggtgtccagc
    11281 gtgcggtaga gctcggcgcg gagcttgcgc gtgtcctcgc ggtcgtcctc gcgcagggcg
    11341 gcgagcagcg ggccgcgcag cagccacagg tcgaccggat cctccgcggc gcgcacctgc
    11401 gcgcgcagcc aggcgccctt gcggtgctcg aggttctcga ccgcgcgcag gaacagccgc
    11461 ttcatgccga cgaaggcgtg gcgggcggcg atgcgcgcgg tgcggtcggc cggcaggcct
    11521 gcatgccccg gggcgtcgaa ctgcgcggcc agggcgtcgg cctcggcgac gagggcgggg
    11581 tccagctcgg caagcccgag cgggtcgtag cggcggggtt caatggacat cggcggcttc
    11641 cggggcgggt tggacgatgc gctgggcgcg cgtcgggaag tgctggttca cgcgcgcgag
    11701 gcgggcctcg gcctcgacct ggctcacgcg gcgggcgatc atcgtgtaga gctccgagcg
    11761 caggtgccac agctcgcgca gcgagcgggc atgctgggcg cgacgcagca ggtcctcggt
    11821 gtccgcatcg agcagcccga ccatcgcctc gacgaactcg tggcgggcac gctccagcgg
    11881 cgtgcgcagc gcgggcacgg cctcgaggtc gttgccgacc agccagcgca gcgtcgcgtt
    11941 ccacagcgag ccgcggggcg cgcagaggtc cggcgggcag acgttcatgc gcagcgtcgt
    12001 gggcgcgacg ggcttgcgga ggatgcgggc gaagagggcg gtgctcatgt gatggatttc
    12061 ggcgccaggg ctccgaaaca tgagctgatt gttaaaaagt gcagcaattc gtaagcgcgg
    12121 aaaagacccg cgcttcccgc cagttcgcgc ccgtgccggc cgcccgcgca gcgtcgccgc
    12181 tggcagcacc gggcaggttc agcgcgccgg cccgggctgc ggctggccgt tcctggcccg
    12241 gtcgatggcg tcgcagagct cgcgggcgcc gtcgacgaag cgcggcccgc tgcgcgcgat
    12301 caggtcgccg tcgatgcgcg cgaattcccc gtgtcgggtg gcgctcacct gggggaagtc
    12361 cgcccaccgg ccgggcttcg cgggttccga cgggctgccg ccggtcacga ccagctcggg
    12421 gtcgcgctgc accgcggctt cccagctcac gctggcggtg agcgtcggca ggtcggcgaa
    12481 cacgttgcgg ccgccgcagg ccgcgatcgc actggagatg aggtgtgccc cgctcagcgt
    12541 catcaacggc gcgtcccaca gctggaagaa cacgcgcacg ggccgccggc cggcgtactc
    12601 gcgctgcagc gtcgtccagt cggccgcgat cgcccgcgcc cgggcatcgg ccacctgcgc
    12661 ggtgccggcc agcgtgccga ggcggcgcag cgccgactcc agcccgccga cgtcgcggat
    12721 ctcgatctcc atgaccggcc agccgagctc gcgcagcttg tccttgaagc gctggggcgt
    12781 gccgctgccc caggccagca cgaggtcggg ccgcagcttc gcgagggcct cgtagtcgac
    12841 cgcgaacgcg tccccgacga cgggcttggc gagcgcctcg cgcgggtagt cgctgtagcg
    12901 gccgaccgcg accaccgcgt cgccggcgcc ggccgcgtag gcgagctcgg tgaggtgcgg
    12961 cgcgagcgtg acgatacggc gcgcgggacg gtcgagccgg atctcgtggc ccgcgtcgtc
    13021 gacggccgtc gggcccgcgg cgtgcgccag gcc
//
LOCUS       DASVLN010000791         1661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_94697, whole genome shotgun sequence.
ACCESSION   DASVLN010000791 DASVLN010000000
VERSION     DASVLN010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94697"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VF453_18735"
     CDS             <1..1075
                     /locus_tag="VF453_18735"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7689749.1"
                     /translation="AATVPTPMPLTTSQQRALTWLALAAAAALLLRVLGTTLTPVVLA
                     LALGYLVWPLVVAIERRGAPRALAAGITIVVAMAAVTVLVMLLVPIVTTLLPMLEEQW
                     PAALDRFDRIVAPWLKGWGVQLDTSTVKDWLLSGNVNMEEWGGKLLASARVGGSWLLT
                     FAGVAVLVPVLAFYLLVDVERVGAGAWSLVPLKSRPGLAAFLAECDTTLRQYLRGQLA
                     VMGAMALFYPLALTVAGLNLAWPIGVFTGLAVFIPYLGYGTGLVLALMTAAIQFGSWQ
                     GPLGVAAAYALGQVLESFVLTPRLVGERIGLSPLAVILVLLASGEVFGFAGVLVALPA
                     SAVGVVVMRHLLAAWRASAFYRT"
     gene            1137..1658
                     /locus_tag="VF453_18740"
     CDS             1137..1658
                     /locus_tag="VF453_18740"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX7689750.1"
                     /translation="MRQLILDLAWAPLPPFAGFSACLSPLRRPEPTMAPTDAKASALY
                     ANTARALREAKARGFDNAVLLDAAGNVAEFATSNLFVVTAAGDVVTPALNGTFLAGIT
                     RARVIALLAGAGVTVTERTVRPEELDTAREIFSTGNYGKVTPCTRYESCELPAGPVAK
                     QARELYLAYTETT"
BASE COUNT          192 a          615 c          598 g          256 t
ORIGIN      
        1 cgcggccacc gtcccgacgc ccatgcccct cacgacgtcc cagcaacgcg ccctgacctg
       61 gctggccctg gccgccgcgg ccgcgctgct gctgcgcgtc ctggggacga cgctcacgcc
      121 ggtcgtgctc gcgctggcgc tcggctacct ggtctggccc ctggtcgtcg ccatcgagcg
      181 ccgcggcgcc ccgcgcgcgc tggcggcggg catcacgatc gtcgtcgcga tggccgccgt
      241 caccgtgctg gtgatgctgc tcgtgcccat cgtcacgacg ctgctgccga tgctcgagga
      301 gcagtggccg gcggcgctgg accggttcga ccgcatcgtc gcgccctggc tgaagggctg
      361 gggcgtgcag ctggacacct ccacggtcaa ggactggctg ctgagcggca acgtcaacat
      421 ggaggaatgg ggcggcaagc tgctcgcctc cgcgcgcgtg ggcggcagct ggctgctgac
      481 cttcgccggc gtcgcggtgc tggtgccggt gctcgcgttc tacctgctgg tcgacgtcga
      541 gcgcgtcggc gccggcgcct ggagcctggt gcccctgaag tcgcgcccgg ggctcgcggc
      601 cttcctcgcg gaatgcgaca cgaccctgcg ccagtacctg cgcggccagc tggccgtgat
      661 gggggcgatg gcgctgttct acccgctcgc gttgacggtg gcggggctga acctcgcctg
      721 gccgatcggc gtgttcaccg gcctggccgt cttcatcccc tacctcggct acggcaccgg
      781 cctcgtgctg gcgctgatga ccgcggcgat ccagttcggc agctggcagg ggccgctggg
      841 cgtcgcggcg gcctacgcgc tcgggcaggt gctcgagagc ttcgtcctga ccccgcgcct
      901 cgtgggcgag cgcatcgggc tgtcgccgct ggcggtgatc ctcgtcctgc tggcgtccgg
      961 cgaggtgttc ggcttcgccg gcgtgctggt cgcgctgccg gccagcgccg tcggtgtcgt
     1021 cgtgatgcgc cacctcctgg cggcctggcg cgcgagcgcg ttctaccgca catgagcacg
     1081 acggcccggc cgcccgcagg ggctcgcacc gcagctcgca cgacggaggt tcgccggtgc
     1141 ggcagctgat cctcgacctc gcctgggcgc cgctgccgcc gttcgccggc ttctccgcct
     1201 gcctgtcgcc gctgcgccgc ccggaaccga cgatggcgcc gacggacgcc aaggcgtccg
     1261 cgctgtacgc caacacggcg agagcgctgc gcgaggcgaa ggcgcgcggc ttcgacaacg
     1321 ccgtcctgct ggatgcggcc ggcaacgtgg cggagttcgc gacgtcgaac ctgttcgtcg
     1381 tgacggccgc cggcgacgtc gtcacaccgg cactgaacgg caccttcctg gccggcatca
     1441 cccgcgcccg cgtgatcgcg ctgctggccg gggcgggcgt gaccgtcacc gagcgcacgg
     1501 tcaggccgga agagctggac acggcccgcg aaatcttcag caccggcaac tacggcaagg
     1561 tcacgccgtg cacgcgctac gagtcgtgcg aactgcccgc ggggccggtg gccaaacagg
     1621 cgcgcgaact gtacctcgcc tacaccgaaa cgacttaagt c
//
LOCUS       DASVLN010000792         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_151538, whole genome shotgun sequence.
ACCESSION   DASVLN010000792 DASVLN010000000
VERSION     DASVLN010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151538"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VF453_18745"
     CDS             <1..503
                     /locus_tag="VF453_18745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX7689751.1"
                     /translation="WGDEPDVGLDAPAAAAPTSGVALPPPLPEFERTELGTRWYELVC
                     KCNEASSLVALVRELAMQAELIQCAADGDGGQTWTLRVARETLRNPTLVDKLAAAMRV
                     HVGAGLKLAVVAGEAQDSPTKRDAAEADRRQREAIAAVQSDPTVRALLAQFRTARILP
                     GSIKPL"
     gene            564..890
                     /locus_tag="VF453_18750"
     CDS             564..890
                     /locus_tag="VF453_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX7689752.1"
                     /translation="MMKNQLAGLMKQAQAMQDNLKKVQDELATIEVQGESGAGLVKIT
                     MTCKHDVRRVAIDPSLLADDKDMLEDLVAAAFNDALRRAETVSQEKMSKVTAGMPLPP
                     GMKMPF"
     gene            964..1551
                     /gene="recR"
                     /locus_tag="VF453_18755"
     CDS             964..1551
                     /gene="recR"
                     /locus_tag="VF453_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX7689753.1"
                     /translation="MSEARLVSLVEALKALPGVGQKSAQRMAFHLLQHDRDAALRLAT
                     ALEAAVHDIRHCARCHTFTEHELCDVCADPARDASQLCVVETPADQSALERTGSYRGL
                     YFVLMGRLSPLDGVGVRDIGVAPLLERVEREPVAEVILATSFTAEGDATAHVLGEALK
                     ARGLTVSRLARGVPAGSELEYVDLSTIAHALADRR"
BASE COUNT          263 a          676 c          672 g          234 t
ORIGIN      
        1 cctggggcga tgagcccgac gtcggcctcg acgcgcccgc ggccgcggcg ccgacctcgg
       61 gcgtcgccct gccgccgccg ctgcccgagt tcgagcgcac ggagctcggc acccgctggt
      121 acgagctggt ttgcaagtgc aacgaggcct ccagcctggt cgcgctggtg cgcgagctcg
      181 cgatgcaggc cgagttgatc cagtgcgcgg ccgacggcga cggtgggcag acctggacgc
      241 tgcgcgtcgc gcgcgagacg ctgcgcaacc cgacgctggt cgacaagctc gcggcggcga
      301 tgcgcgtcca cgtgggcgcg gggctgaagc tggcggtcgt cgccggcgag gcgcaggatt
      361 cgcccacgaa gcgcgatgcc gccgaggccg accgtcgcca gcgcgaggcg atcgcggccg
      421 tgcagtccga cccgaccgtg cgcgcgctgc tcgcgcagtt ccgcacggcg cgcatcctgc
      481 ccggctcgat caagccgctc tgatttgaag ctggggcgcc gcgcgccccg tcccgacaca
      541 cgaagccacg aaaggccctt gccatgatga agaaccaact cgccggcctg atgaagcagg
      601 cgcaggcgat gcaggacaac ctgaagaagg tgcaggacga gctcgccacg atcgaggtgc
      661 agggcgagtc cggcgccggc ctggtcaaga tcacgatgac ctgcaagcac gacgtgcgcc
      721 gcgtggccat cgacccgagc ctgctcgcgg acgacaagga catgctggag gatctcgtcg
      781 ccgccgcatt caacgacgcg ctgcgccgcg ccgagaccgt gagccaggag aagatgtcca
      841 aggtcaccgc gggcatgccg ctgccccccg gcatgaagat gcccttctga gcggccgcat
      901 gagcctacgt ccggccgctc cgaaggtggc tcgcatcgca gtgcgcagcg cggggctcgc
      961 gcggtgagcg aggcccgcct ggtctcgctc gtcgaggcgc tgaaggcgct gcccggggtc
     1021 gggcagaagt cggcgcagcg catggccttc cacctgctgc agcatgaccg cgacgcggcg
     1081 ctgcggctgg ccacggcgct cgaagccgcc gtgcacgaca tccgccactg cgcgcgctgc
     1141 cacaccttca cggagcacga gctgtgcgac gtctgcgccg acccggcgcg cgacgcgtct
     1201 cagctgtgcg tcgtcgagac gccggccgac cagtccgccc tcgagcgcac cggcagctac
     1261 cggggcctgt acttcgtgct gatggggcgg ttgagtccgc tggatggcgt cggcgtgcgc
     1321 gacatcggcg tcgcgccgct gctggagcgc gtcgagcgcg aacccgtggc cgaggtcatc
     1381 ctggccacca gcttcacggc cgagggtgac gccacggccc acgtcctcgg cgaggccctg
     1441 aaggcgcgcg gcctcaccgt ctcccgcctg gctcgaggcg tgccggcggg cagcgaactg
     1501 gagtacgtcg acctgtcgac gatcgcgcac gcgctggccg accggcgctg atcaggggcc
     1561 cggcgggtcg cgacgatccg gctgcgacgg tggcgctcca acccagcccg ccgtgctgcg
     1621 ccgcagtcag gtggctgcat ccgccgccgg cccggccaac gaaaacggcc gcacgcggcg
     1681 gccgtccgga cgaagcgcaa gcgccgttca gcgctgcgcc accttcgact tgcccttctc
     1741 gagccgcgga gatgccgcgc cgccggcggt cgccttggcg tgggcgacga cgacggcctt
     1801 gccgccgcgg tgcggggcct tcgagacggc cacgcgcggg ccgct
//
LOCUS       DASVLN010000793        11428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_526372, whole genome shotgun sequence.
ACCESSION   DASVLN010000793 DASVLN010000000
VERSION     DASVLN010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526372"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VF453_18760"
     CDS             <1..1034
                     /locus_tag="VF453_18760"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7689754.1"
                     /translation="AQLADFEPAAWCAAAARWLHGRRAELAPVFAARRALGAVRDGHG
                     DLHLSNTVVLGDDVTAFDCIEFDPALRCIDVIADIAFMTMDLHAHGRPDLAHRFLDAY
                     LAASGDYEGLALLRFHEAARACVRGLVQRLGPPADRAGAPDYLALATRRMTEGDARPR
                     LAILCGPSGAGKSTIAARLVDLTGAVRVRSDVERKRLHATPTAAEEDASALYSHAATE
                     RTYGRLADCARAVMRAGFDVIVDAAFLDRARRDAFAALAAELGVAFVVLSFDAPPQEL
                     ARRVARRAAAGGDPSDADEAVLAGQLRSRDPIAPRERARTIRVDTTQAVDVGRLAASW
                     HAHPSSIAG"
     gene            1101..2588
                     /locus_tag="VF453_18765"
     CDS             1101..2588
                     /locus_tag="VF453_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HEX7689755.1"
                     /translation="MLDFFARRLWRERTRHRHPAQGVLLAVLLAAVFPASAIPVFNRQ
                     TGQNCQACHAGGQFPELTPYGRLFKLTGYTIGARPAVPLSAMAVASVSSVRDTHKSDD
                     PAADFPKNDDLLFSSASLFAGGRITDNVGAFLQYTYDPYANRDDSGRFHGHAAADNMD
                     IRYADRFVGAHDLVVGVDVNNNPSVSDPWNTAAAWMQYVPVPSPGSSQFIDGATPYPG
                     YAVGGNTSGLTAYAFLDERLYVEAGAYRTTRGPFAFMKLGQPTAALTRLQGNAPYVRA
                     AYNLQWGASALEFGASTMSSRIYDDPANPGDPATLHRFRDASLDAQYQWLLDPHAATV
                     QLVLTRQHHRYPAFLAGQASPFVDAQGNPLAPSSPSDTLRLVRAKAAYTWRATYGASL
                     SYFDLSGTTNTLNQSSGYDPSTGTITSSPSAQAPSTRVNGNASGNPSTVGETLEAYWL
                     PWQNLRIGLQFTAYQRYNGSARNYDGFGRNASDNDSLFLYAWLAY"
     gene            2602..3237
                     /locus_tag="VF453_18770"
     CDS             2602..3237
                     /locus_tag="VF453_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7689756.1"
                     /translation="MNALCSIVVLSAACLAGGCAQPSRSRALGDARVPAQALAEQVCS
                     NCHGPRGLAVSDNFPHLAAQQPEYLEGQLRSLRERHRRDPGGYVYMWGIARGLSDAQI
                     TGLAAYYAAQPAPVARPAGLVFPREGEALFKLGAPDRGVPACRTCHGDNGQGQGAFPR
                     LAHQHAPYVVRQLEILREGGDNRPLGAVMNPVAHGLSDDDIRAVAAYIEGM"
     gene            complement(3251..4867)
                     /gene="groL"
                     /locus_tag="VF453_18775"
     CDS             complement(3251..4867)
                     /gene="groL"
                     /locus_tag="VF453_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092705.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7689757.1"
                     /translation="MSPKQLAFGDGARHRLLGGIDLLAEAVKVTLGPRGRTVILDDAD
                     GHPRVVNSGVVVARSIELADRFEDMGAQLLRVAASRTSELAGDGTTTATVLAHAMIRE
                     GVKHIAAGMNPMELKRGLDRAVDAVAGRLAAQAQACADGAQVSHVATISANNDHVIGD
                     LVARAFDRVGREGAISIEDGSGVASALEVVDGMRLDRGYVSAYFVNDTARQRVVLDDA
                     RILVCDHRLASAQAIVPILEEVARAGAALLLVADDFDEDVVATLVVNGVRGVLKVCAV
                     KAPGFGDARKAALADIALATGATLVSAELGRSMDRITLEQLGRVRRVTVEKDSTTLAG
                     SGGDPPGLAARVAALRRERDETADAHAKRALDQRIGRLTGGVCVIKVGATTELELKER
                     KLRVEDALHATRAAIEEGIVPGGGVALLQGRRVLNELQAETLEQRAAIAIVDRALQEP
                     LRCIVENAAGDPATVVARIEDAQSHAYGWNALTRAYGNLLDMGVIDPVKVARLALQNA
                     ASVAGLVLATDCVITQAPAVAPVEAEALSL"
     gene            5059..5502
                     /locus_tag="VF453_18780"
     CDS             5059..5502
                     /locus_tag="VF453_18780"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7689758.1"
                     /translation="MNPAIASRPNLGRSAADSIADLFRRVARMADGASPVGDDMRVDI
                     SETDHAYLVKAELPGVAKEDIRIRIDGSYVSISTTVRRETGRARKHEEERVLLHETHD
                     GTWSRGLSLPHELADDGAQASLDNGVLSLTLPKRTPAAGKAIAIK"
     gene            5548..6204
                     /locus_tag="VF453_18785"
     CDS             5548..6204
                     /locus_tag="VF453_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7689759.1"
                     /translation="MQPPVQRIDVGAVALEADLSLPERARGLVVFAHASGSARRSPRN
                     RLFARALQQQGLATLVFDLLLPQESTDRGKSFDIELLSRRLEQVIAWIDGHPKLAMLP
                     LALLAAGTGAAAALVVAARLPHRIGAIVSRGGRPDLAGPALAHVGAPTLLIVAADDVH
                     ALAQNREALGQLHAQAQLSLVGPGADSFEDPGDLDRAAALAARWLVAHLPPVGQVVGR
                     "
     gene            complement(6212..6667)
                     /locus_tag="VF453_18790"
     CDS             complement(6212..6667)
                     /locus_tag="VF453_18790"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7689760.1"
                     /translation="MYSRILVPIDGSEPSQCGLREAIAIARQNGSTLVLLHVVTQLPM
                     PIGDPGAFSYRQVWDMLEHAGREIVEKSAKEAAAAGVANEQRLVDGGAGPVCDVVVAQ
                     AAAERCELIVMGTHGRRGMKRLAMGSDAELVVRHAGVPVLLVKPRDFAA"
     gene            complement(6787..7920)
                     /locus_tag="VF453_18795"
     CDS             complement(6787..7920)
                     /locus_tag="VF453_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7689761.1"
                     /translation="MLQRILQLLIKEFLQLRRDRSARFRLLVPPVIQILLFGYAATFE
                     VFNVSTIVADQDRSPESRALVAAFVHSSRFEVTAIAASRQDVQAAIQRSDVHVGIVVP
                     AGFSRELRAGRDAPIQVLLDGTNSNTALIAGGYVGQIAQSFGEAQAGALLQASGQALV
                     APRVRFPLGQRFWFNPNLDSRWFFVPGVIGSLVLVTIVNLTAFALVREREVGTLEQLL
                     VTPIRPVEFILGKTLPFFLIGAMEVAIVAGIGLLWFHVPFNGSALLLAGGTALFLLSA
                     LGIGLLISTVCRTQQQAFASNFFVLNPMFILSGFGFPIASMPQPLQWLTLVDPLRHFL
                     VIIRGTFLKGVGLEVLWPEFTALAVLGALLLSASVLRFRKSLD"
     gene            complement(7927..9063)
                     /locus_tag="VF453_18800"
     CDS             complement(7927..9063)
                     /locus_tag="VF453_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7689762.1"
                     /translation="MNRRRMIAVARKEWLQVVRDSRSLMIALLLPLMQMFLLGYGVKL
                     DVSDIPLCVVDEDGSQRARELVERFAASRYFALRAVDHTVASATRRIDDGTCRLALVL
                     PPRLSAKLDGEHATTLQLLVDGTDSNTATIAAGYARSVVDAHAAALQLQALQRHGLPT
                     HAAIPLQARPRVWFNEDLESSHFIIPGVVALVMAVIGSQLSSLTIAREWERGTMELLV
                     STPVSPLELMVGKLVPYFAIGMLDAAICVAVAVGWFGVPFRGALATLVGTTGLYLAVV
                     LSIGYLVSVRIRSQVGASQVALVLTMLPTTLLSGFTFPIDQMPRAVQAVTSLVSARYY
                     VTIVKSIFLKGSGAAELAVPIACLALYAAVVIALAARSFRKTLD"
     gene            complement(9060..10028)
                     /locus_tag="VF453_18805"
     CDS             complement(9060..10028)
                     /locus_tag="VF453_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689763.1"
                     /translation="MTAAPGSGPAIVVQRLGKRFGDFEALADVSFDVGRGEVMGFLGP
                     NGAGKSTLIRILCGLLRPGSGSAHVVGIDVARDPEAVRQRIGYMSQRFSLYDDLSVAE
                     NLRFFGRVYGVSAKDIVERTRAAVAMAGLDGREDVPAGMLAGGWKQRLALGCAILHRP
                     QVLFLDEPTSGVDPGSRRRFWGLIHDLASDGVSILVSTHYMDEAEYCTRVALLAAGRL
                     IALGAPHELKQRAAGGTVMEISCGDTARALDIATTVEGMRDAAVFGNTVHVTALHAGA
                     GATLLAALRSAGLADARLRPIEPSMEDMFVHLVQASRTPAPAGSAA"
     gene            complement(10025..10975)
                     /locus_tag="VF453_18810"
     CDS             complement(10025..10975)
                     /locus_tag="VF453_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689764.1"
                     /translation="MTTPGVAEPAVQAQGLARRFGRTQAVASLDLQVARGEIFGLVGP
                     DGAGKTTTMRMLAGVMRPDAGRIVIDGVDVVAQPEQARHRVSYMPQRFALYEDLTVDE
                     NIRFFADLFGVPDAVRRERAARLLAASAMTPFRSRLAGQLSGGMKQKLGLTCALVHAP
                     RVLLLDEPTTGVDPVSRREFWQILYGLRAEGVAMVLSTAYLDEAERCDRLALLHDGVV
                     MHAGTPAHLKTLLPGAILRVATAGPRAVAVLARACEGVIGVLPVGDGVNLHVDDAALR
                     RPQLAAALAPAGVRAGDIAPVEPSIEDLFVALLDRAPAAP"
     gene            complement(10972..>11428)
                     /locus_tag="VF453_18815"
     CDS             complement(10972..>11428)
                     /locus_tag="VF453_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7689765.1"
                     /translation="NIATFEASGRGAREAARLARIQAGNATLVAPFDGVITARQAELG
                     EVVAPGTPVVTLADLDHVWVRAYINETDRLRVRLGQRVVVTSDAPRDGRVDGRLSFVA
                     SEAEFTPKSVETHAERVTLVYRVKVDIDNRGHAFLPGMPVDVRLAAAAP"
BASE COUNT         1618 a         4203 c         4123 g         1484 t
ORIGIN      
        1 tggcgcagct cgccgacttc gagcccgccg cctggtgcgc ggccgcggcg cgatggctcc
       61 atggccggcg cgccgagctc gccccggtct tcgccgctcg ccgcgcgctc ggggccgtcc
      121 gcgacggtca cggggatctc cacctgtcca acaccgtcgt gctcggcgac gacgtgaccg
      181 ccttcgactg catcgagttc gaccccgcgc tgcggtgcat cgacgtcatc gccgacatcg
      241 cgttcatgac gatggacctg cacgcgcacg ggcgaccgga cctggcgcac cgcttcctcg
      301 acgcctacct ggccgcgagc ggcgactacg agggactcgc gctgctgcgc ttccacgagg
      361 cggcgcgggc ctgcgttcgc ggcctggtgc agcgcctcgg gccgcccgcc gaccgcgcgg
      421 gcgccccgga ctacctggcg ctggcgacgc gtcgcatgac cgaaggcgac gcgcggccga
      481 ggctcgcgat cctgtgcggc ccgtcgggcg ccggcaagtc gacgatcgcg gccaggctgg
      541 tcgacctgac cggcgccgtg cgcgtgcgat cggacgtgga gcgcaagcgc ctgcacgcga
      601 cgcccacagc tgccgaggag gatgcgtccg cgctctattc gcacgcggcg acggagcgca
      661 cctatgggcg ccttgccgac tgcgcacggg cggtgatgcg cgcgggcttc gacgtcatcg
      721 tggacgccgc gttcctcgat cgggcccggc gcgacgcgtt cgcggccctg gcagcggagc
      781 tgggcgtcgc gttcgtcgtg ctctcgttcg acgcgccgcc gcaggagctg gcacgccggg
      841 tcgcccgccg cgccgccgcc gggggcgacc cgtcggacgc ggacgaggcg gtgctcgccg
      901 gccagcttcg atcgcgcgat ccgatcgcgc cgcgcgagcg cgcccgcacg atccgggtcg
      961 acacgacgca ggccgtcgac gtcgggaggc ttgcggcaag ctggcatgcg catccgtcgt
     1021 cgatcgccgg ttgacgcagc gcaacgggtg ccggccgcga cggggctgga atgcgggcag
     1081 gattcgacgg aggttcggtc atgctcgact tcttcgcgag acggctctgg cgcgagcgca
     1141 cgcggcatcg ccacccggcg cagggcgtcc tgctggccgt cctcctcgcg gccgtcttcc
     1201 cggcgtcggc gatccccgtc ttcaaccgcc agacggggca gaactgccag gcgtgccacg
     1261 cgggcgggca gttccccgag ctgacgccct acggacgcct gttcaagctc accggctaca
     1321 cgatcggcgc gcgtccggcg gtgccgctct cggcgatggc cgtcgcgagc gtctcctcgg
     1381 tgcgcgacac gcacaagagc gacgacccgg ccgccgactt cccgaagaac gacgacctgc
     1441 tgttctcttc ggcgagcctg ttcgccggcg gccgcatcac cgacaacgtc ggcgccttcc
     1501 tgcaatacac ctacgacccg tacgcgaacc gggacgattc cggccgcttc cacggccatg
     1561 cggcggcgga caacatggac atccgctacg ccgaccggtt cgtcggggcg cacgacctcg
     1621 tcgtcggcgt cgatgtcaac aacaatccct cggtgtcgga cccgtggaac acggccgccg
     1681 cgtggatgca gtacgtgccg gtgcccagcc cgggcagcag ccagttcatc gacggcgcca
     1741 cgccgtatcc gggctacgcc gtcggcggca acacctccgg cctcacggcc tacgcgttcc
     1801 tcgacgagcg cctctacgtc gaggcgggcg cgtaccgcac gacccgcggc ccgttcgcct
     1861 tcatgaagct gggacaaccc accgcggcgc tcacgcgcct gcagggcaac gcgccctacg
     1921 tgcgcgccgc gtacaacctg caatggggcg ccagcgcgct cgagttcggg gcgtcgacca
     1981 tgagctcgcg catctacgac gatcctgcca atcccggcga tcccgccacg ctgcatcggt
     2041 tccgcgacgc ctcgctcgac gcgcagtacc agtggctgct cgacccgcac gcggccacgg
     2101 tgcagctcgt cctcacccga cagcaccatc gctacccggc cttcctcgcg gggcaggcgt
     2161 cgccgttcgt ggatgcgcag ggcaacccgc tggcgccgag ctcgccgtcg gacacgctca
     2221 ggctcgtccg ggccaaggcc gcgtacacct ggcgcgcgac gtacggggcg agcctctcct
     2281 atttcgatct ctcgggaacg acgaacacgc tgaaccagag ctcgggctac gacccgtcga
     2341 ccgggacgat cacgtcgtcg ccttcggcgc aggcgccgtc cacgcgcgtc aacggcaacg
     2401 cgtcgggcaa cccgtccacg gtgggcgaga cgctggaagc ctactggctg ccgtggcaga
     2461 acctgcgcat cggcctgcag ttcacggcct accagcgcta caacggcagc gcgcgcaact
     2521 acgacggctt cgggcgcaac gcgtcggaca acgactcgct cttcctctac gcgtggctcg
     2581 cctactgacg ggagaagcaa gatgaacgcc ctctgttcga tcgtcgtgct gtcggccgcc
     2641 tgcctggccg gtgggtgcgc gcaaccctcg cgctcgcgcg ccctcggcga cgcgcgcgtg
     2701 cccgcgcagg cgctggcgga gcaggtctgc tcgaactgcc acgggccgcg cggcctcgcg
     2761 gtctcggaca acttccccca cctcgccgcg cagcagcccg agtacctcga gggccagctg
     2821 cgcagcctgc gcgagcggca tcggcgcgac cccggcggct acgtctacat gtggggcatc
     2881 gcgcgcgggc tgagcgatgc gcagatcacg ggactcgcgg cctactacgc ggcgcagccc
     2941 gcgccggtcg cgcgccccgc ggggctggtc tttccgcgcg aaggcgaggc gctgttcaag
     3001 ctcggcgcgc cggaccgcgg cgtgcccgcg tgtcggacct gccatggcga caacgggcag
     3061 ggccagggcg cgttcccgcg gctggcgcac cagcacgcgc cctacgtcgt gcgccagctc
     3121 gagatcctgc gcgaaggggg cgacaaccgc ccgctgggcg cggtcatgaa ccccgtggcg
     3181 cacggcctga gcgacgacga catccgcgcc gtcgccgcct acatcgaagg catgtgaggc
     3241 cgcgcggcgc ctagagcgac agcgcctccg cctcgaccgg cgcgaccgcc ggcgcctgcg
     3301 tgatgacgca gtccgtcgcc agcacgaggc cggcgaccga ggccgcgttc tgcagcgcga
     3361 gccgcgcgac cttcaccggg tcgatgacgc ccatgtccag gaggttgccg taggcccgcg
     3421 tcagggcgtt ccagccgtag gcgtgcgact gcgcgtcctc gatgcgcgcg acgacggtcg
     3481 ccgggtcgcc ggccgcgttc tccacgatgc agcgcaaggg ctcctgcagc gcgcggtcga
     3541 cgatggcgat ggccgcgcgc tgctcgagcg tctcggcctg cagctcgttc agcacgcgcc
     3601 ggccctgcag cagggccacg ccgccgcccg ggacgatgcc ttcctcgatc gcggcgcgcg
     3661 tcgcgtgcag ggcgtcctcg acgcgcagct tgcgttcctt gagctccagc tccgtggtcg
     3721 cgcccacctt gatgacgcag acgccgccgg tcaggcgccc gatgcgctgg tcgagcgcgc
     3781 gcttcgcatg cgcatccgcc gtctcgtcgc gttcgcggcg cagggccgcg acgcgcgcgg
     3841 ccagtcccgg cggatcgccg ccgctgccgg ccagggtcgt cgagtccttc tcgaccgtca
     3901 cgcggcgcac gcggcccagc tgctcaaggg tgatccgatc catgctgcgc ccgagctcgg
     3961 ccgacacgag cgtcgccccg gtggccagcg cgatgtcggc cagcgccgcc ttgcgcgcgt
     4021 cgccgaagcc gggggccttc acggcgcaga ccttcaggac cccgcggacg ccgttgacca
     4081 ccagcgtggc gacgacgtcc tcgtcgaagt cgtccgcgac cagcagcagc gccgcgcccg
     4141 cgcgcgcgac ctcctcgagg atcgggacga tcgcctgcgc cgaggccagg cgatggtcgc
     4201 agacgaggat gcgcgcgtcg tccaggacca cgcgctggcg cgccgtgtcg ttgacgaagt
     4261 aggccgagac gtagccgcgg tccaggcgca tcccgtcgac gacctcgagc gcgctggcga
     4321 cgcccgagcc atcctcgatc gagatcgcac cctcgcgacc cacgcggtcg aacgcgcgcg
     4381 cgaccaggtc gccgatgaca tggtcgttgt tggccgagat cgtcgcgacg tgcgagacct
     4441 gcgcgccgtc ggcacaggcc tgcgcctgcg cggcgaggcg gcccgcgacg gcgtcgacgg
     4501 cgcggtcgag gccgcgcttg agctccatcg ggttcatccc cgcggcgatg tgcttcacgc
     4561 cctcgcggat catcgcgtgc gcgaggacgg tggcggtggt cgtgccgtcg cccgcgagct
     4621 cgctggtgcg cgaggccgcg acgcgcagca gctgcgcgcc catgtcctcg aagcggtcgg
     4681 cgagctcgat cgagcgcgcg acgaccacgc ccgagttgac cacccgcggg tgcccgtccg
     4741 cgtcgtcgag gatcaccgtg cggccgcgcg gtccgagggt gaccttgacg gcctcggcga
     4801 gcaggtcgat gccgcccagg aggcgatggc gcgccccgtc gccgaacgcc agctgcttgg
     4861 gactcatggg agggcctcgt cgagtgggat ctgcatcgac ggtagggcgg cgcgggcggc
     4921 gccgcgttga gtgcgcgcaa tcgcggcggc gggcgccgga cgacgccgga ttgcgtccgc
     4981 gcaagtcgcc ggcgcatggc ggatccacca tgcgggcgtc gcgccaggaa ggcgcttcca
     5041 tccccgcagg agacccgaat gaaccccgcc attgcttctc gtccgaacct gggccgatcg
     5101 gccgccgatt cgatcgccga cctcttccgc cgcgtcgccc ggatggccga tggcgcgtcg
     5161 ccggtcggag acgacatgcg cgtggacatc agcgagaccg atcatgcgta tctcgtcaag
     5221 gccgagctgc cgggcgtcgc caaggaggac atccgcatcc gcatcgacgg cagctacgtc
     5281 tcgatctcga ccaccgtccg gcgcgagacc ggccgggcac gcaagcacga ggaggagcgc
     5341 gttctcctgc acgagacgca cgacggcacc tggtcgcgcg ggctgtcgct gccgcacgag
     5401 ctggccgacg acggcgcgca ggccagcctg gacaacggcg tgctgtccct gacgctgccc
     5461 aagcgcacgc cggcggccgg caaggccatc gcgatcaagt gaggcgggcg cgccggcggc
     5521 agccgccggc gcggacaaag cccgaggatg cagccgccgg tgcagcgcat cgacgtcggc
     5581 gccgtggcgc tggaggccga cctgagcctg ccggagcgcg cgcgcgggct ggtcgtcttc
     5641 gcgcatgcga gcggctcggc gcggcgcagt ccccgcaacc gcctgttcgc gcgcgcgttg
     5701 cagcaacagg gcctcgccac gctggtcttc gacctgctgc tgccgcagga gtccaccgac
     5761 cggggcaagt cgttcgacat cgagttgctg agccggcgcc tcgagcaggt catcgcgtgg
     5821 atcgacggcc atccgaagtt ggcgatgctg ccgctggcct tgctggcggc ggggacgggg
     5881 gccgccgcgg cgctggtcgt cgccgcgcgg ctgccccacc ggatcggggc gatcgtgtcg
     5941 cgcggagggc gtcccgacct cgccgggccc gcattggcgc acgtgggggc gccgaccctg
     6001 ctgatcgtcg cggcggatga cgtgcacgcg ctcgcgcaga accgcgaggc gctcgggcag
     6061 ctccatgcgc aggcgcagct cagcctggtc gggccgggcg cggactcgtt cgaggatccc
     6121 ggcgacctcg accgcgcggc cgcgctcgcg gcgcgctggc tggtcgcgca cctgccgccg
     6181 gtggggcagg tcgtcggccg ctgaagccgc gtcaggcggc gaagtcgcgc ggcttcacca
     6241 gcaggaccgg cacgccggcg tggcgcacga cgagctccgc gtcgctgccc atcgccaggc
     6301 gcttcatccc gcggcggccg tgcgtgccca tcacgatcag ctcgcagcgc tccgcggccg
     6361 cctgcgccac gaccacgtcg cacaccggcc cggcgccgcc gtcgacgagg cgctgctcgt
     6421 tcgcgacgcc ggccgcggcg gcttccttgg cggacttctc gacgatctcg cgcccggcgt
     6481 gctcgagcat gtcccagacc tgcctgtagc tgaacgcgcc cgggtcgccg atcggcatcg
     6541 gcagctgcgt gacgacgtgc agcagcacga gcgtcgaccc gttctgcctg gcgatcgcga
     6601 tggcctcccg caggccgcac tgcgacggct cgctgccgtc gatcgggacg aggatgcgcg
     6661 aatacatgct ggactcccgt gccgatcgac gcggcatcgg aagcggcgcg cgcacggcag
     6721 gatcattctg tcggcgcggc gcgcgcgccg cttgcgccgg cgcaatcgcc cgcgtggaag
     6781 gcggcctcag tccagcgact tgcgaaagcg cagcacggac gccgacagca gcagggcgcc
     6841 cagcacggcc agcgccgtga actcgggcca cagcacctcc agcccgacgc ccttgaggaa
     6901 ggtgccgcgg atgatgacca ggaagtgccg cagcggatcg accagcgtga gccactgcag
     6961 cggctgcggc atgctcgcga tcgggaagcc gaagcccgag aggatgaaca tcgggttgag
     7021 cacgaagaag ttggacgcga acgcctgctg ctgcgtccgg cacaccgtgg agatcagcag
     7081 cccgatgccc agggcgctga gcaggaacag ggcggttccg ccggccagca gcagcgcgct
     7141 gccgttgaac ggcacgtgga accacagcag gccgatcccc gcgacgatcg cgacctccat
     7201 cgcgccgatc aggaagaacg gcagcgtctt gccgaggatg aactccaccg gccggatcgg
     7261 cgtgaccagc agctgctcca gggtgccgac ctcgcgctcg cgcacgagcg cgaaggcggt
     7321 caggttgacg atggtgacca gcaccagcga gccgatgacg ccggggacga agaaccaccg
     7381 gctgtcgagg ttggggttga accagaagcg ctgcccgagc gggaagcgca cccgcggggc
     7441 gaccagcgcc tgcccgctgg cctgcagcag cgcgcccgcc tgcgcttcgc cgaacgactg
     7501 cgcgatctgg ccgacgtagc cgccggcgat gagcgcggtg ttggagttgg tgccgtccag
     7561 gagcacctgg atcggcgcgt ccctgcccgc gcgcagctcg cgcgagaagc cggccggcac
     7621 gacgatgccc acgtgcacgt cgctgcgctg gatcgccgcc tgcacgtcct gccgggacgc
     7681 ggcgatggcg gtcacctcga accggctgct gtggacgaac gccgcgacga gggcgcggct
     7741 ttccgggctg cgatcctggt cggcgacgat cgtcgagacg ttgaacacct cgaaggtggc
     7801 ggcgtagccg aacagcagga tctggatcac cggcggcacg agcagccgga agcgcgccga
     7861 ccggtcgcgc cgcagctgca ggaattcctt gatcaggagc tggaggatgc gttgaagcat
     7921 ggcgcctcaa tcgagggtct tgcgaaacga gcgcgccgcc agcgcgatca ccacggcggc
     7981 gtacagcgcg aggcaggcga tcggcaccgc gagttccgcc gcgcccgagc ccttgaggaa
     8041 gatggacttc acgatcgtga cgtagtagcg cgcgctcacg aggctcgtga cggcctgtac
     8101 cgcgcgcggc atctggtcga tcgggaacgt gaagcccgac agcagcgtcg tgggcagcat
     8161 cgtcagcacg agcgcgacct ggctcgcgcc gacctggctg cggatgcgca ccgagacgag
     8221 gtagccgatg gacagcacca cggccaggta gaggcccgtg gttccgacga gcgtcgccag
     8281 ggcgccgcgg aacgggacgc cgaaccatcc gaccgccacc gcgacgcaga tcgcggcgtc
     8341 gagcatgccg atcgcgaagt agggcaccag cttgccgacc atcagctcga gcggcgacac
     8401 cggcgtcgag acgagcagct ccatcgtgcc gcgctcccac tcgcgggcga tcgtcagcga
     8461 gctgagctgc gagccgatga cggccatgac cagcgcgacg acgccgggaa tgatgaaatg
     8521 gctgctctcg aggtcctcgt tgaaccacac gcgcggcctc gcctgcagcg ggatcgcggc
     8581 gtgcgtcggc agcccgtggc gctgcagcgc ctgcagctgc agggcggcgg catgcgcgtc
     8641 gacgacgctg cgcgcgtagc ccgccgcgat ggtggcggtg ttggaatccg tgccgtcgac
     8701 cagcagctgc agggtcgtcg cgtgctcgcc gtccagcttc gccgacagcc gcggaggcag
     8761 caccagggcg agccggcagg tgccgtcgtc gatgcgacgc gtcgccgagg ccacggtgtg
     8821 gtcgaccgcg cgcagggcga agtagcgcga cgccgcgaag cgttcgacga gctcgcgcgc
     8881 gcgctggctg ccgtcctcgt ccaccacgca cagcgggatg tcgctgacgt cgagcttgac
     8941 gccgtacccg agcaggaaca tctgcatcag cggcagcagc agcgcgatca tcaggctgcg
     9001 cgagtcgcgc accacctgca gccactcctt gcgggcgacg gcgatcatcc ggcgccggtt
     9061 catgccgcgc tcccggcggg cgcgggcgtg cgcgacgcct gcaccaggtg gacgaacatg
     9121 tcctccatcg acggctcgat cgggcgcagc cgcgcgtccg cgaggccggc cgagcgcaac
     9181 gccgccagca aggtcgcgcc ggcgccggcg tgcagggcgg tgacgtgcac ggtgttgccg
     9241 aacaccgccg cgtcgcgcat gccctcgacc gtcgtcgcga tgtcgagcgc gcgcgccgtg
     9301 tcgccgcagg agatctccat caccgtgccc ccggccgcgc gctgcttgag ctcgtgcggg
     9361 gcgcccagcg cgatcaggcg gccggcggcg agcagggcga cgcgcgtgca gtattcggcc
     9421 tcgtccatgt agtgcgtgga gacgaggatg ctcacgccgt ccgacgcgag gtcgtggatg
     9481 aggccccaga agcgccgccg cgagccggga tcgacgccgg aggtcggctc gtccaggaac
     9541 aggacctgcg gccggtgcag gatcgcgcat ccgagggcga gccgttgctt ccagccgccg
     9601 gccagcatgc cggcgggcac gtcctcccgc ccgtcgagcc cggccatggc gacggccgcg
     9661 cgcgtgcgtt cgacgatgtc cttcgccgag acgccgtaca cccggccgaa gaagcgcagg
     9721 ttctccgcga cgctgaggtc gtcgtagagc gagaagcgct gggacatgta gccgatgcgc
     9781 tggcgcacgg cctccggatc gcgcgccacg tcgatgccca ccacgtgcgc actgccgctg
     9841 ccgggccgca gcaggccgca gaggatgcgg atgagcgtgg acttgcccgc gccgttgggg
     9901 ccaaggaagc ccatgacctc gccgcgcccc acgtcgaagc tgacgtccgc cagcgcctcg
     9961 aagtcgccga agcgcttgcc cagccgctgc acgacgatcg cggggccgga gcctggcgcc
    10021 gccgtcatgg ggccgcgggc gcccggtcga ggagcgcgac gaacaggtct tcgatgctcg
    10081 gctcgacggg cgcgatgtcg ccggcgcgca cgcccgcggg agcaagcgcg gccgcgagtt
    10141 gcgggcgccg cagcgccgcg tcgtcgacgt gcaggttcac gccgtcgccc acgggcagca
    10201 ccccgatcac gccctcgcac gcgcgtgcca gcacggcgac cgcgcgcggg ccggcggtcg
    10261 ccacgcgcag gatggcgccg ggcagcagcg tcttgaggtg cgccggcgtg cccgcatgca
    10321 tgacgacgcc gtcatggagc agggcgaggc ggtcgcagcg ctcggcctcg tcgaggtagg
    10381 cggtcgagag caccatcgcg acgccctccg cgcgcaggcc gtagaggatc tgccagaact
    10441 cgcggcgcga caccgggtcg acgcccgtcg tcggctcgtc cagcagcagc acgcgcggcg
    10501 cgtgcacgag cgcgcaggtc agtccgagct tctgcttcat cccgccggac agctggccgg
    10561 ccaggcgcga gcggaacggc gtcatcgcgg aggcggcgag caggcgcgcc gcgcgctcgc
    10621 ggcggaccgc gtccggcacc ccgaagaggt cggcgaagaa gcggatgttc tcgtcgacgg
    10681 tcaggtcctc gtacagcgcg aagcgttgcg gcatgtagct cacgcgatgg cgtgcctgct
    10741 ccggctgcgc gacgacgtcc accccgtcga tgacgatgcg ccccgcgtcc ggccgcatca
    10801 cgcccgcgag catgcgcatc gtcgtcgtct tgcccgcgcc gtcggggccg accagtccga
    10861 agatctcgcc gcgcgccacc tggaggtcga gcgacgcgac ggcctgcgtc cggccgaagc
    10921 gccgcgccag cccctgcgcc tgcacggccg gctccgccac gccaggcgtc gtcatggcgc
    10981 cgccgccgcg agcctgacgt ccaccggcat gcccggcagg aacgcatggc cgcgattgtc
    11041 gatgtcgacc ttgacgcgat acacgagcgt cacgcgctcg gcatgcgtct cgacgctctt
    11101 gggagtgaac tcggcctcgg aggcgacgaa ggacaggcgc ccgtcgaccc ggccgtcgcg
    11161 cggcgcgtcg ctcgtcacga cgacgcgctg cccgaggcgg acgcgcaggc ggtccgtctc
    11221 gttgatgtac gcgcgcaccc agacgtggtc gaggtcggcg agcgtgacga cgggcgtgcc
    11281 cggcgccacg acttcgccga gctcggcctg ccgcgccgtg atcacgccgt cgaagggcgc
    11341 gacgagggtc gcgttgccgg cctggatgcg cgcgaggcgg gccgcttcgc gcgcgccgcg
    11401 gccgctggcc tcgaaggtcg cgatgttg
//
LOCUS       DASVLN010000794         2142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_202076, whole genome shotgun sequence.
ACCESSION   DASVLN010000794 DASVLN010000000
VERSION     DASVLN010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202076"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VF453_18820"
     CDS             <1..625
                     /locus_tag="VF453_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689766.1"
                     /translation="VADRSALDARVVSPGVHSGRANVPDSREDLGVASGRSLAFGARV
                     LGSGERILAARGLVVARSERFPAPGELVRVSSERFPTPGVCVPGSSERFRASGEFVRS
                     ASERFLAPDERVPGSSERLLASGEFVRSASERFLARSDDRLVQSVDVLAFSEDADAFR
                     ERLRAFSGDVLAVTGAAGIRRSAGRVHDGSGLVPRETRQPRIDGRLL"
     gene            704..1807
                     /locus_tag="VF453_18825"
     CDS             704..1807
                     /locus_tag="VF453_18825"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7689767.1"
                     /translation="MTEPSVPFAIDPDITRAHTLPSSFYLDPATYALARERVFARSWQ
                     WLGDTTDVTEPGSLSPREMLPGCLDEPLLLARDLGGELRCLSNVCTHRGNLLVEAPCR
                     GQQQIRCGYHSRRFDLAGRMTFMPAFETACDFPSAADHLPQVAFGTLGTQAFAAIAPV
                     APLDAFLGDLRARLGWLPLDEFRHDPSRDRAFDVAAHWALYVENYLEGLHIPFIHPAL
                     NQVLSFDGYAYELQRYANLQFARARDGEAAFELPASSPDHGERVAAYYYWIFPNLMLN
                     FYPWGLSVNVVEPRGIDRTRVLFRSYVWDAAKLGDGAGADLDQVEREDEAVVQQVQRG
                     VRSRLYDRGRYSPTKEQGVHQFHRLLQEFLAGE"
     gene            complement(1824..>2142)
                     /locus_tag="VF453_18830"
     CDS             complement(1824..>2142)
                     /locus_tag="VF453_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689768.1"
                     /translation="DLPPAVTSPTGIGGSTPLPGGYRPLPGGRDERIARDLDALEKES
                     QRLQAFNKRERCEHAKQQLEVAKAEMPIFTRDTNGERHYIEDRDRAANTAARRQEVQA
                     ACN"
BASE COUNT          263 a          783 c          749 g          347 t
ORIGIN      
        1 cgtcgctgat cgttccgccc tcgacgcccg cgtcgtctcg cccggcgtcc actcgggacg
       61 agccaatgtt cccgattcgc gcgaggacct tggcgtcgcg agcgggcggt cgctcgcgtt
      121 cggcgcgcgt gtcctcggtt ccggtgagcg aatcctcgct gcgcgcggcc tcgttgtcgc
      181 ccgaagcgag cgatttcccg ctcccggaga gctcgtccgc gtatcgagcg agcgattccc
      241 cactccgggc gtgtgcgtcc ccggttcaag cgagcgattc cgcgcttcgg gcgagttcgt
      301 ccgcagtgcg agcgagcgat tcctcgctcc ggacgagcgc gtccccggtt caagcgagcg
      361 attgctcgct tccggcgagt tcgtccgcag tgcgagcgag cgattcctcg ccaggagcga
      421 cgaccgcctc gtccaatccg tcgacgtcct cgctttttcc gaggacgccg acgcttttcg
      481 cgagcgtctc cgcgcgtttt ccggggatgt cctcgccgtg accggggcgg cgggcatccg
      541 gcgatccgcg ggccgcgtgc acgatggcag tggcctcgtc ccgagggaaa cacggcagcc
      601 ccgcatcgac ggccggctgc tataaccccg cgcagccctc cctccgggag acggcgacgg
      661 gcccgccgcg gcccgtcatg ccgccggcca acgaggatcc gagatgaccg agccctccgt
      721 ccccttcgcc atcgaccccg acatcacccg cgcgcacacg ctgccctcgt ccttctatct
      781 cgaccccgcg acgtacgcgc ttgccaggga acgcgtcttc gcccgcagct ggcagtggct
      841 cggcgacacg acggacgtca ccgagcccgg ctcgctgtcg ccgcgcgaga tgctgccggg
      901 atgcctggac gagccgctgc tgctcgcgcg cgacctgggc ggcgagctgc gctgcctgtc
      961 caacgtctgc acccatcgcg gcaacctgct ggtggaggcg ccgtgccgcg ggcagcagca
     1021 gatccgctgc gggtaccact cgcggcgctt cgacctcgcg gggcggatga ccttcatgcc
     1081 ggccttcgag acggcgtgcg atttcccgtc ggccgccgac cacctgccgc aggtcgcgtt
     1141 cggcacgctc gggacgcagg cgttcgcggc catcgccccc gtcgcgccgc tggacgcctt
     1201 cctcggcgac ctgcgcgccc gcctcggctg gctgccgctg gacgagttcc ggcacgaccc
     1261 gtcgcgcgac cgcgcgttcg acgtcgcggc gcactgggcg ctgtacgtcg agaactacct
     1321 cgaggggctg cacattccct tcatccaccc ggcgctgaac caggtgctgt ccttcgacgg
     1381 ctacgcctac gagctgcagc gctacgccaa cctgcagttc gcgcgggcgc gcgacggcga
     1441 ggcggcgttc gagctgccgg catcgtcgcc cgaccacggc gagcgggtcg cggcctacta
     1501 ctactggatc ttcccgaacc tgatgctcaa cttctacccg tgggggctgt cggtgaacgt
     1561 cgtcgagccg cgcgggatcg accgcacgcg ggtgctgttc cgcagctacg tgtgggatgc
     1621 ggcgaagctg ggcgacggcg cgggcgccga cctcgaccag gtcgagcggg aggacgaggc
     1681 ggtcgtgcag caggtgcagc gcggcgtgcg ctcgcggctg tacgaccgtg ggcgctattc
     1741 gccgaccaag gaacagggcg tgcaccagtt ccaccggctg ctgcaggagt tcctggccgg
     1801 ggagtgaacg gccgcgggcc gactcagttg caggccgcct gcacctcctg gcgccgcgcg
     1861 gccgtgttgg cggcgcgatc ccggtcctcg atgtagtggc gctcgccgtt ggtgtcgcgg
     1921 gtgaagatgg gcatctccgc cttggcgacc tccagctgct gcttggcgtg ctcgcagcgc
     1981 tcgcgcttgt tgaaggcctg caggcgctgg gactccttct cgagcgcgtc gagatcgcgc
     2041 gcgatgcgct cgtcgcgtcc gccgggcagc ggacgatagc cgcccggcag gggagtcgag
     2101 ccgccgatgc cggtcggcga cgtgacggcg ggcggcaggt cg
//
LOCUS       DASVLN010000795         4927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_95134, whole genome shotgun sequence.
ACCESSION   DASVLN010000795 DASVLN010000000
VERSION     DASVLN010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95134"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VF453_18835"
     CDS             complement(<1..240)
                     /locus_tag="VF453_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689769.1"
                     /translation="MALPPLVAAGARETRSTCPYCGTGCGVVIRSEGARIVDVRGDPE
                     HPANFGRLCTKGSTLHLTAAPHVERQARLLAPMRRT"
     gene            complement(255..659)
                     /gene="nirD"
                     /locus_tag="VF453_18840"
     CDS             complement(255..659)
                     /gene="nirD"
                     /locus_tag="VF453_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEX7689770.1"
                     /translation="MSDWKTICRLDDIPVQGARRVRRAHGGEVAVFRTADDRVFALLD
                     RCPHKGGPLSQGIVFGEHVACPLHNWAIGLADGTARLPDVGCTPTFRVRVEDGAVALL
                     ADELAHHATGEQAHASEDLCDARRVIAIAAAR"
     gene            complement(672..3116)
                     /gene="nirB"
                     /locus_tag="VF453_18845"
     CDS             complement(672..3116)
                     /gene="nirB"
                     /locus_tag="VF453_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEX7689771.1"
                     /translation="MKKMKLVMIGNGMAGVRTLEELLRIAPGLYDITVFGAEPHPNYN
                     RILLSPVLAGEQTVAEIVLNPLAWYADNGITLHLGKAVTEIDRRRRVVRAADGTEAAY
                     DRLLIATGSTPFILPVPGKDLAGVIGYRDIADTEAMIDAATRYRHAVVIGGGLLGLEA
                     ANGLMLRGMQVTVVHIAPWLMERQLDDVAGGLLRKSLVERGLDFRIGAHTEALLGDGA
                     GREERVRAVRFKDGTEVPAELVVMAAGIRPNTALAERAGLHVNRGIAVSDTLQTVTDA
                     RVYAVGECAAHRGVAYGLVAPLFEQAKVCATHLAEFGIGRYLGSQVSTKLKVTGIDLF
                     SAGDFMGGEGCDEIVLSDPQGGVYKKLVLKDDKLVGACLYGDTVDGSWYFKLLREGRP
                     VADIRDRLMFGESSLGDAGHQGQNKAAAMADADEVCGCNGVSKGAICKAIKEQGLFTL
                     DDVRKHTKASASCGSCTGLVEQLIMATAGGDYSAAPKKKAMCGCTEHSHGDVREAIRK
                     LHLTTIADTQRALSWRTPDGCPSCRPALNYYLVSTWPKEAKDDPQSRFINERSHANIQ
                     KDGTYSVIPRMWGGETSAAELRRIADVVDKYRIPTVKVTGGQRIDLLGVKKEDLQGVW
                     SDLGMPSGHAYAKALRTVKTCVGSEWCRFGTQDSTQMGKDLERGLWRMVAPHKVKLAV
                     SGCPRNCAESGIKDVGVIGVDSGWEIYVGGNGGIKTEAGHFFCKVRTAEEVMETAGAF
                     LQLYREEGWYLERTVHWIARVGLDGIKQRVLDDAANRQALWARLQSALDGEPDPWFEH
                     EKAAVDTRQFIPILAV"
     gene            complement(3154..4377)
                     /locus_tag="VF453_18850"
     CDS             complement(3154..4377)
                     /locus_tag="VF453_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7689772.1"
                     /translation="MTRFSAFLRTGHAPTLLAAFLYFGFSCCTWVLNGAMAPFISETF
                     HLGPAQKGLMLSVPIVAGALFRFPLGVLSQYIGRKRATLVEMSLISLAMLLGFFFVQS
                     FSALLAMGVLLGVAGASFGVAMSLGSGSFPAKHKGLAMGIVGAGNVGTAVAVLIAPPL
                     AQWLGWQTVYAVAACAMAIPMAAVIFLAREPQDVDPHAGLRAQLACLVERDGWAFSLI
                     YAVTFGGFIGFVSFLPSYYHDQFGVGKVQAGQLTTIVAFVGAATRVAGGWLSDRFGGV
                     NALTAVLATVAVGLAACGLATNSLALTTVLLVVCFAALGAGNGAVYQLVPLRWPLTTA
                     VAGSMIGELGALGGGLVPNAMGLARQFGGSFAWGFAFFALFALSTLVLLRALQGRWTT
                     SWVGAGGRALSAARS"
     gene            4670..>4927
                     /locus_tag="VF453_18855"
     CDS             4670..>4927
                     /locus_tag="VF453_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689773.1"
                     /translation="MPSALILDLAGPAFDDWRAALAGAGLHRGATSAGHELVQRTIAE
                     NGVDELVCLAEDAGAALERALAPWGGAPPCPVTAVLATPPDE"
BASE COUNT          687 a         1792 c         1772 g          676 t
ORIGIN      
        1 cgtgcgccgc atcggcgcga gcaggcgcgc ctggcgctcg acgtgcggcg ccgcggtcag
       61 gtgcagcgtg ctgcccttgg tgcacaggcg gccgaagttg gcgggatgct cgggatcgcc
      121 gcgcacgtcg acgatgcgcg cgccctcgct gcgaatcacg acgccgcaac ccgtgccgca
      181 gtacgggcag gtcgagcgcg tctcgcgcgc gccggcggcg accaggggcg gcagcgccat
      241 cgttcgcgtt cgcgtcagcg ggccgcggcg atggcgatca cgcgccgcgc gtcgcagagg
      301 tcttccgacg cgtgcgcctg ctcgccggtc gcgtgatggg cgagctcgtc ggccagcagc
      361 gcgaccgcgc cgtcctcgac ccgcacgcgg aaggtcggcg tgcagccgac gtccggcagc
      421 cgcgccgtgc cgtcggccag cccgatcgcc cagttgtgca gcggacacgc cacgtgctcg
      481 ccgaacacga tgccctggct cagcggcccg cccttgtgcg ggcagcgatc gagcagcgcg
      541 aacacgcggt cgtcggcggt gcggaagacc gcgacctcgc cgccgtgcgc gcgccgcacg
      601 cggcgggcgc cctgcacggg gatgtcgtcg aggcggcaga tcgttttcca gtcgctcatg
      661 gtcatgtctc ctcacaccgc caggatcggg atgaactgcc gcgtgtcgac cgcggccttc
      721 tcgtgctcga accacggatc gggctcgccg tcgagcgcgg actgcaggcg cgcccacagc
      781 gcctggcggt tggccgcgtc gtcgagcacg cgctgcttga tgccgtccag gccgacgcgc
      841 gcgatccagt gcacggtgcg ctcgaggtac cagccctcct cgcgatagag ctgcaggaac
      901 gcgcccgcgg tctccatcac ctcctcggcg gtgcgcacct tgcagaagaa gtgcccggcc
      961 tcggtcttga tgccgccgtt gccgccgacg tagatctccc agccggagtc cacgccgatc
     1021 acgcccacgt ccttgatgcc cgactcggcg cagttgcgcg ggcagcccga gaccgcgagc
     1081 ttgaccttgt gcggcgcgac catgcgccac agcccgcgct cgaggtcctt gcccatctgc
     1141 gtcgagtcct gcgtgccgaa gcggcaccac tcgctgccga cgcaggtctt gacggtgcgc
     1201 agcgccttcg cgtacgcgtg ccccgagggc atgccgaggt cgctccagac gccctgcagg
     1261 tcctccttct tcacgcccag caggtcgatg cgctggccgc ccgtcacctt gaccgtcggg
     1321 atgcggtact tgtcgaccac gtcggcgatg cggcgcaact ccgccgcgct cgtctcgccg
     1381 ccccacatgc gcgggatcac cgagtaggtg ccgtccttct ggatgttggc gtgcgagcgc
     1441 tcgttgatga agcggctctg cgggtcgtcc ttcgcctcct tgggccaggt gctcacgagg
     1501 tagtagttca gcgccgggcg gcaggacgga cagccgtcgg gcgtgcgcca cgacagcgcg
     1561 cgctgggtgt cggcgatcgt cgtcaggtgc agcttgcgga tcgcctcgcg cacgtcgccg
     1621 tgcgagtgct cggtgcagcc gcacatcgcc ttcttcttcg gcgcggcgga gtagtcgccg
     1681 cccgcggtcg ccatgatcag ctgctcgacg aggcccgtgc acgagccgca cgacgcgctc
     1741 gccttcgtgt gcttgcgcac gtcgtcgagc gtgaacaggc cctgctcctt gatcgccttg
     1801 cagatcgcgc ccttgctgac gccgttgcag ccgcagacct cgtcggcgtc ggccatcgcg
     1861 gcggccttgt tctggccctg gtggccggcg tcgccgaggc tggactcgcc gaacatcagg
     1921 cggtcgcgga tgtcggccac cggccggccc tcgcgcagca gcttgaagta ccagctgccg
     1981 tcgaccgtgt cgccgtacag gcaggcgccg accagcttgt cgtccttgag caccagcttc
     2041 ttgtagacgc cgccctgcgg gtcggacagc acgatctcgt cgcagccttc gccgcccatg
     2101 aagtcgccgg ccgagaacag gtcgatgccg gtgaccttca gcttggtcga gacctggctg
     2161 ccgaggtagc ggccgatgcc gaactcggcc aggtgcgtcg cgcagacctt ggcctgctcg
     2221 aacagcggcg cgaccagccc gtaggcgacg ccgcggtgcg ccgcgcactc gcccacggcg
     2281 tagacgcgcg cgtcggtgac ggtctgcagc gtgtcgctga cggcgatgcc gcggttcacg
     2341 tgcaggccgg cgcgctcggc cagcgccgtg ttggggcgga tgcccgcggc catcacgacg
     2401 agctcggcgg gcacctccgt gccgtccttg aagcgcaccg ctcgaacccg ttcctcgcgg
     2461 ccggcaccgt cgcccagcag cgcctcggtg tgcgcgccga tgcggaagtc caggccgcgc
     2521 tcgacgagcg acttgcgcag caggccgccc gcgacgtcgt cgagctggcg ctccatcagc
     2581 cagggcgcga tgtgcaccac ggtgacctgc atgccgcgca gcatcaggcc gttggccgcc
     2641 tccagcccga gcaggccgcc gccgatcacg accgcatgcc ggtacctggt cgcggcgtcg
     2701 atcatggcct cggtatcggc gatgtcgcgg tagccgatca cgcccgcgag atccttgccc
     2761 ggcaccggca ggatgaaggg cgtcgagccg gtggcgatca gcagccggtc gtaggcggcc
     2821 tcggtgccgt cggcggcgcg cacgactcgg cggcgccggt cgatctccgt caccgccttg
     2881 cccaggtgca gcgtgatgcc gttgtccgcg taccaggcga gcgggttgag cacgatctcc
     2941 gcgaccgtct gctcgcccgc gagcaccggc gacagcagga tgcggttgta gttcggatgc
     3001 ggctcggcgc cgaagacggt gatgtcgtac aggccgggcg cgatcctgag cagctcctcg
     3061 agcgtgcgca cgccggccat gccgttgccg atcatcacga gcttcatctt cttcatggcg
     3121 ggatctcttc cgaggtggat gcggggatcg ggttcaggag cgcgccgccg acagcgcgcg
     3181 gccgcccgcg ccgacccagc tggtcgtcca gcggccctgc agcgcgcgca gcagcacgag
     3241 cgtcgacagc gcgaacagcg cgaagaacgc gaagccccac gcgaagctgc cgccgaactg
     3301 gcgcgccagc cccatggcgt tgggcaccag gcccccgccc agcgcgccga gctcgccgat
     3361 catcgagccg gcgacggcgg tcgtgagcgg ccagcgcagc ggcaccagct ggtagaccgc
     3421 gccgttgcct gcgcccagcg ccgcgaagca cacgaccagc agcacggtcg tcagcgcgag
     3481 cgagttcgtg gccaggccgc acgcggcgag gcccaccgcg acggtcgcca gcacggccgt
     3541 cagggcgttg acgccgccga agcgatccga cagccagccg cccgccacgc gcgtcgccgc
     3601 gccgacgaag gcgacgatcg tcgtgagctg gccggcctgc accttgccga cgccgaactg
     3661 gtcgtggtaa tacgaaggca ggaagctcac gaagccgatg aagccgccga aggtcaccgc
     3721 gtagatcagg ctgaaggccc agccgtcgcg ttcgacgagg caggcgagct gcgcgcgcag
     3781 gccggcgtgc gggtcgacgt cctgcggctc gcgggcgagg aagatcaccg cggccatcgg
     3841 gatcgccatc gcgcacgcgg ccacggcgta gaccgtctgc cagccgagcc actgggcgag
     3901 cggtggcgcg atcagcacgg ccacggcggt gcccacgttg cccgcgccga cgatgcccat
     3961 cgcgaggccc ttgtgcttcg cgggaaacga gcccgagccc agcgacatcg cgacgccgaa
     4021 gctcgcgccc gcgacgccca gcagcacgcc catcgccagc agcgcggaaa agctctgcac
     4081 gaagaagaag cccagcagca tcgccaacga gatcaggctc atctcgacca gcgtcgcgcg
     4141 cttgcggccg atgtattgcg acagcacgcc cagcgggaag cggaacagcg cgcccgcgac
     4201 gatgggcacc gacagcatca ggcccttctg cgccgggccc aggtggaagg tctcgctgat
     4261 gaacggcgcc atcgccccgt tcagcaccca ggtgcagcac gagaagccga agtacaggaa
     4321 ggcggcgagc agcgtcggcg cgtggccggt gcggaggaag gcgctgaagc gggtcatggg
     4381 cggttccgtg gcgtggggtg gcagggacga tgcggcggcg cgcgcagggc gcgcacgggc
     4441 atcgggcgag gggaggcgtg ggccggcggc gcgtcggacg cggcgcgtgc ggcgggagga
     4501 acccggcgat cgcggatcga ggtcggcacc gggggtgccg ttcaccgggg cggtgcgtcg
     4561 ttgcaccgcg tgccgacctt cacgcaagct ccgtgccgga tcggcggcat ggtccgtgca
     4621 agcgtccccg gcgacggcgc cggcgcgcgc cgccctcctc gtgacgacga tgccctccgc
     4681 cctgatcctc gacctcgccg ggcccgcctt cgacgactgg cgcgccgcgc tggccggcgc
     4741 cggcctgcac cgcggcgcga catcggcggg ccacgaattg gtgcagcgca ccatcgcgga
     4801 gaacggcgtg gacgagctcg tctgccttgc cgaggacgcc ggcgcggcac tcgagcgggc
     4861 gctggcgccc tggggcggcg cgccgccctg cccggtgacc gccgtgctgg cgacgccgcc
     4921 ggacgag
//
LOCUS       DASVLN010000796         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_526627, whole genome shotgun sequence.
ACCESSION   DASVLN010000796 DASVLN010000000
VERSION     DASVLN010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526627"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /gene="fliP"
                     /locus_tag="VF453_18860"
     CDS             <1..695
                     /gene="fliP"
                     /locus_tag="VF453_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548311.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX7689774.1"
                     /translation="AAATPAAPSLPLLVGAGGGGTSYSVPIQTLLFFTALSFLPAVLL
                     MMTGFTRIVIVLSLFRQAIGTQAAPPNQVIVGMSLFLTFFVMSPTIDKVYTDAWQPYQ
                     ANQISFTEALDRGQVPVRSFMLKQTRQSDVELFAKLAKLPASVKGEQVPFKVLVPAFV
                     ASELKSAFQIGFLIFIPFLIIDMIVASVLMSLGMMMLSPVLVALPFKLMLFVLADGWN
                     LLLGSLAASFVT"
     gene            778..1047
                     /gene="fliQ"
                     /locus_tag="VF453_18865"
     CDS             778..1047
                     /gene="fliQ"
                     /locus_tag="VF453_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX7689775.1"
                     /translation="MDAAQVFTVGQQALFTMLTVAAPVLGVVLVVGILVSVFQAATQI
                     NEATLTFVPKLLAAVAVMGLAGPWMLTSLVEYLRSVLLSLPQATS"
     gene            1086..1847
                     /gene="fliR"
                     /locus_tag="VF453_18870"
     CDS             1086..1847
                     /gene="fliR"
                     /locus_tag="VF453_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX7689776.1"
                     /translation="MLTLNEAQIMAWLAPVLWPFLRVLALFTAMPVLGQRNVPARVRI
                     ALSFFIALGVAGTAPAGPSVALDSPAAVMLVVQQVLIGVALGFAVRVIFTAIEFAGEI
                     VGLQMGLNFAGFFDPATSQQATATSSFFSTMVGFLFIAMNGHLLVIEAVVQSLTAFPV
                     SAEPFAFLHAVRPETWGAEIFRLGLWIALPLIAMLLFVNMMMGIIARVAPQISIFSVG
                     FPVTLAVGLVGIMFTLPLLQGPFTAALEQMLMLFR"
BASE COUNT          252 a          751 c          675 g          388 t
ORIGIN      
        1 aggccgccgc gacgcccgcc gcgccgtcgc ttccgctgct cgtcggcgcg ggcggcgggg
       61 gcaccagcta ctcggtgccg atccagacgc tgctgttttt caccgcgctg tccttcctgc
      121 ccgcggtgct gctgatgatg accggcttca cgcgcatcgt gatcgtgctg tcgctgttcc
      181 gccaggcgat cggcacccag gccgcgccgc cgaaccaggt catcgtcggg atgtcgctgt
      241 tcctcacgtt cttcgtgatg tcgccgacca tcgacaaggt ctacaccgac gcgtggcagc
      301 cctaccaggc caaccagatc agcttcaccg aggcgctcga ccgcggccag gtgccggtgc
      361 gctcgttcat gctcaagcag acgcggcagt cggacgtgga gctgttcgcc aagctcgcga
      421 agctgcccgc gtcggtcaag ggcgagcagg tgccgttcaa ggtgctggtg ccggccttcg
      481 tcgcgagcga gctcaagagc gcgttccaga tcggcttcct gatcttcatc ccgttcctca
      541 tcatcgacat gatcgtcgcc agcgtgctga tgtcgctggg catgatgatg ctgtcgccgg
      601 tgctggtggc gctgccgttc aagctcatgc tgttcgtcct ggccgacggc tggaacctgc
      661 tgctgggatc actcgcggcg agcttcgtga cctgaaacac cgcgcttttc gaggcctcgc
      721 gatcgcgggg tcccgataac gtgtggccat cgtcacccgc ccaccgttcc cgcatccatg
      781 gacgccgccc aagtcttcac cgtcggccag caggccctgt tcaccatgct caccgtcgcc
      841 gcgcccgtgc tgggcgtcgt gctggtcgtg ggcatcctcg tcagcgtgtt ccaggcggcg
      901 acgcagatca acgaggcgac gctgaccttc gtgcccaagc tgctggccgc cgtcgccgtg
      961 atgggcctgg cggggccgtg gatgctgacc tcgctggtcg agtacctccg cagcgtcctg
     1021 ctgtccctgc cgcaagcgac cagttgaagg aagcggcggc ccgccgcgcc gcttaacatg
     1081 ccgccatgct gacgctgaac gaagcccaga tcatggcctg gctcgcgccg gtgctgtggc
     1141 cgttcctgcg cgtgctggcg ctgttcacgg cgatgcccgt gctcggccag cgcaacgtgc
     1201 cggcgcgcgt ccgcatcgcg ctgtcgttct tcatcgcgct gggcgtcgcc ggcaccgcgc
     1261 ccgcgggacc gagcgtcgcg ctggactcgc ccgccgccgt catgctcgtc gtccagcagg
     1321 tgctgatcgg cgtcgccctc ggcttcgcgg tgcgcgtcat cttcacggcc atcgagttcg
     1381 ccggcgagat cgtgggcctg cagatgggcc tgaacttcgc gggcttcttc gatcccgcga
     1441 cctcgcagca ggcgaccgcg accagcagct tcttctcgac gatggtgggc ttcctgttca
     1501 tcgcgatgaa cggccacctg ctcgtgatcg aggcggtcgt gcagagcctc accgcgttcc
     1561 cggtctcggc cgagcccttc gccttcctgc acgcggtgcg gcccgagacc tggggcgccg
     1621 agatcttccg cctcgggctg tggatcgcgc tgccgctgat cgcgatgctg ctgttcgtca
     1681 acatgatgat gggcatcatc gcgcgcgtgg cgccgcagat cagcatcttc tcggtcggct
     1741 tcccggtcac gctcgccgtg ggcctcgtgg gcatcatgtt cacgctgccg ctgctgcagg
     1801 ggccgttcac ggcggcgctc gagcagatgc tcatgctgtt ccgctgagta cactgccggg
     1861 ctccgcagcc cgtctgccgc gccgcaggcc cgaagcgatg ccccacgccg gatcgcgccc
     1921 caccgccctc atcgacttcc gctcgcccga cgacggcggc gcgccgctgc gactcgcgtt
     1981 cggcgcgccg cgcgagcgcc tgcgcgcact gacgctggac gaggtcgtgc cggtgctcgc
     2041 gcgcgtcgac gccctcgcgt gcgccg
//
LOCUS       DASVLN010000797         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_152017, whole genome shotgun sequence.
ACCESSION   DASVLN010000797 DASVLN010000000
VERSION     DASVLN010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152017"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1160)
                     /locus_tag="VF453_18875"
     CDS             complement(66..1160)
                     /locus_tag="VF453_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7689777.1"
                     /translation="MTVRVPVRAALRALLPALVLAGLAAQASANTLTQNVSWTIDRSG
                     TSTKYRLVAYGDSIYAGYNTSITSAAKYAAPTVDAEYLSALWNADIESVRRAKSGAVA
                     SDVYANKIVAEKSYMQASSTRVVTFEMCGNDGLQARTAFKSQSGTCDYSGMDNAVSTC
                     KQYVAQAMDFINANAYAGVKVKVISNLHYPGYAADNTQSSCKDATTKATVNLQNTFLP
                     RLAKMNYWMCEYAREKGFTCADNFAGFMGADYDSNGDGIVDAAGLAWVPGESEASYVT
                     RITTTLRSTIRDANTHFVSASSSYDYIQSDDTHPTYTGGTVTAGLFGGTTGSGAPRYT
                     SFSNGKNPIWNQYGHERMGWTVSTSNTSAP"
     gene            complement(1415..>1665)
                     /locus_tag="VF453_18880"
     CDS             complement(1415..>1665)
                     /locus_tag="VF453_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HEX7689778.1"
                     /translation="ARLVQRLRLAGDAREAVPLEVLAAGPDFPANALGRKVRAGRHVG
                     EVVRLSPDGAQLSMVNERSGATQLFDVAFWRAKGRPAR"
BASE COUNT          219 a          562 c          607 g          277 t
ORIGIN      
        1 cccccagccg cgccggccgg cgccgtcctt gccgccgcgt ggctcgccca tcgttcaggc
       61 cccggtcacg gcgcggacgt gttggacgtc gacaccgtcc accccatccg ctcgtggccg
      121 tactggttcc agatcgggtt cttgccgttc gagaacgagg tgtagcgcgg cgcgccggag
      181 cccgtggtgc cgccgaacag gcccgcggtc accgtgccgc ccgtgtacgt cgggtgggta
      241 tcgtccgact ggatgtagtc gtagctcgag ctcgccgaga cgaagtgcgt gttggcgtcg
      301 cggatggtcg agcgcagcgt cgtcgtgatg cgcgtcacgt agctcgcctc gctctcgccc
      361 ggcacccagg ccaggcccgc ggcgtcgacg atgccgtcgc cgttgctgtc gtagtcggcg
      421 cccatgaagc cggcgaagtt gtcggcgcag gtgaagccct tctcgcgcgc gtattcgcac
      481 atccagtagt tcatcttcgc caggcgcggc aggaaggtgt tctgcaggtt gacggtggcc
      541 ttggtcgtgg cgtccttgca ggagctctgc gtgttgtcgg cggcgtagcc cgggtagtgc
      601 aggttggaga tcaccttgac cttcacgccc gcgtaggcgt tggcgttgat gaagtccatc
      661 gcctgcgcga cgtactgctt gcaggtgctg accgcgttgt ccatgccgga gtagtcgcac
      721 gtgccgctct ggctcttgaa cgcggtgcgc gcctgcaggc cgtcgttgcc gcacatctcg
      781 aaggtcacca cgcgcgtcga gctggcctgc atgtaggact tctcggccac gatcttgttg
      841 gcgtagacgt cggacgcgac ggcgcccgac ttcgcgcgcc gcacgctctc gatgtcggcg
      901 ttccacagcg cggacaggta ttccgcgtcg acggtcggcg cggcgtactt ggccgcgctg
      961 gtgatcgacg tgttgtagcc ggcgtagatc gagtcgccat aggccacgag gcggtacttg
     1021 gtcgaggtgc cggaacggtc gatcgtccac gacacgttct gggtcagcgt gttggcggag
     1081 gcctgcgcgg ccaggccggc gaggacgagg gcgggcagca gcgcgcgcag ggcggcgcgc
     1141 acgggaacgc ggacggtcat cggtgtctcc tgggcttgtc gtgcatgcga ggggaacgca
     1201 gcgtccgctg ccgctgcagg cggccgccga cgtgccgcga gactagtcac ccgacccgcg
     1261 cgggagatcc ggacgaaccc tcgccgcgcc gcccggccgg agcgcgcggc caagctcttg
     1321 gctcgccggg tcagcgcatg tgcaaatagc gcgcagtctg acgcccttga tacgcccgtg
     1381 ggtatcgggg cgacggcgcg gtgcaagcac ccgctcagcg cgccggacga cccttggcgc
     1441 gccagaacgc gacgtcgaag agctgggtcg cgccgctgcg ctcgttgacc atggacagct
     1501 gcgctccgtc gggactgagc cgcacgacct cgccgacatg gcggcccgcg cgcaccttgc
     1561 ggccgagcgc gttggcggga aagtccggcc cggcggcgag cacctccagc ggcacggcct
     1621 cgcgcgcgtc gccggccagg cgcaggcgct gcacgaggcg cgcga
//
LOCUS       DASVLN010000798         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_202248, whole genome shotgun sequence.
ACCESSION   DASVLN010000798 DASVLN010000000
VERSION     DASVLN010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202248"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /gene="fmt"
                     /locus_tag="VF453_18885"
     CDS             complement(<1..844)
                     /gene="fmt"
                     /locus_tag="VF453_18885"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX7689779.1"
                     /translation="MRVAFAGTPEFAAAALQAIHAAGYPVPLVLTQPDRPAGRGMKLT
                     PSPVKQLALDIGAAVAQPRSLRLDGRFADEAESGRRALAHAAPDVMVVAAYGLILPQW
                     VLDLPRLGCLNIHGSLLPRWRGAAPIHRAIEAGDAETGITIMQMDAGLDTGAMLSMER
                     EPIHADDTTATLHDRLAALGARMVVQALDDAARGALAPVAQPDEGVTYAHKIAKAEAA
                     IDWTLPAPVVERRVRAFDPFPGAAFTLGGEQVKVWRAAVEPLAVTAAPGTVVAADDRR
                     LVVAC"
     gene            complement(874..1389)
                     /gene="def"
                     /locus_tag="VF453_18890"
     CDS             complement(874..1389)
                     /gene="def"
                     /locus_tag="VF453_18890"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7689780.1"
                     /translation="MARLPILRYPDPRLYTVAKPVAVVDDRIRRLADDMLETMYASDG
                     VGLAATQVDVHERLIVMDTSETHDQPRVLINPEVVTGSDDWILGEEGCLSVPQIYEKV
                     ERHARVRVRALDRDGQPYEFDAEGLAAVCVQHEMDHLMGKVFVDYLSPLKRDRIKTKM
                     LKRTREEQTRR"
     gene            1766..2878
                     /locus_tag="VF453_18895"
     CDS             1766..2878
                     /locus_tag="VF453_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEX7689781.1"
                     /translation="MLAVAPSLTFAATYPVTQGQRQTAREVAARGVPLSELAANAPDT
                     YTVKRGDTLWAISRMFLKSPWRWPELWGMNLQQIHNPNLIYPGQLLVLEKIDGMARLK
                     MGNVIGGNDTVKLSPTIRSESSETGAIAAIPMNAISPFLNDALVFDSNELAKAPRVVA
                     TQEGHVALARGDRAYVLGDLGDTRSWQLFREPKPLVDPDTHELLGYEARYVGSADRVL
                     DGESRPGAGQEKVAVPSTFVLTSTREDAEVGDRLAPATVRDFAPFVPHAPASPIGGTV
                     VSLYGDQLSSGTNQVIAINRGARDGVERGHVLTLWHQGALTHDKSVEHGSLIKLPDEK
                     VGTMMVFRVFDRVSYALIMESTEPVLPGDRFSSPAP"
     gene            3391..>3519
                     /locus_tag="VF453_18900"
     CDS             3391..>3519
                     /locus_tag="VF453_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEX7689782.1"
                     /translation="MSRDELAAWLRLVETPTLGRAGARRLLAAFGSPERVLGADERA"
BASE COUNT          489 a         1297 c         1226 g          507 t
ORIGIN      
        1 cgcaggcgac gacgaggcgc cggtcgtcgg ccgcgaccac cgtgccgggg gcggccgtga
       61 cggccagcgg ctcgaccgcg gcgcgccaga ccttcacctg ctcgccgccg agcgtaaagg
      121 ccgcgcccgg gaagggatcg aacgcgcgca ctcggcgctc gacgacgggg gccggcagcg
      181 tccagtcgat cgccgcctcg gccttggcga tcttgtgggc gtacgtgacg ccttcgtccg
      241 gctgcgcgac cggggcgagc gcgccgcgcg cggcatcgtc cagcgcctgc acgaccatgc
      301 gcgcgccgag cgcggccagg cggtcgtgca gcgtggcggt ggtgtcgtcg gcatggatgg
      361 gctcgcgctc catcgacagc atggcgccgg tgtccagccc ggcgtccatc tgcatgatcg
      421 tgatgccggt ctcggcgtcg cccgcctcga tcgcccggtg gatgggcgcc gcgccgcgcc
      481 agcgcggcag caacgagccg tggatgttca ggcagcccag gcgcggcagg tcgagcaccc
      541 actgcggcag gatcagcccg taggcggcca ccaccatcac gtccggcgcg gcgtgcgcga
      601 gcgcccggcg gcccgactcg gcctcgtcgg cgaacctgcc atccagccgc aggctgcgcg
      661 gctgcgcgac cgccgcgccg atgtccaggg cgagctgctt gaccggcgac ggcgtcagct
      721 tcatgccgcg gccggcgggc cggtcgggct gcgtcagcac cagcggcacc ggatagccgg
      781 ccgcatggat cgcctgcagc gcggcggcgg cgaactccgg cgtgcccgcg aaggcgacgc
      841 gcagggtgcg cgggatcgtc gcggcgccgg ccatcagcgg cgcgtctgct cttcgcgcgt
      901 gcgcttgagc atcttcgtct tgatgcggtc gcgcttgagc gggctcaggt agtcgacgaa
      961 gaccttgccc atcaggtgat ccatctcgtg ctggacgcag accgcggcga ggccctcggc
     1021 gtcgaactcg tagggctggc cgtcgcggtc gagcgcacgc acccgcacgc gcgcgtggcg
     1081 ctcgaccttc tcgtagatct ggggcaccga caggcagccc tcctcgccga ggatccagtc
     1141 gtccgagccg gtcacgacct ccggattgat cagcacgcgc ggctggtcgt gcgtctcgga
     1201 ggtgtccatg acgatgaggc gctcgtgcac gtcgacctgc gtcgcggcga ggccgacgcc
     1261 gtcggaggcg tacatggtct cgagcatgtc gtccgcgagg cggcggatgc ggtcgtcgac
     1321 gacggcgacg ggcttggcga cggtgtacag gcgcgggtcg gggtagcgga gaatcgggag
     1381 tcgggccatg aaagacggtt cctggaaatc tgccacgaaa accgccggaa atgccgccgg
     1441 cgcgcttgcg gcgccggtgc agaatgtgtc tgcgacgcga cgccggtact cagggtgatt
     1501 gcctacacac ttcgtaaccg agtgcgcgtt tcattgcgac ctcgacagtt cgcgtgcaga
     1561 atcggcggca ttttcgcggg tttcgagcga ctggatactc tccagccgga atcggcaatt
     1621 gttgcactgc acgcggtttc cgtgtcactt cggtcccaat cgccttcgca ggcccgggag
     1681 ttttctgcac atgtcccttc gttttcgact ttccggcggc tcgcggtccg cggcgaccct
     1741 gaacggcgtc gccctcgcgg ctgcgatgct ggccgtcgcg ccgtcgctga ctttcgcggc
     1801 gacgtatccc gtcacgcagg gccagcgcca gacggcgcgc gaagtcgcgg cccggggcgt
     1861 gccgctgtcc gagctggccg ccaacgcgcc ggacacgtat acggtcaagc gcggcgatac
     1921 gctgtgggcg atttcgcgga tgttcctcaa aagcccgtgg cgctggcccg agctgtgggg
     1981 aatgaatctg cagcagattc acaaccccaa cctgatctat ccggggcagc tgctcgtgct
     2041 cgagaaaatc gacggcatgg cgcggctgaa aatgggcaac gtcatcggcg gcaacgacac
     2101 ggtcaaactc tcgccgacca ttcgcagcga atcctcggag accggcgcca tcgccgcgat
     2161 cccgatgaac gcgatcagcc ccttcctcaa cgacgcgctc gtgttcgatt ccaacgagct
     2221 cgccaaggcg cctcgcgtcg tggcgacgca ggaaggccat gtcgcgctgg cgcgcggcga
     2281 ccgcgcctac gtgctgggcg acctcggcga cacgcgcagc tggcagctct tccgtgaacc
     2341 gaagccgctg gtcgacccgg acacgcacga gctcctcggc tacgaggcgc gctacgtcgg
     2401 ttcggccgac cgcgtgctgg acggcgagtc gcgtcccggc gccggccagg agaaggtggc
     2461 cgtgccctcg accttcgtgc tgaccagcac ccgcgaggac gccgaggtcg gcgaccgcct
     2521 cgcgccggcc acggtgcgcg acttcgcgcc cttcgttccg cacgccccgg cttcccccat
     2581 cggcggcacc gtcgtctcgc tctacggcga ccagctgtcc agcggcacca accaggtcat
     2641 cgccatcaac cggggcgcgc gcgacggcgt cgagcgcggc cacgtgctca cgctgtggca
     2701 ccagggcgcg ctcacgcacg acaagtcggt cgagcacggc agcctgatca agctgccgga
     2761 cgagaaggtc ggcacgatga tggtcttccg cgtcttcgac cgcgtgtcgt acgcgctcat
     2821 catggagagc acggagccgg tgctgcccgg cgaccgcttc agctcgcccg cgccctgagg
     2881 cgcccatcgc cggaccacgc gcggtccggc cctccgccgg gcgagccctc gcccggcccc
     2941 gcgacgcgca tggcgcgccg tctccgcgac ccggccgtgc cggcggctcg ccttcccgaa
     3001 acgacctccg cccggtgggc gcctcgcgcg cgggcgcagc cgcctcgtcg tcaagacaac
     3061 gccggccaca cgtcgtcgcc gacgcgatga ccgcgagcgc ttgcgaaagc cgcgggcctg
     3121 cccctcgatc gcgcggaggc ggcagtcggc cgcccatcga gagcaccacc tgacggacga
     3181 gccgcgaccg ccaagcgtgc cgcttgcccg catggcccga aaggcccgta aggcccctat
     3241 tcgggcgccc gtaggcgtgg cccgagcgcg cggcgctcgc gcctgccccg ccgtccaccg
     3301 tcgcggatgt cccgacgatc gaacccttcg cgccaccgtc cgtccaaccc ccggcattgc
     3361 cgccttctcg ccccaggccg tcccacgatg atctcccgcg acgaactcgc cgcctggctg
     3421 cggctggtcg agacaccgac gctcggccgc gccggcgcgc gccggctgct ggccgccttc
     3481 ggctcgcccg aacgcgtgct gggcgccgac gagcgcgcg
//
LOCUS       DASVLN010000799         4661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_40137, whole genome shotgun sequence.
ACCESSION   DASVLN010000799 DASVLN010000000
VERSION     DASVLN010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40137"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..949)
                     /locus_tag="VF453_18905"
     CDS             complement(26..949)
                     /locus_tag="VF453_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689783.1"
                     /translation="MSDLASPPTFFGYWAGRVPALAELHVRSLLHHHPHARYELWLDT
                     DLASTVEGPELRWLASDPRIVLRAFSLDALIERHVTRRTVRARDPLAGLRRAGRALHR
                     LVGPQWTRRQAWEHPDKGLSYRHGSALFAGFRADRVYRSRLARCLVPLEHYAGPCLYA
                     DLDVAFAADLLHHWGDKAWVYRWQARAHGCPSVMYLPNRSWSAALVRRGNELGSYEPW
                     VQFSDAECAALGIVVQPARLFDPLWDSTSLLYGEPERFLARRDHLALDLHALATERHL
                     AIRWHAPWDARADAESLYAGMLRACVDGTAE"
     gene            complement(961..1668)
                     /locus_tag="VF453_18910"
     CDS             complement(961..1668)
                     /locus_tag="VF453_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7689784.1"
                     /translation="MKWGTKYGPEYVNRLYAMVHRHLRGPFRFVCLTDSSAGVRREVE
                     CLPIPELGLVEGKGPDRAWKKLLTFDADLHGLRGTALFLDIDIVIVDDITPFFELPGE
                     FRIIHDWKRPWRVTGNSSVYRFQLGAHPEVLARLRAEFDTIRKRFRNEQAYLSAVMHE
                     QGKLQYWPDEWCPSFKYHSIPRFPLNFVRAPVIPEGARIVIFHGVVNPPDALVGRSNG
                     NWRSPRATPWIADHWKE"
     gene            1843..2415
                     /locus_tag="VF453_18915"
     CDS             1843..2415
                     /locus_tag="VF453_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7689785.1"
                     /translation="MSSLFLRNLAEHQALMTALPALDEAIAAAGRRLAAALAAGGKIM
                     FCGNGGSAADSQHLASELTGRFIEDRRPLAGLALSTDSSALTCIGNDYSFDDVFARQV
                     AGLGRAGDALVAISTSGNSRNVLRAVAAARQAGVFTLGLLGRDGGQLRAACDAAIVVP
                     SQVTARIQEAHILIGHTLCGAIEHELGLVE"
     gene            2474..3418
                     /gene="rfaE1"
                     /locus_tag="VF453_18920"
     CDS             2474..3418
                     /gene="rfaE1"
                     /locus_tag="VF453_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEX7689786.1"
                     /translation="MSAAPVLPPLAELERRRILVVGDVMLDRYWYGAVERISPEAPVP
                     VVRVEREEERLGGAANVALNVKTLGARATLLTVVGQDHAADGLRKLLGAHGIEAVLRT
                     DPQLSTIVKLRVIGRAQQLIRVDFENEPDHEVLGQMVDAFAEQLPAHDVVLFSDYGKG
                     GLAHIPRMIELARAAGKPVLVDPKGSDYRRYAGASVVTPNRAELAQVVGTWRDERELA
                     ARVAALRADIGVEAVLLTRSEEGMTLFADGQTVHEPARAREVFDVTGAGDTVIATLAS
                     LLAAGLPLDASMRLANKAGGIVVGKFGTATVSYEELAA"
     gene            3463..3921
                     /locus_tag="VF453_18925"
     CDS             3463..3921
                     /locus_tag="VF453_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEX7689787.1"
                     /translation="MDDAALADRLAALPRPLVFTNGVFDVLHRGHVAYLHAARRLGAS
                     LVVAVNSDASARGLGKGPDRPLNRDVDRALVLAGLAAVDAVTFFDEPTPCALLARVRP
                     QLYVKGGDYDMETLEETRLVRSWGGEALAIAFVEGYSTTALVRRIRAGAA"
     gene            3921..4472
                     /gene="gmhB"
                     /locus_tag="VF453_18930"
     CDS             3921..4472
                     /gene="gmhB"
                     /locus_tag="VF453_18930"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:TIGR00213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEX7689788.1"
                     /translation="MRRAAFLDRDGVVNVDRGYLHRWEDFEFLPGAVAAMRRLQDAGF
                     ALVVVTNQSGIARGYYAEDDFVRLTDAMWAALVGEGVSLAAVEWCPHHPRGSVARYAI
                     ACDCRKPEPGMILRAAKALDIDLAASVLFGDKPSDIEAARRAGVGRAILVAPNAGAGS
                     PDDDEAAIPEFASLAEAVDAYLG"
BASE COUNT          611 a         1708 c         1721 g          621 t
ORIGIN      
        1 cgcccggcgc ccgcgggggc gaccttcatt ccgccgtgcc gtcgacgcag gcccgcagca
       61 tccccgcgta cagcgactcc gcgtccgccc gcgcgtccca cggcgcgtgc cagcggatcg
      121 ccaggtggcg ctccgtggcc agcgcgtgca ggtcgagcgc caggtggtcg cggcgcgcca
      181 ggaagcgctc gggttcgccg tacagcagcg aggtggagtc ccacagcggg tcgaacagcc
      241 gcgccggctg gacgacgatg cccagcgcgg cgcattcggc gtccgagaac tgcacccagg
      301 gctcgtagct gccgagttcg ttgcctcgcc gcaccagcgc cgcgctccag ctgcggttgg
      361 gcaggtacat caccgacggg cagccatggg cgcgcgcctg ccagcggtag acccaggcct
      421 tgtcgcccca gtggtgcagc aggtcggcgg cgaaggcgac gtcgaggtcg gcgtagaggc
      481 agggcccggc gtagtgctcg agcggcacga ggcagcgcgc gagccggctg cggtagacgc
      541 ggtcggcgcg gaagccggcg aacagcgcgg agccgtggcg atagctcagg cccttgtcgg
      601 gatgctccca ggcctggcgg cgcgtccact gcggcccgac gaggcggtgc agcgcgcggc
      661 cggcccgccg caggccggcg agcgggtcgc gggcgcgcac ggtgcggcgg gtgacgtggc
      721 gctcgatcag cgcgtccagc gagaacgcgc gcagcacgat gcgcgggtcg ctcgcgagcc
      781 agcgcagctc ggggccctcg acggtgctcg cgaggtcggt gtccagccac agctcgtagc
      841 gcgcgtgcgg gtggtggtgc agcaggctgc gcacgtgcag ctccgccagc gccggcactc
      901 ggccggccca gtagccgaag aaggtcgggg gcgaggcgag gtcgctcatc gggccccgct
      961 tcattccttc cagtggtcgg cgatccaggg cgtggcgcgc gggctgcgcc agttgccgtt
     1021 gctgcgcccc acgagcgcgt ccggcgggtt gacgacgccg tggaagatga cgatgcgcgc
     1081 gccctcggga atcaccggcg cgcgcacgaa gttcagcggg aagcgcggga tcgagtggta
     1141 cttgaagctc gggcaccact cgtcgggcca gtactgcagc ttgccctgct cgtgcatcac
     1201 ggccgacagg taggcctgct cgttgcggaa gcgcttgcgg atcgtgtcga actccgcgcg
     1261 cagccgggcc agcacctcgg gatgcgcgcc cagctggaag cggtagaccg aggagttgcc
     1321 ggtgacgcgc cagggccgct tccagtcgtg gatgatgcgg aactccccgg gcagctcgaa
     1381 gaagggcgtg atgtcgtcga cgatcacgat gtcgatgtcc aggaacagcg cggtgccgcg
     1441 caggccgtgg aggtcggcgt cgaaggtcag cagcttcttc cacgcgcggt cgggcccctt
     1501 gccctcgacc aggccgagct ccgggatggg caggcattcg acctcgcggc gcacgcctgc
     1561 cgacgaatcg gtcaggcaca cgaagcggaa gggcccgcgc aggtggcggt gtaccattgc
     1621 gtagagccgg ttgacgtact ccggcccgta cttggttccc catttcatgc acaggaccac
     1681 gcggtcggaa gtgcctgctg tcgaagtcgt ctggtccgtc tgcatcggac ggctatcgta
     1741 cccgagcgcc gacggccccc ggcatcgcgc ttgcccgccg gtgcgcccgg ccccgtcccg
     1801 cacccgcctg cgttcccgac cgtgccaccg ctccccgttc ccatgagctc acttttcctt
     1861 cgcaacctcg ccgagcacca ggcgctgatg accgcgctgc cggcactcga cgaggcgatc
     1921 gcggcggccg gacgccgcct ggccgcggcg cttgccgccg gcggcaagat catgttctgc
     1981 ggcaacggcg gctccgcggc cgacagccag cacctcgcct ccgagctcac cggcaggttc
     2041 atcgaggacc gccgcccgct cgcgggcctc gcgctcagca ccgacagctc cgcgctgacg
     2101 tgcatcggca acgactactc gttcgacgac gtcttcgcgc gccaggtggc cggcctcggg
     2161 cgcgcgggcg acgcgctggt cgcgatctcg acctcgggca actcgcgcaa cgtgctgcgc
     2221 gcggtcgccg ccgcgaggca ggcgggcgtc ttcacgctcg gcctgctcgg gcgcgacggc
     2281 ggccagctgc gcgccgcctg cgacgcggcg atcgtcgtgc cgagccaggt caccgcgcgc
     2341 atccaggagg cccacatcct gatcggccac accctgtgcg gcgcgatcga gcacgagctg
     2401 ggcctggtcg aatgagcgcc gcccggccgc ccgaaggcgc tcgcaccgca gtgcgcagca
     2461 cggaggtcgt cgaatgagcg ccgccccggt gctgccgccg ctggccgaac tggaacgccg
     2521 ccgcatcctc gtcgtcggcg acgtgatgct cgatcgctac tggtacggcg ccgtcgagcg
     2581 catctcgccg gaggcgcccg tgccggtcgt gcgcgtcgag cgcgaggagg agcgcctggg
     2641 cggcgccgcc aacgtggcgc tcaacgtcaa gacgctcggc gcgcgcgcga cgctgctgac
     2701 cgtggtcggc caggatcacg ccgccgacgg cctgcgcaag ctgctcggcg cacacggcat
     2761 cgaggccgtg ctgcgcaccg acccgcagct gtcgaccatc gtcaagctgc gcgtgatcgg
     2821 ccgcgcgcag cagctgatcc gcgtcgattt cgagaacgag cccgaccacg aggtgctggg
     2881 gcaaatggtc gacgccttcg ccgagcaact gcccgcgcac gacgtcgtgc tgttctccga
     2941 ctacggcaag ggcggcctcg cgcacatccc gcgcatgatc gagctcgcgc gcgcggcggg
     3001 caagccggtg ctggtcgatc ccaagggcag cgactaccgc cgctacgccg gcgcgagcgt
     3061 cgtgacgccc aaccgcgcgg agctcgcgca ggtcgtgggc acgtggcgcg acgagcgcga
     3121 gctcgcggcc agggtcgcgg ccctgcgcgc ggacatcggc gtcgaggccg tgctgctcac
     3181 gcgctcggag gagggcatga cgctgttcgc cgacggccag acggtgcacg agccggcgcg
     3241 cgcgcgcgag gtcttcgacg tcacgggcgc cggcgacacg gtcatcgcga cgctcgcctc
     3301 gctgctggcc gccggcctgc cgctggacgc ctcgatgcgg ctcgcgaaca aggcgggcgg
     3361 catcgtggtc ggcaagttcg gcacggcgac ggtgagctac gaggagctcg cggcgtgaac
     3421 gtcggctggt cgctcgagga caaggtcttc gacgccgcgg cgctggacga cgcggcgctc
     3481 gccgaccgcc tcgccgcgct gccgcggccg ctggtcttca ccaacggcgt gttcgacgtg
     3541 ctgcaccgcg gccacgtcgc ctacctgcat gcggcccggc ggctcggcgc gagcctggtc
     3601 gtagccgtca acagcgacgc gtcggcccgc ggcctgggca agggccccga ccggccgctg
     3661 aaccgcgacg tcgaccgcgc gctggtgctc gccgggctcg ccgcggtcga cgcggtcacc
     3721 ttcttcgacg aacccacgcc ctgcgcgctg ctcgcgcgcg tgcggccgca gctgtacgtc
     3781 aagggcggcg actacgacat ggagacgctc gaggagaccc gcctcgtgcg ctcctggggc
     3841 ggcgaggcgt tggcgatcgc gttcgtcgag ggctattcga cgaccgcgct cgtgcgccgc
     3901 atccgcgcgg gcgccgcctg atgcggcggg cggccttcct cgaccgcgac ggcgtcgtca
     3961 acgtcgaccg cggctacctg caccgctggg aggacttcga gttcctgccc ggcgccgtcg
     4021 ccgcgatgcg ccggctccag gacgcgggct tcgcgctggt cgtcgtgacc aaccagtcgg
     4081 ggatcgcgcg cggctactac gccgaggacg acttcgtgcg cctgacggac gcgatgtggg
     4141 cggcgctcgt cggcgagggc gtgtcgctgg ccgcggtcga gtggtgcccg caccatcccc
     4201 gcggcagcgt cgcgcgctac gcgatcgcct gcgactgccg caagcccgag cccggcatga
     4261 tcctgcgcgc cgccaaggcg ctggacatcg acctcgccgc gtcggtgctg ttcggcgaca
     4321 agccctccga catcgaggcc gcccgccgcg ccggcgtcgg ccgcgccatc ctcgtcgctc
     4381 ccaacgcggg cgccggctcg ccggacgacg acgaggcggc gatccccgaa ttcgcgagcc
     4441 tggccgaggc ggtggacgcg tacctgggct gacggcccgc cacctcaggc gggcgccgtg
     4501 atcgccgccg gcgccggttc gccgtccagc gcccgcgcga tcacctcgtc ggccgtgatc
     4561 gcggcgaggc tgggcccgcg gatcatgcgc aaccgctgcg gatcgtgctc cagcggcggc
     4621 cgtgggccga ggacgaccca ggtgggcgtg ccgacggccg c
//
LOCUS       DASVLN010000800        12372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_526795, whole genome shotgun sequence.
ACCESSION   DASVLN010000800 DASVLN010000000
VERSION     DASVLN010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12372
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526795"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VF453_18935"
     CDS             complement(<1..210)
                     /locus_tag="VF453_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689789.1"
                     /translation="MSEQVAHAVPADLSALAWVADELRRTLENAHKALRRQQREFETA
                     PPAPDTDGLALPALQQARTLFHQGAG"
     gene            complement(426..3023)
                     /locus_tag="VF453_18940"
     CDS             complement(426..3023)
                     /locus_tag="VF453_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7689790.1"
                     /translation="MSDQSNGSVLKHDGAHTEGGLDNVYPPAPGAAAAVAAHGRGADA
                     ELAVAEELDLSQLGDLGEPEVAHGEHALHASSDGPRPGHREHDTHALHPGAVPTLDID
                     VAELGQSSFPHDGQGPGQGDEGHATTPGGDSSLSIDLPLEHEAATASAALSPVSPEPA
                     PARTIEPEAPAEPAAVPMEDDTGLPLIGRRAIGYQQRVVTGVTAVGVIGLILIGGLAL
                     KAANDHGQQLTATGTALMQSQRLAKSVSQAMIGSPAAFPEVKESAEVLAGDVHGLKSG
                     NDYTPAVGADTQDTVDPMVALVDRASKNANIVLAQQKTLTQVGQALRTINRQSSDLLE
                     TAETVSSLKLQGGASAAEISAVGQLVMLTQRIGKSANEFLTYEGVSPEAVFLLGKDLN
                     TFREIADGLLQGNADLRLPGTKEPATRERLEQLISQYEQTRSQSSAILANLQGLVAAR
                     EAQATIVSDSEPLRKGLEAVQGRLNNVGTVPRAYVIALVASVALLVLGALGILRLFVV
                     GQRQRAALADQQRAEADARQRESQRVNDANQAAILRLMNELQSVAEGDLTQQATVTED
                     ITGAIADSVNYTVEELRTLVAQVQGTAGRVTDTTSQVEATSTELLAASTEQLHEIRET
                     GESVLQMATRINDVSSRAQETLKAAQQSLNVTEVGRKAVQNAIEGMNSIRDQIQETSK
                     RIKRLGESSQEIGEITELISDITEQTNVLALNAAIQAASAGEAGRGFSVVAEEVQRLA
                     ERSGDATRQIAALVKAIQTDTGDAVAAMERSTQGVVQGAQLSDRAGAALVDIDRVTRE
                     LSELVENISTQAQVEAQSANVVASNIQHIFAVTEQTGDGTRSTAQIVRELSRTAEELR
                     QSVSRFKIG"
     gene            complement(3142..3678)
                     /locus_tag="VF453_18945"
     CDS             complement(3142..3678)
                     /locus_tag="VF453_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7689791.1"
                     /translation="MANKEALRELQTRLAERLQAAKTRPRARGWLAVECAGARLLLPL
                     AQAGEIFPLRALLKLPHAKPWMTGVAQLRGDLYTVIDLAAFLGLRAPQGNDASAPDGQ
                     LVMLAPALQVNAALRVDRLAGLRGEEQLAREADAEGEAEQRPAFAAGRWKDAQGATWQ
                     ELDLAALAVDGAFLDVTI"
     gene            complement(3698..4063)
                     /locus_tag="VF453_18950"
     CDS             complement(3698..4063)
                     /locus_tag="VF453_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689792.1"
                     /translation="MPIQHILLVDDSKTELAYLSDLLAKRGFTVRTAENGEEAMKRLA
                     EGKPDLILMDVVMPGTNGYQLTRTITRDPRYADVPVIMCTSKGQETDRVWGLRQGARD
                     YVVKPVDTDDLMAKIKALG"
     gene            complement(4136..4573)
                     /locus_tag="VF453_18955"
     CDS             complement(4136..4573)
                     /locus_tag="VF453_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689793.1"
                     /translation="MADAQTPAAQPGAAAPVRVLVIDDSNTIRRSAEIFLRQGGHEVV
                     LAEDGFDALAKVNDHVPDLIFCDILMPRLDGYQTCAIIKRNARFANVPVIMLSSKDGV
                     FDKARGRMVGSEDYLTKPFTKEQLLRAVAQYQPQPARLSAESQ"
     gene            complement(4616..4789)
                     /locus_tag="VF453_18960"
     CDS             complement(4616..4789)
                     /locus_tag="VF453_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX7689794.1"
                     /translation="MTEPQTWMCLICGWIYDEAAGDAETGLAPGTRWADVPMNWTCPE
                     CGARKEDFEMVRI"
     gene            4916..5872
                     /locus_tag="VF453_18965"
     CDS             4916..5872
                     /locus_tag="VF453_18965"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX7689795.1"
                     /translation="MSTERLPDASGPDDENAEASAPACILTFNASDASGAGGIAGDVA
                     TIAAMGAHALPVVTAVLMRDTAEVFDQHAIDADAVVEQARAILEDITVAGFKVGFLGT
                     TENVTAVAEVVADYPDVPLVSYLPATSWLEDDDQVSYLDAFRELILPATEVLVGNHKT
                     LTDFLLPDWDSDRPASPRELAVAAAEHGTRFVLVTGVMLPAKGGAQYIDNVLASPQGA
                     LTGEKFEMFEASFVGAGDTLSSALASLLGSAAELQAAVGEALQFLDQSLDAGFRPGMG
                     NVVPDRFFWALPPEEGEEGEEAAEGLEPSDDATEIGGSRSVH"
     gene            5862..7160
                     /gene="hemL"
                     /locus_tag="VF453_18970"
     CDS             5862..7160
                     /gene="hemL"
                     /locus_tag="VF453_18970"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX7689796.1"
                     /translation="MSTNAQLFERAQRVIPGGVNSPVRAFRAVGGTPRFIARAEGAYM
                     VDAEGRRYVDYIGSWGPMILGHGHPAVLEAVLAAAREGFSFGAPTEREVELAEEIIRH
                     VPSVEQLRLVSSGTEAAMSAIRLARAATGRSRLVKFEGCYHGHADSLLVKAGSGLATF
                     GHPTSAGVPAEVVQHTTVLDYNDVEGLEAAFAAQGDEIAGVIVEPIAGNMNFVRASVP
                     FMTRLRELCTKHGALLILDEVMTGFRVALGGAQSLYARAIPGFAPDISVFGKVIGGGM
                     PLAAFGASRRIMAQLAPLGPAYQAGTLSGNPVATACGLATLREISRPGFHDALAARTT
                     ALVEGLRVAAADAGVPLAVDSEGGMFGFFFADALPQDYASVLATDKARFNAFFHAMLD
                     AGVYFAPALYEAGFVSAAHGDVEIAHTLQAARAAFAALGN"
     gene            7160..8017
                     /locus_tag="VF453_18975"
     CDS             7160..8017
                     /locus_tag="VF453_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HEX7689797.1"
                     /translation="MAAGTAKLVRVHGQPAVALHAADGARATVLLHGGHVVSWIPAGG
                     EEQLYLSPLTAFGDGASIRGGVPVIFPQFSNRGTLPRHGLARTRGWTLDETPVHAARG
                     EHAQAVLRFVADADTRRLWPHEFEVELTVAVVGRQLDLELVVANTGESPFEFTAALHT
                     YLRTGDVLRAQLEGLQTVKYEDSATGQWQMQWGDVTQVVGALDRIYHDVPAPLTLREA
                     GRRLEVSMSGFTDAVVWNPGPDGARQLSDLPDDDWTRMLCVEAARIIEPVVLPPGEEW
                     AGMQSLHAG"
     gene            8115..9500
                     /gene="mpl"
                     /locus_tag="VF453_18980"
     CDS             8115..9500
                     /gene="mpl"
                     /locus_tag="VF453_18980"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEX7689798.1"
                     /translation="MHIHILGICGTFMGGVAALAREAGHRVTGCDANVYPPMSDQLRA
                     LGIDLIEGYDADQLALAPDLWVVGNAVSRGNPLMEALLDAGLPYTSGPQWLADHVLAG
                     RHVLAVAGTHGKTTTTAMLAWILERAGLEPGFLVGGVPRNFGVSARLGRLHGDGGART
                     PFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEYDHADIFPDLAAIETQFHHLVRTM
                     PGRGRIVVNAREESLQRVLERGCWSEVLRFGARKEEPGALRARGEPHAFDVLRGAMKV
                     ARVEWSLLGEHNQLNALAAIGAAEHVGVAPDAAAAALGSFENVARRLELKGEVNGIRV
                     YDDFAHHPTAIRTTVDGLRRKVGLQQRILAVFEPRSNTMKLGAMKAQLPWALEEADLA
                     FCHSGGLAWDAAEALAPMGAQAQVCDTIAATVAAVARVARPGDHVLCMSNGGFGGIHA
                     KLLAALEAPHT"
     gene            9497..10162
                     /locus_tag="VF453_18985"
     CDS             9497..10162
                     /locus_tag="VF453_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEX7689799.1"
                     /translation="MSGSDAGSPRPAVSGDRPTSASDGRQTITHLLYLHGFRSSPQSA
                     KALWMADWVARHRPDLVWACPQLEPEPAAAMAQARAIVRTWPAATTGVIGSSLGGFYA
                     TALVEETGCRAALVNPAVDPARDLAAHIGRQTLYHAADKSFEFRAEYVDQLAAMTPPR
                     PLAHPRKILAIIAKGDEVLDWREMVGRYAQGPLRLIAGGDHGLTDFDAHAPDLLRHLG
                     LAS"
     gene            10195..10938
                     /gene="fabG"
                     /locus_tag="VF453_18990"
     CDS             10195..10938
                     /gene="fabG"
                     /locus_tag="VF453_18990"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX7689800.1"
                     /translation="MRLAKKIALVTGAAQGIGLATALKFAREGATVVVCDVHDVDAAV
                     ALVSGELADGAACAGHVVDVTDRAQVDAAVAATLARFGRIDVLVNNAGITRDARLQKM
                     TIEQFDAVIDVNLRGVFHCTQAVADAMVAQGSGAILNASSVVGIYGNFGQTNYAATKF
                     GVIGFTKTWSRELGPKGIRVNAVAPGFVETPILATIPEKVLGQMREHVPLKRLGRPEE
                     IANVYAFLASDEASYVNGAVIEVAGGMTV"
     gene            10952..11695
                     /locus_tag="VF453_18995"
     CDS             10952..11695
                     /locus_tag="VF453_18995"
                     /inference="COORDINATES: protein motif:HMM:NF039455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6445 family protein"
                     /protein_id="HEX7689801.1"
                     /translation="MPGALFNPNPKVQAVPIPGGRSCIVIDDALAQPEAWVRFAVAER
                     PHFRNAPANAFPGVERLLPDEFDARLADVFRQHARSALGARRIASLYSRMSMVTLPPR
                     ALQPAQWFCHRDDQAAPAGHMIAACVLYLFHDEALGGTSFWVPRQDAGATQELVLDTC
                     RLSAGEFLRRRAASLGWPADRAPGYVLDSNAAFERVLVVPPRFNRLIFYDGGHYHSGH
                     IAAPERLSDDPTTGRLTINGFITCTRQAA"
     gene            complement(11702..>12372)
                     /locus_tag="VF453_19000"
     CDS             complement(11702..>12372)
                     /locus_tag="VF453_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7689802.1"
                     /translation="AHERGARIASICSGAFLLAAAGLLSGRAATTHWRYAERLQARHP
                     DIEVRPNALYVDEGSIITSAGSAAGLDMMLHLVRRDHGARIANRVAQRLVVPPHRLGD
                     QAQFVERPLPQDEAARVARLMDHVRAHPARDWPLEALAARAHMSVRTLQRQFRQSLGM
                     SPIEWLVRERVELAKQLLEAGRRPLAQIPALAGFGSDESFRRHFRAATGISPIAWRRQ
                     FGAP"
BASE COUNT         1610 a         4570 c         4361 g         1831 t
ORIGIN      
        1 gccggcgccc tggtggaaca gcgtgcgcgc ctgctgcagc gcgggcagcg cgagaccgtc
       61 cgtgtcgggc gcgggcggcg ccgtctcgaa ctcgcgctgc tggcggcgca gggccttgtg
      121 ggcgttctcg agcgtccggc ggagctcgtc ggccacccag gccaacgcgc tcaggtctgc
      181 gggcacggcg tgcgctactt gttcactcaa cgtcatctcc agcgcccgtc ctcgccgaga
      241 agcgagcgca aggggttttc agccgctctc gcaacgccgg agccatcacc cgggacaacg
      301 tgggaatgat gcttcgacgc gtcgtggccc atccaggaag aaacctacgg gggacttccg
      361 ccgcgtgccg cgaggcgccc ttgccgggac gccacgccgc ccgcggcggc catgcctgcc
      421 ggcgatcagc cgatcttgaa ccgcgacacc gactggcgca gttcctcggc cgtccgcgac
      481 agctcgcgca cgatctgcgc cgtggaacgg gtaccgtctc ccgtctgctc cgtcaccgcg
      541 aagatgtgct ggatgttgga ggctacgacg tttgcggact gcgcttcgac ctgagcttgt
      601 gtcgagatgt tctcgaccag ctccgacagc tcgcgcgtca cgcggtcgat gtcgaccagc
      661 gcggcaccgg cgcggtcgga cagctgcgcc ccctgcacca cgccctgggt cgagcgttcc
      721 atggcggcca cggcatcgcc ggtgtcggtc tgaatcgcct tcacgagcgc cgcgatctgg
      781 cgtgtcgcgt cgccggagcg ctcggccagt cgctgcactt cttccgcgac gaccgagaag
      841 ccgcgacccg cctcgccggc cgaggctgcc tggatggcgg cgttcagggc cagcacgttc
      901 gtctgctccg taatgtcgga gatcagctcc gtgatttcgc cgatctcctg cgacgactcg
      961 cccagccgct tgatccgctt ggacgtttcc tggatctggt cgcggatcga gttcatgccc
     1021 tcgatggcgt tctgcaccgc cttgcggccg acttccgtca cgttcaggga ctgctgcgcg
     1081 gccttgagcg tttcctgcgc gcgcgaggac acgtcgttga ttcgggtggc catctgcagc
     1141 acggactcgc cggtctcgcg gatctcgtgc agctgctcgg tcgaggcggc cagcagctcg
     1201 gtggacgtgg cttccacctg gctcgtcgtg tccgtcaccc ggccggccgt gccctgcacc
     1261 tgggcgacga gcgtgcgcag ctcttccacc gtgtagttca ccgagtcggc gatcgcgccc
     1321 gtgatgtcct cggtcaccgt cgcctgctgc gtcaggtcgc cttccgcgac cgactgcagc
     1381 tcgttcatca gccggagaat ggcggcctgg ttggcgtcgt tgacgcgctg cgactcgcgc
     1441 tggcgtgcat ccgcttccgc gcgctgctgg tcggcgaggg ccgcgcgctg gcgctggccg
     1501 acgacgaaca gccgcaggat gcccagcgca cccagcacca gcaacgccac cgacgcgacc
     1561 agcgcgatga cgtaggcgcg cggcaccgtg ccgacgttgt tcagccggcc ctgcacggcc
     1621 tccaggcctt ttcgcagcgg ctcggagtcg gacacgatcg tcgcctgcgc ctcgcgggcg
     1681 gcgaccaggc cctgcaggtt cgcgagaatc gcgctcgact ggctgcgcgt ctgctcgtac
     1741 tgcgagatca gctgttccag gcgctcgcgc gtcgcgggct ccttcgtgcc gggcaggcgc
     1801 aggtcggcgt tgccctgcag caggccgtcg gcgatctcgc ggaaggtgtt caagtccttg
     1861 ccgagcagga acacggcctc ggggctgacg ccttcgtagg tcaggaattc gttggcgctc
     1921 ttgccgatcc gctgcgtcag catcacgagc tggccgaccg cggagatctc ggccgccgag
     1981 gcgccgccct gcagcttcag cgaggagacg gtctcggcgg tctcgagcag gtcggacgac
     2041 tgccggttga tggtgcgcag cgcctggccg acctgggtca gcgtcttctg ctgcgccagc
     2101 acgatgttgg cgttcttcga cgcgcggtcg accagcgcca ccatcgggtc gacggtgtcc
     2161 tgggtgtcgg cgccgacggc cggcgtgtag tcgttgccgg acttcaggcc gtgcacgtcg
     2221 ccggcgagca cttccgcgct ctccttgact tccgggaacg cggcagggct gccgatcatc
     2281 gcctgggaca ccgacttcgc gaggcgctgc gactgcatca gcgcggtacc ggtcgcggtc
     2341 agctgctggc cgtggtcgtt ggcggccttc agcgcgagcc cgccgatcag gatcaggccg
     2401 atcacgccga cggccgtgac gcccgtgacg acgcgctgct ggtagccgat cgcgcggcgg
     2461 ccgatcagcg gcaggccggt gtcgtcttcc atcgggacgg cggcgggctc ggccggcgcc
     2521 tcgggctcga tcgtgcgcgc gggggcgggc tcgggcgaaa ccgggctgag cgcggcgctg
     2581 gccgtcgcgg cctcgtgctc gagcggcagg tcgatcgaca ggctgctgtc gccgccgggc
     2641 gtggtcgcgt ggccctcgtc gccctggccg gggccctggc cgtcgtgcgg gaacgaggac
     2701 tggccgagct cggcgacgtc gatgtccagc gtgggcacgg cgccggggtg cagggcgtgg
     2761 gtgtcgtgct cgcggtggcc gggccgtggg ccatccgacg acgcgtgcag cgcatgctcg
     2821 ccgtgcgcca cctcgggctc gccgaggtcg ccgagctgcg acaggtcgag ctcctcggcg
     2881 acggcgagct ccgcgtcggc gccgcgtcca tgcgcggcca ccgccgcggc ggctcccgga
     2941 gcgggcggat agacattgtc gaggccgccc tcggtgtgcg ccccatcgtg cttcagcacg
     3001 ctgccgttgc tttggtctga caagttcatc tcgccctctt cagctagatt tcgattcccc
     3061 aacacatccc cacgcggccg cgacgccgtc catcgccgtg ccgctctcgc ggcgtcccgt
     3121 cgcgggccac cccactcgca ttcagatcgt cacgtccagg aaggcgccgt cgaccgcgag
     3181 cgcggcgagg tccagttcct gccacgtcgc gccctgcgca tccttccagc ggccggcggc
     3241 aaaggccggc cgttgctcgg cctcgccctc ggcgtcggcc tcgcgcgcca gctgttcctc
     3301 gccgcgcagg ccggcgagcc ggtcgacgcg cagcgcggcg ttgacctgaa gcgcgggcgc
     3361 gagcatcacg agctggccgt ccggcgcgct cgcgtcgttg ccctgcggcg cccgcagccc
     3421 caggaaggcc gcgaggtcga tcacggtgta caggtcgccc cgcagctgcg cgacgcccgt
     3481 catccaaggc ttggcgtgcg gcagcttcag caacgcgcgc agcgggaaga tctcgcccgc
     3541 ctgcgccagc ggcagcagca ggcgcgcgcc ggcgcactcc acggcgagcc agccgcgcgc
     3601 gcgcggacgc gtcttcgcgg cctggaggcg ttccgccagg cgggtctgca gttcgcgcag
     3661 cgcttctttg ttggccatgg gcgtgcgtgc gggggcgtca gcccagggcc ttgatcttgg
     3721 ccatcaggtc gtcggtgtcg accggcttga cgacgtagtc gcgcgccccc tggcgcaggc
     3781 cccagacgcg gtccgtctcc tggcccttgc tggtgcacat gatcaccggc acgtcggcgt
     3841 agcgcgggtc gcgcgtgatg gtgcgcgtca gctggtagcc gttggtgccg ggcatgacca
     3901 cgtccatcag gatcaggtcg ggcttgccct cggcgagccg cttcatggcc tcctcgccgt
     3961 tctccgcggt gcgcaccgtg aacccgcgct tggcgagcag gtccgagaga taggccagtt
     4021 cggtcttcga gtcgtcgacc aggagaatgt gctggatcgg catgatggat gggtcctctt
     4081 gcgtcgggag cgtcgggggc cggttgcggc gtcgcccgtc cgcgtccctg ggctgttatt
     4141 gcgattcggc ggacagcctg gcgggctgcg gctggtactg ggccaccgcg cgcagcagct
     4201 gttccttcgt gaagggcttg gtcaggtagt cctcggagcc gaccatgcgc ccgcgcgcct
     4261 tgtcgaacac gccgtccttg gaggacagca tgatcaccgg cacgttggcg aagcgggcgt
     4321 tgcgcttgat gatcgcgcag gtctggtagc cgtccagccg cggcatcagg atgtcgcaga
     4381 agatcaggtc gggcacgtgg tcgttgacct tggcgagcgc gtcgaagccg tcctccgcga
     4441 gcacgacctc gtggccgccc tggcgcagga agatctcggc gctgcgccgg atcgtgttgc
     4501 tgtcgtcgat caccagcacg cgcaccggcg ccgccgcgcc cggctgggcg gcgggggtct
     4561 gggcgtcagc catgggtgac ctcgcttgcg ggatgcgcca tcgtgcggcc ttccatcaga
     4621 tgcggaccat ctcgaagtct tccttgcgcg cgccgcactc cgggcaggtc cagttcatgg
     4681 ggacgtccgc ccagcgggtg ccgggcgcca gaccggtttc ggcatcgccg gcagcctcgt
     4741 cgtagatcca gccacagatc aggcacatcc aggtttgggg ttcggtcacg ggtttcgcgc
     4801 agaaagggtc actacaatcg accgcattgt atgcatgccc cgaagccttc cgagccgctt
     4861 cagaggccaa aagagtcaca ttttccgggc cttccaggcc tgatcggcac cgaacatgtc
     4921 cactgaacgc cttcccgacg catccgggcc ggacgacgag aacgccgagg ccagcgcgcc
     4981 ggcgtgcatc ctcaccttca acgcgagcga cgccagcggc gccggcggca tcgccggcga
     5041 cgtcgccacg atcgccgcga tgggcgccca cgccctgccc gtggtcacgg ccgtgctgat
     5101 gcgcgacacg gccgaggtct tcgaccagca cgccatcgac gccgatgcgg tcgtcgagca
     5161 ggcgcgcgcc atcctcgagg acatcaccgt cgccggcttc aaggtcggct tcctcggcac
     5221 gacggagaac gtcaccgccg tcgccgaggt cgtcgccgac tacccggacg tcccgctcgt
     5281 gtcctacctg ccggccacgt cctggctgga ggacgacgac caggtctcct atctcgacgc
     5341 cttccgcgag ctgatcctgc cggccaccga ggtgctggtc ggcaaccaca agacgctgac
     5401 cgacttcctg ctgcccgact gggattccga ccgcccggcc tcgccgcgcg agctcgccgt
     5461 tgccgccgcc gagcacggca cccgcttcgt gctggtcacg ggcgtgatgc tgccggccaa
     5521 gggcggggcg cagtacatcg acaacgtgct cgcgtcgccg cagggcgcgc tcacgggcga
     5581 gaagttcgag atgttcgagg cgagcttcgt cggcgccggc gacacgctgt cgtccgcgct
     5641 cgcgtcgctg ctgggctcgg ccgccgagct ccaggcggcc gtcggcgagg cgctgcagtt
     5701 cctcgaccag agcctggacg ccggcttccg ccccggcatg ggcaatgtcg tccccgaccg
     5761 cttcttctgg gccctgccgc ccgaggaggg cgaggaaggc gaggaggccg ccgagggcct
     5821 cgagccctcc gacgacgcga ccgagatcgg gggctcgcgc agtgtccact aatgcccagc
     5881 tgttcgagcg cgcccagcgc gtgatcccgg gcggcgtgaa ttcgccggtg cgcgccttcc
     5941 gcgccgtcgg cggcacgccg cgcttcatcg cccgcgccga gggcgcctac atggtcgatg
     6001 ccgagggcag gcgctacgtg gactacatcg gctcctgggg cccgatgatc ctcggccacg
     6061 gccacccggc ggtgctcgag gccgtgctgg ccgccgcgcg cgagggcttc agcttcggcg
     6121 cgccgacgga gcgcgaggtg gagctggccg aggagatcat ccgccacgtc ccgagcgtcg
     6181 agcagctgcg gctggtgagc tcgggcaccg aggccgcgat gagcgcgatc cgcctcgcgc
     6241 gcgcggcgac cgggcgctcg cgcctcgtca agttcgaggg ctgctaccac ggccacgcgg
     6301 actcgctgct cgtgaaggcg ggctcgggcc tcgcgacctt cggccacccg acctccgccg
     6361 gcgtccccgc cgaggtcgtg cagcatacga cggtgctgga ctacaacgac gtcgagggcc
     6421 tcgaggccgc gttcgccgcc cagggcgacg agatcgccgg cgtgatcgtc gagccgatcg
     6481 ccggcaacat gaacttcgtg cgcgcgagcg tgcccttcat gacgcgcctg cgcgagctct
     6541 gcacgaagca cggcgcgctg ctgatcctcg acgaggtgat gacgggcttc cgggtcgcgc
     6601 tcggcggcgc gcagtcgctg tacgcgaggg cgatcccggg cttcgcgccg gacatcagcg
     6661 tcttcggcaa ggtgatcggc ggcggcatgc cgctggcggc cttcggcgcg tcgcgccgga
     6721 tcatggcgca gctcgcgccg ctcgggccgg cgtaccaggc cggcacgctg tcgggcaacc
     6781 cggtggcaac cgcctgcggc ctcgcgacgc tgcgcgagat ctcgcggccc ggcttccacg
     6841 acgcgctcgc cgcgcgcacg accgcgctcg tcgaagggct gcgcgtcgcg gcggcggacg
     6901 cgggcgtccc gctcgcggtc gacagcgagg gcggcatgtt cgggttcttc ttcgccgacg
     6961 cgctgccgca ggactacgcg agcgtcctcg ccaccgacaa ggcgcgcttc aacgcgttct
     7021 tccatgcgat gctggacgcg ggcgtgtact tcgcgcccgc gctgtacgag gcgggcttcg
     7081 tgagcgccgc ccacggcgac gtcgagatcg cgcacacgct gcaggccgcg cgcgccgcgt
     7141 tcgcggccct ggggaactga tggcggcggg caccgcgaag ctcgtgcggg tgcacggcca
     7201 gccggccgtc gcgctgcacg ccgccgacgg cgcgcgcgcg acggtgctgc tgcacggcgg
     7261 ccacgtcgtg agctggatcc ccgccggcgg cgaggagcag ctctacctct cgccgctgac
     7321 ggcgttcggc gacggcgcgt cgatccgcgg cggcgtgccg gtgatcttcc cgcagttctc
     7381 caaccgcggc acgctgcccc ggcacggcct cgcgcgcacg cgtgggtgga cgctcgacga
     7441 gacgcccgtg cacgccgcgc gaggcgagca tgcgcaggcg gtgctgcgct tcgtcgccga
     7501 cgcggacacg cgccggctgt ggccgcacga gttcgaggtc gagctcaccg tcgccgtcgt
     7561 cggccgccag ctcgacctgg agctggtcgt cgccaacacc ggcgagtcgc ccttcgagtt
     7621 caccgccgcg ctgcacacct acctgcgcac cggcgacgtc ctccgtgcgc agctggaagg
     7681 cctgcagacc gtcaagtacg aggacagcgc gaccggccag tggcagatgc agtggggcga
     7741 cgtcacgcag gtcgtgggcg cgctcgaccg catctaccac gacgtccctg ccccgctcac
     7801 gctgcgcgag gccgggcggc ggctcgaggt ctcgatgagc ggcttcaccg acgcggtcgt
     7861 ctggaacccc ggccccgacg gcgcgcgcca gctgtccgac ctgcccgacg acgattggac
     7921 gcgcatgctg tgcgtcgagg ccgcgcgcat catcgagccg gtcgtcctgc cgccgggcga
     7981 ggaatgggcc ggcatgcaga gcctgcacgc cggctgaccg acaatcgggg tcccgcgatc
     8041 gcgaccccac ggcgcggggc cggcgcgaat cgtgcatgcc cgcggcgcgt ccgaacgagc
     8101 acgagtcctt ttccatgcac atccacatcc tcggcatctg cggcaccttc atgggcggcg
     8161 tggcggcgct cgcgcgggag gccggccacc gcgtgacggg ctgcgacgcc aacgtgtatc
     8221 cgccgatgag cgaccagctg cgcgcgctcg gcatcgacct gatcgaaggc tacgacgccg
     8281 accagctcgc gctcgcgccc gacctctggg tcgtcggcaa cgccgtctcg cgcggcaacc
     8341 cgctgatgga ggcgctgctg gacgcgggcc tgccctacac gagcggcccc caatggctcg
     8401 ccgaccacgt gctcgccggc cgccacgtgc tggccgtggc cggcacgcac ggcaagacga
     8461 cgacgaccgc gatgctcgcc tggatcctgg agcgcgccgg cctcgagccg ggcttcctgg
     8521 tcggcggcgt gccgcgcaac ttcggcgtct cggcccggct cggccggctg cacggcgacg
     8581 gcggcgcccg cacgcccttc gtcatcgagg ccgacgagta cgacaccgcg ttcttcgaca
     8641 agcgcagcaa gttcgtgcac taccggccgc gcaccgcggt gctgaacaac ctcgagtacg
     8701 accacgcgga catcttcccc gacctcgcag cgatcgagac ccagttccac cacctcgtgc
     8761 gcaccatgcc cgggcgcggc cgcatcgtcg tcaacgcgcg cgaggagtcg ctgcagcgcg
     8821 tgctggagcg cggctgctgg agcgaggtgc tgcgcttcgg agcgcgcaag gaggagcccg
     8881 gcgccctgcg cgcccgcggc gagccgcatg ccttcgacgt gctgcgcggc gcgatgaagg
     8941 tcgcgcgcgt cgagtggtcg ctgctgggcg agcacaacca gctcaacgcc ctggccgcga
     9001 tcggcgccgc cgagcacgtg ggcgtggcac ccgacgcggc ggctgcggcg ctgggctcgt
     9061 tcgagaacgt cgcgcgccgc ctcgagctga agggcgaggt caacggcatc cgtgtctacg
     9121 acgacttcgc gcaccatccg accgcgatcc gcaccacggt ggacggcctg cgccgcaagg
     9181 tcggcctgca gcagcgcatc ctcgccgtgt tcgagccccg atccaacacg atgaagctgg
     9241 gcgcgatgaa ggcccagctg ccgtgggcgc tcgaggaggc cgacctcgcg ttctgccact
     9301 cgggcggcct cgcctgggac gccgccgagg cgctcgcgcc gatgggggcg caggcgcagg
     9361 tctgcgacac gatcgcggcg acggtcgcgg cggtcgcgcg cgtcgcccgg cccggcgacc
     9421 acgtcctgtg catgagcaac ggcggcttcg gcggcatcca cgccaagctc ctcgccgcgc
     9481 tcgaggcgcc gcacacgtga gcggatccga cgccggctcg ccgcggccag ccgtctccgg
     9541 cgatcggccg acgtccgcgt cggacgggcg gcagacgatc acgcacctgc tgtacctgca
     9601 cggcttccgc tcgtcgccgc aatcggccaa ggcgctgtgg atggccgact gggtcgcgcg
     9661 ccaccggccc gacctcgtgt gggcctgccc gcagctcgag cccgagccgg ccgccgcgat
     9721 ggcgcaggcg cgcgcgatcg tgcgcacctg gccggcggcg accaccggcg tcatcggcag
     9781 ctcgctgggc ggcttctacg ccaccgcgct ggtggaggag accggctgcc gtgccgcgct
     9841 cgtcaacccc gcggtcgatc ccgcgcgcga cctcgcggcc cacatcggtc gccagaccct
     9901 ctatcacgcc gcggacaaga gcttcgagtt ccgcgccgag tatgtcgacc agctcgccgc
     9961 gatgaccccg ccgcgcccgc tcgcccatcc gcgcaagatc ctcgcgatca tcgccaaggg
    10021 cgacgaggtg ctggactggc gcgagatggt gggccgctac gcgcagggcc cgctcaggct
    10081 gatcgccggc ggcgaccacg gcctcaccga cttcgacgcc cacgcccccg acctgctgcg
    10141 ccacctcggg ctggcgtcct gacccgatac cccgttttcg taccaatcca cccgatgcgc
    10201 ctcgccaaga agatcgccct cgtcaccggt gccgcccagg gcatcggcct cgccaccgcc
    10261 ctgaagttcg cgcgcgaagg cgccaccgtc gtcgtctgcg acgtgcacga cgtcgacgcc
    10321 gcggtcgccc tcgtctccgg agagctcgcc gacggcgccg cctgcgcggg gcacgtcgtc
    10381 gacgtgaccg accgcgcgca ggtcgacgcc gcggtggccg cgacgctggc gcgcttcggc
    10441 cgcatcgacg tgctcgtgaa caatgccggc atcacccgcg acgcgcgcct gcagaagatg
    10501 acgatcgagc agttcgacgc cgtcatcgac gtgaacctgc gcggcgtctt ccactgcacg
    10561 caggcggtcg ccgacgcgat ggtggcgcag ggctcgggcg cgatcctcaa cgcgagctcg
    10621 gtcgtgggca tctacggcaa cttcggccag accaactacg ccgccaccaa gttcggcgtc
    10681 atcggcttca ccaagacctg gagccgcgag ctcgggccca agggcatccg cgtcaacgcg
    10741 gtcgcgccag gcttcgtcga gacgcccatc ctcgcgacca ttcccgagaa ggtgctcggc
    10801 cagatgcgcg agcacgtgcc gttgaagcgc ctgggccggc ccgaggagat cgccaacgtc
    10861 tacgccttcc tcgcgagcga cgaggcgagc tacgtcaacg gcgcggtgat cgaggtcgcg
    10921 ggcggcatga ccgtctgatc cgcggcgacg catgcccggg gcgctcttca acccgaaccc
    10981 gaaggtgcag gcggtgccca tcccgggcgg ccgctcctgc atcgtgatcg acgacgcgct
    11041 cgcccagccc gaggcctggg tgcgcttcgc ggtcgccgag cgcccccact tccgcaacgc
    11101 gcccgccaac gcgttcccgg gcgtggagcg cctgctgccc gacgagttcg acgcccgcct
    11161 cgccgacgtc tttcgccagc acgcacgctc cgcgctcggc gcgcgccgga tcgcgagcct
    11221 gtacagccgg atgtcgatgg tgacgctgcc gccgcgggcg ctgcagcctg cgcagtggtt
    11281 ctgccatcgc gacgaccagg ccgcgcctgc cggccacatg atcgcggcct gcgtgctgta
    11341 cctcttccac gacgaggcgc tcggcggcac cagcttctgg gtgccgcgcc aggacgcggg
    11401 cgcgacgcag gagctggtgc tcgatacctg ccgcctgtcc gccggcgagt tcctgcgccg
    11461 ccgcgccgcg tcgctgggct ggccggccga ccgcgcaccg ggctacgtgc tcgactccaa
    11521 cgccgccttc gagcgcgtgc tggtcgtgcc gccgcgcttc aaccggctca tcttctacga
    11581 cggcggccac taccactcgg gccacatcgc cgcgccggag cgcctgagcg acgacccgac
    11641 caccggccgg ctgacgatca acggcttcat cacctgcacc cggcaggcgg cctgaccgcc
    11701 gtcacggcgc gccgaactgg cgccgccacg cgatcgggct gatgcccgtg gcggcgcgga
    11761 aatggcggcg gaacgactcg tcggagccga acccggccag cgccgggatc tgcgcgagcg
    11821 gccgccggcc ggcctcgagc agctgcttgg caagctcgac gcgctcgcgc accagccatt
    11881 cgatcggcga catcccgagc gactgccgga actgccgctg cagcgtgcgc acgctcatgt
    11941 gcgcgcgggc cgccagcgcc tcgagcggcc agtcgcgcgc cggatgcgcg cgcacgtggt
    12001 ccatcaggcg ggcgacgcgg gcggcctcgt cctgcggcag cgggcgttcg acgaactgcg
    12061 cctggtcgcc cagccggtgc ggcggcacga ccagccgctg cgcgacgcgg ttggcgatgc
    12121 gcgcgccgtg gtcgcgccgc acgaggtgca gcatcatgtc cagccccgcc gcggagccgg
    12181 ccgaggtgat gatcgagcct tcgtcgacgt acagcgcgtt cggccgcacc tcgatgtcgg
    12241 gatggcgcgc ctgcaggcgc tcggcgtagc gccagtgcgt ggtcgccgcg cggcccgaga
    12301 gcagccccgc cgccgccagc aggaacgcgc ccgagcagat cgacgcgatg cgcgcgccgc
    12361 gctcgtgcgc cg
//
LOCUS       DASVLN010000801         4836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_363478, whole genome shotgun sequence.
ACCESSION   DASVLN010000801 DASVLN010000000
VERSION     DASVLN010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363478"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /gene="trpS"
                     /locus_tag="VF453_19005"
     CDS             complement(<1..601)
                     /gene="trpS"
                     /locus_tag="VF453_19005"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7689803.1"
                     /translation="MRARVLSGMRPTGALHLGHYHGALKNWVRLQHDYDSFFFVADWH
                     ALTTHYEDREVIERNVYDMVVDWIAAGLDPDLCTIFIQSRLPEHAELFALLAMGTPVG
                     WLERVPTYKDQIGQLPGRDLATYGFLGYPLLQAADILIYKAAWVPVGEDQDAHVELTR
                     EVAGRFNHLYGRSPRQQAALAAALARLPVDAAAEVARLHR"
     gene            692..1342
                     /gene="purN"
                     /locus_tag="VF453_19010"
     CDS             692..1342
                     /gene="purN"
                     /locus_tag="VF453_19010"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX7689804.1"
                     /translation="MKRIVVLVSGQGSNLDAIVRASIAQDWPGRVVAVLSNRPDAGGL
                     ARAQAHGIATAALDHRSFPDRLAFDAALGAEIDRHDPDLVVMAGFMRILSPEFVARYQ
                     GRMLNIHPSLLPSFTGLHTHRRAIEAGCKLAGATVHFVGAELDHGPIVIQAAVPVRAG
                     DDEAALARRVLAVEHVIYPRAVGWFVRGEISLQGGHVMHRDNAPQWLMVDEPDPAA"
     gene            1434..2699
                     /locus_tag="VF453_19015"
     CDS             1434..2699
                     /locus_tag="VF453_19015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX7689805.1"
                     /translation="MHPNALLDLATDLLREVGKLDMPADVVVSNFFRKHRQLGPRERH
                     ALAETAYAVLRQRLLWQHLAQSGHGALERRLAILAWQGGETLIRGGMGPQEQQWLNQV
                     RALDRGAFSEKLRHNLPDWLAGALKAELGDEGFWDFVASMSGQAPLDLRVNLLKTGRE
                     KAQAQLAAAEVQAVPTPYSPWGLRVDGKPALQKSDVFLRGDIEVQDEGSQLLAALVNP
                     KRGEMVVDFCAGAGGKTLALGAAMRNTGRLYAFDVNGHRLDKLKPRLARSGLSNVHPA
                     QLTNERDERVKRLAGKIDRVLVDAPCSGLGTLRRNPDLKWRLSAKAIDELVELQGRIL
                     DSACRLLKPGGRLVYATCSPLRREDEAIAEAFGAAHPQFERLDAAELLKSAGVERADE
                     LVVDGYFRSWPHRHGMDGFFAAAWQKSAA"
     gene            2889..4112
                     /locus_tag="VF453_19020"
     CDS             2889..4112
                     /locus_tag="VF453_19020"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX7689806.1"
                     /translation="MIDFQSVLDGFVSFFSTGLLHATWWEIVLYTLVTTHITIAAVTI
                     FLHRAQAHRALDLHAIPSHFFRFWLWVGTGMVTKEWVAIHRKHHAKCETVDDPHSPQV
                     HGIKKVLLEGVELYRAESKVKETMTKYGHNTPDDWVERNVYSRYQWQGVALLMILNLV
                     LFGAIGATVWAVQMLWIPITAAGVINGIGHYWGYRNFEATDASRNVLPWGILIGGEEL
                     HNNHHTYPTSAKLSVKPYEFDIAWGYIRAMEMVGLAKVRKTPPQLRMGHVRPVADNET
                     LEAIIANRYEVMAKYAREMRRACAAEMARLRADRAANGSKLANLRLARRWLHRDIEKI
                     PGHLQATIAATAAESPQLSKLVTMREELRALWTRTNVSAEQAVSDLQAWCKQAEESGI
                     EALREFSMKLRAARA"
     gene            4341..>4836
                     /locus_tag="VF453_19025"
     CDS             4341..>4836
                     /locus_tag="VF453_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7689807.1"
                     /translation="MRFDLVTLNLVLAIAETRSITRGAEREHLALAAASKRLSDLEAR
                     LGVALFDRRARGVDLTEAGRALVRHIRTLNASLHALESEVVEFSRGIKGHLRVVANAG
                     AISECLPADLAAFARAHPGIRVSLEDQTSADVQQAVAEGRADIGIFVAPGTDSRLATR
                     HYSSG"
BASE COUNT          734 a         1753 c         1658 g          691 t
ORIGIN      
        1 ggcggtgcag gcgcgcgacc tccgcggccg cgtcgaccgg caggcgcgcg agtgccgccg
       61 ccagcgccgc ctgctgccgc ggcgagcggc cgtagaggtg gttgaagcgc cccgcgacct
      121 cgcgcgtgag ctcgacgtgc gcgtcctggt cctcgcccac cggcacccag gcggccttgt
      181 agatcaggat gtcggcggcc tgcagcagcg ggtagccgag gaagccgtag gtcgcgaggt
      241 cgcggccggg cagctggccg atctggtcct tgtaggtcgg cacgcgctcc agccagccca
      301 ccggcgtgcc catcgcgagc agggcgaaca gctcggcgtg ctcgggcagc cggctctgga
      361 tgaagatcgt gcagagatcg gggtcgaggc cggcggcgat ccagtcgacc accatgtcgt
      421 agacgttgcg ctcgatgacc tcgcgatcct cgtagtgcgt cgtcagggcg tgccagtccg
      481 cgacgaagaa gaagctgtcg tagtcgtgct gcagccgcac ccagttcttc agggcgccgt
      541 ggtagtggcc gaggtgcagc gccccggtgg gacgcatgcc ggacaggacg cgggctcgca
      601 tggcggtacg gtcgggagcg tgaggcggaa ccggcgattg taggcaggcg gcggccgggc
      661 tgtccgcgat gggccggtag aatcgcggcc catgaaaaga atagtggtcc tggtctcggg
      721 ccagggctcg aacctggacg cgatcgtgcg cgcgtcgatc gcgcaggact ggcccggccg
      781 cgtggtcgct gtcctcagca accgtccgga cgccggcggc ctcgcgcgcg cgcaggcgca
      841 cggcatcgcc accgcggcgc tggaccacag gtccttcccc gatcgcctgg ccttcgacgc
      901 cgcgctgggc gccgagatcg accgccacga ccccgacctc gtcgtgatgg cgggcttcat
      961 gcgcatcctg tcgccggagt tcgtcgcgcg ctaccagggc cggatgctca acatccatcc
     1021 gtcgctgctg ccatcgttca ccgggctgca cacgcatcgg cgcgcgatcg aggctggctg
     1081 caagctcgcg ggcgcgaccg tgcacttcgt cggtgccgag ctcgaccacg gtccgatcgt
     1141 gatccaggcc gcggtgccgg tgcgcgccgg cgacgacgag gccgcgctcg cccgccgcgt
     1201 gctggcggtc gagcacgtga tctacccgcg cgcggtcggc tggttcgtcc gcggcgagat
     1261 ctccctacag ggcggccacg tcatgcaccg cgacaatgcg ccccaatggc tgatggtcga
     1321 cgagcccgat ccggccgcct gaccacgcca tcccccgatc cgcccctcct gccccagcgg
     1381 cgccgcgcgc cgtccgggcc cgatttctgc gacaaccgcg aaagtccgcc gccatgcacc
     1441 cgaacgccct cctcgacctc gccaccgacc tgctgcgcga ggtcggcaag ctcgacatgc
     1501 cggccgacgt cgtggtgtcc aacttcttcc gcaagcaccg ccagctgggg ccgcgcgagc
     1561 gccacgcgct ggccgagacc gcctacgccg tgctgcgcca gcggctgctc tggcagcatc
     1621 tcgcgcagag cggccacggc gcgctcgagc ggcgcctggc gatcctcgca tggcagggcg
     1681 gcgagacgct gatccgcggc ggcatggggc cgcaggagca gcagtggctc aaccaggtgc
     1741 gcgcgctcga ccgcggcgcc ttctccgaga agctgcgcca caacctgccc gactggctgg
     1801 ccggcgcgct caaggccgag ctcggcgacg agggcttctg ggacttcgtc gcgtcgatgt
     1861 ccgggcaggc gccgctcgac ctgcgcgtga acctgctcaa gaccggccgc gagaaggcgc
     1921 aggcccagct cgccgcggcc gaggtccagg cagtcccgac gccgtactcg ccctggggcc
     1981 tgcgcgtcga cggcaagccg gcgctgcaga agtccgacgt cttcctgcgc ggcgacatcg
     2041 aggtgcagga cgagggcagc cagctgctgg cggcgctcgt caaccccaag cgcggcgaga
     2101 tggtcgtcga cttctgcgcc ggcgccggcg gcaagacgct ggcgctgggc gcggcgatgc
     2161 gcaacacagg gcggctctac gccttcgacg tcaacggcca ccggctggac aagctcaagc
     2221 cgcggctcgc gcgcagcggg ttgtccaacg tccacccggc gcagctgacc aacgagcgcg
     2281 acgaacgcgt caagcgcctg gccggcaaga tcgaccgcgt gctggtcgac gcgccgtgct
     2341 cgggcctggg cacgctgcgt cgcaatcccg acctgaagtg gcggctgtcg gccaaggcga
     2401 tcgacgagct cgtcgagctg cagggccgca tcctcgacag cgcctgccgc ctgctgaagc
     2461 cgggcggccg gctcgtctac gcgacctgca gcccgctgcg gcgcgaggac gaggcgatcg
     2521 ccgaggcctt cggcgccgcg catccgcagt tcgagcgcct ggacgcggcg gagctgctga
     2581 agtcggccgg cgtcgagcgc gcggacgagc ttgtcgtcga cggctacttc cgcagctggc
     2641 cgcatcgcca cgggatggac ggcttcttcg ccgcggcctg gcagaaatcc gcagcgtgat
     2701 ggtcgaccat tcgaaaaaac cgcatcgcgg gccgcggccg tgctgaactt cacgcgcttc
     2761 ggatgctcga aaggctccgc gttgccaagg aatgcacgtt cttggcggcg catttcggta
     2821 ggcgcgcgca ggcgcatccc ccctacaatt cgtcaccgcc cggtttttcg ggcaccacag
     2881 gcagggcaat gatcgacttc caatcggttc tcgacggttt cgtgagtttc ttctccaccg
     2941 ggctgctgca cgcgacctgg tgggagatcg tcctgtacac gctggtgacg acgcacatca
     3001 cgatcgcggc ggtcacgatc ttcctgcacc gcgcgcaggc gcaccgcgcg ctcgacctgc
     3061 acgcgatccc ctcgcacttc ttccgcttct ggctgtgggt gggcaccggc atggtgacca
     3121 aggagtgggt cgcgatccac cgcaagcacc acgccaagtg cgagacggtc gacgacccgc
     3181 acagcccgca ggtccacggc atcaagaagg tgctgctgga aggcgtcgag ctctaccgtg
     3241 ccgaatccaa ggtcaaggag acgatgacca agtacggcca caacacgccg gacgactggg
     3301 tcgagcgcaa cgtgtacagc cgctaccagt ggcagggcgt ggcgctgctg atgatcctga
     3361 acctggtgct gttcggcgcg atcggcgcga ccgtgtgggc cgtgcagatg ctgtggatcc
     3421 cgatcaccgc cgccggcgtc atcaacggca tcggccacta ctggggctac cgcaacttcg
     3481 aggccaccga cgcctctcgc aacgtgctgc cctggggcat cctgatcggc ggcgaggagc
     3541 tgcacaacaa ccaccacacc tatcccacct cggccaagct gtccgtcaag ccctacgagt
     3601 tcgacattgc ctggggctac atccgcgcga tggagatggt cggcctggcc aaggtgcgca
     3661 agacgccgcc gcagctgcgc atgggccacg tccgccccgt cgccgacaac gagacgctgg
     3721 aggcgatcat cgccaaccgc tacgaggtga tggccaagta cgcccgcgag atgcgccgcg
     3781 cgtgcgctgc cgagatggcc cgcctgcgcg ccgaccgcgc ggccaacggc tcgaagctgg
     3841 ccaacctgcg cctcgcgcgc cgctggctgc atcgcgacat cgagaagatc ccgggccacc
     3901 tgcaggccac gatcgccgcc accgccgccg agtcgccgca gctgtccaag ctggtgacca
     3961 tgcgcgagga gctgcgcgcc ctgtggacgc gcaccaacgt ctcggccgaa caggccgtct
     4021 ccgacctgca ggcctggtgc aagcaggccg aggaatcggg catcgaggcc ctgcgcgagt
     4081 tctcgatgaa gctgcgcgcc gcccgcgcct gacgcgtccc ccgcccgtcc gaagagcccg
     4141 cagccgcggg cttttttcat ggcgtcggcg tttcgagggg ccttgccggt cgacggttcg
     4201 tcgattacga tggcttcgca ccaaacgaag gattgcgaag caactaccga agtgcggatt
     4261 cgcgagcctt cgcgcatcat acgcatcggg tcgctcgtgg atcctgcccc gccgtccacg
     4321 cccgccatcc gaccgccgcc atgcgcttcg acctcgtcac cctcaacctc gtcctcgcga
     4381 tcgccgagac ccgcagcatc acccgcggcg ccgaacgcga gcacctggcg ctggccgcgg
     4441 ccagcaagcg gctgtccgac ctcgaggcgc gactgggcgt cgcgttgttc gatcgccgcg
     4501 cccgaggcgt cgacctgacc gaggccggtc gcgcgctggt ccgccacatc cgcacgctca
     4561 acgcctcgct gcacgcgctg gagagcgagg tcgtcgagtt ctcgcgcggc atcaaggggc
     4621 atctgcgggt ggtggccaac gcgggcgcga tctcggaatg cctgccggcc gacctcgccg
     4681 ccttcgcgcg cgcgcacccc ggcatccgcg tgagcctgga agaccagacg agcgccgacg
     4741 tgcagcaggc cgtggccgag ggtcgcgccg acatcggcat cttcgtcgcc ccgggcacgg
     4801 actcgcgcct ggcgacgcgc cactacagca gcggca
//
LOCUS       DASVLN010000802        12091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_256869, whole genome shotgun sequence.
ACCESSION   DASVLN010000802 DASVLN010000000
VERSION     DASVLN010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256869"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VF453_19030"
     CDS             <1..391
                     /locus_tag="VF453_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7689808.1"
                     /translation="EPDARLARALQALQAPAGPPAAPLRWIRASAGELMHQVDVADVL
                     FFHADDKYTCVRTARAEHLIRTPIAELAAQLDAREFQQVHRSTIVNLRHLAGTRRDDA
                     SRLFVRVRGWDGELPVSRAYVHLFKPM"
     gene            complement(425..1516)
                     /locus_tag="VF453_19035"
     CDS             complement(425..1516)
                     /locus_tag="VF453_19035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF025874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HEX7689809.1"
                     /translation="MVDWNRKFRSEYDAARAVLMAGDGFDPAWRPLVEQLAALMAWDG
                     FDVAREDALAQLRRRLLDEGQPGRQADELALLEAAGGAADGASLSPDESRRIAALKLL
                     RHVYLQSRAGNRRVWIVSLPTEFTNWPSRQFVDEAGSAARVQRLAASRREHFNEEQRR
                     LLGAATQQGLAWCQRAGMVLANTSQRGRGGAEARALVRRWFAEDGVPDAVLEALAGTL
                     ARGFKEIVATLNRGHFVVTDWVPFRAATSADESEFLRTEAFAFRARSEGLDVVYVEGA
                     FFDDLGGNVLRGLANWTRILVHELSHLVCGTHDVNNGQARYAWCGIGPHAGNPHVDTI
                     RNADNWAFFAADCAGALAPGQRETALRRT"
     gene            complement(1689..2090)
                     /locus_tag="VF453_19040"
     CDS             complement(1689..2090)
                     /locus_tag="VF453_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689810.1"
                     /translation="MSHDPSRPPPPVVVPLEHDGIRYMPDEHPPVGPGQIPGGYLAAF
                     DARTGAKLWTALIYASHEDPAAPFQPGRHFRALNLSVDGQGIDVEDEYGVHYAFDFET
                     RTARRATPATPPQVMRFVRPLPVPPKAPGAS"
     gene            complement(2183..2311)
                     /locus_tag="VF453_19045"
     CDS             complement(2183..2311)
                     /locus_tag="VF453_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689811.1"
                     /translation="MGDVFFLSMKLDAPRRAAIVIGSARGDRFTYHIDSGTVRQSP"
     gene            complement(2346..2702)
                     /locus_tag="VF453_19050"
     CDS             complement(2346..2702)
                     /locus_tag="VF453_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689812.1"
                     /translation="MPPPGMPRASRRAPPEVPPVEHDGVLYEPLASRDVSEVDPQTVY
                     VVARDAATRTPLWWTPLYRIELVPHLERDVQMVFIRSLALSEDGHRLLAENSRGRRFE
                     IDIASHEVHELPAAPR"
     gene            complement(2834..3916)
                     /locus_tag="VF453_19055"
     CDS             complement(2834..3916)
                     /locus_tag="VF453_19055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF025874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HEX7689813.1"
                     /translation="MAWWDKPFTPEYDAVQSAIGAKGFSAPFSKLQAELAKGVGATGF
                     EPSGAQAFDALRSWVSTPRPGEPPSADEATRILDAASASPLLDPDERRRAAAIKTLRH
                     VYMAQQRGGHSSWIVSLPLDFRQWPREAFRLASPDAVKALLRSNNERFSKIDIDNLSA
                     GMLSALRWSQKAQVVLGALNGSGQAKADALDLLKTWFGDGIASDAQVAALAPTLRMGF
                     NIITAALNRNQVILTDFVPIRGAAYPSDEYDFLTSEAFVFGLSGRERLDVIYVEEDFF
                     NAGNVLKGQANWARILVHEMSHLCVGTDDMGQGAQTRYCGYGIKPNANFPMAEAIRNA
                     ENWAFFAADCAGALGAGEKARALRVI"
     gene            complement(4130..6853)
                     /gene="tssH"
                     /locus_tag="VF453_19060"
     CDS             complement(4130..6853)
                     /gene="tssH"
                     /locus_tag="VF453_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX7689814.1"
                     /translation="MSEISRVALFGKLNPMAYKGVEGATVFCKLRGNPYVELVHWLHQ
                     LIQNTDSDLHRIFKHYALDASRLASDLTTALDGLPRGATAISDFSPVIEEMIQEAWLY
                     GTLQYGESAVRTGLLMVGGLKKPSLRNALYALSGEFRKIKPDDFTDNFAKIVNGSPED
                     TLAANDGSNVGGGAAPGEASGAMAPAQMGKQEALKKFTVDLTAQARSGKMDPIVGRDD
                     EIRQVIDILMRRRQNNPILVGEAGVGKTAVVEGFAQRIVRGDVPPSLKDVQLLALDVG
                     LLQAGASMKGEFEQRLRSVIDEVQASPKPIILFIDETHTLVGAGGAAGTGDAANLLKP
                     ALARGQLRTVGATTWAEYKKHIEKDPALTRRFQNIQVDEPSEAKAILMMRGVASTMEK
                     HHKVQILDEALEAAVRLSHRYIPARQLPDKSVSLLDTAAARVAISLHAVPAEVDDSQR
                     RIEALETETQIIARESAIGIDTRERDEAVKAALVAERERLAALTQRWNDEKALVDRLL
                     SLRAKLRDGGHSVEGTGSTLEASADKAADAKATLSDGERADLTSELRGVQDELAKLQG
                     ENPLILPTVDFQAVASVVGDWTGIPVGRMAANEIDTVLKLPRLLGARVIGQDHAMEMI
                     SKRIQTSRAGLDNPNKPIGVFMLAGTSGVGKTETALALAEALYGGEQNLITINMSEYQ
                     EAHTVSSLKGAPPGYVGYGEGGVLTEAVRRKPYSVVLLDEVEKAHSDVHELFFQVFDK
                     GWMEDGEGRQIDFKNTLILLTTNAGTEMIANLCKDPDLMPDPEGMAKAIREPLLKIFP
                     PALLGRLVTIPYYPLSDEMLGKIVGLQLGRIKKRVEARYKMPFTYSDDVVKLVVSRCT
                     ESESGGRMIDAILTNTMLPEISREFLTRMMEGKAIERVNVGVKDGDFSYEF"
     gene            complement(6979..8148)
                     /gene="tssG"
                     /locus_tag="VF453_19065"
     CDS             complement(6979..8148)
                     /gene="tssG"
                     /locus_tag="VF453_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX7689815.1"
                     /translation="MSDVSDRDADDDSESTRLMSVADVLGAIDVVKSAVAPSAFAVAD
                     MLARKEAAAAERYEARKQLLQALTETPFKFGFFQSVRLLENAHPGLPRIGTSLRLRDD
                     PIRFAQSPSLAFAPSTLAGFTPGEGNAAPTLTVRFFGLFGANGPLPLHLTEFARERAR
                     RAPADRALVRFLDMFHHRMMSLLYRAWAESQPTVSLDRPDNDPFSRWVGSVAGYGLSS
                     LRTRDSVPDGARLAVAGILGRAVHGAEGLERILNDFFRVRVRVHQWEPHWMRLPEEAR
                     SRVGLRANPVALGENAVVGARVWDCQTRFRVEIGPLTLAEYERFLPGGESMRRLRDWV
                     LNYVGYELSCEMHLVLKREEVPAVRLGGAGRLGWTSWIGRRRESTDATDLRLGIT"
     gene            complement(8443..10329)
                     /gene="tssF"
                     /locus_tag="VF453_19070"
     CDS             complement(8443..10329)
                     /gene="tssF"
                     /locus_tag="VF453_19070"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX7689816.1"
                     /translation="MDSRLLGYYRQELLHIRDAAAEFAREHPKIAARLALDGTVAGGE
                     CVDPYVERLLEGFAFLAARVHYQQDSEYAHFTQHLLELVYPGYLAPTPSMLIAQMEPD
                     LDDPSLAVGPEVPRGSVMSSRLQPGERTACRFTTGQAVRLWPLRIAQARFQSHVTDVP
                     APALSGIVSPNACLRVRFKVGASLLCKDLELDELVLHLSGDDGIAHRVYEQLFARCHA
                     IAVVEPLKRHALVGVLGADALVAGGFEDDEALLPALDRSLAGYRLLKEYSAFPARYLF
                     ARLRGLRQLLARGTTSEFELLFITDRSDTTLEKSLDASSFALHCTPAINLFRKRADRV
                     EINTRDHEFHVIADRARPIDYEVHSLVSVQGIDKSGVTQVREFKPLYARYDDADSDAY
                     AYFSTRRIQRVVSDATRARGPRSGYIGTELYITLADPKTAPWPSSVVQLAIDTLSSNR
                     DLPLTLSVGSANDFFLEENTPVKGVRCIRGPTRPIAPIGEGQAPWRALSHLSLNYLSL
                     IDSEGGGGAAALRELLSLYGLDELSPLHKQLEGLVSVRSEAAISRVPVKGPIVFGRGT
                     KVKLAMDERAFEGTGIVVLGAVLDRFIARYASMNSFTQLVLTSQTRGVIKTWPPRIGA
                     RSVL"
     gene            complement(10352..10849)
                     /gene="tssE"
                     /locus_tag="VF453_19075"
     CDS             complement(10352..10849)
                     /gene="tssE"
                     /locus_tag="VF453_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEX7689817.1"
                     /translation="MAEALSRDRLYPSLLDRLIDDEPGKLTEPRENRAASLQKLRDNV
                     LRDLNWLFNAVQSSESLNDDELLTGSVINYGLPPLAGRPASQYEVTELSRVLRDVILR
                     FEPRIIAHTLKVRAEKMSADSHNVLGFRIEGQLWSQPVPLEIFMRTEIDLESGMTNVM
                     ETKAP"
     gene            complement(10867..11709)
                     /locus_tag="VF453_19080"
     CDS             complement(10867..11709)
                     /locus_tag="VF453_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEX7689818.1"
                     /translation="MQFSSADPLAHFDDLRRMIQKQPQRSDLRIFLFQLYCVQGEWVK
                     AATQLDVLLELDPSSKPMVETYREALRCEVLRRDVFDGKRSPLVLGKPQDWLAGMLEA
                     LRLDAEGHRDAATDLRARSLEAAPATAGTLNDEPFAWLADADGRLGPVVEAIINGKYY
                     WVPVSRLMRVEIEKPADLRDFVWAPATLTLANGAANVALIPTRYPGTEREADGPLRLA
                     RATDWREQPGGAWHGVGQRMLTTDQTEIALLDVRTVTLECEPEAPATAVEPGEGGDVA
                     DAAA"
     gene            complement(11896..>12091)
                     /locus_tag="VF453_19085"
     CDS             complement(11896..>12091)
                     /locus_tag="VF453_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEX7689819.1"
                     /translation="LKITMTEVLVTSVSQGGSGGEDRLTENVTLNFASVKVDYVEQTP
                     KGGTGDKPSMNWNIAENSKD"
BASE COUNT         1744 a         4213 c         4270 g         1864 t
ORIGIN      
        1 ggagcccgac gcgcgcctcg cccgcgcact gcaggccttg caggcaccgg ccgggccgcc
       61 cgcggccccg ctgcgctgga tccgcgccag cgccggcgag ctgatgcacc aggtcgacgt
      121 cgcggacgtg ctgttcttcc acgccgacga caagtacacc tgcgtgcgca cggcgcgcgc
      181 cgagcacctg atccgtaccc cgatcgccga gctcgcggcg cagctggatg cgcgcgagtt
      241 ccagcaggtg caccggtcga ccatcgtcaa cctccgccac ctcgccggca cgcgccgcga
      301 cgacgcgagc cggctgttcg tgcgcgtgcg cggctgggac ggcgagctgc ccgtcagccg
      361 cgcctacgtg cacctgttca agccgatgtg agacgggtcg cctggatacg gcaccccgta
      421 tccttcaggt acgccgcagc gccgtctcgc gctggcccgg ggcgagcgca cccgcgcagt
      481 cggccgcgaa gaacgcccag ttgtcggcat tgcggatggt gtcgacgtgc gggttgccgg
      541 catgcggccc gatgccgcac caggcatagc gggcctggcc gttgttgacg tcgtgcgtgc
      601 cgcagacgag gtggctcagc tcgtgcacca ggatgcgcgt ccagttcgcg aggccgcgca
      661 gcacgttgcc gcccaggtcg tcgaagaacg ccccctcgac gtagacgacg tccaggccct
      721 cgctcctggc acgaaaggcg aaggcctcgg tgcgcaggaa ctcggactcg tcggcgctcg
      781 tcgcggcgcg gaacggcacc cagtcggtga cgacgaagtg gccgcggttc agcgtcgcga
      841 cgatctcctt gaagccgcgc gccagcgtgc cggcgagcgc ctccagcact gcgtccggga
      901 cgccgtcctc cgcgaaccac cgccgcacca gggcccgtgc ctcggcgccg ccacgcccgc
      961 gctgcgacgt gttggcgagc accatgccgg ctcgctggca ccacgcgagg ccctgctggg
     1021 tcgccgcgcc gagcaggcgc cgctgctcct cgttgaaatg ctcgcgccgg ctggcggcca
     1081 gccgctgcac gcgcgcggcg ctgcccgcct cgtcgacgaa ctggcggctc ggccagttgg
     1141 tgaactccgt gggcaacgac acgatccaca cccgccggtt gccggcgcgg ctttgcaggt
     1201 agacgtggcg cagcagcttc agcgcggcga tgcggcgcga ctcgtcgggc gacagcgagg
     1261 cgccgtccgc ggccccgccc gcggcctcga gcagcgcgag ctcgtcggcc tggcggccgg
     1321 gctgcccctc gtcgagcagg cgccggcgca gctgggcgag cgcgtcctcg cgcgcgacgt
     1381 cgaagccgtc ccacgccatc agcgcggcca gctgctcgac gagcgggcgc cacgccggat
     1441 cgaacccgtc gccggccatc agcacggcgc gggcggcgtc gtattcgctc ctgaatttcc
     1501 tgttccagtc gaccatcgga tgcctgtgct cgccggctct cgcgggcgcg aggccctacg
     1561 atacgcgaag ccgcgcgggc ggcccatccc gagggcgggg gctcggcgcc cgattcggcg
     1621 tgaaccgggc gtgtcgccat cagtccggcg tccagtgcgt ttggccggat ctacccaggg
     1681 cacgcccctc agctcgcgcc cggcgccttc ggcggcacag gcagcggtcg cacgaagcgc
     1741 atgacttgcg gaggcgtcgc cggcgtcgcg cggcgggctg tgcgggtctc gaagtcgaac
     1801 gcatagtgca cgccgtattc atcttcgacg tcgatgccct gcccgtcgac cgacaggttc
     1861 agcgccctga agtggcgccc tggctggaac ggggcggctg gatcctcatg gctcgcgtag
     1921 atgagcgccg tccatagctt cgcaccggtc ctcgcgtcga aagcggccag gtagccgcct
     1981 gggatctggc cgggcccgac cggagggtgt tcgtccggca tgtagcgaat accgtcgtgc
     2041 tcgaggggca cgactaccgg cggcggtggc cggctggggt cgtggctcat cgaagattgt
     2101 tccttggaca cacggcaatg cgaagcgaga ctggcgtccc atcccgatgg gaggacggcc
     2161 gaccgcggcg ggcgcccgtt ggtcatggcg actgccgcac cgtgccgctg tcgatgtgat
     2221 atgtgaagcg gtcgccccgc gcgcttccga tcacgatcgc ggcgcggcgc ggcgcgtcga
     2281 gcttcatgga caggaagaac acgtcgccca cgtcgagctc gatccagccg ccagcgcccg
     2341 agcagtcagc gcggcgcagc gggcagttcg tgaacctcgt gcgacgcgat gtcgatctcg
     2401 aatcgcctgc cgcgggaatt ttcggcaagg agccggtgac catcctcgga cagcgcgagg
     2461 ctgcgaatga agaccatctg cacatcccgc tcgagatgcg ggacgagttc gatgcggtag
     2521 agcggggtcc accacagcgg cgtgcgtgtc gcagcgtctc gcgcgacgac gtagaccgtc
     2581 tgcggatcga cctcggagac gtcgcggctg gccagcggct cgtataggac gccatcatgt
     2641 tcgacgggcg gcacttcggg tggcgcgcgg cgactggcgc ggggcatacc gggtggaggc
     2701 atggcggaat tcctgttcgt tgatgaggcg gttggcgtcc gggcggccgc cagatgaaag
     2761 gccgctaagg ctgcgaccgt ggttccgacc gaccacgccg ccgcgacgcg acgatgtcgg
     2821 cgcgccatcg gtctcagatc acgcgcaggg cccgggcctt ttcgcctgcg cccagcgcgc
     2881 cggcgcagtc ggcagcgaag aaggcccagt tctccgcatt gcggatcgcc tcggccatcg
     2941 ggaaattggc gttgggcttg atgccgtagc cgcaatagcg ggtctgggcg ccctggccca
     3001 tgtcgtccgt cccgacgcac agatgagaca tttcgtgcac gaggatgcgc gcccagttcg
     3061 cctgcccctt caacacgttg ccggcgttga agaagtcctc ttcgacatag atgacatcca
     3121 gacgctccct gccagacagg ccgaagacga acgcttccga cgtgagaaag tcgtattcgt
     3181 ccgacgggta ggcggcaccc cgaataggca cgaagtcggt cagtatcacc tggttgcgat
     3241 tgagcgccgc cgtgatgata ttgaagccca tgcgcagcgt cggcgccagc gcggcgacct
     3301 gcgcgtcgct ggcaatgccg tcgccaaacc atgtcttgag caagtcgagc gcgtccgcct
     3361 tggcttggcc cgatccgttc agcgccccca gcacgacctg cgccttctgg ctccagcgca
     3421 acgcggacag catgcctgcc gacaggttgt cgatatcgat cttcgagaag cgttcgttgt
     3481 tgcttctcaa cagtgccttg actgcgtcgg gcgaggcgag tcgaaacgcc tcacgcggcc
     3541 actggcggaa gtcgagcggc aacgagacga tccagctgct gtgtccgccg cgctgctggg
     3601 ccatgtagac atgccgcagc gtcttgatgg ccgcggcgcg acggcgctcg tccgggtcga
     3661 gcaacggcga cgcggaggcg gcatccagaa tccgcgtcgc ctcgtcggcc gacggcggct
     3721 cgccagggcg cggcgtgctg acccaactgc gcaaggcgtc gaacgcctgc gcgcccgagg
     3781 gctcgaagcc cgtggcaccg acccccttgg ccaactcggc ctgcagcttc gagaacggtg
     3841 ccgagaaacc tttcgcgccg atcgcggatt gcaccgcgtc gtactccggg gtgaacggct
     3901 tgtcccacca ggccatgtct ctgagctcct gttctcgcgg attccaggca tgcaccgatg
     3961 ggtgcgcgcc gccgcgacca ccggccacga tatgcgaaga cagcttgctt cgcgcccctc
     4021 gaacgggggg ggccagcccg gctggacagc cgactgccgt agaaagcaac acgccgcgca
     4081 ggctgcagga gcctgcacgg cgcatgtgca aatgggtcgc tgcgcgggct cagaactcgt
     4141 acgagaagtc cccatccttc acgccgacgt tcacgcgctc gatggccttg ccttccatca
     4201 tgcgggtgag gaactcgcgg ctgatctcgg gcagcatcgt gttggtcagg atcgcgtcga
     4261 tcatgcggcc gcccgactcg ctctcggtgc agcggctcac gaccagcttg acgacgtcgt
     4321 ccgagtaggt gaacggcatc ttgtagcgcg cctcgacgcg cttcttgatg cgacccagct
     4381 gcagcccgac gatcttgccc agcatctcgt cggacagcgg gtagtacggg atcgtgacga
     4441 ggcggcccag cagcgcgggc gggaagatct tgagcagcgg ctcgcggatc gccttggcca
     4501 tgccctcggg gtcgggcatg aggtcgggat ccttgcagag gttggcgatc atctcggtgc
     4561 cggcgttggt cgtcagcagg atgagcgtgt tcttgaagtc gatctggcgg ccctcgccgt
     4621 cctccatcca gcccttgtcg aagacctgga agaacagctc gtgcacgtcg gagtgcgcct
     4681 tctcgacctc gtcgagcagc acgacgctgt acggcttgcg gcgcaccgcc tcggtcagca
     4741 cgccgccctc gccgtagccg acataccccg ggggggcacc cttgagcgag gagacggtgt
     4801 gcgcctcctg gtactcgctc atgttgatcg tgatgaggtt ctgctcgccg ccgtacagcg
     4861 cctcggccag cgcgagcgcg gtctcggtct tgccgacgcc cgaggtgccg gccagcatga
     4921 acacgccgat cggcttgttc gggttgtcca ggccggcgcg cgaggtctgg atgcgcttgg
     4981 agatcatctc catcgcgtgg tcctggccga tcacgcgcgc accgagcagg cgcggcagct
     5041 tcagcacggt gtcgatctcg ttggcggcca tgcgaccgac cgggatgccg gtccagtcgc
     5101 cgacgacgct cgccacggcc tggaagtcca ccgtcggcag gatcagcggg ttctcgccct
     5161 gcagcttggc cagctcgtcc tgcacgccgc gcagctcgga ggtcaggtcg gcgcgctcgc
     5221 cgtcgctgag cgtcgccttc gcgtcggcgg ccttgtcggc gctcgcctcc agcgtgctgc
     5281 cggtgccctc gacggaatgg ccgccgtcgc gcagcttggc gcgcagggag agcagccggt
     5341 ccaccagcgc cttctcgtca ttccagcgct gggtcagcgc ggccaggcgc tcgcgttcgg
     5401 cgacgagcgc cgccttcacg gcctcgtcgc gctcgcgcgt gtcgatgccg atcgcggact
     5461 cgcgggcgat gatctgggtc tcggtctcga gcgcctcgat ccggcgctgc gagtcgtcga
     5521 cctcggccgg caccgcatgc aggctgatcg cgacgcgcgc ggcggccgtg tcgagcaggc
     5581 tgaccgactt gtccggcagt tgccgcgccg ggatgtagcg atgcgacagc ctgaccgcgg
     5641 cctcgagcgc ctcgtcgagg atctgcacct tgtggtgctt ctccatcgtg ctggcgacgc
     5701 cgcgcatcat caggatggcc ttggcctcgc tcggctcgtc gacctggatg ttctggaagc
     5761 ggcgggtcag cgccggatcc ttctcgatgt gcttcttgta ctcggcccac gtcgtggcgc
     5821 cgaccgtacg cagctggccg cgcgcgagcg ccggcttgag caggttggcc gcgtcgccgg
     5881 tgccggcggc gccgcccgcg ccgaccagcg tgtgggtctc gtcgatgaac aggatgatgg
     5941 gcttggggct cgcctgcacc tcgtcgatga ccgagcgcag gcgctgctcg aactcgccct
     6001 tcatgctcgc gccggcctgc agcaggccga cgtccagcgc gagcagttgc acgtccttca
     6061 ggctgggcgg cacgtcgccg cgcacgatgc gctgggcgaa gccctcgacc accgccgtct
     6121 tgccgacgcc ggcctcgccg accaggatcg ggttgttctg gcgccggcgc atcaggatgt
     6181 cgatgacctg gcggatctcg tcgtcgcggc cgacgatcgg atccatcttg ccgctcctgg
     6241 cctgcgccgt gaggtcgacc gtgaacttct tgagcgcctc ctgcttgccc atctgggccg
     6301 gggccatcgc gccgctcgcc tcgcccggcg ccgcgccgcc gccgacgttg gagccgtcgt
     6361 tggctgcgag cgtgtcctcc ggcgagccgt tgacgatctt ggcgaagttg tcggtgaagt
     6421 cgtccggctt gatcttgcgg aattcgccgg acagcgcgta cagcgcgttg cgcagcgagg
     6481 gcttcttcag gccgccgacc atcagcaggc ccgtgcgcac ggcgctctcg ccgtactgca
     6541 gcgtgccgta cagccaggcc tcctggatca tctcctcgat cacgggcgag aagtccgaga
     6601 tcgccgtcgc gccacgcggc aggccgtcca gcgcggtcgt caggtcggag gccaggcgcg
     6661 acgcatctag cgcgtagtgc ttgaagatgc ggtgcaggtc gctgtccgtg ttctggatca
     6721 gctggtgcag ccagtgcacc agctccacgt acgggttgcc ccgaagcttg cagaagaccg
     6781 tggcgccttc cacgcccttg taggccatcg gattgagctt gccgaacaag gcgacgcgac
     6841 tgatttcact cacggggaca ctcctctgtg ggcgctgggt gcgccgaatt cgaatctgtt
     6901 ggtaacgaac gcattggagc cgcgatcgac ccgcatttca ataccctctt gcgcggggtc
     6961 gcggcgccgt cgtcgcgctc aggtgatgcc caggcgcagg tcggtcgcgt cggtcgactc
     7021 gcgccggcgc ccgatccatg acgtccaccc cagccggccg gcgccgccga gccgcaccgc
     7081 gggcacctcc tcgcgcttga ggacgaggtg catctcgcag gacagctcgt agccgacgta
     7141 gttcagcacc cagtcgcgca ggcggcgcat gctctcgcct ccgggcagga agcgctcgta
     7201 ctcggcgagg gtcagcgggc cgatctcgac gcggaagcgc gtctggcagt cccacacgcg
     7261 cgcgccgacg acggcgttct cgccgagcgc caccgggttc gcccgcaggc cgacgcgcga
     7321 gcgtgcctcc tcgggcaggc gcatccagtg cggctcccat tggtggacgc gcacgcgcac
     7381 ccggaagaag tcgttgagga tgcgttccag gccctcggcg ccgtgcacgg cgcgccccag
     7441 gatgcccgcc accgccagcc gcgcgccgtc cggcaccgag tcccgcgtcc tgagcgagct
     7501 gaggccgtag ccggcgacgg agccgaccca gcggctgaac gggtcgttgt ccgggcggtc
     7561 gaggctcacc gtcggctggg actcggccca ggcacggtac agcagcgaca tcatccggtg
     7621 gtggaacatg tccaggaagc gcaccagcgc gcgatcggcc ggcgcgcggc gcgcgcgctc
     7681 gcgcgcgaac tccgtcaggt gcagcggcaa cgggccgttg gcgccgaaca gcccgaagaa
     7741 gcgcacggtc agcgtcggcg ccgcgttgcc ctcgcccggc gtgaatcccg cgagcgtcga
     7801 cggcgcgaac gcgagcgatg gcgactgcgc gaagcggatc ggatcgtcgc gcagccgcag
     7861 cgaggtgccg atccgcggca gtcccggatg cgcattctcc agcaggcgca cggactggaa
     7921 gaagccgaac ttgaacgggg tctcggtcag cgcctgcagc agttgcttgc gcgcctcgta
     7981 gcgttcggcg gccgcggcct ccttgcgggc cagcatgtcg gccaccgcga acgcgctggg
     8041 cgcgaccgcc gacttcacga cgtcgatcgc gccgagcacg tcggcgacgc tcatcagccg
     8101 cgtcgactcc gagtcgtcgt ccgcatcgcg atccgacacg tcactcacag caagcctccg
     8161 agccggcgcc cggcagggcg cacggcccaa gcggcagccg cggatccggc ttcgccgggc
     8221 cgctgggtgc gccccctcgg gggaggcgcc gcaggcgctt cggggggagc gacatgtctg
     8281 cagccgcgga accggcttcg ccgggccgct ggctgcgccc cctcgggggg aggcgccgaa
     8341 ggcgcttcgg ggggagcgac atgtccgcag ccgcggaacc ggcttcgccg ggccgctggg
     8401 tgcgccccct cggggggagg cgccgcgggc gcttcggggg ggtcacagga ccgaacgggc
     8461 gccgatgcgc ggcggccagg tcttgatcac gccccgcgtc tgcgaggtca gcaccagctg
     8521 cgtgaagctg ttcatgctgg cgtagcgcgc gatgaagcga tcgagcaccg cgcccagcac
     8581 cacgatgccg gtaccctcga atgcgcgctc gtccatcgcc agcttcacct tggtgccgcg
     8641 gccgaagacg atcgggccct tgaccgggac gcggctgatc gccgcctcgc tgcgcaccga
     8701 gaccagcccc tcgagctgct tgtgcagcgg cgagagctcg tccaggccat acagcgacag
     8761 cagctcgcgc aacgccgccg cgccaccgcc gccttcgctg tcgatcagcg acaggtagtt
     8821 cagcgacagg tggctcaacg cgcgccaggg cgcctggccc tcgccgatcg gggcgatcgg
     8881 gcgcgtcggc ccgcggatgc accgcacgcc cttgaccggc gtgttctcct cgaggaagaa
     8941 gtcgttggcg ctgcccaccg acagcgtcag cggcaggtcg cggttggacg acagcgtgtc
     9001 gatcgcgagc tgcaccaccg agctcggcca cggcgcggtc ttcgggtcgg ccagcgtgat
     9061 gtacagctcg gtgccgatgt agccgctgcg cgggccgcgc gcgcgcgtcg cgtccgacac
     9121 cacgcgctgg atgcggcgcg tcgagaagta ggcgtaggcg tcgctgtcgg cgtcgtcata
     9181 gcgcgcgtac agcggcttga actcacgcac ctgcgtgacg ccggacttgt cgatgccctg
     9241 cacagagacc agcgaatgca cctcgtaatc gatcggccgc gcgcgatcgg cgatgacgtg
     9301 gaactcgtgg tcgcgggtgt tgatctcgac gcggtcggcg cgcttgcgaa acaggttgat
     9361 cgccggcgtg cagtgcagcg cgaagctgct cgcgtcgagc gacttctcga gcgtcgtgtc
     9421 gctgcggtcg gtgatgaaca gcagctcgaa ctcggacgtc gtgccccggg ctagcagctg
     9481 gcgcaggccg cgcaggcgcg cgaacaggta gcgcgcgggg aacgcggaat actccttcag
     9541 cagccggtag ccggcgagcg agcggtcgag cgccggcagc agcgcctcgt cgtcctcgaa
     9601 gcccccggcc accagcgcgt ccgcccccag cacgccgacc agcgcatggc gcttgagcgg
     9661 ctcgaccacc gcgatcgcat ggcagcgcgc gaacagctgc tcgtagaccc gatgcgcgat
     9721 gccgtcgtcg cccgacaggt gaagcacgag ctcgtcgagc tccagatcct tgcacagcag
     9781 ggacgcgccg accttgaagc gcacgcgcag gcaggcgttg ggcgagacga tgcccgacag
     9841 cgccggcgcc ggcacgtcgg tcacgtgcga ctggaagcgc gcctgcgcga tccgcagcgg
     9901 ccacaggcgc acggcctggc cggtcgtgaa gcggcaggcg gtgcgttcgc ccggctgcag
     9961 gcgcgagctc atgacgctgc cgcgcggcac ctcggggccg acggcgagag acgggtcgtc
    10021 gagatccggc tccatctgcg cgatcagcat cgacggcgtc ggcgcgaggt agccggggta
    10081 gaccagctcc agcaggtgct gcgtgaagtg cgcgtactcc gagtcctgct ggtagtgcac
    10141 gcgcgccgcc aggaaggcga aaccctccag caggcgctcg acgtagggat cgacgcattc
    10201 gccgccggcc acggtgccgt ccagcgcgag gcgggcggcg atcttcggat gctcgcgcgc
    10261 gaactccgcc gcggcgtcgc ggatgtgcag cagttcctgc cggtagtagc cgaggagtcg
    10321 ggaatccatg ggcgatctcg gacggcaccg ctcagggcgc cttggtctcc atgacgttgg
    10381 tcatgccgct ctccaggtcg atctccgtgc gcatgaagat ctccagcggc accggctgcg
    10441 accagagctg gccctcgatg cggaagccga ggacgttgtg cgagtcggcc gacatcttct
    10501 ccgcgcgcac cttgagcgtg tgcgcgatga tgcgcggctc gaagcgcagg atcacgtcgc
    10561 gcagcacgcg cgagagctcg gtgacctcgt actgcgaggc ggggcggccg gccagcggcg
    10621 gcaggccgta gttgatgacc gagccggtga gcagctcgtc gtcgttcagg ctctcgctgc
    10681 tctggacggc attgaacagc cagttcaggt cccgcaggac gttgtcgcgc agcttctgca
    10741 gcgacgcggc gcgattctcg cgcggctccg tgagcttgcc cggctcgtcg tcgatcaggc
    10801 ggtcgaggag cgacgggtag agccggtcgc gcgagagcgc ttcagccatg ggcggttctc
    10861 agcctctcac gcggcggcat cggcgacgtc gccgccctcg cccggctcga cggccgtcgc
    10921 cggtgcctcg ggctcgcact ccagcgtgac ggtgcgcacg tcgagcaagg cgatctcggt
    10981 ctggtcggtg gtcagcatgc gctggccgac gccgtgccag gcgccaccgg gctgctcacg
    11041 ccagtcggtc gcgcgcgcga ggcgcagcgg gccgtcggcc tcgcgctcgg tgccgggata
    11101 gcgggtcggg atcagagcga cgttcgcggc gccgttggcc agcgtcagcg tcgcgggcgc
    11161 ccacacgaag tcgcgcaggt cggcgggctt ctcgatctcg acgcgcatca ggcgcgagac
    11221 cggcacccag tagtacttgc cgttgatgat cgcctcgacg accgggccga ggcggccgtc
    11281 ggcatcggcc agccaggcaa agggctcgtc gttgagcgtg ccggcggtcg cgggcgcggc
    11341 ctcgagcgag cgggcgcgca ggtcggtcgc ggcatcgcgg tgaccttcgg catcgaggcg
    11401 cagcgcctcc agcatgccgg cgagccagtc ctgcggcttg cccagcacca gcggcgagcg
    11461 cttgccgtcg aagacgtcgc gccgcagcac ctcgcagcgc agcgcctcgc ggtaggtctc
    11521 gaccatcggc ttggaggacg ggtccagctc cagcagcacg tccagctgcg tggccgcctt
    11581 cacccattcg ccctgcacgc agtacagctg gaacaggaag atgcgcaggt cggagcgttg
    11641 cggctgcttc tggatcatcc gacgcaggtc gtcgaaatgc gccaggggat cggcggagga
    11701 aaactgcatc gcgagatcga tggacgccat gaacactcac cccgagggtc gaaaggcaga
    11761 cgccggcatc gctgccggcg tccgtgggta tgcgggtctt ccgggacggg ggacggcagc
    11821 cggctgcggt ccggcccgcc gtgtcgcccg gaagacgacg agccgacccg cgggcgggcc
    11881 ggcgtcgctc ggaactcagt ccttcgagtt ctcggcgatg ttccagttca tcgacggctt
    11941 gtcgcccgtg ccgcccttcg gggtctgctc gacgtagtcg accttgaccg aggcgaagtt
    12001 cagcgtgacg ttctccgtca ggcggtcctc gccgcccgag ccgccttgcg acaccgaggt
    12061 cacgaggact tcggtcatcg tgatcttcag g
//
LOCUS       DASVLN010000803         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_526865, whole genome shotgun sequence.
ACCESSION   DASVLN010000803 DASVLN010000000
VERSION     DASVLN010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526865"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VF453_19090"
     CDS             <1..1447
                     /locus_tag="VF453_19090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7689820.1"
                     /translation="AEAAANEALAHFRVTGSARGQAFALNALGIVAWRRGVPSRVLEF
                     TGEAVVLARAAEEPVLQVRIANTLGAVLNDIGRLPESVQILEEGLRASRGFPDEAVVL
                     RLRANLALALARWALREFDERLPESAWRPRAERAIEVMRPAIDAWRERGRPELLSNVL
                     ENLAVAHIVLGRLEDAHATLDEAERLMDPASARHSIVFFSCVRARAHLQAGEPLLALQ
                     AVERGRAAVDARGGHIYMDEVHRLKSLAHEARGELREALDAYKRFHELRTRLVLERAE
                     QQASALAITLNTERALRQVSEERDRAERAHREARHDQLTGLANRRRFDEYLAGMLPRA
                     TDEQPISLVLIDLDHFKSINDRYRHLAGDAALRWVARHLQAQCRQVDLPARLGGDEFA
                     LVLNAPLAVAHQVCTRLRVAVAGRSSELPSDVTIRLSAGIAEATAPCDPQHLFRRADE
                     ALYAAKAGGRDGVRHDGPGAGVRRTDKEYRP"
BASE COUNT          213 a          608 c          585 g          192 t
ORIGIN      
        1 cgccgaggcc gcggccaacg aggcgctcgc gcatttccgc gtgacgggct cggcgcgcgg
       61 ccaggcgttc gccctcaacg cgctgggcat cgtcgcctgg cgccgcggcg tgccgtcgcg
      121 ggtgctggag ttcaccggcg aggcggtggt gctcgcgcgc gcggcggagg agccggtgct
      181 gcaggtgcgc atcgccaaca cgctgggcgc ggtgctcaac gacatcgggc ggctgccgga
      241 atcggtgcag atcctcgagg agggcctgcg cgcgtcgcgc ggcttcccgg acgaggccgt
      301 cgtgctgcgc ctgcgggcca acctggcgct cgcactggcc cgctgggcgc tgcgcgagtt
      361 cgatgaacgc ctgcccgaat cggcctggcg ccctcgcgcc gagcgcgcga tcgaggtgat
      421 gcgtccggcg atcgacgcct ggcgcgagcg cggccgcccc gagctgctga gcaacgtgct
      481 cgagaacctg gccgtcgccc acatcgtgct cggccggctc gaagacgcgc acgcgacgct
      541 ggacgaggcc gagcgactca tggatccggc ctcggcgcgc cactcgatcg tcttcttcag
      601 ctgcgtgcgc gcccgcgcgc acctgcaggc cggcgagccg ctgctcgcgc tgcaggcggt
      661 cgagcgcggc cgcgccgccg tcgacgcgcg cggcggccac atctacatgg acgaggtgca
      721 tcgcctgaag tcgctcgccc acgaggcgcg cggcgagctg cgcgaggcgc tcgacgccta
      781 caagcgcttc cacgagctgc gcacgcggct ggtgctggag cgcgcggagc agcaggcgag
      841 cgcgctcgcg atcacgctca acaccgagcg cgcgctgcgc caggtcagcg aggagcgcga
      901 ccgcgccgag cgggcccatc gcgaggcgcg ccacgaccag ctcaccgggc tggccaaccg
      961 gcgccgcttc gacgagtacc tggccggcat gctgccgcgc gcgaccgacg agcagccgat
     1021 ctcgctcgtg ctgatcgatc tcgaccattt caagtcgatc aacgaccgct accgccacct
     1081 cgccggcgac gccgcgctgc gctgggtcgc gcgccacctg caggcgcagt gccgccaggt
     1141 cgacctgccg gcgcggctgg gcggcgacga gttcgccctg gtgctcaacg cgccgctggc
     1201 cgtcgcgcac caggtctgca cgcggctgcg ggtggcggtg gccgggcgct cgtccgagct
     1261 gccgtccgat gtcaccatcc gcttgagcgc cggcatagcg gaagcgaccg cgccgtgcga
     1321 tcctcagcac ctgttccgcc gcgccgacga ggcgctctac gcggccaagg ccggcggccg
     1381 cgacggcgtc cgccacgacg gcccgggcgc cggcgtccgc cggaccgaca aggaataccg
     1441 accatgacca ccgacctcca gcgcggcctc tacgacgccg accccgccat cgccgatgcc
     1501 gccccgccgg gcccgctgcc ggcgcaggag atcagcaccg aggtgctgat cgagaagtac
     1561 gccaagggcg acgaggacga cgtcgacgcg gtgcgccg
//
LOCUS       DASVLN010000804         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_257073, whole genome shotgun sequence.
ACCESSION   DASVLN010000804 DASVLN010000000
VERSION     DASVLN010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257073"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VF453_19095"
     CDS             <1..202
                     /locus_tag="VF453_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689821.1"
                     /translation="VPAAPAKPRDPFFDRPYEASAPEGAKPAWEKEAPATATPPVGRA
                     ALSANIRSKRKVAALLGGGSKN"
     gene            complement(283..1062)
                     /locus_tag="VF453_19100"
     CDS             complement(283..1062)
                     /locus_tag="VF453_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7689822.1"
                     /translation="MTARGRLLLMPNALDLGAAEVPLGDVLAAGVIAQAARLGHWIVE
                     DARSARAFLKRVDALAPLAQPLQQTAIAELPRPPKGGHRPPPAAGARGEASPWERLLA
                     PALAGHDVGLLSEAGLPGVADPGAFVVGAAHRLGIAVVALSGPSSITLALAASGLNGQ
                     SFVFHGYLPTDAGPRRARLRELEAASRKLDQTQVFIETPYRNAAMLEAAIDALAPTTR
                     LAIACGLTLAEGWASTRTAAEWKAARVALPDRVPAVFSLLA"
     gene            complement(1059..1679)
                     /locus_tag="VF453_19105"
     CDS             complement(1059..1679)
                     /locus_tag="VF453_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX7689823.1"
                     /translation="MAHDSSVPSSRPELILASTSRYRRELLERLRLPFRAVSPDVDET
                     PLPGEAPAALAERLALAKARAVSARFPDAVVIGSDQVADVDGRAIGKPGDHARATAQL
                     REMSGRAIVFQTAVAVVRASTGFAELRRAPVTTRFRTLTDAEIEFYLRAEQPYDCAGS
                     AKSETLGIALLEAIESDDPTALVGLPLMRTCALIRAAGLDPLGSAG"
BASE COUNT          245 a          717 c          763 g          229 t
ORIGIN      
        1 cgtccccgcg gcgccggcca agccgcgcga ccccttcttc gatcgccctt acgaggcctc
       61 cgccccggaa ggcgccaagc cggcgtggga gaaggaggcg ccggccaccg ccacgccgcc
      121 ggtcggccgc gcggcgctgt cggccaacat ccgctccaag cgcaaggtcg ccgcgctgct
      181 cgggggcggc tcgaagaact gacgcgcgtc gcgtctcccg cgcctggcca ggcccgtcgc
      241 acctccgtgc cgcgggcctt ttctttggcg gtcgccggcc gctcaggcga gcaggctgaa
      301 caccgccggc acgcggtcgg gcagggcgac gcgggcggcc ttccattcgg cggcggtgcg
      361 cgtcgaggcc cagccctcgg ccagcgtgag gccgcaggcg atcgccaggc gcgtcgtggg
      421 tgcgagcgcg tcgatcgcgg cctccagcat cgcggcgttg cggtagggcg tctcgatgaa
      481 gacctgcgtc tggtccagct tgcgcgaggc ggcctccagc tcgcgcaggc gggcgcgccg
      541 cgggcccgcg tccgtcggaa ggtagccgtg gaacacgaag ctctggccgt tcaggccgct
      601 ggccgccagc gcgagggtga tcgagctcgg cccgctcagc gcgacgacgg cgatgccgag
      661 ccggtgcgcc gcgccgacga cgaaggcgcc cgggtccgcg acgcccggca ggccggcctc
      721 gctcaggagc ccgacgtcgt ggccagcgag tgccggcgcg agcagccgct cccacggcga
      781 ggcctcgccg cgcgcgcccg cggcgggcgg cgggcgatgg ccccccttgg gcggccgcgg
      841 cagctccgcg atcgccgtct gctgcagcgg ctgcgcgagc ggcgcgagcg cgtcgacgcg
      901 cttgaggaag gcccgcgcgc tgcgcgcgtc ctcgacgatc cagtgcccga gccgcgccgc
      961 ctgcgcgatc actccggcag ccaggacgtc gcccagcggc acctcggccg cgccgagatc
     1021 cagtgcgttg ggcatcagca gcaggcggcc gcgggcagtc atccggcgct ccccagcggg
     1081 tcgaggccgg cggcgcggat cagcgcgcag gtgcgcatca gcggcagccc gacgagcgcg
     1141 gtcgggtcgt cggactcgat cgcctccagc agcgcgatgc ccagcgtctc ggacttggcg
     1201 ctgccggcgc agtcgtaggg ctgctcggcg cgcaggtaga actcgatctc cgcatcggtg
     1261 agcgtgcgaa agcgcgtcgt caccggcgcg cgccgcagct cggcgaagcc ggtggaggcg
     1321 cgcaccacgg ccaccgcggt ctggaacacg atcgcgcggc cgctcatctc gcgcagctgg
     1381 gcggtcgcgc gcgcgtggtc gccgggcttg ccgatggcgc ggccgtcgac gtcggcgacc
     1441 tggtccgagc cgatgaccac ggcatcgggg aagcgggcgg acaccgcgcg cgccttggcc
     1501 agcgcgaggc gttccgcgag agcggcgggg gcctcgccgg gcagcggcgt ctcgtcgacg
     1561 tcgggcgaga cggcgcggaa gggcaggcgc aggcgctcga gcagttcgcg gcggtagcgg
     1621 gaggtggagg cgaggatcag ctcggggcgg gaagacggta cggaggaatc gtgggccatg
     1681 gcggattgtc gcggggaacg atggcgtcgc catcgaagct cgcatcgaaa gatggcccgt
     1741 tgtcggatcg gcgtgcgcaa gcgcgtcggc ccgcgcggcc gttccttgcg ggccgcgccc
     1801 ataaaatcgg gcgatgacca cgcattcccc tgatccccgc cgtctcgacg tcgcccgctt
     1861 cgccgacgac gccggcgagc tcgacggcac ctggccggtc gccgagctga cccggttggc
     1921 cgacagcgaa tgcccgccgg acgccgcggg cgac
//
LOCUS       DASVLN010000805         7405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_95533, whole genome shotgun sequence.
ACCESSION   DASVLN010000805 DASVLN010000000
VERSION     DASVLN010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95533"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /gene="tolA"
                     /locus_tag="VF453_19110"
     CDS             <1..661
                     /gene="tolA"
                     /locus_tag="VF453_19110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX7689824.1"
                     /translation="PPPPPPAPAPAPAPEPAPAPPPPVVAPKAPDIVTEQLKEKQRQQ
                     QLREQQEKLEQQKLAQQKAEQAKAEQAKAEAQKKAEQQKQAQQQKELDARIIAKLREQ
                     NLAAMRAQLSGAGVPMQSGTAAQSRGPSADYAGRIKARVRPNIVFDNSTIPGNPEADV
                     EVRCAPDGTIIGRRIVKSSGVAAYDDAVLRALDRTQVLPRDKDGSIPNPMTIAFHPQD
                     Q"
     gene            complement(704..970)
                     /locus_tag="VF453_19115"
     CDS             complement(704..970)
                     /locus_tag="VF453_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEX7689825.1"
                     /translation="MSHRPLATSALASLLALGLAQAAHAADDNKNKEQCYGIAKAGQN
                     DCASPTGTHSCAGQAKKDNSPDDWKYVAKGTCKSLGGATQPPKK"
     gene            1231..3450
                     /locus_tag="VF453_19120"
     CDS             1231..3450
                     /locus_tag="VF453_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7689826.1"
                     /translation="MPDPHPDVPSAAIADSLFTDTLQGLSIGVLWTTVDARLRHANKI
                     ASAWLGRGEGGLQGASLFDLVEDFGLADWSRLLHDSAGTQGLHRLTIAPPGGARRTLG
                     VRPAALVHDGQPMVALLMAPIEDRLEAESIEGLQREVLEAIALGRPLNAVLDLLCRRV
                     ESLAPGLACSVLLIEKNGTVTPAAGPSLPPAYVEALAGAPIGPRAGSCGTAAWRREAV
                     EVTDIATDPLWADFLGLLPLLGMAACWSTPIIATSGEVVATFALYYRDKGSAPPYHRR
                     MVEACVPLCRVAIQHEANRLEIERLAYFDPLTGLPNRRLFADRARQALQLAAHSHGAG
                     ALLLLDLDRFKTTNDSLGHGAGDEVLRETARRLQREVGESATVARLGGDEFVALLPRC
                     GPVEAMHVAERLRLALAAPLRVAGMQIEVSTSVGISVFPQDGSDLDRLLKNAEMAMYE
                     AKRAGRGTARFFTSGMNDVIAQRLQMESALRQAVAARALELHYQPKIALADGACTGVE
                     ALVRWTDPVLGAVPPDRFIALAEECGLVGAIDAWVLETACARLARWQRAGVAVPAVSV
                     NVSPLRLHQDDVPAHVRRVLADNGLPPAALSLEITERVMLSDDERVRADLHALHDMGV
                     RLSVDDFGTGYSSLGYLKRLPVAELKIDRSFVADLERDESDRALASAVIGIGRALGLQ
                     VVAEGVETAGQRAVLEDIGCDAVQGFLYTRALPADGLEAWLAARDAAATAGPQVANG"
     gene            3522..4097
                     /locus_tag="VF453_19125"
     CDS             3522..4097
                     /locus_tag="VF453_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7689827.1"
                     /translation="MPDDFARQLEELQPQLLRFARTQLRNDAWAEDAVSETVVAALEK
                     PQAFSGRSQLKTWLVGILKHKVIDQLRRHKREATVLTSDDEEDLDELLFAADGHWREV
                     PADWGNPDEVLRQRQFIDVMEACVEALPPTQARVFMMREWLELSSDEVCKELTITSTN
                     LWVLLHRARLRLRECLQAGWFDAHPATAARP"
     gene            4094..4321
                     /locus_tag="VF453_19130"
     CDS             4094..4321
                     /locus_tag="VF453_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7689828.1"
                     /translation="MSFINRNCREVTRLILEREHRPLTTVERLAVRLHLGICLMCTRF
                     NGQLAVMNRALDAWKSYREQDADEQAGPATE"
     gene            4455..7352
                     /locus_tag="VF453_19135"
     CDS             4455..7352
                     /locus_tag="VF453_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7689829.1"
                     /translation="MSNLSQPFHTVQSAQSSRWAMPPGANAGASWPLSGAEPCSILGL
                     NDKSFNARLAQFDADAGTATVYVPPARSPMQLRFSQFQRLTLTDPLTPSTNGVNAAPG
                     WSAGAPGDTEYQVLLSNGKSASGRTIGHVEDERGLFLFPPVGDAGLVQRVFYPRDAYQ
                     RVNFGQRLGDVLVSQHTLSPQQLQEALAEQQRIRNLKLGEMLVEVQLITQPQLNQALE
                     LQARTPLLRLGEALISLEILSAEDLARALRSQARNRSLPLGELLVNHGVITRDELRDA
                     LEVKRASSPGAAEADTVFGDLPELELLDVPPPAAAHAPADKPLPAIDPAPPVRAREQR
                     VSDALLSAHVTDPQQLAAALDAQSRMPMVRIGEALVALGLITQQQLTEALASQGKDRK
                     QPLGEMLVRQGLITRIDLATALARKMGYPQVDLAAFPIEIEALRKLPVSAAQRLEMMP
                     LAMLGGRLVVAMDDPSRRDRLDEAEFLAQNKIVPVLPASANLLEQIAPLYARGGLDEM
                     TLDFNMPGANGEPQGTDTLLAALEIPAESALAVDDDDRAVEQSDSSLVRLINTMIVEA
                     HSMGASDIHIETRPGKEKVRVRFRVDGVLRPHLELPHTYRSALVARLKIMCDLDISER
                     RRPQDGKINFARFVPSCPIELRVAMIPTNNGLEDAVLRILASSKPIPLDNLGMSAHNL
                     AALKAAIERPYGMVLCVGPTGSGKTTTLHSALNFINTPERKIWTAEDPVEITQAGLRQ
                     VQVNPKIDWTFAKALRAFLRADPDVIMVGEIRDQETAEIAIESSLTGHLVLSTLHTNS
                     APETITRLLDMGMDPFNFADALLAVLAQRLVRRICKDCRTSRPASQDEVDELLNDALH
                     VWGDHPRRPTREDLLADWHERHAVDGRLMHWHGAGCAKCGNTGLKGRAGIHELLTVTR
                     AVRTLIQKGARAEEILHAAMGEGLRTLRQDGIEKVLAGVTTIEEVRATSNA"
BASE COUNT         1065 a         2786 c         2592 g          962 t
ORIGIN      
        1 tccgccgccg cctccgcccg cgccggcacc cgcccctgcg cccgaacccg ctcccgcgcc
       61 gccgccaccc gtcgtcgcgc ccaaggcgcc ggacatcgtc accgagcagc tcaaggaaaa
      121 gcagaggcag cagcagctgc gcgagcagca ggagaagctc gagcagcaga agctggcgca
      181 gcagaaggcg gagcaggcca aggccgagca ggcgaaggcg gaggcacaga agaaggccga
      241 gcagcagaag caggcgcaac agcagaagga gctcgacgcc aggatcatcg ccaagctgcg
      301 cgagcagaac ctggcggcga tgagggcgca gctctcgggc gccggcgtgc cgatgcagtc
      361 cgggacggcg gcgcagagcc gcggcccgtc ggccgactac gccgggcgca tcaaggcgcg
      421 cgtgcggccc aacatcgtgt tcgacaacag cacgatcccg ggcaaccccg aggccgacgt
      481 cgaagtgcgc tgtgcgcccg acggcacgat catcggacgc cgcatcgtca agtccagcgg
      541 cgtggcggcc tacgatgacg ccgtgctgcg cgcgctggac cgcacgcagg tgctgccgcg
      601 cgacaaggat ggctcgatcc cgaacccgat gacgatcgcc ttccacccgc aggaccagtg
      661 atccgcgggc gccgcgctgc ccgcggtcgg gtcgcggccc ggcctacttc ttcggcggct
      721 gcgtcgcccc gcccagcgac ttgcaggtgc ccttggcgac gtacttccag tcgtcgggcg
      781 aattgtcctt cttggcctgg ccggcgcacg agtgcgtgcc ggtcgggctc gcgcagtcgt
      841 tctggccggc cttggcgatg ccgtagcact gctccttgtt cttgttgtcg tcggcggcgt
      901 gggcggcctg tgcgagcccg agggcgagca gcgacgcgag ggccgaggtg gcgaggggac
      961 ggtggctcat ggcggattcc cggctggaga tggacgaccc ggctggccgg gcggaggtgc
     1021 gcgcagtgtc gccgaagcct gcccgggcgc caaccggccc gcggctgcag cgcgacgaat
     1081 gccacgattg cacgccaatc gtgaactccc gacacgcgac tcccgcgatt gctcgttgcg
     1141 gtcgcggggg acgccgctca caatggcccg atcccgcctc ccggcgcggc cgcccgtcgc
     1201 cgcgcccgac ccggacaccg gagtccgcgc ttgcccgacc cccatcccga cgtcccgtcc
     1261 gccgcgatcg ccgacagcct gttcaccgac acgctccagg gcctgtccat cggcgtgctg
     1321 tggacgaccg tcgatgcgcg cctgcgccac gccaataaga tcgccagcgc ctggctgggc
     1381 cgcggcgagg gcgggctgca gggcgcgtcg ctgttcgacc tcgtggagga tttcgggctc
     1441 gccgactgga gccgcctcct gcacgactcg gccggcacgc agggcctgca ccgcctgacc
     1501 atcgcgccgc ccggcggcgc gcggcggacg ctcggcgtgc gtcctgcggc gctggtgcac
     1561 gacggccagc cgatggtcgc gctgctgatg gccccgatcg aggaccggct cgaggccgag
     1621 agcatcgagg gcctgcagcg cgaggtgctc gaggcgatcg ccctcggccg gccgctgaac
     1681 gcggtgctcg acctcttgtg ccggcgcgtc gagtcgctcg cgcccggcct cgcgtgctcc
     1741 gtgctgctga tcgagaagaa cggcacggtg acgcccgcgg ccggccccag cctgccgccg
     1801 gcctacgtcg aggcgctggc cggcgcgccg atcggcccgc gcgccggctc gtgcggcacg
     1861 gcggcgtggc gacgcgaggc ggtcgaggtc acggacatcg cgaccgaccc gctgtgggcc
     1921 gacttcctcg gcctgctgcc gctgctgggc atggccgcgt gctggtcgac gcccatcatc
     1981 gccacgagcg gcgaggtggt ggcgaccttc gcgctgtact accgcgacaa gggctcggcg
     2041 cccccctatc accgccgcat ggtcgaggcc tgcgtgccgc tgtgccgggt ggcgatccag
     2101 cacgaggcca accggctcga gatcgagcgc ctggcctact tcgacccgct caccggcctg
     2161 cccaaccggc gcctgttcgc cgaccgggcg cgccaggcgc tgcagctcgc cgcgcacagc
     2221 cacggcgccg gggcgctgct gctgctggac ctcgaccgct tcaagaccac caacgactcg
     2281 ctcggccacg gcgccggcga cgaggtgctg cgagagacgg cgcgtcggct ccagcgcgag
     2341 gtcggcgagt cggccacggt cgcgcgcctg ggcggcgacg agttcgtggc cctgctgccg
     2401 cgctgcggcc cggtcgaggc gatgcacgtg gccgagcggc tgcggctggc gctggcggcg
     2461 ccgctgcggg tggcgggcat gcagatcgag gtgtcgacca gcgtcggcat cagcgtcttc
     2521 ccgcaggacg gctccgacct cgaccgcctg ctgaagaacg ccgagatggc catgtacgag
     2581 gccaagcgcg ccggccgcgg caccgcgcgc ttcttcacga gcgggatgaa cgacgtgatc
     2641 gcgcagcgcc tgcagatgga gagcgcgctg cgccaggcgg tcgccgcgcg cgcgctggag
     2701 ctgcactacc agcccaagat cgccctggcc gacggcgcct gcaccggcgt cgaggcgctc
     2761 gtgcgctgga cggatcccgt gctgggcgcc gtgccgcccg accgtttcat cgcgctcgcc
     2821 gaggagtgcg ggctggtcgg cgcgatcgac gcctgggtgc tcgagaccgc gtgcgcccgg
     2881 ctcgcccgct ggcagcgcgc cggcgtggcc gtgccggcgg tctccgtcaa cgtctcgccg
     2941 ctgcgcctgc accaggacga cgtcccggcc catgtccgcc gcgtgctggc cgacaacggc
     3001 ctgcctccgg ctgcgctctc gctggagatc accgagcgcg tcatgctcag cgacgacgaa
     3061 cgcgtgcgcg ccgacctcca cgcgctgcac gacatgggcg tgcggctgtc ggtggacgac
     3121 ttcggcaccg gctactccag cctcggctac ctcaagcggc tgccggtcgc ggagctcaag
     3181 atcgaccgca gcttcgtggc cgacctcgaa cgcgacgaga gcgaccgcgc gctcgcctcg
     3241 gcggtcatcg gcatcggccg ggcgctcggg ctgcaggtcg tcgccgaggg cgtcgagacc
     3301 gccggccagc gcgccgtgct cgaggacatc ggctgcgatg ccgtgcaggg cttcctgtac
     3361 acgcgggcgc tgcctgccga cggcctcgag gcctggctcg cggcccgcga cgcggccgcg
     3421 acagccggcc cgcaggtggc gaacggctga ggccgcggcg ccgttcagcg atactcccgc
     3481 tgcgccggcc cgacggcgcg ccccgctgca acgccccgcc catgcccgac gacttcgccc
     3541 gccagctcga ggagctccag ccccagttgc tgcgcttcgc gcgcacccag ctgcgcaacg
     3601 acgcgtgggc cgaggacgcc gtctcggaga ccgtggtcgc cgcactcgag aagccccagg
     3661 ccttctccgg ccgttcgcag ctgaagacct ggctggtggg catcctcaag cacaaggtga
     3721 tcgaccagct gcgccgccac aagcgcgagg ccacggtgct cacgtcggac gacgaggagg
     3781 acctcgacga gctgctgttc gccgccgacg gccactggcg cgaggtgccg gccgactggg
     3841 gcaacccgga cgaggtgctg cgccagcgcc agttcatcga cgtgatggag gcctgcgtcg
     3901 aggcgctgcc tccgacccag gcgcgcgtgt tcatgatgcg cgagtggctg gagctgtcct
     3961 ccgacgaggt ctgcaaggaa ttgaccatca cgtcgaccaa cctgtgggtg ctgctgcatc
     4021 gcgcccgcct gcgcctgcgc gaatgcctgc aggccggctg gttcgacgcc catcccgcca
     4081 ctgccgcccg cccatgagct tcatcaaccg caactgccgc gaggtcacgc gcctgatcct
     4141 cgagcgcgag caccggccgc tcacgacggt ggagcgcctg gccgtgcggc tgcacctggg
     4201 catctgcctg atgtgcaccc gcttcaacgg gcaactggca gtgatgaacc gggccctgga
     4261 cgcgtggaag tcctaccgcg agcaggacgc ggacgagcag gccggccccg cgaccgagtg
     4321 agcgccgacg cgcggcaggc gcggatctgg ggctcgatcg cgcgcctttt ctccgcttcg
     4381 cggcggcgcg ccgtccctac catcgaatcg accccgcctg tcgccggggg ttgccgcgtg
     4441 cccacgcccg aacgatgtcc aacctgtccc agccgttcca caccgtccag tccgcgcagt
     4501 cctcgcgctg ggccatgccg ccgggcgcca acgccggcgc gtcgtggccg ctgagcggtg
     4561 cggagccctg cagcatcctc ggcctgaacg acaagagctt caatgcgcgc ctggcccagt
     4621 tcgacgccga cgcgggcacc gccaccgtct acgtgccgcc cgcgcgctcg ccgatgcagc
     4681 tgcgcttctc gcagttccag cggctgaccc tcaccgatcc gctgaccccc tcgaccaacg
     4741 gcgtcaacgc tgcgcccggc tggagcgccg gcgcgcccgg cgacaccgag taccaggtgc
     4801 tgctgagcaa cggcaagtcc gcgagcggcc gcaccatcgg ccacgtcgag gacgagcgcg
     4861 gcctgttcct gttcccgccc gtgggtgacg ccggcctggt gcagcgcgtc ttctacccgc
     4921 gcgacgccta ccagcgcgtc aacttcggcc agcgcctggg cgacgtgctc gtcagccagc
     4981 acacgctgtc gccgcagcag ctgcaggaag cgctcgccga gcagcagcgc atccgcaacc
     5041 tcaagctcgg cgagatgctc gtcgaggtgc agctgatcac gcagccgcag ctgaaccagg
     5101 cgctcgagct gcaggcgcgc acgccgctgc tgcggctggg cgaggcgctg atctcgctgg
     5161 agatcctgtc ggccgaggat ctcgcccgcg ccctgcgcag ccaggcacgc aaccgcagcc
     5221 tgccgctggg cgagctgctg gtcaaccacg gcgtcatcac gcgcgacgag ctgcgcgacg
     5281 cgctcgaggt caagcgcgcg agctcgcccg gtgcggccga ggccgacacc gtcttcggcg
     5341 acctgcccga gctcgagctg ctggacgtcc ccccgccggc ggccgcccat gcgccggccg
     5401 acaagccgct gccggcgatc gatccggcgc cgcccgtgcg cgcgcgggag caacgcgtga
     5461 gcgacgcgct cctcagcgcc cacgtcaccg acccgcagca gctcgccgcg gcgctggacg
     5521 cgcagagccg gatgcccatg gtgcgcatcg gcgaggcgct ggtcgcgctc ggcctcatca
     5581 cgcagcagca gctcaccgag gcgctcgcca gccagggcaa ggaccgcaag cagccgctcg
     5641 gcgagatgct ggtgcgccag gggctgatca cgcgcatcga cctcgcgacc gcgcttgccc
     5701 gcaagatggg ctatccgcag gtcgacctcg cggccttccc gatcgagatc gaggcgctgc
     5761 gcaagctgcc cgtgtccgcg gcgcagcggc tcgaaatgat gccgctggcc atgctgggcg
     5821 gccggctcgt ggtcgcgatg gacgatccgt cgcgccggga ccgcctcgac gaggccgagt
     5881 tcctcgccca gaacaagatc gtcccggtgc tgccggcgtc ggcgaacctc ctcgagcaga
     5941 tcgcgccgct gtatgcgcgc ggcggcctcg acgagatgac gctggacttc aacatgcccg
     6001 gcgcgaacgg cgagccgcag ggcaccgaca cgctgctggc ggcgctcgag ataccggccg
     6061 agtctgcgct ggccgtcgac gacgacgacc gcgccgtgga gcagtccgac agctcgctcg
     6121 tgcgcctgat caacaccatg atcgtcgagg cgcactcgat gggcgcctcg gacatccaca
     6181 tcgagacgcg ccccggcaag gagaaggtgc gcgtgcgctt ccgcgtcgac ggcgtgctgc
     6241 gcccgcacct ggagctgccg cacacctacc gctccgccct cgtcgcgcgc ctgaagatca
     6301 tgtgcgacct cgacatctcc gagcgccgcc gcccccagga cggcaagatc aacttcgccc
     6361 gcttcgtgcc ctcgtgcccg atcgagctgc gcgtggcgat gatcccgacc aacaacggcc
     6421 tcgaggacgc cgtgctgcgg atcctggcct cgtccaagcc gatcccgctc gacaacctgg
     6481 gcatgtccgc gcacaacctc gccgcgctga aggcggccat cgagcgcccc tacggcatgg
     6541 tgctgtgcgt cggcccgacg ggctccggca agacgacgac gctgcactcg gcgctgaact
     6601 tcatcaacac gcccgagcgc aagatctgga cggccgagga tccggtggaa atcacccagg
     6661 ccggcctgcg ccaggtgcag gtcaacccga agatcgactg gaccttcgcc aaggcgctgc
     6721 gcgcgttcct gcgcgcggat ccggacgtga tcatggtcgg cgaaatccgc gaccaggaga
     6781 ccgccgagat cgcgatcgag tcctcgctga ccggccacct ggtgctgtcc acgctgcaca
     6841 cgaacagcgc gcccgagacc atcacgcgcc tgctggacat gggcatggat ccgttcaact
     6901 tcgccgacgc gctgctggcc gtgctggcgc agcgcctggt gcgccgcatc tgcaaggact
     6961 gccgcacctc gcgccccgcg agccaggacg aggtcgacga gctgctgaac gacgccctgc
     7021 acgtgtgggg cgaccatccg cgccgcccga cgcgcgagga cctgctcgcc gactggcacg
     7081 agcgccacgc cgtcgacggc cgcctgatgc actggcacgg cgcgggctgc gccaaatgcg
     7141 gcaacacggg cctcaagggc cgcgccggca tccacgagct gctgacggtg acgcgcgccg
     7201 tgcgcaccct gatccagaag ggcgcgcgcg ccgaggagat cctccacgcc gccatgggcg
     7261 aaggcctgcg cacgctgcgc caggacggca tcgagaaggt gctcgcgggc gtgacgacga
     7321 tcgaggaagt gcgggcgacg agcaacgcgt agcggcggcg ccctcggtgc ggccggtgtt
     7381 cgacgccgcg ccctgccgcg atgcg
//
LOCUS       DASVLN010000806         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_527053, whole genome shotgun sequence.
ACCESSION   DASVLN010000806 DASVLN010000000
VERSION     DASVLN010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527053"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..397)
                     /locus_tag="VF453_19140"
     CDS             complement(11..397)
                     /locus_tag="VF453_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7689830.1"
                     /translation="MADTSNDIALSQSDRVAQQRSIAPAVDVLEDPHGITLLADMPGV
                     ARERLEVRLDGDTLAVEGAVDLDAPADMRALWAEVNVPRYRRTFRLSRELDPSRIEAT
                     LKDGVLSLRIPRQPQAQPRRIAVEAG"
     gene            complement(445..891)
                     /locus_tag="VF453_19145"
     CDS             complement(445..891)
                     /locus_tag="VF453_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7689831.1"
                     /translation="MLDTLFHRSQGLFDQLEALHRTLSGTLGPNGAPDAIRSVAAGSF
                     PAINVGRTAQSVEVHAYAPGLDPAAIDVTVERGVLRISGERRPPAFEGGVQAYAAERP
                     HGRFLRAVTLPDDIDTTSVQARYRDGVLRVSIGLQQSAQPQRIAVQ"
     gene            complement(1042..1242)
                     /locus_tag="VF453_19150"
     CDS             complement(1042..1242)
                     /locus_tag="VF453_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689832.1"
                     /translation="MAQPFAPTGQAVAGRPELPAAAARADSALHAYLLARETLRRVKP
                     TATPCFAVDADERRRRQQPRHG"
BASE COUNT          186 a          552 c          678 g          235 t
ORIGIN      
        1 cgccggcgcg tcagccggcc tcgaccgcga tgcggcgcgg ctgcgcctgc ggctggcgcg
       61 ggatgcgcag gctcaggacg ccgtccttca gcgtggcctc gatgcgcgac gggtcgagct
      121 cgcggctcag gcggaacgtg cggcgatagc gcggcacgtt gacctcggcc cacagcgcgc
      181 gcatgtcggc gggcgcgtcg aggtccacgg cgccctcgac ggcgagcgtg tcgccgtcca
      241 ggcgcacctc caggcgttcg cgcgcgacgc cgggcatgtc ggcgagcagg gtgatgccgt
      301 gcggatcctc gagcacgtcg accgcgggcg cgatgctgcg ctgctgggcg acgcggtcgg
      361 attgcgagag ggcgatgtcg ttgctggtgt ctgccatggt cgtttcctcc ggaagacaag
      421 gtggcgttgg ggccgcgcga gcgatcactg cacggcgatg cgttgcggct gcgcggactg
      481 ctgcaggccg atgctcacgc gcagcacgcc gtcgcggtag cgcgcctgca cgctggtggt
      541 gtcgatgtcg tcgggcagcg tgaccgcgcg caggaagcgg ccgtgcgggc gttccgcggc
      601 gtaggcctgg acgccgccct cgaacgcggg cggacggcgc tcgcccgaga tgcgcagcac
      661 gccgcgctcg accgtcacgt cgatggcggc ggggtcgagg cccggcgcgt aggcgtgcac
      721 ctcgaccgac tgggcggtgc ggccgacgtt gatcgccggg aagctgccgg ccgccaccga
      781 gcggatggcg tccggcgcgc cattgggccc gagcgtcccg gacagcgtcc ggtgcagcgc
      841 ctcgagctgg tcgaagagcc cttgcgagcg gtggaagagg gtgtcgagca tggtggactc
      901 ctgggtcggg ttcgtggcga cgacgccgcg ctcgtcgtcg gccgctgtcg cgggcggagc
      961 gcaggcgcgc gccggcaagg ctccgcccgt cggcgcatcg ctgcaccgcg cggggtgtcg
     1021 gtcgtgggtg gaaggcctgg gctagccgtg gcgcggctgc tgccttcggc ggcgctcgtc
     1081 ggcgtccacc gcgaagcagg gcgtggcggt cggcttgacc cggcgcagcg tctcgcgtgc
     1141 gagcaggtac gcgtgcagcg cactgtccgc ccgcgcggcg gctgccggaa gttcggggcg
     1201 gccggcgacg gcctgcccgg tgggagcgaa tggctgagcc atgattcagt tcctcatccg
     1261 aatcgggtgt tggctgggtc gattccggct ccgaattagg gtcgcgcgcg tggcgcatca
     1321 agggcctcgt gattcaggga aaaccctgat ccgaccgctt cggagggaac agggtcgcgg
     1381 gtgcggccgt cggcaggcgc ccggctggca gctccggcgc tcccgccact cggggacggg
     1441 cgttccaggc cggctcgccc ggccgggccc agcgggcgcg cgggggcggt cctcctcggg
     1501 ttggcactcg ccgcggagcc gggctgcagg cgccagtcgc gagcgtcggc cggcgtgtcg
     1561 atctcgtcgc cgcagcgcat cgccgctgac ggcgggatcg ggtcgctcgc ggggacgtga
     1621 ggcgagcgac tcgtcgagca acgccgggta g
//
LOCUS       DASVLN010000807         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_152374, whole genome shotgun sequence.
ACCESSION   DASVLN010000807 DASVLN010000000
VERSION     DASVLN010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152374"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VF453_19155"
     CDS             <1..471
                     /locus_tag="VF453_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7689833.1"
                     /translation="LALGIAASIASSAGSIVVGKAREESNNLVLTTGWSMFWGTCMVA
                     AWCLATGQAFIIPRTASYVLGLLHLSIFGSVVAFICYFTLINRIGSNKAVYIGVITPV
                     ISVLLSIKLEHYRPGLTEWLGMAVCLGSVAWALRSPAARPAASVNLNDCIETTP"
     gene            468..959
                     /locus_tag="VF453_19160"
     CDS             468..959
                     /locus_tag="VF453_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7689834.1"
                     /translation="MTPATTFAIRPATPADVTHIQSMIVELAVFEKLEHLVVATEEKL
                     HEGLFGPHPACEAIVGEADGEVVTFALFFHNFSTFLTKRGLYLEDLYVRQSHRGKGYG
                     SRMLKHLARLAVERGCGRFEWSVLDWNTPAINFYQSMGAEVMPDWRICRVTGAPLEAL
                     AQG"
     gene            1205..1372
                     /locus_tag="VF453_19165"
     CDS             1205..1372
                     /locus_tag="VF453_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689835.1"
                     /translation="MKMITRWVYRVWLRSRIDTYTRSLHAIAVQRENDFHAERILHRA
                     ISTTRSKLQSL"
     gene            complement(1360..3024)
                     /locus_tag="VF453_19170"
     CDS             complement(1360..3024)
                     /locus_tag="VF453_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7689836.1"
                     /translation="MILAPAFAFVLARLRGSGRLVLALALAASAARAADFVNDVPRAL
                     ARAPAAPASTARTGGDAFVVGSKRFTESYILGEILRQAAATAGPAEHRQGLGNTAVVL
                     AALKAGAIDAYPEYLGTIDAEILGHRTPSPPARIERELAAQGLGMGVPLGFSNGYALA
                     TTAELARRLHLSRVSDLAAHPGLVLGLSHEFLGRADGWPGLSQRYGLAQKPIGIDHGL
                     AYDALASGRIAVTDIYTTDAQIAARHLVVLQDDRGYFPRYDAVLLYRLDAVARHPAQW
                     RAISALQGRIGVDAMIAMNSRAERLHEPFADIARDFAAGRIDPRREADAAAASLKPSS
                     RAGTAPGIAPAGLLARLFADDLPRLLGEHLLLVGVAVLCATLLGVPLGALAAARRRSE
                     GPIMAAVGLLQTVPSLALLAILIPLLGRIGTVPALVALCAYALLPIVRNTCTGLLQVP
                     SGLRTAALALGLTRRQSLVEVELPLAMPTILAGVKTAAVMSVGTATIAAFIGAGGFGE
                     RITTGLALNDNDMLLAGAIPAALLALLTQAGFEVCERLLARRRYSD"
     gene            3130..>3584
                     /locus_tag="VF453_19175"
     CDS             3130..>3584
                     /locus_tag="VF453_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689837.1"
                     /translation="MSDPTVPDEGAALHAGTDALASLAQRERFLDALRAALADGSVSR
                     VLLSKPVDKGDDVERVSARPLALRGEPHLSFTWKHRTRDVTKNEPVATGVETIASLLG
                     GRFAHAHLFGRDAELQLMMSRKGRWTLHRTRAGQPARADATGPAVDAARA"
BASE COUNT          574 a         1248 c         1249 g          513 t
ORIGIN      
        1 ctcgcgttgg gcatcgccgc gtcgatcgcc agctcggccg gcagcatcgt cgtcggcaag
       61 gcgcgcgagg aatcgaacaa cctggtgctg acgacgggct ggtcgatgtt ctggggcacc
      121 tgcatggtcg ccgcgtggtg cctcgcgacg ggccaggcat tcatcatccc gcgcacggcc
      181 agctacgtgc tgggcctgct ccacctgtcc atcttcggct cggtcgtcgc gttcatctgc
      241 tactttacgc tgatcaaccg catcggctcg aacaaggccg tgtacatcgg cgtgatcacg
      301 cccgtgatct ccgtgctgct gtcgatcaag ctcgaacact accgccccgg cctcaccgaa
      361 tggctgggca tggccgtgtg cctgggcagt gtcgcctggg cattacgttc ccctgcggcc
      421 aggccggccg cttccgtcaa cctcaacgat tgcatcgaaa cgactccatg actcctgcta
      481 ccaccttcgc gatccgcccc gccacgccgg ccgacgtcac ccacatccaa tcgatgatcg
      541 tcgagctggc cgtgttcgaa aagctcgagc acctcgtcgt ggcgacggaa gagaaattgc
      601 acgaaggcct gttcggcccg catccggcgt gcgaagccat cgtcggcgaa gcggatggcg
      661 aagtcgtcac gttcgcgctc ttcttccaca atttctcgac gttcctcacc aagcgtgggc
      721 tgtatctgga agatctgtac gtgaggcaat cgcatcgcgg caaaggttat ggcagccgca
      781 tgctgaagca cctggcacgc ctggccgtcg agcgcggctg cggccgcttt gaatggtcgg
      841 tgctggactg gaacacgccg gcaatcaatt tctaccagtc gatgggcgca gaggtcatgc
      901 cggactggcg catctgccgc gtcacgggcg cgccgctgga agcgctggcc caaggctgat
      961 tcaggcaaga aaaaacccgc gggctgtggc gcagcgccgc gggtgaaccc attcgtcgat
     1021 ggggagggga gacttgaatc caacggcttc aatatagtct ccccaccgcc gcatttgcag
     1081 attccttaca aatgcttacc tcggtaacag aaacctggat ttttgtgggc cggccccgat
     1141 tgagtgatca caaatgcaag aacgcgtcga gcgggacaat gtggtcattc acgaggacat
     1201 ggacatgaaa atgatcaccc gatgggtcta ccgggtttgg ctccgctccc ggatcgacac
     1261 ctatacccgc agcctgcacg ccatcgccgt ccagcgcgaa aacgacttcc acgcggaacg
     1321 catcctgcac cgcgcgatct ccacgacccg ttccaaactt cagtcgctgt agcgccgccg
     1381 ggccagcaac cgctcgcaca cctcgaaccc cgcctgggtc agcaaggcca gcagcgcggc
     1441 cgggatcgcg cccgccagca gcatgtcgtt gtcgttcagc gcgagacccg tcgtgatgcg
     1501 ttcgccgaag ccgccggcgc cgatgaaggc ggcgatggtt gccgtgccga cgctcatcac
     1561 cgccgccgtt ttcacgccgg cgaggatcgt cggcatggcc agcggcagct cgacctcgac
     1621 cagcgattgc cgccgtgtca gccccagcgc cagcgccgcc gtgcgcaggc cggacggcac
     1681 ctgcagcagc cccgtgcacg tattgcgcac gatgggcagc agcgcatacg cgcacagggc
     1741 gacgagcgcg ggaacggtgc cgatgcggcc gagcaacggg atcaggatcg cgagcagcgc
     1801 gagcgacggc acggtctgca gcagcccgac cgcggccatg atcgggccct cgctgcgccg
     1861 ccgtgccgcg gccagcgcgc ccagcggcac gcccagcagc gtcgcgcaca gcaccgccac
     1921 gccgacgagc agcaggtgct cgccgagcag ccgcggcagg tcgtcggcga acaggcgcgc
     1981 caggaggccc gcaggcgcga tacccggtgc cgtaccggcg cgcgacgacg gcttcagcga
     2041 cgcggccgcg gcgtcggcct cgcgcctcgg atcgatgcgc ccggcggcga agtcacgcgc
     2101 gatgtcggcg aagggctcgt gcaggcgttc ggcgcggctg ttcatcgcga tcatcgcatc
     2161 gacgccgatg cgtccctgaa gtgccgagat cgcccgccac tgcgccggat ggcgcgcgac
     2221 ggcgtcgagg cggtacagca ggacggcgtc gtagcgcggg aagtagccgc gatcgtcctg
     2281 cagcacgacg aggtggcgcg ccgcgatctg ggcgtccgtc gtgtagatgt cggtgacggc
     2341 gatgcggccg ctcgcgagcg cgtcgtaggc caggccgtgg tcgatgccga tgggcttctg
     2401 cgcgaggccg tagcgctgcg agagacccgg ccagccgtcg gctcggccga ggaactcgtg
     2461 cgacaggccc agcaccagtc ccggatgcgc cgcgaggtcg ctgacgcgcg acaggtgcag
     2521 gcgccgcgcg agctcggcgg tcgtcgcgag cgcgtagccg ttcgagaagc ccagcggcac
     2581 gcccatcccg agcccctgcg cggcgagctc gcgctcgatc cgggccggcg ggctcggcgt
     2641 ccggtggccg aggatctcgg cgtcgatggt gcccaggtac tcggggtagg catcgatcgc
     2701 gccggccttg agcgccgcca gcacgacggc cgtgttgccc aggccctggc ggtgctcggc
     2761 cgggccggcg gtcgcggcgg cctggcgcag gatctcgccg aggatgtacg actcggtgaa
     2821 gcgcttggag ccgacgacga aggcgtcgcc gccggtacgc gcggtcgagg ccggcgccgc
     2881 gggcgcgcgg gcgagcgcgc gcgggacgtc gttcacgaag tcggcggccc gggcggcgga
     2941 agcggccagc gcgagcgcca ggacgagccg gccgctgccg cgcagccggg cgaggacgaa
     3001 ggcgaaggcc ggggccagga tcatgcggaa gctcgcgcgt gcggatgggg acggcaatcc
     3061 tcgcacgaca ggcgcgtcga cgggcgcctg cgcgaaaatg ccggcgtgcc cttccctgac
     3121 cgacccctcg tgtccgatcc caccgttcct gacgaaggcg ccgcgctgca tgccggcacc
     3181 gacgcgctcg cgagcctcgc gcagcgcgaa cgcttcctcg acgcgctgcg cgccgcgctc
     3241 gccgacggca gcgtgagccg cgtgctgctc agcaagccgg tcgacaaggg cgacgatgtc
     3301 gagcgcgtga gcgcgcggcc gctggccctg cgcggcgagc cgcacctgag cttcacctgg
     3361 aagcaccgca cccgggatgt gaccaagaac gagccggtcg ccacgggcgt ggagaccatc
     3421 gcgagcctgc tgggcgggcg cttcgcgcat gcgcacctgt tcggtcggga cgcggagctc
     3481 cagttgatga tgagccgcaa gggtcgctgg acgctgcacc gcacgcgcgc cgggcagcct
     3541 gcgcgcgccg acgcgaccgg ccccgcggtc gacgcggcgc gcgc
//
LOCUS       DASVLN010000808         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_417284, whole genome shotgun sequence.
ACCESSION   DASVLN010000808 DASVLN010000000
VERSION     DASVLN010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417284"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VF453_19180"
     CDS             complement(<1..365)
                     /locus_tag="VF453_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7689838.1"
                     /translation="MSRPTFDLDVLRTLVTGIELGSFAKAADRLGRSTSAVSAQLKKL
                     EEAAGAPVLRKAGRGMVLTPTGEVLFGYARRLLALDDEAAHAVRGAELHGAVRLGLQE
                     DFGEGLLTGVLAGFARAHPR"
     gene            468..788
                     /locus_tag="VF453_19185"
     CDS             468..788
                     /locus_tag="VF453_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7689839.1"
                     /translation="MTQPSTVAAQMYGDVAPKFTELTDQLLFDDIWKRPQLSPRDRSL
                     ATVSALIALYRLEQLPFHLDRALDNGVTREELAELVAHLAFYAGWPCAASATKLLAAA
                     LARR"
     gene            803..1201
                     /locus_tag="VF453_19190"
     CDS             803..1201
                     /locus_tag="VF453_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX7689840.1"
                     /translation="MPFTRISLLRGKPPAYLQAVSESLHRALVEAFEVPPDDRFQAIH
                     QHAPGELVFDRRYLGGPRGDDYLLVAVTAGRPRSADTKRAFYRRLVERLAESPGIAPR
                     EVMVVIGTTGAEDWSFGDGRMTMIDGALPC"
     gene            1195..>1563
                     /locus_tag="VF453_19195"
     CDS             1195..>1563
                     /locus_tag="VF453_19195"
                     /inference="COORDINATES: protein motif:HMM:NF027483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HEX7689841.1"
                     /translation="MLTMQYSLVLPAAFDMAAIRARIAAKGPLLDDFPGLAFKAYLYA
                     CRGEHGPENLYAPFYLWADTAAMTRFLSGPGFAALTGDFGWPVVKTAVPFAQELRSGL
                     RTARFATRELRPIAPHADLAA"
BASE COUNT          214 a          602 c          520 g          227 t
ORIGIN      
        1 cgcggatgcg cccgtgcgaa gcccgccagc acgccggtca gcaggccctc gccgaagtcc
       61 tcctgcaggc cgagccgcac ggcgccgtgc agctccgcgc cgcgcaccgc gtgggcggcc
      121 tcgtcatcga gcgcgagcag ccggcgcgcg tagccgaaca gcacctcgcc cgtcggcgtc
      181 agcaccatgc cgcggccggc cttgcgcagc accggcgcgc cggcggcctc ctcgagcttc
      241 ttcaactgcg cgctgacggc cgaggtcgag cgccccagcc ggtccgccgc cttggcgaag
      301 ctgcccagct cgatgccggt caccagcgtg cgcagcacgt cgaggtcgaa ggtggggcgg
      361 ctcatgcaat cgtcctgttt catcgaatga tcggtgccga attttcaagt tttcaaaact
      421 tcggtgcaac cgtatcgtgg agccatcccc ctcaccggag aacttcgatg acccagccct
      481 ccaccgtcgc ggcgcagatg tacggcgacg tcgcgcccaa gttcaccgag ctgaccgacc
      541 agctgctgtt cgacgacatc tggaagcgcc cgcagctgag cccgcgcgac cgcagcctgg
      601 ccacggtgtc ggcgctgatc gcgctgtacc ggctcgagca gttgccgttc cacctcgacc
      661 gcgcgctcga caacggcgtc acgcgcgagg agctcgccga gctcgtcgcc cacctcgcct
      721 tctatgccgg ctggccgtgc gcggcgtcgg ccaccaagct gctcgcggcg gccctggcca
      781 ggcgctgagc gcggaggcgg ccatgccctt cacgcgaatc tccctgctgc gcggcaagcc
      841 gccggcatac ctccaggccg tatcggagag cctgcaccgc gcgctggtcg aggccttcga
      901 ggtgccgccc gacgaccgct tccaggccat ccaccagcac gcgcccgggg agctcgtgtt
      961 cgatcgccgc tacctcggcg ggccgcgcgg cgacgactac ctgctcgtcg ccgtgacggc
     1021 cggccggccg cgctcggccg acaccaagcg cgccttctac cgccggctgg tggaacgcct
     1081 ggccgagtcg cccggcatcg cgccgcgcga ggtgatggtg gtgatcggca ccacgggcgc
     1141 cgaggactgg tccttcggcg acggcaggat gacgatgatc gacggagcgc tgccatgctg
     1201 acgatgcaat acagcctcgt gctgcccgcg gccttcgaca tggccgccat ccgcgcccgc
     1261 atcgcggcca aggggccgct gctcgacgac ttcccgggcc tcgcgttcaa ggcctacctc
     1321 tacgcctgcc gcggcgagca cgggccggag aacctgtacg cgccgttcta cctgtgggcc
     1381 gacaccgccg cgatgacgcg cttcctgtcc ggtccgggct tcgcggcgct gaccggcgac
     1441 ttcggctggc ccgtcgtcaa gaccgcggtg ccgttcgcgc aggaattgcg gtccggcctg
     1501 cgcacggccc ggttcgcgac gcgcgagctg cggccgatcg caccgcatgc cgacctcgcg
     1561 gcg
//
LOCUS       DASVLN010000809         3351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_152508, whole genome shotgun sequence.
ACCESSION   DASVLN010000809 DASVLN010000000
VERSION     DASVLN010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152508"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..2163
                     /locus_tag="VF453_19200"
     CDS             157..2163
                     /locus_tag="VF453_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEX7689842.1"
                     /translation="MSEADSSIQVELDSGKRVKVKAANVLLRFDKPSPAELLAQGQAM
                     AREIDLDLAWEFAGEQDFGFADLARDYFDASAGPVQQAAALFRLFEAPHYFRRMGKGA
                     FRKAPEEQVKAALLGIERKRQQALQIEAWAAELAAGRTPEPIREQLYRILFKPDKNAP
                     EYKAVVEASRQSQRSPLDLLKAAGAISSPYQFHWRRFLFEEFPRGTGFPPLEAPPIKD
                     ELPLAPVQAFSIDDSATTEIDDALSVQGLGTGTVVFGVHIAAPGLAIAPDSAVDKVAR
                     ERLSTVYMPGWKITMLPDGVVERYTLAEGRACPAVSLYASFDEATLALKSTETRLEAV
                     PIAANLRHDRLDGVVTEPALTGAEPADYPFAPELAFAFRLARHLKAAREVVRGKPENF
                     TRPDYNFRLERPEGGAPAEEPRGDERVVIGERKRGAPLDLIVSEAMILANSTWGGWIA
                     DMGVPGIYRSQASLAPGVKVRMGTKPQPHAGMGVAQYTWATSPLRRYVDLVNQWQIIA
                     CVRHGRMAPLSAPFKPKDATLFSIISGFDTAYAAYNGFQTGIERFWTLRWLQQNDVHE
                     LDAAVLKEGLVRAETLPLVFKAAGCDGLPRGTRVRVRVDGIDLLTLDLHTRLLARLDE
                     TGTAAAGESEDAEDGEDAAGAGPLTIAIDVDEPEPPAQGTSTPA"
     gene            2246..3151
                     /locus_tag="VF453_19205"
     CDS             2246..3151
                     /locus_tag="VF453_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7689843.1"
                     /translation="MFAPEPLSVRLRRWRPDGIQIGVIASALLHAAVFGAVGLYIALV
                     GVPEFNRVFQETPLEVVLVNARSKEAPTKAQAIAQATLAGGGEAESGLATSPLPPSPS
                     SLQGEQDTEAAQAIQQMQAEQQQLLTQLRRELAQMPVPDPRKLVLTQAERQQEEQRQQ
                     KLRTLAAIEKRVNEENSRPRRRYISPSTREEVYAVYYDALRHKIEERGTRDFPTLNGH
                     KLYGELTMNLTIDAQGRVVEAEIERSSNSRWLDQRAIAIARAAAPFGHFSDDMRRNAD
                     QIVVTSRFRFTKSDGLETTLTTSGG"
     gene            3156..>3351
                     /locus_tag="VF453_19210"
     CDS             3156..>3351
                     /locus_tag="VF453_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689844.1"
                     /translation="MTTSLRDAPAAPSAPAPAPVRLAVFGHPVSHSRSPQIHGRFAAA
                     CGHAIDYARIEAPLDGFVASV"
BASE COUNT          507 a         1278 c         1138 g          428 t
ORIGIN      
        1 ccgcgcgccg cgcagccccg gccgccgcgc gcccgcgccc ggcagttgcg tttcgctgcc
       61 ttttgccgcc cggggacgcc gccgaactcg gcgacaatcg tccccgtgaa ttacgctctc
      121 ttcgatgact ccggcaagtt cctcgccggt cgggtgatgt ccgaggcgga cagctcgatc
      181 caggtggagc tcgactccgg caagcgcgtc aaggtcaagg cggccaacgt gctgctgcgc
      241 ttcgacaagc cgtcccccgc cgaactgctc gcgcaaggcc aggccatggc gcgcgagatc
      301 gatctcgacc tcgcctggga attcgccggc gagcaggact tcggcttcgc cgatctcgcg
      361 cgcgactact tcgacgcctc cgcaggcccg gtgcagcagg ccgcggcgct gttccggctg
      421 ttcgaggcgc cgcactactt ccgccgcatg ggcaagggcg cgttccgcaa ggcgcccgag
      481 gagcaggtca aggcggccct gctgggcatc gagaggaagc gccagcaggc gctgcagatc
      541 gaggcctggg ccgcggagct cgccgccggc cgcacgcccg agccgatccg cgagcagctg
      601 taccgcatcc tgttcaagcc ggacaagaac gcgcccgagt acaaggccgt cgtcgaggcc
      661 tcgcggcaat cgcagcgctc gccgctggac ctgctgaagg ccgcgggagc gatctcgagc
      721 ccctaccagt tccactggcg ccgcttcctg ttcgaggaat tcccgcgcgg caccggcttc
      781 ccgccgctcg aggcgccccc catcaaggac gagctcccgc tcgcgccggt gcaggcgttc
      841 tcgatcgacg actccgccac gaccgagatc gacgacgcgc tctcggtgca gggcctgggc
      901 accggcacgg tcgtcttcgg cgtgcacatc gcggcgcccg gcctggccat cgcgccggac
      961 tcggccgtcg acaaggtcgc gcgcgagcgg ctgtccaccg tctacatgcc gggctggaag
     1021 atcacgatgc tgccggacgg cgtcgtcgag cgctacacgc tggccgaggg ccgcgcctgc
     1081 cccgcggtga gcctgtacgc cagcttcgac gaggcgacgc tggcgctgaa gtcgaccgag
     1141 acgaggctgg aggcggtgcc gatcgccgcc aacctgcgcc acgatcggct ggacggcgtg
     1201 gtcaccgagc cggcgctcac cggcgcagag cccgcggact acccgttcgc gcccgagctg
     1261 gcgttcgcct tccgcctcgc gcgccacctg aaggccgcgc gcgaggtcgt gcgcggcaag
     1321 cccgagaact tcacgcgccc ggactacaac ttccgcctcg agcgccccga gggcggtgcg
     1381 ccggccgagg agccgcgcgg cgacgagagg gtcgtcatcg gcgagcgcaa gcgcggcgcg
     1441 ccgctggacc tgatcgtgtc ggaagcgatg atcctcgcca acagcacgtg gggcggctgg
     1501 atcgcggaca tgggcgtgcc cggcatctac cgcagccagg cgagcctcgc gcccggcgtg
     1561 aaggtgcgca tgggcacgaa gcctcaaccg cacgcgggca tgggcgtcgc gcagtacacc
     1621 tgggcaacct cgccgctgcg gcgctatgtc gacctcgtca accagtggca gatcatcgcc
     1681 tgcgtccgcc acggccggat ggcgccgctg tcggcgccgt tcaagcccaa ggacgcgacg
     1741 ctgttctcga tcatctccgg cttcgacacc gcctacgcgg cctacaacgg cttccagacc
     1801 ggcatcgagc gcttctggac gctgcgctgg ctgcagcaga acgatgtcca cgagctcgat
     1861 gccgccgtgc tgaaggaagg actggtgcgc gcggagacgc tgccgctggt attcaaggcc
     1921 gccggctgcg acggcctgcc gcgcggcacg cgcgtgcgcg tgcgcgtcga cggcatcgac
     1981 ctgctcacgc tggacctgca cacccgcctg ctcgcgcgcc tcgacgagac cggcacggcg
     2041 gccgccggcg agtccgaaga cgccgaggac ggcgaggacg cggccggcgc cggcccactg
     2101 acgatcgcga tcgacgtcga cgagcccgag ccgcctgcgc aaggcacatc cacgccggcc
     2161 tagagtcccc gaccgctcca ccgaccgccc gacccgcccg cccacgccga tggacaagca
     2221 cttcacgccc ccgcccgcgc gcgagatgtt cgcgccggag ccgctctccg tgcgactgcg
     2281 ccggtggcgg cccgacggca tccagatcgg cgtcatcgcc tcggccctgc tgcatgccgc
     2341 ggtcttcggc gcggtcgggc tgtacatcgc gctcgtcggc gtgccggagt tcaaccgcgt
     2401 cttccaggag acgccgctgg aagtcgtgct ggtcaacgcg cgcagcaagg aggcgccgac
     2461 caaggcccag gcgatcgcgc aggcgacgct cgcgggcggc ggggaggccg agtcgggcct
     2521 agcgacctcg cccctgcccc cctcgcccag cagcctgcag ggcgagcagg acaccgaggc
     2581 cgcgcaggcg atccagcaga tgcaggcgga gcagcagcag ctcctgacgc aactgcgccg
     2641 cgagctcgcg cagatgcccg tgcccgaccc gcgcaagctc gtgctgacgc aggccgagcg
     2701 ccagcaggag gagcagcgcc agcagaagct gcgcacgctc gccgcgatcg agaagcgcgt
     2761 caacgaggag aactcgcgtc cccgccggcg ctacatcagc ccgtcgacgc gcgaggaggt
     2821 ctatgccgtc tactacgacg cgctgcggca caagatcgag gagcgcggca cgcgcgactt
     2881 tcccacgctc aacggccaca agctctacgg cgagctgacg atgaacctga ccatcgacgc
     2941 ccagggccgc gtcgtcgagg ccgagatcga gcgcagctcc aactcgcgct ggctcgacca
     3001 gcgcgccatc gcgatcgcgc gcgccgcggc ccccttcggc cacttcagcg acgacatgcg
     3061 ccgcaacgcc gaccagatcg tcgtcacctc gcgcttccgc ttcaccaagt cggacggcct
     3121 ggagacgacc ttgaccacga gcggaggctg acgccgtgac gacctccttg cgcgatgccc
     3181 ccgccgcgcc gtccgcaccc gcgccggcgc cggtgcgcct ggccgtcttc ggccacccgg
     3241 tctcgcacag ccgctcgccg cagatccacg gccggttcgc ggccgcgtgc gggcacgcca
     3301 tcgactacgc gcgcatcgag gcgccgctgg acggcttcgt cgccagcgtg c
//
LOCUS       DASVLN010000810         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_417538, whole genome shotgun sequence.
ACCESSION   DASVLN010000810 DASVLN010000000
VERSION     DASVLN010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417538"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VF453_19215"
     CDS             complement(<1..182)
                     /locus_tag="VF453_19215"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HEX7689845.1"
                     /translation="MSALLPESLAETAELDAAPPPAPLREFWRHFAASRGALAGLAVF
                     ALVVLAAVFAGVLASH"
     gene            complement(244..1272)
                     /locus_tag="VF453_19220"
     CDS             complement(244..1272)
                     /locus_tag="VF453_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7689846.1"
                     /translation="MLAYVLRRLALVVPTFLGITLLSFSLVHLIPGDPVQAMVGQHDV
                     DPAFRAEMRHRLGLDQPLWMQYGAYLWRALHLDLGESFFTHAPVWQEFASLFPATVEL
                     ATCAMLFAAVLGLLAGVLAAIRRGSALDHGVMAVSLTGYSMPIFWWGLLLIMFFAVAM
                     RDLAPALALPVSGRIALEYDVPARTGFMLVDAWLAPDEGAWRSAVSHLVLPSVVLGTI
                     PLAVIARMTRSAMLEVLREDYVRTARAKGLSPARIVFVHALRNALVPVLTMVGLLVGS
                     LLGGAVLTETLFSWPGVGKWLVDAVVRRDYPVVQGGILISATLVIAVNLLVDVLNAQV
                     NPRLRDVS"
     gene            complement(1280..>2086)
                     /locus_tag="VF453_19225"
     CDS             complement(1280..>2086)
                     /locus_tag="VF453_19225"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7689847.1"
                     /translation="AGECAVARVPLAEAGRLAGEGLAITRVKALGTSYVLPNANRPFL
                     SDKRLRQALSLAIDRASLVKTIYGGEGQVAGALLPPPMWGHDPSIANAYDPAQARALV
                     KASGYDGRELVLLMQANDHLVQRSVEMMMADWAKVGIKVVPRVLESNELFARIEAGDG
                     DLSFVAWYADNGDPDNFLSQQLACAGTGGTSKYHWCSAAFDKLLADARATTDLAARTA
                     LYRKAQAMIHDEAAEIPLAYATSAWAMGKRVSGVVVTPFLTVDFRGARLQ"
BASE COUNT          328 a          756 c          762 g          240 t
ORIGIN      
        1 tcgtgcgacg cgagcacgcc cgcgaacacc gcggccagca ccaccagcgc gaacaccgcg
       61 agccccgcga gcgcgccgcg gctggccgcg aagtggcgcc agaactcgcg cagcggcgcg
      121 gggggcggcg cggcgtcgag ctcggcggtc tcggcgaggg attccggcag cagggcggac
      181 atcacgtcac tcccgtcgtg ccgactgcga tgcgagcccc gtccggagcg gccgggcggg
      241 ggctcatgag acgtccctca gccgcggatt gacctgcgcg ttcagcacgt ccaccagcag
      301 gttgaccgcg atgaccagcg tcgccgagat caggatgccg ccctgcacca cgggatagtc
      361 gcgccgcacc accgcgtcga ccagccactt gccgacgccc ggccacgaga acagcgtctc
      421 ggtgagcacc gcgccgccca gcaggctgcc gaccagcagg ccgaccatcg tcagcaccgg
      481 cacgagcgcg ttgcgcagcg cgtgcacgaa gacgatgcgg gccggcgaca ggcccttggc
      541 gcgcgcggtg cggacgtagt cctcgcgcag cacctccagc atcgccgagc gcgtcatgcg
      601 cgcgatcacg gccagcggga tcgtgccgag cacgaccgac ggcagcacga ggtgcgagac
      661 cgcggagcgc caggcgcctt cgtccggcgc gagccaggcg tcgaccagca tgaagccggt
      721 gcgcgcgggc acgtcgtatt cgagcgcgat gcggcccgag accggcaggg cgagcgcggg
      781 ggcgaggtcg cgcatcgcca ccgcgaagaa catgatcagc agcaggcccc accagaagat
      841 gggcatcgag tagcccgtca gcgacacggc catcacgccg tggtcgagcg ccgagccgcg
      901 gcggatcgcg gccagcaccc ccgccagcag gcccagcacc gcggcgaaca gcatcgcgca
      961 cgtcgcgagt tccaccgtcg cggggaacag cgacgcgaac tcctgccaca ccggcgcgtg
     1021 cgtgaagaag gactcgccga ggtcgaggtg cagcgcccgc cacaggtacg cgccgtactg
     1081 catccacagc ggctggtcga ggcccaggcg gtgccgcatc tcggcgcgga aggcggggtc
     1141 gacgtcgtgc tggcccacca tcgcctgcac cgggtcgccg gggatcaggt gcacgagcga
     1201 gaacgatagc agcgtgatgc cgaggaaggt cggcaccacg agggcgaggc ggcgcaggac
     1261 gtacgcgagc atcgccgcgc tattgcagcc gcgcgccgcg gaagtcgacc gtcaggaacg
     1321 gcgtgacgac gacgcccgag acgcgcttgc ccatcgccca cgccgaggtc gcgtaggcga
     1381 gggggatctc ggccgcctcg tcgtggatca tcgcctgcgc cttgcggtag agcgcggtgc
     1441 gggcggcgag gtcggtggtc gcgcgcgcgt cggccagcag cttgtcgaag gccgcgctgc
     1501 accagtggta cttgctcgtg ccgccggtgc ccgcgcaggc gagctgctgc gacaggaagt
     1561 tgtccgggtc gccgttgtcg gcgtaccagg cgacgaacga caggtcgccg tcgccggcct
     1621 cgatgcgcgc gaacagctcg ttggactcga gcacccgcgg cacgaccttg atgccgacct
     1681 tcgcccagtc ggccatcatc atctcgacgc tgcgctgcac gaggtggtcg ttggcctgca
     1741 tcagcagcac gagctcgcgg ccgtcgtagc cgctcgcctt gaccagcgcg cgcgcctggg
     1801 ccgggtcgta cgcgttggcg atcgacgggt cgtggcccca catcggcggc ggcagcagcg
     1861 cgccggcgac ctggccctcg ccgccgtaga tcgtcttcac cagggacgcg cggtcgatcg
     1921 cgagcgacag cgcctggcgc aggcgcttgt cggacaggaa gggccggttc gcattgggca
     1981 gcacgtagct cgtgcccagc gccttcacgc gcgtgatcgc gaggccctcg cccgcgaggc
     2041 gcccggcctc ggccagcggc acgcgcgcga ccgcgcactc gcccgc
//
LOCUS       DASVLN010000811         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_473180, whole genome shotgun sequence.
ACCESSION   DASVLN010000811 DASVLN010000000
VERSION     DASVLN010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473180"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1124)
                     /gene="trpD"
                     /locus_tag="VF453_19230"
     CDS             complement(90..1124)
                     /gene="trpD"
                     /locus_tag="VF453_19230"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX7689848.1"
                     /translation="MPITDTEALTRVIEHREIFHDEMLALMRRIMSGEMSPTMIAAIA
                     IGLRVKKETIGEIAAAAQVMREFATGVPVADKARLVDIVGTGGDSSHTFNISTAASFV
                     AAAAGARVAKHGGRSVSSTSGSADVMEALGAHIDLSPEQVAVCLEETGFGFMFAPNHH
                     ASMKHAAPVRRELGVRTIFNILGPLTNPAGAPNQLMGVFHPDLVGIQVRVLQRLGSEH
                     VMVVYGMNGMDEVSLSGETMVGELKDGEVREYVVHPSDFGLPVYDSRVLRVANKDESV
                     ACIRRALGNEEGPVRDVVLLNAGAALYCAGAASSITDGVKRAREAVASGAAQAKLDQF
                     VALTRKLKGA"
     gene            complement(1159..2232)
                     /gene="ltaE"
                     /locus_tag="VF453_19235"
     CDS             complement(1159..2232)
                     /gene="ltaE"
                     /locus_tag="VF453_19235"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HEX7689849.1"
                     /translation="MSAMIDLRSDTVTRPTAAMRDAMFAAPLGDDVFGDDPSVNALQV
                     RIAALLGKEAALFMPTGTQSNLAALMAHCQRGDEYLVGQMAHTYRWEGGGGAVLGSIQ
                     PQPIAHQPDGSLALADIEAAIKPDDAHFARTRLLCLENTFGGQPLAAGYLREATALAR
                     RRGLATHLDGARLFNAAVHRAEHEGGDAYAHARAIADDFDSVSVCFSKGLGAPVGSAL
                     VGSRELIARAHRIRKMAGGGMRQAGILAAAAHHALDHHVDRLREDHANAKRLAAGLAG
                     LPGVSFAPPATNILFVDLAPELVRARVAARWGGAHDALVDHLRARGVLCTGLYKLRFV
                     THLDVDAAAIDRAIVEIRAALGG"
     gene            complement(2229..2819)
                     /locus_tag="VF453_19240"
     CDS             complement(2229..2819)
                     /locus_tag="VF453_19240"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX7689850.1"
                     /translation="MLLMIDNYDSFTFNLVQYFGELGEEVRVVRNDEITVEEIGAMRP
                     DHLVLSPGPCSPAEAGVCVPAIRAFIGKLPILGVCLGHQSIGAALGGRIVRAQRQMHG
                     KASTLSTDGEGVYAGLPREFSVVRYHSLAIERDTCPQELVVTSTSEDGEIMGVRHREL
                     AATATPLEGVQFHPESILSEHGHAMLRNFVAMGRVQ"
     gene            complement(3192..>4383)
                     /locus_tag="VF453_19245"
     CDS             complement(3192..>4383)
                     /locus_tag="VF453_19245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7689851.1"
                     /translation="RAASAAAGAVAAGAAALLLGGCASAPADVVKSAPVDLFDDAHVG
                     PPAEPVSPDDAFRVTPLMTAYIHDQILPLSHHGGERQALMEALYQRSKLRLEYEASMT
                     RNASQAFEARAGNCLALVMMTGAFAKAMGLNVTYQVVSAEETWSRAGGMYFMSGHVNL
                     TLTRPFVSQLERYDRVKDYTIDFMPAPEAATMRATPVSERTITAMYMNNRAAEAMLAG
                     HLDDAYWRVRDAIRLDPSYISAYNTLGVIYLRRHDAARAEAALHFALAHSPDNPRVMG
                     NYAQALHGVGRDGEAKALETRLAAIEPFPPFYFFNKAREAFALGRVAEARDLLHKEIA
                     REPDYHEFHYWLALCDFSLGDVDAARDELTTALDDADKRSDHDLYAAKLDRLKAWQQK
                     TSLQ"
BASE COUNT          580 a         1525 c         1634 g          644 t
ORIGIN      
        1 caggtcggcc gcgctgcgcc tcgggcgcgc ggcggcgatc tcctcgcgct tgacctcgac
       61 gatgcgttgc aggatgtccg acatcgcgct cacgccccct tcagcttgcg cgtgagcgcg
      121 acgaactggt cgagcttggc ctgcgccgcg ccgctggcca cggcctcgcg cgcgcgcttg
      181 accccgtcgg tgatcgacga ggccgcgccc gcgcagtaca gcgccgcgcc ggcgttcagc
      241 agcacgacgt cgcgcaccgg cccttcctcg ttgccgagcg cacgccggat gcaggcgacc
      301 gactcgtcct tgttggccac gcgcagcacg cgcgagtcgt agaccggcag gccgaagtcg
      361 ctcggatgga cgacgtactc gcgcacctcg ccgtccttca gctcgcccac catcgtctcg
      421 cccgacagcg acacctcgtc catgccgttc atgccgtaga cgaccatcac gtgctcggaa
      481 ccgaggcgct gcagcacgcg cacctggatg ccgaccaggt cgggatggaa cacgcccatc
      541 agctggttgg gcgcgcccgc cggattggtg agcgggccca ggatgttgaa gatggtgcgc
      601 acgcccagct cgcggcgcac gggcgcggcg tgcttcatcg acgcatggtg gttgggcgcg
      661 aacatgaagc cgaagccggt ctcctcgagg cagacggcga cctgctcggg cgagagatcg
      721 atgtgcgcgc cgagcgcctc catcacgtcc gcgctgccag aggtcgacga cacgctgcgc
      781 ccgccgtgct tggcgacccg cgcgcccgcg gccgccgcga cgaaggacgc cgcggtcgag
      841 atgttgaacg tgtgcgagct gtcgccgccg gtgcccacga tgtcgaccag ccgcgccttg
      901 tcggccaccg gcacgccggt ggcgaactcg cgcatcacct gcgcggcggc ggcgatctcg
      961 ccgatcgtct ccttcttcac gcgcaggccg atcgcgatcg ccgcgatcat cgtcggcgac
     1021 atctcgccgc tcatgatgcg gcgcatcagg gccagcatct cgtcgtggaa gatctcgcgg
     1081 tgctcgatga cgcgggtcag cgcctcggtg tcggtgatcg gcatggtcgt tccaggtcgg
     1141 tcgctgatgg gagggggctc agccgccgag cgcggcgcgg atctcgacga tcgcgcgatc
     1201 gatcgcggcg gcgtcgacgt ccaggtgcgt cacgaagcgc agcttgtaca ggccggtgca
     1261 cagcacgccg cgcgcgcgca ggtggtcgac gagggcgtcg tgcgcgccgc cccagcgcgc
     1321 cgcgacgcgc gcccgcacga gctcgggcgc gaggtcgacg aacaggatgt tggtcgccgg
     1381 cggcgcgaag ctcacgcccg gcaggccggc gaggcccgcg gccaggcgct tcgcgttggc
     1441 gtggtcctcg cgcaggcggt cgacgtggtg gtcgagggcg tggtgggccg ctgcggccag
     1501 gatgccggcc tgacgcatgc cgccgccggc catcttgcgg atgcggtgcg cacgggcgat
     1561 cagctcgcgc gagccgacca atgccgagcc caccggcgcg cccaggccct tgctgaagca
     1621 gaccgagacg ctgtcgaagt cgtccgcgat cgcgcgcgca tgggcgtagg cgtcgccgcc
     1681 ctcatgctcg gcgcggtgga cggcggcgtt gaacaggcgc gcgccgtcca ggtgcgtcgc
     1741 gaggccgcgc cggcgcgcga gcgcggtcgc ctcccgcagg tagccggccg cgagcggctg
     1801 gccgccgaac gtgttctcca ggcacagcag gcgggtgcgg gcgaagtggg cgtcgtcggg
     1861 cttgatcgcg gcctcgatgt cggccagcgc gagcgaaccg tcgggctgat gcgcgatcgg
     1921 ctgcggctgg atgctgccca acactgcgcc gccgccgccc tcccagcggt aggtgtgggc
     1981 catctggccg accaggtatt cgtcgccgcg ctggcaatgg gccatcagcg ccgcgaggtt
     2041 gctctgcgtg ccggtgggca tgaagagcgc cgcctccttg ccgagcagcg cggcgatgcg
     2101 cacctgcagc gcgttgacgc tggggtcgtc gccgaagacg tcgtcgccca gcggcgcggc
     2161 gaacatcgcg tcgcgcatcg cggcggtggg ccgcgtgacg gtgtcgctgc gcaggtcgat
     2221 catggcgctc attgcacgcg ccccatcgcg acgaagttgc gcagcatcgc gtggccgtgc
     2281 tcggacagga tggactcggg gtggaactgc acgccctcca gcggcgtcgc ggtggccgcg
     2341 agctcgcggt ggcgcacgcc catgatctcg ccgtcctccg aggtcgaggt gacgacgagt
     2401 tcctgcgggc aggtgtcgcg ctcgatcgcg agcgagtggt agcgcacgac gctgaactcg
     2461 cgcggcaggc ccgcgtagac gccctcgccg tccgtcgaca gcgtgctcgc cttgccgtgc
     2521 atctggcgct gcgcgcggac gatccgcccg ccgagcgccg cgccgatgct ctggtggccc
     2581 aggcacacgc cgaggatcgg cagcttgccg atgaaggccc ggatcgcggg cacgcacacg
     2641 ccggcctcgg cgggcgagca cggtccgggc gagagcacca ggtggtcggg ccgcatcgcg
     2701 ccgatctcct cgaccgtgat ctcgtcgttg cgaacgacgc gcacctcctc gcccagttcg
     2761 ccgaagtact ggacgaggtt gaaggtgaag gagtcgtagt tgtcgatcat cagaagcatg
     2821 gcaattcctg gcggtgtctg gcgaccgcgg cgctggagtc cgcctgggcc ggcggggcgc
     2881 ggtcatcttc cctgggtgga ccgccgcgcg gggcgcgtcc tggggcgggc gcgcgggcgg
     2941 cgactcgtgg cgtggacggc gagcgtcacc aacgccagtg ccgcaggcgg ccgagttccc
     3001 agagttgctt catccagaca ttctgacacg accgggccgc ggccgtggcg tgcgctcgcc
     3061 ctttcgagaa ggcatcctgc atgcgcgccg aaggctcgcg cgggcccgtg cggagcggtg
     3121 gcgcgagggc gcgcgccgcg ctcatggccg acggcgcgcg tccgtcgcga gggaacgcat
     3181 cggacgcccg ctcactgcag gctggtcttc tgctgccagg ccttgagccg gtcgagcttg
     3241 gcagcgtaga ggtcgtggtc ggagcgcttg tcggcatcgt ccagcgcggt ggtcagctcg
     3301 tcgcgcgcgg cgtcgacgtc gccgaggctg aagtcgcaca gggccagcca gtagtggaac
     3361 tcgtggtagt ccggctcgcg ggcgatctcc ttgtgcagca ggtcgcgcgc ctcggccacg
     3421 cggccgagcg cgaacgcctc gcgggccttg ttgaagaagt agaacggcgg gaacggctcg
     3481 atcgccgcga gccgcgtctc cagcgccttc gcctcgccgt cgcggcccac gccgtgcagc
     3541 gcctgggcgt agttgcccat gacgcgcggg ttgtccggcg aatgcgcgag cgcgaagtgc
     3601 agcgccgcct cggcgcgggc ggcgtcatgg cgacgcaggt agatcacgcc cagcgtgttg
     3661 taggcgctga tgtaggacgg atccagccgg atcgcgtcgc gcacgcgcca gtaggcgtcg
     3721 tcgaggtggc cggccagcat cgcctcggcg gcgcggttgt tcatgtacat cgccgtgatc
     3781 gtccgctcgc tgaccggcgt cgcacgcatc gtggccgcct cgggggcggg catgaagtcg
     3841 atcgtgtagt ccttgacgcg gtcgtagcgc tcgagctgcg agacgaaggg ccgcgtgagc
     3901 gtgaggttga cgtgcccgct catgaagtac atcccgccgg cgcggctcca ggtctcctcc
     3961 gcgctcacga cctggtaggt gacgttcagg cccatcgcct tggcgaaggc gccggtcatc
     4021 atcaccagcg ccaggcagtt gcccgctcgg gcctcgaagg cctgggaggc gttgcgcgtc
     4081 atcgaggcct cgtactcgag ccgcagcttg ctgcgctggt agagcgcctc catcagcgcc
     4141 tggcgctcgc cgccgtggtg cgacagcggc aggatctggt cgtggatgta ggccgtcatc
     4201 agcggggtga cgcggaaggc gtcgtcggga ctgaccggct cggccggcgg gccgacgtgg
     4261 gcgtcgtcga acaggtcgac cggcgccgac ttgacgacgt cggcgggcgc ggaagcacag
     4321 ccgcccagca gcaacgccgc ggcgccggcg gccaccgccc ccgcggccgc cgaggccgcc
     4381 cgg
//
LOCUS       DASVLN010000812         3637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_96025, whole genome shotgun sequence.
ACCESSION   DASVLN010000812 DASVLN010000000
VERSION     DASVLN010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96025"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VF453_19250"
     CDS             complement(<1..334)
                     /locus_tag="VF453_19250"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7689852.1"
                     /translation="MTAFAFVFPGQGSQAVGMLDAWGDHPAVASTLREASAALGEDIG
                     RLIHEGPKEQLDLTTNTQPVMLTAGIAAYRAWCAEGGATPAAVAGHSLGEYTALVAAG
                     VLTLADALP"
     gene            complement(400..1383)
                     /locus_tag="VF453_19255"
     CDS             complement(400..1383)
                     /locus_tag="VF453_19255"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7689853.1"
                     /translation="MSAQRFSRVAGTGSDLPPLRLTNADLVERLARRGVETSDEWIVE
                     RTGIRARHFVADDVHASDLAVAAARRALEAAGRPAEDVDLIIVATSTPDMVFPSTASI
                     VQRKLGVHGCPAFDVQAVCSGFIYALSVADAMIRVGTARCALVIGAEVFSRLLDFDDR
                     TTCVLFGDGAGAVLLEASDAPGVVATKLHADGRHLDILCTPGTVSGGKVFGDPLLKMD
                     GPAVFKLAVGVLGEVAHEVLELAGRTTSDVDWLVPHQANIRIMQSTAKKLKLPAERVV
                     VTVGEHGNTSAASIPLALDVAVRSGQIRRGQRLLLEGVGGGFTWGAALVDY"
     gene            complement(1380..2474)
                     /gene="plsX"
                     /locus_tag="VF453_19260"
     CDS             complement(1380..2474)
                     /gene="plsX"
                     /locus_tag="VF453_19260"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX7689854.1"
                     /translation="MIHPVPLTPRTRVRLAVDCMGGDFGPSVTLPACKSFLDSHPDAE
                     LLLVGSPGALAPATDWPRCTLITASQVVTMDDTVETALRRKRDSSMRVAIEQLKANEA
                     GAVAADACVSAGNTGALMAVARYLLKTMDGIDRPAIAAFLPHVNDGFTCVLDLGANVD
                     CSAEHLLQFAVMGSALVTAIQGKPDPSVGLLNVGEELIKGSEVIKRAGEMLRAVAEGE
                     HLNYHGNVEGDDIFKGTTDIVVCDGFVGNVMLKTSEGAAKMIRTMIKEEFERNWLSKL
                     VGALAKAVALGRFGKRVDPRRYNGAALLGLKGLVFKSHGGADVYSFGQALKRAHDAAR
                     NRLLDGVHDRIVETMQALPPTSGESVAQAA"
     gene            complement(2821..3003)
                     /gene="rpmF"
                     /locus_tag="VF453_19265"
     CDS             complement(2821..3003)
                     /gene="rpmF"
                     /locus_tag="VF453_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX7689855.1"
                     /translation="MAVQQNKKSPSKRGMHRSHNALTTPGTAIEPTTGEVHLRHHISP
                     TGFYRGRKVLKTKADA"
     gene            complement(3197..>3637)
                     /locus_tag="VF453_19270"
     CDS             complement(3197..>3637)
                     /locus_tag="VF453_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HEX7689856.1"
                     /translation="AAAEPGRLVRWRAVGSTRVVGGEAQRWLSLTASATVVLQCQRCL
                     QPMDEAVHVDRHFRFVRDEATAAALDEEMEDEVLELPRSLDLRELVEDEMLLALPLVP
                     RHAACPQPLPRAFGDVEEVEEAEQKSNPFAALAALKKGGAGEAH"
BASE COUNT          513 a         1256 c         1353 g          515 t
ORIGIN      
        1 gcggcagcgc gtcggccagc gtcagcacgc cggccgcgac gagcgccgtg tactcgccga
       61 gcgagtgccc cgccaccgcc gccggcgtcg cgccgccctc ggcgcaccag gcccggtagg
      121 ccgcgatgcc cgcggtcagc atgaccggct gggtgttggt cgtgaggtcg agctgctcct
      181 tcgggccctc gtggatcagg cggccgatgt cctcgccgag cgcggccgag gcctcgcgca
      241 gcgtcgacgc gacggccgga tggtcgcccc aggcgtcgag catcccgacg gcctgcgagc
      301 cctggccggg gaagacgaat gcgaatgcgg tcatggtctt tgttgttgtg tctcgtgtgg
      361 aagggcgccg gcgggtgggg ccggcgaaac ggcggcgcgt cagtagtcga cgagcgccgc
      421 gccccaggtg aagccgccgc ccacgccttc cagcagcagg cgctggccgc gccggatctg
      481 gcccgaacgc accgccacgt ccagcgccag cgggatcgag gcggccgagg tgttgccgtg
      541 ctcgcccacc gtgacgacga cgcgctccgc gggcagcttc agcttcttgg cggtgctttg
      601 catgatacgg atgttcgcct ggtggggcac cagccagtcg acgtccgagg tcgtgcgccc
      661 ggcgagttcc agcacctcgt gcgcgacctc gcccagcacg cccacggcga gcttgaagac
      721 ggccgggccg tccatcttca gcagcgggtc gccgaagacc ttgccgccgc tgacggtgcc
      781 gggcgtgcac aggatgtcga gatggcgccc gtcggcgtgc agcttggtgg cgacgacgcc
      841 gggcgcgtcg ctcgcctcca gcagcaccgc gcccgcgccg tcgccgaaca ggacgcaggt
      901 cgtgcggtcg tcgaagtcca gcaggcggga gaagacctcc gccccgatca ccagcgcgca
      961 gcgcgcggtg ccgacgcgga tcatcgcgtc ggccacgctc agcgcgtaga tgaagcccga
     1021 gcacaccgcc tgcacgtcga acgccgggca gccgtgcacg cccagcttgc gctggacgat
     1081 gctggccgtc gacgggaaca ccatgtccgg cgtggaggtc gcgacgatga tgaggtcgac
     1141 gtcctcggcc gggcggcccg cggcctcgag cgcgcggcgc gcggcggcga cggcgaggtc
     1201 gctcgcgtgg acgtcgtccg cgacgaagtg gcgggctcgg atgccggtgc gctcgacgat
     1261 ccactcgtcc gaggtctcga cgccgcgccg ggcgaggcgt tcgacgaggt cggcgttggt
     1321 caggcgcagc ggcggcaggt cgctgccggt gcctgcgacg cgggagaagc gttgagcgct
     1381 catgccgcct gcgcgacgga ttcgccgctc gtcgggggaa gcgcctgcat cgtctcgacg
     1441 atgcggtcgt gcacgccatc gagcagccgg ttgcgggccg cgtcgtgcgc gcgcttgagc
     1501 gcctgcccga acgagtagac gtcggcgccg ccgtggctct tgaacacgag gcccttcagg
     1561 cccagcagcg cggcaccgtt gtagcggcgc gggtcgaccc gcttgccgaa gcggccgagc
     1621 gcgaccgcct tcgcgagcgc gcccaccagc ttcgagagcc agttgcgctc gaattcttcc
     1681 ttgatcatcg tgcggatcat cttggccgcg ccttcgctgg tcttcagcat cacgttgccg
     1741 acgaagccgt cgcagacgac gatgtcggtc gtgcccttga agatgtcgtc gccttcgacg
     1801 ttgccgtggt agttgaggtg ctcgccttcg gccaccgcgc gcagcatctc gcccgcgcgc
     1861 ttgatgacct cgctgccctt gatcagctcc tcgccgacgt tgagcaggcc gacggacggg
     1921 tcgggcttgc cctggatcgc cgtcaccagc gcgctgccca tgacggcgaa ctgcagcagg
     1981 tgctccgcgc tgcagtcgac gttggcgccg aggtcgagga cgcaggtgaa gccgtcgttg
     2041 acatgcggca ggaaggccgc gatggcggga cggtcgatgc cgtccatcgt cttgagcagg
     2101 tagcgcgcga ccgccatcag cgcgcccgtg ttgcccgccg agacgcaggc gtccgccgcc
     2161 acggcgccgg cctcgttggc cttgagctgc tcgatggcga cgcgcatcga ggagtcgcgc
     2221 ttgcggcgca gggcggtctc gaccgtgtcg tccatcgtga cgacctggct ggccgtgatc
     2281 agcgtgcagc gcggccagtc ggtcgcgggc gccagcgcgc cggggctgcc gaccagcagc
     2341 agctcggcgt cgggatggct gtccaggaag gacttgcacg ccggcagcgt gacggacggc
     2401 ccgaagtcgc cgcccatgca gtccaccgcc aggcggacgc gcgtgcgggg tgtcaggggt
     2461 acagggtgga tcacggtcgg gatcggtcag ggccgatctg atttccagca acccgagacc
     2521 atagcagacg ctggtgacac gggcccgctg aacaacccga atgaccccgc cggacagccg
     2581 aggcggagac gaccgcgcct cgcgcttgcg gcgcgagagg acggcggatg cggggatggg
     2641 cggggccggc gcgcagcgct ggggccgaag gcacaaaccg acatgaacag gaagccgggc
     2701 gtcgaccggc ggtgccgcgc gcgcggcatc cggagggatc gacgacgatg ccgggtcatg
     2761 ctgcgcgcgg cgccggccgg gaaggcggca tgcacgcgcg cgagcgggag acccgcagga
     2821 tcaggcgtcg gccttggtct tcaggacctt gcggccacgg tagaagccgg tcgggctgat
     2881 gtggtggcgc aggtgcactt cgccggtggt cggctcgatc gcggtgcccg gggtggtcag
     2941 cgcgttgtgc gagcggtgca tgccgcgctt ggagggcgac ttcttgtttt gctggacggc
     3001 catgtggaac tcctggaatg tcggcgccgg ggtcggaaaa ccgggagtgt ccggcggatc
     3061 gacaatcggc aatgccgcag gagaaggctc gttgcccgaa ggaaacggca gtcctgcgac
     3121 gtcgttgaat gcggaaaagc ctagcagtat agcacgagca ggcggcaagt cgccaagctc
     3181 cgtccgcgcc ggcgattcag tgcgcctcgc ccgccccgcc cttcttcagc gcggccagcg
     3241 ccgcgaacgg gttcgacttc tgctcggcct cctcgacctc ctccacgtcc ccgaacgcgc
     3301 gcggcagcgg ctgcggacag gcggcgtgtc gcgggacgag cggcagggcg agcagcatct
     3361 cgtcctcgac cagctcgcgc aggtccagcg agcgcggcag ctcgagcacc tcgtcctcca
     3421 tctcctcgtc cagcgccgcg gcggtggcct cgtcgcggac gaagcggaaa tggcggtcga
     3481 cgtgcaccgc ctcgtccatc ggctgcaggc agcgctggca ctgcagcacg accgtcgcgc
     3541 tggcggtcag cgagagccag cgctgcgcct cgccgccgac cacgcgggtc gagccgaccg
     3601 cgcgccagcg caccaggcgc cccggctcgg cggcggc
//
LOCUS       DASVLN010000813         4391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_527438, whole genome shotgun sequence.
ACCESSION   DASVLN010000813 DASVLN010000000
VERSION     DASVLN010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527438"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..782
                     /locus_tag="VF453_19275"
     CDS             129..782
                     /locus_tag="VF453_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7689857.1"
                     /translation="MKTDKQLKSDVEAELDWDPSIDAAHVGVAVRDGVVTLTGHLNTF
                     AEKTAIERVVARVAGVRAVAVELDVKVASEHVRSDTEIAQAAQDAIAWNVVVPRDKVR
                     VKVEKGWLTLSGEVDWDYQRNAAATAVRTLKGVLGVTNSITLKQRVVPSDIGARIGTA
                     LARHAYREAAAIQVNVSGSSVTLRGKVDSWAERAAAEGAAWSAPGVTGVVSELTIGR"
     gene            1090..2928
                     /gene="ftsH"
                     /locus_tag="VF453_19280"
     CDS             1090..2928
                     /gene="ftsH"
                     /locus_tag="VF453_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7689858.1"
                     /translation="MDRKQAWSFALPLLMLVFLALMSVLGAPGPGPQQVSYDAFEHAL
                     AQGRIDRIVASDTTLVGHLKKPESGHAELVANRVEPELAARLDRFGVPYERVVQSGWW
                     RDLASWLLPTALFFLLWSAAMRRVGGRGGLGGFMGAGASSAKVHVENDTGVTFADVAG
                     VDEAKAELQEVVEFLGRPKEFERLGARVPRGVLLVGPPGTGKTMLAKAVAGEAGVPFF
                     SISGSEFVEMFVGVGAARVRDLFRQARERAPAIVFIDELDALGRARGIVSGFGGNDEK
                     EQTLNQLLAEMDGFDTSAGVVMLAATNRPEVLDPALLRAGRFDRQVLVDRPDRAGRLA
                     ILRVHVRKIRISPELALDDIAGLTVGFSGADLANLVNEAALVATRRKGELVAQDDFTQ
                     ALERIVAGLEKRSRVLNTKERERVAWHEMGHALVGIVLGGTDRVRKVSIIPRGIGALG
                     YTLQSPLEDRYLMSSTELESRLAVLLGGRAAECLVFGESSTGAADDLAKATDIALDMV
                     ARQGMDEALGPVAYERDGAPQLAGPARPWNERHAVSEDTRRRIDAAVRALLSRAMDRA
                     TRQLREHRAALDRCARALLERETLDDEAIRALAPELWGTAPLSRAA"
     gene            3071..3685
                     /locus_tag="VF453_19285"
     CDS             3071..3685
                     /locus_tag="VF453_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX7689859.1"
                     /translation="MLRTPCLAVLAAAALPILACAQPADSGDWIVRVRATHLQPRNDD
                     GTGLGLSINAKTLPELDITAFLTPQVASELVLTYPQRQTISAGGARIGSLKHLPPMLT
                     LQYHVLGMGPWRPYLGAGVNWTHFSDVAFDAATTQALHPTIAKDSFGPVLQAGADLPL
                     GRGWLLNADAKRAWIATDVKSSGTKVGTFRIDPWLLSVGVGYRF"
     gene            3739..4341
                     /locus_tag="VF453_19290"
     CDS             3739..4341
                     /locus_tag="VF453_19290"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7689860.1"
                     /translation="MTASASTSDKEGAPLAEPHLPDLPLQQALQARRSTRDFLPFELT
                     PGQLSSLLWAAAGVNRRASGGRTMPSAHGWQEVDVYAVTAQGAWRYDASAHRLLPVAP
                     GDLRASTGEQDFVGSAPLNLVYVVDFDRMPEAAPQEREFLCGVSAGAMAQNAYLAAAA
                     LGLGSVVRGLIDRRHLAASLGLRTTQRVLLAQTFGVPRLG"
BASE COUNT          597 a         1605 c         1613 g          576 t
ORIGIN      
        1 ctgagccctc gcgggcgccg gcccgcgacg cgcccgcgcg agcgggatcg cccccttgcg
       61 cgtgctcaac cccgctgcgc gcggcgacgc gaggatgggg attccaccca ctgctcgaag
      121 gagtcaccat gaagaccgac aagcaactca aatccgacgt cgaggccgag ctcgactggg
      181 atccgtcgat cgatgcggcc cacgtgggcg tcgccgtccg cgacggcgtc gtgacgctga
      241 ccgggcatct caacaccttc gcggagaaga ccgcgatcga gcgcgtggtg gcgcgggtcg
      301 cgggcgtgcg cgcggtcgcg gtggaactcg acgtcaaggt cgcgtccgag cacgtgcgca
      361 gcgacaccga gatcgcgcag gccgcgcagg acgcgatcgc ctggaacgtc gtcgtgccgc
      421 gcgacaaggt gcgcgtgaag gtcgagaagg gctggctcac gctcagcggc gaggtcgact
      481 gggactacca gcgcaacgcc gcggcgacgg cggtgcgcac gctcaagggc gtcctcggcg
      541 tgaccaactc gatcacgctc aagcagcggg tcgtgccctc ggacatcggc gcgcgcatcg
      601 gcaccgcact cgcgcggcac gcctaccgcg aggcggccgc gatccaggtc aacgtcagcg
      661 gctcctcggt gacgctgcgg ggcaaggtcg actcgtgggc cgagcgcgcc gcggccgagg
      721 gggccgcgtg gtcggcgccg ggcgtgacgg gcgtcgtcag cgagctgacg atcgggcgtt
      781 gagggcgacg ggccatgcga cgcggacatt gagcgcgcgc aagtcccccg tgcgaccgcc
      841 cgccagaatc gaccctgcca ccccgccagg gtcgaacgcc cgttgaatcg gcaggggcga
      901 cgaggtgagg gccgcgtcct cggccgttca atcccggcga ttcgcgccag gatcgtcgcc
      961 cgccgcgatg caggcggcct gcgcgagcag ggaccctgcg gggtggcgcc acgccgggcg
     1021 cgtcccggcc acacgaaacg gcgggcgcgc ggtcgggcgc atcgccagca atcatcggag
     1081 cgagcccgca tggatcgcaa gcaggcctgg agcttcgcgc tgccgctcct catgctcgtc
     1141 ttcctcgcgc tgatgagcgt gctgggcgcg ccagggccgg gcccgcagca ggtgtcgtac
     1201 gacgcgttcg agcatgcgct ggcgcagggc cggatcgatc gcatcgtcgc cagcgacacg
     1261 acgctggtcg ggcacctgaa gaagccggag tccgggcatg ccgagctcgt cgcgaaccgg
     1321 gtcgagcccg agctggcggc gcggctcgac cgcttcggcg tcccgtacga gcgcgtcgtg
     1381 cagagcggct ggtggcgcga cctcgcgtcc tggctgctgc cgacggcgct gttcttcctg
     1441 ctctggtccg ccgcgatgcg tcgcgtgggc ggtcgcggcg gcctgggcgg cttcatgggc
     1501 gccggcgcca gcagcgcgaa ggtccacgtg gagaacgaca ccggcgtgac cttcgccgac
     1561 gtggccggcg tcgacgaggc caaggcggag ctccaggaag tcgtcgagtt cctggggcgg
     1621 cccaaggaat tcgaacgcct gggcgcccgc gtcccgcggg gcgtcctgct ggtcgggccg
     1681 cccggcacgg gcaagacgat gctggccaag gcggtcgccg gcgaggcggg cgtgccgttc
     1741 ttctccatct ccggcagcga gttcgtcgag atgttcgtgg gcgtcggcgc ggcccgcgtg
     1801 cgcgacctgt ttcgccaggc ccgcgagcgc gcaccggcga tcgtcttcat cgacgagctc
     1861 gacgcgctgg gccgcgcgcg cggcatcgtc tccggcttcg gcggaaacga cgagaaggag
     1921 cagacgctca accagctgct cgccgagatg gacggcttcg acacctcggc aggcgtcgtc
     1981 atgctggcgg cgacgaaccg ccccgaggtg ctcgaccccg cgctgctgcg cgccggccgc
     2041 ttcgaccgcc aggtgctcgt cgaccggccg gatcgagccg ggcgcctcgc catcctgcgc
     2101 gtgcacgtcc gcaagatccg catctcgccg gagctggcgc tcgacgacat cgccggcctg
     2161 accgtcggct tcagcggcgc cgacctcgcc aacctggtca acgaggcggc gctcgtcgcg
     2221 acgcggcgaa agggcgagct cgtggcccag gacgacttca cgcaggcgct ggagcgcatc
     2281 gtcgcgggcc tcgagaagcg aagccgcgtg ctcaacacga aggagcgcga gcgggtcgcg
     2341 tggcacgaga tggggcacgc gctcgtgggc atcgtgctcg ggggaacgga tcgcgtgcgc
     2401 aaggtgtcca tcattccccg cggcatcggc gccctcggct acacgttgca gagcccgctc
     2461 gaggaccgct acctgatgtc cagcaccgag ctggagtcgc ggctcgcggt gctcctcgga
     2521 gggcgggcgg cggaatgcct ggtcttcggc gagagctcga ccggcgcggc cgacgacctc
     2581 gccaaggcga ccgacatcgc gctcgacatg gtcgcgcgcc agggcatgga cgaggcgctg
     2641 ggcccggtgg cctacgagcg cgacggcgcg ccgcagctgg ccggcccggc gcggccctgg
     2701 aacgagcggc atgccgtgtc ggaggatacc cggcgccgta tcgacgccgc cgtgcgggcg
     2761 ctgctgtctc gcgcgatgga ccgcgcgacc cggcagctgc gcgagcaccg tgcggcgctc
     2821 gatcgctgcg cccgggcgct gctggagcgc gagaccctgg acgacgaggc catccgcgcg
     2881 ctggcgcccg agttgtgggg cactgcgccg ttgtcgcggg ccgcgtgagg cggggcgatc
     2941 ccgccgccga tcccgccgcc gatcccgccg ccgattgcgc cgccgattgc gccgccgatt
     3001 gcgctgcggc aatgcagcgc ggccgagccg gcgctgcaat cgaggcatcc aaccacagga
     3061 gatccgaacc atgcttcgca ccccttgcct ggccgtcctc gccgccgccg cgctcccgat
     3121 cctggcgtgc gcccagcccg ccgattcggg cgactggatc gtccgcgtcc gggcgacgca
     3181 cctgcagccg cgcaacgacg acggcacggg cctcgggctg tctatcaacg cgaagacgtt
     3241 gcccgagctg gacatcacgg cgttcctcac cccgcaggtg gcctccgagc tcgtgctgac
     3301 gtatccgcag cggcagacga tctccgccgg cggcgcccgg atcgggtcgc tcaagcacct
     3361 gccgccgatg ctgacgctgc agtaccacgt gctcggcatg ggcccctggc ggccgtacct
     3421 gggagccggg gtcaactgga cgcacttctc cgacgtcgcc ttcgacgcgg ccacgaccca
     3481 ggcgctgcac ccgacgatag ccaaggacag cttcggcccc gtgctgcagg ccggcgccga
     3541 cctgcccctg ggccgcggct ggctgctcaa cgcggacgcg aagcgggcgt ggatcgcgac
     3601 cgacgtcaag tcgtcgggca cgaaggtcgg caccttccgg atcgacccgt ggctgctgtc
     3661 cgtcggcgtc ggctaccgct tctgaacccc cttgacgcgc gggcgaccgc gtcgcgcgtc
     3721 ccagcccgga gaccgcccat gaccgcttcc gcctcgacgt ccgacaaaga gggtgcgccg
     3781 ctcgccgagc cgcacctgcc cgacctgccg ctgcagcagg cgctgcaggc gcgccggtcg
     3841 acgcgggact tcctgccgtt cgagctcacg ccgggccagc tgtcgtcgct tctctgggcc
     3901 gccgccgggg tcaaccggcg cgccagcggc ggccggacga tgccatcggc gcacggctgg
     3961 caggaggtcg acgtctacgc cgtgaccgcc cagggcgcat ggcgctacga cgcatcggcg
     4021 catcgactgc tgcccgtcgc gcccggtgac ctgcgcgcaa gcaccggcga gcaggacttc
     4081 gtcggctcgg cgccgctgaa cctggtctac gtcgtcgact tcgaccggat gccggaggcc
     4141 gcgccgcagg agcgcgagtt cctgtgcggc gtcagcgccg gcgccatggc gcagaacgcc
     4201 tacctcgccg cggccgcgct gggcctgggc agcgtggtgc gcgggctcat cgatcgccgc
     4261 cacctcgcgg cgtcgctcgg gctgcggacg acgcagcgcg tgctgctcgc ccagaccttc
     4321 ggcgtcccgc gcctgggctg acgggcgcga ccgcgcacgc gagcggcatg caagccgtga
     4381 cgtcgcccgg c
//
LOCUS       DASVLN010000814         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_152857, whole genome shotgun sequence.
ACCESSION   DASVLN010000814 DASVLN010000000
VERSION     DASVLN010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152857"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..543
                     /gene="rplP"
                     /locus_tag="VF453_19295"
     CDS             127..543
                     /gene="rplP"
                     /locus_tag="VF453_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX7689861.1"
                     /translation="MLQPARRKFRKEQKGRNTGIATRGAAVSFGEFGLKATERGRITA
                     RQIEAARRAISRHIKRGGRIFIRIFPDKPISQKPAEVRMGNGKGNPEYYVAEIQPGKV
                     LYEINGVPEELAREAFTLAAAKLPLRCTFVSRQVGA"
     gene            555..758
                     /gene="rpmC"
                     /locus_tag="VF453_19300"
     CDS             555..758
                     /gene="rpmC"
                     /locus_tag="VF453_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX7689862.1"
                     /translation="MTKASELRTKDVPALEKEVSDLLRAHFGLRMQKGTQQLTNHSQL
                     KTTRRDIARAKTILAEKKKGAAK"
     gene            755..1033
                     /gene="rpsQ"
                     /locus_tag="VF453_19305"
     CDS             755..1033
                     /gene="rpsQ"
                     /locus_tag="VF453_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX7689863.1"
                     /translation="MTEAQAKPQTNTRTLIGRVVSDKRSKTITVLVERRHTHELYGKI
                     VGRSRKYHAHDEKGEYHMGDLVEIAEGRPISKTKSWVVTRLVEKARLV"
     gene            1246..1761
                     /locus_tag="VF453_19310"
     CDS             1246..1761
                     /locus_tag="VF453_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7689864.1"
                     /translation="MIQVGDKLPAATLKEFIEVETEGCALGPNDVDVAKATAGKTIAL
                     FALPGAFTPTCSAKHVPGYLAEVDALRAAGVDEVWCVSVNDAFVMGAWARDQRTAGKV
                     RMLGDGSAAFTKAVGLPLDLTDRGMGVRSQRYSMLVVDGVVRTLNVEAPGKFEVSDAA
                     TMLKQAQAARG"
     gene            2049..>2123
                     /locus_tag="VF453_19315"
     CDS             2049..>2123
                     /locus_tag="VF453_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HEX7689865.1"
                     /translation="MIQMQSRLDVADNTGAKSVMCIKVL"
BASE COUNT          386 a          735 c          704 g          298 t
ORIGIN      
        1 ccgcgcgccg aagccggtgc gggcgcccct ggcgacgcca agcctgccgc caagcgcgtc
       61 gtgcgcaagg ccgctgctcc gacggccgcg gctccggccg ctgccgaagg caaaggagaa
      121 taagacatgc tgcaacccgc acgtcgcaag ttccgcaagg aacagaaggg ccgcaacacc
      181 ggcatcgcca ctcgtggcgc cgccgtctcg ttcggtgaat tcggcctgaa ggccaccgag
      241 cgcggccgca tcaccgctcg ccagatcgaa gcggcccgtc gtgccatctc gcgccacatc
      301 aagcgcggtg gacggatctt catccgcatc ttcccggaca agccgatctc ccagaagccg
      361 gccgaagtcc ggatgggtaa cggcaagggc aacccggagt actacgtggc tgagatccag
      421 ccgggcaagg tgctctacga gatcaacggc gttccggaag aactggcgcg cgaggcgttc
      481 acgctcgcgg ccgccaagct gccgctgcgc tgcaccttcg tcagccgcca ggtcggcgcc
      541 tgataggagc acacatgacc aaagcatctg aactgcggac caaggacgtc ccggcgctcg
      601 agaaggaagt ctccgacctc ctgcgcgcgc acttcggcct gcgcatgcag aagggcaccc
      661 agcagctgac gaaccattcg cagctgaaga cgacccgtcg cgacatcgcc cgcgccaaga
      721 ccatcctggc cgagaagaag aagggggctg cgaagtgacc gaagctcaag ccaagccgca
      781 aacgaacacg cgcaccctga tcggccgcgt ggtcagcgac aagcgttcga agaccatcac
      841 ggtgctcgtc gagcgccgcc acacccacga gctgtacggc aagatcgtcg gccgctcgcg
      901 caagtaccac gcccacgacg agaagggcga gtaccacatg ggcgacctgg tcgagatcgc
      961 cgaaggccgt cccatctcga agaccaagag ctgggtcgtg acccgcctgg tcgagaaggc
     1021 tcgcctggtc tgatcccagc cgtcgggcgg gccgcaaggt ccgtcgccgg ctgcacggcc
     1081 ggcatgtcgg aatccccggc atgccggccg tttcccattt cgacgcgaca atcccgggcg
     1141 cgcggccgca accgtcgctg cgtcggcatc gtctgcggcc gcggctcggt gccggtgccg
     1201 cgcggcgacc gtcgcgtcac gtccatccac acgagaggag cccccatgat ccaggtcgga
     1261 gacaagttgc ccgccgccac gctcaaggaa ttcatcgagg tcgagaccga gggctgcgcg
     1321 ctcggtccca acgacgtcga cgtcgcgaag gcgaccgccg gcaagacgat cgcgctgttc
     1381 gcgctgcccg gcgcgttcac gccgacctgt tcggccaagc acgtgccggg ctacctggct
     1441 gaggtcgacg ccctgcgcgc cgccggggtc gacgaggtct ggtgcgtgtc cgtcaacgac
     1501 gccttcgtga tgggcgcctg ggcgcgcgat cagcgcacgg ccggcaaggt gcgcatgctg
     1561 ggcgacggct cggccgcctt cacgaaggcc gtcggcctgc cgctggacct gactgatcgc
     1621 ggcatgggcg tgcgctcgca gcgctactcg atgctggtcg tcgacggcgt ggtgcgcacg
     1681 ctcaatgtcg aggcgccggg caagttcgag gtgagcgacg cggcgacgat gctcaagcaa
     1741 gcacaggccg cccgcggctg aacgcgggca agctggcgcc gaagggtgcc ccgtcttttc
     1801 gacgatgggc gtttgacagc gccagcgcac cgcgtataca atgcgcagct tcgcccaaga
     1861 ggcctggaga gtttgcgccg tcgctccccg cgagtgatgc ccggttggat ccggaaggcg
     1921 cgcgcccttc gtctcgacgg catttctgcc caaacgggtc gactcggcca gcaggccgcg
     1981 acgttccgac gggcccaata ctgaccgctc tcgggttgcc gtctgcggga gcaagttggg
     2041 gataaaacat gattcaaatg cagtcgcgac tcgacgtcgc cgacaacacg ggcgcgaagt
     2101 ccgtcatgtg catcaaggtg ctc
//
LOCUS       DASVLN010000815         5862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_527624, whole genome shotgun sequence.
ACCESSION   DASVLN010000815 DASVLN010000000
VERSION     DASVLN010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527624"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF453_19320"
     CDS             complement(<1..248)
                     /locus_tag="VF453_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7689866.1"
                     /translation="MIAFHFDAHMLPLPEGHPFPVGKYGALRRRLQAEPGGIVLRPAP
                     AASEGELALAHDPGYIADVLGGTLDAARQREIGLPWTPA"
     gene            408..980
                     /locus_tag="VF453_19325"
     CDS             408..980
                     /locus_tag="VF453_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX7689867.1"
                     /translation="MLTAEQLLAAQKANLETLFELTNKAFEGVEKLVELNLQVAKASI
                     GEVQETAKSALAVKDAQELLSLQAGLLQPAAEKAAAYGRHLYDIAATTNAEVSRITEG
                     QLASAQAKFTSAVDGAVKNAPAGTESAAALVKSAIAAASNAMESVQKATKQAADVAEA
                     NFQAITNSAVKATQSTAAKAAGAAKRSTAA"
     gene            complement(1166..2149)
                     /locus_tag="VF453_19330"
     CDS             complement(1166..2149)
                     /locus_tag="VF453_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7689868.1"
                     /translation="MRLARILRAGAAVATAAACTLLAACGSTPISRPQPELTAQCATA
                     PDSVASAVAAAVTAPVAVPRPTRRPRVGLALGGGAARGFAHIGVIEVLEENGIHPDLI
                     AGTSAGSVVAALYAYGRSGRDLAQMADKMDESAITDWAFPGRGLIVGDALADFIRESV
                     HGKEIDQLRLPLGIVATDLDSGRSIVFRHGDTGTAVRASSSVPGVFRPVKIGSREYVD
                     GGLTEPVPVRAVRDMGADVVIAVDISQAPDGGDTSNTVRMLLQTFSIMGRSINELELK
                     EADVVVRPRLIGVSGTDFSLRQKNILAGREAALAVLPLIKAKLAQQVPLGR"
     gene            2277..3977
                     /locus_tag="VF453_19335"
     CDS             2277..3977
                     /locus_tag="VF453_19335"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX7689869.1"
                     /translation="MYQYTEFDRQFVRARAAQFRDQLERNLAGELSDDDFRPLRLQNG
                     WYIQRHAPMTRIAVPYGELSSRQLRQLARVARELDRGYLHFTTRHNAQFNWIPLPRAA
                     DAMDLLAEVDMHGIQTSGNCIRNITTDVFAGIAPDEQIDPRPYCEILRQWSTLQPEFA
                     FLPRKFKIAVSGSAEDRAAIAWHDIGLQLRRDESGAIGFKVLVGGGMGRTPVIASVAS
                     EFVPWNQILVYIEAIVRVYNRFGRRDNAFKARIKILVKAEGRKFFDEVEAEFRKILSR
                     DEGGAAHIIPQAEFDRVAAFFTRPERVHPAIGGFDPNRTGAAPVAFLRWLDRNVHPHQ
                     VEGYRAVTLSLKRPGQAPGDVTSEQMESAATMADRYSLGELRVSHEQNLLLPWVREAD
                     LRAVWHAAQEAGFATPNIGLLTDMIACPGGDVCALANARSIPIAAAITDQFDDLDALL
                     DLGEIDLHISGCMNSCGHHHTGHIGVLGVDKDGSEWYQVSVGGSDGTLVSGAPIPGKV
                     IGPSFSADEVPAVVQALIEVYRAHRDEDERFVDTARRLGVAPFRAAADAVRRETAVAA
                     "
     gene            4036..4515
                     /locus_tag="VF453_19340"
     CDS             4036..4515
                     /locus_tag="VF453_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEX7689870.1"
                     /translation="MKFIDRSTDAWQLLPDSEIAGPAPNRLLTLVQWQAVRDAWPADV
                     PVGVLLPNTADVEELAEDLPRLALVALEFPKWVDGRAYSQARVLRARLRFKGEIRATG
                     DVVVDMMPLLQRTGVDAVVLREGQQRSSAERALTFFPGGFYQGDVLQPQPLFAREPA"
     gene            4533..5351
                     /locus_tag="VF453_19345"
     CDS             4533..5351
                     /locus_tag="VF453_19345"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX7689871.1"
                     /translation="MNATTQAESSAVSPVDAVSSLSPIGAPATASAIALYAHPSADFA
                     AKLDATIEHLKAAAADHGGRIVQATSLGAEDMVVTDLIARLGLPIAIGTLETGKLHPQ
                     TVALIPRIRERYGIEVEVYRPREEQVVHFVRTNGEDAMYRSLELRKACCGVRKMEPLG
                     RMLEGRAAWVTGLRREQSSNRGAVAPFETEANGRTKVNPLADWSWNDVWHYIAAHEVP
                     YNPLHDEFMPSIGCAPCTRAIAVGEDFRAGRWWWEDEAAKECGLHATRKELAAA"
     gene            5499..>5862
                     /locus_tag="VF453_19350"
     CDS             5499..>5862
                     /locus_tag="VF453_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010102148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7689872.1"
                     /translation="MSTVTIVGAGPGSADLITVRGARALGRATVVLHDALVDPALREL
                     APAAEWIDVGKRGFCDSTAQTRINALLVQHARRDGARVVRLKGGDPSVFGRLDEELEA
                     LAQAGIVCEVVPGVTSAIA"
BASE COUNT          895 a         2211 c         1973 g          783 t
ORIGIN      
        1 gccggcgtcc agggcagccc gatctcgcgc tggcgggcgg cgtccagcgt cccgcccagc
       61 acgtcggcga tgtagcccgg gtcgtgcgcc agcgcgagct cgccttcgct ggcggccggg
      121 gcgggccgca ggacgatgcc gcccggctcg gcctggagcc gccggcgcag cgcgccgtac
      181 ttgccgaccg ggaagggatg cccttccggc aacgggagca tgtgcgcgtc gaagtggaaa
      241 gcgatcatgc aaaggtgtac aaagtcacgt tctggagcaa tgaccctaaa gtttgctgca
      301 gcgcagcaaa gctgcttgca atcgctgcgc cctcccctca tacttcggtt catgctgcgg
      361 tgcagcaacg actttccacc cccgagaccc gcaaaggaga actgtccatg ctgactgctg
      421 aacaactgct cgccgcccag aaggccaacc tggaaaccct gttcgagctg accaacaagg
      481 cgttcgaagg cgtcgagaag ctggtcgagc tgaacctgca ggtcgccaag gcgtccatcg
      541 gcgaagtcca ggaaaccgcg aagtcggccc tggcggtcaa ggatgcccag gaactcctgt
      601 cgctgcaagc cggcctgctc cagcccgccg ccgagaaggc cgccgcctac ggccgccacc
      661 tctacgacat cgccgcgacc accaacgccg aggtctcccg catcaccgaa ggccagctcg
      721 cctccgccca ggccaagttc acgagcgccg tcgacggtgc cgtgaagaac gccccggccg
      781 gcaccgagtc cgccgccgcc ctcgtgaagt cggccatcgc cgccgcctcc aacgcgatgg
      841 agagcgtcca gaaggccacc aagcaggccg ccgacgtcgc cgaagccaac ttccaggcga
      901 tcaccaacag cgccgtcaag gccacgcaga gcaccgccgc caaggctgcc ggcgccgcca
      961 agcgcagcac cgcggcctga tcccgcgaca ccgggccggg gacggcctgg cgcgcccggc
     1021 ttgaaaccgg cgcccgcgtc cccagctgct ggacatcggc cgcgcggccg atgcaaggtt
     1081 gtctcctcgg tacccccgcg gtaccttacg aacccggcgc cccaccgcgc cgggtttttc
     1141 ttttcttgcg acgccgagcg ccgcctcagc gtcccagcgg cacctgttgc gccagcttcg
     1201 ccttgatgag cggcagcacc gcgagcgccg cctcgcggcc ggcgaggatg ttcttctggc
     1261 gcaacgagaa gtcggtgccc gacaccccga tcagccgcgg tcggacgacg acgtcggcct
     1321 ccttcagctc gagctcgttg atgctgcgcc ccatgatcga gaaggtctgc agcagcatgc
     1381 gcaccgtgtt gctggtgtcg ccgccgtccg gcgcctgcga gatgtcgacc gcgatgacga
     1441 cgtccgcgcc catgtcgcgc acggcgcgca ccggcaccgg ctcggtgagc ccgccgtcga
     1501 cgtactcgcg gctgccgatc ttcaccggcc ggaacacgcc cggcaccgag ctggacgccc
     1561 ggacggccgt gccggtgtcg ccgtggcgga agacgatcga ccggccgctg tcgaggtcgg
     1621 tcgcgacgat gcccagcggc aggcgcagct ggtcgatctc cttgccgtgg acggactcgc
     1681 ggatgaagtc ggcgagcgca tcgccgacga tcaggccgcg gccgggaaag gcccagtcgg
     1741 tgatcgccga ctcgtccatc ttgtcggcca tctgcgccag gtcgcggccg ctgcggccgt
     1801 aggcgtagag ggccgcgacc acgctgccgg cggaggtgcc ggcgatcagg tcgggatgga
     1861 tgccgttctc ctccagcacc tcgatcacgc cgatgtgggc gaagccgcgc gccgcgccgc
     1921 cgcccagcgc cagcccgacg cgcggccggc gggtcgggcg cggcacggcc accggtgccg
     1981 tcacggccgc cgccacggcc gaggcgacgc tgtcgggcgc ggtcgcgcac tgggcggtca
     2041 gttcgggctg gggacgagag atcggggtgc tgccgcaagc ggccagcagc gtgcaggccg
     2101 cggccgtcgc gacggccgcg ccggcgcgca ggatcctcgc cagccgcatt tccatattcc
     2161 tcaaaggcat gattgcatag aaaaagtgta gttcccgtta tgaagacggc tcgctacatt
     2221 gtccactcat cagggaaaac gcggcatccc gcctcccgga aatcgagacc ccgacgatgt
     2281 accagtacac cgaattcgac cgccagttcg tccgcgcccg cgccgcgcag ttccgcgacc
     2341 agctggaacg caacctcgcc ggcgagctgt ccgacgacga ttttcgcccg ctgcgcctgc
     2401 agaacggctg gtacatccag cgccacgcac ccatgacgcg gatcgccgtg ccctacggcg
     2461 agctgtcgag ccggcagctg cgccagctcg cccgggtggc gcgcgagctc gaccgtggct
     2521 acctgcactt caccacgcgc cacaacgcgc agttcaactg gatcccgctg ccgcgcgccg
     2581 ccgacgcgat ggacctgctg gccgaggtgg acatgcacgg catccagact tcgggcaact
     2641 gcatccgcaa catcacgacg gacgtcttcg ccggcatcgc gccggacgag cagatcgacc
     2701 cgcggccgta ctgcgagatc ctgcgccagt ggagcacgct gcagcccgag ttcgccttcc
     2761 tgccgcgcaa gttcaagatc gcggtgagcg gctcggccga ggatcgcgcc gcgatcgcct
     2821 ggcacgacat cggcctccag ctgcgccgcg acgagtccgg ggcgatcggc ttcaaggtgc
     2881 tggtcggcgg cggcatgggc cgcacgccgg tgatcgcctc ggtggccagc gagttcgtgc
     2941 cctggaacca gatcctcgtc tacatcgagg ccatcgtgcg cgtctacaac cgcttcgggc
     3001 ggcgcgacaa cgcgttcaag gcgcgcatca agatcctcgt gaaggccgag ggccggaagt
     3061 tcttcgacga ggtcgaggcc gagttccgca agatcctctc ccgggacgaa ggcggcgccg
     3121 cgcacatcat cccgcaggcc gagttcgacc gcgtcgccgc gttcttcacg cgtcccgaac
     3181 gcgtgcaccc ggccatcggc ggcttcgacc cgaaccgcac cggcgccgcg ccggtggcct
     3241 tcctgcgctg gctcgaccgc aacgtgcacc cgcaccaggt cgagggctac cgtgccgtga
     3301 cgctgtcgct caagcgcccg ggccaggcgc ccggcgacgt cacgtccgag cagatggaat
     3361 ccgccgccac gatggccgac cgctactcgc tgggcgagct gcgcgtctcg cacgagcaga
     3421 acctgctgct gccctgggtg cgcgaggccg acttgcgcgc cgtctggcac gccgcgcagg
     3481 aagcgggctt cgcgacgccc aacatcggcc tgctgaccga catgatcgcg tgcccgggcg
     3541 gcgacgtctg cgcgctcgcc aacgcgcgct cgatcccgat cgccgcggcc atcaccgacc
     3601 agttcgacga cctggacgcg ctgctggacc tgggcgagat cgacctgcac atcagcggct
     3661 gcatgaactc ctgcggccac caccacaccg gccacatcgg cgtcctgggc gtcgacaagg
     3721 acggcagcga gtggtaccag gtgagcgtcg gcggctcgga cggcacgctc gtgagcggcg
     3781 ccccgatccc cggcaaggtc atcggcccgt cgttctcggc cgacgaagtg ccggccgtcg
     3841 tgcaggcgct gatcgaggtc taccgcgcgc accgcgacga ggacgagcgc ttcgtcgaca
     3901 ccgcccgccg cctcggcgtc gcgccgttcc gcgccgcggc cgacgccgtc cgccgcgaga
     3961 ccgccgtcgc cgcctgagcc cccgcccgcg aaccgtccga ccgatcgaat cccgtccccg
     4021 tctcctcgcc ccgtcatgaa gttcatcgac cgctccaccg acgcctggca gttgctgccg
     4081 gactccgaga tcgccggccc cgcgccgaac cgcctgctga cgctcgtgca gtggcaggcc
     4141 gtgcgcgatg cctggcccgc cgacgtgcct gtgggcgtgc tgctgcccaa cacggccgac
     4201 gtcgaggagc tcgccgagga cctgccgcgg ctggcgctcg tcgcgctgga gttcccgaag
     4261 tgggtcgacg gccgcgccta ctcgcaggcc cgcgtgctgc gcgcccgcct gcgcttcaag
     4321 ggcgagatcc gggccaccgg cgacgtcgtc gtcgacatga tgcccctgct gcagcgaacc
     4381 ggcgtcgacg ccgtcgtgct gcgggagggc cagcagcgct cgtccgccga gcgggcgctg
     4441 acgttcttcc cgggcggctt ctaccagggc gacgtgctgc agccgcaacc gctgttcgcg
     4501 cgcgaaccgg cctgacgaaa ggggacgacg ccatgaacgc caccacgcag gccgagagct
     4561 cggccgtctc gcccgtcgac gcggtctcgt cgctgagccc gatcggcgcg cccgcgacgg
     4621 cctcggcgat cgcgttgtac gcgcatccga gcgccgactt cgccgccaag ctcgacgcca
     4681 ccatcgagca cctgaaggcg gccgcggccg accacggcgg ccgcatcgtc caggccacca
     4741 gcctcggcgc cgaggacatg gtcgtcaccg acctgatcgc ccgcctcggg ctgccgatcg
     4801 ccatcggcac gctcgagacc ggcaagctgc atccgcagac ggtcgcgttg atcccgcgca
     4861 tccgcgagcg ctacggcatc gaggtcgagg tctaccgccc gcgcgaggag caggtcgtcc
     4921 acttcgtgcg cacgaacggc gaggacgcga tgtaccgcag cctcgagctg cgcaaggcct
     4981 gctgcggcgt gcgcaagatg gagccgctcg gccgcatgct cgagggccgc gccgcctggg
     5041 tgaccgggct gcgccgcgag cagtcgagca accgcggcgc cgtcgcgccg ttcgagaccg
     5101 aggccaacgg ccgcaccaag gtgaacccgc tcgccgactg gtcctggaac gacgtgtggc
     5161 actacatcgc cgcgcacgag gtgccctaca acccgctgca cgacgagttc atgcccagca
     5221 tcggctgcgc accctgcacg cgcgcgatcg ccgtcggcga ggacttccgc gccggccgct
     5281 ggtggtggga ggacgaggcc gccaaggaat gcgggctgca cgcgacgcgc aaggagctcg
     5341 cggccgcctg agcgtcgcga cggacaagcc gccgccggcc agccgccgcg cccagggcgc
     5401 ggataggcca cgacgggcgc atcatcgccc cgtgccgcga acgggccccg atcctgcttg
     5461 aacgcggacg tccgccactt cctgcgagcc cgaccatcat gagcaccgtc accatcgtgg
     5521 gcgccggccc cggatccgcc gacctcatca ccgtgcgcgg ggcgcgcgcg ctcggccgcg
     5581 ccacggtggt gctgcacgac gcgctcgtcg atcccgcgct gcgcgagctc gcgcccgcgg
     5641 ccgagtggat cgacgtgggc aagcgcggct tctgcgactc gacggcgcag acccgcatca
     5701 acgccctgct ggtgcagcac gcgcgccgcg atggtgcgcg cgtcgtgcgg ctcaagggcg
     5761 gcgacccgag cgtgtttggg cgcctcgacg aggagctgga ggcgctcgcc caggccggca
     5821 tcgtctgcga ggtcgtgccc ggcgtcacct cggccatcgc cg
//
LOCUS       DASVLN010000816         5399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_203155, whole genome shotgun sequence.
ACCESSION   DASVLN010000816 DASVLN010000000
VERSION     DASVLN010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203155"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VF453_19355"
     CDS             <1..326
                     /locus_tag="VF453_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689873.1"
                     /translation="QAALEAAGIAVSVVHDGRAALVEAHAREFDAAILDIGMPGLNGY
                     DLAGALRADAKTARLVLLAVSGWGQADDKERAVAAGFDRHFVKPVPPDVLIAALGEAL
                     RVERP"
     gene            344..2233
                     /locus_tag="VF453_19360"
     CDS             344..2233
                     /locus_tag="VF453_19360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7689874.1"
                     /translation="MTRPPCLGAARALALAALLPLAAATAQAAILSSADLGDLSIEDL
                     GRIPVTSVSGRPEAVQDAAASIFVITADDIRRSAATSLPEALRLAPNLQVARINATQY
                     AISARGFNNAIGNKLLVMVDGRTVYSPLFSGVFWDAQQVPLADVERIEVISGAGGTLW
                     GANAVNGVINVITRAAQDTPGWLLAAHAGATGNEATLRYGGALPGGGWRAYAMQSDRA
                     STTLSSGKAVDDHQDRQQGGFRADWSSRADSYTVQGDVYQSAGDGRTPTSADMSGANL
                     LARWRHGEAGGGNWQLQAWFDEASRDDQLEFHDHVRTLDLQFNHAPALGADHRLIWGL
                     GYRQAIDQTDPALIVGFDPRTRLLHWANVFAQDDVRVTQRLRVIGGAKVETNAYTGAE
                     FLPTLRAAWDLGAAGTLWGSASRAVRAPARLDRDVQIPAKPPFIIDRGPDFHSEVADV
                     FEIGLRGQRASGFGYSATVFHHHYRDLRAGRAAPTTVDNLAWGDVSGLELWGNADVTR
                     DWRLALGWTELRESLRAAPTAGLDSVSNLGDDPRRQIQLRSTMRPVRDVDLDLTLRHV
                     AALPAPAVPAYTVVDARVAWSVRPDLDLSLIAQELGRRHVEFDPASSSRFGPRAFVQL
                     EWRTP"
     gene            2308..3690
                     /locus_tag="VF453_19365"
     CDS             2308..3690
                     /locus_tag="VF453_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7689875.1"
                     /translation="MTRRADTAARSRYKDKMHRDKPRLSFWQILNMNLGFFGIQFSFG
                     LQQSNMSPIYKYLGADEASLPLLWLAGPITGLVVQPLIGALSDRTTSRWGRRTPYFLA
                     GALLCSLGLLAMPFSPALWFAAGLLWILDTANNVAMEPYRAYVSDRLRPEQHGLGYLT
                     QSAFTGLAQTLAYLVPSLLVWIGMDPDATNGHHIPQVTIVAFLVGAVFSFTTVWWSVR
                     STPELPLTAQEERVLRARERGAAAAVSDIVAAVREMPPVMRRLWWMKLFQWYGMLCYW
                     IYIVPALASTMFGRPAADSAGFRAAGLLAGQLGGFYNFVAFLAAFAMVPLAARWGARR
                     VHATCLALAGLGMCALPALHSIGLLFLPMVGIGLAWASMMGNPYILLAGAIPPERAGV
                     YMGIFNMFIVLPMILQMLTMPLYFHAWLGGEPVNAIRVAGSCLLAAAAATCFVRAPAG
                     ARPVASPVDA"
     gene            3710..5218
                     /gene="gtfA"
                     /locus_tag="VF453_19370"
     CDS             3710..5218
                     /gene="gtfA"
                     /locus_tag="VF453_19370"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEX7689876.1"
                     /translation="MQNQVQLITYVDRLSGGGFKELRALLDGPLAGVFGGVHLLPFFD
                     PIDGADAGFDPIDHTRVDARLGSWDDVRAIAQRLDVMADVIVNHISSESPQFLDYARH
                     GAASPHDGLFLTFDRVFPDGADEADLLRIYRPRPGLPLREAMVGGQRRILWSTFTPQQ
                     LDIDVTHPAGRAYLDGILQRLAENGVRMVRMDAVGYAIKKAGTDCFMTPETFAFIEDF
                     SDRARALGMEVLVEVHSHYSRQIEIADRVDWVYDFALPPLLLHAFAFGTADALRHWID
                     IRPNNALTVLDTHDGIGIIDIGADAADREQRPGLVPPKELDGLVERIHAASRGESRLA
                     TGAAASNLDLYQVNCTFHAALGGDDLTYLLARAVQFFLPGIPQVYYVGLLAGGNDMAL
                     LRETGIGRDINRRRFAPGEAEAALARPVVRELLRLIRLRNESPAFDGCFRSRTVAEHG
                     LALEWTNGPHAARLSVDLRSRAYAIDLVDDGAASSFRFDLDASGSAPPAAPL"
BASE COUNT          744 a         2035 c         1827 g          793 t
ORIGIN      
        1 tgcaggccgc gctcgaggcg gccggcatcg cggtgagcgt cgtgcacgac ggacgcgcag
       61 cgctcgtcga ggcgcatgcg cgcgagttcg acgccgcgat cctcgacatc ggcatgcccg
      121 ggctgaacgg ctacgacctc gccggcgcgc tgcgcgccga cgcgaagacg gcgcgcctcg
      181 tcctgctcgc ggtcagcggc tggggccagg ccgacgacaa ggagcgcgcg gtggcggccg
      241 gcttcgaccg ccacttcgtg aagccggtac cgcccgacgt attgatcgcg gcgctcggag
      301 aagcgctgcg cgtggagcga ccatgacaag aaccccgcca gacatgaccc gccctccctg
      361 cctcggcgcc gcgcgcgcgc tcgcgctcgc ggcgctgctg ccgctcgcag ccgcgaccgc
      421 gcaggccgcc atcctgtcgt cggccgacct cggcgacctg agcatcgagg acctcggccg
      481 catcccggtg acgtcggtga gcggccgccc cgaggcggtg caggacgcag ccgcatcgat
      541 cttcgtcatc accgccgacg acatccgccg ttcggccgcg accagcctgc ccgaagcgtt
      601 gcgcctcgca cccaacctgc aggtggcccg catcaacgcc acccagtacg ccatcagcgc
      661 gcgcggcttc aacaacgcga tcggcaacaa gctgctggtg atggtcgacg gccgcacggt
      721 ctactcgcca ttgttctccg gcgtgttctg ggacgcgcag caggtgccgc tcgccgacgt
      781 cgagcgcatc gaggtgatca gcggcgcggg cggcacgctg tggggcgcca acgcggtcaa
      841 cggcgtgatc aacgtcatca cgcgagccgc gcaggacacc cccggctggc tgctcgccgc
      901 gcatgccggg gcgaccggca acgaggccac cctgcgctac ggcggcgccc ttcccggcgg
      961 cggctggcgg gcatacgcga tgcagtccga ccgcgcgagc accacgctgt cctcgggcaa
     1021 ggccgtcgac gaccaccagg accgccagca gggcggcttc cgggcggact ggagctcgcg
     1081 cgcggattcg tacaccgtcc agggtgacgt ctaccagtcc gcgggcgacg ggcgcacccc
     1141 gacctcggcc gacatgtcgg gcgccaacct gctcgcgcgc tggcgccacg gcgaggcagg
     1201 cggcggcaac tggcagttgc aggcgtggtt cgacgaggcg agccgcgacg accagctcga
     1261 gttccacgac cacgtgcgca cgctggacct gcagttcaac cacgcgccgg ccctcggcgc
     1321 cgatcatcgc ctgatctggg gcctcggcta ccggcaggcg atcgaccaga ccgatcccgc
     1381 gctgatcgtc ggcttcgacc cgcgcacgcg cctgctgcat tgggccaacg tcttcgcgca
     1441 ggacgacgtc cgcgtgacgc agcgcctgcg cgtgatcggc ggcgccaagg tcgagaccaa
     1501 cgcctacacc ggcgccgagt tcctgccgac gctgcgcgcc gcgtgggatc tcggcgcggc
     1561 tggcacgctg tggggctcgg cctcgcgcgc ggtgcgggcg ccggcgcggc tcgaccgcga
     1621 cgtgcagatc ccggccaagc cgcccttcat catcgaccgc ggcccggatt tccactccga
     1681 ggtcgccgac gtgttcgaga tcggcctgcg cggccagcgc gcctccggct tcggctattc
     1741 cgcgaccgtc ttccatcacc actaccgcga cctgcgcgcc ggacgcgccg cgcccacgac
     1801 ggtcgacaac ctcgcctggg gcgacgtcag cgggctcgag ctgtggggca atgccgacgt
     1861 cacgcgcgac tggcgcctgg cgctcgggtg gacggagctg cgcgagtcgc tgcgcgcggc
     1921 gccgacggcg gggctcgact cggtgagcaa ccttggcgac gatccgcgcc gccagatcca
     1981 gctgcgctcg acgatgcggc cggtccgcga cgtcgacctc gacctgacgc tgcgccacgt
     2041 cgccgcgctg ccggcgccgg ccgtgccggc ctacacggtg gtcgacgcgc gcgtggcatg
     2101 gtcggtgcga ccggacctcg acctgagcct gatcgcgcag gagctgggcc gccgccacgt
     2161 cgagttcgac cccgcgagtt ccagccgctt cgggccgcgg gcgttcgtgc agctggagtg
     2221 gcgcacgccc tgaccgcatg cgcgacgcat cgcgcgtccg cgttttccct ggtatgcaat
     2281 cgattgcatc acttggacac tggcgacatg acgcgacgtg cggacaccgc cgcgcgcagc
     2341 cggtacaagg acaagatgca tcgagacaag ccccgcctgt cgttctggca gatcctgaac
     2401 atgaacctgg gattcttcgg aatccagttc agcttcgggc tgcaacagag caacatgagc
     2461 ccgatctaca agtacctcgg cgcggacgag gccagcctgc cgctgctgtg gctcgccggg
     2521 ccgatcacgg ggctcgtggt gcagccgctg atcggcgcgc tcagcgaccg cacgaccagc
     2581 cgctgggggc ggcgcacgcc ctacttcctc gccggcgcgc tgctgtgcag cctgggcctg
     2641 ctcgcgatgc cgttcagccc ggcgctgtgg ttcgcggccg gcctgctgtg gatcctggac
     2701 accgccaaca acgtggccat ggagccgtac cgtgcgtacg tgagcgaccg gctgcggccg
     2761 gagcagcacg gcctgggcta cctgacccag agcgccttca cggggctcgc gcagacgctg
     2821 gcctatctgg tgcccagcct gctcgtgtgg atcggcatgg atccggacgc gaccaacggc
     2881 caccacatcc cgcaggtcac gatcgtcgcc ttcctggtcg gggcggtgtt ctcgttcacg
     2941 acggtgtggt ggtcggtgcg ctcgacgccc gagttgccgc tcaccgcgca ggaggaacgc
     3001 gtcctgcgcg cccgcgagcg cggcgcggcg gccgccgtgt ccgacatcgt cgccgccgtg
     3061 cgggagatgc cgcccgtcat gcgccggctg tggtggatga agctcttcca gtggtacggc
     3121 atgctgtgct actggatcta catcgtgccg gcgctcgcgt cgacgatgtt cggccggccg
     3181 gccgccgatt cggccggctt ccgcgccgcg ggactgctgg cggggcaact cggaggcttc
     3241 tacaacttcg tggccttcct cgcggcgttc gcgatggtgc cgctggcggc acgctggggc
     3301 gcgcgccgcg tgcacgcgac ctgcctcgcg ctggccggcc tgggcatgtg cgcgctgccg
     3361 gccctccatt cgatcggcct gctgttcctg ccgatggtgg gcatcggcct cgcctgggcc
     3421 agcatgatgg gcaatcccta catccttctc gcgggcgcga tcccgccgga gcgcgcaggc
     3481 gtctacatgg gcatcttcaa catgttcatc gtgctcccga tgatcctgca gatgctcacg
     3541 atgccgctgt acttccatgc gtggctgggc ggcgagcccg tcaacgcgat ccgcgtcgcg
     3601 gggagctgcc tgctggcggc cgccgcggcc acctgcttcg tgcgcgcgcc ggccggcgcg
     3661 cggcccgtcg cctcacccgt ggacgcctga accgtccgca cgtcctccca tgcagaacca
     3721 ggtccagctc atcacctacg tcgaccgcct gtccggcggc ggattcaagg aactgcgcgc
     3781 gctgctcgac ggcccgctcg cgggcgtctt cggcggcgtc catctcctgc ccttcttcga
     3841 cccgatcgac ggcgccgacg ccggcttcga cccgatcgac cacacgcgcg tcgacgctcg
     3901 cctcggcagc tgggacgacg tgcgcgcgat cgcgcagcgg ctggacgtga tggccgacgt
     3961 gatcgtgaac cacatctcga gcgaatcgcc gcagttcctg gactacgccc ggcacggcgc
     4021 cgcctcgccg cacgatggcc tgttcctcac cttcgaccgc gtgttccccg acggcgccga
     4081 cgaagccgac ctgctgcgca tctaccgccc gcggcccggg ctgccgctgc gcgaggcgat
     4141 ggtcggcgga cagcgccgca tcctgtggtc gacgttcacg ccgcagcagc tcgacatcga
     4201 cgtgacgcac ccggccggcc gcgcctacct ggacggcatc ctgcagcgcc tggccgaaaa
     4261 tggcgtgcgc atggtgcgca tggacgccgt gggctacgcg atcaagaagg ccggcaccga
     4321 ctgcttcatg acgcccgaga ccttcgcctt catcgaagac ttctcggatc gcgcgcgcgc
     4381 gctcggcatg gaggtgctgg tcgaggtgca ttcgcactac tcgcgccaga tcgagatcgc
     4441 cgaccgcgtc gactgggtct acgacttcgc gctgccgcca ctgctgctgc acgcgttcgc
     4501 gttcggcacc gccgacgcgc tgcggcactg gatcgacatc cggcccaaca acgcgctgac
     4561 cgtgctcgac acccacgacg gtatcggcat catcgacatc ggcgccgatg cggcagaccg
     4621 cgaacagcgg ccagggctgg tgccgccgaa ggagctcgac ggcctcgtcg aacgcatcca
     4681 cgccgcgagc cgcggggaga gccggctggc gaccggcgcc gcggcatcga acctcgacct
     4741 ctaccaggtc aactgcacct tccacgccgc gctcggcggc gacgacctca cctacctgct
     4801 cgcgcgcgcg gtgcagttct tcctgcccgg catcccgcag gtctattacg tcggcctgct
     4861 cgccggcggc aacgacatgg ccctgctgcg cgagacgggc atcggccgcg acatcaaccg
     4921 ccgccgcttc gcgccgggcg aggccgaggc ggcgctcgcg cggcccgtcg tgcgcgaact
     4981 gctgcgcctg atccggctgc gcaacgagtc gccggcgttc gacggctgct tccgatcgcg
     5041 gacggtcgcg gagcacgggc tcgcgctcga atggacgaac ggcccccatg ccgcgcgcct
     5101 gtcggtggac ctgcgcagcc gcgcctacgc gatcgacctg gtcgacgacg gcgcggcgag
     5161 cagcttccgc ttcgacctcg atgcgagcgg atcggcaccg cccgccgcgc ctctttgatc
     5221 gccccttcat gcaatcgatt gcagcttgat tcgacaacca gaggcgccca gcgcccacga
     5281 cccgaccggg cgtcccgcgc ccgcatgacg tacaacctgg agacatcgat gaaccgccca
     5341 cgccttccga agaccccgct cgccctggcc gccgccctgg cgctgcccgc cctgcccgc
//
LOCUS       DASVLN010000817         2788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_203196, whole genome shotgun sequence.
ACCESSION   DASVLN010000817 DASVLN010000000
VERSION     DASVLN010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203196"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..529
                     /gene="rlmH"
                     /locus_tag="VF453_19375"
     CDS             59..529
                     /gene="rlmH"
                     /locus_tag="VF453_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX7689877.1"
                     /translation="MRLIVAAVGQKPPAWAETAYEEFERRFPPEMRLELKAVKAEPRT
                     AGKAAAQMMQAEGKRLEAVLPPGARRVVLDEHGTRWTTRALAERVAFWRGDGRDVAFL
                     IGGPDGLDPALKATADETARLSDLTLPHAFVRVLLAEALYRAWSLSTGHPYHRE"
     gene            537..1163
                     /locus_tag="VF453_19380"
     CDS             537..1163
                     /locus_tag="VF453_19380"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX7689878.1"
                     /translation="MARASHDFVYLASQSPRRRQLLDQLGVRHEPLLPAPDEDAEALE
                     AQLPGEKPADYCVRVTQLKLDAALARRKARGLPPAPVLCADTTVALGARIFGKPIDAA
                     DAQATLARLSGRTHRVMTAVAVASASGRVRDARLSVSTVRFASMPADVIAHYVASGEP
                     FGKAGAYAIQSRIAGWIERIAGSYSGIMGLPLHETAQLLLAAGVALQR"
     gene            1188..2651
                     /gene="rng"
                     /locus_tag="VF453_19385"
     CDS             1188..2651
                     /gene="rng"
                     /locus_tag="VF453_19385"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEX7689879.1"
                     /translation="MHDILVNWSPQETRVAIIENGAVQELHIERALERGLVGNVYLGK
                     VARVLPGMQSAFIDIGLERAAFLHVADLHGHPAAKPEGMPAAIEKLVFEGQSLTVQVV
                     KDPIGTKGARLTTQVSIAGRLLVYLPHDNHIGISQRIGSHETREALRARMIALAGNPA
                     EGGGGFILRTNAEEASDQELADDIAYLRKTWAQIRQRAMSRPAGSLLHQDLSLAERVL
                     RDIASEGTQSIRIDSKTQYESLRAFGDNYAPGSVAKLEHYRGERPIFDLYNVDEEIAR
                     ALARRVDLKSGGYLIIDQTEALTTIDVNTGGFVGARNFDETIFKTNLEAAGAISRQLR
                     LRNLGGIIIVDFIDMAREEHQAAVLTEFRKQLSKDRTKVTVSGFTQLGLVEMTRKRTR
                     ESLAHMLCDPCPTCAGRGQVKTARSVCYDVLREILREARQFNPKEFRVIASASVVEML
                     LDEESAHLADLSAFIRKPISLSAEPTMNPEQYDIVLL"
BASE COUNT          426 a         1052 c          959 g          351 t
ORIGIN      
        1 cggcttcgtg cgcgtcgcgc ccgcgtgacg cgcgcgcggg cgagcgcggg ggcgcgccgt
       61 gcgcctgatc gtcgccgccg tcggtcagaa gccccccgcc tgggccgaga cggcgtacga
      121 ggaattcgag cgtcgcttcc cgcccgagat gcggctggag ctgaaggccg tgaaggccga
      181 gccgcgcacc gccggcaagg ccgccgcgca gatgatgcag gcggaaggca agcggctgga
      241 ggccgtgctg ccccccggcg cgcgccgcgt cgtcctcgac gagcacggca cgcgctggac
      301 gacccgggcg ctcgcggagc gcgtcgcgtt ctggcgcggc gacggccgcg acgtcgcctt
      361 cctcatcggc ggccccgacg gcctcgatcc cgcgctgaag gccaccgccg acgagaccgc
      421 gcgcctgtcc gacctcacgc tgccgcacgc cttcgtgcgc gtgctgctgg ccgaggcgct
      481 gtaccgggcc tggtcgctgt cgaccggcca tccctaccac cgcgagtgag cgcccgatgg
      541 cccgcgcgtc ccacgacttc gtctacctcg cctcgcagtc gccgcgccgc aggcaactgc
      601 tcgaccagct cggcgtgcgc cacgagccgc tgctgcccgc gccggacgag gacgccgagg
      661 ccctcgaggc gcagctgcct ggcgagaagc cggccgacta ctgcgtgcgc gtcacgcagc
      721 tgaagctcga cgccgcgctc gcgcgccgca aggcccgcgg cctgccgccc gcgccggtgc
      781 tgtgcgccga cacgaccgtc gcgctgggcg cgcgcatctt cggcaagccc atcgacgcgg
      841 ccgacgcgca ggccacgctc gcgcgcctgt cgggacgcac gcatcgcgtg atgacggcgg
      901 tcgccgtggc ctcggcttcg ggtcgcgtgc gcgatgcgcg gctcagcgtg tcgacggtgc
      961 gcttcgcgtc catgccggcc gacgtcatcg cgcactatgt cgcgagcggc gagcccttcg
     1021 gcaaggccgg cgcctacgcg atccagagcc gcatcgccgg ctggatcgag cgcatcgccg
     1081 gcagctactc cggtatcatg ggcctgccgc tccacgagac cgcgcagctg ctgctcgccg
     1141 cgggcgtcgc actccaacga tagcaagaga caacgggcgc cgccgccatg cacgacatcc
     1201 tggtcaactg gtccccgcag gagacccgcg tcgcgatcat cgagaacggc gccgtccagg
     1261 agctgcacat cgagcgcgcg ctcgaacgcg gcctcgtcgg caacgtctac ctgggcaagg
     1321 tcgcacgggt gctgccgggg atgcagtccg ccttcatcga catcggcctc gagcgcgccg
     1381 ccttcctgca cgtcgccgac ctccatggcc atcccgcggc caagcccgag ggcatgccgg
     1441 cggcgatcga gaagctggtg ttcgagggcc agtcgctgac ggtgcaggtg gtcaaggacc
     1501 cgatcggcac caagggcgcg cgcctgacca cgcaggtgag catcgcgggc cggctgctgg
     1561 tctacctgcc gcacgacaac cacatcggca tctcgcagcg catcggctcg cacgagacgc
     1621 gcgaggcgct gcgtgcgcgc atgatcgcgc tggccggcaa cccggccgag ggcggcggcg
     1681 gcttcatcct gcgcaccaac gcggaagagg cgagcgacca ggagctcgcc gacgacatcg
     1741 cctacctgcg caagacctgg gcgcagatcc gccagcgcgc gatgtcgcgg cccgcgggct
     1801 cgctgctgca ccaggacctg agcctggccg aacgcgtgct gcgcgacatc gcgagcgagg
     1861 gcacgcagtc catccgcatc gactccaaga cccagtacga gtcgctgcgg gccttcggcg
     1921 acaactacgc gccgggctcg gtggccaagc tggagcacta ccgcggcgag cggccgatct
     1981 tcgacctcta caacgtcgac gaggagatcg cgcgcgcgct cgcccgccgc gtcgacctga
     2041 agagcggcgg ctacctcatc atcgaccaga ccgaggccct gacgacgatc gacgtcaaca
     2101 ccggcggctt cgtcggcgcg cgcaacttcg acgagacgat cttcaagacc aacctggagg
     2161 cggcaggcgc gatcagccgc cagctgcgac tgcgcaacct gggcggcatc atcatcgtcg
     2221 acttcatcga catggcgcgc gaggagcatc aggccgccgt gctcaccgag ttccgcaagc
     2281 agctcagcaa ggatcgcacc aaggtcaccg tcagcggctt cacgcagctc gggctcgtcg
     2341 agatgacgcg caagcgcacc cgcgagtcgc tcgcccacat gctgtgcgac ccgtgcccga
     2401 cctgcgccgg ccgcgggcag gtcaagacgg cgcgcagcgt ctgctacgac gtgctgcgcg
     2461 agatcctgcg cgaggcgcgc cagttcaatc cgaaggagtt ccgcgtcatc gcgagcgcct
     2521 cggtcgtcga gatgctgctc gacgaggaaa gcgcccatct cgccgacctc tcggcgttca
     2581 tccgcaagcc gatctcgctg agcgccgagc cgacgatgaa cccggagcag tacgacatcg
     2641 tgctgctctg aggggccgcg ccagctcagc cgagcccgac cccgcgcgcc atcgccgccg
     2701 ccagcagcgg gatcggcacg aacagcgcca gctcggcgag cagccagcgg cgcgtggcgg
     2761 cgacctcggc ggccgtcggc gtgaagcc
//
LOCUS       DASVLN010000818         6816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_41197, whole genome shotgun sequence.
ACCESSION   DASVLN010000818 DASVLN010000000
VERSION     DASVLN010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41197"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF453_19390"
     CDS             complement(<1..279)
                     /locus_tag="VF453_19390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX7689880.1"
                     /translation="MTDVAFHTGIADPLGYACRLLRKAYRAGARVAVHGEAPALDRLD
                     QALWTFEPLEFVPHVLLPRDGADGGRLARTPIVLVKDGASAPAGCRVAV"
     gene            complement(329..1786)
                     /locus_tag="VF453_19395"
     CDS             complement(329..1786)
                     /locus_tag="VF453_19395"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX7689881.1"
                     /translation="MDFQTLTQPDAGVAGVNADALVVVLPAAGDLPSFGSVVDEAIAD
                     AVKGGDLERKAGKTLYLPRAAGVKAGRLVLAVARDGSAKAFKAAAAAALGALKSGPSK
                     HVAVAGGGATAAHAEALAIAAGDAVYHYAKTKPSAPPAPALAKVSYVVPKASAAAAKA
                     GLATGAAIVSGMDLARDCGNLPGNVATPTYLGEQALAMAKTHGLEVEVLDRRQIEKLG
                     MGSFLSVARGSDEPPRFIVMQWKGGGRKDAPVVLVGKGITFDTGGISLKPGAEMDEMK
                     FDMCGAASVLGTMRAVAELKAKVNVVGLIPTCDNMPSGKATKPGDVVTSMSGQTIEIL
                     NTDAEGRLILCDALTYAERFKPAAVVDIATLTGAIIIALGHHNSGLYSPDDGLAGELL
                     AASAAGLDPAWRMPLDEEYDETLKSNFADIPNIGGGRAGGSITAAKFLQRFTSKYPWA
                     HLDIAGTAHRSGPNKGATGRPVPLLTHFVLGRAGK"
     gene            1822..2928
                     /gene="lptF"
                     /locus_tag="VF453_19400"
     CDS             1822..2928
                     /gene="lptF"
                     /locus_tag="VF453_19400"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX7689882.1"
                     /translation="MLFDSTVRKELARSFGATLMVILTIFMTNLVIRTLGLAANGAVA
                     PQDVVLIFTLLGLQTLPTILALSLFVGVIISLGRMYRESEMAIWFSSGIGLKRFVRPV
                     LATSWPVIVVIAGLMLFVWPWSYGQYNDLRQRFEQRADVLRASPGQFQTSGDGKRVFF
                     VEKAGSAQAGGGRNVFIVNDLGGKESVAVSQSGKLQSENGERVLVLDSGHMQQTDKAT
                     GEHTHVSFDHYRVRVGETSGASNGTPSPRQIDTSELLLTGGNANMAELTWRIGLILGA
                     TNLLFIGISLSATNPRRASNWSLLFALLAFVIYYNLLNLSQSWVAAGRLPAAVALVGL
                     HGLAFGVGMGLIWWREYSTVVHPLRLILGRGAPR"
     gene            2925..4082
                     /gene="lptG"
                     /locus_tag="VF453_19405"
     CDS             2925..4082
                     /gene="lptG"
                     /locus_tag="VF453_19405"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX7689883.1"
                     /translation="MKTVRQLLYRDVFWSVFFVTVAFVALFFFIDFVDEMSRVGHGGY
                     TAKAAALATLLEQPGHVYELFPIAVLIGTIYSMARLAQSSEFTILRTAGLGPGRALSL
                     LGVIGLLFAVLTFGLGEYVTPHSEQAAAFLKARFGMGLAVGRTGAWLKEHRTDPDGER
                     SVTVNVRGVGKNGELTGVRIWEMNDAGREVRGITAEHAVVGRGRTVREWTLENATQTL
                     WDTGAATSAAAPASAASGATAPTVRVTHLDQMSWPTRLNASVAAAAVLPINSMGALDL
                     WRYSTHLAAQEQASQTYRMQFWKRVLYPFACLVMVALALPFAYLHARAGGVSVKVFGG
                     IMLGISFVLLNNVVGHLGQLHDWTPWIAASVPSVVYLLLSMSAFAWLVRYR"
     gene            4082..4495
                     /locus_tag="VF453_19410"
     CDS             4082..4495
                     /locus_tag="VF453_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEX7689884.1"
                     /translation="MTRAIVLLAHGARDPSWATPFETVAARVREKAPLTQVRLAFLEL
                     MSPDLATAGDELAAAGCAHVDVVPVFLGGGGHVRRDVPVQLDALRARHPQVAWRLHPA
                     LGETPHVIAALADAAVDLAGLAFAVPHDPQDGVGT"
     gene            4492..5436
                     /locus_tag="VF453_19415"
     CDS             4492..5436
                     /locus_tag="VF453_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEX7689885.1"
                     /translation="MNLHQFRFVQEAVRRNLNLTETAKALFTSQPGISKAILELEEEL
                     GVDLFVRHGKRLRRVTEPGREVLKAIDVILREVANMKRIGEEYSKQDAGTLSIAATHT
                     QARYVLPEPVAQLRRRFPKVQVSLHQGSPAEVAQMLLDGTADIGLATESLAEPEDLVT
                     LPCYEWEHVIVMRADDPLAQADKISLEQLAEQTLISYHPSFTGRRRIDAAFAQRRLKP
                     NIALEAIDSDVIKTYVRAGLGVGIVAEMAVRDDPPGGDLVHKPAGVLFGRNVTRVAFR
                     RGAYLRGFVLTFAELLSDRITRPLLLKALGGEGENYEL"
     gene            5426..6631
                     /locus_tag="VF453_19420"
     CDS             5426..6631
                     /locus_tag="VF453_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7689886.1"
                     /translation="MNSDRRASTAESLLARGAEAGQAVPSRLPAVGTTIFTVMSALAA
                     RTGAINLGQGFPDFDCDPALVDAVAAAMRAGHNQYPPMIGVPALREAIAAKVEATTGR
                     RYDPEAEITVTAGATQGLLSTILALVHPGDEVVVLEPCYDSYGPSIELAGGRIVRVAL
                     TPGTWRPDFDALAAALTPRTRLVIVNTPHNPSGTVWTQDERARLAEVLRPTRALVLSD
                     EVYEHMVFDGRAHASVSGDPELAERSVVVSSFGKTFHVTGWKVGYVAAPAALMREFRK
                     VHQFNVFTVNSAMQHGLAAYLADPGPWQGLAAFYQAKRDFFRAGLAPTRFRLLACEGS
                     YFQCVDYSAISDEPDAAFCERLTTGIGVAAIPMSAFYEAAPDMHVIRFCFAKREETLA
                     AALERLQRL"
BASE COUNT          923 a         2488 c         2361 g         1044 t
ORIGIN      
        1 gaccgcgacc cggcagcccg ccggcgcgct cgccccgtcc ttcaccagca cgatcggcgt
       61 gcgcgcgagc cggccgccgt cggcgccatc gcgcggcagg agcacgtggg gcacgaactc
      121 gagcggctcg aaggtccaca gcgcctggtc gaggcggtcg agcgccggcg cttcgccgtg
      181 cacggccacg cgtgcgcccg cgcggtaggc cttgcgcagg aggcggcagg cgtagccgag
      241 cgggtcggcg atgccggtgt ggaaggcgac gtcggtcacg cgcgttcggt cgtcgggcga
      301 gggagccggg cgccgggccc ggcccgcttc acttgcccgc gcgtcccagc acgaagtggg
      361 tgagcagcgg caccgggcgg cccgtcgcgc ccttgttcgg gcccgagcgg tgcgcggtgc
      421 cggcgatgtc caggtgcgcc cacgggtact tggaggtgaa gcgctgcagg aacttcgccg
      481 cggtgatcga gccccccgcg cggccgccgc cgatgttggg gatgtcggcg aaattgctct
      541 tgagcgtctc gtcgtactcc tcgtccagcg gcatgcgcca ggccggatcg aggccggccg
      601 cggacgccgc cagcagctcg cccgcgaggc cgtcgtcggg cgagtacagc ccgctgttgt
      661 gatgccccag cgcaatgatg atggcgcccg tgagcgtggc gatgtccacc accgcggccg
      721 gcttgaagcg ctcggcatag gtgagcgcgt cgcacaggat caggcggcct tccgcgtcgg
      781 tgttgaggat ctcgatcgtc tggcccgaca tgctcgtgac cacgtcgccc ggcttggtcg
      841 ccttgcccga aggcatgttg tcgcaggtcg ggatcaggcc gacgacgttg accttggcct
      901 tgagctcggc gaccgcgcgc atcgtgccca gcacgctcgc cgcgccgcac atgtcgaact
      961 tcatctcgtc catctcggca cccggcttca gcgagatgcc gccggtgtcg aaggtgatgc
     1021 ccttgccgac cagcacgacg ggcgcgtcct tgcggccgcc gcccttccac tgcatcacga
     1081 tgaagcgcgg tggctcgtcg gagccgcgcg ccacggacag gaacgagccc atgcccagct
     1141 tctcgatctg cctgcgatcg agcacctcga cttccagccc gtgcgtcttg gccatcgcca
     1201 gcgcctgctc gccgaggtag gtcggcgtcg ccacgttgcc cggcaggttg ccgcagtcgc
     1261 gcgcgagatc catgcccgag acgatggccg cccccgtcgc gaggccggcc ttggccgccg
     1321 cggcggacgc cttgggaacg acgtacgaca ccttggccag agccggcgcc ggcggcgcgc
     1381 tgggcttggt cttcgcgtag tggtagaccg cgtcgcccgc tgcgatcgcg agcgcctcgg
     1441 catgcgccgc ggtcgccccg ccgccggcga cggcgacgtg cttgctcggg cccgacttca
     1501 gtgccccgag cgccgccgcg gccgccgcct tgaaggcctt ggcggagccg tcgcgcgcga
     1561 ccgccagcac gaggcggccc gccttcacgc ccgccgcgcg cggcaggtac agggtcttgc
     1621 cggccttgcg ttcgaggtcg ccgcccttga cggcatcggc aatcgcttcg tcgaccacgc
     1681 tcccgaacga cggcaggtcg ccggcggcgg gcagcaccac gaccagggcg tccgcattga
     1741 cgcctgccac gcccgcgtcg ggctgggtca gggtttgaaa gtccatagta ggctcttggc
     1801 tcgattggag ataatcggtc gatgttattc gattcgaccg tccgcaagga gctggcgcgc
     1861 agcttcggcg cgacgctgat ggtcatcctc accatcttca tgaccaacct ggtcatccgc
     1921 acgctcggcc tcgcggccaa cggcgcggtg gccccgcagg acgtcgtcct catcttcacg
     1981 ctgctcgggc tccagacgct gccgacgatc ctcgcgctgt cgctgttcgt cggcgtgatc
     2041 atcagcctcg gacgcatgta tcgcgagagc gagatggcga tctggttctc cagcggcatc
     2101 ggcctgaagc gcttcgtgcg tcccgtgctg gccaccagct ggcccgtgat cgtcgtcatc
     2161 gccggcctga tgctgttcgt ctggccctgg agctatggcc agtacaacga cctgcgccag
     2221 cgcttcgagc agcgcgcgga cgtgctgcgc gcctcgcccg ggcagttcca gacctcgggc
     2281 gacggcaagc gggtgttctt cgtcgagaag gcgggctcgg cgcaggccgg cggcggccgc
     2341 aacgtgttca tcgtcaacga cctgggcggc aaggagtccg tcgcggtctc gcagtccggc
     2401 aagctccaga gcgagaacgg cgagcgcgtg ctggtcctgg acagcggcca catgcagcag
     2461 accgacaagg cgaccggcga gcacacccat gtctcgttcg accactaccg ggtgcgcgtc
     2521 ggggagacgt ccggtgcgtc caacggcacg ccgtcgccgc gccagatcga cacgagcgag
     2581 ctgctgctga ccggcggcaa tgccaacatg gccgagctca cctggcgcat cgggctgatc
     2641 ctgggcgcga ccaacctgct gttcatcggc atcagcctgt cggcgaccaa cccgcgccgg
     2701 gcgagcaact ggagcctgtt gttcgcgctg ctggccttcg tcatctacta caacctgctg
     2761 aacctgtcgc agtcttgggt cgcggccggg cgactgcccg cggcggtggc gctggtcgga
     2821 ctgcacgggc tcgcgttcgg cgtcggcatg ggcctgatct ggtggcgcga gtattcgacc
     2881 gtcgtccacc cgctgcgcct gatcctcggc cggggagcgc cgcgatgaag accgtgcgcc
     2941 agctgctgta ccgggacgtg ttctggtcgg tgttcttcgt cacggtggcc ttcgtggcgc
     3001 tgttcttctt catcgacttc gtcgacgaga tgagccgcgt cggccacggc ggctacaccg
     3061 ccaaggcggc cgcgctcgcg acgctgctcg agcagcccgg ccacgtctac gagctgttcc
     3121 cgatcgcggt gctgatcggc acgatctatt cgatggcgcg gctcgcgcag tcgtccgagt
     3181 tcacgatcct gcgcaccgcc ggcctcggcc ccgggcgcgc gctgagcctg ctgggcgtga
     3241 tcgggctgct gttcgcggtg ctgaccttcg gcctgggcga gtacgtcacg ccccacagcg
     3301 aacaggcggc ggccttcctc aaggcgcgct tcggcatggg gctggcggtc gggcgcaccg
     3361 gcgcgtggct caaggagcat cgcaccgatc ccgacggcga gcgcagcgtc accgtcaacg
     3421 tgcgcggcgt cggcaagaac ggcgagctca ccggcgtgcg catctgggag atgaacgacg
     3481 cggggcgcga ggtgcgcggc atcaccgccg agcacgcggt cgtcgggcgc gggcgcaccg
     3541 tgcgcgagtg gacgctggag aacgcgaccc agacgctctg ggacacgggc gccgcgacct
     3601 cggccgccgc gccggcctcc gcggcctcgg gcgccaccgc gccgacggtg cgcgtcacgc
     3661 atctcgacca gatgagctgg ccgacgcgcc tgaatgcctc cgtcgccgcg gccgcggtgc
     3721 tgccgatcaa cagcatgggc gcgctcgacc tgtggcgcta cagcacccac ctcgcggcgc
     3781 aggagcaggc ctcgcagacc taccgcatgc agttctggaa gcgcgtgctg tacccgttcg
     3841 cctgcctcgt gatggtggcg ctcgcgctgc ccttcgccta cctgcacgcg cgcgccggcg
     3901 gcgtcagcgt caaggtcttc ggcggcatca tgctgggcat cagcttcgtg ctgttgaaca
     3961 acgtcgtcgg ccacctcggc cagctgcacg actggacgcc ctggatcgcc gcctcggtgc
     4021 ccagcgtcgt ctacctgctg ctgtcgatgt cggccttcgc ctggctcgtg aggtaccgct
     4081 gatgacccgt gccatcgtcc tgctcgccca tggcgcccgc gacccgtcgt gggccacccc
     4141 gttcgagacc gtcgccgcgc gcgtgcgcga gaaggcaccg ctcacccagg tgcggctcgc
     4201 cttcctcgag ctgatgtcgc ccgacctcgc gaccgcgggc gacgagctcg ccgccgccgg
     4261 atgcgcgcac gtcgacgtcg tgccggtctt cctgggcggc ggcggccacg tccggcgcga
     4321 cgtccccgtg cagctcgacg cgcttcgcgc gcgccatccc caggtcgcct ggaggctgca
     4381 tcccgcgctc ggggagacgc cgcacgtgat cgccgcgctg gcggacgccg cggtcgacct
     4441 cgccgggctc gcgttcgcgg tgccgcacga cccgcaggac ggcgtcggca catgaacctg
     4501 caccagttcc gcttcgtgca ggaggccgtg cgccgcaacc tcaacctcac cgagaccgcg
     4561 aaggccctgt tcacctcgca gccggggatc tccaaggcca tcctcgagct ggaggaggag
     4621 ctcggcgtcg acctgttcgt gcgccacggc aagcgcctgc gccgcgtcac cgagccgggg
     4681 cgcgaggtgc tcaaggcgat cgacgtgatc ctgcgcgagg tcgccaacat gaagcgcatc
     4741 ggcgaggagt attccaagca ggacgccggc acgctgtcga tcgcggccac gcacacgcag
     4801 gcgcgctacg tcctgcccga gccggtggcg cagctgcgtc ggcgcttccc caaggtgcag
     4861 gtcagcctgc accagggctc gccggcggag gtcgcgcaga tgctgctgga cggcaccgcc
     4921 gacatcggcc tggccaccga gtcgctcgca gagcccgagg acctcgtcac cctgccctgc
     4981 tacgagtggg agcacgtgat cgtgatgcgc gccgacgacc cgctcgcgca ggcggacaag
     5041 atctcgctcg agcagctcgc cgagcagacg ctcatctcct accacccgag cttcaccggc
     5101 cgccgccgca tcgacgccgc cttcgcgcag cgccgcctga agcccaacat cgcgctcgag
     5161 gcgatcgact cggacgtcat caagacctac gtgcgagccg gcctgggcgt aggcatcgtc
     5221 gccgagatgg ccgtgcgcga cgacccgccc ggcggcgacc tcgtgcacaa gcccgcgggc
     5281 gtcctctttg gccgcaacgt cacgcgcgtc gcgttccgcc gcggcgccta cctgcgcggc
     5341 ttcgtgctga ccttcgcgga gctgctgtcc gaccgcatca cccgtccact gctgctgaag
     5401 gcgctgggcg gcgagggaga gaactatgaa ctctgaccgc cgcgcctcga ccgccgaatc
     5461 cctgctcgcg cgcggcgccg aggccggtca ggccgtgccg agccgcctgc cggccgtcgg
     5521 cacgaccatc ttcaccgtga tgtccgcgct tgccgcaagg accggcgcga tcaacctcgg
     5581 ccagggcttt cccgacttcg actgcgatcc cgcgctcgtc gacgcggtcg cggcggccat
     5641 gcgcgcaggc cacaaccagt acccgccgat gatcggcgtg ccggcgctgc gcgaggcgat
     5701 cgcggccaag gtggaggcga cgaccggccg ccgctacgac cccgaggcgg agatcaccgt
     5761 caccgccggc gcgacgcagg gcctgctgtc gaccatcctc gcgctggtgc acccgggcga
     5821 cgaggtcgtg gtgctggagc cgtgctacga cagctacggg ccgtccatcg agctcgcggg
     5881 cgggcgcatc gtgcgcgtcg cgctgacgcc gggcacctgg cggcccgact tcgacgccct
     5941 cgccgcggcg ctcacgccgc gcacgcgcct cgtgatcgtc aacacgccgc acaacccgag
     6001 cggcacggtc tggacgcagg acgagcgcgc gcggctcgcc gaggtgctgc gcccgacgcg
     6061 cgcgctggtg ctgtccgacg aggtctacga gcacatggtc ttcgacggcc gggcgcatgc
     6121 gagcgtctcg ggcgatccgg agctcgccga gcgcagcgtg gtcgtctcga gtttcggcaa
     6181 gaccttccac gtgacgggct ggaaggtcgg ctacgtggcc gcgccggcgg cgctgatgcg
     6241 cgagttccgc aaggtgcacc agttcaacgt gttcaccgtg aactcggcga tgcagcacgg
     6301 gctggccgcc tacctcgccg acccggggcc gtggcagggg ctcgcggcgt tctaccaggc
     6361 caagcgggat ttcttccgcg ccggcctcgc gccgacgcgc ttcaggctgc tcgcctgcga
     6421 gggcagctac ttccagtgcg tggactactc ggcgatctcc gacgagcccg acgccgcctt
     6481 ctgcgagcgc ctgaccaccg gcatcggcgt cgccgcgatc ccgatgtcgg cgttctacga
     6541 ggccgcgccc gacatgcacg tgatccgctt ctgcttcgcc aagcgcgagg aaacgctcgc
     6601 cgccgcgctg gagcgcctgc agcgtctctg acgaatccga agcgccgctg cccggccccc
     6661 cgcggcgggg acgcccggct tgtatcttcc aaacagacca gcgcggtaga gtctgaatgc
     6721 cgttgaggca accagaccgg cccgtgtagt ttgttcccgg ctagggtgtt cgagcccgtc
     6781 actgagtaaa gagcgcacgg ctgccgtgct ggtctt
//
LOCUS       DASVLN010000819         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_96763, whole genome shotgun sequence.
ACCESSION   DASVLN010000819 DASVLN010000000
VERSION     DASVLN010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96763"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VF453_19425"
     CDS             <1..599
                     /locus_tag="VF453_19425"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7689887.1"
                     /translation="GVTGLGSSTDYLTRYLVLSAGAHPGELATTGMGSGTGFIAALAA
                     GRIDAGMTSDPTVTSAVRGGHARLLVDLRTPATSEAVLGGPYPGACLYMSTSWVESHR
                     PLVRRVAAALVRALRFIASHSAEDIARAAPPPREVDLETWVAALRESKPGFTADGAMP
                     PQGPETVMRVLAVADRAVRGKPVDLARTWTGEFVADIP"
     gene            654..1334
                     /locus_tag="VF453_19430"
     CDS             654..1334
                     /locus_tag="VF453_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689888.1"
                     /translation="MRILLVEDNRTLAEWLQRTLVKDQYAVDLVDNGVDADLVLRTEA
                     YDLVILDLSLPRMDGPEVLRRLRARGNRTPVMILTANNTISSRVSELDHGADDYVAKP
                     FEVEELEARIRVLLRRSSNVANPLLECGSLRYDTNSREFSLAGEPLALPRREHAILES
                     LIMRSGTTLSKQALCQRMETLDVDVSPEAVEVYVHRVRRKLDNADVAIVTLRGLGYLL
                     RPVDRSAQ"
     gene            1384..>2747
                     /locus_tag="VF453_19435"
     CDS             1384..>2747
                     /locus_tag="VF453_19435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7689889.1"
                     /translation="MSSLRAQLLAWVLVPVGCAVAINGWIAHDSAYERADARQDTLLA
                     GAARIIAERVAVDEGQGDVPPAALELFQGDPQDAVWYRATDDRGRTLIGTAELPLPPS
                     KLQPEHPVFFRASVRGRPVRIAAYLQPIMGEPADRRAVVEVAQTLVAHDGIARRFWTH
                     GVLQQLGLLLLVTALILLGLRRGLRPVLELRDAVTRRPAGATEPLRHGNVPEEFMPLV
                     DAINAYGRRLTHYTEAQRVFIQNAAHQLRTPFTLLQTQASFALRARDDQARHESLIAI
                     RRTIHQSARLVNQLLVLSAAEARADGERRLDVVDLRPLVQEVLEDLAAAAEAKRIDLG
                     LDEADADVRALGRRVALREIFMNLVDNAIRYTPAGGIVTVRLTRDTDRATVVVEDDGP
                     GIPQAQREFVFERFARLSDSDSDGCGLGLAVVRELATALDARVELATATGGRGLAAIV
                     RLAAA"
BASE COUNT          365 a         1073 c          962 g          347 t
ORIGIN      
        1 tcggcgtcac cggcctgggc tcgtcgacag actacctcac ccggtacctg gtgctcagcg
       61 ccggcgcgca cccgggcgag ctcgcgacga ccggcatggg ctcgggcacc ggcttcatcg
      121 cggcgctcgc cgcgggccgc atcgacgccg gcatgaccag cgatccgacc gtcacgtcgg
      181 cggtgcgcgg cggccatgcg cgcctgctcg tcgacctgcg cacgcccgcg accagcgagg
      241 ccgtgctcgg cggcccctat cccggcgcat gcctgtacat gtcgaccagc tgggtcgagt
      301 cgcaccggcc gctggtgcgc cgcgtcgcag cggcgctggt gcgcgcgctg cgcttcatcg
      361 cctcgcacag cgcggaagac atcgcccgcg ccgccccgcc gccgcgcgag gtcgacctcg
      421 agacctgggt cgccgcgctg cgcgagagca agcccggctt caccgccgac ggcgcgatgc
      481 cgccgcaggg gccggagacc gtcatgcgcg tgctggccgt ggccgaccgc gcggtgcgcg
      541 gcaagccggt cgacctcgcc cgcacctgga cgggcgagtt cgtcgccgac atcccctgag
      601 gcccgcggac gactccgaca cgcccggccc atccacaggc tccgattccg cccatgcgca
      661 tcctcctcgt cgaagacaac cgcaccctgg ccgagtggct gcagcgcacg ctggtgaagg
      721 accagtacgc cgtcgacctc gtcgacaacg gcgtcgacgc cgacctcgtg ctgcgcaccg
      781 aggcctacga cctcgtgatc ctcgacctct cgctgccgcg catggacggc cccgaggtgc
      841 tgcgccggct gcgcgcgcgc ggcaaccgca cgcccgtgat gatcctgact gccaacaaca
      901 cgatcagcag ccgcgtgagc gagctcgacc acggcgcgga cgactacgtg gccaagccct
      961 tcgaggtcga ggagctggag gcgcgcatcc gcgtgttgct gcggcgctcg tcgaacgtcg
     1021 ccaacccgct gctggaatgc ggcagcctgc gctacgacac caattcgcgc gagttctcgc
     1081 tcgccggcga gccgctcgcc ctgccgcgac gcgagcacgc gatcctcgag tcgctgatca
     1141 tgcgctcggg cacgacgctg tccaagcagg cgctctgcca gcgcatggag acgctggacg
     1201 tcgacgtctc gcccgaggcg gtcgaggtct acgtccaccg cgtgcgccgc aagctggaca
     1261 acgccgacgt cgcgatcgtc acgctgcgcg gcctcggcta cctgctgcgg cccgtcgacc
     1321 ggagcgcgca gtgagcgcac gacgggcgct cgcaccgcag gccgcccggc cggaggcatc
     1381 gcgatgagca gcctgcgcgc gcagctgctc gcgtgggtgc tcgtgcccgt gggatgcgcc
     1441 gtcgcgatca acggctggat cgcgcacgac agcgcctacg agcgagccga cgcgcgccag
     1501 gacacgctgc tggccggcgc cgcgcgcatc atcgcggagc gcgtcgcggt cgacgagggc
     1561 cagggcgacg tgccgccggc ggcgctggag ctgttccagg gcgatccgca ggacgccgtc
     1621 tggtaccgcg ccaccgacga ccgcggccgc acgctgatcg gcacggcgga gctgccgctg
     1681 ccgccgtcga agctccagcc cgagcacccg gtcttcttcc gcgcgtcggt gcgcgggcgc
     1741 ccggtgcgga tcgccgccta cctgcagccg atcatgggcg agccggccga ccggcgcgcg
     1801 gtcgtggagg tcgcgcagac cctcgtcgcc cacgacggca tcgcccgccg cttctggacc
     1861 cacggcgtgc tgcagcagct cggcctcctg ctgctcgtga cggcgctcat cctgctgggc
     1921 ctgcggcgcg gcctgcgccc ggtgctggag ctgcgcgacg ccgtcacgcg ccgccccgcc
     1981 ggcgccaccg agccgctgcg ccacggcaac gtgcccgagg agttcatgcc gctggtcgac
     2041 gcgatcaacg cctacggccg tcgcctgacg cactacaccg aagcccagcg cgtcttcatc
     2101 cagaacgccg cgcaccagct gcgcacgccg ttcacgctgc tgcagacgca agccagcttc
     2161 gcgctgcgcg cgcgcgacga ccaggcgcgc cacgagtcgc tgatcgccat ccgccgcacc
     2221 atccaccaat cggcgcggct cgtgaaccag ctgctcgtgc tgtcggcggc cgaggcgcgc
     2281 gccgacggcg agcggcggct ggacgtcgtc gacctgcggc cgctggtgca ggaagtgctc
     2341 gaggacctcg ccgccgccgc ggaggccaag cgcatcgacc tcggcctgga cgaggcggac
     2401 gccgacgtgc gcgccctggg ccggcgcgtg gcgctgcgcg agatcttcat gaacctcgtc
     2461 gacaacgcga tccgctacac gccggccggc ggcatcgtca ccgtgcgcct cacgcgcgac
     2521 acagaccgcg cgaccgtggt cgtcgaggac gacggtcccg gcatcccgca ggcgcagcgc
     2581 gagttcgtct tcgagcgctt cgcccgcctg agcgacagcg actccgatgg ctgcggcctg
     2641 ggcctggccg tggtgcgcga gctcgcgacc gcgctggacg cgcgcgtgga gctcgcaacc
     2701 gcgaccggcg gccgcggcct cgcggcgatc gtgcggctgg cggcggc
//
LOCUS       DASVLN010000820         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_258504, whole genome shotgun sequence.
ACCESSION   DASVLN010000820 DASVLN010000000
VERSION     DASVLN010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258504"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VF453_19440"
     CDS             <1..874
                     /locus_tag="VF453_19440"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7689890.1"
                     /translation="AAPRAHAHRIAILLSDLRNPFFATIARSFEQRLRALDPGDGPLD
                     VLSAGFDPERQAQQLAAQLRAAVDLVVVTPIDARSLGPHVAEVRRAGMRVIAVDSRVE
                     GADLVITTDNAAAAEMACDELVAQLHGQGRIAIVDGPPNSADIDRLQGCDRVLRRHPG
                     ISVVARTSNAGGSREGGLERMVAVMAANPRIDGVFAINDLTALGVEQAAREAGRDEMI
                     VTSVDGSPAVVERLKDARSLIAASASQDPARMGVLAAQGAAALLRGQAPAQPQVLLKP
                     TLVTRANVTRYRGW"
     gene            892..>3113
                     /locus_tag="VF453_19445"
     CDS             892..>3113
                     /locus_tag="VF453_19445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX7689891.1"
                     /translation="MAAASDNAGRFPLRIRRGSLRRRWFVAITILGGATALLAAAGVV
                     ALRDTGRQLASYNEQVLPDVAKSLELAERVSHLAATAPYVAEAAIASKLNQESAELKK
                     LQAEVGRQAVSPSLRAQTSDLSPVLSALQTSIDELIRVKREDLFLREDLRAQLFVLDE
                     LRTRARAARPACPASIELASRTAQLAAGALGTTEVDQLRDEFAASLGAMRGPDAARCE
                     ADRLSAAATAIFGLRRSVLAQEDRKAYLLAYTRVLSDSVTASANRHVARIERELSERQ
                     AAIQLAIRTGTMGIAIVSAVALVVLLGTAFVVRRSLAQLDSVTRAMTSLAARDAHGGA
                     GEAASHDEVAQLIAAFDVFRDNALAMRGMAASLSEQTRLLETVFDSINDGLSVFDRDH
                     RLVAWNPRYLELLELPASAVRAGSRLEDIQALVRLEVKPAADAPGSAIGEWNARRARS
                     PHSLEATLGSGRILSLRSQPMPDGGFVTLYGDLTERRAVDAQLRQSQKMEVLGQLTGG
                     VAHDFNNLLAAIMSNLQLLEAEASLSPAVQRIVGRAAKASERAASLTRRMLAFARRQP
                     LRPEAVDVDAAIAELRDLVKHSAGHAIRIEMSHGAGGALVSIDRGQLENALLNLTLNA
                     GAAMPHGGELRFVTRVVDAGERVHPAGMAVEIEVADTGVGMSDAVRERIFEPFFTTKR
                     SEGSGLGLSIVYGFVKQSGGDVRVASRPGEGTRFTIVLPVLAQAPVRLPAPASAASQV
                     P"
BASE COUNT          392 a         1186 c         1141 g          394 t
ORIGIN      
        1 ggccgcgccc cgggcgcatg cgcaccgcat cgcgatcctg ctgtccgact tgcgcaaccc
       61 gttcttcgcc acgatcgcgc gcagcttcga gcagcgcctg cgcgcgctcg acccgggcga
      121 cgggccgctg gacgtcctgt ccgccggctt cgacccggag cgccaggcgc agcagctcgc
      181 ggcccagctg cgcgccgcgg tcgacctcgt cgtcgtgacg cccatcgacg cccgctcgct
      241 cggcccgcac gtggccgagg tgcgccgcgc cggcatgcgc gtgatcgccg tcgactcgcg
      301 cgtggagggc gccgacctcg tcatcaccac cgacaacgcc gccgccgccg agatggcctg
      361 cgacgagctc gtcgcgcaac tgcacgggca gggccggatc gcgatcgtcg acggcccgcc
      421 caacagtgcc gacatcgatc gcctgcaggg gtgcgaccgc gtgctgcggc gccacccggg
      481 catcagcgtc gtggcgcgca cctcgaacgc gggcggctcg cgcgagggcg gcttggagcg
      541 catggtcgcc gtgatggcgg ccaacccgcg catcgacggc gtgttcgcga tcaacgacct
      601 cacggcgctg ggcgtcgagc aggcggcgcg cgaggccggc cgcgacgaga tgatcgtcac
      661 cagcgtcgac ggctcgcccg cggtcgtcga gcgcctgaag gacgcgcgtt cgctcatcgc
      721 cgccagcgcc tcgcaggatc cggcgcgcat gggcgtgctc gcggcgcagg gcgcggcggc
      781 gctgctgcgc ggccaggcgc ccgcgcagcc gcaggtgttg ctgaagccga cgctcgtgac
      841 gcgcgccaac gtcacgcgct atcgcggctg gtgacgcggg acgcgacgac catggccgcg
      901 gcctccgaca acgcgggacg cttcccgctg cgcatccgcc gcggcagcct gcggcggcgc
      961 tggttcgtgg cgatcaccat cctcgggggc gcgacggcgc tgctcgccgc ggcgggcgtc
     1021 gtcgcgctgc gcgacaccgg acgacagctc gcgtcctaca acgagcaggt gctgcccgac
     1081 gttgccaagt cgctggagct cgcggagcgg gtctcgcacc tcgccgccac cgcgccctac
     1141 gtcgcggagg ccgcgatcgc ctccaagctg aaccaggaga gcgccgagct gaagaagctg
     1201 caggccgagg tcgggcggca ggccgtctcg ccgtcgctgc gggcgcagac gagcgacctg
     1261 tcgccggtgc tgtcggcgct gcagaccagc atcgacgagc tgatccgcgt caagcgcgag
     1321 gacctgttcc tgcgcgagga cctgcgcgcg cagctgttcg tgctcgacga gctgcgcacg
     1381 cgcgcgcgcg cggcgcggcc ggcgtgcccg gcctcgatcg agctcgcgtc gcgcaccgcg
     1441 cagctcgcgg ccggggcgct cggcacgacg gaggtcgacc agctgcgcga cgagttcgcg
     1501 gccagcctcg gcgcgatgcg cgggccggat gccgcgcgct gcgaggcgga ccggctctcg
     1561 gccgcggcga cggcgatctt cgggctgcgg cgctcggtgc tcgcgcagga ggatcgcaag
     1621 gcctacctgc tcgcctacac gcgcgtgctg tccgattcgg tgacggcctc cgccaaccgg
     1681 cacgtcgccc gcatcgagcg cgagctctcg gagcgccagg ccgcgatcca gctggccatc
     1741 cgcaccggca ccatgggcat cgcgatcgtc tccgcggtcg cgctggtggt gctgctcggc
     1801 accgccttcg tcgtgcggcg ctcgctcgcg cagctcgact cggtgacgcg cgcgatgacg
     1861 agcctcgcgg cgcgcgacgc ccacggcggc gccggcgagg cggcgagcca cgacgaggtc
     1921 gcgcagctga tcgccgcgtt cgacgtcttc cgcgacaacg cgctcgcgat gcgcggcatg
     1981 gcggcgagcc tgtcggagca gacgcggctg ctggagacgg tcttcgacag catcaacgac
     2041 ggcctgagcg tgttcgaccg cgaccaccgg ctggtggcgt ggaacccgcg ctacctcgag
     2101 ctgctggagc tgcccgcctc cgccgtgcgc gccggcagcc ggctcgagga catccaggcc
     2161 ctggtgcggc tggaggtcaa gcccgccgcc gacgcgcccg gctcggcgat cggcgaatgg
     2221 aacgcgcggc gcgcgcgttc gccgcactcg ctggaggcga cgctgggcag cggccgcatc
     2281 ctcagcctgc gcagccagcc gatgcccgac ggcggcttcg tcacgctcta cggcgacctc
     2341 accgagcgcc gcgccgtcga cgcgcagctg cgccagagcc agaagatgga ggtgctgggc
     2401 cagctcaccg gcggcgtcgc gcacgacttc aacaacctcc tcgcggccat catgagcaac
     2461 ctgcagctgc tggaggccga ggcctcgctg tcgccggccg tccagcgcat cgtcggccgc
     2521 gcggccaagg cctccgagcg cgccgcctcg ctgacgcgcc gcatgctcgc cttcgcccgc
     2581 cggcagccgc tgcggccgga ggcggtcgac gtcgacgccg ccatcgccga gctgcgcgac
     2641 ctcgtcaagc actcggccgg gcacgcgatc cgcatcgaga tgtcgcacgg cgccggcggc
     2701 gcgctcgtct ccatcgaccg cggccagctc gagaacgccc tgctgaacct gacgctcaac
     2761 gccggcgcgg cgatgcccca cggcggcgag ctgcgcttcg tcacgcgcgt cgtcgacgcc
     2821 ggcgagcggg tgcatccggc cggcatggcg gtggagatcg aggttgcgga tacgggggtg
     2881 ggcatgtccg acgccgtgcg cgagcgcatc ttcgagccct tcttcacgac caagcgctcc
     2941 gagggcagcg gcctcgggct gagcatcgtc tacggcttcg tcaagcagag cggcggcgac
     3001 gtgcgcgtgg cgagccggcc gggcgagggc acgcgcttca cgatcgtgct gcccgtgctc
     3061 gcgcaggcgc ccgtgcgcct gcccgcgccc gcgtcggccg cctcgcaggt gcc
//
LOCUS       DASVLN010000821         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_96928, whole genome shotgun sequence.
ACCESSION   DASVLN010000821 DASVLN010000000
VERSION     DASVLN010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96928"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VF453_19450"
     CDS             complement(<1..336)
                     /locus_tag="VF453_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX7689892.1"
                     /translation="MGNKLYVGNLAYSVRDESLQEAFGQFGTVTSAKVMMDRETGRSK
                     GFGFVEMGSDAEAQAAINGMNGQPLEGRPVVVNEARPREERPGGFSGAPRSPYGGGGR
                     SGGGGYGGGG"
     gene            691..1332
                     /locus_tag="VF453_19455"
     CDS             691..1332
                     /locus_tag="VF453_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX7689893.1"
                     /translation="MDLIKPLIALLAIVNPVGAIPFFLHFTQNLTRQQRQRTIRVSSL
                     SACLVVAVSAVAGLKIIDFFGISIASFQVGGGLLLLISSMNMLNAQQAESRGTDVGDG
                     QAKADAGDSIAVVPLTIPLLTGPATISTMVIYAEKTRTLVEHAVLVGYGVVIGLATFL
                     VFTASGRIARVLGRTGINVMTRLMGLILAAMAVELLADGLTKLFPALGRAMTN"
     gene            1329..>1639
                     /locus_tag="VF453_19460"
     CDS             1329..>1639
                     /locus_tag="VF453_19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7689894.1"
                     /translation="MNARILLLEDDPAIAGTVAFALQREGFEVEHAPLAGLARRSALA
                     GAPALAILDVGLPDGNGLDLCREWRQGDSPALRTLPVLMLTARAEELDRVVGLELGAD
                     D"
BASE COUNT          260 a          568 c          536 g          275 t
ORIGIN      
        1 accgccgcca ccgtagccgc cgccacccga gcggccaccg ccgccgtagg ggctgcgcgg
       61 ggcgccgctg aagccgcccg gacgctcttc acgaggacgc gcctcgttca cgaccacggg
      121 acggccttcc aggggctggc cgttcatgcc gttgatggcg gcttgcgcct cggcatccga
      181 acccatctcc acgaagccga agcccttgga gcggccggtt tcgcgatcca tcatgacctt
      241 ggcagacgtg acggtgccga actggccgaa cgcttcttgc agcgactcat cgcgcacgct
      301 gtaggcgagg ttgcctacgt acagtttgtt tcccactggg gagccctttc agagaaatag
      361 aaccatgtgg agcttcaacc cagcgtgaac catcaagcga aggcgccgcg tccagacaca
      421 gcatcgacgg gagacgattg ctcgaagcga ccgccgccct cgaattgatt atgggcccaa
      481 cgaaccacgg aaagcaaagc gcagtcaagg aatttgttgt tttgtcgttg ccgagcggtg
      541 aatgctgtgt tttccccatc agatggcgtg tgaaggagcc gtggaaccag ggtttcaact
      601 gacgggtttt cccggaaaag cgcgtcatcg cgttgcccgg gagcgcgaag taccgtgcat
      661 gaatcgtccg cagcgaggcg agaatcggcc atggacctga tcaagcccct gattgccctg
      721 ctcgcgatcg tcaaccccgt gggcgcgatc ccgttcttcc tgcacttcac gcagaacctc
      781 acccgccagc agcgccagcg cacgatccgc gtctcctcgc tcagcgcctg cctggtcgtg
      841 gcggtcagcg ccgtggcggg cctgaagatc atcgacttct tcggcatctc gatcgcctcc
      901 ttccaggtcg gcggcgggtt gctgctgctg atctcgtcga tgaacatgct caacgcgcag
      961 caggccgaga gccgcggcac cgacgtcggc gacggccagg cgaaggccga cgccggcgac
     1021 tcgatcgccg tggtgccgct gacgatcccg ctgctgaccg gcccggcgac gatctcgacc
     1081 atggtcatct atgccgagaa gacgcgcacg ctggtcgagc acgccgtgct ggtggggtac
     1141 ggcgtcgtca tcgggctcgc gaccttcctc gtgttcacgg cctcgggccg catcgcgcgc
     1201 gtgctcggcc gcaccggcat caacgtgatg acccggctga tgggcctgat cctcgccgcg
     1261 atggcggtcg agctcctcgc cgacggcctg accaagctct ttcccgcgct cggccgcgcg
     1321 atgacgaatt gaacgcgcgc atcctgctgc tggaggacga cccggccatc gccggcacgg
     1381 tggcgttcgc gctgcagcgg gagggcttcg aggtcgagca tgcgccgctg gccggcctcg
     1441 cccggcgcag cgcgctcgcg ggcgcgcccg cgctggccat cctcgacgtc ggcctgcccg
     1501 acggcaacgg cctcgatctc tgccgcgagt ggcggcaggg cgactcgccc gcgctgcgca
     1561 cgctgccggt gctgatgctg acggcacgcg ccgaggagct cgaccgcgtc gtcgggctgg
     1621 agctcggcgc cgacgatta
//
LOCUS       DASVLN010000822         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_41513, whole genome shotgun sequence.
ACCESSION   DASVLN010000822 DASVLN010000000
VERSION     DASVLN010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41513"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="VF453_19465"
     CDS             <1..808
                     /locus_tag="VF453_19465"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7689895.1"
                     /translation="DRHAERRGDGLAFVHDVAHGGLDAGPAAIAGAALVLVCVKSTDT
                     ASAADAMRAHLAPGARVLSLQNGVDNAPRLEAALGVPVAPAVVYVASEMAGPGHVRHM
                     GRGELVIPDALDGAAGLTSGEIVALFAGAGVPVETSGNVVGALWAKLVLNCAYNALSA
                     ITDTPYGRLVALAGIEATMRDVVDECQAVAAALGVAIPGDVRESVARIARTMPGQVSS
                     TCQDLRRGKPSEIDHLNGHVAAEGARLGVATPVNRALHAIVKALEARRAG"
     gene            complement(777..1037)
                     /locus_tag="VF453_19470"
     CDS             complement(777..1037)
                     /locus_tag="VF453_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689896.1"
                     /translation="MEPRRLRTIVAWVCTVGGMLAAASYALFPLTVGGTLGQGVGMLL
                     VGLVLVLPALLLAAVVAVFHLRGEPDDWDPPTLIPPAAPPAP"
     gene            complement(1042..1518)
                     /locus_tag="VF453_19475"
     CDS             complement(1042..1518)
                     /locus_tag="VF453_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HEX7689897.1"
                     /translation="MNAYLALKTVHVISSVLLAGTGFGTAFYLFFANRTPSLEARVVV
                     SRLVVRADTWFTTPAAIVQPLSGFALAHMAGWPLTTPWLVASMGLYAVAGLCWLPVVW
                     LQVRMARTAAADLAAGRPLSPSYARDARLWEALGYPAFVAMLAIYVLMVNKPALWG"
     gene            complement(1515..>1745)
                     /locus_tag="VF453_19480"
     CDS             complement(1515..>1745)
                     /locus_tag="VF453_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HEX7689898.1"
                     /translation="PAALALWGGVAADLGIGLWLWLRPSRAAVDAALAATAVLTIVAT
                     VLLPGLWLHPLGPLLKNLPIVAALVVLRRSLW"
BASE COUNT          261 a          618 c          674 g          192 t
ORIGIN      
        1 ggatcgccac gccgagcgcc gcggcgacgg cctggcattc gtccacgacg tcgcgcatgg
       61 tggcctcgat gccggccctg cggcgatcgc cggcgcggcg ctcgtgctgg tgtgcgtgaa
      121 gtcgaccgac accgcgtcgg cagcggacgc catgcgcgcg cacctggcgc cgggcgcacg
      181 ggtgctcagc ctgcagaacg gcgtcgacaa cgcgccgcgg ctcgaggcgg cgctgggcgt
      241 gccggtcgcg ccggcggtgg tctatgtcgc cagcgagatg gccgggcccg gccacgtgcg
      301 gcacatgggc cgcggcgaac tcgtgatccc ggacgcgctc gatggcgcgg cggggctgac
      361 gtccggcgag atcgtcgccc tgttcgcggg cgccggcgtg ccggtcgaga cctcgggcaa
      421 cgtcgtcggc gcgctctggg ccaagctggt gctgaactgc gcctacaacg cgctctcggc
      481 gatcaccgac acgccctacg gtcgcctcgt cgcgctggcc ggcatcgagg ccaccatgcg
      541 cgacgtcgtg gacgaatgcc aggccgtcgc cgcggcgctc ggcgtggcga tcccgggcga
      601 cgtgcgcgaa tcggttgccc gcatcgcgcg cacgatgcct gggcaggtct cgtccacctg
      661 ccaggacctg cgccgcggca agcccagcga gatcgaccac ctgaacggcc atgtcgccgc
      721 cgagggcgcg cgcctgggcg tcgcgacgcc ggtcaaccgg gcgctgcacg cgatcgtcaa
      781 ggcgctggag gcgcggcggg cgggatgagc gtgggcgggt cccagtcgtc cggctcgccg
      841 cgaaggtgga agacggcgac caccgcggcg agcagcagcg cgggcagcac caggaccagg
      901 ccgacgagca gcatgcccac gccttgtccc agcgtgccgc cgacggtcag cgggaagagc
      961 gcgtacgacg cggcggccag catcccgccg acggtgcaga cccaggcgac gatcgtgcgc
     1021 aagcggcgcg gttccatggc ctcatcccca cagcgccggc ttgttgacca tcagcacgta
     1081 gatggcgagc atcgcgacga aggccgggta gccgagggcc tcccagaggc gggcgtcgcg
     1141 cgcgtaagac gggctcaatg ggcggccggc ggcgaggtcg gcggccgcgg tgcgggccat
     1201 gcgcacctgc agccagacga cgggcagcca gcacaggccg gcgacggcgt agaggcccat
     1261 cgacgccacg agccacggcg tcgtcagcgg ccagccggcc atgtgcgcga gcgcgaaacc
     1321 ggacagcggc tgcacgatgg cggccggcgt cgtgaaccag gtgtcggcgc gcacgacgag
     1381 gcggctgacg acgacgcgtg cctcgaggct cggcgtgcgg ttcgcgaaga acaggtagaa
     1441 cgccgtgccg aagccggtgc cggccagcag cacgctggag atcacgtgga cggtcttgag
     1501 ggcgaggtac gcgttcacca gaggctcctt cgcagcacca ccagggccgc gacgatgggc
     1561 aggttcttca acagcgggcc gagcggatgc agccacagcc cgggcagcag gacggtcgcg
     1621 acgatcgtca gcaccgccgt ggcggcgagc gcggcgtcga ccgcggcgcg gctgggtcgc
     1681 agccacagcc acaggccgat cccgaggtcg gccgcgacgc cgccccacag cgcgagcgcc
     1741 gcggg
//
LOCUS       DASVLN010000823         5210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_41565, whole genome shotgun sequence.
ACCESSION   DASVLN010000823 DASVLN010000000
VERSION     DASVLN010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41565"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF453_19485"
     CDS             <1..366
                     /locus_tag="VF453_19485"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7689899.1"
                     /translation="ALRRFEEAAQALERAVALAPAHVEAWFSLSLALQDLRRLDDAAA
                     ALERVLSLQPGRPDALVNLGIVRQEQGRLDDAMRAYGRAYRRHEDTFGRIAHALATPG
                     TGALWLDLDALKAALRAAV"
     gene            complement(367..1071)
                     /locus_tag="VF453_19490"
     CDS             complement(367..1071)
                     /locus_tag="VF453_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689900.1"
                     /translation="MNIVFRCDPALIDRLPRPMPARAALPGWLRAMAPRVPSPVHGRE
                     IRTVKQCPPFVDAMSHGFVLPLPCDVVVERGMKFTWHWDLPEPTVPGHPRAPLSFHVP
                     AQLAGSPLAHGGRSALKFNSFWTVELEPGWSLLAMHPVNRDDLPFRLVTGLVDADRFH
                     DVGINFPAVWIDPDFEGVLARGTPVAQCCVVPREAPGLVVEAMGEAQVAGYEALANRI
                     MGGPGVYRKGYRSRQR"
     gene            complement(1196..2929)
                     /locus_tag="VF453_19495"
     CDS             complement(1196..2929)
                     /locus_tag="VF453_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7689901.1"
                     /translation="MQMRFHAVAFAAAALVAGQGAWAGEAEAKKWIDSEFQPSTLSKD
                     QQMAEMKWFIDAAAKLKARGVKEVSVVSETLTVHEYESKTLAKAFEDITGIKVKHEIM
                     QEGDVVEKLQTSMQSGKSIYDGWINDSDHIGTHYRYGTAWALTDQMAGPWKEFTDPQL
                     DLKDFIGTSFTTAPDGKMYQLPDQQFANLYWFRADLFARPDLKAKFKAKYGYDLGVPV
                     NWSAYEDIAEFFTDDVKTIDGKPIYGHMDYGKKDPSLGWRFTDAWLSMAGEADVGIPN
                     GLPVDEWGIRVADDKCTPVGASVERGGGTNSPAAVYATTKYIDWMKKYAPKQAMGMTF
                     GEAGPVPAQGQIAQQIFWYTAFTADMIKPGLPVVNADGTPKWRMAPSPHGPYWKEGMQ
                     NGYQDVGSWTFLKSADPNRELGAWLYAQFVTSKTVSLKKSITGLTFIRDSDIHSDYFT
                     KNAAKYGGLIEFYRSPARVAWTPTGNNVPDYPKLAQLWWKNVAVAVTGEKTPQQAMDS
                     LAGEMDDVMARLQRAGMAHCAPKLNPKSAPSKWLSNKEAPWAKLPNEKPKGETISYDT
                     LLNAWKAGKVR"
     gene            complement(3024..3332)
                     /locus_tag="VF453_19500"
     CDS             complement(3024..3332)
                     /locus_tag="VF453_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HEX7689902.1"
                     /translation="MFAWMEWTLPVAVFFVCIALMLVGMTIWEIRSPTTLRRGFLPIA
                     TTRGDRLFIGLMAAAWVNLGFVAMGEKLMTWLNLEQEPSVWWSFGLSMALLALIMRKG
                     "
     gene            complement(3352..4164)
                     /locus_tag="VF453_19505"
     CDS             complement(3352..4164)
                     /locus_tag="VF453_19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7689903.1"
                     /translation="MDDRRFKKRTLFLVLYLAFAILPVYWMVNMSVKTNEEILGAFSL
                     WPRHFTWDNYRTIFTDEAWYSGYINSLEYVAMNTVISLLVALPAAYAFSRYSFLGDKH
                     VFFWLLTNRMTPPAVFLLPFFQLYTTLGLMDTHIAVALAHLLFNVPLAVWILEGFMSG
                     IPREIDETAYIDGYSFPRFFLTIFVPLIKAGIGVAAFFCFMFSWVELLMARTLTSVNA
                     LPIVAVMTRTVSAAGMDWGTLAAAGVITIVPGAIVIWFVRNYIAKGFAMGRV"
     gene            complement(4157..5044)
                     /locus_tag="VF453_19510"
     CDS             complement(4157..5044)
                     /locus_tag="VF453_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7689904.1"
                     /translation="MIKPVNQKAWLMILPVVVCVAFSAVLPLMTVVNYSVQDIISPER
                     HVFVGTDWFKQVMRDDDLHGALVRQLVFSLSVLLVEIPLGIALALAMPARGWKASAVL
                     VVVAISLLIPWNVVGTIWQIFGRTDIGLLGKALTSMGFDYDYTGNERHAWFTVLTMDV
                     WHWTPLVALLCYAGLRAIPDAYYQAASIDGASKLAVFRYIQLPKMRGVLMIAVLLRFM
                     DSFMIYTEPFVLTGGGPGDATTFLSQYLTQKAVGQFDLGPAAAFSLIYFLIILLLCFV
                     LYTWMQRVGTVAEGESTHG"
BASE COUNT          840 a         1764 c         1789 g          817 t
ORIGIN      
        1 gcgctgcgcc gcttcgaaga ggccgcgcag gcgctggagc gcgccgtcgc cctcgcgccg
       61 gcgcacgtgg aggcatggtt ctcgctgtcg ctcgcgctgc aggacctgcg ccgcctggac
      121 gacgccgcgg cagcgctgga gcgcgtgctg tcgctgcagc ccggccggcc cgacgcgctg
      181 gtcaatctcg gcatcgtgcg ccaggagcag ggccggctcg acgacgcgat gcgggcctac
      241 ggccgcgcgt accgtcgcca cgaagacacc ttcgggcgca tcgcccacgc gctcgcgacg
      301 ccgggcaccg gcgcgttgtg gctggacctc gacgcgctca aggccgcgct gcgcgccgcc
      361 gtctgatcag cgctgccggc tgcgataccc cttgcggtac acgccggggc cgcccatgat
      421 gcggttggcc agcgcctcgt agcccgcgac ctgcgcctcg cccatcgcct cgaccaccag
      481 ccccggcgcc tcgcgaggca ccacgcagca ttgggcgacc ggcgtgccgc gggcaagcac
      541 gccctcgaag tccggatcga tccagacggc cgggaaattg atgccgacgt cgtggaaccg
      601 gtcggcgtcg acgagccccg tgacgagccg gaacggcagg tcgtcgcggt tgaccgggtg
      661 catcgcgagc agcgaccagc ccggctcgag ctcgacggtc cagaagctgt tgaacttcag
      721 cgccgaccgc ccgccgtgcg ccaggggcga gccggcgagc tgcgcgggga cgtggaagct
      781 cagcggcgcg cgcggatggc cgggcaccgt gggctccggc aggtcccagt gccaggtgaa
      841 cttcatcccg cgctcgacga ccacgtcgca tggcagcggc agcacgaagc cgtgcgacat
      901 cgcgtcgacg aagggcgggc actgcttgac ggtacggatc tcgcgcccgt gcaccggcga
      961 gggcacgcgg ggcgccatcg cgcgcagcca gccgggcagc gcggccctcg cgggcatggg
     1021 tcgcggcagc ctgtcgatca gcgcgggatc gcaacggaag acgatgttca tggcgaggcc
     1081 gaggatacgc gagcgcgcgt cggcagcgac ggaaaacgcc ccgccggctc gcaccggcgg
     1141 ggcgctgaag ggccgcgacc gacccttcac caggccgcgg cttggagaca agcggtcagc
     1201 gcaccttgcc ggccttccag gcgttcagca gcgtgtcgta ggagatggtc tcgcccttgg
     1261 gcttctcgtt gggcagcttg gcccacggcg cctccttgtt gctcagccac ttcgagggcg
     1321 cgctcttcgg gttgagcttg ggcgcgcaat gcgccatgcc ggcgcgctgc aggcgggcca
     1381 tcacgtcgtc catctcgccc gccaggctgt ccatcgcctg ctgcggcgtc ttctcgccgg
     1441 tgacggccac cgccacgttc ttccaccaga gctgcgcgag cttggggtag tcggggacgt
     1501 tgttgccggt cggcgtccac gccacgcgcg cggggctgcg gtagaactcg atcaggccgc
     1561 cgtacttggc cgcgttcttc gtgaagtagt cggagtggat gtccgagtcg cggatgaacg
     1621 tgaggccggt gatcgacttc ttcagcgaca ccgtcttgga cgtcacgaac tgcgcgtaca
     1681 gccacgcgcc gagctcgcgg ttcggatcgg ccgacttcag gaacgtccac gaaccgacgt
     1741 cctggtagcc gttctgcatg ccttccttcc agtaggggcc gtgcgggctc ggggccatgc
     1801 gccacttggg cgtgccgtcc gcgttcacga cgggcaggcc cggcttgatc atgtccgcgg
     1861 tgaaggcggt gtaccagaag atctgctggg cgatctggcc ctgcgcgggc accgggccgg
     1921 cctcgccgaa ggtcatgccc atcgcctgct tgggcgcgta cttcttcatc cagtcgatgt
     1981 acttggtcgt cgcgtacacc gccgcgggcg agttcgtgcc gcccccgcgc tcgaccgacg
     2041 cgcccaccgg cgtgcacttg tcgtcggcga cgcggatgcc ccactcgtcg accggcaggc
     2101 cgttggggat gcccacgtcg gcctcgccgg ccatcgacag ccaggcgtcg gtgaagcgcc
     2161 agcccagcga cggatccttc ttgccgtagt ccatgtggcc gtagatcggc ttgccgtcga
     2221 tcgtcttcac gtcgtcggtg aagaactcgg cgatgtcctc gtaggcgctc cagttcaccg
     2281 gcacgccgag gtcatagccg tacttggcct tgaacttcgc cttgaggtcc ggacgcgcga
     2341 acaggtcggc gcggaaccag tacaggttgg cgaactgctg gtcgggcagc tggtacatct
     2401 tgccgtccgg cgccgtcgtg aagctggtgc cgatgaagtc cttcaggtcc agctgcgggt
     2461 ccgtgaactc cttccacggg ccggccatct ggtcggtcag cgcccaggcc gtgccatacc
     2521 ggtagtgggt accgatatgg tccgagtcgt tgatccagcc gtcgtagatc gacttgccgg
     2581 actgcatcga ggtctgcagc ttctcgacga cgtcgccctc ctgcatgatc tcgtgcttga
     2641 ccttgatgcc cgtgatgtcc tcgaaggcct tcgcgagcgt cttggattcg tactcgtgca
     2701 cggtcagcgt ctcggagacg accgacacct ccttgacgcc gcgggccttg agcttcgcgg
     2761 cggcgtcgat gaaccacttc atctccgcca tctgctggtc cttgctcagc gtcgagggct
     2821 ggaactcgct gtcgatccat ttcttggcct ctgcctcgcc ggcccacgcg ccctggccgg
     2881 cgaccagggc ggcggccgca aaggccacgg catgaaagcg catctgcatt gctgtctcct
     2941 aggtgccttc cccgtttggt gtgacggaga cggccgggga aggacccgcc gtcgccgctg
     3001 tgatgtcgcg cctcgcgcgg cgcctagccc ttgcgcatga tcagcgccag cagcgccatg
     3061 gacaggccga agctccacca gaccgagggc tcctgctcga ggttgagcca ggtcatcagc
     3121 ttctcgccca tcgcgacgaa gccgaggttg acccacgcgg ccgccatgag gccgatgaac
     3181 aggcggtcgc cgcgcgtcgt ggcgatcggc aggaaaccgc gccgcagggt cgtgggcgag
     3241 cggatctccc agatcgtcat gcccacgagc atcagcgcga tgcagacgaa gaagacggcg
     3301 accggcaggg tccattccat ccaggcgaac attgcagtcc tttcgaagtc gtcagacgcg
     3361 ccccatcgcg aagcccttgg cgatgtagtt gcgcacgaac cagatgacga tggcgcccgg
     3421 cacgatggtg atcacgcccg ccgcggccag cgtgccccag tccatgcccg cggccgagac
     3481 cgtgcgcgtc atcaccgcga cgatcggcag cgcgttgacg ctggtcagcg tgcgcgccat
     3541 gagcagctcg acccagctga acatgaagca gaagaacgcc gccacgccga tgcccgcctt
     3601 gatcagcggc acgaagatcg tcaggaagaa gcgcgggaag gaatacccgt cgatgtaggc
     3661 ggtctcgtcg atctcgcgcg ggatgccgct catgaacccc tccaggatcc acaccgccag
     3721 cgggacgttg aacagcaggt gcgccagcgc caccgcgatg tgcgtgtcca tcagcccgag
     3781 cgtcgtgtag agctggaaga acggcagcag gaagaccgcc ggcggcgtca tgcggttggt
     3841 gagcagccag aagaagacgt gcttgtcgcc gaggaacgag tagcgcgaga acgcgtaggc
     3901 cgccggcagc gccaccagca gcgagatcac cgtgttcatc gcgacgtact cgagcgagtt
     3961 gatgtagccc gagtaccagg cctcgtcggt gaagatcgtg cggtagttgt cccaggtgaa
     4021 gtggcgcggc cagaggctga acgcgccgag gatctcctcg ttggtcttca ccgacatgtt
     4081 caccatccag tacaccggca ggatcgcgaa ggccaggtac aggacgagga acagcgtgcg
     4141 cttcttgaag cggcggtcat ccatgggtgc tctccccttc ggcaacggtg cccacgcgct
     4201 gcatccaggt gtagaggacg aagcacagca gcaggatgat gaggaagtag atcagcgaga
     4261 acgccgcggc cgggccgagg tcgaactggc cgacggcctt ctgcgtgagg tactgcgaca
     4321 ggaaggtcgt cgcgtccccc gggcccccgc ccgtgagcac gaacggctcc gtgtagatca
     4381 tgaagctgtc catgaagcgc agcagcaccg cgatcatcag cacgccgcgc atcttgggca
     4441 actggatgta gcggaagacg gcgagcttgc tggcgccgtc gatgctcgcc gcctggtagt
     4501 aggcgtcggg aatggcgcgc aggccggcat agcacagcag cgcgacgagc ggcgtccagt
     4561 gccagacgtc catggtcagc accgtgaacc aggcgtggcg ctcgttgccg gtgtagtcgt
     4621 agtcgaagcc catcgaggtg agcgccttgc ccagcaggcc gatgtcggtg cggccgaaga
     4681 tctgccagat cgtgccgacc acgttccacg ggatcagcag ggagatcgcc accaccacca
     4741 gcaccgccga ggccttccag ccgcgggccg gcatcgccag cgcgagcgcg atgcccagcg
     4801 ggatctcgac cagcagcacc gacagcgaga acacgagctg gcgcacgagc gcgccgtgca
     4861 ggtcgtcgtc gcgcatgacc tgcttgaacc agtcggtgcc gacgaagacg tggcgctcgg
     4921 gcgagatgat gtcctgcacc gagtagttca ctaccgtcat cagcggcagc accgcggaga
     4981 acgcgacgca gaccaccacc ggcaggatca tcagccaggc cttctggttg acgggcttga
     5041 tcatcgggcg tctccttgcg cggccgcggc ctcggcgggg cggtcgacca gtcgttcgtc
     5101 gcggtagaag caggtgtgcg ggccgacgac ctgcagccac gcgatgccgc cggcggcggg
     5161 cgcggcctcg tgcatcggca ggcgcgcgcg caccagcgcg tcgccctcgg
//
LOCUS       DASVLN010000824         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_312606, whole genome shotgun sequence.
ACCESSION   DASVLN010000824 DASVLN010000000
VERSION     DASVLN010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312606"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VF453_19515"
     CDS             complement(<1..368)
                     /locus_tag="VF453_19515"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7689905.1"
                     /translation="MKPRAWHHDVVTPALLRHARNTYGRAMRRALDEAGFEDLPLNGP
                     YVIGGLALGNRDVPLGQLIRELRISRQSAGQLVDALVARGYLDRSVDAADRRKLNVAL
                     TERGRAAAAAQAAARERVDGA"
     gene            complement(500..>2006)
                     /locus_tag="VF453_19520"
     CDS             complement(500..>2006)
                     /locus_tag="VF453_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7689906.1"
                     /translation="ITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAAAKVKIEIDSK
                     PEVMDRLDRRMIQLKIEREAVRRETDEASQKRLGLIEEEITRLEREYADLEEIWTAEK
                     AAAQGSAHLKEEIDKLRFEIENLKRKGDFNRVAELQYGKLPDLEKRLAEAQATEAGKA
                     KSGSSPQLLRTMVGAEEIAEVVSRATGIPVSKLMQGERDKLLKMEDKLHERVVGQDEA
                     ITAVSDAIRRSRAGLSDPNRPLGSFLFLGPTGVGKTELCKSLAGFLFDSEDHMIRVDM
                     SEFMEKHSVSRLIGAPPGYVGYDEGGTLTEAVRRKPYSVVLLDEVEKAHPDVFNVLLQ
                     VLDDGRLTDGQGRTVDFKNTVIVMTSNLGSHLIMRMAGQPSEDIREAVWAEVKQHFRP
                     EFLNRIDETVVFHALDQANIEAIAKIQLATLESRVAKMDMVLEVSPAALSEIAKVGFD
                     PVFGARPLKRAIQQQIENPVARLILEGRFGPKDVIPVDVADGQFTFGRTVH"
BASE COUNT          276 a          702 c          673 g          355 t
ORIGIN      
        1 gccccatcga cgcgctcgcg cgccgcggcc tgggccgccg ccgcggcgcg gccgcgctct
       61 gtcagggcga cattgagctt gcggcggtcg gcggcatcga cgctgcggtc gaggtagccg
      121 cgcgccacca gcgcgtcgac cagctgtccg gccgactgcc tcgagatgcg caactcccgg
      181 atcagctggc cgagcggcac gtcgcgattg cccagcgcca gccccccgat gacgtagggc
      241 ccgttgaggg gcaggtcctc gaagccggcc tcgtccagcg cacgccgcat cgcgcggccg
      301 tatgtgttgc gtgcatggcg cagcagcgcc ggagtgacga cgtcgtgatg ccaggcgcga
      361 ggtttcatgc catgaaccat actatccaag tcatggatag tcaatttcgt ttcttatttt
      421 ccggcaccct ccaatgaaaa gcggccccag gccatgaggc ccggagccgc cgaagcgagg
      481 cgtcgtcgcc gcgaaccggt cagtgcaccg tccgcccgaa cgtgaactgc ccatccgcca
      541 cgtcgaccgg gatgacgtcc ttcggcccga agcggccctc gaggatgagc cgcgcgaccg
      601 ggttctcgat ctgctgctgg atcgcccgct tgagcggccg cgcgccgaac accgggtcga
      661 agccgacctt ggcgatctcc gacagcgccg ccggtgagac ctcgagcacc atgtccatct
      721 tcgcgacgcg gctctccagc gtggcgagct ggatcttcgc gatcgcctcg atgttggcct
      781 ggtccagggc gtggaacacc accgtctcgt cgatccggtt caggaactcg ggacggaagt
      841 gctgcttgac ctcggcccac accgcctcgc ggatgtcctc cgagggctgg ccggccatcc
      901 gcatgatcag gtgcgagccg aggttgctgg tcatgacgat caccgtgttc ttgaagtcca
      961 cggtgcggcc ctggccgtcg gtcaggcggc cgtcgtcgag cacctgcagc agcacgttga
     1021 agacgtcggg gtgggccttc tcgacctcgt ccagcagcac gacgctgtag ggcttgcggc
     1081 gcacggcctc cgtgagcgtg ccgccctcgt cgtagccgac gtagcccggc ggcgcgccga
     1141 tcaggcggct gaccgagtgc ttctccatga actcgctcat gtcgacgcgg atcatgtggt
     1201 cctcgctgtc gaacaggaag ccggcgagcg acttgcacag ctccgtcttg ccgacgcccg
     1261 tcggcccgag gaacaggaac gagccgagcg gccggttcgg gtccgacagc cccgcgcgcg
     1321 aacggcggat ggcgtcggac acggcggtga tcgcctcgtc ctggccgacc acgcgctcgt
     1381 gcagcttgtc ctccatcttc agcagcttgt cgcgctcgcc ctgcatcagc ttgctgacgg
     1441 ggatgcccgt ggcgcgcgag acgacctcgg cgatctcctc ggcgccgacc atcgtgcgca
     1501 gcagctgcgg cgagctgccg gacttcgcct tgccggcctc ggtggcctgc gcctcggcca
     1561 ggcgcttctc gaggtcgggc agcttgccgt actgcagctc cgcgacccgg ttgaagtcgc
     1621 ccttgcgctt gaggttctcg atctcgaagc gcagcttgtc gatctcctcc ttcaggtgcg
     1681 cggaaccctg ggccgcggcc ttctcggccg tccagatctc ctcgaggtcg gcgtactcgc
     1741 gctccaggcg cgtgatctcc tcctcgatca ggccgaggcg cttctgcgac gcttcgtcgg
     1801 tctcgcggcg cacggcctcg cgctcgatct tgagctggat catgcggcgg tccagcctgt
     1861 ccatgacctc gggcttggag tcgatctcga tcttgacctt ggcggccgcc tcgtcgatga
     1921 ggtcgatcgc cttgtcgggc aggaagcggt cggtgatgta gcggtggctc agctcggccg
     1981 cggcgacgat ggccgggtcg gtgatc
//
LOCUS       DASVLN010000825         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_153877, whole genome shotgun sequence.
ACCESSION   DASVLN010000825 DASVLN010000000
VERSION     DASVLN010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153877"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1134)
                     /locus_tag="VF453_19525"
     CDS             complement(19..1134)
                     /locus_tag="VF453_19525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX7689907.1"
                     /translation="MSSSRVDALFRPLRVKSLSLANRIVMAPMTRTFSPEGVPGEDVA
                     AYYRRRAENEVGLILSEGTVVDRPASRNHPAIPFFHGERALAGWQRVIDGVHAAGGRM
                     GPQIWHVGAVAAAKDYAPAGIESPSGLLAPGTERGGAMTEEDIADTIAAFGRAAGDAK
                     RLGFDTLEIHGAHGYLIDQFFWAGTNTRGGPFGGATLKERARFAAEVLRSARAAVGPD
                     FPIILRLSQWKQQAYAVRLAETPDAMSDWLVPLVEAGADVLHCSQRRFWEPEFPDVDG
                     PEGLNFAGWAKKLTGATTITVGSVGLTGDFFGAFGGESSQPASLDKLVERLERDEFDL
                     VAVGRALLADPAWARKVHAGERESLKDFSPAAFAELA"
     gene            1367..2224
                     /locus_tag="VF453_19530"
     CDS             1367..2224
                     /locus_tag="VF453_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEX7689908.1"
                     /translation="MFNQLGLIGCGLMGGSFALAMKKAGLVRRVVGYSKSPSTVIQAQ
                     KMGVVDQAAESALLAVSGSDIVLIAVPVAATEATFRAIRHLVTPNALFMDVGSTKGDV
                     VEAARRVLKERMPAFVPAHPIAGKEVAGIAHADATLYQDCQVVLTPVAETDMGLIERA
                     HAAWQGIGAHVRTMTPESHDAAFAAVSHLPHLIAFASYLGVAHQAEGERYLELAGPGF
                     RDFTRIAASDPKVWRDILLANRAQVLAQAEQFKTTLFQFESAMREGNADAIETLIREA
                     AQGRGAWKK"
     gene            2221..>2479
                     /locus_tag="VF453_19535"
     CDS             2221..>2479
                     /locus_tag="VF453_19535"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903087.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; catalyzes CMP phosphorylation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HEX7689909.1"
                     /translation="MTYAIPFLDLPPLRSAAGRVRLPGSKSISNRVLLLAGLAEGTTA
                     VHDLLDSDDTRVMLAALEALGCSLRRDGATVHVTGLGGRLRA"
BASE COUNT          347 a          925 c          871 g          336 t
ORIGIN      
        1 aacggcgccg gcgcgcgctc aggccagctc ggcgaacgcg gccggcgaga agtccttcag
       61 cgactcgcgc tcgcccgcgt gcaccttgcg cgcccaggcc ggatcggcca gcagcgcccg
      121 cccgacggcc acgaggtcga actcgtcgcg ctccaggcgc tcgaccagct tgtccaggct
      181 cgccggctgc gaggactcgc cgccgaacgc gccgaagaag tcgcccgtca ggccgaccga
      241 gcccaccgtg atcgtcgtcg cgccggtgag cttcttcgcc cagccggcga agttcaggcc
      301 ctcgggcccg tccacgtccg ggaactccgg ctcccagaag cggcgctgcg agcagtgcag
      361 cacgtccgcg ccggcctcga ccagcggcac gagccagtcc gacatcgcgt ccggcgtctc
      421 ggccaggcgc accgcgtagg cctgctgctt ccactggctc aggcgcagga tgatggggaa
      481 gtccgggccg acggcggcgc gcgcgctgcg cagcacctcg gcggcgaagc gggcgcgctc
      541 cttgagcgtc gcgccgccga acgggccgcc gcgcgtgttg gtgccggccc agaagaactg
      601 gtcgatcagg tagccgtgcg cgccgtggat ctccagcgtg tcgaagccga gccgcttcgc
      661 gtccccggcg gcgcggccga aggcggcgat ggtgtcggcg atgtcctcct ccgtcatcgc
      721 gccgccgcgc tcggtgccgg gcgcgagcag gccggacggg ctttcgatgc ccgcaggcgc
      781 gtagtccttc gcggccgcga ccgcgccgac gtgccagatc tgcgggccca tgcgcccgcc
      841 cgcggcatgg acgccgtcga tcacgcgctg ccagccggcc agcgcgcgct cgccgtggaa
      901 gaacgggatc gcgggatggt tgcgcgaggc cgggcgatcc acgaccgtgc cctccgacag
      961 gatcaggccg acctcgttct cggcgcggcg ccggtagtag gcggcgacgt cctcgccggg
     1021 cacgccttcg ggcgagaagg tgcgcgtcat gggcgccatg acgatgcggt tggccaggga
     1081 cagcgacttg acgcgcaggg gtcggaacag ggcgtcgacg cgggaggaac tcatgggtgg
     1141 tactccttga tataaatgga acgatcgcta tgatatttct cgtgccagcg gcctgcacgc
     1201 acaaggccat tcgtgcgccg gcgtgcggct gaaaccctgt tcgaccgccg ggcgccccac
     1261 gcgcgccgct agaatcgtcc gtttcgtcct gccgcccgcc aaggcggcag cgccggctcc
     1321 ctcgccgcgc gccgaccacg acctccacac ggaaaacgtt ctccagatgt tcaaccagct
     1381 cggcctgatc ggctgcggat tgatgggcgg ctcgttcgcc cttgcgatga agaaggcggg
     1441 gctcgtcagg cgcgtcgtcg gctacagcaa gtcgccgtcg acggtgatcc aggcgcagaa
     1501 gatgggcgtc gtcgaccagg ccgcggagtc cgcgctgctg gcggtctccg gctcggacat
     1561 cgtgctgatc gccgtgccgg tcgccgccac cgaggccacc ttccgcgcga tccgccatct
     1621 cgtcacgccc aacgcgctgt tcatggacgt cggcagcacc aagggcgacg tcgtcgaggc
     1681 cgcgcgccgc gtgctcaagg agcgcatgcc cgccttcgtg cccgcgcatc ccatcgccgg
     1741 caaggaggtc gcgggcatcg cccacgccga cgcgacgctg taccaggact gccaggtcgt
     1801 gctcacgccc gtggccgaga ccgacatggg cctgatcgag cgcgcccacg ccgcctggca
     1861 ggggatcggc gcgcacgtgc gcacgatgac gcccgagtcg cacgacgccg ccttcgccgc
     1921 cgtcagccac ctgccgcacc tgatcgcctt cgcgtcctac ctcggcgtcg cgcaccaggc
     1981 cgagggcgag cgctacctcg agctggccgg cccgggcttc cgcgacttca cgcgcatcgc
     2041 cgcgagcgac ccgaaggtct ggcgcgacat cctgctggcc aatcgcgcgc aggtgctcgc
     2101 gcaggccgag cagttcaaga cgacgctgtt ccagttcgag tcggccatgc gcgagggcaa
     2161 cgcggacgcg atcgagacgc tgatccgcga ggccgcccag ggccgcggcg cctggaagaa
     2221 atgacgtacg cgatcccctt cctcgacctg ccaccgctgc gctcggccgc cggtcgcgtg
     2281 cgcctgccgg gctccaagag catctccaac cgcgtgctgc tgctggccgg cctggccgag
     2341 ggcaccacgg ccgtgcacga cctgctcgac tccgacgaca cccgcgtgat gctcgcggcg
     2401 ctggaggcgc tgggctgcag cctgcggcgc gacggcgcga cggtgcacgt caccgggctc
     2461 ggcgggcgcc tgcgcgcgc
//
LOCUS       DASVLN010000826         3076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_528267, whole genome shotgun sequence.
ACCESSION   DASVLN010000826 DASVLN010000000
VERSION     DASVLN010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528267"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..1699
                     /locus_tag="VF453_19540"
     CDS             437..1699
                     /locus_tag="VF453_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX7689910.1"
                     /translation="MHRRLFLATLLPALPPATAFAGREAASATAVDTGTPVSSQGAVA
                     EMTGITHRPLRFPADHGAHPDSHIEWWYVTGWLRDTGHPSAARPGNADTTATAQADGE
                     AGDTPPPDFGFQATFFRMRTGLAQDSPSRFAARQLVFAHVALTDLSSGRPAHAAALLH
                     DQRAAREGLGIASVPAADGEQVVRLRDWTLARAPGSGADGRSSHLRIEAPCNGFALSL
                     ALDGTQPVLLQGEAGYSRKGPRPGQASRYYSEPQLRVRGHVGRTGAPPREVAGRAWLD
                     HEWSDELMSPDAAGWDWIGIDLLDGGALMAFRMRGRDGRPVWAGGSHRDASGTLRVFG
                     PDEVEFRPLRRWRSPRTQAEYPVEWQLRTPAGRFRLAALHDDQELDSRASTGAVYWEG
                     LSGLHDENGRVVGWGYLELTGYLERLRL"
     gene            2021..2266
                     /locus_tag="VF453_19545"
     CDS             2021..2266
                     /locus_tag="VF453_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689911.1"
                     /translation="MKPQDTSTDLQHIYRQLKRGLGHELVNDENVFALIDLAERDGNP
                     TIATALREWQAPCSDSNDGPRPTMAPTPGFNKLNVKH"
     gene            2644..2997
                     /locus_tag="VF453_19550"
     CDS             2644..2997
                     /locus_tag="VF453_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX7689912.1"
                     /translation="MSVSDELGKLGDLHQRGILSDEEFANAKARVLGGPAARPVPPPS
                     AIQALRRDAYDRWLGGVCGGLGVATGVPSWVWRLIFVFGMLCGGTGLLLYVLLWIFVP
                     PQMPYLPPQDPAQAR"
BASE COUNT          402 a         1192 c         1069 g          413 t
ORIGIN      
        1 tgaaccggcg ctacatgacg ctggaaggcc tgcagaccct cagcgatact ccgcgcactc
       61 ggctgcccgc agtggcgcgc tgagcatgtg atcctcagcc tgttcgggta agcgacttac
      121 accacggctt gggacatcac cgggtcttgc cccacaacaa cccccctcgc agagcgcacg
      181 cttcggggtc agggcgtact cacggcttcg aagtcccttc ttgccagcca gtcaagccaa
      241 gcaaattggc cgaacttcgg tcgcccgagc ggcacgtcga agctgatttg ccgaaatctc
      301 gccgctggcg agctgccttc ggaaacgcct ccgaagcagg cgcggcacgg ctacgatcct
      361 cgttctgctt ggccaagact gctctgcccg acgatttgcg ccgccccgcg gtcaacggcg
      421 tgcgggccgc ctcctgatgc accgccgcct tttcctcgcc acgctgctgc ccgccctgcc
      481 gcccgcgacc gctttcgccg ggcgcgaggc ggcgtccgct accgccgtcg acacgggcac
      541 gccggtgtcg tcgcagggag ccgttgcgga gatgaccggc atcacgcacc gcccgctgcg
      601 cttcccggcc gaccacggcg cccatcccga cagccacatc gagtggtggt acgtcaccgg
      661 ctggctgcgc gacacgggcc acccgagcgc cgcgcgcccc ggcaacgcgg acaccaccgc
      721 cacggcgcaa gccgacggcg aagcggggga cacccccccg ccggacttcg gcttccaggc
      781 caccttcttc cgcatgcgca ccggcctcgc gcaggactcg ccgagccggt tcgccgcgcg
      841 gcagctcgtg ttcgcgcacg tcgcgctgac cgacctctcg tcgggccggc cggcgcatgc
      901 cgcggcgctg ctgcacgacc agcgggccgc gcgcgagggc ctcgggatcg cctcggtgcc
      961 ggccgccgat ggcgagcagg tggtgcggct gcgcgactgg acgctcgccc gtgcgcccgg
     1021 cagcggcgcc gacgggcgct cgtcgcacct gcgcatcgag gccccgtgca acggcttcgc
     1081 gctgtcgctg gcgctggacg ggacccagcc ggtgctgttg cagggcgagg ccggctattc
     1141 gcgcaagggg ccgcggccgg ggcaggcatc gcgctactac agcgagccgc agttgcgggt
     1201 gcggggccac gtcgggcgca ccggtgcgcc gccgcgagag gtagcgggcc gcgcgtggct
     1261 cgaccacgag tggagcgacg agctgatgtc gccggacgcc gccggctggg actggatcgg
     1321 catcgacctg ctcgacggcg gcgcgttgat ggcctttcgc atgcgcggcc gcgatgggcg
     1381 gccggtctgg gccggcggct cgcatcggga cgcgtccggc acgctgcgtg tgttcggccc
     1441 cgacgaggtc gagttccgcc cgctgcgacg ctggcgcagc ccgcgcacgc aggccgagta
     1501 cccggtcgag tggcagctgc ggacgcccgc cggtcgcttt cgcctggccg cgctgcacga
     1561 cgaccaggag ctcgacagcc gcgcgtccac gggcgcggtc tactgggagg gcctgtcggg
     1621 cttgcacgac gagaacggcc gcgtcgtcgg ctggggctat ctggagctga ccggctacct
     1681 ggagcgcctg cgcctctgac ttgacccgca gcggggccgc aggcggcggg cgctgccgac
     1741 atccgtcctg ccggagccca ccggggcgtc gccgcggctg ccgccagcgc gccaagcccg
     1801 acgcccgcat cgatggcgcg aggccacccg cgaccgcacg gccaccgcat gccaccgctt
     1861 ggatcgcctt gggccggccg ggccgatgcc tcgacgctcc caaggacatt acgcgcgcgc
     1921 cccatcggtg ctttactgcc ggggccgccg cgccatcccg cgcgacgcgg gccccgcggc
     1981 acggccgttg ccgcttgcgg atccaccccc tggagagccc atgaaacctc aggacacctc
     2041 gaccgacctg cagcacatct atcgccagct caagcgcgga ctcggccacg agctcgtcaa
     2101 tgacgagaac gtcttcgcgc tgatcgacct cgccgagcgc gacggcaacc cgacgatagc
     2161 caccgcgctg cgcgaatggc aggcgccttg cagcgactcg aacgacggcc cccgcccgac
     2221 catggcgccc acgccaggct tcaacaagtt gaacgtcaag cactgacggc acagaccgtc
     2281 ccggcgtccc gcccctggcg tccggctgcc gcgggccgcc ggcggccgcc tcacggcagg
     2341 cgtccgtcgg aagcgcccgt tggcaggcgc ccgttgcagg cgcctgtccg ttggcaggcg
     2401 actccccgtc cggcgactcc ccggctggca cgccactcct gcgcggcgcc acgatccgat
     2461 gcgcggagtg gcggtcgcgc agcatgccgg ccatgcgggg cgctacggca cgagccggcc
     2521 tgtcgtcgcg atcgaggggc cggtcggccg cccgcgcgat ccgtcgcacg gcactggccg
     2581 ggccgcgccg cggggtctaa cctgcaggca tcgatcactc agggcttcac cacggagacc
     2641 accatgtctg tcagcgacga actcggcaag ctgggcgacc tgcaccagcg cggcatcctc
     2701 tcggacgagg aattcgcgaa cgccaaggcg cgcgtgctgg gcggcccggc cgcacgcccg
     2761 gtgcctcccc cgtccgccat ccaggcactc cggcgcgacg cgtacgaccg ctggctcggc
     2821 ggcgtctgcg gcggcctcgg cgtcgcgacc ggcgtaccct cctgggtctg gcggctgatc
     2881 ttcgtcttcg gcatgctgtg cggcggcacc ggcctgctgc tgtacgtgct gctctggatc
     2941 ttcgtgccgc cgcagatgcc ctacctgccg ccgcaggatc ccgcgcaggc gcgctgagcg
     3001 ctgcccacgg ccgcgcgcgg gcgcccgccg cggggaagac aggcggcgcc gcccgccccg
     3061 cgttgttcgg gcgctc
//
LOCUS       DASVLN010000827         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_153977, whole genome shotgun sequence.
ACCESSION   DASVLN010000827 DASVLN010000000
VERSION     DASVLN010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153977"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VF453_19555"
     CDS             <1..227
                     /locus_tag="VF453_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689913.1"
                     /translation="WATLSARWRPALDARIAELVRLRDQLDACIGCGCLSLKKCALYN
                     PGDAAAAQGPGARFLLAPAATNPGAACGGS"
     gene            256..561
                     /locus_tag="VF453_19560"
     CDS             256..561
                     /locus_tag="VF453_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEX7689914.1"
                     /translation="MRILTWLLRAFLFFVLVAFAINNEQPVTLNWFFSYAWTAPLVLI
                     VLVAFASGAVFGVLAMTPSWWRHRRRARRLEAPAAAPAPAVPAAAPAPDVIVVRDGL"
     gene            551..>1562
                     /locus_tag="VF453_19565"
     CDS             551..>1562
                     /locus_tag="VF453_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7689915.1"
                     /translation="MDFDFQWFLLGLPLFFILGWAGSRFDLRQVRRETRSEPSAYYKG
                     LSLLLNEQQDKAIDAFIETVQNDPDTTELHFALGNLFRRRGEFERSVRVHQHLLVRAD
                     LPQAERERAQHALAQDFVKAGLFDRAEEAYKALEGTAYHTEARLALLNLYERSRDWRA
                     ALETAQQLEAAGTGSFARRISHYWCELALVAEDQHHADEAGRALQHALKADPASARPW
                     VMQGQRLLRAGKPAEALDAWNELRLRDPGSFALVAADYAAAALACDRAPLAKAALEKL
                     LADAPRLDFLAAWAKLEPDAEVVTHRYREMLHQHPTLSAAREVLQQPAIEASARAVGP
                     LAE"
BASE COUNT          194 a          602 c          551 g          215 t
ORIGIN      
        1 actgggcgac gctgtcggcg cgctggcgcc cggcgctgga cgcgcgcatc gcggagctcg
       61 tgcggctgcg cgaccagctc gacgcctgca tcggctgcgg ctgcctgtcg ctgaagaagt
      121 gcgcgctcta caacccgggc gatgcggcgg ccgcgcaggg ccccggcgcg cgcttcctgc
      181 tcgcgcccgc cgcgacgaac ccgggcgcgg cctgcggcgg ctcgtagaat ccgcgcgaac
      241 catcgacacc tcacgatgcg catcctgacc tggctcctgc gggccttcct cttcttcgtg
      301 ctggtcgcct tcgcgatcaa caacgagcag ccggtgaccc tgaactggtt cttctcgtac
      361 gcctggacgg cgccgctggt gctgatcgtg ctggtggcgt tcgcctcggg cgccgtgttc
      421 ggcgtgctcg cgatgacgcc cagctggtgg cgccaccgcc gccgcgcgcg ccgcctcgag
      481 gcgcccgcgg cggcgccggc gcccgccgtt cccgccgctg cgccggctcc cgacgtcatc
      541 gtggtgcgcg atggactttg acttccagtg gttcctgctg ggcctgcccc tgttcttcat
      601 cctaggctgg gcgggctcgc gattcgacct gcgccaggtg cggcgcgaga cccgcagcga
      661 accgtcggcc tactacaagg gcctgagcct gctgctcaac gaacagcagg acaaggcgat
      721 cgacgcgttc atcgagaccg tccagaacga ccccgacacc accgagctgc acttcgcgct
      781 cggcaacctg ttccgccgcc gcggcgagtt cgagcgctcg gtgcgcgtgc accagcacct
      841 gctcgtgcgc gccgacctgc cgcaggccga gcgcgagcgc gcgcagcacg cgctggcgca
      901 ggacttcgtg aaggccggcc tgttcgaccg ggccgaggag gcctacaagg cgctcgaggg
      961 cacggcgtac cacaccgagg cgcggctcgc gctgctgaac ctgtacgagc gctcgcgcga
     1021 ctggcgcgcc gcgctggaga cggcgcagca gctcgaggcc gccggcaccg gctcgttcgc
     1081 ccgccgcatc tcgcattact ggtgcgagct ggcgctcgtc gccgaggacc agcaccacgc
     1141 cgacgaggcg gggcgcgcgc tgcagcatgc gctcaaggcc gaccccgcgt ccgcccgccc
     1201 gtgggtgatg cagggccagc gcctgctgcg cgccggcaag ccggcggaag ccctcgacgc
     1261 ctggaacgag ctgcgcctgc gcgaccccgg gagcttcgcg ctcgtcgccg cggattacgc
     1321 cgccgcggcg ctcgcctgcg accgcgcgcc gctcgccaag gcggcgctcg agaagctgct
     1381 cgccgacgcg ccgcgcctgg acttcctcgc cgcctgggcg aagctcgagc cggacgcgga
     1441 ggtcgtcacg caccgctacc gcgagatgct gcaccagcac ccgaccctgt cggccgcgcg
     1501 cgaggtgctg cagcagccgg ccatcgaggc gtcggcccgg gcggtcgggc cgctggccga
     1561 gc
//
LOCUS       DASVLN010000828         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_528445, whole genome shotgun sequence.
ACCESSION   DASVLN010000828 DASVLN010000000
VERSION     DASVLN010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528445"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VF453_19570"
     CDS             <1..533
                     /locus_tag="VF453_19570"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7689916.1"
                     /translation="ATALLLALAAAALVMAVGIVATSSRTGILGVALLALWGMLDRRL
                     ERRARVALLALPVAVAAAWLSEARWARAHGVAFTLVERVSNDPSSFRFNIWHDALQLI
                     AQNPWTGVGWGNFNFAWTLSPFGFRPEGPTDNAHDLALQLAVEHWRCWSGPCGAASGA
                     RAGSRARTPRRRAARW"
     gene            533..1162
                     /locus_tag="VF453_19575"
     CDS             533..1162
                     /locus_tag="VF453_19575"
                     /inference="COORDINATES: protein motif:HMM:NF023274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HEX7689917.1"
                     /translation="MVLQIGMHSLLEYPLWYVYLLYPAAWAWGLLLGAGARAEPEAIA
                     ADEAARRARAWRMWGALMVTSGLFAYVDYLTVVPLFQPPTKEAGREPAQALPELIVRG
                     QASLLFGTKADFVLATLAPDPSAAMPEIRRAAHALLSPPVLFAWANALARQGQVDKAR
                     YLAERLHEYPIPDGQRFFGVCAEPQVASAPSKPYQCTPSSGHLTWRDFR"
     gene            1287..3101
                     /locus_tag="VF453_19580"
     CDS             1287..3101
                     /locus_tag="VF453_19580"
                     /inference="COORDINATES: protein motif:HMM:NF023274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HEX7689918.1"
                     /translation="MNSSRTTFSDIPGARLAAYAAAIVLPAMCAFSRWQPSPTLINET
                     LALGLWGVCLMLLPATPPAGARRHPASMLLVAMGLLAAAVVGSWTLGSLPTSLALPPL
                     GLILIACAVAMLGARAAQEGGDGARAFAPFAIGMVVAGVLSAIVAVIQVHFGDASWLP
                     DADIIARSGLPGRAVGNLRQPNHLASLLVWAIISLVPLAQWRLVPRGLALALGLFLMY
                     AVVLSGSRTGLVGGALVLALWGAFDSQLSRATRIVLAASLLVPAWIYASGWLGLHWGS
                     GSEVGAARRVTEGDSSHFAIWRNTLAMIAQQPLLGVGWGEFNFAWTLTPFPDRPVAFF
                     DHTHDLFLQFAVELGIPLALVVTGLLVTALLQGMKRAWATAGEAGHGARAAWVMVLMI
                     AVHSLDEYPLWYAYFLLPAAWAWGFALGSGPAPQAEGALGLSGIAGGPRSSDSGATLV
                     SLGSEGTGLRWFGAVTLLVAVFATIDYHRVSTIFSDEGGLPDLKDRISAGETSPFFAH
                     HADYAEATTADPPSRALGALERATHALLDTRLMIAWANALAESGQADRARYIAARLRE
                     FHKEDADEFFAPCDDATIVDKPFQCQPEPKGFTWRDFR"
     gene            3118..>4365
                     /locus_tag="VF453_19585"
     CDS             3118..>4365
                     /locus_tag="VF453_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689919.1"
                     /translation="MNSLKSLSSGSRLTLLDALVCAYIALPLLVFCAWFKWPVAAGLA
                     LLAAYGFVQSLPGVQWRRFEVGRGALAVIVVVSLAWTALAGIGHFFYANLDWVTRDAV
                     LRDLVETPWPPRYDTDGTMSMILRAPVGFYLPVAAVGSLAGLRCADVLLYLWSAFGFG
                     LVLCAMSTLFTTRRERVLGGALMLGFGGLDLVGYFAQSHALPHVGEHIEWWAAFAQYS
                     SNSTLMFWVPNHALPAWLGLMLVLRHWRSRELARMTFMLAASIPLWSPLSAFGLVPFF
                     LVGLNWRRDLGALLAIRSGLPFIALAALVARYMTMDTQAIARGWAIDLFRDAWAFWGR
                     YGLFCLLEFGVLALVLARLRAFDLKVGVAVALLLALPFYHFGPGNDLVMRSSIPALAV
                     LALAAVRPLAAASDRPVARGVLAL"
BASE COUNT          457 a         1609 c         1567 g          732 t
ORIGIN      
        1 gtgccaccgc gctgctgctc gcgctggccg cggcggcgct ggtgatggcg gtgggcatcg
       61 tcgcgaccag ttcgcgcacc ggcatcctcg gcgtcgcgct gctggccctg tggggaatgc
      121 tcgatcgccg gctcgaacgg cgggcccgcg tcgcgctgct cgcgctgccc gtggcggtcg
      181 cggcggcctg gctcagcgag gcgcgatggg cgcgtgcgca tggcgtggcc ttcacgctgg
      241 tcgagcgcgt gagcaacgac ccgtcgtcgt ttcgcttcaa catctggcac gacgcgctgc
      301 agctgatcgc gcagaacccg tggacgggcg tgggctgggg caacttcaac ttcgcgtgga
      361 cgctgtcgcc gttcggcttc cgccccgaag gcccgaccga caacgcgcac gatctcgcgc
      421 tgcagctggc cgtggagcac tggcgctgct ggtcggggcc ttgtggcgcg gcttccggcg
      481 cgcgcgcagg ctcgcgggcg cggacgccgc ggcgacgggc tgcgcgctgg tgatggtgct
      541 gcagatcggc atgcacagcc tgctcgagta cccgctgtgg tacgtctacc tgctgtatcc
      601 cgccgcgtgg gcctggggac tgctgctggg cgccggggcg cgtgccgagc ccgaggcgat
      661 cgccgcggac gaggccgccc ggcgggcgcg tgcatggcgg atgtggggcg cgctgatggt
      721 gacgtcggga ctgttcgcct acgtcgacta cctcaccgtc gtgccgttgt tccagccacc
      781 gaccaaggaa gcggggcggg agccggcgca agcgctgccc gagctcatcg tgcgcggcca
      841 ggccagcctg ctgttcggca ccaaggccga cttcgtgctc gcgacgctcg cgcccgatcc
      901 gtcggcggcg atgccggaga tccggcgcgc ggcgcatgcg ctgctgagtc cgccggtgtt
      961 gttcgcctgg gcgaacgccc tcgcccgcca gggccaggtc gacaaggccc gctacctggc
     1021 cgagcgcctg cacgagtacc cgatccccga cggccagcgc ttcttcggcg tgtgcgccga
     1081 gccgcaggtg gcctcggcgc cctccaaacc ctaccagtgc acgccgtcga gcggccacct
     1141 gacctggcgc gacttcaggt agcggcaatg gcgccgccgg ccccacccgg gaatcgcgtt
     1201 accctacgca ccgcccgccg gcgccgcctc cacccgcacg gcgcaacccc tcgaccgctc
     1261 caccgcccga ccctcgcatc gcccgcatga acagcagccg cacgaccttt tccgacatcc
     1321 cgggggcgcg cctggccgcc tacgcagcgg ccatcgtcct gccggcgatg tgcgccttca
     1381 gccgctggca gccgtcgccg acgctgatca acgagacgct cgccctcggc ctgtggggcg
     1441 tgtgcctgat gctgctgccg gccacgccgc ccgcgggcgc gcggcggcat ccggcgtcga
     1501 tgctgctggt cgcgatgggc ttgctcgcgg ccgcggtggt cggctcgtgg acgctgggat
     1561 cgctgccgac ttcgctcgcg ctgccgccgc tggggctgat cctgatcgcc tgcgcggtgg
     1621 cgatgctggg agcgcgcgcg gcgcaggagg ggggcgacgg ggcgcgcgcg ttcgcgccgt
     1681 tcgcgatcgg catggtcgtc gcgggcgtgc tgagcgccat cgtcgcggtg atccaggtgc
     1741 acttcggcga tgcctcgtgg ctgcccgacg cggacatcat cgcccgctcc ggcctgccgg
     1801 gccgggccgt gggcaacctg cgccagccca accatctcgc gagcctgctg gtgtgggcca
     1861 tcatctcgct cgtgccgctc gcccagtggc gcctggtgcc gcgcggcctg gcgctcgcgc
     1921 tcggcctgtt cctgatgtac gccgtcgtgc tgagcggctc gcgcaccggc ctggtcggcg
     1981 gcgcgctggt gctggcactc tggggcgcct tcgactcgca gctgtcgcgc gcgacgcgca
     2041 tcgtgctggc ggcctcgctg ctggtgccgg cgtggatcta cgcgagcggc tggctgggcc
     2101 tgcattgggg ctcgggctcg gaggtcggcg ccgcccgccg cgtcaccgag ggcgacagct
     2161 cgcacttcgc gatctggcgc aacacgctcg cgatgatcgc gcagcagccg ctgctgggcg
     2221 tcggctgggg cgagttcaat ttcgcatgga cgctcacgcc gttccccgac cggccggtcg
     2281 ccttcttcga ccacacgcac gacctgttcc tgcagttcgc cgtggagctg gggatcccgc
     2341 tggcgctggt cgtgacgggc ctgctggtga ccgcgctgct gcagggcatg aagcgggcct
     2401 gggcgaccgc cggcgaggcc gggcatggcg cgcgcgcggc ctgggtgatg gtgctgatga
     2461 tcgcggtgca cagcctggac gagtacccgc tctggtatgc ctacttcctg ctgccagcgg
     2521 cctgggcctg gggcttcgcg ctgggctcgg ggccggcgcc gcaggcggag ggcgcgctcg
     2581 gcctgagcgg catcgccggc ggtccgcgca gctcggacag cggtgcgacg ctcgtcagcc
     2641 tcggcagcga gggcaccggc ctgcgctggt tcggcgcggt gacgctgctg gtggcggtgt
     2701 tcgcgaccat cgactaccac cgcgtctcga ccatcttctc cgacgaaggt ggactgcccg
     2761 acctcaagga tcgcatcagc gccggcgaga ccagcccctt cttcgcgcac catgccgact
     2821 acgccgaagc cacgaccgcc gacccgccct cgcgcgcgct cggcgccctc gagcgcgcga
     2881 cccacgccct gctcgacacg cggctgatga tcgcctgggc caacgcgctc gccgagagcg
     2941 gccaggccga ccgcgcccgc tacatcgccg cgcgcctgcg cgagttccac aaggaggacg
     3001 ccgacgagtt cttcgcgccc tgcgacgacg cgacgatcgt cgacaagccc ttccagtgcc
     3061 agccggagcc caagggcttc acctggcggg acttccgctg agcccgacgc gacccccatg
     3121 aattccttga agagcctgtc ctccggatcc cgtctcacgc tgctggatgc cctggtctgc
     3181 gcctacatcg cgctgccgct gctggttttc tgcgcctggt tcaagtggcc ggtcgcggcc
     3241 ggcctggcgc tgctggccgc ctacggcttc gtgcagtcgc tgcccggcgt gcagtggcgc
     3301 cgcttcgagg tgggacgggg cgcgctcgcg gtcatcgtcg tcgtgagcct tgcctggacc
     3361 gcgctcgccg gcatcggcca cttcttctac gccaacctgg actgggtcac ccgcgacgcc
     3421 gtgctgcgcg acctggtcga gacgccgtgg ccgccgcgct acgacacgga cggcacgatg
     3481 tcgatgatcc tgcgcgcgcc cgtcggcttc tacctgccgg tcgcggccgt cggctcgctc
     3541 gccgggctgc gctgcgccga tgtcctcctg tacctgtggt cggcgttcgg cttcggcctc
     3601 gtgctgtgcg ccatgtcgac cttgttcacg acccggcgcg agcgcgtgct cggcggcgcc
     3661 ctgatgctcg gcttcggagg cctcgacctc gtgggctact tcgcgcagtc gcacgcgttg
     3721 ccgcatgtcg gcgagcacat cgagtggtgg gccgccttcg cgcagtactc gtcgaactcg
     3781 acgctgatgt tctgggtgcc caaccatgcg ctgcccgcct ggctcgggtt gatgctggtg
     3841 ctgcggcact ggcgctcgcg cgagctggcg cgcatgacct tcatgctcgc ggcctccatc
     3901 ccgctgtggt cgcccttgtc cgcgttcggg ctggtgccgt tcttcctcgt cggactgaac
     3961 tggcgccgcg acctgggcgc cttgctggcg atccgcagcg gcctgccgtt catcgcgctg
     4021 gccgcgctcg tggcgcgcta catgacgatg gacacccagg ccatcgcgcg cggctgggcg
     4081 atcgatctct tccgcgacgc ctgggcgttc tggggccgct acggcctgtt ctgcctgctc
     4141 gagttcggcg tgctcgcgct cgtcctggcg cggctgcgcg ccttcgacct caaggtgggg
     4201 gtcgcggtcg cgctcctgct cgcgctgccc ttctaccatt tcgggccggg caacgacctg
     4261 gtcatgcgca gttccatccc ggcgctggcg gtgctggcgc tggcggcggt gcggccgctc
     4321 gcggcggcgt ccgaccggcc ggtcgcccgc ggcgtgctgg cgctc
//
LOCUS       DASVLN010000829         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_97436, whole genome shotgun sequence.
ACCESSION   DASVLN010000829 DASVLN010000000
VERSION     DASVLN010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97436"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..750)
                     /locus_tag="VF453_19590"
     CDS             complement(94..750)
                     /locus_tag="VF453_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEX7689920.1"
                     /translation="MRPSTFLPSLAALSIALAAAAPARAADDATAGAPTDQVVVPQVE
                     RRDVHVPRYPSNDFELGLFGGAWQTQSFGASGLWGVRGGYDITEDFFVEATYGRTRVS
                     DRTFRQILPGGIFTKAQERLSYYDLSLGWNFLPGEIFIGRNHAYASTLYVIGGVGNTT
                     FDSQRNQTWNFGLGAKLHLADWVALRADVRDHIFSLDLLGKRSSTQNPELTLGVAFFF
                     "
     gene            complement(773..>1603)
                     /locus_tag="VF453_19595"
     CDS             complement(773..>1603)
                     /locus_tag="VF453_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689921.1"
                     /translation="AAASAPGDAVPAPAGPPVARVTTRSGEAIEVDAPLAATHPPEVP
                     RADSVPGGDKVERVQVTDAYVELHTFPGRGYPVFYVAGRGEWLSIELRHTDWFKVRTA
                     SGKEGWVTRAQLETTLTESGGRQTFRDVLLADYLARRVELGAAYGRFHTEPMVKFWLS
                     ARMSDTLSLEWTLAQVQGVFSGTTLWHVNLQAEPWSDQRFSPFAGIGIGRFDNIPNQS
                     LVENQKTNARLADAIVGARWHLTDRFMVRADYAIYTAFVSDQRSLEYRAASLGMSFFF
                     "
BASE COUNT          233 a          517 c          639 g          214 t
ORIGIN      
        1 cggcggcggc tgcgccggcg gcgcgggccg gtgcggcgag ggcgagcgcc agcgagagcg
       61 cggcggcggc ggtgcggagg aggcgggtca tgctcagaag aagaaggcga cgcccagcgt
      121 cagctcgggg ttctgggtgg acgagcgctt gccgagcagg tccagcgaga agatgtggtc
      181 gcgcacgtcg gcgcgcagcg cgacccagtc ggccaggtgc agcttggcgc cgaggccgaa
      241 gttccaggtc tggttgcgct ggctgtcgaa ggtggtgttg cccacgccgc cgatcacgta
      301 cagcgtcgat gcgtaggcgt ggttccggcc gatgaagatc tcgcccggca ggaagttcca
      361 gcccagcgac aggtcgtagt aggacagccg ctcctgcgcc ttggtgaaga tgccgcccgg
      421 caggatctgg cggaaggtgc ggtcgctgac gcgcgtgcgg ccgtaggtgg cctcgacgaa
      481 gaagtcctcg gtgatgtcgt agccgccgcg cacgccccac aggccgctcg cgccgaagct
      541 ctgggtctgc caggcgccgc cgaacagccc gagctcgaag tcgttggacg gatacctggg
      601 cacgtgcacg tcgcggcgct cgacctgcgg cacgacgacc tggtcggtgg gcgcgccggc
      661 ggtggcgtcg tcggccgcgc gtgccggcgc ggcggcggcc agcgcgatcg acagcgcggc
      721 cagcgagggg aggaaggtcg aaggacgcat ggggatcccg gggcgatggg cgtcagaaga
      781 agaacgacat cccgagcgag gccgcgcggt actcgagcga gcgctggtcg gagacgaagg
      841 cggtgtagat cgcgtagtcg gcgcgcacca tgaagcggtc ggtgagatgc cagcgcgcgc
      901 cgacgatcgc gtcggcgagg cgcgcgttgg tcttctggtt ctcgaccagg ctctggttcg
      961 ggatgttgtc gaagcgcccg atgccgatgc ccgcgaacgg ggagaagcgc tggtcgctcc
     1021 agggctcggc ctgcaggttc acgtgccaca gcgtcgtgcc ggagaacacg ccctgcacct
     1081 gcgcgagcgt ccattcgagg ctcagcgtgt ccgacatgcg cgccgacagc cagaacttga
     1141 ccatcggctc ggtgtggaag cggccgtagg cggcgccgag ctcgacgcgg cgggcgaggt
     1201 agtcggccag caggacgtcg cggaaggtct ggcggccgcc ggactcggtc agcgtcgtct
     1261 cgagctgcgc gcgcgtcacc cagccttcct tgccggaggc cgtgcgcacc ttgaaccagt
     1321 cggtgtggcg cagctcgatc gacagccact cgccgcggcc ggcgacgtag aagaccgggt
     1381 agccgcggcc cgggaaggtg tgcagctcga cgtaggcgtc ggtcacctgc acgcgttcga
     1441 ccttgtcgcc ccccggcacc gagtcggccc ggggcacctc gggcggatgc gtggccgcga
     1501 gcggggcgtc gacctcgatc gcctcgcccg agcgcgtggt cacgcgggcg accggcggcc
     1561 cggcgggcgc cgggacggca tcgccgggcg ccgatgcggc ggc
//
LOCUS       DASVLN010000830         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_154139, whole genome shotgun sequence.
ACCESSION   DASVLN010000830 DASVLN010000000
VERSION     DASVLN010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154139"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VF453_19600"
     CDS             complement(<1..1322)
                     /locus_tag="VF453_19600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7689922.1"
                     /translation="MDATTAPQPAARADEPPAPGLLRLAWPIFAEQSLHMLTGIVDTL
                     MASRIGDDAVGGLGSAWQIVVIFLMGFNVLSVGASVATTHHLGLGDRGGARRLARGAV
                     ATNLWVGAAFSVVLATTAPTTLRWAQLAPAQLAYALPFLRWMGGTLFLESMNFALCAI
                     LRANGHTREVMFVILAQNVVNAGASATLIFGLCGLPALGVTGIAIATSISRLLACAAL
                     WVLARRHVALTFRPRDLVDIRLADLRRLLGFGLPAVLENVSWYAAFMVVTAFTARMGG
                     RALATQAYVQQVASIEMLASLSIGIAGEIVVGRLVGAGRLDEAKARGLAHVRIGLLVT
                     TGVAAVAAVLAPPALRVFTHDPDIVATGRILVAMGLLLEPGRALNLIVIAALRASGDT
                     RFPLVAGLASQWLLMACGAWLLGTALHLGLVGVWCALILDEGLRGLAML"
     gene            1505..2440
                     /locus_tag="VF453_19605"
     CDS             1505..2440
                     /locus_tag="VF453_19605"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020610563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7689923.1"
                     /translation="MTAPALAPRPYRGVFPVVPTIFDEQGGLDLAGQKRCVDFMIDAG
                     SNGLCILANFSEQFALADDERETLTRTILEHVAGRVPVIVTTTHFSTDVCLERSRRAQ
                     AQGAAMVMVMPPYHGATLRVGDKQLERFFGRLSDGLAIPIMIQDAPMAGTPLAPAFLA
                     RLAREHERIRYFKIETAGAANKLRELIALGGDAIEGPWDGEEAITLLADLDAGATGAM
                     TGGGYPDGIRTIVDPFLAGDREAAVAAYGRWLPLINFENRQGGFLAAKALMQAGGIIA
                     CDAPRCPFPAMHPATREGLLDAARRLDPLVLRWSK"
     gene            2507..3943
                     /locus_tag="VF453_19610"
     CDS             2507..3943
                     /locus_tag="VF453_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7689924.1"
                     /translation="MTRFDNLIAGEWTPGVTTRPNVNPSDTADIIGDYAQADAAQALR
                     AIEAAQAAAPAWAASTPQQRFDVLDAVGSELLARKDEIGRLLSREEGKTLPEGIGETV
                     RAAMIFKFFAGEALRTGGESVASVRPGVGVEMLRQPVGVVGLITPWNFPIAIPAWKIA
                     PALAYGNCVVFKPAELVPGCGWTLAEILSRTSLPKGVFNLVMGAGSQVGQALTDSAAV
                     RAISFTGSVGVGRRLIAAAAARQAKVQAEMGGKNPLVVLDDADLAVAVNCAVQGAYFS
                     TGQRCTASSRFIVTAGIHDAFVDGVLAKLKSLKVDNALLPGTDIGPVVDERQLAQNLQ
                     YVDIAKAEGGELVFGGERLERATPGFYQQPALFTGTTNAMRISREEVFGPVANVVKVQ
                     DFDEALAVANDTEFGLSAGICTRSLKAATQFKLLSQAGMVMVNLPTAGVDYHVPFGGT
                     KGSSYGPREQGAYAREFYTTVKTAYIGA"
BASE COUNT          612 a         1494 c         1430 g          513 t
ORIGIN      
        1 agcatggcca ggccgcgcag gccctcgtcg aggatcaggg cacaccacac gcccaccagg
       61 ccgaggtgca gcgcggtgcc cagcagccag gcgccgcagg ccatcagcag ccactgcgac
      121 gcgaggccgg cgacgagcgg gaagcgcgtg tcgccgctcg cgcgcagggc ggcgatgacg
      181 atcaggttga gcgcgcgccc cggctccagc agcaggccca tcgcgacgag gatgcggccg
      241 gtggcgacga tgtcgggatc gtgcgtgaag acgcgcagcg ccggcggcgc gagcaccgcg
      301 gcgaccgcgg ccacgcccgt cgtgaccagc aggccgatgc gcacgtgcgc gaggccgcgc
      361 gccttcgcct cgtcgagccg ccccgcgccg accagccggc cgacgacgat ctcgccggcg
      421 atgccgatgg acaggctcgc gagcatctcg atcgacgcga cctgctgcac gtaggcctgc
      481 gtcgcgagcg cccggccgcc catgcgcgcg gtgaaggccg tgacgaccat gaacgcggcg
      541 taccagctca cgttctccag cacggcgggc aggccgaagc cgagcaggcg gcgcaggtcg
      601 gccaggcgga tgtcgacgag gtcgcgcgga cggaaggtca gcgcgacatg ccgccgcgcc
      661 agcacccaca gcgcggcgca ggcgagcagc cggctgatcg aggtcgcgat cgcgatgccc
      721 gtcacgccca gcgccggcag gccgcacagg ccgaagatca gcgtcgcgct cgcgccggcg
      781 ttgacgacgt tctgcgcgag gatcacgaac atcacctcgc gcgtgtggcc gttggcgcgc
      841 aggatggcgc acagcgcgaa gttcatcgac tcgaggaaca gcgtcccgcc catccagcgc
      901 aggaagggca gggcgtaggc gagttgcgcc ggcgcgagct gggcccagcg cagcgtcgtc
      961 ggcgcggtcg tcgcgagcac cacgctgaac gccgcgccca cccacaggtt cgtggcgacc
     1021 gccccgcgcg cgagccggcg cgcgccgccg cggtccccga ggcccaggtg gtgcgtcgtc
     1081 gcgaccgatg cgccgaccga gagcacgttg aagcccatca ggaagatcac gacgatctgc
     1141 cacgcgctgc ccagtccgcc caccgcgtcg tcgccgatcc ggctggccat cagcgtgtcg
     1201 acgatgccgg tgagcatgtg cagcgactgc tcggcgaaga tcggccatgc cagccggagc
     1261 aggccgggcg cgggcggttc gtcggcgcgc gcggcaggct gcggggcggt cgtggcatcc
     1321 atggggcggc aagcttagcg gcgattggtt acggcggatt acggcgcacg caggatgtcg
     1381 catgcacgcc ataaagtatg actattgatc gataaccgcc cgtgatcgca aggagaatgc
     1441 gatttgactc gcagaaaaag tcatacttta atacgcggtc tgccttcgat cgcgagtacc
     1501 gcccatgacc gctcccgccc tcgcgccgcg cccgtaccgc ggcgtcttcc ccgtcgtccc
     1561 gaccatcttc gacgagcagg gcggcctcga cctcgccggc cagaagcgct gcgtggactt
     1621 catgatcgac gccggctcca acgggctgtg catcctcgcg aacttctccg agcagttcgc
     1681 gctggcggac gacgagcgcg agacgctcac gcgcaccatc ctcgagcacg tcgccggccg
     1741 cgtgcccgtg atcgtgacga cgacgcactt ctcgaccgac gtctgcctcg agcgctcgcg
     1801 gcgcgcgcag gcgcagggcg cggcgatggt gatggtcatg ccgccctacc acggcgcgac
     1861 gctgcgcgtg ggcgacaagc agctcgagcg cttcttcggc cgcctgagcg acgggctcgc
     1921 catcccgatc atgatccagg acgcgccgat ggccggcacg ccgctcgcgc cggccttcct
     1981 cgccaggctc gcgcgcgagc atgagcgcat ccgctacttc aagatcgaga cggccggcgc
     2041 cgcgaacaag ctgcgcgagc tgatcgcgct gggcggcgac gcgatcgagg gcccgtggga
     2101 cggcgaggag gcgatcacgc tgctggccga cctcgacgcc ggcgccaccg gcgcgatgac
     2161 cggcggcggc tatcccgatg gcatccgcac gatcgtcgac cccttcctgg cgggcgaccg
     2221 cgaggcggcc gtcgcggcct acgggcgctg gctgccgctg atcaacttcg agaaccgcca
     2281 gggcggcttc ctcgccgcca aggcgctgat gcaggcgggc ggcatcatcg cctgcgacgc
     2341 gccgcgctgc ccgttcccgg cgatgcatcc ggccacgcgc gaagggctgc tcgacgccgc
     2401 gcgccggctc gatccgctcg tgctgcgctg gtcgaagtag gccggccgat ccttttcgct
     2461 tgggcgacac tgcgcgccac ccacccaacc cccccgagga cagagcatga cccgcttcga
     2521 caacctcatc gccggcgagt ggacgccggg cgtcacgacg cgcccgaacg tcaacccgtc
     2581 cgacaccgcc gacatcatcg gcgactacgc ccaggccgac gccgcgcagg cgctgcgcgc
     2641 catcgaggcc gctcaggccg ccgcgcccgc gtgggccgcg tcgacgccgc agcagcgctt
     2701 cgacgtgctc gacgccgtcg gctccgagct gctcgcgcgc aaggacgaga tcggccgcct
     2761 gctctcgcgc gaggaaggca agacgctgcc cgagggcatc ggcgagacgg tgcgcgccgc
     2821 gatgatcttc aagttcttcg ccggcgaggc gctgcgaacg ggcggcgagt cggtcgcctc
     2881 cgtgcggccg ggcgtgggcg tcgagatgct gcgccagccc gtcggcgtgg tcggcctgat
     2941 cacgccgtgg aacttcccga tcgcgatccc ggcgtggaag atcgcgcccg cgctcgccta
     3001 cggcaactgc gtcgtcttca agccggccga gctggtgccg ggctgcgggt ggacgctcgc
     3061 cgagatcctc agccgcacat cgctgcccaa gggcgtgttc aacctggtga tgggggcggg
     3121 ctcgcaggtc ggccaggcgc tgaccgactc ggccgcggtg cgcgccatca gcttcaccgg
     3181 ctcggtgggc gtgggccggc gcctgatcgc cgccgcggcc gcccgccagg ccaaggtgca
     3241 ggccgagatg ggcggcaaga acccgctggt cgtgctggac gacgccgatc tcgccgtcgc
     3301 ggtcaactgc gcggtccagg gcgcctactt ctcgaccggc cagcgctgca ccgcctccag
     3361 ccgcttcatc gtcaccgccg gcatccacga cgccttcgtc gacggcgtgc tcgccaagct
     3421 gaagtcgctc aaggtcgaca acgcgctgct gccgggcacg gacatcggcc cggtcgtcga
     3481 cgagcgccag ctcgcgcaga acctgcagta cgtcgacatc gcgaaggccg agggcgggga
     3541 gctcgtcttc ggcggcgagc gcctggaacg cgcgacgccc ggcttctacc agcagcccgc
     3601 gctgttcacc ggcaccacca acgcgatgcg catcagccgc gaggaggtct tcgggccggt
     3661 cgccaacgtc gtgaaggtgc aggacttcga cgaggcgctc gcggtggcca acgacaccga
     3721 gttcggcctg tccgcgggga tctgcacgcg ctcgctcaag gccgcgacgc agttcaagct
     3781 gctgtcgcag gccggcatgg tgatggtcaa cctgcccacc gccggcgtcg actaccacgt
     3841 gcccttcggc ggcaccaagg gctccagcta cggcccgcgc gagcagggcg cgtacgcgcg
     3901 cgagttctac acgacggtca agaccgccta catcggcgcc tgaagcccgt cgagccgatg
     3961 agcacgagcc ggtcgcgagc ccgcgccgcg gcgccccggg catcctcgac cgccgccggc
     4021 tgcgacccgg ccgcggcccg gccgtgggg
//
LOCUS       DASVLN010000831         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_41863, whole genome shotgun sequence.
ACCESSION   DASVLN010000831 DASVLN010000000
VERSION     DASVLN010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41863"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VF453_19615"
     CDS             <1..277
                     /locus_tag="VF453_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689925.1"
                     /translation="ASAMHPTDEHLLPWFIAAGAGGLDEAPVRLHESVEGGHLGMDAY
                     AFGPEAAALAAAVETASTASSSAGGQRHRELDYIQASAASTTGVAPP"
     gene            complement(229..912)
                     /locus_tag="VF453_19620"
     CDS             complement(229..912)
                     /locus_tag="VF453_19620"
                     /inference="COORDINATES: protein motif:HMM:NF025594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HEX7689926.1"
                     /translation="MLLGFGPSLFIANGPPVSFYGFPYPTRMAVVQLASGALWVWSPV
                     ALTDELAAEVAALGPVRYIVSPNRLHHLFLSAWARRWPEARLYAPPGLAAKRPDLHFH
                     GELGDTPDPAWAQDIDQAVFRGSFALTEVVFFHARSRTAIVGDLIQRFDTSTLSAWKG
                     LLLKLDGLAGEHGSTPRELRITFLRRGAARAARDKVLGWQPEQLLIAHGRCVQGGATP
                     VVDAALAWM"
     gene            997..1647
                     /locus_tag="VF453_19625"
     CDS             997..1647
                     /locus_tag="VF453_19625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7689927.1"
                     /translation="MQTIVPHLKPMQAPAPLARLEITTERLRLLAGDAGIAEAVLAFQ
                     RRNRRHFARWDPPTADAFFTLESQALRVQQGLAAFHADTAYRWWLVDATRAGGARMPT
                     SDVEVIGSIHFSQIARGAFQNAMLGYALDEAYVGRGLMHEALTAGIAEMFSPRVNLHR
                     VQAAYRPENRRSGSVLERLGFRVEGLAPDYLFIEGAWRDHHVAALRNPDFVLPDGW"
     gene            complement(2077..3138)
                     /locus_tag="VF453_19630"
     CDS             complement(2077..3138)
                     /locus_tag="VF453_19630"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX7689928.1"
                     /translation="MSTTPAPGDAAAATRRSQLTALLCAAPKAELHIHIEGSLEPELI
                     FALARRNGVALPYPNVDALRSAYAFTDLQSFLDIYYAGASVLLHEADFADMAWAYFER
                     AAADNVVHCEVFFDPQTHTARGVEIGVVIRGLDAAVQRARAELGLSVRLILCFLRHLS
                     EDDALATLEAALPHRDKFIGVGLDSSERGHPPEKFERVFARARALGLHLVAHAGEEGP
                     PAYIRSALDVLHVERIDHGVRCVEDPALVQRLAAARTPLTVCPLSNVKLRVFDDLREH
                     NVPALLAAGLCVTINSDDPAYFGGYLNENLVQLFDALPELGEDEAYQLLRNSLEASFV
                     DEADKAMWIDELDAVFDAA"
BASE COUNT          513 a         1266 c         1301 g          485 t
ORIGIN      
        1 cgcgtccgcg atgcacccga ccgacgagca cctgctgccg tggttcatcg ccgcgggcgc
       61 cggcggcctg gacgaggcgc cggtgcgcct gcacgagagc gtcgagggcg gccacctggg
      121 catggacgcc tacgcgttcg gcccggaggc ggcggcgctg gccgcggcgg tcgagacggc
      181 gtcgaccgcc tcgtcatcgg ccggcggcca gcggcatcgc gaactcgact acatccaggc
      241 gagcgcggcg tcgacgaccg gtgtcgcgcc accttgaacg cagcggccat gggcgatcag
      301 cagctgctcg ggctgccagc ccagcacctt gtcgcgcgcg gcgcgtgccg ccccgcggcg
      361 caggaaggtg atgcgcaact cgcgtggcgt gctcccgtgc tcgcctgcca gcccgtcgag
      421 cttcagcagc aggcccttcc aggcggagag cgtggaggtg tcgaaccgct ggatcaggtc
      481 gccgacgatc gcggtgcgcg acctggcgtg gaagaacacg acctcggtca atgcgaacga
      541 gccacgaaac accgcctggt cgatgtcctg cgcccacgcg ggatccggcg tatcgcccag
      601 ctccccatgg aagtggaggt ccggccgctt tgccgccagg cccggcggcg catacaagcg
      661 tgcctcaggc caccgccggg cccaggccga caggaacagg tggtgcagcc tgttgggcga
      721 gacgatgtat cgcaccggcc cgagcgccgc cacctctgca gccagttcgt ccgtcagcgc
      781 caccggcgac cagacccaca atgcgcccga cgccagctgg accacggcca tgcgcgtggg
      841 gtacggaaaa ccgtagaacg acacgggtgg accgttggcg atgaacagcg aggggccgaa
      901 cccgagcagc atgcagcgac gatacaccca ggccgatggg acaggagcac ggccagggcc
      961 cgggccgtcc cgcggcccgg gtacgatgtc cgtcccttgc agaccatcgt gccccacctg
     1021 aagcccatgc aggcgcccgc ccccctcgcc cgcctcgaga tcacgaccga gcgcctgcgc
     1081 ctgctcgccg gcgacgccgg catcgcggag gcggtgctcg cgttccaacg ccgcaaccgg
     1141 cgccatttcg cgcgctggga tccgcccacg gcggacgcct tcttcacgct ggagtcgcag
     1201 gcgctgcgcg tgcagcaggg cctggccgcc ttccatgccg acaccgccta ccgctggtgg
     1261 ctggtcgacg ccacccgcgc gggcggcgcg cggatgccga cctcggatgt cgaggtgatc
     1321 ggcagcatcc acttcagcca gatcgcgcgc ggcgcgttcc agaacgcgat gctgggctac
     1381 gcgctggacg aggcctacgt cggccgcggg ctgatgcacg aggcgttgac cgccggcatc
     1441 gcggagatgt tctcgccgcg cgtgaacctg caccgggtgc aggccgccta ccggcccgag
     1501 aaccggcgca gcggcagcgt gctcgagcgc ctgggcttcc gcgtcgaggg cctcgcgccc
     1561 gactacctgt tcatcgaagg ggcctggcgc gaccaccacg tggcggcgct gcgcaacccg
     1621 gatttcgtgc tgcccgacgg ctggtgagcc ggccgcagca cggccggcgt ccggccatcg
     1681 cctccgggct ccacgttacg tggccgggcc gccgacgtcc gaaggccggt ggatcgtccg
     1741 ggagcaccgg gcgtggtccc cggagctccg acgactcgtg aaaatggctc gaccgaggct
     1801 cccgcagccg gggcgccacg gcttcgagcg ccatggatgc acccgttcga ggcatcgatg
     1861 gcgcttcgac gctccggcat cctggccctg ggcgaggacg tcgcgccatt tcgaggcatc
     1921 gttcctgctc cggggccgat cgatgcatct tggggagccg gcgtcggttc ccggagttcg
     1981 agggcccggg cttcgcgaac catcgtcgaa tctccggcac cgatcgccgg ctctcccgga
     2041 gccgtcgtcc ggtcttcgcg gccgggggcc gcgccttcag gccgcgtcga agaccgcgtc
     2101 gagctcgtcg atccacatgg ccttgtcggc ctcgtcgacg aacgaggcct cgaggctgtt
     2161 gcgcagcagc tggtaggcct cgtcctcgcc gagctccggg agcgcgtcga agagctgcac
     2221 gaggttctcg ttgaggtagc ccccgaagta ggccgggtcg tcggagttga tggtcacgca
     2281 caggccggcg gccagcagcg cggggacgtt gtgctcgcgc aggtcgtcga acacgcgcag
     2341 cttgacgttg gacagcgggc acaccgtcag gggcgtgcgc gccgcggcca ggcgctgcac
     2401 gagtgccgga tcctcgacgc agcgcacgcc gtggtcgatg cgctcgacgt gcagcacgtc
     2461 cagcgccgag cggatgtagg ccggcggccc ttcctcgccg gcgtgggcga cgaggtgcag
     2521 gccgagcgcc cgggcgcgcg cgaacacgcg ctcgaacttc tcgggcggat ggccgcgctc
     2581 gctcgaatcc aggccgacgc cgatgaactt gtcgcgatgg ggaagagcgg cctccagcgt
     2641 cgcgagcgcg tcgtcctcgc tcaggtggcg caggaagcac aggatgagcc gcacgctcag
     2701 acccagctcg gcacgggcgc gctgcaccgc ggcatccagg ccgcggatca cgaccccgat
     2761 ctccacgccg cgcgccgtgt gggtctgcgg gtcgaagaag acctcgcagt ggacgacgtt
     2821 gtcggccgcg gcgcgctcga agtaggccca ggccatgtcg gcgaagtcgg cctcgtgcag
     2881 cagcacgctc gcgccggcgt agtagatgtc gaggaagctc tgcaggtcgg tgaacgcgta
     2941 ggccgagcgc agggcgtcca cgttcgggta gggcaacgcg acgccgttgc gccgggcgag
     3001 cgcgaagatc agctcgggct cgagggagcc ctcgatgtgg atgtgcagct cggccttggg
     3061 ggcggcgcac aggagcgcgg tcagctggct gcggcgggtg gcagccgcgg cgtcgccggg
     3121 ggcgggggtg gtgctcatga cgcggggtgg gggcttgtcc ggatgcgccg actgtacgcg
     3181 gggtggcgcg cgccgggacc ctgcgcgccg gcgaatccgg gttccggctt ccgcctgggg
     3241 tggcggtcgc atgcgcgcgg gggcgtcgac cctgccgcgc ttgcccggcg atcacggggg
     3301 gatccgctgc gctgccgccc ttgccgcagt ccggttcgtc gcgcgggtct gcgtttcgtc
     3361 gggtcgtcgc ggcaagtcgt cgcaagtcgc tcgccggtcg acgggcgaag gacgaaagtc
     3421 cagccatcgg atcacgaaac gcgatccagg ccagacaagc aacgcacaca ggagcccatc
     3481 atgaccaaga ccatcgcctc gcaagccgtc gccgccgtcc tcgccgtcgc cgccaccttc
     3541 gccaccgcgt ccggagtgac ccaca
//
LOCUS       DASVLN010000832         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_97692, whole genome shotgun sequence.
ACCESSION   DASVLN010000832 DASVLN010000000
VERSION     DASVLN010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97692"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..>3012)
                     /locus_tag="VF453_19635"
     CDS             complement(100..>3012)
                     /locus_tag="VF453_19635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7689929.1"
                     /translation="AAAGGDTRAPAPVRPRTALPSPARAFGGPEDRDDPYATLAVQAL
                     CLLVTIVGGLLVLLSLAQRAFGPLTLLAESRAMMGTILLVGGGAACVAGRHGRRRLSA
                     TLALLLALAAILLHAWHSGLGTHSIVLGGAVLLVAAGGALVDMPTAVAIAALTMAAVA
                     WMEVAERHGVLSRAVGLAELDAETRSASYAMLLAAGLVAAGVVRRQLLGTLRRGQEQE
                     TRMSRLLQLGSDFTWEMDGRARVTWVSPSFETHTGRRAAEFMRLGEPDGPAVVADAQW
                     ELLQDDLRARRPYRERLIHVVMRDGTELFLCGSGEPVFDAAGRHTGWWGVSRNATAEV
                     LSQRELQRSQTMLDRLVRLSPDAICVASLIDGRVLLANPAFLAFTGLDEGEAMGRNGV
                     ELGLWRDRDQMLDLGRAIAERGSVRDFRSVAWSRDGQQHTVMINAAAFDWDGEPVCVM
                     TTRDITETERARLEGDAILDNAVVGVCLVRQHRLERVNPQLERMLGLEPGSLVGKPTE
                     VLFPSREKFREFVGVYEDAQAAGETIDIERRLTRPDGSRMLIRMRGRAVDPKRRQETG
                     TIWVIEDITERRRAETELAEAKLEAEAASRAKSSFLATMSHEIRTPLSGVLGLARLLQ
                     DASLTPERRTEYLGHLVDAAELLTGIVSDVLDLSKIEAGHLQVEHIPFDLHAVVSSAF
                     RTFSPLGAERGLEMRCEVEPDAPREVVGDPVRVRQILSNYLSNALKFTYRGGIELRLL
                     RRGPDIARIEVRDTGIGVRPDQLETLFRPFTQADSSTTRRFGGSGLGLSICHELAVLM
                     GGRVGLDSDGRTGSCAWVELPLEDAEDAELSTIAGTLDEPPPQPLRGMRVLLAEDNPV
                     NRLIVGAMLDRLGAEVVEAANGAEAIRLATQPARALHAVLMDLHMPEIDGIEATRRLR
                     SQAATAHLPIIALTAAVLDAERAQAQSAGMNAFVTKPAAEPELVRALWPFAPGAAH"
BASE COUNT          397 a         1103 c         1130 g          382 t
ORIGIN      
        1 gggtgcgcgt gacgcgcgcg ggcggcgagg tcgacgcgct cgccccgggc ggcccgatcc
       61 gcatcgggcg cggcgtacaa cgctgatggc gacgtcgccc tagtgcgccg ctcccggcgc
      121 gaagggccac agcgcgcgca ccagctccgg ctcggcggcc ggcttggtga cgaaggcgtt
      181 catgccggcg gactgggcct gggcgcgttc cgcgtcgagc accgcggccg tgagggcgat
      241 gatgggcagg tgggccgtcg cggcctggct gcgcagtcga cgggtggcct cgatgccgtc
      301 gatctcgggc atgtgcaggt ccatcagcac ggcgtgcagc gcgcgcgccg gctgcgtggc
      361 aaggcggatc gcctccgcgc cgttggcggc ttcgacgacc tcggccccga ggcgatcgag
      421 catcgcgccg acgatgagcc ggttgaccgg gttgtcctcg gcaagcagca cgcgcatgcc
      481 gcgcagcggc tgcggcggcg gctcgtcgag cgtgccggcg atggtggaca gctcggcatc
      541 ctcggcatcc tccagcggca gctcgaccca ggcgcagctg cccgtcctgc cgtcgctgtc
      601 caggccgacc cggccgccca tcagcaccgc gagctcgtgg cagatcgaca gcccgagccc
      661 gctgccgccg aagcgccgcg tcgtcgagct gtcggcctgc gtgaacggcc ggaacagggt
      721 ctcgagctgg tcgggccgca cgccgatgcc ggtgtcgcgc acctcgatgc gcgcgatgtc
      781 ggggccgcgc cgcagcaggc gcagctcgat gccgccgcgg taggtgaact tcagcgcgtt
      841 ggacaggtag ttcgacagga tctgccgcac ccgcaccggg tcgccgacga cctcgcgcgg
      901 tgcgtcgggc tccacctcgc agcgcatctc gagcccgcgc tcggcgccca gcggcgagaa
      961 ggtgcggaac gcgctcgaga cgacggcatg gaggtcgaac gggatgtgct cgacctgcag
     1021 gtggccggcc tcgatcttgg acaggtcgag cacgtccgag acgatgccgg tcagcagttc
     1081 cgcggcgtcg acgagatggc ccaggtactc ggtgcggcgc tccggcgtca gcgacgcgtc
     1141 ctgcagcagg cgcgcgaggc ccagcacgcc gctcaggggc gtgcggatct cgtggctcat
     1201 cgtcgcgagg aagctgctct tggcgcggct cgcggcctcg gcctcgagct tggcctcggc
     1261 aagctcggtc tcggcgcggc ggcgctcggt gatgtcctcg atgacccaga tcgtgccggt
     1321 ctcctggcgc cgcttcgggt cgacggcgcg gccgcgcatg cggatcagca tccggctgcc
     1381 gtcggggcgc gtgaggcggc gctcgatgtc gatggtctcg ccggcggcct gcgcgtcctc
     1441 gtagacgccg acgaactcgc ggaacttctc gcgcgacggg aacagcacct ccgtcggctt
     1501 gcccaccagc gagccgggct cgaggccgag catgcgctcc agctgcgggt tgacgcgctc
     1561 gagccggtgc tggcgcacca ggcacacgcc gacgaccgcg ttgtccagga tcgcgtcgcc
     1621 ctcgagccgg gcgcgctcgg tctcggtgat gtcgcgcgtg gtcatcacgc acaccggctc
     1681 gccgtcccag tcgaaggccg cggcgttgat catcacggtg tgctgctggc cgtcgcgcga
     1741 ccaggcgacc gagcggaaat cgcgcacgga gccgcgctcg gcgatcgcgc ggccgaggtc
     1801 cagcatctgg tcgcgatcgc gccagagccc cagctcgacg ccgttgcggc ccatcgcctc
     1861 gccctcgtcc aggccggtga acgcgaggaa ggccggattg gcgagcagca cgcgcccgtc
     1921 gatcaggctg gccacgcaga tcgcgtccgg gctcaggcgc acgaggcggt cgagcatggt
     1981 ctgcgagcgt tgcagctcgc gctgggacag cacctccgcg gtcgcgttgc ggctgacgcc
     2041 ccaccagcca gtgtggcggc cggcggcgtc gaacacgggc tcgccgctgc cgcacaggaa
     2101 cagctcggtg ccgtcgcgca tgacgacgtg gatcagccgc tcgcggtagg gccggcgggc
     2161 gcgcaggtcg tcctgcagca gctcccattg cgcgtccgcg acgacggcgg gaccgtcggg
     2221 ctcgcccagg cgcatgaact cggcggcgcg gcgtcccgtg tgggtctcga acgagggcga
     2281 cacccaggtc acgcgggcgc ggccgtccat ctcccaggtg aagtcgctgc ccagctgcag
     2341 caggcggctc atgcgcgtct cctgctcctg gccgcggcgc agcgtgccga gcagctgccg
     2401 ccgcaccacg ccggccgcga ccaggccggc ggccagcagc atcgcgtacg acgccgagcg
     2461 cgtctcggcg tcgagctcgg ccaggccgac cgcgcgcgac aggacgccgt ggcgctcggc
     2521 gacctccatc cacgcgaccg cggccatcgt gagcgccgcg atcgcgacgg cggtgggcat
     2581 gtccacgagc gccccgcccg cggccaccag cagcacggcg ccgccgagca cgatcgaatg
     2641 cgttcccagc cccgagtgcc aggcatgcag caggatcgcc gccagggcga gcagcagcgc
     2701 gagcgtcgcg gacagccgcc gccggccgtg ccggccggcc acgcaggccg caccgccgcc
     2761 gaccagcagg atcgtgccca tcatggcgcg cgactcggcc agcagcgtca gcggtccgaa
     2821 ggcgcgctgc gcgagcgaca gcagcacgag cagcccgccc acgatcgtca cgagcaggca
     2881 aagcgcctgc acggcgagcg tcgcgtaggg gtcgtcgcga tcctcggggc cgccgaacgc
     2941 gcgggcaggg ctgggcagcg ccgtgcgcgg acgcacgggc gccggtgcgc gggtgtctcc
     3001 tcctgcggcg gc
//
LOCUS       DASVLN010000833         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_42083, whole genome shotgun sequence.
ACCESSION   DASVLN010000833 DASVLN010000000
VERSION     DASVLN010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42083"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1713)
                     /locus_tag="VF453_19640"
     CDS             complement(217..1713)
                     /locus_tag="VF453_19640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HEX7689930.1"
                     /translation="MTTSPAKPKGAPEGAAVLFFVQTFATLGYAVLQSTLVLYATKHL
                     GFSDQLATALMGVFGAFNYGLHLFGGYLGGRFLSNRNLFVGGMALQVFGCACIAMGSA
                     SMMYWGLALFLTGSGLNVTCINMMLTQRFAPDDERRESAFLWNYAGMNIGFFIGFTVA
                     GYFQKDQEYSKLFTFATVGNFTAIVLAAWFWKTLADRSTPLLDKPPAEWRRRFLAGLA
                     ILVGLVPIVWVLLQHPGITETLVKAIGAAVLALLIWLTMRHPVRGERNNMWAYLILAL
                     GSLVFWSLYQLQPSGLQLFFDRNVDPMVFGVKISPQWVQNINTFVIAIGGPIMAWLFT
                     ALRERGWTIDVPKQFAAAVVLMGLGFLALPAGIALADPATGRSAFFWIFLSYLLQSVG
                     ELLISPVGYAMIGKLAPRQYQGIMMGAWMLVTGIASLFAGDFSSDVPPDSGPKATNAF
                     YAALFGKLGWWTVGAGVLLALLIPFLRRLIRGKDTPEVDELAPAVVPH"
BASE COUNT          309 a          614 c          674 g          239 t
ORIGIN      
        1 gccgcggcgc acgaggcgct gcgtgtcgag caggcgcacg cgaacgacgg tgcggcgcgc
       61 cattgcagcg cctgcggcgc gaaggcgcag cccggagcgc gcttctgcag cgcctgcggg
      121 acggcgctgc cggccgcctg atcttcccgg ggacttgcgc gggaatcccg cgcgcctcgt
      181 gccgcgagcg gccgcgcccg gttcgtccag cgggcctcag tgcggcacga ctgccggggc
      241 cagctcgtcg acctccggcg tgtccttccc gcggatcagc cggcgcagga acgggatcag
      301 cagcgccagc aacacgccgg cgccgacggt ccaccagccg agcttgccga acagcgccgc
      361 gtagaacgcg ttggtcgcct tcgggcccga gtccggcggc acgtccgagg agaagtcgcc
      421 tgcgaacagc gaggcgatgc cggtgaccag catccaggcg cccatcatga tgccctggta
      481 ctggcggggc gcgagcttgc cgatcatcgc gtagccgacc ggggagatca gcagctcgcc
      541 gacgctctgc agcaggtagc tcaggaagat ccagaagaac gccgagcggc cggtggccgg
      601 gtcggcgagc gcgatgccgg ccggcagcgc gaggaagccc aggcccatca gcaccaccgc
      661 ggcggcgaac tgcttgggca cgtcgatcgt ccagccgcgc tcgcgcagcg ccgtgaacag
      721 ccaggccatg atcgggccgc cgatcgcgat gacgaaggtg ttgatgttct gcacccactg
      781 cggcgagatc ttcacgccga acaccatcgg gtcgacgttg cggtcgaaga acagctgcag
      841 gccgctcggc tgcagctggt acagcgacca gaacacgagc gagcccagcg cgaggatgag
      901 gtaggcccac atgttgttgc gctcgccgcg cacggggtgg cgcatcgtga gccagatcag
      961 cagcgcgagc accgcggcgc cgatcgcctt gaccagcgtc tcggtgatgc ccgggtgctg
     1021 cagcagcacc cagacgatcg gcaccaggcc cacgaggatg gcgaggccgg cgaggaagcg
     1081 gcggcgccac tccgcgggcg gcttgtccag cagcggcgtg ctgcggtcgg ccagcgtttt
     1141 ccagaaccag gccgcgagca cgatcgcggt gaagttgccg accgtcgcga aggtgaacag
     1201 cttcgagtac tcctgatcct tctggaagta gccggcgacc gtgaagccga tgaagaagcc
     1261 gatgttcatg cccgcgtagt tccagaggaa cgcgctctcg cggcgctcgt cgtcgggcgc
     1321 gaagcgctgc gtgagcatca tgttgatgca cgtcacgttc aggccgctgc cggtcaggaa
     1381 cagcgcgagg ccccagtaca tcatcgacgc cgagcccatc gcgatgcagg cacagccgaa
     1441 cacctggagc gccatgccgc cgacgaacag gttgcggttg ctcaggaagc gcccgccgag
     1501 gtagccgccg aacaggtgca ggccgtagtt gaacgcgccg aacacgccca tcagcgcggt
     1561 ggcgagctgg tcgctgaagc ccaggtgctt ggtcgcgtac agcacgagcg tcgactgcag
     1621 cacggcgtag ccgagcgtcg cgaaggtctg gacgaagaac aggacggcgg cgccctcggg
     1681 tgcgcctttc ggcttcgcgg gggaggtggt catgcaggcg ctttcgggga tggatcgggc
     1741 gcgacggccg gcacgcggga tcgccgcgcg ccgccgagcc gggaggctgg tagcgggtgc
     1801 gcgatgatag cgccgccggg caggccgtcc tggccg
//
LOCUS       DASVLN010000834        11914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_365593, whole genome shotgun sequence.
ACCESSION   DASVLN010000834 DASVLN010000000
VERSION     DASVLN010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11914
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365593"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..460
                     /locus_tag="VF453_19645"
     CDS             137..460
                     /locus_tag="VF453_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7689931.1"
                     /translation="MKEDEVIQSLAALAHPLRLRLFRALVVVGPEGMTPGTMTAALDV
                     SAPALSFHLKELAHAGLVTQERLGRNLVYRAAFDRMSGLLGYLTENCCQGQACLAAAV
                     PGCAC"
     gene            477..956
                     /locus_tag="VF453_19650"
     CDS             477..956
                     /locus_tag="VF453_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEX7689932.1"
                     /translation="MKRFHVHAHVADLAASISFYSRLFGAEPARVEADYAKWMLEDPR
                     LNFAISTRGGAPGVDHLGFQVDEADELPALKERALAAGQAVLDEGETTCCYARSEKHW
                     VTDPQGIAWEHFHTLGDVPVFGQSKPDTAPPVAGGCCGSGDKPRAVGIPVKPASSCC"
     gene            975..1481
                     /locus_tag="VF453_19655"
     CDS             975..1481
                     /locus_tag="VF453_19655"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX7689933.1"
                     /translation="MTTHVLILCTHNSARSVLAEAMLAHWAARLGRDVRAHSAGSAPS
                     GRVNPLALEVLREAGIETSALRSKSWDEFAAEGAPPLAIVITVCDGAAAEACPVFLGG
                     VGGAPVRVHWRYPDPSNAAGGDATRRQAFELTRQALGYRMLQLLQLPLETLAGDELRQ
                     ALAAIARS"
     gene            1484..2203
                     /locus_tag="VF453_19660"
     CDS             1484..2203
                     /locus_tag="VF453_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEX7689934.1"
                     /translation="MALSRRLAAEALGTSLLLAVVIGSGVMGERLAGGNAAVALLANT
                     GATVAGLFVLIEVFGPLSGAHFNPAVSAAMAWRGDLPRAWLLPYVGAQLLGAVLGAWL
                     AHAMFGLPVLQLSGKLRDGSGQWLAEVVATAGLLLVILRAPTTRVSALVACFIGAAYW
                     FTASTSFANPAAVLGRMCSDTFAGIAPRSAPAFALAELAGAAIGVLLDAVLQTPSSSR
                     SRPNPVEASGIAGPDPSTTEP"
     gene            2200..2754
                     /locus_tag="VF453_19665"
     CDS             2200..2754
                     /locus_tag="VF453_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HEX7689935.1"
                     /translation="MTLRIAHPDDAEAIAAIYGPVVAHTAISFELESPSADEMRARIV
                     ATLRHLPWFVSLDDRGLVDGYAYAGRHRERAAYQWSVDTTAYVRADSRGRGVGRRLYA
                     ALLAELLDLGYREAFAGIALPNDASVGLHEAMGFERIGVYRRVGYKLGAWRDVGWWQK
                     PLGEPVEDPAPPRAVGADRKLSHF"
     gene            2844..3701
                     /gene="istA"
                     /locus_tag="VF453_19670"
                     /pseudo
     CDS             2844..3701
                     /gene="istA"
                     /locus_tag="VF453_19670"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            4274..5080
                     /gene="istB"
                     /locus_tag="VF453_19675"
     CDS             4274..5080
                     /gene="istB"
                     /locus_tag="VF453_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX7689936.1"
                     /translation="MRDVMLELKQLRLHGMAGAWADLNEQSGDNGLDTSRWLVEHLLQ
                     AESVDRGMRSVKHQMHVAKFPMHRDLAGFDFDVSPVDRKLVMKLAEMELTLQAHNVVF
                     VGGPGTGKTHLATALGVAGITKHGRRVRFHSTVDLVNALERGKAQGRAGRIADSLLRM
                     DLIILDELGYLPFSQSGGAMLFHLLSRLYEHTSVLITTNLDFAEWSTVFGDAKMTTAL
                     LDRLTHHCHIVETGNESHRFLHSTSVAKKKIRAREHARKGPDTPASDANG"
     gene            5247..5387
                     /locus_tag="VF453_19680"
                     /pseudo
     CDS             5247..5387
                     /locus_tag="VF453_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019437692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6132..6599
                     /locus_tag="VF453_19685"
     CDS             6132..6599
                     /locus_tag="VF453_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7689937.1"
                     /translation="MKLDALDVKILNLLQANAELQMSEIAEQVGLSSTPCWRRIQRMK
                     DAGVITKQVCLLDAKKVNVGVTVFVSVRTSVHTDDWFRQFHATVQAIPEVVEFYRMSG
                     DVDYLLRVVVPDIAAYDSVYKRLIAGTNLADVSSSFAMEELKLTTALPLSYAS"
     gene            complement(6628..7629)
                     /locus_tag="VF453_19690"
     CDS             complement(6628..7629)
                     /locus_tag="VF453_19690"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX7689938.1"
                     /translation="MNYEAIETAAAAWLARQDNTGTWTKDDQRALSEWLAQNTAHRVA
                     WLRLRQAWVRADAMADMPNTHETEEIEIPRLPLSVAPPKRSRRLAYFAGVTMMTIAAV
                     AAYLGTAGRLATQGVEQYATAVGARRQLNLADGSHVLLNTRTRARAEVSRSDRQFWLD
                     DGEAFFEIAHDPAHPFVIRAGNDRITVLGTKFSVRHENGRTQVTVLEGKVRLDHLGTQ
                     VAKPAPEETTTLVKNDTAVSQKGDVVVATRTPQQTLQDLSWRSGRIQFDNRPLVDIAA
                     EFNRYNRRQIVVHEDAAQLRLSVDFDSSNVDGFTRLIHTGFGVVVHANGDQIDLSMS"
     gene            complement(7653..8261)
                     /locus_tag="VF453_19695"
     CDS             complement(7653..8261)
                     /locus_tag="VF453_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7689939.1"
                     /translation="MDGLQLIVGGRLSADVIDDWFSNQVLPLEPALMRLLRRNWRQAD
                     DHADLRQEIYVRVYESALRDGVPESTAPFVFACARNLLIDRVRRAQVVPFEFIADLDD
                     LPEQPDAGFTPEQLADARQEFRLLQIALDELPPRCREIIVLRKIDGLSHKEIAAKLGI
                     AEGTIEKQVTLGVRALAETLYAQGIEAAAAWVRRVRRGACEQ"
     gene            complement(8437..9291)
                     /locus_tag="VF453_19700"
     CDS             complement(8437..9291)
                     /locus_tag="VF453_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689940.1"
                     /translation="MSGAFHNDAGLKAQACSEAASGANPGWSVVATDEERAACSATYD
                     LHASFINLLSHGFHVPYGEDALPLLERALLSLAPGADTLALVRNWVIRLWVDPEIGMK
                     ARLDGTVAFTPAEQVIRLVRRSAQEPVARDEWRRARGALNSVKAQAPEVAPYVELVAA
                     MAWDLASTPKVTTDIWNAWQASVGSVLDRKAGWDKDKQDEVLEAFRRSLHEAHEEVGQ
                     VSAEVAASAEHNARINALVQAKLEAAGQAGNFQSLQSHFASTMTPAMMRWRETAHQSL
                     LDACRMAA"
     gene            complement(9297..9626)
                     /locus_tag="VF453_19705"
     CDS             complement(9297..9626)
                     /locus_tag="VF453_19705"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7689941.1"
                     /translation="MDEFSVVPESDFAKSVLDSTAPVVVDFFSKTCGPCKLLEAALKD
                     VAPDYDGEIKVVKVDVEESPAVARQYSIQSVPTMLLFRKGRLTQRVLGNQTRSKLAEL
                     FDAHVAE"
     gene            complement(9817..11763)
                     /locus_tag="VF453_19710"
     CDS             complement(9817..11763)
                     /locus_tag="VF453_19710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7689942.1"
                     /translation="MRLSDTALVPRAKLFGNPERGGCQVSPCGRWLAFIAPVNGVMNL
                     WVCELGSDLQASLSAARPITDDRGRGIYVFSWAFDGVHLIYLQDKAGDEDTHVFAVPA
                     AGGTARDLTPFERTRAIVQGLSRKHPGTVLVSMNRRDARYGDVYRLELASGELELVAE
                     NPGFFGFMTDDDFNVCLAVAPNEDGGKLLLTREGDAWKPWRHIAAADTSNTGPQGLNV
                     DGHTLYMFDSRGRDTAALMSYDLRGSSEEGTLLAEHRGADVIDVWADLETHAPLAWTA
                     TIERREVHVLDDRIREDVAFLNARGLGEWGLASRTTDDRQWIVVASNDVTPSTFYFFD
                     RPSKNLVKLYDARPELRGAPLARMQHTTISSRDGLRMVTYLTVPIHADRLDRPLSSNV
                     PLPLVLLVHGGPQMRDWWGYNPMHQWLANRGYAVLSVNFRASTGFGKAFVTAGDGQWG
                     AKMDDDLVDAVRWAIDRGIADPKRICIMGGSYGGYATLWGMTANPELYACGVDIVGPS
                     NLETLAASIPPYWAAAKDQFYRMIGNGETPEGRALMRERSPVHRAGLIQRPLLIGQGA
                     NDPRVKQAESDQMVAAMKANGVPVTYVLFPDEGHGFHRPANNIWFNALTERFLEKYLG
                     GRSEPLSDGEVEGNTAVLVEDSIG"
BASE COUNT         2075 a         3952 c         3851 g         2036 t
ORIGIN      
        1 ggcggtcggg caaggtcgaa acgcggagcg cgacgaggtc ctgcaggcgc tctcgcggca
       61 gtgacgcgat gcgccggaga ggcgaccgct tcggccggcg cttgcatcga ttcaataatt
      121 caagtatcat ttaaacatga aggaagacga agtcatccag tcgctggccg cgctggcgca
      181 tcccctgcgc ctgcgcctgt tccgcgccct ggtcgtcgtc gggcccgaag gcatgacccc
      241 gggcacgatg acggcggccc tcgacgtgtc cgcgccggcg ttgtccttcc acctgaagga
      301 gctggcacat gccggcctcg tcacccagga gcggcttggc cgcaacctgg tctaccgcgc
      361 cgcgttcgac cgcatgagcg gcctgctcgg ctatctcacc gagaactgct gccaggggca
      421 ggcgtgcctt gcggccgccg tcccgggctg cgcctgttga accgaaggag ccctccatga
      481 agcgattcca cgtccacgcc catgtcgccg acctcgcggc cagcatctcg ttctactcgc
      541 ggctcttcgg cgcggaaccc gcgcgcgtcg aggccgacta cgccaagtgg atgctcgagg
      601 atccgcgcct gaacttcgcc atctcgacgc ggggcggcgc accgggggtc gatcacctcg
      661 gcttccaggt cgacgaggcc gacgagctgc ccgcgctcaa ggaacgcgcg ctggccgccg
      721 gccaggcggt cctcgacgaa ggcgagacga cctgctgcta cgcccgcagc gagaagcact
      781 gggtcaccga cccgcaaggc atcgcctggg agcacttcca cacgctgggc gacgtcccgg
      841 tcttcggcca gagcaagccg gacacggcgc cgcccgtcgc gggcggctgc tgcggatcgg
      901 gcgacaagcc ccgggccgtg ggcatcccgg tcaagcccgc ttcgagctgc tgctgagccg
      961 tccgggcccg cgccatgacc acgcacgtcc tcatcctgtg cacccacaac tcggcgcgca
     1021 gcgtcctcgc cgaggccatg ctggcccact gggcggccag gctcggccgc gacgtgcgcg
     1081 cccacagcgc gggcagcgca ccgagcggcc gcgtcaaccc gctggcgctg gaggtgctgc
     1141 gcgaggcggg catcgagacg tcggccttgc gcagcaagtc ctgggacgaa ttcgccgccg
     1201 agggcgcgcc gccgctggcg atcgtcatca cggtctgcga cggcgcggcc gccgaggcct
     1261 gcccggtctt cctcggcggc gtgggcggcg ccccggtcag ggtgcactgg cgctatcccg
     1321 atccgtcgaa cgccgccggc ggcgacgcga cgaggcgcca ggcgttcgag ctcacgcgcc
     1381 aggcgctcgg ctaccggatg ctgcagctgc tgcagctgcc gctcgagacc ctcgcggggg
     1441 acgagctgcg gcaggcgctc gccgcgatcg cgcggagctg acgatggcgt tgtcgcgcag
     1501 gctggcggcg gaggcgctcg gcacctcgct gctgctggcc gtcgtcatcg gctcgggcgt
     1561 gatgggcgag cgcctggccg gcggcaacgc cgcggtggcc ttgctggcca ataccggggc
     1621 gaccgtggcg gggctgttcg ttctcatcga ggtgttcggg cccctcagcg gcgcgcactt
     1681 caacccggcg gtcagcgcgg cgatggcctg gcgcggcgac ctgccccggg catggctgct
     1741 cccctacgtc ggcgcgcagc tgctcggtgc ggtgctgggc gcctggctcg cgcacgccat
     1801 gttcggcctg cccgtgctgc agctgtccgg aaagctgcgc gacggcagcg gccagtggct
     1861 cgcggaagtc gtggcgacgg cgggcctgct gctcgtgatc ctgcgcgcgc cgacgacgcg
     1921 ggtctcggct ctcgtggcgt gcttcatcgg cgccgcctac tggttcaccg cctcgacgtc
     1981 gttcgcgaat ccggccgccg tgctcgggcg gatgtgcagc gacaccttcg ccggcatcgc
     2041 gccgcgcagc gcgccggcct tcgcgctggc cgaactggca ggcgccgcga tcggcgtcct
     2101 gctggatgcg gtgctccaga cgccgtcgtc gtcccgctcg cgtccgaacc ccgtcgaggc
     2161 ctccggcatc gccggccccg atccttcgac gaccgagcca tgaccctgcg catcgcccat
     2221 cccgacgacg ccgaggccat cgcggccatc tacggcccgg tggtcgcgca caccgccatc
     2281 tcgttcgagc tcgagtcgcc gtccgccgac gagatgcgcg cgcggatcgt cgccacgctg
     2341 cggcacctgc cgtggttcgt gagcctcgac gatcgcggtc tcgtcgacgg ctacgcctac
     2401 gcgggccggc atcgcgagcg cgccgcctac cagtggtcgg tcgacacgac cgcctacgtg
     2461 cgcgccgaca gccgcggccg gggcgtcgga cgcaggctct atgcggcgct cctggcggaa
     2521 ctgctcgacc tgggataccg cgaggccttc gccggcatcg cgttgccgaa cgacgccagc
     2581 gtcggactgc acgaggcgat gggcttcgag cgcatcggcg tgtaccgccg cgtcggctac
     2641 aagctcggcg cgtggcgcga cgtcggatgg tggcagaagc cgctcggcga gccggtcgag
     2701 gatcccgcgc cgccccgggc tgttggcgcc gaccgaaaat tgagccactt ttgaggggtg
     2761 tgccgaccga aaactgagcc aggtgtttga cctaacctgc tgcttttttg tgcagcgggg
     2821 ttcgaggatt gatcaccatg gacatgatcg gaaggatccg gcggctgcac agccggggca
     2881 acaagagcga gcgcgagatt gccaggctga cgggtctgtc gcgcaacacg gtggccaagt
     2941 ggctgaaggc gccggcggag acggctccga agtaccagcg caaggcagcc gtgaccaagc
     3001 tgacgccgtt ccacgaggcg ctcaagcagg cgctgcgcgc cgatgcgcgg cgaccggtcg
     3061 cggcccggcg cacggccaag gcgctgtacg ccgaggtcaa ggcggccggc tacgccggtt
     3121 gctacacacg ggtgaaggat ttcatccggg agtggcgcgc cggcgaaggc cagtcggtgg
     3181 tcgcgcgcgc attcgtgccg ctgacgttcg cgctgggcga ggcattccag ttcgactgga
     3241 gcgaggaagg tcaagtggtg ggcggcgtct accaccgcat gcaggtggcg cacatgaagt
     3301 tgtgcgcctc gcgcgcgttc ttcctggtgg cctacccgag ccagggccac gagatgctgt
     3361 tcgacgccca tacgcgcagc ttcgccgcgc tgggaggggt gccgctgcgc ggcatcttcg
     3421 acaacatgaa gacggccgtc gacaaggtca acaagggcaa ggaacgcgtg gtcaacgagc
     3481 gcttcgcggc gatgtgcgcg cactacttgt tcgacccgga cttctgcaac gtggcctccg
     3541 gctgggagaa aggcgtcgtc gagaagaacg tgcaggacag ccggcgacgc atctggatcg
     3601 aggccggcaa gcagcgcttc ggctcgttcg tcgagctcaa cgcgtggctg gcagcgcgct
     3661 gccgggcgct gtgggaggaa ctgcgccatc ccgagcacga gcagttcacc atcgccgaga
     3721 tgctcgagca cgagcgagcc gcgctgatgc cgatgcccga accgttcgac ggctacgtcg
     3781 agcggccggc acgggtgtcg agcacgtgcc tggtgaccgt ggcgcgcaac cgctactcgg
     3841 tgccgtgcga gttggccgga cagatggtca gcgcgcggct gtaccctgac gaggtggtga
     3901 tcgtggcagg cgacgcgacc gtttgcgcag cgcgccgggc ttgcgctgca ccagcgggac
     3961 gtagtggcgc cagtcgtacg cggtctgctc gcgtgcctgc agtcggcggt gccgcgcaac
     4021 cgggtgatgg cgcaggtgct ggccctcgtg cccagcgccg gcctggaggc cgtgctgatc
     4081 gccgtggatc tggcgctcga gagcgcgccg ccgtccgggc gcgtcagcgc cgagcacgtc
     4141 ctcaacgtgc tgggccggtt aaacgccgcg gcgccgccgg agaacgcggt caccgcgctg
     4201 aaagtgacga caccgccgct ggccgatacg gcgcgctacg acagcctgcg gattcaggcc
     4261 gaggaggccg gccatgcgtg acgtgatgct ggagctcaag caactgcgcc tgcacggcat
     4321 ggccggcgcg tgggccgacc tgaacgagca gagcggcgac aacggtctgg acacctcgcg
     4381 ctggctggtc gaacacctgc ttcaggccga gagcgtggat cgcggcatgc gctcggtcaa
     4441 gcaccagatg catgtggcga aattcccgat gcaccgcgac ctcgcgggct tcgacttcga
     4501 cgtctcgccc gtcgaccgca agctggtcat gaagctggcc gagatggagt taaccctcca
     4561 ggcgcacaac gtcgtcttcg tcggcggacc gggcaccggt aagacgcacc tggccacggc
     4621 actcggcgtg gccggcatca ccaagcacgg ccggcgcgtg cgcttccact cgacggtcga
     4681 cctggtcaac gcgcttgagc gcgggaaggc ccagggccgc gccgggcgca tcgccgacag
     4741 cctgctgcgc atggatctca tcatcctcga cgagctcggc tacctgccgt tcagccagtc
     4801 cggcggcgcg atgctgttcc acttgctcag ccgactttat gagcacacaa gcgtgctgat
     4861 caccacgaac ctggacttcg ccgagtggag cacggtgttc ggcgacgcca agatgacgac
     4921 cgcgctgctg gaccggctca cgcaccactg ccacatcgtg gagaccggca acgagagcca
     4981 ccgcttcctg cacagcacct cggtggccaa gaagaagata cgagcacgtg agcatgctcg
     5041 caaagggccc gacacgccgg cgtccgacgc caacgggtga ctgagccggc gcggggcgag
     5101 ccgcgccgtt ctggagacgt tatgaggagg caggcaacga agacaaagac gtagacttat
     5161 ccacagccga ccgctgcgcg gacggcttcc ggacctggct caaatttgag tcggcacggt
     5221 ggctcaaaat tcagtcggcg ccgacagtgg agcacccctt aagggttctc aaacggcagt
     5281 tcggatatac gaaggtgcgc taccgaggac tggccaagaa cacggcccag atcatcacgc
     5341 tcttcgcgct gtcgaacctc tggatggcgc gccgaaagct cgtttgaggg ctgctgaagc
     5401 ggcctcgcga ggccacatcg gggggctcac ggcgcctccg ccagctcatg cagcccgctc
     5461 tgaggagcgt caaagccttg gagcggcggc ccggacgtca aatagtccgt caacagcgtt
     5521 gtgcagacct tcccttacgg gaatctggtc agcggttcgt cctcaatgat gaagcgtgca
     5581 atccacttct acgatgttca ccagtaaatc accagcttaa tgccacttga gaaccacttg
     5641 tgggccgact tcccgggcac gtcgttcgac gccgagaggg gttgaatcaa gcccattgac
     5701 agcccattgc gctttggcac agaacgagct cgaaacacgc aatctggttc tccctttagc
     5761 acggaaaaat cgtctctcga agtgctccaa agtcaggacg cgatcgggtc gcaacccggc
     5821 cagaggacag ccgttgattc aaaaacgtac gccgctttcg cgtcgacctg gctgccaacc
     5881 tgcagcaacc ttcgtggctt cagagaactg ctggatcgac agtgaactga gggtgcgttg
     5941 agaagctgca attgtcgttg ttctgtggag gccgaccacg agtcggaagg cgctccagcg
     6001 tagaacagcg gataggttaa agatgcctat ccgccgctct cgcggcgttc ccgcagccgc
     6061 agctcaagtc ctttgcaacc gccacgcgtg tgccacatat ccgtcctagc gaaaactcaa
     6121 gaccaggaac gatgaagctc gacgcactgg acgtgaagat tctgaatctg ctgcaggcga
     6181 acgccgagtt gcagatgagt gaaatcgccg agcaagtcgg actgtcgtcc actccgtgct
     6241 ggcgccgtat ccagcgaatg aaggatgccg gcgtcatcac gaagcaggtc tgcctgctgg
     6301 acgccaaaaa ggtgaacgtg ggagtgacgg tcttcgtttc ggtacgtaca agcgtccata
     6361 ctgacgactg gttcaggcag ttccatgcga cggtgcaggc gattcccgag gtggtcgagt
     6421 tctaccgcat gagtggcgac gtcgactatc tcctgcgcgt ggtggtgccc gacatcgcgg
     6481 cctacgactc ggtatacaag cgcttgatcg ccggcacgaa cctcgccgac gtcagctcca
     6541 gcttcgcgat ggaggagctc aagctcacga cggcgctacc actcagctat gcatcctgac
     6601 gcggcggcct gctcggccga attcggctca gctcatcgac agatcgatct gatcgccgtt
     6661 ggcatgaacc accacaccga atccggtgtg aatgaggcgc gtgaatccgt cgacattgct
     6721 cgaatcgaag tcgacgctca gtcgcaactg cgccgcatct tcgtggacga cgatctgacg
     6781 ccggttgtac ctgttgaatt cggccgcgat atcgaccaac ggcctgttgt cgaactggat
     6841 ccggccggac cgccagctca agtcctggag cgtctgctgc ggcgtccggg ttgccacgac
     6901 gacgtcaccc ttctgggaaa ccgcagtgtc gttcttgacc agggtggtcg tctcctcagg
     6961 ggcgggcttg gccacctggg tacccaggtg gtcgagcctg accttgccct cgagcacggt
     7021 gacttgcgtg cgcccgtttt cgtgccgcac gctgaacttg gttccgagaa cggtgatgcg
     7081 atcgtttccc gccctgatga caaaggggtg cgccggatcg tgcgcgatct cgaaaaacgc
     7141 ctcgccatcg tcgagccaga actgccgatc gctccgactg acctccgcac gcgcacgtgt
     7201 tctggtgttc agaagaacgt gcgatccgtc agccaagttc agctgtcggc gggcgcccac
     7261 tgccgtcgcg tattgctcga caccctgcgt cgcaagcctg cccgcggtcc ccaggtaggc
     7321 ggccaccgcg gcgatcgtca tcatggtgac gccagcaaag taagccagtc tccgagaccg
     7381 cttcggcggt gccaccgaca ggggcagacg cggaatttcg atctcttccg tctcgtgggt
     7441 gtttggcata tcggccatgg catcggcacg gacccacgcc tggcgcagtc gtagccaggc
     7501 cacacgatga gccgtgttct gcgcgagcca ctccgagagg gcgcgctggt cgtcctttgt
     7561 ccacgtcccc gtgttgtcct gccgagccaa ccaggcggcc gctgccgtct cgatggcttc
     7621 gtagttcacg gagcttgctg atacgttcct cttcattgct cgcatgctcc acggcgaaca
     7681 cggcgaaccc aggccgcggc cgcttcaatc ccttgcgcgt acagcgtctc cgccagcgcg
     7741 cgcactccca gtgtcacctg cttttcgatc gttccttcgg caatcccgag ctttgcagcg
     7801 atctccttgt gcgagagacc gtcgatcttt cggaggacga tgatctcccg acagcgcgga
     7861 ggaagctcgt cgagcgcgat ttgcagaagc cgaaactctt ggcgggcgtc agccagttgt
     7921 tccggtgtga agcccgcatc gggttgctcg gggaggtcgt cgaggtctgc gatgaactcg
     7981 aagggcacca cctgcgctcg acgcacccga tcgatgagga ggttgcgcgc gcaggcgaag
     8041 acgaatgggg ccgtgctctc aggaacaccg tcgcgcaagg ccgactcgta cacgcgaacg
     8101 tagatctcct gcctcagatc cgcgtgatca tctgcctggc gccagttgcg ccgcagcaaa
     8161 cgcatcagtg ccggctcgag aggcaggacc tggttggaga accaatcgtc gatcacgtcc
     8221 gcagaaaggc ggccgcccac aatgagctgc aggccgtcca attcggagtc atttgcggat
     8281 ttcaccctag tccagacgaa cggcctacgg agatcccgta aggcgaaggc cacttttctc
     8341 gaaaaagtgg cctcgggggg gctatcccgc ctctttcacg cggggtacga agcggcagtc
     8401 gcacaaggta ggtataaacc cttaccccag tcagcgtcac gcggccatgc ggcaggcatc
     8461 gagcagggac tggtgcgcgg tctctcgcca cctcatcatt gccggcgtca tggtggatgc
     8521 gaagtggctc tgcaagcttt ggaaatttcc cgcttgcccg gccgcctcca acttggcctg
     8581 tacgagtgcg ttgatccgtg cgttgtgttc cgcggaagct gcaacctccg cagagacctg
     8641 accaacctct tcgtgcgcct catggagcga tcgacgaaaa gcctcgagaa cctcgtcttg
     8701 cttgtccttg tcccagcccg cctttcgatc aagcacggag cccaccgacg cctgccaggc
     8761 gttccatatg tccgtggtca ccttcggtgt ggaggcaaga tcccaggcca tcgcggcgac
     8821 taactcgaca tacggcgcca cctcaggcgc ctgcgccttg accgaattca acgcgcctcg
     8881 tgcgcgacgc cattcgtccc gcgcgaccgg ctcctgtgct gaccggcgca ccagtcgaat
     8941 gacttgctcg gccggcgtga acgcaacggt tccgtccagt cgcgccttca tcccgatctc
     9001 gggatccacc cagaggcgta tgacccagtt gcgaaccaga gcgagcgtgt cggcgcccgg
     9061 cgccagggaa agcagcgcgc gctcaagcag cggcagcgca tcctcgccgt agggcacgtg
     9121 aaagccatgc gacagcagat tgatgaagct tgcgtgcagg tcatacgtgg ccgagcaagc
     9181 cgcccgctct tcgtccgtgg caacgacgga ccagccggga ttggcccccg acgctgcctc
     9241 cgagcacgcc tgggccttca gccctgcgtc attgtgaaac gcaccgctca cctctgctac
     9301 tccgccacgt gcgcgtcgaa cagctcggcg agcttgctgc gggtctggtt gccaaggacg
     9361 cgctgcgtca accgcccctt gcggaacagc agcatcgtcg gcacgctttg gatgctgtac
     9421 tgccgcgcaa cggccggaga ctcctccaca tcgaccttga cgaccttgat ctcgccatcg
     9481 tagtccggcg cgacatcctt cagagccgct tccagcagct tgcaaggccc gcaggtcttc
     9541 gagaagaaat ccaccacgac gggcgcagtc gagtcgagca cgctctttgc gaaatcgctc
     9601 tccggaacca cagaaaactc atccattttc ttctccgcta ggagtgaatc aacaaggaaa
     9661 tgatgcgctg gcgaacggac aaccgctttg cgagtgcgcc tgcacttgcg gcgaatggcg
     9721 caatctggtt gctccgcgtc cggcgccgcg tagatttgca ttgcgcgccg cccgtcctgc
     9781 aaaccgcagc cgcgcggcgc gcttcgctcg ctcttgctac ccgatcgagt cttcgaccag
     9841 aactgccgtg ttgccttcga cctcgccgtc cgacagtggt tcacttcttc caccgagata
     9901 cttctcgaga aagcgttcgg tcagggcgtt gaaccagatg ttgttggccg gacggtggaa
     9961 gccatgcccc tcgtccggga agagtacgta ggtcaccgga acgccgttgg ccttcatcgc
    10021 agcgaccatc tggtcgctct cggcttgctt gacgcgggga tcgttcgcac cttgaccgat
    10081 cagcagagga cgctgaatga ggccggcccg atgaacaggg gagcgctcgc gcatgagcgc
    10141 gcgcccctcg ggcgtctcgc cgttcccgat catccggtag aactggtctt tggcggccgc
    10201 ccagtatggc gggatcgacg cagcaagcgt ctcgaggttg gacgggccga cgatgtcgac
    10261 gccgcacgcg tagagttccg ggtttgccgt cataccccac agcgtggcat agccgccgta
    10321 gctgccaccc atgatgcaga tgcgcttcgg gtctgcaatg cctcggtcga tagcccagcg
    10381 aacggcgtcc accagatcgt cgtccatctt ggcaccccac tgtccgtcac cggccgtgac
    10441 gaaggccttg ccgaagcccg tcgacgcccg gaagttgacg ctgagtacgg cgtatcctcg
    10501 attggccagc cactggtgca tcgggttgta gccccaccaa tcgcgcattt gggggccgcc
    10561 gtgcaccaac agcacaagag gaagcggcac gttgctggac aggggccggt cgaggcgatc
    10621 cgcatggatc ggaactgtca ggtaggtcac catgcgcagg ccatcgcggc tgctgatggt
    10681 ggtgtgctgc atacgcgcca gaggagcgcc gcgcagctca ggtcgtgcgt cgtacagctt
    10741 gacgagattc ttcgacggac gatcgaagaa atagaaggtc gaaggggtga cgtcgttcga
    10801 ggccacgacg atccattgcc ggtcgtctgt cgtccggctc gccagtcccc actcgccaag
    10861 acctctggcg ttgagaaagg ccacatcttc ccgaatccgg tcatcgagga catggacctc
    10921 gcgtcgctcg atcgttgcgg tccaggccag cggcgcgtgc gtttccagat cggcccacac
    10981 gtcgatgaca tcggcaccac gatgctctgc tagcaacgtt ccttcttccg acgatccgcg
    11041 caggtcgtag ctcatcaagg ctgcagtgtc gcgaccgcgg ctgtcgaaca tgtacagcgt
    11101 gtggccatca acattgagcc cctgcggccc agtatttgac gtgtccgccg ccgcaatgtg
    11161 ccgccagggc ttccaggcat cgccctcgcg cgtgaggagc aactttccac catcctcatt
    11221 cggcgcgacg gcaaggcaga cattgaagtc gtcgtccgtc atgaatccga aaaagccagg
    11281 gttctcggcg acgagctcca gttcaccact ggccagttcc agtcggtaga cgtcgccata
    11341 gcgcgcatcg cgccggttca tcgaaaccag gacggttccg ggatgcttgc ggctgagacc
    11401 ctgaacgatc gcgcgagtgc gctcgaatgg ggtgagatcg cgtgctgttc caccagcggc
    11461 agggacggca aagacgtgcg tgtcttcgtc accggccttg tcctgcagat aaatcaggtg
    11521 aacgccgtcg aacgcccagc tgaagacgta gatccctcgc cctcggtcgt cggtaatcgg
    11581 acgagcagcg ctcagcgacg cctgcaagtc gctgcctagc tcgcacaccc aaaggttcat
    11641 cacgccattg accggcgcga taaacgccaa ccagcggccg cacggactaa cttggcagcc
    11701 acctcgttct ggattgccaa agagcttggc gcgaggcacc agcgcagtat cactcaaacg
    11761 catgtcagat gatcctcaga gggtgtagct gaaatcaaca cttcacacgc tacagcttgc
    11821 acaatagctg aagttgcgag gtgactgatg aacgacaagc cgagacaggg cggacgaccg
    11881 ccgacgctca cgccagagca tcgagccgta ctgg
//
LOCUS       DASVLN010000835         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_419493, whole genome shotgun sequence.
ACCESSION   DASVLN010000835 DASVLN010000000
VERSION     DASVLN010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419493"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3276)
                     /locus_tag="VF453_19715"
     CDS             complement(<1..3276)
                     /locus_tag="VF453_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7689943.1"
                     /translation="MFLSDFSIKRPIAAIVIIIALMGLGLIALSKLRVNNIPDVQQPL
                     IAVSIDYPGASPDTVEREIVDRIERTFQGIEGVDQARSHAKAVEGNATFILVFNFHKD
                     LTQASDEIRNAIGTVRYKLPIEMREPVLTRIDPSAQPVMQIALSSSTLSHAELSLLAE
                     DRFADRFRAIPNVSVVNVYGARQRELSVLLHAQKLHEFGVSVTDVVNALRMQNATAPV
                     GKVRGELEDQSIRLVGRFESPVEFDQMPIKRSGDDFVRLGQVATVQDGFADADSHNER
                     DGRLNVGMSVSRTRDGSSVSVAKEVRAVVKQINEEYAGKLKMEITEDGGDEAQDSLNN
                     VVHALVFGAGLTILVVYVFLNSWRSTLITGLSLPTSVITAFIAVWLCGFTLNFMSLLG
                     LSLAIGVLIDDAIVVRENIVRHMQRGEDRRTAALRGTAEIGLAVAATTFSIVSVFAPV
                     AFIPGMTGEWFRPFGLTVVASVIVSLLISFTLDPMLSAYWGDPVDLHDAPRKGLGRVL
                     QRFNDWFDHQADRYGAVIQWAMHHRWTMGWVAFLSFAAALVLQGTLGGSEFLPKSDVG
                     TIMVQVRMPPSSSLEYARLKTEAAVALTRQLPEYKNSSAEINAANATVYVDIHKSTER
                     KRSAQQVADDLRDRVRSLVGAEYTVMDDLNNGGQKSVELRFYGADSRKLLAITSDFMD
                     KLRQVRGAVDVELSQQDPQQELRIDLDRGLANRMGVSINDAAQTLRVAFAGVEAGDWV
                     DPTGETRDVAVRLAPQDRVDASNIERLPIAIAGTDRTVPLEQIATVSMGKAPSVIEHA
                     GGKRMVTVTANAQGRSSGELLADAMKIVKATQFPPGYGIELAGNSRDQQEVFANLGIA
                     LVSGVALMYLVLVIQFGSFTAPIPVMISLPLSLIGVVAALLVTHGTLNLMSIIGVIML
                     MGLVAKNAILLLDAARAEERAGTDREEALMHAGRKRLRPILMTTFALIAGMLPVAIGV
                     GEGGEFYRPMAVTIIGGTITSTLLTLLVIPCFYDSIELMRVHLGEKFHARSARFNPVA
                     AFVMCLGEFVAFLTFVRFVYRLVRRLSGRPAGGLWRKEAPKPASVAGTAAAQAAPQAD
                     GGSA"
BASE COUNT          510 a         1148 c         1182 g          503 t
ORIGIN      
        1 cgcggagccg ccgtcggcct gcggcgcggc ttgcgccgcc gccgtgccgg ccacggacgc
       61 gggcttcggg gcctccttgc gccacagccc gcccgcgggc cgtcccgaca gtcgccgcac
      121 caggcggtag acgaagcgca cgaaggtcag gaaggcgacg aactcgccca ggcacatcac
      181 gaaggccgcc accggattga agcgcgccga gcgggcgtgg aacttctcgc ccaggtgcac
      241 gcgcatgagc tcgatcgagt cgtagaagca cgggatcacg agcagcgtga gcagcgtcga
      301 ggtgatcgtg ccgccgatga tggtcaccgc catcggccgg tagaactcgc cgccctcgcc
      361 cacgccgatg gccaccggca gcatgcccgc gatcagcgcg aacgtcgtca tcaggatggg
      421 gcgcaggcgc ttgcggccgg cgtgcatcag ggcctcctcg cggtcggtgc cggcgcgctc
      481 ctcggcgcgc gcggcgtcga gcagcaggat cgcgttcttc gccaccagcc ccatcagcat
      541 gatcacgccg atgatgctca tgaggttgag cgtgccgtgc gtgaccagca gcgccgcgac
      601 gacgccgatc aggctcagcg gcagcgagat catcaccggg atcggcgccg tgaacgagcc
      661 gaactggatc accagcacga ggtacatcag cgcgacgccc gagacgagcg cgatgcccag
      721 gttggcgaac acctcctgct ggtcgcgcga gttgccggcc agctcgatgc cgtagcccgg
      781 tgggaactgc gtcgccttga cgatcttcat cgcgtcggcc agcaactcgc ccgacgagcg
      841 cccctgcgcg ttggccgtga cggtgaccat gcgcttgccg ccggcgtgct cgatgaccga
      901 gggcgccttg cccatcgaga cggtggcgat ctgctccagc ggcaccgtgc ggtcggtgcc
      961 cgcgatggcg atcggcaggc gctcgatgtt cgaggcgtcg acgcggtcct gcggcgcgag
     1021 gcgcacggcg acgtcgcgcg tctcgccggt cgggtcgacc cagtcgccgg cctcgacccc
     1081 cgcgaaggcc acgcgcagcg tctgcgccgc gtcgttgatc gagacgccca tgcggttggc
     1141 caggccccga tcgaggtcga tgcgcagctc ctgctgcgga tcctgctgcg acagctcgac
     1201 gtccaccgcg ccccggacct ggcgcagctt gtccatgaag tccgaggtga tcgcgagcag
     1261 cttgcgcgag tccgcgccgt agaagcgcag ctccaccgac ttctggcccc cgttgttcag
     1321 gtcgtccatc accgtgtact cggcgcccac caggctgcgc acgcggtcgc gcaggtcgtc
     1381 ggcgacctgc tgcgcgctgc gcttgcgctc ggtgctcttg tggatgtcga cgtagacggt
     1441 ggcgttcgcg gcgttgatct cggcgctcga gttcttgtac tcgggcagct ggcgcgtgag
     1501 ggcgaccgcg gcctcggtct tcaggcgggc gtactccagg ctcgaactgg gcggcatgcg
     1561 cacctggacc atgatggtgc ccacgtccga cttgggcagg aactccgagc cgccgagcgt
     1621 gccctgcagc accagcgcgg ccgcgaagct gaggaaggcg acccagccca tcgtccagcg
     1681 atggtgcatc gcccactgga tgaccgcgcc gtagcgatcg gcctggtggt cgaaccagtc
     1741 gttgaagcgc tgcagcacgc gacccaggcc cttgcgcggc gcgtcgtgca ggtcgaccgg
     1801 gtcgccccag taggccgaca gcatggggtc gagcgtgaac gagatcagca ggctcacgat
     1861 cacggaggcc acgacggtca ggccgaacgg gcggaaccac tcgcccgtca tgccggggat
     1921 gaaggccacc ggcgcgaaca ccgagacgat ggagaacgtc gtcgccgcga cggccaggcc
     1981 gatctccgcg gtgccgcgca gcgccgcggt gcggcggtcc tcgccgcgct gcatgtggcg
     2041 gacgatgttc tcgcgcacga cgatcgcgtc gtcgatcagc acgccgatcg ccagcgacag
     2101 cccgagcagg ctcatgaagt tcagcgtgaa gccgcacagc cataccgcga tgaacgccgt
     2161 gatgaccgag gtcggcagcg acaggcccgt gatcagggtc gagcgccacg agttcaggaa
     2221 gacgtagacc acgaggatcg tcagcccggc gccgaagacg agcgcgtgca cgacgttgtt
     2281 caggctgtcc tgcgcctcgt cgccgccgtc ctcggtgatc tccatcttca gcttgccggc
     2341 gtactcctcg ttgatctgct tgacgaccgc gcgcacctcc ttggccacgc tcaccgagct
     2401 gccgtcgcgg gtgcgcgaga ccgacatgcc gacgttcagc cggccgtcgc gctcgttgtg
     2461 cgagtccgcg tcggcgaagc cgtcctgcac cgtcgcgacc tggcccaggc gcacgaagtc
     2521 gtcgcccgag cgcttgatgg gcatctggtc gaactccacc ggcgactcga agcggccgac
     2581 gaggcggatg ctctggtcct cgagctcgcc gcgcaccttg cccaccggcg ccgtggcgtt
     2641 ctgcatgcgc agcgcgttga cgacgtcggt cacggagacg ccgaactcgt gcagcttctg
     2701 cgcgtgcagc agcaccgaca gctcgcgctg gcgcgcgccg tagacgttga ccaccgacac
     2761 gttcgggatc gcgcggaagc ggtcggcgaa cctgtcctcg gccagcagcg agagctcggc
     2821 gtggctcaag gtgctggacg acagcgcgat ctgcatcacc ggctgcgccg acggatcgat
     2881 gcgcgtgagc accggctcgc gcatctcgat cggcagcttg tagcgcacgg tgccgatcgc
     2941 gttgcggatc tcgtcggagg cctgggtcag gtccttgtgg aagttgaaga cgaggatgaa
     3001 ggtggcgttg ccctcgaccg ccttggcgtg cgagcgcgcc tggtcgacgc cctcgatgcc
     3061 ctggaaggtg cgctcgatgc ggtcgacgat ctcgcgctcg acggtgtcgg gcgaggcgcc
     3121 gggatagtcg atgctgaccg cgatcagcgg ctgctgcacg tcggggatgt tgttgacgcg
     3181 cagcttggac agcgcgatca ggcccaggcc catcagcgcg atgatgatga cgatggccgc
     3241 gatcggccgc ttgatgctga agtcggagag gaacatgggg cggctcctgg cgcgtcaggc
     3301 gcgcgcgccc gcggcggtcg cggcggcggt cgaggacgcg gcc
//
LOCUS       DASVLN010000836         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_154825, whole genome shotgun sequence.
ACCESSION   DASVLN010000836 DASVLN010000000
VERSION     DASVLN010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154825"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..>2089)
                     /locus_tag="VF453_19720"
     CDS             complement(102..>2089)
                     /locus_tag="VF453_19720"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7689944.1"
                     /translation="APPPIDPIPPLSAAARLAPPAAPPPALQPGMRNFAGPLVNGTAV
                     LVLLYFGREVLVPVTLAAILSLLLAPLVRRFRRLGLGQVPSVLGAVSVTTLCVLGLAG
                     VIATQVVAMAASLPQYETTIRAKVEVVRELTLGRMEAMRGEAGRMMSRLDGQPPAASD
                     AAPGTIDLARAPLTANGAVPVEIHERPPTPVEVMSRVFSSVWGPLGKAGIVLVILVFV
                     LLEHESLRDRFIRLIGGSDLRGTTHAFNDAGERLSRFFVSQFAVNVGVGAVVWLGAMA
                     IGLPHGMLWAVLTAVLRFVPYVGVFVAAFSAALLAAAVDPGWSMMFLTLGLFAAVELI
                     ASQVVEPQLYGHTTGLSPLSVVLAAIFWSWIWGPIGLLVSTPLTLCLVVLGRYVKSLA
                     FLDILLGDTPALTMSERFYQRALSGDADEVVAAARNYLKRKSLARYCDRILMPALRLA
                     VADFLNGSISGEQQMRVKGTIAQVIEALTAQGCTRGRQRISVLDEPSLGAHLRHRRES
                     ALGRWQGPLVVAPGSVVLCFGLGSLRDDLVTEVLVRLLRDVGIDARHLSVSDLEAGPP
                     PGASPDSIALLYVVSAFPNAEWDRAAAVVAELRRRFAGRPVVGLLPEEDLESAGDIVD
                     GVAHSFEDALAHAREHFPEATARRKGKAADRPAAAAP"
BASE COUNT          323 a          801 c          744 g          221 t
ORIGIN      
        1 gcgcgcgatg ccggcacgct cgccttccac acggcggcga tcgtcctctc gccggcggtg
       61 ctggccgggc tcgcgcgccg ccggcgctga ccgcccgtcg ctcagggcgc ggccgccgcc
      121 ggccgatctg cggccttgcc cttgcgccgg gccgtggcct cggggaagtg ctcgcgcgcg
      181 tgcgccagcg cgtcctcgaa cgaatgggcg acgccgtcga cgatgtcgcc cgcgctctcg
      241 aggtcctctt cgggcagcag gcccaccacg ggccggccgg cgaagcggcg ccgcagctcg
      301 gcgaccaccg cggccgcgcg atcccattcg gcgttcggga acgcgctcac gacgtacagc
      361 agcgcgatgc tgtccggcga cgcgcccggc ggcgggccgg cctcgaggtc ggagaccgac
      421 aggtgccgcg cgtcgatgcc gacgtcccgc agcaggcgca ccagcacctc ggtgacgagg
      481 tcgtcgcgca gcgagccgag cccgaagcac agcacgacgg agcccggcgc caccacgagc
      541 ggcccctgcc agcgcccgag cgcgctctcg cgccgatggc gcaggtgggc gccgaggctg
      601 ggctcgtcca gcaccgagat gcgctggcgg ccacgcgtgc atccctgcgc ggtcagcgcc
      661 tcgatcacct gcgcgatcgt gcccttcacg cgcatctgct gctcgccgct gatgctgccg
      721 ttcaggaagt cggcgacggc aaggcgcagc gccggcatca ggatgcggtc gcagtagcgc
      781 gcgagcgact tgcgcttgag gtagttgcgc gccgcggcga cgacctcgtc ggcgtccccc
      841 gagagcgcgc gctggtagaa gcgctcggac atcgtgagcg cgggcgtgtc acccagcagg
      901 atgtcgagga acgcgagcga tttcacgtag cgaccgagca ccacgaggca cagcgtcagc
      961 ggcgtcgaga ccagcaggcc gatcgggccc cagatccagc tccagaagat cgccgcgagc
     1021 accaccgaca gcggcgacag ccccgtcgtg tggccgtaca gctgcggctc gacgacctgg
     1081 gacgcgatca gctccaccgc cgcgaacagg cccagcgtga ggaacatcat cgaccagccg
     1141 ggatcgaccg ccgccgccag cagcgccgcg ctgaaggccg ccacgaacac gccgacgtag
     1201 ggcacgaagc gcagcacggc cgtcagcacc gcccacagca tgccgtgcgg caggccgatc
     1261 gccatcgcgc ccagccacac cacggcgccg acgcccacgt tgaccgcgaa ctgcgagacg
     1321 aagaagcgcg acaaccgctc gcccgcgtcg ttgaacgcgt gcgtcgtgcc gcgcaggtcg
     1381 gagccgccga tcaggcggat gaagcgatcg cgcagcgact cgtgctccag cagcacgaag
     1441 acgaggatca cgagcacgat gccggccttg cccagcggcc cccacaccga cgagaacacg
     1501 cgcgacatca cctccaccgg cgtgggcggg cgttcgtgga tctccaccgg cacggcgccg
     1561 ttggcggtca gcggcgcgcg cgcgaggtcg atcgtgccgg gcgcggcgtc ggaggcggcg
     1621 ggcggctggc catccagccg gctcatcatg cggccggcct ccccgcgcat cgcctccatg
     1681 cgcccgagcg tgagctcgcg cacgacctcg accttcgcgc ggatcgtcgt ctcgtactgc
     1741 ggcaggctcg ccgccatcgc gaccacctgc gtcgcgatca cccccgccag cccgaggacg
     1801 cacagcgtcg tgaccgagac cgcgcccagc accgaaggca cctgcccgag ccccagccgc
     1861 cggaaccgcc gcaccagcgg cgcgagcagc aggctcagga tcgccgccag cgtaaccggc
     1921 accagcacct cgcgcccgaa atacagcagc accagcacgg cggtgccgtt gacgagcggg
     1981 cccgcgaagt tgcgcatccc gggctgcagc gccggcggcg gcgcggccgg cggagccagg
     2041 cgcgcggcgg cggacagcgg cgggatcggg tcgatgggcg gcggggcgg
//
LOCUS       DASVLN010000837        16561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_313488, whole genome shotgun sequence.
ACCESSION   DASVLN010000837 DASVLN010000000
VERSION     DASVLN010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 16561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16561
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313488"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VF453_19725"
     CDS             <1..762
                     /locus_tag="VF453_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7689945.1"
                     /translation="DLAQRLADVHDAERLLRATQAELIQAGKLGMLGQMAAGVTHELN
                     QPLTAMQVFADNASALLERGDAAAARVNLGHIADAAGRMGALVAQLKTFARKSDGAPG
                     PVDLARAIDNAALLVRRDFEHAAATLDIDIGQPAVVTGDAIRVEQVLINLLRNALDAV
                     RPCPERRVRVALRAGERAVLRISDTGPGISPLARAHLFEPFFTTKPSGAGLGLGLAIS
                     SSIVQAMGGTLAAEENPGGGAVFVLALPLQEAVVP"
     gene            759..2078
                     /locus_tag="VF453_19730"
     CDS             759..2078
                     /locus_tag="VF453_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7689946.1"
                     /translation="MTIANPEVLLVEDEASVRLATAQALALAGIAVEAFASAEDALAA
                     VGTGFPGVVLSDVRLPGMSGMDLLDEAGRRDRELPVILVTGHGDVELAVQAMRRGAYD
                     FIEKPFRAERLVDVVRRALERRRLVLENRALRLAASERDALPQIVGESGAMQRLRGFV
                     ATIGPTGADVLINGETGTGKEVVARQLHAAGSPGAPFVAINCAAMPETMFESEVFGHE
                     AGAFTGAHKRRIGKFEFARGGTVFLDEIESMPLALQAKLLRVLQERTLERLGGNEAVR
                     IDCRVVAASKTDLLRLSEEGRFRADLYWRIATVSVQLPALREHASDIPQLLAHFVGLA
                     CARYKRPLPDWTPAQMADWSARRWPGNVRELKAFADRLVLGVDDDAGAPPDPQAATLP
                     RQLEAFERRVLRAALAQADGNVALAAERLGVAKTTLYDKLKRFGLGN"
     gene            2193..3737
                     /locus_tag="VF453_19735"
     CDS             2193..3737
                     /locus_tag="VF453_19735"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7689947.1"
                     /translation="MFKDLRIRTRLALGFGGVLLLTVLAIAAGLLEVASVAEQNRRLL
                     DEPLTKERITSDWYRVIDAGSKRTLAMAVSADPVLPATFADDMKSSTALSNKYQDELT
                     KLATSAEEGRRMADILAARKVYLNLRNEIAALRRDGKGDEANAAAERLKPATTGYLGA
                     LQAMLDTERSAIDTRAGAIRDEVARSLWILGTLGVAVLVMGAGFCLFIARGITGPIGD
                     AIAAARRVAAGDVAHDIRVDRRDELGQLQSSMAEMSGQLRRMIAAIRDSASVIAGASQ
                     EIANGNADLSQRTEEQAASLQRTAGSVEQLSAAVRTNADSSAQALALVNSATKSASEG
                     DAIVAEVVSTMRRITDSSTRIADIVGVIDGIAFQTNVLALNASVEAARAGEQGRGFAV
                     VASEVRTLAQRSAAAAKEVRALIDESSGSVDAGAQLVGQAGESMQGILASVRKVDAII
                     GDIAVASREQAGGIEEVNQAVAALDATTQRNAALVEESAAAAMSLQEQSRRLVEAVAV
                     FATQED"
     gene            3740..4849
                     /locus_tag="VF453_19740"
     CDS             3740..4849
                     /locus_tag="VF453_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7689948.1"
                     /translation="MRSGFLAAAAAALLWALAAPARADLVIGQTAGFTGPASGSVKEL
                     AAGAQLVVDDANRHGGVHGEPVKLVQLDDRFDPAQAARNAQALVDQGAIALFASRGTP
                     HTQAILPVLARAHVALVAPSTGARVLYEPVDPYVFTVRAPYQREAEEAIVHLDTVGVT
                     RIAVLHANDAFGADAVAGASKGFTRIGKSPAILAALDRDHPDFAALAPRIAAEQVQAV
                     LIIGTADAVVAGTAAIRAAGSRAQVVTLSNNASQAFVRAMGANARGTVVSQVFPSERA
                     LGVPLVKEAHDLARARGLALTPSMIEGFVGAKALLEALRRAGPAPTRARVLAALDGLS
                     RYDVGGMTLSYSPASHAGLDYVDLSIIGADGQFQR"
     gene            complement(4896..6092)
                     /locus_tag="VF453_19745"
     CDS             complement(4896..6092)
                     /locus_tag="VF453_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HEX7689949.1"
                     /translation="MSLDHPVLNQVVLGASTLFDVKRAPYAARLTVFPQRPDAPVDAQ
                     GLLSVIDEIAPAEAGPLMLSIAHEGALRAVLAAPTASHLMIEVPGFMLADPGLATLLE
                     ERVATGLSLVLGGRVPEGAPAQLWALFRHVTVDRAHVRDPLPNGSGGKPLQRLFIGSD
                     GPVMSAGALKAGFTAVVGWPVGEAPGADSKPKQVPTGVTTVVDLMQRVDREEPAEKLE
                     AVVRNDPTLAFRLMRYLNSPGFGLSVEISSFRHALMILGYARLKRWLALLVTSAIDDP
                     DLKPLMHLAVRRGLLMEEIARPGGDDALRNELFICGVFSTLDRMLGQPFERLLKSIPV
                     QETVAQALTEDAGPCAPFLAIARAVEQESAYDIRESAEALMLTVGDVNRALAKSLVHA
                     HSLAAG"
     gene            complement(6157..7359)
                     /gene="flgL"
                     /locus_tag="VF453_19750"
     CDS             complement(6157..7359)
                     /gene="flgL"
                     /locus_tag="VF453_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEX7689950.1"
                     /translation="MSVDRIATASAYDGAVNNLLERQNNLQQTQIQMTSGKRVVKASD
                     DPTAAARAERARALEERTTASEKAVDASSNAMTLTESALGDASDLLQQARELVVSAGD
                     ASYSDDERASKAAAIAAIRDQLLSVANRPDGQGGYVFSGQGSGGAPFVDEPGGVKFTG
                     VGGSVNVATDEPLPLTLDGGDVWLSASTGNGVFVTQNVNSGGAWIDAGHVTAPNQITG
                     SSYQVVFSTAGGAPTYSILKDGNPTAVSGAPFASGKEIDVDGMSFTISGAPADGDTFQ
                     VTPSAKDLSLFDTLDNVVKSLTTKGKGDAGVAQAVQDGLRDIDQVSAHISSARSYAGT
                     TLQRLDGVKGRLDATQLSAQTSRSDAEDLDMVKALSSFNSQQTGYSAALQSYATIQKM
                     SLFQYLNG"
     gene            complement(7385..9400)
                     /gene="flgK"
                     /locus_tag="VF453_19755"
     CDS             complement(7385..9400)
                     /gene="flgK"
                     /locus_tag="VF453_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX7689951.1"
                     /translation="MSSSALMSIGMRAMAANYASMQTTGHNIANAQTPGYSRQTVDLA
                     SSGGQDTGAGFIGKGVDVVTVQRASDRFLSQQAASASSMAAMDDARASNLKQLETIFP
                     TGDTGVGAAMGNFMNAYVDLANSPSDSSARQVILSDASEVADRFATAGQSLDALQGGV
                     TQDLQSSVAQVNSLAKQIAQINGQIAALRGSASAPNDLLDKRDQLVKQVGNLVKVSTI
                     TASDGSVGVFVAGGQRLVLGAEAESLAVTPDPANTNRSGISISDSGILHPLPNDLLVG
                     GSIAGLLKYQNEDLVAARNQLGQMAAAFASRVNEVQGYGIDMSQPASAGDPIFATGAP
                     AAIANVHNARDAGGALASTCTLKVVDGTQLQASDYTLAADAAVDGNYVVTRQSDGAKF
                     DMLSDGNGGFTYTRQSDGAALGDSLDGFQITLGGAPLGASDSFELQPVGRAAVGMVRV
                     LDDPSGIAASSPETAVMGVANTGTASVDALDVVSSTNDPNTTAAITFTSNAGDYSYTL
                     TDGSGNTTTGTGTWAAGQPIALNGFELTLNGVPNAGDTVSVSRTAHPESNNGNALAMV
                     ALRDETFVGRTKQVDGSLADGVTATDAYAATLANIGVRVQGAKSASDISQAASQQADQ
                     AVDAKSGVNLDEEAARLLQFQQGYQAAAKILQTAQTVFDTLLQVAGR"
     gene            complement(9461..10351)
                     /gene="flgJ"
                     /locus_tag="VF453_19760"
     CDS             complement(9461..10351)
                     /gene="flgJ"
                     /locus_tag="VF453_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEX7689952.1"
                     /translation="MSNSLTASAAQPASLNLGGGLEDLRVKAQRDPKAAVREAAKQFE
                     TLFTQQLLKSMRESTMANADGMDDEGSKMGKEMLDSQWATKLAGQPGGLSDMIAKQLE
                     RQMGLDQGVTTIKPVTANPMPVDPLHPDATVTLPTKAAAAFVSAHTEAANAAEKATGI
                     PATFMIAQAAHETGWGRKEIRNADGTTSYNLFGIKAGPNWKGATTDVTTTEYYGGRPH
                     KVTQTFRAYSSYAESFADYAATMKNSPRYAAVVAAGKDATGFARGLQHAGYATDPAYA
                     DKIAKVIHTTRRVQQQLALA"
     gene            complement(10368..11432)
                     /locus_tag="VF453_19765"
     CDS             complement(10368..11432)
                     /locus_tag="VF453_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX7689953.1"
                     /translation="MLLAAPAQAMRLKEVAAVQGVRSNQLIGYGLVVGLDGTGDQTTQ
                     TPYTTQGLQSMLQQMGVTLPPGTTMQPKNVAAVIVTASLPPFAQPGQTIDIDVSSVAN
                     AKSLRGGTLIATPLKGADGQVYAMAQGNLIVGGAGAAAGGSKVTINHLSAGRIPEGAT
                     VERSVPTPLSQGDQLQLDLNAADFATAHAVAQAINASKGAGTARAVDGRVVMVRAPSD
                     PDARVGFMADIENLDIAQATPAAKVVLNARTGSIVMNQAVSLSPCAVAHGSLSVTINA
                     NPQVSQPAPNSAGETVVTQKTDIAIASQGGSLVNMPAGTRLADVVKALNALGATPQDL
                     LAILQAMKSAGALNADLEVI"
     gene            complement(11554..12336)
                     /gene="flgG"
                     /locus_tag="VF453_19770"
     CDS             complement(11554..12336)
                     /gene="flgG"
                     /locus_tag="VF453_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX7689954.1"
                     /translation="MLRSLWISKTGMEAQQLQLDNISHNLANVATTGYKKSHATFEDL
                     MYQNLRQSGSASSDQTQLPTGLQVGLGVRPVATARDFTQGAMQQTGNSLDVAISGNGF
                     LQVTLPDGTTGYTRDGSLKMDANGQIVTSNGYTVQPGITIPAQAQSISIAADGTVSAT
                     LPGNATPSTLGQLQLASFANPAGLDPKGENIYTETASSGSPSTAAPGTNGLGRLQQGF
                     LESSNVNVVEELVSMISTQRAYELNSKAIQTSDQMLQRLGQL"
     gene            complement(12578..13306)
                     /gene="flgF"
                     /locus_tag="VF453_19775"
     CDS             complement(12578..13306)
                     /gene="flgF"
                     /locus_tag="VF453_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEX7689955.1"
                     /translation="MDRMIYTSMSGAKAALQRQDTLANNLANVSTNGFRAELTAFRAV
                     PIRGDGASTRVFALESTPGYNDAQGPITATGRSLDVAMTGNSWLAVQGLDGTEAYTRQ
                     GSLQINSEGVLVTENGLQVNGDGGPITIPPNASVDIAADGTVSARVGNQKPTNVGKLK
                     IVTPESPMTRGTDGLFRADSGDLSADPAARIQSGALEGSNVSPVETMVGMIAAARQFE
                     HQMKLLEGAEKQDQAASKLLSENG"
     gene            complement(13338..14606)
                     /gene="flgE"
                     /locus_tag="VF453_19780"
     CDS             complement(13338..14606)
                     /gene="flgE"
                     /locus_tag="VF453_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEX7689956.1"
                     /translation="MSFQQGLSGLNAASKNLEVIGNNVANSNTYGEKSSRAEFADMYA
                     AAMTGAAGNSVQAGIGVQVAAVSQQFTQGNITPTGNPLDLAINGNGFFQVRDSSNQTE
                     YTRNGQFKVDDNGNIVNDQGNKLLGYAADAQGNVVSGAAVPLQLPTGGIDPAATSKVT
                     MEFNLDSRSGVTVPASGPAIDFTDQKTYNNATSVTMFDAKGQDVAVSYYFQKSGNDTW
                     NVFATANGTTVGGTAGAPAPIAQLQYASDGSTLVSPTGPISLDIPASTNAAGATTLPI
                     SGIQLDLSGATEFGSNFGVTNMVQNGYAAGGLTGVSVDTTGKLIAQYSNGQTKAAGQL
                     ELATFRNPQGLAAQGGNSWKQTSASGGPIVGVAGDGNLGSLDAGSLEESNVDLTAQLV
                     QMITAQRDYQANAQTIKTMDQAMQTLVNLR"
     gene            complement(14626..15381)
                     /locus_tag="VF453_19785"
     CDS             complement(14626..15381)
                     /locus_tag="VF453_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEX7689957.1"
                     /translation="MSTTAMNPLSATSGIPGLPPMPVAATAAKAKAAAAAGASSGTAA
                     AGDANKALGPTSSAADQSNRFLKLLVAQMSNQDPLNPMDSAQVTSQMAQISTVEGVQT
                     LNTTVSGLNGQFAQLQAMQGASLVGREVAIAGNSVHVDADTHQADGGFQIDTPATDVQ
                     VDILNPAGQSVGTVRLGAQDAGRHSFSWDVPEAQRGQALTFKVTAMNNASEVDSKPLA
                     YGGISAVSTAGGKLSLELDNGKSVGYDDVQAFL"
     gene            complement(15401..15817)
                     /gene="flgC"
                     /locus_tag="VF453_19790"
     CDS             complement(15401..15817)
                     /gene="flgC"
                     /locus_tag="VF453_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX7689958.1"
                     /translation="MSLFKIFDISGSAVSSQSQRLNVVASNLANVDTVAGPDGKAYKA
                     RQVTFQTVLMNTPDGVPGAGVKVSNITEDQTPGRRVHDPSSPSADADGYVTYSNVNAV
                     DEMVNMISASRSYQNNIEVMNTAKNLLMKTLQMGQG"
     gene            complement(15830..16249)
                     /gene="flgB"
                     /locus_tag="VF453_19795"
     CDS             complement(15830..16249)
                     /gene="flgB"
                     /locus_tag="VF453_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX7689959.1"
                     /translation="MPRMTDSLDFQAQALTLRAERQRIIAGNIANADTPGFVARDMDF
                     ASALREATGQLSGAQSMSGSAMTNAGHLPLTAESTSALALKYSAPSQTSLDSNTVDMD
                     RERASFVDNSVKYEATLRFINTQVRTMLDAMKSPSQG"
BASE COUNT         2105 a         5691 c         6137 g         2628 t
ORIGIN      
        1 gacctcgcgc agcgcctggc cgacgtgcac gacgccgagc gcctgctgcg cgcgacccag
       61 gccgagctga tccaggccgg caagctgggc atgctcgggc agatggccgc cggcgtcacg
      121 cacgagctca accagccgct gaccgcgatg caggtgttcg cggacaacgc gagcgccctc
      181 ctcgagcgcg gcgacgccgc ggccgcgcgc gtgaacctcg gccacatcgc cgacgccgcc
      241 ggccgcatgg gcgcgctcgt cgcgcagctc aagaccttcg cgcgcaagtc cgacggcgcg
      301 cccgggccgg tcgacctcgc gagggccatc gacaacgcgg ccctgctcgt gcggcgcgac
      361 ttcgagcacg ccgccgcgac gctggacatc gacatcgggc agccggccgt cgtcaccggc
      421 gacgcgatcc gggtcgagca ggtgctgatc aacctgctgc gcaacgcgct cgacgccgtg
      481 cgaccctgcc ccgagcgtcg cgtgcgcgtg gcgctgcggg ccggcgagcg cgccgtgctg
      541 cggatctccg ataccggacc gggtatctcg ccgctcgccc gggcgcacct gttcgagccg
      601 ttcttcacca ccaagccctc gggcgccggg ctgggcctgg ggctcgccat ctcgtcgtcc
      661 atcgtgcagg cgatgggcgg cacgctcgcg gccgaggaga acccgggcgg cggcgccgtg
      721 ttcgtgctcg cgctgccgct gcaggaagcc gtcgtgccat gaccatcgcg aatcccgaag
      781 tgctgctggt cgaggacgag gcgagcgtgc ggctcgcgac ggcgcaggcg ctcgcgctcg
      841 cgggcatcgc cgtcgaggcc ttcgcgagcg ccgaggacgc gctggcggcc gtcggcacgg
      901 gctttcccgg cgtcgtgctg agcgacgttc ggctgccggg gatgtccggc atggacctgc
      961 tcgacgaggc agggcggcgc gaccgcgagc tgcccgtcat cctcgtgacc ggccacggcg
     1021 acgtcgagct cgcggtgcag gcgatgcggc gcggcgcgta cgacttcatc gagaagccgt
     1081 tccgcgccga gcgcctcgtc gacgtcgtgc ggcgcgcgct ggagcgccgc cgcctcgtcc
     1141 tcgagaaccg cgcgctgcga ctggccgcga gcgagcgcga cgcgctgccg cagatcgtcg
     1201 gcgaatcggg cgcgatgcag cgcctgcgcg gcttcgtcgc cacgatcggc ccgaccggcg
     1261 cggacgtgct gatcaacggc gagaccggca ccggcaagga agtcgtcgcg cgccagctgc
     1321 acgccgcggg ctcgccgggc gcgcccttcg tcgcgatcaa ctgcgccgcg atgccggaga
     1381 cgatgttcga gagcgaggtc ttcggccacg aggccggggc gttcaccggc gcgcacaagc
     1441 gccgcatcgg caagttcgag ttcgcgcgcg gcggcaccgt gttcctcgac gagatcgagt
     1501 cgatgccgct cgccctgcag gccaagctgc tgcgcgtgct gcaggaacgc acgctcgagc
     1561 gcctgggcgg caacgaggcc gtgcgcatcg actgccgcgt ggtcgcggcc agcaagaccg
     1621 acctgctcag gctgtccgag gaaggccgct tccgcgcgga cctgtactgg cgcatcgcca
     1681 ccgtctcggt gcagctgccg gcgctgcgcg agcacgcctc ggacatcccg cagctgctcg
     1741 cccacttcgt cggcctcgcc tgcgcgcgct acaagcggcc gctgcccgac tggacgcccg
     1801 cgcagatggc cgactggtcg gcgcgccgct ggcccggcaa cgtgcgcgag ctcaaggcct
     1861 tcgccgaccg cctggtgctg ggcgtggacg acgatgccgg cgcgccgccc gatccgcagg
     1921 ccgcgacgct cccgcgccag ctcgaggcct tcgagcgccg cgtgctgcgt gccgcgctcg
     1981 cgcaggccga cggcaacgtc gcgctcgcgg cggagcgcct gggcgtggcc aagaccacgc
     2041 tgtacgacaa gctcaagcgc ttcggactgg gcaactgagt gatcgatttc acgccggccg
     2101 gtgcgatccc ccgatccagc cggccggcgc gccgccgatc tgagtggagg gcacccggcc
     2161 ccttcagccg ggcacgacag gagacaaaac gcatgttcaa ggatctgcgc atccgcaccc
     2221 gcctggccct cggcttcggc ggcgtgctcc tgctgacggt gctcgcgatc gccgccggcc
     2281 tgctggaggt ggcctcggtc gccgagcaga accggcgcct gctcgacgag ccgctgacca
     2341 aggagcgcat caccagcgac tggtaccgcg tgatcgacgc gggcagcaag cgcacgctgg
     2401 cgatggcggt cagcgccgac ccggtgctgc cggccacctt cgcggacgac atgaagtcct
     2461 cgacggcgct gtccaacaag taccaggacg aactcaccaa gctcgcaact tccgccgagg
     2521 aaggccggcg catggccgac atcctggccg cgcgcaaggt ctacctgaac ctgcgcaacg
     2581 agatcgccgc cctgcgccgc gacggcaagg gcgacgaggc caacgccgcc gccgagcgcc
     2641 tgaagccggc gacgacgggc tacctgggcg cgctgcaggc gatgctcgac accgagcgct
     2701 cggccatcga cacccgcgcc ggcgcgatcc gcgacgaggt cgcgcgcagc ctgtggatcc
     2761 tgggcacgct cggcgtggcg gtgctcgtca tgggcgccgg attctgcctg ttcatcgcgc
     2821 gcggcatcac gggcccgatc ggcgacgcga tcgccgcggc ccggcgcgtg gcggcgggcg
     2881 acgtcgccca cgacatccgc gtcgaccgcc gcgacgaact cggccagctg cagtcgtcga
     2941 tggcggagat gtcgggccag ctgcggcgca tgatcgcggc gatccgcgac agcgcgtcgg
     3001 tgatcgccgg cgcgtcgcag gagatcgcca acggcaacgc cgacctgtcg cagcgcaccg
     3061 aggagcaggc cgcctcgctg cagcgcacgg ccggcagcgt cgagcagctg agcgccgcgg
     3121 tgcgcaccaa cgccgactcg agcgcgcagg cgctggcgct cgtcaattcg gcgacgaagt
     3181 cggccagcga gggcgacgcg atcgtcgccg aggtcgtcag cacgatgcgc cgcatcaccg
     3241 actcgtcgac ccgcatcgcc gacatcgtgg gcgtcatcga cggcatcgcg ttccagacca
     3301 acgtgctcgc gctgaacgcc tccgtcgagg cggcgcgcgc gggcgagcag ggccgcggct
     3361 tcgcggtcgt cgcctccgag gtgcgcacgc tggcacagcg cagcgccgcg gcggccaagg
     3421 aggtgcgcgc gctgatcgac gagtcctcgg gcagcgtgga tgcgggcgcg cagctggtcg
     3481 ggcaggccgg cgagtcgatg cagggcatcc tcgcgtcggt gcgcaaggtc gacgccatca
     3541 tcggcgacat cgcggtcgcg agccgcgagc aggccggggg catcgaggag gtcaaccagg
     3601 ccgtcgccgc gctggacgcg acgacgcagc gcaacgccgc gctcgtcgag gaatcggcgg
     3661 cggcggcgat gtcgctgcag gagcagtcgc gccgcctcgt cgaggcggtg gccgtgttcg
     3721 cgacgcagga ggactgagca tgcgatccgg cttcctcgcg gcggccgccg cggcgctgct
     3781 gtgggcgctc gcggcgccgg cgcgcgccga tctcgtcatc ggccagaccg ccggcttcac
     3841 gggccccgcc tcgggcagcg tcaaggagct cgcggccggc gcgcagctgg tcgtcgacga
     3901 cgcgaaccgc cacggcggcg tgcacggcga gccggtgaag ctggtgcagc tggacgaccg
     3961 cttcgacccc gctcaggcgg cgcgcaacgc gcaggcgctg gtcgaccagg gcgcgatcgc
     4021 cctgttcgcc tcgcgcggca cgccgcacac gcaagcgatc ctgccggtgc tggcgcgtgc
     4081 gcacgttgcg ctggtcgcgc cgtcgaccgg cgcccgcgtg ctgtacgaac ccgtcgaccc
     4141 gtacgtcttc accgtgcgcg cgccttacca gcgcgaggcc gaggaggcga tcgtccacct
     4201 ggacaccgtc ggcgtcacgc gcatcgccgt gctgcacgcc aacgacgcgt tcggcgccga
     4261 cgcggtggcg ggcgcgagca agggcttcac gcgcatcggc aagtcgccgg cgatcctggc
     4321 ggcgctcgac cgcgaccatc ccgacttcgc ggcgctcgcc ccgcgcatcg ccgccgagca
     4381 ggtgcaggcg gtgctgatca tcggcacggc ggacgccgtc gtcgcgggca ccgccgcgat
     4441 ccgcgccgcg ggctcgcgcg cgcaggtggt cacgctctcg aacaacgcct cgcaggcctt
     4501 cgtgcgcgcg atgggcgcca acgcgcgcgg gacggtggtc tcgcaggtgt ttccgtcgga
     4561 gcgcgcgctc ggcgtgccgc tggtcaagga ggcgcacgac ctggcccggg cgcgcgggct
     4621 cgcgctcacg ccgtcgatga tcgagggctt cgtcggcgcg aaggcgctgc tcgaggccct
     4681 gcgccgcgcc gggccggcgc ccacgcgcgc ccgcgtgctc gcggcgctcg acggcctgtc
     4741 gcgctacgac gtgggcggca tgacgctgtc gtactcgccc gccagccatg cggggctgga
     4801 ctacgtcgac ctgtccatca tcggcgcgga cgggcagttc cagcgctgac ccgcgcccga
     4861 cctcgctgcg ccgtcattgc gccgtcatca ggtcgtcagc cggccgcgag cgagtgggca
     4921 tgcaccagcg acttcgcgag cgcccggttc acgtcgccga cggtcagcat cagcgcctcc
     4981 gcggactcgc ggatgtcgta ggccgactcc tgctccacgg cgcgcgcgat cgcgaggaag
     5041 ggcgcgcacg ggccggcatc ctcggtgagc gcctgggcga cggtctcctg caccgggatg
     5101 ctcttgagca ggcgctcgaa aggctggccc agcatgcggt cgagggtgga gaacacgccg
     5161 cagatgaaca gctcgttgcg cagcgcgtcg tcgccgccgg ggcgggcgat ctcctccatc
     5221 agcaggccgc ggcgcacggc caggtgcatc agcggcttga ggtcggggtc gtcgatggcg
     5281 ctggtgacca gcagcgcgag ccagcgcttg agccgcgcgt agccgaggat catcaacgcg
     5341 tggcggaagg acgagatctc caccgacagc ccgaagcccg gcgagttgag gtagcgcatc
     5401 aggcggaacg cgagcgtcgg gtcgttcctg accaccgcct ccagcttttc cgccggctcc
     5461 tcgcggtcga cgcgctgcat caggtcgacg accgtggtga cgccggtggg cacctgcttg
     5521 ggcttgctgt cggccccggg cgcctcgccg accggccagc cgacgaccgc cgtgaatccc
     5581 gccttgagcg cgccggccga catcaccggg ccgtccgagc cgatgaacag gcgctgcagc
     5641 ggcttgccgc cggagccgtt cgggagcggg tcgcgcacgt gcgcgcggtc gacggtcacg
     5701 tggcggaaca gcgcccacag ctgcgccggc gcgccctcgg gcacgcggcc gccgagcacg
     5761 agcgacaggc cggtggccac gcgctcctcc agcagcgtcg ccaggcccgg gtcggcgagc
     5821 atgaagccgg gcacctcgat catcaggtgg gacgcggtcg gcgccgcgag caccgcgcgc
     5881 agcgcgcctt cgtgggcgat gctgagcatc agcgggcccg cctcggcggg cgcgatctcg
     5941 tcgatcaccg agagcaggcc ctgcgcgtcg accggcgcgt cgggccgttg cgggaagacc
     6001 gtcagccgcg ccgcgtacgg cgcgcgcttg acgtcgaaca gcgtgctcgc gccgagcacg
     6061 acctggttca gtaccgggtg gtcgagggac atgtcgggat cctctggggc atgggtggat
     6121 ggcgcgatgg aacggacggc cgcggcggcc gcagggtcag ccgttcaggt actggaacag
     6181 cgacatcttc tggatcgtcg catagctctg gagcgccgcc gaataacccg tctgctggga
     6241 gttgaacgag gacagcgcct tgaccatgtc gaggtcttcg gcatccgagc gcgaggtctg
     6301 agcggacagc tgcgtcgcgt cgagccggcc cttgacgccg tccaggcgct gcagggtcgt
     6361 gccggcgtag ctgcgcgccg aggagatgtg ggccgagacc tggtcgatgt cgcgcaggcc
     6421 atcctgtacc gcctgggcca cgccggcgtc gcccttgccc ttcgtggtca ggctcttgac
     6481 gacgttgtcc agggtgtcga acagcgacag gtccttcgcg gagggcgtca cctggaaggt
     6541 gtcgccgtcg gccggggcgc cgctgatcgt gaaggacatg ccgtcgacgt cgatctcctt
     6601 gccgctcgcg aacggggcgc ccgagacggc ggtcgggttg ccgtccttca ggatcgagta
     6661 ggtcggcgcg ccgccggcgg tgctgaagac gacctggtag ctggagccgg tgatctggtt
     6721 gggcgccgtc acgtgcccgg cgtcgatcca ggcgccgccg gagttcacgt tctgcgtcac
     6781 gaacacgccg ttgccggtgc tggccgagag ccacacgtcg ccgccgtcca gcgtgagcgg
     6841 cagcggctcg tcggtggcca cgttgacgct gccgccgacg ccggtgaact tcacgccgcc
     6901 gggctcgtcg acgaagggcg cgccgcccga gccctggccg gagaagacgt agccgccctg
     6961 gccgtcgggc cggttggcca ccgacagcag ctggtcgcgg atcgccgcga tcgccgccgc
     7021 cttggaggcg cgctcgtcgt cgctgtagct cgcgtcgccg gcgctcacga ccagctcgcg
     7081 cgcctgctgc agcaggtcgg aggcgtcgcc cagcgcggac tcggtgagcg tcatggcgtt
     7141 gctgctggcg tccaccgcct tctcgctggc ggtggtgcgt tcctcgagcg cgcgggcgcg
     7201 ttccgcgcgc gcggcggcgg tcgggtcgtc gctcgccttg acgacgcgct tgccgctggt
     7261 catctggatc tgcgtctgct gcaggttgtt ctgccgctcc agcaggttgt tgaccgcgcc
     7321 gtcgtaggcg ctggcggtcg cgatgcgatc cacgctcatg ggatgtccct gcctctatct
     7381 cgaatcaacg ccccgccacc tgcagcaacg tgtcgaacac ggtctgcgcg gtctgcagga
     7441 tcttggcggc ggcctggtag ccctgctgga actgcagcag gcgcgcggcc tcctcgtcga
     7501 ggttcacgcc gctcttggcg tcgaccgcct ggtcggcctg ctgcgaggcg gcctgcgaga
     7561 tgtccgacgc cgacttggca ccctgcacgc gcacgccgat gttggcgagc gtcgcggcgt
     7621 aggcgtcggt ggcggtcacg ccgtcggcga gcgagccgtc gacctgcttc gtgcggccga
     7681 cgaaggtctc gtcgcgcagc gcgaccatcg cgagcgcgtt gccgttgttg ctctccgggt
     7741 gcgcggtccg ggagacgctg accgtgtcgc ccgcgttcgg cacgccgttg agcgtcagct
     7801 cgaagccgtt gagcgcgatc ggctggccgg ccgcccacgt gccggtgccg gtggtcgtgt
     7861 tgccgctgcc gtcggtgagc gtgtagctgt agtcgcccgc gttggacgtg aaggtgatcg
     7921 ccgccgtcgt gttgggatcg ttggtgctgc tgacgacgtc cagcgcatcg acgctggcgg
     7981 tgcccgtgtt ggccacgccc atcacggcgg tctcgggcga cgaggcggcg atgcccgacg
     8041 ggtcgtcgag cacgcgcacc atgcccaccg cggcgcggcc gaccggctgc agctcgaagc
     8101 tgtcgctggc ccccagcggc gcgccaccca gcgtgatctg gaagccgtcc aggctgtcgc
     8161 cgagcgccgc gccgtcggac tggcgcgtgt aggtgaagcc gccgttgccg tcggacagca
     8221 tgtcgaactt cgcgccgtcg gactgccgcg tgacgacgta gttgccgtcg accgccgcgt
     8281 cggcggccag cgtgtagtcg ctcgcctgca gctgcgtgcc gtcgacgacc ttgagcgtgc
     8341 aggtgctcgc gagcgcgccg cccgcgtcgc gcgcgttgtg gacgttggcg atcgccgccg
     8401 gcgcgccggt cgcgaagatc gggtcgccgg cgctggcggg ctggctcatg tcgatgccgt
     8461 agccctgcac ctcgttgacg cgcgacgcga acgccgccgc catctgcccg agctggttgc
     8521 gggcggcgac gagatcctcg ttctggtact tcagcaggcc ggcgatcgag ccgcccacga
     8581 gcaggtcgtt gggcagcggg tgcaggatgc cgctgtcgct gatcgagatg cccgagcggt
     8641 tcgtgttggc cgggtcgggc gtgacggcga gcgactcggc ctccgcgccc agcacgaggc
     8701 gctggccgcc ggcgacgaac acgcccaccg agccgtcgct ggccgtgatc gtcgagacct
     8761 tgacgaggtt gccgacctgc ttgaccagct ggtcgcgctt gtcgagcagg tcgttgggcg
     8821 ccgaggccga gccgcgcagc gcggcgatct ggccgttgat ctgcgcgatc tgcttggcca
     8881 gcgagttgac ctgcgcgacc gagctctgga ggtcctgcgt gacgccgccc tgcagcgcgt
     8941 cgagcgactg gccggcggtc gcgaagcggt cggcgacctc gctcgcgtcg gacaggatga
     9001 cctgccgcgc cgacgagtcc gacgggctgt tggcgaggtc gacgtaggcg ttcatgaagt
     9061 tgcccatcgc cgcgcccacg ccggtgtcgc cggtcgggaa gatcgtctcc agctgcttca
     9121 ggttggaggc gcgcgcgtcg tccatcgcgg ccatcgagct cgccgacgcc gcctgctgcg
     9181 agaggaagcg gtcgctcgcg cgctgcacgg tgacgacgtc cacgcccttg ccgatgaagc
     9241 ccgcgccggt gtcctggccg ccggagctcg cgaggtccac cgtctggcgc gagtagccgg
     9301 gcgtctgcgc gttggcgatg ttgtggcccg tcgtctgcat ggacgcgtag ttggcggcca
     9361 tcgcgcgcat gccgatcgac atcagtgccg acgagctcat cgcatgcctc cttccgtttc
     9421 agtccgcaag tccatggccg atatcccgat cacaaggctc tcaggccagc gccagctgct
     9481 gctgcacgcg gcgggtcgtg tggatgacct tggcgatctt gtccgcgtag gccgggtcgg
     9541 tcgcgtagcc ggcgtgctgc aggccgcgcg cgaagccggt cgcgtccttg cccgccgcga
     9601 cgacggcggc gtagcgcggg ctgttcttca tcgtcgccgc gtagtcggcg aaggactcgg
     9661 catagctcga gtaggcgcgg aaggtctgcg tgaccttgtg cgggcggccg ccgtagtatt
     9721 cggtggtcgt gacgtcggtg gtcgcgccct tccagttcgg gccggccttg atgccgaaca
     9781 ggttgtacga ggtcgtgccg tccgcgttgc ggatctcctt gcggccccag ccggtctcgt
     9841 gcgcggcctg cgcgatcatg aaggtcgccg ggatgccggt ggccttctcg gcggcgttgg
     9901 ccgcctcggt gtgcgccgag acgaatgccg ccgcggcctt ggtcggcagc gtgacggtgg
     9961 cgtccgggtg cagcgggtcg accggcatcg ggttggcggt caccggcttg atcgtggtga
    10021 cgccctggtc gaggcccatc tggcgctcga gctgcttggc gatcatgtcc gagagcccgc
    10081 ccggctggcc ggcgagcttg gtcgcccatt gcgagtccag catctccttg cccatcttcg
    10141 agccctcgtc gtccatgccg tcggcgttgg ccatggtgga ctcgcgcatg ctcttgagca
    10201 actgctgggt gaacagcgtc tcgaactgct tggcggcctc gcgcacggcg gccttcgggt
    10261 cgcgctgtgc cttgacgcgc aggtcctcca gcccaccgcc gaggttcagc gaggcgggtt
    10321 gcgcggcgga ggcggtgagg gaattgctca tggcgcaggc cttcgcgtca gatcacttcc
    10381 aggtcggcgt tgagcgcgcc ggccgacttc atggcctgca ggatcgcgag caggtcctgc
    10441 ggcgtcgcgc ccagcgcgtt gagcgccttg acgacgtctg ccagcctggt gcccgcaggc
    10501 atgttcacga gcgagccgcc ctggctggcg atcgcgatgt cggtcttctg ggtgacgacc
    10561 gtctcgccgg cggagttcgg cgccggctgg ctcacctgcg gattggcgtt gatcgtcacc
    10621 gacaggctgc cgtgcgcgac cgcgcagggg ctcaggctga cggcctggtt catcacgatg
    10681 gagccggtgc gcgcgttgag cacgaccttg gccgcgggcg tggcctgggc gatgtcgagg
    10741 ttctcgatgt cggccatgaa gccgacgcgg gcgtccgggt cggacggcgc gcgcaccatc
    10801 acgacgcggc cgtcgaccgc gcgcgccgtg cccgcgccct tggaggcgtt gatcgcctgg
    10861 gcgaccgcgt gcgcggtggc gaagtcggcg gcgttcaggt cgagctgcag ctggtcgccc
    10921 tgcgacagcg gcgtcggcac cgagcgctcg accgtcgcgc cctccgggat gcggccggcg
    10981 ctcaggtggt tgatcgtgac cttggagccg cccgcggccg cgccggcgcc gccgacgatc
    11041 aggttgccct gcgccatcgc gtagacctgg ccgtcggcgc ccttcagcgg ggtggcgatc
    11101 agcgtgccgc cgcgcaggct cttggcgttg gcgaccgagg agacgtcgat gtcgatcgtc
    11161 tgccccggct gcgcgaacgg cggcagcgaa gcggtgacga tcaccgcggc gacgttcttg
    11221 ggctgcatcg tcgtgcccgg cggcagcgtg acgcccatct gctgcagcat cgactgcagc
    11281 ccctgcgtgg tgtagggcgt ctgcgtggtc tggtcgccgg tgccgtccag gcccacgacc
    11341 aggccgtagc cgatcagctg gttgctgcgc acgccctgca cggccgcgac ctccttgagc
    11401 cgcatggcct gcgccggcgc ggcgagcagc atcgacgccg ccgcgagcag cgaggcgacg
    11461 aagccgaggg tcgagcgggc ggagcggaag gcgagcgtca tggcggcgga tcggtggagc
    11521 gggcgggtcg ggcggatggc gaaggcgtcg tcgtcagagc tggccgaggc gctgcagcat
    11581 ctggtcggag gtctggatgg ccttggagtt gagctcgtag gcgcgctggg tcgagatcat
    11641 cgacaccagc tcctcgacga cgttgacgtt gctgctctcc aggaagccct gctgcaggcg
    11701 gccgaggccg ttggtgcccg gggcggcggt cgagggcgag cccgaggacg cggtctcggt
    11761 gtagatgttc tcgcccttgg ggtcgaggcc ggccgggttg gcgaagctcg cgagctgcag
    11821 ctggcccagc gtcgagggcg tcgcattgcc gggcagcgtc gcggagacgg tgccgtcggc
    11881 ggcgatggag atcgactgcg cctgcgccgg gatggtgatg ccgggctgca cggtgtagcc
    11941 gttgctcgtg acgatctggc cgttggcgtc catcttcagc gagccgtcgc gggtatagcc
    12001 ggtggtgccg tccggcaggg tgacctgcag gaagccgttg ccgctgatgg ccacgtccag
    12061 cgagtttccg gtctgctgca tcgcgccctg cgtgaagtcg cgcgcggtgg cgacggggcg
    12121 cacgccgagg ccgacctgca ggcccgtggg cagctgcgtc tggtcggagc tcgccgagcc
    12181 cgactggcgc aggttctggt acatcaggtc ctcgaaggtc gcgtggctct tcttgtagcc
    12241 ggtggtcgcg acgttggcga ggttgtgcga gatgttgtcc agctggagct gctgggcctc
    12301 catgccggtc ttcgagatcc acagtgaacg gagcatgacc tgattcccaa tgatgggtga
    12361 tggtcagatc atcgcgaaaa cttgagggtc caatcgcccg gataaggccg ccttcccagg
    12421 cgcttatcga agctgtctgc gctcgcttga agcgcgaccg ctccggcgct gccgctcttc
    12481 cgtctcatga tagacacggg gaagcggcag atccggctcc gccggtctgc acgcgcacgc
    12541 cccctcgggg ggcagggagc gctagcgacc gtgggggtca accattttcg ctgaggagct
    12601 tggaggccgc ctggtcctgc ttctcggcgc cttccagcag cttcatctgg tgctcgaact
    12661 ggcgcgcggc ggcgatcatg ccgaccatgg tctcgaccgg gctcacgttg gagccctcga
    12721 gcgcgcccga ctggatgcgc gcggccgggt cggccgagag gtcgcccgag tcggcgcgga
    12781 acagcccgtc cgtgccgcgc gtcatcggtg actcgggcgt gacgatcttg agcttgccga
    12841 cgttggtcgg cttctggttg cccacgcgcg cggagacggt gccgtccgcg gcgatgtcca
    12901 ccgaggcgtt gggcgggatc gtgatcgggc cgccgtcgcc attgacctgc aggccgttct
    12961 cggtgaccag cacgccctcg gagttgatct gcagcgagcc ctggcgcgtg tacgcctcgg
    13021 tgccgtcgag cccctgcacc gcgagccagg aattgccggt catcgcgacg tccaggctgc
    13081 gcccggtcgc ggtgatcggc ccctgggcgt cgttgtagcc gggggtgctc tccagcgcga
    13141 agacgcgcgt ggacgcgccg tcgccgcgga tgggcacggc gcggaacgcc gtcagctcgg
    13201 cccggaagcc gttggtcgag acgttggcga ggttgttggc gagcgtgtcc tggcgctgca
    13261 gcgcggcctt cgcgcccgac atcgaggtgt agatcatgcg atccatggcg acgctccttc
    13321 gctatccgtc cggccggtca gcgcaggttg accagcgtct gcatcgcctg gtccatcgtc
    13381 ttgatggtct gggcgttggc ctggtagtcg cgctgcgcgg tgatcatctg caccagctgg
    13441 gcggtcaggt cgacgttgga ttcctcgagc gagccggcgt cgagcgagcc gaggttgccg
    13501 tcgccggcca cgccgacgat cgggccgccc gaggccgagg tctgcttcca cgagttgccg
    13561 ccctgcgccg cgaggccctg cgggttgcgg aaggtcgcga gctcgagctg gccggccgcc
    13621 ttggtctggc cgttggagta ctgggcgatc agcttgccgg tcgtgtccac cgacacgccc
    13681 gtcaggccgc cggccgcgta gccgttctgc accatgttgg tcacgccgaa gttcgagccg
    13741 aactcggtcg cgccggacag gtcgagctgg atgcccgaga tcggcagcgt cgtcgcgccg
    13801 gccgcgttgg tgctggccgg gatgtccagc gagatcggcc cggtgggcga gacgagcgtg
    13861 ctgccgtcgc tcgcgtactg gagctgggcg atgggcgcgg gcgcccccgc ggtgccgccg
    13921 acggtcgtgc cgttggcggt cgcgaagacg ttccaggtgt cgttgcccga cttctggaag
    13981 tagtagctga ccgcgacgtc ctggcccttg gcgtcgaaca tcgtgaccga ggtcgcgttg
    14041 ttgtaggtct tctggtcggt gaagtcgatc gccgggccgc tcgccggcac cgtcacgccc
    14101 gagcgcgagt cgaggttgaa ctccatcgtg accttgctgg tggccgccgg gtcgatgccg
    14161 ccggtgggca gctgcagcgg cacggccgcg cccgagacca cgttgccctg cgcatcggcc
    14221 gcgtagccga gcagcttgtt gccctggtcg ttgacgatgt tgccgttgtc gtcgaccttg
    14281 aactggccgt tgcgcgtgta ctcggtctgg ttggacgagt cgcgcacctg gaagaagccg
    14341 ttgccgttga tggcgaggtc cagcgggttg cccgtcggcg tgatgttgcc ctgcgtgaac
    14401 tgctgcgaga ccgccgccac ctggacgccg atgcccgcct gcacgctgtt gccggcggcg
    14461 cccgtcatcg cggcggcgta catgtcggcg aactccgcgc gcgaggactt ctcgccgtag
    14521 gtgttggagt tggcgacgtt gttgccgatc acctcgaggt tcttcgaggc ggcgttcagg
    14581 ccggacaggc cctgctggaa gctcatggat gtctcctgga acggatcaga ggaaggcctg
    14641 cacgtcgtcg tagccgacgc tcttgccgtt gtcgagctcg agcgacagct tgccgcccgc
    14701 ggtcgagacg gccgagatgc cgccataggc caggggcttc gaatcgacct cgctggcgtt
    14761 gttcatcgcg gtgaccttga aggtcagcgc ctggccgcgc tgggcctcgg ggacgtccca
    14821 cgagaagctg tggcggccgg cgtcctgcgc gccgaggcgg acggtgccga ccgactgccc
    14881 ggccgggttg aggatgtcga cctgcacgtc ggtggccggc gtgtcgatct ggaagccgcc
    14941 gtcggcctgg tgggtgtcgg cgtcgacgtg caccgagttg ccggcgatcg cgacctcgcg
    15001 acccaccagc gacgcgccct gcatcgcctg cagctgcgcg aactggccgt tcaggccgct
    15061 gacggtcgtg ttcagcgtct gcacgccctc cacggtggag atctgcgcca tctggctcgt
    15121 gacctgtgcg ctgtccatcg ggttgagcgg atcctggttg gacatctgcg cgaccagcag
    15181 cttcaggaag cggttggact ggtcggcggc ggagctggtc gggccgagcg ccttgttggc
    15241 gtcgccggcc gcggccgtgc cgctcgaggc gcctgccgcg gcggcggcct tggccttcgc
    15301 cgcggtggcg gccacgggca tcggcggcag gccggggatg ccggacgtgg ccgacagcgg
    15361 gttcatggcg gtggtggaca tgtgtcgtca gctttcgtgg tcagccctgg cccatctgca
    15421 gcgtcttcat cagcaggttc ttggccgtgt tcatcacttc gatgttgttc tggtacgagc
    15481 gcgaggccga gatcatgttg accatctcgt cgaccgcgtt gacgttggaa taggtgacgt
    15541 agccgtcggc gtccgcgctc ggcgagctcg ggtcgtgcac gcgccggccc ggcgtctggt
    15601 cctccgtgat gttggagacc ttcacgcccg cgcccggcac gccgtcgggc gtgttcatca
    15661 gcacggtctg gaacgtgacc tggcgtgcct tgtaggcctt gccgtccggg cccgcgaccg
    15721 tgtcgacgtt ggcgaggttg gacgcgacga cgttgagccg ctggctctgc gagctgacgg
    15781 cgctgccgga aatgtcgaag atcttgaaca ggctcatggg ttactccgct cagccctggc
    15841 tcgggctctt catcgcgtcg agcatcgtgc gcacctgcgt gttgatgaag cgcagcgtgg
    15901 cctcgtactt gaccgagttg tcgacgaagc tggcgcgctc gcggtccatg tcgaccgtgt
    15961 tgctgtcgag gctggtctgc gacggcgccg aatacttgag cgccagcgcg gaggtcgact
    16021 cggcggtgag cggcaggtgg ccggcgttgg tcatggccga gccgctcatc gattgcgcgc
    16081 ccgacagctg gcccgtcgcc tcccgcagcg cggacgcgaa gtccatgtcg cgggccacga
    16141 agccgggcgt gtcggcgttg gcgatgttgc ccgcgatgat gcgctggcgc tccgcccgca
    16201 gcgtgagcgc ctgggcctgg aagtcgagcg aatcggtcat gcggggcatt gcggtgtcct
    16261 cagggccgtc ggcagcgtgg atgcggggtt ccgggcacgg tacgacggca catggaacgg
    16321 gattgtggaa gtcaaggcgc ggaactgaag gccgataaag cgggggaagg gcctgcattt
    16381 cgcggcaccg tcgccgggcc cggaccctaa agtagccatc acccgcaatc ctttggccga
    16441 cgtccgtgac tcctgcccgc gccctctccc ccgatcgcct cgcccgccgc ctcgcggccg
    16501 ccgggacgtt cgcggccgcg ctggccgggc cggcgctggc gcaggacgag cccgccttcg
    16561 c
//
LOCUS       DASVLN010000838         2574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_529200, whole genome shotgun sequence.
ACCESSION   DASVLN010000838 DASVLN010000000
VERSION     DASVLN010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529200"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..2351
                     /locus_tag="VF453_19800"
     CDS             198..2351
                     /locus_tag="VF453_19800"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7689960.1"
                     /translation="MADNVAPVNRFARSASIAVRLALAFAMLLALLAGVVALALKQLD
                     RVGESGRIVAESSLRQVLLARQAEKAAQAGAQQLHTLFLLDTRQDRVPVYAMIDTEFG
                     EQKIALSQLLADAHAVEDAVVVERLIATRGRFEAAFQHTVNEVELDMNAARPIMVKET
                     LPALNDMLDALDALVAVKSAQANATIASIKVQQAQSRRDVLELSGLAVVVALLCAVMI
                     TRSITRPLAQTVALAREIADGKLDSPMPEAGRDEVGGLIKAIGEMRDSLAQREARIVD
                     LAFRDPLTGLANRTLFNDRLDQAVGTATRTGHAMSVLLIDLDRFKEVNDVLGHPVGDE
                     LLNQVSGRLTRELRRTTDTVARIGGDEFAVLLPAQGREGAVLLARQLLAALDMPVTIE
                     GQTVDVSGSIGIATFPEDGATTTELMAHADSAMYVAKQASSGYAAFDQRMARTGEHGL
                     SLLSDLRRAVDENQFYLQFQPKVSLADRSCLSAEVLIRWRHPTRGFVPPDQFIPFAER
                     TGAIKSITRWVLARGLAQLAQWRAQGLEMSLNINVSTRDLVGQDLPALVKAQLLAEGV
                     AARHLCLEVTESAIMEDPAHALASLQALHDMGVRLSIDDFGTGYSSLAYLKKLPVQEL
                     KIDRSFVMNLDRDDDDINIVRSTIDMAHHMGLQVTAEGVETESVAGKLRSLGCDDAQG
                     YLFSRPLNADDFVAWAIVAQAQRPRAAAAAKPVAG"
BASE COUNT          376 a          951 c          893 g          354 t
ORIGIN      
        1 tggcgagggg aaggtcgagc tgggcgtcca tcctcgaggt atcggcgcgc cgcgcgtgcc
       61 actgaaggcg gcgcggcgtg acaagcgtct cgctcggaca gcccgcggtg cgcgaggacg
      121 cgctcaattt ccgcgtcgtg ccggtcgata accgtcagac accgccgtcc ctgaccgccc
      181 cgccgccttc tcgtcccatg gccgacaacg tcgcccccgt caaccgcttc gcccgcagcg
      241 cgtcgatcgc cgtgcgcctc gcgctggcct tcgcgatgct gctcgccctg ctggcgggcg
      301 tcgtcgcgct cgcgctcaag cagctggacc gggtgggcga gtcgggccgc atcgtcgccg
      361 aatccagcct gcgccaggtg ctgctcgcgc gccaggcgga gaaggccgcc caggccggcg
      421 cgcagcagct gcacacgctg ttcctgctgg acacgcgcca ggaccgcgtc cccgtctacg
      481 cgatgatcga caccgagttc ggcgagcaga agatcgcgct gtcgcagctg ctggccgacg
      541 cccacgccgt cgaggacgcc gtcgtcgtcg agcgcctgat cgccacccgc ggccgcttcg
      601 aggccgcgtt ccagcacacc gtcaacgagg tggagctgga catgaacgcg gcgcgcccga
      661 tcatggtcaa ggagacgctg ccggcgctca acgacatgct cgacgcgctg gacgccctgg
      721 tcgccgtgaa gtcggcgcag gccaacgcca cgatcgccag catcaaggtg cagcaggcgc
      781 agagccggcg cgacgtgctc gagctgtcgg gtctcgcggt ggtcgtcgcg ctgctgtgcg
      841 cggtgatgat cacgcgctcg atcacgcgcc cgctcgcgca gacggtggcc ctcgcgcgcg
      901 agatcgccga cggcaagctc gattcgccga tgcccgaggc cggccgcgac gaggtcggcg
      961 ggctgatcaa ggcgatcggc gagatgcgcg acagcctcgc gcagcgcgag gcgcgcatcg
     1021 tcgacctcgc gttccgcgat ccgctcacgg gcctcgccaa ccgcacgctg ttcaacgacc
     1081 gcctcgacca ggccgtgggc accgccaccc gcaccgggca tgccatgtcg gtgctgctga
     1141 tcgacctgga tcgcttcaag gaagtcaacg acgtcctcgg gcatccggtc ggcgacgagc
     1201 tgctgaacca ggtctccggc cgcctcacgc gcgagctgcg ccgcaccacc gacaccgtgg
     1261 cgcgcatcgg cggcgacgag ttcgcggtgc tgctgcccgc ccagggccgc gagggcgcgg
     1321 tgctgctggc gcgccagctg ctcgccgcac tcgacatgcc ggtcacgatc gagggccaga
     1381 cggtggacgt gagcggctcg atcggcatcg ccaccttccc cgaggacggc gcgacgacca
     1441 ccgagctgat ggcgcatgcc gactcggcga tgtacgtcgc caagcaggcc tccagcggct
     1501 acgcggcctt cgaccagcgc atggcgcgca ccggcgagca tggcctgagc ctgctgtccg
     1561 acctgcgccg cgcggtcgac gagaaccagt tctacctgca gttccagccc aaggtctcgc
     1621 tcgcggaccg cagctgcctg tcggccgagg tgctgatccg ctggcgccac ccgacgcgcg
     1681 gcttcgtgcc gcccgaccag ttcatcccgt tcgccgagcg caccggcgcg atcaagtcga
     1741 tcacgcgctg ggtgctggcg cgcggcctgg cgcagctcgc gcagtggcgg gcgcaggggc
     1801 tggagatgtc gctgaacatc aacgtctcga cgcgcgacct cgtcgggcag gaccttccgg
     1861 cgctcgtgaa ggcgcagctg ctggccgagg gcgtcgccgc gcgccacctc tgcctggagg
     1921 tgaccgagag cgcgatcatg gaggaccccg cgcacgcgct cgcgtcgctg caggcgctgc
     1981 acgacatggg cgtgcggctg tcgatcgacg acttcggcac cggctactcc tcgctcgcgt
     2041 acctgaagaa gctgccggtg caggagctca agatcgaccg ctcgttcgtg atgaacctcg
     2101 accgcgacga cgacgacatc aacatcgtgc gctcgacgat cgacatggcg caccacatgg
     2161 gcctgcaggt gacggccgag ggcgtcgaga cggagtcggt cgccggcaag ctcaggagcc
     2221 tgggctgcga cgacgcgcag ggctacctct tcagccggcc gttgaacgcc gacgacttcg
     2281 tcgcctgggc gatcgtcgcg caagcccagc gcccgcgcgc cgccgcggcc gcgaagcccg
     2341 tcgccggctg aggcgcagca gcctccggtt cgccgccagc gggcggacac cgcgtccgcg
     2401 ccccgccggc cgtgctcgac agtgggcatg gcccatcgac ccgctcggac gctgcattga
     2461 catgtccccg gtcgaggtgc aattctgcgg cctcgatcca gagggaagcg accgcagatg
     2521 acgctggggc cagacggtcc gcgcgccccg ggtccgccgg acgacgcctc ggcc
//
LOCUS       DASVLN010000839         7263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_204700, whole genome shotgun sequence.
ACCESSION   DASVLN010000839 DASVLN010000000
VERSION     DASVLN010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204700"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..2862)
                     /gene="polA"
                     /locus_tag="VF453_19805"
     CDS             complement(88..2862)
                     /gene="polA"
                     /locus_tag="VF453_19805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX7689961.1"
                     /translation="MTDTTLLLVDGSSYLYRAYHAMPDLRGPEGVPTGAIHGMVAMMK
                     RLREQIGADYAACVFDAPGKTFRDEWYPEYKANRSSMPEDLSRQIEPIHEVVRLLGWP
                     IVMVPGIEADDAIGTLARAASEAGWKTVISTGDKDLAQLVDENVTLINTMTNETLDVA
                     GVLAKFGVPPHQIIDYLSLVGDTVDNVPGVNKVGPKTAVKWLLEHGSLDGVIAAAPTM
                     KGAVGEHLRAALDWLPQGKRLVTVVRDSDLSGHVAGWPVLDALALKPVDHDQLLEFYK
                     RFGFRSWRQELEKQLGLEGEATPAATLAPATAAAPAEGEPVAPPIELAHEYEAIYTFE
                     RLDAWIERLRAAELSALDTETDSLDAMVARIVGISFAVEPGRAAYVPLRHDYPGAPDQ
                     LPFDEVIARLKPWLEDANAPKVGQHVKYDTHVFANIGVAVRGWQHDTLLQSYVLEAHR
                     QHGLASLAERHLGRKGLDYEDLCGKGAKQIPFSQVDIERATRYSGEDSEMTLHVHQVL
                     WPRLAANAGMRYVYEQIEMPVSEVLARVERHGVLIDARCLAEQSRQLAERMMQLEQEA
                     YDIAGQPFSLGSPKQIGEILFGRLGLPVVKKTASGAPSTDEEVLEKLALDYPLPARIL
                     EHRGLAKLKGTYTDKLPQMVNPATGRVHTHYAQAVAVTGRLSSNDPNLQNIPIRTPEG
                     RRVREAFVAPPGHVVVSADYSQIELRLMAHISQDPGLLKAFTEGMDVHRATAAEVFNL
                     AAEEITSEQRRYAKTINFGLIYGMGAFGLASSLGIEQKAARDYIDRYFARFAGVKRYM
                     DETKASAAATGYVETLFGRRIELPEIRGGNGPRRSAAERQAINAPMQGTAADLIKLAM
                     LAVQKALDDEGRATRMVMQVHDELVFEVPEAEVDWARSEIPRLMAGVARLSVPLIAEV
                     GVGANWDEAH"
     gene            2974..3369
                     /locus_tag="VF453_19810"
     CDS             2974..3369
                     /locus_tag="VF453_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689962.1"
                     /translation="MTARTASTPVLAAILALAAACAHAAPAEPAPAAKPAPQAQPQDA
                     QPVVDPSAPDLVQPAPPSKVKEPKVEHLVTEDSQVRIEETRVRGVTKRIVVHSKIPGM
                     GSYEIQPRDPGVDPENDKGAGMRLFQFGF"
     gene            3457..4440
                     /locus_tag="VF453_19815"
     CDS             3457..4440
                     /locus_tag="VF453_19815"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX7689963.1"
                     /translation="MAVYTEVDAVAAQALLSRCDAGSLLEMRGCATGIENTNYFVVSD
                     KGAWVLTVFERLGFAQLPFYLGLMQHLARKGLPVPEPRTDASGNFLHEIAGKPAALVD
                     MLPGASIEAPMLAHCSLLGVTLAHLHQAALDYPVRQPNLRGLDWWERTVPVVKPHVDP
                     ALAARLDEELAFQQRFHAAESTRALPAGPVHADLFRDNALFDGPPDAPRLSGVFDFYF
                     AGVDHHAYDLAVCINDWCVDAGTGRLEPEHAQALLRAYHLHRPMSAAELRALPAMRRA
                     AALRFWLSRLADWYLPREAALLKPKDPTHFERVLLDCIEDPWHPELDTLLA"
     gene            4457..5359
                     /locus_tag="VF453_19820"
     CDS             4457..5359
                     /locus_tag="VF453_19820"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HEX7689964.1"
                     /translation="MTPDATTARVSVRIREVPAAAGLRWIRLGWRAFARQPAGFLGMF
                     ALFLLLGFLWSLPLGVVLVPLGNALGLGTAPAEFLSFLPMPLLTLGFMAATEAVTNDL
                     RIRPAMMFDALRGPRARPRSLLAVGVGYVVLAALAWFVGDSLDHGEAAAWLAQPITQP
                     AQAGDPAAIKPLSDSAQLALLLKAAIVALGSVPLWHAPALVAWARQGAAKAMFASVLG
                     LWRSRGALALHLLGWLAIVLVFSMACGVLMAVLQGSILVIAVMFVGGWVMTAWFYATL
                     WFGFQDTFDIVDLGPDAPRRALQR"
     gene            5511..6641
                     /locus_tag="VF453_19825"
     CDS             5511..6641
                     /locus_tag="VF453_19825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX7689965.1"
                     /translation="MKTIDRLNRLATSHGAIEPGHGLTTGVLALVLAVLCFLGVMAFH
                     FPQYLTTPQLRQSYSVETMRTVLYWAMVVAGGMSIWNILFARARWLAAAAFALVALSA
                     LLGGSKVPVHDFPDHTPYIGLDWFVLDLLGSSLIFVFIEKLFALRREQPVFRAEWQTD
                     LHHFIVNHMIVGFVLLATNLMVHRLFGWAAKDGVQAWVRDLPFPVALFLIILVADLVQ
                     YWTHRAYHEIPLLWRLHAVHHSVKSMDWLAGSRQHVLELLITRTLVLAPIFVLGFDKG
                     VIDAYIVIVGFQAVFNHANVSVRLGPLRYVVVTPNFHHWHHSQDDAAIDRNYAAHLAF
                     LDYLFGTAVKSDEPWPQQYGVKGDYMPDGFWRQLLFPFTWKG"
     gene            complement(6667..>7263)
                     /locus_tag="VF453_19830"
     CDS             complement(6667..>7263)
                     /locus_tag="VF453_19830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7689966.1"
                     /translation="VVLAPAGGGEAVAPATGYAIEALEGAGFHEFLRYLEDQLSDNGR
                     DGLYFAPLPRAESHVPPELVESLLEGRRVDVGAQGWRRAWVLRDRGAGGAIAGHVDLR
                     GHPSRHSGHRALLGMGVHRDHRRRGLARRLLAHAQGWAAQQPGLAWIDLTVLSTNEPA
                     VALYRAADFQVNGGLADRFVLDGRSVGELFMSRRIPRA"
BASE COUNT          915 a         2664 c         2553 g         1131 t
ORIGIN      
        1 ggcaggcccg gcccggtcac ggcgcgactc cggccggcac gcgccgcgcg ggggatgccg
       61 cccctggccg cgtcctgcgt gcggcgttca atgcgcctcg tcccagttcg cgccgacgcc
      121 cacctcggcg atcagcggga cgctcaggcg cgcgacgccc gccatcaggc gcgggatctc
      181 ggagcgcgcc cagtcgacct cggcctccgg cacctcgaag acgagctcgt cgtgcacctg
      241 catcaccatg cgcgtggcgc ggccctcgtc gtcgagcgcc ttctgcaccg ccagcatcgc
      301 cagcttgatg aggtcggccg cggtgccctg catcggcgcg ttgatggcct ggcgctcggc
      361 ggccgagcgc cgcgggccgt tgccgccgcg gatctcgggc agctcgatgc ggcgaccgaa
      421 cagcgtctcg acgtagccgg tcgccgcggc gctggccttg gtctcgtcca tgtagcgctt
      481 gacgccggcg aagcgcgcga agtagcggtc gatgtagtct cgggccgcct tctgctcgat
      541 gcccagcgag gacgccagcc cgaacgcgcc catgccgtag atcaggccga agttgatcgt
      601 cttggcgtag cggcgctgtt cgctggtgat ctcctcggcc gccaggttga agacctcggc
      661 ggccgtcgcg cggtgcacgt ccatgccctc ggtgaaggcc ttgagcagcc cgggatcctg
      721 gctgatgtgg gccatcagcc gcagctcgat ctgcgagtag tccgccgaca ccaccacgtg
      781 ccccggcggc gcgacgaagg cctcgcgcac gcgccggccc tcgggtgttc ggatcgggat
      841 gttctgcagg ttggggtcgt tgctggacag gcgccccgtc accgccaccg cctgcgcgta
      901 gtgcgtgtgc acccggccgg tcgccgggtt gaccatctgc ggcagcttgt cggtgtacgt
      961 gcccttgagc ttggccaggc cgcggtgctc gaggatcctt gccggcagcg gatagtcgag
     1021 cgcgagcttc tcgagcacct cctcgtcggt cgacggcgcc ccgctggcgg tcttcttgac
     1081 caccggcagg cccaggcgcc cgaacaggat ctcgccgatc tgcttgggcg agccgaggct
     1141 gaacggctgg ccggcgatgt cgtaggcctc ctgctccagc tgcatcatgc gctcggcgag
     1201 ctggcgcgac tgttcggcca ggcagcgggc gtcgatcagc acgccgtggc gctcgacgcg
     1261 cgccagcacc tcgctgaccg gcatctcgat ctgctcgtag acgtagcgca tgcccgcgtt
     1321 cgccgccagg cgcggccaca gcacctggtg gacgtgcagc gtcatctcgc tgtcctcgcc
     1381 cgagtagcgc gtcgcgcgtt cgatgtcgac ctgcgagaac gggatctgct tcgcgccctt
     1441 gccgcacagg tcctcgtagt cgaggccctt gcggcccagg tggcgctcgg cgaggctcgc
     1501 gaggccgtgc tgacggtgcg cctcgaggac gtagctctgg agcagcgtgt cgtgctgcca
     1561 gccgcgcacc gcgacgccga tgttggcgaa gacgtgggtg tcgtacttga cgtgctggcc
     1621 gaccttgggc gcgttcgcat cctcgagcca tggcttgagg cgggcgatga cctcgtcgaa
     1681 gggcagctgg tcgggcgccc ccggatagtc gtggcgcagc gggacgtagg ccgcgcggcc
     1741 cggctcgacc gcgaacgaga tgccgacgat gcgggcgacc atggcgtcca gggagtccgt
     1801 ctcggtgtcg agcgccgaca gctcggcggc acgcaggcgc tcgatccagg cgtccaggcg
     1861 ctcgaaggtg tagatggctt cgtactcgtg cgcgagctcg atgggcgggg cgaccggctc
     1921 gccctcggcc ggcgcggcgg cggtcgcggg cgccagcgtc gccgcgggcg tcgcctcgcc
     1981 ttcgaggccc agctgcttct cgagctcctg gcgccagctg cggaagccga agcgcttgta
     2041 gaactcgagc agctggtcgt ggtcgaccgg cttgagcgcg agcgcgtcca gcaccggcca
     2101 gccggcgaca tggccgctca ggtcgctgtc gcgcaccacg gtgacgaggc gcttgccctg
     2161 cggcagccag tccagagcgg cccgcaggtg ctcgccgacg gcgcccttca tcgtcggcgc
     2221 cgccgcgatc acgccgtcga gcgaaccgtg ctccagcagc cacttcaccg cggtcttggg
     2281 gccgaccttg ttgacgccgg gcacgttgtc gacggtgtcg ccgacgagcg agaggtagtc
     2341 gatgatctgg tgcggcggca ccccgaactt ggcgagcacg ccggccacgt ccagcgtctc
     2401 gttggtcatc gtgttgatca gggtgacgtt ctcgtcgacc agctgcgcga ggtccttgtc
     2461 gccggtggag atcacggtct tccagcccgc ctcgctggcc gcgcgggcga gcgtgccgat
     2521 cgcgtcgtcg gcctcgatgc ccggcaccat gacgatcggc cagccgagca ggcgcacgac
     2581 ctcgtggatc ggctcgatct ggcgcgacag gtcctcgggc atggacgagc ggttcgcctt
     2641 gtactcggga taccactcgt cgcggaaggt cttgccgggc gcatcgaaga cgcaggcggc
     2701 gtagtccgcg ccgatctgct cgcgcaggcg cttcatcatc gcgaccatgc cgtggatggc
     2761 gccggtcggc acgccctcgg gcccgcgcag gtcgggcatg gcgtggtagg cgcggtagag
     2821 gtagctggag ccgtccacca gcagcagggt cgtgtcggtc atggggcgca ttgtgcccag
     2881 gatgcgaggg cgccgcggag cgttcgcgcg ggcgaaaccg gcggttccgc cttcgcgtcc
     2941 tccgccgcga cacgaacgtc ttagaatccg cccatgaccg cccgcaccgc ttcgacccct
     3001 gtcctcgccg cgatcctcgc gctcgccgcc gcctgcgcgc acgcggcccc ggccgagccc
     3061 gctccggcgg ccaagccggc cccgcaggcg cagccgcagg acgcgcagcc cgtggtcgac
     3121 ccgagcgcgc cggacctggt gcagccggcg ccgcccagca aggtcaagga gcccaaggtc
     3181 gagcacctcg tgaccgagga ctcgcaggtg cgcatcgagg agacccgcgt gcgcggcgtg
     3241 accaagcgga tcgtcgtgca ctcgaagatc cccggcatgg gcagctacga gatccagccg
     3301 cgcgatccgg gcgtcgaccc ggagaacgac aagggcgcgg gcatgcgcct gttccagttc
     3361 ggtttctgac gccgtgaccc gccgcccggt cgccgcggtc gccccgcagc gcgcgcagtc
     3421 gcgcacccga cgccgtcccg cacgaggctg acgcgcatgg ccgtctacac cgaagtcgac
     3481 gccgtcgcgg cgcaggcgct gctgtcgcgc tgcgatgccg gcagcctgct ggagatgcgc
     3541 ggctgcgcga ccggcatcga gaacaccaac tacttcgtcg tctcggacaa gggcgcgtgg
     3601 gtgctgacgg tgttcgagcg cctcggcttc gcccagctgc cgttctacct cggcctcatg
     3661 cagcacctcg cgcgcaaggg cctgccggta cccgagccgc gcaccgacgc ctccggcaac
     3721 ttcctgcacg agatcgccgg caagccggcg gcgctcgtgg acatgctgcc cggtgcgagc
     3781 atcgaggcgc cgatgctcgc gcactgctcg ctgctgggcg tgaccctcgc acacctgcac
     3841 caggccgcgc tggactaccc ggtgcgccag cccaacctgc gcggcctcga ctggtgggaa
     3901 aggaccgtcc ccgtcgtcaa gccgcatgtg gatccggcgc tcgcggcacg cctggacgag
     3961 gagctcgcct tccagcagcg cttccacgcc gccgagtcga cgcgcgcgct gcccgccggc
     4021 ccggtgcatg ccgacctgtt ccgcgacaac gcgctgttcg acggcccacc cgacgcgccc
     4081 cgcctgtcgg gcgtgttcga cttctacttc gcgggcgtcg accaccacgc ctacgatctc
     4141 gcggtctgca tcaacgactg gtgcgtcgac gcgggcacgg gccgcctgga gcccgagcac
     4201 gcccaggccc tgctgcgcgc ctatcacctg caccgcccga tgagcgccgc cgagctgcgc
     4261 gccctgcccg cgatgcggcg cgccgcggcg ctgcgcttct ggctctcgcg cctggccgac
     4321 tggtacctgc ctcgcgaggc ggcgctgctc aagcccaagg atccgacgca cttcgagcgc
     4381 gtgctgctcg actgcatcga ggatccgtgg catcccgagc tcgataccct gctcgcgtga
     4441 agggcccatc tccagcatga cccccgacgc gacgacggcg cgcgtgtccg tgcgcatccg
     4501 cgaggtgccc gcggccgccg gactgcggtg gatccgcctc ggctggcgcg cgttcgcgcg
     4561 gcagccggcg ggcttcctcg ggatgttcgc gctgttcctg ctgctcggct tcctgtggtc
     4621 gctgccgctg ggcgtggtgc tggtgcccct gggcaacgcg ctcgggctgg gcacggcgcc
     4681 ggcggagttc ctgagcttcc tgccgatgcc gctgctgacg ctcgggttca tggccgccac
     4741 cgaagcggtg accaacgacc tgcgcatccg gccggccatg atgttcgacg cgctgcgcgg
     4801 tccgcgcgcg cggccgcgct cgctgctggc ggtcggcgtg ggctacgtcg tgctggccgc
     4861 gctcgcctgg ttcgtgggcg acagcctcga ccacggcgag gccgccgcct ggctcgcgca
     4921 gccgatcacg cagcccgcgc aggcgggcga tccggcggcc atcaagccgt tgagcgactc
     4981 ggcacagctc gcgctgctgc tgaaggcggc catcgtggcg ctcgggtcgg tgccgctgtg
     5041 gcacgcgccg gcgctggtcg cgtgggcgcg ccagggcgcg gccaaggcga tgttcgcgag
     5101 cgtgctcggc ttgtggcgca gccgcggcgc gctggcgctg cacctgctcg gatggctggc
     5161 catcgtgctg gtgttctcga tggcctgcgg cgtgctgatg gcggtgctgc agggctcgat
     5221 cctcgtcatc gccgtcatgt tcgtgggcgg ctgggtgatg acggcgtggt tctacgccac
     5281 gctctggttc ggcttccagg acaccttcga catcgtcgac ctgggccccg acgcgccgcg
     5341 ccgcgcgctg cagcgctgac ggcggcggcg ccgccgccca ttccgtcacg ccggcggacc
     5401 gcctccccct ccttcgggcg aatcgcgccg cgccgctttc tgcttcaatg cggccttcgg
     5461 gccgcccggc gggcggcgcc gcgacgacac acccgcagga gggacaccgc atgaagacga
     5521 tcgacaggct caacaggctc gcgacctcgc acggcgccat cgagccgggc catggcctga
     5581 ccaccggcgt gctggcgctc gtcctcgccg tgctgtgctt cctcggcgtg atggccttcc
     5641 acttcccgca gtacctgacg acgccgcagc tgcgccagtc ctacagcgtc gagacgatgc
     5701 gcacggtgct gtactgggcg atggtggtgg ccggcgggat gtcgatctgg aacatcctgt
     5761 tcgcacgcgc ccgctggctc gcggccgcgg cgttcgcgct ggtggcgctg tcggcgctgc
     5821 tgggcggctc caaggtgccg gtgcacgact tccccgacca cacgccctac atcggcctcg
     5881 actggttcgt gctcgacctg ctcggctcgt cgctgatctt cgtcttcatc gagaagctgt
     5941 tcgcgctgcg ccgcgagcag ccggtcttcc gcgccgagtg gcagaccgac ctgcaccatt
     6001 tcatcgtcaa ccacatgatc gtcggcttcg tgctgctggc gaccaacctg atggtgcacc
     6061 ggttgttcgg ctgggccgcc aaggacggcg tgcaagcctg ggtgcgcgac ctgccgttcc
     6121 cggtcgccct gttcctgatc atcctcgtcg ccgacctcgt gcagtactgg acgcaccgcg
     6181 cgtaccacga gatcccgctg ctttggcgcc tgcacgcggt gcaccactcg gtcaagagca
     6241 tggactggct cgccggctcg cgccagcacg tgctggagct gctgatcacg cgcacgctcg
     6301 tcctcgcgcc gatcttcgtg ctgggcttcg acaagggtgt gatcgacgcc tacatcgtga
     6361 tcgtcggctt ccaggcggtc ttcaaccacg ccaacgtcag cgtgcggctg gggccgctgc
     6421 gctacgtcgt cgtcacgccc aacttccacc actggcacca ctcgcaggac gacgcggcga
     6481 tcgaccgcaa ctacgcggcg cacctggcct tcctcgacta cctgttcggc accgcggtga
     6541 agtccgacga gccgtggccg cagcagtacg gcgtgaaggg cgactacatg cccgacgggt
     6601 tctggcgcca gctgctgttc ccgttcacct ggaagggctg agaggccggg gacgtccgcc
     6661 cccgcgtcac gcgcgcggga tgcgccggct catgaacagc tcgccgaccg atcggccgtc
     6721 cagcacgaag cggtcggcca ggccgccgtt gacctggaag tcggccgcgc ggtagagcgc
     6781 gaccgcgggc tcgttggtcg agagcacggt caggtcgatc caggcgagcc ccggctgctg
     6841 cgccgcccag ccctgcgcgt gcgcgagcag gcgccgggcg aggccgcgcc ggcgatggtc
     6901 gcggtgcacg cccatgccca gcagcgcgcg atgaccgctg tggcggctcg ggtggccgcg
     6961 caggtcgacg tgcccggcga tcgccccgcc cgcgccacgg tcgcgcagca cccaggcgcg
     7021 gcgccagccc tgcgcgccga cgtccacccg ccggccctcg agcaacgact cgacgagctc
     7081 gggcggcaca tgcgactccg cgcgcggcag cggcgcgaag tacaggccgt cgcggccgtt
     7141 gtcggacagc tggtcctcga gatagcgcag gaactcgtgg aagccggcgc cctcgagcgc
     7201 ctcgatcgcg tagccggtgg ccggcgccac cgcctcgccg ccgcccgccg gcgcgagcac
     7261 cac
//
LOCUS       DASVLN010000840         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_98273, whole genome shotgun sequence.
ACCESSION   DASVLN010000840 DASVLN010000000
VERSION     DASVLN010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98273"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1475
                     /locus_tag="VF453_19835"
     CDS             21..1475
                     /locus_tag="VF453_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7689967.1"
                     /translation="MSQAALVEALRGLLPPHALLWRAEDTIPYECDGLTAYRERPAAV
                     ALPETQEHVAAVLATCHRLGVPVVARGAGTGLSGGAMPHAQGVTLSLAKFNRIVKVDV
                     RARTATVQSGVRNAAISEAAAPHGFYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLT
                     LHNVLQVRGFLADGSPATFGSLALDAPGLDLLPLVVGSEGMLAVVTEVVVRLTPKPQV
                     ARCIMASFADVRHAGEAVAAIIADGLIPAGLEMMDKPMTAAVEAFVHAGYDLDAAAIL
                     LCESDGTPEEVDEEIARMEAVLRAAGATRLQVSDSEAQRLKFWSGRKNAFPASGRISP
                     DYMCMDSTIPRARLADMLLAIAEMERKYGLRCCNVFHAGDGNLHPLILFDANAPDELH
                     RAEAFGAEILELSVAMGGTISGEHGVGAEKLNSMCVQFSPAERAQMEAVKRAFDPRGL
                     LNPGKVIPSLHRCAEYGRMHVRRGVLAFPELERF"
     gene            1477..>2127
                     /gene="glcE"
                     /locus_tag="VF453_19840"
     CDS             1477..>2127
                     /gene="glcE"
                     /locus_tag="VF453_19840"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEX7689968.1"
                     /translation="MDADSLIFLAERVREASETRIPLRIRGGDTKAFLAGLADGPVLD
                     MRVHAGIVDYAPSELVVTVRAGTPLVELERALADRGQCLPFDPPRFADERGLLAGTVG
                     GMVAAGLAGPGRASVGGVRDYVLGALLLDGQGELLHFGGQVMKNVAGYDVSRVLAGSW
                     GALGAICEVSLKVLPTPPATLTLRFDLPQDAALERLHAWAGQPLPLNASVWWRDTLA"
BASE COUNT          272 a          790 c          777 g          288 t
ORIGIN      
        1 aggggccgga ggcgccgccg gtgtctcagg ccgcgctggt cgaggccctg cgcggcctgc
       61 tgccgcccca cgcgctgctc tggcgcgccg aggacacgat cccctacgaa tgcgacggcc
      121 tgaccgccta ccgcgagcgc cccgccgccg tcgcgctgcc cgagacgcag gagcatgtcg
      181 ccgccgtcct ggccacctgc catcgcctgg gcgtgccggt ggtcgcgcgc ggcgcgggca
      241 ccgggctgtc cggcggcgcg atgccgcacg cgcagggcgt gacgctgtcg ctggccaagt
      301 tcaaccgcat cgtgaaggtc gacgtccgcg cccgcaccgc caccgtgcag tccggcgtgc
      361 gcaatgcggc gatcagcgag gccgccgcac cccacggctt ctattacgcg cccgacccgt
      421 cgagccagat cgcctgcacg atcggcggca acgtcgccga gaactcgggg ggcgtgcact
      481 gcctgaagta cggcctgacg ctgcacaacg tgctgcaggt gcgcggcttc ctggccgacg
      541 gcagcccggc caccttcggt tcgctggcgc tcgacgcgcc cggcctcgac ctgctgccgc
      601 tggtcgtcgg cagcgagggc atgctggcgg tggtcacgga ggtcgtcgtc aggctcacgc
      661 ccaagccgca ggtcgcgcgc tgcatcatgg ccagcttcgc cgacgtgcgc catgccggcg
      721 aggcggtcgc ggcgatcatc gcggacggcc tgatcccggc cggcctggag atgatggaca
      781 agccgatgac cgccgcggtc gaggccttcg tgcacgccgg ctacgacctc gacgccgccg
      841 ccatcctgct gtgcgagagc gacggcacgc ccgaggaagt cgacgaggag atcgcccgca
      901 tggaggccgt gctgcgcgcc gccggcgcga cgcgcctgca ggtcagcgac agcgaggcgc
      961 agcgcctgaa gttctggagc gggcgcaaga acgccttccc cgcgtcgggc cgcatcagcc
     1021 ccgactacat gtgcatggac tcgacgatcc cgcgcgcgcg gctggccgac atgctgctgg
     1081 cgatcgcgga gatggagcgc aagtacggcc tgcgctgctg caacgtcttc cacgccgggg
     1141 acggcaacct tcatccgctg atcctgttcg acgccaacgc ccccgacgag ctgcaccgcg
     1201 ccgaggcctt cggcgccgag atcctcgagc tctcggtcgc gatgggcggc acgatcagcg
     1261 gcgagcacgg cgtcggcgcc gagaagctga actcgatgtg cgtgcagttc tcgcccgccg
     1321 agcgcgcgca gatggaggcg gtcaagcgcg ccttcgaccc gcgcggcctg ctcaacccgg
     1381 gcaaggtgat cccgtcgctg caccgctgcg ccgagtacgg ccgcatgcac gtgcgccgcg
     1441 gcgtcctcgc cttccccgag ctggagcggt tctgagttgg acgccgacag cctgatcttc
     1501 ctggccgagc gcgtgcgcga ggcctccgag acccgcatcc cgctgcgcat ccgcggcggc
     1561 gacaccaagg ccttcctcgc cgggctggcc gacgggccgg tgctggacat gcgcgtgcac
     1621 gcgggcatcg tcgactacgc gccgagcgag ctcgtcgtca ccgtgcgcgc cggcacgccg
     1681 ctggtggagc tcgagcgcgc gctcgccgat cgcgggcagt gcctgccgtt cgacccgccg
     1741 cgcttcgccg acgagcgcgg actgctggcc ggcacggtgg gcggcatggt ggccgcgggc
     1801 ctcgccggtc cgggccgcgc gagcgtgggc ggcgtgcgcg actacgtgct gggcgcgctg
     1861 ctgctcgacg gccagggcga gctgctgcac ttcggcggcc aggtgatgaa gaacgtcgcc
     1921 ggctacgacg tctcgcgcgt gctggcgggc tcgtggggcg cgctgggcgc gatctgcgag
     1981 gtctcgctga aggtgctgcc gacgccgccg gccacgctca cgctgcgctt cgacctgccg
     2041 caggacgcgg cgctcgagcg gctgcacgca tgggccggcc agccgctgcc gctgaacgcg
     2101 agcgtctggt ggcgcgacac cctggcc
//
LOCUS       DASVLN010000841         6749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_529350, whole genome shotgun sequence.
ACCESSION   DASVLN010000841 DASVLN010000000
VERSION     DASVLN010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529350"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..276)
                     /locus_tag="VF453_19845"
     CDS             complement(4..276)
                     /locus_tag="VF453_19845"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7689969.1"
                     /translation="MIVCVCHRVSDRDIEHAVNHGGCQSFEELQDATRCGTHCGRCLE
                     CAEETFDAAMACRPAAARVIAIAPVAPVLPATAQPMPVVQPAPLAA"
     gene            complement(496..972)
                     /gene="bfr"
                     /locus_tag="VF453_19850"
     CDS             complement(496..972)
                     /gene="bfr"
                     /locus_tag="VF453_19850"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX7689970.1"
                     /translation="MQGDPQVVAHLQAQLKNELTAINQYFVHYRMFKHWGFGRMAKKE
                     YEESIGEMKHADKLMDRIFMLDGLPNLQDLGKLMIGETLLEALGCDLKLERAAQATIK
                     DGIACCETARDYVSREILQDILDDTEEHIDFLETQLELAGKVGEQNFLQSMMGEPS"
     gene            complement(1095..1541)
                     /locus_tag="VF453_19855"
     CDS             complement(1095..1541)
                     /locus_tag="VF453_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689971.1"
                     /translation="MALNYLPFEPSPVTVRRRGGRGQRAALAIAVCWFLAGGAAHFLF
                     TDALVRVVPPAVPDAGDVVLLTGLLWLLGALGLALPWTRRAAGWSLLLLVIAVTPVHV
                     YMLQAHDRFPSLPLWLLWARLPLQGVVLWVVWWGSRWRKPPRGLLY"
     gene            complement(1728..2783)
                     /locus_tag="VF453_19860"
     CDS             complement(1728..2783)
                     /locus_tag="VF453_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7689972.1"
                     /translation="MRNHPLGRTGLFVSELCLGTMTFGGSDETGIWGHIGRLQQADAE
                     RLVGQALDAGINFIDTADVYANGASEQITGQALKNLKVPRDRVVVATKVFGETGPGAN
                     MRGASRGHILDGVKASLKRLQLDHVDLYQIHGFDPATPIEETVRALDQLVRQGHVRYV
                     GVSNWAAWQIVKALGVAERLGLSRFESLQAYYTIAGRDLEREIVPMLRSEGLGLMVWS
                     PLAGGLLSGKYRDEKSAAGSRRQSFDFPPVDRPRADRVIDAMRPVAEAHGVSVARVAL
                     AWLLHQPQVTSVIVGAKRPDQLADNIAAATLALSDAELATLAEVSALPAEYPGWMFAR
                     QGEYRARQLAESSAPRR"
     gene            3179..4495
                     /locus_tag="VF453_19865"
     CDS             3179..4495
                     /locus_tag="VF453_19865"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX7689973.1"
                     /translation="MWRPDFLFGAATASFQVEGATRADGRLPSIWDTFCATPGKVHAG
                     DTGDVACDHYHRFESDLALLAGLGFRAYRFSIAWPRVMDASGRPNPRGIDFYKRLLDA
                     LEARGLQAWATLYHWDLPQHLQDRGGWLNRDTAQRFADYAELMARTFGGRVRAWMTLN
                     EPWCSAFLGHVEGRHAPGLADARYGVEAMHHLLLGHGLAVGAIRAHDRAPVGLVTNVN
                     AVAPASDSPADRLAAERAAASANHWVLDAVLLGRYPAPLAGLWPEAVVPERPGDMATI
                     AAPLDFLGINYYFRQVVESDGAHGYREHPVPGVERTQMGWEVHPQGLRDLLLEFKARY
                     PSLPPIHITENGMASDDRVEAGRVRDAQRARYFAGHLAAVDEAMRRGVDVRGYFAWSL
                     LDNFEWSYGYEKRFGIVHVDYATQARTPKDSALAFKAFLAARERAT"
     gene            4561..5805
                     /locus_tag="VF453_19870"
     CDS             4561..5805
                     /locus_tag="VF453_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7689974.1"
                     /translation="MTPIPTFGRLATALALALAASGAAHAQANRAEVIHWWTSGGESA
                     AVHTLADAYKAAGGTWVDAAIAGGEQARATAVNRMVGGTPPTAAQFNTSQQFRDLIDQ
                     GLLNDVDVVAAKNQWDRILPAPILSQIKVKGHFYAVPVDIHMPAWFWYSKAAFAKAGI
                     TAEPRSADELFADLDKLKAAGLVPLAFGGQPWQEKITFYAWLAHVGGKDMYLKLYRDK
                     DVATVESPAFRKVLASFKHLHDYVDPGSPNRNWNDATAMVISGKAGVQIMGDWAKGEF
                     GAAHQAAGKDYGCFPGFGPKSPYLVAGDVFVFPKTTDPAQVKSQQLLATVFTAPATQV
                     AFSNKKGSIPIRTDVDVKALDPCAQIGVNIMKDPSRQLPSPEMLVTPDMEGALQDVIT
                     KFWNTNESVDDAVKAIVSTLRS"
     gene            5819..>6749
                     /locus_tag="VF453_19875"
     CDS             5819..>6749
                     /locus_tag="VF453_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7689975.1"
                     /translation="MTTTPARRRPVARHAAAWMALLPMAAAVLWVYVGSVAWSVRVSF
                     SSSRTLPIGDFVGWHQYTRLFASDRWTISLENLALFGALFVLGCLAIGFVLAVLIDQR
                     VRGEGLLRTVFLYPYAMSFVATGLVWQWMLNPELGLQATVRGWGWTGFTFDWIVRQDL
                     VMVAIAIAAIWQASGLVMALMLAGLRGIEESLWMAARIDGIPAWRTYLSIVAPQLGPS
                     FATAFVLLAVGVVKVFDVVVAMTQGGPGLASEVPAKFIIDNLFGRANIGLASAASTVL
                     LLTVLALIAPGLYLRRRGARRDEAHRPQAAEALP"
BASE COUNT          968 a         2486 c         2350 g          945 t
ORIGIN      
        1 cgttcaggcc gccaggggcg ccggctgcac gaccggcatg ggctgcgcgg tggccggcag
       61 caccggggcc accggggcga tggcgatgac gcgcgccgcg gccggacggc aggccatggc
      121 ggcgtcgaag gtctcctccg cgcactccag gcagcggccg cagtgggtgc cgcagcgggt
      181 cgcgtcctgc agttcctcga agctctggca gccgccgtgg ttgacggcgt gctcgatgtc
      241 gcggtcggag acgcggtggc agacgcagac gatcacgggc ggcctcccgc ggcggcagcg
      301 gtggggcggg cgggggcggc gaaggtcatg cgtcgagttc catgcgtgag ctctggagtg
      361 aactataacg cgaatcgttc gcatttgcaa acctccaatc gaggaacccg ccgggaaagg
      421 cgacctcgat gccgtctggc gacgccgcgc cggcagcgcc gcacgagggc cgtcccgccc
      481 tgggcccggc cggcctcagc tcggctcgcc catcatcgac tgcaggaagt tctgctcgcc
      541 gaccttgccc gcgagctcga gctgggtctc gaggaagtcg atgtgctcct cggtgtcgtc
      601 caggatatcc tgcaggatct cgcgcgagac gtagtcgcgc gccgtctcgc agcaggcgat
      661 gccgtccttg atggtcgcct gcgctgcgcg ctcgagcttc aggtcgcagc ccagcgcctc
      721 cagcagcgtc tcgccgatca tcagcttgcc gaggtcctgc aggttgggca ggccgtcgag
      781 catgaagatg cgatccatga gcttgtcggc gtgcttcatc tcgccgatcg actcctcgta
      841 ttccttcttc gccatgcgcc cgaagcccca gtgcttgaac atccggtagt ggacgaagta
      901 ctggttgatc gcggtcagct cgttcttcag ttgcgcctgc aggtgcgcga caacctgggg
      961 atcgccttgc atggatgctc ctcgttcgcc gtggtggcgc cagcgccgat cctagcgcga
     1021 acgggcggcg gcgccagccg tcgacgaccg ctcgaagcac ggcggatcgc cggacgcggc
     1081 gcctcggagc gacgtcagta gagcaggccg cgcggcggct tgcgccagcg gctgccccac
     1141 cacacgaccc acagcaccac gccctgcagc ggcagccgcg cccacagcag ccacagcggc
     1201 agcgacggga agcggtcgtg cgcctgcagc atgtacacgt gcacgggcgt gacggcgatc
     1261 accagcagca gcaggctcca gccggccgcg cgccgcgtcc agggcagcgc caggccgagg
     1321 gcgcccagca gccacagcag gccggtgagc aggacgacgt cccccgcatc gggcacggcg
     1381 ggcggcacga cgcgcacgag cgcatcggtg aagaggaagt gcgcggcgcc gcccgcgagg
     1441 aaccagcaga ccgcgatcgc cagcgccgcg cgctgcccgc gcccgccgcg gcgccgcacg
     1501 gtgaccggcg agggctcgaa cggcaggtag ttcagcgcca aggacggccc tcccggcaca
     1561 cggccgcgcg gtcgtcggga acggtggcgc cggcctcgcg ccgggcccgt cgcatcgtca
     1621 ggactctcat ccggcgcgat gatcgcccag cgcctgcgcg acgcgagctc tcgcggcgaa
     1681 agagtcgtaa cgaaagccgg acggccgagc aggcgggcga cgatccttca gcgccgcggc
     1741 gcgctcgact ccgcgagctg ccgcgcccgg tactcgccct ggcgcgcgaa catccagccg
     1801 ggatactcgg ccggcagcgc gctgacctcg gccagcgtcg cgagctcggc gtcggacagc
     1861 gcgagcgtcg ccgcggcgat gttgtcggcg agctggtcgg gacgcttggc gccgacgatc
     1921 acgctggtga cctgcggctg gtgcagcagc catgcgagcg cgacccgcgc cacggagacc
     1981 ccgtgcgcct cggcgaccgg ccgcatcgcg tcgatcacgc ggtcggcgcg cggccggtcg
     2041 acgggcggga agtcgaagct ctggcggcgg ctgccggcgg cgctcttctc gtcgcggtac
     2101 ttgccgctga gcagcccacc cgcgagcggg ctccacacca tcaggcccag gccctcgctg
     2161 cgcagcatgg gcacgatctc gcgctcgagg tcgcgacccg cgatcgtgta gtaggcctgc
     2221 agcgactcga agcgcgagag gccgagccgc tccgccaccc cgagcgcctt gacgatctgc
     2281 cacgcggccc agttggagac gccgacgtag cgcacgtgcc cctggcgcac cagctggtcg
     2341 agggcgcgca ccgtctcctc gatcggcgtc gccgggtcga agccgtggat ctggtagagg
     2401 tcgacgtgat cgagctgcag ccgcttcagg ctcgccttga cgccgtcgag gatgtgcccg
     2461 cgcgacgccc cgcgcatgtt cgcgccgggg ccggtctcgc cgaagacctt ggtcgccacg
     2521 accacgcggt cgcgcggcac cttcaggttc ttcagcgcct ggccggtgat ctgctcggac
     2581 gcgccgttcg cgtagacgtc ggcggtgtcg atgaagttga tgcccgcgtc gagcgcctgg
     2641 ccgacgagac gctcggcgtc ggcctgctgc agacggccga tgtggcccca gatgccggtc
     2701 tcgtcgctgc cgccgaaggt catggtgccg aggcacagct cggagacgaa caggccggtg
     2761 cggccgaggg gatggttgcg catggacagc tccgcgtggt ggatggcgcg cggcacgcgg
     2821 ccgcgcgaag cttcgcagga taggagcgcg ccaagacttc caccgccgcg gggtcaatcg
     2881 cggcgaggct cgcgcgccga cgagcgccct ttgcacgcac gctcggtgtt ttcccggatg
     2941 agccgctgcc cgccggcgtg cagaatcgcg ccgcatggaa ccggttccat acgttccatg
     3001 acccatgatc ccggggaggc cggcgcggag cgcgcacggc ccgccgaggc caggaggcga
     3061 cgacctccga cgaacgacaa acagccgcgc cggccccgcc cgtcgcaccg cccctttcta
     3121 cacgctgctg cccgtgaccg acgacctgcc cgccatcgcc ccgccacccg gctccgccat
     3181 gtggcggccc gacttcctct tcggcgccgc cacggcgtcg ttccaggtcg agggcgcgac
     3241 ccgcgccgac ggccgcctgc cctcgatctg ggacaccttc tgcgcgacgc ccggcaaggt
     3301 acacgccggc gacaccggcg acgtcgcctg cgaccactac caccgcttcg agtccgacct
     3361 ggcgctgctg gccggcctgg gctttcgcgc ctaccgcttc tccatcgcct ggccgcgcgt
     3421 gatggacgcc tcgggccgtc cgaacccgcg cggcatcgac ttctacaagc gcctgctcga
     3481 cgcgctggag gcgcgcggcc tccaggcctg ggcgacgctc taccactggg accttcctca
     3541 gcacctgcag gatcgcggcg gctggctcaa ccgcgacacg gcgcagcgct tcgccgacta
     3601 cgccgagctg atggcgcgca ccttcggcgg ccgcgtgcgg gcgtggatga cgctcaacga
     3661 accgtggtgc tcggccttcc tcggccacgt cgaggggcgc cacgccccgg gcttggccga
     3721 cgcgcgctac ggcgtcgagg cgatgcacca cctgctgctc ggccacggcc tggcggtggg
     3781 cgcgatccgt gcccacgacc gcgcgccggt gggcctcgtg accaacgtca acgccgtcgc
     3841 gccggccagc gactcgccgg ccgaccgcct cgccgcggag cgcgccgccg cgagcgccaa
     3901 ccactgggtg ctcgacgccg tgctgctcgg ccgctatccc gcgccactcg ccggcctgtg
     3961 gcccgaggcc gtcgtgcccg aacgcccggg cgacatggcg accatcgccg cgccgctgga
     4021 cttcctgggc atcaactact acttccgcca ggtcgtcgag agcgacggcg cgcacggcta
     4081 ccgcgagcat cccgtgcccg gcgtcgagcg cacgcagatg ggctgggagg tccacccgca
     4141 gggcctgcgc gacctgctgc tcgagttcaa ggcgcgctac ccgtcgctgc cgcccatcca
     4201 catcacggag aacggcatgg ccagcgacga ccgcgtcgag gccggccgcg tgcgcgacgc
     4261 gcagcgcgcg cgctacttcg cgggccatct cgccgccgtc gacgaggcga tgcgccgcgg
     4321 cgtcgacgtc cggggctact tcgcgtggtc gctgctggac aacttcgagt ggagctacgg
     4381 ctacgagaag cgcttcggca tcgtccacgt cgactacgcg acgcaggcgc gcacgcccaa
     4441 ggacagcgca ctcgcgttca aggccttcct cgcggcgcgc gagcgcgcga cctgacgttt
     4501 tccccaagcc gccgcgaccg gcccgcgcgt cgcgtacaaa atcgacccag gagacttccc
     4561 atgaccccga tcccgacctt cggacgcctc gcgaccgcgc tcgcgctggc cctcgccgcg
     4621 agcggcgccg cgcacgccca ggccaaccgc gccgaggtga tccactggtg gacctcgggc
     4681 ggcgaatcgg ccgccgtgca cacgctggcc gatgcgtaca aggccgcggg cggcacctgg
     4741 gtggacgcgg ccatcgctgg cggcgagcag gcgcgcgcca ccgcggtcaa ccgcatggtg
     4801 ggcggcacgc cgccgacggc cgcgcagttc aacacctcgc agcagttccg cgacctgatc
     4861 gaccaggggc tgctcaacga cgtcgacgtc gtcgcggcga agaaccagtg ggacaggatc
     4921 ctgcccgcgc ccatcctgag ccagatcaag gtcaagggcc acttctacgc ggtgccggtc
     4981 gacatccaca tgcccgcatg gttctggtac tccaaggcgg cgttcgcgaa ggccgggatc
     5041 acggccgagc ccaggagcgc cgacgagctc ttcgccgacc tcgacaaact caaggcggcc
     5101 ggcctcgtgc cgctggcctt cggcggccag ccgtggcagg agaagatcac cttctacgcc
     5161 tggctcgcgc acgtcggcgg caaggacatg tacctgaagc tgtaccgcga caaggacgtc
     5221 gcgaccgtcg agtcgcccgc gttccgcaag gtgctcgcct cgttcaagca tctgcacgac
     5281 tacgtcgacc cgggctcgcc caaccgcaac tggaacgacg ccaccgcgat ggtgatctcg
     5341 ggcaaggccg gcgtgcagat catgggcgac tgggccaagg gcgagttcgg cgccgcgcac
     5401 caggccgcgg gcaaggacta cggctgcttc ccgggcttcg gcccgaagtc gccctacctc
     5461 gtcgcgggcg acgtgttcgt gttcccgaag acgaccgacc ccgcgcaggt caagtcccaa
     5521 cagctgctcg ccaccgtgtt cacggcaccg gcgacgcagg tcgccttcag caacaagaag
     5581 ggatcgatcc cgatccgcac cgacgtcgac gtcaaggcgc tggacccgtg cgcgcagatc
     5641 ggcgtcaaca tcatgaagga tccgtcgcgc cagctgccca gcccggagat gctggtcacc
     5701 cccgacatgg aaggcgcgct gcaggacgtc atcacgaagt tctggaacac caacgagtcg
     5761 gtggacgacg cggtcaaggc gatcgtctcg accctccggt cgtgaagccc gccgcgcgat
     5821 gacgacgacg cccgcacgcc gccgccccgt ggcgcgccat gcggccgcct ggatggcgct
     5881 gctgccgatg gcggcggcgg tgctgtgggt ctacgtcggc agcgtggcat ggtcggtgcg
     5941 cgtgtccttc agcagctcgc gcacgctgcc gatcggcgac ttcgtcggct ggcaccagta
     6001 cacccgcctg ttcgcgagcg accgctggac gatctcgctc gagaacctcg ccctcttcgg
     6061 ggcgctgttc gtgctcggct gcctggccat cggcttcgtg ctggcggtgc tgatcgacca
     6121 gcgcgtgcgc ggcgaaggcc tgctgcgcac ggtgttcctc tatccgtacg cgatgtcctt
     6181 cgtcgccacc ggcctggtct ggcagtggat gctcaacccc gagctcgggc tgcaggccac
     6241 ggtgcgcggc tggggctgga cgggcttcac cttcgactgg atcgtgcgcc aggatctcgt
     6301 gatggtggcc atcgcgatcg ccgcgatctg gcaggcctcg gggctcgtca tggcgctgat
     6361 gctcgccggg ctgcgcggca tcgaggaaag cctgtggatg gccgcgcgca tcgacggcat
     6421 cccggcctgg cgcacctacc tcagcatcgt cgcgccacag ctcgggccct cgttcgcgac
     6481 ggccttcgtg ctgctcgcgg tgggcgtggt gaaggtgttc gacgtcgtcg tcgcgatgac
     6541 acagggcggc cccggcctcg cgagcgaggt gccggccaag ttcatcatcg acaacctctt
     6601 cggacgcgcc aacatcggcc tcgcctcggc ggcctcgacg gtgctgctgc tgaccgtgct
     6661 ggcgctgatc gcccccggcc tgtacctgcg ccggcgcggc gcgcggcgcg acgaggcgca
     6721 ccggccgcag gccgccgagg cgctgccgg
//
LOCUS       DASVLN010000842         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_529364, whole genome shotgun sequence.
ACCESSION   DASVLN010000842 DASVLN010000000
VERSION     DASVLN010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529364"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..691
                     /locus_tag="VF453_19880"
     CDS             281..691
                     /locus_tag="VF453_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX7689976.1"
                     /translation="MPTSAPVPVALTVHQQSRVLEIAFDDGASFRIPFELMRVYSPSA
                     EVQGHGPGQETLQTGKRDVVVTAIEPVGHYAIKPTFSDGHDSGLFTWDYLHRLGREQD
                     ALFAQYLERLAAAGLDRDAPMAPPGGGACASHPH"
     gene            711..1442
                     /gene="ubiE"
                     /locus_tag="VF453_19885"
     CDS             711..1442
                     /gene="ubiE"
                     /locus_tag="VF453_19885"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX7689977.1"
                     /translation="MTQTHFGYETVDETEKAGRVRGVFDSVARKYDVMNDLMSGGLHR
                     AWKKYTVAVANLKAGDRALDIAGGTGDLARAFARRVGPTGVVVHTDINEAMLRVGRDR
                     LLDEGLVLPTTICDAEKLPFPTGAFDLVSVAFGLRNMTHKDVALAEMNRVLRPGGRLL
                     VLEFSRIAEPLRKAYDWYSFNVLPRVGKAVAGDADSYRYLAESIRMHPDQATLKAMMK
                     TAGFGHVDVHNMAAGAVALHVGIKC"
     gene            1459..2484
                     /gene="galE"
                     /locus_tag="VF453_19890"
     CDS             1459..2484
                     /gene="galE"
                     /locus_tag="VF453_19890"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX7689978.1"
                     /translation="MATILLTGAAGYIASHTWLELLAAGFDVVGVDDFSNSSPEVLNR
                     LAELSGRRIDFERASVCDADAMDDLFERRRIDAVVHFAAFKAVGESTQQPLRYYANNI
                     GGLMTLAGAMQAHGTSRIVFSSSATVYGQPEILPIREDAPVRATNPYGETKLMGEAIL
                     RDMAAADPDWQVATLRYFNPVGAHESGRIGEDPRGVPNNLMPYVAQVAVGRRPRLAVF
                     GNDYPTPDGTGVRDYIHVVDLAQGHVAALHRLLSAPGSFTVNLGTGRGYSVLELIRAY
                     EKASGRAVPYEVVARRPGDVAACYADPARARELLDWSARRDLDAMCADSWRWQSLNPD
                     GFDGTAA"
BASE COUNT          416 a         1022 c          954 g          405 t
ORIGIN      
        1 accggcgtcg tgccgggcgg gcaggtgccg tcgccgtaac cgatgccatg cgggccgcgg
       61 caacgcagga tctgcgccgc cgcgaggccg gggatcgcca gcgcgcaggc cagcgccagc
      121 gcgcccaggc ggcgcgtcgt cgtcgtgtgc atttccctcc ctgtcgtgtt cttcgtggtc
      181 gcgggcgcca ttgtcaccgt ttgtcacgac ttgtcgatcc cccaacgcct gcttgcgggg
      241 ggtcaacggc ccggtcgccg acgacggcta aactcccggg atgccgacct ccgcccccgt
      301 tcccgtcgcg ctcacggtac accagcagtc ccgggtcctc gagatcgcat tcgacgacgg
      361 cgcgagcttt cgcatcccgt tcgagttgat gcgggtgtac tcgccctcgg ccgaggtgca
      421 gggccacggg ccgggccagg agacgctgca gaccggcaag cgcgacgtcg tcgtcaccgc
      481 catcgagccg gtcggccact acgcgatcaa gcccacgttc tccgacggcc acgactcggg
      541 cctgttcacg tgggactacc tccaccgcct cggccgcgag caggacgcgc tcttcgcgca
      601 atacctcgag cgcctggccg ccgccggcct cgaccgcgac gcgcccatgg ctccgcccgg
      661 cggcggtgcc tgcgcgtccc acccacactg aatcgcccag gatccgcccg atgacccaga
      721 cccatttcgg ctacgagacc gtcgacgaga ccgagaaggc cggccgcgtg cgcggcgtgt
      781 tcgactcggt ggctcgcaag tacgacgtga tgaacgacct catgtcgggc ggcctgcacc
      841 gcgcgtggaa gaagtacacc gtcgcggtgg ccaacctcaa ggccggcgac cgcgcgctcg
      901 acatcgcggg cggcaccggc gacctcgcgc gcgccttcgc gcgccgcgtc ggcccgaccg
      961 gcgtcgtcgt gcacaccgac atcaacgagg cgatgctgcg cgtcggccgc gaccgcctgc
     1021 tcgacgaggg cctggtgctg ccgacgacga tctgcgacgc cgagaagctg cccttcccga
     1081 cgggcgcgtt cgacctcgtg agcgtggcct tcggcctgcg caacatgacg cacaaggacg
     1141 tcgcgctcgc ggagatgaac cgcgtgctgc gcccgggcgg ccgcttgctg gtgctcgagt
     1201 tctcgaggat cgcggagccg ctgcgcaagg cctacgactg gtattcgttc aacgtgctgc
     1261 cgcgcgtggg caaggccgtc gcgggcgatg ccgacagcta ccgctacctc gccgagtcga
     1321 tccgcatgca tcccgaccag gcgacgctca aggcgatgat gaagacggcc ggcttcggcc
     1381 acgtcgacgt ccacaacatg gccgcgggcg ccgtggcgct gcatgtcggc atcaagtgct
     1441 gaagggcccg cgcacgccat ggccacgatc ctgctgaccg gcgcggccgg ctacatcgcc
     1501 tcgcacacct ggctggagct cctcgccgcg ggcttcgacg tcgtgggcgt cgacgacttc
     1561 tccaacagct cgcccgaggt gctcaaccgg ctggccgagc tgtccggccg ccggatcgac
     1621 ttcgagcgcg ccagcgtgtg cgacgccgac gccatggacg acctgttcga gcgccgccgg
     1681 atcgacgcgg tcgtgcactt cgcggcgttc aaggcggtgg gcgagtcgac gcagcagccg
     1741 ctgcgctact acgcgaacaa catcggcggg ctgatgacgc tcgccggcgc gatgcaggcg
     1801 cacggcacgt cgcgcatcgt cttcagctcc agcgccaccg tctacggcca gcccgagatt
     1861 ctgccgatcc gcgaggacgc gcccgtgcgc gcgaccaatc cctacggcga gaccaagctg
     1921 atgggcgagg ccatcctgcg cgacatggcc gcggccgacc cggactggca ggtcgcgacg
     1981 ctgcgctact tcaacccggt gggcgcgcac gagagcggcc gcatcggcga ggatccgcgc
     2041 ggcgtgccca acaacctgat gccctacgtg gcccaggtcg ccgtggggcg gcggccccgg
     2101 ctggcggtct tcggcaacga ctacccgacc cccgacggca ccggcgtgcg cgactacatc
     2161 cacgtcgtcg acctcgcgca ggggcacgtg gccgcgctgc accggctgct gtcggcgccc
     2221 ggctcgttca cggtcaacct cggcaccggg cgcggctaca gcgtgctgga gctgatccgc
     2281 gcgtacgaga aggcaagcgg ccgcgccgtc ccgtacgagg tcgtggcgcg ccggccgggc
     2341 gacgtcgcgg cctgctacgc ggatccggcg cgcgcgcgcg agctgctcga ctggtcggct
     2401 cgccgcgacc tcgacgccat gtgcgcggac agctggcgct ggcagtcgct gaacccggac
     2461 ggcttcgacg ggacggcggc gtgaggagga cgggcggcgt gcgcgatgcc ggcgtctgag
     2521 aaggtgcgaa cgaatccacc atgcccgctc gtgccgccaa atcgcgccca ctcgtcgttg
     2581 gaaatgctcg ccgtagcccg agctacgtct gcgctttccg cctagattgg acgcgatttg
     2641 gcgacccact cggacccgct ggattcattc acaccttctg agagggtgta gctgaaatga
     2701 tctacgcctg cgccaatcgc gaagcgagaa ggcgggcctc ggtcagccac acccatgcga
     2761 cgggcgccca gttggagcga tcgtgatgga ccatcaa
//
LOCUS       DASVLN010000843         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_420106, whole genome shotgun sequence.
ACCESSION   DASVLN010000843 DASVLN010000000
VERSION     DASVLN010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420106"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VF453_19895"
     CDS             complement(<1..154)
                     /locus_tag="VF453_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7689979.1"
                     /translation="MKSFSFERAGTPAEAAAAVARSPDAKFIAGGTNLLDLMKLQVEA
                     PRHLVDV"
     gene            complement(151..795)
                     /gene="paoA"
                     /locus_tag="VF453_19900"
     CDS             complement(151..795)
                     /gene="paoA"
                     /locus_tag="VF453_19900"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HEX7689980.1"
                     /translation="MDRFPPLPVTRRDMLVAGATSAVALNAQAEPARGADPEAVHTAR
                     VALEVNGQPRELELDTRTTLLDALREHLHLTGTKKGCDHGQCGACTVLLDGRRVNSCL
                     TLAVMHPHAKVTTIEGLGRPGELHPLQAAFVRHDGYQCGYCTPGQICSAVGMLDELRQ
                     GMPSHATVDIAAAPSLSNAELRERMSGNICRCGAYSNIAEAITEVAASANGGAA"
     gene            complement(849..1370)
                     /locus_tag="VF453_19905"
     CDS             complement(849..1370)
                     /locus_tag="VF453_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689981.1"
                     /translation="MRAKKLSATFRHPIYSPKGGVEGLLAEVDGVCVQVVLEKDDEAA
                     AALVGVLRAGQSLSLDAAPAEPSKKKGEPAHAVWALRKVLRVDGKAPKAAKPHAQGYS
                     GRIVRLNYARHGAPNGYVLDTGDFVHVKPEGFEKLGLDVGDQVDADGDAYFLSTGGGW
                     AIEADTVNGRAVK"
     gene            complement(1469..>2164)
                     /locus_tag="VF453_19910"
     CDS             complement(1469..>2164)
                     /locus_tag="VF453_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX7689982.1"
                     /translation="DSVELPDDGPARAPARLAPGTRIDAADREVVVHMRTDDPVVATL
                     GNVLDADECRALIELARPRLKPSTLVDPMTGRDVVSERRTSSGMFFRLEEDAFIARID
                     RRLSRLMNLPVDHGEGLQVLHYPEGAGSEPHFDYLQPTNAANRESVLRSGQRVSTLVT
                     YLADVPAGGQTVFPALGWVVSPLRGNAVYFEYGDDHGRVDARSLHASAPVTRGEKWVV
                     TKWMRARRFVPAG"
BASE COUNT          273 a          766 c          803 g          322 t
ORIGIN      
        1 tgacgtcgac gagatgccgc ggcgcctcga cctgcagctt catgaggtcc agcaggttgg
       61 tgccaccggc gatgaacttc gcgtcgggcg agcgcgcgac cgcggccgcc gcctcggccg
      121 gcgttccggc gcgctcgaag gagaaggact tcatgccgcg cctccgttcg cggatgccgc
      181 gacttcggtg atcgcctcgg cgatgttcga ataggcgccg cagcggcaga tgttgccgct
      241 catgcgctcg cgcagctccg cgttggacag gctcggcgcc gcggcgatgt cgacggtcgc
      301 gtggctcggc atgccctggc gcagctcgtc gagcatgccg acggcggagc agatctggcc
      361 cggggtgcaa tacccgcact ggtacccgtc gtggcggacg aacgcggcct gcagcgggtg
      421 cagttcgccg ggccggccca ggccctcgat cgtcgtgacc ttcgcgtgcg ggtgcatcac
      481 cgccagcgtc aggcagctgt tgacgcgccg gccgtcgagc agcacggtgc atgcgccgca
      541 ctggccgtgg tcgcatccct tcttggtgcc ggtcaggtgc aggtgctcgc gcagcgcgtc
      601 gagcagcgtg gtgcgcgtgt ccagctccag ctcgcgcggt tgaccgttga cctcgagcgc
      661 gacgcgtgcc gtgtgcacag cctcggggtc ggcgccgcgc gcgggctcgg cctgcgcgtt
      721 cagcgcgacg gcggacgtcg cgccggccac gagcatgtcg cgccgcgtga ccggcagggg
      781 agggaagcga tccatggcga tgcctcgcga agggggatcg cgtccgtcgg caagcggtgc
      841 gcctgcggct acttgacggc gcggccgttg acggtgtcgg cctcgatcgc ccagccgccg
      901 ccggtcgaca ggaagtaggc gtcgccgtcc gcgtccacct ggtcgccgac gtccagcccc
      961 agcttctcga agccctcggg cttcacgtgc acgaagtcgc cggtgtcgag cacatagccg
     1021 ttcggggcgc cgtgacgggc gtagttcagc cgcacgatcc tgcccgaata gccctgggca
     1081 tgcggcttcg cggccttggg cgccttgccg tcgacgcgca gcaccttgcg cagcgcccag
     1141 accgcgtggg ccggctcgcc cttcttcttc gacggctccg cgggcgcggc gtcgagcgac
     1201 aacgactgtc ccgcacgcag cacgccgacc agcgccgcgg cggcctcgtc gtccttctcg
     1261 agcacgacct ggacgcagac gccgtcgacc tcggcgagca ggccctcgac gccgcccttg
     1321 gggctgtaga tcgggtgccg gaacgttgcg gagagcttct tcgcgcgcat tgggcgggtc
     1381 cttcgtcggg gagatgcgcc acaggctacg cgagcgccgc cgcgcggtgc gtaggacaag
     1441 cgatgaacgc gtgcggccgc gcggccgctc agcctgcggg cacgaagcgc cgcgcgcgca
     1501 tccacttcgt cacgacccac ttctcgcctc gcgtcacggg cgcgctggcg tgcagcgagc
     1561 gcgcgtcgac gcgtccgtgg tcgtcgccgt actcgaagta gaccgcgttg ccgcgcagcg
     1621 gcgagaccac ccagccgagg gccgggaaca ccgtctggcc gccggcgggc acgtcggcca
     1681 ggtaggtcac gagcgtgctg acgcgctggc cgctgcgcag caccgactcg cggttggcgg
     1741 cgttggtcgg ctgcaggtag tcgaaatgcg gctcgctgcc ggcgccctcg ggatagtgca
     1801 gcacctgcag gccctcgccg tggtcgaccg gcaggttcat cagccgcgac agccggcggt
     1861 cgatgcgggc gatgaacgcg tcctcctcga gtcggaagaa catgcccgag ctcgtgcggc
     1921 gctcgctgac gacgtcgcgg ccggtcatcg ggtcgaccag ggtcgagggc ttcagccgcg
     1981 ggcgcgcaag ctcgatcagc gcgcggcatt cgtcggcgtc cagcacgttg cccagcgtcg
     2041 cgacgaccgg gtcgtcggtg cgcatgtgca cgacgacctc gcggtcggcc gcgtcgatgc
     2101 gcgtgccggg cgcgagccgc gcgggcgcgc gggcagggcc gtcgtcgggc agctcgacgc
     2161 tgtc
//
LOCUS       DASVLN010000844         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_259839, whole genome shotgun sequence.
ACCESSION   DASVLN010000844 DASVLN010000000
VERSION     DASVLN010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259839"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VF453_19915"
     CDS             complement(<1..299)
                     /locus_tag="VF453_19915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689983.1"
                     /translation="MAEPAGHRPFLREHAGVLVAAALAIAWSFAEPAMMGLPIVLGLL
                     WIVGWTTVASFVAWRRPQRRRALAQKAALYVAAMFAVGAIGGWHSERARRDADAA"
     gene            complement(292..1395)
                     /locus_tag="VF453_19920"
     CDS             complement(292..1395)
                     /locus_tag="VF453_19920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7689984.1"
                     /translation="MGLLQRANVALLTRDRNQRFRLAQAWLACILLFCCMLVLHLANG
                     FGLIDPGPVWGWSAASLAGMLAVIGVIRSGLNRRLRDPALTMPQMLYAVFCSAWGYTF
                     AQQVHTMVYLILAVILMFGMFGLSMRQLLWGSGYVLALFGGAMLMMTRRDPVHYPAGV
                     EICNFLMMIVMVVGVALLTERLHRLRDRLRRQKVELEAALAHIQRLATHDELTGLVNR
                     RHMQELLENERLRSERTTQDWCVALLDLDHFKAVNDAYGHAIGDEVLRALSRHARELV
                     RKTDVLARWGGEEFVLLLPNTPIATAAASLERLREHFHATPLQVREVVLPLSFSAGLT
                     QHQRGETFAQTLERADKLCYQAKSLGRNRIERG"
BASE COUNT          246 a          557 c          575 g          209 t
ORIGIN      
        1 atcgcggcgt cggcgtcgcg gcgcgcgcgc tccgagtgcc agccgccgat cgcgccgacc
       61 gcgaacatcg ccgcgacgta gagcgccgcc ttctgcgcga gggcccggcg ccgctgcggc
      121 cggcgccaag cgacgaagga cgcgacggtc gtccagccga cgatccacag cagcccgagc
      181 acgatgggca ggcccatcat cgcgggctcg gcgaaggacc aggcgatcgc cagcgccgcc
      241 gcgacgagca cgccggcatg ctcgcgcagg aagggccggt ggccggcggg ctcagccacg
      301 ttcgatccgg ttgcggccca gcgacttggc ctggtagcag agcttgtcgg cgcgttccag
      361 cgtctgcgcg aaggtctcgc cacgctggtg ctgcgtgagg ccggccgaga acgacagcgg
      421 cagcacgacc tcgcgcacct gcagcggcgt cgcgtggaaa tgctcgcgca ggcgctcgag
      481 cgacgccgcg gcggtcgcga tcggcgtgtt gggcagcagc agcacgaact cctcgccgcc
      541 ccaacgcgcg agcacgtcgg tcttgcgtac cagctcgcgc gcgtggcggc tgagcgcgcg
      601 cagcacctcg tcgccgatcg cgtggccgta ggcgtcgttg accgccttga agtggtcgag
      661 gtccagcagc gccacgcacc agtcctgcgt ggtgcgctcg ctgcgcaggc gctcgttctc
      721 gagcagctcc tgcatgtggc ggcggttgac gaggccggtg agctcgtcgt gcgtggccag
      781 gcgctggatg tgcgcgaggg cggcctcgag ctcgaccttc tggcgccgca ggcggtcgcg
      841 caggcggtgc aggcgctcgg tcagcagcgc cacgccgacg accatgacga tcatcatcag
      901 gaagttgcag atctccacgc ccgccgggta gtggacggga tcgcgtcgcg tcatcatcag
      961 catggcgccg ccgaacaggg ccaggacgta gcccgatccc cacaggagct ggcgcatgct
     1021 gagcccgaac atgccgaaca tcaggatgac ggccaggatc aggtacacca tggtgtgcac
     1081 ctgctgcgcg aaggtgtagc cccaggccga gcagaagacc gcgtagagca tctgcggcat
     1141 cgtcagcgcc gggtcgcgca gccggcggtt gaggccgctt cggatcacgc cgatcaccgc
     1201 cagcatgccg gccagcgagg ccgccgacca cccccacacc ggccccggat cgatcagccc
     1261 gaacccgttg gccaggtgca gcaccagcat gcagcagaac agcaggatgc acgcgagcca
     1321 cgcctgcgcc aggcggaagc gctggttgcg gtcacgcgtg agcagggcga cgttcgccct
     1381 ctggagcagt cccatgtccc ctggatgtcg tggttcagcg ccgggatttc ggcaacttcc
     1441 ccgggaaggg tagcggagat tcggtgcccg cgggcaggga aagttgcagc atttcacgca
     1501 gcgccgcggg tttcgcgccc ggccgcgtgg gccggcgcgt ccgcgcgggc gccagcagct
     1561 cggccatgtc ccgtgcgccg gcgcgac
//
LOCUS       DASVLN010000845         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_205112, whole genome shotgun sequence.
ACCESSION   DASVLN010000845 DASVLN010000000
VERSION     DASVLN010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205112"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VF453_19925"
     CDS             <1..1587
                     /locus_tag="VF453_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7689985.1"
                     /translation="ASMPGRPLAAAAGPTRADCGAKLFMATADARVIALDPGSGAVCT
                     RFGGGTGQIDLAAHMPNLHPGSYYSTSPPVVTKRLLVVGGTVLDNYSTHEESGVVRAF
                     DVDTGALVWNWDSGNPDATAPIAAGGTYTANSPNSWSIASVDEALGLVYLPMGNQPPD
                     QFGGHRSAAVERVSSSVVALDLATGKLRWVFQTVHHDLWDYDVPAQPTLVDLDIHGER
                     VPALVQPTKQGELFVLDRRTGVPVVPVTEEPAPDGAAAGDHSAPTQPKSGLSFEPAPL
                     TEASMWGGTILDQTLCRIAFRKLRYGGRFTPPSTQGSLIYPGNFGVFNWGGIAVDPRT
                     GTAFMMPTYLAFTSTLVPRPDASTLLVQTPPGRKPPGALPPLNENFGAPYAAKIGPFL
                     SPLGLPCQAPPWGYVAGADLRTGRIAWRHRNGTVRDLAPIPLPFRMGVPGIGGPILTG
                     GGVAFLSGTMDDYVRGYDAATGRELWRARLPAGGQATPMTYAAADGRQMLLVVAGGHG
                     STHTRPGDYVIAYALPAREH"
     gene            1590..>2103
                     /locus_tag="VF453_19930"
     CDS             1590..>2103
                     /locus_tag="VF453_19930"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX7689986.1"
                     /translation="MAASAKRVRGGAPSLTALPLRDADDGDFLAVIETARGSANKLKY
                     DEALGAFRLGHVLPPGLVFPFDFGFLPSTRGEDGDPLDVVVLMDEPAAAGVVVPCRVV
                     GVIRATQAEPPSPERTRNDRFVAVAARSHRWAGCEELDDLPAALLDAFERFCVDYEAR
                     RGIDFRPEGRD"
BASE COUNT          260 a          820 c          736 g          287 t
ORIGIN      
        1 gcctccatgc ccggccgccc gctcgccgcc gccgccgggc ccacgcgcgc ggactgcggc
       61 gccaagctgt tcatggcgac cgccgacgcg cgcgtcatcg cgctcgatcc cggctccggc
      121 gccgtctgca cgcgcttcgg cggcggcacc ggccagatcg atctcgccgc ccacatgccc
      181 aacctgcatc cgggctcgta ctactccacc tcgccgccgg tcgtcacgaa gcggctgctg
      241 gtcgtcggcg gcaccgtgct cgacaactac tcgacgcacg aggagtccgg cgtcgtccgc
      301 gcgttcgacg tcgacaccgg tgcgctcgtg tggaactggg attccggcaa cccggacgcg
      361 acggccccga tcgccgcggg cggcacctac accgcgaact cgcccaacag ctggtcgatc
      421 gccagcgtcg acgaggcgct cggcctcgtc tacctgccga tgggcaacca gccgcccgac
      481 cagttcggcg gccaccgcag cgcggcggtc gagcgcgtct cgtcctccgt cgtggcgctc
      541 gacctggcga cgggcaagct gcgctgggtc ttccagaccg tgcaccacga cttgtgggac
      601 tacgacgtgc cggcgcagcc gacgctggtc gacctcgaca tccacggcga gcgcgtgcct
      661 gcgctggtgc agccgaccaa gcagggcgag ctgttcgtac tggatcgtcg caccggcgtg
      721 ccggtcgtgc cggtgaccga ggagcccgcc cccgacggcg ccgcggcggg cgaccactcg
      781 gcgccgacgc agccgaagtc gggcctgtcc ttcgagcccg cgccgctcac cgaggcgagc
      841 atgtggggcg gcacgatcct cgaccaaacg ctgtgccgca tcgccttccg aaagctgcgc
      901 tacggcggcc gcttcacgcc gccgtcgacg cagggatcgc tgatctaccc gggcaacttc
      961 ggcgtcttca actggggcgg gatcgccgtc gatccgcgca ccggcacggc gttcatgatg
     1021 ccgacctacc tcgccttcac gtcgaccctg gtgccgcgac cggatgcgag cacgctgctg
     1081 gtccagacgc cgccgggccg gaagccgccc ggcgcgctgc cgccgctcaa cgagaacttc
     1141 ggcgcgcctt acgcggcgaa gataggcccg ttcctctcgc cgctgggcct gccctgccag
     1201 gcgccgccct ggggctacgt cgcgggcgcc gacctgcgca ccgggcgcat cgcctggcgc
     1261 caccgcaacg gcacggtgcg cgacctcgcg cccatcccgc tgccgttccg gatgggggta
     1321 ccgggtatcg gcgggcccat cctcacgggc ggcggcgtgg cgttcctgtc cgggacgatg
     1381 gacgactacg tccgggggta tgacgcggcg accggccgcg agctgtggcg cgcccgcctg
     1441 cccgcgggcg gccaggcgac gccgatgacc tacgcggccg ccgacggccg ccagatgctg
     1501 ctggtggtcg cgggcggcca cggctcgacg cacaccaggc cgggcgacta cgtcatcgca
     1561 tacgccttgc cggcgcggga gcattgacga tggccgcgtc cgcgaagcgg gtccgcggtg
     1621 gcgcgccgtc gctgaccgcc ctgcccctgc gcgacgccga cgacggcgac ttcctcgccg
     1681 tcatcgagac cgcccgcggc tcggccaaca agctcaagta cgacgaggcc ctgggcgcgt
     1741 tccggctcgg gcacgtgctg ccgcccgggc tcgtcttccc gttcgacttc ggcttcctgc
     1801 cttcgacgcg cggcgaggac ggcgacccgc tggacgtcgt cgtgctgatg gacgagccgg
     1861 ccgccgccgg cgtcgtggtg ccgtgccgcg tcgtcggcgt gatccgcgcg acgcaggccg
     1921 agccgccgtc gcccgagcgc acgcgcaacg accgcttcgt cgcggtggct gcgcgatcgc
     1981 accgctgggc gggctgcgag gagctcgacg acctgcccgc cgcgctcctg gacgccttcg
     2041 agcgcttctg cgtcgactac gaggcgcggc ggggcatcga cttccggccc gagggccgcg
     2101 acg
//
LOCUS       DASVLN010000846         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_155389, whole genome shotgun sequence.
ACCESSION   DASVLN010000846 DASVLN010000000
VERSION     DASVLN010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155389"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VF453_19935"
     CDS             <1..971
                     /locus_tag="VF453_19935"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX7689987.1"
                     /translation="AATVAPELELFLVDRHEVDGRVELQAPAARPDAPARERACESYS
                     LERASHFAGYFDELFAACALQGIPACGHAHEAAFSQYEVNFQPGAPLAQADAVFRFKR
                     LARQVAARFGFLATFAPKPFLDQPGTGLHWHFSLQRRDAEPGAHDNAFAEPDGSDRPA
                     LGAFIGGLQAHADAAMAFFAPHDMAFDRIRLSNASPSSASWAHDDRGVAFRIPQASAR
                     NRRVENRLPGGDANPYLTVAATLGMGLAGIEQGLSPAGLAEGVALPRSLPEACDALER
                     SSLMRELFGDPLVDLFVALKRHESAERNACADPRHHWDLVHLLELA"
     gene            1001..2293
                     /locus_tag="VF453_19940"
     CDS             1001..2293
                     /locus_tag="VF453_19940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7689988.1"
                     /translation="MFRSAPDYTDTYYAATSRHLQTERAPLQEDLDVDVCVVGAGFFG
                     LYTALELAQAGKRVAILEASRVGWGASGRNGGQMIIGFPCGMQRLKGVMGQERAKRMF
                     DASREAVSDIRRIMRDNAIDCDLVEGHLEVAVLPRRVADLTGWIEECENEWGYRKLQL
                     VEKKDLPKYLNSPRFQAGIIDPEGAHIHPLKYVLGLARAVEAAGVRIFERSKVEGYAE
                     NADHILVRSGDHVVRCHQLVLAANAYVDKLDPRLAARTLPVGTFIATTAPLGEKLARE
                     LIPHNHAVYDNQFILEYFRMTADHRLLFGGKCTYLGGTPVDLPNAMKRNIRRVFPQLA
                     DAKIDFAWGGHIDITMRRMPDWGRRGDRIFWAQGFCGHGLVPTRVAGKLVSKAVLGDT
                     EELDWFAPIVNPPFPGGEALGGLMQAVGMSWYRVRDFV"
     gene            2368..3012
                     /locus_tag="VF453_19945"
     CDS             2368..3012
                     /locus_tag="VF453_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX7689989.1"
                     /translation="MKIDFVSDVSCPWCAIGLSSLEIALARVGAEVPVELHFQPFELN
                     PGMAGEGEDVTEHLSKKYGMPPEQVAANGERIRERGAAVGFAFDMRARGRIYNTFDAH
                     RLLHWADTQGEGRQLALKKALLKAYFTDGQDPSSHDTLVRYAESVGLDGARAREVLAS
                     GAYANEVREAEAWYQQRGIHSVPAIIIDDRHLISGGQPPEVFEQALRQLASQPA"
     gene            complement(3044..3463)
                     /locus_tag="VF453_19950"
     CDS             complement(3044..3463)
                     /locus_tag="VF453_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7689990.1"
                     /translation="MDTLDQRLIALLRTDARASVATLAHRLGVSRGTVNNRIAKLEDA
                     GTIVGYTVRLRPDIEHNEIVAWMSIAVEGNQTREVIARLLGEPGVGELHDTNGRWDLL
                     AEIRCANLSDLAAVLERIRLIKGIGATETSIHLQSYK"
     gene            3607..4668
                     /locus_tag="VF453_19955"
     CDS             3607..4668
                     /locus_tag="VF453_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HEX7689991.1"
                     /translation="MTTLLTTRDIGAIVAAKGLGAVLGTLVAYLEQDFARWAEFDKSA
                     RVANHSPDGVIELMPISDASLYTFKYVNGHPKNTRAGLPTVMAFGVLADVDTGRPELL
                     SELTLTTAMRTAATSVMVARRLARPDSRVMALVGNGAQSEFQALAFHLLLGISEVRLF
                     DIDPGATAKLVANLHAAAPGLKTTVCASTAEAVRGADIVTTVTADKTNATILTPDMIA
                     PGMHVNGVGGDCPGKTELHADVLRAGPVFVEYEPQSRVEGDVQQMPADFPVTEFWRVL
                     AGTAPGRRTADEVTVFDSVGFALEDFSALRLLRDLAAELGLGRETDLVPDMADPKNLF
                     GELRLPVSPAARASSAIAA"
BASE COUNT          680 a         1739 c         1615 g          673 t
ORIGIN      
        1 tggccgcgac cgtcgcgccg gagctggagt tgttcctcgt cgaccgccac gaggtcgacg
       61 gccgcgtcga gctccaggcg cccgccgcgc gcccggacgc gcccgcgcgc gagcgggcct
      121 gcgagtccta ctcgctcgag cgcgccagcc acttcgccgg gtacttcgac gagctgttcg
      181 ccgcctgcgc gctgcagggc atcccggcct gcggccatgc gcacgaggcc gcgttctcgc
      241 agtacgaggt caacttccag cccggcgcgc cgcttgcgca ggccgacgcg gtgttccgtt
      301 tcaagcgcct cgcgcgccag gtcgcggcgc gcttcggctt cctcgcgacc ttcgcgccca
      361 agcccttcct cgaccagccc ggcaccggcc tgcattggca cttcagcctc cagcggcgcg
      421 acgccgagcc gggggcgcac gacaacgcct tcgccgagcc cgacggctcc gaccggccgg
      481 cgctgggcgc cttcatcggc ggcctgcagg cgcatgccga cgcggcgatg gccttcttcg
      541 ccccgcacga catggcgttc gaccgcatcc gcctgagcaa cgcgtcgcca tcgtcggcga
      601 gctgggccca cgacgaccgc ggcgtcgcct tccgcatccc gcaggcgagc gcgcgcaacc
      661 gccgcgtcga gaaccgcctg cccgggggcg acgccaatcc gtacctgacc gtcgccgcca
      721 cgctgggcat gggcctggcc ggcatcgagc aggggctctc gccggccggc ctggcggagg
      781 gagtcgcgct cccgcgctcg ctgcccgagg cgtgcgacgc gctcgagcgc agttcgctga
      841 tgcgcgagct cttcggcgac ccgctggtcg acctgttcgt cgcgctcaag cgccacgaga
      901 gcgcggagcg caacgcctgc gccgacccgc gacaccactg ggatctcgtg cacctgctcg
      961 agctcgcctg accccgcaga caccgcaacg aggccttcgc atgttccgct ccgcccccga
     1021 ctacaccgac acctactacg ccgcgacgtc gcgccacctg cagaccgagc gcgcgccgct
     1081 gcaggaggac ctggacgtcg acgtctgcgt cgtcggcgcc ggcttcttcg ggctgtacac
     1141 cgcgctggag ctcgcgcagg cgggcaagcg cgtggcgatc ctcgaggcga gccgcgtcgg
     1201 ctggggcgcc tcgggccgca acggcggcca gatgatcatc ggctttccct gcggcatgca
     1261 gcgcctgaag ggcgtgatgg gccaggagcg cgcgaagcgc atgttcgacg cctcccgcga
     1321 ggcggtgagc gacatccgcc gcatcatgcg cgacaacgcg atcgactgcg acctcgtcga
     1381 gggccacctc gaagtcgcgg tgctgcctcg ccgcgtcgcc gacctcacgg gctggatcga
     1441 ggaatgcgag aacgagtggg gctaccgcaa gctgcagctg gtcgagaaga aggacctgcc
     1501 gaagtacctg aactcgccgc gcttccaggc cggcatcatc gatcccgagg gcgcgcacat
     1561 ccacccgctg aagtacgtgc tgggcctcgc gcgcgcggtc gaggccgcgg gcgtgcgcat
     1621 cttcgagagg agcaaggtcg agggctacgc cgagaacgcc gaccacatcc tcgtgcgctc
     1681 gggcgaccac gtggtgcgct gccaccagct ggtgctcgcg gccaacgcct acgtcgacaa
     1741 gctcgacccc cggctcgccg cccgcacgct gcccgtgggc accttcatcg cgacgaccgc
     1801 gccgctgggc gagaagctcg cgcgcgagct gatcccgcac aaccacgcgg tctacgacaa
     1861 ccagttcatc ctcgagtact tccgcatgac ggccgatcat cggctgctgt tcggaggcaa
     1921 gtgcacctac ctgggtggca cgccggtcga cctgcccaac gcgatgaaac gcaacatccg
     1981 ccgcgtcttc ccgcagctgg ccgacgcgaa gatcgacttc gcctggggcg gccacatcga
     2041 catcaccatg cgccgcatgc ccgactgggg ccgccgcggc gaccgcatct tctgggccca
     2101 gggcttctgc ggccatggcc tcgtcccgac gcgggtggcg ggcaagctcg tctcgaaggc
     2161 ggtgctgggc gacaccgagg agctcgactg gttcgccccc atcgtcaacc cgccgtttcc
     2221 cggcggcgag gcgctgggcg gcctgatgca ggcggtcggg atgagctggt accgcgtgcg
     2281 cgatttcgtc tgagccaaag cccgcaccgg ccctcggact cgcggcgcga tcgacaatcc
     2341 ggtcctcccc gtcctggaag gcccgccatg aagatcgatt tcgtctccga cgtctcctgc
     2401 ccctggtgcg cgatcgggct gtcgtcgctc gagatcgcgc tcgcgcgcgt cggcgccgag
     2461 gtgccggtgg aactgcactt ccagcccttc gagctgaatc ccggcatggc gggcgagggc
     2521 gaggacgtca ccgaacacct gtcgaagaag tacggcatgc cgcccgagca ggtggcggcc
     2581 aacggcgagc gcatccgcga gcgcggcgcc gccgtgggct tcgcgttcga catgcgggcg
     2641 cgcggccgca tctacaacac cttcgacgcc catcgcctgc tgcactgggc cgacacgcag
     2701 ggcgagggcc ggcagctcgc gctcaagaag gcgctgctga aggcctactt caccgacggc
     2761 caggatccga gctcgcacga cacgctcgtg cggtacgcgg agtcggtcgg cctcgacggc
     2821 gcgcgggcgc gcgaggtgct cgcgagcggc gcctacgcga acgaggtgcg cgaggccgag
     2881 gcgtggtacc agcagcgcgg catccactcg gtgccggcca tcatcatcga cgaccgccac
     2941 ctgatctccg gcggccagcc gcccgaggtg ttcgagcagg cgctgcggca gctcgcctcg
     3001 cagccggcgt gacgccgcac ggcgccgggc cggccggcgc ggttcacttg tagctctgca
     3061 ggtggatgct ggtctcggtc gcgccgatgc ccttgatcag ccggatgcgc tccagcactg
     3121 ccgcgaggtc ggaaaggttg gcgcagcgga tctcggccag caggtcccag cggccgttgg
     3181 tgtcatgcaa ctcgcccacg cccggctcgc cgagcagccg ggcgatcacc tcgcgcgtct
     3241 ggttgccttc gaccgcgatg ctcatccagg cgacgatctc gttgtgctcg atgtccggcc
     3301 gcagccgcac cgtgtagccg acgatggtgc cggcgtcctc gagcttggcg atgcggttgt
     3361 tgaccgtgcc gcgcgagacg ccgagccggt gcgcgagggt ggcgaccgag gcccgggcgt
     3421 cggtgcgcag cagggcgatg agccgttggt ccagggtgtc catggggtcg tgccgacgct
     3481 ttctgatcga aacgccagtg cctgctgccg ttttggcaga ttattgatcg aaacgaggca
     3541 aagttgccca tttcgattgc cgagggcggg cgaaacaatc tccatgaaga caacggagac
     3601 attcccatga cgaccttgct caccacccgc gacatcggcg cgatcgtcgc cgccaagggc
     3661 ctgggcgccg tcctcggcac gctcgtggcc tacctcgagc aggacttcgc ccgctgggcc
     3721 gagttcgaca agagcgcccg cgtggccaac cactcgcccg acggcgtgat cgagctgatg
     3781 ccgatctccg acgcgagcct gtacacgttc aagtacgtca acggccaccc gaagaacacg
     3841 cgcgccggcc tgcccacggt gatggccttc ggcgtgctgg ccgacgtcga caccggccgc
     3901 ccggagctcc tgagcgagct cacgctgacc accgccatgc gcaccgcggc gacctccgtc
     3961 atggtcgcca ggcgcctcgc gcgtcccgac agccgcgtga tggcgctggt cggcaacggc
     4021 gcgcagagcg agttccaggc gctggcgttc cacctgctgc tcggcatttc cgaggtgcgc
     4081 ctgttcgaca tcgacccggg cgcgacggcc aagctggtgg ccaacctgca cgccgcggcg
     4141 ccggggctga agaccacggt ctgcgcgagc accgccgagg ccgtgcgcgg cgccgacatc
     4201 gtcaccacgg tgaccgcgga caagaccaac gcgacgatcc tgacgcccga catgatcgcg
     4261 ccgggcatgc atgtcaacgg cgtcggcggc gactgcccgg gcaagaccga gctgcacgcc
     4321 gacgtgctgc gcgccggccc ggtcttcgtc gagtacgagc cgcagagccg cgtcgagggc
     4381 gacgtgcagc agatgccggc cgacttcccg gtcaccgagt tctggcgcgt gctcgccggc
     4441 acggcgccgg gccgccgcac ggccgacgag gtgacggtgt tcgactcggt cggcttcgcg
     4501 ctggaggatt tctcggccct gcgcctgctg cgcgacctcg ccgcggagct cggcctgggc
     4561 cgcgagacgg atctcgtgcc cgacatggcc gatccgaaga acctcttcgg cgagctgcgc
     4621 ctgccggtgt cgccggccgc gcgcgcgagc agcgccatcg cggcctgaat gccggccgcc
     4681 ggggctgtcg cgcacgcgac gcatgcc
//
LOCUS       DASVLN010000847         6839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_155422, whole genome shotgun sequence.
ACCESSION   DASVLN010000847 DASVLN010000000
VERSION     DASVLN010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155422"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VF453_19960"
     CDS             complement(<1..944)
                     /locus_tag="VF453_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7689992.1"
                     /translation="MKYTVAVRTLCEFTAKRGDLDLRFTPSATALEGMAGHALVAASR
                     GERYETEITLRGERGDLVVRGRADGFDPERGRLEEIKTYRGDLAAMPANRRALHWAQA
                     LVYGALLCAERGLAQLEVALVYFDVATQHETSLVETRTAQALRAFFEDACDRFLAWAA
                     QESAHRAARDAGLAALAFPHAQFRAGQRQLAEQVWRAATAGRTLLAQAPTGIGKTVGT
                     LFPQLKAMPGEKLDKLFFLAAKTPGRGLALHALETIAAGPAVAGAPRRAPIRILELVA
                     REKACEHPDKACHGESCPLAQGFYDRLPAAREAAVAPTT"
     gene            1106..1879
                     /locus_tag="VF453_19965"
     CDS             1106..1879
                     /locus_tag="VF453_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689993.1"
                     /translation="MSSGFILETRDLTKEFKGFVAVNKVNLRVRRGSIHALIGPNGAG
                     KTTCFNLLTKFLQPTSGTITFDGNDISREAPAAIARRGVIRSFQISAVFQHLSVLENV
                     RVALQRSLGTSFHFWKPERSLSRLDARAMELLAEVDLAAYAKLPAVELSYGRKRALEI
                     ATTLAMEPTLMLLDEPTQGMGTEDVDRIRQLIKKVGANRTILMVEHNMSVVSSIADTI
                     TVLQRGALLAEGSYAEVSSNPAVVEAYMGSANVELAGAH"
     gene            1899..2603
                     /locus_tag="VF453_19970"
     CDS             1899..2603
                     /locus_tag="VF453_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7689994.1"
                     /translation="MATPFLQIQGLNAWYGESHVLHGVDFHVDEGEVLTILGRNGAGR
                     TTTMRAIMGLTGARKGSIKVKGQETIHLPTHKVARLGLGYCPEERGIFSSLSTEENLM
                     LPPVLTPGGMPIEQIYAMFPNLAERRSSQGTRLSGGEQQMLAVARILRTGARLLLLDE
                     ISEGLAPVIVQKLAEMVVALRKQGYTVIMVEQNFHFAAPLADRFLVMEHGQVIQQFTQ
                     AELPSRKERLHEYLGV"
     gene            2687..3901
                     /locus_tag="VF453_19975"
     CDS             2687..3901
                     /locus_tag="VF453_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7689995.1"
                     /translation="MSIKHTLKSVAFACAAALGFAGSAHAQISGDVIRIGFITDLSGV
                     YADIDGPGGVEAIKMAIADAGGQINGKKIELLTADHQNKPDVAAAKAREWIDTQGVDM
                     IIAGTNSGTNLAMAKVAAEKHKLLISVGGGTSALTNEQCSPTVLHWAYDTTALAKGTG
                     SAVTKAGGKTWYFLQADYAFGQALESDTAKVVEANGGKVLGVVKHPLAASDFSSFLLQ
                     AQASKAQILGLANAGGDTINAIKAANEFGITKTMKLAGLLMFIQDVHSLGLKTTQGMY
                     LTDSWYWNKTPETRAWGRRFFDVMKKMPGSLHAGDYSAAANYLAAVKATGSDDTDKVL
                     AKMKATKVNDIFAKDGWIRGDGSMIHDMYLMQVKTPEASKEPWDYYNVVETIKGEAAW
                     TTKAESKCAAWK"
     gene            4096..4983
                     /locus_tag="VF453_19980"
     CDS             4096..4983
                     /locus_tag="VF453_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7689996.1"
                     /translation="MTEIFGVPIQALFGQLMLGLVNGSFYAILSLGLAVIFGLLGVVN
                     FAHGALYMIGAYVAWFLMDRWGLGFWWALALGPLAVGVLGAVIERLLLKRLYKLDPLY
                     GLLLTFGLALVFQGVLLQYYGSSGQSYDAPDALSGATNLGFMVLPNYRGFVIVASLVI
                     CLGTWFLIERTRLGALLRAGTENPSLVQAFGVNVPLMVTLTYAFGAALAGLAGVLAAP
                     VIQVTPLMGANLIIVVFAVVVIGGMGSIIGSIVTGLVLGLVEGLTRVFYPEASNVVVF
                     AIMAIVLMIRPAGLFGKEK"
     gene            5045..6001
                     /locus_tag="VF453_19985"
     CDS             5045..6001
                     /locus_tag="VF453_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7689997.1"
                     /translation="MNWLGNGTDRSGKLVWIAALAILIVAPFVGIYPIFVAEALCFAI
                     FAIAFNLLLGFTGLLSFGHAAFFGGAAYVCAWLLQDKHWTPELALLGGTAAAAAAGLV
                     VGAIAIRRQGIYFAMVTLALAQMLYFFFLQAPFTGGENGIQGIARGRLFGLIPLEHDL
                     ALYFFVLAVFVGVFLLALRIVHSPYGQVLKAIRENEPRAVSLGYNVDRYKLLAFVLSA
                     ALAGLGGGLKTLVLHLATLQDVRWEQSGEVILMTLLGGLGTYAGPVVGAFVVVGLQNY
                     LSDRAGSWTGVIIGAIFVLCVVAFRKGFVGELLAWAKRRARA"
     gene            6003..6236
                     /locus_tag="VF453_19990"
     CDS             6003..6236
                     /locus_tag="VF453_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689998.1"
                     /translation="MARGRTWFRARKFGWGWGLPNTWQGWLVYAAYALLLAGGWRAFP
                     LPHHAAAFAIWAAGLTALLAAICYATGDKPRWR"
     gene            complement(6237..6749)
                     /locus_tag="VF453_19995"
     CDS             complement(6237..6749)
                     /locus_tag="VF453_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7689999.1"
                     /translation="MTKSYVVSGQGSRYRDEALRVPDHVEIHFYFTRLKAPQIYKART
                     ILDRFMVELGMFPDWVATAGEQLPAYFCWNQPDGKVPSGVYRRSTGELAIPLDGTDAG
                     RPVSLAHLIQEVAGSRLREQKTTAIHWLVRCEDVPGSAAGLALQYPRRLRGGVVTDNA
                     ESAQVPLTRF"
BASE COUNT         1019 a         2405 c         2370 g         1045 t
ORIGIN      
        1 gtcgtcggcg cgaccgccgc ctcgcgtgcc gcgggcaggc ggtcgtagaa gccctgcgcg
       61 agcgggcagg actcgccgtg gcaggccttg tccggatgct cgcaggcctt ctcgcgggcg
      121 acgagctcga ggatgcggat cggcgcccgc cggggcgcgc cggccaccgc cggcccggcg
      181 gcgatcgtct ccagcgcatg cagcgcgagc ccgcgccccg gcgtcttggc ggcgaggaag
      241 aacagcttgt ccagcttctc gcccggcatc gccttcagct gcgggaacag cgtgcccacc
      301 gtcttgccga tgcccgtggg ggcctgcgcg agcagcgtgc ggcctgccgt cgccgcgcgc
      361 cagacctgct cggcgagctg gcgctggccg gcgcggaact gcgcgtgcgg gaacgcgagc
      421 gcggcaagcc cggcgtcgcg cgcggcgcgg tgcgcggact cctgcgcggc ccaggcgagg
      481 aaccggtcgc aggcgtcctc gaagaacgcc cgcagcgcct gcgcggtgcg cgtctcgacg
      541 agcgaggtct cgtgctgcgt cgcgacgtcg aagtagacga gcgcgacctc gagctgcgcc
      601 agcccgcgct cggcgcacag caacgcgcca tagaccagcg cctgcgccca gtgcagggcg
      661 cgccggttcg cgggcatcgc cgcaaggtcg ccgcggtagg tcttgatctc ctcgaggcgt
      721 ccgcgttcgg ggtcgaagcc gtcggcgcgg ccgcgcacga cgaggtcgcc gcgctcgccg
      781 cgcagcgtga tctcggtctc gtagcgctcg ccgcggctcg ccgcgaccag ggcatggccg
      841 gccatgcctt cgagcgcggt cgccgagggc gtgaaccgca ggtcgaggtc gccgcgcttg
      901 gccgtgaact cgcagagggt gcggacggct accgtgtact tcatccgggg caatcgtgtc
      961 gcgccgatga cggatccggc gcgagccgcg cacgtcgcgg cgcggcgcct cgattgttgc
     1021 aaaacccgga caggccgacc tggggttcac ccgaacgtac ttttgcgaac tgtcacgcct
     1081 agggcccaac ccctaaactc gtcggatgag cagtggattc atcctggaaa cgcgcgacct
     1141 cacgaaggag ttcaagggct tcgtggcggt caacaaggtc aacctgaggg ttcgccgcgg
     1201 cagcatccat gcgctgatcg gccccaacgg cgccggcaag acgacctgct tcaacctgct
     1261 gacgaagttc ctgcaaccca cctccggcac gatcaccttc gacggcaacg acatctcgcg
     1321 cgaggcgccc gccgcgatcg cgcgccgcgg cgtcatccgc tcgttccaga tctcggccgt
     1381 cttccagcac ctgtccgtgc tcgagaacgt gcgcgtcgcg ctgcagcgct cgctgggcac
     1441 ctcgttccac ttctggaagc ccgagcgcag cctgagccgg ctcgacgccc gcgcgatgga
     1501 gctgctcgcg gaggtcgacc tcgccgccta tgcgaagctg ccggccgtgg agctgagcta
     1561 cggccgcaag cgcgccctcg agatcgcgac gacgctcgcg atggagccga cgctgatgct
     1621 gctggacgag ccgacccagg gcatgggcac ggaggacgtc gaccgcatcc gccagctgat
     1681 caagaaggtc ggcgcgaacc gcaccatcct gatggtcgag cacaacatga gcgtggtgtc
     1741 gagcatcgcc gacacgatca ccgtcctgca gcgcggcgcg ttgctcgccg agggcagcta
     1801 cgccgaggtg tcgagcaatc ccgcggtggt cgaggcctac atgggctcgg ccaacgtcga
     1861 gctcgccggc gcccactgaa caccggaccc cagaccgcat ggccactccc ttcctccaga
     1921 tccagggcct gaacgcctgg tacggcgaat cgcacgtgct gcacggcgtc gacttccacg
     1981 tcgacgaggg cgaggtgctc acgatcctcg gccgcaacgg cgcgggccgc acgacgacca
     2041 tgcgcgcgat catgggcctg acgggcgcgc gcaaaggctc gatcaaggtc aagggccagg
     2101 agacgatcca cctgccgacg cacaaggtcg cgcgcctcgg cctgggctac tgccccgagg
     2161 agcgcggcat cttctcgagc ctgtcgaccg aggagaacct catgctgccg cccgtgctga
     2221 cgcccggcgg catgcccatc gagcagatct acgcgatgtt cccgaacctc gcggagcgca
     2281 ggtccagcca gggcacgcgg ctgtccggcg gcgagcagca gatgctggcc gtcgcgcgca
     2341 tcctgcgcac cggcgcgcgg ctgctgctgc tcgacgagat ctccgaaggg ctcgcgccgg
     2401 tgatcgtgca gaagctcgcc gagatggtcg tcgcgctgcg caagcagggc tacacggtca
     2461 tcatggtcga gcagaacttc cacttcgccg cgccgctggc cgaccgcttc ctcgtgatgg
     2521 agcacggcca ggtgatccag cagttcacgc aggccgagct tccctcgcgc aaggaacgcc
     2581 tgcacgagta cctcggggta tgaaactcgc cccctcgctt cccggatgca tctcgcctga
     2641 accacccccc aagccacgcc agaccaaacc aatggagacc tcacacatgt cgatcaagca
     2701 cacgctcaag tccgtcgcgt tcgcctgtgc cgccgcgctg ggcttcgccg gctctgccca
     2761 cgcccagatc tctggcgacg tgatccgcat cggcttcatc accgacctgt cgggcgtgta
     2821 cgccgacatc gacggcccgg gcggcgtcga ggcgatcaag atggcgatcg ccgacgccgg
     2881 cggccagatc aacggcaaga agatcgagct gctgaccgcc gaccaccaga acaagcccga
     2941 cgtcgccgcg gccaaggcgc gcgagtggat cgacacccag ggcgtcgaca tgatcatcgc
     3001 gggcacgaac tccggcacca acctggcgat ggccaaggtc gcggccgaga agcacaagct
     3061 gctgatctcg gtgggcggcg gcacctccgc gctgaccaac gagcagtgct cgccgaccgt
     3121 cctgcactgg gcctacgaca cgaccgcgct cgccaagggc accggctcgg cggtcaccaa
     3181 ggccggcggc aagacctggt acttcctgca ggccgactac gcgttcggcc aggcgctcga
     3241 gtcggacacc gccaaggtcg tcgaggccaa cggcggcaag gtgctgggcg tcgtcaagca
     3301 cccgctggcg gcgagcgact tctcgtcctt cctgctgcag gcgcaggcga gcaaggcgca
     3361 gatcctcggc ctcgcgaacg cgggcggcga cacgatcaac gcgatcaagg cggccaacga
     3421 gttcggcatc accaagacga tgaagctggc cggcctgctg atgttcatcc aggacgtgca
     3481 ctcgctcggc ctgaagacga cgcagggcat gtacctgacc gacagctggt actggaacaa
     3541 gacgcccgag acgcgcgcct ggggccgccg cttcttcgac gtgatgaaga agatgcccgg
     3601 ctcgctgcac gcgggcgact actcggccgc cgcgaactac ctggccgcgg tcaaggccac
     3661 gggctcggac gacaccgaca aggtgctcgc gaagatgaag gcgaccaagg tcaacgacat
     3721 cttcgccaag gacggctgga tccgcggcga cggcagcatg atccacgaca tgtacctcat
     3781 gcaggtcaag acgccggagg cctcgaagga gccctgggac tactacaacg tggtcgagac
     3841 gatcaagggc gaggccgcgt ggacgacgaa ggccgaaagc aagtgcgccg cctggaaatg
     3901 acggcccccg gccgccgcac gcggcggccg gcccccgagg gggcgcggcc ttgctccgga
     3961 gcggcaggac gcggcggcgg cgagcctgcg ccctgcgctt gatcgcccgc gcgtcgcgcg
     4021 cgaatccgtg agatgcagac aaagccccgg gccgtcgcca cgacggcccg gcgcacatga
     4081 gtggagaggt ggcagatgac cgagatcttc ggcgtcccca tacaagcgct gttcgggcag
     4141 ctgatgctcg gcctggtcaa cggctcgttc tacgcgatcc tcagcctggg cctggcggtg
     4201 atcttcggcc tgctgggagt cgtgaacttc gcgcacggcg cgctgtacat gatcggcgcc
     4261 tacgtggcgt ggttcctgat ggaccgctgg ggcctcggct tctggtgggc gctggcgctc
     4321 gggccgctcg ccgtgggcgt gctgggcgcg gtcatcgagc ggctgctgct caagcgcctg
     4381 tacaagctcg acccgctgta cgggctgctg ctgaccttcg gcctcgcgct cgtcttccag
     4441 ggcgttctgc tgcagtacta cggctcgtcg ggccagtcct acgacgcgcc cgacgcgctg
     4501 tcgggcgcga ccaacctcgg cttcatggtg ctacccaact accgcggctt cgtgatcgtg
     4561 gcgtcgctcg tgatctgcct gggcacctgg ttcctgatcg agcgcacgcg cctgggcgcg
     4621 ctgctgcgcg ccggcaccga gaacccgtcg ctcgtgcagg ccttcggcgt caacgtgccg
     4681 ctcatggtga cgctcaccta cgcgttcggc gcggcgctcg ccggcctggc cggggtgctg
     4741 gcggcgccgg tgatccaggt cacgccgctg atgggcgcca acctcatcat cgtcgtgttc
     4801 gcggtcgtgg tgatcggcgg catgggctcg atcatcggct ccatcgtcac cggcctggtg
     4861 ctgggcctgg tcgagggcct gacgcgcgtg ttctaccccg aggcgtcgaa cgtggtggtg
     4921 ttcgcgatca tggccatcgt gctgatgatc cgccccgccg gcctgttcgg gaaggagaag
     4981 tgatgagcgc cgcccgcccg cccgcaggcg cccgcaccgc agtgcgcagc acggaggttt
     5041 tcgaatgaac tggctcggca acggcacgga ccgctccggc aagctcgtct ggatcgcggc
     5101 gctcgcgatc ctgatcgtcg cgcccttcgt gggcatctac cccatcttcg tcgcggaggc
     5161 gctgtgcttc gcgatcttcg cgatcgcgtt caacctgctg ctcggcttca ccggcctgct
     5221 gtcgttcggc catgcggcct tcttcggcgg cgcggcctac gtgtgcgcct ggctgctgca
     5281 ggacaagcac tggacgcccg agctcgcgct gctgggcggc acggccgcgg cggcggccgc
     5341 gggcctggtg gtcggcgcga tcgcgatccg gcgccagggc atctacttcg cgatggtcac
     5401 gctggctctc gcgcagatgc tgtacttctt cttcctgcag gcgcccttca ccggcggcga
     5461 gaacggcatc caggggatcg cgcgcggacg gctgttcggc ctgatcccgc tcgagcacga
     5521 cctcgcgctg tacttcttcg tgctcgcggt cttcgtcggc gtgttcctgc tcgccctgcg
     5581 catcgtgcac tcgccctacg gccaggtgct caaggcgatc cgcgagaacg agccgcgcgc
     5641 cgtctcgctg ggctacaacg tcgaccgcta caagctgctc gcgttcgtgc tgtcggccgc
     5701 gctggcgggc ctgggcggcg gcctgaagac gctcgtgctg cacctggcga cgctccagga
     5761 cgtgcggtgg gagcagtcgg gcgaggtcat cctgatgacg ctgctgggcg gcctgggcac
     5821 ctacgcggga ccggtggtcg gcgccttcgt cgtcgtcggc ctgcagaact acctgtccga
     5881 ccgcgccggc tcgtggacgg gcgtgatcat cggcgcgatc ttcgtgctgt gcgtggtcgc
     5941 gttccgcaag ggcttcgtcg gcgagctgct cgcgtgggcg aagcggcgcg ccagggcctg
     6001 acgtggcgcg cggcaggacc tggttccgcg cccggaagtt cggctgggga tggggcctgc
     6061 cgaacacctg gcagggctgg ctggtctacg cggcctacgc gctgctgctt gccggcggct
     6121 ggcgcgcgtt cccgctgccg caccatgccg cggccttcgc gatctgggcg gccggactga
     6181 cggcgctgct ggccgcgatc tgctacgcca ccggcgacaa gccgcgctgg cgctgatcag
     6241 aagcgcgtca gcggcacctg cgccgactcg gcgttgtcgg tgaccacgcc gccgcgcagc
     6301 cggcgcgggt actgcagggc caggcccgcg gcggatcccg ggacgtcctc gcagcgcacc
     6361 agccagtgga tggcggtcgt cttctgctcg cgcaggcgcg atccggccac ctcctggatc
     6421 aggtgcgcga gcgagaccgg gcgcccggcg tccgtgccgt ccagcgggat cgcgagctcg
     6481 ccggtgctgc gccggtagac gcccgagggc accttgccgt ccggctggtt ccagcagaaa
     6541 taggccggca gctgctcgcc cgcggtggcg acccagtcgg ggaacatgcc cagctccacc
     6601 atgaaccggt cgaggatggt gcgggccttg tagatctgcg gcgccttcag gcgcgtgaag
     6661 tagaagtgga tctcgacatg gtcgggcacg cgcagcgcct cgtcgcgata gcgcgacccc
     6721 tgtcccgaaa cgacgtagct cttggtcatc cgatcacctt tgggacagca tacccgcccg
     6781 ggtatggtga atccagcctg ccgggggcgg ccgcgtccct agaaacgcag gattgtcgg
//
LOCUS       DASVLN010000848         4789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_205227, whole genome shotgun sequence.
ACCESSION   DASVLN010000848 DASVLN010000000
VERSION     DASVLN010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205227"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..745)
                     /locus_tag="VF453_20000"
     CDS             complement(95..745)
                     /locus_tag="VF453_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690000.1"
                     /translation="MPRTLTAAAVLAAFAALAVAPAHAQTAAHAKKTAKKAPARKSAA
                     PEPTSATNDNLNPGQLTVADRVLTGLAKCELHESVDIEKIPGHAGNFKLTYDHHSYVM
                     VPEETTTGAVRLVDTAGSMVWIQIPRKSMLMNQKAHQRLVDDCEEDEQRIAVGSSSSA
                     SANAPVLAPSVAPPANAGATTTTTTTTTTTTVKLVPPDAQKRGAVAPDAAASGMWK"
     gene            complement(914..1939)
                     /locus_tag="VF453_20005"
     CDS             complement(914..1939)
                     /locus_tag="VF453_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX7690001.1"
                     /translation="MPTAPARHPRDVLFEDGDPTVALPVCDHYAGDEARMRKSLALQA
                     ELGPVFDVTLDAEDGAAVGGELEQARLIGELVQSPANRFDRVGARIVPRGHAAFEGML
                     EILVRAAGQRLAYLMLPKARDAADLDAAIAAIDRVAHAAGVARPIPLHALVETHGALR
                     DVQAFAAHPRIESLSFGLMDFVSAHRGAIPQTAMGAEGQFAHPLVVRAKLEIAAACHG
                     HGKVPAHCVVTEFKDLRLLQHAAERACRQFGYTRMWSIHPDQVRVIVDAFAPTAAEVD
                     LAIEVIHAAEAAGWGPIRHRHGGREHLHDRASYRYFWQVIERAHRTSIGGEPQLPAEV
                     RLRYFGG"
     gene            2091..3656
                     /locus_tag="VF453_20010"
     CDS             2091..3656
                     /locus_tag="VF453_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HEX7690002.1"
                     /translation="MQRRGFLGASVLALFSPATLLATAARAAGLATIGTPERFDYAAL
                     KGQARALAGSPYKPYEGGLPPAVAGLDWDQYQAIRFKPEHALWAGNPELHARAELFHL
                     GLYFKRPVRMYEVADGQARELAYDPALFDYGRSGLASTRLPASLGFAGFRLKTDLAPE
                     TDYAAFLGASYFRATSGTRQYGLSARGLAIDTGLSRPEEFPDFVAYYLERPRAESNTV
                     TVYGLLDSPSVAGAYRFVITPGDTTRMDIDAALYPRKLIERLGIAPCTSMYQTGENDR
                     RMANDWRPELHDSDGLSIHTGSGEWLWRPLRNPPQIRFSAFADRNPRGFGLLQRDRDF
                     DHYQDDGAFYDRRPSLWVEPRGDWGEGAVDLIELPTADETFDNIVAFWNPAKKPEPGS
                     EALLAYTLYWCREAPRQSPLARVVATRTGIGGVVGKPRDHESFRFAIDFAGGDFATLD
                     TRTKVEPVISVTRGRTEVTSARPLDSVHGWRAMFDYVPEPGNEPATLRLFLRANGQPL
                     TETWIYEWDPPRG"
     gene            complement(3832..>4789)
                     /locus_tag="VF453_20015"
     CDS             complement(3832..>4789)
                     /locus_tag="VF453_20015"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7690003.1"
                     /translation="TGAAGQIGYALLFRIASGEMLGKDQPVILQLLEIPDEKAQKALK
                     GVIMELDDCAFPLLAGIEAHSDPMTAFKDTDYALLVGARPRGPGMERADLLAANAQIF
                     TAQGKALDKVASRDVKVLVVGNPANTNAYIAMKSAPSLPRENFTAMLRLDHNRAASQI
                     AAKAGVKVGDIEKLTVWGNHSPTMYADYRFATANGKSLKDAINDQEWNANVFLPTVGK
                     RGAAIIEARGLSSAASAANAAIDHMRDWALGSKGKWVTMGVPSNGEYGIPKDVIFGFP
                     VTTENGKYKIVEGLEIDAFSQERLNKTLDELKGEQDGVKHLL"
BASE COUNT          621 a         1709 c         1741 g          718 t
ORIGIN      
        1 cgtcgcggcc ccctcgtcgc ggcgcatgcc ggaaacgacg cggccgcccg ggggcggccg
       61 caggctgagg ggcgcgctca ggcgcgcgcc gcgatcactt ccacatgccc gacgccgcgg
      121 cgtccggggc caccgcgccg cgcttctgcg cgtccggcgg cacgagcttg acggtggtcg
      181 tcgtggtcgt ggtggtcgtc gtcgtggtgg cgccggcgtt ggccggggga gcgaccgacg
      241 gcgccagcac cggcgcgttg gcgctggccg aggacgacga gccgaccgcg atgcgctgtt
      301 cgtcctcctc gcagtcgtcg accaggcgct ggtgcgcctt ctggttcatc agcatcgact
      361 tgcgcgggat ctggatccac accatcgacc cggcggtgtc gaccaggcgc accgccccgg
      421 tggtggtctc ctcggggacc atgacgtagc tgtggtggtc gtaggtgagc ttgaagttgc
      481 cggcgtggcc cgggatcttc tcgatgtcga cgctctcgtg cagctcgcac ttggcgaggc
      541 cggtgagcac gcggtcggcg accgtcagct ggcccgggtt gaggttgtcg ttggtggcgg
      601 aggtcggctc cggcgcggcg gacttcctgg cgggcgcctt cttggccgtc ttcttggcgt
      661 gggcggcggt ctgcgcgtgg gccggcgcga cggcgagcgc ggcgaaggcg gcaagcacgg
      721 ccgcggcggt cagggtgcgg ggcatgaacg ggtctcctgg gtgctgggag gggtgcggga
      781 agagtgcccg gcattgtcgg ccaatgccgg gccgcgtcag ctcgacaggt gcaacccctt
      841 gtttcgccgc gttccatttg tacgacgtgc atgacccggc tccgggaagg cccggattcc
      901 cgcgcgcgcc gggtcagccg ccgaagtagc gcaggcgcac ctccgcgggc agctgcggct
      961 cgcccccgat cgacgtgcgg tgcgcgcgct cgatcacctg ccagaagtag cggtagctcg
     1021 cgcggtcgtg caggtgctcg cggccgccgt ggcgatggcg gatcgggccc cagcccgccg
     1081 cctcggcggc gtggatgacc tcgatcgcga ggtcgacctc cgccgcggtg ggcgcgaacg
     1141 catcgacgat cacgcgcacc tggtcgggat ggatgctcca catgcgcgtg tagccgaact
     1201 ggcggcaggc gcgctcggcg gcgtgctgca gcaggcgcag gtccttgaac tcggtgacga
     1261 cgcagtgcgc gggcaccttg ccgtggccgt ggcaggcggc ggcgatctcg agcttggcgc
     1321 gcacgacgag cggatgcgcg aactgcccct cggcgcccat cgccgtctgc gggatcgcgc
     1381 cgcggtgcgc ggacacgaag tccatcaggc cgaaggacag cgactcgatg cgcggatgcg
     1441 ccgcgaacgc ctgcacgtcg cgcagcgcac cgtgcgtctc gacgagcgcg tgcagcggga
     1501 tcggacgcgc gacgcccgcc gcgtgcgcga cgcggtcgat ggcggcgatc gcggcgtcga
     1561 ggtcggcggc gtcgcgcgcc ttcggcagca tcagataggc caggcgctgg ccggccgcgc
     1621 gcacgaggat ctcgagcatg ccttcgaacg ccgcgtggcc gcggggcacg atgcgcgcgc
     1681 cgacgcggtc gaagcggttg gccggcgact gcaccagctc gccgatcagg cgcgcctgct
     1741 cgagctcgcc gccgaccgcg gcgccgtcct cggcgtcgag cgtgacgtcg aacaccggcc
     1801 cgagctcggc ctgcagggcg aggctcttgc gcatgcgcgc ctcgtcgccc gcgtagtgat
     1861 cgcagaccgg cagcgcgacg gtcggatcgc cgtcctcgaa caggacgtcg cgcgggtggc
     1921 gggcgggggc ggtgggcatg ggcgttcggc gatcacggtc gggaatcaca cactgtagcg
     1981 ccacggccgc ggtgcgggcc gtccgcgaat caggcgccgt ccgacatctg cgcggccgcg
     2041 cgcgatggac aatcggccgc tgctcaacca cgcgaccggc tgtccccatg atgcaacgac
     2101 gcggcttcct cggcgcctcc gtgctggcgc tgttctcgcc cgccaccctg ctcgccaccg
     2161 cggcccgcgc cgccggcctc gcgacgatcg gcacgcccga gcgcttcgac tacgccgcac
     2221 tcaagggcca ggcgcgcgcg ctcgcgggct cgccgtacaa gccgtatgag ggcggcctgc
     2281 cgccggcggt cgcgggactc gactgggacc agtaccaggc gatccgcttc aagcccgagc
     2341 atgcgctgtg ggccggcaac ccggagctgc atgcgcgcgc cgagctgttc cacctgggcc
     2401 tgtacttcaa gcgcccggtg cgcatgtacg aggtcgccga cggccaggcg cgagagctcg
     2461 cctacgaccc ggcgctgttc gactacggca ggagcggcct cgcgtcgacc aggctgcccg
     2521 cgagcctcgg cttcgcgggc ttccggctga agaccgacct cgcgccggag accgactacg
     2581 cggccttcct cggcgcgagc tacttccgcg cgaccagcgg cacgcgccag tacgggctgt
     2641 ccgcgcgcgg cctggcgatc gacaccgggc tgtcgcggcc cgaggagttc ccggacttcg
     2701 tcgcctacta cctcgagcgc ccgcgcgccg agtccaacac ggtcaccgtc tacggcctgc
     2761 tcgactcgcc cagcgtcgcc ggcgcctatc gcttcgtcat cacgcccggc gacaccacgc
     2821 gcatggacat cgacgccgca ctctacccgc gcaagctcat cgagcgcctc ggcatcgcgc
     2881 cgtgcacgag catgtaccag accggcgaga acgaccgccg catggccaac gactggcgcc
     2941 cggagctgca cgactccgac ggcctgtcca tccacaccgg cagcggcgaa tggctgtggc
     3001 gtccgctgcg caacccgccg cagatccgct tcagcgcgtt cgcggatcgc aacccgcgcg
     3061 gcttcggcct gctgcagcgc gaccgcgact tcgaccacta ccaggacgac ggcgcgttct
     3121 acgaccgccg ccccagcctc tgggtcgagc cgcgcggcga ctggggcgaa ggcgcggtcg
     3181 acctgatcga gctgcccacc gccgacgaga ccttcgacaa catcgtcgcc ttctggaacc
     3241 cggcgaagaa gcccgagccc ggctccgagg cgctgctggc ctacacgctg tactggtgcc
     3301 gcgaggcgcc gcggcagtcg ccgctcgccc gcgtcgtcgc cacgcgcacg ggcatcggcg
     3361 gcgtggtcgg caagccgcgc gaccacgagt ccttccgctt cgcgatcgac ttcgccggcg
     3421 gcgacttcgc gacgctggac acgcgcacca aggtcgaacc cgtcatctcc gtgacgcgcg
     3481 gccgcaccga ggtcacctcg gcccggccgc tcgattcggt gcacggctgg cgcgcgatgt
     3541 tcgactacgt ccccgagccc ggcaacgagc cggcgacgct gcggctgttc ctgcgcgcca
     3601 acgggcagcc gttgacggag acgtggatct acgagtggga tccgccgcgg ggctgaccac
     3661 cgcgctcgcg agtttcggcc actgcccctg cgcacccgag gcccgttgga cttccagcgc
     3721 ccgcgagcga atggctgcca gcccgagaag cctcgctcaa tggaaaaggc cgcccgaagg
     3781 cggcctctcg acggttgcgg cgcgagccct cgggcccgcg cggccggcgg atcacagcag
     3841 gtgcttgacg ccgtcctgct cgcccttgag ctcgtcgagc gtcttgttca ggcgctcctg
     3901 cgagaaggcg tcgatctcga ggccctcgac gatcttgtac ttgccgttct cggtcgtgac
     3961 cgggaagccg aagatgacgt ccttgggaat gccgtactcg ccgttggagg ggacgcccat
     4021 cgtgacccac ttgcccttgg agcccagggc ccagtcgcgc atgtggtcga tggcggcgtt
     4081 ggcggccgag gcggccgacg acaggccgcg cgcctcgatg atggccgcgc cgcgcttgcc
     4141 gaccgtcggc aggaacacgt tggcgttcca ttcctggtcg ttgatcgcgt ccttcaggct
     4201 cttgccgttg gcggtcgcga agcggtagtc ggcgtacatc gtgggcgagt ggttgcccca
     4261 gacggtcagc ttctcgatgt cgccgacctt gacgccggcc ttggccgcga tctggctggc
     4321 ggcgcggttg tggtcgaggc gcagcatggc ggtgaagttc tcgcgcggca ggctcggcgc
     4381 gctcttcatg gcgatgtagg cgttggtgtt ggcggggttg ccgacgacca gtaccttgac
     4441 gtcgcgcgag gcgaccttgt cgagcgcctt gccctgcgcc gtgaagatct gcgcgttggc
     4501 ggccagcagg tccgcgcgct ccatgccggg gccgcgcgga cgcgcgccga ccagcagggc
     4561 gtagtccgtg tccttgaacg cggtcatcgg gtcgctgtgg gcctcgatgc cggcgagcag
     4621 cgggaacgcg cagtcgtcga gctccatgat cacgcccttg agcgccttct gggccttctc
     4681 gtcggggatc tccagcagct ggaggatgac cggctggtcc ttgcccagca tctcgcccga
     4741 ggcgatgcgg aacagcaggg cgtagccgat ctggccggcg gcgccggtg
//
LOCUS       DASVLN010000849         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_205319, whole genome shotgun sequence.
ACCESSION   DASVLN010000849 DASVLN010000000
VERSION     DASVLN010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205319"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VF453_20020"
     CDS             complement(<1..447)
                     /locus_tag="VF453_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HEX7690004.1"
                     /translation="MPLTTLDIPTGDAPGAAIIVLHGLGASSQDFVPFVEEIDLAPVG
                     PVRWVFPDAPVRPVTINGGMRMPAWYDILGLDFSVRGEDERGLRASAAEVQALIDREV
                     ARGIPAGRIVLGGFSQGCAMTLLAGLRAPQRLAGLLGMSGYLPLAAT"
     gene            complement(534..944)
                     /locus_tag="VF453_20025"
     CDS             complement(534..944)
                     /locus_tag="VF453_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690005.1"
                     /translation="MTRHPSRLPFAAPSHHALMIGLQVAVALIVACVLTTAAAAAEPV
                     TSEQFAPGWQQKSQALFMRGPMRDGRDAWELPDRLPRGHYVLVGKEDGRIVPIPGSAF
                     DIDTTTGFAKRTMFVRGSFTEVMAVPADMVPQAR"
     gene            complement(1167..2459)
                     /locus_tag="VF453_20030"
     CDS             complement(1167..2459)
                     /locus_tag="VF453_20030"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7690006.1"
                     /translation="MTVRVLTGITTTGTPHLGNYVGAIRPAIAASRAPGVESFLFLAD
                     YHALVKCDDPARVERSRLALAATWLACGLDPERVTFYRQSDIPEIPELTWLLTCVTAK
                     GMMNRAHAYKAAVDANAGGDPDDGISMGLYCYPILMAADILMFNATHVPVGKDQVQHI
                     EMARDVAQRFNHLFGTDGARFVLPEAVTEEATELLPGLDGRKMSKSYDNVVPLFEGGL
                     AALQAAIARVVTDSKLPGEPKEPEGTPLVQLFDAFAGEDERAAFRAELRAGLGWGDAK
                     KRLLATVDRELAPQRARYDEFMARPELIEEQLQVGARKARAIATPFLAELRHAVGLRR
                     FHALASTAPAAAKAAGAAMPVFKQYREADGRFYFKLVGAGDALLLQSEGFDQGRDAGQ
                     WVARLKKEGAAALAGAPVRLEAEEAAVREALARFRDAD"
     gene            complement(2560..3054)
                     /locus_tag="VF453_20035"
     CDS             complement(2560..3054)
                     /locus_tag="VF453_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690007.1"
                     /translation="MSLVRPVLLALAAAVLAPAVSAAPKPAQASAPSAFASREQLRRC
                     LAEEDELKARLDAIQARHAEHERRLSELEAENDRIVEVQRQLDTTSDVAVNAFNLLVS
                     DHNVHARQLNEAAAASRAESEAYNAAILQHNRDCARLVYRVDDLQAVTKERRAAAAAA
                     SAAK"
BASE COUNT          410 a         1112 c         1243 g          409 t
ORIGIN      
        1 ggtcgcggcc agcggcaggt agccggacat gcccagcagc cccgcgagcc gctgcggcgc
       61 gcgcaggccc gccagcagcg tcatcgcgca gccctgcgag aagccgccca gcacgatgcg
      121 cccggcggga atgccgcgcg cgacctcgcg gtcgatcagc gcctgcacct cggcggcgga
      181 cgcgcgcagg ccgcgctcgt cctcgccgcg gaccgagaag tccagcccga ggatgtcgta
      241 ccacgccggc atgcgcatgc cgccgttgat cgtcacgggc cgcacgggcg cgtcggggaa
      301 gacccagcgc accgggccga ccggcgccag gtcgatctcc tcgacgaagg gcacgaagtc
      361 ctgcgagctg gcgcccaggc cgtgcaggac gatgatggcg gcgcccgggg cgtcgccggt
      421 ggggatgtcg agggtcgtga gcggcatggc cgggagcata acgtcccccg ctcgtgcccg
      481 cgccaggccc ggcaggcggc acggaaacgg ggcggccccc ctcgagccgg acttcagcgg
      541 gcctgcggca ccatgtcggc cggcaccgcc atcacctccg tgaaactgcc gcgcacgaac
      601 atcgtgcgct tggcgaagcc ggtggtggtg tcgatgtcga aggccgagcc cgggatcggg
      661 acgatccggc cgtcctcctt gccaacgagc acgtagtggc cgcgcggcag gcggtcgggc
      721 agttcccagg cgtcgcggcc gtcgcgcatc gggccgcgca tgaacagcgc ctgcgacttc
      781 tgctgccagc cgggcgcgaa ctgctcgctg gtgacaggct cggccgccgc ggcggccgtc
      841 gtgagcacgc aggcgacgat cagtgcgacg gcgacctgca gcccgatcat gagcgcatgg
      901 tgggaaggag cggcgaacgg gaggcgggag ggatggcgtg tcatggcggt cctcgctggg
      961 ctggcgttcc acccgccacg gaagaaggcc ctccgcggcg gcccgggcgc ccgcgaaacg
     1021 cggcaagccg cattcccctg cgggcggccc gatgccgcga tggtgcgccg ggacgggccc
     1081 ggccggcaag ggcggcgacg cgactttacg cgttcgtcag gtccggacgc tcgccggaag
     1141 gacgccggcg accgggtgcc cggccgtcag tccgcgtccc ggaagcgcgc gagcgcctcg
     1201 cgcacggcag cctcttcggc ctccaggcgc accggcgccc ccgccagcgc cgcggcgccc
     1261 tccttcttca gccgcgcgac ccactggccg gcgtcgcggc cctggtcgaa gccctcgctc
     1321 tgcagcagca gggcgtcccc ggcaccgacg agcttgaagt agaagcggcc gtccgcctcg
     1381 cggtactgct tgaagaccgg catcgcggcg ccggccgcct tggccgcggc cggcgcggtc
     1441 gatgccagcg cgtggaagcg ccgcagcccg accgcgtggc gcagctcggc gaggaagggc
     1501 gtcgcgatcg cgcgtgcctt gcgggcgccg acctgcagct gctcctcgat cagctccgga
     1561 cgggccatga actcgtcgta gcgcgcgcgc tgcggtgcga gctcgcggtc gaccgtggcg
     1621 agcagccgct tcttggcgtc gccccagccg aggccggcgc gcagctcggc gcggaaggcg
     1681 gcgcgctcgt cctcgcccgc gaaggcatcg aagagctgga ccagcggcgt gccttcgggt
     1741 tccttgggct cgccgggcag cttcgagtcg gtcaccacgc gcgcgatcgc cgcctgcagc
     1801 gccgccagcc cgccctcgaa cagcggcacg acgttgtcgt agctcttgga catcttgcgg
     1861 ccgtccaggc cgggcagcag ctcggtggct tcctcggtga ccgcctcggg cagcacgaag
     1921 cgcgcgccgt cggtgccgaa gaggtggttg aagcgctgcg ccacgtcgcg cgccatctcg
     1981 atgtgctgca cctggtcctt gccgaccggc acgtgcgtgg cgttgaacat caggatgtcg
     2041 gcggccatca ggatcgggta gcagtacagg cccatcgaga tgccgtcgtc cgggtcgccg
     2101 ccggcgttgg cgtcgacggc cgccttgtag gcatgggcgc ggttcatcat gcccttggcg
     2161 gtgacgcagg tgagcagcca ggtgagctcc gggatctcgg ggatgtcgct ctggcggtag
     2221 aacgtgacgc gctcgggatc gaggccgcag gcgagccagg tcgcggcgag cgcgaggcgc
     2281 gagcgctcga cgcgcgccgg gtcgtcgcac ttgaccagcg cgtggtagtc ggcgaggaac
     2341 aggaagctct cgacgccggg cgcccggctc gcggcgatgg cggggcggat cgcgccgacg
     2401 tagttgccca ggtgcggggt gccggtggtg gtgatgccgg tgaggacgcg gacggtcatg
     2461 gcttgcggac gggctgcggg gatcgggaac ggcgcggcgg ccgcgcatgg agcggacgac
     2521 tgtaacgcac gggggccggc ggcacgcgcg ggcgcggcgt cacttcgcgg cggatgcggc
     2581 ggcagcggcg gcgcggcgct ccttcgtcac ggcctgcagg tcgtcgacgc ggtagacgag
     2641 gcgcgcgcag tcgcggttgt gctgcaggat ggccgcgttg taggcctcgg actcggcgcg
     2701 gctcgcggcg gccgcctcgt tgagctgcct ggcgtgtacg ttgtggtcgc tcacgagcag
     2761 gttgaacgcg ttgaccgcga cgtcgctcgt cgtgtcgagc tggcgctgca cctcgacgat
     2821 cctgtcgttc tccgcctcca gctcggacag gcgccgctcg tgctccgcgt ggcgcgcctg
     2881 gatggcgtcc aggcgggcct tcagctcgtc ctcctcggcg aggcaccggc gcagctgctc
     2941 gcgcgaggcg aacgcggatg gcgcggacgc ctgcgcgggc ttgggcgccg cggagacggc
     3001 gggggcgagc accgcggccg cgagcgcgag gaggacggga cggacgaggg acatggcgtg
     3061 gtcggatgga gtcgtggacg cgcggacgcg ccgcgccatt gtcgggcagg ggcgccggcc
     3121 acggcacggg atccgcccca gggatcgccc cgccccgcgc gcggcggcaa aacg
//
LOCUS       DASVLN010000850         4438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_529794, whole genome shotgun sequence.
ACCESSION   DASVLN010000850 DASVLN010000000
VERSION     DASVLN010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529794"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VF453_20040"
     CDS             <1..765
                     /locus_tag="VF453_20040"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7690008.1"
                     /translation="AGAASSPQEPSEEALEARAAALYRDRIDDARAHGLLAPPDDPQR
                     DRLDYIAHRLEAFAPAWNPRAARWRWEVELVRSEEVDADCLPGGKIVVQSGLLARLAP
                     SDDELAMVIGHEMGHALREHARTRLGAQRATSWLISLAAQFFGWSDVGRSVAGAGGNL
                     LALKFSRDEETEADLVGLELAARAGYDPEAAVALWRKMGALARGGPPAWLSDHPSSPE
                     RLQVLSRDVARVRGLYREAARPDRRFAPPAAAPSLP"
     gene            966..1262
                     /locus_tag="VF453_20045"
     CDS             966..1262
                     /locus_tag="VF453_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX7690009.1"
                     /translation="MTSQHDQNRQDAQHSQSAAGQTGQQGAGRPGGRGFAGMDRERQR
                     EIASQGGRAAHASGNAHQFTSEEARAAGSKRHANAAARAAAAARGQGGSDQSLS"
     gene            1501..1893
                     /locus_tag="VF453_20050"
     CDS             1501..1893
                     /locus_tag="VF453_20050"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEX7690010.1"
                     /translation="MDIALIAHDAKKDLMVVLARDFAPFLSRCHLMATGTTGGRLVAE
                     VGLEVERLLSGPLGGDLQIGARLAEGRVDAVIFLRDPMTPQPHEPDINALVRACDVHD
                     VVCATNESTARHVLRALAEELEESLQKR"
     gene            complement(1881..2567)
                     /locus_tag="VF453_20055"
     CDS             complement(1881..2567)
                     /locus_tag="VF453_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690011.1"
                     /translation="MSVRAPIALAALPLVAAAVATAPARAVEPYVATGLPYALAGLAQ
                     PLGSAFAVRADFGTIGGHAYSGSTSDNDFRGKLHYDRIALVGDWFAFGGGFRLTGGLT
                     LNQAKATMRASPHDGKITIGGVAYDAPSSLYYVQSDLSFPHAMPYLGIGWGHHGDDTP
                     GFAFNVDLGAGIGKARATPLAPSPALASEIALTSGGAADLASENRQFQDEVGKFKAIP
                     QATLGIGYRF"
     gene            2773..4368
                     /locus_tag="VF453_20060"
     CDS             2773..4368
                     /locus_tag="VF453_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX7690012.1"
                     /translation="MALTPRPARSARPVRSALPGLLLAGLAACGGGADNLSGTESPPP
                     QSGYVDGNTYSAMRGSSLSTPNEATSATHHTLTIDGTPYAYTATAGHLTAKDLGSGAA
                     EASFFYVAYTVDGAPAATRPVTFFFNGGPGSSTVWLHLGSWAPKRLVTGVPATTQAAP
                     FPYVDSPDSLIDTSDLVFVDAVGTGLSEAIAPNTNQTYWSVDADAQVFRDFVIRWLAA
                     NGRTASPLFVYGESYGTVRAPLLARYLETAGTPVKGVVLQSSILDYNSNCETTVDFLG
                     HPTVDCSGLLPTFGAVGAFWSKVTPPPTDLEAFLTQLRGYADGVYSPQVVQWLGAGGT
                     GGPPAGADVNALQADTGLAASYWTGQFDVDYDDYRHNLIPGQVLGVYDGRMAAAKGSA
                     LAAGDDPSNTFIAQPFEQAITSVLASDLAFTTPGSYVEESDAIDYWDFSHGGRRLPDV
                     VPDLAAAMTLDPGLKVLSIGGEHDLITPFHQTERDLARLAAGAPVTSHFYAGGHMVYL
                     NDPSRAAIKADLKAFYAAAAGAK"
BASE COUNT          619 a         1673 c         1568 g          578 t
ORIGIN      
        1 gccggcgccg cctcctcgcc ccaggagccc tccgaggagg cgctggaagc ccgcgccgcc
       61 gccctctacc gcgaccgcat cgacgacgcc cgggcgcacg gcctgctggc gccgccggac
      121 gatccgcagc gcgaccgcct cgactacatc gcccaccgcc tggaagcctt cgcgccggcg
      181 tggaacccgc gcgccgcccg gtggcgctgg gaagtcgaac tggtgcgctc ggaggaggtc
      241 gacgccgatt gcctgccggg cggcaagatc gtggtgcagt cggggctcct ggcgcgcctc
      301 gcgccgtcgg acgacgagct cgcgatggtg atcggccacg agatgggcca cgcgctgcga
      361 gagcatgccc gcacgcgcct gggcgcgcag cgcgcgacct catggctgat ctcgctggcg
      421 gcgcagttct tcggctggag cgacgtcggg cgctcggtgg cgggcgcggg cggcaacctg
      481 ctggcgctga agttcagccg cgacgaggag accgaggccg acctcgtggg cctcgagctc
      541 gcggcgcgcg ccggctacga ccccgaggcc gccgtcgcgc tctggcgcaa gatgggcgcg
      601 ctcgcccgcg gcgggccgcc ggcgtggctg tccgaccatc cctcgagccc cgagcgcctc
      661 caggtgcttt cacgcgatgt tgcacgcgtg cgcggtcttt acagggaggc ggcccggccc
      721 gatcggcgtt tcgcgccccc ggccgccgcg ccgtccctgc cctgaccgcg cccccattcg
      781 ggttgcgcac cccctaagaa cgtggggatg cgctcgcgcc gttcctggtc gacagttccc
      841 tttcgcgggt ggggaggtat gtgcgttgcc cctgctcacc cgcgatccgg ggagccctga
      901 gcccgcggat cggaatcaca tcccgctgca tggggcggcg gggcaaacct ttcggagtac
      961 cagcgatgac ttctcaacac gatcagaacc gccaggacgc gcagcacagc cagtccgctg
     1021 ccggccagac gggccagcag ggcgcgggcc gtccgggcgg ccgtggcttc gccggcatgg
     1081 atcgcgagcg ccagcgcgag atcgcgagcc agggcggccg cgccgcccat gcgagcggca
     1141 acgcgcacca gttcacctcc gaggaagccc gtgccgccgg ctccaagcgc catgccaacg
     1201 ccgccgctcg cgccgcggcg gccgcccgcg ggcagggtgg cagcgaccag tccctgagct
     1261 gacccccgcc gccgctggca tcgcgggggc ggcaaacgcg gccgccgggc ccttccggta
     1321 tggccgcgtt tgccgcaaac gctacagcaa cagtggccgg gctggcagcc ggctcgtgcg
     1381 ggccctcgtc agggggcgtc gtgcgatccg caacaggctg cggcccgatc gcaccatcgc
     1441 ggggcacgcg cccgcgaaaa tgagcgaacg atgtaacgcc acgtccgtat catgcggacg
     1501 atggacatcg ccctcatcgc gcacgacgcc aagaaggacc tgatggtcgt cctcgcgcgc
     1561 gatttcgccc ccttcctctc acgttgccac ctcatggcca ccggcacgac gggcggccgc
     1621 ctcgtggccg aggtcggcct ggaggtggag cgcctcctga gcggaccgct cgggggcgac
     1681 ctgcagatcg gcgcccgcct ggccgagggc cgggtcgacg ccgtgatctt cctgcgcgac
     1741 ccgatgacgc cgcagccgca cgagcccgac atcaacgcgc tcgtgcgcgc ctgcgacgtg
     1801 cacgacgtcg tctgcgccac caacgagtcc acggcgcgcc atgtgctgcg cgcgctggcc
     1861 gaggaactcg aggagtcgct tcagaagcgg tagccgattc ccagcgtcgc ctgcgggatc
     1921 gccttgaact tgccgacctc gtcctggaac tgccggttct cggacgcgag gtcggccgcg
     1981 ccgccactgg tcagcgcgat ctcgctcgcg agcgccggcg agggcgccag cggcgtcgcg
     2041 cgggccttgc cgatgccggc gccgaggtcg acattgaacg cgaagccggg cgtgtcgtcg
     2101 ccgtggtggc cccagccgat gcccaggtac ggcatcgcgt gcgggaagga caggtccgac
     2161 tgcacgtagt acagcgacga cggcgcgtcg taggcgacgc cgccgatcgt gatcttgccg
     2221 tcgtgcggcg aggcgcgcat cgtcgccttg gcctggttga gggtcagccc gccggtcagg
     2281 cggaagccgc cgccgaaggc gaaccagtcg ccgaccaggg cgatccggtc gtagtggagc
     2341 ttgccgcgga agtcgttgtc ggacgtgctg ccgctgtagg cgtggccgcc gatcgtgccg
     2401 aaatcggcgc gcacggcgaa cgcgctgccc agcggctggg cgaggccggc cagcgcgtag
     2461 ggcaggccgg tggcgacgta gggctccacg gcgcgcgcag gcgcggtggc gacggccgcg
     2521 gcgaccagcg gcagggcggc gagggcgatc ggggcgcgga cggacatggg aacgttcctg
     2581 ggtgggtgga tacggacgcg aggcgtgcgc agacctgcaa gactagcgtt gcaggcgccg
     2641 ttcgatcgcc ggagcagcgg caagcggacg gcccgattcc ggcgtccgga atgccccaca
     2701 atccgcccca cccgaccccg tgaccacctg accccccgac ccgcggccgg cgccgcgggc
     2761 tgaaaggcac cgatggccct gactccacgc cccgcgcgat ccgcgagacc cgtccggtcc
     2821 gcgctgcccg gcctgctgct cgccggcctc gcggcgtgcg gcggcggcgc cgacaacctt
     2881 tccggcacgg aaagcccgcc gccgcaatcc ggctatgtcg acggcaacac ctactcggcc
     2941 atgcgcggca gctcgctgtc cacgcccaac gaggcaacga gcgcgaccca ccacaccctg
     3001 acgatcgacg gcacgcccta cgcctacacg gccaccgcgg ggcatttgac ggccaaggac
     3061 ctgggctccg gcgccgcgga ggcctcgttc ttctacgtcg cctacaccgt cgacggcgcg
     3121 cccgcggcga cccggccggt gaccttcttc ttcaacggcg gcccgggctc gtccaccgtc
     3181 tggctgcacc tgggctcgtg ggcgcccaag cgcctggtca cgggcgtgcc ggcgacgacc
     3241 caggcggcac cattccccta cgtcgacagc cccgacagcc tgatcgacac cagcgacctc
     3301 gtcttcgtcg acgcggtggg caccggcctg tccgaggcga tcgcgcccaa caccaaccag
     3361 acctactgga gcgtcgacgc ggacgcgcag gtcttccgcg acttcgtgat ccgctggctg
     3421 gcggccaacg gccgcacggc gtcgccgctg ttcgtctacg gcgagtccta cggcaccgtg
     3481 cgcgcgccgc tgctggcgcg ctacctggag accgcgggca cgccggtcaa gggcgtggtc
     3541 ctgcagtcct ccatcctcga ctacaacagc aactgcgaga cgaccgtcga cttcctcggc
     3601 caccccaccg tggactgcag cggcctgctg ccgacgttcg gggcggtcgg cgccttctgg
     3661 agcaaggtga cgccgccgcc gaccgacctc gaggccttcc tgacccagct gcgcggctat
     3721 gccgacggcg tctacagccc gcaggtcgtg cagtggctgg gcgcgggcgg caccggcggg
     3781 ccgccggccg gcgcggacgt gaacgccctg caggccgaca ccggcctcgc cgcgtcgtac
     3841 tggacgggcc agttcgacgt cgactacgac gactaccgcc acaacctgat ccccggtcag
     3901 gtcctgggcg tgtacgacgg gcgcatggcc gcggccaagg gctcggcgct ggcggccggc
     3961 gacgatccgt cgaacacctt catcgcgcag cccttcgagc aggcgatcac gagcgtgctg
     4021 gcgagcgacc tcgcgttcac gaccccgggc agctacgtgg aggagagcga cgcgatcgac
     4081 tactgggact tctcgcacgg cggccgcaga ctgcccgacg tcgtgcccga cctcgctgcc
     4141 gccatgacgc tcgaccccgg cctgaaggtg ctgtccatcg gcggcgagca cgacctgatc
     4201 acgccgttcc accagaccga gcgcgacctc gcgcggctgg ccgccggcgc gccggtcacc
     4261 agccatttct acgcaggagg ccacatggtc tacctcaacg acccgtcgcg cgcggcgatc
     4321 aaggcggacc tgaaggcgtt ctacgccgcg gccgcggggg cgaagtgatg cgcgcccgtc
     4381 ccgtcctcgc gatcttcgcg gcgctggccg ccgtcggcgc cggcgcgcag ccgccggc
//
LOCUS       DASVLN010000851         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_420464, whole genome shotgun sequence.
ACCESSION   DASVLN010000851 DASVLN010000000
VERSION     DASVLN010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420464"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1569)
                     /locus_tag="VF453_20065"
     CDS             complement(70..1569)
                     /locus_tag="VF453_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX7690013.1"
                     /translation="MDFTHINGTAFGVFVFFFALVTVIGFVASRWQRGTAHKGKHLDE
                     WGLGGRNFGAWITWFLVGGDFYTAYTVIAVPALVYAMGAYGFFALPYTIVVYPIVFLI
                     MPKLWKEAQGAGHVTAADVVYGRYGSRALELAIAVTGIVATMPYIALQLVGMEAVIKA
                     LGLSGELPLIAAFVILALYTYAAGLRAPALIAFVKDIMIYIVVIAAVVIVPMKVGGYG
                     SVFSAAHDAFAAKGGATGTTLHPGQMMPFATLALGSAMAAFMYPHTLTGIFAARSAST
                     IRKNAIYLPAYTVLLGLIALLGFMAYAAKISVKSNNDVVPALFNALFPSWFAGFAFAA
                     IAIGALVPAAVMSIGASNLFTRNLWKPYVNAGITPAQEEGVAKVVSLVVKVGALLCIL
                     YLPTKFALDLQLLGGVWILQTFPAVVSGLFSKRLRAPALLAGWLAGIVYGTWTAFADG
                     TKPVHSLVIGGDKYTFYTGLLALVLNFVVAFAVQAVLRTPEPALVARKA"
     gene            complement(1580..1753)
                     /locus_tag="VF453_20070"
     CDS             complement(1580..1753)
                     /locus_tag="VF453_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX7690014.1"
                     /translation="MRLLLLLPFVGLLAVPLYNSETPEFAGFPFFYWYLFAWVPVTSL
                     LIWIVNRGEPSDD"
     gene            complement(1969..>2989)
                     /locus_tag="VF453_20075"
     CDS             complement(1969..>2989)
                     /locus_tag="VF453_20075"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7690015.1"
                     /translation="GGVMGRPLELVVKDDRGDPDTGRAAAQALVADPGIAATVGYCNT
                     GVALKALDVFEAARRVLVIPCAQGTELTRRVPAAESMVFRVAPADVLTAEFLVGEIVD
                     RRHLSRIAILADRTGYGDSGLADLGAELKKRGLAPAYVGRFAPDATSLHDELEDARAA
                     GAQALVVYTVGPGEAVAVRGRAAMHWDVPYFAPWTLSFRSVLDNAGAQALEGTMMTQS
                     LIQDAANVARTAFIAAFARRNQHRPIGSLMAAAQAYDSVQLVVRALFETGGDASGGAL
                     KNALEDPREPYRGVVTTYDRPFSMHDHEAFSRNMIWLGVWRGGEIRYFYPEDARLSGA
                     VRRKQ"
BASE COUNT          484 a         1044 c         1070 g          391 t
ORIGIN      
        1 ggcggacggg ctcgcgcgcc gtccgccgcc gcgacgggac ccggcgcgcc gggcccgtcg
       61 ttcacgccgt caggccttgc gggcgaccag cgcgggctcc ggcgtgcgca gcaccgcctg
      121 caccgcgaac gcgacgacga agttcagcac cagggccagc aggccggtgt agaacgtgta
      181 cttgtcgccg ccgatcacca gcgagtgcac cggcttcgtg ccgtcggcga aggccgtcca
      241 cgtgccgtag acgatgcccg ccagccagcc cgccagcagg gccggcgcgc gcaggcgctt
      301 cgagaacagg cccgagacga ccgccgggaa ggtctgcagg atccagacgc cgcccagcag
      361 ctgcaggtcg agcgcgaact tcgtcggcag gtacaggatg cacagcagcg cgccgacctt
      421 gaccaccagc gacacgacct tcgcgacgcc ctcttcctgc gccggcgtga tgccggcgtt
      481 gacgtagggc ttccacaggt tgcgggtgaa caggttggac gcgccgatcg acatcaccgc
      541 cgccggcacc agcgcgccga tcgcgatggc cgcgaacgcg aagcccgcga accacgacgg
      601 gaacagcgcg ttgaacagcg ccggcacgac gtcgttgttg ctcttgacgc tgatcttggc
      661 ggcataggcc atgaagccca gcagcgcgat caggcccagc agcacggtgt aggcgggcag
      721 gtagatcgcg ttcttgcgga tcgtggacgc gctcctggcc gcgaagatgc cggtcagcgt
      781 gtgcgggtac atgaacgccg ccatcgccga gccgagcgcc agcgtcgcga acggcatcat
      841 ctggcccgga tgcagcgtcg tgcccgtggc gccgcccttg gccgcgaacg cgtcgtgcgc
      901 ggccgagaac acgctgccgt agccgcccac cttcatcggc acgatgacca ccgccgcgat
      961 caccacgatg tagatcatga tgtccttgac gaaggcgatc agcgcgggcg cgcgcaggcc
     1021 ggcggcgtag gtgtagagcg cgaggatcac gaaggccgcg atcagcggca gctcgcccga
     1081 gaggccgagc gccttgatga ccgcctccat gccgaccagc tgcagcgcga tgtagggcat
     1141 ggtcgcgacg atgccggtga cggcgatcgc cagctccagc gcgcgcgagc cgtagcggcc
     1201 gtagacgacg tccgccgccg tcacgtggcc ggcgccctgc gcctccttcc acagcttggg
     1261 catgatgagg aacacgatcg gatagacgac gatcgtgtag ggcagcgcga agaagccgta
     1321 ggcgcccatc gcgtagacca gcgccggcac ggcgatcacg gtgtaggcgg tgtagaagtc
     1381 gccgccgacg aggaaccagg tgatccaggc gccgaagttg cgcccgccca ggccccactc
     1441 gtcgaggtgc ttgcccttgt gcgcggtgcc gcgctgccag cgcgaggcca cgaagccgat
     1501 caccgtgacc agcgcgaaga agaagacgaa cacgccgaag gcggtgccgt tgatgtgggt
     1561 gaagtccatg ggatgctcct cagtcgtcgc tgggctcgcc gcgattgacg atccagatca
     1621 gcagcgaggt gacgggcacc cacgcgaaca ggtaccaata gaagaacggg aaaccggcga
     1681 actcgggcgt ctcgctgttg tacagcggca ccgcgagcag gccgacgaac ggcagcagca
     1741 acagcaggcg catggcttgt ctcctgggtg aacggcggac gtggtgcccg ctctgacggt
     1801 agtgtcgacg gtggtgcccc gcagggcatc cgtcaagttg cgcaacggtg cgcgcgagat
     1861 cacctaatgg ttaaccccgg atcgatggcg cctggactgc cggcaagcgg cccgccggcc
     1921 gcccccggcg gagcgtcccg gtcgggaacc ctctgccgca tgagcggctc actgcttgcg
     1981 gcgcacggcg cccgacaggc gcgcgtcctc gggatagaag tagcggatct cgccgccgcg
     2041 ccacacgccc agccagatca tgttgcgcga gaaggcctcg tggtcgtgca tcgagaacgg
     2101 gcggtcgtag gtcgtgacga cgccgcggta gggctcgcgc ggatcctcga gcgcgttctt
     2161 cagcgcgccg ccgctcgcgt cgccgccggt ctcgaacagc gcgcgcacga ccagctgcac
     2221 gctgtcgtaa gcctgcgccg cggccatcag cgagccgatg gggcggtgct ggttgcggcg
     2281 cgcgaatgcc gcgatgaagg ccgtgcgcgc gacgttggcc gcgtcctgga tcaggctctg
     2341 cgtcatcatc gtgccctcga gcgcctgggc gccggcgttg tccaggacgc tgcggaacga
     2401 cagcgtccac ggcgcgaagt acggcacgtc ccaatgcatc gccgcgcgcc cgcgcacggc
     2461 cacggcctcg ccgggcccga ccgtgtagac gacgagggcc tgcgcgccgg cggcgcgggc
     2521 gtcttcgagc tcgtcgtgca gcgacgtggc gtcgggcgcg aagcggccca cgtaggccgg
     2581 ggcgaggccg cgcttcttca gctcggcgcc caagtcggcg aggccgctgt cgccgtagcc
     2641 ggtgcggtcg gccaggatcg cgatgcgcga gagatggcgg cggtcgacga tctcgccgac
     2701 caggaactcc gcggtcagca cgtcggcggg cgcgacgcgg aacaccatgc tctcggcggc
     2761 cggcacgcgc cgcgtgagct cggtgccctg cgcgcacggg atgacgagca cgcgccgggc
     2821 ggcctcgaac acgtccagcg ccttcagcgc gacgcccgtg ttgcagtagc ccaccgtcgc
     2881 cgcgatgccg ggatcggcca ccagcgcctg cgccgcagcg cgccccgtgt cggggtcgcc
     2941 gcggtcgtcc ttcacgacga gttccagcgg ccggcccatc acgccgccg
//
LOCUS       DASVLN010000852         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_366600, whole genome shotgun sequence.
ACCESSION   DASVLN010000852 DASVLN010000000
VERSION     DASVLN010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366600"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..768)
                     /locus_tag="VF453_20080"
     CDS             complement(10..768)
                     /locus_tag="VF453_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690016.1"
                     /translation="MRHVILRRPTRCLPALLAAFAAPGFAAHPLQTEDTGTQGAGNVE
                     IENGFQRARVGGVTTFTYQPQVSVGLATTVDAIVQPSWVSQHDPAQGTSAGVGDTNLD
                     AKWRFWGADPWSLGVRAGIEAPTARHGLGLPHGRIAEHALAVATWDASPTTVHANLGF
                     SHAPAAAGGRATLAHASAALMQALGERVILTVDGELDQDPDRRRHAWPGAVLAGAIWT
                     ARPGLDLDVGYQASTHASPALHEWLAGITYRFAL"
     gene            912..>1680
                     /gene="pstS"
                     /locus_tag="VF453_20085"
     CDS             912..>1680
                     /gene="pstS"
                     /locus_tag="VF453_20085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX7690017.1"
                     /translation="MLRRFLRLALLPAFASLLAAAAPAQSLHGAGATFPAPVYQAWAE
                     EWQRRTGEAVRYDELGSGAGIAAATQGQVDFGASDAPLADDRLAAADLLQFPTVIGGV
                     VPVVNVAGVAHGRLQLDAAALSAIYRGRIVNWSDPAIAALNPGLALPDARITVVHRAD
                     ASGTTWLWSRWLSESDAAWKAEVGQGTTLAWPTGTGGLGNEGVASLVRRTRGAIGYVE
                     AAYARAHELADVALPARDGGLVRASPESFEAALASARW"
BASE COUNT          198 a          634 c          636 g          212 t
ORIGIN      
        1 gccaggttct cagagcgcga accggtaggt gatgccggcc agccactcgt gcagcgccgg
       61 cgaggcatgc gtgctggcct ggtagccgac gtccagatcc agcccaggcc gcgccgtcca
      121 gatcgcgccg gccagcacgg cacccggcca ggcgtggcgg cgacgatccg gatcctggtc
      181 gagctcgccg tccaccgtca ggatcacgcg ctcgcccagc gcctgcatca gcgccgccga
      241 cgcatgcgcg agcgtcgcgc gcccacccgc ggcggccggc gcatgcgaga agccgaggtt
      301 ggcgtgcacg gtcgtgggcg aggcatccca ggtggcgacc gccagcgcat gctccgcgat
      361 ccgcccgtgc ggcagcccga ggccgtgccg cgccgtcggc gcctcgatgc cggcgcgcac
      421 gccgagcgac cacggatcgg cgccccagaa gcgccacttg gcgtcgaggt tggtgtcgcc
      481 gacgccggcg ctcgtccctt gcgcgggatc gtgctgcgag acccaggagg gctgcacgat
      541 cgcgtcgacc gtcgtcgcga ggcccacgga gacctgcggc tggtaggtga aggtcgtgac
      601 accgccgacg cgcgcgcgct ggaacccgtt ctcgatctcg acgttgccgg cgccctgggt
      661 gccggtgtcc tcggtctgca gcgggtgggc ggcgaatccg ggggcggcga aggccgcaag
      721 gagcgcgggc aggcagcggg tcgggcggcg gaggatcacg tggcgcatgg cggggggaag
      781 gcgcaggcag tcagtctgtc cgggcgcgat gacagcgtcg tgtcgtgctg tcacgcgccc
      841 gccatgcggc ggcgggagac tgctcgattg gccgcgtgca tcgcgcgtct tcagaccccg
      901 acagcctgcc catgctccga cgcttcctcc gcctcgccct gctccccgcc tttgcgtccc
      961 tcctggccgc cgccgcgccc gcgcagtcgc tgcacggcgc cggcgcgacc ttcccggcgc
     1021 ccgtctacca ggcctgggcc gaggagtggc agcgccgcac cggcgaggcc gtgcgctacg
     1081 acgagctcgg ctcgggcgcc gggatcgccg cggcgacgca gggccaggtg gacttcggcg
     1141 cctccgacgc gccgctggcc gacgaccggc tcgccgccgc cgacctgctg cagttcccga
     1201 cggtgatcgg cggcgtcgtg ccggtcgtca acgtcgcggg cgtggcgcac gggcgcctgc
     1261 agctcgacgc cgcggcgctc agcgcgatct accgcggccg catcgtcaac tggagcgatc
     1321 cggcgatcgc ggcgctcaac cccgggctcg cgctgccgga tgcgcggatc acggtcgtcc
     1381 accgcgccga cgcctccggc acgacctggc tgtggagccg gtggctctcg gagtcggacg
     1441 ccgcgtggaa ggccgaggtc ggccagggta cgacgctcgc atggcccacc ggcaccggcg
     1501 gcctgggcaa cgagggcgtc gcctcgctgg tgcggcgcac gcgcggcgcg atcggctatg
     1561 tcgaggcggc ctatgcgcgc gcccacgagc tcgccgacgt cgccctgccc gcgcgcgacg
     1621 gcggcctggt gcgcgcgagc cccgagagct tcgaggccgc gctcgcctcg gcccgctggc
//
LOCUS       DASVLN010000853         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_205612, whole genome shotgun sequence.
ACCESSION   DASVLN010000853 DASVLN010000000
VERSION     DASVLN010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205612"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1134
                     /locus_tag="VF453_20090"
     CDS             346..1134
                     /locus_tag="VF453_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7690018.1"
                     /translation="MHFDLPTGVAGLLVGAIVGVTGVGGGALMTPILVLLFGVAPTTA
                     VGTDLLYASITKSAGTAVHQSHGTVDWQVVRRLACGSLPAAALTLAWMHFGHEGPLKS
                     GVVLFTLGVALLITALGILFKDRIHDIGTRSRLGNSVRFKSWQPGLTVASGALLGVLV
                     TMTSVGAGALGTAMLVYLYPLRLKAGKLVGTDLAHAIPLTLIAGLGHLTLGNVDLGLM
                     LNLLAGSIPGVIAGSLVSARAPVKVVRYAIAIVLLFVAWKMVTR"
     gene            complement(1138..>1732)
                     /locus_tag="VF453_20095"
     CDS             complement(1138..>1732)
                     /locus_tag="VF453_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690019.1"
                     /translation="KGGLAPPPRPRGDAVITIADRASAAGSGAEGYHDLGRGEVAVGF
                     VFLDVCRAAGDAWTAALSHEAIELTADPLNNLLVQGPHPRDRRRQVFHAFELCDAVQA
                     EGYEIKGVAVSNFLLPGWFATPAIAGGRNDFLGRPVRGEVLAPFHMAPGGSLTFYDPA
                     LKRAKWTTATAPDDDAAARRVAAKRAAGLTRIARRER"
BASE COUNT          206 a          627 c          638 g          261 t
ORIGIN      
        1 gacggatcgg aacgaggcca gcgacggggc cgccacgcgt cgcgcggcgc cgcgggcgcg
       61 ggtgccggac tggccagcgg cgggtgcgga catcggcctt caggggatga atcgagcctg
      121 catcctaccc ggcccgccgc gtgccccggc acggatgcag atgcttcctc ggttttcgca
      181 taacgaggtc attgcccggc acccagaatg gcgcctcgcg gcctcccacc gccaggcgcg
      241 ccgtcaagcg ccggtggaga ccgccgcccc ccgtcgccgg ccgcgcccat cgcggccaac
      301 ccgtccgtcg ttttcctcgt ccgccccggc ggatcgccct gtcccgtgca tttcgatctc
      361 ccgaccggcg tggccggcct gctcgtgggc gccatcgtgg gcgtcacggg cgtcggcggt
      421 ggcgcgctga tgacgccgat cctcgtcctg ctcttcggcg tcgccccgac gaccgccgtt
      481 ggaaccgacc tgctgtacgc ctcgatcacc aagtccgccg gcaccgccgt gcaccagtcg
      541 cacggcaccg tcgactggca ggtcgtccgg cgcctggcct gcggaagcct gcccgcggcg
      601 gcgctcacgc tcgcctggat gcatttcggc cacgagggcc cgctgaagtc gggcgtcgtc
      661 ctgttcacgc tcggcgtggc gttgctgatc accgcgctcg ggatcctgtt caaggaccgc
      721 atccatgaca tcggcacgcg ttcgcgcctg ggcaacagcg tgcgattcaa gtcctggcaa
      781 ccgggcttga ccgtggcctc gggggcgttg ctgggcgtgc tggtgacgat gacctcggtc
      841 ggcgccggcg cgctgggcac ggcgatgctg gtctacctct acccgctgcg gctgaaggcg
      901 ggcaagctgg tcggcacgga cctcgcgcat gcgatcccgc tcaccctgat cgccggcctc
      961 ggccacctga cgctgggcaa cgtggacctg ggcctgatgc tcaacctgct ggccggctcg
     1021 attccgggcg tgatcgcggg gtccctcgtg agcgcccgcg cgccggtgaa ggtggtgcgc
     1081 tacgcgatcg cgatcgtgct gctgttcgtc gcgtggaaga tggtcacgcg ctgaaggtca
     1141 gcgctcgcgg cgggcgatgc gcgtgagtcc ggcggcgcgc ttggccgcga cgcggcgcgc
     1201 ggcggcgtcg tcgtcgggcg cggtggcggt cgtccacttc gcgcgcttca gggccgggtc
     1261 gtagaaggtc aggctgcccc cgggggccat gtggaagggc gcgagcacct cgccgcgcac
     1321 cgggcgtccg aggaagtcgt tgcgcccgcc ggcgatggcg ggcgtcgcga accaaccggg
     1381 cagcaggaag ttggagacgg ccacgccctt gatctcgtag ccctccgcct gcacggcgtc
     1441 gcacaactcg aacgcgtgga agacctggcg tcgccggtcg cgcggatgcg ggccctgcac
     1501 gagcaggttg ttcagcggat cggcggtgag ctcgatcgcc tcgtgcgaca gcgccgccgt
     1561 ccacgcgtcg cccgcggcgc ggcagacgtc gaggaagacg aagcccaccg cgacctcgcc
     1621 gcgcccgagg tcgtggtagc cctcggcgcc cgagccggcg gcgctcgcgc ggtcggcgat
     1681 ggtgatgacg gcgtcgccgc gtgggcgcgg gggcggcgcc aggccgccct tc
//
LOCUS       DASVLN010000854         4502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_43006, whole genome shotgun sequence.
ACCESSION   DASVLN010000854 DASVLN010000000
VERSION     DASVLN010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43006"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1159
                     /gene="mgrA"
                     /locus_tag="VF453_20100"
     CDS             110..1159
                     /gene="mgrA"
                     /locus_tag="VF453_20100"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEX7690020.1"
                     /translation="MTYAADHDRYDGRMPYRPCGRSGLRLPAISLGLWHNFGDTTPMV
                     NQRQMLRTAFDHGITHFDLANNYGPPYGSAETNFGEHLRRDFRPYRDELIISSKAGWD
                     MWPGPYGQGGGSRKYVLASLDQSLKRLGLDYVDIFYSHRFDPDTPLEETMGALASAVR
                     QGKALYVGISSYSAARTREAAALLAGMGVTALIHQPSYSMLNRWVEGELLDALDRAGM
                     GCIAFSPLAQGLLTDKYLHGIPEDSRINRPGGSSLQASHLSEANLEHVRALNRIAQDR
                     GQTLAQMAVAWVLRDRRVTSALVGASHPGQIVDLAKAVQRLDFSADELAAIDRHAVEG
                     GINLWQKPSTDQRPD"
     gene            1225..1842
                     /locus_tag="VF453_20105"
     CDS             1225..1842
                     /locus_tag="VF453_20105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX7690021.1"
                     /translation="MSNPLSHEFEHDLQGYKVAILVDDFFEQAELEEPLKALKNAGAQ
                     TFIISPKGPTLTGMKHHDKGDSFPVDVPLDHADPSDYDALVLPGGVVNADALRIDPAA
                     QQFARAMDRANKPLAVICHGAWLLISAGLVKDRTLTSWPTLKDDLVNAGAKWVDKEVV
                     SDRHWTSSRKPADLPAFNKTFMETLTSIAPMWATQPRAGQPVSNH"
     gene            1897..2331
                     /locus_tag="VF453_20110"
     CDS             1897..2331
                     /locus_tag="VF453_20110"
                     /inference="COORDINATES: protein motif:HMM:NF025444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HEX7690022.1"
                     /translation="MGTWSHDAFGNDEACDCALALARGRDLSAVEGALDDVAGFPPHE
                     YLETPEATQALVAAEAVARLRGHPGERDACSKPVDDWVARTQLAASDALAARAVEAIE
                     RILGAASELADLWGDSPDDDARWRASMHALKVRLLAPARPVA"
     gene            2360..3448
                     /locus_tag="VF453_20115"
     CDS             2360..3448
                     /locus_tag="VF453_20115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEX7690023.1"
                     /translation="MTAPHADDLVIARPQGLYCPPGDFYIDPWQPVDHALITHAHGDH
                     LRAGNARYLAAEAGRHVIEARVGPVALQTLPYGQTLQHRGVTISFHPAGHVLGSAQIR
                     LEHAGRRWVVSGDYKTGPDATCAPFEPVRCDVFVTESTFGLPVFRWVPQQAVFDEINA
                     WWQANADAGRASIVHAYAFGKAQRVLSGLDASIGPVVVHGAVATMDEAYRRSGVALPA
                     TMRVDDATPEQLRRSLVVAPPSAQGSAWSRRFSRGGDASDSFASGWMQLRGARRRRNV
                     DRGFVLSDHADWPGLLEAIAATGAPRVIVTHGYVEPLVRWLASQGLDAGAFRTEYGDD
                     EETRDAAASGPEPEPVPVSESGPEGDAS"
     gene            3445..>4502
                     /locus_tag="VF453_20120"
     CDS             3445..>4502
                     /locus_tag="VF453_20120"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351230.1"
                     /note="catalyzes the ATP-dependent formation of a
                     phosphodiester at the site of a single strand break in
                     duplex DNA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX7690024.1"
                     /translation="MKDFAALFAELDATTSTRRKTDALARYFRRARPADAAWALYFLA
                     GGKPRQTVPTALLRAAARERAGIPEWLFDESYSVVGDLAETIAHVLPPAGPAVDLPLH
                     TWIEERLIALRGREPAQVVAALQGYWDELDTPGRFLLTKLIGGGFRVGVSRLLVVRGL
                     AEATGLDPKLLAQRLVGYTDKRHEPDAARYEALVDPEVGDRGDEGRPYPFFLAHPLQA
                     PVETLGAREDWLVEWKWDGIRAQFIRRGARSWLWSRGEDLLNGRFPEIDDAHALLPDG
                     TVLDGEVVVWRDGRVQPFARLQQRIGRKNVTRKILADHPAAFLAYDLLEEAGADLRAA
                     PQRERRTRLEALLARVAAA"
BASE COUNT          643 a         1724 c         1565 g          570 t
ORIGIN      
        1 cgggaaagtc cgcgcccgcg ggcggtcgcg cgcgccgcgc gccagaatct ccgcgccgcc
       61 gccgccgccg ctcgcgcgcg ccgtccacgt ccacccgagg agcccgccca tgacctacgc
      121 cgccgaccac gaccgctacg acggccgcat gccctaccgc ccctgcggcc gcagcggcct
      181 gcgcctgccg gcgatctcgc tgggcctgtg gcacaacttc ggcgacacga cgccgatggt
      241 caaccagcgc cagatgctgc gcacggcgtt cgaccacggc atcacgcact tcgacctcgc
      301 caacaactac gggccgccct acggcagcgc cgagaccaac ttcggcgagc acctgcggcg
      361 cgacttccgg ccctaccgcg acgagctgat catttccagc aaggccggct gggacatgtg
      421 gccgggcccg tacggccagg gcggcggctc gcgcaagtac gtgctggcga gcctcgacca
      481 gagcctgaag cgcctggggc tggactatgt cgatatcttc tactcgcacc gcttcgaccc
      541 ggacacgccg ctcgaggaga cgatgggcgc gctggccagc gccgtgcgcc agggcaaggc
      601 gctgtacgtg ggcatcagca gctactcggc ggccaggacg cgcgaggccg cggcgctgct
      661 ggccggcatg ggcgtgacgg cactgatcca ccagccgtcg tacagcatgc tcaaccgctg
      721 ggtcgagggc gagctgctcg atgcgctcga ccgcgccggc atgggctgca tcgccttcag
      781 cccgctcgcg cagggcctgc tgacggacaa gtacctgcac ggcatccccg aggactcgcg
      841 catcaaccgg cccggcggca gctcgctgca ggcgtcgcac ctgagcgagg ccaacctcga
      901 gcacgtgcgg gcgctgaacc gcatcgcgca ggatcgcggg cagacgctcg cgcagatggc
      961 cgtcgcctgg gtgctgcgcg accggcgcgt gacctcggcg ctcgtcggcg cgagccatcc
     1021 ggggcagatc gtcgacctcg cgaaggccgt ccagcgcctc gacttcagcg ccgacgagct
     1081 ggccgcgatc gacaggcacg cggtcgaggg cggcatcaac ctctggcaga agccttcgac
     1141 cgaccagcgg cccgactgac gggcacggct cgggcacgca ccctgcccga tcctgcgtca
     1201 tccacgaacg caggagcaga tgccatgagc aatcccctca gccacgaatt cgagcacgac
     1261 ctccagggct acaaggtcgc gatcctcgtc gacgacttct tcgagcaggc cgagctcgag
     1321 gagccgctga aggcgctgaa gaacgccggc gcgcagacct tcatcatctc gcccaagggc
     1381 ccgacgctga ccggcatgaa gcaccacgac aagggcgact cgttcccggt cgacgtgccg
     1441 ctggaccatg ccgacccctc cgactacgat gcgctcgtgc tgccgggcgg cgtcgtcaac
     1501 gccgacgcgc tgcgcatcga cccggcggcc cagcagttcg cccgcgccat ggatcgcgcc
     1561 aacaagccgc tggcggtgat ctgccacggc gcgtggctgc tgatctccgc ggggctcgtc
     1621 aaggaccgca cgctgacgag ctggcccacg ctgaaggacg acctcgtcaa tgccggcgcc
     1681 aagtgggtgg acaaggaggt ggtgagcgac cgccactgga cgtccagccg caagccggcc
     1741 gacctgcccg cattcaacaa gaccttcatg gagacgctga cctccatcgc gccgatgtgg
     1801 gcgacccagc cccgcgccgg ccagcccgtc agcaaccact gaagcgcgac gcgggcctaa
     1861 gatgcgtccc gacgccacga cgcgggacgc atcgccatgg gaacctggag ccacgacgcc
     1921 ttcggcaacg acgaggcctg cgactgtgcc ctcgcgctgg cccgcggccg cgacctgtcg
     1981 gccgtcgagg gcgcgctcga cgacgtggcc ggcttcccgc cgcacgagta cctcgagacg
     2041 cccgaggcca cgcaagccct ggtcgcggcc gaggccgtcg cccgcctgcg cggccatccg
     2101 ggcgagcgcg acgcctgcag caagccggtc gacgactggg tcgcgcgcac gcagctcgcc
     2161 gcatccgatg ccctggccgc gcgcgcggtc gaggcgatcg agcgcatcct cggcgcggcg
     2221 tcggagcttg ccgacctctg gggcgactcg cccgacgacg acgcgcgctg gcgcgcctcg
     2281 atgcatgcgc tgaaggtgcg gctgctcgca ccggcgcgac ccgtcgcgtg atggcacgga
     2341 cgttgccgaa cgcccggtga tgaccgcgcc ccacgccgac gacctcgtga tcgcccgccc
     2401 ccagggcctg tactgcccgc cgggcgactt ctacatcgac ccgtggcagc cggtcgacca
     2461 cgcgctgatc acccatgccc acggcgacca cctgcgcgcc ggaaacgcgc gctatctcgc
     2521 cgcggaggcc gggcgtcacg tgatcgaggc gcgcgtcggg ccggtcgcgc tgcagacgct
     2581 gccgtacggc cagacgctgc aacaccgcgg cgtcacgatc tcgttccacc ccgccggcca
     2641 cgtgctgggc tcggcgcaga tccggctcga acacgcgggc cggcgctggg tcgtctcggg
     2701 cgactacaag accggccccg acgcgacgtg tgcgccattc gagcccgtgc gctgcgatgt
     2761 cttcgtcacc gagtcgacgt tcggcctgcc ggtgttccgc tgggtgccgc agcaggccgt
     2821 cttcgacgag atcaacgcgt ggtggcaggc caacgcggac gccggtcgcg cgtccatcgt
     2881 ccacgcgtac gcgttcggca aggcgcagcg ggtgctgtcg ggactcgacg cctcgatcgg
     2941 gcccgtcgtc gtgcacggcg ccgtcgcgac gatggacgag gcctaccggc gctccggcgt
     3001 cgcgctgccc gccacgatgc gcgtcgacga cgcgacgccc gagcagctgc ggcgcagcct
     3061 cgtcgtcgcg ccgccgtcgg cgcagggcag cgcctggtcg cgccgcttca gccgcggcgg
     3121 cgatgccagc gactcgttcg cgtccggctg gatgcagctg cgcggcgcgc gccggcggcg
     3181 caacgtcgac cgcggcttcg tgctgtccga ccatgccgac tggcccggcc tgctcgaggc
     3241 gatcgccgcc accggggcgc cgcgcgtgat cgtcacgcac ggctacgtcg agccgctggt
     3301 gcgctggctc gcctcgcagg gcctcgacgc gggcgcgttc cgcaccgagt acggcgacga
     3361 cgaggagacg cgcgacgccg ccgcgtccgg gccggaaccc gaacccgtac ccgtgtcgga
     3421 atccgggccg gaaggcgacg cgtcgtgaag gacttcgccg ccctcttcgc cgagctcgac
     3481 gccacgacct cgacgcgtcg caagaccgac gcgctggccc gctacttccg ccgcgcgcgt
     3541 ccggccgacg ccgcgtgggc gctgtacttc ctggccggcg gcaagccgcg ccagacggtg
     3601 ccgaccgccc tgctgcgcgc cgccgcgcgc gagcgcgccg gcatcccgga atggctcttc
     3661 gacgaaagct actccgtcgt cggcgacctg gcggagacca tcgcccacgt cctgccgccc
     3721 gcaggccccg ccgtcgacct gccgctgcac acgtggatcg aggagcgcct gatcgcgctg
     3781 cgcggccgcg agccggcgca ggtcgtggcg gctctgcagg gctattggga cgagctcgac
     3841 accccgggcc gcttcctcct gacgaagctg atcggcggcg gattccgggt gggcgtctcg
     3901 cggctgctgg tggtgcgcgg gctcgccgag gcgaccggcc tcgatcccaa gctgctcgcg
     3961 cagcgcctcg tcggctacac cgacaagcgg cacgagcccg acgccgcgcg ctacgaggcg
     4021 ctggtcgacc ccgaggtcgg cgaccgcggc gacgagggcc ggccctaccc gttcttcctc
     4081 gcccatccgc tgcaggcgcc ggtcgagacg ctgggggcgc gcgaggactg gctcgtcgag
     4141 tggaagtggg acggcatccg cgcgcagttc atccgccgcg gcgcgcgatc gtggctgtgg
     4201 tcccgcggcg aggacctgct gaacggccgc ttcccggaga tcgacgatgc ccatgccctg
     4261 ctgccggacg gcaccgtgct ggacggcgag gtcgtggtgt ggcgcgacgg ccgcgtgcag
     4321 ccgttcgcgc ggctgcaaca gcgcatcggg cgcaagaacg tcacgaggaa gatcctcgcc
     4381 gaccaccccg ccgccttcct cgcctacgac ctgctcgaag aggcgggcgc cgacctgcgc
     4441 gccgcgccgc agcgcgagcg gcgcacgcgc ctggaggcgc tgctcgcgcg cgtcgccgcg
     4501 gc
//
LOCUS       DASVLN010000855         5225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_475573, whole genome shotgun sequence.
ACCESSION   DASVLN010000855 DASVLN010000000
VERSION     DASVLN010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475573"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1139)
                     /locus_tag="VF453_20125"
     CDS             complement(156..1139)
                     /locus_tag="VF453_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7690025.1"
                     /translation="MSAALLEVRELRVDFPQRRGGALRALDGVSFDIAPGEILGVVGE
                     SGAGKSLTGAAIIGLLDAPGRVSGGEILLDGQRIDDLPPERMRRLRGRQIGAIFQDPL
                     SSLNPLYTVGRQLVETLRTHLPLTRAQARERAIDLLRQTGIPAPEARLDQYPHEFSGG
                     MRQRVVIALALAGEPRLIIADEPTTALDVAVQAQIMALLRKVSQAHGAAVMLITHDMG
                     VIAETCDRVAVMYAGRVVEIGPVREVIHRPAHPYTAGLMASVPRLDGAVGRLAQIEGA
                     LPRLDAIPPGCAFHPRCPKASLRCEVERPEPALVRRTRAACWWPLDDRAIA"
     gene            complement(1168..2079)
                     /locus_tag="VF453_20130"
     CDS             complement(1168..2079)
                     /locus_tag="VF453_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7690026.1"
                     /translation="MPSRLRRLLGSDLWYDFTHSPVAMVAAAVALLCAACAAFAPWIA
                     PHDPFDLSTLDLSDSHLPPAWLPDGRSAFPLGTDDQGRDILSALMYGARISLLVGLGS
                     VLLSMAFGVVAGLVAGFIGGVVDAVLMRLCDVTLSFPAILVALLIDGVGRVVFPHARD
                     VLAFLVLIFSLTATGWVSYARTVRGTTLVERHKEYVQAARLIGVPRWRILRRHVLPNV
                     LGPVLVLATIQVGQAIIAEATLSFLGVGVPPTNPSLGTLIRVGNDFLYAGEWWITLFP
                     GVALVVIAMSLNLLGDWLRDALNPRLQ"
     gene            complement(2130..3125)
                     /locus_tag="VF453_20135"
     CDS             complement(2130..3125)
                     /locus_tag="VF453_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7690027.1"
                     /translation="MLTYVLRRFAQAVLVLLVVGMGSFALFQYVGDPVNNMVGQDATP
                     EQRQRLRVELGLDQPAPVQFGRFLARAVHGDFGLSLRQGRQVSALIAESLPATLELSF
                     AAAVIATVFGIPLGVFAALRRGRWDAQLAMVVSLLGVSLPTFLIGIVLIEVFSVLLGW
                     LPSFGRGSTVQLGGWSTGLATADGWRHLVLPAITLAVFQLTLVMRLVRAEMLEALRSD
                     YVRFARARGLPDRAVHFGHALRNTLIPVLTIAGLQLGGLIAFSIITETVFAWPGVGSL
                     FIQAVQFPDVPVMAAYLCLVALMFVVINLVVDLLYVLVDPRLRVRGSPPAAGGGR"
     gene            complement(3275..4297)
                     /locus_tag="VF453_20140"
     CDS             complement(3275..4297)
                     /locus_tag="VF453_20140"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7690028.1"
                     /translation="MKKSLLALAVLTAIAGAAQAQSSVTLYGKVDLGGVLDSGSKNGK
                     SVRLDSGVSGGSRIGFKGVEDLGGGTKVSFQLETGYCADSAAGAPNFCTGSNQFMGRQ
                     AHGDLTGAFGALSAGRQYSLGFNNLSTIDPFGTGFAGQVNNIVNPSGIRLNNSVTYTT
                     PSIAGLTASGEVAFGEQTGNWEANRETGAAVTYANGPAYAGFTFYDQGNANGDGAARK
                     NFLLGGTYDFGVVKVHALVQKGSGQPTGGAKVDVLDYMGGVTVPLAGGSLLASVVHEN
                     DKTVANKDATQYGVGYLYPLSKRTSVYTAFGKINNQHGATFTVGNATAAGTGNKAFDL
                     GVVHNF"
     gene            4619..>5225
                     /gene="coq7"
                     /locus_tag="VF453_20145"
     CDS             4619..>5225
                     /gene="coq7"
                     /locus_tag="VF453_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEX7690029.1"
                     /translation="MELSLLDRWLCGAETALRTLSGGIRAERPSPAPEPGADPAASAE
                     LSPEERRLSGALMRVNHVGEVCAQALYQAQALATSDPGLRSKMAQAAREEIDHLAWTS
                     ARLAELGDRPSLLNPLWYGGAFLIGLAAGRLGDRVSLGFVVETERQVEEHLAGHLERL
                     PSGDAASRAIVARMKDDEAAHAQAAADAGGAALPAPVRAAMR"
BASE COUNT          819 a         1844 c         1892 g          670 t
ORIGIN      
        1 ctgcacccgg cgcggctcgg ctcggcgcag cgcgcgctcg atccacggcg tcgcgatctc
       61 gaagctgcgc gcgagtccgc gcgcctcgac cagggcgccg cgcggcccgc tcgcctgcac
      121 ggtcgccgca gggacgccct cgccgatgtc gcgcgtcatg cgatcgccct gtcgtcgagc
      181 ggccaccagc acgccgcgcg cgtgcgtcgc acgagcgccg gttccgggcg ctcgacctcg
      241 cagcgcaggc tggccttcgg gcagcgcggg tggaacgcgc aacccggcgg gatcgcgtcc
      301 aggcgcggca gcgcgccctc gatctgcgcg aggcggccga ccgcgccatc cagccgcggc
      361 accgaggcca tgaggcctgc cgtgtacgga tgcgccggcc ggtggatcac ctcccgcacg
      421 gggccgatct cgacgacgcg ccccgcgtac atcacggcca cgcgatcgca ggtctctgcg
      481 atgacgccca tgtcgtgcgt gatcagcatg acggccgcgc cgtgcgcctg ggagaccttg
      541 cgcagcagcg ccatgatctg cgcctgcacg gccacgtcga gcgcggtcgt cggctcgtcg
      601 gcgatgatca gccgcggctc gccggccagc gccagcgcga tgacgacgcg ctggcgcatg
      661 ccgccggaga actcgtgcgg atactggtcg aggcgcgcct cgggcgcggg gatgccggtc
      721 tggcgcagca ggtcgatcgc gcgctcgcgc gcctgagccc tcgtcagcgg caggtgcgtg
      781 cgcagcgtct ccaccagctg gcgaccgacc gtgtacagcg ggttcagcga gctcagcgga
      841 tcctggaaga tcgcgccgat ctgcctgccg cgcaggcgcc gcatgcgctc gggcggcagg
      901 tcgtcgatgc gctggccatc gagcaggatc tcgccgccgc tcacgcgacc cggggcgtcg
      961 agcaggccga tgatggccgc gccggtcagc gacttgcccg cgccggactc gcccacgacg
     1021 ccgaggatct cgcccggcgc gatgtcgaag ctcacgccgt cgagcgcgcg cagcgcgccg
     1081 ccgcgccgct gcgggaagtc gacgcgcagc tcgcgcacct cgagcagggc ggcgctcacg
     1141 ggcggactcc gtcgacggcg cgcgggctca ctgcagcctc gggttgaggg cgtcgcgcag
     1201 ccagtcgccc agcaggttca agctcatcgc gatcaccacc agcgcgacgc cgggaaagag
     1261 cgtgatccac cactcgcccg cgtagaggaa gtcgttgccg acgcggatca gcgtgcccag
     1321 agacgggttg gtcggcggca cgcccacgcc gaggaacgac agcgtcgcct ccgcgatgat
     1381 cgcctggccg acctggatgg tcgccagcac cagcacgggc ccgagcacgt tgggcaggac
     1441 gtggcggcgc aggatgcgcc agcgcggcac gccgatcagc cgcgcggcct gcacgtattc
     1501 cttgtgacgt tcgacgaggg tcgtgccgcg caccgtgcgt gcgtaggaca cccagccggt
     1561 ggccgtgagc gagaagatca gcaccaagaa ggcgagcacg tcgcgcgcat gcgggaagac
     1621 gacgcgaccg acgccgtcga tcagcaacgc gacgaggatg gccgggaacg acagcgtgac
     1681 gtcgcacagc cgcatcagaa ccgcgtcgac gaccccgccg atgaagcccg ccaccagccc
     1741 cgcgacgacg ccgaaggcca tcgacagcag caccgaaccc agtccgacca gcagcgagat
     1801 gcgcgcgccg tacatcaggg cggagaggat gtcgcggccc tggtcgtcgg tgccgagcgg
     1861 gaaggcgctg cggccatccg gcagccaggc gggcggcagg tgcgagtcgg acaggtcgag
     1921 cgtcgacagg tcgaacgggt cgtgcggcgc gatccacggc gcgaacgccg cgcacgccgc
     1981 gcacagcagc gccaccgccg cggccaccat cgcgaccggc gaatgcgtga agtcgtacca
     2041 gagatcgctg ccgaggagtc gtcgcaggcg cgagggcatc gtcgccgtga cggccacctc
     2101 ctgcacctgc gccggcggga tgcggtcgct caacgcccgc ccccggccgc cggcgggctg
     2161 ccgcgcacgc gcagccgcgg gtcgacgagc acgtacagca ggtcgaccac caggttgatc
     2221 acgacgaaca tcagcgccac caggcagagg taggccgcca tgacgggcac gtcggggaac
     2281 tgcacggcct ggatgaagag gctgcccacg ccgggccatg cgaagacggt ctcggtgatg
     2341 atggagaacg cgatcagtcc gcccagttgc aggccggcga tcgtcagcac cgggatcagc
     2401 gtgttgcgca gcgcatggcc gaagtgcacc gcgcgatcgg gcagcccgcg ggcgcgcgcg
     2461 aagcggacgt agtcggagcg cagcgcctcc agcatctcgg cgcgcacgag ccgcatgacg
     2521 agcgtgagct ggaacaccgc cagcgtgatc gccggcagca cgagatggcg ccagccgtcc
     2581 gcggtcgcga gcccggtgct ccatccgccg agctgcaccg tgctgccgcg gccgaagctg
     2641 ggaagccagc cgagcagcac cgagaacacc tcgatcagca cgatgccgat caggaaggtc
     2701 ggcagcgaca cgcccagcag cgacaccacc atcgccagct gcgcgtccca acgtccgcgg
     2761 cgcaacgcgg cgaatacccc aagcggtatg ccgaagacgg tcgcgatgac ggccgcggcg
     2821 aaggacagct ccagcgtcgc cggcaggctc tcggcgatca gtgcggaaac ctggcgcccc
     2881 tggcgcaggc tcaagccgaa gtcgccgtgc accgcgcgcg cgaggaagcg gccgaactgc
     2941 acgggcgccg gctggtcgag gcccagctcc acgcgcagcc gctggcgctg ctcgggcgtg
     3001 gcgtcctgcc ccaccatgtt gttgacggga tcgccgacgt actggaagag cgcgaacgag
     3061 cccatgccga cgaccagcag cacgagcacg gcctgggcga atcgacggag cacgtaggtc
     3121 agcatcggac tcgaagaata cccgcagccg gcggcgccgc cactggcgtc gcccctcggt
     3181 cgcgcgaatt cctgcccggc ccgggaatga aaaaggcccg tccggagacg ggccttcgag
     3241 cgaaacgcga accggcgcga aggccggcgg cggatcagaa gttgtggacg acgcccaggt
     3301 cgaacgcctt gttgcccgtg ccggcagcgg tcgcgttgcc gaccgtgaag gtggcgccgt
     3361 gctggttgtt gatcttgccg aacgcggtgt agaccgaggt gcgcttcgac agcgggtaca
     3421 ggtagcccac gccgtactgg gtcgcgtcct tgttggccac ggtcttgtcg ttctcgtgca
     3481 cgaccgaggc cagcagcgaa ccgccggcca ggggcacggt cacgccgccc atgtagtcga
     3541 ggacgtcgac cttggcgccg cccgtcggct ggccggagcc cttctgcacc agggcgtgga
     3601 ccttgacgac gccgaagtcg tacgtgccgc ccagcaggaa gttcttgcgc gcggcgccgt
     3661 cgccattggc gttgccctgg tcgtagaacg tgaagccagc ataggccggg ccgttcgcgt
     3721 aggtcacggc ggcgcccgtt tcgcggttgg cttcccagtt gccggtctgc tcgccgaagg
     3781 cgacttcacc cgacgccgtc aggccggcga tcgaaggcgt ggtgtacgtg accgagttgt
     3841 tcaggcggat gcccgacggg ttcacgatgt tgttgacctg gccggcgaag cccgtgccga
     3901 acgggtcgat cgtgctcagg ttgttgaacc cgaggctgta ctggcggccg gcgctcaggg
     3961 caccgaaggc gccggtcagg tcgccgtgag cctggcggcc catgaactgg ttggagcccg
     4021 tgcagaagtt cggggcgccg gcggcgctgt cggcgcagta gccggtttcc agctggaacg
     4081 acaccttggt gccaccgccg aggtcttcaa cacccttgaa gccgatgcgc gaaccgccgg
     4141 acacgccgga gtccagacgc acggacttgc cgttcttgga gccgctgtcg agcacgccac
     4201 cgaggtcgac cttgccgtac agcgtgacgg aggactgggc ttgggcggcg ccggcgatgg
     4261 cggtgagcac tgcgagagcg agcagcgact ttttcattgc gaatatctcc tagggttaac
     4321 aagagattcc ggtcgcggag gcggctgggc gatactttgt tttcaccgct tggtaccgca
     4381 acctcatcgg aagctggacg tattgcacca gacgcgccga gcgcttgcct agctccacaa
     4441 cggaaaatcg gccgtgcttg ttgtctgcct gcaacgacgc cacggcagag tccgcagccc
     4501 ggcggatgcc tgcgcacggg cgtgcgacag ggctgtgacg gctcagtgac gacgcggccg
     4561 cgccgggcct cgcctgcgcg ccgcgaggga ccgcgcgcga tggggtatcg tgccgctcat
     4621 ggaactgagc ctcctcgatc gctggctgtg cggggcggag acggcgttgc gcacgctgtc
     4681 cggcggcatc cgcgcggagc gcccctcgcc cgcgccggaa cccggcgccg accccgcggc
     4741 ctcggccgag ctgagccccg aggagcgccg cctctcgggg gcgctgatgc gcgtcaacca
     4801 cgtcggcgag gtgtgcgcgc aggcgctgta ccaggcgcag gcgctcgcga cctccgaccc
     4861 aggcctgcgc tcgaagatgg cgcaggcggc ccgcgaggag atcgaccacc tcgcctggac
     4921 ctctgcccgc ctggccgagc tcggcgaccg tccgagcctg ctgaacccgc tgtggtacgg
     4981 cggcgcgttc ctgatcggcc ttgcggccgg caggctgggc gatcgcgtca gcctcggctt
     5041 cgtggtcgag accgaacgcc aggtcgagga acacctcgcc ggccacctgg agcgcctccc
     5101 gagtggcgac gcggcgtcgc gcgcgatcgt cgcgcggatg aaggacgacg aggcggccca
     5161 tgcgcaggcc gccgccgacg cgggcggggc ggcgctgccg gccccggtgc gcgccgccat
     5221 gcgcg
//
LOCUS       DASVLN010000856         2379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_99188, whole genome shotgun sequence.
ACCESSION   DASVLN010000856 DASVLN010000000
VERSION     DASVLN010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99188"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1697)
                     /gene="trpE"
                     /locus_tag="VF453_20150"
     CDS             complement(222..1697)
                     /gene="trpE"
                     /locus_tag="VF453_20150"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX7690030.1"
                     /translation="MITELEFKSLAGQGFNRIPMIFEAFADLETPLSLYLKLAGGQSQ
                     SFLLESVVGGERFGRYSFIGLPARTRVQATGWTTEVVTDGRVVETHEGNPLDFIEAYQ
                     GRFKVALRPGLPRFCGGLAGYFGYDAVRYIEPRLARTTKSGGLDTPDILLLQTEELAV
                     IDNLSGRLYLIVYADPSQPDAYFAAKRRLNELADKLRYSVTAPAVRRGPSYAVQREFA
                     KEAYLDAVLKAKEYIAAGDMMQVQVGQRLSKRYTESPLSLYRALRSLNPSPYMYFYDM
                     GTFQIVGASPEILVRHEHVPEGEKVTIRPIAGTRPRGATPELDAAMEKELLGDPKERA
                     EHLMLIDLARNDVGRIAQTGSVKVTEAFVTERYSHVMHIVSNVEALLKPGMTNMDVLR
                     ATFPAGTLTGAPKIRAMEIIDELEPVKRGIYGGACGYLSYAGDMDVAIAIRTGIVQNN
                     TLYVQAAAGVVADSVPEMEWRETEVKARALLRAAELVEEGF"
BASE COUNT          321 a          836 c          871 g          351 t
ORIGIN      
        1 gccgccgagg ccgcccggcg ccaggcgccc gcgccgacgc gcgcgcgaac gccggagccc
       61 ggcccggcat cggggcgaga cgcagattgc tgccacatgg ggacacctcc acgcgaatgc
      121 ggcgcccgcc gggacggcaa ggcgcaccag ccccgactat gcgccgatgg cggcccttgg
      181 caagcggacg aacgcgcgcc cggcgtgacg gagctcgcgc ttcagaagcc ttcctcgacc
      241 agctcggccg cccgcagcag cgcgcgcgcc ttgacctcgg tctcgcgcca ttccatctcg
      301 ggcaccgagt cggcgaccac gcccgcggcc gcctgcacgt acagcgtgtt gttctgcacg
      361 atgccggtgc ggatggcgat cgccacgtcc atgtcgccgg catagctgag gtagccgcag
      421 gcgccgccgt agatgccgcg cttgacgggc tcgagctcgt cgatgatctc catcgcgcgg
      481 atcttgggcg cgccggtgag cgtgcccgcg gggaacgtgg cgcgcagcac gtccatgttg
      541 gtcatgcccg gcttcagcag cgcctcgacg ttgctgacga tgtgcatgac gtgcgagtag
      601 cgctccgtga cgaaggcctc cgtcaccttg acgctgccgg tctgcgcgat gcggccgacg
      661 tcgttgcgcg cgaggtcgat cagcatgagg tgctcggcgc gctccttcgg gtcgccgagc
      721 agttcctttt ccatcgccgc gtccagctcc ggcgtggcgc cgcgcgggcg cgtgccggcg
      781 atggggcgga tcgtgacctt ctcgccctcg ggcacgtgct cgtggcgcac caggatctcg
      841 ggcgaggcgc cgacgatctg gaacgtcccc atgtcgtaga agtacatgta cggcgacggg
      901 ttgagcgagc gcagcgcgcg gtacaggctc agcggcgact cggtgtagcg cttgctcagg
      961 cgctggccga cctgcacctg catcatgtcg cccgcggcga tgtactcctt ggccttcagc
     1021 accgcgtcga gataggcctc cttggcgaac tcgcgctgca cggcgtacga cggcccgcgg
     1081 cgcaccgccg gcgcggtcac cgagtagcgc agcttgtcgg cgagctcgtt gaggcggcgc
     1141 ttcgcggcga agtaggcgtc gggctgcgac gggtcggcgt agacgatgag gtagagccgg
     1201 cccgacaggt tgtcgatcac cgcgagctcc tcggtctgca gcagcaggat gtcgggcgtg
     1261 tcgaggccgc cggacttggt cgtcctggcg agccggggct cgatgtagcg cacggcgtcg
     1321 tagccgaagt agcctgcgag cccgccgcag aagcgcggca ggcccgggcg cagggcgacc
     1381 ttgaagcggc cctggtaggc ctcgatgaag tcgagcgggt tgccctcgtg cgtctcgacg
     1441 acgcggccgt cggtcacgac ctcggtcgtc cacccggtgg cctgcacgcg ggtgcgcgcg
     1501 ggcaggccga tgaaggagta gcggccgaag cgctcgccgc cgacgaccga ttccagcagg
     1561 aagctctgcg actgcccgcc ggcgagcttg aggtacagcg acagcggcgt ctcgaggtcg
     1621 gcgaaggcct cgaagatcat cgggatgcgg ttgaagcctt ggccggccag gctcttgaat
     1681 tcgagttcgg tgatcacgtt ggcggactcc gggatgccgg gatggcgcgg cgcatcctcc
     1741 gacgaggggc cggcgcgtcc cttggcgacg ttcaggacga cggcgggcgc gcaagccgcg
     1801 ccgtctcggg aagcggcggc gggccggtcg tgcccgcgcc gtcgcgccgt gcgcacccgg
     1861 ccggtgcctc gtgggcggtc gcgggccgga agcgcctcag gcgcgtgcgt tcgtcaggaa
     1921 cggcgtcgcc agggccaggc tccctggtcg tgcgaccgca ccgcgcagcg cggggcgccg
     1981 gtgctggagg agcgcgtggc ttcggacatg cggaccagtc taaccgaagc cgccggccgc
     2041 acgcgcgccg tcgaccgcgc agtgacgtat tgcgcattcg ccaggatttc gagcccgagg
     2101 tcgagcacgg cccggcactt gcttgccgat gcttctcgct aaactgccat ggccgcgttg
     2161 cgccggaggt cgtcgttggg acgccgcgcg gcatcacccc cgagaagccg atcgcctcgc
     2221 gaggaggatc cctcgctgcg agaagaacgt gtcaccgagc acgcccccgc catgaatgac
     2281 tcgcgaaccc tcctgcgctg cgccctggtg ctggccggcg ccctcgccgc ttcggccgcg
     2341 ctcgcgcagg ccccggccgc cgcaccctcg gcctccgcc
//
LOCUS       DASVLN010000857         7202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_155982, whole genome shotgun sequence.
ACCESSION   DASVLN010000857 DASVLN010000000
VERSION     DASVLN010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155982"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VF453_20155"
     CDS             complement(<1..425)
                     /locus_tag="VF453_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690031.1"
                     /translation="MRVRLLQLSASLLLASLVACASRVERGAAMPVAPMDGSDRPAAT
                     PSARVEAPHVPEWLRIRLGDYDAQAGAGAPRAVYALDYRGETAFLVQAGCCDQLDPLI
                     DARGVLICHPTGGYTGKGDGKCPAPLPPADRRREVWRHR"
     gene            complement(526..1602)
                     /gene="holA"
                     /locus_tag="VF453_20160"
     CDS             complement(526..1602)
                     /gene="holA"
                     /locus_tag="VF453_20160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX7690032.1"
                     /translation="MQVRPEQLADHLAKGLRDVYVIHGDEPLRAQEAADAIRAAARAA
                     GCGERKVFVASGAHFDWSGVLGASQAMGLLADQQLIEIRIPSGKPGKDGGEALQQLAR
                     EAGPDSRLLVHLPRLDWQQQKSAWFAALEGAGVAIAADVIERNALPAWLAKRFAAQGQ
                     RVEPGAAGEATLAFMADRVEGNLLAAHQEIQKLGLLHPPGELTFAQVEEAVLDVARHD
                     VSRLCDAVMAVQVGRALRMLDGLEAEGEAAVFVHFKLSDDIRAIKRIQDAQAEGKPLP
                     MAMREARIWGARERLFERAVPRVTPAMATRLVGAASECDGVLKGLRHPQWPVEPWDAL
                     RRLVLMMLQALRPEGVRAGLVLRT"
     gene            complement(1622..2332)
                     /gene="lptE"
                     /locus_tag="VF453_20165"
     CDS             complement(1622..2332)
                     /gene="lptE"
                     /locus_tag="VF453_20165"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX7690033.1"
                     /translation="MSADPSSAAAVRSPARRGLARAFAALAALALAALAGCGFHLRVP
                     QKLNYERIALTGFADRSTMAEEIKRALPPDARIVATVAESQVVIEAVEDTRNTTVEAS
                     TAFGQVRELELHVKLRYRVLRPDGTELLPVADVERFRDLTYDESQALAKDTELSVLYR
                     DLQSDMATQVLRVLAAVGKPGGPAAQVGAALAASAATSGQPASGSRAASPPAPYQPAS
                     AGMWGAAPPSPLPSPSTH"
     gene            complement(2339..4987)
                     /gene="leuS"
                     /locus_tag="VF453_20170"
     CDS             complement(2339..4987)
                     /gene="leuS"
                     /locus_tag="VF453_20170"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7690034.1"
                     /translation="MNEKYVPSEVESAAQAAWQAADAYRVTEDSARAKFYACSMLPYP
                     SGKLHMGHVRNYTINDMLTRQLRMKGMNVLMPMGWDAFGLPAENAAIKNRVPPAKWTY
                     DNIAHMKGQMQAMGLAIDWSREVATCDPAYYKWNQWLFLKMLEAGICERRTQIVNWDP
                     VDQTVLANEQVIDGHGWRSGAPVEKREIPGYYLKITQYAEELLSAVADPEDPDYLAGW
                     PERVRLMQENWIGKSEGVRFAFPHAIHDDDGRLIGDGKMYVFTTRADTIMGVTFCAVA
                     PEHPLAAHAARGNPQLAAFIEQCKAGGTTEAELATKDKEGLPTGLTVVHPLTGAAVPV
                     WVGNYVLMSYGDGAVMGVPAHDERDFAFAKKYGLPIKQVVAVPGETFSTDAWADWYGD
                     KQRGSCMDSGALDGFGYKAAVDRVAELLAAAGLGEKKTTWRLRDWGISRQRYWGTPIP
                     IIHCDGCGVVPVPEKDLPVRLPEDLIPDGSGNPLNKHAAFLNVACPTCGKPARRETDT
                     MDTFVDSSWYFMRYCDPKRDDAMVAEGTDYWMPMDQYIGGIEHAILHLLYARFWTKVM
                     RDLKLVKVSEPFTKLLTQGMVLKGAYFHKPADAGKHYYWEEEIDVERDAHGNVLSAKL
                     KADGTPLVYEMTTMSKSKNNGVDPQALIDRYGADTARLFVMFASPPEQTLEWNDAGVE
                     GAHRFLRRVWGFGAKHEALLRGAPASLAGLEFGPAAKALRRDVHLVLKQVSYDYERMQ
                     YNTVVSGAMKLLNTLEAFADDGTPGSAAALREGFSVLLRALYPACPHIGHALWTSLGY
                     ADVEGPLLDAKWPAVDDGALVQDEVELVLQVAGKTRGSVRVPAAADKAAIEAIALASP
                     EAQRWMEGRPAKKVVVVPGRLVNIVA"
     gene            complement(5350..5784)
                     /locus_tag="VF453_20175"
     CDS             complement(5350..5784)
                     /locus_tag="VF453_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7690035.1"
                     /translation="MAFGRLERTPASQPMSDINMTPLIDVMLVLLVIFMIAAPLMTSS
                     LKMDLPKTAGAQPNEGGPSVTVGLTADGRLHVGEDTVTQSDLVRTLQVAAKRQPQPDV
                     LLRADAAVPYGRVAQLIGLVQDAGLTHVGFVAQPQPGAAPAK"
     gene            complement(5826..6521)
                     /locus_tag="VF453_20180"
     CDS             complement(5826..6521)
                     /locus_tag="VF453_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7690036.1"
                     /translation="MGDPGGLAGFWGESDWISRSVAVLLFAMSVSSWVIIVWKGWLLR
                     RAGRDIQLGVAAFWGAASVGDARARLQAIDREAVLLPLLDAALLRPGAGTLETQPALA
                     SQLTRRLRDALHAGLAQLQFGQVVLASIGSTAPFIGLFGTVWGIFHALAGMADGGGMT
                     LDKIAGPVGEALIFTAAGLAVAIPAVLAYNVFGKRVSACEADLEGFAHDLREMQLDTA
                     GGLARSRPAGDDA"
     gene            complement(6521..>7202)
                     /gene="dapB"
                     /locus_tag="VF453_20185"
     CDS             complement(6521..>7202)
                     /gene="dapB"
                     /locus_tag="VF453_20185"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX7690037.1"
                     /translation="LGQDAAAFLGRTSGVALTADLDAALAGARVLIDFTRPEGTMRHV
                     AACAQRGVALVIGTTGLTDAQKDEIRAASARTPIFLAPNMSVGVNLVMRLIEQAAKSL
                     AEGYDVEIIEAHHRNKVDAPSGTALHMGEVVAKATGRTLSECAVYGREGVTGVRDPGT
                     IGFATVRGGDIIGDHTVLFAGIGERIEISHKSSSRATYAQGSLRAARHLATAAPGLYD
                     MNDVLGLR"
BASE COUNT          936 a         2575 c         2651 g         1040 t
ORIGIN      
        1 cagcgatgcc gccagacctc gcgccgacgg tcggccggcg gcagcggcgc cgggcacttg
       61 ccgtcgccct tgcccgtgta gccgccggtc ggatggcaga tgagcacgcc gcgcgcgtcg
      121 atcagcggat cgagctggtc gcagcagccc gcctgcacga ggaaggccgt ctcgccgcga
      181 tagtcgagcg catagaccgc gcgcggcgcg ccggcgccgg cctgcgcgtc gtagtcgccc
      241 aggcggatgc gcagccactc gggcacgtgc ggcgcctcga cccgggcgga cggcgtggcg
      301 gcggggcggt cggatccgtc catcggcgcg acgggcatgg cggcaccacg ctcgacgcgg
      361 gaggcgcagg cgacgagcga cgcaaggagc aggctggccg acagctgcag taagcggacg
      421 cgcatgatgg gtctcccgga cgacgccgcc gtcgggcaat cggcgtgctc gtcctggccg
      481 gtcagcgcgg ccaggcgccg gccggtccgt cgcggggccc aggtttcagg tccgcagcac
      541 cagccccgcc cgcacgccct cgggccgcag cgcctgcagc atcatcagca cgagccggcg
      601 caacgcatcc cagggttcga cgggccattg cggatgccgc aggcccttga gcaccccgtc
      661 gcactcgctc gcggcgccga ccaggcgcgt ggccatcgcg ggcgtgacgc ggggcacggc
      721 gcgttcgaac aggcgctcgc gcgcgcccca gatgcgcgcc tcgcgcatcg ccatcggcag
      781 cggcttgccc tcggcctgcg cgtcctggat gcgcttgatg gcccggatgt cgtccgagag
      841 cttgaagtgc acgaagacgg ccgcctcgcc ctcggcctcg aggccgtcga gcatgcgcag
      901 cgcccgcccg acctgcacgg ccatcacggc atcgcacagc cgcgagacgt cgtggcgcgc
      961 gacgtcgagc acggcttcct cgacctgcgc gaaggtgagc tcgccgggcg gatgcagcag
     1021 gccgagcttc tggatctcct ggtgcgcggc gagcagattg ccctcgacgc ggtcggccat
     1081 gaaggcgagc gtcgcctcgc cggccgcacc gggctcgacg cgctggccct gtgccgcgaa
     1141 gcgcttcgcg agccaggccg gcagcgcgtt gcgctcgatg acgtcggcgg cgatcgcgac
     1201 gccggcaccc tccagcgccg cgaaccatgc ggatttctgc tgctgccagt ccaggcgcgg
     1261 caggtgcacc agcagccgac tgtccggccc ggcctcgcgc gcgagctgct gcagcgcctc
     1321 gccgccgtcc ttgccgggct tgcccgaggg gatgcggatc tcgatcagct gctggtcggc
     1381 gagcaggccc atcgcctgcg aggcgccgag cacgccgctc cagtcgaagt gcgcgccgct
     1441 cgcgacgaag accttgcgct cgccgcaacc ggccgcccgt gccgcggcgc ggatcgcgtc
     1501 ggcggcctcc tgcgcgcgca gcggctcgtc gccgtggatc acgtagacgt cgcgcaatcc
     1561 cttggcgagg tggtcggcaa gctgctccgg gcggacctgc atggcgggcg tccggcggtg
     1621 gtcagtgcgt cgacggcgat ggcagcggcg acggcggcgc cgcgccccac atgcccgccg
     1681 aggccggttg gtaaggcgcc ggcggggacg cggcgcgcga cccggacgca ggctggcccg
     1741 acgtcgctgc agacgccgcc agcgccgccc cgacctgcgc agccgggccg ccgggcttgc
     1801 cgacggccgc cagcacgcgc agcacctgcg tggccatgtc ggactgcagg tcgcggtaga
     1861 gcacgctcag ctcggtgtcc ttggcgagcg cctgggattc gtcgtaggtg aggtcgcgga
     1921 aacgctcgac gtcggccacc ggcagcagtt ccgtgccgtc gggccgcagc acgcggtacc
     1981 gcagcttcac gtgcagctcg agctcgcgca cctggccgaa ggcggtggag gcctcgaccg
     2041 tcgtgttgcg ggtgtcctcg accgcctcga tgacgacctg ggactcggcc accgtcgcga
     2101 cgatgcgggc gtcgggcggc agcgcgcgct tgatctcctc ggccatggtc gagcggtcgg
     2161 cgaagccggt gagcgcgatg cgctcgtagt tcagcttctg cggcacgcgc aggtggaagc
     2221 cgcatccggc cagcgccgcc agggcgagcg cggccagcgc ggcgaaggcg cgcgcgaggc
     2281 cgcggcgggc cggcgaccgg acggcggcgg cggagctggg atcggcgctc acgacgggtc
     2341 aggccacgat gttgaccaga cggcccggca cgacgacgac cttcttcgcc ggcctgcctt
     2401 ccatccagcg ctgggcctcg ggcgaggcga gcgcgatcgc ctcgatcgcc gccttgtccg
     2461 cggcagcggg cacgcgcacg ctgccgcgcg tcttgccggc gacctgcagc acgagctcga
     2521 cctcgtcctg caccagcgcc ccgtcgtcga ccgccggcca cttcgcatcg agcagcgggc
     2581 cctcgacatc cgcgtagccg agcgaggtcc acagcgcgtg cccgatgtgc gggcaggcgg
     2641 ggtacagcgc gcgcagcagc accgagaagc cctcgcgcag cgccgccgcc gagcccggcg
     2701 tgccgtcgtc ggcgaaggcc tccagcgtgt tgagcagctt catcgcgccg gagacgacgg
     2761 tgttgtactg catgcgctcg tagtcgtagc tgacctgctt gagcaccagg tgcacgtccc
     2821 ggcgcagcgc cttggcggcc gggccgaatt cgaggccggc cagggacgcg ggcgcgccgc
     2881 gcagcagcgc ctcgtgcttc gcgccgaagc cccagacgcg gcgcaggaag cggtgcgctc
     2941 cctcgacgcc ggcgtcgttc cactcgagcg tctgctcggg cggggacgcg aacatcacga
     3001 acaggcgcgc cgtgtccgcg ccgtagcggt cgatcagggc ctgcgggtcg acgccgttgt
     3061 tcttcgactt cgacatcgtc gtcatctcgt agaccagcgg cgtgccgtcg gccttgagct
     3121 tggcgctcag cacgttgccg tgggcatcgc gctcgacgtc gatctcctct tcccagtagt
     3181 agtgcttgcc cgcgtcggcc ggcttgtgga agtacgcgcc cttgagcacc atgccctgcg
     3241 tcagcagctt ggtgaacggc tcggagacct tcacgagctt caggtcgcgc atcaccttcg
     3301 tccagaagcg tgcgtacagc aggtgcagga tcgcatgctc gatgccgccg atgtactggt
     3361 ccatcggcat ccagtagtcg gtgccctcgg cgaccatcgc gtcgtcgcgc ttcgggtcgc
     3421 agtagcgcat gaagtaccac gacgagtcca cgaaggtgtc catcgtgtcc gtctcgcggc
     3481 gcgccggctt gccgcaggtc gggcaggcga cgttcaggaa ggccgcgtgc ttgttcagcg
     3541 ggttgccgga gccgtcgggg atcaggtcct cgggcaggcg caccggcagg tccttttcgg
     3601 gcacgggcac gacgccgcac ccgtcgcagt ggatgatggg gatcggcgtg ccccagtagc
     3661 gctggcggct gatgccccag tcgcgcaggc gccaggtcgt cttcttctcg ccgaggccgg
     3721 cggcggccag cagctcggcg acgcggtcga ccgcggcctt gtagccgaag ccgtccaggg
     3781 cccccgagtc catgcagctg ccgcgctgct tgtcgccgta ccagtcggcc caggcgtcgg
     3841 tggagaaggt ctcgccgggg acggccacga cctgcttgat cggcaggccg tacttcttcg
     3901 cgaaggcgaa gtcgcgctcg tcgtgcgccg gcacgcccat cacggcgccg tcgccgtagc
     3961 tcatgagcac gtagttgccg acccacaccg gcaccgcggc gccggtcagc gggtggacaa
     4021 cggtcaggcc ggtgggcagg ccctccttgt ccttggtcgc gagctcggcc tcggtcgtgc
     4081 cgccggcctt gcactgctcg atgaaggcgg ccagctgcgg gttgcctcgg gcggcgtgcg
     4141 cggcgagcgg atgctccggc gcgacggcgc agaaggtcac gcccatgatc gtgtcggcgc
     4201 gggtggtgaa gacgtacatc ttcccgtcgc cgatcaggcg gccgtcatcg tcgtggatcg
     4261 cgtgcgggaa ggcgaagcgc acgccctcgc tcttgccgat ccagttctcc tgcatcaggc
     4321 gcacgcgctc gggccagccg gcgaggtagt cgggatcctc gggatcggcc accgccgaca
     4381 gcagttcctc ggcgtactgc gtgatcttca ggtagtagcc ggggatctcg cgcttctcga
     4441 ccggcgcacc cgagcgccag ccgtggccgt cgatcacctg ctcgttggcg agcacggtct
     4501 ggtcgaccgg gtcccagttg acgatctgcg tgcggcgctc gcagatgccc gcctcgagca
     4561 tcttcaggaa cagccactgg ttccacttgt agtacgccgg gtcgcaggtg gcgacctcgc
     4621 gcgaccagtc gatcgccagg cccatcgcct gcatctggcc cttcatgtgg gcgatgttgt
     4681 cgtacgtcca cttcgcgggc ggcacgcggt tcttgatggc ggcgttctcg gccggcaggc
     4741 cgaaggcgtc ccagcccatg ggcatcagca cgttcatgcc cttcatgcgc agctggcgcg
     4801 tgagcatgtc gttgatcgtg tagttgcgca cgtggcccat gtgcagcttg ccgctggggt
     4861 agggcagcat cgagcacgca tagaacttcg cgcgcgcgct gtcttcggtg acgcggtagg
     4921 catcggcggc ctgccaggcg gcctgggcgg cggattcgac ctcggagggg acgtacttct
     4981 cgttcattcc ccgattgtag gaggcgctcg ccgccggccc gccgcgcgga accctggatt
     5041 ttcggccgtt tcgacgccga ggcttcccgg cgagccgctg caaaatggtc gcgggcccgg
     5101 cctttcgccg ggatcggagg gagcgatggc cgtcgtgcgc ggccatcccc gttcgcgagg
     5161 acgacgcgct gcgcggctac gccatggccg gtgcggcgcg tggtgcgcgg acggcatccc
     5221 tgcgattcgc gcgacgcgcc ggaggtcacg gcgcgacggg ccgggccctt cctccgcagg
     5281 cggccgccga gcgcggcatc ccgagattca ggccgtacgg gcgcgtgcgg cccgcggcgc
     5341 gaggcctcgt cacttcgccg gtgcggcccc cggctgcggc tgtgcgacga atcccacgtg
     5401 cgtgagcccc gcgtcctgca cgaggccgat gagctgcgcc acgcggccgt agggcacggc
     5461 ggcgtcggcg cgcagcagca cgtcgggctg cggctggcgc ttcgcggcca cctggagcgt
     5521 gcgcacgagg tcggactgcg tgaccgtgtc ctcgccgacg tgcagccggc cgtcggcggt
     5581 caggcccacc gtgacggacg ggccgccctc gttgggctgc gccccggcag tcttcggcag
     5641 gtccatcttc aggctggagg tcatcagcgg cgcggcgatc atgaagatca ccagcagcac
     5701 cagcatgacg tcgatcagcg gcgtcatgtt gatgtcgctc atcggctgcg acgccggggt
     5761 gcgctcgagg cgtccgaagg ccatggcggg tgggcgtggc tgtgggcgtg gcgccggcgc
     5821 gcggctcagg cgtcgtcgcc cgcggggcgg ctgcgggcga ggccaccggc ggtgtcgagc
     5881 tgcatctcgc gcaggtcgtg ggcgaagcct tcgaggtcgg cctcgcacgc cgacacccgc
     5941 ttgccgaaca cgttgtaagc gagcaccgcc ggaatcgcca ccgccaggcc ggcggcagtg
     6001 aagatcagcg cctcgccgac cgggccggcg atcttgtcga gcgtcatgcc gccgccgtcg
     6061 gccatgcccg cgagcgcatg gaagatgccc cagacggtgc cgaacaggcc gatgaacggc
     6121 gcggtggagc cgatcgaggc cagcaccacc tggccgaact gcagctgcgc gagcccggcg
     6181 tgcagcgcgt cgcgcaggcg gcgcgtgagc tgcgaggcga gcgcgggctg cgtctcgagc
     6241 gtccccgcgc cgggacgcag cagcgcggcg tcgagcaggg gcaacagcac ggcctcgcgg
     6301 tcgatcgcct gcaggcgcgc gcgcgcgtcg cccacgctgg ccgcgcccca gaaggcggcg
     6361 acgcccagct ggatgtcgcg tcccgcgcgg cgcagcagcc agcccttcca gacgatgatg
     6421 acccagctgc tgacggacat cgcgaacagc agcacggcga cgctgcggct gatccagtcg
     6481 ctctcgcccc agaagcccgc gagcccgccc ggatcgccca tcagcgcagt cccaggacgt
     6541 cgttcatgtc gtagagcccg ggcgcggcgg tcgcgaggtg gcgcgcggcg cgcaggctgc
     6601 cctgggcgta ggtcgcgcgg ctggagctct tgtggctgat ctcgatgcgc tcgccgatgc
     6661 cggcgaacag cacggtgtgg tcgccgatga tgtcgccgcc gcggacggtc gcgaacccga
     6721 tcgtgcccgg gtcgcgcacg ccggtgacgc cctcgcggcc gtagacggcg cattccgaca
     6781 gggtgcggcc cgtcgccttg gccacgacct cgcccatgtg cagcgcggtg ccgctgggcg
     6841 cgtcgacctt gttgcggtgg tgggcctcga tgatctcgac gtcgtagccc tcggcgagcg
     6901 acttcgcggc ctgctcgatc aggcgcatca cgaggttcac cccgacgctc atgttgggcg
     6961 cgaggaagat cggcgtgcgc gccgacgcgg cgcggatctc gtccttctgc gcgtccgtca
     7021 ggcccgtcgt tccgatcacc agcgcgacgc cacgctgggc gcaggctgcg acgtggcgca
     7081 tcgtcccctc ggggcgcgtg aagtcgatca gcacgcgcgc gccggcgagc gccgcgtcga
     7141 ggtcggccgt cagcgcgacg ccgctcgtgc ggccgaggaa ggccgcggcg tcctggccca
     7201 gc
//
LOCUS       DASVLN010000858         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_367156, whole genome shotgun sequence.
ACCESSION   DASVLN010000858 DASVLN010000000
VERSION     DASVLN010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367156"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..>2072)
                     /locus_tag="VF453_20190"
     CDS             complement(83..>2072)
                     /locus_tag="VF453_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEX7690038.1"
                     /translation="AERAAARAGMATKLQRLAPADVDAAALPVVLLLKDDKACVLIGR
                     EPGRARVLLPETGQGTVLLDDAVLAERSTGLVLSVRPHFRFDARTAGTTPKPAGHWFW
                     STVLGQRKVYRDILWAAALINVFALAMPMFTRNVYDRVVPNAAVETLWALSIGIVIVL
                     GSDLLMRTLRSHFVDEASARIDVQISASLLERVLGMRLENRPASVGSFSQNLRGFEQV
                     RDVITSTTVTALIDLPFALLFLVVIACISPWLAVPVVAAFAGVLAYGWSVQKKLHALA
                     ETTYRASALRNSTLVECLTGIETIKSQGAESVIQARWERTNVHLAATNVKMRGLSSSS
                     IYVTSTVSQLMSVALVIVGVYLIGARELSMGALVAVSMLAGRAMAPAGQIVGLMMQYQ
                     GARTALAGLEKIMAQDTERGPDVSYVERRDLEGALEFRNVAFHYPNRRENALEGVSFK
                     IKPGEKVALIGRVGSGKSTIQRLAMGLYQPTAGAVLVDGIDLRQVDPADLRRNMGYVS
                     QDVLLFYGTLRENIAFGMPYAEDGAIVAAAEVAGLLDTVNRHPQGFDMPVGERGESLS
                     GGQRQSVGIARAVLHNAPILLLDEPTSAMDFSSEAAITQKLMNFARTKTVVLVTHRTS
                     LLALVDRVIVIDGGKVVADGPRDRIMEALQSGRIARAQ"
BASE COUNT          300 a          735 c          738 g          299 t
ORIGIN      
        1 gtccttgtcc tcgacggtgc tcatcgggcg acccccctcc tgcggacttc ggggcgggcg
       61 gcttcgggcg gcggggcgaa gctcattgcg ccctcgcgat ccgcccgctc tggagcgctt
      121 ccatgatgcg gtcgcgcggg ccgtccgcga ccaccttgcc gccatcgatg acgatcacgc
      181 ggtcgacgag cgcgagcagc gacgtgcggt gcgtgacgag gacgacggtc ttcgtgcgcg
      241 cgaagttcat cagcttctgc gtgatggccg cctcggacga gaagtccatc gcgctggtcg
      301 gctcgtcgag cagcaggatc ggcgcgttgt gcagcacggc gcgggcgatg cccacgctct
      361 ggcgctggcc gcccgagagc gactcgccgc gctcgcccac cggcatgtcg aagccttgcg
      421 ggtggcggtt gacggtgtcg agcagcccgg cgacctccgc ggccgcgacg atggcgccgt
      481 cctcggcgta gggcatgccg aaggcgatgt tctcgcgcag cgtgccgtag aacagcagca
      541 cgtcctgcga gacgtagccc atgttgcggc gcaggtcggc cgggtcgacc tggcgcaggt
      601 cgatgccgtc gacgagcacg gcgcccgcgg tcggctggta caggcccatc gccagccgct
      661 ggatcgtcga cttgcccgag cccacgcgcc cgatcagcgc caccttctcg ccgggcttga
      721 tcttgaagct cacgccctcg agcgcgttct cccgccggtt cgggtagtgg aaggcgacat
      781 tgcggaactc cagcgcgccc tcgaggtcgc gccgctcgac gtacgagacg tccggcccgc
      841 gctcggtgtc ctgcgccatg atcttctcga gcccggccag cgcggtgcgc gcgccctggt
      901 actgcatcat caggccgacg atctggcccg ccggcgccat cgcgcgcccg gccagcatgc
      961 tgaccgccac cagcgcgccc atgctgagct cgcgcgcccc gatcaggtag acccccacga
     1021 tcacgagcgc gacgctcatc agctgcgaga cggtcgaggt cacgtagatc gacgacgacg
     1081 acagcccccg catcttcacg ttggtcgccg ccaggtgcac gttggtgcgc tcccagcgcg
     1141 cctggatcac gctctcggcg ccctgggact tgatcgtctc gatgccggtc aggcattcga
     1201 ccagcgtcga gttgcgcagg gcgctggcgc ggtacgtggt ctcggccagc gcgtgcagct
     1261 tcttctgcac cgaccagccg taggcgagca cgccggcgaa agccgccacg accggcaccg
     1321 cgagccaggg cgagatgcag gcgatcacga ccaggaacag cagcgcgaac ggcaggtcga
     1381 tcagcgcggt gacggtggtc gaggtgatga cgtcgcgcac ctgctcgaag ccgcgcaggt
     1441 tctgcgagaa cgatccgacc gacgccggcc ggttctccag gcgcatgccc agcacccgct
     1501 cgagcaggct ggcggagatc tggacgtcga tgcgcgcgct cgcctcgtcg acgaagtggc
     1561 tgcgcagcgt gcgcatcagc aggtcgctgc cgagcacgat gacgatgccg atggacaggg
     1621 cccacagcgt ctcgaccgcg gcgttgggca cgacgcggtc gtagacgttg cgggtgaaca
     1681 tcggcatcgc cagcgcgaag acgttgatca gcgcggcggc ccacaggatg tcgcgataga
     1741 ccttgcgctg gcccagcacc gtgctccaga accagtggcc ggcgggcttg ggcgtcgtcc
     1801 cggcagtgcg ggcgtcgaag cggaagtgcg gccgcaccga cagcaccagc cccgtcgagc
     1861 gttccgccag cacggcgtcg tcgagcagca cggtgccctg gccggtctcg ggcagcagca
     1921 cgcgggcgcg gccgggctcg cggccgatca gcacgcaggc cttgtcgtcc ttgagcagca
     1981 gcacgaccgg cagcgcggcg gcgtcgacgt cggcgggggc gaggcgctgc agcttggtcg
     2041 ccatgcccgc gcgcgcggcg gcgcgctcgg cc
//
LOCUS       DASVLN010000859        15640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_420875, whole genome shotgun sequence.
ACCESSION   DASVLN010000859 DASVLN010000000
VERSION     DASVLN010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15640
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420875"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="lplT"
                     /locus_tag="VF453_20195"
     CDS             complement(<1..791)
                     /gene="lplT"
                     /locus_tag="VF453_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEX7690039.1"
                     /translation="MPDVFLLLLAAQFFSALADNALLLVTIALLQAHGMPAWWAPMLK
                     VGFIASYVVLAPFVGPLADAFPKARLMAWMNGLKMLGVVGLVAGTHPLIAFAIVGLGA
                     AAYAPAKYGLATELVRPDQLVAANGWIEVAAVCAVLLGTGLGGVLVSPWMLALAGAGD
                     AVTHGTLCPSLGVVLAVYLLAGALNLAVRDTGARHAVSSLHPGRLMREFVQANRCLWS
                     DRDGGLSLAVTTIFWGVGATLQFAVLRWASERVHLSLDRAAFLQAA"
     gene            1241..2347
                     /locus_tag="VF453_20200"
     CDS             1241..2347
                     /locus_tag="VF453_20200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7690040.1"
                     /translation="MSAQSRPLTIVHAVFSSRLAGGERHCVDLARAQAALGHDVHVVG
                     PGKSAVRQVLGPTVTYHGLPAPLLRGLRLRRLARTLKADIVHGHLGPACKAVATVGTA
                     TRIGTLHVGFKAHHHQAMDGLICVNTAQRQRLAGYAGQHAVIFNWAPERDPGAAAAPS
                     DLRAELGLGPQQLLVGSVGRLCPSKGMDVLVSAFRAHAPADAVLAILGEGDHMAELRR
                     LAAGDPRVRLLGFRSDVDDALKAMDLFVSPSREEAFPLAILEAMRAGVPVISTATQGP
                     LEMLARQPDARIVPIADVTAMGKAIAERLAGLRAVPRHLRAAIPYEVSAYSRGAAVDR
                     TVAFYRQVLARAPLGEHQANAGVSSDVRPGVGHA"
     gene            2347..3270
                     /locus_tag="VF453_20205"
     CDS             2347..3270
                     /locus_tag="VF453_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690041.1"
                     /translation="MLKRPRTDFWTVGIIPAPIAAIAADPARLRALRGDIAWMPDAGP
                     WRYFADPFALARDGTLHVFVEAFDYRTKHAVIERHEFGLADRRWRGGRVVLARPFHLS
                     YPNVFEHAGETYMLPESAHAGEVALYRAVDGLDRWERAHVLLPQVRGADASVIEHGAR
                     WWTFYTIIGPGGRDQRELHAAHAPALEGPWTPLSANPIRVDRSGARPAGRPFLDAEGR
                     VMLPVQDCSGGYGSALRLLRFDELSPERVRCSPTATRLTGDLASDDHVAGLHTLTACG
                     EFTLIDVKRVDRSRMKQWLDLKRRARRLVGA"
     gene            complement(3351..4016)
                     /locus_tag="VF453_20210"
     CDS             complement(3351..4016)
                     /locus_tag="VF453_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690042.1"
                     /translation="MSSRKLGATLRSRAKIGLIFGEDKNDADALARLAEALWPDAPRF
                     KYVRKPLVLIRSRQEAEDRKRNAAGIADAIRAEGVLHDVAVVIAHRDCDAVEPAHELQ
                     AHTIRSELENAGVKNVIPAAPAWETEAWWFLWPDAVAAVHSKWTRLKRVGNHGMIKDA
                     AEQLARDLRNKSTRREYEKSDAGAIANNVRNFGLIDRCTGTSASFLDFRRRVRVVAGL
                     EIE"
     gene            complement(4013..5221)
                     /locus_tag="VF453_20215"
     CDS             complement(4013..5221)
                     /locus_tag="VF453_20215"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7690043.1"
                     /translation="MRSLLSITAKNFLSLQDVEVELQELSVLVGPNGAGKSNLLKVIQ
                     FLGDTARYDLGPAIQEHGGYERLVFRSPSKEHRAPRITLGIKGRFTKNSSDIAPDEYT
                     LSFSQRQGNAAAHNLVFSQRREEFIFKRKAGPGRRITIAGGKAEIQRATQQRQLALAT
                     TSSGLSTLPKLGRDEGGEQITMLADLLKNFRVFEVDAKKARRPAEFDRENSKLLDSDA
                     GNLASFLRWLHDYESDVFELFVDDLRTIAPTVEDVRVVKIGGGSDEAVAVQLKERGLA
                     GLTNMSEASFGTIRAMALLAMLHDPNPPRLTCVEEIDHGLHPHALDRIVERLRSASRR
                     TQLLVVTHSPALANRLNPGELIVCERDLETGASRIPAISSINVARMAAVSNLSLGELW
                     FSGALGGGLE"
     gene            complement(5618..5694)
                     /locus_tag="VF453_20220"
     tRNA            complement(5618..5694)
                     /locus_tag="VF453_20220"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5658..5660),aa:Val,seq:gac)
     gene            complement(5729..7060)
                     /locus_tag="VF453_20225"
     CDS             complement(5729..7060)
                     /locus_tag="VF453_20225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7690044.1"
                     /translation="MARSSSSALPSQEIGPGPIEAAHRIMQRLLSPFDGSLALRLWDG
                     VTHTFGKAAPAFTLVFRDPTVLRDLVLRRSPLPLAEAYFHGRLDVEGDLYAALRLKTY
                     FQGLTFSGRERAGLLVDALRLAFMPKRAALVGEVRETTQFRHDHSLDSDRAAISHHYD
                     VSNDFYKLFLDERMVYSCAYFHEDDDTIEAAQAQKLDHICRKLRLKPGGRHLDIGCGW
                     GALVIWAAKHYGVKAHGITLSKEQLAEAQRRIAAEGLQDRVTVELRDYRKLEGQGVYD
                     SVSSVGMFEHVGLANLPEYHATVHRVLKPNGLFLNHGITHEEKGWERNVDTEFINTYV
                     FPDGELDRVSSIQAGMEDAGFEIHDVENLRQHYARTLRHWVQRLEANHEEALRHVAEA
                     TFRVWRLYMAACAIEFESGGTGIHQILASRRPDRRPYAQPVPLTRRDLYNA"
     gene            complement(7283..9574)
                     /locus_tag="VF453_20230"
     CDS             complement(7283..9574)
                     /locus_tag="VF453_20230"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX7690045.1"
                     /translation="MKTLATPAVAEDAPAIVLVADADMARGATGSGEGDARSPADPDA
                     SGAIALVRARAFAEPLLIGRRLDSGEDAWPHAQGVAQILADLGATPAMQAAAWLVYAG
                     DGLQRPAEVVEKAFGPSYASVVELTRKLVRIQRNAREARIDEDKRALQTERVRKMLLA
                     FSRDLRVVLLRLASRLQTLRWHAASKTPCPPELAQESAQVFAPLANRLGIWQIKWELE
                     DLSFRFLQPDEYRRIAKLLDERRVEREAGIEAFRAQLAAMLGEAGIRADIQGRPKHIY
                     SIWKKMRGKALDFDHVFDVRAVRVIVDDVADCYAVLGRVNERFRAVDGEFDDYIARPK
                     PNGYQSLHTVVKDDAGRAFEIQIRTREMHEHAEHGVAAHWAYKEAGTKGYAGVVAAGA
                     FEEQVAQARKAVLRQLLAWERDFVAEEASAAASPDDRIYVFTPQASVIELPAGGTPVD
                     FAYALHTDVGHRCRGARVDGAMVPLSTPLKNGQTVEITTVREGGPSMDWLNPELHFLA
                     SPRARAKVRAWFNARQHEQTVAKGRELVEKLLQREGRTAISLEELAGRLGFDDAQALF
                     EVVGKDEYSLRNIENLLRPAEPAPADEAIPLRRPRSGGATSRGGVLVVGIDSLLTTLA
                     SCCRPAPPDAIGGYVTRGKGVAVHRADCTNFRHMAETNPERVIPVEWGQRAQGADAKA
                     GVYAVDIVVEAQDRQGLLRDISEVLAKQRVNVIGVNSQSTRGGADRRALMTFTIEIDG
                     AERLGQVLQLVGRVGGVEAARRR"
     gene            complement(9571..10455)
                     /locus_tag="VF453_20235"
     CDS             complement(9571..10455)
                     /locus_tag="VF453_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7690046.1"
                     /translation="MSEPRLRHVQCLSPEGLHRLAYWEWGDAANPRVLVCAHGLTRQG
                     RDFDTLARAMAGEFRVVCPDVAGRGRSSWLKNPASYAIPQYVADMVTLLARLDACELS
                     WVGTSMGGLIGMGLAGFEDSPVARLVLNDVGPTIEYAALQRIGTYVGQPVRWRSLEEA
                     AESLRQISLGFGPHTREQWLELTRPQAVVDPDGDGTVLKPHYDPAIAAPFKAITPELA
                     AAGEALLWQTYDRLRCPTLVLRGAESDLLSSATARQMSERGPRARVHEFAGVGHAPTL
                     VQAEQVAVVREFLLAGSP"
     gene            complement(10580..11320)
                     /locus_tag="VF453_20240"
     CDS             complement(10580..11320)
                     /locus_tag="VF453_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550486.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX7690047.1"
                     /translation="MTRPHSPRRLVARLVVPFALAAFAAGAGAASPSGAAPEPEGVLE
                     LSASATVQVPNDWITVEFSATRDGTDANAVQAALKQALGAALELARREAKPEGHVEVE
                     GGGFSLQPRFDAKGRVDGWTGSTSMRVQGRDMATIAALAGRIATMNVSRLDYGVSREA
                     REKVEGEVAAQAIARFRAQAQDYAKAFGYGGFVVRDVNIQTQGDGPVPPRPLMAARMA
                     VASVEAVLPTAAGTGSVTANVNGSIQMK"
     gene            11496..12230
                     /gene="ompR"
                     /locus_tag="VF453_20245"
     CDS             11496..12230
                     /gene="ompR"
                     /locus_tag="VF453_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HEX7690048.1"
                     /translation="MSAVPGTRQDRIVVVDDDARIRDLLRRYLSQEGFDVLLAEDGKA
                     LNRLMTRENIDLIVLDLMLPGEDGLSICRRLRAAGDRTPIIMLTAKVEDVDRIVGLEV
                     GADDYLPKPFNPRELLARVHAVLRRRPPQEAPGAPAKDAQVVQFGDFEFDLSLRRLTK
                     DGEPIALTTGEFSMLKALVRHPRQPLSRDKLAQLARGRDFEPFDRSLDVQISRLRKMI
                     EPDPSNPRYIQTVWGVGYVFVPSETA"
     gene            12257..13591
                     /locus_tag="VF453_20250"
     CDS             12257..13591
                     /locus_tag="VF453_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7690049.1"
                     /translation="MARKLLSLNLFWRTFFLLAVLLTGGILAWVQTLRQLEFEPRAVI
                     AAQQIASLVNLSRGAMAHVDGINRIALMKSMTDQEAVRVLPREPKDRWEPYDVDAFTR
                     RIAAELRIRLGDGTIVARSVNGQEALWVGFTIDDDRYWLQAEPQRVTPLTSSTWFVWV
                     GIAILATVIGSAMIAGLINRPLKELSTAAARIRDGEYDTRLDERTSTTEVRAVNVGFN
                     RMARELAKVDQDRTVMLAGISHDLRTPLARIRLEAEISIPDEDARSHIAEDIDQLDAI
                     IDKFMDYARPGVTRLEPVPLAAVIEREAIAFRDTSQIRIALQLSPVSRVLGDPTELGR
                     VFGNLFENARRYGRTSESEPAEVTVGQIQAGPWIIVTVRDRGPGVPPDKLTQLTTPFF
                     RGDTARTAATGAGLGLAIVKKAIQRMGGSLEMTNGAEGGLVAHVRLRRVETA"
     gene            13704..14786
                     /locus_tag="VF453_20255"
     CDS             13704..14786
                     /locus_tag="VF453_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin family protein"
                     /protein_id="HEX7690050.1"
                     /translation="MKTIIKATVAAAALCAAGYASADITFYEHQNFYGRQFTVQQPIG
                     DFASAGFNDRASSVVVTDHPWEVCDLPNFHGSCVILRPGNYPSLSSMNLNDRISSARQ
                     VGERRPPPEAWAPAPLPGQVTFYEYPNFRGRSFNTQGEVRDFGEYGFNDRASSVTVIG
                     DRWEVCDDVAWHGHCVVLRPGNYPDLRAMGLDNRISSVRILPPNVPVAQGRWAPPAPP
                     AYDWRPRPSEQLFQANVSSSRAVYGTPTQHCWVDREAVPVDRPRSQVGSAIVGGVIGS
                     ILGHEIAHSSGGAIAGAIGGAAVGNAIGATAQPYQDVQHCSQAVQGPPLYWDTTYWFR
                     GVEHHVQTTAPAGATITVNGYGEPRM"
     gene            complement(14988..>15640)
                     /locus_tag="VF453_20260"
     CDS             complement(14988..>15640)
                     /locus_tag="VF453_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX7690051.1"
                     /translation="RTRGDLLSATGASAAPGGTPAVAGGWREVLAFWRPMRHALAGYL
                     LFGVGYIGYMTFIVSLLREQGVAAGWIAGFYALLGLCTVASSWLWAGVLQRFRGGQAL
                     ALLNALLAVATAMPVAGSNVLAIGLSCALFGATFLSVVASTTAFVRHNLPPAGWARGI
                     AAFTIVFAAGQIIGPSLTGLLADGAGGLARGFAWSAGTLAAGAWIASRQRPLAGRG"
BASE COUNT         2428 a         5666 c         5240 g         2306 t
ORIGIN      
        1 gcggcctgca ggaaggccgc gcggtcgagc gacaggtgca cgcgctccga ggcccagcgc
       61 agcaccgcga actgcagcgt cgcgcccacg ccccagaaga tcgtcgtcac cgcgagcgac
      121 aggccgccgt cgcgatcgct ccaaaggcag cggttcgcct gcacgaactc gcgcatcagg
      181 cggcccggat ggagcgatga aaccgcatgc ctggcgccgg tgtcgcgcac ggcgaggttg
      241 agcgcgcccg ccagcaggta cacggcgagc acgacgccca gcgacgggca cagcgtgcca
      301 tgggtgaccg cgtcgccggc gcccgcgagc gcgagcatcc acgggctcac gagcaccccg
      361 cccaggccgg tgcccagcag caccgcgcac accgccgcga cctcgatcca gccgttggcc
      421 gcgacgagct ggtccggccg cacgagctcg gtcgcgaggc cgtacttggc cggcgcgtag
      481 gccgccgcgc cgagccccac gatcgcgaag gcgatcagcg gatgcgtgcc cgccacgaga
      541 ccgacgacgc ccagcatctt caggccgttc atccacgcca tcaggcgcgc cttcgggaac
      601 gcgtcggcca gcggcccgac gaagggtgcg agcacgacgt acgaggcgat gaagccgacc
      661 ttgagcatcg gcgcccacca ggccggcatg ccatgcgcct gcagcagcgc gatggtcacg
      721 agcagcagcg cgttgtcggc gagcgcggag aagaactggg ccgcgagcag gagcaggaac
      781 acatcgggca tcaggcggtc tggcgtgcgt ggaggttcat cgtccccatg aggccggaaa
      841 cgtcactgga ccgcgagcat ggcgccgtcc cgtgaatcgg cggtgacgcc cgcgtgacgg
      901 ccacgcgcca gcctggcgac ggcagcgggc gcgcgacgtc atcgtggcgt catcggcgat
      961 gccccgtgcc gcgcacgccg cacgagccgg ccgtcacgcg accgccacgc gaccgccacg
     1021 cgagcgacat cacggggcga cccggcatcg catcgtcgag aactccccac gccacggtcg
     1081 cgcaccgacc cgctaaggtg cttgtcgcga cgctggccgc ccgctgaacg agcgccttcg
     1141 ccccggaacc gatcccgccg cacgcactcc gtgcatagac ggctgctcga caggttcatg
     1201 acgtaaagtt gtgttaacag gccttttcag agtcgacgac atgtccgccc aatcccgccc
     1261 cctgacgatc gtgcatgccg tcttctcctc gcgcctggcc ggcggcgaac gccactgcgt
     1321 cgacctcgcg cgcgcgcagg ccgcgctggg ccacgacgtc cacgtggtcg ggccgggcaa
     1381 gtcggccgtg cgccaggtgc tggggccgac cgtcacgtac cacggcctgc ctgcgccgct
     1441 gctgcgcggg ctgcgcctgc gccgcctcgc gcgcacgttg aaggccgaca tcgtgcacgg
     1501 gcacctcggg ccggcctgca aggcggtcgc gacggtgggc accgcgacgc gcatcggcac
     1561 gctgcacgtc ggcttcaagg cgcatcacca ccaggcgatg gacggcctca tctgcgtcaa
     1621 caccgcgcag cgccagcgcc tggccggcta cgcgggccag cacgccgtca tcttcaactg
     1681 ggcgcccgag cgcgacccgg gcgccgcagc cgccccgagc gacctgcgcg ccgagctcgg
     1741 cctcggcccg cagcagctgc tggtcggctc cgtcgggcgc ctgtgcccga gcaagggcat
     1801 ggacgtgctc gtgtcggcgt tccgtgcgca cgcgccggcg gacgcggtgc tcgccatcct
     1861 cggcgagggc gaccacatgg ccgagctgcg ccgcctcgcc gccggcgacc cgcgcgtgcg
     1921 gctgctgggc ttccgcagcg acgtcgacga tgcgctcaag gcgatggacc tgttcgtctc
     1981 gccgtcgcgc gaggaggcct tcccgctcgc gatcctcgag gcgatgcgcg cgggcgtgcc
     2041 ggtgatctcg accgccacgc agggcccgct ggagatgctc gcccgccagc ccgacgcccg
     2101 gatcgtcccg atcgccgacg tcaccgcgat gggcaaggcc atcgccgagc gcctggcggg
     2161 gctgcgcgcc gtgccacgcc acctgcgtgc cgcgatcccg tacgaggtgt cggcgtactc
     2221 gcgcggcgcg gccgtcgatc gcaccgtcgc gttctaccgc caggtgctcg cgcgcgcgcc
     2281 gctgggcgag caccaggcga atgcgggcgt gtcgtccgac gtccgcccgg gcgtgggcca
     2341 cgcctgatgc tgaagcggcc gcgaaccgac ttctggaccg tcgggatcat tcccgccccg
     2401 atcgccgcga tcgccgccga tccggcgcgc ctgcgcgcgc tgcgcgggga catcgcctgg
     2461 atgcccgacg ccggtccgtg gcgctatttc gccgacccgt tcgcgctcgc gcgcgacggc
     2521 acgctgcacg tcttcgtcga ggccttcgac taccgcacca agcacgccgt catcgagcgc
     2581 cacgagttcg ggctcgccga ccggcgctgg cgcggcgggc gcgtggtgct cgcgcgaccg
     2641 ttccacctgt cgtacccgaa cgtgttcgaa cacgcgggcg agacctacat gctgcccgag
     2701 agcgcccacg ccggcgaggt cgcgctgtac cgcgcggtcg acggcctcga ccgctgggag
     2761 cgcgcgcacg tgctgctgcc gcaggtgcgc ggcgccgacg cctcggtgat cgagcacggc
     2821 gcccgctggt ggacgttcta caccatcatc ggcccgggcg gccgcgacca gcgcgaactg
     2881 cacgccgccc acgccccggc cctggaaggc ccgtggacgc cgctgtccgc caacccgatc
     2941 cgcgtcgacc gcagcggcgc ccgcccggcc ggccggccct tcctcgacgc cgagggccgc
     3001 gtgatgctgc cggtgcagga ctgctccggc ggctacggca gcgcactgcg cctgctgcgc
     3061 ttcgacgaac tctcgcccga gcgcgtgcgc tgctcgccca ccgcgacgcg cctgaccggc
     3121 gacctcgcct ccgacgacca cgtggccggc ctgcacaccc tcaccgcctg cggcgagttc
     3181 acgctgatcg acgtcaagcg ggtcgaccgc tcgcgcatga agcagtggct cgacctgaag
     3241 cggcgcgcgc gtcggctcgt gggtgcttga acactgcgcg acttccaact tttcaacgcc
     3301 ctgtcgtcgt caagaagcgt atgtgtcgta ggcagcctct ttcaaagccg ttactcgatt
     3361 tcaaggccgg cgacaacgcg cacgcgacgc ctaaaatcca agaacgaggc acttgttcct
     3421 gtgcaccgat caatcaaacc aaaattacga acattgttcg caattgctcc ggcatcactc
     3481 ttttcgtatt cgcggcgggt cgacttgttg cgaaggtccc gagccaattg ttcagcggcg
     3541 tccttaatca tgccgtgatt accgacacgc ttcagccttg tccattttga atggacggcc
     3601 gcaacggcgt ctggccaaag gaaccaccag gcctccgtct cccaagctgg agcggctgga
     3661 atcacatttt ttacgccggc attctcaagc tcacttcgga tcgtgtgagc ctgcaattcg
     3721 tgcgcaggct ctacagcgtc acaatctcga tgggctatca ctacggcaac atcgtgaagc
     3781 acgccctcgg ctcgaatagc gtcagctatg cccgcagcgt tccgctttcg atcctcagcc
     3841 tcttgccgac tcctaattaa aacaagaggc tttcgcacat atttgaatcg aggcgcatcc
     3901 ggccacagcg cctcggccag ccgagccagt gcgtccgcgt cgtttttatc ctctccaaag
     3961 atcagaccga tcttcgccct cgacctcagc gtggctccaa gctttcggga actcattcca
     4021 gccccccgcc aagcgcgcct gaaaaccaaa gctcacccaa gctaagattg gaaaccgccg
     4081 ccatccttgc aacgttaata gacgagattg caggaatccg cgaggcaccg gtttcaagat
     4141 cgcgctcaca cacaatcaac tccccgggat tgaggcgatt cgccagagca ggagaatgtg
     4201 taacaaccag caactgcgtt cggcgcgaag cacttcgcaa gcgttcgacg atccgatcga
     4261 gagcatgcgg atgcaatccg tggtcgatct cctccacgca agtcaatcgt ggtgggttcg
     4321 gatcatgaag cattgcaaga agcgccatcg ccctgatcgt tccaaatgag gcctcggaca
     4381 tattggtaag cccggccagc ccccgctcct ttagctgaac tgcgacagct tcgtcacttc
     4441 cgcctccaat tttgactacc cgcacgtctt caacggttgg agctatggtt cgaagatcgt
     4501 cgacaaacaa ttcaaagaca tcagattcgt aatcatgaag ccagcggaga aagctcgcca
     4561 agttcccggc atcgctatct agtaatttcg aattttctcg atcaaattcc gctggacggc
     4621 gagccttttt cgcatcgacc tcgaagacgc ggaagttctt aagcaaatcc gctagcattg
     4681 ttatctgctc accaccttca tctcgaccca acttcggaag cgtagagaga ccagacgacg
     4741 ttgtagctag cgccaattgc cgttgctgag ttgcgcgttg aatttcggcc ttgcctccag
     4801 caattgtgat gcggcgacca gggcccgcct tccgcttgaa gataaactct tctcgtcgct
     4861 ggctgaaaac caagttgtgc gcggccgcat ttccttgacg ctgcgagaaa ctaagcgtgt
     4921 actcatccgg cgcaatgtca gatgaatttt tagtaaatct gcccttaatg cccaacgtga
     4981 tacggggtgc tctatgttcc tttgaaggac tacgaaacac cagacgctcg taaccgccgt
     5041 gctcctggat tgctggccca agatcgtagc gagccgtatc gccgagaaac tgaatcacct
     5101 tgagcagatt cgatttccct gccccatttg gcccaaccaa aaccgacaac tcctgaagtt
     5161 ctacctcaac gtcctgcagg cttaggaagt tcttcgcggt gattgaaagg agggaacgca
     5221 tacgatggcg ataagggtgc cgttccggcg aatgctgcac tgtatctcgc atcaaggcca
     5281 caaaactaac ttcgggccag cttttttgta gagccgcgac ggaatacgag acgcttccgc
     5341 gccaccaaat ccgttgcgaa tgtgggggga gggtcagcag atttcgacga agtatgtagc
     5401 cataagaccg gagggctcgg gatcgtctct ccttcgcgga acatggcggc gcaagccacc
     5461 gaagccaaga gttccgccag gcctgttcgc atcggaccga tttgactggt cgcagcagtc
     5521 gtccgcgcat gccttccgag ccacgagcca tcgctaccaa tggactaact gtaaagcgaa
     5581 aaggccgatc ccctttcgga aatcggcctt tggcatttgg taggcgcgat tggactcgaa
     5641 ccaacgaccc ccaccatgtc aaggtggtgc tctaaccagc tgagctacgc gcctgcaatc
     5701 aagcaagact gacagtctag catgcttttc aggcgttgta caagtcccgt cgcgtcaggg
     5761 gaaccggctg cgcgtaggga cggcgatccg ggcggcgcga ggcgaggatc tggtggatgc
     5821 ccgtgccgcc cgattcgaac tcgatcgcgc aggccgccat gtacaggcgc cagacgcgga
     5881 aggtggcctc ggcgacgtgg cgcagcgctt cctcgtgatt ggcttccagg cgctggaccc
     5941 agtggcgcag cgtgcgggcg tagtgctggc gcaggttctc gacgtcgtgg atctcgaagc
     6001 ccgcgtcctc catgccggcc tggatcgagc tgacgcgatc gagctcgccg tccgggaaca
     6061 cgtaggtgtt gatgaactcc gtgtcgacgt tgcgctccca gcccttttcc tcgtgcgtga
     6121 tgccgtgatt caggaacagg ccgttcggct tgagcacgcg gtgcacggtg gcgtggtatt
     6181 cgggcaggtt ggcgaggccc acgtgctcga acatgccgac gctggagacg ctgtcgtaga
     6241 cgccctgccc ctcgagcttg cggtagtcgc gcagttcgac ggtcacccgg tcctgcaggc
     6301 cctccgccgc gatgcggcgc tgcgcctcgg cgagttgctc cttgctcagc gtgatgccgt
     6361 gcgccttcac gccgtaatgc ttggccgccc agatcaccag cgcgccccag ccgcagccga
     6421 tgtcgaggtg gcggccgccc ggcttcaggc gcagcttgcg gcagatgtgg tcgagctttt
     6481 gcgcctgcgc cgcttcgatg gtgtcgtcgt cctcgtggaa gtaggcacag gagtagacca
     6541 tgcgctcgtc gaggaacagc ttgtagaagt cgttcgagac gtcgtagtgg tgcgagatcg
     6601 ccgcgcggtc gctgtcgagc gagtggtcgt ggcggaactg cgtggtctcg cggacctcgc
     6661 cgacgagcgc agccctcttc ggcatgaacg cgaggcgcag cgcgtcgacg agcaggcccg
     6721 cgcgctcgcg gccgctgaac gtgaggccct ggaagtaggt cttcaggcgc agcgcggcgt
     6781 agaggtctcc ctcgacgtcg agccggccgt ggaagtaggc ctccgccagc ggcagcgggc
     6841 tgcgccgcag gacgaggtcg cgcagcaccg tcgggtcgcg gaacaccagc gtgaaggccg
     6901 gcgccgcctt gccgaaggtg tgggtgacgc cgtcccacag gcgcagcgcg aggctgccgt
     6961 cgaacgggct cagcagccgc tgcatgatgc ggtgggcggc ctcgatgggc ccggggccga
     7021 tctcttgact gggcagggcc gacgacgatg aacgagccat gggatctccg gacaggcggc
     7081 gagcgctgcg cgggaggtgc gcttgcagga tctcgcggcc gcctcgcgcg gcgcgagccc
     7141 cgtcgcgccg ccggcgcccg tgcccggctc gccaatgcag gaccatgcta tcgcgcgtgc
     7201 gcgtgcccgc aaaggcgtac ggatttgtcc aaaggcatcg cggccccgcg gtcctggcaa
     7261 acccggaccg ccgtccgccg cctcagcgcc ggcgcgcggc ctcgacgccg ccgacgcggc
     7321 cgaccagctg cagcacctgc cccagccgct cggcgccgtc gatctcgatc gtgaacgtca
     7381 tgagcgcgcg ccggtcggcg ccgccgcgcg tggactgcga gttgacgccg atcacgttga
     7441 cgcgctgctt ggcgagcacc tcggagatgt cgcgcagcag gccctggcga tcctgcgcct
     7501 cgacgacgat gtcgaccgcg tagacgcccg ccttcgcgtc cgcgccctgc gcgcgctggc
     7561 cccactcgac cgggatcacc cgctcggggt tggtctcggc catgtggcgg aagttcgtgc
     7621 agtccgcgcg gtgcaccgcc acccccttgc cgcgcgtcac gtagccgccg atcgcgtccg
     7681 gcggcgccgg gcggcagcag ctcgcgagcg tcgtcagcag cgagtcgatg cccaccacca
     7741 gcacgccgcc gcggctggtc gcgccgcccg agcgtggccg ccgcagcggg atcgcctcgt
     7801 cggcgggcgc gggctcggcc gggcgcagca ggttctcgat gttgcgcagc gaatactcgt
     7861 ccttgcccac gacctcgaag agggcctgcg cgtcatcgaa gccgagccgg ccggccagct
     7921 cctccaggct gatcgcggtg cggccctcgc gctgcaggag cttctcgacc agctcgcgcc
     7981 ccttggcgac ggtctgctcg tgctggcgcg cgttgaacca ggcccgcacc ttggcgcgcg
     8041 cccgcgggct ggcgaggaag tgcagctccg ggttgagcca gtccatcgac gggccgccct
     8101 cgcgcaccgt ggtgatctcc accgtctggc cgttcttcag cggcgtcgac agcggcacca
     8161 tcgcgccgtc gacgcgcgcg ccgcggcagc ggtggccgac gtcggtgtgc agcgcatacg
     8221 cgaagtcgac cggcgtgccg cccgcgggca gctcgatcac gctcgcctgc ggcgtgaaga
     8281 cgtagatgcg gtcgtccggg gacgccgcgg ccgaggcctc ctcggcgacg aagtcgcgct
     8341 cccacgcgag cagctgccgc agcaccgcct tgcgcgcctg cgcgacctgc tcctcgaacg
     8401 cgcccgcggc caccacgccg gcatagccct tggtgccggc ctccttgtac gcccagtgcg
     8461 cggcgacgcc gtgctcggcg tgctcgtgca tctcgcgcgt gcggatctgg atctcgaacg
     8521 cacggcccgc gtcgtccttc acgaccgtgt gcagcgactg gtagccgttg ggcttcggcc
     8581 gcgcgatgta gtcgtcgaac tcgccgtcga ccgcgcggaa gcgttcgttc acgcggccga
     8641 gcacggcgta gcagtcggcg acgtcgtcga cgatcacgcg caccgcgcgc acgtcgaaca
     8701 catggtcgaa gtccagcgcc ttgccgcgca tcttcttcca gatgctgtag atgtgcttgg
     8761 gccggccctg gatgtcggca cggatgcctg cctcgcccag catcgcggcg agctgggcgc
     8821 ggaacgcctc gatgccggcc tcgcgttcga cgcgccgctc gtcgagcagc ttcgcgatgc
     8881 ggcggtactc gtcgggctgg aggaaccgga acgagaggtc ctcgagctcc cacttgatct
     8941 gccagatgcc gagccggttg gcgagcggcg cgaacacctg cgccgactcc tgcgccagct
     9001 ccggcgggca cggcgtcttc gaggccgcgt gccagcgcag cgtctgcagg cgcgaggcca
     9061 ggcgcagcag gacgacgcgc aggtcgcgcg agaacgccag cagcatcttg cgcacgcgtt
     9121 cggtctgcag cgcgcgcttg tcctcgtcga tgcgcgcctc gcgcgcgttg cgctggatgc
     9181 gcacgagctt gcgcgtgagc tcgacgacgc tggcgtacga cggcccgaac gccttctcga
     9241 ccacctcggc cggccgctgc aggccgtcgc ccgcgtagac gagccaggcc gccgcctgca
     9301 tcgcgggggt ggcaccgagg tcggccagga tctgcgcgac gccctgcgcg tgcggccagg
     9361 cgtcctcgcc cgagtcgagc ctgcggccga tcagcagtgg ctccgcgaac gcgcgcgcgc
     9421 gcacgagcgc gatcgcgccc gacgcgtcgg gatcggcggg cgagcgggcg tcgccttcgc
     9481 ccgatccggt cgcgccgcgc gccatgtcgg cgtcggccac cagcacgatc gccggggcgt
     9541 cctcggcgac cgcgggcgtg gcgagcgtct tcatggcgag ccggccagca ggaactcgcg
     9601 cacgacggcc acctgctccg cctgcacgag cgtcggcgcg tggcccacgc cggcgaactc
     9661 gtgcacccgc gcgcgcgggc cgcgctccga catctggcgc gcggtcgcgg acgacagcag
     9721 gtccgattcc gcgccgcgca gcaccagcgt cgggcagcgc agccggtcgt aggtctgcca
     9781 cagcagcgcc tcgccggcgg cggcgagctc gggcgtgatg gccttgaacg gcgcggcgat
     9841 ggcgggatcg tagtgcggct tgaggacggt gccgtcgccg tccgggtcga ccacggcctg
     9901 cgggcgcgtg agctcgagcc actgctcgcg cgtgtgcggc ccgaacccca gcgagatctg
     9961 gcgcagcgac tccgcggcct cctcgagcga ccgccagcgc accggctgtc ccacgtaggt
    10021 gccgatgcgc tgcagcgccg cgtactcgat ggtcgggccg acgtcgttga gcacgaggcg
    10081 cgcgaccggc gaatcctcga acccggccag gcccatcccg atcagcccgc ccatcgaggt
    10141 gccgacccag ctcagctcgc aggcgtccag ccgcgcgagc agcgtgacca tgtcggcgac
    10201 gtactgcggg atcgcgtacg aggccgggtt cttcagccac gaggagcggc cgcggccggc
    10261 gacgtcgggg cagacgacgc ggaactcgcc ggccatggcg cgcgccagcg tgtcgaagtc
    10321 ccggccctga cgcgtcaacc cgtgcgcgca caccagcacg cgcgggttcg cggcgtcgcc
    10381 ccactcccag tacgccagcc ggtgcaggcc ttcgggcgag aggcactgga cgtggcggag
    10441 tcggggttcg gacatggcgg cggctcggga cgtgggcccg cggcggcggg cgatcccggc
    10501 atcgtagcaa gcgccgcgcc gccgccggcg cccgccgccg agaaacccgg cgccacgcat
    10561 cgcggcgggc gcccgccgct cacttcatct ggatggagcc gttgacgttg gcggtgacgg
    10621 agcccgtgcc ggcggcggtc ggcagcaccg cctcgaccga tgccacggcc atgcgcgccg
    10681 ccatcagcgg ccgcggcggc accgggccgt cgccctgcgt ctggatattg acgtcgcgca
    10741 cgacgaagcc gccatagccg aaggccttcg cgtagtcctg cgcctgcgca cggaagcgcg
    10801 cgatcgcctg cgcagcgacc tcgccctcga ccttctcgcg cgcctcgcgc gagacgccgt
    10861 agtcgagccg gctcacgttc atcgtcgcga tgcggccggc gagcgcggcg atcgtggcca
    10921 tgtcgcggcc ctgcacgcgc atggaggtcg agcctgtcca gccgtccacg cggcccttgg
    10981 cgtcgaagcg cggctgcagc gagaaaccgc ccccctccac ctcgacatgg ccctcgggct
    11041 tcgcctctcg gcgggcgagc tccagcgccg cgccaagcgc ctgcttgagc gccgcctgca
    11101 cggcgttggc gtcggtgccg tcccgggtgg ccgagaactc gacggtgatc cagtcgttgg
    11161 gcacctggac cgtggcgctg gccgacagct cgagcacccc ttcgggctcg ggggccgcgc
    11221 cggacgggga tgcggcgccc gcccccgcgg cgaaggcggc gagcgcgaac gggacgacga
    11281 ggcgcgcgac gaggcggcgc ggggaatgcg gacgggtcat ggggatccct ggtcggtcga
    11341 acatgcgagt tgaacgcggg ctttgcttca gcggggtgac gggtgcggtg tccccgtggc
    11401 aagatttgta acagaaggtc aggtgcggcc cgaaatcgcc gaagccgcgg tccggcagcc
    11461 cccagaatct cgcttgttac atatttggag atgccatgtc ggccgttcca ggcacccgcc
    11521 aagaccgcat cgtcgtcgtc gacgacgacg cccgcatccg cgacctgctg cgtcgctacc
    11581 tgagccagga aggcttcgac gtcctgctcg ccgaggacgg caaggcgctc aaccggctga
    11641 tgacccgcga gaacatcgac ctgatcgtgc tcgatctcat gctccccggc gaggacggcc
    11701 tgtcgatctg ccgccgcctg cgcgccgccg gcgaccgcac ccccatcatc atgctgaccg
    11761 ccaaggtcga ggatgtcgac cgcatcgtcg gcctcgaggt gggcgcggac gactacctgc
    11821 ccaagccctt caacccgcgc gagctgctcg cccgcgtgca tgccgtcctg cgccgccgcc
    11881 cgccgcagga ggcgccgggc gcgccggcca aggacgccca ggtcgtgcag ttcggcgact
    11941 tcgagttcga cctctcgctg cgccgcctca ccaaggacgg cgagcccatc gcgctgacga
    12001 cgggcgagtt ctcgatgctc aaggcgctcg tgcgccatcc gcgccagccg ctgtcgcgcg
    12061 acaagctcgc ccagctcgcg cgcggccgcg acttcgagcc cttcgaccgc agcctcgacg
    12121 tgcagatctc gcgcctgcgc aagatgatcg agcccgaccc gtccaacccg cgctacatcc
    12181 agacggtctg gggcgtcggc tacgtgttcg tgcccagcga gacggcgtga cgccgcggcg
    12241 ccgaggcctc tgagggatgg cccgcaagct cctgtcgctg aacctgttct ggcgcacgtt
    12301 cttcctgctg gcggtgctgc tgacgggcgg catcctcgcc tgggtgcaga cgctgcgcca
    12361 gctcgagttc gagccgcgcg cggtcatcgc ggcgcagcag atcgcgagcc tcgtgaacct
    12421 ctcgcgcggc gcgatggcgc acgtcgacgg catcaaccgc atcgcgctga tgaagtcgat
    12481 gaccgaccag gaggcggttc gcgtgctccc gcgcgagccc aaggatcgct gggagcccta
    12541 cgacgtcgat gccttcaccc ggcgcatcgc cgcggagctg cgcatccgcc tgggcgacgg
    12601 cacgatcgtc gcgcgctcgg tcaacggcca ggaggcgctg tgggtcggct tcacgatcga
    12661 cgacgaccgc tactggctgc aggccgagcc gcagcgcgtc acgccgctga cctccagcac
    12721 gtggttcgtg tgggtgggca tcgcgatcct cgcgacggtc atcggctcgg cgatgatcgc
    12781 gggcctcatc aaccggccgc tcaaggagct gtccaccgcg gccgcccgca tccgcgacgg
    12841 cgagtacgac acccggctcg acgagcgcac cagcacgacg gaggtgcgcg cggtcaacgt
    12901 cggcttcaac cgcatggcgc gcgagctcgc gaaggtggac caggatcgca ccgtcatgct
    12961 ggccggcatc tcgcacgacc tgcgcacgcc gctcgcgcgc atccgcctcg aggccgagat
    13021 cagcatcccc gacgaggacg cacgcagcca catcgccgag gacatcgacc agctcgacgc
    13081 gatcatcgac aagttcatgg actacgcgcg accgggcgtc acgcgcctgg agccggtgcc
    13141 gctggcggcg gtgatcgagc gcgaggcgat cgccttccgc gacacgagcc agatccgcat
    13201 cgcgctgcag ctgtcgccgg tctcgcgcgt gctcggggat ccgaccgagc tcgggcgcgt
    13261 gttcggcaac ctgttcgaga acgcgcgccg ctacggccgc acgagcgagt ccgagccggc
    13321 cgaggtcacc gtcggccaga tccaggccgg cccctggatc atcgtgaccg tgcgcgaccg
    13381 cggccccggc gtgccgcccg acaagctcac gcagctgacg acgcccttct tccgcggcga
    13441 caccgcccgc accgcggcga ccggggccgg gctgggcctg gccatcgtca agaaggcgat
    13501 ccagcgcatg ggcggcagcc tcgagatgac caacggcgcc gagggcgggc tggtcgctca
    13561 cgttcgttta cgtcgcgtcg aaacagcttg atgcgaactt gactccgcgg cggtcaacgc
    13621 gcgccttcgc gcggccgttc tgggatgacg ggcgcctcga ttgcctgacg ggcgccgtcc
    13681 ccgacaacaa aaggagtctc gcgatgaaga ccatcatcaa ggccacggtg gccgcggcgg
    13741 cactgtgcgc ggcgggctac gccagcgccg acatcacgtt ctacgagcac cagaacttct
    13801 acggccggca gttcacggtc cagcagccga tcggcgactt cgccagcgcc ggcttcaacg
    13861 accgcgcctc gtcggtcgtc gtcacggacc atccctggga agtctgcgac ctgcccaact
    13921 tccacggcag ctgcgtcatc ctgcggcctg gcaactaccc gtcgctcagc tcgatgaacc
    13981 tgaacgaccg catctcctcg gcgcgccaag tcggcgagcg ccggccgccg ccggaggcct
    14041 gggcccccgc gccgctgccc gggcaggtga ccttctacga atacccgaac ttccgcggcc
    14101 gctcgttcaa cacgcagggc gaggtccggg acttcggcga atacggcttc aacgaccgtg
    14161 cgtcctccgt gaccgtcatc ggcgaccgct gggaggtctg cgacgacgtc gcctggcacg
    14221 gccactgcgt cgtgctgcgc ccgggcaact accccgacct gcgcgcgatg ggcctggaca
    14281 accgcatctc gtcggtgcgc atcctgccgc ccaacgtgcc ggtcgcccag ggccgctggg
    14341 cgccgcccgc gccgccggcc tacgactggc gcccgcggcc gtcggagcag ctgttccagg
    14401 ccaacgtgag ctcctcgcgg gccgtctacg ggacgccgac gcagcactgc tgggtcgacc
    14461 gcgaggccgt gcccgtcgac cgcccgcgca gccaggtcgg cagcgcgatc gtcggcggcg
    14521 tgatcggctc catcctcggc cacgagatcg cgcactccag cggcggcgcg atcgccggcg
    14581 cgatcggcgg cgcggccgtc ggcaacgcga tcggcgcgac ggcgcagccg taccaggacg
    14641 tccagcactg ctcgcaagcc gtccagggcc cgccgctgta ctgggacacg acctactggt
    14701 tccgcggcgt ggaacaccac gtgcagacca ccgccccggc cggcgcgacc atcaccgtca
    14761 acggctacgg cgagccgcgc atgtgaggca agcgccccgc gcgcgagcga cgcgcgggtg
    14821 gggcgcccac ccgcctgcga ccaaaccccc cggcgggcgg ccttcgcgcg agcgcccgtc
    14881 atcggccgga catccgcgcg gacgacagtg gtcgggccag ctgacaacca caaacccgcg
    14941 cacgaacccc tgaacccgga agcctccggg ttcttttttt ccgctggcta gccgcggcct
    15001 gccagcggcc gctggcgcga cgcgatccag gcgcccgccg ccagcgtccc cgcggaccac
    15061 gcgaacccgc gcgcgagccc gcctgcgccg tcggccagga gcccggtgag gctgggcccg
    15121 atgatctgcc ccgccgcgaa cacgatggtg aaggccgcga tgccgcgggc ccagccggcc
    15181 ggcggcaggt tgtggcgcac gaaggcggtg gtcgaggcga cgaccgacag gaaggtcgcg
    15241 ccgaacagcg cgcacgacag cccgatcgcc aggacgttcg atcccgccac gggcatcgcc
    15301 gtcgcgacgg cgagcagcgc gttcagcagc gccagcgcct ggccaccgcg gaagcgctgc
    15361 agcacgcccg cccacagcca ggacgacgcg accgtgcaca gcccgagcag cgcatagaac
    15421 ccggcgatcc agcccgcggc gacgccctgc tcgcgcaaca gcgacacgat gaaggtcatg
    15481 tagccgatgt agccgacgcc gaacagcagg tagcccgcga gggcgtggcg catcgggcgc
    15541 cagaacgcga gcacctcgcg ccatccgccg gcgaccgccg gcgtgccgcc cggtgccgcg
    15601 gacgctcctg tggccgagag caggtcgccc cgcgtccgca
//
LOCUS       DASVLN010000860         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_530404, whole genome shotgun sequence.
ACCESSION   DASVLN010000860 DASVLN010000000
VERSION     DASVLN010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530404"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="VF453_20265"
     CDS             <1..2066
                     /locus_tag="VF453_20265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7690052.1"
                     /translation="ASDAAALDVLLEPSPLPLHYPRFDRIRDADFLPALRVAMARNRQ
                     AAQAVAADSAAPTFDNTILPLERAAAAFARVRLVFENLDASNTNPVLQATQRAVAPEI
                     AAMDDAIHLDPKLFARVDALWRRREALGLDPESARLLWRYHQDFVRAGALLPDDAKAR
                     LRALNVRISQLQTTFAQNTLKERGASAVVFDARAELDGLSDAEIAAAADAAKAAGKPG
                     RFLVELVNTTGQPVLAELRLHASRERVMAASLARGSRGGAYDNRETVIELVRARARKA
                     ALLGYPSFAAFQLGEQTVGSIDTLDGILAKLAPEAEARMRREAAELQGLVDAEHGGFE
                     LGAADWPYYEARLRASRFSVDPDRLKPYYELDHVILDGVFYAANRLYGLTFKERHDLP
                     VYEPSVRVFDVFDQDGKPLAIFIGDYYARPNKNGGAWMNSYVPQDGLDGGRPVVVNNL
                     NIPRPPAGQPTLLTQDEVITAFHEFGHALHGMLSQTRYPRFAGPNVPRDFVEYPSQFN
                     EMWATDPEVLRHFARDWRTGATIPQALVDEMAAAARFDQGFQTTEYLAATLLDQAWHQ
                     TPEARLPTVDGFVAFEEDALRKAGVDYAPVPPRYRSAYFSHAFQSGYAAGYYSYLWSD
                     VLAADSADWVRRHGGLSRATGDQIRAKLLSRGGAADPLALFRDLTGGPPDLGPLLAKR
                     GLDKP"
BASE COUNT          291 a          792 c          717 g          280 t
ORIGIN      
        1 cggcctcgga cgccgcggcg ctcgacgtgc tgctcgagcc cagcccgctg ccgctgcact
       61 acccgcgctt cgaccgcatc cgcgacgccg acttcctgcc cgccctgcgc gtggcgatgg
      121 cgcgcaaccg gcaggcggcg caagccgtcg ccgccgacag cgccgccccg accttcgaca
      181 acacgatcct gccgctggag cgcgccgccg cggcgttcgc gcgcgtgcgg ctggtcttcg
      241 agaacctcga tgcgtccaac acgaaccccg tgctgcaggc gacgcagcgc gcggtcgcgc
      301 ccgagatcgc ggcgatggac gacgccatcc acctcgaccc gaagctgttc gcgcgcgtgg
      361 acgccctctg gcgccggcgc gaagccctgg gcctcgatcc cgagtccgcg cgtctgctgt
      421 ggcgctacca ccaggacttc gtgcgtgccg gcgcgctgct gcccgacgac gcgaaggcgc
      481 ggctgcgggc gctcaacgtg cgcatctcgc aactgcagac gacgttcgcg cagaacacgc
      541 tgaaggagcg cggcgcgagc gcggtcgtct tcgacgcgcg ggccgagctc gacggcctgt
      601 ccgatgcgga gatcgccgct gcggccgatg ccgcgaaggc cgccggcaag ccgggcaggt
      661 tcctggtcga gctggtgaac acgaccggcc agccggtgct cgccgagctg cgcctgcacg
      721 cctcgcgcga gcgcgtgatg gccgcctcgc tcgcgcgcgg cagccgcggg ggcgcgtacg
      781 acaaccgcga gaccgtcatc gagctcgtgc gcgcccgcgc gcgcaaggcc gcgctgctgg
      841 ggtatccgag cttcgccgct ttccagctcg gggagcagac ggtcggctcg atcgataccc
      901 ttgacggtat tctcgccaag ctcgctcccg aagccgaggc ccggatgcgc cgcgaggccg
      961 ccgagctcca gggcctcgtc gacgccgagc atggcggctt cgagctgggc gcggccgact
     1021 ggccgtacta cgaggccagg ctgcgcgcgt cgcgtttctc ggtcgacccg gaccggctca
     1081 agccgtacta cgagctcgac cacgtcatcc tggacggcgt cttctacgcc gccaaccgcc
     1141 tgtacggcct gacgttcaag gagcgccacg acctgccggt ctacgagccc agcgtgcgag
     1201 tcttcgacgt cttcgaccag gacggcaagc cgctcgcgat cttcatcggc gactactacg
     1261 cgcggccgaa caagaacggc ggcgcgtgga tgaacagcta cgtgccgcag gacggcctcg
     1321 acggcgggcg gccggtcgtc gtcaacaacc tcaacatccc gcggccgccc gcgggccagc
     1381 ccacgctgct cacgcaggac gaggtcatca cggccttcca cgagttcggc cacgcgctgc
     1441 acggcatgct gtcgcagacg cgctacccgc gcttcgccgg ccccaacgtg ccgcgcgact
     1501 tcgtcgagta cccgtcgcag ttcaacgaga tgtgggccac cgaccccgag gtgctgcgcc
     1561 acttcgcgcg cgactggcgc accggcgcga cgatcccgca ggcgctggtc gacgagatgg
     1621 ccgccgcggc gcgcttcgac cagggcttcc agaccaccga gtacctggcc gcgacgctgc
     1681 tggaccaggc ctggcaccag acgcccgagg cccggctgcc gacggtcgac ggcttcgtcg
     1741 cgttcgagga ggacgcgctg cgcaaggcgg gcgtcgacta cgcgcccgtg ccgccgcgct
     1801 atcgcagcgc ctatttctcg cacgcgttcc agtccggata cgcggcgggg tattactcgt
     1861 acctgtggag cgacgtgctc gcggccgaca gcgcggactg ggtacggcgc cacggcggcc
     1921 tgtcgcgcgc gaccggcgac cagatccgcg caaagctgct gtcgcgcggc ggcgcggcag
     1981 atccgctcgc gctgttccgc gacctgaccg gcgggccgcc ggatctcggg ccgctgctgg
     2041 ccaagcgggg cctcgacaag ccctgacccg cgccggttcg
//
LOCUS       DASVLN010000861        10139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_99783, whole genome shotgun sequence.
ACCESSION   DASVLN010000861 DASVLN010000000
VERSION     DASVLN010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99783"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VF453_20270"
     CDS             complement(<1..509)
                     /locus_tag="VF453_20270"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX7690053.1"
                     /translation="MSPSILRNLAAPCTAAVALALVGTTFGALAQTYRVVGPDGRVTY
                     TDRPPTAAQLVPGGGGAATGSEGNALPYQTRQAMGKYPVVLYATKSCAPCDQARQWLR
                     GRGIPFSEYSVTSNADGAALQSRFGDATLPVVTIGSQPVKGFNASELQSYADAAGYPA
                     QAKLAGYAWP"
     gene            complement(763..2175)
                     /locus_tag="VF453_20275"
     CDS             complement(763..2175)
                     /locus_tag="VF453_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7690054.1"
                     /translation="MKRYEALAQEIAESIAHGRLRPGERVPSVREASRSRSVSPSTVF
                     EAYYLLEARGLIEARPRSGYFVRHTANSQQEPESSRPVAKERAVEVSELVFQVLGHAR
                     NRALVPMGSAFPSPLLFPLPKLALATGKALRRLDPWRTVEDLSPGSAALRRQIALRYL
                     GLGCGVDTEELVITNGAMEALNLCLESVTQPGDLVAVESPTFYAALQALERRGLRAVE
                     IATHPRTGIDVAALAAVMERHPVKACWLMPNFQNPLGSLMPDDAKQALVELLARHEVP
                     LIEDDVYGELYFGARKPRPAKAWDRAGWVMHCSSFSKTLAPGYRVGWAAAGRYAGEVA
                     QRKLMSSIAAPLPSQEGLSEYLEHGGYDRHLRQLRATLAQHRHATLRDIARHFPPGTR
                     ATRPEGGYFIWVELPPKIDALELHRRALSQGISLAPGHLFSADQRFKHHVRINFGHPY
                     HEDFHAALEAVGAIARALAA"
     gene            2149..2487
                     /locus_tag="VF453_20280"
     CDS             2149..2487
                     /locus_tag="VF453_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690055.1"
                     /translation="MRQGFIALHVRLIRCFSGESESLISATALAQSRLQAKGWSWNLM
                     PHPLRLEPLSRLPMQRIEEIMRWVPIFFIAFKLLVLGTAIFFSIKSHYDGGKQEQEEE
                     RDRQNRINGS"
     gene            complement(2684..4729)
                     /locus_tag="VF453_20285"
     CDS             complement(2684..4729)
                     /locus_tag="VF453_20285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7690056.1"
                     /translation="MTNPLLAASTGIPPFAEIRPEFVTPAMDELLAQSDAALERVVGP
                     EVPPDFDAMSREFTVATERLSRAWRAVEHLTGVADTPELRAAHAENLPRITEFFTRQG
                     ADERLYAKYRAVAAGADAAGLSSVRRKTLANWLRDFKLGGADLEGEAKARYAAIQARS
                     AELSRQFSEHVLDATDGYALYVSEADLAGVPADAVEVARAAARADGRDGCKLTLHFPS
                     YLPVMQMGRNRALREKLYTAYVTRASEFGPPQRDNTPVMRELLQLRQEEAELLGYRNY
                     AELSLVPKMAGSPEEVIAFLRDLGARARPSAERDLKELREFAAQELGIAQLQGWDVPF
                     ASERLKEARYAFSDEEVRQYFTLPKVLEGLFRIIETLFEVRIVRSSAPAWHESVEFYR
                     IERPAPDGGAPTLVGEFFLDLYARPGKRPGAWMDDARQRWLRADSRTLQTPVAHLVCN
                     FAAPVNGRPALLTHDNVITLFHEFGHGLHHMLTRIEELGVSGISGVEWDAVELPSQFM
                     ENFCWEWDVLQQLTAHVETGATLPRPLFDKMLAAKNFQSGMQTLRQVEFALFDMLIHS
                     QPGAPDRVQQILDDVRQEVAVLIPPPFNRFQHSFSHIFGGGYAAGYYSYTWAEVLSAD
                     AFSAFEEAGVLDLETGRRYRREILEVGGSREALESFTAFRGRAPSIDAMLRHLGIAA"
     gene            complement(4857..5708)
                     /gene="folD"
                     /locus_tag="VF453_20290"
     CDS             complement(4857..5708)
                     /gene="folD"
                     /locus_tag="VF453_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEX7690057.1"
                     /translation="MTARLIDGVALSKTLRGEIAQRAAALTARGHQPGLAVILVGEDP
                     ASATYVKNKVKACGEAGIRSVFEKYDADLTETALLERLAALNADPTVHGILVQMPLPK
                     HIDPHKVIETISTDKDVDGYSVRSAGDLMAGLPGFRPCTPYGCMKLIESTGVPIKGKH
                     AVVIGRSNTVGKPMALLLLQANATVTVTHSATPDIGAHTRQADIVVVATGRRDTLRGD
                     MVKPGAIVIDVGINRDEHGKLCGDADFASVSQVAAHVTPVPGGVGPMTITMLLMNTVE
                     SAERTAG"
     gene            complement(5918..6544)
                     /locus_tag="VF453_20295"
     CDS             complement(5918..6544)
                     /locus_tag="VF453_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7690058.1"
                     /translation="MSLIPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVKCFDSAESFL
                     SRFDPREVACVICDIRMDGMNGLELQDRLIERKSPLPIVFVTGHGDVPMAVSTMKKGA
                     MDFIEKPFKEDDLVSLVERMLDQARSAFSQHQEQASRDALLQRLTTREAQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGAPSGKA"
     gene            complement(6541..9114)
                     /locus_tag="VF453_20300"
     CDS             complement(6541..9114)
                     /locus_tag="VF453_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7690059.1"
                     /translation="MQTAHEPAPPRSPSWTQRLFGRWWRSQSAARQDRFATLGPLVSV
                     LLFLAAIVAAFWTLRSEEIERATDAVRRDTEMAQQQIRSRLSEDQDLMVRLTRDIVGH
                     DVDTDSFFTQTASFAPARPEITQVVWLGPDRKPRATFLGSIFRGASSLRGSSEDPSLP
                     VAGAKTASEAAFVAARNTHLPQYSAPFIDVDGTAVFQVQLPLISHTNFAGTLVAEYSI
                     EALLRHAVPEEVTKRRAIEVLDEHEELLASTITSMPGRPSAVPSIAFEAPLAPASNGL
                     ILRGLGYRTSIGLISNSLFWMVLALSGLTVWMLLGTWRHVRRRAQIQSALVHETNFRR
                     AMENSMLTGMRAMDMEGRITYVNPAFCAMTGFSDNELVGRLPPFPHWPHDRIEENSRL
                     LQQELQGRSPAGGIEVKIMRKDGSVFDARMYVSPLIDAKGVQTGWMTSVTNITEAKRV
                     RDQLSASHERFTTVLEGLDAAVSVLSVQQGQLLFANRSYRLWFGSDAHAHALLAGGID
                     ADAPPPEADDAVDGYGGLPAQELTEAGTSPREVYVESLDKWFDVRARYLQWTDGRLAQ
                     MLIATDITARLRAEQLAQQQTERAHFTSRLIAVGEMASSVAHELNQPLTAISNYCSGM
                     VSRVRNESIEKDDLIAALQKTAKQAERAGHIIHRIRNFVKRREPQRQVAQAREMVDDA
                     VELAGIELRRRNVGIQIYVAQRLPPLLVDPILIEQVLLNLLKNAAEAIDGAQMPLLRR
                     QIELRVVPRYTPDEGGVIEFSVTDKGPGLKEDVLNRLYEAFFSTKVEGMGIGLSLCRS
                     IIESHRGRIRAQNLYNGTTVVGCCFAFTLPVETTHRADAANAVAALEGGEGPDTNATA
                     S"
     gene            9309..>10139
                     /gene="aceE"
                     /locus_tag="VF453_20305"
     CDS             9309..>10139
                     /gene="aceE"
                     /locus_tag="VF453_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747281.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEX7690060.1"
                     /translation="MSAIPQSFPGAAANDSDAQETREWTDALSAVIAAEGRERGHFLI
                     EQLLDHARQHGIDLPFSATTAYVNTIAPEDEERFPGNIDIEERLRAYMRWNAMAMVVR
                     ANRLHPADGGDLGGHLSSFASLATMLGCGFNHFWHAESPDHGGDLLYIQGHSSPGIYA
                     RAYLEGRITEEQLVHFRQEVEGKGLSSYPHPKLMPEFWQFPTVSMGLGPLMAIYQARF
                     LKYLHARGIADTSKRKVWVFCGDGEMDEPESLGAIGLAAREKLDNLIFVVNCNLQRLD
                     G"
BASE COUNT         1500 a         3424 c         3631 g         1584 t
ORIGIN      
        1 ggccaggcgt agccggccag cttcgcctgc gcggggtagc cggcggcgtc ggcgtaggac
       61 tgcagctcgc tcgcgttgaa gcccttgacc ggctggctgc cgatggtcac gaccggcagc
      121 gtcgcgtcgc cgaagcgcga ctgcagggcc gcgccatcgg cgttgctggt gaccgagtac
      181 tcgctgaacg ggatgccgcg gccgcgcagc cactgccggg cctggtcgca cggcgcgcag
      241 ctcttggtcg cgtagagcac gacgggatac ttgcccatcg cctggcgcgt ctggtagggc
      301 agggcgttgc cttcggagcc ggtcgccgct ccgccgccgc cgggcacgag ttgcgcggcc
      361 gtgggaggcc ggtccgtgta ggtcacgcgg ccgtccgggc cgacgacccg gtaggtctgc
      421 gccagggcgc cgaacgtcgt gccgaccagc gccagcgcga cggcggccgt gcacggcgcg
      481 gcgaggttgc ggaggatcga gggactcatc gtgggcttca tcaggtggga tgctcgtctt
      541 ggcgcgacac cgggtggatg acgcgtccct ggcaagacaa cgcgccgcgg gccggcggcg
      601 cggacaggaa tgcagtccgc gacgatacgc gatttccgca gcgacgccgg agcccgacgg
      661 taaccgtttc caccgccgcg ttcaaaaaca ccggaaccgc gcgcgggaaa agggggtgat
      721 catcgaaaac ccgccgttct ggccggccgt gttcgcgcgc tgtcaggccg ccagtgcccg
      781 agcgatggct cccaccgcct cgagcgccgc gtggaagtct tcgtggtacg gatgcccgaa
      841 gttgatccga acatggtgct tgaagcgttg gtcggccgag aacaggtggc ccggcgccag
      901 gctgatgccc tgcgaaaggg cccgccggtg gagttccagc gcgtcgatct tcgggggcag
      961 ttccacccag atgaagtagc cgccttccgg tcgcgtggcc cgcgtgccgg gcggaaagtg
     1021 tctggcaatg tcgcgcagcg tcgcatggcg atgctgcgcc agcgtcgccc gcaactgacg
     1081 caggtgccgg tcgtagccgc cgtgttcgag gtactcgctc aggccctcct gcgaaggcag
     1141 gggcgcggcg atggaactca tcagcttgcg ctgcgcgacc tcgccggcgt agcggcccgc
     1201 cgctgcccag ccgacgcggt agcccggcgc cagcgtcttg gagaaggaac tgcaatgcat
     1261 gacccagccc gcacgatccc acgccttggc cggccggggc ttgcgggcgc cgaaatacag
     1321 ctcgccataa acgtcatcct cgatcagcgg cacctcgtgc ctggcgagca gttcgaccag
     1381 ggcctgcttg gcgtcgtccg gcatcaggct gcccagcgga ttctggaaat tgggcatgag
     1441 ccagcaggcc ttgaccggat gccgttccat gaccgcggcc agcgcggcca cgtcgatgcc
     1501 cgtgcgcgga tgggtagcga tctcgacggc gcgcaggccg cggcgttcca gcgcttgcag
     1561 cgcggcatag aaggtcggtg actcgacggc caccaggtcg ccgggctggg tgacgctctc
     1621 caggcagagg ttgagcgcct ccatcgcacc gttggtgatg accagctcct cggtgtccac
     1681 gccgcagccc aggccgagat agcgcagggc gatctggcgg cgcagcgcgg cgctgccggg
     1741 tgagaggtcc tcgaccgtgc gccaagggtc cagccgacgc agcgccttgc ccgtggcgag
     1801 cgcgagcttg ggcaagggga acagcagtgg gctggggaag gccgaaccca tcggcacgag
     1861 cgcgcgattc ctcgcgtggc ccagcacctg gaagaccagt tcggagacct cgacggcgcg
     1921 ctctttggcc accggcctgg aactctccgg ctcctgttgc gagttcgccg tgtggcggac
     1981 gaagtagccg gaacgcgggc gcgcttcgat caggccgcgc gcttccagca ggtaatacgc
     2041 ctcgaagacg gtggaagggc tcacgctccg gctgcggctc gcctcgcgca ccgaaggtac
     2101 acgctcgccc ggccgcaacc ggccgtgggc gatggactcg gcgatctctt gcgccagggc
     2161 ttcatagcgc ttcatgttcg tctgatccgc tgtttttcgg gcgaatctga gtctttgatc
     2221 tcggcgaccg ctttggcaca gtcacggctg caagcaaagg gatggtcatg gaacctcatg
     2281 ccgcatccgc ttcggcttga accgttgtcc aggctgccga tgcagcgtat cgaggaaatc
     2341 atgaggtggg ttccgatatt ctttattgcg ttcaagttgc tcgtgctggg cacggccatc
     2401 tttttctcca tcaagtccca ttacgacgga ggaaagcagg agcaagaaga agaaagggat
     2461 aggcagaaca ggatcaacgg ttcctgagcg cccgcatgcc gcgttcgaaa acgtcgggcg
     2521 agcgaaggga aacatcgggc gagcgtcccg gagcgcgcgc cgtgcaatcg aaaactgtgc
     2581 ggccggcctg cgggatcgcg gcgccgcatt gcgcgaatgc gcggccgaag tttcaaacga
     2641 cggcgtcgtt gtttcggata ccggacgccg ccgcgccggg cgctcaggcc gcgatgccca
     2701 agtggcgcag catcgcgtcg atgctgggcg cgcggccgcg gaacgcggtg aagctctcga
     2761 gggcttcgcg gctgccgccg acctccagga tctcgcggcg gtagcggcgg ccggtctcga
     2821 ggtcgagcac gccggcctcc tcgaaggcgc tgaatgcgtc ggccgacagc acctccgccc
     2881 aggtgtagct gtagtagccg gccgcatagc cgccgccgaa gatgtgcgag aagctgtgct
     2941 ggaagcggtt gaacggcggc gggatcagga ccgccacctc ctgtcgcacg tcgtccagga
     3001 tctgctgcac ccggtcgggc gcgcccggct gggagtggat cagcatgtcg aacagcgcga
     3061 actcgacctg gcgcagggtc tgcatgccgc tctggaagtt cttggccgcg agcatcttgt
     3121 cgaacagcgg ccgcggcagc gtggcgccgg tctcgacatg ggccgtcagt tgctgcagga
     3181 cgtcccattc ccagcagaag ttctccatga actggctcgg cagctcgacg gcgtcccact
     3241 cgacgcccga gatgccggag acgcccaact cctcgatgcg cgtgagcatg tggtgcaggc
     3301 cgtggccgaa ctcgtggaac agcgtgatca cgttgtcgtg cgtgagcagc gccgggcggc
     3361 cgttgaccgg cgccgcgaag ttgcacacca ggtgcgcgac cggggtctgc agcgtgcgcg
     3421 agtccgcgcg cagccagcgc tggcgcgcat cgtccatcca ggcgccgggg cgcttgccgg
     3481 ggcgggcgta gaggtcgagg aagaactcgc cgaccagcgt cggcgcgccg ccgtcgggag
     3541 ccgggcgctc gatgcggtag aactcgacgc tctcgtgcca ggcgggtgcg ctcgagcgca
     3601 cgatgcgcac ctcgaacagc gtctcgatga tgcggaacag cccctcgagc accttgggca
     3661 gcgtgaagta ctggcgcacc tcctcgtcgc tgaaggcata gcgcgcctcc ttcagtcgct
     3721 cgctcgcgaa cgggacgtcc cagccctgca gctgcgcgat gcccagctcc tgcgcggcga
     3781 actcgcgcag ctccttcagg tcgcgttcgg cgctcgggcg cgcgcgggcg ccgaggtcgc
     3841 gcaggaacgc gatcacctcc tcgggcgagc cggccatctt gggcaccagc gacagctccg
     3901 cgtagttgcg gtagccgagc agctcggcct cctcctggcg cagctgcagc agctcccgca
     3961 tcaccggcgt gttgtcgcgc tgcggcggcc cgaactcgct tgctcgcgtg acgtaggcgg
     4021 tatagagctt ctcgcgcagc gcgcggttgc ggcccatctg catcacgggc aggtagctcg
     4081 ggaaatgcag cgtcagcttg cagccgtcgc ggccgtcggc acgggccgcc gcgcgcgcga
     4141 cctcgacggc gtcggccggc acgcccgcga ggtcggcctc cgagacgtac agggcgtagc
     4201 cgtccgtggc gtcgagcacg tgctccgaga actggcgcga gagctccgcg ctgcgcgcct
     4261 ggatcgcggc gtagcgcgcc ttcgcctcgc cctcgagatc ggcgccgccg agcttgaagt
     4321 cgcgcagcca gttggcgagc gtcttgcggc gcacgctcga gagcccggcc gcgtccgcgc
     4381 ccgcggcgac cgcgcggtac ttggcgtaga ggcgctcgtc cgcgccctgg cgggtgaaga
     4441 actcggtgat gcgcgggagg ttctcggcat gggccgcgcg cagctcggga gtgtcggcga
     4501 cgccggtcag gtgctccacg gcgcgccagg cgcgcgacag gcgctcggtg gcgaccgtga
     4561 actcgcgcga catcgcgtcg aagtccggcg gcacctccgg cccgacgacg cgctcgagcg
     4621 ccgcgtcgga ctgcgcgagc agttcgtcca tcgccggggt gacgaactcg gggcggatct
     4681 cggcgaaggg cgggatgccg gtgctggcgg cgagcagggg attggtcatg gcgtcgggga
     4741 tgcgggcgcg cgcccggaat ggcgatccac cgaatgtacg ccggccggcc cgggatcgac
     4801 ctcgcattcc cggcgggaag acgggcgttt cggcgcgcgg cgcgcccggc cctccttcag
     4861 ccggccgtcc gctcggcgga ctcgaccgtg ttcatcagca gcatcgtgat cgtcatcggc
     4921 ccgacgccgc ccggcacggg ggtgacgtgc gcggcgacct gggagacgct cgcgaagtcg
     4981 gcgtcgccgc acagcttgcc gtgctcgtcg cggttgatgc cgacatcgat gacgatcgcg
     5041 ccgggcttga ccatgtcgcc gcgcagcgtg tcgcggcggc cggtggcgac gacgacgatg
     5101 tcggcctggc gcgtatgggc gccgatgtcg ggcgtcgcgc tgtgcgtgac cgtcaccgtg
     5161 gcgttggcct gcagcagcag cagcgccatc ggcttgccca ccgtgttgct gcggccgatc
     5221 acgaccgcgt gcttgccctt gatgggcacg cccgtgctct cgatcagctt catgcagccg
     5281 tagggcgtgc aggggcggaa gcccggcagc ccggccatca ggtcgccggc gctgcgcacc
     5341 gagtagccat cgacgtcctt gtcggtcgag attgtctcga tgaccttgtg cgggtcgatg
     5401 tgcttcggaa gcggcatctg cacgaggatg ccgtggacgg tcgggtcggc gttcagcgcg
     5461 gccaggcgct cgagcagcgc ggtctcggtc aggtccgcgt cgtacttctc gaacaccgag
     5521 cggatgccgg cttcaccgca ggccttgacc ttgttcttca cgtaggtcgc cgaggccgga
     5581 tcctcgccga ccaggatcac cgcgaggccg ggctggtggc cgcgcgcggt cagcgcggcg
     5641 gcgcgctgcg cgatctcgcc gcgcagggtc ttggagaggg cgacgccgtc gatcagtcgt
     5701 gcggtcatgg cgggatcctt cgaagtaggt cgggaaaatg aaagaggccg catcgcaagc
     5761 ggcctctcat gggctcggat gtctcacggc cggcgggcgc ccggccgccc gaaggcggcc
     5821 agcgtgcgcg aggcgcaaga cggtcccccg gactgtctcg cgtccacgcg cacccccccc
     5881 ggggggcggc gagcggcggc gagcccgggc cgtcgattca agccttgccg gacggggcgc
     5941 cgagcgcgat cttgagcagg tcggccaccg tgttggcgtt gagcttctcc atgatgttgg
     6001 cgcggtgcgc ctccaccgtc ttgatgctga tgcccaggtc gtcggcgatc tgcttgttca
     6061 ggcggccggc gacgatgcgc tcgagcacct gcgcctcgcg cgtggtcagg cgctgcagca
     6121 gcgcgtcgcg gctggcctgt tcctggtgct gcgagaaggc cgagcgcgcc tggtccagca
     6181 tgcgctcgac cagcgagacg aggtcgtcct ccttgaacgg cttctcgatg aagtccatcg
     6241 cgcccttctt catcgtcgag accgccatcg gcacgtcgcc gtggccggtc acgaagacga
     6301 tgggcagcgg gctcttgcgc tcgatcaggc gatcctgcag ctccaggccg ttcatgccgt
     6361 ccatccggat gtcgcagatg acgcaggcga cttcgcgcgg atcgaagcgc gacaggaagg
     6421 attccgccga gtcgaagcac ttgacgcggt agtccttgcc ctccagcagc cattgcagcg
     6481 aatcgcggac cgcctcgtcg tcgtcgacca cgtagacggt acctttcttg gggatcaggc
     6541 tcatgaggca gtggcgttcg tgtccggacc ttcgccgcct tccagcgcgg cgaccgcgtt
     6601 cgcggcatcg gccctgtggg tggtttcgac aggcagggtg aacgcgaagc agcaccccac
     6661 caccgtggtg ccattgtaga ggttctgggc gcggatccgg ccgcggtgcg actcgatgat
     6721 cgagcggcac agcgacaggc cgatgcccat gccttccacc ttggtcgaga agaaggcctc
     6781 gtacaggcga ttgagcacgt cctccttgag gcccgggccc ttgtcggtca cgctgaactc
     6841 gatcacgccg ccttcgtcgg gcgtgtagcg cggcacgacg cgcagctcga tttgccggcg
     6901 cagcagcggc atctgcgcgc cgtcgatcgc ctccgcggcg ttcttcagca ggttgagcag
     6961 cacctgctcg atcaggatcg ggtcgaccag cagcggcggc aggcgctggg cgacgtagat
     7021 ctggatgccc acgttgcgcc ggcgcagctc gatgccggcg agctcgaccg cgtcgtccac
     7081 catctcgcgc gcctgcgcca cctggcgctg cggctcgcgc cgcttcacga agttgcggat
     7141 gcggtggatg atgtggccgg cccgctcggc ctgcttggcc gtcttctgca gcgccgcgat
     7201 caggtcgtcc ttctcgatgg actcgttgcg cacgcgcgag accatgccgc tgcagtagtt
     7261 ggagatcgct gtcagcggct ggttgagctc gtgggcgacc gacgacgcca tttcgccgac
     7321 cgcgatcagg cggctcgtga agtgcgcgcg ctcggtctgc tgctgtgcga gctgctcggc
     7381 gcgcaggcgg gcggtgatgt cggtggcgat cagcatctgc gcgaggcggc cgtcggtcca
     7441 ctgcaggtag cgcgcgcgga cgtcgaacca cttgtccagg gactcgacgt agacctcgcg
     7501 cggcgaggtg ccggcctccg tcagctcctg cgccggcagg ccgccgtagc cgtcgaccgc
     7561 gtcgtcggcc tcgggcggtg gcgcgtcggc gtcgatgccg ccggccagca gcgcgtgcgc
     7621 atgcgcgtcg gagccgaacc agagccggta cgagcggttg gcgaacagca actgcccctg
     7681 ctgcaccgac agcaccgaca ccgcggcgtc caggccctcc agcacggtcg tgaagcgctc
     7741 gtgcgaggcc gagagctggt cgcgcacccg cttggcctcg gtgatgttgg tgaccgaggt
     7801 catccagccg gtctgcacgc ccttggcgtc gatcagcggc gagacgtaca tgcgcgcgtc
     7861 gaacaccgag ccgtccttgc gcatgatctt gacctcgatg ccgccggccg ggctgcggcc
     7921 ctggagctcc tgctgcagca ggcgcgagtt ctcctcgatg cggtcgtgcg gccagtgcgg
     7981 gaagggcggc aggcggccga cgagctcgtt gtccgagaag cccgtcatcg cgcagaacgc
     8041 cgggttcacg taggtgatgc ggccctccat gtccatcgcg cgcatgccgg tgagcatgga
     8101 gttctccatc gcgcgccgga agttcgtctc gtgcaccagc gcgctctgga tctgggcccg
     8161 ccgccgcacg tggcgccagg tgcccagcag catccagacg gtcaggccgg acagggcgag
     8221 caccatccag aacagcgagt tggaaatcag cccgatggac gtccggtagc cgagcccgcg
     8281 caggatgagg ccgttggagg cgggcgcgag cggcgcctcg aacgcgatcg agggcacggc
     8341 cgacggccgc ccgggcatcg aggtgatggt gctggcgagc agctcctcgt gctcgtcgag
     8401 cacctcgatc gcgcgccgct tggtgacctc ctcgggcacg gcgtggcgca gcagggcctc
     8461 gatcgagtac tccgccacca gcgtgccggc gaagttggtg tgcgagatca gcggcagctg
     8521 cacctggaac acggcggtgc cgtcgacgtc gatgaagggc gccgagtact gcggcaggtg
     8581 cgtgttgcgc gcggcgacga aggcggcctc ggacgccgtc ttcgcgccgg ccacgggcag
     8641 cgacggatcc tccgagctcc cgcgcaggct ggacgcgccg cggaagatcg agccgaggaa
     8701 cgtcgcgcgc ggcttgcggt cgggacccag ccagaccacc tgcgtgatct cgggccgggc
     8761 gggcgcgaag ctggccgtct gggtgaagaa gctgtcggtg tcgacgtcgt ggccgacgat
     8821 gtcgcgcgtc aggcggacca tcaggtcctg gtcctcgctc aggcgcgagc ggatctgctg
     8881 ctgcgccatc tccgtgtcgc ggcgcaccgc gtcggtggcg cgctcgatct cctcgctgcg
     8941 cagcgtccag aacgcggcga cgatggcggc gaggaacagc agcaccgaga ccagcgggcc
     9001 cagcgtcgcg aagcgatcct ggcgcgccgc ggactggctg cgccaccagc ggccgaacag
     9061 gcgctgggtc caggaaggcg agcggggcgg cgcgggctcg tgggcagtct gcatgccgtc
     9121 attatcgagg gcgcgcccca cggccgcccg gtgggcgagg gggcatttcc cgaccaggct
     9181 ttccctcgat aattcatgat gtgaaacgcg ctgccgtcat ttgaaatccg cagggacttg
     9241 tgcgacactc cgccggccct aggatctagg gctcccgctc taaacgccta atgccaggag
     9301 acgccgcgat gtccgccatc ccccaatcct tcccgggtgc cgccgccaac gacagcgatg
     9361 cccaggaaac ccgcgaatgg acggacgccc tgtcggccgt catcgccgcc gaagggcgcg
     9421 agcgcggcca tttcctgatc gagcagctgc tggaccacgc gcgccagcac ggcatcgacc
     9481 tgccgttctc cgccacgacc gcctacgtca acaccatcgc gcccgaggac gaggagcgct
     9541 tcccgggcaa catcgacatc gaggagcgcc tgcgcgccta catgcgctgg aacgcgatgg
     9601 cgatggtcgt gcgcgcgaac cgcctgcatc cggccgacgg cggcgacctc gggggccacc
     9661 tgtcctcgtt cgcgtccctc gcgacgatgc tgggctgcgg cttcaaccac ttctggcacg
     9721 ccgagagccc cgaccacggc ggcgacctgc tctacattca gggccactcg tcgcccggca
     9781 tctacgcccg cgcctacctg gaaggccgca tcaccgagga acagctggtg cacttccgcc
     9841 aggaggtcga gggcaagggc ctctcgagct acccgcaccc gaagctgatg ccggagttct
     9901 ggcagttccc gacggtgtcg atgggcctgg gcccgctgat ggcgatctac caggcgcgct
     9961 tcctgaagta cctgcacgcg cgcggcatcg ccgacacgag caagcgcaag gtctgggtct
    10021 tctgcggcga cggcgagatg gacgagcccg agtcgctcgg cgcgatcggc ctggccgcgc
    10081 gcgagaagct cgacaacctg atcttcgtcg tcaactgcaa cctgcagcgc ctggacggc
//
LOCUS       DASVLN010000862         1998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_99862, whole genome shotgun sequence.
ACCESSION   DASVLN010000862 DASVLN010000000
VERSION     DASVLN010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99862"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VF453_20310"
     CDS             <1..1128
                     /locus_tag="VF453_20310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7690061.1"
                     /translation="RAVALAAELQALQDDLRREGATTPLVAHAEELCARARRLLAHRH
                     HLVEALHRLTQEMSAGMGELAEDGSWVQGQAQSLQQSLGEAPSVRSVRAAGEMLARTR
                     ARQRQVRTERDKARLALRESIQQMLDELDALGDHTGRFTDSVARYVDTVASADSLESL
                     AGVVRQMVEESRAVHGAVSQAQERLREGHRRTADLEAQVRTLESELKRLSDEVSTDVL
                     TEVANRRGLMQAFEVEVARLDRQEGEGALAIGLLDIDNFKKLNDTLGHAVGDTALKAL
                     AGHVQAQLRPVDVVARFGGEEFVVLLPGTAVGEAQVTLTRLQRTLSASLFMHDGREVF
                     VTFSAGVTAFRPGEALEAALERADEALYEAKRTGKNRTCIG"
     gene            complement(1142..1558)
                     /locus_tag="VF453_20315"
     CDS             complement(1142..1558)
                     /locus_tag="VF453_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690062.1"
                     /translation="MRFPFAFPRCILAALAAALAASGCVSAEMTQRYEQSLARWKGAP
                     VADLERAWGRPQKQEPRADASVVATYVVHHDMDNGQQAQRVTSLADGSGHVGTIVTGI
                     AVAPTAPVTCTTRFVERAGIVESWTFSGVGCGAPDA"
     gene            complement(1646..>1998)
                     /locus_tag="VF453_20320"
     CDS             complement(1646..>1998)
                     /locus_tag="VF453_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690063.1"
                     /translation="CASGASMDARYDQSLARWKGEPRERLLAAWGAPNFEERRPDGTT
                     VVVYVVHHDIEHRPQGPSVVTTVDPHGRAVTLMTPETLSAPVVPVTCTTRFVLRGGVV
                     DSWTFDGLACGAPG"
BASE COUNT          280 a          720 c          757 g          241 t
ORIGIN      
        1 cgtgccgtcg ccctcgccgc cgaactgcag gcgctgcagg acgatctccg gcgcgagggg
       61 gcgacgacgc cgctggtcgc gcacgccgag gagctctgcg cccgcgcgcg ccggctgctc
      121 gcgcatcgcc accatctcgt cgaggcgctg caccggctga cgcaggagat gagcgccggc
      181 atgggcgagc tcgccgagga cggcagctgg gtgcagggcc aggctcaaag cctgcagcag
      241 agcctgggcg aggcgcccag cgtgcgttcg gtgcgcgccg cgggcgagat gctcgcgcgc
      301 acgcgcgccc gccagcgcca ggtgcgcacg gaacgcgaca aggcccggct cgccctgcgc
      361 gaatcgatcc agcagatgct cgacgagctc gacgccctcg gcgaccacac gggccgcttc
      421 accgacagcg tcgcgcgcta cgtcgacacc gtcgccagcg cggactcgct cgagagcctg
      481 gccggcgtcg tgcgccagat ggtggaggag agccgcgcgg tgcacggcgc cgtcagccag
      541 gcgcaggagc gactgcgcga gggccatcgt cgcaccgccg acctcgaggc gcaggtgcgc
      601 acgctcgagt ccgagctgaa gcggctgtcc gacgaggtct cgaccgacgt gctcaccgag
      661 gtcgccaacc ggcgcgggct gatgcaggcc ttcgaggtcg aggtcgcgcg cctggatcgc
      721 caggaaggcg agggcgcgct ggcgatcggc ctgctggaca tcgacaactt caagaagctc
      781 aacgacacgc tggggcatgc ggtcggcgac acggcgctga aggcgctcgc cggccacgtg
      841 caggcgcagc tgcggccggt ggacgtggtc gcgcgcttcg gcggcgagga gttcgtcgtg
      901 ctgctgcccg gcacggcggt cggcgaggcc caggtcacgc tgacccgcct gcaacgcacc
      961 ctcagcgcga gcctgttcat gcacgacggc cgcgaggtct tcgtgacctt ctccgccggc
     1021 gtcaccgcgt tccgcccggg cgaggcgctc gaggccgccc tcgagcgcgc cgacgaggcg
     1081 ctctacgagg ccaagcgcac cgggaagaac cggacctgca tcggctgatc gccggcccgc
     1141 gtcacgcgtc gggcgcgccg cacccgacgc cggagaaggt ccacgattcg acgatccctg
     1201 cgcgttcgac gaagcgcgtg gtgcaggtga ccggcgcggt cggcgcgacg gcgatgccgg
     1261 tcacgatcgt gccgacgtgc ccgctgccgt ccgcgaggct cgtcacccgc tgcgcctgct
     1321 ggccgttgtc catgtcatgg tgcaccacgt aggtcgccac cacgcttgcg tcggcacgcg
     1381 gctcctgctt ctgcggccgg ccccaggcgc gttccaggtc ggcgacgggc gcgcccttcc
     1441 agcgggcgag ggactgctcg tagcgctggg tcatctcggc ggagacgcag ccggaggcgg
     1501 ccagcgccgc ggcaagggcg gcgaggatgc agcgcgggaa ggcgaagggg aaacgcatgg
     1561 cggacgacgt gctgcgcgag cggcggtgat ggggctggca cgatatcacc cggcggcggg
     1621 cgcacggagc gcggtgcggc cggcgctatc cgggcgcgcc gcacgccagc ccgtcgaagg
     1681 tccacgaatc gacgacgccg ccgcgcagca cgaagcgcgt cgtgcaggtg accggcacca
     1741 ccggggccga cagcgtctcg ggcgtcatga gggtcacggc ccggccgtgc gggtcgaccg
     1801 tcgtgacgac gctcgggccc tgcggccggt gctcgatgtc gtggtgcacg acgtagacga
     1861 cgaccgtggt gccgtccggt cgccgctcct cgaagttcgg cgcgccccag gcggcgagca
     1921 ggcgctcgcg cggctcgccc ttccagcgcg cgagcgactg gtcgtagcgc gcatccatcg
     1981 acgcgcccga cgcgcatc
//
LOCUS       DASVLN010000863         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_530530, whole genome shotgun sequence.
ACCESSION   DASVLN010000863 DASVLN010000000
VERSION     DASVLN010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530530"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..429)
                     /locus_tag="VF453_20325"
     CDS             complement(106..429)
                     /locus_tag="VF453_20325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690064.1"
                     /translation="MAAAALRNRIENLIAMAQLLERVDADPTAIGAQQYRHLVRTVQE
                     LLSDAQLPDDALRAVLKASPACATLYENLHYDRSGLSQTPLDAAVASEMAAAELINAV
                     RAKAH"
     gene            complement(546..2339)
                     /locus_tag="VF453_20330"
     CDS             complement(546..2339)
                     /locus_tag="VF453_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEX7690065.1"
                     /translation="MNTPKPTTAPAPADEAVKPANFLRNIIERDLEAGTYAQRQWGGG
                     PGDAAHHAAGVPDPARVRLRFPPEPNGYLHVGHAKSICLNFGLARDYGGVCHLRFDDT
                     NPEKEEQEYVDGIKENVRWLGFDWESPQGSHLFYASDDFDFMYRAAEALVQDGLAYVD
                     EQTPEQMRANRGDFGTPGTESPFRARTPEENLRELRAMRDGAYPDGAKVLRARIDMAS
                     PNINMRDPTLYRIRRAHHHNTGDRWCIYPMYTFAHPIEDALERITHSICTLEFEDQRP
                     FYDWLLENLARLGLLAHPLPKQYEFARLNLTYVVTSKRKLRQLVEEKHVAGWDDPRMP
                     TLVGMRRRGYTPGALQLLAERTGATKHNTWIDYSTLDIALRDDLEGSAARAMAVLDPI
                     PLKLRNWDEVFGAGHREPCGAPAHPHHPEMGQRAFVLGPELWIERDDFLEVPVKGYQR
                     LFPGNKVRLKYGVIVECTGCEKDADGRVTAVLADVVPDTKSGTPGADAVKVKGTITWV
                     SAHDGVPVEFRLYDRLFKVPQPGDDLGADLNPDSMRVTRGYVEPAAGALAAETRIQFE
                     RHGYFVADRVDHRPGAAVFNRVATLKDSWAR"
BASE COUNT          321 a          892 c         1020 g          403 t
ORIGIN      
        1 cggccgggag ggcgcggagg ccgggggaga tggccgagcg gccgggcgcg tggcgccggc
       61 cgcgggcgga gggacgtccc gggaaggacg tcgcgagggg caggctcagt gcgccttggc
      121 gcgcacggcg ttgatcagct cggcggccgc catctcggag gcgacggcgg catccagcgg
      181 ggtctgcgac aggcccgagc ggtcgtagtg caggttctcg tagagcgtcg cgcaggcggg
      241 cgaggccttc agcacggcgc gcagggcgtc gtcgggcagc tgggcgtcgg acagcagctc
      301 ctgcaccgtg cgcacgagat ggcggtactg ctgcgcgccg atggcggtcg gatcggcatc
      361 cacgcgctcc agcagctggg ccatggcgat caggttctcg atgcggttgc gcagggcggc
      421 ggcagccatc gcgggggact ccttcgggtg atgcggcggg cggcggcagg ccgcccatgc
      481 ttcccgaatt cgggcccgcg ggccggaatt caagggccgc cgcggcgatt cgcgccgccg
      541 gcggactacc tggcccagct gtccttgagc gtcgccacgc gattgaacac cgcggcgccc
      601 ggccgatggt cgacgcggtc ggcgacgaag tagccgtggc gctcgaactg gatgcgcgtc
      661 tcggccgcga gggcgccggc cgcgggttcg acatagcccc gggtgacgcg catgctgtcc
      721 ggattcaggt cggcgcccag gtcgtcgccc ggctgcggca ccttgaacag gcggtcgtac
      781 aggcggaact cgaccggcac gccgtcgtgc gccgagaccc aggtgatcgt gcccttgacc
      841 ttgaccgcgt cggcgccggg cgtgccgctc ttcgtgtcgg gcacgacgtc cgcgagcacc
      901 gcggtgacgc ggccgtcggc gtccttctcg cagcccgtgc attcgacgat cacgccgtac
      961 ttcagccgca ccttgttgcc ggggaacagg cgctggtagc ccttgaccgg cacttccagg
     1021 aagtcgtcgc gctcgatcca gagctcgggc ccgagcacga acgcgcgctg gcccatctcc
     1081 ggatgatgcg gatgcgcggg cgcgccgcag ggctcgcggt ggccggcgcc gaagacctcg
     1141 tcccagttgc gcagcttcag cgggatcggg tcgagcacgg ccatcgcgcg cgcggcgctg
     1201 ccctcgaggt cgtcgcgcag ggcgatgtcc agcgtggagt agtcgatcca cgtgttgtgc
     1261 ttggtcgcgc ccgtgcgctc ggcgagcagt tgcagcgcgc ccggcgtgta gccgcgccgg
     1321 cgcatgccga cgagggtggg catgcgcggg tcgtcccagc cggcgacgtg cttctcctcg
     1381 acgagctggc gcagcttgcg cttgctcgtg acgacatagg tgaggttcag gcgcgcgaac
     1441 tcgtactgct tcggcagcgg atgggcgagc agcccgaggc gcgcgaggtt ctcgagcagc
     1501 cagtcgtaga agggccgctg gtcctcgaac tcgagcgtgc agatgctgtg cgtgatgcgc
     1561 tcgagcgcgt cctcgatcgg gtgcgcgaac gtgtacatcg ggtagatgca ccagcggtcg
     1621 cccgtgttgt ggtggtgcgc gcgccggatc cggtacagcg tcgggtcgcg catgttgatg
     1681 ttcgggctcg ccatgtcgat cctggcccgc agcaccttgg cgccgtccgg ataggcgccg
     1741 tcgcgcatgg cgcgcagctc gcgcaggttc tcctcgggcg tgcgcgcgcg gaacgggctc
     1801 tcggtgccgg gcgtgccgaa gtcgccgcgg ttggcgcgca tctgctcggg cgtctgctcg
     1861 tcgacgtagg cgaggccgtc ctgcaccagc gcctcggcgg cgcggtacat gaagtcgaag
     1921 tcgtcgctcg cgtagaacag gtgcgagccc tgcggcgact cccagtcgaa gccgagccag
     1981 cgcacgttct ccttgatgcc gtcgacgtac tcctgctcct ccttctcggg gttggtgtcg
     2041 tcgaagcgca ggtggcagac gccgccgtag tcgcgcgcga gcccgaagtt caggcagatg
     2101 ctcttggcgt ggccgacgtg caggtagccg ttgggctcgg gcgggaagcg caggcgcacc
     2161 cgcgccggat cgggcacgcc cgcggcatgg tgcgccgcgt cgccggggcc gccgccccac
     2221 tgtcgctgcg cgtacgtgcc ggcctcgagg tcgcgctcga tgatgttgcg caggaagttg
     2281 gccggcttga cggcctcgtc ggccggcgcg ggggcggtgg tgggcttggg ggtgttcatg
     2341 acgcggtcga ttctagtggc ccggggtgcg ggcacgcgcg gcagcggtcg ggcgccacgc
     2401 cgcccaccct ctcaagaggg atggtcgccg ggcccgccgg ttcctacgat cgcggctccg
     2461 ctggccgacg cgtgccgccg gacgtcccag gagcccgcca tgaccgccat gacccaccat
     2521 cccgcgaccc gctggatcga tgccgccgcc caggtgctca tcggcgcgac ggccgtggtg
     2581 gcggcctgca tcgcgctcac cggcaccggg ctgcgcgcgc gcggcgaggc gccggc
//
LOCUS       DASVLN010000864         8275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_315112, whole genome shotgun sequence.
ACCESSION   DASVLN010000864 DASVLN010000000
VERSION     DASVLN010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8275
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315112"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..792)
                     /locus_tag="VF453_20335"
     CDS             complement(145..792)
                     /locus_tag="VF453_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690066.1"
                     /translation="MNGQRLKTALAMTAAALALAACSRTDGTTPSRENFEAGLRSFLA
                     DGHDQLCLGMYDWPIDLTEAEAGAHGRHAVQLPVFEKLGLARSEIVAVPRSADNPDGA
                     VKRYSLTDEGRKYYRPHPHKGRDGVAHKDDFCVARISLAQVEGWQLDPADAQHAAATV
                     SYTYHIEPAPWMKDPEAQRVLPMVAEVIRGAEGGLKMHQGFVRDQHGWVPVPGAV"
     gene            complement(887..1498)
                     /locus_tag="VF453_20340"
     CDS             complement(887..1498)
                     /locus_tag="VF453_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690067.1"
                     /translation="MIRVRVALAVAMLALLAGCHGREARLPTRANFTAAVADYLAQRG
                     RLCLAKYDWPIVVPDADARADDADARQMRLLESLGLVAATDAPAGRRYALTAAGQKYY
                     VRVPVVVATATQRVTHPADFCVGRLSLDRVFGWEPPQRLGGRTVSSVLFSYRVDPAPW
                     MRTPDARRAFPLAVRAIDNAGTLQLRLGVHLTRDGWVADELSS"
     gene            complement(1513..2955)
                     /locus_tag="VF453_20345"
     CDS             complement(1513..2955)
                     /locus_tag="VF453_20345"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX7690068.1"
                     /translation="MRKLIFGTAAALLALAVTACGGGGDDPSDGGTGTGTGTGTGTST
                     GTTTALSVAAQVGQKAFFDQTLSGGRNMSCATCHSPQYAYGPPNSLSVQLGSDATKAG
                     FRAVPSLRYKDATPPYSDSAANPDGVTLAAAGGGFMWDGRAATLADQAALPLLNPVEM
                     NAPSKAAVVSAVQAGPYADLFRKAFGANVFDDVDTAFADLGAAIQAFEHEDISFHPYT
                     SKFDLYVHNKIGGTLTAAETRGLRIFNGGDTGNCAACHYAGVNFDGNQGLMTDFTYQA
                     IGAPRNDLSIPGNPDPVPADADASFFDMGLCGPFRTDHAPAPAGSADTWCGKFKVPTL
                     RNAATRGVFFHNGVFHSLNQVVHFYDTRDTHPEYWYPSTGGSGAPVATPSYALQPTVV
                     PGATVMKFNDLPAAQQGNVDEEVPLGTGITPPGNDGATTLGDGVQPRAPGSAPSMSEQ
                     DMADLLCFLSTLSDGYQPPATAPTTGACVE"
     gene            complement(3123..5486)
                     /locus_tag="VF453_20350"
     CDS             complement(3123..5486)
                     /locus_tag="VF453_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7690069.1"
                     /translation="MKRNTAQRRAACVLTPVTLACALAACGGDGGGSTPVNDTTYTGK
                     VTASAFKADASGDPKLTAGYYAGATVFLDANGNGVKDANEPSTTTDATGKFVLAVPAG
                     TSGQIVADIPTTATNTASGAAVPAHLILRASAAQVTDQGAGSIVISPLSSEVQRLVEA
                     NGSTYAAEKANVAARLTGPAFNKGTATVSAADALADVNTLTGAEQYAVLYESNSLANR
                     YTYATAKLDRHDKYPDALAVAGGDPRLGSSDTRAPITFAQSQQAAFNIEGVPAYDNIF
                     VIMEENKSTDAILGNTTYAPYVNQLLSKYNQLTTYYATGNPSEPNYTALGGADDFGIT
                     DDSWWGCGAQGANAIKDVAFAGGTASDGQPLPAAGAMPIMDSAHLAGFSTTAGAACSA
                     SPTTGTNHNLPGDNLFTLIAKAGLTIRSYSESMNPGQDVRTDSVAQNVSEAYTGTDVD
                     GTTVTDTKPYPMPGGLYKVKHGPSIDFQAARSLPDFFANNRTIFGTQYTANDWKTTSP
                     YVTANGGSYDPTNWNYDQFSTDLAAGDVGNINFIMPDQCDDMHGVGTDTDSCNGGANG
                     NNGQNNSVKRADIYLNKVITAIQNSALWKNPQKRVAIVVMFDEGEGSSTSCCGWNAGG
                     KNSGAAPVTVSASGVATAASQPTGYANGNIGHGNSIFAVITNRQDVGTAPKGIKDSDA
                     YSHFSFVRTLQDMFQLADPAVDASYLNRAKYTEAFVAANITALPEFAGSADTHFDSVR
                     PINHAYVIPAGYVQKLNPADIGAIASGPAVSVQTGPDANQTNVWTLK"
     gene            complement(5705..6016)
                     /locus_tag="VF453_20355"
     CDS             complement(5705..6016)
                     /locus_tag="VF453_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690070.1"
                     /translation="MPAPMHYLSFDLSDDDHGVVTIEALASTSLAQHDAVMAEVRQVL
                     DWAWRAFPLAHGPIDDGGDWDDDLQVNAEPGGWHAVTLTISASPAFAREFAATFGGRD
                     E"
     gene            complement(6089..>8275)
                     /locus_tag="VF453_20360"
     CDS             complement(6089..>8275)
                     /locus_tag="VF453_20360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX7690071.1"
                     /translation="RYAAPVQPRRRGAAPATESYRGRSGHVHVPAALEGIVLGVFGLD
                     NRTVLKRNSADPANTHTLSIPTLKQLYNFPANSAAGQTIGIVSLSGYAIHDIQLMFNG
                     LGAGYAMPTVHDVAVHGTNTGNDPYGETTQDIGIAAAAAKGSAIAVYITQGTQAGWID
                     LVHRVAHPNAGDPSCSVLSSSWYICNGDDAATLAAEGVTTSFVNAVTAAFQDAAIQGI
                     TVCIACGDTGSNSKVNDGRAHVQYPGSDPWVVSVGGTTVGNVSGSSFEEYVWNDPSPS
                     DPSHWGTTGGGVSDFFGLPSYQGAAGVPASVNPGHRVGRGVPDVAANASYNAGYSGIF
                     HNGSPMIGNGTSASAPLWAGFVATLNAAMGTRLGFINPILYSKGSIAFRDIAPGGGPA
                     NNANGGVAGYPAGDGWDACTGWGSIDGGKMLNMLRGAGLPPGLAVFNNRLYMAWKGIE
                     HDQRIWFSNFDGAGWAPQQFVGGVATSCGVSLAVFNNKLHMAWKGMDADERIWCSSFD
                     GATWAPQRFVPGVATSTGPAIAVYNGRLYAAWKGMVSDQRIWWSSFDGTSWAPQQFVP
                     GVATSVGPQLAVFQGSLYAIWKGMNNDQGLWYSHFNGAAWAPQRQVPGVGTSEGASIA
                     VFNNRLYAAWKGMNDDQRIWYASFDGTSWTAQAMVAGVGTSVGPQLQVYGGLLHMTWK
                     GINGDDRVWHSTFNGTSWAPQQQIVGIGTNPFDLRQPEAHAEPAQA"
BASE COUNT         1049 a         2734 c         3127 g         1365 t
ORIGIN      
        1 cgacgatggc ctcggccgcg tgcggctcca tgccctgctg gcgcatcgtc gcgacgagcg
       61 cctgcggcgc ctggccgcgc tcgaggttgt gcaccagcca gtgccccagc tgcggcgcga
      121 agcggagaag gcgcgccatg cgcgtcagac cgcgcccggc accggcaccc agccgtgctg
      181 gtcgcgcacg aagccctggt gcatcttcag gccgccctcg gcgccgcgga tgacctcggc
      241 gaccatcggc agcacgcgct gcgcctcggg atccttcatc cacggcgccg gctcgatgtg
      301 gtacgtgtag ctgacggtcg cggccgcgtg ctgcgcgtcg gccgggtcga gctgccagcc
      361 ctcgacctgg gcgaggctga tgcgggcgac gcagaagtcg tccttgtggg cgacgccgtc
      421 gcggcccttg tgcggatgcg ggcgatagta tttgcggccc tcgtcggtca gcgaatagcg
      481 cttgaccgcg ccgtccgggt tgtcggcgct gcggggcacc gcgacgatct cgctgcgcgc
      541 gaggcccagc ttctcgaaca ccggcaactg caccgcgtgg cggccgtggg cgccggcctc
      601 ggcctcggtc aggtcgatgg gccagtcgta catgcccagg cacagctggt cgtggccgtc
      661 ggcgaggaag ctgcgcaggc cggcctcgaa gttctcgcgc gagggcgtcg tgccgtcggt
      721 acggctgcag gcggccagcg cgagggcggc cgcggtcatc gccagggcgg tcttgagtcg
      781 ttgtccgttc atgtcgtggt cttcaggctt cggggttcgg ggttcggggt tcgaggattg
      841 cgggatctgg cgaggacggg tgcgggatcg tctcgcgcga gggacatcag gacgagagct
      901 cgtcggcgac ccagccgtcg cgcgtgaggt gcacgcccag gcgcaactgc agcgtgcccg
      961 cgttgtcgat cgcgcgcacg gcgagcggga aggcgcggcg ggcgtcgggc gttcgcatcc
     1021 acggcgccgg gtccacccgg tacgagaaca gcaccgagct cacggtgcgg ccgccgagcc
     1081 gctgcggcgg ctcccagccg aacacgcgat cgagcgacag ccggccgacg cagaagtcgg
     1141 cgggatgcgt gacgcgctgc gtggcggtgg cgaccaccac cggcacgcgc acgtagtact
     1201 tctgccctgc ggcggtcagc gcgtagcggc gtccagcggg cgcgtcggtg gcggcgacga
     1261 ggccgagcga ctcgagcagg cgcatctgcc gcgcgtcggc gtcgtcggcg cgggcgtcgg
     1321 cgtccggcac gacgatcggc cagtcgtact tggccaggca caggcggccg cgctgggcca
     1381 ggtagtcggc gaccgcggcg gtgaagttgg cgcgcgtcgg cagccgcgcc tcgcggccgt
     1441 ggcagccggc cagcagcgcg agcatggcga cggcgagcgc gacgcggacc cggatcatgg
     1501 ccggccccac ggtcactcga cgcaggcgcc ggtggtcggc gcggtggcgg gcggctggta
     1561 gccgtcgctc agcgtggaca ggaagcacag gaggtcggcc atgtcctgct ccgacatcga
     1621 cggcgccgag cccggcgcgc gcggctgcac gccgtcgccg agcgtggtgg cgccgtcgtt
     1681 gcccggcggc gtgatgccgg tgcccagcgg cacctcctcg tcgacgttgc cttgttgcgc
     1741 ggcgggcagg tcgttgaact tcatgacggt cgcgccgggc acgacggtcg gctgcagcgc
     1801 gtacgacggc gtggcgaccg gggcgccgga gccgccggtc gaggggtacc agtactcggg
     1861 atgggtgtcg cgggtgtcgt agaagtgcac gacctggttc agggagtgga agacgccgtt
     1921 gtggaagaag acgccgcgcg tggccgcgtt gcgcagcgtc ggcaccttga acttgccgca
     1981 ccaggtgtcg gcggagcccg cgggcgccgg ggcgtggtcg gtgcggaagg ggccgcacag
     2041 gcccatgtcg aagaagctcg cgtccgcgtc ggccgggacc ggatccgggt tgccggggat
     2101 cgagaggtcg ttgcgcggcg caccgatggc ctggtacgtg aagtccgtca tcaggccctg
     2161 gttgccgtcg aagttcacgc ccgcgtagtg gcaggccgcg cagttgccgg tgtcgccgcc
     2221 gttgaatatt cgcaggcccc gggtctccgc cgccgtcagg gtgccgccga tcttgttgtg
     2281 gacgtagagg tcgaacttgc tcgtgtacgg atggaagctg atgtcctcgt gctcgaaggc
     2341 ctggatcgcc gcgccgaggt cggcgaacgc ggtgtcgaca tcgtcgaaca cgttcgcccc
     2401 gaacgccttc ctgaacaggt cggcgtaggg gccggcctgc accgcggaga cgacggcggc
     2461 cttggacggg gcgttcatct cgaccgggtt gagcagcggc agcgcggcct ggtcggcgag
     2521 cgtcgccgcg cggccgtccc acatgaagcc gccgccggcg gcggccagcg tgacgccgtc
     2581 ggggttggcg gcggagtcgg agtagggcgg cgtcgcgtcc ttgtagcgca gcgacggcac
     2641 ggcgcggaag ccggccttcg tcgcgtccga gccgagctgc accgacaggc tgttgggcgg
     2701 cccgtacgcg tattgcggcg agtggcaggt ggcgcacgac atgttcctgc cgcccgacag
     2761 cgtctggtcg aagaacgcct tctgcccgac ctgggccgcg acgctcagcg cggtcgtggt
     2821 gcccgtgctc gtacctgtac cggtaccggt accggtgccc gtgccgccgt cggacgggtc
     2881 gtcgccgcca cccccgcatg ccgtcaccgc cagggcgagc agcgccgccg cggttccgaa
     2941 gatcaacttg cgcatcctgc ttctctgctc tcgttcatcg ccgcattcgc atgtcgtcgg
     3001 ggacggcatg cggatgggac gatgccccaa tggaaaggac gggccgccct ggagccggcc
     3061 cgtcgctcgg atcacgaagc ccgcgggcgg ggcggcgagg cgccccgtcc gcgcagcgtc
     3121 ccttacttca gcgtccagac gttggtctgg ttggcgtccg ggccggtctg caccgacacg
     3181 gccgggccgc tggcgatcgc gccgatgtcg gccgggttca gcttctggac gtagccggcc
     3241 gggatgacgt aggcgtggtt gatcggacgc accgagtcga agtgcgtgtc cgcgctgccc
     3301 gcgaattccg gcagggccgt gatgttcgcg gcgacgaacg cctcggtgta cttcgcgcgg
     3361 ttcaggtacg acgcgtcgac ggcggggtcg gcgagctgga acatgtcctg cagcgtgcgc
     3421 acgaacgaga agtggctgta ggcgtcgctg tccttgatgc ccttcggcgc ggtcccgacg
     3481 tcctggcgat tggtgatcac cgcgaagatc gagttgccgt ggccgatgtt gccgttggcg
     3541 tagcccgtcg gctgcgacgc cgccgtggcc acgccgctgg ccgacaccgt caccggcgcc
     3601 gcgcccgagt tcttgccgcc ggcgttccag ccgcagcagg aggtgctcga gccttcgcct
     3661 tcgtcgaaca tgacgacgat ggcgacgcgc ttctgcgggt tcttccacag cgccgagttc
     3721 tggatcgcgg tgatcacctt gttcaggtag atgtcggcac gcttgacgct gttgttctgg
     3781 ccgttgttgc cgttcgcgcc gccgttgcag gagtcggtgt cggtgccgac gccgtgcatg
     3841 tcgtcgcact ggtcgggcat gatgaagttg atgttgccga catcgccggc ggccaggtcc
     3901 gtgctgaact ggtcgtagtt ccagttcgtc gggtcgtagc tgccgccgtt ggcggtcacg
     3961 tagggcgacg tcgtcttcca gtcgttggcc gtgtactgcg tgccgaagat cgtgcggttg
     4021 ttggcgaaga agtccggcag gctgcgggcc gcctggaagt cgatcgacgg gccgtgcttg
     4081 accttgtaca ggccgcccgg catcgggtag ggcttcgtgt cggtgaccgt cgtgccgtcg
     4141 acgtcggtgc cggtgtacgc ctcggagacg ttctgggcga cgctgtcggt gcggacgtcc
     4201 tggcccgggt tcatcgactc gctgtagctg cggatcgtca ggccggcctt ggcgatcagc
     4261 gtgaacaggt tgtcgcccgg caggttgtgg ttggtgccgg tggtcggcga cgcgctgcac
     4321 gcggcgccgg cggtggtgct gaagcccgcc aggtgcgcgc tgtccatgat gggcatggcg
     4381 ccggcggccg gcagcggctg gccgtcggag gcggtgccgc ccgcgaaggc gacgtccttg
     4441 atcgcgttgg cgccctgcgc gccgcagccc caccagctgt cgtcggtgat gccgaagtcg
     4501 tcggcgccgc ccagcgcggt gtagttcggc tcggacgggt tgccggtcgc gtagtacgtc
     4561 gtcagctggt tgtacttgga caggagctgg ttgacgtagg gcgcgtaggt cgtgttgccc
     4621 aggatcgcgt ccgtcgactt gttctcctcc atgatgacga agatgttgtc gtacgccggc
     4681 acgccttcga tgttgaaggc cgcctgctgg gactgcgcga aggtgatggg cgcgcgggtg
     4741 tcgctcgagc ccaggcgcgg gtcgccgccg gccacggcga gcgcgtcggg atacttgtcg
     4801 tggcgatcga gcttggcggt cgcgtaggtg tagcggttgg ccagcgagtt cgactcgtac
     4861 agcacggcgt actgctcggc gccggtcagg gtgttgacgt cggccagggc atcggcggcg
     4921 ctgacggtgg cggtgccctt gttgaaggcg gggccggtca ggcgggcggc gacgttggcc
     4981 ttctccgcgg cgtaggtgct gccgttggcc tcgacgaggc gctgcacttc gctggacagc
     5041 gggctgatga cgatgctgcc ggcgccctgg tcggtgacct gggccgccga ggcgcgcagg
     5101 atcaggtggg ccgggaccgc ggcgccgctg gcggtgttgg tcgcggtggt cgggatgtcg
     5161 gcgacgatct ggccggaggt gcccgcgggc acggccagga cgaacttgcc ggtggcgtcg
     5221 gtcgtggtcg agggctcgtt cgcgtccttg acgccgttgc cgttggcgtc gaggaagacg
     5281 gtcgcgccgg cgtagtagcc cgcggtcagc ttcggatcgc cggaggcgtc cgccttgaag
     5341 gcgctcgccg tgaccttgcc cgtgtaggtc gtgtcgttca cgggcgtcga accccccccg
     5401 tcgccgccgc aggcagcgag cgcgcacgcg agggtgacgg gcgtcaggac gcacgcggca
     5461 cggcgctgcg cagtattgcg tttcatgtcg atctcctgtg ttggtgtggc ggggccgcag
     5521 ttcccgacca gacgcgcagc ctagggatcc tttatgaaac gaaagtgtca cctcatgttt
     5581 gttgggccaa gcgcgagcgt ggcgaagggc tttcacgaaa ccgtcatcga agcggccgtt
     5641 cgattccggt gatgtggccg gtgacttccg gcacgcgttt cacgccgcgt cgagcggccg
     5701 ccgcctactc gtcgcgaccg ccgaaggtcg cggcgaactc gcgcgcgaat gccggcgacg
     5761 cactgatcgt cagcgtcacc gcgtgccagc cgccgggctc ggcgttcacc tggaggtcgt
     5821 cgtcccagtc gccgccgtcg tcgatgggcc cgtgcgcgag cggaaatgcg cgccaggccc
     5881 agtccagcac ctgccgcacc tcggccatca cggcgtcgtg ctgcgcgagg gaggtcgagg
     5941 cgagcgcctc gatcgtgacg acgccgtggt cgtcgtcgct gaggtcgaag gacaggtagt
     6001 gcatcggggc aggcatcgcg ggccaccgag catagcggct cggcgcccgc gatgcggcca
     6061 tgcccttggc gggcgcgtcc gcggttgctc aggcctgcgc gggctcggcg tgcgcctcgg
     6121 gctggcgcag gtcgaacggg ttggtgccga tgcccacgat ctgctgctgc ggcgcccagc
     6181 tcgtgccgtt gaaggtcgag tgccagacac ggtcgtcgcc gttgatgccc ttccacgtca
     6241 tgtgcagcag cccgccgtag acctggagct gcggcccgac gctggtgccc acgcccgcga
     6301 ccatcgcctg cgccgtccac gaggtgccgt cgaagctcgc gtaccagatg cgctggtcgt
     6361 cgttcatgcc cttccacgcg gcgtagagcc ggttgttgaa caccgcgatc gatgcgccct
     6421 cgctcgtgcc cacgcccggc acctgccgct gcggcgccca ggcggcgccg ttgaagtgcg
     6481 agtaccacaa gccctggtcg ttgttcatgc ccttccagat cgcgtagagg ctgccctgga
     6541 agacggccag ctgcggcccc acgctcgtgg cgacgccggg gacaaactgc tgcggcgccc
     6601 agctcgtgcc gtcgaagctg gaccaccaga tccgctggtc gctcaccatg cccttccacg
     6661 ccgcgtagag ccggccgttg tagaccgcga tcgccgggcc ggtgctcgtc gcgacgccgg
     6721 gcacgaaccg ctgcggcgcc cacgtcgcgc cgtcgaagct ggaacaccag atccgctcgt
     6781 cggcgtccat gcccttccac gccatgtgga gcttgttgtt gaagaccgcc agcgagacgc
     6841 cgcagctcgt ggcgacgccg ccgacgaact gctgcggcgc ccagcccgcg ccgtcgaagt
     6901 tcgagaacca tatgcgctgg tcgtgctcga tacccttcca cgccatgtac agccggttgt
     6961 tgaacacggc caggcccggc ggcaggccgg cgccgcgcag catgttgagc atcttcccgc
     7021 cgtcgatgct gccccagccg gtgcaggcgt cccagccgtc gcccgcggga tagccggcga
     7081 cgccgccgtt ggcgttgttg gcggggccgc cgccgggggc gatgtcgcgg aacgcgatcg
     7141 agcccttcga gtacaggatc gggttgatga agcccaggcg cgtgcccatc gccgcgttga
     7201 gcgtcgcgac gaagccggcc cacagcggcg cggaggcgct ggtgccgttg ccgatcatcg
     7261 ggctgccgtt gtggaagatg ccggagtagc ccgcgttgta gctcgcgttc gcggccacgt
     7321 cgggcacgcc gcggccgacg cggtggcccg ggttgaccga cgcgggcacg cctgcagcgc
     7381 cctggtacga gggcaggccg aagaagtcgc tcacgccgcc gccggtcgtg ccccagtgcg
     7441 agggatcgga cgggctcgga tcgttccaca cgtattcctc gaagctcgag cccgagacgt
     7501 tgccgaccgt cgtgccgccc accgacacca cccacgggtc gcttcccggg tactgcacgt
     7561 gcgcgcggcc gtcgttgacc ttggagttcg agccggtgtc gccgcaggcg atgcacacgg
     7621 tgatgccctg gatcgccgcg tcctggaacg cggccgtcac ggcgttgacg aacgaggtcg
     7681 tgacgccctc ggccgcgagg gtcgcggcgt cgtcgccgtt gcagatgtac cagctgctcg
     7741 agagcaccga gcacgaggga tcgcccgcgt tcgggtgcgc gacgcggtgg accaggtcga
     7801 tccagccggc ctgcgtgccc tgggtgatgt agaccgcgat cgccgacccc ttggccgcgg
     7861 cggccgcgat gccgatgtcc tgcgtggtct cgccgtaggg atcgttgccg gtgttggtgc
     7921 cgtgcacggc cacgtcgtgc accgtgggca tcgcgtagcc cgcgcccagg ccgttgaaca
     7981 tcagctggat gtcgtggatc gcgtagcccg agagcgagac gatgccgatc gtctgccccg
     8041 ccgcggagtt ggcggggaag ttgtagagct gcttgagcgt cgggatcgac agcgtgtggg
     8101 tgttggccgg atcggcggag ttgcgcttga gcaccgtgcg gttgtcgagc ccgaacacgc
     8161 ccagcacgat gccttcgagc gccgccggca cgtggacgtg cccgctgcgg ccgcggtagc
     8221 tctcggtggc cggcgccgcg ccgcggcggc gcggctgcac cggcgcggcg tagcg
//
LOCUS       DASVLN010000865         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_43675, whole genome shotgun sequence.
ACCESSION   DASVLN010000865 DASVLN010000000
VERSION     DASVLN010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43675"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /gene="malF"
                     /locus_tag="VF453_20365"
     CDS             <1..1051
                     /gene="malF"
                     /locus_tag="VF453_20365"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HEX7690072.1"
                     /translation="APLPRDAAVTPLTVRQTLRHRDALMAATLALPDGRVLSYGGVHE
                     FGPSAHVWDANADGSLTRIEDGKVYAPDTRTGWYVAADGDRLQPGFRVGVGFANYARM
                     LSDQEFRGPFLSIFVWTVVFAALTVLFSLSVGCTLAVLLNWEALRFRTLYRTLLFLPY
                     AVPGFISILVFKGLFNQNFGEINAILDAVFGVKPAWFADPMLARAMLVVVNTWLGYPY
                     FLVLCTGLLKAIPPDLYEASAIAGAKPLTNFFRITAPLIVKPLSPLLISAFAFNFNNF
                     VLISLLTNGRPDFLNTKIPAGTTDILVSYTYRIAFLDSGQNFGLAAAISTVIFFLVAL
                     LSIANLRVAARNARR"
     gene            1063..1953
                     /gene="malG"
                     /locus_tag="VF453_20370"
     CDS             1063..1953
                     /gene="malG"
                     /locus_tag="VF453_20370"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HEX7690073.1"
                     /translation="MAIVTGKNQRWRVVAAHAFLVALVVVTLFPLAAVVSISLRPGNF
                     ANGSLIPREISFEHWKLALGISYTTPDGVVVHPPFPVLLWLWNSVKVATIASALIVAI
                     STTAAYAFARLKFAFKSPILNGMLLLQMFPPVLALVAIYAIFDTLGRYVPALGLETHG
                     GLIVAYLGGVATHVWTIRGYFETIPTEIEECAKVDGASHFQAFVRVLLPMAVPILMVV
                     FVLAFVGTIIEYPVASVILREEANLTLAVGSKYYLADQRYLWGDFAAAAVLSGLPITI
                     VFLLAQRWIVSGLTAGGVKE"
     gene            2070..3617
                     /locus_tag="VF453_20375"
     CDS             2070..3617
                     /locus_tag="VF453_20375"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7690074.1"
                     /translation="MRSDYANSHDRALAPVMQAREADWRNGAVAYQVLVDRFAPPANL
                     EAKRALYPPPKQLHPWTDLPVHGHYLPGQKLWSHEIDFWGGDLASLRGKLDYVQGLGA
                     DVLYLNPIALAYTNHKYDTLDYRAVSPEYGTRDDVRALARDVHARGMKLVLDGVFNHM
                     GRNSALFRQAEADPHGPYRDWFVFGPQYAGGARSWALAENLPELNLENPAVRADLFTG
                     KDSVVRGWLRDGVDGWRLDVAFDIGFRYLAELTQAAHEEKPGSLVVGEIANYPKEWFP
                     SVDGVLHFTLRQVILGLAGGRMDPATATAAIERLVADADYEHLLKSWVYLDNHDTPRL
                     ATVLPDVRARRLAQVLQFTLPGSPNLYYGTELDMTGGDDPEMRAPMRWDRVTAGNAAL
                     AWNRQLVALHQTHRALRIGDFRTVTSTKLLAYERYAERAADAVVVLANPSDQPVAETV
                     MIPDSKIMDGTRMVNLLADPADPSTPAELRVHAALMDVRLPAHGVVVLQPDTAPPGGY
                     TNYKRVE"
BASE COUNT          513 a         1405 c         1202 g          606 t
ORIGIN      
        1 ggcgccgctg ccgcgcgacg cggcggtcac cccgctgacg gtcaggcaga cgctgcgcca
       61 ccgcgacgcg ctgatggccg cgacgctggc gctgcccgac ggccgcgtcc tgagctacgg
      121 cggcgtgcac gagttcggcc cgtcggccca cgtgtgggac gcgaacgcgg acggctcgct
      181 cacgcgcatc gaggacggca aggtctatgc gcccgatacg cgcaccggct ggtacgtcgc
      241 ggccgacggc gaccgcctgc agcccggctt ccgcgtcggc gtcggcttcg cgaactacgc
      301 gcgcatgctg tccgaccagg agttccgcgg tccgttcctc tcgatcttcg tctggaccgt
      361 cgtcttcgcc gcgctgaccg tgctgttctc gctgtctgtc ggctgcacgc tggcggtgct
      421 gttgaactgg gaggcgctgc gcttcaggac gctgtaccgc acgctgctgt tcctgcccta
      481 cgcggtcccc ggcttcatct cgatcctcgt cttcaagggc ctgttcaacc agaacttcgg
      541 cgagatcaat gccatcctgg acgcggtctt cggcgtcaag ccggcgtggt tcgccgatcc
      601 catgctggca cgcgcgatgc tggtcgtcgt caatacgtgg ttggggtacc cgtacttcct
      661 cgtgctgtgc accgggctgc tgaaggcgat cccgcccgac ctctacgagg cctcggccat
      721 cgccggcgcg aagccgctca ccaatttctt ccgcatcacg gcgccgctca tcgtcaagcc
      781 gctgtcgccg ctgctgatat cggcgttcgc gttcaacttc aacaacttcg tcctgatctc
      841 gctgctgacc aacggccggc ccgacttcct caacacgaag atcccagcgg gcacgacgga
      901 catcctcgtg tcctacacct accgcatcgc cttcctcgac tcgggccaga acttcggcct
      961 cgcggcggcg atctccaccg tgatcttctt cctggtcgcg ctgctgtcga tcgccaacct
     1021 gcgggtggcg gcacgcaacg cccgtcgctg aggagccgtc gcatggccat cgtcaccggc
     1081 aagaaccagc gctggcgcgt cgtcgccgcg cacgccttcc tcgtcgcgct cgtcgtcgtc
     1141 acgctgttcc cgcttgccgc ggtcgtgtcg atctcgctgc ggcccggcaa cttcgccaac
     1201 ggctcgctga tcccgcgcga gatcagcttc gagcactgga agctcgcgct cggcatctcg
     1261 tacaccacgc ccgacggcgt cgtcgtgcac ccgcccttcc cggtgctgct gtggctgtgg
     1321 aactcggtca aggtcgcgac catcgcgtcg gcgctgatcg tcgcgatctc gaccaccgcc
     1381 gcctacgcgt tcgcgcggct gaagttcgcg ttcaagtcgc ccatcctcaa cggcatgctg
     1441 ctgctgcaga tgttcccgcc ggtgctcgcg ctggtggcga tctacgccat cttcgacacc
     1501 ctcggccgct acgtgcccgc gctgggcctg gagacgcatg gcggcctgat cgtggcctac
     1561 ctgggcggcg tggcgacgca cgtgtggacg atccgcggct acttcgagac catccccacc
     1621 gagatcgagg aatgcgccaa ggtcgacggc gccagccact tccaggcgtt cgtgcgcgtg
     1681 ctgctgccga tggccgtgcc gatcctgatg gtggtgttcg tgctggcctt cgtcggcacg
     1741 atcatcgagt accccgtcgc ctccgtcatc ctgcgcgagg aggccaacct gacgctggcc
     1801 gtcggctcca agtactacct cgccgaccag cgctacctgt ggggcgactt cgcggccgcg
     1861 gcggtgctct cgggcctgcc gatcacgatc gtcttcctgc tcgcgcaacg ctggatcgtc
     1921 tcgggcctga ccgccggcgg cgtcaaggaa tgaggctcgc gattcgcccg tcgcgtcgcc
     1981 ctgcgctcgc gctggccgcc gcgctcttcg tggcggtacc cgccgccgcc atcgcacaaa
     2041 cctccgtcgc cacgacgccc ccgcccgcca tgcgctccga ctacgcgaac agccacgacc
     2101 gcgccctcgc gcccgtcatg caggcccgcg aggccgactg gcgcaacggc gcggtcgcct
     2161 accaggtgct cgtcgaccgc ttcgcgccgc ccgcgaacct cgaggccaag cgcgcgctct
     2221 acccgccgcc caagcagctg cacccgtgga ccgacctgcc ggtgcacggc cactatctgc
     2281 ccggccagaa gctgtggagc cacgagatcg acttctgggg cggcgacctc gcgagcctgc
     2341 gcggcaagct cgactacgtg cagggcctgg gcgcggacgt cctgtacctc aatccgatcg
     2401 ccctcgccta cacgaaccac aaatacgaca cgctggacta ccgcgccgtc tcgcccgagt
     2461 acggcacgcg cgacgacgtg cgcgcgctgg cgcgggacgt gcacgcgcgc ggcatgaagc
     2521 tggtgctgga cggcgtgttc aaccacatgg gccgcaactc ggcgctgttc aggcaggccg
     2581 aggccgatcc ccacggcccg tatcgcgact ggttcgtctt cggcccgcag tacgccggcg
     2641 gggcgcgcag ctgggcgctg gccgagaacc tgcccgagct gaacctcgag aaccccgccg
     2701 tgcgcgccga cctgttcacc ggcaaggact ccgtggtgcg cgggtggctg cgcgacggcg
     2761 tcgacggctg gcggctggac gtcgccttcg acatcggctt tcgctacctc gccgagctca
     2821 cccaggccgc tcacgaggag aagcccggct cgctggtcgt gggcgagatc gcgaactacc
     2881 cgaaggaatg gttcccgtcg gtcgacggcg tgctccactt cacgctgcgc caggtcattc
     2941 tcggcctcgc gggcggccgc atggatcccg cgaccgcgac cgccgcgatc gagcgcctcg
     3001 tggccgacgc ggattacgaa cacctgctga agtcctgggt ctacctcgac aaccacgaca
     3061 ccccgcgcct ggccaccgtc ctgcccgacg tgcgcgcgcg ccggctcgcc caggtgctgc
     3121 agttcacgct gcccgggtcg cccaacctct actacggcac cgagctcgac atgaccggcg
     3181 gcgacgaccc ggagatgcgc gccccgatgc gctgggatcg cgtgaccgcc ggcaacgccg
     3241 cgctggcatg gaaccggcag ctcgtcgcgc tgcaccagac gcatcgcgcg ctgcgcatcg
     3301 gcgacttccg cacggtcacc tcgacgaagc tgctcgcgta cgagcgctac gcggagcgcg
     3361 ccgcggacgc ggtggtcgtg ctcgcgaatc cgtccgacca gccggtcgcg gagacggtga
     3421 tgattcccga ctcgaagatc atggacggca cgcgcatggt caacctgctg gccgatccgg
     3481 ccgacccctc gacgcccgcg gagctgcgcg tgcatgcggc gctgatggac gtgcgcctgc
     3541 ccgcgcacgg cgtcgtcgtg ctccagcccg acaccgcccc gcccggcggc tacaccaact
     3601 acaagcgcgt ggaatgaacc gggcgccgcg ggcgggaggc ccgggcgcac gaatcacggg
     3661 tcgcgggcgc gcaccggccg gcgcggcccg ggatcagttg gcggagcagt tgtcgacggc
     3721 cgcggc
//
LOCUS       DASVLN010000866         4943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_421417, whole genome shotgun sequence.
ACCESSION   DASVLN010000866 DASVLN010000000
VERSION     DASVLN010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421417"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF453_20380"
     CDS             <1..327
                     /locus_tag="VF453_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX7690075.1"
                     /translation="ELVVGAGNIYACEALHLARIDPRTPANRLSAPRAARLLAAVRQV
                     LRRAIEAGGSTLKDFSANGAAGHYQEQAQVYGRGGQPCNTCGNPVRRIVQGQRATYFC
                     GICQKR"
     gene            543..2534
                     /locus_tag="VF453_20385"
     CDS             543..2534
                     /locus_tag="VF453_20385"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX7690076.1"
                     /translation="MTKDQALIPGLDALASWRQSLARELAGLTQFFTSNDLLSAPSAE
                     IAESLRQRMLADKLVVAFVAEFSRGKSELINAIFFADTGRRILPATPGRTTMCPVELG
                     WDEREKPMLDLLPIETRREGASLTELRATREGWKRVPLDPADPQALADALAEVTHTRK
                     ATPEEAKELGLWSETDPEDNPPLDEQGLVDVPAWRHAVINYPHPLLARGLVVLDTPGL
                     NAIGTEPELTLGLLPGAHAALFLLGADAGVTKSDLSVWNEHLGGQNMACFVVLNKVDM
                     LADPLLTPEQNEALVRKQCEVTAQTLQMPRERVFPVSARNALHARLNHDDAALEKSRL
                     PALEAALTDELLPARQQVLTHAVIAGVETLRAQVGARLRDQRRQNSEQLLELRGLRGK
                     SGARVRHLLDRVQEDAGEFERCITRLTALRAVQTRLLRDVQNQLSSERIRAEVKQLQS
                     ALNSGWLHLGARQAFLGMCDKLRHALSESEVTTSEAREMLQGTFRQLNAEFGFSLSIA
                     LPPELAAHREELAAIERGYNRYFGLSETLRMASSAFKERFVRMLVSKLRVVFETASRD
                     IELWNQSASAQMEAQLRDRRRSFRKRRESLERIQQAAGELEERVADVEAQAARIESLI
                     TDVGLRCETLAVIARRGPLGIESEHFEIPVEVGAAAATV"
     gene            2621..3796
                     /gene="mutY"
                     /locus_tag="VF453_20390"
     CDS             2621..3796
                     /gene="mutY"
                     /locus_tag="VF453_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009972864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX7690077.1"
                     /translation="MDPSAAGARPPFIAAEAAPEWNALPFAQRVIAWQRAHGRDGLPW
                     QRTRDPYRVWLSEIMLQQTQVATVLGYYARFLERFPTVEALAAAPTEDVLALWAGLGY
                     YARARNLHRCAQVVAREHGGAFPRSAEALAALPGIGRSTAAAIASFCFGERAAILDGN
                     VKRVLARVLAFERDLAQAASEKALWAIADGLLPHAPAGEPLHAAMASYTQGLMDLGAT
                     VCTIRRPACDACPAHGTCAGRAAGEPERYPVKTRKLKRGKRAGVLLWLQERDRVWLVR
                     RPDTGVWAQLWSLPEFADAVALERAVADWPGTLEWLPPFKHVLTHFDWMLQPLRLVWH
                     GRVDPQEQRAIDALRAFNGGDGSDPGAAPTAGRWVARSELDSVGVPAPIRKLLDAGP"
     gene            complement(3831..4784)
                     /gene="rapZ"
                     /locus_tag="VF453_20395"
     CDS             complement(3831..4784)
                     /gene="rapZ"
                     /locus_tag="VF453_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX7690078.1"
                     /translation="MTPDDFNDPIGEAIPYDAPVPAPGASEGGIREVVLVTGISGSGK
                     SIALHALEDAGFFCCDNLPPELLPEFLRLQRERGIGRIAVAMDVRSAGSLPRLVPLLE
                     QLREDGVKVRPVFLDAGTDTLVRRYSESRRPHPLSKGTLPTDEHRALIEAIELERELL
                     AAIRMRSSILDTSQLRPVQLRRWIRDLVGAHASQLTLVFESFAFKRGVPLDADMVFDV
                     RILPNPYYVRELRALTGRDAPVADYLRAQPEVGEMMGQIEQFLRRWLPSFEDDQRSYL
                     TVAIGCTGGQHRSVYLCETLAERFGGAVTTLLRHRELDAVD"
BASE COUNT          701 a         1781 c         1787 g          674 t
ORIGIN      
        1 gagctggtgg tcggcgccgg caacatctac gcatgcgagg cgctgcacct cgcgcgcatc
       61 gacccgcgca cgcccgccaa ccgcctgagc gccccccgcg ccgcccgcct gttggccgcc
      121 gtgcgccagg tgctgcggcg cgcgatcgag gcgggcggct cgacgctgaa ggatttcagc
      181 gccaacggcg ccgccggcca ttaccaggaa caggcgcagg tctacggccg cggcggccag
      241 ccctgcaata cctgcggaaa cccggtgcgg cgaatcgtcc agggacagcg ggcgacctat
      301 ttctgcggca tttgccagaa aaggtgaacg cgacgatttt ccggcaagtc gtatcgatat
      361 tgccgagcat gtgtccaaag gtttcaaatc gaccgtgacg cgaccgcgtc gatacccgct
      421 ggtgcgactt actccgcatt tccgcgcctg atctctcgat gcgtccggaa ccaatcgcgc
      481 tacgatcgcg gccgggcggg cgtgcaccga ccggccattc gaacgaacaa acgagcctgg
      541 gcatgacgaa agatcaagcg ctgatacccg gtctggacgc cttggcgagc tggcggcaat
      601 cgctcgcgcg cgagctcgcg ggcctgacgc aattcttcac cagcaacgat ctgctgtcgg
      661 cgccgtcggc cgaaatcgcc gaatccctgc gccagcgcat gctggcggac aaactcgtcg
      721 tcgccttcgt ggcggaattc tcgcgcggca aatccgagct gatcaacgcg atcttcttcg
      781 ccgataccgg ccgccgcatc ctgcccgcca cccccggccg cacgacgatg tgcccggtgg
      841 agctgggttg ggacgagcgc gagaagccca tgctcgacct gctgccgatc gagactcgcc
      901 gcgagggcgc ctcgctgacc gagctgcgcg ccacccgcga gggctggaag cgcgtgccgc
      961 tcgaccccgc ggatccccag gccttggccg atgcgctcgc cgaggtgacg cacacccgca
     1021 aggcgacgcc cgaggaggcg aaggagctgg gcctgtggtc ggagaccgat cccgaggaca
     1081 acccgccgct ggacgagcag ggcctcgtcg acgtgccggc gtggcgccat gcggtcatca
     1141 actacccgca cccgctgctg gcgcgcggcc tggtcgtcct cgacacgccg ggcctgaacg
     1201 cgatcggcac cgagcccgag ctgaccctgg gcctgctgcc gggcgcgcat gccgcgctgt
     1261 tcctgctggg cgccgacgcg ggcgtcacga agtccgacct gtcggtctgg aacgagcacc
     1321 tcggcggcca gaacatggcc tgcttcgtcg tcctgaacaa ggtcgacatg ctggccgacc
     1381 cgctgctgac gccggagcag aacgaggcgc tggtgcgcaa gcagtgcgag gtcaccgcgc
     1441 agacgctgca gatgccgcgc gagcgggtgt tcccggtctc ggcccgcaac gcgctgcatg
     1501 cgcgcctgaa ccacgatgac gcggcgctcg agaaaagccg cctgcccgcg ctcgaggccg
     1561 cgctgaccga cgagctgctg ccggcacgcc agcaggtgct tacccacgcg gtgatcgcgg
     1621 gcgtcgagac cctgcgcgcg caggtcggcg cgcgcctgcg cgaccagcgg cgccagaact
     1681 ccgagcagct gctcgagctg cgcggcctgc gcggcaagag cggtgcgcgg gtgcgccacc
     1741 tgctggatcg cgtgcaggag gacgcgggcg agttcgagcg ctgcatcacc cgcctgaccg
     1801 cgctgcgcgc cgtgcagacg cggctgctgc gcgacgtcca gaaccagctc tccagcgagc
     1861 gcatccgcgc cgaggtcaag cagctgcaat cggcgctcaa ctccggctgg ctgcacctgg
     1921 gcgcgcgcca ggccttcctc ggcatgtgcg acaagctgcg ccatgccctg tccgaatccg
     1981 aggtcacgac ctccgaggcg cgcgagatgc tgcagggcac cttccgccag ctcaacgccg
     2041 agttcggctt ctcgctgtcg atcgcgctgc cgccggagct ggccgcgcac cgcgaggaac
     2101 tcgccgccat cgagcgcggc tacaaccgct atttcggcct gagcgagacg ctgcgcatgg
     2161 cgtcgtccgc gttcaaggag cgcttcgtgc gcatgctcgt gtccaagctg cgcgtcgtct
     2221 tcgagacggc gtcgcgcgac atcgagctgt ggaaccagtc cgccagcgcg cagatggagg
     2281 cgcagctgcg cgaccgccgg cgcagcttcc gcaagcgccg cgagtcgctg gagcgcatcc
     2341 agcaggccgc cggcgagctg gaggagcgcg tcgccgacgt cgaggcccag gccgcgcgca
     2401 tcgaatcgct gatcaccgac gtcggcctgc gctgcgagac gctggccgtc atcgcccgcc
     2461 gcgggccgct gggcatcgag tccgagcact tcgagatccc ggtcgaggtg ggcgcggcgg
     2521 cggccacggt gtgagccggc gttccggcgc aggcgcggcg acgcaggccg cgctgcccgg
     2581 cttcgacgcg ccgcccgcgc gggctcgggc atcatcgggc atggatcctt ccgccgcggg
     2641 cgcgcgcccg cccttcatcg ccgccgaggc ggccccggaa tggaacgcgc tgcccttcgc
     2701 gcagcgcgtc atcgcctggc agcgcgccca cggccgcgac ggcctgccgt ggcagcgcac
     2761 gcgcgacccg taccgcgtgt ggctgtccga gatcatgctg cagcagacgc aggtcgcgac
     2821 cgtcctcggc tactacgcgc gcttcctcga gcgcttcccg accgtcgagg cgctggccgc
     2881 cgcgccgacc gaggacgtgc tcgcgctgtg ggccgggctc ggctactacg cccgggcgcg
     2941 caaccttcac cgttgcgcgc aggtggtggc gcgcgagcat ggcggtgcct tcccgcgcag
     3001 cgccgaggcg ctcgccgcgc tgcccggcat cggccgatcc acggccgccg cgatcgcctc
     3061 gttctgcttc ggcgagcgcg cggccatcct cgacggcaac gtcaagcgcg tgctcgcgcg
     3121 cgtgctggcg ttcgagcgcg acctcgcgca ggcggcgtcc gagaaggcgc tgtgggcgat
     3181 cgccgacggc ctgctgcccc acgccccggc cggcgagccg ctgcacgcgg cgatggcgtc
     3241 gtacacgcag ggcttgatgg atctcggcgc cacggtctgc acgatccgcc gcccggcttg
     3301 cgacgcctgc cccgcgcatg ggacgtgcgc cgggcgcgcg gcgggcgagc ccgagcgcta
     3361 cccggtcaag acgcgcaagc tcaagcgcgg caagcgcgcg ggcgtgctgc tgtggctgca
     3421 ggagcgcgat cgcgtctggc tggtgcgccg gccggacacg ggcgtgtggg cacagctgtg
     3481 gtcgctgccg gagttcgccg acgcggtcgc gctcgagcgc gcggtcgccg actggcccgg
     3541 cacgctcgaa tggctgccgc cgttcaagca cgtgttgacg cacttcgact ggatgctgca
     3601 gccactgcgc ctcgtctggc acggccgcgt ggacccgcag gaacagcgcg cgatcgacgc
     3661 gttgcgggcg ttcaacggcg gcgacggcag cgatcccggc gccgcgccga ccgctggccg
     3721 ctgggtcgcg cggtccgagc tcgactcggt gggtgtgccg gcgccgatca ggaagctgct
     3781 ggacgccggg ccttgagcat ggacgcgacg ctcatgaacg tgactgaccg tcaatcgacg
     3841 gcatccagct cccgatgccg cagcagcgtc gtcaccgcgc cgccgaagcg ctcggccagg
     3901 gtctcgcaca ggtagaccga gcggtgctgg ccgccggtgc agccgatcgc gacggtgagg
     3961 tagctgcgct ggtcgtcctc gaagctgggc agccagcggc gcaggaactg ctcgatctgg
     4021 cccatcatct cgccgacctc gggctgcgcg cgcaggtagt cggcgacggg cgcgtcgcgg
     4081 ccggtcaacg cgcgcagctc gcgcacgtag tacgggttgg gcaggatgcg gacgtcgaac
     4141 accatgtcgg cgtccagcgg cacgccgcgc ttgaacgcga acgactcgaa gaccagcgtg
     4201 agctggctcg catgcgcgcc gacgaggtcg cggatccagc gccgcagctg caccgggcgc
     4261 agctggctcg tgtcgaggat gctggagcgc atgcggatcg ctgcgagcag ttcgcgctcg
     4321 agctcgatgg cctcgatcag cgcgcggtgc tcgtcggtgg gcagcgtgcc cttggacagc
     4381 ggatgcgggc ggcgcgactc ggagtagcgg cgcacgagcg tgtcggtgcc ggcgtcgagg
     4441 aagaccggcc gaaccttcac gccgtcctcg cgcagctgct ccagcagcgg caccaggcgc
     4501 gggagcgagc cggcgctgcg cacgtccatc gcgacggcga tgcggccgat gccgcgttcg
     4561 cgctgcaggc gcaggaactc gggcagcagc tcgggcggca ggttgtcgca gcagaagaag
     4621 ccggcgtcct ccagcgcgtg cagcgcgatc gacttgcccg agcccgagat gcccgtgacc
     4681 agcacgacct cgcggatgcc gccttccgag gcgccgggcg cgggcacggg cgcgtcgtag
     4741 ggaatggctt cgccgatcgg gtcgttgaag tcgtcgggcg tcatgagcgg ggagcggtgc
     4801 ggccccgcgc ggcggcgggt gggatggcgg gtgccgcgtc gacggcgacg gcctgcgcga
     4861 tcagctcctg cgcgtgcgcg aggctcgtgc cggagctgcg ctcgccgccc agcatgcgcg
     4921 cgacctcggc cacgcgcgcc tcg
//
LOCUS       DASVLN010000867         4516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_43806, whole genome shotgun sequence.
ACCESSION   DASVLN010000867 DASVLN010000000
VERSION     DASVLN010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43806"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="VF453_20400"
     CDS             <1..1857
                     /locus_tag="VF453_20400"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7690079.1"
                     /translation="AAAGATLVHELALRQRDGTPLTLELAIRPIPRPRARGIPPNLLL
                     EGRDITLRKAAEDKLHLAAAVFEQAREGFVIADREGRVVTVNQAFCDISGYSADELQG
                     QQTAALGFGIGRREVRRQVRAALQASGHWQGEIRTSRKDGSLYTCWAAITQQRDAAGA
                     VTHYIGILNDITEFREVERNLVRLAHVDTLTDLPNRSLLADRVGQLTSVSRRDHRPFA
                     LMFMDLDQFKNINDTLGHSVGDALLAEVARRLQGSLRECDTVARLGGDEFAVLLPGAD
                     ATGAESVATKLLERLAEPCMVQGHELSMTMSIGIATYPSDGDDYEILSRNADTAMYRA
                     KHEGKATWRFFAAGMQQRSARQLQLESALRRALERNELLLHYQPQLAADGETLVGVEA
                     LLRWKHPEFGFVSPAEFIPLAETSGQIVAIGEWVLRTAVRQLKAWMDAGVPPMVVAVN
                     LSAVQFRHPQLPDVVQRALADAGLPAQYLELELTESVTANPAAAISMMDALHGLGVRL
                     SLDDFGTGYSSLSHLKRFPLHTLKIDQSFVRDIDTDPDDRAIVQAIIQMARALDLQTV
                     AEGVETDAQHQFLRREGCGMMQGYLFCRPMDVAALEMWIAAREQAAAALAAA"
     gene            complement(1972..2436)
                     /locus_tag="VF453_20405"
     CDS             complement(1972..2436)
                     /locus_tag="VF453_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7690080.1"
                     /translation="MKVSDILRVKGNTLFTVSPDQPLQEAVTTMAEHDIGSLVVMDLG
                     ELTGMLTFREVIRAVVRNGGQIGTTRVRSVMDDAPLTCTPETEIDEVRRMMLGRHARY
                     MPVLNGQTLMGVISFYDVARTVVESQDFENRMLKAYIRDWPSEEDVKVGQSL"
     gene            2607..3911
                     /locus_tag="VF453_20410"
     CDS             2607..3911
                     /locus_tag="VF453_20410"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX7690081.1"
                     /translation="MPGPSDPGFDTLALHAGAAPDPATGARATPLHLTTSFVFESSEH
                     AASLFNMERAGHVYSRLSNPTTAVFEERMAALEGGVGAVATASGQAALHLAIATIAGA
                     GAHVVASSALYGGSHNLLAYTLARFGIETTFVKPRELDAWRAAIRPTTRLLLGESLGN
                     PGLDVLDIPRVAEIAHGQGLPLLVDATFATPYLQQPFKLGADLVFHSATKFLSGHGTV
                     VGGVLVDSGRFDWERSGRFPELSQPYAGFHEMVFTEESTVGAFLLRARREGLRDFGAC
                     MSPHTAWLILQGLETLPLRMERHVANARKVVEFLAAHPMVARVGWPELESHPDHALAR
                     TLLPRGCGSVFSFDLKGSRAQGAAFIEALQLFSHLANVGDCRSLVIHPASTTHSRMDD
                     AALAAAGVGPGMIRLSIGLEDPADLCADLKSALKAAEKAGAR"
     gene            3908..>4516
                     /locus_tag="VF453_20415"
     CDS             3908..>4516
                     /locus_tag="VF453_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7690082.1"
                     /translation="MKFTVDGRTAWAYTGGKPWTPGMRTIVFVHGALHDHSVWTLPAR
                     WYAHHGVAALAVDLPGHGRSDGPPPASIEAAGDWLVALLEAAGAGPASLVGHSMGSLI
                     ALEAAARAPQRVEALVMVGTAYPMKVSAALLDTARDDPARAISMVNAFSISTTAAKPS
                     YPGPGAWLHGGNAALMHRIQDDAARAGTPNLFEHDFRVCDAYA"
BASE COUNT          639 a         1735 c         1514 g          628 t
ORIGIN      
        1 gccgcggccg gcgcgacgct ggtgcacgag ctcgccctgc gccagcgcga cggcaccccc
       61 ctcacgctgg agctggccat ccggccgatc ccgcggccgc gcgcgcgcgg catcccgccc
      121 aacctgctgc tcgaggggcg cgacatcacg ctgcgcaagg ccgccgagga caagctgcac
      181 ctggccgccg ccgtcttcga gcaggcgcgc gagggcttcg tgatcgccga ccgcgagggc
      241 cgcgtcgtca cggtcaacca ggccttctgc gacatcagcg gctactcggc cgacgagctc
      301 cagggccagc agaccgcggc gctgggcttc ggcatcggcc gccgcgaggt gcgccgccag
      361 gtgcgcgcgg cgctgcaggc ctcgggccac tggcagggcg agatccgcac ctcgcgcaag
      421 gacggcagcc tctacacctg ctgggccgcg atcacgcagc agcgcgacgc ggccggcgcc
      481 gtgacgcact acatcggcat cctcaacgac atcaccgagt tccgcgaggt ggagcgcaac
      541 ctcgtgcgcc tcgcccacgt cgacaccctc accgacctgc ccaaccgcag cctgctggcg
      601 gaccgcgtcg gccagctgac gagcgtctcg cgccgcgacc accggccgtt cgcgctgatg
      661 ttcatggacc tcgaccagtt caagaacatc aacgacacgc tgggccacag cgtcggcgac
      721 gcgctgctcg ccgaggtcgc gcgccgcctg cagggcagcc tgcgcgagtg cgacacggtc
      781 gcccgcctcg gcggcgacga gttcgccgtg ctgctgcccg gcgccgacgc gacgggcgcc
      841 gaatccgtcg ccaccaagct gctcgagcgc ctggccgagc cgtgcatggt ccagggccac
      901 gagctcagca tgacgatgtc catcggcatc gccacctacc cctccgacgg cgacgactac
      961 gagatcctct cgcgcaacgc ggacaccgcg atgtaccgcg ccaagcacga gggcaaggcc
     1021 acgtggcgct tcttcgcggc cggcatgcag cagcgctcgg cgcggcagct gcagctcgag
     1081 tcggccctgc ggcgcgcgct cgagcgcaac gagctgctgc tgcactacca gccccagctt
     1141 gccgccgacg gcgagacgct ggtgggcgtc gaggcgctgc tgcgctggaa gcatcccgaa
     1201 ttcggcttcg tctcccccgc cgagttcatc ccgctggccg agaccagcgg ccagatcgtc
     1261 gccatcggcg agtgggtgct gcgcaccgcg gtgcgccagc tcaaggcctg gatggatgcc
     1321 ggcgtgccgc ccatggtcgt cgcggtcaac ctctcggcgg tgcagttccg ccatccgcag
     1381 ctgcccgacg tcgtgcagcg cgcgctcgcc gacgccggcc tgcccgcgca gtacctcgag
     1441 ctggagctca ccgagagcgt caccgccaac ccggcggccg cgatctcgat gatggacgcg
     1501 ctgcacggcc tgggcgttcg gctgtcgctg gacgacttcg ggaccggcta ctcgtcgctg
     1561 agccacctca agcgcttccc gctgcacacg ctcaagatcg accagtcctt cgtgcgcgac
     1621 atcgacacgg atcccgacga ccgcgccatc gtgcaggcca tcatccagat ggcgcgcgcg
     1681 ctcgacctgc agaccgtcgc cgagggggtg gagaccgatg cccagcacca gttcctgcgc
     1741 cgcgagggct gcggcatgat gcagggctac ctgttctgcc ggccgatgga cgtcgccgcg
     1801 ctggagatgt ggatcgcggc gcgcgagcag gccgcggccg cgctggcagc ggcataggcg
     1861 cgcccgcgcg ctgcgcagcg accacgcgcg ccccgccctc cgacaggcct ccaaaaagcc
     1921 gacggcccgc tcgcttcgcg cgaacgggcc gtcgcgtttc cgggcgatcc gtcagaggct
     1981 ctggccgacc ttgacgtcct cttccgacgg ccagtcgcgg atgtaggcct tgagcatgcg
     2041 gttctcgaag tcctgcgact cgacgaccgt gcgggccacg tcgtagaacg agatcacgcc
     2101 catcagcgtc tgcccgttga gcacgggcat gtagcgcgcg tggcggccga gcatcatgcg
     2161 gcgcacctcg tcgatctcgg tctcgggggt gcaggtgagc ggcgcgtcgt ccatcaccga
     2221 gcgcacgcgc gtcgtgccga tctggccgcc gttgcgcacg accgcgcgga tgacctcgcg
     2281 gaaggtgagc atgccggtga gctcgcccag gtccatcacg accagggagc cgatgtcgtg
     2341 ctcggccatc gtcgtcaccg cttcctgcaa cggctggtcg ggcgagacgg tgaacagggt
     2401 attgcccttg acccgcagga tgtcgctgac tttcatggca acgcctccgt cgcttcagat
     2461 acgaaaagga atgaggtgtc caacatagca cacaatcgct gaaacgcatc gcggattcca
     2521 ccgcgccctc gcccccgatc accggggcta tggcgatgcc gcgacagatt gatccgagct
     2581 cacgaaacca ccttccgccc cacgccatgc ccggcccttc cgaccccgga ttcgacaccc
     2641 tcgccctgca cgccggcgcc gcgcccgacc cggccacggg cgcccgcgcg acgcccctgc
     2701 acctgaccac ctcgttcgtc ttcgagtcga gcgagcacgc ggcctcgctg ttcaacatgg
     2761 agcgcgccgg ccacgtctac agccggctgt ccaacccgac gaccgccgtc ttcgaggagc
     2821 ggatggccgc gctcgagggc ggcgtcggcg ccgtcgccac ggccagcggc caggcggcgc
     2881 tgcacctcgc gatcgcgacg atcgccggcg ctggggcgca cgtggtcgcg agcagcgcgc
     2941 tgtacggcgg ctcgcacaac ctgctcgcct acacgctcgc gcgcttcggc atcgagacca
     3001 cctttgtcaa gccgcgcgag ctcgacgcct ggcgcgccgc gatccgcccg acgacgcgcc
     3061 tgctgctggg cgagtcgctc ggcaaccccg gcctcgacgt cctcgacatc ccgcgcgtcg
     3121 ccgagatcgc gcacggccag ggcctgccgc tgctggtcga cgcgacgttc gcgacgccct
     3181 acctgcagca gccgttcaag ctcggcgccg acctcgtctt ccactcggcg accaagttcc
     3241 tctccggcca cggcaccgtc gtcggcggcg tgctggtgga ctccggccgc ttcgactggg
     3301 agcgcagcgg gcgcttcccg gagctgtcgc agccctacgc gggattccac gagatggtct
     3361 tcaccgagga gagcaccgtc ggcgcctttc tcctgcgcgc gcgccgcgaa ggcctgcgcg
     3421 acttcggcgc ctgcatgagc ccgcacacgg cctggctgat cctgcagggc ctggagacgc
     3481 tgccgctgcg catggagcgg cacgtcgcca acgcgcgcaa ggtcgtcgag ttcctcgccg
     3541 cgcatccgat ggtcgcgcgc gtcggctggc cggagctcga gtcgcatccc gaccacgcgc
     3601 tcgcgcggac gctgctgccg cgcggctgcg gctcggtctt cagcttcgac ctgaagggct
     3661 cgcgcgcgca gggcgcggcc ttcatcgagg cgctgcagct gttctcgcac ctcgccaacg
     3721 tcggcgactg ccgctcgctc gtgatccatc ccgcgtcgac cacgcattcg cgcatggacg
     3781 acgcggcgct cgccgccgcg ggcgtcggtc cggggatgat ccggctgtcg atcggcctcg
     3841 aggatccggc cgacctgtgc gccgacctga agtccgccct gaaggcggcg gaaaaggcgg
     3901 gcgcgcgatg aaattcaccg tcgatggccg caccgcctgg gcctacaccg gcggcaagcc
     3961 ctggacgccg ggcatgcgca cgatcgtctt cgtccacggc gcgctgcacg accacagcgt
     4021 ctggacgctg cccgcacgct ggtacgcgca tcacggcgtc gccgcgctgg cggtcgacct
     4081 gccgggccac ggccgctcgg acggcccgcc accggcgtcc atcgaggcgg cgggcgactg
     4141 gctcgtcgcc ctgctcgagg ccgcgggcgc cgggccggcc agcctcgtcg gccacagcat
     4201 gggctcgctg atcgcgctcg aggcggccgc gcgtgcgccg cagcgcgtcg aggcgctggt
     4261 gatggtcggc acggcgtatc cgatgaaggt gagcgcggcc ctgctcgaca ccgcccgcga
     4321 cgacccggcg cgcgcgatct ccatggtcaa cgcgttctcg atctcgacca ccgcggccaa
     4381 gccgtcctac cccggccccg gggcgtggct gcacggcggc aacgcggccc tgatgcaccg
     4441 catccaggac gacgcggcgc gcgcgggcac gcccaacctc ttcgagcacg acttccgcgt
     4501 ctgcgacgcc tacgcc
//
LOCUS       DASVLN010000868         5557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_206313, whole genome shotgun sequence.
ACCESSION   DASVLN010000868 DASVLN010000000
VERSION     DASVLN010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206313"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /gene="pdxA"
                     /locus_tag="VF453_20420"
     CDS             <1..718
                     /gene="pdxA"
                     /locus_tag="VF453_20420"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX7690083.1"
                     /translation="LRCIEQAVAWTRAGAGRAIVTAPIHKEAFAAAGAHWPGHTEMLQ
                     ELAAAPGTAPPPVRMMLANEELRVVLVTIHLSLRRAIDALSVDGILETLRIADDALRR
                     FGLAAPRIGVAGLNPHAGEGGLFGDEEIRLIAPAIAQARAAGIDARGPFAPDTIFMRA
                     RDTAARAGEFDAVVAMTHDHGLIPVKYLGVEQGVNVTLGLPFVRTSPDHGTAFDIAGR
                     GIADPASLEAAVRMARRLAA"
     gene            complement(771..1187)
                     /gene="mscL"
                     /locus_tag="VF453_20425"
     CDS             complement(771..1187)
                     /gene="mscL"
                     /locus_tag="VF453_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX7690084.1"
                     /translation="MGFATEFKEFAVKGNVMDLAVGVIIGAAFGKIVDSVVNDLIMPL
                     VGKATGGLDFSNYFIPLAGQTATNLADAKKAGAVFAYGNFLTVALNFVILAFIIFLMV
                     KQINRLKHETPPPVPAPAPTPEDVVLLREIRDALKK"
     gene            1436..2050
                     /gene="petA"
                     /locus_tag="VF453_20430"
     CDS             1436..2050
                     /gene="petA"
                     /locus_tag="VF453_20430"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX7690085.1"
                     /translation="MSDSLVVQDGVDESKRTWVAITCGAGAIAGVATAVPFVSTFAPS
                     ERAKAAGAPVEVDISDVKPGEKKTVEWRGQPVWILRRTPEMIASLAKDEPELADPDSK
                     RNPDEQTPPYARNRTRSIKPEVLVVVGICTHLGCSPGDKFTPGAQPSLPSDWPGGWLC
                     PCHGSTFDLAGRVFKNKPAPDNLRVPPHQYLSDTKLLIGEDRKG"
     gene            2110..3510
                     /locus_tag="VF453_20435"
     CDS             2110..3510
                     /locus_tag="VF453_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEX7690086.1"
                     /translation="MAEFKVAPPNASTGVKLLTWVDNRFPATKLWKEHLSEYYAPKNF
                     NFWYFFGSLALLVLVIQIVTGIFLVMHYKPDANLAFASVEYIMRDVPWGWLVRYMHST
                     GASAFFIVVYLHMFRGLLYGSYRKPRELVWIFGCAIFLCLMGEAFMGYLLPWGQMSYW
                     GAQVIVNLFSAIPYIGPDLSLMIRGDYVVGDATLNRFFSFHVIAIPLILLGLVVAHVI
                     ALHEVGSNNPDGIEIKANKDANGIPRDGIPFHPYYSVHDLMGVAGFLIVFSAVVFFAP
                     EMGGYFLEPNNFIPADSLKTPPHIAPVWYFTPFYSMLRATTDEMKPVFAVAIAIAAVL
                     GLTKGRFGGGAKVAMLIAAALVMVGLFRIDAKFWGVVVMGSSVVILFFMPWLDRSPVK
                     SIRYRPGWHKAVYGVFVVTFLVLGYLGIQPPDPVKNIVSQVGTVLYFSFFLLMPWWSQ
                     LGSFKPVPDRVVFHAH"
     gene            3540..4310
                     /locus_tag="VF453_20440"
     CDS             3540..4310
                     /locus_tag="VF453_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX7690087.1"
                     /translation="MKRFIASVLVAIAALAGATPALAQEGGPAWDRFPAERVTDLAAL
                     QNGARLFVNYCLNCHQAAYMRYNRLHDIGLSDDQIKSNLLFTGEKVGDLMKTPLDPKD
                     AKAFFGTVPPDLSVIARSRSGPNGSGADYLYTYLRSFYRDETRPTGWNNRVFPNVGMP
                     HVLWELQGTQRPVYEDEKGEGGEVEHVFKGFRLETPGTLSAADYNADVADLVAYLQWM
                     SEPAQNFRVKLGVWVLIFLGVFWVICWRLNASYWKDVR"
     gene            4405..5019
                     /locus_tag="VF453_20445"
     CDS             4405..5019
                     /locus_tag="VF453_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7690088.1"
                     /translation="MMVLYSGTTDPYSHRCRFVLFEKGMDFEIRDVDLFAKPEDIALM
                     NPYNEVPILVERDLILYESHIINEYIDERFPHPQLMPGDPVARARVRLFLFNFEKELF
                     SNVNILEGRGGVKANDKQLEKARAQIRDRLTQLAPIFLKNKYMLGDDFSMLDVAIAPL
                     LWRIDYYGIELSKNAAPLLKYAERIFSRPAYIEALTPSEKVMRK"
     gene            5120..>5557
                     /locus_tag="VF453_20450"
     CDS             5120..>5557
                     /locus_tag="VF453_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEX7690089.1"
                     /translation="MTQVTTEIPGSSTRPYLIRALHDWCTDNGFTPYLAVHVDRSVQV
                     PMEYVKNNEIVLNVGFEATSGLKLGNDYIEFRARFGGMARDILVPVDHVVAIYARENG
                     QGMAFPMPTPVEGDAPAAAPEPAADGAPRGLRLAPTTTSATDAA"
BASE COUNT          880 a         1931 c         1808 g          938 t
ORIGIN      
        1 gctgcgctgc atcgagcagg ccgtcgcctg gacgcgcgcg ggcgcgggcc gcgcgatcgt
       61 caccgcgccg atccacaagg aggccttcgc cgccgccggc gcgcactggc cgggccacac
      121 ggagatgctg caggaactcg cggccgcgcc cggcaccgcg ccgccgccgg tacgcatgat
      181 gctcgccaac gaggagctgc gcgtcgtgct ggtgacgatc cacctgtcgc tgcggcgcgc
      241 gatcgacgcg ctgtcggtgg acggcatcct cgagacgctg cgcatcgcgg acgacgcgct
      301 gcgccgcttc ggcctcgcgg cgccgcgcat cggcgtcgcc ggactgaacc cgcacgcggg
      361 cgagggcggg ctgttcggcg acgaggagat ccgcctgatc gcgcccgcca tcgcgcaggc
      421 gcgcgccgcc ggcatcgacg cgcgcgggcc gttcgcgccc gacacgatct tcatgcgcgc
      481 ccgcgacacg gcggcgcgcg cgggcgagtt cgacgcggtc gtcgcgatga cgcacgacca
      541 cggcctcatc ccggtgaagt acctgggcgt ggagcagggc gtcaacgtga cgctgggact
      601 gcccttcgtg cgcaccagcc ccgaccacgg caccgcgttc gacatcgcgg gccgcggcat
      661 cgccgatccc gcgagcctcg aggccgcggt gcgcatggcg cgccggctgg ccgcctagat
      721 gtgcccccgc cacggccctg ccggcatgct cgccggccgg gccggcgtgc tcattttttc
      781 agcgcgtcgc ggatctcgcg cagcagcacc acgtcctccg gcgtcggggc gggcgccggc
      841 acgggcggcg gcgtctcgtg cttgagccgg ttgatctgct tgaccatcag gaaaatgatg
      901 aacgcgagga tgacgaagtt cagcgccacg gtcaggaagt tgccgtacgc gaagaccgcg
      961 cccgccttct tcgcgtcggc caggttggtg gccgtctgcc ccgcgagcgg gatgaagtag
     1021 ttcgagaagt cgaggccccc ggtggccttg cccacgagcg gcatgatgag gtcgttcacg
     1081 accgaatcga cgatcttgcc gaacgccgcg ccgatgatga cgccgacggc cagatccatc
     1141 acgttgccct tgacggcgaa ttccttgaac tcggttgcga atcccatgtg cgactccttg
     1201 cttattggtt gatccaggtt gaccgggccc gccggcggaa gccggatact cccgccggtg
     1261 ggccggtgcg gcagtattgc gccgccacgg aatgcgtcaa tcgcccggtc gctgccatgt
     1321 gcggcagttc gcgtaccgag ggtttgcctg tggtgcgcgg gtcgcttcaa ggggacgagt
     1381 cgcacgctaa actccagcgt tgacttgatc agttactttc ggctcgagga cgttcatgag
     1441 tgatagcctt gtagtgcagg atggagttga tgagagcaag cggacctggg tcgcgatcac
     1501 gtgcggcgcc ggggccatcg cgggagtcgc gacggcggtt cccttcgtga gcaccttcgc
     1561 tccctcggag cgggccaagg cggccggtgc gccggtggag gtcgacatct ccgacgtcaa
     1621 gccgggcgag aagaagacgg tcgaatggcg cggccagccg gtgtggatcc tgcgccggac
     1681 cccggagatg atcgcctccc tcgccaagga cgagccggaa ctggcggacc cggactccaa
     1741 gcgcaacccc gatgagcaga cgccgccgta cgcgcgcaac cgcacccgct cgatcaagcc
     1801 ggaggtgctg gtggtcgtgg gcatctgcac gcacctgggc tgctcgccgg gcgacaagtt
     1861 cactcccggc gcccagccct cgctgccctc ggactggcct ggcggctggc tgtgcccgtg
     1921 ccacggctcg acgttcgatc tcgccggccg cgtgttcaag aacaagccgg cgccggacaa
     1981 cctgcgcgtg ccgccgcacc agtacctgtc cgacacgaag ctgctgatcg gcgaggacag
     2041 gaaaggctga ggcccgcggg ccgccccttt cgcccagccc ccgccgccgc accaagacac
     2101 gaggaccaca tggccgaatt caaggtagct cccccgaatg cctccacggg cgtcaagctg
     2161 ctcacctggg tcgacaaccg cttccccgcg accaagctct ggaaggagca cctgtccgag
     2221 tactacgcgc cgaagaactt caacttctgg tacttcttcg gctcgctggc gctgctggtg
     2281 ctggttatcc agatcgtcac cggcatcttc ctggtgatgc actacaagcc cgacgcgaac
     2341 ctggcgttcg cctcggtcga gtacatcatg cgcgacgtcc cctggggctg gctggtgcgc
     2401 tacatgcact cgaccggcgc atccgcgttc ttcatcgtcg tctacctgca catgttccgc
     2461 gggctgctgt acggctcgta ccgcaagccg cgcgagctgg tctggatctt cggctgcgcg
     2521 atcttcctgt gcctgatggg cgaggccttc atgggctacc tgctgccctg gggccagatg
     2581 tcgtactggg gcgcgcaggt gatcgtgaac ctgttctccg cgatccccta catcggcccg
     2641 gacctctcgc tgatgatccg cggcgactac gtcgtcggcg acgcgacgct gaaccgcttc
     2701 ttcagcttcc acgtgatcgc gatcccgctg atcctgctcg ggctggtcgt cgcgcacgtc
     2761 atcgcgctgc acgaagtggg ctcgaacaac ccggacggca tcgagatcaa ggcgaacaag
     2821 gacgccaacg ggatcccgcg cgacggcatc ccgttccacc cctactactc ggtgcacgac
     2881 ctgatgggcg tggcgggctt cctgatcgtg ttcagcgcgg tcgtgttctt cgcgccggag
     2941 atgggcggct acttcctcga gccgaacaac ttcatccccg ccgactcgct gaagacgccg
     3001 ccgcacatcg cgccggtctg gtacttcacg ccgttctact cgatgctgcg cgcgacgacc
     3061 gacgagatga agccggtctt cgcggtcgcg atcgcgatcg ccgcagtgct cggactgacc
     3121 aagggcaggt tcggcggcgg cgcgaaggtg gcaatgctga tcgcggccgc gctggtgatg
     3181 gtcggcctgt tccgcatcga tgccaagttc tggggcgtgg tggtgatggg cagctcggtc
     3241 gtgatcctgt tcttcatgcc ctggctcgac cgcagccccg tgaagtcgat ccgctaccgt
     3301 ccgggctggc acaaggccgt gtatggggtg ttcgtggtga ccttcctggt gctcggctac
     3361 ctgggcatcc agccgcccga tccggtgaag aacatcgtct cgcaggtcgg caccgtcctg
     3421 tacttcagct ttttcctgct gatgccgtgg tggagccagc tgggcagctt caagcccgtg
     3481 ccggatcgcg tcgtcttcca cgcgcactga tcgccatgca gaagaaggaa acgagcctca
     3541 tgaagcgttt catcgcctcc gtcctcgtcg ccatcgccgc gctggccggg gccacccccg
     3601 cgctcgcgca ggaaggcggc ccggcctggg accggttccc ggccgagcgc gtgaccgatc
     3661 tcgcggccct gcagaacggc gcgcggctgt tcgtcaacta ctgcctgaac tgccaccagg
     3721 ccgcgtacat gcggtacaac cgcctgcacg acatcgggct gtccgacgat cagataaaat
     3781 ccaacctgct gttcacgggc gagaaggtcg gcgacctgat gaagacgccg ctggatccca
     3841 aggatgccaa ggccttcttc ggcaccgtcc cgcccgacct gtccgtgatc gcacgctccc
     3901 gttcagggcc gaacggaagc ggcgcggatt acctctacac ctacctccgg tctttctacc
     3961 gggacgagac gaggcccacg ggatggaata atcgcgtctt cccgaacgtc ggcatgccgc
     4021 acgtgctgtg ggagctgcaa ggcacccagc gcccggtcta tgaggacgag aagggagaag
     4081 gcggagaggt cgagcacgtg ttcaagggct tcaggctcga gaccccgggc acgctcagcg
     4141 ccgccgatta caacgccgac gtcgccgatc tcgttgcgta cctgcaatgg atgagcgaac
     4201 cggcgcagaa cttccgcgtg aagctgggcg tctgggtgct catcttcctg ggggtgttct
     4261 gggtgatctg ctggcggctc aatgcctcgt actggaagga cgtcagatag cgtccgaccg
     4321 ggctccgctc ccgaggcgcg cacccgaccg gcttgccggt cggcttgttt ttttgacgac
     4381 tgacttttcg aggatgcccc cgccatgatg gtgctgtatt caggaaccac cgacccgtac
     4441 tcgcaccgct gccgcttcgt gctgttcgag aagggcatgg acttcgagat ccgcgacgtg
     4501 gatctgttcg ccaagcctga agacatcgcg ctgatgaacc cgtacaacga ggtgcccatc
     4561 ctcgtcgagc gcgacctgat cctgtacgag tcgcacatca tcaacgagta catcgacgag
     4621 cgcttcccgc atccgcagct gatgcccggc gacccggtcg cccgcgcccg cgtgcgcctg
     4681 ttcctgttca acttcgagaa ggaactgttc tcgaacgtca acatcctgga aggccgcggc
     4741 ggcgtgaagg ccaacgacaa gcagctcgag aaggcgcgcg cccagatccg cgaccgcctg
     4801 acccagctcg cgccgatctt cctgaagaac aagtacatgc tcggcgacga cttctcgatg
     4861 ctcgacgtcg cgatcgcgcc gctgctgtgg cgcatcgact actacggcat cgagctgtcg
     4921 aagaacgccg ccccgctgct gaagtatgcc gagcgcatct tctcgcgccc ggcctacatc
     4981 gaggcgctga cgccttcgga aaaggtgatg cgcaagtgac gccggcgcgg tcggccgggc
     5041 tgtcgcggcc ggccggccgt cggcgtgcga tgatcgcgtc gcccgccgcc tccgcatgcc
     5101 agcccgccct ccgccgtcca tgacccaggt caccaccgag attcccggca gctccacccg
     5161 gccctacctg atccgtgcgc tgcacgactg gtgcaccgac aacggcttca cgccctacct
     5221 cgccgtgcac gtcgaccgca gcgtccaggt gccgatggag tacgtgaaga acaacgagat
     5281 cgtgctcaac gtcggcttcg aggcgaccag cggcctgaag ctcggcaacg actacatcga
     5341 gttccgcgcc cgcttcggcg gcatggcgcg cgacatcctc gtgcccgtcg accacgtcgt
     5401 cgcgatctac gcacgcgaga acgggcaggg catggcgttc ccgatgccga cgccggtcga
     5461 gggcgacgct ccggccgccg cgcccgagcc cgctgccgat ggcgcccccc gcggcctgcg
     5521 cctggcgccg acgaccacct ccgcgacgga cgcggca
//
LOCUS       DASVLN010000869         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_206341, whole genome shotgun sequence.
ACCESSION   DASVLN010000869 DASVLN010000000
VERSION     DASVLN010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206341"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..484)
                     /locus_tag="VF453_20455"
     CDS             complement(320..484)
                     /locus_tag="VF453_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690090.1"
                     /translation="MTKLLAAALISMFAVGAYAADAASAPAAAPAKKAKAAHHKAAKK
                     AEGAASAASK"
     gene            655..1908
                     /gene="icd"
                     /locus_tag="VF453_20460"
     CDS             655..1908
                     /gene="icd"
                     /locus_tag="VF453_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX7690091.1"
                     /translation="MSEHVKVPAGGQKITVNADFSLNVPDQPIIPFIEGDGTGADITP
                     VMIKVVDAAVAKCYGDKRRIHWMEVYAGEKSTKVYGPDVWLPEETLKFVRDYVVSIKG
                     PLTTPVGGGIRSLNVALRQELDLYVCLRPVQYFKGVPSPVKAPEKVDMVIFRENSEDI
                     YAGIEYEAESDKARKVIDFLINEMGVKKIRFPNTSGIGIKPVSREGTERLVRKAIQYA
                     IDNDKPSVTLVHKGNIMKFTEGGFRDWGYALAQREFGAELIDGGPWCKFKNPKTGKEI
                     TIKDSIADAFLQQIILRPAEYSVIATLNLNGDYISDALAAQVGGIGIAPGANLSDSVA
                     MFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWTEAADLIIAAMERAILSKKVTY
                     DFARLMDGATQVSCSGFGQVMIDHM"
     gene            2117..>2323
                     /locus_tag="VF453_20465"
     CDS             2117..>2323
                     /locus_tag="VF453_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690092.1"
                     /translation="MKFTPSFVLAAVSAALLAACGGSGGDGSGGSSQPAAVAISSGNQ
                     TDVARATVSGGMTVSLAGGGANGAS"
BASE COUNT          406 a          779 c          793 g          345 t
ORIGIN      
        1 ggtcgcgctc ggagcgcacg cgctggccga cgcctcggcc ttcgaccggg tcatcgcgat
       61 cgtcggccag tcgcccgagc agcgcgaggc cggacgccag cgctggcgcg agtacgagca
      121 cgccggccac gccatgacgc acgtggcggc ggccggcggc ggcgccgaag gctgatcgtc
      181 ccggccaggc gccgggcggg gcggaaacga cgaggcccgc acgcggcggg cctggaatga
      241 aaaacgcccg gccggggccg ggcgtcttcc gctcgaaccg cgccggacag caccggctcg
      301 gctcgggcgc gattccaaat tacttggaag cggcggaggc ggcgccttcg gccttcttgg
      361 cggccttgtg gtgggcggcc ttggccttct tggccggggc ggcggcgggg gccgaagcgg
      421 cgtcggcggc gtaggcgcca acggcgaaca tggagatcag ggcggcggcc aggagcttgg
      481 tcattttcag gtaccttgaa aattgaagtt gaaagtgctt tcccgtcagg gcatagccca
      541 gctccaacgc aacacccttc cgggcggttg acaccttttc gatgaactag aatctttttt
      601 tccccggacg ccccggaacg cgtccaaccc cgagaacagc gtggagcaat ttcaatgagc
      661 gaacatgtca aggtgcccgc gggcggccag aagatcacgg tcaacgccga cttctccctg
      721 aacgtgcccg accagccgat catccccttc atcgagggtg acggcacggg cgcggacatc
      781 acgcccgtga tgatcaaggt cgtcgacgcg gcggtcgcca agtgctatgg cgacaagcgc
      841 cggatccact ggatggaggt ctatgccggc gagaagtcga ccaaggtcta cggcccggac
      901 gtctggctgc cggaggagac gctgaagttc gtgcgcgact acgtggtctc gatcaagggc
      961 ccgctgacga cgccggtggg cggcggcatc cgctcgctca acgtcgccct gcgccaggag
     1021 ctcgacctct acgtctgcct gcgcccggtg cagtacttca agggcgtgcc ctcgccggtc
     1081 aaggcgcccg agaaggtcga catggtcatc ttccgcgaga actccgagga catctacgcc
     1141 ggcatcgagt acgaggcgga gagcgacaag gccaggaagg tcatcgactt cctgatcaat
     1201 gagatgggcg tcaagaagat ccgcttcccg aacacctcgg gcatcggcat caagcccgtg
     1261 tcgcgcgaag gcaccgagcg cctggtgcgc aaggcgatcc agtacgcgat cgacaacgac
     1321 aagccctccg tgacgctggt tcacaagggc aacatcatga agttcacgga aggcggcttc
     1381 cgcgactggg gttacgcgct cgcccagcgc gagttcggcg ccgagctgat cgacggcggc
     1441 ccgtggtgca agttcaagaa cccgaagacg ggcaaggaaa tcacgatcaa ggactcgatc
     1501 gccgacgcct tcctgcagca gatcatcctg cgcccggcgg agtactcggt catcgcgacg
     1561 ctgaacctca acggcgacta catctccgac gcgctggccg cgcaggtggg cggcatcggc
     1621 atcgccccgg gcgccaacct gtcggactcg gtcgcgatgt tcgaggccac ccacggcacc
     1681 gccccgaagt acgccggcaa ggactacgtc aaccccggct ccgaaatcct gtcggccgag
     1741 atgatgctgc gccacatggg ctggacggaa gcggccgacc tgatcatcgc cgcgatggag
     1801 cgcgccatcc tgagcaagaa ggtcacctac gacttcgccc gcctgatgga cggcgccacg
     1861 caggtctcgt gctcgggctt cggccaggtc atgatcgacc acatgtgagg acgtgcccgg
     1921 tcgcgaaccg ggttgccctg acgaacccca ggtggtcgag acccctgggg ttttttgttg
     1981 cgccgtcatc ggcgggcgac ggccggcgac cacccgtcgg ggggagttgg ccgcaggcga
     2041 tccgtgctag cgttcgccgt tcgcccgcaa gccgtgcggg tgacgcatcg acacacagct
     2101 caggagggag cgccccatga agttcacccc atcgttcgtc ctagccgccg tgtccgcggc
     2161 cctgctcgcc gcgtgcggcg gctccggtgg cgacggctcc ggcggcagct cgcagccggc
     2221 cgcggtcgcg atcagcagcg gcaaccagac cgacgtcgct cgcgccacgg tgtccggcgg
     2281 catgaccgtg tcgctcgccg gcggcggtgc caacggcgcg agc
//
LOCUS       DASVLN010000870         4034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_43859, whole genome shotgun sequence.
ACCESSION   DASVLN010000870 DASVLN010000000
VERSION     DASVLN010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43859"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF453_20470"
     CDS             complement(<1..308)
                     /locus_tag="VF453_20470"
                     /inference="COORDINATES: protein motif:HMM:NF016494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HEX7690093.1"
                     /translation="MTSVRLGDIVAALGGELRGDPDHAIARIAPLENAAGDEITFVAQ
                     ARFKAQLEASAAGAVVLSPALVEAAPAGCRLVVVDDPYLYFARLTQWWARRTRPAPAA
                     G"
     gene            complement(328..825)
                     /locus_tag="VF453_20475"
     CDS             complement(328..825)
                     /locus_tag="VF453_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX7690094.1"
                     /translation="MKWLLALVLSAVSFAVAAQQLKIGYVDSGRVLREADMAKRATQR
                     LEAEFSKRQKDLNDQKARLDAASDKLDKDAPTLSDSEKTRRQRELVEQDRDLQRKQRE
                     FQEDLTQRRNEEVASVIERATRTIKQIGDTDHYDVILSDQVAIYSSPRVDITQKVIDA
                     LNASK"
     gene            complement(840..3122)
                     /gene="bamA"
                     /locus_tag="VF453_20480"
     CDS             complement(840..3122)
                     /gene="bamA"
                     /locus_tag="VF453_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX7690095.1"
                     /translation="MRPVAACVVLAGAVLPGAALAIDPFTIRDIRVEGLQRTDPGSVF
                     SVLPFRVGDSYTDDKAAAALRALFATGLFKDVRIDVEGDVVVVIVDERATIASVNFLG
                     TKEFDKDTLTKALKDAGIGEGLPFDKALADRAEQEIKRQYLGRSLYAAEVVTTVTPLE
                     RNRVNITFKVTEGDVAHIRDIHIIGSKAFSESKLIDQMDLTTGGWLTWYTKSDRYSRT
                     KLNADLEKLRSFYLNRGYLEFDITSTQVTISPDKQTISITITVSEGQPYVVTGVRLEG
                     NYLGREAEFKNLVTIKPGAAYHGDDVTATVKAFTDLYGTYGYAFAHVEPHPEIDRKTG
                     QVVVVLQSDPGRRAYVRRVNLAGNTITRDEVIRREFRQFESAWYDGGKIKLSRDRVDR
                     LGFFSSVDVDTNDVPGAPDQVDLTLNVVEKPTGNLTLAAGYSQTDKLSITGSVRKENV
                     FGSGNYLSLDVSTAHTNKNLSISETDPYFTDDGISRTIDLYFRTSRPYNSLGTEYAVS
                     TPGAAIRFGLPYSEFDTVFVGVGAEQTHIGTSAGVPDSYVIFVDKFGSYANSFPMTLG
                     WSRDERDSGISPTSGRFARFNADFSLFGDVQYARLNSQVTQYIDLGNHFSLGINAEVG
                     YGFGLNGKPYPVFKNFYGGGLGSVRVFEQGSLGKGSVDVTGAFIGGSKKMNINNELYI
                     PVPGAANDKSLRLFAFVDAGNVWAEDEHIALGSLRASAGLGLSWTSPVGPFKLSYGLP
                     IKSEPNDRIQKFQFQVGTAF"
     gene            complement(3154..>4034)
                     /locus_tag="VF453_20485"
     CDS             complement(3154..>4034)
                     /locus_tag="VF453_20485"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7690096.1"
                     /translation="SDEDIYENVAVALMDQRPLLLRVRAPDGAGERTLTLPLPSLGKT
                     EIDADVFARLGLGVYVRPVLGRVQADGPAARAGLRKGDLVTAIDGHPIADRNELLQRI
                     RGSAADGQAHPLQVQVLRAGEPVSLVVTPLVVADGDRHFGRVEAEVGEPPAQQLVRDG
                     LGASLWFGVAQTWDRAGLQVRLFGRMLTGRMSVKNLSGPISIADAAEQSISHGFMDFV
                     LFLAEISVGLGVLNLLPLPVLDGGRLLYYLFEGATGRPVSALWQTRLQIGGVLAILLL
                     MSLALSNDVARHLGQQ"
BASE COUNT          617 a         1347 c         1406 g          664 t
ORIGIN      
        1 cccgcggccg gcgccggacg cgtgcgcctt gcccaccact gcgtgaggcg ggcgaagtac
       61 agatagggat cgtcgacgac caccaggcgg cagccggccg gcgcggcctc gaccagcgcg
      121 ggcgacagca ccacggcgcc ggcggcgctc gcctccagct gggccttgaa gcgcgcctgc
      181 gcgacgaagg tgatctcgtc gcccgccgcg ttctcgagcg gcgcgatgcg cgcgatcgcg
      241 tggtcggggt cgccccgcag ctcgccgccc agcgccgcaa cgatgtcgcc gaggcgaacc
      301 gaagtcatat cgcgggcccg ggcgcggtca cttggacgcg ttcagcgcgt cgatcacctt
      361 ctgcgtgatg tcgacccggg ggctggagta gatcgccacc tggtcgctca ggatcacgtc
      421 gtagtggtcg gtgtcgccga tctgcttgat cgtgcgcgtg gcgcgctcga tcacgctggc
      481 gacttcctcg ttgcggcgct gcgtcaggtc ctcctggaac tcgcgctgct tgcgctggag
      541 gtcgcggtcc tgctcgacga gctcgcgctg gcgacgcgtc ttctcgctgt ccgacagcgt
      601 cggcgcgtcc ttgtccagct tgtcgctcgc ggcgtccagg cgcgccttct ggtcgttcag
      661 gtccttctgg cgcttgctga actccgcctc cagccgctgc gtcgcgcgct tggccatgtc
      721 ggcctcgcgc agcacgcgcc cactgtccac gtagccgatc ttcaactgct gcgcggcgac
      781 cgcgaacgag accgccgaca agaccagggc gagcaaccat ttcatggaac ggactttcat
      841 cagaacgcgg taccgacttg gaattggaac ttctggattc tatcgttcgg ttcggacttg
      901 atcggcaggc cgtagctcag cttgaagggg cccaccggcg aggtccagct caggcccagg
      961 ccggccgagg cgcgcagcga gcccagcgcg atgtgctcgt cctcggccca cacgttgccg
     1021 gcgtcgacga acgcgaacag ccgcaggctc ttgtcgttgg ctgcgccagg caccgggatg
     1081 tagagctcgt tgttgatgtt catcttcttg gagccaccga tgaacgcgcc ggtcacgtcg
     1141 accgagccct tgcccagcga gccctgctcg aacacgcgca ccgagccgag gccgccgccg
     1201 tagaagttct tgaacaccgg gtacggcttg ccgttgaggc cgaagccgta gccgacctcc
     1261 gcgttgatgc ccagcgagaa gtggttgccg aggtcgatgt actgcgtcac ctgcgagttc
     1321 aggcgcgcgt actgcacgtc gccgaacagg ctgaagtcgg cgttgaagcg cgcgaagcgg
     1381 ccgctggtgg gcgagatgcc gctgtcgcgc tcgtcgcgcg accagcccag ggtcatcggg
     1441 aacgaattcg cgtacgagcc gaacttgtcg acgaagatca cgtagctgtc gggcacgccg
     1501 gccgaggtgc cgatgtgcgt ctgctccgcg ccgacgccga cgaagaccgt gtcgaactcc
     1561 gagtacggca ggccgaagcg gatcgccgcg ccgggcgtcg agaccgcgta ctcggtgccc
     1621 aggctgttgt acgggcgcga ggtgcggaag tagaggtcga tcgtgcgcga gatgccgtcg
     1681 tcggtgaagt acggatcggt ctcgctgatg gacaggttct tgttcgtgtg ggccgtgctc
     1741 acgtccagcg acaggtagtt gccggagccg aacacgttct ccttgcggac cgagcccgtg
     1801 atcgagagct tgtcggtctg cgagtagccc gccgccagcg tcaggttgcc ggtcggcttc
     1861 tcgacgacgt tgagggtcag gtccacctgg tcgggcgccc cggggacgtc gttggtgtcg
     1921 acgtcgacgc tgctgaagaa gccgaggcgg tcgacacggt cgcgcgagag cttgatcttg
     1981 ccgccgtcgt accaggccga ctcgaactgg cggaactcgc gacggatcac ctcgtcgcgc
     2041 gtgatcgtgt tgcccgcgag gttgacgcgg cgcacgtagg cgcggcggcc ggggtcggac
     2101 tgcagcacga cgaccacctg gccggtcttg cggtcgatct ccggatgcgg ctcgacgtgc
     2161 gcgaacgcgt agccgtaggt gccatacagg tcggtgaagg ccttgaccgt ggccgtgacg
     2221 tcgtcgccgt ggtaggccgc gccgggcttg atcgtgacga ggttcttgaa ctcggcctcg
     2281 cggccgaggt agttgccctc caggcgcacg ccggtgacga cgtagggctg gccctcgctc
     2341 accgtgatcg tgatcgagat cgtctgcttg tcgggcgaga tcgtgacctg cgtcgacgtg
     2401 atgtcgaatt ccaggtagcc gcggttcagg tagaacgagc gcagcttctc gaggtcggcg
     2461 ttgagcttgg tgcgcgaata gcggtcggac ttggtgtacc aggtcagcca gccgccggtc
     2521 gtcaggtcca tctggtcgat cagcttggac tccgagaacg ccttgctgcc gatgatgtgg
     2581 atgtcgcgga tgtgggcgac gtcgccctcg gtcaccttga acgtgatgtt gacccggttg
     2641 cgctccagcg gcgtgacggt cgtgacgacc tccgccgcgt acaggctgcg acccaggtac
     2701 tggcgcttga tctcctgctc cgcgcggtcg gccagcgcct tgtcgaacgg caggccctcg
     2761 ccgatgccgg cgtccttcag cgccttcgtg agcgtgtcct tgtcgaactc cttcgtgccc
     2821 aggaagttca ccgacgcgat cgtggcgcgc tcgtcgacga tgacgaccac cacgtcgccc
     2881 tcgacatcga tgcgcacgtc cttgaacagg ccggtcgcga acagcgcgcg cagcgccgcg
     2941 gcggccttgt cgtcggtgta gctgtcgccg acgcggaacg gcagcaccga gaacacgctg
     3001 cccggatccg tccgctgcag gccttcgacg cggatgtcgc ggatcgtgaa cggatcgatc
     3061 gccagtgcgg ctccaggcag aacggcgccc gcgaggacca cgcaggcagc gacagggcgc
     3121 atcaggctcg ggaagagaga agacatcggg tgtttattgc tggcccaggt ggcgagccac
     3181 gtcgttggaa agggcgagag acatcagcag caggatggcg agaaccccgc cgatctgcag
     3241 ccgggtctgc catagcgcgg agaccgggcg gccggtcgca ccctcgaaga gataatacag
     3301 caggcgcccc ccatcgagga cgggcagcgg cagcaggttc aggacgccga gacccacgct
     3361 gatctcggcg aggaacagca cgaagtccat gaagccgtgg ctgatcgact gctccgcggc
     3421 gtcggcgatc gagatcggtc cggacaggtt cttcaccgac atccggcccg tcagcatgcg
     3481 gccgaacagg cgcacctgca ggcctgcgcg atcccaggtc tgcgcgacgc cgaaccacag
     3541 cgatgcgcca agtccgtcgc gcacgagctg ctgcgccggc ggctcgccga cctcggcctc
     3601 gacgcgcccg aaatgccggt ccccgtcggc cacgacgagc ggcgtgacca ccagcgagac
     3661 cggctcgccc gcgcgcagca cctgcacctg cagcggatgc gcctggccgt ccgccgccga
     3721 gccgcggatg cgctgcagca gctcgttgcg gtccgcgatc ggatggccgt cgatggcggt
     3781 gacgaggtcg cccttcctca ggccggcgcg cgccgcgggg ccgtccgcct gcacgcgccc
     3841 gagcaccggc cgcacgtaca cgccgaggcc gaggcgcgcg aacacgtcgg cgtcgatctc
     3901 ggtcttgcca agcgaaggca acggcagcgt cagggtgcgc tcgcccgcgc cgtcgggcgc
     3961 gcgcacgcgc agcagcagcg ggcgctggtc catcagcgcc acggcgacgt tctcgtagat
     4021 gtcctcgtcg gaac
//
LOCUS       DASVLN010000871         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_206389, whole genome shotgun sequence.
ACCESSION   DASVLN010000871 DASVLN010000000
VERSION     DASVLN010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206389"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF453_20490"
     CDS             complement(<1..203)
                     /locus_tag="VF453_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690097.1"
                     /translation="MSQAAFNFASGKRAVKSTARQARAAAPVAARAEGSDPGDAIGFD
                     YARYGLVPPAPCLWPGHPVREGW"
     gene            294..1325
                     /locus_tag="VF453_20495"
     CDS             294..1325
                     /locus_tag="VF453_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX7690098.1"
                     /translation="MDRTERFYRIELLIKRQSGVSFEQLLAELEVSPATLKRDLQYLR
                     DRMDAPIVYARDENVYRFADGAGVTTQVAGRTGLVKQHELPGVWFSEKELHALLTMHQ
                     LIAGLDDGGVLGRHLQPLLDKLHGMLGASGDEAQALMRRVRIANPARRPVPSRFFEEA
                     VSAVLSRRRLAITYYTRGKRAESERVLSPQRLLHYRNTWYLDAWCHASEGLRRFALDA
                     VRAAKVLDEPARDLPLSKVESELDQGYGIFGGARLRWATLHFTAEAAQWVAQEEWHPQ
                     QQLKAQADGSLRMRLPYSDPTELVMDVLRHGPNVRVVAPAELAQQVRSQLERTLAAYG
                     RVEAGVEAD"
     gene            1337..1912
                     /gene="greB"
                     /locus_tag="VF453_20500"
     CDS             1337..1912
                     /gene="greB"
                     /locus_tag="VF453_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX7690099.1"
                     /translation="MNKAFTRESDAADDDDDDAGLPPLPAGGKNYITPQGQARLRAEL
                     LGLIDDERPKVVEVVHWAASNGDRSENGDYIYGKKRLREIDRRIRFLIKRLDIAVVAD
                     PSAHFGSDQVFFGATVTYANEAGDERTVTIKGIDEVDPLQGEVSWISPIARTLLKSRE
                     GDTLQLVTPGGVETIEVVAVRYPAPGTAAPH"
     gene            1920..2738
                     /locus_tag="VF453_20505"
     CDS             1920..2738
                     /locus_tag="VF453_20505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEX7690100.1"
                     /translation="MNATSPSTIRSLLAALALAASAGVAPAASASPKPASIPVYGYRV
                     VHAYPHDLNAFTEGLFYLDGYLYESTGLEGRSSVRKVKLETGQVVDRASLPPDIFGEG
                     IAAWGDKLVGLTWKNQVGYVLKLDGFDTLGEFHYPGEGWGLTHDDTELVMSDGTSDIR
                     FLDPSTLRETHRIHVTADGRPVDQINELEWVEGEIYANVWQTDRIARIDPKTGHVVGW
                     IDLAGLLPKADYIPNHTDVLNGIAYDPAGKRLFVTGKMWPKLFEIALVRKAAAR"
     gene            complement(2747..>3122)
                     /locus_tag="VF453_20510"
     CDS             complement(2747..>3122)
                     /locus_tag="VF453_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690101.1"
                     /translation="VALAGLRPWVEPFTHRPARAGRMDLDLDIRVAHRMLHTPGHLVV
                     TGLAFDDRPAPLAGLGHRALLAALTRDDRLDLRFTLQGRTDDPAFSLDEAIGRRAAAG
                     LGAAVGSGIRDVIGHLAGVRPG"
BASE COUNT          492 a         1104 c         1122 g          404 t
ORIGIN      
        1 tcccagccct cgcgcaccgg gtggcccggc cacaggcagg gcgcgggcgg cacgaggccg
       61 tagcgcgcat agtcgaagcc gatcgcgtcg cccggatcgc tgccctcggc gcgcgccgcg
      121 acgggggcgg cggcgcgggc ctgccgggcg gtggacttga cggcgcgctt gccggaggcg
      181 aagttgaagg cggcctggct catgatgcgt cctcggttcg tctggagtgt gaagccagtg
      241 tggcgctcca caagctcata cgatgagcca ggtacggcaa aatacttgcc accatggatc
      301 gcaccgaacg cttctatcgc atcgagctgc tcatcaagcg ccagtccggc gtcagcttcg
      361 agcagctgct ggccgagctc gaggtctcgc ccgccacgct caagcgcgac ctgcagtacc
      421 tgcgcgaccg catggacgcc cccatcgtct acgcgcgcga cgagaacgtc taccggttcg
      481 cggacggcgc cggcgtcacc acgcaggtgg cgggccgcac gggtctcgtg aagcagcacg
      541 agctgcccgg cgtctggttc agcgagaagg agctgcacgc cctgctgacg atgcaccagc
      601 tcatcgccgg cctggacgac ggcggcgtgc tcgggcgcca cctgcagccg ctgctggaca
      661 agctgcacgg catgctgggc gcgagcggcg acgaggcgca ggcgctcatg cgccgcgtgc
      721 gcatcgccaa cccggcgcgg cgcccggtgc ccagccgctt cttcgaggag gccgtgagcg
      781 cggtgctgag ccgccgccgc ctcgcgatca cctactacac gcgcggcaag cgggcggagt
      841 ccgagcgcgt gctgtcgccg cagcggctgc tccactaccg caacacctgg tacctcgacg
      901 cctggtgtca cgcgagcgaa ggcctgcgcc gcttcgccct cgacgccgtg cgcgcggcca
      961 aggtgctcga cgagccggca cgcgacctgc cgttgtccaa ggtggagtcg gagctggacc
     1021 agggctacgg catcttcggc ggcgcgcggc tgcgctgggc gacgctgcac ttcacggccg
     1081 aggccgcgca gtgggtcgcg caggaggaat ggcacccgca gcagcagctg aaggcgcagg
     1141 cggacggctc gctgcgcatg cggctgccgt actccgatcc gacggagctc gtgatggacg
     1201 tgctgcgcca cggccccaac gtgcgcgtcg tcgcgccggc ggagctcgcg cagcaggtgc
     1261 gctcgcagct ggagcgaacg ctcgcggcct acgggcgcgt cgaggcgggc gtcgaggcgg
     1321 actgaggagt cgcgagatga acaaggcctt caccagggaa tccgacgcgg cggacgacga
     1381 cgatgacgac gccggcctgc cgccgctgcc cgcggggggc aagaactaca tcacgccgca
     1441 gggccaggcg cgcctgcgcg ccgagctgct cggactgatc gacgacgaac ggcccaaggt
     1501 ggtcgaggtc gtgcactggg ccgcctccaa cggcgaccgc agcgagaacg gcgactacat
     1561 ctacgggaag aagcgcctgc gcgagatcga ccgccgcatc cgcttcctca tcaagcggct
     1621 ggacatcgcg gtcgtcgccg atccgtcggc ccatttcggc agcgaccagg tcttcttcgg
     1681 cgccaccgtc acctatgcca acgaggcggg cgacgaacgc accgtcacga tcaagggcat
     1741 cgacgaggtc gacccgcttc agggcgaggt cagctggatc tcgccgatcg cgcgcaccct
     1801 gctcaaatcg cgcgagggcg acacgctgca gctggtgacg cccggcggcg tcgagacgat
     1861 cgaggtcgtc gccgtgcgct atccagcgcc cggaaccgcc gcgccgcatt gaaggaacca
     1921 tgaacgccac gagcccgtcc acgatccgat ccctcctcgc cgcgctggcg ctcgccgcgt
     1981 ccgcgggcgt cgcgccggcc gcatccgcgt cgccgaagcc ggcctcgatc ccggtctacg
     2041 gctaccgcgt cgtccacgcc tatccgcacg acctcaacgc cttcacggaa ggcctgttct
     2101 acctggacgg ctacctgtac gagagcacgg gcctcgaggg gcgctcgtcg gtgcgcaagg
     2161 tcaagctcga gaccggccag gtcgtcgacc gcgccagcct gcccccggac atcttcggcg
     2221 agggcatcgc cgcctggggc gacaagctgg tcggcctgac ctggaagaac caggtcggct
     2281 acgtgctcaa gctcgatggc ttcgacaccc tcggcgagtt ccactatccc ggcgagggat
     2341 ggggcctgac gcacgacgac acggagctcg tgatgagcga cggcacgtcc gacatccgct
     2401 tcctcgaccc gtcgacgctg cgcgagacgc accgcatcca cgtcaccgcg gacggcaggc
     2461 cggtcgacca gatcaacgag ctggagtggg tcgagggcga gatctacgcc aacgtctggc
     2521 agaccgaccg catcgcccgc atcgacccga agacgggtca cgtcgtcggc tggatcgacc
     2581 tggccggcct gctgccgaag gccgactaca tcccgaacca caccgacgtc ctcaacggca
     2641 tcgcctacga cccggcaggc aagcgcctgt tcgtcaccgg caagatgtgg ccgaagctgt
     2701 tcgagatcgc gctcgtgagg aaggccgcgg cgcgctgacg gggacgtcag ccgggccgga
     2761 cgccggccag gtggccgatg acgtcccgga tcccgctgcc caccgcggcg cccaggcccg
     2821 cggctgcgcg tcggccgatg gcctcgtcga gcgagaaggc ggggtcgtcg gtgcggccct
     2881 gcagggtgaa gcgcaggtcg aggcggtcgt cgcgggtcag cgcggcgagc agcgcgcgat
     2941 ggccgaggcc ggccagcggg gccgggcggt cgtcgaaggc gaggccggtg acgaccaggt
     3001 ggcccggggt gtgcagcatg cggtgggcga cgcggatgtc gaggtcgagg tccatgcgtc
     3061 cggcgcgcgc gggccggtgc gtgaagggct cgacccaggg ccgcaggccg gcgagcgcga
     3121 cg
//
LOCUS       DASVLN010000872         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_367900, whole genome shotgun sequence.
ACCESSION   DASVLN010000872 DASVLN010000000
VERSION     DASVLN010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367900"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VF453_20515"
     CDS             <1..634
                     /locus_tag="VF453_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SUV3 C-terminal domain-containing protein"
                     /protein_id="HEX7690102.1"
                     /translation="YGLHEEGFAGVLREAEPGAARALKELLPRPPRAPRDFKAAVAPN
                     WWHVETIASRLHLTSLRAVLDVFVDQLKLDDAHFAVAELDQMLALAAELDRNAATLSL
                     RQRFIYAQAPVDTRTETLVQAFLEWTWHHATSGSVPAPWFLSEVDQHSRLDTMEKALR
                     ACTLWLWLDLRFPGIYGSVEEVLDLRSRLNDGIERHLKGKKPLWQRGGRR"
     gene            722..1024
                     /locus_tag="VF453_20520"
     CDS             722..1024
                     /locus_tag="VF453_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7690103.1"
                     /translation="MATLAKRLKEARLRAGLTQARLGVLAGIDEASASARMNQYERGK
                     HEPDWLTVQRLAQALAVPVAYLYAEDDATAELLLLFHALPLEAREEAVADFRKRLE"
     gene            complement(1106..>1827)
                     /locus_tag="VF453_20525"
     CDS             complement(1106..>1827)
                     /locus_tag="VF453_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7690104.1"
                     /translation="VLDGATGIEREFGIGLPEGEPPGAAPLRVGASTTIGNYLLPALI
                     ASWRRAWPGAQVDVRIANTGDIAAAVARLEVDVGFIEGPCHEPGVHAQPWREETLAIV
                     CAPGHPLLQGDPGRRLGVGALRQADWLLREPGSGTREAVEHALLPHLHQLREGLRFGG
                     TEAIKQAAVEGLGVTCLSHFAVRDLLALGRLVEVRTTLPRLVRRFYLVHHARKQFSRT
                     LRRFVEHCAQDAAGPAQHAIR"
BASE COUNT          265 a          668 c          670 g          224 t
ORIGIN      
        1 ctacggcctg cacgaggaag gcttcgcggg cgtgctgcgc gaggccgagc ccggcgccgc
       61 gcgcgcgctc aaggagctgc tgccgcgccc gccgcgcgcg ccgcgcgact tcaaggcggc
      121 cgtggcgccc aattggtggc atgtcgagac catcgccagc cggctgcacc tgaccagcct
      181 gcgcgcggtg ctggacgtct tcgtcgacca gctcaagctc gacgacgcgc acttcgcggt
      241 cgccgagctc gaccagatgc tggcgctcgc cgcggagctc gatcgcaacg ccgccacgct
      301 gtcgctgcgg cagcgcttca tctatgccca ggcgccggtg gacacccgca ccgagaccct
      361 cgtgcaggcc ttcctcgaat ggacctggca ccacgccacc agcggcagcg tgccggcgcc
      421 gtggttcctg tccgaggtcg accagcacag ccgcctggac acgatggaga aggcgctgcg
      481 cgcttgcacg ctgtggctgt ggctcgacct gcgctttccc ggcatctacg gcagcgtcga
      541 ggaggtgctg gacctgcgca gccggctcaa cgacggcatc gagcgccacc tgaaaggcaa
      601 gaagccgctg tggcagcgcg gcgggaggcg ctgacgcacg cccgcgacgc ctaagcattc
      661 cttagcacat cgcccagtca aagtacagac catcgcgccc tgacatgttc cgggacgatc
      721 gatggcgacg ctggcgaagc gactgaagga ggcacggctg cgcgccggcc tcacgcaggc
      781 gcggctgggc gtgctggccg ggatcgacga ggcgtccgcc agcgcgcgca tgaaccagta
      841 cgagcgcggc aagcacgagc cggactggct caccgtgcag cgactcgcgc aggcgctcgc
      901 ggtgccggtg gcctacctct acgccgagga cgacgcgacc gcggagctgc tgctgctgtt
      961 ccacgcgctg ccgctggagg cgcgcgagga ggccgtcgcg gacttccgca agcggctcga
     1021 gtgagacggg gctccggccg gcagccggaa ggcgtcgccc ggcttcgggt gctgctcgcg
     1081 gcgcggtaag gccggatccc ggacgctacc ggatcgcatg ctgcgccggg ccggccgcgt
     1141 cctgcgcgca atgctcgacg aagcgccgca gcgtgcgcga gaactgcttg cgcgcgtggt
     1201 gcacgaggta gaagcggcgc acgaggcgcg gcagggtggt gcggacctcg acgaggcggc
     1261 cgagcgccag caggtcgcgc accgcgaaat gcgacaggca ggtgacgccg aggccctcga
     1321 ccgcggcctg cttgatcgcc tccgtgccgc cgaaccgcag gccctcgcga agctggtgca
     1381 ggtgcggcag cagcgcgtgc tcgacggcct cgcgcgtgcc cgaacccggc tcgcgcagca
     1441 gccagtcggc ctggcgcagc gcgccgacgc ccaggcgccg ccccgggtcg ccctgcagta
     1501 gcggatggcc gggcgcgcag acgatggcca gcgtctcctc gcgccagggc tgcgcatgca
     1561 cgccgggctc gtggcagggg ccctcgatga agccgacatc gacctccagc cgcgcgaccg
     1621 cggccgcgat gtcgccggtg ttggcgatgc gcacgtcgac ctgcgcgccc ggccaggcgc
     1681 ggcgccagga cgcgatgagc gcaggcagca ggtagttgcc gatcgtcgtg ctcgcgccca
     1741 cgcgcagcgg cgccgcgccg ggcggctcgc cctcgggcag cccgatgccg aactcgcgct
     1801 cgatgccggt cgcgccatcg agcaccg
//
LOCUS       DASVLN010000873         2905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_156790, whole genome shotgun sequence.
ACCESSION   DASVLN010000873 DASVLN010000000
VERSION     DASVLN010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156790"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1561)
                     /locus_tag="VF453_20530"
     CDS             complement(140..1561)
                     /locus_tag="VF453_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7690105.1"
                     /translation="MALFRPSFRQTLLAAFLLIAGLLAAASLQGLFTLERVMARSRAA
                     TERALVLTNDSRQLVDLRVAMERSARQYIVLDDPSLLRGFAGASEEATRLLAEMRRND
                     LPPALAQEWLAQLPAIKDQLGGTKASAQARSDALGPAFRTLDRLNAEIAEVVRTSDEQ
                     RNERLQAELEDGRATVGQQVLGAIVLAALLAIAFGVWLTRPLKRLERAILAMGANRMD
                     QRVVIRGPSDLRTIGQRLEWLRLRLKELDEDKARFLRYTSHELKTPLAALREGVALLE
                     DGVAGPMTADQREIARILGHNTAVLQGQIEALLRFNAAAFEARRLDRRRTDLRALLHA
                     LIEEQRLQWQARQLTIELDGEAEPLAVDAEKLSAALGNLLSNSIRFSPVGATIFIRLA
                     SQRGLVRIDLQDQGPGVAAADRARIFEPFFRGEVQPEGAVRGTGIGLAIVREYVEAHG
                     GRVELLPDGPGAHFRIELPHVND"
     gene            1686..2846
                     /locus_tag="VF453_20535"
     CDS             1686..2846
                     /locus_tag="VF453_20535"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7690106.1"
                     /translation="MEALKGLKVLELGQLIAGPFAGKTLAEFGADVIKVEPAAPGDPL
                     RQWRLLREGTSVWWEVQSRNKRSVCLDLRTPEGQSVARALAAEADVLIENFKPGTLEG
                     WGLGWEALHALNPKLIMLRISGYGQTGPYRDKPGFGVIGEAMGGLRHLTGEPGRVPVR
                     AGISIGDTLAALHGVIGVLVALHHRVAHGGEGQVVDVALYEAVFNVMESLVPEHDAFG
                     VVRERAGSALPGIAPTNAYPCNDGQYVVIGGNGDSIFRRLMHAIGRPDLADDPALKHN
                     DGRAKRSGELDAAIAAWTSVRSRDEALAVLDEARVPAGKIYSVADIAADPQYAARGMI
                     VDTPTSNGLPLKVPGIVPKLSATPGGIAHAAPRLGQHDADLARGEGWPARRD"
BASE COUNT          391 a         1025 c         1127 g          362 t
ORIGIN      
        1 cggcggcggt ggcggcggcg gcggcggtgg cggcaacggc gtcgactggc acccggccag
       61 gatcgcggcg agcgcgaaca gggcggccag cgacgacgcg cgtcccggcg ggtgcggcgt
      121 cgggcacgag gaggaagggt cagtcgttga catgggggag ctcgattcgg aaatgggcgc
      181 cgggcccgtc gggcagcagc tcgacgcggc cgccgtgcgc ctcgacgtac tcgcgcacga
      241 tcgccaggcc gatgccggtg ccgcggacgg cgccttcggg ctgcacctcg ccgcggaaga
      301 acggctcgaa gatgcgggcc cggtcggccg ccgcgacgcc cgggccctgg tcctggagat
      361 cgatgcgcac cagcccgcgc tgcgaggcga ggcggatgaa gatcgtggcg ccgacggggc
      421 tgaagcgtat cgaattggac aacaagttgc ccagcgccgc cgaaagcttc tccgcgtcga
      481 ccgccagcgg ctcggcctcg ccgtcgagct cgatggtcag ctggcgggcc tgccactgca
      541 ggcgctgctc ctcgatgagg gcgtgcagca gcgcgcgcag gtcggtgcgg cgacggtcga
      601 gccgtcgggc ctcgaaggcg gcggcgttga agcgcagcag cgcctcgatc tggccttgca
      661 gcaccgcggt gttgtggccg aggatgcgcg cgatctcgcg ctggtcggcg gtcatcgggc
      721 cggcgacgcc gtcctccagc agcgccacgc cctcgcgcag ggcggccagc ggcgtcttca
      781 gctcgtgcga ggtgtagcgc aggaagcgcg ccttgtcctc gtcgagctcc ttgagccgca
      841 gccgcagcca ctcgagccgc tggccgatgg tgcgcaggtc gctcgggccg cggatgacga
      901 cgcgctggtc catgcggttg gcgcccatcg cgaggatcgc gcgctccagc cgcttgagcg
      961 gccgcgtgag ccagacgccg aacgcgatcg ccagcagcgc ggcgagcacg atcgcgccga
     1021 gcacctgctg gcccacggtg gcgcggccgt cctcgagctc cgcctgcagg cgctcgttgc
     1081 gctgctcgtc gctcgtgcgc acgacctcgg cgatctcggc gttgaggcgg tcgagcgtgc
     1141 ggaacgccgg cccgagcgca tcggagcgcg cctgcgccga ggccttggtg ccgcccagct
     1201 ggtccttgat cgcgggcagc tgcgcgagcc attcctgcgc gagcgcgggc ggcaggtcgt
     1261 tgcggcgcat ctcggccagc aggcgcgtgg cctcctccga ggcgcccgcg aagccgcgca
     1321 gcagcgacgg gtcgtccagc acgatgtact gccgcgcgct gcgctccatc gcgacgcgca
     1381 ggtcgacgag ctggcgcgag tcgttcgtca gcaccagggc gcgttcggtg gccgcgcggc
     1441 tgcgcgccat cacgcgctcg agcgtgaaca gcccctgcag cgacgcggcc gccagcagcc
     1501 ccgcgatcag caggaaggcg gcgagcagcg tctggcgaaa cgagggtcgg aacagggcca
     1561 tgggcgaggg cggggcgcgg cggcgcgggc gccattgtca cttgtcgtcg gacgaagggt
     1621 gcgcggccgg gcgcggcgta cagttccggc gcccggtgga cgggccttgc ggggacgatg
     1681 cgaccatgga agcgctcaag gggttgaagg tgctggagct cgggcagctg atcgcgggcc
     1741 cgttcgccgg caagacgctc gccgagttcg gcgccgacgt catcaaggtc gagccggccg
     1801 cacccggcga tccgctgcgc cagtggcgcc tgctgcgcga gggcacgtcg gtgtggtggg
     1861 aggtgcagtc gcgcaacaag cgctcggtgt gcctggacct gcgcacgccc gagggccagt
     1921 ccgtcgcccg cgcgctcgcg gccgaggccg acgtgctgat cgagaacttc aagcccggca
     1981 cgctggaggg ctggggcctc ggctgggaag cgctgcacgc gctcaacccg aagctgatca
     2041 tgctgcgcat ctcgggctac ggccagaccg gcccctaccg cgacaagccg ggcttcggcg
     2101 tgatcggcga ggcgatgggc ggcctgcgcc acctcaccgg ggagcccggg cgcgtgccgg
     2161 tgcgcgccgg catctcgatc ggcgacacgc tggccgcgct gcacggggtg atcggcgtgc
     2221 tcgtcgcgct gcaccaccgc gtcgcgcatg gcggcgaggg ccaggtcgtc gacgtcgcgc
     2281 tgtacgaggc cgtcttcaac gtgatggaga gcctggtgcc cgagcacgac gcgttcggcg
     2341 tggtgcgcga gcgcgccggc agcgcgctgc ccggcatcgc cccgaccaac gcctatccgt
     2401 gcaacgacgg acagtacgtc gtgatcggcg gcaacggcga cagcatcttc cgccgcctga
     2461 tgcacgcgat cggccgaccc gacctcgccg acgacccggc gctgaagcac aacgacggcc
     2521 gcgcgaagcg cagcggcgag ctcgacgccg cgatcgccgc gtggacgtcg gtgcgctcgc
     2581 gcgacgaggc cctcgcggtg ctcgacgagg cgcgcgtgcc ggcgggcaag atctactcgg
     2641 tggccgacat cgcggccgac ccgcagtacg cggcgcgcgg gatgatcgtc gacacgccga
     2701 cctccaacgg cctgccgctg aaggtgcccg gcatcgtccc caagctgagc gcgacgcccg
     2761 gcggcatcgc ccacgccgcg ccgcggctcg ggcagcacga cgccgacctc gcgcgcggcg
     2821 agggctggcc cgcgcggcgc gactgaggat cggcgacgag gttcgcatcg tgcacccgcg
     2881 ccggggcgcg gcgcatgggc atgcg
//
LOCUS       DASVLN010000874         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_421654, whole genome shotgun sequence.
ACCESSION   DASVLN010000874 DASVLN010000000
VERSION     DASVLN010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421654"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..626)
                     /locus_tag="VF453_20540"
     CDS             complement(84..626)
                     /locus_tag="VF453_20540"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX7690107.1"
                     /translation="MSAAPASSWGLMAEYATAEALVAAAQAAHDAGYAHAEAYAPYAI
                     EGLPEALGFRRTRMPLVTFCGGLVGGIGGYFLQWYSAVVSYPENLGARPLHSWPMFVP
                     VTFELAVLGAAFAAFFGVLAANRLPRLAHPVFDAPDFELASRNRFFLVLRRDDPAWDE
                     PGAHGLLDATQALRRAEVPA"
     gene            complement(623..1975)
                     /gene="nrfD"
                     /locus_tag="VF453_20545"
     CDS             complement(623..1975)
                     /gene="nrfD"
                     /locus_tag="VF453_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX7690108.1"
                     /translation="MTTPPVSHDSDVLRAGWGYASVTDKIAGLVLERPLGRRWLLAFC
                     ATLAGTLIYLAAMAWLFYKGVGIWGVDIPVAWGFAIANFVWWIGIGHAGTFISAILLL
                     LRQKWRTSINRFAEAMTLFAATMAGLYPILHLGRPWFAYWLAPYPDKMNLWPQWRSPL
                     VWDVFAISTYLVVSALFWYLGMLPDLATMRDRARTRFKRVAYGLAALGWRGEARHWAR
                     HESAYLLMAGIATPLVVSVHSVVAMDFAIGNTPGYHSTIFPPYFVAGALFSGFAMVLT
                     LAIPLRRAFKLQDFITETHLDHAAKVLLATGAFVGYGYACEIFTAFWSGDRYEVAMTV
                     DRFTGVYAPVYWFMMACNVLAPQVLWWRRARRSTALLFALSLVVNLGMWMERVTIVVQ
                     STHHDFMPSAWGVFVPTRWDWLFLLGSISMFAWLFLVFIRLLPAISISEMRELVKEES
                     "
     gene            complement(1972..>2989)
                     /locus_tag="VF453_20550"
     CDS             complement(1972..>2989)
                     /locus_tag="VF453_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7690109.1"
                     /translation="EAPVRIVAAQAEEVVTVPLGLGRRRAGRVGDRVGFDGYALRPGA
                     ARIAPIELHPLGRRHDFALTQHETVQHERELARTLAPGEAMPPEPPRASVYPPFASPV
                     HAWAMTIDLDACIGCNACTVACQAENNIPVVGRDQVALGRVMHWIRVDRYDDAPPDGD
                     AARSIFQPVPCMHCEEAPCEVVCPVGAAVHDSEGLNLQVYNRCVGTRFCSNNCPYKVR
                     RFNFLQYSDNDTETLKAQRNPEVTVRRRGVMEKCTYCLQRVARARQRSEREGVPIADG
                     DVATACQTACPTRAIRFGDLDDPRSEVVRSKASPRHYAMLGELGTRPRTTYLARVGPP
                     KDES"
BASE COUNT          481 a         1025 c         1105 g          378 t
ORIGIN      
        1 ctgcgcgtac atctcgcgca ggtcgcgctc gcagccgcac aggacgagcg cggccaggcc
       61 cagcgcgagc agacccgggc gcctcatgcc ggcacctccg cgcgccgcag cgcctgcgtg
      121 gcgtcgagca ggccgtgggc gccgggctcg tcccaggccg ggtcgtcgcg ccgcagcacg
      181 aggaagaagc ggttgcgcga cgcgagctcg aagtcgggcg cgtcgaagac cggatgcgcg
      241 agccgcggca ggcggttggc ggcgagcacg ccgaagaagg cggcgaatgc cgcgccgagc
      301 accgcgagct cgaaggtcac gggcacgaac atcggccagc tgtgcagcgg gcgtgcgccg
      361 aggttctcgg gatagctcac gaccgccgag taccactgca ggaagtaccc cccgatgccg
      421 ccgacgagtc cgccgcagaa cgtgaccagc ggcatgcgcg tgcgccggaa gcccagcgcc
      481 tcgggcaggc cctcgatcgc gtagggcgcg taggcttcgg cgtgcgcgta gccggcgtcg
      541 tgcgcggcct gcgcggcggc gacgagcgcc tcggcggtcg cgtactccgc catcagcccc
      601 caggacgacg cgggcgcagc gctcatgact cctccttcac gagctcgcgc atctccgaga
      661 tcgagatcgc aggcagcagc cggatgaaca cgaggaacag ccacgcgaac atgctgatcg
      721 agcccagcag gaacagccag tcccagcgcg tcgggacgaa cacgccccac gccgagggca
      781 tgaaatcgtg gtgggtgctc tggacgacga tcgtcacgcg ctccatccac atgcccaggt
      841 tgacgacgag cgacagcgcg aacagcagcg ccgtgctgcg ccgcgcgcgc cgccaccaca
      901 gcacctgcgg ggcgaggacg ttgcaggcca tcatgaacca gtagaccggc gcgtacacgc
      961 cggtgaagcg gtcgaccgtc atcgccacct cgtagcggtc gccgctccag aacgccgtga
     1021 agatctcgca ggcgtagccg tagccgacga aggcgccggt ggccagcagc accttggcgg
     1081 cgtggtcgag gtgcgtctcg gtgatgaagt cctgcagctt gaacgcgcgc cgcagcggga
     1141 tcgcgagggt gagcaccatc gcgaagcccg agaacagcgc gccggcgacg aagtagggcg
     1201 ggaagatggt cgagtggtag ccgggggtgt tgccgatcgc gaagtccatc gcgacgaccg
     1261 agtgcaccga taccaccagg ggtgtcgcga tgcccgccat cagcaggtag gccgattcgt
     1321 ggcgcgccca gtgccgcgcc tccccgcgcc agcccagcgc agcgagcccg taggcgaccc
     1381 gcttgaagcg ggtgcgcgcg cggtcgcgca tcgtcgcgag gtcgggcagc atgcccaggt
     1441 accagaacag ggcggagacg acgaggtagg tcgagatcgc gaacacgtcc cacacgagcg
     1501 ggctgcgcca ctgcggccac aggttcatct tgtcggggta gggcgcgagc cagtacgcga
     1561 accagggtcg gcccaggtgc aggatcgggt agagcccggc catcgtggcc gcgaacagcg
     1621 tcatcgcctc ggcgaagcgg ttgatggacg tgcgccactt ctgccgcagc aacaaaagga
     1681 tcgccgagat gaacgtgccg gcgtgcccga tgccgatcca ccagacgaag ttggcgatcg
     1741 cgaagcccca ggcgaccggg atgtcgacgc cccagatgcc gacgcccttg tagaacagcc
     1801 acgccatcgc cgccaggtag atgagcgtgc cggccagcgt cgcgcagaac gcgagcagcc
     1861 agcggcggcc gagcggacgc tcgagcacca gccccgcgat cttgtcggtg acgctcgcgt
     1921 agccccagcc ggcgcgcagg acgtcgctgt cgtgggagac gggaggcgtc gtcacgattc
     1981 gtccttgggc gggcccacgc gcgccaggta ggtcgtgcgc ggccgcgtgc cgagctcgcc
     2041 cagcatcgcg tagtggcgcg gggaggcctt gctgcgcacg acctcgctgc gcgggtcgtc
     2101 gaggtcgccg aagcggatcg cgcgcgtcgg gcaggcggtc tggcaggcgg ttgcgacgtc
     2161 gccgtcggcg atgggcacgc cctcgcgctc gctgcgctgg cgcgcgcggg cgacgcgctg
     2221 caggcagtag gtgcacttct ccatcacgcc gcgccgtcgc acggtgacct cggggttgcg
     2281 ctgagccttc agcgtctcgg tgtcgttgtc cgagtactgc aggaagttga accgccgcac
     2341 cttgtacggg cagttgttgg agcagaagcg cgtgccgacg cagcggttgt agacctggag
     2401 gttcagcccc tcgctgtcgt gcacggcggc gccgaccgga cagacgacct cgcacggcgc
     2461 ctcttcgcaa tgcatgcacg gcacgggttg gaagatgctc cgggccgcat cgccgtcggg
     2521 cggcgcgtcg tcgtagcggt ccacgcggat ccagtgcatc acgcggccga gcgcgacctg
     2581 gtcgcggccg acgaccggga tgttgttctc ggcctggcac gcgacggtgc aggcgttgca
     2641 gccgatgcag gcgtcgaggt cgatcgtcat cgcccacgcg tgaacggggc tcgcgaacgg
     2701 cggatagacg cttgcgcgcg gcggctccgg cggcatcgcc tcgccgggcg ccagcgtgcg
     2761 cgcgagctca cgctcgtgct gcacggtctc gtgctgggtg agggcgaagt cgtggcggcg
     2821 gcccagcggg tgcagctcga tgggggcgat ccgcgccgcg cccggacgca gcgcgtagcc
     2881 gtcgaagccg acgcggtcgc ccacgcgtcc ggcacgacga cggcccagcc ccagcggcac
     2941 ggtcacgacc tcctcggcct gggcggcgac gatgcgcacg ggcgcctcg
//
LOCUS       DASVLN010000875        10737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_43970, whole genome shotgun sequence.
ACCESSION   DASVLN010000875 DASVLN010000000
VERSION     DASVLN010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43970"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF453_20555"
     CDS             complement(<1..306)
                     /locus_tag="VF453_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690110.1"
                     /translation="MALYKGIEFPDQVAPFARHMPALNDHREALAALGATWDTLGLLA
                     HLSNLKADMREVRAGFGRLTAELLGSLAVETLARATAVLGRQARIAVDVLARDLAGRG
                     "
     gene            complement(361..1467)
                     /locus_tag="VF453_20560"
     CDS             complement(361..1467)
                     /locus_tag="VF453_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7690111.1"
                     /translation="MTATATVTVTAAARADVAVLVGRFQPCHAAHLALLRHALAAAPR
                     CIVVIGSAHQARSPKNPFTWTERAEMLRLALPEGERERVRFLPVRDYYDEARWVAAVR
                     AGVDAELFAQSLPPTASVVLVGHFKDATSGYLRAFPGWRLESVPRLGDAGGTHLRDAL
                     FASVPEDAHAPDALEPALASLVDQVPPSTIAFLRAWARLPFLAELAAEWRMLRAYREA
                     WRAAPYPPVFVTVDAVVRCDGHVLLIRRGKAPGRGLLAVPGGFIELRETCWQSCLREL
                     EEETHLVLLEESWKAALREVAVFDHPDRSQRGRTITHAHYFDLGERDLPEARADDDAA
                     AVEWVPIGELPALEDQFHDDHFHMLDHFLGLTTG"
     gene            complement(1481..3181)
                     /locus_tag="VF453_20565"
     CDS             complement(1481..3181)
                     /locus_tag="VF453_20565"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX7690112.1"
                     /translation="MLKITIAQLNYTVGDIAGNVARMAAAAREAAAAGSGLVVFSELS
                     LTGYYPGDLLDEPEFLVRVAHGLEALRAASCETPGLAWIVGAPMPREGVGKRLENALL
                     VLRDGEVVLRYAKQLLPTYNIFDESRHFAPGPDVARVLRVGDSRVGLMICEDGWNDVG
                     ADYAVNPFLRLADAQPDLVVSINASPSEIGKRETRHQIFGDACRRHGLPLVYVNQVGG
                     QDQLVFDGASFAVDAGGAVTFEARRFVEDVTTLRFEDGRFAAADGTALAPVPPAGMPT
                     MAFYRAQIVLGLRDYARRCGFSKAVVGSSGGIDSALTIALAADALGADNVVAVTMPSV
                     YSSAGSVDDSVALCRNLGVRLHTHPIAPMVDAFAAQFAQAFGEPLKGLPLENLQARVR
                     GTTLMEYSNTYGHLLLTTGNKSEISVGYCTLYGDTNGGLGLIGDLYKTEVFELSRHVN
                     EAAGRELIPQAIIDKEPSAELAPGQRDVDSLPPYPVLDQILRVLIEGRRLAAREFDAA
                     RQFVDDLRATPEGEALVQRVRGMIARNEYKRRQAPPIVRVRPRAFGTGRQMPIAAKTF
                     "
     gene            complement(3204..4409)
                     /gene="pncB"
                     /locus_tag="VF453_20570"
     CDS             complement(3204..4409)
                     /gene="pncB"
                     /locus_tag="VF453_20570"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX7690113.1"
                     /translation="MIPVIHSLLETDLYKFTMWQTMLHRHPAAQAEYRFTCRNQPAFP
                     LALLVDEVNEQLDHLCSLRFTDDEVAYLGGLRYMKSDFVDYLRIFHFQRQFVQARAGA
                     DGRTLEIVARGPQVHVMGFEIYVLEIVNELYFRRVEQVPAIEEGRRRLRAKLALLREY
                     DAERGGREGCPFEFFDFGIRRRFSGAWHEEVVRTLQREAPEHFKGTSNVHFARTMGLV
                     AIGTMAHEYLQSYQAFDVRLRDFQKAAFENWVQEYRGDLGIALTDVVGMDAFLRDFDL
                     YFAKLFDGLRHDSGDPFDWGEKALAHYAKLRIDPRTKRLVYSDGLDLPAAFRLHRHFA
                     TRTQLGFGIGTNLSNDCGHVPLNIVMKLTECNGQPVAKLSDSPGKTLCDDETFLAYLR
                     QVFQIAQPA"
     gene            complement(4446..5318)
                     /locus_tag="VF453_20575"
     CDS             complement(4446..5318)
                     /locus_tag="VF453_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX7690114.1"
                     /translation="MTRNVQLLVIDPQNDFCDVPAAYRPLDPLTGAPVAPALPVAGGH
                     ADMLRTAALIRTAGPAIDEITVTLDSHHQVDVAHPTFWRRGDGGAVAPFTPITAAQVR
                     AGEFAPRDAAALPRTLAYLDALEARGRYVLMVWPVHCEIGTWGHDVHADVRAAYNAWE
                     RATLRGVRKVTKGENPWTEHYSAIQAEVPDADDPHTLLNTALLEALDRAQLLLVAGEA
                     SSHCVRATTEHLVANLPSGRPERVVLLTDCMSPVGGFEAQQGEFFAQMRALGVRLMTS
                     AEALALVEAIAATA"
     gene            5496..5960
                     /locus_tag="VF453_20580"
     CDS             5496..5960
                     /locus_tag="VF453_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7690115.1"
                     /translation="MNKPGTGPSSDRHADRLHVLAADLRVLLGRLRRRLREQTGGGEL
                     PPAQVDVVHQLEREGAATIAQLARAQGMRPQSMGANVAALEAAGYVAGAPDPADGRQT
                     LWSLTPHCIAVMKAGRAAREDWIARTVQDHFSAAEQAELARGIALLKRIADS"
     gene            5970..6533
                     /locus_tag="VF453_20585"
     CDS             5970..6533
                     /locus_tag="VF453_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011882377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7690116.1"
                     /translation="MPITMLDPRTALVVVDLQKGIASAQTVPHAAAEVVARAARLAEA
                     FRAAGLPVVLVNVDGMAPGRTDPAPPRPPFPPGWDELVPELDAQASDLRVTKRTWGAF
                     AHTTLEAQLRAHGVTQVVVCGIATSAGVESTARAAHELGFNVTLPTDAMADRSDEAHE
                     GSVARVFPRIGETGPTADVLALLARRE"
     gene            6547..7848
                     /locus_tag="VF453_20590"
     CDS             6547..7848
                     /locus_tag="VF453_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7690117.1"
                     /translation="MRSTFRSLRGYNYRAWAIGAFVSNVGTWMQRTAQDWIVLTALTA
                     HSATAVGIVMALQFGPTAVLLPFAGFAADHFDRRKLLFWTQGAMGALALGLGLLTVTG
                     VVTLWEVYAFALALGVATAFDAPARQTFVSELVGESDLSNAVALNSTSFNAARLIGPA
                     VAGTMIHVLGSGWVFLLNAASYVAVLGSLRVLRVEELHRGARAARARGSFAEGLRYVR
                     GRPDLVAVLAMLFLIGTFGLNFPIFLSTMSVMVFHAGAREYGLLTSTMAVGSVLGALL
                     SAGQERPRAALLLGGAAAFGIGCAIAALMPDYWLFGAVLVAVGISVQVFNTTANATMQ
                     LSTDPVMRGRVMALVMAIALGGTPIGAPLVGWVADTFGPRWSLGVGAASGLLAAVVGV
                     RYLVRHRRLSVRLESGRLRFRLAPSAIAAAAAAVEAEASRF"
     gene            7986..8249
                     /locus_tag="VF453_20595"
     CDS             7986..8249
                     /locus_tag="VF453_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEX7690118.1"
                     /translation="MSLHPSHPDIVKRLKRAEGHLRSIVAMLEEGRDCLEIAQQLQAV
                     ESAVGNAKRTLVHDHIDHCLEHAVEGASAREAIREFKAITKYL"
     gene            8249..9190
                     /locus_tag="VF453_20600"
     CDS             8249..9190
                     /locus_tag="VF453_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux transporter"
                     /protein_id="HEX7690119.1"
                     /translation="MNDLTSLLQHGGNAWLFLPTAIVLGALHGLEPGHSKTMMAAFII
                     AVRGTLGQAVLLGLAATASHTAVVWLVALGGLYFGQRWDAEASEPILQLVSAAIIVGV
                     GAWMVWRTSRNQAACFYDHGDAAHGHDHAHDHARDDRARDHRHDHARAHGPVAHVHGA
                     ACSHGDDHDHDHDHDHDHDHDPAPVATADDPHGHGADIQRRFAGRHVTTAQIVMFGLT
                     GGLIPCPASITVLLLCLQLKRLALGATLVLGFSIGLAATMVASGVVAAIGVKHLSKRA
                     DASGRLSALAAKAPYLSGGLILLVGAVLAWQALRAMG"
     gene            9348..9695
                     /locus_tag="VF453_20605"
     CDS             9348..9695
                     /locus_tag="VF453_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEX7690120.1"
                     /translation="MPHASHHPTKPKSPSPHRWSQHVAETSDALDLPKDMFKQSDPKR
                     IAADLKRSAEHSSRRHAEPYRSAMSMLTFYVNRAGKTLGATQKRRLEAAKDELRALFG
                     RPRRDASSSGARK"
     gene            9692..10075
                     /locus_tag="VF453_20610"
     CDS             9692..10075
                     /locus_tag="VF453_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEX7690121.1"
                     /translation="MKSPGHRQHPGHRVLERGMPGRLQACVGGDVLAESDDVLEVDED
                     GNPPRYYFPRGDVRAQALQRTASPTHCPYKGDGVLFAVRLGDGTTVADGAWSYEQPYD
                     EHAALKDRVAFWSEKFENIEFKAVP"
     gene            complement(10082..>10737)
                     /locus_tag="VF453_20615"
     CDS             complement(10082..>10737)
                     /locus_tag="VF453_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690122.1"
                     /translation="AVALAGGPKPLGPVDPSALATSQATLRLIPGQFVQVHADGLPDP
                     AAPSSDPFVRVLPLQVDPNAVKVGARWGSEDRIDAPLLSQIALSTQADLFAGPAPRTV
                     RRSLRISAQWDSLSDLTFGLTPGVQRIGGGTLEHYVAGLQASTLDPHAPPAHWRSFIE
                     VSGEKISPGNLIENSTAQVHAGATYASSKSTQLDFSVTRGTMYQSDLTSSVGLQVKF"
BASE COUNT         1462 a         3780 c         3984 g         1511 t
ORIGIN      
        1 gccgcggccg gcgaggtcgc gcgcaagcac gtccaccgcg atgcgcgcct ggcgcccgag
       61 cacggccgtc gcgcgcgcga gcgtctcgac cgcgaggctg cccagcagct cggccgtcag
      121 gcgcccgaag ccggcgcgca cctcgcgcat gtcggccttg agattggaca ggtgcgccag
      181 cagcccgagc gtgtcccagg tcgcgccgag cgcggccagc gcctcgcggt ggtcgttgag
      241 cgccggcatg tggcgcgcga acggcgccac ctggtcgggg aactcgatgc ctttgtagag
      301 cgccatcgcg gcgcatgtta gggcgcgccg cagggggcgt cgagtcgggt cgactaccgg
      361 tcagcccgtg gtcagcccga ggaagtggtc gagcatgtgg aagtggtcgt cgtggaactg
      421 gtcttccagt gccggcagct cgccgatggg cacccattcg accgcggcgg cgtcgtcgtc
      481 ggcgcgcgcc tcgggcaggt cgcgctcgcc gagatcgaag tagtgcgcat gcgtgatggt
      541 gcggccgcgc tggctgcggt ccgggtggtc gaacacggcg acctcgcgca gcgcggcctt
      601 ccaggactcc tcgagcagca cgaggtgcgt ctcctcctcg agctcgcgca ggcaggactg
      661 ccagcaggtc tcgcgcagct cgatgaagcc gcccggcacc gcgagcaggc cgcggcctgg
      721 cgccttgccg cggcggatca gcagcacgtg tccgtcgcag cgcacgacgg cgtcgaccgt
      781 cacgaagacc ggcgggtacg gcgccgcgcg ccaggcctcg cggtaggcgc gcagcatgcg
      841 ccactcggcc gcgagctcgg ccaggaaggg cagccgcgcc caggcgcgca ggaaggcgat
      901 cgtcgacggc gggacctggt cgacgaggct cgcgagcgcg ggctcgagcg cgtcgggggc
      961 gtgcgcgtcc tcgggcacgc tcgcgaacag ggcgtcgcgc aggtgcgtgc cgccggcgtc
     1021 gccgagcctg ggaacggact cgagccgcca gcccgggaag gcccgcaggt agccgctggt
     1081 cgcgtccttg aagtggccga ccagcaccac cgaggcggtc ggcggcagcg actgcgcgaa
     1141 gagctccgcg tcgacgcccg cgcgcaccgc ggcgacccag cgcgcctcgt cgtagtagtc
     1201 gcgcaccggc aggaagcgca cgcgctcgcg ctcgccctcg ggcagcgcca ggcgcagcat
     1261 ctcggcgcgc tccgtccagg tgaaggggtt cttgggcgag cgcgcctggt gcgccgagcc
     1321 gatcacgacg atgcagcgcg gcgccgcggc gagcgcgtgc cgcagcagcg cgaggtgggc
     1381 ggcgtggcag ggctggaagc ggccgacgag gaccgcgacg tcggcgcggg cagcggcggt
     1441 gacggtgacg gtggcggttg cggtcaaggg cgtgcctcct tcagaacgtc ttcgcggcga
     1501 tgggcatctg ccgcccggtg ccgaacgcgc gcggacgcac gcgcacgatc ggcggcgcct
     1561 ggcggcgctt gtactcgttg cgcgcgatca tgccgcgcac gcgctgcacc agggcctcgc
     1621 cctcgggcgt ggcgcgcagg tcgtcgacga actgccgcgc ggcgtcgaac tcgcgcgcgg
     1681 cgagccggcg gccctcgatc agcacgcgca ggatctggtc cagcacggga tagggcggca
     1741 ggctgtcgac gtcgcgctgg ccgggggcga gctcggcgct gggttccttg tcgatgatgg
     1801 cttgggggat cagctcgcgt cccgcggcct cgttgacgtg gcgcgagagc tcgaagacct
     1861 cggtcttgta gaggtcgccg atcagcccga ggcccccgtt ggtgtcgccg tacagcgtgc
     1921 agtagcccac cgagatctcc gacttgttgc cggtggtcag cagcagatgc ccgtacgtgt
     1981 tggagtattc catcagcgtg gtgccgcgca cgcgcgcctg gaggttctcc agcggcaggc
     2041 ccttcagcgg ctcgccgaag gcctgcgcga actgcgccgc gaaggcgtcg accattggcg
     2101 cgatcggatg cgtgtgcagc cggacgccga ggttgcggca cagcgcgacc gagtcgtcga
     2161 ccgagcccgc cgacgagtac acggagggca tggtcaccgc gacgacgttg tccgcgccga
     2221 gcgcgtcggc cgcaagcgcg atcgtcaggg cgctgtcgat gccgccggag gagccgacga
     2281 cggccttcga gaagccgcag cggcgggcgt agtcgcgcag gcccagcacg atctgcgcgc
     2341 ggtagaaggc catcgtcggc atgcccgcag ggggtaccgg cgccagcgcc gtgccgtcgg
     2401 cggcggcgaa gcggccgtcc tcgaagcgca gcgtcgtgac gtcctcgacg aagcgccggg
     2461 cctcgaaggt caccgcgccg ccggcgtcga ccgcgaacga ggcgccgtcg aacacgagct
     2521 ggtcctggcc gccgacctgg ttgacgtaga cgagcggcag cccgtggcgg cggcaggcgt
     2581 cgccgaagat ctggtggcgc gtctcgcgct tgccgatctc cgacgggctc gcgttgatcg
     2641 acacgacgag gtcgggctgc gcgtccgcga ggcgcaggaa cgggttgacc gcgtagtcgg
     2701 cgccgacgtc gttccagccg tcctcgcaga tcatcagccc gacgcgcgaa tcgcccacgc
     2761 gcaggacgcg cgcgacgtcg gggccgggcg cgaaatggcg cgactcgtcg aagatgttgt
     2821 aggtcggcag cagctgcttg gcgtagcgca gcacgacttc gccgtcgcgc agcacgagca
     2881 gcgcgttctc gaggcgcttg ccgacgccct cgcgcggcat cggcgcgccg acgatccagg
     2941 cgaggccggg cgtctcgcag gaggccgcgc gcaaggcctc gaggccgtgc gcgacgcgga
     3001 ccaggaactc gggctcgtcg agcaggtcgc ccgggtagta gcccgtcagc gagagctcgg
     3061 agaacaccac cagcccgctg ccggcggccg cggcctcgcg cgcggcggcg gccatgcggg
     3121 cgacgttgcc ggcgatgtcg ccgacggtgt agttcagttg cgcgatcgtg atcttcaaca
     3181 tggtgctgga ttcccgtcgg gtgtcaggcc ggctgcgcga tctggaagac ctggcgcagg
     3241 taggcgagga aggtctcgtc gtcgcacagc gtcttgccgg gcgagtccga cagcttggcc
     3301 accggctggc cattgcactc ggtgagcttc atgacgatgt tcagcggcac gtggccgcag
     3361 tcgttgctca ggttggtgcc gatgccgaag ccgagctgcg tgcgggtcgc gaaatggcgg
     3421 tgcaggcgga atgccgccgg caggtcgagg ccgtcggaat agacgagccg cttcgtgcgc
     3481 gggtcgatgc gcagcttcgc gtagtgcgcg agcgccttct cgccccagtc gaacgggtcg
     3541 cccgagtcgt ggcgcaggcc gtcgaagagc ttggcgaagt agaggtcgaa gtcgcgcagg
     3601 aaggcgtcca tgccgaccac gtcggtcagc gcgatgccga ggtcgccgcg gtactcctgc
     3661 acccagttct cgaacgcggc cttctggaag tcgcgcagcc gcacgtcgaa ggcctggtac
     3721 gactggaggt attcgtgcgc catcgtgccg atcgccacca gccccatcgt gcgggcgaag
     3781 tggacgttgg acgtgccctt gaagtgctcg ggcgcctcgc gctgcagcgt gcgcacgact
     3841 tcctcgtgcc acgcgcccga gaaacggcgg cggatgccga agtcgaagaa ctcgaacggg
     3901 cagccctccc ggccgccgcg ctcggcgtcg tactcgcgca gcagggcgag cttggcccgc
     3961 aggcgacggc ggccctcctc gatcgccgga acctgctcga cgcggcggaa gtagagctcg
     4021 ttgacgatct cgagcacgta gatctcgaag cccatcacgt gcacctgcgg gccgcgggcg
     4081 acgatctcca gcgtgcgccc gtcggcgccg gcgcgcgcct gcacgaactg gcgctggaag
     4141 tggaagatgc gcaggtagtc gacgaagtcg ctcttcatgt agcgcaggcc gccgaggtag
     4201 gcgacctcgt cgtccgtgaa gcgcagcgag cacaggtggt cgagctgctc gttgacctcg
     4261 tcgacgagca gcgccagcgg gaacgcgggc tggttgcggc acgtgaagcg gtattccgcc
     4321 tgcgccgccg ggtggcggtg cagcatggtc tgccacatcg tgaacttgta gaggtcggtc
     4381 tcgagcaggc tgtggatgac ggggatcatg gcggcgattc tcgtgcgtcg cgaaagacga
     4441 tgggatcagg cggtggcggc gatggcttcg accagggcga gcgcctcggc cgaggtcatg
     4501 agccgcacgc cgagtgcgcg catctgcgcg aagaactcgc cctgctgcgc ctcgaagccg
     4561 ccgaccgggc tcatgcagtc ggtcagcagc acgacgcgtt ccgggcggcc ggagggcagg
     4621 ttggcgacga ggtgctccgt cgtggcgcgg acgcagtggc tggacgcctc gcccgcgacg
     4681 agcagcagct gcgcgcggtc gagcgcctcc aacagggccg tgttcagcag cgtgtgcggg
     4741 tcgtccgcgt cgggcacctc ggcctggatg gcgctgtagt gctcggtcca cgggttctcg
     4801 cccttggtca ccttgcgcac gccgcgcagc gtcgcgcgct cccaggcgtt gtaggcggcg
     4861 cgcacgtcgg cgtggacgtc gtggccccag gtgccgatct cgcagtgcac gggccagacc
     4921 atcagcacgt agcgcccacg ggcctcgagc gcgtcgaggt aggccagcgt gcgcggcagg
     4981 gcggcagcgt cgcgcggcgc gaactcgccg gcgcgcacct gggccgcggt gatcggcgtg
     5041 aacggcgcga ccgcgccgcc gtcgccgcgg cgccagaagg tcgggtgcgc gacgtcgacc
     5101 tggtggtgcg agtcgagcgt gacggtgatc tcgtcgatcg ccggcccggc ggtgcggatc
     5161 agggcggcag tgcgcagcat gtcggcatgc ccaccggcca ccggcagcgc gggggcgacg
     5221 ggtgcgcccg tgagcggatc gagcgggcgg taggcggccg ggacgtcgca gaagtcgttc
     5281 tgcgggtcga tgaccaggag ctggacgttg cgggtcatgg tggtttcctc tggtgtgtcc
     5341 gtggacgcac tatagcgcat gaagttggtc tgtacaactt aattcggtcg actcccgcct
     5401 ggtggcgccc ggcggagatg cttgaccctg cggaattccc tggaatatga agtcaaactg
     5461 aaaagttaaa cttcctttct gcgacaatgg cacggatgaa caagcccggc accggcccaa
     5521 gctccgaccg ccatgcggat cgcctgcacg tcctcgccgc cgacctgcgc gtgctgctgg
     5581 gccgcctgcg ccggcgcctg cgcgagcaga cgggcggcgg cgagctgccg ccggcgcagg
     5641 tggacgtggt gcaccagctc gagcgcgagg gcgcggccac gatcgcgcag ctcgcgcgcg
     5701 cgcagggcat gcggccgcag tcgatgggcg cgaacgtcgc cgcgctggag gcggcgggct
     5761 acgtcgccgg cgcgcccgac ccggccgacg gccgccagac gctgtggtcg ctcacgcccc
     5821 actgcatcgc ggtgatgaag gccggccggg cggcgcgcga ggactggatc gcgcgcaccg
     5881 tccaggatca cttcagcgcc gctgagcagg ccgagctcgc gcgcggcatc gcgctgctca
     5941 agcgcatcgc ggacagttga ggaatcccca tgcccatcac catgctcgat ccccgcaccg
     6001 ccctcgtcgt cgtcgacctg cagaagggca tcgccagcgc gcagacggtc ccgcacgccg
     6061 ccgccgaggt cgtcgcccgc gccgcgcgcc tggccgaggc cttccgtgcg gcggggctgc
     6121 cggtcgtgct ggtcaacgtc gacgggatgg cgccggggcg caccgatccg gcgccgccgc
     6181 ggccaccgtt tccgcccgga tgggacgagc tcgtgccgga gctggacgcg caggcgtccg
     6241 acctgcgggt gaccaagcgc acgtggggcg cgttcgcgca cacgacgctc gaggcccagc
     6301 tgcgcgcgca cggcgtcacg caggtcgtgg tctgcggcat cgcgacgagt gcgggcgtcg
     6361 agtccaccgc ccgcgccgcg cacgagctcg gcttcaacgt gacgctgccg accgacgcga
     6421 tggccgaccg cagcgacgag gcgcacgagg gcagcgtcgc gcgcgtgttc ccgcgcatcg
     6481 gcgagaccgg ccccacggcc gacgtcctcg ccctgctggc gcggcgggag tgacgcgcgg
     6541 atgagcctgc ggtccacctt ccgctcgctg cgcggctaca actaccgcgc ctgggcgatc
     6601 ggcgccttcg tctccaacgt cgggacatgg atgcagcgca ccgcccagga ctggatcgtg
     6661 ctgacggcgc tcaccgccca cagtgcgacg gccgtgggca tcgtgatggc gctgcagttc
     6721 ggcccgactg ccgtgctgct gcccttcgcc ggcttcgccg ccgaccactt cgaccgccgc
     6781 aagctgctgt tctggacgca gggcgcgatg ggggcgctcg cgctcgggct cgggctgctg
     6841 accgtgaccg gcgtggtcac gctgtgggag gtctacgcgt tcgcgctcgc cctgggcgtc
     6901 gccacggcct tcgacgcgcc ggcgcgccag accttcgtct cggagctggt cggcgagtcg
     6961 gacctgtcca acgccgtggc gctgaactcg acctcgttca acgcggcgcg cctgatcggc
     7021 ccggcggtcg cgggcacgat gatccacgtc ctgggctcgg gctgggtgtt cctgctcaac
     7081 gcggcgtcct atgtcgcggt gctggggtcg ctgcgagtgc tgcgcgtcga ggaactgcac
     7141 cgcggcgccc gtgccgcccg cgcccgcgga agcttcgccg aaggcctgcg ctacgtgcgc
     7201 ggccggcccg acctcgtcgc ggtgctggcg atgcttttcc tgatcggtac cttcgggctc
     7261 aacttcccga tcttcctgtc cacgatgtcg gtcatggtct tccacgccgg cgcgcgcgag
     7321 tacgggctgc tgacctcgac gatggcggtc ggctccgtgc tgggcgcgct gctgtcggcc
     7381 ggccaggagc gcccgcgcgc cgcgctgctg ctgggcggcg cggcggcctt cggcatcggc
     7441 tgcgccatcg cggcgctgat gccggactac tggctcttcg gcgcggtgct ggtggcggtc
     7501 gggatctcgg tgcaggtttt caacacgacg gccaacgcca ccatgcagct gtccaccgat
     7561 ccggtgatgc gcgggcgcgt gatggcgctg gtgatggcga tcgccctcgg cggcacgccg
     7621 atcggagcgc cgctggtcgg ctgggtcgcc gacaccttcg ggccgcgctg gagcctgggc
     7681 gtgggagcgg cttccgggct gctcgcggcg gtggtcggcg tgcgctacct cgtgcggcac
     7741 cggcgcctga gcgtccggct ggagtcgggg cggctgcgct tccggctcgc cccgagcgcg
     7801 atcgccgccg cggcggccgc cgtcgaggcc gaggcgtcgc gcttctgagc cttgcgcccc
     7861 gtggccggcc gctcccgtcg cttgccggcg tggcgtaccc gcatccggat cgcggccttc
     7921 agcccggcga cgggcggttt ccagtatcct atcctccccc acaggatacg aagcacggcc
     7981 gagccatgtc cctccatccg tcgcatcccg acatcgtcaa gcgcctgaag cgcgccgagg
     8041 gccacctgcg cagcatcgtc gcgatgctgg aggagggccg cgactgcctc gagatcgcgc
     8101 agcagctgca ggccgtcgaa agcgcggtcg gcaacgccaa gcgcacgctg gtgcacgatc
     8161 acatcgacca ctgcctggag cacgcggtcg agggcgcgag cgcgcgcgag gcgatccgcg
     8221 agttcaaggc gatcacgaag tacctctgat gaacgatctc acctccctcc tgcagcacgg
     8281 cggcaacgcc tggctgttcc tgcccaccgc gatcgtgctg ggcgcgctgc acggcctcga
     8341 gccgggacat tcgaagacga tgatggccgc gttcatcatc gcggtgcgcg gcacgctggg
     8401 gcaggcggtg ctgctcgggc tcgcggccac cgcctcgcac acggccgtcg tgtggctcgt
     8461 cgcgttgggc ggcctgtact tcggccagcg ctgggacgcc gaggcgagcg agccgatcct
     8521 gcagctcgtc tccgccgcga tcatcgtcgg cgtcggcgca tggatggtgt ggcgcacctc
     8581 gcgcaaccag gcggcgtgct tctacgacca cggcgacgcg gcgcacggcc acgaccatgc
     8641 gcacgaccac gcccgcgacg accgcgcgcg tgatcaccgc catgaccacg cccgcgcgca
     8701 cggcccggtg gcgcacgtgc acggcgcggc gtgctcgcat ggcgacgacc acgaccacga
     8761 ccacgaccac gaccacgacc acgaccacga cccggcgccc gtcgccactg cggacgaccc
     8821 ccacggccac ggcgccgaca tccagcgccg cttcgccgga cgccacgtca ccacggcgca
     8881 gatcgtgatg ttcgggctca ccggcggcct gatcccgtgc ccggcgtcga tcacggtgct
     8941 gctgctgtgc ctgcagctca agcggctggc gctcggcgcg acgctggtgc tcggattcag
     9001 catcgggctg gcggcgacga tggtggcctc cggcgtcgtc gcggcgatcg gcgtgaagca
     9061 tctttcgaag cgcgcggatg cctcgggccg gctgtccgca ctggccgcga aggcgccgta
     9121 cctgtcgggc ggactgatcc ttctggtcgg ggcggtgctc gcctggcagg cgctgcgcgc
     9181 gatggggtga gcatgcccgg gtcgacgacg ccccggcttg cccccgccgt cgcggtggcc
     9241 gcgctgcccg acgcgctgcc cgacgcgctg ccttgagcgc cacgcgccgg cgactcgacg
     9301 gggcgcgcgc cttgccgcgg cgaggcatcc cttgccgcga gcccgccatg ccccacgcca
     9361 gccatcaccc gacgaagccg aagtccccgt cgccacaccg ctggtcgcag cacgtcgccg
     9421 agaccagcga cgcgctcgac ctgcccaagg acatgttcaa gcagtccgac ccgaagcgca
     9481 tcgccgcgga cctcaagcgt tcggcggagc acagcagccg ccgccacgcc gagccgtacc
     9541 gctcggcgat gtcgatgctg accttctacg tcaaccgcgc gggcaagacg ctcggcgcga
     9601 cgcagaagcg ccggctcgag gccgccaagg acgagctgcg cgcgctcttc ggccgtccgc
     9661 ggcgcgacgc ctcgtcgtcc ggagcgcgca agtgaagtcg cccggccacc ggcagcatcc
     9721 cgggcaccgg gtgctcgagc gcggcatgcc cggccgcctg caggcgtgcg tcggcggcga
     9781 cgtgctcgcc gaatccgacg atgtcctcga ggtcgacgag gacggcaatc cgccgcgcta
     9841 ctactttccg cgcggcgacg tgcgcgcgca ggcgctgcag cgcacggctt cgccgacgca
     9901 ctgcccgtac aagggcgacg gcgtgctgtt cgcggtgcgc ctgggcgacg gcacgaccgt
     9961 cgccgacggc gcctggagct acgagcagcc ctacgacgag cacgcggcgc tgaaggaccg
    10021 ggtcgcgttc tggagcgaga agttcgagaa catcgagttc aaggccgtgc cctgaccggc
    10081 gtcagaactt gacctgcagg cccacgctcg aggtgaggtc ggactggtac atcgtgccgc
    10141 gcgtcaccga gaagtcgagc tgcgtcgact tcgagctcgc gtaggtcgca cccgcgtgca
    10201 cctgggcggt cgagttctcg atcaggttgc ccggcgagat cttctcgccc gagacctcga
    10261 tgaagctgcg ccagtgcgcg ggcggcgcgt gcggatcgag cgtgctcgcc tgcaggccgg
    10321 cgacgtagtg ctccagcgtg ccgccgccga tgcgctgcac gcccggcgtg aggccgaacg
    10381 tgaggtcgga cagcgagtcc cattgcgcgc tgatgcgcaa cgagcgtcgc accgtgcgcg
    10441 gcgcggggcc ggcgaacagg tctgcctgag tgctcagcgc gatctgcgac agcagcggcg
    10501 cgtcgatgcg gtcctcgctg ccccagcgcg cgccgacctt caccgcgttc ggatcgacct
    10561 gcagcggcag cacccgcacg aacgggtcgc tgctcggcgc cgccgggtcg ggcaggccgt
    10621 cggcatgcac ctgcacgaac tggcccggga tcagccgcag cgtcgcctgc gacgtggcga
    10681 gcgcgctcgg gtcgaccggc cccagcggct tcgggccgcc ggccagcgcc accgccg
//
LOCUS       DASVLN010000876         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_476472, whole genome shotgun sequence.
ACCESSION   DASVLN010000876 DASVLN010000000
VERSION     DASVLN010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476472"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VF453_20620"
     CDS             <1..271
                     /locus_tag="VF453_20620"
                     /inference="COORDINATES: protein motif:HMM:NF042415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STY0301 family protein"
                     /protein_id="HEX7690123.1"
                     /translation="DGVCPLHAGAAPSQIDVYDGDPSEQAILAPDDSTGPGANTYTVK
                     QVYAEGRVVTIRCHYGHDAVDVKLSRPVAACRFSGDEARPQVVCK"
     gene            392..880
                     /locus_tag="VF453_20625"
     CDS             392..880
                     /locus_tag="VF453_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690124.1"
                     /translation="MPVQWSPTWNAADNNDVNPTFGQAQILSIIRSVSPSILRVQPAA
                     VRDAVQLVIADSSDVTVIKGLHHVGTEKIDIKKTSHPAGPVVHLYCMNYSLVDSGLRR
                     PYDDVTRAGSGWRITGSSAFLGDGSNELAGMDKTGEIAELRADGKTKVQAQDAFKEKH
                     YG"
     gene            complement(902..>2178)
                     /locus_tag="VF453_20630"
     CDS             complement(902..>2178)
                     /locus_tag="VF453_20630"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX7690125.1"
                     /translation="ACALAALSAVAGGVRVAQACSGPTPEPTGTETHFPLMYGDRPTV
                     AQLTEIGRRIFFDPSYSASGRQACATCHDPRHAFGPPNAMAVQPGGPALDRFGFRNTP
                     SLRYNHAPIAFTQHFMESEVTLGQDDQGPTGGRTWDGRVDTGHDQALMPLLDPNEMAN
                     GTPEAVVARLQRSAYAQQFRDVLSAPGENVFDDPASAMTWVTVALETFEQNAADFHPF
                     DSKFDAWLNDRATLTPAEARGLALFNDVKKGNCASCHPSTRKTSTDRPPIFTDYGFVA
                     LGVPRNRELPADRDPAFFDLGLCGPLRTDLAARPEYCGLFRTPSLRNVALRRRFFHNG
                     AIRSLREAVEFYATRDTAPQRWYRPDTQGRYLPDDLPPRYLPNLSHEIPFRPLPDGRP
                     RLDAREIDDIVAFLGTLTDGWKPPGGEKLARR"
BASE COUNT          329 a          749 c          807 g          293 t
ORIGIN      
        1 ggacggcgtc tgcccgctgc acgcgggcgc ggcgccgagc cagatcgacg tctacgacgg
       61 cgacccgtcc gagcaggcga tcctcgcgcc agacgacagc accggcccgg gcgcgaacac
      121 ctacaccgtc aagcaggtct acgcggaagg ccgcgtcgtc acgatccgct gccactacgg
      181 ccacgacgcg gtcgacgtca agctgtcgcg accggtcgcg gcctgccggt tctcgggcga
      241 cgaggcgcgt ccgcaggtcg tctgcaagta ggccggggac gcccgccgcg tatccctggc
      301 gacggcccgg acatccgccc cggcgtcatg cggccgcgcg aataatccct cgctcgcgca
      361 acggcgagcg caacaaccag ggagaagcag catgcccgtg caatggtcac ccacgtggaa
      421 cgccgcggac aacaacgacg tcaacccgac gttcggccag gcccagatcc tctcgatcat
      481 ccggtcggtc tcgccgtcca tcctgcgcgt ccagcccgcc gcggtgcgcg acgcggtgca
      541 gctcgtcatc gccgacagct ccgacgtcac cgtcatcaag ggcctgcacc acgtgggcac
      601 cgagaagatc gacatcaaga agaccagcca tccggccggc ccggtcgtgc acctctactg
      661 catgaactac tcgctggtcg actcgggcct gcgccgtccg tacgacgacg tgacgcgggc
      721 cggctcgggc tggcgcatca cgggctcgtc ggccttcctc ggggacggct ccaacgagct
      781 tgccggcatg gacaagaccg gcgagatcgc cgagctgcgc gccgacggca agacgaaggt
      841 gcaggcgcag gacgccttca aggaaaagca ctacggctga cgcggccggg ccgcaggccg
      901 gtcagcgccg cgcgagcttc tcgccgcctg gcggcttcca gccgtcggtg agcgtgccga
      961 ggaaggccac gatgtcgtcg atctcgcgtg cgtccaggcg cgggcggccg tcgggcagcg
     1021 gcctgaacgg gatctcgtgc gagaggttgg gcaggtaccg cggcggcagg tcgtccggca
     1081 ggtacctccc ctgcgtatcc ggtcgatacc agcgttgcgg ggcggtgtcg cgcgtcgcgt
     1141 agaactcgac ggcctcgcgc agcgagcgga tcgcgccgtt gtggaagaag cgcctgcgaa
     1201 gcgccacgtt gcgcagcgag ggcgtgcgga acaggccgca gtactcggga cgggccgcga
     1261 ggtcggtgcg cagcgggccg cacaggccga ggtcgaagaa ggcgggatcg cggtcggcgg
     1321 gcagctcgcg gttgcgcggc acgcccagcg cgacgaagcc gtagtcggtg aagatcggcg
     1381 gccgatcagt cgaggtcttg cgcgtgctcg ggtggcagct cgcgcagttg cccttcttga
     1441 cgtcgttgaa cagcgccagg ccgcgcgcct cggcgggcgt gagcgtcgcc cggtcgttca
     1501 gccatgcgtc gaacttgctg tcgaaggggt ggaagtcggc cgcgttctgc tcgaaggtct
     1561 cgagcgccac ggtcacccag gtcatcgcgg aggccgggtc gtcgaacacg ttctcgccgg
     1621 gcgcgctgag gacgtcgcgg aactgctgcg cataggccga ccgctgcagc cgcgcgacga
     1681 ccgcttcggg cgtgccgttg gccatctcgt tgggatcgag cagcggcatc agcgcctggt
     1741 cgtggccggt gtcgacgcgg ccgtcccagg tgcggccgcc ggtcggaccc tggtcgtcct
     1801 ggcccagcgt gacctcggac tccatgaagt gctgcgtgaa cgcgatcggc gcgtggttgt
     1861 agcgcagcga gggcgtgttg cggaagccga agcggtccaa cgccgggccg cccggctgca
     1921 ccgccatcgc attgggcggc ccgaaggcgt ggcgcgggtc gtggcaggtc gcgcacgcct
     1981 gccggcccga cgcggagtac gaggggtcga agaagatgcg ccggccgatc tcggtgagct
     2041 gggcgaccgt cggccggtcg ccgtacatca gcgggaagtg ggtctcggtg ccggtgggct
     2101 cgggcgtcgg gcccgagcag gcctgcgcga cgcgcacccc gcccgcgacg gcagagagcg
     2161 cggcgagcgc gcacgcca
//
LOCUS       DASVLN010000877         2758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_44051, whole genome shotgun sequence.
ACCESSION   DASVLN010000877 DASVLN010000000
VERSION     DASVLN010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44051"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VF453_20635"
     CDS             complement(<1..513)
                     /locus_tag="VF453_20635"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEX7690126.1"
                     /translation="MTDAAADTVPPAAAPAHAHDHDHAQAHAPLAACPNCGASLRLPG
                     LAPEAVRHCPSCGQETTLHPPSVAEFAHEFVGHYIALEGPLWRTLFLLVRQPGRLTAE
                     YLAGRRRRYVIPLRLYLSASFVLFLVLKLLAPTAAHPGPHAADVPATPAASAAQAAPA
                     ASTNDRVIVRA"
     gene            complement(536..1135)
                     /locus_tag="VF453_20640"
     CDS             complement(536..1135)
                     /locus_tag="VF453_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7690127.1"
                     /translation="MSRPTVDRPLPARLLAASLVAALLAACAPPLRAQAAAAATPASS
                     SAERRNWFDDPFLQATHGLPGCPVPDPPLMTEAEMQHETHWRAERGTSCFRSGRCRLP
                     NAYLYDKEIAPRVVRMIRASGAFEDTTVWVEGQRRWVWLKGCVSTQAQADEMERQVRL
                     VDDVEAVIPQLIVGTAMPPGPPRYRLPPGPVAASAPADR"
     gene            1273..1794
                     /locus_tag="VF453_20645"
     CDS             1273..1794
                     /locus_tag="VF453_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX7690128.1"
                     /translation="MSLSLYAVTVKPMRLTLKNLSAVIAKAEAHCEARKIDPAVFVNG
                     RLYPDMLPFKAQVQIASDTAKGAGARLAGVEIPKFEDTESTFAELQARIDRTVAFLDS
                     LDEAKFEGAEARTVTLQLRSGAREFTGVDYLTTWANPNFYFHVTTAYAILRHGGVEIG
                     KMDYLAGSLPAAK"
     gene            2046..2480
                     /locus_tag="VF453_20650"
     CDS             2046..2480
                     /locus_tag="VF453_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7690129.1"
                     /translation="MRQYIRHPIDVPVEIRIDPHAAATALHAQDISLGGLAVHSAEAV
                     EPGSMIEVRIPYVEPAFEARALVAWCRPRRDGSGHEVGVSFLDAQDVFLARMVAQICY
                     IEDYRKSIAREEGRELSPEEAAGEWIAKYASKFPGAGTGGLH"
     gene            2576..>2758
                     /locus_tag="VF453_20655"
     CDS             2576..>2758
                     /locus_tag="VF453_20655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690130.1"
                     /translation="MFPPFPLSPTSASPISGSRLPRILRVAALSAAVAASCLAAGCGG
                     GSGGATSTETSASTGAG"
BASE COUNT          375 a          978 c         1051 g          354 t
ORIGIN      
        1 ggcccggacg atcacccggt cgttcgtcga cgccgccggt gccgcctgcg cggccgaggc
       61 ggcgggcgtc gccgggacat cggccgcatg cggcccgggg tgcgcggcgg tcggcgccag
      121 cagcttgagc acgaggaaca gcacgaagct cgcgctcagg tacagccgca gcgggatcac
      181 gtagcggcga cggcggccgg cgaggtactc cgccgtcagc cggccgggct gccgcaccag
      241 caggaacagg gtgcgccaca gcggcccctc gagcgcgatg tagtggccga cgaactcgtg
      301 cgcgaactcg gcgaccgagg gggggtgcag cgtcgtctcc tggccgcagc tcgggcagtg
      361 gcgcaccgcc tccggcgcga ggccggggag ccgcagcgag gcgccgcagt tgggacaggc
      421 ggccaggggc gcatgcgcct gcgcatggtc gtggtcgtgg gcgtgcgcgg gagccgcggc
      481 gggcggcacg gtgtcggcgg cggcgtcggt catggcgggc ggcgcggtcg ggttctcagc
      541 ggtcggcggg tgcggaggcg gcgacgggac cgggcggcag gcggtagcgc ggcgggccgg
      601 gcggcatggc ggtgccgacg atcagctgcg ggatcacggc ctcgacgtcg tcgaccagcc
      661 gcacctggcg ctccatctcg tcggcctgcg cctgcgtcga gacgcagccc ttgagccaga
      721 cccagcgccg ctggccctcg acccagacgg tggtgtcctc gaaggcgccc gaggcgcgga
      781 tcatcctgac cacgcgcggc gcgatctcct tgtcgtagag gtaggcgttg ggcagccggc
      841 agcggccgga acggaagcag ctggtgccgc gctcggcgcg ccagtgggtc tcgtgctgca
      901 tttcggcctc cgtcatgagc ggcgggtcgg gcaccgggca gccgggcagg ccgtgggtcg
      961 cctgcaggaa cggatcgtcg aaccagttgc ggcgttcggc ggaggacgag gccggcgtcg
     1021 ccgcggcagc ggcctgcgcc cgcaggggcg gtgcgcaagc cgcgagcagg gcggcgacca
     1081 gggaggcggc gagcaggcgc gccggcaggg ggcgatcgac ggttgggcga gacatggtcg
     1141 ccgatggtag cgccgtgcgc gcgtggtgcg cgcacgggtt cgcgtgttgc gcaaggccat
     1201 ggccgggcag ggcccggcgc gctgcgctct aatgcgcgct ccccgtccca tccaaccgaa
     1261 ccggagaccg ccatgtccct gtccctgtac gccgtcaccg tcaagccgat gcggctgacg
     1321 ctgaagaacc tgtccgccgt gatcgccaag gccgaggcgc attgcgaggc ccgcaagatc
     1381 gatcccgcgg tgttcgtcaa cggccgcctc tatcccgaca tgctgccctt caaggcccag
     1441 gtgcagatcg ccagcgacac cgccaagggc gccggcgcgc ggctggcggg cgtcgagatc
     1501 ccgaagttcg aggacaccga atcgaccttc gccgagctgc aggcccgcat cgacaggacg
     1561 gtcgccttcc tcgattcgct cgacgaggcg aagttcgaag gcgccgaggc gcgcaccgtc
     1621 acgctgcagc tgcgctcggg cgcgcgcgag ttcaccggcg tcgactacct gaccacctgg
     1681 gcgaacccga acttctactt ccacgtgacc acggcctacg cgatcctgcg ccacggcggc
     1741 gtcgagatcg gcaagatgga ctacctggcc ggcagcctgc cggcggcgaa gtaacgtctc
     1801 gacgcaagtt cctgcggcgg ccgctgtcga gccgggaagc ggcccgtagg cacgcggtgc
     1861 cccgcgggac ggccggccgc ggccggccat gcccggatct ggcggcgata tacgggccgg
     1921 atccgggctt gcgggtgacg gatttggcgg aatatcgggg ttgcggggcg aggtctccgc
     1981 gcggtacaac cccccgagcc cgtggcgcga cgcgacgggc gcgcagatcc ggaaagctca
     2041 caggcatgag gcagtacatc cggcacccga tcgacgtccc ggtggagatc cgaatcgatc
     2101 cgcacgccgc ggccaccgct ctgcatgccc aggacatcag cctgggcggc ctggccgtgc
     2161 actcggccga ggcggtcgag ccggggtcga tgatcgaggt ccgcatcccc tacgtcgagc
     2221 cggcgttcga ggcccgggcc ctggtggcct ggtgccggcc gcgacgcgac ggctccggcc
     2281 acgaggtggg cgtcagcttc ctcgacgcgc aggacgtgtt cctcgcgcgg atggtcgcgc
     2341 agatctgcta catcgaggac taccgcaagt ccatcgcccg cgaggaaggc cgcgagctga
     2401 gcccggaaga ggctgccggc gagtggatcg ccaagtacgc ctcgaaattc cccggtgccg
     2461 gcactggcgg cctgcactag cccgccgatt tccctccgta ctgccgacct cggccgccgc
     2521 cggggacggc gcgccgcgcg cgcgccgggc cgtcgccttg cctggagccc atcgcttgtt
     2581 cccgccgttc ccgctcagcc cgacctccgc atccccgatc tccggatccc gcctgccgcg
     2641 catcctgcgc gtggcggccc tgtcggcggc cgtcgccgcg tcctgcctgg ccgccggctg
     2701 cggaggcggg agcggcggcg cgacgtccac cgagaccagc gccagcaccg gggctggc
//
LOCUS       DASVLN010000878         7875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_368067, whole genome shotgun sequence.
ACCESSION   DASVLN010000878 DASVLN010000000
VERSION     DASVLN010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368067"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VF453_20660"
     CDS             complement(<1..1008)
                     /locus_tag="VF453_20660"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX7690131.1"
                     /translation="MTLPDDWRHDREIGVQTTLDTTRVDDVRIGAVRPLISPALLLDD
                     MPAPDSAVSLVERARADIAAVLAGRDDRLVVVVGPCSIHDHDQALEYARHLKVVADAL
                     ADDLLVVMRVYFEKPRTTVGWKGYINDPRLDGSFRINEGLRKARELLLDVAEIGLPAG
                     TEFLDLLSPQYIADLVSWGAIGARTTESQSHRQLASGLSCPVGFKNGTDGGIKVAADA
                     ILAANSAHAFMGMTKMGQAAIFETRGNQDCHVILRGGKTPNYKAADVAAACGALAERG
                     LRQQVMIDFSHANSEKRHERQIAVGTDVAAQIAGGDHRITGVMIESHLADGRQDLKPG
                     VA"
     gene            complement(1115..1723)
                     /locus_tag="VF453_20665"
     CDS             complement(1115..1723)
                     /locus_tag="VF453_20665"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7690132.1"
                     /translation="MIAGLAALAAACGLARAQAPAPAAAASISSSAVAGTYRLDPAHT
                     FATFEVRHFGTSTLRGRFGPLQGEVTVDPAAGTGDVRLRIPMATLSTGFAPLDARLKR
                     PDLLAVDDYPEAYFVATRFRFDAAGGVREVRGEFILRGHSEPLSLYADRFACRDDAML
                     ERRVCGGDFHADLKRGAFGANFGEPFVGDDVHLVIQVEAIAA"
     gene            complement(1846..2427)
                     /locus_tag="VF453_20670"
     CDS             complement(1846..2427)
                     /locus_tag="VF453_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7690133.1"
                     /translation="MKKLTFVAIAALGLAGFANLAQAETATYALDPSHTFATFEISHF
                     GTSTNRGRFNKTEGTVQLDKAARTGKLDVTVHTDSLDTGTAAFTKHISSNEILDVAAF
                     PTAQFTGDKFTFNGDKVTDIAGTLTLHGKSNPVTLHADNFNCYQNPMLKREVCGGDFE
                     ATIVRSQYGVNYGLNYGFPDNVHLVIQVEAVKQ"
     gene            complement(2573..3142)
                     /locus_tag="VF453_20675"
     CDS             complement(2573..3142)
                     /locus_tag="VF453_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7690134.1"
                     /translation="MNQTFRSALVLAAGVAFAGAAFAQAKVIPVQSQIGFAVKEMGVP
                     VEGRFAKWTADVAFDPKKPEAGKVAFTIDTASASFGVKETDAELPKPEWFNVAKFPTA
                     SFASTSIKATGGGKFAVAGKLTLKGTTKDVVVPIALAQAGGTTTATGQFVVKRNDFHV
                     GEGEWADPSQVADEVQVKFRIALTGVGPM"
     gene            complement(3195..3743)
                     /locus_tag="VF453_20680"
     CDS             complement(3195..3743)
                     /locus_tag="VF453_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX7690135.1"
                     /translation="MNNSTASTGRYSGTAIALHWVMALAIVGNFCLGLYMSDLGMSML
                     RLRLFNYHKWTGATILALAALRLLWRLANRPPADVPAPRWQHAAAHATHWALYALFFA
                     VPLTGWAYSSAAGFPLVVFGVLPLPDFVPKDHALAEALKPWHVALAWTLAALVALHLA
                     AVFKHMVIDRDGLVRRMMPGRA"
     gene            complement(3877..4827)
                     /locus_tag="VF453_20685"
     CDS             complement(3877..4827)
                     /locus_tag="VF453_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HEX7690136.1"
                     /translation="MTQEHWPALRLARACGGPARVPFLLASEQPGPARVLGSVAVRDV
                     ERLRAWPDAFAIEVDAGGVPSAVVLVLAPAERDARLAQIHAVLREAGAIVGWRDEPYP
                     LRDRGGGEHATIERAASRFWGTMTLGAHCNGYVVDASGRPGWLWIARRSLTKQTDPGR
                     LDNLIGGGVPRGQSPREAVVREGWEEAGLLPAQMAGLEEGRVIELHADLPEGRQHERL
                     FVFDLELPLGLEPRNVDGEVAEHRRMPVAEALARAAAGELTTDSALVTLDFALRRGLL
                     GTGLEPADARAGSTLRQAFEALLHPAAAVDAGPVIQGTGR"
     gene            4890..4966
                     /locus_tag="VF453_20690"
     tRNA            4890..4966
                     /locus_tag="VF453_20690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4924..4926,aa:Arg,seq:ccg)
     gene            complement(5253..5948)
                     /locus_tag="VF453_20695"
     CDS             complement(5253..5948)
                     /locus_tag="VF453_20695"
                     /inference="COORDINATES: protein motif:HMM:NF020501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PoNe immunity protein domain-containing protein"
                     /protein_id="HEX7690137.1"
                     /translation="MQPNYFVERLRYIDETIDSRLAKLRDQPDVYKKPQAFLRANLRE
                     RCEQIFLGYSLGESLGQLAKRFPAVVEAYEAHVQCPGYVPHDFADLDRYLVSLWLVSF
                     GIIFDVDDELWRRLLVCIGNEGRDALYERLVEVRTPGRKAAPGLVHSKIFQPLFEAIA
                     AEGERRAELIRQYLGNWYKALKNAYWIDTHARSDGGFFGYWAVEVAGVVSAFDMDDSS
                     FRGMPYYPRDLVA"
     gene            complement(5992..7329)
                     /locus_tag="VF453_20700"
     CDS             complement(5992..7329)
                     /locus_tag="VF453_20700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690138.1"
                     /translation="MPQGLGTSGSSRSPAPADYASQHLTLAEIQHRAAVLAHEVSTAV
                     GWHETHSVVWRFLGGHSFTAGVVYGLFENFAHSVIGAADMLKTFALAEYWESQHGGSN
                     AAQIRTRLSMATLPVIGPSLAIASTLAARFHINSLEQKAKEAYEEREAIVEAVKYVFT
                     NPKDFLGKVTTSQVARFNEFKKYMAEPSLASSFEAGKLLGELLFDVLMVIDLAAGLAK
                     LALQVPKLARFTEDFAKLAGDLRTAKRLEKAVKDSELVEPPPKAERGAANQTSGRSSS
                     TNRSASPPPPPPPPVDDAPPVDQAPDDPQDFTRNEKGVYGEAQSDNYMQDEGFQKLNG
                     DIVKVGDPPLGPGIDGVWRNTAPPPEYVITESKYGSSTLSTLKDGTKQMSDDWVDQRL
                     NKAVGEQTANAIRDADARGQVEKWLLQVDENGNVSKSLVDADGNIVDPTIPGG"
     gene            complement(7606..>7875)
                     /locus_tag="VF453_20705"
     CDS             complement(7606..>7875)
                     /locus_tag="VF453_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7690139.1"
                     /translation="DPAVVPEAARPGGWLGERADGAGALAYFRWHGSPRMYRSRYDDA
                     WLHARAAGIARLPALARVWCVFDNTGAGEAMGNALALAAMVGAEG"
BASE COUNT         1180 a         2667 c         2784 g         1244 t
ORIGIN      
        1 cgcgacgccc ggcttcaggt cctggcggcc gtcggcgaga tggctctcga tcatcacgcc
       61 cgtgatgcgg tggtcgccgc cggcgatctg ggccgcgacg tccgtgccga cggcgatctg
      121 gcgctcgtgc ctcttctcgg agttggcgtg cgagaagtcg atcatcacct gctggcgcag
      181 gccgcgctcg gccagcgcgc cgcaggcggc ggcgacgtcc gcggccttgt agttgggcgt
      241 cttgccgccg cgcaggatca cgtggcagtc ctggttgccg cgcgtctcga agatcgccgc
      301 ctggcccatc ttggtcatgc ccatgaaggc gtgcgccgag ttggcggcca ggatcgcgtc
      361 ggccgcgacc ttgatgccgc cgtcggtgcc gttcttgaag ccgaccgggc acgacaggcc
      421 cgacgccagc tggcggtggc tctggctctc ggtggtgcgc gcgccgatcg cgccccagga
      481 cacgaggtcg gcgatgtact gcgggctcag caggtcgagg aactcggtgc cggcgggcag
      541 gccgatctcg gcgacgtcca gcagcagctc gcgggccttg cgcaggccct cgttgatgcg
      601 gaagctgccg tccaggcgcg ggtcgttgat gtagcccttc cagccgacgg tggtgcgcgg
      661 cttctcgaaa tagacgcgca tcacgacgag caggtcgtcc gcgagcgcgt cggcgacgac
      721 cttcaggtgg cgcgcatatt ccagcgcctg gtcgtggtcg tggatcgagc agggcccgac
      781 gacgacgacc agccggtcat cgcgccccgc cagcacggcg gcgatgtcgg cgcgcgcgcg
      841 ctcgaccagc gagacggcgc tgtccggcgc gggcatgtcg tcgagcagca gcgccggaga
      901 gatcagcggg cgcacggcgc cgatgcggac gtcgtcgacg cgcgtggtgt cgagggtggt
      961 ctggacgccg atctcgcgat cgtgccgcca gtcgtcaggg agggtcatcg ccgggctttc
     1021 ggtggacggg acgggtcggc cgccgcaggg ggcggaccgt cgcgattatc gggcaggcgg
     1081 gaaaacccgg ccgcggccgc gcggcgcccg gcgatcaggc ggcgatcgcc tcgacctgga
     1141 tgaccaggtg cacgtcgtcg ccgacgaacg gctcgccgaa gttggcgccg aacgcgccgc
     1201 gcttcaggtc ggcatggaag tcgccgccgc acacgcggcg ctcgagcatc gcgtcgtcgc
     1261 ggcaggcgaa gcggtccgcg tagagcgaca gcggctcgct gtggccgcgc aggatgaact
     1321 cgccgcgcac ttcgcgcacg ccgccggcgg cgtcgaagcg gaaccgggtc gcgacgaaat
     1381 acgcctcggg gtagtcgtcg accgcgagca ggtccggccg cttgaggcgc gcgtccagcg
     1441 gcgcgaagcc ggtcgacagc gtcgccatcg ggatgcgcag ccgcacgtcg cccgtgccgg
     1501 cggcggggtc gaccgtcacc tcgccctgca gcggtccgaa gcggccgcgc agcgtggagg
     1561 tgccgaaatg ccgcacctcg aaggtcgcga acgtgtgcgc ggggtccagc cggtaggtgc
     1621 ccgcgaccgc cgacgacgag atggacgccg ccgccgcggg cgcgggtgcc tgcgcgcggg
     1681 cgaggccgca cgccgccgcg agggcggcaa gcccggcgat cacggcacga gcgggccaat
     1741 gcgtccatcg ccgggcgccc gcgctcggct gccgtcctcg gccgctcctt cgccaaggcc
     1801 ggcgccctct cggcgggctg cctgcggccg gccgccggcg ggggctcact gcttgacggc
     1861 ctcgacctgg atcaccaggt gcacgttgtc ggggaagccg tagttcaggc cgtagttcac
     1921 gccgtactgg ctgcgcacga tggtcgcctc gaagtcgccg ccacagacct cgcgcttgag
     1981 catcgggttc tggtagcagt tgaagttgtc ggcgtgcagc gtcaccgggt tggacttgcc
     2041 gtgcagcgtc agcgtgccgg cgatgtcggt gaccttgtcg ccgttgaacg tgaacttgtc
     2101 gcccgtgaac tgcgccgtcg ggaaggcggc gacgtcgagg atctcgttgc tggagatgtg
     2161 cttggtgaag gccgccgtgc cggtgtcgag cgaatcggtg tgcacggtca cgtccagctt
     2221 gccggtcctg gcggccttgt ccagctgcac ggtgccttcc gtcttgttga agcggccgcg
     2281 gttggtcgag gtgccgaagt ggctgatctc gaaggtcgcg aaggtgtgcg acgggtcgag
     2341 cgcgtaggtc gcggtctcgg cctgggcgag gttggcgaag ccggcgaggc cgagggcggc
     2401 gatggcgacg aaggtgagct ttttcatggg gaggactcca gggtcgttgg cgttggaaag
     2461 aggatgccgc ggcgctgatc cgcgccgccg gcaggcgccc ctcgcggggc gcgttgtgag
     2521 cgattcgtga ggatggcagg gttggcgttc gcgccgcgca cggcgcgccc ggtcacatcg
     2581 ggccgacgcc ggtcagtgcg atccggaact tcacctgcac ctcgtcggcg acctgcgagg
     2641 gatcggccca ctcgccctcg ccgacgtgga aatcgttgcg cttgaccacg aactggccgg
     2701 tcgcggtggt cgtgccgccc gcctgcgcga gcgcgatcgg caccacgacg tccttggtcg
     2761 tgcccttgag cgtcagcttg ccggcgacgg cgaacttgcc gccgcccgtg gccttgatcg
     2821 aggtcgacgc gaagctcgcg gtcggaaact tggccacgtt gaaccactcg ggcttgggca
     2881 gttcggcgtc ggtctccttg acgccgaagg acgcggaagc ggtgtcgatc gtgaacgcca
     2941 ccttgccggc ctcgggcttc ttcgggtcga aggcgacgtc cgccgtccac ttggcgaacc
     3001 tgccctccac gggcacgccc atctccttga ccgcgaagcc gatctggctc tgcaccggga
     3061 tcaccttggc ctgtgcgaag gcggcgccgg cgaacgcgac gccggcggcg aggacgaggg
     3121 cggagcggaa tgtctggttc atcgcggatg cttcctgggg tatcggagaa gatgcggtgg
     3181 gggggtatcg gacgtcaggc gcggccgggc atcatgcggc gcacgagacc gtcgcggtcg
     3241 atgaccatgt gcttgaagac ggcggccagg tgcagggcga ccagcgcagc cagcgtccaa
     3301 gccagcgcca cgtgccaggg cttgagcgcc tcggcgagcg catggtcctt gggcacgaag
     3361 tccggcagcg gcaggacgcc gaagaccacc agcgggaagc ccgccgcgga gctgtacgcc
     3421 cagccggtga gcggcaccgc gaagaacagc gcgtacagcg cccagtgcgt cgcgtgcgcc
     3481 gcggcgtgct gccacctggg ggccggcacg tcggcgggcg ggcggttcgc caggcgccag
     3541 agcagccgca gcgcggccag cgcgaggatc gtcgcccccg tccacttgtg gtagttgaac
     3601 aggcgcagcc ggagcatcga catgcccagg tcgctcatgt acaggccgag gcagaagttg
     3661 ccgacgatcg cgagcgccat cacccagtgc agggcgatgg cggttcccga atagcggccg
     3721 gtcgaggcgg tggagttgtt catcgtggtt ccggtggagt ctgccggcgc gcggcgcgtc
     3781 ggcatcggct tcatcgtgga cgccagtgtc ccggcagtcg cggcggtgcg ggttgagggc
     3841 gattgacgcc ccgcttcgaa acgtttgaat ggtaggtcag cgaccggttc cctggatgac
     3901 gggcccggcg tcgaccgcgg ctgccggatg cagcagcgcc tcgaacgcct ggcgcagcgt
     3961 ggatcccgcg cgggcgtccg ccggttcaag gcccgtcccg agcaggccgc ggcgcagcgc
     4021 gaagtcgagc gtgacgagcg cggagtcggt ggtcagctcg cccgcggcgg cgcgcgccag
     4081 cgcctcggcg accggcatcc gccggtgctc cgcgacctcg ccgtcgacgt tgcgcggctc
     4141 gagcccgagc ggcagctcga ggtcgaagac gaagaggcgc tcatgttggc gtccctcggg
     4201 caggtcggcg tggagctcga tcacgcgtcc ctcctcgagg cccgccatct gcgccggcag
     4261 gagcccggcc tcctcccagc cttcgcgcac gacggcctcg cgcgggctct ggccgcgcgg
     4321 cacgccgcca ccgatcaggt tgtcgaggcg gccggggtcg gtctgcttgg tgagggagcg
     4381 ccgcgcgatc cacagccacc ccggacggcc agaggcgtcg accacgtagc cgttgcagtg
     4441 cgcccccagc gtcatcgtgc cccagaagcg gctcgcggca cgctcgatcg tcgcatgctc
     4501 gccgccgccc cggtcgcgca gcggataggg ctcgtcgcgc cagccgacga tcgcgccggc
     4561 ctcgcgcagc accgcatgga tctgcgcgag gcgcgcgtcg cgctccgccg gcgcgagcac
     4621 cagcaccacc gccgacggca cgccgcccgc gtcaacctcg atcgcgaacg cgtcgggcca
     4681 cgcgcgcagc cgctcgacgt cccgaacggc gaccgagccc agcacccgcg ccggccccgg
     4741 ctgctccgac gccagcagga acggcaccct cgccggcccg ccgcacgcac gcgccagcct
     4801 cagcgccggc caatgttcct gcgtcatcgc gcgagtctag cggcccgccc gccacccgcc
     4861 accgcgatcg gctaaactac gccgcttgcg cgcccgtagc tcagctggat agagtactgc
     4921 cctccgaagg caggggtcgg acgttcgaac cgtctcgggc gcgccattcg aatcaagtac
     4981 ttgggccacc ttcgggtggc ctttgtgttt gcattaggaa gaatgtaggc aaaacaccca
     5041 cgatcggggg cggacgatgg accgccgttt ccctgccgtg acagggcgca agagcagcac
     5101 tttgccgccc ttgcacagct cgcgcccgac gcccagtgcc cgccaaaggt cgcaccaagc
     5161 gaccagcagt gagccttacc gttggctatc agagacgcag catggcgccg ggttgctgcc
     5221 ctctggggca gagggacggg aagaggccca cctcacgcca ccagatcgcg cgggtagtac
     5281 ggcatgcccc ggaaggagga gtcgtccatg tcgaacgcac tgacgacgcc cgcgacttcc
     5341 actgcccaat atccgaagaa tcccccgtct gaccgtgcgt gggtgtcgat ccagtaggcg
     5401 ttcttgagag ccttgtacca gttcccgaga tactgccgga tcagttcggc ccgacgctcg
     5461 ccctcggccg caatggcctc gaagagtggc tggaaaatct tcgagtgcac aagtcctggc
     5521 gccgccttgc ggcccggggt tcggacttcg accagtctct cgtagagggc atcgcgacct
     5581 tcgtttccga tgcacacaag caggcggcgc cacagttcat cgtcgacatc gaagatgatg
     5641 ccgaagctga ccagccatag cgagacgagg taccgatcca ggtcagcgaa gtcgtgaggc
     5701 acatagcccg gacactggac gtgcgcttcg taggcctcga ccacagcagg aaaccgcttc
     5761 gccaactgcc cgaggctttc accgaggctg tagcccagaa agatctgttc gcagcgttct
     5821 cgaaggttgg ctcgcagaaa cgcctgcggc ttcttgtaaa cgtcgggctg gtcgcgcagc
     5881 ttcgcgaggc gggaatcgat cgtttcatcg atgtaccgca aacgctcgac aaagtagttg
     5941 ggctgcatca aaggctcacg cttattcatc tcaagggcac tccgcgccat gtcaaccgcc
     6001 agggatcgtc gggtcgacga tgtttccgtc cgcgtcgact agcgacttgc tgacgttccc
     6061 attctcatcg acctgcagca gccatttttc cacttgtccc ctcgcatccg cgtcgcgaat
     6121 cgcgttcgcc gtctgctcgc caactgcctt gttcagccgc tggtcgaccc agtcgtcgct
     6181 catctgcttg gtgccgtcct tgagcgtgct gagagtcgag ctgccgtatt tcgactccgt
     6241 gattacgtac tcgggcgggg gcgccgtgtt cctccagacg ccatcgatcc ccgggccgag
     6301 cggtggatcg ccgaccttca cgatgtcgcc gtttagcttc tggaagcctt cgtcctgcat
     6361 gtagttgtcg gactgggcct cgccatagac gcccttctcg ttgcgcgtga agtcctgcgg
     6421 gtcgtccggg gcttgatcga ccggcggcgc atcgtccacg ggcggcggcg gaggaggagg
     6481 cggcgaggcc gagcggttcg tgctgctgga gcgaccagac gtttggttcg ccgcgcccct
     6541 ctcggccttc ggcggcggct cgaccagctc cgagtccttg accgcctttt ccagccgctt
     6601 ggccgtgcgc aagtcccccg cgagcttggc gaagtcctcg gtgaaccgtg ccagctttgg
     6661 aacctgaagg gccagcttgg cgaggcccgc cgcgaggtcg atcaccatca gcacgtcgaa
     6721 cagcagttcg cccaggagct tgccggcctc gaagctgctg gcgagcgacg gctcggccat
     6781 gtacttcttg aactcgttga agcgagccac ctgggaggtc gtcactttgc cgaggaagtc
     6841 cttggggttc gtgaagacgt acttgaccgc ctcgacgata gcctcgcgct cctcgtaggc
     6901 ttccttggcc ttctgctcca gcgagttgat gtgaaaccga gctgccagcg tcgaggcgat
     6961 tgccaaggac ggccctatga ccggcagagt cgccatggac agccgggtgc gaatttgggc
     7021 cgcgttcgag cctccgtgct gactctccca atactcggcc agggcgaagg tcttgagcat
     7081 gtcggccgcg ccgatgaccg agtgcgcgaa attctcgaag agcccataca ccacgccagc
     7141 ggtgaagctg tggccgccga ggaaccgcca gaccacgctg tgggtctcgt gccaacccac
     7201 ggctgtggag acttcgtgcg ccaaaacggc cgcgcggtgc tggatttcgg ccagggtcag
     7261 gtgctgggat gcgtagtccg ccggcgcagg ggatcgactg gagccgctgg tgccaaggcc
     7321 ttgcggcaag gcgaaagtgg agtgatcgta ttgacccagg tttccgggcg gcctgctcgg
     7381 catggaatgg tggaggcgat gacgcgaaag ccgcgaccgt acctcagcag ataccccgtg
     7441 acctatcccc aaggaaaggg atgtgcacgc gaacgtcgcg cccggaaggg gttgcctgcg
     7501 gcctcggcca agggcagttg gaagcctgcg gcggctggga cctcgctaaa gcaccacagt
     7561 tgcacatgac cggattccct gcgcgctggt cccaccaacc cacgctcagc cttccgcgcc
     7621 caccatcgcc gccagcgcga gcgcattgcc catcgcctcg cccgcccccg tgttgtcgaa
     7681 cacgcaccac acccgcgcca acgccggcag gcgggcgatc ccggcggcgc gggcgtgcag
     7741 ccacgcatcg tcgtagcgcg acctgtacat gcgcggcgat ccgtgccagc ggaagtaggc
     7801 gagcgcgccg gcaccgtcgg cgcgctcgcc gagccagccg ccgggccggg cggcctcggg
     7861 cacgacggcc ggatc
//
LOCUS       DASVLN010000879         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_156988, whole genome shotgun sequence.
ACCESSION   DASVLN010000879 DASVLN010000000
VERSION     DASVLN010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156988"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VF453_20710"
     CDS             complement(<1..950)
                     /locus_tag="VF453_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690140.1"
                     /translation="MNSPAWWRVACLSMALVSPSAGAAADGAPASPAAPASALERDLL
                     VSGGSVKVDQPVAGDLIVLGGDVEVAAPVAGDVLAAGGSVRIEADVGRSVVSAAGSLL
                     VDGKVARNVRAAGGQVVLGPRAEIGRNVAAAAGELEVRGPVLGGLQASAGHLLLDGPV
                     SGDVVARADKAELGPGAHLAGSLQLGSDKPMLRSPQAVVAGGVRQLPRPQSDVRPADD
                     RWGGFWPIGLIVVAWLLLFAFPKVVDGSSRSLARQPGRCLLYGAGWLVGAPVLLLVVF
                     ATVLGIPLGLLGLAIWLAGWPVAVAVAGIGAGDAVLARWFP"
     gene            1246..>2384
                     /locus_tag="VF453_20715"
     CDS             1246..>2384
                     /locus_tag="VF453_20715"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7690141.1"
                     /translation="MNDTQVIDTLHALASIPLGAGGLPRLLDEVTEAAIEMAEADLGD
                     LRLLDPASSHLRIAAQRGFPARWIEFWNAAPDGGTCGQALQARRRVIVEDIFHSPSHG
                     DERTHGILLQAGVRAVQSTPLVTRSGQVLGIVSTYMRQPHRPAPRTLRWLDLLARQAG
                     DLVENVSLTAARHDAVRRFGALVAATPGVAWSTGPDWSRIDALKGPPCAAAAQALDRD
                     WLEHCVHPADRSRASVAIDAAIRARGPFGLQLRTRRADGTSGWSRSRAAPVLDAQDRI
                     VEWIGTVVNATESRQARDPPVHERERLRSVLEALPVGVFLADSADCTRVSGNAALLAW
                     LGANEGDNLSPEAPEPDAYGRRIAYAEAGKPLAAADLPMQRAAREG"
BASE COUNT          339 a          935 c          846 g          264 t
ORIGIN      
        1 gggaaccagc gcgccagcac ggcgtcgccg gcgccgatgc cggcgaccgc gacggcgacc
       61 ggccagcccg cgagccagat cgccaggccc agcagcccga gcgggatgcc gagcacggtc
      121 gcgaacacca ccagcagcag cacgggcgcg ccgacgagcc agcccgcgcc gtacaggagg
      181 catcggccgg gctgccgggc gaggctgcgc gagcttccgt ccacgacctt cgggaacgcg
      241 aacagcagca gccaggcgac cacgatcagg ccgatcggcc agaagccgcc ccaccggtcg
      301 tcggcgggcc gcacgtcgga ctgcggccgg ggaagctggc gcaccccgcc cgcgacgacg
      361 gcctgcgggc tgcgcagcat cggcttgtcg ctgcccagtt gcagcgatcc cgccaggtgc
      421 gcgccgggac cgagttcggc cttgtcggca cgcgcgacga catcgccgga caccggaccg
      481 tccaggagca ggtgtccggc gctcgcctgc agccccccga gcaccggccc gcgcacctcg
      541 agctcgcccg cggcggccgc gacgttgcgc ccgatctcgg ctcgcgggcc cagcaccacc
      601 tgcccgcccg ccgcgcgcac gttgcgcgcg accttgccgt cgacgagaag gctccccgcc
      661 gcggagacga cggagcgccc gacgtccgcc tcgatcctca cgctgcctcc cgccgccagc
      721 acgtcgcccg cgacgggcgc cgccacctcg acatcgcccc ccagcacgat gaggtcgccg
      781 gcgaccggct ggtcgacctt gaccgagccc ccggacacga ggagatcgcg ctccagtgcg
      841 gacgcagggg ccgcaggcga cgccggcgcg ccgtccgcag ccgcgccggc ggagggcgac
      901 acgagcgcca tcgacaggca ggcgactctc caccaggccg ggctgttcat gcaaggctcc
      961 ttcggtgacc tcccgcgatc ctgccgtccg ccgacctgcg ggcattgcgt cccctcaacg
     1021 cgacgccgaa gtccgcggcc tcgagcgcac tcgccaccga atcagtcccc gaagcgaaca
     1081 tggggcttga tgatgagctc ggagcgtgcg tgtaatcgcg gttgcgcggc ctcatggcca
     1141 tcgccgagcc ggggccggca aaccgtcgtc cgattcgggc gcgtccacgt ggcgcgctcg
     1201 ccgcgcccga ggacgaccgc atgaagaggc gggaggaacg accagatgaa cgacacgcag
     1261 gtcatcgaca cgctccatgc gctcgcatcg atcccgctgg gagccggcgg cttgcccagg
     1321 ctgctggacg aggtcaccga ggccgcgatc gagatggcgg aagccgacct cggcgacctt
     1381 cgattgctcg acccggcctc gtcgcacttg cgcatcgcgg cgcagcgcgg ctttcccgcg
     1441 cggtggatcg agttctggaa cgccgcaccg gacggcggca cgtgcggcca ggcgctgcag
     1501 gcacgcaggc gggtgatcgt ggaggacatc ttccacagcc cgagccacgg ggacgagcgc
     1561 acgcacggca tcctgctgca ggccggcgtg cgcgcggtgc agtccacgcc gctggtcacg
     1621 cggtcgggcc aggtgctggg catcgtctcg acctacatgc gccagccgca ccgacccgcg
     1681 ccgcgcacgc tgcgctggct ggacctcctg gcccggcagg cgggcgacct cgtcgagaac
     1741 gtctccttga cggcggcacg gcacgacgcg gtccgtcgct tcggagcgct cgtcgcggcg
     1801 acgcccggcg tcgcctggag cacggggccg gactggtccc gcatcgacgc gctcaagggc
     1861 ccgccctgcg cggccgcggc gcaggccctc gaccgcgatt ggctcgagca ctgcgtccac
     1921 ccggccgacc ggtcgcgcgc gtccgtggcc atcgacgccg cgatccgcgc gcgcgggccg
     1981 ttcggactgc agctgcgaac gcgccgggcg gacggcacgt cgggatggtc gcgatcgcgc
     2041 gcggcgcccg tcctcgacgc gcaggaccgg atcgtcgaat ggatcggcac cgtcgtcaac
     2101 gcgaccgagt cccgccaggc ccgcgatccc cccgtccacg aacgcgagcg cctgcgctcc
     2161 gtgctggaag cgctgccggt gggcgtcttc ctcgcggatt ccgcggactg cacgcgcgtc
     2221 tcgggcaacg cggccctgct cgcctggctc ggcgcgaacg agggcgacaa cctgtcgccg
     2281 gaagcgcccg agcccgacgc ctacggccgg cgcatcgcct acgccgaggc gggcaagccg
     2341 ctggccgccg cggacctgcc catgcaacgc gccgcgcgcg aggg
//
LOCUS       DASVLN010000880         4854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_261544, whole genome shotgun sequence.
ACCESSION   DASVLN010000880 DASVLN010000000
VERSION     DASVLN010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261544"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1420
                     /locus_tag="VF453_20720"
     CDS             <1..1420
                     /locus_tag="VF453_20720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7690142.1"
                     /translation="FGGVVLAGYAIAAGAGPFAIGLLAAVPYIVQALQLPATVLVERH
                     GRRKRLAVPLLAGARVIILLIALLPLWPASRATIPLLLLGKFGICALSAVAACGINSW
                     MHQLLSGQPLGSFFAKRLVAGTVLGCVFTLGVGWLLDHPPRGRADLAYAIAFAGSGVA
                     GLVSCAWLARCPEPRMAPAGPVVRARDKLAAPFRDRNYRRLLVMLGAWNFASNFAAPF
                     LTVYLIQQLGVGMGTVTELWVVNQVANALTMFAWGRVSDRLSNKAILSVALPVFFLCT
                     VGLVFARAGAPFGLEMALLVLVHAAMGVVGGGIGLATGNLGIKLAPPGEGTSYLSAVG
                     LVSSAAGGLAPLVAGAVGEWLQSSQFALVLRWVSNATTHELSVLRFEHFEFLFAIAGL
                     LGLYVMHALSRLEEGEEVSERRVVQELLLEAQRSVDQLSSVGSLLSSVFSFDRLSERR
                     LWFRRWRAADTPDARGGARPAG"
     gene            1489..4368
                     /locus_tag="VF453_20725"
     CDS             1489..4368
                     /locus_tag="VF453_20725"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX7690143.1"
                     /translation="MTPPDPASTEPAPTADAQGATEAGYALIPVVGLGGSAGAIEALE
                     GFLRAARPDAGFAYVVILHLSPVHESALAEVLQCHTSMPVRQVSRQTRVERDTIYVIP
                     PGHALQMNDGLIRLAPLPRDKPGRVAVDLFFRTLADSHGPHAVAIVLSGMDGDGATGI
                     KRIKERGGLTIAQAPEEALHDDMPRAAIATGMVDWILPVEEMPLRVQRYLELEVRLHL
                     ARQQEADASRAEGSTITERELREILASLRTRTGRDFSNYKRATMLRRIGRRMQINGTP
                     TVADYLACLRTRPGESGALQQELLISVTNFFRDPECFDALAREIPAMLDRAEGTLRVW
                     VPACATGEEAYSIAMLLAEHSHALASPPAVQIFATDLDEEAIRIARDGLYPDTIATDV
                     SQERLRRFFVREHQGWRVRRELRERILFAEHDLLKDPPFSRLDLVSCRNVLIYLNRDG
                     QRRVLEVAHFALRPQGRLFLGASETVEDHHALFYPLDKKHRVFAARQLPRSHLPTLAG
                     PGILARALDLHEPARASNGLPHAAAQAADAAPSAIDARPARGPGSWEGRPASWAELHF
                     KMLEMFAPPSIVIDGAHEIMHVSASAGRFLHLGGGEPTRNLLQLIHPGLRSELRAAIY
                     KAGHATGRAELAPTPIEIGGQVLLVKAVVAAMADVAPGMMLVTLDAQPAGDAAGLAAA
                     DGAQEDGDALATLLERELDGLKSQLRYTVEQYEASTEELKASNEELQAMNEEMRSATE
                     ELETSREELQSINEELTTVNHDLKGKVEELGRANGDLQNLMDATAIATVFLDRELCIA
                     RYTPTAVSLFNLIPTDVGRPLSHMTNRLDYPQLEADARRALQELVPVEREVADADGRR
                     YLARTRPCRTGEDRISGVVLTLIDVTERKRSQEALQLSEERFGAIGAALREPLAAAER
                     AAAALASPDACSDADRAQAPALLQRQLARMRGLLDELLDRPRPAT"
     gene            4563..4790
                     /locus_tag="VF453_20730"
     CDS             4563..4790
                     /locus_tag="VF453_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690144.1"
                     /translation="MEARIAFAERVLRDQQAIVRRLTSQGYCAQLAHELEANIRDSLD
                     ILVRRREDIVAAMQATDAPTPSTPPDTLLRR"
BASE COUNT          629 a         1778 c         1761 g          686 t
ORIGIN      
        1 gttcggcggc gtcgtgctgg cgggctacgc gatcgcggcc ggcgccgggc cgttcgcgat
       61 cgggctgctc gcggccgtgc cctacatcgt gcaggcgctg cagctgccgg cgacggtgct
      121 ggtcgaacgc cacggccgcc gcaagcggct ggccgtgccg ctgctggccg gcgcgcgcgt
      181 gatcatcctg ctgatcgcgc tgctgccgct gtggccggcg agccgcgcga cgatcccgct
      241 gctgctgctc ggcaagttcg gcatctgcgc gctgtcggcg gtcgcggcct gcgggatcaa
      301 ctcgtggatg caccagctgc tgtccgggca gccgctgggc tcgttcttcg cgaagcgcct
      361 ggtcgcgggc acggtgctcg gctgcgtctt cacgctgggc gtgggctggc tgctcgacca
      421 cccgccgcgc ggccgcgccg acctcgccta cgcgatcgcg ttcgcgggct cgggcgtcgc
      481 cgggctcgtg agctgcgcct ggctcgcgcg ctgccccgag ccccgcatgg cgcccgcggg
      541 cccggtcgtg cgcgcgcgcg acaagctcgc ggcgccgttc cgcgaccgca actaccgccg
      601 cctgctcgtg atgctgggag cctggaactt cgcgagcaac ttcgccgcgc ccttcctcac
      661 ggtctacctg atccagcagc tgggcgtcgg catgggcacc gtgaccgagc tgtgggtcgt
      721 caatcaggtc gccaacgcgt tgacgatgtt cgcgtggggg cgggtgtcgg accggctgtc
      781 caacaaggcc atcctgtcgg tcgcgctgcc cgtgttcttc ctgtgcacgg tcggcctcgt
      841 gttcgcgcgc gccggcgcgc cgttcggcct ggagatggcg ctgctggtgc tcgtgcacgc
      901 ggccatgggc gtggtcggcg gcggcatcgg cctggcgacg ggcaacctcg gcatcaagct
      961 cgcgccgccg ggcgagggca cgtcctacct gtcggcggtg gggctcgtct cctcggccgc
     1021 cggcggcctg gcgccgctgg tggccggcgc ggtgggcgaa tggctgcaat cgagccagtt
     1081 cgcgctcgtg ctgcgctggg tctcgaacgc gacgacgcac gagctgtccg tgctgcgctt
     1141 cgagcacttc gagttcctgt tcgcgatcgc gggcctgctc gggctgtacg tgatgcatgc
     1201 gctgtcgcga ctcgaggagg gcgaggaggt cagcgagcgc cgcgtcgtcc aggagctgct
     1261 gctggaggcg cagcgcagcg tcgaccagct gtcctcggtg ggcagcctcc tgagcagcgt
     1321 gttctcgttc gaccgcctct cggagcgccg cctctggttc cggcgctggc gcgcggccga
     1381 tacccccgat gcgcgaggag gcgcgcgccc ggcgggatga ggtgcggatg gcgcacaatc
     1441 tgcgaagctc ggccgcgcca gccgacgcca ttccctggag ccgccgcctt gaccccccca
     1501 gatcccgcct cgactgagcc cgcgccgact gccgatgcgc agggcgccac cgaggccggc
     1561 tacgcgctga tccccgtggt cgggctcgga ggctcggcgg gcgcgatcga ggcgctggag
     1621 ggcttcctgc gcgccgcgcg tcccgatgcc ggcttcgcct acgtcgtgat cctgcacctg
     1681 tcgccggtgc acgagagcgc gctcgccgag gtccttcagt gccacacgtc gatgccggtg
     1741 cgccaggtgt ccaggcagac gcgcgtggag cgcgacacga tctacgtcat cccgccgggc
     1801 cacgcgctgc agatgaacga cggcctcatc cgcctcgcgc ccttgccgcg cgacaagccc
     1861 ggccgcgtgg cggtcgacct gttcttccgc accctcgccg actcgcacgg cccgcacgcc
     1921 gtggcgatcg tgctgtccgg catggacggc gacggcgcca ccggcatcaa gcgcatcaag
     1981 gagcgtggcg gcctgacgat cgcgcaggcg cccgaggagg cgctgcacga cgacatgccg
     2041 cgcgccgcga tcgcgacggg catggtcgac tggatcctcc cggtcgaaga gatgccgctg
     2101 cgcgtgcagc gctacctcga gctcgaagtg cggttgcacc tggcgcgcca gcaggaggcg
     2161 gacgcatccc gggccgaggg ctcgacgatc accgaacgcg agctgcgcga gatcctcgcg
     2221 agcctgcgca cccgcacggg gcgcgacttc tccaactaca agcgcgcgac gatgctgcgc
     2281 cgcatcgggc ggcgcatgca gatcaacggg acgcccaccg tcgcggacta cctcgcctgc
     2341 ctgcgcacgc ggcccggcga gagcggcgcg ctgcagcagg agctgctgat cagcgtgacc
     2401 aacttcttcc gcgacccgga gtgcttcgac gcgctcgcgc gcgagatccc ggcgatgctc
     2461 gaccgggccg agggcacgct gcgcgtctgg gtgccggcgt gcgcgacggg cgaggaagcc
     2521 tactcgatcg cgatgctgct ggccgagcac tcgcacgcgc tcgcgtcgcc gccggcggtg
     2581 cagatcttcg cgacggatct cgacgaggag gcgatccgca tcgcgcgcga cggcctctat
     2641 ccggacacca tcgcgaccga cgtgtcgcag gagcgcctgc gccgcttctt cgtgcgcgag
     2701 caccagggct ggcgcgtgcg ccgcgagctg cgcgagcgga tactgttcgc cgagcacgac
     2761 ctgctcaagg atccgccgtt ctcgcggctg gatctcgtgt cctgccgcaa cgtgctgatc
     2821 tacctgaacc gcgacggcca gcggcgggtg ctcgaggtcg cgcatttcgc gctgcggccg
     2881 caggggcggc tgttcctggg cgcgtccgag acggtggagg atcaccacgc gctgttctac
     2941 ccgctcgaca agaagcaccg cgtcttcgcc gcgcgccagt tgccgcgctc gcacctgccg
     3001 acgctggcgg ggccgggcat cctcgcccgc gcgctcgacc tgcacgagcc ggcgcgcgcg
     3061 tcgaacggcc tgccgcacgc ggccgcgcag gcggccgacg cggcgccgtc ggcgatcgat
     3121 gcacggccgg cgcgcggccc cggctcctgg gaaggccgtc ccgcctcgtg ggccgagctg
     3181 cacttcaaga tgctcgagat gttcgcgccg ccgtccatcg tcatcgacgg cgcgcacgag
     3241 atcatgcacg tctcggcgag cgccggccgg ttcctgcacc tcgggggcgg cgaacccacg
     3301 cgcaacctgc tgcagctgat ccacccgggc cttcgctcgg agctgcgcgc ggccatctac
     3361 aaggcgggcc acgccaccgg ccgcgcggag ctcgcgccca cgccgatcga gatcggcgga
     3421 caggtgctgc tggtgaaggc cgtggtcgcg gcgatggcgg acgtggcgcc gggcatgatg
     3481 ctcgtcacgc tggacgcgca gccggctggc gacgccgccg ggcttgcggc cgccgacggc
     3541 gcgcaggagg acggcgacgc gctcgccacg ctgctggagc gcgagctcga cggcctgaag
     3601 tcgcagctgc gctacaccgt cgagcagtac gaggcctcga ccgaggagct caaggccagc
     3661 aacgaggagc tgcaggccat gaacgaggag atgcgctccg cgaccgagga gctcgaaacg
     3721 agccgcgagg agctgcagtc gatcaacgag gagctcacga cggtgaacca cgacctcaag
     3781 ggcaaggtcg aggagctggg gcgcgccaac ggcgacctgc agaacctgat ggatgccacg
     3841 gcgatcgcca ccgtcttcct cgaccgcgag ctgtgcatcg cgcgctacac gccgacggcc
     3901 gtgagcctgt tcaatctcat ccccaccgac gtcgggcggc cgctgtcgca catgaccaac
     3961 cggctcgact acccgcagct cgaggccgac gcgcggcgcg cgctgcagga gctggtgccg
     4021 gtggagcgcg aggtcgccga cgcggatggc cgccgctacc tcgcgcgcac gcggccctgc
     4081 cgcacgggcg aggatcgcat ctcgggcgtg gtgctgacgc tgatcgacgt gaccgagcgc
     4141 aagcgcagcc aggaggcgct gcagctgtcc gaggagcgct tcggcgcgat cggagcggcg
     4201 ttgcgcgagc cgctcgccgc cgccgagcgg gccgccgcgg cgctggcgtc cccggacgcc
     4261 tgctcagacg cagaccgcgc gcaggcaccc gcgcttctgc agcggcagct ggcgaggatg
     4321 cgcggcctgc tggacgaact gctcgaccgc ccgcggcccg cgacctgacg acgcatcgct
     4381 tgaccgtcgc ctctgccggc cggcgacacc gcgatgcggg taaacccgta gtctcatcgc
     4441 ggacacctcg ggcgtaagct gacgcttgcg tgcatgcggg ccgcagcacg gcccgcgcgc
     4501 cccctcgccc cggaaggtag ctcatgcgcg atgtcgaccc ccagctgctg cgcttgtgag
     4561 aagtggaggc gcgcatcgcc ttcgccgagc gcgtgctgcg cgaccagcag gccatcgtgc
     4621 gccggctgac gagccagggc tattgcgccc agctcgccca cgagctggag gccaacatcc
     4681 gcgattcgct ggacatcctg gtgcggcgcc gcgaggacat cgtcgcggcg atgcaggcga
     4741 ccgacgcgcc gaccccctcg accccgcctg acacgctgct gcgccgctga cgtcgggcgg
     4801 ccgccggtcg atcgcgcggc gtccgcgcga ggtcgccgca tgcgtgcgcg gccc
//
LOCUS       DASVLN010000881         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_44257, whole genome shotgun sequence.
ACCESSION   DASVLN010000881 DASVLN010000000
VERSION     DASVLN010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44257"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..876
                     /gene="cysW"
                     /locus_tag="VF453_20735"
     CDS             4..876
                     /gene="cysW"
                     /locus_tag="VF453_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEX7690145.1"
                     /translation="MPARGAHPPLAGATAEPAWVRRSLVVAALAFLALFLFVPLGSVF
                     AQALAKGLDVYWAALVDPDALAALRLTLLAAGVSVPLNLVAGVAAAWAIAKFDFRGKS
                     LLLTLIDLPFSVSPVIAGLIYLLIFGLQGWFGAWLQAHDVRIVFAAPGIVLATVFVTF
                     PFVARELIPLMQAQGTEQEEAARVLGASGWQILWRVTLPNVRWALLYGVILCNARAMG
                     EFGAVSVVSGHVRGRTNTLPLHIEILYNDYQFAAAFAVASLLAALALVTLAAKAVVER
                     RAKSVEADFQGVAK"
     gene            986..>1967
                     /locus_tag="VF453_20740"
     CDS             986..>1967
                     /locus_tag="VF453_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HEX7690146.1"
                     /translation="MSIEIRNVTKRYGALAVCDDLSLDIPAGELVALLGPSGSGKTTL
                     LRIIAGLERPDAGRVLFHGEDGTGTEVRDRGVGFVFQHYALFGHMTIAENVAFGLRVR
                     PKATRPPEGEIRARVRQLLELVQLDWLADRHPHQLSGGQRQRVALARALAVEPKVLLL
                     DEPFGALDARVRKELRRWLRRLHDEMHVTSVFVTHDQEEAMEVADRVVVMHEGRVVQQ
                     GAPDEVYDRPATPFVLQFLGDVNLFRRPGGGDGEADYVRPHEIDIVAEPAADAWPVTL
                     GQTLTVGPNTRLEFQREDGGFVDVELTRQAFVEARARLGLAPGARVFLRPR"
BASE COUNT          230 a          699 c          732 g          306 t
ORIGIN      
        1 agcgtgcccg cgcgcggcgc gcatccgccg ctggccggcg cgaccgccga gcccgcctgg
       61 gtgcgccgtt cgctggtcgt ggccgcgctg gccttcctcg cgctgttcct gttcgtgccg
      121 ctcgggtcgg tgttcgcgca ggcgctggca aagggcctgg acgtctactg ggcggcgctc
      181 gtcgacccgg acgcgctggc cgcgctgcgg ctgacgctcc tggccgcggg cgtgtcggtg
      241 ccgctcaacc tcgtggccgg cgtggccgcg gcgtgggcga tcgcgaagtt cgacttccgt
      301 ggcaagagcc ttctgctcac gctgatcgac ctgccgttct ccgtctcgcc ggtgatcgcg
      361 gggctgatct acctgctgat cttcggcctg cagggctggt tcggcgcatg gctgcaggcc
      421 cacgacgtca ggatcgtgtt cgcggcgccc ggcatcgtgc tggcgaccgt cttcgtcacc
      481 ttccccttcg tcgcgcggga gctgatcccg ctcatgcaag cgcagggcac cgagcaggag
      541 gaggccgcgc gcgtgctcgg tgcctcgggc tggcagatcc tgtggcgcgt cacgctgccc
      601 aacgtgcgct gggcgctgct gtacggcgtg atcctgtgca acgcgcgggc gatgggcgag
      661 ttcggcgcgg tcagcgtcgt ctcgggccat gtgcgcgggc gcacgaacac gctgccgttg
      721 cacatcgaga tcctctacaa cgactaccag ttcgcggccg cgttcgccgt ggcctcgctg
      781 ctggcggcgc tggcgctcgt cacgctggcg gccaaggccg tcgtcgaacg gcgcgcgaag
      841 tccgtcgagg ccgacttcca aggagttgcg aaatgacctg cttcccagcc gtgcgctctc
      901 gggcggagcg ccgggccgcc ccaagccggc ccgctgttcc ctcgggggag cggccgacgg
      961 ttccgtcgac cgaggggctc gtggcatgag catcgagatc cgcaacgtca ccaagcgcta
     1021 cggcgcgctc gcggtctgcg acgacctgag cctggacatc cccgccggcg agctcgtcgc
     1081 gctgctcggg ccttcgggct cgggcaagac gacgctgctg cgcatcatcg cgggcctcga
     1141 gcgcccggac gcgggccgcg tgctgttcca cggcgaggac gggaccggca ccgaggtgcg
     1201 cgatcgcggc gtcggcttcg tgttccagca ctatgccctc ttcggccaca tgacgatcgc
     1261 cgagaacgtc gcgttcggcc tgcgcgtgcg gccgaaggcg acgcgtccgc cggaaggcga
     1321 gatccgcgcg cgcgtgcggc agctgctcga gctcgtccag ctggactggc tggccgaccg
     1381 ccatccgcac cagctctcgg gcggccagcg ccagcgcgtg gccctcgcgc gggcgctggc
     1441 ggtcgagccc aaggtgctgc tgctggacga acccttcggc gcgctcgacg cgcgcgtgcg
     1501 caaggagctg cggcgctggc tgcgccggct gcatgacgag atgcacgtca cgagcgtctt
     1561 cgtcacgcac gatcaggagg aagcgatgga ggtcgccgac cgcgtcgtcg tgatgcacga
     1621 gggccgggtg gtgcagcagg gcgcgccgga cgaggtctat gaccgcccgg ccacgccctt
     1681 cgtgctgcag ttcctcggcg acgtgaacct gttcaggcgc ccgggcgggg gcgacggcga
     1741 ggccgactac gtgcgcccgc acgagatcga catcgtcgcc gaacccgccg ccgacgcctg
     1801 gccggtgacg ctgggccaga cgctcacggt cgggccgaac acgcgcctgg agttccagcg
     1861 cgaggacggc ggcttcgtcg acgtcgagct cacgcgccag gccttcgtcg aggcgcgggc
     1921 gcggctgggg ctcgcgccgg gggcgcgcgt gttcctgcgg ccgcggc
//
LOCUS       DASVLN010000882         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_157349, whole genome shotgun sequence.
ACCESSION   DASVLN010000882 DASVLN010000000
VERSION     DASVLN010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157349"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /gene="iolB"
                     /locus_tag="VF453_20745"
     CDS             <1..504
                     /gene="iolB"
                     /locus_tag="VF453_20745"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEX7690147.1"
                     /translation="AEVALCTAPAGDAPRDVRRIDPRTMRRSVRGQGSNQRFVCDILP
                     HDDPTAAHLLVVEVRTPAGHSSSYPPHKHDTEAPPGETQLEETYYHRVHPPQGFAFQR
                     VYTDDRSLDEACAVEDRDLVLVPRGYHPVVAPHGYQLYYLNVMAGPHRFWVFRNDPAH
                     EWMLKPA"
     gene            complement(523..>1572)
                     /locus_tag="VF453_20750"
     CDS             complement(523..>1572)
                     /locus_tag="VF453_20750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7690148.1"
                     /translation="SARRFDLGGEAATGGAAAAPADTGAVLLHVVTRRYEHDGRPYFV
                     QVAVSERAAELVGEVIVGPVLLKNAAWISVVALVLFVIGVHMTLDYLLRPLREGSRAA
                     ARIDPRNLAARLDVGCMPRELLPLITAFNAALDRLETGYRAQQEFLAAAAHELKTPLA
                     LIRGQVELSDSCERETLLRDIDLMARQVHQLLHLAEASEVTNYSFEPLDVGAVAAEAA
                     DLLQRLAQRAGVHVDVRLPGEPVVRRADRSAVFVLAKNLVENAIQHSPRGAVVSVCVA
                     PDGMTVRDEGAGIPADHLDRLFTRFWRGPHRRDSGAGLGLAICAEVAIAHDWRLAAAN
                     GDAGGAVFSVAFDAR"
BASE COUNT          217 a          606 c          559 g          190 t
ORIGIN      
        1 gccgaggtcg cgctgtgcac cgcgcccgcc ggcgacgcgc cgcgcgacgt gcgccggatc
       61 gacccgcgca ccatgcgccg cagcgtccgc ggccagggca gcaaccaacg cttcgtgtgc
      121 gacatcctgc cgcacgacga cccgaccgcg gcgcacctgc tggtggtcga ggtgcgcacg
      181 cccgcgggcc attcgtcgag ctacccgccg cacaagcacg acaccgaggc gccgcccggc
      241 gagacccagc tcgaggagac ctactaccac cgcgtccacc cgccgcaggg cttcgcgttc
      301 cagcgcgtgt acaccgacga ccgcagcctc gacgaggcct gcgccgtcga ggaccgcgac
      361 ctcgtgctgg tgccgcgcgg ctaccatccg gtcgtcgcgc cgcacggcta ccagctgtac
      421 tacctcaacg tgatggccgg gccgcaccgg ttctgggtgt ttcgcaacga cccggcgcac
      481 gagtggatgc tcaagccggc ctgaccggcc gcgccgagga cttcaacgcg cgtcgaacgc
      541 gacgctgaac accgcgccgc cggcgtcgcc gttggcggcc gcgaggcgcc agtcatgggc
      601 gatcgcgacc tcggcgcaga tcgcgaggcc cagccccgcg ccgctgtcgc gccggtgcgg
      661 gccgcgccag aagcgggtga acagcctgtc aaggtggtcg gccggaatgc cggcgccctc
      721 gtcgcgcacc gtcatgccgt cgggcgcgac gcacacgctg accaccgcgc cgcgcggcga
      781 gtgctggatc gcgttctcga ccaggttctt ggccagcacg aagacggcgc tgcggtcggc
      841 gcggcgcacg accggctcgc ccggcagccg cacgtcgacg tgcacccccg cgcgctgcgc
      901 gagccgctgc agcaggtcgg ccgcctcggc ggcgacggcg ccgacgtcca acggctcgaa
      961 gctgtagttg gtcacttcgc tcgcctcggc caggtgcagc agctggtgca cctggcgcgc
     1021 catcaggtcg atgtcgcgca gcagggtctc gcgctcgcag gagtcgctga gctcgacctg
     1081 gccgcggatc agcgcgagcg gcgtcttcag ctcgtgggcg gcggcggcga ggaactcctg
     1141 ctgcgcgcgg tagccggtct cgaggcggtc gagcgccgcg ttgaacgccg tgatcagcgg
     1201 cagcagctcg cgcggcatgc agccgacgtc cagccgcgcc gcgaggttgc gcgggtcgat
     1261 gcgcgcggcc gcgcgcgagc cctcgcgcag cgggcgcagc aggtagtcga gcgtcatgtg
     1321 cacgccgatg acgaacagca ccagcgcgac gaccgagatc caggccgcgt tcttcagcag
     1381 caccgggccg acgatcacct cgcccaccag ttccgcggcg cgctcgctga ccgccacctg
     1441 cacgaagtac ggccggccgt cgtgctcgta gcgccgcgtg acgacgtgca gcagcaccgc
     1501 gccggtgtcc gccggcgcgg ctgcggcgcc cccggtggcc gcctcgccgc cgaggtcgaa
     1561 tcgccgcgcc ga
//
LOCUS       DASVLN010000883         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_531498, whole genome shotgun sequence.
ACCESSION   DASVLN010000883 DASVLN010000000
VERSION     DASVLN010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531498"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VF453_20755"
     CDS             <1..718
                     /locus_tag="VF453_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7690149.1"
                     /translation="GLVLYFLHYLLSNAANYVFPIFAERGLGVPLATTGLLDTFAAVV
                     SFAGAWVYVKTASRLPRKKPLMAAGALLLAGAAWAFSALPAGAPAAMLLPALVAKGLF
                     GVLLVLPVAGLTFRDLGDERFAHGYQSKNLMRQIAGSFATALAAVALQDREFADRDAV
                     AATLDPSRPDVQRWLDQVQAGFAAHGLAAGQAHAAALATLARTVDQQVQLIACQDVYR
                     GLAVFAVAAAVVVVAQRRLK"
     gene            770..1318
                     /locus_tag="VF453_20760"
     CDS             770..1318
                     /locus_tag="VF453_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7690150.1"
                     /translation="MPRRRTPPSPDAPTAPAVADDPAGQDPLLLRRLLRAKDRMDAAS
                     HEAWPVPRLAEVSGVSQAHFARSFKQAFGVPPHRYLLTRRIERATALLRETELPITEV
                     AFQTGWESLGTFGRTFRDITGESPGAVRARERAAARDPAPVPHCILSAAHRPDLTMAV
                     SEKRRREDAGTNASPIQPKEIP"
     gene            1315..1722
                     /locus_tag="VF453_20765"
     CDS             1315..1722
                     /locus_tag="VF453_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7690151.1"
                     /translation="MTAGIETVGLYVRDQDEALDFYVNKLGFQVHTDVRNGPYRWLTV
                     QHPQQPGFQLGLYVPGPPTHDAATAQSLAALVAKGAMPPLVLRVDDCRAAYDRLLGLG
                     VEFTQEPIARFGTVDAGFRDPAGNGWKMIEARR"
     gene            1719..1979
                     /locus_tag="VF453_20770"
     CDS             1719..1979
                     /locus_tag="VF453_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690152.1"
                     /translation="MNARAPALRAAAADAPPARSGADAWIRPLHRWMAIVFSAAAIAN
                     IAALVLRVSAPWLGLLAVVPLLPMMATGLWMFVQPYRAKRRQ"
     gene            2045..>2240
                     /locus_tag="VF453_20775"
     CDS             2045..>2240
                     /locus_tag="VF453_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006024792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7690153.1"
                     /translation="MDAPTLAAYARSAADYAADWLAQPAPEDLYALLRTHFRPGPTLD
                     VGCGAGRDTAWLAANGFEARG"
BASE COUNT          269 a          894 c          780 g          297 t
ORIGIN      
        1 gggcctggtg ctgtacttcc tgcactacct gctgtcgaac gcggccaact acgtcttccc
       61 gatcttcgcc gagcgcggcc tgggcgtgcc gctcgcgacg accggcctgc tcgacacctt
      121 cgctgccgtc gtcagcttcg cgggcgcctg ggtctacgtg aagacggcgt cgaggctgcc
      181 gcgcaagaag ccgctgatgg cggccggcgc gctgctgctc gcgggcgcgg cctgggcctt
      241 ctcggcgctg ccggccggcg cgccggccgc gatgctgctg ccggcgctcg tcgccaaggg
      301 cctgttcggc gtgctgctgg tgctgccggt cgcgggcctg acgttccgcg acctcggcga
      361 cgagcgcttc gcccacggct accagagcaa gaacctgatg cgccagatcg ccggctcgtt
      421 cgcgaccgcg ctcgcggccg tcgcgctgca ggaccgcgag ttcgccgacc gggacgcggt
      481 cgccgcgacc ctggatccgt cccgccccga cgtgcaacgc tggctcgacc aggtgcaggc
      541 cggcttcgcc gcgcacggcc tagcggccgg ccaggcccac gcggccgcgc tcgcgacgct
      601 ggcccgcacc gtcgaccagc aggtgcagct gatcgcgtgc caggacgtct accgggggct
      661 ggcggtgttc gcggtggcgg ctgcggtggt ggtcgtcgcg cagcggcgcc tgaagtgagc
      721 ccggcggcgg cagagtggcc gagaatccgc ccaggagact cccccgccaa tgccccgccg
      781 ccgcacgccg cccagccccg acgcgcccac cgctccagcc gtggcggacg atcccgccgg
      841 ccaggatccc ctgctcctgc gccgcctgct gcgcgccaag gaccgcatgg acgccgcgtc
      901 ccacgaagcc tggccggtgc cgcggctggc ggaggtcagc ggcgtctcgc aggcgcactt
      961 cgcgcgctcg ttcaagcagg ccttcggcgt gccgccgcac cgctacctgc tgacgcgccg
     1021 catcgagcgc gcgaccgcgc tgctgcgcga gaccgagctg ccgatcaccg aggtcgcgtt
     1081 ccagaccggc tgggagagcc tgggcacgtt cggccgcacc ttccgcgaca tcaccggcga
     1141 gagcccgggc gccgtgcgcg cgcgcgagcg cgccgccgcg cgcgatccgg cgccggtgcc
     1201 gcactgcatc ctcagcgccg cgcaccggcc cgacctcacg atggcagttt cggagaagcg
     1261 ccgccgcgag gacgccggta caaacgcgtc accgatccaa cccaaggaga ttccgtgacc
     1321 gcaggcatcg aaaccgtcgg actctacgtc cgcgaccagg acgaggcgct cgacttctac
     1381 gtcaacaagc tcggcttcca ggtgcacacg gacgtgcgca acggcccgta ccgctggctg
     1441 accgtccagc atccgcagca gcccggcttc cagctcggcc tgtacgtgcc gggcccgccg
     1501 acgcacgacg ccgcgaccgc gcagtcgctg gccgcgctgg tggccaaggg cgcgatgccg
     1561 ccgctcgtgc tgcgcgtcga cgactgccgc gccgcctacg accgcctgct cgggctgggc
     1621 gtcgagttca cgcaggagcc catcgcccgc ttcggcaccg tcgacgccgg cttccgcgat
     1681 cccgccggca acggctggaa gatgatcgag gcccgtcgat gaacgcccgc gcccccgccc
     1741 tgagggccgc cgccgccgac gcgccgcccg cgcgcagcgg tgccgacgcc tggatccgcc
     1801 ccctgcaccg ctggatggcg atcgtgttct cggctgcggc catcgccaac atcgcggcgc
     1861 tcgtgctgcg cgtgtccgcg ccctggctcg ggttgctggc ggtcgtgccg ctgcttccga
     1921 tgatggccac ggggctgtgg atgttcgtgc agccctaccg ggcgaagcgg cggcagtgac
     1981 cgccgccgcc ggcctcgacc ggactcgtgc gatgctccgg gccttcgcac ctccgaccgc
     2041 cgccatggac gccccgaccc tcgccgccta cgcccgatcc gccgccgact acgccgccga
     2101 ctggctcgcc cagcccgcgc cggaggacct gtacgcgctg ctgcgcacgc acttccgccc
     2161 ggggccgacg ctggacgtcg gctgcggcgc cgggcgcgac accgcctggc tggcggccaa
     2221 cggcttcgag gcccgcggcg
//
LOCUS       DASVLN010000884         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_44445, whole genome shotgun sequence.
ACCESSION   DASVLN010000884 DASVLN010000000
VERSION     DASVLN010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44445"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..2007
                     /locus_tag="VF453_20780"
     CDS             229..2007
                     /locus_tag="VF453_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX7690154.1"
                     /translation="MDRRQFLRRGAFFTVVAASPIALTACGGDATAAAGTYQFTEGVA
                     SGDPRDTSAVFWTRVTRVDGTDEAIDVRLEVSTAADFSAIVGQADLQASATYDHTVRA
                     KVTGLPPATPLFYRFRAGADTSAVGNAKTAPAAGASVAQMNFAWFTCQDWSVNHWGAM
                     SLLGQESLDFVVHVGDYIYETVGAAFQAGAAEPAHTQLAFPDGTQLGTSTFYATTLAD
                     YRYLYRTYRGDARLQAIHRQWPMVAIWDDHEFSDDCWQDHQTYTNENVQQTARRRAAS
                     QAWIEYMPIDFGDVSFDTANQAYDNIRIYRDFRFGDLMHLVMTDERLYRDDHVVLESA
                     VAAAQGHDPINGSDSIGSRYFVQQPVLAQFEAAKTASLGRAPSVLGVGQAQWWKQTMS
                     GSTATWKVWGNEVMLNRLWVDLRQLAPPPYNAVYVVNCDSWDGYPSHKADLLGYLKAN
                     NVTNVVAITGDLHAFQAGVVRDVPDPATGTPVLVDFVTAGISSTSLFKYLQAGAAGTP
                     LASLVANQPTFEAIVGANNPDLTYHDHDAQGYATASVTPGSFVVTYTKVKPLNADGTA
                     PASPLLKRTRITVNAGSTTPVVQDDV"
     gene            2071..3066
                     /locus_tag="VF453_20785"
     CDS             2071..3066
                     /locus_tag="VF453_20785"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7690155.1"
                     /translation="MNQIPPSDPYAPAPRRGGRFHNASGRAQQGLGGVLRWLATRKRG
                     AWPSKVEDVPVPPPPARVDDGAIHATPVGHATVLVQMDGLNLLTDPVLSHRIGPTSWL
                     GPTRVRPPAIPFAELPRIDVVLLSHDHYDHLDRPALRALAARDDPLVVTGLGVGEKVP
                     SRRVVELDWWRAHEVDARVRVTYVPAEHFSGRGPFDRDKRLWGGFVVEGPSGRLYFAG
                     DTAAGWHFGAIRQRFGPMTLSLIPIGAYLPRWFMAPVHIDPPEALDASITLASQVSLA
                     IHYGTFDLADDGYDAPPQALARALAALEGRPAGSDFRVVPFGETAVVRPGDAAAR"
BASE COUNT          450 a         1203 c         1020 g          404 t
ORIGIN      
        1 tgctcgcggc gcacacgagg gcggccttgc cgcgcagtcc gaagtccatg gcgccggagt
       61 ctcgcggccg gcggcggggc ggtcaaggcc cgcggcggca tcggggatgt cccgcaggct
      121 gcggcatcgt cgcaacagcc gcgcacgtct ggggagaagc ccccagaacg cgtcatgacg
      181 gcgtcgctat cctcgcgccc actacaaggt ttcggagact tccccaccat ggatcgcaga
      241 cagttccttc gccgcggcgc cttcttcacc gtcgtcgcgg ccagcccgat cgccctgacg
      301 gcctgcggcg gcgacgccac ggccgccgcc ggcacctacc agttcacgga aggcgtggcg
      361 agcggcgacc cgcgcgacac cagcgccgtc ttctggaccc gcgtcacccg cgtggacggc
      421 accgacgagg ccatagacgt ccgcctcgag gtctcgaccg cggccgactt ctcggccatc
      481 gtcggccagg ccgacctgca ggcgagcgcg acctacgacc atacggtgcg cgccaaggtc
      541 accggcctgc cgccggccac gccgctgttc taccgcttcc gcgccggcgc cgacacgagc
      601 gcggtcggca acgcgaagac cgccccggcc gccggcgcga gcgtcgcgca gatgaacttc
      661 gcgtggttca cctgccagga ctggtcggtc aaccactggg gcgcgatgtc gctgctcggg
      721 caggagtcgc tcgacttcgt cgtccacgtc ggcgactaca tctacgagac ggtcggcgcc
      781 gcgttccagg ccggcgccgc cgagcccgcg cacacgcagc tcgccttccc cgacggcacc
      841 cagctcggca cctcgacgtt ctacgcgacg acgctggccg actaccgcta cctgtaccgc
      901 acctaccgcg gcgacgcgcg cctgcaggcg atccaccgcc agtggccgat ggtcgcgatc
      961 tgggacgacc acgagttcag cgacgactgc tggcaggacc accagaccta caccaacgag
     1021 aacgtgcagc agaccgcgcg ccgccgcgcc gcgagccagg cgtggatcga atacatgccg
     1081 atcgacttcg gcgacgtctc gttcgacacc gccaaccagg cctacgacaa catccgcatc
     1141 taccgcgact tccggttcgg cgacctgatg cacctcgtga tgaccgacga gcgcctgtac
     1201 cgcgacgacc acgtcgtgct cgagtcggcc gtcgccgccg cgcagggcca cgacccgatc
     1261 aacggcagcg actcgatcgg ctcgcgctac ttcgtgcagc agccggtgct cgcgcagttc
     1321 gaggcggcca agacggcctc gctcggccgc gcgccctcgg tgctgggcgt cgggcaggcc
     1381 cagtggtgga agcagacgat gtcgggctcg accgcgacct ggaaggtctg gggcaacgag
     1441 gtcatgctca accgcctctg ggtcgacctg cgccagctcg cgccgccgcc gtacaacgcg
     1501 gtctacgtcg tcaactgcga ctcgtgggac ggctacccga gccacaaggc cgacctgctg
     1561 ggctacctga aggcgaacaa cgtcaccaac gtcgtcgcga tcacgggcga cctgcacgcg
     1621 ttccaggcgg gcgtcgtgcg cgacgtgccg gatcccgcga ccggtacccc ggtgctggtg
     1681 gacttcgtca cggccggcat ctccagcacc agcctgttca agtacctcca ggccggcgcg
     1741 gccggcacgc cgctcgcgtc gctggtagcc aaccagccga ccttcgaggc catcgtgggc
     1801 gccaacaacc ccgacctcac gtaccacgac cacgacgccc agggctacgc cacggccagc
     1861 gtcacgccgg gctcgttcgt cgtgacctac accaaggtca agccgctgaa cgccgacggc
     1921 acggcgccgg cgtcgccgct gctcaagcgc acgcgcatca cggtgaacgc cggcagcacg
     1981 acgccggtcg tgcaggatga cgtctgagtt ccgggcgggc ccgagaccgg acggcgtaca
     2041 gtcgcccgga aacgcttcca cgcccgccac gtgaaccaga tcccaccgtc cgacccctac
     2101 gcccccgcgc cccgccgcgg cggccgtttc cacaacgcga gcggccgcgc gcagcagggc
     2161 ctcggcggcg tgctgcgctg gctcgcgacg cgcaagcgcg gcgcatggcc gtccaaggtc
     2221 gaggacgtcc ccgtcccgcc gccgcccgcg cgcgtggacg acggggcgat ccacgcgacg
     2281 cccgtcggcc atgccaccgt cctcgtgcag atggacggcc tgaacctgct caccgacccg
     2341 gtgctgtcgc accgcatcgg accgacctcg tggctcggcc cgacgcgcgt gcgcccgccc
     2401 gcgatcccgt tcgccgagct gccgaggatc gacgtcgtgc tcctgagcca cgaccactac
     2461 gaccacctgg accgcccggc cctgcgcgcg ctcgcggcgc gcgacgatcc gctcgtggtc
     2521 accggcctgg gcgtcggcga gaaggtgccg tcgcgacgcg tcgtcgagct cgactggtgg
     2581 cgcgcccacg aggtcgacgc ccgcgtgcgc gtcacctacg tgccggcgga gcacttctcc
     2641 ggccgcggcc cgttcgaccg cgacaaacgg ctgtggggcg gcttcgtcgt cgaagggccg
     2701 tcggggcgcc tgtacttcgc gggcgacacg gcggcgggct ggcacttcgg cgccatccgc
     2761 cagcgcttcg ggccgatgac gctgtccctg atcccgatcg gcgcctacct gccgcgctgg
     2821 ttcatggcgc ccgtgcacat cgatccgccg gaggcgctgg acgcgagcat caccctcgcc
     2881 tcgcaggtct cgctcgcgat ccactacggc accttcgacc tcgccgacga cggctacgac
     2941 gccccgccgc aggcgctcgc gcgcgcactg gcggcgctcg agggacgtcc cgccggcagc
     3001 gacttccgcg tcgtgccgtt cggcgagacc gcggtcgtgc gcccgggcga cgcggcggcc
     3061 cgctgatcgg ccgcggg
//
LOCUS       DASVLN010000885        20326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_422594, whole genome shotgun sequence.
ACCESSION   DASVLN010000885 DASVLN010000000
VERSION     DASVLN010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 20326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20326
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422594"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VF453_20790"
     CDS             complement(<1..220)
                     /locus_tag="VF453_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7690156.1"
                     /translation="MTASPSLPNASDARPPMQVATFHSLRGRRVFVTGGGSGIGAALV
                     EAFAAQGAHVAFVDIAAEASAALARAIES"
     gene            392..2113
                     /gene="araD"
                     /locus_tag="VF453_20795"
     CDS             392..2113
                     /gene="araD"
                     /locus_tag="VF453_20795"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEX7690157.1"
                     /translation="METKKPVRRSQAWFGKNDRDGFVHRSWIKNQGYPHDELDGRPVI
                     GICNTWSELTPCNGHFRELAEFVKRGVYEAGGFPLEFPVMSLGETQLRPTAMLFRNLA
                     SMDVEESIRGNPIDGVVLLMGCDKTTPALMMGAASCNLPTIGLSGGPMLNGKFRGQDI
                     GSGTGVWKMSEMVRAGEMTQADFTAAESCMHRSKGSCMTMGTASTMASMVESLGMSLP
                     ENAAIPAADTRRNRLAQLTGRRIVEMVKEDLTMSKILTRTAFENAIRTNAAIGGSTNA
                     VIHLLAIAGRMGVDLKLADWDKLGAEVPCLVDLQPSGRFLMEDFFYAGGLPAVLREIS
                     HLLKLDAPTVNGKTLGENIADAPCWNREVIRPLDKPLVANAGIAVLRGNLAPDGAVIK
                     PSAASPHLLKHRGRAVVFETIEEFHARIDDEDLDIDEHCVMVLKNCGPKGYPGMAEVG
                     NMPLPPKVLRKGITDMVRISDARMSGTAYGTVVLHTSPEAAAGGPLALVENGDMIELD
                     VPARSLKLEVSDEELAARRARWQPLPRPERGWQKLYVDHVQQAHLGADLDFLVGSSGA
                     GIPRDSH"
     gene            2148..3047
                     /locus_tag="VF453_20800"
     CDS             2148..3047
                     /locus_tag="VF453_20800"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7690158.1"
                     /translation="MAGRPQQLSQPRVLSDVRATLGEGLCWSPRAQAIWSVDILRHRL
                     YRDTLAGEHLEWSFDETVSAVAERAQHPGLAIALRRGLALFDPATGQTTRLHEPEPER
                     TGNRFNDGKCDARGRFWGTTMDYAVQAAAGAYYRYDAAGRAERVLDCGWVVTNGPTWS
                     RDGRTIFLNETAQRRVHAAAFDMETGAIGPLREFLRLSEPDGHPDGMTTDAEGRLWIA
                     HWGGACVTCHDPDTAEELARIALPTDHVSNVCFGGPDLRTLFVTTARFELSEAQLAAQ
                     PLAGALFAVEVDAQGVAPNLYAG"
     gene            3108..4067
                     /locus_tag="VF453_20805"
     CDS             3108..4067
                     /locus_tag="VF453_20805"
                     /inference="COORDINATES: protein motif:HMM:NF040027.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6216 family protein"
                     /protein_id="HEX7690159.1"
                     /translation="MNPINLPEIARTAGDWFGVQGSVLYAVALAIALGWFCWRTRSIH
                     PVTGWMWRVFVGKDDFQDPDLKRFLDTRQKLLKFRFVTGIPARTWDKAKDIIAWGEDN
                     GEELGDIAACGAHFDKERCELKGKRRGLLWQMAGWLLLQVLFVVLLTALFALHRIDRA
                     VLSMKTSGTWIAASADQVKRISGNEAVTAQDCKPDAGRVAARLSVTPDEAKAICNAFS
                     DRKFPKFIEDNVAEQRGLAARGASFFLFLAALGLLGIRHAGAAIDMRQRLDRKNGAAR
                     AGGAEADVRVELPALQITVQAELKSAAPTPRRRSRPKAPGTLA"
     gene            4465..5640
                     /locus_tag="VF453_20810"
     CDS             4465..5640
                     /locus_tag="VF453_20810"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7690160.1"
                     /translation="MLLLVVTAPVALASGVFALQILLGSSKRSGKIDAIPVRPTGAIA
                     VVVPAHDEERGIARTLLSIRAQLRSGDRLVVVADNCADATASVARDHGATVIERTHAT
                     LRGKGFALAAGIDALRGDPPAIVVLVDADCELAPGAVDALARATTHAGRPSQARYLML
                     HPEGASLPKRLAQFAWRVRNWARPAGWHRIGAPCQLMGSGMCFRWETLRDAPIANDSI
                     VEDMRLGIDLALMGQPPVFCPEALVTSMFPDSAAASKTQRMRWEHGHLEMIARSALPL
                     LWQGLRRRDKHLVLMALDLLVPPLALLGALLTSLFLASLGLAWITHATEAAWMNGALV
                     ALFLAAALQSRRAYGRDLVDLVELAAIPAYIARKLPMYGRFVTKRQRGWVRTDRDVQ"
     gene            6117..6680
                     /locus_tag="VF453_20815"
                     /pseudo
     CDS             6117..6680
                     /locus_tag="VF453_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023098450.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7064..7495)
                     /locus_tag="VF453_20820"
     CDS             complement(7064..7495)
                     /locus_tag="VF453_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7690161.1"
                     /translation="MHIERHEIGPRFSEMTVVDVGTGKLVFIAGQVAENTALDIGGQT
                     REVLGFIDRLLTHVGARREHIVSARVYLASVGDYAQMNAVWDDWVVPGHTPTRATIGA
                     KLINSDYKVEIEVTAAFTPHADGDHAHDDGCEKAPSVHDHR"
     gene            complement(7536..8996)
                     /locus_tag="VF453_20825"
     CDS             complement(7536..8996)
                     /locus_tag="VF453_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7690162.1"
                     /translation="MSKLHFCHRIGLTIGSVAWLAGCAVGPDYHRPESPAAAYLPAPV
                     EAAMRDERQVVDADLPAQWWTLFHSPALDELVRGALARSPSVAAAQAALRQATELRRA
                     QQASFFPTVDASYSASRQKVARPLASPLASNADLFNLSTAQLSVSYTPDLFGMNRRQV
                     ESLQAQEQAQRWNVEATYLTLSSNLVVAVITEASLHAQFEATTRQIALQSEVLARFRE
                     LERLGENSELDVAQQESQLAALQATLPGVYKQMVQQRDLVKALAGLPPTADVPEPTLD
                     DLTLPDPLPLTLPSTLVEHRPDVLAAEAQLQSASADIGVAVANRLPQVTLGVNTWGSS
                     AASLSGLFAPGTSFWTLAGGVTQPLFDGGALKHKEDAARAAYDQAEATYRATVINAFQ
                     NVADALQAVQADAEAERATWHALDLADRTLAIARRQLELGDISALAVSQSEQARQQAL
                     VGYVQARAQRLQDAAALLQSLGGGWWNREHAQAARD"
     gene            complement(8996..12091)
                     /locus_tag="VF453_20830"
     CDS             complement(8996..12091)
                     /locus_tag="VF453_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX7690163.1"
                     /translation="MINQFIALCFRRRLLVWVLTLLILVYGWFSWTQMTVEAYPDISD
                     VTVQVTTQVPGLAAEEIEQQITTPLERALANTPGLVTMRSSSTFALSLITLVFKDGTD
                     DYFARQRVTERLAQVTMPAGVQPGLDPISSPVGEIYRYTLESDTKNLMQLSEIQRWVV
                     MPAFRQVPGIADVNNFGGFTKEFEIELDPRQLDRYGLALNDVVTAINNGSQNSGGGRI
                     SRGEQSYVVRGIGQAHSLDDLGSIAVATSSNVPVQLRELGKLQIGHQEREGILGKDSD
                     PDTIEGIVLLLKGQNPSKVLEGVHAKVDELRKRLTPLGVKIVPYIDRDQLVKLTVEKV
                     MHTVLEGVGLVCVVLILFLGSPRSAIVAAVAIPLALVTVFIAMNLLHMPANLFSLGAI
                     DFGIIVDGAIVVTEAMLRRREEQPDQPLVEDDVLETTGQIARPIFFATLIIVCAYFPL
                     FAFERAEGKLFSPMAFTVGFALFGALLGCMALIPGLAFVALRKPRRVFHNRPLIWLTD
                     RYRNALGWLLGRPPVIYLVSLLVLAAVGVLGATAGREFLPELDEGALWLQVQMPTGLS
                     LDKASEMASELRRTLREFPEISYVVTQLGRNDDGTDPWTPSHMEVPVGLKPYAEWPDH
                     ESKAEFVRRLNDKLQRIPGFDVGISQPIIDGVNDAIGGAHSPLVMRVYGQDIADDRRI
                     ASQVVDILHTVRGTASASIFQEPQIPQLVVKVDREAAGRVGIPVSDISALIQTGIGGA
                     AVTTLYQEDRQYNVTVKFPRSAKGSLQDLGALTLTAPGGARIPLSQLASIQYKTGEST
                     ISHEMNERQITVRVDNRDRDLTSYLAEAQRRIDAEVKFDPTKHHFEWAGQFENERRAQ
                     ARLVFILGLVFAMMAVLLFFQFGKVRQTLLVLGVVPMATLGGLIALRITGETLNVATA
                     VGFIALFGVSVQNGIIMVANFRRVRGEGYALLESVVRAATERLRPVLMTATVASIGML
                     PAALATGVGTDVQRGVATVVVSGLVVSTLLTLLVLPVLYFAIERHFESRERDQKMRKT
                     R"
     gene            complement(12088..13122)
                     /locus_tag="VF453_20835"
     CDS             complement(12088..13122)
                     /locus_tag="VF453_20835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7690164.1"
                     /translation="MAEPGPMFVEQNGRIVVPADSPLRQRLAVAEVTTRPGARRLSVP
                     AVVEADPSRVVAIVAPLTGRLVELHVGLGDSVHRGQPLATISSPDFGQALDDAAKAAD
                     ALELATRALERAKGVQAVGANAGKDLEAAELAQRDAAAEDRRARDRVSTLAGGTRVDT
                     RTHALVVLAPVAGTVTALNVGAGTFVNDATASLMTVSGLDRVFVTAQLPEQQAAAVAP
                     GTPVDVTLAGAVGQVWHGKAARVSPVLEPDTRRAKVRIPFDNADGRLRPNAFATATFA
                     LPEADGVSVPPSALLMNNDSTTVLVEVAPWTFVRRVVEVGAEDEQSVHILAGLKRGER
                     AVVRGGVLLQ"
     gene            13346..13804
                     /gene="cadR"
                     /locus_tag="VF453_20840"
     CDS             13346..13804
                     /gene="cadR"
                     /locus_tag="VF453_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HEX7690165.1"
                     /translation="MKIGDLATSTDTPVETIRYYEREGLMSAPARSSGNFRLYDEGHV
                     RRLGFIRACRALDMNLGEIRVLLRFRDGGESDCGEVNALLDEHLEHVEERMRELRRLA
                     KDLRALRDECRKASPTDQCGILRGLDRGAFEAPAKTKSHVGHVHRKGGKS"
     gene            complement(13815..15158)
                     /locus_tag="VF453_20845"
     CDS             complement(13815..15158)
                     /locus_tag="VF453_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7690166.1"
                     /translation="MTDMPTATAAPAVPVAELCAVSKSYKTAGGHDLKVLENIDLAVR
                     PGELLALLGQSGSGKSTILRCLTGLVEPTSGRALRNGKLLQGINPDVSIVFQTFALYP
                     WLTVEQNVAVGLMSRKMDRAEREAAVDHAIDLIGLGNYHAAYPRELSGGMRQRVGIAR
                     ALVSQPKMLCLDEAFSALDVLTAENLRQELIALWRSKENGISSIFMVTHNIEEAVEMA
                     TRIVVVFPRPGRLGLVLDNPLPYPRDPKSAGFQRLVGIIHETITTLTLPDLPPEAPPA
                     PGQAVSRARSRMESIPQVPVAQILGLLEILDGAPELKNIYEISDDIGKEFGETISIVK
                     AAEILDFVDTPKHDVELTELGKRFIASDREGRRKIFAEQVYKLRIFHIIIALLGEYEE
                     IEAERVIKDVASALPYDNPEKTFETMIAWGRYAGLMDLNPKTNKVFVPKDEDAPG"
     gene            complement(15161..16906)
                     /locus_tag="VF453_20850"
     CDS             complement(15161..16906)
                     /locus_tag="VF453_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7690167.1"
                     /translation="MKRSIAPQLQVAEPRRGFTWVDGVVMLALLAVFWSALHFGKGML
                     VHFDAASQPAIDLSVSMIPYYAGRTLLRMWIAFAFSLLFAVGTGYLAAKSARARMFIL
                     PALDVLQSVPVLGFLSATVTGFMALFPGSLMGVECAAIFAIFTGQVWNMAFGFYHSMV
                     TIPQDMQEAATTYGLSRWQRFTTVELPASAHSLIWNSMMSFGGGWFFVAQSEAITVMN
                     KDIKLPGLGSYMAQAIAAGSNNAALWAVVAMIVLILVSDQLVWRPLLAWADKFKIELT
                     VSQMPATSWVYDLLRGAYVFTWLGEHIVRPLAEHGASIGRRQARRLASVRVVRADHVW
                     RVVVVVAVAWLAYEMIEGAVAAAEELRGALSWKLVAHIAWLGVLTMLRVGAMTVLATL
                     VWTPVGVWIGFQPKVARFAQPLAQIGASFPVNMTFPVVVGWFVATHISINWGSILLIA
                     MGTQWYILFNVVAGAMAIPNDLKEAARVYGLRRWDLWRTLILPAIFPFWVTGACTAAG
                     GAWNASIVAELATFGDTTLKADGLGAYIADVTKTGETPLIIASIAVMSLFVVLMNKFI
                     WRRLYAFAERRFRLD"
     gene            17120..17608
                     /locus_tag="VF453_20855"
     CDS             17120..17608
                     /locus_tag="VF453_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690168.1"
                     /translation="MREPLKWAALSWRLAAWSAGRLASGHNAAIAARQIEPLSLTHML
                     ATLLAEVTSARRAGAYALLWLLALTILNVQTGGAWRSTILFAVPVALVSWKDWRLGFL
                     VAAVAVAAAKYGGAMPEPGSPIPLWLDALVAFAKLSVDALVVNAWGRRQRRRAAPGST
                     RP"
     gene            17605..17937
                     /locus_tag="VF453_20860"
     CDS             17605..17937
                     /locus_tag="VF453_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690169.1"
                     /translation="MKQTIAAIFASPMRANEAVHVLLKAGFAAERRPSIAGDAERTST
                     LRLRDAIGRRLHELLGADATLSPYTQALAAGHFVVKVQATHDAEAAAARRILEIAGDS
                     DVDGRRMA"
     gene            18040..20007
                     /locus_tag="VF453_20865"
     CDS             18040..20007
                     /locus_tag="VF453_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX7690170.1"
                     /translation="MTRPEVSDGADAAATSQETGASRAGAPGANPRRAVVALAALGIV
                     FGDLGTSPLYALQEAFHPGRGVVATPDNIVGALSLFLWSLLAMVSFKYVFLLMRADND
                     GEGGILALLALLTGGASPRGAAGRRGLWLALGLVGTAMLYGDGAITPAISVLSAVEGL
                     SVATPAFAPYVVPLTVVILATLFAFQSRGSGRMGVAFGPILALWFLVIALLGVRACWE
                     APEVWCALDPREAASFFRHNGFRGFEALGAVVLCLTGGEALYADMGHFGRGPIRLAWY
                     GLALPALVLNYAGQAALLLRHPGLADHPFYAMVPAWALYPMVAVATLATIVASQALIT
                     AVFSLTQQAMLLGLWPRMTVVHTSSSTRGQIYMPALNALLAALTIAISIGFGSSDRLA
                     AAFGLSVSATMAITTILFAALASQRWHWPFWRIALVAAPLLVADTAFTAANVLKFSQG
                     GWLSFSLALITGSVMQVWRSGSAEVAASRRGSGFSIDDLVESLRAHPPLRVPGTAVFL
                     CENSREVPGVLLHHLKHNQVLHEEVLLLTLAAADVPYVPAAQRFSARSLDLGVSCCTA
                     RFGFMESVDVPSLLRQGRGLPGFPSVDAEMSTSYYLGRDSLRIAPHWNPLRNWALRAY
                     AVMRRNQLEATAHFGLPANRVVELGARVEVS"
BASE COUNT         3186 a         7057 c         6931 g         3152 t
ORIGIN      
        1 gcgactcgat cgcgcgcgcg agcgccgcgc tcgcctctgc ggcgatgtcg acgaacgcca
       61 cgtgcgcgcc ctgcgcggcg aaggcctcga ccagcgccgc gccgatcccg gagccgccgc
      121 cggtgacgaa cacgcgccgg ccgcgcaggc tgtggaaggt ggcgacctgc atcggcgggc
      181 gggcgtcgga cgcgttcgga agggacgggg aagcggtcat ggatcgatcg attggtgttt
      241 cggtgatggg cggcgacgcc gctcgcggcg cgccggaatt caattccctg agcaatacgt
      301 gttttgcaat gtcagcggcg tttatcttta tttttcgatt atcagtgtgc ggcgatacga
      361 tccgccccgt caagtcgaga agagaagccc catggagacc aagaagcccg tccgccgcag
      421 ccaggcgtgg ttcggcaaga acgatcgcga cggcttcgtc catcgcagct ggatcaagaa
      481 ccagggctac ccgcacgatg aactcgatgg ccgtccggtc atcggcatct gcaatacctg
      541 gagcgagctc acgccgtgca acggccactt ccgcgagctc gccgaattcg tcaagcgcgg
      601 cgtctacgag gccggcggct tcccgctcga gttcccggtc atgtcgctcg gcgagacgca
      661 gctgcgtccc accgcgatgc tgttccgcaa cctcgccagc atggacgtcg aggagtcgat
      721 ccgcggcaac ccgatcgacg gcgtcgtgct gctcatgggc tgcgacaaga cgacgccggc
      781 gctgatgatg ggcgccgcga gctgcaacct gccgacgatc ggcctgtccg gcggcccgat
      841 gctcaacggc aagttccgcg gccaggacat cggctcgggc accggcgtct ggaagatgag
      901 cgagatggtg cgcgccggcg agatgacgca ggccgacttc accgccgccg agagctgcat
      961 gcaccgctcc aagggcagct gcatgacgat gggcacggcc tcgacgatgg cctcgatggt
     1021 ggagtcgctg ggcatgtcgc tgcccgagaa cgccgcgatc cccgccgccg acacgcgccg
     1081 caaccgcctc gcgcagctca cgggccgccg catcgtggag atggtcaaag aagacctcac
     1141 gatgtccaag atcctcacgc gcaccgcgtt cgagaacgcg atccgcacca acgccgcgat
     1201 cggcggctcg acgaacgcgg tgatccacct gctcgcgatc gccggccgca tgggcgtcga
     1261 cctgaagctc gccgactggg acaagctcgg cgccgaggtg ccctgcctcg tcgacctgca
     1321 gccttccggc cgcttcctga tggaggactt cttctacgcc ggcggcctgc ccgcggtgct
     1381 gcgcgagatc tcgcacctgc tcaagctgga tgcgccgacc gtcaacggca agacgctcgg
     1441 cgagaacatc gccgacgcgc cgtgctggaa ccgcgaggtg atccggccgc tggacaagcc
     1501 gctcgtggcc aacgccggca tcgcggtgct gcgcggcaac ctcgcgcccg acggcgcggt
     1561 catcaagccg tcggccgcgt cgccgcacct gctcaagcat cgcggccgcg cggtggtctt
     1621 cgagaccatc gaggaattcc acgcccgcat cgacgacgag gacctggaca tcgacgagca
     1681 ctgcgtcatg gtgctgaaga actgcggccc caagggctac cccggcatgg ccgaggtggg
     1741 caacatgccg ctgccgccca aggtgctgcg caagggcatc acggacatgg tgcgcatctc
     1801 cgacgcgcgc atgagcggca ccgcctacgg caccgtcgtc ctgcacacct cgcccgaggc
     1861 ggcggccggc ggcccgctcg cgctggtgga gaatggcgac atgatcgagc tcgacgtccc
     1921 cgcgcgcagc ctgaagctgg aggtctccga cgaggagctc gccgcccgcc gcgcccgctg
     1981 gcagccgctg ccgcggccgg agcgcggctg gcagaagctg tacgtcgacc acgtgcagca
     2041 ggcgcacctg ggggccgacc tcgacttcct cgtcggctcg agcggagcgg gcatcccgcg
     2101 cgactcgcac tgacggggct cgatactcca cccccgtacc cgcatcgatg gccggacgtc
     2161 cgcagcagct ctcccaaccc cgcgtcctga gcgacgtccg cgccaccctc ggcgaaggcc
     2221 tgtgctggtc gccgcgcgcg caggcgatct ggtcggtcga catcctgcgc caccggctct
     2281 accgcgacac gctcgccggc gagcacctcg agtggagctt cgacgagacg gtctcggccg
     2341 tcgccgagcg tgcgcagcac cccggcctcg cgatcgcgct gcgccgcggg ctcgcgctgt
     2401 tcgaccccgc caccggccag acgacgcgcc tgcacgagcc cgaaccggag cgcaccggca
     2461 accgcttcaa cgacggcaag tgcgacgcgc gcggccgctt ctggggcacg acgatggact
     2521 atgcggtcca ggccgccgcc ggcgcgtact accgctacga cgccgccggc cgcgccgagc
     2581 gcgtgctgga ctgcggctgg gtcgtcacca acggcccgac ctggtcgcgc gacggccgca
     2641 ccatcttcct caacgagacc gcgcagcgcc gcgtccacgc cgccgccttc gacatggaga
     2701 ccggcgccat cggcccgctg cgcgagttcc tgcgcctgtc cgagcccgac ggccaccccg
     2761 acggcatgac caccgacgcc gaaggccgcc tgtggatcgc ccactggggc ggcgcctgcg
     2821 tcacctgcca cgacccggac accgccgagg agctcgcccg catcgcgctg cccaccgacc
     2881 acgtgagcaa cgtctgcttc ggcggccccg acctgcgcac gctgttcgtg acgacggcgc
     2941 gcttcgagct ctccgaggcg cagctcgccg cccagccgct cgcgggcgcg ctgttcgcgg
     3001 tggaggtcga cgcgcagggc gtggcgccga acctgtacgc cggctgaggc gcgccggccc
     3061 cccgaacggg tgtggccccg atgctgcccg caggccagac tgcgggcatg aatcccatca
     3121 acctcccgga gatcgcgcgc acggcgggcg actggttcgg cgtccagggt tccgtcctct
     3181 acgccgtcgc cctggccatc gcgctcggat ggttctgctg gcgcacgcgc tccatccacc
     3241 cggtcaccgg atggatgtgg cgcgtgttcg tcggcaagga cgacttccag gatcccgacc
     3301 tgaagcgctt cctcgacacg cggcagaagc tgctgaaatt ccgcttcgtc accggcatcc
     3361 ccgcccggac ctgggacaag gccaaggaca tcatcgcctg gggcgaggac aacggcgagg
     3421 aactcggcga catcgccgcc tgcggcgccc acttcgacaa ggagcgctgc gagctgaagg
     3481 gcaagcggcg cggcctgctg tggcagatgg ccggctggct gctcctgcag gtgcttttcg
     3541 tcgtgctgct gaccgcgctc ttcgcgctcc accgcatcga ccgcgcggtg ctgtcgatga
     3601 agaccagcgg cacctggatc gcggcgtcgg ccgaccaggt caaacggatc tccggcaacg
     3661 aggcggtcac cgcgcaggac tgcaagcccg acgccggccg ggtcgcggcg cgcttgtccg
     3721 tcacgcccga cgaggccaag gcgatctgca acgccttctc ggacaggaag tttccgaagt
     3781 tcatcgagga caacgtcgcc gagcagcgcg gcctcgcggc ccgcggcgcg tcattcttcc
     3841 tgttcctcgc cgcgctcggg ctgctcggga tccggcacgc cggcgcggcg atcgacatgc
     3901 ggcagcgcct tgaccgaaag aacggcgcgg cccgggcggg tggcgccgaa gccgacgtgc
     3961 gggtcgagct gccggcgctg cagatcaccg tgcaggccga gctgaagtca gctgcgccga
     4021 cgccccggcg gcgatctcgt ccaaaggccc ccgggacgct ggcctgacgc ggcggcctgc
     4081 aggctgcgcg gcgtcgtcgc caaggtcgat accggtgaac gacaactgca cacgattgcg
     4141 gatcacattc gaacattcag cgactgttca gccatatcga cttcgtgtcg caagggtcgc
     4201 gtgacacaat gtttggacac aagggataga tgtccagacc gcacatggtc cgcagcgcgg
     4261 agatgcgccc ggaaatcaac tggcatccaa tgcattccgc attcaagcgg agaaccgccg
     4321 ggcactgcgc gacgcgcttg actcgggata acgacaagtt cgagggacga cagcagcgac
     4381 gcgagtcgcc ggaggccccc tacgctcgcc acgagttggc cacgccactg ctgccgcagg
     4441 aggaaagccg tgttgattgc gagtgtgctg ctgctggtcg tcaccgcgcc ggtcgccttg
     4501 gcttcagggg tatttgccct gcagatcctt ctcggatcgt cgaagcgcag tgggaagatc
     4561 gacgccatcc cggtacgacc aaccggagcg atcgcggtcg tggtgcccgc ccacgacgaa
     4621 gaacgaggca tcgcgcgtac cctgctgtcc atcagggcgc aattgcgatc cggtgaccgg
     4681 ctggtcgtcg tcgcggacaa ctgtgcggat gccaccgcca gcgtcgctcg cgatcacggc
     4741 gcaacggtca tcgagcgaac gcacgcgacc ctgcgcggca agggattcgc cctcgccgcc
     4801 ggcatcgatg cacttcgtgg cgatccaccg gccatcgtgg ttctcgtcga cgccgactgc
     4861 gagctcgcgc ccggtgcggt cgatgcgctg gcaagggcaa cgacgcatgc cgggcgaccg
     4921 tcgcaagcgc gctatctcat gcttcacccc gaaggcgcca gccttccgaa gcgcctcgcc
     4981 cagttcgcct ggcgcgtgcg caattgggca aggccggccg ggtggcatcg catcggcgct
     5041 ccgtgccagc tcatgggaag cggcatgtgc ttccgctggg agactttgcg ggatgcgccg
     5101 atcgccaatg actcgatcgt cgaggacatg cgactcggca tcgacctggc gctcatgggc
     5161 cagccgccgg tgttctgtcc ggaggccctg gtcacgagca tgtttcccga ctccgccgcc
     5221 gcgtcgaaga ctcagcgcat gcgctgggag catgggcatc tcgaaatgat cgcgcgttcc
     5281 gcccttccgc tgctgtggca ggggctgcgt cggcgcgaca agcatctcgt gctgatggcc
     5341 ctcgacctcc tcgtgccgcc gctcgccctg ctgggtgcgc tcctgacatc gctgtttctg
     5401 gcatccctcg gccttgcctg gatcacccac gcgaccgaag cggcgtggat gaacggcgca
     5461 ctggtcgcac tctttctggc cgcggcgctg caatcgaggc gcgcatacgg acgagacttg
     5521 gtcgacttgg tcgaactcgc cgcgattccc gcctatatcg cccgcaagtt gccgatgtat
     5581 gggcgattcg tgaccaagcg acagcgaggc tgggttcgga cggatcgcga cgtccagtga
     5641 acgatgcctt ccacgcccat gcgcgcacgt tgacaacgag tcgttgcggc tcgcgtgcaa
     5701 gggcgctcgg caagcgacgg gagagccgga ttcgcgccgg cctttggagt tatcgggccc
     5761 ggtaagcgcc cgcagtcagc gggacgcgcg ttcgccctga gcaacttctg tccgttgccg
     5821 gggtattgtc ccgcggcgga acgtcatcgc ggcgtcgccg tcagcctccc gatagaggat
     5881 ctcgtgggca tcgacgcact ctcgtgcggc tgccgcagcg agatcgctgc acatgctcat
     5941 attgcggcgc aagacctgac cccgaagccc gcggccccaa ggcccgcagc gccacaggca
     6001 gaccttcctc gcgtccgcgt ttcgacttct gcttccgacc gaggctgtgt gaaaaccccg
     6061 gtccgcaaat gaggccagcc gcgtagagtt tggaagcata tcgagaagga gtcgatatgc
     6121 ccaggttcat cgaaggccag gatcggcacc aggtcacgct gctgcccgag tcgttggacg
     6181 acttcatcgc cgaggacaac gccgtgcgcg tcgtcgatgc gttcgtcgaa gagctggatc
     6241 tgcagtcgat gggcttcgag ggcacggcgc cggccgcgac tggccgccct tcgtacgacc
     6301 cggcggtgct gctcaagatc tacatctacg gctacctcaa ccgcgtgcag tcgagccgcc
     6361 gactggaacg agagtgccag cgcaacctcg aggtgatgtg gttgacggga cgcctcgcgc
     6421 cggacttcaa gaccatcgcc gacttccggc gcgacaacgg caagggaatc cgcgccgtgt
     6481 gccgccgctt cgtcgagctg tgccgcgacc tgaagctttt ctcgcaggcc gccgtggcga
     6541 tcgatggcag caagttcaag gcggtcaaca gtcgcgaccg caacttcacg ccggccaagg
     6601 tcgaggcgcg caagaagcag atcgaggaga gcatccagcg ctacctcgat gccctggacg
     6661 ccgccgaccg gacgcagccc accgaccagg caatagatcg ccaatagccg tcgatgaacg
     6721 tgctcgtccc gggcaagcat aaggcgggta agtccgagcc gcctggagca gcgcgcgccg
     6781 acgacgatgc tgtcgtgcca gcgccgcgac ttcgacgtca ggctggccag ctccgcgagc
     6841 acttgtccct ggaacgccaa cagcgccatg gtgccgagtg actggatgct gagacacgcg
     6901 accagtccgc ccaacacgga cattccatcg agacccagca acgggccgag gaccacggcg
     6961 acagcggcgt gatcaacaac ccactgcgcc gtcgtcgaca tctcctgctc gcctcggtcg
     7021 aacgcgacgt cggcgcagag gcgcggacgc ccgcccggac tggtcagcga tggtcatgga
     7081 cggacggcgc cttctcgcag ccgtcgtcat gcgcgtgatc gccatccgca tgtggcgtga
     7141 acgccgccgt gacctcgatc tcgaccttgt agtcggagtt gatcagcttg gcgccgatgg
     7201 tggcgcgcgt cggcgtgtgg ccggggacga cccagtcgtc ccacaccgcg ttcatctgcg
     7261 cgtagtcgcc gaccgaggcg agatagacgc gcgcggagac gatgtgctcg cgccgcgccc
     7321 cgacgtgcgt caggaggcgg tcgatgaagc cgagcacctc ccgcgtctgg ccgccgatat
     7381 ccaacgcggt gttctccgcg acttggccgg cgatgaatac gagcttgccg gtgccgacgt
     7441 cgacgacggt catctcgctg aaacgggggc cgatctcgtg acgttcgatg tgcatggctt
     7501 gtctccggat ctggatgggg tgcgtcgagg cgaactcagt cgcgcgcggc ttgggcatgt
     7561 tcgcggttcc accagccgcc gcccagcgac tgcagcagcg cggcggcgtc ctgcaggcgc
     7621 tgcgcacgcg cctggacgta cccgacgagc gcctgctggc gtgcctgctc cgattggctc
     7681 accgccagcg cgctgatgtc gccgagctcg agctggcgcc gcgcgatcgc cagcgtgcga
     7741 tccgcgagat cgagcgcatg ccaagtcgcg cgctcggcct cggcgtcggc ctgcaccgcc
     7801 tgcagcgcgt cggcgacgtt ttgaaaggcg ttgatgaccg tggcgcgata ggtcgcctcg
     7861 gcctggtcgt aggccgcccg cgcggcgtcc tccttgtgct tgagcgcacc gccgtcgaac
     7921 agaggctgcg tcacgccgcc ggccagcgtc cagaacgacg tgcccggcgc gaacaggccc
     7981 gacagcgacg ccgccgacga gccccaggtg ttcacgccga gcgtgacctg cggcaagcga
     8041 ttggcgacgg ccacgccgat gtcggccgag gcgctttgca actgcgcttc ggcggccagc
     8101 acgtcgggcc gatgctcgac cagcgtcgac ggcagcgtca acggcagcgg gtcgggcagc
     8161 gtcaggtcgt ccagcgtcgg ttcgggtacg tcggcggtcg gcggcaaccc cgccagcgcc
     8221 ttcacgagat cgcgctgctg caccatctgc ttgtagacgc ccggcagcgt ggcctgcagc
     8281 gcggcgagct gggactcctg ctgcgccacg tcgagctcgg agttctcgcc caggcgctcg
     8341 agctcgcgaa aacgcgccag cacctccgac tgcagcgcga tctgccgcgt cgtggcctcg
     8401 aactgcgcgt gcaggctggc ctcggtgatc accgcgacga cgaggttgga gctcagcgtc
     8461 aggtaggtcg cctcgacgtt ccaacgctgc gcctgttcct gcgcctgcag cgattcgacc
     8521 tggcgccggt tcatgccgaa caggtcgggc gtgtacgaga cgctcagctg cgcggtgctg
     8581 aggttgaaca ggtcggcatt ggacgcgagc gggctggcga gcggacgggc gaccttctgc
     8641 cgcgaggcgc tataggacgc gtcgacggtc gggaagaagc tcgcctgctg cgcccgccgc
     8701 agctcggtgg cctggcgcag cgcggcctgc gccgcggcga ccgacgggct gcgcgccagc
     8761 gcgccgcgca ccagttcgtc cagcgcgggc gaatggaaca gcgtccacca ttgcgcgggg
     8821 aggtcggcat ccacgacctg ccgctcgtcg cgcatcgcgg cctcgaccgg cgcagggagg
     8881 taggccgccg cgggcgattc gggtcggtgg tagtcgggcc cgaccgcgca gcccgcgagc
     8941 cacgcgacgc tgccgatggt cagaccgatg cgatggcaaa aatggagttt ggacatcaac
     9001 gcgtcttccg catcttttga tcgcgctcgc ggctctcgaa gtgacgttcg attgcgaagt
     9061 agaggacggg cagcacgagc agcgtcagca gcgtcgacac gaccagcccg ctgacgacga
     9121 ccgtggcgac gccacgctgc acgtccgtgc ccacgcccgt ggccagcgcc gcgggcagca
     9181 tgccgatcga ggcgacggtg gccgtcatca gcaccggccg caggcgctcg gtcgcagctc
     9241 gcacgacgga ctcgagcagc gcatagcctt cgccgcgcac gcggcggaag ttcgcgacca
     9301 tgatgatgcc gttctgcacc gacacgccga acagcgcgat gaagccgacc gccgtggcca
     9361 cgttcagcgt ctcgccggtg atccgcagcg cgatcaggcc gcccagcgtg gccatcggca
     9421 ccacgcccag caccagcagc gtctgccgaa ccttgccgaa ctggaagaac agcagcacgg
     9481 ccatcatcgc gaacaccagg ccgaggatga acacgagccg ggcctgcgcg cgccgctcgt
     9541 tctcgaactg gccggcccac tcgaaatggt gcttggtcgg atcgaacttc acctcggcgt
     9601 cgatgcggcg ctgcgcctcg gccaggtagg acgtgaggtc gcggtcgcgg ttgtccacgc
     9661 gcacggtgat ctggcgctcg ttcatctcgt gcgagatcgt gctctcgccg gtcttgtact
     9721 ggatcgacgc cagctgcgac aacgggatgc gcgcgccgcc cggcgcggtc agcgtgagcg
     9781 cgcccagatc ctgcagcgag cccttggccg agcgcgggaa cttcaccgtg acgttgtatt
     9841 gccggtcttc ctggtacagc gtcgtgacgg ccgcgccgcc gatgccggtc tggatcagcg
     9901 ccgagatgtc cgacacggga atgcccacgc gtccggccgc ctcgcggtcg accttgacga
     9961 ccagctgcgg gatctgcggc tcctggaata tcgaggccga ggccgtaccc ctgaccgtat
    10021 gcaggatgtc cacgacctgg ctcgcgatgc ggcggtcgtc ggcgatgtcc tggccgtaca
    10081 cgcgcattac cagcgggctg tgcgcgccgc cgatggcgtc gttgacgccg tcgatgatgg
    10141 gctggctgat gccgacgtcg aagcccggga tgcgctgcag cttgtcgttg agccggcgca
    10201 cgaactcggc cttgctctcg tggtcgggcc actccgcgta aggcttgagg ccgacgggca
    10261 cctccatgtg tgacggcgtc cacgggtcgg tgccgtcgtc gttgcggccc agctgcgtga
    10321 cgacgtacga gatctcgggg aactcgcgca gcgtgcggcg cagctcgctg gccatctcgc
    10381 tggccttgtc cagcgacagg cccgtgggca tctgcacctg cagccacagc gcgccttcgt
    10441 cgagctcggg caggaactcg cgtccggccg tcgcgccgag cacgccgacc gcggccagca
    10501 ccagcaacga cacgagatag atcaccggcg gccggcccag cagccagccg agcgcgttgc
    10561 ggtagcggtc ggtcaaccag atcagcggcc ggttgtggaa cacgcggcgc ggcttgcgca
    10621 gcgcgacgaa ggccaggccg gggatcagcg ccatgcagcc cagcagcgcg ccgaacagcg
    10681 cgaagccgac cgtgaaggcc atcggcgaga acagcttgcc ctcggcgcgc tcgaacgcga
    10741 acagcgggaa gtaggcgcag acgatgatga gcgtggcgaa gaagatgggc cgcgcgatct
    10801 ggccggtggt ctcgagaacg tcgtcctcga ccagcggctg gtcgggctgc tcctcgcgcc
    10861 ggcgcagcat ggcctcggtc accacgatgg cgccgtcgac gatgatgccg aagtcgatcg
    10921 cgccgagcga gaacaggttg gccggcatgt gcagcaggtt catcgcgatg aacaccgtca
    10981 ccagcgcgag cgggatcgcg accgcggcga cgatggcgct gcgcgggctg ccgaggaaca
    11041 ggatcagcac gacgcacacc agccccacgc cttcgagcac ggtgtgcatg accttctcga
    11101 cggtcagctt cacgagctgg tcgcggtcga tgtagggcac gatcttcacg cccagcggcg
    11161 tgagccgctt ccgcagctcg tcgaccttcg cgtgcacgcc ctcgagcacc ttggacggat
    11221 tctggccctt gagcagcagc acgatgccct cgatggtgtc gggatcgctg tccttgccca
    11281 ggattccttc gcgctcctgg tggccgatct ggagcttgcc gagctcgcgc agctgcaccg
    11341 gcacgttgct gctggtggcc accgcgatcg aaccgagatc gtcgaggctg tgcgcctggc
    11401 cgatgccgcg cacgacgtag ctctgctcgc cgcgcgagat gcggccgccg ccggagttct
    11461 ggctgccatt gttgatcgcg gtgacgacgt cgttcagcgc gaggccgtag cgatcgagct
    11521 ggcgcggatc gagctcgatc tcgaactcct tggtgaagcc accgaagttg ttgacgtccg
    11581 cgatgccggg cacctggcgg aacgccggca tcacgaccca gcgctggatc tccgacagct
    11641 gcatcaggtt cttggtgtcc gactccagcg tgtagcgata gatctcgccg accgggctcg
    11701 agatcgggtc gagcccgggc tgcacgcccg cgggcatcgt cacctgcgcg aggcgctcgg
    11761 tgacgcgctg gcgcgcgaag tagtcgtcgg tgccgtcctt gaagacaagc gtgatcagcg
    11821 acagcgcgaa ggtgctgctc gatcgcatcg tcaccaggcc cggcgtgttg gccagcgcgc
    11881 gctcgagcgg cgtcgtgatc tgctgctcga tctcctcggc ggccaggccc ggcacctggg
    11941 tcgtcacctg cacggtgacg tcgctgatgt ccggataggc ctccaccgtc atctgcgtcc
    12001 acgagaacca accatagacg aggatcagca gcgtcagcac ccacaccagc aggcgccgcc
    12061 ggaagcacag cgcgatgaac tggttgatca ttgcagcagc actccgcctc gcacgacggc
    12121 ccgctcaccg cgcttcaggc cagccaggat gtgcacggac tgctcgtcct ccgcgccgac
    12181 ctcgaccacg cggcgcacga aggtccacgg cgcgacctcg accagcaccg tcgtgctgtc
    12241 gttgttcatc agcagcgcgg acggcggcac gctgacgccg tcggcctcgg gcagcgcgaa
    12301 ggtggcggtc gcgaaggcgt tcggacgcag gcggccgtcg gcgttgtcga acgggatgcg
    12361 caccttggcg cgccgcgtgt cgggctcgag caccggactc acgcgcgcgg ccttgccgtg
    12421 ccagacctgg ccgacggctc cggccagcgt gacgtccacc ggcgtgcccg gcgcgacggc
    12481 agcggcctgc tgctcgggca actgcgccgt cacgaagacg cggtcgaggc cggacaccgt
    12541 catcagcgac gcggtcgcgt cgttgacgaa cgtgcccgcg ccgacgttga gcgccgtcac
    12601 ggtgcccgcg accggggcga gtaccaccag cgcgtgcgtg cgcgtgtcga cgcgcgtgcc
    12661 gccggccagc gtcgagacgc ggtcgcgggc gcggcgatcc tcagcggcgg cgtcgcgctg
    12721 cgcgagctcg gcggcctcga gatccttgcc cgcgttggcg ccgaccgcct gcaccccctt
    12781 ggcacgctcc agcgcgcggg tcgcaagttc gagcgcatcg gcggccttgg ccgcgtcgtc
    12841 cagcgcctgg ccgaagtcgg gcgacgagat cgtggccagc ggctggccgc ggtgcacgct
    12901 gtcgccaagg cccacgtgca gttcgaccag ccgaccggtc aggggcgcga cgatcgcgac
    12961 cacgcgcgag ggatcggcct cgacgactgc cggcacgctc agccgtcgcg ccccaggccg
    13021 tgtcgtgacc tcggcgacgg ccagtcgctg gcgcagcggc gaatcggcgg gtacaacgat
    13081 cctgccgttc tgctcgacga acatcggccc cggctcagcc atcggctggg cggcaagagc
    13141 ggctgcggcc acggcggcgc cgaaaggagc ggcaagggat cgggcgagga atttcatcat
    13201 gctgtcacgg ctgctagagg gtgcgtcgca ccgcgatgcg tagggccttt cgcgagcgag
    13261 ggcaaaattg tcgagtctgt agttgctaca gagtcaaccc cgcgccttcc gcagcctgaa
    13321 ggccagatga accagggcct gcaccatgaa gatcggcgac ctcgcgacct ccaccgacac
    13381 gccggtggag accatccgtt attacgagcg cgaaggattg atgagcgcgc cggcgcgcag
    13441 ctcaggcaac ttccgcctct acgacgaagg ccacgtgcga cgcctgggct tcatccgcgc
    13501 ctgccgcgcg ctggacatga acctcggcga gatacgcgtg ctgctgcgct tccgcgacgg
    13561 cggcgagtcc gactgcgggg aggtcaacgc gttgctggac gagcacctcg agcacgtcga
    13621 ggagcgcatg cgcgagctgc ggcgcctggc caaggacctg cgcgcgctgc gcgacgagtg
    13681 ccgcaaggcc agtccgacgg atcagtgcgg catcctgcgc ggcctggaca ggggcgcgtt
    13741 cgaggcgccg gccaagacga agtcgcacgt cgggcacgtg catcgcaaag gcggcaagag
    13801 ctgacgccgc ctcatcagcc tggcgcgtcc tcgtccttgg gcacgaagac cttgttcgtc
    13861 ttcgggttca ggtccatcag gccggcatag cggccccagg cgatcatggt ctcgaaggtc
    13921 ttctcgggat tgtcgtaggg cagcgcgctc gccacgtcct tgatgacgcg ctcggcctcg
    13981 atctcctcat actcgcccag cagcgcgatg atgatgtgga aaatgcgcag cttgtagacc
    14041 tgctccgcga agatcttgcg gcggccttcg cggtcggagg cgatgaagcg cttacccaac
    14101 tcggtcagtt cgacgtcgtg cttgggagtg tcgacgaagt cgaggatctc cgcggccttg
    14161 acgatggaga tcgtctcgcc gaactccttg ccgatgtcgt cggagatctc gtagatgttc
    14221 ttcagctcgg gcgcgccgtc gaggatctcc agcagcccca ggatctgggc gaccggcacc
    14281 tgcgggatcg actccatgcg cgagcgtgcg cgcgagacgg cctgcccggg cgccggcggc
    14341 gcctcgggcg gaaggtcggg cagcgtcagc gtggtgatgg tctcgtggat gatgcccacc
    14401 agccgctgga acccggcgct cttcgggtcg cgcggatacg gcagggggtt gtccagcacc
    14461 aggcccagcc ggcccgggcg cgggaagacg accacgatgc gcgtggccat ctcgaccgct
    14521 tcctcgatgt tgtgcgtcac catgaagatc gagctgatgc cgttctcctt ggaccgccac
    14581 aacgcgatga gctcctggcg caggttctcg gcggtgagca cgtccagcgc gctgaaggcc
    14641 tcgtccaggc acagcatctt gggctgcgag accagcgcgc gcgcgatgcc cacgcgctgc
    14701 cgcatgccgc ccgagagctc gcgcggatag gccgcgtggt aattacccag gccgatcagg
    14761 tcgatggcgt ggtcgaccgc ggcctcgcgc tcggcgcgat ccatcttgcg cgacatcaga
    14821 cccaccgcga cgttctgctc cacggtcagc cacgggtaga gcgcgaaggt ctggaagacg
    14881 atggacacgt ccgggttgat gccctgcagc agcttgccgt tgcgcagcgc gcggcccgag
    14941 gtcggctcga ccaggccggt caggcagcgc aggatcgtcg acttgcccga acccgactgg
    15001 cccagcagcg cgagcagctc gcccggccgc acggcgagat cgatgttctc cagcaccttg
    15061 aggtcgtgcc cgccggcggt cttgtacgac ttcgagactg cgcacagctc ggcgacggga
    15121 acggcgggcg cggcggtggc ggtgggcatg tcggtcatgg tcaatccagt cggaagcggc
    15181 gctcggcgaa cgcgtagagg cggcgccaga tgaacttgtt catcagcacg acgaacagcg
    15241 acatcaccgc gatgctcgcg atgatcagag gcgtctcgcc cgtcttggtg acatcggcga
    15301 tgtaggcgcc gaggccatcg gccttcagcg tcgtgtcgcc gaaggtggcg agctcggcga
    15361 cgatgctcgc gttccaggcg ccgccggccg cggtgcaggc gcccgtgacc cagaacggga
    15421 agatggccgg caggatcagc gtgcgccaga gatcccagcg acgcaggccg tagacgcgcg
    15481 cggcttcctt caggtcgttg gggatggcca tcgcgccggc cacgacgttg aacaggatgt
    15541 accactgcgt gcccatcgcg atcagcagga tgctgcccca gttgatcgag atgtgcgtcg
    15601 cgacgaacca gccgacgacc accgggaagg tcatgttgac cgggaacgac gcgccgatct
    15661 gcgccagcgg ctgcgcgaag cgcgcaacct tgggctggaa gccgatccac acgcccacgg
    15721 gtgtccacac gagcgtcgcg agcacggtca tcgcgcccac gcgcagcatc gtgagcaccc
    15781 ccagccaggc gatgtgcgcg accagcttcc acgacagcgc gccgcgcagt tcctcggctg
    15841 ccgcgacggc gccttcgatc atctcgtagg ccagccacgc gaccgccacg accacgacga
    15901 cacgccagac atggtcggcg cgcacgaccc gcaccgaagc cagccgccgc gcctggcggc
    15961 gcccgatcga cgcgccgtgc tcggccaacg gacgcacgat gtgctcgccg agccacgtga
    16021 agacgtaggc accgcgcagc aggtcgtaga cccaggacgt cgccggcatc tgcgatacgg
    16081 tcagctcgat cttgaacttg tccgcccagg cgagcagcgg ccgccacacg agttggtcgc
    16141 tcacgaggat cagcacgatc atcgcaacga ccgcccacag cgccgcgttg ttgctgccgg
    16201 cggcgatggc ctgcgccatg taggagccca ggcccggcag cttgatgtcc ttgttcatca
    16261 cggtgatggc ctcgctctgc gcgacgaaga accagccgcc gccgaacgac atcatcgagt
    16321 tccagatcag gctgtgcgcc gaggcgggca gttccaccgt cgtgaagcgc tgccagcggc
    16381 tcaggccgta ggttgtcgcg gcctcctgca tgtcctgcgg gatcgtgacc atcgagtggt
    16441 agaagccgaa ggccatgttc cagacctggc cggtgaagat cgcgaagatc gccgcgcact
    16501 ccacgcccat caggctgccc gggaacagcg ccatgaagcc ggtcaccgtc gccgacagga
    16561 agcccagcac cggcaccgac tgcagcacgt ccagcgccgg caggatgaac atccgcgccc
    16621 gcgcgctctt ggccgccagg tagcccgtgc ccacggcgaa cagcagcgag aacgcgaacg
    16681 cgatccacat gcgcagcaac gtgcgccccg cgtagtacgg gatcatcgag acggacaaat
    16741 cgatggcagg ctgcgacgcg gcgtcgaagt gcaccagcat gcccttgccg aagtgcagcg
    16801 cgctccagaa gaccgccagc agcgccagca tgacgacgcc gtcgacccag gtgaagccgc
    16861 gccgcggctc ggcgacctgg agttggggag cgatgctgcg cttcacgggc ggcacgctaa
    16921 cagatgcgga tgagtggtgc tgtcacgcgc ctgaaagctt gggctgggcc gttccggtac
    16981 gaatcggcgc cgtagctggg gggacgtcct ctcgccccat gcccatgggg cgcagggccg
    17041 atcgtctcgc gcccaatcgc agcctcgcgt gcattgacgc gatcttgttg gccgtggcgc
    17101 gaatctttgc agcttcttta tgcgagagcc gctcaaatgg gcggcgctgt cttggcgcct
    17161 tgccgcgtgg tcggccgggc gccttgccag cggacacaac gccgcgatcg ccgcgcggca
    17221 gatcgaaccc ctgtccctga cccacatgct cgcgacgctt ctcgcagagg tcaccagtgc
    17281 acgccgcgcc ggcgcgtatg cactgctgtg gctgctggca ctgaccatcc tgaacgtgca
    17341 gaccggtggc gcctggcgtt cgacgatcct gttcgcggtg cccgtggcgc tggtttcctg
    17401 gaaggactgg cgcctgggct tcctcgtggc cgccgtcgcg gtcgccgcgg cgaagtacgg
    17461 cggcgcgatg ccggagccgg gctcgccgat cccgctctgg ctggatgccc tcgtggcctt
    17521 cgcgaagctc agcgtcgatg ccctcgtcgt gaatgcatgg gggcggcgac agcgtcgccg
    17581 agccgctccg gggagcacgc gcccgtgaag cagaccatcg ccgccatatt cgcgtcgccc
    17641 atgcgcgcaa acgaggccgt gcacgttctg ctcaaggccg gcttcgcggc cgagcgacgg
    17701 cctagcatcg cgggcgatgc cgagcggacg tcgacgcttc ggctgcggga cgcgatcggg
    17761 cgccgcttac acgaactgct gggcgccgac gccacgctct cgccgtacac tcaggccctg
    17821 gcggccgggc acttcgtcgt caaggttcag gcgacgcatg atgcagaagc cgcagccgcc
    17881 cggcggatcc tcgaaatcgc cggcgacagc gacgtcgacg gacgccggat ggcgtgatgc
    17941 ggagccgatc tacatgcgca cttcccgcac acaccctggc atgagacccg acggatcgac
    18001 gccagtcgcg cgcgcaacgg cacgggagcg accgcccgca tgacgcgacc cgaggtttcc
    18061 gacggcgccg atgctgccgc cacatcgcag gagacgggcg cttcccgggc cggcgcgccg
    18121 ggcgcgaatc cacggcgcgc ggttgtcgcg ctcgccgcgc tcggcatcgt cttcggcgac
    18181 ctcggcacca gcccgctcta cgcgctgcag gaggcgttcc atcccggccg cggggtcgtg
    18241 gcgacgccgg acaacattgt cggcgcgctc tcgctgttcc tgtggtcgct gctcgcgatg
    18301 gtcagcttca agtacgtgtt cctgctcatg cgggcggaca acgacggcga aggcggcatc
    18361 ctcgcgctgc tcgcgctgct aacgggcggc gccagcccgc ggggggcggc tgggcgccgc
    18421 gggctctggc ttgcactcgg gctcgtcggc acggcgatgc tgtatggcga cggcgcaatc
    18481 acgccggcga tctcggtgct cagcgccgtc gagggcctgt cggtcgccac gccggccttc
    18541 gcaccgtacg tcgtgccgct aactgtggtc atcctcgcga cgctgttcgc cttccagtcc
    18601 agaggttcgg gccgcatggg cgttgcgttc ggccccatcc tcgccttgtg gttccttgtg
    18661 atcgccctgc tgggcgtgcg cgcctgctgg gaagcgccgg aggtctggtg tgccctcgac
    18721 ccgcgcgagg cagcttcctt cttccggcac aacggcttcc gcggcttcga agcgctcggg
    18781 gccgtcgtgc tgtgcctgac gggcggagag gcgctgtacg cggacatggg gcacttcggt
    18841 cgcggcccca tccgcctggc atggtacggg ctggcgctgc ccgcgctcgt gctgaactac
    18901 gcgggccagg ccgccttgct gctgcgccat cccgggctcg ccgatcaccc gttctacgcg
    18961 atggtgccgg cctgggcgtt gtatccgatg gtggccgtcg cgaccctggc cacgatcgtg
    19021 gcatcgcagg cgctcatcac ggccgtgttc tcgctgactc agcaggcgat gctgttgggc
    19081 ctgtggccgc ggatgactgt cgtccacact tcctcgtcga cgcgggggca gatctacatg
    19141 cccgcgctca acgcgctgct cgcggcgctg accatcgcga tctcgatcgg ctttggcagc
    19201 agcgatcgcc tggccgccgc gttcggcctg tcggtgtcgg cgacgatggc gatcacgacg
    19261 attctcttcg ccgcgctggc ttcgcagcgc tggcattggc ccttctggcg catcgcgctc
    19321 gtggccgccc cgttgctcgt cgcggacacg gcgttcaccg ccgccaatgt cctgaagttc
    19381 tcgcagggcg gctggctgtc gttctcgctt gcgttgatca ccggcagcgt gatgcaggtc
    19441 tggcgctcgg gcagcgccga ggtcgccgcg tcgcgtcgcg gaagcggctt ctcgatcgac
    19501 gatctggtcg agtcgctgcg ggcccatccg cccctgcggg tacctggcac ggcggtcttc
    19561 ctgtgcgaga actcgcggga ggtgcccggt gtcctgctgc accacctgaa gcacaaccag
    19621 gttctgcacg aagaagtcct tctgctgacg ctcgccgcgg cagacgtgcc ctacgttccg
    19681 gcagcgcagc gcttctcggc ccgttcgctc gatctcggcg tgtcatgctg cacggcacgt
    19741 ttcgggttca tggagagcgt cgacgtgccg agcctcctgc gccaaggtcg gggcctccca
    19801 ggattcccgt cggtcgacgc cgagatgtcg acgtcctact atctgggccg cgacagcctg
    19861 cgcatcgccc cgcactggaa ccctttgcgc aactgggcgc ttcgcgccta cgccgtgatg
    19921 cggcgcaacc agctcgaggc cacagcgcac ttcggcctgc cagcgaacag agtggtcgaa
    19981 cttggcgcgc gagtcgaggt gtcctgattc gctcgggcgg tatcggcctg cccgtccaac
    20041 ggagagggtg tctgcattgg gccggaaggc tgcctggaga gttgcgcgac acgagttcgg
    20101 caatcgctgc ggcactgccg tcgatggttt gccgtcgagt gcgggggcgg acctgcagac
    20161 gagtaccgca tcacgcaatc gcaggctaag gatggtctgc aaaacgtgac acggagcgct
    20221 gcgcagccct gttgatgcca cacgtatcgt caggtcgcat gtctgtcggc ctcgatcagg
    20281 cggcagcgca ggcgcttggc cctgtcgcag ggcgcatttc acgctc
//
LOCUS       DASVLN010000886         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_477387, whole genome shotgun sequence.
ACCESSION   DASVLN010000886 DASVLN010000000
VERSION     DASVLN010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477387"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1138)
                     /locus_tag="VF453_20870"
     CDS             complement(128..1138)
                     /locus_tag="VF453_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX7690171.1"
                     /translation="MHEISIGFGFVAALVALAVLFDFLNGFHDAANSIATVVSTGVLR
                     PQTAVLFAAFFNVVAYAVFHLSVASTVGKGIVDVAFVDQHVVFGALVGAITWNVFTWW
                     VGIPSSSSHALIGGIAGAVIGRGQGAHLMGATIGKTVLFIVLSPLLGFVLGGLTLVAV
                     AWICRRQPPLRVDGWFRRLQLVSAGLYSLGHGGNDAQKTIGIIWMLLIAGHYADARAA
                     EPPAWVPMVCYLSMGLGTMFGGWRIVKTMGQRITKLKPVGGFCAEFGGALTLFFASAL
                     GVPVSTTHTITGAIVGVGSVRGAKAVRWGVAGNIVWAWIFTIPAAGVVAALFAAASGF
                     LF"
     gene            complement(1154..1801)
                     /locus_tag="VF453_20875"
     CDS             complement(1154..1801)
                     /locus_tag="VF453_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX7690172.1"
                     /translation="MWFSKRLPREGNFFELFNQHADRTVEGARAFMAMIRNYDDQALR
                     EKHAAEVDVAERAADKMTAEVHRLLHRTFITPLDGEQIRSLINAMDDVADMLQDSSEL
                     LSLYDVQRISEDVLRLTEISVRCCERVQHVVHLLGRLRDLEVAEAARKTCEEIDHLES
                     DADRVMRAAMSRVFRDETDTRQLIKLKAIYEHLETISDRCQDVANVVEGIILEAS"
     gene            complement(2087..2578)
                     /gene="folK"
                     /locus_tag="VF453_20880"
     CDS             complement(2087..2578)
                     /gene="folK"
                     /locus_tag="VF453_20880"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX7690173.1"
                     /translation="MGANLGDARATLAAALDALRSLAGDPARVAVSSFWRSAPVDAHG
                     PDFVNAVARIETMCSPHALLDALQAIEQRFGRERPYRHAPRTLDLDLLLYGVAGEEGG
                     LVLDDARLVLPHPRAAQRAFVLEPLAELWPAGRIPGLHGGSLAGQLALVRADASQRIE
                     RLD"
BASE COUNT          406 a          944 c          953 g          340 t
ORIGIN      
        1 gcagtcgccg gcgtcgcggg cgaccgcgcg cagcgcgtcg tcggccaggg acgcgaggcc
       61 gctcagctcg tcgatgcgcg cgccggggtc gtcgggatgc ggcgtgtcca tcgcatcccc
      121 gcgcgggtca gaacaggaat ccgctcgcgg ccgcgaacag cgccgcgacc acgcccgcgg
      181 ccgggatcgt gaagatccac gcccacacga tgttgcccgc cacgccccag cgcacggcct
      241 tggcgccccg caccgagccg acgccgacga tggcgcccgt gatcgtgtgc gtcgtcgaca
      301 ccggcacgcc cagggccgac gcgaagaaca gcgtcagcgc accgccgaac tcggcgcaga
      361 agccgccgac cggcttcagc ttggtgatgc gctggcccat ggtcttcacg atgcgccatc
      421 cgccgaacat cgtgcccagg cccatcgaga ggtagcagac catcggcacc cacgccggcg
      481 gctcggcggc gcgggcgtcg gcgtagtggc cggcgatcag cagcatccag atgatgccga
      541 tggtcttctg tgcgtcgttc ccgccgtggc cgagcgagta cagccccgcc gacaccagct
      601 gcagccggcg gaaccagccg tccacgcgca gcggcggctg gcggcggcag atccaggcga
      661 ccgcgaccag cgtcagcccg ccgaggacga agcccagcag cggcgacagc acgatgaaca
      721 gcaccgtctt gccgatggtc gcgcccatca ggtgcgcgcc ctgcccgcgg ccgatcaccg
      781 cgcccgcgat gccgccgatc agcgcgtgcg agctgctcga cgggatgccc acccaccatg
      841 tgaacacgtt ccaggtgatc gcgcccacca gcgcgccgaa gacgacgtgc tggtcgacga
      901 acgccacgtc gacgatgccc ttgccgaccg tcgacgcgac cgagagatgg aagacggcat
      961 aggcgacgac gttgaagaac gcggcgaaca gcaccgcggt ctgcggccgc agcacgccgg
     1021 tggagacgac cgtggcgatg gagttggccg cgtcgtggaa gccgttgagg aagtcgaaca
     1081 gcaccgcgag cgccaccagc gccgcgacga agccgaagcc gatcgagatc tcgtgcatgg
     1141 ggatccgtcg cgctcaggag gcttcgagga tgatgccctc gacgacgttg gcgacgtcct
     1201 ggcagcggtc ggagatggtc tccaggtgct cgtagatcgc cttgagcttg atcagctggc
     1261 gcgtgtcggt ctcgtcgcgg aacacgcgcg acatcgccgc acgcatcacg cggtcggcgt
     1321 ccgactcgag gtggtcgatc tcctcgcagg tcttgcgcgc ggcctcggcg acctcgaggt
     1381 cgcgcaggcg gcccagcagg tgcacgacgt gctggacgcg ctcgcagcag cgcaccgaga
     1441 tctcggtcag gcgcagcacg tcctcgctga tgcgctgcac gtcgtacagc gacagcagct
     1501 cgctcgagtc ctgcagcatg tcggcgacgt cgtccatcgc gttgatgagg ctgcggatct
     1561 gctcgccgtc cagcggcgtg atgaaggtgc ggtgcagcag gcggtgcacc tccgccgtca
     1621 tcttgtcggc cgcgcgctcg gcgacgtcga cctcggccgc gtgcttctcg cgcagcgcct
     1681 ggtcgtcgta gttgcggatc atggccatga aggcccgcgc accttccacc gtgcggtcgg
     1741 cgtgctggtt gaacagctcg aagaaattgc cctcgcgcgg cagccgcttg ctgaaccaca
     1801 tggtgtcgtc ctcgtgaact ctcggaatgg ctcggccgcg cgctgggacg cgaggccggc
     1861 ggtgggtgtc gggatgggaa ctcgggcgga cgccgggtcg ggacggaggt cccgcggtcg
     1921 gcccgcggcc gcgcggcgag gcggcggcgg gcggtattgt acgggcggcc gtcacgaaat
     1981 cgtcacgaga cgccgccgca cggcgtgcgc aagtgcgcgg cggccgcggc gcgcatcgcc
     2041 ggcggccggg tcagaacctg tgaatgaatc gagcgcgtcc gagttctcag tccagccgct
     2101 cgatccgctg cgaggcgtcc gcccgcacca gcgcgagctg cccggccagc gagcccccgt
     2161 gcagcccagg gatccgcccc gcgggccaca gctcggccag gggctccagc acgaacgcgc
     2221 gctgcgcggc gcgaggatgc ggcagcacga gtcgcgcgtc gtcgagcacc aggccgccct
     2281 cctcgcccgc gacgccgtac agcagcaggt cgagatccag cgtgcgcggc gcatggcgat
     2341 acggacgctc gcggccgaag cgctgctcga tggcctgcag cgcgtccagc agcgcgtgcg
     2401 gcgagcacat cgtctcgatg cgcgcgaccg cgttgacgaa gtcggggccg tgggcgtcca
     2461 ccggcgcaga acgccagaac gacgacaccg cgaccctggc cggatcgccg gccagcgaac
     2521 gcagcgcgtc cagcgcggcg gcgagcgtcg cgcgcgcgtc gccgaggttg gcgcccagcc
     2581 cgacgaacgc ggtcgcgggc gcgccggatt cagtcgccgg agccgtcgtc ggcgtcgccg
     2641 tcg
//
LOCUS       DASVLN010000887         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_477422, whole genome shotgun sequence.
ACCESSION   DASVLN010000887 DASVLN010000000
VERSION     DASVLN010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477422"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VF453_20885"
     CDS             complement(<1..483)
                     /locus_tag="VF453_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7690174.1"
                     /translation="MQTIEVGGRLLLALAHHGQPLALKDLAQAAAMSPGKAHPYLVSF
                     IKLGLVERETEGGRYGLGPLALQMGLIGLQQYDPVRLATVRLDELARATGHTVAIAVW
                     GHRGPTLVRVAEAPSPVHVTMRHGTVMSIEGTASGRLFAAWGPDEAVREALAAEAALA
                     P"
     gene            658..1989
                     /gene="hmgA"
                     /locus_tag="VF453_20890"
     CDS             658..1989
                     /gene="hmgA"
                     /locus_tag="VF453_20890"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX7690175.1"
                     /translation="METSSSTAAGNDALHAGFGNHFSSEAVPGALPQGRNSPQRAPMG
                     LYAELLSGAAFTAPRHANVRTWLYRRQPSVRCGPYQALEHPLLRTGARDGVDVAPDPL
                     RWHPQPVAAGAGRDFVDGLRTMVVNGDPDMQVGVAVHLYGFDRPMGERAFACADGELL
                     LVPQDGALRVTTELGVLHAGPGEIVLLPRGLVFKVDPAVDGAACNGYACENYGAAFRL
                     PELGPIGSNGLANTRDFIAPRAAFDDGPARPWTLVRKFGGRLWQAPAQASPFNVVAWH
                     GNLAPYVYDTRRFNTIGSVSFDHPDPSIFTVLTSPSDTPGWANADFVIFPPRWMVAED
                     TFRPPWYHRNVMSECMGLVYGQYDAKEEGFRPGGLSLHNSMVPHGPDADAFDKATSAA
                     LAPRKLDNTLAFMVESRWRLRPTAFALQGGALDASYADCWAPLRDHFTPGN"
     gene            1991..3310
                     /gene="fahA"
                     /locus_tag="VF453_20895"
     CDS             1991..3310
                     /gene="fahA"
                     /locus_tag="VF453_20895"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HEX7690176.1"
                     /translation="MLDFTHDPQARSWVDSAEAPDADFPLQNLPFGRFRRAATKEAAR
                     VGVAIGDRILDLAALGELDAQVAPLLGPLLHGDLNAFMAQGRAARIALRHALFAGLSA
                     EPSGTASLWQARRDTLLVPQDEAEMALPCRIGDYTDFYTGIHHATTVGRLFRPDNPLL
                     PNYKWVPIGYHGRSSSIGVSGQRFPRPWGQIKGEGDEPVFAPSRRMDYELELGFFVGP
                     PNESGTVLDMAQAEASLFGATLLNDWSARDVQAWEYQPLGPFLAKNFATTISPWIVTM
                     EALAPFRVPFVRAAGDPQPLPYLDSPANRERGAVAVTLEVWLQTHGMRARGQSAQRIS
                     TSSFASAYWTPAQMLAHHASNGCNLQPGDLLGTGTQSGPNPGEGGSLLELSLGGRQPF
                     ALPDGEQRSFLEDGDCVVLRGHCSAPGARRIGFGDCAGTVLPAIERG"
BASE COUNT          440 a         1287 c         1210 g          460 t
ORIGIN      
        1 gggcgcgagc gcggcctccg cggcgagcgc ctcgcgcacc gcctcgtccg ggccccaggc
       61 agcgaacagc cggcccgagg ccgtgccctc gatgctcatc accgtgccgt ggcgcatcgt
      121 cacgtgaacc ggcgagggcg cttccgcgac acgcaccagc gtcgggccgc ggtggcccca
      181 caccgcgatc gccaccgtgt ggccggtggc gcgggcaagc tcgtccagcc gcacggtggc
      241 caggcggacg ggatcgtact gctgcaggcc gatcaggccc atctgcagcg cgagcggccc
      301 gaggccgtag cggccgccct cggtctcgcg ctcgaccagg cccagcttga tgaagctcac
      361 gaggtagggg tgcgccttcc cggggctcat cgcggccgcc tgggccaggt ccttcagcgc
      421 gagcggctgc ccgtggtgcg ccagcgccag cagcagccgg ccgccgacct cgatcgtctg
      481 gatgccgcgc ggctcggact cgcgggcgcg cgcggcggtg ttcgcgggag tggcggcagg
      541 cggggcggcg ttcttcggca cgggcggcgg cagggtcgtt ggacagcgcg gggcgaggtg
      601 cattatgatg gcgaagcgat tcagcattga tgaatcgatt cagcaaaggt gaaatcgatg
      661 gagacctcgt cctcgaccgc cgcgggcaac gacgcgctgc acgccggctt cggcaaccac
      721 ttcagcagcg aggccgtccc gggcgcgctg ccgcagggcc gcaacagccc gcagcgcgcg
      781 ccgatggggc tgtacgcgga gctgctgtcg ggagccgcct tcaccgcgcc gcgccatgcc
      841 aacgtgcgta cgtggcttta tcgccgccag ccctcggtgc gctgcggtcc gtaccaggcg
      901 ctggagcatc cgctgctcag gaccggcgcg cgcgacggcg tcgacgtggc gcccgatccg
      961 ctgcgctggc atccgcagcc cgtggccgcc ggcgcggggc gcgacttcgt cgacggcctg
     1021 cgcaccatgg tcgtcaacgg cgaccccgac atgcaggtcg gcgtcgccgt ccacctctac
     1081 ggcttcgacc ggccgatggg cgagcgcgcc ttcgcgtgcg ccgacggcga gttgctgctc
     1141 gtcccgcagg acggcgccct gcgcgtcacg acggagctgg gcgtgctgca cgcgggcccg
     1201 ggcgagatcg tgctgctgcc gcgcggcctc gtcttcaagg tcgatccggc cgtcgacggc
     1261 gcggcctgca acggctacgc gtgcgagaac tacggcgccg cgttccgcct tcccgagctg
     1321 ggcccgatcg gctccaacgg cctggccaac acgcgcgact tcatcgcccc gcgcgccgcc
     1381 ttcgacgacg gccccgcgcg cccgtggacg ctggtgcgca agttcggcgg ccggctctgg
     1441 caggcgccgg cgcaggcctc gccgttcaac gtcgtcgcct ggcacggcaa cctcgcgccg
     1501 tacgtctacg acacgcgccg cttcaacacg atcggctcgg tcagcttcga ccatcccgac
     1561 ccgtcgatct tcacggtgct gacctcgccc tcggacacgc cgggctgggc caacgccgac
     1621 ttcgtgatct tcccgccgcg ctggatggtc gccgaggaca ccttccgccc gccctggtac
     1681 caccgcaacg tgatgagcga gtgcatgggc ctcgtctacg gccagtacga cgccaaggag
     1741 gaaggcttcc ggccgggcgg cctgtcgctg cacaacagca tggtgccgca tggcccggac
     1801 gccgacgcgt tcgacaaggc gaccagcgcc gccctcgcgc cgcgcaagct cgacaacacg
     1861 ctcgcgttca tggtcgagag ccgctggcgc ctgcgcccga ccgcgttcgc gctgcagggc
     1921 ggcgcgctgg acgcgtcgta cgccgattgc tgggcgccgc tgcgcgacca cttcacgccg
     1981 gggaactgag atgctcgact tcacccacga cccgcaggcc cgcagctggg tcgactccgc
     2041 ggaggcgccc gacgccgact tcccgctgca gaacctgccg ttcgggcgct tccgccgcgc
     2101 cgcgacgaag gaggctgcgc gggtcggcgt cgccatcggc gaccgcatcc tggacctcgc
     2161 ggcgctcggc gagctcgatg cccaggtcgc gccgctgctc gggccgctgc tgcacggcga
     2221 cctgaacgcc ttcatggcgc agggccgcgc ggcgcggatc gcgctgcggc acgcgctctt
     2281 cgcggggctg tcggccgagc cctcgggcac ggcctcgctg tggcaggcgc ggcgcgacac
     2341 gctgctcgtg ccgcaggacg aggcggagat ggcgctgccc tgccgcatcg gcgactacac
     2401 cgacttctac accggcatcc accacgcgac gacggtgggg cggctgttcc gtcccgacaa
     2461 cccgctgctg ccgaactaca agtgggtgcc gatcggctac cacgggcgca gctcgtcgat
     2521 cggcgtcagc gggcagcgct tcccgcgtcc gtggggccag atcaagggcg agggcgacga
     2581 acccgtcttc gcgcccagcc gccggatgga ctacgagctc gagctcggct tcttcgtcgg
     2641 tccgcccaac gagtccggca cggtgctgga catggcgcag gccgaggcgt cgctgttcgg
     2701 ggcgacgctg ctcaacgact ggtccgcgcg cgacgtgcag gcctgggagt accagccgct
     2761 ggggcccttc ctcgcgaaga acttcgccac cacgatctcg ccctggatcg tcacgatgga
     2821 ggcgctcgcc ccgtttcgcg tgccgttcgt gcgcgcggcc ggcgatccgc agccgctgcc
     2881 ctacctggac tcgcccgcca accgcgagcg cggcgccgtc gcggtcacgc tggaggtctg
     2941 gctgcagacg cacggcatgc gcgcgcgcgg gcaatcggcg cagcgcatct cgaccagctc
     3001 gttcgcaagc gcctactgga cgccggccca gatgctcgcc caccacgcga gcaacggctg
     3061 caacctgcag cccggcgacc tgctcggcac cggcacgcag agcggcccga acccgggcga
     3121 gggcggctcg ctgctggagc tgagcctggg cggacgccag cccttcgcgc tgcccgacgg
     3181 cgagcagcgc agcttcctcg aggatggcga ctgcgtcgtc ctgcgcggcc actgcagcgc
     3241 ccccggcgcg cgtcgcatcg gcttcggcga ctgcgccggc acggtgctgc cggcgatcga
     3301 gcgcggctga gctcctggcc gggcacgagc cccgtgctgg cagggccgac ccacccgtcg
     3361 cgccgaccgc gggcgggcgg gcgcctcgtc gccgtca
//
LOCUS       DASVLN010000888         3905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_531949, whole genome shotgun sequence.
ACCESSION   DASVLN010000888 DASVLN010000000
VERSION     DASVLN010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531949"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF453_20900"
     CDS             complement(<1..252)
                     /locus_tag="VF453_20900"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX7690177.1"
                     /translation="MSSTAAAVDDARLADLIARCALGDRVAFRMLYDATAARLLGVIA
                     QLVGRGAQAEDVLQEVYVRIWKSAGQYRPAAGAPMAWMAA"
     gene            582..1583
                     /locus_tag="VF453_20905"
     CDS             582..1583
                     /locus_tag="VF453_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7690178.1"
                     /translation="MSNPAPGATEPRTPHIPIASTRALYRLDAVSKRLEKLQQQQEHE
                     TLRTTYERMLEKGADRFQVKPAGLPAMEHLYDELPNFREALDDVRRQIALCQDSRDAL
                     EIMPILLLGPPGVGKTHFAREVAQLLGTGMGFVSMSSLTAGWVLSGASSQWKGARPGK
                     VFETLVDGEYANPVMVVDEIDKARAEHAYDPLGSLYSLMEHDTAAAFVDEFAEVPIDA
                     SQVIWIATANDERAIPDPILNRMNVYQVQAPDHDAALAIARRLYAEIRADHDWGRRFD
                     EAAREDVLERLAELAPREMRRALMTGFGNARLAGQGEIRLDDLPAAVARRSRIGFMH"
     gene            1609..2526
                     /locus_tag="VF453_20910"
     CDS             1609..2526
                     /locus_tag="VF453_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEX7690179.1"
                     /translation="MPATLDGRLVVAISSRALFDFEEENRVFEGADDGAYMKLQVERL
                     DAPARPGVAFSLVQKLLRFRDPAPHVEVVILSRNDPMSGLRVFRSAKHYGLPIERGVF
                     TRGRPPWRYLKPLRAHLFLSANAEDVQSALAAGVPAATVATHSVRASGSYPDEVRIAF
                     DGDAVLFSDESERVYQAQGLDAFQAHELARVATPLAPGPFKPLLEALHRLQQASTGGM
                     SIRTALVTARSAPAHERAVLTLRDWKIDVDEAMFLGGLPKGQFLREFEPDFFFDDQTG
                     HVESAASHVPAGHVAAGIANLVAEADAAG"
     gene            2692..3858
                     /locus_tag="VF453_20915"
     CDS             2692..3858
                     /locus_tag="VF453_20915"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7690180.1"
                     /translation="MPRTTSARARASRRQLATLVLLAAAGLAACDRDQPRELRIGVVV
                     AQSGSSAARGKDLVDGARLAADEINAAIYKVDGHPLVVKIDARDDRGDNEAAKQAALA
                     AIDAGASAIIGPLNTPQAAVAIPAVADKGVPELMTATGASLVGLGKGNVLRLLANDDR
                     QGRAMAVFAQEISPGKRIATLAEKTEYGRGLAHSFDDTLKEKGVTPVLALELDDGPLP
                     ADLPARLKAAGVETIALFAREPQLNALLAALEAAGETDVTVLGTNVVRNKSVALRPVR
                     IKALYATATAIDAKEFPEGKAFLQRFGARFGGDPVWGAQIAYDAVYALADAARQARSV
                     DASDLLAALKRIEPNTRVNQQMRFAPSGEQVYPNVAVYKVERGAWQLQMMSANW"
BASE COUNT          575 a         1463 c         1384 g          483 t
ORIGIN      
        1 ggccgccatc caggccatcg gcgcgcccgc ggccggccgg tactggccgg cggacttcca
       61 gatgcgcacg tagacctcct gcagcacgtc ctcggcctgc gcgccgcgcc ccaccagctg
      121 cgcgatgacg cccagcagcc gcgcggcggt ggcgtcgtag agcatgcgga acgcgacgcg
      181 gtcgcccagc gcgcagcgcg cgatgaggtc ggccaggcgg gcgtcgtcga cggcggcggc
      241 cgtggagctc atggggcgat cgctttcaag atggcgcttc ctcgggatgc gccgcatgtg
      301 cggcaggcgg gcaaagggta cgggcaggct gccgatccgg caagcgcggg gagggcgcca
      361 gccggcgcca tgcccccgga tgactggact acgcgcgcgc cgtccgttcg gatgcgcacg
      421 gcccagccga gccatggcag acttcacgcg acagcggcgc gccgatccgg cacagcgctt
      481 gctgagccgc aaatgagggc ggctcgcggc gctgttcgag ggctcgatcc gccgccacct
      541 tgcttcaatg gatgcacgag tcgccgcaaa cggaggtcgc cttgtcgaac ccagcccccg
      601 gggccaccga gccccgcacg ccgcacattc ccatcgcgag cacgcgcgcg ctgtaccggc
      661 tcgatgcggt cagcaagcgc ctcgagaagc tgcagcagca acaggagcac gagacgctgc
      721 gcaccaccta cgagcgcatg ctcgagaaag gcgcggatcg cttccaggtc aagccggcgg
      781 gcctgccggc gatggagcac ctctacgacg agctgcccaa cttccgcgag gcgctggacg
      841 atgtccgccg gcagatcgcc ctgtgccagg acagccgcga cgcgctcgag atcatgccca
      901 tcctgctgct cggccccccg ggcgtcggca agacgcactt cgcgcgcgag gtcgcgcagc
      961 tgctcggcac gggcatgggc ttcgtctcca tgagctcgct gaccgccggc tgggtcctca
     1021 gcggcgcgtc gagccagtgg aagggcgcgc gcccgggcaa ggtcttcgag acgctggtcg
     1081 acggcgagta cgcgaacccg gtgatggtgg tcgacgagat cgacaaggcg cgcgccgagc
     1141 acgcgtacga cccgctgggc tcgctctact cgctgatgga gcacgacacc gccgccgcct
     1201 tcgtcgacga gttcgccgag gtgccgatcg acgcgagcca ggtgatctgg atcgccaccg
     1261 ccaacgacga gcgcgcgatc cccgatccga tcctcaaccg catgaacgtc taccaggtgc
     1321 aggcgcccga ccacgacgcc gcgctggcga tcgcccgccg gctgtacgcg gagatccgcg
     1381 ccgaccacga ctggggccgg cgcttcgacg aagcggcgcg cgaggacgtg ctcgaacgcc
     1441 tggccgagct cgccccgcgc gagatgcggc gcgcgctgat gaccggcttc ggcaacgcgc
     1501 gcctggccgg ccagggcgag atccgcctgg acgacctgcc ggccgcggtg gcgcgacgct
     1561 cgcgcatcgg cttcatgcac tgacgccgcc ggcgactatc atcgggcgat gcccgccacc
     1621 ctggacggcc gcctggtcgt cgccatctcg tcgcgcgcgc tgttcgactt cgaggaggag
     1681 aaccgcgtct tcgagggcgc cgacgacggc gcctacatga agctgcaggt cgaacgcctg
     1741 gacgcgcccg cgcgcccggg cgtcgcgttc tcgctcgtcc agaagctgct gcgcttccgc
     1801 gatccggcgc cgcacgtcga ggtcgtcatc ctctcgcgca acgacccgat gtcgggcctg
     1861 cgcgtgttcc gctcggccaa gcactacggg ctgccgatcg agcgcggcgt gttcacgcgc
     1921 gggcgtccgc cgtggcgcta cctcaagccg ctgcgggcgc acctgttcct cagcgcgaac
     1981 gccgaggacg tccagtccgc gctggccgcg ggcgtgccgg ccgcgacggt ggcgacgcac
     2041 tcggtgcggg cctccggctc gtacccggac gaggtgcgga tcgcgttcga cggcgacgcc
     2101 gtgctgttct ccgacgagtc cgagcgggtc taccaggcgc aggggctgga cgccttccag
     2161 gcgcacgagc tcgcgcgcgt cgcgacgccg ctggcgcccg ggccgttcaa gccgttgctc
     2221 gaggcgctgc accggctgca gcaggcgtcg acgggcggca tgtcgatccg caccgcgctc
     2281 gtcaccgcac gcagcgcgcc ggcgcacgag cgcgccgtgc tgacgctgcg cgactggaag
     2341 atcgacgtcg acgaggcgat gttcctcggc ggcctgccca agggccagtt cctgcgcgag
     2401 ttcgagcccg acttcttctt cgacgaccag accggccacg tcgaaagcgc cgccagccac
     2461 gtgccggccg gccacgtcgc cgcgggcatc gccaacctcg tggccgaggc ggacgccgcg
     2521 ggctgagcga atccgcggcc ccgccgtggc gtgaagattc accgccggcg cattgcggga
     2581 atccacatat cgaaccgcgg tcccttgccg gcatggcctg cgcggcagca gagtcgagtc
     2641 cccgccagac aggccccgcg caccgcgaag gggccgcagg agacaagcga catgccccga
     2701 acgacctccg cccgcgcccg cgcctcgcgg cgccagctcg cgaccctcgt cctcctcgcc
     2761 gccgccggcc tcgccgcctg cgaccgcgac cagccgcgcg agctgcgcat cggcgtcgtc
     2821 gtcgcccaga gcggcagctc cgccgcgcgc ggcaaggatc tggtcgacgg cgcccgcctg
     2881 gccgccgacg agatcaacgc cgccatctac aaggtcgacg gccatccgct ggtcgtgaag
     2941 atcgacgccc gcgacgaccg cggcgacaac gaggccgcga agcaggccgc gctcgccgcg
     3001 atcgacgccg gcgcgagcgc catcatcggg ccgctgaaca cgccccaggc cgcggtcgcg
     3061 atcccggcgg tcgccgacaa gggcgtcccc gagctgatga cggccaccgg cgcctcgctc
     3121 gtcggcctgg gcaagggcaa cgtgctgcgc ctgctcgcca acgacgaccg ccagggccgc
     3181 gcgatggccg tcttcgcgca ggagatctcg ccgggcaagc gcatcgcaac gctggccgag
     3241 aagaccgagt acggccgcgg actcgcgcac agcttcgacg acacgctcaa ggagaagggc
     3301 gtcacgccgg tgctggccct ggagctcgac gacggcccgt tgcccgccga cctgccggcc
     3361 cggctgaagg cggccggcgt cgagaccatc gcgctgttcg cgcgcgagcc gcagctgaac
     3421 gcgctgctcg cggcgctcga ggccgccggc gagaccgacg tcaccgtgct cggaacgaat
     3481 gtcgtgcgca acaagtcggt cgcgctgcgg ccggtgcgga tcaaggcgct gtacgcgacc
     3541 gccaccgcga tcgacgcgaa ggaattcccc gagggcaagg ccttcctcca gcgcttcggc
     3601 gcgcgcttcg gcggcgatcc ggtgtggggc gcgcagatcg cgtacgacgc ggtgtacgcg
     3661 ctcgccgacg ccgcccgcca ggcgcgcagc gtcgacgcgt ccgacctgct cgcggcgctc
     3721 aagcgcatcg agccgaacac ccgggtgaac cagcagatgc gcttcgcgcc ctcgggcgag
     3781 caggtctacc cgaacgtcgc cgtctacaag gtcgagcgcg gcgcgtggca gctgcagatg
     3841 atgtcggcga actggtgatg gcgcggcgcc ggccgcgcgc gccggctcag gcgagcgcgg
     3901 ccggg
//
LOCUS       DASVLN010000889         1781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_369325, whole genome shotgun sequence.
ACCESSION   DASVLN010000889 DASVLN010000000
VERSION     DASVLN010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369325"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VF453_20920"
     CDS             <1..986
                     /locus_tag="VF453_20920"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX7690181.1"
                     /translation="DTPTASGQLLPVRPAGAAAALPPYVFAVGDEFDLRVPDAPQFDQ
                     TLKVRPDGKVSLALVGSVYAQGRTPEDVEGEIRERLHALGGAPGAREYLLQPNDEIEV
                     KFPYTPALNEAQRIRPDGKIQMQEIGTVVAEGLSPEELQQRLKAAYARVLRHPELSVI
                     VRSVTTQNVRVSTAAGPVTARAGLGALDPTLIVRTTQAPQVFVTGEVGKPGVLAWRPG
                     FSLVQALAEAGGALPTGDGAHIVVLRRGAGAPGQPDGVQVLRLDLPRDFARQPDRDVA
                     LQPFDVVLLPRSDIAVMGDKLNAYVLNLLPPLKNSSFGFVYDLSSSNSIIR"
     gene            1021..>1781
                     /locus_tag="VF453_20925"
     CDS             1021..>1781
                     /locus_tag="VF453_20925"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX7690182.1"
                     /translation="MQGPSSTLFPPGQPSSAPPRSLRDLVAALYRQAGLVAICALVGT
                     AASLALYARQPRVYTSSAKVWVQTEQQGSPSFLSGIAAYRETPYPDPVNRKIETEIEL
                     MLTRTNAQAVVEKLGIRDDQLARGGASRPHPGATPGDERAHTVDLFLDGLAVEPLRSK
                     TADTSSNVLEVKFECTDRALAPVALQAFLDNYLQLGAQQNRRLGETTSRVIETQLAQA
                     KDELRQAEDGIVALLVRDGGRSARPADAAALAAGGS"
BASE COUNT          250 a          679 c          633 g          219 t
ORIGIN      
        1 ccgacacgcc caccgcgagc ggccaactgc tgcccgtgcg cccggcgggc gccgccgccg
       61 cactgccgcc gtacgtgttc gcggtcggcg acgagttcga cctgcgcgtg cccgacgcgc
      121 cgcagttcga ccagacgctc aaggtgcgcc ccgacggcaa ggtgagcctc gcgctggtgg
      181 gcagcgtcta cgcgcagggc cgcacgcccg aggacgtcga gggcgagatc cgcgagcgcc
      241 tgcacgcgct cggcggcgcc ccgggcgcgc gcgagtacct gctgcagccc aacgacgaga
      301 tcgaggtcaa gttcccgtac acgccggcgc tcaacgaggc gcagcgcatc cgccccgacg
      361 gcaagatcca gatgcaggag atcggcaccg tcgtcgccga gggcctgagc cccgaggagc
      421 tgcagcagcg cctgaaggcc gcctacgcgc gcgtgctgcg ccatcccgag ctctcggtga
      481 tcgtgcgcag cgtcacgacg cagaacgtgc gcgtgagcac cgccgcgggc ccggtcacgg
      541 cgcgtgcggg cctgggcgcg ctggacccga ccctcatcgt gcgcaccacg caggcgccgc
      601 aggtcttcgt gacgggcgag gtcggcaagc cgggcgtgct cgcgtggcgc ccgggcttca
      661 gcctcgtgca ggcgctggcc gaggccggcg gcgcgctgcc caccggggac ggcgcgcaca
      721 tcgtcgtgct gcgccgcggc gccggcgcac ccggccagcc ggacggggtg caggtgctgc
      781 gcctggacct gcccagggac ttcgcgcgcc agcccgatcg cgacgtcgcg ctgcagccgt
      841 tcgacgtcgt gctgctgccg cgctccgaca tcgcggtgat gggcgacaag ctcaacgcgt
      901 acgtcctcaa cctgctgccg ccgctgaaga acagctcctt cggcttcgtg tacgacctga
      961 gctcgagcaa ctcgatcatc cgctgatccc ggccgatccg cacgacgccc gaaagacgcc
     1021 atgcaaggac cttcctccac cttgttcccc ccggggcagc cgagctcggc gccgccgcgc
     1081 agcctgcgcg acctcgtcgc cgcgctgtac cgccaggcgg ggctggtcgc gatctgcgcg
     1141 ctggtcggca ccgcggcctc gctcgcgctg tacgcgcgcc agccgcgcgt ctacacctcc
     1201 agcgcgaaag tctgggtgca gaccgagcag cagggctcgc cgtccttcct ctcgggcatc
     1261 gcggcctacc gcgagacgcc gtatcccgat ccggtcaacc gcaagatcga gaccgagatc
     1321 gagctgatgc tcacgcgcac caacgcgcag gcggtggtcg agaagctcgg catccgcgac
     1381 gaccagctcg cccgcggcgg cgcgtcccgg ccgcatcccg gggcgacgcc gggcgacgag
     1441 cgcgcgcaca ccgtcgacct gttcctggac ggcctggcgg tcgagccgct gcgctcgaag
     1501 acggcggaca cgtcgtccaa cgtgctggag gtgaagttcg agtgcaccga ccgcgccctc
     1561 gcgccggtgg cgctgcaggc cttcctcgac aactacctgc agctcggcgc gcagcagaac
     1621 cgccgcctgg gcgagacgac ctcgcgcgtg atcgagaccc agctcgcgca ggccaaggac
     1681 gagctgcgcc aggcggagga tggcatcgtc gcgctgctgg tgcgcgacgg cggtcgcagc
     1741 gcgcggccgg ccgacgcggc ggcgctggcc gcgggcgggt c
//
LOCUS       DASVLN010000890         5945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_477636, whole genome shotgun sequence.
ACCESSION   DASVLN010000890 DASVLN010000000
VERSION     DASVLN010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477636"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VF453_20930"
     CDS             <1..515
                     /locus_tag="VF453_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690183.1"
                     /translation="TAYGPVRPLVGVLFTDTVGDNRATFSNPDGSTASGNPSRSAEVL
                     AGAEFALAANGLRLRLAAGLQASNLSSGGGHERFTRVPLEATLMYPVGEQLRVGGGLR
                     YAARLRFGGAGGGTSDQLNATPGVIGAVDYRLSPHLLADFRYVYERYEVSGSGDYEAS
                     HWGVGLTAMY"
     gene            676..1284
                     /locus_tag="VF453_20935"
     CDS             676..1284
                     /locus_tag="VF453_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690184.1"
                     /translation="MKHARRAAAAAAAAIALLCPVLACAQADMAPPGAVAIHPVIGGG
                     LTAGGDKLLAVRYTDGDTRTLRAGQLLQVWAGLDVRPVDSPVSFLATVGYHSDSAGGW
                     NGSVRFERFPIEASVLWSPAPAFRIGVGARYAAGAAVRSTGVVDDIPNPHFKSRLGGL
                     VLAEWLVTPHQGVQLRYVHETYRLSGQSIDGSHGGLGYSFYF"
     gene            1515..2108
                     /locus_tag="VF453_20940"
     CDS             1515..2108
                     /locus_tag="VF453_20940"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7690185.1"
                     /translation="MRKILACAALGAALLAPAFAHAASDDSGLHPVIGMLLTGGGSKT
                     VQVQYTDGTGYTMRAGQLFQLYGGAEYRVPDSGVAVQVSIGYHFDSAGGDNGDVRFTR
                     YPIEALVLYQLAPQWRLGAGVRDATNAKLHGSGAGEGIDAKFDSSIGAIVQAEWLPSP
                     RWGVQLRYVHETYKLKDATDDPRISGNHVGLGTAYYF"
     gene            complement(2215..4806)
                     /locus_tag="VF453_20945"
     CDS             complement(2215..4806)
                     /locus_tag="VF453_20945"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEX7690186.1"
                     /translation="MTKSLIIAEKPSVAQDIVRALTPTSGKFEKAAEYFENDRYVVTS
                     AVGHLVEIKAPEEYDVKRGKWSFANLPVVPPHFDLAPIEKAKTRLNAVVKLVRRKDIT
                     ELINACDAGREGELIFRLIVQFAEPARGKLDKPIRRLWLQSMTPQAIRDGFEQLRSDQ
                     QMQGLASAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIVVEREE
                     KIRKHVPRDYWEIRATFDAQAGQYDAKWVDPGFKKGDDADARADRVWDKAKAEAIARA
                     VLGQPGAVTEESKPSTSASPLLYDLTSLQREANGRFGFSAKTTLSLAQALYEKHKVLT
                     YPRTDARALPEDYLPVVKDTLKMLATEDLPGPLRELSPHAAKALKESYVKPTKRVFDN
                     SKISDHFAIIPTLQAPKSLSEAEAKLYDLVVKRFIAVFFPSAEYQVTTRLTQVGEHRF
                     QTNGKVLVNPGWLAVYGKEAQDEDATLVPVAKGETVRNESVEAVGLKTRPPARYNEAT
                     LLSAMEGAGKMIDDEELREAMSEKGLGTPATRAQIIEGLINEKYMLREGRDLIPTAKA
                     FQLMTLLRGLGVEDLTRPELTGNWEFQLSEMEHGRLSRDAFMTDIARMAERIVKKAKE
                     YDRDTIPGDYATLTTPCPNCGGVVKENYRRFTCTGPRGDGTDSCEFSITKIPAGRAFD
                     AAEADAFLRDKHIGPLDGFRSKAGFPFVAELKLAYDDEIRNWKLEFDFGEDAKKAEGE
                     GEPVSFEGQESLGACPKCKGRVFEFGNSYVCEHAVGAHVTCDFKSGKIILQQPVSHEQ
                     MHKLLATGKTDLLENFVSNKTRRKFKAFLAYDKKEGKVNFEFEPRAAKAPAAKKTAAR
                     KKADAEE"
     gene            4915..5400
                     /locus_tag="VF453_20950"
     CDS             4915..5400
                     /locus_tag="VF453_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX7690187.1"
                     /translation="MTSKLQAQNHTAPGGRRIQVRRSGVHGKGVFAARDLARGEVLIE
                     YIGERITWEEALDRHPHDPLQPDHTFYFHLTDDCVIDANVGGNASRWINHACSPNCEA
                     DEVDGRVFIKARKAIKAGEELFYDYGLIIDEPYTKALKKRFECRCGTPRCRGTMLSPK
                     R"
     gene            5409..>5945
                     /locus_tag="VF453_20955"
     CDS             5409..>5945
                     /locus_tag="VF453_20955"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX7690188.1"
                     /translation="MTAPQAAPSRRADAAPEPVHWGAEALWEALVPVLPGLSIEVVER
                     LDSTNSELTERLRRASRVQQDRGGRQDDLFPQLLVATHQTAGRGRLGRRWHSAPGSSL
                     TFSLALALQRADWSGLSLAVGLAVVDALDPHGRHLGLKWPNDLILLDGRAEDGAPRLG
                     RKLGGILIESVAVGSRRAA"
BASE COUNT          795 a         2172 c         2040 g          938 t
ORIGIN      
        1 ggacggccta cgggccggtg cggcccctcg tgggcgtcct gttcacggac accgtcggcg
       61 acaaccgggc gaccttctcc aacccggacg gcagcaccgc cagcggcaac ccgagccgga
      121 gcgccgaagt gctcgcgggc gccgagttcg cgctcgccgc caacggcctg cggctgcgcc
      181 tcgccgcggg gctgcaggcg agcaacctgt ccagcggcgg cggccacgag cgcttcacgc
      241 gcgtgccgct cgaggcgacg ctgatgtatc cggtcggcga gcagctgcgc gtcggcggcg
      301 gcctgcgcta cgccgcgcgc ctgcgcttcg gcggcgcggg cggcggcacc tccgaccagc
      361 tgaacgcgac gcccggcgtc atcggtgccg tggactaccg cctgtcgccg cacctgctcg
      421 ccgacttccg ctacgtctac gagcgctacg aggtctccgg cagcggcgac tacgaggcca
      481 gccactgggg cgtcggcctg acggccatgt actgacgcgg gcgcggcgcg cgtcgtcggt
      541 tttcgcccac ggtcgagcag gccgcgcagg gcgtgctggc tatgatcgac cggctgcggg
      601 gcgggcgtcc cgcccgctcg ccgcacaggg aggaccggca gcgccgcgtc cacgatcacc
      661 gtgagaagga tcgccatgaa acacgcccgt cgcgccgcgg ccgccgccgc cgccgccatc
      721 gccctgctct gtcccgtcct cgcctgcgcg caggccgaca tggcgccgcc cggcgccgtc
      781 gcgatccacc cggtcatcgg cggcgggctg accgccggcg gcgacaagct gctggccgtg
      841 cgctacaccg acggcgacac gcgcacgctg cgcgccggcc agctgctgca ggtctgggcc
      901 ggcctcgacg tgcggccggt ggactcgccg gtctccttcc tcgccaccgt cggctaccac
      961 agcgacagcg ccggcggctg gaacggctcg gttcgcttcg agcgcttccc catcgaggcc
     1021 tccgtgctct ggagccccgc gcccgccttc cggatcggcg tcggcgcgcg ctacgccgcg
     1081 ggcgcggcgg tgcgcagcac gggcgtggtc gacgacatcc cgaacccgca cttcaagtcg
     1141 cgcctgggcg gcctcgttct ggcggagtgg ctcgtcaccc cgcaccaggg cgtgcagctg
     1201 cgctacgtgc acgagaccta ccgcctctcc ggccagtcca tcgacggcag ccacggcggc
     1261 ctcggctaca gcttctactt ctgaagccga cgcggcgcgc ggcgcggccg agcgtcgcgg
     1321 agccgaacgc gaggtttcga tcgcgcggcc cgttgccggc ggatcgtgcg atccgtcacg
     1381 cctgggcggt ggtgccgtcg cgcgaacgcg acgccggtcg cggacgttcc ttcgccgccc
     1441 ggtagcatgc gcgcgccgcg ccaaggcgtc ggggagggct ctgcgccgcc ggcatcacac
     1501 gagaacatcc catcatgagg aagatccttg cctgcgccgc gctcggcgcc gcgctgctcg
     1561 cgcccgcatt cgcccacgcg gcgagcgacg acagcggcct gcaccccgtc atcggcatgc
     1621 tcctgaccgg cggcggctcc aagaccgtgc aggtgcagta caccgacggc accggctaca
     1681 cgatgcgcgc cggccagttg ttccagcttt acggcggcgc ggaataccgc gtgcccgaca
     1741 gcggcgtcgc cgtccaggtc agcatcggct accacttcga ctcggccggc ggcgacaacg
     1801 gcgacgtgcg cttcacgcgc tatccgatcg aggcgctcgt gctgtaccag ctcgcgccgc
     1861 aatggcgcct gggcgcgggc gtgcgcgacg ccaccaacgc caagcttcat ggcagcggcg
     1921 ccggcgaggg catcgacgcg aagttcgact cgtcgatcgg cgcgatcgtg caggccgagt
     1981 ggctgccgag cccgcgctgg ggcgtgcagc tgcgctacgt gcacgagacc tacaagctca
     2041 aggacgccac cgacgacccg cgcatcagcg gcaaccacgt cggcctgggc accgcctact
     2101 acttctgact ccgacgccct gatccggcgc cctggcggcg cgtccgccag cgaaacggcc
     2161 acccgcgggt ggccgttgct catggcgacg cgcgatcgcc gcggccgcgc cggatcactc
     2221 ctcggcgtcc gccttcttgc gagcggcggt cttcttcgcc gccggcgcct tggccgcgcg
     2281 cggctcgaac tcgaagttca ccttgccctc cttcttgtcg taggcgagga aggccttgaa
     2341 cttgcggcgc gtcttgttgg agacgaaatt ctccagcagg tcggtcttgc cggtggccag
     2401 cagcttgtgc atctgctcgt gcgagacggg ctgctgcagg atgatcttgc cgctcttgaa
     2461 gtcgcaggtg acgtgcgcgc ccaccgcatg ctcgcagacg taggagttgc cgaactcgaa
     2521 cacgcgtccc ttgcacttcg ggcacgcgcc gagcgattcc tggccctcga agctcaccgg
     2581 ctcgccctcg ccctcggcct tcttcgcgtc ctcgccgaag tcgaattcga gcttccagtt
     2641 gcggatctcg tcgtcgtagg cgagcttgag ctccgccacg aacgggaagc ccgccttgga
     2701 gcggaagccg tccagcggcc cgatgtgctt gtcgcgcagg aaggcatcgg cctccgcggc
     2761 gtcgaaggcg cggccggcgg ggatcttggt gatcgagaac tcgcacgagt cggtgccgtc
     2821 gccgcgcggg ccggtgcagg tgaagcggcg gtagttctcc ttgaccacgc cgccgcagtt
     2881 cgggcacggc gtcgtcagcg tcgcgtagtc gccggggatc gtgtcgcggt cgtactcctt
     2941 ggccttcttg acgatgcgct cggccatgcg cgcgatgtcg gtcatgaagg cgtcgcgcga
     3001 aagccggccg tgctccatct ccgacagctg gaactcccag ttgccggtca gttcgggacg
     3061 tgtcaggtcc tcgacgccca ggccccggag cagcgtcatc agctggaacg ccttggccgt
     3121 cgggatgagg tcgcggccct cgcgcagcat gtacttctcg ttgatgaggc cttcgatgat
     3181 ctgcgcccgc gtcgccggcg tgcccaggcc cttttcggac atggcctcac gcagctcctc
     3241 gtcgtcgatc atcttgcccg cgccttccat cgcggacagc agcgtcgcct cgttgtagcg
     3301 cgccggcggg cgcgtcttca ggcccacggc ctcgaccgac tcgttgcgca ccgtctcgcc
     3361 cttggccacc ggcacgaggg tggcgtcctc gtcctgcgcc tccttgccgt agacggccag
     3421 ccagcccggg ttcacgagca ccttgccgtt ggtctggaag cggtgctcgc cgacctgcgt
     3481 caggcgcgtc gtgacctggt attcggcgct cgggaagaac acggcgatga agcgcttgac
     3541 gacgaggtcg tagagcttgg cctcggcctc cgagagcgac ttgggcgcct gcagcgtcgg
     3601 gatgatcgcg aagtggtccg agatcttcga gttgtcgaag acgcgcttgg tcggcttgac
     3661 gtagctctcc ttgagcgcct tggccgcatg cggcgagagc tcgcgcagcg ggcccggcag
     3721 gtcctcggtc gcgagcatct tcagcgtgtc cttgacgacc ggcaggtagt cctcgggcag
     3781 cgcgcgcgcg tccgtccgcg ggtaggtgag caccttgtgc ttctcgtaga gcgcctgtgc
     3841 gagcgacagc gtcgtcttcg ccgagaaccc gaagcggccg ttggcctcgc gctgcaggct
     3901 ggtgaggtcg tacagcagcg ggctcgcgga cgtgctcggc ttcgattcct ccgtcacggc
     3961 gccgggctgg cccagcaccg cgcgcgcgat cgcctcggcc ttcgccttgt cccagacgcg
     4021 gtcggcgcgc gcgtcggcgt cgtcgccctt cttgaacccc gggtcgaccc acttggcgtc
     4081 gtactggccg gcctgggcgt cgaaggtggc gcggatctcc cagtagtcgc gcggcacgtg
     4141 cttgcggatc ttctcctcgc gctcgacgac gatggacagc gtcggcgtct gcacgcgccc
     4201 gaccgtcgtg aggaagaagc cgccgtcgcg cgagttgaac gcggtcatcg cgcgggtgcc
     4261 gttgatgccg accagccagt cggcctccga gcggctgcgc gcggccgacg cgagaccctg
     4321 catctgctgg tcgctgcgca gctgctcgaa gccgtcgcgg atcgcctgcg gcgtcatgct
     4381 ctgcagccac aggcggcgga tcggcttgtc gagcttgccg cgcgcgggct cggcgaactg
     4441 cacgatgagt cggaagatca gctcgccctc gcgccccgcg tcgcaggcgt tgatcagctc
     4501 ggtgatgtcc ttgcgccgca cgagcttgac gaccgcgttc agacgcgtct tggccttctc
     4561 gatgggcgcg aggtcgaagt gcggcggcac gaccggcagg ttggcgaagc tccacttgcc
     4621 gcgcttgacg tcgtactcct cgggggcctt gatctcgacc aggtggccca cggccgacgt
     4681 gacgacatag cggtcgttct cgaagtactc ggcggccttc tcgaacttgc ccgaggtcgg
     4741 cgtgagcgcc cggacgatgt cctgcgccac gctgggcttt tcggcgatga tcagtgactt
     4801 tgtcatttat ttaccttgaa ccgggcagga cactccgaag ccctgcctaa aatgcgtctc
     4861 tcgcgcgcgc ccgcgctcac atacgcgcgc gcgcccacaa tttcaatgta ccgaatgacg
     4921 tcgaagctgc aagcgcaaaa ccataccgcc ccgggcggcc gccggatcca ggtgcgacgc
     4981 agcggcgtcc acggcaaggg cgtcttcgcc gcccgcgacc tcgcgcgcgg cgaggtcctg
     5041 atcgagtaca tcggcgagcg catcacctgg gaggaggcgc tcgaccgcca tccccacgac
     5101 ccgttgcagc ccgaccacac gttctatttc cacctcacgg acgactgcgt catcgacgcc
     5161 aacgtcggcg gcaacgcctc gcgctggatc aaccacgcct gctcgcccaa ctgcgaggcc
     5221 gacgaggtcg acggccgcgt gttcatcaag gcgcgcaagg cgatcaaggc gggcgaggag
     5281 ctgttctacg actacggcct gatcatcgac gagccgtaca cgaaggcgct gaagaagcgc
     5341 ttcgaatgcc gctgcggcac accccgctgc cgcggcacca tgctgtcgcc caagcgctga
     5401 cgtcccccgt gaccgccccg caggccgccc cgtcccgccg cgccgacgcc gcgccggagc
     5461 ccgtgcactg gggcgccgag gcgctctggg aggcgctggt gcccgtgctg cccgggctgt
     5521 cgatcgaggt ggtcgagcgg ctcgactcca ccaacagcga actcacggag cgcctgcgcc
     5581 gcgcgtcgcg cgtgcagcag gatcgcggcg gccgccagga cgacctgttc ccgcaactgc
     5641 tggtcgcgac gcaccagacc gccggccgcg ggcgcctggg acggcgctgg cactcggcgc
     5701 cgggcagctc gctcacgttc tcgctcgcgc tggcattgca gcgggcggac tggtcggggc
     5761 tgtcgctggc ggtcgggctc gccgtcgtcg acgcgctcga cccgcacggc cgccacctcg
     5821 gcctgaagtg gcccaacgac ctcatcctgc tcgacggccg cgccgaggac ggcgcgccgc
     5881 gcctgggccg caagctcggc ggcatcctga tcgagtccgt ggcggtcggc agccggcgcg
     5941 cggcc
//
LOCUS       DASVLN010000891         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_477646, whole genome shotgun sequence.
ACCESSION   DASVLN010000891 DASVLN010000000
VERSION     DASVLN010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477646"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF453_20960"
     CDS             <1..204
                     /locus_tag="VF453_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX7690189.1"
                     /translation="AFGLPDGAATLLTWGGLRGGLSLAMALALPAGVSRDVLGALAYA
                     VVAFAILVQGSTFARLVRGGGRR"
     gene            256..636
                     /locus_tag="VF453_20965"
     CDS             256..636
                     /locus_tag="VF453_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690190.1"
                     /translation="MDLRVRIKITDELSEDVIESTGELDLASGEIRNVEYEDWDVEAQ
                     GQPVHAEDYEFTVGTLSNNGKDVEFKVDVNRATGAYSVSPSELLEIKVRAAQLFAGLS
                     GKDMAARGPGPKATPAGKGRGRLH"
     gene            complement(732..881)
                     /locus_tag="VF453_20970"
     CDS             complement(732..881)
                     /locus_tag="VF453_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690191.1"
                     /translation="MKTKSKIVRWGTLAILVAAMSGCCVAPWGYGGRGYYGHGYYGGG
                     WHRGG"
     gene            1182..1418
                     /locus_tag="VF453_20975"
     CDS             1182..1418
                     /locus_tag="VF453_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690192.1"
                     /translation="MIKHLAILSSAVVAAAALSGCIVAAPPPAYGVAYTPPPGVVYVA
                     PTYAAPGPGWEWRYHATYGWGWWHPQRGWHRGWR"
     gene            1619..2740
                     /locus_tag="VF453_20980"
     CDS             1619..2740
                     /locus_tag="VF453_20980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7690193.1"
                     /translation="MPERDPALSASFAHDGGGDRRVWLTYAGACLLAWLLYVLAGAEF
                     ERGLWQLWEAVYQATLSLWAPMAIGTAALPWVRMLQRRELGGAAAASLHVLAALVFGG
                     LWQLLDFSLAWLMYGPDHARAVLMQTVLWRAIWGAVVYAAVATAFTAMLQTRRARRAA
                     IAAAQAESALARAELAVISGKLNPHFLFNTLNSIIALTRKDAAGAEAALMRFAGMLRY
                     VLDTKRSAADRVPLADEIEFVRDYLALESLRLGARLGIDWEIDPGTMADEIPPLSLQP
                     LVENSIVHGIAPRVRGGRIRIRSARDAAGEGLALSVEDDGPGCDPATLDDPSDAARRG
                     IGLGALRRRFALDYDGRARLQVRTAPGAGFRVDLWIPQT"
     gene            2802..3560
                     /locus_tag="VF453_20985"
     CDS             2802..3560
                     /locus_tag="VF453_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7690194.1"
                     /translation="MTVTERTRVLIAEDEPLAAEALAAWVAEMPQLELAATCGDGESA
                     LAQIRALQPGLVLMDIQMPGLTGLQVLRALADDGGPRPAVIFTTAYDEHALAAFELHA
                     VDYLLKPFARERFVEAVEHALQAGGRGDAAGAAEALERPAGEPMTRVLVRDQGKIFPL
                     QVETIEYLRSDTKYTALACRGRQFLVRLPITTFEQRLDPARFLKLHRSCIVNLDFVES
                     MVPDENSQLVVQMTDGTRITANREVSKRLREQSI"
BASE COUNT          490 a         1365 c         1281 g          504 t
ORIGIN      
        1 gccttcgggc tgcccgacgg cgcggcgacg ctgctcacct ggggcgggct ccgcgggggc
       61 ctgtcgttgg cgatggcgct cgcgctgccg gcgggggtct cgcgcgacgt gctgggcgcg
      121 ctcgcctatg cggtcgtggc cttcgcgatc ctcgtgcaag gttcgacgtt tgcccgtctc
      181 gttcggggcg gcggacgtcg ctgacggcgc gcgcgtgcca gaatcgcgcc ctcaccaaga
      241 acgacgaggg accacgtgga cctgagagta cggatcaaga tcaccgacga gctgagcgag
      301 gacgtcatcg agtccaccgg cgaactcgac ctggcgagcg gcgagatccg caacgtcgag
      361 tacgaagact gggacgtcga ggcccagggc cagcccgtgc acgccgagga ctacgagttc
      421 accgtcggca ccttgtccaa caatggcaag gacgtggagt tcaaggtcga cgtcaaccgt
      481 gcgaccggcg cgtactcggt cagcccgagc gagctgctcg agatcaaggt gcgcgcggcg
      541 cagctgttcg cgggcctgtc gggcaaggac atggccgccc gcggccccgg cccgaaggcg
      601 acgcccgccg gcaagggccg cggccgcctg cattgatcgc gcggccgggc cgcccggaaa
      661 gcgaaaaggc cgcgcgagcg gcctttcgcg tttgcgcacg acgccggcgg gcggcgtcgc
      721 gatcgcgcgg atcagccgcc gcggtgccag ccgccgccgt agtagccgtg gccgtagtag
      781 ccgcggccgc cgtagcccca gggcgccacg cagcagccgc tcatcgcggc caccaggatc
      841 gccagcgtgc cccagcgcac gatcttcgac ttcgtcttca tcatccaact cctgaggccg
      901 caacagcgct gcctgccggt tcaacgtgca ttcagcgggc ccgatgactt gcccgaggtt
      961 tcgcggatgc aaagagacgt aaacaccccg gccgccagcc cctcgtctgc cggatgaagc
     1021 gagcatgcat gcgcccttgc tgctcgacgg caggccgcgg ccgcggcgtc aaccgtcgcg
     1081 cagggcctgc cgttgaacca cgaggggcgc agctggcgcc gcggtcgacg cgacgggcgc
     1141 gcccgttgcc actgtcctca acaacgaatg gatggaacat catgatcaag cacctcgcga
     1201 tcctgtcgag cgccgtcgtg gccgctgccg cgctgtcggg ctgcatcgtc gccgcgccgc
     1261 ctccggcgta tggcgtcgcc tacacgccgc cgccgggggt ggtctacgtc gccccgacct
     1321 acgccgcgcc cggcccgggc tgggaatggc gctaccacgc gacctacggc tgggggtggt
     1381 ggcacccgca gcgcggctgg caccgcggct ggcgctgagc ctcgcgcgct cgtcgcgata
     1441 gctgggccgg ctaaccgaca agtgcagcgt aatggtctcg tgcgactggc cgggaggcgg
     1501 gtcgcgcgcc gcgaacgagc cgccgcgcgc cgacgccccc agccgcattt cgggcgcggc
     1561 atcccgcatt tggtcacctc gcgccggcgc atgccgggcc tgcccgtaac atgtgcccat
     1621 gcccgaacgc gaccccgccc tgtccgcctc cttcgcccac gatggcggag gcgaccggcg
     1681 cgtgtggctc acgtacgccg gcgcctgcct gctggcgtgg ctgctgtacg tgctggccgg
     1741 cgccgagttc gagcgcgggc tgtggcagct ctgggaggcc gtctaccagg cgacgctgag
     1801 cctgtgggcg ccgatggcga tcggcaccgc ggcgctgccg tgggttcgca tgctgcagcg
     1861 gcgcgagctc ggcggcgcgg ccgccgcgtc cctgcacgtg ctggcggcgc tcgtcttcgg
     1921 cgggctttgg cagttgctgg acttctcgct cgcctggctg atgtacggcc ccgaccatgc
     1981 acgcgccgtg ctgatgcaga ccgtgctgtg gcgcgcgatc tggggcgccg tcgtgtacgc
     2041 cgcggtcgcg accgccttca ccgcgatgct gcagacgcgt cgcgcccgcc gcgccgcgat
     2101 cgccgcggcg caggccgagt cggcgctcgc gcgcgccgag ctcgccgtga tcagcggcaa
     2161 gctcaatccg cacttcctgt tcaacacgct caactccatc atcgcgctca cgcgcaagga
     2221 cgccgccggc gccgaggccg cgctgatgcg cttcgcgggc atgctgcgct acgtcctcga
     2281 caccaagcgc agcgccgccg accgcgtgcc gctggccgac gagatcgagt tcgtgcgcga
     2341 ctacctggcg ctcgagagcc tgcgcctggg cgcgcggctg ggcatcgact gggagatcga
     2401 tcccggcacg atggccgacg agatcccgcc gttgagcctg cagccgctgg tcgagaacag
     2461 catcgtccac ggcatcgccc cgcgcgtgcg cggcggtcgc atccgcatcc gcagcgcgcg
     2521 cgacgccgcg ggcgaaggcc tcgcgctgag cgtcgaggac gacggccccg gctgcgaccc
     2581 cgccacgctg gacgacccct ccgacgccgc ccgccgcggc atcgggctgg gcgcgctccg
     2641 gcgccgcttc gccctcgact acgacggccg cgcccggctc caggtgcgca ccgcgcccgg
     2701 ggccggcttt cgcgtcgatc tctggatccc gcagacatga gcgcccgccc ggccgcccca
     2761 ggggcgctcg caccgcaggc cggcaggccg gaggctttcc aatgaccgtc accgaacgca
     2821 cccgcgtcct catcgccgag gacgaaccgc tggccgccga ggcgctcgcc gcctgggtcg
     2881 ccgagatgcc gcagctcgaa ctcgccgcca cctgcggcga cggcgagagc gcgctcgcgc
     2941 agatccgcgc gctgcagccc ggactcgtgc tgatggacat ccagatgccg ggcctgaccg
     3001 ggctgcaggt gctgcgtgcg ctcgccgacg acggcggccc gcgcccggcc gtcatcttca
     3061 cgaccgccta cgacgagcac gcgctcgcag cgttcgagct gcacgccgtc gactacctgc
     3121 tcaagccctt cgcgcgcgag cgcttcgtcg aggccgtgga gcacgcgctg caggccggcg
     3181 gacgcggtga cgcggcgggc gcggccgagg cgctcgagcg ccccgcgggc gagccgatga
     3241 cgcgcgtgct ggtgcgcgac cagggcaaga tcttcccgct gcaggtcgag acgatcgagt
     3301 acctgcgctc cgacaccaag tacacggcgc tcgcctgccg cgggcgccag ttcctggtgc
     3361 gcctgccgat caccaccttc gagcagcggc tcgaccccgc gcgcttcctc aagctgcacc
     3421 gcagctgcat cgtcaacctg gatttcgtcg agtcgatggt gcccgacgag aactcgcagc
     3481 tggtcgtgca gatgacggac ggcacgcgca tcacggccaa ccgcgaggtc tccaagcggc
     3541 ttcgggagca atcgatatga gcacgccgcg cgcctcgcgc gccatttcgc gacccttcgc
     3601 tcgcgccgtc gcggtcgcgg cgctcgccgc ggggctgctg
//
LOCUS       DASVLN010000892         2507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_207924, whole genome shotgun sequence.
ACCESSION   DASVLN010000892 DASVLN010000000
VERSION     DASVLN010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207924"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VF453_20990"
     CDS             complement(<1..1110)
                     /locus_tag="VF453_20990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7690195.1"
                     /translation="MTIVSNAPDKVGKYRITGVIGEGVSGVVYKAFDAAVPRVVAVKS
                     LHGTLAADARAAQAFAARLREQAQAVGRLAHPGVVQVYEIGEYEGRPFIAMEHVAGLN
                     LGQWLGVTPLPAQAVLLEVMEQVLDALEAAHAAGVRHGDVKPGNVLIAGSGQVKLSDF
                     GLARAEGRSGALAGIAPEYVTGRLIDHRVDVYAAGALLYRMLTGREPFADAGDFSGLG
                     ATLRPPSMVAEAQRPQAFDAIVARALAHDPNQRYLSAADFRDALRRIADPRLPERGAR
                     VVTLGGPDDPAAAASFPVLGETPAPSGRQAPVGEAAASVPTLTIAIPDSVLAMPSFDP
                     WAQRGDPLRAANDRRDSELASDSLPGDDAYVAFRDR"
     gene            1469..>2507
                     /locus_tag="VF453_20995"
     CDS             1469..>2507
                     /locus_tag="VF453_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690196.1"
                     /translation="MHSPVAEPAVTHAPARRPGRSLPALVVFSPLRWQFDWQRPQQLL
                     TRLAKHYRVLYVEEPVTTHADDYLAASCPAPGVEVLVPHTRVDAPGFAAAQIAPVQAL
                     LAEHLRRTGVERPLVWLYTPMAQPLVEPLQPRGVLVDVIEDVAADPTAPAVLHEREAA
                     LMALADVVVAGGPSLYQSRRGLHASVHCIPNAVDADHFAPPSPHDTSIEAVSARSLHA
                     VIPGPRLGWFGVVDERLDFALIDRLARLRPDWHLVLAGPVLVDPATLPRRPNIHWIGP
                     QTYAIRPHLLSHWDACILPLRCDEAARHASPPAVLEFLAGQKPVVSTPVHDVVALHGH
                     IVRLAPDADGFA"
BASE COUNT          301 a          967 c          898 g          341 t
ORIGIN      
        1 ccggtcgcgg aaggcgacgt aggcgtcgtc gccgggcagg ctgtcggaag cgagctcgct
       61 gtcgcgccgg tcgttggccg cccgcagcgg atcgccgcgt tgcgcccacg ggtcgaagga
      121 cggcatcgcg agcacgctgt cggggatcgc gatggtcagc gtcggcacgc tggccgcggc
      181 ctcgccgacg ggtgcctggc ggcccgaggg cgccggtgtc tcgcccagca ccgggaagga
      241 cgcggcggct gccgggtcgt cggggccacc cagcgtcacc acgcgcgcgc cgcgctccgg
      301 aaggcgcggg tcggcgatgc ggcgcaaggc gtcgcggaag tcggcggcgc tcaagtagcg
      361 ctggttcggg tcgtgggcga gcgcgcgcgc gacgatggcg tcgaacgcct gcgggcgctg
      421 cgcctcggcc accatgctgg gcggccggag cgtggcgccg aggccggaga agtcgcccgc
      481 gtccgcgaaa ggctcgcggc cggtcagcat gcggtagagc aaggcgccgg cggcgtagac
      541 gtccacgcgg tggtcgatca ggcggcccgt gacgtactcc ggcgcaatgc ccgcgagcgc
      601 gcccgagcgc ccttccgcgc gcgcgagccc gaaatcggag agcttgacct ggcccgagcc
      661 ggcgatcagc acgttgccgg gcttgacgtc gccgtggcgg acgcccgcgg cgtgcgcggc
      721 ctcgagcgcg tccagcacct gttccatcac ctccagcagg acggcctgcg ccggcagcgg
      781 cgtgacgccc agccactgcc cgaggttgag cccggcgacg tgctccatcg cgatgaaggg
      841 acggccctcg tactcgccga tctcgtaaac ctggacgacg cccggatgcg ccagccgccc
      901 gacggcctgc gcctgctcgc gcagccgcgc ggcgaaggcc tgggcggcgc gcgcgtcggc
      961 ggccagcgtg ccatgcagcg acttcaccgc gaccacgcgc ggcaccgcgg cgtcgaaggc
     1021 cttgtagacc acgccgctca cgccttcgcc gatcacgccg gtgatccggt acttgccaac
     1081 cttgtcgggc gcgttgctca cgatcgtcac ggtgtcctca gggcttccct cggcccgctc
     1141 ggcggccgcg ccgggcgaag gcgcgtcgca tcgtcgctgc gggcgatgtt gtcgtgtcgg
     1201 caagtcgccg gagagacctc caacgtcgcc ctcgcccgtg tatccgggct gcgtccggcg
     1261 cgcggccgcg gagggcggcg gctcgtggcc ggaaaggctg tcgggcgatt ctctcatgag
     1321 ggatgcgtcg cggcacgtga tttgccgaag cttcacatgc cgtgcctgag ccaccgttac
     1381 aacgcgttgg cgtcttccgg cacgcggcga cggccggccc cccgccgcgc cggccgccgc
     1441 gcctgacacc cccgtggaga acccgcccat gcattccccc gtcgccgagc cggccgtcac
     1501 ccacgccccg gcccgccgtc ccggccgctc cctgcccgcc ctggtcgtct tctcgccgct
     1561 gcggtggcag ttcgattggc aacgtccgca acaattgttg acccggcttg caaagcatta
     1621 ccgggtgctc tacgtcgagg aacccgtcac cacgcacgcc gacgactacc tggccgccag
     1681 ctgccccgcg ccgggcgtcg aggtgctggt gccgcacacc cgcgtcgacg cgcccggctt
     1741 cgccgccgcc cagatcgcgc ccgtccaggc gctgctcgcc gagcacctgc gccgcaccgg
     1801 tgtcgagcgg ccgctggtct ggctctacac gccgatggcg cagccgctgg tcgagccgct
     1861 gcagccgcgc ggcgtgctcg tcgacgtgat cgaggatgtc gccgcggacc cgacggcgcc
     1921 agccgtgctc cacgaacgcg aggccgcgct gatggcgctg gccgacgtcg tcgtcgcggg
     1981 cgggccctcg ctgtaccagt cgcgccgcgg cctgcacgcg agcgtgcact gcatcccgaa
     2041 cgccgtcgac gccgaccact tcgcgccgcc gtcgccccac gacacgtcga tcgaggcggt
     2101 atcggcgcgc tcgctgcatg cggtgatccc cggcccgcgg ctgggctggt tcggcgtcgt
     2161 cgacgagcgg ctcgacttcg cgctgatcga ccgccttgcg cggctgcggc ccgactggca
     2221 cctggtgctc gcggggcccg tgctggtcga ccccgcgacg ctgccgcgcc gccccaacat
     2281 ccactggatc ggcccgcaga cctacgcgat ccgcccgcac ctgctctcgc attgggacgc
     2341 gtgcatcctc ccgctgcgct gcgacgaggc cgcccgccac gccagcccgc ccgcggtgct
     2401 ggaattcctg gccggccaga agccggtcgt cagcacgccg gtgcacgacg tcgtcgcgct
     2461 gcacggccac atcgtgcggc tggcgcccga cgccgacggc ttcgcgg
//
LOCUS       DASVLN010000893        13220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_477710, whole genome shotgun sequence.
ACCESSION   DASVLN010000893 DASVLN010000000
VERSION     DASVLN010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13220
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477710"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VF453_21000"
     CDS             complement(<1..747)
                     /locus_tag="VF453_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEX7690197.1"
                     /translation="MRSLVASAALVAPLLAAQAAPQSAHEPILPPAGERGLFGTVADK
                     VASGGTELLSQGGQIVTHVRDGATNLVDSAMNFLGVRYKLGGTSAETGFDCSGFTRYV
                     FENSIGHILPHRANEQANDPNLAKVDRKDLKPGDLVFFDTMRRTFSHVGIYLGDGKFI
                     HAPRTGESVRIEDINISYWARRFTGARRVPELANADGANSTDGLLSRLRERLSGRAVV
                     DATAAAGSGPAPLSALSPAVVAALATSTPTL"
     gene            1099..3150
                     /locus_tag="VF453_21005"
     CDS             1099..3150
                     /locus_tag="VF453_21005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7690198.1"
                     /translation="MKPVFLAAAVAMAALLAAAPPADAARKRVAHPAQKRRVPAKAAP
                     AAVPPGPSFPNFQNAPSVASACTVGLEGAQQRAKALEKRRPGLDWLKAWDELYTWEED
                     QSNALTFLKNVHPNADIRAAAETCELRWQDFQSTLSLNEAIYRGGRQSQPLLKDPIDR
                     RAVEVALQGFEDSGVALPADRRAHAKDLSDRIVALGQQFQKNIRDDHTHVAFTEAELK
                     GVPDGVWKDAPRDADGRIELGIDDPSFYPVMQYAEDAGARERMWRAKTNQGGPENLKV
                     LAQITKARLDYAKLLGFPSYADYLLRRRMAENPARVEKFLDDVHAAVADEEKRDIAEL
                     RDAKARQLGQPVDAVKVQRWDPMFYSERLRREKYAVDDEAFRPYFPPQESLRFVMRIA
                     EKLFNVRYDRIEGTWWAPDVQAYSVTDLATNRKIASLWVDLYPRDGKYGGSSVWTLRT
                     PATSVNRPGQAVLVANFDRRGLTLQELEILLHEFGHSLHVDLSATRWASDGGLNVMED
                     FLEAPSQMLESWVYDKKVLKAFREVCPACKPVPDELVDKARAAKDFGKGLKFARQHLF
                     AAYDLALHGPTAVDPMETWQKMEGATPLGYVPGTTFPAGFAHIAGRYGAGYYSYLWSL
                     VVAMDMRTAFAADKLDPKVGARYRADVLSQGGQKPPLQLVHDFLGRDMSTQAFYDYLK
                     K"
     gene            complement(3170..3970)
                     /locus_tag="VF453_21010"
     CDS             complement(3170..3970)
                     /locus_tag="VF453_21010"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX7690199.1"
                     /translation="MPASAPDTLDRPGHVLAFQDEFDRPFLDEARWLPAHLPQWSSRE
                     RARARLDFDEGHLALRIDADQPPWCPEFDGGVRVSSLQTGVSSGPLGSTRGQHRFNRA
                     AVVREAQPRRVLYAPERGLVEMRARACPVPGCHSALWMIGLEDRPEDSTEICVSELFG
                     HDRGTDESVVRFGVHPFGDPRIADDFRHARLPIDTADFHVYALEWTAAGLRFLVDGAE
                     IGSISQSPAYPMQLMLGLYDRTPPGDDAHERLERRFVVDYVRGWRAAG"
     gene            complement(4093..5796)
                     /locus_tag="VF453_21015"
     CDS             complement(4093..5796)
                     /locus_tag="VF453_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7690200.1"
                     /translation="MPLPKPPATRAALLSAADYESQSAPKTGRRSQKAAEPKQKLLDL
                     HDDRAETAVAKPGAKAAGATVTALPVVAKAPAPSPAVVSRAPAPSAPKTTAAPTRRRT
                     RTDGPAKLFVLDTNVLLHDPTSLFRFEEHDVYLPMITLEELDNHKKGMTEVARNARQV
                     SRDLDALATEGSLSPSAGIPLAKTGHKDARGSLFFQTQFLDAKAPSGLPQGKADNQIL
                     AVVQALREQHAPREVVLVSKDINMRVKARALGLPAEDYFNDKTLDDADLLYTGVLALP
                     PDFWDKHGKTMESWNQGGHTFYRIVGPLVPSLLINQFVYLETPGAAPLYARVTEITGK
                     SAVLKTLRDFTHAKNAVWGITARNREQNFALNLLMDPEVDFLTLTGTAGTGKTLATLA
                     CGLAQVMDERRYSEIIVTRVTVPVGEDIGFLPGTEEEKMGPWMGALDDNLEVLAKGDG
                     GAGEWGRAATQDLVRSKIKIKSLNFMRGRTFLNKFVIIDEAQNLTPKQMKTLVTRAGP
                     GTKIVCLGNLAQIDTPYLTEGSSGLTYAVDRFKGWPHSGHITLARGERSRLADFASDA
                     L"
     gene            complement(6014..8341)
                     /locus_tag="VF453_21020"
     CDS             complement(6014..8341)
                     /locus_tag="VF453_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase inhibitor family I36 protein"
                     /protein_id="HEX7690201.1"
                     /translation="MTNLGSHNAAALGLAALASLAAAPAFAQSASDYTQGVAVSGSTA
                     TIWFKSNVNTTWVDVHYQVNGGTQQNVRMTFVNADGRDEQALTVASGNTISYSFTYNN
                     GTPAYDTPVQTYTVGSSTTPGGPVCFFADANYGGDSFCASADSSWVGSTWNDRVSSVK
                     VQAGYEADLYQDINYGGGVVKLTSDTPNLATLNFNDAMSSYHVTTASAIPTSDTPDFG
                     PNVQIFDPSTPATTIQNAIDAAFNAQYQSPTAQFGSQRHAFLFKPGTYSPNARLGFYT
                     TMEGLGLNPDDVRINGNIVVDSGWNYGDTANATQNFWRSAENLSIAPTGGTMEWAVSQ
                     AAPLRHVHVAGNLHMGPTNQGNGQGYSSGGFIADTRVDGIVSTGSQQQWYTRDSNVGQ
                     WYDGVWNTVFSGVGGAPAQSFPSPPYTTLATTPVSREKPYLYVDSSGKYRVFVPNLRT
                     NASGATWSSGSATPGTSIPMSQFYVAKPGDTAATINAALAAGLNLFFTPGVYPLDAAL
                     SVTRANTVVLGLGFPTLQPTAGNNVMNVADVDGVKIAGLLFDAGTTNSATLLQVGAGT
                     SSTSHAANPTSVQDVFFRIGGAVAGKATTSLAVNSNDVLIDDIWAWRADHGSAATGWT
                     INTANTGLLVNGARVLATGLFVEHYQQYQVQWNGENGRTIFFQNEMPYDVPSQAAWTS
                     GNGNGWAAYKVGANVQNHEAWGLGSYCFFNIGGTNTNINAARGFEVPDTAGVKLHSIL
                     TVSLGGNGTISHVVNNTGATAQGTATVPVDLVSYP"
     gene            complement(8650..10056)
                     /locus_tag="VF453_21025"
     CDS             complement(8650..10056)
                     /locus_tag="VF453_21025"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HEX7690202.1"
                     /translation="MPMFPAPTYQARRAALAAHLRGAGARGLVLLPGNVDSPMNYGDN
                     AYDFRQDSNFLYFCGLAQPALALVIDLDEDRATLHGDDIGVDHLVWTGPLPSVAERAA
                     QSGLATTASLADLAATLDGARSAGRDVHFTRPYRGETRLQLAAWLDLPDAALDAAASR
                     PLTLAAVALRAVKSTAEVAEIENALHVTRDMHLLAMRMSRAGIVEQEVVGAMEGLALR
                     HGRRLAYPSIFTSRGEILHNHDHSVRLGGGELVVNDTGANSPIGYASDITRTIPVGGR
                     FRGLQAELYDLALDAQAKAIAAVAPGVPYRDIHELACRTMVAGMTALGFMRGDPGEAV
                     AAGAHAIFFQCGTGHMMGLDVHDMESLGEDAVGYGGGYERSTLFGHKSLRLARPLRPG
                     FVVTIEPGIYINPWLTERWKAEGRHAGFIDYAAFERHAGFGGIRIEDDVLVTADGRRE
                     LGPRIPRTRAEVEAACAD"
     gene            10308..12296
                     /locus_tag="VF453_21030"
     CDS             10308..12296
                     /locus_tag="VF453_21030"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEX7690203.1"
                     /translation="MAIEHLTEEQIKTWTRAQKDEWWLRNVYRGDMPQLTLRSGVMGF
                     LLGGILAATAMYILAKSGIGIGVGLTSVLLSFAFFRVVSNMGIAKDFTILENNCTQSI
                     ATSAGYVIAPLGAGLAAYMVMTGHVLPWWQMVVWLIVVSVTGVLIAFPMKRRFINEDQ
                     LPFPEGRACGVVLDSLYTGGAEVGMFKAKLLAVSAGITALWELLVLDGWERLIQFKIL
                     RMDHWAGLKEPWTLHERLDDYYYMWLTRISEAAPRIMGTDIRALGLRFSLDFSMLGVG
                     GLMGVPVASSVLLGGIVNLAILAPIMIQHGDIVQRVLANGHVVPISRAEIVNQWSIWW
                     GVSMMVVGSCVGLLAKPEIFTSAIKNLFNRKPGEAATRSDVLEDIEVPLWVSWVGVPI
                     FSLLGAWATHDFFGVPWLLSLISLPLIFVLTVICTNSMALTSWMPLGALAKITQFSIG
                     VIDRSNPASNLLPATMTSQISMNAASLLSDIKPGYMLGAKPRHQVWGHVIGLVSGALF
                     SVPLFFLLFLRPDAAGHVSADTIITDNFPMPAAVQWRGVAEIIARGFHGAPTTAIVAA
                     VVAAGAALVLELLRIGTKGRFPLSAVSIGLGVVLPFESCVAMWLGAMLFWVMGRLYPT
                     PGTKANAIWVDGAEPICAGLISAAALMGIVDALLNVFL"
     gene            complement(12324..13121)
                     /locus_tag="VF453_21035"
     CDS             complement(12324..13121)
                     /locus_tag="VF453_21035"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7690204.1"
                     /translation="MPATPLPVDPATALLPGAAGARPPLALLERLFDALPDVVFFAKD
                     AQARYTHGNRTLLERLRLTSRAQLIGRRADELFGRALGDRFLEQDERVLCGGGDVIDQ
                     LELHLYPNRAPGWCLTHKLAWREDGPARGRRAVIGLVGLSRDLAPAGAAHATPPATYA
                     RVAQVVERLQHDYARPLRIAELARGAGLSLAQLERVVAQLYRLTPRQVLARARLDAAI
                     ALLAGDDSIAEIAHACGYADHSAFTRQFRQSTGLTPRAWRTLPQGTR"
BASE COUNT         1916 a         4461 c         4782 g         2061 t
ORIGIN      
        1 cagggtcggc gtgctcgtgg cgagcgcggc gacgacggcc ggcgacaggg cgctcagcgg
       61 cgccgggccg ctgccggccg cggcggtggc gtcgacgact gcccggcccg acagccgctc
      121 gcgcaggcgc gaaagcagtc cgtcggtcga gttggcgccg tcagcgttcg cgagctcggg
      181 cacgcggcgc gcgcccgtga agcgacgcgc ccagtaggag atgttgatgt cctcgatgcg
      241 caccgactcg ccggtgcgcg gcgcgtggat gaacttgccg tcgcccaggt agatgccgac
      301 gtgcgagaag gtgcggcgca tcgtgtcgaa gaacacgagg tcgcccggct tcaggtcctt
      361 gcggtcgacc ttggcgaggt tgggatcgtt ggcctgctcg ttcgcgcgat gcgggaggat
      421 gtggccgatg ctgttctcga agacgtagcg cgtgaagccc gagcagtcga agcccgtctc
      481 ggccgaggtg ccgccgagct tgtagcgcac gcccaggaag ttcatggccg agtcgaccag
      541 gttggtcgcc ccgtcgcgca cgtgcgtgac gatctggccg ccttgcgaga ggagctccgt
      601 gccgccgctg gcgaccttgt ccgcgacggt gccgaacagc ccgcgctcgc ccgcgggcgg
      661 caggatgggc tcgtgggccg actgcggagc cgcctgcgcc gcgagcaggg gcgcgacgag
      721 cgccgccgac gcgaccagac tcctcactcc tgctcgacag gagaggtggc gcgtcgaccc
      781 ggcggccgcg gcccctcggc ttttctcggt aattctcaca gggcaagcat agcggatgcc
      841 agcttttgca gtcaatctgg gggacgggga aaacccgcgc aaatacaaag acttgcgcta
      901 cgaaacgggc tgtttcgaca tggccgcgca cgcattcgag accccgaata tgcaaccggg
      961 cgcaaggcgg tcggaaattc cgacggcacg caacgacaat cgcgcgacgt ccgccgcccg
     1021 accgcaagcg cgccgctgcc cgatccccgg gggcgcgcag ggggcggcag ccccgaatcc
     1081 ccagcgtcat tcccttcgat gaaacctgtc ttcctcgccg ccgccgtcgc gatggccgcg
     1141 ttgctggccg ccgcgccgcc ggcggatgcg gcgcgcaagc gtgtcgccca tcctgcgcag
     1201 aaaaggcgcg tgccggccaa ggccgcgccg gccgccgtgc ccccgggccc gagcttcccc
     1261 aacttccaga acgcgccgtc cgtcgcctcg gcctgcacgg tcgggctgga aggcgcccag
     1321 cagcgcgcca aggcgctcga gaagcgccgc ccgggcctcg actggctgaa ggcctgggac
     1381 gagctctaca cctgggagga ggaccagagc aacgcgctca cgttcctgaa gaacgtccat
     1441 cccaacgcgg acatccgcgc cgccgccgag acctgcgagc tgcgctggca ggacttccag
     1501 tccacgctca gcctgaacga ggcgatctac cgcggtggca ggcagtccca gccgctgctc
     1561 aaggatccga tcgatcgccg cgccgtcgag gtcgcgctgc agggcttcga ggactcgggc
     1621 gtcgcgctgc ccgccgacag gcgcgcgcac gccaaggacc tttccgatcg catcgtcgcg
     1681 ctcggccagc agttccagaa gaacatccgc gacgaccaca cgcacgtcgc gttcaccgag
     1741 gccgagctga agggcgtgcc cgacggcgtc tggaaggacg ccccgcgcga cgccgacggc
     1801 cgcatcgagc tgggcatcga cgacccgagc ttctaccccg tgatgcagta cgccgaggac
     1861 gccggcgcgc gcgagcgcat gtggcgcgcg aagacgaacc agggcggccc cgagaacctg
     1921 aaggtgctcg cgcagatcac gaaggcgcgg ctcgactacg cgaagctgct cggcttcccg
     1981 agctacgccg attacctgct gcgccggcgc atggccgaga acccggcacg cgtcgagaaa
     2041 ttcctcgacg acgtccatgc cgccgtcgcc gacgaggaaa agcgcgacat cgccgagttg
     2101 cgcgacgcga aggcgcgcca gctgggccag cccgtcgacg cggtcaaggt gcagcgctgg
     2161 gatccgatgt tctattccga gcggctgcgg cgcgagaagt acgcggtcga cgacgaggct
     2221 ttccggccgt acttcccgcc gcaggagagc ctgcgcttcg tcatgcgcat cgcggagaag
     2281 ctgttcaacg tccgctacga ccgcatcgaa ggcacgtggt gggcgccgga cgtgcaggcc
     2341 tattcggtca ccgacctcgc gaccaacagg aagatcgcga gcctgtgggt cgacctctac
     2401 ccccgcgacg gcaagtacgg cggctcgtcg gtgtggacgc tgcgcacgcc ggccaccagc
     2461 gtgaaccggc cggggcaggc ggtgctcgtc gccaacttcg accgtcgcgg cctgacgttg
     2521 caggagctcg agatcctgct gcacgagttc ggccactcgc tgcacgtcga tctctcggcc
     2581 acgcgctggg cgagcgacgg cggcctcaac gtgatggagg atttcctcga ggcgccgtcg
     2641 cagatgctgg agagctgggt ctacgacaag aaggtgctca aggccttccg cgaggtgtgc
     2701 cccgcctgca agccggtgcc cgacgagctc gtcgacaagg cgcgcgcggc caaggacttc
     2761 ggcaagggcc tgaagttcgc gcgccagcac ctgttcgcgg cctacgacct cgcgctgcac
     2821 ggcccgaccg ccgtcgaccc gatggagacc tggcagaaga tggaaggcgc cacgccgctc
     2881 ggctatgtcc ccggcacgac cttcccggcc ggcttcgcgc acatcgccgg ccggtacggg
     2941 gccgggtact acagctacct gtggagcctg gtcgtcgcga tggacatgcg caccgccttc
     3001 gcggccgaca agctcgaccc caaggtcggt gcgcgctacc gcgccgacgt gctctcgcaa
     3061 ggcggccaga agccgccgct gcagctggtg cacgacttcc tcgggcgaga catgagcacg
     3121 caggcgttct acgactacct gaagaagtga ggcgagcgcc ggcggcgggt cagcccgccg
     3181 cccgccagcc gcgcacgtag tcgacgacga agcggcgctc gaggcgctcg tgcgcgtcgt
     3241 cgcccggcgg cgtgcggtcg tacaggccca gcatcagctg catcgggtag gccggcgact
     3301 gcgagatgct gccgatctcg gccccgtcga cgaggaagcg caggccggcc gcggtccatt
     3361 cgagggcgta gacgtggaag tccgcggtgt cgatcggcag ccgcgcatgg cggaagtcgt
     3421 ccgcgatgcg cgggtcgccg aacggatgca cgccgaagcg caccaccgat tcgtccgtgc
     3481 cgcgatcgtg gccgaacagc tcgctgacgc agatctcggt ggagtcctcg gggcggtcct
     3541 ccaggccgat catccacaac gccgaatggc agcccggcac cggacatgcg cgggcgcgca
     3601 tctcgacgag gccccgctcg ggcgcgtaga gcacgcgacg cggttgcgcc tcgcgcacga
     3661 cggccgcccg gttgaaccgg tgctggccgc gcgtgctgcc gagcgggccg ctggagacgc
     3721 ccgtctgcag cgaggacacg cgcacgccgc cgtcgaactc ggggcaccac ggcggctggt
     3781 cggcgtcgat gcgcagcgcg agatgaccct cgtcgaagtc gagtcgcgcg cgggcgcgct
     3841 cgcgcgagct ccattgcggc aggtgcgcag gcagccaacg cgcctcgtcg aggaagggac
     3901 ggtcgaattc gtcctggaag gcgaggacgt ggccggggcg gtcgagggta tccggtgcgg
     3961 acgcgggcat ggcgagacgc gcagaaagcg ggccgccatg gtgcctcgtc tcgggcgggc
     4021 ccgaatttca atcgtgtctg cgtcgccggg agcgccgggc tgcctctcgt gtggccttcc
     4081 cggcgcgctg cgtcacaacg cgtccgacgc gaaatccgcc agccgcgacc gctcgccgcg
     4141 cgccagcgtg atgtgcccgc tgtgcggcca gcccttgaag cgatcgaccg cgtaggtcag
     4201 gcccgagctg ccctccgtga ggtagggcgt gtcgatctgc gcgaggttgc ccaggcagac
     4261 gatcttggtg cccggcccgg cgcgcgtgac cagcgtcttc atctgcttgg gcgtgaggtt
     4321 ctgcgcctcg tcgatgatga cgaacttgtt gaggaaggtg cgcccgcgca tgaagttcag
     4381 gctcttgatc ttgatcttgg agcgcaccag gtcctgcgtc gccgcgcggc cccattcgcc
     4441 cgcgccgccg tcgcccttgg cgagcacctc gaggttgtcg tcgagggcgc ccatccacgg
     4501 gcccatcttc tcctcctcgg tgccgggcag gaagccgatg tcctcgccga cgggcacggt
     4561 cacgcgggtg acgatgatct cgctgtagcg ccgctcgtcc atcacctgcg cgaggccgca
     4621 ggccagcgtc gccagcgtct tgccggtgcc cgcggtgccg gtgagcgtga ggaagtcgac
     4681 ctccggatcc atcagcaggt tgagcgcgaa gttctgctcg cggttgcggg cggtgatgcc
     4741 ccagacggca ttcttcgcgt gcgtgaaatc ccgcagcgtc ttgagcaccg cggacttgcc
     4801 ggtgatctcc gtcacgcgcg cgtacagcgg cgccgcgccc ggcgtctcga ggtagacgaa
     4861 ctggttgatc agcagcgacg gcaccagcgg cccgacgatg cggtagaacg tgtggccgcc
     4921 ctggttccag ctctccatcg tcttgccgtg cttgtcccag aaatcgggcg gcagcgcgag
     4981 cacgccggtg tagagcaggt cggcatcgtc gagggtcttg tcgttgaagt agtcctcggc
     5041 gggcaggccc agcgcgcgcg ccttcacgcg catgttgatg tccttcgaca ccagcaccac
     5101 ctcgcgcggc gcgtgctgtt cgcgcagcgc ctgcacgacg gcgaggatct ggttgtcggc
     5161 cttgccctgc ggcaggcccg agggcgcctt ggcgtcgagg aactgggtct ggaagaacag
     5221 gctgccgcgc gcgtccttgt ggccggtctt cgcgagcgga atgccggccg acggcgacag
     5281 cgagccctcg gtggcgagcg cgtcgaggtc gcgcgacacc tgtcgcgcgt tgcgcgcgac
     5341 ctcggtcatg cccttcttgt ggttgtcgag ctcctcgagc gtgatcatcg gcaggtagac
     5401 gtcgtgctcc tcgaaccgga acagcgaggt cgggtcgtgc agcagcacgt tggtgtcgag
     5461 cacgaagagc ttggcggggc cgtcggtgcg cgtgcgccgg cgggtgggcg cggcggtggt
     5521 cttcggcgcg ctcggtgcgg gcgcgcggct gacgacggcg ggcgagggcg caggcgcctt
     5581 ggcgacgaca ggcagcgcgg tgacggtcgc gccggccgcc ttggcgcccg gcttggcgac
     5641 ggccgtctcg gcgcggtcgt cgtgcaggtc gagcagcttc tgcttgggtt ccgcggcctt
     5701 ctggctgcgg cgccccgtct tcggggcgga ttgcgattcg tagtcagcag ccgacagaag
     5761 agctgcgcgg gtggcgggag gcttcggaag tggcatggat cggcgtacga gtcgcgatga
     5821 aggggagggg ctcaggcaac gcccgcggtg cggccgggca caaaaaaagc cgcgcaggaa
     5881 cctgggcggc ttagtcggga gtgcgcgggt cgaaccacaa gcatgggccc atcatacaca
     5941 ttcgggccga tgcgcaagtg acagcccgcg agggtccgcc gcgaggcccg cgccccgcgg
     6001 cggacgcgcg cgatcagggg tagctgacga ggtcgaccgg caccgtcgcc gtgccctgcg
     6061 cggtcgcgcc ggtgttgttg acgacgtggc tgatggtgcc gttgccgccg agcgagaccg
     6121 tgaggatgct gtgcagcttc acgccggcgg tgtccggcac ctcgaagccg cgcgcggcgt
     6181 tgatgttggt gttggtgccg ccgatgttga agaagcagta gctgcccagg ccccaggcct
     6241 cgtggttctg cacgttcgcg ccgaccttgt acgcggccca gccgttgccg ttgccgctgg
     6301 tccaggcggc ctggctcggg acgtcgtagg gcatctcgtt ctggaagaag atcgtgcgcc
     6361 cgttctcgcc gttccactgc acctggtact gctggtagtg ctcgacgaac aggcccgtgg
     6421 ccagcacgcg cgcgccgttg acgagcaggc ccgtgttggc ggtgttgatc gtccagcccg
     6481 tcgcggcgct gccgtggtcg gcgcgccagg cccagatgtc gtcgatcagc acgtcgttgc
     6541 tgttgacggc caggctcgtc gttgccttgc cggcgaccgc gccgccgatg cggaagaaca
     6601 cgtcctgcac cgaggtcggg ttggcggcat ggctcgtcga cgacgtgccc gcgccgacct
     6661 gcagcagggt ggcgctgttg gtcgtcccgg cgtcgaagag gaggcccgcg atcttcacgc
     6721 cgtcgacgtc ggcgacgttc atcacgttgt tgcccgccgt gggttgcagc gtcgggaagc
     6781 ccagccccag gacgacggtg ttggcgcgcg tgacgctcag cgccgcgtcg agcgggtaga
     6841 cgccgggcgt gaagaacagg ttcaggccgg ccgcgagggc cgcgttgatc gtggcggcgg
     6901 tgtcgcccgg cttggcgacg tagaactggc tcatcgggat cgacgtgccg ggcgtcgcgg
     6961 agccgctcga ccacgtggcg ccggaggcgt tggttcgcag gttcggcacg aagacgcggt
     7021 acttgccgct cgagtcgacg tagagatacg gcttctcgcg cgagacaggc gtcgtcgcga
     7081 gcgtcgtgta cggcggcgag gggaaggact gcgccggggc gccgcccacc cccgagaaca
     7141 cggtgttcca cacgccgtcg taccactggc cgacgttgga gtcgcgcgtg taccactgct
     7201 gctgcgagcc ggtggagacg atgccgtcga cgcgcgtgtc ggcgatgaac ccgccgctcg
     7261 aatacccctg gccgttgccc tggttggtcg ggcccatgtg caggttgccc gcgacgtgga
     7321 cgtgacgcag cggcgcggcc tgcgagaccg cccattccat cgtgccgccg gtgggggcga
     7381 tggacaggtt ctcggccgag cgccagaagt tctgcgtcgc gttggcggtg tcgccgtagt
     7441 tccagccgct gtcgacgacg atgttgccgt tgatgcgcac gtcgtccggg ttcaggccga
     7501 ggccttccat cgtggtgtag aaacccaggc gcgcattggg cgagtaggtg cccggcttga
     7561 agaggaaggc atggcgctgc gagccgaatt gcgccgtcgg gctctggtat tgcgcattga
     7621 acgcggcgtc gatcgcgttc tggatcgtcg tcgccggcgt cgacggatcg aagatctgca
     7681 cgttcgggcc gaagtccggc gtgtcggaag tgggaatcgc cgacgcggtc gtgacgtggt
     7741 acgacgacat cgcgtcgttg aagttcagcg tcgcgaggtt cggcgtgtcg gaggtcagct
     7801 tgacgacgcc gccgccatag ttgatgtcct ggtagaggtc ggcctcgtag ccggcctgca
     7861 ccttcaccga cgagacccgg tcgttccagg tcgagccgac ccagctcgag tcggcgctcg
     7921 cgcagaacga atcgccgccg tagttcgcgt cggcgaagaa gcacaccggg ccgcccgggg
     7981 tcgtcgacga gccgacggtg tacgtctgca ccggcgtgtc gtaggccggc gtgccgttgt
     8041 tgtacgtgaa cgaatagctg atcgtgttgc cggaggccac cgtcagcgcc tgctcgtcgc
     8101 ggccgtcggc gttcacgaag gtcatgcgca cgttctgctg cgtgccgccg ttgacctggt
     8161 agtgcacgtc gacccaggtg gtgttgacgt tggacttgaa ccagatcgtc gcggtgctgc
     8221 ccgagaccgc cacgccctgc gtgtagtccg aggcggattg cgcgaaggcc ggcgcggcgg
     8281 ccagggaggc cagcgcggca agaccgagcg cggcggcgtt gtgggaaccg aggttcgtca
     8341 tgtctcttcc tttaaggtgt ttttaaggag caggcggatt gtgggaagcc gccccaagac
     8401 gatgcgaacg gggttgaaac agttttgtta catcatttcg gattgctgcg gcgcaccagt
     8461 tggtaccggt tccatgcgtg gaagcgattt gcgcaatgga attgaatttt ccatttccaa
     8521 ttacaacaag ttgcaattcg ttaatgcgac gataattcgt agttccatgt actacgaaca
     8581 tcacgccggt gcagcgcagc aatcgcccgt ggcgacgaac aatcctcgtc ggccgcggac
     8641 gccgatgact cagtcggcgc aggccgcctc gacctccgcg cgcgtgcgcg ggatgcgcgg
     8701 cccgagctcg cgccggccgt ccgcggtcac gaggacgtcg tcctcgatgc ggatgccgcc
     8761 gaagccggcg tggcgttcga aggccgcgta gtcgatgaag ccggcgtggc ggccctcggc
     8821 cttccagcgc tccgtcagcc aggggttgat gtagatgccc ggctcgatgg tcacgacgaa
     8881 gccgggccgc aggggccgcg ccaggcgcag cgacttgtgg ccgaacagcg tgctgcgctc
     8941 ataccccccg ccgtatccga cggcgtcctc cccgaggctt tccatgtcgt gcacgtccag
     9001 gcccatcatg tggccggtgc cgcattggaa gaagatggcg tgcgcacccg cggccacggc
     9061 ctcgccggga tcgccgcgca tgaagccgag cgccgtcatg cccgcgacca tcgtccggca
     9121 ggccagctcg tggatgtcgc ggtagggcac gccgggcgcg accgcggcga tcgccttcgc
     9181 ctgcgcgtcg agcgcgaggt cgtagagctc ggcctgcagt ccccggaagc ggccgccgac
     9241 cgggatggtg cgcgtgatgt ccgaggcgta gccgatcggc gagttggcgc cggtgtcgtt
     9301 gaccaccagc tcgccgccgc ccagccgcac cgagtggtcg tggttgtgca ggatctcgcc
     9361 gcggctcgtg aagatcgacg ggtacgcgag gcgccggccg tgccgcagcg cgaggccttc
     9421 catcgcgccc acgacctcct gctcgacgat cccggcgcga ctcatgcgca tcgccagcag
     9481 gtgcatgtcg cgcgtcacgt gcagcgcgtt ctcgatctcc gcgacctccg ccgtcgactt
     9541 cacggcccgc agcgcgacgg ctgcgagcgt cagcgggcgg ctcgcggcgg cgtcgagcgc
     9601 cgcgtcgggc agatccagcc aggcggccag ctgcaggcgg gtctccccgc ggtaggggcg
     9661 cgtgaagtgg acatcccggc cggcgctgcg cgcgccgtcc agcgtcgcgg cgaggtcggc
     9721 gagcgacgcg gtcgtcgcca ggcccgactg cgcggcgcgc tcggcgacgc tcggcagcgg
     9781 acccgtccag accaggtggt cgacgccgat gtcgtcgccg tgcagcgtcg cgcggtcctc
     9841 gtcgaggtcg atcaccagcg cgagcgccgg ctgcgcgagg ccgcagaagt acaggaagtt
     9901 cgagtcctgg cggaagtcgt acgcgttgtc gccgtagttc atcgggctgt cgacgttgcc
     9961 cggcagcagc acgagcccgc gcgcccccgc gccgcgcagg tgcgcggcca gggccgcgcg
    10021 gcgagcctgg tacgtggggg cagggaacat cggcatcggt ggaactccgt ggttggcgag
    10081 cgtttccgag gcgcgtgcct cgaggggcgg gcggccggat tatgggcgcc gcctccgcgg
    10141 ccggcctacg cgttacctcg gacggccggg ccgtccctcg gctgagacaa tgcgccccgc
    10201 acccggcacc gcggcgatcc cgcggccggc gtgcgcccgg gcgcgcgcgg gtcggccacg
    10261 acaggccggc gtcgtcccgg acgacctcga aaccactcga cacgaccatg gcgattgagc
    10321 acctcaccga agaacagatc aagacctgga cgcgcgccca gaaggacgaa tggtggctgc
    10381 gcaacgtcta ccggggcgac atgccccagc tcacgctgcg ctccggcgtg atgggcttcc
    10441 tgcttggcgg catcctagcg gccacggcga tgtacatcct cgccaagtcg ggcatcggca
    10501 tcggcgtggg gctgacctcg gtgctgctgt ccttcgcgtt cttccgcgtc gtgtccaaca
    10561 tgggcatcgc gaaggacttc acgatcctgg agaacaactg cacgcagtcg atcgccacgt
    10621 cggcgggcta cgtgatcgcc ccgctgggcg cgggcctggc cgcctacatg gtgatgacgg
    10681 gccacgtgct gccgtggtgg cagatggtgg tgtggctgat cgtcgtgtcg gtgacgggcg
    10741 tgctgatcgc gttcccgatg aagcggcgct tcatcaacga ggaccagctc ccgttccccg
    10801 agggccgcgc ctgcggcgtc gtgctcgact cgctgtacac cggcggcgcc gaggtcggca
    10861 tgttcaaggc caagctgctc gcggtctcgg ccggcatcac ggcgctctgg gagctgctcg
    10921 tcctggacgg ctgggaaagg ctgatccagt tcaagatcct gcgcatggac cactgggccg
    10981 gcctgaagga gccgtggacg ctccacgagc gcctggacga ctactactac atgtggctca
    11041 cgcgcatcag cgaggccgcg cccaggatca tgggcaccga catccgcgcg ctcggcctgc
    11101 gcttctcgct cgacttctcg atgctcggcg tcggtggcct gatgggcgtg ccggtcgcct
    11161 ccagcgtgct gctgggcggc atcgtcaacc tcgcgatcct cgcgccgatc atgatccagc
    11221 acggcgacat cgtgcagcgc gtgctggcca acggccacgt ggtgccgatc tcgcgcgcgg
    11281 agatcgtcaa ccagtggtcg atctggtggg gcgtgtcgat gatggtggtg gggtcgtgcg
    11341 tgggcctgct ggccaagccc gagatcttca cgagcgcgat caagaacctc ttcaaccgca
    11401 agcccggcga ggccgcgacg cgcagcgacg tgctcgagga catcgaggtg ccgctgtggg
    11461 tatcgtgggt cggcgtgccg atcttcagcc tgctgggcgc gtgggcgacg cacgacttct
    11521 tcggcgtgcc gtggctcctg tcgctgatct cgctgccgct gatcttcgtg ctgaccgtca
    11581 tctgcaccaa ctcgatggca ctgacctcgt ggatgccgct gggcgcgctc gccaagatca
    11641 cgcagttcag catcggcgtc atcgaccgca gcaatccggc gagcaacctg ctgccggcga
    11701 cgatgacctc gcagatctcg atgaacgccg ccagcctgct gtcggacatc aagcccggct
    11761 acatgctggg cgccaagccg cggcaccagg tctggggcca cgtgatcggc ctcgtgtcgg
    11821 gcgcgctgtt ctccgtgccg ctgttcttcc tgctgttcct gcgcccggac gccgcgggcc
    11881 acgtcagcgc cgacacgatc atcaccgaca acttcccgat gcccgcggcc gtgcagtggc
    11941 gcggggtggc cgagatcatc gcgcgcggct tccacggcgc gccgacgacg gcgatcgtcg
    12001 cggccgtggt cgcggccggc gcggcgctgg tgctggagct cctgcgcatc ggcaccaagg
    12061 gtcgcttccc gctgtcggcg gtgtcgatcg gcctgggcgt ggtgctgcct ttcgagtcct
    12121 gcgtcgcgat gtggctgggc gcgatgctgt tctgggtcat ggggcgcctg tacccgacgc
    12181 ccggcaccaa ggccaacgcg atctgggtcg acggcgccga gccgatctgc gccggcctga
    12241 tctcggccgc ggcgctgatg ggcatcgtcg acgcgctgct gaacgtgttc ctctgacgcg
    12301 tcgcctcggg gcgcgtcggt cgatcagcgc gtcccctgcg gcagcgtgcg ccaggcgcgc
    12361 ggcgtgagtc cggtgctctg gcggaactgg cgcgtgaacg cgctgtggtc ggcatagccg
    12421 caggcatgcg cgatctcggc gatcgaatcg tcgcccgcca gcagcgcgat ggccgcgtcc
    12481 agccgcgcgc gcgcgagcac ctgccgcggc gtcagccggt acagctgcgc gacgacccgc
    12541 tcgagctgcg ccagcgacag cccggcgccg cgtgccagct ccgcgatgcg cagcggccgg
    12601 gcgtagtcgt gctggaggcg ctcgaccacc tgcgcgacgc gcgcatacgt ggcgggcggc
    12661 gtcgcgtgag ccgcgcccgc aggcgcgagg tctcgcgaca gcccgaccag cccgatcacc
    12721 gcgcgccgcc cgcgcgccgg cccatcctcg cgccacgcga gcttgtgcgt gaggcaccag
    12781 cccggcgcgc ggttggggta caggtgcagc tcgagctggt cgatgacgtc gcccccgccg
    12841 cacaacaccc gctcgtcctg ctcgaggaag cggtcgccca gcgcgcggcc gaacagctcg
    12901 tccgcgcggc ggccgatcaa ttgtgcgcgg ctcgtcaggc gcaggcgttc cagcagcgtg
    12961 cggttgccgt gggtgtagcg ggcctgggca tccttggcga agaagaccac gtcgggcagg
    13021 gcgtcgaaaa gccgctccag caaggccagc ggcggacgcg cgccggcggc gccggggagc
    13081 agggcagtgg cgggatcgac cggcaacggc gtggcgggca tggacggaat tgtgccgaaa
    13141 tcgtcacgcc gtcggcggca agcgatcaag ccggcgaccg gcggggcgcc cagaatcggc
    13201 gccatgcagc gcatcaccgt
//
LOCUS       DASVLN010000894         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_44960, whole genome shotgun sequence.
ACCESSION   DASVLN010000894 DASVLN010000000
VERSION     DASVLN010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44960"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VF453_21040"
     CDS             <1..256
                     /locus_tag="VF453_21040"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7690205.1"
                     /translation="RADTTAGIGFWVQLGAFGKRDGALDFQRKLIDEQPWLAPLLAVF
                     ADRGLNKLQAGPYPTREEARGVAARVRAALHLVPTIVEKR"
     gene            complement(427..714)
                     /locus_tag="VF453_21045"
     CDS             complement(427..714)
                     /locus_tag="VF453_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690206.1"
                     /translation="MSASLTSGTYGRQPALSGSVRQFLSSVVKAFGKADGVSYAAAAA
                     TGRQERAATSFRTRVREANDVRELARSVELHSPGFAADLYAAASRHEGLDD"
     gene            853..1797
                     /locus_tag="VF453_21050"
     CDS             853..1797
                     /locus_tag="VF453_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7690207.1"
                     /translation="MNFRTLDLNLLRVFDAVMAEGSLTRAAERLAMTQPAASHALKRL
                     HTAVGEELFQRSATGMRPTVRAEALWPQVRAALAQLQAALSPRSFDPARDAATFNIAM
                     ADATAAMLLPGLVSAVEQAEALANIRVLPLTTRDPRRMVEAGDADLAIGYFPTAVASI
                     VAQGADATLHHAPLYASEYVCVMRRDHPLATQELTLDAFVDARHLLVSFSGRAHGFVD
                     EALAGLGRQRRVMLTVNQFATAGTIVARSDLLTVLPRQFLPATGAEHRLVQKPLPMPV
                     APIQVVMMWHLRKDDDPPHRWLRERVIDAASGRPALRR"
     gene            complement(2111..3301)
                     /locus_tag="VF453_21055"
     CDS             complement(2111..3301)
                     /locus_tag="VF453_21055"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEX7690208.1"
                     /translation="MFQHIEPFAGDPILSLNEDFQKDPRANKINLSIGIYFDDAGRIP
                     VLDCVRQAEAQLLDKSGPKPYLPIEGDPEFRRAVQTLLFGANSPAVAEGRVATLQTIG
                     SSAALKVGADFLKRWFPDSGVWVSDPTWDNHRSMFEGAGIRVSSYPYYDPATGGVKFD
                     AMREALSQLPARSVVLLHACCHNPTGVDLTRAQWDELIPVLKSRELLPFLDIAYQGYG
                     DGIEEDAYAIRALASAGVAFFVANSFSKNLSVYGERAGGLSVVCPSAAEAGLALGQLK
                     ATVRRIYSSPTIHAGGIVARVFGDPALQASWRKDVDAMRSRIQAMRRQLHEVLSAKVP
                     GRDFNYFLTQRGMFSYTGLSAAQVDRLREEFGVYLIRSGRICVAGLNTRNVEATANAI
                     AAVV"
BASE COUNT          507 a         1283 c         1332 g          497 t
ORIGIN      
        1 ccgcgccgac accacggccg gcatcggctt ctgggtccag ctgggcgctt tcggcaagcg
       61 cgacggcgcg ctggacttcc agcgcaagct catcgacgag caaccgtggc tcgcgccgct
      121 gctggcggtc ttcgccgacc ggggcctcaa caagctgcag gccgggccgt acccgacgcg
      181 cgaggaggcc cgcggcgtgg cggcacgcgt gcgcgcagcg ctgcatctcg tgccgacgat
      241 cgtcgagaag cgctgagacg gcaagcgcgc ggccgctgcg cgatgggggc cgctcggggg
      301 cggcacatcc gccgccgggg cttcgaatcg cgccgacgca ttcaggcgag cgccgcgacc
      361 ggcgcccgcg tgttcggccg cccgtccgga cggccaggcc ggatcacgcg acccggccgc
      421 ggacgatcag tcgtccaggc cttcgtggcg cgaggcggcg gcgtacaggt cggcggcgaa
      481 gcccggcgag tgcagctcga cgctgcgcgc gagttcgcgg acgtcgttgg cctctcgcac
      541 gcgggtgcgg aacgaggtgg cggcgcgctc ctggcggccc gtcgcggcgg ccgccgcgta
      601 gctgacgccg tcggccttgc cgaaggcctt gacgacggaa ctcaggaatt ggcggacgga
      661 gcccgacagg gccggttggc ggccataggt accggaggtg agcgaagcgg acatgatgtt
      721 gtcttccctt gcagagcgcc tcgatgaagg cgcgatggaa gaactgtaag ggttaacccc
      781 cgatgatgcc aatgaatgtt ggacatccgg gatattcata atgcgcatgt cagtccgtac
      841 ttccgacccg tcgtgaattt ccgcacgctc gatctcaacc tgctgcgcgt cttcgatgcc
      901 gtgatggccg aaggcagtct gacccgcgcc gccgagcggc tcgcgatgac ccagccggcc
      961 gccagccatg cgctcaagcg gctgcacacg gcggtcggcg aggagctgtt ccagcgcagc
     1021 gcgaccggga tgcgcccgac cgttcgcgcg gaggcgctgt ggccgcaggt gcgcgccgcg
     1081 ctcgcccagc tgcaggcggc gctgtcgccg cgcagcttcg atccggcccg ggacgccgcg
     1141 accttcaaca tcgcgatggc cgacgcgacg gcggcgatgc tgctgccagg gctcgtctcg
     1201 gcggtcgagc aggcagaggc gctcgccaac atccgcgtcc tgccgctgac cacgcgcgac
     1261 ccgcgccgca tggtcgaggc cggcgacgcc gacctggcca tcggctactt cccgaccgcg
     1321 gtcgcctcca tcgtcgccca aggcgccgac gcgacgctgc accacgcgcc gctgtacgcg
     1381 tccgaatacg tctgcgtcat gcggcgcgac catccgctcg cgacgcagga gctcacgctc
     1441 gacgccttcg tggacgcgcg ccacctcctg gtgagttttt ccggccgcgc gcacggcttc
     1501 gtcgacgagg cactcgcggg cctgggccgc cagcgccggg tcatgctgac cgtcaaccag
     1561 ttcgcgaccg caggcacgat cgtcgcgcgc tccgacctgc tgaccgtgct gccgcgccag
     1621 ttcctgcccg cgaccggcgc cgagcaccgg ctggtgcaga agccgctgcc aatgcccgtc
     1681 gcgcccatcc aggtcgtgat gatgtggcac ctgcgcaagg acgacgatcc gccccaccgg
     1741 tggctgcgcg agcgcgtcat cgacgccgcc tccggccgcc cggcgctgcg gcgctgaagc
     1801 gccgacgtgg tcgccaggtc ggcccgatcg cgaagcgcct ttccgggccg gcgctgccgc
     1861 tcggacggcc ccagcgagac acggggaagc gacggatccg gcttcgccgg gccgcgcacg
     1921 ccccctcggg ggcaggcccc tgcggcgccg aggcggacgc caggtcggcc cgatcgcgaa
     1981 gcgcctttcc gggccggcgc tgccgctcgg acggccccag cgggacacgg ggaagcgacg
     2041 gatccggctt cgccgggccg ttcgcgcacg ccccctcggg ggggcaggcc gcgctagcgg
     2101 ccgtgggggc tcacaccact gcggcgatgg cgttcgcggt cgcctcgacg ttgcgcgtgt
     2161 tcagtcccgc gacgcagatg cgcccggaac ggatcaggta gacgccgaac tcctcgcgca
     2221 gacgatcgac ctgcgccgcc gacaggccgg tgtagctgaa catgccgcgc tgcgtcagga
     2281 agtagttgaa gtcgcggccg ggcaccttgg cgctgagcac ctcgtgcagc tgcctgcgca
     2341 tcgcctggat gcgggagcgc atcgcgtcca cgtccttgcg ccaggaggcc tgcagcgccg
     2401 gatcgccgaa gacgcgggcg acgatgccgc cggcgtggat ggtcgggctc gagtagatgc
     2461 ggcgcaccgt cgccttgagc tggccgagcg cgaggcccgc ctcggcggcg ctcgggcaga
     2521 cgacgctcag cccgccggcg cgctcgccgt agacgctcag gttcttggag aacgagttgg
     2581 cgacgaagaa cgccaccccg gccgaggcca gggcgcggat cgcgtaggcg tcttcctcga
     2641 tgccgtcgcc gtagccctgg taggcgatgt cgaggaaggg cagcagctcg cgcgacttca
     2701 ggacggggat cagctcgtcc cactgcgcgc gcgtgaggtc gacgccggtc gggttgtggc
     2761 agcaggcgtg cagcagcacg acgctgcggg caggcagctg cgacagcgcc tcgcgcatcg
     2821 cgtcgaactt cacgccgccg gtggccgggt cgtagtacgg gtagctcgag acgcggatgc
     2881 cggcgccctc gaacatcgaa cggtggttgt cccaggtcgg gtcgctgacc cagacgccgg
     2941 agtcggggaa ccagcgcttg aggaagtccg cgccgacctt cagcgccgcg ctcgagccga
     3001 tcgtctgcag cgtggcgacg cggccctcgg ccaccgccgg cgagttcgcg ccgaacagca
     3061 gcgtctgcac ggcgcgccgg aactcggggt cgccctcgat gggcaggtag ggcttcggcc
     3121 cgctcttgtc gagcagctgc gcctcggcct ggcgcacgca atcgagcact gggatgcggc
     3181 cggcgtcgtc gaagtagatg ccgatggaca ggttgatctt gttcgcgcgc ggatccttct
     3241 ggaagtcctc gttgagggag aggatcggat caccggcgaa cggttcgatg tgctggaaca
     3301 tgggcgggct cgggttgcgg cgttgcgaat cagggcgaac ccggattatg gagcgcccct
     3361 gccccgccgt ggtgcgccaa tccggccatt tcccggggga cttgcgggcc gcgcgcgtcc
     3421 gaagacgaag gaagtgcggt agccgccgtc gtcagccgcc ccagcgcacc cagcggccat
     3481 gcgcatgcga tcgtgcgccc aggcgccagg cgatgccgcc gccgcgagcg gccgtggcga
     3541 cggtccaggc ggtggcgtgc gcgagctccg cgggatcgac cgagccggca ggtgccggcg
     3601 gcgccggctc gggcagcgc
//
LOCUS       DASVLN010000895         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_44988, whole genome shotgun sequence.
ACCESSION   DASVLN010000895 DASVLN010000000
VERSION     DASVLN010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44988"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="VF453_21060"
     CDS             complement(<1..1641)
                     /locus_tag="VF453_21060"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX7690209.1"
                     /translation="MDRVADPAPPGAAVPAERPVRRIGRVRTRLLAAFLLLLGATVLL
                     TAVGWVGLRRMQATLAGVQDDLLPRISQALQLSERAAQLASTAPKLGDALSSAELDAH
                     RQSVEALLAQIGRGSAEIGQTTPQLRGVIDRLQESVHQHLPALIRLSQQKLQAQAQLD
                     GELSRLDRLGQALHAPRAGEPVDPRISSLWSTLVLGAAVSDAATIGRLEADAEALLLS
                     ARRRDALAALPPDEAAELVALTEGDGSVLACRQSLVNLDAQIDYLVTLTRANADDLGE
                     ETSRYVAQLRLAAADRSERMRVAARASEALMLALTALCVAIGAFATRYVRRLVGEIET
                     ITHVMSRLASGDTAQATPATARPDELGALARSFEVFRDNLLAKQQLVRDLHAQRELLE
                     AVHDNLTDGLAVFDRDGRLLLWNNRLVEMLGRQAVTPAAGSTPAALLAAMPAGTTWSS
                     PESQRHEPLERLAPEGWLRAEHLELRAPDGQVLDLRSCAMPGGGTVSLVTDLSARRAI
                     ETQLQQAQKLEVLGQLTGGVAHDFNNYLGTILGNLALLE"
     gene            complement(1647..2414)
                     /locus_tag="VF453_21065"
     CDS             complement(1647..2414)
                     /locus_tag="VF453_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7690210.1"
                     /translation="METTSVDVMETTRPHVLVVDDEPDMVALLQDVLRRAGFEADGAG
                     CGDDARRAMAQRQYALMLLDLRLKAEDGLTLARQMRQASPIPIIMISGSSDETDRVLL
                     LELAADDFLVKPFSSRELIARVRAVLRRYDMAPQPAPAPPGRAPAAGSERLSFGDWTL
                     DLTERELHRADGSLCALTQAEFRLLEAFVRQPRRIWTRDQLLEQTRSLDTEVFDRTID
                     VLILRLRRKIEPNPKHPSYICTERGMGYVFSADVTRC"
     gene            2675..2977
                     /locus_tag="VF453_21070"
     CDS             2675..2977
                     /locus_tag="VF453_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX7690211.1"
                     /translation="MSSPVQVPAAGDPQSLAPLERELAELLVKALNLEVAVSDIDPAA
                     PLYGDGLGLDSIDILEIALEVSQRYGFELRSDDENNVRIFASLRSLAAHVAAHRSR"
BASE COUNT          408 a         1136 c         1156 g          418 t
ORIGIN      
        1 ctcgagcagg gccaggttgc cgaggatggt gccgaggtag ttgttgaagt cgtgcgcgac
       61 gccgccggtc agctggccca gcacctccag cttctgggcc tgctgcagct gggtctcgat
      121 cgcgcgccgg gcgctcaggt cggtcacgag cgagaccgtc ccgcccccgg gcatcgcgca
      181 gctgcgcagg tcgagcacct ggccgtcggg cgcgcgcagc tccaggtgct cggcgcgcag
      241 ccagccctcg ggcgcgaggc gttcgagcgg ctcgtggcgc tgcgattccg ggctgctcca
      301 ggtcgtccct gcgggcatcg cggcaagcag cgccgccggc gtcgagcccg cggccggcgt
      361 cacggcctgg cggccgagca tctcgacgag gcggttgttc cacagcagca gccggccgtc
      421 gcggtcgaag acggccaggc cgtcggtgag gttgtcgtgg acggcctcga gcagctcgcg
      481 ctgcgcatga aggtcgcgca ccagctgctg cttggcgagc aggttgtcgc ggaacacctc
      541 gaagctgcgc gcgagcgcgc cgagctcgtc gggccgcgcc gtcgccggcg tcgcctgcgc
      601 cgtgtcgccg gaggccaggc gcgacatcac gtgggtgatc gtctcgatct cgccgaccag
      661 gcgccgcacg tagcgcgtcg cgaacgcgcc gatggccacg cacagcgcgg tcagcgcgag
      721 catcagcgcc tcgctcgcgc gcgcggccac gcgcatgcgc tcgctgcggt cggccgcggc
      781 cagacgcagc tgggcgacgt agcgcgaggt ctcctcgccg aggtcgtcgg cgttggcgcg
      841 cgtcagcgtg acgaggtagt cgatctgcgc gtccaggttg acgagcgact gccggcacgc
      901 gagcaccgaa ccgtcgccct ccgtcagcgc caccagctcc gcggcctcgt ccggcggcag
      961 cgcggcgagc gcgtcgcgcc ggcgcgcgga cagcagcagc gcctcggcat cggcctcgag
     1021 gcggccgatc gtcgccgcgt ccgacaccgc ggcgcccagc accagcgtcg accacaggct
     1081 ggagattcgc ggatccaccg gctcgcccgc gcgcggcgcg tgcagggcct ggccgaggcg
     1141 gtccagccgc gagagctcgc cgtccagctg cgcctgcgcc tgcagcttct gctgcgacaa
     1201 ccggatcagc gccggcaggt gctggtgcac gctctcctgg aggcgatcga tgacgccgcg
     1261 cagctgcggc gtcgtctggc cgatctcggc ggagccgcgg ccgatctggg cgagcagcgc
     1321 ctccacgctc tggcgatggg cgtcgagctc ggcgctcgag agcgcgtcgc ccagcttcgg
     1381 cgcggtgctg gcaagctggg cggcgcgctc cgacagctgc agcgcctgcg agatccgcgg
     1441 caacaggtcg tcctgcacac ccgcgagcgt cgcctgcatg cgccgcaggc cgacccagcc
     1501 caccgccgtc agcagcaccg tcgccccgag cagcagcagg aaggccgcga gcagccgcgt
     1561 gcggacgcgc ccgatgcgcc gcacgggccg ttcggccggg acggcggcgc cgggcggggc
     1621 ggggtcggcg acgcggtcca cggcggtcag cagcgcgtca cgtcggcgga gaagacgtag
     1681 cccatgccgc gctcggtgca gatgtaggac gggtgcttgg gattgggctc gatcttgcgg
     1741 cgcaggcgca ggatcaggac gtcgatggtg cggtcgaaga cctcggtgtc gaggctgcgg
     1801 gtctgctcga ggagctggtc gcgcgtccag atgcggcggg gctggcgcac gaaagcctcg
     1861 agcagccgga actccgcctg ggtgagcgcg cacaggctgc cgtcggcgcg gtgcagctcg
     1921 cgctcggtga ggtcgagcgt ccagtcgccg aaggacagcc gttcgctgcc ggccgccggc
     1981 gcgcggcccg ggggcgccgg ggccggctgg ggcgccatgt cgtagcggcg cagcacggcg
     2041 cgcacgcggg cgatcagctc gcgggagctg aagggcttga ccaggaagtc gtcggccgcg
     2101 agctccagga gcagcacgcg gtcggtctcg tcgctgctgc cgctgatcat gatgatcggg
     2161 atcggcgagg cctggcgcat ctggcgcgcg agcgtcaggc cgtcctcggc cttgagccgc
     2221 aggtccagca gcatgagcgc gtactggcgc tgcgccatcg cgcgccgcgc gtcgtcgccg
     2281 cagccggcgc cgtcggcctc gaagccggcg cggcgcagga cgtcctgcag cagcgccacc
     2341 atgtcgggct cgtcgtccac cacgagcacg tgggggcgtg tcgtctccat cacgtccacg
     2401 gatgtcgtct ccatcgtgta catggcgacg cgcgcgctgc gccgcccggc tcctcgggcg
     2461 ggagctgtcc aggagtggaa catggcacga agggccccgt gtttcaacta tttcaaaaaa
     2521 gatcccccgt gtgagggacg ccacgggccg cgcccgccgg gaaaatccag gcgtccgtcc
     2581 cgtgcgttcc tgcgcaccgg cacatgggtt gccgccgttg ccgggcttta caatccgccc
     2641 ttcagccaga acacagccaa tcagggaagc acccttgtcc tcgcccgtcc aagtccccgc
     2701 cgccggcgac ccgcaatcgc tcgccccgct cgagcgcgag ctggccgagc tgctcgtcaa
     2761 ggccctcaac ctcgaggtcg ccgtgagcga catcgacccc gccgcgcccc tgtacggcga
     2821 cggcctgggc ctggactcga tcgacatcct cgagatcgcg ctggaggtct cgcagcgcta
     2881 cggcttcgag ctgcgctccg acgacgagaa caacgtccgc atcttcgcgt cgctgcgcag
     2941 cctggcggca cacgtcgccg cccatcgcag ccgctgaacg catgaaggcc acgggcacca
     3001 tcgcctgggc ggtggccggc gcggcggcct acgccgcggc ctcgcacctg ctgatgacgc
     3061 gcgccgccga cgcgccctgg gccgtcgcgg tgctgctcgg gccgctcgtg gccgcggg
//
LOCUS       DASVLN010000896         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_532356, whole genome shotgun sequence.
ACCESSION   DASVLN010000896 DASVLN010000000
VERSION     DASVLN010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532356"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1380
                     /locus_tag="VF453_21075"
     CDS             64..1380
                     /locus_tag="VF453_21075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690212.1"
                     /translation="MNPNDGTALACSGLPAADPCADALPDSPQCALNYHFGMLLGVDD
                     FRAEQGFHVGRLRRHQRLLHGAGVVAGYPITYDTASFDLAVGPGCAIDVLGRDLCLDE
                     RRCVNLAQWWQKHSADDEFAALDPADATVDLDIRLCYATCLDRAVPAIAQPCAGDAAD
                     VAFSRLCETVSLALVKHVDPAPAVSSAQPAHLLRLWLGLDAAAVDAQGHPLPDDQWLG
                     DRVDAIEALPVEQQGPARAALLREVTARAVAAIDPASPELDGDAEACIVLGTLRQVHF
                     TRDADGWHVSVGGTDGVELGGRPMLLTTSVLQALALADPPASPAPAGPIVVVGGARLS
                     GTEVALTFSQPLAAASVTPQAFAIGEFDDTHGWRAFTIAAPTTDTTHPERPVVTLALD
                     RAPTGAILRITVVGDGPAPLLGGRWIPAGAWRADGDGRNLTTTLTL"
     gene            1390..>3147
                     /locus_tag="VF453_21080"
     CDS             1390..>3147
                     /locus_tag="VF453_21080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690213.1"
                     /translation="MGTSTPTPDDVVDLSSADGVAFIAQSTPLTRLHYFDGKFLRADA
                     FTLEQDYHRAAIRLANVAGGWGIVDGLGVALDAKDDGLLDVGAGLAVTAAGSLVYAVG
                     DLQAKLSDLLATATPAPNGGGNADFGDCAAAPKAGITQAAGLGLYEITIGPVDGLCGN
                     EAVFGKICESACASDSQRPYWREGVVLRLRPIALQLPASTAVALTTTHLRNRVASAYF
                     AAEPWLTPSALSAAGLDSDLWCNPARLYGRDEVVIGLLVREGTATRVIDAWSGRRERM
                     DAQARGYWQGRMAMRPWNVFVAQVLQFQCQLSGLFDASSGLTGGGDCERIRRLLDSTR
                     MQIDALHRRYSEGALRILQKFGNKPTAADAQSMVNELKGSYADLYEISQGLAGADVGA
                     GALPVVRMLLNAGFVELPPAGYLPVVPGQQPLAAQLSRMFGEGVALTFHAVRHDEIAH
                     LVEEAQHLDRISLTRGLDDPAQVEQVEVFVPDGTQTDAATQAPGRWWQAALQVDALGD
                     VDLGLERSTYTLAPERTTAGGGQATDVRPVTGMLGLRSARASAPAAQAPAPASAKKRT
                     AVGAQQVAAPPAQSIGTARP"
BASE COUNT          426 a         1207 c         1115 g          399 t
ORIGIN      
        1 ggccgcgacg cgcccgcgcc tcgggccgca gcgccgagcg gccctgacgc aagggagatg
       61 gccatgaacc cgaacgacgg caccgcgctc gcctgcagcg gcctgcccgc ggccgacccg
      121 tgcgcggacg cgctgcccga cagcccgcaa tgcgcgctga actaccactt cggcatgctg
      181 ctgggtgtcg acgacttccg cgcagagcag ggcttccacg tcggccgcct gcggcgccac
      241 cagcgcctgc tgcacggcgc cggcgtggtc gctggatacc ccatcacgta cgacacggcg
      301 agcttcgacc tcgccgtcgg cccgggctgc gccatcgacg tcctcggtcg tgacctctgc
      361 ctcgacgagc gccggtgcgt gaacctcgcg caatggtggc agaagcactc ggccgacgac
      421 gagttcgcgg cgctcgaccc ggcggacgcg accgtcgatc tcgacatccg cctgtgctac
      481 gcgacctgcc tggaccgcgc cgtgcccgcc atcgcgcagc cgtgcgccgg cgacgcggcc
      541 gacgtcgcct tctcgcgcct gtgcgagacc gtctcgctcg cgctcgtcaa gcacgtcgac
      601 cctgcgcccg cggtgtcctc ggcgcagccg gcgcacctgc tgcggctgtg gctcgggctc
      661 gatgcggcgg cggtcgacgc ccagggccac ccgctgcccg acgaccagtg gctcggcgac
      721 cgcgtcgacg cgatcgaggc gctgcccgtc gagcagcagg ggcctgcgcg cgcggcgctg
      781 ctgcgggagg tgacggcgcg tgcggtcgcg gccatcgatc ccgcctcgcc ggagctcgat
      841 ggcgacgccg aggcctgcat cgtgctgggc accctgcggc aggtgcactt cacgcgggac
      901 gccgacggct ggcacgtaag cgtgggcggc acggacggcg tcgagctggg cggccggccg
      961 atgctgttga ccacgagcgt gctgcaggcg ctcgcgctcg ccgacccacc ggcctcgcct
     1021 gcgcctgccg ggccgatcgt cgtggtcggc ggtgcgcgcc tctcgggcac cgaggtcgcg
     1081 ctgaccttca gccagccgtt ggccgccgcg agcgtcacgc cgcaagcctt cgcgatcggc
     1141 gagttcgacg acacgcacgg ctggcgcgcg ttcacgatcg cagcgcccac caccgacacg
     1201 acccaccccg agcgtcccgt cgtcaccctg gcgctcgatc gtgcgcccac cggcgcgatc
     1261 ctgcgcatca ccgtcgtcgg cgacggcccc gcgccgctct tgggcggccg gtggatcccg
     1321 gccggcgcgt ggcgcgcgga cggcgacggt cgcaacctca ccaccaccct caccctgtag
     1381 aggccaccca tgggcacgag caccccgacc ccggacgacg tcgtcgacct gagcagcgcc
     1441 gacggcgtcg ccttcatcgc gcagtcgacg cccctgacgc ggctgcacta cttcgacggc
     1501 aagttcctgc gcgccgacgc gttcacgctg gagcaggact atcaccgcgc cgcgatccgc
     1561 ctcgccaacg tcgcgggcgg ctggggcatc gtcgacggcc tgggcgtcgc gctcgacgcc
     1621 aaggacgacg gcctgctcga cgtcggcgcc ggcctcgcgg tgacggcggc cggcagcctc
     1681 gtctacgcgg tgggcgacct ccaggcgaag ctgtccgacc tgctggcgac ggccacgccc
     1741 gcccccaacg gcggcgggaa cgccgacttc ggggactgcg cggccgcacc caaggccggc
     1801 atcacgcagg cggcggggct gggcctctac gagatcacga tcggcccggt cgacggcctg
     1861 tgcggcaacg aggccgtctt cggcaagatc tgcgaaagcg cgtgcgcgag cgactcgcag
     1921 cgcccctact ggcgcgaggg cgtggtgctg cggctgcggc cgatcgccct gcagttgccg
     1981 gcgagcaccg ccgtcgcgct gacgaccacg cacctgcgca accgcgtcgc gtcggcctac
     2041 ttcgccgccg agccctggct cacgccgagc gcgctgagtg ccgccggcct cgacagcgac
     2101 ctgtggtgca acccggcgag actctatggc cgcgacgagg tcgtcatcgg cctgctggtg
     2161 cgcgagggca ccgccacgcg cgtgatcgac gcctggagcg gccgccgcga gcgaatggac
     2221 gcccaggcac gcggctactg gcagggccgc atggcgatgc ggccatggaa cgtgttcgtc
     2281 gcgcaggtgc tgcagttcca gtgccagctc agcggcctgt tcgacgccag cagtggattg
     2341 acaggcggcg gcgactgcga gcgcatccgc cggctgctcg attccacgcg catgcagatc
     2401 gatgcgctgc acaggcgcta cagcgaaggc gccctgcgca tcctgcagaa gttcggcaac
     2461 aagccgaccg cggcggatgc gcagagcatg gtcaacgagc tcaagggctc gtacgcggat
     2521 ctgtacgaga tctcgcaggg cctggccggc gccgacgtcg gcgccggcgc gctgccggtc
     2581 gtccgcatgc tgctgaacgc aggcttcgtc gagctgccgc cggccggata cctccccgtc
     2641 gtaccgggcc agcagcccct ggccgcgcag ctgtcgcgca tgttcggcga aggcgtcgcg
     2701 ctgactttcc acgccgtgcg ccacgacgag atcgcgcacc ttgtcgagga agcgcagcac
     2761 ctcgatcgca tctcgctcac ccgcgggctc gacgacccgg cccaggtcga gcaggtcgaa
     2821 gtcttcgtgc ccgacggcac gcagaccgac gccgccacgc aggcccccgg gcgctggtgg
     2881 caggcggcgc ttcaggtcga cgcactgggc gacgtcgacc tcggcctcga gcgctcgacc
     2941 tatacgctcg cgcccgagcg gacgaccgcg ggcggcggac aggccacgga cgtgcggccg
     3001 gtcacgggca tgctgggcct gcgatcggcg agggcaagcg cccccgcagc gcaggcgccg
     3061 gcacctgcgt ccgcgaagaa gcggacggcg gtcggcgcgc agcaggtcgc cgcccccccg
     3121 gcgcaatcca tcggcacggc gaggccg
//
LOCUS       DASVLN010000897         3490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_101584, whole genome shotgun sequence.
ACCESSION   DASVLN010000897 DASVLN010000000
VERSION     DASVLN010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101584"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VF453_21085"
     CDS             <1..250
                     /locus_tag="VF453_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690214.1"
                     /translation="VPALVNGVAGQPRRERAVVTERVPAADDPACPHRLAVASASIER
                     PMDQCVAPDVWAGAQVGGHVTLELRASGVGAEIVAVRP"
     gene            331..2436
                     /gene="ppk1"
                     /locus_tag="VF453_21090"
     CDS             331..2436
                     /gene="ppk1"
                     /locus_tag="VF453_21090"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX7690215.1"
                     /translation="MTDVSLAAAPPVRLALLNRERSILEFNRRVLAQALRPDVPLLER
                     LRYVTIVSSNLDEFFEVRFASALEAARAPGAGDAWSHLVSVAEEAHVLIQEQYAVFNE
                     QVMPALAVHGIRIVNHSERGVAQREWVAKFFEREVRPLLLPVGLDPAHPFPQVANKSL
                     NFIVQLGGRNAFGRENTIAIVKVPRVLPRVIKLPDACCPKGEQHFVLLSSVIRAHLAE
                     LFPGRVVESFSQFRVTRDSELEVDEDDIRNLRQALRRGLTTRHYGRAIRLEVVNTCPE
                     ALWRLLLAQFDLPEAALHRVNGPVNLVRLNELIDQAVDPRHGPVLRFPPYEPSWPLGR
                     LPQSGSIFDRLRDGGDVLLHHPFESFEPVVEFLREAVLDPQVLAIKQTIYRTGEKSVL
                     MDLLIEAARRGKEVLAVVELKARFDEEANINWAERLEAVGAQVVYGIVGLKTHAKLLL
                     VTRREDSKHGARLQRYVHLSTGNYNPKTARLYTDLGYLSADEGLTSDADQVFQQIASL
                     TRMRPPRHLLAAPFVLHKRMLAHIAQVEAAARAGKPARIVVKVNALTDTALIEALVNC
                     GQAGASIDLVIRGACSLPPGIEGLTANIRVRSVVGRFLEHSRIVYFRWGESDDEEALY
                     LSSADWMLRNMLRRIELAWPIRDPALRQRVIDECLVPYLHDTRDAWTLGADGRYTRVG
                     EDGVSAQQALMARFRKRAE"
     gene            2472..2948
                     /locus_tag="VF453_21095"
     CDS             2472..2948
                     /locus_tag="VF453_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7690216.1"
                     /translation="MDLILWRHADAGDNLPDPVADLERPLRPKGERQARRVADWLNQF
                     LPDSARVLTSPALRCTQTAAALGRRVKKVAALGPDGDVEGLLAAARWPDAKEAVLVVG
                     HQPTLGMAAAYLMSGEAARFGEPWSIKKGSVWWLRHRLRDNRSEVQIVAVRTAEGL"
BASE COUNT          569 a         1174 c         1195 g          552 t
ORIGIN      
        1 ggtgccggcg ctggtcaacg gcgtcgccgg ccagccgcgt cgcgagcgcg ccgtcgtgac
       61 cgagcgcgtg cccgccgccg acgatcctgc ctgcccgcac cgcctcgcgg tcgcgagcgc
      121 ctcgatcgag cggccgatgg accagtgcgt cgcgcccgac gtctgggccg gagcgcaagt
      181 cggcggccac gtgacgctgg agctgcgcgc gagcggcgtc ggcgccgaga tcgtcgccgt
      241 ccgcccctga cgaccggcgc tcgactcagc tagggcaaac cagcccggcc gtgacgcgaa
      301 actgtcatca aatgttcata aactgagcgg atgaccgacg tttccctggc tgcggcgccg
      361 ccggtgcgcc tggccctgct caatcgcgag cgatcgatcc tcgaattcaa ccgccgcgtc
      421 ctcgcccagg cgctgcggcc cgacgtgccg ctgctggagc ggctgcgcta cgtcaccatc
      481 gtctcgtcca atctcgacga gttcttcgag gtgcgcttcg cgagcgcgct cgaggccgcg
      541 cgcgcacccg gcgcggggga cgcctggagc cacctggtga gcgtcgccga agaggcgcac
      601 gtcctgatcc aggagcaata cgcggtcttc aacgagcagg tgatgccggc gctggccgtg
      661 cacggcatcc gcatcgtcaa tcactcggag cgcggcgtcg cgcagcgcga gtgggtcgcg
      721 aagttcttcg agcgcgaggt gcggccgctg ctgctgcccg tcggcctgga tccggcgcac
      781 ccgttccccc aggtcgccaa caagtcgctg aacttcatcg tgcagctggg cggacgcaac
      841 gcgttcgggc gcgagaacac gatcgccatc gtcaaggtgc cgcgcgtgct gccgcgcgtg
      901 atcaagctgc ccgacgcctg ctgcccgaag ggggagcagc acttcgtcct gctctccagc
      961 gtgatccgcg cccacctggc ggagctgttc ccggggcgcg tcgtcgaatc gttctcgcag
     1021 ttccgtgtga cgcgcgactc cgagctcgaa gtcgacgagg acgacatccg caatctgcgc
     1081 caggcgctgc gccgcgggct gacgacgcgg cattacggac gcgcgatccg gctcgaggtc
     1141 gtcaacacct gccccgaggc gctgtggcgg ctgctgctcg cccagttcga cctgcccgag
     1201 gcggcgctgc atcgcgtcaa cgggccggtc aacctcgtgc gcctgaacga gctgatcgac
     1261 caggccgtgg accccaggca cggcccggtg ctgcgcttcc cgccctacga gccgtcctgg
     1321 ccgctgggcc ggctgccgca atcgggctcg atcttcgacc gcctgcgcga cggcggcgac
     1381 gtgctgctgc accatccgtt cgagagcttc gagccggtgg tggagttcct gcgcgaggcg
     1441 gtgctcgacc cgcaggtgct cgcgatcaag cagacgatct accgcaccgg cgagaagtcg
     1501 gtgctgatgg accttctgat cgaggcggcg cgccgcggca aggaggtgct ggccgtcgtg
     1561 gagctcaagg cgcgcttcga cgaggaggcc aacatcaatt gggcggagcg gctggaggcc
     1621 gtcggcgcgc aggtggtcta cggcatcgtc gggctgaaga cgcatgccaa gctgctgctc
     1681 gtgacgcggc gcgaggattc caagcacggc gcgcgcctgc agcgctacgt ccacctgtcg
     1741 acgggcaact acaacccgaa gacggcgcgt ctgtacaccg acctcggcta cctgagcgcc
     1801 gacgagggcc tgacctccga cgccgaccag gtcttccagc agatcgcgag cctcacgcgc
     1861 atgcgcccgc ccaggcacct gctggccgcg ccgttcgtgc tgcacaagcg gatgctggcg
     1921 cacatcgcgc aggtcgaggc cgccgcgcgc gcgggcaagc cggcgcgcat cgtggtcaag
     1981 gtcaatgcgc tgaccgacac cgcgctgatc gaagcgctcg tcaattgcgg ccaggcgggc
     2041 gcgagcatcg acctcgtcat tcgcggcgcg tgctccctgc cgcccggcat cgagggcctg
     2101 acggccaaca tccgcgtgcg ctcggtggtc gggcgctttc tggagcactc gcgcatcgtc
     2161 tacttccgct ggggcgagtc ggacgacgag gaggcgctct atctctcgag cgccgactgg
     2221 atgctgcgca acatgctgcg gcgcatcgag ctcgcgtggc cgatccgcga tccggcgctg
     2281 cgccagcgcg tgatcgacga atgcctggtc ccctatctgc acgatacgcg cgacgcctgg
     2341 acgctgggcg ccgacggccg ctacacgcgc gtcggcgagg atggcgtcag cgcgcagcag
     2401 gcgctgatgg cacgctttcg caagcgcgcg gaatagggtg cagcccgcac gaccatccag
     2461 gagcccgtga catggacctg atcctctggc gccacgccga tgccggcgac aacctgcccg
     2521 acccggtggc ggacctcgag cggccgctgc gtcccaaggg cgagcgccag gcgcgccgcg
     2581 tcgccgactg gctcaaccag ttcctgccgg actcggcgcg cgtgctcacg agcccggcgc
     2641 tgcgctgcac gcagacggcg gcggcgctcg gccggcgcgt caagaaagtg gcggcgctcg
     2701 ggccggatgg cgatgtggag ggcctgctgg ccgcggcccg ctggcccgac gccaaggagg
     2761 cggtcctggt cgtcggccat cagccgacgc tcggaatggc ggccgcctac ctgatgtcgg
     2821 gcgaggcggc gcgcttcggc gagccgtggt cgatcaagaa gggcagcgtc tggtggctgc
     2881 gccatcgcct gcgcgacaac cgctccgagg tgcagatcgt ggccgtgcgc acggccgagg
     2941 ggctctgacg ccgtctctcg tctcgcaggg gtcgccgcgg ccctttccga aaatctttcg
     3001 gcaagtgctt gcgctccgtg aagcggcgcg tatagaatgg caggcttcgc tgatcgcaag
     3061 tcagcgccga ccgcagcgtg gcgggcaagc cgaaaggttt ctcgtgcgca agtttggcga
     3121 ccgacgggtc tgcgaagaaa gtctgaaatt gatgcttgac gacgattcgg aatcgagcat
     3181 agaataacag gcttcgctga tcgaaagttt gttggcaaat aacgcgacaa acaggcgacg
     3241 gcgaaacaag cgagaaagtc gactgcttga caaggtttta aaagcagatc ataatctcgc
     3301 ttctgcgctg aaaacagcgc gacgcagcaa acaacggttt gttcgcggta agttctttaa
     3361 aaatcaacag ccgataagcg tgggcgtttg aaggcgagtg ccaagagtcc ttcgggattc
     3421 gaagtcatat gactttaaac gctcatggaa attgaagata gcttgcaaaa gtttcttcga
     3481 ttcctttatg
//
LOCUS       DASVLN010000898         2869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_477895, whole genome shotgun sequence.
ACCESSION   DASVLN010000898 DASVLN010000000
VERSION     DASVLN010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477895"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..553)
                     /locus_tag="VF453_21100"
     CDS             complement(137..553)
                     /locus_tag="VF453_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX7690217.1"
                     /translation="MNPIDEVLYTGRTRTVGGRDGSARSDDARLDIRLSPPGSTGPGT
                     NPEQLFAAGWSACFIGAMGRAARAAGIALPTGVAVDAEVDLCKAGEGFVLQARLKVSL
                     PGLAPEVARSVVDGAHQLCPYSRATRGNIAVELSIA"
     gene            698..1159
                     /locus_tag="VF453_21105"
     CDS             698..1159
                     /locus_tag="VF453_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7690218.1"
                     /translation="MPTRPKAPDLPRLGDFLCFAVYSANLAYGRAYKPILDELGVTYT
                     QWIAIVALWEEDGQTVGSLGEKLFLESNTLTPILKKLEALGYVERRRDPGDERQVIVR
                     LTDAGRALREKGAQRTLVKATGLAADEFAKTRRAMNEVRDNLIRHAQRSQD"
     gene            1290..1985
                     /locus_tag="VF453_21110"
     CDS             1290..1985
                     /locus_tag="VF453_21110"
                     /inference="COORDINATES: protein motif:HMM:NF019913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690219.1"
                     /translation="MKLSSTAAGLALALAAVSAAAATKVGPFTVYGDIEKRWLSLGGT
                     RSPLGPPTSDEHDQIGHGRVQRFANGVISWTPEAGAAQRLIGGESTTFDTGYVPSSLP
                     MGGFMRLTVHRNGDYELTVHAHDSGATNIGYGFTAVLVGADGQPFTFTHTGHLQGTTG
                     AVFGHPKRSDDFVTKGHDNRLAAHYPQLVETGSFLGTLTGQDKLRQGLQDLLEDAAKS
                     LAAKGVQAVIALV"
     gene            2087..2647
                     /locus_tag="VF453_21115"
     CDS             2087..2647
                     /locus_tag="VF453_21115"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX7690220.1"
                     /translation="MGEVTQLIARAREGDRPSLDRLFEVLYPELRRIAHRRLWHGGRG
                     GAFETTALVNECYLKFVQRDGLGASDRAHFLAYAAAVMRSVIVDAARSARADRRGGDL
                     QHVTLDTAAGAAAAESFAADELLDIHAALEDLQRIDPRLVQVVEMRFFAGMTDAEIAQ
                     VLGLTDRTVRRDWEKARLLLAHSLRG"
     gene            2674..>2869
                     /locus_tag="VF453_21120"
     CDS             2674..>2869
                     /locus_tag="VF453_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690221.1"
                     /translation="MSSLDKLSTAWATISTLLDEALALPAGERAAWLDALAGERAAHR
                     ETLLALLRTQADVERDDAFLR"
BASE COUNT          427 a         1016 c         1027 g          399 t
ORIGIN      
        1 cggcggcgag ggcggcgagc gtcgcgctgc cgagcaggcg gcggcgagtc gggtcggcga
       61 gggtggaatc ggcgtgcgtc atggcgtgcg tccttggtcg ggtgtgtgtc ggcgcgagga
      121 gtgcgccgca ggcggatcag gcgatggaga gctcgacggc gatgtttccg cgcgtcgccc
      181 tggagtacgg gcacagctgg tgggcgccgt cgacgacgct gcgcgcgacc tccggcgcga
      241 ggccgggcag gctgaccttc aggcgcgcct gcagcacgaa gccctcgccg gccttgcaga
      301 ggtcgacctc cgcatccacc gcgacgccgg tgggcagggc gatgccggcg gcgcgcgcgg
      361 cgcggcccat cgcgccgatg aagcaggccg accagccggc cgcgaacagc tgctccgggt
      421 tcgtgcccgg gccggtgctg cccggcggcg acaggcggat gtcgaggcgc gcgtcgtcgc
      481 tgcgcgccga tccgtcgcgg ccgccgacgg tgcgggtgcg gccggtgtag aggacttcgt
      541 cgatgggatt catggtcggt tcctggctgg ttgaaatcgc atgtgaatta atcgcatgcg
      601 atgcacttta cggatcggat tcggtgatgt caatgtctgc gatcaaatcg catgcgatta
      661 aaaagggata tcatcggatc ccaaggagac cgcgaccatg cctacccgtc ccaaagcgcc
      721 cgatctgccc aggttgggcg acttcctctg cttcgccgtc tactccgcca acctcgcgta
      781 cggccgcgcg tacaagccca tcctcgacga gctgggcgtc acctacacgc agtggatcgc
      841 catcgtggcg ctgtgggagg aggacggcca gaccgtcggc agcctcggcg agaagctgtt
      901 cctcgagtcc aacacgctca cgcccatcct caagaagctc gaggcgctgg gctacgtcga
      961 gcgccgccgc gatccgggcg acgagcgcca ggtgatcgtc cgcctcaccg acgccggccg
     1021 cgcgctgcgc gagaagggcg cgcagcgcac gctcgtcaag gcgacgggcc tcgccgcgga
     1081 cgagttcgcg aagacgcggc gcgcgatgaa cgaggtgcgc gacaacctca tccggcacgc
     1141 gcagcggtcg caggactgaa ggcggcaacc cctagtttga cggcgggcgg catgtccgcc
     1201 gaagccgcgg aggtgcgtat caacgggtga gtgatcgcga ggtctgcacg cagcgaccgc
     1261 tcgcctgtcc aacccacccc gaggtttcca tgaagctctc ttccactgca gccggcctcg
     1321 cgctcgcgct ggccgccgtc tcggcggccg cggccaccaa ggtcggtcct ttcaccgtgt
     1381 acggcgacat cgagaagcgc tggctctcgc tcggcggcac ccgcagcccg ctcggcccgc
     1441 cgacctccga cgagcacgac cagatcggcc acggtcgcgt gcagcgcttc gccaacggcg
     1501 tgatcagctg gacgcccgag gcgggcgcgg cgcaacgtct gatcggcggc gagtcgacga
     1561 ccttcgacac cggctatgtc ccgagcagcc tgccgatggg cggcttcatg cggctgaccg
     1621 tgcatcgcaa cggcgactac gagctcaccg tgcatgccca cgacagcggc gccaccaaca
     1681 tcggctacgg cttcaccgcg gtgctcgtgg gcgccgacgg ccagcctttc acgttcacgc
     1741 acaccggcca cctgcagggc acgaccggcg ccgtcttcgg ccatccgaag cgcagcgacg
     1801 acttcgtgac caagggccac gacaaccggc tggccgcgca ctacccgcaa ctggtggaga
     1861 ccggcagctt cctgggcacc ctcaccggcc aggacaagct gcgccagggc ctgcaggacc
     1921 tgctcgagga cgcggccaag agcctggccg ccaagggcgt gcaggccgtc atcgcgctcg
     1981 tgtgaccggg cccgcgctcg ccctgcccac gggccgccgg tcggccgcgg gcgccgcccc
     2041 ccgtcgctat gatccgctcc acatcggggt ggcaaggagc gacgacatgg gcgaggtcac
     2101 gcaactgatc gcgcgggcgc gggaaggcga ccgcccgtcg ctggatcgcc tgttcgaggt
     2161 gctgtacccg gagctgcggc gcatcgccca tcggcggctc tggcatggcg gccggggcgg
     2221 cgcgttcgag acgaccgcgc tcgtcaacga gtgctacctg aagttcgtgc agcgcgacgg
     2281 cctcggcgcg tccgatcgcg cccatttcct cgcctacgcg gcggccgtga tgcgctcggt
     2341 catcgtcgac gccgcccgca gcgcccgcgc ggaccgtcgc ggcggcgacc tgcaacacgt
     2401 cacgctggac accgctgcgg gcgccgccgc ggccgaaagc ttcgcggccg acgagctgct
     2461 ggacattcac gcggcactcg aggatctgca gcggatcgac ccgcggctgg tgcaggtcgt
     2521 cgagatgcgc ttcttcgccg gcatgaccga cgcggagatc gcgcaagtgc tcgggctgac
     2581 cgaccgcacc gtgcgccggg actgggagaa ggcgcggctg ctgctcgcgc actcgctgcg
     2641 cggctgacgg cgctcgcccg gagggagatg gcgatgagtt ccctggacaa gctcagcacc
     2701 gcctgggcga cgatcagcac gctgctggac gaggcgctcg cgctgccggc cggcgagcgc
     2761 gccgcctggc tcgatgcgct ggccggcgag cgcgccgcgc atcgcgagac gctgctcgcg
     2821 ctgctgcgga cgcaggccga cgtggagcgc gacgacgcgt tcctgcgcg
//
LOCUS       DASVLN010000899         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_101632, whole genome shotgun sequence.
ACCESSION   DASVLN010000899 DASVLN010000000
VERSION     DASVLN010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101632"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..586
                     /locus_tag="VF453_21125"
     CDS             50..586
                     /locus_tag="VF453_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690222.1"
                     /translation="MAPTARHRLARALRAVPPFVLAGACAAIAVPALAAKPCEAVAEQ
                     LNELASYKETNKALLRNYQDCLAVSEVCSLTQEHDGTDGMVLSGTASGLVDDGLDRSS
                     VMTAYQDERTGTCLFAIHTGGSAASWLFHGWSVRGGRAEPIAQMERFSHRADFESTHA
                     LVGLLGQARAWAVARTGQ"
     gene            1080..2378
                     /locus_tag="VF453_21130"
     CDS             1080..2378
                     /locus_tag="VF453_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEX7690223.1"
                     /translation="MPLNDDTARQRKMQLLAALSAAVAAGAPLTAAANPPNCSAWNAS
                     TAYTAGAVVVDQSKTYTANWWTQGQEPVNNNGGAGSGQPWTLSSGCSGSPSPSPSPSP
                     SPSPSPSPSPSPSPSPSPSPSPAGATFFGPYKDITVSMNWNTNVISTAVTGTLSPVLS
                     VKPAKLKEITWAFATGACGSENWGGLQASAVAAANVQNFVNAGVKYVISTGGAAGSFT
                     CGNDTDFETFVNRYASANLAGIDFDIEAGQTQADIDNLVARVKASQSKHPGLRWSFTL
                     ATLGGNSAQSLGSMGVNVMNSIKSHGLTNYIVNLMTMDYGSAIASNCTLNSSGKCDMG
                     KSAIQAAVNLHNYWGVPYSQIELTPMIGGNDATDEVFSIADAATVSSWVKANGAAGIH
                     FWSLDRDKDCAPGYASATCNSYGSAGTWGFTNAFISDLGL"
BASE COUNT          395 a          897 c          767 g          346 t
ORIGIN      
        1 cgcgcgcctg cggccccccg ggccgagccc tgccaacgac acgagaccca tggcccccac
       61 cgcccgtcac cgccttgcgc gcgccctgcg cgccgtcccg cccttcgtcc tggccggcgc
      121 ctgcgccgcc atcgccgtgc ccgcgctcgc cgccaagccc tgcgaggcgg tcgccgagca
      181 actgaacgag ctcgcgtcgt acaaggagac gaacaaggcg ctgctgcgca actaccagga
      241 ctgcctcgcg gtctcggagg tgtgctcgct cacgcaggag cacgacggca ccgacggcat
      301 ggtgttgagc gggacggcct cgggcctcgt cgacgacggc ctcgaccggt cgtcggtcat
      361 gaccgcctac caggacgaac gcacgggcac ctgcctgttc gcgatccaca ccggcggctc
      421 ggcggcgtcc tggctgttcc acggctggtc cgtgcgcggc ggccgtgccg agccgattgc
      481 gcagatggag cgcttctcgc atcgcgccga cttcgagtcg acgcacgcgc tcgtcggcct
      541 gctcgggcag gcgcgggcct gggcggtggc gcgcaccggc cagtaggcgc gcggcgatcg
      601 atccccgtgc cacggggccg catcgcgagg gatcgcgacg cgtctcgcat ggcccctcaa
      661 gcatgaccac ttaagaccag tggtctatgt cgtcgccgcc atggtctgca agccgatcgg
      721 cagccggctc gggcgcgacg ctcgattcgg acccgcgatt ccttcggcct cttcgctcgc
      781 aagccgcctg atccgacgat tcgatcaggt tggcgcgcgc tccgcgatgg gacacggaat
      841 cgatccctat cgaagtgagc gccaacgatt cgagattcgc ttcactcaac tggttctgta
      901 gtggtaatta ccatgtcaag cacatgacag ccggattacg ctgcccgcga tccgaagaga
      961 gttgcggtgc gtccgtcccg aagatcgatc ccagcggctg ccgtcctcgc ggattcccgt
     1021 cgacgccctc ctccccctga aggcgccttt cgaacccggc aaagcacacg gagaacgaca
     1081 tgcccctgaa cgacgacacc gcgcgccagc gcaagatgca gctgttggcc gccctctcgg
     1141 ccgccgtcgc ggccggcgcg cccctgacgg ccgccgccaa cccgcccaac tgctcggcct
     1201 ggaacgcctc gaccgcgtac accgccggcg cggtggtggt cgaccagtcc aagacctaca
     1261 ccgccaactg gtggacgcaa ggccaggagc ccgtgaacaa caacggcggc gccggcagcg
     1321 gccagccgtg gacgctttcc agcggctgca gcggctcgcc ctcgccgagt ccttcgccgt
     1381 cgcccagtcc gtcgccgtcg ccgagcccca gcccgtcgcc ctcgccttcc ccgagcccgt
     1441 cgccgtcgcc cgccggcgcg accttcttcg gcccgtacaa ggacatcacc gtcagcatga
     1501 actggaacac caacgtcatc tcgacggcgg tgacgggcac gctgtcgccg gtgctgagcg
     1561 tcaagccggc caagctgaag gagatcacct gggccttcgc gaccggcgca tgcggcagcg
     1621 agaactgggg cggcctgcag gcctccgcgg tggccgcggc caacgtgcag aacttcgtca
     1681 acgcgggcgt gaagtacgtg atctcgaccg gcggcgccgc gggctcgttc acctgcggca
     1741 acgacaccga cttcgagacc ttcgtcaatc gctacgcctc ggccaacctc gccggcatcg
     1801 acttcgacat cgaggccggc cagacccagg ccgacatcga caacctggtc gcgcgcgtca
     1861 aggcctcgca gtccaagcat ccgggcctgc gctggagctt cacgctggcc acgctgggcg
     1921 gcaactcggc gcagagcctg ggctcgatgg gcgtcaacgt gatgaactcg atcaagtcgc
     1981 acgggctgac caactacatc gtcaacctga tgacgatgga ctacggcagc gcgatcgcgt
     2041 cgaactgcac gctgaactcg agcggcaagt gcgacatggg caagtcggcg atccaggcgg
     2101 cggtcaacct gcacaactac tggggcgtgc cgtactcgca gatcgagctg acgccgatga
     2161 tcggcggcaa cgacgcgacg gacgaggtgt tctccatcgc cgacgcggcc acggtgagca
     2221 gctgggtcaa ggccaacggc gccgcgggca tccacttctg gtcgctcgac cgggacaagg
     2281 actgcgcgcc cggctacgcc tcggccacct gcaacagcta cggctcggcc ggcacctggg
     2341 gcttcacgaa cgcgttcatc tccgacctgg gactctgaac gcccacgacg gcgcacgccc
     2401 cgcgc
//
LOCUS       DASVLN010000900         7980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_369677, whole genome shotgun sequence.
ACCESSION   DASVLN010000900 DASVLN010000000
VERSION     DASVLN010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369677"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VF453_21135"
     CDS             <1..1282
                     /locus_tag="VF453_21135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEX7690224.1"
                     /translation="APASAPVRAAAPARPASVPAPQPQEESLLSTITDSPFVLPGIGV
                     LVALIGGFAFWKKRQGKRSFSGETSFIESKIQPDSFFGASGGQRVDTRDGGNSSSASS
                     SLNYSLSQLDAIGDVDPVAEADVYLAYGRDLQAEEILKEAQRSDPDRLAIRTKLLEVY
                     AKRADLKTFETQARQLQALTGGRGEDWAKAQELGRQIDPTNPLYQEGSEISMADTEPA
                     ALTPLVPHDDAEQTLMRPPTHPATEQPTPTDFSTTDLPIDFDGDHEPTTTRQGFESTA
                     EVPMEHAAPAPVPAVEEVPFEVSDFDLGTEPVELAAPAPAARNEHLQDVSVSPPPSID
                     FDFGDLSLDLDKPAAAAAVEHAPAVEHASDLPELEMPAAAADSEPMARKIELADEFRR
                     IGDHEGARELLEEVVSKADGPLKSRAQTMLDALG"
     gene            1548..2387
                     /gene="truA"
                     /locus_tag="VF453_21140"
     CDS             1548..2387
                     /gene="truA"
                     /locus_tag="VF453_21140"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX7690225.1"
                     /translation="MALGIAYRGGGYHGWQSQPDGRTVQDLLERALGEFVGLPKGGLV
                     PTVCAGRTDAGVHALNQVVHFDAPVEREAFSWVRGTNRFLPPDVAVQWARAVEPDFHA
                     RNHARGRSYAFLVRESAVRPAIEAGMCGWVFRPLDADAMRTAAQALLGEHDFSSFRAS
                     MCQALSPVKQLRRLDIARIGDYWRFDFEASAFLHHMVRNIMGALVAIGSGSLPATAMA
                     ELLAARDRSAAPPTFPADGLYFLGPRYDVRLGLPERTTAHDWLPQLPPGHSSPDEAYP
                     PRP"
     gene            2468..3124
                     /locus_tag="VF453_21145"
     CDS             2468..3124
                     /locus_tag="VF453_21145"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX7690226.1"
                     /translation="MTAPAHRTRIKICGLTREQDVDAAVEAGADAVGFVLYERSPRHV
                     DVARLAVLARRLPPFVAPVCLFVNASPDLIDAAVAAVPQALLQFHGDEDPQQCEQPGR
                     PYLRAVRMAPGVDLLDYASRFASASGLLLDAFVEGYGGGGKVFDWSQIPRGVPRPVVL
                     SGGLNPANVIDGVMRVRPWAVDVSSGVESAKGVKDAALVRRFCEAVREADARAADAPL
                     "
     gene            3219..4478
                     /gene="trpB"
                     /locus_tag="VF453_21150"
     CDS             3219..4478
                     /gene="trpB"
                     /locus_tag="VF453_21150"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX7690227.1"
                     /translation="MSDYSQPDATGHFGPYGGTFVAETLVHAIAELKDAYAHYRVDAE
                     FQREFRHELAHFVGRPSPVYHAARLSRENGGAQIWLKREDLNHTGAHKVNNTIGQVLL
                     ARRMGKPRIIAETGAGQHGVATATICARYGLECVVYMGAEDVKRQSPNVYRMHLLGAK
                     VVPVDSGGRTLKDALNEALRDWVTNVENTFYVIGTVAGPAPYPEMVREFQSVIGEECL
                     QQMPGEIGRQPDAVVACVGGGSNAMGIFYPYIGHAATRLIGVEAAGLGLETGKHAASL
                     SAGSPGVLHGNRTYLLQDANGQILETHSISAGLDYPGVGPEHAYLKDIGRAEYVGITD
                     KEALEAFHRLCRTEGIIPALESSHAVAHAIKLAATMKPDQHVLVNLSGRGDKDIGTVA
                     DLSGADFYCRPSCRGQSVKGGPVGGAQ"
     gene            4475..5290
                     /gene="trpA"
                     /locus_tag="VF453_21155"
     CDS             4475..5290
                     /gene="trpA"
                     /locus_tag="VF453_21155"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7690228.1"
                     /translation="MSSRIDTVFARLREQGRKAVIPYVCCGDPFADATVEILLGLAAG
                     GADVIELGVPFSDPMADGPVIQKASERALARGIGNDQVLAAVRGFRERDTTTPIVLMG
                     YANPVERYDLVHGAGAFVREAAAAGVDGVLVVDYPPEESQAFSASLKAAGLAPIFLLA
                     PTSTEARMRQVGEIAAGYVYYVSLKGVTGAGHLDTAAVAAAVPRIKAHVRVPVGVGFG
                     IRDGATARAVAQVADAVVIGSALVQCLEAQTRAGVAEAARRFVAEIRSAVDGA"
     gene            5428..6300
                     /gene="accD"
                     /locus_tag="VF453_21160"
     CDS             5428..6300
                     /gene="accD"
                     /locus_tag="VF453_21160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX7690229.1"
                     /translation="MSWLEKLLPPKIQQTDPSERRTIPEGLWIKCPSCETVLYKTDLE
                     QNLNVCPKCDHHLRIGARARLEAFLDAEGRYEIGQEVLPVDPLKFKDSRKYPERLQGA
                     LEATGETDALVVMGGSVMSVPVVVACFEFDFMGGSMGSVVGERFVRGVRAAVEQRVPF
                     VCYTATGGARMQEGLFSLLQMAKTNGALTLLSKAKLPYISVLTDPTMGGVSASFAFIG
                     DVVIAEPKALIGFAGPRVIENTVREKLPEGFQRSEFLLKKGAIDLICDRRQLRPTIAR
                     LLAQLQRQSADAVA"
     gene            6480..7790
                     /gene="folC"
                     /locus_tag="VF453_21165"
     CDS             6480..7790
                     /gene="folC"
                     /locus_tag="VF453_21165"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEX7690230.1"
                     /translation="MTHPRTLAEWLAHCERLHPKTIDMTLSRVHKMVERLQVRFEAPV
                     ISVAGTNGKGSTCAMLDAIARAAGYRVGLFSKPHLVHFEERCRIDGAMITEAELLPHF
                     EAVERARGDLQLTYFEFTTLAILHALAATPLDLVILEVGLGGRLDAVNGIDADVAVVT
                     SIDIDHVEYLGPDRESIGREKAHIARPGRPLIVGDPMPPASIQAYAESIGADLWLQGR
                     DFNYSGDRQQWSWSGRGRRWNALGYPALRGANQLLNASGALAALEALRERLPVTAQAV
                     RVGLASVELPGRFQIVPGQPALVLDVAHNPHSVAALALNLDQMGFFPRTFAVFGAMHD
                     KDLAGAVGKIVHLVDDWHVTDLPLARAAKAAELAETIWALTAAQPPSAPKPRVTTHRS
                     PQEALAAAMSQADPADRIVVFGSFYTVGGVLKDGLPRGVARHVG"
BASE COUNT         1130 a         2925 c         2826 g         1099 t
ORIGIN      
        1 cgcgccggcg tccgcgcccg tgcgcgccgc ggcgcccgcg cgtccggcct cggtgcccgc
       61 gccccagccg caggaagaga gcctgctgtc cacgatcacc gactcgccgt tcgtcctccc
      121 gggcatcggc gtcctggtcg cgctcatcgg tggcttcgcg ttctggaaga agcgccaggg
      181 caagcgcagc ttctccggcg agacctcgtt catcgagagc aagatccagc ccgactcgtt
      241 cttcggcgcc agcggcggcc agcgcgtcga cacgcgcgat ggcggcaaca gctcgtcggc
      301 gtcgtcgtcg ctgaactact cgctgagcca gctcgacgcc atcggcgacg tggatcccgt
      361 ggccgaggcc gacgtctacc tcgcctacgg ccgcgacctg caggccgagg agatcctgaa
      421 ggaagcccag cgctcggatc cggaccgcct cgcgatccgc acgaagctcc tcgaggtcta
      481 cgccaagcgc gccgacctga agaccttcga gacccaggcg cgccagctcc aggccctgac
      541 cggcgggcgc ggcgaggact gggcgaaggc gcaggagctc ggccgccaga tcgacccgac
      601 gaatccgctg taccaggaag gcagcgagat cagcatggcc gacacggagc ccgcggccct
      661 cacgccgctg gtgccgcacg acgatgccga gcagacgctg atgcgcccgc cgacccatcc
      721 ggcgaccgag cagccgacgc cgaccgactt cagcacgacc gacctgccga tcgacttcga
      781 cggagaccac gagccgacca cgacccgcca gggcttcgag tccaccgccg aagtgccgat
      841 ggaacacgcg gcgccggcgc cggtgcccgc cgtcgaggaa gtgccgttcg aggtcagcga
      901 cttcgacctc ggcaccgaac cggtggagct cgccgcgccg gcccccgccg cgcgcaacga
      961 gcacctgcag gacgtgtcgg tcagcccgcc gccgtcgatc gacttcgact tcggcgacct
     1021 gtcgctcgac ctcgacaagc ccgccgccgc ggcggccgtc gagcatgcgc cggcggtcga
     1081 gcacgcgagc gacttgccgg agctcgagat gccggccgcc gccgcggaca gcgagccgat
     1141 ggcgcgcaag atcgagctgg ccgacgagtt ccgccgcatc ggcgaccacg agggtgcgcg
     1201 cgagctgctc gaggaggtcg tctccaaggc cgatggcccc ttgaagtcgc gggcacagac
     1261 gatgttggac gcgttggggt aacctcgcgc gggtgacgtc caccgaccgc ttccccagtc
     1321 cgccggccga agggcccggc ggccacacga gcccgggccc tgcgcccggg tcttcttttg
     1381 cgcccggcgg cacgaccctg ccgacggccg ccgcaagcgg cgcgacgggc gtgccgggcc
     1441 attcgtcgga aggcccgccg gtgaccgccc tcgaggccac cggccgcgcc gacgacgagc
     1501 cttcgccgca cgaggcgccg cccgggccgg gcctcggcac cggccgcatc gcgctgggca
     1561 tcgcctaccg cggcggcggc taccacggct ggcaatcgca gccggacggg cgcaccgtgc
     1621 aggacctgct cgagcgcgcg ctcggcgaat tcgtcggact gcccaagggc gggctcgtgc
     1681 ccaccgtctg cgccggccga acggacgcgg gggtgcatgc gctcaaccag gtcgtgcatt
     1741 tcgacgcgcc ggtggagcgc gaggcgtttt cctgggtgcg tggcaccaac cgcttcctgc
     1801 cgccggacgt cgccgtccag tgggcgcggg ccgtcgagcc cgacttccac gcccgcaacc
     1861 acgcccgcgg gcgctcgtac gccttcctcg tgcgcgagtc ggccgtgcgg ccggcgatcg
     1921 aggccgggat gtgcggctgg gtgttccgcc cgctcgacgc cgacgcgatg cgcaccgcgg
     1981 cgcaggcgct gctgggcgag cacgacttca gcagcttccg ggcgtcgatg tgccaggcgc
     2041 tgtcgccggt caagcaactg cgccgcctcg acatcgcgcg catcggcgat tactggcgtt
     2101 tcgacttcga ggccagcgcc ttcctgcatc acatggtgcg caacatcatg ggcgcgctgg
     2161 tggcgatcgg cagcggcagc ctgccggcca ccgcgatggc ggaactgctc gccgcccgcg
     2221 accgctcggc cgcgcctccg acctttccgg ccgacgggct gtacttcctc ggcccgcgct
     2281 acgacgtccg gctcgggctg ccggaacgca cgaccgcgca cgactggctg ccccagctgc
     2341 cgccgggaca ctcgagcccg gacgaggcct atccgccgcg gccctgatcc gtggcccgat
     2401 cgcggccgga ggggagggcg gcggtagact ggcgagccgt accgttcgcc agccaggatt
     2461 tcagccgatg accgcaccgg cccatcgcac ccgcatcaag atctgcggcc tcacccgcga
     2521 gcaggacgtc gacgcggccg tcgaggcggg cgccgacgcg gtcggcttcg tgctctacga
     2581 gcgcagcccg cgccacgtgg acgttgcgcg cctggcggtc ctcgcgcgcc gcctgccgcc
     2641 cttcgtcgcg ccggtatgcc tgttcgtcaa cgcgagcccc gacctgatcg acgcggccgt
     2701 cgccgccgtg ccgcaggcgc tgctgcagtt ccacggcgac gaagatccgc agcagtgcga
     2761 acagcccggc cggccctacc tgcgtgcggt gcgcatggcg cccggcgtcg atttgctaga
     2821 ctacgcatct cggttcgcca gcgcctcggg cctgctgctc gacgcgttcg tcgagggcta
     2881 cggcggcggc ggaaaggtgt tcgattggtc tcagattcct cgcggcgttc cccgtccggt
     2941 cgttttgtct ggtgggttga atcctgcaaa cgtgatcgat ggagtgatgc gcgtccggcc
     3001 ctgggccgtt gacgtgagct ccggcgtgga atcggcgaag ggcgtcaagg acgccgcgct
     3061 ggtgcgccgg ttctgcgagg cggtgcgcga agccgatgcg cgcgccgccg atgccccgct
     3121 ctgacgctcc cgccgccgcg cgctgacgcc agcgccgccg gcacggccgc tcctgaccgg
     3181 aagcggcggc gcgtcgcttc gacttcgaaa gagatccgat gtccgactat tcgcaacccg
     3241 acgccacggg ccacttcggc ccctacggcg gtaccttcgt ggccgagacc ctcgtccacg
     3301 ccatcgccga gctcaaggac gcctacgcgc actaccgcgt cgacgctgag ttccagcgcg
     3361 agttccgcca cgagctcgcc catttcgtcg gccggccgag cccggtctac cacgccgccc
     3421 gcctcagccg cgagaacggc ggcgcgcaga tctggctcaa gcgcgaggac ctgaaccaca
     3481 ccggcgcgca caaggtcaac aacacgatcg ggcaggtgct gctcgcgcgc cgcatgggca
     3541 agccgcgcat catcgcggag accggcgccg gccagcacgg cgtcgcgacg gcgacgatct
     3601 gcgcgcgcta cggcctcgag tgcgtcgtct acatgggcgc cgaggacgtc aagcgccaga
     3661 gccccaacgt ctaccgcatg cacctgctgg gcgcgaaggt cgtgccggtg gactccggcg
     3721 gccgcacgct caaggacgcg ctcaacgagg cgctgcgcga ctgggtcacc aacgtcgaga
     3781 acacgttcta cgtgatcggc acggtggccg gcccggcgcc gtatccggag atggtgcgcg
     3841 agttccagag cgtgatcggc gaggaatgcc tgcagcagat gcccggggag atcggccgcc
     3901 agcccgatgc ggtcgtcgcc tgcgtgggcg gcggcagcaa cgcgatgggc atcttctacc
     3961 cgtacatcgg gcatgcggcc acgcggttga tcggcgtcga ggccgccggc ctgggcctcg
     4021 agacgggcaa gcatgcggcc tcgctgtccg cgggatcgcc gggcgtgctg cacggcaacc
     4081 gcacctacct gctgcaggac gccaacggcc agatcctgga gacgcactcg atctccgccg
     4141 gcctggacta ccccggcgtc ggccccgagc atgcgtacct gaaggacatc ggccgcgccg
     4201 agtacgtcgg catcaccgac aaggaggcgc tggaggcctt ccaccggctg tgccgcaccg
     4261 agggcatcat ccccgcgctg gagtccagcc acgcggtcgc gcacgcgatc aagctggcgg
     4321 cgacgatgaa gcccgaccag cacgtgctgg tgaacctgtc cggccgcggc gacaaggaca
     4381 tcggcacggt cgccgatctc tccggagcgg acttctactg tcgaccgtcc tgtcgagggc
     4441 agagcgtcaa gggcggcccg gtcggaggtg cgcaatgagc tcgcgcatcg acaccgtctt
     4501 cgcccgcctg cgcgaacagg gccgcaaggc cgtgatcccc tacgtctgct gcggcgatcc
     4561 gttcgcggat gcgaccgtcg agatcctgct gggcctggcc gccggcgggg ccgacgtgat
     4621 cgagctcggc gtgccgttct ccgacccgat ggccgacggc ccggtgatcc agaaggcgtc
     4681 cgagcgcgcg ctggcccgcg gcatcggcaa cgaccaggtg ctcgccgcgg tgcgcggttt
     4741 ccgcgagcgc gacacgacca cgcccatcgt cctcatgggc tacgccaacc cggtcgaacg
     4801 ctacgacctc gtgcacggcg ccggcgcctt cgtgcgcgag gccgcggccg cgggcgtgga
     4861 cggcgtgctg gtcgtcgact acccgcccga ggaatcgcag gccttctccg ccagcctgaa
     4921 ggcggccggg ctcgcgccga tcttcctgct cgcgccgacc tccaccgagg cgcgcatgcg
     4981 ccaggtgggc gagatcgcgg ccggctacgt ctactacgtc tcgctcaagg gcgtgacggg
     5041 cgccggccac ctcgacaccg ccgccgtggc cgcggccgtg ccccgcatca aggcgcatgt
     5101 ccgggtgccg gtgggcgtgg gcttcggcat ccgcgacggg gcgacggcgc gcgcggtcgc
     5161 gcaggtcgcg gacgccgtcg tgatcggctc ggcgctggtg caatgcctgg aagcgcagac
     5221 ccgggccggc gtcgccgagg cggcgcggcg gttcgtcgcc gagatccgct cggcggtgga
     5281 cggcgcctga acaggcgtac agtccgggtt tcggattgaa gagccggcgt cctgcccagc
     5341 agggccgccg gcggagagcg gcccgggcat ccgggctgcc tcgaacgttg ggcggggccc
     5401 tcgcggccct cgagcaagga gtgatggatg agttggcttg aaaagctgtt gcccccgaag
     5461 atccagcaga cggatccgag cgagcggcgc acgatcccgg aagggctgtg gatcaagtgc
     5521 ccgtcgtgcg agacggtgct ctacaagacc gacctcgagc agaacctcaa cgtctgcccg
     5581 aaatgcgacc accacctgcg catcggcgcg cgcgcccggc tcgaggcctt cctcgacgcc
     5641 gagggtcgct acgagatcgg ccaggaggtc ctcccggtcg acccgctgaa gttcaaggac
     5701 agccgcaagt accccgagcg cctgcagggg gccctggagg ccacgggcga gaccgacgcc
     5761 cttgtcgtga tgggcggctc ggtgatgagc gtgcccgtcg tcgtcgcgtg cttcgagttc
     5821 gacttcatgg gcggctcgat gggctcggtc gtgggcgagc gcttcgtgcg cggcgtccgg
     5881 gccgccgtcg agcagcgcgt gcccttcgtc tgctacacgg ccaccggcgg cgcgcgcatg
     5941 caggagggcc tgttctcgct gctgcagatg gccaagacca acggcgcgct gacgctgttg
     6001 tccaaggcga agctgcccta catcagcgtt ctgaccgacc cgaccatggg cggcgtgtcg
     6061 gcgagcttcg cgttcatcgg cgacgtcgtg atcgccgagc ccaaggcgct gatcggcttc
     6121 gccgggccgc gcgtcatcga gaacaccgtg cgcgagaagc tgcccgaggg cttccagcgc
     6181 tccgagttcc tgctgaagaa gggcgcgatc gacctgatct gcgatcgccg ccagctgcgc
     6241 ccgacgatcg cgcggctgct cgcgcagctg cagcgccaga gcgccgacgc cgtcgcctga
     6301 cgggccccca cggtcgctag cgctccctgc ccccccgagg gggcttgcgc gtgcggcccg
     6361 gcggagccgg atccgccgct tccccgcgtc tcgcttggga cgggcgagcg gcagcgccgg
     6421 aaccgccttt cgcttcgcgg acgtcgtgcc ctcgacgtcc gtttgttttt ggagtcgcca
     6481 tgacccatcc ccgcacgctg gccgagtggc tcgcgcactg cgagcgcctg catccgaaga
     6541 ccatcgacat gacgctgtcg cgcgtgcaca agatggtcga gcgcctgcag gtgcgtttcg
     6601 aggcgccggt gatctcggtg gccggcacca acggcaaggg ctcgacctgc gcgatgctgg
     6661 acgcgatcgc gcgcgcggcc ggctaccgcg tcggcctgtt cagcaagccg cacctggtgc
     6721 acttcgagga gcgctgccgc atcgacggcg cgatgatcac cgaggccgag ctgctgccgc
     6781 acttcgaggc ggtcgagcgg gcgcgcggcg acctgcagct gacctacttc gagttcacga
     6841 cgctcgccat cctgcacgcg ctggcggcca cgccgctgga tctcgtgatc ctcgaggtcg
     6901 gcctgggcgg ccggctggac gcggtcaacg gcatcgacgc cgacgtggcg gtcgtcacga
     6961 gcatcgacat cgaccacgtg gagtacctcg gccccgaccg cgaatcgatc ggccgcgaga
     7021 aggcgcacat cgcgcgtccg ggccggccgc tgatcgtggg cgacccgatg ccgccggcga
     7081 gcatccaggc ctacgcggaa tcgatcggcg ccgacctgtg gctgcagggg cgcgacttca
     7141 actactcggg cgatcgccag cagtggtcgt ggagcggccg cgggcggcgc tggaacgcgc
     7201 tcggctaccc ggcgctgcgc ggcgccaacc agctgctcaa cgcctcgggc gcgctcgcgg
     7261 cgctggaggc gctgcgcgag cgcctgcccg tgacggcgca ggctgtgcgc gtggggctcg
     7321 cctcggtgga gctgcccggg cgcttccaga tcgtcccggg gcagccggcg ctggtgctgg
     7381 acgtcgcgca caacccgcat tccgtcgcgg cgctggcgct gaacctggac cagatgggct
     7441 tcttcccgcg caccttcgcg gtgttcgggg cgatgcacga caaggatctc gccggcgccg
     7501 tcggcaagat cgtccacctg gtcgatgact ggcacgtcac cgacctgccg ctcgcgcggg
     7561 ccgccaaggc cgcggagctg gccgagacga tctgggcgct caccgcggcg cagccgcctt
     7621 ccgcgcccaa gccgcgcgtg acgacgcacc gttcgccgca ggaagcgctc gcggcggcga
     7681 tgtcgcaggc agacccggct gatagaatcg tggtcttcgg ctcgttctac accgtgggcg
     7741 gcgtgctgaa ggacggcctg ccgcgagggg tggcccgaca cgtgggttga tccggcggcg
     7801 ccgcgttccg atcgaacgcc gcgcgaggcg ccgcgcgacc cgtccggccc cgggcatcga
     7861 cgagaaggca tggggctgct ggctctcttc caacgcaaca agcagaccgc cgccaagccc
     7921 ggccgcgcgg gacgggcggg acgctcgccg gcgcgccagg cggcggccgc gagagatcgg
//
LOCUS       DASVLN010000901         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_532653, whole genome shotgun sequence.
ACCESSION   DASVLN010000901 DASVLN010000000
VERSION     DASVLN010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532653"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VF453_21170"
     CDS             complement(<1..578)
                     /locus_tag="VF453_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7690231.1"
                     /translation="MTEPTTPATPPPGANAAWRARSLRAVWHPCTQMKAHGTPGASTP
                     GDVPLVAIASAHGPWLVDVEGRRILDGISSWWTNLFGHGHPAIRAALVDQLERLDHVM
                     LAGLTHPPVVELSERLAALTGLGHAFYASDGASATEIALKMSAHHWRNAGRPAKHRFV
                     ALQGGYHGETAGALGVTDIALFRSAYAPLLHPA"
     gene            790..2052
                     /locus_tag="VF453_21175"
     CDS             790..2052
                     /locus_tag="VF453_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HEX7690232.1"
                     /translation="MDTRQKLIILADAAKYDASCASSGTAARDSSDGRGLGSTEGAGI
                     CHSYAPDGRCISLLKVLLTNWCVYDCLYCVNRESSNVARARFRVDEVVGLTLDFYRRN
                     VIEGLFLSSGIVRSPDYTMEQVIEVARRLREEHDFRGYIHLKTIPDASDELVLKAGRY
                     ADRLSVNIELPTADALQALAPEKESGAIRRSMARIKLRLDERADDAAPRVPRSMAAAA
                     PRPAAPPRFAPAGQSTQMIVGADASDDRTILRTSATLYGAYGLKRVYYSAFSPIPHAS
                     AALPPRAAPLMREHRLYQADWLMRFYGFAQDEICTEDGMLSLDVDPKLAWALANPAQF
                     PVDLNRAPRERLLRVPGLGVRSVDRVLAARRARSVRRADLDRLRVPVTKVLPFVLLPD
                     HVPREADGARLRRVVERRPAAAQGELFA"
     gene            2207..>3037
                     /locus_tag="VF453_21180"
     CDS             2207..>3037
                     /locus_tag="VF453_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HEX7690233.1"
                     /translation="MIAEVAAIAQDAPAAISLDGVTDWAGFSRAVRLLVARGVAPGGV
                     RWQVAGEDDVDLFGAAAMPARALPEPPPMNLPRAFVESAREVFLHADADRFALLHGLA
                     ARVAADARHWDDPLHPERLHFERCLREVRREIHKMHAFVRFRPIPTEGEGEDDGALRH
                     VAWFEPAHHVLEAAAPFFARRFATLRWAILTPRASVAWNGRALVHGPGARREDAPAAD
                     AGEALWLAYYRSIFNPARVKLAMMKREMPVRFWRNLPEARQVGPMLAEAADREHAMIA
                     A"
BASE COUNT          391 a         1123 c         1103 g          420 t
ORIGIN      
        1 gccggatgca gcaacggcgc gtaggccgag cggaacaggg cgatatcggt cacgcccagc
       61 gcgcccgcgg tctcgccgtg gtagcccccc tgcagcgcga cgaatcggtg cttcgccggc
      121 cggccggcgt tgcgccagtg gtgggcgctc atcttcagcg cgatctccgt ggccgaggcg
      181 ccgtcgctcg cgtagaacgc atggccgagg ccggtgagcg cggccaggcg ctcggacagc
      241 tcgacgaccg gcggatgcgt caggcccgcg agcatcacgt ggtcgagccg ctcgagctgg
      301 tcgaccagcg ccgcgcggat cgcgggatgg ccgtggccga acaggttcgt ccaccagctg
      361 ctgatgccgt ccaggatccg ccggccctcg acgtccacga gccacgggcc gtgcgcggac
      421 gcgatcgcga ccagcggcac gtcgccgggc gtcgatgcgc cgggcgtgcc gtgcgccttc
      481 atctgcgtgc agggatgcca gacggcgcgc aggctgcgcg cgcgccacgc ggcgttggcg
      541 cccgggggcg gcgtggccgg ggtcgtcggc tcggtcattc catctccttg gccgcgcgcg
      601 gcgtcgtcgg ggacgccggc gctccaggtc cggcggcgtg ccggacatcg cgaaaaaaag
      661 gatgcgactt taagccatgg ccgcgcacgg ccgggctgtg caaatcgaca ggggcgtgca
      721 gtctttccag cctgcttcgg ccgcgatggc gtagactgta ttttcgtaca gcaatatccg
      781 tgtgccaccg tggacacccg ccagaagctc atcatccttg ccgacgcggc caagtacgac
      841 gcctcgtgcg cgtccagcgg cacggcggcg cgcgactcca gcgacggccg cggcctggga
      901 tcgacggagg gcgcgggcat ctgccacagc tacgcgccgg acggacgctg catctcgctg
      961 ctgaaggtgc tgctgaccaa ctggtgcgtg tacgactgcc tgtactgcgt caaccgcgag
     1021 tcgagcaacg tcgcgcgcgc gcgcttccgc gtcgacgagg tggtcgggtt gacgctggac
     1081 ttctaccgcc gcaacgtcat cgaggggctg ttcctgagct cgggcatcgt gcgctcgccc
     1141 gactacacga tggagcaggt gatcgaggtc gcgcgccgcc tgcgcgagga gcacgacttc
     1201 cgcggctaca tccacctgaa gacgatcccc gacgcgagcg acgagctggt gctcaaggcc
     1261 ggccgctacg ccgaccgcct gagcgtcaac atcgagctgc cgacggccga cgcgctgcag
     1321 gcgctcgcgc ccgagaagga gagcggcgcg atccggcgct cgatggcgcg catcaagctg
     1381 cggctggacg agcgtgccga cgacgccgcg ccgcgcgtcc cgcgttccat ggccgcggcg
     1441 gcgccccggc ccgcggcgcc gccgcgcttc gcgccggcgg ggcagagcac gcagatgatc
     1501 gtcggcgccg acgcctcgga cgaccgcacg atcctgcgca ccagcgcgac gctgtacggt
     1561 gcctatggcc tgaagcgcgt ctactactcg gcgttcagcc cgatcccgca tgcgtccgcc
     1621 gcgctgccgc ctcgcgccgc gccgctcatg cgcgagcacc ggctctacca ggccgactgg
     1681 ctcatgcgct tctacggctt cgcccaggac gagatctgca ccgaggacgg catgctgtcg
     1741 ctggacgtcg accccaagct ggcctgggcg ctcgcgaacc cggcgcagtt cccggtcgac
     1801 ctcaaccgcg cgccgcgcga gcgcctgctg cgcgtgccgg ggctgggcgt ccgctcggtc
     1861 gatcgcgtcc tggccgcacg ccgcgcgcgc agcgtgcgcc gggccgacct cgaccgcctg
     1921 cgcgtacccg tgaccaaggt actgcccttc gtgctgctgc ccgaccacgt gccgcgcgag
     1981 gccgacggcg cgcggctgcg gcgggtcgtc gagcgtcgcc ccgcggcagc gcagggggag
     2041 ttgtttgcat gagccctgcc cggccgcccg aaagggctcg caccgcaggc cgcgaggccg
     2101 gaggtttcct gctgagccct gcccggccgc ccgaaagggc tcgcaccgca ggccgcgagg
     2161 ccggagcctg cctgctgcgc cctgcacggc gggcggagct cgtgtcgtga tcgccgaagt
     2221 cgccgcgatc gcgcaggacg cgccggccgc gatctcactc gatggcgtga ccgactgggc
     2281 cggcttctcg cgcgcggtgc gcctgctcgt cgcccgcggc gtcgcgcccg gcggggtgcg
     2341 ctggcaggtc gcgggcgagg acgacgtcga ccttttcggc gccgccgcga tgcccgcgcg
     2401 tgcgttgccc gagccgccgc cgatgaacct gccacgcgcc ttcgtcgagt cggcgcgcga
     2461 ggtgttcctg cacgccgacg ccgaccgctt cgcgctgctg cacggtctgg ccgcccgcgt
     2521 cgcggccgat gcgcggcatt gggacgaccc gctccatccc gaacgcctgc acttcgagcg
     2581 ctgcctgcgc gaggtgcggc gcgagatcca caagatgcat gcgttcgttc gctttcgccc
     2641 gatcccgacc gaaggcgagg gcgaggacga cggggcgctg cggcatgtcg cctggttcga
     2701 gccggcgcac cacgtgctcg aagccgccgc gccgttcttc gcccgccgct tcgcgacgct
     2761 gcgctgggcg atcctcacgc cgcgggcgag cgtcgcatgg aacggccgcg cgctggtgca
     2821 cggtccgggc gcccgccgcg aggacgcccc cgccgccgac gccggcgagg cgctctggct
     2881 cgcgtactac cgcagcatct tcaacccggc gcgcgtgaag ctggcgatga tgaagcgcga
     2941 gatgccggtg cgcttctggc gcaacctgcc cgaggcgcgg caggtcggcc ccatgctggc
     3001 cgaggcggcg gatcgcgagc acgcgatgat cgcggcc
//
LOCUS       DASVLN010000902         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_369782, whole genome shotgun sequence.
ACCESSION   DASVLN010000902 DASVLN010000000
VERSION     DASVLN010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369782"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VF453_21185"
     CDS             <1..302
                     /locus_tag="VF453_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX7690234.1"
                     /translation="ESAAILVANDTGLSHVAAATGTRSVIVASGSDVARWRPLDPARH
                     VVLWHAAPCRPCAHPVCPTAHECAAGVPAAAVIDAAIQLLGDSVPCPTPTAACAS"
     gene            269..1237
                     /locus_tag="VF453_21190"
     CDS             269..1237
                     /locus_tag="VF453_21190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7690235.1"
                     /translation="MPDSNGRLRILTWHVHGNYLWYLTQVPHEFYLVTDRDRTTHHSG
                     RSGTLPWGDNVHEAPVDQIRDMEFDVILWQSRAEYENEQTTLLSAAQQRLPMVYLEHD
                     PPLEHPTDQKHWADDEKVTVVHVTGFNALMWDHRDSPVEVIEHGVRLLFDARYDGRHG
                     KGVTVANNIATRGRRLGADVFVRLREQVPLTLVGMGSMEFGGHGEVANRELPAFISHY
                     RFFLNPIRYTSLGLSVIEAMMVGLPIVGLATTEMSTVIVNGVNGWLETRPEKLVEPMR
                     ALIDDPGLARRWGEAARRTAEERFGIARFVADWDRVLRHAAGASIH"
     gene            1258..2304
                     /locus_tag="VF453_21195"
     CDS             1258..2304
                     /locus_tag="VF453_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX7690236.1"
                     /translation="MTLPLHERRIAVTGGAGFIGSHLCERLLARGDEVLCIDNFYTGS
                     REFIAPLLTHPRFELVRHDVTFPLYIECDAIFNLACPASPIHYQMDPVQTTKTSVHGA
                     INLLGLAKRLRAPILQASTSEVYGDPTCHPQVEGYWGHVNPIGPRACYDEGKRCAETL
                     FMDYHRQHRVPVKIARIFNTYGPHMHPGDGRVVSNFIVQALTGEPITLYGDGLQTRSL
                     CYVDDTVAGLMRLMDSALSVTGPVNIGNPVELSMRDIAHTILDLTGSRSTIVHRPLPA
                     DDPRQRCPDISQARELLDWRPTVGVREGLSRTIAWFDELLRRRGAIVAEHAYDAVPEP
                     VPVPAPEPPRTLTT"
BASE COUNT          367 a          944 c          792 g          341 t
ORIGIN      
        1 tcgagtcggc ggccatcctg gtcgccaacg acaccggcct gtcgcacgtc gccgcggcga
       61 caggcacgcg cagcgtgatc gtcgccagcg gctccgacgt cgcccgctgg cggccgctcg
      121 accctgcgcg ccacgtcgtc ctgtggcacg cggcgccgtg ccgcccgtgc gcccatcccg
      181 tctgcccgac cgcgcacgaa tgcgcggccg gcgttcccgc cgccgccgtg atcgatgcgg
      241 cgatccaact gctcggagat tccgtcccat gcccgactcc aacggccgcc tgcgcatcct
      301 gacctggcac gtccacggca actacctctg gtacctcacc caggtacccc acgagttcta
      361 cctcgtcacc gaccgcgacc gcacgacgca ccacagcggc cgcagcggga cgctgccctg
      421 gggcgacaac gtgcacgagg cgccggtcga ccagatccgc gacatggagt tcgacgtcat
      481 cctctggcag tcgcgcgccg agtacgagaa cgagcagacc acgctgctgt ccgcggcgca
      541 gcagcggctg ccgatggtct acctcgagca cgacccgccg ctcgagcacc cgaccgacca
      601 gaagcactgg gccgacgacg agaaggtcac cgtggtgcac gtcaccggct tcaacgcgct
      661 gatgtgggat caccgcgact cgccggtcga ggtcatcgag cacggcgtcc ggctgctctt
      721 cgacgcgcgc tacgacggcc gccacggcaa gggcgtcacc gtcgcgaaca acatcgccac
      781 gcgcgggcgg cgcctcggcg cggacgtgtt cgtgcggctg cgcgagcagg tgccgctcac
      841 gctcgtgggc atgggctcga tggaattcgg cgggcacggc gaggtcgcca accgcgagct
      901 gccggccttc atctcgcact accgcttctt cctcaacccg atccgctaca cgagcctcgg
      961 gctgtcggtg atcgaggcca tgatggtcgg cctgcccatc gtgggcctcg cgacgaccga
     1021 gatgagcacg gtcatcgtca acggcgtcaa cggctggctc gagacgcggc ccgagaagct
     1081 cgtcgagccc atgcgcgcgc tgatcgacga ccccgggctc gcccgccgct ggggcgaggc
     1141 cgcgcgccgc accgccgagg agcgcttcgg catcgcccgc ttcgtcgccg actgggaccg
     1201 cgtgctgcgc cacgcggccg gcgcgagcat ccactgagac agcgcaagga ccctgccatg
     1261 acgcttcccc tgcacgaacg ccgcatcgcc gtcaccggcg gtgccggctt catcggatcg
     1321 cacctgtgcg agcgcctgct ggcgcgcggc gacgaggtgc tgtgcatcga caatttctat
     1381 accggctcgc gcgagttcat cgcgccgctg ctgacgcatc cgcgcttcga gctcgtgcgc
     1441 cacgacgtca ccttcccgct ctacatcgag tgcgacgcga tcttcaacct cgcgtgcccg
     1501 gcctcgccga tccactacca gatggatccg gtgcagacga ccaagaccag cgtgcacggc
     1561 gcgatcaacc tgctgggcct ggccaagcgg ctgcgcgcgc cgatcctgca ggcgtcgacg
     1621 agcgaggtct acggcgaccc gacgtgccac ccccaggtcg agggctactg gggccacgtg
     1681 aatcccatcg gcccgcgcgc ctgctacgac gagggcaagc gctgcgccga gacgctcttc
     1741 atggactacc accgccagca ccgcgtgccg gtgaagatcg cgcggatctt caacacctac
     1801 ggcccgcaca tgcacccggg cgacgggcgc gtcgtctcca acttcatcgt gcaggcgctc
     1861 accggcgagc cgatcaccct ctacggcgac ggcctgcaga cgcgctcgct gtgctacgtc
     1921 gacgacaccg tggccggcct gatgaggctg atggactccg cgctgagcgt cacgggcccg
     1981 gtcaacatcg gcaacccggt cgagctcagc atgcgggaca tcgcgcacac catcctcgac
     2041 ctgaccggtt cgcgctcgac catcgtgcac cgcccgctgc ccgcggacga cccgcgccag
     2101 cgctgcccgg acatctcgca ggcacgcgag ctgctcgatt ggcggccgac cgtcggcgtg
     2161 cgcgaggggc tgtctcgcac catcgcgtgg ttcgacgagc tgctgcgccg gcgcggcgcg
     2221 atcgtcgccg agcacgccta cgacgccgtg cccgaacccg tcccggtgcc cgcgcccgag
     2281 cctccgcgaa cgctgaccac ctgaccccac gatgctgcag ccgcaggcca cgatcgccga
     2341 ccacgacctc gcgcgcgcgc gccgcgcgct ggtcggcgac tcgggctggg cgagcgtctg
     2401 cggcgcgctg ttcggcggca tcgtgctggc gggctacgcg atcg
//
LOCUS       DASVLN010000903         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_45451, whole genome shotgun sequence.
ACCESSION   DASVLN010000903 DASVLN010000000
VERSION     DASVLN010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45451"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /gene="ugpC"
                     /locus_tag="VF453_21200"
     CDS             complement(<1..1029)
                     /gene="ugpC"
                     /locus_tag="VF453_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX7690237.1"
                     /translation="MANLQLTQVRKAFGDIRILSDIDLEIRDGEFMVFVGPSGSGKST
                     LLRTIAGLEDISGGTLRIGGRVVNDVPPAERGIAMVFQSYALYPHMSLFDNMAFGLQI
                     AKVPKDEIQRLVHQAAKILNIEHLLDRRPKALSGGQRQRVAIGRAIVRKPEVFLFDEP
                     LSNLDAALRVRMRYEFAKLHEELKTTMIYVTHDQVEAMTLADRIVVLAGGRIEQVGTP
                     LELYEHPDNLFVAGFIGSPKMNFVAAEVVSANAAEAVVRLSTGALVRCEVDAARAKPG
                     DKVTLGVRPEHLESRAAAAAGEANQLAATVTFVEQLGGTTHAYCDYPGASETLVCELR
                     GSTRLRAGA"
BASE COUNT          228 a          524 c          509 g          245 t
ORIGIN      
        1 cgcgcccgcg cgcaggcgcg tcgagccgcg cagctcgcac acgagcgtct cggaggcgcc
       61 cgggtagtcg cagtaggcgt gcgtggtgcc gcccaattgc tcgacgaagg tgacggtggc
      121 cgcgagctgg ttggcctcgc ccgcggccgc ggcggcgcgc gactcgaggt gctccggtcg
      181 cacgcccagc gtcaccttgt cgcccggctt cgcgcgcgcg gcgtcgacct cgcagcgcac
      241 gagcgcgccg gtcgacagcc gcacgaccgc ctcggccgcg ttcgcggaga cgacctcggc
      301 cgcgacgaag ttcatcttcg gcgagccgat gaagccggcg acgaagaggt tgtcgggatg
      361 ctcgtagagc tccagcggcg tgccgacctg ctcgatgcgg ccgcccgcga gcacgacgat
      421 gcggtcggcc agcgtcatcg cctcgacctg gtcgtgggtg acgtagatca tcgtcgtctt
      481 cagctcctcg tggagcttgg cgaactcgta gcgcatgcgc acgcgcagcg cggcgtcgag
      541 gttggacagc ggctcgtcga acaggaagac ttccggcttg cgcacgatcg cgcggccgat
      601 cgccacgcgc tggcgctggc cgcccgacag cgccttcggc cggcgatcca gcaggtgctc
      661 gatgttgagg atcttcgcgg cctggtgcac gaggcgctgg atctcgtcct tgggcacctt
      721 ggcgatctgc agcccgaacg ccatgttgtc gaacaggctc atgtgcgggt agagcgcgta
      781 gctctggaac accatcgcga tgccgcgctc ggcgggcggg acgtcgttga ccacgcgccc
      841 cccgatgcgc agcgtgccgc cgctgatgtc ctcgaggccc gcgatcgtgc gcagcagcgt
      901 cgacttgccg ctgcccgagg ggcccacgaa caccatgaac tcgccgtcgc ggatctcgag
      961 gtcgatgtcg ctcaggatgc ggatgtcccc gaacgccttg cgcacctgcg tcagctggag
     1021 gtttgccact gtcgtctgtc tcctgtggcg gtgcgctcgt ctccgcggcg cccgcgctgg
     1081 tcttcgcgat ccgctctcgc ggtcgtcaat gtagagaata tacaaagtca gagaccgtcg
     1141 cgcaatgcgt aactacttaa attacagcgc catgctcact gctacgacta caggaaatgc
     1201 aattgtcagg gataaccctt tgtaggcgaa ctacacaaaa acttctcaac aggcgatact
     1261 ggcatcattc tgccgcaccg gcgcagccca gggctccgcc gcgtccggcc tcgccgacaa
     1321 ggattcctga atgagcaagt cttccgtcgc cgccccggcg cccgactccc agaagccgcg
     1381 cgcgggcgaa ggcgacgccc gtccgccgga gggtgcgttg aacgccgagt tcgccgccgc
     1441 gtggcggcgc gagctcgcgc agcgcagcga cgcgtcctcg gccgcggcat gcctgcgtgc
     1501 cgcggc
//
LOCUS       DASVLN010000904         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_478195, whole genome shotgun sequence.
ACCESSION   DASVLN010000904 DASVLN010000000
VERSION     DASVLN010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478195"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="VF453_21205"
     CDS             <1..1454
                     /locus_tag="VF453_21205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7690238.1"
                     /translation="TADIEAALADVRPSAPPRALALAQARVRAMLDELAALRREVRQR
                     QSMSVMFLEEVEDVRGQVMLAFALVVALLVAVVGLGVRWIVVARRERAIRERAEQLAV
                     LAEAERARAETVGRDKARFLGMLSHELLTPLQSILSTMDVIESRGRVDAREPAFTRLR
                     ESTRSLRGRISDLVDFAKMSSGRLETRIRGFQVDALVDTALRDLEETLAARNLDVHWE
                     AGPELAQRIYSDPARLRQILDNLLTNAIKYTERGGIAIHVRIAEQGDLLRLEVADTGV
                     GIPPEEIPHLFEPFYRSPKTAAMAEGSGLGLAVVRSLVDLMGGSIRVDSTPGRGTSIA
                     LEVPIALGADAEVEPPMRIDIRRPVLVVDDARDARHAIAEVIRALGIDAHEVDCGEQA
                     LVQAQLHDYQAILLDMQMPGLSGAEVARRLRRPGGRHEKTFLVLVSAYSELDEASAAA
                     LFDARIDKPASRRDLASALSRAAAAASRAPPSG"
BASE COUNT          227 a          547 c          584 g          230 t
ORIGIN      
        1 ccaccgccga catcgaggcg gcgctcgcgg acgtgcggcc gagcgctccc ccgcgcgcgc
       61 ttgcgctcgc gcaggcgcgg gtgcgggcga tgctggacga actggccgcg ctgcgccgcg
      121 aggtgcgcca gcgccagtcg atgtccgtga tgttcctcga ggaggtcgag gacgtgcgcg
      181 gccaggtgat gctcgccttc gcgctggtcg tcgcgttgct ggtggccgtc gtggggctcg
      241 gcgtacgctg gatcgtggtg gcgcggcggg agcgcgcgat ccgcgagcgc gccgagcagc
      301 tggccgtcct cgcggaggcc gagcgcgcgc gggccgagac cgtcgggcgc gacaaggcac
      361 gtttcctggg catgctgagc cacgagctgc tgacgccgct gcagtccatc ctgagcacga
      421 tggacgtgat cgagtcgcgc ggccgggtcg acgcgcgcga gccggcgttc acccgcctgc
      481 gcgaaagcac gcggtcgctg cgcgggcgca tcagcgacct ggtcgacttc gccaagatgt
      541 cctcggggcg cctggagacc cgcatccgcg gcttccaggt cgacgcgctc gtcgacacgg
      601 cgcttcgtga cctcgaggag acgcttgccg cacgcaacct cgacgtccac tgggaggccg
      661 ggccggagct cgcgcaacgc atctactcgg atccggcgcg gctgcgccag atcctcgaca
      721 acctgctcac caacgcgatc aagtacaccg agcgcggcgg catcgcgatc cacgtgcgga
      781 tcgcggagca gggcgacctg ctgcggctgg aggtcgcaga cacgggcgtg ggcatcccgc
      841 ccgaagagat cccgcacctg ttcgagccgt tctaccgttc gcccaagacg gcggcgatgg
      901 ccgagggcag cgggctcggg ctcgcggtgg tgcgcagcct cgtcgacctg atgggcggca
      961 gtatccgcgt cgactccacg ccgggccgcg gcacctcgat cgcgctggag gtgccgatcg
     1021 cgctcggcgc cgatgcggag gtcgagccgc cgatgcgcat cgacatccgt cggccggtgc
     1081 tggtcgtcga cgacgcgcgc gacgcgcggc atgcgatcgc cgaggtgatc cgggcccttg
     1141 gcatcgatgc ccacgaagtc gactgcggcg agcaggcgct cgtgcaggcg cagttgcacg
     1201 actaccaggc gatcctgctg gacatgcaga tgccggggct gtccggcgcg gaggtcgccc
     1261 ggcggctgcg ccggccgggc ggtcgccatg agaagacctt cctggtgctg gtgtccgcct
     1321 acagcgaact cgacgaggcc agcgcggcgg cgctgttcga tgcgcgcatc gacaagccgg
     1381 ccagccgccg cgacctggcg tcagcgctgt cgcgggccgc cgctgcggcc agccgcgcgc
     1441 cgccttcggg ctagctgtga aggttcaata ggttgttgcg ggtgttcacc cggagaggtt
     1501 ttgagagact gggaatcgcc aaaccccccg tcaacttcaa agccggaacc ggatgaacac
     1561 ccataagcat gcccgattga cgtacctc
//
LOCUS       DASVLN010000905         4497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_532876, whole genome shotgun sequence.
ACCESSION   DASVLN010000905 DASVLN010000000
VERSION     DASVLN010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532876"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VF453_21210"
     CDS             <1..141
                     /locus_tag="VF453_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7690239.1"
                     /translation="EMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKI
                     IE"
     gene            237..548
                     /gene="rpsJ"
                     /locus_tag="VF453_21215"
     CDS             237..548
                     /gene="rpsJ"
                     /locus_tag="VF453_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX7690240.1"
                     /translation="MQKQKIRIRLKAFDYKLIDQSALEIVETAKRTGAIVKGPVPLPT
                     RLQRFDILRSPHVNKTSRDQFEIRTHQRLMDIVDPTDKTVDALMKLDLPAGVDVEIKL
                     Q"
     gene            724..1395
                     /gene="rplC"
                     /locus_tag="VF453_21220"
     CDS             724..1395
                     /gene="rplC"
                     /locus_tag="VF453_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX7690241.1"
                     /translation="MSLSNRMGLLGRKVGMMRIFTDDGDAVPVTVIDVSNNRVTQVKT
                     ADTDGYAGVQVAFGTRKASRVSKPEAGHLAKAGVEAGEILKEFRVTAEIASEYKPGAT
                     VPVALFEAGQLVDVQGTSIGKGFAGTIKRHNFGSQRASHGNSRSHNVPGSIGMAQDPG
                     RVFPGKRMSGHLGDETVTVQNLDVVRVDAERSLLLVRGAVPGAKNGHVVVRHAVKVKA
                     KKGAN"
     gene            1395..2015
                     /gene="rplD"
                     /locus_tag="VF453_21225"
     CDS             1395..2015
                     /gene="rplD"
                     /locus_tag="VF453_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX7690242.1"
                     /translation="MQLELLNEQGQATSKVDAPDTVFARDYNEALVHQVVVAFQANAR
                     AGTRAQKNRETVKHSTKKPWRQKGTGRARAGMTSSPIWRGGGRAFPNSPDENFTQKVN
                     KKMYRAGMAAILSQLVRDGRLAVVESIDVDAPKTKLLAQKFKDMGLSSVMVISDNIGE
                     NLELASRNLPNVLVTEPRYADPLSLVFYKKVLVTKAAIEQLKEMFA"
     gene            2012..2332
                     /gene="rplW"
                     /locus_tag="VF453_21230"
     CDS             2012..2332
                     /gene="rplW"
                     /locus_tag="VF453_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX7690243.1"
                     /translation="MSATKKFDENRLATILMAPIISEKATNVAEKNNQVVFKVLRDAT
                     KPEIKAAVELLFPGKEVTAVSTVVQKGKVKRFGRNIGRRDHVKKAYVSLKEGQELNFS
                     GEAA"
     gene            2333..3157
                     /gene="rplB"
                     /locus_tag="VF453_21235"
     CDS             2333..3157
                     /gene="rplB"
                     /locus_tag="VF453_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX7690244.1"
                     /translation="MAVIKVKPTSPGRRAVVKVVHAHLHKGKPEASLLEPQKQKAGRN
                     NNGHITMRHKGGGHKHHYRVVDFVRNKDGIPAKVERIEYDPNRTAHIALLCYADGERR
                     YIIAPRGLEVGATVQSGAEAPIKAGNTLPIRNIPVGSIIHCVEMLPGKGAQIARSAGT
                     SVTLMAREGIYAQVRLRSGEVRKIHIDCRATIGETSNEEHNLRHYGKAGAIRWKGIRP
                     TVRGVAMNPVDHPHGGGEGRTGEGQVPVSPWNTMTKGYRTRNNKRTQTMIVSRRKK"
     gene            3171..3446
                     /gene="rpsS"
                     /locus_tag="VF453_21240"
     CDS             3171..3446
                     /gene="rpsS"
                     /locus_tag="VF453_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX7690245.1"
                     /translation="MARSLKKGPFVDHHLLAKAEKANANKDKKPIKTWSRRSTILPDF
                     IGLTIAVHNGKQHVPVYVSDQMVGHKLGEFALTRTFKGHPADKKAKR"
     gene            3459..3788
                     /gene="rplV"
                     /locus_tag="VF453_21245"
     CDS             3459..3788
                     /gene="rplV"
                     /locus_tag="VF453_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX7690246.1"
                     /translation="METKAIVRGVRLSADKGRLVADLIRGKKVGLALNTLEFTQKKAA
                     GIIKKALESAIANAEHNDGADIDDLKVTSIYVEQGTTLKRFSARAKGRGNRISKGTCH
                     IYVTVGN"
     gene            3804..>4497
                     /gene="rpsC"
                     /locus_tag="VF453_21250"
     CDS             3804..>4497
                     /gene="rpsC"
                     /locus_tag="VF453_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX7690247.1"
                     /translation="MGQKIHPTGFRLPVTRAWASRWYASNRNFSQMLAEDLKVREFLK
                     AKLKNAAVSRILIERPAKNARITIYSARPGVVIGKKGEDIENLKAELARRLGVPVAVN
                     IEEVRKPEIDAQLIADSITQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSSGRLNGI
                     EIARCEWYREGRVPLHTLKADIDYGFSEAKTTYGVIGCKVWVYRGDRLANGEPPVIPK
                     MEGEDDRRGPRRG"
BASE COUNT          925 a         1527 c         1420 g          625 t
ORIGIN      
        1 gagatggtga tgccgggcga caacgtgtcg atcacggtca agctgatcgc cccgatcgcg
       61 atggaagaag gcctgcgctt cgccatccgt gaaggcggcc gcaccgtcgg cgccggcgtc
      121 gtcgccaaga tcatcgagta actcttgcgt gatgcggacg cgcggccccc gcggcggcgc
      181 gtcctctccc acatcccgcc gggcgcgacc cggcaccgct ctttgaggaa cacatcatgc
      241 aaaagcaaaa gatccgcatc cgcctgaagg cgttcgacta caagctgatc gaccagtcgg
      301 cgctcgagat cgtcgagacc gccaagcgca ccggcgcgat cgtcaagggc ccggttcccc
      361 tgccgacccg cctgcagcgc ttcgacatcc tgcgctcgcc gcacgtcaac aagacgagcc
      421 gggaccagtt cgagatccgc acgcaccagc gcctgatgga catcgtcgac ccgacggaca
      481 agaccgtcga cgcgctgatg aagctggacc tgccggccgg cgtcgacgtc gagatcaagc
      541 tgcagtaatc gcggcggttg cgggttcgcc cgcatctgct acaatcgcag gctctgcgta
      601 aaaaagcccc tcggccatcg gtcgtggggc tttgcacgtt gaaccaagtc agcccggcca
      661 atcgctgtcg ggtatgtgaa acaaaggccc ggaagccgtc aggcggcggg ctggagaaaa
      721 accatgagtc tgagcaatcg catgggattg ctgggtcgca aggtgggcat gatgcgcatc
      781 ttcacggacg acggcgacgc cgtgcccgtg acggtgatcg acgtgtccaa caaccgcgtc
      841 acccaagtca agaccgccga caccgacggc tacgccggcg tccaggtcgc cttcggcacg
      901 cgcaaggcgt cgcgcgtctc caagccggaa gccggccacc tcgccaaggc gggtgtggag
      961 gcgggcgaga tcctgaagga attccgcgtc accgcggaga tcgcctccga atacaagccg
     1021 ggcgccaccg tgcccgtggc cctgttcgag gccggccagc tcgtcgacgt gcaaggcacc
     1081 tcgatcggca agggcttcgc gggcaccatc aagcgccaca acttcggctc gcagcgcgcg
     1141 tcgcacggta acagccgttc gcacaacgtg ccgggctcga tcggcatggc gcaggatccg
     1201 ggtcgcgtgt tcccgggcaa gcgcatgtcg ggtcacctcg gcgacgagac cgtcaccgtg
     1261 cagaacctcg acgtcgtccg cgtcgatgcc gagcgctcgc tgctgctcgt gcgcggcgcc
     1321 gtccccggcg cgaagaacgg ccacgtggtc gtccgtcacg ccgtcaaggt caaggccaag
     1381 aagggagcca actgatgcaa ctcgagctcc tcaacgaaca aggccaggcc acttcgaagg
     1441 tcgacgcgcc ggacaccgtg ttcgcgcgtg actacaacga agccctggtg caccaggtcg
     1501 tcgtcgcgtt ccaggccaac gcccgcgccg gcacccgtgc gcagaagaac cgtgagaccg
     1561 tcaagcattc gacgaagaag ccgtggcgcc agaagggcac cggccgcgct cgcgccggca
     1621 tgacctcgtc gccgatctgg cgtggaggcg gtcgcgcctt cccgaacagc ccggacgaga
     1681 acttcacgca gaaggtcaac aagaagatgt atcgcgccgg catggccgcg atcctctcgc
     1741 agctcgtgcg cgacggccgc ctggcggtgg tcgagtcgat cgacgtcgac gcgccgaaga
     1801 ccaagctgct ggcccagaag ttcaaggaca tgggcctcag ctccgtcatg gtcatctccg
     1861 acaacatcgg cgagaacctc gagctggctt cgcgcaacct gcccaacgtg ctcgtgaccg
     1921 agccgcgtta tgccgatccc ctgtcgctgg tcttctacaa gaaggtgctg gtgaccaagg
     1981 ccgcgatcga gcagctcaag gagatgttcg catgagcgcc accaagaaat tcgacgaaaa
     2041 ccgtctcgcg acgatcctga tggccccgat catctcggag aaggccacga acgtcgccga
     2101 gaagaacaac caagtggtgt tcaaggtcct gcgcgacgcg accaagcccg agatcaaggc
     2161 ggctgtcgag ctgctgttcc cgggcaagga agtgaccgcc gtctcgaccg tcgtccagaa
     2221 gggcaaggtc aagcgcttcg gtcgcaacat cggccgccgc gaccacgtca agaaggcgta
     2281 cgtgtcgctg aaggaaggcc aagagctcaa cttctccggg gaggctgcgt aaatggccgt
     2341 catcaaagtc aagccgactt cgcctggccg ccgcgcggtc gtgaaggtcg tgcacgccca
     2401 cctgcacaag ggcaagcccg aggcgtcgct gctcgagccc cagaagcaga aggccggccg
     2461 caacaacaac ggccacatca cgatgcgcca caagggcggt ggtcacaagc accactaccg
     2521 cgtggtcgat ttcgtgcgca acaaggacgg catcccggcg aaggtcgagc gcatcgagta
     2581 cgacccgaac cgcaccgctc acatcgccct gctgtgctac gccgacggcg agcgccgcta
     2641 catcatcgcc ccgcgcggcc tggaagtcgg cgcgaccgtc cagtccggcg ccgaggcgcc
     2701 gatcaaggcc ggcaacacgc tcccgatccg caacatcccg gtcggctcca tcatccactg
     2761 cgtggagatg ctgccgggca agggtgccca gatcgcccgc tcggccggca cgtcggtgac
     2821 gctgatggcg cgcgaaggca tctacgccca ggttcgcctg cgctcgggtg aagtgcgcaa
     2881 gatccacatc gactgccgcg ccaccatcgg cgagacctcc aacgaagagc acaacctgcg
     2941 ccattacggc aaggccggtg cgatccgctg gaagggcatc cgcccgacgg tccgcggtgt
     3001 cgcgatgaac ccggtcgacc acccgcacgg cggcggcgaa ggccgcacgg gcgaaggcca
     3061 ggtcccggtg tcgccgtgga acacgatgac caagggctat cgcacgcgca acaacaagcg
     3121 cacgcagacg atgatcgtct cgcgccgcaa gaagtaaggg ctgaacgaac atggcacgtt
     3181 ctctcaagaa gggtccgttc gtggaccacc acctgctcgc caaggccgag aaggccaatg
     3241 cgaacaagga caagaagccc atcaagacct ggtcgcgtcg ttcgacgatc ctcccggact
     3301 tcatcggcct gacgatcgcc gtccacaacg gcaagcagca cgttcccgtg tacgtgagcg
     3361 accagatggt cggccacaag ctgggtgaat tcgcgctgac gcggaccttc aagggtcacc
     3421 cggccgacaa gaaggccaag cgctaaggag cccacgacat ggaaaccaaa gcaatcgtcc
     3481 gtggcgtgcg cctgtccgcc gacaagggcc gcctggtcgc cgacctgatc cgtggcaaga
     3541 aggtgggcct cgcgctcaac accctcgagt tcacgcagaa gaaggccgcc ggcatcatca
     3601 agaaggcgct cgagtcggcc atcgccaacg cggagcacaa cgacggcgcg gacatcgacg
     3661 acctgaaggt cacgtcgatc tacgtcgagc agggcaccac gctgaagcgt ttctcggccc
     3721 gcgcgaaggg tcgtggcaac cgcatcagca agggtacctg ccacatctac gtgaccgtcg
     3781 gcaactgacg cgcaaggaag atcatgggac agaaaatcca cccgaccggc ttccgcctgc
     3841 ccgtcacccg ggcgtgggcg tcgcgctggt acgcctcgaa ccgcaacttc agccagatgc
     3901 tggccgagga cctgaaggtt cgcgagttcc tgaaggccaa gctcaagaat gccgcggtct
     3961 cgcgcatcct gatcgagcgc cccgccaaga acgcccgcat cacgatctac tcggcgcgtc
     4021 cgggcgtcgt gatcggcaag aagggcgagg acatcgagaa cctgaaggcc gagctggcgc
     4081 gccgcctggg cgtgcccgtc gccgtcaaca tcgaggaagt gcgcaagccc gaaatcgatg
     4141 cgcagctgat cgccgactcg atcacccagc agctcgagaa gcgcatcatg ttccgccgcg
     4201 ccatgaagcg cgcgatgcag aacgcgatgc gcctcggcgc ccagggcatc aagatcatgt
     4261 cgtcgggtcg cctgaacggc atcgagatcg cccgctgcga gtggtatcgc gaaggccgag
     4321 tgcccctcca cacgctgaag gccgacatcg actacggctt ctcggaagcc aagacgacct
     4381 acggcgtcat cggctgcaag gtgtgggtct accgcggtga ccgcctcgcc aacggcgagc
     4441 cgccggtcat cccgaagatg gaaggcgaag acgaccgccg cggcccgcgt cgcggcc
//
LOCUS       DASVLN010000906         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_101943, whole genome shotgun sequence.
ACCESSION   DASVLN010000906 DASVLN010000000
VERSION     DASVLN010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101943"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..674
                     /locus_tag="VF453_21255"
     CDS             228..674
                     /locus_tag="VF453_21255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7690248.1"
                     /translation="MKTVQPDSFLTLHYRLCGPDGAEVVSTFDDKPATLSLGAGQLAP
                     AIEACLVGMEEGARMTWHLEAGAAYGQRNPEMLQRVKRSLLAQHGDPDEDYRVGDVVE
                     FPTPDGHGRFAGVVREAGDDWLLFDFNHPLAGQPVTFEAQLIGVMN"
     gene            790..1794
                     /gene="ispH"
                     /locus_tag="VF453_21260"
                     /gene_synonym="lytB"
     CDS             790..1794
                     /gene="ispH"
                     /locus_tag="VF453_21260"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7690249.1"
                     /translation="MSAELEAKVVGEVLLAQPRGFCAGVDRAIEIVERALEKFGRPIY
                     VRHEIVHNTFVVNDLKSKGAIFIEDLAEVPPGATLVFSAHGVSKAVRDEADARGFSVF
                     DATCPLVTKVHVEVSKLAREGYEFIMIGHKGHPEVEGTMGQLTEGIYLVEDVPDVERV
                     HPRDPSKLAVVTQTTLSVDDAAEILAAVKRRFPLIREPKKQDICYATQNRQDAVKFIA
                     PKVDIVIVVGSPTSSNSNRLRELAERLGTPAYMVDAASDLQPAWFEGRSRVGLTAGAS
                     APDVLVQQVIDRLKALGAISVRNVEGVEETVEFPLPLGLGDRSMNELRAARAGEAPRS
                     "
     gene            1921..3216
                     /gene="serS"
                     /locus_tag="VF453_21265"
     CDS             1921..3216
                     /gene="serS"
                     /locus_tag="VF453_21265"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7690250.1"
                     /translation="MLDINLLRRDLPAVVAGLEKRKNPQPYLDVAAYTALEAERKAVQ
                     VRSEEGQARVNALSRQIGQAKGKGEDASALMAEVAELKAQKAAGDLRLEELQAELSTM
                     LMGIPNLPDESVPEGVDETGNVEVRRWGAPRAFDFAVKDHVDLGAPLGLDFDTGAKLS
                     GSRFTFLRGPVARLHRALAQFMLDVHTLEHGYTECYTPYIVNREILEGTGQLPKFKED
                     MFWVLRGGDEQQAEQYLISTSEISLTNTVRESILKAEDLPVKMTAHSPCFRSEAGSAG
                     RDTRGMIRQHQFDKVEMVRIEHPERSMDALEEMTRHAEAILQKLGLPYRTMLLCGGDM
                     GFGSTKTYDLEVWLPAQDTYREISSCSNMGAFQARRMQARFRNAAGKPELVHTLNGSG
                     LAVGRTLVAVLENYQQADGSIVIPEILRPYMGGVAQLKP"
     gene            3234..3878
                     /locus_tag="VF453_21270"
     CDS             3234..3878
                     /locus_tag="VF453_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEX7690251.1"
                     /translation="MDYPTYDPTSPVTPLTEEELRALDELLQALPCDGAMTVDGLDGY
                     LTALLVGPPLLATLPTADWITAVWGGDGDVPPAPFASNQKRKRTTVLVLRHLRALQEA
                     IANSPDDWEPIFSVAETRTGEEFADASDWCLGFLAATDLAPEAWAPLFADPELGPGLA
                     TIALLGEGSTGGDGHAPEEAGLDEDDDLDDPHVRDQLSRAAADVVLALWARRAG"
BASE COUNT          604 a         1403 c         1394 g          512 t
ORIGIN      
        1 gcgcggggcc cgcgtgcgcg tcgccttggc gcgcgcgggc ggtggatcgt cggcgggcga
       61 cgggggcggt gcgggcggca ggatcgcctg ctcggcggcg ggcgggggcg ggtcgagggt
      121 aaaattcatg tttccaggtg cttgaacagc gtcgtgcagt tgcgtggcca tgcaccagtg
      181 tcccgccgcg gcgtgccgac gccgcgtgca aaaacgcccc caaacccgtg aagaccgtcc
      241 agcccgactc gttcctgacg ctgcattacc gcctgtgcgg ccccgatggc gcggaagtcg
      301 tcagcacgtt cgacgacaag cccgccacgc tgtcgctcgg cgccggccag ctcgcgcccg
      361 cgatcgaggc ctgcctcgtc ggcatggagg agggcgcgcg catgacctgg cacctcgagg
      421 cgggcgcggc ctacggccag cgcaaccccg agatgctcca gcgcgtcaag cgcagcctgc
      481 tcgcgcagca cggcgacccg gacgaggact accgtgtggg cgacgtcgtc gagttcccga
      541 cccccgacgg ccacggccgc tttgccggcg tcgtgcgcga ggcgggcgac gactggctgc
      601 tgttcgactt caaccatccc ctcgcgggcc agccggtcac cttcgaagcg caactcatcg
      661 gagtgatgaa ttgacgaccc ccccgaagcg cctgcggcgc ctccccccga gggggcgcac
      721 ccggcggacc ggcggagccg gatccgcggc tgccgctggg tcgcgccgca gtttcgcggg
      781 aggatcgcga tgagtgcgga actggaggcc aaggtcgtcg gcgaggtgct gctcgcgcag
      841 ccgcgcggct tctgcgccgg cgtcgaccgc gcgatcgaga tcgtcgaacg cgcgctcgag
      901 aagttcggcc ggccgatcta cgtgcgccac gagatcgtgc acaacacctt cgtcgtcaac
      961 gacctcaaga gcaagggcgc gatcttcatc gaggacctcg ccgaggtgcc gcccggcgcg
     1021 accctcgtgt tctcggcgca cggcgtgagc aaggccgtgc gcgacgaggc cgacgcgcgc
     1081 ggcttctcgg tcttcgacgc gacctgcccg ctggtgacca aggtgcacgt cgaggtctcc
     1141 aagctggcgc gcgagggcta cgagttcatc atgatcggcc acaagggcca tcccgaggtc
     1201 gagggcacga tgggccagct gaccgagggc atctacctcg tcgaggacgt gcccgacgtc
     1261 gagcgcgtgc atccgcgcga tccctcgaag ctcgccgtgg tcacgcagac cacgctgtcg
     1321 gtggacgacg cggccgagat cctcgccgcc gtcaagcgcc gcttcccgct gatccgcgag
     1381 cccaagaagc aggacatctg ctacgcgacg cagaaccgcc aggacgcggt caagttcatc
     1441 gcgcccaagg tggacatcgt gatcgtggtc ggcagcccga ccagctccaa ctccaaccgc
     1501 ctgcgcgagc tggcggagcg cctgggcacg ccggcctaca tggtcgacgc ggcgagcgac
     1561 ctgcagcccg cgtggttcga gggccgctcg cgcgtgggcc tgaccgcggg cgcgtcggcc
     1621 ccggacgtcc tcgtgcagca ggtcatcgac cgcctgaagg cgctcggcgc gatcagcgtg
     1681 cgcaacgtcg agggcgtcga ggagacggtc gagttcccgc tgccgctggg cctgggcgac
     1741 cgcagcatga acgagctgcg cgccgcgcgg gccggcgagg cgccgcgatc ttgacgctcg
     1801 ccgcctgagc ccgcgcacgg cgcggcgctc gccccgagga tgcccgccgc cgcgccgacc
     1861 ccgccggttc ggcgagcgcc ccgaccgaat cgacgaagaa ccacaccgag gactcccgcc
     1921 atgctcgaca tcaacctgct gcgccgcgac ctgcccgccg tcgtcgccgg cctcgaaaag
     1981 cgcaagaacc cgcagcccta cctggacgtc gccgcctaca cggcgctcga ggccgagcgc
     2041 aaggcggtgc aggtgcgcag cgaggaaggc caggcgcgcg tgaacgcgct gtccaggcag
     2101 atcggccagg ccaagggcaa gggcgaggac gcgtcggcgc tgatggccga ggtcgccgag
     2161 ctcaaggcgc agaaggccgc gggcgacctg cggctggagg agctgcaggc cgagctgtcg
     2221 acgatgctga tgggcatccc caacctcccg gacgagagcg tgccggaggg cgtcgacgag
     2281 accggcaacg tcgaggtgcg ccgctggggc gcgccgcgcg cgttcgactt cgcggtcaag
     2341 gatcacgtcg acctcggcgc cccgctgggc ctggacttcg acaccggcgc caagctctcg
     2401 ggctcgcggt tcaccttcct gcgcggcccg gtggcgcgcc tgcaccgcgc gctcgcgcag
     2461 ttcatgctgg acgtgcacac gctggagcac ggctacaccg agtgctacac gccctacatc
     2521 gtcaaccgcg agatcctcga gggcaccggc cagcttccga agttcaagga ggacatgttc
     2581 tgggtgctcc ggggcggcga cgagcagcag gccgagcagt acctgatctc gacctcggag
     2641 atctcgctca cgaacacggt gcgcgagtcc atcctgaagg ccgaggacct gcccgtgaaa
     2701 atgaccgcgc acagcccctg tttccgctcg gaagcgggct cggccggccg cgacacgcgc
     2761 ggcatgatcc gccagcacca gttcgacaag gtcgagatgg tgcgcatcga gcatcccgag
     2821 cgcagcatgg acgcgctcga ggagatgacg cgccacgccg aggccatcct gcagaagctg
     2881 ggcctgccgt accgcacgat gctgctgtgc ggcggcgaca tgggcttcgg ctcgaccaag
     2941 acctacgacc tggaggtctg gctgccggcg caggacacct accgcgagat cagctcgtgc
     3001 tcgaacatgg gcgcgttcca ggcgcggcgc atgcaggcgc gcttccgcaa cgccgcgggc
     3061 aagccggagc tcgtgcacac cctcaacggc tcggggctgg cggtcgggcg cacgctggtc
     3121 gcggtgctgg agaactacca gcaggccgac ggctccatcg tgatcccgga gatcctgcgg
     3181 ccgtacatgg gcggcgtcgc ccagctgaag ccctgataca gggagccggt accgtggact
     3241 atccgaccta cgaccccact tcgcccgtca cgccgctgac cgaggaggag ctacgcgcgc
     3301 tcgacgagct gctgcaggcg ctgccctgcg acggcgcgat gacggtggac ggcctggacg
     3361 gctacctcac ggcgctgctg gtcgggccgc cgctgctcgc gacgctgccg accgccgact
     3421 ggatcaccgc ggtctggggc ggggacggcg acgttccgcc cgcgcccttc gcgagcaacc
     3481 agaagcgcaa gcgcacgacg gtgctcgtgc tgcggcacct gcgcgcgctc caggaggcga
     3541 tcgcgaactc gcccgacgac tgggagccga tcttctcggt ggccgagacc cgcacgggcg
     3601 aggagttcgc cgacgcgagc gactggtgcc tcggcttcct tgccgcgacc gacctggcgc
     3661 ctgaagcatg ggcgccgctg ttcgccgatc cggagctcgg acccggcctc gcgaccatcg
     3721 cgctgctcgg cgaaggcagc accggcggcg acggccatgc gcccgaggag gccggcctcg
     3781 acgaggacga cgacctcgac gatccgcacg tgcgcgacca gctgagccgc gcggccgcgg
     3841 acgtcgtgct cgcgctgtgg gcgcgccgcg ccggttgacc gcggcggtgc cgatgcgttc
     3901 gcccacgtgg aac
//
LOCUS       DASVLN010000907         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_101947, whole genome shotgun sequence.
ACCESSION   DASVLN010000907 DASVLN010000000
VERSION     DASVLN010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101947"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VF453_21275"
     CDS             <1..282
                     /locus_tag="VF453_21275"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7690252.1"
                     /translation="TADSRHLAVGALEPRFWQAFCFAIGRPEWVDRHWSAGERPGGAA
                     AFETRAAVAALLATRPLADWLALTDPADCCVTPVLRPDEVERHEWFRGA"
     gene            327..1481
                     /locus_tag="VF453_21280"
     CDS             327..1481
                     /locus_tag="VF453_21280"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7690253.1"
                     /translation="MATNPYRMNRRRCLAVAGMLAACMVVAACTSPGSPATPGQPAGR
                     SLQELVDDRHVCHAALAVIHARHVASVTAAGAGGDCLSASGERDGKGSQPSGGPRADP
                     DAVFIAASLSKPVVAYAALELVDEGRLALDAPVMGYLPGGWQHRRKPLAASSPTDPAN
                     DPRLARVTIRMLLDHTSGLPNWSEGPPTLSFEPGGGWQYSSEGYVLLQRAVEAVAGEP
                     LDRVVATRVFEPLGMTRSALAWDDRLAPAYVAGRSADGHARAPRHFRVPVASTTLYTT
                     AGDYARFVAALLDDRPRLTEITASPVPVDERLGLSWGLGWGLEDDGGRRTMFQWGDFP
                     GYRALVMAVPASGDGFVLLTDSDEGLKIAPEVAARFLPGRHRLFDFRLVR"
BASE COUNT          236 a          800 c          804 g          293 t
ORIGIN      
        1 accgccgaca gccgccatct cgccgtcggc gcgctggagc cgcgcttctg gcaggccttc
       61 tgcttcgcga tcggccgccc cgagtgggtc gaccgccact ggtcggccgg cgagcgaccg
      121 ggcggcgccg cggcgttcga gacccgcgcc gccgtcgccg cgctgctcgc gacgcgaccg
      181 ctggccgatt ggctcgcgct gaccgacccc gccgactgct gcgtcacgcc ggtgctgcgg
      241 cccgacgagg tcgagcggca cgagtggttc cggggcgcct gatcgcgtcg gtcgcgtcgc
      301 gaagccggca tcgatccgca ctctccatgg cgacgaaccc ctaccgcatg aaccgccgtc
      361 gctgcctggc cgtggcgggc atgctggcgg cgtgcatggt ggtcgccgcg tgcacgtcgc
      421 cggggtcgcc cgcgacgccc ggccagccgg ccggccgctc gctgcaggag ctggtcgacg
      481 accgccacgt ctgccacgcc gcgctcgccg tgatccatgc gcgacacgtg gcctccgtga
      541 ccgcggcggg ggccggcggc gactgcctgt ccgcatccgg cgagcgggac ggcaagggct
      601 cgcagccatc cggcgggccg cgggccgatc ccgacgcggt gttcatcgcc gcgtcgttgt
      661 ccaagccggt cgtcgcgtac gccgcgctcg agctcgtgga cgaagggcgc ctcgcgctcg
      721 acgcgccggt gatgggctac ttgcccgggg gctggcagca ccggcgcaag cccctggcgg
      781 cctcgagccc gaccgacccg gcgaacgacc cgcggcttgc ccgcgtcacc atccgcatgc
      841 tgctcgatca cacctccggc ctgccgaact ggagcgaggg gccaccgacg ctgtccttcg
      901 agccgggcgg cggctggcag tactcgagcg agggctacgt gctgctgcaa cgcgccgtcg
      961 aggccgtggc cggcgagccg ctggatcgcg tcgtcgcgac gcgcgtgttc gagccgctgg
     1021 gcatgacgcg cagcgcgctc gcctgggacg accgcctcgc gcccgcctac gtggccgggc
     1081 gctcggccga tggccacgcc cgcgcgccgc gccacttccg cgtgccggtg gcctcgacca
     1141 cgctgtacac gaccgccggc gactacgcgc gcttcgtcgc ggcgctgctc gacgaccgcc
     1201 cccggctgac tgagatcacg gcgtcccccg tgccggtcga cgagcggctc ggcttgtcgt
     1261 ggggactcgg ctggggcctc gaggacgacg gcggccggcg cacgatgttc cagtggggcg
     1321 atttcccggg ctaccgcgcg ctcgtgatgg ccgtgccggc gagcggcgac ggcttcgtgc
     1381 tcctcaccga cagcgacgaa ggcctgaaga tcgcgccgga ggtcgccgcg cggttcctgc
     1441 ccgggcggca ccggctgttc gacttccggc tggtgcgctg aaggacgcga cggccgtcgc
     1501 gaccgaagcg cctgtgggcc ccgcgcgcct ggcgtcgctt ggcgacgcgc gccgtatgcg
     1561 tcccgcgact cgccgcgtcg ggtcgaacat ccacgcgcgg gccgccgggc cgggtcattg
     1621 cgtcccggag gtccggcgat ggatcgcgga gagtcggcgc ctcgtctttc ggaggcatcc
     1681 acggctctcc aggccaggac gcgtcgtcgc aaggacggac gcttccccgg ggcgggctcc
     1741 ggcgtcctcg ccggcagacg catcgtcgcc ccgaaagggt gcgcgcgtcg ttcggcgagc
     1801 caggccgtcc tggcaccaga acccgtcgtc tcgccgtggc gaggcagcgt ccgctctccg
     1861 ggagcgatcg tcggcttttt gagaggcatc gtcggccctc cgagaggcat cgtcgactct
     1921 ccgggccgga aggcgtgccg ccggggggcg ccggcccgcg gtgcgagggc cgtgcttgcc
     1981 gcctccggtg ggaaggtggc gcgacggaga gctgcaccat gaagaacgcc tccggcgctg
     2041 gcgcgcggga ggcgtcgatg caaagggggt cggcgcgtca gcgcttgccg gagcccttgc
     2101 cggaggtcag cgccgcgcgc tgcggctgct gcg
//
LOCUS       DASVLN010000908         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_159092, whole genome shotgun sequence.
ACCESSION   DASVLN010000908 DASVLN010000000
VERSION     DASVLN010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159092"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VF453_21285"
     CDS             complement(<1..340)
                     /locus_tag="VF453_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX7690254.1"
                     /translation="MSRPAIRFHEFPLSGHSHRVAQMLAMLELPVERVAVDLAAGDQR
                     RPGFLALNRFAQVPVIEDGDVVVADSNAILVYLAKRYGAGTGWLPEDPAGAARVQRWL
                     SVAAGPVAFGP"
     gene            complement(414..1325)
                     /locus_tag="VF453_21290"
     CDS             complement(414..1325)
                     /locus_tag="VF453_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7690255.1"
                     /translation="MDRIQEMTVFVAVAEAGSFAGAARALGVSAATVTRGVAQLERRL
                     GSLLVVRNTRQLRLTEAGTRFADDARRLLQELQEAEESAAGVHAAPRGLLTITAPQVF
                     GDLHVVPAMTAYLAGHPGVDIRALLVDRVVALLEEGVDVAVRIGELPDSSMTAVPVGE
                     IRRVVVASPDYLARHGTPAHPDELAARATISAHGRQAGHHWRFRVDGRLHDVAVRSRL
                     TVNSFRAAIHAATTGAGLTQVASYQVAEAVAAGALRAVLQDFEVEPRPVHLVYTEGRR
                     GSRKVRSFVDFCVPRLRAALSGPARMN"
     gene            complement(1304..2269)
                     /gene="yedA"
                     /locus_tag="VF453_21295"
     CDS             complement(1304..2269)
                     /gene="yedA"
                     /locus_tag="VF453_21295"
                     /inference="COORDINATES: protein motif:HMM:NF008432.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HEX7690256.1"
                     /translation="MSRLRTGASTLLVSLALVYVLWGSTYLGIRIAIEGLPPLVLSAM
                     RNLVAGALLFAWAMSRGAPWPTPRQWRNGALMGFMMLSIGNGFVCLAERSVPTGLAAL
                     VVGSGPVFAILFAWGFGTRPRALEWAGVLLGFIGVAMLNLDLRAAASPGGILLLLVAT
                     ASWSMATILQPRLDLPKGGASAGVQLLGGGVVLVAMAALHGESLPAHVPMRSWLALAY
                     LIVFGSLIAYSAFVYVVNHSRPALAMSYAYVNPIVAVTLGALFADEPVTLPLLGAMAV
                     ILVAVALVVLAHNRKPPPKDEAKDVSSPLSRTDGQGARAWTGSRR"
BASE COUNT          390 a          981 c         1010 g          301 t
ORIGIN      
        1 ccggcccgaa ggccaccggc ccggctgcca ccgagagcca gcgctgcacg cgcgcggcgc
       61 cggcgggatc ctccggcagc cagcccgtgc ccgcgccgta gcgcttggcc aggtagacga
      121 ggatggcgtt ggagtcggcg accacgacgt cgccgtcctc gatcaccggc acctgcgcga
      181 agcggttgag cgcgaggaac ccgggccggc gctggtcgcc ggcggcgagg tcgactgcga
      241 cgcgctcgac cggcagctcc agcatcgcga gcatctgcgc gacgcggtgg ctgtggccgg
      301 agagcgggaa ttcgtggaag cggatggcgg gacgggacat ggcgcgggac tcgctgcggt
      361 gagggagccc gcatcgtgcc gccgcccgcg gcgcgaggga acggccggcc cgctcaattc
      421 atccttgcag gacctgaaag ggcggcccgc aggcgcggca cgcagaagtc gacgaacgaa
      481 cgcaccttgc gcgagccgcg gcggccttcg gtgtacacga ggtgcaccgg acgcggctcg
      541 acctcgaagt cctgcagcac cgcccgcagc gcacccgccg cgacggcctc ggcgacctgg
      601 taggacgcga cctgcgtcag gccggcgccg gtcgtcgcgg catggatcgc ggcgcggaag
      661 gagttgaccg tgagccggga gcgcaccgcc acgtcgtgga gccgcccgtc gacgcggaag
      721 cgccagtggt ggccggcctg gcggccgtgg gcggagatgg tcgcgcgcgc ggcgagctcg
      781 tcgggatgcg ccggcgtgcc atggcgcgcg aggtagtccg gcgaggcgac gaccacgcgc
      841 cggatctcgc ccacgggaac ggcggtcatc gacgagtcgg gcagctcgcc gatgcgtacc
      901 gcgacgtcga cgccctcctc cagcagcgcg acgacgcggt cgacgagcag ggcgcggatg
      961 tcgacgccgg gatgcccggc caggtaggcc gtcatcgcgg gcacgacatg gaggtcgccg
     1021 aagacctgcg gcgcggtgat cgtcagcagc ccgcgcggcg cggcgtgcac gccggcggcg
     1081 gactcctcgg cctcctgcag ctcctgcagc aggcggcggg cgtcgtcggc gaagcgggtg
     1141 ccggcctcgg tcaggcgcag ctggcgcgtg ttgcgcacga ccagcaggct gcccaggcgg
     1201 cgctcgagct gcgcgacgcc ccgcgtcacc gtcgccgccg acacgcccag cgcgcgcgcc
     1261 gcgccggcga agctgccggc ttccgcgacg gcgacgaaga ccgtcatctc ctggatcctg
     1321 tccacgcgcg cgctccttgc ccgtcggtgc gggaaagagg cgagcttacg tccttggcct
     1381 cgtccttcgg cggcggcttg cggttgtgcg cgagcaccac cagcgccacc gccaccagga
     1441 tgaccgccat cgcccccagc agcggcagcg tgacgggctc gtcggcgaac agcgcgccca
     1501 gcgtcaccgc gacgatcggg ttgacgtagg catagctcat ggccagcgcg ggccgcgagt
     1561 ggttgacgac gtagacgaac gcggagtagg cgatcagcga gccgaacacg atgaggtagg
     1621 cgagcgccag ccagctgcgc atcggcacgt gcgccggcag ggactcgccg tgcagcgccg
     1681 ccatcgcgac gaggaccacg ccgccgccca gcagctgcac gccggccgag gcgccgccct
     1741 tgggcaggtc caggcgcggc tgcaggatgg tcgccatcga ccacgaggcc gtcgccacga
     1801 gcagcagcag gatgccgccg gggctcgcgg cggcgcgcag gtcgaggttg agcatcgcca
     1861 cgccgatgaa cccgagcagc acgccggccc actccagcgc gcgcgggcgc gtgccgaaac
     1921 cccacgcgaa caggatcgcg aacacgggcc cgctgccgac cacgagcgcc gcgaggcccg
     1981 tgggcaccga gcgctcggcc aggcagacga agccgttgcc gatgctcagc atcatgaagc
     2041 ccatcagcgc gccgttgcgc cattgccgcg gcgtcggcca cggcgccccg cgcgacatcg
     2101 cccacgcgaa cagaagcgcc cccgcgacga ggttgcgcat cgccgacagg accagcggcg
     2161 gcaggccctc gatcgcgatg cggatgccga ggtaggtcga gccccagagg acgtagacga
     2221 gcgcgagcga gacgaggagc gtcgaggcac cggtgcggag gcgggacatg gtcggacggg
     2281 ggaaggcatg cggcgggagc atcgactcta gtggcccgcc cgcgcgcgtg gcgtgcgttt
     2341 ttctcatcgg ggtcatgcgc aaagcgcctg cgtcgacgcg cggccctgcg gcgcgggcgc
     2401 gacgaagttc cgtgtcccgg cgagacattg ccttgtccgc gcaggacatc gccgcgccgc
     2461 gccgcctgcc cggccgtgcg cattgcatag actccggcgt ccgcacagtc gcgctggagt
     2521 gtcccgatgt cctggagccg ccttccgtcc atccccctgc cgatccgtcc ggcccgtgtg
     2581 tcccgtgtgg cccgggtggc ccacctggca gccggcgccg cgctcgccct cgccgcctgc
     2641 ctgccggccg gcgccgcgcg cgcggccgat ccggcgcaga cc
//
LOCUS       DASVLN010000909         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_208746, whole genome shotgun sequence.
ACCESSION   DASVLN010000909 DASVLN010000000
VERSION     DASVLN010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208746"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VF453_21300"
     CDS             <1..184
                     /locus_tag="VF453_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7690257.1"
                     /translation="AGIAVQAGTLLDAPIARGELVELLAGWTLPSRAVHVVRLPEARP
                     SAKLRTFVDFAVERLG"
     gene            225..1010
                     /locus_tag="VF453_21305"
     CDS             225..1010
                     /locus_tag="VF453_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7690258.1"
                     /translation="MSPNAHIRLDPTDADGIAALRISNPGKMNALTLGMWRELRAVFD
                     GLRQSDAPPRVIVVRGEAGEFVAGADIEEFPQFRFEPETLAAYHEDVVAPALHAMLDC
                     DVPLVAHIEGACIGGGLEIAACCDLRLCEPASRFGVPIARLGFPMAPAEVEIVSHIVG
                     QTLLRELLVEARLLHAAEAHARGLVTRVIDSERLEAEVRRTAVHIASLSPQAMRLNKR
                     VLRTFARPSFSDAEARAAHYDYAASDEYREGVQAFIEKRPAKF"
     gene            complement(1021..1773)
                     /locus_tag="VF453_21310"
     CDS             complement(1021..1773)
                     /locus_tag="VF453_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX7690259.1"
                     /translation="MRRLVRLLQIAYAVAAYALFLATMLATIAFVLDLGLPWSVDAPA
                     VGSAAAAIVVDLALVLVFGLQHSAMARPGFKRAWTRVVPAPVERATYVLASSLALALL
                     IVAWRPLPGVLWDARGTPVGAVLLAAGAGGWLLLVAASFLVSHWELFGLVQAWRGLGG
                     VDARAAGAMPELSERGLYGVVRHPIMLGFLVAFWCAPRMSTGHLLFAGACSAYILVGT
                     RLEERDLLAHFGAAYARYRERVPMLLPRPWRR"
     gene            1895..>2999
                     /locus_tag="VF453_21315"
     CDS             1895..>2999
                     /locus_tag="VF453_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7690260.1"
                     /translation="MTASAPSGNLYAALRAHFPADLDGIAIETADAPASRADAHFEPL
                     HYSWRDLERGSARIAALLADLGLAPGARVVVHADKSVEAVMLYLATLRAGLVHVPLNT
                     AYQADELAYFIENAAPALVVCAPRHFPWVSRLAFARGVRTVLTLGDDRTGSLLERAAQ
                     IDERFEDVPRAGSDTAVIVYTSGTTGRSKGAQLTHGNLLANAEVLHDAWQWRDGRSIA
                     DDGGERAGRDVLIHALPIFHVHGLFVALHGALRAGARMLWFHRFDPLATIARLPGASV
                     FMGVPTLYVRMLAQPALTREACASMRLFVSGSAPLLADTFEAWRARTGHAILERYGMS
                     ETVMLTSNPYRPESARRAGTVGRALPGVGVRVRD"
BASE COUNT          405 a         1146 c         1084 g          364 t
ORIGIN      
        1 cgcgggcatc gccgtgcagg cgggaacgct gctcgacgcc ccgatcgcgc gcggcgagct
       61 cgtcgagctg ctcgccggct ggacgctgcc gtcgcgcgcg gtgcacgtcg tgcgcctgcc
      121 cgaggcgcgg ccgagcgcga agctgcgcac cttcgtcgac ttcgccgtcg aacgtctcgg
      181 ctgacgcgag tacagtggcc gcttctcccc acgaaggagc cgccatgtcc ccgaacgccc
      241 acatccgcct cgacccgacc gacgccgacg gcatcgccgc gctgcgcatc tccaacccgg
      301 gcaagatgaa cgcgctcacg ctggggatgt ggcgcgagct gcgcgcggtc ttcgacggcc
      361 tgcggcagtc cgatgcgccg ccgcgcgtga tcgtcgtgcg cggcgaggcc ggcgagttcg
      421 tcgccggcgc cgacatcgag gagttcccgc agttccgctt cgagcccgag acgctcgcgg
      481 cgtaccacga ggacgtcgtc gcgccggcgc tgcacgcgat gctcgactgc gacgtcccgc
      541 tggtcgctca catcgagggt gcctgcatcg gcggcgggct ggagatcgcg gcctgctgcg
      601 acctgcgcct ttgcgagccc gccagccgct tcggcgtgcc gatcgcgcgc ctggggttcc
      661 cgatggcgcc ggcggaggtc gagatcgtct cccacatcgt cggccagacg ctgctgcgcg
      721 agctgctggt cgaggcccgc ctgctgcacg cggccgaggc gcatgcacgc gggctcgtca
      781 cccgcgtgat cgacagcgag cgcctcgaag ccgaggtgcg gcgcaccgcc gtccacatcg
      841 cctcgctgtc gccgcaggcg atgcgcctga acaagcgcgt gctgcgcacc ttcgcgcgcc
      901 cgtcgttcag cgacgccgag gcccgcgccg cgcactacga ctacgccgcc tccgacgagt
      961 accgcgaggg cgtgcaggcg ttcatcgaga agcggccggc gaagttctga gtaaccggcc
     1021 ctatcgccgc cagggccgcg gcagcagcat cggcacgcgc tcgcggtagc gcgcgtaggc
     1081 ggcgccgaag tgcgcgagca ggtcgcgctc ctcgagccgc gtgcccacga ggatgtaggc
     1141 gctgcaggca ccggcgaaga gcaggtggcc ggtcgacatg cgcggcgcgc accagaacgc
     1201 gacgaggaag cccagcatga tcgggtggcg cacgacgccg tagaggccgc gctcgctgag
     1261 ctcgggcatc gccccggcgg cgcgcgcgtc gacgccgccc aggccgcgcc aggcctgcac
     1321 gaggccgaac agctcccagt ggctcacgag gaaggatgcc gccaccagca gcaaccagcc
     1381 ccccgcgccc gctgccagca gcacggcgcc cacgggcgtg ccgcgcgcgt cccacagcac
     1441 gcccggcagc ggccgccagg cgacgatcag cagcgccagc gcgaggctgg acgcgagcac
     1501 gtaggtcgcg cgctcgacgg gcgccggcac cacgcgcgtc cacgcgcgct tgaacccggg
     1561 tcgcgccatc gcgctgtgct gcaggccgaa gacgagcacc agcgccaggt cgacgacgat
     1621 cgccgccgcg gccgatccga ccgccggcgc gtcgaccgac cacggcaggc ccaggtcgag
     1681 gacgaacgcg atcgtcgcga gcatggtcgc gaggaacagc gcgtaggcgg cgaccgcgta
     1741 ggcgatctgc aggaggcgga cgaggcggcg catcgggcgg cggcgagggg gcggcgcgcg
     1801 atgctcgcac gcggcggcgc cgcgcgccac cggcaagacc cgcgggcggc gaggctgcac
     1861 aatgccggca gtccgtcctt ccgcacgtcc ggccatgacc gcttccgcgc cttcgggcaa
     1921 cctgtacgcc gcgctgcgcg cccatttccc cgccgacctc gacggcatcg ccatcgagac
     1981 cgccgacgcg cccgcctcgc gcgccgacgc ccacttcgag ccgctgcact actcgtggcg
     2041 cgacctcgag cggggcagcg cgcgcatcgc cgcgctgctc gcggacctcg gcctggcgcc
     2101 gggcgcgcgc gtcgtggtgc acgccgacaa gtcggtcgag gcggtgatgc tctacctcgc
     2161 gacgctgcgc gcggggctcg tccacgtgcc gctgaacacg gcctaccagg ctgacgagct
     2221 cgcatatttc atcgagaacg ccgcccccgc gctggtggtg tgcgcgcccc gccacttccc
     2281 atgggtgagc cggctcgcgt tcgcgcgcgg ggtgcgcacg gtgctcacgc tcggcgacga
     2341 tcgcacgggc agcctgctcg agcgcgcggc gcagatcgac gagcgcttcg aggacgtgcc
     2401 gcgcgccggc tcggacaccg cggtcatcgt ctacacgagc ggcaccaccg gccgcagcaa
     2461 gggcgcgcag ctgacgcacg gcaacctgct cgccaacgcc gaggtgctgc acgacgcctg
     2521 gcaatggcgc gacggccgca gcatcgccga cgatggcggc gagcgcgccg ggcgcgacgt
     2581 gctgatccac gcgctgccga tcttccacgt gcacggcctg ttcgtcgcgc tgcacggcgc
     2641 gctgcgggcc ggcgcgcgga tgctgtggtt ccatcgcttc gacccgctcg cgacgatcgc
     2701 gcgcctgccc ggggcgagcg tgttcatggg cgtgccgacg ctgtacgtgc gcatgctggc
     2761 ccagccggcg ctgacgcgcg aggcctgcgc gtcgatgcgc ctgttcgtga gcggctcggc
     2821 cccgctgctg gccgacacgt tcgaggcctg gcgcgcacgc acgggccacg cgatcctcga
     2881 gcgctacggc atgagcgaga ccgtgatgct cacctccaac ccgtaccgcc ccgagtccgc
     2941 gcgccgcgcc ggcaccgtcg gccgtgcgct gcccggcgtg ggagtgcgcg tgcgcgacg
//
LOCUS       DASVLN010000910         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_45886, whole genome shotgun sequence.
ACCESSION   DASVLN010000910 DASVLN010000000
VERSION     DASVLN010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45886"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..962
                     /locus_tag="VF453_21320"
     CDS             222..962
                     /locus_tag="VF453_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7690261.1"
                     /translation="MTPTHIAVLDDEADITQLLGSYLASQGYRVTQLHLGRELLALMA
                     ADPPALVLLDLGLPGEDGFAIARQLREHFRVGLVIVTGRGDSVDKVVGLEIGADDYVT
                     KPFDLRELAARVKAVLRRLEPAKASAAPPVAAPGPAPSRERFRFAGWQFDTAARRLLD
                     PQGVEVVLTTGEFDLLAVFVRHPGRVLSRDFLLEQTRGRDAGPFDRTIDVQVGRLRRK
                     MEADSERPELIKSVRGAGYVLVPEVAAG"
     gene            977..>2462
                     /locus_tag="VF453_21325"
     CDS             977..>2462
                     /locus_tag="VF453_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7690262.1"
                     /translation="MRRELAPALALSGFDEGNVFRSMVSASPDAMLLVDAGGRIVLSN
                     PAAEALLGYSADEFAGMPVEDLVPDAIRPRHAAWRESYARVPRSRPMGTQMDLVARRK
                     DGGEVMVEIALSPLQDHGLPFVVASIRGIGAYPRVRQALQRERYSDRLAQLGRLAVDE
                     RDARRLIDHACGLAVDALELESAVVCLLADGGRDFVIAGGHRLLPEQDVGRRVPADPA
                     ALPGYLIAHPRPLALADYRTEQRFALPGYLVAAGLVAGLVAPLVDRGRVFGSLALHAR
                     AARDFGEDDMRFVESLCSLLATCLQRAQTEEALSHSQRLETVGQLTGGIAHDFNNLLT
                     VIKGNLQILEDAPAVAADANAQALLGAAGRATRRAAELTAKLLAFSRRQMLQPAAIDL
                     AAMLASLADMLRRTVDQRVRIEVDCPADCPAVFADPVQLESAMLNVAVNARDAMPDGG
                     RLLFRARAVDRLPGDADAPAPAGGDAPGGYVAIAISDTGTGMPDE"
BASE COUNT          285 a          946 c          903 g          328 t
ORIGIN      
        1 aggatgcggc ggtacatggc ggctcctggc gcgcgatggc gcgcggtggc gatgagggcg
       61 agtctggcga ccggcggccg cccggcgctt gcgctggcgc aaggcggccc ggtggcgcgc
      121 cggccgaggc cgacaagtcc ccgctccccg ctttcccgac gcctgcggcg gccgggaacg
      181 cggtacgctc gcgcatcggc gttcgcccca cccgccatcc gatgaccccg acccacatcg
      241 ccgtcctcga cgacgaagcc gacatcaccc agctgctcgg cagctacctc gccagccagg
      301 gctatcgcgt cacccagttg cacctggggc gcgagctgct ggcgctcatg gcggccgatc
      361 cgccggcgct ggtgctgctc gatctcgggc tgccgggcga ggacggcttc gccatcgccc
      421 gccagctgcg cgagcacttc cgggtcgggc tggtgatcgt caccgggcgc ggcgactccg
      481 tcgacaaggt cgtgggactc gagatcggcg ccgacgacta cgtcaccaag ccgttcgacc
      541 tgcgggagct cgcggcgcgc gtgaaggccg tgctgcggcg cctggagccg gcgaaggcgt
      601 ccgcggcgcc gcccgtcgcc gcgcccgggc cggcgccgtc gcgcgagcgc ttccgcttcg
      661 ccggctggca gttcgacacc gccgcccgcc ggctgctcga tccgcagggc gtcgaggtgg
      721 tgctgacgac cggcgagttc gacctgctgg cggtgttcgt gcgccacccg gggcgcgtac
      781 tgtcgcgcga cttcctgctc gaacagacgc gcggccgcga cgccggcccg ttcgaccgca
      841 cgatcgacgt gcaggtcggc cgcctgcgcc ggaagatgga ggccgattcc gagcggcccg
      901 agctgatcaa gtccgtgcgc ggcgccggct acgtgctggt gcccgaggtc gcggcgggct
      961 gaaaggggaa tcgacgatgc gccgcgagct cgcccccgcg ctggccctgt ccggcttcga
     1021 cgagggcaac gtcttccgct cgatggtgtc cgcgtcgccc gacgcgatgc tgctcgtgga
     1081 cgccggcggc cgcatcgtgc tgagcaatcc cgccgccgag gcgctgctgg gctactccgc
     1141 cgacgagttc gccggcatgc ccgtcgagga tctcgtgccc gacgcgatcc gcccccggca
     1201 cgccgcctgg cgcgagtcct acgcgcgcgt cccgcgctcg cggccgatgg gcacgcagat
     1261 ggatctcgtc gcgcgccgca aggatggcgg cgaggtgatg gtcgagatcg cgctcagccc
     1321 gctgcaggac cacgggctgc cgttcgtcgt cgcgtccatc cgcggcatcg gcgcctaccc
     1381 gcgcgtgcgc caggcgctgc agcgcgagcg ctacagcgac cgcctcgcgc agctggggcg
     1441 gctcgccgtc gacgaacgcg acgcgcggcg cctgatcgac cacgcctgcg gcctggccgt
     1501 ggatgcgctg gagctcgagt cggccgtcgt ctgcctgctg gccgacggcg ggcgcgactt
     1561 cgtcatcgcc ggcggccacc ggctgctgcc ggagcaggac gtcggccgcc gggtgccggc
     1621 cgatcccgcc gccctgccgg gctacctgat cgcccatccg cgacccctgg cgctggcgga
     1681 ctaccggacg gagcagcggt tcgccctccc cggctacctg gtcgcggcgg gcctggtcgc
     1741 cggcctcgtg gcgccgctcg tcgaccgcgg tcgggtgttc ggctcgctgg ccctgcacgc
     1801 ccgcgcggcc cgcgacttcg gcgaggacga catgcgcttc gtcgagtcgc tgtgcagcct
     1861 gctcgcgacc tgcctgcagc gcgcgcagac cgaggaggcg ctgagccact cgcagcgcct
     1921 ggagacggtc ggccagctga ccggcggcat cgcgcacgac ttcaacaacc tgctgacggt
     1981 gatcaagggc aacctgcaga tcctcgagga tgcgccggcc gtcgcggcgg acgcgaacgc
     2041 gcaggcgctg ctgggcgccg cgggccgggc gacgcgacgc gcggcggagc tcacggccaa
     2101 gctgctcgcg ttctcgcgcc ggcagatgct gcagccggcg gccatcgacc tggccgcgat
     2161 gctcgcgtcg ctggccgaca tgctgcgccg caccgtcgac cagcgcgtcc gcatcgaggt
     2221 ggactgcccg gccgactgcc ccgcggtctt cgccgacccg gtgcagctgg agtccgcgat
     2281 gctcaacgtc gccgtcaacg cgcgcgacgc gatgcccgac ggcggccgtc tcctgttccg
     2341 cgcgcgggcg gtcgaccgcc tgcccggcga cgccgacgcc ccggcgcccg ccggcggcga
     2401 cgcgcccggc ggctacgtcg cgatcgcgat ctccgacacc ggcaccggca tgcccgacga
     2461 gg
//
LOCUS       DASVLN010000911         6271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_45972, whole genome shotgun sequence.
ACCESSION   DASVLN010000911 DASVLN010000000
VERSION     DASVLN010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45972"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VF453_21330"
     CDS             complement(<1..862)
                     /locus_tag="VF453_21330"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7690263.1"
                     /translation="MRPDDAWLVTGASSGIGRALARRLAARGQAVWLAGRDVAAMRAL
                     RDGLRARHPGAAVEVDGLDLASRQSIEAGVERWASRGLRFAVLVNNAGVSMSRFGRCE
                     DGFEWTWATNVRGPYLLSRALAGRGLFADGAQVLNVASSCHARRLDGRCFERYGRLGG
                     ADAYLQSKLCLILQTRALGAEPGQLRFNAVHPGIVRSRLGRDGRVANLVGRAVGLFLP
                     PADRAARVLEQVAERVARCGLNRAWLDAGEPAELGYDGDLDADTARVMAALAADVVPG
                     GPATASAAGQR"
     gene            complement(852..1727)
                     /locus_tag="VF453_21335"
     CDS             complement(852..1727)
                     /locus_tag="VF453_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690264.1"
                     /translation="MARPSPRALRAIAVAGAAAIVLAYRLWPDPYTHAARIALFVSVL
                     ANLVAVGARSALDFRRRRRAGERRIDAARQSMPPALRRLLVMEFNIYREGFCTVLRLT
                     PPSVARMRRAPAGRAFTAMKGSFSSVFLPLVVVGCVVDTPLLHLYIHRAAPPASRDGL
                     HALVVAATMLGLVWAIGDRSAVRHMRHVVAPDVVMLNAGFRRNLAIPRDWIAEVGPVA
                     VSLREHVRAAVANHEMLLVSHMDAPNVLISLKASAFGHSIDIARPQARIRRIGVYVDE
                     PWAFAQQLAPSGHAT"
     gene            complement(1727..2638)
                     /locus_tag="VF453_21340"
     CDS             complement(1727..2638)
                     /locus_tag="VF453_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690265.1"
                     /translation="MARFLARDACAAPISLDEYIAYCDEHLAPDDEDNIFAGAAMLQA
                     LALDRRMLVDKLNDYFRQDDELNDLSTYSSMTMMLALRPKYFIRANTWVRPRAYAADD
                     AWENTLYAYDYAHNHNFSLLTVGYHGPGYRTDIYRLDDPSAITGYEGEKVSLAYEGRY
                     QLGRGDVLLYERVRDVHVQLPPDGLSISLNLMPLDSRLCLTEQFSFDVQAGTIASLVG
                     SQVAQQSDLLRMAACVGDGDTAELLLRLARRHSNQSTRLAAMQSAVMLLPSEAEKIWE
                     IGLDDKSEIVRQAARGAVLSTTSPMAL"
     gene            complement(2652..3554)
                     /locus_tag="VF453_21345"
     CDS             complement(2652..3554)
                     /locus_tag="VF453_21345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690266.1"
                     /translation="MNDRRGAAPSNYHFRHDPDALPILSRLMAASARIDSGAAEGVFA
                     LRAHFDDLVASREIAELLRTEVRHLDEDFGHVPSGFSGGTMVLAQSEAVAITATLMDA
                     RHGASPHIYSPVADAIYGNLGPGIFPVCVHERPEGADILTLDRTQRLALRPCREIAPG
                     GTFVVRAARDVPEYRCAERTLVLKLTCRSGPPLVWVYDKASGLPRMASSGSLAASRMQ
                     MMLRTLVALHGDDGDAAARARSADAIERLAGHDVHFLRWTALQSLCALDGERGLRRLR
                     DARADPHPHVAHAAARALEKLNIH"
     gene            complement(4192..5286)
                     /gene="apbC"
                     /locus_tag="VF453_21350"
     CDS             complement(4192..5286)
                     /gene="apbC"
                     /locus_tag="VF453_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEX7690267.1"
                     /translation="MPVSDSALAAALAAVVDPHTSQPYTAGRQLKKLRADADGRVAVE
                     VELGYPAKSQFAAVREALAAAARGVAGVTAVEVAVSSKVVAHQVQRGVALLPGVANVV
                     AVSSGKGGVGKSTTAVNLALALAAEGARVGLLDADIYGPSLPLMMGIEGRPESADGKT
                     MEPLANHGVKVMSIGFLVDPEQAVVWRGPMATQALDQLLRQTNWGELDYLVVDMPPGT
                     GDIQLTLSQRVPLTGAVIVTTPQDIALLDARKGLNMFEKVGVPILGIVENMAVHVCTN
                     CGHVEHIFGAEGGKRMAAQYGVPYLGALPLTLAIREQTDSGTPTVVADPDGEIAALYK
                     AVARQVAVVVAAKAKDFSAKFPTITVSKGT"
     gene            5465..>6271
                     /locus_tag="VF453_21355"
     CDS             5465..>6271
                     /locus_tag="VF453_21355"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7690268.1"
                     /translation="MELCPLVKVQHRIKLGTPLPFSIRDGQSKLLLAKDQVVKDEEQL
                     EALMDRGAYVNLDELRGARAEIYNAPPELLPSLWRKLADQLSRTLVGMPSPQAPQALD
                     EAARYLAALAERAPDVAIFLLVRPDPTSPVRYGVMRSLHAAVAAFLIALRLAWPTERC
                     LTLVKAAFTMNLGMLDLQERLSNQERALNPGQRQAIHAHPLTSVASLKDSGVADADWL
                     EAVAQHHERPGGAGYPAGLAQVGEMACALRTVDVFTAKLAGRAGRAPLAPT"
BASE COUNT          886 a         2266 c         2296 g          823 t
ORIGIN      
        1 ccctctgtcc cgccgccgac gcggtcgccg ggccgccggg aacgacgtcc gccgccagcg
       61 ctgccatcac ccgcgcggtg tcggcgtcga ggtcgccgtc gtagccgagc tccgccggct
      121 cgccggcatc cagccaggcg cggttcaacc cgcatcgcgc cacccgctcg gcgacctgct
      181 cgagcacccg tgccgcccgg tcggccggcg gcaggaacag gccgaccgcg cgccccacca
      241 ggttcgcgac ccggccgtcg cgccccaggc ggctgcgcac gatgcccggg tggacggcgt
      301 tgaagcgcag ctgcccaggc tccgcgccca gcgcgcgcgt ctgcaggatc aggcagagct
      361 tgctctggag gtaggcgtcg gcgcccccga ggcggccgta gcgctcgaag cagcgcccgt
      421 cgagccgccg cgcatggcag gacgacgcga cgttgagcac ctgcgcaccg tcggcgaaga
      481 ggccccgtcc ggcaagggcg cgcgacagca ggtaaggccc gcggacgttg gtggcccacg
      541 tccattcgaa gccgtcctcg cagcgcccga agcgcgacat cgagacgccg gcgttgttga
      601 ccagcaccgc gaaccgcagg ccgcgcgatg cccagcgctc gacgcccgcc tcgatcgact
      661 gccggctcgc gaggtcgagg ccatcgacct ccaccgccgc gcccggatgg cgcgcccgca
      721 gcccgtcgcg cagcgcccgc atcgccgcga cgtcccgccc ggcgagccac accgcctgcc
      781 cgcgggcggc caggcgtcgc gcgagcgccc gcccgatgcc cgaggacgcc ccggtcacca
      841 gccacgcgtc gtcaggtcgc atggccggag ggcgcgagct gctgggcgaa cgcccagggc
      901 tcgtcgacgt agacgccgat ccgccggatg cgcgcttgcg ggcgcgcgat gtcgatcgag
      961 tggccgaagg ccgaggcctt cagcgagatc agcacgttgg gcgcgtccat gtggctcacc
     1021 agcagcatct cgtggttcgc gaccgcggca cgcacgtgct cgcgcaggct gaccgcgacg
     1081 gggccgacct cggcgatcca gtcgcgcggg atggccaggt tgcggcggaa gccggcgttg
     1141 agcatcacca cgtccggcgc gaccacgtgg cgcatgtgcc gcacggcgct gcggtcgccg
     1201 atggcccaga ccagcccgag catcgtcgcc gccacgacga gcgcgtgcag cccgtcgcgg
     1261 ctggcgggcg gcgccgcgcg atggatgtac aggtgcagca gcggggtgtc gaccacgcag
     1321 ccgacgacga cgagcggaag gaagacggac gagaacgacc ccttcatcgc cgtgaatgcc
     1381 cgccccgccg gcgcgcggcg catgcgcgcg acgctgggcg gtgtcaggcg caggacggtg
     1441 cagaagcctt cgcgatagat gttgaactcc atcacgagca gccgccgcag cgcgggcggc
     1501 atcgattgcc gggctgcgtc gatccgccgc tcgccggcgc gccgccggcg gcggaagtcg
     1561 agcgccgagc gtgcgccgac ggccacgagg ttggcgagca ccgacacgaa cagcgcgatg
     1621 cgcgccgcgt gggtgtacgg gtcgggccag aggcggtacg cgaggacgat ggccgccgcc
     1681 cccgccacgg cgatcgcccg caaggcgcgc gggctcggac gagccatcac agcgccatcg
     1741 gcgacgtcgt cgacagcacg gccccgcgtg ccgcctggcg cacgatctcg ctcttgtcgt
     1801 cgaggccgat ctcccagatc ttctcggcct cggaaggcag cagcatgacc gccgactgca
     1861 tcgccgcgag ccgggtgctc tggttgctgt gccggcgcgc gaggcgcagc agcagctccg
     1921 cggtgtcgcc gtcgcccacg caggcggcca tgcgcagcag gtcgctctgc tgcgcgacct
     1981 ggctgccgac cagcgaggcg atcgtgcccg cctggacgtc gaacgagaac tgctccgtca
     2041 ggcacaggcg cgagtcgagc ggcatcaggt tcagcgagat cgacagcccg tcgggcggca
     2101 gctggacgtg cacgtcgcgg acgcgctcgt agagcagcac gtcgccgcgg cccagctggt
     2161 agcgcccctc gtaggccagc gaaaccttct cgccctcgta gccggtgatc gcggacgggt
     2221 cgtccaggcg gtagatgtcc gtgcgatagc cgggcccgtg gtagccgacc gtcaacaggc
     2281 tgaagttgtg gttgtgcgcg tagtcgtagg catacagcgt gttctcccac gcgtcgtcgg
     2341 ccgcgtaggc gcgcggccgc acccaggtgt tggcgcggat gaagtacttc gggcgcagcg
     2401 ccagcatcat cgtcatcgac gaataggtgc tgaggtcgtt gagctcgtcg tcctgcctga
     2461 agtagtcgtt gagcttgtcg accagcatgc ggcggtccag cgccagcgcc tgcagcatcg
     2521 cggcgccggc aaagatgttg tcctcgtcgt cgggcgcgag gtgctcgtcg cagtaggcga
     2581 tgtactcgtc cagggagatg ggcgcggcgc acgcgtcgcg cgcgaggaat cgggccatgg
     2641 gggtgcctcg ctcagtggat gttcagcttc tcgagcgccc gcgcggcggc gtgggccacg
     2701 tggggatgcg ggtccgcgcg cgcgtcacgc aggcggcgca ggccgcgctc gccatccagc
     2761 gcgcacagcg actggagggc cgtccaccgc aggaaatgga cgtcgtggcc ggccaggcgc
     2821 tcgatggcgt cggcggaacg cgcgcgtgcg gcggcgtcgc catcgtcacc gtgcagcgcg
     2881 acgagcgtgc gcagcatcat ctgcatgcga ctggccgcga ggctgccgga cgaggccatg
     2941 cgcggcaggc cgctcgcctt gtcgtagacc cagaccagcg gcgggccgct gcggcaggtg
     3001 agcttcagta ccagcgtccg ctctgcgcag cggtactccg ggacgtcgcg ggccgcgcgg
     3061 acgacgaacg tgccgccggg cgcgatctcg cggcacgggc gcagggccag gcgctgggtt
     3121 cgatccagcg tgaggatgtc ggccccctcc ggccgctcgt gcacgcacac ggggaagatc
     3181 cccggcccga ggttgccgta gatcgcgtcc gcgaccgggc tgtagatgtg cggcgaggcg
     3241 ccgtggcgcg cgtccatgag cgtggccgtg atggcgaccg cctcggactg cgccagcacc
     3301 atcgtgccgc cggaaaagcc gctcgggacg tggccgaagt cctcgtccag gtggcgcacc
     3361 tcggtgcgca ggagctcggc gatctcgcgc gacgcgacga ggtcgtcgaa gtgcgcccgc
     3421 agcgcgaaga cgccctccgc cgctccggaa tcgatgcgcg cggaagccgc catcaggcgc
     3481 gacaggatcg gcaacgcatc ggggtcgtgc cggaagtggt agttcgacgg cgctgcgccg
     3541 cgacggtcgt tcatggaagc ccctcgggtg gagaaggccg ccgcagcggc cttccgggaa
     3601 cgcgtcagcg cgcgacgatg cgcaggatga tgacgatgat gatggccgcg gtcgggtcgg
     3661 cgaggccgca cggcgcgatg tcggtctcga ggtcgtcgag ctggatcggg ttgttcaagg
     3721 ttctctcctg ggagtgcctg tgagcaggcg acatgggctt gcggccgggc gaggcgccgg
     3781 aaaggcgccc cttgcgcgac cgcgcgaagg ggcggggccg gcgcgcgcgg cgccgtgcgg
     3841 ggcaggagcc ggggacgggc ctggggcccg gtggcattcc tggggaaggc ggcgagcgcc
     3901 gcgagtgccc ggagccgcgc gggcggccgg gtctggtcga ggccggcgtc cacgcggggt
     3961 cgcgatgacg agcggccgat ccaaggttcg cggaaggatc cgcgcatccc gagccgacaa
     4021 ccgaactctg cgtcaactaa ggatttcttt gttttggcta gtttcgggag caacccgacc
     4081 tcgccccatc cctagctcat gggattgcga ccaaacggac gcgatcgaca gccgaacccg
     4141 ggccaccgcc tcccggcggc tccgagcgcg gctgcgcggc gacgcggcgc ctcaggtgcc
     4201 cttggagacc gtgatggtcg ggaacttcgc gctgaagtcc ttggccttgg cggccacgac
     4261 gaccgcgacc tggcgcgcga cggccttgta cagcgccgcg atctcgccgt ccggatcggc
     4321 caccaccgtg ggcgtgccgg agtccgtctg ctcgcggatc gccagcgtca gcggcagcgc
     4381 gccgaggtac ggcaccccgt attgcgccgc catgcgcttg ccgccttccg cgccgaagat
     4441 gtgctcgacg tgcccgcagt tcgtgcagac gtgcacggcc atgttctcga cgatgccgag
     4501 gatgggcacg ccaaccttct cgaacatgtt caggcccttc ctcgcgtcga gcagcgcgat
     4561 gtcctgcggc gtcgtgacga tcaccgcgcc ggtcagcggc acgcgctggc tcagcgtgag
     4621 ctggatgtcg ccggtgccgg gcggcatgtc gacgacgagg tagtcgagct cgccccagtt
     4681 cgtctgccgc agcagctggt cgagcgcctg cgtggccatc gggccgcgcc agacgacggc
     4741 ctgctcggga tcgaccagga acccgatcga catcaccttg acgccgtggt tggcgagcgg
     4801 ctccatcgtc ttgccgtcgg ccgactcggg acggccctcg atgcccatca tcagcggcag
     4861 gctggggccg tagatgtccg cgtccagcag gcccacgcgc gcgccctcgg ccgcgagcgc
     4921 gagcgccagg ttgaccgccg tggtgctctt gccgacgccg cccttgcccg acgacacggc
     4981 gaccacgttg gccacgcccg gcagcagcgc cacgccgcgc tgcacctggt gcgcgacgac
     5041 cttgctcgag accgcgacct cgaccgccgt cacgccggcg acgccgcgcg ccgcggcggc
     5101 cagcgcctcg cgcacggcgg caaactggct cttcgcggga tacccgagct cgacctcgac
     5161 cgcgacgcgc ccgtccgcgt ccgcccgcag cttcttcagc tgccgccccg ccgtgtaggg
     5221 ctggctggtg tggggatcga cgacggcagc gagcgcggcg gcgagcgcgg aatcggagac
     5281 gggcatggga gcggatcggc gggatgcggc ctgcgggccg agacaggcga gcagtctacc
     5341 gcgcggcccc cgaaaccctt cggcgcgcgg cggtccacgg cggcggtcga ggcgccggaa
     5401 cgctcgtgct tgcccgcagt cgcctcaagc gcggcggtgc ggcgccgata tcgcaggaca
     5461 gatcatggaa ctctgccccc tcgtcaaggt ccagcaccgc atcaagctcg ggacgccgct
     5521 gccgttcagc atccgcgacg gccagagcaa gctgctgctc gccaaggacc aggtggtcaa
     5581 ggacgaggaa cagctcgagg ccctgatgga ccggggcgcc tacgtcaatc tcgacgagct
     5641 gcggggcgcg cgcgcggaga tctacaacgc gccgccggag ctgctgccct cgctgtggcg
     5701 caagctcgcc gaccagctct cgcgcacgct cgtgggcatg ccctcgcccc aggccccgca
     5761 ggcgctcgac gaggccgcgc gctacctcgc ggcgctcgcc gagcgcgcgc ccgacgtcgc
     5821 gatcttcctg ctcgtgcggc cggatccgac ctcccccgtc cgctacggcg tgatgcgcag
     5881 cctgcacgcg gccgtcgcgg ccttcctgat cgcgctgcgc ctcgcctggc cgaccgaacg
     5941 ctgcctgacg ctcgtgaagg cggccttcac gatgaacctg ggcatgctgg atctgcagga
     6001 gcgcctgtcc aaccaggaac gcgcgctcaa tccgggccag cgccaggcga tccacgccca
     6061 tccgctgacg agcgtcgcgt cgctgaagga cagcggcgtc gccgacgccg actggctcga
     6121 ggccgtcgcc cagcaccacg agcggcccgg cggcgccggc tatcccgcgg gcctcgcgca
     6181 agtcggcgag atggcctgcg cgttgcgcac cgtcgacgtg ttcaccgcca agctggcggg
     6241 ccgcgccggc cgcgcgccgc tggcgcccac g
//
LOCUS       DASVLN010000912        13174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_263320, whole genome shotgun sequence.
ACCESSION   DASVLN010000912 DASVLN010000000
VERSION     DASVLN010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263320"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VF453_21360"
     CDS             complement(<1..569)
                     /locus_tag="VF453_21360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690269.1"
                     /translation="MTSTSTADALDRLPGLARSQLAVGALFLALGFNYGTWTSRLPAI
                     KAQLGLSTGQVSLVLLAAALGAVFSFPVTAAAVHRFGSRGASLLSAGLLPFTTLALGI
                     APNLACACVVMFAMGIVASTLDVAMNAQGVEVEGAYGRPVMSRLHAVFSLGTMGGALA
                     ASGVLALTPSVPVHFALASALVWLAALLPR"
     gene            complement(851..1081)
                     /locus_tag="VF453_21365"
     CDS             complement(851..1081)
                     /locus_tag="VF453_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690270.1"
                     /translation="MSKQEVKTTVGADGLRYRAKAAGSPFAATGAFGAATMSCFLCGK
                     HRPRAQLKTRKLLGRAQSVCAPSCKEADAQPA"
     gene            complement(1351..3432)
                     /gene="paaZ"
                     /locus_tag="VF453_21370"
     CDS             complement(1351..3432)
                     /gene="paaZ"
                     /locus_tag="VF453_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEX7690271.1"
                     /translation="MTTQLQSYIGGRFVGTRPGAALASAIDGRPVAFAHADELDFGEA
                     LEHARRTGLPALLALDFQQRAARLKALAKFLMERKDQLYAISAHTGATKTDSWIDIEG
                     GAGTLFAYASMGSNELPSSNLIHEGPAVPLGKKGGFAGTHVLVPRRGVAVHINAFNFP
                     VWGALEKFAPTFLAGMPSIVKPATATSYLTEAMVRLCVESGLLPEGSLQLVIGSTGDL
                     LDRVGGQDVVTFTGSADTAAKLRVRPNLIAHSVPFNAEADSLNCAVLADDVTPDDPEF
                     DLFVKEVAREMTVKAGQKCTAIRRAIVPRRHLQAVAERLKARLAKVVVGDPSLEGVTM
                     GALASRDQQRDVAERVAQLARGNTVLLGADGGFAPRGEGTAAGAFFAPTLLLCERPDM
                     ASAAHDVEAFGPVSTLMPYDDFDEALALAERGRGSLVGTLVTHDPARASHAVARLAAL
                     HGRLLVLDRDAAPESTGHGSPLPMLKHGGPGRAGGGEELGGIRAVKHYLQRAAVQGSP
                     TMLAAVTGEYVKGAAVRESPVHPFRHWFEDLRVGDSLLTHRRTVSEADITAFGGISGD
                     FFYMHFDEIAAKASPFGKRIAHGYFVLSAAAGLFVSPGEGPVLANYGLDTLRFVKPVG
                     IGDTIRARLTCKRKIDRNRIGADGRGQGVVAWDVEVTNQDGEVVASYDILTLVAKREA
                     PAVAPQAPLAS"
     gene            complement(3551..4714)
                     /locus_tag="VF453_21375"
     CDS             complement(3551..4714)
                     /locus_tag="VF453_21375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7690272.1"
                     /translation="MTQTSSRFTFRALAAAVLALAAGSALADITVGVTVSATGPAASL
                     GIPEKNTFALLPQTIAGQKIHYVILDDGSDTTKAVSNTRKLITEDKADVIVGSTVTPN
                     SLAMIDVVAEAQVPMISMAASSKIVEPMDAKRRWVFKTPQNDIQMSLAIASHMKDHGV
                     KTVGFIGFSDAYGEGWYGEFSKAAAAKGLTIVANERYARNDSSVTAQVLKLQAAHPDA
                     ILIAGSGTPAALPQITLKERGYAGLIYQTHGVANDDFLRVGGKNVEGTYLPAGPVLVA
                     AQLPAANPVRASALDYVNKYEAAYGKGSVSTFGGHAWDAGHLLMAAIPVALKKAQPGT
                     PEFHAALRDALENVKNVAGAHGIFNMSPTDHLGLDQRARVMVRIEKGAWKLQD"
     gene            complement(4748..5425)
                     /locus_tag="VF453_21380"
     CDS             complement(4748..5425)
                     /locus_tag="VF453_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7690273.1"
                     /translation="MARTRSAGYDDQRDAILASAAELFANQGYVATSMNDIAAACGVS
                     KAGLYHYYRDKRELLFQITVGHVARLEQFVAEVLAEEAAADPETRLRALILRFVREYA
                     DAQHEHRVLTEDVRFLEPDEHAQVTAAQRRVVAAFADTIAAVRPTLARNELDRPLAML
                     LFGMINWMFTWLRPDGSLTHEQMAPLVADLFFGGLGAVRKPRAAARPAVLGEAAENHD
                     PLVHDRP"
     gene            complement(5439..6524)
                     /gene="paaE"
                     /locus_tag="VF453_21385"
     CDS             complement(5439..6524)
                     /gene="paaE"
                     /locus_tag="VF453_21385"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEX7690274.1"
                     /translation="MSDNHFHPLRVRSVRPETDEAMVVSFDVPPELGEHFRFVQGQYL
                     TLRRVIGDEDVRRSYSICAGLDDGELRVGVRRVRGGRFSNWLAENLRAGDTIEVMPPQ
                     GRFFVPVETGAERHYLGVAGGSGITPILSIMKTVLVREPGSRFTLVYGNRRAASTMFK
                     EELDDLKNRYLARVAVHHVFSEERVDSELNQGLLDRAKLAEFLGGLIDVARLDHAFVC
                     GPFQMNDEAEAALLAAGMAPERIHVERFGIPIPGDAAALPAQVVEPGDAPDCELTVIR
                     DGVTHALHFHEADGNLLDCAAKAGINVPYSCKTGVCGTCRAHLDAGEVRMARNYALEK
                     RDLDAGFILTCQSHPTTPEVTISFDHR"
     gene            complement(6536..7066)
                     /gene="paaD"
                     /locus_tag="VF453_21390"
     CDS             complement(6536..7066)
                     /gene="paaD"
                     /locus_tag="VF453_21390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEX7690275.1"
                     /translation="MSATIAASQPRSPRVERAWAVLHGVLDPEVPAISVTDLGIVRDV
                     VAHDDALEVVVTPTYSGCPATEAIAQAIVDAVDAAGLGPARVTQRRAPAWTTDWITPE
                     GREKLRAYGIAPPGACAAPASGKVAPIRLMARNVACPRCGSAQTERLSAFGSTACKAL
                     YRCIACREPFEHFKPI"
     gene            complement(7063..7857)
                     /gene="paaC"
                     /locus_tag="VF453_21395"
     CDS             complement(7063..7857)
                     /gene="paaC"
                     /locus_tag="VF453_21395"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEX7690276.1"
                     /translation="MTAVATASIDFAPTPAQDYLLRIADTCLIHAQRLAEWTGHAPIL
                     EEDIALTNLALDLVGQARALLTHVGAQCGLDEDQLAFLREERAFRNATLVELPGRKGG
                     RDFADCVLRNFVLSAWLNMLWTRLEASSDAEVAAIAGKAVKESRYHVAHAADWVVRLG
                     DGTDESARRMRQALERAWPYLAELFEADAVDAAAEASGLGPSRAALQADWQAIVAPVL
                     ADAQLAAPAGFGAFRSTGSRGVHSEHMGYLLAEMQVLQRSFPGGAW"
     gene            complement(7866..8189)
                     /gene="paaB"
                     /locus_tag="VF453_21400"
     CDS             complement(7866..8189)
                     /gene="paaB"
                     /locus_tag="VF453_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEX7690277.1"
                     /translation="MTVSRTDAANAKAQAHEWPLWEVFVRPKAGLDHKHCGSLHAADP
                     KMALQLAREVYTRRQEGVSVWVVRSDQVVASDPADKAMLFDPMEDKVYRHPTFYELPP
                     EVGHM"
     gene            complement(8216..9217)
                     /gene="paaA"
                     /locus_tag="VF453_21405"
     CDS             complement(8216..9217)
                     /gene="paaA"
                     /locus_tag="VF453_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEX7690278.1"
                     /translation="MYTQAMDTAPKEERAAVRSADEAVAEDRFERALDNDEFIEAKDW
                     MPEHYRKTLVRQISQHAHSEIVGMLPEGNWITRAPTLKRKAILLAKVQDEAGHGLYLY
                     AAGETLGTSRDQMLDALHTGKAKYSSIFNYPTLTWADVGVIGWLVDGAAIMNQIPLCR
                     CSYAPYARAMVRICREESFHQRQGYDALLTMMTKGTDAQRAMVQDATNRWWWPSLMMF
                     GPPDDQSPNSAQSMRWGIKRISNDDLRQKFVDATVEQARILGVTLPDPDLKWNEARGH
                     HDFGAIDWSEFWRVVGGEGPCNKERLAARVDAWNEGEWVREAALAHARKHAVRAQAA"
     gene            complement(9228..10535)
                     /gene="paaK"
                     /locus_tag="VF453_21410"
     CDS             complement(9228..10535)
                     /gene="paaK"
                     /locus_tag="VF453_21410"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEX7690279.1"
                     /translation="MPVHPLRPVELDPIERASRDEIAALQLSRLRATLANVYANVPHY
                     TRAFDEKGVHPDDLKSLADLAKFPFTTKAALRENYPFGMFAVPREQVVRVHASSGTTG
                     KPTVVGYTQADIDHWADLVARSLRAAGVRRGDMVHVAYGYGLFTGGLGAHYGAERLGC
                     TVVPMSGGQTEKQVQLIRDFRPDAIMVTPSYMQVICEEFRRQGLDPREMSVKVGIFGA
                     EPWTEAMRRDISRAADIDAVDIYGLSEVMGPGVASECVETQDGPVIWEDHFYPEIIDP
                     ETGEVLPDGEEGELVFTTLTKQALPVIRYRTRDLTRLLPPTARSMRRIGKIVGRSDDM
                     LIIRGVNLFPTQIEEIVLAHGALGGQYQLVVTREGLLDRVEVRCELLPAHAAANPEAV
                     GHELQARIKTLTGVSTAVVVGQPDSIERTLVGKARRVVDLRTR"
     gene            complement(10550..10996)
                     /gene="paaI"
                     /locus_tag="VF453_21415"
     CDS             complement(10550..10996)
                     /gene="paaI"
                     /locus_tag="VF453_21415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEX7690280.1"
                     /translation="MTQQDAQRIAERVRDHMWATDRASQGMGMRVESIGPGRCTMTMA
                     VREDMLNGYGTCQGGLLAALADSCFAFACNAYDEVTVAAGFDVEIVAPGRLGDVLTAS
                     ATEISKTGRLGVYDVDVRNQRGERVAAFRGRSYTMKGKPVLPPDAR"
     gene            complement(11001..11828)
                     /gene="paaG"
                     /locus_tag="VF453_21420"
     CDS             complement(11001..11828)
                     /gene="paaG"
                     /locus_tag="VF453_21420"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEX7690281.1"
                     /translation="MSPSPELQPLSPADAMILVQQRGAVRMLTLNRPAALNSFTQAMH
                     AELRDALDAAAADPAVRAVLVTGAGRGFCAGQDLNDPAMAPAADGRPDIGAVIERNYK
                     PLVQRVQAMPVPVVCAVNGVAAGAGANFAFGCDIVLAARSASFIQAFSKIGLIPDCGG
                     TWLLPRLAGRARALALALTGDKLSAVDAERFGLVWQVVDDAELLPQALALAERLAAQP
                     TKALVATRQALDAAQRMSMDEALALEARVQGELGRADDFAEGVAAFQQKRAPAFTDR"
     gene            12079..12684
                     /locus_tag="VF453_21425"
     CDS             12079..12684
                     /locus_tag="VF453_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HEX7690282.1"
                     /translation="MPCYEIDGLRPVVDPSAYVHPTAVLIGDVVVGPGAYVGPCASLR
                     GDFGRIVLAAGSNVQDTCVIHGFPDSDTRLEENAHVGHGAVLHGCVVRADALVGMNAV
                     VMDEAEVGPGAFVAASAFVRAGMKIPPRSLAAGVPAKVMRELSDQELAWKLEGTRTYQ
                     RLTERCLATMRECAPLAAEEPGRVRLQAGQVQPLIATRRER"
     gene            complement(12716..13138)
                     /locus_tag="VF453_21430"
                     /pseudo
     CDS             complement(12716..13138)
                     /locus_tag="VF453_21430"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT         1808 a         4672 c         4790 g         1904 t
ORIGIN      
        1 cgcggcagca gtgccgcgag ccacaccagc gccgaggcga gcgcgaagtg caccggcacc
       61 gacggcgtga gcgcgagcac gcccgaggcc gccagcgcgc cgcccatcgt gcccaggctg
      121 aagaccgcat gcagccgcga catcaccggc ctgccgtacg cgccctcgac ctcgacgccc
      181 tgcgcgttca tcgcgacgtc cagcgtgctc gccacgatcc ccatcgcgaa catgaccacg
      241 caggcgcagg caaggttggg tgcgatgccc agcgcgaggg tcgtgaacgg cagcaggccg
      301 gccgacagca ggctcgcgcc gcgcgagccg aagcgatgca cggccgcggc cgtgaccggg
      361 aacgagaaca ccgccccgag cgccgccgcg agcagcacca ggctcacctg ccccgtgctc
      421 aggccgagct gcgccttgat cgcgggcagg cgcgacgtcc acgtgccgta gttgaagccg
      481 agggcgagga acagcgcgcc gacggcgagt tggctgcgcg cgaggccggg caagcggtcg
      541 agtgcatcgg cggtggacgt gctggtcatc ggtggaaagg cggatcacag gacccgcacg
      601 ccgacatggc ccgcagggaa gacggcgagg ctatcaggcc ggacgcctgg gaggcttcag
      661 gcgcgcttca tttctgacgg aagcatcagc gctatggcaa tgagcccgca tgcgcgcgag
      721 cctgaccact tccggagagt tcatggaagg acggacgatg cgttcgtggg cgtgccgctc
      781 aaacgaaacg cgcgccactg gggcgcgcgt cgaagaaaca tcttgcccgg actggccggg
      841 cagtcatgcc tcacgccggt tgggcgtcgg cctccttgca ggacggcgcg cagaccgact
      901 gggcgcggcc gaggagcttg cgcgtcttca gctgggcgcg agggcggtgc ttgccgcaga
      961 gaaagcacga catcgtggcc gcgccgaaag cgccggtggc ggcgaacggc gatccggccg
     1021 ccttggcccg gtaacgcagg ccgtcggctc cgacggtggt cttgacttct tgcttcgaca
     1081 tcgagagatg atccttccgg gattgagcga ggcgggacat gcgtctggcg cagcatgttt
     1141 cgcccgagca ggcaacatta tactgtacaa accctcagcc tggagaatgc gggcgcgtgg
     1201 gggcattccc ggtcgagcaa gccctatgcc aaacggccga gcttgccgac gcgggcgctt
     1261 ccacgtcccc gaaccccgtg cggcaggccc gctcgccagg gttcgcgggc cggccggatc
     1321 ccggaaaggc ggcggcccgg acgtattgtc tcagctcgcg agcggggcct gcggcgcgac
     1381 cgcgggcgcc tcgcgcttgg ccaccagcgt caggatgtcg tagctcgcga cgacctcgcc
     1441 gtcctggttc gtcacctcga cgtcccaggc aacgacgccc tggccgcggc cgtcggcgcc
     1501 gatcctgttg cgatcgatct tgcgcttgca ggtcaggcgg gcgcggatcg tgtcgccgat
     1561 gcccaccggc ttgacgaagc gcagcgtgtc caggccgtag ttcgccagca ccgggccttc
     1621 gccgggcgag acgaacagcc ccgcggccgc cgacagcacg aagtagccgt gcgcgatgcg
     1681 cttgccgaag ggagacgcct ttgcggcaat ctcgtcgaaa tgcatgtaaa agaagtcgcc
     1741 ggagatgccg ccgaaggccg tgatgtcggc ctcgctcacg gtgcggcggt gcgtgagcag
     1801 cgagtcgccg acgcgcaggt cctcgaacca gtggcggaac ggatgcaccg gcgactcgcg
     1861 gacggccgcg cccttgacgt actcgcccgt cacggccgcc agcatcgtcg gcgatccctg
     1921 caccgccgcg cgctgcaggt aatgcttgac ggcgcggatg ccgcccagct cctcgccgcc
     1981 gcccgcgcgg cccgggccgc cgtgcttgag catcggcagc ggcgagccgt ggccggtgga
     2041 ctcgggcgcg gcgtcgcgat cgagcaccag cagccgcccg tgaagcgccg ccaggcgcgc
     2101 gacggcgtgc gacgcgcgcg ccgggtcgtg cgtgaccagc gtgcccacca ggctgccccg
     2161 cccgcgctcg gccagcgcca gcgcctcgtc gaagtcgtcg tagggcatca gcgtgctgac
     2221 cggcccgaag gcctcgacgt cgtgggccgc cgaagccatg tccggacgct cgcacagcag
     2281 cagcgtcggg gcgaagaatg cccccgccgc cgttccctcg ccgcgcggcg cgaagccgcc
     2341 gtcggcgccg agcagcaccg tgttgccccg cgccagctgc gcgacgcgtt cggcgacgtc
     2401 gcgctgctgg tcgcgcgagg cgagcgcgcc catcgtgacg ccctcgaggg acgggtcgcc
     2461 cacgaccacc ttcgcgagac gggccttcag ccgctcggcg accgcctgca ggtggcggcg
     2521 cggcacgatg gcgcgccgga tcgccgtgca cttctggccc gccttgacgg tcatctcgcg
     2581 cgcgacctcc ttgacgaaca ggtcgaactc gggatcgtcc ggcgtcacgt cgtcggcgag
     2641 caccgcgcag ttcagcgaat cggcctcggc gttgaagggc acgctgtgcg cgatcaggtt
     2701 cgggcgcacg cgcagcttcg cggcggtgtc ggccgagccc gtgaaggtca ccacgtcctg
     2761 gccgccgacg cgatcgagca ggtcgcccgt cgagccgatc acgagctgca gcgagccctc
     2821 gggcagcagg ccggactcga cgcacaggcg caccatcgcc tcggtcaggt agctggtggc
     2881 cgtcgccggc ttgacgatgc tgggcatgcc ggcaaggaag gtcggcgcga acttctcgag
     2941 cgcgccccag acggggaagt tgaacgcgtt gatgtgcacc gccaccccgc gccgcggcac
     3001 cagcacgtgc gtgcctgcaa agccgccctt cttgcccagc ggcacggccg ggccctcgtg
     3061 gatcaggttc gacgacggca gctcgttgct gcccatgctc gcgtacgcga acagcgtgcc
     3121 ggcgccgccc tcgatgtcga tccagctgtc cgtcttcgtc gcgccggtgt gggcggaaat
     3181 cgcgtacagc tgatccttgc gctccatcag gaacttcgcg agcgccttga ggcgcgccgc
     3241 gcgctgctgg aagtcgagcg ccagcagcgc cggcaagccg gtgcggcggg cgtgttccag
     3301 cgcctcgccg aagtcgagct cgtcggcatg ggcgaaggcg acgggacggc cgtcgatcgc
     3361 gctggcgagc gccgcgccgg gccgcgtgcc gacgaagcgg ccgccgatgt agctttggag
     3421 ttgggtggtc atcgcaagga gcctttcagt cgccggaccc ctgcccgtac gggtatgcgg
     3481 ggcactggga ccggcgccgg ccgcgcgcgt cgtgacgtgc ggccggcggg ggtcgtcgtc
     3541 gaaggagccg tcagtcctgg agcttccagg cgcccttctc gatcctgacc atcacgcgcg
     3601 cgcgctggtc gaggccgagg tggtcggtcg gcgacatgtt gaagatgccg tgcgcgcccg
     3661 ccacgttctt gacgttctcg agcgcgtcgc gcagcgccgc gtggaattcg ggcgtgccgg
     3721 gctgcgcctt cttgagcgcc accgggatcg cggccatcag caggtgcccg gcgtcccagg
     3781 cgtggccgcc gaaggtcgac acgctgccct tgccgtaggc cgcctcgtac ttgttgacgt
     3841 agtccagcgc cgacgcgcgc acggggttgg ccgcaggcag ctgcgcggcc accagcaccg
     3901 ggccggccgg caggtaggtg ccctcgacgt tcttgccgcc gacgcgcagg aagtcgtcgt
     3961 tggccacgcc gtgcgtctgg tagatcaggc ccgcgtagcc gcgctccttg agcgtgatct
     4021 gcggcagcgc cgcgggcgtg ccggagccgg cgatcaggat cgcgtccgga tgcgcggcct
     4081 gcagcttgag cacctgcgcg gtgaccgagg agtcgttgcg ggcgtagcgc tcgttggcga
     4141 cgatggtcag gcccttggcc gccgcggcct tcgagaactc gccgtaccag ccctcgccgt
     4201 aagcgtccga gaagccgatg aagcccacgg tcttcacgcc gtggtccttc atgtgcgagg
     4261 cgatcgcgag cgacatctgg atgtcgttct gcggcgtctt gaagacccac ctgcgcttgg
     4321 cgtccatcgg ctcgacgatc ttggacgagg ccgccatcga gatcatcggc acctgcgcct
     4381 cggcgacgac gtcgatcatc gccagcgagt tgggcgtcac cgtggagccc acgatcacgt
     4441 cggccttgtc ctcggtgatc agcttgcgcg tgttgctgac ggccttggtc gtgtcggagc
     4501 cgtcgtccag gatcacgtag tggatcttct ggccggcgat cgtctgcggc agcagcgcga
     4561 aggtgttctt ctccgggatt cccagcgacg cggccgggcc ggtggcggac accgtcacgc
     4621 cgaccgtgat gtcggccagc gcgctgccgg cggcgagcgc cagcacggcg gccgcgaggg
     4681 cgcggaacgt gaagcgggag gaggtctggg tcatgggcat cgtctccgtc ttgcgttgaa
     4741 tcggtcttca ggggcggtcg tggaccagcg gatcgtgatt ctccgccgct tcgccgagga
     4801 cggccggacg cgcggccgcg cgcggcttgc gcaccgcgcc gaggccgccg aagaacaggt
     4861 cggccaccag cggcgccatc tgctcgtgcg tcaggctgcc gtcggggcgc agccacgtga
     4921 acatccagtt gatcatgccg aacagcagca tggccagcgg ccggtccagt tcgttgcgcg
     4981 ccagcgtcgg gcgcacggcg gcgatcgtgt cggcgaaggc ggcgaccacg cgccgctgtg
     5041 ccgcggtcac ctgcgcgtgc tcgtcgggct cgagaaagcg cacgtcctcg gtgagcacgc
     5101 gatgctcgtg ctgggcgtcg gcgtactcgc gcacgaagcg caggatcagc gcccgcagcc
     5161 gggtctcggg gtcggccgcg gcctcctcgg ccagcacctc ggcgacgaac tgctccagcc
     5221 gggcgacgtg gcccacggtg atctggaaca gcagctcgcg cttgtcgcgg tagtagtggt
     5281 acaggccggc cttcgagacg ccgcacgccg ccgcgatgtc gttcatcgag gtcgcgacgt
     5341 agccctggtt cgcgaacagc tccgcggcgc tcgcgaggat cgcgtcgcgc tggtcgtcgt
     5401 agccggcgga gcgggttctg gccatcgacc gcggcgcctc agcgatggtc gaaggagatc
     5461 gtcacctcgg gcgtcgtcgg atgcgactgg caggtgagga tgaagccggc gtcgaggtcg
     5521 cgcttctcga gcgcgtagtt gcgcgccatg cgcacctcgc ccgcgtccag gtgcgcccgg
     5581 caggtgccgc agaccccggt cttgcacgag tacggcacgt tgatgcccgc cttggccgcg
     5641 caatcgagca ggttgccgtc ggcctcgtgg aagtgcagcg cgtgcgtgac cccgtcgcgg
     5701 atcacggtga gctcgcagtc gggcgcgtcg ccgggctcga cgacctgggc cggcagcgcg
     5761 gcggcgtcgc ccgggatggg gatgccgaag cgctcgacat ggatgcgctc gggcgccatg
     5821 cccgcggcca gcagcgcggc ctcggcctcg tcgttcatct ggaacggccc gcagacgaag
     5881 gcgtggtcga ggcgagcgac gtcgatcagg ccgcccagga actcggccag cttggcacga
     5941 tccagcaggc cctggttcag ctcggagtcg acgcgttcct cggagaagac gtggtgcacc
     6001 gcgacacgcg cgaggtagcg gttcttgagg tcgtcgagct cctccttgaa catcgtcgag
     6061 gccgcgcggc ggttgccgta gaccagcgtg aagcggctgc cgggctcgcg caccagcacc
     6121 gtcttcatga tggacaggat gggcgtgatg ccgctgccgc ccgcgacgcc gaggtagtgg
     6181 cgctcggcgc ccgtctccac cggcacgaag aagcggccct gcggcggcat cacctcgatc
     6241 gtgtcgccgg cgcgcaggtt ctctgcgagc cagttcgaga agcgcccccc gcgcacgcgc
     6301 cgcacgccca cgcgcagctc gccgtcgtcc agcccggcgc agatcgagta cgagcgccgc
     6361 acgtcctcgt cgccgatcac gcggcgcagc gtgaggtact gcccctgcac gaagcggaag
     6421 tgctcgccca gctccggcgg cacgtcgaac gagacgacca tcgcctcgtc ggtctcgggc
     6481 cgcaccgagc gcacgcgcag cgggtggaag tggttgtcgg acatggcggt cgctctcaga
     6541 tcggcttgaa gtgttcgaag ggttcgcggc aggcgatgca gcggtacagc gccttgcacg
     6601 ccgtcgagcc gaaggccgac agccgctcgg tctgcgcgct gccgcagcgc gggcaggcga
     6661 cgttgcgcgc catcaggcgg atcggcgcga ccttgcccga tgccggcgcg gcacacgcgc
     6721 ccggcggcgc gatgccgtag gcgcgcagct tctcgcggcc ttcgggcgtg atccagtcgg
     6781 tcgtccacgc gggcgcgcgc cgctgcgtga cgcgcgcggg cccgaggccg gccgcgtcca
     6841 ccgcgtcgac gatggcctgc gcgatcgctt ccgtcgcggg gcagcccgag taggtcggcg
     6901 tgaccacgac ctccagcgcg tcgtcgtgcg cgaccacgtc ccgcacgatg ccgaggtccg
     6961 tcacggagat cgccggcact tccgggtcga gcaccccgtg cagcaccgcc cacgcgcgct
     7021 cgacgcgagg cgaccgcggc tgggaggcgg cgatcgtcgc gctcaccacg ccccgcccgg
     7081 gaagctgcgc tgcagcacct gcatctcggc gagcaggtag cccatgtgct cgctgtgcac
     7141 gccgcgcgag ccggtcgagc ggaacgcgcc gaagccggcc ggcgccgcga gctgcgcgtc
     7201 ggcgagcacc ggcgcgacga tcgcctgcca gtccgcctgc agcgccgcgc gcgacgggcc
     7261 cagtccgctc gcctcggcgg cggcgtcgac cgcgtcggcc tcgaacagct ccgcgagata
     7321 gggccacgcg cgctcgagcg cctggcgcat gcggcgcgcc gattcgtccg tgccgtcgcc
     7381 caggcgcacg acccagtcgg ccgcgtgcgc gacgtggtag cgcgactcct tgacggcctt
     7441 gcccgcgatc gcggcgacct cggcgtcgga cgacgcctcg agccgcgtcc acagcatgtt
     7501 cagccaggcc gacagcacga agttgcgcag cacgcagtcg gcgaagtcgc ggccgccctt
     7561 gcggcccggc agctcgacga gcgtcgcgtt gcggaacgcg cgctcctcgc gcaggaaggc
     7621 gagctggtcc tcgtcgaggc cgcactgcgc cccgacgtgc gtgagcagcg cgcgggcctg
     7681 gcccacgagg tccagcgcga ggttggtcag cgcgatgtcc tcctcgagga tgggcgcgtg
     7741 gcccgtccac tcggccaggc gctgggcgtg gatcaggcag gtgtcggcga tgcgcagcag
     7801 gtagtcctgc gcgggcgtcg gcgcgaagtc gatcgaggcg gtggcgacgg cggtcatggc
     7861 gggcctcaca tgtggccgac ttcgggcggc agctcgtaga aggtcggatg gcggtagacc
     7921 ttgtcctcca tcggatcgaa cagcatcgcc ttgtcggccg ggtcggacgc gaccacctgg
     7981 tcggagcgca ccacccacac gctcacgccc tcctggcgcc gcgtgtagac ctcgcgcgcc
     8041 agctgcagcg ccatcttcgg gtcggcggcg tgcaggctgc cgcagtgctt gtggtcgagg
     8101 ccggccttcg ggcgcacgaa cacttcccac agcggccact cgtgagcttg cgccttcgcg
     8161 ttggcggcgt cggtcctgga tacggtcatg gggatcttcc tttcgcggat gcggatcagg
     8221 cggcctgcgc gcgcacggcg tgcttgcggg cgtgggcgag cgcggcctcg cgcacccact
     8281 cgccctcgtt ccaggcgtcg acgcgggcgg ccaggcgttc cttgttgcac gggccctcgc
     8341 cgccgacgac gcgccagaac tcgctccagt cgatcgcgcc gaagtcgtgg tggccgcggg
     8401 cctcgttcca cttcaggtcg ggatcgggca gcgtgacccc gaggatcctc gcctgctcca
     8461 ccgtcgcgtc gacgaacttc tggcgcaggt cgtcgttgct gatgcgcttg atgccccagc
     8521 gcatcgactg cgcgctgttg ggcgactggt cgtccggcgg gccgaacatc atcagcgacg
     8581 gccaccacca gcggttcgtc gcgtcctgca ccatcgcgcg ctgggcgtcg gtgcccttgg
     8641 tcatcatcgt cagcagcgcg tcgtagccct ggcgctggtg gaacgactcc tcgcggcaga
     8701 tgcgcaccat cgcgcgcgcg tagggcgcat acgagcagcg gcacagcggg atctggttca
     8761 tgatggccgc gccgtcgacc agccagccga tcacgccgac gtccgcccag gtgagcgtgg
     8821 ggtagttgaa gatcgagctg tacttggcct tgccggtgtg cagcgcgtcc agcatctggt
     8881 cgcgcgaggt gcccagcgtc tcgcccgcgg cgtagaggta gaggccgtgg ccggcctcgt
     8941 cctgcacctt ggcgagcagg atggccttgc gcttgagcgt cggcgcccgc gtgatccagt
     9001 tgccctcggg cagcatgccg acgatctccg agtgcgcatg ctggctgatc tggcgcacca
     9061 gcgtcttgcg gtagtgctcg ggcatccagt ccttggcctc gatgaactcg tcgttgtcca
     9121 gcgcgcgctc gaagcgatcc tcggcgacgg cctcgtcggc gctgcgcacg gcggcgcgct
     9181 cttccttcgg ggccgtgtcc atggcttgtg tgtacatggc gaactcctta tctcgtcctg
     9241 agatccacca cgcggcgggc cttgcccacc agcgtgcgtt cgatcgaatc cggttgcccc
     9301 acgacgaccg cggtgctcac gcccgtcagc gtcttgatgc gcgcctgcag ctcatggccg
     9361 accgcctcgg ggttggccgc ggcgtgcgcc ggcagcagct cgcagcgcac ctcgacccgg
     9421 tccagcaggc cctcgcgcgt gacgacgagc tggtactgcc cgcccagcgc gccgtgggcg
     9481 agcacgatct cctcgatctg cgtcgggaac aggttgacgc cgcggatgat gagcatgtcg
     9541 tccgagcggc cgacgatctt gccgatgcgg cgcatgctgc gcgcggtggg cggcagcagg
     9601 cgcgtgaggt cccgcgtgcg gtagcggatc accggcagcg cctgcttggt gagcgtcgtg
     9661 aagacgagct cgccctcctc gccgtcgggc agcacctcgc cggtctcggg atcgatgatc
     9721 tcgggataga agtggtcttc ccagatcacc gggccgtcct gggtctcgac gcattcgctc
     9781 gcgacgcccg ggcccatcac ctcgctcagt ccgtagatgt cgacggcgtc gatgtccgcg
     9841 gcccgcgaga tgtcgcgccg catcgcctcc gtccagggct cggccccgaa gatgccgacc
     9901 ttcacgctca tctcgcgcgg atcgagcccc tggcggcgga actcctcgca gatcacctgc
     9961 atgtaggagg gcgtgaccat gatggcgtcg ggccggaagt cgcggatcag ctgcacctgc
    10021 ttctccgtct ggccgccgga catcggcacg acggtgcagc ccaggcgctc ggcgccgtag
    10081 tgcgcgccca gcccgccggt gaacaggccg tagccgtagg ccacgtgcac catgtcgccg
    10141 cggcgcaccc ctgcggcgcg cagcgaacgc gcgacgaggt cggcccagtg gtcgatgtcg
    10201 gcctgcgtgt agccgacgac cgtgggcttg ccggtcgtgc ccgaggacgc gtgcacgcgc
    10261 acgacctgct cgcgcggcac ggcgaacatc ccgaacggat agttctcgcg cagcgcggcc
    10321 ttggtggtga acgggaactt cgccaggtcg gcgagcgact tcaggtcgtc cggatgcacg
    10381 cccttctcgt cgaacgcgcg cgtgtagtgg ggcacgttgg cgtagacgtt ggcgagcgtc
    10441 gcgcgcaggc gcgagagctg cagcgccgcg atctcgtcgc ggctggcgcg ctcgatcggg
    10501 tcgagctcga cggggcggag cggatggacc ggcatcttcg aagaccctct cagcgggcgt
    10561 ccggcggcag caccggcttg cccttcatcg tgtaggagcg gccacggaac gcggccacgc
    10621 gctcgccgcg ctggttgcgg acgtcgacgt cgtacacgcc gaggcggccg gtcttgctga
    10681 tctcggtggc gctcgcggtg aggacgtcgc cgaggcgccc gggcgcgacg atctcgacgt
    10741 cgaagcccgc cgccacggtg acctcgtcgt aggcgttgca ggcgaaggcg aagcacgagt
    10801 cggccagcgc ggcgagcagc ccgccctggc aggtgccgta gccgttgagc atgtcctcgc
    10861 gcaccgccat cgtcatcgtg cagcggccgg ggccgatgga ctcgacgcgc atgcccatgc
    10921 cctgcgacgc gcgatccgtg gcccacatgt ggtcgcgcac gcgctcggcg atgcgctggg
    10981 cgtcctgctg cgtcatgccg tcagcgatcc gtgaacgccg gcgcgcgctt ttgctggaag
    11041 gcggcgacgc cctcggcgaa gtcgtccgcg cggcccagct cgccctgcac gcgcgcctcg
    11101 agcgccagcg cctcgtccat cgacatgcgc tgcgcggcgt cgagcgcctg gcgcgtcgcg
    11161 accagcgcct tcgtcggctg cgcggccagg cgctcggcca gcgccagcgc ctgcggcagc
    11221 agctcggcat cgtcgacgac ctgccagacg agcccgaagc gctcggcgtc gaccgcagac
    11281 agcttgtccc ccgtcagcgc cagcgccagc gcacgcgcgc ggccggccag gcgcggcagc
    11341 agccaggtgc cgccgcaatc ggggatcagg ccgatcttgg agaacgcctg gatgaagctg
    11401 gccgagcgcg cggcgagcac gatgtcgcag ccgaaggcga agttggcgcc tgcgccggcg
    11461 gccacgccgt tgacggcgca caccaccggc accggcatcg cctgcacgcg ttgcacgagc
    11521 ggcttgtagt tgcgctcgat cacggcgccg atgtcgggcc tgccgtcggc ggccggcgcc
    11581 atcgcggggt cgttgagatc ctgccccgcg cagaagccgc ggcccgcgcc ggtcacgagc
    11641 accgcgcgca cggccgggtc ggcggccgcg gcgtcgagcg cgtcgcgcag ctcggcgtgc
    11701 atcgcctggg tgaagctgtt gagcgccgcg ggacgattga gcgtcagcat gcgcaccgcg
    11761 ccgcgctgct gaacgaggat catcgcgtcg gcgggcgaca gcggctgcag ttcgggcgac
    11821 ggactcatgc gtgggcctcg gcggtggaac gggcgtggaa gcgtcggtcg tttgccggcg
    11881 cgggtcgtgg tgtcggggcc cggccgggcg ccgcggcgcc gcccggggtt gtctccccca
    11941 ccggcctccg gcatcgaact cgaccgtggg cgaattcttt gaccgaccgg tcggttaatc
    12001 ttagactcgt cgttgacgca gcgcaagtcc gagcgactcg cacggtcatc gcgccgcacc
    12061 ccaaaccgag gaagtcccat gccctgctac gagatcgacg gcctgcgccc cgtggtcgac
    12121 cccagcgcct acgtgcaccc gaccgcggtg ctgatcggcg acgtcgtcgt cggccccggc
    12181 gcctacgtcg ggccctgcgc gagcctgcgc ggcgacttcg gccgcatcgt gctggcggcc
    12241 ggaagcaacg tgcaggacac ctgcgtgatc cacggctttc ccgactccga cacccggctg
    12301 gaggagaacg cccacgtcgg ccatggcgcc gtgctgcacg gctgcgtcgt gcgcgcggac
    12361 gcgctggtgg gcatgaacgc cgtggtgatg gacgaggccg aggtcgggcc cggcgccttc
    12421 gtcgcggcga gcgcattcgt gcgcgcgggc atgaagatcc cgccgcgctc gctcgccgcg
    12481 ggcgtgccgg ccaaggtgat gcgcgagctg agcgaccagg agctcgcctg gaagctcgag
    12541 ggcacgcgca cctaccagcg gctcaccgag cgctgcctcg cgacgatgcg ggagtgcgcg
    12601 ccgctcgcgg ccgaggagcc ggggcgggtc cggctgcagg cggggcaggt gcagccgctg
    12661 atcgcgacgc gccgggaacg ctgacggcgc ggcgcgcgcc gaagcggtgc cggaactgcg
    12721 cgggcgtgca tccgaccgtg cgctggaacg cgcggtacag gctcgcctcg ctgccgaagc
    12781 cgaggcggtc gacgatcgcc ttgaccggct cgtccgaggc cgcgagcgcc tcgcccacct
    12841 ggcgcagctt cacgcgccgc agccagtcgc ccgccgcgag gccggtgccg gactgcacga
    12901 ggcgcgcgaa gctgcgcacc gacatgccga gccagccggc ggcggcctcc agcgtcagcg
    12961 cctgcgcggg acagcgctgc gcgaacgcga gcgcccgccg cagccgcgcg tcggcgatcg
    13021 cgatcatctc caccggccgg aacaccggca tccactcgcg cggccgcggc agcatcagga
    13081 cgtcctccac gtcgcgccgc gcgtcggacg acagccgcgc ctccagcgcc gcggccatca
    13141 gcggcaggtg gccgttcggg ccgcaggcgg tgcg
//
LOCUS       DASVLN010000913         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_46157, whole genome shotgun sequence.
ACCESSION   DASVLN010000913 DASVLN010000000
VERSION     DASVLN010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46157"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..568
                     /locus_tag="VF453_21435"
     CDS             239..568
                     /locus_tag="VF453_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690283.1"
                     /translation="MANQSPDHASAPDPTRDGQKRVVHDQSLHPAAAPSSHGSAAPET
                     EPPLPQDIDQSAHSQASASATHVDVGLQAHADQTGASRDTDRGPVLDEVYNRTLAPDR
                     GETPPRQ"
     gene            complement(695..1696)
                     /locus_tag="VF453_21440"
     CDS             complement(695..1696)
                     /locus_tag="VF453_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7690284.1"
                     /translation="MTIPRHHDHGACRRARLPAPADDRRESGPGGGKGASAAKAGRLQ
                     DAAGGYPKPPFPRQEQAWPGLASRMEPRPDHGEKSYVGSGRLKDRKALVTGGDSGIGR
                     AAAIAFAREGADVAINYLPQEEQDAQEVVALIREAGRKAVAIPGDLRDAQFCKALVEQ
                     AVKELGGLDILVNNAARQTACDSILELDEQQFDDTMKTNVYALYRVTKAALPHLPRGA
                     AIINTSSVQAFDPSKDLLDYAATKAAILSMTRSLAKQLGDKGIRVNAVAPGPYWTALQ
                     PSGGQKPEKVEKFGEQTPLGRPGQPAEIAPQYVLLASAEASYVTGNVWGSTGGETGP"
BASE COUNT          234 a          718 c          688 g          251 t
ORIGIN      
        1 cgcgctcgcc gccgggctct ttcgcgagca gggcgatgcg cgcgcctact tcgcccgcct
       61 cgattggcgg ctgggcgagc gcgcgcgggc gggcgagcgg gcgttcgccg gggcgatggc
      121 cgaggcctga cggaccggcg accgcccccg catcctcgcg caaggcaagg acggggcgca
      181 ccccgggggc ccggcgcttg cctccaggca tcgacccata gccgaaggag tccgatccat
      241 ggccaaccaa tcccccgacc acgcgtccgc gcccgacccg acgcgcgacg gccagaagcg
      301 cgtcgtccac gaccagagcc tgcacccggc cgcggcgccg tcctcccacg gcagcgctgc
      361 gcccgagacc gagccgccgc tgccgcagga catcgaccag tccgcgcaca gccaggccag
      421 cgccagcgcg acgcacgtcg acgtcgggct gcaggcccac gccgaccaga cgggcgcgtc
      481 gcgcgacacc gaccgcggcc cggtcctcga cgaggtctac aaccgcacgc tcgcgcccga
      541 ccgcggcgag acgccaccgc gccagtgacg cggcgcagcg gacgccgctc gcacggacgt
      601 tgccgaagtc gccccgcctt gcttcctgtt caaggttgcc gcatggtcgg atgacgcggg
      661 ccccgcgggc cggacgagcc cgcggcgcgc cacctcacgg cccggtctcg ccgccggtcg
      721 agccccagac gttgccggtg acgtagctcg cctcggccga ggccagcagc acgtactgcg
      781 gcgcgatctc ggcgggctgg ccggggcggc cgagcggggt ctgctcgccg aacttctcga
      841 ccttctccgg cttctggcct ccgctgggct gcagggccgt ccagtagggc ccgggcgcga
      901 cggcgttcac gcggatgccc ttgtcgccca gctgcttggc cagcgagcgc gtcatcgaca
      961 ggatcgccgc cttcgtcgcg gcatagtcca gcaggtcctt cgagggatcg aaggcctgca
     1021 cggacgatgt gttgatgatg gcggcgccgc gcggcaggtg cggcagggcg gccttcgtca
     1081 cgcggtacag cgcatagacg ttggtcttca tcgtgtcgtc gaactgctgc tcgtcgagct
     1141 cgaggatcga atcgcaggcg gtctggcgcg cggcgttgtt gacgaggatg tcgaggccgc
     1201 cgagctcctt cacggcctgc tcgaccagcg ccttgcagaa ctgcgcgtcg cgcagatcgc
     1261 ccgggatcgc gaccgccttg cggccggcct cgcggatcag cgcgacgacc tcctgcgcgt
     1321 cctgctcctc ctgcggcagg tagttgatcg cgacgtccgc gccctcgcgc gcgaacgcga
     1381 tcgccgcggc gcgcccgatg ccggagtcgc ccccggtcac gagcgccttg cgatccttca
     1441 ggcgacccga cccgacgtag ctcttctcgc catggtcggg cctgggctcc atgcggctcg
     1501 cgaggcccgg ccaggcctgc tcctggcgcg ggaacggcgg cttcgggtag ccgccggccg
     1561 cgtcctgcag ccggccggcc ttcgcggcgc tcgcgccctt gccgccgccc gggccggact
     1621 cccggcggtc gtcggcgggg gcgggcagcc gggcgcggcg gcaggcgccg tggtcgtgat
     1681 ggcgtgggat cgtcatgggg gctccgatgc ggtgcggtgg cgaggcttcg gcaagccgga
     1741 tgccgcgacg gcagtccgcg cgcgacgagg cgagccttgc gcccgggcgc ggccgcaggg
     1801 gccgggatgg gggacacggt atgcgacttg ccggtcggcc ccatgaccat cgaagattcc
     1861 gcatccgcat ccgcatccgc atccgcatcc g
//
LOCUS       DASVLN010000914         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_159480, whole genome shotgun sequence.
ACCESSION   DASVLN010000914 DASVLN010000000
VERSION     DASVLN010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159480"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VF453_21445"
     CDS             <1..1357
                     /locus_tag="VF453_21445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7690285.1"
                     /translation="VCPYYLAQEMVRWADVVVGDYNYWFDLGGLLHGLAQANEWRVGV
                     LVDEAHNLLPRAREMYSATLERPALDAARRHAPPSVAGPLKRLARLWNEIAGRQLEPY
                     AAWDDLPAGLANALQNVNAAIGDLSATQPDAVAPELLSFWFETLHFARLAESFGPHSL
                     FDVTIAGADQRAVTLCLRNLVPASFLRPLFATARTTTLFSATLTPMHFHADLLGLPKD
                     VAWLDVESPFDAAQLRVAVQPLSTRFADRAASVAPIVALMARQFGERPGNYLAFFSSF
                     DYLEQVAAEFRARHPDVPAWVQSRRMDEATRADFLARFAAGGRGIGFAVLGGAFAEAV
                     DLPGDRLIGAFIATLGLPQVNPVNEQMRARLDALFGAGHDYTYLFPGLQKVVQAAGRV
                     IRTTSDEGVVHLIDDRYRRPEVRRLLPAWWRVERPSRIRAAGQPGAAPGTLHAQPEES
                     P"
     gene            1354..1707
                     /locus_tag="VF453_21450"
     CDS             1354..1707
                     /locus_tag="VF453_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690286.1"
                     /translation="MKIETPTWIAEHALVWHKRDGEVTVVARLGKPYRVEHECWACPF
                     ALDGVDGRYPDHQGVSALQALTLAQRVLATRMLQLVDAGERFMQAGDENVWDRAVVEA
                     IFGPGRDLGLVPGRT"
     gene            1704..>2008
                     /locus_tag="VF453_21455"
     CDS             1704..>2008
                     /locus_tag="VF453_21455"
                     /EC_number="4.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550399.1"
                     /note="catalyzes the formation of L-proline from
                     L-ornithine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX7690287.1"
                     /translation="MKHFDAAATARALAWPALIGALERLFVEGCEVPARQVHAVGDAL
                     TSLVMPAWQAGRFYGVKVVNVAAGNAARGLPAVFASYLLFDARTGEPVASIDGSELTA
                     "
BASE COUNT          245 a          746 c          733 g          284 t
ORIGIN      
        1 ggtctgcccg tactacctcg cgcaggagat ggtgcgctgg gcggacgtcg tggtcgggga
       61 ctacaactac tggttcgacc tcggcggcct gctgcacggc ctcgcgcagg ccaacgaatg
      121 gcgcgtgggg gtgctcgtcg acgaggcgca caacctgctg ccgcgcgcac gcgagatgta
      181 ctcggcgacg ctggagcgcc ccgcgctcga cgccgcccgc cgccacgcgc cgccgtccgt
      241 cgccgggccg ctgaagcgcc tcgcgcggct gtggaacgag atcgccggcc gccagctcga
      301 gccgtacgcg gcgtgggacg acctgcccgc cggcctcgcg aacgcgctgc agaacgtcaa
      361 cgccgcgatc ggcgacctgt cggcgacgca gcccgacgcg gtcgcgcccg agctgctgtc
      421 gttctggttc gagacgctgc acttcgcgcg gctggccgaa tcgttcgggc cgcactcgct
      481 gttcgacgtc acgatcgcgg gcgcggacca gcgcgccgtc acgctgtgcc tgcgcaacct
      541 cgtgccggcg tcgttcctgc ggccgctgtt cgcgacggcg cgcacgacga cgctgttctc
      601 ggcgacgctc acgccgatgc acttccacgc cgacctgctc ggcctgccga aggacgtcgc
      661 gtggctcgac gtcgagtcgc ccttcgacgc cgcgcagctg cgcgtggcgg tgcagccgct
      721 gtccacgcgc ttcgccgacc gcgccgcctc ggtcgcgccg atcgtcgccc tgatggcgcg
      781 ccagttcggc gagcggcccg gcaactacct cgcgttcttc agcagcttcg actacctcga
      841 gcaggtcgcc gccgagttcc gcgcccgcca tcccgacgtg ccggcctggg tgcagtcgcg
      901 ccgcatggac gaggccacgc gcgccgactt cctcgcccgc ttcgccgccg gcggccgcgg
      961 catcggcttc gcggtgctgg gcggcgcgtt cgcggaggcc gtcgacctgc cgggcgaccg
     1021 cctgatcggc gcgttcatcg cgacgctcgg gctgccgcag gtcaaccccg tcaacgagca
     1081 gatgcgcgcg cgcctggacg cgctgttcgg cgccggccac gactacacct acctctttcc
     1141 gggcctgcag aaggtcgtgc aggccgcggg gcgggtgatc cgcacgacca gcgacgaggg
     1201 cgtggtgcac ctgatcgacg atcgctaccg ccggccggag gtcaggcggc tgctgccggc
     1261 gtggtggcgg gtcgagcgtc catcccgaat ccgggcggct ggacagcccg gcgcggcgcc
     1321 cggcacactg cacgcgcaac cggaggaatc gccgtgaaga tcgaaacgcc cacctggatc
     1381 gcggagcacg cgctcgtctg gcacaagcgc gacggcgagg tgaccgtggt cgcgcggctg
     1441 ggcaagccct accgcgtcga gcacgaatgc tgggcctgcc cgttcgcgct ggacggcgtc
     1501 gacggccgct accccgacca ccagggcgtg agcgcgctgc aggcgctcac gctcgcgcag
     1561 cgcgtcctcg cgacgcgcat gctgcagctc gtcgacgccg gcgagcgctt catgcaggcc
     1621 ggagacgaga acgtctggga ccgcgcggtc gtcgaggcga tcttcggccc ggggcgcgac
     1681 ctcggcctcg tgccggggcg gacgtgaagc atttcgacgc tgccgcgacc gcgcgcgcgc
     1741 tcgcctggcc cgcgctcatc ggcgcgctcg agcggctgtt cgtcgagggc tgcgaggtgc
     1801 ccgcgcgcca ggtccatgcg gtcggcgacg cgctcacctc gctcgtgatg ccggcgtggc
     1861 aggcggggcg gttctacggc gtgaaggtgg tcaacgtcgc ggcggggaac gcggcgcgcg
     1921 gcttgccggc ggtgttcgcg agctacctgc tgttcgacgc ccgcaccggc gagccggtcg
     1981 cgtcgatcga tggcagcgag ctcacggc
//
LOCUS       DASVLN010000915         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_46276, whole genome shotgun sequence.
ACCESSION   DASVLN010000915 DASVLN010000000
VERSION     DASVLN010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46276"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1401
                     /locus_tag="VF453_21460"
     CDS             136..1401
                     /locus_tag="VF453_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7690288.1"
                     /translation="MTESVRPVSRAARRKAARVGLALAGGGPLGAIWEIGTLCALEEA
                     LVGVDFTRMDGYVGVSAGGFIGAGLANGMTPRQMCTAFIEDEGGADDLIQPAIFVHPA
                     WREYGKRLKALPRLVAQAAWRAALGTGSRLEIVERLGQALPAGFFSNAPIEAHMRQVF
                     SAKGRTDDFRKLGRRLVLVATDLDTGGVAPFGLPGWDDVPISKAVTASAALPGLYPPV
                     RIGGRDYVDGALKKTVHARVLLDEGIDLLFCLNPLVPFDSTYAPVHTVRLGTEPIPRL
                     VEGGLPLVASQALRSLIHSRLELGLKSYRLSHPHVDIALFEPDHRDPELFGANTFSYS
                     ARRQLAEHAYQSARAHLRTRRRALRTMLAAHGIGLDDEALDDRTRRLVTHAPRLQPSW
                     HDAPAGRLGGTLRRLGGILDELESSLAPA"
     gene            complement(1469..>2243)
                     /locus_tag="VF453_21465"
     CDS             complement(1469..>2243)
                     /locus_tag="VF453_21465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7690289.1"
                     /translation="AGTAAALWLNCTLLVGASFSARGLPDGVVATRARRGWRDELVAG
                     MRANWRIPIERGWILANFLSWIFLGPTMTMLVPLKVRALGLSGLWLGLCEAGLSLGLL
                     LGSLGGSQWLVDRFGRFATRVGAAVLQGLALALAGATGTPVLLVACFAVAGTLNATMV
                     LVGKTHRMLARPLAYRSRMIASAVTTTHVAGMIGPALAGVALLHAGVAAVFVVFGVLG
                     GLFALVLAFVPGFRAFMALGHEEVDNYYERMYPRAFVGD"
BASE COUNT          326 a          916 c          740 g          261 t
ORIGIN      
        1 cgcccgcggt ggccctgctt gcgaacgccg gccccgtgcc ggcgttttcg tgtccgccgc
       61 cgcggatccg cccatgcccg cgcgggcatg gcggctgcct ggaaccgact cccacgtccc
      121 gatcgcccgc cgcccatgac cgagtccgtt cgtcccgtct cccgagccgc ccgccgcaag
      181 gccgcgcgcg tcggcctcgc cctggccggc ggcgggccgc tgggtgcgat ctgggagatc
      241 ggcacgctgt gcgcgctcga ggaggccctg gtgggcgtgg acttcacccg catggacggc
      301 tacgtgggcg tctcggccgg cggcttcatc ggcgcgggac tggccaacgg catgacgccg
      361 cgccagatgt gcaccgcctt catcgaggac gagggcgggg cggacgacct gatccagccg
      421 gcgatcttcg tccacccggc ctggcgcgaa tacggcaagc ggctgaaggc gctgccgcgg
      481 ctggtcgcgc aggcggcctg gcgcgcggcg ctgggcacgg ggtcgcggct ggagatcgtc
      541 gaacgcctcg gccaggcgct gccggccggc ttcttcagca acgcgccgat cgaggcgcac
      601 atgcgccagg tcttctcggc gaagggccgc accgacgact tccgcaagct ggggcgccgc
      661 ctcgtgctgg tggccaccga cctggacacc ggcggcgtgg cgccgttcgg cctgccgggc
      721 tgggacgacg tgccgatctc caaggccgtc accgccagcg ccgcgctgcc cgggctctat
      781 ccgcccgtgc gcatcggcgg gcgcgactac gtcgacggcg cgctcaagaa gaccgtgcat
      841 gcgcgcgtcc tgctggacga gggcatcgac ctgctgttct gcctcaaccc gctggtgccc
      901 ttcgactcga cctacgcgcc cgtccacacc gtgcgcctgg gcaccgagcc gatcccgcgc
      961 ctcgtggaag gcggcctgcc gctggtcgcg agccaggcgc tgcgctcgct gatccactcg
     1021 cggctggagc tcggcctgaa gagctaccgc ctcagccacc cgcacgtcga catcgcgctc
     1081 ttcgagcccg accaccgcga ccccgagctg ttcggcgcca acaccttcag ctactcggcc
     1141 cggcgccagc tcgccgagca cgcctaccag tcggcgcgtg cccacctgcg cacccgccgc
     1201 cgcgccctgc gcaccatgct cgcggcacac ggcatcggcc tcgacgacga ggccctcgac
     1261 gaccgcaccc gccgcctcgt cacccacgcc ccgcgcctgc aaccgtcctg gcacgacgcc
     1321 cccgccggcc gcctgggcgg aacgctgcga aggctcggcg gcatcctcga cgaactcgaa
     1381 agctcgctcg cgcccgcgta gggacggcac cgagtccgcg acgacgagcc gcgccggcgt
     1441 ccatccccgc aggcacacgc gtcccgaatc aatccccgac gaacgcccgc ggatacatcc
     1501 gctcgtagta gttgtcgact tcctcgtgcc ccagcgccat gaacgcccgg aaccccggca
     1561 cgaacgccag cacgagcgcg aacagcccgc ccagcacgcc gaagacgacg aacaccgccg
     1621 ccacgcccgc gtggagcaac gccacgcccg cgagcgccgg cccgatcatg ccggccacgt
     1681 gcgtggtggt caccgcgctg gcgatcatgc gcgaccggta ggccagcggc cgcgcgagca
     1741 tgcggtgcgt cttgcccacg agcaccatcg tggcgttgag cgtccccgcc accgcgaagc
     1801 aggccaccag cagcaccggg gtgcccgtcg cgcccgccag cgcgagcgcc agcccctgga
     1861 gaacggccgc tcccacccgc gtcgcgaagc gcccgaagcg atcgaccagc cactgcgagc
     1921 cgcccagcga gccgagcagc agccccagcg acaaccccgc ctcgcacaac cccagccaca
     1981 gccccgacag cccgagcgcc cgcaccttca gcggcaccag catcgtcatc gtcggaccga
     2041 ggaagatcca cgacaggaaa ttggcgagga tccagccacg ctcgatgggg atgcgccagt
     2101 tcgcgcgcat gccggcgacg agctcgtcgc gccatccgcg ccgcgcgcgc gtcgcgacga
     2161 cgccgtccgg cagcccgcgc gcgctgaagg acgcgcccac gagcagcgtg cagttcagcc
     2221 acaacgcggc cgccgtgccc gcg
//
LOCUS       DASVLN010000916         2078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_318433, whole genome shotgun sequence.
ACCESSION   DASVLN010000916 DASVLN010000000
VERSION     DASVLN010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318433"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF453_21470"
     CDS             complement(<1..435)
                     /locus_tag="VF453_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690290.1"
                     /translation="MRLPFLDRRDDLGPAFGRLLLSDAQTLLALMVGQVATPWWIAGA
                     GGAHDLAVYGVFNAALSILAMPLMAPLGDRIPKRRLIRASLLAFAAAAAAVALMASLG
                     AYDLRLVLALGVVPVLAGAAMLPAMSSLVVEIVPPAGLSPAMG"
     gene            complement(506..2011)
                     /locus_tag="VF453_21475"
     CDS             complement(506..2011)
                     /locus_tag="VF453_21475"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEX7690291.1"
                     /translation="MQTDNDARTPGYEALTRTWARLYRLNHLQSLASWDQAANMPPKG
                     NDARALALAEIATIAHGLRTDPALRGHLERARDEPLDDLQRANLREIERDWFRANALP
                     TALVERRMLATSRCEHAWRSQRPANDWAGFAVNLKEVLAIAREEAQHLGDQAGLAPYD
                     AMVNKYEPGMRMQALDRIFGEVGQWLPGLIERVVRKQAAEPVTMPAGPFDRGAQRALC
                     ERVMRVLGFDFDAGRLDVSTHPFSGGVPEDVRLTTRFRDDEFVQSLMGTIHETGHGRY
                     EQNLPRALLGQPVAEARSMGIHESQSLSFEMQVGCHPGFIAHIAPLVVEAFGAQPAFE
                     PGNLQRALTRVQPGLIRVDADEVTYPAHVILRYEIERGLFDGSLEVDDIPALWDEKMA
                     ALLGLDTRGNFRDGPMQDVHWAAGEFGYFPCYSLGAMYAAQWFAAMRRTLPTLDEDIA
                     AGRLGPVFGWLRENIWQQGCRWTTDELAVRASGEVLNPAHFKAHLERRYLG"
BASE COUNT          270 a          755 c          785 g          268 t
ORIGIN      
        1 gcccatcgcc ggcgacagac ccgccggcgg cacgatctcg acgaccaggc tgctcatggc
       61 gggcagcatc gccgcgccgg ccagcaccgg caccacgccc agcgcgagca cgagccgcag
      121 gtcgtaggcg cccaggctcg ccatcagcgc gaccgccgcc gcggcggccg cgaacgcgag
      181 cagcgacgcg cggatcagcc gccgcttcgg gatgcggtcg cccagcggcg ccatcagcgg
      241 catcgcaagg atggacagcg ccgcgttgaa gacgccgtag accgccaggt cgtgcgcgcc
      301 gccggcgccg gcgatccacc agggcgtcgc gacctggccc accatcagcg cgagcagcgt
      361 ctgcgcgtcg ctcaggagca ggcgcccgaa cgcggggccg aggtcgtcgc ggcgatcgag
      421 gaagggcagg cgcatggcgc tgcgaggata gccgcgcacg gccgcgccgg cgggacgatc
      481 ccgtggcgcg gacgcggacg cggcctcagc cgaggtagcg ccgttcgagg tgcgccttga
      541 agtgcgccgg gttcagcacc tcgcccgagg cgcgcaccgc gagctcgtcg gtcgtccatc
      601 tgcagccctg ctgccagatg ttctcgcgca gccagccgaa gaccgggccc aggcggccgg
      661 cggcgatgtc ctcgtcgagc gtcggcagcg tccgccgcat cgccgcgaac cactgcgcgg
      721 cgtacatcgc gcccagcgag tagcacggga agtagccgaa ctcgccggcg gcccagtgga
      781 cgtcctgcat cggcccgtcg cggaagttgc cgcgcgtgtc caggccgagc agcgcggcca
      841 tcttctcgtc ccacagcgcg gggatgtcgt cgacctcgag cgagccgtcg aacaggccgc
      901 gctcgatctc gtagcgcagg atcacgtgcg cggggtaggt gacctcgtcg gcgtcgacgc
      961 ggatcaggcc cggctgcacg cgcgtcagcg cgcgctgcag gttgccgggc tcgaacgccg
     1021 gctgcgcgcc gaaggcctcg acgacgagcg gcgcgatgtg cgcgatgaag ccgggatggc
     1081 agccgacctg catctcgaac gacaggctct ggctctcgtg gatgcccatc gagcgcgcct
     1141 cggccaccgg ctggccgagc agcgcgcgcg gcaggttctg ctcgtagcgg ccgtggcccg
     1201 tctcgtggat ggtgcccatc aggctctgga cgaactcgtc gtcgcggaag cgcgtcgtca
     1261 ggcgcacgtc ctcgggcacg ccacccgaga acggatgcgt gctgacgtcg agccggccgg
     1321 cgtcgaagtc gaagccgagc acccgcatca cgcgctcgca cagggcgcgc tgggcgccgc
     1381 ggtcgaacgg gccggcgggc atcgtcaccg gctcggcggc ctgcttgcgc acgacgcgct
     1441 cgatcagccc cggcagccac tgcccgacct cgccgaagat gcggtcgagc gcctgcatgc
     1501 gcatgccggg ctcgtacttg ttgaccatcg cgtcgtaggg cgcgagcccc gcctggtcgc
     1561 ccaggtgctg cgcctcctcg cgcgcgatcg ccagcacctc cttgaggttg accgcgaagc
     1621 ccgcccagtc gttggccggg cgctggctgc gccaggcgtg ctcgcagcgc gaggtcgcga
     1681 gcatgcggcg ctcgaccagc gcggtcggca gcgcgttggc gcggaaccag tcgcgctcga
     1741 tctcgcgcag gttggcgcgc tgcaggtcgt cgagcggctc gtcgcgggcg cgctcgaggt
     1801 ggccgcgcag cgcggggtcg gtgcgcaggc cgtgggcgat cgtggcgatc tcggccaggg
     1861 ccagggcgcg cgcgtcgttg cccttgggcg gcatgttggc ggcctggtcc cagctcgcca
     1921 ggctctggag gtggttgagc cggtacaggc gcgcccacgt gcgggtgagg gcctcgtagc
     1981 ccggcgtgcg ggcgtcgttg tcggtctgca tgtcgtccct gaggttgccg ccgcgctcgg
     2041 cgcccgccgc gccggcccgc gggcgaggcg catccggt
//
LOCUS       DASVLN010000917        10649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_424567, whole genome shotgun sequence.
ACCESSION   DASVLN010000917 DASVLN010000000
VERSION     DASVLN010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10649
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424567"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VF453_21480"
     CDS             <1..205
                     /locus_tag="VF453_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="desaturase"
                     /protein_id="HEX7690292.1"
                     /translation="EAAPVECIAARADRRATFRCTAGVVRPGMAVAPGLLAAGDYVDG
                     PYPATLEAAVRAGEAAVEASPSR"
     gene            328..1101
                     /locus_tag="VF453_21485"
     CDS             328..1101
                     /locus_tag="VF453_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7690293.1"
                     /translation="MSKSDGSGPSIQVLARAFALLDVLAAHTDPVPLKELSARTGLHP
                     STAHRILNDLTEGRFVERPEAGSYRLGMRLLELGNLVKARLDVRDAAIGPMRELHKLT
                     HQPVNLSVRQGDEIVYIERTYSERSGMQVVRAVGGRAPLHLTSVGKLFLAHDEPQRVR
                     AYATRTGLTGHTRNSLTELPLLERELEKVRRTAIARDDEELELGVRCMAAGIYDDQGR
                     LVAGLSISAPADRLEETWVDRVKETAARISAALGHGSGA"
     gene            complement(1188..2270)
                     /gene="pbpG"
                     /locus_tag="VF453_21490"
     CDS             complement(1188..2270)
                     /gene="pbpG"
                     /locus_tag="VF453_21490"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HEX7690294.1"
                     /translation="MQRLSGFTDMRVIKQISRWAVAVAIGSALALPALDAQAQPVKHH
                     RKTAASAHAPARAHAHARSSSRRVVTSVTTTTRTTARVEPAAASVGTLAGLHNTEDPL
                     YLRSGAALVLDQDTHEVLYSKNPEAVLPIASLSKLMTAMVVSEAHQPLDEVLTITDDD
                     IDTEKNTRSRLKPGTQLTRGVMLHLALMASENRAANALGRNYPGGLQAFVAAMNAKAK
                     ELGMTDTHYVEPTGLSSQNHSTARDLAVLVNAAHQVPLLREYSTTPSLDVELGRRQVQ
                     FHTTDRLVASPAWDIGLQKTGFINEAGQCLVMQAQIAGRKLIMVLLDSAGKYSRIADA
                     ERIRKWVMSYHNAPATTTARATESQG"
     gene            complement(2748..4445)
                     /gene="leuA"
                     /locus_tag="VF453_21495"
     CDS             complement(2748..4445)
                     /gene="leuA"
                     /locus_tag="VF453_21495"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX7690295.1"
                     /translation="MLKNPAAKYRPVAPIRLADRTWPDKVLAKAPVWCSSDLRDGNQA
                     LIEPMNLERKLRMFETLVRIGFKEIEVGFPSASQTDFDFVRKLIEDGLIPDDVQIQVL
                     TPAREHLIRRTFESVVGAKQVIMHLYNATAPIMRKVVLGVDVEGVIELAASHARLIRD
                     LAEKQPETSWRYEYSPEMFSDTELDVAKRVVDAVTAVWEPTPERTCIVNLPTTVEHST
                     PNIFADMMEWMHRHLERRESIVLSVHPHNDRGTGTATGELALMAGADRVEGCLFGNGE
                     RTGNVDIVNIALNLYTQGVDPELDFSDIDEIRRTVEYCNQLPVHPRHPYAGDLVYTSF
                     SGSHQDAIKKAFAARKEGDVWEMPYLPVDPKDLGRSYEAVIRVNSQSGKGGISYLLES
                     EYGLELPRRLQIDFSQVVQAVMDAQGKEVSAADIWQIFRREYRLGEDAPTLAQLSQSD
                     AGDGQVRLAAEVGVQETRIRIEGQGNGPVDAFCDGLSRAVGAQVKVLDYHEHSIGAGA
                     QARAAAYLSVQVGEAPAVFGVGIDANIVHASLLAIVSAVARATKGRVSVAGAEAAAA"
     gene            complement(4726..5562)
                     /gene="pssA"
                     /locus_tag="VF453_21500"
     CDS             complement(4726..5562)
                     /gene="pssA"
                     /locus_tag="VF453_21500"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEX7690296.1"
                     /translation="MNDVAPIPDEDDDDAPVRRPRRKGVYILPNLFTLAALFGGFYAV
                     VMAMNGRYEQACYGVFCAMVLDSLDGRVARMTGTQSAFGAEMDSLSDMVSFGAAPALI
                     VYEWALKGLGKPGWCVAFLYCACAALRLARFNTNLAVVDKRYFQGLPSPAAAALVVGF
                     IWVVVDQLGGGYDVNGDPLLSWAAAAVAFYAGLTMVVNVPFYSFKDLSLKRSVPFVVI
                     VLIALAIGIINLYPAAVIFAIFCAYGISGYLVYAYKRAKGRPVSVIALSTDEPDEVGL
                     HR"
     gene            complement(5866..6882)
                     /gene="ilvC"
                     /locus_tag="VF453_21505"
     CDS             complement(5866..6882)
                     /gene="ilvC"
                     /locus_tag="VF453_21505"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX7690297.1"
                     /translation="MKVYYDKDTDLSLIKGKNVAIIGYGSQGHAHAQNLNDSGVKVTV
                     GLRKGGASWSKVEKAGLKVAEVADAVKAADVVMILLPDENIAEVYKNDVEPHIREGAS
                     LAFAHGFNVHYGQVAPRADLDVWMVAPKAPGHTVRNTYTQGGGVPHLVAVYQDKTGKA
                     RDLALSYAAANGGGKAGIIETSFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGY
                     APEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEYGEYVTGPRVVTDETKKAMKQ
                     ALKDIQTGEYAKSFILENRAGAPTLLSRRRLTAEHPIEVVGEKLRAMMPWIKANKLVD
                     KSRN"
     gene            complement(7092..7583)
                     /gene="ilvN"
                     /locus_tag="VF453_21510"
     CDS             complement(7092..7583)
                     /gene="ilvN"
                     /locus_tag="VF453_21510"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX7690298.1"
                     /translation="MKHIIAVLLENEPGALSRVVALFSARGYNIESLTVATTEDPSLS
                     RMTVVTSGSDEVIEQITKHLNRLIDVVKVVDLTEGQFTERELMLIKVRAVGKEREEMK
                     RMADIFRGHIVDVTEKSYTIELTGDTSKLDAFITAIDRTAILETVRTGTSGIGRGERI
                     LRA"
     gene            complement(7760..9556)
                     /locus_tag="VF453_21515"
     CDS             complement(7760..9556)
                     /locus_tag="VF453_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HEX7690299.1"
                     /translation="MDMSAPDGKRQASGSPHPISSPTTAPAASDPNGSEILVRCLQAE
                     NVKFVWGYPGGAVLYIYDALYKQDAIQHVLVRHEQGAVHAADGYARATGEVGVALVTS
                     GPGVTNAVTGIATAYMDSIPMVIITGQVPTPAIGLDAFQECDTVGITRPIVKHNFLVK
                     DVRELASTIKKAFHIARTGRPGPVVVDIPKDVSQAVAPYHYPEHVEMRSYNPVKKGHG
                     GQIRKAVQLLLNARRPYIYSGGGVVLGNAAAELRELADLLGYPVTNTLMGLGAFPATD
                     PKFLGMLGMHGTFEANMAMQNCDVLLAIGARFDDRVIGNPRHFASVERKIVHVDIDPS
                     SISKRVKVDIPIVGDVKDVLQELIAQIREGQARPDTAVVSQWWSQINEWRRKDCLAYK
                     RSPDVIKPQQVVEALWDLTRGRDCYVTSDVGQHQMWAAQYFRFDEPRRWINSGGLGTM
                     GVGLPYAMGIKLARPQSDVFCITGEGSIQMCIQELSTCQQYNTPVKIISLNNRYLGMV
                     RQWQQLEYSGRYSHSYMDALPDFVKLAEAYGHVGLRISKPEDVEPALREAIALKDRTV
                     FIDVQTDPTENVWPMVKAGKGITEMLLGSEDL"
     gene            9924..10490
                     /locus_tag="VF453_21520"
     CDS             9924..10490
                     /locus_tag="VF453_21520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7690300.1"
                     /translation="MATDKELSDFLKSVEKRAFKRTAFTVRDDDAALDIVQDSMIRLA
                     EKYVDRPAAELPLLFQRILSNATMDWFRRQKVRNAVVNNFSDFEGNDDDGDFDILEML
                     DSVEGTLGTESASDAVSRDQILLLIEGEIGELPARQREAFLLRYWEELDVAETASVMG
                     CSEGSVKTHCSRAVHALAKALKAKGIAP"
     gene            10487..>10649
                     /locus_tag="VF453_21525"
     CDS             10487..>10649
                     /locus_tag="VF453_21525"
                     /inference="COORDINATES: protein motif:HMM:NF023699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEX7690301.1"
                     /translation="MTRNTHQTFTHAPDRDGLEARFGVRVAAVLSERANATAPDISER
                     LRFAREQALA"
BASE COUNT         1626 a         3611 c         3679 g         1733 t
ORIGIN      
        1 cgaggcagcg cccgtggagt gcatcgccgc ccgcgccgat cgccgcgcca ccttccgctg
       61 caccgcaggc gtcgtgcggc cgggaatggc ggtggcgccg gggttgcttg cggccgggga
      121 ctacgtcgac gggccctacc ccgcgacgct ggaagccgcc gtccgcgccg gcgaggccgc
      181 ggtcgaggcc tcgccctcgc gctgaggcgc ggcggcgtcg cgccttcacg actcgcggac
      241 gcgctggaga gacgattcca ggcgccgccg gacgaaatcg gttttcacca tgcaagaatg
      301 tcgagtccac agcctgaggc cgaccccatg tccaagtcag acggctccgg cccgtccatc
      361 caggtgctcg cgcgggcctt cgcgctgctc gacgtcctcg ccgcgcacac cgatccggtg
      421 ccgctgaagg agctcagcgc ccgcaccggc ctgcatccct cgacggcgca ccgcatcctc
      481 aacgacctca ccgaaggccg cttcgtcgag cgtcccgagg ccggcagcta ccggctcggg
      541 atgcggctgc tcgagctggg caacctcgtc aaggcgcggc tggacgtgcg cgacgccgcc
      601 atcggtccga tgcgcgagct ccacaagctc acccaccagc cggtcaacct gtcggtgcgc
      661 cagggcgacg agatcgtcta catcgagcgc acctacagcg aacgttccgg catgcaggtc
      721 gtgcgcgccg tcggcggccg cgcgccgctg cacctgacct cggtgggcaa gcttttcctg
      781 gcccacgacg aaccccagcg cgtgcgcgcc tacgcgacgc gcaccggcct caccggccac
      841 acccgcaaca gcctgaccga gctgccgctg ctcgagcgcg aactggagaa ggtgcgccgc
      901 acggccatcg cgcgcgacga cgaggagctc gagctgggcg tgcgctgcat ggccgccggc
      961 atctacgacg accagggacg cctggtggcc gggttgtcga tctccgcccc cgccgaccgg
     1021 ctcgaggaga cctgggtcga ccgcgtcaag gagaccgccg cgcgcatctc cgcggcgctg
     1081 ggccacggca gcggcgcctg atcgtcggcg gcggcgcctg ccgcacgcat gaaaaaaggc
     1141 cggagcgcga gcgtccggcc ttcgtcgttc cagcggttcg cggttactca gccctgcgat
     1201 tcggtggcgc gcgccgtcgt cgtcgccggc gcgttgtgat agctcatgac ccacttgcgg
     1261 atgcgctcgg catcggcgat gcgcgagtac ttcccggccg agtcgagcag caccatgatc
     1321 aacttgcggc cggcgatctg ggcctgcatc acgaggcact ggccggcctc gttgatgaag
     1381 ccggtcttct gcaggccgat gtcccaggcc gggctcgcga cgaggcgatc ggtggtgtgg
     1441 aactgcacct ggcgacggcc cagctcgacg tccagcgacg gcgtcgtcga atactcgcgc
     1501 agcagcggca cctggtgggc ggcgttgacc agcaccgcga ggtcgcgcgc ggtcgaatgg
     1561 ttctggctcg acagcccggt cggctcgacg tagtgcgtgt ccgtcatgcc gagctccttc
     1621 gccttcgcgt tcatcgcggc gacgaaggcc tgcaggccgc ccgggtagtt ccggcccagc
     1681 gcgttggcgg cgcggttctc cgacgccatc agcgcgaggt gcagcatgac gccgcgcgtg
     1741 agctgcgtgc cgggcttcag gcgcgagcgc gtgttcttct cggtgtcgat gtcgtcgtcg
     1801 gtgatcgtca gcacctcgtc gaggggctgg tgcgcctcgc tgaccaccat ggccgtcatc
     1861 agtttgctga gcgacgcgat cggcaggacg gcctcgggat tcttgctgta gagcacctcg
     1921 tgcgtgtcct ggtcgagcac cagggccgcg cccgagcgca ggtagagcgg atcctcggtg
     1981 ttgtgcaggc ctgccagcgt gccgaccgac gcggccgcgg gctcgacgcg cgcggtcgtc
     2041 cgcgtggtcg tggtgacgct cgtgaccacg cggcggctcg agctcctcgc gtgcgcatgc
     2101 gcgcgcgccg gcgcgtgggc cgaggcggcg gtcttgcgat ggtgcttgac gggctgggcc
     2161 tgggcgtcca gcgccggcaa cgccagcgca ctgccgatcg cgaccgccac cgcccagcgc
     2221 gaaatctgct tgatcacccg catgtcagta aaaccggaaa gccgttgcat caccaattgt
     2281 ttcagtctaa ggcaaaggaa aaagccacgc aagatcaaaa acttgcaagc ctttcctcaa
     2341 ccagagccgc cagatctgcc gatgccactg ccgagtagcc acgacggcgc ccaccggcgg
     2401 acccgggaag gctgattaac gcggactgca ggcagccggc tgacgccgga tcccccaaac
     2461 gggggtgtct ttacacgcag gaggccccga gcctgccgcg cgatgggccc gaggcaacgc
     2521 ctgattcgtg aattctggca agccgcccgc attgcgatct cgcgataagc accggcgcgg
     2581 ccgccatttc gtgacttaag gcacgctgcg cggccgtgcg cggcggagga cgccgcagca
     2641 ggcatcgtgc ccgaatgcgc tgcggcctcc gcgccggtga ggcggggaag ccgcggcccg
     2701 aaccggctcg tgaccggcgc ggggcgatga cggggtcgcc ggcgggatca ggccgcggca
     2761 gcctccgcgc cggcgacgct cacgcgtccc ttcgtggcac gtgcgacggc cgacacgatc
     2821 gccagcagcg aggcgtgcac gatgttggcg tcgatgccga cgccgaacac ggccggcgcc
     2881 tcgccgacct gcacggacag gtaggccgcg gcgcgcgcct gcgcgccggc gccgatcgag
     2941 tgctcgtggt agtccagcac cttcacctgc gccccgacgg cgcgcgacag gccgtcgcag
     3001 aacgcgtcga ccggtccgtt gccctggccc tcgatgcgaa ttcgcgtctc ctgcacgccc
     3061 acctcggcgg ccaggcgcac ctggccgtcg ccagcgtccg actgcgacag ctgggcgagc
     3121 gtgggggcgt cctcgcccag gcggtactcg cggcggaaga tctgccagat gtcggccgcg
     3181 ctgacctcct tgccctgcgc gtccatcacc gcctggacga cctggctgaa gtcgatctgc
     3241 aggcggcgcg ggagctccag cccgtactcc gactccagca ggtaggaaat cccgcccttg
     3301 cccgactggc tgttgacgcg gatgacggcc tcgtagctgc ggccaagatc cttcgggtcg
     3361 accggcaaat agggcatctc ccagacgtcg ccctccttgc gggcggcgaa ggccttcttg
     3421 atcgcgtcct ggtgcgagcc ggagaacgag gtgtagacga ggtcgcccgc gtacgggtgg
     3481 cgcgggtgca ccggcagctg gttgcagtat tcgacggtgc ggcggatctc gtcgatgtcc
     3541 gagaagtcca gctcggggtc gacgccctgc gtgtacaggt tcagcgcgat gttgacgatg
     3601 tcgacgttgc ccgtgcgctc gccgttgccg aagaggcaac cctcgacgcg atcggcaccg
     3661 gccatcagcg cgagctcgcc ggtggcggta ccggtgccgc ggtcgttgtg cgggtgcacc
     3721 gacagcacga tggactcgcg gcgctcgagg tggcggtgca tccactccat catgtcggcg
     3781 aagatgttgg gcgtggaatg ctccaccgtc gtcggcaggt tgacgatgca ggtgcgctcg
     3841 ggcgtcggct cccagaccgc cgtcaccgcg tcgacgacgc gcttggcgac gtcgagctcg
     3901 gtgtcggaga acatctccgg cgaatactcg tagcgccacg aagtctccgg ctgcttctcg
     3961 gcgagatccc ggatcaggcg cgcgtggctc gcggcgagct cgatgacgcc ctcgacgtcc
     4021 acgccgagca cgaccttgcg catgatcggc gcggtcgcgt tgtacaggtg catgatgacc
     4081 tgcttcgcgc cgaccaccga ctcgaaggtg cggcggatca ggtgctcgcg cgcgggcgtc
     4141 agcacctgga tctggacgtc gtcggggatc aggccgtcct cgatgagctt gcgcacgaag
     4201 tcgaagtcgg tctgcgaggc ggacggaaag ccgacctcga tctccttgaa gccgatgcgc
     4261 accagcgtct cgaacatgcg cagcttgcgc tcgaggttca tcggctcgat cagggcctgg
     4321 ttgccgtcgc gcaggtcgga gctgcaccac accggggcct tggccagcac cttgtccggc
     4381 caggtgcggt ccgccaggcg gatcggcgcg acgggacggt acttggcggc gggattcttc
     4441 agcatggtca tggggtcagc tcctgtcggg gtgtcgagtc gatcgcaacg ccgctccaaa
     4501 gacgaacggc ccgcagcgtt gcgctggcgg gccgttgatc ttggggaaac tgggatctct
     4561 gggatctgga atgtgttcga tcaatgcgcc cgcgctacgc cgctaggagt agggaaatga
     4621 gcgcaatgcg ggacgacatg ccgacgaatg tagcacggcc ttcccggcat gtgcacgtga
     4681 aatgttcgcg gttcacgaag cccttcttcg cgcctcggat tttcgtcagc ggtgcagccc
     4741 gacctcgtcg ggctcgtcgg tcgacagggc gatcacgctg accggccggc ccttggcacg
     4801 cttgtacgcg tagacgaggt agcccgagat gccgtacgcg cagaagatcg cgaagatcac
     4861 cgccgccgga tagaggttga tgatgccgat ggccagcgcg atcaggacga tgacgacgaa
     4921 gggcaccgag cgcttcaggc tcaggtcctt gaagctgtag aacggcacgt tgaccaccat
     4981 cgtcagcccc gcgtagaacg cgaccgcggc cgccgcccag gacagcagcg gatcgccgtt
     5041 gacgtcgtag ccgccgccca gctggtcgac cacgacccag atgaagccga ccaccagcgc
     5101 cgccgcggcc gggctcggca agccctggaa gtagcgcttg tcgacgacgg ccaggttcgt
     5161 gttgaagcgc gccagccgca gcgccgcgca ggcgcagtag aggaaggcga cgcaccagcc
     5221 cggcttgccc aggcccttga gcgcccactc gtacacgatg agcgccggcg ccgcgccgaa
     5281 gctgaccatg tccgacaggc tgtccatctc ggcgccgaac gcgctctgcg tgccggtcat
     5341 gcgggcgacg cggccgtcca ggctgtcgag caccatcgcg cagaagacgc cgtagcaggc
     5401 ctgctcgtag cgcccgttca tcgccatcac gacggcgtag aagccgccga acagcgccgc
     5461 gagcgtgaac aggttgggca ggatgtagac gcccttgcgc cgcggccggc gcaccggcgc
     5521 gtcgtcgtcg tcctcgtccg ggatcggcgc gacgtcgttc atcgagggac agcggtgaaa
     5581 ttcatggtgc gcgaggatac cccagcagcc cggccaccag cgaaaaacgg gcgaccgaag
     5641 ccgcccgtct tcccgagtgg acgcgagcga agcgacgcgc cccgaccgcg tccggcgctg
     5701 ccgtcccgag cgaggcacga ggaagcggca gatccggctc ccccggtctg ctcgcctgcc
     5761 ggccgcaagc ggccggcgtt cgccaggcac agtccgtcca ctggacggcc tgtgtccggg
     5821 ctcaccccct cggggggcag ggagcgccag cgaccgtggg gctcgtcagt tcctggactt
     5881 gtcgaccagc ttgttcgcct tgatccaggg catcatcgcg cgcagcttct cgccgacgac
     5941 ctcgatcgga tgctcggcgg tcaggcggcg gcgcgagagc agcgtcggcg cgccggcgcg
     6001 gttctcgagg atgaagctct tggcgtactc gcccgtctgg atgtccttga gcgcctgctt
     6061 catcgccttc ttcgtctcgt ccgtgacgac gcgcgggccg gtcacgtact cgccgtactc
     6121 ggcgttgttc gagatcgagt agttcatgtt ggcgatgccg ccctcgtaga tgaggtcgac
     6181 gatcagcttg agctcgtgca ggcactcgaa gtaggccatc tcgggcgcgt agccggcctc
     6241 gaccagcgtc tcgaagcccg ccttgatcag ctccaccgtg ccgccgcaca gcacggcctg
     6301 ctcgccgaac aggtcggttt cggtctcctc gcggaagctg gtctcgatga tgccggcctt
     6361 gccgccgccg ttggccgcgg cgtagctcag cgcgaggtcg cgcgccttgc cggtcttgtc
     6421 ctggtacacc gccaccagat gcggcacgcc gccaccctgg gtgtaggtgt tgcgcacggt
     6481 gtggccaggc gccttgggcg cgaccatcca gacgtcgagg tccgcgcgcg gcgcgacctg
     6541 gccgtaatgg acgttgaagc cgtgcgcgaa cgcgagcgag gcgccttcgc ggatgtgcgg
     6601 ctcgacgtcg ttcttgtaga cctcggcgat gttctcgtcg ggcagcagga tcatgacgac
     6661 gtcggccgcc ttgaccgcgt cggccacctc ggccaccttg aggccggcct tctcgacctt
     6721 gctccaggac gcgccgccct tgcgcaggcc gacggtgacc ttgacgccgc tgtcgttgag
     6781 gttctgcgca tgggcgtggc cctgggagcc gtagccgatg atcgcgacat tcttgccctt
     6841 gatgagggaa agatcggtgt ccttgtcgta gtagaccttc atggttcgct ctgctttcgt
     6901 cttcaggaaa aaagggtgga tcaaaacgcc ccggcggcga gcgcgcggcc gctccggagc
     6961 gagccgccga gcccccgcgg ggggcggtga gcgcagcgag caggggtagt caatagcccg
     7021 cggccgctcc ggagcgagcc gccgagcccc cgcggggggc ggtgagcgca gcgagcgggg
     7081 gtcgtcatta ctcaggcgcg gaggatgcgt tcgccgcggc cgatgccgct ggtgccggtg
     7141 cgcacggtct cgaggatggc cgtgcggtcg atcgcggtga tgaaggcgtc gagcttggac
     7201 gtgtcgccgg tgagctcgat cgtgtagctc ttctcggtga cgtcgacgat gtggccccgg
     7261 aagatgtctg ccatgcgctt catctcctcg cgctccttgc cgacggcgcg caccttgatc
     7321 agcatgagct cgcgctcggt gaactggccc tcggtgaggt cgacgacctt gacgacgtcg
     7381 atgaggcggt tcaggtgctt ggtgatctgc tcgatgacct cgtccgagcc gctcgtgacg
     7441 accgtcatgc gcgacagcga cggatcctcg gtcgtcgcga ccgtcaggct ctcgatgttg
     7501 tagccgcgcg ccgagaacag cgcgacgacc cgcgacaggg cgccgggctc gttctcgagc
     7561 agcaccgcga tgatgtgttt catgtccgtg tcccgccgcg tgtcctgccc ggccggcggt
     7621 aaccggtcgg gccttgcgcg gcgcctttcg aatcagggat ggaagaagaa tgtccgtcgg
     7681 acctcgacgc ccccgttgtg gtgccgctcc ggccccgggc ggcaacccgg agcctggcgg
     7741 acgcctcgcg ggcccgtcct cagaggtctt ccgaacccag cagcatctcc gtgatgccct
     7801 tgcccgcctt gaccatcggc cagacgttct cggtgggatc ggtctgcacg tcgatgaaca
     7861 ccgtgcgatc cttcaaggcg atcgcttcgc gcagcgccgg ctcgacgtct tccggcttcg
     7921 agatgcgcag gccgacgtgg ccgtaggcct cggcgagctt cacgaagtcg ggcagcgcat
     7981 ccatgtagct gtgcgagtag cggccgctgt attccagctg ctgccactgg cggaccatcc
     8041 ccaggtagcg gttgttcagg gagatgatct tcaccggcgt gttgtattgc tggcaggtgg
     8101 aaagctcctg gatgcacatc tggatggagc cctcgcccgt gatgcagaag acgtccgact
     8161 ggggcctggc cagcttgatg cccatcgcat agggcaggcc cacgcccatc gtgcccaggc
     8221 cgccggagtt gatccagcgt cgcggctcgt cgaagcggaa gtactgcgcg gcccacatct
     8281 ggtgctggcc gacgtcggag gtcacgtagc agtcgcggcc gcgcgtgaga tcccacaacg
     8341 cctcgaccac ctgctgcggc ttgatgacgt ccgggctgcg cttgtaggcg aggcagtcct
     8401 tgcggcgcca ttcgttgatc tggctccacc actgcgagac gaccgccgtg tccggcctcg
     8461 cctggccctc gcggatctgg gcgatcagct cctgcagcac gtccttgacg tcgccgacga
     8521 tggggatgtc gaccttgacg cgcttggaga tcgacgaggg gtcgatgtcc acgtggacga
     8581 tcttgcgctc gaccgacgcg aagtggcgcg ggttgccgat cacgcggtcg tcgaagcggg
     8641 cgccgatggc cagcaggacg tcgcagttct gcatggccat gttggcctcg aaggtgccgt
     8701 gcatgcccag catgcccagg aacttcggat cggtcgccgg gaaggcgccc aggcccatca
     8761 gcgtgttggt gaccgggtag ccgagcaggt cggcgagctc gcgcagctcg gccgccgcgt
     8821 tgccgagcac cacgccgccg cccgagtaga tgtagggccg gcgcgcgttc agcagcagct
     8881 gcacggcctt gcggatctgg ccgccgtggc ccttcttgac cgggttgtac gagcgcatct
     8941 cgacgtgctc ggggtagtgg tacggcgcca ccgcctgcga gacgtccttc gggatgtcga
     9001 ccacgaccgg gccggggcgg ccggtgcggg cgatgtggaa ggccttcttg atcgtcgagg
     9061 ccagctcgcg cacgtccttg accaggaagt tgtgcttgac gatcggccgc gtgatgccga
     9121 cggtgtcgca ctcctggaag gcgtcgagcc cgatcgccgg cgtcggcacc tgcccggtga
     9181 tgatcaccat cgggatggag tccatgtacg ccgtcgcgat gccggtgacc gcgttggtga
     9241 cgccggggcc cgaggtgacc agcgcgacgc cgacctcgcc ggtcgcgcgc gcgtagccgt
     9301 cggccgcgtg cacggcgccc tgttcatgac ggacgaggac gtgctggatg gcgtcctgct
     9361 tgtacagcgc gtcgtagatg tacaacacgg cgccgccggg gtagccccag acgaacttga
     9421 cgttctcggc ttgcaggcag cgcacgagga tctcggagcc gttcgggtcc gaggccgcgg
     9481 gagcggtcgt gggggaggaa atgggatgcg gacttccgga agcctggcgc ttcccgtccg
     9541 gggcagacat gtccatgggg aacctctgtg aatttctctg acgaaaaacc atcggtgctc
     9601 ctcttctcgc cctcgtgagg cggattcaga acctagagat cgaaaacagc cggcgcccgc
     9661 tttcgggtcg gcattcagac cagctcgcag ttgtgcgagt ggccattatt gcactgccgc
     9721 aacggaattg aacgagtttc agtgcatttg tcctccagcc ggcagcgttc ccggccgcga
     9781 gccgatagct gacggcgcgc tgaacgtccc cgccgcggcg aggcgccgcc ggcccgccgt
     9841 gcgcgggccg gcctcgtccg cgatgacata atccgccgcg tcatcagaca ggccgccctc
     9901 ctcgcgccgc gccctgcccc atcttggcca ccgacaaaga actctccgat ttcctcaaga
     9961 gcgtcgagaa gcgcgcgttc aagcgcacgg ccttcaccgt gcgcgacgac gacgccgccc
    10021 tcgacatcgt ccaggactcg atgatccggc tggcggagaa gtacgtcgac cggccggcgg
    10081 ccgagctgcc gctgctgttc cagcgcatcc tgtccaacgc cacgatggac tggttccggc
    10141 gccagaaggt gcgcaacgcc gtcgtgaaca atttctcgga cttcgagggc aacgacgacg
    10201 acggcgactt cgacatcctc gaaatgctgg acagcgtcga gggaaccctg ggcaccgaaa
    10261 gtgcatccga cgccgtgtcc cgagaccaga tcttgctgct gattgaaggc gagatcggcg
    10321 agctgccggc gcgtcaacgc gaagccttcc tcctgcgtta ctgggaggaa ctcgacgttg
    10381 ccgagacggc ctccgtgatg ggatgctcgg aaggcagcgt caagacgcac tgctcgcgcg
    10441 ccgtccacgc gctggccaag gcgttgaaag ccaagggaat cgcaccatga ccaggaacac
    10501 gcatcagacc ttcacgcacg ccccggacag ggacggcctg gaggcgcggt tcggcgtgcg
    10561 cgtcgcggcc gtgctgagcg agcgtgcgaa cgccaccgcc cccgacatct ccgagcgact
    10621 gcgcttcgcg cgcgagcagg cgctggccc
//
LOCUS       DASVLN010000918         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_533848, whole genome shotgun sequence.
ACCESSION   DASVLN010000918 DASVLN010000000
VERSION     DASVLN010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533848"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VF453_21530"
     CDS             <1..1055
                     /locus_tag="VF453_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690302.1"
                     /translation="AAGSGDIVLDNAANVLTGQVTASGHAVTLAAASALDADVQASGD
                     TSLTSAAGVAAQGSFGGALTATGSSVEIGEGSASQSTAIGGKLTAKATGGDVTLGNVQ
                     ANGASITASGAALLDGTLGSSGNVDITAGAIRGMNSRGALDVTAGHSTLLKATSGSIG
                     KTPGSLDGVVDKVGLITLLDVTGQNSSTLTVEINADQTAWFGVTSKSVIQGLIENTSL
                     NSHTFGCDANECFNVLGQTTAIADSVIANILTSAAQDAADAAFGTDNLDFAIRKGYVT
                     TIGRVPPGIDEIAGDLGATQCDTRVTSPTAIAADKACSSAAVTPRVDVKTQSQTAPEA
                     KPDPKKLSDTGHLIVY"
     gene            1141..>2170
                     /locus_tag="VF453_21535"
     CDS             1141..>2170
                     /locus_tag="VF453_21535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7690303.1"
                     /translation="MSDVPEKLGKYQIRRAIGKGAMGIVYEGFDPIIDRVVAIKTIKK
                     DELDKEEAEEHSRRFLIEAKAAGRLNHKNIVGIYDYGEERGLSFIVMEFVQGKELKSF
                     FNAKHPFSMSQIVRLMGELLDALGYSHSRGVIHRDVKPDNIFVTDDGSVKLGDFGIAR
                     IDSTQKTHAGTVLGTPSYMAPEQIRGETADARSDLYAAGVILYQFLVGERPFSGGMVQ
                     VMQKVLMEAPKPPSSHNPSIPEAIDAVVLRALAKQADQRFANAAEFSRELTAAVGLAS
                     EDEGESTMIMSADALKALQDAALAPADRTGTHGTQGTGSTRTGSTQTGTGTGTGGRTA
                     GSAGGAAGG"
BASE COUNT          378 a          781 c          722 g          289 t
ORIGIN      
        1 tggccgccgg ctcgggcgac atcgtcctcg acaacgccgc caacgtgctg accggccagg
       61 tcaccgcctc gggccacgcg gtcacgctgg ccgccgcctc ggcgctcgac gccgacgtgc
      121 aggcctccgg cgacacgtcg ctgacgtccg cggcgggcgt cgcggcgcag ggcagcttcg
      181 gcggcgcgct caccgccact ggcagctcgg tcgagatcgg cgagggcagc gcgagccagt
      241 cgacggcgat cggcggcaag ctcacggcca aggccacggg cggtgacgtc acgctcggca
      301 acgtgcaggc caacggcgcc agcatcaccg ccagcggcgc cgcgctgctc gacggcacgc
      361 tcggctcgtc gggcaacgtc gacatcacgg ccggcgcgat ccgcggcatg aacagccgcg
      421 gcgccctcga cgtcacggcc ggccactcga cgctgctgaa ggcgacttcc ggctcgatcg
      481 gcaagacgcc gggctcgctg gacggcgtcg tcgacaaggt cggcctgatc acgctgctcg
      541 acgtcacggg ccagaacagc tcgacgctga cggtggagat caatgcggac cagacggcct
      601 ggttcggcgt cacgtccaag tcggtgatcc agggcctgat cgagaacacg tcgctgaact
      661 cgcacacctt cggctgcgac gccaacgagt gcttcaacgt cctcggccag accacggcga
      721 tcgcggactc ggtcatcgcc aacatcctga cctcggcggc gcaggacgcg gcggacgcgg
      781 ccttcggcac ggacaacctg gacttcgcga tccgcaaggg ctacgtgacc accatcggcc
      841 gcgtgccgcc gggcatcgac gaaatcgcgg gcgacctcgg cgcaacgcaa tgtgacactc
      901 gggtaacatc gccgacggcc atcgcggcgg acaaggcgtg ctcctccgcg gcggtcactc
      961 cgcgggtgga cgtcaagacc cagtcccaga cggcgcccga ggccaagccc gaccccaaga
     1021 agctctccga caccggccac ctgatcgtct actgaccctg gcgggatccg tcgtcccccg
     1081 acggcgcccc ccgatgcccg gacttgatcc gggcatcgtc gtttacctgt tgcaagtcct
     1141 ttgagcgacg tacccgaaaa actgggcaag taccagatcc gccgagcgat cggcaagggt
     1201 gcgatgggca tcgtctatga gggattcgat ccgatcatcg accgggtcgt ggccatcaag
     1261 acgatcaaga aggacgagct cgacaaggag gaagccgagg agcactcccg gcgcttcctg
     1321 atcgaggcga aggccgccgg ccgcctcaac cacaagaaca tcgtcggcat ctacgactat
     1381 ggcgaggagc gcgggctctc gttcatcgtc atggagttcg tgcagggcaa ggagctgaag
     1441 tccttcttca acgccaagca cccgttctcg atgtcgcaga tcgtccgcct gatgggcgag
     1501 ctgctcgacg cgctgggcta ctcgcacagc cgcggcgtca tccaccgcga tgtcaagccc
     1561 gacaacatct tcgtcaccga cgacggctcg gtgaagctcg gcgacttcgg catcgcccgt
     1621 atcgactcca cgcagaagac gcacgccggc acggttctgg gcacgcccag ctacatggcg
     1681 cccgagcaga tccgcggcga gacggccgat gcccgttccg acctgtacgc cgcgggcgtg
     1741 atcctctacc agttcctggt cggcgagcgg ccgttctcag gcggcatggt gcaggtgatg
     1801 cagaaggtgc tgatggaggc gcccaagccg ccctcgtcgc acaacccgtc gatccccgag
     1861 gcgatcgatg ccgtcgtcct gcgcgcgctg gccaagcagg ccgaccagcg cttcgccaac
     1921 gcggccgagt tcagccgcga gctgaccgcc gcggtcggac tcgcgagcga ggacgagggc
     1981 gagtcgacga tgatcatgtc ggccgacgcg ctcaaggcgc tgcaggatgc cgccctcgcg
     2041 ccggccgacc gcacgggcac ccacggcacg cagggcaccg gcagcacgcg caccggctcc
     2101 acccagaccg gtacgggtac gggtaccggc ggacgcaccg cgggctcggc cggcggcgcc
     2161 gcgggcggcc
//
LOCUS       DASVLN010000919         9236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_46373, whole genome shotgun sequence.
ACCESSION   DASVLN010000919 DASVLN010000000
VERSION     DASVLN010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9236
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46373"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF453_21540"
     CDS             complement(<1..253)
                     /locus_tag="VF453_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7690304.1"
                     /translation="MAIIEVKVPQLSESVTEATLLQWKKKPGDAVAVDEILIEVETDK
                     VVLEVPAPSAGVLTEIVQADGATVTADQLIAKIDTEATAG"
     gene            complement(276..3134)
                     /locus_tag="VF453_21545"
     CDS             complement(276..3134)
                     /locus_tag="VF453_21545"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX7690305.1"
                     /translation="MMEQNRSNSYLFGGNAPYVEEMYEAYLENPGSVPDTWRAYFDAL
                     QHVPATDGSEARDVPHAPVIESFAQRAKANAFAVKTSSADLAIARKQVHVQSLIAAYR
                     FLGSRWADLDPLKRTERPKIAELEPAFYDLTEADMDITFSATNTYFTTSENMTLRQIV
                     QALRDTYCGTVGAEFMHITDPGEKRWWQERLEAARSKPVFTAEQKKHILDRLTAAEGL
                     ERYLHTKFVGQKRFSLEGGESFIVSMDELVQRAGAKGVQEVVIGMAHRGRLNVLVNTL
                     GKMPKDLFAEFDHTAPEDLPSGDVKYHQGFSSDISTPGGPIHLSLAFNPSHLEIVNPV
                     AEGSVKARMDRRGDVKGTQVLPVIVHGDAAFAGQGVVMETLALAQTRGYYTGGTVHLV
                     INNQIGFTTSDPRDTRSTLYCSDVVKMIEAPVLHVNGDDPEAVVLCTQLALDYRQQFN
                     KDVVVDIVCFRKLGHNEQDTPSLTQPLMYKKIGQHPGTRKLYGDKLVAQGILGETGPD
                     DMVKEFRAAMDAGRHTRDPVLTNFKGKYAVDWSPFLGKKWTDAADTALPVAEFKRLAE
                     RITTIPSNFKLHPLVEKVIADRAAMGRGEINVDWGMGEHLAFASLVASGYPVRLSGED
                     SGRGTFTHRHAVLHDQNREKFDTGTYIPLQNVAENQSPFVVIDSILSEEAVLAFEFGY
                     ASSDPNTLVIWEAQFGDFVNGAQVVIDQFIASGEVKWGRANGLTMMLPHGYEGQGPEH
                     SSARLERFMQLAADTNMQIVQPTTASQIFHLLRRQMVRAFRKPLVIMTPKSLLRNKDA
                     TSPMSEFTKGEFRTVIPELDASIDAAKVKRVIACSGKVYYDLVKKREEKKATDTAIIR
                     VEQLYPFPHKAFQAEIKKYSQATDIVWCQDEPQNQGAWFFVQHYIHENMLEGQKLGYA
                     GRPASASPAVGYAHLHQEQQKALLEQAFAKLKGFVLTK"
     gene            3395..3712
                     /locus_tag="VF453_21550"
     CDS             3395..3712
                     /locus_tag="VF453_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690306.1"
                     /translation="MSRQIIRLHPDAPRKPPEAAACNGCGVCCAAEPCPIGVLVSRRR
                     HGTCRALGWNEAAGRYRCGLAADPAAVLPWLPRGLAPVVRRLALRWISAASGCDSDLV
                     VEQ"
     gene            complement(3777..4763)
                     /locus_tag="VF453_21555"
     CDS             complement(3777..4763)
                     /locus_tag="VF453_21555"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX7690307.1"
                     /translation="MNAPLPTTARRELPAALRRLRLPVIGSPLFIISNPKLVIEQCKA
                     GIVGSMPALNARPAEQLDEWLAEITETLAAWDRDHPEAPSAPFAINQIVHKSNDRLEH
                     DMAMCAKYKVPIVITSLGARTDVNDAVHAWGGIVLHDIINDTFAHKAIEKGADGLIAV
                     AAGAGGHAGVKSPFALVQEIRQWFDGPVALSGAIATGDAVLAAQAMGADFAYIGSAFI
                     ATDEARAAEGYKQMIVDSTSDDIVYSNLFTGVLGNYLRGSITAAGLDPENLPTSDASK
                     MNFAAAGAAKAWKDIWGAGQGVGAIKEIVPAAKLVERLAREYAAARRRLALG"
     gene            complement(4766..6556)
                     /locus_tag="VF453_21560"
     CDS             complement(4766..6556)
                     /locus_tag="VF453_21560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7690308.1"
                     /translation="MTYRAPVKDMLFAMKELAGIDELTKLPAFADAGIDTAQAVLEEC
                     AKFNEDVVAPLNVPGDREPTSLKDGVVTATRGFKEAWRQFAEGGWQGLQHPADFGGQG
                     LPKLIGAACGEMINSANVSFALCPLLTDGAIEALLTAGSDEQKATYLPRMLSGEWTGS
                     MNLTEPQAGSDLSLVRTRAEPQPDGTYKVFGTKIFITWGEHDMADNIVHLVLARVAGA
                     PEGVKGISLFIVPKFLVGADGALGPRNDVHCVSIEHKLGIKASPTCVLQFGDHGGATG
                     YLVGQENRGLEYMFIMMNAARFGVGVQGIAVAERAYQHAVAYARERVQSRPVDGSMTA
                     AASIIHHPDVRRMLMTMRAYTEACRAMALVAASAYDAAHAHPDASVRADNQAFYEFMV
                     PLVKGYSTEMSLEVASLGIQVHGGMGFIEETGAAQYLRDSKILAIYEGTTAIQANDFV
                     GRKTARDGGTTAKAIAARIAATEAELAASANADAQAFGKRLKAAREAFLDAVDFIAAN
                     AKASPNEAFAGSVPYLMLAGNLVAGWQMGRALLAAAAHVAAGEDAAFHAAKIATARFY
                     GDHILSKAPGVRDSIVDGAAGTLALPLEAF"
     gene            complement(6631..7563)
                     /locus_tag="VF453_21565"
     CDS             complement(6631..7563)
                     /locus_tag="VF453_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX7690309.1"
                     /translation="MTTLVIAEHDNATLKAATLNTITAAAQVGGDVHVLVAGANAGAA
                     AQAAAQVAGVAKVLHADAPGFAEFLAENVAAQVLAVAPGYSHIFFPATANGKNVAPRV
                     AARLDVAQLSDITKVVSPDTFERPIYAGNAIATVQCGQAVKVATVRGTAFDAAAATGG
                     SAAVESVAPVADSGRSAFVGKEIAKSDRPELTAAKIIVSGGRAMGSSEKFNEVLTPLA
                     DKLGAALGASRAAVDAGYAPNDWQVGQTGKIVAPQLYVACGISGAIQHLAGMKDSKVI
                     VAINKDEEAPIFSVADYGLVADLFVAVPEMINAL"
     gene            complement(7596..8345)
                     /locus_tag="VF453_21570"
     CDS             complement(7596..8345)
                     /locus_tag="VF453_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX7690310.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKSDGTGVDIANVKMSMNPFDEIAVE
                     EAVRLKEKGVVTEVVAVSCGVAKCEETLRTAMAIGADRAILVETEVELEPLAVAKLLK
                     ALVDKEQPQLVILGKQAIDDDCNQTGQMLAALADLPQATFASKVEVADGKATVTREVD
                     GGLETLALTLPAIVTTDLRLNEPRYVTLPNIMKAKKKPLDKTDPATLGVDVAPRLKTL
                     KVAEPPKRGAGVKVPDVATLVGKLKNEAKVI"
     gene            8613..>9236
                     /gene="murI"
                     /locus_tag="VF453_21575"
     CDS             8613..>9236
                     /gene="murI"
                     /locus_tag="VF453_21575"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX7690311.1"
                     /translation="MFDSGVGGLSVLRAIRRRLPQAELLYAADTGFAPYGDRPEADIH
                     ERSRHVVGFLREHGAQVVVIACNTATTVAVRALRDIDGMLPIVGVEPGIKPAVTLSAA
                     RRVGVLATTRTVSSDRVRSLVRDHSAGAQVLLQACPGLVDAIESPATGEATLASLVER
                     YSAPLRDAGVDVAVLGCTHYAFARRLFERALPGVHFVDTADAVARQTA"
BASE COUNT         1237 a         3140 c         3264 g         1595 t
ORIGIN      
        1 tgcccgcggt cgcctccgtg tcgatcttgg cgatcagctg gtcggcggtg acggtggcgc
       61 cgtcggcctg gacgatctcg gtcagcacgc cggcggacgg cgccggcacc tcgagcacga
      121 ccttgtcggt ctcgacttcg atcaggatct cgtccacggc gacggcgtcg ccgggcttct
      181 tcttccactg cagcagggtc gcttcggtca cggattccga cagctgcgga accttcactt
      241 cgatgatggc catgagttgt tctctgtctt tcgaattact tggtcaggac gaagcccttg
      301 agcttggcga acgcctgctc gagcagcgcc ttctgctgct cctggtgcag gtgggcgtag
      361 ccgacggccg gcgaggcgga ggccgggcgg ccggcgtagc cgagcttctg gccctcgagc
      421 atgttctcgt ggatgtagtg ctgcacgaag aaccaggcgc cctggttctg cggctcgtcc
      481 tggcaccaca cgatgtccgt ggcctgcgag tacttcttga tctcggcctg gaaggccttg
      541 tgcgggaacg gatagagctg ctcgacgcgg atgatggccg tgtcggtcgc cttcttctcc
      601 tcgcgcttct tgacgaggtc gtagtagacc ttgcccgagc acgcgatcac gcgcttgacc
      661 ttggccgcgt cgatcgaggc gtcgagctcg gggatcaccg tgcggaactc gcccttggtg
      721 aactcgctca tcggcgacgt ggcgtccttg ttccgcagca gcgacttcgg ggtcatgatg
      781 accagcggct tgcggaacgc gcgcaccatc tggcggcgca gcaggtggaa gatctggctg
      841 gccgtggtcg gctgcacgat ctgcatgttg gtgtcggcgg ccagctgcat gaagcgctcc
      901 aggcgcgcgg agctgtgctc cgggccctgc ccctcgtagc cgtgcggcag catcatcgtc
      961 aggccgttgg cgcggcccca cttgacctcg cccgaggcga tgaactggtc gatcacgacc
     1021 tgcgcgccgt tgacgaagtc gccgaactgc gcctcccaga tcaccagcgt gttcggatcc
     1081 gacgaggcgt agccgaactc gaacgcgagc acggcctcct ccgacaggat cgagtcgatc
     1141 acgacgaagg gcgactggtt ctcggcgacg ttctgcagcg ggatgtaggt gccggtgtcg
     1201 aacttctcgc ggttctggtc gtgcagcacc gcgtggcggt gcgtgaacgt gccgcggccg
     1261 gaatcctcgc ccgacaggcg taccgggtag ccgctcgcga ccagcgaggc gaacgccagg
     1321 tgctcgccca tgccccagtc gacgttgatc tcgccgcggc ccatggcggc gcggtcggcg
     1381 atgaccttct ccaccagcgg atgcagcttg aagttcgacg ggatggtcgt gatgcgctcg
     1441 gccagccgct tgaactccgc gaccggcagt gcggtgtcgg cggcgtccgt ccacttcttg
     1501 cccaggaacg gcgaccagtc gaccgcgtac ttgcccttga agttggtcag gacggggtcg
     1561 cgggtgtggc ggccggcgtc catcgcggcg cggaactcct tgaccatgtc gtccggcccg
     1621 gtctcgccga ggatgccctg ggccaccagc ttgtcgccgt acagcttgcg cgtgcccggg
     1681 tgctggccga tcttcttgta catcagcggc tgcgtcaggc tgggcgtgtc ctgctcgttg
     1741 tggcccagct tgcggaagca gacgatgtcg acgaccacgt ccttgttgaa ctgctggcgg
     1801 tagtcgagcg cgagctgcgt gcacagcacg accgcttcgg gatcgtcgcc gttcacgtgc
     1861 agcaccgggg cctcgatcat cttgacgacg tccgagcagt acagcgtgga gcgggtgtcg
     1921 cgcgggtcgg aggtggtgaa gccgatctgg ttgttgatga ccaggtgcac cgtgccgccg
     1981 gtgtagtagc cgcgcgtctg cgcgagcgcc agcgtctcca tcacgacgcc ctggcccgcg
     2041 aaggcggcgt cgccgtggac gatcaccggc agcacctggg tgcccttcac gtcgccgcgg
     2101 cggtccatgc gcgccttgac ggagccctcg gcgaccgggt tgacgatctc caggtgcgac
     2161 gggttgaacg cgagcgacag gtggatcggg ccacccgggg tcgagatgtc gctggagaag
     2221 ccctggtggt acttcacgtc gcccgagggc aggtcctccg gcgcggtgtg gtcgaactcg
     2281 gcgaacaggt ccttcggcat cttgccgagg gtgttgacca gcacgttcag gcggccgcgg
     2341 tgggccatgc cgatgacgac ttcctgcacg cccttggcgc cggcgcgctg caccagctcg
     2401 tccatcgaga cgatgaagct ctcgccgcct tccagcgaga agcgcttctg gccgacgaac
     2461 ttggtgtgca ggtagcgctc gaggccttca gccgcggtca ggcggtcgag gatgtgcttc
     2521 ttctgctccg ccgtgaagac cggcttgctg cgcgccgcct ccaggcgctc ctgccaccag
     2581 cgcttctcgc cggggtcggt gatgtgcatg aactcggcgc cgacggtgcc gcagtacgtg
     2641 tcgcgcagcg cctggacgat ctggcgcagc gtcatgttct cgctggtcgt gaaatacgtg
     2701 ttcgtcgccg agaacgtgat gtccatgtcg gcttcggtga ggtcgtagaa cgcgggctcc
     2761 agctcggcga tcttcgggcg ctcggtgcgc ttgagcgggt cgaggtcggc ccagcgcgag
     2821 ccgaggaagc ggtaggccgc gatcagcgac tggacgtgga cctgcttgcg cgcgatcgcg
     2881 aggtcggccg agctcgtctt gaccgcgaag gcattggcct tggcgcgctg cgcgaaggac
     2941 tcgatgaccg gcgcgtgggg aacgtcgcgg gcctcggagc cgtcggtcgc cgggacgtgc
     3001 tgcagcgcgt cgaagtaggc gcgccaggtg tccggcaccg agccggggtt ctcgagatag
     3061 gcctcgtaca tctcctccac gtacggcgcg ttgccgccga acaggtagga gttggacctg
     3121 ttttgctcca tcatgtttcg ctcacctttc gccccggttt ccggagctcc gatgggttga
     3181 tcaaccttcc gcgggcccgg ctcgaccggt tggcggattg caagcgcctg cttgccggac
     3241 tggagaggcc ggcaaggcgc agaaggactc ttggactgcg ttccgggcgt gaatgtagca
     3301 cttacccgga gtccatgccc gcaacgaact gcgcccggac ggccgcgttt cttgcaggaa
     3361 cgcacgatcg cgggcggcgc gacactggcc aggcatgtcc cgccagatca tccgcctgca
     3421 ccccgacgcc ccgcgaaagc cgcccgaggc cgccgcctgc aacggctgcg gcgtgtgctg
     3481 cgcggccgag ccgtgcccga tcggcgtcct ggtgtcgcgc cggcgccacg gtacctgccg
     3541 cgcgctcggc tggaacgagg ccgccggccg ctaccgctgc gggctggccg ccgatcccgc
     3601 cgccgtcctg ccctggctgc cgcgcgggct cgctcccgtc gtgcgccgcc tcgccctgcg
     3661 ctggatctcg gcggcgagcg gttgcgacag cgacctcgtc gtcgagcagt gaaccgcgga
     3721 caaaaaaagg gcgccccgaa ggacgcctgc aagacctctt gaaccgggac gggccgtcag
     3781 cccagggcga gccgccggcg cgcggccgcg tactcgcgcg cgaggcgctc gacgagcttg
     3841 gcggccggca cgatttcctt gatcgcgccc acgccctggc ccgcgcccca gatgtccttc
     3901 caggccttgg cggcgccggc ggcggcgaag ttcatcttgc tcgcgtcgct ggtgggcagg
     3961 ttctcgggat ccagcccggc cgcggtgatc gagccgcgca ggtagttgcc gagcacgccc
     4021 gtgaacaggt tggagtagac gatgtcgtcg ctcgtcgagt ccacgatcat ctgcttgtag
     4081 ccttcggcag cgcgtgcctc gtcggtggcg atgaaggccg agccgatgta ggcgaagtcc
     4141 gcgcccatcg cctgcgccgc gagcaccgcg tcgcccgtgg cgatcgcgcc cgagagcgcg
     4201 accgggccgt cgaaccactg gcggatctcc tgcaccagcg cgaacgggct cttcacgccc
     4261 gcgtggccgc cggcgccggc ggccaccgcg atcaggccgt ccgcgccctt ctcgatcgcc
     4321 ttgtgcgcga aggtgtcgtt gatgatgtcg tgcagcacga tgccacccca ggcgtgcacg
     4381 gcgtcgttga cgtcggtgcg cgcgcccagc gaggtgatga cgatgggcac cttgtacttg
     4441 gcgcacatgg ccatgtcgtg ctcgaggcgg tcgttgctct tgtgcacgat ctggttgatc
     4501 gcgaacggcg cggacggcgc ctccggatgg tcgcgatccc aggcggcgag ggtttcggtg
     4561 atctcggcga gccactcgtc gagctgctcg gccggccgcg cgttgagtgc cggcatcgag
     4621 ccgacgatgc cggccttgca ctgctcgatc acgagcttgg ggttgctgat gatgaacagc
     4681 ggcgagccga tcaccggcag gcgcagcctg cgcagcgcgg cgggcagctc tcggcgggcg
     4741 gtcgtgggca ggggtgcgtt catggtcaga aggcctccag gggcagggcg agcgtgccgg
     4801 cggcgccgtc gacgatgctg tcgcgcacgc ccggggcctt gctcaggatg tggtcgccgt
     4861 agaagcgcgc ggtcgcgatc ttggcggcat ggaaggccgc gtcctcgccc gcggcgacat
     4921 gcgcggccgc ggccagcagg gcgcggccca tctgccagcc ggcgacgagg ttgcccgcca
     4981 gcatcaggta cggcaccgag ccggcgaagg cctcgttcgg gctcgccttc gcgttggcgg
     5041 cgatgaagtc gacggcgtcg aggaaggcct cgcgcgccgc cttcaggcgc ttgccgaagg
     5101 cctgcgcgtc cgcattcgcc gaggccgcga gctcggcttc ggtcgcggcg atgcgcgcgg
     5161 cgatcgcctt cgcggtcgtg ccgccgtcgc gcgccgtctt gcggccgacg aagtcgttgg
     5221 cctggatcgc ggtggtgccc tcgtagatcg cgaggatctt cgagtcgcgc aggtactgcg
     5281 ccgcgccggt ctcctcgatg aagcccatgc cgccgtgcac ctggatgccc aggctcgcga
     5341 cctccaggct catctcggtg gagtagccct tgaccagcgg caccatgaac tcgtagaagg
     5401 cctggttgtc ggcgcgcacg gacgcgtcgg gatgcgcgtg cgcggcgtcg taggccgagg
     5461 cggcgaccag cgccatcgcg cggcaggcct cggtgtaggc gcgcatcgtc atcagcatgc
     5521 ggcgcacgtc ggggtgatgg atgatcgatg ccgccgccgt catcgagccg tcgaccggac
     5581 gcgactgcac gcgctcgcgc gcgtaggcga ccgcgtgctg gtaggcgcgc tcggccaccg
     5641 cgatgccctg cacgccgacc ccgaagcggg cggcgttcat catgatgaac atgtactcga
     5701 ggccgcggtt ctcctggccg accaggtagc cggtcgcccc gccgtggtcg ccgaactgca
     5761 gcacgcaggt cgggctcgcc ttgatgccca gcttgtgctc gatgctcacg cagtgcacgt
     5821 cgttgcgcgg gcccagcgcg ccgtccgcgc ccacgaggaa cttcggcacg atgaacagcg
     5881 agatgccctt gacgccctcg ggcgcccccg cgacgcgcgc gagcacgagg tggacgatgt
     5941 tgtccgccat gtcgtgctcg ccccaggtga tgaagatctt cgtgccgaag accttgtagg
     6001 tgccgtcggg ctgcggctcg gcgcgcgtgc gcacgagcga caggtcggag ccggcctgcg
     6061 gctcggtgag gttcatcgag cccgtccact cgcccgacag catccggggc aggtacgtcg
     6121 ccttctgctc gtcggagccc gcggtcagca gcgcctcgat cgcgccgtcg gtcagcaagg
     6181 ggcacagcgc gaagctcacg ttggccgagt tgatcatctc gccgcaggcc gcgccgatca
     6241 gcttgggcag gccctggccg ccgaagtcgg ccggatgctg caggccctgc cagccgccct
     6301 cggcgaactg cctccaggct tccttgaagc ccctggtcgc ggtgacgacg ccgtccttca
     6361 gcgaggtggg ctcgcggtcg cccggcacgt tcagcggggc gaccacgtcc tcgttgaact
     6421 tcgcgcattc ctcgagcacc gcctgggcgg tgtcgatgcc ggcgtcggcg aacgcgggca
     6481 gcttggtgag ttcgtcgatg ccggccagct ccttcatcgc gaagagcatg tccttgacgg
     6541 gcgctcggta ggtcatctgt cagtctccgg gggcaaaaaa aggggcgcct gcgcgaggtc
     6601 gccccgtgtg gtcgtcgcgt cgcggcgcgc tcagagcgcg ttgatcatct cgggcacggc
     6661 gacgaacagg tccgccacca ggccgtagtc ggccaccgag aagatcggcg cctcctcgtc
     6721 cttgttgatc gccacgatga ccttggagtc cttcatgccg gccaggtgct ggatcgcgcc
     6781 cgagatgccg cacgcgacat acagctgcgg cgcgacgatc ttgccggtct ggccgacctg
     6841 ccagtcgttg ggcgcgtagc ccgcgtcgac cgccgcgcgc gaggcgccga gcgcggcgcc
     6901 cagcttgtcg gccagggggg tcagcacttc gttgaacttc tcgctcgagc ccatcgcgcg
     6961 gccgcccgag acgatgatct tggcggccgt cagctccggc cggtcgctct tggcgatctc
     7021 cttgccgacg aaggcgctcc tgcccgagtc ggccaccggc gcgacgctct ccaccgcggc
     7081 cgagccgccg gtcgcggcgg cggcgtcgaa ggcggtgccg cgcaccgtcg cgaccttgac
     7141 cgcctggccg cactgcaccg tggcgatcgc gttgccggcg tagatcgggc gctcgaaggt
     7201 atcgggcgag acgaccttgg tgatgtcgct cagctgcgcg acgtcgagcc tggcggccac
     7261 gcgcggcgcg acgttcttgc cgttggcggt cgcgggaaag aagatgtgcg agtagcccgg
     7321 cgcgacggcc agcacctgcg cggcgacatt ctcggccagg aactccgcga agcccggtgc
     7381 gtctgcgtgc agcaccttgg cgacgcccgc gacctgcgcg gcggcctggg cggccgcgcc
     7441 ggcgttcgcg ccggcgacca gcacgtgcac gtcgccgccg acctgggccg cggcggtgat
     7501 ggtgttgagc gtcgcggcct tgagcgtggc gttgtcgtgt tcggcgatga cgagggtggt
     7561 catgtgggac ggttctccgt gttcggggtg agcggtcaga tgaccttcgc ttcgttcttg
     7621 agcttgccga ccagcgtggc cacgtcgggc accttcacgc cggcgccgcg cttgggcggc
     7681 tcggcgacct tcagcgtctt caggcgcggc gcgacgtcga cgcccagcgt ggccgggtcg
     7741 gtcttgtcca gcggcttctt cttggccttc atgatgttgg gcagcgtgac gtagcgcggc
     7801 tcgttcaggc gcaggtcggt cgtcacgatg gcgggcagcg tcagcgccag cgtctcgagg
     7861 ccgccgtcga cctcgcgcgt cacggtggcc ttgccgtcgg cgacctcgac cttcgaggcg
     7921 aaggtggcct gcggcaggtc ggccagcgcg gcgagcatct ggcccgtctg gttgcagtcg
     7981 tcgtcgatcg cctgcttgcc gaggatcacg agctgcggct gctccttgtc gacgagcgcc
     8041 ttgagcagct tggccacggc cagcggctcg agctcgacct cggtctccac caggatcgcg
     8101 cggtcggcgc cgatggccat cgcggtgcgc agcgtctcct cgcacttggc gacgccgcac
     8161 gagacggcga cgacctcggt gacgacgccc ttctccttga gccgcaccgc ttcttcgacg
     8221 gcgatctcgt cgaacgggtt catgctcatc ttgacgttgg cgatgtccac gccggtgccg
     8281 tccgacttca cgcgaacctt cacgttgtag tcgacgacgc gtttcacagg caccagaacc
     8341 ttcatgacgc aaaactccta ggaagcaatt gacgttaacg taaactggaa tggattctag
     8401 agggacacgt cgctctgatg cccgtctcgt tgtccgatcg gtcgagaata aaacgaacga
     8461 tcgtgctatt ttagatgctt cgctccagct ttccaaccgc ttcgcgcgcc atgtcgctcc
     8521 gaccccaccc gatttcgccc gaccttggtg tttccccggg ggcggcaagg ggatcggacg
     8581 ccccgaccgc gcgtgcgccg ttgcgcatcg gcgtgttcga ctccggcgtg ggcgggctgt
     8641 cggtgctgcg ggcgatccgc cgccggctgc cgcaggccga gctgctctac gcggccgaca
     8701 cgggcttcgc accctacggc gaccgcccgg aggccgacat ccacgagcgc tcgcgccacg
     8761 tcgtgggctt cctgcgcgag catggcgcgc aggtagtggt gatcgcgtgc aacaccgcga
     8821 ccaccgtggc ggtgcgcgcg ttgcgcgaca tcgacgggat gctgcccatc gtcggcgtcg
     8881 agccgggcat caagccggcg gtcaccctga gcgcggcgcg gcgcgtgggc gtgctggcca
     8941 ccacgcgcac ggtaagcagc gaccgcgtgc gcagtctggt gcgcgaccac agcgccggcg
     9001 cgcaggtcct gctccaggcc tgcccgggcc tcgtcgacgc catcgagtcc ccggccaccg
     9061 gcgaggccac gcttgcctcg ctggtcgagc gctacagcgc cccgctgcgc gacgcgggcg
     9121 tcgacgtcgc ggtgctcggc tgcacccact acgcgttcgc gcgcaggctg ttcgagcgcg
     9181 ccctgcccgg cgtgcacttt gtcgacacgg ccgacgccgt cgcccgccag accgcc
//
LOCUS       DASVLN010000920         4861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_209301, whole genome shotgun sequence.
ACCESSION   DASVLN010000920 DASVLN010000000
VERSION     DASVLN010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209301"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2387
                     /locus_tag="VF453_21580"
     CDS             <1..2387
                     /locus_tag="VF453_21580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7690312.1"
                     /translation="DVATAASGEDGLRLAAQLRPSAIVVDGQLPGIDGMTVVRRVRLD
                     AALRGVPCLLLTGSHELSVELQALDAGADAFVHKDEEIPVILARLGAALRATERRAGD
                     GPSSLLAPKKVLAVDDSASYLHDLADVLREEGYDVVCARSGEQALELLSVQAVDCILM
                     DLQMPGLDGRETCLRIKGAPGVRDIPLIMLTALDDRAAMLAGLGAGADDYLQKSSEFE
                     VIKARVRAQLRRKQFEDENRRIREELLHEASETRAARELAQARAELVEQLERKTRELE
                     LATQAKSRFLAMMSHEIRTPLNAVIGMAGLLDKSELSDQQREYAGIIRTSGDHLLAVI
                     NDILDFSSLEFGGLALESRSLAVANVVEESLDLVAGAARAKEIELTYELAPGVPPTVL
                     GDAGRIRQILVNYLSNAVKFTARGEVVVTVDARSLPDGRHELHGAVRDTGRGIPRDRF
                     DRLFHSFSQVDASVRREFGGTGLGLAICKRLAELMGGRVWVESEAGRGSTFHFTVVAA
                     VPDEPSPAEPAAAPQPLTGVRAWIVDDNDANRLILRRQLQDWGLDIRDTGSPEEALAW
                     ARRGDACELAILDFHMPAMDGVQLARALHELRGPALKQVLLTSGLPVADGEARAAGLL
                     AQLSKPVKHRPLLNTVLKLFEGRVVANAARAPAVAPVTPPVPAASGGLRILVAEDNPI
                     NVYLLKILLEHAGFESDVVGNGQEAVDAVRERRYDVVFMDVQMPVMDGTEATRRILEQ
                     HPAGDRPRIIALTAGVMADEVQACRDAGVDDFLAKPIDPARLAASLARCRRRDD"
     gene            complement(2460..2939)
                     /locus_tag="VF453_21585"
     CDS             complement(2460..2939)
                     /locus_tag="VF453_21585"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7690313.1"
                     /translation="MTPALNKPLPEFEALATNGVKFTPHAFAGRAVVLYFYPKDHTPG
                     CTTEAMQFRDQHEEFTQAGAVVFGVSRDNMASHEKFKQGLSLPFELIADTEEKLCHMF
                     GVVKNKIMYGKKVKGIERSTFLIDGNGVLVAEWRGIKVADHVAEVLKAVKALQAKAA"
     gene            complement(3186..3569)
                     /locus_tag="VF453_21590"
     CDS             complement(3186..3569)
                     /locus_tag="VF453_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEX7690314.1"
                     /translation="MKFQPDSLAGVNAITHYDVDSLRVNGELHTASVLVPWVGTPVAW
                     NAASLDALGPAHFEALLALEPEVVIFGTGPRMKFVSPALYRSLIEHRVGIETMDSGAA
                     CRTYTVLANEGRRVVAAILLVPAAT"
     gene            3714..4832
                     /gene="mnmH"
                     /locus_tag="VF453_21595"
     CDS             3714..4832
                     /gene="mnmH"
                     /locus_tag="VF453_21595"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEX7690315.1"
                     /translation="MPAPTPRAPFVAPPADAAPPLAAVPVVGLERGREFDAVIDARSP
                     AEYALDHMPGALNCWVLDNEERHVVGTIYVQQGAFEARRVGGAMVAANLARHLQERFS
                     GMPMGWRPLVYCWRGGMRSGAMVSWLRTVGWDAAQLAGGYKTWRAHVMDAVEGVPPRL
                     RLTVIAGATGSAKTRVLQALAAHGAQVLDLEGLACHKGSLLGELPGVPQPSQKAFETG
                     LVDALERLDPARPVFVEGESRRIGRLQVPLALVQRMRAAPVVEIVAPFQARVDYLLRD
                     YAYLGDRPDWFAGQLRALTELHGKETIAAWQAMAQAHELAPLFAELAGRHYDPAYARS
                     QGVHFARWEARTRVEAPGLEDADIDALAARIAALPATD"
BASE COUNT          666 a         1738 c         1789 g          668 t
ORIGIN      
        1 acgacgtcgc caccgcggcc agcggcgagg acgggctgcg cctggccgcg cagctgcgcc
       61 cgagcgcgat cgtcgtcgac ggccagctgc ccggcatcga cggcatgacg gtcgtgcgcc
      121 gggtgcggct ggacgcggcg ctgcgcggcg tgccctgcct gctgctgacc ggctcgcacg
      181 aactgagcgt cgagctgcag gcgctcgacg ccggcgccga cgccttcgtc cacaaggacg
      241 aggagatccc cgtgatcctc gcccgcctgg gcgccgccct gcgcgccacc gaacgccgcg
      301 cgggcgacgg cccgtcgagc ctgctcgcgc cgaagaaggt gctggccgtc gacgacagcg
      361 cgagctacct gcacgatctc gccgacgtgc tgcgcgagga aggctacgac gtggtctgcg
      421 cgcgctccgg cgagcaggcg ctggagctgc tgtcggtgca ggcggtcgac tgcatcctga
      481 tggacctgca gatgcccggc ctcgacggcc gcgagacctg cctgcgcatc aagggcgcgc
      541 ccggcgtgcg cgacatcccg ctgatcatgc tgaccgccct cgacgaccgc gccgcgatgc
      601 tcgccggcct cggcgcgggt gcggacgact acctgcagaa gtcgtccgag ttcgaggtca
      661 tcaaggcgcg cgtgcgggcg cagctgcggc gcaagcagtt cgaggacgag aaccgccgca
      721 tccgcgagga gctgctgcac gaggcctccg agacgcgggc cgcgcgcgag ctcgcgcagg
      781 cgcgcgcgga gctggtcgag cagctcgagc gcaagacgcg cgagctggag ctcgcgacgc
      841 aggccaagag ccgcttcctc gcgatgatga gccacgagat ccgcacgccg ctgaacgccg
      901 tcatcggcat ggcgggactg ctggacaagt ccgagctgag cgaccagcag cgcgagtacg
      961 ccggcatcat ccgcacgagc ggcgaccacc tgctggccgt catcaacgac atcctcgact
     1021 tctcgagcct cgagttcggc ggcctcgcgc tggagagccg gtcgctggcg gtggccaacg
     1081 tcgtcgagga gtcgctggac ctcgtcgccg gcgccgcccg cgccaaggag atcgagctca
     1141 cctacgagct cgcgcccggc gtgccgccga cggtgctggg cgacgcgggc cgcatccggc
     1201 agatcctggt caactacctg tccaacgcgg tgaagttcac ggcgcggggc gaggtcgtcg
     1261 tcaccgtcga tgcgcggtcg ctgcccgacg gccgccacga gctgcacggc gcggtgcgcg
     1321 atacggggcg gggtataccc cgggatcgct tcgaccgcct gttccattcg ttcagccagg
     1381 tcgacgcgtc ggtgcggcgc gagttcggcg gcacgggact gggcctggcg atctgcaagc
     1441 ggctggccga gctcatgggc gggcgggtct gggtcgagag cgaggcgggc cgcggctcga
     1501 ccttccactt cacggtggtg gccgccgtgc cggacgagcc ctcgcccgcc gagccggcgg
     1561 ccgcacccca gccgctgacc ggcgtgcgtg cctggatcgt cgacgacaac gacgccaacc
     1621 gcctgatcct gcgccgccag ctgcaggact gggggctgga catccgcgac accggatcgc
     1681 ccgaggaggc gctggcctgg gcgcgccgcg gcgacgcgtg cgagctcgcc atcctcgatt
     1741 tccacatgcc cgcgatggac ggcgtgcagc tcgcgcgcgc gctccacgag ctgcgcggcc
     1801 cggcgctcaa gcaggtgctg ctgacctcgg gcctgcccgt cgcggacggc gaagcgcgcg
     1861 ccgcggggct gctggcgcag ctgtccaagc cggtcaagca ccgcccgctg ctcaacaccg
     1921 tgctcaagct gttcgagggg cgcgtggtcg ccaatgcggc acgcgccccg gccgtcgcgc
     1981 ccgtcacgcc gccggtgcct gcggcgagcg gcggcctgcg catcctcgtc gcggaagaca
     2041 acccgatcaa cgtctacctg ctcaagatcc tgctggagca cgcgggcttc gagtcggacg
     2101 tggtgggcaa cgggcaggag gcggtcgacg cggtgcgcga gcgccgctac gacgtcgtct
     2161 tcatggacgt gcagatgccc gtgatggacg gcaccgaggc gacgcgccgc atcctggagc
     2221 agcacccggc cggcgaccgc ccgcgcatca tcgcgctcac ggccggcgtg atggccgacg
     2281 aggtgcaggc ctgccgcgat gccggcgtcg acgacttcct cgccaagccg atcgaccccg
     2341 ccaggctcgc ggcctcgctg gcgcgctgcc ggcgccgcga cgactgacgg ccacggcggg
     2401 ggcgcgcagg cgaagaaaaa gccgcccggg ggcggcttcg tcgctggggc gcgcggcgct
     2461 caggcggcct tggcctgcag cgccttgacg gccttgagca cctcggccac gtggtcggcg
     2521 accttgatcc cgcgccactc ggccacgagc acgccgttgc cgtcgatgag gaaggtgctg
     2581 cgctcgatgc ccttgacctt cttgccgtac atgatcttgt tcttgaccac gccgaacatg
     2641 tggcagagct tctcctcggt gtcggcgatc agctcgaagg gcagggacag gccctgcttg
     2701 aacttctcgt gcgaggccat gttgtcgcgc gaaacgccaa aaacgaccgc acccgcctgc
     2761 gtgaactcct cgtgctggtc gcggaactgc atggcctcgg tggtacaacc cggcgtgtga
     2821 tccttcgggt agaagtagag caccacggcg cgcccggcga aggcatgcgg cgtgaacttg
     2881 acgccgttgg ttgcgagtgc ttcgaattcg gggagcggct tgttcagtgc aggcgtcatg
     2941 tagtgtctcg gtcgagccgt cggtcgatct gggggcgtac gagagatggg ctttccggac
     3001 ccgggcgacc gcagtcgtat ccggctctgg cacaacaacc gtcgattata gattgaactg
     3061 tccaccttcc tttgggggaa gcgggtgggt gcgcatgaat taccgaggcg ccgggcgcat
     3121 gcgcgggcgc acgcaattcg accggaaagc cgcgccggcc gggtgagccc ggcgagcgag
     3181 agggcttacg tcgctgcagg cacgagcagg atcgccgcga ccacgcgccg cccttcgttg
     3241 gcgagcaccg tgtaggtgcg gcaggcggcg ccgctgtcca tcgtctcgat gccgacgcgg
     3301 tgctcgatca ggctgcgata gagcgccggc gagacgaact tcatgcgcgg gccggtgccg
     3361 aagatcacga cctccggctc cagcgccagc agcgcctcga agtgggccgg gcccagcgcg
     3421 tccaggctcg cggcgttcca ggcgacgggc gtcccgaccc agggcacgag cacgctggcc
     3481 gtgtggagct cgccgttcac gcgcaggctg tcgacgtcgt aatgggtgat ggcgttcacc
     3541 ccggcgagcg agtcgggctg gaatttcacg gtcgtcggtc ctgggcaggc gagggcgggg
     3601 tgggcggccg attataggaa cgcgggctcc gccgccacgg ccgggcgcca agcggcccgc
     3661 cggccggtgg gagaatcccg gagtcccgcc gtccttccgc tcccgcccgc ccgatgcccg
     3721 ctcccacgcc ccgcgccccg ttcgtcgccc cgcccgcgga cgccgcgccg cccctggccg
     3781 cggtgcccgt cgtcgggctc gagcgcgggc gcgagttcga cgccgtgatc gacgcgcgct
     3841 cgcccgccga gtacgcgctc gaccacatgc cgggtgcgct caactgctgg gtgctggaca
     3901 acgaggagcg ccacgtcgtc ggcacgatct acgtgcagca gggcgcgttc gaggcacggc
     3961 gcgtcggcgg cgcgatggtc gcggccaacc tggcgcgcca cctgcaggag cgcttctccg
     4021 gcatgccgat gggctggcgg ccgctggtct actgctggcg cggcggcatg cgcagcggcg
     4081 cgatggtgag ctggctgcgc accgtcggct gggacgcggc gcagctcgcc ggcggctaca
     4141 agacctggcg cgcgcacgtg atggacgcgg tcgagggcgt cccgccgcgg ctgcggctga
     4201 ccgtgatcgc cggcgccacc ggcagcgcga agacgcgcgt gctgcaggcc ctggccgcgc
     4261 acggcgccca ggtgctggac ctcgagggcc tggcctgcca caagggctcg ctgctgggcg
     4321 aactccccgg cgtgccgcag ccctcgcaga aggcgttcga gaccggcctc gtcgacgcgc
     4381 tcgagcgcct cgaccccgcg cgtccggtgt tcgtcgaggg cgagagccgg cgcatcggcc
     4441 gcctgcaggt gccgctcgcg ctagtgcagc gcatgcgcgc ggcgccggtg gtcgagatcg
     4501 tcgcgccgtt ccaggcccgc gtcgactacc tgctgcgcga ttacgcctac ctcggcgatc
     4561 gccccgactg gttcgccggc cagctgcgcg cgctcaccga gctgcacggc aaggagacca
     4621 tcgcggcctg gcaggcgatg gcgcaggcgc acgagctcgc gccgctgttc gccgagttgg
     4681 ccgggcgcca ttacgacccg gcctacgcgc gctcgcaggg cgtgcacttc gcgcgctggg
     4741 aggcgcgcac ccgggtcgag gcgccggggc tcgaggacgc ggacatcgat gcgctcgcgg
     4801 cacgcatcgc ggcgctgccc gcgacggatt gagccgaccg ccgcgccggg ccgccgccga
     4861 t
//
LOCUS       DASVLN010000921        11847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_371124, whole genome shotgun sequence.
ACCESSION   DASVLN010000921 DASVLN010000000
VERSION     DASVLN010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371124"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..777)
                     /locus_tag="VF453_21600"
     CDS             complement(31..777)
                     /locus_tag="VF453_21600"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEX7690316.1"
                     /translation="MTIPRLVARAPALALAALAACQCTHAADLAIMPVAVQLDAAHDR
                     TTVRVTNTGKDAVVLQADAIGWSRRSGVDIDSSTDDLIVNPPVFTVAPGATQIVRVGL
                     RRNSPSDHESTYRMVLREVPSPVDPADEAVQGQVRVLVALRVPVYVAPAVVRHEQRWI
                     AHYDVDGRLVAGVTNTGNVHYKIGTVRLASTQDSVATRLQGPESVVMPGEAREIALEP
                     PAAGQAGKPLALEVLTDRGLQHVSLDVASR"
     gene            complement(792..1310)
                     /locus_tag="VF453_21605"
     CDS             complement(792..1310)
                     /locus_tag="VF453_21605"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HEX7690317.1"
                     /translation="MKIRSGALLPLALAIAWAVLAWRPAHATTATGTFQVNATVVSAC
                     TVSGTTLSFGGAINPLSTGVPLDASSSLSVQCTNTTPYAVALNAGTHAGGPANFTARA
                     MASGSNSLGYQLYLDSGRSSVWGDGTGSSATSSGTGTGSTQTIPIYGRIPSLSGIVPG
                     TYTDTVTVTVTY"
     gene            complement(1510..2694)
                     /locus_tag="VF453_21610"
     CDS             complement(1510..2694)
                     /locus_tag="VF453_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX7690318.1"
                     /translation="MSHDDSGRKAGFETRVIHAGQFPDPSTGAIMPPIFQTSTYVQSS
                     PGVHKGYEYSRSQNPTREALERCVADLEGGRRGYAFASGLAGMSTVLELLDAGSHIVA
                     SDDLYGGSFRLFERVRKRSAGHEITFIDMSDPQAIRAAIRPNTRMLWVETPSNPLLKL
                     IDLAAVATIAREHGLIAVADNTFASPWGQNPLALGFDIVAHSTTKYLNGHSDVVGGIV
                     VVGAEQRQDEWAERLAFLHNAVGAIAGPFDSFLALRGVKTLALRMERHNRSALELAQW
                     LEAHPKVARVYYPGLESHPQHALAKRQMRGGGGMISMDLKGDLAGARRFLEAVHLFAL
                     AESLGGVESLIEHPAIMTHASIPPEHRAKLGIGDTLIRLSVGIEAIDDLKADLEAGFA
                     RV"
     gene            complement(2781..4181)
                     /locus_tag="VF453_21615"
     CDS             complement(2781..4181)
                     /locus_tag="VF453_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7690319.1"
                     /translation="MTSTAHPAADRPARPAVLDLIGNTPLVEVTRFDTGCCQLFLKLE
                     SQNPGGSIKDRIGLAMIETAERDGRLKPGGTVVEATAGNTGLGLALVARAKGYRVVLV
                     VPDKMSSEKVLHLKALGAEVHTTRSDVAKGHPDYYQDMAARLTKEIGDGAFYIDQFNN
                     PANPLAHETGTAPEIWQQTGHRVDAIVCGVGSGGTLTGLKRYFQRVSPATEFVLADPQ
                     GSVLADYIATGSIGTAGSWAVEGIGEDFVPSIADLSDVRTAYTIADQESFATARELLK
                     REGILAGSSTGTLLAAALRHCRAQTSPKRVVTFVCDTGTRYLSKVYNDSWMFDQGLLQ
                     RSSVGDLRDLISRRVQDEAVISVTPDDTLLTAFQRMRAADVSQLPVLQAGKLAGLLDE
                     SDLLLHVSADSERFRSPVSQAMNRQIRTLAPTAGLPALISTLGEGWVAVVADASGFHG
                     LITRFDLLNYLRKTLS"
     gene            4407..5246
                     /locus_tag="VF453_21620"
     CDS             4407..5246
                     /locus_tag="VF453_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HEX7690320.1"
                     /translation="MAMDVVDYEIKGSEMQFVEIELDPGEAAVGEAGSMMFMEAGITM
                     DTIFGDGSQQNSGLWGALVGAGKRLITGANIFTTVYANQAGQKRRVAFAAPVPGKIVA
                     IDLRQVGGSLVTQKQSFLCAARGVAIGLQFQQRLSAGFFGGDGFVMQRLDGDGMAFVH
                     AGGTVERRDLQFGETLLIEAGCLVAYTQGVSFEIQFVGGIKTALFGGEGLFLAKVTGP
                     GSVWIQSLPFSRLASRVFAAAPQNGGSSDRASGGSSGGVGVLGATAVGGILGGLGSLL
                     GDD"
     gene            5276..6202
                     /locus_tag="VF453_21625"
     CDS             5276..6202
                     /locus_tag="VF453_21625"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEX7690321.1"
                     /translation="MRLLLDSLRRAVGNCFLPRVLLLSLLPLLIAGAAELVWTRLFWE
                     ENVRGAREILTAIPGMAQAIGWVAVHVSDAFPSVVYAIVIIFLATPVFVAIALVLAAL
                     WMTPATASLVSRRRFPDLERRHGGSWFAGFARSMWHTLVALVLSLVTLPLWIIPPLAI
                     VLPTLIWGRATTRIMGYDGLAEHATVQEREAILRAHAWPLLMIGMATGLLSSTPTLLW
                     VISVPAAILFPIVMVGVTWIYTMIFAFSALWFTHYLLAALAGLRAAEAAALSVQPGAP
                     TASGALPLVEEVPAADRFSAPPPDGEDSFDAP"
     gene            6199..7014
                     /locus_tag="VF453_21630"
     CDS             6199..7014
                     /locus_tag="VF453_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX7690322.1"
                     /translation="MNTTTFGTIIVGDEILSGRREDRHLARVISALESRGLALSWARY
                     VGDEPARIAAEIRAAAEAGDAVFCFGGIGATPDDHTRQAAAAALGRPLALHPEAAVLI
                     ERRGRETAAEKGLAYDATGPEHQRRLQMGMFPDGATLIPNPYNRIPGFSVGRLHFMPG
                     FPVMAHPMLDWLLDTLYAGASAGGRALHAVRVRGTNESALIPVFEHVERAFPGVLTFS
                     LPSVDHPVFGPHIEIGVKGADGTRVEAAMAALRSGAAAAGAVEIAGGDGQAAV"
     gene            7275..8690
                     /gene="glnA"
                     /locus_tag="VF453_21635"
     CDS             7275..8690
                     /gene="glnA"
                     /locus_tag="VF453_21635"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX7690323.1"
                     /translation="MSKTVADVMKLVQENEVKFVDFRFTDTRGKEQHVSVPLSHFDED
                     KFTSGHAFDGSSIAGWKGIEASDMILMPDPNTANIDPFFEEPTLILTCDVIEPSDGKP
                     YERDPRSLAKRAEAYLKSSGIGDAAYFGPEPEFFVFDSVRWGVDMKGSFSEIESEEAA
                     WNTGKNYEHGNKGYRPTVKGGYFPVPPVDSGQDMRSEMCLLLEQMGVPVEVHHHEVAN
                     AGQMEIGTKFSTLVERADWLQLQKYVIHNVAFAYGKTATFMPKPIVGDNGSGMHVHQS
                     VWKDGKNLFAGEGYAGLSEFALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPVK
                     LAYSARNRSASIRIPFVANPKGRRVEARFPDPLMNPYLGFAALLMAGLDGVENKIHPG
                     EAATKDLYHLPPEEDAKIPTVCHSLDQALDYLDKDRAFLTKGGVFTDAYLDAYIELKM
                     QEVQRFRMTTHPVEFDMYYSL"
     gene            8896..9420
                     /locus_tag="VF453_21640"
     CDS             8896..9420
                     /locus_tag="VF453_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690324.1"
                     /translation="MTVASSSDFRRRAGVRALVAVSFAAAGLAVAPAHAEGSIYYVCP
                     GNVFTNTITAKEAEAKGCKAREAQQPTTIPAPPRRGATAVAAAPASSRVDAKEQQARD
                     SDARRILQDELAKAQAQLDTLNKEYNGGNPERQGDEKNYAKYQQRVADLKASIDRTQA
                     DIAAIQRELAKTAQ"
     gene            9595..10635
                     /gene="glnL"
                     /locus_tag="VF453_21645"
     CDS             9595..10635
                     /gene="glnL"
                     /locus_tag="VF453_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEX7690325.1"
                     /translation="MATLVAVVEPDGRCVFANSVLEATVGVARRKLTQGSIYDWFRES
                     AALAEAIAAVSRNEFSARRFDGSLRHAALTAPATGAALPVHVIVSQHDLHGGRVFVEM
                     IEIEQQTRQDREERALGQAQVTKELVRNLAHEIKNPLGGIRGAAQLLELEIDPALGEY
                     TQVIIHEADRLQVLVDRLLAPHRKAQVVADLNIHEVLERIRSLVLAEYPRGLRIERDY
                     DTSIPEFRGDREQLIQAVLNIVHNATQALAEQIETGDARIVLRTRVARGETIGKQRYR
                     LALELHIEDNGPGVPESIRDQIFYPLVSGREGGSGLGLTLAQTFVQQHLGTIACDSVP
                     GRTVFRILIPLH"
     gene            10653..>11847
                     /gene="ntrC"
                     /locus_tag="VF453_21650"
     CDS             10653..>11847
                     /gene="ntrC"
                     /locus_tag="VF453_21650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX7690326.1"
                     /translation="MKPIWIVDDDPSIRFVLEKALAREQLPVRSFASTREVLAALDTD
                     EPQVLVSDIRMPGGSGLDLLAKVKERLPGLPVIVMTAYSDLDSAVSSFQGGAFEYLPK
                     PFDLPRAVELIRRALEESLREEVLDERNTPAPEILGQAPAMQDVFRAIGRLSQSNVTV
                     LITGESGSGKELVARALHRHSPRGEGGSNGAFVAINTAAIPKDLLESELFGHERGAFT
                     GAQTMRRGRFEQADGGTLFLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHQPIKANVRV
                     IAATHQNLEERVRQGVFREDLFHRLNVIRLRLPPLRERAEDIPALARFFLQKSARELG
                     VEAKRLTDEALELMKSFDFPGNVRQLENICHWLTVMAPAQVIAPKDLPPELLTPRGSL
                     RATAAD"
BASE COUNT         1711 a         4126 c         4260 g         1750 t
ORIGIN      
        1 cggcgggcgg acgcgcgggc ggcccgggtg tcagcggctc gcgacgtcca gggagacatg
       61 ctgcaggccg cggtcggtga gcacctcgag cgcgagcggc ttgccggcct ggccggcggc
      121 gggcggctcg agcgcgatct cgcgcgcctc gcccggcatc acgaccgatt ccgggccctg
      181 tagccgcgtc gcgacggagt cctgcgtcga cgcgaggcgc acggtgccga tcttgtagtg
      241 cacgttgccg gtgttggtga ccccggcgac cagcctgccg tcgacgtcgt agtgcgcaat
      301 ccagcgctgc tcgtggcgca cgacggccgg cgcgacgtag accggcaccc gcagcgccac
      361 cagcacccgc acctggcctt gcacggcctc gtctgccgga tcgaccggcg agggcacctc
      421 gcgcagcacc atgcgatagg tcgactcatg gtccgacggg ctgttgcggc gcaggcccac
      481 gcgcacgatc tgcgtggcgc ccggcgcgac ggtgaagacg ggcgggttga cgatcaggtc
      541 gtcggtcgac gagtcgatgt cgacgccgga gcggcgcgac catccgatcg cgtccgcctg
      601 cagcacgacg gcgtccttgc cggtgttggt cacgcgcacg gtggttcggt cgtgcgcggc
      661 gtcgagctgc acggccaccg gcatgatggc caggtcggcg gcgtgcgtgc actggcacgc
      721 ggcgagcgcc gccagggcga gcgcgggcgc gcgggcaacg aggcgcggga tcgtcatggc
      781 gtcgtaccgc gtcagtaggt gacggtgacg gtgacggtgt cggtgtacgt acccggcacg
      841 atgccggaca gcgacgggat ccgcccgtag atcgggatcg tctgcgtgct gcccgtgccg
      901 gtgccgctgg aggtcgccga agagcccgtg ccgtcgcccc agacgctgga gcggccgctg
      961 tcgaggtaga gctggtagcc cagcgagttg ctgccgctgg ccatcgcgcg cgccgtgaag
     1021 ttcgccgggc cgcccgcgtg cgtgccggcg ttcagcgcga ccgcgtacgg cgtggtattg
     1081 gtgcactgca cgctgaggct ggagctagcg tcgagcggca cgccggtgct cagcgggttg
     1141 atcgcgccgc cgaagctgag ggtcgtgccg gagaccgtgc aggcgctgac gacggtcgcg
     1201 ttgacctgga aggtgccggt cgccgtggtg gcatgggccg gtcgccaggc caggacggcc
     1261 catgcaatgg cgagcgcgag gggcaggagc gcaccgctgc ggatcttcat gttgttgatc
     1321 tccgggcgag ccgcggcacg gtgtgcgcgt ccagctcgcc tgtcggagat atcgaactcc
     1381 gccgggcgcc gcgaataggg agcccatcca ttcgcggcgt gcggttggct cgacgggcgg
     1441 cgtcgggatt tccgacgccg ttgtggggaa ttgccgatag gcgtccgccg ctgcgcgcgg
     1501 cggggcggct cagacccgcg cgaagcccgc ctcgaggtcg gccttgaggt cgtcgatcgc
     1561 ctcgatgccc accgacagcc ggatcagcgt gtcgccgatg cccagcttcg cgcggtgctc
     1621 gggcgggatg gaggcgtggg tcatgatggc cggatgctcg atcaggctct cgacgccgcc
     1681 caggctctcg gcgagcgcga acaggtgcac ggcctcgagg aagcggcgcg cgccggcgag
     1741 gtcgcccttc aggtccatcg agatcatccc gccgccgccg cgcatctggc gcttcgcgag
     1801 cgcgtgctgc gggtggctct cgaggcccgg gtagtagacg cgcgccacct tgggatgcgc
     1861 ctccagccac tgcgcgagct cgagcgcgct gcggttgtgg cgctccatgc gcagcgcgag
     1921 cgtcttgacg ccgcgcagtg cgaggaacga gtcgaacggc ccggcgatgg cgcccaccgc
     1981 gttgtgcagg aaggcgaggc gctccgccca ctcgtcctgg cgctgctcgg cgcccacgac
     2041 cacgatgccg ccgacgacgt ccgaatgccc gttgaggtat ttcgtcgtcg agtgggcgac
     2101 gatgtcgaag ccgagcgcga gcgggttctg gccccacggg ctcgcgaagg tgttgtccgc
     2161 gaccgcgatc aggccgtgct cgcgcgcgat cgtcgcgacg gcggcgaggt cgatcagctt
     2221 gagcagcggg ttggacggcg tctcgaccca cagcatgcgc gtgttcggcc ggatcgccgc
     2281 gcggatcgcc tgcggatcgg acatgtcgat gaacgtgatc tcgtggcccg cgctgcgctt
     2341 cctcacgcgc tcgaacaggc ggaacgagcc gccgtagagg tcgtcgctcg cgacgatgtg
     2401 cgagccggcg tccagcagtt ccaggacggt cgacatcccg gccaggccgg aggcgaacgc
     2461 gtagccgcgc cggccgccct cgaggtcggc gacgcagcgc tcgagcgcct cgcgcgtggg
     2521 gttctggctg cgcgagtact cgtagccctt gtgcacgccg gggctggact gcacgtaggt
     2581 cgaggtctgg aagatcggcg gcatgatcgc gccggtcgac gggtcgggga actggcccgc
     2641 gtggatgacg cgggtctcga agccggcctt gcggccgctg tcgtcgtggc tcatggatgt
     2701 ccttcgtggc gtgcggtgcg cgcgctcgcg cgacctctga tgggatcgcg cgagcctagc
     2761 gcgtttcgcg ccgccgcggt tcagctcagg gtcttgcgca ggtagttgag caggtcgaag
     2821 cgggtgatca ggccgtggaa gccgctcgcg tcggcgacca ccgccaccca gccctcgccg
     2881 agcgtcgaga tcagcgcggg cagccccgcc gtcggggcga gcgtgcggat ctggcggttc
     2941 atcgcctggc tgaccgggct gcggaagcgc tcggagtcgg cgctgacatg cagcagcagg
     3001 tcggactcgt ccagcagtcc cgccagcttg cccgcctgca gcaccggcag ctgcgagacg
     3061 tcggccgccc gcatgcgctg gaacgcggtc agcagcgtgt cgtccggcgt gacgctgatg
     3121 acggcctcgt cctgcacgcg gcgcgagatc aggtcgcgca ggtcgccgac gctggatcgt
     3181 tgcagcaggc cctggtcgaa catccagctg tcgttgtaga ccttggacag gtaccgggta
     3241 cccgtatcgc acacgaacgt gacgacgcgc ttggggctgg tctgcgcgcg gcagtggcgc
     3301 agcgccgcgg ccagcagcgt gccggtcgac gagccggcga ggatgccctc gcgcttcagg
     3361 agctcgcgcg cggtggcgaa gctctcctgg tcggcgatcg tgtaggcggt gcgcacgtcc
     3421 gagaggtcgg cgatcgaggg cacgaagtcc tctccgatgc cctcgaccgc ccaggagccg
     3481 gcggtgccga tgctgccggt ggcgatgtag tccgcgagca ccgagccctg cgggtcggcg
     3541 aggacgaact cggtggccgg cgagacgcgc tggaagtagc gcttcaagcc ggtcagcgtg
     3601 ccgcccgagc cgacgccgca gacgatggcg tcgacgcggt ggccggtctg ctgccagatc
     3661 tcgggcgcgg tgccggtctc gtgcgcgagc gggttggcgg ggttgttgaa ctggtcgatg
     3721 tagaacgcgc cgtcgccgat ctccttcgtg aggcgggcgg ccatgtcctg gtagtagtcg
     3781 ggatggccct tggcgacgtc ggagcgcgtc gtgtgcacct cggcgccgag cgccttcagg
     3841 tgcagcacct tctccgagga catcttgtcg ggcaccacca gcaccacgcg gtagcccttg
     3901 gcgcgcgcga ccagggccag cccgagcccc gtgttgccgg ccgtcgcctc gaccaccgtg
     3961 ccgcccggct tcagccggcc gtcgcgctcg gcggtctcga tcatcgcgag gccgatgcga
     4021 tccttgatgg agccgcccgg gttctgcgac tcgagcttga ggaagagctg gcagcagccg
     4081 gtgtcgaagc gcgtgacctc gacgagcggc gtgttgccga tgaggtccag cacggcgggg
     4141 cgcgccggcc ggtccgcggc gggatgggcg gtggaggtca tgtctctcgt tccaggcggc
     4201 cccgagggct tgtggccggg ccgcgtcgtt gttgggggtg acgcaggata gccccgacct
     4261 ccgggcggtt ggcaagtgag aagcccgcat gggtttatgc acagtccgca gggcccgctc
     4321 gcggggcggg gacgcatcct gctttgaggg cagaatgcgc ggcacggcgc ttggcgcgcc
     4381 catcgactca agcgagggaa gacgacatgg ccatggacgt ggtggactac gagatcaagg
     4441 gctccgagat gcagttcgtc gagatcgagc tcgatcccgg cgaggccgcg gtgggcgagg
     4501 ccggcagcat gatgttcatg gaggccggca tcacgatgga caccatcttc ggcgacggct
     4561 cgcagcagaa ttccggcctg tggggcgcgc tggtgggcgc gggcaagcgc ctgatcacgg
     4621 gcgcgaacat cttcaccacc gtctacgcga accaggccgg ccagaagcgg cgcgtggcgt
     4681 tcgccgcgcc ggtgccgggc aagatcgtcg cgatcgacct gcgccaggtg ggcggctcgc
     4741 tcgtcacgca gaagcagtcc ttcctgtgcg cggcgcgcgg cgtcgcgatc gggctgcagt
     4801 tccagcagcg gctgtcggcc ggcttcttcg gcggcgacgg cttcgtgatg cagcgcctgg
     4861 acggcgacgg catggccttc gtgcacgcgg gcggcacggt cgagcggcgc gacctgcagt
     4921 tcggcgagac gctgctgatc gaggccggct gcctggtcgc gtacacgcag ggcgtgagct
     4981 tcgagatcca gttcgtcggc ggcatcaaga cggcgctgtt cggcggcgaa ggcctgttcc
     5041 ttgccaaggt cacggggccg ggcagcgttt ggatccagag cctgccgttc tcgcgcctgg
     5101 ccagccgcgt gttcgcggcc gcgccgcaga acggcggctc cagcgaccgc gcgagcggcg
     5161 gctcgtccgg cggcgtcggc gtgctcggcg cgacggcggt cggcggcatc ctcggcgggc
     5221 tcggcagcct gctcggcgac gactgaaggc ctccacgact cccgtccccg catccatgag
     5281 actgctcctc gactcgctgc gccgcgccgt cggcaactgc ttcctgccgc gggtgctcct
     5341 gctgtcgctg ctgccgctgc tgatcgccgg cgcggccgag ctcgtctgga cgcgcctgtt
     5401 ctgggaggag aacgtgcgcg gcgcgcgcga gatcctgacg gcgatcccgg gcatggccca
     5461 ggcgatcggc tgggtcgccg tgcacgtgag cgacgcgttc ccgagcgtgg tctacgcgat
     5521 cgtgatcatc ttcctcgcga cgcccgtgtt cgtggcgatc gcgctggtgc tggcggctct
     5581 gtggatgacc ccggcgaccg catcgctcgt gtcgcgccgg cgcttccccg acctcgagcg
     5641 ccgccatggc ggctcgtggt tcgcgggctt cgcgcgctcg atgtggcaca cgctggtcgc
     5701 gctcgtgctc tcgctcgtga cgctgccgct gtggatcatc ccgccgctgg cgatcgtgct
     5761 gcccacgctc atctggggcc gcgcgacgac gcgcatcatg ggctacgacg gcctcgccga
     5821 gcatgccacg gtgcaggagc gcgaggccat cctgcgcgcg cacgcctggc cgctgctgat
     5881 gatcggcatg gcgaccggtc tgctgagcag cacgccgacc ttgctgtggg tcatctccgt
     5941 gccggccgcg atcctgttcc cgatcgtgat ggtgggcgtc acgtggatct acacgatgat
     6001 cttcgcgttc tccgcgctgt ggttcaccca ctacctgctc gccgcgctgg ccggcctgcg
     6061 cgcggccgag gccgccgcgc tgtccgtgca gccgggcgcg ccgacggcat cgggagcgct
     6121 gcccctagtc gaggaagtgc ccgccgccga ccgtttctcc gccccgccgc cggacggcga
     6181 ggactccttc gacgcaccat gaacaccacg accttcggca cgatcatcgt cggcgacgag
     6241 atcctctcgg gccggcgcga ggacaggcat ctcgcccgcg tcatctccgc gctcgagtcg
     6301 cgcggcctcg cgctctcgtg ggcgcgctac gtcggcgacg agccggcgcg catcgccgcc
     6361 gagatccgcg ccgcggccga ggccggcgac gcggtcttct gcttcggagg catcggcgcg
     6421 acgcccgacg accacacgcg ccaggccgcg gcggcggcgc tggggcgccc gctggcgctg
     6481 catccggagg ccgcggtgct gatcgagcgc cgcggtcgcg agaccgcggc ggagaagggc
     6541 ctcgcctacg acgccaccgg ccccgagcac cagcgccgcc tgcagatggg catgttcccg
     6601 gacggcgcga cgctgatccc caacccgtac aaccgcatcc ccggcttctc ggtcggccgc
     6661 ctgcacttca tgccgggatt cccggtgatg gcgcacccga tgctcgactg gctgctggac
     6721 acgctctacg cgggcgcgtc cgccgggggc cgcgcgctgc acgcggtgcg ggtgcgcggc
     6781 accaacgagt ccgcgctgat ccccgtcttc gagcatgtcg agcgcgcgtt ccccggcgtt
     6841 ctgaccttca gcctgccgag cgtcgaccac cccgtcttcg ggccgcacat cgagatcggc
     6901 gtgaaaggcg cggacggcac gcgcgtcgag gcggcgatgg ccgcgctgcg cagcggcgcc
     6961 gcggccgccg gcgcggtcga gatcgccggt ggcgacggac aggccgcggt ctgagacaat
     7021 cgcccgcttc gccccgaccg ggggcacgga ggagtgcacc gtaaaggtgc ataaagcacc
     7081 ccaacaaggt gcgaaaatca ttcagcccag atttggtgcg cgcgtcctcg tccgccgcgc
     7141 ccgaaaaacc gccgcttccg tgccccattc ggcggcatgg aacctgcttt agctcttcag
     7201 caagccgctg aacccggctg gtgccccgtt cgccagcccc ggcctcgccg cctctacaac
     7261 ccaggagccc cttgatgtcc aagaccgtcg ccgacgtgat gaagttggtg caagagaacg
     7321 aagtcaagtt cgtcgacttc cgtttcaccg atacccgtgg caaggagcag cacgtctccg
     7381 tgccgctgtc ccacttcgat gaagacaagt tcacgtccgg ccacgccttc gacggctcgt
     7441 cgatcgccgg ctggaagggc atcgaggcct cggacatgat cctgatgccg gacccgaaca
     7501 cggccaacat cgacccgttc ttcgaagagc cgacgctgat cctgacctgc gacgtcatcg
     7561 agccgagcga cggcaagccc tacgagcgcg acccgcgctc gctggccaag cgcgccgagg
     7621 cctacctgaa gtcctcgggc atcggcgatg cggcgtactt cggccccgag ccggaattct
     7681 tcgtgttcga ctcggtgcgc tggggcgtgg acatgaaggg ctcgttctcg gagatcgagt
     7741 ccgaggaagc ggcctggaac acgggcaaga actacgagca cggcaacaag ggctaccgcc
     7801 cgaccgtcaa gggcggctac ttcccggtgc cgccggtcga ctccggccag gacatgcgct
     7861 cggagatgtg cctgctgctc gagcagatgg gcgtgcccgt cgaagtgcat caccatgaag
     7921 tggccaacgc cggccagatg gagatcggca cgaagttctc gacgctggtc gagcgcgccg
     7981 actggctgca gctgcagaag tacgtcatcc acaacgtcgc cttcgcctac ggcaagaccg
     8041 cgacgttcat gccgaagccc atcgtcggcg acaacggctc gggcatgcac gtgcaccagt
     8101 ccgtgtggaa ggacggcaag aacctgttcg ccggcgaagg ctatgccggc ctgtcggagt
     8161 tcgcgctgta ctacatcggc ggcatcatca agcacgcgcg cgccctcaac gcgatcacga
     8221 accccggcac gaacagctac aagcgcctgg tgcccggctt cgaggcgccg gtcaagctgg
     8281 cctactcggc gcgcaaccgc tcggcgtcga tccgcatccc gttcgtcgcc aacccgaagg
     8341 gccgccgcgt cgaggcgcgc ttcccggatc cgctgatgaa cccgtatctc ggcttcgcgg
     8401 cgctgctgat ggcgggcctg gacggcgtcg agaacaagat ccatccgggc gaggccgcga
     8461 cgaaggacct gtaccacctg ccgccggaag aggacgcgaa gatcccgacc gtctgccaca
     8521 gcctcgacca ggcgctcgac tacctcgaca aggaccgcgc gttcctcacc aagggcggcg
     8581 tgttcacgga cgcctacctg gacgcctaca tcgagctgaa gatgcaggag gtccagcgct
     8641 tccgcatgac gacgcacccg gtcgagttcg acatgtacta ctcgctgtga tggccgcccg
     8701 gccgcgagcg cggccggtgc catcggcgat tgcctcgaga agcggccccg cgacgggccg
     8761 cttttttcat ggcggcagga atgcccgggc ggcttttcga aggtttacga acggttgccg
     8821 ccgcggctgt cgtccgggcg gccgcctgcc gcgttggcgg atggacggcg cgccgatttg
     8881 cgccacaatg aaacgatgac cgtggcctcc tcctccgact tccgccgccg cgccggcgtt
     8941 cgcgcgctcg tcgccgtgtc gttcgccgcg gcgggccttg cggtcgcgcc ggcgcatgcc
     9001 gagggctcga tctactacgt gtgccccggc aacgtgttca cgaacacgat cacggccaag
     9061 gaggccgagg cgaagggctg caaggcccgg gaggcgcagc agcccacgac gatcccggcc
     9121 ccgccgcgcc gcggggccac ggccgtcgcc gccgcgcctg ccagcagccg cgtcgacgcc
     9181 aaggagcagc aggcgcgcga ctccgacgcc cgccgcatcc tgcaggacga gctcgccaag
     9241 gcgcaggccc agctcgacac cctcaacaag gaatacaacg gcggcaaccc cgagcgccag
     9301 ggcgacgaga agaactacgc gaagtaccag cagcgcgtcg ccgacctgaa ggcctcgatc
     9361 gatcgcacgc aggcggacat cgccgccatc cagcgcgagc tggcgaagac ggcgcagtag
     9421 ccgccgccgc cgccgccgcc gcccgtgccc gaccatcgcc gccgctccgc ccgccagggg
     9481 gggcacgcgt ccgccgtgcc ggtcgaggcg acggccgcga tcccgcccga cgaggccacc
     9541 gaggcatcgc ctgccgaggc cgtgggcggc atcgactatt ccgccttcga ccacctggcc
     9601 acgctggtcg cggtcgtcga gccggacggc cgctgcgtct tcgcgaactc cgtgctggag
     9661 gcgaccgtcg gcgtcgcgcg ccgcaagctc acgcagggca gcatctacga ctggttccgc
     9721 gagagcgccg cgctggccga ggcgatcgcg gcggtctcgc gcaacgagtt ctcggcgcgc
     9781 cgcttcgacg gctcgctgcg ccacgccgcg ctgaccgccc cggccaccgg cgcggcgctg
     9841 ccggtgcacg tgatcgtgag ccagcacgac ctgcacggcg gccgcgtgtt cgtcgagatg
     9901 atcgagatcg agcagcagac gcgccaggac cgcgaggagc gcgcgctggg ccaggcgcag
     9961 gtgaccaagg agctggtgcg caacctcgcg cacgagatca agaacccgct gggcggcatc
    10021 cgcggcgcgg cgcagctgct ggagctggag atcgatcccg cgctcggcga gtacacgcag
    10081 gtcatcatcc acgaggccga ccggctgcag gtgctggtcg accgcctgct cgcgccgcat
    10141 cgcaaggcgc aggtggtcgc cgacctcaac atccacgagg tgctggagcg catccgctcg
    10201 ctggtgctgg ccgaataccc gcgcgggctg cgcatcgagc gcgactacga cacgtcgatc
    10261 cccgagttcc gcggcgaccg cgagcagctg atccaggcgg tactcaacat cgtccacaac
    10321 gcgacgcagg cgctcgcgga gcagatcgag acgggcgacg cgcgcatcgt gctgcgcacg
    10381 cgggtcgcgc gcggtgaaac catcggcaag cagcggtacc gactggcact ggaattgcat
    10441 atcgaggaca acggcccggg cgtccccgag tccattcgcg atcagatctt ctatccgctg
    10501 gtgtcggggc gggaaggcgg gtcgggcctg ggcttgacgc tggcgcagac cttcgtccag
    10561 cagcacctcg gcacgatcgc ctgcgacagc gtcccaggac gcacggtttt caggatcctg
    10621 attccgctgc actgacctgc acggcgcacc gcatgaaacc catctggatc gttgacgacg
    10681 acccatccat ccgattcgtc ctcgagaagg ccctggcccg cgagcagttg ccggtgcgca
    10741 gcttcgcgtc gacgcgcgag gtgctcgcgg cgctcgacac cgacgagccg caggtgctgg
    10801 tcagcgacat ccggatgccg ggcggctccg gcctggacct gctcgccaag gtcaaggagc
    10861 gcctgccggg gctgccggtg atcgtgatga cggcgtactc cgacctggac agcgcggtct
    10921 cgtcgttcca gggcggcgcg ttcgagtacc tgcccaagcc cttcgacctg ccgcgggcgg
    10981 tggagctgat ccgccgggcg ctggaagaga gcctgcgcga ggaggtgctg gacgagcgca
    11041 acacgccggc gccggagatc ctcggccagg cgcccgcgat gcaggacgtc ttccgcgcga
    11101 tcggccggct gtcgcagagc aacgtgaccg tcctgatcac gggcgagtcc ggctcgggca
    11161 aggagctggt cgcgcgcgcg ctgcaccgcc acagcccgcg cggcgagggc ggcagcaacg
    11221 gcgcgttcgt cgccatcaac accgccgcca tcccgaagga cctgctggaa agcgagctct
    11281 tcggccacga gcgcggcgcg ttcaccggcg cgcagacgat gcgccgcggc cgcttcgagc
    11341 aggccgacgg cggcacgctg ttcctcgacg agatcggcga catgcccttc gacctgcaga
    11401 cgcgcctgct gcgcgtgctg tcggacggcc agttctaccg ggtcggcggc caccagccga
    11461 tcaaggccaa cgtgcgcgtg atcgccgcga cgcaccagaa cctcgaggag cgggtacggc
    11521 aaggggtatt ccgcgaggac ctgttccacc gcctcaacgt gatccgcctg cgcctgccgc
    11581 cgctgcgcga gcgggccgag gacatcccgg cgctcgcccg cttcttcctg cagaagagcg
    11641 cgcgcgagct gggagtcgag gccaagcgcc tcaccgacga ggcgctggag ctgatgaagt
    11701 cgttcgactt ccccggcaac gtgcgccagc tcgagaacat ctgccactgg ctgaccgtga
    11761 tggcgccggc gcaggtgatc gcgccgaagg acctgccgcc cgagctgctc acgccgcgcg
    11821 gcagcctgcg ggcgaccgcg gcggatg
//
LOCUS       DASVLN010000922         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_318982, whole genome shotgun sequence.
ACCESSION   DASVLN010000922 DASVLN010000000
VERSION     DASVLN010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318982"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VF453_21655"
     CDS             <1..1009
                     /locus_tag="VF453_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX7690327.1"
                     /translation="IVLAPPASALPDLARGQWLGLAELSIAVVLLLFAESWGAIRGAA
                     LRHGDAVDANRDLVALGVANLASGLLHGMPVGAGYSATSANEAAGATSRRAAWIAAAV
                     VLALVALLLPQIARIPHPVLAAVVIHAVSHTLSLDAFRPYFAWRRDRVVVLAAAAAVL
                     VLGVLNGLLAGIGVSIAMMLRGLSEPRVSELGRRGTGHDFLNLRLHPDASAIPGLLLV
                     RPEAPLFFANADRMLGDIRARIAAARPRGVVLSLEESPDLDGTTLEALRDFAAEQARG
                     GLRLVFARLKDPVLALLARTLAQSPALLEPGSVDDAVALLSPPAADAAAPPAHADPSS
                     D"
     gene            1075..>1565
                     /locus_tag="VF453_21660"
     CDS             1075..>1565
                     /locus_tag="VF453_21660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690328.1"
                     /translation="MALHSTPSLAGRRLATALAMLAVVALLLCASRAAALTRGTSPQG
                     WAFVEGGVGQGEVDDMKAELARYSLWILTAERTSGAHLSDVEVTILSDKGDKLFDTAL
                     DGPWLMVDLPLGRYEVRARAGQQTQHRITTIHPGDHHQIVFYFDVADESHPEVDDIRP
                     ASPP"
BASE COUNT          175 a          592 c          567 g          231 t
ORIGIN      
        1 gatcgtcctc gcgccgccgg cgtccgccct gcccgatctc gcgcgcgggc agtggctggg
       61 cctggccgag ctctcgatcg ccgtcgtgct gctgctgttc gccgagagct ggggcgcgat
      121 ccgcggcgcc gcgctgcgcc acggcgatgc cgtcgacgcc aaccgcgacc tcgtggcgct
      181 gggcgtcgcc aacctcgcct cgggcctgct gcacgggatg ccggtgggcg ccggctattc
      241 cgcgacctcc gccaacgagg ccgccggggc gaccagccgc cgcgcggcct ggatcgcggc
      301 ggcggtggtg ctcgcactcg tcgcgctgct gctgccgcag atcgcgcgca tcccccatcc
      361 cgtactcgcg gcggtggtga tccacgcggt gtcgcacacg ctgtcgctcg atgcgttccg
      421 cccctacttc gcgtggcggc gcgaccgcgt cgtcgtcctc gcggccgccg cggcggtgct
      481 ggtgctgggc gtgctgaacg gactgctcgc gggcatcggc gtcagcatcg cgatgatgct
      541 gcgcgggttg tccgagccgc gcgtgagcga gctcggccgc cgcggcacgg gccacgattt
      601 cctgaacctg cgcctgcatc ccgacgccag cgccatcccc ggcctgctgc tcgtgcgccc
      661 ggaggcgccg ctgttcttcg ccaacgccga ccgcatgctg ggcgacatcc gcgcccgcat
      721 cgccgccgcg cgcccgcgcg gcgtcgtgct gagtctcgag gaatcgcccg acctcgacgg
      781 cacgacgctg gaggccctgc gcgatttcgc ggccgaacag gcgcgcggcg ggttgcggct
      841 cgtgttcgcg cggctgaagg atccggtgct ggcccttctg gcgcggacgc tggcgcagtc
      901 gcccgcgctg ctggagccgg gcagcgtgga cgacgcggtg gcgctgctga gcccgccggc
      961 ggcggacgcg gccgcgccgc cggcccacgc cgatccctcg tcggattgac gccgctcaat
     1021 ccggccgtgt cgccgggcgc cgaggctgga ggccatcacg cgaaggatgt cgccatggcc
     1081 ctgcattcga caccgagtct tgccggtcgc cggctggcga cggcgctggc gatgctcgcc
     1141 gtggtcgcgc tgctgctgtg cgcctcgcgc gccgcggcgc tgacgcgcgg gacgtcgccg
     1201 cagggatggg cgttcgtcga gggcggcgtc ggccagggcg aggtcgacga catgaaggcc
     1261 gagctcgcgc gctacagcct gtggatcctc acggccgagc gcacgtccgg cgcccacctg
     1321 tccgacgtgg aggtcaccat cctgagcgac aagggcgaca agctcttcga caccgccctg
     1381 gacggcccct ggttgatggt cgaccttccg ctgggacgct acgaggtgcg cgcgcgcgcc
     1441 gggcaacaga cgcagcaccg gatcacgacg atccatccgg gcgaccatca tcagatcgtc
     1501 ttctacttcg acgtcgcgga cgagtcccac ccggaggtcg acgacatccg gcccgcgtcc
     1561 ccgcc
//
LOCUS       DASVLN010000923         6849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_534299, whole genome shotgun sequence.
ACCESSION   DASVLN010000923 DASVLN010000000
VERSION     DASVLN010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534299"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..3401
                     /locus_tag="VF453_21665"
     CDS             189..3401
                     /locus_tag="VF453_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7690329.1"
                     /translation="MSPSTPFIRRPVATSLLMLAIVLAGLVGFRFLPLSALPQVDYPT
                     IQVQTLYPGGSPEVMSRTITAPLEKQFGQMSGLARMGSTSAAGVSLVTLQFNLGLSLD
                     VAEQEVQAAINAGNSLLPADLPAPPVYAKVNPADAPVMTLAVSSRAMPLPDVQNLVNT
                     RLAQKISQVPGVGLVTLAGGERPAKRIQADTRAMASYGIDLSALRTAITNANANQAKG
                     SFDGASRAYSINANDQLLSVDDYRKLIVAYRNGAPVRLSDVAQVVDSAENLRLAAWAD
                     DTPAIIVNVQRQPGANVIQTVDLIKRQLPELTAALPASLDVRVLSDRTTGIRASVEHV
                     EFELVLAVVLVTLVIFAFLHSWRATVIASLSVPISLVGTCGAMYLLGYSLNNLSLMSL
                     TIATGFVVDDAIVMIENISRYIEEGDRPMQAALKGASQIGFTIVSLTVSLIAVLIPLL
                     FMGDIVGRLFREFAVTLAITILISAVVSLTLVPMMSGRMLRPEGTPHGFGRRFQRAFD
                     GIVARYDRSLTWVLDHQGLTLLVALATLALTVLLYVVIPKGLFPTQDTGQLQVRVQAS
                     QTVSFARMASLQRQVARALMTDPAVESIASIVGVDGANNTMLDTGSMLVNLKAGRSGA
                     QSDIMDRLRDLGQRVAGVALYLQPTQDLTIDSDTGPTQYRISLEGADEATVDQWAARL
                     VERLRTAPQVRNVVTDAGATGPAVEVDLDRDTASRLGISASTVDDALYSAFGQRIVST
                     IFTETNQYRVILEARPDEATTPESIGELRVLTTSGAPTPLSAFATVRTVQSPLQVTHV
                     AQYPATTIGFDTAAGTSLGTAVDSIRQAAADIHLPRSVSLVPLGAAGAYENSLSNQLW
                     LILAAVVCVYIVLGVLYESYVHPMTILSTLPSAGVGALLALLIGGQDLGVIGIIGIIL
                     LIGIVKKNAIMMIDFAIDAERGEGKSPREAIHQAALLRLRPILMTTFAALFAAIPLMF
                     GWGEGKELRHPLGLSIFGGLLVSQLLTLFTTPVIYLAFDRLGRRLRARFDLGKDDDGD
                     GDDEDEDDAPRALPAPAVRPALEGPDAPPLLEGPAGGGPG"
     gene            3398..6691
                     /locus_tag="VF453_21670"
     CDS             3398..6691
                     /locus_tag="VF453_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7690330.1"
                     /translation="MNLSSPFVRRPIATVLLTAGVALAGIAGFFALPVAPLPQVDYPA
                     ISVSASIPGASPDTMATSVATPLERRLGVISGVNEMTSNSGTGSTRVSLQFDLSRDID
                     GAAREVQAAINASRADLPATLKANPTYRKANPSDAPCIILALTSKTRSPQQIYDAVSN
                     VVQQKVAQVAGVGDVEIGGGSLPAVRIDLNPLALNKLGIPAEDVRAAIQASNATRPKG
                     SVEDDVRRLQIYTGQPGHTAAEYKPLVVAWRNGAAVHLSDVADVTDGPEDVRTLGLFN
                     GMPAVIVIVTRQPGANIIDTVDGVRALIPELKEQLPNDIDLQVASDSTNSIRASLHEV
                     EATLVIAIALVVAIVGLFLHSLRATLVPGIATVVSLLGTFGVMFLLHFSLDNLSLMAL
                     TVATGFVVDDAIVVLENTSRHIEQGMDRFSAALLGAREVGFTVLSISLSLIAVFIPLL
                     FMGGQAGRLFREFAVTLSAAVMISLVISLTTTPMMCAWLLDRNKDRRRKARRPRGRFA
                     RITEGAFERVTRAYAHALDWALDAPVSVMVVLAAVVALNVWLFVAVPKGFFPMQDSGQ
                     LNGGMRADQSVSFDEMQSKLRQMVDIIRADPAVDTVVGFTGGARAGGGFMFVNLKPRG
                     IGQGKRREGSLAVIARLRPQLARVTGIQLFLNPVQDLRMGGRQTNSTYQYTLKADNQA
                     DLSTWATRLAEALRNDPELTDVDTDQQENGVEAMVDIDRDALARQGMSNRDVDNALYD
                     AFGQRQVATIYDELNQYHVVMENGPRFGRTPLALRDVYVAGNGANAAPPAGAASAAYS
                     AASTAASANPGLRDASTGATVNTSAHTMIPLSTIATFTEDAARASVNHQDGELATTVS
                     YNLADGVSLSQARDVIEREVAAIHMPVNVRGSFQGTAAAAQQNENQQPLLILAAIVVI
                     YIVLGMLYESLVHPVTVLSTLPSAGVGAVLALMLFGMEFSLIALIGVFLLIGIVKKNA
                     IMIIDFALEAERARGLSSRDAVREACMLRFRPILMTTLAAALGALPLAIGFGEGSELR
                     QPLGIAIIGGLVASQMLTLLTTPVVYLLLDKLRTRSPDEKTLSRHAVLDEPDAAAGTG
                     SPPHAATTP"
BASE COUNT          984 a         2539 c         2337 g          989 t
ORIGIN      
        1 cgccgcagga cgccgcctcg catgccggcg tcggggcgcg cggcgcatcc tggcccgcca
       61 gcggcgcttc gggcgcgtgg gggcaccatc gcggcgccag cggggtcgac cgcacctcgt
      121 gacgcccgcc gcgaccgcat cgtccctgcc gccacgaccc cgatcctgaa cccggccccg
      181 cccgagagat gagcccgtcc acccccttca tccggcgacc ggtcgcgacc tcgctgctga
      241 tgctcgcgat cgtgctggcc ggcctcgtcg gcttccgctt cctgccgctg tcggcgttgc
      301 cgcaggtcga ctacccgacg atccaggtgc agacgctcta ccccggcggc agccccgagg
      361 tcatgagccg gacgatcacc gcgccgctgg aaaagcagtt cggccagatg tcgggcctcg
      421 cgcgcatggg ctcgacgagc gcggcgggcg tctcgctcgt cacgctgcag ttcaacctcg
      481 gcctgtcgct ggacgtcgcg gagcaggaag tgcaggccgc gatcaacgcc ggcaactcgc
      541 tgctgcccgc ggacctgccc gcgccgccgg tctacgccaa ggtgaacccg gccgacgcgc
      601 ccgtgatgac gctggccgtg agctcccggg cgatgccgct gcccgacgtg cagaacctcg
      661 tgaacacgcg gctcgcgcag aagatcagcc aggtgccggg cgtgggcctc gtcacgctgg
      721 ccggcggcga gcgtccggcc aagcgcatcc aggccgacac gcgggcgatg gcctcgtacg
      781 gcatcgacct gtcggcgctg cgcaccgcga tcaccaacgc caacgccaac caggccaagg
      841 gcagcttcga cggcgcgtcg cgcgcgtact cgatcaacgc caacgaccag ctgctgtccg
      901 tcgatgacta caggaagctg atcgtcgcct accgcaacgg cgcgcccgtg cggctgtccg
      961 acgtcgcgca ggtcgtcgac agcgccgaga acctgcgcct ggccgcctgg gccgacgaca
     1021 cgccggccat catcgtcaac gtccagcgcc agcccggcgc caacgtgatc cagacggtcg
     1081 acctgatcaa gaggcagttg cccgagctca ccgccgcgct gcccgcgtcg ctggacgttc
     1141 gcgtgctgag cgaccgcacc accggcatcc gcgcttcggt ggagcacgtc gagttcgagc
     1201 tcgtgctcgc cgtggtcctg gtgacgctgg tgatcttcgc gttcctgcac agctggcgcg
     1261 cgacggtgat cgcgagcctg tcggtgccga tctccctcgt gggcacctgc ggcgcgatgt
     1321 acctgctggg atacagcctc aacaacctga gcctgatgtc gctcacgatc gccaccggct
     1381 tcgtcgtcga cgacgcaatc gtgatgatcg agaacatctc gcgctacatc gaggaaggcg
     1441 accggccgat gcaggccgcg ctcaagggcg cgtcgcagat cggcttcacg atcgtgtcgc
     1501 tgacggtctc gctgatcgcg gtgctgatcc cgctgctgtt catgggcgac atcgtcggcc
     1561 gcctgttccg cgagttcgcc gtgacgctcg cgatcacgat cctgatctcg gcggtggtct
     1621 cgctgaccct ggtgccgatg atgtcgggcc gcatgctgcg ccccgagggc acgccgcacg
     1681 gcttcgggcg ccggttccag cgcgcgttcg acggcatcgt cgcgcgctac gaccgctcgc
     1741 tgacgtgggt gctcgaccac cagggcctga ccctgttggt cgcgctcgcg acgctcgcgc
     1801 tgaccgtcct gctgtacgtc gtgattccca agggcctgtt ccccacccag gacaccggcc
     1861 agctgcaggt gcgcgtgcag gcctcgcaga ccgtgagctt cgcgcgcatg gcctcgctgc
     1921 agcggcaggt cgcgcgggcg ctgatgaccg atccggccgt cgagagcatc gcctcgatcg
     1981 tcggcgtcga cggcgccaac aacacgatgc tggacaccgg cagcatgctc gtcaacctga
     2041 aggccgggcg ctcgggcgcg cagtcggaca tcatggaccg gctgcgcgac ctcggccagc
     2101 gcgtggcggg cgtcgcgctg tacctgcagc cgacgcagga cctgacgatc gactccgaca
     2161 ccggccccac gcagtaccgc atctcgctcg agggcgccga cgaggcgacg gtcgaccagt
     2221 gggcggccag gctggtcgag cgcctgcgca cggccccgca ggtgcgcaac gtggtcaccg
     2281 acgccggcgc gaccgggccg gcggtggagg tcgacctcga ccgcgacacc gcctcgcgcc
     2341 tgggcatcag cgcctcgacg gtggacgacg cgctctacag cgccttcggc cagcgcatcg
     2401 tctcgacgat cttcaccgag accaaccagt accgcgtgat cctcgaggcg cggcccgacg
     2461 aggccaccac gcccgagtcg atcggcgagc tgcgcgtgct gaccaccagc ggcgcgccga
     2521 cgccgctgtc ggccttcgcc accgtgcgca ccgtgcagtc gccgctgcag gtcacgcacg
     2581 tcgcgcagta tcccgcgacg accatcggct tcgacacggc ggcaggcacc tcgctcggca
     2641 cggcggtcga ctcgatccgc caggccgccg ccgacatcca cctgccccgg agcgtgtcgc
     2701 tcgtgccgct gggcgcggcg ggcgcgtacg agaactcgct gtccaaccag ctgtggctga
     2761 tcctggcggc cgtggtctgc gtctacatcg tgctgggcgt gctctacgag agctacgtgc
     2821 atccgatgac catcctgtcg accctgccct cggccggcgt gggcgcgctg ctcgcgctgc
     2881 tgatcggcgg ccaggacctg ggcgtgatcg gcatcatcgg catcatcctg ctgatcggca
     2941 tcgtcaagaa gaacgccatc atgatgatcg acttcgccat cgacgccgag cgcggcgagg
     3001 gcaagtcgcc gcgcgaggcg atccaccagg cggcgctgct gcgcctgcgg cccatcctga
     3061 tgacgacctt cgccgccctg ttcgccgcca tcccgctgat gttcggctgg ggcgagggca
     3121 aggaattgcg ccacccgctg ggcctgtcga tcttcggcgg cctgctcgtg agccagctgc
     3181 tcaccctctt cacgacgccg gtgatctacc tcgcgttcga ccgcctcggg cggcgcctgc
     3241 gcgcccgctt cgacctgggc aaggacgacg acggcgacgg cgacgacgag gacgaggacg
     3301 acgcgccgcg cgctctgccc gcgccggcgg tacggccggc gctcgaaggc ccggacgcgc
     3361 cgccgctgct cgaaggcccc gccggcgggg ggccgggatg aacctgtcct cgcccttcgt
     3421 ccggcggccg atcgccacgg tgctgctgac cgccggcgtc gcgctggccg gcatcgcggg
     3481 cttcttcgcg ctgccggtgg cgccgctgcc gcaggtggac tatccggcga tctcggtgtc
     3541 cgcgagcatc cccggcgcga gcccggacac gatggccacg agcgtcgcca cgccgctgga
     3601 gcgccggctg ggcgtgatct cgggcgtcaa cgagatgacg tccaacagcg gcaccggctc
     3661 gacgcgcgtg agcctgcagt tcgacctcag ccgcgacatc gacggtgccg cgcgcgaggt
     3721 gcaggcggcg atcaacgcct cgcgcgccga cctgcccgcg acgctgaagg ccaatccgac
     3781 ctaccgcaag gccaacccgt cggacgcgcc ctgcatcatc ctcgcgctga cctcgaagac
     3841 gcgctcgccg cagcagatct acgacgcggt ctccaacgtc gtgcagcaga aggtcgcgca
     3901 ggtcgcgggc gtgggcgacg tcgagatcgg cggcggctcg ctgccggccg tgcgcatcga
     3961 cctgaacccg ctcgcgctga acaagctggg catcccggcg gaggacgtgc gcgccgcgat
     4021 ccaggcctcc aacgcgacgc gccccaaggg cagcgtggag gacgacgtcc ggcgcctgca
     4081 gatctacacc ggccagcccg gccacacggc ggccgagtac aagccgctgg tcgtcgcctg
     4141 gcgcaacggc gcggcggtgc acctgtcgga cgtggccgac gtgaccgacg gaccggagga
     4201 cgtgcgcacg ctgggcctgt tcaacggcat gcccgccgtg atcgtgatcg tcacgcgcca
     4261 gccgggcgcc aacatcatcg acaccgtcga cggcgtgcgc gcgctgatcc cggagctgaa
     4321 ggagcagctg cccaacgaca tcgacctgca ggtcgcctcg gactcgacca actcgatccg
     4381 cgcctcgctg cacgaggtcg aggcgacgct cgtcatcgcg atcgcgctgg tcgtggcgat
     4441 cgtcggcctg ttcctgcaca gcctgcgcgc gacgctggtg ccggggatcg cgacggtggt
     4501 ctctctgctg ggcaccttcg gcgtgatgtt cctgctgcac ttcagcctcg acaacctgag
     4561 cctgatggcg ctgacggtgg ccaccggctt cgtggtcgac gacgcgatcg tcgtgctgga
     4621 gaacaccagc cgccacatcg agcagggcat ggaccgcttc agcgccgcgc tgctgggcgc
     4681 cagggaggtc ggcttcacgg tgctgtcgat cagcctgtcg ctgatcgccg tgttcatccc
     4741 gctgctgttc atgggcgggc aggcggggcg gctgttccgc gagttcgcgg tcacgctgtc
     4801 ggcggccgtg atgatctcgc tcgtgatctc gctgacgacg acgccgatga tgtgcgcgtg
     4861 gctgctcgat cgcaacaagg acaggcggcg caaggcccgg cggccgcgcg gccgcttcgc
     4921 gcgcatcacc gagggcgcct tcgagcgcgt gacgcgcgcc tacgcgcacg cgctggactg
     4981 ggcgctggac gcgccggtct ccgtgatggt ggtgctggcg gcggtggtgg cgctcaacgt
     5041 gtggctgttc gtggccgtgc ccaagggctt cttcccgatg caggactccg gccagctcaa
     5101 tggcggcatg cgtgccgacc agagcgtctc cttcgacgag atgcagtcca agctgcgcca
     5161 gatggtcgac atcatccgcg ccgacccggc ggtcgacacg gtggtcggct tcaccggcgg
     5221 cgcgcgcgcg ggcggcggct tcatgttcgt caacctgaag ccgcggggca tcggccaggg
     5281 caagcgcagg gaaggcagct tggcggtgat cgcgcgcctg cgtccgcaac tggccagggt
     5341 gacgggcatc cagctgttcc tcaacccggt gcaggacctg cgcatgggcg ggcggcagac
     5401 gaactcgacc taccagtaca cgctcaaggc cgacaaccag gcggaccttt cgacctgggc
     5461 gacccggctt gccgaggcgc tcaggaacga ccccgagctc accgacgtcg acaccgacca
     5521 gcaggagaac ggcgtcgagg ccatggtcga catcgaccgc gacgccctcg cccgccaggg
     5581 catgagcaac agggacgtcg acaacgcctt gtacgacgcg ttcggccagc gccaggtcgc
     5641 gacgatctac gacgagctga accagtacca cgtcgtgatg gagaacgggc cgcgcttcgg
     5701 ccgcacaccc ctggcgctgc gcgacgtcta cgtggcgggc aacggcgcga acgcggcgcc
     5761 gccggcgggt gccgcgagcg ccgcctactc cgcggccagc accgccgcct ccgccaaccc
     5821 ggggctgcgc gacgcctcca ccggcgcgac ggtcaacacc agcgcgcaca cgatgatccc
     5881 gctgtcgacg atcgccacgt tcacggagga cgccgcgcgc gcgagcgtca accaccagga
     5941 cggcgagctc gcgaccacgg tctcgtacaa cctggccgac ggcgtctcgc tgtcccaggc
     6001 gcgcgacgtg atcgagcgcg aggtggccgc gatccacatg ccggtcaacg tgcgcggcag
     6061 cttccagggc accgcggcgg ccgcgcagca aaacgagaac cagcagccgc tgctgatcct
     6121 cgcggccatc gtcgtgatct acatcgtgct gggcatgctc tacgagagcc tcgtgcatcc
     6181 ggtgaccgtg ctgtcgacgc tgccctcggc gggcgtgggc gccgtgctcg ccctgatgct
     6241 gttcggcatg gagttctcgc tgatcgcgct gatcggcgtc ttcctgctga tcggcatcgt
     6301 caagaagaac gcgatcatga tcatcgactt cgccctcgag gccgagcgcg cgcgcgggct
     6361 gtcctcgcgc gacgccgtgc gcgaggcctg catgctgcgc ttccggccga tcctgatgac
     6421 gacgctggcc gccgcgctcg gcgcgctgcc cctggcgatc ggcttcggcg agggctccga
     6481 gctgcgccag ccgctgggca tcgcgatcat cggcggcctg gtcgcgagcc agatgctgac
     6541 gctgctgacc acgccggtcg tctacctgct gctggacaag ctgcgcaccc gcagccccga
     6601 cgagaagacc ctgagccgcc acgcggtcct cgacgaaccg gatgccgccg ccggcaccgg
     6661 gtcgccgccg cacgccgcca ccacgccatg aaccgatacg ccgcttccct cgcccatccc
     6721 gcccggcgcg gcgcgcgccc gcgcgtgaac gccctggccg tcgcggtggc catcgcggcc
     6781 gcgctgggcg gctgcgccac ccacggcccg gacgcgccgc ccgaggcccc ggccgcgccc
     6841 gcatggctc
//
LOCUS       DASVLN010000924         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_209660, whole genome shotgun sequence.
ACCESSION   DASVLN010000924 DASVLN010000000
VERSION     DASVLN010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209660"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VF453_21675"
     CDS             complement(<1..1035)
                     /locus_tag="VF453_21675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7690331.1"
                     /translation="MRSLFLLNSLCFGGAEKQVVALVNGLCQQNGHRVWIQCLKDDDA
                     LLPQVDPALRPQVLPNLHVRSGLEPFAVAALARRIDAHAIDVVVCTNMYALLYGSLAR
                     ALCRRRNALRLVEVFHTTEVASRKERLSMTLYRRLLRATDLLVYVCRGQAAHWQAQGL
                     RAREEVVIHNGIDTARFTDRSTPAENEALRRSFGFSPEDFVVGICAVMRPEKAHDDLV
                     RAVAALHARGVPVRALLVGDGPLRGAVEARIAELGLTASIRITGLVADVRPYVAACDA
                     MALTSRTETFSLAALEAMAMGKPMVMARIGGAAEQVDDGVDGLLYPPGDVAALTDALG
                     AMADRARCAAM"
     gene            complement(1149..>2703)
                     /locus_tag="VF453_21680"
     CDS             complement(1149..>2703)
                     /locus_tag="VF453_21680"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7690332.1"
                     /translation="MAFIVLGALVPLPLILRDYRTGVAMLAAMLPIASMLPSVKGLNP
                     LNFVTAATLVSLFLRDAFSSRRAVRLPGVLWAGLVLPATWAIVVAWPHIPEGVHNYPL
                     LDNAREIYDPWSYVAARYAKPLFYYLSFAMLVANAARDSRRPERFALLLAAAMVLPSL
                     AVFVTVHQYPGTLADVSRDREFMAPRGMHANEFGMLLAAGSGPLLFGAAGAGGRTARW
                     TLRAAFALVTLALVLTFSRGGLTAWLVVVGGYLVQHRRLKTFLVAATVGALALAAAPQ
                     SVTERFGAGMHEGAISDVSDVGKDELTAGRVHGWQLLAPEVLDSPWFGRGLGSTQWSQ
                     AVEAGRYKANHPHNIYLELLMDVGLVGFAAMAFLHWTLLRRLRALAADASLGPALRGF
                     FAGARWSLLGVLVMAATTAYYMPNSAQFPLWFCFGMAFSVAPSIPRAASALRALNDAR
                     GPAATMVAAARDSGRQPQRGGPHEQAPGLAHDVDGAGRGRRLASPPGVRRSADRPAGG
                     RARPCTGQG"
BASE COUNT          417 a          951 c         1025 g          310 t
ORIGIN      
        1 catcgccgcg cagcgcgcgc ggtcggccat cgccccgagc gcgtccgtca gcgccgcgac
       61 gtcgccgggc ggatacagca ggccgtcgac gccgtcgtcg acctgctccg ccgcgccgcc
      121 gatgcgggcc atcaccatcg gcttgcccat ggccatcgcc tccagcgcgg ccagcgagaa
      181 ggtctcggtg cgggaggtca gcgccatcgc gtcgcaggcg gccacatacg gacgcacgtc
      241 cgcgacgagc cccgtgatcc tgatcgacgc ggtcaggccg agctcggcga tgcgcgcctc
      301 gacggcaccg cgcagcggcc cgtcgccgac cagcagggcg cgcaccggca cgccgcgggc
      361 atgcagcgcg gccaccgcgc gcaccaggtc gtcgtgcgcc ttctcgggcc gcatgaccgc
      421 gcagatgccg acgacgaagt cctccggcga gaaaccgaag gatcggcgca gcgcctcgtt
      481 ctcggccggc gtggagcggt cggtgaagcg cgcggtgtcg atgccgttgt ggatcacgac
      541 ctcctcgcgc gcgcgcaggc cctgcgcctg ccagtgggcg gcctggccgc ggcagacgta
      601 gaccagcagg tccgtcgcgc gcagcaggcg gcggtacagc gtcatcgaca ggcgctcctt
      661 gcgcgaggcg acctcggtcg tgtggaagac ctccacgagc cgcagcgcgt tgcggcggcg
      721 gcacaacgcg cgcgccaggc tgccgtacag cagcgcgtac atgttggtgc agaccacgac
      781 gtcgatggcg tgcgcgtcga tgcggcgcgc gagggcggcg accgcgaagg gctcgagccc
      841 ggatcgcacg tgcaggttgg gcagcacctg cggccgcagc gccgggtcga cctgcggcag
      901 cagcgcgtcg tcgtccttca ggcactggat ccacacgcga tggccgttct gctggcacag
      961 gccgttgacg agggcgacga cctgcttctc ggcgcccccg aagcacagcg agttgagcag
     1021 gaaaaggctt cgcacggcgg cgggggcggc ggtggggtgg gatggtgtca tctcaatggc
     1081 gttggcggta ggcgacgagt gcgagggcgg cggtgccggc caggaccagc gtccaggttc
     1141 ccggcccgtc atccttgtcc ggtgcagggg cgggcgcggc cgccggcagg ccggtcggcg
     1201 cttcggcgaa cgccgggcgg agatgccagg cgccggccgc gcccagcgcc gtccacatcg
     1261 tgagcgagac caggagcttg ttcatggggc cctcctcgtt gcggttggcg gccgctgtca
     1321 cgggccgctg caaccatcgt ggccgcgggg ccgcgggcgt cgttgagggc acgcaatgcc
     1381 gatgcggcgc ggggtatcga gggcgcgacc gagaacgcca tgccgaagca gaaccacagc
     1441 gggaactgcg ccgagttggg catgtagtag gccgtcgtcg cggccatcac gagcacgccc
     1501 aggagcgacc agcgcgcgcc cgcgaagaag ccgcgcagcg caggcccgag cgacgcgtcc
     1561 gccgcgagcg cgcgcagccg ccgcagcagc gtccagtgca ggaaggccat cgccgcgaag
     1621 ccgacgaggc cgacgtccat cagcagctcg aggtagatgt tgtgcggatg gttggccttg
     1681 tagcggcccg cctccaccgc ctgggaccac tgcgtcgagc cgaggccgcg gccgaaccag
     1741 gggctgtcca ggacctcggg cgcgagcagc tgccagccgt gcacgcgtcc ggccgtgagc
     1801 tcgtccttgc cgacgtcgga gacgtccgag atcgcaccct cgtgcatgcc ggcgccgaag
     1861 cgttcggtga cggactgggg cgccgcggcc agcgcgagcg cgcccaccgt ggcggcgacg
     1921 agaaaggtct tgagccggcg gtgctgcacg aggtagccgc cgacgacgac gagccaggcc
     1981 gtgagcccgc cgcgcgagaa cgtcagcacc agcgccagcg tgaccagcgc gaacgccgcg
     2041 cgcagcgtcc agcgcgcggt gcgccccccg gcgcccgccg cgccgaacag caagggcccc
     2101 gacccggccg ccagcagcat gccgaactcg ttggcgtgca tgccgcgcgg cgccatgaac
     2161 tcgcggtcgc gcgagacgtc cgcgagcgtg cccggatact ggtgcacggt gacgaacacg
     2221 gccagcgagg gcagcaccat tgccgcggcc agcagcagcg cgaagcgctc ggggcggcgg
     2281 ctgtcgcgcg cggcgttggc cacgagcatc gcgaacgaca ggtagtagaa cagcggcttg
     2341 gcgtagcgcg ccgcgacgta ggaccacggg tcgtagatct cgcgggcgtt gtccagcagc
     2401 gggtagttgt gcacgccctc ggggatgtgc ggccacgcga cgacgatggc ccaggtcgcg
     2461 ggcagcacga ggccggccca gagcacgccc ggcagcctca ccgcgcgccg cgacgagaat
     2521 gcgtcgcgca ggaacagcga cacgagcgtc gcggccgtga cgaagttcag ggggttcagg
     2581 cccttgacgg acggcagcat ggacgcgatg ggcagcatcg cggccagcat cgccacgccc
     2641 gtgcggtagt cgcgcaggat gagcggcagc ggcaccagcg cgccgagcac gatgaaggcc
     2701 atc
//
LOCUS       DASVLN010000925         4796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_103392, whole genome shotgun sequence.
ACCESSION   DASVLN010000925 DASVLN010000000
VERSION     DASVLN010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103392"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..3959
                     /gene="mfd"
                     /locus_tag="VF453_21685"
     CDS             330..3959
                     /gene="mfd"
                     /locus_tag="VF453_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX7690333.1"
                     /translation="MQLPSPVPGQRYTLPRPPGAADALFVAHLAAERATRGQLTLVVC
                     AEPADAQRLADELPFFAPHLRIAVFPDWETLPYDQFSPHQDLVSERLATLWRLYRKRE
                     GLSSVNAGAGAALPKSPKAAADPTKAAAAKADEDDGVDVVLLPASTALTRLAPPSFLA
                     ATTFHFKQKTKLDEAGLRAQLALAGYSHVSSVVSPGEYAVRGGLIDLFPMGSAVPYRV
                     DLFDNEVDSIRTFDPDSQRSLYPVPEVRLLPGREFPLDEAARKAFRVRWRERIEGDPT
                     KARLYKDMGNGIAGGGIEYYLPLFFDETATIFDYLPASAQLVLHGGVDEALQRFWTDT
                     RERYRFQRADPERPILPPEDVFLTSEQFFGATRAFAALSLRPPGAAPTGQAGAEGEAV
                     PSAVPWARPLPDVSVDRGATAPLAKLQRHLDTTPHRVLLVADSEGRRETLLDLLRDHG
                     IEPPSVATLAEFEAGDEKVAITAAPIGEGFFWFEPEAQGRPRAIQFITENELFATGAT
                     PRRRRRGQEQTSSVDALIKDLSELKQGDPVVHQQHGIGRYCGLVNIDLGNGEGGPSEF
                     LHLEYADKATLYVPVAQLALISRYTGVSAEEAPLHKLGSGQWEKARRKAAEQVRDAAA
                     ELLNLYARRAARDGHSHRFSPHDYEAFATSFGFEETPDQRAAIHAVIQDMVSPKPMDR
                     LVCGDVGFGKTEVALRAAFVAVHGGKQVALLAPTTLLAEQHYQTISARFGQWPVKVAE
                     MSRFRTAKEIAAAMAGLADGTIDIVVGTHKLLSQDVKFKRLGLLIIDEEHRFGVRHKE
                     AMKALRAEVDVLTLTATPIPRTLGMALEGLRDLSVIATAPQRRLAIKTFVRSESQGTI
                     REAVLRELKRGGQVYFLHNEVETIANRRDKLAELLPEARIAIAHGQMPERELEAVMRD
                     FVAQRVNVLLCSTIIETGIDVPTANTIVISRADKFGLAQLHQLRGRVGRSHHQAYAYL
                     LVPDVEGLTKQAAQRLDAIQAMEELGSGFYLAMHDLEIRGAGEVLGESQSGNMQEVGF
                     QLYNDMLAEAVRALKSGQEPDLLSPTGAFGGGIDIKLHAPALLPDAYCGDVQTRLSLY
                     KRLATADRPEQLDALLEEITDRFGKLPPQGQALFDTHRLRILAKSYGVLKVDANPKVM
                     NITFRPNPPIDPLRIIELVQKNRVVKLAGNDRLRIDREIAEPRDRAQFVRDLLRSLGP
                     PKTPA"
     gene            complement(4207..4548)
                     /locus_tag="VF453_21690"
     CDS             complement(4207..4548)
                     /locus_tag="VF453_21690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690334.1"
                     /translation="MAQPIVKPVADPLDDIDRVQPVDSLMARQDRADLPGNRSDIPDP
                     DAPPPLGEGSPVIDPDDIPAPEGASTASLDDPEVAQEIENLVGDRPGLGPDDDEPIEE
                     TERLGDDSRTA"
BASE COUNT          699 a         1821 c         1647 g          629 t
ORIGIN      
        1 atctcgcctc cagcgtccac agcaccagcg cgcgcccggc gagcggcgcg tactgcttgg
       61 ggccgtcggc gcccgcgcgc tcgcccacgc cggcgcacgg cacgagcgcg aagcagcggg
      121 cgcgggcggg cgccgcgggc aggggaaagc gcgaatcacc gcgcatggaa cgggtcatgg
      181 ggccggattc tagaatccgc gcttgccccc gctcgaccgc gacgaccccg cgcctcgacg
      241 gcacgccgat cgccctgccc caagcgcagg ccgaccggcc tgtcgcaggc gcgccgtcgc
      301 ttcgacgccg ccgccctccc cgacccgaca tgcagctgcc ctcgcccgtc cccggccaac
      361 gctacaccct gcctcgcccg cccggcgcgg ccgacgcgct cttcgtcgcc cacctcgccg
      421 ccgagcgcgc gacgcggggg cagctcacgc tggtcgtgtg cgccgagccc gccgatgccc
      481 agcgcctcgc cgacgagctg cccttcttcg cgccgcacct gcgcatcgcc gtcttcccgg
      541 actgggagac gctgccctac gaccagttct cgccccacca ggatctcgtc tccgaacgcc
      601 tggccacgct ctggcgcctg taccgcaagc gagagggcct gagcagcgtc aacgcgggcg
      661 ccggtgcggc cctgcccaag tcccccaagg ccgccgccga tcccacgaaa gcggcggccg
      721 cgaaggccga cgaggacgac ggcgtcgacg tcgtgctgct gcccgccagc acggccctga
      781 cgcgcctcgc gcccccgtcc ttcctcgccg ccacgacgtt ccatttcaag cagaagacga
      841 agctcgacga ggcgggcctg cgcgcgcagc tcgcgctggc gggctactcg cacgtgagct
      901 cggtcgtctc gcccggcgag tacgcggtgc gcggcggcct gatcgacctg ttcccgatgg
      961 gctcggccgt gccctaccgg gtcgacctgt tcgacaacga agtcgactcc atccgcacct
     1021 tcgatcccga cagccagcgc agcctctacc ccgtgcccga ggtgcggctg ctgccggggc
     1081 gcgagttccc gctcgacgag gcggcgcgca aggcgttccg ggtgcgctgg cgcgagcgca
     1141 tcgagggcga cccgaccaag gcccggctct acaaggacat gggcaacggc atcgccggcg
     1201 gcggcatcga gtactacctg ccgctgttct tcgacgagac cgcgacgatc ttcgactacc
     1261 tgcccgcgag cgcgcagctc gtgctgcacg gcggcgtcga cgaggcgctg cagcgcttct
     1321 ggacggatac ccgggagcgg taccgcttcc agcgcgccga tcccgagcgg ccgatcctgc
     1381 cgcccgagga cgtcttcctc acgagcgagc agttcttcgg tgccacgcgc gcgttcgccg
     1441 ccctgtcgct tcgcccgcca ggcgccgcgc ccaccgggca ggccggcgcc gagggcgagg
     1501 ccgtgccgtc ggcggtgccc tgggcccgcc ccctgcccga cgtcagcgtg gaccgcggcg
     1561 ccaccgcccc gctcgcgaag cttcagcgcc acctcgacac gacaccgcac cgcgtgctcc
     1621 tggtcgccga cagcgagggc cggcgcgaga cgctgctgga cctgctgcgc gaccacggca
     1681 tcgagccgcc cagcgtcgcg acgctcgcgg agttcgaggc cggcgacgag aaggtggcga
     1741 tcacggcggc gccgatcggc gaaggcttct tctggttcga gcccgaggct cagggcaggc
     1801 cgcgcgcgat ccagttcatc accgagaacg agctcttcgc caccggcgcg acgccgcgcc
     1861 gccgccgccg cggtcaggag cagaccagca gcgtcgacgc gctgatcaag gatctctcgg
     1921 agctcaagca gggcgacccg gtcgtgcacc agcagcacgg catcgggcgc tattgcggcc
     1981 tcgtcaacat cgacctcggg aacggtgaag ggggcccgag cgagttcctg cacctggagt
     2041 acgccgacaa ggcgacgctg tacgtgccgg tcgcgcagct ggcgctgatc agccgctaca
     2101 ccggcgtctc ggccgaggag gcgccgctgc acaagctcgg ctccgggcag tgggagaaag
     2161 cgcgccgcaa ggccgccgag caggtgcgcg acgccgccgc cgagctgctg aacctgtacg
     2221 cccgccgcgc cgcgcgcgac ggccactcgc accgcttctc gccgcacgac tacgaggcct
     2281 tcgcgaccag cttcggcttc gaggagacgc ccgaccagcg cgccgcgatc catgccgtga
     2341 tccaggacat ggtcagcccg aagccgatgg accgcctcgt ctgcggcgac gtgggcttcg
     2401 gcaagaccga ggtcgcgctg cgcgccgcct tcgtcgcggt gcacggcggc aagcaggtcg
     2461 cgctgctcgc gccgaccacg ctgctggccg agcagcacta ccagaccatc agcgcgcgct
     2521 tcggccagtg gccggtgaag gtggcggaga tgtcgcgctt tcgcaccgcc aaggagatcg
     2581 ccgccgcgat ggcgggcctg gccgacggca cgatcgacat cgtcgtcggc acccacaagc
     2641 tcctgtcgca ggacgtgaag ttcaagcgcc tgggcctgct gatcatcgac gaggagcacc
     2701 gcttcggcgt gcgccacaag gaggcgatga aggcgctgcg ggccgaggtc gacgtgctca
     2761 cgctcaccgc gacgcccatc ccgcgcacgc tgggcatggc gctggaaggc ctgcgcgacc
     2821 tgtccgtgat cgcgaccgcg ccgcagcggc ggctggcgat caagaccttc gtgcgcagcg
     2881 agagccaggg cacgatacgg gaggccgtgt tgcgcgagct caagcgcggc ggccaggtct
     2941 acttcctgca caacgaggtc gagacgatcg ccaaccggcg cgacaagctc gccgagctgc
     3001 tgcccgaggc gcgcatcgcg atcgcccacg gccagatgcc cgagcgcgag ctcgaggcgg
     3061 tgatgcgcga cttcgtggcc cagcgcgtga acgtgctgct gtgctccacc atcatcgaga
     3121 ccggcatcga cgtccccacc gccaacacca tcgtcatcag ccgcgccgac aagttcggcc
     3181 tcgcccagct gcaccagctg cgcggacgcg tcggccgctc gcaccaccag gcctacgcct
     3241 acctgctcgt gcccgacgtc gagggcctga ccaagcaggc cgcgcagcgg ctcgacgcga
     3301 tccaggcgat ggaggagctg ggctcgggct tctacctcgc gatgcacgac ctcgagatcc
     3361 gcggcgccgg cgaggtgctg ggcgagagcc agagcggcaa catgcaggag gtcggcttcc
     3421 agctgtacaa cgacatgctg gccgaggccg tgcgcgcgct gaagtccggc caggagcccg
     3481 acctgctctc gcccaccggc gcgttcggcg gcggcatcga catcaagctg catgccccgg
     3541 cgctgctgcc cgacgcctac tgcggcgacg tgcagacgcg gctgtcgctg tacaagcgcc
     3601 tcgcgaccgc cgaccgcccc gagcagctcg acgcgctgct cgaggagatc accgaccgct
     3661 tcggcaagct gccgccgcag ggccaggcgc tgttcgacac gcaccggctg cgcatcctcg
     3721 cgaagagcta cggggtgctg aaggtcgatg cgaaccccaa ggtcatgaac atcaccttcc
     3781 ggcccaaccc gccgatcgat ccgctgcgca tcatcgagct cgtgcagaag aaccgcgtcg
     3841 tcaagctcgc aggcaacgac cgcctgcgca tcgaccgcga gatcgccgag ccgcgcgacc
     3901 gcgcgcagtt cgtgcgcgac ctgctgcgct cgctggggcc gccgaagacg ccagcctgag
     3961 gcggctgtcg cccgggcgcg cgagcccacg gcgcggtcct tcgtccatcg gcgctttcac
     4021 cgaggcgcac gctcaggtcg ccggcgcgac cgacgtgttc gggatcacga gcgtcgacga
     4081 ctcgaacaac accatcacgg tcggctgcac gcagcaggtc gcgagcgtca gcggcccgca
     4141 cagctacgtt ctcgcgcagc cgcacggtgg aactgcagag ccgcgccggc ggctgacggc
     4201 gcgccttcag gcggtgcgcg aatcgtcccc gagccgctcg gtctcctcga tcggctcgtc
     4261 gtcgtccggg ccgaggcccg gccggtcgcc gacgaggttc tcgatctcct gcgcgacctc
     4321 ggggtcgtcc agcgacgcgg tcgacgcgcc ctccggcgcc gggatgtcgt ccggatcgat
     4381 gacgggcgag ccctcgccga gcggcggcgg cgcgtccggg tcggggatgt ccgagcgatt
     4441 gcccggcagg tcggcgcggt cctggcgcgc catgaggctg tcgacgggct ggacgcggtc
     4501 gatgtcgtcg agcggatcgg cgacgggctt gacgatgggt tgggccatgg ggtctccggt
     4561 ggatctcgac cccctgcggc aagccgggtg cctttcgcgg caattccgcc cttgacagcg
     4621 ctcggcgcgg tgcaggcaga atactggaca tgaatccagt gtcctcgtcg atcgtcgtgc
     4681 cgctgctgtt cctcgccgcc ctcgcggcca tcgccgtgct ggcgctcggc cgccggcgcc
     4741 tgcgcgcgcg gctcgacgag gcgcaggccg ccaccgcggc gacgcaggtc gagctc
//
LOCUS       DASVLN010000926         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_46869, whole genome shotgun sequence.
ACCESSION   DASVLN010000926 DASVLN010000000
VERSION     DASVLN010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46869"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VF453_21695"
     CDS             complement(<1..265)
                     /locus_tag="VF453_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690335.1"
                     /translation="MSDRPTSLDPHSPFVSRLHAAVLTTRIAVLVEADGRVLDVHVAH
                     PSGADAEHLILDGALVEAFRRFHFRPGESAGRPQRGWSTIECAL"
     gene            complement(277..2775)
                     /locus_tag="VF453_21700"
     CDS             complement(277..2775)
                     /locus_tag="VF453_21700"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7690336.1"
                     /translation="MLAITALAASMAAERALAQAAPAAAPGASAPADATGSGRTDRDE
                     KDLTQTQQVVVTGNTRREGMLKKEAGYSITTADAEQIKDAAPTDTADLLKIVPGVFVE
                     TTGGQAGANIRVRGFPEAGDSPYATMQFNGSPIYAAPTLSFVEGSSLFRIDDTIDHVE
                     VLRGGTSPIYGSGQPGVSVNFVQRKGTDAPEGDIRLTAGDKGTGGLRRLDGYYSGKLA
                     DKWYVTIGGFYQSSKGERDTQYPSDRGGQISGNLTHKLDDGGELEIYARSMNTSAAWY
                     TDVPVTFSADGKHVSSYLGVNPHTFTYYDSETRFATIETSPNASGQPGTTTLDFGKGR
                     GPTLTNVGFVLDQKIGDWAFNNKANYTTGDVPSDGLVNGGTPVTLGSYIGGKASANGA
                     TAGSATYVGTGASVDPNQMVMEVGAWQVRKKLQSFTEEAHVSKEIVRNDTLTLGAYFA
                     DYSATDAWDLGNTELMTYENRPRLVDVALNNGDKVSKNGLTSEGGFIDNSSWNGRNTA
                     LYIADEWKPLDGLRLDAGLRREHQSLTGSMAKTSTADLDGDPTTLYDNNGAYVSGGTN
                     TFSETATRTSWTLGANYDFNKQYSMFVRANSGVHLPMFDDVRSNTGGCTNGTSCADVE
                     KIQQFELGFNAVTSWYTAYLSLYTNTLKNSQSQVFSVTGASTTFPSSSRSTGLEFETT
                     IRPVRNFDITWSGDVARAKYFDSGDVASGGLDGLRVARMPGFESRLTPSYRIPTDIGS
                     FRVFGTYSWIAHRYSDAQNTQYLPAYGTLDLGASASLANGMDLRLSVTNVTNTLGLTE
                     GNPRATGSGLNTSNVAMARPLFERAVQVSVGYSF"
BASE COUNT          405 a         1014 c         1131 g          554 t
ORIGIN      
        1 gcaacgcgca ctcgatggtg ctccatccgc gctgcgggcg gccggcggac tcgccggggc
       61 ggaaatggaa gcgccggaac gcctccacca gcgcgccgtc caggatcagg tgctccgcgt
      121 ccgccccgct cggatgcgcg acgtgcacgt cgaggacgcg cccgtcggcc tccaccagca
      181 cggcgatccg cgtcgtcagg accgccgcat gcaggcgact cacgaagggg ctgtgcgggt
      241 cgaggctggt cgggcggtcg ctcatggccg tgaccgtcag aacgaatagc ccaccgagac
      301 ctgcaccgcg cgctcgaaca gcggccgcgc catcgccacg ttgctcgtgt tgagcccgga
      361 gcccgtcgcg cgcgggttgc cttccgtcag ccccagcgtg ttggtcacgt tggtgacgga
      421 caggcgcagg tccatgccgt tggccaggct cgcggaggcg ccgaggtcga gcgtgccgta
      481 ggccggcagg tactgcgtgt tctgcgcgtc gctgtagcga tgtgcgatcc agctgtaggt
      541 gccgaacacg cggaacgagc cgatgtccgt cgggatgcgg tacgacggcg tgaggcgcga
      601 ctcgaagccc ggcatgcgcg cgacgcgcag gccgtcgagg ccgccgctcg cgacgtcgcc
      661 cgagtcgaag tacttggcgc gcgcgacgtc gcccgaccac gtgatgtcga agttgcgcac
      721 cgggcggatc gtggtctcga actcgaggcc ggtcgagcgc gaggacgacg ggaaggtggt
      781 gctcgcgccg gtgaccgaga acacctgcga ctgcgagttc ttcagcgtgt tcgtgtacag
      841 cgacaggtag gccgtgtacc acgacgtcac ggcgttgaag ccgagctcga actgctggat
      901 cttctcgaca tcggcgcagc tcgtgccgtt cgtgcagccg cccgtgttgc tgcggacgtc
      961 gtcgaacatc ggcaggtgca cgccgctgtt ggcgcgcacg aacatgctgt actgcttgtt
     1021 gaagtcgtag ttcgcgccga gcgtccacga ggtgcgggtc gcggtctccg agaacgtgtt
     1081 cgtgccgccg ctgacgtagg cgccgttgtt gtcgtagagc gtggtgggat cgccgtcgag
     1141 gtcggcggtg ctggtcttgg ccatgctgcc ggtcaggctc tggtgctcgc ggcgcaggcc
     1201 ggcgtccagg cgcagcccgt ccagcggctt ccactcgtcg gcgatgtaga gcgcggtgtt
     1261 gcggccgttc cagctcgagt tgtcgatgaa gccgccctcg gaggtgaggc cgttcttcga
     1321 gaccttgtcg ccgttgttca gcgcgacgtc cacgaggcgc gggcggttct cgtaggtcat
     1381 cagctcggtg ttgccgagat cccaggcgtc ggtcgccgag tagtcggcga aataggcgcc
     1441 gagcgtcagc gtgtcgttcc tgacgatctc cttgctgaca tgcgcctcct cggtgaagga
     1501 ctgcagcttc ttgcgcacct gccaggcgcc gacctccatc accatctggt tcgggtcgac
     1561 ggacgcgccg gtgccgacgt aggtagcgga gccggccgtg gcgccgttgg ccgaggcctt
     1621 gccgccgatg tagctgccca gggtcaccgg cgtgccgccg ttgacgaggc cgtcggacgg
     1681 cacgtcgccg gtggtgtagt tggccttgtt gttgaaggcc cagtcgccga ttttctggtc
     1741 gaggacgaag ccgacgttgg tgagcgtcgg gccgcggccc ttgccgaagt ccagcgtcgt
     1801 cgtgccgggc tggccgctcg cgttgggaga ggtctcgatc gtcgcgaagc gggtctcgct
     1861 gtcgtagtac gtgaaggtgt gcgggttgac gccgagatag gacgacacgt gcttgccgtc
     1921 ggcgctgaag gtgaccggca cgtcggtgta ccaggccgcg ctcgtgttca tcgagcgcgc
     1981 gtagatctcg agctcgccgc cgtcgtcgag cttgtgcgtc aggttgccgc tgatctggcc
     2041 gccgcggtcg gacgggtact gcgtgtcgcg ctcgcccttg ctgctctggt agaagccgcc
     2101 gatcgtcacg taccacttgt cggccagctt gccggagtag tagccgtcga gtcgccgcag
     2161 cccgcccgtg cccttgtcgc cggcggtcag gcggatgtcg ccctcggggg cgtccgtgcc
     2221 cttgcgctgg acgaagttga ccgagacgcc cggctgcccg gagccgtaga tcgggctcgt
     2281 gccgccgcgc agcacctcga cgtggtcgat ggtgtcgtcg atgcggaaca gcgacgagcc
     2341 ctcgacgaag gacagcgtcg gcgccgcata gatcggcgag ccgttgaact gcatggtcgc
     2401 gtacggcgag tcgcccgctt cggggaagcc ccgcacccgg atgttggcgc ccgcctggcc
     2461 gcccgtggtc tcgacgaaga cgcccggcac gatcttgagc aggtcggcgg tgtcggtcgg
     2521 cgcggcgtcc ttgatctgct cggcgtccgc cgtcgtgatc gagtagccgg cctccttctt
     2581 gagcatgccc tcgcggcgcg tgttgccggt gacgacgacc tgctgggtct gggtcaggtc
     2641 cttctcgtcc cggtccgtgc ggccggagcc cgtggcgtcg gccggtgccg aggcgcccgg
     2701 cgcggcggcc ggcgccgcct gtgcgagggc gcgctcggcg gccatcgagg ccgcgagcgc
     2761 ggtgattgcg agcatcaggg tgctcggagt cgatcttgtt cgggtcattg cttgtcgccc
     2821 caccgtgggt tgtccatccg ggtcgccgcg cgcctcttgc cgcgcggccg ccccggctcc
     2881 gtggcggtgc gccgggtcgc ccgagggcgc ctggcgtcta ccgggcgccg ggcttgtctc
     2941 cggcccggcg cctgaagtca atgtaggaag ccaaatcgac ggcaggattt tgttcttgaa
     3001 ccccgcacgc aaacttggtt tcgaaagccg cggccgcctg caacgaatga aacatgcgac
     3061 ccacgggagt ccacgttcgc cgccatcatg ggcgcgatca cgcc
//
LOCUS       DASVLN010000927         2873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_103505, whole genome shotgun sequence.
ACCESSION   DASVLN010000927 DASVLN010000000
VERSION     DASVLN010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103505"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..813)
                     /locus_tag="VF453_21705"
     CDS             complement(304..813)
                     /locus_tag="VF453_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7690337.1"
                     /translation="MNKTQPESSSAASMSMDKGIDKIDARILRVLQRDGRISNLKLAE
                     EVHLSPTAVLERVKRLTRENYILGYEARLNPNKLGASMMVFIEVLLDRTAPDIMDTFK
                     AAVQTRPEILECHLVAGGFDYLIKTRVADMAAYREMIASVIWSLPGVRETRTYTVMEE
                     VKTTNALPI"
     gene            910..>2873
                     /locus_tag="VF453_21710"
     CDS             910..>2873
                     /locus_tag="VF453_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX7690338.1"
                     /translation="MPTSSPDFVARPQQARLPYPYRREEDVVGAALATLLGSLDWSAV
                     VRDARPWIEAVRDRPAPFWAMESLLREYPISSAEGLALMRLAEALLRVPDVETAIALT
                     ADQLGRAEFDGEAAPDGPHRMLANLSASAISLSKKFLPGAEGEGGLLKRLGAKTVVAA
                     TVRAIQLLGRQFVLGRTIAEAMGEAQEQRKAVDAARRGTPAAGNALRFSYDMLGEGAR
                     TEHDAERYLASYANAISAISRGQVAASPQTADGISIKLSALFSRYEDAQRQRVFDELL
                     PRVWQLIQRAAAANLNLTIDAEESDRLELSLDVLDALAERVAGTFPKWGGLGLAVQAY
                     QTRALEVVDAVAEIARRHGLRFMVRLVKGAYWDGEIKRAQEGGLAGYPVFTHKSHTDV
                     SYLACAQRLIAHADVIYPQFATHNAGTISAILQMARAKGAAFEMQRLHGMGEGVYREV
                     MKDPAIACRVYAPVGEHRDLLAYLVRRLLENGANSSFVHQLADATVDVEQLLASPLVA
                     THAPAFPMPADLYPTQHGLRRRNSTGADLAVIADRAPLEAAVASTTVPVVAEATPAEC
                     AATMARLAEGFRAWDERPVSERAAIVRRAGDLLDARLAEFCGLLVKEAHKTLGDCVAE
                     VREAVDFCRYYANQAEAGLGDEPLPGPTGE"
BASE COUNT          409 a         1072 c          970 g          422 t
ORIGIN      
        1 gggccgggcc cggcccgtcg gcgacggccg gctcggaggc ctgcgccggg acggcgcgcg
       61 ggtcggcggg gtcggcagga aacatcggca acggtcttct cgcgcacccc gggcgacggc
      121 accgcgccgt cgcatcgacc ggggatctcg ctacggtgcc ggatcacccc cacccgcgtt
      181 cactgaattg ggcgccccat cccgacgaat cttcggcatc cggcgagttc gacccccatc
      241 acatcggagc ccgaaagcgc gagacacggc gccgcgggag cgccagcgac cgtggaggct
      301 ccgtcagatc ggcagggcgt tggtcgtctt gacctcttcc atcaccgtgt aggtgcgcgt
      361 ctcgcgcacg cccggcaggc tccagatgac cgaggcgatc atctcgcgat aggcggccat
      421 gtccgccacg cgggtcttga tcaggtagtc gaagccgccc gcgacgaggt ggcattcgag
      481 gatctccggc cgcgtctgca ccgcggcctt gaaggtgtcc atgatgtccg gcgcggtgcg
      541 atcgagcagc acctcgatga acaccatcat gctcgcgccg agcttgttgg gattcaggcg
      601 cgcctcgtag ccgaggatgt agttctcgcg cgtgagtcgc ttcacgcgct cgagcaccgc
      661 cgtcggcgac aggtgcacct cctcggcgag cttcaggttg ctgatgcgtc cgtcccgttg
      721 caggacgcgg aggatgcgtg cgtcgatctt gtcgatgccc ttgtccatcg acatcgaggc
      781 ggccgatgac gactccggct gggtcttgtt catgctggac gaatctccgc gtgctctgac
      841 gaaatccgaa tttatctctt ggattctcgc cctaagctat caaattatcc agtcaggatt
      901 tctaccgcca tgccaacatc cagccccgac tttgtcgcgc gcccgcaaca ggcacgactg
      961 ccctatccct atcgccggga ggaggacgtg gtcggcgcgg cgctggcgac gctgctgggc
     1021 tcgctggact ggtcggccgt cgtccgcgac gcgcgcccgt ggatcgaggc cgtgcgcgac
     1081 cggccggcgc cattctgggc gatggagtcg ctgctgcgcg agtatccgat ctccagcgcc
     1141 gagggcctcg cgctgatgcg cctggccgag gcgctgctgc gcgtgcccga cgtcgagacc
     1201 gcgatcgcgc tcaccgccga ccagctcggg cgcgccgagt tcgacggcga ggccgcgccc
     1261 gacggcccgc accgcatgct ggccaacctg tcggcgagcg cgatctcgct gtccaagaag
     1321 ttcctgcccg gcgccgaggg cgagggcggc ctgctcaagc gcctgggcgc gaagacggtg
     1381 gtcgccgcca ccgtgcgcgc gatccagctg ctcgggcgcc agttcgtgct cgggcgcacg
     1441 atcgccgagg cgatgggcga ggcccaggag cagcgcaagg ccgtcgacgc cgcgcgccgc
     1501 ggcacgccgg ccgccggcaa cgcgctgcgc ttctcctacg acatgctggg cgaaggcgcc
     1561 cgcaccgagc acgatgccga gcgctacctc gcgtcgtatg ccaacgcgat ctcggcgatc
     1621 tcgcgcggcc aggtcgccgc ctcgccgcag acggccgacg gcatctcgat caagctgtcg
     1681 gcgctgttct cgcgctacga ggacgcccag cgccagcgcg tgttcgacga gctgctgccg
     1741 cgcgtgtggc agctgatcca gcgcgccgcc gccgccaacc tgaacctcac catcgacgcc
     1801 gaggagagcg accggctcga gctgtcgctg gacgtcctcg acgcgctcgc cgagcgcgtg
     1861 gccggcacct tcccgaagtg gggcggcctc ggcctggccg tgcaggcgta ccagacgcgc
     1921 gcgctcgagg tcgtcgacgc cgtcgcagag atcgcccgcc gccacggcct gcgcttcatg
     1981 gtgcgcctgg tcaagggcgc ctactgggac ggcgagatca agcgcgcgca ggagggtggc
     2041 ctcgccggct acccggtgtt cacgcacaag tcgcacaccg acgtctccta cctcgcctgc
     2101 gcccagcgcc tgatcgccca cgccgacgtg atctacccgc agttcgcgac ccacaacgcc
     2161 ggcacgatct ccgcgatcct gcagatggcg cgcgcgaagg gcgccgcctt cgagatgcag
     2221 cgcctgcacg gcatgggcga gggcgtgtac cgcgaggtca tgaaggatcc ggcgatcgcc
     2281 tgccgcgtct acgcgcccgt cggcgaacac cgcgacctgc tcgcgtatct cgtgcgccgc
     2341 ctgctggaga acggcgccaa ctccagcttc gtccatcaac tggccgacgc gacggtcgac
     2401 gtcgagcagc tgctcgcctc gccgctcgtc gccacccacg cccccgcctt cccgatgccc
     2461 gccgatctct acccgaccca gcacggcctg cgccgccgca actccaccgg cgccgacctc
     2521 gccgtcatcg ccgaccgcgc cccgctcgag gcggccgtcg cctcgacgac cgtgcccgtc
     2581 gtcgccgagg ccacgccggc cgaatgcgcc gccacgatgg cgcgcctggc cgagggcttc
     2641 cgcgcctggg acgagcgccc ggtctccgaa cgcgccgcga tcgtccgccg cgccggcgac
     2701 ctgctcgacg cccgcctggc agagttctgc ggcctgctcg tgaaggaagc gcacaagacg
     2761 ctcggcgact gcgtggccga agtgcgcgag gcggtcgact tctgccgcta ctacgcgaac
     2821 caggccgagg ccggcctggg cgacgagccg ctgccgggcc cgaccggcga gag
//
LOCUS       DASVLN010000928         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_371520, whole genome shotgun sequence.
ACCESSION   DASVLN010000928 DASVLN010000000
VERSION     DASVLN010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371520"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2252
                     /locus_tag="VF453_21715"
     CDS             <1..2252
                     /locus_tag="VF453_21715"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HEX7690339.1"
                     /translation="IAPPAPAAESLLLAVRVNGIDGDDGVRVVRIAGRVALQRADWNT
                     LHLRAAAGSPVRVDGQDYLPLDGLGGLRWRIDEASQTLVIDAPATAFAGTHLRMDVEA
                     TAPVVPAATGAYGNYDVELQRTVEGGDPHSRQSASAQLEAGVSALGGSLATSALLLDD
                     GVNRRATRLDATWTQESPRHLASLRAGDAISSPGMWGRALRFGGLQWSTDFSLKPGFL
                     PFPLPSMHGEAALPSTVDIYVNNARRLQGDVPAGPFDVSDLPIVTGSGDVRLVVRDAL
                     GREQVVTQPYFVSPSLLKPGLHAFSVEAGAVREDYALRSDHYGRLMASATDRAGVSDS
                     FTRELRVEFGPGLAAAGATGIWLLPRAGIATLGAMASHGAEGDGGAVTASLSRQGHAW
                     NASVQAQRNLRAFSQLGVPARDALRSQATATVGGSVAGLPVGAGFVYQAPWNAAPQRL
                     LSVNAGAGLGRLGTLGFYGLRDLGTRATTLSLSYSVSLDARTGIATGATRSGGPGLAT
                     ARTGQVDIERNLPDGDGLGWRIDAQDDDRAQARLFARTDRADLEAGIARANRQTDVRV
                     GAAGGVAWIDGTAFVSRRVDGAYAVVEVGDYPGITITRDNHPVARTDEHGRALVAGLR
                     GNEVNHIGVEAAELPMDAAVDALDLDFIPPAHAGVVLRLPIERSRSATLRVLGRDGMP
                     LPPGTVLRVAGRDASFPVGFDGRTFVSGLRAGTRLRARGPGADCGFALAVPEGDADDL
                     PDVGNVVCQ"
     gene            2261..2785
                     /locus_tag="VF453_21720"
     CDS             2261..2785
                     /locus_tag="VF453_21720"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HEX7690340.1"
                     /translation="MRPLMPLAASLRTLAALAAVLSALLGAAPAQALCVGVCSCGVTT
                     ANIVFGNINPLNPSNADATGSVTVSCSGVAGLLIPVTVDLSKGGGASFASRSMSGGSG
                     RLQYNLYADSAHTQVFGDGTASTVDGNASILLNALGQGPAANFNVYGRVFGSQTAAVP
                     GAYSDTLAVTLTYY"
BASE COUNT          374 a         1115 c         1047 g          402 t
ORIGIN      
        1 cgatcgcgcc gcccgcgccg gcggccgagt ccctgctgct ggcggtccgc gtgaacggca
       61 tcgatggcga cgacggcgtg cgcgtcgtcc gcatcgcggg acgcgtcgcg ctgcagcgcg
      121 ctgactggaa cacgctgcac ctgcgcgcgg ccgcaggatc gccggtgcgg gtcgacggcc
      181 aggactacct gccgctcgat ggcctgggtg gcctgcgctg gcggatcgat gaggccagcc
      241 agaccctcgt gatcgacgcg ccggcgacgg cctttgccgg cacgcacctg cgcatggacg
      301 tcgaggcgac cgcccccgtc gtcccggccg ccacgggtgc gtacggcaac tacgacgtcg
      361 aattgcagcg cacggtcgag ggtggcgacc cccactcgcg ccagtcggcc tcggcgcagc
      421 tcgaggcggg cgtctcggcg ttgggcggat cgctcgcgac gagcgcactg ttgctggacg
      481 acggcgtcaa ccgccgcgct acgcgcctgg acgcgacctg gacgcaggag agcccgcgcc
      541 acctggcgag cctgcgcgcg ggcgacgcga tcagctcgcc gggcatgtgg gggcgtgcgt
      601 tgcgcttcgg cggccttcag tggtcgaccg acttctcgct caagccgggc ttcctgccgt
      661 tcccgctgcc gtccatgcac ggcgaggccg cgctgccgtc gaccgtcgac atctacgtca
      721 acaacgcacg ccggctgcag ggcgacgtgc ccgccgggcc gttcgacgtg agcgatctgc
      781 ccatcgtcac gggaagcggc gacgtccggc tggtcgtgcg cgacgcgctg gggcgcgagc
      841 aggtggtcac gcagccctac ttcgtcagcc cgtcgctgct caagcccggg ctgcacgcgt
      901 tctcggtcga ggccggcgcc gtgcgcgagg actacgcgct gcgcagcgat cactacggcc
      961 gcctgatggc ctcggccacc gaccgcgccg gcgtctccga ctcgttcacg cgcgagttgc
     1021 gcgtcgagtt cgggcccggt ctcgccgcgg ccggcgcgac gggcatctgg ctgctgccgc
     1081 gagccggcat cgcgacgctc ggggcgatgg ccagccacgg tgcggagggc gacggtggcg
     1141 cggtcacggc gtcgttgagc cgccagggcc acgcctggaa cgccagcgtg caggcccagc
     1201 gcaacctgcg cgccttctcg cagctgggcg tgccggcacg cgatgccctg cgttcgcagg
     1261 cgaccgcgac ggtcggcggc agcgtcgcgg gcctgcccgt cggcgcgggc ttcgtctacc
     1321 aggcgccctg gaacgcggcg ccgcaacgac tgttgtccgt caacgccggc gccggactcg
     1381 ggcgcctcgg cactctcggg ttctacggcc tgcgcgacct gggcacgcgc gccacgacgc
     1441 tctcgctgag ctacagcgtc tcgctcgacg cgcgcaccgg catcgcgacg ggtgccacgc
     1501 gcagcggcgg accggggctc gcaacggcgc gcacgggtca ggtggacatc gagcgcaacc
     1561 tgccggacgg cgatggcctg ggctggcgca tcgacgcgca ggacgacgat cgcgcgcaag
     1621 cacggctgtt cgcgcgcacc gaccgcgccg acctcgaggc gggcatcgcg cgcgccaacc
     1681 ggcagaccga tgtacgtgtc ggcgccgcgg gcggggtggc atggatcgac ggtacggcgt
     1741 tcgtctctcg gcgcgtcgac ggcgcctacg ccgtcgtgga ggtcggcgac tatcccggga
     1801 tcactatcac gcgcgacaac catccggtcg cgcgcaccga cgagcacggc cgagcgcttg
     1861 tcgccggcct gcgtggcaac gaggtcaacc acatcggcgt cgaggccgcc gaactgccga
     1921 tggacgccgc cgtcgatgcg ctcgacctgg acttcatccc gcccgcgcat gccggagtgg
     1981 ttctgcgcct gccgatcgag cgcagccgct ccgcgacgct gcgtgtcctc gggcgggacg
     2041 gcatgccgct tcctcccggg accgtgctgc gcgtggccgg gcgagacgcg agcttccccg
     2101 tcggcttcga cggccgcacc ttcgtctcgg gcctgcgcgc cggcactcgc ctgcgcgcac
     2161 gcggcccggg cgccgactgc ggattcgcgc tcgccgtgcc cgaaggcgac gccgacgacc
     2221 tgccagacgt cggcaacgtg gtctgccaat gaacggtccc atgcgccccc tcatgcctct
     2281 ggcggcatcc ctgcgcacgc tcgcggcgct tgccgcggtg ctgtccgcgc tgctgggcgc
     2341 cgcgcccgct caagcactgt gcgtcggcgt ctgcagctgc ggcgtcacga ccgccaacat
     2401 cgtcttcggc aacatcaacc cgctgaatcc gtccaatgcc gacgccaccg gcagcgtgac
     2461 cgtgagctgc agcggcgtcg cgggattgct gattccggtg accgtcgacc tcagcaaggg
     2521 cggcggcgcc agcttcgcca gccgcagcat gagcggcggc agcggccgcc tgcagtacaa
     2581 cctctatgcc gacagcgcgc acacgcaggt cttcggcgac ggcacggcct cgaccgtcga
     2641 tggcaacgcc agcatcctgc tgaacgcgct cggccagggc cccgctgcga acttcaacgt
     2701 ctacggccgg gtattcgggt cgcagaccgc cgccgtgccg ggcgcctact cggacacgct
     2761 cgcggtgacc ctgacctact actgaatcgg cggcaggctc gcggcgtgca tttgtgcacg
     2821 ccacgcggac acggcaggcc cgcctccccg ccgcgagcgc cgccggcgcc gcggagcccc
     2881 gggcgacgct ccataatgcg gcgcatgccg ctcgcccgcc ctgcccgcgc cctcgccg
//
LOCUS       DASVLN010000929         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_209942, whole genome shotgun sequence.
ACCESSION   DASVLN010000929 DASVLN010000000
VERSION     DASVLN010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209942"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1142
                     /gene="malE"
                     /locus_tag="VF453_21725"
     CDS             3..1142
                     /gene="malE"
                     /locus_tag="VF453_21725"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HEX7690341.1"
                     /translation="MCACPAPAAAPLHLLVWINPDKGYNGLQKVGDRYAADTGVEVVV
                     QHPDDAPSKFQAAAGAGKGPDIFCWPHDRAGEWAKSGLIVPVKPRDSLRATIEESAWK
                     AFTYQGKVWGYPLAIEAIGLIYNKALVPVPPTSIDEVVALDRKLSAQGKKAILWDYNK
                     TFFTWPLLAGAGGYIYGRTPDGGYDTDDVGVNAPGAVRAATVLQGLVRSGVLPKGAGY
                     SEMEGGFNRGEVAMMISGPWAWDNVKKSHIDFGVAPLPAVDGHPAKPFVGVLGCMVAA
                     PSRNKDLARDFLENYVLSLDGLKTINADVPLGTPANKRFFDELKGDPHIAATMDNARR
                     GEPVPNIPEVGKFWTAMDAALEAITNMRQSPKDALDGAKARILVK"
     gene            1151..>1610
                     /gene="malF"
                     /locus_tag="VF453_21730"
     CDS             1151..>1610
                     /gene="malF"
                     /locus_tag="VF453_21730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856690.1"
                     /note="with MalKGE is involved in maltose transport into
                     the cell; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HEX7690342.1"
                     /translation="MTSTSAPAPSAGRRLLAPLALALVAAAGLWLVFTVYASGQTLLA
                     LAALVLLAAGLYVYGARGVLAWRYLFPGIVGMGLFVAFPLLYTMQIGFTNYSSAHLLS
                     QERVKAYLLEQTVPDEEHALEFALLPDPQDASRQRIVIGPHDDAAPFASAA"
BASE COUNT          243 a          597 c          538 g          232 t
ORIGIN      
        1 cgctgtgcgc ctgccccgcg cccgcggccg cgccgctgca cctgctggtc tggatcaatc
       61 ccgacaaggg ctacaacggc ctgcagaagg tcggtgaccg ctacgccgcc gacaccggcg
      121 tcgaggtggt cgtccagcat cccgacgacg cgccgtccaa gttccaggcc gctgccggcg
      181 ccggcaaggg ccccgacatc ttctgctggc cgcacgaccg ggcgggcgag tgggcgaagt
      241 cgggcctgat cgtgccggtc aagccgcgcg actcgctgcg cgcgaccatc gaggagtccg
      301 cctggaaggc cttcacctac cagggcaagg tctggggcta cccgctcgcg atcgaggcga
      361 tcggcctgat ctacaacaag gccctggtcc cggtgccgcc gacatcgatc gacgaggtcg
      421 tcgcgctgga caggaagctg tcggcgcagg gcaagaaggc gatcctctgg gactacaaca
      481 agaccttctt cacctggccg ctgctggccg gtgcgggcgg ctacatctac ggccgcacgc
      541 ccgacggcgg ctacgacacc gacgacgtcg gcgtgaacgc gcccggcgcg gtcagggcgg
      601 cgaccgtgct gcaaggcctc gtcaggagcg gcgtgctgcc caagggcgcg ggctactccg
      661 agatggaggg cggcttcaac cgcggcgagg tcgcgatgat gatctcgggc ccctgggcct
      721 gggacaacgt gaagaagagc cacatcgact tcggcgtcgc gccgctgccc gcggtggacg
      781 gccatcccgc caagcccttc gtgggcgtgc tgggctgcat ggtcgccgcg cccagccgca
      841 acaaggacct cgcgcgcgat ttcctcgaga actacgtgct ctccctcgac ggcctgaaga
      901 cgatcaacgc cgacgtcccg ctcggcacgc cggccaacaa gcgcttcttc gacgagctca
      961 agggggaccc gcacatcgcc gcgacgatgg acaacgcgcg ccgcggcgag cccgtgccca
     1021 acatccccga ggtcggcaag ttctggaccg cgatggatgc cgccctcgag gccatcacca
     1081 acatgcgcca gagcccgaag gacgcgctgg acggcgccaa ggcccggatc ctcgtcaaat
     1141 gagcgccgtc atgacctcca cttccgcgcc cgcgccttcc gcgggccgca ggctcctggc
     1201 gccgctcgcc ctcgcgctcg tcgcggccgc cggcctgtgg ctcgtgttca ccgtgtacgc
     1261 gtcggggcag acgctgctcg cgctcgcggc gctggtgctg ctcgccgcag gcctgtacgt
     1321 ctacggcgcg cgcggcgtgc tcgcgtggcg ctacctgttc ccgggcatcg tcggcatggg
     1381 cctgttcgtc gcgttcccgc tgctctacac gatgcagatc ggcttcacga actactcgtc
     1441 ggcccacctg ctgagccagg agcgcgtgaa ggcctacctg ctggagcaga ccgtgcccga
     1501 cgaggaacac gccctggagt tcgcgctgct gcccgacccg caggacgctt cacgccagcg
     1561 catcgtgatc ggcccccacg acgatgccgc gcccttcgcg agcgccgcct
//
LOCUS       DASVLN010000930         1665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_479925, whole genome shotgun sequence.
ACCESSION   DASVLN010000930 DASVLN010000000
VERSION     DASVLN010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479925"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1665)
                     /locus_tag="VF453_21735"
     CDS             complement(<1..>1665)
                     /locus_tag="VF453_21735"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX7690343.1"
                     /translation="RTLQAPGRRPAILLATEDAAHPCVARIDDGFLWSPLRQATDDAA
                     WTVRDETGRLLFQAAGGDARGARSTRPQHTEGHLFLGVDFGAGQWVLDQATPAPEVRW
                     HGMRVSTWLVSVAVLTVLVVGLIGQSRLRVALAPLEQLTDGVRRLAHGAPLARVPVRR
                     ADEIGTLTAAFNDMSTQLAGRLASLRGLAAIDAAILGRRPFADIGAMALRRLAEIHPR
                     HDVLVAWRRDGAGLAWLHREADAARETAGGGDLPVHGLGADPAFAGAVEAVAPCALDA
                     LRDEEAGTRLPDAFVAPAAAGWQALAVREGGSVRAMIALRAAAGAAPAELHEARALRD
                     RLAVAIVARSREDELVHRAAHDTLTGLLNGYGLLQALSARLADGAPLALAFIDLDEFK
                     IVNDRFGHAEGDRLLESVARRLRTLVPEGVPLARYGGDEFVAILPGADAEAAQAVAAA
                     VVAGLQAPFSLAAVEHLCGASVGVALHPAHAADAPELLRCADIALYEAKRRGRGRHVM
                     FDAGQDATLRERAELVADLRAALARSDELVLHYQPRRPAAGGDFCA"
BASE COUNT          196 a          633 c          667 g          169 t
ORIGIN      
        1 cggcgcagaa gtcgccgccc gcggcgggcc gccgcggctg gtagtgcagc accagctcgt
       61 cgctgcgcgc gagggcggcg cgcaggtccg ccaccagctc ggcgcgctcg cgcagcgtgg
      121 cgtcctggcc ggcgtcgaac atcacgtggc ggccgcggcc gcggcgcttg gcctcgtaga
      181 gcgcgatgtc ggcgcagcgc agcagctcgg gcgcgtcggc ggcatgcgcc ggatgcagcg
      241 ccacgccgac gctcgcgccg cacagatgct cgacggccgc gaggctgaac ggcgcctgca
      301 ggccggcgac caccgcggcc gccacggcct gcgccgcctc ggcgtcggcg ccgggcagga
      361 tggcgacgaa ctcgtcgccg ccgtagcgcg ccagcggcac gccctccggc acgagcgtgc
      421 gcaggcggcg cgccacgctt tccagcaggc ggtcgccctc ggcgtggccg aagcggtcgt
      481 tgacgatctt gaactcgtcc aggtcgatga aggccagcgc cagcggcgcg ccgtcggcga
      541 gccgcgccga cagcgcctgc agcagcccgt agccgttcag caggccggtg agcgtgtcgt
      601 gggcggcgcg gtgcaccagc tcgtcctcgc ggctgcgcgc gacgatggcg acggccaggc
      661 ggtcgcgcag ggcgcgcgcc tcgtgcagct cggcgggggc ggcgccggcg gccgcgcgca
      721 gcgcgatcat cgcgcgcacg ctgccgccct cgcgcaccgc gagcgcctgc cagcccgcgg
      781 ccgcaggcgc gacgaacgcg tcgggcaggc gggtgccggc ttcctcgtcg cgcagcgcat
      841 cgagcgcgca gggcgcgacc gcctcgacgg cgcctgcgaa cgcggggtcg gcgccgagcc
      901 catgcaccgg caggtcgccg ccgcccgccg tctcccgcgc ggcgtcggcc tcgcggtgca
      961 gccaggcgag gccggcgccg tcgcgccgcc aggccacgag cacgtcgtgg cggggatgga
     1021 tctcggccag gcgccgcagc gccatcgcgc cgatgtccgc gaagggtcgc cggccgagga
     1081 tggccgcgtc gatcgcggca agcccgcgca gcgaggcgag gcggccggcg agctgcgtcg
     1141 acatgtcgtt gaaggcggcg gtgagcgtgc cgatctcgtc ggcgcgccgc accggcaccc
     1201 gggccagcgg ggcgccgtgc gcgagccggc gcacgccgtc ggtgagctgc tcgagcgggg
     1261 cgagggccac acgcaggcgc gactggccga tcagtccgac cacgagcacg gtcagcaccg
     1321 ccaccgacac cagccaggtg ctgacgcgca tgccgtgcca gcgcacctcg ggcgccgggg
     1381 tggcctggtc gagcacccat tggccggcgc cgaagtcgac gcccaggaac aggtgcccct
     1441 cggtgtgctg cgggcgcgtc gagcgggcgc cgcgcgcgtc gccgccggcg gcctggaaca
     1501 ggaggcggcc ggtctcgtcg cgcaccgtcc acgccgcgtc gtcggtcgcc tggcgcagcg
     1561 ggctccacag gaagccgtcg tcgatgcggg cgacgcaagg atgggcggcg tcctccgtcg
     1621 ccagcaggat ggcgggtcgg cggcccgggg cctgcagcgt gcgca
//
LOCUS       DASVLN010000931        33195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_47151, whole genome shotgun sequence.
ACCESSION   DASVLN010000931 DASVLN010000000
VERSION     DASVLN010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 33195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33195
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47151"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VF453_21740"
     CDS             <1..200
                     /locus_tag="VF453_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690344.1"
                     /translation="WALRLALVGQVAFVAALAAGLRLGGLAGAGWGVSAAMTLYFGWY
                     FLKLATWPLAPESTSNEDTPT"
     gene            197..817
                     /locus_tag="VF453_21745"
     CDS             197..817
                     /locus_tag="VF453_21745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7690345.1"
                     /translation="MTLRATWNRWRRALLRRWAWQPVFGERSHRGAFLKHTRISGATV
                     IEHEDRLALGDNVFIGHFNFIEASGGITIGEGVQVTSHCAIVTHASHRSQRLLGPAYV
                     TWPLTPGTQRPGWIAGPVHIGAYSFIGPHSLIEANTRIGRGSLVCAGSFVRGEYPDFA
                     ILEGRPARVVGDTRRADERLLERYPELRVLYDAWAQAPEPSPFGEP"
     gene            858..1958
                     /locus_tag="VF453_21750"
     CDS             858..1958
                     /locus_tag="VF453_21750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7690346.1"
                     /translation="MTPESSRPLRLVLWGDGESPHLVKWARALASRVELFVASSRGLA
                     PGIEALVAPERRLALGQATKFAGGNAALLKTLPRFAAWVRRVDPDWINAHYLSSHGTM
                     AWLARKLGVRARLAASAWGSDILVAPERSRAMRWVTGRVLRDAAISTSDSAHMAQRMR
                     ALGAREVMTFPFGLDALPPVDPATPKDPRLFFANRGLEDIYAPLRVLSLFASAAAAWP
                     DARLVVANDGSMREAMLRRARQLGIADRVEFTGRLDAAAQARFYARARWYLSVPRSDS
                     VSVSVLEAMAHGCLPILSDLPANRELVRAGHNGVIVEPERGDLELESLLVVDARADAI
                     AALNRDWVGAHGLFGPSVDAFVARLAQLSGRA"
     gene            2022..3305
                     /locus_tag="VF453_21755"
     CDS             2022..3305
                     /locus_tag="VF453_21755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7690347.1"
                     /translation="MNIVYIEHYAGTPALGMEFRPYYLAREWVRAGHRVRMLAASYSH
                     VRARQPAVGTTALDERIDGIDYRWYPTPAYQGNGLGRVKNIFSFLRQVWADTGRLVRE
                     ARPDVIIASSTYPMDIWVARRLARRAGAKLVFEVHDLWPLSPIELSGMSPRHPFARLC
                     SWAEADAYRDSDVVVSMLPCVHEYMASRGLDLRKLAIVPNGISPDDWAAAPAPLREDV
                     AAALDRAREAGETVVGYAGSMGEPNALDTLLDAAALLRDDAAAAGRRIRIVMVGDGHL
                     RAHLAARVQAEGLANVTMLPPIPKPQVPALLAQVDIAYIGWQRVPIYRFGIAPNKLMD
                     YMMAGCAVLHSVEAGNDPVAEAGCGITVAPGDARAVADGLLRLAALPAGERAAMGARG
                     RAFVEAKHAYPVLAARFIEAVEDCASIPAATSGRA"
     gene            complement(3353..4579)
                     /locus_tag="VF453_21760"
     CDS             complement(3353..4579)
                     /locus_tag="VF453_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7690348.1"
                     /translation="MKVLVVSQYFWPETFRVNEIVSELTSRGHEVTVLTGRPNYPEGS
                     VSPDFAAAPQRFAAYAGASVLRVPLRPRGKGNLSLLLNYWSFVFWGCALGPWLLRGRP
                     FDAIFVFETSPITSALPAILLKRIKRAPLSMWVLDLWPDTLKAVGMVKSEFSLRVAGA
                     LCRFIYRRCDLILGQSRAFVEPIRRWEGSSRRFRYFPAWSEEIFDAAAQADALAAELA
                     PHAGSFKIVFAGNIGDAQDFPAVLEAARLTRHRPDVHWLIVGDGRAAPRVRADIDRLG
                     LAATVFMLGRHPVERMPSFFAGADALLVSLKADPIFAMTIPGKVQSYLASGKPLLGML
                     DGEGARVIEEARAGFVAPSGNAAELARLAIALADLSAGERAQMGAQGQRYGEENFKRA
                     TLMSRLEAWLANPASD"
     gene            complement(4587..5720)
                     /gene="wecB"
                     /locus_tag="VF453_21765"
     CDS             complement(4587..5720)
                     /gene="wecB"
                     /locus_tag="VF453_21765"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX7690349.1"
                     /translation="MKKLKVVSVVGTRPEIIRLSRVLARLDEFCDHVLVHTGQNYDYE
                     LNQVFFDDLGVRKPDHFLGSAGSNAAHTIGHLIIAVDDALAKERPDALLVLGDTNSCL
                     AVIPAKRRRIPIFHMEAGNRCFDLRVPEETNRRIVDHTADINLTYSDIAREYLLREGL
                     PPDMVIKTGSPMLEVLSHYRDRIDASDALSRLGLADGRYFVVSAHREENIESDRSFGK
                     LCAVLDGLAEDHGLPVIVSTHPRTQKRITAMGVRFHANVRLLKPLGFHDYVKLQMHAR
                     AVLSDSGTITEESSILNFPALNLREAHERPEGMEEAAVMMVGLELQRIRQALAILERQ
                     PRGGERALRRIADYGMPNVSDKVVRIIHSYTDYVKRVVWRDYE"
     gene            complement(5717..6832)
                     /locus_tag="VF453_21770"
     CDS             complement(5717..6832)
                     /locus_tag="VF453_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7690350.1"
                     /translation="MRIAITGGHGFIGRNLQVRLQESGHGDPLLVPHDAEVPALSALL
                     ADVDVVFHLAGVNRPQDPTEFATGNHDFTRRLCEAMAAQPRPPRVVFASSTQATQDNP
                     YGRSKRQAEDELFSYGRHTGAGVHIYRLTNVFGKWSRPNYNSAVATFCHNIARGEPIV
                     VNKADAPLRLVYVDDVVRHFCALLDAADTPSGLLSAGPEYDTTVGELADQIRAFAASR
                     ESLVTERVGTGLARALYATYVSFLPLPAFEYEVARHADPRGVFVEMLKTPDCGQFGYF
                     TAFPGITRGEHYHHTKTEKFLVIKGTASFGYRHIVTNETHEIVARGGEGRIVETIPGW
                     THNVTNIGDDELVVMVWANEIFDRQRPDTVARKVRQA"
     gene            complement(6834..7868)
                     /locus_tag="VF453_21775"
     CDS             complement(6834..7868)
                     /locus_tag="VF453_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX7690351.1"
                     /translation="MFHDKTLLITGGTGSFGNAVLRRFLHSDLREIRIFSRDEKKQDD
                     MRKRYDDPKLKFYIGDVRDARTVETAMRGVDYCFHAAALKQVPSCEFHPMEAVRTNVL
                     GTENVLEAAIRAGVERVVCLSTDKAVYPINAMGISKAMMEKVMVAASRNLEGSRTVIC
                     GTRYGNVMASRGSVIPLFVEQVRAGRPITITDPSMTRFMMTLDDAVELVLYAFEHGNN
                     GDIFVQKAPAATVEVLTRAILELMGRPDHEVRVIGTRHGEKLYEALLSREERAAAEDL
                     GGYFRVPPDGRDLNYGKFVEQGEVRISSAEDYNSHNTDRLDARGMRELLLKLEFMRRI
                     AAGEAAHPED"
     gene            complement(7876..8673)
                     /locus_tag="VF453_21780"
     CDS             complement(7876..8673)
                     /locus_tag="VF453_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HEX7690352.1"
                     /translation="MLRPRLIPCLLVHKGGLVKTRKFGAPKYVGDPINAVKIFNEKEA
                     DELMVLDIDAAVDKVEPNYALIAKLAAECRMPLCYGGGVTTAQQAARIIDLGVEKVAV
                     SSAAVADPGLLTRMTDALGAQSVVAVLDVRRKSGLFGKGYELCTHNARTPHRLDPVTF
                     AQQLQQAGAGEIVVNSIDRDGEMNGYDLELARQMRAALKVPLTILGGAGSLDHVGTLL
                     GACGVVGAAAGSLFVFKGQYRAVLINYPTRQQKDDLCRVALRAHDEN"
     gene            complement(8675..9289)
                     /gene="hisH"
                     /locus_tag="VF453_21785"
     CDS             complement(8675..9289)
                     /gene="hisH"
                     /locus_tag="VF453_21785"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX7690353.1"
                     /translation="MIRIVDYGVGNIQAFMTMFKRLGIDATRARTANELEGATRLILP
                     GVGTFDRAMTLLNESGMRPRLEEMVLESKVPTLGVCVGMQMLAAGSDEGSLPGLNWIP
                     GRVRALAGHPRSAHLPMPHMGWNDVHVRADHKLFTGFEPQPRFYFLHSYYFDAADSSD
                     VAATAEYGHHFDCVVSRGHIHGVQCHPEKSHHFGAQLLKNFAML"
     gene            complement(9286..11763)
                     /locus_tag="VF453_21790"
     CDS             complement(9286..11763)
                     /locus_tag="VF453_21790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HEX7690354.1"
                     /translation="MNSATHRPSFDDLRLVCDYLRGVRNEIALDVHTIGMAGKDPDLY
                     GKIPRPSRWLAKVRYLPFLSKVVHRVSLPVWHFLAPVLFNRQLRAMQGQRRTGQPAQF
                     DAGGQLIALSLRTLDIVHTRHIDPMPRQWLEFPWLRLGKRPEGVRTLAAASLLDDADL
                     RRSLALARLAHRVLPRRRGMSGWGLQSYTAWRWFFARIAVDKLPGPLLMIEHFDRWAV
                     LVDGSIQRSRQRQPVRRLTLMQHGSVNADVTPRGLGVALPTRLRAVNHLHVYSAADAQ
                     VFKEEILSPTLVERGIGVSFYRPSIALTDMCEPGIPSILFVGYPLCEAAHRALMRALR
                     DAGQWQLFYKPHPTNRASAGIALLPWIMVEGRSVFPRVDLIVSYPSTLVSEYAAHGIP
                     AVVHRMDVSPDQIVGRMSEILQITRGRGVPAATNICETAIHAAPSPTPPPMTLAPLRP
                     YRICGRCIMDTSDPNIRFDAQGLCEYCNNFDTTIKPNWHTDAEGERRLARLADTIRQA
                     GRGRDFDCIIGLSGGLDSSYSAYVAKEKMGLRPLLFHVDAGWNTDQAVGNIEKLVDGL
                     GLDLYTEVINWEEMKDLQVAFLKSGIPDQDLPQDAAFFSSLYKFARQYKIKHVITGSN
                     FSTECCREPEEWGGYLGIDTMLFHDIHRRFGKRPLETFPLKDILVYKLYYQNILGMKV
                     HHPLNLVPFIKKDAEDELDRRFGWQRFQHKHHESRFTRFYEDYWLPRKFGFHKRRAHF
                     SSLIVTGQMTREAALARIARPEMHEHFLKQEFEYVAHKLDLSVDDLQQIFDGPNKTYR
                     DYRNKRWMIGLGANAMRWLGLEKRYFR"
     gene            complement(11760..13193)
                     /gene="wzy"
                     /locus_tag="VF453_21795"
     CDS             complement(11760..13193)
                     /gene="wzy"
                     /locus_tag="VF453_21795"
                     /inference="COORDINATES: protein motif:HMM:NF025654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HEX7690355.1"
                     /translation="MRVERLHLLQVGILLTWLLLYVLDVALGMAEGACTVFLPVSLAA
                     LLLQQLRAERPPFDLSIVFLASCLFFFCIGIILWPVSSLPPTEFESLVEASFTMSELD
                     YASFLIGISIAITMLAMSVCRNWASYRVPLVFTPPPDPRARILYRLGVAFMVMSLPAV
                     VIESWMQFRYIQDAGYLALYVEGVPTSAWAIYFFYLFYFGFGLAFTFASTRRAFLGPA
                     TLYLVAATLDSLKGARGAVLVPLLFVAWYYCSRFDVKVRLGVVARNLALLGGLFVFMT
                     YQRDPELLSGGIGQFLVDALSTQGRSLQLTILYQQHADEIARYGNDMVLSNLLIPFNV
                     LLHPELRESAQSMDHVLYSNNLKHILTYVLNPDYYFAGGGTGGVYTVELIEAGAIMFI
                     ALSIGLGWMLAWLPGAMRRPWIRFLSIHLFATVFYLPRGEFFFNTLIVGKALFLYVLV
                     ICLGDVLRRVRGTAASRGTSTQQGTHA"
     gene            complement(13183..14472)
                     /locus_tag="VF453_21800"
     CDS             complement(13183..14472)
                     /locus_tag="VF453_21800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7690356.1"
                     /translation="MDEKLHIVVSSFSDCREKNGIVTFMDILRRNAGGFGDRGFRLAF
                     ANYVDFPSRPPARPGPVRADAPTPPAPPGAATASAPGRIRRTMLAVKYWIKGQLAKRP
                     LLAFLLMTATLSRRGLVTTLKARLRAGPGSVHFHQDAFCAYFGQWILHRSSRRILLLH
                     SGNDSLRQVFIHFHGMRGTRYEACVREAFRRTLAGLDGIVTLNERYADTLRAQFPHID
                     VRCIYNTSPFTGEDADRHRTARPAQEAPGRRIEILAVGSLQPIKGFDLLVGAAAGLPD
                     ADRDRLHVTIVGGGPARPALQAAVDAAGLREVITLNGESDHVASFLGRADAYILTSRD
                     EGFPIALIEASGFGLPIVATRVGAIPEVYDEASCLFMEPTVDSIREALAAIARGGVDL
                     DELARRSRAVFDAKLSLDAFMKAYCELFDQVGRRHAR"
     gene            complement(14465..15760)
                     /locus_tag="VF453_21805"
     CDS             complement(14465..15760)
                     /locus_tag="VF453_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690357.1"
                     /translation="MSLAGSTLLVMASRILVMFAGLIVSAVAAHSLSSVEQGYFFTFM
                     SLAAAQTLFELGITNLILHHLSHARAGILCARTAEGKRTAIALAMSTRDYSTRYFRRA
                     AGLFIVFVGTGGAAFFGLSRSASGAHWQSPWMLMLAGTALSLFNLSFYSYLEAFGRLS
                     VSYRVRMASTVILVVAFSVAAALGGGLLSYPLALLVANGCAFAVLRRSCMEVDHEHGL
                     TGAFDRQSIGIGREQRKMAASAVAGYVTANSLTPYAFHFFGPEIAGRFGLTMSIFTAI
                     AAVAMARTTAEAPTYGPLIAAGEWATLMARFRQTLFFSAGLALLLGILAIAGRRVGLE
                     FAPRYANRVLDTTGFVIVGLLIVANVTLSVTSTVLRAFKIERLMWPSLTAALVVLAAQ
                     LALRLDPAHCLALLAAFNGFVFFPYARHLLTTQIALSHG"
     gene            complement(15773..16477)
                     /locus_tag="VF453_21810"
     CDS             complement(15773..16477)
                     /locus_tag="VF453_21810"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEX7690358.1"
                     /translation="MAEPRLLAIIPARQGSKRLPGKNARPLGGRPLIAWSIDVALAAG
                     TFCDVLVTTDDEELATIGRDVGALVPWLRPAELATDTATSGDVLRHALAWYESHRGAV
                     DGVVLLQPTSPLRRVSSITGAVQRFLDQPIGAAPATVVSVSPSAVPPEWCFRIDGGAL
                     RPVLGWDEIGKRSQDLQAAYQLNGSIYVAGSDTVRSGRPLVAPGTLAFLMEGPEEAVD
                     IDTEDDWRIAESFLAR"
     gene            complement(16470..17525)
                     /locus_tag="VF453_21815"
     CDS             complement(16470..17525)
                     /locus_tag="VF453_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7690359.1"
                     /translation="MKNWEFALIRATSTLQEAMSALDRGGLQIAVVVDEDRRVTGTVT
                     DGDVRRALIRKVALEAPVSQAMNRQPRTARVGSSKASVLQLMERHQISQIPLVDAEGR
                     VVGIETLHGLLHGTRRDNAVFLMAGGFGTRLQPLTDNCPKPLLQVGDKPILEIIIENF
                     ARSGFHRFFISTHYRPEMIRERIGDGSRWDIDIQYVHEEEPLGTGGALSLLPADAIRE
                     PMFVMNGDLLTTLDANKLLAFHDRHQAIATMCVREHEHRVPYGVVESDGMRITSMVEK
                     PSYRYFINAGIYVLSPEILRGLAPGQRVDMPALLERQIAAGSAVNMFPVHEYWLDIGR
                     MEDFQRAQNEISGLFNG"
     gene            complement(17564..18565)
                     /gene="neuB"
                     /locus_tag="VF453_21820"
     CDS             complement(17564..18565)
                     /gene="neuB"
                     /locus_tag="VF453_21820"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEX7690360.1"
                     /translation="MKTLIIAEAGVNHNGDIELALELVRRAAQAGADLVKFQTFQTGR
                     SIARHAPKADYQKQATGATESQYEMVRKLELSADDHRRLLAECRRCGIGFFSTAFDRD
                     SHDLLIRLGVDRVKVPSGELTNLPLLRHMTREGLPVILSTGMATLGEIETALGIIEAN
                     GTPRTRITILHCTTEYPAPMHEVNLRAMQTLSAAFGTAVGYSDHTEGIEVPIAAVALG
                     ATIIEKHFTLDRSMPGPDHRASIDPDQFAAMASAIRNIEVALGDGIKAPTASELRNRL
                     IARKSIVAARPIRAGETFDESNLDTKRPGTGLSPAQWDEVVGRVAKRDFGEDELIEL"
     gene            complement(18562..19197)
                     /locus_tag="VF453_21825"
     CDS             complement(18562..19197)
                     /locus_tag="VF453_21825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEX7690361.1"
                     /translation="MTKLGIFGTSGFAREVGDIAEALGYDPIYLAADAVELAAWQFDA
                     PAILESELASLGTIPFAVGIGENRVRERVAMRHRDRLTFPSLAHPSATFGAGQRAKVE
                     AQRGVIVCAGVRFTNNIQVGQFSIFNLNATVGHDCRIDDFVNVSPGANISGNVHVGAR
                     AWIGTGAAINQGSNADKLRIGQDTVIGSGSVVVRSCDEQAVYVGIPAKRIK"
     gene            complement(19226..20374)
                     /gene="neuC"
                     /locus_tag="VF453_21830"
     CDS             complement(19226..20374)
                     /gene="neuC"
                     /locus_tag="VF453_21830"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEX7690362.1"
                     /translation="MRRRVAVVTGSRAEYGLLYWVLKELHASTEFDLQLIVTGMHLST
                     EFGMTVRQIEADGLPIARRVDMLLASDSAGAIAKSMALGLIGFSDALEALRPELLVVL
                     GDRFEILAAAEAALVHNVPLVHIGGGDTTEGAIDESIRHAITKMAHLHLVTNAGAARR
                     VEQMGEDPARVRIVGNPGLDHLRRTTLLDREALGQVLGHPLGARNLLVTFHPVTLEPG
                     AASRQQQMLLAALDRLPDAPTLWFTRPNADAGGRQLASELDAWAAERPLAHVFTSLGQ
                     QRYLSLMSHVDAVVGNSSSGLCEAPSFGIPTVDIGDRQRGRLAAASVIHCAATADDIH
                     AAICEAMRMDCSGTVNPYGDGESTARILSALRNFRPDGDVLKKRFRDL"
     gene            complement(20371..21519)
                     /locus_tag="VF453_21835"
     CDS             complement(20371..21519)
                     /locus_tag="VF453_21835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HEX7690363.1"
                     /translation="MFERLVDFIREQYGTREFIPLHAPVFRGREREFVLDTIESTFVS
                     SVGAYVDRFERETAAFCGSPRAVAVMNGTAALHIALLLAGVERDDLVVTQPLTFVATC
                     NAIAYCGAQPLFIDVDRRTLGLSPAALDAWLQEHALVDDAGDCRTREGHRRIRACLPM
                     HTFGHPVDLDALVAVCERWRLVLVEDAAESLGSYYKGRHTGNFGRIGVLSFNGNKIMT
                     TGGGGMLLADEEIGRRAKHLTTTAKIAHPYEFVHDEVGYNYRLPNLNAALGCAQLEQL
                     PAFLESKRALAARYIAFFQDSDLQPIVEPEHCRSNYWLNGVICDGPAQRDALLKATND
                     AGVMTRPIWTLMTRLPLYAHAPRGALDNAQWLEARVVNLPSSVLPGRP"
     gene            complement(21528..22508)
                     /locus_tag="VF453_21840"
     CDS             complement(21528..22508)
                     /locus_tag="VF453_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HEX7690364.1"
                     /translation="MKHVLVTGADGFIGSHLVELLAREGLQVRALSQYNSFNSWGWLE
                     DVDCLKDIEVVSGDVRDPHFCRKLTKGVDAVFHLAALIAIPYSYVAPDSYVDTNVKGT
                     LNICQAALDNGVARVIHTSTSEVYGTAQYVPIDEKHPLQPQSPYSASKIAADAMAMSY
                     FNAFNLPLTIARPFNTYGPRQSARAVIPTIITQIASGMRQIKLGDITPTRDFNYVEDT
                     CRGFLELARCGEAIGQTVNIGSNYEISVGDTLHLIKELMGSDVEFVTDQDRIRPGKSE
                     VFRLWGDNTRIRELTRFEPRVDIRAGLARTIDWLTRPANLARYKAHIYNV"
     gene            complement(22552..23655)
                     /locus_tag="VF453_21845"
     CDS             complement(22552..23655)
                     /locus_tag="VF453_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX7690365.1"
                     /translation="MQTSSHIDTTDDPNWLDLVLIVARRWRLVVASSLAGGVLAFLLS
                     MLITPQYTAKATFLPPQTSGGSGLGLALQSLGPLAGLATGMGTKTSGDLYVSLLESES
                     IADQMIDHFHLRQLYEVKYLVEARDALKSRTRVTLGKKDGLISIEVDDTDPQRAAAMA
                     GQYIEYLRQLTAGMALTEAQRKRVFFENQLQATREKLAQAQAALQASGFNPGALRSEP
                     TAATQEYAKVMGELTALEVRRSAMSTQMASSSPEMMHLDAAVSALRARLRAIEQQSTD
                     SAQSQDYVSKFREFKYQESLFEALSKQYEEARLDESKDDSLIQVVDRALVPEKKSKPK
                     RSRFAIAGLLLAAVLSSILCVAPRIRRLATERR"
     gene            complement(23754..25712)
                     /locus_tag="VF453_21850"
     CDS             complement(23754..25712)
                     /locus_tag="VF453_21850"
                     /inference="COORDINATES: protein motif:HMM:NF018010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX7690366.1"
                     /translation="MRRLASPHRRLSLLGGFALSIAAVLLQVPAHAQFGGGIPSLDAT
                     AKPGDGNASDIDFGNAGGSGSQANGVGGANSGTMRATTQLNQAGGNRGNRRDQPEPPA
                     LAASVPYEPSEFERYVQQQVDGKVRRFGSELMTDPAASLQTQDPLPSVPGDYIVRPGD
                     EIALNIWGSADANLRLTVDRAGGISVPRVGTITVGGVRNADLANVIRRQVAHVFTNFE
                     LTANLGQVRPIRVFVGGFAQRRGSLTVNGLSSVLHVLMRAGGPSAAGSFRDIHLMRGG
                     NEVASFDLYDLLLKGRRDADRLVQPDDVVFVGPVGKQVALLGSVNQPAIYELKSGETL
                     DDLLHMAGGFTAVADDTRVAVERLSDRETGRVSELALPAHRTEPLQNGEIVHAFSVVS
                     ASLPKLHQNERVRIEGEVLHPGDYILPPGSRMEDALRVAGGMTSAAYPYGTDFRRESV
                     RQAQQLNYDRALRDLETDLSKAAATTRATNAEELATQGAATTANARLLERLRQIKPTG
                     RVVLEIEPDDKALPDLALEDGDIINIPTRNSSVGVFGSVFNTGNFLFKPGHDVQQYLR
                     LAGGPTRDADKSSMFVIRANGSVLSARADASFWHSSDFLTAEVLPGDTVFVPEELSRS
                     SFVQDAKDWTQILYQFGLGLAGIKALGL"
     gene            25938..27107
                     /locus_tag="VF453_21855"
     CDS             25938..27107
                     /locus_tag="VF453_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7690367.1"
                     /translation="MSASTPTFLPFALPEIGDEEIAEVVDTLKSGWVTTGPKAKRFEA
                     AFTEFLGDPALESIAVNSATAGLHLALEALGIGPGDEVITTTHTFTATAEVVRYLGAD
                     VVLVDIDPATLNIDPAAVEAAITPRTKCIVPVHYAGLAADMGAILAIASKHGLRVVED
                     AAHALPTLSAGRLIGTLASDITVFSFYANKTMTTGEGGMLVTRDPELAKRAKVMRLHG
                     MSRDAFDRFTATKPSWYYEIVAPGFKYNLTDIAAALGLQQLKRLPAFQERREQLAARL
                     NAGLAGLPLVLPPTAPAGDRHSWHLYVVRLSDEAKVPRDEFIERLFKLGIGVSVHYIP
                     LHLQPYWRERYGLKPEQFPHSQRAYERMVSLPLYTRMTDADADRVCAAVREALQA"
     gene            27119..27715
                     /locus_tag="VF453_21860"
     CDS             27119..27715
                     /locus_tag="VF453_21860"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7690368.1"
                     /translation="MFKRAFDVVAALLGLALFGLPMLAVAAWIKLDSPGPVFFRQERV
                     GRGGRPFRIHKFRTMRVDAEARGPQITVGADARITRVGRILRDNRLDELPQFLDVLAG
                     DMSLVGPRPEVPRYVAVWPPALRERVLAVRPGITDPASLEFRDEASLLAAAADPEREY
                     VEVILPRKLARAADYADHASLWTDLAVIGRSVGVLFSR"
     gene            27759..29645
                     /locus_tag="VF453_21865"
     CDS             27759..29645
                     /locus_tag="VF453_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX7690369.1"
                     /translation="MNPSPIVPSPTFWHRLDRFLARLRPHREPLSLVVDGLLVVLAWN
                     VTYLFRLGFERWLEARPWYDPWVLLGIVFAYLGMFALLKVPQGMWRFSGFGEVKRLTW
                     ACLVAGVCSAAVVMGLQLYGVPRSVLALHPVVTLMAVCMVRIGYRMVYEHSRAQITGG
                     DGEVRRALILGAGEAAKRLMAGIHQQGWIVLGLLDDDPAKQGARFAGAPVLGPLEAVG
                     DKGVVGAATHIVIALPGASAQQRRRAFELAGATGLPVLTVPSADELRAGSGNIQRLRD
                     IEPEDLLGREPVRLDERGIDKVLHGRTVLITGAGGSIGSELCRQVARYEPARLVLFEL
                     SEFNLYSIEQELGQRFPGLPLVRLLGDVKDLDQLRATFARTKPDIVFHAAAFKHVPLV
                     EEQNAGAALRNNTLGTYHAALAAAENQVSRFVLISTDKAVNPTNVMGATKRAAEMVIS
                     SLVARFPGTRFMAVRFGNVLGSSGSVIPKFKEQIARGGPVTVTHPDIIRYFMTIPEAA
                     RLVVQAAAVGESGQVLVLDMGEPVRIVDLARQLIRLSGHAEDSIALEFTGLRPGEKLF
                     EELLADDDTTLPTAIARLRLARLGAQDGQALEELLRFARGEVGAMDDATVRTELRKVV
                     PEYK"
     gene            29803..30945
                     /locus_tag="VF453_21870"
     CDS             29803..30945
                     /locus_tag="VF453_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7690370.1"
                     /translation="MTFAAAFLVSFLLALAIVRSQGRHGRLSYDHDLSGPQKFHAAAV
                     PRIGGVGIFAGVLAAIFLVAAREPLDVDPMFVLLACAMPTLLSGLVEDFTKRVPPRWR
                     LVLTAMSAVLALLWLGARIERLDVHALQWLLLWPLEAAALTVFVVTGTTNAVNLIDGF
                     NGLASMSVAIMLSAIGLVAWWVHDALVCELAFAVVGAVLGFFAFNFPRGRIFLGDGGA
                     YFIGFMLVELGLLLLHRNPQVSAMFPLLVCGYPIFETIFTMYRRRFVQRTATGLPDAM
                     HLHHLVFRRVSRRAFDRQGRPRSLTWRNSATSPYLWMVCACAAVPAVCFWNDTYVLLA
                     FIGVFMLGYVALYRRLDPRLERALAAKADPSSAELRIAHTLTGTED"
     gene            31244..31927
                     /locus_tag="VF453_21875"
     CDS             31244..31927
                     /locus_tag="VF453_21875"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7690371.1"
                     /translation="MAPGILHRRAHAAGGSMTPIGLHPLEHALKRIFDLASVVVILAL
                     FGWLMVIIALLIRMNGRSVIFGHERVGRGGRTFQCYKFRSMVPNSQQVLQELLERDPQ
                     ARIEWERDFKLKDDPRITRIGRFIRKTSLDELPQLWNVVKGEMSIVGPRPVVQEEFDL
                     YYGHAREHYLSVPPGLTGLWQVSGRNDLDYEQRVELDKRYVESWNVFSDFLIVMRTVK
                     VMVARRGAY"
     gene            32024..32404
                     /locus_tag="VF453_21880"
     CDS             32024..32404
                     /locus_tag="VF453_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HEX7690372.1"
                     /translation="MIEQSSSPGRDEPAQDAHWRLLSLIEQHPEYSQRRLAEAMGVSL
                     GKTHYLLKALFEKGLVKAGKFTRNPTQMKYVYALTPAGMKHRMQLTREFLQRKEREYV
                     ALRHEIERLRTELAVDGRNSEPNP"
     gene            32433..>33195
                     /locus_tag="VF453_21885"
     CDS             32433..>33195
                     /locus_tag="VF453_21885"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7690373.1"
                     /translation="MSTVTLSNGTRFDAEAGATILDAALARGVVLEHGCRTGRCGSCK
                     ARVVHGETAPLRSPVSLADDEAARGWVLTCTHEARSDVALDIEDLGALAGIEARVQPA
                     RIDALESLAPDVVRVVLRLPPRAPLRFLAGQYIDVTGPNGVRRSYSIASDAAAGDRLE
                     LQVRRVDGGVLSAYWFEAARANDLLRFHGPRGTFFLRPVAELHLVMLATGTGIAPLLA
                     MLRQVAAMPEGERPLSVSLYWGGRHGVDHYLDPRDA"
BASE COUNT         5146 a        11055 c        11589 g         5405 t
ORIGIN      
        1 cgtgggcatt gcggctcgcg ctcgtcgggc aggtcgcctt cgtcgcggcg ctcgcggccg
       61 gcctgcggct gggcgggctc gccggtgcgg gctggggggt gtcggcggcg atgacgctgt
      121 acttcggctg gtatttcctg aagctcgcga cctggccgct cgcgccggaa tcgacatcga
      181 acgaggacac cccgacatga cgctgcgcgc gacctggaac cgctggcgcc gcgccctgct
      241 gcggcgctgg gcgtggcagc ccgtgttcgg cgagcgctcg caccgcggcg cgttcctgaa
      301 gcacacgcgc atctcgggcg cgaccgtcat cgagcacgag gaccgcctgg cgctcggcga
      361 caacgtcttc atcggccact tcaacttcat cgaggcgagc ggcggcatca cgatcggcga
      421 gggcgtgcag gtcacgagcc actgcgcgat cgtcacgcac gcgtcgcacc gctcgcagcg
      481 cctgctcggc ccggcctacg tcacctggcc gctgaccccc ggcacgcagc gcccgggctg
      541 gatcgccggc cccgtgcaca tcggcgccta cagcttcatc ggcccgcaca gcctgatcga
      601 ggccaacacg cgcatcggcc gcggctcgct cgtgtgcgcg ggcagcttcg tgcgcggcga
      661 gtacccggac ttcgcgatcc tcgaaggccg ccccgcgcgc gtcgtcggcg acacccggcg
      721 cgccgacgag cggctgctcg agcgctaccc ggagctgcgc gtgctctacg acgcatgggc
      781 gcaggcgccc gagccgtcgc cgttcgggga gccgtgatga ggcgcgtcgc gacatcgccc
      841 gaccggagcg cgcgcgcatg acgcccgagt cgtcgcgtcc gctgcgcctc gtcctgtggg
      901 gcgacggcga gagcccgcac ctcgtgaagt gggcgcgcgc gctggcgtcc agggtcgagc
      961 tgttcgtcgc ctccagccgc ggcctcgcgc cgggcatcga ggcgctggtc gccccggagc
     1021 ggcggctcgc gctcggccag gcgacgaagt tcgcgggcgg caacgcggcg ctgctgaaga
     1081 cgctgccgcg cttcgccgcc tgggtgcgtc gggtcgatcc cgactggatc aacgcgcact
     1141 acctgagctc gcacggcacg atggcctggc tcgcgcgaaa gctcggcgtg cgcgcgcggc
     1201 tggcggcgtc ggcctggggc tcggacatcc tcgtggcgcc cgagcgcagc cgcgcgatgc
     1261 gctgggtcac ggggcgcgtg ctgcgcgacg ccgcgatctc gaccagcgac tccgcccaca
     1321 tggcgcagcg catgcgggcg ctcggcgcgc gcgaggtcat gaccttcccc ttcggcctcg
     1381 acgcgctgcc gccggtcgac ccggcgaccc ccaaggatcc gcgcctgttc ttcgccaacc
     1441 gcgggctcga ggacatctac gcgccgctgc gcgtgctgtc gctgttcgcg agcgcggccg
     1501 cggcatggcc cgacgcgcgg ctggtcgtcg ccaacgacgg ctcgatgcgc gaggccatgc
     1561 tgcggcgcgc gcggcaactg ggcatcgccg accgcgtcga gttcacgggc cgcctcgacg
     1621 ccgccgccca ggcgcgcttc tacgcccgcg cccgctggta cctgagcgtg ccgcgcagcg
     1681 actcggtctc ggtctccgtg ctggaggcga tggcccatgg ctgcctgccc atcctgtcgg
     1741 acctgccggc caaccgcgag ctcgtgcgtg ccggccacaa cggcgtgatc gtcgagcccg
     1801 agcgcggcga cctcgagctc gagtcgctgc tggtcgtgga cgcccgcgcc gacgcgatcg
     1861 ccgccctcaa ccgcgactgg gtcggcgcgc acgggctgtt cgggccgagc gtcgacgcct
     1921 tcgtcgcgcg gctggcccag ctttcaggcc gcgcctagcc gccgcggaca tacccgcgaa
     1981 cgaacctcgc catcccccgc gacgcccctg cgcgcccgca catgaacatc gtctacatcg
     2041 aacactacgc cggcacgccc gcgctcggga tggagttccg cccgtactac ctcgcccgcg
     2101 aatgggtgcg cgccggccat cgggtgcgga tgctcgcggc gtcctactcg cacgtgcggg
     2161 cgcggcagcc cgcggtcggc acgacggcgc tggacgagcg catcgacggc atcgactacc
     2221 gctggtaccc cacgcccgcc taccagggca acggtctcgg ccgcgtgaag aacatcttct
     2281 cgttcctgcg ccaggtgtgg gccgacacgg gccgcctcgt gcgcgaggcg aggccggacg
     2341 tcatcatcgc gtcgagcacc tacccgatgg acatctgggt cgcgcgccgc ctggcgcgcc
     2401 gcgcgggcgc gaagctcgtc ttcgaggtgc acgacctgtg gccgctgtcg ccgatcgagc
     2461 tgtccggcat gtcgcccagg cacccgttcg cgcgcctgtg ctcatgggcc gaggccgacg
     2521 cgtaccgcga ctcggacgtc gtcgtctcga tgctgccctg cgtgcacgag tacatggcct
     2581 cgcgcggcct ggacctgcgc aagctggcga tcgtcccgaa cggcatctcg cccgacgact
     2641 gggccgcagc gcccgcaccc ttgcgcgagg acgtcgccgc cgcgctcgat cgcgcgcgcg
     2701 aagccggcga aacggtggtg gggtatgcgg gctcgatggg cgagcccaac gcgctcgaca
     2761 cgctgctcga cgccgccgcg ctgctgcgcg acgacgccgc cgccgccggc cgacgcatcc
     2821 gcatcgtgat ggtcggcgac ggccacctgc gcgcccacct ggccgcgcgc gtccaggccg
     2881 agggcctggc caacgtcacg atgctcccgc ccatccccaa gccgcaggtg cccgcgctgc
     2941 tcgcgcaggt cgacatcgcc tacatcggct ggcagcgcgt acccatctac cgcttcggca
     3001 tcgcgcccaa caagctgatg gactacatga tggccgggtg cgcggtgctg cactcggtgg
     3061 aagccggcaa cgatccggtg gcggaggcgg gctgtgggat cacggtcgcg ccgggcgatg
     3121 cgcgggcggt ggccgatggg ctgttgcgcc tggcggcact gccggcgggc gagcgcgccg
     3181 cgatgggggc gcgggggcgg gcgttcgtgg aggcgaagca cgcttacccg gtgctggcgg
     3241 cgaggttcat cgaggcggtg gaggactgcg cgagcattcc ggcggcaacc tcaggacggg
     3301 cctgaacgcg cccagcctgg tgtggccgag atggttcctc gcaagagcgc agtcagtcgg
     3361 aggcagggtt ggccagccag gcctcgaggc gagacatcag cgtggctcgc ttgaaattct
     3421 cctcgccgta gcgctgccct tgcgccccca tctgcgcacg ctcgcccgcg gaaagatccg
     3481 cgagtgcgat ggccagtcgc gccagctcgg cggcgttgcc ggacggggcg acgaagccgg
     3541 cgcgcgcttc ctcgatgacc cgggcgcctt cgccatcgag catgcccagc aggggcttgc
     3601 cggaggcgag atagctctgc accttgccgg gaatcgtcat cgcgaagatc ggatccgctt
     3661 tgagcgacac gagcagcgcg tcggcaccgg cgaaaaagga cggcatgcgt tcgaccgggt
     3721 gccggccgag catgaatacg gtcgcggcca ggcccaggcg atcgatgtcc gccctgaccc
     3781 ggggcgcggc gcggccgtca cccacgatga gccagtggac gtccgggcgg tggcgggtca
     3841 ggcgggcagc ctcgagcacg gccgggaagt cctgggcgtc gcctatgttg cccgcgaaga
     3901 cgattttgaa gctgcccgcg tggggagcca gctcggcggc gagggcgtcg gcctgcgccg
     3961 ccgcgtcgaa gatctcctcc gaccaggccg ggaaatagcg gaacctccgc gaggatcctt
     4021 cccagcgcct gatcggctcg acgaaggcgc gggactggcc gaggatcagg tcgcatcggc
     4081 ggtagatgaa ccggcacagc gcgccggcca cgcgaagcga gaactccgac ttcaccatgc
     4141 cgactgcctt gagcgtgtcg ggccagaggt ccaggaccca catcgacagc ggggcacgct
     4201 tgatccgctt cagcaggatc gcgggcaggg cggacgtgat gggcgaggtt tcgaagacga
     4261 agatcgcgtc gaacgggcgg ccccgcagca gccaaggccc gagggcgcag ccccagaaca
     4321 cgaagctcca gtaattgagc agcaagctca ggttgccctt gccccgtggg cgcagcggca
     4381 cccgcagtac ggatgcaccg gcgtatgcgg cgaatcgctg cggcgcggca gcgaaatcgg
     4441 gtgagacgct gccctccggg tagttcgggc gccccgttag gacagtcacc tcgtggccgc
     4501 gcgaggtcag ctcagagacg atctcgttga cccgaaaggt ctccggccag aagtactggc
     4561 tgacgacgag gaccttcacg cgggccctat tcgtagtcgc gccagacgac gcgcttcacg
     4621 tagtcggtat agctgtggat gatgcgcacg accttgtcgg agacgttggg catgccgtag
     4681 tccgcgatcc ggcgcagcgc gcgctcacca ccgcgaggct gtcgttcgag gatcgccagt
     4741 gcctgtcgaa tgcgctggag ctccaggccg accatcatca ccgccgcctc ttccatgcct
     4801 tccggccgct cgtgcgcctc gcgcaggttg agcgctggga agttgaggat cgacgactcc
     4861 tctgtaatcg tgccgctgtc cgacagcacc gcgcgcgcat gcatctgcag cttcacatag
     4921 tcgtggaacc cgagtggctt gagaagccgg acattcgcgt ggaagcgcac gcccatcgcg
     4981 gtgatgcgct tctgggtgcg tggatgagtg gagacgatga ccggaagccc gtgatcctca
     5041 gccagaccgt cgagcaccgc gcagagcttg ccgaagctgc ggtccgattc gatgttctcc
     5101 tcgcgatgcg cgctgaccac gaaataacgc ccatccgcca ggcccaggcg cgacagcgcg
     5161 tccgaggcgt cgatcctgtc acggtagtgc gagagcacct cgagcatcgg gctgccggtc
     5221 ttgatgacca tgtctggggg cagtccttcg cgcaggaggt attcgcgggc gatgtcgctg
     5281 tacgtgagat tgatgtccgc ggtgtggtcg acgatgcgcc ggttggtctc ttccggcacg
     5341 cgcaggtcga agcaccggtt gcccgcctcc atgtggaaga tcgggatcct tcggcgcttg
     5401 gcggggatga ccgctaggca gctgttggtg tcgcccagca cgagcagggc gtcggggcgc
     5461 tccttggcga gcgcgtcgtc gaccgcgatg atcaggtggc cgatcgtgtg cgcggcgttg
     5521 gaaccggcgc tgccgaggaa gtgatccggc ttgcggacgc ccaggtcgtc gaagaagacc
     5581 tggttgagct cgtagtcgta gttctgtccc gtgtgcacca gcacgtggtc gcagaactcg
     5641 tccaggcgcg cgaggacgcg cgagaggcgg atgatctccg ggcgggtgcc gacgacagag
     5701 acgactttca gtttcttcat gcttgcctga ccttgcgcgc gaccgtgtcc ggcctttgcc
     5761 ggtcgaagat ctcgttggcc cagaccatga cgaccagttc gtcgtctccg atgttggtca
     5821 cgttgtgcgt ccagccgggg atggtctcga cgatccggcc ttcgccgcca cgggcgacga
     5881 tctcgtgggt ctcgttggtc acgatatggc ggtagccgaa gctcgccgtg cccttgatca
     5941 ccaggaattt ctcggtcttg gtgtggtggt agtgctcgcc gcgggtgatg cccgggaagg
     6001 ccgtgaagta gccgaactgg ccgcagtcgg gcgtcttcag catctccacg aacacgccgc
     6061 gcgggtcggc gtggcgagcc acctcgtact cgaaggcggg cagcggcagg aagctcacgt
     6121 acgtggcgta gagcgcgcgc gccaggccgg tgcccacgcg ctcggtcacc agcgattcgc
     6181 ggctggccgc gaacgcgcgg atctggtcgg ccagctcgcc gaccgtcgta tcgtattcgg
     6241 ggccggcgga cagcagtccg gacggcgtgt ccgcggcgtc gagcagcgcg cagaaatggc
     6301 gcacgacatc gtccacgtag accagccgca gcggtgcgtc cgccttgttg acgacgatcg
     6361 gttcgccgcg cgcgatgttg tggcagaagg tggcgacggc cgagttgtag ttcggccgcg
     6421 accacttgcc gaacacgttg gtcagccgat agatatggac gcccgcaccg gtgtgtcggc
     6481 cataggagaa cagctcgtct tcggcctggc gcttgctacg gccgtaggga ttgtcctgcg
     6541 tcgcctgggt ggaagacgcg aagacgacgc gaggaggccg gggctgggcg gccatcgctt
     6601 cgcacaggcg ccgagtgaag tcatggttgc ccgtggcgaa ctcggtcggg tcctgcgggc
     6661 ggttgacgcc cgcgaggtgg aacacgacgt ccacgtcggc gagcagcgcg gacagcgccg
     6721 gcacctccgc gtcgtgggga accagcagcg ggtcgccatg acccgattcc tgcagccgca
     6781 cctgcaggtt gcgtccgatg aatccgtgtc ctccggtgat tgcgatccgc atctcaatcc
     6841 tccgggtgcg cggcttcgcc ggccgcgatg cggcgcatga actccagctt caggagcagc
     6901 tcacgcatac ctcgcgcgtc gaggcgatcg gtgttgtggg agttgtagtc ctcggcgctg
     6961 ctgatacgca cctcgccttg ctccacgaat ttgccgtagt tcaggtcgcg cccgtccggc
     7021 ggcacgcgga agtatccgcc cagatcctcc gcggcagcgc gttcctcccg gctgagcaac
     7081 gcttcataga gcttctcgcc gtggcgcgtg ccgatcacgc ggacctcgtg gtcgggccgt
     7141 cccatcagtt ccaggatcgc ccgcgtgagc acctccaccg tcgccgcagg ggccttctgc
     7201 acgaagatgt cgccgttgtt gccgtgctcg aacgcgtaca gcacgagctc caccgcatcg
     7261 tccagcgtca tcatgaagcg cgtcatgctc ggatcggtga tggtgatcgg cctgccggcg
     7321 cgtacctgct cgacgaacag tgggatgacc gagccgcgcg acgccatgac attgccgtag
     7381 cgcgtgccgc agatgacggt gcgcgagccc tcgaggttgc gcgaagcggc caccatgacc
     7441 ttctccatca tcgccttgga gatgcccatc gcgttgatcg ggtagacggc cttgtccgtg
     7501 ctcaggcaga cgacgcgttc gacgccggcg cggatggcgg cttccagcac gttctcggtg
     7561 ccgagcacgt tggtgcggac cgcctccatc ggatggaact cgcaggacgg cacctgcttg
     7621 agcgcggcgg catgaaagca gtagtcgacc ccgcgcatcg ctgtctcgac cgtgcgcgcg
     7681 tcgcggacgt cgccgatgta gaacttcagc ttggggtcgt cgtagcgctt gcgcatgtcg
     7741 tcctgcttct tctcgtcgcg ggagaagatg cggatctcgc gcaggtcgga gtgcaggaac
     7801 cggcgcagca cggcattgcc gaagcttccg gtgccgccag tgatgaggag cgtcttgtcg
     7861 tggaacatga tccggtcaat tctcgtcgtg agcgcgcagc gcgacccggc agaggtcatc
     7921 cttctgctgt cgggtggggt agttgatgag cacggcgcgg tactgtccct tgaaaacgaa
     7981 caggctgccg gccgctgcgc cgacgacgcc gcacgcgccg agcagcgtgc ccacgtggtc
     8041 gagggaccct gcgccgccca ggatggtcag cgggaccttg agcgcggcgc gcatctgtcg
     8101 tgcgagctcg aggtcgtagc cgttcatctc gccgtcgcgg tcgatggaat tgacgacgat
     8161 ctcgcccgcg ccggcctgct gcagttgctg ggcgaaggtc acagggtcga gcctgtgcgg
     8221 tgtcctcgcg ttgtgggtgc acagctcata tcccttcccg aagagcccac tctttctgcg
     8281 cacgtcgagc accgccacca cgctttgcgc accgagcgcg tcggtcatgc gcgtcagcag
     8341 cccggggtcg gcgacggcgg cggagctcac ggccaccttc tcgacgccga ggtcgatgat
     8401 gcgcgccgcc tgttgggcgg tcgtcacgcc gccgccgtag cacagcggca tgcggcactc
     8461 cgcggccagc ttggcgatca gtgcgtagtt gggctcgacc ttgtccacgg cagcgtcgat
     8521 gtccagcacc atcagctcgt cggcctcctt ctcgttgaaa atcttcaccg cgttgatggg
     8581 gtcgccgacg tacttcggcg caccgaactt gcgcgtcttg accaagccgc ctttgtggac
     8641 gagcaggcag gggatgaggc gggggcgcag catgtcagag catcgcgaag ttcttcagga
     8701 gctgcgcacc gaagtggtgg ctcttctccg gatggcactg gacgccgtgg atgtggccgc
     8761 gcgagacgac gcagtcgaag tgatggccgt attcggctgt ggcggctacg tcgctgctgt
     8821 ccgccgcgtc gaagtagtac gagtggagga agtagaagcg cggctgcggc tcgaagccgg
     8881 tgaacagctt gtggtccgcc ctgacgtgga cgtcgttcca gcccatgtgc ggcatgggca
     8941 ggtgcgcaga tcgcggatgg ccggccagcg cgcgcacccg tccggggatc cagttcagcc
     9001 cggggagcga gccctcatcg ctgccagcgg ccagcatctg catgccgacg cacacgccca
     9061 gggtcggtac cttgctctcg agcaccattt cctccaggcg cgggcgcatg ccggactcgt
     9121 tgagcagggt catcgctcga tcgaacgtcc cgacgcccgg caggatgagg cgcgtcgccc
     9181 cctcgagctc gttcgccgtc cgcgcccgcg tggcgtcgat gcccagccgc ttgaacatcg
     9241 tcatgaacgc ctggatgttg ccgacgccgt agtcgacgat gcgaatcatc ggaaatatct
     9301 cttctcgagg cccagccagc gcatcgcgtt ggcgcccagg ccgatcatcc agcgcttgtt
     9361 ccggtagtcc cgataggtct tgttcgggcc gtcgaagatc tgctgcaggt catccaccga
     9421 caggtcgagc ttgtgcgcga cgtattcgaa ttcctgcttg aggaagtgct cgtgcatctc
     9481 cggccgggcg atgcgcgcga gggcggcctc gcgggtcatc tgcccggtca cgatcaggct
     9541 ggagaaatgg gcgcggcgct tgtggaagcc gaacttgcgc ggcagccaat agtcctcgta
     9601 gaagcgggtg aagcgcgact cgtggtgctt gtgctggaag cgctgccagc cgaatcgacg
     9661 gtcgagctcg tcctcggcat ccttcttgat gaagggcacg aggttcaacg gatgatgcac
     9721 cttcatgccg aggatgttct ggtagtacag cttgtagacc aggatgtcct tgagcggaaa
     9781 ggtctccagc ggacgcttgc cgaaccgacg gtggatatcg tggaacagca tcgtgtcgat
     9841 gccgaggtag ccgccccatt cctcgggctc ccggcagcac tcggtcgaga agttcgagcc
     9901 ggtgatcacg tgcttgatct tgtactggcg cgcgaacttg tacagcgagg agaagaacgc
     9961 cgcgtcctgg ggcaggtcct ggtcggggat gccggacttc aggaaggcga cctgcaggtc
    10021 cttcatctcc tcccagttga tgacctcggt gtacaggtcg agtccgaggc cgtccaccag
    10081 cttctcgatg ttgcccaccg cctggtcggt gttccatccg gcgtcgacgt ggaacagcag
    10141 ggggcgcagg cccatcttct ccttggcgac gtaggccgag taggagctgt ccagcccgcc
    10201 gctcagcccg atgatgcagt cgaagtcccg gccccggccg gcctggcgga tcgtgtccgc
    10261 cagccgggcg agccggcgct cgccttcggc gtcggtatgc cagttgggct tgatcgtcgt
    10321 gtcgaagttg ttgcagtact cgcacaggcc ctgcgcgtcg aaccggatgt tgggatccga
    10381 cgtgtccatg atgcagcgac cgcagatgcg gtagggcctg agtggggcga gggtcatcgg
    10441 gggtggtgtc gggctgggag cggcgtgtat tgccgtttcg caaatgttag ttgccgcagg
    10501 tacgccgcgc ccccgagtta tctgaaggat ctctgacatt cggccgacga tctggtcggg
    10561 cgagacgtcc attcggtgca ccacggcggg aatgccgtgc gcggcatact ccgacaccaa
    10621 cgtcgaggga taggaaacga tcaggtcgac gcgtggaaac accgagcgcc cctccaccat
    10681 gatccacggc aacagcgcga tgcctgcgct ggcccggttc gtggggtggg gcttgtagaa
    10741 cagttgccac tggcccgcgt cgcgcagggc tctcatcagc gcgcgatggg cggcctcgca
    10801 caagggatat ccgacgaaca ggatcgacgg gatccctggt tcgcacatgt cggtcagcgc
    10861 aatgctcggc cggtagaagc tcacgccgat gcctctttcc accaacgtcg gcgacaggat
    10921 ctcctccttg aacacctgcg catccgcggc cgagtagacg tgcagatgat tcacggcgcg
    10981 aaggcgcgtc ggcagggcca ctcccaggcc tcgcggcgtc acgtcggcgt tcaccgagcc
    11041 gtgctgcatc agcgtcagcc ggcgcaccgg ctgacgctgc ctggatcgct gaatcgatcc
    11101 atcgacgagc accgcccatc gatcgaagtg ttcgatcatc aggagcgggc cgggcagctt
    11161 gtccacggct atccgtgcga agaaccagcg ccaggcggtg tagctctgca agccccagcc
    11221 cgacatgccc cggcggcgcg gcaggacgcg atgcgccagc ctggcaaggg ccagggatcg
    11281 ccgcaggtcg gcgtcgtcca gcaggctcgc cgcggcgagc gtgcggacgc cttccgggcg
    11341 tttaccgagc cgcagccacg ggaactccag ccactggcgc ggcatcgggt cgatgtggcg
    11401 tgtgtggacg atgtccagcg tccgcagcga aagcgcgatc agctggccgc cggcatcgaa
    11461 ttgggccggc tgcccggtcc tgcgctggcc ttgcatcgcg cgaagctgcc gattgaagag
    11521 caccggcgcc aggaagtgcc acaccggcag gctgactcga tgaacgacct tcgagagaaa
    11581 gggaaggtac ctcaccttgg cgagccagcg gctgggccgc ggaatcttgc cgtacagatc
    11641 cggatccttg ccggccatgc cgatggtatg cacgtccagg gcgatctcgt tgcgcacgcc
    11701 ccgaagatag tcgcagacca ggcgcagatc gtcgaagctg gggcgatggg ttgccgaatt
    11761 catgcgtgcg tcccttgctg cgtggaggtc ccgcgcgatg cggcggttcc ccgaacgcgc
    11821 cggaggacgt ccccgaggca gatgacgagg acgtagagga acaatgcctt gcccacgatc
    11881 agcgtattga agaagaattc gccgcgcggc aggtagaaga cggtggcgaa gagatggatg
    11941 gacaggaaac gtatccacgg acgtcgcatg gcgccgggta gccaggccag catccagccg
    12001 agaccgatcg agagggcgat gaacatgatc gcgccggcct cgatgagctc gacggtgtag
    12061 acgccaccgg ttcccccgcc ggcgaagtag tagtcggggt tgagcacgta ggtcaggatg
    12121 tgcttgaggt tgttcgagta aagcacgtgg tccatggact gcgcggactc gcgcaattcc
    12181 ggatggagaa ggacgttgaa gggaatcagc aggttggaca gcaccatgtc gttgccgtat
    12241 cgcgcgatct cgtcggcatg ctgctggtag aggatcgtca gctgcagcga gcgcccttgc
    12301 gtcgacagcg cgtcgaccag gaactggccg atgccgccgg acaggagctc ggggtcgcgc
    12361 tggtaggtca tgaagacgaa tagccccccc agcagcgcga ggttgcgcgc gacgaccccg
    12421 aggcgtacct tcacgtcgaa gcgcgagcag tagtaccagg cgacgaacag cagcggcacc
    12481 aggacggcgc cccgggcacc cttcagcgaa tccagtgtcg cggcgaccag gtacagggtc
    12541 gccgggccga gaaaggcccg tcgcgtcgaa gcgaacgtga acgccagacc gaacccgaaa
    12601 tagaacaggt agaagaagta gatcgcccac gcggacgtcg ggacgccctc gacatagagc
    12661 gccaggtagc cggcgtcctg gatgtaccgg aactgcatcc aggactcgat caccacggcc
    12721 ggcagcgaca tcaccatgaa cgcgacaccc aggcgataca ggatgcgcgc gcgcggatca
    12781 gggggcggcg tgaagaccag ggggacgcgg taagacgccc aattgcggca gaccgacatc
    12841 gcgagcatcg tgatggcgat cgaaatgccg atcaggaacg aagcatagtc gagctcgctc
    12901 atcgtgaacg acgcctcgac gaggctttcg aattccgtgg gcggcaggga cgagaccggc
    12961 cacaggatga tgccgatgca gaagaagaag agacagctgg cgaggaagac gatcgacagg
    13021 tcgaatgggg gacgttcggc cctcagctgc tgcaacagca gcgcggcgag ggagaccggc
    13081 aggaagacgg tgcaggcgcc ctcggccatg ccgagcgcga cgtcgagcac gtagagcagg
    13141 agccaggtca gcaggatgcc gacctgcagc aggtgcaggc gctcaacgcg catggcggcg
    13201 cccgacctgg tcgaagagct cgcaatacgc cttcatgaag gcgtccagcg acagcttggc
    13261 gtcgaagacg gcgcgcgatc ggcgcgcgag ttcgtcgagg tcgaccccgc cccgggcgat
    13321 ggcggccagc gcctcgcgga tgctgtcgac cgtcggctcc atgaacaggc aggaagcttc
    13381 gtcgtagacc tccgggatcg cgcccacgcg ggtggcgacg ataggcaggc cgaagccgct
    13441 tgcctcgatc agtgcgatcg gaaaaccttc gtcgcggctg gtcaggatgt aggcatcggc
    13501 gcggccgagg aacgacgcga cgtggtccga ctcgccgttc agcgtgatga cctcgcgcag
    13561 tcccgccgcg tcgacggcgg cctgcagcgc gggacgggcg ggaccgccgc cgacgatcgt
    13621 gacatgcagg cgatcacgat ctgcgtccgg caggcccgcg gcagcgccga cgagcaggtc
    13681 gaagcccttg atcggctgca gggatcccac ggccaggatc tcgatgcggc ggcccggcgc
    13741 ttcctgcgcc ggccgggccg tgcggtggcg gtcagcgtcc tcgcccgtga acggcgacgt
    13801 gttgtagatg cagcggacgt cgatgtgcgg gaattgtgcg cgcagggtat cggcgtatcg
    13861 ctcgttgagc gtgacgatgc cgtcgagccc ggcgagggtg cgccggaacg cttcccggac
    13921 gcacgcttcg taccgcgtcc ctcgcatgcc gtggaagtgg atgaagacct ggcgcaacga
    13981 atcgttgccg gagtgcagca gcaggatccg gcgcgaactg cggtgcagga tccattggcc
    14041 gaagtaggcg cagaacgcat cctggtggaa gtgcacgctg ccggggcccg cgcgaaggcg
    14101 ggctttgagg gtggtcacca ggccgcgccg cgacaaggtc gccgtcatga gcaggaacgc
    14161 gagcagagga cgcttcgcga gctgaccctt gatccagtac ttgaccgcca gcatcgtgcg
    14221 ccggatccgt cccggcgccg aggcggtcgc cgcaccgggc ggggcgggcg gggtcggggc
    14281 gtcggcccgg acgggtccgg ggcgcgcagg tggccgcgac gggaagtcga catagttcgc
    14341 gaacgcgagc cggaagcccc gatcgccgaa tccgccggcg ttgcgccgaa ggatgtccat
    14401 gaaggtgacg atcccgttct tctcccggca gtcgctgaac gaggagacga cgatgtgcag
    14461 cttctcatcc atggctgagg gcgatctgcg tggtcaggag atgccgcgca tagggaaaga
    14521 acacgaagcc gttgaacgcg gcgagcaatg ccaggcagtg ggcaggatcg agccggagcg
    14581 ccagctgcgc cgccaggacg acgagcgccg ccgtgagcga aggccacatc aatcgctcga
    14641 tcttgaatgc ccgcaggacc gtactggtca ccgacagcgt cacgttggcg acgatcagca
    14701 agccgacgat gacgaacccc gtcgtgtcga gcacgcgatt cgcgtagcgt ggggcgaatt
    14761 ccagccccac tcgcctgccg gcgatggcga ggatgcccag cagcaatgcc agcccggccg
    14821 agaagaacag cgtctgccgg aaccgggcca tcagcgtcgc ccattcgccc gcggcgatca
    14881 acggcccata ggtcggcgcc tcggccgtgg tgcgcgccat ggcaaccgcg gcgatggccg
    14941 tgaagatcga catcgtcaac ccgaaccggc cggcgatttc ggggccgaag aaatggaagg
    15001 catagggcgt cagcgaattg gcggtgacat acccggcaac cgccgaagcc gccatcttgc
    15061 gctgctcgcg gccgattccg atcgactgcc ggtcgaaagc gcccgtgagg ccgtgctcgt
    15121 ggtcgacctc catgcagctg cggcgcagga ccgcgaaagc gcagccgttg gcgaccagca
    15181 gggccagcgg gtacgagagc agtccgcctc cgagtgcggc cgccacgctg aaggcgacga
    15241 cgaggatgac ggtcgaggcc atccggacac gataggaaac actgaggcgc ccgaacgcct
    15301 cgagatagga ataaaagctg agattgaaga gactgagcgc ggtgcctgcc agcatcagca
    15361 tccacgggga ctgccagtgc gcccccgacg cgctcctgga caggccgaag aatgcggcgc
    15421 cgcccgtccc cacgaagacg atgaacaggc ccgccgcgcg tcggaagtag cgcgtcgaat
    15481 agtcgcgcgt cgacatcgcc agcgcgatcg cggtgcgctt gccctcggca gtgcgtgcgc
    15541 acaggatccc tgcccgcgca tgggacaggt ggtgcagtat caggttggtg atgcccagct
    15601 cgaagagcgt ctgcgcggcc gcgaggctca tgaacgtgaa gaaatagcct tgctccacgc
    15661 tcgagaggct gtgcgcggcg acggccgaca cgatcagtcc ggcgaacatc accaggatcc
    15721 ggcttgccat gaccaggagg gtggacccgg cgagggacat gtcgactccg ggtcagcggg
    15781 ccaggaacga ttcggcgatg cgccagtcgt cctcggtatc gatgtcgacg gcttcctccg
    15841 gcccctccat caggaaagcc agggtgccgg gcgcgacgag gggcctgccg ctgcgaacag
    15901 tgtcgctgcc ggcgacgtag atcgagccgt tcaattgata ggccgcctgc agatcctggg
    15961 accgcttgcc gatttcgtcc cagcccagca ctggccgcag cgcgccaccg tcgatgcgga
    16021 aacaccattc cggaggcacg gccgacgggc tcacgctgac gaccgtggcc ggcgccgccc
    16081 cgatgggttg gtcgaggaat cgttgcacgg cgccggtgat cgacgagacg cgccgcagcg
    16141 gggacgtcgg ctgcaggagg acgacgccgt cgaccgcgcc tctgtggctc tcgtaccagg
    16201 cgagcgcatg gcgcagcacg tcgccggacg tcgccgtgtc ggttgccagc tcggcgggcc
    16261 tcagccaggg aacgagcgca ccgacgtcgc gtccgatggt cgcgagttct tcgtcgtccg
    16321 tcgtgaccag cacgtcgcag aacgtcccgg ccgcgagcgc aacgtcgatc gaccacgcga
    16381 tcagcgggcg gccgccgagc ggccgtgcgt tcttcccggg cagtcgcttg ctgccctggc
    16441 gcgccggaat gatggccagc aggcgcggct cagccattga acagtccgga gatctcgttc
    16501 tgcgctcgct ggaaatcctc catccggccg atgtccagcc agtactcgtg cacaggaaac
    16561 atgttgacgg cactgccggc ggcgatctgg cgctcgagca acgcgggcat gtcgacgcgc
    16621 tggccgggcg ccaggccacg aagtatttcc ggggaaagga cgtagatgcc cgcgttgatg
    16681 aagtagcggt acgacggctt ctccaccatc gaggtgatgc gcatgccgtc gctttcgacg
    16741 acgccgtagg gaacccggtg ctcgtgttcg cgcacgcaca tcgtcgcgat cgcctggtga
    16801 cggtcgtgga aggccagcag cttgttggcg tcgagcgtcg tgagcaggtc accgttcatc
    16861 acgaacatcg gctcccggat ggcgtcggca ggcagcaggc tcagcgcccc gcccgtgccc
    16921 agcggctctt cctcatggac gtactggatg tcgatgtccc agcgactgcc atcgccgatg
    16981 cgctcgcgga tcatctcggg gcggtaatgc gtcgagatga agaatcggtg gaagcccgac
    17041 cgcgcgaagt tctcgatgat gatctcgaga atcggcttgt cgccgacctg cagcagtggc
    17101 ttggggcaat tgtccgtgag cggctgcagg cgcgtgccga acccgccggc catcaggaat
    17161 acggcgttgt cgcgccgcgt gccgtgcagc aggccgtgca gagtctctat gccgacgaca
    17221 cgaccctccg cgtcgaccag ggggatctgc gagatctggt ggcgctccat cagctggagc
    17281 acgctcgcct tgctcgatcc gacccgcgcg gtgcggggct gccggttcat ggcctgcgag
    17341 accggcgctt ccagcgcgac ctttcggatc aacgcgcgcc gcacgtcccc gtcggtcacg
    17401 gtgccggtga cgcggcgatc ttcgtccacg acgaccgcga tctgcagtcc gccgcggtcg
    17461 agggccgaca tggcttcctg caacgtgctg gtcgccctga tcaaggcgaa ttcccaattc
    17521 ttcatttgtc gtcctgactg gctggctacc gcaagagcgc ccatcacagc tcgatgagct
    17581 cgtcctcgcc gaaatcccgc ttcgcgacgc ggccgaccac ctcgtcccat tgcgccggtg
    17641 aaagaccggt accgggccgc ttggtgtcga gattggactc gtcgaacgtc tccccggcgc
    17701 ggatcgggcg cgcggcgacg atcgacttgc gggcgatcag cctgttgcgc agctcgcttg
    17761 cggtgggagc cttgatgccg tcgcccagcg ccacctcgat gttgcggatg gcgctcgcca
    17821 tcgccgcgaa ctggtccggg tcgatgctgg cccggtgatc cgggcccggc atggagcggt
    17881 cgagggtgaa gtgcttctcg atgatcgtcg cgccgagcgc cacggccgcg atgggcacct
    17941 cgatgccctc ggtgtgatcc gagtagccta ccgcggtgcc aaaagccgcg gacaaggtct
    18001 gcatcgcgcg caggttgacc tcgtgcatcg gcgccgggta ttcggtcgtg cagtgaagga
    18061 tggtgatgcg cgtccgcggc gtgccgttgg cctcgatgat gcccagcgcg gtctcgatct
    18121 cgccgagggt cgccatgccc gtggagagga tgaccggcaa cccctcgcgc gtcatgtgcc
    18181 gcagcagcgg aaggttggtc aattcgcccg acggcacctt gacccggtcg acgcccagcc
    18241 ggatcagcaa gtcatggctg tcgcggtcga acgccgtcga gaagaagccg atcccgcagc
    18301 gacggcactc cgcgagaagc cgacggtggt cgtccgcaga cagctccagc tttcgcacca
    18361 tttcatattg cgactcggtg gcgccggtgg cctgcttctg gtagtcggcc ttgggcgcat
    18421 gccgggcgat gctccggcct gtctggaagg tctggaactt gacgaggtcg gctcctgcct
    18481 gcgccgcgcg cctgacgagc tcgagcgcga gctcgatgtc gccgttatgg ttgacgccgg
    18541 cttcggcaat gatcaatgtc ttcatttgat tcgcttcgcg ggaatgccga catagaccgc
    18601 ctgttcgtcg caggacctga cgacgacaga gccggatccg atcacggtgt cctggccgat
    18661 gcggagcttg tcggcattgg acccctggtt gatggcggct ccggtgccga tccatgcgcg
    18721 agcgccgacg tggacgttgc cggagatgtt ggcgcccggc gagacattga cgaaatcgtc
    18781 gatccggcag tcatgcccca ccgtggcgtt gaggttgaat atcgagaatt ggccgacctg
    18841 gatgttgttg gtgaagcgga cgcccgcgca cacgatcacg ccccgctgcg cctcgacctt
    18901 cgcgcgctgg ccggcgccga acgtggccga tggatgcgcg aggctcggga acgtcagccg
    18961 atcccgatgc cgcatcgcga cgcgctctcg gacgcggttc tcgccgatgc cgacggcgaa
    19021 gggtatcgtg ccgagggagg cgagttcgga ctccaggatg gccggcgcgt cgaactgcca
    19081 cgccgccagc tcgacggcgt ccgcggcgag atagatgggg tcgtatccca gcgcttcggc
    19141 gatgtcgccg acttcgcgcg cgaacccgga ggtcccgaag atgccgagct tcgtcatgtc
    19201 tgtctccctg gccggcctgt cgacctcaaa ggtcccggaa acgcttcttc aggacatccc
    19261 cgtccggccg gaagttcctc agcgcggaaa ggatgcgtgc ggtggattcg ccgtcgccgt
    19321 agggattgac cgtgccgctg caatccatgc gcatggcctc gcagatggcg gcgtggatgt
    19381 cgtcggccgt cgcggcgcag tggatcaccg acgcggcggc cagacgaccc cgctggcgat
    19441 cgccgatgtc gacggtcgga atgccgaagg acggcgcttc gcacaggccg ctcgaggaat
    19501 tgccgacgac ggcgtcgaca tgcgacatga ggctcaggta gcgttgctgg ccgagggaag
    19561 tgaacacgtg cgccagggga cgctcggcgg cccaggcgtc cagctcgctt gccaattgcc
    19621 ggccccctgc gtccgcattc ggccgggtga accacagggt cggcgcatcc ggcagccggt
    19681 ccagcgcggc cagcaacatc tgctgctgcc ggcttgccgc gccgggctcc agagtgacgg
    19741 gatggaacgt caccaggaga ttgcgtgcgc ccagcggatg gccgagcacc tgcccgagcg
    19801 cctcgcgatc gagcaaggtc gttcgccgca ggtggtcgag gccgggattg ccgacgatcc
    19861 gcacccgcgc cggatcctcg cccatctgtt cgacgcgccg cgccgcgccg gcgttcgtga
    19921 cgaggtgcag atgcgccatc ttcgtgatgg catggcggat cgattcgtcg atcgctccct
    19981 cggtggtgtc gccgccgccg atgtgcacga gcggcacgtt gtggaccagc gccgcctcgg
    20041 cagcagcgag gatctcgaag cgatcgccca gcaccacgag gagctccggc cgcagcgcct
    20101 cgagcgcgtc ggagaagccg atcagaccca gcgccatcga tttcgcgatc gctccggccg
    20161 agtcgctggc caggagcatg tcgacacgcc gcgcgatggg caggccgtct gcctcgatct
    20221 ggcgcaccgt catgccgaac tcggtggaca ggtgcatgcc ggtgacgatg agctgaaggt
    20281 cgaactccgt ggacgcatga agctccttca gcacccagta aagcagcccg tattcggcgc
    20341 ggctgccggt gacgacggcg acgcggcgtc tcatggcctt cccggcagca cgctgctggg
    20401 cagattcacg acccgcgctt ccagccattg cgcattgtcg agcgcgccgc gcggcgcatg
    20461 cgcatacagc ggcagccggg tcatcagcgt ccagatgggg cgtgtcatga cgccggcgtc
    20521 gtttgtggcc ttcagcaggg cgtcgcgctg ggccgggccg tcacagatca cgccattgag
    20581 ccagtagttc gagcggcaat gctccggctc gacgatgggc tggagatcgg agtcctggaa
    20641 gaacgcgatg tagcgcgcgg cgagggcgcg cttcgattcg aggaacgccg gcaactgctc
    20701 cagctgcgcg cagcccagtg ccgcgttcag gttgggcagg cggtagttgt agccgacctc
    20761 gtcgtggacg aactcgtagg gatgcgcgat cttggccgtc gtcgtgaggt gcttcgcgcg
    20821 cctgccgatc tcctcgtcgg ccagcagcat gccgccgccg ccggtggtca tgatcttgtt
    20881 gccgttgaag ctcagcacgc cgatgcggcc gaagttgccc gtgtggcggc ccttgtagta
    20941 gctgcccagg gactctgcag cgtcctcgac cagcaccaga cgccagcgtt cgcacaccgc
    21001 gaccagcgcg tcgaggtcga ccggatggcc gaaggtgtgc atgggcaggc aggcacggat
    21061 gcggcggtgg ccctcgcgcg tgcggcaatc accggcgtcg tcgaccaggg catgctcctg
    21121 gagccaggcg tccagcgccg cgggcgacaa ccccagcgtg cggcggtcga cgtcgataaa
    21181 cagcggctgt gcgccgcagt acgcgatcgc gttgcacgtc gcgacgaagg tcagcggctg
    21241 cgtcacgacc aggtcgtcgc gctcgacgcc ggcgagaagc agcgcgatgt gcaacgctgc
    21301 cgtcccgttc atcacagcca ccgctcgcgg gctgccgcag aaggccgcgg tctcgcgctc
    21361 gaagcggtcg acgtaggcac ccacgctcga cacgaaggtc gactcgatcg tgtcgaggac
    21421 gaactcgcgc tcgcggccgc ggaaaaccgg tgcatgcagt gggatgaatt cccgcgtgcc
    21481 gtactgttct cggatgaaat cgaccaggcg ttcgaacatc cccgacctca gacgttgtag
    21541 atgtgtgcct tgtacctggc gaggttggcg ggccgcgtca gccagtcgat ggtgcgggcg
    21601 agacccgcgc ggatgtcgac gcgtggctcg aatcgcgtca gctcgcggat acgcgtattg
    21661 tcgccccaga gccggaacac ctccgacttg cccggacgaa tgcgatcctg gtcggtcacg
    21721 aactcgacgt cgctgcccat caattccttg atcaggtgca gcgtgtcgcc gaccgagatc
    21781 tcgtagttcg acccgatgtt gacggtctgg ccgatcgctt cgccgcagcg cgccagctcg
    21841 aggaatcccc ggcaggtatc ctcgacatag ttgaagtcgc gcgtcggcgt gatgtcgccg
    21901 agcttgatct gcctcatgcc cgacgcgatc tgcgtgatga tggtcgggat caccgcgcgc
    21961 gccgactggc gcgggccata ggtgttgaag ggccgcgcga tcgtcaacgg caggttgaat
    22021 gcgttgaaat agctcatggc catcgcgtcg gcggcgatct tcgaggcact gtagggcgac
    22081 tgcggctgca gcgggtgctt ctcgtcgatc ggtacgtatt gcgcggtgcc gtagacctcg
    22141 ctcgtggagg tgtggatgac gcgagccacg ccgttgtcca gcgcggcctg gcagatgttg
    22201 agcgttccct tcacgttcgt gtcgacatag ctgtcgggcg ccacgtacga gtatgggatc
    22261 gcgatcagcg ccgccagatg gaagacggcg tcgacgccct tggttagctt gcgacaaaaa
    22321 tgggggtcgc gcacgtcgcc gctgacgacc tcgatgtcct tcagacaatc gacatcctcc
    22381 agccaacccc acgagttgaa cgagttgtac tggctcagcg cgcggacctg caggccttcg
    22441 cgggccagca gctcaaccag gtgcgagccg atgaaaccat cggctccggt gacgaggaca
    22501 tgcttcatgc ttcttgcctt gcagggcgaa tcggtgcgcg gcgaccgcgg atcaacggcg
    22561 ttcggtcgcc agccgccgga tgcgcggggc cacgcacagg atgctggaga gtaccgcggc
    22621 cagcagcagg cccgctatgg cgaatcggga gcgcttgggc ttgctcttct tctcgggtac
    22681 cagtgcccga tcaacgacct ggatgaggct gtcatctttg ctttcgtcca ggcgagcctc
    22741 ctcgtactgc ttcgacaggg cctcgaacag cgattcctgg tacttgaact cgcggaactt
    22801 gctgacgtag tcttgcgact gcgcggagtc cgtcgactgc tgctcgatcg cgcggaggcg
    22861 tgcgcgcagc gccgatacgg cggcgtccag gtgcatcatc tcgggcgagc tgctggccat
    22921 ctgcgtgctc atggcgctgc gccggacctc gagggccgtg agctctccca tgaccttggc
    22981 gtactcctgg gtggcggcgg taggttccga ccgaagcgcg ccagggttga agccgcttgc
    23041 ctgcagcgcc gcctgggcct gggccagctt ctcccgcgtc gcctgcaact ggttctcgaa
    23101 gaagacgcgc ttgcgctgcg cctcggtcag cgccatgccg gccgtgagct ggcgcaggta
    23161 ctcgatgtac tggccagcca tggcggcggc gcgctgcgga tccgtgtcgt ccacttcgat
    23221 cgagatgagg ccgtcctttt tgccgagcgt gacacgggtg cgcgacttga gcgcgtcgcg
    23281 cgcctcgacc aggtatttga cttcgtacag ctgccgcaaa tggaagtgat cgatcatctg
    23341 gtcggcgatc gactcgctct cgagcagcga gacgtagaga tcgccggacg tcttcgtgcc
    23401 catgcccgtg gcgaggccgg cgagcggccc cagcgattgc aaggcgagtc caaggccgga
    23461 tccgccgctg gtctggggcg gcaggaaggt ggccttcgcc gtgtactgtg gggtgatcag
    23521 catcgacagc aggaacgcca gcaccccgcc cgcgagtgac gaggcgacca ccaggcgcca
    23581 tcgcctggcc acgatcagga cgagatcgag ccagttcggg tcgtcggttg tgtcgatatg
    23641 ggaggaggtc tgcatggtca atgatgtcgg gcggcggcgc agcgtctctc gcacgcttca
    23701 gccttgcgtt gcctaaagcc gccggcggcc gccgtcattt cgccgggcgc cggtcagagc
    23761 cccagcgcct tgatgccggc cagccccagg ccgaactggt agaggatctg cgtccagtcc
    23821 ttcgcgtcct ggacgaagga ggatctcgag agctcctctg gcacgaagac cgtgtcgccc
    23881 ggcagtacct ccgcagtgag gaaatcgctc gaatgccaga acgaggcgtc cgcgcgcgcg
    23941 ctgagcaccg agccgttcgc gcggatgacg aacatgctgc tcttgtcggc gtcgcgcgtc
    24001 gggccaccgg ccaggcgcag gtactgctgt acgtcgtgtc cgggcttgaa caggaagttg
    24061 ccagtgttga acacgctgcc gaagacgccc accgaggagt tgcgcgtcgg gatgttgatg
    24121 atgtcgccgt cctccagcgc gaggtcgggc agggccttgt cgtcgggctc gatctccagc
    24181 accacgcggc cggtcggctt gatctgccgc aggcgttcga gcaggcgcgc gttcgcggtc
    24241 gtcgcggcgc cttgcgtggc cagttcctcg gcgttggtgg cgcgcgtcgt cgccgcggcc
    24301 ttgctcaggt cggtctcgag gtcgcgcagc gcccggtcgt agttgagctg ctgtgcctgg
    24361 cgcacgctct cgcgccgaaa atcggtgccg tagggatacg cggccgaggt catgccgccg
    24421 gcgacgcgca gcgcgtcctc catgcggctg cctggcggca ggatgtagtc gccgggatgc
    24481 aggacctcgc cctcgatccg cacccgctcg ttctggtgca gcttcggcag cgaggccgac
    24541 acgacgctga aggcgtgcac gatctcgccg ttctgcagcg gctccgtgcg atgcgccggg
    24601 agcgcgagtt cgctgacgcg gccggtctcg cggtccgaca gcctctcgac cgccacgcgc
    24661 gtgtcgtcgg cgaccgcggt gaagccgccg gccatgtgca gcaggtcgtc gagcgtctcg
    24721 ccggacttga gctcgtagat cgcgggctgg ttgacgctgc ccagcagcgc gacctgcttg
    24781 ccgaccgggc ccacgaagac cacgtcgtcc ggctgcacga ggcggtcggc gtcgcgccgg
    24841 cccttgagca gcaggtcgta gaggtcgaac gaggcgactt cgttgccgcc gcgcatcagg
    24901 tggatgtcgc ggaagctgcc cgcggcggac ggccctcccg cccgcatcag cacgtgcagc
    24961 accgaggaca ggccgttgac ggtcaggctg ccgcggcgct gcgcgaagcc gcccacgaag
    25021 acgcggatcg gccgcacctg cccgaggttg gccgtcagtt cgaagttggt gaacacatgc
    25081 gcgacctggc ggcggatcac gttggcgaga tccgcgttgc gcacgccgcc cacggtgatg
    25141 gtgcccacgc ggggcaccga gatgccgccg gcgcggtcca ccgtcaggcg gaggttggcg
    25201 tcggccgaac cccagatgtt gagcgcaatc tcgtcacccg ggcgcacgat gtagtcgccc
    25261 ggcacgctgg gcagcggatc ctgcgtctgc agcgacgccg cggggtcggt catgagctcg
    25321 ctgccgaagc ggcgcacctt gccgtcgacc tgctgctgca cgtagcgctc gaattcgctc
    25381 ggctcgtagg gcacgctcgc cgcaagcgcg gggggctccg gctggtcgcg ccggttgccg
    25441 cggttgccgc cggcctggtt caactgcgtc gtggcgcgca tcgtgccgct gtttgcgccg
    25501 cccacgccgt tggcctgcga tccgctgccg ccggcgttgc cgaagtcgat gtcgctggca
    25561 ttgccgtccc cgggcttggc ggtggcgtcc agcgagggaa tgccgccgcc gaactgggcg
    25621 tgggcgggca cctgcagcag gacggccgcg atggatagcg cgaagccgcc gagcaacgac
    25681 aggcggcgat gcggcgaagc gaggcgtcgc acggacgatg gagtcatcgg catgaaggca
    25741 tggccgcgcg ctgccgcgag agggcgccct gcgcaggaag ttgcctgaga aatctgacga
    25801 gtcgcgcatt ctagcccgca gctcccgcgc tatcgccgtg ccacgggcgc gggcccgcct
    25861 cccgctaaaa ttcacaaggt cgtcaaaacg aacccccgct gcgtggcgcc tcgtcgcgcc
    25921 gggccgacaa tccctgaatg agcgcttcca ctcccacctt cctgcccttc gccctgcccg
    25981 agatcggcga cgaggagatc gccgaagtcg tcgacaccct gaaatccggc tgggtcacga
    26041 ccgggcccaa ggccaagcgc ttcgaggcgg cgttcaccga gttcctcggc gaccctgcgc
    26101 tcgagtcgat cgccgtcaac tcggcgactg ccggcctgca cctggcgctc gaggcgctgg
    26161 gcatcgggcc gggcgacgag gtgatcacga cgacgcacac gttcacggcg accgccgagg
    26221 tcgtgcgcta cctcggcgcg gacgtggtgc tggtggacat cgatcccgcc acgctgaaca
    26281 tcgacccggc ggcggtcgag gccgcgatca cgccgcgcac gaagtgcatc gtgcccgtgc
    26341 attacgcggg cctggcggcg gacatggggg cgatcctcgc gatcgcgagc aagcacggcc
    26401 tgcgggtcgt cgaggatgcg gcgcacgcgc tgccgacgct gtccgccggc aggctgatcg
    26461 ggacgctcgc gtccgacatc accgtcttca gcttctacgc caacaagacg atgacgacgg
    26521 gcgagggcgg catgctggtg acgcgcgatc ccgagctcgc gaagcgtgcg aaggtcatgc
    26581 gcctgcacgg catgagccgc gacgcgttcg accgcttcac cgcgacgaag ccgagctggt
    26641 attacgagat cgtcgcgccg ggcttcaagt acaacctgac cgacatcgcc gccgcgctgg
    26701 gcctccagca gctcaagcgc ctgccggcgt tccaggagcg ccgcgaacag ctcgccgcgc
    26761 gcctgaacgc gggccttgcc ggcctgccgc tggtgctgcc gccgaccgcg cccgcgggcg
    26821 accggcattc gtggcacctg tacgtcgtgc ggctttccga cgaggcaaag gtgccgcgcg
    26881 acgagttcat cgagcgcctg ttcaagctgg gcatcggcgt cagcgtgcac tacatcccgc
    26941 tgcacctgca gccgtattgg cgtgagcgct acggcctgaa gcccgagcag ttcccgcaca
    27001 gccagcgcgc ctacgagcgc atggtgagcc tgccgctgta cacgcgcatg accgacgccg
    27061 acgccgaccg cgtctgcgcc gcggtgcgcg aggccttgca ggcctgacgc cgccggcgat
    27121 gttcaagcgg gccttcgacg tcgtcgccgc gctgctcggc ctggccctgt tcggcctgcc
    27181 gatgctggcg gtcgcggcgt ggatcaagct cgactcgccg gggcctgtgt tcttccgcca
    27241 ggagcgcgtc ggccgcggcg gccggccgtt ccgcatccac aagttccgca cgatgcgcgt
    27301 cgacgccgag gcacgcggcc cgcagatcac cgtgggtgcc gacgcccgca tcacgcgcgt
    27361 gggccgcatc ctgcgcgaca accgcctcga cgagctgccg cagttcctcg acgtgctcgc
    27421 gggcgacatg agcctcgtcg gcccgcgccc cgaggtgccg cgctacgtcg ccgtctggcc
    27481 gcccgcgctg cgcgagcgcg tgctggcggt gcggcccggc atcaccgacc cggcctcgct
    27541 cgagttccgc gacgaggcct cgctgctggc cgccgcggcc gacccggagc gcgagtatgt
    27601 cgaggtgatc ctgccgcgca agctcgcgcg ggccgccgac tacgccgacc acgcttcgct
    27661 ctggaccgac ctcgccgtga tcgggcgcag cgtcggcgtg ctgttctcgc gctgagctcg
    27721 ggcgcaatac ccccataccg taccagatcc gacgtcccgt gaatccgtcc ccgatcgtcc
    27781 cgtcgcctac gttctggcat cgcctcgacc gcttcctcgc gcgcctgcgg ccgcaccgcg
    27841 agccgttgtc gctcgtcgtc gacggcctgt tggtggtgct ggcctggaac gtgacctacc
    27901 tgttccggct cggcttcgag cgctggctgg aggcgcgccc ttggtacgac ccgtgggtgt
    27961 tgctcggcat cgttttcgcc tacctgggca tgttcgcgct gctgaaggtg ccgcagggca
    28021 tgtggcgctt ctccggcttc ggcgaggtca agcgcctgac ctgggcctgc ctggtcgcgg
    28081 gggtgtgcag cgccgcggtc gtgatgggcc tgcagctgta cggcgtcccg cgttcggtgc
    28141 tagcgctgca tcccgtcgtc acgctgatgg cggtgtgcat ggtgcgcatc ggctaccgca
    28201 tggtctacga gcattcgcgc gcgcagatta ccggcggcga cggcgaggtg cgccgggcgc
    28261 tgatcctcgg tgccggcgag gccgccaagc gcctgatggc ggggatccac cagcagggct
    28321 ggatcgtcct gggcctgctg gatgacgacc cggccaagca gggcgcgcgc ttcgcgggcg
    28381 cgccggtgct cgggcccctc gaggcggtgg gcgacaaggg cgtcgtcggc gccgcgaccc
    28441 acatcgtgat cgcgctgccc ggcgcgagcg cccagcagcg tcgccgcgcc ttcgagctcg
    28501 cgggggcgac ggggcttccg gtgttgaccg tgccctcggc cgacgagctg cgcgccggca
    28561 gcggcaacat ccagcgcctg cgcgacatcg agccggaaga tctgctcggc cgtgaaccgg
    28621 tgcgcctgga cgagcgcggc atcgacaagg tgctgcacgg tcgcaccgtg ctgatcacgg
    28681 gcgcgggtgg cagcatcggc tcggagcttt gccgccaggt ggcgcgctac gaaccggcgc
    28741 ggctggtgct gttcgagctg tcggagttca acctctactc gatcgagcag gagctcggcc
    28801 agcgcttccc cgggctgccg ctcgtgcggc tgctgggcga cgtgaaggat ctcgaccagc
    28861 tgcgcgccac cttcgcgcgc acgaagcccg acatcgtctt ccacgccgcc gcgttcaagc
    28921 acgtgccgct ggtcgaggag cagaacgccg gcgctgcgct gcgcaacaat accctgggga
    28981 cgtaccacgc ggcgctggcc gcggccgaga accaggtgtc gcgtttcgtc ctcatcagca
    29041 ccgacaaggc ggtcaatccc acgaacgtga tgggcgcgac caagcgcgct gcggagatgg
    29101 tgatctcgtc gctcgtggcg cgcttccccg gcacgcgctt catggcggtg cgtttcggca
    29161 acgtgctggg ctcctcgggc agcgtgatcc cgaagttcaa ggagcagatc gcgcgcggcg
    29221 gccccgtcac ggtcacgcat ccggacatca tccgctactt catgacgatt cccgaggcgg
    29281 cgcggctggt cgtgcaggcg gcggcggtcg gcgagtcggg gcaggttctc gtgctcgaca
    29341 tgggcgagcc ggtgcgcatc gtggacctcg cccggcagct gatccgcctg tccggccatg
    29401 cggaagacag catcgcgctg gagttcaccg ggctgaggcc gggcgagaag ttgttcgagg
    29461 aattgttggc ggacgacgac accacgctgc cgacggcgat cgcccgcctg cgcctggcgc
    29521 gcctcggagc ccaggacggc caggcgctcg aggaactgct gcggttcgca cgtggcgaag
    29581 tcggggccat ggacgatgcc accgtgcgca ccgagctgag gaaggtcgtg cccgaataca
    29641 agtgagcgca tgcccgcggg agtatgcgat gtaaccctgc tcccacctct gggggtgcat
    29701 tgacaggtat ttgacaggca aaataccgcg gttgatgccc gccatcgcgg gctgcgtcgg
    29761 cgcccagtgc gctatcgggg gcctgcggcc cttcccgcac ccatgacctt tgccgccgct
    29821 ttcctcgtct cgttcctgct tgccctcgcg atcgtccggt cgcagggacg tcatgggcgc
    29881 ctgtcctatg accacgacct gtcggggccg cagaagttcc acgcggccgc cgtgccccgc
    29941 atcgggggag tcggcatctt cgccggcgtt ctggcggcga tcttcctggt ggcggcacgc
    30001 gagcccctgg acgtcgaccc gatgttcgtc ctgctcgcct gcgcgatgcc gaccttactg
    30061 tccggcctgg tcgaggactt caccaagcgc gtgccgccgc gctggcggct ggtactgacg
    30121 gcgatgtcgg cggtgctcgc tctgctgtgg ctgggcgcgc ggatcgagcg cctggacgtg
    30181 cacgcgctgc agtggctgct gctgtggccg ctggaggccg cggcgctgac ggtcttcgtc
    30241 gtcaccggca ccaccaatgc cgtcaacctc atcgacggct tcaacgggct tgcctcgatg
    30301 tcggtggcca tcatgctgtc ggcgatcggt ctcgtcgcct ggtgggtgca cgacgcgctg
    30361 gtgtgcgagc tcgccttcgc ggtcgtcggc gcggtgctgg gcttcttcgc gttcaacttc
    30421 ccgcgcggca ggatcttcct gggcgacggc ggcgcctact tcatcggctt catgctcgtc
    30481 gagctggggc tgctgctgct gcatcgcaat ccgcaggtgt cggcgatgtt cccgctgctg
    30541 gtgtgcggct acccgatctt cgagaccatc ttcacgatgt accgccggcg cttcgtgcag
    30601 cgcaccgcca ccgggctgcc ggacgcgatg cacctgcacc acctcgtgtt ccgccgcgtc
    30661 agccggcgcg cgttcgaccg ccagggccgg ccgcgttcgc tcacgtggcg caactcggcg
    30721 acctcgccct acctttggat ggtctgcgcg tgcgcggcgg tgccggccgt ctgcttctgg
    30781 aacgatacct atgtgctgct ggccttcatc ggcgtcttca tgctgggcta cgtcgcgttg
    30841 tatcggcgcc tcgatccgcg cctcgagcgg gcactggcgg ccaaggcgga cccctcgtcg
    30901 gccgagcttc gcatcgcgca tacgctgacc ggtaccgaag actgaccggc cgagctcgcc
    30961 ggctctcggt ggggtcggat cgaaagggct acacggcggt ggcgaagctg tcaatccctt
    31021 gaaaggttcg cgcagtttca cccgggtata ttcgggttag tatggacaaa atgtgtcggt
    31081 cgaatgaccg gccagcccaa ggcgagggcg ctggagagcg agcgatcggg gcctcgaccc
    31141 ggtcgggccc gcggaatgcc gggcggccct cccagcgcgc agacgaggat ctcatgtacg
    31201 caatcgacga cgtcgcggcc gcgacgcgaa cggcatcatc atcgtggcgc ccggcatcct
    31261 ccatcgacgc gcgcacgcgg cgggcggctc gatgacgccc atcggactgc atcccctgga
    31321 gcatgcgctc aagcgcatct tcgacctcgc cagcgtcgtc gtgatcctgg cgctgttcgg
    31381 ctggctgatg gtgatcatcg cgctgctcat ccgcatgaac ggacgcagcg tgatcttcgg
    31441 ccacgagcgc gtcggtcggg gggggcggac gttccagtgc tacaagttcc ggtcgatggt
    31501 gccgaattcg cagcaggtgc tgcaggaatt gctggagcgt gatccgcagg cgcgcatcga
    31561 gtgggagcgc gacttcaagc tgaaggacga tccgcgcatc acgcgcatcg gccggttcat
    31621 ccgcaagacc agcctggacg agttgccgca actgtggaac gtggtcaagg gcgagatgag
    31681 catcgtcggt ccgcgccctg tcgtccagga agagttcgat ctctactatg gccatgcccg
    31741 cgagcactat ctgagcgtcc cgccggggct gacgggcctg tggcaggtga gcgggcgcaa
    31801 cgacctcgat tacgagcagc gcgtcgagct cgacaagcgt tacgtcgaga gctggaacgt
    31861 gttttccgac tttctgatcg tgatgcgtac cgtcaaggtg atggtggcgc gccgcggcgc
    31921 ctactgacgc gcggcgccgt cggctggcgc gcttgcagag ctcgttgcgc gagcctgcgg
    31981 ccacctgcgc gaagtgcggt cgccgtgcca tataatctgt tcaatgattg aacagtcgtc
    32041 gtcgcccggg cgcgacgagc cggcccagga cgcgcactgg cgcctgctct cgctgatcga
    32101 acagcatccc gagtactcgc agcgccgcct cgccgaggcg atgggcgtga gcctgggcaa
    32161 gacgcactac ctgctgaagg cgctgttcga gaagggcctg gtcaaggcgg gcaagttcac
    32221 gcgcaacccg acgcagatga agtacgtcta cgcgctgacg ccggccggca tgaagcaccg
    32281 catgcagctg acacgcgaat tcctgcagcg caaggagcgc gagtacgtcg ccctgcgtca
    32341 cgagatcgag cggctgcgca ccgagctcgc ggtcgatggc cggaactccg agccgaatcc
    32401 ctgaacccgc cgccgcggcc acgaatcccc ggatgtccac cgtcaccctg tccaacggca
    32461 cgcgcttcga cgccgaagcc ggcgccacga tcctcgacgc cgcgctcgcc cgcggcgtgg
    32521 tgctggagca cggctgccgc acgggccgct gcggcagctg caaggcgcgc gtcgtgcacg
    32581 gcgagaccgc gccgctgcga tcccccgtct ccctcgcgga cgacgaagcc gcccgcggct
    32641 gggtgctcac gtgcacgcac gaggcgcgct ccgacgtcgc gctggacatc gaggatctcg
    32701 gcgcgctcgc cggcatcgag gcgcgcgtgc agccggcgcg catcgacgcg ctggagtcgc
    32761 tcgcgcccga tgtcgtgcgc gtcgtgctgc gcctgccgcc gcgcgcgccg ctgcgcttcc
    32821 tggccggcca gtacatcgac gtgaccggcc cgaacggcgt gcggcgcagc tattccatcg
    32881 cgagcgacgc cgccgcgggc gatcgcctcg agctgcaggt gcgccgcgtc gacggcggcg
    32941 tcctgagcgc gtactggttc gaggccgcgc gcgcgaacga cctgctgcgc ttccacgggc
    33001 cgcgcggcac cttcttcctc cggccggtcg cggagctgca cctcgtgatg ctcgcgacgg
    33061 gcaccggcat cgcgccgctg ttggcgatgc tgcgccaggt cgcggcgatg cccgagggcg
    33121 agcgcccgct gtccgtctcg ctgtactggg gcggccgcca cggcgtcgac cactacctcg
    33181 acccgcgcga cgcac
//
LOCUS       DASVLN010000932         4263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_264642, whole genome shotgun sequence.
ACCESSION   DASVLN010000932 DASVLN010000000
VERSION     DASVLN010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264642"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VF453_21890"
     CDS             <1..336
                     /locus_tag="VF453_21890"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7690374.1"
                     /translation="VALRAAGLKRRVALIVPGFQAALEVVLASDFAACVPEPFTRWST
                     SASRLRVLPMPVPTPEADVSLSWHPRLQGDLLHRWLREQVKALTAEALREPRPAGQRA
                     GSASSRATR"
     gene            complement(297..1007)
                     /locus_tag="VF453_21895"
     CDS             complement(297..1007)
                     /locus_tag="VF453_21895"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7690375.1"
                     /translation="MSSLPAESPAVDEGDAVEVAVLDAVESAAPAVSPPGARRAPRRA
                     RGQLRVDALLASAATVFAQKGFDGATMTEIAAQADSSIGSLYQFFRTKEAVAEALLSM
                     QMDALWRRFDDLAARAPALPTADLAREMVGFFAEFRAAHPSFATLVEMPRPVLPVVVG
                     VRVKVRERVQDVLARHAPQVDPARVEAMAPVVQHAMKTAIQLRGDLEEPMRAAATAEL
                     ETMLVGYLVARLDADPAR"
     gene            1071..1685
                     /locus_tag="VF453_21900"
     CDS             1071..1685
                     /locus_tag="VF453_21900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEX7690376.1"
                     /translation="MTPDATKTSLVLEPLSTALVLIDLQQGILEYAKAPYEAAAVVER
                     SARLVAAFRAAKAPIVRVKVGWSADRGDVLDAPVDAPPPTKGLPPDWLDDPQALPTLP
                     GDIPVLKRQWGAFTGTDLDLQLRRRGVRTIVLAGIATSIGVESTARFAWELGYAIVFA
                     EDACSGPDEAMHRQSFGAIFPRLGRVRTTAQVLEALGAGPAEAA"
     gene            complement(1690..1914)
                     /locus_tag="VF453_21905"
     CDS             complement(1690..1914)
                     /locus_tag="VF453_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690377.1"
                     /translation="MEQTTKVFQWQDYELQCSAKPAEDGRFVPDLVVSKRVWPTRARQ
                     IAVPRGQHLTEDTAIEAAYEQGIAWVRDWG"
     gene            2178..2372
                     /locus_tag="VF453_21910"
     CDS             2178..2372
                     /locus_tag="VF453_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690378.1"
                     /translation="MTKTLIVAALLSTFAAASFAQASAPADAASAPAKHHKMHKAHKA
                     HKAASAASNPQASADKKGGQ"
     gene            2535..2873
                     /locus_tag="VF453_21915"
     CDS             2535..2873
                     /locus_tag="VF453_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690379.1"
                     /translation="MPARRAPRQNHHVYVVQLDDRVWNEPAFRKANPDHVLGKPFVYV
                     GMTGLDPDLRFDRHKAGIQANRFVFRYGLRLLPALYAVYNPMPYEAAREMEVELAIGL
                     REAGYGVWQA"
     gene            complement(2880..3305)
                     /locus_tag="VF453_21920"
     CDS             complement(2880..3305)
                     /locus_tag="VF453_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7690380.1"
                     /translation="MDDIDRQLIALLRDDARMSVVALAAKLRLARATVQSRMARLEAD
                     GTIAGYTVRLKPAAQAHRIRAIMSVEVAGHRADVVRRALRGHPAVVSLHTTNGRWDLM
                     AELQADSLEAFDQVLRAVRAIEGIANTETSILLSTEKLS"
     gene            3446..>4263
                     /locus_tag="VF453_21925"
     CDS             3446..>4263
                     /locus_tag="VF453_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006495675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX7690381.1"
                     /translation="MPAPTATVDAARRTTAAHLVQAPAAVVMIRPHAFAPNPQTAADN
                     AFQRPDGADGPAARAEQAARARDEVDEAARRLRDEGVRVHLFDDAGADTPDSVFPNNW
                     FSTHAGGHVALFPMALPSRRRERRADVVELLKREYRVQDVIDYSGLEHDGLFLEGTGA
                     MVLDHLARVAYVARSRRADPIALERFCTHFNFEPIVFDTADARGRAVYHTNVLMAVGT
                     GFALVGFDLFASPHQAADVRARLAEAGREVIALDARQVAEFAANAQELRGRDGPL"
BASE COUNT          589 a         1601 c         1501 g          572 t
ORIGIN      
        1 gtcgcgctgc gcgccgccgg cctgaagcgg cgcgtcgcgc tgatcgtgcc cggattccag
       61 gcggcgctcg aggtggtgct ggcgtcggac ttcgccgcct gcgtgcccga gcccttcacg
      121 cgctggagca cctccgcgtc gcgcctgcgc gtgctgccga tgccggtgcc gacgccggag
      181 gccgacgtct cgctgagctg gcatccgcgg ctgcagggcg acctgctgca ccgctggctg
      241 cgcgagcagg tgaaggcgct gacggccgag gcgctgcgcg agccccggcc agcgggtcag
      301 cgcgccgggt cggcgtcgag ccgggcgacg aggtagccca ccagcatcgt ctcgagctcc
      361 gccgtcgcag cggcgcgcat cggctcctcg agatcgccgc gcagctggat cgcggtcttc
      421 atcgcatgct gcaccacggg cgccatcgcc tcgacccgcg ccggatcgac ctgcggggcg
      481 tggcgcgcga ggacgtcctg cacgcgctcg cgcaccttga cgcgcacgcc cacgacgacc
      541 ggcagcaccg ggcgcggcat ctccacgagc gtcgcgaacg acgggtgggc ggcgcggaat
      601 tccgcgaaga agcccaccat ctcgcgggcg aggtccgccg tcggcagcgc cggcgcgcgc
      661 gcggcgaggt cgtcgaagcg ccgccacagc gcgtccatct gcatggacag cagcgcctcg
      721 gcgaccgcct ccttggtgcg gaagaactgg tacagggaac cgatggacga gtcggcctgg
      781 gccgcgatct cggtcatcgt ggcgccgtcg aagcccttct gcgcgaagac ggtggccgcg
      841 gaggccagca gcgcgtcgac ccgcagctgc ccgcgcgccc ggcgcggcgc ccggcgcgcg
      901 cccggaggcg acaccgcggg agcggcggat tcgacggcgt cgaggacggc gacctcgacg
      961 gcatcgccct cgtcgacggc gggggattcg gcgggcaggc tggacatttg tgaggctatg
     1021 ctcataatta aatacgtgag gaattcctcg cgtagataaa ggaacgaacc gtgacccccg
     1081 acgctaccaa aacttcgctc gtgctggagc ccttgtcgac ggcgctggtg ctgatcgacc
     1141 tccagcaggg catcctggaa tacgccaagg cgccgtacga ggcagccgcg gtcgtcgagc
     1201 gcagcgcccg gctggtcgcg gcgttcaggg cggcgaaagc gccgatcgtg cgggtgaagg
     1261 tgggttggtc ggcggaccgc ggcgacgtcc tggacgcccc ggtcgatgcg cccccgccga
     1321 cgaaaggcct gccgcccgac tggctggacg acccgcaggc actgccgacg ctgccgggcg
     1381 acatccccgt gctgaagcgc cagtggggcg ccttcaccgg caccgacctc gacctgcagc
     1441 tgcgccgccg cggcgtgcgc accatcgtgc tggcggggat cgcgacctcg atcggcgtgg
     1501 agtcgaccgc gcgcttcgcg tgggagctgg gctatgcgat cgtgttcgcg gaggacgcgt
     1561 gcagcgggcc cgacgaggcg atgcaccggc agtccttcgg cgcgatcttt ccccgcctcg
     1621 gccgcgtgcg cacgacggcg caggtgctgg aggcgctcgg cgccggcccg gcggaggccg
     1681 cgtagcgggt cagccccagt cgcgcaccca ggcgatgccc tgctcgtagg ccgcctcgat
     1741 ggccgtgtcc tcggtcaggt gctggccgcg cgggacggcg atctggcgcg cgcgcgtcgg
     1801 ccacacccgc ttggacacca cgaggtccgg cacgaagcgc ccgtcctcgg cgggcttcgc
     1861 gctgcattgc agctcgtagt cctgccactg gaagaccttg gtcgtctgtt ccatcgcccc
     1921 tccggctgtt cggtgctcca gtatgcaccc gccgcaatga cgcgcacttg actcggcggc
     1981 cccgcggccc cgggcagacg tgcgaaacgg ccgccgacgc gggccggcag ctcgaccgtg
     2041 cggccggacg cagatttcgc gtggaaactg tcaaccggcc gggcatgggc gccgttgtgt
     2101 ctccaagccc gggctccccg ggcccgctcg agagagtcgg agcacaaacg aatctcgctt
     2161 gacatcggaa gtttcacatg accaagaccc tcatcgttgc cgccctgctc tccaccttcg
     2221 cggccgcctc gttcgcccag gcctcggctc ccgccgacgc cgcctcggcc ccggccaagc
     2281 accacaagat gcacaaggcg cacaaggccc acaaggcggc ttcggcggct tccaacccgc
     2341 aagcttccgc cgacaagaag ggcggccagt aattccaggg ccggcccgcg cggccggccg
     2401 tcggaatcgc gcccgggccc gtcccgggcg cagccacgtg aagggtcgac gcgagtcggc
     2461 ccttttcgtt ttccgcttcc ggctttccgg cccgcgcgcc gggacgcggg cgggggcgag
     2521 aatcgaggcc cgccatgcca gcccgccgcg cgccccgcca gaaccatcac gtgtacgtgg
     2581 tgcagctcga cgaccgcgtc tggaacgagc cggcgttccg caaggccaat ccggatcacg
     2641 tgctgggcaa gcccttcgtc tacgtcggga tgacgggcct cgaccccgac ctgcgcttcg
     2701 accgccacaa ggcgggcatc caggccaacc gcttcgtctt caggtacggc ctgcgactgc
     2761 tgccggcgct ctacgccgtc tacaacccga tgccctacga ggccgcgcgc gagatggagg
     2821 tcgagctggc gatcggcctg cgcgaggcgg gctacggcgt ctggcaggcc tgacgccgct
     2881 caggacagct tctcggtcga cagcaggatg ctcgtctcgg tgttggcgat gccctcgatc
     2941 gcgcgcaccg cgcgcagcac ctggtcgaag gcctcgagcg aatcggcctg cagttcggcc
     3001 atcaggtccc agcggccgtt ggtcgtgtgc agggagacca ccgccggatg cccgcgcagc
     3061 gcgcgccgca cgacgtcggc gcgatggccg gccacctcca cgctcatgat ggcccggatg
     3121 cggtgcgcct gcgccgcggg cttcaggcgc accgtgtagc cggcgatggt gccgtccgcc
     3181 tcgaggcggg ccatccggct ctgcaccgtc gcgcgcgcga gccgcaactt cgccgccagg
     3241 gccacgacgg acatccgcgc gtcgtcgcgc agcagggcga tgagctggcg gtcgatgtcg
     3301 tccatgcggg cggccgggat gcgcaaactg cgcgtcgtca atgagcagga tgcgcagtct
     3361 acgctgcaag ttgccagcct ggacggttcc ctccggcgcg gccggctcga agaatgggcg
     3421 ccaccgcact tccaggagac gcccgatgcc cgccccgacc gccaccgtcg atgccgcgcg
     3481 acgaacgacc gccgcccacc tcgtccaggc cccggccgcc gtggtcatga tccggccgca
     3541 cgccttcgcg ccgaatccgc agacggcggc cgacaacgcc ttccagcgcc cggacggcgc
     3601 cgacggcccc gccgcccgcg ccgagcaggc cgcccgcgcg cgggacgagg tagacgaggc
     3661 cgcccgccgg ctccgcgacg agggcgtgcg cgtccacctg ttcgacgacg cgggcgccga
     3721 cacccccgac tcggtcttcc cgaacaactg gttctccacc cacgccggcg ggcacgtcgc
     3781 cctgttcccg atggcgctgc ccagccggcg ccgcgagcgc cgcgccgacg tcgtcgagct
     3841 gctcaagcgc gaataccgcg tgcaggacgt gatcgactac agcggcctgg agcacgacgg
     3901 gctgttcctc gagggcaccg gcgcgatggt gctcgaccac ctcgcccgcg tcgcctacgt
     3961 cgcgcggtcg cggcgcgccg acccgatcgc gctggagcgc ttctgcaccc acttcaactt
     4021 cgagccgatc gtcttcgaca ccgccgacgc ccgcggccgg gcggtgtacc acacgaacgt
     4081 gctgatggcc gtgggcacgg gcttcgcgct cgtcggcttc gacctgttcg ccagcccgca
     4141 ccaggccgcc gacgtccgcg cccgcctggc cgaggccggc cgcgaggtga tcgcgctcga
     4201 cgcgcgccag gtcgccgagt tcgccgccaa cgcgcaggag ctgcgcggac gcgacgggcc
     4261 gct
//
LOCUS       DASVLN010000933         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_371899, whole genome shotgun sequence.
ACCESSION   DASVLN010000933 DASVLN010000000
VERSION     DASVLN010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371899"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VF453_21930"
     CDS             complement(<1..183)
                     /locus_tag="VF453_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690382.1"
                     /translation="MDGCALEDGCALVDGCGRTGVCVLAEGFVSTAVFVPAGGWGIAL
                     AGGSCIALICRMAASRS"
     gene            200..1528
                     /locus_tag="VF453_21935"
     CDS             200..1528
                     /locus_tag="VF453_21935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690383.1"
                     /translation="MQPGHAPGVSATAPAPVAPLHVAAPLGALSPTDVNPFAMTGSLL
                     GKMQRLGATSMEDAVRMTMQSMRNVRGVNISNAHFEQAVQGTLNALRDNGITTMRGFV
                     KEVVGPLRRHALGLSVRQGLMAGTGFNIGAILWDAKLAAPLQEALGQHFPHLSPIQLG
                     AATTAIIAASLYVADVATSYAAGKAHAGAYPTKPGGKNVPASLQNPNPISARMMAAKL
                     AVGADLTYGAREITVRGLGSIGLAAEGKPAAVQADWETGTDGPMGLAAAAGLRVFLNK
                     TDDNAGRSGVQVPMFERGAPGSRTADGGAGIDRFLQQQQRPTLQKVGDAAMSTLKAAG
                     SFVAHVPSGVGYALTSLTGATSATFLVGGLTALNVAANTAKQSMLDRGHSPDSAEAAR
                     QTVRMFGLMAVYAMWGGPMGAAAVAEERDARLSGPVPALEEMAKEAFGQA"
BASE COUNT          238 a          569 c          554 g          178 t
ORIGIN      
        1 cgatcgcgat gcggccatgc ggcagatcag ggcgatgcag ctgccgcccg ccagcgcgat
       61 gccccagccg cctgcgggca cgaagaccgc cgtggaaacg aagccctccg cgagcacgca
      121 gacgcccgtc cgtccgcagc cgtccacgag cgcgcagccg tcttcgagcg cgcagccgtc
      181 cacgaccgcg cagccgacgg tgcaacccgg gcacgcaccg ggcgtcagcg ccaccgcgcc
      241 tgcccccgtc gcgccgctgc acgtcgcggc gccgctcggt gccctcagcc cgacggacgt
      301 gaacccgttc gcgatgaccg ggtcgctgct cgggaagatg cagcggctcg gcgcgaccag
      361 catggaggat gccgtgcgca tgaccatgca gtcgatgcgc aacgtccgcg gcgtcaacat
      421 cagcaacgcc catttcgagc aggcggtgca gggcacgctg aacgcgctgc gcgacaatgg
      481 catcaccacg atgcggggct tcgtcaagga ggtggtcgga ccgctgcgcc ggcacgcgct
      541 gggcctgagc gtccgccaag gcctcatggc cggcaccggg ttcaacatcg gcgcgatcct
      601 gtgggacgcg aaactggcgg ccccgttgca ggaggcgctc ggccagcact ttccgcacct
      661 ctcgccgatc cagctggggg ccgcgaccac cgccatcatc gcggcgtcgc tctacgtggc
      721 cgacgtcgcc acgagctacg cggccggcaa ggcgcacgcg ggggcctatc cgacgaagcc
      781 cggcggcaag aacgtgccag ccagcctgca gaaccccaat cccatctccg cccgcatgat
      841 ggcggccaag ctggccgtcg gggccgacct gacctacggc gcccgcgaaa tcaccgtccg
      901 cggtctcggc tcgatcggcc tggccgcgga gggcaagccg gcggccgtgc aggcggactg
      961 ggaaaccggc accgacgggc cgatgggact ggctgccgcc gccggcctgc gcgtcttcct
     1021 caacaagacg gacgacaacg cgggtcgcag tggcgtccag gtgcccatgt tcgagcgggg
     1081 cgcgccggga agccgcaccg cggacggcgg cgcgggcatc gaccggttcc tgcagcagca
     1141 gcagcggccc acgctgcaga aggtcggcga cgcggcgatg tcgaccctga aggcggccgg
     1201 ctccttcgtc gcccacgtgc cgtcgggtgt cggctacgcc ctcaccagcc tgacgggcgc
     1261 gacctcggcg acgttcctcg tcggcgggct gaccgcgctc aacgtggcgg cgaacacggc
     1321 gaagcagtcc atgctggacc gcggccacag ccccgacagc gccgaggccg cccggcagac
     1381 cgtccggatg ttcggcctga tggccgtcta cgcgatgtgg ggcgggccga tgggcgccgc
     1441 cgcggtggcg gaggagcgcg acgcgcggct cagcggtccg gtgccggcgc tggaggagat
     1501 ggccaaggaa gcgttcggac aggcctgaca cgcggccgc
//
LOCUS       DASVLN010000934         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_103741, whole genome shotgun sequence.
ACCESSION   DASVLN010000934 DASVLN010000000
VERSION     DASVLN010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103741"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VF453_21940"
     CDS             complement(<1..1300)
                     /locus_tag="VF453_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX7690384.1"
                     /translation="MPPDQHAFQRDQPDVAAALRGELADWRQWLGRVVVLGHAALAGL
                     AVVAFNLLCDYASRGFSTVRGLHPLAPLLWTPVVTAGIAWLTRYHFPGASGSGIPQVM
                     AALQSGGPRRRLLVSLQLTVAKIVLTAGSLLGGLAVGREGPSVQIAAGVMQHARRWLP
                     RHRGLSMHGLLVAGGAAGIAATFNAPLAGVMFAIEELASRLEQRSSGLLVAAIVLAGL
                     VGVSFFGNLTYFGVIHAPAVPMRSAGWALLVALAAGALGGVFARLLHGSITGSHVGIA
                     RWRARRPIAFAALCGLAAAAVGCASQGAGFGSGYEYTRALLTGHVDTPLLYVTLRIVA
                     TWLAVWSGVPGGIFAPCLAIGAGVGHDVALLAGADVDSAALIALGMAAFLAAVTQAPM
                     TAFIIVMEMVDGHAMVLSLMAAALVASLVSRSISRPLYGML"
     gene            complement(1406..>2048)
                     /locus_tag="VF453_21945"
     CDS             complement(1406..>2048)
                     /locus_tag="VF453_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690385.1"
                     /translation="VPRPAVLPPRAPAVYVECDLPGRSPVARVDHHHPGDAGYEMPPG
                     RYLEGSSLGQVLRLLEREPSPTQRLLAAGDHCLTAAYQGACPGVDPGDLLFLRAAWQA
                     KVGGRTLSDVVTGITEAAARVRGRHDCATGEARFLDPTRLPSDLPEGAAYAGLPVRYR
                     MLLPEGVLKEMLKGGTPAAVETFIAEHEAAGRPAYGNPYRGYAGAYWEDRGAR"
BASE COUNT          311 a          765 c          760 g          212 t
ORIGIN      
        1 tgagcatccc gtagagcggc cgcgagatcg agcgcgacac gaggctcgcc accagcgccg
       61 cggccatgag gctcagcacc atcgcgtggc cgtcgaccat ttccatcacg atgatgaagg
      121 cggtcatcgg cgcctgcgtc acggcggcga ggaaggccgc catgcccagc gcgatcagcg
      181 cggcggagtc gacgtccgcg ccggccagca gcgccacgtc atggccgacg cccgcgccga
      241 tggccaggca aggcgcgaag atgccgcccg gcaccccgga ccacacggcc agccaggtcg
      301 cgacgatgcg cagcgtcaca tagagcaggg gggtatcgac atggccggtc agcagggcgc
      361 gcgtgtactc gtagccgctg ccgaagcccg cgccctggct cgcgcaaccg acggccgccg
      421 ccgcgaggcc gcacagcgcg gcgaacgcga tcgggcgacg cgcccgccag cgcgcgatgc
      481 cgacgtggct gcccgtgatc gagccgtgca gcaagcgcgc gaagacgccg ccgagcgccc
      541 cggccgccag cgcgacgagc agcgcccacc cggccgaccg catcggcacc gcgggggcgt
      601 ggatcacgcc gaagtaggtg aggttgccga agaagctcac gcccaccagg cccgcgagca
      661 cgatggccgc gaccagcagg ccgctgctgc gctgctccag gcggctcgcg agctcctcga
      721 tggcgaacat gacgccggcc agcggtgcgt tgaaggtggc ggcgatgccg gcggcgccgc
      781 cggcgacgag cagcccgtgc atgctcaggc cgcgatggcg cggcagccag cggcgcgcgt
      841 gctgcatcac gccggcggcg atctgcaccg acgggccttc gcgaccgacc gccagtccgc
      901 ccagcaggct gccggcggtc agcacgatct tggcgaccgt caactgcagc gagacgagga
      961 gccgccggcg tggcccgccg ctctgcaacg cggccatcac ctgcggaatg cccgaaccgg
     1021 atgcgcccgg aaagtggtac cgcgtcagcc aggcgatgcc cgcggtgacc acgggcgtcc
     1081 acagcagcgg cgcgagcggg tgcagcccgc gcacggtcga gaacccgcgg ctcgcgtagt
     1141 cgcacagcag gttgaacgcg acgaccgcca ggccggccag cgccgcgtgc ccgagcacga
     1201 cgacccggcc gagccactgg cgccagtcgg cgagctcgcc gcgcagcgca gcggcgacgt
     1261 ccggttgatc gcgctggaag gcgtgctggt ccggtggcat ggcaaaactg ctattgtaca
     1321 aatatatgcg cgccgcgcgg ccgcaggggc ttgcggccgg aactcggcac gagacgcgcc
     1381 ccctcgcggc gacctgtccg gcgcgtcagc gcgcgccccg atcctcccag tacgcaccgg
     1441 cgtaaccgcg atacgggttg ccgtatgcgg gccggcccgc ggcctcgtgc tcggcgatga
     1501 acgtctcgac cgccgcgggc gtgccgccct tgagcatctc cttgagcacg ccctcgggca
     1561 gcagcatccg gtagcgcacc ggcaggccgg cgtaggcggc gccttcgggc aggtcggacg
     1621 gcaaccgggt cggatcgagg aagcgcgcct cgcccgtggc gcagtcgtgg cggccacgca
     1681 cccgcgcggc ggcctcggtg atgccggtga cgacgtccga cagcgtgcgc ccgccgacct
     1741 tggcctgcca cgccgcgcgc aggaacagca ggtcgcccgg atccacgccc gggcacgcgc
     1801 cctggtaggc cgcggtgagg cagtggtcgc cggccgcgag caggcgctgc gtcggcgacg
     1861 gctcgcgttc cagcaggcgc agcacctggc ccagcgagga gccttccagg tagcgcccgg
     1921 gcggcatctc gtagccggcg tcgccgggat ggtggtggtc gacgcgcgcg accggcgacc
     1981 ggccgggcag gtcgcactcg acgtagaccg cgggcgcccg cggcggcagc accgcaggcc
     2041 gcggcacg
//
LOCUS       DASVLN010000935         9970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_371973, whole genome shotgun sequence.
ACCESSION   DASVLN010000935 DASVLN010000000
VERSION     DASVLN010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371973"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VF453_21950"
     CDS             <1..1048
                     /locus_tag="VF453_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7690386.1"
                     /translation="AGNRFSLGLGASWEPDIWGKLAGSVDAATATAQASEADLAAALL
                     SAQATFVTDYLSLREADAEIAILEATITGYQRSLQITRNRYAAAIAQKSDVLQARTTL
                     ANAQADLAALRQQRAQFLHAMAALAGQTPAAFRLPTGDWKSTAVPAIPLGVPSELLQR
                     RPDIAGAERRVAAANAQVGVARAAYFPDLTLSASGQQGASRLGELFKASSFAWSLGAS
                     LAETIFDAGARGARVDEAKASWSQAVAQYRQTVLSAFSEVEDQLSSARSLEEQQALRR
                     TASEAADQTEQEMQNRYAQGLVAYTDVVTAQASALSARRALLQVSLGRQTGAVSMISA
                     LGGGWSAKQVDDAR"
     gene            1176..1823
                     /locus_tag="VF453_21955"
     CDS             1176..1823
                     /locus_tag="VF453_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7690387.1"
                     /translation="MFDTATLASFVAVVMGLFLIPGPAVFLVLTRTAQGGRRAGIATG
                     LGIAAGDFVHTAFAAFGLSALLMTSALAFNVVKLVGAAYLVWMGVRALRTRPAPPRAA
                     SAPDLPPLPAVTPLRAFLQAIPAELLNPKAALFFLAFMPQFVRPEHGSTFAQFAVLGL
                     IFVVMSAAYSSLLAIAAVPLGRLARRLRWLARWQNQIVGSIFLGLGVKVAIQQQR"
     gene            complement(1827..3344)
                     /gene="malQ"
                     /locus_tag="VF453_21960"
     CDS             complement(1827..3344)
                     /gene="malQ"
                     /locus_tag="VF453_21960"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEX7690388.1"
                     /translation="MDLHQRCAGVLLHATSLPGPHGIGDFGPAAFRFVDWLVAAGQRV
                     WQLLPTAPIGPANSPYQSVSAFAGSPLMVALEPLVAKGWLDAPQLPEGGFDPMRVDYG
                     RVVPWRLAQLRAAATGFFARADALDRAAFDKWCTSEASWLDDYALFMALETANEGRPW
                     WTWADGLRRRDPEALAQARREHAMERTFWQFVQWCFDVQLKSIKTYANGRGVSLMGDL
                     PIFVAHHSADVWARPDLYTLGDDFQPTVVAGCPPDDMAVDGQRWGNPLYRWDRMAAED
                     FAWWTARVCRALEQADVFRIDHFRGFAGYYEIPASCPTAREGRWLAGPGKALFDAIER
                     ALGPLPIVAEDLGFITPDVVELRDACGFPGMRILQFGFGGDATHEFLPHNWIANTIAY
                     TGTHDNDTARGWWDHAGPRERAFAGSYLVAGDRDIHWAMIRACCNSVANIAIFPLQDV
                     LGLGSEHRMNTPGTVGDHNWSWRFDWPMLGAEPTRVLGLITAASGRGPFELLRLP"
     gene            complement(3361..5475)
                     /gene="glgX"
                     /locus_tag="VF453_21965"
     CDS             complement(3361..5475)
                     /gene="glgX"
                     /locus_tag="VF453_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX7690389.1"
                     /translation="MLQLPATVLPGRCEPMGAHARDGGVNVAVFSQHAQRIEFCVFDA
                     AGARELRRYELRGPHDGVFHGFLPGLGEGQVYGLRAHGPYEPACGHRFNANKLLLDPC
                     AREIVGRFDWRAEHHGYELGHPEGPRSFDVRDNAASALKARVAPPARAARGWLNAPRV
                     APEDVVLYELHVKGFGKLQPGIPPELRGTYAGLAHPAAIAHFRSLGVTTLSLLPVQYH
                     VDEPMLAGKGLTNYWGYNTLGFFCPDPRFSLTSGDPAAVNAEFREMVATLHEHGLEVV
                     LDVVYNHTPEGNEHGPTISFRGLDNASWYRLDADDRGRFENVTGCGNTVNVAHPRVTQ
                     FVLDSLRYWVEEMGVDGFRFDLAPVLGRTARGFDPDAAFFTALRQDPVLARVHLIAEP
                     WDAAPDGYQVGRFPGRFLEWNDRFRDSVRRWWLGDAQPERVGRGELARRFTASSDLFD
                     HGQRTPLASVNYLAVHDGYTLADLTSYARKHNEANGEGNRDGRDGEPCANFGAEGPTR
                     DERILELRGRVRRAMMATLALAQGTPMLCAGDEMGNGQQGNNNAYCHDAPIAWIDWPA
                     ADATMLAFTTRALALRRAHAHLRVGEWRAGAGGAPRLAWLNERGGEMDAADWHRDEVR
                     AFGCRIDPVAAPDAPPRPALLVLFNAESRPREFPLPAGRWTLALDSSLELEGAAGGPP
                     LACADSLHLPARALVVLAQAAA"
     gene            complement(5512..7689)
                     /gene="glgB"
                     /locus_tag="VF453_21970"
     CDS             complement(5512..7689)
                     /gene="glgB"
                     /locus_tag="VF453_21970"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX7690390.1"
                     /translation="MLTDDDLGALVRARHPDPFAILGVRADARGQWWVRAFLPNAEDV
                     ELVDAAGERVVVLDRRHPAGVWESPLVAPVEDYRLRVRWDSGVRDDYADPYAFGPLLD
                     EAALRAFSEGRHLRPCELLGAHPTALGEGERAVAGVRFAVWAPRARRVSVVGDFNNWD
                     GRRHLMRRRDPSGVWELFVPHMIAGDRYKYELVGPDDALQPLKADPYAFAAQLRPDTA
                     SIVAPLPERRALPSWRARANDRHAPISVYEVHPGSWRRADGRFKTWDELAEELPAYAS
                     DLGFTHVELMPITEFPFDGSWGYQTLGLYAPTARFGDPRAFQRFVDACHARGIGVLLD
                     WVPAHFPIDAHGLSRFDGTALYEYEDPREGFHRDWNTLIYDFGRPEVRCFLAGSALYW
                     IERFGVDGLRVDAVASMIYRDYSRSSGEWLPNRYGGRENLEAIELLKHINEVLVAEAP
                     GAITLAEESTTFPRVSHPTYAGGLGFHFKWNMGWMHDTLLYMRQPHVQRRLHHDKLTF
                     GLVYAFDENFILPLSHDEVVHGKGSLIARMPGDDGQRFANLRAYFAFMWAHPGKKLLF
                     MGQEFAQDTEWDYDTELPWHLLGQARNAGVQRLVRDLNRVYREERALHRQDCDAFGFE
                     WIASTEATNSLLAWIRRDTDGDAMIAVCNMTPVPQFSYVLGVPEGPTGWREVLNTDSA
                     HYGGSNVGNASAVLPVEAVASHGRSQSIRMVVPPLATVYLLPA"
     gene            complement(7740..>9970)
                     /locus_tag="VF453_21975"
     CDS             complement(7740..>9970)
                     /locus_tag="VF453_21975"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX7690391.1"
                     /translation="YLSMEFLMGRALGNALAVLGLAPDLQSALRAHPGLPAIGEVLEH
                     EPDAALGNGGLGRLAACFLDAFATLGLPSFGYGLRYTHGMFAQAIREGRQVELPDEWL
                     KDGNPWEFPRPELRHLVGFGGVVESSGGHRSWKPAEEVAAQAVDFVVPGHATQRVSTL
                     RQWHAQAARPIDFATFCRGEHLAAGQHLEAADSLNWVLYPDDSTAAGRLLRLKQEFFL
                     VSASVQDMLARHLREHGDVRSFGRHNAVHLNDTHPALAPAELMRLLLDVHGLAWEDAW
                     AITTEATSYTNHTLMPEALETWPVTMFEQLLPRHLEIVYEINRRFLDEVGERFPGDGG
                     LIARLSLIDEVGERRVRMASLAIVASRKVNGVSALHSRLMVETIFADFARMFPQRFLN
                     VTNGVTPRRWLMQANPALSTLLDERIGTGWRSDLDELAALAPQAADKALGQAFLATKR
                     ANKERLAVRIKREFGVVIDPASLFDVQIKRIHEYKRQLLNVLHVVARYQAIVADPGAD
                     WTPRTVIFAGKAASAYHAAKLIIQLIHDVARVVNADPRVGDKLKVVFLPNYSVSLAEA
                     IIPAADLSEQISTAGTEASGTGNMKFALNGALTIGTWDGANIEMAEAIGTENMFVFGL
                     RADAVAKMKSWGYDPRLYAEENQALQQVLDAIGGGVFSWGEPDRYKSLVQPLLHRDPY
                     MLLADFADYLHAQSLVDQLYAQPAAWAERALRNVAGMGAFSADRTIREYVQHVWSAPE
                     GR"
BASE COUNT         1401 a         3510 c         3694 g         1365 t
ORIGIN      
        1 ggccggcaac cgcttctcgc tgggcctggg cgcgagctgg gagcccgaca tctggggcaa
       61 gctcgccggc agcgtcgacg ccgcgacggc gaccgcgcag gcgagcgagg ccgacctggc
      121 cgccgcgctg ctgtccgccc aggcgacctt cgtcaccgac tacctgtcgc tgcgcgaggc
      181 cgatgccgag atcgcgatcc tcgaggccac gatcacgggc taccagcgct cgctgcagat
      241 cacgcgcaac cgctacgccg cggccatcgc gcagaagagc gacgtgctgc aggcacggac
      301 gacgctcgcc aacgcccagg ccgacctcgc cgcgctgcgc cagcagcggg cgcagttcct
      361 ccacgcgatg gccgcgcttg ccgggcagac gcccgcggcc ttccgcctgc ccacggggga
      421 ctggaagtcg accgcggtgc ccgcgatacc cctgggcgta ccgtccgagc tgctgcagcg
      481 tcgacccgac atcgctggcg ccgagcgccg cgtcgccgcc gccaacgcgc aggtgggcgt
      541 cgcgcgcgcc gcgtacttcc ccgacctcac gctgagcgcc agcggccagc agggcgcgtc
      601 gcgcctggga gagctgttca aggcgtcatc cttcgcctgg tcgctgggtg cgtcgctggc
      661 cgagacgatc ttcgacgccg gcgcccgcgg cgcgcgggtc gacgaggcga aggcgtcgtg
      721 gagccaggcg gttgcgcagt accggcagac cgtgctgtcg gcgttctcgg aggtcgagga
      781 ccagctgtcg tccgcgcgca gcctcgagga gcagcaggcc ctgcgacgca ccgcctccga
      841 ggccgccgac cagaccgagc aggagatgca gaaccgctac gcccagggcc tggtcgccta
      901 caccgacgtc gtgacggcac aggcctcggc gctgagcgcg cgccgcgcgc tgctccaggt
      961 gtcgcttggc cgacagacgg gcgcggtctc gatgatcagc gcgctgggcg gcggctggag
     1021 cgccaagcag gtcgacgacg cgcgctgacg cgccgtcgag cccctgcgcg cgacagggcc
     1081 gccacggcgg tgcgccggcg tgccgcgcgc gtcccgtgct tcaatgcgcg agcggccgcg
     1141 catcctgcgg cctgcagccc gaggagacgc cgttcatgtt cgacaccgcc accctggcca
     1201 gcttcgtcgc ggtcgtgatg ggcctgttcc tgatccccgg cccggccgtc ttcctggtgc
     1261 tgacgcgcac cgcgcaaggc ggccgccgcg ccggcatcgc gaccggcctg ggcatcgcgg
     1321 ccggcgactt cgtgcacacc gcgttcgcgg ccttcgggct gtcggcgctg ctgatgacct
     1381 ccgcgctcgc gttcaacgtc gtcaagctgg tgggcgccgc ctacctggtg tggatgggcg
     1441 tgcgcgcgct gcgcacgcgg cccgcgcccc ctcgcgcggc gtccgcgccg gacctcccgc
     1501 cactgcccgc cgtgacgccg ctgcgcgcct tcctgcaggc catcccggca gaactgctca
     1561 atccgaaggc cgccctcttc ttcctcgcgt tcatgccgca gttcgtgcgg ccggagcacg
     1621 gctcgacctt cgcccagttc gccgtgctgg gcctgatctt cgtcgtgatg agcgccgcgt
     1681 attcgtccct gctcgcgatc gccgcggtcc cgctgggccg cctcgcgcgc cgcctgcgct
     1741 ggctcgcgcg atggcagaac cagatcgtcg gctcgatctt cctcgggctc ggcgtgaagg
     1801 tggcgatcca gcagcagcgc tgaagctcag ggcagccgca gcagctcgaa cggcccgcgt
     1861 ccgctcgcgg cggtgatgag gcccagcacc cgcgtcggct ccgcgcccag catcggccag
     1921 tcgaagcgcc aggaccagtt gtggtcgccg acggtgccgg gcgtgttcat ccggtgctcg
     1981 ctgccgaggc ccagcacgtc ctgcagcggg aagatcgcga tgttggccac cgagttgcag
     2041 caggcgcgga tcatcgccca gtggatgtcg cggtcgccgg ccacgaggta gctgcccgcg
     2101 aaggcgcgct cgcgcgggcc cgcgtggtcc caccagccgc gcgcggtgtc gttgtcgtgc
     2161 gtgccggtgt aggcgatcgt gttggcgatc cagttgtgcg gcaggaactc gtgcgtcgca
     2221 tcgccgccga agccgaactg caggatccgc atgcccggga agccgcaggc gtcgcgcagc
     2281 tcgacgacgt cgggcgtgat gaagccgagg tcctccgcga cgatgggcag cgggccgagc
     2341 gcgcgctcga tcgcgtcgaa cagcgccttg ccggggcccg ccagccagcg gccctcgcgc
     2401 gcggtcgggc agctcgcggg gatctcgtag tagccggcaa acccgcggaa atggtcgatg
     2461 cgaaagacgt cggcctgctc cagcgcgcgg cagacgcggg ccgtccacca cgcgaagtcc
     2521 tcggcggcca tgcggtccca gcggtaaagc gggttgcccc agcgctggcc gtcgacggcc
     2581 atgtcgtcgg gcgggcagcc ggcgacgacg gtgggctgga agtcgtcgcc aagcgtgtag
     2641 aggtcgggcc gcgcccagac gtcggcgctg tggtgggcga cgaagatcgg caggtcgccc
     2701 atcagggaga cgccgcgccc gttggcgtag gtcttgatcg acttgagctg gacgtcgaag
     2761 caccactgga cgaactgcca gaacgtgcgc tccatcgcat gctcgcggcg cgcctgcgcg
     2821 agcgcttcgg gatcgcggcg gcgcagcccg tcggcccacg tccaccacgg gcggccctcg
     2881 ttggccgtct caagcgccat gaacagcgcg tagtcgtcca gccacgaggc ctcggaggtg
     2941 caccacttgt cgaacgcggc ccgatccagc gcatccgcgc gcgcgaagaa gcccgtggcc
     3001 gcggcgcgca gctgcgccag ccgccatggc acgacgcgcc cgtagtcgac gcgcatcggg
     3061 tcgaagccgc cctcgggcag ctgcggcgcg tcgagccagc ccttcgcgac gaggggctcg
     3121 agggcgacca tcagcggact gcccgcgaac gcggagacgc tctggtacgg cgaattggcc
     3181 gggccgatcg gcgcggtggg cagcagctgc cacacgcgct ggccggccgc gacgagccag
     3241 tcgacgaacc ggaaggcggc ggggccgaag tcgccgatgc catgcggccc gggcagcgag
     3301 gtggcgtgca gcagcacgcc ggcgcaacgt tggtggagat ccatgtcggg agtccgttgg
     3361 tcaggccgcg gcctgggcga gcaccaccag cgcgcgcgcc ggcaggtgca gtgaatcggc
     3421 gcacgcgagc ggcgggccgc cggcggcgcc ctcgagctcg aggctggaat cgagcgcgag
     3481 cgtccagcgg ccggcgggca gcgggaactc gcgcggccgg gattcggcat tgaacagcac
     3541 gagcagcgcg ggccgcggcg gcgcgtcggg cgcggcgacc gggtcgatgc ggcagccgaa
     3601 ggcgcgcacc tcgtcgcggt gccagtcggc cgcatccatc tcgccgccgc gctcgttgag
     3661 ccacgccagc cgcggcgcgc cgccggcacc ggcgcgccac tcgcccacgc gcaggtgcgc
     3721 atgcgcccgg cgcagcgcga gcgcgcgcgt cgtgaaggcg agcatcgtcg cgtccgcggc
     3781 cggccagtcg atccaggcga tcggcgcgtc gtggcagtac gcgttgttgt tgccctgctg
     3841 gccgttgccc atctcgtcgc cggcgcacag catcggcgtg ccttgcgcca gcgccagcgt
     3901 ggccatcatc gcccgtcgca cgcggccgcg cagctcgagg atgcgctcgt cgcgggtggg
     3961 accctccgcc ccgaagttgg cgcagggctc gccgtcgcgg ccgtcgcggt tgccttcgcc
     4021 gttggcctcg ttgtgcttgc gcgcatagct cgtgaggtcg gccagcgtgt agccatcgtg
     4081 cacggcgagg tagttgaccg aggcgagcgg cgtgcgctgg ccgtggtcga agaggtcgct
     4141 cgaggccgtg aagcggcgcg cgagctcgcc gcggccgacg cgctcgggct gcgcgtcgcc
     4201 cagccaccag cggcgcacgg aatcgcggaa cctgtcgttc cattcgagga agcggccggg
     4261 gaagcggccg acctggtagc cgtcgggcgc cgcgtcccag ggctccgcga tcaggtgcac
     4321 gcgcgcgagc accgggtcct ggcgcagcgc ggtgaagaac gcggcgtccg ggtcgaagcc
     4381 gcgcgccgtg cggcccagca cgggcgcgag gtcgaagcga aagccgtcga cgcccatttc
     4441 ctcgacccag tagcgcagcg aatccagcac gaactgcgtc acgcggggat gcgccacgtt
     4501 gaccgtgttg ccgcagccgg tgacgttctc gaagcggccg cggtcgtcgg cgtcgaggcg
     4561 gtaccagctc gcgttgtcca ggccgcggaa gctgatggtg gggccgtgct cgttgccctc
     4621 gggggtatgg ttgtagacga cgtccagcac gacctcgagg ccgtgctcgt gcagcgtcgc
     4681 gaccatctcg cggaactccg cgttgaccgc ggccgggtcg cccgacgtga gcgagaagcg
     4741 cggatccggg cagaagaatc ccagcgtgtt gtagccccag taattggtca ggcccttgcc
     4801 cgcgagcatg ggctcgtcga cgtggtattg caccggcagc agcgacaagg tcgtcacgcc
     4861 cagcgacctg aagtgcgcga tggcggccgg gtgggcgaga cccgcgtagg tgccgcgcag
     4921 ctcgggcggg atccccggct gcagcttgcc gaagcccttg acgtgcagct cgtagagcac
     4981 gacgtcctcc ggcgcgaccc gcggcgcgtt cagccagccg cgcgcggccc gcgccggcgg
     5041 cgcgacgcgc gccttcagcg ccgaggcggc gttgtcgcgc acgtcgaagg aacgcgggcc
     5101 ctcgggatgg ccgagctcgt agccgtggtg ctcggcgcgc caatcgaagc ggccgacgat
     5161 ctcgcgcgcg cacgggtcga gcagcagctt gttcgcattg aagcggtggc cgcaggccgg
     5221 ctcgtacggg ccgtgggcgc gcaggccgta gacctgcccc tcgcccagcc ccggcaggaa
     5281 gccgtggaag acgccgtcat gggggccgcg cagctcgtag cgacggagct cgcgcgcgcc
     5341 ggcggcgtcg aacacgcaga actcgatgcg ctgcgcatgc tgcgagaaga cggcgacgtt
     5401 gacgccgccg tcgcgcgcgt gcgcgcccat cggctcgcaa cgtccgggca agacggtggc
     5461 gggaagctgg agcatcggca aggggcgtga agcaggtacg gggatggggt atcaggcggg
     5521 cagcaggtag accgtcgcga gcggcggcac caccatgcgg atcgactgcg accgcccgtg
     5581 cgacgcgacg gcctcgaccg gcagcaccgc ggacgcattg cccacgttgc tgccgccgta
     5641 gtgcgccgag tcggtgttga ggacctcgcg ccagcccgtc ggcccctccg gcacgcccag
     5701 cacgtagctg aactgcggca cgggggtcat gttgcagacg gcgatcatcg cgtcgccgtc
     5761 ggtgtcgcgg cggatccagg ccagcagcga gttcgtcgcc tcggtggagg cgatccactc
     5821 gaagccgaag gcgtcgcagt cctggcggtg cagcgcgcgt tcctcgcggt acacgcggtt
     5881 caggtcccgc acgaggcgct gcacgccggc gttgcgcgcc tgcccgagga ggtgccaggg
     5941 cagctcggtg tcgtagtccc actcggtgtc ctgcgcgaac tcctggccca tgaacagcag
     6001 cttcttgccg gggtgcgccc acatgaacgc gaagtaggcg cgcaggttgg cgaagcgctg
     6061 cccgtcgtcg ccgggcatgc gcgcgatcag cgagcccttg ccgtgcacca cctcgtcgtg
     6121 cgacagcggc aggatgaagt tctcgtcgaa cgcgtagacc agcccgaacg tgagcttgtc
     6181 gtggtgcagg cggcgctgca cgtgcggctg gcgcatgtac agcagcgtgt cgtgcatcca
     6241 gcccatgttc cacttgaaat ggaagcccag gccgcccgcg taggtcgggt gcgagacgcg
     6301 cgggaaggtc gtcgattcct cggccagggt gatcgcgccg ggggcctcgg cgacgagcac
     6361 ctcgttgatg tgcttgagca gctcgatcgc ctcgaggttc tcgcgcccgc cgtagcggtt
     6421 gggcagccac tcgccggagc tgcggctgta gtcgcggtag atcatcgagg cgaccgcgtc
     6481 cacgcgcagg ccgtcgacgc cgaagcgctc gatccagtac agcgcgctgc cggcgaggaa
     6541 gcagcgtacc tcgggccggc cgaagtcgta gatcagcgtg ttccagtcgc ggtggaagcc
     6601 ctcgcgcgga tcctcgtact cgtacagcgc ggtgccgtcg aagcgcgaca ggccgtgggc
     6661 gtcgatcggg aagtgcgcgg gaacccagtc cagcagcacg ccgatgccgc gcgcatggca
     6721 ggcgtcgacg aagcgctgga aggcccgcgg gtcgccgaag cgcgcggtcg gcgcgtacag
     6781 gcccagcgtc tggtagcccc acgagccgtc gaacgggaac tcggtgatcg gcatcagctc
     6841 gacgtgcgtg aagccgaggt cgctcgcgta ggcgggcagc tcctcggcga gctcgtccca
     6901 ggtcttgaag cggccgtccg cgcggcgcca ggagccggga tgcacctcgt agacggagat
     6961 cggggcgtgg cggtcgttcg cgcgcgcgcg ccaggacggc agcgcgcggc gctcgggcag
     7021 cggcgcgacg atgctggcgg tgtcggggcg cagctgcgcg gcgaacgcgt acgggtcggc
     7081 cttgagcggc tgcagcgcgt catcggggcc gacgagctcg tacttgtagc ggtcgcccgc
     7141 gatcatgtgc gggacgaaca gctcccacac gcccgacggg tcgcgtcggc gcatcaggtg
     7201 gcgacggccg tcccagttgt tgaagtcgcc gaccacgctg acgcggcgcg cgcgcggtgc
     7261 ccacaccgcg aagcgcacgc ccgcgaccgc gcgctcgccc tcgcccagcg ccgtcggatg
     7321 cgcaccgagc agctcgcagg gccgcaggtg gcggccttcc gagaaggcgc gcagcgccgc
     7381 ctcgtccagc aacgggccga aggcgtaggg atcggcgtag tcgtcgcgca cgccggagtc
     7441 ccagcgcacg cgcagccggt agtcctcgac cggcgcgacc aggggcgact cccacacgcc
     7501 cgcgggatgc cggcgatcca gcacgaccac gcgctcgccc gcggcgtcga ccagctcgac
     7561 gtcctcggcg ttgggcagga aggcgcgcac ccaccactgt ccgcgcgcat cggcgcgcac
     7621 gccgaggatc gcgaacggat cgggatggcg cgcccgcacg agcgcgccca ggtcgtcatc
     7681 ggtgagcatg gccccggccc tcgcagacgg tggagatcgt cgggatgacg gcgccgtgct
     7741 cagcggccct cgggcgccga ccagacatgc tgcacgtact cgcggatcgt gcggtcggcg
     7801 ctgaacgcgc ccatgcccgc gacgttgcgc agcgcgcgct cggcccatgc cgccggctgc
     7861 gcgtagagct ggtcgacaag cgactgcgcg tgcaggtagt ccgcgaagtc ggcgagcagc
     7921 atgtacgggt cgcgatgcag cagcggctgc acgagcgact tgtagcgatc gggctcgccc
     7981 cacgagaaga cgccgccccc gatggcgtcg agcacttgct gcagggcctg gttttcttcc
     8041 gcatagagac gcggatcgta tccccacgac ttcatcttcg cgacggcgtc ggcgcgcagg
     8101 ccgaagacga acatgttctc ggtgccgatc gcctcggcca tctcgatgtt ggcgccgtcc
     8161 caggtgccga tcgtgagcgc gccgttgagc gcgaacttca tgttgccggt gcccgaggcc
     8221 tcggtgccgg cggtcgagat ctgctcgctc aggtcggccg ccgggatgat ggcctcggcc
     8281 aggctcacgg agtagttcgg caggaagacg accttgagct tgtcgcccac gcgcggatcg
     8341 gcgttgacca cccgcgcgac gtcgtggatc agctggatga tcagcttggc cgcgtggtag
     8401 gccgaggcgg ccttgcccgc gaagatcacc gtgcgaggcg tccagtcggc gcccgggtcg
     8461 gcgacgatcg cctggtagcg cgcgaccacg tgcagcacgt tgagcagctg gcgcttgtac
     8521 tcgtggatgc gcttgatctg gacgtcgaac aggctcgccg ggtcgatgac cacgccgaac
     8581 tcgcgcttga tgcgcacggc caggcgttcc ttgttcgcgc gcttcgtggc gaggaaggcc
     8641 tgccccagcg ccttgtcggc ggcctgcggc gcgagcgcgg cgagctcgtc cagatcggag
     8701 cgccagcccg tgccgatgcg ctcgtccagc agcgtcgaca gcgcgggatt ggcctgcatg
     8761 agccagcgcc gcggggtcac gccgttggtg acgttgagga agcgctgcgg gaacatgcgc
     8821 gcgaagtccg cgaagatggt ctcgaccatc agccgcgagt gcagcgccga cacgccgttg
     8881 accttgcgcg acgcgacgat cgcgagcgag gccatgcgca cgcggcgctc gcccacctcg
     8941 tcgatcagcg acagccgcgc gatgagcccg ccgtcgcccg ggaagcgctc gccgacctcg
     9001 tcgaggaagc ggcggttgat ctcgtagacg atctcgaggt ggcgcggcag cagctgctcg
     9061 aacatcgtga ccggccaggt ctcgagcgcc tcgggcatca gcgtgtggtt ggtgtagctc
     9121 gtcgcctcgg tcgtgatcgc ccatgcgtcc tcccaggcga ggccgtgcac gtccagcagc
     9181 aggcgcatca gctccgcggg cgcgagcgcg gggtgcgtgt cgttcaggtg gacggcgttg
     9241 tggcggccga agctgcgcac gtcgccgtgc tcgcgcaggt ggcgcgcgag catgtcctgc
     9301 accgatgcgc tcaccaggaa gaactcctgc ttcaggcgca ggaggcggcc cgccgccgtg
     9361 gaatcgtcgg ggtacagcac ccagttcagc gagtcggcgg cctcgagatg ctggcccgcg
     9421 gccaggtgct cgccgcggca gaaggtcgcg aagtcgatcg ggcgcgcggc ctgcgcatgc
     9481 cactggcgca gcgtcgagac gcgctgcgtg gcgtggccgg ggacgacgaa gtcgaccgcc
     9541 tgcgcggcga cctcctcggc cggcttccag gatcgatggc cgcccgagct ctcgacgacg
     9601 ccgccgaagc cgacgaggtg gcgcagctcg ggacgcggga actcccacgg gttgccgtcc
     9661 ttgagccatt cgtcgggcag ctcgacctgc cggccttcgc ggatggcctg cgcgaacatg
     9721 ccgtgcgtat accgcagccc gtatccgaag gacggcagcc cgagcgtcgc gaacgcgtcg
     9781 aggaagcacg ccgccaggcg accgaggccg ccgttgccga gcgcggcgtc gggctcgtgc
     9841 tccagcacct cgccgatcgc gggcaggccc gggtgcgccc ggagcgcgga ctggaggtcg
     9901 ggcgcgagcc ccagcaccgc cagcgcgttg ccgagggcgc ggcccatcag gaactccatc
     9961 gacaggtagt
//
LOCUS       DASVLN010000936         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_210337, whole genome shotgun sequence.
ACCESSION   DASVLN010000936 DASVLN010000000
VERSION     DASVLN010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210337"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VF453_21980"
     CDS             complement(<1..426)
                     /locus_tag="VF453_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEX7690392.1"
                     /translation="MTRIVRSVASFASAWLIAAGPSAAPVAPLAVPDTIAQRVSACGE
                     CHGREGRSTDHGFFPRIAGKPAGYLYNQLDNFRSGRRSNATMTYLVEQLSDDYLREIA
                     GYFGGLGLPYAPPQVAGASAEDLATGEALVRRGDAARGIP"
     gene            complement(473..1195)
                     /locus_tag="VF453_21985"
     CDS             complement(473..1195)
                     /locus_tag="VF453_21985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7690393.1"
                     /translation="MSAVVLIPTTGAPTLRRAVQSVLAQTHETSAYVVCDGEAFRDAT
                     LAALEGLDRARVALCVLPRNTGAGGFYGHRIYAAFAHLVDEDHVLFLDQDNFLAPDHV
                     ASCVELIAARRLQWCHALRNVCTPAGEFVCRDDCESLGRWPAFTGQDLHLVDTSAWCL
                     RREVLLQAAHVWHGGWGQDRVFAQVMMRNFRDFDCTGRYTLQYCLGGNEGSVTREFFE
                     QGNAEMLRRYKRGFPWNARAAG"
     gene            complement(1268..2593)
                     /gene="glcF"
                     /locus_tag="VF453_21990"
     CDS             complement(1268..2593)
                     /gene="glcF"
                     /locus_tag="VF453_21990"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX7690394.1"
                     /translation="MQTQLAPEFAGTPDGQEAEAILRRCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLMKQVLEGAEPTRSTQEHLDRCLTCRNCESTCPSGVEYGHLVEIGRRIV
                     DAKVPRPAGERAKRWLLREGLTSPLFAPALKLGQALRPLVPASLKGKVPARPSGAEAA
                     REALWPQRTHARKVLMLMGCVQPAMRPNINAATARVLDLAGVQAIVPDGAGCCGAVRD
                     HGGDHEGGLADMRRNIDAWWPWVAGLTEAGAVEAIVVNASGCGVAVKDYGHALARDPA
                     YADKARRVSELARDVGELLDGLMPELMARWKLVRERLPADSPEHANAGASALVFHPPC
                     TLQHGQQLRGGVEGILRAFDFDVRLAASESHLCCGSAGTYSVLQPQIATQLRDRKLAA
                     LAAAEAAPRAIVSANIGCIAHLQSGTATPVKHWIELVDERLSADARRMR"
BASE COUNT          367 a          953 c         1062 g          358 t
ORIGIN      
        1 cgggatgccg cgcgcggcgt cgccgcggcg cacgagggcc tcgccggtgg ccaggtcctc
       61 ggcggacgcc ccggcgacct gcggcggcgc gtacggcagg cccaggccgc cgaagtagcc
      121 ggcgatctcg cgcaggtagt cgtcggacag ctgctcgacg aggtaggtca tcgtcgcgtt
      181 gctccggcgg ccggagcgga agttgtccag ctggttgtag aggtagcccg cgggcttgcc
      241 cgcgatgcgc gggaagaagc cgtggtcggt cgaccggccc tcgcggccat ggcactcgcc
      301 gcaggccgag acgcgctggg cgatcgtgtc cgggacggcg agcggcgcga ccggcgccgc
      361 ggacgggccg gcggcgatca gccaggccga ggcgaacgag gcgacggagc ggacgatccg
      421 ggtcatggcg ctcgaccgta cgcggattcg cgccgcggcg ccaccctcgg tttcacccgg
      481 ccgcgcgggc gttccaggga aagccgcgct tgtagcgtcg cagcatctcc gcgttgccct
      541 gctcgaagaa ctcccgggtc accgagccct cgttgccgcc gaggcaatac tgcagggtgt
      601 atcggccggt gcagtcgaag tcgcggaagt tgcgcatcat cacctgcgcg aagacgcggt
      661 cctgtcccca gccgccgtgc cagacgtggg cggcttgcag cagcacctcg cgccgcaggc
      721 accaggcgct ggtgtcgacc aggtgcaggt cctggccggt gaaggcgggc cagcggccga
      781 ggctctcgca gtcgtcgcgg cagacgaact cgcccgccgg cgtgcagacg ttgcgcaacg
      841 cgtggcacca ctgcaggcgg cgcgcggcga tcagctcgac gcagctggcc acgtgatcgg
      901 gcgcgaggaa gttgtcctgg tcgaggaaca gcacgtgatc ctcgtcgacg agatgcgcga
      961 acgccgcgta gatgcgatgg ccgtagaagc cgcccgcgcc cgtgttgcgc gggagcacgc
     1021 acagggcgac gcgcgcgcgg tcgaggccct cgagcgcggc cagcgtggcg tcccggaacg
     1081 cctcgccgtc gcagacgacg taggccgagg tctcgtgggt ctgggcgagc acgctctgga
     1141 cggcgcggcg cagcgtgggc gcgccggtgg tggggatgag gacgacggcg gacatgcgtg
     1201 gatggtagcg actacctcgc cggcgaacgc tcggccgctc cggagagagc cggcgttcgt
     1261 gtggtcgtca cctcatgcgg cgagcgtcgg ccgagagtcg ctcgtcgacc agctcgatcc
     1321 agtgcttcac cggcgtcgcc gttccggatt gcaggtgcgc gatgcagccg atgttggcgg
     1381 agacgatggc ccgcggcgcg gcctcggcgg ccgccagggc cgcgagcttg cggtcgcgca
     1441 gctgcgtcgc gatctgcggc tgcagcaccg agtacgtgcc cgcggagccg cagcacaggt
     1501 ggctttcgct cgcggccagg cggacgtcga agtcgaacgc gcgcaggatg ccctcgacgc
     1561 cgccgcgcag ctgctggccg tgctgcaagg tgcacggcgg atggaagacg agcgcggagg
     1621 cgccggcgtt cgcgtgctcg ggcgagtcgg cgggcaggcg ctcgcgcacg agcttccagc
     1681 gcgccatcag ctcgggcatc aggccatcga ggagctcgcc gacgtcccgg gcgagctcgc
     1741 tcacgcggcg cgccttgtcg gcgtaggccg gatcgcgcgc cagcgcgtgg ccatagtcct
     1801 tgaccgcgac cccgcagccc gacgcgttga cgacgatcgc ctcgaccgcg cccgcctcgg
     1861 tgagtcccgc gacccagggc caccaggcat cgatgttgcg gcgcatgtcg gccaggccgc
     1921 cctcgtggtc gccgccgtgg tcgcgcaccg cgccgcagca gccggcgccg tcggggacga
     1981 tcgcctgcac gcccgcgagg tcgagcacac gtgcggtggc ggcgttgatg ttgggccgca
     2041 tcgccggctg cacgcagccc atcagcatca gcaccttgcg cgcgtgggtg cgctgcggcc
     2101 acagggcctc gcgcgcggcc tcggcgccgg agggtcgtgc cggcaccttg cccttcagcg
     2161 acgccggaac cagcggccgc aaggcctggc cgagcttcag cgccggcgcg aacagcggcg
     2221 aggtcagccc ctcgcgcagc agccagcgct tcgcgcgctc gcccgcggga cgcggcacct
     2281 tggcgtcgac gatgcggcgg ccgatctcga cgaggtgccc gtactcgacg cccgaggggc
     2341 aggtcgattc gcagttgcgg caggtcaggc agcggtcgag gtgctcctgc gtcgagcgcg
     2401 tcggctccgc cccttcgagc acctgcttca tcaggtagat gcgcccgcgc gggccgtcga
     2461 gctcgtcgcc gagcagctgg taggtcgggc aggtcgcggt gcagaagccg cagtgcacgc
     2521 agcggcgcag gatggcctcg gcctcctggc catcgggggt gccggcgaat tcgggggcga
     2581 gctgggtctg catcgggaga cgggttcaga agacgaccgg gcggcccggc acgaagacgc
     2641 cgcgcgggtc gaaggcgtcg cgcaggcggc gctgcacttc gagcaccgcc ggcgccggct
     2701 tcgtcgggtt cagggcgccc ttgccgagcg ggccgccgag
//
LOCUS       DASVLN010000937         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_264870, whole genome shotgun sequence.
ACCESSION   DASVLN010000937 DASVLN010000000
VERSION     DASVLN010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264870"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1103)
                     /locus_tag="VF453_21995"
     CDS             complement(96..1103)
                     /locus_tag="VF453_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690395.1"
                     /translation="MQGTLIGSPFSTGLSYDAATLDLATGAVAGLPRSDESLSTDGAT
                     DQWTANSAAASTSDLVRVDRLGDVDFFDRVTHAHTGGFALSAIAGTYHPGLWSMLKPS
                     PDGRYLLGYWKPDSTAQDATLAVFDRNGNVVQQGSPVDYDVASYLFAFDWMPDGRYVF
                     LAGDNLVVATPGNVNATVTPLALPAGVNTPGSEIAVSPDGTKLALKLGIDMTDQVGNA
                     VGRGVLFISDLAVTNLHQLTTLSARAQASAGAIGHSNPVWSPDGQYVAFTIAYPQAGL
                     GDSPSGCPPTYVLPVSSDTVAIDDISDPDSERFMVVNPATNATVPVHACYENMSWISS
                     S"
     gene            complement(1375..2088)
                     /locus_tag="VF453_22000"
     CDS             complement(1375..2088)
                     /locus_tag="VF453_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690396.1"
                     /translation="MSGALSSVMDSVTGGAMGGSLLGDIGGLLGSEFGPIGSMIGEAI
                     GNALQQVVQNAVSQAMQQLQSQGMPSFVAQAVNDKTSQVVAQNTNPNVPAAAQQHVQD
                     QYGAELTDLTNQTGAFIAASAAEKHKNSSKATGGAGGWLEAIAQAMGEALGQQASALV
                     GLSNKLSGEVQTPSTGSGQSGASTGGNQASNTAQAQQFNEDMTQFQALSQQYSILQNT
                     FTNAIQAIGTALQGMARKG"
     gene            2489..>2684
                     /locus_tag="VF453_22005"
     CDS             2489..>2684
                     /locus_tag="VF453_22005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690397.1"
                     /translation="MIDNDFTAVAPNARDLLDKLAADAPAAAGDAPPDGVPDALWRPL
                     TDAERALVRNLPGTTQSAPAA"
BASE COUNT          358 a          941 c          959 g          426 t
ORIGIN      
        1 gaccgccggg gcaccgcagc acgcgccggc cgccagcgcc agggccgcga ggcggccgcg
       61 ggttcggcgc cgcgcggccg ggacgatggc ttcgatcagc tcgacgagat ccatgacatg
      121 ttttcgtagc acgcgtgcac cggcacggtc gcgttcgtgg ccgggttgac gaccatgaag
      181 cgctcgctgt cgggatcgct gatgtcgtcg atcgcgacgg tgtccgagga taccggcagc
      241 acgtacgtcg gggggcatcc gctcggcgag tcgcccaggc ccgcctgcgg gtaggcgatc
      301 gtgaatgcga cgtactgacc gtcgggcgac cagaccgggt tcgaatgccc gatcgcgccc
      361 gcggaggcct gcgcacgcgc gctgagggtg gtcagctggt gcaggttcgt gacggcgagg
      421 tcggagatga acagcacgcc gcggccgacc gcgttgccga cctggtccgt catgtcgatg
      481 cccagcttga gcgcgagctt cgtgccatcg gggctgaccg cgatctccga gcccggcgtg
      541 ttgacgccgg ccggcagcgc gagcggcgtg acggtcgcgt tcacgttgcc cggcgtggcg
      601 acgaccaggt tgtccccggc gaggaagacg tagcggccgt cgggcatcca gtcgaacgcg
      661 aacaggtagc tcgcgacgtc gtagtccacc ggcgagcctt gctgtacgac gttgccgttg
      721 cggtcgaaca ccgcgagcgt cgcgtcctgg gccgtcgagt cgggcttcca gtaccccagg
      781 aggtatcgac cgtccgggga gggcttgagc atgctccaca ggccgggatg gtaggtgccg
      841 gcgatggccg acagcgcgaa gccgcctgtg tgcgcgtggg tgacgcggtc gaagaagtcg
      901 acgtcgccga ggcggtcgac gcgcacgagg tcgctggtcg aggcggccgc ggagttcgcg
      961 gtccactggt cggtcgcgcc gtcggtcgac agcgactcgt cgctgcgcgg caggcccgcg
     1021 acggcgccgg tggcgaggtc gagcgtcgcg gcgtcgtagc tcaatccggt gctgaagggg
     1081 ctgccgatca gcgtgccctg catggcgccg gtcgggccac caccgccacc gccgccagcg
     1141 ccaccgccag cgccaccgcc accgccaccc gcgctgggcg tgccgctgtc gcctccgccg
     1201 ccgcaggcgc tcacgcccag ggcggcgacc acgactgcga ggcgcgccag gcctgcaagg
     1261 ccaccggtcc gttcgatacc catttccgat ctcccaaatg caaatcggcg agcagccgac
     1321 ggctgctcgc cccgcgcgac gcccgagggc gccgcgagcg tccgatgtcg aggatcaacc
     1381 cttgcgagcc atgccttgca gcgcggtgcc gatcgcctgg atggcgttcg tgaacgtgtt
     1441 ctgcaggatg ctgtactgct ggctcagcgc ctggaactgc gtcatgtcct cgttgaactg
     1501 ctgggcttgc gcggtgttcg aagcctggtt gccgcccgtc gacgcgccgg actggccgga
     1561 gccggtgctc ggggtctgga cttcgcccga cagcttgttg gacaggccga ccagcgccga
     1621 ggcctgctgg cccagggcct cgcccatggc ttgcgcgatc gcctccagcc agccgccggc
     1681 gccgccggtc gccttgctgc tgttcttgtg cttctcggcg gccgaggcgg cgatgaacgc
     1741 tccggtctgg ttggtcaggt cggtgagctc ggcgccgtac tggtcctgca cgtgctgttg
     1801 agcggcggcc ggaacgttcg ggttcgtgtt ctgcgcgacg acctggctgg tcttgtcgtt
     1861 gaccgcctgg gccacgaacg agggcatgcc ctgcgactgc agctgctgca tggcctgcga
     1921 cacggcgttc tgcacgacct gctgcagggc gttgccgatg gcctcgccga tcatcgagcc
     1981 gatcgggccg aactcgctgc ccagcaggcc gccgatgtcg cccagcaggc tgccgcccat
     2041 ggcgccgccg gtcacgctgt ccataacact gctcaaagct ccggacattt cgatctcctt
     2101 gttggcgccg acgaggcgcc ggggttcatt gaactgatca gcggccggct gcaggttcct
     2161 ccctgtcgcc tcgggcggcg ctcctttctc ggatacaccc cccggtacca ctcgcgatgt
     2221 ctgccgcctt gccggcgcgc cctgcggggc tcgctgtgca ggatcgacgg aacgaattaa
     2281 aggcttcgct gcggactttc gtaagctggg tcggttccca ggtgggaatc gacccagcgc
     2341 gcgtgggcgc gctgggaagc gtcccagctt gtggacgtcg cggcgcattc ctaccctggc
     2401 cgtccgtgcc aggaaggacg cgtgcgcggc gcgcacgcac aacgcacgcg cacggccttc
     2461 gccgcgcgca tcgccaggga gctctccgat gatcgacaac gacttcaccg ccgtcgcgcc
     2521 aaacgcgcgc gacctgctcg acaagctcgc ggccgacgcg cccgcggccg ccggcgacgc
     2581 cccgccggac ggcgtgcccg acgcgctctg gcgacccctc acggatgcgg agcgtgcgct
     2641 cgtgcgcaac ctgccgggga ccacccagtc cgcgcctgcg gcgg
//
LOCUS       DASVLN010000938         8461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_47477, whole genome shotgun sequence.
ACCESSION   DASVLN010000938 DASVLN010000000
VERSION     DASVLN010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47477"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..853
                     /locus_tag="VF453_22010"
     CDS             233..853
                     /locus_tag="VF453_22010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7690398.1"
                     /translation="MFQRTISTLILAAATFGATAAFAQTAPDAMIKEVAGDVIDAVKG
                     DKAIQAGDVSRIHGLVDTKVMPHVDFQRMTAATVGRAWRSATPEQKDKLQAEFKTLLI
                     RTYAGALTRVNASTTVDLKPSRGAAEGDDATVRTEIKGNGDPVEIDYRLEKMPSEWKI
                     YDVNVLGVWLVDQYKSSFQQQIQQGGLDGLISTLQAKNKGASAPAK"
     gene            850..1260
                     /locus_tag="VF453_22015"
     CDS             850..1260
                     /locus_tag="VF453_22015"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7690399.1"
                     /translation="MSDGRVDSTATSPAKTAAGRELATALPASLTLKDATPVLASLRA
                     AFEGDGGGTWRVDASPLEKLDTSALAVLLECARIAAAGGRRLEIVGAPARLSDLAHLY
                     GVDGLLGILHDANEPVAGIVPSKPTEAPTVPILR"
     gene            complement(1363..2229)
                     /locus_tag="VF453_22020"
     CDS             complement(1363..2229)
                     /locus_tag="VF453_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX7690400.1"
                     /translation="MLERVRASIPALPPAEQRVAKLVLSDPRSFASMPVSELAERSHV
                     SKPTVVRFCRSVGYDGLADFKLKLAGTVNEGVPFVHRSVDEDDGPGELIVKVIDNAVS
                     AMLKYRNNAASHAFERAIAALTQAGLEKKRIDFYGVGNSGIVAQDAQHKFFRLGVNTS
                     ASSDGHVQVMSATMLGPGDCAVVISNSGRSRDLIDVADIARRKGATLIVITASSSPLA
                     QMTQANSQILLAADHPEDFDRYSPMVSRMLHLMIVDILTTGVALRLGQGLRPVLQEIK
                     RNLRARRYAMPE"
     gene            complement(2350..3807)
                     /gene="zwf"
                     /locus_tag="VF453_22025"
     CDS             complement(2350..3807)
                     /gene="zwf"
                     /locus_tag="VF453_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7690401.1"
                     /translation="MSFDLVFFGGTGDLTWRKLMPALFQAFRHGKLPEGGRILAVARD
                     ERTDEEYRNFIRERFVEVESSKRPTDEEFARFAELLHYRRMDLSKPDDYAGLKAWIDA
                     RGADTAVLYLATSPHLFPAICEQLGQAGLNGPHVRVVLEKPLGHDLASALEINRVVKS
                     VFEERQAFRIDHYLGKPSVQNLMALRFGNALFEPLWNRQSIANIQITLAESLGVGTRG
                     DFYDRTGTLRDMVQNHALQLLTMIAMEPPATNDADAIRDEKLKVLRSLKPFTAESVAR
                     DVVRGQYRAGSIDGRAVPGYLDEVKVPPGSRTETFLALRTEVQNWRWAGVPFYIRTGK
                     RLAARDAQIVVNFRPVPHPIFSGPNRANQLVIKLQPEDGLELTLQAAKGGLATAGMGE
                     SLSTVSLDLDFDKAFPSERVGAYERLLLDAIAGRLNLFVRSDEQEQAWRWVGPILDAW
                     ANDSEPPRSYAAGSWGPAAASSLIARDGFAWAEEE"
     gene            4046..4999
                     /gene="tal"
                     /locus_tag="VF453_22030"
     CDS             4046..4999
                     /gene="tal"
                     /locus_tag="VF453_22030"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX7690402.1"
                     /translation="MNQLEQLKSSTVVVADTGNFKQMAQYAPRDATTNPSLILKAVQN
                     ADYAPLLADTAAAHAGKALPDLVDQVLVRFGLEILKVVPGRVSTEVDARLSFDTAATV
                     ARARRIMGLYEAAGIGRDRVLIKIASTWEGIQAARQLEAEGIHCNLTLLFSFCQAVAC
                     GDAGVRLISPFVGRIYDWYKKSAGAGWDEAAKAGANDPGVQSVTRIFNYYKHFGIATE
                     VMGASFRNVGQIRALAGCDLLTISPELLAQLQADETPFERTLDAQGAKALPVERVRFD
                     EPGFRFALNEDAMATEKLAEGIRAFAADSVKLDALIQAGQR"
     gene            4996..6648
                     /gene="pgi"
                     /locus_tag="VF453_22035"
     CDS             4996..6648
                     /gene="pgi"
                     /locus_tag="VF453_22035"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX7690403.1"
                     /translation="MSPATADARFPRCDRTAAWKALRAAFDGGAKDFDMRTAFVGDAT
                     RFERFAVEAPGVFGDLSKHRWDEATRGLLLQLARECELEAHRDAMFAGEPINLTEGRA
                     VLHTALRTPAGAPSISAATDAAVKEVHSVLEPMLAWAESVRAGAGTRFTDVVNIGIGG
                     SDLGPAMAVLALDEFVHPKLRFHYVSNVDGHDIAPVLARLDADRTLFIVASKTFTTQE
                     TLANAHVARDWFLAHGGRDVSANFVGVTTNLEAAAKFGVTTTFGFWDWVGGRYSLWSA
                     IGLSIAIAIGADRFRDFLAGAHAMDEHFRTAPLESSLPVQLALVDIWYRNFHGFTSRS
                     VAPYHQGLKRFPAYLQQLEMESNGKRVDVDGAVVPFGTSPVVWGEPGTNGQHAYFQML
                     HQGTDVVPVEFIAVKRPAAGHETGALADLHAKLLANCLAQSQALMQGKSAEEAAAESP
                     PTASKTIARDVIARHRSFPGNRPSTTLVLDALTPRTLGALIALYEHRVFCTGALWGIN
                     SFDQWGVELGKALANELLPRLASGDAAGLDGSTAGLLARLRG"
     gene            6706..7341
                     /locus_tag="VF453_22040"
     CDS             6706..7341
                     /locus_tag="VF453_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7690404.1"
                     /translation="MNDIPNSDRAFVFDFGRVLFRWRPDAVVAQTLAHRLDAQRDVAH
                     WVEQVFQAYDGDWQYFDRGTVEPPELARRIAGRTGLAEADVLGLIAACPGELQPIPET
                     VAWLRRLHAQGRPLHYLSNMPEPFAAHFERTHDFMRLFESGVFSARVQLVKPEPEIFE
                     HAARVFGRAPGALLFLDDHAPNIAAARAAGWQAILFEDAAQAQAEVAALGW"
     gene            7422..7802
                     /locus_tag="VF453_22045"
     CDS             7422..7802
                     /locus_tag="VF453_22045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7690405.1"
                     /translation="MPKIMVVDDLRDSADSLALLFDALGHTTLTSYDGQQAVEAAEDF
                     APDIVFLDINMPVLDGYGAARALRKLLVDAPPVLVALTAVSGPDAKRRADEAGFDFYV
                     TKPADFNVLITIVDDLSRRIDARH"
     gene            complement(7796..>8461)
                     /locus_tag="VF453_22050"
     CDS             complement(7796..>8461)
                     /locus_tag="VF453_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7690406.1"
                     /translation="PALARPGARHRLLTVCSGALLAADAGLVGDRRVTTHHELLDSIG
                     ALAPRAQVCANRVFVVDGPLWSSAGITTGIDLALHCIGDVCGEAVAASVAQTMVVFLR
                     RAADDPELSPMLAGRGHLHPAVHRVQNAVCERPAADWDVPALAEVAHVTPRHLARLFK
                     EHAGVSPRDYVEQVRRGLAEQALARGLTTSQALELAGFGSDRQWRRARARHRAAQGGT
                     ALQ"
BASE COUNT         1218 a         3045 c         3007 g         1191 t
ORIGIN      
        1 ccgcgcagcc ggcgccgatg caggcctcgg caccgccttt gcctgtcccg ttgccgggcc
       61 ccgcgcccgt gccgccgccg ccggcgtccg ccccgtcggg cagctgagac aatgcggcgt
      121 cgcgtccggc ctgcccggcc ggggcaacgt tactttgcac ggccggtgtg tgaaccgccc
      181 cgggcaagct tgcgttaagg agaagagcga acccccgctc gaaaggacac gaatgtttca
      241 acgcacgatt tccacgctga tcctcgccgc tgccaccttc ggcgccaccg cggcgttcgc
      301 gcagaccgcg ccggacgcga tgatcaagga ggtcgccggc gacgtcatcg acgccgtcaa
      361 gggcgacaag gccatccagg cgggcgacgt ctcgcgcatc catgggctcg tcgataccaa
      421 ggtcatgccg cacgtcgact tccagcgcat gactgccgcg acggtcggcc gcgcgtggcg
      481 ctcggcgacg cccgagcaga aggacaagct gcaggccgaa ttcaagacgc tgctgatccg
      541 cacgtacgcc ggtgcgctga cccgcgtgaa cgcgagcacg accgtcgacc tgaagcccag
      601 ccgcggcgcc gccgagggcg acgatgcgac cgtccgcacc gagatcaagg gcaacggcga
      661 cccggtcgag atcgactacc gcctcgagaa gatgcccagc gagtggaaga tctacgacgt
      721 caacgtgctg ggcgtgtggc tcgtcgacca gtacaagagc agcttccagc agcagatcca
      781 gcagggcggc ctcgacggcc tgatctcgac gctgcaggcg aagaacaagg gcgcctcggc
      841 ccccgccaag tgagcgacgg ccgcgtggac agcaccgcga cgtcgccggc gaagacggcg
      901 gccgggcgcg aactcgcgac ggccctgccg gcgtcgctga cgctgaagga cgccacgccg
      961 gtgctcgcgt cgctgcgcgc ggcgttcgag ggcgacggcg gcggcacgtg gcgcgtggat
     1021 gcgtcgccgc tggagaagct cgacacgtcg gcgctggcgg tgctgctcga atgcgcgcgc
     1081 atcgccgccg cgggcgggcg ccggctcgag atcgtcggag caccggcgcg tctgtccgac
     1141 cttgcgcacc tgtacggcgt cgacggcctg ctgggcatcc tgcacgacgc caacgagccg
     1201 gtcgccggga tcgtcccgtc caagccgacc gaggcgccta ccgtgccgat cctgcgctga
     1261 cgccacgggg actcgtcgcc cgctcccgaa ggcccgccgc gcgcgggcct tcgcattccc
     1321 gggctgccgc gaaaagcgga cggcccgcgt gcggcggccg gctcactcgg gcatcgcgta
     1381 gcgccgggcg cgcaggttgc gcttgatctc ctgcagcacc ggtcgcaggc cctggccgag
     1441 gcgcagggcg acgccggtgg tcaggatgtc gacgatcatc aggtgcagca tgcgcgacac
     1501 catggggctg tagcggtcga agtcctcggg gtggtcggcc gccagcagga tctgcgagtt
     1561 ggcctgcgtc atctgcgcca gcggcgagct gctggccgtg atcacgatca gcgtcgcgcc
     1621 cttgcggcgg gcgatgtcgg cgacgtcgat cagatcgcgg ctgcgacccg agttgctgat
     1681 gaccaccgcg cagtcgcccg ggcccagcat cgtcgcgctc atcacctgca cgtggccgtc
     1741 gctggaggcc gaggtgttga cgccgaggcg gaagaacttg tgctgcgcgt cctgcgccac
     1801 gatgcccgag ttgccgacgc cgtagaagtc gatccgcttc ttctccaggc cggcctgcgt
     1861 gagcgcggcg atcgcgcgct cgaacgcgtg gctggccgca ttgttgcggt acttcagcat
     1921 cgcgctgacc gcgttgtcga tcaccttgac gatgagctcg cccgggccat cgtcctcgtc
     1981 gaccgagcgg tgcacgaacg gcacgccctc gttgacggtg ccggcgagct tgagcttgaa
     2041 gtccgctagg ccgtcgtagc cgacgctgcg gcagaagcgc accaccgtcg gcttggagac
     2101 gtgcgagcgc tccgccagct cgctcacggg catcgacgcg aagctgcgcg ggtcgctcag
     2161 cacgagcttg gcgacgcgct gttccgccgg cggcagcgcg ggaatcgacg cgcgcacgcg
     2221 ctcgagcatc gtcatgtcgt gaccgggtga catgtcagga gacggatgca agcgcgcggc
     2281 cggcgcggcc gaccatgcag ggccgcaccg cgccgggcgg caggctcatt tccggtgacg
     2341 acgcgtcggt cactcttctt ccgcccacgc gaagccgtcg cgcgcgatca gcgaactggc
     2401 ggccgccggg ccccagctgc cggccgcgta ggagcgcggc ggctcggagt cgttggccca
     2461 tgcgtcgagg atcggcccga cccagcgcca ggcctgctcc tgctcgtcgc tgcgcacgaa
     2521 caggttcagg cggccggcga tcgcgtcgag cagcaggcgc tcgtaggcgc cgacgcgctc
     2581 gctcgggaag gccttgtcga agtcgaggtc cagcgagacc gtgctgagcg actcgcccat
     2641 gcccgcggtc gcgaggccgc ccttggccgc ctgcagcgtg agctcgaggc cgtcctccgg
     2701 ctgcagcttg atgacgagct ggttggcgcg gttcgggccc gagaagatcg gatgcggcac
     2761 cggccggaag ttgacgacga tctgcgcgtc gcgcgcggcc agccgcttgc cggtgcggat
     2821 gtagaagggc acgccggccc agcgccagtt ctgcacctcg gtgcgcagcg cgaggaaggt
     2881 ttcggtgcgg ctgcccggcg gcaccttgac ttcatcgagg tagcccggga cggcgcggcc
     2941 gtcgatgctg ccggcgcggt actggccccg cacgacgtcg cgcgcgacgc tctcggccgt
     3001 gaagggtttg agcgagcgca gcaccttcag cttctcgtcg cggatcgcgt cggcgtcgtt
     3061 ggtggcgggc ggctccatcg cgatcatcgt cagcagctgc agcgcgtggt tctgcaccat
     3121 gtcgcgcagc gtgccggtgc ggtcgtagaa gtcgccgcgc gtgcccacgc ccagcgactc
     3181 ggccagcgtg atctggatgt tggcgatgct ctggcggttc cacagcggct cgaacagggc
     3241 gttgccgaag cgcagcgcca tcaggttctg caccgagggc ttgccgaggt aatggtcgat
     3301 gcgaaaggcc tggcgctcct cgaacaccga cttcacgacg cggttgatct ccagcgcgct
     3361 cgcgaggtcg tggcccagcg gcttctccag caccacgcgc acgtgcgggc cgttcaggcc
     3421 ggcctggccg agctgctcgc agatcgcggg gaacaggtgc gggctcgtcg cgaggtagag
     3481 cacggcggtg tccgcgccgc gggcgtcgat ccaggccttg aggccggcgt agtcgtccgg
     3541 cttcgacagg tccatccggc ggtagtgcag cagctcggcg aagcgcgcga attcctcgtc
     3601 ggtggggcgc ttggagctct ccacctcgac gaagcgctcg cggatgaagt tgcggtactc
     3661 ctcgtcggtg cgctcgtcgc gcgcgacggc gaggatgcgg ccgccctcgg gcagcttgcc
     3721 gtggcggaag gcctggaaca gcgcgggcat cagcttgcgc cacgtgaggt cgccggtgcc
     3781 gccgaagaag acgaggtcga aggacatatg cgcaggttgg agcagggaac gcgcccgggc
     3841 tgggcggcgc gggtgccgga gccggacttg ccgggagcgc cgcgtgcctg caggagcgta
     3901 gtggtgatgt aactttgttt cagcaataat gatgcacaag tttcttgacg cggtcaattc
     3961 cgcgccgccc ggtgacccgc ccgcaagagg tgtcgaatat ggggtccggc gcgaccgcgt
     4021 tcctttccca gcccatgagg actccatgaa ccagctcgag caactcaagt cgtcgaccgt
     4081 cgtggtcgcc gacaccggca acttcaagca gatggcgcag tacgcgccgc gcgacgcgac
     4141 gaccaacccg tcgctcatcc tgaaggcggt gcagaacgcg gactacgcgc cgctgctggc
     4201 cgacacggcc gccgcgcacg ccggcaaggc gctgcccgac ctggtcgacc aggtgctcgt
     4261 gcgcttcggc ctggagatcc tgaaggtcgt gcccggccgc gtcagcacgg aggtcgacgc
     4321 gcgcctgtcc ttcgacaccg ccgccaccgt cgcgcgcgcc cgccgaatca tgggcctgta
     4381 cgaagccgcg ggcatcgggc gcgaccgcgt gctgatcaag atcgcctcga cgtgggaggg
     4441 aatccaggcc gccaggcagc tcgaggccga gggcatccac tgcaacctca cgctgctgtt
     4501 cagcttctgc caggcggtgg cctgcggcga cgcgggcgtg cggctgatct ccccgttcgt
     4561 cggccggatc tacgactggt acaagaagtc cgccggtgcg ggctgggacg aggccgccaa
     4621 ggcgggcgcc aacgaccccg gcgtgcagtc ggtgacgcgc atcttcaact actacaagca
     4681 cttcggcatc gccaccgagg tgatgggcgc gagcttccgc aacgtcggcc agatccgcgc
     4741 gctcgcgggc tgcgacctgc tgacgatcag ccccgagctg ctcgcccagc tgcaggccga
     4801 cgagacgccg ttcgagcgca cgctggacgc gcaaggcgcg aaggcgctcc cggtcgagcg
     4861 cgtgcgattc gacgagccgg gcttccgctt cgccctcaac gaggatgcga tggccaccga
     4921 gaagctcgcc gagggcatcc gcgcgttcgc ggcggactcg gtcaagctcg acgcgctgat
     4981 ccaggcgggg cagcgatgag ccccgcgacc gccgacgcgc gcttcccgcg ctgcgaccgc
     5041 acggccgcct ggaaggcgct gcgagcggcc ttcgacggcg gcgcgaagga cttcgacatg
     5101 cgcacggcgt tcgtaggcga tgcgacgcgc ttcgagcgct tcgcggtcga ggcgccgggc
     5161 gtgttcggcg acctgtccaa gcaccgctgg gacgaggcga cgcgcggcct gctgctgcag
     5221 ctcgcgcgcg aatgcgagct cgaggcccat cgcgacgcga tgttcgcggg cgagccgatc
     5281 aacctgaccg agggccgcgc cgtgctgcac accgcgctgc gcacgcccgc cggcgcgccg
     5341 tcgatcagcg ccgcgacgga cgccgccgtg aaggaggtgc actccgtcct cgagccgatg
     5401 ctcgcctggg ccgagtccgt gcgcgccggc gcgggcacgc gcttcaccga cgtcgtcaac
     5461 atcggcatcg gcggctccga cctcgggccc gcgatggcgg tgctggcgct cgacgagttc
     5521 gtccatccga agctgcgttt ccactacgtc tccaacgtcg acggccacga catcgcgccg
     5581 gtgctcgcgc ggctggacgc ggacaggacg ctgttcatcg tcgcctccaa gaccttcacg
     5641 acgcaggaga cgctcgccaa cgcgcacgtc gcgcgcgact ggttcctcgc ccacggcggc
     5701 cgggacgtgt ccgccaactt cgtcggcgtg acgaccaacc tcgaggccgc ggcgaagttc
     5761 ggcgtgacga cgaccttcgg cttctgggac tgggtcggcg ggcggtactc gctctggagc
     5821 gcgatcgggc tgtcgatcgc gatcgccatc ggcgccgacc gtttccgcga cttcctcgcc
     5881 ggcgcgcatg cgatggacga gcacttccgc accgcgccgc tggagtcgag cctgccggtg
     5941 cagctcgcgc tcgtcgacat ctggtaccgc aacttccacg gcttcacgag ccgcagcgtc
     6001 gcgccctacc accagggcct gaagcgcttc cccgcctacc tgcagcagct ggagatggag
     6061 tccaacggca agcgcgtgga cgtggacggc gccgtcgtgc ccttcggcac cagcccggtc
     6121 gtctggggcg agccgggcac caacggccag catgcgtact tccagatgct gcaccagggc
     6181 accgacgtcg tgcccgtgga gttcatcgcc gtcaagcgtc ccgccgcggg ccacgagacc
     6241 ggcgcgctgg ccgacctgca cgccaagctg ctcgccaact gcctcgcgca gtcgcaggcg
     6301 ctgatgcagg gcaagtcggc cgaagaggcc gccgcggagt cgccgccgac ggcctcgaag
     6361 accattgcgc gcgacgtcat cgcccgccac cgcagcttcc ccggcaaccg cccgagcacg
     6421 acgctcgtgc tggacgcgct gaccccgcgc acgctcgggg cgctgatcgc gctgtacgag
     6481 caccgcgtgt tctgcacggg cgcgctgtgg ggcatcaaca gcttcgacca gtggggcgtg
     6541 gaactcggca aggcgctcgc caacgaactg ctgccgcgcc tggccagcgg cgacgccgcc
     6601 gggctggacg gctcgacggc cggcctgctc gcccgccttc gcggctgacc ggccctcacc
     6661 ccggcggcgg ccagcccggc cgccgccatc tctcgcattc ccgcgatgaa cgacatcccc
     6721 aattccgacc gcgccttcgt cttcgacttc ggccgcgtgc tgttccgctg gcgccccgac
     6781 gcggtggtcg cgcagacgct ggcccaccgc ctcgacgccc agcgcgacgt ggcgcactgg
     6841 gtcgagcagg tgttccaggc ctacgacggc gactggcagt acttcgaccg cggcaccgtc
     6901 gagccgcccg agctcgcgcg ccggatcgcc ggccgcaccg gcctcgccga ggcagacgtg
     6961 ctcggcctga tcgccgcctg cccgggcgag ctgcagccca tccccgagac ggtcgcctgg
     7021 ctgcgcaggc tgcacgcgca gggccggccg ttgcactacc tgtcgaacat gcccgaaccc
     7081 ttcgctgcgc acttcgagcg cacgcacgac ttcatgcgcc tgttcgaatc gggcgtgttc
     7141 tcggcgcggg tgcagctggt caagcccgag ccggagatct tcgagcacgc ggcgcgggtc
     7201 ttcggccgcg ctcccggcgc gctgctgttc ctggacgacc acgcgcccaa catcgcggcg
     7261 gcgcgcgcgg ccgggtggca ggcgatcctg ttcgaggacg ccgcgcaggc gcaggccgag
     7321 gtcgcggcat tgggctggta gctcccgcgc gcgggccacc gtcgcgtacg acccccgcgc
     7381 cgggttccca ccggatgccc gggacgaagt aagctcccca catgccaaag atcatggtcg
     7441 tggacgacct ccgcgacagc gccgacagcc tggcgctcct gttcgacgcg ctcgggcaca
     7501 cgacgctcac ctcctacgac ggccagcagg cggtcgaggc cgcggaggac ttcgcgcccg
     7561 acatcgtgtt cctggacatc aacatgcccg tcctggacgg ctacggcgcc gcgcgggcgc
     7621 tgcgcaagct gctcgtggac gcgcccccgg tgctggtggc gctgaccgcg gtctccgggc
     7681 cggacgccaa gcgccgcgcc gacgaggccg gcttcgactt ctacgtcacc aagccggcgg
     7741 acttcaacgt cctcatcacc atcgtcgacg acctgtcgcg gcgcatcgac gcgcgtcact
     7801 gaagcgcggt gccgccctgc gcggcccggt ggcgggcccg cgcgcggcgc cactggcggt
     7861 cgctgccgaa gccggcgagc tcgagcgcct gcgaggtcgt caggccgcgg gcgagcgcct
     7921 gctccgcgag gccgcgccgc acctgctcca cgtagtcgcg cggcgagacg ccggcatgct
     7981 ccttgaacag gcgcgcgagg tggcgcggcg tgacgtgcgc gacctccgcg agcgccggca
     8041 cgtcccagtc ggcggcggga cgctcgcaga cggcgttctg gacgcgatgc acggccggat
     8101 gcaggtggcc gcggcctgcg agcatcggcg agagctccgg atcgtccgcg gcccggcgca
     8161 ggaacacgac catcgtctgc gcgacgcttg cggcgacggc ctcgccgcag acgtcgccga
     8221 tgcagtgcag cgcgaggtcg atgccggtgg tgatgcccgc gctcgaccac agcggcccgt
     8281 cgacgacgaa cacgcggttg gcgcagacct gcgcgcgcgg cgcgagggcg ccgatcgagt
     8341 cgagcagctc gtggtgcgtc gtgacgcggc gatcgccgac caggccggca tcggcggcca
     8401 gcagcgcgcc cgagcagacg gtcagcagcc ggtggcgcgc gcccggccgc gccagcgcgg
     8461 g
//
LOCUS       DASVLN010000939         4763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_104042, whole genome shotgun sequence.
ACCESSION   DASVLN010000939 DASVLN010000000
VERSION     DASVLN010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104042"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1752)
                     /locus_tag="VF453_22055"
     CDS             complement(94..1752)
                     /locus_tag="VF453_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEX7690407.1"
                     /translation="MNGSTAASASGGLLSKERTIAGAGFNRWMVPPAALAIHLCIGMA
                     YGFSVFWLPLSKLLAGAPACPKGGILEGLAEFTASGCNWNVATIGWMYTLFFVFLGSA
                     AALWGGWLEHVGPRKAGVVAAVCWAGGMVISAFGIQSHQLWMMIVGSGVIGGIGLGLG
                     YISPVSTLIKWFPDRRGMATGMAIMGFGGGAMIGSPLAAMLMKHFAVGSDGGVMMTFL
                     VMAAIYFVFMLGGAFGYRIPASNWKPEGWTPPAAKADNAMITTHHVHVKRIWGIPQFW
                     LVWLALCMNVSAGIGVIGLASPMLQEIFGGHLIGLDKKFAELDKPQLAQIAAIAAGFA
                     ALLSLFNIGGRFFWASLSDKLGRKMTYFVFFVLGAILYASLPSSAASGSKLAFVGAVC
                     VILSMYGGGFSTVPAYLADLFGTQMVGAIHGRLLTAWSAAGILGPIVVNYMRDYQLGI
                     GIPREQVYNQTMYILVGFLAIGFLCNLLIKPVDPKYFMSAEELAEEKRLAHERAAANE
                     AGTAVAASEGRTSTGALVLAWLAVGLPLAWGVYMTLVNAAKFFH"
     gene            2116..3201
                     /locus_tag="VF453_22060"
     CDS             2116..3201
                     /locus_tag="VF453_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7690408.1"
                     /translation="MHKISIRPQWTISHDHGEDLPPRLIELLVQVAHTGSLSAACQRT
                     GQSYRHAWDTIRHGEAWFGTELLRMERGRGSSLTPLAEKLVWADRRIAARLAPSLDTL
                     ASELEVELQRVLDPPRRVLRVHASHGFAIERLFAMLGEAGVTVERVYTHSQDAAASLH
                     DGTCDLACFQVPLGPLQARGWEHYARWLDPRLHRVVDITDRTQGLMVAAGNPFGIAGL
                     EDLPRRGLRFINRQPGSGTRFLLDCLLAERGIDPARIVGYEHGEFTHAAVAAFIGSGM
                     ADVGFGLERPARQFALDFLPIVEERYFAVCHRDAFERPEVQRAVALLRSPAFRADVDA
                     LPGYRAYRAGAVHELADLLAAAGVAAA"
     gene            complement(3203..3541)
                     /locus_tag="VF453_22065"
     CDS             complement(3203..3541)
                     /locus_tag="VF453_22065"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7690409.1"
                     /translation="MARSYPASDCPVACALDLVGDRWTLLILRDLSLHPARRFQDFVE
                     SLKGCAPNTLSTRLAALERDGLVERRLYEERPPRMEYLLTAKGREAAPVLRALRNWGL
                     KLQAADAGRR"
     gene            3670..4440
                     /locus_tag="VF453_22070"
     CDS             3670..4440
                     /locus_tag="VF453_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7690410.1"
                     /translation="MELQGKTALVTGGNSGIGFATAKLLVAQGARVAITGRDRVKLDE
                     AAAELGERALPIQADLTEPGAAAAVAARVREAFGRLDIVFANAGIGGQTPLGQTTPEA
                     FAQLLHTNLTSVFFTVQEALPLLGEGGAIVLNGSVMRQIGVPGSAAYAATKGGVTAMA
                     KVFAAELAPRGIRVNTVIPGATRTPIWTRGLRAGATLDDTEKALAPLIPLGRLAEPEE
                     VARAVVFLATTPGVTAAEVVVDGGLTGAPSGAPALRRG"
     gene            complement(4447..>4763)
                     /locus_tag="VF453_22075"
     CDS             complement(4447..>4763)
                     /locus_tag="VF453_22075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7690411.1"
                     /translation="ALYHERCAACHGAQGQGAVAGDRTAYVPLAGNRLVLLESPANLV
                     HVIVNGGFAPSTPGNPRPFGMPPAGQSLKDDEIAALATYVRNAWGNAAPVVSAVDVLE
                     AR"
BASE COUNT          712 a         1736 c         1650 g          665 t
ORIGIN      
        1 accgccctgg cgagcagctc gcgctccccg gcggacgccg ccgggcccga gggccgcggc
       61 gccgcgccgg agcggggcat gccccgcagg tcgtcagtgg aagaacttcg cggcattgac
      121 cagcgtcatg tagacgcccc acgcgagcgg caggccgacg gccagccacg cgagcaccag
      181 ggcgcccgtg ctcgtgcgtc cctccgaggc ggccacggcc gtgcccgcct cgttggcggc
      241 ggcgcgctcg tgggcgaggc gcttctcctc ggcgagttcc tcggccgaca tgaagtactt
      301 cggatcgacc ggcttgatca gcaggttgca caggaagccg atcgcgagga agccgaccag
      361 gatgtacatc gtctggttgt agacctgctc gcgcgggata ccgatgccca gctggtagtc
      421 gcgcatgtag ttgacgacga tcgggcccag gatgcccgcc gccgaccagg cggtcagcag
      481 gcgtccgtgg atcgcgccga ccatctgcgt gccgaacagg tcggcgaggt aggccggcac
      541 ggtcgagaag ccgccgccgt acatgctcag gatgacgcag acggcgccga cgaaggccag
      601 cttcgagccg gaggccgcgc tgctgggcag gctggcgtac aggatcgcgc ccagcacgaa
      661 gaagacgaag tacgtcatct tgcggccgag cttgtccgac aggctcgccc agaagaagcg
      721 cccgccgatg ttgaacaggc tcagcagcgc ggcgaagccg gcggcgatcg cggcgatctg
      781 cgcgagctgc ggcttgtcga gctcggcgaa cttcttgtcc agcccgatca ggtggccgcc
      841 gaagatctcc tggagcatcg ggctcgcgag gccgatcacg ccgatgcccg ccgagacgtt
      901 catgcacagc gccagccaca cgagccagaa ctgcgggatg ccccagatgc gcttcacgtg
      961 cacgtggtgg gtcgtgatca tcgcgttgtc ggccttggcg gccggcggcg tccagccttc
     1021 cggcttccag ttcgacgccg ggatccggta gccgaaggcg ccgccgagca tgaacacgaa
     1081 gtagatcgcg gccatgacga ggaaggtcat catgacgccg ccgtcggagc cgaccgcgaa
     1141 gtgcttcatc agcatcgccg cgagcggcga gccgatcatc gcgccgccgc cgaagcccat
     1201 gatggccatg ccggtggcca tgccgcggcg gtcggggaac cacttgatca gcgtcgagac
     1261 gggcgagatg tagcccaggc ccaggccgat gccgccgatc acgccggagc cgacgatcat
     1321 catccacagc tggtggctct ggatgccgaa cgccgagatc accatgccgc ccgcccagca
     1381 cacggccgcg acgacgccgg ccttgcgcgg accgacatgc tcgagccagc cgccccacag
     1441 cgccgcggcg ctgccgagga agacgaagaa cagcgtgtac atccagccga tggtcgcgac
     1501 gttccagttg cagccgctcg cggtgaactc cgcgaggccc tcgaggatgc cgcccttcgg
     1561 gcaggccggc gcgccggcga gcagcttgga cagcggcagc cagaacaccg agaagccgta
     1621 ggccatcccg atgcacaggt ggatggcgag cgccgcgggc ggcaccatcc agcggttgaa
     1681 gcctgcgccc gcgatggtgc gctccttcga caggagcccc ccggatgccg acgcggcggt
     1741 agagccgttc atgacggtag acatcttgat cctttgattg cctcggggca tcgaccgttc
     1801 ggtgtcccaa tcacccacgg ctgcccgact gtaagtatcc cgtgccgatg tttgcaatat
     1861 gcattcggcc gcctaaagag gaaccctaag ctgtcatgcg accgacaggt ctcgtgaacc
     1921 cgccgcccgc tcgacggcac gcgttttcgc gaccggagct tccatacgtt ttgttgcatt
     1981 tttccggtgc cgtccttcgt cgtcccggag gttccttcgt ccgcagcttg cccgcatcga
     2041 gtgacgtcgt gccgacacgc ggcgcctctt tgttgcaaga tgcgtccgac cgccccgcgt
     2101 cttccgccgc agaccatgca caagatctcg atccgcccgc aatggacgat ctcgcacgac
     2161 cacggcgagg atctgccgcc gcgcctgatc gagctgctcg tgcaggtcgc gcacaccggc
     2221 agcctgtcgg cggcatgcca gcgcaccggg cagtcgtacc gccacgcctg ggacacgatc
     2281 cgccacggcg aggcgtggtt cggcaccgag ctgctgcgca tggagcgcgg ccgcggctcg
     2341 agcctgacgc cgctggccga gaagctcgtg tgggccgacc gccgcatcgc cgcgcggctc
     2401 gcgccctcgc tcgacacgct ggcctcggag ctcgaggtgg agctgcagcg cgtgctcgat
     2461 ccgccgcggc gcgtgctgcg cgtgcatgcg agccacggct tcgcgatcga gcgcctgttc
     2521 gcgatgctgg gggaggcggg cgtgaccgtc gagcgcgtgt acacgcatag ccaggacgcc
     2581 gccgcctccc tgcatgacgg cacctgcgac ctcgcgtgct tccaggtccc gctcggaccg
     2641 ctgcaggcgc ggggctggga gcactacgcg cgctggctcg atccgcgcct gcaccgcgtc
     2701 gtcgacatca ccgaccgcac gcagggcctg atggtcgcgg cgggcaaccc gttcggcatc
     2761 gccggcctgg aggacctgcc ccgccgcggg ctgcgcttca tcaaccgcca gccgggctcg
     2821 ggcacgcgct tcctgctcga ctgcctgctc gccgagcgcg gcatcgaccc ggcgcgcatc
     2881 gtcggctacg agcacggcga gttcacgcac gcggccgtcg cggcgttcat cggcagcggc
     2941 atggccgacg tcggcttcgg cctcgagcgc ccggcgcgcc aattcgcgct cgacttcctg
     3001 ccgatcgtcg aggaacgcta cttcgcggtc tgccaccgcg acgcgttcga gcgccccgag
     3061 gtgcagcgag cggtcgcgct gctgcgctcg cccgcgttcc gtgcggacgt cgacgccctg
     3121 cccggctatc gcgcctaccg cgccggcgca gtgcacgagc tcgccgacct gctcgcggcc
     3181 gcgggcgtcg ccgcagcctg agtcagcgcc gccccgcgtc cgcggcctgc agcttcaggc
     3241 cccagttgcg cagcgcccgg agcacgggcg ccgcctcgcg gcccttggcc gtcagcaggt
     3301 actccatgcg cggcggccgc tcctcgtaga gccggcgctc gacgaggccg tcgcgctcga
     3361 gcgcggccag ccgcgtcgac agcgtgttgg gcgcgcaacc cttgagcgac tcgacgaagt
     3421 cctggaagcg ccgcgccgga tgcagcgaca ggtcgcgcag gatcagcagc gtccagcgat
     3481 cgccgacgag gtcgagcgcg caggcgacgg gacagtccga ggcggggtac gagcgggcca
     3541 tggcgcgaat catacgcgcg tcccttgttg acggatagtt acttcatatt ttatagtcac
     3601 ttccagatcg atatcgacaa cctcgatccg gcccgcacac acgtccactc cctttcgaag
     3661 gaacgcatca tggaactcca aggcaagacc gccctcgtca ccggcggcaa cagcggcatc
     3721 ggcttcgcaa cggcgaagct gctcgtcgcg cagggcgcgc gcgtcgcgat caccggccgc
     3781 gaccgcgtga agctcgacga ggccgccgcc gaactgggcg agcgcgcgct gccgatccag
     3841 gccgacctca ccgagcccgg cgcggcggcc gcggtcgccg cgcgggtgcg cgaggccttc
     3901 ggacgcctgg acatcgtctt cgccaacgca ggcatcggcg gccagacgcc gctcgggcag
     3961 acgacgcccg aggccttcgc gcagctgctg cacaccaacc tcacgtcggt cttcttcacc
     4021 gtgcaggagg cgctgccgct gctcggcgaa ggcggcgcga tcgtcctcaa cggctcggtg
     4081 atgcggcaga tcggcgtccc cggctcggcg gcctacgccg cgaccaaggg cggcgtgacg
     4141 gcgatggcca aggtgttcgc ggcggagctc gcgccgcgcg gcatccgcgt caacaccgtc
     4201 atccccggcg cgacgcgcac gccgatctgg acccgcggcc tgcgcgccgg cgcgacgctg
     4261 gacgacaccg agaaggcgct cgccccgctg atcccgctgg gccggctcgc cgagcccgag
     4321 gaggtcgcgc gcgccgtcgt cttcctcgcg accacgcccg gcgtcacggc ggcggaggtg
     4381 gtggtcgacg gcggcctcac cggcgcaccg agcggggcgc cggccctgcg ccgcggctga
     4441 gtgccgtcag cgcgcctcga gcacgtcgac cgcgctgacc accggcgccg cgttgcccca
     4501 ggcgttgcgc acgtaggtcg ccagcgcggc gatctcgtcg tccttgagcg actgccccgc
     4561 cggcggcatg ccgaagggcc gcgggttgcc gggcgtcgac ggcgcgaagc cgccgttgac
     4621 gatgacgtgc acgagattgg ccggcgactc cagcagcacg agccggttgc ccgccagcgg
     4681 cacgtaggcc gtgcggtcgc ctgccacggc gccctggccc tgggcgccgt ggcaggcggc
     4741 acagcgctcg tggtacagcg cgg
//
LOCUS       DASVLN010000940         8098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_160870, whole genome shotgun sequence.
ACCESSION   DASVLN010000940 DASVLN010000000
VERSION     DASVLN010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160870"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4048
                     /locus_tag="VF453_22080"
     CDS             <1..4048
                     /locus_tag="VF453_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX7690412.1"
                     /translation="RAVSALALVHQRFSTNTFPEWPLAHPYRMVAHNGEINTVKGNFN
                     WMRAREGVMKSPVLGEDLRKLYPISFEGQSDTATFDNTLELLTMAGYPLAHAAMMMIP
                     EAWEQHTVMDARRRAFYEYHAAMMEPWDGPAAMVFTDGKQIGATLDRNGLRPARYIVT
                     EDDLVVMASESGVLAIPENKIVKKWRLQPGKMFLIDLEQGRIVDDEELKNQFASAKPY
                     RQWIDNVRVKLDEVPVTASGAPTSDETPLLDRQQAFGYTQEDVKFLLSPMATLGEEGI
                     GSMGNDSPLAVLSDKNKPLYNYFKQLFAQVTNPPIDPIREAIVMSLNSFIGPKPNLLD
                     INAVNPQMRLEVAQPVLDFDDMARLRAIEKHTFGKFKPHEIDIVYPVDWGREGVEAKL
                     ASLCAEAVDAIRGGHNILILTDRRVSPGLIAIPALLALSAIHQHLVREGLRTTCGLVV
                     ETGSAREVHHFAVLAGYGAEAVHPYLALETLAAMHAELPGELSAEKAIYNYIKAVGKG
                     LSKIMSKMGVSTYMSYCGAQLFEAIGLDTDVTDKYFRGTASQVGGIGVFEIAEEALRM
                     HRAAFGDDPLLAEMLDAGGEYAWRTRGEEHMWTPDAIAKLQHSARSGRFDTYKEYAQI
                     INDQSRRHMTLRGLFEFRIDPAKAIPLDEVEPAKEIVKRFATGAMSLGSISTEAHSTL
                     AIAMNRIGGKSNTGEGGEDPARYRNELKGIPIVQGAKLSEVLGEGVIASDLELQAGDS
                     LRSRIKQVASGRFGVTTEYLVSADQIQIKMAQGAKPGEGGQLPGGKVSEYIGRLRHSV
                     PGVGLISPPPHHDIYSIEDLAQLIHDLKNVSPKSDISVKLVSEVGVGTIAAGVAKCKA
                     DHLVIAGHDGGTGASPWSSIKHAGTPWELGLAETQQTLVLNQLRGRVRVQADGQMKTG
                     RDVVIGALLGADEFGFATAPLVAEGCIMMRKCHLNTCPVGVATQDPVLRAKFSGKPEH
                     VVNFFFFIAEEARQIMAQLGIRRFEDLIGRTDLLDTRKGIAHWKARGLDFARVFAQPA
                     MPANVSRKHEDVQDHGLDKALDRKLIERSLPAIQRGEKVQFMEDARNLNRSVGAMLSG
                     ELVRHRPEGLPDHTLFIQMEGTGGQSFGAFLASGITLYLIGDANDYTGKGLSGGRVVV
                     RPSIDFRGDATKNIIAGNTALYGATSGEAFFRGVAGERFAVRLSGASTVVEGTGDHGC
                     EYMTGGTVVVLGKTGRNFAAGMSGGVAYVYDEDGQFARRCNTAMVALERIAPADEQAA
                     AGDPSSWHRGEADETLLKQMIESHHKWTGSLRAREILDHWADSRARFVKVFPHEYKRA
                     LGEMSAKKEAGATIAKAKSSKSATVPAK"
     gene            4127..5593
                     /locus_tag="VF453_22085"
     CDS             4127..5593
                     /locus_tag="VF453_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX7690413.1"
                     /translation="MGKVTGFMEFERLEEGYAPVPQRVKHYKEFVIGLKVDEAKQQAA
                     RCMDCGTPFCNSGCPVNNVIPDFNDLVYTNHWRHAATVLHSTNNFPEFTGRICPAPCE
                     AACTLNINDDAVGIKSIEHAIIDRAWEEGWVVPQPAAAKTGRKVAVVGSGPAGLAAAQ
                     QLARAGHEVTVFEKNDRVGGLLRYGIPDFKMEKSHIDRRVAQMEAEGVTFRTGVVVGD
                     WPKGTKITNHAKESVSAEQLKAEFDAVLLAAGAEVSRDLPVPGRDLEGVHFAMEFLPQ
                     QNKVNAGDKLKGQLTAAGKNVIVIGGGDTGSDCVGTSNRHGAKSVTQFELMPMPPEHE
                     DKPLVWPYWPTKLRTSSSHEEGCSREFAIATKEFLGDRGKLTGLKTVQVEFKDGRLAE
                     VPGTEKTWPAELVLLAMGFTQPAASLLQSFGVDADARGNAKAGTDERSGYRTNVDKVF
                     AAGDVRRGQSLVVWAIREGRQAARAIDAALMGISDLPR"
     gene            5953..6762
                     /locus_tag="VF453_22090"
     CDS             5953..6762
                     /locus_tag="VF453_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7690414.1"
                     /translation="MNSDPLIQIDHVTFGYDANRTILNDVSLQFGRGKVTAILGGSGC
                     GKTTLLRLIGGVYKPNKGRIVFDGTEVDTRDRDKLYAMRRRLGMLFQFGALFTDLTVF
                     ENVAFPLREHMALDESMIRDIVLMKLNAVGLRGAAGLRISEVSGGMARRIALARAVAL
                     DPEVIMYDEPFAGLDPISMGVTANLIRRLNDATGATSLVVSHDVQECFAIADYAYLMQ
                     PGGRVVAHGLPQELNASEDPEVRQFIRGEPDGPVKFHYPAPPLAEDLGVPQ"
     gene            6759..7541
                     /gene="mlaE"
                     /locus_tag="VF453_22095"
     CDS             6759..7541
                     /gene="mlaE"
                     /locus_tag="VF453_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEX7690415.1"
                     /translation="MIAIASSLGRVTLRAFTALGHAAFFFVDLLRYLPGAFRRFGLVV
                     AQIYAIGYRSLTIILASGAAVGCVLALQMYYALVPYGASESLGLVVNLSLVRELGPVV
                     TALLFAGRAGTALTAEIGLMKAGEQLSAMELMAVDPRSRVLAPRLLAGMLSMPVLAIL
                     YSAVGIIGAWVIAVQLIGVDPGNFWSIMQAKVDVVRDIGNGIVKSAVFGVICTGVALY
                     QGYETQATPEGVAYATTRTVVFSSLSVLAMDFILTAIMFSTP"
     gene            7577..>8098
                     /gene="mlaD"
                     /locus_tag="VF453_22100"
     CDS             7577..>8098
                     /gene="mlaD"
                     /locus_tag="VF453_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX7690416.1"
                     /translation="MAKKSTETLVGVFVLLGIVALVFLALKAANLGSFSSAKTYRVSA
                     RFDNIGGLKKGAPVRSAGVSVGHVTSITLDKNTYQGLVTMEIKDGIDFPVDSSAKILT
                     AGLLGDQYIGIEPGSDDKNLKGGDVIKQTQSAVVLENLIGQLIFNKAADAGTSTAPAP
                     ASSGTALAAPAAPA"
BASE COUNT         1355 a         2806 c         2774 g         1163 t
ORIGIN      
        1 gcgcgccgtc tccgcgctcg cgctcgtgca ccagcgcttc tcgaccaaca ccttccccga
       61 gtggccgctc gcccacccgt accgcatggt cgcccacaac ggcgagatca acacggtcaa
      121 gggcaacttc aactggatgc gcgcccgcga gggcgtgatg aagtcgcccg tgctgggcga
      181 ggacctgcgc aagctctatc cgatcagctt cgagggccag tccgacaccg cgaccttcga
      241 caacacgctc gagctgctga cgatggcggg ctacccgctc gcgcatgcgg cgatgatgat
      301 gatccccgag gcgtgggagc agcacaccgt gatggatgcg cggcgccgcg cgttctatga
      361 ataccacgcc gcgatgatgg agccgtggga cggccccgcc gcgatggtgt tcaccgacgg
      421 caagcagatc ggcgccaccc tcgaccgcaa cggcctgcgc ccggcgcgct acatcgtcac
      481 cgaggacgac ctcgtcgtga tggcgtcgga atccggcgtg ctcgcgatcc ccgagaacaa
      541 gatcgtcaag aagtggcgcc tgcagccggg caagatgttc ctgatcgacc tcgagcaggg
      601 ccgcatcgtc gacgacgagg agctgaagaa ccagttcgcc tcggccaagc cgtaccgcca
      661 gtggatcgac aacgtgcggg tcaagctcga cgaggtgccc gtcaccgcga gcggcgcgcc
      721 gacctcggac gagacgccgc tgctcgatcg ccagcaggcc ttcggctaca cgcaggagga
      781 cgtcaagttc ctgctcagcc cgatggccac gctgggcgag gagggcatcg gctcgatggg
      841 caacgactcg ccgctggccg tcctgagcga caagaacaag ccgctgtaca actacttcaa
      901 gcagctgttc gcgcaggtca cgaacccgcc gatcgacccc atccgcgagg cgatcgtgat
      961 gtcgttgaac tcgttcatcg gaccgaagcc caacctgctc gacatcaacg cggtcaatcc
     1021 gcagatgcgg ctggaggtgg cgcagccggt gctcgatttc gacgacatgg cgcgcctgcg
     1081 tgcgatcgag aagcacacct tcggcaagtt caagccgcat gagatcgaca tcgtctaccc
     1141 ggtcgactgg ggccgcgaag gcgtcgaggc caagctcgcc tcgctgtgcg cggaagccgt
     1201 cgacgcgatc cgcggcggcc acaacatcct catcctcacg gaccgccgcg tgagcccggg
     1261 gctgatcgcg atccccgcgc tgctcgcgct gtcggcgatc caccagcacc tggtgcgcga
     1321 gggcctgcgc acgacctgcg gcctggtcgt cgagacgggc tccgcgcgcg aggtgcatca
     1381 cttcgccgtg ctcgcgggct acggcgccga ggccgtgcac ccctacctcg cgctcgagac
     1441 gctggccgcg atgcacgcgg agctgccggg cgagctttcg gccgagaagg ccatctacaa
     1501 ctacatcaag gcggtcggca agggcttgtc gaagatcatg tcgaagatgg gcgtcagcac
     1561 gtacatgtcg tactgcggcg cgcagctgtt cgaggcgatc ggcctggaca ccgacgtgac
     1621 ggacaagtat ttccgcggca cggcctcgca ggtcggcggc atcggcgtgt tcgagatcgc
     1681 cgaggaagcg ctgcgcatgc accgcgcggc cttcggcgac gacccgctgc tggccgagat
     1741 gctggatgcc ggcggggagt acgcctggcg cacccgtggc gaggagcaca tgtggacgcc
     1801 ggacgcgatc gccaagctgc agcacagcgc gcgctcgggc aggttcgaca cgtacaagga
     1861 atacgcgcag atcatcaacg accagagccg ccgccacatg acgctgcgcg gcctgttcga
     1921 gttccgcatc gaccccgcga aggcgatccc gctggacgag gtcgagccgg ccaaggagat
     1981 cgtcaagcgc ttcgccacgg gcgcgatgtc gctcggctcg atctcgaccg aggcgcactc
     2041 gacgctcgcg atcgcgatga accgcatcgg cggcaagagc aacaccggcg agggcggcga
     2101 ggatccggcg cgctaccgca acgagctgaa gggcatcccg atcgtccagg gcgcgaagct
     2161 ctcggaggtg ctgggcgagg gcgtcatcgc gtccgacctc gagctgcagg ccggcgacag
     2221 cctgcgcagc cgcatcaagc aggtcgcgtc gggccgcttc ggcgtgacga ccgagtacct
     2281 ggtgtcggcc gaccagatcc agatcaagat ggcccagggc gcgaagccgg gcgagggcgg
     2341 ccaactgccg ggcggcaagg tctccgagta catcggccgc ctgcgccact cggtgccagg
     2401 cgtcggcctg atctcgccgc cgccgcacca cgacatctac tcgatcgagg acctggcgca
     2461 gctgatccac gacctgaaga acgtcagccc gaagtcggac atcagcgtga agctggtgtc
     2521 ggaggtcggc gtcggcacga tcgccgcagg cgtggccaag tgcaaggccg accacctcgt
     2581 catcgccggc catgacggcg gcaccggtgc gtcgccgtgg tcgtcgatca agcacgccgg
     2641 cacgccgtgg gagctgggcc tggcggagac gcagcagacg ctcgtgctca accagctgcg
     2701 cggccgcgtc cgggtgcagg ccgacggcca gatgaagacc ggtcgcgacg tcgtcatcgg
     2761 cgcgctgctg ggcgccgatg aattcggctt cgcgaccgcg ccgctggtcg ccgagggctg
     2821 catcatgatg cgcaagtgcc acctcaacac gtgcccggtc ggcgtggcca cgcaggatcc
     2881 ggtgctgcgc gcgaagttct cgggcaagcc cgagcacgtc gtcaacttct tcttcttcat
     2941 cgccgaggaa gcgcgccaga tcatggcgca gctgggcatc cgccggttcg aggacctcat
     3001 cggccgcacc gacctgctcg atacccgcaa gggcatcgcc cactggaagg cgcgcggcct
     3061 ggacttcgcc cgcgtgttcg cgcagcccgc gatgccggcc aacgtctcgc gcaagcacga
     3121 ggacgtgcag gaccacggcc tggacaaggc cctggaccgc aagctcatcg agcgctcgct
     3181 gcccgccatc cagcgcggcg agaaggtgca gttcatggag gacgcgcgca acctgaaccg
     3241 ctccgtcggc gcgatgctct cgggcgagct ggtgcgccat cgcccggagg gcctgcccga
     3301 ccacacgctg ttcatccaga tggaaggcac gggcggccag agcttcggcg cgttcctcgc
     3361 atccggcatc acgctctacc tgatcggcga cgccaacgac tacaccggca agggcctgtc
     3421 cggcggccgc gtggtggtgc ggccgtcgat cgacttccgc ggcgacgcca cgaagaacat
     3481 catcgccggc aacaccgcgc tgtacggcgc gaccagcggc gaggccttct tccgcggcgt
     3541 cgcgggcgag cgcttcgcgg tgcggctgtc cggcgcgtcg accgtcgtcg agggcaccgg
     3601 cgaccacggc tgcgagtaca tgaccggcgg caccgtcgtc gtcctcggca agaccgggcg
     3661 caacttcgcg gccggcatgt cggggggcgt ggcctacgtc tacgacgagg atggccagtt
     3721 cgccaggcgc tgcaacaccg cgatggtggc gctcgagcgg atcgcgcccg ccgacgagca
     3781 ggcggcggcc ggcgacccga gctcctggca ccgcggcgag gccgacgaga cgctgctcaa
     3841 gcagatgatc gagtcgcacc acaagtggac gggcagcctg cgcgcgcgcg agatcctcga
     3901 tcactgggcc gactcacgcg cccgcttcgt gaaggtattc ccgcacgagt acaagcgcgc
     3961 gctcggcgag atgtcggcca agaaggaggc cggcgcgacg atcgcgaagg cgaagtcgtc
     4021 gaagtcggcc accgtgcccg cgaagtaagg cgctaaccag aacatgcgtc gccgcgcgct
     4081 tccagcgccg cggcgacgcc tcccgaacag gcatcaggat cgtcaaatgg gaaaagtcac
     4141 cggcttcatg gagttcgagc gtctcgagga gggttatgcg cccgtgccgc agcgcgtgaa
     4201 gcactacaag gaattcgtga tcggcctgaa ggtcgacgag gcgaagcagc aggcggcgcg
     4261 ctgcatggac tgcggcacgc cgttctgcaa cagcggctgt ccggtcaaca acgtgatccc
     4321 ggacttcaac gacctcgtct acacgaacca ttggcggcac gcggcgaccg tcctgcactc
     4381 gacgaacaac ttccccgagt tcaccggccg gatctgcccc gcgccgtgcg aggcggcgtg
     4441 cacgctcaac atcaatgacg acgcggtcgg catcaagtcg atcgagcacg cgatcatcga
     4501 ccgcgcgtgg gaggagggct gggtcgtgcc gcagccggcg gccgcgaaga cgggtcgcaa
     4561 ggtcgccgtc gtgggatccg gccccgcggg cctggccgcg gcgcaacagc tcgcgcgcgc
     4621 cggccacgag gtgacggtgt tcgagaagaa cgaccgcgtc ggcggcctgc tgcgctatgg
     4681 catccccgac ttcaagatgg agaagtcgca catcgaccgc cgcgtcgcgc agatggaggc
     4741 cgaaggcgtg acctttcgca ccggcgtggt cgtcggcgac tggcccaagg gcacgaagat
     4801 cacgaaccac gccaaggagt cggtgagcgc cgagcagctg aaggcggagt tcgacgccgt
     4861 gctgctggcc gcgggcgcgg aggtctcgcg cgacctgccg gtgccggggc gcgacctcga
     4921 gggcgtgcac ttcgcgatgg agttcctgcc gcagcagaac aaggtcaacg ccggcgacaa
     4981 gctcaagggc cagctgacgg ccgcgggcaa gaacgtgatc gtgatcggcg gcggcgacac
     5041 gggcagcgac tgcgtgggca ccagcaaccg ccatggcgcg aagagcgtca cccagttcga
     5101 gctgatgccc atgccccccg agcacgagga caagccgctc gtctggccgt actggccgac
     5161 caagctgcgc acctcgtcgt cgcacgagga gggctgctcg cgcgagttcg cgatcgccac
     5221 gaaggagttc ctcggcgaca ggggcaagct caccggcctg aagaccgtgc aggtcgagtt
     5281 caaggatggc cgcctggccg aagtgccggg caccgagaag acctggcccg ccgagctggt
     5341 gctgctggcg atgggcttca cccagccggc ggcgtcgctg ctgcagtcgt tcggcgtcga
     5401 cgccgatgcg cgcggcaacg ccaaggccgg caccgacgag cgcagcggct accgcacgaa
     5461 cgtcgacaag gtgttcgccg ccggcgacgt gcggcggggc cagtcgctgg tcgtgtgggc
     5521 gatccgcgag ggccgccagg ccgcgcgcgc gatcgatgcc gcgctgatgg gcatcagcga
     5581 cctgccgcgc tgaactcccc cgagtcggcc cggccgtcga ggccggcggc cggcggccgg
     5641 cgccggcctt ggccgcgcgc catacaccca cgctcgcgcc agggacttgc gtcggcgcat
     5701 ccggcccctt gccggcggcc ggaaacgttg cgcgcgcgcg acgacgccgc agctcgtcgg
     5761 cgtttccagg ggcttcggcc gtgacggcga gcgggggacg caaagcttcg cacacaatgc
     5821 ggacacggga tatcccgtat cctgtcgagt tcagacaggt aaccgacagc gttcgcgccg
     5881 gcgtgtcgac gacgacccgc cgcgcggcgc ccccatgagc gacgccgcgc tggcgcggcg
     5941 ctgagagccg gcttgaactc cgatcccctc atccaaatcg accacgtcac gttcggctac
     6001 gacgcgaacc gcacgatcct gaacgacgtg tcgctccagt tcggccgcgg caaggtgacg
     6061 gccatcctgg gcggctcggg ctgcggcaag acgacgctgc tgcggttgat cggcggcgtc
     6121 tacaagccca acaaggggcg catcgtgttc gacggcacgg aggtcgacac ccgcgatcgc
     6181 gacaagctct acgccatgcg ccgccgcctg ggcatgctgt tccagttcgg cgcgctgttc
     6241 accgacctca cggtcttcga gaacgtcgcc tttccgttgc gcgagcacat ggccctggac
     6301 gaatcgatga tccgcgacat cgtgctcatg aagctcaacg cggtggggct gcggggcgcc
     6361 gccggactgc gcatctccga ggtctcgggc ggcatggcgc ggcggatcgc cctcgcgcga
     6421 gccgtcgcgc tggatcccga ggtcatcatg tacgacgagc ccttcgcggg gctggacccg
     6481 atctcgatgg gcgtcaccgc gaacctgatc cggcggctga acgacgccac cggtgcgacg
     6541 tcgctggtgg tctcccacga cgtgcaggag tgcttcgcga tcgccgacta cgcctacctg
     6601 atgcaaccgg gcggccgcgt cgtcgcccac ggcctgccgc aggagctcaa cgcctccgag
     6661 gatccggagg tgcgccagtt catccgcggc gagcccgacg gcccggtcaa gttccattac
     6721 ccggccccgc ccctggccga ggacctggga gtgccgcagt gatcgcgatc gccagctccc
     6781 tcggccgcgt cacgctgcgc gcgttcaccg cgctgggcca cgccgccttc ttcttcgtgg
     6841 acctgctgcg ctacctgccc ggcgcgttcc ggcggttcgg cctggtcgtc gcgcagatct
     6901 acgccatcgg ctaccgctcg ctgaccatca tcctcgcctc gggcgcggcg gtgggctgcg
     6961 tgctcgcgct gcagatgtac tacgccctcg tgccgtacgg tgcttccgag tcgctgggcc
     7021 tggtggtcaa cctctcgctc gtgcgcgagc tcgggccggt ggtcacggcg ctgctgttcg
     7081 ccggccgcgc aggaacggcg ctcaccgcgg agatcggcct gatgaaggcg ggcgagcagc
     7141 tgtcggcgat ggagctgatg gcggtcgacc cgcgcagccg cgtgctcgcg ccgcgcctgc
     7201 ttgccggcat gctctcgatg ccggtgctcg cgatcctcta ctcggcggtc ggcatcatcg
     7261 gcgcgtgggt gatcgcggtg cagctgatcg gcgtggaccc gggcaacttc tggtcgatca
     7321 tgcaggccaa ggtggacgtc gtgcgcgaca tcggcaacgg catcgtgaag tcggcggtgt
     7381 tcggcgtcat ctgcaccggc gtcgcgctct atcagggcta cgagacccag gcgacccccg
     7441 agggcgtcgc ctacgccacc acgcgcaccg tggtgttctc ctcactgtcg gtgctggcga
     7501 tggacttcat cctcaccgcc atcatgttct cgacgccgtg accgtcgcgg cgctaaaaga
     7561 aaaccggatc tggaaaatgg ccaagaaaag taccgagacg ctcgtgggcg tgttcgtcct
     7621 gctggggatc gtcgccctgg tcttcctcgc cctcaaggcg gccaacctgg gcagcttcag
     7681 cagcgcgaag acctatcggg tgagcgcccg cttcgacaac atcggcggcc tcaagaaggg
     7741 ggccccggtg cgcagcgccg gggtgtccgt cggtcacgtc acgtcgatca cgctggacaa
     7801 gaacacgtac cagggactgg tgacgatgga gatcaaggac ggcatcgact tcccggtcga
     7861 ctcctccgcc aagatcctga ccgcgggcct gctgggcgac cagtacatcg gcatcgagcc
     7921 cggctcggac gacaagaacc tgaagggcgg cgacgtgatc aagcagacgc agtcggccgt
     7981 cgtgctcgag aacctgatcg gccagctgat cttcaacaag gccgccgacg ccgggacgag
     8041 taccgccccc gcgcccgcat cctccggcac ggcgcttgct gccccggccg cgcccgcc
//
LOCUS       DASVLN010000941         6032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_210713, whole genome shotgun sequence.
ACCESSION   DASVLN010000941 DASVLN010000000
VERSION     DASVLN010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210713"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2017
                     /locus_tag="VF453_22105"
     CDS             <1..2017
                     /locus_tag="VF453_22105"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7690417.1"
                     /translation="VATGRRARDGLLVALDAPEGGRQQLWRLEPGTGRLELVLANDAD
                     DADEAWIADDALVPRFARRTAADGRVQWHRVVDGRTTDEVIATIDPGDAFATHPLGLA
                     ADGRTLYWLAARGRDTAALLAQDVVTGGWRTLAADPRADIETVLLDPGSGRPQAWVRP
                     AAREAIVALEPAFARDLAVLRRAAGPAGRIEITSRSDDDRRWVVAIDRVVEPLRTMLY
                     DRDRRRLVPFYVGRAALADAPLVPMRPLTIRARDGLPLKAYLSRPAGEGAAPLVLLVH
                     GGPWERDAYGYNSVHQWLANRGYAVLSVDFRGSTGFGRRFLDAGDGQWGRAMEDDLLD
                     AVAWGLAHGVARPGRIAIMGASYGGYAALAGLAFSPDAFACGIDLAGPVDLPAMLDSI
                     PASWTSYRAQMLRRVGDPSTADGRARLEAVSPLGAAGRIARPLLVVAGANDPQVPVEQ
                     SRRLVAALAARGAPVTALVFPREGHGLVRTGDDLAFRAVVENFLAPCLGGRAQAIGDA
                     LRASDVEVPSDRAHVPGLAEAVAAREAGEGEARRQPVGLATGSTEEGGSVAPSAVDPV
                     ARHAAANVTSDPAGNAPAIQGRRAIVSRGGATHGSGVIRAPDSPSTSASSPEPVAPQV
                     RDSRAGNVHSMEIRLRAAQPVDGCVSNHARASATSAAMPSCSASRPP"
     gene            complement(1927..2937)
                     /locus_tag="VF453_22110"
     CDS             complement(1927..2937)
                     /locus_tag="VF453_22110"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX7690418.1"
                     /translation="MQIHRGLRSPATLALARHGTALTIGNFDGVHRGHQAMLALLRSE
                     AQHRGVPSCVLTFEPHPRDFFARRAGKPELAPARISPLRDQLRELERCGVDHVVLLRF
                     DAHLASLAPEAFVDDVLVRGLGVRYLLVGDDFRFGARRAGDYAMLDALGSERGFDVAR
                     MMSYEVHGIRVSSSAVRESLAAGDLPRAASLLGRPYAISGRVAHGRKLGRALGETTAG
                     AGDGFRTLNLRLPDARPAAMGIFVSRVHGLAPHPLPAVSSLGVRPTVEKDGQPLLETH
                     CLDWPAELGRDGAYGRLVQVELVKWLHEERRYGGLEALHDGIAADVADARAWFETHPS
                     TG"
     gene            complement(3236..5185)
                     /locus_tag="VF453_22115"
     CDS             complement(3236..5185)
                     /locus_tag="VF453_22115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7690419.1"
                     /translation="MSTTPMSTSPDARATPAAIERQIEFERVSTLYAVAPPTLIGGIA
                     YALLVAVMLWSLVPHGVLVAWVAFKVVLGTLRLVDGRVFARQRHRSLRMVHWKNRFFA
                     ASLLDACSWGLMTVLFMPTADPVINGVMIAGLVGVASAGVLALAAHRVASGASLALVL
                     GPLVELQAIAPTALERFTAATLFVYGAVLFVEAQNSHRRFSETMRLRFENAAIAEERH
                     RALVYAEHSNAAKTRFLASVSHELRTPLNGIIGMTQLVAVEPLSPLQRQRMEVLRHSA
                     EHLVTVIGDLLDLSRIEFDRIELNPQPALLAQTVRDVTDLLQPVAEERGLTFEVVFDL
                     GLPAAALIDASRVKQVLHNLIGNALKFTPSGEVGLSVGMSDGLLRFRVTDTGEGIPPE
                     AIERIFDAFEQGPSTTAPMRSGAGLGLTISRRLARAMGGDVTCRSAPGKGTTFEFTIA
                     FRPTPRTPSPRPSAAAEITAAATPRRPGDLRPGHVLVVEDNEVNALVVRGMLEQMGVH
                     AELAVNGQKALAAMRLSDFDLVLMDCQMPVVDGWEATRIWRSHEERTRQERRTPIVAL
                     TASAAAGERERCLEAGMDDYLSKPFTRSALANIIDRYLPTTAPPQAGARSAAGAPLVN
                     AVAPPEVESEAPTLDDIPGTPATDN"
     gene            complement(5403..>6032)
                     /locus_tag="VF453_22120"
     CDS             complement(5403..>6032)
                     /locus_tag="VF453_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7690420.1"
                     /translation="GAADDPAGIALLLVEREAAGVATRGYALQDGSRAAEVAFDATPA
                     TLLVPAGEGFAALEHAIDVGIAALAAEGVGVMDRLFELTVDYLNTRKQFGVPIGTFQA
                     LRHRIADMKMQLELARSMSYFATLKLAEEPAARRRAIAQAKVQLGNSARFLGQQAIQL
                     HGGIGMTDEYVGSHYFKRLTTMEMQFGDTMHHLGEVSARMQETAGVFVG"
BASE COUNT          808 a         2168 c         2237 g          819 t
ORIGIN      
        1 cgtcgcgacg ggtcgcaggg cgcgcgacgg gctgctggtc gccctcgacg cacccgaggg
       61 cggtcggcag cagctgtggc gcctcgagcc gggcacgggc cgcctcgagc tcgtgctggc
      121 caacgacgcg gacgacgccg acgaggcctg gatcgccgac gacgcgctcg tgccgcgctt
      181 cgcacgccgc acggccgcgg acgggcgcgt gcagtggcat cgcgtcgtcg acggccgcac
      241 caccgacgag gtgatcgcca cgatcgaccc cggcgacgcg ttcgccacgc atccgctcgg
      301 cctggccgcc gacggccgca cgctctactg gctggcggcg cgcggacgcg acacggccgc
      361 gctgctcgcg caggatgtcg tcaccggcgg ctggcgcacc ctcgcggccg atccgcgggc
      421 cgacatcgag accgtgctgc tcgaccctgg cagcggccgt ccccaggcgt gggtgcgtcc
      481 ggccgcgcgc gaggctatcg tcgcgctgga gcctgcgttc gcgcgcgatc tcgcggtgct
      541 gcggcgcgca gccgggccgg cggggcgcat cgagatcacc tcgcgctccg acgacgaccg
      601 ccgctgggtc gtggccatcg accgcgtcgt ggagccactg cgcaccatgc tctacgaccg
      661 cgaccggcgc cggctcgtcc cgttctacgt cggccgcgcc gcgctggcgg acgcgccgct
      721 ggtgccgatg cggccgctga cgatccgcgc ccgcgacggc ctgccgctca aggcctacct
      781 gagccggccc gcgggcgagg gggccgcgcc gctggtgctg ctggtgcacg gcggcccatg
      841 ggagcgcgac gcctatggct acaacagcgt ccaccagtgg ctcgccaatc gcggctacgc
      901 cgtgttgtcc gtcgacttcc gcggctcgac gggcttcggc cggcgcttcc tcgacgccgg
      961 tgacggccag tggggccgcg cgatggagga cgacctgctc gacgctgtcg cgtggggcct
     1021 tgcgcatggc gtcgcgcggc cggggcgcat cgcgatcatg ggcgcatcgt acggcggcta
     1081 tgcggcgctg gccggcctgg cgttctcgcc cgatgccttc gcctgcggga tcgacctggc
     1141 cggccccgtc gacctgccgg cgatgctgga ttcgatcccg gcttcgtgga cgtcgtatcg
     1201 tgcgcagatg ctgcgtcgcg tcggcgatcc gtcgaccgcc gatgggcggg cgagactgga
     1261 ggccgtctcg ccgctggggg ccgccggccg catcgcacgg ccgctgctgg tggtcgcagg
     1321 cgcgaacgat ccgcaggtgc ccgtggagca atcgagacga ctcgtggccg cgctggccgc
     1381 gcgcggcgcg ccggtcaccg cgctcgtctt cccccgcgaa ggccacggcc tcgtccgtac
     1441 cggcgacgat ctcgcttttc gcgccgtcgt cgagaacttc ctggctccct gtctgggtgg
     1501 ccgcgcacaa gccatcggcg atgcgctgcg cgcgagcgac gtcgaggtgc catccgatcg
     1561 ggcgcacgtg cccggcctcg cggaggcggt ggcggctcgc gaggccgggg agggcgaggc
     1621 acgccggcaa cccgttggcc tggcgacggg ctcgaccgag gaaggcggca gcgtggcgcc
     1681 gagcgccgtc gatccggtgg cccgccatgc ggcggcgaac gtgacgagcg atccggccgg
     1741 caacgcgccg gcaatccagg ggcgccgggc gatcgtcagc cgcggcggag cgacgcatgg
     1801 ctccggcgtg atacgcgcgc ccgactcgcc ttcgacatcg gcctcgtcgc ccgaaccggt
     1861 ggcgccgcaa gttcgcgaca gccgcgccgg aaacgtccac tcgatggaaa tccgcctgcg
     1921 cgcggctcag cctgtcgacg gatgcgtctc gaaccacgca cgggcatcgg cgacgtcggc
     1981 ggcgatgccg tcgtgcagcg cctcgaggcc gccgtagcgg cgctcttcgt gcagccactt
     2041 caccagctcg acctgcacga ggcggccgta ggcgccatcg cgtccgagct cggcgggcca
     2101 gtcgaggcag tgcgtctcga gcagcggctg gccgtccttc tccaccgtcg gtcgcacgcc
     2161 caggctcgag acggccggca gcggatgcgg cgcgaggccg tgcacgcgcg agacgaagat
     2221 gcccatcgcc gccggtcgcg cgtcgggcag gcgcaggttc agcgtgcgga acccgtcgcc
     2281 ggctcccgcg gtggtctcgc ccagcgcgcg gccgagcttg cgcccgtgcg cgacccggcc
     2341 gctgatggcg taggggcggc ccagcagcga ggcggcgcgc gggaggtcgc cggccgccag
     2401 cgactcgcgc accgcggagc tcgagacgcg gatgccatgc acctcgtagc tcatcattcg
     2461 ggcaacgtcg aagccgcgtt cgctgccgag agcgtcgagc atcgcgtagt cgcccgcacg
     2521 gcgcgctccg aagcggaagt cgtcgcccac gagcaggtag cgcacgccca ggccgcgcac
     2581 caggacgtcg tcgacgaagg cctccggcgc cagcgaggcc aggtgggcgt cgaagcgcag
     2641 cagcacgacg tggtcgaccc cgcagcgctc gagctcgcgc agctggtcgc gcagaggcga
     2701 gatgcgcgcg ggggcgagct cgggtttgcc ggcgcggcgc gcgaagaagt cgcgcgggtg
     2761 cggctcgaac gtgagcacgc aactgggcac gccgcggtgc tgcgcctcgc tgcgcaacag
     2821 agcgagcatg gcctggtgac cgcgatgtac gccgtcgaag ttgccgatgg tcagggccgt
     2881 gccgtgccgt gcgagggcga gcgtcgcggg cgagcggagg ccacggtgga tctgcatggc
     2941 gcgattatcg gctgcgcggc cgcgctcgca ggcgacggcc ccgcgcgacg caagggcagg
     3001 gctggcgaag ttcacggacg gggttgtgcc ggagcgtgtc gacggagccg cgcgctgagc
     3061 tccgcggtgc tcggtgcggg cccgtcgact ggagggcgtg cacgatcgcg gacgacgagg
     3121 agcgaaccag gcctgcccga caagaagtac ttcgtcgccg atgcactgga acttcgtcgg
     3181 ccgctcggtg cgggcgctca cggccggttt gacgagcccg cgacgcacgc cgacgtcagt
     3241 tgtccgtcgc aggcgttccg ggtatgtcgt cgagggttgg ggcttccgac tccacctccg
     3301 gcggcgcgac ggcattcacg agaggcgcgc ccgcggcgct tctcgcaccg gcctgcggcg
     3361 gcgcggtcgt gggcaggtag cgatcgatga tgttggccag cgccgaccgc gtgaagggct
     3421 tgctcaggta gtcgtccatg ccggcctcga ggcagcgctc gcgttcgccc gcggcggcgc
     3481 tggccgtcag tgcgacgatc ggggtacgcc gttcctggcg cgtgcgctcc tcatgcgagc
     3541 gccagatgcg cgtcgcctcc cagccatcga ccaccggcat ctggcagtcc atcagcacga
     3601 ggtcgaagtc cgacagccgc atcgccgcga gcgccttctg gccgttgacc gcgagctccg
     3661 catgcacgcc catctgctcg agcatgccgc gcacgacgag cgcgttgacc tcgttgtcct
     3721 cgaccaccag cacgtggccg ggccgcaggt cgcccgggcg gcgcggagtg gccgcggcgg
     3781 tgatctcggc cgcagccgac gggcgcggac tgggcgtgcg cggcgtcgga cggaaggcga
     3841 tcgtgaactc gaaggtcgtg cccttgcccg gcgccgagcg gcaggtgacg tctcccccca
     3901 tcgcgcgcgc gagccggcgc gagatcgtca ggccgagccc cgcgcccgag cgcatgggcg
     3961 cggtcgtcga cgggccctgc tcgaaggcat cgaagatgcg ctcgatggcc tcgggcggta
     4021 taccctcgcc ggtatctgtc acgcggaagc gcagcagccc gtcgctcatg cccaccgaca
     4081 ggccgacctc gcccgagggc gtgaacttca gcgcgttgcc gatcaggttg tgcagcacct
     4141 gcttcacgcg cgacgcgtct atgagcgccg ccgccggcag cccgaggtcg aacaccacct
     4201 cgaaggtgag cccacgctcc tcggcgacgg gctgcagcag gtcggtgacg tcgcgcacgg
     4261 tctgcgcgag cagcgccggc tgcgggttga gctcgatgcg gtcgaactcg atgcgcgaga
     4321 ggtcgagcag gtcgccgatc acggtgacca ggtgctcggc ggaatggcgc agcacttcca
     4381 tgcgctggcg ctgcagcggc gacagcggct cgaccgccac cagctgcgtc atgccgatga
     4441 tgccgttcag cggtgtgcgc agctcgtggc tgaccgaggc gaggaagcgc gtcttggcgg
     4501 cgttggagtg ctccgcgtag acgagcgccc ggtggcgttc ctcggcgatg gcggcgttct
     4561 cgaagcgcag gcgcatcgtc tcgctgaagc ggcggtggct gttctgcgcc tcgacgaaga
     4621 gcaccgcgcc gtacacgaac agcgtcgccg cggtgaagcg ctccagcgcg gtgggcgcga
     4681 tggcctgcag ttccaccagc ggccccagca ccagggcgag cgacgcgccc gaggccacgc
     4741 ggtgcgccgc cagcgcgagc accccggcag aggccacgcc gaccaggccc gcgatcatca
     4801 cgccgttgat gacgggatcg gcggttggca tgaacagcac cgtcatgagg ccccagctgc
     4861 acgcgtccag caggctggcg gcgaagaacc ggttcttcca atgcaccatg cgcaagctgc
     4921 gatggcgctg gcgcgcgaac acgcggccgt cgaccagccg cagcgtcccc agcaccacct
     4981 tgaaggcgac ccaggcgacg agcaccccgt gcggcaccag cgaccacagc atgacggcca
     5041 cgagcagcgc atacgcgatg ccgccgatca gcgttggcgg cgcgaccgcg tagagcgtgc
     5101 tgacccgctc gaactcgatc tgccgctcga tcgcggcggg cgtggcgcgg gcgtcgggag
     5161 acgtcgacat cggcgtcgtg gacacggcgg agcgatgtac cccctagggt acccgcacct
     5221 ggacgtcgaa ctgcagcgtc atgcggcgca gtctaccgac ttttgctccg tccgcgcggc
     5281 tttgcggatg cgagatcgcg cgagcgcgga gcggcacgcc ggagctcccg gagcgatgca
     5341 tttcccgcag gcttggcgcc ggtcttgtgc gcgaaggcgg ccgcgaccgc ccgccccccg
     5401 gctcagccga cgaagacgcc ggccgtctcc tgcatccggg ccgagacttc gcccaggtgg
     5461 tgcatcgtgt cgccgaactg catctccatc gtcgtcagcc gcttgaagta gtggctgccc
     5521 acgtattcgt ccgtcatgcc gatgccgccg tgcagctgga tcgcctgctg gcccaggaag
     5581 cgcgcgctgt tgcccagctg aaccttggct tgcgcgatcg cgcgccggcg cgcggccggc
     5641 tcctcggcga gcttgagcgt cgcgaagtag ctcatcgagc gcgcgagctc cagctgcatc
     5701 ttcatgtcgg cgatgcgatg gcgcagcgcc tggaaggtgc cgatcggcac gccgaactgc
     5761 ttgcgcgtgt tcaggtagtc gaccgtcagc tcgaacaggc ggtccatcac gccgacgccc
     5821 tcggccgcga gcgccgcgat gccgacgtcg atcgcgtgtt cgagcgccgc gaagccctcc
     5881 ccggcgggga cgagcagcgt cgcgggcgtc gcgtcgaagg cgacctcggc cgcgcgcgag
     5941 ccgtcctgca gcgcgtagcc gcgcgtcgcg acgcccgcgg cttcgcgctc gaccagcagc
     6001 agggcgatgc cggcggggtc gtccgccgcg cc
//
LOCUS       DASVLN010000942         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_320022, whole genome shotgun sequence.
ACCESSION   DASVLN010000942 DASVLN010000000
VERSION     DASVLN010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320022"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VF453_22125"
     CDS             complement(<1..838)
                     /locus_tag="VF453_22125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7690421.1"
                     /translation="MSLLEIRNLSVEFGPPGRAAPAVRGVDLALERGELLGVVGESGS
                     GKSVSMLALMGLVDAPGRVRADAMRFDGHDLLALAPRERRRIVGRDIAMIFQDPTTSL
                     NPSWPVGFQIVETLKAHRPASRAAMRERAVELLRLVEIPDPEARLAAYPHQLSGGMNQ
                     RVMIAMALACHPKLLIADEPTTALDVTIQAQVLDLLVGLQRTQDLGLVLISHDLALLA
                     ERAQRIAVMYAGEIVEEQPVPALFEAPRHPYTAALLAAIPEASRGAGRLPALPGRVPG
                     PEE"
     gene            complement(835..>1588)
                     /locus_tag="VF453_22130"
     CDS             complement(835..>1588)
                     /locus_tag="VF453_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7690422.1"
                     /translation="ASHDPIVQYRDDLLAPPAWSAGGTWRFPLGTDALGRDLLSRLLF
                     GARLSLATGAVAVTLSVLPGVALGLAAAFFPRVGVVVLRLMDVLYAVPSLLLAIAVVA
                     VLGPGLLNATAAIAIVSIPGYVRLVRVAALGEIVKDYVVASRLAGAGTARLMFDTVLP
                     NCLAPVIVTATLGFSDAILTAAGLGFLGLGAQPPMPEWGTMLASARDFIERANWVVTF
                     PGLAILVTVLAVNLVGDGLRDALDPKLKKVGR"
BASE COUNT          234 a          565 c          615 g          174 t
ORIGIN      
        1 gctcctcggg gccgggcacg cggcccggca gcgcgggcag gcgaccggcg ccgcggctgg
       61 cctcggggat ggccgccagc agcgccgccg tgtacggatg gcgcggcgcc tcgaacagcg
      121 cgggcaccgg ctgctcctcg acgatctcgc ccgcgtacat cacggcgatg cgctgcgcgc
      181 gctcggccag cagcgcgagg tcgtgcgaga tcagcaccag gccgagatcc tgcgtgcgct
      241 gcaggccgac cagcaggtcc agcacctggg cctggatcgt cacgtcgagg gcggtcgtcg
      301 gctcgtcggc gatcagcagc ttgggatggc aggcgagcgc catcgcgatc atcacgcgct
      361 ggttcatgcc gcccgagagc tggtgcgggt aggcggcgag ccgcgcctcg gggtcgggga
      421 tctcgaccag ccgcagcagc tccaccgcgc gctcgcgcat cgccgcgcgc gaggccgggc
      481 ggtgcgcctt cagcgtctcg acgatctgga agccgacggg ccagctcggg ttgaggctgg
      541 tcgtgggatc ctggaagatc atcgcgatgt cgcgaccgac gatgcgccgg cgttcgcgcg
      601 gcgcgagcgc gagcaggtcg tggccgtcga agcgcatcgc gtcggcgcgg acgcggccgg
      661 gcgcgtcgac gaggcccatc agcgccagca tgctcacgct cttgcccgag cccgattcgc
      721 ccacgacgcc cagcagctcg ccgcgctcga gcgcgaggtc gacgccgcgc acggccggcg
      781 cggcgcggcc gggcgggccg aactcgacgc tcaggttgcg gatctcgagc aggctcatcg
      841 gcccaccttc ttgagcttcg gatcgagcgc gtcgcgcagc ccgtcgccga cgaggttgac
      901 cgccagcacc gttacgagga tagccaggcc cgggaaggtg acgacccagt tcgcgcgctc
      961 gatgaagtcg cgcgccgacg cgagcatcgt gccccattcg ggcatcggcg gctgcgcgcc
     1021 caggccgagg aagcccaggc cggcggcggt gaggatggcg tccgagaagc cgagcgtggc
     1081 ggtcacgatg acgggcgcga ggcagttggg cagcacggtg tcgaacatca gccgcgccgt
     1141 gcccgccccg gccaggcgcg aggcgacgac gtagtccttg acgatctcgc cgagcgccgc
     1201 gacgcgcacc agccgcacgt agccggggat ggagacgatc gcgatcgcgg ccgtcgcgtt
     1261 cagcaggccg ggcccgagca ccgcgacgac cgcgatcgcc agcagcaggc tgggcaccgc
     1321 gtacagcacg tccatgaggc gcaggacgac cacgccgacg cgcgggaaga acgcggcggc
     1381 gagcccgagc gcgacgcccg gcagcaccga cagcgtcacc gcgacggcgc cggtggcgag
     1441 cgacagccgg gcgccgaaca gcaggcgcga gagcaggtcg cggccgagcg cgtcggtgcc
     1501 cagcgggaag cgccacgtgc cgccggcgct ccaggcgggc ggcgcgagca ggtcgtcgcg
     1561 gtactgcacg atggggtcgt gcgacgcg
//
LOCUS       DASVLN010000943         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_104221, whole genome shotgun sequence.
ACCESSION   DASVLN010000943 DASVLN010000000
VERSION     DASVLN010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104221"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VF453_22135"
     CDS             <1..365
                     /locus_tag="VF453_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690423.1"
                     /translation="DVWLQRFSRRRAKSPWSKVNRQKAEALIKAGAMQPPGLAEVERA
                     KQDGRWEQAYDSARSSTVPDDLAAALAAGQRKVEAWVARHAVPVDFDRPGDADAFANF
                     NTLAELHDAGPAGDASAG"
     gene            complement(401..862)
                     /gene="rnhA"
                     /locus_tag="VF453_22140"
     CDS             complement(401..862)
                     /gene="rnhA"
                     /locus_tag="VF453_22140"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX7690424.1"
                     /translation="MTDTTTEVTIYTDGACKGNPGPGGWGCWLSASGRERELFGGAPQ
                     TTNNRMELTAVIKGLSVLTRRCSVAIYTDSEYVRNGITTWIHGWKKRGWRTADGKPVK
                     NVELWQQLETLAHAHDVQWHWVKGHAGDPGNERADQLANKGVQAVLDGTLS"
     gene            complement(900..1715)
                     /locus_tag="VF453_22145"
     CDS             complement(900..1715)
                     /locus_tag="VF453_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7690425.1"
                     /translation="MSGPIIELGGWLKTPPGQVLLAWEQACIDRAVADVFGYHALQFG
                     LPEIEGLRTNRMPHRWLAGDRPDAPPAEAIAPPCDPALTTTPLAAPVALHCDPEALPF
                     PSSSLDLVVLPHTLEFAQDPHLALSEAARVLMPEGRLVITGFNPASLWALRQRLGRAG
                     NGLRRRDGALFLPGEGEFIGYWRLRDWLRLLSFDIEVGRFGCWRPPWTTEVWLERYRW
                     MEPLGERWWPVLGAVYFLVAVRRVRGMRLVGRVKRARMKASAAPAVATNHRRR"
     gene            1720..2487
                     /gene="gloB"
                     /locus_tag="VF453_22150"
     CDS             1720..2487
                     /gene="gloB"
                     /locus_tag="VF453_22150"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX7690426.1"
                     /translation="MELIALPAFADNLIWMLHDGARALVVDPGTATPVEEALQARGLA
                     LEAILVTHHHADHVGGLATLAPRVRGTIHGPARERMPVAVTPVAEGDAFEVLGLRVQV
                     LDVPGHTAGHVAYVVTQPGRAPWLFCGDTLFSGGCGRLFEGTPAQMHDSLTKLAALPG
                     ETRVCCAHEYTLSNLRFARTVEPHNAALADYELRCRALRAEGAWTLPSTIATERAVNP
                     FLRDREPEVLASALAHGAPSPEPVDVFATLREWKNDF"
     gene            2484..>2684
                     /locus_tag="VF453_22155"
     CDS             2484..>2684
                     /locus_tag="VF453_22155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690427.1"
                     /translation="MNPRHLLPRLARRPVLAGLPIAIALALSACSSVDLSTGAAAPVA
                     DATASAPEVAEAASAAASAPVVA"
BASE COUNT          358 a         1009 c          950 g          367 t
ORIGIN      
        1 aggacgtctg gctgcaacgc ttcagccgcc gtcgtgcgaa gagcccctgg tcgaaggtca
       61 accgccagaa ggcggaggcg ctgatcaaag caggcgccat gcagccgccg gggctcgcag
      121 aggtcgagcg cgcgaagcag gacgggcgct gggagcaggc gtacgactcg gcgcggagct
      181 cgaccgttcc ggacgatctc gccgccgcgc tcgccgccgg ccagcgcaag gtcgaggcct
      241 gggtcgcgcg ccacgccgtg ccggtggact tcgaccgtcc cggcgacgcc gacgcgttcg
      301 cgaacttcaa cacgctcgcc gagctgcacg acgccggccc ggcgggcgac gcatccgccg
      361 gttagggcgc gggcggccgg gggccggggg ccgggcgctc ctagctcaag gtcccgtcga
      421 gcaccgcctg cacgcccttg ttggcgagct ggtcggcgcg ctcgttgccc gggtcgcccg
      481 cgtggccctt gacccagtgc cactgaacgt cgtgcgcatg ggcgagcgtc tcgagctgct
      541 gccagagctc cacgttcttg accggcttgc cgtcggccgt gcgccagccg cgcttcttcc
      601 agccgtggat ccacgtcgtg atgccgttgc gcacgtattc gctgtccgtg tagatcgcaa
      661 cggagcagcg ccgcgtgagc accgacaggc ccttgatcac ggccgtgagc tccatgcggt
      721 tgttcgtggt ctgcggcgcg ccgccgaaca gctcgcgttc gcgcccgctc gccgacagcc
      781 agcagcccca gccgcccggg ccggggttgc ccttgcaggc gccgtcggtg tagatcgtca
      841 cttccgtggt ggtatccgtc atggtgcgcg atggtacccg gccgaggcgg ccggccggct
      901 cagcgccgcc gatggttcgt cgccaccgcc ggcgcggccg acgccttcat gcgcgcacgc
      961 ttcacgcgcc cgaccaggcg catcccgcgg acccgccgca cggccacgag gaagtagacc
     1021 gcgcccagca ctggccacca gcgctcgccg agcggctcca tccagcggta gcgctcgagc
     1081 cagacctccg tcgtccaggg cgggcgccag cagccgaagc gaccgacctc gatgtcgaag
     1141 ctgagcaggc gcagccagtc gcgcagccgc cagtagccga tgaactcgcc ctcgccgggc
     1201 aggaacaggg cgccgtcgcg gcgacgcagg ccgttgcccg cacggccgag ccgctggcgc
     1261 agcgcccaca ggctcgccgg gttgaagccc gtgatcacca gccttccttc gggcatcagc
     1321 acgcgcgcgg cctccgacaa tgcgaggtgc gggtcctgcg cgaactccag cgtgtgcggc
     1381 agcacgacca ggtcgaggct gctggacggg aacggcagcg cctcggggtc gcagtgcagc
     1441 gcgaccggcg cggccaacgg cgtcgtcgtg agcgccgggt cgcaaggcgg cgcgatcgcc
     1501 tcggccggcg gcgcgtcggg acggtcgccg gcgagccagc ggtgcggcat gcggttggtg
     1561 cgcaggccct cgatctcggg caggccgaat tgcaacgcgt ggtaaccgaa aacgtccgcg
     1621 accgcgcgat cgatgcacgc ctgctcccag gcgagcagca cctggccggg aggcgtcttc
     1681 agccacccgc ccaactctat aatcggcccg ctcatctcca tggaactgat cgccctcccc
     1741 gcctttgccg acaacctgat ctggatgctc cacgacggcg cccgcgctct cgtggtggat
     1801 cccggaacgg cgacgcccgt cgaggaagcg ctgcaggccc ggggcctggc actcgaggcc
     1861 attctagtga cccatcacca cgccgatcat gtcggcggcc tcgcgacgct cgcgccgcgc
     1921 gtgcgcggca cgatccacgg acccgcgcgc gagcgcatgc cggtggcggt gacgccggtc
     1981 gccgagggcg acgcgttcga ggtgctgggc ctgcgtgtcc aggtgctcga cgtgcccggc
     2041 cacaccgccg gccacgtggc ctacgtggtc acgcagccgg gccgcgcgcc ctggctgttc
     2101 tgcggcgaca cgctgttctc gggcggctgc gggcgactgt tcgagggcac gcccgcgcag
     2161 atgcacgatt cgcttacgaa gctggccgcg ctgcccggcg agacccgcgt atgctgcgcg
     2221 cacgaataca ccctgtccaa cctgcgcttc gcgcgcacgg tcgaaccgca caatgcggcg
     2281 ctggccgact acgagctgcg ttgccgggcg ctgcgcgccg agggcgcgtg gacgctgccc
     2341 tcgacgatcg cgaccgagcg cgcggtcaac ccgttcctgc gcgaccggga gcccgaggtg
     2401 ctcgcgtccg cgctggccca cggcgcgccg tcgcccgagc ccgtggacgt ctttgccacc
     2461 ctgcgtgaat ggaagaacga cttttgaacc ctcgccacct cctgccgcgc ctcgcgcgcc
     2521 gccccgtcct cgcgggcctt ccgatcgcga tcgcgctggc actcagcgcc tgctcgtcgg
     2581 tcgacctgtc gacgggcgcc gcggcaccgg tggccgatgc gacggcctcg gcgcccgagg
     2641 tcgccgaggc cgcgtcggcc gccgccagcg cgccggtggt ggcc
//
LOCUS       DASVLN010000944         2348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_210807, whole genome shotgun sequence.
ACCESSION   DASVLN010000944 DASVLN010000000
VERSION     DASVLN010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210807"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VF453_22160"
     CDS             complement(<1..625)
                     /locus_tag="VF453_22160"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7690428.1"
                     /translation="MTQRPRGHAALETGRASIRDVAARAGVSVTTVSHALNGTRFVSP
                     DATARVQEAAHALGYVPSVVARGLKQNTTRTLGMLVPNNSNPYFAEIIRGVEHECYGA
                     GYSLVLANSNDDPQRQVDHLRVLAERRVDGIVLVASGEDADILASCKDLRLPLVLLDR
                     EIDGLDADLIEVDHAGGAALATAHLLGLGHPRVACIGGPAELRPSAQR"
     gene            complement(747..1715)
                     /locus_tag="VF453_22165"
     CDS             complement(747..1715)
                     /locus_tag="VF453_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7690429.1"
                     /translation="MSSTIRSLRQSLAGAVGTVAGLLVALALMIAFFSWRSEYFFTLD
                     TFVTIANDIPAVAVSAVGMTFVLIIAGIDLSVGSVMALAAAAAGIAMLQWGWSLLPAT
                     LLAMAVGFVVGVANGAITVAWRLPSFIVTLGMLEVARGGAYIATDSRTQYIGDRIDWL
                     GRPLAAGLSPQFFIAIAIVVLAQVVLTRTVFGRCLVGIGTNEEAMRLAGVDPRPIKIA
                     VFAIAGLLAGLGGVFLSSRLEAADPNAGTGAELVVIASVVIGGTSLMGGRGSVASTLF
                     GVLVISVLEAGLAQIGASEPTKRIITGVVIIVAVVLDTLRERRKKA"
     gene            complement(1712..>2348)
                     /locus_tag="VF453_22170"
     CDS             complement(1712..>2348)
                     /locus_tag="VF453_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7690430.1"
                     /translation="LRLIYGADRADAGTVALGDGRPLKARSPMRAVRAGIGLVTEDRK
                     SQGLLLPQPIRVNATLSNLEAVARGGWLRADAERAEVERLRELLRVRSDSIEQPVDQL
                     SGGNQQKVVFARWLHRDCDVLLLDEPTRGVDIGARADIYAQLDRLADAGKALLMVSSD
                     LRELMSVCDRIAVMSAGRLVGTFERGQWTQQALLEAAFSAYAQAESMEHAT"
BASE COUNT          337 a          844 c          864 g          303 t
ORIGIN      
        1 cgcgctgggc gctcggacgc agctcggccg gcccgccgat gcaggcgacg cgcgggtggc
       61 cgaggcccag caggtgcgcg gtcgccagcg ccgcgccgcc cgcatggtcg acctcgatca
      121 ggtcggcgtc caggccgtcg atctcgcgat cgagcagcac cagcggcagc cgcaaatcct
      181 tgcacgaggc gaggatgtcc gcgtcctcgc cggaggccac gagcacgatg ccgtcgacgc
      241 ggcgctcggc cagcacgcgc aggtggtcga cctggcgctg cgggtcgtcg ttggagttgg
      301 cgagcacgag gctgtagccg gcgccgtagc actcgtgctc caccccgcgg atgatctcgg
      361 cgaagtacgg attggagttg ttgggcacca gcatgcccag cgtgcgcgtc gtgttctgct
      421 tcaggccgcg cgcgaccacg ctgggcacgt agccgagcgc atgcgctgcc tcctgcacgc
      481 gggccgtcgc gtcggggctc acgaagcgcg tgccgttgag cgcgtgcgag accgtcgtca
      541 cggacacgcc cgcgcgtgcc gcgacgtcgc ggatgctggc gcggccggtc tcgagggcgg
      601 catggccgcg cggacgctgc gtcatcgccg gtctccgcgg gcgagccctg cgcgcgggtc
      661 gcgacccgag cattggggcg ggctcatcag gcgatctccg tcccgcggac tgcggtgcga
      721 gccccttcgg gcggccgggc gcgggctcat gccttcttcc gccgttcgcg cagcgtgtcg
      781 agcacgaccg ccacgatgat cacgacgccc gtgatgatgc gcttggtcgg ctcgctggcg
      841 ccgatctgcg ccaggccggc ctccagcacc gagatcacga gcacgccgaa cagggtgctc
      901 gccaccgagc cgcggccgcc catcaggctg gtgccgccga tgacgaccga ggcgatgacg
      961 accagctccg cgccggtgcc ggcgttcggg tcggcggcct ccaggcgcga ggacaggaag
     1021 acgccgccga gcccggcgag cagccccgcg atcgcgaaca ccgcgatctt gatcggcctc
     1081 gggtcgacgc cggccaggcg catcgcctcc tcgttcgtgc cgatgcccac caggcagcgg
     1141 ccgaacacgg tgcgcgtgag caccacctgc gcgagcacga cgatcgcgat cgcgatgaag
     1201 aactgcggcg acagccccgc cgcgagcggc cgcccgagcc agtcgatgcg gtcgccgatg
     1261 tactgcgtgc gcgagtcggt ggcgatgtag gcgccgccgc gggcgacctc cagcatgccc
     1321 agggtcacga tgaaggacgg cagccgccag gcgacggtga tcgcgccgtt ggccacgccg
     1381 accacgaacc cgaccgccat cgccagcagc gtcgccggca acagcgacca gccccactgc
     1441 agcatcgcga tgcccgcggc cgcggcggcc agcgccatca ccgagcccac cgacaggtcg
     1501 atgcccgcga tgatcagcac gaaggtcatg cccacggcgc tcacggcgac ggccgggatg
     1561 tcgttggcga tcgtgacgaa ggtgtcgagg gtgaagaagt actcgctgcg ccagctgaag
     1621 aaggcgatca tcagcgcgag cgcgacgagc agcccggcga cggtgccgac ggcgccggcc
     1681 aggctctggc gaagggatcg gattgtcgag ctcatgtggc gtgttccatg gattcggctt
     1741 gcgcgtaggc gctgaatgcg gcctcgagca gcgcctgctg cgtccactgg ccgcgctcga
     1801 aggtgcccac gagccggccg gcgctcatga cggcgatgcg gtcgcacacc gacatcagct
     1861 cgcgcaggtc ggacgacacc atcagcagcg ccttgccggc gtcggcgagg cggtcgagct
     1921 gcgcgtagat gtcggcgcgt gcgccgatgt cgacgccgcg cgtgggctcg tcgagcagca
     1981 gcacgtcgca gtcgcggtgc agccagcggg cgaagacgac cttctgctgg ttgccgcccg
     2041 acagctggtc caccggctgc tcgatcgagt cgctgcgcac gcgcagcagc tcgcgcaggc
     2101 gctccacctc ggcacgctcg gcatcggcgc gcagccagcc gccgcgggcg acggcctcca
     2161 ggttggacag cgtggcgttg acgcggatcg gctgcggcag cagcaggccc tgcgacttgc
     2221 ggtcctcggt gacgagcccg atgcccgcgc gcacggcccg catcggcgac ctcgccttga
     2281 gcgggcggcc gtcgccgagc gcgacggtgc ccgcgtcggc gcggtcggcg ccgtagatga
     2341 ggcgcagc
//
LOCUS       DASVLN010000945         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_265329, whole genome shotgun sequence.
ACCESSION   DASVLN010000945 DASVLN010000000
VERSION     DASVLN010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265329"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1646
                     /locus_tag="VF453_22175"
     CDS             276..1646
                     /locus_tag="VF453_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7690431.1"
                     /translation="MERKLATSRSVARTSGWQAWARRGVQALRERFALAPDDLLRDVV
                     YRRLWSSVLTSSFGAQIMLLALPLTAAVLLHASPTQMGALTTMEIAPFVLFSLPFGVW
                     LDRVRKLPVYIAGELTFALTALSVPLAWWAGLLSIRWLYVVAFTIGFVNTTAGSAAQI
                     VLTQVVRRDRLIEANAKNALASSGAEVAGPGMAGTLIKLLGAPVALLVDAALLVASAA
                     ILRGIRVVEEPASGRGAFWPELRAGVRFVAGNGLLVKLALCAGTWQFCYNAATVVNIL
                     FATRTLGMSEQAVGLSYVCIGVGTVSASLFGNRLSARLGPGPTMTLGFGACALGWLVL
                     ALAPVGPLGVAMLALDLTLFGVGAILIFINFISLRQAVTPAPMLGRMTSTMRWLILIP
                     AGPGALAGGWLGEHVGLRASLATAGGVAAVMTVLAWRSRTLSSIRVLPVLADASCETA
                     TEMLAP"
     gene            complement(1704..2357)
                     /locus_tag="VF453_22180"
     CDS             complement(1704..2357)
                     /locus_tag="VF453_22180"
                     /inference="COORDINATES: protein motif:HMM:NF016733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HEX7690432.1"
                     /translation="MFKSLLAAAALCSVAPAFAAANLLTDGSFESAVLPDGDYGMYGG
                     SSLYGWTAVGSGLIEVRRDIVGTAQDGDYFVELDSTANSAMSQTISTVAGQRYSLSFW
                     YSNRPASFSYNSVFPGGVAPASTNGLSFDVGSGAVNVPGLPANTSADNTWTLYSTSFV
                     ATGASTTLTFAATGTSDSWGSSIDDIAVTTAVPEPGSLALFGAGLAVLAGLARRRAR"
     gene            complement(2477..>2755)
                     /locus_tag="VF453_22185"
     CDS             complement(2477..>2755)
                     /locus_tag="VF453_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX7690433.1"
                     /translation="AAAAPGLPRALLLDELWDGWHEAAERLGCVAVVTHHPLMDAALV
                     RRLHAGGRRALVYTANAPADVARLRAAGVDGIITDEVQRFADDARRGT"
BASE COUNT          338 a          995 c         1018 g          404 t
ORIGIN      
        1 gcccgcgtcg cgccggccgc gcccgagccg atgccgccgt cgccgtcctg cggcaattcg
       61 cgcggcgtgg cgatgcaccc gtgcaagctc gccgccgccg gcggcccggg cttcacgccc
      121 gcgatctagt cctccccagc cgacccgggc acgacccgac gaccgctggg ccgccagcgg
      181 aacccgtcgt cgtcctcgcc gccttccgct gccaagaatc cgccgcccgt cgccgtccac
      241 gagacgcgcc gggggcgctc cagggagact cggacatgga aagaaaactg gccacgagcc
      301 ggtcggtagc ccgcacctcg ggatggcagg cctgggcccg ccgcggcgtg caggcactgc
      361 gcgaacgctt cgccctcgct cccgacgacc tgctgcgcga cgtcgtctac cggcggctgt
      421 ggagctccgt gctcaccagc tccttcgggg cgcagatcat gctgctcgcg ctgccgctga
      481 cggccgcggt gctgctgcac gcctcgccga cgcagatggg cgcgctcacg acgatggaga
      541 tcgcgccttt cgtgctgttc tcgctgccgt tcggcgtctg gctcgaccgt gtgcgcaagc
      601 tgccggtcta catcgccggc gagctcacgt tcgcgctgac ggcgctgagc gtgccgctcg
      661 cgtggtgggc gggcctgctg tcgatccgct ggctctacgt cgtggccttc acgatcggct
      721 tcgtcaacac gacggccggc agcgcggcgc agatcgtgct gacgcaggtg gtgcggcgcg
      781 accggctcat cgaggccaac gcgaagaacg cgctggcgag ttcgggcgcc gaggtcgcgg
      841 gccccggcat ggccggcacg ctgatcaagc tgctgggggc gccggtggcg ctgctggtgg
      901 acgcggcgct gctggtcgcg tccgcggcca tcctgcgcgg catccgcgtc gtcgaggagc
      961 ccgcctccgg acgcggcgcg ttctggcccg agctgcgcgc aggcgtgcgc ttcgtcgccg
     1021 gcaacgggct gctggtcaag ctcgccctct gcgcgggaac ctggcagttc tgctacaacg
     1081 ccgcgaccgt cgtgaacatc ctcttcgcca cgcgcacgct gggcatgtcg gagcaggcgg
     1141 tcggcctcag ctatgtctgc atcggcgtgg gcacggtcag cgcgagcctg ttcggcaacc
     1201 gcctgagcgc gcgcctcggg ccgggcccga cgatgacgct gggcttcggc gcctgcgcgc
     1261 tcggctggct ggtgctggcg ctcgcgcccg tcgggccgct gggggtggcg atgctcgcgc
     1321 tcgacctgac gctgttcggc gtcggggcga tcctgatctt catcaacttc atctcgctgc
     1381 gccaggcggt cacgccggcg cccatgctcg ggcgcatgac cagcacgatg cgctggctga
     1441 tcctcatccc tgccgggccg ggcgccctgg cgggcgggtg gctgggcgag cacgtcgggc
     1501 tgcgagcctc gctcgcgacc gccggcggcg tcgcggcggt gatgacggtc ctcgcctggc
     1561 gcagccgcac gctcagctcg atccgcgtgc tgcccgtgct ggcggacgcc agttgcgaga
     1621 ccgccaccga gatgctggcg ccctgacgcc ccggtgatga aaaggccgcc ccgcgggcgg
     1681 ccttccgctg cgcgtgcggg cgttcagcgc gcgcggcggc gggccaggcc cgcgagcacc
     1741 gcgaggccgg cgccgaacag ggccagcgag ccgggctcgg gcacggccgt cgtcacggcg
     1801 atgtcgtcga tcgagctgcc ccacgaatcg ctggtgccgg tggcggcgaa ggtgagcgtg
     1861 gtggacgccc cggtggcgac gaaggacgtc gagtacagcg tccaggtgtt gtcggcgctg
     1921 gtgttggcgg gcaggccggg cacgttcacg gcaccggagc cgacgtcgaa ggacagcccg
     1981 ttggtgctcg ccggcgcgac gccgcccggg aagacgctgt tgtacgagaa gctggccggt
     2041 cggttcgagt accagaagct gagcgagtag cgctggccgg cgaccgtcga gatcgtctgg
     2101 ctcatcgccg agttggcggt ggagtcgagc tcgacgaagt agtcgccatc ctgcgcggtg
     2161 cccacgatgt cgcggcgcac ctcgatcagg ccgctgccca ccgccgtcca gccgtacagc
     2221 gaactgccgc cgtacatgcc gtagtcgccg tcgggcagga cggcgctctc gaagctgccg
     2281 tcggtcagga ggttggcggc ggcgaaggcc ggcgcgacgc tgcacagcgc cgccgcggcg
     2341 aggaggctct tgaacatcgg tgggctccca attccaggtt tccgtgcagt attgctcgac
     2401 agcaacgccg cgggcagccc cgaaaagggt gagctcgacc gtgtttgtgc tagcggtgca
     2461 agtccgcgcg ctgcgctcag gtgccgcgcc gggcgtcgtc ggcgaagcgt tgcacctcgt
     2521 ccgtgatgat cccgtcgacg cccgcggccc gcaggcgcgc cacgtcggcc ggcgcgttcg
     2581 cggtgtagac cagcgcgcgc cgcccgcccg catgcagccg ccgcacgagc gctgcgtcca
     2641 tcagcggatg gtgcgtcacg acggcgacgc agcccagccg ctcggcggcc tcgtgccagc
     2701 cgtcccagag ctcgtcgagc agcagcgcac gcggcaaccc gggggcggcg gctgc
//
LOCUS       DASVLN010000946         5984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_320257, whole genome shotgun sequence.
ACCESSION   DASVLN010000946 DASVLN010000000
VERSION     DASVLN010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320257"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VF453_22190"
     CDS             complement(<1..470)
                     /locus_tag="VF453_22190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690434.1"
                     /translation="MTARIPGNRLSLDIPDDDLQAVQAALQVLHARLRPHLASLGSGE
                     RRRLARMGAKSVDFVAKNVGYARQHPELMPRFLDVEAFEHDFATVAKLRELQRSLAQL
                     HDLVGDSALLCGSDAYATALGCYRSIKAAAKLQRPGAATIAADLSTRFPGRPTRS"
     gene            complement(928..1545)
                     /locus_tag="VF453_22195"
     CDS             complement(928..1545)
                     /locus_tag="VF453_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690435.1"
                     /translation="MTTKTATGRALLKLSAARPTADRLQTAVQMSKVRYQAVSCRTMA
                     TNACCRPSAVFDDGRLSRLPVTYSQRPKQPQNAGMPDQARSAALTVELVKAFARELAS
                     IDRAWQRAFLRLAAQPGVTEVKASLVGSRGVEIVDVVAHKPFFHWANGIVGQLRDSLP
                     STSPFKVALVELNADFSYEVKYEFDNAQRWAISKLNGGSGLPTEY"
     gene            complement(1609..1989)
                     /locus_tag="VF453_22200"
     CDS             complement(1609..1989)
                     /locus_tag="VF453_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690436.1"
                     /translation="MSSNPYTPPRARTSDHLQDDQIDESVRRAGWLLFGTPFLVIAFI
                     IVASIFSLMYMRQGWIGYLVTELLLALLAFFSIQYALGRSASLLGSNWVLFGVVTILF
                     MPISTLIAWLTLVDKRAKLKRSQA"
     gene            complement(1919..2359)
                     /locus_tag="VF453_22205"
     CDS             complement(1919..2359)
                     /locus_tag="VF453_22205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690437.1"
                     /translation="MAVVGTATMCCSFIGLETLDNKLTHSTVASVADDEYGHLKDAVL
                     RSRPEVLRAMEQVAAQLGVVLKEAGSDDLLAIERRLIEDARAINNPDGSNRPGAVDDA
                     IHFFGRTKNARESQSREQGGNHVFKSIHAAESAYQRPPAGRSDR"
     gene            2892..3473
                     /locus_tag="VF453_22210"
     CDS             2892..3473
                     /locus_tag="VF453_22210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690438.1"
                     /translation="MKTRWLFVPFSAGHDDEVFRMARDWKSVEDWKGKRTVIARYANN
                     REGAGSLQNVVADDKLYVLGHGNVGCRAIANKFEDATEELGDTELCRRIEASGLPQGI
                     DLAVHLYSCGAGGRAEVVAGCGSGAVFAESFARTLRERFYYFGASVFGYTESVTSPER
                     NNDNGYEGHKLAVNHLGEILGRASEFRQPFKGY"
     gene            complement(3580..5094)
                     /locus_tag="VF453_22215"
     CDS             complement(3580..5094)
                     /locus_tag="VF453_22215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690439.1"
                     /translation="MPNQIDLAVTRLTNDLPQLFSVDENFVVHTTWLTGRNADAPWTP
                     WHFFPGRIKVIAATQMADGRPYLLGIHPDDNTLRANWKVSTDPDDAWSGWMVFDSPPL
                     ADVVAVPLVGGALQIFGIALDHSIVTCFDTSDTAEPAWSSWQPFAGALRKISAANLSD
                     GRPQLFGIATDGTVLTAWKTTLDTSAPWSQWTEMGLASCRDITVFPLSNGALQILACE
                     YGGPGKTCWKVGHDPDAAWSAWESFPDHTYVDAAASCRLPDGRAAIFGIDAGALTFNQ
                     KTTLSSAAAWTGWKPFLVNDVPPGPLTCAFAMESIQINSTRSLFKDTVFVSASLAIGP
                     YAPQTRTRAMGKLGNGLVQLDLAFDPIQMADKDTAVLTYAVVNNGHSDPGFVVQQLES
                     ATKKLAQKGADAVATAAGGALGAALGASIGTAAVPILGTAIGALAGWIVDTVGSALFA
                     NCDGTVAAGVHFITGAQMRAQGFMREQDYSDGTDSPNGCGDKSEYWVTWTVDSN"
     regulatory      5611..5705
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         1031 a         1931 c         2018 g         1004 t
ORIGIN      
        1 gaccgcgttg gccggcccgg gaagcgggtg gacaggtcgg cggcgatcgt cgccgcgccg
       61 ggacgctgca gcttcgcggc ggccttgatc gagcggtagc agccaagcgc cgtggcgtag
      121 gcgtcgctgc cgcacagcag cgcactgtcg ccgaccaggt cgtggagctg ggccagcgac
      181 cgctgcagct cgcgcagctt cgcgaccgtg gcgaagtcgt gctcgaacgc ctcgacgtcc
      241 aggaagcgcg gcatgagctc cggatgctgg cgcgcgtagc cgacgttctt cgcgacgaag
      301 tcgacggact tcgcgcccat gcgcgccagc cggcgccgtt ctccggagcc cagcgaggcg
      361 aggtgcggcc gcagccgggc gtgcagcacc tgcagcgccg cctggacggc ctgcaggtcg
      421 tcgtcgggaa tgtccaggga caggcggttg ccggggatgc gggcggtcaa gggcgtcttc
      481 ctcgcgaggg ctgggtggcg gggtgggggc gatggcgcgg cggtgattgt gcggatacat
      541 gccaaggtat ccagcctgtg acttaggggt ggacgacgtc ggcgacgggg ttccctgggg
      601 cggcacatgc tgcgcgacac gaatggcgcg gacgcgccac cccaccctgg gacgcggaag
      661 agggcggatg tccgcgaaaa gtccctgact ttcggtgcgg atcccggcgg tgcggcgcgg
      721 aaggccgggt tgcggcgccg gcgtggacgt ccgctgccgg gcatggggga gggcggctgt
      781 cgacccggag ccgacatcag taagtgcgat ctcagcgccg caaagccgac cttcccttgg
      841 tcgagcgtcc gacttcggct ttcgacccag agccgtcctg cgatctgacg atttggacgc
      901 cggaaagccg tcgttggttc atcgacctca gtattcggtc ggcagccccg atccgccgtt
      961 cagcttcgag attgcccatc gctgggcatt gtcgaactcg tacttcactt catacgagaa
     1021 atcggcattg agctcaacaa gcgctacctt gaacgggctg gtcgatggca gcgaatcgcg
     1081 aagctgcccg acgatcccgt tcgcccaatg gaagaaaggc ttatgcgcca cgacatcgac
     1141 tatctcgacg ccgcgcgagc cgacgagaga cgccttgacc tcggtgacgc cgggctgggc
     1201 cgcgagcctg agaaaggcgc gctgccaagc acgatcgata gacgccagct cgcgggcgaa
     1261 cgccttaaca agttcgacag tcagtgctgc gcttctggct tgatcgggca tgcctgcgtt
     1321 ttgcggttgc ttgggtcgct gcgagtatgt caccggcagt ctcgaaagcc gaccatcgtc
     1381 gaagaccgct gatggccggc agcaggcgtt ggtcgccatc gtacggcagc ttaccgcctg
     1441 gtagcgcacc ttcgacatct gaacggctgt ttggaggcgg tcagccgtcg gtcgcgcggc
     1501 gctgagcttc agcagggccc gacccgtggc ggtcttggtc gtcatcggat tcgtcgtcat
     1561 ggtgcggaca tgcgggaccg gcgtctcgca cctcgcgcgc cacccagttc aggcttggct
     1621 gcgtttgagc tttgctctct tgtctaccaa cgtcagccag gcaatgaggg tggagatcgg
     1681 catgaacaag attgtcacaa ccccaaacag cacccaattg gagccgagca acgaagctga
     1741 tcgcccaaga gcgtactgga tcgagaagaa cgctagcagg gcgagaagca gctctgtgac
     1801 cagatacccg atccagcctt ggcgcatgta catcaacgag aagatgcttg cgacgatgat
     1861 gaacgcgatc accaggaacg gcgttccgaa aaggagccaa cctgcacgcc gtaccgattc
     1921 atcgatctga tcgtcctgca ggtggtcgct ggtacgcgct ctcggcggcg tgtatggatt
     1981 tgaagacatg gttccctcct tgctcgcgag actggctctc gcgagcattc ttcgttctgc
     2041 cgaaaaaatg aattgcgtcg tcgacagctc ctggccggtt cgagccatca gggttgttga
     2101 tcgcacgcgc atcttcgatc aggcgccttt caatggcaag aagatcgtcg ctgccagcct
     2161 ctttgaggac gacgccaagc tgggcggcga cctgctccat agcacgcaag acctcggggc
     2221 ggctcctcaa caccgcgtct ttcaggtggc catattcgtc gtctgcaacg gaggcgactg
     2281 tcgaatgggt cagcttgttg tcaagggtct ccaagccgat gaagctacag cacattgtcg
     2341 ccgtgccgac cacggccacc tcagcctctg taagcggacg gttccaaaga cgcgccagcg
     2401 aggtttggat cgaccggaag aagacgtcgt ggatccgaag tcggcgttca agctgttcga
     2461 ggctccattc ttccgaagac tagaagggcc gaggaatcgg tggcctgaaa gcagacgccc
     2521 gcgaccgacg gctgcgtgcc ggctgcgcgc cgaccgtgtt cgatatccta cggcagctca
     2581 cggcctggaa gcgcaccttc gtcgccggaa cgtccgcctg gcggccatca gctgccgcca
     2641 gcctcttggt cgccttcagg cggggccgat ccgttgcgga catcatccgc acccaactca
     2701 ccgcccgaga gaagtcgttc gatacgcgaa tgagattctc ctctcgcgtc atggccatcg
     2761 tgtcgggtcc cggcctcgga ttccgttcta gctgatgacg cgcctcgtca cggggcgacg
     2821 ctcctgccga gccggcgggg tgcgcgctcg ccgtggcgcg ttcgcgcatc gaccctcacc
     2881 aggagactcc gatgaagacc cgctggcttt tcgtaccgtt ctctgccggg cacgacgacg
     2941 aagtgttccg gatggcgcgc gactggaagt ccgtcgagga ctggaagggc aagcgcacgg
     3001 tgatcgcgcg ttacgccaac aatcgcgagg gcgccggaag tctccagaac gtcgtcgcgg
     3061 acgacaagct ctacgtactc ggccacggca acgtcggctg ccgagcgatc gccaacaagt
     3121 tcgaggacgc caccgaggaa ctgggcgaca ccgaactgtg ccgccgcatc gaggccagcg
     3181 ggctgccgca gggtatcgac ctcgccgtcc acctctactc gtgcggcgcg ggaggccgcg
     3241 ccgaggtggt cgccggctgc ggctcgggcg ccgtctttgc cgagtccttc gcgcggacgc
     3301 tgcgcgaacg cttctactac ttcggtgcca gcgtcttcgg ctacaccgag agcgtcacct
     3361 cgcccgagcg caacaacgac aatggctacg aaggccacaa gctcgcggtc aaccacctcg
     3421 gcgagatcct cgggcgggcg agcgagttca ggcagccgtt caagggctat tgaccctcgg
     3481 cgattgccgg gcaagcgttc gatcgcggca tcagggcgac gattgccttg cggatcgtcc
     3541 gtggaccagg gattcgaagc gcatcgcctg cagggcgtct cagttcgagt cgaccgtcca
     3601 ggtcacccag tactccgact tgtctccgca gccattgggt gagtcggtgc catcgctgta
     3661 gtcctgctcg cgcatgaagc cctgcgcccg catctgcgcg cccgtgatga agtggacgcc
     3721 ggccgccacg gtaccgtcgc aattggcgaa cagggcgctc ccgacggtat cgacgatcca
     3781 gcccgccagg gcgccgatcg ccgtgccgag aatcggcacc gccgccgtgc cgatgctggc
     3841 acccaacgcg gcccccaacg caccgcctgc agccgttgcc acggcgtccg cgcctttctg
     3901 tgccaatttc ttggtcgcgc tttccagttg ctgcacgacg aagcccggat ccgaatgccc
     3961 gttgttcacg acggcatagg tcagcaccgc ggtgtccttg tccgccatct ggatcggatc
     4021 gaaggcgagg tcgagctgga cgaggccatt gcccagcttg cccatggcgc gcgtcctcgt
     4081 ctgcggcgca taggggccga tcgccaggct cgccgagacg aagaccgtgt ccttgaacag
     4141 cgaacgcgtg ctgttgatct ggatggactc catcgcgaag gcgcaggtca ggggccccgg
     4201 cggcacgtcg ttcacgagga agggcttcca cccggtccag gcggctgccg agctgagcgt
     4261 cgtcttttga ttgaaggtga gcgcgccggc gtcgattccg aaaatggccg cccgtccgtc
     4321 aggcaggcgg cagctcgccg cggcatccac atacgtgtgg tcgggaaacg attcccaggc
     4381 cgaccatgcc gcgtccgggt cgtgcccgac cttccagcac gtcttgccgg gcccgccgta
     4441 ttcacacgcg aggatctgga gcgcaccgtt ggagagcgga aacacggtga tgtcgcggca
     4501 gctcgccaat cccatctccg tccattgcga ccacggggcg ctggtgtcca gcgtggtctt
     4561 ccaggccgtc agcacggtgc cgtccgtggc gatgccgaac agctgggggc gaccgtcgga
     4621 gaggttggct gcggagatct tcctgagcgc accggcaaag ggctgccagg aactccacgc
     4681 aggctccgcc gtgtcggagg tatcgaagca ggtcacgatc gagtggtcga gcgcgatgcc
     4741 gaagatctgc agcgctccgc cgaccagggg caccgccacg acgtccgcca gcggaggcga
     4801 gtcgaagacc atccagccgg accacgcatc gtccggatcc gtcgagactt tccagttggc
     4861 tctcaacgtg ttgtcgtcgg gatggatgcc gagcagatat ggcctgccat ccgccatctg
     4921 cgtggccgcg atcaccttga tccggccggg aaagaaatgc cagggcgtcc atggcgcatc
     4981 ggcgttccgg ccggtcaacc aggtcgtgtg gacgacgaag ttctcatcga cggaaaacag
     5041 ctgcggcagg tcgttggtca ggcgggtgac tgcaaggtcg atctggtttg gcatggacaa
     5101 tctcgcttga ggtgaagaat cgcgagcccg gctgagcaat catcgaagca cgagattcgg
     5161 tacgggcggt gccgggcaat gaggcccggc atacctgtgc ctccttgtac gagatgggtg
     5221 caggtttccg gacacggcat gcccgcgggg gcgcgccgcg acgcgcttgc cgggggcgat
     5281 cggctgccag acctcagcca cgtcgccctg tggcagccgc gccgatcgga tggtcggcag
     5341 gagtgcgggg tcgatcggcg gggccccgtc gtgcaccgcc agacgttgcg ctgcccggaa
     5401 gggcacgtgg gcacccatgc atcgaggatc ccggacacca cgtcgcggga gatgccgatc
     5461 ttgacgcatg cctatacggg taggtgtatc gaagacggtg ccgaccatcg tcgcgcgacc
     5521 gtcgaataga tcacgccgcc gcgtccttgt cgcgttgcag gccgcggctc gcgcgccgat
     5581 aagcctcgtg cgagggagcg ccgacggcgc ggcccgcaaa ggcaaacccg accgaaaggc
     5641 ggggacgcaa agcttccggt ctaccgcgcg tgcgacgcac gcgacacgac agcggggttg
     5701 ccgcgaggcg accgggcatg cgccggctcg tccgatccga tgcgctgcgc gttcgcgcgg
     5761 cggcggcagc ccctggatgc acgtgatccc gccgcccggt cccaggacga gacagggaca
     5821 cgaccgacat gggctccaac gaccaattcc attccccggc cgccgaggaa ctgctgcgcc
     5881 ggctcggccg ccggcagggc ggacggccgg gcatccgctg gacgcaggac gacgtcgcgc
     5941 ggctggtcga acgcgtgctg ccgacgccga gcggccgcat gaac
//
LOCUS       DASVLN010000947         6234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_265366, whole genome shotgun sequence.
ACCESSION   DASVLN010000947 DASVLN010000000
VERSION     DASVLN010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265366"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VF453_22220"
     CDS             complement(<1..393)
                     /locus_tag="VF453_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEX7690440.1"
                     /translation="MAVDMFMKIDSLKGESNDKTHKGEIDVLAWSWGGSNSGSAHVGG
                     GAGSGKANVQDLSFTKYIDKSTPDLLLSCFNGKHWDSATLVVRKAGETPLEYLKITMT
                     EVLVTSVSQGGSGGEDRLTENVTLNFASV"
     gene            complement(461..1951)
                     /gene="tssC"
                     /locus_tag="VF453_22225"
     CDS             complement(461..1951)
                     /gene="tssC"
                     /locus_tag="VF453_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEX7690441.1"
                     /translation="MSTQQESSAASGATAEFDDFAALLQKEFKPKTDQAKNAVESAVH
                     TLAQQALADTALVSNDVYNTIEALIAALDKKLSEQVNLIIHHADFTKLEGAWRGLHHL
                     VNNTETDEMLKIRVMNISKGELHKTLKRYKGTAWDQSPIFKKVYEEEFGQLGGTPYGC
                     LVGDYHFDHSPVDVELLGEMAKIAAAAHAPFIAGASPTTLQMDSWRELSNPRDLTKIF
                     DNSEYAAWRSLRESEDSRFIGLAMPRFLARLPYGAKTNPVDAFDFEEEVEGSEHNSYV
                     WANSAYAMATNITRAFKLYGWCTTIRGVESGGAVPNLPVHSFPTDDGGVDMKCPTEIA
                     ISDRREAELAKNGFMPLVHRKNSDFAAFIGAQSLQKPAEYDDPDASANANLAARLPYL
                     FASCRFAHYLKCMVRDKVGSFATAQSMQRYLSDWIGNYVVSNAESAGQLIQAQKPLSA
                     AEVVVEEVEGNPGYYTSKFFLKPHYQLEGMSISLRLVSKLPSSKAA"
     gene            complement(2001..2510)
                     /gene="tssB"
                     /locus_tag="VF453_22230"
     CDS             complement(2001..2510)
                     /gene="tssB"
                     /locus_tag="VF453_22230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEX7690442.1"
                     /translation="MSGSSQKFIARNRAPRVQIEYDVELYGSNKKMQIPFVMGVMADL
                     SGKPAEPLAPVADRKFLEIDVDNFDERLKSMKPRVAFAVPNTLTGEGNLSVDITFESM
                     ADFSPEAVAKKVGALNNLLEARTQLKDLLTYMDGKVGAEELLGKVLKNPELLKSLATA
                     PKPTDAPAE"
     gene            complement(2545..3630)
                     /gene="tssA"
                     /locus_tag="VF453_22235"
     CDS             complement(2545..3630)
                     /gene="tssA"
                     /locus_tag="VF453_22235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEX7690443.1"
                     /translation="MLDLDALLRPIDDAEPAGPDLEYSDVAELDRFAAGTPGTIDPKT
                     QELVGAEEPNWRKVAEMSKDLLGKTKDLRVAVWLARAELANRGLPGLADGLKLIARLC
                     EDFWETLYPVLDRDYDNDPMERMNALANLSPDPAQSYGSPQAEGMLRLIRGTAIVESR
                     EVGRYTVRDLEYVLGRMQPPEGQTAPQEGLLVAAWQHGDPEANQEKRDGIQVGLAAIQ
                     AIDRLFNDHSGQRPGLDLLQQTFKRVGDFYAAHEVDDGSGAGDAVAAEGGDDGDAGGD
                     GAGAGKSPRSGGLASRADAVRILQQVAEFLRKAEPSSPAPMFVDRAIKLLQMDFNAIV
                     RELMPDSKDRIEMLGGISLDDPESDDD"
     gene            complement(3752..4852)
                     /locus_tag="VF453_22240"
     CDS             complement(3752..4852)
                     /locus_tag="VF453_22240"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX7690444.1"
                     /translation="MDDDLETVRQFRKNILGVDGLFSTDDASRFQGKSGALMFKPLSG
                     FGFIAAGTGKFSGDMLIATRGTDTTADWLTDFNVGVQKGPGGEWVHAGFNTTWKSFAP
                     QIKEFMRGRNPSRIHLVGHSLGGALAFLNADYIAAERIAGVCLYTFGAPRAGTNAFAN
                     KLTDYIGADNIYRVSNPVDPVPMIPIFPFSHIPFNVEGLQIARTSHIPINPLGHMMAK
                     AYVPGVAGKEWNNLRIAAPSDRDVQDWLAEAAEGKGGMMGSAKLLDMIGRAIRYMLKM
                     AGTVVNVGVGGIATGAVTLLDWVAYMLTKAAQISLELGTGLVTLVNMIFRFLGRKAIQ
                     GAQITTAFLRWVLDLLFSSLSAAARRALSIIG"
     gene            complement(4940..5401)
                     /locus_tag="VF453_22245"
     CDS             complement(4940..5401)
                     /locus_tag="VF453_22245"
                     /inference="COORDINATES: protein motif:HMM:NF042847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6795 domain-containing protein"
                     /protein_id="HEX7690445.1"
                     /translation="MKRRGVWMSIGAVVVLLGVMQGTAMGKKVIWSAMKGRVLLNGQP
                     AAGAILKRDFNWAWTREHGVDETTTNSAGEFSLPSIERTSILGSILPHEPVIRQVMTI
                     TYKGVEYNAWANYKSDYDLNSENGGRPIVVTCRLEAERKEHGEVMGLCEFN"
     gene            complement(5465..5947)
                     /locus_tag="VF453_22250"
     CDS             complement(5465..5947)
                     /locus_tag="VF453_22250"
                     /inference="COORDINATES: protein motif:HMM:NF042847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6795 domain-containing protein"
                     /protein_id="HEX7690446.1"
                     /translation="MSALLASRGLWIVLGIVAALFVVVQVTGFGKVVIWSAMKGRVLM
                     NGQPVVGAVLTREYFWHWPNQKGSDRAKTNAAGEFSFPAIERRMILGMVLPHEPVIEQ
                     EMTIEHNGSSYQAWAHFKRDYEPNDENNGHPIDVTCRLGEERARHGLVMGLCEFNEVR
                     "
     gene            complement(5944..>6234)
                     /locus_tag="VF453_22255"
     CDS             complement(5944..>6234)
                     /locus_tag="VF453_22255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690447.1"
                     /translation="DEAAWFAAEEAALAGLDPALPMDDFDRALQALLEPGAGATDAAA
                     DPGAAVHFFSAGSDEAPPLRLAFPALPPASAFVDLLVTPPPPASSLMETLAS"
BASE COUNT         1023 a         2020 c         2024 g         1167 t
ORIGIN      
        1 gaccgaggcg aagttcagcg tgacgttctc cgtcaggcgg tcctcgccgc ccgagccgcc
       61 ttgcgacacc gaggtcacga ggacttcggt catcgtgatc ttcaggtact cgagcggggt
      121 ctcgccggcc ttgcgcacga cgagcgtcgc cgaatcccag tgcttgccgt tgaagcacga
      181 cagcagcagg tccggcgtcg acttgtcgat gtacttcgtg aaggacaggt cctgcacgtt
      241 ggccttgccg gagccggcac cgccacccac gtgcgccgag ccggagttcg aaccgcccca
      301 gctccaggcc aggacgtcga tctcgccctt gtgggtcttg tcgttcgact cgcccttgag
      361 ggaatcgatc ttcatgaaca tgtcgactgc catttttcta ctccttagaa cattgaattc
      421 ccgtcacgca ggacaggaat gcgccccaat tgactgggac tcaagcggcc ttgctggaag
      481 gcagcttgga aacgagccgg agggagatgc tcatcccctc cagctgatag tgcggcttca
      541 ggaagaactt cgaagtgtag tagccggggt tgccctcgac ttcctcgacg acgacttccg
      601 cggccgacag cggcttctgg gcctggatca gctggcccgc gctctccgcg ttggagacca
      661 cgtagttgcc gatccagtcg ctcaggtagc gctgcatcga ttgggcggtg gcgaacgagc
      721 ccaccttgtc gcgcaccatg cacttcaggt agtgcgcgaa gcggcaggag gcgaacaggt
      781 acggcaggcg cgcggccagg ttggcgttgg cgctggcgtc cggatcgtcg tactcggccg
      841 gcttctgcag cgactgggcg ccgatgaagg cggcgaagtc gctgttcttg cgatggacga
      901 gcggcatgaa gccgttcttg gccagttccg cctcgcggcg gtcggagatc gcgatctcgg
      961 tcgggcactt catgtccacg ccgccgtcat cggtcgggaa cgaatgcacc ggcaggttgg
     1021 gcaccgcgcc accggactcg acgccgcgga tcgtggtgca ccagccgtac agcttgaacg
     1081 cgcgggtgat gttggtggcc atcgcgtagg ccgagttggc ccacacgtag ctgttgtgct
     1141 ccgaaccctc gacttcttcc tcgaagtcga acgcgtcgac cgggttggtc ttcgcgccgt
     1201 acggcaggcg cgccaggaag cgcggcatgg ccaggccgat gaagcgcgaa tcctcggact
     1261 cgcgcagcga acgccacgcg gcgtactcgg agttgtcgaa gatcttggtc aggtcgcgcg
     1321 ggttggacag ctcgcgccac gaatccatct gcagcgtcgt cggcgaggca ccggcgatga
     1381 agggggcgtg cgcggcggcc gcgatcttgg ccatctcgcc cagcagctcg acgtcgaccg
     1441 ggctgtggtc gaagtggtag tcgccgacca ggcagccgta gggcgtgccg cccagctggc
     1501 cgaattcctc ttcgtagacc ttcttgaaga tcgggctctg gtcccaggcg gtgcccttgt
     1561 agcgcttgag cgtcttgtgc agctcgccct tcgagatgtt catcacgcgg atcttgagca
     1621 tctcgtccgt ctcggtgttg ttcacgagat ggtggagacc ccgccacgcg ccttcgagct
     1681 tcgtgaagtc ggcgtggtgg atgatgaggt tgacctgctc ggacagcttc ttgtcgaggg
     1741 ccgcgatgag cgcctcgatc gtgttgtaga cgtcgttgga caccagcgcg gtgtcggcca
     1801 gcgcctgctg cgccagcgtg tggacggcgg actcgacggc gttcttcgcc tggtcggtct
     1861 tcggcttgaa ctccttctgc agcagcgccg cgaagtcgtc gaactccgcg gtcgcgccgg
     1921 aggcggcgga cgattcttgt tgcgtggaca tctcaggaac tcctcggatc tgtcgtggct
     1981 gggcgtgcgg acccgacggc ttattcggcc ggcgcgtcgg tcggcttcgg cgcggtggcc
     2041 agcgacttca gcagttccgg gttcttcagg accttgccga gcagttcctc ggcgccgacc
     2101 ttgccgtcca tgtaggtcag caggtccttc agctgggtgc gcgcttccag caggttgttc
     2161 agcgcgccga ccttcttggc gaccgcttcc ggcgagaagt ccgccatgct ttcgaacgtg
     2221 atgtccacgc tcaggttgcc ttcgcccgtg agcgtgttgg gcaccgcgaa cgcgacgcgc
     2281 ggcttcatcg acttcaggcg ctcgtcgaag ttgtcgacgt cgatctcgag gaacttgcgg
     2341 tccgcgaccg gcgcgagcgg ctccgcgggc ttgcccgaca gatcggccat cacgcccatc
     2401 acgaacggga tctgcatctt cttgttcgac ccgtacagct cgacgtcgta ttcgatctga
     2461 acgcgcgggg cgcggttgcg ggcgatgaac ttctggctgc ttcctgacat gacctctctc
     2521 cttgactaac gtgtgaaccc ggaatcaatc gtcgtccgat tccgggtcgt ccagcgaaat
     2581 gcctcccagc atctcgatgc gatccttgga atcgggcatc aattccctga cgatcgcatt
     2641 gaaatccatc tgaagcagct tgatcgcccg gtcgacgaac atcggtgccg ggctgctcgg
     2701 ttcggccttg cggaggaact cggccacctg ctgcaggatt ctcactgcat ccgcgcgcga
     2761 tgccaagcca cccgaacggg gggatttccc cgcccccgcg ccgtcgccgc cggcgtcgcc
     2821 gtcgtctcca ccctccgcgg ccaccgcgtc gccggccccc gagccgtcgt cgacctcgtg
     2881 cgcggcgtag aagtcaccca cgcgcttgaa ggtctgctgc aggaggtcga ggcccgggcg
     2941 ctggccgctg tggtcgttga acaggcggtc gatcgcctgg atcgccgcca gcccgacctg
     3001 gatgccgtcg cgcttttcct ggttggcctc ggggtcgccg tgctgccagg cggcgacgag
     3061 caacccctcc tgcggcgcgg tctgcccctc gggcggctgc atgcggccaa ggacatactc
     3121 gaggtcgcgc accgtgtagc ggcccacctc gcgcgattcc acgatggccg tgccgcggat
     3181 caggcgcagc atgccctcgg cctgcggcga gccgtaggac tgggcgggat cgggcgacag
     3241 gttggcgagc gcgttcatgc gctccatggg atcgttgtcg tagtcccgat cgagcaccgg
     3301 gtacagcgtc tcccagaagt cctcgcacag gcgcgcgatc agcttcaggc cgtccgccag
     3361 gcccggcagg ccgcggttgg cgagctcggc gcgcgcgagc cagacggcca cgcgcaggtc
     3421 cttggtcttg ccgaggaggt ccttggacat ctcggcgacc ttgcgccagt tcggctcctc
     3481 ggcgccgacc agctcctgtg tcttcgggtc gatcgtgccg ggcgtgccgg cggcgaatcg
     3541 gtcgagctcc gcgacgtcgg agtattcgag gtcgggcccg gcgggttcgg catcgtcgat
     3601 gggacgcagc agcgcgtcaa ggtccagcac gtcagggatt ctccgttgga atgcgacgcg
     3661 ccgactgcag agcagcgcga gggcggagcg tacggcaagt cgatgacatc ttcaagatga
     3721 tccgaccgac ttgcgcggtc gcggggacgg ttcagccgat gatcgacagg gcgcggcgcg
     3781 ccgcggcgct cagcgacgag aacaggaggt cgagcaccca gcgcaggaac gccgtcgtga
     3841 tctgcgcgcc ctggatggcc ttgcggccga ggaagcggaa gatcatgttg acgagggtga
     3901 cgaggccggt gccgagttcg agcgagatct gggcggcctt ggtcagcatg taggcgaccc
     3961 agtccagcag ggtgacggcg ccggtcgcga tgccgcccac accgacgttg accaccgtgc
     4021 ccgccatctt cagcatgtac ctgatcgccc gtccgatcat gtccagcagc ttggcagagc
     4081 ccatcatgcc gcccttgcct tcggctgcct ccgcgagcca gtcctggacg tcgcggtcgc
     4141 tcggcgccgc gatccgcagg ttgttccact ccttgccagc tacaccgggg acgtacgctt
     4201 tggccatcat gtggcccaaa gggttgatgg ggatgtgaga cgtccgcgcg atctgcaggc
     4261 cttcgacgtt gaacgggatg tggctgaagg ggaagatcgg gatcatcgga accgggtcga
     4321 ccgggttcga aacgcggtag atgttgtcgg cgccgatgta gtcggtcagc ttgttcgcga
     4381 aggcgttcgt gccggcgcga ggagcgccga aggtatacaa gcagactccg gcaatacgct
     4441 cggcagcgat gtagtcggca ttgaggaagg ccaacgcacc gcccaggctg tgcccgacca
     4501 agtgaatgcg ggacgggttg cgcccacgca tgaattcctt gatctgcggg gcgaaagact
     4561 tccaggtcgt gttgaagcct gcgtgcaccc actcgccgcc cggccccttc tggacgccga
     4621 cattgaaatc agtcagccaa tccgccgtcg tgtcagtacc acgagtggca atcaacatgt
     4681 caccggagaa tttgcccgta ccagcggcaa tgaaaccgaa accgctgagc ggcttgaaca
     4741 tcagcgcgcc agacttgccc tgaaaccgag aggcgtcgtc cgtgctgaat aagccgtcaa
     4801 cgccgaggat gttcttacga aactgacgaa cggtctccag atcgtcatcc atgagacgat
     4861 agacgccggc agcgattgcg gccgcttgag ccgggctaag tgtagtcatt gttccttccc
     4921 tttctcttca acaacgggtt caattgaatt cgcagagtcc catcacctcg ccgtgctctt
     4981 ttcgctccgc ctcgaggcgg cacgtcacga cgataggacg gccgccgttc tcggagttga
     5041 ggtcatagtc gctcttgtag ttcgcccagg cgttgtattc gacacccttg taggtgatgg
     5101 tcattacctg tcggatcacc ggctcatgcg gcaggatgct gccaaggatc gaggtacgct
     5161 cgatcgacgg gagcgagaat tcgcccgctg aattggtggt cgtctcgtcg acgccatgct
     5221 ccctcgtcca agcccaattg aagtcgcgct tgaggatcgc acccgccgct ggctggccat
     5281 tcagcagtac acggcccttc atcgccgacc aaatcacctt ttttcccata gcagtccctt
     5341 gcatcactcc gagcagcacc accacggcgc cgatggacat ccagacaccg cgtcgcttca
     5401 tcgcacgact ccctgtcgcc cgcgcgacgc attaccgatg ttcgagcctc tcacgatacg
     5461 ggcctcaccg gacttcgttg aattcgcaca atcccatcac cagaccgtgg cgagcccgct
     5521 cctcaccgag gcggcaggtc acgtcgatgg gatgaccgtt gttttcgtcg ttgggctcat
     5581 agtcgcgctt gaagtgagcc caggcctggt acgagctgcc gttgtgctcg atcgtcattt
     5641 cctgctcaat caccggctcg tgcggcaaga ccatgcccag gatcatccgt cgctcgatcg
     5701 ctggaaatga gaactcacct gccgcattgg tcttcgctcg atcggagccc ttctggttcg
     5761 gccaatgcca gaaatattcc cgcgtcagca ccgcccccac cacaggctgc ccgttcatca
     5821 gcacccgccc cttcatcgcc gaccagatca cgaccttgcc gaagccggtc acttgcacga
     5881 cgacgaacag cgccgcgacg atgccgagga cgatccagag tccgcgcgag gcgagcaagg
     5941 cgctcatgaa gcgagcgtct ccatcagcga cgacgccggc ggcggcggcg tcacgaggag
     6001 atcgacgaag gccgacgccg gtggcagcgc aggaaaggcc aggcgcagtg gcggcgcctc
     6061 gtccgagccg gcgctgaaga aatgcacggc ggcgcccggg tcggcggcgg cgtcggtcgc
     6121 gccggcgcct ggctcgagga gggcctgcag cgcgcggtcg aagtcgtcca tcggcagcgc
     6181 gggatcgagt cccgcgagcg cggcctcctc ggcggcgaac caggccgcct cgtc
//
LOCUS       DASVLN010000948        14284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_47909, whole genome shotgun sequence.
ACCESSION   DASVLN010000948 DASVLN010000000
VERSION     DASVLN010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14284
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47909"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VF453_22260"
     CDS             <1..384
                     /locus_tag="VF453_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7690448.1"
                     /translation="RPLQAVVREVAAAADPATRTFQVKAGLPAGAAELGQTATVHVEL
                     APADGKLRLPLQAVTGEGGRSQVWVVDKATMTVREQPVKVLRPEGDSLVLDGGLKAGD
                     IVVTAGAHVLTPGQQVAFYVEPAKR"
     gene            392..3604
                     /locus_tag="VF453_22265"
     CDS             392..3604
                     /locus_tag="VF453_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7690449.1"
                     /translation="MSHDTPGGMTSRFNISRWALQHPELTRYLMIVLLVLGLAAYFQL
                     GQDEDPPFTFRAMVVQVYWPGASAKEMAEQVTDKIERTLQEVPYSDKIRSYTKPGESL
                     TILQLKDSSPPHEVANTWYQARKKIGDMRGTLPQGVAGPFFNDEFGDVYGSIFALSAD
                     GFSDEELRVRAEDIRQQLLRVPDVAKVQLFGVQSQKVFVELSQKRAAEMGIDLNQVVS
                     QLNAQNVVAGTGVVDAGAQNVQVRLSGAFQSVDAIRAFPIRATNPATGQSTTVRLGDI
                     ADVKRGYSDPPDVEVRANGKQVVALGVSMAKGGDIISLGKALQSQVGEIRRQLPVGME
                     ITQVQDQPRAVARSVGEFVRVLVEAIVIVLVVSFVSLGLHFNPIRIDIWPGLVVAITI
                     PLVLAITFVTMFYWGVGLHKISLGSLIIALGLLVDDAIIAVEMMVRKLEEGYSKERAA
                     TFAYEVTSMPMLTGTLITAAGFLPIGLAKSMTGEYTFAIFAVTSAALVISWMVSVYFV
                     PYLGAKLLHTRAKPHAGDGVPEEPHETFDSPFYGRFRALVAWCLRHRWITIGATIGTF
                     VLGIVGMGLVQQQFFPDSSRPEVMVDLWLPEGATIRQSEAVAKRFEARMMKEPGVTTV
                     TTWVGSGVPRFYLPLDQIFPQSNVSEVIIEPTDLKTREALRKKLPALLAAEFPEVRTR
                     VKLLPNGPPVPYPVQFRVMGQDVEQVRHWGDLAKDVLRANAHMRGVNDNWDENVLATR
                     LEIDQDKARALGVSTQSIAQATQTQFSGSTIGQYREGDQLIDIVLRQPAAERGKVTDL
                     SSAYVTAANGSAVPLAQVAHLALGWEPGVLWREGRDYAVTVQGDVVEGMQGPTVTAEL
                     WPALKALQAKMPPGYQIQVAGAVEESSKGQGAIMAGMPVMLFIIFTLLMLQLHSFSRA
                     VLVFLTGPMGIAGAAGALLALNRPFGFVAMLGVIALMGMIMRNSVILIDQIEQERARG
                     VPAWNAVIESVVRRFRPIVLTAAAAVLAMIPLSESEFWGPMAVAIMGGLVIATALTLL
                     ALPAMYAAWFRVREDVPAPSADAAAQHREGARTLASPAGA"
     gene            3858..3944
                     /locus_tag="VF453_22270"
     tRNA            3858..3944
                     /locus_tag="VF453_22270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3892..3894,aa:Leu,seq:tag)
     gene            3970..5277
                     /gene="tig"
                     /locus_tag="VF453_22275"
     CDS             3970..5277
                     /gene="tig"
                     /locus_tag="VF453_22275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX7690450.1"
                     /translation="MAVNVETLDKLERRISLTLAADEVNKEVEARLKKLSRTVKADGF
                     RPGKVPMSVVAQRYGYSVQYEVMNDKLGAAFAAAANEAKLRVAGQPRIAEKAQSAEGA
                     MEFDATFEVYPEVVIGDLSQVEIERVSTEVDEAAIDKTVDILRKQRRTFAQRPAAEGA
                     EAGDRVTIDFEGKIDGTPFDGGKAENFQFMVGEGQMLEQFDQAVRGMKVGESKTFPLQ
                     FPADYHGKEVAGREADFLVTMKKIEAQHLPEVDDAFAKSLGIAEGTVEGLRADVRKNL
                     EREVKFRVLARNKAAAMDALANAATLDLPKALVEGETERLVQGAREDLKQRGVKDADK
                     APIPAEIFTPQAERRVRLGLVVAELVRAQNLQAKPEQLQAHIEEMSQSYEKPVEVQRW
                     YLSDRSRMAEVEAIVVENNVTDYVLGQAKVTDKVLPFDQLMTA"
     gene            5513..6121
                     /gene="clpP"
                     /locus_tag="VF453_22280"
     CDS             5513..6121
                     /gene="clpP"
                     /locus_tag="VF453_22280"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX7690451.1"
                     /translation="MSALETQALGMVPMVIEQSGRGERAYDIYSRLLRERIVFLVGPV
                     NDQSANLVVAQLLFLESENPDKDIHLYINSPGGSVSAGMSIFDTMNFIKPHVSTLCIG
                     LAASMGAFLLAAGEKGKRLSLPNSKIMIHQPLGGAQGQATDIEIHAREILKTREQLNR
                     ILAERTGQPLEKIQADTERDYFMSADDAKSYGLVDQVVANRD"
     gene            6219..7487
                     /gene="clpX"
                     /locus_tag="VF453_22285"
     CDS             6219..7487
                     /gene="clpX"
                     /locus_tag="VF453_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX7690452.1"
                     /translation="MADKKGSSAEKVLYCSFCGKSQHEVKKLIAGPSVFICDECIELC
                     NDIIRDEVPAEGPAAKSAKSDLPMPSEIKASLDNYVIGQDVAKRTLSVAVYNHYKRLK
                     HMSTSKDDVELSKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGED
                     VENIIQKLLQNCNYDVERAQRGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKLV
                     EGTMASVPPQGGRKHPNQDFLQIDTTNILFICGGAFDGLEKVIRNRTERSGIGFGATV
                     HSKDERRVSELFREVEPEDLIKFGLIPELVGRLPVVATLGELTEEALVQILTEPKNAL
                     VKQYQKLFMMDGVELEIRPSALAAIARKALARKTGARGLRSIMEQALIDTMFDLPLLD
                     GVAQVVIDEHIIEENAKPLLVYREQAKASA"
     gene            7874..10303
                     /gene="lon"
                     /locus_tag="VF453_22290"
     CDS             7874..10303
                     /gene="lon"
                     /locus_tag="VF453_22290"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7690453.1"
                     /translation="MSGHPILPSEPITLPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     AAMESGRQIMLVAQKAAGKDEPRADDMFEIGCVSSILQMLKLPDGTVKVLVEGIQRAS
                     TREISDNGEHFVAESVPVAPQSETSPEVEALRRAVTQQFDQYVKLNKKIPPEILTSIA
                     GIDDPGRLADTIAAHLPLKLEAKQSVLDLFDIAQRLEKLLELLEHEVDILQVEKRIRG
                     RVKRQMEKSQREYYLNEQVKAIQKELGDGEEGADLEELEKKIKAARMPKEARKKADGE
                     LKKLKLMSPMSAEATVVRNYLDTILGLPWNKKTKVKHDLAHAADVLNEDHYGLEKVKD
                     RILEYLAVQQRVDKVKAPILCLVGPPGVGKTSLGQSVARATGRKFVRMALGGVRDEAE
                     IRGHRRTYIGSMPGKVLQNLTKVGTRNPLFLLDEIDKLGMDFRGDPSSALLEVLDPEQ
                     NHTFSDHYIELDFDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKLNIAQRYL
                     LPKQAKNNGVKEDELELTEGAIRGIIRYYTREAGVRSLEREVSKVCRKVVKGVQLKTI
                     IGKAVVTEENLNDYLGVRKYDFGRAEKKNQVGQVVGLAWTEVGGDLLTIESAIMPGKG
                     NIIRTGSLGDVMKESVEAARSVVRARAARLGIKDELFEKRDIHIHVPDGATPKDGPSA
                     GAAMTTAFVSALTGIPVKANVAMTGEITLRGEVTAIGGLKEKLLAAHRGGITTVMIPE
                     ENVKDLQDIPENVKGQIEIVPVRWIDKVLEVALETLPTPLPDEEAAGKDGAAVVETPV
                     VKAEVSDSLKH"
     gene            10456..10531
                     /locus_tag="VF453_22295"
     tRNA            10456..10531
                     /locus_tag="VF453_22295"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10489..10491,aa:Gly,seq:gcc)
     gene            10617..10690
                     /locus_tag="VF453_22300"
     tRNA            10617..10690
                     /locus_tag="VF453_22300"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10649..10651,aa:Cys,seq:gca)
     gene            10702..10777
                     /locus_tag="VF453_22305"
     tRNA            10702..10777
                     /locus_tag="VF453_22305"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10735..10737,aa:Gly,seq:gcc)
     gene            10976..13246
                     /locus_tag="VF453_22310"
     CDS             10976..13246
                     /locus_tag="VF453_22310"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEX7690454.1"
                     /translation="MIETTQRPPGARLTALVAGLVLAGASCGASGAGTTDYRIGSAPG
                     WVHPQAWTGPPADGVSGPGVAYGGRYDLVDDQTRLLPAGAASYHHVVVEAVTEKGVDD
                     LSHHEIEFDPGYQTLTLNQLDIVRDGRRVSRLRSVKARVLQRERDLESRVYDGRSTVD
                     LDLDDVRPGDVLEFAYTLEGQNPVFGSHHAGGADMQWRVPVMHLYRSISWPVDMPVRV
                     RSGKGAAAPVESSADGWETRAWDARDVPALQREAGTPRWYDPYAEVEWSDYADWADVG
                     RWAQALFRVPTTLGPELEAVVARIEKQDANADARVMDVLRLVQKQVRYLGIEVGSGSH
                     LPSPPDVVYARRFGDCKEKALLMAVLLRAMGISAAPALVDTDARHASADELPSHVRFD
                     HVIVRVRLDGRDWWLDPTRAPQGGTLANVVQSSYGRALVLDGSGAGLATMPEAGPGIE
                     ERRVNVDIDGSAGADKPASFTVSTLYLAQAADALRDQLGDGERGELQRRYVNFYAGSY
                     PGIRVAAPMEIADDPVANTVRVTEHYAIDDFWHGDGDDRGRRARFDANDLDAMLKRPD
                     EPIRTMPLASPGPMRTTLHLHARLPQSWDDAQTTHSVDDPAFRFRKTTRIHGNEFDVD
                     YLVELESDHVEAALMRAYVAHIERARSQLGDRLDAADAPLPVAVPSEDVAGTNGRRAM
                     AIGGAATALLAVLVAWGALRARAASRRQSARQGDASSLHGPTTVPDVIDAVGAADVLA
                     PRALAHESTTDGRPLS"
     gene            complement(13376..>14284)
                     /locus_tag="VF453_22315"
     CDS             complement(13376..>14284)
                     /locus_tag="VF453_22315"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEX7690455.1"
                     /translation="AALLAATPLAHAWGDEGHEVVAFVARAHLTPRTAQAVDRLLATD
                     TNGLKMRDGGLTTDSFARQATWADYYRDAQRVAGATPEQIHSYSWHFVDIELQGGSLD
                     KACFGFPSAGGQAASQGPDPDCVVNKIEQFAAELASPDVPDAEKLLALKFVMHFVGDL
                     HQPLHASDNLDRGGNAEMATVAGGTPMPLHAHWDTTFVTLIGAPAGGRNTDPAAVVAA
                     LRTPSDTEKAQWLGPPNPRYWAYESYALAQGYAYGGLPRPVASGAASVYKLDDQYVKR
                     ATTVVSDQLEKAGYRLAAVLNAAFDR"
BASE COUNT         2365 a         4913 c         4960 g         2046 t
ORIGIN      
        1 cggccgctgc aggccgtggt gcgcgaggtg gccgcggccg ccgatcccgc cacgcgcacc
       61 ttccaggtca aggccggcct gccggcgggt gccgcggagc tcgggcagac cgccacggtc
      121 cacgtcgagc tcgccccggc cgacggcaag ctgcgcctgc cgctgcaggc cgtgacgggc
      181 gagggcggcc gcagccaggt ctgggtggtc gacaaggcca cgatgacggt gcgcgagcag
      241 ccggtgaagg tgctccggcc cgagggcgac agcctggtcc tggacggcgg gctgaaggcg
      301 ggcgacatcg tggtcacggc cggcgcgcac gtgctcacgc cgggccagca ggtcgccttc
      361 tacgtcgaac ccgccaagcg ctgaggtccg catgagccac gatacccctg gcggcatgac
      421 gagccgcttc aacatctcgc gctgggcgct gcagcatccc gagctgacgc gctacctgat
      481 gatcgtgctg ctggtgctcg gcctggcggc ctacttccag ctcgggcagg acgaggatcc
      541 gccgttcacc ttccgcgcga tggtcgtcca ggtctactgg ccgggcgcga gcgccaagga
      601 gatggccgag caggtcaccg acaagatcga gcgcacgctg caggaggtgc cgtactccga
      661 caagatccgc agctacacca agcccggcga gtcgctgacc atcctgcagc tcaaggactc
      721 gtcgccgccg cacgaggtcg ccaacacctg gtaccaggcg cgcaagaaga tcggcgacat
      781 gcgcggcacc ctgccgcagg gcgtggccgg gccgttcttc aacgacgaat tcggcgatgt
      841 ctacggttcg atcttcgcgc tctcggccga cggcttctcc gacgaggagc tgcgcgttcg
      901 cgccgaggac atccgccagc agctgctgcg cgtgcccgac gtcgccaagg tgcagctgtt
      961 cggcgtgcaa tcgcagaagg tgttcgtcga gctgtcgcag aagcgggcgg cggagatggg
     1021 catcgacctg aaccaggtgg tctcgcagct caacgcgcag aacgtcgtcg cgggcacggg
     1081 cgtcgtcgac gccggcgcgc agaacgtgca ggtgcgcctg tccggagcgt tccagtcggt
     1141 cgacgcgatc cgtgcgttcc cgatccgcgc gaccaatccc gcgacgggcc agtccaccac
     1201 cgtgcgcctg ggcgacatcg ccgatgtcaa gcgcggctac tcggacccgc cggacgtcga
     1261 ggtgcgcgcc aacggcaagc aggtcgtcgc cctgggcgtg tcgatggcca agggtggcga
     1321 catcatctcc ctcggcaagg cgctgcagtc gcaggtcggc gagatccgca ggcagctgcc
     1381 cgtgggcatg gagatcacgc aggtgcagga ccagccgcgc gcggtcgcgc gctcggtcgg
     1441 cgagttcgtg cgcgtgctgg tcgaggcgat cgtgatcgtg ctggtcgtga gcttcgtgag
     1501 cctcgggctg cacttcaacc cgatccggat cgacatctgg cccgggctgg tcgtcgcgat
     1561 cacgatccct ctggtgctcg cgatcacctt cgtgacgatg ttctactggg gcgtcgggct
     1621 gcacaagatc tcgctgggct cgctgatcat cgcgctgggc ctgctcgtcg acgacgccat
     1681 catcgcggtc gagatgatgg tgcgcaagct cgaggagggc tactcgaagg agcgcgccgc
     1741 caccttcgcg tacgaggtga cgtcgatgcc gatgctcacc ggcacgctga tcacggccgc
     1801 gggcttcctg ccgatcgggc tggccaagtc gatgacgggc gagtacacgt tcgccatctt
     1861 cgcggtgacc tcggcggcgc tcgtgatctc gtggatggtc tcggtctact tcgtgccgta
     1921 cctcggcgcg aagctgctcc atacgcgcgc gaagccgcac gccggcgacg gcgtgcccga
     1981 ggagccgcac gagaccttcg attcgccgtt ctacggccgc ttccgcgcgc tggtcgcctg
     2041 gtgcctgcgc catcgctgga tcacgatcgg cgcgaccatc ggcacgttcg tgctgggcat
     2101 cgtcggcatg ggcctcgtgc agcagcagtt cttcccggac tcgagccggc cggaggtgat
     2161 ggtcgacctc tggctgccgg aaggcgcgac gatccgccag agcgaggccg tcgccaagcg
     2221 cttcgaggcc cgcatgatga aggagcccgg cgtgacgacg gtcacgacct gggtcggcag
     2281 cggcgtgccg cgcttctacc tcccgctcga ccagatcttc ccgcagagca atgtcagcga
     2341 agtcatcatc gagcccaccg acctcaagac ccgcgaggcg ctgcgcaaga agctgcccgc
     2401 gctgctggcc gccgagttcc cggaggtgcg cacgcgcgtg aagctgctgc ccaacgggcc
     2461 gccggtgccc tacccggtgc agttccgcgt gatggggcag gacgtcgagc aggtgcgcca
     2521 ctggggcgac ctcgccaagg acgtgctgcg cgccaatgcc cacatgcgcg gcgtcaacga
     2581 caactgggac gagaacgtgc tcgcgacgcg gctcgagatc gaccaggaca aggcccgcgc
     2641 gctcggggtg agcacgcagt cgatcgcgca ggcgacgcag acccagttct ccggctcgac
     2701 gatcggccag taccgcgaag gcgaccagct catcgacatc gtgctgcgcc agcctgcagc
     2761 cgagcgcggc aaggtgaccg acctctcgag cgcctacgtg accgccgcca acggcagcgc
     2821 ggtgccgctc gcgcaggtgg cgcacctggc gctcggctgg gagcccggcg tgctgtggcg
     2881 cgagggccgc gactacgcgg tgacggtgca gggcgacgtg gtcgagggca tgcagggccc
     2941 gaccgtgacg gccgagctgt ggccggcgct caaggcgctg caggcgaaga tgccgccggg
     3001 ctaccagatc caggtggcgg gcgcggtcga ggagtcgtcc aaggggcagg gcgccatcat
     3061 ggccggcatg ccggtgatgc tgttcatcat cttcacgctg ctgatgctgc agctgcacag
     3121 cttctcgcgc gcggtgctgg tcttcctgac cggcccgatg ggcatcgccg gcgccgccgg
     3181 cgcgctgctg gcgctgaacc ggccgttcgg cttcgtggcg atgctgggcg tgatcgcgct
     3241 gatgggcatg atcatgcgca actcggtcat cctgatcgac cagatcgagc aggagcgcgc
     3301 ccgcggcgtg ccggcatgga acgcggtgat cgagtcggtg gtgcgccgct tccggcccat
     3361 cgtgctgacc gctgcggccg ccgtgctggc gatgatcccg ctgtccgaaa gcgagttctg
     3421 gggcccgatg gcggtggcca tcatgggcgg actcgtcatc gcgacggcgc tcacgctgct
     3481 ggcgctgccg gcgatgtacg cggcctggtt ccgcgtgcga gaggacgtcc ccgcgccgtc
     3541 ggccgatgcc gcggcccagc accgcgaggg tgccaggacg ctggccagtc ccgccggagc
     3601 ctgaccggcg ccggccctgc cgctcgtccg tgccctggag gcccggggga gcggcagatc
     3661 cggcttggcc ggtctgcacg tctgcgggcc gcaggcgacc ggcgctcgcc aggcgatggt
     3721 ccgtccaccg gccagcctgt gtccgggctc acctcctcga gggcagggag cacccgcgac
     3781 cgtagggcta taatccccgg ttcaccggaa agccgggggt gcgtttcatc tctgtcccca
     3841 gcctctcggg tcacgaagcg cgggtggcga aattggtaga cgcaccaggt ttaggtcctg
     3901 acgccttcac cggcgtgcgg gttcgagtcc cgccccgcgc accagagttt gcgaaccaag
     3961 agtcccatca tggcagttaa cgtagaaacc ctcgacaagc tcgaacgccg catctccctg
     4021 accctcgccg cggacgaggt caacaaggag gtcgaagcgc gcctgaagaa gctgtcgcgc
     4081 accgtgaagg ccgacggctt ccgccccggc aaggtgccga tgtcggtcgt ggcgcagcgc
     4141 tacggctatt cggtgcagta cgaagtgatg aacgacaagc tgggcgccgc gttcgccgcc
     4201 gccgccaacg aggccaagct gcgcgtcgcc ggccagccgc gcatcgccga gaaggcgcag
     4261 agcgccgagg gcgcgatgga attcgacgcc acgttcgagg tctaccccga ggtggtgatc
     4321 ggcgacctgt cgcaggtcga gatcgagcgc gtgtcgaccg aggtcgacga ggcggccatc
     4381 gacaagacgg tggacatcct gcgcaagcag cgccgcacct ttgcccagcg cccggccgcc
     4441 gaaggcgccg aggccggcga ccgcgtgacg atcgacttcg aaggcaagat cgacggcacg
     4501 ccgttcgacg gcggcaaggc cgagaacttc cagttcatgg tgggcgaagg ccagatgctc
     4561 gagcagttcg accaggccgt gcgcggcatg aaggtgggcg agtccaagac cttcccgctg
     4621 cagttcccgg cggactacca cggcaaggaa gtcgccggtc gcgaggccga cttccttgtc
     4681 acgatgaaga agatcgaggc gcagcacctg ccggaggtcg acgacgcgtt cgcgaagtcg
     4741 ctgggcatcg ccgaaggcac ggtcgagggc ctgcgcgccg acgtccggaa gaacctcgag
     4801 cgcgaggtca agttccgcgt gctggcgcgc aacaaggccg cggcgatgga cgccctggcg
     4861 aacgccgcca cgctcgacct gcccaaggcg ctggtcgagg gcgagaccga acgcctcgtc
     4921 cagggggccc gcgaggacct gaagcagcgc ggcgtcaagg atgccgacaa ggccccgatc
     4981 ccggccgaga tcttcacgcc ccaggccgag cgccgcgtgc gcctgggcct cgtggtggcc
     5041 gagctcgtgc gcgcccagaa cctgcaggcc aagccggagc agctgcaggc gcacatcgag
     5101 gagatgtcgc agagctacga gaagccggtg gaagtccagc gctggtacct ctccgaccgc
     5161 agccgcatgg ccgaggtcga ggcgatcgtc gtcgagaaca acgtcaccga ctacgtgctc
     5221 ggccaggcca aggtcaccga caaggtgctg cccttcgacc agctgatgac ggcctgacgc
     5281 gccgtccgca cccggccgct tgacggcggt cgcggggcgc gagagcgatg ccggacgccc
     5341 gccgggacgc aggttccgcc gggcgttcgc gtttccgggg cgcccagccc cgagcgcggg
     5401 cggcgggctt gcaatgccgg gttcgggcgc catctcgccc tttggggtcg cgcgcgacgc
     5461 gacataatgg tccttcaccg acccaacaag ccacggaaga agaattcaag ccatgagcgc
     5521 acttgaaacg caagccctgg gcatggtgcc catggtcatc gagcagtccg gccgcggcga
     5581 gcgggcctac gacatctact cgcgcctgct gcgcgagcgc atcgtcttcc tggtcgggcc
     5641 ggtcaacgac cagtcggcca acctggtcgt cgcccagctg ctgttcctcg agagcgagaa
     5701 cccggacaag gacatccacc tctacatcaa ctcgcccggc ggcagcgtca gcgcggggat
     5761 gtccatcttc gacacgatga acttcatcaa gccgcacgtg tcgacgctgt gcatcgggct
     5821 ggcggccagc atgggcgcgt tcctgctggc ggcgggcgag aagggcaagc ggctgtcgct
     5881 gcccaactcg aagatcatga tccaccagcc gctcggcggc gcgcagggcc aggcgacgga
     5941 catcgagatc cacgcccgcg agatcctgaa gacccgcgag cagctcaacc gcatcctcgc
     6001 cgagcgcacc ggccagccgc tggagaagat ccaggccgac acggagcgcg actacttcat
     6061 gtccgccgac gacgccaaga gctacggcct ggtcgaccag gtcgtcgcca accgggactg
     6121 aaagcccccg acggcttccc gacccgcgag ccgcttggtc tatcattggg ccgccccctc
     6181 tcatcaccca cttttcccca caggccaaca gcaagtccat ggcagacaag aagggctcct
     6241 ccgccgaaaa ggtgctgtac tgctcgttct gcggcaagag ccagcacgag gtcaagaagc
     6301 tgatcgccgg tccgtcggtg ttcatctgcg acgagtgcat cgagctgtgc aacgacatca
     6361 tccgcgacga ggtgccggct gaaggcccgg cggcgaagtc ggccaagtcc gacctgccga
     6421 tgccgtccga gatcaaggcg agcctcgaca actacgtgat cggccaggac gtcgccaagc
     6481 gcacgctgtc ggtagccgtc tacaaccact acaagcggct caagcacatg tcgacctcca
     6541 aggacgacgt ggagctgagc aagagcaaca tcctgctcat cgggccgacc ggctccggca
     6601 agacgctgct ggcccagacc ctcgcgcgcc tgctcaacgt gccgttcgtg atcgccgacg
     6661 ccaccacgct gaccgaagcc ggctacgtgg gcgaggacgt cgagaacatc atccagaagc
     6721 tgctgcagaa ctgcaactac gacgtcgagc gggcgcagcg cggcatcgtc tacatcgacg
     6781 agatcgacaa gatctcgcgc aaggccgaca acccgtcgat cacgcgcgac gtctcgggcg
     6841 aaggcgtgca gcaggcgctg ctcaagctcg tcgagggaac gatggcgtcg gtgccgccgc
     6901 agggcggccg caagcacccg aaccaggatt tcctgcagat cgacacgacc aacatcctgt
     6961 tcatctgcgg cggcgcgttc gacggcctcg agaaggtgat ccgcaaccgc accgagcgct
     7021 cgggcatcgg cttcggcgcg accgtgcaca gcaaggacga gcgccgcgtc tcggagctgt
     7081 tccgcgaggt cgagcccgag gacctgatca agttcggcct gatccccgag ctcgtcggcc
     7141 ggctgccggt ggtcgcgacc ctgggcgagc tgaccgagga agcgctggta cagatcctca
     7201 ccgagccgaa gaacgcgctg gtcaagcagt accagaagct gttcatgatg gacggggtgg
     7261 agctggagat ccgcccgtcc gcgctcgccg cgatcgcccg caaggcgctg gcccgcaaga
     7321 ccggcgcccg tggcctgcgc tcgatcatgg agcaggcgct gatcgacacg atgttcgacc
     7381 tgccgctgct cgacggggtg gcgcaggtgg tcatcgacga gcacatcatc gaagagaacg
     7441 cgaagccgct gctggtctat cgcgagcagg cgaaggcgag cgcctgaggc ggcgccgctt
     7501 gccgagctcg ctcgtcgaac gaccgtcgac gtcgcgccgg gacgcgcctg ggcgccgtcc
     7561 ggcatcgagg aagagaggtt cgctgcgcat gcccggccat cctgtccagc cgcctgccgg
     7621 gggatccgct accggtggca tgcccgatgc cgtttccgcg gataccttcc cgcacgacgt
     7681 gccgcgcgtc gacgcgcgac aatcgccccc ggacgcccgc ctcgcgcggc ggacgggaaa
     7741 actccgagaa cgggcggagg tacggcgctg atcccgctgc agggccgggc ggattgcctc
     7801 accatatcga tcggcatctt gtaattggac gctcgcgccg catctgcggc cgagaaagag
     7861 aggttccccc cacatgtctg gacaccccat cctgccttcc gaaccgatca cgctgccgct
     7921 gctgccgctg cgcgacgtcg tcgtgttccc gcacatggtg atcccgctgt tcgtcggccg
     7981 tcccaagtcc atcaaggccc tggaagccgc gatggagtcc ggccgccaga tcatgctggt
     8041 cgcccagaag gccgccggca aggacgagcc ccgtgccgac gacatgttcg agatcggttg
     8101 cgtctcgagc atcctgcaga tgctgaagct gcccgacggc accgtgaagg tgctggtcga
     8161 aggcatccag cgcgcctcga cgcgcgagat cagcgacaac ggcgagcact tcgtcgccga
     8221 gtcggtgccc gtcgcgccgc agtcggagac ctcgcccgag gtcgaggcac tgcgccgcgc
     8281 cgtcacgcag cagttcgacc agtacgtcaa gctcaacaag aagatcccgc cggagatcct
     8341 gacctccatc gcgggcatcg acgatccggg ccgcctggcc gacacgatcg ctgcgcacct
     8401 gccactcaag ctcgaggcca agcagtccgt cctggacctg ttcgacatcg cccagcgcct
     8461 cgagaagctg ctcgagctgc tggagcacga ggtcgacatc ctgcaggtcg agaagcgcat
     8521 ccgcgggcgc gtcaagcgcc agatggaaaa gagccagcgc gagtactacc tcaacgagca
     8581 ggtcaaggcg atccagaagg agctcggcga cggcgaggag ggtgccgacc tcgaggagct
     8641 cgagaagaag atcaaggccg cgcgcatgcc caaggaagcg cgcaagaagg ccgacggcga
     8701 gctcaagaag ctcaagctga tgtcgccgat gtcggccgag gcgacggtgg tgcgcaacta
     8761 cctcgacacc atcctcggcc tgccctggaa caagaagacc aaggtcaagc acgacctggc
     8821 ccacgccgcc gacgtcctga acgaggatca ctacggcctg gagaaggtca aggaccgcat
     8881 cctcgagtac ctcgcggttc agcagcgcgt ggacaaggtg aaggcgccga tcctgtgcct
     8941 ggtcggtccc ccgggcgttg gcaagacgtc gctcggccag tccgtcgcgc gcgcgaccgg
     9001 gcgcaagttc gtccgcatgg cgctgggcgg cgtgcgcgac gaggccgaga tccgcggcca
     9061 tcgccgcacc tacatcggct cgatgccggg caaggtgctg cagaacctga ccaaggtcgg
     9121 cacgcgcaac ccgctgttcc tgctcgacga gatcgacaag ctgggcatgg acttccgcgg
     9181 cgatccgtcg tcggcgctgc tggaggtgct cgacccggag cagaaccaca cgttcagcga
     9241 ccactacatc gagctcgact tcgacctgtc ggacgtgatg ttcgtcgcca cctcgaactc
     9301 catgaacatc ccgccggcgc tgctggaccg gatggaagtg atccgcctgt ccggctacac
     9361 cgaggacgag aagctcaaca tcgcccagcg ctacctgctg cccaagcagg ccaagaacaa
     9421 cggcgtgaag gaagacgagc tggagctcac cgagggtgcg atccgcggga tcatccgcta
     9481 ctacacccgc gaggccggcg tgcggtcgct cgagcgcgag gtgtccaagg tgtgccgcaa
     9541 ggtggtcaag ggcgtgcagc tcaagacgat catcggcaag gcggtcgtga ccgaggagaa
     9601 cctcaacgac tacctcggcg tgcgcaagta cgacttcggc cgcgccgaaa agaagaacca
     9661 ggtcggccag gtggtgggcc tcgcatggac ggaggtcggc ggcgacctgt tgaccatcga
     9721 gtcggccatc atgccgggca agggcaacat catccgcacc ggctcgctcg gcgacgtgat
     9781 gaaggagtcc gtcgaggcgg cgcgctcggt cgtgcgcgcg cgcgcggcgc gcctgggcat
     9841 caaggacgag ctcttcgaga agcgcgacat ccacatccac gtcccggacg gcgcgacccc
     9901 gaaggacggc cccagcgcgg gcgccgcgat gacgacggcc ttcgtgtcgg cactcaccgg
     9961 catcccggtg aaggccaacg tggcgatgac cggcgagatc acgctgcgcg gcgaggtcac
    10021 ggcgatcggc ggtctgaagg agaagctgct cgcggcgcat cgcggcggca tcacgacggt
    10081 catgatcccg gaggagaacg tcaaggacct ccaggacatt cccgagaacg tgaagggcca
    10141 gatcgagatc gtgcccgtgc gctggatcga caaggtgctc gaggtggccc tcgagacgct
    10201 gccaaccccg ctgcccgacg aggaggcggc gggcaaggac ggcgccgcgg tcgtcgagac
    10261 gccggtcgtg aaggccgagg tcagcgactc gctgaagcac tgaagtcgcg acccgcgcgg
    10321 acgctgcggc gtccacgggg tcgcgctcga tttttcgaga aagtgcttgc agagcagttc
    10381 aaagctggca tatactgcta gcttcagcga acagacaaga acttcagcga agcggcggtc
    10441 tggtcggtgg aaaatgcggg aatagctcag ttggtagagc gcaaccttgc caaggttgag
    10501 gtcgcgagtt cgagcctcgt ttcccgctcc agcatgttga aaaagggaag cgcgtgcttc
    10561 cctttttcgt cagaatggaa ggttcatgcc gcttctcgcg ggcggcaccg aacgacggcg
    10621 cgatagcaaa gcggttatgc aacggattgc aaatccgtct agtccagttc gactctggat
    10681 cgcgcctcca gaagaagatg cgcgggaata gctcagttgg tagagcgcaa ccttgccaag
    10741 gttgaggtcg cgagttcgag cctcgtttcc cgctccagat gcttgaaagg gactcccgcg
    10801 ggagtccctt tttgcttggg catcgcctgc ggcctcgacg cgtgaggaag tgcccgaacc
    10861 cgggtacggc cctgggcccg gcatgccggc cagggcgcga caatcggcgc ccggccgctt
    10921 cggccgaagc tcgcgtggcg ggcgcggccg gatcgcgcat ccgagggagc ccttcttgat
    10981 cgaaaccacc cagcgtccgc ccggtgcgcg cctgacggcg cttgtggccg ggctcgtcct
    11041 ggccggcgcc tcgtgcggcg cgtccggcgc gggaacgacc gactaccgca tcggctcggc
    11101 gccaggctgg gtgcatccgc aggcgtggac cgggccgccc gccgatggcg tctcggggcc
    11161 cggcgtggcc tatggcggcc gctacgacct cgtcgacgac cagacccgcc tgctgccggc
    11221 cggcgccgcc tcgtaccacc atgtcgtggt cgaggcggtc acggagaagg gcgtcgacga
    11281 cctgagccac cacgagatcg agttcgatcc cggctaccag accctgacgc tcaaccagct
    11341 cgacatcgtg cgcgacggcc ggcgcgtgtc gcggctgcgc agcgtgaagg cgcgcgtgct
    11401 gcagcgcgag cgcgacctcg agtcgcgcgt ttacgacggc cgcagcacgg tcgacctcga
    11461 cctcgatgac gtccgcccgg gcgatgtcct cgagttcgcc tacacgctcg agggccagaa
    11521 cccggtgttc ggcagccacc atgcaggcgg cgccgacatg cagtggcgcg tgccggtgat
    11581 gcacctgtat cgcagcatct cctggcccgt cgacatgccg gtgcgcgtgc gctcgggcaa
    11641 gggcgccgcg gcaccggtag agtcgtcggc cgacggctgg gagacgcgcg cgtgggatgc
    11701 ccgcgacgtg cccgccctgc agcgcgaggc gggcacccct cgctggtacg acccctatgc
    11761 cgaggtcgaa tggtccgact atgccgactg ggccgacgtc gggcgctggg cgcaagcgct
    11821 gttccgcgtg ccgacgacgc tcgggccgga gctcgaggcc gtcgtcgcgc gcatcgagaa
    11881 gcaggatgcg aacgccgacg cgcgcgtgat ggacgtgctg cgcctcgtgc agaagcaggt
    11941 gcgctacctc ggcatcgagg tcggctccgg ctcgcacctg ccgagcccgc cggacgtcgt
    12001 ctacgcgcgc cgcttcggcg actgcaagga gaaggcgctg ctgatggcgg tgctgctgcg
    12061 cgcgatgggg atctccgcgg cccccgccct cgtcgatacc gacgcccgcc acgcctccgc
    12121 cgacgaactg ccctcgcacg tccgcttcga ccacgtcatc gtgcgcgtgc ggctggacgg
    12181 ccgcgactgg tggctcgacc cgacgcgcgc gccgcagggg ggcacgctcg ccaacgtcgt
    12241 gcagtcgtcc tacggccgcg ccctggtgct ggacggcagc ggcgcgggcc tggcgaccat
    12301 gccggaggca gggcccggca tcgaagagcg ccgcgtcaac gtcgacatcg acggctcggc
    12361 cggcgctgac aagcccgcga gcttcaccgt ctcgacgctg tacctggcgc aggccgccga
    12421 cgccctgcgc gaccagctcg gcgacggcga gcgcggcgag ctccagcgtc gctacgtcaa
    12481 cttctacgcc ggcagctacc ccggcatccg cgtcgccgcg ccgatggaga tcgccgacga
    12541 tccggtcgcc aacacggtgc gcgtgaccga gcactacgcg atcgacgact tctggcacgg
    12601 cgacggcgac gaccgcggcc gccgcgcccg cttcgatgcc aacgacctgg acgcgatgct
    12661 caagcggccc gacgagccga tccgcacgat gccgctcgca tcgccggggc cgatgcgcac
    12721 gacgctgcac ctgcatgcgc ggctgccgca gtcctgggac gacgcgcaga ccacgcacag
    12781 cgtcgacgac ccggccttcc gcttccgcaa gaccacgcgc atccatggca acgagttcga
    12841 cgtcgactac ctcgtcgagc tcgagtccga tcacgtcgag gcggcgctga tgcgcgccta
    12901 cgtcgcccac atcgagcgcg cgcgcagcca gctcggcgac cggctcgacg ccgccgacgc
    12961 gccgctgccc gtggcggtgc cgagcgagga tgtcgccgga acgaacggcc gccgcgcgat
    13021 ggcgatcggc ggcgccgcga cggcgctgct cgcggtgctc gtggcatggg gggcgctgcg
    13081 cgctcgcgcg gcgtcgcgac gccagtcggc gcgccagggg gatgcctcga gtctgcacgg
    13141 cccgaccacc gtgcccgacg tgatcgatgc ggtcggggca gccgacgtcc tggcaccgcg
    13201 ggcgctcgcc cacgagtcga cgaccgacgg acgccccttg tcctgacgct tcgcgctcgc
    13261 gaccgcgacc gcgacgccga gcgacgcacg gccgtcgtcc gggctgggcg gatcgctcag
    13321 ccctggatcg ccgtgccccg ccgacagcgg acaggccgcc ggcgtgtgca cgacgtcagc
    13381 ggtcgaaggc cgcgttgaga acggccgcga ggcggtagcc cgccttctcc agctggtccg
    13441 agacgaccgt cgtcgcgcgc ttcacgtact ggtcgtcgag cttgtagacc gacgcggcgc
    13501 cgcttgccac cggcctgggc aggccgccgt aggcgtagcc ctgggccagc gcgtacgact
    13561 cgtacgccca gtagcgcggg ttcggcggcc cgagccactg ggccttctcg gtgtcgctgg
    13621 gggtgcgtag cgcggcgacg acggcggcag ggtcggtgtt gcgcccgccc gccggggcgc
    13681 cgatcagcgt gacgaaggtc gtgtcccagt gcgcatgcag cggcatcggc gtcccgccgg
    13741 cgacggtggc catctcggcg ttgccgccgc ggtcgaggtt gtcgctcgca tgcagtggct
    13801 ggtgcaggtc gccgacgaag tgcatcacga acttcagggc gagcagcttc tcggcgtccg
    13861 gcacgtccgg cgaggcgagc tccgcggcga actgctcgat cttgttgacg acgcagtcgg
    13921 gatccggccc ttgcgacgcc gcctggccgc cggccgaggg gaatccgaag caggccttgt
    13981 cgagcgagcc accctgcagc tcgatgtcga cgaagtgcca ggagtagctg tggatctgct
    14041 ccggcgtcgc gccggcgacg cgctgggcgt cgcggtagta gtcggcccac gtcgcctggc
    14101 gcgcgaagct gtcggtcgtc aggccgccgt cgcgcatctt caggccgttc gtgtcggtgg
    14161 ccagcaggcg gtcgacggcc tgcgccgtgc gcggcgtcag gtgggcgcgc gccacgaacg
    14221 cgaccacctc gtggccctcg tcgccccagg catgcgcgag cggcgtcgcg gcgagcagcg
    14281 cggc
//
LOCUS       DASVLN010000949         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_265435, whole genome shotgun sequence.
ACCESSION   DASVLN010000949 DASVLN010000000
VERSION     DASVLN010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265435"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VF453_22320"
     CDS             <1..290
                     /locus_tag="VF453_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX7690456.1"
                     /translation="SGEGLDALREALLERAGAAHVPEGVFIARTRHVEALARTAHHLA
                     LARAHADQRDAALDLFAEELRLAHGALGEITGEFTADDLLGEIFGRFCIGK"
     gene            complement(594..1289)
                     /locus_tag="VF453_22325"
     CDS             complement(594..1289)
                     /locus_tag="VF453_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HEX7690457.1"
                     /translation="MIPSWLHGLALASLGVAIACALVIAADLLRRPQPMAIMNVVWPV
                     SALFGSVAWLAFYWRERRAAAGPHAAPRPFLVQVAKATSHCGSGCTLGDIVAESWALA
                     SPSVLVLFGWQTLFQERTFAAWGLDFAVAYLLGIVFQYWTIAPMRHLGLAAGLWAAVK
                     ADTLSLLAWQVGMYGGMAIAKFVVFRRLLGVELDARQPEFWFAMQAAMCLGFIASAPV
                     NAWLLRRGWKEAM"
     gene            1425..2666
                     /locus_tag="VF453_22330"
     CDS             1425..2666
                     /locus_tag="VF453_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX7690458.1"
                     /translation="MIDIAATLLVLTALLAYLNQRFLRLPMTIGVMAAAMVLSVTLVA
                     LDRFGIARSLDDRERAFVASIDFSSVLMQGMLSLLLFAGALHVDMAQLRRRRWQVGLL
                     AFAGTALSTAVVGVMLWAVLPWLGLPLPLLDCLLFGALISPTDPIAVIAMLKSAEAPP
                     DLEVVIAGESLFNDGVGVVIFSLLLEMALQGAAPTAAETGRLLLQEAGGGLAFGAVLG
                     LVLVYLLRTIDHYQVEALLTLAAVFGGYTLASDLHVSGPLAMVVTGLIVGNDGRAQAM
                     SDVTRHHVDLFWELLDEMLNALLFVLVGLEVVAIRFPVEGLRAAGVGAVAIAVSLLAR
                     WLTVGLPTLALGRRMRLPRGSAAILTWGGLRGGISIALALAIPASHHPQTLVALTYCV
                     VAFSVLVQGLSFGRLAKRWLA"
     gene            2764..3588
                     /locus_tag="VF453_22335"
     CDS             2764..3588
                     /locus_tag="VF453_22335"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HEX7690459.1"
                     /translation="MSKTATIAMSVLAGALAVGAAAWWLRSGGAPAPASVAQEAAGTS
                     TPPSAPTAAVPVASAPPRIAHPVPTMASASSPPARTFELELVGLLGGAPVAALFRSDD
                     FDRRFVATIDNLGRSAAPPSVWPVNPVGGRLRVAASPDGDVIGPDNGARYEPYVRLLE
                     QVDLSAVVRLYVQHYPELQREYEALGFPGRYFNDRVVAVLDQLLATPTAAGALHVHLP
                     RVEGPQPTRPWLLYDFDDPALRSLAAGQRLLLRMGPDNERRVKERLRELRRLLVTA"
     gene            3827..>4298
                     /locus_tag="VF453_22340"
     CDS             3827..>4298
                     /locus_tag="VF453_22340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690460.1"
                     /translation="MTLRTLRLQLRFLLPLVLVLVAAAYVAVPVLDGVTLRWFERDLG
                     SRGTLVANALSDSVIEALRTGHPERLRALFDRAAQDERLFAIGLCTPDGRMLERTPGF
                     PDRVDCAEAVLVSARAQPRLQLAGGAVRVSVHDILYQPPAVPVPAPSPAATASAA"
BASE COUNT          536 a         1573 c         1531 g          658 t
ORIGIN      
        1 gcagcggcga gggcctggat gcgctgcgcg aggcgctgct cgaacgcgcc ggcgcggccc
       61 acgtccccga gggcgtcttc atcgcccgca cgcgccacgt cgaggcgctc gcgcgcaccg
      121 cgcaccacct cgcgcttgcc cgcgcgcacg ccgaccagcg cgacgccgcc ctcgacctgt
      181 tcgccgagga actgcgcctg gcccacggcg cgctcggcga gatcaccggc gagttcaccg
      241 ccgacgacct gctcggcgag atcttcgggc ggttctgcat cggcaagtga gcgcggctcc
      301 ggccagatcc ccgctgggca aggcccgcag gcgccatcat gctggatgcg gatgagatgc
      361 tcaggcgcgg ggcgttcgtc ggggaagatt gcgtttggtt caacaatggc ctcgcctggg
      421 cgctgttctc tcgctgctcg gatccgctcg tggtgctggc gaacctcctg ggcttcgtgc
      481 tcgcctcccg ccatgggccg ctcgccccgg cggccgcgac ggcttcggtc aaggggcacg
      541 gctggcgtcg catcgggcgg gcccgtcccg cgccgtcccg ccgtggcccc ggcctacatc
      601 gcttccttcc atccccgccg cagcaaccac gcgttgaccg gcgcggaggc gatgaagccc
      661 aggcacatgg cggcctgcat cgcgaaccag aactcgggct ggcgcgcgtc cagctcgacg
      721 ccgagaaggc gccggaagac gacgaacttc gcgatcgcca tgccgccgta catgcccacc
      781 tgccaggcca ggagcgacaa cgtgtccgcc ttcacggccg cccacaggcc ggcggcgagc
      841 cccaggtgcc gcatcggcgc gatcgtccag tactggaaga cgatgccgag caggtaggcg
      901 accgcgaagt cgaggcccca ggccgcgaac gtcctctcct ggaacagcgt ctgccaaccg
      961 aagaggacca ggaccgaggg cgaagccagc gcccagctct cggccacgat gtcgccgagc
     1021 gtgcagccgc tgccgcagtg gctcgtcgcc ttggcgacct gcacgaggaa cggtcgcggc
     1081 gccgcgtgcg gaccggcggc cgcgcggcgc tcgcgccagt agaaggcgag ccaggccacg
     1141 ctgccgaaca gcgcggacac cggccacacg acgttcatga tcgccatcgg ctggggccgg
     1201 cgcaggaggt cggccgcgat gacgagcgcg caggcgattg ccacccccag cgacgccagc
     1261 gcgagcccat gcagccacga gggaatcatc gagcgcttcc tgttgaatcg agtgtcgaga
     1321 ggccacgccg ggcaacgtgc gtaccccgcc gagccgcgac ccatgccgac acctcgcgcg
     1381 gacaatggcg agaaccttcg cctcttcgac gacgagcaac gcagatgatc gacattgccg
     1441 ccaccctgct cgtgctcacg gcgctgctgg cctacctcaa ccagcgcttc ctccggctgc
     1501 ccatgacgat cggggtgatg gcagccgcca tggtcctgtc cgtgacgctg gtcgcgctgg
     1561 atcgcttcgg catcgcccgg tcgctggacg accgcgagcg cgccttcgtg gcgtcgatcg
     1621 acttctccag cgtgctcatg cagggcatgc tgtcgctgct gttgttcgcg ggcgcgctcc
     1681 acgtcgacat ggcgcagctg cgccgccgtc gctggcaggt gggcctgctc gcgttcgccg
     1741 ggacggcgct gtcgaccgcg gtcgtgggcg tgatgctctg ggcggtgctc ccgtggctgg
     1801 gcctgccctt gccgttgctg gactgcctgc tcttcggcgc gctgatctcg ccgacggatc
     1861 ccatcgcggt catcgcgatg ctgaagtcgg cggaggcgcc ccccgacctc gaggtcgtga
     1921 tcgccggcga gagcctgttc aacgacggcg tgggcgtcgt gatcttcagc ctgctgctgg
     1981 agatggcact gcaaggagcc gccccgaccg cggcggagac cggccggctc ctgctccagg
     2041 aagcgggcgg gggcctcgcg ttcggggcgg tgctgggcct cgtgctggtg tacctgcttc
     2101 gcaccatcga ccactaccag gtcgaggcgc tgctcacgct cgccgcggtc ttcggcggct
     2161 acacgctggc gagcgacctg cacgtctcgg gcccgctggc catggtggtg accgggctca
     2221 tcgtcggcaa cgatggccgg gcgcaggcca tgtccgacgt cacgcggcat cacgtcgacc
     2281 tcttctggga actgctcgac gagatgctga acgcgctgct gttcgtgctg gtcggcctcg
     2341 aggtcgtggc gatccgcttt cccgtggagg gcctgcgcgc cgccggcgtc ggggccgtgg
     2401 cgatcgcggt gtcgctgctg gcccgctggc tgaccgtcgg cctgccgacg ctcgcgctcg
     2461 ggcgccggat gcgcttgccg cgcggctccg ccgccatcct gacctggggc ggcctgcgcg
     2521 gcggcatctc gatcgcgctg gcgctggcga taccggccag ccaccatccg cagaccctcg
     2581 tggcgttgac ctactgcgtc gtcgcgtttt ccgtcctggt gcaggggctc agcttcggga
     2641 ggctcgcgaa gcgctggctc gcctgatccg gcccggccgc cggctccgtt cctgacggca
     2701 aatatgtttg tacaataaca tcttgtcgcg cagccatcgt ggtcgccagc tccaggaatt
     2761 catgtgtcga agactgccac catcgcgatg agcgtgcttg ccggagccct ggccgtcggc
     2821 gcggccgcgt ggtggctgag gtccggcggc gccccggcgc cggcgtccgt ggcccaggag
     2881 gccgccggca cgtccacgcc gccatcggcg cccacggcgg cggtgccggt cgcgtcggcg
     2941 ccgccgcgca tcgcccaccc ggttccgacg atggcctcgg cgtcgtcccc gccggcgcgg
     3001 accttcgagc tcgagctggt gggcctgctt ggtggcgcgc cggtggcggc gctgttccgg
     3061 tccgacgact tcgaccgccg gttcgtggcc accatcgaca acctcggccg ctcggcggcg
     3121 ccgcccagcg tgtggccggt caacccggtc ggcgggcgcc tgcgggtcgc cgcgagcccg
     3181 gatggcgacg tcatcgggcc tgacaacggc gcgcgctacg agccctacgt tcgcctgctg
     3241 gaacaggtgg acctgtccgc ggtggtccgc ctctacgtcc agcactatcc cgaactgcag
     3301 cgcgagtacg aagcgctggg atttcccggc cgctacttca acgaccgcgt cgtggcggtg
     3361 ctcgaccagc tgctcgccac gccgacggcc gcgggcgcgc tgcacgtgca cctgccccgc
     3421 gtcgaggggc cgcagccgac gcggccatgg ctcctgtacg acttcgacga tcccgcgttg
     3481 cgctcgctgg ccgcggggca gcgcctgctg ctgcgcatgg ggccggacaa cgagcggcgc
     3541 gtgaaggagc gccttcggga gttgcgccgc ctgctggtga cggcatgagg cgaggacgca
     3601 ggatgaatga gcgctgcggc cgcgccggcg atcgggcacc gggaaagcct gtccaccacg
     3661 tcatggccgg aagcggtccg gggccgaccg cgctcctgcc gaccggcctg cgtccgtcgt
     3721 tccgctcggc cgcggccgca cgtcttggga ggctgggttg acggtcggga tcgacgcgtc
     3781 gcccgggcgg cgagacctcg ctccgggcgg gtcgggcagg atggcgatga cgctgcggac
     3841 gctgcggctg caactgcgct tcctgctacc gctggtgctg gtgctcgtcg cggccgccta
     3901 tgtcgcggtg ccggtgctcg acggcgtcac cttgcgctgg ttcgagcgcg acctcggcag
     3961 ccgcggcacg ctggtggcca acgcgctgtc cgactccgtg atcgaggcat tgcgcacggg
     4021 ccatcccgaa cgcctgcggg ccctgttcga ccgcgccgca caggacgagc gcctcttcgc
     4081 gatcggcctg tgcacgcccg acggtcgcat gctggagcgc acgccgggct tcccggaccg
     4141 cgtcgactgc gccgaggccg tgctggtcag cgcgagggcg cagcctcggc tgcagcttgc
     4201 gggcggcgcc gtgcgcgtct ccgtgcacga catcctgtat cagccgcccg cggtgccggt
     4261 acccgcgccg agccccgccg cgacggcatc tgcggcgg
//
LOCUS       DASVLN010000950         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_104551, whole genome shotgun sequence.
ACCESSION   DASVLN010000950 DASVLN010000000
VERSION     DASVLN010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104551"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VF453_22345"
     CDS             complement(<1..993)
                     /locus_tag="VF453_22345"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEX7690461.1"
                     /translation="MHQHAARNRAYRLAWNEATGQWRPVPEFARTRGGRRGAVAAGLL
                     LAGLSAGAAGAAGPSPPAPTQLPTGATVTAGQVSLHGSGATLDVRQGSRVGAIDWTSF
                     DLGSAASVHFLQPDAASVTLNRVTGGDASRIFGHVDATGQVFLVNPNGVYFAPGATVD
                     VGGLVASTLGLGDEDLAAGRLRFARDPSRAAGSVVNEGTLTARPGGYVALLAPEVRNP
                     GVVTARLGGVALAAGDAVTLRMDGHALVGVSVEPAALQALVDNGGAVITPGGRVLLSA
                     RAASQLAGGVVRNTGVVEATGLASRGGRILLEASDAVDEDGTLSAVGTGGGAGGT"
     gene            complement(1005..>2536)
                     /locus_tag="VF453_22350"
     CDS             complement(1005..>2536)
                     /locus_tag="VF453_22350"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX7690462.1"
                     /translation="LLAPAPAPVRPGDDLALVVRRFRFEGNTRLDEARLAEATRPWLG
                     RPLRFADLQDAAAAVAQAYERAGWLVRVVLPRQDVTDGVVTIAVVEAVFGGLRLDGQP
                     TRVSPGRLRAVIEGAQPVGEPLSAPALDRAMRLVEELPGVRASGRLVEGREQGRTELA
                     LSAVDAPLATGRLAVDNAGAHATGRLEASAALVLASPLRLAERWQLDVSHARGDDYLH
                     AGVWLPAPGAAWRFGASASAMGYRLVESSFASLQAHGSAHTVALQAEDALVRSAAGQL
                     TLTLQAGRNRFENAALGVETSNYRVTTLSAGLDAGASDDVAGGGTSRASVVLVAGRVD
                     LAGSPNQAADAASGHAQGGFARLRFALAREQALGEGWSLAASLSGQWAGKDLDSSERM
                     SIGGPGAVRAFPSDDGAGDQALVLSAELRKRLGERAALAAFLDWGRVQVDKRTGHPGA
                     PLVEAYALGGLGVAADWSPVAGIGFRASAAQPLSPDPAPSADAPRRLAPLLTLQLDAA
                     F"
BASE COUNT          303 a         1011 c          966 g          256 t
ORIGIN      
        1 ggtgccgccg gcgccgccgc cggtgccgac ggccgacagc gtgccgtcct cgtccacggc
       61 gtcgctggcc tccagcagga tgcggccgcc gcgcgacgcg aggcccgtgg cctccacgac
      121 gcccgtgttg cgcaccacgc cgccggcaag ctggctggcg gcgcgcgccg acagcagcac
      181 ccgcccgccg ggtgtgatca ccgcgccgcc gttgtccacc agcgcctgca gcgccgccgg
      241 ctccaccgac acgccgacca gcgcatgccc gtccatccgc agcgtgacgg cgtcgcccgc
      301 ggccagcgca acgccgccca gccgcgccgt caccacgccg ggattgcgca cctcgggcgc
      361 cagcagcgcc acgtagccgc cggggcgcgc cgtgagcgtg ccctcgttga cgacgctgcc
      421 cgcggcgcgc gacggatcgc gggcgaagcg caggcggccg gcggcgaggt cctcgtcgcc
      481 caggcccagc gtgctggcca cgaggccgcc cacgtccacc gtcgcgccgg gcgcgaagta
      541 gacgccgttc gggttgacga ggaagacctg gccggtggcg tcgacgtggc cgaagatgcg
      601 gctcgcgtcg ccgcccgtca ccctgttgag cgtgacggac gcggcgtcgg gctgcaggaa
      661 gtgcacgctg gccgcgctgc cgaggtcgaa gctggtccag tcgatggcgc cgacgcgcga
      721 accctggcgc acgtcgagcg tggcgccgct gccgtgcagc gagacctggc cggcggtgac
      781 ggtcgcgccc gtgggcagct gcgtcggcgc gggcggcgac gggccggcgg cgccggccgc
      841 ccccgcggac aacccggcca acagcagccc cgccgccacg gcccccctgc ggccgccgcg
      901 cgtgcgggcg aattccggca ccggccgcca ctggccggtg gcctcgttcc aggcgagccg
      961 gtaggcgcgg ttgcgggcgg cgtgctggtg catggcccgt ctcgtcaaaa ggccgcgtcc
     1021 agctggagcg tcagcagcgg cgcgaggcgg cgcggtgcat cggcggacgg cgccggatcg
     1081 ggagacagcg gctgcgcggc gctcgcccgg aacccgatcc cggccaccgg cgaccagtcc
     1141 gccgccaccc ccagcccgcc cagcgcgtag gcctcgacca gcggcgcgcc gggatggccg
     1201 gtgcgcttgt cgacctgcac ccggccccag tcgaggaagg cggcgagggc ggcgcgctcg
     1261 cccaggcgct tgcgcagctc cgcgctcagc accagggcct ggtcgccggc gccgtcgtcg
     1321 gacgggaacg cccgcacggc accgggcccg ccgatcgaca tgcgctcgga cgaatcgagg
     1381 tccttgcccg cccactgccc cgacaggctg gccgccagcg accagccctc gcccagcgcc
     1441 tgctcgcgcg cgagcgcgaa gcgcagcctc gcgaagccgc cctgggcgtg cccggacgcg
     1501 gcgtcggccg cctggttggg cgagccggcg aggtcgacgc ggccggccac cagcacgacg
     1561 ctcgcgcggc tcgtgccgcc gccggcgacg tcgtcgctcg cgccggcgtc caggccggcc
     1621 gacagcgtgg tgacgcggta gttcgaggtc tcgacgccca gcgcggcgtt ctcgaatcgg
     1681 ttgcggccgg cctgcagcgt cagcgtgagc tggccggcgg cgctgcgcac gagcgcgtcc
     1741 tccgcctgca gcgcgaccgt gtgcgcgctg ccgtgcgcct gcagcgacgc gaacgaggac
     1801 tcgaccagcc ggtagcccat cgccgaggcg ctggcgccga agcgccaggc ggccccgggc
     1861 gcgggcagcc agacgccggc atgcaggtag tcgtcgccgc gcgcatgcga gacgtcgagc
     1921 tgccagcgct cggccagccg cagggggctg gccagcacca gggcggcgga ggcctcgagc
     1981 cggcccgtgg catgggcgcc ggcgttgtcc acggccaggc ggccggtggc cagcggcgcg
     2041 tcgaccgcgc tcagcgcgag ctcggtgcgc ccctgctcgc ggccttcgac gaggcgcccg
     2101 ctcgcccgca cgccgggcag ttcctccacc aggcgcatcg cgcggtcgag cgccggggcg
     2161 ctcagcggct cgcccacggg ctgggcaccc tcgatcacgg cgcgcaaccg tccgggcgac
     2221 acgcgcgtgg gctgcccgtc cagccgcagt ccgccgaaga ccgcctcgac gacggcgatg
     2281 gtcaccacgc cgtcggtcac gtcctggcgc ggcaggacga cccgcaccag ccagccggcg
     2341 cgctcgtagg cctgggccac cgcggcagcg gcgtcctgta ggtcggcgaa acgcagcggc
     2401 cggccaagcc agggccgcgt cgcctccgcc aggcgcgcct cgtccaggcg tgtgttgccc
     2461 tcgaagcgga agcggcggac gaccagcgcg agatcgtcgc ccgggcgcac cggcgcgggc
     2521 gccggcgcca gcaacg
//
LOCUS       DASVLN010000951         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_104624, whole genome shotgun sequence.
ACCESSION   DASVLN010000951 DASVLN010000000
VERSION     DASVLN010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104624"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /gene="atpA"
                     /locus_tag="VF453_22355"
     CDS             <1..1506
                     /gene="atpA"
                     /locus_tag="VF453_22355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7690463.1"
                     /translation="EGLGASSDIRNQGTVVSVTDGIVRVHGLSDAMQGEMLEFPAAPN
                     GQPTFGLALNLERDSVGAVILGEYEHISEGDTVKCTGRILEVPVGPELIGRVVNALGQ
                     PIDGKGPINAKLTDVIEKVAPGVIARKSVDQPVQTGLKSIDSMVPIGRGQRELIIGDR
                     QTGKTAVAIDTIINQKGQNMTCVYVAIGQKASSIKNVVRALEANGAMAYTIVVAASSS
                     ESAAMQYVSAYSGCTMGEYFRDRGQDALIIYDDLSKQAVAYRQVSLLLRRPPGREAFP
                     GDVFYLHSRLLERAARVNADYVEQFTKGEVKGKTGSLTALPIIETQAGDVSAFVPTNV
                     ISITDGQIFLETNLFNAGIRPAINAGISVSRVGSSAQTKLIKGLSGGIRTDLAQYREL
                     AAFAQFASDLDEATRKQLDRGARVTELLKQAQYSPLPISLMAASLYAANKGHMDSLEI
                     KQVLPFEHGLHQHLKSNHSALLAKLESDKAMDKAAEEELSNAIVAFKKSFA"
     gene            1546..2436
                     /gene="atpG"
                     /locus_tag="VF453_22360"
     CDS             1546..2436
                     /gene="atpG"
                     /locus_tag="VF453_22360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX7690464.1"
                     /translation="MAVGKEIRGKIKSVENTRKITKAMEMVAASKMRKAQDRMRAARP
                     YSERIAGITANLSKANPEYKSPYMRDTSGSGKAGNVGFIVVTTDKGLCGGLNTNVLRA
                     VTQKMRDVQAAGGKVSAVAIGNKGLGFLNRSGAQVVSHVTQLGDSPSLEKLIGPIKVM
                     LEMFIEGKLDAVHLCYTKFINTMKQEPMVVPLLPLSAEGMAASQSVQQASHAWDYIYE
                     PDASVVIDELLKRYIEALVYQAVAENMASEQSARMVAMKAATDNAGNLIGELKLVYNK
                     TRQAAITKELSEIVGGAAAV"
     gene            2464..>3243
                     /locus_tag="VF453_22365"
     CDS             2464..>3243
                     /locus_tag="VF453_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7690465.1"
                     /translation="MTTSTAQGKIVQTIGAVVDVEFPRDQMPRVYDALKMDGTSLTLE
                     VQQQLGDGVVRTIALGSSDGLRRGAMVRNTGAPITVPVGKATLGRIMNVLGEPIDERG
                     PVSDELTASIHRKPPAYDELSPSQELLETGIKVIDLICPFAKGGKVGLFGGAGVGKTV
                     NMMELINNIAKAHSGLSVFAGVGERTREGNDFYHEMSDSNVVVQDNLSESKVAMVYGQ
                     MNEPPGNRLRVALTGLTIAESFRDEGRDVLFFVDNIYRYTLA"
BASE COUNT          619 a         1100 c         1051 g          473 t
ORIGIN      
        1 gaaggcctgg gggcgtcgtc cgacatccgc aaccagggca cggtcgtctc ggtcaccgac
       61 ggcatcgtcc gcgtccacgg cctgtccgac gccatgcagg gcgaaatgct ggagttcccg
      121 gcagccccga acggccagcc gaccttcggc ctcgcgctga acctcgagcg cgactccgtc
      181 ggcgccgtca tcctgggcga gtacgagcac atctccgaag gcgacaccgt caagtgcacg
      241 ggccgcatcc tggaagtgcc cgtcggcccc gagctgatcg gccgtgtggt caacgcgctg
      301 ggccagccga tcgacggcaa gggcccgatc aacgccaagc tgacggacgt catcgagaag
      361 gtcgccccgg gcgtgatcgc ccgcaagtcc gtcgaccagc cggtgcagac cggcctgaag
      421 tcgatcgact cgatggtgcc gatcggccgc ggccagcgcg aactgatcat cggcgaccgc
      481 cagaccggca agaccgcggt ggcgatcgac acgatcatca accagaaggg tcagaacatg
      541 acctgcgtct acgtcgccat cggccagaag gcgtcgtcga tcaagaacgt cgtgcgcgcc
      601 ctggaggcca atggcgcgat ggcctacacc atcgtggtcg ccgcttcgtc gtcggaatcg
      661 gccgcgatgc agtacgtgtc ggcctactcc ggctgcacga tgggcgagta cttccgcgac
      721 cgcggccagg atgccctgat catctatgac gacctgtcca agcaggccgt cgcgtaccgc
      781 caggtctcgc tgctgctgcg ccgcccgccg ggccgcgaag ccttccccgg cgacgtgttc
      841 tatctccaca gccgcctgct cgagcgcgcc gcccgcgtga acgccgacta cgtcgagcag
      901 ttcacgaagg gtgaggtcaa gggcaagacc ggttcgctga ccgcgctgcc gatcatcgag
      961 acgcaggccg gcgacgtgtc ggcgttcgtc ccgacgaacg tcatctcgat cacggacggc
     1021 cagatcttcc tggaaaccaa cctgttcaac gccggcatcc gtcccgccat caacgcgggt
     1081 atctcggtgt cgcgcgtcgg ctcgtcggcc cagaccaagc tgatcaaggg cctgtccggc
     1141 ggcatccgta ccgacctggc gcagtaccgc gaactcgcgg ccttcgccca gttcgcgtcg
     1201 gacctggacg aagccacccg caagcagctc gaccgcggcg cccgcgtcac ggaactgctg
     1261 aagcaggcgc agtactcgcc gctgccgatc agcctgatgg ccgcgtcgct gtacgccgcc
     1321 aacaagggcc acatggactc gctggagatc aagcaggtgc tgccgttcga gcatggcctg
     1381 caccagcacc tgaagtccaa ccactcggcc ctgctggcca agctggagtc ggacaaggcc
     1441 atggacaagg cggccgagga agaactctcc aacgcgatcg tcgcgttcaa gaagtccttc
     1501 gcctgatccc gccgaccacg accctctttc ccaaggagaa ccgtcatggc ggtaggcaag
     1561 gaaatacgcg gcaagatcaa gtcggtggaa aacacccgca agatcacgaa ggcgatggag
     1621 atggtcgccg cgtccaagat gcgcaaggcg caggaccgca tgcgcgcggc ccggccctac
     1681 agcgaacgca tcgccggcat cacggccaac ctgtccaagg cgaacccgga gtacaagtcg
     1741 ccctacatgc gcgacacctc cggctcgggc aaggccggca acgtcggctt catcgtggtg
     1801 acgacggaca agggcctgtg cggcggcctg aacaccaacg tgctgcgcgc cgtcacgcag
     1861 aagatgcgtg acgtccaggc cgcgggcggc aaggtgagcg cggtcgcgat cggcaacaag
     1921 ggcctcggct tcctgaaccg ctccggcgcg caggtggtca gccacgtgac gcagctgggc
     1981 gactcgccgt cgctcgagaa gctgatcggg ccgatcaagg tcatgctgga gatgttcatc
     2041 gagggcaagc tggacgccgt ccacctctgc tacacgaagt tcatcaacac gatgaagcag
     2101 gagccgatgg tcgtgccgct gctgccgctg tcggccgaag gcatggccgc cagccagagc
     2161 gtccagcagg cctcgcacgc ctgggactac atctacgagc cggacgcgtc ggtcgtgatc
     2221 gacgagctgc tcaagcgcta catcgaggcg ctggtgtatc aggcggtcgc cgagaacatg
     2281 gcgtcggagc agtccgcgcg aatggtggcg atgaaggccg ccacggacaa cgccggcaac
     2341 ctcatcggcg agctcaagct ggtctacaac aagacgcgtc aggcggcgat cacgaaggaa
     2401 ctgagtgaga tcgtcggcgg tgccgctgcg gtttaaacgt ttgcaagatt caaggaacga
     2461 aagatgacga cctctactgc ccagggcaag atcgttcaaa ccatcggcgc cgtggtcgac
     2521 gtggaattcc cgcgcgacca gatgccgcgc gtctacgacg ccctgaagat ggacggcacc
     2581 tcgctgacgc tggaagtcca gcagcagctg ggcgacggcg tggtgcgcac catcgcgctg
     2641 ggttcctcgg acggcctgcg ccgcggcgcc atggtccgca acacgggcgc cccgatcacc
     2701 gtgccggtcg gcaaggcgac gctgggccgc atcatgaacg tgctcggcga gcccatcgac
     2761 gagcgcggcc cggtcagcga cgagctgacg gcctccatcc accgcaagcc gcccgcgtac
     2821 gacgagctgt cgccgtcgca ggagctgctc gagacgggca tcaaggtcat cgacctgatc
     2881 tgcccgttcg ccaagggcgg caaggtcggc ctgttcggtg gcgccggcgt cggcaagacc
     2941 gtcaacatga tggagctgat caacaacatc gcgaaggcgc actcgggcct gtccgtgttc
     3001 gccggcgtgg gcgagcgcac ccgcgagggc aacgacttct accacgagat gtcggactcc
     3061 aacgtcgtcg tgcaggacaa cctgtccgag tcgaaggtcg cgatggtcta cggccagatg
     3121 aacgagccgc cgggcaaccg cctgcgcgtc gcgctgaccg gcctgacgat cgccgagtcg
     3181 ttccgcgacg aaggccgcga cgtgctgttc ttcgtggaca acatctaccg ctacacgctg
     3241 gcc
//
LOCUS       DASVLN010000952         6449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_265502, whole genome shotgun sequence.
ACCESSION   DASVLN010000952 DASVLN010000000
VERSION     DASVLN010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265502"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VF453_22370"
     CDS             <1..403
                     /locus_tag="VF453_22370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690466.1"
                     /translation="PASAAPAVGANASGGAPAGFVITGSVTAARSKIDLSQPIAPPRL
                     QEQVVLSAGAGAGADAGPPSEGIPAEALRRVLKVLSAHLGPIAGDVLKHVAGRARTIP
                     ELHKLLLEQAGAGVDKKRMAKQLKAVAKLPL"
     gene            complement(464..907)
                     /locus_tag="VF453_22375"
     CDS             complement(464..907)
                     /locus_tag="VF453_22375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEX7690467.1"
                     /translation="MSSRDAEPIVERPPYQKYDVLVRPSLIDGHGVFAAEFVPPHAKI
                     GEIRGEAISCAEGRRRAARLHRIMIVEISGKTAIDASRSTDPMRFTNHSCQANARLAI
                     NAGRIEFYSLRPIAPGEEITVNYGRTHHEGKLRCRCGAPGCVGAL"
     gene            complement(924..1361)
                     /gene="queF"
                     /locus_tag="VF453_22380"
     CDS             complement(924..1361)
                     /gene="queF"
                     /locus_tag="VF453_22380"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX7690468.1"
                     /translation="MPPNPPTAPSKELHVFANPASGRDYVIQFQIPEFTCHCPLTGQP
                     DFAHFTIDMICDELCIELKSLKMYMWSYRNEGSFHEAVTNTILEDIVAAADPRFVRIT
                     AKWFVRGGIYTNVVVEHRKRGWTPAPRVDLPAVASLGTATGLL"
     gene            1775..5293
                     /gene="smc"
                     /locus_tag="VF453_22385"
     CDS             1775..5293
                     /gene="smc"
                     /locus_tag="VF453_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX7690469.1"
                     /translation="MRLNSIKLSGFKSFADPTNFQLPGQRVGVVGPNGCGKSNIMDAV
                     RWVLGESKASELRGESMQDVIFNGSGSRKPAGRASVELVFNNEAARAGGPWNQFSEIA
                     VKRVLTREGASSYYINNQPVRRRDVQDVFLGTGLGPRAYAIIGQGTISRIIESRPEEL
                     RLFLEEAAGVSKYKERRRETENRLKDTRDNLLRVEDILRELNANLEKLERQAEVATKY
                     RTLQEQGTLKLHQLWFLKHRDAAAEGERVKLAGLEATNALEARLAELRHVEAQLEEVR
                     QQHYGASDRLHGAQGELAQAQLEVSRLEERIRYVVEGRQRAESRLADLKSQNAQWTDR
                     QAQAEAELERNAEQIAGAGEQAELLAAQAEEESLRLPDFEEAVRVAQQRTTGQRGEVV
                     QVQQQIQVLAAESRSIEEQQRALRGRREKLAGERSQLATPDLQRLEELKARTAEADEL
                     KDETEARLAEVAEQVPALDEQRRAQQDVLNRDQARQADLSARLEALRALQDKVQTEGK
                     LKPWLARHGLDGLQGLWTRVHIEPGWETALESALRERLNALAVGRLEMVRSFATDAPP
                     AKLAFYSPPGAAIANTHRTLPRLSDLLRLNDTGLAALLNDWLEGVYTAASMDDALAAR
                     GQLTHGEVIMTRDGHAVSQFAVAFYAPDSEQAGMLARAQEIEHLERESRAQALITDEA
                     RAALVRLEAQYTDAAQRLQSVRREASDAQTRAHELRVELLRLSSQAEAAATRRTQLED
                     ELAEIDAQMEGLAERRASGEARFEELDMQLAVTQERQAELEEGVIQAERKLADAREQL
                     RALERQAQEAQFQTRALAARQGELQRQIETAQQQVAQNVQAGEQLTLELDAFNDQAAQ
                     TGLQDALELRAAREAALGVVRAEYDELSAQLRRADEQRMEFERSLQPLRDAITKLQLE
                     QQAADLGGAQYMEQLVAAEVDLEALARNIEEGGVKLHGLQSEIDRIGREVNALGAVNL
                     AALDELVAARERKTFLDAQNTDLNEAIRTLEGAIQKIDLETRDLLMKTFNLVNEQFGR
                     MFPSLFGGGQAKLVMTGDEILDAGVQVMAQPPGKKNSTIHLLSGGEKALTAIALVFAI
                     FMLNPAPFCLLDEVDAPLDDANTERYAKLVAEMSHKGTQFLFISHNKIAMEMAEQLIG
                     VTMQEQGVSRIVAVDMEAALSMAEAA"
     gene            5296..6354
                     /locus_tag="VF453_22390"
     CDS             5296..6354
                     /locus_tag="VF453_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7690470.1"
                     /translation="MTINLTVALAFVALLVLGGIVVQATWKTRRIAARRGPAVPTPGE
                     GPREPVLGEIVVDDPLELPTPAPARPDSRAAPLRRGVKLDALIDAIATLAVDAPVTGE
                     AALSHMPATARAGTKPFFIEGLNAETGEWEAVVAHARYGEFQAGVQMANRNGALNEIE
                     FSEFVQKLEAFAQGIGAATDLPDMLETVARARELDAFASQHDAQLVALLRANSVAWSV
                     GYLQQVAQRHGFVPGAVPGRLVLPAADEGAPPVLVLAFDAQAALADDPGHAAVREVSL
                     GLDVAQTDESDEPFAAWHGLARKLAEDLDAAIVDDQGQPITLHAFSAIHAELARLYEA
                     LAARDLAAGSAGARRLFS"
BASE COUNT          955 a         2294 c         2398 g          802 t
ORIGIN      
        1 gcccgcgtcc gccgcgccgg ccgtcggcgc gaacgcgagc ggaggcgccc ccgccgggtt
       61 cgtcatcacg ggttccgtga cggcggcgcg ttcgaagatc gacctcagcc agcccatcgc
      121 cccgccgcgc ctgcaggagc aggtcgtgct gtccgccggc gccggcgccg gcgccgacgc
      181 cgggccgccg tccgaaggca tccccgccga ggcactgcgc cgcgtgctga aggtgctctc
      241 cgcccacctc ggcccgatcg ccggcgacgt cctgaagcac gtggccggcc gcgcccgcac
      301 cattcccgag ctgcacaagc tgctgctcga acaggccggc gcgggcgtcg acaagaagcg
      361 catggccaag cagctcaagg cggtggcgaa gctgccgctc tgacagcccc tcggggcagg
      421 gcccgcgcgt cgcggcgtcc cggccggcgg cagccgcccg gcgtcagagc gccccgacgc
      481 accccggcgc cccgcaccgg cagcgcagct tgccctcatg gtgcgtcctg ccgtagttga
      541 cggtgatctc ctcgcccggc gcgatcggcc gcagggagta gaactcgatg cggccggcgt
      601 tgatcgccag ccgcgcgttg gcctggcagg agtggttcgt gaagcgcatc gggtcggtgg
      661 agcgcgaggc gtcgatcgcg gtcttgcccg agatctccac gatcatgatg cgatgcagac
      721 gcgccgcgcg ccggcggccc tcggcgcagc tgatcgcctc gccgcgaatc tcgcctatct
      781 tggcgtgcgg cggcacgaac tcggccgcga agacgccgtg gccgtcgatc aggctgggcc
      841 gcaccagcac gtcgtacttc tggtacggcg ggcgctcgac gatcggctcg gcgtcgcggg
      901 acgacatgcg cgggcaggcg tcttcagagc agcccggtgg ccgtgcccag ggaggcgacg
      961 gcgggcagat ccacgcgcgg cgccggcgtc cagccgcgct tgcggtgctc gacgaccacg
     1021 ttggtgtaga tgccgccgcg cacgaaccac ttggccgtga tgcgcacgaa gcgcgggtcg
     1081 gcggccgcga cgatgtcctc gaggatggtg ttggtgaccg cctcgtggaa cgagccctcg
     1141 ttgcggtagc tccacatgta catcttcagg ctcttcaact cgatgcacag ctcgtcgcag
     1201 atcatgtcga tcgtgaagtg cgcgaagtcg ggctggccgg tcagcgggca gtggcaggtg
     1261 aactcgggga tctggaactg gatgacgtag tcgcggcccg acgcggggtt ggcgaagacg
     1321 tgcagctcct tgctcggcgc cgtcggcggg ttgggcggca tctcgcgctc gccgacctgg
     1381 atgcgcccgg ccttggcctt cgcgggcttg gcggcgggtg ccttggacgc ggacttcgcg
     1441 gacggagcgg gaacgggttt cttggccatg tcgtgcctcg gggcggagcg cgcggccgcg
     1501 cgcttgcgcg atcctggttg ggggtgcggg gaagggcggg cgcgggccgc gtcgcgcgtg
     1561 cgggtcgcgg ggcgcgcgtc gcacggggcg ggagtcgcgc cttggggagg cggccagcgg
     1621 agggcgcgat ggtatcatcc cccgcctttt ccggcctcca agagagggct caccacgcgc
     1681 ctcgccgcgg ccggattccc ctcgcgaatc aacggcttgg gcgcggacga cgcaggcggt
     1741 cccggcgacc gcacgcaccg cgcctccctc gtccatgcgg ctcaactcga tcaagctctc
     1801 cggcttcaag tccttcgccg acccgaccaa cttccagctc cccggccagc gcgtgggcgt
     1861 ggtcgggccg aacggctgcg gcaagtccaa catcatggac gcggtgcgct gggtgctggg
     1921 cgagagcaag gcgagcgagc tgcgcggcga gtcgatgcag gacgtgatct tcaacggctc
     1981 cggctcgcgc aagcccgcgg gccgggcgag cgtggagctc gtcttcaaca acgaggccgc
     2041 gcgcgccggc gggccgtgga accagttctc ggagatcgcc gtcaagcgcg tgctcacgcg
     2101 cgagggcgcg tcgagctact acatcaacaa ccagccggtg cgccggcgcg acgtccagga
     2161 cgtcttcctc ggcactggcc tcgggccgcg cgcctacgcg atcatcggcc agggcacgat
     2221 ctcgcgcatc atcgagtcgc ggcccgagga gctgcgcctg ttcctcgagg aggccgcggg
     2281 cgtctcgaag tacaaggagc gccgccgcga gaccgagaac cgcctcaagg acacgcgcga
     2341 caacctgctg cgcgtcgagg acatcctgcg cgagctgaac gcgaacctgg agaagctgga
     2401 gcgccaggcc gaggtcgcga cgaagtaccg cacgctgcag gagcagggca cgctgaagct
     2461 gcaccagctc tggttcctga agcaccgcga cgccgccgcc gagggcgagc gcgtcaagct
     2521 cgcgggcctg gaggcgacca acgcgctcga ggcgcggctc gcggagctgc gtcacgtcga
     2581 ggcccagctc gaagaagtgc gccagcagca ctacggcgca tccgaccggc tgcacggggc
     2641 gcagggcgag ctcgcgcagg cgcagctgga ggtcagccgg ctggaggagc gcatccgcta
     2701 cgtcgtcgag ggccgccagc gcgccgagtc gcgcctggcc gacctgaagt cgcagaacgc
     2761 gcaatggacg gatcgccagg cccaggccga ggccgagctc gagcgcaatg ccgagcagat
     2821 cgccggtgcc ggcgaacagg ccgagctgct ggccgcgcag gcggaggagg aatccctgcg
     2881 cctgcccgac ttcgaggagg cggtgcgcgt cgcgcagcag cgcacgacgg gacagcgcgg
     2941 cgaggtcgtg caggtgcagc agcagatcca ggtgctggcc gccgagagcc gcagcatcga
     3001 ggaacagcag cgtgcgctgc gcggccggcg cgagaagctg gcgggcgagc gctcgcagct
     3061 cgccacgccc gacctgcagc gcctcgagga gctcaaggcc cgcacggccg aggccgacga
     3121 gctcaaggac gagaccgagg cccggctggc cgaggtcgcc gagcaggtgc ccgcgctcga
     3181 cgagcagcgc cgcgcgcagc aggacgtcct gaaccgcgac caggcgcgcc aggcggacct
     3241 gagcgcgcgc ctcgaggcgc tgcgcgcgct gcaggacaag gtgcagaccg agggaaagct
     3301 gaagccgtgg ctggccaggc acggcctgga cgggcttcag ggcctgtgga cgcgcgtgca
     3361 tatcgagccg ggctgggaga cggcgctgga gtcggccctg cgcgagcgcc tgaacgcgct
     3421 ggccgtcggc cggctggaga tggtgcgttc gttcgcgacc gacgcgccgc cggccaagct
     3481 cgcgttctac tcgccgccgg gtgcggccat cgccaacacg catcgcacgc tgccgcggct
     3541 gtcggacctg ctgcgcctga acgacaccgg cctcgccgcg ctgctcaacg actggctcga
     3601 gggcgtctac accgcggcct cgatggacga cgcgctcgcc gcgcgcggcc agctgacgca
     3661 cggcgaggtc atcatgaccc gcgacggcca tgcggtctcg cagttcgccg tcgccttcta
     3721 tgcgccggac tccgagcagg ccggcatgct cgcccgtgcc caggagatcg agcacctgga
     3781 gcgcgagtcg cgcgcgcagg cgctgatcac cgacgaggcg cgcgccgcgc tcgtgcgcct
     3841 ggaggcgcaa tacaccgacg ccgcccagcg cctgcagtcg gtgcggcgcg aggcctcgga
     3901 cgcgcagacg cgcgcgcacg agctgcgcgt cgagctgctg cgcctgtcct cgcaggccga
     3961 ggccgcggcg acgcggcgca cgcagctgga ggacgagctc gccgagatcg acgcccagat
     4021 ggagggcctg gccgagcgcc gggcctccgg cgaggcgcgc ttcgaggagc tggacatgca
     4081 gctggccgtg acccaggagc gccaggccga gctcgaggaa ggcgtcatcc aggccgagcg
     4141 caagctcgcc gacgcgcgcg agcagctgcg cgcgctggag cgccaggcgc aggaggcgca
     4201 gttccagacg cgcgcgctgg ccgcgcgcca gggcgagctg cagcgccaga tcgagacggc
     4261 gcagcagcag gtcgcgcaga acgtgcaggc gggcgagcag ctgacgctgg agctcgacgc
     4321 cttcaacgac caggcggcgc agaccgggct gcaggacgcc ctggagctgc gcgccgcgcg
     4381 cgaggcggcg ctgggcgtgg tgcgcgcgga gtacgacgag ctgtccgcgc agctgcgccg
     4441 cgccgacgag cagcgcatgg agttcgagcg cagcctgcag ccgctgcgcg acgcgatcac
     4501 caagctgcag ctcgagcagc aggcggccga cctcggcggg gcgcagtaca tggagcagct
     4561 cgtggcggcc gaggtcgacc tcgaggcgct cgcacgcaac atcgaggaag gcggcgtgaa
     4621 gctgcacggc ctgcagtcgg agatcgaccg catcggccgc gaggtcaacg cgctcggcgc
     4681 ggtgaacctc gccgcgctcg acgagctggt cgccgcgcgc gagcgcaaga ccttcctcga
     4741 cgcgcagaac accgacctga acgaggccat ccgcacgctg gagggcgcga tccagaagat
     4801 cgacctcgag acgcgcgacc tgctgatgaa gaccttcaac ctggtcaacg agcagttcgg
     4861 ccgcatgttc ccgagcctgt tcggcggcgg ccaggcgaag ctcgtgatga ccggcgacga
     4921 gatcctcgac gccggcgtgc aggtgatggc gcagcccccg ggcaagaaga actcgacgat
     4981 ccacctgctg tccggcggcg agaaggcgct caccgcgatc gcgctggtgt tcgcgatctt
     5041 catgctcaac ccggcgccgt tctgcctgct cgacgaggtc gacgcgccgc tggacgacgc
     5101 caacaccgag cgctacgcca agctggtggc cgagatgtcg cacaagggca cgcagttcct
     5161 gttcatctcg cacaacaaga tcgccatgga gatggccgag cagctgatcg gcgtcacgat
     5221 gcaggagcag ggcgtgtcgc ggatcgtggc cgtggacatg gaggccgccc tgtcgatggc
     5281 cgaagccgcg tgaccatgac gatcaacctg acggtcgcgc tcgctttcgt cgcgctgctc
     5341 gtgctgggcg gcatcgtcgt ccaggcgacg tggaagacgc gccgcatcgc cgcccgccgc
     5401 ggcccggccg tgccgacgcc cggcgagggg ccgcgcgagc cggtgctcgg agagatcgtc
     5461 gtcgacgacc cgctggagct gccgacgccc gcgccggccc gtcccgacag ccgcgccgcg
     5521 ccgctgcgcc gcggcgtcaa gctcgatgcg ctgatcgacg ccatcgcgac gctggccgtc
     5581 gacgcgccgg tcacgggcga ggccgcgctg tcgcacatgc ccgcgaccgc gcgcgccggc
     5641 accaagccgt tcttcatcga gggcctgaac gccgagaccg gcgaatggga ggccgtcgtc
     5701 gcgcacgcgc gctacggcga gttccaggcc ggcgtgcaga tggccaaccg caacggcgcc
     5761 ctgaacgaga tcgagttctc ggagttcgtg cagaagctcg aggcgttcgc gcagggcatc
     5821 ggcgccgcca ccgacctgcc cgacatgctc gagaccgtgg cccgcgcgcg cgagctcgac
     5881 gcgttcgcga gccagcacga cgcgcagctg gtcgcgctgc tgcgcgccaa ttcggtggcg
     5941 tggtcggtcg gctacctgca gcaggtcgcg cagcgccacg gcttcgtgcc cggcgcggtg
     6001 cccgggcggc tcgtgctgcc ggccgccgac gagggcgcgc cgccggtgct ggtgctggct
     6061 ttcgacgccc aggccgcgct ggcggacgat cccggccacg ccgcggtgcg cgaggtctcg
     6121 ctcgggctgg acgtcgcgca gaccgacgag tcggacgagc ccttcgccgc ctggcacggc
     6181 ctggcgcgca agctcgccga ggatctcgat gccgccatcg tcgacgacca gggccagccg
     6241 atcacgctgc acgcgttctc ggccatccat gcggagctcg cgcgcctgta cgaggcgctg
     6301 gccgcgcgcg accttgccgc cggctcggcc ggcgcgcggc ggctcttcag ctagtcgggc
     6361 ccttgtctca acgacctggg cacgcgccgt gccgccgcgg gcgcacaatc gcgccgcgtg
     6421 ccggccgcga cgccacgagc gccgcggca
//
LOCUS       DASVLN010000953         5497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_104697, whole genome shotgun sequence.
ACCESSION   DASVLN010000953 DASVLN010000000
VERSION     DASVLN010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104697"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VF453_22395"
     CDS             <1..719
                     /locus_tag="VF453_22395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690471.1"
                     /translation="AGPRVARIGYLGPSQGGWVAPLAAKAEPVDFVMVAFGLAVSALD
                     EDREAIAINIDAAHGGPQAMAGAMEVADACAALVLHPTLEVFDRFDRVRHHFAREPWL
                     KKVRGDFCGALLGLERETLPQLTQQLSDDTPWLYDPMATIAAVRAPQLWMLAEDDLDA
                     PSAETAHRLAALRLSGRPIATAVFPHTEHGIYEYEAAADGSRASTRQPDGYLRLMVDF
                     ARGVPLKPPYGTAALALPQE"
     gene            complement(738..1655)
                     /locus_tag="VF453_22400"
     CDS             complement(738..1655)
                     /locus_tag="VF453_22400"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690472.1"
                     /translation="MKVFYNPRYNIDLGVLNRLHPFDGRKFGRVAKEIAGVAGLSLVE
                     VPAPLDQAAIERFASPLLQKLLLRKRYVLQALEVPYLPLVPLSFIDRKILEPMRWGAA
                     GTLAAAKAAMQGANCWNLAGGYHHASRTEAEGFCIYNDIGISIQALRESGDLHDLDEV
                     LILDIDAHHGNGNARVFMDDRRIRILDIYDDDIYPRSPYTKERIDINVPLRMGTNGSQ
                     YLSRLDGGLSELPRGARLAFVVAGTDVLASDPLGGLRLTVDDCVARDRLVLDRLAALG
                     VPAVFTGGGGYGPESATAMTRSLLAHAGR"
     gene            complement(1652..2104)
                     /locus_tag="VF453_22405"
     CDS             complement(1652..2104)
                     /locus_tag="VF453_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690473.1"
                     /translation="MQVHIWLPDGEHVGHASFSVGEDYISFWPQDGAGKKDLKIKRSH
                     PGTLVQSLHEDIRNEGHRKPVTVELNALDEDAVLEYVADLRANTPRYQLARNNCSHIV
                     AAALEAGAGAKASFRPHAGHYGTLGRTLGAGIWTPDQVLKFGQELASK"
     gene            complement(2290..2784)
                     /gene="ssb"
                     /locus_tag="VF453_22410"
     CDS             complement(2290..2784)
                     /gene="ssb"
                     /locus_tag="VF453_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7690474.1"
                     /translation="MASVNKVILIGNCGRDPEVRYTPSGTAICNVSVATSSRRKDKTS
                     GESIEDTQWHRVTFYDRLAEIAGEYLKKGKPVYIEGRLKYGKYTDKDGIERNTVDIVA
                     EQMQMLGGREGGGMGGGGMGDEGGGYSPPPARPAARPAPAPAPRPAPKSSTGFDDMDD
                     DIPF"
     gene            complement(2979..3476)
                     /locus_tag="VF453_22415"
     CDS             complement(2979..3476)
                     /locus_tag="VF453_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7690475.1"
                     /translation="MTPAADDLRRKLIRNFDEAELRHEVREPLYDSRGARLAPGTAAR
                     KLGASIDVLAPGKRGCPYHLHHAQEEMFIVLEGRGTLRVAGEMLDIKAGDVIFIPPGR
                     EYPHQIVNTSDAPLKYLSVSTKDSPELCEYPDSGKYQVMTAGPEAFRRVGLVEDDLDY
                     WHREP"
     gene            3825..>5497
                     /locus_tag="VF453_22420"
     CDS             3825..>5497
                     /locus_tag="VF453_22420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX7690476.1"
                     /translation="MPDLHGILQQVFGYSAFRGEQRQIIETVTGGGDALVLMPTGGGK
                     SLCYQVPAIARHRMGHGVTLVVSPLIALMHDQVGALEELGVHAAYLNSSLSMEDAQRV
                     EREMMSGRLVLLYAAPERVNNPRFMAMLSSLNERGLLSLFAIDEAHCVSQWGHDFRED
                     YLQLAQLHENFPDVPRVALTATADDHTRADMRERLALQDAREFISSFDRPNIRYTIVE
                     KDEARKQLLRFLSDEHEGDAGIVYCQSRKKVEETAAWLTAEGIPALPYHAGLDADVRR
                     RHQDRFLREEGLVMCATIAFGMGIDKPDVRFVAHLDLPKNIEGYYQETGRAGRDGEPA
                     DAWMTYGLADVVNQRRMIDESPAGEDFKKKQREKLDALLTLAEAHDCRRARLLAYFGE
                     HYRGPDAQPGSPGRGEPPREAATEASAGVHCGNCDNCLNPPATWDATEAARMVLSTVY
                     RFRQHSGLSFGAGHLIDVLRGKVTDKTTQHGHDGLSTFGIGARLSEPQWRGVVRQLIA
                     LGHLRSEGEYNTLVLAESARAVLKGEVAITMREPREASSRAGGGRTRRAS"
BASE COUNT          830 a         1915 c         1906 g          846 t
ORIGIN      
        1 tcgcgggccc gcgcgtcgcc cgcatcggct acctgggccc gagccagggc ggctgggtcg
       61 cgccgctggc ggccaaggcc gagccggtgg acttcgtgat ggtcgccttc gggctggcag
      121 tctccgccct ggacgaggac cgcgaggcca tcgcgatcaa catcgacgcc gcgcacggcg
      181 ggccgcaggc gatggccggt gcgatggagg tcgcggacgc ctgcgccgcg ctcgtcctgc
      241 atccgacgct ggaggtcttc gaccgcttcg atcgcgtgcg acaccacttc gcgcgggagc
      301 cctggctcaa gaaggtgcgc ggagacttct gcggcgcgct gctgggcctg gagcgcgaga
      361 cgctgccgca gctcacgcag cagttgagcg acgacacgcc ctggctctac gacccgatgg
      421 ccaccatcgc cgcggtgcgc gcgccgcagc tgtggatgct cgccgaggac gacctcgacg
      481 ccccgagcgc cgagaccgcc catcgcctcg cggccctgcg gctgtccggc cgcccgatcg
      541 ccaccgccgt cttcccgcac accgagcacg gcatctacga atacgaggca gccgccgacg
      601 gcagccgcgc gtccacgcgc cagcccgacg gctacctgcg cctgatggtg gacttcgcgc
      661 gcggggtgcc gttgaagccg ccgtacggca ccgcggcgct ggcgctgccg caggagtgag
      721 tgggcggcgc cgcgccgtca gcggcccgcg tgcgccagca ggctgcgcgt catcgccgtc
      781 gcgctctccg ggccgtagcc cccgccgccg gtgaacaccg ccggcacgcc cagcgcggcc
      841 aggcgatcga gcacgagccg gtcgcgcgcg acgcaatcgt cgaccgtcag gcgcaggccg
      901 cccagcgggt cggaggcgag gacgtcggtg ccggcgacga cgaaggcgag gcgggcgccc
      961 cgagggagct cgctcaggcc gccgtcaagt ctggacagat attgcgaacc gttcgtaccc
     1021 atgcgcaagg ggacattgat gtcgatgcgc tccttggtgt acggagatcg gggataaatg
     1081 tcgtcgtcgt agatatcgag tattcggatg cggcgatcgt ccatgaatac acgggcattg
     1141 ccatttccat gatgcgcatc gatgtcgagg atgagcacct cgtccagatc gtgcaaatca
     1201 cccgactcgc gaagcgcctg tatcgagatg ccgatgtcgt tgtagatgca gaagccttcg
     1261 gcctcggtac ggctggcatg atggtaacca cccgccaggt tccagcaatt cgcgccttgc
     1321 atggcggcct tggccgctgc aagcgttccc gcggcacccc aacgcatcgg ctcgagaatc
     1381 ttccggtcga tgaacgacaa aggcaccagc gggagatacg gaacctcgag cgcctgcagg
     1441 acatagcgct tgcgcagcaa cagcttctgc agcagcgggc tcgcgaaccg ctcgatcgca
     1501 gcttggtcga gcggcgcggg cacttcgacc agactcaacc cggcgacacc tgcaatctcc
     1561 tttgccacac ggccgaactt gcggccgtcg aacgggtgca gcctgttcag gacacccagg
     1621 tcgatgttgt agcgggggtt gtagaagacc ttcacttgct ggccagctcc tgcccgaatt
     1681 tcagtacctg atcgggcgtc cagatacccg cgccgagggt gcgtcccaat gtaccgtagt
     1741 gccccgcgtg cggacgaaag gaggcttttg ccccggcgcc agcctcgagc gcagcggcga
     1801 ctatatggga gcaattgttt cgcgccagct gataccgagg agtgttggcg cggagatctg
     1861 ccacatactc cagcaccgca tcctcgtcga gcgcgttcag ttcgacggtg acaggcttgc
     1921 ggtggccttc attccggata tcttcgtgaa gactctgaac caaggtgccg gggtgactgc
     1981 gcttgatctt cagatctttc tttcccgccc cgtcctgcgg ccagaagctg atgtaatcct
     2041 cgccgaccga aaacgacgcg tgcccgacat gctccccgtc gggaagccag atatgtacct
     2101 gcattccatc ccagctgaat tgttcgaatc gctgacctgt cgctcaaccg cgtggcggca
     2161 attcgatgag gtcaggccgc gtgttgtagc agaacgcgcg acgacgagct tcccgcatcc
     2221 gagggatgga aaaaggccgg cgccttgcgg taaccggccc tgtttacgcg atggacgccc
     2281 gcatgggctt cagaacggaa tgtcgtcatc catgtcgtcg aaccccgtcg acgacttggg
     2341 cgccgggcgc ggcgcgggag ccggtgcggg gcgggcggcg gggcgcgccg gcggggggct
     2401 gtagccgccg ccttcgtcgc ccatgccgcc gccgcccatg ccgccgccct cgcggccgcc
     2461 gagcatctgc atctgctcgg cgacgatgtc gacggtgttg cgctcgatgc cgtccttgtc
     2521 ggtgtacttg ccgtacttca ggcggccctc gatgtagacc ggcttgccct tcttcaggta
     2581 ctcgccggcg atctcggcca ggcggtcgta gaaggtgacg cggtgccact gcgtgtcttc
     2641 gatgctctca ccgctggtct tgtccttgcg gcggctggac gtcgcgaccg agacgttgca
     2701 gatcgcggtg ccgctggggg tgtagcggac ttccgggtcg cgcccgcagt tgccgatgag
     2761 gatgactttg ttgactgacg ccatggcgtg ctccaggaaa ggtggggacg gctccccgtg
     2821 gccggccgcc gtgcaaggcg gcttgggatg gtgtggcgtc ccgcggccgc gctcgcgtcc
     2881 gggggacagg agcgattatg ccggcgcccc gccggcggcc ggcgcgcgga ttcccgcgaa
     2941 aacgcggcgt ttcgcgcggg atgcggcggc cgcgtcggtc acggttcgcg gtgccagtag
     3001 tcgaggtcgt cctcgaccag cccgacgcgg cggaacgcct ccggcccggc cgtcatcacc
     3061 tggtacttgc cggagtcggg atactcgcag agctcgggcg aatccttggt gctgaccgac
     3121 aggtacttca gcggcgcgtc cgaggtgttg acgatctggt gcgggtactc gcgccccggc
     3181 gggatgaaga tcacgtcgcc cgccttgatg tccagcatct cgccggccac gcgcagcgtg
     3241 ccgcgtccct cgagcacgat gaacatctcc tcctgcgcat ggtgcaggtg gtacgggcaa
     3301 ccgcgcttgc ccggcgcgag gacgtcgatg gacgcgccca gcttgcgcgc cgcggtgccc
     3361 ggcgccaggc gcgcgccgcg gctgtcgtac aggggctcgc gcacctcgtg gcgaagctcg
     3421 gcctcgtcga agttgcggat gagcttgcgg cgcaggtcgt cggcggcagg ggtcatgcgg
     3481 gtctccggtc ggttcggttt cgggaagggc gacggccatt ctgcgacagc gacgcttgcc
     3541 ctcgatggcc gtgcggcccg ttccgcgaac gcgccgtcga cgcggcggcc cgcggtcgag
     3601 ccggcgcggc cccgggccgc acaaatcgcc cgaaaccgag ggatttgacg ccggggcggc
     3661 ccgtcgtgcc ctgtaccatg gagccttgtt tcacccgggg ccggccgacg gcggcgcccc
     3721 gcgccgcgac ggcccttcct cccgtgtccg aattcgacta cgactacgac ttcgactccc
     3781 tgcccacgac gcgcccggcg cccgtggcgc gcgagccggg gcccatgccc gacctgcacg
     3841 ggatcctgca gcaggtcttc ggctattcgg ccttccgcgg cgagcagcgg cagatcatcg
     3901 agacggtcac cggcggcggc gacgcgctgg tgctgatgcc caccggtggc ggcaagagcc
     3961 tgtgctacca ggtgccggcg atcgcccgcc atcgcatggg ccacggcgtc acgctggtcg
     4021 tgagcccgct gatcgcgctg atgcacgacc aggtgggcgc gctggaggag ctgggcgtgc
     4081 atgccgccta cctcaactcc tcgctgtcga tggaggacgc gcagcgcgtc gagcgcgaga
     4141 tgatgagcgg ccgcctggtg ctgctgtacg ccgcgcccga gcgcgtcaac aacccgcgct
     4201 tcatggcgat gctgtcgtcg ctgaacgagc gcggcctgct gagcctgttc gcgatcgacg
     4261 aggcgcactg cgtcagccag tggggccacg acttccgcga ggactacctg cagctcgcgc
     4321 agctgcacga gaactttccc gacgtcccgc gcgtggcgct caccgccacc gccgacgacc
     4381 acacccgcgc cgacatgcgc gagcggctcg cgctgcagga tgcgcgcgag ttcatctcga
     4441 gcttcgaccg cccgaacatc cgctacacca tcgtcgagaa ggacgaggcg cgcaagcagc
     4501 tgctgcgctt cctgtccgac gagcatgagg gcgacgccgg catcgtctac tgccagagcc
     4561 gcaagaaggt cgaggagacg gctgcctggc tgaccgccga gggcatcccg gcactgccct
     4621 accacgccgg cctcgacgcc gacgtgcgcc gccgccacca ggatcgcttc ctgcgcgagg
     4681 aagggctggt catgtgcgcg acgatcgcct tcggcatggg catcgacaag cccgacgtgc
     4741 gcttcgtcgc ccacctggac ctgcccaaga acatcgaggg ctactaccag gagaccggac
     4801 gcgcgggccg cgacggcgag cccgcggacg cctggatgac ctacggcctg gccgacgtcg
     4861 tcaaccagcg ccgcatgatc gacgagagcc cggccggcga ggacttcaag aagaagcagc
     4921 gcgagaagct cgacgcgctg ctcacgctgg ccgaggcgca cgactgccgg cgcgcgcggc
     4981 tgctcgccta cttcggcgag cactacagag gccccgacgc gcaaccgggt tccccgggcc
     5041 gcggcgagcc ccctcgggag gcggcgaccg aagcgagcgc gggggtccat tgcggcaact
     5101 gcgacaactg cctcaacccg cccgcgacct gggacgcgac cgaggccgcg cgcatggtgc
     5161 tgtccaccgt ctaccgcttc cgccagcact cgggcctgag cttcggggcc ggccacctca
     5221 tcgacgtgct gcgcggcaag gtcaccgaca agacgacgca gcacggccac gacgggctgt
     5281 cgaccttcgg catcggcgcc cggctctcgg agccgcagtg gcgcggcgtc gtgcggcagc
     5341 tgatcgcgct cggccacctg cgctcggagg gcgagtacaa cacgctcgtg cttgccgaga
     5401 gcgcgcgcgc cgtgctcaag ggcgaggtcg cgatcacgat gcgcgagccg cgcgaggcgt
     5461 cgtcccgggc cggcggcggc cggacgcggc gggcgtc
//
LOCUS       DASVLN010000954         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_48111, whole genome shotgun sequence.
ACCESSION   DASVLN010000954 DASVLN010000000
VERSION     DASVLN010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48111"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /gene="glmU"
                     /locus_tag="VF453_22425"
     CDS             <1..1128
                     /gene="glmU"
                     /locus_tag="VF453_22425"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX7690477.1"
                     /translation="PALPDEGTTLVLNGDVPLIRPGTLRALVDACGGRHLALLTVTLA
                     DPFGYGRIVRAPEAGDGDAIGAVQAIVEEKDATPLQRQIGEIYTGIMAAPSGLLKSWL
                     ARLTNDNAQREYYLTDVVALAVADGVEVATAAAADETEVLGVNSPAQLADLERRLQRR
                     RADALLEAGVRLADPARFDLRGTLACGRDVEIDVGCVFEGDVRLGDDVRVGPHCVIRD
                     ADIAAGAVIHAFTHIEGARVGAGALIGPYARLRPGAALAEDVHIGNFVEVKNSTLARG
                     AKANHLAYLGDASVGERVNFGAGAITANYDGANKHRTTIGADVHVGSNCVLIAPVTLG
                     DGGTIGGGSTISKDTAAGALTVARAKQVSIAGWSRPKKAPK"
     gene            complement(1146..>1830)
                     /locus_tag="VF453_22430"
     CDS             complement(1146..>1830)
                     /locus_tag="VF453_22430"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7690478.1"
                     /translation="AAAAAALQATLSDPAMIERAATIATLDWPALKAAVAECRACGLC
                     ESRRQTVFGVGHAKADWMIVGEAPGEQEDLQGEPFVGPAGQLLDQMLRAVGQSRFVDG
                     ERADDDVGDPSRRVFIANTLKCRPPRNRNPEPDEMARCEPFLKRQLALVQPKLILAMG
                     RFAVKQLLRSDEAIGRLRGRVHRYEGVPVIVTYHPAYLLRNLPDKAKAWEDLCLARRV
                     ADEGAAAAG"
BASE COUNT          245 a          687 c          680 g          218 t
ORIGIN      
        1 cccgcgctgc ccgacgaggg cacgacgctc gtcctcaacg gcgacgtccc gctgatccgc
       61 cccgggacgc tgcgcgcgct ggtcgacgcc tgcggcggcc gccacctggc gctgctgacg
      121 gtgacgctgg ccgacccgtt cggctacggc cgcatcgtgc gcgcgcccga ggccggcgac
      181 ggcgacgcca tcggggcggt gcaggcgatc gtcgaggaga aggacgcgac gccgctgcag
      241 cgccagatcg gcgagatcta caccggcatc atggccgcgc cgagcgggct gctgaaatcc
      301 tggctcgcgc gcctgaccaa cgacaacgcg cagcgcgagt actacctgac cgacgtcgtc
      361 gccctcgcgg tggccgacgg cgtcgaggtg gcgacggccg ccgccgccga cgagaccgag
      421 gtgctgggcg tcaacagccc ggcgcagctt gccgacctcg agcgccgcct gcagcgccgg
      481 cgcgccgacg cgctgctgga ggccggcgtg cgcctggccg acccggcgcg cttcgacctg
      541 cgcggcacgc tcgcctgcgg ccgcgacgtg gagatcgacg tcggctgcgt cttcgagggc
      601 gacgtccgcc tgggcgacga cgtgcgcgtc ggcccgcact gcgtgatccg cgacgccgac
      661 atcgccgccg gcgccgtcat ccacgcgttc acgcacatcg agggcgcgcg cgtcggcgcg
      721 ggggcgctga tcgggccgta cgcgcgcctg cgcccgggcg ccgcgctggc cgaggacgtg
      781 cacatcggca acttcgtcga ggtcaagaac tcaacgctcg cccgcggcgc gaaggccaac
      841 cacctcgcct acctcggcga cgcgagcgtg ggcgaacggg tgaacttcgg cgcgggcgcg
      901 atcaccgcca actacgacgg cgccaacaag caccgcacga cgatcggcgc ggacgtgcac
      961 gtcggctcca actgcgtgct gatcgccccg gtgacgctgg gcgacggcgg caccatcggc
     1021 ggcggctcga cgatcagcaa ggacaccgcc gccggcgcgc tgaccgtggc gcgggcgaag
     1081 caggtctcga tcgcgggctg gtcgcggccg aagaaggcgc cgaagtagtc ggtcgggcgc
     1141 cggcgtcagc cggccgcggc cgcgccctcg tccgcgacgc gccgggcgag gcacaggtcc
     1201 tcccacgcct tcgccttgtc gggcaggttg cgcagcaggt aggccgggtg gtaggtcacg
     1261 atgacgggga cgccctcgta gcggtgcacg cgaccgcgca gccggccgat ggcctcgtcg
     1321 gagcgcagca gctgcttcac ggcgaagcgg cccatcgcga ggatgagctt cggctgcacg
     1381 agcgcgagct ggcgcttgag gaagggctcg cagcgcgcca tctcgtccgg ctcgggattg
     1441 cggttgcgcg gcgggcggca cttgagcgtg ttggcgatga agacgcgccg cgacgggtcg
     1501 ccgacgtcgt cgtccgcccg ctcgccgtcg acgaagcgcg actggccgac cgcgcgcagc
     1561 atctggtcga gcagctggcc cgcggggccg acgaagggct cgccctggag gtcctcctgc
     1621 tcgcccggcg cctcgccgac gatcatccag tcggccttcg catggccgac gccgaagacg
     1681 gtctgccggc ggctctcgca cagcccgcag gcccggcact cggcgaccgc ggccttcagc
     1741 gcaggccagt ccagcgtcgc gatcgtggcc gcgcgctcga tcatcgccgg atccgacagc
     1801 gtcgcctgca gcgccgccgc ggccgccgcc
//
LOCUS       DASVLN010000955         5969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_48188, whole genome shotgun sequence.
ACCESSION   DASVLN010000955 DASVLN010000000
VERSION     DASVLN010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48188"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1022
                     /locus_tag="VF453_22435"
                     /pseudo
     CDS             102..1022
                     /locus_tag="VF453_22435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
     gene            1398..2999
                     /locus_tag="VF453_22440"
     CDS             1398..2999
                     /locus_tag="VF453_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX7690479.1"
                     /translation="MNSSFDEMYAADGAVRAHYQGYARWLEQQPADLMRDRRNEAEVI
                     FRRVGITFAVYGDKDAGEGTERLIPFDQIPRVIPSSEWREMDKGLRQRVTALNRFVHD
                     VYHGQEILRAGLVPASQVLANAQYRKEMAGVDVAGDVYSHIAGIDIVRAGNADGSGSY
                     YVLEDNLRVPSGVSYMLENRKMMMRLFPELFSLHSIAPVAHYPDLLLETLRSVAPGGA
                     NDPTVVVLTPGMYNSAYFEHAFLAQQMGIELVEGQDLFVKDDYVFMRTTQGPKRVDVI
                     YRRIDDDFLDPEAFRRDSALGCAGLLRAYRAGNVTLSNAIGTGVADDKSIYPYVPKMI
                     EFYLGEKPILNNVPTYQCREADDLAYVLDHLGELVVKEVHGAGGYGMLVGPAATKAEI
                     EAFRAQLKARPDNYIAQPTLSLSTCPTYVESGIAPRHIDLRPFVLSGKAVQVVPGGLT
                     RVALKEGSLVVNSSQGGGTKDTWVLESSSPSGNGRGPGAGGGAGESPSGSQEGQRQGG
                     AQGAQARSGSPSQSQSQSSSQTPSA"
     gene            3013..3966
                     /locus_tag="VF453_22445"
     CDS             3013..3966
                     /locus_tag="VF453_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX7690480.1"
                     /translation="MLSRTADHLFWMARYMERAENTARMLDVNYQSSLLPQSEAVAEL
                     GWRGLLSISELTGDFGQRYGEVTPSGVMEFMVSDGQNLSSILSCLRAARENARAVRGT
                     LTTEVWETTNQTWLEFNKMLATGALRRDPASVFEWVKFRSHLSRGVAVGTMLQDEAFH
                     FVRIGSFLERADNTARLLDVKFHAVESDFYGRADERDNEYEFYHWSAILRSVSGFEVY
                     RKVYRNVIQPEKVAELLILRQDMPRSLAHCVGRLQSNLHHVANIQSAETLRRAGRLEA
                     DLRFGRIDEILSTGLHAYLTQFLANVNDLGMGISRDFLVPQ"
     gene            4220..>5969
                     /locus_tag="VF453_22450"
     CDS             4220..>5969
                     /locus_tag="VF453_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX7690481.1"
                     /translation="MAIHVALNHVTSYKYDRLVALGPQVVRLRPAPHCRTPILSYSLK
                     IEPSGHFVNWQQDPFANYLARLVVPEKVREFKVTVDLVAEMAVYNPFDFFLEESAGKF
                     PFAYDEALAAELAPYLVADEAGPLLAAYLRTIPREPRATVDFLVDLNQRLQSDVRYLI
                     RMEPGVQTPEETLAKGCGSCRDSGWLMVQLLRHLGLAARFVSGYLIQLAPDQEALDGP
                     SGTDIDFTDLHAWCEVYLPGAGWIGFDPTSGLLAGEGHIPLACTPQPSSAAPISGGVD
                     ESEVDFSHHMAVSRIWESPRVTLPYTEEQWRAVLALGDAVDARLVAGDVRLTMGGEPT
                     FVSVDDRDGAEWNTDALGPTKRMRAQELVLRLRDHYGRGGFLHFGQGKWYPGEQLPRW
                     ALSICWRADGVPCWNDPRLFADEREPQHYTADDARRFATTLARRLGIADTTVQPGFED
                     TFYYLWRERRLPVNVDPFDARLADELERNRLRRVFAQGLDAPVGYVLPLRREHGPGLA
                     GGHWVTGPWHVRDERLYLIPGDSPMGYRLPLDSLPWVRDTEFPWLAERDPFDPRGPLP
                     GTFGDGTAPGRPGSGAG"
BASE COUNT          828 a         2158 c         2087 g          896 t
ORIGIN      
        1 ccgcgctgcc ggccctgttc gcgcgcggcc tggcccgcga gctgcgcggc aacgcgcggg
       61 cgcaggtggt cgcgagcatc ggcctcgcgc cgctcacctt ggtggccttc cagcaggtgt
      121 cgctggtggg tctgctcgcc aacctggtgg ccgagccgtg gatcacgctc gccgtcacgc
      181 ccctgacgct gctgggcatc gtcgtcccgg gcgcctggac gctcgcggcg ctcgcgctgc
      241 ggccgctgct cgcctggctc ggttggctcg cgcggtggcc ctgggcgagc gtgcatgtcg
      301 ccacggcacc gggatgggcg ctcgtcgccg ggctcgtcgg cggcacgctg ttgatgacgc
      361 cgcttccatg gcgcctgcgt gcgctgggtc tcgcgctgct gccgccgttg gtggcgccgt
      421 tcgtcgcccg gccgcccgca gggcgcttcg agctggtcgc ggccgatgtg ggccagggct
      481 cggcggtgct ggtgcggaca cggacgcacc tcctgctgtt cgacaccggc ccgcgcttcg
      541 gagaggacaa cgacgccggc cgccggatgc tgctgccgct gctgcagtcg cgcggcgagt
      601 cgcgcatcga cctcctggtg ctgagccatg ccgacgccga tcacgtcggc ggcgcccagg
      661 ccatcatcga ccgcttcccc gtcgcagcga tgcgcagctc gctggaggcc ggccacccgc
      721 tgcgcgcgaa cccgatcccg caccggcctt gcgaagccgg cgacgcgtgg gactgggatg
      781 gcgtgcgctt ccgcttcctg catccgccgc tcgcggcgct gcaccccggt gcgaagacga
      841 atgccatcag ctgcgtgctg cgggtcgtgg atgcgctcgg gcgcagcgcg ctgctgaccg
      901 gcgacgtcga ggcgccgcag gaagcctggc tcgtcgcgcg ctccgccgcc gcgccacagg
      961 gccggctcga ggatgcggct ctgcgcagcg acatcctcgt cgtcccccac cacggaagcc
     1021 atacgtcctc gaccgcggcc ttcctcgacg ccgtgcgacc ccgggtggcg gtgatccagg
     1081 tcggctaccg cagtcgatac gggcatccgg cgccggcggt cgtggcgcgc tacggggcgc
     1141 gcggcatcgc cgtggtgcgc accgaccact gcggtgcatg gacctggcgc gacggcgaag
     1201 gtgtttgcac gcgcacggtg cgtcgtcgat actggggctg gcagggaacg tcgggatccg
     1261 agccggcggc gcccgggacg gcgggtggca cagcctcccc gaccggggcg cgagctggat
     1321 cggatgttgc tatccaactc ggcgacacgt cgcgcggcgc gcgtgtcgcc agcctgagag
     1381 agggccagtc gctcgccatg aattcgtcct tcgatgagat gtacgccgcc gacggcgcgg
     1441 tgcgtgcgca ttaccagggc tatgcccgct ggctggagca gcagcccgcc gacctgatgc
     1501 gcgaccggcg caacgaggcc gaggtgatct tccggcgcgt gggcatcacc ttcgcggtct
     1561 acggcgacaa ggacgcgggc gaaggcaccg agcgcctcat cccgttcgac cagatcccgc
     1621 gcgtgatccc gtcctccgag tggcgtgaga tggacaaggg cctgcgccag cgcgtgacgg
     1681 cgctcaaccg cttcgtccac gacgtctacc acgggcagga gatcctccgg gccggcctgg
     1741 tccccgcgag ccaggtgctc gccaacgcgc agtaccgcaa ggagatggcg ggcgtggacg
     1801 tggccggcga cgtctactcg cacatcgccg gcatcgacat cgtccgcgcc ggcaacgccg
     1861 acggctccgg cagctactac gtgctggagg acaacctgcg ggtgcccagc ggcgtgtcct
     1921 acatgctcga gaaccgcaag atgatgatgc ggctgttccc ggagctcttc tcgctgcact
     1981 cgatcgcgcc ggtcgcgcac taccccgacc tcctgctcga gacgctgcgc agcgtcgccc
     2041 ccgggggcgc caacgatccg acggtcgtcg tgctcacgcc gggcatgtac aactcggcct
     2101 acttcgaaca cgcgttcctc gcgcagcaga tgggcatcga gctggtcgag ggccaggacc
     2161 tgttcgtcaa ggacgactac gtcttcatgc gaacgacgca gggccccaag cgcgtcgacg
     2221 tgatctaccg ccgcatcgac gacgacttcc tcgaccccga ggcattccgc cgggattccg
     2281 cgctcggctg cgcgggcctg ctgcgggcct accgcgccgg caacgtgacg ctgtccaacg
     2341 cgatcggcac cggcgtggcg gacgacaagt cgatctaccc ctacgtgccg aagatgatcg
     2401 agttctacct cggagagaag ccgatcctga acaacgtccc gacctaccag tgccgggagg
     2461 cggacgacct tgcctacgtc ctcgaccacc tgggcgagct ggtcgtcaag gaggtgcacg
     2521 gggcgggggg ctacgggatg ctggtcggcc cggcggcgac caaggcggag atcgaggcct
     2581 tccgcgccca gctcaaggcg cggccggaca actacatcgc ccagccgacg ctgagcctgt
     2641 cgacctgccc gacctacgtc gagtcgggga tcgcgccgcg ccacatcgac ctgcggccgt
     2701 tcgtgctttc gggcaaggcg gtgcaggtcg tgccgggcgg cctgacccgg gtggcgctca
     2761 aggagggctc gctggtggtg aacagcagcc agggcggcgg cacgaaggac acctgggtgc
     2821 tggagtcttc ctcgccgagc gggaacgggc gcgggccggg cgcgggtggc ggcgcaggcg
     2881 agtcgccgtc cgggtcgcag gagggacagc ggcaaggcgg cgcgcagggc gcgcaggcgc
     2941 gatccggctc gccctctcag tcgcagtccc agtcgtcgtc gcaaacaccc tcggcctgag
     3001 gagcccgcca agatgctttc ccgcaccgcc gaccacctgt tctggatggc ccgctacatg
     3061 gagcgcgccg agaacaccgc gcgcatgctc gacgtcaatt accagagctc gctgctgccg
     3121 cagtccgaag ccgtcgcgga gctgggctgg cgcgggctgc tgtcgatcag cgagctcacc
     3181 ggcgactttg gccagcgcta tggcgaggtc acgccgtcgg gggtgatgga gttcatggtc
     3241 agcgacggcc agaacctgtc gtccatcctc agctgcctgc gagcggctcg cgagaacgcc
     3301 cgcgcggtgc ggggcacgct cacgacggaa gtctgggaga cgaccaacca gacctggctc
     3361 gagttcaaca agatgctcgc cacgggagcg ctgcggcgcg atccggcgag cgtgttcgaa
     3421 tgggtcaagt tccgctccca cctgtcgcgc ggcgtcgcgg tgggcacgat gctgcaggac
     3481 gaggccttcc acttcgtgcg gatcggctcc ttccttgaac gcgccgacaa caccgcgcgg
     3541 ctgctggacg tgaagttcca cgccgtcgag agcgacttct acggccgtgc cgacgagcgc
     3601 gacaacgagt acgagttcta ccactggtcg gcgatcctgc gctcggtctc cggcttcgag
     3661 gtctatcgca aggtctaccg caacgtcatc cagccggaga aggtcgccga gctgctgatc
     3721 ctgcgccagg acatgccgcg ctcgctcgcg cattgcgtcg gccggctgca gagcaacctt
     3781 caccatgtcg ccaacatcca gtcggccgag acgctgcgcc gtgccggccg cctggaggcc
     3841 gacctgcgct tcggtcgcat cgacgagatc ctgtccaccg gtttgcacgc ctacctcacc
     3901 cagttcctcg ccaacgtgaa cgacctgggc atgggcatca gccgcgactt cctcgtgccg
     3961 caatgagccg tcccgtccgc ctccgcgccg gccgcgtccg agggccgcgt gcgcgagctc
     4021 gcggccgtgc gccgacctgc cgtcctcggc accgacactg acaccgcgga tgtggcgccc
     4081 gccggcgcgc gcatcgccca gagagaaatg ccgccggtgc tcgagcgacg cgaaggcgcg
     4141 tcgcgcctcc ggcccgtcac cgccgctcag ccgcgccgcc cgaggagggg gcgcgggccc
     4201 gtcatcgcag gagtcgtcct tggccatcca tgtcgcgctg aaccatgtca cgtcgtacaa
     4261 gtacgaccgc ctcgtcgccc tcggcccgca ggtggtgcgg ctgcggccgg ccccgcattg
     4321 ccgcacgccc atcctgtcgt actcgttgaa gatcgagccg tccgggcact tcgtcaactg
     4381 gcagcaggat ccgttcgcga actacctcgc gcgcctcgtc gtgccggaga aggtgcgcga
     4441 attcaaggtc accgtggatc tcgtcgccga gatggcggtc tacaacccgt tcgacttctt
     4501 tctcgaggag agcgccggca agttcccgtt cgcctacgac gaggcgctcg ccgccgagct
     4561 cgcgccttac ctggtcgcgg acgaggcggg tccgctcctc gcggcatacc tgcgcacgat
     4621 cccgcgcgag ccgcgcgcca ccgtcgactt cctcgtcgac ctcaaccagc gtttgcagtc
     4681 cgacgtccgc tacctgatcc gcatggagcc cggcgtgcag acgccggagg agacgctggc
     4741 caagggctgc ggctcctgcc gcgactcggg ctggctgatg gtgcagctgc tgcgccacct
     4801 cgggctggcg gcgcgcttcg tctcgggcta cctgatccag ctcgcgcccg accaggaagc
     4861 gctcgacggg ccgagcggca cggacatcga cttcaccgac ctgcacgcat ggtgcgaggt
     4921 ctacctgccc ggcgcgggct ggatcggctt cgaccccacc tccggcctgc tcgccggcga
     4981 gggccacatc ccgctcgcgt gcacaccgca accttcgagt gcggcgccga tctcgggcgg
     5041 cgtcgacgag agcgaggtgg acttcagcca tcacatggcg gtctcgcgga tctgggagtc
     5101 gccgcgcgtc acgttgccct acaccgagga gcagtggcgg gcggtgctcg cgctgggaga
     5161 cgccgtggac gcgcgcctcg tcgccggcga cgtgcggctg acgatgggcg gcgagccgac
     5221 cttcgtgagc gtcgacgacc gtgacggcgc cgagtggaac accgacgcgc tggggccgac
     5281 caagcgcatg cgggcgcagg agctggtgct gcgcctgcgc gaccactatg gccgcggagg
     5341 cttcctgcac ttcggccagg gcaagtggta ccccggcgag caattgccgc gctgggcgct
     5401 ctcgatctgc tggcgcgccg acggcgtgcc gtgctggaac gacccgcgcc tgttcgccga
     5461 cgagcgcgag ccgcagcact acaccgccga cgacgcgcgg cgcttcgcca cgacgctcgc
     5521 gcgccgactg ggaatcgcgg acacgaccgt gcagcccggc ttcgaagaca ccttctatta
     5581 cctctggcgc gagcgccggc tgcccgtcaa cgtcgatccc ttcgacgctc gcctggccga
     5641 cgagctcgag cgcaatcgcc tgcgccgggt gttcgcccag ggcctggacg caccggtcgg
     5701 ctacgtgctg ccgctgcggc gcgagcacgg gccggggctg gcaggcggcc actgggtcac
     5761 ggggccgtgg cacgtgcgcg acgagcgcct ctacctgatc ccgggcgatt cgccgatggg
     5821 ctaccggctg cccctggact cgctgccgtg ggtgcgcgac accgagttcc cctggctcgc
     5881 cgagcgcgat ccgttcgacc cgcgcggccc gctgccgggc acgttcggcg atggcactgc
     5941 tccggggcgg cccggatccg gcgcgggct
//
LOCUS       DASVLN010000956         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_426946, whole genome shotgun sequence.
ACCESSION   DASVLN010000956 DASVLN010000000
VERSION     DASVLN010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426946"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..2652)
                     /gene="mgtA"
                     /locus_tag="VF453_22455"
     CDS             complement(10..2652)
                     /gene="mgtA"
                     /locus_tag="VF453_22455"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEX7690482.1"
                     /translation="MHEPAPDVDAFWTRPLQRQWDELRSSERGLGEEAARSLRAVHGD
                     NVLRVERRRALAVEYLSHFRNPLVLLLLAASAASALLGDVTSFAVIGLIVLGSVTLDV
                     VQEHRAATAATRLRDSVAVRATVLRDGAPTRLPAAQLVPGDVVRLAAGDLVPADGRVV
                     EARDFFVRQSALTGESFPVEKHAGDVEAGATSLVDATNAAFMGSSVLSGSATLLVCRT
                     GPSTFLGGIADTLREPAGPTAFERGTRGFGLLVLRMTTALVLFIAFVHLLANRPVQET
                     FMFAVALAVGLTPELLPMVVSVTLSRGALRMAARKVVVKRLAAIQNMGSMDVLCTDKT
                     GTLTEAAIRLERCVDARGRDSRRVRELAYVNSSLESGLRSPLDDAILACRDVDAAGWS
                     KVDEVPFGFERRRVSVLAERGGERLLVVKGAPEDMLRICDRLEDGASTDGPVATAPMD
                     DGARARCLERFEALSRDGLRVLAVAWRAVPRDHAHAVVDDESQLVLCGFAVFLDPPKA
                     SAAAALERLRRSHVRVKVLTGDNEWVTRHVCAQVGLPVKHLLTGAQIDQLDDGSLAVQ
                     AQRADAFCRVSPPQKSRILRALRRRGHVVGFLGDGVNDAPSLHEADVGISVDSAVDVA
                     RDAADMILLEHDLGVLHEGVVEGRRTFANVMKYIMMGTSSNFGNMFSMAGASLILPFL
                     PMLPVQILLNNLLYDVSEVPIPLDDVDPDYVQRPHDWDPGFIRRFMLTLGPVSSLFDF
                     ATFWLLVSAFHARAELFRTGWFIESIATQVLVIFVIRTRGAPWRSRPASWLVAAAVAV
                     VLLAVALPFSPAAGLLGFVAPPATLLAVIALLTLVYLGCAELAKRWFYRRQGARSRRG
                     APGAMRGTASASRAAARRGHQPTA"
     gene            complement(2690..3073)
                     /locus_tag="VF453_22460"
     CDS             complement(2690..3073)
                     /locus_tag="VF453_22460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690483.1"
                     /translation="MSHHAVIRIDQAHALVLQLDSHPPLARRLEAHRHPTRRHASAAR
                     TGHEFLAEVCAAVDDVDEILVVGGHQALADFEHYVRKHRPETAARIVGNDVVDQLSQS
                     QLAAHARRFFERRAHLADIGALPGG"
BASE COUNT          491 a         1221 c         1261 g          409 t
ORIGIN      
        1 gggcggcgct caggcggtcg gctggtggcc tcgccgtgcc gcagccctgg acgcggacgc
       61 cgtcccgcgc atcgctcccg gggcgccgcg gcgcgacctc gcgccctggc ggcgatagaa
      121 ccagcgcttg gcgagctccg cgcaaccgag gtacacgagc gtcagcagcg cgatcacggc
      181 cagcagcgtc gcgggcggcg cgacgaatcc gagcaggccc gcggccggcg agaacggcag
      241 cgccacggcc agcagcacca ccgcgacggc cgccgccacc agccacgaag cggggcggct
      301 tcgccatggc gcgccgcgcg ttcggatgac gaagatcacg agcacctgcg tggcgatcga
      361 ctcgatgaac catcccgtgc ggaacagctc ggcccgcgcg tggaaggccg agaccagcag
      421 ccagaaggtg gcgaagtcga acagcgagct gaccggcccg agcgtcagca tgaagcggcg
      481 gatgaagccc ggatcccagt cgtggggccg ctgcacgtag tccgggtcga cgtcgtcgag
      541 cggtatcggc acctcggaga cgtcgtagag caggttgttc agcaggatct gcaccggcag
      601 catcggcagg aagggcagga tcagcgaggc gcccgccatg ctgaacatgt tcccgaagtt
      661 cgagctcgtc cccatcatga tgtacttcat cacgttggcg aacgtgcgcc ggccctcgac
      721 cacgccctcg tgcaggacgc cgaggtcgtg ttccagcagg atcatgtcgg ccgcgtcgcg
      781 cgcgacgtcg acggccgaat ccacggagat gccgacgtcg gcttcatgca gggacggcgc
      841 gtcgttcacg ccgtcgccga ggaagcccac gacgtggccg cgccggcgca gcgcacgcag
      901 gatgcggctc ttctgcggcg gcgagacgcg gcagaacgca tcggcgcgct gggcctgcac
      961 cgcgagcgag ccgtcgtcga gctggtcgat ctgcgcgccc gtcagcaggt gcttcaccgg
     1021 cagcccgacc tgcgcgcaga cgtggcgcgt gacccactcg ttgtcgccgg tgagcacctt
     1081 gacccgcacg tggctgcgcc gcagccgctc cagggccgcg gcagcgcttg ccttcggcgg
     1141 atccaggaag acggcgaagc cgcacagcac gagctgcgac tcgtcgtcga cgaccgcgtg
     1201 ggcgtggtcg cgcggcaccg cgcgccaggc gacggcgagc acgcgcaagc cgtcgcggct
     1261 cagcgcctcg aagcgctcga ggcagcgcgc gcgggcgccg tcgtccatcg gcgcggtggc
     1321 gacgggcccg tccgtggacg cgccgtcctc gaggcggtcg cagatgcgca gcatgtcctc
     1381 gggcgcgccc ttgacgacca gcaggcgctc gccgccgcgt tcggccagca ccgacacgcg
     1441 gcggcgctcg aagccgaagg gcacttcgtc gaccttgctc cagccggccg cgtcgacgtc
     1501 gcggcaggcg aggatcgcgt cgtccagcgg gctgcgcagg cccgactcga ggctgctgtt
     1561 cacgtaagcg agctcgcgca cccggcggct gtcgcggccg cgggcgtcga cgcagcgttc
     1621 caggcggatg gcggcttccg tgagcgtgcc cgtcttgtcg gtgcacagca cgtccatgct
     1681 gcccatgttc tggatcgcgg cgagacgctt gacgacgacc ttgcgcgccg ccatgcgcag
     1741 cgcgccgcgc gagagggtca ccgacacgac catgggcagc agctccggcg tcagtccgac
     1801 cgccagcgcc accgcgaaca tgaaggtctc ctgcaccggc cggttggcga gcaggtggac
     1861 gaacgcgatg aacagcacca gggccgtcgt catcctcagc acgagcaggc cgaagccgcg
     1921 cgtgccgcgc tcgaacgccg tcggcccggc cggctcgcgc aaggtgtcgg cgatgccgcc
     1981 gaggaaggtg gacggcccgg tgcgacacac gagcagcgtg gcgctgccgc tgagcacgga
     2041 cgatcccatg aaggcggcgt tcgtggcgtc gaccagcgac gtcgcgccgg cctcgacgtc
     2101 gccggcgtgc ttctcgaccg ggaaggactc gccggtgagc gccgactggc gcacgaagaa
     2161 gtcgcgtgcc tcgacgacgc ggccgtcggc cggcacgagg tcgcccgcgg cgaggcggac
     2221 gacgtcgccg ggcacgagct gcgcggcggg cagccgcgtc ggcgcgccgt cgcgcaggac
     2281 ggtcgcgcgc acggcgaccg aatcccgaag ccgcgtggcc gcggtcgcgg cgcggtgctc
     2341 ctgcacgacg tccagcgtca cgctgcccag gacgatcagg ccgatcaccg cgaagctggt
     2401 gacgtcgccc agcagcgccg aggccgcgct cgcggccagc agcagcagca ccagcgggtt
     2461 gcggaaatgc gagaggtact ccaccgccag cgcgcgccgg cgctcgaccc gcagcacgtt
     2521 gtccccgtgg accgcgcgca gcgaccgcgc ggcctcctcg ccgaggccgc gttcgctgct
     2581 gcgcagctcg tcccactgcc gctgcagcgg ccgcgtccag aacgcgtcga cgtccggcgc
     2641 cggctcgtgc atggcgatcc ccggcaggcc cggcgtcgtc ccgcgtcgct cagccgcccg
     2701 gcaacgcgcc gatgtcggcc aggtgcgcgc gccgctcgaa gaaccggcgc gcatgcgcgg
     2761 ccagctgcga ctgcgacagc tggtcgacga cgtcgttgcc gacgatccgg gcggccgtct
     2821 cgggccggtg cttgcggacg tagtgctcga agtcggcgag cgcctggtgg ccgcccacga
     2881 ccaggatctc gtcgacgtcg tcgaccgcgg cgcagacctc ggcgaggaat tcgtgcccgg
     2941 tgcgggcagc gctcgcgtgg cggcgcgtcg gatggcggtg ggcctccagc cggcgcgcca
     3001 gcggcgggtg cgaatccagc tgtagcacga gggcgtgcgc ctggtcgatg cggatcacgg
     3061 cgtgatggga catggcgttc ggcgcgacgg gcgcgcggtc gttggcggga atgacgggca
     3121 tcctgccgtc ggccgccgcg cgccgtcttg cgtcggcgca agcgtccgcg atcaggcggc
     3181 ccgcattgcg acggcgctgc cgtcgacggg cacgaggaat cgcgagtaca tgcgatgcgc
     3241 tccggtgccg atccacgcgg ggccggcgtg gacgacgagg ctcccgcccc agcggccggg
     3301 ccggtcatgg gaatcagtgc tcgaagagga ccggcatcgc cgcgccggcc gcgatcgagc
     3361 gcgtcgcgcc gccgagcacc cg
//
LOCUS       DASVLN010000957         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_373089, whole genome shotgun sequence.
ACCESSION   DASVLN010000957 DASVLN010000000
VERSION     DASVLN010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373089"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VF453_22465"
     CDS             <1..281
                     /locus_tag="VF453_22465"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7690484.1"
                     /translation="AEAEVVAGDLAAGIARLKSQPGRDILAHGGAGFARSLIAAGLVD
                     EYRLVVHPVVLGRGLPLFSELAQPRRLHRVATTAFPGGIVADVLRPAA"
     gene            298..954
                     /locus_tag="VF453_22470"
     CDS             298..954
                     /locus_tag="VF453_22470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7690485.1"
                     /translation="MVRGSPESKTKLLEAALHVIRAKGYAATTVDDICARAGLTKGSF
                     FHHFKSKDELALAAVEHFSAMAEGLFSNAPYLAHEDPLDRLLGYVDFRASMLDRDLPE
                     FTCLLGTLVQETYDTHPAIRSACDRGMSEHVDMLVRDVALAKARYAPDAAWTVDSLGY
                     FIQAALQGAFIFAKAKQGPAVARDCLAHLRRYLELLLQRDARSQRDSDRSEPARRQTP
                     "
     gene            951..1391
                     /locus_tag="VF453_22475"
     CDS             951..1391
                     /locus_tag="VF453_22475"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7690486.1"
                     /translation="MIAIASFVNVSVGDAARSDARRAPQRVGAVLSGLAVLFLLLDAV
                     VKLLALPVVLQTSAELGLPATPAFAQGLGVLLLACTLLYVWPRTAVVGAVLLTGYLGG
                     AIATHVRANNPLLTHVLFGLYVALFVWGGLYLRDPRVRALVGRR"
     gene            1494..1937
                     /locus_tag="VF453_22480"
     CDS             1494..1937
                     /locus_tag="VF453_22480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690487.1"
                     /translation="MSDDAPRYLHLVPNAPLPAAPFGGPFRAVVVVRRPVGDLWRKLA
                     SEWLVAGGCRYMLAWGHECSRWDDAVDWAALEANGFADAPPERFVMTTWHEDEPLEAV
                     FAYARRGARHPALAPGETWIVEVCDPWAASEPELRARWDAALDED"
     gene            complement(1956..>2185)
                     /locus_tag="VF453_22485"
     CDS             complement(1956..>2185)
                     /locus_tag="VF453_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690488.1"
                     /translation="YRQAWEALDAADPPDIASLRERALAATRQLAKRQVTWLRGMPAR
                     HVVEADAEDVESRLMATVERLLGPPGTAPGR"
BASE COUNT          248 a          834 c          786 g          317 t
ORIGIN      
        1 gggcggaggc cgaggtcgtc gcgggcgacc tggccgccgg catcgcgcgc ctgaagtcgc
       61 agcccggccg cgacatcctc gcccacgggg gcgccggctt cgcgcgcagc ctgatcgcgg
      121 cggggctggt cgacgagtac cggctggtcg tgcatcccgt cgtcctcggg cgcgggctgc
      181 cgctgttctc ggagctggcg cagccgcgcc gcctgcaccg cgtcgcgacg acggccttcc
      241 ccggcggcat cgtggccgac gtgctgcgtc cggcggcctg aagcgacgga gcgcgccatg
      301 gtccgcggtt ctcccgaatc gaagacgaag ctgctcgagg cggcgctgca cgtgatccgt
      361 gccaagggct acgccgcgac gacggtcgac gacatctgcg cccgcgccgg cctcacgaag
      421 ggcagcttct tccatcactt caagagcaag gacgagctcg cgctcgcggc cgtggagcat
      481 ttctccgcga tggccgaggg cctgttctcc aacgcgccct acctggcgca cgaggacccg
      541 ctcgaccgcc tgctcggcta cgtcgacttc cgcgcctcga tgctggaccg cgaccttccc
      601 gagttcacct gcctgctcgg cacgctggtg caggagacct acgacaccca ccccgccatc
      661 cgcagcgcct gcgatcgcgg gatgtccgag cacgtcgaca tgctggtgcg cgacgtcgcg
      721 ctggccaagg cgcgctacgc gcccgacgcc gcgtggacgg tcgacagcct cggctacttc
      781 atccaggccg cgctgcaggg cgcgttcatc ttcgccaagg ccaagcaggg gcctgcggtc
      841 gcgcgcgact gcctcgcgca cctgcgccgc tacctcgagc tgctgctgca gcgcgacgcc
      901 cgctcgcagc gggattccga ccgatccgaa cccgcaagga gacagacgcc atgatcgcca
      961 tcgcctcgtt cgtcaacgtg tccgtcggcg atgccgcccg cagcgatgcg cgcagggcgc
     1021 cgcagcgcgt cggcgccgtg ctctcgggcc ttgccgtgct gttcctgctg ctcgacgccg
     1081 tggtcaagct gctcgcgctg cccgtcgtgc tgcagacctc ggccgagctc ggcctgcccg
     1141 ccacgcccgc gttcgcgcag ggcctgggcg tgctgctgct cgcgtgcacg ctgctgtacg
     1201 tctggccgcg cacggcggtg gtcggcgcgg tgctgctgac cggctacctc ggcggcgcga
     1261 tcgcgacgca cgtgcgcgcg aacaacccgc tgctgacgca cgtgctgttc ggcctgtacg
     1321 tcgcgctgtt cgtctggggc gggctgtacc tgcgcgaccc gcgcgtgcgc gccctggtcg
     1381 gtcgccgctg aacgtccggg ccgcggcgcg gatccggaaa tacgtcgctt cgcggccgtc
     1441 cgcggcgccc gcggtcgcgt ccacgcgtgc gccaggggcc gtagactggc cccgtgtccg
     1501 acgatgcacc gcgctacctg cacctcgtcc cgaacgcgcc gctgcccgcg gcgcccttcg
     1561 ggggcccgtt ccgggccgtg gtggtcgtgc gccggccggt cggcgacctc tggcgcaagc
     1621 tcgccagcga gtggctcgtc gccggcggct gccgctacat gctggcctgg ggccacgagt
     1681 gcagccgctg ggacgacgcg gtcgactggg ccgcgctcga ggccaacggg ttcgcggacg
     1741 cgccgcccga acgtttcgtg atgacgacct ggcacgagga cgagccgctc gaggcggtct
     1801 tcgcctacgc ccggcgcggc gcgcggcacc cggcgctcgc tcccggcgag acctggatcg
     1861 tcgaggtctg cgatccctgg gcggcgtcgg agcccgagct gcgcgcgcga tgggacgcgg
     1921 cgctggacga ggattgactc gggcgcgaca cccgctcagc ggccgggcgc cgtcccgggc
     1981 gggccgagga gccgctcgac cgtcgccatc aggcggctct cgacgtcctc cgcgtcggcc
     2041 tcgaccacgt gccgcgccgg catgccgcgc agccaggtga cctgccgctt ggcgagctgc
     2101 cgcgtcgccg cgagggcccg ctcgcgcagg ctcgcgatgt cgggcggatc cgcggcatcg
     2161 agcgcctccc aggcctggcg gtagc
//
LOCUS       DASVLN010000958         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_373122, whole genome shotgun sequence.
ACCESSION   DASVLN010000958 DASVLN010000000
VERSION     DASVLN010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373122"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VF453_22490"
     CDS             <1..439
                     /locus_tag="VF453_22490"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7690489.1"
                     /translation="AAPGPAAARRAAFDPLIESVAQDYGQDADLLRAIVQVESGFDPA
                     AVSSRGAVGLMQVMPSTAADFGLAEPRSALLEPAVNLRVGAMLLRRLEQRFAGEPALV
                     IAAYNAGPEAVARAGGAIPPYAETQDYVRAVLAHWRALAQARR"
     gene            523..1632
                     /gene="sctU"
                     /locus_tag="VF453_22495"
     CDS             523..1632
                     /gene="sctU"
                     /locus_tag="VF453_22495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctU"
                     /protein_id="HEX7690490.1"
                     /translation="MSDDKTEEPTEHKLRKAREEGQMARSQDVAVAASTLFAVGALIG
                     TAPGIGTRLRAVVGLALDMPPDSAGMDVVYKRIGEMAFQAILAVLPIVVAASVGAAVG
                     MAAHVGFQITPKAMQPKFDNLNPASGIKKVFSLKSLLNFAQMLVKAVAIGIVMWQTIL
                     RLLPLVSGAVYETPDSVGTIAWKALVQLLLFGLAVFIVLAPVDFALQKHLFLKGQKMS
                     KDEVKREFKGQEGDPEVKGQRKQLARELAEESPHAAVGRADAVVVNPTHYAVAIRYRA
                     DEAGLPVVLAKGVDDAALRLRGIAAALGVPVFAHPPLARALHKVPVDHCVPEELFESV
                     AVVLRWVNEIGARREAGDEPGDAQEAGAAAPGSEP"
     gene            1629..>3626
                     /gene="sctV"
                     /locus_tag="VF453_22500"
     CDS             1629..>3626
                     /gene="sctV"
                     /locus_tag="VF453_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctV"
                     /protein_id="HEX7690491.1"
                     /translation="MKKPKIGGDAVLVATIVAILSLMVLPLPTFAIDTLLAVNLTIST
                     LLLMVTMYIPNAVALTSFPSLLLFTTLFRLSLNISSTKLILLHADAGHIIESFGNLVV
                     GGNLVVGIVVFLIITVVQFIVIAKGSERVAEVGARFTLDAMPGKQMSIDADLRAGHLS
                     AEEARQRRTMLAMESQLHGGMDGAMKFVKGDAIAALIITLINILAGIVIGVTYHGMTA
                     GEAAQRFAILSVGDAMVSQIPSLLICVAAGLMITRVADEDSPKRSLGDEITGQLTRNP
                     RALYLAAGFLCGFALVPGFPWYVFLILAGALAALGHQLSRRPAAADGNEAVDAMRGLR
                     REGAKARRGDIAAIEKRSGAFTAPLAVALAPGLGDALRPEALDAAFERERAGLQEQLG
                     LPFPGIRLWTRESCGDNEYEVLLHDVPFGRGTVVPGQLMLASPDAQQLEAASGSKPAG
                     GDERTCWFAPGSAPKDARMLSHEEVVARHAIRALQEQAHQFLGIQEVQWVMERVGAEY
                     PGLVAEMQKVVTPQRIAEVLRRLLEEQVPIRNMRAICESLVVWGPKEKDPLMLTEYVR
                     GDLGRFLSHRATQGSGSVCAVLLEPDIEKMIREAIKPTPAGNYLALGPEHVGAISRRV
                     RDLAGREPRPGVVVVTSMDIRRYVRRIVESESRWLSVYSYQE"
BASE COUNT          506 a         1267 c         1332 g          521 t
ORIGIN      
        1 ggcggccccg ggcccggcag ccgcccggcg cgcggcgttc gaccccctga tcgagtcggt
       61 ggcgcaggac tacggccagg acgccgacct gctgcgcgcc atcgtgcagg tggagtccgg
      121 cttcgatccg gcggccgtct cgtcccgggg cgccgtcggc ctgatgcagg tgatgccctc
      181 gaccgcggcg gacttcggcc tcgccgagcc gcgcagcgcg ctgctggagc ccgcggtcaa
      241 cctgcgcgtc ggcgcgatgc tgctgcgccg gctggaacaa cgcttcgcgg gcgagccggc
      301 gctcgtgatc gcggcctaca acgccggacc cgaagccgtc gcgcgcgccg gcggcgccat
      361 cccgccgtat gccgagacgc aggactacgt gcgggcggtg ctcgcgcact ggcgcgcgct
      421 cgcgcaggcc cgccgatgaa ccgcgcgctg cgtcggcttc gcgctcgccg cttcgcatgc
      481 gccggcgcac gcacgggcgg cggccgctag cctggagccg ccatgtccga cgacaagacc
      541 gaagagccga ccgagcacaa gctgcgcaag gcccgcgagg agggccagat ggcgcgcagc
      601 caggacgtcg ccgtcgcggc ctcgacgctg ttcgccgtcg gcgcgctgat cgggacggcg
      661 ccgggcatcg gcacgcgcct gcgcgccgtg gtcggcctcg cgctggacat gccgcccgac
      721 agcgccggca tggacgtggt ctacaagcgc atcggggaga tggcgttcca ggcgatcctc
      781 gcggtgctgc ccatcgtcgt cgccgcgtcg gtgggcgccg ccgtgggcat ggcggcgcat
      841 gtcggcttcc agatcacgcc caaggcgatg cagcccaagt tcgacaacct gaatcctgca
      901 tcgggcatca agaaggtctt ctcgctgaag tcgctgttga acttcgcgca gatgctcgtg
      961 aaggcggtgg cgatcgggat cgtgatgtgg cagacgatcc tgcggctgct tccgctcgtc
     1021 tccggcgccg tctacgagac gcccgactcg gtgggcacga tcgcctggaa ggcgctggtg
     1081 cagctgctgc tgttcgggct ggccgtcttc atcgtcctcg cgcccgtgga cttcgcgctg
     1141 cagaagcacc tgttcctcaa gggccagaag atgtccaagg acgaggtcaa gcgcgagttc
     1201 aagggccagg agggcgaccc cgaggtcaag ggccagcgca agcagctcgc gcgcgagctc
     1261 gccgaggagt ccccgcacgc ggccgtcgga cgcgcggacg cggtcgtcgt gaacccgacg
     1321 cactacgcgg tggcgatccg ctaccgcgcc gacgaggccg gcctgcccgt cgtgctggcc
     1381 aagggcgtcg acgacgccgc gctgcgactg cgcggcatcg ccgccgcgct gggcgtgccg
     1441 gtgttcgcgc atccgccgct ggcgcgcgcg ctccacaagg tgccggtcga tcactgcgtg
     1501 cccgaagagc tcttcgagtc cgtcgcggtc gtgctgcgct gggtcaacga gatcggcgcg
     1561 cgccgggagg cgggcgacga gcccggcgat gcgcaggaag cgggcgccgc ggcgccgggg
     1621 agcgaaccat gaagaagccc aagatcggcg gcgacgccgt cctggtggcc accatcgtcg
     1681 cgatcctgtc gctgatggtg ctgccgctgc ccaccttcgc gatcgacacg ctgctcgcgg
     1741 tgaacctcac gatcagcacc ctgctgctga tggtgacgat gtacatcccc aacgcggtcg
     1801 cgctcacctc cttcccgtcg ctgctgctgt tcacgacgct gttccggctg tcgctgaaca
     1861 tctcgtcgac gaagctgatc ctgctgcacg cggatgccgg ccacatcatc gagtccttcg
     1921 gcaacctggt cgtcggcggc aacctcgtgg tgggcatcgt ggtgttcctc atcatcacgg
     1981 tggtgcagtt catcgtgatc gccaagggct cggagcgcgt cgccgaggtc ggcgcgcgct
     2041 tcacgctcga tgcgatgccg ggcaagcaga tgagcatcga cgccgacctg cgcgcgggcc
     2101 acctgagcgc cgaggaggcg cgccagcgcc ggacgatgct ggccatggag agccagctgc
     2161 acggcggcat ggacggcgcg atgaagttcg tcaagggcga cgccatcgcc gcgctgatca
     2221 tcacgctgat caacatcctc gcgggcatcg tcatcggcgt gacgtaccac ggcatgaccg
     2281 cgggcgaggc cgcgcagcgc ttcgccatcc tctcggtggg cgacgccatg gtgtcgcaga
     2341 tcccgtcgct gctgatctgc gtggccgcgg gcctgatgat cacgcgcgtg gccgacgagg
     2401 actcgcccaa gcgctcgctc ggcgacgaga tcacggggca gctgacgcgc aacccgcgcg
     2461 cgctgtacct ggccgcgggc ttcctgtgcg ggttcgcgct ggtgccgggc ttcccctggt
     2521 acgtcttcct gatcctggcg ggcgcgctgg cggcgctcgg ccaccagctg tccaggcggc
     2581 ccgcggcggc ggacgggaac gaggccgtcg acgccatgcg ggggctgcgc cgagaggggg
     2641 cgaaggccag gcgcggcgac atcgcggcca tcgagaagcg cagcggcgcc ttcaccgcgc
     2701 cgctcgcggt ggcgctggcg ccgggcctcg gcgacgcgtt gcggccggag gcgctggacg
     2761 ccgccttcga gcgcgaacgc gccgggctgc aggagcagct gggcctgccc ttccccggca
     2821 tccggctgtg gacccgcgag tcgtgcggcg acaacgaata cgaggtgctg ctgcacgacg
     2881 tgccgttcgg ccgcggcacg gtcgtgcccg gacagctgat gctcgcatcg ccggacgcgc
     2941 agcagctgga agcggcgtcc ggatcgaagc ccgccggcgg ggacgagcgc acctgctggt
     3001 tcgcgcccgg ctcggcgccc aaggacgcgc ggatgctctc ccacgaggag gtcgtggcgc
     3061 gtcatgcgat ccgcgcgctg caggagcagg cgcaccagtt cctggggatc caggaagtgc
     3121 agtgggtgat ggagcgcgtc ggcgccgagt accccggcct ggtggcggag atgcagaagg
     3181 tggtcacccc gcagcgcatc gccgaggtgc tgcgccgcct gctggaggag caggtgccga
     3241 tccgcaacat gcgcgcgatc tgcgagagcc tcgtcgtctg gggacccaag gagaaggatc
     3301 cgctgatgct caccgagtac gtccgcggcg acctggggcg cttcctcagc catcgcgcga
     3361 cgcagggcag cggaagcgtg tgcgcggtgc tgctggagcc cgacatcgag aagatgatcc
     3421 gcgaggcgat caagccgacg ccggccggca actacctggc gctcgggccc gagcacgtgg
     3481 gcgcgatctc gcggcgcgtg cgcgacctcg ccgggcgcga gccgcggccg ggcgtcgtcg
     3541 tcgtgacgtc gatggacatc cgccgctacg tgcgccgcat cgtcgagtcc gagagccggt
     3601 ggctgtccgt ctactcctac caggag
//
LOCUS       DASVLN010000959         5879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_265757, whole genome shotgun sequence.
ACCESSION   DASVLN010000959 DASVLN010000000
VERSION     DASVLN010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265757"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VF453_22505"
     CDS             complement(<1..440)
                     /locus_tag="VF453_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7690492.1"
                     /translation="MQVITPKPSDLGDGFLVHRALPSAARRAVGPFVFLDQFGPVQLA
                     PGKGLDVRPHPHIGLATVSYLFEGEILHRDSLGSEQLIRPGEVNLMTAGRGIVHSERS
                     PASARAAGPRLWGLQFWVALPLALEDCAPAFAHHAADAIPRVARP"
     gene            514..1305
                     /locus_tag="VF453_22510"
     CDS             514..1305
                     /locus_tag="VF453_22510"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX7690493.1"
                     /translation="MADDEALLARIRRRLRLPPRRLVLWHTIAAVPMGTVVVLGAYLS
                     YHYHQLVIENRERVDHAYQVLDVVDGLYISLQNADVAQRDYIVTGDASRLQSFTAALR
                     EEERDAKRLQVLFEGAPKQSARLAQLDQAASAKLDELGRTITVRSRQGFDAAREIIRH
                     NDQGRAMDDIRRQVIAMSEAEHHLLMKRQTSLREHERNTLLVGVFIAALSIATRILIA
                     LGLNWYHRRQRRLHADIASGQVGKADRGDGDSEPEPAAGDEALAA"
     gene            complement(1391..2620)
                     /gene="alaC"
                     /locus_tag="VF453_22515"
     CDS             complement(1391..2620)
                     /gene="alaC"
                     /locus_tag="VF453_22515"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HEX7690494.1"
                     /translation="MSASAGKRRFARIDRLPPYVFNITAELKLAARRRGEDIIDMSMG
                     NPDGATPAHIVAKLTEVAQRPDTHGYSASKGIPRLRRAIARWYRERYAVEIDADKEAI
                     VTIGSKEGLAHLMLATLDRGDTVLVPDPSYPIHIYGAVIAGADIRSVPLVPGVDFFAE
                     LERAIRGSYPKPKMMVLGFPSNPTAQCVELDFFERVVALARKHDIFVVHDLAYADIVY
                     DGWKAPSIMQVQGAKDIAVEFFTLSKSYNMAGWRVGFMVGNPDLVAALARIKSYHDYG
                     TFTPLQVAAIAALEGDQSCVAEIAATYQSRRNVLHKGLNEIGWQVELPKASMYIWAPI
                     PAHYRHLGSLEFARQLLEKAKVCVSPGIGFGDHGDDHVRFALIENESRIRQALRGIRA
                     MFKADGVAPLVEAARAS"
     gene            2779..3519
                     /locus_tag="VF453_22520"
     CDS             2779..3519
                     /locus_tag="VF453_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEX7690495.1"
                     /translation="MRPIALACAVAVVLPAATLPAPARAGDAAIARFTTAAPGVPPAP
                     WKFVTLPRKAPTAFTIADVDGARALEVEANDSYGDLVHPLDVAPGAHPTIAWRWRVDR
                     LVDGADLRTRAGDDSPAKLCVSFAYDAAQLSFGERAKLALAHSATGQDVPAETLCYVW
                     DNRLAVDTAMANAFTRRIRFIVLQSGASRLGTWIAQKRDLGADYERLFGDESGGRMPA
                     ISGVEVSADADNTHGHALAWFGDVTLTP"
     gene            3712..4293
                     /locus_tag="VF453_22525"
     CDS             3712..4293
                     /locus_tag="VF453_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7690496.1"
                     /translation="MDKRSLVRALGACILAAGAASPALAADLTGKTVDIDWGFPTTSD
                     IFASDSVVVGAGPEVVCAGGGAGSGLCTGFIDAAHLDIGADTLSLTIDQGSAAWTGAA
                     FNGYEFTGLAAGGSWTGYSLSTDFSGLDSSRITFTPDAVFVNMQGIAPAAGQSFTITL
                     QAAAVPEPANAAMLLAGVAALGGLVRRQQARRR"
     gene            complement(4364..5869)
                     /locus_tag="VF453_22530"
     CDS             complement(4364..5869)
                     /locus_tag="VF453_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7690497.1"
                     /translation="MLPIFTVAVALSTFMEVLDTSIANVAVPTIAGNLAVSPNNGTWV
                     ISSYGLAAAIVVPLTGWIARRFGEVKTFVVSVLLFTLASALCGLATSLPMLVAFRFLQ
                     GLVSGPMVPLSQTLLLQNYPPAKRGMAMALWAMTVVIAPIFGPLLGGWITDNATWPWI
                     FYINAPIGVFAAFTTWTLLRHRDTPTVRQPIDVVGLLALLVGVGALQLMLDNGNDLDW
                     FNSPLITTLAIVAVVGLTFLVIWELTDAHPAVDLTLFRTRNFRFGTIAMALGYMAFFG
                     MTVLLPLWLQTVMGYTPTWAGIATAPVGLFALVFSPIVGRNAHRFDLRLGATLAFLVF
                     AGTAYWFSTFDLDTSLGHLVWPRLLQGVAVAFFFIPINQILLSGVQPQRMAAASGLSN
                     FFRTLSGSIGTAVVTTLWDHRTQVHQVRLAENFTPTGDAAQSYLGGLARAGVGGSAAD
                     AQVQNLIHGQSAMMATSEVFYGIAILFVALIALIWLTKPPFGVAGGAGGGH"
BASE COUNT          873 a         2067 c         2147 g          792 t
ORIGIN      
        1 gggcgcgcga cgcgcgggat cgcgtcggcc gcgtggtgcg cgaaggcagg cgcgcagtcc
       61 tcgagcgcga gcggcagcgc gacccagaac tgcagccccc acagccgcgg ccccgccgcg
      121 cgcgccgacg cgggcgaacg ctccgagtgc acgatcccgc ggccggccgt catcaggttg
      181 acctcgccag ggcggatcag ctgctcgctg ccgaggctgt cgcgatgcag gatctcgccc
      241 tcgaacaggt agctcaccgt cgccaggccg atgtgcggat gcgggcgcac gtccagcccc
      301 ttgcccggcg cgagctgcac cgggccgaac tggtcgagga agacgaacgg gccgaccgcg
      361 cggcgggcgg cgctgggcag cgcgcggtgg acgaggaagc cgtcgccgag gtcggacggc
      421 ttgggcgtga tcacttgcat gggcggctcc gcggcgagga agggcgcatg gtagcccgcg
      481 acggccgggc gcgtcgcttg ctctgcttca gccatggccg acgacgaggc gctcctcgcc
      541 cgcatccgcc gccgcctgcg cctgccgccg cggcggctgg tgctgtggca caccatcgca
      601 gccgtcccga tggggacggt cgtggtgctg ggcgcgtacc tgtcgtacca ctaccaccag
      661 ctcgtgatcg agaaccgcga gcgcgtcgac cacgcctacc aggtgctcga cgtcgtcgac
      721 ggcttgtaca tctcgctgca gaacgccgac gtcgcgcagc gcgactacat cgtcaccggc
      781 gacgcgagcc gcctgcaatc cttcaccgcg gccctgcgcg aggaggagcg cgacgcgaag
      841 cgcctgcagg tgctgttcga aggggcgccg aagcagagcg cgcgcctggc ccagctggac
      901 caggcggcgt ccgccaagct cgacgagctc ggtcgcacga tcaccgtgcg cagccggcag
      961 ggcttcgacg cggcgcgcga gatcatccgc cacaacgacc agggccgcgc gatggacgac
     1021 atccgccggc aggtcatcgc gatgtccgag gccgaacacc acctgctgat gaagcgccag
     1081 acctcgctgc gcgaacacga gcgcaacacc ctgctggtgg gcgtgttcat cgccgcgttg
     1141 tcgatcgcga cgcgcatcct catcgcgctc gggttgaact ggtaccaccg gcgccagcgc
     1201 cgcctgcatg ccgacatcgc cagcggtcag gtcggcaagg cggatcgcgg cgacggcgac
     1261 tcggagcccg agccggctgc gggggacgag gcactcgcgg cttgagggcc ggcggtgcgg
     1321 tcgcggcggg gccgggcatc ggcgctcccg ggcgtcccgg gttcggcccc gctccggcgc
     1381 ccggcgcgcg tcacgatgct cgcgcggcct cgacgagcgg cgcgacgccg tcggccttga
     1441 acatcgcgcg gatgccacgg agggcctgcc ggatgcgcga ctcgttctcg atcagcgcga
     1501 agcgcacgtg gtcgtcgccg tggtcgccga agccgatgcc gggcgagacg cagaccttgg
     1561 ccttctcgag cagctggcgc gcgaattcga gcgagccgag gtggcggtag tgcgcgggga
     1621 tgggcgccca gatgtacatc gaggccttgg gcagctcgac ctgccagccg atctcgttga
     1681 ggcccttgtg cagcacgttg cggcgcgact ggtaggtcgc cgcgatctcg gcgacgcagg
     1741 actggtcgcc ctccagcgcg gcgatcgcgg ccacctgcag cggcgtgaag gtgccgtagt
     1801 cgtggtagct cttgatgcgg gccagcgcgg ccacgaggtc ggggttgccg accatgaagc
     1861 cgacgcgcca gccggccatg ttgtagctct tgctcagggt gaagaactcc accgcgatgt
     1921 ccttggcgcc ctgcacctgc atgatggacg gcgccttcca gccgtcgtag acgatgtcgg
     1981 cgtaggcgag gtcgtggacg acgaagatgt cgtgcttgcg cgccagcgcg acgacgcgct
     2041 cgaagaagtc gagctccacg cactgcgccg tcgggttcga cgggaagccg agcaccatca
     2101 tcttcggctt cgggtagctg ccgcggatcg cgcgctcgag ttcggcgaag aagtccacgc
     2161 cgggcaccag cggcaccgag cggatgtccg cgcccgcgat caccgcgccg tagatgtgga
     2221 tcgggtagct cgggtcgggc acgagcaccg tgtcgccgcg gtccagcgtc gcgagcatca
     2281 ggtgcgcgag gccttccttc gagccgatgg tgacgatggc ctccttgtcg gcatcgatct
     2341 cgaccgcata ccgctcccgg taccagcgcg cgatcgcgcg gcgcaggcgc gggatgccct
     2401 tggacgccga gtagccgtgc gtgtcgggcc gctgggcgac ctcggtgagc ttggcgacga
     2461 tgtgcgcggg cgtggccccg tcggggttgc ccatgctcat gtcgatgatg tcttcccctc
     2521 gcctgcgggc ggcgagcttg agctccgccg tgatgttgaa gacgtaggga gggaggcggt
     2581 cgatgcgcgc gaagcggcgc ttgcctgcgg aagcggacat ggagactttc acgtgagcgc
     2641 ccggaaccgt ccgagcgacg tcggccgggg ggccggcctg cgacgcatga tagggattcg
     2701 cgcgccggct taccatcgcg ggctcgcggc gtgtcgcgcg gatgcgtcgc cgctccctca
     2761 tcccttcgag gatcccgcat gcgcccgatc gcgttggcct gcgccgtcgc cgttgtcctg
     2821 cccgccgcca ccctcccggc gcctgcccgt gccggcgacg ccgcgatcgc gcggttcacg
     2881 accgccgcgc ccggcgtgcc gcccgcgccc tggaagttcg tcacgctgcc gcgcaaggcg
     2941 ccgaccgcct tcacgatcgc cgacgtcgat ggcgcgcgcg cgctcgaggt ggaggcgaac
     3001 gactcgtatg gcgacctcgt gcacccgctg gacgtcgcgc ccggcgcgca tccgacgatc
     3061 gcctggcgct ggcgcgtcga ccggctggtg gacggcgccg acctgcgcac gcgcgcgggc
     3121 gacgactcgc cggccaagct gtgcgtgtcc ttcgcgtacg acgcggcgca gctgtccttc
     3181 ggcgagcgcg ccaagctggc gctcgcgcat tcggccaccg gccaggacgt gcccgccgag
     3241 accctgtgct acgtctggga caaccggctg gcggtcgaca ccgcgatggc caacgcgttc
     3301 acgcggcgca tccgcttcat cgtcctgcag agcggcgcgt cgcggctcgg cacgtggatc
     3361 gcgcagaagc gcgacctcgg cgcagactac gagcgcctgt tcggcgacga gtccggcggg
     3421 cgcatgcccg cgatctcggg cgtcgaggtg tccgcggacg ccgacaacac gcatggccac
     3481 gcgctcgcgt ggttcggcga cgtcacgctg acgccctgac gcgtcgaccg cctcgactcg
     3541 cggtgctgcg cgacgcgcag gcgcgaggca agcaccgggc cgtccctagc tcgtggtgga
     3601 tccgcacgaa tgcgggatgg gcgccggcgc aggccgtgtg gacactgcgc gccagccccg
     3661 gtgcctccgg agcgacgcga ggcccgcgac aaggccgcaa ggagtgaacc catggacaag
     3721 cgatccctcg tacgcgcgct cggcgcgtgc atcctcgccg cgggcgcggc gagcccggcc
     3781 ctcgcggccg acctgacggg caagaccgtc gacatcgact ggggcttccc gaccacgagc
     3841 gacatcttcg ccagcgactc ggtcgtcgtg ggcgcgggcc ccgaggtcgt gtgcgccggc
     3901 ggcggcgcgg gcagcggcct gtgcaccggc ttcatcgatg cggcgcacct ggacatcggc
     3961 gccgacacgc tgtcgctgac catcgaccag ggctcggccg cgtggaccgg cgccgcgttc
     4021 aacggctacg agttcaccgg cctcgccgcc ggcggcagct ggaccggcta ctcgctgtcg
     4081 accgacttct ccggtctcga cagctcgcgc atcacgttca cgcccgacgc cgtgttcgtg
     4141 aacatgcagg gcatcgcgcc cgcggccggc cagtccttca cgatcaccct gcaggccgcg
     4201 gccgtccccg agcccgcgaa cgcggccatg ctgctagccg gcgtcgcggc gctgggcggg
     4261 ctggtgcgcc ggcagcaggc gcggcggcgc tgagcagggg catcgtcggg atggacgaag
     4321 ggccggcatc gcgccggccc ttcgcctccg ccgacgcggt gagtcagtgc ccgccgcccg
     4381 cgcctccagc caccccgaac ggcggcttcg tcagccagat cagcgcgatc agcgcgacga
     4441 acaggatcgc gatgccgtag aacacctcgc tcgtggccat catcgcggac tggccgtgga
     4501 tcaggttctg cacctgcgca tcggccgcgc tcccgccgac gccggcgcgc gcgaggccgc
     4561 cgaggtagga ctgcgcggcg tcgccggtgg gcgtgaagtt ctcggccagg cgcacctggt
     4621 ggacctgcgt gcggtggtcc cacagcgtcg tcacgaccgc ggtgccgatg ctgcccgaga
     4681 gcgtgcggaa gaagttcgac agccccgagg ccgcggccat gcgctgcggc tgcacgcccg
     4741 acagcaggat ctggttgatc gggatgaaga agaacgccac cgcgacgccc tgcagcaggc
     4801 gcggccagac gaggtggccg agcgaggtgt cgaggtcgaa ggtcgagaac cagtacgcgg
     4861 tgccggcgaa cacgaggaac gcgagcgtcg cgcccaggcg caggtcgaag cggtgcgcgt
     4921 tgcggccgac gatgggcgag aacacgagcg cgaacagccc caccggcgcg gtcgcgatgc
     4981 cggcccaggt gggcgtgtag cccatcaccg tctgcagcca cagcggcagc agcacggtca
     5041 tgccgaagaa ggccatgtag ccgagcgcca tcgcgatcgt gccgaagcgg aagttgcgcg
     5101 tcctgaacag cgtcaggtcg accgccggat gggcgtcggt caactcccag atgacgagga
     5161 aggtgaggcc gaccacggcg acgatcgcga gcgtcgtgat caacggcgag ttgaaccagt
     5221 cgaggtcgtt gccgttgtcc agcatcagct gcagcgcgcc gacgcccacc aggagcgcga
     5281 gcaggccgac gacgtcgatc ggctggcgca ccgtcggcgt gtcgcggtgt cgcagcagcg
     5341 tccaggtcgt gaaggccgcg aacacgccga tcggcgcgtt gatgtagaag atccacggcc
     5401 aggtggcgtt gtcggtgatc cagccgccca gcagcgggcc gaagatcggc gcgatcacga
     5461 ccgtcatcgc ccacagcgcc atcgccatgc cgcgcttggc gggcggatag ttctgcagca
     5521 gcagcgtctg cgacagcggc accatcgggc ccgagaccag gccctgcagg aatcggaacg
     5581 ccaccagcat cggcaggctc gtcgcgaggc cgcacagtgc cgacgcgagc gtgaacagca
     5641 gcaccgagac gacgaaggtc ttcacctcgc cgaagcggcg cgcgatccag ccggtcagcg
     5701 gcacgacgat cgccgcggcc aggccgtagg acgagatgac ccaggtgccg ttgttggggc
     5761 tcaccgccag gttgccggcg atcgtcggca cggcgacgtt ggcgatcgag gtgtcgagca
     5821 cctccatgaa cgtcgacagg gcgaccgcga ccgtgaagat gggcagcagg tacgcgggc
//
LOCUS       DASVLN010000960         8993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_211608, whole genome shotgun sequence.
ACCESSION   DASVLN010000960 DASVLN010000000
VERSION     DASVLN010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211608"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF453_22535"
     CDS             complement(<1..387)
                     /locus_tag="VF453_22535"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEX7690498.1"
                     /translation="MDALSRLVTPEILVPVAGMLMPIAIVLIALVYAQRFQERKHQTI
                     VGLLEKGLPVPRELLRPARSGGSALMRAMTLVGAGLGICGFLGAMFGPSRGLWASGLV
                     PLCIGLAQLVALKFEPARPDVDPPSAI"
     gene            718..1998
                     /locus_tag="VF453_22540"
     CDS             718..1998
                     /locus_tag="VF453_22540"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 18 protein"
                     /protein_id="HEX7690499.1"
                     /translation="MRRFLLASVLAACGLAASAVPRAPGPAGAPYPLFGGKPLEFERS
                     GGKIVGVYVPNWEPPALVDALHGDSVTHVVYAFLHACGPGQLAMDAPKCQGRPDFTLG
                     WDGVDATFDAAFQRLKARAPHVRVLASVGGWGGSDPFFHIANDPAARAIFAKSAAEFL
                     RTHPGFDGIDIDWEHPTTNGSANGVALGSPADGQGYADLMAALRSALDTLTAEIGHKY
                     LLTSAVNTSHAIVDKVNFRDAARSLDYVFMMTYDFYGSWSKAAGNHATPRASAPGAED
                     GLDAATKAMLAAGVPAGKLVAGVAMYGRGFTGVAPARDGVSFTGSAFTASYPAPEGSS
                     SWRELHARYLDARGRGRHGYKAVFDPVTRGWSLYNARNRLFMGYDDPRAVIEKGAFVR
                     ANGLAGVFAWELSQDDGDILNAMNWGVGNQLLQP"
     gene            complement(1952..2920)
                     /locus_tag="VF453_22545"
     CDS             complement(1952..2920)
                     /locus_tag="VF453_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7690500.1"
                     /translation="MDKLKAMRTFVQIVDDGSLTAAAASLGSSLPAVVRGLAALEAEL
                     GTRLFQRTTRRVALTEEGRHYAARCRDVLAAVAAADASLTDAQSQPRGRLAITAPVLL
                     GQHVVADLVSRFVAAHPQVRCSVLLVDRYVNLVEEGLDVAIRIGALDDSSLVATRLGE
                     VRHVVVASPAFLRRHGTPRHPSELRDRPCVRLSGPVRPGWAFQDGGRRLHVAVDGPLD
                     FNHAGAALRACGNGLGFGQFFSYQVQDAVREKRLRIVLEDFEPARLPVSVVWPNARGL
                     PARTRAFVDAAQRELAPLFGRRGERSDDRVAVRAAATGCPRPSSSR"
     gene            3036..3746
                     /locus_tag="VF453_22550"
     CDS             3036..3746
                     /locus_tag="VF453_22550"
                     /inference="COORDINATES: protein motif:HMM:NF004836.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690501.1"
                     /translation="MTRPIRIAIVGDRDDAIAAHRAIPIALADVAAAHDLELAVDWLA
                     TDAVGDGRALARFDGLWCAPGSPYRSEDGALRAIAHARTTGTPFLGTCGGFQHALLEF
                     ARAVLGWDDATHAESEPGVGRAVIAPLECALVEASGFATLAPGSRIAKAYGATTAPTI
                     YRCRYGLAPEFRAALTAGPLRATAEDERGALRAVELDDHPFFVATLWQPERAALALQP
                     VPLAQAFAAAAHAYAAAR"
     gene            3767..4243
                     /locus_tag="VF453_22555"
     CDS             3767..4243
                     /locus_tag="VF453_22555"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7690502.1"
                     /translation="MSHCLHTATIRWQRGDARFSDRRYSRAHAWSFDGGLAVRASSSP
                     HVVPVPMSDASAVDPEEAFVAALASCHMLWFLDVAGRAGYVVDDYEDAAEGAMGRDAA
                     GREVVATVVLRPRTRFSGDRRPDAAALDALHHAAHEACFLANSVRSEIRCEPALVD"
     gene            complement(4314..5378)
                     /locus_tag="VF453_22560"
     CDS             complement(4314..5378)
                     /locus_tag="VF453_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7690503.1"
                     /translation="MGALTIANVRKTFGNVDILKGIDIDIEAGEFLILVGPSGCGKST
                     LLNIIAGLEMPTGGSIKIGERDVTWLPPKDRDIAMVFQSYALYPNMNVAQNIAFGLEM
                     RKVPKDKREEAVQRVAKMLQITHLLDRKPGQLSGGQRQRVAMGRALARDPKLFLFDEP
                     LSNLDAKLRVEMRAEIKLLHQRTRVTTVYVTHDQVEAMTLGDRIAVMKEGVVQQFGTP
                     EDIYTRPATKFVAEFIGSPAMNMIPAVRSGGGVAANGVELALSAAQRAALQQGAASGL
                     IYGLRPEAIAFAEDGLPGKVHFTEPTGPETYATVETAIGMLTLRVPGVLHAKVGDAVH
                     VKWSADAVHLFDASSDKRIA"
     gene            complement(5383..6168)
                     /locus_tag="VF453_22565"
     CDS             complement(5383..6168)
                     /locus_tag="VF453_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7690504.1"
                     /translation="MPLYVMLVTSLKDMNQIRSGNLMSLPASLNFASWAKAWSEACTG
                     TDCSGLKPYFMNSVMMVVPAVAISTSLGIVNGYVLSKWRFRGSEVMFAMMLFGVFMPM
                     QVVLLPMSQVLGYFGIANSIWGLIVMHVLAGIPSTTLFFRNYYIGLPDELMKAAMLDG
                     AGFWRILWRVVLPLSTPILVVTLIWQFTNIWNDFLYGIVFSGADSKPVTAGLNNLANT
                     TSSVKEYNVDMAAALIAALPTLFVYVVAGKYFVRGLTAGAVKG"
     gene            complement(6243..7121)
                     /locus_tag="VF453_22570"
     CDS             complement(6243..7121)
                     /locus_tag="VF453_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7690505.1"
                     /translation="MTRSQAPRTAWLPKLVVAPSFLLSFFFIYGLIAWNGYLSLSASH
                     ILPNYTWVGIQNYLDLFDNDRFHVAIVNMGIFGGLFIGGSMALGLLLAILLDQKIRGE
                     GVLRTVYLYPMAISFIVTGTAWKWILNPGLGIEHLVRSWGFPNFTFDWLVDDQHAIYC
                     IVIAGIWQASGFVMALFLAGLRGIDDSIIKAAQVDGASLPRIYWRIVVPSLRPVFFST
                     LMVVSHLAIKSFDLVIALTAGGPGYSTDLPATFMYAMSFSRGQIAIGAASAEIMLATV
                     AAIVVPYLYSELRTKK"
     gene            complement(7339..8592)
                     /locus_tag="VF453_22575"
     CDS             complement(7339..8592)
                     /locus_tag="VF453_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7690506.1"
                     /translation="MSKSFPRLTVVALAAILGAGVAHADELEVLHWWTSGGEAKAAAE
                     LKKEMEAEGHTWKDFAVAGGGGDSAMTVLKSRVISGNAPAAAQIKGPALQEWAAEGVL
                     ANIDDVAKAEKWDSVLPPVVANVMKYKGHYIAAPVNVHRVNWLWANPEAFKKAGAKVP
                     TTWDEFFAAGDKLKAAGIIPLAHGGQNWQDFTVFEDVALGVGGPDFYRKALVQLDPAT
                     INGPTMIKVLDTFKKVHGYIDKNSPGRDWNLATAMVIKGEAGMQLMGDWAKGEFLAAG
                     KKPGTDFVCTPAPGTAKAYTFNIDSFAMFQLKNPANEKAQKDLAKAIMSPHFQELFNL
                     NKGSIPVRLDVKLDKFDECAKESNKDFVATSKANTLLPSIAHGMAVPPAAEGAIKDAV
                     SKYWNDDKETSKDAQADILKASKAK"
     gene            complement(8672..>8993)
                     /locus_tag="VF453_22580"
     CDS             complement(8672..>8993)
                     /locus_tag="VF453_22580"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX7690507.1"
                     /translation="STSPSSNFWTIGGRGSYALTKNVKWLTEIGYSSLKPSGGPSENV
                     TKVTVGPALSTGPDFWKRPELRLYVTYADYNKAAAQDTYNNYGFRPDKTNAVSYGAQV
                     EIWF"
BASE COUNT         1363 a         3134 c         3135 g         1361 t
ORIGIN      
        1 gatggccgac ggcgggtcga cgtcgggccg cgcgggctcg aacttcagcg cgaccagttg
       61 cgccagcccg atgcacaacg ggaccaggcc ggacgcccac aacccgcgcg aggggccgaa
      121 catcgcgccc aggaagccgc agatgcccag gcccgccccc accagcgtca tcgcccgcat
      181 cagtgcggat ccgcccgaac gcgccggccg cagcagctcg cgcggcaccg gcaggccctt
      241 ctccagcagg ccgacgatgg tctggtgctt gcgctcctgg aaacgctggg cgtagacgag
      301 cgcgatcagg acgatggcga tcggcatcag catcccggcg acggggacga ggatctcggg
      361 cgtgacgagg cgggacaggg catccatggc gggtctccag gcggacgatg aagcgggtct
      421 tcctgcttgg atgcgtggaa aggcgcgatc ggatgcaggc ggcgcgaaat ctttcgtgcc
      481 cggcgcggcg gcatgtccgg cggcggttcg ccccggaggc gactatcccg gcccgtcgca
      541 ccgcccgcaa gcgggatgac gcgaacgcgc cgacgcttcc cgctgggcga tctgctcgat
      601 tggctttata gtggtactaa agtggttcgc gcgcagcgcg cgccggatcg ccgaccgccc
      661 tcgtttccgc cacaccgccc catgacgccc tcgacgcctc gccgatccgc cgccgccttg
      721 cgccgcttcc ttctcgccag cgtgctcgcg gcctgtggcc tggctgcgtc cgcagtgccg
      781 cgcgcgcccg ggccggccgg cgcaccgtat ccgttgttcg gcggcaagcc gctcgagttc
      841 gagcgcagcg gcggcaagat cgtcggcgtc tacgtgccga actgggaacc gccggcgctc
      901 gtcgacgcgc tgcatggcga cagcgtcacc cacgtggtct acgcgttcct gcatgcctgc
      961 gggccgggac aactggcgat ggacgcgccg aagtgccagg gccggcccga cttcacgctc
     1021 ggctgggatg gcgtcgacgc gaccttcgac gccgcgttcc agcggctgaa ggcgcgggcg
     1081 ccgcacgtgc gggtgctcgc gtcggtcggc ggctggggcg ggtcggatcc gttcttccac
     1141 atcgccaacg acccggccgc ccgcgccatc ttcgccaagt ctgccgccga gttcctgcgc
     1201 acgcatcccg gcttcgacgg catcgacatc gactgggagc atccgaccac caacggctcg
     1261 gccaacggcg tcgcgctcgg ctcgcccgcc gacggccagg gctacgccga cctgatggcg
     1321 gcgctgcgct cggccctgga cacgctcacc gccgagatcg gccacaagta cctgctcacg
     1381 agcgcggtca acacctcgca cgcgatcgtc gacaaggtga acttccgcga tgccgcgcgc
     1441 tcgctggact acgtcttcat gatgacctac gacttctacg gcagctggag caaggccgcg
     1501 ggcaaccatg cgacgccgcg cgcgagcgcc cccggcgccg aggacggcct ggacgccgcg
     1561 acgaaggcga tgctcgcggc gggcgttcca gcgggcaagc tggtcgccgg cgtggcgatg
     1621 tacgggcgcg gcttcaccgg cgtggcgccc gcgcgcgacg gcgtgtcctt cacgggctcg
     1681 gcgttcacgg ccagctatcc cgcgcccgag ggctcgagca gctggcgcga gctgcacgcg
     1741 cgctatctcg acgcgcgggg ccgcggacgc cacggctaca aggcggtgtt cgatccggtc
     1801 acgcgcggct ggagcctcta caacgcccgc aaccgcctgt tcatgggcta cgacgacccg
     1861 cgcgccgtga tcgagaaggg cgccttcgtg cgcgccaacg gcctggcggg cgtgttcgcg
     1921 tgggagctgt cgcaggatga cggcgacatc ctcaacgcga tgaactgggg cgtgggcaac
     1981 cagttgctgc agccctgacg gccactcggt cgtccgacct ctcgccgcgt cgcccgaaca
     2041 gcggcgcgag ctcgcgctgc gccgcgtcga cgaacgcacg cgtgcgcgcc ggcaggcccc
     2101 gtgcgttcgg ccagacgacg ctgaccggca gccgcgcggg ctcgaagtcc tcgagcacga
     2161 tccgcagccg cttctcgcgc acggcgtcct gcacctggta cgagaagaac tggccgaagc
     2221 ccaggccgtt gccgcaagcg cgcagcgccg cgccggcatg gttgaagtcg agcgggccgt
     2281 cgacggcgac gtgcaggcgc cggccgccgt cctggaacgc ccagccgggc cgcaccggcc
     2341 ccgacagtcg cacgcacggc cggtcgcgca gctccgacgg atggcgcggc gtgccatggc
     2401 ggcgcaggaa ggccgggctc gcgaccacca cgtggcgcac ctcgcccagc cgcgtcgcaa
     2461 cgagcgagga atcgtcgagc gcgccgatgc ggatcgcgac gtccagccct tcctcgacga
     2521 ggttgacgta gcggtcgacc agcagcacgc tgcagcgcac ctgcggatgc gcggcgacga
     2581 agcgcgagac gaggtcggcc accacgtgct ggccgagcag cacgggcgcc gtgatcgcga
     2641 gccggccgcg cggctgcgac tgggcgtcgg tcaggctcgc gtcggccgcg gcgaccgcgg
     2701 ccagcacgtc gcggcaacgc gcggcgtagt ggcggccctc ctcggtgagc gcgacgcggc
     2761 gcgtggtgcg ctggaacagc cgcgtgccga gctccgcctc cagcgccgcc agcccgcgca
     2821 cgaccgccgg cagcgaggag cccagcgacg ccgccgccgc cgtgaggctg ccgtcgtcga
     2881 cgatctgcac gaaggtccgc atcgccttga gcttgtccat ggcagcggat attaatccgc
     2941 aaggcgcagc aaagaactga tcgagctggc atttactgcg aaacgcgtgc ggacgatcat
     3001 cccggctgtc cccactccgc ccgccgaagg cccgcatgac ccgacccatc cgcatcgcca
     3061 tcgtgggcga tcgcgacgac gcgatcgccg cccaccgcgc cattcccatc gcgctcgccg
     3121 acgtggcggc cgcgcacgac ctcgagctgg ccgtcgactg gctcgccacc gacgccgtcg
     3181 gcgacggccg cgcgctcgcc cgcttcgatg gactctggtg cgcacccggc agcccgtacc
     3241 gcagcgagga cggggcgctg cgcgcgatcg cgcacgcgcg caccacgggc acgcccttcc
     3301 tcggcacctg cggcggcttc cagcacgcgc tgctggaatt cgcgcgcgcc gtcctcggct
     3361 gggacgacgc cacgcatgcc gagagcgagc ccggcgtcgg ccgcgccgtc atcgcgccgc
     3421 tggaatgcgc actggtcgag gcctcgggct tcgcgacgct ggcgcccggc tcgcgcatcg
     3481 cgaaggcata cggcgcgacg acggcgccga cgatctaccg ctgccgctac gggctcgcgc
     3541 cggagttccg cgccgcgctg accgccggcc cgctgcgcgc cacggccgag gacgagcgtg
     3601 gcgcgctgcg tgccgtcgag ctcgacgacc accccttctt cgtcgccacg ctgtggcagc
     3661 ccgagcgcgc cgccctggcg ctgcagccgg tgccgctcgc gcaggccttc gccgccgcgg
     3721 cgcatgcgta cgccgccgcc cgctgatcga caggaccccc gaccccatga gccattgcct
     3781 gcacaccgcc accatccgct ggcagcgtgg tgacgcgcgc ttctccgacc gccgctactc
     3841 gcgcgcgcac gcctggtcgt tcgacggcgg gctcgccgtg cgggcgtcgt cctcgccgca
     3901 cgtcgtgccg gtgccgatgt cggacgcctc cgccgtcgac cccgaggagg ccttcgtcgc
     3961 cgcgctggcg agctgccaca tgctgtggtt cctcgacgtc gcgggacgcg ccggctacgt
     4021 cgtcgacgac tacgaggacg ccgccgaagg cgcgatgggc cgcgacgccg cgggccgcga
     4081 ggtcgtcgcg accgtggtgc tgcgcccgcg cacgcgcttc tccggcgacc gccggcccga
     4141 tgccgccgcg ctcgacgcac tgcaccacgc ggcccacgag gcctgcttcc tcgcgaactc
     4201 ggtgcgcagc gagatccgct gcgagccggc gctggtcgac tgagcgccgg cgccgaaacg
     4261 acaacggggc atggcgcgcg ccatgccccg tgccgtatcg aaggcccgtc ggatcaggcg
     4321 atgcgcttgt cgctcgacgc gtcgaagagg tgcaccgcgt cggccgacca cttcacgtgc
     4381 accgcgtcgc cgaccttggc gtgcagcacg ccgggcacgc gcagcgtcag catgccgatc
     4441 gccgtctcga cggtggcgta ggtctcgggg ccggtgggct cggtgaaatg caccttgccc
     4501 ggcagcccgt cctcggcgaa ggcgatcgcc tccgggcgca ggccgtagat cagccccgag
     4561 gccgcgccct gctgcagcgc ggcgcgctgg gccgcgctga gcgcgagctc gacgccgttg
     4621 gccgcgacgc cgccgcccga gcgcaccgcc ggaatcatgt tcatggccgg cgagccgatg
     4681 aactcggcga cgaacttcgt ggccggacgc gtgtagatgt cctcgggcgt gccgaactgc
     4741 tgcacgacgc cttccttcat cacggcgatg cggtcgccga gcgtcatcgc ctcgacctgg
     4801 tcgtgcgtca cgtagaccgt ggtcacgcgc gtgcgctggt ggagcagctt gatctcggcg
     4861 cgcatctcga cgcgcagctt ggcgtcgagg ttggacagcg gctcgtcgaa caggaacagc
     4921 ttcgggtcgc gcgcgagcgc ccggcccatc gccacgcgct ggcgctggcc gcccgagagc
     4981 tggcccggct tgcgatcgag caggtgcgtg atctgcagca tcttggccac gcgctgcacg
     5041 gcctcctcgc gcttgtcctt gggcaccttg cgcatctcga ggccgaacgc gatgttctgc
     5101 gccacgttca tgttcgggta gagcgcgtag ctctggaaca ccatcgcgat gtcgcgatcc
     5161 ttgggcggca gccaggtgac gtcccgctcg ccgatcttga tcgagccgcc ggtgggcatc
     5221 tcgagccccg cgatgatgtt gagcagcgtg gacttgccgc agcccgaggg cccgaccagg
     5281 atcaggaatt cccccgcctc gatgtcgatg tcgatcccct tgaggatgtc gacgttcccg
     5341 aaagtcttgc gtacgttggc gatcgtcagc gcgcccatct cgttatccct tcaccgcgcc
     5401 ggccgtcagg ccgcgcacga aatacttgcc agcaaccacg tagacgaaca gcgtcggcag
     5461 cgcggcgatc agtgccgcgg ccatgtccac gttgtattcc ttcaccgagc tcgtcgtgtt
     5521 cgcgaggttg ttcaggccgg cggtgaccgg cttggagtcc gcgcccgaga acacgatccc
     5581 gtacaggaaa tcgttccaga tgttcgtgaa ctgccagatc agcgtcacga ccaggatcgg
     5641 cgtcgacagc ggcagcacga cccgccacag gatgcgccag aagccggcgc cgtccagcat
     5701 ggccgccttc atcagctcgt cgggcaggcc gatgtagtag ttgcggaaga acagcgtcgt
     5761 cgacgggatg cccgcgagca cgtgcatcac gatcaggccc cagatcgagt tggcgatgcc
     5821 gaagtagccg agcacctggc tcatcggcag cagcacgacc tgcatcggca tgaacacgcc
     5881 gaacagcatc atcgcgaaca tcacctcgct gccccggaag cgccacttcg acagcacata
     5941 gccgttgacg atgcccagcg aagtcgagat ggcgaccgcg ggcacgacca tcatcacgct
     6001 gttcatgaag tacggcttca ggccgctgca gtcggtgccg gtgcaggcct cgctccaggc
     6061 cttggcccac gacgcgaagt tgagcgaggc gggcagcgac atcaggttgc cgctgcggat
     6121 ctggttcatg tccttcagcg acgtcacgag catcacgtac aagggcatca ggaaccaggc
     6181 cgcgaacacc agcagcgcgg tccacaggac gacgcggtgc caggtcaggg agctgggcgg
     6241 cttcacttct tggtcctcag ttcggagtac aggtagggca cgacgatggc ggccaccgtc
     6301 gcgagcatga tctcggcgct ggcggcgccg atggcgatct ggccgcgcga gaacgacatc
     6361 gcgtacatga aggtcgcggg caggtcggtg gagtagcccg ggccgccggc ggtcagcgcg
     6421 atcacgaggt cgaagctctt gatcgcgaga tggctcacca ccatcagcgt gctgaagaac
     6481 accggccgca gcgacggcac gacgatgcgc cagtagatgc gcggcagcga ggcgccgtcc
     6541 acctgcgcgg ccttgatgat ggagtcgtcg atgccgcgca ggccggcaag gaacagcgcc
     6601 atcacgaagc ccgaggcctg ccagatgccg gcgatcacga tgcagtagat cgcgtgctgg
     6661 tcgtccacga gccagtcgaa ggtgaagttg gggaagcccc agcttcgaac gaggtgctcg
     6721 atgcccaggc ccgggttcag gatccacttc caggccgtgc cggtgacgat gaacgagatc
     6781 gccatcgggt acaggtacac ggtacgcagg acgccttcgc cgcggatctt ctgatcgagc
     6841 aggatcgcca gcagcaggcc gagcgccatc gagccgccga tgaacaggcc gccgaagatg
     6901 cccatgttga cgatggcgac gtggaagcgg tcgttgtcga agaggtcgag gtagttctgg
     6961 atgccgaccc acgtgtagtt cggcaggatg tgcgacgcgg acagcgacag gtagccgttc
     7021 caggcgatca gcccgtagat gaagaagaac gacagcagga agctgggcgc gaccaccagc
     7081 ttcggcagcc aggccgtgcg tggggcttgg gagcgtgtca tacgcgagcg gtgctttctg
     7141 atggagcgag cggtgcggac agcgtagcag cggggcacgc ggctcgcacg ggggtcagtc
     7201 cggggtccga ccgggggcaa acggggcgcc atggccgggg aggccggtcg ccgggcagaa
     7261 aaagcgccac gcgcgtgctg ccgcgcgtgg cgaatgccca atggagatat gcgtcgccgg
     7321 gacgcaggga aagccggatt acttggcctt ggacgccttc aggatgtcgg cctgcgcgtc
     7381 cttgctcgtt tccttgtcgt cgttccagta cttgctgacg gcgtccttga tcgcgccttc
     7441 cgcagccggc gggacggcca tgccgtgggc gatcgagggc agcagcgtgt tcgccttcga
     7501 ggtcgcgacg aagtccttgt tggactcctt ggcgcactcg tcgaacttgt ccagcttgac
     7561 gtcgaggcgg accgggatcg agcccttgtt caggttgaac agctcctgga agtgcggcga
     7621 catgatggcc ttggcgaggt ccttctgtgc cttctcgttg gccgggttct tcagctggaa
     7681 catcgcgaac gagtcgatgt tgaaggtgta ggccttcgcg gtgcccgggg ccggggtgca
     7741 gacgaagtcg gtgcccggct tcttgcccgc ggcgaggaac tcgcccttgg cccagtcgcc
     7801 catcagctgc atgccggcct cgcccttgat gaccatcgcg gtcgccaggt tccagtcgcg
     7861 gcccgggctg ttcttgtcga tgtagccgtg gaccttcttg aaggtgtcga gcaccttgat
     7921 catcgtcggg ccgttgatcg tcgccggatc gagctgcacc agggccttgc ggtagaagtc
     7981 ggggccgccg acgccgagcg cgacatcctc gaacaccgtg aagtcctgcc agttctggcc
     8041 accgtgggcc agcgggatga tgcccgcggc cttgagcttg tcgccggcgg cgaagaactc
     8101 gtcccaggtc gtcgggacct tggcgccggc cttcttgaac gcttccgggt tggcccacag
     8161 ccagttcacg cggtgcacgt tgaccggcgc ggcgatgtag tggcccttgt acttcatgac
     8221 gttggcgacg accggcggca ggacggagtc ccacttctcg gccttggcga cgtcgtcgat
     8281 gttggcgagc acgccttcgg cggcccattc ctgcagggcc gggcccttga tctgcgcggc
     8341 cgcgggggcg ttgccggaga tgacgcgcga cttcagcacc gtcatggcgg agtcgccgcc
     8401 gccgccggcg accgcgaagt ccttccaggt gtggccttcg gcttccatct ccttcttcag
     8461 ctcggcggcg gccttggctt cgccgcccga ggtccaccag tgcaggactt cgagttcatc
     8521 ggcatgcgcg acgccggcac cgaggatggc ggccagcgcg acgacggtca ggcgcggaaa
     8581 cgacttggac atggatttgt ctccaaacta attcgtgatt gcaacgggag ccgcgaccga
     8641 gggtgcggtc gcggcagggc tggctacgcg gtcagaacca gatctcgacc tgggcgccgt
     8701 aggacacggc gttggtcttg tccggacgga agccgtagtt gttgtaggtg tcctgcgcgg
     8761 cggccttgtt gtagtcggcg taggtcacgt acaggcgcag ctcggggcgc ttccagaagt
     8821 ccgggccggt agacagcgcc gggccgacgg taaccttggt gacgttctcg ctggggccgc
     8881 ccgagggctt caggctggag tagccgatct ccgtgagcca cttgacgttc ttggtcagcg
     8941 cgtacgagcc gcggccgccg atcgtccaga agttggacga cggcgaggtc gac
//
LOCUS       DASVLN010000961         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_265934, whole genome shotgun sequence.
ACCESSION   DASVLN010000961 DASVLN010000000
VERSION     DASVLN010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265934"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..959
                     /locus_tag="VF453_22585"
     CDS             45..959
                     /locus_tag="VF453_22585"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HEX7690508.1"
                     /translation="MNIQDILDRIHDELRPVIGQGRVASYIPELAKVSPHRFGMAVVG
                     IDGRVTTVGDAHVPFSIQSISKLFTLVMALQLEGEGVWERCGREPSGTPFNSLVQLEA
                     EAGKPRNPFINPGALVCTDILCTRYAVPENAVVEFLRPLCGNREMHYDRAVAASERRT
                     AHRNAAMAHLMKSFGNLRNDVDDVLDAYCRHCAITMSCVELARAVGFLAHGGRHPGDG
                     REVLGPSLTKRLNALMLTCGTYDAAGDFAFRVGLPAKSGVGGGIVAVMPGECGVCVWS
                     PALEPSGNSLAGSLALERFTDLTGRSIF"
     gene            complement(967..2232)
                     /locus_tag="VF453_22590"
     CDS             complement(967..2232)
                     /locus_tag="VF453_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HEX7690509.1"
                     /translation="MTDLLDPLLDATFKGFPHDAPPLRRSRVGAQGWNVLAGDLPLPL
                     AVLKRDALVHNLGWMQRFADDAGLALAPHGKTTMSPQLFRRQLDAGAWGLTFATVAQL
                     RVGIAAGARNLILANQVVDAGDLRAIAALREAHGARIVFLLDSEAQLALIEELRPTVP
                     FEVLLEVGVPGGRTGCRTHEEALALARRARISADVALVGLECFEGLGGTGDEAQDAPP
                     ATALMDRVARLARACDEERLFEAPSVILSAGGSGLFDLVVPALRTALSRPVEGLLRSG
                     CYVTHDQGHYRRLVGAVNRRLGCDDRRGLRAALEVWATVQSMPEAGLALLAAGKRDLS
                     FDLGLPVPVALSVRGSREVLPAPAGWAISRLNDQHAYLQLNGDAAGLRVGDRVALGIS
                     HPCTTFDKWRWMPVVDERYGVVDAIVTAF"
BASE COUNT          348 a          952 c          929 g          308 t
ORIGIN      
        1 ccgccgcacg cgccgacgcc cgtcccacgc atactgacgc acgcatgaac atccaggaca
       61 tcctcgaccg catccacgac gagctgcgcc ccgtgatcgg ccaaggccgc gtcgcgagct
      121 acatccccga gctggccaag gtgtcgccgc accgcttcgg catggcggtg gtcggcatcg
      181 acggccgcgt cacgacggtc ggcgacgcgc acgtgccgtt ctcgatccag tcgatctcca
      241 agctgttcac gctcgtgatg gcgctgcagc tcgagggcga aggcgtctgg gagcgctgcg
      301 gccgcgagcc ctcgggcacg ccgttcaact cgctggtgca gctggaggcc gaggcaggca
      361 agccgcgcaa cccgttcatc aacccgggcg cgctggtgtg caccgacatc ctgtgcacgc
      421 gctacgccgt gcccgagaac gcggtcgtcg agttcctgcg cccgctgtgc ggcaaccgcg
      481 agatgcacta cgaccgggcg gtcgcggcgt cggagcgccg cacggcgcac cgcaacgccg
      541 cgatggcgca cctgatgaag tccttcggca acctgcgcaa cgacgtcgac gacgtgctcg
      601 acgcctactg ccgccactgc gcgatcacca tgagctgcgt cgagctcgcg cgcgcggtcg
      661 gcttcctcgc gcacggcggc cgccatcccg gcgacggccg cgaggtgctc ggccccagcc
      721 tgaccaagcg cctgaacgcc ctgatgctga cctgcggcac ctacgacgcg gcgggcgact
      781 tcgccttccg cgtcggcctg ccggccaaga gcggcgtcgg cggcggcatc gtcgcggtga
      841 tgccgggcga gtgcggagtc tgcgtctggt cgccggcgct cgaaccctcg ggcaactccc
      901 tggccggctc gctggcgctg gagcgcttca ccgacctgac ggggcggtcg atcttctgag
      961 tcgccgtcag aacgcggtga cgatcgcgtc gacgacgccg tagcgctcgt cgacgaccgg
     1021 catccagcgc cacttgtcga aggtcgtgca cgggtgcgag atgcccagcg cgacgcggtc
     1081 gccgacgcgc aggccggcgg cgtcgccgtt gagctggagg taggcgtgct ggtcgttgag
     1141 ccgggagatc gcccagccgg ccggcgcggg cagcacctcg cggctgccgc gcacgctcag
     1201 cgcgacgggc acgggcagcc cgaggtcgaa cgacaggtcg cgcttgccgg cggccagcag
     1261 cgcgaggccg gcctcgggca tcgactgcac ggtcgcccac acctcgagcg ccgcgcgcag
     1321 gccacggcgg tcgtcgcagc ccaggcgccg gttgacggcg ccgacgagcc gccggtagtg
     1381 cccctggtcg tgcgtgacgt agcagcccga gcgcagcagg ccctcgaccg gccgcgacag
     1441 cgccgtgcgc agggccggga cgacgaggtc gaacagcccc gagccgccgg ccgacaggat
     1501 cacggacggc gcctcgaaca gccgctcctc gtcgcaggcg cgcgcgaggc gggcgacgcg
     1561 atccatcagc gcggtcgcgg gcggcgcgtc ctgcgcctcg tcgccggtgc cgccgaggcc
     1621 ttcgaagcac tcgaggccga ccagcgcgac gtccgcgctg atgcgggccc ggcgcgcgag
     1681 cgcgagcgcc tcctcgtgcg tgcggcagcc ggtgcggccg ccggggacgc cgacctcgag
     1741 cagcacctcg aagggcacgg tcgggcgcag ctcctcgatc agcgcgagct gcgcctcgga
     1801 atcgagcagg aacacgatgc gcgcaccgtg cgcctcgcgc agcgcggcga tggcccgcag
     1861 gtcgccggca tccacgacct ggttggcgag gatcaggttg cgcgcgcccg cggcgatgcc
     1921 cacgcgcagc tgcgcgaccg tcgcgaaggt cagcccccac gcgccggcgt cgagctggcg
     1981 ccggaacagc tgcggcgaca tcgtcgtctt gccgtgcggc gcgagcgcga ggccggcgtc
     2041 gtcggcgaag cgctgcatcc agccgaggtt gtgcacgagg gcgtcgcgct tgagcacggc
     2101 cagcggcagc ggcaggtcgc ccgcgagcac gttccagccc tgcgcgccga cacgcgagcg
     2161 ccgcagcggc ggcgcgtcgt gcggaaagcc cttgaacgtc gcgtcgagca gggggtcgag
     2221 cagatcggtc atccgaccat cataggcgcg ccggcgcgag tgccaacttc gtcacggaat
     2281 cgccgcggcc gagcttggcg gcggcaagga cttcacgccg gatgcgcgcg cgtcgccgcg
     2341 gcgcctgaag tccgcgcgcg cgcaggccga taacgggccg atgcgccccg ccgactcctt
     2401 ccgtcttccg tcccgccgac ggccccggct cccaggcctg ctcgcgggga cgctggcgct
     2461 cgccctggcc ccggcgctgg cggccaccgg caagctgcgg ctgaccggcg gcgtgacgag
     2521 cgtcgacggc gcggccg
//
LOCUS       DASVLN010000962         3667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_266072, whole genome shotgun sequence.
ACCESSION   DASVLN010000962 DASVLN010000000
VERSION     DASVLN010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266072"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1379
                     /locus_tag="VF453_22595"
     CDS             276..1379
                     /locus_tag="VF453_22595"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7690510.1"
                     /translation="MGLDKKIATTLAVAAAVACGAAHASEDYDLRYAPGIGGADMSAP
                     FEAGWVFQAPAYVYSGHVSSQLHQPVDLSSSVPGASALATIDLRTQINVQGLLPRLSF
                     MSDTKFLGAQVGGTALLPVVTKRASTRVTGVSSAVNAPGMPASVISQLEAGTNAAVTQ
                     GANVSAADNSNEATGIGDLELSPILRWSTDASQTLFVLTVVAPTGDFRADRAANPGAG
                     RFWTLRPAVQYSYIGDGWDFGGRLAYSINTRDHATQYRTGNYLNVDATLMKSLNDSTR
                     IGLSGYAVAQTTRDTSQFAGPASATDQAGIDEAARQGVTVDQKGHVYGLGPEVAYIHG
                     AGDYLLEGRFMKEFAGSYRPQGFTAIVTLSKPF"
     gene            1416..2096
                     /locus_tag="VF453_22600"
     CDS             1416..2096
                     /locus_tag="VF453_22600"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX7690511.1"
                     /translation="MTMQLLRRAAAALALGLAVSGAWAAVEVSGVPLDETVTVGGTPL
                     TLNGAGYRKRGYFKVDVTALYTQQRVNSLEALENAKGPRRVELVIQQDITGSQASRYF
                     LIDFEASATPAEFAQLISEVSQIGDIYSSLPKIKKGDIVAIDWLPGKGLTAMLNGNLL
                     TPHGASSPYLNSELLGKVMFRMYVGGKTPAELRDNLLGLSTSMRDKGSSTAAAAPGPA
                     ATGNTAKH"
     gene            2117..2965
                     /locus_tag="VF453_22605"
     CDS             2117..2965
                     /locus_tag="VF453_22605"
                     /inference="COORDINATES: protein motif:HMM:NF022461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2846 domain-containing protein"
                     /protein_id="HEX7690512.1"
                     /translation="MNRRVLLATTLLAAAWLAGCASGPKFSEVSGSIGPIPAGDGRIF
                     FFRSSSMMGAAIQPDIRLNGEVVGTSKPGGFFFVDRPAGGYTAATSTEVEKATTFSLA
                     PGETKYLRTSPSFGLLVGHIAVDVEDPRKAQAEIETLSMTGGAPATPGRSAAPDAAAP
                     AAKPAPVAEPPLDASGRPAHLRPLDAAALEGRTWIFPHPRDPARLQDVRIRFAGGVAS
                     ASNARSASTGPYTVKDDMVCIDFQSRDWGHTCYYVYEAAPAAGGSPAPMLWTVYTHRA
                     VPLTIE"
     gene            complement(2990..3388)
                     /gene="gloA"
                     /locus_tag="VF453_22610"
     CDS             complement(2990..3388)
                     /gene="gloA"
                     /locus_tag="VF453_22610"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX7690513.1"
                     /translation="MTLLHTMLRVGDLQRSIDFYVRHFGMTLRRTTDRPEQQYSLAFL
                     GFGPGGAHGEGGAEIELTYNYGVDKYELGTAFGHLAFGVPDVAAACERVRAGGGTVTR
                     EAGPVKGGTTVIAFVQDPDGYKIELIQLGT"
     gene            complement(3385..>3667)
                     /locus_tag="VF453_22615"
     CDS             complement(3385..>3667)
                     /locus_tag="VF453_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HEX7690514.1"
                     /translation="PAGYVRASAGGPHAPEAYGAPPAVAWEALMPRIADFWGRVADRV
                     EPRHRAGRLKQWLNLLRRRYPEAQRAYDELRLVNDPARLAAAMFGTVSA"
BASE COUNT          570 a         1348 c         1234 g          515 t
ORIGIN      
        1 cgcgtctgaa cgccgtcgcc tcggcgacag gcgatgcccc ggaaagacac gagagtaggt
       61 cgagctaccc gcttccgtcg cgttttccgc acgatcgttc gcatgctgtc aaggaatttt
      121 cgaacgaccg tgcatttgtc atcaacacgc gacatttccg tcggtgcgaa tccctagacg
      181 cgcctttatg tgcgtaattt ccatgttgcc gccgtcgagc gccgaataga ctggcgcccg
      241 tcttgaaacc attaccagac ccggagacaa cccgcatggg cctcgacaag aagatcgcca
      301 ccacccttgc cgtcgcagcc gccgtcgcct gcggcgccgc gcacgcgtcg gaagactacg
      361 acctgcgcta tgcgccaggc atcggcgggg ccgacatgtc cgccccgttc gaggccggct
      421 gggtgttcca ggcgccggca tacgtctaca gcggccacgt cagctcgcag ctgcaccagc
      481 cggtggacct gtcgtccagc gtccccggcg cgagcgccct ggcgacgatc gacctgcgca
      541 cccagatcaa cgtccagggc ctgctgccgc gcctgagctt catgagcgac acgaagttcc
      601 tcggcgccca ggtcggcggc acggcgctgc tgcccgtcgt cacgaagcgc gcgagcaccc
      661 gcgtcaccgg cgtgtcctcg gccgtgaacg ccccgggcat gccggcctcg gtgatctcgc
      721 agctggaggc gggcaccaac gccgccgtca cccagggcgc caatgtctcc gcggccgaca
      781 actccaacga ggccacgggc atcggcgacc tcgagctgtc gcccatcctg cgctggtcga
      841 ccgacgcgag ccagacgctg ttcgtgctga ccgtggtcgc gcccaccggc gacttccgcg
      901 ccgaccgcgc cgccaacccc ggcgccggcc gcttctggac gctgcgcccc gcggtgcagt
      961 acagctacat cggcgacggc tgggatttcg gcggccggct cgcgtattcg atcaacaccc
     1021 gcgaccatgc gacccagtac cgcacgggca actacctcaa cgtcgacgcg acgctgatga
     1081 agtcgctcaa cgactcgacg cgcatcggcc tgtcgggcta cgccgtcgcc cagacgaccc
     1141 gcgacacgag ccagttcgcg ggccccgcct ccgcgacgga ccaggccggc atcgacgagg
     1201 ccgcgcgcca gggcgtgacc gtcgaccaga agggccacgt ctacggcctc ggccccgagg
     1261 tcgcctacat ccacggcgcg ggcgactacc tgctcgaagg ccgattcatg aaggagttcg
     1321 cgggctccta ccgtccgcag ggcttcacgg ccatcgtcac gctgtccaag ccgttctgaa
     1381 cgcgggcgcc ccgcgcctgg aaggagggtg aaaccatgac catgcaactc ctgcgccgcg
     1441 cggccgccgc gctcgcgctc ggcctggccg tctccggcgc gtgggccgcc gtggaggtct
     1501 cgggcgttcc gctggacgag accgtcaccg tcggcggcac cccgctgacg ctcaacggcg
     1561 ccggctaccg caagcgcggc tacttcaagg tcgacgtcac ggcgctgtac acgcagcagc
     1621 gcgtcaactc gctggaggcg ctcgagaacg ccaagggccc gcgccgcgtc gagctggtga
     1681 tccagcagga catcaccggc tcgcaggcct cgcgctactt cctcatcgac ttcgaggcct
     1741 ccgccacgcc ggccgagttc gcgcagctga tcagcgaggt ctcgcagatc ggcgacatct
     1801 acagcagcct gccgaagatc aagaagggcg acatcgtcgc gatcgactgg ttgccgggca
     1861 agggcctgac cgccatgctc aacggcaacc tgctgacccc gcacggcgcc tcgtcgcctt
     1921 acctgaacag cgagctgctg ggcaaggtca tgttccgcat gtacgtcggc ggcaagacgc
     1981 ccgcggagct gcgcgacaac ctgctggggc tgtcgacgtc gatgcgggac aagggatcgt
     2041 cgacggcggc ggccgcgccg ggccccgctg ccaccggcaa taccgcaaag cactgacgcc
     2101 ccgggaggac aggcccatga atcgacgcgt cctgcttgcc accaccctgc tggccgcagc
     2161 ctggcttgcc ggctgcgcga gtggaccgaa gttttccgag gtctcgggct cgatcgggcc
     2221 catccccgcg ggcgacggac ggatcttctt cttccgctcg agctccatga tgggcgcggc
     2281 gatccagccg gacatccgcc tcaacggcga ggtggtcggc acctccaagc cgggcggctt
     2341 cttcttcgtc gaccggcccg ccggcggcta cacggcggcc acgtcgaccg aggtggagaa
     2401 ggccacgacg ttcagcctcg ctcccggcga gacgaaatac ctgcgaacct cgcccagctt
     2461 cgggttgctg gtggggcaca ttgccgtcga cgtggaggat ccccggaagg cgcaggcgga
     2521 aatcgagacc ctcagcatga cgggcggggc cccggcgacg ccaggccgca gcgcggcacc
     2581 ggacgccgcg gcgcctgcgg cgaagcccgc gcccgtcgcg gagcccccgc tggacgcaag
     2641 cggccgtccc gcccacctgc gcccgctcga tgccgccgcg ctggaaggac gcacgtggat
     2701 cttcccccac ccgcgtgatc cggcacgcct gcaggacgtc cggatccgct ttgccggcgg
     2761 cgtggccagc gcctccaacg ccaggagtgc gagcacgggc ccgtacaccg tcaaggacga
     2821 catggtctgc atcgacttcc agtcgcgcga ctggggccat acctgctact acgtctacga
     2881 ggccgcgccc gcggccggcg gctcgccggc gcccatgctg tggacggtgt acacgcacag
     2941 ggcggtgccg ctcacgatcg aatgaggcgc cggcgccggg ccgggtcgct caggtgccca
     3001 gctggatcag ctcgatcttg tagccgtccg gatcctgcac gaacgcgatc accgtggtgc
     3061 cgcccttgac cggaccggcc tcgcgcgtga cggtgccgcc gccggcgcgc acgcgctcgc
     3121 aggcggccgc gacgtcgggc acgccgaagg caaggtggcc gaaggcggtg ccgagctcgt
     3181 acttgtcgac gccgtagttg taggtgagct cgatctcggc gccgccctcg ccgtgcgcgc
     3241 cgcccggccc gaagccgagg aacgcgagcg agtactgctg ctcgggccgg tcggtcgtgc
     3301 ggcgcagcgt catgccgaag tggcggacgt agaaatcgat ggagcgctgc aggtcgccga
     3361 cgcgcagcat ggtgtgcagg agtgtcatgc cgagacggta ccaaacatcg cggccgcgag
     3421 cctggccggg tcattgacca gccgcagctc gtcgtacgcg cgctgggcct cgggatagcg
     3481 ccgacgcagc aggttgagcc attgcttgag gcggccggcg cggtggcgcg gctcgacgcg
     3541 gtcggcgacg cgcccccaga agtcggcgat gcgcggcatc agcgcctccc aggcgacggc
     3601 cggcggcgcg ccgtaggctt cgggggcgtg cgggccgccg gcgctcgccc gcacatagcc
     3661 cgcggga
//
LOCUS       DASVLN010000963         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_373323, whole genome shotgun sequence.
ACCESSION   DASVLN010000963 DASVLN010000000
VERSION     DASVLN010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373323"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1024)
                     /locus_tag="VF453_22620"
     CDS             complement(80..1024)
                     /locus_tag="VF453_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690515.1"
                     /translation="MAHRILSSTRLALATGLLAAALAAPGHAAGVGADAAAGKSTPDA
                     ASADLRHGYSYRTLDYPGASQTIFWGLDDFGRLAGQYTLPGGVAHAMTWGHGQFEALA
                     PERLGTWFSAAGGPTDTGTTFGGYADASGLQHGFVIRRGHFETIDFPGHRNSNVDAVD
                     LAGTILGVYWDADGVYHGILSCNGNDTPIDVPGARDTYPLGMNDRGDSVGYWDAGGAA
                     THGYVRRADGQLATMDVPEASSTVAFAINGRGQVAGYFKDATGALHGFVKTGAHVRQL
                     DMPGAVATIVTALNNWGSVAGEYFDAGGVRHGFVAMPE"
     gene            1185..1727
                     /locus_tag="VF453_22625"
     CDS             1185..1727
                     /locus_tag="VF453_22625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX7690516.1"
                     /translation="MNADPTITDILARASAGDRAAIDGLIPRIYATLRRLAHRQRMGE
                     QAHTLETTALVHEACLQLLNRGDAVFPDRAHLYAYMSQAMRHILVDHARRHRAQKRQA
                     PELAEEPPRSDILDVLAIDEAMNRLAAVDARLARVAELRLFADLSSPEISQVLGVNAR
                     TVERDWLKARTFLSACLTPG"
     gene            1753..>2732
                     /locus_tag="VF453_22630"
     CDS             1753..>2732
                     /locus_tag="VF453_22630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7690517.1"
                     /translation="MALTQEEDASPMTIAIAGISERWPEIEPLIDRLFDVPADERAEW
                     LRTHCGDAGVRAFVAQALDDAPGVQSLERGMAQWLPALVDELDATLPAIPGYRVLRFV
                     GAGGMASVFEAQRELPGGPQTVALKLLRIDVHDADERRGFLREQRILARLQHPHIAQL
                     LDAGFTPTGTPFLALEFVAGDDLVAHCERHALGTRERLALFMDVCAAVEHAHRNLIVH
                     RDLKPSNVLVGAEGRVKLVDFGIAKLLTGDAEPTRTQARRLTRAYAAPEQFAGEATTT
                     SIDVYALGVLLAELLGGRRPHRAGGALDAHGPAIDHEALRRGLGVDLHAIV"
BASE COUNT          368 a          993 c         1003 g          368 t
ORIGIN      
        1 gcctccccgg tcgggatgat atgcggcggc cgccggcccg cgccgtcgcc gccgtcgccg
       61 caggccggcg cccgtgcgct cactccggca tggccacgaa cccgtggcgc acgccgccgg
      121 cgtcgaagta ctcgccggcg acgctgcccc agttgttgag cgcggtgacg atcgtcgcca
      181 cggcgccggg catgtcgagc tgccggacat gcgcgcccgt cttgacgaag ccgtggagcg
      241 cgccggtcgc gtccttgaag tacccggcca cctggccgcg gccgttgatc gcgaaggcga
      301 ccgtgctcga cgcctcgggc acgtccatcg tggcgagctg gccgtccgcg cggcggacgt
      361 agccatgcgt cgccgcgccg cccgcgtccc agtagccgac cgagtcgccc ctgtcgttca
      421 tgccgagcgg gtaggtgtcg cgcgcgccgg gcacgtcgat cggcgtgtcg ttgccgttgc
      481 agctcaggat gccgtggtag accccgtccg cgtcccagta cacgccgagg atcgtgccgg
      541 cgaggtcgac cgcgtccacg ttggaattgc ggtggcccgg gaagtcgatc gtctcgaagt
      601 ggccgcggcg gatgacgaat ccgtgttgca ggcccgaggc gtcggcatag ccgccgaagg
      661 tcgtgccggt gtcggtcggg ccgccggcgg cgctgaacca ggtgcccagc cgctcgggag
      721 cgagcgcctc gaactggccg tgcccccagg tcatcgcgtg cgcgacgccg cccgggagcg
      781 tgtactggcc ggccagcctg ccgaagtcgt cgaggcccca gaagatggtc tgcgatgcgc
      841 cggggtagtc gagcgtgcgg tacgagtagc cgtgccgcag gtcggcggaa gcggcgtctg
      901 gtgtactctt gcccgcggcc gcgtcggccc cgacgccggc ggcatggcca ggtgcggcga
      961 gggcggcggc gagcaggccg gtcgccaggg cgagccgggt ggacgaaagg atgcgatggg
     1021 ccatggaggc actccgtgaa gtggatgggg agcgacggca tgccggcgac cgatcgcggt
     1081 cccgtggcgc gccatccctt ccttaagcgc aaagcggtcg cccgcaccga catcgcagga
     1141 acacgaacga cgtttcgcgg cgccgcgccg tcgcgcccca gtgcatgaat gccgatccga
     1201 cgatcaccga catcctcgcg cgcgcgtccg cgggcgaccg cgccgcgatc gacggcctga
     1261 tcccgaggat ctacgcgacg ctgcgccgcc tcgcccaccg ccagcgcatg ggcgagcagg
     1321 cccacacgct cgagacgacc gcgctcgtcc acgaggcctg cctgcagctg ctgaaccggg
     1381 gcgatgccgt cttccccgat cgcgcgcacc tgtacgcata catgagccag gcgatgcgcc
     1441 acatcctcgt cgaccacgcc cgtcgccacc gcgcgcagaa gcgccaggcg ccggagctgg
     1501 ccgaggagcc gccgcgctcg gacatcctgg acgtgctggc gatcgacgag gccatgaacc
     1561 ggctggccgc ggtcgacgcg cggctggccc gcgtcgccga gctgcgcctg ttcgccgacc
     1621 tgtccagccc ggagatctcg caggtgctcg gcgtcaacgc gcgtaccgtc gagcgcgact
     1681 ggctgaaggc gcgcacgttc ctgtccgcct gcctgacgcc gggctgaagg ccgtgtcggc
     1741 gatcgaggcg cgatggcgct gacgcaggag gaggacgcgt cgccgatgac gatcgccatt
     1801 gccggaattt ccgaacgctg gccggagatc gagcccctga tcgatcgcct gttcgacgtc
     1861 cccgccgacg agcgcgccga gtggctgcgc acgcactgcg gggatgccgg cgtgcgcgcg
     1921 ttcgtcgcgc aggcgctgga cgacgccccc ggggtgcagt cgctcgagcg cggcatggcg
     1981 cagtggctgc cggcgctcgt cgacgagctc gacgcgacgc tgcccgccat ccccggctat
     2041 cgcgtgctgc gcttcgtcgg cgcgggcggc atggccagcg tgttcgaggc acagcgcgag
     2101 ctgcccggcg gcccgcagac ggtcgccctg aagctgctgc gaatcgacgt ccacgacgct
     2161 gacgagcgcc gcggcttcct gcgcgagcag cggatcctcg cgcggctgca gcatccgcac
     2221 atcgcgcaac tgctcgacgc ggggttcacg ccgacgggca cgccattcct ggcgctcgag
     2281 ttcgtcgccg gcgacgatct cgtcgcgcac tgcgagcgcc acgccctggg cacgcgcgag
     2341 cggctcgccc tgttcatgga cgtgtgcgcg gcggtggagc atgcgcaccg caacctgatc
     2401 gtgcaccgcg acctgaagcc gagcaacgtg ctggtcggcg ccgaagggcg cgtcaagctg
     2461 gtggacttcg gcatcgccaa gctcctgacg ggcgacgccg agccgacccg cacgcaggcc
     2521 cggcgcctca cgcgcgcgta cgcggcgccc gagcagttcg cgggcgaggc gacgacgacg
     2581 tccatcgacg tctacgcact cggcgtgctg ctggcggagt tgctgggcgg gcggcggccg
     2641 catcgcgccg gcggcgccct cgacgcgcac ggaccggcga tcgaccacga ggcgctgcgg
     2701 cgcgggctcg gcgtcgatct ccacgcgatc gt
//
LOCUS       DASVLN010000964        13030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_105316, whole genome shotgun sequence.
ACCESSION   DASVLN010000964 DASVLN010000000
VERSION     DASVLN010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 13030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13030
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105316"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VF453_22635"
     CDS             <1..1080
                     /locus_tag="VF453_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7690518.1"
                     /translation="LAATHTRETGAPTVDAFGLGVSAWELDLWGRVRSLGDAALAQLA
                     ASEQARAAAQVSLVASVASAYVAMAGDQALMDLAQRTYDTRTETMKLVQLRFEHGVAS
                     SIELQDNRSLVEAARVALAQAKRQRALDEDALVLLVGSPLPADLPPAPTWESIALPDV
                     PVGLPSQVLLQRPDVAQAEDQLRAANADIGAARAAFFPTISLTGSAGTASTSLDHLFE
                     HAAFTFIPQVTLPIFDAGRNRANLRISQAQRDIAVAQYEKAVQSAFRDVADALAGRAT
                     LVEQLDAQQAQADAEAKRFELSDLLYKNGVASSLDQLDAQRSVFAAQQALVQTRLLLL
                     QNRIAVYRAIGGGWSDEDAQAAASR"
     gene            complement(1094..1834)
                     /locus_tag="VF453_22640"
     CDS             complement(1094..1834)
                     /locus_tag="VF453_22640"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX7690519.1"
                     /translation="MTDPRTATSAGGVVGVLGGMGPLATVDFLSKMLAATPAANDQEH
                     VPFVVSAIPQVPDRTLAFRGEGESPLPAMVDSARRLKAAGAGLVVIVCNTAHLWFDEL
                     REAVGLPMIHLVDAAIDEAVELAGADGRVGLLGTDATVASGLYFNRGTSAAAPIQWRL
                     PTAREMTEFVMPGIAAVKAGELEAGRARLAAATEALKGRGATVVILGCTEIPVVLDAG
                     SSPLPVVDATAALARRAVSWSRARRAGA"
     gene            complement(1867..3141)
                     /locus_tag="VF453_22645"
     CDS             complement(1867..3141)
                     /locus_tag="VF453_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX7690520.1"
                     /translation="MKNRLTLYISLGMLLGVLAGWLGHQAADPKAVADRYAILTDIFL
                     RLIKMIIAPLVISTLVSGLAGMGSGRDIGRIGVRSIAWFACASLVSLALGLALANLFQ
                     PGAGLHMTAGGAAVDTGLNTSGLNVRDVVTHAIPTSLLDAMARNDIIQIVVFSLFFGL
                     GLNVLKGNPAVDAVIGAVDGLVPVMLKLTGYVMRFAPLGVFGALAAAVTLKGLDVLVT
                     YGRLLSTFYVGLAALWAVLVAVGYGFLGKRIGALLREVREPALIAFSTASSEAAFPRL
                     AEKLERFGIDKKVVGFTLPLGYAFNLDGSMMYQSFAAIFIAQAFGIDMPLSQQVVMLL
                     VLMISSKGMASVPRGSVVVVAAIAPMFRLPMEGVALVLAIDQLLDMGRTMTNVIGNSI
                     ATAVVAHLEQRRGAAAPAAQALPAAVPQATGR"
     gene            complement(3306..4226)
                     /locus_tag="VF453_22650"
     CDS             complement(3306..4226)
                     /locus_tag="VF453_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7690521.1"
                     /translation="MEIKWIEDFVALAQYQSFSRAAEERNVSQSGFSRRIQALEQWVG
                     ADLIDRSTYPPSLTAAGRLFKEAAEEVLLKLYDARAVIREDRRLPGPGLQIAAGHTIA
                     LNFLSQWLVELPAQFHEVRTRVVPTNVHDSIVMLANANCDLMFAYHHPSLPLHLDPSK
                     YEHLSLGHDALLPVCRAADGGGPLFRLSAGAQARPAPLLGYSEGSYFGRCLEFLLRQA
                     RSAPALQRHYESDMAELLKKMALAGEGVAWLPHSAIAGELARGELIAAGGGDWRMELE
                     IRVYRDATQRNPLVATLWKALQDRRAGRTA"
     gene            4520..4984
                     /locus_tag="VF453_22655"
     CDS             4520..4984
                     /locus_tag="VF453_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7690522.1"
                     /translation="MKGKTSSPSPMEAHLGFWLRFISNHVSARFQQMLEAKGVSVTEW
                     VALRTLWARDETRHGELIQALGMTKGAASKVVSRLEEKGLAERRFASGSPRDQSMVLT
                     PAGKAIVPLLASIADANDNHFFGHLDVRERQALMAAMQSLVQHHGLKDIPTN"
     gene            5020..5415
                     /locus_tag="VF453_22660"
     CDS             5020..5415
                     /locus_tag="VF453_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HEX7690523.1"
                     /translation="MNETTRTAIHATFEASNKGQIHFGEVIAQLMAAQVESYHVDYRS
                     GRTTYYLRDGATLDYGFERPQEAIPEAFDANAVRGAILGAQQGRVMYPEFKQLSQRAG
                     CVGYTVWIAGRHVAYLGRRGETHIEHFPN"
     gene            complement(5642..6097)
                     /locus_tag="VF453_22665"
     CDS             complement(5642..6097)
                     /locus_tag="VF453_22665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690524.1"
                     /translation="MADATPFFGISPFVLGMSRETVHAAAGRPDSVETSQDEDGSVVE
                     SWFYQGGEIELEFDQAADARLESITAWSSTTTVNGVAIIGQPVEELARLAREADIHDL
                     ELTDDFADAGQCFQSEQHGLMFWVAKGRIANVTIFPRFDDSGETPQWPE"
     gene            complement(6136..6684)
                     /gene="ruvC"
                     /locus_tag="VF453_22670"
     CDS             complement(6136..6684)
                     /gene="ruvC"
                     /locus_tag="VF453_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX7690525.1"
                     /translation="MRILGIDPGLNTTGFGVIESEGAALRYVASGTISTAKMAKGDLP
                     GRIRTLFDGVREVTQRYAPEHAALEIVFVNVSPQSSLLLGQARGAALAALVSAGLPVA
                     EYTAAQMKKAVGGSGGAKKAQVQEMVARLLALPGLPGTDAADALGLAIMHAHAGRTMA
                     ALGAATTLARRQHAQYRGGRTY"
     gene            complement(6684..7658)
                     /locus_tag="VF453_22675"
     CDS             complement(6684..7658)
                     /locus_tag="VF453_22675"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7690526.1"
                     /translation="MMALKALTAQQQIGLLFLLLFGGLLLVSAGTVLVAMRERHAASR
                     RGLTAFEANLRATWIGAVIFWLSWVMGAGGATILFAIVSFLALREFVTLMQTRRADHR
                     SLMLAFFVVLPVQFVLAGSQSFDLFTVFIPVYVFLAIPVLSALGGDPLRFLERNAKIQ
                     WGVMVCVYGLSHAPALLLLQVPHAHDRGAFLVFFLVVVTTAAQILEDIASHRLRRRPV
                     ARLIDRRFGLQSWAVACFGAAVVGGLLFWITPFKLPQAMAMAFIAGAAGTLGEFVMTA
                     LKRDAGVNYWGNSGAVTGAVGLLDRVAPLCFAAPVFFHSARWFFQAHG"
     gene            complement(7655..8299)
                     /locus_tag="VF453_22680"
     CDS             complement(7655..8299)
                     /locus_tag="VF453_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7690527.1"
                     /translation="MNAADAWGFVLAAVARLITGAQGHWKGVAPKAEQRIYFANHQSH
                     LDWVLIWATLPADLRQQTRPIAAADYWTTGKIKHWLTREVFRAVYVDRERKDPSSDPL
                     APLVAALEQGDSLVIFPEGTRSNKGLPQEFKSGLYHLARKFPHVQLIPAWVDNVQRVM
                     PKGEIVPVPVLCTVTFGAPCNLAPDETKADFLARARAAVVALKPGGAAALEHAS"
     gene            complement(8373..9176)
                     /gene="lgt"
                     /locus_tag="VF453_22685"
     CDS             complement(8373..9176)
                     /gene="lgt"
                     /locus_tag="VF453_22685"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7690528.1"
                     /translation="MLLHWNFDPIALQLGPVAIHWYGICWGLAFFGAEFGVRRMLARL
                     GRHDVDVSGLIICALFGTVVGARLAHCLFYDPAFYLAHPLKILAIWEGGMASHGGALG
                     LIAALAWGAPRYAPGLPLLTLLDVAAPSAALGGAIIRCANFLNSEIVGNPTGGDWGVV
                     FERVDALPRHPVQLYEAAAYLAVLAVLLAVDRGGRALRRRGELTGLFMALVFAARIVI
                     ETWKTPQAAYEAGQAFTVGQWLSVPFVVLGVVLVLRARRSAPALAPSRR"
     gene            complement(9200..10780)
                     /gene="purH"
                     /locus_tag="VF453_22690"
     CDS             complement(9200..10780)
                     /gene="purH"
                     /locus_tag="VF453_22690"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX7690529.1"
                     /translation="MSLTALLSVSDKTGIVDFARALHAQGVKLLSTGGTAKLLAESGL
                     PVTEVSEVTGFPEMLDGRVKTLHPRVHGGLLARRDVPEHMAALKEHGIGTIDLLVINL
                     YPFAKATARPDCTLEDAIENIDIGGPAMLRAAAKNWQDVGVVIDPADYPQVLSELSAG
                     GLQRKTKFALAKKVFAHTAAYDGMITNYLTALAADAENDGAAVPARGDWPATFNLQLT
                     KVQDMRYGENPHQGAAFYRDASPAPGTLAAWTQLQGKELSYNNIADADAAWECVKTFD
                     VPACVIVKHANPCGVAVGADAHEAYSKAFKTDPTSAFGGIIAFNRPLDRAAAEAVVRQ
                     FVEVVIAPAIRPEAHAVFAPKQNVRLLEVPLPAERVMNAIDFKRVGGGVLLQTSDARN
                     VQPSELRIVTKVQPTPAQMDDLMFAWKVAKFVKSNAIVFCAGGMTLGVGAGQMSRVDS
                     ARIAGIKATNANLSLQGSAVASDAFFPFRDGLDVVCDAGATCVIQPGGSMRDEEVIAA
                     ADERGIAMVLTGTRHFRH"
     gene            complement(10845..11084)
                     /locus_tag="VF453_22695"
     CDS             complement(10845..11084)
                     /locus_tag="VF453_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7690530.1"
                     /translation="MSKKQIEACVRETLEQYFKDLKGAEPHSMHEMFLATVEKPLLDV
                     VLRHADGNQSKAAEWLGINRNTLRRKLLEHRLVDQ"
     gene            complement(11103..12131)
                     /gene="dusB"
                     /locus_tag="VF453_22700"
     CDS             complement(11103..12131)
                     /gene="dusB"
                     /locus_tag="VF453_22700"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX7690531.1"
                     /translation="MHIGAIELPNALFVAPMAGVTDRPFRMLCKRLGAGYAVSEMVTS
                     RKDLWNSLKTSRRANHDGETAPISVQIAGTEPQMMAEAAAYNIDRGAQIIDINMGCPA
                     KKVCNKWAGSALMQDEPLAIGIVEAVVAACAPRGVPVTLKMRTGWCDGERNAVRLARA
                     AESAGVAMVTVHGRTREQGYKGFAEYDTVAAVKAALRIPVVANGDIDSPRKAAAVLAH
                     TRADAIMVGRAAQGRPWIFREIAHYLATGQLLPPPATRDVARWLTEHLHDHYGLYGED
                     LGVRTARKHIGWAVHALPGGPEFRAVMNTHADCATQLAGVTDYFDRLADRHVLWPAAN
                     EGLMQQEA"
     gene            12320..12799
                     /locus_tag="VF453_22705"
     CDS             12320..12799
                     /locus_tag="VF453_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX7690532.1"
                     /translation="MDDWFSPVLHWLALPHNGLPALFVVALVSATLLPLGSEPALVGL
                     LQLDPGLFWWAILVATAGNTAGGAITWATGRYAHVAWSRVAHRAPLEAKHPRLAGWLQ
                     RWGPAACVLGWLPVVGDPLCAVAGWLRMPFWPCVAFMAIGKGARYVAVAGLARAALH"
BASE COUNT         1911 a         4525 c         4757 g         1837 t
ORIGIN      
        1 ctcgcggcca cgcacacgcg cgagacgggc gcgccgaccg tcgacgcctt cggcctgggc
       61 gtgtcggcct gggagctcga cctgtggggc cgcgtgcgct cgctgggcga cgccgcgctg
      121 gcgcaactcg ccgcgagcga gcaggcgcgc gcggcggcgc aggtctcgct ggtcgcgtcg
      181 gtggcgagcg cgtatgtcgc gatggccggc gaccaggcgc tgatggacct ggcgcagcga
      241 acctatgaca cgcgcaccga gacgatgaag ctggtgcagc tgcgcttcga gcacggcgtc
      301 gcctccagca tcgagctgca ggacaaccgc tcgctggtcg aggccgcgcg cgtcgcgctc
      361 gcgcaggcga agcgccagcg cgcgctcgac gaggacgcgc tggtgctgct ggtcggcagc
      421 ccgctgccgg ccgacctgcc gcccgcgccg acgtgggaat cgatcgcgct gccggacgtg
      481 ccggtgggcc tgccctcgca ggtgctgctg cagcgcccgg acgtcgcgca ggccgaggac
      541 cagctgcgcg ccgccaacgc cgacatcggc gccgcccgcg ccgcgttctt cccgacgatc
      601 tcgctgaccg gcagcgcggg cacggcgagc acctcgctcg accacctgtt cgagcacgcc
      661 gcgttcacct tcatcccgca ggtgacgctg ccgatcttcg acgccggccg caaccgcgcc
      721 aacctgcgca tctcgcaggc gcagcgcgac atcgcggtgg cgcagtacga gaaagccgtg
      781 cagtcggctt tccgcgacgt cgccgacgcg ctcgcgggcc gcgcgacgct cgtcgagcag
      841 ctcgacgcgc agcaggccca ggccgacgcc gaggccaagc gcttcgagct gtcggacctg
      901 ctgtacaaga acggcgtcgc gagctcgctc gaccagctcg acgcccagcg ctcggtgttc
      961 gcggcgcagc aggcgctcgt gcagacccgg ctgctgctgc tgcagaaccg gatcgccgtc
     1021 tatcgcgcga tcgggggcgg gtggtcggac gaggacgcgc aggcggccgc gtcgcgctga
     1081 cgcggctcgc cggtcaggcg cctgcgcgcc gcgcccgcga ccaggacacc gcgcgtcgcg
     1141 cgagcgccgc ggtggcgtcg acgacgggca gcggcgagct gccggcatcg agcacgacgg
     1201 ggatctccgt gcagccgagg atcaccacgg tcgcgccgcg tcccttgagc gcctcggtcg
     1261 ccgcggccag gcgcgctcgg ccggcctcga gctcgccggc cttcaccgcc gcgatgcccg
     1321 gcatgacgaa ctcggtcatc tcgcgcgcgg tgggcagccg ccactggatc ggcgccgccg
     1381 ccgacgtgcc gcgattgaag tacaggcccg aggcgaccgt cgcatccgtg cccagcaggc
     1441 cgacgcgccc gtccgcaccc gcgagctcga cggcctcgtc gatggcggca tcgacgagat
     1501 ggatcatcgg cagcccgacg gcctcgcgca gctcgtcgaa ccacaggtgc gcggtgttgc
     1561 agacgatgac caccaggccg gcgcccgcgg ccttcaggcg gcgggcgctg tcgaccatgg
     1621 ctggcagcgg cgactcgccc tcgccgcgga acgccagcgt gcggtcgggc acctgcggga
     1681 tcgcgctgac gacgaagggc acgtgctcct ggtcgttcgc ggcgggcgtg gcggcgagca
     1741 tcttggacag gaagtccacc gtggccagcg gccccatgcc gccgaggacg ccgacgacgc
     1801 cgccggcgct cgtcgcggtg cggggatcgg tcatgtcgct cccgggggcg ggcatcgcgc
     1861 aggccatcag cggccggtgg cctgcggcac ggccgcgggc agcgcctgcg ccgcgggggc
     1921 ggcggcgccg cggcgctgct ccaggtgcgc cacgacggcg gtggcgatgc tgttgccgat
     1981 cacgttggtc atcgtgcggc ccatgtccag cagctggtcg atcgccagca cgagggccac
     2041 gccctccatc ggcagccgga acatcggcgc gatcgccgcg accaccacca ccgagccgcg
     2101 cggcacgctg gccatgccct tgctgctgat catcagcacc agcagcatca cgacctgctg
     2161 cgacagcggc atgtcgatgc cgaaggcctg cgcgatgaag atcgccgcga aggactggta
     2221 catcatggag ccgtcgaggt tgaaggcgta gcccagcggc agcgtgaagc cgacgacctt
     2281 cttgtcgatg ccgaagcgct ccagcttctc ggccaggcgc gggaacgccg cctcgctgct
     2341 ggcggtggag aacgcgatca gcgcgggctc gcgcacttcc cggagcagcg cgccgatgcg
     2401 cttgccgagg aagccgtagc ccaccgcgac gagcaccgcc cacaacgcgg ccaggccgac
     2461 gtagaaggtc gacagcaggc ggccgtaggt cacgagcacg tccaggccct tgagcgtgac
     2521 tgccgccgcc agcgcgccga acacgcccag cggggcgaag cgcatgacgt agccggtcag
     2581 cttgagcatc acgggcacca ggccgtcgac ggcgccgatc acggcgtcga cggccgggtt
     2641 gcccttcagg acgttcaggc ccaggccgaa gaacagcgag aacacgacga tctggatgat
     2701 gtcgttgcgc gccatcgcgt cgagcaggct ggtcgggatc gcgtgcgtga cgacgtcgcg
     2761 cacgttcagc cccgaggtgt tcaggccggt gtcgaccgcc gcgccgccgg cggtcatgtg
     2821 caggcccgcg ccgggctgga agaggttggc cagcgccagg cccagcgcga gcgagaccag
     2881 cgacgcgcag gcgaaccagg cgatcgagcg cacgccgatg cgcccgatgt cgcggccgct
     2941 gcccatgccg gccaggcccg agaccagcgt cgagatcacg agcggcgcga tgatcatctt
     3001 gatcagccgc aggaagatgt ccgtgaggat ggcgtagcgg tcggcgaccg ccttcggatc
     3061 ggcggcctgg tggcccagcc acccggccag caccccgagg agcatcccga ggctgatgta
     3121 gagcgtcaat cgatttttca tggcggtcga tgtcgatgag ggcggacccc gcgcgctcgc
     3181 ggggaatccg ggcccgcgcg gtgccacgcg atgcccgtgt cgacattctg gaaatcgccg
     3241 cgtcgccgcg cgatgcggga atcgggaggg gcgatgcggc cgccgcatgc gcggccgcgc
     3301 cgggctcagg cggtgcgtcc cgcgcgccgg tcctgcaggg ccttccacag agtggcgacg
     3361 agcgggttgc gctgggtcgc gtcccgataa accctgatct ccagctccat gcgccagtcg
     3421 ccgccgcccg cggcgatcag ctcgccgcgc gcgagctcgc ccgcgatggc gctgtgcggc
     3481 agccaggcga cgccttcgcc ggcgagcgcc atcttcttca gcagttcggc catgtcggac
     3541 tcgtagtggc gctgcagcgc gggcgcggac cgcgcctgcc ggagcaggaa ctccaggcag
     3601 cggccgaaat agctgccctc ggagtagccg agcagcggcg ccggcctcgc ctgcgcgccc
     3661 gccgacagcc ggaacagcgg gccgccgccg tcggccgcgc ggcataccgg cagcagcgcg
     3721 tcatgcccga gcgagaggtg ctcgtacttc gacggatcga ggtgcagcgg caggctcggg
     3781 tggtggtagg cgaacatcag gtcgcagttc gcgttggcga gcatcacgat cgagtcgtgc
     3841 acgttggtcg ggacgacgcg ggtgcggacc tcgtggaact gcgccggcag ctcgaccagc
     3901 cattgcgaca ggaagttcaa cgcgatggtg tgtcctgccg cgatctgcag cccggggccc
     3961 ggcaggcgcc gatcctcgcg gatcacggcg cgtgcgtcgt agagcttgag cagcacctcc
     4021 tcggccgcct ccttgaacag ccggcccgcc gcggtgagcg agggcgggta ggtgctgcgg
     4081 tcgatcaggt cggcgcccac ccattgctcg agcgcctgga tgcgccggct gaagcccgac
     4141 tgggagacgt tgcgctcctc ggccgcgcgc gagaagctct ggtactgcgc cagggcgacg
     4201 aagtcctcga tccacttgat ttccatgccg gccccgatcc gtcgttggcg cccctcgcgc
     4261 cggattctgc cgcacggccg cggcctgcgg ggcgcccttg accaagcggg acatcgaccc
     4321 ggcgcatgac aaggcctgtc ttgagagcct gaagccgggt cgcaagactg ccatgaacgg
     4381 acctacaccg gcgaccggcg tcgagcgatc tttcacgtcg acggactccg gtctgcacgg
     4441 gaaaggcact ggttggccga cgagtgttga catgagtttc cgtggaaact aaactcgcga
     4501 aagtttcctg ggaaacctca tgaaaggcaa gacgagctca ccatcgccga tggaagccca
     4561 cctgggtttc tggcttcggt tcatctccaa ccatgtctcg gctcgcttcc agcagatgct
     4621 agaagccaag ggcgtgagcg tgactgaatg ggtcgcgctg cgaaccttgt gggcgcgcga
     4681 cgagacccgc catggtgaac tgatccaggc cttgggcatg accaagggcg cagcctccaa
     4741 ggtggtctct cgacttgaag aaaaaggcct cgcggaaaga cggttcgcca gcggaagtcc
     4801 tcgggatcaa tccatggtcc tgaccccagc aggcaaggcc atcgtgcccc ttctggcgtc
     4861 cattgccgac gccaacgaca accacttctt cggtcatctc gatgtgcggg aacgccaggc
     4921 tttgatggcc gccatgcagt ccctcgtgca acaccatggg ctgaaggaca tcccaaccaa
     4981 ctgactcctc aggtcatctt ccccaagtca cggaccacca tgaacgaaac cacccgcacc
     5041 gccatccatg cgaccttcga ggcctcgaac aagggccaga ttcacttcgg tgaggtgatc
     5101 gcgcagctga tggccgctca ggtcgagtcg taccacgtcg actaccgatc cggccgcacc
     5161 acttactacc tgcgagacgg cgccacgctc gactatggct tcgaacgacc acaggaagcc
     5221 attccagagg ccttcgacgc gaatgcggtg cgcggggcca tcctgggcgc acaacagggg
     5281 cgtgtgatgt atcccgagtt caagcaactc tcgcagcgcg cgggctgcgt cgggtacacg
     5341 gtctggatcg caggacgaca tgtcgcctat ctcggccgcc ggggcgagac ccacatcgag
     5401 cactttccaa actgatccgc gaaacccctc acctctcagg aacccacgcg ctgcttcggt
     5461 ctgaacggta gtttcaaggc tgccaagcac gcccttcgag cgtcgctgaa gggtcggatg
     5521 ctgcggcaca caaccgcggg aaccccggcg gcaaccgacc tggcaacggc cctcggccgc
     5581 gtgaccgtcc gctccgcacc tgccgaggcg gcgggccggc acgctcatcg ccgctgcgcg
     5641 ttcactccgg ccactgcggc gtctccccgc tgtcgtcgaa gcgcgggaag atcgtcacgt
     5701 tggcgatgcg gcccttcgcg acccagaaca tcaggccgtg ctgctcgctc tggaagcact
     5761 ggccggcgtc ggcgaagtcg tcggtgagct cgaggtcgtg gatgtcggcc tcgcgggcga
     5821 ggcgggcgag ttcctcgacc ggctggccga tgatcgccac gccgttgacg gtggtcgtgc
     5881 tcgaccaggc ggtgatcgac tcgagacggg catcggcggc ctggtcgaac tcgagctcga
     5941 tctcgccgcc ctggtagaac cagctctcga cgacgctgcc gtcctcgtcc tgcgaggtct
     6001 cgaccgagtc gggccggccg gcggcggcgt ggacggtctc gcggctcatg ccgagcacga
     6061 aggggctgat tccgaagaag ggcgtggcgt cggccatggg gtcgcggcgg tggggggctc
     6121 gtgcggctgc agccctcagt atgtacgacc gccgcggtac tgtgcatgct gccggcgcgc
     6181 gagcgtcgtc gcggcgccga gcgcggccat ggtgcggccg gcatgggcat gcatgatcgc
     6241 gaggccgagg gcgtcggcgg cgtcggtccc aggcaggccg ggcagcgcga ggagccgcgc
     6301 gaccatctcc tgcacctgcg ccttcttcgc gccgccgctg ccgccgacgg ccttcttcat
     6361 ctgcgcggcg gtgtactcgg ccacgggcag gccggccgag accagcgccg cgagcgccgc
     6421 accgcgcgcc tggccgagca gcaggctgct ctgcggactc acgttgacga acacgatctc
     6481 gagggccgcg tgctcgggcg cgtagcgctg cgtgacctcg cgcacgccgt cgaacagcgt
     6541 ccggatgcgg cccggcaggt cgcccttggc catcttcgcg gtcgagatcg tgccgctggc
     6601 cacgtagcgc agcgccgcgc cttccgactc gatcacgccg aagccggtgg tgttcaggcc
     6661 ggggtcgatg ccgaggatgc gcatcagccg tgggcctgga agaaccagcg cgcggagtgg
     6721 aagaacacgg gcgcggcgaa gcacagcggc gcgacgcggt cgagcaggcc gaccgcgccg
     6781 gtgaccgcgc cgctgttgcc ccagtagttg acgccggcgt cgcgcttgag cgcggtcatc
     6841 acgaactcgc cgagcgtgcc ggccgcgccg gcgatgaaag ccatcgccat cgcctgcggc
     6901 agcttgaacg gcgtgatcca gaacagcagc ccgccgacga cggccgcgcc gaagcaggcg
     6961 accgcccacg actgcaggcc gaagcggcgg tcgatcagcc gcgcgacggg gcggcggcgc
     7021 aggcggtggc tcgcgatgtc ctcgaggatc tgcgccgccg tcgtcaccac gacgaggaag
     7081 aacacgagga aggccccgcg gtcgtgggcg tgcggcacct gcagcagcag cagcgcgggg
     7141 gcatgcgaca gcccgtacac gcacaccatc acgccccact ggatcttggc gttgcgctcg
     7201 aggaagcgca gcgggtcgcc gcccagcgcg ctgagcaccg ggatcgcgag gaatacgtag
     7261 accggtatga agacggtgaa caggtcgaag ctctggctgc ccgccagcac gaactgcacc
     7321 ggcagcacga cgaagaacgc gagcatcagg ctgcgatggt cggcgcggcg cgtctgcatc
     7381 agcgtgacga actcgcgcag cgcgaggaag gagacgatcg cgaacaggat cgtcgcaccg
     7441 cccgcgccca tgacccagga cagccagaag atcaccgcgc cgatccaggt cgcgcgcagg
     7501 ttggcctcga aggcggtcag gccgcggcgg ctggcggcgt ggcgctcgcg catcgcgacg
     7561 agcaccgtcc ccgccgagac gagcagcagc ccgccgaaca gcagcaggaa cagcagcccg
     7621 atctgctgct gcgccgtcag tgccttgagg gccatcatga cgcgtgctcc agcgccgcgg
     7681 cgccgcccgg cttgagcgcg acgacggcgg cgcgcgcgcg ggcgaggaag tcggccttgg
     7741 tctcgtcggg cgcgaggttg cacggcgcgc cgaaggtcac cgtgcacagc accgggacgg
     7801 gcacgatctc gcccttgggc atcacgcgct gcacgttgtc gacccaggcg gggatcagct
     7861 gcacgtgcgg gaacttgcgc gcgaggtggt agagcccgct cttgaactcc tggggcaggc
     7921 ccttgttgct gcgcgtgcct tccgggaaga tcacgagcga gtcgccctgc tcgagcgcgg
     7981 cgaccagcgg cgcgagcgga tccgaggacg gatccttgcg ctcgcggtcg acgtagaccg
     8041 cgcggaagac ctcgcgcgtg agccagtgct tgatcttgcc ggtcgtccag tagtcggcgg
     8101 ccgcgatcgg gcgcgtctgc tggcgcaggt cggcgggcag cgtggcccag atcagcaccc
     8161 agtcgaggtg gctctggtgg ttggcgaagt agatgcgttg ctcggccttc ggcgccacgc
     8221 ccttccagtg accctgggcc ccggtgatca ggcgcgcgac ggccgcgagc acgaagcccc
     8281 atgcgtcggc agcgttcatg cgcggactgt accgtctggc cgcgcgcgcg gcgtcgggga
     8341 cgtccctcgc cgcgtgcccc cgcgtgcgaa gctcaacgtc gcgagggcgc gagcgccggc
     8401 gccgagcgcc gcgcgcgcag caccagcacg acgcccagca cgacgaaggg cacgctgagc
     8461 cactggccca cggtgaaggc ctggccggcc tcgtaggcgg cctgcggcgt cttccacgtc
     8521 tcgatcacga tgcgggcggc gaagaccagc gccatgaaca ggccggtgag ctcgccgcgc
     8581 cggcgcagcg cgcggccgcc gcgatccacg gccagcagca ccgccagcac cgccaaatag
     8641 gccgcggcct cgtacagctg cacggggtgg cgcggcagcg cgtcgacgcg ctcgaagacc
     8701 acgccccagt cgccgccggt cgggttgccg acgatctcgg agttgaggaa gttcgcgcag
     8761 cggatgatcg cgccgcccag cgccgccgag ggcgcggcga cgtccagcag ggtcagcagc
     8821 ggcaggcccg gcgcgtagcg cggcgcgccc caggcgagcg cggcgatcag gccgagcgcg
     8881 ccgccgtggc tggccatgcc gccctcccag atcgcgagga tcttcagcgg atgcgccagg
     8941 tagaacgccg ggtcgtagaa caggcagtgc gcgagccgcg cgccgaccac cgtgccgaac
     9001 agggcgcaga tgatcagccc cgagacgtcg acgtcgtgcc gcccgaggcg ggcgagcatg
     9061 cgccgcacgc cgaactcggc gccgaagaag gcgaggcccc agcagatgcc gtaccagtgg
     9121 atggcgaccg ggcccagttg cagcgcgatc gggtcgaagt tccagtgcag cagcatcggc
     9181 gtggcgcgtt ccctgtcgat cagtggcgga agtggcgggt gccggtcagc accatcgcga
     9241 tcccgcgctc gtcggcggcg gcgatgactt cctcgtcgcg catcgagccg cccggctgga
     9301 tgacgcaggt cgcgccggcg tcgcagacga cgtccaggcc gtcgcggaac gggaagaagg
     9361 cgtcgctcgc gaccgccgag ccctgcagcg acaggttggc gttggtcgcc ttgatgccgg
     9421 cgatgcgcgc ggagtcgacg cggctcatct ggcccgcgcc cacgcccagc gtcatgccgc
     9481 cggcgcagaa gacgatcgcg ttgctcttga cgaacttggc gaccttccac gcgaacatca
     9541 ggtcgtccat ctgcgccggc gtcggctgca ccttggtgac gatgcgcagc tccgacggct
     9601 gcacgttgcg cgcgtccgag gtctgcagca gcacgccgcc gccgacgcgc ttgaagtcga
     9661 tcgcgttcat cacgcgctcg gcgggcaacg gcacctcgag caggcgcacg ttctgcttgg
     9721 gcgcgaagac ggcgtgcgcc tcgggcctga tggccggcgc gatcacgacc tcgacgaact
     9781 ggcgcaccac cgcctcggcc gcggcgcggt cgagcgggcg gttgaacgcg atgatgccgc
     9841 cgaaggccga ggtcgggtcg gtcttgaacg ccttcgagta ggcctcgtgc gcgtcggcgc
     9901 cgacggccac gccgcacggg ttggcgtgct tgacgatcac gcaggccggc acgtcgaagg
     9961 tcttgacgca ctcccacgcg gcgtcggcat cggcgatgtt gttgtacgag agctccttgc
    10021 cctgcagctg cgtccacgcg gcgagcgtgc cgggcgcggg gctggcgtcg cggtagaacg
    10081 ccgcgccctg gtgcgggttc tcgccgtagc gcatgtcctg caccttcgtg agctgcaggt
    10141 tgaaggtcgc cggccagtcg ccgcgggcgg gcacggccgc gccgtcgttc tccgcgtccg
    10201 cggccagcgc ggtcaggtag ttggtgatca tgccgtcgta ggcggcggtg tgcgcgaaca
    10261 ccttcttggc gagcgcgaac ttggtcttcc tctgcagccc gcccgccgac agctccgaca
    10321 gcacctgcgg gtaatccgcc gggtcgatca ccacgcccac gtcctgccag ttcttggcgg
    10381 cggcgcgcag catcgcgggg ccgccgatgt cgatgttctc gatcgcgtcc tccagcgtgc
    10441 agtccgggcg cgcggtggcc ttggcgaagg gatagaggtt gatgacgagc aggtcgatgg
    10501 tgccgatgcc gtgctccttg agcgcggcca tgtgctcggg cacgtcgcgg cgcgcgagca
    10561 ggccgccgtg cacgcgcggg tgcagcgtct tcacgcggcc gtccagcatc tccgggaagc
    10621 cggtgacctc ggagacctcg gtcacgggca ggccgctctc ggccagcagc ttggcggtgc
    10681 cgccggtgga cagcagcttc acgccctgcg catgcagcgc gcgggcgaag tcgacgatgc
    10741 cggtcttgtc ggaaacggag agcagggcgg tcagggacat gggtgttctc gggcggaagg
    10801 gaatggattc gtcaggggga tcgtcgagtg ccgggggccg atgctcactg gtcgacgagc
    10861 cggtgctcga gcagcttgcg ccgcagcgtg ttgcggttga tgccgagcca ttcggcggcc
    10921 ttgctctggt tgccgtcggc gtggcgcagc acgacgtcga gcagcggctt ctcgacggtg
    10981 gcgaggaaca tctcgtgcat cgagtgcggc tcggcgccct tgaggtcctt gaaatactgc
    11041 tcgagcgtct cgcggacgca ggcttcgatc tgcttcttgc tcatcgttgt tgttcccggc
    11101 cctcaggctt cttgttgcat cagaccctcg ttggccgccg gccacagcac gtggcggtcg
    11161 gcgagccggt cgaagtagtc cgtcacgccc gccagctgcg tcgcgcagtc ggcgtgcgtg
    11221 ttcatcaccg cgcggaactc gggccctccc ggcagcgcgt gcaccgccca accgatgtgc
    11281 ttgcgggcgg tgcggacgcc gaggtcctcg ccgtacaggc cgtagtggtc gtgcaggtgt
    11341 tcggtgagcc agcgcgcgac gtcgcgcgtg gccggcggcg ggagcagctg ccccgtcgcg
    11401 aggtagtggg cgatctcgcg gaagatccac ggccggccct gtgcggcacg gccgaccatg
    11461 atcgcgtcgg cccgggtgtg cgcgagcacg gcggcggcct tgcgcggcga gtcgatgtcg
    11521 ccgttggcga cgaccgggat gcgcagcgcg gccttgaccg cggcgaccgt gtcgtactcg
    11581 gcgaagccct tgtagccctg ctcgcgcgtc cggccgtgca cggtgaccat cgcgacgccc
    11641 gcggactcgg cggcgcgcgc gagccgcacg gcgttgcgct ccccgtcgca ccagccggtg
    11701 cgcatcttga gcgtgacggg cacgccgcgc ggcgcgcagg cggcgaccac cgcctcgacg
    11761 atgccgatcg cgagcggctc gtcctgcatc agcgcggagc cggcccactt gttgcagacc
    11821 ttcttggccg ggcagcccat gttgatgtcg atgatctgcg cgccgcggtc gatgttgtac
    11881 gcggcggcct cggccatcat ctgcggctcc gtgccggcga tctgcaccga gatcggcgcc
    11941 gtctcgccgt cgtggttggc gcgccgcgag gtcttcagcg aattccacag gtccttgcga
    12001 gaggtgacca tctcgctgac cgcgtagccc gcgccgagcc gcttgcagag catgcggaac
    12061 ggccggtccg tgaccccggc catgggggcg acgaacaggg cattcggcag ctcgatcgcg
    12121 ccaatgtgca tggtggtggg cggggaggaa gggcgggctt gcggatccgc ggcggagcgg
    12181 cccgccagcg ccgcgcccgg gacggcgggc ggcggctgct gctgaaaaac gaggcagaag
    12241 tataaacgct ggcggccgcg ggcggcctgt ccgtccgcgt tcccggagtg ccggccgcgc
    12301 gcgccccgac aatgtcgtga tggatgactg gttttcgccg gtgctgcact ggctcgcgct
    12361 gccgcacaac gggctgccgg cgctgttcgt ggtggccctg gtgtcggcga cgctgctgcc
    12421 gctgggctcg gagcccgcgc tggtcgggct gctgcagctc gacccggggc tgttctggtg
    12481 ggcgatcctg gtcgcgacgg ccggcaacac ggcggggggt gcgatcacct gggccaccgg
    12541 ccgctacgcg cacgtcgcct ggtcgcgcgt cgcccaccgg gcgccgctcg aggcgaagca
    12601 tccgcgcctg gcgggctggc tgcagcgctg ggggccggcg gcctgcgtgc tgggctggct
    12661 accggtggtc ggcgacccgc tgtgcgcggt ggccggctgg ctgcgcatgc cgttctggcc
    12721 ctgcgtcgcg ttcatggcga tcggcaaggg cgcgcgctac gtggcggtcg ccgggctcgc
    12781 gcgcgcggcg ctgcattgag cgccggcgcc ggcggcgaag agagaagggc ccgcgacttt
    12841 cgccgcgggc ccacgcgggt gggaggcccg gcgtaatcga ggcgcagccc ggggcggccg
    12901 cgcgcggcat tcggtgacgg ggacgagtgt cgtcgccctc gcggcggcgc ggcaatccca
    12961 ggaaaagggg atggcggcat cccgccgggg catcggggaa atcgggaggc gggcggctcc
    13021 gccgggcggg
//
LOCUS       DASVLN010000965         4798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_105330, whole genome shotgun sequence.
ACCESSION   DASVLN010000965 DASVLN010000000
VERSION     DASVLN010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105330"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="VF453_22710"
     CDS             complement(<1..158)
                     /locus_tag="VF453_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX7690533.1"
                     /translation="MPTTPSSPPPRSTYRHGDLRRALLEAGIALARDGGPDAVVLREA
                     TRRAGVVP"
     gene            235..672
                     /gene="yjdN"
                     /locus_tag="VF453_22715"
     CDS             235..672
                     /gene="yjdN"
                     /locus_tag="VF453_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HEX7690534.1"
                     /translation="MQATPYLFFEGRCEQAVEFYKKALGAQVHMLLRYKDAPPMPEGA
                     AEGCPGGMPPPADAVMHCALSIGETAIMCSDGMASGKPNFAGFSLSITARDNAHAAQL
                     FDALATGGEVRQPLQETFFAERFGMVADPFGVGWMIIAGQKNP"
     gene            734..1429
                     /locus_tag="VF453_22720"
     CDS             734..1429
                     /locus_tag="VF453_22720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010118178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX7690535.1"
                     /translation="MTSDLKPASELAAAARKPYPNDSAGYERARTALLAEEIELRRHI
                     ERVAARRRALPPGGEALACPLRDANDRTLTLADAFGGNDTLVSYFWMFGPKRPRPCPM
                     CTAFLDAFDQPVRAIRQRVPFVVLGRSPVARQLEFARERGWRNLVFYQVLDDDFARGY
                     RALAPDGGEWPALDVWTKSGDTVRHFWGSELFGTQDPGQDPRGAPDPTPLWNILDLTP
                     AGRGTDWYPALEG"
     gene            complement(1440..2234)
                     /locus_tag="VF453_22725"
     CDS             complement(1440..2234)
                     /locus_tag="VF453_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7690536.1"
                     /translation="MASPSSSRPLAVVTGASSGIGWELARLAAEDGHDLVICADQGPL
                     EEAAERFRALGVAVDAVECDLSTAFGVDKLILAIDARPVDLLMANAGHGLGHGFLDKP
                     FSQARHVIDTNVTGTLDLIHRVGREMRRRRRGRILITGSIAGLTPGSFQAVYNGTKAF
                     VDSFSFALRNELKDTGVSVTCLMPGATATNFFARADMLDTRLAQADKASPAEVAQDGY
                     DAMMKGQDHVVSGWRNKAEAAMTRVLPDSVLAEQHRRAAEPGSQHH"
     gene            complement(2341..4635)
                     /locus_tag="VF453_22730"
     CDS             complement(2341..4635)
                     /locus_tag="VF453_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX7690537.1"
                     /translation="MAKDEESPHIDPFEHAAGGWTSVREVGYALREAHVPFKGTRVLL
                     KQNKPGGFACVSCSWAKPAHPHPAEFCEHGAKATAWEITKARCTPAFFAEHTLTELEG
                     WSDHDLEMAGRLTAPMRYDPATDRYVEVAWDEAFADIGARLRALEPKSAVFYSSGRAS
                     LETSYMYALMARLYGNNNLPDSSNMCHESTSVGLPRSIGVPVGTVTLDDFEQCDAIVL
                     FGHNVGTNSPRMLHSLQDARERGVPLITFNPLREPGLVRFANPQSPVQMLTPKDTVMS
                     TQYCQLKPGGDLAAIAGLCKALLAMDAEAREAGRPAVLDHAFIEQHTHGFLAFCDFLR
                     GVEWPQVERCSGLARAELEEAARTIAGAKRLMLIYGMGLTQHKTGVANVNMIANLALM
                     GGHVGKPGAGILPVRGHSNVQGQRTVGITEKPELVPMDKLRALYGFEPPKDKGLTTVE
                     TCQGLLDGSVKAIVSLGGNLLRAVPDGPVVESAWRRMPLSVQVATKLNRSHVVHGQVA
                     YLLPCLGRIEIDRQDGREQAVSMEDSTGCMHGSRGVVEPASPQLKSEPAIVAGIAKAL
                     LGDTPRVPWDEWVADYARIRKAIEKTYPEIFHDFEQRMWTPGGFHRPLGARHRVWKTP
                     SGKAEFIAPASLAEDGDFGASGPDVIRLTTLRSFDQFNTTVYGDHDVFRGIHGTRRVL
                     MMNGADIERLGLADGAQVRARTAVDDGSTREVADLRVVRYDVLEGCAAGYFPELNPLV
                     PLWHHAVGSQVPGYKSIPIRVSPM"
BASE COUNT          650 a         1695 c         1740 g          713 t
ORIGIN      
        1 ttgggcacga cgccggcgcg gcgcgtggcc tcgcgcagga cgacggcgtc cgggccgccg
       61 tcgcgcgcga gcgcgatgcc ggcttccagc agggcgcggc gcaggtcgcc gtggcgatag
      121 gtcgaacgcg gcggcgggga actcggtgtc gtgggcatgg cgaaaatttg tggacagcgt
      181 ccattttgcc tgatatcttg tggacatcgt acacaaagca aggagtccac ggtcatgcaa
      241 gccacgccct acctgttctt cgaaggccgc tgcgaacagg cggtcgagtt ctacaagaag
      301 gcgctcggcg cccaggtgca catgctgctg cgctacaagg acgcgccgcc gatgcccgag
      361 ggcgcggccg aaggctgccc cggcggcatg ccgccgccgg ccgatgccgt gatgcactgc
      421 gcgctgagca tcggcgagac ggcgatcatg tgctccgacg gcatggcctc gggcaagccg
      481 aacttcgccg ggttctcgct gtccatcacg gcgcgcgaca acgcgcatgc cgcgcagctg
      541 ttcgacgcgc tggccaccgg cggcgaggtg cgccagccgc tgcaggaaac cttcttcgcc
      601 gagcgcttcg gcatggtcgc cgatccgttc ggcgtcggct ggatgatcat cgccggccag
      661 aagaacccct gaggcgtgcc gcgcgggcag gccgcctgcc cgccgccgca tgtcccgctc
      721 gaggagcctg ccgatgacct ccgacctgaa gccggcctcg gaactcgccg cagccgctcg
      781 caagccctat ccgaacgaca gcgccggcta cgagcgggcg cgcaccgcgc tgctggccga
      841 ggagatcgag ctgcgccgcc acatcgagcg cgtcgccgcc cggcggcgtg cgctgccgcc
      901 gggcggcgag gcgctcgcct gcccgctgcg cgacgcgaac gaccgcacgc tcacgctcgc
      961 cgacgccttc ggcggcaatg acaccctcgt cagctacttc tggatgttcg gcccgaagcg
     1021 cccgcgcccg tgcccgatgt gcacggcctt cctcgacgcg ttcgaccagc ccgtccgtgc
     1081 aatccgacag cgggtgccct tcgtcgtcct cggccgctcg cccgtcgccc gccagctcga
     1141 gttcgcgcgt gagcgcggct ggcgcaacct cgtcttctac caggtgctgg acgacgactt
     1201 cgcccgcggc taccgcgcgc tcgcgccgga cggcggcgaa tggccggcgc tcgacgtctg
     1261 gacgaagtcg ggcgacacgg tgcgccactt ctggggcagc gagctgttcg gcacccagga
     1321 tccgggccag gatccacgcg gcgcgcccga tccgacgccg ctgtggaaca tcctcgacct
     1381 cacgcccgcg ggccgcggca cggactggta tcccgcgctg gaaggctgac gccgcggctt
     1441 caatgatgct gcgagcccgg ctccgcggcg cggcgatgct gctccgccag cacgctgtcc
     1501 ggcaggacgc gcgtcatcgc cgcctcggcc ttgttgcgcc agcccgagac gacgtgatcc
     1561 tggcccttca tcatcgcgtc gtagccgtcc tgggcgacct ccgcgggcga ggccttgtcg
     1621 gcctgcgcga gccgcgtgtc gagcatgtcc gcgcgggcga agaagttggt cgcggtcgcg
     1681 cccggcatca ggcaggtcac gctgacgccg gtgtccttca gctcgttgcg cagcgcgaac
     1741 gagaacgagt cgacgaaggc cttcgtgccg ttgtagacgg cctggaacga gcccggcgtc
     1801 aggccggcga tcgagcccgt gatcaggatg cggccgcggc ggcggcggcg catctcgcga
     1861 ccgacgcggt ggatcaggtc cagcgtgccg gtgacgttgg tgtcgatcac gtggcgcgcc
     1921 tgcgagaacg gcttgtccag gaacccgtgg cccaggccgt ggccggcgtt ggccatcagc
     1981 aggtccaccg ggcgcgcgtc gatcgcgagg atcagcttgt cgacgccgaa cgcagtggac
     2041 aggtcgcatt cgaccgcgtc gaccgccacg ccgagggcgc ggaagcgttc ggcggcctct
     2101 tcgagcggcc cctggtcggc gcagatgacg aggtcgtggc cgtcctcggc ggcgaggcgg
     2161 gcgagttccc agccgatgcc gctggaggcg cccgtcacga cggcgagcgg tcggctggag
     2221 gaaggcgagg ccatggcggg ctccttgaag cgggtggatc gccttcgagg aggcaacgtc
     2281 catgccgcgc cggcgccgtc cgacaccgcg gccgagcgcg cggcccgcgc ccgtgcgcgc
     2341 tcacatcggg ctgacgcgga tcggtatcga cttgtagccc ggcacctggc tgcccaccgc
     2401 gtggtgccac agcggcacga gcgggttgag ctcggggaag tagccggcgg cgcagccctc
     2461 gagcacgtcg tagcggacaa cccgcaggtc ggcgacctcg cgcgtcgagc cgtcgtcgac
     2521 cgccgtgcgc gcgcgcacct gcgcgccgtc ggccaggccc aggcgctcga tgtcggcgcc
     2581 gttcatcatc agcacgcggc gcgtgccgtg gatgccgcgg aagacgtcgt ggtcgccgta
     2641 gaccgtcgtg ttgaactggt cgaagctgcg cagcgtggtc aggcggatca cgtcgggccc
     2701 cgaggcgccg aagtcgccgt cctcggcgag cgaggccggc gcgatgaact ccgccttgcc
     2761 cgagggcgtc ttccacacgc ggtggcgcgc gccgaggggg cggtggaagc cgccgggcgt
     2821 ccacatgcgc tgctcgaagt cgtggaagat ctcggggtag gtcttctcga tcgccttgcg
     2881 gatgcgcgcg tagtcggcga cccactcgtc ccagggcacg cgcggcgtgt cgccgagcag
     2941 ggccttggcg atgccggcga cgatcgccgg ctcggacttc agctgcgggc tcgccggctc
     3001 gacgacgccg cgcgagccgt gcatgcagcc ggtggaatcc tccatcgaca cggcttgctc
     3061 gcggccgtcc tggcggtcga tctcgatgcg gcccaggcag ggcagcaggt acgcgacctg
     3121 gccgtgcacg acgtggctgc ggttgagctt ggtcgccacc tgcaccgaca acggcatgcg
     3181 ccgccatgcg gactccacga cggggccatc gggcacggcg cgcagcaggt tgcccccgag
     3241 gctgacgatc gccttgacgc tgccgtctag cagcccctgg caggtctcga cggtcgtgag
     3301 gcccttgtcc ttcggcggct cgaagccgta gagcgcgcgc agcttgtcca tcggcacgag
     3361 ctcgggcttc tcggtgatgc ccacggtgcg ctggccctgc acgttcgagt ggccgcgcac
     3421 gggcaggatg cccgcgccgg gcttgccgac gtggccgccc atcagcgcga ggttggcgat
     3481 catgttcacg ttggcgacgc ccgtcttgtg ctgcgtgagc cccatgccgt agatcagcat
     3541 cagccgcttg gcgccggcga tcgtgcgcgc ggcctcctcg agctcggcgc gcgcgagccc
     3601 gctgcagcgc tcgacctgcg gccactcgac cccgcgcagg aagtcgcaga acgcgaggaa
     3661 gccgtgcgtg tgctgctcga tgaacgcgtg gtcgaggacg gcggggcggc cggcctcgcg
     3721 cgcctcggcg tccatcgcca gcagcgcctt gcacaggccc gcgatcgccg cgaggtcgcc
     3781 gccgggcttg agctggcagt actgcgtgct catcacggtg tccttgggtg tgagcatctg
     3841 cacgggcgac tgcgggttgg cgaagcgcac caggcccggc tcgcgcagcg ggttgaacgt
     3901 gatgagcggc acgccgcgct cgcgcgcgtc ctgcagcgag tgcagcatgc gcgggctgtt
     3961 ggtgccgacg ttgtggccga acagcacgat cgcgtcgcac tgctcgaagt cgtccagcgt
     4021 caccgtgccc accggcacgc cgatgctcct gggcagcccg accgaggtgc tctcgtggca
     4081 catgttggag ctgtcgggca ggttgttgtt gccgtacagg cgtgccatca gcgcgtacat
     4141 gtacgaggtc tccagcgacg cgcgccccga cgagtagaag acggccgact tcggctccag
     4201 cgcccgcagg cgcgcgccga tgtcggcgaa cgcctcgtcc cacgcgacct cgacatagcg
     4261 gtcggtggcg gggtcgtacc tcatgggcgc ggtcaggcgg ccggccatct cgaggtcgtg
     4321 gtcgctccag ccttcgagct ccgtgagcgt gtgctcggcg aagaacgcgg gcgtgcagcg
     4381 cgccttcgtg atctcccacg cggtggcctt ggcgccgtgc tcgcagaact cggctggatg
     4441 cggatgcgcc ggcttggccc aggagcagct gacgcaggcg aagccgccgg gcttgttctg
     4501 cttgagcagg acgcgcgtgc ccttgaacgg gacgtgcgcc tccctgagcg cgtagccgac
     4561 ctcgcgcacg gaggtccagc cgcccgcggc gtgctcgaac gggtcgatgt ggggggattc
     4621 ctcgtccttg gccatggcgt gcggtctcct tgcgcgggtg ggccgacgcg gcaagacgcg
     4681 ttcccccgcg gccgcgcgca tgcgcacggc ctcgctacac tcggcgcacc cgagccggaa
     4741 gcgctcgccg ccgtcgaccg aaagggcacg ccatgatccg gaagatcccg accgtcct
//
LOCUS       DASVLN010000966         2564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_373588, whole genome shotgun sequence.
ACCESSION   DASVLN010000966 DASVLN010000000
VERSION     DASVLN010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373588"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /gene="mnmE"
                     /locus_tag="VF453_22735"
     CDS             complement(<1..899)
                     /gene="mnmE"
                     /locus_tag="VF453_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX7690538.1"
                     /translation="MTARLAAFDDPIVAIATAPGRGAVGIVRASGRDLSTLVEAVCGR
                     VLMPRVAHYGPFLDARGEAIDQGLALHFPAPNSYTGEDVLELQAHGGPVVLQLILARL
                     LEAGRGIGLRLAQPGEFTSRAFLNDKLDLAQAEAVADLIDASTEAAARSAGRALAGAF
                     SKEIDALAARIVHLRMLVEATLDFPEEEIDFLERAGARRQLAEIDAQLEAVMRRARQG
                     ALLREGLRVVLAGAPNVGKSSLLNALAGAELAIVTPIAGTTRDRVSETIQIHGVPVHV
                     VDTAGLRDASDEVERIGIERSWQA"
     gene            complement(901..>2564)
                     /gene="yidC"
                     /locus_tag="VF453_22740"
     CDS             complement(901..>2564)
                     /gene="yidC"
                     /locus_tag="VF453_22740"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX7690539.1"
                     /translation="VPRPVATAPAKTVVREAGAAGTPAAAASAAAAAAAEFPEQRVTI
                     TTDVVKATLDTRGGDLVDLDLLKYQENDSHSEIVAMAKRLIGIKTDTVKNGDVQVFDT
                     APNQYYVAQTGLSLKDSSRPLPQHTTVMRLASTERTLAPGKDTLALRFESPEIDGVQL
                     VKTYTFHRGSYAVDVTHEVVNHSGAPISPDLYLQLVRDGRVPAGGYFNGPSSYTGPSV
                     YRDGTFHKISFSDIDKGKEKDTVDPKQTLNNGWIAMVQHYFSSAWVLPENAPRSYSFG
                     HFGPEVHGETTYFAAMFTPVGTVAPGASKTVSATLYAGPSEEDLLAPLGLQQVKDYGL
                     LKIVAQPLFSLLSFIHRFVGNWGWSIVVLVFLLKIAFYSLNANAYRSMAKMKAVNPRV
                     QELRERYKDNPQQMQQEMMKIYREEKVNPLGGCLPILIQMPIFIALFTVLTSTVEMRG
                     APWIGWIHDLSIMDPYAILPVLMTLSGLAQVALNPTPADPTQARMMWIMPLIFSAALF
                     SFPSGLVLYYVTNNLLTILQQWLINRQINGPKKKDGVSDAKVVKG"
BASE COUNT          356 a          860 c          929 g          419 t
ORIGIN      
        1 gcctgccagc tgcgctcgat gccgatgcgc tcgacctcgt cgctcgcgtc gcgcagcccc
       61 gccgtgtcca ccacgtgcac cggcacgccg tggatctgga tcgtctcgct gacgcggtcg
      121 cgcgtggtgc cggcgatcgg cgtgacgatg gcgagctccg cgccggccag cgcgttcagc
      181 aacgagctct tgccgacgtt gggcgcgccg gcgagcacga cgcgcagtcc ctcgcgcagc
      241 agggcgccct ggcgcgcgcg ccgcatgacg gcctcgagct gcgcgtcgat ctcggcgagc
      301 tggcggcgcg cgcccgcgcg ctcgaggaag tcgatctctt cctcggggaa gtccagcgtc
      361 gcctcgacca gcatgcgcag gtgcacgatg cgggccgcga gggcgtcgat ctccttcgag
      421 aacgcgccgg cgagcgcccg gccggccgag cgggccgccg cttcggtgct ggcgtcgatc
      481 aggtcggcga cggcttcggc ctgggcgagg tcgagcttgt cgttgaggaa ggcgcgcgag
      541 gtgaactcgc ccggctgcgc gagccgcagg ccgatcccgc ggccggcctc gagcaggcgc
      601 gcgaggatca gttgcagcac gaccggcccg ccgtgggcct gcagctcgag gacgtcctcg
      661 ccggtgtagg aattcggcgc cgggaagtgc agcgcgaggc cctggtcgat cgcctcgcca
      721 cgggcgtcga ggaaggggcc gtagtgcgcg acgcgcggca tcagcacgcg cccgcatacc
      781 gcctcgacca gcgtcgacag gtcgcgcccc gaggcccgca cgatgcccac cgcgccgcgc
      841 ccgggcgcgg tggcgatggc gacgatcggg tcgtcgaacg cggcgagccg ggcggtcacg
      901 tcaccccttg acgaccttcg cgtcggacac gccgtccttc ttcttcgggc cgttgatctg
      961 gcggttgatc agccactgct gcaggatcgt cagcaggttg ttcgtcacgt agtacagcac
     1021 caggccggac gggaacgaga acagcgccgc gctgaagatc agcggcatga tccacatcat
     1081 gcgcgcctgc gtcgggtcgg cgggcgtcgg gttgagcgcc acctgcgcga ggccggacag
     1141 cgtcatcagc accggcagga tcgcgtacgg atccatgatg gacaggtcgt ggatccagcc
     1201 gatccagggc gcgccgcgca tctccacggt cgaggtcagc accgtgaaca gggcgatgaa
     1261 gatcggcatc tggatgagga tcggcaggca tccgccgagc ggattgacct tttcctcccg
     1321 gtagatcttc atcatctcct gctgcatctg ctgcgggttg tccttgtagc gctcgcgcag
     1381 ttcctggacg cgcgggttga ccgccttcat cttggccatc gagcggtacg cgttcgcgtt
     1441 caggctgtag aacgcgatct tcagcaggaa caccagcacg acgatcgacc agccccagtt
     1501 gccgacgaag cggtggatga agctcagcag cgagaacagc ggctgcgcga cgatcttgag
     1561 caggccgtag tccttgacct gctgcaggcc cagcggggcg agcaggtcct cctcgctcgg
     1621 gccggcgtac agcgtggccg agacggtctt gctcgcgccc ggcgcgaccg tgccgacggg
     1681 cgtgaacatc gcggcgaagt aggtcgtctc gccgtgcacc tcgggcccga agtgaccgaa
     1741 gctgtagctg cgcggcgcgt tctccggcag cacccaggcc gacgagaagt agtgctgcac
     1801 catcgcgatc cagccgttgt tcagcgtctg cttcgggtcg accgtgtcct tctccttgcc
     1861 cttgtcgata tcggagaagc tgatcttgtg gaaggtgccg tcgcggtaga ccgacgggcc
     1921 ggtgtacgag ctggggccgt tgaagtagcc gcccgcgggc acgcggccgt cgcgcacgag
     1981 ctgcaggtac aggtcggggc tgatcggcgc gcccgagtgg ttgaccacct cgtgcgtcac
     2041 gtcgaccgcg tagctgccgc ggtggaaggt gtaggtcttg accagctgga cgccgtcgat
     2101 ctccggcgac tcgaagcgca gggcgagcgt gtccttgccg ggcgcgagcg tgcgctcggt
     2161 gctcgccagg cgcatgaccg tcgtgtgctg cggcagcggg cggctgctgt ccttgaggct
     2221 gaggccggtc tgcgcgacgt agtactggtt cggcgcggtg tcgaagacct ggacgtcgcc
     2281 gttcttgacg gtgtccgtct tgatgccgat caggcgcttg gccatggcga cgatctcgct
     2341 gtggctgtcg ttctcctggt acttcagcag gtcgaggtcg accaggtcgc cgccgcgcgt
     2401 gtccagcgtc gccttgacga cgtcggtcgt gatggtgacg cgctgctccg ggaactccgc
     2461 cgccgcagcg gccgcggcag aggcggcggc cgctggcgta ccggcggcgc cggcttcgcg
     2521 cacgaccgtc ttcgccggcg ccgtcgcgac gggcctgggc accg
//
LOCUS       DASVLN010000967         6861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_266367, whole genome shotgun sequence.
ACCESSION   DASVLN010000967 DASVLN010000000
VERSION     DASVLN010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266367"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1283)
                     /locus_tag="VF453_22745"
     CDS             complement(12..1283)
                     /locus_tag="VF453_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7690540.1"
                     /translation="MTLSLSARRLAAAATAALACAILPLATAQAAEVEVLHWWTSGGE
                     AKSVEELKRLLGQQGVTWRDFAVPGGGGDAAMTALRARVDAGKPPTAAQIKGPAIQDW
                     GRKGVLANLDDVAQEGDWDVKLPRTIAAQMKVDGHWAAVPVNVHRVNWLWINRDLLRQ
                     VGGRVPENWDQFFALAEKFKQIHVIPLAHGGQPWQDLTTFEVVLLGVGGPEFYQQAFV
                     KMDPAAWRSPTMSRVIDTFRRMKQYTDKDAPGRDWNVATSMVIKGQAAMQIMGDWAKG
                     EFLAAGKKPETDFICTAAPGTWESYIYNIDSFAMFRLKDPQAVQGQKALARTIMSPSF
                     QETFNRNKGSIPVALGANMASFDYCARESSGYFVAASLANSLVPSFAHKMAQPEAKVA
                     AVQAVVAKIWDDDSYAGPRAQADLAAAAAAR"
     gene            complement(1482..2198)
                     /locus_tag="VF453_22750"
     CDS             complement(1482..2198)
                     /locus_tag="VF453_22750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7690541.1"
                     /translation="MSQEILLEAEGLRVAYGGIQAVKGASFQVRAGELVSLIGANGAG
                     KTTTLKAVTGIQPVAGGDIKFLGKSIRGQGAWDLVKQGLIMVPEGRGTFTRMTIVENL
                     LMGAHVRNDDEVEADIEKVFATFPRLKERAQQLAGTMSGGEQQMLAMGRALMARPKVL
                     LLDEPSMGLSPIMVDKIFEVVETIHKQGTTVLLVEQNASRALSIADRGYVMESGEVTM
                     NGEAEMLLNDPKVRAAYLGE"
     gene            complement(2219..2995)
                     /locus_tag="VF453_22755"
     CDS             complement(2219..2995)
                     /locus_tag="VF453_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7690542.1"
                     /translation="MSNAVLQVNGVSKRFGGLQALSDVGITINGGQVYGLIGPNGAGK
                     TTFFNVITGLYTPDSGSFMLGGAPYQPTAVHEVAKTGIARTFQNIRLFAEMTAIENVM
                     VGRHVRTNSGLFGAVFRSGHFKKEEAEIRQRSQELLDYVGIGRFAEYKARTLSYGDQR
                     RLEIARALATDPKLIALDEPAAGMNATEKVVLRELIDRIRNDGRTILLIEHDVKLVMG
                     LCDRVTVLDYGKQIAEGTPADVQRNEKVIEAYLGAGHKPH"
     gene            complement(3011..4096)
                     /locus_tag="VF453_22760"
     CDS             complement(3011..4096)
                     /locus_tag="VF453_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7690543.1"
                     /translation="MKNKFVTLVLGLAALALLPFFAQYFGNGWVRILDTALLYMMLAL
                     GLNVVVGYAGLLDLGYIAFYAVGAYLFGELASPHLTDTFDSFKRAFPNGLHISIWLIV
                     PLGALVAGTFGVLLGAPTLRLRGDYLAIVTLGFGEIVRVFMNNLDAPVNITNGPKGLD
                     SIDSIHLLGMDLGKDHTVAGIEVHSVTQYYFLFLLLVAFAVLVCYRLERSRIGRAWMA
                     IREDEIAAKAMGINTRNMKLLAFGMGATFGGVSGVMFGGFQGFVSPESFTLPESVMVV
                     AMVVVGGIGHIPGVLLGALLLSALPEVLRYVADPLQEMTGGRLDAAILRQLLISLAMI
                     VVMLLRPRGLWPSPEHGKASPAPATVK"
     gene            complement(4123..5046)
                     /locus_tag="VF453_22765"
     CDS             complement(4123..5046)
                     /locus_tag="VF453_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7690544.1"
                     /translation="MDIFIQQIINGLVLGSMYALVALGYTMVYGIVNLINFAHGEVLM
                     VGALTSWSVTEVLANAGLPGWLLLLVALIAAIVVCSILNFTIEKVAYRPLRNAPRLAP
                     LITAMGMSLLLQTLAMIIWKPNYHSYPSLLPHDPIFIGGAVTNFTQITILVVTAITLF
                     GLMWLVNKTKLGRAMRATAENPRVAALMGVRPDNVISATFVIGAALAALAGVMYASNY
                     GTAQFSMGFLPGLKAFTAAVFGGIGNLAGAMVGGVLLGLIEALGSGYIGDLTGDVLGS
                     NYQDIFAFIVLILVLTLRPKGLLGERVADRA"
     gene            complement(5353..6702)
                     /locus_tag="VF453_22770"
     CDS             complement(5353..6702)
                     /locus_tag="VF453_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX7690545.1"
                     /translation="MDDLFKLDPAAPLAESLRPKTIAEVIGQSHLLGEGRPLRLAFQS
                     GKPHSMIFWGPPGVGKTTLARLTATAFDCAFIALSAVFSGVKDIRAAMEQAQVNQAHG
                     RHTILFVDEIHRFNKSQQDALLPYVENGLVTFIGATTENPSFEVNSALLSRAQVYVLK
                     PLTDDELKALLARAQAGALHGLEFDELARDTLVGYADGDARRLLNLLEQTGTAAGAAG
                     VTRIDADFVQNALTLNARRFDKGGDNFYDQISALHKSVRGSSPDGALYWLCRMLDGGA
                     DPRYLARRIVRMAWEDIGLADPRAMQVANDAATTYERLGSPEGELALAQAVIYLAVAA
                     KSNAGYNAYNAARAFVKSDKSREVPVHLRNAPTRLMKELGYGHEYRYAHDEPNAYAAG
                     ETYLPDGMPDPRWYRPVPRGVEAKIADKLAQLRRWDDEARRGEPPAPADAELPGEEP"
BASE COUNT         1049 a         2299 c         2399 g         1114 t
ORIGIN      
        1 gcccgcggcg gtcagcgcgc cgccgcggcc gcggcgagat ccgcctgcgc gcgcgggccg
       61 gcgtagctgt cgtcgtccca gatcttcgcg accacggcct gcaccgcggc gaccttggcc
      121 tccggctgcg ccatcttgtg cgcgaacgac ggcaccagcg aattggcgag cgaggcggcg
      181 acgaagtagc cgctggactc gcgcgcgcag tagtcgaagg acgccatgtt ggcccccagc
      241 gcgaccggga tcgagccctt gttgcggttg aaggtctcct ggaagctcgg gctcatgatc
      301 gtgcgcgcga gcgccttctg cccctgcacg gcctgcggat ccttcagacg gaacatcgcg
      361 aagctgtcga tgttgtagat gtaggactcc caggtgccgg gcgcggcggt gcagatgaag
      421 tcggtctcgg gcttcttgcc ggcggcgagg aactcgccct tggcccagtc gcccatgatc
      481 tgcatcgccg cctggccctt gatcaccatc gaggtggcga cgttccagtc gcggcccggc
      541 gcatccttgt ccgtgtactg cttcatgcgg cggaaggtgt cgatcacgcg cgacatcgtg
      601 gggctgcgcc atgccgccgg atccatcttc acgaaggcct gctggtagaa ctcggggccg
      661 ccgacgccca gcagcacgac ctcgaaggtg gtcaggtcct gccagggctg gcccccgtgc
      721 gcgagcggga tgacgtggat ctgcttgaac ttctcggcca gcgcgaagaa ctggtcccag
      781 ttctccggca cgcggccgcc gacctggcgc aggaggtcgc ggttgatcca cagccagttg
      841 acccggtgca cgttgaccgg caccgccgcc cagtggccgt cgaccttcat ctgcgcggcg
      901 atcgtgcgcg ggagcttgac gtcccagtcg ccttcctgcg cgacgtcgtc gaggttggcg
      961 agcacgccct tgcggcccca gtcctggatc gccgggccct tgatctgcgc cgccgtcggc
     1021 ggcttgccgg cgtcgacgcg cgcacgcagc gccgtcatcg cggcgtcgcc gccgccgccg
     1081 gggacggcga aatcccgcca ggtcacgccc tgctggccga gcagccgctt gagctcctcg
     1141 accgacttcg cctcgccacc cgaggtccac cagtgcagga cctcgacttc ggccgcctgg
     1201 gcggtcgcga ggggcaggat ggcgcaggcg agcgccgccg tcgcggcggc tgcgagccgc
     1261 cgcgcgctca gggagagggt catgcgttgt ctccaccgga gccgctcgct ggccgggatg
     1321 gctgcggcgc tctcggaact ttttatgaaa tcaacgataa cccatcgtta atggatgaac
     1381 agatcctgaa acgcaaatct ttcactatcc cggccgcttc gatgcgcggc ccgccatgaa
     1441 aaaggcgccc gcaggcgcct tcctcgaacg gcgggccagc ctcactcgcc caggtaggct
     1501 gcgcgcacct tgggatcgtt cagcagcatc tcggcctcgc cattcatcgt gacctcgccc
     1561 gactccatca cgtagccgcg gtcggcgatc gacagcgcgc ggctggcgtt ctgttccacc
     1621 agcaggacgg tcgtgccctg cttgtggatg gtctcgacga cttcgaagat cttgtcgacc
     1681 atgatcggcg acaggcccat cgacggctcg tccagcagca gcaccttggg acgcgccatc
     1741 agcgcgcggc ccatggccag catctgctgc tcgccgccgg acatcgtgcc ggcgagctgc
     1801 tgcgcgcgtt ccttcaggcg cgggaaggtg gcgaagacct tctcgatgtc ggcctcgacc
     1861 tcgtcgtcgt tgcgcacgtg cgcgcccatc agcaggttct cgacgatggt catgcgcgtg
     1921 aacgttccgc ggccctcggg caccatgatc aggccctgct tgaccaggtc ccacgcgccc
     1981 tggccccgga tcgacttgcc gaggaacttg atgtcgccgc cggccaccgg ctggatgccc
     2041 gtgaccgcct tgagcgtcgt ggtcttgccc gcgccgttgg cgccgatcag gctgaccagc
     2101 tcgccggcgc gcacttggaa ggacgcgccc ttgaccgcct ggatgccgcc gtaagcgacc
     2161 cgcaggcctt ccgcctcgag gaggatttct tgactcatgt tgcgtgtgct cctcgtgctc
     2221 aatgcggctt gtggccggcg ccgaggtagg cctcgatcac cttctcgttg cgctggacgt
     2281 cggcgggcgt gccctcggcg atctgcttgc cgtagtccag gacggtcacg cggtcgcaca
     2341 ggcccatcac cagcttgacg tcgtgctcga tcagcaggat ggtgcggccg tcgttgcgga
     2401 tgcggtcgat cagctcgcgc agcacgacct tctcggtggc gttcatgccg gcggcgggct
     2461 cgtccagcgc gatcagcttc gggtcggtcg cgagcgcgcg ggcgatctcg aggcggcgct
     2521 ggtcgccgta ggacagcgtg cgcgccttgt actcggcgaa gcggccgatg ccgacgtagt
     2581 ccagcagctc ctgcgagcgc tggcggatct cggcctcctc cttcttgaag tgcccggagc
     2641 ggaagaccgc gccgaacagg cccgagttcg tgcgcacgtg gcggccgacc atcacgttct
     2701 cgatcgccgt catctcggcg aacaggcgga tgttctggaa cgtgcgcgcg atgccggtct
     2761 tggcgacctc gtgcaccgcg gtcggctggt agggcgcgcc gcccagcatg aagctgccgg
     2821 aatcgggcgt gtacaggccg gtgatgacgt tgaagaaggt ggtcttgccg gcgccgttgg
     2881 ggccgatcag gccgtagacc tggccgccgt tgatcgtgat gccgacgtcg gacagggcct
     2941 gcaggccgcc gaagcgcttg gagacgccgt tgacctggag aactgcgttg ctcatggtgc
     3001 ggagcttcct ttacttgacg gtcgcggggg cgggactggc cttgccgtgc tccggcgacg
     3061 gccacaggcc gcgtggccgc agcagcatca cgacgatcat cgccagcgag atgagcagct
     3121 ggcgcaggat ggccgcgtcg aggcgcccgc cggtcatctc ctgcagcgga tcggcgacgt
     3181 agcgcagcac ctcgggcagg gccgacagca gcagcgcgcc gagcagcacg ccggggatgt
     3241 ggccgatgcc gccgacgacg accatcgcca ccaccatcac cgactccggc agcgtgaacg
     3301 actcgggcga gacgaagccc tggaagccgc cgaacatcac gcccgagacg ccgccgaagg
     3361 tcgcgcccat gccgaacgcg agcagcttca tgttgcgggt gttgatgccc atcgccttgg
     3421 cggcgatctc gtcctcgcgg atggccatcc aggcgcggcc gatgcgcgag cgttccaggc
     3481 ggtagcagac gagcacggcg aacgccacca gcagcaggaa caggaagtag tactgcgtca
     3541 ccgaatggac ctcgatgccg gcgaccgtgt gatccttgcc gagatccatg cccagcaggt
     3601 ggatcgagtc gatcgagtcc aggcccttgg gaccgttggt gatgttgacc ggcgcgtcca
     3661 ggttgttcat gaacacgcgc acgatctccc cgaagccgag ggtgacgatg gcgaggtagt
     3721 cgccgcgcag gcggagggtc ggcgccccca gcagcacgcc gaaggtgccc gcgaccagcg
     3781 cgcccagcgg cacgatcagc cagatcgaga tgtgcaggcc gttcgggaac gcgcgcttga
     3841 acgagtcgaa cgtgtcggtc aggtggggcg acgcgagctc gccgaacagg taggcgccca
     3901 ccgcgtagaa cgcgatgtag cccaggtcga gcaggccggc gtagcccacg acgacgttca
     3961 ggcccagcgc cagcatcatg tacagcagcg cggtgtcgag gatgcgcacc cagccgttgc
     4021 cgaagtactg cgcgaagaac ggcagcagcg cgagcgccgc gaggccgagg acgagggtga
     4081 cgaacttgtt cttcatcggg tgttctcctg gttcgtcgcg ggtcaggcgc ggtcggccac
     4141 gcgctcgccg agcaggccct tcgggcgcag cgtcagcacg aggatcagca cgatgaacgc
     4201 gaagatgtcc tggtagttgg agcccagcac gtcgccggtg aggtcgccga tgtagccgct
     4261 gcccagcgcc tcgatcaggc ccagcaggac gcccccgacc atcgcgccgg cgaggttgcc
     4321 gatgccgccg aagacggccg ccgtgaacgc cttcaggccg ggcaggaagc ccatcgagaa
     4381 ctgcgcggtg ccgtagttgg aggcgtacat cacgccggcc agcgcggcca gggcggcgcc
     4441 gatcacgaag gtggcggaga tgacgttgtc cggccgcacg cccatcagcg cggcgacgcg
     4501 cgggttctcg gcggtggcgc gcatcgcgcg gcccagcttg gtcttgttga ccagccacat
     4561 caggccgaac agcgtgatcg cggtgacgac caggatggtg atctgcgtga agttggtgac
     4621 ggcgccgccg atgaagatcg ggtcgtgcgg cagcagcgac gggtacgagt ggtagttcgg
     4681 cttccagatg atcatcgcga gcgtctgcag cagcaggctc atgcccatcg cggtgatcag
     4741 cggggccagg cgcggcgcgt tgcgcagcgg gcggtaggcg accttctcga tcgtgaagtt
     4801 caggatcgag cagacgacga tcgcggcgat cagcgcgacc agcagcagca gccatccggg
     4861 caggcccgcg ttggcgagca cctcggtgac cgaccagctg gtcagggccc ccaccatcaa
     4921 cacttcgccg tgcgcgaagt tgatcaggtt gacgatgccg taaaccatcg tgtagccgag
     4981 tgccacgagc gcatacatgc tcccgagcac cagcccattg atgatctgct gaatgaagat
     5041 atccatcagg ggtctcctgg atcttgaccg ccgcgcgcga tctcgggtcg cgcgcgggcc
     5101 ctcacgtttc ggcgccggcc gtcgcggggg cggcgggcag gcgcccggcg ggggtcaccc
     5161 ccactcgttg gcgggattct agaagtcccc tgccgcacaa gcggacggac ttacccgcgt
     5221 agaaccgaac gacccactat tgacgaggcc gaggaacggg ctttcttcgc cggagagtcg
     5281 tgttggacgg agttcggtga actttgcccg gaggccgcac tcgaaggcgc gccggcaggc
     5341 gtcgaggccg ggtcagggct cttcgcccgg aagctcggcg tctgcgggcg cggggggttc
     5401 gccgcggcgc gcctcgtcgt cccagcggcg cagctgggcc agcttgtcgg cgatcttcgc
     5461 ctcgaccccg cgcggcaccg gccggtacca gcgcggatcg ggcatgccgt ccggcaggta
     5521 ggtctcgccc gcggcgtagg cgttgggctc gtcgtgggcg tagcggtact cgtggccgta
     5581 gccgagctcc ttcatcagcc gcgtcggtgc gttgcgcagg tgcaccggca cctcgcggct
     5641 cttgtcggac ttcacgaacg cgcgcgccgc gttgtatgcg ttgtagccgg cgttgctctt
     5701 ggccgccacg gccaggtaga tcacggcctg cgccagcgcg agctcgcctt cggggctgcc
     5761 gaggcgctca taggtcgtcg cggcgtcgtt ggccacctgc atggcgcggg gatcggccag
     5821 tccgatgtcc tcccacgcca tgcgcacgat gcggcgggcg aggtagcgcg ggtcggcgcc
     5881 gccgtcgagc atccggcaca gccagtacag cgcgccgtcc gggctggagc cgcgcacgga
     5941 cttgtggagc gccgagatct ggtcgtagaa attgtcaccg cccttgtcga agcggcgcgc
     6001 gttcaacgtc agcgcgttct gcacgaaatc ggcgtcgatg cgcgtgacgc ccgcggcgcc
     6061 ggccgccgtc ccggtctgct ccagcaggtt gagcaagcgg cgcgcgtcgc cgtccgcgta
     6121 gccgacgagc gtgtcccggg cgagctcgtc gaattcgagc ccgtgcagcg cgcccgcctg
     6181 cgcccgcgcg agcaaggcct tcagctcgtc gtcggtcagc ggcttgagca cgtagacctg
     6241 cgcccgcgac agcagcgcgg agttgacctc gaacgacggg ttctccgtcg tcgcgccgat
     6301 gaaggtgacg aggccgttct cgacgtaggg caggagcgcg tcctgctgcg acttgttgaa
     6361 gcggtggatc tcgtcgacga acaggatcgt gtgccggccg tgggcctggt tgacctgcgc
     6421 ctgctccatc gcggcgcgga tgtccttcac gcccgagaac acggccgaca gcgcgatgaa
     6481 ggcgcagtcg aacgcggtcg cggtgaggcg ggcgagcgtc gtcttgccca cgcccggcgg
     6541 cccccagaag atcatcgagt gcggcttgcc tgactggaac gcgagccgca gcggcctgcc
     6601 ctcgcccagc aggtgcgact ggccgatcac ctcggcgatc gtcttcgggc gcaggctttc
     6661 cgccagcggg gcggcggggt caagcttgaa aaggtcatcc atgggctgcg ttcttgttgt
     6721 ccatcttgcc tatgtatggc cggcgcagga aaaagcaaat gagaagcgca agtggcttac
     6781 tggttgacga cctcggcacc cttcggcacg tcgaacctga acgtctgcgc accaagcgcc
     6841 ggattacgct ggaagctcgt g
//
LOCUS       DASVLN010000968         1931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_373735, whole genome shotgun sequence.
ACCESSION   DASVLN010000968 DASVLN010000000
VERSION     DASVLN010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373735"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VF453_22775"
     CDS             complement(<1..1226)
                     /locus_tag="VF453_22775"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat-containing protein kinase
                     family protein"
                     /protein_id="HEX7690546.1"
                     /translation="MIAPHDPHATLADLRAGRLAGATRLDLRQAGLDEVPPEVLALGD
                     TLETLDVSGNRLEALPPGFERLHRLRVLFASDNRFAALPDVLGRCRELDICGFKANAI
                     AHVPAAALPASLRWLILTDNRVESLPDSLTACTRLQKLMLAGNRLARLPDPSGWQRLE
                     LVRLAANRFERRADALPGALLALPRLAWIAFGGNPFNAGDEARALAHAPVPDVAWDAL
                     SVGARLGEGASGIIHAATFDGAALAVKLFKGEVTSDGLPASEMAACVAAGSHPDIIGV
                     LGVVAGHPQEARGLVMRRIPAGYRNLAGPPSLASCTRDVYPDGPRLAAPVARAIAQGV
                     ADALAHLHARGVLHGDLYAHNLLVDATGHALLGDFGAASLLPGDDPALAEALVRLDRR
                     ALGVLVEELARRCDDPR"
     gene            1323..>1931
                     /locus_tag="VF453_22780"
     CDS             1323..>1931
                     /locus_tag="VF453_22780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7690547.1"
                     /translation="MAATAEDFIRESLEDPETFWTREAARIDWQRPFDRVCDTSRPPF
                     VQWFPGGLTNLCHNAVDRHAASTPDRDALVWVSTEVDLEKRWTCAELLREVQAMAAAL
                     QELGVRQGDRVLLYLPMIPEAAFAMLACARLGAIHSVVFGGFASASLAARIDDAKPTV
                     IVSADAGSRGGRVIAYKPLLDAAIEQAAHRPARVLLVDRGLAP"
BASE COUNT          253 a          693 c          758 g          227 t
ORIGIN      
        1 cgcgggtcgt cgcagcggcg cgccagttcc tcgaccagca ccccgagcgc ccgccggtcc
       61 agccgcacga gcgcctcggc cagggccgga tcgtcgccag gcagcagcga cgcggcgccg
      121 aagtcgccga gcagcgcatg cccggtggcg tcgacgagca ggttgtgcgc gtacaggtcg
      181 ccgtgcagga cgccgcgcgc gtgcaggtgg gcgagcgcat cggcgacgcc ttgcgcgatc
      241 gcccgggcga ccggggcggc gaggcgcggg ccgtcaggat agacgtcgcg ggtgcagctg
      301 gcgaggctgg gcgggccggc gaggttgcgg tagccggccg ggatgcggcg catcacgagt
      361 ccgcgtgcct cctgcggatg gccggcgacg acgccaagga caccgatgat gtccggatgc
      421 gagcccgcgg cgacgcaggc agccatctcg ctggcgggca ggccgtcgct cgtcacctcg
      481 cccttgaaca gcttgaccgc cagggccgcg ccgtcgaacg tcgccgcatg gatgatgccg
      541 gaggcaccct cgcccaggcg cgcgccgacg gacaacgcgt cccaggcgac gtccggcact
      601 ggcgcatggg cgagcgcccg cgcctcgtcg ccggcgttga aggggttgcc gccgaaggcg
      661 atccacgcga gccgcggcag cgcgagcagc gcgccgggca gcgcgtcggc gcggcgctcg
      721 aagcggttgg cggccaggcg cacgagctcc agccgctgcc atcccgacgg gtcgggcagg
      781 cgcgcgagcc ggttgccggc cagcatcagc ttctgcagcc gcgtgcacgc ggtgagcgag
      841 tcgggcagcg actcgacgcg gttgtcggtg aggatcagcc agcgcagcga ggccggcagc
      901 gccgccgccg gcacgtgcgc gatcgcgttg gccttgaagc cgcagatgtc gagctcgcgg
      961 caccggccga gcacgtccgg cagcgcggcg aagcggttgt ccgacgcgaa cagcacgcgc
     1021 agccggtgca ggcgctcgaa gcccggcggc agcgcctcca gtcggttgcc ggacacgtcg
     1081 agcgtctcca gggtgtcgcc gagcgccagc acctcgggcg gcacctcgtc gaggccggcc
     1141 tggcgcaggt cgaggcgggt cgcgccggcc aggcggccgg cgcgcaggtc ggcgagggtg
     1201 gcgtgcgggt cgtgcggtgc gatcatcggg cgggattgtc cgcgctccgc gccaagcctc
     1261 gcgtcaggcg gcgcgggcag gatgcggtca tcgcccaccc ggacaacgac gaaggagacg
     1321 ccatggcagc gaccgccgag gacttcatcc gcgagtccct cgaggatccc gagaccttct
     1381 ggacccgcga ggccgcgcgc atcgactggc agcggccgtt cgaccgcgtc tgcgacacct
     1441 cgcgcccgcc cttcgtgcag tggttcccgg gcggcctgac caacctctgc cacaacgccg
     1501 tcgaccgcca cgccgcgtcg acgccggacc gcgacgcgct ggtgtgggtc tcgaccgagg
     1561 tcgacctgga gaagcgctgg acctgcgccg agctgctgcg cgaggtgcag gcgatggcgg
     1621 cggcgctgca ggagctcggc gtgcgccagg gcgatcgcgt gctgctgtac ctgccgatga
     1681 tcccggaggc cgcgttcgcg atgctcgcct gcgcgcgcct gggggcgatc cactcggtgg
     1741 tgttcggcgg cttcgccagc gcgagcctcg ccgcgcgcat cgacgacgcg aagccgacgg
     1801 tgatcgtgag cgcggacgcg ggctcgcgcg gcggccgggt gatcgcgtac aagccgttgc
     1861 tggacgccgc catcgagcag gccgcgcacc ggcccgcgcg cgtgctgctg gtcgaccgcg
     1921 ggctggcgcc g
//
LOCUS       DASVLN010000969         5483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_481973, whole genome shotgun sequence.
ACCESSION   DASVLN010000969 DASVLN010000000
VERSION     DASVLN010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481973"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VF453_22785"
     CDS             complement(<1..275)
                     /locus_tag="VF453_22785"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEX7690548.1"
                     /translation="MRHASSRDGSLVLLFALAAAPLAAGAQVYKCRQADGSTTFQQVP
                     CPQARPAPAAARDPREQVAPPPSTDGRDDPYASDPCSRPQRAASLPPG"
     gene            634..2130
                     /locus_tag="VF453_22790"
     CDS             634..2130
                     /locus_tag="VF453_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX7690549.1"
                     /translation="MPSSTERASTGVAGLDAILHGGLIPRRIYLVDGNPGAGKTTLAQ
                     QFLLDGRRQGERCLYITLSETEEELRHGAESHGWSLDGIDILELAESDPDAHDDGELT
                     MFHPSEVELGQTMRRVLQAIERLEPQRLVFDSLSEMRLLAQGALRYRRQILALKQFFV
                     GRGCTVLLLDDRTSEGPDLQLQSIAHGVVSLEHVAPVYGRALRQLRVVKFRGSDFSSG
                     YHQMRVHRDGVEVFPRLSAADHGTPFEAGRLASGVAELDALLGGGIEIGSSTLVVGPA
                     GSGKSTLALQYACAAARRGHHARVFTFDESRQTMLARLRALGMMLEDGSGPGQVAVHQ
                     VDPAEITPGQFAHMVRDAVERDDARVIVIDSLNGYVNAMVDGHYLTAQLHELLSYLGN
                     LGVTSFLIAAQTGMVAGQMGTPGDASYLADCVIYLRFFEHGGQIKKAISVLKMRTGAH
                     EAAIRELRFDTTGIHLSPPLTQFRGILSGVPVELDDPSLLAGAIPSRR"
     gene            2127..3713
                     /locus_tag="VF453_22795"
     CDS             2127..3713
                     /locus_tag="VF453_22795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX7690550.1"
                     /translation="MTSTHEYSVAVLPATRRDGVVAAELLRRAGVDCRVVADAAALRR
                     ALEGDLGAVVLADTALAEPGFAAVTDALSRQPTWSEVPVILMSRAGEAGEQDGVAAVV
                     DRLNNVTVIDRPASIRTLVSAVQTALRARRRQYEIRDQIAALNEAQEALRQSDRRKDE
                     FLATLAHELRNPLAPLRSALQVMELRKPADPEQARLLAMMDRQMNVMVRLIDDLLDIA
                     RISSGKVTLRRQRLDLRKVIEAAAEAAMPAIAAAGHRLRVSLPEAPVHVLGDATRLAQ
                     VVGNLLNNATKYTGRGGDLSVTLDAAGGDATIVVRDNGAGIPPEVLKHVFDMFAQVNR
                     TLDRAQGGLGIGLSLVRQLVEMHGGSVDADSDGVDRGSTFTVRLPRLSEGDGDAIERR
                     GVRPEPSAAGPRLSVLVVDDNRDAADTLALLLAAAGHEVHTAYDGFQALASAGALRPG
                     VVFCDIGMPGMSGYEVAAMLRADAANAGTTLVAVTGWGNDIDKRLAHEAGFDFHLTKP
                     VHAGAIAGVLHRLPQRAAGD"
     gene            3718..4230
                     /locus_tag="VF453_22800"
     CDS             3718..4230
                     /locus_tag="VF453_22800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7690551.1"
                     /translation="MDESGAPGQADPRLPDIEELLALDLLTLRDHTERAGDRLEPAAH
                     RALLVGTQALSETIGVRRGGALAAYAMLSPQPDGCWFVSGFNIHPAHRDASVLRALFA
                     AIARLARRRAIASLRSHVYKTNARSLAFHRRLGFRVTRENAKAVEFTATLDELRGASR
                     AVRAARPGAD"
     gene            4284..>5483
                     /locus_tag="VF453_22805"
     CDS             4284..>5483
                     /locus_tag="VF453_22805"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HEX7690552.1"
                     /translation="MGLRGKLVALFFVGFALTCSVGIGLLIHNLGSDFAELEHREALR
                     LNDQLVRNFKAELEHLDELDTDWADWAGMYAFAQTLSPEFADAEVGTGALASAKLSFV
                     ALFDNRHRRVFLRAAADLVDQESNRGLARTLEAMERLFDAQGTRNTCSLHAADGEVYL
                     LCWQDIHLSDGSGPAHGTLLLGRLLDARILQRIRNQSGLDFAIERTFADTLPAGARTD
                     GRAVSTDDLSLGGADRHTLTGRMLDAAGQPVFLFHIRVPADIERSGRQITWRVVAVVA
                     VGALLGGLVLVLGTQVLLVRPLQRMERQLRDVGAAGGWPEQIAAAPGGDEIATLGRTI
                     NRMLRALREQGQALERQSLTDALTRLANRRAFDRALVAAVGHGRRSGAALSLLILDVD
                     HFKAYNDR"
BASE COUNT          735 a         2054 c         1968 g          726 t
ORIGIN      
        1 cccggcggca ggctggcggc ccgctgcgga cgcgagcacg gatcgctcgc gtagggatcg
       61 tcccggccgt ccgtcgacgg cggcggtgcg acctgctcgc gcggatcgcg cgccgccgca
      121 ggcgccggcc gcgcctgcgg acacggcacc tgctggaacg tcgtcgaacc gtcggcctgg
      181 cggcacttgt agacctgcgc cccggccgcc agtggcgccg cggcgagcgc gaacagcagg
      241 acgagcgagc cgtcccgcga ggatgcgtgt cgcattcgag tggtttcccc ctgctgcctt
      301 cgtggcgcat catcccacgg gcgaccggcc cggctggggg cctgcggccg cggccgcggc
      361 cgtgaaggat gcccgctcac tgccgtcctg cccgagcgca cgccgtttgc ccaggccgcg
      421 tgacggccct ggtcgcgcag cggcgtcgtc accgtggcgt gcggctgccg gctggacgcc
      481 cgtgcaggtg gcccggcagc cctggtccga tggccggccg gaacctcacg ccgcccatgc
      541 tgcgcagacg ccatggccgg gctgctcccg cgatacattc cggccagtgc tcgaccgccc
      601 cgacgaacca agatcttccg gagacttccg cccatgccca gctcgaccga acgcgcctcc
      661 acgggagttg ccggcctgga cgccatcctg cacggcggcc tgatcccccg gcggatctac
      721 ctcgtcgacg gcaacccggg cgcgggcaag accacgctcg cgcagcagtt cctgctcgac
      781 ggccggcgcc agggcgagcg ctgcctctac atcacgctgt cggagaccga ggaagagttg
      841 cggcacgggg ccgagtcgca cggctggtcg ctcgacggca tcgacatcct cgaactcgcg
      901 gagtccgacc cggacgccca cgacgacggc gagctcacga tgttccatcc ctcggaagtc
      961 gagctcggcc agacgatgcg gcgcgtgctg caggcgatcg agcggctcga gccgcagcgg
     1021 ctggtgttcg actcgctgtc cgagatgcgc ctgctcgcgc agggcgcgct gcgctaccgg
     1081 cgccagatcc tcgcgctcaa gcagttcttc gtcggccgcg gctgcacggt gctgctgctc
     1141 gacgaccgca cctccgaggg ccccgacctg cagctgcaga gcatcgccca cggcgtggtg
     1201 tcgctggaac acgtcgcacc ggtctacggc cgggccctgc gccagctgcg cgtcgtgaag
     1261 ttccgcggca gcgacttcag cagcggctat caccagatgc gcgtgcaccg ggacggggtc
     1321 gaggtcttcc cgcggctgtc ggcggcagac cacggcaccc cgttcgaggc cggccggctc
     1381 gcgagcggcg tcgcggagct ggacgccctg ctcgggggcg gcatcgagat cggctccagc
     1441 acgctcgtcg tcgggccggc gggctcgggg aagtcgaccc tcgcgctgca gtacgcgtgc
     1501 gccgccgcgc gccgcggcca ccatgcgcgc gtgttcacct tcgacgagtc gcggcagacc
     1561 atgctcgcgc ggctgcgggc gctcggcatg atgctggagg acggctcggg acccggccag
     1621 gtggcggtac accaggtcga cccggccgag atcacgccag ggcagttcgc gcacatggtt
     1681 cgcgacgccg tggagcgcga cgacgcgcgc gtgatcgtga tcgacagcct gaacggctac
     1741 gtcaacgcga tggtggacgg ccactacctg accgcgcagc tgcacgagct cctgtcctac
     1801 ctcggcaacc tgggcgtcac ctcgttcctc atcgccgcgc agaccggcat ggtcgccggc
     1861 cagatgggga cgccgggcga cgccagctac ctggccgact gcgtgatcta cctgcgcttc
     1921 ttcgagcacg gcggccagat caagaaggcg atctcggtgc tgaagatgcg caccggggcg
     1981 cacgaggcgg ccatccgcga gctgcgcttc gacacgaccg gcatccactt gagcccgccg
     2041 ctgacgcagt tccgcggcat cctctcgggc gtgcccgtcg agctcgacga cccgtcgctc
     2101 cttgccggcg cgatcccctc acgtcgatga ccagcaccca cgagtacagc gtcgccgtgc
     2161 tgccggccac gcgccgcgac ggcgtcgtcg ccgccgagct gctgcgccgc gccggcgtcg
     2221 actgccgggt ggtggccgat gcggcggccc tgcggcgggc cctcgagggc gacctcggcg
     2281 ccgtcgtcct ggccgacacg gcgctggccg aaccgggatt cgccgccgtc accgatgcgc
     2341 tctcccgcca gccgacctgg tcggaggtgc ccgtgatcct catgagccgg gccggcgagg
     2401 ccggggagca ggacggcgtc gccgcggtcg tcgaccggct gaacaacgtc accgtgatcg
     2461 accgcccggc ctcgatccgc acgctcgtca gcgccgtcca gaccgcgctg cgcgcgcggc
     2521 ggcgccagta cgagatccgc gaccagatcg cggcgctgaa cgaggcccag gaggcgctgc
     2581 ggcagtccga ccggcgcaag gacgaattcc tcgcgacgct cgcccacgag ctgcgcaacc
     2641 cgctcgcccc gctgcgctcg gcgctgcagg tgatggagct gcgcaagccg gcggaccccg
     2701 agcaggcccg gctgctggcc atgatggacc ggcagatgaa cgtgatggtg cggctgatcg
     2761 acgacctgct cgacatcgcg cgcatctcca gcggcaaggt gacgctgcgc cgccagcggc
     2821 tcgacctgcg caaggtgatc gaggccgcgg ccgaggcggc gatgccggcg atcgccgccg
     2881 ccggccatcg cctgcgagtc tcgctgccgg aggcgccggt ccatgtgctg ggcgacgcga
     2941 cccggctggc gcaggtggtc ggcaacctgt tgaacaacgc gacgaagtac acgggccgcg
     3001 gcggcgacct ctcggtgacg ctcgacgccg ccggcggcga cgcgacgatc gtcgtgcgcg
     3061 acaacggcgc cggcatcccg ccggaggtcc tcaagcacgt gttcgacatg ttcgcgcagg
     3121 tcaaccgcac gctcgaccgc gcgcaaggcg ggctcgggat cggcctgtcg ctcgtgcgcc
     3181 agctcgtgga gatgcacggg ggcagcgtcg atgccgacag cgacggcgtc gaccgcggca
     3241 gcacgttcac ggtgcggctg ccgcggctgt ccgagggcga cggcgatgcc atcgagcggc
     3301 gcggcgtgcg gccggagccg tccgcggcgg ggccgcgcct gagcgtgctc gtcgtcgacg
     3361 acaaccgcga cgcggccgac acgctcgcgc tcctgctcgc ggccgctggc cacgaggtgc
     3421 ataccgccta tgacggcttc caggcgctcg cctcggcggg tgcgctgcgg cccggcgtcg
     3481 tcttctgcga catcggcatg ccggggatga gcggctacga ggtcgccgcc atgctgcgcg
     3541 ccgacgcggc caacgccggc accacgctgg tcgccgtcac cggctggggc aacgacatcg
     3601 acaagcgcct cgcccacgag gccggcttcg atttccatct gaccaagccc gtccacgccg
     3661 gcgcgatcgc gggcgtgctg caccggctgc cgcagcgggc ggccggggac tgaacgcatg
     3721 gacgaatccg gcgcgcccgg gcaggccgat ccccgcctgc ccgacatcga ggaactgctc
     3781 gccctcgacc ttctcaccct gcgcgaccac acggaacgcg ccggcgaccg cctcgagccg
     3841 gcggcccacc gggcgctgct cgtgggcacg caggcgctgt ccgagacgat cggcgtgcgc
     3901 cgcggcggcg cgctcgcggc gtacgcgatg ctgagcccgc agccggacgg ctgctggttc
     3961 gtctccggct tcaacatcca tcccgcgcat cgcgacgcga gcgtcctgcg cgcgctcttc
     4021 gcggcgatcg cgcggcttgc acggcggcgc gccatcgcgt cgctgcgcag ccacgtctac
     4081 aagacgaacg cgcgctccct cgcgttccac cggaggctcg gcttccgcgt cacgcgcgag
     4141 aacgcgaagg ccgtcgagtt cacggcgacg ctcgacgaac ttcgcggcgc cagccgcgcc
     4201 gtccgcgcgg cccgcccggg tgcggactga gtccggccct ggcgcggccg cccaggcacg
     4261 gcgggtgccc gcgacgcagg gccatgggac tccgaggaaa gctggtcgcg ctgttcttcg
     4321 tcggcttcgc attgacgtgc agcgtgggca tcggcctgct gatccacaac ctcggctccg
     4381 acttcgccga gctggagcat cgcgaggcgc tgcggctgaa cgaccagctc gtgcgcaact
     4441 tcaaggcgga gctcgagcac ctggacgagc tcgacaccga ctgggcggac tgggccggca
     4501 tgtacgcgtt cgcgcagaca ctgtcgcccg agttcgccga cgccgaggtc ggcacgggcg
     4561 cgctcgcgag cgcgaagctg agcttcgtcg ccctcttcga caaccgtcac cgacgcgtct
     4621 tcctgcgcgc cgcggcggat ctcgtcgacc aggagtccaa ccgcggcctc gcacgcacgc
     4681 tggaggcgat ggagcgcctg ttcgatgccc agggcacgcg caacacgtgc agcctgcatg
     4741 ccgccgacgg cgaggtctac ctgctgtgct ggcaggacat ccacctcagc gacggatcgg
     4801 gcccggcgca cggcacgctg ctgctcgggc ggctgctgga cgcgcggatc ctgcagcgga
     4861 tccgcaacca gagcggcctc gacttcgcga tcgagcggac gttcgcggac accctgccgg
     4921 ccggcgcccg cacggacggc cgcgcggtct cgaccgacga cctctcgctc ggcggcgccg
     4981 accgccacac gctgacgggc cggatgctcg acgccgcggg ccagccggtc ttcctgttcc
     5041 acatccgcgt gcccgccgac atcgagcgca gcggtcggca gatcacgtgg cgggtggtgg
     5101 cggtggtcgc ggtgggcgcg ctgctgggcg gcctggtgct ggtgctgggc acgcaggtgc
     5161 tgctggtgcg gcccctgcag cgcatggagc gccagctgcg cgacgtcggc gccgcgggcg
     5221 ggtggccgga gcagatcgcg gccgcgccgg gcggcgacga gatcgcgacc ctcggccgga
     5281 cgatcaaccg catgcttcgg gcgctgcgcg agcaggggca ggcgctcgag cggcagtcgc
     5341 tgaccgacgc gctgacccgc ctggccaacc ggcgcgcctt cgaccgcgcc ctggtcgcgg
     5401 ccgtcggcca cggccggcgc agcggcgcgg cgctgtcgct gctgatcctc gacgtcgacc
     5461 acttcaaggc ctacaacgac cgc
//
LOCUS       DASVLN010000970         4487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_48847, whole genome shotgun sequence.
ACCESSION   DASVLN010000970 DASVLN010000000
VERSION     DASVLN010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48847"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..3322)
                     /locus_tag="VF453_22810"
     CDS             complement(179..3322)
                     /locus_tag="VF453_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7690553.1"
                     /translation="MSKFFIDRPIFALVIALFILVGGSVAITQLPIAQYPPVAPPTIV
                     LNATYPGASAQTMDEAVISVIEQEMNGSPGLIYMESVSQANGTGSITISYQPGTNPDL
                     AQVDVQNRLSRAAPRLPAAVTQQGVRVDKARSNFLLFTILSSDNPAYDPVALGDYASR
                     NVLPEIQRIPGVGQAQLFGTERAMRIWVDPQKLVGFKLSMAQVDAAIAAQNAQVAAGA
                     VGDLPNLTQQGITATVVVQGQLTNVDQFGAIVLRANPDGSSVRLRDVARIELGAQTYA
                     TSARLNGHPSTGIGVQLSPTGNALQTAQAIKDKMAQLQRYFPQGVKYSIPYDSSRFIK
                     ISISQVVETLLEAVALVFVVMFVFLQNWRYTIIPTIVVPVALLGTFAVLLAMGYSINV
                     LTMFGMVLVIGIVVDDAIVVVENVERIMSEEGLPPVEATKKAMSQIQGAIVGVTVVLI
                     SVFVPLAFFAGSVGNIYRQFSTVMVASIALSAFMALSLTPALCGVLLRQVQAGHHDEK
                     RGFFGWFNRSFNRTAKRYENGVRHVLHRTGRMMFAYLAVLAAVALFFWKLPTSFLPGE
                     DQGYLIVNVQLPPGATRNRALEVVKQIEGYVLKQPEVQSMVGVLGFSFAGSGQNAALA
                     FVTLKDWDQRRGQEHSAQAIAGRAMGALSRVRDAFIFALSPPPIPELGTASGWAFRLQ
                     DRAGAGHAALLAARNQLLGAAMKDPILAGVRPDGLEDASQLRLTIDREKAASLGVTFD
                     AINSAISTGFGSAYVNDFPNAGRLQRVVVQADAAERMQAEDLLRLNALNSSGQPVPLS
                     AFATAEWITGPVQSIRYNGYPAMRIAGDASPGHSTGEAMAEMERLAAQLPQGFGFEWT
                     GQSREEKLSGSTAVILLGFSLLAVFLCLAALYESWSIPAAVLLVVPVGVLGALLGAWS
                     RGFSNDVYFKVGLITIIGLSAKNAVLIIEFAKDLQAQGKSATDAALEAVRLRFRPIIM
                     TSLAFMLGVLPLAISRGAGSASQRAIGTGVMGGMATATLVGVLFVPLFFVLVRRVFKG
                     SRRQHEFDVAHSRSFKH"
     gene            complement(3348..>4487)
                     /locus_tag="VF453_22815"
     CDS             complement(3348..>4487)
                     /locus_tag="VF453_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7690554.1"
                     /translation="AALAFALAGCDKPAPPPQMPPPEVGVVTVAPRVVAVPTELPGRI
                     EASRVAQVRARVTGIVLKRLFVEGSQVKAGQTLFLIDPAPYKAALDSAKAALAKAQAN
                     AQQAAQLVARYKPLREANAISQQDFVNATSAAAQGEADVAAARAAVQTAQLNLDYATV
                     TAPISGRIGQALVTEGALVSASEATQLALIQQIDPVYANFTQPAADITRLRRAFASGK
                     AKSTGSSIPVHLTLDDGSPAPQAGRLLFSDLTVDPTSGQVVVRAEVPNPDGTLLPGMY
                     VRGRLAQSEVDGAVLLPQQAVNRTPQGDVAMVVDAQGVVSPRPLKLGGVDGGDWIVLS
                     GLKAGEQVMVDGFQKLRPGVTVKPVPWSAASAPAAAAPAASAASR"
BASE COUNT          666 a         1574 c         1632 g          615 t
ORIGIN      
        1 cggcgaggac gcgcccgggg cggccgcgta gggaaaggcc gcggccacgg gcgcggcggg
       61 gcgctcgtag cgcggcgcca tgctggaaca ggccgccagc gaacccgcgg ccagcgcgag
      121 cacgccgcgg cgcgcgaagg aacgaacgct catcggggat ctcgtcgtgg tcacgtcgtc
      181 aatgcttgaa ggagcgcgag tgcgcgacgt cgaactcgtg ctggcgccgg ctgcccttga
      241 agacgcggcg caccagcacg aagaacagcg gcacgaacag cacgcccacc agcgtcgcgg
      301 tggccatgcc gcccatcacg ccggtgccga tcgcacgctg gctcgcggag cccgcgccgc
      361 gcgagatcgc cagcggcagc acgcccagca tgaacgcgag cgaggtcatg atgatcgggc
      421 ggaagcgcag ccgcactgcc tcgagcgcgg catcggtggc gctcttgccc tgcgcctgca
      481 ggtccttcgc gaactcgatg atcagcaccg cgttcttcgc cgacaggccg atgatggtga
      541 tcaggccgac cttgaagtac acgtcgttgg agaagccgcg cgaccaggcg cccagcagcg
      601 cgccgagcac gcccaccggc acgaccagca gcacggcggc cgggatggac cagctctcgt
      661 acagcgccgc caggcacagg aacaccgcca gcagcgagaa cccgagcagg atcaccgccg
      721 tcgagccgga gagcttctcc tcgcgcgact ggcccgtcca ctcgaaaccg aagccctgcg
      781 gcagctgcgc cgcgaggcgc tccatctcgg ccatcgcctc gccggtcgaa tggccgggcg
      841 aggcgtcgcc cgcgatgcgc atcgccgggt agccgttgta gcggatgctc tgcaccgggc
      901 cggtgatcca ttcggcggtc gcgaaggcgg acagcggcac cggctggccc gaggagttca
      961 gcgcgttcag gcgcagcagg tcctcggcct gcatgcgctc ggccgcgtcg gcctgcacga
     1021 cgacgcgctg caggcggccg gcgttcggga agtcgttgac gtaggccgag ccgaagccgg
     1081 tcgagatcgc cgagttgatg gcgtcgaagg tcacgcccag gctcgccgcc ttctcgcggt
     1141 cgatcgtcag gcgcagctgg gaggcgtcct ccaggccgtc gggccgcacg ccggcgagga
     1201 tcggatcctt catcgccgcg cccagcagct ggttgcgcgc ggccagcagc gccgcgtggc
     1261 cggcgccggc gcggtcctgc aggcggaacg cccagccgga ggcggtgccc agctcgggga
     1321 tcggcggcgg gctgagcgcg aagatgaacg cgtcgcgcac gcgcgagagc gcgcccatcg
     1381 cgcggccggc gatggcctgg gccgagtgct cctggccgcg gcgctggtcc cagtccttga
     1441 gggtgacgaa cgcgagcgcc gcgttctggc ccgagcccgc gaagctgaag cccagcacgc
     1501 ccaccatgct ctgcacctcg ggctgcttga gcacgtagcc ctcgatctgc ttgacgacct
     1561 ccagcgcgcg gttgcgcgtg gcacccggcg gcagctgcac gttgacgatc aggtagccct
     1621 ggtcctcgcc cgggaggaac gaggtcggca gcttccagaa gaacagcgcc acggcggcca
     1681 gcaccgcgag gtaggcgaac atcatgcggc cggtgcggtg caggacatgg cgcacgccgt
     1741 tctcgtagcg cttggcggtg cggttgaagc tgcggttgaa ccagccgaag aagccgcgct
     1801 tctcgtcgtg gtggccggcc tgcacctggc gcagcagcac gccgcacagc gcgggcgtca
     1861 gcgacagcgc catgaacgcc gacagcgcga tcgaggccac catcaccgtc gagaactggc
     1921 ggtagatgtt gcccaccgag ccggcgaaga acgcgagcgg cacgaacacc gagatcagca
     1981 ccaccgtcac gccgacgatc gcgccctgga tctggctcat cgccttcttg gtcgcctcga
     2041 ccggcggcag gccctcctcg ctcatgatgc gctcgacgtt ctcgaccacc acgatcgcgt
     2101 cgtcgaccac gatgccgatc acgagcacca tgccgaacat cgtcagcacg ttgatcgagt
     2161 agcccatcgc cagcagcacc gcgaaggtgc ccagcagcgc gaccggcacg acgatggtcg
     2221 ggatgatcgt gtagcgccag ttctgcagga acacgaacat gacgacgaac acgagcgcca
     2281 cggcctcgag cagcgtctcg accacctgcg agatcgagat cttgatgaag cgcgagctgt
     2341 cgtaggggat gctgtacttc acgccttgcg ggaagtagcg ctgcagctgg gccatcttgt
     2401 ccttgatcgc ctgcgcggtc tgcagcgcgt tgccggtggg cgagagctgc acgccgatgc
     2461 cggtcgaggg atggccgttc aggcgcgccg aggtcgcgta ggtctgcgcg ccgagctcga
     2521 tgcgcgcgac gtcgcgcagg cgcacgctgg agccgtcggg attggcgcgc agcacgatcg
     2581 cgccgaactg gtcgacgttg gtcagctggc cctgcacgac caccgtcgcg gtgatgccct
     2641 gctgcgtcag gttgggcagg tcgcccacgg cgccggcggc gacctgggcg ttctgcgccg
     2701 cgatcgccgc gtcgacctgc gccatcgaca gcttgaagcc gaccagcttc tgcgggtcga
     2761 cccagatgcg catcgcgcgc tcggtgccga acagctgcgc ctggccgacg ccggggatgc
     2821 gctggatctc cggcagcacg ttgcgcgagg cgtagtcgcc gagcgcgacc gggtcgtagg
     2881 ccgggttgtc ggaggacagg atcgtgaaca gcaggaagtt cgagcgcgcc ttgtcgacgc
     2941 gcacgccctg ctgcgtcacc gccgcgggca ggcggggcgc cgcgcgcgac aggcggttct
     3001 gcacgtcgac ctgcgcgagg tcgggattgg tgccgggctg gtagctgatc gtgatcgagc
     3061 cggtgccgtt ggcctggctg accgactcca tgtagatgag gcccggcgag ccgttcatct
     3121 cctgctcgat gacggagatc accgcctcgt ccatcgtctg cgccgaggcg ccgggatagg
     3181 tggcgttcag gacgatggtc ggcggcgcga ccggcgggta ctgggcgatc ggcagctgcg
     3241 tgatcgccac gctgccgccc acgaggatga acagggcgat gaccagcgcg aagatcgggc
     3301 ggtcgatgaa gaacttggac atgggggagc gcgcctcggg ttcttcttca gcggctggcc
     3361 gcgctcgccg ccggcgccgc ggcggccggc gcggatgcgg cgctccacgg caccggcttg
     3421 accgtcacgc cgggccgcag cttctggaag ccgtcgacca tcacctgctc gcccgccttc
     3481 aggccggaca gcacgatcca gtcgccgccg tcgacgccgc ccagcttgag cggccgcggc
     3541 gagaccacgc cctgcgcgtc caccaccatc gcgacgtcgc cctgcggcgt gcggttgacg
     3601 gcctgctgcg gcagcagcac cgcgccgtcg acctccgact gcgcgagccg gccgcgcacg
     3661 tacatgccgg gcagcagcgt gccgtccggg ttgggcacct cggcgcgcac cacgacctgg
     3721 ccggaggtcg gatccacggt caggtcggag aacagcagcc tgccggcctg cggcgccggg
     3781 ctgccgtcgt ccagcgtcag gtgcacggga atcgagctgc cggtcgactt cgccttgccc
     3841 gacgcgaacg cgcggcgcag gcgggtgatg tcggcggcgg gctgcgtgaa gttggcgtag
     3901 acggggtcga tctgctggat cagcgcgagc tgcgtggcct cgctggcgct caccagcgcg
     3961 ccctccgtga ccagcgcctg gccgatgcgg ccgctgatcg gcgcggtgac ggtcgcgtag
     4021 tcgaggttga gctgcgcggt ctgcacggcc gccctggccg ccgcgacgtc ggcctcgccc
     4081 tgcgcggcgg ccgaggtcgc gttgacgaag tcctgctggc tgatcgcgtt ggcctcgcgc
     4141 agcggcttgt agcgcgcgac gagctgcgcc gcctgctgcg cgttggcctg ggccttggcg
     4201 agcgcggcct tcgcgctgtc gagcgcggcc ttgtacggcg ccgggtcgat caggaagagc
     4261 gtctgcccgg ccttgacctg gctgccctcg acgaacaggc gcttgagcac gatgcccgtc
     4321 acccgcgcgc gcacctgcgc cacgcgcgag gcctcgatgc ggccgggcag ctcggtcggc
     4381 accgcgacca cccgcggcgc gaccgtcacc acgccgactt ccggcggcgg catctgcggc
     4441 ggcggcgccg gcttgtcgca gccggcgagc gcgaacgcga gcgcggc
//
LOCUS       DASVLN010000971         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_266536, whole genome shotgun sequence.
ACCESSION   DASVLN010000971 DASVLN010000000
VERSION     DASVLN010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266536"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..737)
                     /locus_tag="VF453_22820"
     CDS             complement(150..737)
                     /locus_tag="VF453_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEX7690555.1"
                     /translation="MKLQAILKAGLAAVALAAAAGCSSTASVTGAERPGASTAEGRKS
                     IDAGYHETLERLYATTPGSRELVAKARGVLIFPRVIAAGLVVGGAYGEGELRTGGAIE
                     GYYRTTTGSVGWQIGAQSRALVFLFMTQDALDRFRASHGWSGGVDASVAVLKVGANGA
                     IDANAEHAPTVAFVMTNAGLMADLTLQGTKVTRID"
     gene            921..>2822
                     /locus_tag="VF453_22825"
     CDS             921..>2822
                     /locus_tag="VF453_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7690556.1"
                     /translation="MQASGETSTARSAAPRPAAQPWASAGLLWGGVVVLVAIAAFLAF
                     SLKASHDRYQANAASQLENLTLNLDRYFFARLQSADLVLQAAVREYEQDRGDPRKAAD
                     FEALLAQLQQELPGAPAIRAVDAQGVVRFGPGSDGPRALNIANRRFFREAMETPGLVV
                     GLPLKSRLTERWIVPLARRLPGVDGKPGGVVYLPLELEDVARVVAPLNIGEHGVIVLF
                     TPRMEVLLRRPALPLLGDERPVQFQAPEMRAAFAAGKLATLISARSTIDQRVRTTMYR
                     QIESYPVYVLVGFSDDDVFAPWWREVALAVVVWCALAASAVLLLSALRRASRQQALAL
                     EEQRLLTERADAASRAKSAFLANMSHEIRTPMNAIIGLTHLMARDVRDDVQKDRLAKV
                     DDAARHLLQVINDILDLSKIEAGKMVLEQAEFSLDLLLGRALEMIANQAHAKGLELVL
                     DSTRVPDLLRGDPTRLSQALINLLSNAVKFTERGWIRLGVEIVGHSPGGIELRFEVQD
                     TGPGISASQQAALFSAFEQADTTTTRRFGGTGLGLALTRHLAQMMGGTAGLSSVVGQG
                     SRFWFTARFERPAQREERASDLPAGLRALLVDDLPQARDALGEQLRRLGFEVDACADG
                     HAALDRATRE"
BASE COUNT          358 a         1031 c         1050 g          383 t
ORIGIN      
        1 cgcgctcgcc gcacgcggcc tgccgggcga gcgcatcttc atggccgcgc ctcggctgca
       61 gggccgcgac ggcgccgcgc ggccgccgga ctggcagccg cacgtggtgc tcgcgctgac
      121 cacgaggtga gtcggcgcgg cgcccggcgt cagtcgatgc gcgtgacctt cgtgccctgc
      181 agcgtgaggt cggccatcag gccggcgttg gtcatcacga aggcgacggt cggggcgtgt
      241 tcggcgttcg cgtcgatcgc gccgttggcg ccgaccttca gcacggcgac ggaggcgtcg
      301 acgccgccgc tccagccgtg gctcgcgcgg aagcggtcga gcgcatcctg cgtcatgaac
      361 aggaacacga gcgcccgcga ctgcgcgccg atctgccagc ccaccgagcc ggtggtcgtg
      421 cggtagtagc cctcgatcgc gccgccggtg cgcagctcgc cctcgccgta cgcgccgccc
      481 accaccaggc cggccgcgat cacgcgcggg aagatgagca cgccgcgcgc cttggccacg
      541 agctcgcggc tgcccggcgt cgtcgcgtac aggcgctcga gggtctcgtg gtagccggcg
      601 tcgatgctct tgcggccctc cgcggtcgag gcgcccgggc gctcggcgcc ggtcacgctg
      661 gcggtggacg agcagcccgc cgcggcggcg agggcgacgg cggcgagtcc tgccttgagg
      721 atggcttgca gtttcatggg ttctccttgc gaggccggcg gcgccggcgt gtcgcgccgc
      781 aggcagcaat cccggtgccc ggccgggacg cgggcatgtc cgctgccgtg cgcttgacct
      841 gccgccgccg atcgggggca gaatcgcggc tggccggcag cgcacggccc tcgacgaaca
      901 aacgccgatc cgggaggacg atgcaagcga gcggtgagac ctcgaccgcg cggtccgccg
      961 cgccgcggcc ggccgcgcag ccctgggcgt ccgccgggct gctctggggc ggcgtggtcg
     1021 tcctcgtcgc gatcgcggcc ttcctcgcgt tctcgctcaa ggcgagccac gaccgctacc
     1081 aggccaacgc ggcgagccag ctcgagaacc tgacgctcaa cctggaccgc tacttcttcg
     1141 cgcgcctgca gtcggccgac ctggtgctgc aggccgcggt gcgggaatac gagcaggatc
     1201 gcggcgaccc gcgcaaggcc gccgacttcg aggcgctgct cgcccagctg cagcaggagc
     1261 tgcccggcgc gcccgcgatc cgcgccgtcg acgcgcaggg cgtggtgcgc ttcggccccg
     1321 gcagcgacgg cccccgcgcg ctgaacatcg ccaaccggcg cttcttccgc gaggcgatgg
     1381 agacgccggg gctggtggtc gggcttccgc tgaagtcgcg cctcaccgag cgctggatcg
     1441 tgccgctcgc gcgccggctg cccggcgtcg acggcaagcc gggcggcgtg gtctacctgc
     1501 cgctggagct ggaagacgtc gcgcgcgtgg tggcgccgct gaacatcggc gagcacggcg
     1561 tcatcgtgct gttcacgccg cgcatggagg tgctgctgcg ccgccccgca ctgccgctgc
     1621 tgggcgacga gcgaccggtg cagttccagg cgcccgagat gcgcgcggcc ttcgccgctg
     1681 gcaagctcgc cacgctgatc tcggcgcgtt cgacgatcga ccagcgcgtg cgcacgacga
     1741 tgtaccgcca gatcgagtcc tacccggtct acgtgctggt gggcttctcc gacgacgacg
     1801 tcttcgcgcc gtggtggcgg gaggtggcgc tcgcggtggt ggtgtggtgc gcgctcgcgg
     1861 cgagcgccgt gctgctgctg tccgcgctgc ggcgcgcgtc gcggcagcag gcgctcgcgc
     1921 tcgaggagca gcgcctgctc accgagcgcg ccgacgccgc gagccgcgcg aagagcgcct
     1981 tcctcgccaa catgagccac gagatccgca cgccgatgaa cgccatcatc ggcctcacgc
     2041 acctgatggc ccgcgacgtg cgcgacgacg tgcagaagga ccgcctcgcc aaggtcgacg
     2101 acgcggcccg ccacctgctg caggtgatca acgacatcct cgacctgtcc aagatcgagg
     2161 ccggcaagat ggtgctcgag caggccgagt tctcgctgga cctgctgctc gggcgcgcgc
     2221 tcgagatgat cgccaaccag gcgcacgcca agggcctgga gctggtgctc gacagcacgc
     2281 gcgtccccga cctgctgcgc ggcgacccga cgcggctgtc gcaggcgctg atcaacctgc
     2341 tgtccaacgc cgtcaagttc accgagcgcg gctggatccg cctgggcgtc gagatcgtcg
     2401 gccattcgcc cggcggcatc gagctgcgct tcgaggtcca ggatacaggg cccggtatct
     2461 cggcatcgca gcaggcggcg ctgttctcgg cgttcgagca ggccgacacg acgacgacgc
     2521 gccgcttcgg cggcacgggg ctgggcctcg cgctgacgcg ccacctcgcg cagatgatgg
     2581 gcgggacggc ggggctgtcc agcgtcgtcg ggcagggcag ccggttctgg ttcacggcca
     2641 ggttcgagcg ccctgcgcag cgcgaggaac gcgcgagcga cctgccagcg ggcctgcggg
     2701 cgctgctggt cgacgacctg ccgcaggcgc gcgacgcgct cggcgagcag ctgcgccggc
     2761 tcggcttcga ggtcgacgcc tgcgccgacg ggcacgcggc gctcgaccgc gcgacgcgcg
     2821 ag
//
LOCUS       DASVLN010000972         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_427888, whole genome shotgun sequence.
ACCESSION   DASVLN010000972 DASVLN010000000
VERSION     DASVLN010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427888"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VF453_22830"
     CDS             <1..213
                     /locus_tag="VF453_22830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690557.1"
                     /translation="GVQGEGSLRHVMWRPIERAAWEPPVAGDPGLEWVEADDLETQRR
                     LLAEGSIGVRMRDGKAAIGFARPPGP"
     gene            254..1945
                     /locus_tag="VF453_22835"
     CDS             254..1945
                     /locus_tag="VF453_22835"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEX7690558.1"
                     /translation="MRAGRIRSASRKVRASPGRGSWPGHRCCLSSPGPGRQRGAPGCR
                     GGPAPAASTTKPRGTRTHHRHPVALAALAVLATLAGAARAGGFSAGNLIVSRTVYAGD
                     ASTVAVGQALPGGGKAIADGSFPNVFKNESPDPSFGITAPVFLDQLTQSGAHVSTLAI
                     DPARLTTSFASKSELGLNISQDGRSVTFMGYVAAVNQLDVSNANTPGVLDPTNPVKTV
                     VQRGIATVSLATGALGVTAVNAYSGNNGRNAVLAGGNYYTVGNAGNSGKGVSGAVLSQ
                     LSDDTGLQMIAAGQSGATTVVGKTYGSAGSTTGYQHGFSTVATGGAADKTGKDDNFRG
                     ETLFGNTLYVTKGSGGNGVNTVYQVGTAGELPSATTASSIAITPLAGFPTSGTATHPF
                     GLWFANSTTLFVADEGDGQARGTPGKDMSGAGLDEYRLVGGSWVETQVFQKGLLDQAG
                     YSEGLLWTVQTDGLLNLTGEVDADGSYTLFATTSTVSNESTHDQGADPNEIVSIHLDA
                     ASTAADTSFSIVETAAAGERFGGVALTPVPEPGNAALLLAGVGLLAGAARRRRVG"
     gene            2087..2539
                     /locus_tag="VF453_22840"
     CDS             2087..2539
                     /locus_tag="VF453_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7690559.1"
                     /translation="MYARILVPIDGSPTAECGLGEAIRLAQAMRGRIVALTVSAGFVG
                     AMEMASARDYQESSARQLKACTDLVDAAASRARDAGVECDKVVADNRGRSVAQTILDE
                     CATWNCDLVVMGTHGRRGLTRMVLGSDAEAVLRGARTPVMLVRPSEAH"
BASE COUNT          367 a          975 c          929 g          330 t
ORIGIN      
        1 ggggtgcagg gcgagggctc gctgcggcac gtgatgtggc ggccgatcga gcgcgccgcc
       61 tgggagccgc cggtggccgg cgacccgggg ctcgaatggg tggaggccga cgacctcgag
      121 acgcagcggc gcctgctggc cgagggcagc atcggcgtgc gcatgcgcga cggcaaggcg
      181 gcgatcggat tcgcgcggcc cccgggcccg tagccgcccg cggccgggcg tggcatcgcg
      241 cggctcttgc gcgttgcgtg cgggccgcat tcgttccgct tcgcgcaagg ttcgggcctc
      301 gcctggacgc gggtcatggc ccggacatcg atgctgccta agttcccctg gccccggccg
      361 ccagcgcggc gcgccggggt gccgcggcgg acctgctccc gcggcctcca cgacgaaacc
      421 cagaggaact cgcacgcacc atcgccatcc cgtcgccctt gcggcgctcg ccgtcctcgc
      481 gacgctcgcc ggcgccgccc gcgccggcgg cttttccgcc ggcaacctga tcgtctcgcg
      541 cacggtctac gccggcgacg cgtccaccgt cgccgtcggc caggcgctgc ccggcggcgg
      601 caaggccatc gccgacggct cgttccccaa cgtcttcaag aacgagtcgc ccgatccctc
      661 gttcggcatc accgcgccgg tcttcctcga ccagctcacg cagtcgggcg cgcacgtgag
      721 cacgctcgcg atcgatcccg cgcggctgac gacgagcttc gcgtcgaaat ccgagctggg
      781 cctgaacatc tcgcaggacg gcaggtcggt caccttcatg ggctatgtcg ccgccgtgaa
      841 ccagctggac gtctccaacg ccaacacgcc cggcgtcctg gacccgacca atccggtcaa
      901 gacggtcgtc cagcggggca tcgccaccgt cagcctcgcc accggggcgc tcggcgtgac
      961 cgcggtcaac gcctacagcg gcaacaacgg ccgcaacgcg gtgctggccg gcggcaacta
     1021 ctacaccgtc ggcaacgccg gcaactcggg caagggcgtc agcggcgcgg tgctgtccca
     1081 gctgagcgac gacaccggcc tgcagatgat cgcggcgggc cagtcgggcg cgaccacggt
     1141 cgtcggcaag acctacggca gcgccggcag cacgaccggc taccagcacg gcttctccac
     1201 cgtcgccacc ggcggcgcgg ccgacaagac cggcaaggac gacaacttcc gcggcgagac
     1261 cctgttcggc aacacgctgt acgtgaccaa gggcagcggc ggcaacggcg tcaacacggt
     1321 ctaccaggtc ggcacggccg gcgagctgcc gagcgccacg accgcgtcgt cgatcgccat
     1381 cacgccgctc gccggcttcc cgacgtccgg caccgccacg catccgttcg gcctgtggtt
     1441 cgcgaattcg accaccctgt tcgtggccga cgagggcgac ggccaggcgc gcggcacgcc
     1501 gggcaaggac atgtccggcg ccggcctcga cgaataccgc ctcgtcggcg gcagctgggt
     1561 ggagacgcag gtcttccaga agggcctgct cgaccaggcc ggctacagcg agggcctgct
     1621 ctggacggtg cagaccgacg gcctgctcaa cctgacgggc gaggtcgacg cggacggctc
     1681 gtacacgctg ttcgcgacga cgtcgacggt cagcaacgag tcgacccacg accagggcgc
     1741 cgatccgaac gagatcgtct cgatccacct ggacgccgcc tcgaccgccg ccgacacctc
     1801 gttctccatc gtcgagaccg cggccgccgg cgagcgcttc ggcggcgtcg ccctgacgcc
     1861 ggtgccggag ccgggcaacg ccgcactgct gctggcgggc gttggcctgc tcgcgggcgc
     1921 ggcgcgccgt cgccgcgtcg gctgatcgac ccgatcggcc aacccccgcg ccgcattgag
     1981 gcggcgcaac gagtcgtccc gcgccgccgc cgatgatcga cgccgtcccg tcgatgcggc
     2041 cgggcgtgga cgcactccgc ctgcacgccg cgcccgggag cacgacatgt acgcacgcat
     2101 cctcgttccg atcgacggca gtccgacggc cgaatgcggg ctgggcgagg cgatccgcct
     2161 ggcccaggcc atgaggggca ggatcgtcgc gctgacggtc tcggccggct tcgtcggggc
     2221 gatggagatg gcctccgcgc gcgactacca ggagtcctcc gcgcggcagc tcaaggcctg
     2281 caccgatctc gtggatgccg ccgcctcgcg cgcccgcgat gccggcgtcg aatgcgacaa
     2341 ggtcgtcgcc gacaaccgcg ggcgctcggt ggcgcagacg atcctcgacg aatgcgcgac
     2401 gtggaactgc gacctggtcg tgatgggcac gcacggccgc cgcggcctga cgcgcatggt
     2461 gctgggcagc gacgccgagg cggtgctgcg cggggcgagg acgccggtga tgctggtgcg
     2521 cccgtccgag gcgcattgag cgcgtgcccg cgccgcgggc tcgaaggcgc ggcggggcgg
     2581 cggcctcagc gatcgggcgc g
//
LOCUS       DASVLN010000973         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_105893, whole genome shotgun sequence.
ACCESSION   DASVLN010000973 DASVLN010000000
VERSION     DASVLN010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105893"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VF453_22845"
     CDS             <1..734
                     /locus_tag="VF453_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7690560.1"
                     /translation="PAPAATPTPDAAPAPVAAPAAEKQTIDNPYGLTALWRDGGIIAK
                     INLVIMLIMSMGSWYIIFTKLWEQHKMLKAAENVGDTFWTAGSIKAGTNTLEEGSAFR
                     YIAESGLKSSEHHEGSLVDQIDRHTWISMSVSRAVENIQSRLSDGLAFLATVGSTAPF
                     VGLFGTVIGIYGALTQIGIAGQASIDKVAGPVGEALIMTAIGLFVAVPAVMGYNWLIR
                     RNKSVMERVRAFSGDLHNVLLAGKR"
     gene            785..1219
                     /locus_tag="VF453_22850"
     CDS             785..1219
                     /locus_tag="VF453_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7690561.1"
                     /translation="MAMNVGTGGDSDGEEQVNSTINTTPLVDVMLVLLIIFLITIPVV
                     TQSIQLELPKERNVVTVTKPENILLAVSKDGDLYWNTKKIDDIDQLVAMLTEKAKMKP
                     QPEVHIRGDQAARYESIGRVVFACQRAGIAKVGFITEPPPNS"
     gene            1233..1658
                     /locus_tag="VF453_22855"
     CDS             1233..1658
                     /locus_tag="VF453_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7690562.1"
                     /translation="MAMATSSAEDGDVMVDINTTPLIDVMLVLLIMLIITIPIQTHAV
                     KLNMPVDSHNAPPPKPPVVVEIAVDFDGTIGFGGEIVKDRADLETKLYRLTQMPDQPE
                     VHLLPNKLAPYKYVAEVMAEAQRLGVTKIGIVGNEQFTH"
     gene            1760..2971
                     /locus_tag="VF453_22860"
     CDS             1760..2971
                     /locus_tag="VF453_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690563.1"
                     /translation="MKPLRLLAAALVAAGLLHTPAAFAVSAAVAKPLKEASDLLRAGK
                     AKEALSKLNGVSGSGPEDSYMIDRMRGAAYQRMGENAAAVQALEGAFNTGKVPPGEAG
                     ALAESIAAAYAQLKNNAKAQQWAEKAKAAGDNSATLRELQAYLQGSSGDYATIARESA
                     AAVQSAEAAGRRPAEDDLLRLADAYRHTGNKAGDLQVKEKLVLNYPSNKQYVSIYLSD
                     LPGKPGFSPRFSLDLLRLRLASGNLTEEPDYMEMGQQLLQAKLPAEAKVVVDKGYAAG
                     VLGKGAQAAREQRLRDLTTKMLADQKSTLAARTTEAEAAKDGNDLVDIGTEYATLGEY
                     DKAVELIQQGMAKDKLKHPEDAKLRLGVAMLQSGKNRQKAVQTLRSVQGTDGAPEIAR
                     LYIALGAGTGQ"
BASE COUNT          587 a         1067 c          953 g          392 t
ORIGIN      
        1 cgccggcgcc cgccgcgacg cccacccccg acgccgcccc ggcgccggtc gccgcgcccg
       61 ccgccgagaa gcagaccatc gacaacccgt acggcctgac cgccctgtgg cgcgacggcg
      121 gcatcatcgc caagatcaac ctggtcatca tgctgatcat gtcgatgggc agctggtaca
      181 tcatcttcac gaagctgtgg gaacagcaca agatgctgaa ggccgccgag aacgtcggcg
      241 acaccttctg gaccgcgggc tcgatcaagg ccggcaccaa caccctggaa gaaggcagcg
      301 ccttccgcta catcgccgag agcggcctga agtccagcga gcaccatgaa ggttccctgg
      361 tcgaccagat cgaccgccac acctggatct cgatgagcgt ctcgcgcgcc gtggagaaca
      421 tccagtcgcg cctgtcggac ggcctggcct tcctcgcgac cgtcggctcg accgccccgt
      481 tcgtcggcct gttcggcacc gtcatcggca tctacggcgc gctgacgcag atcggcatcg
      541 ccggccaggc gtcgatcgac aaggtcgccg gccccgtcgg cgaagcgctg atcatgaccg
      601 ccatcggtct gttcgtcgcc gtgccggccg tgatgggcta caactggctg atccgccgca
      661 acaagagcgt catggagcgc gtgcgcgcct tctcgggcga cctgcacaac gtcctgctgg
      721 ccggcaagcg ctgagacgcc tggcgactgc acttgacgaa cgcccgtaat cgaaaggaac
      781 cctcatggcg atgaacgtcg gcacgggcgg tgacagcgac ggcgaagagc aggtcaactc
      841 caccatcaac accacgcccc tcgtggacgt gatgctggtg ctgctgatca tcttcctgat
      901 cacgatcccg gtcgtgacgc agtcgatcca actggaactg cccaaggaac gcaatgtcgt
      961 gaccgtgacg aagccggaga acatcctgct cgcggtctcg aaggacggcg acctgtactg
     1021 gaacacgaag aagatcgacg acatcgatca actggtggcg atgctgaccg agaaggccaa
     1081 gatgaagccc cagcccgagg tgcacatccg cggggaccag gcggctcgct acgagtcgat
     1141 cggtcgcgtc gtgttcgcat gccagcgcgc cggcatcgca aaggtgggct tcatcaccga
     1201 acccccgccg aacagctaag gagctcgaaa acatggcaat ggcgacaagc tccgcagaag
     1261 acggcgacgt gatggtcgac atcaacacca cgccgctgat cgacgtgatg ctggtgctgc
     1321 tgatcatgct gatcatcacg atcccgatcc agacgcacgc ggtgaagctg aacatgcccg
     1381 tggactcgca caacgcgccg ccgcccaagc cgccggtcgt cgtcgagatc gccgtcgact
     1441 tcgacggcac catcggcttc ggcggagaga tcgtgaagga ccgggcggac ctcgagacga
     1501 agctgtaccg tctcacgcag atgcccgatc agcccgaagt ccacctgctg ccgaacaagc
     1561 tggccccgta caagtacgtg gccgaggtga tggccgaggc ccagcgcctc ggggtcacca
     1621 agatcggcat cgtcgggaac gaacagttca ctcactgact ctgagtcgaa cccgcctcac
     1681 gaaggcgcac tgctcgcagt gcgccttcgc cctttcagac ggcctcccag caggcacgaa
     1741 cacaggcaga caaacactca tgaagcccct ccgactcctc gccgccgcgc tggtcgcggc
     1801 cggcctcctg cacaccccgg ccgcgttcgc cgtcagcgcg gcggtggcca agccgctcaa
     1861 ggaagcgagc gacctgctgc gcgccggcaa ggccaaggaa gcgctgtcca agctcaacgg
     1921 cgtctcgggc tccggtccag aagacagcta catgatcgac cgcatgcgcg gcgcggccta
     1981 ccagcgcatg ggcgagaacg ccgccgccgt gcaggcgctc gaaggcgcgt tcaacaccgg
     2041 caaggtgccg ccgggcgagg ccggcgcgct ggccgagtcg attgccgccg cctacgcgca
     2101 gctgaagaac aacgccaagg cccagcagtg ggccgagaag gccaaggccg ctggcgacaa
     2161 cagcgccacg ttgcgcgagt tgcaggccta cctccagggc tccagcggcg actacgcgac
     2221 gatcgcgcgc gaaagcgccg ccgccgtcca gtccgccgag gccgcgggcc gccgcccggc
     2281 cgaggacgac ctgctgcgcc tggccgacgc ctaccgccac acgggcaaca aggcgggcga
     2341 cctgcaggtc aaggaaaagc tcgtcctgaa ctatccctcg aacaagcagt acgtcagcat
     2401 ctacctgtcc gacctcccgg gcaagccggg cttctcgccg cgcttctcgc tggacctgct
     2461 gcgcctgcgc ctcgcttcgg gcaacctgac cgaggagccc gactacatgg agatgggcca
     2521 gcagctgctg caggccaagc tgcccgccga agccaaggtc gtcgtcgaca agggctacgc
     2581 tgcaggcgtc ctgggcaagg gcgcccaggc cgcgcgcgag cagcgcctgc gcgacctgac
     2641 caccaagatg ctcgccgacc agaagtccac gctcgcggcg cgcacgaccg aggccgaggc
     2701 agccaaggac ggcaacgacc tggtcgacat cggcaccgag tacgccaccc tcggcgagta
     2761 cgacaaggcc gtcgagctga tccagcaggg catggccaag gacaagctca agcaccccga
     2821 ggacgccaag ctgcgcctgg gcgtggccat gctgcagtcg ggcaagaacc gccagaaggc
     2881 ggtgcagacg ctgcgcagcg tccagggcac cgacggcgcg cccgagatcg cgcgcctgta
     2941 catcgcgctg ggcgcgggca cgggccagtg accgggtgcc ggcccggcgc gcccgtccg
//
LOCUS       DASVLN010000974         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_105919, whole genome shotgun sequence.
ACCESSION   DASVLN010000974 DASVLN010000000
VERSION     DASVLN010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105919"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1112)
                     /locus_tag="VF453_22865"
     CDS             complement(222..1112)
                     /locus_tag="VF453_22865"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7690564.1"
                     /translation="MPAAAASSSVARPALALHGHHVRRLEAVVDFVDAHLDGELSLER
                     LAGVAAISPFHFHRLFHLWSGETLSEFVRRRRLDVAAGRLRHCPGEKITAISLNCGFA
                     SPEGFARAFRERFGATPSTWRDRDREAEPDLGGMPPVVVAREPEMEILFMRARGDFAV
                     MAPVLWDRFMSALRALGLVGQPLLFMGLDDPAIAGPGRCRMDACVQLPAGFGLVHACP
                     QPLLHKRIPGRWVARLRYDGPAAHIGEGWRALLHQWLPQAAYQLAIGPFHERYDPAFG
                     VPGTRHVRCELAMPVEPRVA"
     gene            complement(1117..2139)
                     /locus_tag="VF453_22870"
     CDS             complement(1117..2139)
                     /locus_tag="VF453_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7690565.1"
                     /translation="MPSKEKSLTEPATLRDVARLAGVSAATVSRILNGTARVTDDKRS
                     AVERAIERLQFRPNFAAQSLRSGHTRTIGVLTQELESPYFTRGARGIEDGLQGSEYAP
                     IVVPGHWNSVEELDRARLLIARRVDAMVILGGNLQDEQVSDMARTLPIAITGRDLHAA
                     NVFSLHCDQVEGACRAVRHLIELGHTRIAHITGPVSYPDAVDRREGYMRAHSEAGLPV
                     DPRLLRVGTYLETGGAQAMTQLLDAGHEFTAVFCANDQTLWGARQVLYERGIRVPDDI
                     SLVGFDDLPQSAYMTPAVTTVHQPIYEMGRAAAHKLLEALGATAPRGEPAPELRLVVR
                     GTTRAV"
     gene            2491..>2831
                     /locus_tag="VF453_22875"
     CDS             2491..>2831
                     /locus_tag="VF453_22875"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7690566.1"
                     /translation="MLAGAARVTLAVAAFPDLDRSVKIAIESWAASHPDVEVRLTTRA
                     IADHHTAMTTAIATGANLPDVMAIDMDYLGKFVQAAGLEDLDAAPYDAAAVAPQLARF
                     TLGPATGLGGRL"
BASE COUNT          418 a          990 c         1024 g          399 t
ORIGIN      
        1 gggacgggcg ccggtgcgct ggcgtcgccg ccgcagccgg tcaggcccag cgaggcgacg
       61 agccccatga gcacacccag tcgcgcgagc ggggtcgtgg tatccatcga gatgtctcca
      121 atgtggttgg ctggcatcgt catcgtgccg acgccggcgc ccgcgcaccg gtgcgcgctt
      181 gcggaggacc tgatggatct tgcgaagttg cgggcggctg cttaggcgac gcgcggctcg
      241 accggcatgg ccagctcgca gcgcacgtgg cgggtgccgg gcacaccgaa cgcagggtcg
      301 tagcgctcgt ggaacgggcc gatcgccagc tggtacgcgg cctgcggcag ccactggtgc
      361 agcagggcac gccagccctc gccgatgtgc gccgccgggc cgtcgtagcg cagcctggcg
      421 acccagcgcc ccgggatgcg cttgtgcagc agcggctgcg ggcacgcgtg gacgaggccg
      481 aagcccgcgg gcagttgcac gcaggcgtcc atgcggcatc tcccggggcc ggcgatcgcg
      541 gggtcgtcca ggcccatgaa gagcagcggc tgccccacca gtcccaaggc gcgcagcgcg
      601 ctcatgaagc gatcccacag caccggcgcc atgacggcga agtcgccgcg ggcgcgcatg
      661 aacaggatct ccatctccgg ctcgcgcgcc acgaccaccg gcggcatgcc gccgaggtcg
      721 ggctcggcct cgcggtcgcg atcgcgccag gtcgaaggcg tcgcgccgaa gcgctcgcgg
      781 aacgcgcgcg cgaagccctc cggcgacgcg aagccgcagt tcagcgagat cgccgtgatc
      841 ttctcgcccg ggcagtggcg aagccgcccc gccgccacgt cgagccgccg ccgacgcacg
      901 aactcgctga gcgtctcgcc gctccacagg tggaacaggc gatggaagtg gaacggagag
      961 atcgcggcga cgccggccag gcgctcgagc gagagttcgc cgtcgagatg agcgtcgacg
     1021 aagtcgacca cggcctccag ccgccgcacg tgatggccgt gcagcgccag cgctgggcgc
     1081 gcgacgctgc tcgacgcggc ggccgccggc atggcttcag acggcgcgcg tggtgccgcg
     1141 caccacgagc cgaagttcgg gtgccggctc gccgcgcggt gcggtcgcgc cgagggcctc
     1201 gagcagcttg tgcgccgccg cgcgccccat ctcgtagatc ggctggtgca ccgtcgtcac
     1261 cgccggcgtc atgtaggccg actggggcag gtcgtcgaaa cccacgagcg agatgtcgtc
     1321 cggcacgcgg atgccgcgct cgtacagcac ctggcgcgcg ccccacagcg tctggtcgtt
     1381 cgcgcagaag acggcggtga actcgtggcc tgcgtcgagc agttgcgtca tcgcctgggc
     1441 gccgccggtc tccaggtagg tgcccacccg cagcaaccgc ggatcgaccg gcaggccggc
     1501 ctcgctgtgc gcgcgcatgt agccttcgcg gcggtcgacc gcgtcggggt aggagacggg
     1561 cccggtgatg tgcgcgatcc gcgtgtgccc cagctcgatc aggtgccgca cggcgcggca
     1621 tgcgccttcg acctggtcgc agtgcagcga gaagacgttg gccgcgtgca ggtcgcgtcc
     1681 ggtgatggcg atgggcagcg tgcgcgccat gtcgctcacc tgctcgtcct gcaggttgcc
     1741 gccgaggatc accatcgcgt cgacccggcg cgcgatcagc aggcgggcgc gatcgagctc
     1801 ctcgacggaa ttccagtggc ccggcacgac gatgggagcg tattcgctgc cttgcaggcc
     1861 gtcctcgatg ccgcgcgcgc cgcgggtgaa gtacggcgac tcaagttcct gcgtcagcac
     1921 gccgatggtc cgggtatggc cgctgcgcag gctctgcgcg gcgaagttcg ggcggaactg
     1981 caggcgctcg atggcgcgct cgacggcgga tcgcttgtcg tcggtgacgc gcgccgtgcc
     2041 gttgaggatg cgcgacacgg tggcggcgga cacgccggcc aggcgcgcga cgtcgcgcag
     2101 cgtggcgggc tcggtgaggg acttttcctt ggaaggcatg gcgggctcgg cggaattgag
     2161 cagcgcggcg agaaggcgcg gcgcacgcat gttagcgagg ccgcgccgcg cccgccgggc
     2221 tcgggccgat gcggcggaag tctatccatg aaatcgattt ctgacgagat ggcgaaacct
     2281 cgggaggtat cactgatggt cgaacgtgtg ccaaccgtca cacgaacgct caccactcgt
     2341 cagggtattc accaactcgg ccatgaaccc attgacgacc atgacagcgt tgtctcacac
     2401 tgagcactga aatcgatatc aagcctgctc aacccgccca tgcgatcgcg cttcctgctt
     2461 gtcctcgccg cttgcctgac cgcacttccg atgctcgcgg gcgccgcccg cgtcacgctg
     2521 gccgtggcgg ccttcccgga tctcgaccgc agcgtgaaga tcgcgatcga gtcgtgggcg
     2581 gcttcgcatc cggacgtcga ggttcgcctg acgacacgag cgatcgcgga ccaccacacc
     2641 gcgatgacga ccgcgatcgc cacgggcgcc aacctgcccg acgtgatggc catcgacatg
     2701 gactatctcg gcaagttcgt gcaggccgcc ggccttgagg acctggacgc cgcgccgtac
     2761 gacgcggccg ccgtcgcgcc gcagctcgcg cggttcaccc tcggccccgc gacgggcctg
     2821 ggcggccgcc t
//
LOCUS       DASVLN010000975         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_105940, whole genome shotgun sequence.
ACCESSION   DASVLN010000975 DASVLN010000000
VERSION     DASVLN010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105940"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1034
                     /locus_tag="VF453_22880"
     CDS             72..1034
                     /locus_tag="VF453_22880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7690567.1"
                     /translation="MSLVPQRPLVLDHLRSFEAVARRAGFGAAAQELHVTQSAVSRQI
                     KSLEEELGAPLFTRGTRRVELTQAGQLLLRAITPALSRIDSAVRQIRQQRGRAQVSLS
                     TFPSFASLWLLPRLPTFERQQKAIDIRITAIDRLIEIDDPDYDLLLRQCRPEDAPPGA
                     RRMFGEVLTPVIGASLVDAIARGAAPPLDEPADLAAHTLIAMEDDRPGIELLTWNYWL
                     EQRGLGQLEPRRWISVNFTHQEVQTALAGQGVALARLPLIHDLLQRGDLVEPFGSAGR
                     LASPHAYWHVPLPGARPRPELDSFMLWLEEATHATRVAIGESAG"
     gene            complement(1054..>1954)
                     /locus_tag="VF453_22885"
     CDS             complement(1054..>1954)
                     /locus_tag="VF453_22885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690568.1"
                     /translation="DTQPASLLGAAETAPQMLDTAPMTMDSAPASLLGSTTDGSGHMI
                     LDVPLPIDEPEADAQAAGAMLPYEQASGPESEFAAAPGAVPADAAMRGLISSLRRSFG
                     AGRLLQAGRWVRLAHVSPPVKIQTNAADPMRVLYAKQDVLIRKGRIVWGAAVPLDKAL
                     QAPGKEDLPGLLVYSQDAWFDARPAELAAVAMRLAVLRTAPAATPELRRLGLQVRNDS
                     GRPLDVKLPPELSTHGVVLTSFMGVRSHLPDTVLAADWFPVLVHEASVVPLIVPSMFW
                     SAAMRAAWDARKLAVAAAVGQTA"
BASE COUNT          258 a          759 c          703 g          234 t
ORIGIN      
        1 cccgcccgcg ccgcggcgaa gaaggccgcg accgcgaaga ccgccgcggc ccggccgagg
       61 acgccgcgcg catgagcctg gtgccgcaac gcccgctggt gctcgaccac ctgcgcagct
      121 tcgaggccgt cgcccgccgc gccggcttcg gcgccgcggc gcaggagctg cacgtcacgc
      181 agtccgccgt cagccgccag atcaagagcc tcgaggagga gctcggcgcg cccctgttca
      241 cgcgcggcac gcggcgggtc gagctcacgc aggccggcca gctgctgctg cgcgcgatca
      301 cgccggcgct cagccgcatc gacagtgccg tgcgccagat ccgccagcag cgcgggcgcg
      361 cgcaggtgag cctgtcgacc ttcccgtcct tcgcctcgct gtggctgctg ccgcgcctgc
      421 ccaccttcga gcgccagcag aaggcgatcg acatccgcat caccgcgatc gaccgcctga
      481 tcgagatcga cgacccggac tacgacctgc tgctgcgcca gtgccgcccg gaggatgccc
      541 cgcccggcgc gcgccgcatg ttcggcgagg tgctgacgcc ggtgatcggc gcgagcctgg
      601 tcgacgcgat cgcccgcggc gccgcgccgc ccctggacga gccggccgac ctcgccgccc
      661 acacgctgat cgcgatggag gacgaccgcc ccggcatcga gctcctgacc tggaactact
      721 ggctggagca gcgcggcctc ggccagttgg agccgcggcg ctggatcagc gtcaacttca
      781 cgcaccagga ggtgcagacg gcgctcgccg gccagggcgt cgcactggcg cggctgccgc
      841 tgatccacga cctgctgcag cgcggcgatc tcgtcgagcc tttcggcagc gccggccggc
      901 tcgcctcgcc gcacgcctac tggcacgtgc cgctgcccgg cgcgcgcccg cggccggagc
      961 tggacagctt catgctctgg ctggaggagg ccacgcacgc gacgcgcgtg gcgatcggcg
     1021 agtcggcggg ctgaacgccc gcccgggacc gggtcaggcg gtctggccga ccgcagccgc
     1081 caccgccagc ttgcgcgcgt cccacgcggc gcgcatcgcc gcgctccaga acatgctggg
     1141 cacgatcagc ggcacgacgc tggcctcgtg cacgagcacc gggaaccagt ccgcggcgag
     1201 caccgtgtcg ggcaggtgcg agcgcacgcc catgaagctg gtgagcacga cgccgtgcgt
     1261 gctgagctcc ggcggcagct tgacgtccag cggacggccc gagtcgttgc gcacctgcag
     1321 gcccagccgc cgcagctcgg gcgtcgccgc cggcgccgtc ctcagcacgg ccagccgcat
     1381 cgcgacggcg gcgagctccg ccgggcgcgc gtcgaaccag gcgtcctggc tgtagacgag
     1441 cagtcccggc aggtcctcct tcccgggggc ctgcagcgcc ttgtccaacg gtaccgccgc
     1501 gccccagacg atgcggccct tgcggatcag cacgtcctgc ttggcgtaga gcacgcgcat
     1561 tggatcggca gcattggtct ggatcttcac cggcggcgac acgtgcgcga gccgcaccca
     1621 gcgcccggcc tgcaggaggc ggccggcgcc gaacgagcgc cgcagcgacg agatcagccc
     1681 gcgcatcgcc gcatcggccg gcaccgcgcc cggggccgcc gcgaactcgg actccgggcc
     1741 ggacgcctgc tcgtacggca gcatcgcgcc ggccgcctga gcgtcggcct ccggctcgtc
     1801 gatcggcagc ggcacgtcga ggatcatgtg gccggagccg tccgtcgtcg agcccagcag
     1861 cgaggcgggc gcgctgtcca tggtcatcgg cgccgtgtcg agcatctgcg gcgcggtctc
     1921 ggcggcgccg agcagcgagg cgggctgggt gtcc
//
LOCUS       DASVLN010000976         8313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_482250, whole genome shotgun sequence.
ACCESSION   DASVLN010000976 DASVLN010000000
VERSION     DASVLN010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8313
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482250"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="VF453_22890"
     CDS             <1..168
                     /locus_tag="VF453_22890"
                     /inference="COORDINATES: protein motif:HMM:NF019389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEX7690569.1"
                     /translation="AVVASAGAASRPATPQRTRMKACNADARTRDLHGDPRRAFMKQC
                     LSGKARPADLF"
     gene            complement(184..1089)
                     /locus_tag="VF453_22895"
     CDS             complement(184..1089)
                     /locus_tag="VF453_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7690570.1"
                     /translation="MDKLKQIESFVAVAQRGSLTAAAQAEGVAPAVVSRRLDGLEARL
                     GVKLLARTTRRVSLTHEGSAFLEDCQRLLADFASAEASVSAGGVKASGHLRVTAPAGF
                     GRRHVAPLVPAFLSEHPDVSLSLNLSDRLVDIVNEGVDCAVRVGDLPDSSLVSVRLAD
                     NRRLCVAAPSYLRRAGTPQAPSDLARHACLILSSEASQTRGWAFTVDGETTHLRLAGR
                     LDCTDGQVLHEWCLAGLGIAWRSTWEVRDDLQAGRLVSVLDDFAAPPNGIYAVFPHAR
                     HLALRVRLWIDFLKHSYGDARYWSR"
     gene            1178..1900
                     /locus_tag="VF453_22900"
     CDS             1178..1900
                     /locus_tag="VF453_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX7690571.1"
                     /translation="MESAAAPNPRAAASAERALPRPKAVLFDVYGTLLDVYSVALRAE
                     QMFPGEGDRLARLWRERQIEYSRLTSMSGRYRPFWELTRDALRFCAAALELPLDAAGE
                     DALMNEYRHLSAFPENRGVLQALAARGIRAGVLSNGDPEMLAVSVRSAGLTELIDPIL
                     SVHATRRYKTDPAAYALGPVALGLAAPEILFVSSNCWDAIGATWYGFTTLWINRAGAP
                     LEQLGTAPTRVGQSLRDVLEFF"
     gene            1958..3544
                     /gene="aceB"
                     /locus_tag="VF453_22905"
     CDS             1958..3544
                     /gene="aceB"
                     /locus_tag="VF453_22905"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX7690572.1"
                     /translation="MSPTLPAGMTISAPILPGFESVLTPEALAFVADLHRTFEPRRQE
                     LLAARVERAARLDAGERPDFLEATRHIREGDWKIAPLPRALECRRVEITGPVEAKMVI
                     NAFNSGADSYMTDFEDSNAPKWANQIQGQINLSQAIRRTLRLEQNGKTYQLNDKIATL
                     QVRPRGWHLDEKHVKVDGQRVSGGIFDFGLFLFHNAKEQIARGAGPFFYLPKMESHLE
                     ARLWNDIFVRAQDKLGLPQGTIKATVLIETILAAFEMDEILYELREHSAGLNAGRWDY
                     IFSCIKKFKVDTDFCLAERAKVTMTSPFMRAYALLLLKTCHKRGAPAIGGMSALIPIK
                     NDPEKNAVAMAGIVADKKRDATDGYDGGWVAHPGLVEPAMKEFLAVLGDRPNQWDKQR
                     DDVAVSAAELLDFQPETPITEAGLRNNINVGIHYLGAWLGGNGCVPIHNLMEDAATAE
                     ISRSQVWQWIRSPKGVLDDGRKVTAELVRGLIPEELARIQGTGAVGEFERAAQIFEKM
                     STDTAFAEFLTLPLYEETSF"
     gene            complement(3587..5278)
                     /locus_tag="VF453_22910"
     CDS             complement(3587..5278)
                     /locus_tag="VF453_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004547236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7690573.1"
                     /translation="MSQPSPTSLALQRLFHWERNAPDRTAFTQPVGEHLLKEFSWKDV
                     VDEARRMAAFLQQQGFEPGARIAILSKNCAWWLMSDWAIWMAGYVSVPLYPTLAAGTI
                     AHILEHSDARLLFVGKLDDWDTMRPGVPSGLPCISYPLSPEDVRARVDMQWDAIVART
                     LPLAGHPVRAGGELATLMYTSGTTGTPKGVMHSFDSFAWAIDAGLKRVSMDADSRMLS
                     YLPLAHVAERTLVEHGQLATGMHLYFAESLETFARDLQRARPTTFFSVPRLWIKFQQG
                     VHAKMPPQRLERLLKVPIAGRLLRRKILKAMGLDHCQYAAGGAAPMPPDLLRWYSQLG
                     LDIIEVYGMTENCGVSHSTLPEVRRPGTVGVPYDGVESRLDPATGEIQMRCGSLMLGY
                     YKEPALTAGAFTADGWLRTGDKGLIDADGCLRITGRVKDLFKTSKGKYVAPAPIEDRL
                     VGHPAIEACCVTGANYPQPFALVMLNADAAAKAADPASRAALEDSLANHLLTVNHALD
                     PHEQLEVLVLMTEPWTIDNGLITPTFKVKRNIVEERWSARYDGWTSRGSKIVWAE"
     gene            complement(5441..6736)
                     /gene="gshA"
                     /locus_tag="VF453_22915"
     CDS             complement(5441..6736)
                     /gene="gshA"
                     /locus_tag="VF453_22915"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX7690574.1"
                     /translation="MVPHLVTALTGPINELEQRVLESAPAIERWFRLEWMEHTPPFYT
                     SVDVRNAGYKLAPVDTNLFPGGFNNLTEQMMPLAIQAAMAAIEKICPEAKNLLLIPEN
                     HTRNTFYLMNVKQLVRIFSAAGLNVRLGSLDPAVKEPTTLALPDGSSLVVEPLERSAR
                     RLGLKDFDPCTVLLNNDLSAGLPKVLENLHEQYLLPPLHAGWAVRRKTNHFHSYEEVA
                     KKFAKLLGMDPWLINPMYTHCGQVDWAAGTGLECVQTNVDALLTKIRRKHKEYGINEK
                     PFVVVKADNGTYGMGVMTVRDAKELDEVGRRTRNKMDVVKDGQHVTEVIIQEGVPTYE
                     RVNDAVAEPVVYMIDRYVVGGFYRMHAERGIDENLNAPGSSFVPLAFAESSHLPRPGE
                     KPGVSAPNRFYMYGVIGRLAMLAASYELEATDPEAEVYE"
     gene            complement(6927..>8313)
                     /gene="amt"
                     /locus_tag="VF453_22920"
     CDS             complement(6927..>8313)
                     /gene="amt"
                     /locus_tag="VF453_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX7690575.1"
                     /translation="AAAASAPDAAASAPTETVNKGDVSWMLVSTALVIMMSIPALGLF
                     YGGLVRSKNMLSVLMQVFVTFSLIVVLWALYGYSLAFTQGSGWSSPFFGGFSRLGLSG
                     TFDPMKGVFSNAATFSKGVVLPELVFVVFQATFAAITCCLIVGSFAERMKFSAVLAFM
                     VLWFTFSYLPIAHMVWFWQGPDAYTSKSVVDAQNATAGLIWQWGALDFAGGTVVHINA
                     AVAGLVGAYMVGKRIGYGKEAFTPHSTTLTMVGASLLWVGWFGFNAGSALEAGTSATL
                     AFVNTFTATAAAVLAWSLGEALMKGKASMLGAASGAVAGLVAITPAAGNVGLMGAIVL
                     GFIAGFAGLWGVHGLKKLLGADDTLDVFGVHGVCGIVGALLTGILNNQSLGGPGMVTD
                     WVSMTTGSNGIWDQFVIQAKAVGVTVAWSGVVSFVAYKLVDLTIGLRVTEDEEREGLD
                     ITSHGETAYAK"
BASE COUNT         1267 a         2907 c         2914 g         1225 t
ORIGIN      
        1 gcggtcgtcg cgtcggccgg cgccgcctcg cggcccgcca cgccgcagcg cacccgcatg
       61 aaggcctgca acgcggacgc gcgcacgcgc gacctccacg gcgacccgcg gcgcgcgttc
      121 atgaagcagt gcctgtccgg caaggcgcgg cccgcggacc tgttctgacc ggcgccggcc
      181 gcctcagcgc gaccagtagc gcgcgtcgcc gtagctgtgc ttgaggaagt cgatccacaa
      241 ccgcacgcgc agcgcgaggt ggcgcgcgtg cgggaacacc gcgtagatcc cgttgggcgg
      301 cgcggcgaag tcgtcgagga cgctcacgag gcgtccggcc tgcaggtcgt cgcggacttc
      361 ccaggtgctg cgccaggcga tcccgagccc ggccaggcac cactcgtgca gcacctggcc
      421 gtcggtgcag tccagccggc cggccaggcg caggtgcgtg gtctcgccgt cgaccgtgaa
      481 ggcccagcct cgggtctggc tcgcctccga gctgaggatc aggcacgcgt ggcgcgcgag
      541 atccgacggt gcctgcggcg tgccggcgcg cctcaggtag gacggcgcgg ccacgcacag
      601 gcggcggttg tcggccagcc gcacgctcac gaggctggag tcgggcaggt cgccgacccg
      661 cacggcgcag tcgacgccct cgttgacgat gtcgacgagc cggtccgaca ggttcagcga
      721 caggctgacg tccggatgct ccgacaggaa ggcgggcacc agcggcgcga cgtggcgccg
      781 cccgaagccg gccggcgcgg tgacgcgcag gtggccgctc gccttcacgc cgccggccga
      841 gacgctggcc tcggcgctcg cgaagtcggc gagcaggcgc tggcagtcct cgaggaaggc
      901 gctgccctcg tgcgtcagcg agacgcgacg cgtggtgcgc gcgagcagct tgacgcccag
      961 ccgcgcctcc agcccgtcga gccgccggct gaccaccgcc ggcgccacgc cctcggcctg
     1021 cgccgccgcc gtcaggctgc cgcgctgggc gacggccacg aaggactcga tctgcttcag
     1081 cttgtccacg cgcgcatccg cttttgcaaa aaagtcacca atcaatctcg aattatggcg
     1141 ggaatcgtca tccaggcttg gaatagattc gcattccatg gaatccgccg ccgctccgaa
     1201 tccccgcgcc gccgcgtccg ccgagcgcgc gctcccgcgt cccaaggccg tgctgttcga
     1261 tgtgtacggc acgctgctgg acgtctacag cgtcgccctg cgcgcggaac agatgttccc
     1321 gggcgagggc gaccgcctcg cgcggctgtg gcgcgagcgg cagatcgagt attcgcgcct
     1381 gacctcgatg agcggccgct accgcccgtt ctgggagctg acgcgcgacg cgctgcgctt
     1441 ctgcgctgcg gcgctggagc tgccgctgga cgccgccggc gaggacgcgc tgatgaacga
     1501 gtaccgccac ctctcggcct ttcccgagaa ccgcggcgtg ctgcaggcgc tggccgcgcg
     1561 cgggatccgc gccggcgtgc tgtccaacgg cgacccggag atgctcgcgg tctccgtgcg
     1621 cagcgccggg ctgaccgagc tgatcgaccc gatcctgagc gtgcatgcga cgcgccgcta
     1681 caagaccgat cccgccgcct acgcgctcgg cccggtcgcg ctgggactgg cggcgccgga
     1741 gatcctgttc gtctcgtcca actgctggga cgcgatcggc gcgacctggt acggcttcac
     1801 gacgctgtgg atcaaccgcg ccggtgcgcc cctcgagcag ctgggcaccg cgcccacgcg
     1861 cgtcgggcag tcgctgcgcg acgtcctcga attcttctga cgcggccgcc cgcgtcgcct
     1921 tgcatcttcc gcacctcttc acttccagga gttccccatg agcccgaccc tgcccgccgg
     1981 catgaccatc tccgccccga tcctgcccgg attcgagtcc gtgctgacgc ccgaggcgct
     2041 cgccttcgtc gccgacctcc accgcacgtt cgagccgcgc cgccaggagc tgcttgccgc
     2101 ccgcgtcgag cgtgcggcgc gcctggacgc gggcgagcgc ccggacttcc tcgaggccac
     2161 gcgccacatc cgcgagggcg actggaagat cgcgccgctg cccagggcgc tcgagtgccg
     2221 ccgcgtggag atcaccgggc cggtcgaggc caagatggtc atcaacgcgt tcaactcggg
     2281 cgcggacagc tacatgaccg acttcgagga cagcaacgcg ccgaagtggg ccaaccagat
     2341 ccagggccag atcaacctgt cgcaggcgat ccgccgcacg ctgcggctcg agcagaacgg
     2401 caagacctac cagctcaacg acaagatcgc gacgctgcaa gtgcgcccgc gcggctggca
     2461 cctcgacgag aagcacgtga aggtcgacgg gcagcgcgtc tcgggcggca tcttcgactt
     2521 cgggctgttc ctgttccaca acgcgaagga gcagatcgcg cgcggcgccg gcccgttctt
     2581 ctacctgccc aagatggaga gccatctcga ggcgcgcctg tggaacgaca tcttcgtgcg
     2641 ggcgcaggac aagctgggcc tgccgcaggg cacgatcaag gcgacggtgc tgatcgagac
     2701 catcctcgcc gcgttcgaga tggacgagat cctgtacgag ctgcgcgagc acagcgcggg
     2761 cctgaacgcc ggccgctggg actacatctt cagctgcatc aagaagttca aggtcgacac
     2821 cgacttctgc ctggccgaac gcgcgaaggt cacgatgacc tcgccgttca tgcgcgccta
     2881 cgcgctgctg ctgctcaaga cctgccacaa gcgcggcgcg ccggccatcg gcggcatgag
     2941 cgcgctgatc ccgatcaaga acgaccccga gaagaacgcc gtggccatgg ccggcatcgt
     3001 cgccgacaag aagcgcgacg cgaccgacgg ctacgacggc ggctgggtcg cgcacccggg
     3061 cctggtcgag ccggcgatga aggagttcct ggccgtgctc ggcgaccgcc cgaaccaatg
     3121 ggacaagcag cgcgacgacg tggccgtctc cgccgccgag ctgctcgact tccagcccga
     3181 gaccccgatc accgaggccg gcctgcgcaa caacatcaac gtcggcatcc attacctggg
     3241 cgcctggctg ggcggcaacg gctgcgtgcc gatccacaac ctgatggagg acgccgcgac
     3301 cgcggagatc agccgttcgc aggtctggca gtggatccgc agcccgaagg gcgtgctgga
     3361 cgacggccgc aaggtgaccg ccgagctggt gcgcggcctg atccccgagg agctggcgcg
     3421 catccagggc acgggcgccg tcggcgagtt cgagcgcgcg gcgcagatct tcgagaagat
     3481 gagcaccgac accgcgttcg cggagttcct gaccctgccg ctgtacgaag agacctcgtt
     3541 ctgatcggat cgccggggcc gggtgcgcgc gtgacgcgtg ccccggtcac tccgcccaga
     3601 cgatcttgct gccgcgcgag gtccagccgt cgtagcgcgc cgaccagcgc tcctcgacga
     3661 tgttgcgctt gaccttgaag gtgggcgtga tgaggccgtt gtcgatcgtc cagggctcgg
     3721 tcatcagcac cagcacctcg agctgctcgt gcgggtcgag cgcatggttg accgtcagca
     3781 ggtggttggc gagggagtcc tccagcgccg cgcggctggc cgggtcggcg gccttggcgg
     3841 cggcatcggc gttgagcatg accagcgcga acggctgcgg gtagttcgcg cccgtgacgc
     3901 agcaggcctc gatcgccggg tggccgacca gcctgtcctc gatcggcgcc ggggcgacgt
     3961 acttgccctt gctcgtcttg aacaggtcct tcacccggcc ggtgatgcgc aggcagccgt
     4021 cggcgtcgat caggcccttg tcgccggtgc gcagccagcc gtcggccgtg aaggccccgg
     4081 cggtcagcgc cggctccttg tagtagccca gcatcagcga gccgcagcgc atctggatct
     4141 cgccggtcgc cgggtcgagg cgggactcga cgccgtcgta gggcacgccc accgtcccgg
     4201 ggcgccgcac ctcgggcagc gtggagtggg agacgccgca gttctcggtc atgccgtaga
     4261 cctcgatgat gtccagcccc agctgcgagt accagcgcag caggtccggc ggcatcggtg
     4321 cggcgccgcc cgcggcgtac tggcagtggt ccaggcccat cgccttgagg atcttgcgcc
     4381 gcagcagccg tccggcgatc ggcaccttca gcaggcgctc caggcgctgc ggcggcatct
     4441 tcgcgtgcac gccctgctgg aacttgatcc acagccgcgg caccgagaag aaggtcgtcg
     4501 gccgcgcccg ctgcaggtcg cgcgcgaagg tctccaggct ctccgcgaag tacaggtgca
     4561 tgccggtcgc gagctggccg tgctcgacga gcgtgcgctc ggcgacgtgg gccagcggca
     4621 ggtagctgag catgcgggag tcggcatcca tggagacgcg cttcaggccg gcgtcgatcg
     4681 cccaggcgaa gctgtcgaag ctgtgcatca cgccctttgg cgtcccggtg gtgcccgagg
     4741 tgtacatcag cgtcgcgagc tcgccgcccg cgcgcaccgg gtgcccggcc agcggcagcg
     4801 tgcgcgccac gatggcgtcc cactgcatgt cgacgcgggc gcgcacgtcc tcgggcgaga
     4861 gcgggtagct gatgcagggc agcccggagg gaacgccggg gcgcatcgtg tcccagtcgt
     4921 cgagcttgcc gacgaacagg agccgcgcgt cgctgtgttc gaggatgtgc gcgatcgtgc
     4981 cggccgccag cgtcgggtac agcggaaccg agacatagcc ggccatccag atcgcccagt
     5041 cgctcatcag ccaccaggcg cagttcttgg acaggatcgc gatgcgcgcg cccggctcga
     5101 agccctgctg ctggaggaag gccgccatgc ggcgcgcctc gtctacgacg tccttccagc
     5161 tgaattcctt gagcaggtgc tcgcccaccg gctgggtgaa cgccgtgcgg tcgggggcgt
     5221 tgcgctccca gtggaagagg cgctgcaggg cgagggacgt cggcgaaggc tggctcatgg
     5281 cgcgggcacc ggtggggcgt tcaggccaga ctagtgccgg tgcgccagcc gcgccatggt
     5341 gaaagcccgt gaagcgcagc gcgcggcgcg ccggtgcgcc gcgcggccag ggcaatggcc
     5401 gcggcggcca gtcggcgggg gcgcgcgtgg cggccggcgc ctactcgtag acctcggcct
     5461 cggggtcggt cgcctcgagt tcgtaggacg ccgcgagcat cgcgagccgt ccgatcacgc
     5521 cgtacatgta gaagcggttg ggcgcgctga cgccgggctt ctcgccgggg cgcggcaggt
     5581 gcgagctctc ggcgaacgcg agcggcacga agctggagcc gggcgcgttc aggttctcgt
     5641 cgatgccgcg ctccgcgtgc atgcggtaga agccgccgac gacgtagcgg tcgatcatgt
     5701 agaccaccgg ctcggcgacg gcgtcgttga cgcgctcgta ggtcggcacg ccttcctgga
     5761 tgatgacctc ggtgacgtgc tggccgtcct tgaccacgtc catcttgttg cgcgtgcggc
     5821 ggccgacctc gtcgagctcc ttggcgtcgc gcacggtcat cacgcccatg ccgtaggtgc
     5881 cgttgtcggc cttgacgacg acgaagggct tctcgttgat gccgtattcc ttgtgcttgc
     5941 ggcggatctt ggtcagcagg gcgtcgacgt tggtctgcac gcactccagc cccgtgcccg
     6001 cggcccagtc gacctggccg cagtgcgtgt acatcgggtt gatcagccac ggatccatcc
     6061 cgagcagctt ggcgaacttc ttggcgactt cctcgtagct gtggaagtgg ttcgtcttgc
     6121 ggcgcaccgc ccagccggcg tgcagcggcg gcagcaggta ctgctcgtgc aggttctcga
     6181 gcaccttggg cagcccggcc gagaggtcgt tgttcagcag cacggtgcag gggtcgaagt
     6241 ccttcagccc cagccgccgc gccgagcgct ccagcggctc gacgaccagc gagctgccgt
     6301 ccggcagcgc gagcgtcgtc ggctccttga cggcgggatc cagcgacccg aggcgcacgt
     6361 tcaggcccgc ggccgagaag atgcgtacca gctgcttgac gttcatcagg tagaacgtgt
     6421 tgcgggtgtg gttctcgggg atcagcagca ggttcttcgc ctcggggcag atcttctcga
     6481 tcgccgccat cgccgcctgg atggcgagcg gcatcatctg ctccgtgagg ttgttgaagc
     6541 cgcctgggaa caggttggtg tcgaccggcg ccagcttgta gcccgcgttg cgcacgtcca
     6601 ccgacgtgta gaacggcggc gtgtgctcca tccactccag ccggaaccag cgttcgatcg
     6661 cgggcgcgga ttccaggacg cgctgttcga gttcgttgat cgggccggtc agggcggtga
     6721 cgaggtgggg gaccatgacg ggtcgtcctt gggaggcgga agaaggattc taggtcgggg
     6781 cgtccggggc gcggccggcc aggcggcgca gttggggccg cgccccccga atgcaagcgc
     6841 ccatgaaaaa ggggccgccc tcgcgggcga ccccaagctc tcgggccggc ggcttcgacg
     6901 ccggcgccct ctctctcttc ggtcgattac ttggcgtagg ccgtctcgcc gtgcgacgtg
     6961 atgtccaggc cttcgcgctc ctcgtcttcc gtcacgcgca ggccgatcgt caggtcgacc
     7021 agcttgtagg cgacgaacga gacgacgccc gaccacgcca ccgtcacgcc gaccgccttc
     7081 gcctggatca cgaactggtc ccagatgccg ttggagccgg tggtcatcga gacccagtcg
     7141 gtgaccatgc ccgggccgcc gagcgactgg ttgttgagga tgccggtcag cagcgcgccg
     7201 acgatgccgc agacgccgtg cacgccgaac acgtccagcg tgtcgtcggc gcccagcagc
     7261 ttcttcaggc cgtgcacgcc ccacaggccc gcgaagccgg cgatgaagcc cagcacgatc
     7321 gcgcccatca ggccgacgtt gccggcggcc ggcgtgatcg ccaccagtcc ggcgacggca
     7381 cccgaggccg cgcccagcat cgaggccttg cccttcatca gcgcttcgcc gagcgaccag
     7441 gccagcaccg ccgcggccgt cgccgtgaac gtgttgacga aggccagcgt ggccgaggtg
     7501 ccggcctcga gcgccgaccc ggcgttgaag ccgaaccaac ccacccacag cagcgaggcg
     7561 ccgaccatgg tcagggtcgt cgagtgcggc gtgaacgcct ccttgccgta gccgattcgc
     7621 ttgcccacca tgtacgcgcc caccaggccc gcgacggcgg cgttgatgtg caccacggtg
     7681 ccgcccgcga agtcgagcgc gccccactgc cagatcaggc cggcggtggc gttctgcgcg
     7741 tcgacgaccg acttgctggt gtaggcgtcc ggaccttgcc agaaccagac catgtgggcg
     7801 atcggaaggt agctgaaggt gaaccacagc accatgaagg ccagtacggc ggagaacttc
     7861 atgcgctcag cgaacgagcc gacgatcagg cagcacgtga tcgccgcgaa ggtcgcctgg
     7921 aacacgacga agacgagctc cggcagcacg acgcccttgc tgaaggtcgc ggcgttggag
     7981 aacacgccct tcatcgggtc gaaggtgccc gacagcccca gtcgggagaa gccgccgaag
     8041 aagggcgaac tccagccgct gccctgcgtg aacgcgaggc tgtagccgta cagcgcccac
     8101 agcacgacga tcagcgagaa ggtgacgaac acctgcatca gcaccgacag catgttcttg
     8161 ctgcgcacga ggccgccgta gaacagtccc agcgccggga tcgacatcat gatgaccagc
     8221 gcggtcgaca ccagcatcca ggagacgtcg cccttgttga cggtctcggt cggggccgag
     8281 gccgcggcat ccggggccga tgccgccgcg gcg
//
LOCUS       DASVLN010000977        14301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_428041, whole genome shotgun sequence.
ACCESSION   DASVLN010000977 DASVLN010000000
VERSION     DASVLN010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 14301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14301
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428041"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VF453_22925"
     CDS             complement(<1..631)
                     /locus_tag="VF453_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7690576.1"
                     /translation="MARLTRLSLPGLPHHLMQIGHNRQAIFVDDEDRRAWCAHLREAS
                     LVARVDVHAYVLMDNHVHLLATPRENAEGVSTMMQSLGRRYVAAFNRRHGRTGTLWEG
                     RFRAALVDPDDSLLPCMRYIDSHAQRSGLCADPADYPWSSCAHHLGRRRDPLIVDHAR
                     YWALGNTPFEREGAFRSFLEEGVSQREAQAISQATRRGWPIAGIEGQRRL"
     gene            complement(733..1926)
                     /locus_tag="VF453_22930"
     CDS             complement(733..1926)
                     /locus_tag="VF453_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEX7690577.1"
                     /translation="MSVSPLVERPPYASDPARSRGRRRPEPAAPTRSDFQRDRDRIVH
                     STAFRRLVYKTQVFLNHEGDLFRTRMTHSLEVAQLGRSVARTMGLDEDLVEAIALAHD
                     LGHTPFGHAGQDALDDCMREHGGFEHNLQSLRVVDELELRYPEFDGLNLTLETREGIL
                     KRCTLAQAQALEASEPGGVGHRFVADLRPSLEAQLTNLADEIAYNCHDVDDGVRSGLL
                     DPEQLGAVPLYVRLRDEALAEHPRLRALPARRLVHETLRRMLSQMIYDLIDASQAALD
                     AAAPASPDAVRALPPTMRFSAAMRRESSELKRFLFANLYRHPAVNATTERAREVVRAL
                     FATYLDRPAEMSEEYESAADRPRAVADYIAGMTDRFALKEHQRLTGRVLFGDSWASLG
                     HAPAA"
     gene            complement(1948..3090)
                     /gene="aroB"
                     /locus_tag="VF453_22935"
     CDS             complement(1948..3090)
                     /gene="aroB"
                     /locus_tag="VF453_22935"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX7690578.1"
                     /translation="MQTSHAPSPASSPAATDAATVRIDLGDRSYPILIGGDLLADRAT
                     WADVGNPRAAALIVTNVTVGPLYAAVLEHAIAPHFAQVRTLALPDGESHKDWASLNLV
                     FDEMLGSGADRKTVLFALGGGVVGDMTGFAAACYMRGVPFVQVPTTLLAQVDSSVGGK
                     TAINHPLGKNMIGAFYQPQRVVCDLGVLDTLPDREMSAGLAEIIKYGACIDGEFLSWL
                     EAHVDALRARDRAALVHAVRRSCEIKAQVVGADEKEAGLRAILNFGHTFGHAIEAGLG
                     YGEWLHGEGVGCGMVMAAELSARLGLVGEAFARRIERLVERAGLPTRGPRALSPARYL
                     ELMRVDKKSERGDIRFVLLDGESRAVVRPAPEAQVTAVIAALTAAA"
     gene            complement(3097..3639)
                     /locus_tag="VF453_22940"
     CDS             complement(3097..3639)
                     /locus_tag="VF453_22940"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX7690579.1"
                     /translation="MPVRPISLIGLPGCGKTTIGRQLARVRQLPFVDADHEIERYLGC
                     SIRDFFDREGEPAFRDIEQRVIAELLDAPAPVVLATGGGAVLREETRQALRRKSTVVY
                     LRAQPDDLARRLGRDTTRPLLQGVDPRVRLRELFAQRDAFYRETAQLAVDTAHKTATT
                     LANLISMQIDMADARKEDVE"
     gene            complement(3630..5831)
                     /locus_tag="VF453_22945"
     CDS             complement(3630..5831)
                     /locus_tag="VF453_22945"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7690580.1"
                     /translation="MSLGVMLVAAVLAGCSAGVSNDGQSGSGGTGGTGGTGSVATPSM
                     TLALSSNSVTSVAPVTVSANVLDANKAPVANALVTFSTTSVGQLGSTTALTDSTGVAS
                     VQLSPASATTVGAGTVSAASSVNGTGVSATANFQTTAVSAGFGTFSTDQGTATTSRLA
                     AYAQANLTLTLTGVSAASPATVAVTSACVSAGKATISPATVSNTSGTATFVYKDTGGC
                     GATNGSDTVTATITGSSATAATLQIFLTSPTVNSITFDSTVIVGGSGSANTIYLKGSG
                     YAESAQVRFKVVDTAGNPLPGQKVDLSLSTVAGGLTLDGQSILNGRDTPEVKTSDANG
                     IVTGIVNSGTVPTPVRVIATLDNTTITTVSSNLAVLTGLPSQAHFSLAQGTINIEGGD
                     WDGTPNTYTVTASDRSGNPVPDGTSIVFWAEGGTIVGSANTALNNLGLSQATVNFQSS
                     EPRPADGRVTILAYAIGEEAFIDLNGNNVWDPGEPFQDLGDIVKDVLHDGIYDPANDE
                     FVSLAQVAVTPSSSACDNSAAASYPQLDDTNFDLAIKPGSCDGTWSGKTYVRRETETV
                     LSSSTPRPLWFAPTDTQAFNDGLPATTCRKVALRTAPVTLQPVASSYYAADAGDIWYT
                     GTTDQAGSTGNYSGTLFFTVADQNALRLNPMPAGTTISVASQTSNVAFTVLGGSPVIS
                     TSDATLTGISYTFNDKTLSSASFTVTYTTPRNVSTTFTYSLKVGARPTACQ"
     gene            complement(5874..8024)
                     /gene="pilQ"
                     /locus_tag="VF453_22950"
     CDS             complement(5874..8024)
                     /gene="pilQ"
                     /locus_tag="VF453_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX7690581.1"
                     /translation="MRSWTAGALLVLALGVALPATSWAEPKIRSVTGQQQGGTDVVRV
                     ELSEPLAAVPAGFSMQTPPRVVVDLPGVTNASGKNAVEINQGNVRSVAVASSGDRTRL
                     VLNLKSAANYRAEVQGNVLLVSLDSAGVASMSATATVPAPAAAAETTHFASSQNTDVL
                     DLRDIDFHRGADGAGRIIVNLANTQVGVDLKQQGKDLVVDFLRSTAPPSLRRRLDVSD
                     FGTPVQTITTTQVGDHVHMVVSPTGAWEHSAYQTDNQFVLEVRPAKQDANKLVPGVGY
                     TGQRLSLNFQSIEIRALLQIIADFSDFNVVTSDTVQGSVTLRLKDVPWDQALDIILQS
                     KGLDKRRTGNVLLIAPKEELAAKEQVDLEAKRKIADIEPLKTQAFQLNYTKSDDVARA
                     LTGSGSGSSAGNTTRILSARGTVMSEPRTNQLFVSDIPSKLEEVQAMISRIDVASRQV
                     MIEARIVEANDNFGRALGVKLGSTSTNAFHVGGSQYVSVGGNYNAIGSQTGQSTGGTR
                     TADYADSTFTNFGANAGAVAANASAATFALSLFSASAGRFLNMELSALEEDGTGRIIS
                     SPRVITADQVQAVVEAGEEIPYQVATSSGATSISFRKANIRLEVTPQITPEGNVILDV
                     DVNKDSRGIQTTAGFTIDTKHVKTTALVENGGTVVLGGLFQQTDTNTVDKVPLLGDIP
                     VLGNLFKTTTKISNRTELLIFITPRVITDRTVAH"
     gene            complement(8048..8581)
                     /locus_tag="VF453_22955"
     CDS             complement(8048..8581)
                     /locus_tag="VF453_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEX7690582.1"
                     /translation="MKRVLSITVGAALLAGLGACSPDNDDLIQWMEQQHKEVKPNVPP
                     VYPPKKFDPQAYVGASGVEPFGQQKLVAQTAESSQRSSALLSAAKNHVPEELESYPLD
                     SMTMVGSLRQGGKTHALLLVDGKLRDVTVGNHIGQNYGEIKSISDSEVTLVETVQDAT
                     GEWVQRTSTLQMQEKAR"
     gene            complement(8578..9204)
                     /locus_tag="VF453_22960"
     CDS             complement(8578..9204)
                     /locus_tag="VF453_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX7690583.1"
                     /translation="MEAMRGGLGQFRDLNPNEPGQWPLLPRLTVWALLAAVVVVAGYV
                     VWLSDESDSLQAERDKEPKLKEEYRGKLAQAINLDALKAQKLEVQGYVTQLEKQLPGK
                     AEMDALLTDINQAGVGRGLVIDQFVPGQTETKEYYAELPIAIRVTGRYHDIGAFAADI
                     AALSRIVTLHNLNIVPGANPGTLSMDATARTYRYLDPSEVAEQRKGKK"
     gene            complement(9253..9909)
                     /locus_tag="VF453_22965"
     CDS             complement(9253..9909)
                     /locus_tag="VF453_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX7690584.1"
                     /translation="MILINLLPHREARRQQRKQAFLAGTGFAVLFGAAIVALWYTVIS
                     TMISNQQSRNAFLQSEIKRLDAQIADVANLRSEIDALKARQKAVEDLQADRNMPVYLL
                     NDLVKQVPEGVYLTSIHQNGQQVALTGLAQTNERISEFLRNASNDTDGWLEKPILGSI
                     HAVTVTPAGSRDQRRMFDFAMGVTLKRPPPPGEAASGVAAGALGASAPRVAASAAVKG
                     "
     gene            complement(9906..10943)
                     /locus_tag="VF453_22970"
     CDS             complement(9906..10943)
                     /locus_tag="VF453_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEX7690585.1"
                     /translation="MIGLDVSSSSAKLVELGQTPSGELVLERFASEPFEKGWIADGQI
                     EKFDEVADAVRRLVAKSGAKTRDVVMAMPQSAVITKRIVLPAGLREAEMEIQVESEAH
                     QYIPFSLDEVSLDFCVIGPSASSIGDVDVLIAASRKDRVQDRQGLAEAAGLKPVVLDI
                     ESHASRLAMSRVISSLPEQGKDVLVALFEIGADTAGLKVLRGDEMLYDRDQTFGGAQL
                     TQLISRQYGFSFEEAEQKKLAGDLPEDFETTLLMPFVDSLAHEIGRALQYFFTSTPHH
                     KVDYVMLAGGTSSLAGLRERVKEVTGFACRVVNPFENMKLGSAVREGKLRREAPSYLT
                     ACGLAMRRFVQ"
     gene            11241..13631
                     /locus_tag="VF453_22975"
     CDS             11241..13631
                     /locus_tag="VF453_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEX7690586.1"
                     /translation="MSDSDLTPAKLPPAPPRETPPAHWARRAARAVLRVFLTIAGGTL
                     ALLLAVAICVGIGLAVAYPNLPDLNALTDYRPKLPMRIFSADGVLLGEYGEERRQFTP
                     IQAIPKVMRDAVLAIEDARFYHHGGVDYMGVIRAGMANVGEARSQGASTITMQVARNF
                     YLSTEKTFTRKIYEILLALKIESQLSKDQILELYMNQIYLGERAYGFASASEIYFGKP
                     LKDITIAEAAMLAGLPKAPSAYNPINNPTRAHERQQYIIDRMFVNGFITEDERKRAHA
                     QVLTYRAPVESPIHAEYVAEMARQLVFAQYGDEAYTRGLNVTVTVDSVQQLAAYHALR
                     RGIMDYERRQQFRGPEGYVDLPGDPKAVDERVSEALNEHPDNDDLRSAVVLEVSPKKV
                     VAELQSGDTITVTGDGLKPVTSGLSPKADQKVQIRRGSIVRAMSSPKGDWTLTQLPEV
                     EGAFVASEPSTGAIRALVGGFDYGKNKFNHVTQAWRQPGSSFKPFIYSGALEKGFTPS
                     TIINDAPLFFDAGTTGSQPWEPKNYDGTYDGPMTMRNALAKSKNLVSIRILQSIGPAY
                     AQQWISRFGFDPEKHPPYLTMALGSGSVTPLQMAVGYDTFATGGYRVNPMLITRITDA
                     RGKVLLDEVPKPLDESMRVIDARNAFMMESLLNSVTRIGTAARAQATLKRPDVYGKTG
                     TTNDSNDAWFCGFQPSLSAIVWIGYDTPRNLGNRETGGGLALPVWIEFMQQALKDVPV
                     AELVPPDGVTNINGEWYYNEFGPAAGVSGVGLDDKAPKETTDDEKKSILDLFKTGN"
     gene            complement(13754..14104)
                     /gene="cyaY"
                     /locus_tag="VF453_22980"
     CDS             complement(13754..14104)
                     /gene="cyaY"
                     /locus_tag="VF453_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEX7690587.1"
                     /translation="MSTPALPDSEYHAAADRLLAAIEGQCDAWLQEGVIDIDANANGG
                     LVELEFPDRSRIVVNKQPPLHEIWLAARQGGYHFRLVDGRWLDTRDGVEFFARLSQEA
                     SVQAGKPLVWQAGD"
     gene            14124..>14301
                     /locus_tag="VF453_22985"
     CDS             14124..>14301
                     /locus_tag="VF453_22985"
                     /inference="COORDINATES: protein motif:HMM:NF025012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEX7690588.1"
                     /translation="MEGSRTSSLAAARAGRSRSTPARGAALLAAALLALLAACGQKGP
                     LFLPPPERAASAPAP"
BASE COUNT         2227 a         4820 c         4921 g         2333 t
ORIGIN      
        1 ccaggcggcg ctggccctcg atcccggcga tgggccagcc gcgacgggtg gcctgggaga
       61 tcgcctgcgc ctcgcgctgg ctgacccctt cttccaggaa gctgcggaac gcaccctcgc
      121 gttcgaaggg cgtgttgccg agcgcccagt agcgcgcgtg atcgacgatg agcggatcgc
      181 gccgccggcc gagatggtgg gcgcagctcg accagggata gtccgccggg tcggcgcaca
      241 ggccgctgcg ctgggcgtgg ctgtcgatgt agcgcatgca gggcagcagc gagtcgtcgg
      301 gatcgaccag cgccgcccgg aagcgcccct cccagagcgt gcccgtgcgg ccgtggcgcc
      361 ggttgaacgc cgcgacgtag cgccggccga gcgactgcat catcgtgctg acgccctcgg
      421 cgttctcgcg tggcgtggcc agcaagtgga cgtggttgtc catcagcacg taggcatgca
      481 cgtccacgcg ggcgaccagg ctcgcctcgc gcaggtgggc gcaccaggcc cggcgatcct
      541 cgtcatcgac gaagatcgcc tgccggttgt ggccgatctg catcaggtga tgaggcagtc
      601 cgggaagcga gagacgggtc aggcgtgcca tggctgggcc gaagtgtagc tgacccccat
      661 tgttttcacc caggacgact gctcgaccat tcgggcgatc gatgccggcg ccgcgcggca
      721 gtggcgacgc ggtcaggcgg ccggcgcgtg cccgagcgag gcccacgagt cgccgaacag
      781 cacgcggccg gtgagccgct ggtgctcctt gagcgcgaag cggtcggtca tcccggcgat
      841 gtagtcggcc acggcacgcg ggcggtcggc ggcggattcg tactcctcgc tcatctcggc
      901 gggacggtcg aggtaggtcg cgaacagtgc ccgcacgacc tcccgggcac gttcggtcgt
      961 cgcgttgact gccggatggc ggtacaggtt cgcgaacagg aagcgcttga gctcgctgct
     1021 ctcgcggcgc atcgcagcgc tgaagcgcat cgtcggcggc agcgcgcgca ccgcatccgg
     1081 gctggccggg gccgcggcgt cgagcgccgc ctggctcgcg tcgatcaggt cgtagatcat
     1141 ctgcgagagc atgcgccgca gggtctcgtg caccaggcgg cgcgccggca gcgcacgcag
     1201 gcgcgggtgc tcggcgagcg cttcgtcgcg cagccggacg tacagcggca cggcccccag
     1261 ctgctccgga tcgagcaggc cggagcgcac gccgtcgtcg acgtcgtggc agttgtaggc
     1321 gatctcgtcg gcgaggttgg tcagctgtgc ctccaggctg ggccgcaggt cggcgacgaa
     1381 gcgatggccg acgccgccgg gctcgctcgc ctcgagcgcc tgcgcctggg ccagcgtgca
     1441 gcgcttgagg atgccttcgc gcgtctccag cgtcaggttc aggccgtcga actcggggta
     1501 gcgcagctcc agctcgtcga ccacgcgcag gctctggagg ttgtgctcga agccgccgtg
     1561 ttcgcgcatg cagtcgtcga gcgcatcctg gccggcgtgg ccgaagggcg tgtggccgag
     1621 gtcgtgcgcc agggcgatgg cctcgaccag gtcctcgtcc agccccatcg tgcgcgccac
     1681 cgagcgcccg agctgcgcca cctccagcga gtgcgtcatg cgggtgcgga acaggtcgcc
     1741 ctcgtggttc aggaagacct gggtcttgta gacgaggcgc cggaacgccg tgctgtggac
     1801 gatgcggtcg cggtcccgct ggaagtccga gcgcgtgggc gccgcgggct ccgggcggcg
     1861 gcggccgcgg ctgcgcgccg gatcgctggc gtagggcggg cgctcgacga ggggagagac
     1921 gctcacgccg atgcccgtcg acccaggtca ggccgccgcg gtcagcgcgg caatcaccgc
     1981 cgtcacctgc gcctcgggcg ccgggcggac gacggcgcgg ctctcgccgt ccaggaggac
     2041 gaagcggatg tcgccccgct cggacttctt gtcgacgcgc atcagctcca ggtagcgcgc
     2101 cggcgacagc gcgcgcggcc cgcgggtcgg caggcccgcg cgctcgacga ggcgctcgat
     2161 gcggcgagcg aacgcttcgc cgacgagccc gagacgcgcc gacagctccg cggccatcac
     2221 catgccgcag ccgacgcctt cgccgtgcag ccactcgccg tagcccaggc cggcctcgat
     2281 ggcgtgaccg aaggtgtggc cgaaattcag gatggcgcgc agtccggcct ccttctcgtc
     2341 ggcacccacg acctgggcct tgatctcgca cgaacggcgc accgcgtgca cgagggccgc
     2401 gcgatcgcgc gcgcgcaggg cgtcgacgtg cgcctcgagc caggacagga actcgccgtc
     2461 gatgcaggcg ccgtacttga tgatctcggc gaggcccgcg gacatctcgc ggtccggcag
     2521 cgtgtccagc acgcccaggt cgcagaccac gcgctgcggc tggtagaagg cgccgatcat
     2581 gttcttgccg agcgggtggt tgatcgccgt cttgccgcca accgacgagt ccacttgcgc
     2641 gagcagcgtc gtcggcacct gcacgaacgg cacgccccgc atgtagcagg cggccgcgaa
     2701 gcccgtcatg tcgcccacga cgccgccgcc cagcgcgaac agcaccgtct tgcgatcggc
     2761 gcccgagccg agcatctcgt cgaagaccag gttgaggctc gcccagtcct tgtggctctc
     2821 cccgtcaggc agggccagcg tgcgcacctg tgcgaagtgc ggcgcgatcg catgctcgag
     2881 caccgccgcg tacagcgggc cgacggtcac gttggtgacg atcagcgccg ccgcgcgcgg
     2941 attgccgacg tcggcccagg tcgcgcgatc cgccaggagg tcgccgccga tcaggatggg
     3001 gtagctgcgg tcgccgaggt cgatccggac ggtggccgca tcggtcgcgg ccggcgagga
     3061 ggccggggag ggcgcgtgag aggtctgcat cgaccgctac tctacgtcct ctttgcgggc
     3121 atccgccatg tcgatctgca tcgagatcag gttggcaagg gtcgtcgcgg tcttgtgggc
     3181 ggtgtccacg gccagctgcg cggtctcgcg gtagaaggca tcgcgctgcg cgaagagctc
     3241 gcgcagccgc acgcgaggat cgacgccctg cagcagcggg cgcgtggtgt cgcgtccgag
     3301 acggcgcgcg aggtcgtccg gctgcgcgcg caagtagacg accgtggact tgcgccgcag
     3361 cgcctggcgg gtctcctcgc gcagcacggc gcctccgccc gttgcaagca cgacaggcgc
     3421 gggcgcgtcg agaagctcgg cgatcacgcg ctgctcgatg tcacggaagg ctggctcgcc
     3481 ttcgcgatcg aagaagtcgc ggatcgagca acccaggtag cgctcgatct cgtggtcggc
     3541 gtccacaaac ggcaactggc gcacgcgcgc cagttgtcgc ccgatcgtgg tcttgccaca
     3601 gccgggcagg ccgatgaggg agatcggcct tactggcacg ccgtcgggcg ggcgccgacc
     3661 ttcagcgagt aggtgaacgt cgtcgacacg ttgcgtggcg ttgtgtaggt cacggtgaag
     3721 ctagccgagc tcagcgtctt gtcgttgaag gtgtaggaga tgcccgtcag ggtggcatcc
     3781 gaggtgctga tcacggggga gccgcccagc acggtgaagg ccacgttcga cgtctgcgac
     3841 gccaccgaga tcgtcgtgcc ggccggcatc gggttcaggc ggagggcatt ctggtccgcg
     3901 acggtgaaga acagggtgcc ggaatagttc ccggtgctgc cggcctggtc agtcgtgccg
     3961 gtgtaccaga tgtcgccggc atccgccgcg tagtagctgg acgccaccgg ctgcagcgtg
     4021 acgggcgcgg tccgcaacgc gaccttgcgg catgtggtcg ccgggaggcc gtcgttgaag
     4081 gcctgcgtgt cggtcggcgc aaaccacagc ggccgcgggg tcgacgaaga caagacggtc
     4141 tcggtctcgc gccgcacgta ggtcttgccg gaccacgtgc cgtcgcacga gcccggcttg
     4201 atcgcgagat cgaagttcgt gtcgtccaac tgcggatagc tggccgcagc gctgttgtcg
     4261 caggcgctgg agctcggcgt gaccgcgact tgcgcgagcg agacgaattc gtcgttggcg
     4321 gggtcgtaga tgccgtcatg gagcacgtcc ttgacgatgt ccccgagatc ctggaacggc
     4381 tcgcccggat cccagacgtt gttcccgttc aagtcgataa acgcctcctc gccgatcgcg
     4441 taagcaagaa tcgtcacccg gccgtcggcg gggcgcggct cagatgactg gaagttgacg
     4501 gtggcctgcg acaagcccag gttgttcaac gcggtgttgg cggagcctac gatggtgccg
     4561 ccctcggccc agaacacaat ggacgtcccg tcgggcaccg ggttgccgct acgatcggag
     4621 gcggtcaccg tgtaggtgtt gggcgtgccg tcccagtcgc caccctcgat gttgatcgtg
     4681 ccttgggcca gcgaaaaatg cgcttgcgag ggcagtcccg tcaacacagc aagattgctg
     4741 gacaccgtcg tgatggtggt gttgtccagc gtggcgatca cgcgcacagg cgtaggcacg
     4801 gtccccgaat tcacgatgcc cgtaacgatg ccgttggcgt cgctcgtctt cacctcgggg
     4861 gtgtcgcggc cgttcaggat gctctggccg tccagcgtca agccaccggc gaccgtcgac
     4921 aaggagagat cgaccttttg cccagggagc ggattgccgg cggtgtcgac gaccttgaag
     4981 cgcacctgcg cggattcggc atagcccgaa cccttgaggt agatcgtgtt ggccgagccc
     5041 gagccaccga caatgaccgt cgagtcgaac gtgatgctgt tgaccgtcgg cgacgtcagg
     5101 aatatctgca gcgtcgccgc ggtagcggac gacccggtga tcgtcgcggt gacggtgtca
     5161 gagccgttcg tggcaccgca cccccccgtg tccttgtaca cgaacgtagc ggtacccgag
     5221 gtattcgaca cggtggcggg cgagatggtc gccttgcccg cagacacgca cgcggaggtg
     5281 acggctacgg tcgcaggcga agcggccgaa acgcccgtca gcgtgagcgt gaggttggcc
     5341 tgcgcatagg ccgcgagccg ggacgtggtg gcggtgccct ggtcagtcga gaaggtgccg
     5401 aaaccggcgc tcacggccgt ggtctggaag ttcgccgtcg cgctgacgcc agtgccgttg
     5461 accgaggaag ccgctgacac cgtgcccgcg ccaacggtgg tagcagaagc cggagacagc
     5521 tggacggatg ctacgcccgt cgagtccgtc agcgcggtag tcgaaccgag ctgtcccacc
     5581 gaggtggttg agaacgtgac cagggcgttg gcaaccgggg ccttgttcgc gtccagcaca
     5641 ttcgcggaga cggtgaccgg cgctaccgaa gtcacggagt tggacgacaa cgcgagcgtc
     5701 atgctgggcg tcgctaccga cccggttccc ccagttcccc cggttccccc gctgccgctc
     5761 tggccgtcat tgctgacacc cgcgctgcaa cccgccagta ccgccgccac gagcatcacg
     5821 cccaggctca tccatttcct catgttcata gctcccggca accttcgtgc atatcagtgc
     5881 gccaccgttc ggtcggtgat cacacggggg gtgataaaga tcagcaattc agtacggttc
     5941 gagattttcg tcgtcgtctt gaacagattg cccagcaccg ggatgtcgcc cagcagcggc
     6001 accttgtcga ccgtgttcgt gtcggtctgc tggaacaggc cgcccagcac caccgtgccg
     6061 ccgttctcga ccagcgcggt ggtcttcacg tgcttggtgt cgatcgtgaa gccggccgtc
     6121 gtctggatgc cgcggctgtc cttgttgacg tcgacgtcca ggatgacgtt gccttccggc
     6181 gtgatctgcg gcgtgacctc gaggcggatg ttggccttgc ggaacgagat cgacgtcgcg
     6241 ccgctggagg tcgccacctg gtacgggatc tcctcgccgg cctcgaccac ggcctgcacc
     6301 tggtcggcgg tgatgacgcg cgggctcgag atgatgcggc ccgtgccgtc ctcctccagc
     6361 gccgacagct ccatgttcag gaagcggccc gccgaggcgc tgaacagcga cagggcgaag
     6421 gtcgcggcgg acgcgttggc cgcgacggcg cccgcattgg cgccgaagtt cgtgaacgtc
     6481 gagtcggcgt agtccgccgt acgcgtgccg ccggtgctct ggcctgtctg cgagccgatc
     6541 gcgttgtaat tgccgccgac cgacacgtac tggctgccgc cgacgtggaa ggcgttcgtg
     6601 gacgtcgagc cgagcttgac gcccagcgcg cggccgaagt tgtcgttcgc ttcgacgatg
     6661 cgtgcctcga tcatcacctg gcgcgacgcg acgtcgatcc ggctgatcat cgcctggact
     6721 tcctcgagct tgctcgggat gtcggagacg aacagctggt tcgtgcgcgg ctcggacatc
     6781 accgtgccac gagcagacag gatgcgcgtc gtgttgccag cggagctgcc gctaccggac
     6841 cccgtgagtg cgcgcgcgac gtcgtcggac ttggtgtagt tcagctggaa ggcctgcgtc
     6901 ttgagcggct cgatgtcggc gatcttgcgc ttggcctcga ggtcgacctg ctccttcgcg
     6961 gccagctctt ccttgggcgc gatcagcagc acgttgcccg tgcggcgctt gtccagcccc
     7021 ttgctctgga gaatgatgtc cagcgcctgg tcccagggca cgtccttcag gcgcagcgtg
     7081 acgctgccct gcaccgtgtc gctcgtcacg acgttgaagt cgctgaagtc cgcgatgatc
     7141 tgcagcagcg cgcggatctc gatgctctgg aagttgagcg acaggcgctg tccggtgtag
     7201 cccacgcccg gcacgagctt gttcgcgtcc tgcttggccg gacgcacctc gagcacgaac
     7261 tggttgtcgg tctggtaggc gctgtgttcc caggcaccgg tcggcgagac caccatgtgc
     7321 acgtggtcgc ccacctgcgt cgtcgtgata gtctggaccg gcgtgccgaa gtcactcacg
     7381 tcgaggcggc ggcgcaggct cggcggcgcg gtcgagcgca ggaagtcgac gacgaggtcc
     7441 ttgccctgct gcttgaggtc gacgcccacc tgcgtgttcg cgaggttgac gatgatccgg
     7501 ccggcaccgt ccgcgccgcg gtggaagtcg atgtcgcgca ggtcgagcac gtcggtgttc
     7561 tggctgctcg cgaagtgcgt cgtctccgcg gccgccgcgg gcgcgggcac ggtggcggtc
     7621 gccgacatgg aggcgacgcc ggcggaatcg agcgacacga gcagcacgtt gccctgcacc
     7681 tccgcgcggt agttcgccgc ggacttcagg ttcagcacga ggcgggtgcg gtcacccgag
     7741 ctcgcgacgg cgaccgagcg cacgttgccc tgattgatct cgaccgcgtt cttgcccgag
     7801 gcattcgtta ccccgggcag gtcgacgacc acgcgtggcg gcgtctgcat cgagaagccc
     7861 gccggcacgg cggccagcgg ctcgctgagc tcgacgcgca cgacgtccgt gccgccttgc
     7921 tgctggcccg tcaccgaacg aatcttgggc tccgcccagg acgtcgccgg caacgccacc
     7981 cccaatgcaa gaacgagcaa tgctcccgcg gtccaactcc gcatcttccg attctcctga
     8041 ctgctgctca tcgcgccttc tcctgcatct ggagggtgct ggtgcgctgt acccactcac
     8101 ccgtggcatc ctgtacggtc tcgaccagcg tgacttcgct gtcgctgatc gacttgatct
     8161 caccgtagtt ctgaccgatg tggttgccca cggtcacatc gcgcaacttg ccgtccacca
     8221 gcagcagcgc atgcgtcttg ccgccctgcc gcaggctgcc gaccatcgtc atgctgtcca
     8281 gcggatagga ctcgagctcc tccggcacgt ggttcttggc cgcggagagc agcgcactcg
     8341 atcgctgcga cgactcggcg gtctgcgcca ccagcttctg ctggccgaac ggctccacgc
     8401 cgctcgcgcc gacgtaggct tgcgggtcga acttcttcgg cggatagacc ggcggaacgt
     8461 tcggcttgac ttccttgtgc tgctgctcca tccactggat aaggtcgtcg ttgtccggcg
     8521 agcacgcgcc gaggccggcc agcagggccg cgccgaccgt gatgctgagg acgcgcttca
     8581 tttcttgccc ttgcgttgct cggcgacctc ggacgggtcg aggtagcgat aggtgcgcgc
     8641 cgtcgcgtcc atcgacagcg tcccggggtt cgcgcccggc acgatgttga ggttgtgcag
     8701 cgtcacgatg cgcgagagcg ccgcgatgtc cgccgcgaag gcgccgatgt cgtggtagcg
     8761 gccggtcacg cggatcgcga tcgggagctc ggcgtagtac tccttggtct cggtctggcc
     8821 cggcacgaac tggtcgatca cgaggccgcg gcccacgccc gcctggttga tgtcggtcag
     8881 cagcgcgtcc atctccgcct tgccgggcag ctgcttctcg agctgcgtga cgtagccctg
     8941 cacctcgagc ttctgggcct tcagcgcatc gaggttgatc gcctgggcga gcttgccgcg
     9001 gtactcttcc ttgagcttgg gctccttgtc gcgctccgcc tgcagcgagt ccgactcgtc
     9061 gctgagccag acgacgtagc cggccacgac cacgacggcg gcgagcagcg cccacacggt
     9121 cagccgaggc agcaggggcc actggcccgg ctcgttggga ttgaggtcgc ggaactggcc
     9181 gaggccgccc cgcatcgcct ccatgagatc ggcgccgacg tcgcgcttgg ctttctgttg
     9241 cgtggccatg ccttatccct tcaccgcggc ggaggcggcg acgcgaggtg cgctggcccc
     9301 gagtgcaccc gcggcaacgc cgctggcggc ctcgcccggc ggcggcgggc gcttgagcgt
     9361 gacgcccatc gcgaaatcga acatgcggcg ctggtcgcgc gagcccgccg gcgtgaccgt
     9421 cacggcgtgg atggaaccga ggatgggctt ctccagccag ccgtccgtgt cgttgctcgc
     9481 gttgcgcagg aactccgaga tgcgctcgtt ggtctgcgcg agacccgtca gcgcgacctg
     9541 ctggccgttc tgatggatcg aagtcaggta gacgccctcc ggcacctgct tgaccaggtc
     9601 gttgagcagg tacacgggca tgttgcggtc ggcctgcagg tcctccaccg ccttctggcg
     9661 cgccttcagg gcgtcgatct cgctgcgcag gttggcgacg tcggcgatct gcgcatccag
     9721 acgcttgatc tcggactgca ggaacgcgtt gcgcgactgc tggttgctga tcatcgtgga
     9781 gatgaccgtg taccagagcg cgacgatggc ggcgccgaac aggacggcga aaccggtccc
     9841 tgccaggaag gcctgcttgc gttgctggcg ccgcgcttcg cggtgcggca acaggttgat
     9901 caggatcact gcacgaacct ccgcatcgcc agcccgcacg cggtcaggta cgagggcgcc
     9961 tcgcgccgca gcttgccttc gcgcacggcg ctccccagct tcatgttctc gaacggattg
    10021 acgacgcggc acgcgaagcc ggtgacttcc ttgacccgtt cgcgcagccc cgctagcgac
    10081 gaagtgccgc ccgcgagcat cacgtagtcg accttgtggt gcggcgtgct cgtgaagaag
    10141 tactgcagcg cgcggccgat ctcgtgcgcg aggctgtcga cgaagggcat cagcagcgtg
    10201 gtctcgaagt cctccggcag gtcgccggcg agcttcttct gctcggcctc ctcgaacgag
    10261 aagccgtact ggcgcgagat cagctgcgtc agctgcgcgc cgccgaaggt ctggtcgcgg
    10321 tcgtagagca tctcgtcgcc gcgcaggacc ttcaggccgg ccgtgtcggc accgatctcg
    10381 aacagcgcga cgagcacgtc cttgccctgc tccggcaggc tggagatcac gcggctcatc
    10441 gcgaggcgcg aggcgtggga ctcgatgtcg agcacgaccg gcttcagccc ggccgcctcg
    10501 gccagcccct gacggtcctg cacgcgatcc ttgcgcgacg cggcgatcaa cacgtccacg
    10561 tcgccgatcg acgacgcgct ggggccgatg acgcagaagt cgaggctgac ctcgtcgagc
    10621 gagaagggaa tgtactgatg ggcctcggac tcgacctgga tctccatctc ggcctcgcgc
    10681 agcccggcgg gcaggacgat gcgcttggtg atcacggccg actgcggcat cgccatgacg
    10741 acgtcgcgcg tcttggcgcc gctcttggcg accaggcggc gcaccgcgtc ggcgacctcg
    10801 tcgaacttct cgatctggcc gtcggcgatc caacccttct cgaacggttc gctggcgaac
    10861 cgctctagca ccagttcacc cgaaggggtc tggcccagct cgaccagctt ggcgctggac
    10921 gagctgacat ccagcccgat catgggcgga tgtgttcggc cgaagaggcg atcgaggaaa
    10981 gacacgagga catgctccga cagcgctcga gacggagcgc cgggttgata ttttgcggca
    11041 atgctatcag caagaacgac aagaaaacaa agttggtgtg cgggaatgcc cgcattttca
    11101 agtcatttga cgcgcacgac cggggttcaa atccgaagga cctgcaaata cccggcggcg
    11161 agcgccgccc acaccgtcgc ccggggccgc gcagtcacgt ccggtttcta taatcgccgt
    11221 cacacgcctg ccccacattc atgagcgatt ccgacctgac gcccgcgaaa ctgccccccg
    11281 ccccgccgcg ggagacgccc cccgcccact gggccagacg cgcggcgcgg gccgtgctcc
    11341 gtgtctttct gacaattgcg ggcggcacat tagcgctgtt actcgccgtt gcaatctgcg
    11401 tcggcatcgg actcgcggtc gcgtatccca acctgcccga cctgaacgcg ctcaccgatt
    11461 accggccaaa attaccgatg cggattttct ccgcggatgg cgtgctcctt ggcgaatatg
    11521 gcgaggaaag acgccagttc accccgatcc aggcgatccc caaggtgatg cgcgacgccg
    11581 tcctcgcgat cgaggacgcg cgcttctacc accacggcgg ggtcgactac atgggcgtga
    11641 tccgcgcggg catggccaac gtcggcgagg cgcgcagcca gggcgcctcg acgatcacga
    11701 tgcaggtggc gcgcaacttc tacctgtcca ccgaaaagac attcacgcgc aagatctacg
    11761 agattctgct ggcgctcaag atcgagtccc aattgagcaa ggaccagatt ctggagctgt
    11821 acatgaacca gatttacctg ggggaacggg cctatggatt cgcctccgcc agcgaaatct
    11881 acttcgggaa accccttaaa gacatcacga tcgcggaggc ggcgatgctc gccggcctgc
    11941 ccaaggcgcc gagcgcctac aacccgatca acaacccgac gcgggcgcac gagcggcagc
    12001 aatacatcat cgaccgcatg ttcgtgaatg gattcatcac cgaggacgag cgcaagcgcg
    12061 cccacgccca ggtcctcaca taccgggcgc cggtcgagtc gccgatccac gcggaatacg
    12121 tggccgagat ggcgcgccag ctcgtcttcg cgcaatacgg cgacgaggcc tacacgcggg
    12181 ggctgaacgt gaccgtcacg gtcgactccg tccagcagct ggcggcctac cacgccttgc
    12241 ggcgcgggat catggactac gagcggcgcc agcaattccg gggcccggaa ggctacgtcg
    12301 acctgcccgg cgatcccaag gcggtggacg agcgcgtctc cgaggcgctc aacgagcatc
    12361 ccgacaacga cgacctgcga tcggccgtgg tgctcgaggt ctcgcccaag aaggtcgtcg
    12421 cggagctcca gtcgggcgac acgatcacgg tcaccggcga cggactgaag ccggtcacct
    12481 cgggcctgtc gcccaaggcc gaccagaagg tgcagatccg ccgcggctcc atcgtgcgtg
    12541 cgatgtcctc acccaagggc gactggacgc tgacgcagct gcccgaggtc gagggggcgt
    12601 tcgtcgcgag cgagccgtcg acgggcgcga tccgcgcgct cgtgggcggc ttcgactacg
    12661 gcaagaacaa gttcaaccac gtcacgcagg cctggcgcca gccgggctcg agcttcaagc
    12721 ccttcatcta ttccggcgcg ctcgagaagg gcttcacgcc ctcgaccatc atcaacgacg
    12781 ccccgctgtt cttcgacgcc ggcaccacgg gcagccagcc gtgggagccg aagaactacg
    12841 acggcaccta cgacggcccg atgacgatgc gcaatgcgct cgccaagtcg aagaacctgg
    12901 tctcgatccg catcctgcag tcgatcgggc cggcctacgc gcagcaatgg atctcgcgct
    12961 tcggcttcga ccccgagaag catccgccct atctcacgat ggcgctcggc tcgggctcgg
    13021 tgactccgct gcagatggcg gtcggctacg acaccttcgc caccggcggc taccgcgtca
    13081 atccgatgct gatcacccgc atcaccgacg cgcgcggcaa ggtgctcctc gacgaggtgc
    13141 ccaagccgct ggacgagtcg atgcgcgtga tcgacgcgcg caacgcgttc atgatggagt
    13201 cgctgctgaa ctcggtgacc cgcatcggca ccgcggcgcg cgcgcaggcc acgctcaagc
    13261 gcccggacgt ctacggcaag acgggcacga ccaacgactc caacgacgcc tggttctgcg
    13321 gcttccagcc ctcgctgtcg gcgatcgtgt ggatcggcta cgacacgccg cgcaacctcg
    13381 gcaaccgtga gaccggcggc gggctcgccc tgccggtgtg gatcgagttc atgcagcagg
    13441 cgctcaagga cgtgccggtg gccgagctcg tcccgccgga tggcgtgacc aacatcaacg
    13501 gcgagtggta ctacaacgag ttcggcccgg ctgcgggtgt cagcggcgtg ggcctcgacg
    13561 acaaggcgcc caaggagacg accgacgacg agaagaagtc gatcctcgac ctgttcaaga
    13621 ccggcaactg agccacgcgg cgcgcggcgg gttcctacag aagagcaaca ccgcccgcaa
    13681 aagtcaacgt cggaacgtaa cagtcgggct ggcgtctgga tccgccgaca gcccgtgccc
    13741 gccgggcgcg atttcagtcg ccggcctgcc agacgagcgg cttgccggcc tgcacgctcg
    13801 cctcctgcga cagccgcgcg aagaactcga cgccgtcgcg cgtgtccagc cagcggccgt
    13861 cgacgagacg gaagtgatag ccgccctggc gtgccgcgag ccagatctcg tgcagcggcg
    13921 gctgcttgtt gacgacgatc cggctgcggt ccgggaactc cagctccacc aggccgccgt
    13981 tggcgttggc gtcgatgtcg atcacgccct cctgcagcca ggcatcgcac tggccctcta
    14041 tggcggccag caggcggtcg gcggcggcgt ggtactcgga gtccgggagg gcaggcgtcg
    14101 acatggtcga tagaattcgg aggatggaag gaagccggac ttcgagttta gcggcggccc
    14161 gcgcgggccg atcccgcagc acccctgcgc gcggcgcagc cttgcttgcc gcggccctgc
    14221 tggcgctgct ggcggcatgc gggcagaagg gcccgctgtt cctgccgccg ccggagcgcg
    14281 cggcctcggc gcctgcaccc g
//
LOCUS       DASVLN010000978         3615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_374178, whole genome shotgun sequence.
ACCESSION   DASVLN010000978 DASVLN010000000
VERSION     DASVLN010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374178"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1580
                     /locus_tag="VF453_22990"
     CDS             96..1580
                     /locus_tag="VF453_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7690589.1"
                     /translation="MNAPERLPVPPELDTQALAAFSETAWKERILPQLVDYVAIPAKS
                     PLYDPDWEKHGLIDKVVRDAAQWVEGRKVPGLTLEVIRQPGRTPVIFFEVESTRPAVA
                     GTPPTIVMYGHLDKQPEFSGWRNDLGPWTPKIEGDKLYGRGGADDGYAVYAAVSAIEA
                     LKAQGATHPRIVGLIESCEESGSFDLPVYLDLLKDRLGDVSLVVCLDSGAGNYDQLWL
                     TSSLRGNVTGTLKVEILTEGIHSGDASGLVPSSFRVLRHVLDRLEDSATGHLLPESFH
                     CQVPADRLEQAKVAASILGEEVWKRIPWACGGDGGPVLPTTTDPVEALLRRTWRPTLS
                     VTGVDGFPDIRNAGNVLRPFSAFKLSLRTPPLVDANEVAEKLKHLLEDNAPYNAKVTF
                     EADGRAGAYGATGWNAPPLDAWMESALDAASRAQFGAPLAYIGQGGTIPLMNLLQQGF
                     PKAQMMVCGVLGPKSNAHGPNEFLHLPYARRLTAAVAQVIAAHP"
     gene            complement(1629..1844)
                     /locus_tag="VF453_22995"
     CDS             complement(1629..1844)
                     /locus_tag="VF453_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690590.1"
                     /translation="MIEIPDGWASMLVSAVRDAVLYQEQLLTSQTLRNREDYEEHLMH
                     LSQFFEFVKEQYAKLDPSGRIPLDKLL"
     gene            complement(1841..2305)
                     /locus_tag="VF453_23000"
     CDS             complement(1841..2305)
                     /locus_tag="VF453_23000"
                     /inference="COORDINATES: protein motif:HMM:NF037873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690591.1"
                     /translation="MSIVILKDLLLEQGQIDRYYEGSVPVDLWRALKKTQKSGIFDFV
                     EAEYTLSNGRPRPADITIVEKRGEKWVEIKQRPRGVSTFDALGAPPGDGWNYYRIPAG
                     TVLPAGLCVVRDEFNPRYKATHHTIAPAYDMPLDTFKKLLGELAASVIKEAA"
     gene            complement(2401..2853)
                     /locus_tag="VF453_23005"
     CDS             complement(2401..2853)
                     /locus_tag="VF453_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEX7690592.1"
                     /translation="MTAAELQAYLHGHIPLSAAMQVAVDEADIGRVRLRAPLAPNINH
                     RETVFGGSASAVAILAAWSLLHLRLRADGLSSRLVIQRNTMDYGLPILGEFTATASLA
                     APDAWPRFLRTLARMGKARAEVQAVLEADGKVVGRLTGEFVALSSEQS"
     gene            complement(2853..3065)
                     /locus_tag="VF453_23010"
     CDS             complement(2853..3065)
                     /locus_tag="VF453_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690593.1"
                     /translation="MRIVGILLVLAGVVALATGGFTYTQDKTVAKLGPLELNSKETHT
                     VDFPLWGGVGAIVAGVALVAFGSRKR"
     gene            3317..>3615
                     /locus_tag="VF453_23015"
     CDS             3317..>3615
                     /locus_tag="VF453_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690594.1"
                     /translation="MAPPSFDSRAAAERWHRFTAATVRAFHRYGTWLVSLSWWRFGWY
                     SLLLLVASGVIQKLPPFSWTYTETVTESARHRHARTAPTPPAAPSFAAPFVPPVPP"
BASE COUNT          557 a         1304 c         1217 g          537 t
ORIGIN      
        1 taccccagaa cctgccgccg gccgggcgcc gccggggcgc gccacccgtg cgcccgagga
       61 acgccgcggc ccgcgcgcga cccacggagc ccgccatgaa cgcccctgaa cgcctgcccg
      121 tcccgcccga gctggatacg caagccctcg ccgccttctc ggagacggcg tggaaagaac
      181 gcatcctgcc gcagctggtg gactacgtcg cgatcccggc caagagcccg ctgtacgacc
      241 ccgactggga aaagcacggc ctgatcgaca aggtcgtgcg cgacgcggcc caatgggtcg
      301 agggccgcaa ggtgccgggc ctgacgctgg aggtgatccg ccagcccggc cgcacgccgg
      361 tgatcttctt cgaggtcgag tcgacgcgcc ccgccgtcgc cggcacgccg ccgaccatcg
      421 tgatgtacgg ccacctcgac aagcagcccg agttcagcgg ctggcgcaac gacctcggcc
      481 cgtggacgcc caagatcgag ggcgacaagc tctacggccg cggcggcgcg gacgacggct
      541 acgcggtcta cgccgcggtc agcgcgatcg aggcgctcaa ggcgcagggc gcgacgcacc
      601 cgcgcatcgt gggcctgatc gagagctgcg aggagtcggg ctcgttcgac ctgccggtct
      661 acctcgacct gctcaaggac cggctgggcg acgtctccct ggtcgtctgc ctcgactcgg
      721 gcgccggcaa ctacgaccag ctctggctga cgagcagcct gcgcggcaac gtgacgggca
      781 cgctgaaggt cgagatactg accgagggca tccactccgg tgacgcgagc ggcctcgtgc
      841 cctcgagctt ccgcgtgctg cgccatgtgc tcgatcgcct cgaggactcg gccaccggcc
      901 acctgctgcc ggagagcttc cactgccagg tgccggccga ccggctcgag caggcgaagg
      961 tcgcggcgtc catcctcggc gaggaggtct ggaagcgcat cccctgggcc tgcggcggcg
     1021 acggcggccc ggtgctgccc acgacgaccg acccggtcga ggcgctgctg cgccgcacct
     1081 ggcggccgac gctgtcggtc acgggcgtcg acggcttccc cgacatccgc aacgccggca
     1141 acgtgctgcg cccgttcagc gccttcaagc tgagcctgcg cacgccaccg ctggtcgatg
     1201 ccaacgaggt ggcggaaaag ctcaagcacc ttctcgagga caacgcgccc tacaacgcca
     1261 aggtcacctt cgaggcggac ggccgcgccg gcgcgtacgg cgcgaccggc tggaacgcgc
     1321 cgccgctgga cgcgtggatg gaatcggccc tggacgccgc cagccgcgcc cagttcggcg
     1381 cgccgctcgc ctacatcggc cagggcggca cgatcccgct gatgaatctg ctgcagcagg
     1441 gcttccccaa ggcgcagatg atggtctgcg gcgtcctcgg ccccaagagc aacgcccacg
     1501 gccccaacga gttcctgcac ctgccctacg cccgccgcct gaccgccgcg gtggcgcagg
     1561 tgatcgcggc gcatccctga gcggccgttc acgtagcccg cgagcgcttc gcgggccacg
     1621 ggcgtcgctc agagaagctt gtcgagcggg atgcgtcccg atggatccag cttggcgtac
     1681 tgctccttca cgaattcgaa gaactgcgag agatgcatca gatgctcctc gtagtcttcg
     1741 cgattgcgca gcgtctggct ggtgagcaac tgctcctgat agagcactgc atcacgcacc
     1801 gcagacacga gcatcgacgc ccagccgtcg gggatctcga tcacgccgcc tccttgatca
     1861 ccgatgcagc gagctcgccg aggagcttct tgaaagtgtc gagcggcatg tcgtaggcgg
     1921 gtgcaatggt gtggtgtgtt gccttgtatc gcgggttgaa ctcatcacgg acgacgcaca
     1981 agcctgcagg tagcaccgtg ccggccggaa tgcggtagta gttccatccg tctccgggcg
     2041 gggcgccgag cgcgtcgaag gtactgacgc cacgcggacg ctgtttgatc tcgacccact
     2101 tttcgccccg cttttcgacg atcgtgatgt ccgctgggcg cggacgtccg ttgctcagcg
     2161 tgtattccgc ttccacaaaa tcgaagatgc ccgatttctg cgtcttcttg agcgcacgcc
     2221 agagatcgac cggcacactg ccttcgtagt accggtcaat ctgcccctgc tccaggagca
     2281 ggtccttcaa aatcacgatc gacattgctg atttccccct gacaagctcg tacgagtgtg
     2341 gggattctcc gcgcgaggat cggagcaagc catccccaaa ctgggagcta ttacgtgtcc
     2401 ttacgactgt tcgctcgaca gagcgacgaa ctcgccggtc agccgaccga cgaccttccc
     2461 gtccgcctcg agcacggcct gcacctccgc gcgggccttg cccatgcgcg cgagcgtgcg
     2521 caggaagcgc ggccaggcgt cgggggctgc gagcgacgcg gtggccgtga actcgcccag
     2581 gatgggcagc ccgtagtcca tcgtgttgcg ctggatcacg aggcggctcg agaggccgtc
     2641 ggcgcgcagc cgcaggtgca gcagcgacca ggcggcgagg atcgcgaccg ccgacgcgct
     2701 gccgccgaac accgtctcgc ggtggttgat gttgggcgcc agcggcgcgc gcaggcgcac
     2761 gcgtccgatg tcggcctcgt cgacggccac ctgcatcgcc gccgacagcg ggatgtgccc
     2821 gtgcaggtag gcctgcagct cggccgccgt catcagcgct tgcggctgcc gaacgccacc
     2881 agcgcgacgc ccgcgacgat cgcgccgacg ccgccccaga gcgggaagtc cacggtgtgc
     2941 gtttccttgg agttgagctc gagggggccg agcttggcga cggtcttgtc ctgggtgtag
     3001 gtgaagccgc ccgtggcgag cgcgacgacg ccggccagga cgaggaggat gcccacgatg
     3061 cgcatgcgag ttccttcgcg aggagcggcc cgcggccgcg acgacgcaag gttagcgcga
     3121 ccgtggcgcg gcgggcgtcg gacgagggcg cgtcgctctc gacggcccgt ggcaggcgcg
     3181 cgccgcggcc ggtccgtccg gcaccgctgc agtctgtccc cccgaatgcc gcgccaatcg
     3241 cgccgcactg ccgggccggg ccgcgtgcac gtagagtgcg accatcgaca aagaccctcg
     3301 ttggaagaac tcgaacatgg ctcccccgtc cttcgactcc cgggccgcgg ccgaacgctg
     3361 gcaccgcttc accgccgcga cggtgcgcgc gttccaccgc tacggcacct ggctggtgtc
     3421 gctgagctgg tggcgcttcg ggtggtactc gctgctgctg ctggtcgcct cgggcgtgat
     3481 ccagaagctg ccgcccttca gctggacgta cacggagacc gtgaccgaga gcgcccgcca
     3541 ccgccacgcc cgaaccgcgc cgacgccgcc cgctgcgccc tccttcgccg cgccgttcgt
     3601 cccccccgtg ccgcc
//
LOCUS       DASVLN010000979         7423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_482468, whole genome shotgun sequence.
ACCESSION   DASVLN010000979 DASVLN010000000
VERSION     DASVLN010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482468"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VF453_23020"
     CDS             <1..1504
                     /locus_tag="VF453_23020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX7690595.1"
                     /translation="DLPLSALEDGSHPAAQRLKFEELLAQQISQLAARAERALLQAPV
                     IVPRAGGLQDRLLDVLPFALTGAQRRVVAEIVADLAGPRPMHRLLQGDVGAGKTVVAA
                     LAAAAAIDAGWQCALMAPTEILAEQHFAKLVQWLEPLGIGVAWLSGSRKGRARAQALE
                     RTASGDAKLVVGTHAVIQDDVAFARLGLAVVDEQHRFGVQQRLALRRKLAEGGLEPHL
                     LMMTATPIPRTLAMTYFADLDVSTIDELPPGRTPIVTRVFADVRRPDVVDRIRDEIAA
                     GRQVYWVCPLIEESEEAENAAAEDSGREPITLQNVTETHAALAQALPGFEVGLLHGRM
                     KAADKAAVMARFKSGELPVLVATTVIEVGVDVPNASLMVIEHAERFGLAQLHQLRGRV
                     GRGSAASVCVLLYAGQLSHVAKDRLRAMVETTDGFEIARRDLEIRGPGEFMGARQSGD
                     QLLRFADLQEDAPLLAHVRRVAPELLARAPGAASAHVERWLGGKLEWMKA"
     gene            1664..2647
                     /locus_tag="VF453_23025"
     CDS             1664..2647
                     /locus_tag="VF453_23025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7690596.1"
                     /translation="MTLTELRYIVAVARERHFGRAAEACFVSQPTLSVAIKKLEEELD
                     VKIFERGSSEVSLTPLGTAIVRQAQSVIEQAAAIKEIAKRGQDPLTGPLRLGIIYTIG
                     PYLLPQLVRHAIALTPQMPLMLQENFTVKLLDMLRTGELDCAIMAEPFPDSGLAVAPL
                     YDEPFVVAVPRTHRLAQETAISADTLKQETMLLLGTGHCFRDHVLEVCPEFARFSNDA
                     EGIRKSFEGSSLETIKHMVASGMGVTVVPKLSIPHGLHDPDSHALVTYVPFAPPVPTR
                     RVVLAWRRSFPRYEAIAALRNAVYACDLEGVTRLTDLAQAHDKAARAEDAA"
     gene            complement(2703..4688)
                     /locus_tag="VF453_23030"
     CDS             complement(2703..4688)
                     /locus_tag="VF453_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008453181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7690597.1"
                     /translation="MFSDLIHGAWTPLSVTLAAVGVGLGIVLVLWLLATLTGIVRYIP
                     NDRIGVVEKLWSGSGSVRSGLLALSGEAGFQPELLRGGFHFFAPLQYRVHICPMVSVT
                     QGKIGYVFARDGVDLPAGQTLADNARIDHFLDVRAFLQGGGQKGPQRKVLREGVHIIN
                     PALFVVMTEEATYSMTMDTTEREYYQKMRDLLDQRRGFSPVVIKGADDERASDLLAVV
                     TVQDGPALPRNELLAPDVGDNHNSFQEPEKFLAAGGRRGRQERVLVEGTYYINRLFAT
                     VEMITKTVIPVGYVGVVVSYTGRKGADVSGDKYSHGELVETGCRGVWRDPLMPGKYAF
                     NTYAGKIELVPTTNFVLMWQTGMTGSNFDSNLREITLITKDAFEPLLPLSVVVHIDYR
                     KAPMVVQRFGNVKQLVEQTLDPMVSAYFKNVSQRRTFIELIQSRSELQAAASIDMRER
                     FQAYSLEFQEVLIGTPKPQQGDRKIEDIMSQLRDRQIAREQVETFAQKQIAADKQREL
                     NESETRAAKQADLTASLVDISIKDNHGAALVKAAERRRQEVESLAQAEKFRQENEGAG
                     RASAIKSVGEAEAAAIKAKSEALEGEGADKQLMQTVMLRLAEAFETARVPLVPQIQVG
                     GATGGDGGNAFATLLSLMNSLKAAELARLGDETKRAA"
     gene            5290..6519
                     /locus_tag="VF453_23035"
     CDS             5290..6519
                     /locus_tag="VF453_23035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7690598.1"
                     /translation="MDRLLRWLPADVDPAAGWLLASRSLRGFADGFVAVLLPAYLSAL
                     GLGLVQIGVLSTLTLAGSAAATIALGALGHHVASTRLMRIAAATMVATGLGFAGFAAF
                     WPLAAIAFVGTLNPSSGDVSVFLPLEHARLAHAARTSVARTALFARYALLGNLSAALG
                     SLASALPSWWHARLHVDLLAGMRAMFVLYAATGAIVALLYRRLESQRRADDAATEAPP
                     QPLGPSRGIVVRLAALFCVDSFAGGLVVNSMLALWLYRRFGLAVGDAGAFFFVAGILV
                     TASQLVAPALARRVGLVRTMVYTHIPANGFLMLAALAPTLPLAIALLLARSALQAMDV
                     PARSAFVMAVVTPPERPAAASFTAVPRSLAAAAAPTITAALLASGVAAAPLLVGGALK
                     IGYDLALLAMFRRHPVD"
     gene            complement(6526..>7423)
                     /locus_tag="VF453_23040"
     CDS             complement(6526..>7423)
                     /locus_tag="VF453_23040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7690599.1"
                     /translation="DAARAEVAHDEPGHDRRKHRWLSLDTPFLAALGVTDARAALVPA
                     MARKWKQINKFVEVMAHALESAGFDARSPLNVVDFGAGKGYLTFALHDWLRRQDGREP
                     HSLDIVGVELRPELVRLCNEVVAQCGLQGLRFEAGDVSSFVPARLDVMIALHACDTAT
                     DHAIHTGIRAGAAIVMCSPCCHKQVRPQMHLPPLLKPMLQHGIHLGQQAEMVTDALRA
                     LLLEAHGYDAQVFEFVALEHTSKNKMILAVKRRERADSPQALRRRGQALAQVAELKRF
                     YGIEEQCLERLLAEKESAAAAG"
BASE COUNT          968 a         2679 c         2661 g         1115 t
ORIGIN      
        1 cgacctgccg ctgtcggcgc tggaggacgg cagccacccg gcggcgcagc gcctgaagtt
       61 cgaggagctg ctggcgcagc agatctcgca gctcgccgcc cgtgccgagc gcgcgctgct
      121 gcaggcgccg gtcatcgttc cgcgcgcggg cggcctgcag gacaggctcc tcgacgtgct
      181 gccgttcgcg ctcaccggcg cacagcggcg cgtggtcgcg gagatcgtcg ccgacctcgc
      241 cggcccgcgg ccgatgcacc gcctgctgca gggtgacgtc ggcgccggca agacggtggt
      301 cgccgcgctc gccgcggcgg cggccatcga cgccggctgg cagtgcgcgc tgatggcgcc
      361 aaccgagatc ctcgccgagc agcacttcgc caagctcgtg caatggctcg agccgctggg
      421 catcggggtc gcctggctca gcggcagcag gaagggcagg gcgcgggcgc aggcgctcga
      481 gcgcaccgcc tcgggcgacg cgaagctggt cgtcggcacg catgccgtga tccaggacga
      541 cgtcgccttc gcgcggctgg ggctggccgt cgtcgacgag cagcaccggt tcggcgtgca
      601 gcagcggctc gcgctgcggc gcaagctggc cgagggcggg ctggagccgc acctgctgat
      661 gatgacggcc acgcccatcc cgcgcaccct ggcgatgacc tacttcgccg atctcgacgt
      721 cagcacgatc gacgagctgc cgcccggccg cacgcccatc gtcacgcggg tgttcgcgga
      781 cgtgcgccgg cccgacgtcg tcgatcgcat ccgcgacgag atcgccgccg gccgccaggt
      841 ctactgggtg tgcccgctga tcgaggagtc ggaggaggcc gagaacgcgg cggccgagga
      901 ctccggccgg gagccgatca cgctgcagaa cgtcaccgag acccacgccg cgctcgcgca
      961 ggcgctgccg ggcttcgagg tcggcctgct gcacgggcgc atgaaggccg ccgacaaggc
     1021 cgcggtgatg gcgcgcttca agtcgggcga gctgcccgtg ctggtcgcga ccaccgtgat
     1081 cgaggtcggc gtcgacgtgc ccaacgcgag cttgatggtg atcgagcacg ccgagcgctt
     1141 cggcctcgcc cagctgcacc agctgcgcgg gcgggtcggc cgcggcagcg cggcgagcgt
     1201 gtgcgtgctg ctgtacgccg gccagctgtc gcacgtggcg aaggatcggc tgcgggcgat
     1261 ggtcgagacc accgacggct tcgagatcgc gcgccgcgac ctcgagatcc gcgggcccgg
     1321 cgagttcatg ggcgcccggc agtccggcga ccagctgctg cgcttcgccg acctgcagga
     1381 ggacgccccg ctgctcgccc atgtccgccg cgtcgcgccg gagctgctcg cccgcgcgcc
     1441 cggcgccgcg tccgctcatg tcgaacgctg gctcggcggc aagctcgaat ggatgaaggc
     1501 ctagccacaa gggcgcatac ttggccggcg ctgccgctca tccggggtca acgggatccg
     1561 ggggagcggc ggatccggct ccgccgggcc gtgcgcggcc ggcccctcgg cgggcaggga
     1621 gcgctagcga ccgtggggcc gtccattaca caatagcgtc atcatgactc tcaccgagct
     1681 gcgctacatc gtcgccgtcg cccgcgagcg gcacttcggc cgcgcggccg aggcttgctt
     1741 cgtctcgcag ccgacgctgt cggtggcgat caagaagctg gaggaggagc tcgacgtcaa
     1801 gatcttcgag cgcggcagca gcgaggtgag cctcacgccg ctcgggacgg ccatcgtgcg
     1861 ccaggcgcag agcgtgatcg agcaggccgc cgcgatcaag gagatcgcca agcgcggcca
     1921 ggatccgctg accggcccgc tgcggctggg catcatctac acgatcgggc cctacctgct
     1981 gccgcagctg gtgcgccacg cgatcgcgct gacgccgcag atgccgctga tgctgcagga
     2041 gaacttcacc gtcaagctgc tggacatgct gcgcaccggc gagctcgatt gcgccatcat
     2101 ggccgagccg ttccccgaca gcggcctggc cgtcgcgccg ctgtacgacg agcccttcgt
     2161 cgtcgccgtg ccgcgcacgc accgcctggc gcaggaaacc gcgatctcgg ccgacaccct
     2221 gaagcaggag acgatgctgc tgctgggcac cggccactgc ttccgcgacc acgtgctcga
     2281 ggtctgcccg gagttcgcgc gcttctccaa cgacgccgag ggcatccgca agagcttcga
     2341 gggttcgtcg ctggagacga tcaagcacat ggtcgcctcg ggcatgggcg tgacggtggt
     2401 gcccaagctg tcgatcccgc acggcctgca cgacccggat tcgcacgcgc tggtcaccta
     2461 cgtgcccttc gcgccgccgg tgcccacgcg ccgcgtggtg cttgcctggc gccgcagctt
     2521 cccgcgctac gaggcgatcg cggcgctgcg caacgcggtg tacgcctgcg acctcgaagg
     2581 cgtgacgcgg ttgacggacc tggcgcaggc gcacgacaaa gccgcccgcg ccgaagacgc
     2641 ggcctgatcc cgaggccagg cgacgccagg atgcgcggca ggcggatgcc cgccgcgcgc
     2701 gttcaggccg cgcgcttcgt ttcgtcgccc aggcgcgcga gctccgcggc cttgagcgag
     2761 ttcatcagcg acagcagcgt ggcgaacgcg ttgccgccgt cgccgcccgt cgcgccaccg
     2821 acctggatct gcggcaccag gggcacgcgc gcggtctcga aggcctcggc caggcgcagc
     2881 atcacggtct gcatcagctg cttgtcggcg ccttcgcctt ccagcgcctc ggacttggcc
     2941 ttgatcgccg cggcttccgc ctcgccgacc gacttgatcg ccgacgcgcg gcccgcgccc
     3001 tcgttctcct ggcggaactt ctcggcctgc gcgagcgact cgacctcctg gcgccggcgt
     3061 tccgcggcct tcacgagcgc cgcgccgtgg ttgtccttga tggagatgtc caccagcgac
     3121 gcggtcaggt cggcctgctt ggcggcgcgc gtctcgctct cgttgagctc gcgctgcttg
     3181 tcggccgcga tctgcttctg cgcgaaggtc tcgacctgct cgcgcgcgat ctggcggtca
     3241 cgcagctggg acatgatgtc ctcgatcttg cggtcgccct gctgcggctt cggggtgccg
     3301 atcagcactt cctggaactc gagcgagtag gcctggaagc gctcgcgcat gtcgatcgac
     3361 gcggcggcct gcagctcgct gcgcgactgg atcagctcga tgaaggtacg gcgctgcgac
     3421 acgttcttga agtacgccga gaccatcggg tcgagcgtct gctcgacgag ctgcttcacg
     3481 ttgccgaagc gctgcacgac catcggcgcc ttgcggtagt cgatgtgcac gaccaccgac
     3541 aggggcagca gcggctcgaa ggcgtccttg gtgatcagcg tgatctcgcg caggttcgag
     3601 tcgaagttcg agcccgtcat gccggtctgc cacatgagca cgaagttggt cgtggggacg
     3661 agctcgatct tgccggcgta ggtgttgaac gcgtacttgc cgggcatcag cgggtcgcgc
     3721 cacaccccgc ggcagccggt ctccaccagc tcgccgtgcg agtacttgtc gcccgagacg
     3781 tcggcgccct tgcggcccgt gtacgacacg acgacgccga cgtagccgac cgggatcacg
     3841 gtcttggtga tcatctcgac ggtcgcgaac aggcggttga tgtagtatgt gccttcgacg
     3901 agcacccgct cctggcgtcc gcggcggccg ccggcggcga ggaacttctc cggttcctgg
     3961 aacgagttgt ggttgtcgcc cacgtccggc gcgagcagct cgttgcgcgg cagggcgggg
     4021 ccgtcctgca ccgtcacgac ggccagcagg tcggaggcgc gctcgtcgtc cgcgcccttg
     4081 atgacgaccg gcgagaagcc gcggcgctgg tccagcaggt cgcgcatctt ctggtagtac
     4141 tcgcgctcgg tggtgtccat cgtcatcgag taggtcgcct cctcggtcat cacgacgaac
     4201 agcgcggggt tgatgatgtg cacgccctcg cgcagcacct tgcgctgcgg gcccttctgg
     4261 ccgccgccct gcaggaaggc gcggacgtcg aggaagtggt cgatgcgggc gttgtccgcg
     4321 agcgtctggc cggcaggcag gtcgacgccg tcgcgcgcga agacgtagcc gatcttgccc
     4381 tgcgtcaccg acaccatcgg gcagatgtgc acgcggtact gcagcggggc gaagaagtgg
     4441 aagccgccgc gcagcagctc gggctggaag ccggcctcgc cgctcagcgc gagcagcccc
     4501 gagcgcaccg agccgctgcc gctccacagc ttctcgacga cgccgatgcg gtcgttgggg
     4561 atgtagcgga cgatgcccgt gagcgtggcg agcagccaca gcacgagcac gatgccgagc
     4621 ccgacgccca cggcggcgag cgtcacggaa agcggcgtcc aggcgccgtg gatcagatcg
     4681 gaaaacatga gttccttcct cgggcgctgg gcccgtcgtt gtcatgcgcc ccgggcgcgg
     4741 ctggcgccgc gacggccttg cgcatcctct tgcgcgcggc ggccgtcgac acgggtgcgc
     4801 gaaggcgcgg cctctggcag ccggcgcatt gcgcttcgga ccgcgccggc gagcgcgtgc
     4861 ggatccccgt cgcgcctcgc acgagcgaca cgcggacgag cctgcgcaca cgcgcgcggg
     4921 gcgcgtgccg agacgttcag tggagggacg gcgggtgcgc cgtcaggcct gccccggtgg
     4981 gactcgccgg tgtcggcagg ccacgggctt gcaggcgccg tgcgccgcgg gcgccacgcc
     5041 cgggcggtga agggtcatgg agcaagcggc gtcgcaggcc accaggccgg aatcgccgat
     5101 cgatgcgacg ccgagggcgc cacggatgcg aaagttacgc aagtctcact cactgtctgc
     5161 gaccgccaag ggcccgacgg ttgagcatct tgcgcttcgt gttgcgctgc gtcaatgggg
     5221 agccgcggcg ctttggagag cgctcccgaa acgcgcgcaa gcgcccgctg gcaccgacaa
     5281 tcgcgcgcca tggaccgctt gctgcgatgg ctgcccgccg acgtcgatcc cgccgcgggc
     5341 tggctgctcg cgagccgctc gctgcgcggc ttcgccgacg gcttcgtcgc cgtcctgctg
     5401 ccggcctacc tgtcggcgct cgggctgggg ctcgtgcaga tcggcgtgct gagcacgctc
     5461 acgctggcgg gctccgccgc cgcgacgatc gcgctcggcg cgctggggca ccacgtcgcg
     5521 tcgacgcggc tgatgcgcat cgccgcggcc acgatggtcg ccaccggcct cggcttcgcc
     5581 gggttcgcgg cgttctggcc gctcgccgcc atcgccttcg tcggcaccct caacccgagc
     5641 tcgggcgacg tcagcgtctt cctgccgctg gagcacgcgc gccttgcgca cgccgcgcgc
     5701 accagcgtcg cccgcaccgc gctgttcgcg cgctacgccc tgctgggcaa cctcagcgcc
     5761 gcgctcggct cgctcgcgtc cgcgctgccg tcgtggtggc atgcacgcct gcacgtcgac
     5821 ctgctcgccg gcatgcgcgc gatgttcgtg ctgtacgccg cgaccggggc gatcgtcgcg
     5881 ctgctgtacc gccggctcga atcgcagcgc cgtgccgacg atgccgcgac cgaggcgccg
     5941 ccgcagccgc tcggaccctc gcgcgggatc gtcgtgcgcc tcgcggcgct gttctgcgtc
     6001 gactcgttcg cgggcgggct ggtcgtcaac tcgatgctcg cgctgtggct gtaccgacgc
     6061 ttcgggctcg cggtgggcga cgcgggcgcg ttcttcttcg tcgccggcat cctcgtgacc
     6121 gcctcgcagc tcgtcgcgcc ggcgctcgcg cggcgcgtcg gactcgtgcg caccatggtc
     6181 tacacgcaca tcccggccaa tgggttcctg atgctggccg cgctggcgcc gacgctgccg
     6241 ctcgcgatcg cgctgctgct cgcccgcagc gcgctgcagg cgatggatgt gccggcccgc
     6301 agcgccttcg tgatggccgt ggtgacgccc cccgagcgcc cggccgccgc cagcttcacc
     6361 gccgtcccgc gcagcctggc cgcggcggcg gcgccgacga tcacggcggc cctgctcgcg
     6421 agcggcgtcg ccgcggcgcc cctgctggtc ggcggcgcgc tgaagatcgg ctacgacctg
     6481 gcgctgctgg cgatgttccg ccgccacccg gtggactgat ccggcctaac cggccgctgc
     6541 agcggattcc ttctccgcga gcaagcgctc caggcactgc tcctcgatgc cgtagaagcg
     6601 cttgagctcc gcgacctgcg cgagcgcctg cccgcgccgg cgcagggcct gcggcgaatc
     6661 ggcgcgctcg cggcgcttca cggcgaggat catcttgttc ttgctggtgt gctcgagcgc
     6721 gacgaactcg aacacctgcg cgtcgtagcc atgcgcctcc agcagcagcg cgcgcagcgc
     6781 gtcggtgacc atctcggcct gctggcccag gtggatgccg tgctgcagca tcggcttgag
     6841 cagcggcggc aggtgcatct gagggcgcac ctgcttgtgg cagcagggcg agcacatgac
     6901 gatcgcggcg cccgcgcgga tgccggtgtg gatcgcgtgg tcggtcgcgg tgtcgcaggc
     6961 gtgcagggcg atcatcacgt ccaggcgcgc gggcacgaag ctcgagacgt cgcccgcctc
     7021 gaagcgcagg ccctgcaggc cgcattgcgc gaccacctcg ttgcacaggc gcacgagctc
     7081 gggccgcagt tcgacgccga cgatgtccag cgaatgcggc tcgcggccgt cctggcgccg
     7141 cagccagtcg tgcagcgcga acgtcaggta gcccttgccg gcgccgaagt cgacgacgtt
     7201 cagcggcgag cgcgcgtcga acccggccga ctccagcgca tgcgccatca cctcgacgaa
     7261 cttgttgatc tgcttccact tgcgcgccat ggccggcacc agcgcggcgc gcgcatcggt
     7321 gacgccgagc gcggcgagga agggcgtgtc caggctcagc cagcggtgct tgcggcggtc
     7381 gtgcccgggc tcgtcgtgcg cgacctccgc gcgcgccgcg tcg
//
LOCUS       DASVLN010000980         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_212942, whole genome shotgun sequence.
ACCESSION   DASVLN010000980 DASVLN010000000
VERSION     DASVLN010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212942"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2491)
                     /locus_tag="VF453_23045"
     CDS             complement(<1..2491)
                     /locus_tag="VF453_23045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7690600.1"
                     /translation="MTARLTTSEAEDAGGTLAFLAGGGRMGELIAAFDWSRTAMGRIA
                     DWPAHVRSATSLMLRSRVPIVMLWGEPGVMVYNDAYSVFAGKRHPALLGSNVREGWPE
                     VADFNDHVMKVGLAGGNLEYRDQELTLHRHGRPEPAWMNLDYSPLLDERGVPAGVMAV
                     VVETTEKVLAERAVSGERARLAQLFALAPSFMAVVEGPEHRFALANASYLKLVGLPAI
                     VGRTVAEALPGAAEQGWVALLDQVYRSGEAFSGYGARYDRPSAPGAPPEARYVDFILQ
                     PIRGQGGEVTGIFMEGVDATSRVAAEQRRDALVRLSERLRDLEDPAAIGRLAAELIGA
                     TLHVARAGYAAVDPGGDTLRVERDWTAPGIASTVGVHPLHSPGVFVDALRRGEFVVVV
                     DVREEPRTAAVAAVLESRHARAYVHVPVLEQGRLVALLYVDHPAPRAWTADDIDFARE
                     VAQRTRMAFERARTERARRAAARAREDALAEVAAIDQKFRALVTASSDAVYRMSADWS
                     QMRELDGRDFLPHTDEPSADWLELYIPADERPRVRGAIAHAIATRTPFELEHQVVHAD
                     GTIGWTFSRAIPVVDDHGEVVEWFGAASDVTRRKRAERELHESEERLRETDRRKDEFL
                     ATLAHELRNPLAPIRNAVHLLALPGGAAHLPRLREMLERQVGHMVRLVDDLLEISRVS
                     RGQITLRQEDVDLGAVIDAAVEASRPLVDQAHHHLVVVPAPQALWVRGDAMRLSQVFV
                     NLLNNAARYTDPGGVIELACAQQAGWLAVTVTDNGVGIAADQLPRLFEMFTQIDRTHS
                     RAQGGLGIGLALSRRLLEMHGGTLEAASAGLG"
     gene            complement(2539..>2914)
                     /locus_tag="VF453_23050"
     CDS             complement(2539..>2914)
                     /locus_tag="VF453_23050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7690601.1"
                     /translation="VAGRVAPRRVLVVDDNADAADSMGDMLRLMGHEVLVAHAAGAAL
                     AQVDAFAPDWAMLDLNMPDMDGCTLLHALRERPGLADLRAAAVTGYGQPQDRERSLVA
                     GFDAHLIKPVEIAQVGALMASP"
BASE COUNT          374 a         1079 c         1084 g          377 t
ORIGIN      
        1 ggccgaggcc ggcgctcgcg gcctcgagcg tgccgccgtg catctccagc aggcgccgcg
       61 acagcgccag cccgatgccg agcccgccct gggcgcggct gtgggtgcgg tcgatctgcg
      121 tgaacatctc gaacagccgc ggcagctggt cggcggcgat gccgaccccg ttgtcggtga
      181 cggtgaccgc cagccagccc gcctgctgcg cgcaggcgag ctcgatcacc ccgccggggt
      241 cggtgtagcg ggccgcgttg ttcagcaggt tcacgaacac ctggctgagg cgcatcgcgt
      301 cgccgcgcac ccacagcgcc tgcggcgccg gcacgacgac gaggtggtgg tgggcctggt
      361 cgacgagcgg ccggctcgcc tcgaccgccg cgtcgatgac cgcgccgagg tcgacgtcct
      421 cctggcgcag cgtgatctgg ccgcggctga cgcgcgagat ctcgagcagg tcgtcgacga
      481 ggcgcaccat gtggccgacc tggcgctcga gcatctcgcg cagccgcggc aggtgcgccg
      541 ccccgcccgg cagggccagc aggtggacgg cgttgcggat cggcgcgagc gggttgcgca
      601 gctcgtgggc gagcgtcgcg aggaattcgt ccttgcggcg gtcggtctcg cgcaggcgct
      661 cctcgctctc gtggagctcg cgctcggcgc gcttcctgcg cgtgacgtcg ctcgccgcgc
      721 cgaaccattc gacgacctcg ccatggtcgt cgaccaccgg gatcgcgcgc gagaacgtcc
      781 agccgatcgt gccgtccgcg tggacgacct ggtgctcgag ctcgaacggc gtgcgcgtcg
      841 cgatcgcgtg cgcgatggcg ccgcgcacgc gcggtcgctc gtcggccggg atgtagagct
      901 cgagccagtc ggcggacggc tcgtcggtat ggggcaggaa gtcgcggccg tcgagctcgc
      961 gcatctgcga ccagtcggcg ctcatgcggt agacggcgtc ggagctcgcc gtgacgagcg
     1021 cgcggaactt ctggtcgatc gccgcgacct cggcgagcgc gtcctcgcgt gcccgggctg
     1081 cggcgcggcg cgcgcgctcg gtgcgggcgc gctcgaaggc catgcgcgtg cgctgggcca
     1141 cctcgcgagc gaagtcgatg tcgtcggccg tccaggcgcg cggtgcgggg tggtcgacgt
     1201 acagcagcgc gacgagccgt ccctgctcca gcaccggcac gtgcacgtag gcgcgcgcgt
     1261 gccgcgactc cagcacggcg gcgaccgcgg cggtgcgcgg ctcctcgcgc acgtcgacga
     1321 cgaccacgaa ctcgccgcgc cgcagcgcgt cgacgaacac gccgggcgaa tgcagcggat
     1381 ggacgccgac cgtggaggcg atgcccggcg ccgtccagtc gcgctccacg cgcaacgtgt
     1441 cgccgccggg atcgacggcg gcgtagccgg cgcgcgcgac atgcagcgtc gcgccgatca
     1501 gctccgccgc caggcggccg atggcggcgg ggtcctcgag gtcgcgcagc cgctccgaca
     1561 gccgcacgag cgcatcgcgg cgctgctccg ccgcgacgcg gctcgtcgcg tccacgccct
     1621 ccatgaagat gcccgtcacc tcgccgccct ggccgcggat cggctggagg atgaagtcga
     1681 cgtagcgcgc ctccggcggc gcgcccggcg cgctggggcg gtcatagcgc gcgccgtagc
     1741 cggagaacgc ctcgccggag cggtagacct ggtccagcag cgcgacccag ccctgctccg
     1801 ccgcgccggg cagcgcctcg gcgacggtgc ggccgacgat ggcgggcagg cccaccagct
     1861 tcaggtagct cgcgttggcg agcgcgaagc ggtgctccgg gccctcgacg accgccatga
     1921 agctgggcgc cagcgcgaac agctgcgcga ggcgcgcgcg ctcgccgctg accgcgcgct
     1981 cggccagcac cttctcggtc gtctcgacca ccaccgccat cacgccggcg ggaacgccgc
     2041 gctcgtcgag cagcggcgag tagtcgaggt tcatccacgc cggctccgga cggccgtggc
     2101 ggtgcagcgt gagctcctgg tcgcggtact ccaggttgcc gccggccagg ccgaccttca
     2161 tcacgtggtc gttgaagtcg gcgacctcgg gccagccctc gcgcacgttc gagcccagca
     2221 gcgccggatg gcgcttgccg gcgaacaccg agtaggcgtc gttgtagacc atcacgcccg
     2281 gctcgcccca cagcatcacg atgggcacgc gcgagcgcag catcagcgag gtggccgagc
     2341 gcacatgcgc cggccagtcg gcgatgcggc ccatcgcggt tcggctccag tcgaaggcgg
     2401 cgatcagctc gcccatgcgg ccgccgccgg cgaggaacgc gagcgtcccg ccggcatcct
     2461 ccgcctccga cgtcgtgagc cgtgccgtca tctggagata ccgtctgccg tatcaggaaa
     2521 ttctggcatg gccgggcatc acggcgatgc catcagcgcg ccgacctggg cgatctccac
     2581 cggcttgatc aggtgggcgt cgaagccggc gacgagcgag cgctcgcggt cctgcggctg
     2641 gccgtagccg gtgacggcag cggcgcgcag gtccgcgagc cccggccgct cgcgcagcgc
     2701 gtgcagcagc gtgcagccgt ccatgtcggg catgttgagg tcgagcatcg cccagtccgg
     2761 cgcgaacgcg tcgacctgcg ccagcgccgc gccggccgcg tgcgcgacca gcacctcgtg
     2821 gcccatcagg cgcagcatgt cgcccatgct gtcggcggcg tcggcgttgt cgtcgacgac
     2881 cagcacccgc cgcggcgcca cgcggccggc gacg
//
LOCUS       DASVLN010000981         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_428450, whole genome shotgun sequence.
ACCESSION   DASVLN010000981 DASVLN010000000
VERSION     DASVLN010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428450"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VF453_23055"
     CDS             complement(<1..1324)
                     /locus_tag="VF453_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX7690602.1"
                     /translation="MAEAAAPAPAHAEPNAHASDWVAVFAGALGALMATLDISITNSA
                     LPQIQGAVGATGTEGTWISTGYLMSEIVMIPLAAWLTRVFGLRRFLLGNAALFTAFSV
                     LCGLSDGLAVLVLARIGQGFTGGAMIPTAQTIIRTRLPRHQMPVGMTMFGLIVLLGPL
                     LGPVVGGALTEGASWRWCFFINVPVCFTLIALLVLGLPADRARLKDFLEADWLGIAGL
                     ALGLSTLTVVLEEGQRERWFESSMIVALSVLSSVGMLLIAASQLTAARPILRLALLRN
                     RHYASVILIVFTVGAGLYCVSYLVPQFLSLIAGYNAQQSGAVMLLAGVPAILMMPMLP
                     RLLGKVDFRVLVIAGLGCFAASCLLDTTLTAQSVGADFTGSQLLRGVGQMLAMMPLNQ
                     ASMAAVSREESGDAAGLYNMARNLGGSVGLAVIGTFIDRREALHGEMVR"
     gene            complement(1317..>1925)
                     /locus_tag="VF453_23060"
     CDS             complement(1317..>1925)
                     /locus_tag="VF453_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX7690603.1"
                     /translation="NTQAQAEASLREDEAAVKSARGRLASSAAQAAQAEAQVKAGEAS
                     LRQSGLEVDDTVVRAAIDGRIGDSTVRVGQFVQPGTRLMTVVPVQQVYLVANFKETQI
                     ARMRAGQPVTVRVDALPGVDLHGVVESFSPGTGSQFALLPPENATGNFTKIVQRVPVR
                     VRLEAPPEARERLVPGLSVTAEVDTRSGMGDEHRLADEGRRG"
BASE COUNT          298 a          716 c          677 g          234 t
ORIGIN      
        1 cgcgcaccat ctcgccgtgc agggcctcgc ggcgatcgat gaaggtgccg atgacggcga
       61 ggccgaccga gccgccgagg ttgcgcgcca tgttgtacag gccggcggcg tcgcccgatt
      121 cctcgcgcga taccgcggcc atcgaggcct ggttcaacgg catcatcgcg agcatctggc
      181 cgacgccgcg cagcagctgc gagccggtga agtcggcgcc gacgctctgt gcggtgaggg
      241 tggtgtccag caggcagctc gccgcgaagc agcccagccc ggcgatcacg aggacgcgga
      301 agtcgacctt gcccagcagg cgcggcagca tcggcatcat caggatcgcc ggcacgcccg
      361 ccagcagcat caccgcgccc gactgctgcg cgttgtagcc ggcgatcagg ctcaggaact
      421 gcggcacgag gtaggacacg cagtacagcc ccgcgcccac ggtgaacacg atcaggatca
      481 cgctcgcata gtgccggttg cgcagcagcg ccagccgcag gatgggccgc gcggccgtga
      541 gctgcgacgc ggcgatcagc agcatgccga cggacgacag cacgctcagc gccacgatca
      601 tcgacgactc gaaccagcgc tcgcgctgcc cttcctcgag caccacggtc agcgtgctca
      661 ggcccagcgc gaggcccgcg atgccgagcc agtcggcctc gaggaagtcc ttcagccgcg
      721 cgcgatccgc aggcagcccc agcaccagca gcgcgatcag cgtgaagcac accggcacgt
      781 tgatgaagaa gcaccagcgc cagctcgcgc cctcggtcag cgcgccgccc acgaccggcc
      841 cgagcagcgg cccgagcagc acgatcaggc cgaacatcgt catgcccacg ggcatctggt
      901 ggcgcggcag gcgggtgcgg atgatggtct gcgccgtcgg gatcatcgcg ccgccggtga
      961 agccctggcc gatgcgcgcg agcaccagca cggcgagccc gtcggacagc ccgcacagca
     1021 ccgagaacgc ggtgaacagc gccgcgttcc cgagcaggaa cctgcgcaga ccgaacacgc
     1081 gggtgagcca cgccgcgagc gggatcatga cgatctcgga catcaggtag ccggtcgaga
     1141 tccaggtgcc ctcggtgccc gtcgcgccga ccgcgccctg gatctgcggc agcgccgagt
     1201 tggtgatcga gatgtccagc gtcgccatca gcgcgccgag cgcgccggcg aagacggcca
     1261 cccagtcgga cgcatgcgcg ttcggctccg cgtgcgcggg cgcgggcgcg gcggcctcag
     1321 ccacggcggc cctcgtcggc cagccggtgc tcgtcgccca tgcccgaccg ggtatcgacc
     1381 tcggcggtga cggacagccc gggcaccagc cgctcgcgcg cctcgggcgg ggcctcgagc
     1441 cgcacgcgca ccggcacgcg ctgcacgatc ttcgtgaagt tgccggtcgc gttctcgggc
     1501 ggcagcagcg cgaactgcga gccggtgccg ggcgagaagc tctccaccac gccgtgcagg
     1561 tcgacgccgg gcagcgcgtc cacgcgcacc gtcaccggct gcccggcgcg catgcgggcg
     1621 atctgcgtct ccttgaagtt ggcgacgaga tagacctgct gcaccggcac caccgtcatc
     1681 agccgcgtgc cgggctgcac gaactggccg acgcgcacgg tgctgtcgcc gatgcgaccg
     1741 tcgatggcgg cgcgcacgac cgtgtcgtcg acctcgaggc cgctctggcg caggctcgcc
     1801 tcgccggcct tgacctgggc ctcggcctgc gcggcctgcg cggcgctcga cgcgagccgg
     1861 ccgcgcgcgg acttcaccgc cgcctcgtcc tcgcgcaggc tggcctcggc ctgggcctgc
     1921 gtgtt
//
LOCUS       DASVLN010000982         3008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_374624, whole genome shotgun sequence.
ACCESSION   DASVLN010000982 DASVLN010000000
VERSION     DASVLN010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374624"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..271)
                     /locus_tag="VF453_23065"
     CDS             complement(29..271)
                     /locus_tag="VF453_23065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7690604.1"
                     /translation="MTIVVNLDVMLARRKMRSRELAERVGITEQNISLLKSGKVRGVR
                     FDTLAAICEVLQCQPGDLLEYRPGAPVAAPGDEDAE"
     gene            complement(271..834)
                     /locus_tag="VF453_23070"
     CDS             complement(271..834)
                     /locus_tag="VF453_23070"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX7690605.1"
                     /translation="MTTPAPLPPGAVTAHRIRRLSLLVRGLVAVGALVLAVAVAALWI
                     DPAYALAQIRRDSHLDDIAQLDAVSRLRCVAWMCVTAAVAMMMLLRLWQLFGEYAQGR
                     IFGLRALDRLRGFARWTMAAAVLSPIDGAVVSVLATWNRAIGHRELNVYVSSEEYALL
                     LFGAALLAISSVMTEAARLAEDNAGFV"
     gene            974..1459
                     /gene="ybaK"
                     /locus_tag="VF453_23075"
     CDS             974..1459
                     /gene="ybaK"
                     /locus_tag="VF453_23075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEX7690606.1"
                     /translation="MARKQHVSETPATQWLREHGVTFQEHVYDYVEHGGTAESSAQLG
                     VPEHEVVKTLVMQDEAARPLVVLMHGDRQVSTKNLARAIGCKSVEPCKPEVAQRHSGY
                     QVGGTSPFGFRKDVPVYVQETVLGLPRILINGGRRGFLVEISPDVLVGALGAKPVQCA
                     L"
     gene            complement(1551..>3008)
                     /locus_tag="VF453_23080"
     CDS             complement(1551..>3008)
                     /locus_tag="VF453_23080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX7690607.1"
                     /translation="RLRAKSPLQAHAAAGAADDVHPAQRRYLTRDEYESAHGADDQDI
                     AKVAAFAKEYGLAVVQADAGRRSVVLAGDAAGMAKAFEVSLQEYEHADGTYRGRTGAI
                     SVPAELAGIVEGVFGLDDRPHAQPHFQRHVVSQSLKSVAAKAFTPLELAKLYDFPAGA
                     DGSGQCIGIIELGGGYKPADLKTYFKGLGLAEPKVRAVLVDHAKNHPTNANSADGEVL
                     LDIEVAGAVAPKATIAVYFAPNTDQGFLDAVTTAIHDKLNKPSVISISWGQAESGWTA
                     QSMDQMDQAFQAAAAMGVTICAAAGDTGSGDGVDDGKPHVDFPASSPNVLACGGTKLL
                     AASATAIGSEVVWNEGSTTSATGGGFSTHFAVPAYQSKVASGKSGRGVPDVAGDADPN
                     TGYDVRVDGQDFVIGGTSAVAPLWAGLVAVMNQKLGHPVGFLNPMLYGSLAGKGVTRD
                     ITSGNNGAYKAAAGWDACTGWGSPDGAKLLQALGT"
BASE COUNT          433 a         1063 c         1091 g          421 t
ORIGIN      
        1 catcccggcg cgagcgcggc ggcgggggtc actcggcgtc ctcgtcgccc ggcgcggcga
       61 cgggcgcccc ggggcgatac tcgagcaggt cgcccggctg gcactgcagc acctcgcaga
      121 tcgcggccag cgtgtcgaag cgcacgccgc gcaccttgcc cgacttcagc aaggagatgt
      181 tctgctccgt gatgcccacg cgctcggcga gttcgcgcga gcgcatcttg cggcgcgcca
      241 gcatcacgtc caggttgacg acgatggtca tcagacgaag cccgcgttgt cctcggccag
      301 gcgcgcggcc tctgtcatca cgctcgagat cgcgagcagc gcggcgccga acagcagcag
      361 cgcgtactcc tcggacgaga cgtagacgtt cagctcccgg tgcccgatcg cgcggttcca
      421 ggtcgcgagg acggagacca ccgcgccgtc gatcggcgag agcaccgccg ccgccatcgt
      481 ccagcgcgcg aagccgcgca gccggtcgag cgcgcgcagg ccgaagatgc ggccctgcgc
      541 gtactccccg aacagctgcc acagccgcag cagcatcatc atcgcgaccg ccgcggtcac
      601 gcacatccac gcgacgcagc gcaggcgcga gaccgcatcg agctgcgcga tgtcgtccag
      661 gtgcgagtcg cggcggatct gcgccagcgc gtaggccggg tcgatccaca gcgccgcgac
      721 cgcgaccgcc agcaccagcg cgccgacggc cacgagcccg cgcacgagca gcgacagccg
      781 gcggatgcga tgcgccgtga cggcaccggg cggcagggga gcgggagtcg tcatcggtgt
      841 ggactaaaag cgttgcggca ataaattatt gtaaaacgat aaaatatgat tgcgcaacgg
      901 gatcaagcgc gcgatgacaa cggacaagag ccacgcgaca tgcccggccc ggcatgcgcg
      961 cgacaatcgc ccgatggccc gcaagcaaca cgtcagcgaa acccccgcca cccagtggct
     1021 gcgcgagcac ggggtgacct tccaggagca cgtctacgac tacgtcgagc acggcggcac
     1081 ggcggagtct tccgcgcagc tcggcgtgcc cgagcacgag gtcgtcaaga cgctggtgat
     1141 gcaggacgag gcggcgcgtc cgctggtggt gctgatgcac ggcgaccgcc aggtctcgac
     1201 gaagaacctc gcgcgcgcga tcgggtgcaa gtcggtcgag ccgtgcaagc cggaggtggc
     1261 gcagcgccac agcggctacc aggtcggcgg cacgtcgccg ttcggcttcc gcaaggacgt
     1321 gccggtgtac gtgcaggaga ccgtcctcgg actgccgcgc atcctcatca acggcggccg
     1381 gcgcggcttc ctggtggaga tctcgcccga cgtcctggtc ggcgcgctcg gcgcgaagcc
     1441 ggtgcagtgc gcgctctgat cgcgaccggc ccatgcctgg atggaggacg ccccggccgc
     1501 gcgcggtccg gggcgtcggt cgtggcgcgc gcatcacgcg cgcgcggcgc tcaggtgccc
     1561 agggcctgca gcagcttggc gccgtccggc gagccccagc cggtgcacgc atcccagccc
     1621 gccgcggcct tgtaggcgcc gttgttgccg ctggtgatgt cgcgcgtcac gcccttgccg
     1681 gcgagcgacc cgtacagcat cggattgagg aagccgaccg gatggccgag cttctggttc
     1741 atcacggcga ccagcccggc ccacagcggc gcgacggcgc tggtgccgcc gatcacgaag
     1801 tcctggccgt ccacgcgcac gtcgtagccg gtgttcgggt ccgcgtcgcc cgcgacgtcg
     1861 ggcacgccgc gtccgctctt gcccgaggcg accttggact ggtacgccgg caccgcgaag
     1921 tgggtgctga agccgccgcc ggtggcgctg gtggtcgagc cctcgttcca caccacctcg
     1981 gagccgatcg ccgtcgcgct ggcggccagc agcttggtgc cgccgcaggc gagcacgttg
     2041 gggctggacg ccgggaagtc gacgtgcggc ttgccgtcgt cgacgccgtc gccggagccg
     2101 gtgtcgcccg cggccgcgca gatcgtcacg cccatcgcgg cggcagcctg gaaggcctgg
     2161 tccatctggt ccatcgattg cgccgtccag cccgactcgg cctggcccca gctgatcgag
     2221 atcaccgagg gcttgttgag cttgtcgtgg atcgccgtcg tgacggcgtc gaggaagccc
     2281 tggtcggtgt tgggcgcgaa gtagacggcg atcgtggcct tcggcgcgac ggcgccggcg
     2341 acctcgatgt ccagcagcac ctcgccgtcg gcgctgttgg cattggtcgg atggttcttg
     2401 gcgtggtcga ccagcaccgc ccggaccttg ggctcggcga gccccaggcc cttgaagtag
     2461 gtcttcaggt cggccggctt gtagccgccg cccagctcga tgatgccgat gcactggccg
     2521 ctgccgtcgg cgccggcggg gaagtcgtag agcttggcga gctccagcgg cgtgaaggcc
     2581 ttggcggcga cggacttcag gctctggctc acgacgtggc gctggaagtg cggctgggcg
     2641 tgcggacggt cgtccagccc gaacacgccc tcgacgatgc cggcgagctc cgcgggcacc
     2701 gagatcgcgc ccgtgcggcc gcggtaggtg ccgtcggcgt gctcgtactc ctggagcgag
     2761 acctcgaagg ccttggccat gccggccgca tcgccggcga gcaccacgct gcgccggccc
     2821 gcgtcggcct gcacgaccgc caggccgtac tccttggcga acgcggcgac cttggcgatg
     2881 tcctggtcgt cggcgccgtg ggcggactcg tactcgtcgc gcgtcaggta gcggcgctgc
     2941 gcgggatgca cgtcgtcggc ggcgccggcc gcggcgtgcg cctgcagcgg gctcttcgcg
     3001 cgcaggcg
//
LOCUS       DASVLN010000983         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_267416, whole genome shotgun sequence.
ACCESSION   DASVLN010000983 DASVLN010000000
VERSION     DASVLN010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267416"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VF453_23085"
     CDS             complement(<1..461)
                     /locus_tag="VF453_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690608.1"
                     /translation="MPRFIEIQHDEWIAAGPDVVRAHYADLGHRQVARVHPRERLRQL
                     PPGPAGPRYERLLRTPWTVQRDIVEREFRPDGTVVDTCVAGTHRGCTVCARFWRRDDE
                     ERRAPGTLVELTVTQRLRPVVGPLVARWIRRRLERELREFAVEEKADIERVR"
     gene            complement(519..1184)
                     /locus_tag="VF453_23090"
     CDS             complement(519..1184)
                     /locus_tag="VF453_23090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX7690609.1"
                     /translation="MTTLLEERPQTLGEEIANAVSHGIGFLLAIASLPLLVVVAARHG
                     TAVNVVAVSVFSATMIVLYLVSALYHAAPEGRVKAFLNRVDHAAIYLFIAGSYTPFAL
                     GVLHGAWGWTLFGAIWAMAAAGVAVKLLNLLKHPLWSTGLYVAMGWFAIVAAGPLIHA
                     MPWAGLAWLLAGGIAYTAGAVVFMFDSRVPYLHFAWHLFVLAGTTCHFFAALWYAAPA
                     HPA"
BASE COUNT          247 a          524 c          569 g          172 t
ORIGIN      
        1 cgcacccgct cgatgtcggc cttttcctcg accgcgaact cccgcagttc gcgctcgagg
       61 cggcggcgga tccagcgcgc gacgagcggg ccgacgaccg ggcgcagccg ctgcgtcacg
      121 gtgagctcga ccagcgtgcc cggggcgcgg cgctcctcgt cgtcgcggcg ccagaagcgc
      181 gcgcagaccg tgcagccgcg gtgcgtgccg gcgacgcagg tgtcgacgac ggtgccatcc
      241 ggccggaatt cgcgctcgac gatgtcgcgc tgcaccgtcc acggcgtgcg cagcaggcgc
      301 tcgtagcgcg ggcccgccgg gccgggcggc agctggcgaa gccgttcccg tggatgcacg
      361 cgcgcgacct ggcgatggcc gaggtcggca tagtgcgcgc ggacgacgtc cggtccggca
      421 gcgatccact cgtcgtgctg gatctcgatg aagcgtggca cctgggtttc ctccgaggtc
      481 gggcgagcac ggccgccccg gtgccttgcc ggcaagcgtc aggccggatg cgccggcgcc
      541 gcgtaccaga gcgcggcgaa gaagtgacag gtcgtgccgg ccagcacgaa caggtgccag
      601 gcgaagtgca gatagggcac gcgcgagtcg aacatgaaga cgaccgcgcc ggccgtgtag
      661 gcgatgccgc cggccagcag ccacgcgagg cccgcccacg gcatcgcatg gatcagcggg
      721 ccggccgcga cgatggcgaa ccagcccatc gcgacgtaca acccggtcga ccacagcgga
      781 tgcttgagca ggttcagcag cttgaccgcg acgcccgctg ccgccatcgc ccagatcgcg
      841 ccgaacagcg tccagcccca ggccccgtgc aggacgccga gcgcgaacgg cgtgtagctg
      901 cccgcgatga agaggtagat cgccgcatgg tcgacgcggt tgaggaaggc cttgacgcgg
      961 ccctcgggcg ccgcgtggta cagcgcggag acgaggtaga gcacgatcat cgtggccgag
     1021 aacacgctga cggcgacgac gttgaccgca gtgccgtggc gcgccgcgac gacgacgagc
     1081 agcggcagcg aggcgatggc cagcaggaag ccgatgccgt ggctgacggc gttggcgatc
     1141 tcttcgccca gcgtctgggg acgttcttcg aggagggtgg tcatgggtcg ggatcgtacc
     1201 gggtttgcca tgacggcccg aaggccgccg agcatgcggc agcaacgacc gtgcccggaa
     1261 ttccaggcat gcagggtgcc gccgcgatgc gccttccccg ctcaacgcgc agcaggcgcg
     1321 ccgcgacgac acggggctcg gctcgaaacg cgcggaaaca aaagagcgcc ccgcgtgcca
     1381 cgacgcggag cgcaaaccca tgcaaggaag acgaggcagg cgggatgcct gcgcgggtgc
     1441 gggaggccgg acggctgccc ggcgcccgtc agccgtgcgc cacgcgcagc gggcgcgccc
     1501 cggccgcgac cg
//
LOCUS       DASVLN010000984         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_428749, whole genome shotgun sequence.
ACCESSION   DASVLN010000984 DASVLN010000000
VERSION     DASVLN010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428749"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VF453_23095"
     CDS             <1..511
                     /locus_tag="VF453_23095"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345774.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7690610.1"
                     /translation="ALLAGHLRGGGRTVLLEPQALHTETTRLPGLDGRKMSKSLDNAI
                     GMREPPAQVEAKIRGMRTDPQRMRRTDPGEPERCPVWQFHLAYSDEPTRRWAADGCRS
                     AGIGCLDCKQPVVDAVLREQAPWRERAEALLANPKHVHWIVEMGTERARTVARQTMKD
                     VREAVGLAY"
     gene            572..1051
                     /locus_tag="VF453_23100"
     CDS             572..1051
                     /locus_tag="VF453_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HEX7690611.1"
                     /translation="MAGIHITDIESAINWWRGRHPSPDGVTACKEVLALAEVYALMVY
                     YRESEADEDTLPRAARQAWLEWYASTPDAPCIAICSTSQGDSTCKGCGRTFEEVQHWP
                     GMSPGAKRAVWRRITLEAKAWRFNRYAERAHRATGEDHPDEHVFEPVPDTAPGIRTP"
     gene            1186..2484
                     /locus_tag="VF453_23105"
     CDS             1186..2484
                     /locus_tag="VF453_23105"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7690612.1"
                     /translation="MSDVNPHYLDHVVKLSETSDIEASEDIVNGNGVKLLAKGAKIDA
                     RARERLLEFRLSKPLENMMRVVGGTDPSSFGPVAEQLLARHGLLRGICDTAQGIAPLE
                     AVRTVELSTNVQSLLSVYAGQSADKLEHAVGVALLCAAFGDGLSRNPADIGALVISGL
                     AHDVGELYIDPAFLRRDTHLGPQQWKHVATHPIVGSHVLGTMPGAGPRVASVVMAHHE
                     RLDGFGYPQGLLGTSLSMAGQILAVAEMLMGLLESGTQHAARAAVAMKLVPGEFHRRF
                     VDRVARGARAAGESAPQEDVDLATLEDRVARMAATVQHVRQLRDGMQAEMARFGFALR
                     ALVEHAFSRWERIAIGFSSTGLDLAADGDLRAALAQMTPADRMEVGIVLGELRWRIRE
                     LERQLQIRSELFNPTDAERVRALIEQAQQPPAAAMAAVLA"
BASE COUNT          383 a          921 c          887 g          315 t
ORIGIN      
        1 cgcgctgctg gccggccacc tgcgcggcgg cggccgcacg gtgctgctcg agccgcaggc
       61 gctgcacacc gagaccacgc gcctgccggg cctcgacggc cgcaagatga gcaagagcct
      121 ggacaacgcg atcggcatgc gcgagccgcc ggcccaggtc gaggccaaga tccgcggcat
      181 gcgcaccgac ccgcagcgca tgcgccgcac cgaccccggc gagccggagc gctgcccggt
      241 ctggcagttc cacctcgcct actcggacga gccgacccgc cgctgggccg ccgacggctg
      301 ccgcagcgcg ggcatcggct gcctcgactg caagcagccg gtcgtcgacg ccgtcctgcg
      361 cgagcaggcg ccctggcgcg agcgggccga ggcgttgctg gccaatccca agcacgtgca
      421 ctggatcgtc gagatgggca ccgaacgcgc ccgcaccgtc gcgcgccaga ccatgaagga
      481 cgtgcgggag gccgtgggcc tggcctactg agcgcacgca ggcgcagaca agcacagaca
      541 agcccagacg agacggacag gttgcgcatc catggccggc atccacatca ccgacatcga
      601 gtccgccatc aactggtggc gcggccgcca tccgtcgccc gacggcgtca ccgcctgcaa
      661 ggaggtgctg gccctggccg aggtctacgc gctgatggtc tactaccgcg agtccgaggc
      721 cgacgaggac acgctgccgc gcgccgcccg gcaggcgtgg ctcgagtggt acgcatcgac
      781 gcccgacgcg ccctgcatcg cgatctgctc gacctcccag ggcgactcga cctgcaaggg
      841 ctgcggccgc accttcgagg aggtgcagca ctggccgggc atgtcgccgg gcgccaagcg
      901 cgccgtctgg cgccgcatca cgctcgaggc caaggcctgg cgcttcaaca ggtacgccga
      961 gcgggcgcac cgggccacgg gcgaggatca tcccgacgag catgtcttcg agccggtgcc
     1021 cgacaccgcc ccgggtatcc gaactccttg acgggaaccg tgatgggttc cacaccccga
     1081 acgtagggaa tcctggacga ctgctgaatc cgggccacgg gcggccgaag acacggcgat
     1141 gcgtgcgggc ggggaggccg gcgcgcgatt tcaaggaatc gagccgtgtc cgacgtcaat
     1201 ccacattacc tcgaccacgt cgtgaagctc tcggagacct cggacatcga ggcgagcgag
     1261 gacatcgtca acggcaacgg cgtgaagctg ctggccaaag gcgcgaagat cgacgcccgc
     1321 gcgcgcgagc gcctgctcga gttccgcctc agcaagccgc tcgagaacat gatgcgcgtc
     1381 gtcggcggca ccgatccgtc gtccttcggc ccggtcgcgg agcagctgct ggcccgccac
     1441 ggcctgctgc gggggatctg cgacaccgcg cagggcatcg ccccgctgga agcggtccgc
     1501 accgtggagc tgtcgaccaa cgtgcagtcg ctgctgagcg tctatgccgg ccagtcggcg
     1561 gacaagctcg agcacgcggt cggcgtggcg ctgctgtgcg cggcgttcgg cgacggcctg
     1621 tcgcgcaatc ccgccgacat cggcgcgctg gtcatctcgg gtctcgccca cgacgtcggc
     1681 gagctctaca tcgatcccgc gttcctgcgc cgcgacacgc acctcgggcc gcagcagtgg
     1741 aagcatgtcg cgacccaccc catcgtcggc tcgcacgtgc tgggcacgat gccgggcgcg
     1801 ggcccgcgcg tcgccagcgt cgtgatggcg caccacgaac gcctcgacgg cttcggctac
     1861 ccgcagggcc tgctgggcac cagcctgtcg atggccgggc agatcctggc ggtggccgag
     1921 atgctgatgg gcctgctcga atcgggcacg cagcacgcgg cgcgcgcggc cgtggcgatg
     1981 aagctggtgc cgggcgagtt ccaccgccgc ttcgtcgacc gcgtggcccg cggagcgcgc
     2041 gcggcgggcg agtcggcgcc gcaggaggac gtcgacctcg cgacgctcga ggatcgcgtg
     2101 gcgcgaatgg ccgccaccgt acagcacgtg cgccagctgc gcgacggcat gcaggcggag
     2161 atggcacggt tcggcttcgc gctgcgcgca ctggtggagc acgcgttctc gcgctgggag
     2221 cgcatcgcga tcggcttttc cagcaccggc ctcgacctcg ccgccgacgg cgacctgcgc
     2281 gccgcgctcg cccaaatgac gcccgccgac cgcatggaag tcggcatcgt cctgggcgag
     2341 ctgcggtggc gcatccgcga gctcgagcgc cagctgcaga tccgcagcga gctgttcaat
     2401 ccgaccgacg ccgaacgcgt gcgcgcgctc atcgagcagg cgcagcagcc gcccgccgcc
     2461 gcgatggcgg ccgtcctcgc ctgaggctcg cgcggcccgc gcgccg
//
LOCUS       DASVLN010000985         4720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_428759, whole genome shotgun sequence.
ACCESSION   DASVLN010000985 DASVLN010000000
VERSION     DASVLN010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428759"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..944)
                     /gene="asd"
                     /locus_tag="VF453_23110"
     CDS             complement(21..944)
                     /gene="asd"
                     /locus_tag="VF453_23110"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348605.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEX7690613.1"
                     /translation="MKNLLRSLLLQEDLNFLLTNRVPRIALTRFMGWFSKIRNPVVCR
                     LSIATWKLFTDLDLSDARKRRFDSLHDCFTRELVPGARPVDPDPGVLTSPCDAIVGAC
                     GSAAGTEVFQAKGFPYRMEELFGPSNDVAPYRDGTYVTLRLTSAMYHRFHAPQDLAVE
                     HVTYHSGDAWNVNPIALKRVERLFCRNERAVLRTRLARGGHPVAIVPVAAILVASLRL
                     KFLDVLLHLRYKGPNEISLAGHPARFAKGEEMGWFQHGSTIIVFAPRGFELHESIVPG
                     ARLRMGQPLMRLPAMAAAPPRATAGEAVLAG"
     gene            complement(941..2623)
                     /locus_tag="VF453_23115"
     CDS             complement(941..2623)
                     /locus_tag="VF453_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7690614.1"
                     /translation="MDTTFALSAVMLGLLAWTLPRAKRRLELSRAKHASLAGHSRMAK
                     RVAALLPGYAYDEARFFGSDDAPAEVVARRRAGFSRLAALYAERFPRSAAMTAQARDA
                     ISDLQFTGSYRVPYQYSPYLRQHLKTGSFLVKSQGVTVEDLDGNVLYDLTGSYGVNLF
                     GVDFYKACIAEGAARVAELGPVLGAYHPCVLENIAELKRISDKDEISFHMSGTEAVMQ
                     AVRLARYHTRKRNLVRFCGAYHGWWEDVQPGPGNPLPPRDVYTLEEMDDAALHVLRTR
                     KDIACVLVNPLQALHPNKAAPGDSSLVDSGRSAHFDRAAYTAWLRKLREVCTARGIVL
                     IFDEIFVGFRLAPGGAQEYFGVQADMVTYGKTLGGGLPVGVVCGRADLMKRFREDRPA
                     DICFARGTFNAHPYVMGAMNAFLERLRTPEVQALYDGVDERWNARTARFNDRLRGAGL
                     PVRVANLASIWTVLYDKPSRYNWMLQFYLRAHGLALSWIGTGRFIFSLNYRDEDFEAV
                     TERFVAAALEMQADGWWREDPAQSNKAIRRGLLREMLRQRFSTIPTSGRRPR"
     gene            complement(2717..3304)
                     /locus_tag="VF453_23120"
     CDS             complement(2717..3304)
                     /locus_tag="VF453_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690615.1"
                     /translation="MNGFFELLRVQRWDDHRYYHHSLVNQSLHFVSAVSFVVAYGMMF
                     FDPASAALLAWCVSMTTRQAGHFFFEPRGYDHVNQATDEYKEEIKVGYNIQRKIVLMT
                     VWALIPAVLWFEPSLFGLIQPAVDFRGFLHDLGIAWLSLGLTGLIFRVLQLWRTQGLI
                     HGLAWATKIITDPFHDIMLYHGAPLKLMRRHADAT"
     gene            3532..>4720
                     /locus_tag="VF453_23125"
     CDS             3532..>4720
                     /locus_tag="VF453_23125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEX7690616.1"
                     /translation="MDLPTAPARTLRIACVTETYPPEVNGVAVTIARLVEGLHARGHD
                     LQLVRPRQGSDAGAPAPRERFDEVLVRGVPIPRYAGLRMGLPCRRKLVKLWKARRPDL
                     VHIATEGPLGRSALQAARALGLPTCSDFRTNFHAYSTHYGIGWLERPIMGYLRRFHNA
                     TGCTMVPTAALHRELEAEGFRNVTVVARGVDTRRFDPARRDESLRAQWGAGPDQLVVG
                     YIGRLAPEKNLAVLLRAFDAVRAADPRAKLVFVGDGPMRAELLARCPDAHFAGQRTGD
                     DLAAHYASADLFLFPSVTETFGNVTTEAMSSGLAVVAFDYAAAARFIRTEVDGVLVPF
                     GDADAFVAAAARTASDLARCRTMGAQARMTARALDWEGVVAQFEGVIASVLREAATAS
                     APAG"
BASE COUNT          695 a         1607 c         1746 g          672 t
ORIGIN      
        1 cggccgcgcg ccctgcccgc tcaaccggcg agcacggcct cgccggcggt cgctcgcggc
       61 ggcgcggcgg ccatggccgg caggcgcatc agcggctgtc ccatgcgcag ccgcgcgccc
      121 ggcacgatgg actcgtgcag ctcgaagccg cgcggcgcga acacgatgat cgtcgagccg
      181 tgctggaacc agcccatctc ctcgcccttg gcgaaacggg cgggatgccc ggccagcgag
      241 atctcgttcg gccccttgta gcgcaggtgc agcagcacgt cgaggaactt cagccgcagg
      301 ctggcgacca ggatcgccgc caccggcacg atggccaccg gatgcccgcc gcgcgcgagc
      361 cgcgtgcgca gcaccgcgcg ctcgttgcgg cagaacaggc gctcgacccg cttcagcgcg
      421 atcgggttga cgttccaggc gtcgccgctg tggtaggtca cgtgctccac cgcgaggtcc
      481 tgcggcgcgt ggaagcggtg gtacatcgcc gaagtcaggc gcagcgtcac gtaggtgccg
      541 tctcggtacg gcgcgacgtc gttggacggg ccgaacagct cctccatccg gtaggggaag
      601 cccttggcct ggaacacctc ggtgcccgcg gccgagccgc aggcgccgac gatggcgtcg
      661 cagggcgagg tcagcacgcc ggggtcgggg tcgaccggac gcgcgccggg cacgagctcg
      721 cgcgtgaagc agtcgtgcag gctgtcgaag cggcgcttgc gcgcgtcgct gaggtcgagg
      781 tccgtgaaca gcttccaggt cgcgatcgac aggcggcaga cgaccgggtt gcggatcttg
      841 ctgaaccagc ccatgaaccg cgtgagcgcg atgcgcggga cgcggttggt gagcaggaag
      901 ttcaggtcct cctgcaggag caggctgcgc aggaggttct tcatcgcggg cgtcggccgg
      961 acgtcgggat cgtggagaag cgctggcgca gcatctcgcg cagcaggccg cggcggatcg
     1021 ccttgttgct ctgcgccgga tcctcgcgcc accagccgtc ggcctgcatc tcgagggcgg
     1081 cggcgacgaa gcgctcggtg acggcctcga agtcctcgtc gcggtagttc aggctgaaga
     1141 tgaagcggcc ggtgccgatc cagctcagcg cgaggccgtg cgcgcgcagg tagaactgca
     1201 gcatccagtt gtagcgcgag ggcttgtcgt agagcaccgt ccagatcgac gcaaggttgg
     1261 ccacgcgcac cggcagtccg gcgccgcgca ggcggtcgtt gaagcgcgcg gtgcgcgcgt
     1321 tccagcgctc gtcgacgccg tcgtagagcg cctgcacctc gggcgtcctc aggcgctcga
     1381 ggaaggcgtt catcgcgccc atcacgtacg ggtgcgcgtt gaaggtgccg cgcgcgaagc
     1441 agatgtcggc gggacgatcc tcgcggaagc gcttcatcag gtcggcgcgg ccgcagacca
     1501 cgcccaccgg caggccgccg cccagcgtct tgccgtaggt gaccatgtcg gcctgcacgc
     1561 cgaagtactc ctgcgcgccg ccgggcgcca ggcggaagcc gacgaagatc tcgtcgaaga
     1621 tcagcacgat gccgcgcgcc gtgcagacct cgcgcagctt cctcagccaa gcggtgtagg
     1681 cggcgcggtc gaagtgcgcg ctgcggccgc tgtcgaccag cgacgagtcg cccggcgcgg
     1741 ccttgttcgg gtgcagcgcc tgcagcgggt tgaccagcac gcaggcgatg tccttgcgcg
     1801 tgcgcaggac gtgcagcgcg gcgtcgtcca tctcctcgag cgtgtagacg tcgcgcggcg
     1861 gcagcgggtt gccggggccg ggctgcacgt cctcccacca gccgtggtag gcgccgcaga
     1921 agcgcacgag gttgcgcttt cgcgtgtggt agcgcgcgag gcgcacggcc tgcatcacgg
     1981 cctcggtacc ggacatgtgg aacgagatct cgtccttgtc cgagatgcgc ttcaactcgg
     2041 cgatgttctc gagcacgcac gggtggtagg cgccgagcac cgggcccagc tcggccacgc
     2101 gtgcggcgcc ctcggcgatg cacgccttgt agaagtcgac gccgaacagg ttgacgccgt
     2161 aagagccggt gaggtcgtac aggacgttgc cgtccaggtc ctcgaccgtc acgccctgcg
     2221 acttcacgag gaagctgccg gtcttcaggt gctggcgcag gtaggggctg tactggtagg
     2281 ggacgcggta gctgccggtg aactgcaggt cggagatcgc gtcgcgcgcc tgcgccgtca
     2341 tcgcggcgct gcgcgggaag cgctcggcgt acagcgcggc caggcgcgaa aagccggcgc
     2401 ggcgacgcgc gacgacctcg gcgggcgcgt cgtcggagcc gaagaagcgc gcctcgtcgt
     2461 acgcgtagcc gggcagcagc gcggcgacgc gcttggccat gcgcgaatgt cccgccagcg
     2521 aggcgtgctt ggcgcgcgac agctccagcc ggcgcttggc cctgggcagc gtccacgcga
     2581 gcaggcccag catcacggcg gacagggcga aggtcgtatc catgggtctt cggaagttgc
     2641 agcgaacgtg cacatgtcga ggcccgcggc gacgtgcgcg tcgcggcggg cctcgtcctc
     2701 gcggcgccgt cggcgctcag gtcgcgtcgg cgtggcggcg catcagcttc agcggcgcgc
     2761 cgtggtacag catgatgtcg tggaacggat cggtgatgat cttggtcgcc cacgcgaggc
     2821 cgtggatcag gccctgggtg cgccacagct gcagcacgcg gaagatcagg cccgtgaggc
     2881 ccagcgacag ccaggcgatg cccaggtcgt gcaggaagcc gcggaagtcg acggcgggct
     2941 ggatcaggcc gaacagcgag ggctcgaacc acagcacggc ggggatcagc gcccacaccg
     3001 tcatcagcac gatcttgcgc tggatgttgt agccgacctt gatctcttcc ttgtactcgt
     3061 cggtggcctg gttgacgtgg tcgtagccgc gcggctcgaa gaagaagtgg ccggcctggc
     3121 gggtggtcat cgacacgcac cacgcgagca gcgccgcgga ggcgggatcg aagaacatca
     3181 tgccgtaggc gacgacgaag ctcaccgcgc tgacgaagtg caggctctgg ttgacgaggc
     3241 tgtggtggta gtagcggtgg tcgtcccagc gctggacgcg aaggagttca aagaagccgt
     3301 tcacggtgtt cctcgaagag gtggatggac ccggggagcg ctctgccgat gggagcggac
     3361 gcgggcagcc cggggcgtga caggagcatg accaggaaat gtgaaatcgc cgtgtcggaa
     3421 cgccggaccg aacgatgaca gcgcacgtga caggtcgtcg tcctgtcatc gaactgttgc
     3481 gggccgtcgt catgatcgcg gccatcgctg tcacgatccg gaggatcacc cgtggacctg
     3541 cccaccgcgc ctgcgcgcac cctgcggatc gcctgcgtga cggagaccta tccgccggag
     3601 gtcaacggcg tcgcggtgac gatcgcgcgc ctcgtcgagg gcctgcacgc gcgcggccac
     3661 gacctgcagc tcgtgcgccc gcgccaaggg tcggatgcgg gcgcgcccgc gccgcgcgag
     3721 cgcttcgacg aggtgctggt gcgcggcgtg ccgatcccgc gctacgccgg gctgcgcatg
     3781 gggctgccgt gccggcgcaa gctcgtgaag ctctggaagg cgcgccgccc cgatctcgtg
     3841 cacatcgcga ccgagggccc gctcggacgc tcggcgctgc aggccgcgcg cgcgctcggg
     3901 ctgccgacct gctcggactt ccgcaccaac ttccacgcct acagcacgca ctacggcatc
     3961 ggctggctcg agcgcccgat catggggtac ctgcgccgct ttcacaacgc cacgggctgc
     4021 acgatggtgc cgacggcggc gctgcaccgc gagctcgagg ccgaaggctt ccgcaacgtc
     4081 acggtggtcg cgcgcggcgt cgacaccagg cgcttcgacc cggcgcggcg cgacgaatcg
     4141 ctgcgcgcgc agtggggcgc agggcccgac cagctggtcg tcggctacat cggccgcctg
     4201 gcgccggaga agaacctggc cgtgctgctg cgcgcgttcg acgccgtgcg cgcggccgac
     4261 ccgcgtgcca agctcgtctt cgtcggcgac ggccccatgc gcgccgagct gctggcgcgc
     4321 tgccccgacg cgcacttcgc cggccagcgc accggcgacg acctcgcggc gcactacgcc
     4381 tcggccgacc tgttcctgtt cccgagcgtc accgagacct tcggcaacgt caccacggag
     4441 gcgatgtcca gcgggctggc ggtggtcgcg ttcgactatg ccgcggccgc gcgcttcatc
     4501 cgcaccgagg tcgacggcgt gctcgtgccc ttcggcgatg ccgacgcctt cgtggccgcc
     4561 gcggcacgca cggcgtccga cctcgcgcgc tgccgcacga tgggcgcgca ggcgcgcatg
     4621 acggcgcgcg ctctcgactg ggaaggcgtc gtcgcgcagt tcgagggcgt gatcgcctcg
     4681 gtgctgcgcg aggcggcgac cgcgtcggca cccgcgggac
//
LOCUS       DASVLN010000986         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_267502, whole genome shotgun sequence.
ACCESSION   DASVLN010000986 DASVLN010000000
VERSION     DASVLN010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267502"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VF453_23130"
     CDS             complement(<1..830)
                     /locus_tag="VF453_23130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX7690617.1"
                     /translation="MRASRLLSLLMLLQTRGRASAQQLAEALEVSVRTIHRDIDELSA
                     AGVPVWADRGRSGGFQLQPGWRTRVDGLTAPEAQAMFLGGLPGPASELGLGEAMASAQ
                     LKLLAALPDGWRDDARRVSARFHLDPIDWYRGPSATDHLPAVAQAVWGERRIAIRYDS
                     WKGEVERRVDPLGLVLKAGTWYLVALAGASSSAKAGPRTYRVSNVLALTPTQERFERP
                     AGFDLAAWWQASTRRFESELQRETAVLRVSPAGMKALRDLGQAVADAAAASAGRADAR
                     G"
     gene            922..1725
                     /locus_tag="VF453_23135"
     CDS             922..1725
                     /locus_tag="VF453_23135"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX7690618.1"
                     /translation="MHATTPPPDTPASAAAGEPFVPTPPVDYAPTVVELRNYLLHRGR
                     RDELIDLFEREFVEPQEACGMRILGTFRDLDDPDRFVWLRGFRDMASRPAALEAFYLG
                     PVWQRHRDQANPTMIDSDDVRLLRPLDCVRGLLGALSPRPAVGALPRRPAGVFTMTVC
                     PLRASATPEVVAAFDRHVHPWWVGTGGDLLAGFATEDAPNNFPRLPVREGEPVLAWLT
                     RFESEAAQRRHAALVESSGCLRQPAWRDHLAADAVDIRLVPTARSTLRA"
     gene            1768..2292
                     /locus_tag="VF453_23140"
     CDS             1768..2292
                     /locus_tag="VF453_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690619.1"
                     /translation="MTSTTHLPDALTAEFVGRPGDFAFLAGRWQVTNRRLRERGVGCT
                     EWNTIPAVNEGWSLMNGQISVDEITFEGQPFIGSTFRTLDVAAGRWAIYWVSSRDGRL
                     QPPVHGGWAGDRGMFIGDDRDGDRPIRVRFLWERLGADVARWSQDFAPIDAEGALAGP
                     WETNWIMDFRRLPR"
     gene            complement(2264..3451)
                     /locus_tag="VF453_23145"
     CDS             complement(2264..3451)
                     /locus_tag="VF453_23145"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7690620.1"
                     /translation="MTDPRSRNPGLDTLRAAAIALVFAYHYMVFVSGRATFGFASRIG
                     WTGVDLFFVLSGYLIANQLLGGMARGDVPSLPRFYARRALRTLPVFWLVLAAYVLFPA
                     TLGGRTPPPLWRFLTFTQNIGLAPGTAFSHAWSLCVEEQFYLVLPLVLAVATALAGGR
                     LGRRHGWWLMGALVALGVTARALLWPHWWREADAPGYMAWIYYATPCRFDEFVPGVAV
                     AMLRNFHRPAWERLVARGGLVFALGTIATGGLLVLDDRAYYVEGVGWGWFMTVFGYSL
                     LAMAFALLVVAALSPARTPLRWRVPGARTLALWSYSTYLSHKPLAFVLARALKPLALA
                     APTQAAAIALACVVAGGLLYRLVEAPFLALRDRLVPTSFRDAPAATAPGAALVSAAGG
                     GSP"
BASE COUNT          523 a         1395 c         1304 g          404 t
ORIGIN      
        1 ccccgcgcgt cggcgcgacc ggcgctcgcg gccgccgcgt cggcgaccgc ctgcccgagg
       61 tcgcgcagcg ccttcatgcc ggccggcgag acgcgcagga cggcggtctc gcgctgcagt
      121 tccgactcga agcgacgggt cgacgcctgc caccacgccg cgaggtcgaa gccggcgggc
      181 cgctcgaagc gctcctgcgt cggcgtgagc gccaggacgt tggacacgcg gtaggtgcgc
      241 ggcccggcct tggccgagga cgacgccccc gccagcgcga cgaggtacca ggtgcccgcc
      301 ttcagcacga gcccgagcgg gtcgacgcgg cgctcgacct cgcccttcca gctgtcgtag
      361 cggatcgcga tccggcgctc gccccacacc gcctgcgcca cggccggcag gtggtcggtc
      421 gccgacgggc cgcggtacca gtcgatcggg tcgagatgga agcgcgcgct gacgcgccgc
      481 gcgtcgtcgc gccagccgtc cggcagcgcc gcgagcagct tcagctgcgc cgaggccatc
      541 gcctccccca gcccgagctc ggacgccggc cccggcaggc cgccgaggaa catcgcctgc
      601 gcctcgggcg cggtcaggcc atcgacgcgc gtgcgccagc ccggctgcag ctggaagccg
      661 ccgctgcggc cgcggtcggc ccacaccggc acgccggcgg cgctcagctc gtcgatgtcg
      721 cggtggatgg tgcgcaccga cacttcgagt gcctcggcca gctgctgggc gctcgcgcgc
      781 ccgcgggtct gcaggagcat cagcagggac agcagtcgac ttgcgcgcat gggggcagtg
      841 tcgctgaaaa accatgacat gccctggcat gtttagcggg cgaacatggc gccatcgacg
      901 atccacagac ggagacgagc catgcacgcc accaccccgc cccccgacac gccggccagc
      961 gccgccgccg gcgagccctt cgtcccgacc ccgccggtcg actacgcgcc gacggtggtc
     1021 gagctgcgca actacctcct gcaccgcggc cggcgcgacg agctgatcga cctgttcgag
     1081 cgcgagttcg tcgagccgca ggaagcctgc ggcatgcgca tcctcggcac cttccgcgac
     1141 ctcgacgacc ccgaccgctt cgtctggctg cgcggcttcc gcgacatggc gtcgcggccc
     1201 gcggcgctcg aggccttcta cctcggtccg gtgtggcagc gccaccgcga ccaggccaat
     1261 cccaccatga tcgacagcga cgacgtgcgc ctgctgcgac cgctcgactg cgtgcgcggg
     1321 ctgctgggcg cgctctcgcc gcggcccgcg gtgggcgcgc tgccgcggcg ccccgccggc
     1381 gtcttcacga tgaccgtctg cccgctgcgc gcgagtgcca cgccggaggt tgtcgccgcg
     1441 ttcgaccgcc acgtccaccc gtggtgggtg ggcacgggcg gcgacctgct cgccggcttc
     1501 gccaccgagg acgcgcccaa caacttcccg cgcctgcccg tgcgcgaggg cgagcccgtg
     1561 ctggcctggc tcacgcgctt cgagagcgag gccgcgcagc gccgccatgc cgcgctcgtc
     1621 gagtcctccg gctgcctgcg ccagcccgcc tggcgcgacc acctcgccgc cgacgccgtc
     1681 gacatccgcc tcgttccgac cgcgcgctcg acgctgcgcg cctgagcccc cgacccgaca
     1741 tcccgcatca acccaaggag accccgcatg acctcgacca cgcacctgcc cgacgccctc
     1801 accgccgaat tcgtcggccg ccccggcgac ttcgccttcc tcgccggccg ctggcaggtg
     1861 accaaccgcc gcctgcgcga gcgcggcgtc ggctgcaccg agtggaacac catccccgcg
     1921 gtcaacgaag gctggtcgct gatgaacggc cagatctccg tcgacgagat cacgttcgaa
     1981 ggccagccct tcatcggcag caccttccgc acgctggacg tcgccgccgg ccgctgggcg
     2041 atctactggg tcagcagccg cgacggccgc ctgcagccgc ccgtgcatgg cggctgggcg
     2101 ggcgaccgcg ggatgttcat cggcgacgac agggacggcg accgcccgat ccgggtgcgc
     2161 ttcctgtggg agcggctggg cgccgacgtg gcgcgctggt cgcaggactt cgcgcccatc
     2221 gacgccgagg gcgcgctcgc cggcccgtgg gagacgaact ggatcatgga cttccgccgc
     2281 ctgccgcgct gaccagtgcg gcgcccggcg cggtcgcggc cggcgcgtcc cggaagctgg
     2341 tcgggacgag ccggtcgcgc agcgcgagga agggcgcctc gacgagccgg tacagcagcc
     2401 cgcctgcaac gacgcaggcg agcgcgatcg ccgcggcctg cgtcggcgcg gcgagcgcca
     2461 acggcttcag cgcgcgcgcc agcacgaacg cgagcggctt gtgcgacagg taggtcgagt
     2521 acgaccagag cgcgagcgtg cgcgcgcccg gcacgcgcca gcgcagcggc gtacgcgccg
     2581 ggctcagcgc ggcgaccacc agcagcgcga acgccatcgc cagcagcgag tacccgaata
     2641 ccgtcatgaa ccagccccag ccgacgccct cgacgtagta ggcgcgatcg tcgagcacca
     2701 gcagcccgcc cgtcgcgatc gtcccgagcg cgaagacgag cccgcctcgc gcgacgagcc
     2761 gctcccaggc cggccggtgg aagttgcgga gcatcgcgac ggcgacgccg ggaacgaact
     2821 cgtcgaagcg gcagggcgtc gcgtagtaga tccatgccat gtagcccggc gcgtcggcct
     2881 cgcgccacca gtgcggccac agcagcgcgc gggccgtgac gcccagcgcg acgagcgcgc
     2941 ccatcagcca ccagccgtgg cgccggccga gccgcccgcc cgcgagcgcc gtcgccaccg
     3001 cgagcaccag cggcagcacg aggtagaact gctcctcgac gcacagcgac cacgcgtgcg
     3061 agaacgcggt gcccggcgcg aggccgatgt tctgcgtgaa ggtcaggaat cgccacagcg
     3121 gcggcggcgt gcgcccgccc agcgtcgccg ggaacaggac gtacgccgcg agcacgagcc
     3181 agaacaccgg cagcgtgcgc aacgcgcggc gcgcgtagaa ccgcggcaac gacggcacgt
     3241 cgccgcgcgc catccccccg agcagctggt tggcgatgag atagccgctc agcacgaaga
     3301 acaggtcgac gcccgtccag ccgatgcgcg aggcgaagcc gaaggtcgcg cggccgctca
     3361 cgaagaccat gtagtggtag gcgaagacga gcgcgatggc ggcggcgcgc agcgtgtcga
     3421 ggccgggatt gcgggatcgg ggatcggtca tggggcggga gtgtcggtcg ccccggccgg
     3481 cgcgtcgcgt ggcgcagcga cgaaacgcgg aacccggggc gcggggtgcg gtcaggcgaa
     3541 gcggtcgacg agccggacga ccgtgcgcca ggcgcgcgtc gacgccgggg tgcccagcag
     3601 ccgctcgagc agcgggttgg gcgagc
//
LOCUS       DASVLN010000987         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_213408, whole genome shotgun sequence.
ACCESSION   DASVLN010000987 DASVLN010000000
VERSION     DASVLN010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213408"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VF453_23150"
     CDS             <1..705
                     /locus_tag="VF453_23150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7690621.1"
                     /translation="LGAALWVDHRARGAGEGAPAARHWQMRHAYWGPGYSDDEIEALL
                     KWAKVRYRALDDVARDTAAILAQDRIIGWFQGRMEFGPRALGARSILASPLSPSMQAR
                     LNELKDREDFRPVAPVVPVEDLGAWFTPAQANRGEAPFMLFLYGVRPEKATLIPAVCH
                     ADRTARVQAVARETNPRYHDTLKAFEALTGAPVLVNTSFNVRGEPVVCSPRDALSAFF
                     STPLDALVIGRFLVEK"
     gene            705..1727
                     /locus_tag="VF453_23155"
     CDS             705..1727
                     /locus_tag="VF453_23155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7690622.1"
                     /translation="MLDTADGTQAAVPRISVVMPTFRRPELLARSLRALMAQSVPGDA
                     FEIVVVDDGRTDDTHACCARLAAQSLAAGGPVIRYLRPDGGHGPAAARNRGWQAAAGA
                     LIAFTDDDTVPDPDWLRQGEAAMAAPRRVACWGRVHVPMPERVTDNARNTAGLENAVF
                     VTANAFVLKAALSRVGGFDERYLRAWREDTDLYFALLQAFPEPGAVAAAPEALVLHPV
                     RDAKFLVSIGQQANMAFDALLFKKYPTLYDRHCGIRHVPALYLAVVLATLAAVLALPF
                     SPAAAAVAAAVAVGGILLFALRRLRGLAWSPRQVADMLISSVAIPYVSLYWRLVGALR
                     WRVAFC"
BASE COUNT          218 a          677 c          668 g          275 t
ORIGIN      
        1 ctcggcgcgg cgctgtgggt cgaccaccgc gcgcgcggcg ccggggaggg cgcgccggcc
       61 gcgcggcact ggcagatgcg ccatgcgtac tggggcccgg ggtattcgga cgacgagatc
      121 gaggcgctgc tgaagtgggc caaggtgcgc taccgcgcgc tcgacgacgt cgcgcgcgac
      181 accgccgcca tcctcgcgca ggacaggatc atcggctggt tccagggccg catggagttc
      241 gggccgcggg cgttgggcgc acgctccatc cttgcctcgc cgctcagccc gtcgatgcag
      301 gcgcgcctga acgagctcaa ggatcgcgag gacttccgcc ccgtcgcccc ggtggtgccg
      361 gtcgaggatc tcggcgcctg gttcacgccc gcgcaggcca accgcggcga ggcgccgttc
      421 atgctcttcc tctacggcgt gcggccggag aaggcgacgc tgatcccggc cgtctgccac
      481 gccgaccgca cggcgcgggt gcaggcggtg gcgcgcgaga ccaacccgcg ctaccacgac
      541 acgctcaagg ccttcgaggc gctcaccggc gcgccggtgc tggtcaacac ctcgttcaac
      601 gtgcgcgggg agccggtggt gtgctcgccc cgcgatgcgc tctcggcgtt cttctcgacg
      661 ccgctggacg cgctggtcat cggccggttc ctggtggaga agtgatgctc gataccgcag
      721 acggtaccca ggccgcggtg ccgcgcatca gcgtcgtgat gccgacgttc cgccggcccg
      781 agctcctcgc ccgcagcctg cgggccctga tggcgcagtc cgttccgggc gatgccttcg
      841 agatcgtggt ggtcgacgac ggccgcaccg acgacacgca cgcctgctgc gcacggctgg
      901 cggcgcagag cctggccgcg ggcggcccgg tgatccgcta cctgcggccc gacggcggcc
      961 acgggccggc ggccgcgcgc aatcgcggct ggcaggcggc cgcgggcgcg ctcatcgcgt
     1021 tcaccgacga cgacaccgtc cccgatcccg actggctgcg ccagggcgag gccgcgatgg
     1081 cagcgccgcg ccgcgtcgcc tgctgggggc gcgtgcacgt gccgatgccc gagcgcgtga
     1141 ccgacaacgc gcgcaacacg gcgggactgg agaacgccgt cttcgtcacc gccaacgcgt
     1201 tcgtgctgaa ggccgcgctg tcgcgcgtcg gcggcttcga cgagcgctac ctgcgcgcct
     1261 ggcgcgagga cacggatctc tacttcgcgc tgctgcaggc gtttcccgag ccgggcgcgg
     1321 tcgccgcggc gcccgaggcg ctcgtgctgc atccggtgcg cgacgcgaag ttcctcgtga
     1381 gcatcggcca gcaggccaac atggccttcg acgcgctgct gttcaagaaa taccccaccc
     1441 tgtacgaccg ccactgcggg atccgccacg tgcccgcgct ctacctcgcg gtcgtgctgg
     1501 cgacgctggc cgcggtgctc gcgctgccgt tctcgccggc ggcggccgcg gtcgcggcgg
     1561 ccgtcgcggt cggcggcatc ctgctgttcg cgctccggcg cctgcgcggc ctcgcctggt
     1621 cgccgcggca ggtggccgac atgctgatca gctcggtggc gatcccctac gtctcgctgt
     1681 actggaggct cgtcggcgcg ctgcgctggc gggtggcgtt ctgctgatgg ccccgctgca
     1741 cgagatccgc tcgatcctcg tattccgcgc gctggtgctc ggcgactggt tgtgcgcgac
     1801 gcgaagttcc tcgtgagcat cggccagcag gccaacat
//
LOCUS       DASVLN010000988         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_107195, whole genome shotgun sequence.
ACCESSION   DASVLN010000988 DASVLN010000000
VERSION     DASVLN010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107195"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1556)
                     /locus_tag="VF453_23160"
                     /pseudo
     CDS             complement(16..1556)
                     /locus_tag="VF453_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874585.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
BASE COUNT          203 a          526 c          617 g          210 t
ORIGIN      
        1 cgcgggcagc cccgctcatc gcccggcgcc cgccccgccc ggcagcgcgt aggcgatcac
       61 cgcgtcgccg ggcttggtgc cggtcgagcc gtggccgccc gcgaccacca gcacgacctg
      121 gcggccgttg gcgtcgaggt aggtcatcgg cgtcgcctgg ccgccggcgg gcaggcgcga
      181 ctgccacagc tggcggccgt cgtcgaggtc gtaggcgcgc acgtagtcgt ccatcgtccc
      241 ggagaggaag gccagcccgc cgcgcgtgac gaccgggccg ccgaggtcgg gcacgcccat
      301 cttcagcggg atcgggatcg gcgagaggtc gcgcacggtg ccgttgcgat ggcgccaggc
      361 gatgcggccg gtgcgcaggt cgaccccggc gacgtagccc cacggcggcg cctggcaagg
      421 gatgcccagc ggcgagagga aggcgccgag cttgatcgcg tagggcgcgc cgaagttctc
      481 gttgagggcc ggcaggacgc cgtgcggcgg cgtcccgtgc gcctgcacga gcagcgactt
      541 cgcgttcgtg cgcggcacca gctgcgacgt gaaggcgagg taggccggcg tcgtgaacgc
      601 gacctgccgc tccgggtcga ccgcgatccc gccccagttg aagacgccga agttgcccgg
      661 gtagatgagc gagccctgcg tcgacggcgg cgtgaagcgg ccgccgtagc gcagcttgcg
      721 gaaggcgatg cggcacagcg tctggtcgaa tatcgtcgcg ccccacatgt cggcttcagt
      781 gagcggcggc gggtcgaacg acagctgcga gcgcggctgc gtcggcgcgc tgtggtcgtc
      841 gggcgctgcg ccggtgggcg cgggctgctc gctgaccggc agcaccggct cgccggtgcg
      901 gcggtcgagc acgaacagct cgccctgctt ggtcggctgc acgagcgccg gcacgcgcgc
      961 gccgtggacg tccaggtcga ccagcgtcgg ctgcgcgggg acgtcgtagt cccacaggtc
     1021 gtggtgcacg gtctggaaga cccagcgcag cttgccggtc gccaggtcca gcgcgacgac
     1081 cgaggacgag acgcgctcgg tggtcgccgg acgcctggcg ccgtactggt cgggcggctg
     1141 gttgcccatc gggaagtagg ccaggcccag cgactcgtcg gcgctcgcga tcgaccagct
     1201 gttgggcgag ttggcggtgt aggtgcggcc ggcgccgatg ggcgtcgtcg cctccgggtt
     1261 gcccgagtcc cagttccaca ccagggcacc ggtgtcgatg tcgtaggcgc ggatgacgcc
     1321 ggactcctcg tgcgtcgagt agttgtccag caccgtgccg ccgacgatca cgaggtggcg
     1381 ggtgaccagc ggcggcgagg tcgagtagta cgagcccggc agcaggttgg gctgttggcg
     1441 gcgaggtcga cctggcccgt gccgccgccg aagcgggtgc agaccgcgcc cgactccggg
     1501 tcgagcgcga tcaggcgggc gtcggcggtc gccatgaaca gcttggcgcc gcagtc
//
LOCUS       DASVLN010000989         5094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_267623, whole genome shotgun sequence.
ACCESSION   DASVLN010000989 DASVLN010000000
VERSION     DASVLN010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267623"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VF453_23165"
     CDS             <1..1191
                     /locus_tag="VF453_23165"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX7690623.1"
                     /translation="LARWRGGAGAWAWVEAFCEASAVGAVADWFAVVALFRHPLGVPL
                     PHTAIIPRGKERLADGLAGFVRDHFLEPAALVERLQVFDPARRLADWLAEPERVREWV
                     GEGRRWALKAVGLFDDSRMRRATMELVVAQLRKWDAAATAAEVLALLTQGGRHHELLD
                     AALVKVGDLLAEDEVKARVSALMVRHARREWPKIIGMVELVTPAGRIADNLADKLSAS
                     VLGELREVMAQPDHPVRLRYERWLEEFIARLREDEALVASFERVKERMITDPALQAYV
                     AGVWGDIKRLLREDLADEHSAIAGHVEAALHDVGERLRHDESLRAALNEHLLSAAGQL
                     AANLRGGVTDHIAQTIKAWDDRRLVRELELGVGRDLQFIRINGTVVGGAVGLALHALR
                     LAIG"
     gene            1265..2803
                     /locus_tag="VF453_23170"
     CDS             1265..2803
                     /locus_tag="VF453_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX7690624.1"
                     /translation="MKLKIKLPLAFSGVVAALMGAALVGILGLGSAISTYGTDVAAGH
                     ADERAVDQMLVAFKVQVQEWKDTLLRGKDPQALDKHWTAFAKQEAAVDEAARTLLAQL
                     PPGESRDLVGRFAAEHVRMGQGYRQGFDAFKAAGFDPTAGDVAVKGVDREPARLLAEA
                     AKRIAAHSAEVSARAAVEAHRATILSVVLMVVGGVVGLAVGVAFSRRITKPVEKALEF
                     SQAVAGGDLATRVRPRGDDEIADLLRSLLAMQDSLSAVVRQVRRDAEGVAAASTQIAN
                     GGADLSQRTEEQAAALQQAASAMDELSTTVRQNADNAGEADQLARGASDVAQRGGAVV
                     GEVVQTMRGIHESSRRVADIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASE
                     VRSLAQRSAEAAREIKALISASVERVEQGSALVDQAGATMTEVVAAIGRVAGLMGEIS
                     AASSEQSTGVGHIGQAIAHMDDATQHNAALVEESAAAAASLKDQAHGLVQAVAVFRLA
                     PAIA"
     gene            2853..3305
                     /locus_tag="VF453_23175"
     CDS             2853..3305
                     /locus_tag="VF453_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7690625.1"
                     /translation="MPRTANTAAPTRADAPLTKADFEALSEFRFQIRRFERFSEDATL
                     AEGIQPLQYLLLLHVKGYPGREWATVGELAERLQAKPHGVVALVTRCAEHGFVERRQS
                     VHDRRQVEVHLRPAGERVLAHLAAMHRAELQSLGDVFTLRSLGAGGPQ"
     gene            3302..>5094
                     /locus_tag="VF453_23180"
     CDS             3302..>5094
                     /locus_tag="VF453_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX7690626.1"
                     /translation="MSAGESAIEDLWQDNDPRRDFAINGRLPGIALLAAFVGAVSTAA
                     AWLLLHLIRLFTNLFFFQTVSTQLVSPALHQLGPAVILVPVVGGLVIGLMARYGTEKI
                     RGHGIPEAIEAILFGRSRMSVKVAILKPLSSGIAIGSGGPFGAEGPIIMTGGAVGSIA
                     GQFFRITGAERKTLLVAGAVAGMTAVFGTPIAALLLAVELLLFELRPRSLLPSAIACC
                     VAAALRPLVLDAGPLFPLQTARADLATLAACVPAGLATGALAWGVSTSLYKVEDAFHR
                     LPVHWMWWPAIGGLAVGIGGWIEPRAFGVGYDVIGDLLANRLAVSVVVALIACKAAMW
                     VVALGSGTSGGVLAPLLMMGAGLGVVLGPWLPGGQPQLWPLVFMAATLGGMMRAPIMA
                     AMFAFELTHDVEALLPLLVATAVAYGFTVLVMPRSILTEKIARRGRHIYREYGIDPLE
                     RHHVAEVMTRDVVGIPAGMELAQALERFFGPDQRHRAFPVMRGEAFVGMIDRETVQAA
                     AGRAEARSVGDLFGANVPMMALPEETCREVATRLAVHGLERVPVVTDARTRRLIGLVS
                     RSDLVKPSLGLFEEEHQAERLRPPPLRTLARR"
BASE COUNT          648 a         1855 c         1895 g          696 t
ORIGIN      
        1 ctcgcgcgct ggcgcggcgg cgcgggcgcg tgggcctggg tcgaggcgtt ctgcgaggct
       61 tccgccgtgg gcgcggtggc cgactggttc gcggtcgtcg cgctgttccg ccatccgctg
      121 ggcgtgccgc tgccgcacac ggccatcatc ccgcgcggca aggagcgcct ggccgacggc
      181 ctcgccggct tcgtccggga ccacttcctc gagccggccg cccttgtcga gcgcctgcag
      241 gtcttcgatc ccgcgcgccg gctcgcggac tggctggccg agcccgagcg cgtgcgcgag
      301 tgggtcggcg agggccggcg ctgggcgctc aaggcggtcg ggctgttcga cgacagccgc
      361 atgcggcgcg cgacgatgga gctggtcgtc gcccagctgc gcaagtggga cgccgccgcc
      421 accgcggccg aggtgctcgc gctgctgacg cagggcggcc gccaccacga gctgctcgac
      481 gcggcgctcg tgaaggtggg cgacctgctg gccgaggacg aggtgaaggc gcgcgtgtcg
      541 gcgctgatgg tgcgccacgc gcgcagggag tggcccaaga tcatcggcat ggtcgagctc
      601 gtcacgcccg ccggccgcat cgccgacaac ctggccgaca agctcagcgc ctccgtgctc
      661 ggcgagctgc gcgaggtgat ggcgcagccg gaccacccgg tgcgcctgcg ctacgagcgc
      721 tggctcgagg agttcatcgc ccgcctgcgc gaggacgagg cgctcgtggc ctcgttcgag
      781 cgcgtgaagg agcgcatgat caccgacccg gcgctgcagg cctatgtcgc gggcgtgtgg
      841 ggcgacatca agcgcctgct gcgcgaggat ctcgccgacg agcactccgc catcgccggc
      901 cacgtcgagg ccgcgctgca cgacgtcggc gagcgcctgc gccacgacga gagcctgcgc
      961 gccgcgttga acgagcacct gctgtcggcc gcgggccagc tcgcggccaa cctgcgcggc
     1021 ggcgtcaccg accacatcgc ccagaccatc aaggcctggg acgaccgccg cctcgtgcgc
     1081 gagctggagc tgggcgtcgg gcgcgacctg cagttcatcc gcatcaacgg cacggtggtc
     1141 ggcggcgccg tcggcctcgc gttgcacgcc ctgcggctcg cgatcggctg aaggcgcgcc
     1201 gcgcgcctcc tcgaaacggc gtcgagattt ctaaagtcgg gacaaaccct gtcgaaagcc
     1261 agtcatgaaa ctcaagatca agctgccgct cgcgttcagc ggcgtcgtcg ccgccctcat
     1321 gggcgccgcc ctcgtgggca tcctgggcct gggctcggcc atctccacct acggcacgga
     1381 tgtcgcggcc gggcacgccg acgagcgcgc ggtcgaccag atgctggtcg cgttcaaggt
     1441 gcaggtgcag gaatggaagg acacgctcct gcgcggcaag gatccgcagg cgctggacaa
     1501 gcactggacc gcgttcgcga agcaggaggc cgcggtcgac gaggcggcgc gcacgctgct
     1561 cgcgcagctg ccgccgggcg agagccgcga cctggtcggc aggttcgcgg ccgaacacgt
     1621 gcgcatgggc cagggctacc gccagggctt cgacgcgttc aaggccgcgg gcttcgatcc
     1681 gaccgcgggc gacgtcgccg tcaagggcgt cgaccgcgag ccggcgcgcc tgctggccga
     1741 ggcggccaag cggatcgccg cgcacagcgc cgaggtgtcg gcgcgcgcgg ccgtcgaagc
     1801 gcaccgcgcg acgatcttga gcgtcgtgct gatggtggtc ggcggcgtcg tcggcctggc
     1861 ggtgggcgtg gccttcagcc gccgcatcac gaagccggtc gagaaggcgc tcgagttcag
     1921 ccaggccgta gccggcggcg acctcgcgac gcgggttcgc ccgcgcggcg acgacgagat
     1981 cgccgacctg ctgcgctcgc tgctggcgat gcaggacagc ctgtccgcgg tcgtgcgcca
     2041 ggtgcgccgc gacgccgagg gcgtggccgc cgcgtcgacg cagatcgcca acggcggcgc
     2101 cgacctgagc cagcgcacgg aggaacaggc cgccgcgctg cagcaggccg cctcggcgat
     2161 ggacgagctc tcgaccaccg tgcgccagaa cgccgacaac gccggcgagg ccgaccagct
     2221 cgcgcgcggc gccagcgacg tcgcgcagcg cggcggcgcg gtcgtcggcg aggtcgtgca
     2281 gaccatgcgc ggcatccacg agagctcgcg ccgcgtcgcc gacatcatcg ccgtcatcga
     2341 cggcatcgcg ttccagacca acatcctcgc gctcaacgcc gcggtcgagg ctgcacgagc
     2401 gggtgagcag ggccgcggct tcgccgtcgt ggcgtccgaa gtgcgctcgc tggcgcagcg
     2461 ctcggcggag gcggcgcgcg agatcaaggc gctgatctcg gccagcgtgg agcgcgtcga
     2521 gcagggcagc gcgctggtcg accaggccgg cgcgacgatg accgaggtgg tcgccgcgat
     2581 cggccgcgtc gccggcctga tgggcgagat cagcgccgcc agcagcgagc agagcacggg
     2641 cgtcggccac atcggccagg cgatcgcgca catggacgac gcgacccagc acaacgccgc
     2701 gctggtcgag gagagcgccg ccgcggccgc gagcctgaag gaccaggccc acggcctcgt
     2761 gcaggcggtg gcggtgttcc gcctcgcgcc ggccatcgcc tgagtcgcgg gccattccgg
     2821 atggttatgt cacaacgtga cataatgtcc ggatgccccg cactgccaac acggccgccc
     2881 cgacgcgcgc cgacgccccg ctgaccaagg ccgacttcga ggccctgtcc gagttccgct
     2941 tccagatccg ccgcttcgag cgcttctccg aggacgcgac gctcgccgag ggcatccagc
     3001 ccctgcagta cctgctgctg ctgcacgtca agggctatcc gggccgcgag tgggcgaccg
     3061 tcggcgagct cgccgagcgc ctgcaggcca agccgcacgg cgtcgtcgcg ctcgtgacgc
     3121 gctgcgcgga gcacggcttc gtcgagcgcc gccagagcgt ccacgaccgc cgccaggtcg
     3181 aggtccacct gcggccggcc ggcgagcgcg tgctggcgca cctggccgcg atgcaccgcg
     3241 ccgagctgca gtccctcggg gacgtgttca cgctgcgatc cctcggcgcc ggaggcccgc
     3301 aatgagcgcc ggcgaatccg cgatcgagga cctctggcag gacaacgacc cgcggcgcga
     3361 cttcgccatc aacggccgcc tgccgggcat cgcgctgctc gcggccttcg tcggcgcggt
     3421 gagcaccgcc gcggcgtggc tgttgctgca cctgatccgg ctgttcacca acctgttctt
     3481 cttccagacc gtctcgacgc agctcgtgtc gccggccctg caccagctcg ggcccgcggt
     3541 catcctcgtg ccggtcgtcg gggggctggt catcggcctg atggcgcgct acggcaccga
     3601 gaagatccgc ggccacggca ttcccgaggc gatcgaggcg atcctgttcg gccgcagccg
     3661 gatgtcggtc aaggtcgcga tcctgaagcc gctgtcctcc ggcatcgcga tcggcagcgg
     3721 cggcccgttc ggcgccgagg gcccgatcat catgacgggc ggcgcggtcg gctcgatcgc
     3781 ggggcagttc ttccgcatca cgggcgccga gcgcaagacg ctgctcgtgg ccggcgctgt
     3841 ggcgggcatg acggcggtct tcggcacgcc gatcgcggcg ctgctgctgg ccgtcgagct
     3901 gctgctgttc gagctgcggc cgcgcagcct gctgccctcg gccatcgcct gctgcgtcgc
     3961 cgctgcgctg cggccgctgg tgctggacgc cggcccgctg ttcccgctgc agaccgcccg
     4021 cgccgacctc gcgaccctgg ccgcctgcgt gcccgcgggc ctggcgaccg gcgcgctcgc
     4081 ctggggcgtg tcgacctcgc tgtacaaggt cgaggacgcg ttccaccggc tgccggtgca
     4141 ctggatgtgg tggccggcga tcggcggcct ggcggtcggc atcggcggct ggatcgagcc
     4201 gcgcgccttc ggcgtcggct acgacgtgat cggcgacctg ctggccaacc ggctcgcggt
     4261 ctcggtggtg gtcgcgctga tcgcctgcaa ggcggcgatg tgggtcgtgg cgctcggctc
     4321 gggcacgtcg ggcggcgtgc tcgcgccgct gctcatgatg ggcgcgggcc tgggagtcgt
     4381 gctcgggccc tggctgccgg gagggcagcc gcagctgtgg ccgctcgtgt tcatggccgc
     4441 gacgctgggc ggcatgatgc gcgcgccgat catggcggcg atgttcgcgt tcgagctcac
     4501 gcacgacgtc gaggccctgc tgccgctgct ggtcgcgacc gcggtggcct acggcttcac
     4561 ggtgctcgtg atgccgcgct ccatcctcac cgagaagatc gcgcgccgcg gccgccacat
     4621 ctaccgcgag tacggcatcg acccgctcga gcgccaccac gtcgccgagg tgatgacgcg
     4681 agacgtcgtg ggcattcccg ccggcatgga gctggcgcag gcgctggagc gcttcttcgg
     4741 ccccgaccag cgccaccgcg cgttcccggt catgcgcggc gaggccttcg tcggcatgat
     4801 cgatcgcgag accgtgcagg cggcggccgg gcgcgccgag gcgcgctcgg tcggcgacct
     4861 gttcggcgcg aacgtgccga tgatggcgct gcccgaggag acctgccgcg aggtcgcgac
     4921 ccgcctggcc gtccacggcc tggagcgcgt gcccgtcgtc accgacgcgc gcacgcgccg
     4981 gctcatcggc ctcgtgagcc gcagcgacct cgtcaagccc tcgctcggcc tgttcgagga
     5041 ggagcaccag gccgagcgcc tgcgcccgcc gccgctgcgc acgctggcgc ggcg
//
LOCUS       DASVLN010000990         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_374924, whole genome shotgun sequence.
ACCESSION   DASVLN010000990 DASVLN010000000
VERSION     DASVLN010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374924"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VF453_23185"
     CDS             <1..221
                     /locus_tag="VF453_23185"
                     /inference="COORDINATES: protein motif:HMM:NF021035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690627.1"
                     /translation="VQRAAADIEAARAAVRRNEQRIEGFREEIAKLLRDRAAAQDEQA
                     DEEAEEAAISRFVARVRRARAQAEVSHG"
     gene            214..1065
                     /gene="sctT"
                     /locus_tag="VF453_23190"
     CDS             214..1065
                     /gene="sctT"
                     /locus_tag="VF453_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HEX7690628.1"
                     /translation="MDSIEGLSQAVAGVSGSLQDLLLAVSLCSLRLLAAFALLPPMGQ
                     KFLTGGLRNGLTTMLAIYLAFGVPLEAVHRLDGLALAGHAAKEAGIGLVLGFAASKVF
                     WIAESVGALMDTQAGFNNVQMSNPLSGQQSTPVSDLLLHVVVVVFFSMGGMLVFLGAV
                     FESFHVWPLLSPLPRVDRLPDLFIVGETDSLMTGIVKFAAPVLLVLMLIDIGFGLVTR
                     SSSKLEVSTLSQPVKGAVTVLMLALLLGTLVSQVRGMLLPTGLLEHVQSAVPATGAAS
                     SPLRVSR"
     gene            1170..>1641
                     /locus_tag="VF453_23195"
     CDS             1170..>1641
                     /locus_tag="VF453_23195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690629.1"
                     /translation="MRIIMKPSNIVAGLATPGLLGGAGLLPPGGGMHGLVQPGGLHGV
                     GHGLALPHGGAGVLDDHHGALMNSLEGMHIPMHQSPQARLEAAEGVNDSLRQALGALM
                     GGAQGVGQHHHHHHHGRVEGDGNPVDELFPKLAGARALAGAVGAGGAAAAGGAGA"
BASE COUNT          203 a          570 c          611 g          257 t
ORIGIN      
        1 cggtgcagcg cgcggccgcg gacatcgagg ccgcccgcgc cgccgtgcgc cgcaacgagc
       61 agcgcatcga gggcttccgc gaggagatcg ccaagctgct gcgcgatcgc gccgcggcgc
      121 aggacgagca ggccgacgag gaggccgagg aagcggcgat ctcccgcttc gtcgcgcgcg
      181 tgcggcgcgc gcgcgcgcag gccgaggtgt cgcatggata gcatcgaggg cctctcgcag
      241 gccgtggccg gcgtctccgg ctcgctgcag gacctgctgc tggcggtgtc gctgtgcagc
      301 ctgcgcctgc tggccgcgtt cgcgctgctg ccgccgatgg ggcagaagtt cctcaccggc
      361 gggctgcgca acggcctgac gacgatgctg gcgatctacc tggctttcgg cgtcccgctg
      421 gaggcggtgc accggctcga cggcctcgcg ctcgcgggcc atgccgccaa ggaggcgggc
      481 atcggcctgg tgctcgggtt cgccgcgtcc aaggtgttct ggatcgccga gagcgtgggc
      541 gcgctgatgg acacccaggc gggcttcaac aacgtgcaga tgtccaatcc gctcagcggc
      601 cagcagagca cgcccgtgtc cgacctcctg ctgcacgtgg tcgtggtcgt cttcttcagc
      661 atgggcggga tgctggtgtt cctcggcgcg gtcttcgagt cgttccacgt gtggccgctg
      721 ctgtcgccgc tgccgcgggt cgaccggctg cccgacctgt tcatcgtggg cgagacggac
      781 tcgctcatga ccggcatcgt caagttcgcg gcccccgtgc tgctcgtgct gatgctgatc
      841 gacatcggct tcgggctggt gacgcgctcg tccagcaagc tcgaggtctc cacgctgagc
      901 cagccggtca agggcgccgt gacggtgctg atgctggcgc tgctgctcgg cacgctggtg
      961 tcgcaagtgc gcgggatgct gctgcccacg ggactgctcg agcacgtgca gtcggcggtg
     1021 cccgcgacgg gcgcggcgtc ctccccgctt cgcgtttcgc gctgagcctt cggcggcgcg
     1081 gcacgccgcg gcgaggcgct gtcctccaat ggacgaaggc acgtcttcgc gcatcacgcg
     1141 gggtcgggtc gcacccatgt tctttccaag tgaggatcat catgaaacct tcgaacatcg
     1201 ttgccgggct cgcgacgccc ggtctcctcg gcggcgcagg cctgcttcct ccgggcggcg
     1261 gcatgcatgg cctggtccag cctggcggac ttcacggcgt cgggcacggc ctggcgctgc
     1321 cgcacggcgg cgccggcgtc ctcgacgacc accacggggc gctcatgaat tcgctggagg
     1381 ggatgcacat cccgatgcac cagtccccgc aggcacggct ggaagcggcg gagggggtca
     1441 acgacagcct gcgccaggcc ctcggtgcgc tcatgggcgg cgcgcagggc gtcggccagc
     1501 accatcatca tcaccaccac ggcagggtgg agggcgacgg caacccggtc gacgagctgt
     1561 tcccgaaact ggcgggcgcc cgtgcgctgg cgggcgccgt cggcgccggc ggtgcggcag
     1621 cggccggcgg cgcgggcgcc g
//
LOCUS       DASVLN010000991         5319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_267724, whole genome shotgun sequence.
ACCESSION   DASVLN010000991 DASVLN010000000
VERSION     DASVLN010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267724"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /gene="hemH"
                     /locus_tag="VF453_23200"
     CDS             complement(<1..1008)
                     /gene="hemH"
                     /locus_tag="VF453_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX7690630.1"
                     /translation="MSEATAFRHDRPDRTGVLFVNLGTPEAPTPAATRAYLAQFLSDP
                     RVVEIPRLAWWPILHGIVLRTRPAKSARKYAQVWMPEGSPLAVWTARQAALLRGYLGE
                     RGAAVEVRWAMRYGKPTIAAELDALCAAGATRILVFPAYPQYSAATTASVLDDVAAWM
                     HRTRRVPELRFVNHYHDEPTYIEALAAQVRRHWQREGRGRVLVLSFHGMPARTLALGD
                     PYHCECRKTGRLLAQALGLKDDEWRLTFQSRFGRAKWLEPATDATLAGLARAGAGVDV
                     LCPGFAADCLETLEEIAIEGRASFLAAGGREFHYLPCLNDSPEGMRALAAVALRQLQG
                     WP"
     gene            complement(1079..2062)
                     /locus_tag="VF453_23205"
     CDS             complement(1079..2062)
                     /locus_tag="VF453_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7690631.1"
                     /translation="MSAIQRRAVVFADLRGSTALYELIGNADASAVVTETVDAIARRV
                     PATGGVLVKTLGDGLMAVFPTAAQAVQAAEQMHEELDVVMARREALTGFHDARLQLQV
                     CIALGETVEVSGDYFGDAVNVAARLLDHAGDNETLVTAEVLRALPIEQRDIFRSLDLV
                     RLRGRVEPVEVHLLSRRGMDTAPTLVGGAALIAGPESVVLTWGNLVRRFTAADMPVII
                     GRAAAADMQVDDARVSRSHCRIDLVGATLQLSDLSINGTFVRFASEDEIVSLRRGSCT
                     LHGSGEVGLSGSPEDPGVAAMQFEVLHAPVRQLADEDMIPTMPPPLVPRRK"
     gene            complement(2153..4459)
                     /gene="rpoD"
                     /locus_tag="VF453_23210"
     CDS             complement(2153..4459)
                     /gene="rpoD"
                     /locus_tag="VF453_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX7690632.1"
                     /translation="MTAKKSADTAKTTTPAAKTAAADKPVEKKAVSAAAAPKAAARST
                     TATTATKAADKPAPAAKTAAAKAAKAPAAKTTAAAAKKGKKDEPKAPVFEDDADVEAD
                     IEGDAEAEVEAADEGGEEVGEVKAAKAKPLRMKVSRAKERALMREFGLDETQLTEEEV
                     AKRRQELKTLIKMGKTRGFLTHQEINDHLPEKLVDNEILEAIVSMLNDMGIAVYEQAP
                     DAATLLISGSAQTGATEEEAEEAAEAALSTVDSEFGRTTDPVRMYMREMGSVELLTRE
                     GEIEIAKRIEGGLQAMMQAISASPTTIAEILALAERIEKGEMQISEAVDGFVSDDEAD
                     DYVAEEDFDEFDEEDDDDGAGGSKALTKKLEELKAQALTKFAELSTHFDKMRKAFEKE
                     GYRSPAYDKAQEALTGDLMKIRFTVKTIERLCSILRGQVDDVRRYEREIRKIVVDKCG
                     MPQEHFIKTFPTNSLNLHWAEKEVAGNKGYSAVMSRNLPAIQELQQKLIDLQARAVVP
                     IEHLKEINRKMNEGEKASRDAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGL
                     MKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQH
                     LQEFGFEPDAPTLAEKMEMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDTNN
                     LAPVEAAMHAGLRDVVKDILDSLTPREAKVLRMRFGIEMSTDHTLEEVGKQFDVTRER
                     IRQIEAKAIRKLKHPSRSDKLRTYLDNL"
     gene            complement(4748..>5319)
                     /locus_tag="VF453_23215"
     CDS             complement(4748..>5319)
                     /locus_tag="VF453_23215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690633.1"
                     /translation="PRGRDRPWEPRGGGRGRFGGRFAARDREEAERAELLRLPRAAPK
                     TPSARIVEILFGHPEFWDELSASEHELLHALPAPYGPLVAWLERDVAEHGPRPWAILR
                     LALADDPSLDEATRAAADGDAHPDARHVDFRRAVDQVLERLLNARSLELSRSISTPDD
                     LRRYQLVFEHWKEVKERLSRLASQDDSEV"
BASE COUNT          674 a         1818 c         1912 g          915 t
ORIGIN      
        1 gggccagccc tggagctggc gcagcgcgac ggcggccagc gcgcgcatgc cctcggggct
       61 gtcgttgagg cagggcaggt agtggaactc gcggccgccg gcggccagga agcttgcgcg
      121 gccctcgatc gcgatctcct cgagcgtctc gaggcagtcg gccgcgaagc cggggcacaa
      181 cacgtccacc ccggcgcccg cgcgcgcgag cccggcgagc gtcgcgtcgg tcgcgggctc
      241 cagccatttc gcgcggccga agcggctctg gaaggtcagg cgccactcgt cgtccttcag
      301 gccgagcgcc tgcgcgagca gccggcccgt cttgcggcat tcgcagtggt acgggtcgcc
      361 gagcgccagc gtgcgcgccg gcatgccgtg gaagctgagc acgagcacgc ggccacgacc
      421 ctcgcgctgc cagtggcggc ggacctgcgc cgccagcgcc tcgatgtagg tgggctcgtc
      481 atggtagtgg ttgacgaagc gcagctcggg cacgcggcgc gtgcggtgca tccaggcggc
      541 gacgtcgtcg aggacgctgg ccgtggtcgc ggccgagtac tgcggatagg ccgggaagac
      601 gaggatgcgc gtcgcccccg cagcgcagag ggcgtcgagc tccgccgcga tcgtgggctt
      661 gccgtagcgc attgcccagc gcacctcgac cgcggcgccg cgctcgccga ggtagccgcg
      721 cagcagcgcc gcctgcctgg ccgtccagac ggcgagcggc gagccctcgg gcatccagac
      781 ctgggcgtac ttgcgcgccg acttcgccgg ccgcgtgcgc agcacgatgc cgtgaaggat
      841 cggccaccac gcgagccgcg ggatctcgac gacgcgggga tcggacagga actgcgcgag
      901 ataggcgcgc gtggccgcgg gcgtcggcgc ctccggcgtg cccaggttga cgaacagcac
      961 gccggtcctg tccggccggt cgtggcggaa tgcagtcgct tcgctcatcg tgaggggaac
     1021 cgcgtcgcgg ggggaacgct cgtcgggaac ggtcgcgccg cgcggtgcac ggcgcccttc
     1081 atttgcggcg cgggaccagc ggcggcggca tcgtcgggat catgtcctcg tcggccagtt
     1141 gccgcacggg cgcgtgcagc acctcgaact gcatcgccgc gacgcccgga tcctccggcg
     1201 agcccgacag cccgacctcg ccgctgccgt gcagcgtgca gctaccgcgg cgcaggctca
     1261 cgatctcgtc ctcgctcgcg aagcgcacga aggtgccgtt gatgctcagg tcggacagct
     1321 gcagcgtcgc gccgacgagg tcgatgcgac agtgcgagcg cgagacgcgc gcatcgtcca
     1381 cctgcatgtc ggccgccgcc gcgcggccga tgatcacggg catgtcggcg gccgtgaagc
     1441 ggcgcaccag gttgccccag gtgaggacca ccgactcggg gcccgcgatc agcgcggcgc
     1501 cgccgaccag cgtcggcgcc gtgtccatgc cgcggcgcga cagcaggtgt acttcgaccg
     1561 gctcgacacg cccgcgcagc cgcacgaggt cgaggctgcg gaagatgtcg cgctgctcga
     1621 tcggcagcgc gcgcagcacc tcggccgtga cgagcgtctc gttgtcgccg gcgtggtcga
     1681 gcagccgcgc cgccacgttg acggcgtcgc cgaagtagtc gccgctgacc tcgaccgtct
     1741 cgcccagcgc gatgcacacc tgcagctgca gccgggcgtc gtggaagccg gtgagcgcct
     1801 cgcggcgcgc catcacgacg tcgagttcct cgtgcatctg ctcggcggcc tgcacggcct
     1861 gcgccgcggt ggggaacacg gccatcagcc cgtcgccgag ggtcttgacc agcacgccac
     1921 ccgtggccgg cacccggcgg gcgatggcgt cgacggtctc cgtgacgacg gccgaggcgt
     1981 ccgcgttgcc gatcagctcg tacagcgcgg tgctgccgcg caggtccgcg aagacgacgg
     2041 cacgacgctg gatcgcggac atgcgttcag ctccgcgcgc cgccggtcgg cgcgacgaag
     2101 gggccccggc ggggcggctg tggcaggcgg cggcgcgtcg tcgtcacagg gctcagaggt
     2161 tgtccaggta ggtgcgcagc ttgtccgagc ggctcgggtg cttgagcttg cggatcgcct
     2221 tggcctcgat ctggcggatg cgctcgcgcg tgacgtcgaa ctgcttgccg acctcctcga
     2281 gcgtgtggtc ggtcgacatc tcgatgccga agcgcatgcg cagcaccttg gcctcgcgcg
     2341 gcgtgaggct gtcgaggatg tccttgacca cgtcgcgcag gccggcgtgc atcgcggcct
     2401 cgaccggggc gaggttgttg gtgtcctcga tgaagtcgcc caggtgcgag tcgtcgtcgt
     2461 cgccgatcgg cgtctccatg gagatcggct ccttggcgat cttcatgatc ttgcggatct
     2521 tgtcctcggg catctccatc ttctcggcga gcgtgggcgc gtccggctcg aagccgaatt
     2581 cctgcaggtg ctggcgcgag atgcggttca tcttgttgat cgtctcgatc atgtgcaccg
     2641 ggatgcggat cgtgcgggcc tggtcggcga tggagcgcgt gatcgcctgg cggatccacc
     2701 aggtcgcgta ggtcgagaac ttgtagccgc gacgatactc gaacttgtcc accgccttca
     2761 tcaggccgat gttgccttcc tggatcaggt cgaggaactg caggccgcgg ttggtgtact
     2821 tcttcgcgat ggagatcacc aggcgcaggt tggcctcgat catctccttc ttcgcgtcgc
     2881 gcgaggcctt ctcgccctcg ttcatcttgc ggttgatctc cttcaggtgc tcgatgggca
     2941 ccacggcgcg cgcctggagg tcgatcagct tctgctggag ttcctggatc gccggcaggt
     3001 tgcggctcat caccgcgctg tagcccttgt tgccggcgac ttccttctcg gcccagtgca
     3061 ggttgagcga gttcgtcggg aacgtcttga tgaagtgctc ctgcggcatg ccgcacttgt
     3121 cgacgacgat cttgcggatc tcgcgctcgt agcggcgcac gtcgtcgacc tggccgcgca
     3181 ggatggagca caggcgctcg atcgtcttga ccgtgaagcg gatcttcatc aggtcgccgg
     3241 tcagcgcttc ctgggccttg tcgtaggcgg gcgagcggta gccctccttc tcgaaggcct
     3301 tgcgcatctt gtcgaagtgc gtgctgagct cggcgaactt ggtcagcgcc tgggccttga
     3361 gctcctcgag cttcttggtc agggccttgg agccgccggc gccatcgtcg tcgtcctcct
     3421 cgtcgaactc gtcgaagtcc tcctcggcga cgtagtcgtc cgcctcgtcg tcggagacga
     3481 agccgtcgac cgcctcggag atctgcatct cgcccttctc gatgcgctcg gccagcgcga
     3541 ggatctccgc gatggtcgtg ggcgaggcgg agatcgcctg catcatggcc tgcaggccgc
     3601 cttcgatgcg cttggcgatc tcgatctcgc cctcgcgcgt gagcagctcg acggagccca
     3661 tctcgcgcat gtacatgcgc acggggtcgg tcgtgcggcc gaactcggaa tcgacggtcg
     3721 acagcgccgc ctcggcggcc tcctcggcct cttcctcggt cgcgccggtc tgcgcgctgc
     3781 cggagatcag cagcgtcgcg gcgtcgggcg cctgctcgta gaccgcgatg cccatgtcgt
     3841 tgagcatcga gacgatggcc tcgaggatct cgttgtcgac cagcttctcg ggcaggtggt
     3901 cgttgatctc ctggtgcgtc aggaaacccc gcgtcttgcc catcttgatg agggtcttga
     3961 gctcctggcg gcgcttggcg acttcctctt cggtgagctg cgtctcgtcc aggccgaact
     4021 cgcgcatgag cgcgcgctcc ttggcgcgcg agaccttcat gcgcagcggc ttggccttgg
     4081 cggccttgac ctcgccgacc tcctcgccac cctcgtcggc ggcctcgacc tcggcctcgg
     4141 cgtcaccctc gatgtcggcc tcgacgtcgg cgtcgtcctc gaagaccggg gccttgggct
     4201 cgtccttctt gcccttcttg gccgcggcgg ccgtcgtctt cgcggcaggc gccttcgcgg
     4261 ccttcgcggc ggcggtcttg gcggcgggcg ccggcttgtc ggccgccttc gtcgcggtcg
     4321 tcgccgtcgt gctccgggca gcggccttgg gcgcagcagc agcggacacc gccttcttct
     4381 cgaccggctt gtccgcggcc gcggtcttcg cggcgggcgt ggtcgtcttg gcagtatcgg
     4441 cgctcttctt ggcagtcatg cgtttccttg gatgaatgtc gtcgacccag gccgcccgga
     4501 ttcacgcgtc tcgcccacgt cccgacggcc tgccggcaga atgtgcggac aggcgcgacg
     4561 aaaaaaaggc gtgcgaacga tttcccgccg cgggtggcgc ggccggagct ccgagctggc
     4621 aagttggcgt gaacgggcga atatgcagcc cttgcgggtg gatttggccg cgtggaacgc
     4681 ccttgacgag ggtggccggg gcccggagtt ggctgctgtc actcttgccg ctgagtggga
     4741 tttcggatta tacctcggaa tcgtcctggg aagccagtcg cgacagccgt tccttgacct
     4801 ctttccagtg ctcgaacacg agctggtagc ggcgcaggtc gtcgggcgtg gagatcgacc
     4861 tcgacagctc gagcgagcgc gcgttgagca ggcgctccag cacctggtcg acggcacggc
     4921 ggaagtcgac atggcgtgcg tccggatgtg catcgccgtc ggcggccgcg cgcgtcgcct
     4981 cgtccagcga cgggtcgtcg gccagggcga ggcgaaggat ggcccagggc cgcgggccgt
     5041 gttcggccac gtcgcgctcc agccaggcca ccagcgggcc gtagggcgcg ggcagcgcgt
     5101 gcagcagctc atgctcggaa gccgacagct cgtcccagaa ctccgggtgc ccgaacagga
     5161 tctcgacgat gcgcgcggag ggcgtcttcg gggcggcgcg tggcaggcgc agcagttcgg
     5221 cgcgctcggc ctcctcgcgg tcgcgggctg cgaagcgtcc gccgaagcgg ccccgtccgc
     5281 cgccgcgcgg ctcccatggc cggtcgcgcc cgcgcgggc
//
LOCUS       DASVLN010000992         4161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_213558, whole genome shotgun sequence.
ACCESSION   DASVLN010000992 DASVLN010000000
VERSION     DASVLN010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213558"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..524
                     /locus_tag="VF453_23220"
     CDS             189..524
                     /locus_tag="VF453_23220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690634.1"
                     /translation="MSRSPVAPTPAPIAVERVQTGVRLEKRLLKVLKAIAELHDLSLG
                     DLLEGVVLHAFEGRCAFGPDTLGRIADLKRVYGLDLDAGAAHRLVEAAEISPPADAAP
                     RAPGSRRRS"
     gene            complement(469..1113)
                     /gene="kynB"
                     /locus_tag="VF453_23225"
     CDS             complement(469..1113)
                     /gene="kynB"
                     /locus_tag="VF453_23225"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HEX7690635.1"
                     /translation="MTKLWDISPPIDARSPVFPGDTTFRQEWGATIGPGCPVNVSAIT
                     LSPHTGAHADAPLHYEADAAAIGAVSLEPFLGPCRVIHAIGARPLVTMAHLAHALGRD
                     GRTLPPRVLVRTYARAPLDAFDPDCAAFEPATVEALADLGVTLVGIDTASVDPAASKT
                     LDSHQVLRRRGLRVLENLVLDEVPEGDYELIALPLKLTSADASPVRAVLRPLAG"
     gene            1174..1533
                     /locus_tag="VF453_23230"
     CDS             1174..1533
                     /locus_tag="VF453_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7690636.1"
                     /translation="MLEGSCHCGAVRLHVPSPPAELCLCNCSICRRYGALWAFYVAEE
                     TRVDGREHLASYVWGDRTIDTMHCRHCGCVTHWESLQPGPGGRRTFNMRLFDPAEIGE
                     PRLRRFDGADTWRYLDE"
     gene            complement(1573..3126)
                     /locus_tag="VF453_23235"
     CDS             complement(1573..3126)
                     /locus_tag="VF453_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HEX7690637.1"
                     /translation="MAEQKRPATIDSGGSLRSRFGRDVVDIGGLAPPLAKGAIVEGFL
                     LEKRLHQGGMASMWRVVRVDAEGQPAPNPDEPPLIMKVPRIKGGEDPATIVGYEVEQM
                     IMPALSGVHVPRYVARGDFTRQPYIVMEHIPGDTLRPRLAEAPLALDEVAEIGIKVAT
                     ALHDLHRQHVVHLDVKPSNIMFRPTGEAVLVDYGLSRHDHLPDLLEEEFSLPMGTGPY
                     MSPEQVQFIRNDPRSDLFALGVMLYHFTTGERPFGAPTSVRGLRKRLWIDPVPPRALR
                     SDCPPWLQEVILKCLEVHPDKRYQSAAQLALDLQQPDQIPLTRRAERMVRSSQWSRFK
                     RWFFSIGAEPPESAAQTSATEQINRSSIVMASVDIDNGTPELLEQLRETVRRIMLTEP
                     GARLACVSVMRIARIGTDALTDHQGKSLHVKQLVGLKHWARPISKALNLEDGRLTFHV
                     LEAPDPADALVDFARRNQVDHIVMGARGNSTLRRYLGSVSSTVVAESECTVTVVRAAT
                     PGEEDRSEQ"
     gene            complement(3128..3874)
                     /locus_tag="VF453_23240"
     CDS             complement(3128..3874)
                     /locus_tag="VF453_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7690638.1"
                     /translation="MTKIAVVTDLHANREAVEAVFAHARAQGATEWAFLGDYVGYGAD
                     PAWVIDQVREMVDAGAAAVCGNHDEAAVIGGTPQMRPEARLVIDWTRSQLDPGQLDFL
                     AALPFQLQRDDRLYVHANAYAPSQWEYVQGRADAVRSIHATTCHYTFCGHVHEPRLYH
                     LGATGKAGDFTPMPGVPIPLPGYRQWLVIPGSAGQPRDGNPAACYAMFEPQAQRITYH
                     RVPYDHETAAAKIRAAGLPERLAARLFEGV"
BASE COUNT          520 a         1467 c         1574 g          600 t
ORIGIN      
        1 cgtcgccgcg catccggagc tctccgaccg cgcgctcctg cggcgccact accgcgccgc
       61 gacactggcc gcgcccgagg cgcgcgagcg cttcgtgctg gagacgctcg cgccggaggt
      121 gcacgcatga cgacgtcccg gccgcccggc cgcgtcggcg ccgcaggcgt cgcgcgggag
      181 gtcgcgcgat gagccggtcc ccggtggcgc cgaccccggc cccgatcgcg gtcgagcggg
      241 tgcagaccgg cgtgcgcctc gagaagcgcc tgctcaaggt gctcaaggcg atcgcggagc
      301 tccacgacct gagcctgggc gacctgctcg agggcgtcgt cctgcatgcc ttcgagggtc
      361 gctgcgcgtt cgggcccgat acccttggcc gtatcgccga cctcaagcgc gtctacgggc
      421 tcgacctcga cgccggtgcc gcgcaccggc tcgtcgaggc ggccgagatc agcccgccag
      481 cggacgcagc accgcgcgca ccgggctcgc gtcggcgctc gtgagcttta gcggcagcgc
      541 gatgagctcg tagtcgccct cgggcacctc gtcgagcacg aggttctcga gcacgcgcag
      601 cccgcgccgg cgcagcacct ggtggctgtc cagcgtcttg ctcgcggcgg ggtcgacgct
      661 cgcggtgtcg atgcccacca gcgtcacgcc gaggtcggcc agcgcctcga ccgtcgcggg
      721 ctcgaacgcg gcgcaatcgg ggtcgaacgc gtccagcggc gcgcgcgcgt aggtgcgcac
      781 cagcacgcgc ggcggcagcg tgcggccgtc gcgcccgagt gcgtgcgcga ggtgcgccat
      841 ggtcacgagc ggcctcgcgc cgatcgcgtg gatcacgcga cacgggccga ggaagggctc
      901 gagcgagaca gcgccgatcg cggcggcgtc cgcctcgtag tgcaagggcg cgtcggcgtg
      961 cgcgccggtg tgcggcgaga gcgtgatggc gctgacgttg accgggcacc ccggcccgat
     1021 cgtcgcgccc cactcctgcc ggaaggtcgt gtcgcccggg aacacggggg agcgggcgtc
     1081 gattggcggc gagatgtccc agagcttggt catggccgtc gactttaaac ccgcgagctg
     1141 gtcgacgagg cgactgcccg gcatactccg ccgatgctcg aaggatcctg ccattgcggc
     1201 gccgtccggc tccatgtccc gtccccgccg gccgagctgt gcctgtgcaa ctgctcgatc
     1261 tgccgccgct acggcgcgct gtgggcgttc tacgtcgccg aggagacgcg cgtggacggc
     1321 cgcgagcatc tcgccagcta tgtctggggc gatcgcacga tcgacacgat gcactgccgg
     1381 cactgcggct gcgtcacgca ctgggagtcg ctgcaacccg gccccggcgg ccggcgcacc
     1441 ttcaacatgc ggctgttcga tcccgccgag atcggcgagc cgcgcctgcg tcgcttcgac
     1501 ggcgccgaca cctggagata cctcgacgag tgagcgccgg ccgggcgcgc cgggtcgccg
     1561 gcgggcgccg cctcactgtt cgctgcgatc ctcctcgccg ggagtcgcgg cgcgcacgac
     1621 ggtgaccgtg cactccgact ccgcgacgac ggtcgacgat acgctgccca ggtatcggcg
     1681 cagcgtcgag ttgccgcgcg cgcccatcac gatgtggtcg acctggttgc ggcgcgcgaa
     1741 gtcgacgagc gcgtcggccg ggtcgggggc ctcgagcacg tggaaggtca ggcggccgtc
     1801 ctcgaggttg agcgccttgc tgatcggccg cgcccagtgc ttcaggccga ccagctgctt
     1861 gacgtgcagg ctcttgccct ggtggtcggt gagcgcgtcg gtgccgatgc gggcgatgcg
     1921 catcacgctg acgcaggcca ggcgcgcgcc gggctccgtg agcatgatgc ggcgcacggt
     1981 ctcgcgcagc tgctcgagca gctcgggcgt gccgttgtcg atgtccaccg aggccatgac
     2041 gatggagctg cggttgatct gctcggtggc gctcgtctgg gccgcggact ccggcggctc
     2101 cgcgccgatg gagaagaacc agcgcttgaa gcgcgaccac tggctgctgc gaaccatgcg
     2161 ctcggctcgg cgcgtgagcg ggatctggtc gggctgctgg aggtcgagcg cgagctgcgc
     2221 ggcgctctgg tagcgcttgt ccggatgcac ctccaggcac ttgaggatca cctcctgcag
     2281 ccagggcggg cagtccgagc gcagcgcgcg tggcggcacc gggtcgatcc acaggcgctt
     2341 gcgcagcccg cgcaccgagg tcggcgcgcc gaacgggcgc tccccggtgg tgaagtggta
     2401 cagcatcacg ccgagcgcga acaggtcgct gcgcgggtcg ttgcggatga actgcacctg
     2461 ctcgggcgac atgtagggcc cggtgcccat gggcagcgag aactcctcct cgagcaggtc
     2521 gggcaggtgg tcgtggcgcg agaggccgta gtcgaccagc accgcctcgc ccgtgggccg
     2581 gaacatgatg ttgctgggct tcacgtccag gtgcacgacg tgctggcggt gcaggtcgtg
     2641 cagcgcggtg gcgaccttga tcccgatctc ggcgacctcg tcgagcgcga gcggcgcctc
     2701 ggccagccgc gggcgcagcg tgtcgcccgg gatgtgctcc atcacgatgt agggctggcg
     2761 cgtgaagtcg ccgcgggcga cgtagcgcgg cacgtgcacg ccggacagcg ccggcatgat
     2821 catctgctcg acctcgtagc cgacgatcgt ggcgggatcc tcgccgccct tgatgcgcgg
     2881 caccttcatg atcagcggcg gctcgtcggg gttcggtgcg ggctggccct cggcatcgac
     2941 gcgcaccacg cgccacatgc tggccatccc gccctggtgc aggcgcttct cgagcaggaa
     3001 gccctccacg atcgcgccct tggccagcgg cggggcgagg ccgccgatgt cgacgacgtc
     3061 gcggccgaag cgcgagcgca ggctgccgcc ggaatcgatc gtcgcgggtc gcttctgttc
     3121 ggccacgtca cactccctcg aagagccggg cggcgaggcg ctccggcagg ccggcggcgc
     3181 ggatcttggc ggcggcggtc tcgtgatcgt acggcacgcg gtggtaggtg atgcgctggg
     3241 cctggggctc gaacatggcg tagcaggcgg cggggttgcc gtcgcgcggc tggccggccg
     3301 aaccggggat gacgagccac tgccggtagc cgggcagcgg gatcggcacg ccgggcatgg
     3361 gcgtgaagtc gcccgccttg ccggtggcgc cgagatggta gagccgcggc tcgtggacgt
     3421 ggccgcagaa ggtgtagtgg caggtcgtcg cgtggatgct gcgcacggcg tcggcgcggc
     3481 cctggacgta ctcccactgg ctcggcgcgt aggcgttggc gtggacgtac aggcggtcgt
     3541 cgcgctgcag ctggaagggc agggcggcga ggaagtcgag ctggccggga tcgagctgcg
     3601 agcgcgtcca gtcgatgacg agccgcgcct ccggacgcat ctgcggcgtg ccgccgatga
     3661 cggctgcctc gtcgtggttg ccgcagacgg cggcggcgcc ggcgtcgacc atctcgcgta
     3721 cctggtcgat gacccacgcg gggtcggccc cgtagcccac gtagtcgccg aggaacgccc
     3781 actccgtggc gccctgggcg cgcgcgtgcg cgaacacggc ctcgacggcc tcccggttgg
     3841 cgtgcaggtc ggtcacgacc gcgatcttgg tcatcgtttc ttccagcccg gggacggatg
     3901 ttgccagcac tcgctgacgc gccgcgtacg ggattaccag ccgccgggcc ggtttgcggc
     3961 agactacgcc ccgcggccgc gccggcccgc cttgcccagg agcgaacccc atgcccgatg
     4021 cccagcgccg ccccgccgcg gtcgccgccg ctgccgtcct ggcgctggcg gcggctgccg
     4081 ccctcgtccc caccgcgcag gccgcgtccc gccgcgcgcc gccggcgcgc ggtgcgtggg
     4141 gagtcgtggt cgccgacggc c
//
LOCUS       DASVLN010000993         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_213568, whole genome shotgun sequence.
ACCESSION   DASVLN010000993 DASVLN010000000
VERSION     DASVLN010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213568"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..>1961)
                     /locus_tag="VF453_23245"
     CDS             complement(111..>1961)
                     /locus_tag="VF453_23245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7690639.1"
                     /translation="FGAETAAPPSAPASVPDIAQPPAPQVHNSILDGALLKQLLLADF
                     ALQDGQAGDAVEIYIQAGRRYKDDALFRRAYQIALQVGAGDKALAVTKAWRQAMPRSA
                     EATRTEVQVLVGLDRVAEAQEPLEQLLQMSPPAERGPLLASLPRVVERAKDRAAVAAE
                     ASELVKPYLDAPETSLPARVALARIDMLQGQAGEALRLAEQAHAQDPLATPPVMLALD
                     IMNALSGVAPTRAAGTAAPAGTEAPTKRDVPADAIPRSEAIVRDYLARKGADPVVRSF
                     YASVLSSEERLPEAAEQLRMATKERPKLAQLWIALGDAELELHHPDAAEAALKQALAL
                     AMADQAAHPSNGDEDDAAGDADAAAPGPRLDYIRLLLARAADQRRDDAAMEKWLGEID
                     TKDTDLQVIALRAEVLARRGKLAEALALVRAAPATSPAEERYRLLTQAQLLIESKKFA
                     DARELLAKANKESPDDVELIYQEAMVDERMNRLDDMETLLRRILVLQPQNSQALNALG
                     YSLADQNVRLDEALGLVKQAHALSPADPFIVDSLGWVEYRMGHYDTATTLLSQAYASR
                     KDTEIAAHLGEALWAEGRRDEAVQVLRDAHRLEPGNEVLRQTMARLKVTP"
BASE COUNT          225 a          712 c          737 g          287 t
ORIGIN      
        1 cagcggcggc ggatgcgtcg cgcaggcctg cagcgccagc gcgaacgcgg ccgcgagcag
       61 gcggccggcg gcgccgcggg cgagatccgg gagaaggcgg cgcacggacg tcagggcgtc
      121 accttgagcc gggccatcgt ctgccgcagc acctcgttgc ccggctccag ccggtgcgcg
      181 tcgcgcagca cctggacggc ctcgtcgcgc ctgccctcgg cccacagcgc ctcgcccagg
      241 tgggcggcga tctcggtgtc cttgcgcgag gcgtaggcct ggctcagcag cgtcgtcgcg
      301 gtgtcgtagt ggcccatccg gtactcgacc cagccgaggc tgtccacgat gaacgggtcc
      361 gccggcgaca gcgcatgcgc ctgcttcacg agcccgagcg cctcgtcgag ccgcacgttc
      421 tggtcggcga gcgaatagcc gagcgcgttc agcgcctgcg agttctgcgg ctgcagcacg
      481 aggatgcggc gcagcagcgt ctccatgtcg tcgaggcggt tcatgcgctc gtcgaccatc
      541 gcctcctggt agatcagctc gacgtcgtcg ggcgattcct tgttggcctt ggccagcagc
      601 tcgcgggcgt cggcgaactt cttgctctcg atcagcagct gcgcctgcgt cagcagccgg
      661 tagcgctcct cggcgggcga tgtcgccggc gcggcgcgca cgagggcgag cgcctcggcg
      721 agcttgccgc ggcgcgcgag cacctcggcg cgcagggcga tcacctgcag atcggtgtcc
      781 ttggtgtcga tctcgccgag ccacttctcc atcgccgcgt cgtcgcggcg ctggtcggcg
      841 gcgcgggcga gcagcaggcg gatgtagtcg aggcgcgggc cgggcgcggc ggcgtccgcg
      901 tcgccggcgg cgtcgtcctc gtcgccgttg gacgggtgcg ccgcctggtc ggccatcgcc
      961 agcgccaatg cctgcttcag cgcggcctcg gcggcgtcgg gatggtgcag ctcgagctcc
     1021 gcatcgccca gcgcgatcca cagctgcgcg agcttcgggc gctccttcgt ggccatgcgc
     1081 agctgctcgg cggcctcggg caggcgttcc tcgctggaca gcacgctcgc gtagaacgag
     1141 cgcacgaccg ggtcggcgcc cttcctcgcg aggtagtcgc gcacgatggc ctcggagcgc
     1201 gggatcgcgt cggcgggcac gtcccgcttc gtcggcgcct cggtgcccgc gggcgcagcg
     1261 gtgccggccg cgcgcgtggg cgccacgccc gacagcgcgt tcatgatgtc cagcgccagc
     1321 atcacgggcg gcgtggcgag cggatcctgc gcatgggcct gctcggcgag ccgcagcgcc
     1381 tcgccggcct ggccctgcag catgtcgatg cgggcgagcg cgacccgggc cggcaggctc
     1441 gtctcgggcg cgtcgaggta gggcttgacc agctcggatg cctccgcggc gaccgccgcg
     1501 cggtccttcg cgcgctcgac cacgcgaggc agactcgcca gcagcggccc gcgctcggca
     1561 ggcgggctca tctgcagcag ctgctcgagc ggctcctgcg cctcggcgac ccgatccagt
     1621 ccgaccagca cctgcacttc ggtgcgcgtg gcctcggcgg acctgggcat cgcctggcgc
     1681 caggccttgg tcaccgcgag ggccttgtcg cccgcgccga cctggagcgc gatctggtac
     1741 gcgcgccgga acagcgcgtc gtccttgtag cggcgcccgg cctggatgta gatttcgacc
     1801 gcgtcgccgg cctggccgtc ctgaagtgcg aagtcggcga gcagcaactg cttcaggagg
     1861 gcgccgtcca ggatcgagtt gtggacctgc ggcgccggcg gctgcgcgat gtcgggcacc
     1921 gaggcaggcg ccgacggcgg cgcggccgtc tccgcgccga a
//
LOCUS       DASVLN010000994         2540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_268038, whole genome shotgun sequence.
ACCESSION   DASVLN010000994 DASVLN010000000
VERSION     DASVLN010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268038"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VF453_23250"
     CDS             <1..595
                     /locus_tag="VF453_23250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690640.1"
                     /translation="DGGNERVIVARAGLAALLHEVEAASAAADVDDASFALANLFCVP
                     APASVPVGAMGDPLVGYDFVLAANYRRAIAALLADDRPMARHLGQPGPFLVAFRLPVA
                     EVRREPDAAPPPGSPNDVLMIDMSDVPAAAIPVYVSSFKSAVRREGSSGDTALAPLRA
                     RFAAALVDLNQALPAIEEAYAGTVKPLEAGAGEGDAH"
     gene            complement(605..1198)
                     /locus_tag="VF453_23255"
     CDS             complement(605..1198)
                     /locus_tag="VF453_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX7690641.1"
                     /translation="MPSRRAASDPPADDAPLRLAGPAAFDAWLARHHASSDGTWLQLA
                     KAGTPGLRYAEALEVALCHGWIDGQKKSLDAQHFLQRFTPRRARSLWSKINRDKALAL
                     IAAGRMRPAGLAQVEAAKADGRWDDAYDGARSAQVPPDLQAALDARPAARAFFATLDG
                     TNRYAVLWRVQTAKKPETRARRIEQLVGMLARGERIH"
     gene            complement(1214..1732)
                     /locus_tag="VF453_23260"
     CDS             complement(1214..1732)
                     /locus_tag="VF453_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX7690642.1"
                     /translation="MTDIHALASAPALPARRGALVVALLATLSCAVLVVAPACVHARP
                     AGAASAPVVDDSLYRALGGQPGIERLAATLVPRLAADERIGPFFKHARREHLQAMLAL
                     KFCELAGGGCDYTGRPLKEAHRDMDISRADFNALVEVLQSVMHDQGIPFATQNRLLAR
                     LAPLQRDVVNVR"
     gene            complement(1745..>2540)
                     /locus_tag="VF453_23265"
     CDS             complement(1745..>2540)
                     /locus_tag="VF453_23265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HEX7690643.1"
                     /translation="TSVDGAAGGGLTPWAVIGTNATEGEWGATAAVTKVATRNYTLDV
                     LAAAAAWDDRVELSVAHQDFDTGATGRALALPGLHLKLDIVGVKARVAGDAILDSDTL
                     LPQVAVGVELKSLDAGDLAPTLEALGARRRGTDAYVSATKLFLAQGVLVDATLRATRA
                     NQNGLLGFGGTAQDRYRLEPEFSIAWLASPRLAIGGEYRRKPDNLDPSLLGAGLKEDD
                     WVDLFVAWAPSRHLSITVAWVQLGRIVPAVATRRQDGAYVSAQSAF"
BASE COUNT          308 a          936 c          963 g          333 t
ORIGIN      
        1 cgacggcggc aacgagcgcg tgatcgtcgc ccgggccggc ctcgcggcgc tgctgcacga
       61 ggtcgaggcc gcgtccgccg ccgccgacgt cgacgacgcg agcttcgcgc tcgccaacct
      121 gttctgcgtc ccggcgccgg cctcggtgcc cgtcggcgcg atgggcgatc cgctggtcgg
      181 ctacgacttc gtcctcgccg cgaactatcg ccgcgcgatc gccgcgctgc tcgccgacga
      241 ccgcccgatg gcgcgccatc tgggccagcc cgggcccttc ctcgtcgcgt tccggctgcc
      301 cgtggccgag gtgcggcgcg agcccgacgc cgcgccgccg cccggctcgc ccaacgacgt
      361 gctgatgatc gacatgagcg acgtgcccgc cgccgcgatc ccggtctacg tgagcagctt
      421 caagtccgcc gtgcgccgcg agggcagctc cggcgacacc gcgctcgcgc cgctgcgcgc
      481 gcgcttcgca gcggcgctgg tcgatctgaa ccaggcgttg cccgcgatcg aggaggccta
      541 cgccggcacc gtcaagccgt tggaggccgg tgccggcgag ggcgacgccc actagcggca
      601 ggtcctagtg gatccgctcg ccgcgcgcga gcatgcccac cagctgctcg atgcgccgcg
      661 cgcgcgtctc cggcttcttc gcggtctgca cgcgccagag cacggcgtag cggttggtgc
      721 cgtccagcgt cgcgaagaag gcgcgcgccg ccggccgcgc gtcgagcgcc gcctgcaggt
      781 cgggcggcac ctgcgccgag cgcgcgccgt cgtatgcgtc gtcccagcgg ccgtcggcct
      841 tcgcggcctc gacctgcgcc aggccggccg gccgcatgcg gcccgcggcg atcagcgcga
      901 gcgccttgtc gcgattgatc ttcgaccaca ggctgcgcgc gcggcgcggc gtgaagcgct
      961 gcaggaaatg ctgcgcgtcg agcgacttct tctggccgtc gatccagccg tggcacagcg
     1021 cgacctccag cgcctcggca tagcgcagcc ccggcgtgcc ggccttggcg agctgcagcc
     1081 aggttccgtc cgagctcgcg tgatggcgcg cgagccaggc gtcgaaggcg gcgggacctg
     1141 ccaggcgcag cggagcgtcg tcggcgggcg ggtcggaagc ggcgcgtcgg gagggcatgc
     1201 gcccgagtgt gcctcagcgc acgttgacga cgtcgcgctg cagcggcgcc aggcgcgcga
     1261 gcaggcggtt ctgcgttgcg aacgggatgc cctggtcgtg catcaccgac tgcagcacct
     1321 cgacgagcgc gttgaagtcc gcgcgcgaga tgtccatgtc gcggtgggcc tccttcagcg
     1381 gtcggccggt gtagtcgcag ccgccgcccg cgagctcgca gaacttcagc gccagcatgg
     1441 cctgcaggtg ctccctgcgc gcgtgcttga agaacggccc gatgcgctcg tcggccgcca
     1501 ggcgcggcac cagcgtcgcg gcgaggcgct cgatgcccgg ctggccgccg agcgcgcgat
     1561 agaggctgtc gtcgacgacc ggcgcggacg cggcgcccgc cggtcgcgcg tgcacgcacg
     1621 cgggcgcgac gaccagcacg gcgcacgaca gcgtcgccag cagcgcgacg acgagcgcgc
     1681 cgcggcgtgc gggcagggca ggggcggagg cgagggcgtg gatgtcggtc atggcatgac
     1741 gggctcagaa cgccgattgc gcggacacgt aggcgccgtc ctggcgccgg gtggcgacgg
     1801 ccggcacgat gcggcccagc tgcacccacg cgacggtgat ggacaggtgc ctggacggcg
     1861 cccaggcgac gaacaggtcg acccagtcgt cctccttcag gcccgcgccg agcagcgagg
     1921 gatcgaggtt gtcgggcttg cgccggtact cgccgccgat cgcgagccgc gggctggcga
     1981 gccaggcgat cgagaactcc ggctcgaggc ggtagcgatc ctgcgcggtg ccgccgaagc
     2041 ccaggaggcc gttctggttg gccctggtcg cgcgcagcgt ggcgtcgacg agcacgccct
     2101 gcgcgaggaa cagcttggtc gcgctcacgt aggcgtcggt gccgcgccgg cgcgccccga
     2161 gcgcctcgag ggtgggcgcg aggtcgccgg cgtccagcga cttgagttcg acgccgaccg
     2221 ccacctgcgg cagcagggtg tcgctgtcga ggatggcgtc gcccgccacg cgcgccttga
     2281 cgccgacgat gtcgagcttc aggtgtagcc ccggcagggc cagggcgcgc ccggtcgcgc
     2341 cggtgtcgaa gtcctggtgc gcgaccgaga gctcgacccg gtcgtcccac gccgccgcgg
     2401 ccgccaggac atcgagcgtg tagttgcgcg tcgcgacctt cgtgaccgcg gcggtcgcgc
     2461 cccattcgcc ctcggtcgcg ttggtgccga tcacggccca gggcgtgagg ccgccgccgg
     2521 ccgcgccgtc gacgctcgtc
//
LOCUS       DASVLN010000995         9970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_429454, whole genome shotgun sequence.
ACCESSION   DASVLN010000995 DASVLN010000000
VERSION     DASVLN010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429454"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..838)
                     /locus_tag="VF453_23270"
     CDS             complement(41..838)
                     /locus_tag="VF453_23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7690644.1"
                     /translation="MSNPDQRVALVTGGARGIGLAIGQWFHARGHRVALLDIDRQTLD
                     RTVAAFDRPDEVIGVHADVRDAAQVRAGVAAIVAKFGRVDKLVNNAGIAVFKPVMETS
                     YAEFRDVLNTNLDGAFLCTQAVVPEMLKAGGGAIVNIASISGLRASTLRVAYGTSKAA
                     LLHMTKQLAVELGDAGIRVNAICPGPVETEMAKLVHSVAIRSDYHDAIPLGRYGTPEE
                     MASAVGFLCSDEASFVNGQFLAVDGGFDAAGVGLPTLRRRAAEAAGA"
     gene            complement(866..1273)
                     /locus_tag="VF453_23275"
     CDS             complement(866..1273)
                     /locus_tag="VF453_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HEX7690645.1"
                     /translation="MQHEPSIQPRVETGAMGRVAYARVAPNEDLVASIEKLCVAEGFR
                     HAFVRGALGSLVDACFAAPDGSARQVRGPAVEIVSLAGEVREQPDGSLRAAITGVVAD
                     TEGCIHGGPFVAGANPVCVTFEVTLEEWLPTPA"
     gene            complement(1263..1844)
                     /locus_tag="VF453_23280"
     CDS             complement(1263..1844)
                     /locus_tag="VF453_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HEX7690646.1"
                     /translation="MTASIRSAPLPRARTLMHPGPVGPVRLEHLHAATARHVRLALEP
                     GLSVREALIRPLHALGIRSASMTLLGGSLAQLLYCIAPADASGERVANYTRPIDAGAS
                     ALVFGNATLGIGPCGEPLVHCHATFVRADGLARGGHVVVDRSIVAAPIAVLATSLDGI
                     ALRIVHDAETNMPLMRPFPAQAAAGSGEIAHAT"
     gene            2041..2925
                     /locus_tag="VF453_23285"
     CDS             2041..2925
                     /locus_tag="VF453_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7690647.1"
                     /translation="MEMRHLRCLVAVAEELHFGRAARRLNLSQPPVSLAIKELEAELG
                     VTLFERNSRRIALTRQGEEALHDARAVLARAELLRRRAHESAQGRTGTLTLGFISLAP
                     YSFLPGALRRFMADYPKVKVTLQESTTDRILAELEIGGLDLGFIFASPGMSATLAYRP
                     LQREPLIVALPATHPLAKSARVPLERLAHERFLHFERHYGPMVFDAMVATCMRHGFSP
                     DLFPARQMHTIVSLVAGGIGVALVPASVKAMPREGVAYRPIQGEHTLVETGAAWRGDG
                     DDGSLAQAFLGTLPQPGR"
     gene            3146..4312
                     /locus_tag="VF453_23290"
     CDS             3146..4312
                     /locus_tag="VF453_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEX7690648.1"
                     /translation="MEQAHIVGWGHTPFGKLDALDLEQLIRDAALPAVEGAGLDLADI
                     DGIFVGHFNGGFVQQDFSGALVAVAMPELRHVPAVRVENACATGSAAIWAALDAVGSG
                     RVKHALVVGMEKMNTLPGPQIGETLLRCSYVREEADTPGGFAGVFGDIAGVYFERFGD
                     QSDALAAIAAKNHENGVRNPLAHMKRDLGFDFCRTPSEKNPFVAGPLKRSDCSLVSDG
                     AAALVIAREPVAGAVHRPVRWRSRTQVGEYLPLSRRDPTRFEGAALAWQRGLAAAGAT
                     LADLHFVETHDCFTIAELLEYEAMGLAPQGQGARVILEGVTRRDGRLPVNPSGGLKSR
                     GHPIGATGVSQHVMAAMQLTGTAGDMQLPRAGLGAVFNMGGAAVANYLSVLEAL"
     gene            4309..5928
                     /locus_tag="VF453_23295"
     CDS             4309..5928
                     /locus_tag="VF453_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX7690649.1"
                     /translation="MSPAQACNLSQLLRQTARLHPDRAGLIQGERQWTWREIDARVDA
                     AVAALRSLGVRRGQRILVLSRNSLPMFESGWVAFRLGAVWVPVNFRLTPPEVAVLAES
                     SGATTMLVDAGFEAHVEAARSASPALKTVIPLSGERAGELNWEALVAAHRGAEPCEAT
                     VQSDDPLWFFYTSGTTGRPKAGMLTHGQMAFIVTNHLADLIPGTTERDVSIAVAPLSH
                     GAGIHALLNVARGAATVLLPSEKMDPELVWSLVEKHRVTNLFTVPTIVKMLVEHPAVE
                     AHDHSSLRYVIYAGAPMYRADQQRALRVLGPVLVQYFGLGEVTGCITVLPPSMHSPDD
                     DSPDANVGSCGRPRTGMEVAILDEAMNPVPVGAIGEICCRGPGVFAGYHDNPEATAKA
                     LRGGWFHTGDLGRVDERGLLYITGRESDMYISGGSNVYPREVEEVLLTHPAVAEVAVL
                     GVPDPKWGEVGVAVVVARAGAGCTAEALLAHLEGRCARYRWPHRFVFWDALPRSGYGK
                     VVKKDLRERLFASGELWESTSPAKTRETSSA"
     gene            5998..7011
                     /locus_tag="VF453_23300"
     CDS             5998..7011
                     /locus_tag="VF453_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7690650.1"
                     /translation="MDFTRRQILAGAAAAGSLALPTTRAFAQKAEFTYKYANNLPVSH
                     PMNLRAKEMSDAIRAETKGRVDIQLYPNNQLGSDTDMLSQLRSGGIEFFTLSGLILST
                     LVPSSSISGVGFAFPNYDTVWKAMDGELGAWIRAQIAKKDLVAMDKIWDNGFRQTTSS
                     TKPIVTPEDFKGFKIRVPVSPLWTSMFKAFDAAPASINFAEVYSALQTKVVEGQENPL
                     AIISTAKLYEVQKYCSLTNHMWDGFWFLANKKAWEKLPPDLQAIVAKHINAAGVKERA
                     DVAELNASLQKDLTAKGMVFNATNADAFRAHLRKAGFYSEWKGKFGDEGWAILERSVG
                     KLS"
     gene            7131..8945
                     /locus_tag="VF453_23305"
     CDS             7131..8945
                     /locus_tag="VF453_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7690651.1"
                     /translation="MAKLETALGWLVEVPAAILVVADVAVLLAGVVARFVLHDPLTWS
                     DELASMLFVWLAMFGAVIALRRGEHMRMTAIVSRLGPDQRALMEAVATCACLAFLAMV
                     VWPAWQHARDEGDITTPALEISNLWREASMPAGIVLMGVFAVLRLLRDATWRHALAGA
                     AIVAALCGAFWAGHGMFADLGRLNLLVFFVGVAAGSVLAGIPIAFAFGLGTFGYLALG
                     TDTPLTVVVGRMDEGMSHIILLAVPLFVFLGQLIEATGMARAMVAFLAGLLGHVRGGL
                     SYVLVGAMYLVSGISGAKAADMAAIAPALFPEMKKRGADEGELVALLAATGAQTETVP
                     PSLVLITIGSVTGVSIAALFTGGLLPALVLGLVLCTVVGVRARGEPLAAAPRTSAKTK
                     LKLFAVAFPALALPFLIRAAVVEGVATATEVSTIGIAYTLVVGALVYRELEWRRLGPM
                     LVGTAALSGAILLIIGTATAMAWGLTQSGFSGWLASTMSTLPGGAPMFLAVSVLAFVI
                     LGSVLEGIPAMVLFGPLLFPIARQLGIHEVHYAMVATLSMGLGLFAPPLGVGYYAACA
                     IARVNPDRGIRPMARYMLALLAGTVLVAAVPWLSTGFL"
     gene            complement(8982..9395)
                     /locus_tag="VF453_23310"
     CDS             complement(8982..9395)
                     /locus_tag="VF453_23310"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7690652.1"
                     /translation="MTETASRTAACACGQLRITLAGALRGVGVCHCLECQRRTGTAFA
                     TLASFDGPWSVCGVATEYVRAGDQGARFRFRFCPRCGSTVFHTEEGVEGGVGVAVGCF
                     ADPHFEAPQVSVYDCRRHPWVRLPDGVEAFDRDPS"
     gene            complement(9486..>9970)
                     /locus_tag="VF453_23315"
     CDS             complement(9486..>9970)
                     /locus_tag="VF453_23315"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX7690653.1"
                     /translation="VYLLARAERPAGAWPAGPLQAYRDGTLVGRVDWQPARGQKLEVA
                     LGQDDRVRIDAETPGDVTGSAGIFGGRGQKRVATVYAIANQHGTPVTVEVLDAAPVAR
                     DEAIRVETRFDPPPATTEWDHRAGVAAWTLSLAPQQTQRIRVEHVVSWPKERQVSALP
                     "
BASE COUNT         1308 a         3586 c         3629 g         1447 t
ORIGIN      
        1 gaaggcccag gccgccgatt cgtgaagcac ggtctgcatg tcaggctccg gcggcctcgg
       61 cggctcggcg ccgcagcgtc ggcaggccca cgccggccgc gtcgaagccg ccgtccaccg
      121 ccaggaactg gccgttgacg aagctcgcct cgtcgctgca caggaagccg acggcgctgg
      181 ccatctcctc gggcgtgccg tagcggccca gcgggatcgc gtcgtggtag tcggagcgga
      241 tcgccacgct gtgcacgagc ttggccatct cggtctccac cggccccggg cagatcgcgt
      301 tgacgcggat gccggcgtcg cccagctcca ccgccagctg cttggtcatg tgcagcagcg
      361 ccgccttgct cgtgccgtag gcgacgcgca gggtgctcgc gcgcaggccc gagatcgacg
      421 cgatgttgac gatcgcgccg ccgccggcct tgagcatctc cggcaccacc gcctgcgtgc
      481 agaggaaggc gccgtcgagg ttggtgttca ggacgtcgcg gaactcggcg tagctggtct
      541 ccatcaccgg cttgaagacg gcgatgccgg cgttgttgac gagcttgtcg acgcgcccga
      601 acttggcgac gatcgccgcg acgccggcgc gcacctgcgc cgcgtcgcgc acgtccgcgt
      661 ggacgccgat cacttcgtcg ggccggtcga aggcggcgac cgtgcggtct agggtctgcc
      721 tgtcgatgtc cagcagcgcg acgcggtggc cgcgggcgtg gaaccactgg ccgatcgcca
      781 ggccgatgcc gcgggcgccg ccggtgacga gggcgacgcg ctggtcgggg ttgctcatgt
      841 cggatgtctc ctgtgggaat gctgttcagg cgggggtggg cagccactcc tcgagggtga
      901 cctcgaaggt gacgcagacg gggttggctc cggcgacgaa cgggccgccg tggatgcagc
      961 cctcggtgtc ggcgacgacg ccggtgatgg ccgcgcgcag cgagccgtcg ggctgctcgc
     1021 gcacctcgcc ggccaggctc acgatctcga ccgccggccc gcgcacctgg cgtgcggagc
     1081 cgtcgggcgc ggcgaagcag gcgtcgacca ggctgccgag cgcgccgcgc acgaacgcgt
     1141 ggcggaagcc ctcggcgacg cacagcttct cgatgctggc gacgaggtcc tcgttgggcg
     1201 cgacgcgcgc gtaggcgacc cggcccatgg cgccggtctc gacgcggggt tggatggacg
     1261 gctcatgttg catgggcgat ctccccggat cccgccgcgg cctgcgccgg gaagggccgc
     1321 atcagcggca tgttggtctc ggcgtcgtgc acgatgcgca gcgcgatgcc gtccagcgag
     1381 gtcgcgagca ccgcgatcgg cgccgccacg atgctgcggt cgacgaccac gtggccgccg
     1441 cgcgcgaggc cgtccgcgcg cacgaaggtc gcgtggcagt gcacgagcgg ctcgccgcac
     1501 ggcccgatgc ccagcgtcgc gttgccgaag acgagcgccg aggcgccggc gtcgatcggc
     1561 cgcgtgtagt tggcgacgcg ctcgccgctc gcgtcggccg gcgcgatgca gtagagcaac
     1621 tgcgcgagcg agccgcccag cagcgtcatc gacgccgagc ggatgcccag cgcgtgcagg
     1681 gggcggatga gggcctcgcg caccgacagg ccgggctcca gcgcgagccg cacgtggcgc
     1741 gcggtcgcgg cgtgcaggtg ctcgaggcgg acgggcccca ccgggccggg gtgcatcaag
     1801 gtgcgggcgc gcggcagggg cgcgctgcgg atcgaggctg tcaaggcgtc tccttcgatg
     1861 atctggagtc tgcgtcgtgc ggactccgac atgagcgcct tcggagcggc cgggtggcgc
     1921 tcatggcgct ttgccgccgg actcgccggg tagggcggtt cggcatgcga tccagtctag
     1981 gaaaaggctt tcattcgggg aagtttgcaa ttcggatcga tccatccaag aatcggatcg
     2041 atggaaatgc gtcacctgcg ctgcctcgtc gccgtcgccg aggagctgca cttcggccgc
     2101 gccgcccggc ggctcaacct ctcgcagccg ccggtgagcc tggcgatcaa ggagctcgag
     2161 gccgagctgg gcgtcacgct gttcgagcgc aactcgcggc gcatcgcgct cacgcgccag
     2221 ggcgaggagg cgctgcacga cgcccgcgcc gtgctcgcgc gcgccgagct gctgcgccgg
     2281 cgcgcgcacg agtcggcgca agggcggacg ggcacgctga cgctgggctt catcagcctc
     2341 gcgccgtact cgttcctgcc cggcgcgctg cggcgcttca tggccgacta cccgaaggtc
     2401 aaggtcacgc tgcaggagtc gaccaccgac cgcatcctcg ccgagctcga gatcggcggg
     2461 ctggacctcg gcttcatctt cgcgtcgccc ggcatgtcgg cgacgctcgc ctaccggccg
     2521 ctgcagcgcg agccgctgat cgtcgcgctg ccggcgacgc atccgctcgc gaagtcggcg
     2581 cgggtgccgc tcgagcggct ggcgcacgag cgcttcctgc acttcgagcg ccactacggc
     2641 ccgatggtct tcgacgcgat ggtcgccacc tgcatgcgcc acggcttcag ccccgacctg
     2701 ttcccggcgc gccagatgca caccatcgtg agcctggtgg ccggcggcat cggcgtcgcg
     2761 ctggtgccgg cctcggtcaa ggcgatgccg cgcgagggcg tggcctaccg tccgatccag
     2821 ggcgagcaca cgctcgtcga gaccggcgcc gcctggcgcg gcgacggcga cgacggctcg
     2881 ctcgcgcagg ccttcctcgg cacgctgccg cagccggggc gctgacacgt tcgcatgtcg
     2941 gaccgtcggc cctgggcacg cggcgccggg gtcgatcggc cggcttccgg ccagcatgtg
     3001 aaccggtttc tgcgatgcgg cgccgcagca tgcgcgccgc gacgcggggc gcatgtccag
     3061 aatcgctgca cacgcaccgg acgcgggcct tgcagcggtt cacatcccga accccggccc
     3121 gtcgtcccga caggagacca cccgcatgga acaagcgcat atcgtcggct ggggccacac
     3181 gccgttcggc aagctcgacg ccctcgacct cgaacaactc atccgcgacg ccgcgctgcc
     3241 ggccgtcgag ggcgccggcc tggacctggc cgacatcgac ggcatcttcg tcggccactt
     3301 caacggcggc ttcgtccagc aggacttcag cggcgcgctg gtggccgtgg cgatgcccga
     3361 gctgcgccac gtgcccgcgg tgcgggtcga gaacgcctgc gccaccggct cggcggcgat
     3421 ctgggcggcg ctggacgcgg tcggctcggg ccgcgtgaag cacgcgctcg tggtcggcat
     3481 ggagaagatg aacacgctgc ccggaccgca gatcggcgag acgctgctgc gctgctccta
     3541 cgtgcgcgag gaggccgaca cccccggggg attcgcgggc gtcttcggcg acatcgcggg
     3601 cgtctacttc gagcgcttcg gcgaccagtc cgacgcgctc gccgccatcg ccgcgaagaa
     3661 ccacgagaac ggcgtgcgca atccgctcgc gcacatgaag cgcgacctgg gcttcgactt
     3721 ctgccgcacg ccgtccgaga agaacccgtt cgtcgccggg ccgctgaagc gctccgactg
     3781 ctccctcgtg tccgacggcg cggccgcgct cgtcatcgcg cgcgagccgg tggccggcgc
     3841 ggtgcatcga ccggtgcgct ggcgctcgcg cacgcaggtg ggcgagtacc tgccgctgtc
     3901 gcgccgcgac ccgacgcgct tcgagggcgc ggcgctcgcg tggcagcgcg gcctggcggc
     3961 cgcgggcgcc acgctggccg acctgcactt cgtcgagacc cacgactgct tcaccatcgc
     4021 cgagctgctg gagtacgagg cgatgggcct cgcgccgcag gggcagggcg cgcgcgtgat
     4081 cctcgagggc gtgacgaggc gcgacggccg gctgcccgtg aacccgtccg gcggcctgaa
     4141 gtcgcgcggc catccgatcg gcgcgaccgg cgtctcccag cacgtgatgg ccgcgatgca
     4201 gctgaccggc acggccggcg acatgcagct gccgcgcgcc ggcctcggcg ccgtcttcaa
     4261 catgggcggc gcggcggtgg cgaactacct gtcggtgctg gaggcgctgt gagcccggcc
     4321 caggcctgca acctctcgca gctgctgcgc cagaccgcgc gcctgcatcc cgaccgcgcc
     4381 ggcctgatcc agggcgagcg gcagtggacg tggcgcgaga tcgacgcccg cgtcgacgcc
     4441 gcggtcgcgg cgctgcggtc gctgggcgtg cggcgcgggc agcgcatcct cgtgctctcg
     4501 cgcaactcgc tgccgatgtt cgagagcggc tgggtcgcgt tccggctcgg cgcggtgtgg
     4561 gtgccggtga acttccgcct cacgccgccc gaggtcgccg tgctcgccga gtccagcggc
     4621 gcgacgacga tgctggtcga cgccggcttc gaggcgcacg tcgaggccgc gcggtccgcg
     4681 tcgcccgcgc tgaagacggt gattccgctg tccggcgaac gcgcgggcga gctgaactgg
     4741 gaggcgctgg tcgcggccca tcgcggcgcc gagccctgcg aagcgacggt gcagtccgac
     4801 gacccgctct ggttcttcta cacctcgggc accaccggcc ggcccaaggc gggcatgctg
     4861 acgcacggcc agatggcctt catcgtcacc aaccacctgg ccgacctgat ccccggcacc
     4921 accgagcgcg acgtctccat cgcggtggcg ccgctgtcgc acggggcggg catccacgcg
     4981 ctgctcaacg tcgcccgcgg cgcggccacc gtgctgctgc cgtccgagaa gatggatccc
     5041 gagctcgtgt ggtcgctcgt cgagaagcac cgcgtgacca acctgttcac ggtgcccacc
     5101 atcgtgaaga tgctcgtcga gcatcccgcc gtggaggcgc acgaccactc gtcgctgcgc
     5161 tacgtgatct acgccggcgc gccgatgtac cgggccgacc agcagcgcgc gctgcgcgtg
     5221 ctcgggccgg tgctggtgca gtacttcggg ctgggcgagg tgaccggctg catcaccgtg
     5281 ctgccgccgt ccatgcattc gcccgacgac gactcgcccg acgccaacgt cgggtcgtgc
     5341 ggccggccgc gcacgggcat ggaggtcgcg atcctggacg aggccatgaa cccggtgccg
     5401 gtcggcgcga tcggcgagat ctgctgccgc ggcccgggcg tcttcgcggg gtaccacgac
     5461 aaccccgagg ccacggccaa ggcgctgcgc ggcggctggt tccacaccgg cgacctcggc
     5521 cgcgtcgacg agcgcgggct gctctacatc acgggccgcg agtcggacat gtacatctcc
     5581 ggcggctcca acgtctaccc gcgcgaggtc gaggaggtgc tgctcacgca tccggccgtc
     5641 gccgaggtcg cggtgctggg cgtgcccgat ccgaagtggg gcgaggtcgg cgtggccgtg
     5701 gtcgtcgcac gcgcgggcgc gggctgcacg gccgaggcgc tgctcgcgca cctcgagggc
     5761 cgctgcgcgc gttaccgctg gccgcatcgc ttcgtcttct gggacgcgct gccgcgctcg
     5821 ggctacggca aggtcgtcaa gaaggacctg cgcgaacgac tcttcgcgag tggtgaattg
     5881 tgggaatcca catcccccgc gaagacccgg gagacgagct cggcctgaac ccacagaatc
     5941 ggccttcggc cgaacacccc ggcattcctc gccatcgact acaacggaga caagcacatg
     6001 gatttcaccc gtcgccagat cctcgccggt gccgccgccg cgggctcgct cgccttgccg
     6061 acgacccgcg ccttcgcgca gaaggcggag ttcacgtaca agtacgcgaa caacctgccc
     6121 gtctcgcacc cgatgaacct gcgcgccaag gagatgtccg acgcgatccg ggccgagacc
     6181 aagggccgcg tcgacatcca gctctacccg aacaaccagc tcggctccga caccgacatg
     6241 ctgagccagc tgcgctccgg cggcatcgag ttcttcacgc tgtcgggcct gatcctgtcg
     6301 acgctggtgc ccagctcgtc gatcagcggc gtcggcttcg cgttcccgaa ctacgacacc
     6361 gtctggaagg cgatggacgg cgagctcggc gcctggatcc gcgcccagat cgcgaagaag
     6421 gacctcgtcg cgatggacaa gatctgggac aacggcttcc gccagaccac gtcctcgacc
     6481 aagcccatcg tcacgccgga ggacttcaag ggcttcaaga tccgcgtgcc cgtctcgccg
     6541 ctgtggacgt cgatgttcaa ggccttcgac gccgcacccg cctcgatcaa cttcgccgag
     6601 gtctactccg cgctgcagac caaggtcgtc gagggccagg agaacccgct ggccatcatc
     6661 tcgacggcca agctctacga ggtgcagaag tactgctcgc tgaccaacca catgtgggac
     6721 ggcttctggt tcctcgccaa caagaaggcg tgggagaagc tgccgcccga cctgcaggcc
     6781 atcgtcgcca agcacatcaa cgcggccggc gtgaaggagc gtgccgacgt cgccgagctc
     6841 aacgccagcc tgcagaagga tctgaccgcc aagggcatgg tcttcaacgc caccaacgcc
     6901 gacgccttcc gcgcccacct gcgcaaggcg ggcttctaca gcgagtggaa gggcaagttc
     6961 ggcgacgaag gctgggccat cctcgaacgc agcgtcggca agctgtcgtg aagcgcgccg
     7021 tccgactccg cgagctggcg gcggcgcccg gcgccgatcc gctggagggc gtgctgcccg
     7081 aagccgccaa cggcccgctc accgccacca cgcgcgcgcc gcgcggtgcg ctggcgaagc
     7141 tggagacggc gctgggctgg ctggtcgagg tgccggcggc catcctggtg gtggccgacg
     7201 tcgccgtgct gctggcgggc gtcgtcgcgc gcttcgtgct gcacgacccg ctcacgtggt
     7261 cggacgagct cgcctcgatg ctgttcgtct ggctcgcgat gttcggcgcc gtgatcgcgc
     7321 tgcgccgcgg cgagcacatg cgcatgacgg cgatcgtgtc ccggctcggg cccgaccagc
     7381 gcgcgctgat ggaggcggtc gcgacctgcg cctgcctcgc cttcctcgcg atggtggtct
     7441 ggccggcctg gcagcatgcg cgcgacgaag gcgatatcac gacgccggcg ctggagatct
     7501 ccaacctctg gcgcgaggcc tcgatgccgg cgggcatcgt gctgatgggc gtgttcgccg
     7561 tcctgcgcct gctgcgcgac gccacctggc gccacgcgct cgcgggcgcg gcgatcgtgg
     7621 cggcgctgtg cggcgcgttc tgggccgggc acggcatgtt cgccgacctg gggcggctga
     7681 acctgctcgt cttcttcgtc ggtgtcgcgg cgggcagcgt gctcgcgggc atcccgatcg
     7741 cgttcgcgtt cgggctgggc accttcggct acctcgcgct ggggaccgac acgccgctga
     7801 cggtcgtcgt cggccgcatg gacgagggca tgtcgcacat catcctgctg gcggtgcccc
     7861 tgttcgtgtt cctcggccag ctgatcgagg cgaccggcat ggcgcgcgcg atggtggcct
     7921 tcctcgccgg cctgctgggc catgtgcgcg gcggcctgtc gtacgtgctg gtgggcgcga
     7981 tgtacctggt ctccggcatc tcgggcgcca aggccgccga catggccgcc atcgcgcccg
     8041 cgctgttccc agagatgaag aaacgcggcg ccgacgaggg cgagctggtc gcgctgctgg
     8101 cggccaccgg cgcgcagacg gagaccgtgc cgcccagcct ggtgctgatc acgatcggct
     8161 cggtcaccgg cgtgtcgatc gcggccctgt tcaccggcgg gctgcttccg gcgctggtcc
     8221 tgggcctggt gctgtgcacg gtggtcggcg tgcgcgcgcg cggcgagccc ctggccgcgg
     8281 cgccgcgcac gtccgcgaag accaagctca agctgttcgc ggtcgcgttc ccggcgctcg
     8341 cgctgccctt cctcatccgc gcggcggtgg tcgagggcgt ggccacggcc accgaggtct
     8401 cgaccatcgg catcgcgtac acgctggtcg tcggcgcgct ggtctatcgc gagctcgaat
     8461 ggcgccggct cggcccgatg ctcgtgggca cggcggcgct gtcgggggcg atcctgctga
     8521 tcatcggcac cgccacggcg atggcctggg gcctgacgca gtcgggcttc tccggctggc
     8581 tcgcgagcac gatgtcgacc ctgcccggcg gcgcgccgat gttcctcgcg gtgtcggtgc
     8641 tcgcgttcgt gatcctcggc tcggtgctcg agggcatccc ggcgatggtg ctgttcgggc
     8701 cgctgctgtt cccgatcgcg cgccagctgg gtatccacga ggtgcactac gcgatggtcg
     8761 ccacgctctc gatgggcctg ggcctgttcg cgccgccgct gggcgtcggc tactacgcgg
     8821 cctgcgcgat cgcgcgtgtc aaccccgacc gcggcatccg gccgatggcg cgctacatgt
     8881 tggcgctgct ggccggcacg gtgctggtcg ccgcggtgcc gtggctgtcg acgggtttcc
     8941 tctgagcccg cccatgtcgc cccgaccggc cgcgcggcgg gtcaggacgg atccctgtcg
     9001 aacgcctcga cgccgtcggg cagccgcacc cagggatgtc gccggcagtc gtagaccgac
     9061 acctgaggcg cctcgaagtg gggatcggcg aagcagccca ccgccacgcc cacgccgccc
     9121 tcgacgcctt cctcggtgtg gaagacggtg ctgccgcagc gcgggcagaa gcggaagcgg
     9181 aagcgcgcgc cctggtcgcc ggcgcgcacg tactccgtcg cgacgccgca gaccgaccac
     9241 gggccgtcga acgaggccag cgtcgcgaag gcggtgcccg tgcggcgctg gcattccagg
     9301 cagtggcaca cgccgacgcc gcgcagcgcg ccggccagcg tgatgcgcag ctgaccgcac
     9361 gcgcaggcgg cggtacggga ggcagtctcg gtcatggcgg gctccggcgc ggcgacgagg
     9421 cgcaggatag ccgaccgccc cgggcgcggg acgagccgtc gcgggcggcg cctcggagga
     9481 cctgttcagg gcagggcgct gacctgccgc tccttcggcc agctgaccac gtgctcgacg
     9541 cggatgcgct gcgtctgctg cggcgcgagc gagagcgtcc acgcggcgac gccggcgcgg
     9601 tgatcccatt cggtggtggc cggcggcggg tcgaaccggg tctcgacgcg gatcgcctcg
     9661 tcgcgcgcga ccggcgcggc gtcgagcacc tccaccgtca ccggcgtgcc gtgctggttg
     9721 gcgatcgcgt agaccgtcgc gacacgcttc tggccgcggc cgccgaagat gccggcgctg
     9781 ccggtgacgt cgccgggcgt ctcggcgtcg atgcgcacgc ggtcgtcctg gcccagcgcg
     9841 acctcgagct tctggccgcg tgcgggctgc cagtcgacgc ggcccaccag cgtgccgtcg
     9901 cgataggcct gcagcgggcc cgcgggccat gcgccggcgg ggcgctccgc gcgcgcgagc
     9961 aggtagacgg
//
LOCUS       DASVLN010000996        10001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_429523, whole genome shotgun sequence.
ACCESSION   DASVLN010000996 DASVLN010000000
VERSION     DASVLN010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10001
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429523"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1390)
                     /locus_tag="VF453_23320"
     CDS             complement(<1..1390)
                     /locus_tag="VF453_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7690654.1"
                     /translation="MNFDDASSHGATHEVFNQVPPWQDVNLFERNAALRDAVAFNRPG
                     VDTTALAALGAEAGSARMQAHARLANLHGPSLHTHDRTGRRIDEVEFHPSYHVLLGAA
                     LRHRLHGAPWAAGGPSHVERAAGFMLFTELEPSVLCPVSMTYAVTPALRANSALQQAF
                     GGLLASPEYDERFIDPTAKTAVTMGMGMTEKQGGSDVRANTTRAVRDGDDAWGERWRL
                     VGHKWFMSAPMSDAFLVVAQAPAGPTCFFLPRRLPDGSPNGALNAVRIQRLKDKLGNK
                     ANASSEVEFVDAFAWRVGDEGRGLPQILAMGALTRLDCALGTAGLMRGALSIALHHAA
                     NRHAFSRRLDEHALMRNVLADLALESEAATALALRVARAVDASEDAAAPAAAREHESL
                     MRRLLTPAAKFWVCKRGSHFAQEAMECLGGNGYVEGECEGVMARIYREMPLNSIWEGA
                     GNIMALDLLRAQR"
     gene            complement(1450..1908)
                     /locus_tag="VF453_23325"
     CDS             complement(1450..1908)
                     /locus_tag="VF453_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7690655.1"
                     /translation="MSISWNWTRFEGLSADDVYDALALRAAIFVVEQRCAYLDVDGLD
                     RHAWHLLGRGEDGRLLAYVRVVDPGRKYEVPSVGRVVVDASQRGTGLGHELIAEALRR
                     CDATWPGAANRIGAQAHLQAFYGRHGYVTVGEAYLEDDIPHVDMERAGRS"
     gene            complement(1908..2927)
                     /locus_tag="VF453_23330"
     CDS             complement(1908..2927)
                     /locus_tag="VF453_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX7690656.1"
                     /translation="MTIVNRQIQLASRPEGEPAASNFRLVEQPLGPLADGQVLVRHEW
                     LSLDPYMRGRMNDAKSYAAPQPLDQVMIGGTAGEVVESRNPHFQVGDKVVGMGGWQEY
                     AVVDAGQRGVLQKVDTTHVPLSAYLGAVGMPGVTGWYGLVKIIAPKAGETVVVSSAAG
                     AVGGAVGQLAKARGCRVVGIAGGAEKCRHVVEDLGFDACIDYRQHTDLKAMSGALKAA
                     CPQGIDGYFENVGGLLLDAVMLRANAFARVAMCGMIAGYNGEPIPMTLPQLILTNRMR
                     IEGFIVSEHMEVWPEALKELGTLVATGRLKYRESIAHGLENAPEAFFGLLKGRNQGKQ
                     LVKLA"
     gene            complement(2982..3410)
                     /locus_tag="VF453_23335"
     CDS             complement(2982..3410)
                     /locus_tag="VF453_23335"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7690657.1"
                     /translation="MTDDDRQPIPFAVDIPFVEALGMRLWRFDGGHAEVSLDLRPDML
                     NSWQVAHGGVLMTLLDVAMAHAARSVRDPSEHGSPGIVTVEMKTSFMRPAHGRLVAFG
                     TLLHGGASTAFCEGSVRDETGALCAHATGTFKYLRTLRRP"
     gene            complement(3412..4242)
                     /locus_tag="VF453_23340"
     CDS             complement(3412..4242)
                     /locus_tag="VF453_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7690658.1"
                     /translation="MTSSAIPAVPRDERRRVQQLFDLSGRTALVTGGSRGLGLQIAEA
                     LGEAGARVLLSARKAPELETAVAALCERGAEADWIAADASQPAEVERLAAAARERLGE
                     VDVLVNNAGATWGAPAEEHPLEAWDKVMNLNVRAIFLLTQAVGRASMIPRRRGRVINV
                     ASIAGLSGNAAGAMKTVAYNTSKAAVINFTRALAGEWGAYGIQVNALAPGFFPSRMTA
                     GLLQAVGEDAMAAHAPLRRIGDDEDLKGAALLLASDAGKHITGQVIAVDGGVSAVHGG
                     "
     gene            complement(4268..6076)
                     /locus_tag="VF453_23345"
     CDS             complement(4268..6076)
                     /locus_tag="VF453_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7690659.1"
                     /translation="MTTRETLDFLLHDWLRVDALCARPRFAEHSRATFDAALDLCERL
                     AREKFAPFNRLVDTEEPAFDGERVTLPRATHDALRAYAASGMLAASQDAEVGGMQLPC
                     VVEKAGNAFFSQACIALGAYAMLTTGNARLLMAHGTPRQREVFAAREFAGEWFGTMCL
                     SEPQAGSSLGDIATRAVPDGAGFDADPLGPRYRLAGNKMWISAGEHEITANIVHLVLA
                     KIPGADGKPVPGTRGISLFIVPKWLVDADGRLTGERNDVRLAGLNHKLGYRGTTNALL
                     NFGEQGGAIGYRVGAPGDGLRCMFHMMNEARIGVGLGATMLGAAGYDASLAYARERTQ
                     GRCVGAGGKDAAAPPVRLIEHADVRRMLLAQKAIVEGGMALALYCARLVDEEHTGDAQ
                     AAAQAALLLEMLTPIAKSWPSEWALEANSLAIQVLGGYGYTRDFPVEQHWRDNRLNMI
                     HEGTHGIQALDLLGRKAVMQGGAGLKLLAHRIDATVARALHHERLAMHANALARALQK
                     LGAATRAAWATSAPEEALANATPYLQAFGHVVLAWLWLDVAAAVVDRGAGDAASRGRL
                     AALDYFMHFELPKVDAWLRVVETRDRTCLDMPEDAF"
     gene            complement(6210..7385)
                     /locus_tag="VF453_23350"
     CDS             complement(6210..7385)
                     /locus_tag="VF453_23350"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7690660.1"
                     /translation="MTEAVIVSTARTPLAKSWKGAFNMTHGAELGGHAVRAAIERAGI
                     DGAEVEDVLMGCANPEGATGANIARQVALRAGLPVSVAGVTVNRFCSSGLQTIAMAAQ
                     RVIAGEGDVYVAGGVESISCVQQEMNQHMLRDMWLAKNKPEIYWSMLETAENVAKRYK
                     ISKERQDRYGAESQQKACAAQAAGRFAEEIAPITVTAGVADPKNGIRTRQVTVAVDEG
                     LREGTTYEGIKDIRTAVPGGVITAGNASQFSDGGGACVVVSSQYAERKGLKPLGRFLG
                     FAVAGCEPDEMGIGPVYAVPKVLKRLGLTVGDIDLWELNEAFAVQVLYCADTLGIPMD
                     RLNVNGGAIAVGHPYGVSGQRLTGHALVEGRRRGAKKVCVTMCIGGGMGAAGVFEVL"
     gene            complement(7455..7661)
                     /locus_tag="VF453_23355"
     CDS             complement(7455..7661)
                     /locus_tag="VF453_23355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690661.1"
                     /translation="MVAGLLKRAMLVTAGVAFGMALVVGAGFWRARRTSVAEPPSAMP
                     SQDAAPAAPAASTPSSYPAPAVQG"
     gene            complement(7692..9788)
                     /locus_tag="VF453_23360"
     CDS             complement(7692..9788)
                     /locus_tag="VF453_23360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX7690662.1"
                     /translation="MSAKYEVHGAVAVITLDNPPVNGLGLETRRAVAEGIERAEDDAA
                     VKAIVITGAGKAFSGGADIREFGSPKALAEPNLLSLIARVEGAAKPVVAAIHTVCMGG
                     GLELSLGCHYRVAAPGASIALPEVKLGILPGAGGTQRLPRAIGVEPALNMIVSGEPVK
                     SELLATIPGQKLFDGVIEGDLLPGAIAFAHEVADARPLPRVRDLPCRHPDGDAYFGFA
                     RNTVKAAAKNFPAPARCVDAVEQATKAKSFEDGLKYEREAFANLMLTPESKALRHAFF
                     AERAASKIPDVPESTPVREIAKVAIVGAGTMGGGIAMNFLNAGTPVVILETKQEALDK
                     GVATIRRNYEAQVRKGKLAEDKYAGRMALLSTTLSYAELADADLVIEAVFEDIEVKKS
                     VFEQLDAVMKPGAILASNTSTLDLDRIAGFTKRPQDVIGMHFFSPANVMKLLEVIRGA
                     KTAPDVLATVMKLGKKIRKTCVVSGVCDGFIGNRMIEQYSRQAGFLLEEGCTPQQVDR
                     AIEKFGFAMGPFRMGDLAGNDVGWYIRKRRYQEKPGLRYSKTADLLCEMGRFGQKTGA
                     GWYDYQPGKRDAIPSPVVGEMIERHRASLGVAPRRISDDEIVHRLVYALVNEGARILE
                     EGIALRASDIDMVYLTGYGFPLWRGGPMNYADTVGLWNVAQAMKRFAKNPQDDASFWQ
                     PAPLLARLVADGKSFS"
BASE COUNT         1318 a         3643 c         3553 g         1487 t
ORIGIN      
        1 tgcgctgcgc gcgcagcagg tccagcgcca tgatgttgcc ggcgccctcc cagatcgagt
       61 tcagcggcat ctcgcggtag atgcgcgcca tcacgccctc gcactcgcct tccacgtagc
      121 cattgccgcc gaggcactcc atcgcctcct gcgcgaaatg gctgccgcgc ttgcagaccc
      181 agaacttcgc cgcaggcgtg agcaggcggc gcatcagcga ctcgtgctcg cgcgcggccg
      241 ccggtgccgc ggcgtcctcg ctcgcgtcga ccgcgcgcgc gacgcgcaac gcgagcgccg
      301 tggcggcctc gctctcgagc gccaggtcgg ccagcacgtt gcgcatcagc gcatgttcat
      361 caaggcgcct gctgaaggcg tggcgattgg ccgcgtggtg cagcgcgatc gacagcgcgc
      421 cgcgcatcag ccccgccgtg ccgagggcgc agtccaggcg cgtgagcgcg cccatcgcga
      481 ggatctgcgg cagcccgcgg ccctcgtcgc ccacgcgcca ggcgaaggcg tcgacgaact
      541 cgacctccga gctcgcgttg gccttgttgc cgagcttgtc cttcaggcgc tggatgcgca
      601 ccgcgttcaa ggccccgttt ggcgagccgt cgggcagccg ccgcggcagg aagaagcagg
      661 tcgggccagc gggagcctgg gcgacgacca ggaaggcatc gctcatcggc gccgacatga
      721 accacttgtg gccaacgagc cgccagcgct cgccccaggc gtcgtcgccg tcgcgcaccg
      781 cgcgcgtcgt gttggcgcgc acgtcggagc caccctgctt ctccgtcatg cccatgccca
      841 tcgtcacggc ggtcttggcg gtggggtcga tgaagcgctc gtcgtactcc gggctcgcga
      901 gcagtccgcc gaacgcctgc tgcagcgccg agttcgcgcg cagcgccggc gtcaccgcgt
      961 acgtcatcga gaccgggcac agcaccgagg gctcgagctc ggtgaacagc atgaagccgg
     1021 ccgcgcgctc gacgtggctc ggcccgccgg ccgcccacgg cgcgccgtgc aggcggtggc
     1081 gcagcgcggc gccgagcagc acgtggtagc tcgggtggaa ctcgacctcg tcgatgcgcc
     1141 ggcccgtacg gtcatgggta tgcagcgacg gcccgtgcag gttggcgagc cgcgcgtgcg
     1201 cctgcatgcg cgcgctgccg gcctcggcgc ccagtgccgc gagcgccgtc gtgtccacgc
     1261 ccggccggtt gaacgcgacc gcgtcgcgca gcgcggcgtt gcgctcgaag aggttgacgt
     1321 cctgccaggg cggcacctgg ttgaagacct catgggtcgc accatggctg cttgcgtcgt
     1381 cgaagttcat gcgatgcctc cgtcctgtcg ggccgcgacg cgggccgctt tcgggcggcc
     1441 cggcgcgggt catgatcgcc ccgcccgctc catgtccacg tgggggatgt cgtcctcgag
     1501 gtacgcctcg cccaccgtca cgtagccgtg ccgtccgtag aaggcctgca ggtgggcctg
     1561 cgcgccgatg cggttggcgg cgccgggcca ggtcgcgtcg cagcggcgca gggcctcggc
     1621 gatcagctcg tggcccagac cggtcccgcg ctgcgacgcg tccaccacca cgcgcccgac
     1681 cgagggcacc tcgtacttgc gccccggatc gaccacgcgc acgtaggcga gcagccggcc
     1741 gtcctcgccg cggcccagca ggtgccaggc gtggcggtcg agcccgtcga cgtccaggta
     1801 cgcgcagcgc tgctcgacga cgaagatcgc cgcgcgcagg gccagcgcgt cgtacacgtc
     1861 gtcggccgac aggccctcga accgcgtcca gttccaggag atcgacatca cgcgagcttc
     1921 acgagttgct tgccctggtt gcggcccttg agcaggccga agaaggcctc gggcgcgttc
     1981 tccaggccgt gcgcgatcga ctcgcggtac ttcagcctgc ccgtcgcgac cagcgtgccg
     2041 agctccttga gcgcctcggg ccacacctcc atgtgctcgc tgacgatgaa gccctcgatc
     2101 cgcatgcggt tggtgaggat cagctgcggc agcgtcatcg ggatcggctc gccgttgtag
     2161 ccggcgatca tcccgcacat cgcgacgcgc gcgaacgcgt tggcgcgcag catcaccgcg
     2221 tccagcagca ggccgccgac gttctcgaag tagccgtcga tgccttgcgg gcacgcggcc
     2281 ttgagcgcgc cgctcatcgc cttgaggtcg gtgtgctggc ggtagtcgat gcatgcgtcg
     2341 aagccgagat cctcgacgac gtggcggcat ttctcggcac cgccggcgat gcccaccacg
     2401 cggcagccgc gcgccttggc gagctggccc accgcgcccc cgaccgcgcc cgccgcggag
     2461 ctcacgacca ccgtctcgcc cgccttgggc gcgatgatct tcacgaggcc gtaccagccg
     2521 gtcacgccgg gcatgccgac ggcgccgagg taggccgaga gcggcacgtg cgtggtgtcg
     2581 accttctgca ggaccccgcg ctggccggcg tccacgaccg cgtactcctg ccagccgccc
     2641 atgcccacga ccttgtcgcc gacctggaag tgcgggttgc gcgactcgac cacctcgccc
     2701 gcagtgccgc cgatcatcac ctggtcgagc ggctgcggcg cggcgtagct cttggcgtcg
     2761 ttcatgcggc cgcgcatgta gggatcgagg ctcagccact cgtggcgcac cagcacctgg
     2821 ccgtcggcca gggggcccag gggctgttcg acgagccgga agttgctcgc ggcgggctcg
     2881 ccctcggggc gcgaggcgag ctggatctgg cggttgacga tggtcatgcg ggtctcctgg
     2941 gaacgtgtac ggggtatcgg tacggctgga agacgggatt gtcagggccg gcgcagggtg
     3001 cgcaggtact tgaaggtgcc ggtggcgtgg gcgcacagcg cgccggtctc gtcgcgcacg
     3061 ctgccctcgc agaacgcggt gctcgcgccg ccgtgcagca gcgtgccgaa cgcgacgagc
     3121 cggccgtgcg ccggccgcat gaagctcgtc ttcatctcca cggtgacgat gcccggcgag
     3181 ccgtgctccg acggatcgcg cacgctgcgc gccgcgtgcg ccatcgcgac gtcgagcagc
     3241 gtcatcagca cgccgccgtg cgcgacctgc cacgagttga gcatgtcggg acgcaggtcg
     3301 aggctcacct cggcatggcc gccgtcgaag cgccacagcc gcatcccgag cgcctcgacg
     3361 aaggggatgt cgacggcgaa cggaatcggc tggcgatcgt cgtccgtcat gtcagccgcc
     3421 atgcacggcg ctgacgccgc cgtcgaccgc gatcacctgg ccggtgatgt gcttgcccgc
     3481 gtcgctggcc agcagcagcg cggcgccctt caggtcctcg tcgtcgccga tgcgtcgcag
     3541 cggcgcatgc gcggccatcg cgtcctcgcc gacggcctgc agcaggcccg cggtcatgcg
     3601 gctcgggaag aagcccggcg ccagcgcgtt gacctggatg ccgtaggcgc cccattcgcc
     3661 ggcgagggcg cgcgtgaagt tgatgaccgc cgccttgctc gtgttgtagg cgacggtctt
     3721 catcgccccg gcggcgttgc ccgacaggcc cgcgatcgac gcgacgttga tcacgcggcc
     3781 gcgccggcgc gggatcatgc tcgcgcggcc gaccgcctgc gtgagcagga agatcgcgcg
     3841 cacgttgagg ttcatcacct tgtcccaggc ctcgagcgga tgctcctccg cgggcgcgcc
     3901 ccaggtcgcg ccggcgttgt tcaccagcac gtcgacctcg cccaggcgct cgcgcgcggc
     3961 ggcggccagg cgctcgacct cggcgggctg gctcgcgtcg gcagcgatcc agtcggcctc
     4021 ggcgccgcgc tcgcacagcg ctgcgacggc ggtctcgagc tcgggcgcct tgcgcgcgga
     4081 cagcagcacg cgcgcgccgg cctcgccgag cgcctcggcg atctgcaggc ccagcccgcg
     4141 cgagccgcct gtgacgaggg ccgtgcggcc cgagaggtcg aacagctgct gcacgcggcg
     4201 gcgctcgtcg cgcggaacgg cggggatggc ggaggaagtc atcgcgatac ggggtccggg
     4261 catgaggtca gaacgcatcc tcgggcatgt ccaggcaggt gcggtcgcgc gtctcgacga
     4321 cgcgcagcca ggcatcgacc ttcggcagct cgaagtgcat gaagtagtcg agcgccgcca
     4381 ggcgcccacg cgaggcggcg tcgccggcgc cgcgatcgac gaccgccgcg gcgacgtcca
     4441 gccacagcca ggccagcacg acgtggccga aggcctgcag gtagggcgtg gcgttggcga
     4501 gcgcctcctc gggcgccgag gtcgcccagg cggcgcgcgt cgcggcgccg agcttctgca
     4561 gcgcgcgcgc gagcgcgttg gcgtgcatgg ccaggcgctc gtgatgcaac gcgcgcgcga
     4621 cggtcgcgtc gatgcggtgc gcgagcagct tcagccccgc gccgccctgc atcacggcct
     4681 tgcgcccgag caggtccagc gcctgtatgc cgtgcgtgcc ctcgtggatc atgttcaggc
     4741 ggttgtcgcg ccagtgctgc tcgacgggga agtcgcgcgt gtagccgtag ccgcccagca
     4801 cctggatcgc gaggctgttg gcctccagcg cccactcgct cggccagctc ttggcgatgg
     4861 gggtgagcat ctccagcagc agtgccgctt gcgccgcggc ctgcgcgtcg cccgtgtgct
     4921 cctcgtcgac cagccgcgcg cagtacagcg cgagcgccat gccgccttcg acgatcgcct
     4981 tctgcgcgag cagcatgcgc ctgacgtccg cgtgctcgat caggcgcacg ggcggcgccg
     5041 cggcgtcctt gccgccggcg ccgacgcagc ggccctgcgt gcgctcgcgc gcatacgcga
     5101 ggctcgcgtc gtagcccgcc gccccgagca tggtcgcgcc caggccgacg ccgatgcgcg
     5161 cctcgttcat catgtggaac atgcagcgca ggccgtcgcc gggtgccccc acccggtatc
     5221 cgatcgcgcc gccctgctcg ccgaagttca gcagcgcatt cgtcgtgccg cggtagccga
     5281 gcttgtggtt caggccggcg aggcgcacgt cgttgcgctc gcccgtcagg cggccgtccg
     5341 catcgacgag ccacttcggc acgatgaaca gcgagatgcc ccgggtgccg ggcaccggct
     5401 tgccgtccgc tcccgggatc ttggcgagca ccaggtgcac gatgttggcc gtgatctcgt
     5461 gctcgcccgc cgagatccac atcttgttgc cggccaggcg gtaacgcggg ccgagcggat
     5521 cggcgtcgaa gccggcgccg tcgggcacgg cgcgcgtggc gatgtcgccg agcgacgagc
     5581 ccgcctgcgg ctcgctcagg cacatcgtgc cgaaccattc gcccgcgaac tcgcgcgccg
     5641 cgaacacctc gcgctggcgc ggcgtcccgt gggccatcag caggcgagcg ttcccggtgg
     5701 tcagcatcgc gtaggcaccc agcgcgatgc aggcctgcga gaagaacgcg ttgcccgcct
     5761 tctcgacgac gcagggcagc tgcatgccgc cgacctccgc gtcctgcgag gccgcgagca
     5821 tcccgctggc cgcgtacgcc cgcagagcgt cgtgcgtggc ccgcgggagc gtcacgcgct
     5881 cgccgtcgaa cgcgggctcc tcggtgtcga cgagccggtt gaacggcgcg aacttctcgc
     5941 gcgccagccg ctcgcacagg tcgagcgcgg cgtcgaaggt cgcgcgcgaa tgctccgcga
     6001 agcgcggacg cgcgcacagc gcgtcgacgc gcagccagtc gtggagcagg aagtcgagcg
     6061 tctcgcgagt cgtcacgggt tctcccatca tctccggcgc tgccgctcgg gcgtgtcagg
     6121 acagacacgg gcaagcggcg gatccggctt cgccgggccg ctcgcgcacg cccctccggg
     6181 gggcagggat cgctagcgac cgtgggggct cacagcactt cgaacacgcc ggccgcgccc
     6241 atgccgccgc cgatgcacat cgtcacgcag accttcttcg cgccgcggcg ccggccttcg
     6301 accagcgcgt ggccggtcag gcgctggccg ctgacgccgt agggatggcc gaccgcgatc
     6361 gcgccgccgt tgacgttcag gcgatccatc gggatgccca gcgtgtcggc gcagtacagc
     6421 acctgcacgg cgaaggcctc gttgagttcc cagaggtcga tgtcgcccac cgtcagcccc
     6481 aggcgcttga gcaccttggg caccgcgtac acgggcccga tgcccatctc gtcgggctcg
     6541 cagccggcga ccgcgaagcc gaggaagcgg cccagcggct tcaggccctt gcgctcggcg
     6601 tactggctcg agacgaccac gcaggcgccg ccgccgtcgc tgaactggct cgcgttgccc
     6661 gcggtgatca cgccgccggg gacggcggtg cggatgtcct tgatgccctc gtaggtcgtg
     6721 ccctcgcgca ggccctcgtc gaccgcgacc gtgacctgcc tggtgcggat gccgttcttc
     6781 ggatcggcga cgccggcggt caccgtgatc ggcgcgatct cctcggcgaa cctgcccgcg
     6841 gcctgcgcgg cacacgcctt ctgctggctc tcggcgccgt agcggtcctg gcgttccttg
     6901 gagatcttgt agcgcttggc gacgttctcc gcggtctcca gcatcgacca gtagatctcc
     6961 ggcttgttct tcgcgagcca catgtcgcgc agcatgtgct ggttcatctc ctgctgcacg
     7021 cacgagatgc tctccacgcc gccggccacg tagacgtcgc cctcgcccgc gatcacgcgc
     7081 tgcgcggcca tcgcgatcgt ctgcaggccg ctggagcaga agcggttgac cgtcacgccc
     7141 gccaccgaga ccggcagccc ggcacgcagc gcgacctggc gcgcgatgtt ggcgccggtc
     7201 gcgccctcgg gattggcgca gcccatcagc acgtcctcga cctccgcgcc gtcgatgccg
     7261 gcgcgctcga tcgcggcgcg cacggcatgg ccgccgagct ccgcgccgtg ggtcatgttg
     7321 aaggcgccct tccagctctt ggcgagcggc gtgcgggcgg tcgagacgat gacggcttcg
     7381 gtcatgggaa ggctcctgcg tcgagagtgg gatcgcgtcg gtcgggtcgg atcggcgatg
     7441 gtgtcggtga tgcgtcagcc ctgcacggcc ggcgcgggat agctggaggg ggtcgaggcg
     7501 gcgggcgcgg ccggcgcggc gtcctgcgac ggcatggcgg acggcggctc ggcgacgctc
     7561 gtccggcgcg cgcgccagaa gcccgcgccg acgaccagcg ccatgccgaa cgcgacgccc
     7621 gccgtgacga gcatcgcgcg cttgaggaga ccggccacca tgggacgcgc ctcgccgccc
     7681 gccgcctggc gtcagctgaa ggacttgccg tcggccacca ggcgcgccag cagcggcgcc
     7741 ggttgccaga acgacgcgtc gtcctgcgga ttcttcgcga agcgcttcat cgcctgcgcg
     7801 acgttccaca ggcccacggt gtccgcgtag ttcatcgggc cgccgcgcca gagcgggaag
     7861 ccatacccgg tgaggtagac catgtcgatg tcggaagcgc gcagggcgat gccctcctcc
     7921 aggatgcgcg cgccctcgtt gaccagcgcg tacaccaggc ggtgcacgat ctcgtcgtcg
     7981 gagatcctgc gcggcgccac gcccagcgac gcgcgatgcc gctcgatcat ttcacccacg
     8041 acgggcgacg ggatcgcgtc gcgcttgccc ggctggtagt cgtaccagcc cgcgccggtc
     8101 ttctggccga agcggcccat ctcgcacagc aggtcggcgg tcttcgagta gcgcaggccg
     8161 ggcttctcct ggtagcgccg cttgcggatg taccagccga cgtcgttgcc ggcgaggtcg
     8221 cccatgcgga acgggcccat cgcgaagccg aacttctcga tcgcgcggtc gacctgctgg
     8281 ggcgtgcagc cctcctcgag caggaagccg gcctggcggc tgtactgctc gatcatgcgg
     8341 ttgccgatga agccgtcgca aacgcccgag accacgcagg tcttgcggat cttcttgccc
     8401 agcttcatga ccgtggcgag cacgtccggc gcggtcttcg cgccgcggat gacctccagc
     8461 agcttcatca cgttggccgg gctgaagaag tgcatgccga tcacgtcctg cgggcgcttc
     8521 gtgaacccgg cgatgcggtc caggtccagc gtcgaggtgt tgctcgcgag gatcgcgccc
     8581 ggcttcatca ccgcgtcgag ctgctcgaac accgacttct tgacctcgat gtcctcgaac
     8641 accgcctcga tcacgaggtc cgcgtcggcg agctcggcgt acgacagcgt ggtggacagc
     8701 agcgccatgc gcccggcgta cttgtcctcg gccagcttgc ccttcctgac ctgcgcctcg
     8761 tagttcctgc ggatggtcgc gacgcccttg tccagcgcct cctgcttcgt ctcgaggatg
     8821 acgaccggcg tgcccgcgtt gaggaagttc atcgcgatgc cgccgcccat cgtgccggct
     8881 ccgacgatcg cgaccttggc aatctcgcgc acgggcgtcg actcgggcac gtcggggatc
     8941 ttggaggcgg cgcgctcggc gaagaacgcg tggcgcagcg ccttgctctc gggcgtgagc
     9001 atcaggttgg cgaaggcctc gcgctcgtac ttcagcccgt cctcgaagga cttcgccttc
     9061 gtcgcctgct cgaccgcgtc gacgcagcgc gccggcgcgg gaaagttctt cgcggccgcc
     9121 ttgaccgtgt tgcgcgcgaa gccgaagtag gcgtcgccgt ccgggtggcg gcacggcagg
     9181 tcgcgcacgc gcggcagggg gcgggcgtcg gcgacctcgt gggcgaacgc gatcgcgccc
     9241 ggcagcaggt cgccctcgat caccccgtcg aacaacttct ggccggggat cgtcgcgagc
     9301 agttcgctct tgaccggctc gccgctgacg atcatgttga gcgccggctc gacgccgatg
     9361 gcgcgcggca ggcgctgcgt gccgcccgcg ccgggcagga tgccgagctt gacctccggc
     9421 agcgcgatgc tcgcgcccgg cgccgcgacg cggtagtggc agcccagcga gagctccagc
     9481 ccgccgccca tgcacaccgt gtggatcgcg gcgaccacgg gcttggcggc cccttcgacg
     9541 cgcgcgatca ggctcagcag gttgggctcg gccagcgcct tggggctgcc gaactcgcgg
     9601 atgtcggccc cgcccgagaa cgccttgccg gcgccggtga tgacgatggc cttcaccgcg
     9661 gcgtcgtcct cggcgcgctc gatgccctcg gcgaccgcgc gccgcgtctc caggccgagg
     9721 ccgttgaccg gcgggttgtc gagcgtgatg acggcgaccg cgccgtggac ttcgtacttc
     9781 gcactcatgg ggtgtctccg tgctgaccgg cggcgggcgc cagaaaatag aacgatcgtt
     9841 cgattctagg atcgtttttc ggcgggcgcg tgtcccggcc gcgacaaggc cgggacaacc
     9901 cggggtcgcc ccggcgcggc cgcgaacgcc agccagcata cgacgagacg accggcggcg
     9961 cgccggccgc cgccgcgtcg gccgcagggg cagtctccgg g
//
LOCUS       DASVLN010000997         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_268208, whole genome shotgun sequence.
ACCESSION   DASVLN010000997 DASVLN010000000
VERSION     DASVLN010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268208"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..605)
                     /locus_tag="VF453_23365"
     CDS             complement(135..605)
                     /locus_tag="VF453_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEX7690663.1"
                     /translation="MGLEIERKFLVTGDAWRAAPAVAYAQGYLNRDPARTVRVRIAGD
                     AAWLTVKGPTRGATRAEFEYAIPPADAQALLALCDGPPVRKLRRVVEHAGARWEVDEF
                     LDANAGLVVAEIELPAEDAPFERPAWLGAEVTHDARYFNSALAARPYAQWRAEG"
     gene            713..1213
                     /locus_tag="VF453_23370"
     CDS             713..1213
                     /locus_tag="VF453_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7690664.1"
                     /translation="MRTPIRWRKDHAGGALLVAAGIAVVATSLGYGVGSLRQMGSGYF
                     PLALGVVMALMGAVLLATTPWRDTPQPPPGDALAGGEHAHLAGPVWQPRGWLCIVGAL
                     GAFVVLGDHGGLVPASFASVFIAALGDRRNGPRDAALLAAGMTVFGVIVFHYGLHLLL
                     PLFQWA"
     gene            1218..2717
                     /locus_tag="VF453_23375"
     CDS             1218..2717
                     /locus_tag="VF453_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7690665.1"
                     /translation="MHDALQQLVFGFGIALQPQNLMWSAFGVLVGNLVGVLPGMGPMS
                     TISMLLPLTYVMGPVPAILMLAGIFYGSQYGGAIGAILLNLPSHPPHAVTCLDGYPLT
                     RQGKGGTALGITMMASLFAASVGIVVMIFLSQPLVELAGRFGPADVCAVMLLGLLSCA
                     TMARGSPVKGVAMTVLGLLCGVAGTDEISGNARFTFGLTELRDGFELGGLTMGLFGVA
                     DFLVSVNRTTVNPSHAKVRMRDMRPSLAEIRQSFWPMVRGTAVGTAFGALPGTGPTIT
                     TFIAYALERRWSKTPERFGRGALEGVASPEASNHSKTQVDFIPTLSLGIPGDAVMALI
                     LGALILKGVQPGPQLIGDHPDIFWGLVASFWIGNVLLVILNVPLIGVWVRMLRVPYRL
                     LFPSALFFIAVGVYSARNSLFDVGLVAAVGIVGALLLLLDFPLAPVVLGFVLGPMVEA
                     NFRRAMLIAHGDLAVFVQRPVAAIFLAMSIAVVAVQAVAAWRRPLPARQ"
BASE COUNT          305 a         1016 c         1045 g          466 t
ORIGIN      
        1 ggcggcgttc tcgcgcagca cctcgacggt ggcgcggccg acggtgccga agcccagcat
       61 gccgatgttc atgtgcggtg ttcccttgca gtggttggcg tcaggcggcg attgtctccg
      121 acgcggcgcc gtcgtcatcc ctcggcgcgc cactgcgcgt acggccgcgc cgcgagcgcc
      181 gagttgaagt agcgcgcatc gtgggtgacc tcggcgccca gccaggcggg gcgctcgaag
      241 ggcgcgtcct cggcgggcag ctcgatctcg gcgaccacca ggccggcgtt ggcgtcgagg
      301 aactcgtcga cctcccagcg cgcgccggcg tgctcgacga cgcgccgcag cttgcgcacg
      361 ggtggcccgt cgcacagcgc gagcagcgcc tgggcgtcgg cgggcgggat cgcgtactcg
      421 aactcggcgc gcgtcgcccc gcgggtgggc cccttgacgg tcagccacgc cgcgtcgccg
      481 gcgatgcgca cgcgcaccgt gcgcgccggg tcgcggttga ggtagccctg cgcataggcg
      541 accgcgggcg cggcgcgcca ggcgtcgccc gtgacgagga acttgcgttc gatttcgagg
      601 cccatggcgg gctcctggcg agtggggatg cgggattgtc ggcgagcgcg cggcgtcggc
      661 gcggccgagc ggttagagtc gggcgcttca cgtggaggca aggtcgactc tcatgcggac
      721 tccgatccga tggcgcaagg atcacgccgg cggcgcgctg ctcgtcgcgg ccggcatcgc
      781 ggtggtggcg acgagcctgg gctacggcgt cggctcgctg cggcagatgg gctccggcta
      841 tttcccgctc gcgctcggcg tggtgatggc gctgatgggc gcggtgctgc tggcgacgac
      901 gccctggcgc gacaccccgc agccgccgcc cggcgacgcg ctggccggcg gcgagcacgc
      961 ccacctcgcc ggccccgtgt ggcagccgcg cggctggctg tgcatcgtcg gcgcgctcgg
     1021 cgcgttcgtc gtgctgggcg accacggcgg gctcgtgccg gcgtcgttcg cgtcggtgtt
     1081 catcgcggcg ctgggcgacc gccggaacgg cccgcgcgac gcggcactgc tggccgcggg
     1141 catgacggtc ttcggcgtga tcgtcttcca ctacggcctg cacctgctgc tgccgctgtt
     1201 ccagtgggct tgagcgcatg cacgacgccc tccagcaact ggtcttcggc ttcggcatcg
     1261 cgctgcagcc ccagaacctc atgtggtcgg ccttcggggt gctggtgggc aacctcgtgg
     1321 gcgtgctgcc cggcatgggg ccgatgtcca ccatctcgat gctgctgccg ctcacctacg
     1381 tgatgggccc ggtgccggcg atcctgatgc tggcgggcat cttctacggc tcgcagtacg
     1441 gcggcgcgat cggcgcgatc ctgctgaacc tgccgagcca cccgccgcac gcggtgacct
     1501 gcctcgacgg ctacccgctc acgcgccagg gcaagggcgg cacggcgctc ggcatcacga
     1561 tgatggcctc gctgttcgcg gcgtcggtgg gcatcgtcgt gatgatcttc ctgtcgcagc
     1621 ccctggtcga gctggccggg cgcttcggcc ccgccgacgt ctgcgccgtc atgctcctgg
     1681 gcctgctgtc gtgcgcgacg atggcgcgcg gctcgccggt caagggcgtg gcgatgacgg
     1741 tgctcggcct cctgtgcggc gttgccggca cggacgagat cagcggcaac gcccgcttca
     1801 ccttcggcct gaccgagctg cgcgacggct tcgagctcgg cggcctgacg atggggctgt
     1861 tcggcgtcgc cgacttcctc gtcagcgtca accgcaccac cgtcaacccg tcgcacgcga
     1921 aggtgcgcat gcgcgacatg cggccgagcc tcgccgagat ccgccagtcg ttctggccga
     1981 tggtgcgcgg cacggcggtc ggcacggcgt tcggcgcgct gcccggcacg ggccccacga
     2041 tcacgacctt catcgcctac gcgctggagc gccgctggtc gaagacgccc gagcgcttcg
     2101 ggcgcggcgc gctggagggc gtcgcgtcgc ccgaggcgtc caaccactcg aagacgcagg
     2161 tcgacttcat cccgacgctg tcgctgggca tcccgggcga cgccgtgatg gcgctgatcc
     2221 tcggcgccct gatcctcaag ggcgtgcagc ccgggccgca gctgatcggc gaccaccccg
     2281 acatcttctg gggcctggtg gcgagcttct ggatcggcaa cgtgctgctc gtgatcctga
     2341 acgtgccgct gatcggcgtc tgggtgcgca tgctgcgcgt gccgtaccgg ctgctgttcc
     2401 cgtcggccct gttcttcatc gcggtgggcg tctacagcgc gcgcaacagc ctgttcgacg
     2461 tcgggctggt cgccgcggtg ggcatcgtcg gcgcgctgct gctcctgctc gacttcccgc
     2521 tcgcgcccgt cgtgctcggc ttcgtgctcg gcccgatggt cgaggccaac ttccgccgcg
     2581 cgatgctgat cgcccacggc gacctggccg tgttcgtgca gcggccggtg gcggcgatct
     2641 tcctcgcgat gagcatcgcc gtggtggcgg tgcaggcggt cgcggcctgg cggcgcccgc
     2701 ttccggcgcg acaatgagcc ccccgcccag ccgttccgaa gggggctcgc accgcggtcc
     2761 gccggacggc ggcggtcttg tgagcgtcgc catgatgtcc atgcctcgtc ccctccgccg
     2821 cgcgatcggc gc
//
LOCUS       DASVLN010000998         8952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_375389, whole genome shotgun sequence.
ACCESSION   DASVLN010000998 DASVLN010000000
VERSION     DASVLN010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8952
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375389"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VF453_23380"
     CDS             complement(<1..269)
                     /locus_tag="VF453_23380"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7690666.1"
                     /translation="MFLVPVSRRAAVRPHGFDRLFDEAFDRFLGHAGLADTDAARRPA
                     LDVAESDAAYVVMLDVPGVAREDVKVSIEGRRVSIAAQARAPQAEA"
     gene            719..982
                     /gene="rpsO"
                     /locus_tag="VF453_23385"
     CDS             719..982
                     /gene="rpsO"
                     /locus_tag="VF453_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7690667.1"
                     /translation="MADINKADIVAAHARGTGDTGSPEVQVALLTARINALTPHFKEH
                     AKDHHGRRGLLKMVNTRKSLLAYLKRTDADRYTALIAKLGLRK"
     gene            1206..3338
                     /gene="pnp"
                     /locus_tag="VF453_23390"
     CDS             1206..3338
                     /gene="pnp"
                     /locus_tag="VF453_23390"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7690668.1"
                     /translation="MSMFNKVTKTFQWGNHTVRLETGEIARQSTGAVVVDIEGTVVLA
                     TVVAKREAKPGQDFFPLTVDYIEKTYAAGKIPGSFFKREGRPSEHETLTSRLIDRPIR
                     PLFPEGFFNEVQVVIHVMSLNPEVDADIAAMIGSSAALAISGIPFAGPIGAARVGYIN
                     GQYVLNPGKTERTNSQMDLVVAGTESAVLMVESEADQLPEDVMLGAVVFGHDQGKIAI
                     NAIHELVRDAGKPVWTESGEWTPPAKDEPLIAKVEALAKAKLEAAYQIRSKQSRTQAC
                     RAAYAETKEALKAEGVEFDGVKVDALLFDIEAKIVRGQILAGEPRIDGRDTRTVRAIE
                     IRAGVLPRAHGSAVFTRGETQAVVATTLGTERDAQTIDALAGEYSERFMLHYNMPPFA
                     TGETGRVGSPKRREIGHGRLAKRALVAVMPSKEDFPYSVRVVSEITESNGSSSMASVC
                     GGCLSLMDAGVPLKAHVAGIAMGLIKEGNRFAVLTDILGDEDHLGDMDFKVAGTTQGI
                     TALQMDIKIQGITKEIMQVALAQAKEARLHILGKMVEAVGGASTEVSQFAPRLYVMKI
                     NPEKIRDVIGKGGATIRALTEETGTQIDIAEDGTITIASTDADRAAQAKKRIEEITAE
                     VEVGKVYEGPVTKILDFGALVNLLPGKDGLLHISQIAHQRVEKVTDFLKEGQIVKVKV
                     LETDDKGRVKLSMKALLDRDQDAGQQPQ"
     gene            3391..4380
                     /locus_tag="VF453_23395"
     CDS             3391..4380
                     /locus_tag="VF453_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX7690669.1"
                     /translation="MRAIEIAGFGGPEVLREAVRPRPVAGAGEALVRVSVSGVNRPDV
                     LQRKGHYAPPAGASDLPGLELAGTIEGGDEAALAAAGFQVGDRVCALVAGGGYAEFCV
                     VPVPQLLPVPAGFSDVEAAALPETFFTVWSNVFDRGRLAAGEWLLVQGGASGIGTTAI
                     QLAKAFGAKVIATAGSASKCARVLELGADHAIDYKAEDFGVRALEFTGGRGVDVVLDM
                     VAGDYVARELACLAEDGRVVIIAVQGGTKAQIDAGLVLRKRLVVTGSTLRPRPVAFKG
                     AIAAALREKVWPLLEARRVQPVIQQVFPAAEAARAHAAMEQGDHVGKLLLAWR"
     gene            4412..5152
                     /gene="tpiA"
                     /locus_tag="VF453_23400"
     CDS             4412..5152
                     /gene="tpiA"
                     /locus_tag="VF453_23400"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7690670.1"
                     /translation="MRKKLVAGNWKMHGSHPQNAELLAGILAARPFGCDVAVCVPFPY
                     LSEVAVALAGSDIRWGAQDCSPHAQGAYTGEVSAAMLAEFGCRYAIVGHSERRAMHAE
                     SDQLVADKAKAVLGRGLTPIVCVGETLAQREQGVAAEVVKRQLSAVIHALGHCVGEMV
                     VAYEPVWAIGTGKTASPEQAQEMHALIRAQLHAATERADAMTLLYGGSVKPDNAATLF
                     AQPDIDGGLIGGAALKAADFIAICRAAG"
     gene            5202..5627
                     /gene="secG"
                     /locus_tag="VF453_23405"
     CDS             5202..5627
                     /gene="secG"
                     /locus_tag="VF453_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX7690671.1"
                     /translation="MQIWLNLLFILQILAAVTMIGLVLLQHGKGADMGASFGSGASGS
                     LFGATGSANFLSRSTAICATIFFAATLGLAGIATNRVTVHAPTTSSDNALTQALPASA
                     AATVVVPTASAPSAGASVAASAATTASAPATGSQGVPTR"
     gene            5790..5874
                     /locus_tag="VF453_23410"
     tRNA            5790..5874
                     /locus_tag="VF453_23410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5824..5826,aa:Leu,seq:gag)
     gene            6110..6469
                     /locus_tag="VF453_23415"
     CDS             6110..6469
                     /locus_tag="VF453_23415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX7690672.1"
                     /translation="MDLTAYLPVILFILVGVGVGVAPQVLGFVLGPSRPDAQKNSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVALKDIGQTGFWAMMIFLAI
                     LVVGFIYEWKKGALDWE"
     gene            6491..6976
                     /locus_tag="VF453_23420"
     CDS             6491..6976
                     /locus_tag="VF453_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX7690673.1"
                     /translation="MAKGNEGLLKEGFVTTQVDKLVNWSKTGSLWPMTFGLACCAVEM
                     MHAGAARYDIDRFGMLFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVLSMG
                     SCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGILQLQAKVRRENTIAR
                     A"
     gene            7020..7619
                     /locus_tag="VF453_23425"
     CDS             7020..7619
                     /locus_tag="VF453_23425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7690674.1"
                     /translation="MTQRLDALQAALEAALGARIAQFKRERGEISITVKAADSLAVAT
                     QLRDDPTLGFEQLIDVCGVDFSSYKEQPWDGPRYCAVTHLLSHRHNWRLRLKVFAPDD
                     DLPVVPSLTPIWNSANWYEREAFDMYGILFEGHDDLRRILTDYGFIGHPMRKDFPVTG
                     DVEMRYDAEQRRVLYQPVTIEQREITPRVVREDNYGGLR"
     gene            7702..>8952
                     /locus_tag="VF453_23430"
     CDS             7702..>8952
                     /locus_tag="VF453_23430"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX7690675.1"
                     /translation="MAEIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAETKTFIQSLPYMDRLDYVSMMSNEQAYCLAIERLLGIDVPLRAQYIRVMFA
                     EITRLLNHLMWLGAHGIDCGAMNVLIYCFREREDLFDMYEAVSGARMHAAYFRPGGVY
                     RDLPDAMPQYKASRIHNAKAIARMNENRQGTLLDFIEDFTKRFPKHVDEYETLLTDNR
                     IWKQRTVGIGVVSPERARQLGFTGPMLRGSGIEWDLRKKQPYDVYDKMDFDVPVGVNG
                     DTYDRYLVRVEEMRQANRIIAQCVKWLKANPGPVITANHKVAPPSRTEMKSNMEELIH
                     HFKLFTEGFHVPEGEQYAAVEHPKGEFGIYLISDGANKPYRLKIRAPGFAHLAAMDEM
                     SRGHMLADAVAVIGTMDIVFGEIDR"
BASE COUNT         1509 a         3151 c         2961 g         1331 t
ORIGIN      
        1 gcctcggcct gcggcgcgcg cgcctgggcg gcgatgctca cgcgacggcc ctcgatcgag
       61 accttgacgt cctcgcgcgc gacgcccggc acgtcgagca tgacgacata cgcggcatcg
      121 gactcggcga cgtcgagcgc ggggcggcgc gcggcgtcgg tgtcggccag tccggcatgg
      181 ccgaggaagc ggtcgaaggc ctcgtcgaac aggcggtcga agccatgggg gcggacggcg
      241 gcgcggcggg aaacgggaac gaggaacatg cggtttctcc ttggaggaag aggggggtgc
      301 gctcggtgcc gcgatgtcgg ttcgacacgg cggcgtcggc gcatgacccg gtacttggtc
      361 gtcgcccgat gcgtttcaag gggcgccccg cgcgccaaat ttttccgctc gcgggcttga
      421 attcggtgct gtccgctcca gccgccggcc ggcggccgcg ccgggatttc gggctgctcg
      481 tggcaaggcc cgcggcatgt tttcaggtat actttccggc ttgcgtcgaa ttcggctccc
      541 gttgccggga acgaaggcgg cgcatccagc acgccccggg tcggtttcac ccgggtgcgc
      601 aagtcgtcaa aggaaaccga gcaggcccgc cgtactcatg ccgaggcgcc gggtcgcgaa
      661 atccgggcct gcccgggcaa gcgctgccgg tttccgatct tcacctgaag gaaatcccat
      721 ggcagacatc aacaaggcag acatcgtcgc ggcgcacgcc cgcggtaccg gcgacacggg
      781 ctcccccgaa gtccaggtcg cgctgctgac cgcccgcatc aacgcgctga ccccgcactt
      841 caaggaacac gcgaaggacc accacggccg ccgcggcctg ctgaagatgg tcaacacgcg
      901 caagagcctg ctagcctacc tgaagcgcac cgacgccgac cgctacaccg cgctgatcgc
      961 caagctcggt ttgcgcaagt aagccatcca tgacgcgaga gagcctgtct ggaacctcca
     1021 gcacaggctc tttgcgtttg gagtccggtt cgcacgcaat ggcgccgatg tgtcattcca
     1081 ccgggattcg cgcggcgcgc cgcgtggccg agtcccgctg gaatggcatc acgccactcg
     1141 tcgaacaggc cgctcccgtc tccacgccac gacacggcgt ccaataaaca tcggagaaaa
     1201 acgacatgag catgttcaac aaggtcacca agacgttcca gtggggcaac cacaccgtcc
     1261 gtctcgagac gggcgagatc gcgcgccagt ccaccggcgc cgtcgtcgtc gacatcgaag
     1321 gcaccgtcgt gctggccacc gtcgtggcca agcgcgaggc caagccgggc caggacttct
     1381 tcccgctgac ggtcgactac atcgagaaga cctacgccgc cggcaagatc ccgggcagct
     1441 tcttcaagcg cgaaggccgc ccgagcgagc acgagacgct cacctcgcgc ctgatcgacc
     1501 gcccgatccg cccgctgttc cccgagggct tcttcaacga ggtgcaggtc gtcatccacg
     1561 tgatgtccct gaacccggaa gtcgatgccg acatcgccgc gatgatcggc tccagcgccg
     1621 cgctggcgat ctccggcatc ccgttcgccg gcccgatcgg cgccgcgcgc gtgggctaca
     1681 tcaacggcca gtacgtgctg aatccgggca agaccgagcg cacgaactcg cagatggatc
     1741 tcgtcgtcgc cggcacggaa tcggcggtgc tgatggtcga gtcggaagcc gaccagctgc
     1801 ccgaggacgt gatgctgggc gccgtggtct tcggccacga ccagggcaag atcgccatca
     1861 acgcgatcca cgagctcgtg cgcgacgccg gcaagccggt gtggacggaa tcgggcgagt
     1921 ggacgccgcc ggccaaggac gagccgctga tcgccaaggt cgaggcgctc gccaaggcca
     1981 agctcgaggc cgcctaccag atccgcagca agcagtcgcg cacccaggcc tgccgcgccg
     2041 cctacgccga gaccaaggaa gcgctgaagg ccgagggcgt cgagttcgac ggcgtcaagg
     2101 tcgacgcgct gctgttcgac atcgaggcga agatcgtccg cggccagatc ctggcgggcg
     2161 agccgcgcat cgacggccgc gacacccgca ccgtgcgcgc gatcgagatc cgcgccggcg
     2221 tgctgccgcg cgcccacggc tcggccgtgt tcacgcgcgg cgagacccag gccgtcgtcg
     2281 ccacgacgct gggcaccgag cgcgacgcgc agacgatcga cgcgctggcc ggcgaatact
     2341 cggagcgctt catgctccac tacaacatgc ccccgttcgc gaccggcgag accggccgcg
     2401 tcggcagccc gaagcggcgc gagatcggcc acggccgcct cgccaagcgc gcgctggtcg
     2461 cggtgatgcc gtccaaggag gacttcccgt actccgtgcg cgtcgtctcc gagatcaccg
     2521 agtcgaacgg ctcgtcgtcg atggcttcgg tctgcggcgg ctgcctgtcg ctgatggatg
     2581 ccggcgtgcc gctgaaggcg cacgtcgcgg gcatcgccat gggcctgatc aaggaaggca
     2641 accgcttcgc ggtgctgacc gacatcctgg gcgacgagga ccacctcggc gacatggact
     2701 tcaaggtggc cggcacgacg cagggcatca ccgcgctgca gatggacatc aagatccagg
     2761 gcatcaccaa ggagatcatg caggtcgccc tggcgcaggc caaggaagcg cgcctgcaca
     2821 tcctcggcaa gatggtcgag gccgtcggcg gcgccagcac cgaggtctcg cagttcgcgc
     2881 cgcgcctgta cgtgatgaag atcaaccccg agaagatccg cgacgtgatc ggcaagggcg
     2941 gtgcgacgat ccgcgcgctg acggaagaga cgggcacgca gatcgacatc gccgaggacg
     3001 gcacgatcac catcgcctcg accgacgcgg accgcgcggc gcaggccaag aagcgcatcg
     3061 aggagatcac cgcagaggtc gaggtcggca aggtctacga aggcccggtc accaagatcc
     3121 tggacttcgg cgccctggtc aacctgctgc ccggcaagga tggcctgctg cacatcagcc
     3181 agatcgccca ccagcgcgtc gagaaggtca cggacttcct caaggaaggc cagatcgtca
     3241 aggtcaaggt gctcgagacc gacgacaagg gccgcgtcaa gctgtccatg aaggccttgc
     3301 tcgaccgcga ccaggacgcg ggccagcagc cgcagtgagc ggcgtcgtcg cagctcgtcg
     3361 actcccgtga ccgcgctccc cgcgaccacc atgcgcgcca tcgagatcgc cggcttcggc
     3421 ggtcccgagg tgctgcgcga agccgtgcgg ccgcgacccg tggcgggcgc cggcgaggcg
     3481 ctggtgcgcg tgagcgtctc gggcgtcaac cggcccgacg tgctgcaacg caagggtcac
     3541 tacgcgccgc cggccggcgc ctccgacctg ccgggcctcg agctcgcagg gacgatcgag
     3601 ggcggcgacg aggcggcgct cgcggctgcc ggcttccagg tcggcgaccg cgtctgcgcg
     3661 ctcgtcgccg gcggcggcta cgcggagttc tgcgtcgtcc ccgtcccgca gttgctgccg
     3721 gtgcccgccg gcttcagcga cgtcgaggct gcggcgctgc cggagacctt tttcaccgtc
     3781 tggtccaacg tgttcgaccg cggtcgcctg gccgccggcg agtggctgct ggtgcagggc
     3841 ggcgcgagcg gcatcggcac caccgcgatc cagctggcca aggccttcgg cgccaaggtg
     3901 atcgcgaccg cgggctcggc ctccaagtgc gcccgggtgc tcgagctcgg tgccgaccat
     3961 gccatcgact acaaggccga ggatttcggc gtccgcgcgc tggaattcac cggcggccgc
     4021 ggggtcgacg tcgtcctcga catggtcgcg ggcgactacg tggcgcgcga gctcgcctgc
     4081 ctggccgagg acggtcgcgt cgtgatcatc gcggtgcagg gcggcaccaa ggcgcagatc
     4141 gatgccgggc tcgtgctgcg caagcggctc gtcgtgaccg gctcgacgct gcgcccccgt
     4201 ccggtcgcgt tcaagggcgc gatcgctgcg gcgctgcggg agaaggtgtg gccgctgctc
     4261 gaggcacggc gagtgcagcc ggtgatccag caggtgttcc cggccgccga ggcggcgcgc
     4321 gcgcacgccg cgatggagca gggcgaccac gtgggcaagc tgctgctcgc atggcgctga
     4381 tccccgcaac gacaacgaca aatccaccac catgcgcaag aaactcgtcg ccggcaactg
     4441 gaagatgcac ggctcgcatc cgcagaacgc ggagctgctg gccggcatcc tggcggcgcg
     4501 cccgttcggc tgcgacgtcg cggtctgcgt gccgttcccg tacctgtcgg aggtcgcggt
     4561 ggcgctcgcc ggcagcgaca tccgctgggg cgcgcaggac tgctcgccgc acgcacaggg
     4621 cgcctacacc ggcgaggtct cggccgcgat gctggccgag ttcggctgcc gctacgcgat
     4681 cgtcggccac tccgagcgcc gcgcgatgca cgccgagtcc gaccagctcg tcgccgacaa
     4741 ggccaaggcc gtgctcgggc gcgggctcac gcccatcgtc tgcgtcggcg agacgctggc
     4801 gcagcgcgag cagggcgtcg cggccgaagt ggtcaagcgc cagctctcgg cagtgatcca
     4861 cgcgctcggc cattgcgtcg gcgagatggt cgtcgcctac gagccggtat gggccatcgg
     4921 caccggcaag acggcgtcgc ccgagcaggc gcaggagatg catgcgctga tccgcgccca
     4981 gctgcacgcg gccaccgagc gagccgacgc catgacgctg ctgtacggcg gcagcgtcaa
     5041 gcccgacaac gccgcgacgc tgttcgcgca gcccgacatc gacggcggcc tcatcggcgg
     5101 cgccgcgctg aaggcggccg acttcatcgc catctgccgc gccgccggct gacagcccgg
     5161 caggcacgcc ggcattcgaa atccctggag ctccaaccaa catgcaaatc tggctgaacc
     5221 ttctcttcat cctgcagatc ctggccgcgg tcacgatgat cggcctcgtc ctgctgcagc
     5281 acggcaaggg cgccgacatg ggcgcgtcct tcggcagcgg cgcctcgggc agcctgttcg
     5341 gcgccaccgg cagcgcgaac ttcctctcgc gctcgaccgc catctgcgcg acgatcttct
     5401 tcgccgccac cctggggctc gccggtatcg cgaccaaccg cgtcacggtg cacgcgccga
     5461 ccacctcgtc ggacaacgcg ctgacgcagg cgctgccggc cagcgcggcg gcgacggtcg
     5521 tcgtgcccac cgcctcggcg ccgtccgcgg gcgcctcggt tgccgcgtcc gcggcgacga
     5581 cggcgtctgc gccggccacg ggttcccagg gtgtgccgac gcgctgatcg aacttgcaca
     5641 acagtcggcg caaggcaact gcgagattaa tttttcagta gggaatccga gttaaggctc
     5701 attctttcgc tatagaatgt gaggctgttc agcaagcgat cctcaggcaa gccgaacggt
     5761 tcatccagga cggtcacacg atcgttcagg ccgacgtggt gaaattggta gacacgctat
     5821 cttgaggggg tagtggcgaa agctgtgcga gttcgagtct cgccgtcggc accagttctt
     5881 tctcgctctt tgaaatcccg gaccgccaga tgctccatcg tcggagcgac cggtcccgcg
     5941 ccctgcgtat tcgtccgatg cccgcccgcg cccaccgcgt ggcccgctcg gaaactcacg
     6001 gcgaaacgtg cgggcgcact cgcgatcagc ctgtcggtga tcagtcgagg cgctcttttt
     6061 tctgcccgca tcagacgaac gacgacgccc gcaacgacat cgacaagcca tggacctgac
     6121 cgcctatctc cccgtcatcc tgttcatcct ggtcggggtg ggcgtcggcg tcgcgccgca
     6181 agtgctcggc ttcgtcctcg gccccagccg gccggatgcg cagaagaact ccccgtacga
     6241 gtgcgggttc gaggccttcg aggacgcgcg catgaagttc gacgtgcgct actacctcgt
     6301 cgccatcctc ttcatcctgt tcgacctcga gatcgccttc ctcttcccgt gggcggtcgc
     6361 gctgaaggac atcggccaga ccggcttctg ggccatgatg atcttcctcg cgatcctggt
     6421 cgtcggcttc atctacgaat ggaaaaaggg cgcgctggac tgggagtgat cccgcagcgg
     6481 agcggcagct atggcaaaag gcaatgaagg tctcctgaag gaagggttcg tcaccaccca
     6541 ggtcgacaaa ctcgtcaact ggtccaagac gggctcgctg tggccgatga cgttcggcct
     6601 cgcctgctgc gcggtggaga tgatgcacgc tggcgccgcg cgctacgaca tcgaccgctt
     6661 cggcatgctg ttccgtccga gcccgcgcca gagcgacctg atgatcgtcg ccggcacgct
     6721 gtgcaacaag atggcgccgg cgctgcgcaa ggtctacgac cagatggccg agccgcgctg
     6781 ggtcctgtcg atgggctcgt gcgccaacgg cggcggctac taccactaca gctactccgt
     6841 cgtgcgcggc tgcgatcgca tcgtgccggt ggacgtctac gtgcccggct gcccgccgac
     6901 cgccgaggcg ctgctgtacg ggatcctgca gctgcaggcc aaggtgcggc gagagaacac
     6961 catcgcgcgc gcctgacccc ctcgggtgcg cgaacgtctg gcaggaagac agcacaacaa
     7021 tgacccaacg actcgatgcc ctgcaagccg ccctcgaggc cgcgctcggc gcccgcatcg
     7081 cgcagttcaa gcgcgagcgt ggcgagatct cgatcaccgt caaggcggcg gactcgctcg
     7141 ccgtcgccac gcagctgcgc gacgacccga cgctcggctt cgagcagctg atcgacgtct
     7201 gcggcgtcga cttctcgtcg tacaaggagc agccgtggga cggcccgcgc tactgcgccg
     7261 tcacgcacct gctgtcccac cgccacaatt ggcgcctgcg cctgaaggtc ttcgcgcccg
     7321 acgacgacct gcccgtcgtc ccgtcgctga ccccgatctg gaactcggcc aactggtacg
     7381 agcgcgaggc gttcgacatg tacggcatcc tcttcgaggg ccacgacgac ctgcgccgca
     7441 tcctcaccga ctacggcttc atcggccacc cgatgcgcaa ggatttcccg gtcacgggcg
     7501 atgtcgagat gcgctacgac gccgagcagc gccgcgtgct ctaccagccg gtcacgatcg
     7561 agcagcgcga gatcacgccg cgcgtcgtcc gcgaagacaa ctacggcggc ctgcgctgaa
     7621 cgggcgcgcc cgcgcccgcg ctcccgcccg cctcgttccc tgcatccgac cctgcgccgc
     7681 ttcagaaacg gacagcaagc catggctgaa atcaagaact acacgctcaa cttcgggccg
     7741 cagcaccccg cggcccacgg cgtgctccgc ctcgtgctgg agctcgacgg cgaagtgatc
     7801 cagcgtgccg acccgcacat cggcctgctg caccgcgcga ccgagaagct cgccgagacc
     7861 aagaccttca tccagtcgct gccctacatg gatcgcctcg actacgtgtc gatgatgtcc
     7921 aacgagcagg cctactgcct cgcgatcgaa cgcctgctgg gcatcgacgt gccgctgcgc
     7981 gcgcagtaca tccgcgtgat gttcgcggag atcacgcgcc tgctgaacca cctgatgtgg
     8041 ctcggcgcgc acggcatcga ctgcggcgcg atgaacgtgc tgatctactg cttccgcgag
     8101 cgcgaggacc tgttcgacat gtacgaggcg gtctcgggcg cgcgcatgca tgcggcctac
     8161 ttccgtcctg gcggcgtcta tcgcgacctc cccgacgcga tgccgcagta caaggcctcg
     8221 cgcatccaca acgccaaggc gatcgctcgc atgaacgaga accgccaggg cacgctgctc
     8281 gacttcatcg aggacttcac gaagcgcttc cccaagcacg tcgacgagta cgagacgctg
     8341 ctgaccgaca accgcatctg gaagcagcgc acggtcggca tcggcgtcgt ctcgcccgag
     8401 cgcgcgcgcc agctgggctt caccggcccg atgctgcgcg gctccggcat cgagtgggac
     8461 ctgcgcaaga agcagcccta cgacgtctac gacaagatgg acttcgacgt gcccgtcggc
     8521 gtgaacggcg atacctacga ccggtatctc gtgcgcgtcg aggagatgcg ccaggccaac
     8581 cgcatcatcg cccagtgcgt gaagtggctg aaggccaacc ccggtccggt gatcacggcc
     8641 aaccacaagg tggccccgcc gtcgcgcacc gagatgaagt ccaacatgga agagctgatc
     8701 caccacttca agctcttcac cgaaggcttc cacgtgcccg agggcgagca gtacgcggcc
     8761 gtcgagcacc ccaagggcga gttcggcatc tacctgatca gcgacggcgc caacaagccg
     8821 taccggctga agatccgcgc gcccggcttt gcccacctgg ccgcgatgga cgagatgtcg
     8881 cgcggccaca tgctggccga tgcggtcgcc gtgatcggca ccatggacat cgtgtttggc
     8941 gagatcgatc ga
//
LOCUS       DASVLN010000999         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_213941, whole genome shotgun sequence.
ACCESSION   DASVLN010000999 DASVLN010000000
VERSION     DASVLN010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213941"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VF453_23435"
     CDS             complement(<1..740)
                     /locus_tag="VF453_23435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEX7690676.1"
                     /translation="MPSPSRRLLLALAWLAAALAASALAGAIAFERARASLREDAAVQ
                     AGADALALQSALEKVETLPFVVSLHPLVARGLRSPDDRAQVEALDAYLADVQRQARIA
                     AVYVVEPSGLTIASSNWQTGQSFVGNNYRFRPYMSAALAGRTGRFYGIGATTGEPGYF
                     LGQPVFAADADGVRQVVGVVVIKVDLQEIERAWPPPDDEAVALADAHGVLFLANVPAW
                     RYRSLAPLGETARREIGATQQYAGRDVA"
     gene            complement(812..2176)
                     /locus_tag="VF453_23440"
     CDS             complement(812..2176)
                     /locus_tag="VF453_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS family transporter"
                     /protein_id="HEX7690677.1"
                     /translation="MTTSTSSSPPLSATAAAQATPVPAPGSRAHVIRAIVSASSGNLV
                     EWFDFYAYAFTAVYFAGAFFPKGDPTSQLLNTAGVFALGFFMRPLGSWMFGRIADRRG
                     RRAAMLVSVLMMCFGSLMVACLPTYAQIGVWAPVLLLVARLIQGLSVGGEYGTAATYM
                     SEVAVGGRRGFYGSFQYVTLIGGQLLSTLVLTLLLMVLSPQEMRAWGWRLPFVAGAAA
                     AAIALWLRRSLVETSSAATRARGGAGTLKAMMAHPRALATVIGYTAGGSLLFYTYTTY
                     MQKYLINSAHMDARVATSVMTAALFVFMVLQPAFGALADRIGRRESMICFGLFGVLAT
                     VPLMTSIGHVESPVAAFCLVTLALAGTSFYTSISGLVKAEMFPPHVRALAVGLSYAIG
                     NAVFGGSAEYVALWFKGAGHESAFFWYVVVLAAVALLASWSMPDPRRHGFLRDGTDPA
                     RDAT"
BASE COUNT          377 a          853 c          921 g          272 t
ORIGIN      
        1 ctggcgacgt cgcggccggc gtactgctgc gtggcgccga tctcgcgccg cgcggtctcg
       61 cccagcggcg cgaggctgcg gtagcgccag gccggcacgt tggcgaggaa gagcacgccg
      121 tgcgcatccg cgagcgcgac ggcctcgtcg tcgggcggcg gccaggcgcg ctcgatctcc
      181 tgcaggtcga ccttgatgac gaccacgccg acgacctgcc gcacgccgtc ggcgtccgcc
      241 gcgaacaccg gctggccgag gaagtagccg ggctcgccgg tggtcgcgcc gatgccgtag
      301 aagcggcccg tccggcccgc gagcgcggcg ctcatgtagg gccggaagcg gtagttgttg
      361 ccgacgaagc tctggcccgt ctgccagttg ctcgaggcga tcgtcaggcc cgagggctcg
      421 acgacgtaga ccgccgcgat gcgcgcctgg cgctgcacgt cggcgaggta ggcgtccagc
      481 gcctcgacct gggcgcggtc gtcgggggac cgcagcccgc gcgccaccag cggatgcagc
      541 gagacgacga agggcagcgt ctcgaccttc tccaacgcgg actgcagcgc cagcgcgtcg
      601 gcgccggcct gcacggcggc gtcctcgcgc aggctcgcgc gcgcacgctc gaaggcgatc
      661 gcgccggcca gcgccgaggc ggcgagcgcg gcggcgagcc aggcgagggc caggagcagg
      721 cgacgggagg gcgagggcat cgtgaagcgg cggagcgcga gccccgccac tgtagacccg
      781 cccggccgcg cggcggaggc gatcccgagc attacgtcgc gtcgcgtgcg ggatcggtgc
      841 cgtcgcgcag gaacccgtgg cggcgcgggt cgggcatgga ccacgacgcg aggagcgcga
      901 ccgccgccag cacgaccacg taccagaaga acgccgactc gtggccggcg cccttgaacc
      961 acagcgcgac gtattcggcc gagccgccga agaccgcgtt gccgatcgcg tacgacaggc
     1021 ccacggccag cgcgcgcacg tggggcggga acatctcggc cttcacgagc ccgctgatcg
     1081 aggtgtagaa gctcgtgccc gcgagcgcga gcgtgaccag gcagaacgcc gcgaccgggc
     1141 tctcgacgtg gccgatcgac gtcatcagcg gcacggtggc gaggacgccg aacaggccga
     1201 agcagatcat cgactcgcgc cgcccgatgc ggtcggcgag cgcgccgaac gcgggctgca
     1261 gcaccatgaa gacgaacagc gccgccgtca tcacgctggt ggcgacgcgc gcgtccatgt
     1321 gggcgctgtt gatcaggtac ttctgcatgt acgtggtgta cgtgtagaac agcagcgagc
     1381 cgccggccgt gtagccgatc acggtggcga gcgcgcgcgg atgcgccatc atcgccttga
     1441 gcgtgccggc gccgccgcgc gcgcgggtcg ccgcgctgga ggtctcgacc agcgagcgtc
     1501 gcagccacag cgcgatcgcc gccgccgcgg cgccggccac gaagggcagg cgccagcccc
     1561 aggcgcgcat ctcctgcggg ctgagcacca tcagcagcag cgtcagcacg agcgtggaca
     1621 gcagctgccc gccgatcagc gtcacgtact ggaacgagcc gtagaagccg cgacgcccgc
     1681 cgaccgcgac ctcgctcatg taggtcgcgg cggtgccgta ctcgccgccg accgacaggc
     1741 cctggatcag ccgcgccacc agcagcagca ccggcgccca cacgccgatc tgcgcgtagg
     1801 tcggcaggca ggcgaccatc agcgagccga agcacatcat cagcaccgag accagcatcg
     1861 cggcgcggcg gccgcggcgg tcggcgatgc gtccgaacat ccagctgccc agcggccgca
     1921 tgaagaagcc gagcgcgaac acgccggcgg tgttgagcag ctgcgaggtg ggatcgccct
     1981 tggggaagaa cgcgcccgcg aaatagaccg ccgtgaacgc ataggcgtag aagtcgaacc
     2041 actcgaccag gttgccggac gaggcgctca cgatggcgcg gatcacgtgg gcgcggctgc
     2101 cgggcgccgg gacgggggtg gcctgggccg ccgcggtggc ggacaggggc gggctgctcg
     2161 aggtcgaggt ggtcatgctt gtctcctggg tcgtcgtgga tgtcgcggct ccgcgcggga
     2221 gcctgccgtc cggtatcgca acgcccgtgc cagcgcggcg acgtgcgcaa gtccttgatt
     2281 cgccggggcg cggaggcggt gcacgtccgg cggtccggaa tcgcgggcgc gacggatccg
     2341 gccggccgga ctggcggcgc gcggcggggc gcgcgtaaac tccggcgcgc acatgacgac
     2401 cccaccgaat ccgccgaagg cgc
//
LOCUS       DASVLN010001000         5303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_50739, whole genome shotgun sequence.
ACCESSION   DASVLN010001000 DASVLN010000000
VERSION     DASVLN010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50739"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VF453_23445"
     CDS             complement(<1..511)
                     /locus_tag="VF453_23445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7690678.1"
                     /translation="MHALLVVAHPSPDSFSHALAHAAREALVEREFAVDSHDLYAEGF
                     DPVQRTGEAGNTASADALVERHCAELARADLVVVCHPNWWGQPPAILKGWIDRVFRLG
                     TAYGYPPGVPPEGVPTGLLRAREALVFNTSNTPAAREAEAFGDPLDALWRRCVFALCG
                     VARVTRRTAG"
     gene            complement(586..729)
                     /locus_tag="VF453_23450"
     CDS             complement(586..729)
                     /locus_tag="VF453_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690679.1"
                     /translation="MDTTLQQAAAERQHDLEAAHRGTELACDGAMRTFKDAAQAMRDA
                     AGY"
     gene            942..3275
                     /gene="parC"
                     /locus_tag="VF453_23455"
     CDS             942..3275
                     /gene="parC"
                     /locus_tag="VF453_23455"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX7690680.1"
                     /translation="MEQTPLAFDGGGDGSDALTLAHYAERAYLEYALSVVKGRALPDV
                     SDGCKPVQRRILYAMHLKGLSFTDASRSGPSPRKSAAVVGDVLGMFHPHGDTAAYDAL
                     VRMAQDFSLRYPLIDGQGNFGSRDGDGAAAMRYTEARLNPIARLLLDEIDEGTVDFMP
                     NYDGTTTEPRLLPARLPFVLLNGASGIAVGLATEVPSHNLREVANAAIALIKDEHLGD
                     DALLAEIPGPDYPGGGQIISSPADIREAYASGRGSLKVRARWKIEDLARGQWQMVVTE
                     LPPGVSARVVLEEIEELTNPKVKAGKKALSAEQLQLKATVLGVLDGQRDESNKDAPVR
                     LVFEPKSRTVDPQELANTLLAHTSLETSAPINLTMVGGDGRPTQKSLRQVLSEWVAFR
                     LHTVRRRTEHRLAHVLDRIHILEGRQLVLLNIDEVIRIIRESDEPKPALIERFGLSDR
                     QAEDILEIRLRQLARLEAIRIEQQLAEYREQQKKLEDILGSPAVLKRTVIKEIEADAK
                     AHGDERRTLIQADKRAVAEVKVVDEPVTVIVSLKGWVRAQKGHGHDAAGFAFKSGDAL
                     YGTFECRTVDTLLVFGSNRGGTGRVYTVAVGALPGARGDGAPITTLIELESGTQPLHY
                     FAASAEATLLLANTGGFGLLAKVGDMTSRQKGGKSFLSIADEDRLLPPVPVLAGQAQV
                     ACLSMGGRLLVFPLDELKVQPNGGKGLTLMDVDAKDPLVSVATFANALRVLGTGRGGK
                     PREEDLKGAALAAHVGKRARKGKAIEAAIKGLRVLAI"
     gene            3472..4743
                     /locus_tag="VF453_23460"
     CDS             3472..4743
                     /locus_tag="VF453_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7690681.1"
                     /translation="MHLKLTTLSTFAVPAIAVAVASVFAVPAQAGEVEVLHYWTSPGE
                     AKAAAALKQTLQQQGDTWKDFAVAGGGGDAAMTVLKSRVISGNPPSAAQIKGPAIIEW
                     AEQGVLENMDAVAGPGQWDSLIPKVVADGLKYQGHYVAVPVNVHRVNWLWTNPEAFRK
                     AGAKVPATWDEFFVAAEALKKAGITPVAHGGQNWQEFELFETVALGVGGADFYRKAFV
                     RLDPAALKSPTMEKSLATFKRIHDYVDRNAPGRDWNLATAMVIRGDAAMQFMGDWAKG
                     EFLAAGKVPGKDFGCAPAPGTANAYTYNVDSFAMFKVKDPANQKAQRDLATAIMSPAF
                     QEVFNLNKGSIPVRAGVSLAKFDECAKRSAADFAATTKNGGLVPSIAHDMAVSPAIEG
                     AMKDAISAFWNDEKGTPAAVMDRLVAAAKVK"
     gene            complement(4781..>5303)
                     /locus_tag="VF453_23465"
     CDS             complement(4781..>5303)
                     /locus_tag="VF453_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690682.1"
                     /translation="GGGVAQAGAIEGVDPAAVQVVNTGTGGDKTFNVTNNTKQEESFT
                     YSVQGQNKGTITLQPGQVGAFTAGSGDIGVRITPTDRNGNAHPDEVLYEDGGAGNGQA
                     RGAGNPDISKVDGNKDFSGKAMNMTVTMSDGGKAGDGDPIHAYAYSTDDAASMGLAGN
                     TSKTANIVMSDAT"
BASE COUNT          785 a         1862 c         1930 g          726 t
ORIGIN      
        1 ggcccgccgt gcgccgcgtg acgcgcgcga cgccgcacaa cgcgaagacg cagcgccgcc
       61 agagcgcatc cagcgggtcg ccgaaggcct cggcctcgcg cgcggcgggc gtgttggagg
      121 tgttgaagac gagcgcctcg cgtgcccgca gcaggcccgt cggcacgccc tcgggcggca
      181 ccccgggcgg gtacccgtag gccgtgccga gccggaagac gcggtcgatc cagcccttca
      241 ggatcgccgg cggctggccc caccagttgg gatggcagac gacgacgagg tcggcgcgcg
      301 cgagctccgc gcagtggcgc tcgacgagcg cgtccgccga cgcggtgttg ccggcctcgc
      361 cggtgcgctg cacggggtcg aagccctcgg cgtagaggtc gtgcgagtcg acggcgaact
      421 cgcgttccac gagcgcctcg cgcgcggcgt gcgcgagggc gtggctgaag ctgtccggcg
      481 acggatgggc gacgacgagg agcgcgtgca tgggcgatgg ccaggggcga cgggtcgcca
      541 gcttagccga tgggcgagcg cgtcggcccg gccgcccccg gggcttcagt agccggccgc
      601 gtcgcgcatc gcctgcgccg catccttgaa ggtgcgcatc gcgccgtcgc aggcgagctc
      661 cgtgccgcgg tgcgcggcct ccaggtcgtg ctgccgctcg gcggcggcct gctgcagggt
      721 cgtgtccatg gcggggctcc ttggttctcg tggatgggac gcgcgacggc gtgcgccggg
      781 ccacgggcaa tcggtgcgcc gctcgcccag ccgcgatact cggggctttt tcgcgcgcgc
      841 cgcgccctcg tccgaggcag ggccgccgga atgtcgtatc gtgcacgccg ccgcgcgtcc
      901 gtcaccgggc gcggcctcac gaaggatccc cgagcttgac catggaacag acccccctcg
      961 cgttcgacgg cggcggcgac ggcagcgacg cgctgaccct cgcgcactat gccgagcgcg
     1021 cctacctcga atacgcgctg agcgtcgtca agggccgcgc cctgcctgac gtctcggacg
     1081 gctgcaagcc cgtgcagcgc cgcatcctgt acgcgatgca cctgaagggc ctgtccttca
     1141 ccgacgcgag ccgctcgggc ccgtcgccgc gcaagagcgc cgcggtcgtc ggcgacgtgc
     1201 tgggcatgtt ccacccgcac ggcgacaccg cggcctacga cgcgctcgtg cgcatggcgc
     1261 aggacttcag cctgcgctac ccgctgatcg acggccaggg caacttcggc agccgcgacg
     1321 gcgacggcgc cgcggcgatg cgatacaccg aggcgcgcct gaacccgatc gcgcgcctgc
     1381 tgctcgacga gatcgacgag ggcaccgtcg acttcatgcc gaactacgac ggcacgacga
     1441 ccgagccgcg cctgctgccc gcgcgcctgc ccttcgtgct gctcaatggc gcgagcggca
     1501 tcgcggtcgg cctcgccacc gaggtgccca gccacaacct gcgcgaggtc gccaacgccg
     1561 cgatcgcgct gatcaaggac gagcacctgg gcgacgacgc gctgctcgcg gagatccccg
     1621 gcccggacta tcccggcggc ggccagatca tcagcagccc ggccgacatc cgcgaggcct
     1681 atgcgagcgg ccgcggcagc ctgaaggtgc gcgcgcgctg gaagatcgag gatctcgcgc
     1741 gcggccagtg gcagatggtc gtcaccgagc tgccgccggg cgtgagcgcg cgcgtcgtgc
     1801 tcgaggagat cgaggagctc accaacccca aggtcaaggc cggcaagaag gcgctgagcg
     1861 ccgagcagct gcagctgaag gcgacggtgc tgggcgtgct ggacggccag cgcgacgagt
     1921 ccaacaagga cgcgcccgtg cgcctggtct tcgagcccaa gagccgcacc gtcgaccccc
     1981 aggagctcgc caacacgctg ctcgcccaca ccagcctcga gacctccgcg cccatcaacc
     2041 tcacgatggt cggcggcgac ggccgcccga cgcagaagag cctgcgccag gtgctgtcgg
     2101 agtgggtcgc cttccgcctg cacacggtcc ggcgtcgcac cgagcaccgc ctcgcgcacg
     2161 tgctcgatcg catccacatc ctcgagggcc ggcagctggt gctgctgaac atcgacgagg
     2221 tgatccgcat catccgcgag agcgacgagc ccaagcccgc gctgatcgag cgcttcggcc
     2281 tcagcgaccg ccaggcggag gacatcctcg agatccggct gcgccagctg gcgcggctcg
     2341 aggcgatcag gatcgagcag cagctcgccg agtaccgcga gcagcagaag aagctcgagg
     2401 acatcctcgg cagccccgcc gtcctcaagc gcacggtgat caaggagatc gaggcggacg
     2461 ccaaggccca cggcgacgag cgccgcacgc tgatccaggc cgacaagcgc gccgtcgccg
     2521 aggtcaaggt ggtcgacgag ccggtgacgg tcatcgtgag cctgaagggc tgggtgcggg
     2581 cgcagaaggg ccacggccac gacgccgcgg gcttcgcctt caagtcgggc gatgcgctgt
     2641 acggcacctt cgaatgccgc accgtggaca cgctgctcgt cttcggcagc aaccggggcg
     2701 gcacgggacg ggtctatacc gtggcggtgg gcgcgctgcc gggcgcccgt ggcgacggcg
     2761 cgccgatcac gacgctgatc gagctcgagt ccggcaccca gccgctgcac tacttcgcgg
     2821 ccagcgcgga ggcgaccctg ttgctggcca acacgggcgg cttcggcctg ctcgccaagg
     2881 tgggcgacat gacgagccgc cagaagggcg gcaagagctt cctgtccatc gccgacgagg
     2941 atcgcctgct gccgccggtg ccggtgctgg cggggcaggc gcaggtcgcg tgcctgagca
     3001 tgggcggccg cctgctcgtc ttcccgctcg acgagctgaa agtccagccc aacggcggca
     3061 agggcctcac gctgatggac gtcgacgcga aggatccgct ggtctcggtc gcgaccttcg
     3121 cgaacgcgct gcgggtgctg ggcacgggcc gcggcggcaa gcccagggag gaggacctca
     3181 agggcgccgc gctcgcagcg cacgtcggca agcgggcgcg caagggcaag gcgatcgagg
     3241 cggcgatcaa gggcctgcgc gtgctggcga tctgatcgcc gcgcgcccag ggacgacgcc
     3301 cggcgtgccc gggcgttctt cattgcgcac ggtccgtcgt gccggtgcag ccggggggcg
     3361 accgggtcct tcgctcgcac cccccgcgac ggggcgcctc gtccgggaaa ctaccgtgct
     3421 gcctgcgcgc cgctcgccgt agattcctgg acttcacatt cggagccccg catgcacctg
     3481 aaattgacaa cgctgtctac gtttgccgtc cccgccatcg ccgtcgcggt ggcctccgtg
     3541 ttcgccgtgc cggcgcaggc aggcgaggtc gaggtcctgc actactggac gtccccgggc
     3601 gaggcgaagg cggccgcggc gctcaagcag acgctgcagc agcagggcga cacgtggaag
     3661 gacttcgcgg tcgccggtgg cggcggcgac gcggcgatga cggtgctgaa gtcgcgcgtg
     3721 atctccggca acccgccgtc ggcggcgcag atcaagggcc cggcgatcat cgagtgggcc
     3781 gagcagggcg tgctggagaa catggacgcc gtcgccgggc cggggcagtg ggactcgctg
     3841 atccccaagg tggtcgccga cggcctgaag taccagggcc actacgtcgc cgtgccggtg
     3901 aacgtgcatc gcgtgaactg gctgtggacg aaccccgagg ccttccgcaa ggccggcgca
     3961 aaggtgcccg cgacctggga cgagttcttc gtcgccgccg aggcgctgaa gaaggccggc
     4021 atcacgccgg tggcccacgg cggccagaac tggcaggaat tcgagttgtt cgagacggtg
     4081 gccctgggcg tcggcggggc ggacttctac cgcaaggcgt tcgtgcggct cgatcccgcg
     4141 gcgctcaaga gcccgacgat ggagaagtcg ctcgccacgt tcaagcgcat ccacgactac
     4201 gtcgaccgca acgcgcccgg ccgcgactgg aacctcgcca cggcgatggt gatccgcggc
     4261 gacgccgcga tgcagttcat gggcgactgg gccaagggcg agttcctggc cgccggcaag
     4321 gtgcccggca aggacttcgg ctgcgcgccc gcgcccggca cggccaacgc gtacacctac
     4381 aacgtcgaca gcttcgcgat gttcaaggtc aaggatccgg ccaaccagaa ggcgcagcgc
     4441 gacctcgcga cggcgatcat gtcgcccgcg ttccaggaag tcttcaacct gaacaagggc
     4501 tcgatcccgg tgcgcgcggg cgtgtcgctc gcgaagttcg acgaatgcgc gaagcgctcg
     4561 gccgcggact tcgccgcgac gaccaagaac ggcggcctcg tgccctcgat cgcccacgac
     4621 atggccgtct cgccggccat cgaaggcgcg atgaaggacg cgatctccgc cttctggaac
     4681 gacgagaagg gcacgcccgc ggcggtgatg gatcggctcg tggcggcggc gaaggtgaag
     4741 tgacgcatgg cgggggcgcg ggcgtcgcgc gccggtcgcg tcaggtcgcg tcggacatca
     4801 cgatgttggc ggtcttgctc gtgttgccgg ccaggcccat cgaggcggcg tcgtcggtcg
     4861 agtacgcgta ggcgtggatg ggatcgccgt cgccggcctt gccgccgtcg gacatcgtca
     4921 cggtcatgtt catcgccttg ccggagaagt ccttgttgcc gtcgaccttg ctgatgtcgg
     4981 gattgccggc gcccctggcc tggccgttgc cggcgccgcc gtcctcgtag agcacctcgt
     5041 cggggtgggc gttgccgttg cggtcggtgg gcgtgatgcg cacgccgatg tcgcccgagc
     5101 cggccgtgaa ggcgccgacc tggccgggct ggagcgtgat cgtgcccttg ttctgcccct
     5161 gcacggagta ggtgaacgac tcctcctgct tcgtgttgtt cgtgacgttg aaggtcttgt
     5221 cgccgccggt gccggtattg acgacctgca ccgcggcggg atccacgcct tcgatggccc
     5281 cggcctgcgc cacgccgccg ccg
//
LOCUS       DASVLN010001001         3884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_375555, whole genome shotgun sequence.
ACCESSION   DASVLN010001001 DASVLN010000000
VERSION     DASVLN010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375555"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..636
                     /locus_tag="VF453_23470"
     CDS             412..636
                     /locus_tag="VF453_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEX7690683.1"
                     /translation="MMEFLVTGMSCGHCASVIREAVTALDPAAVVDVDLGTSRVRIDS
                     ELDESLLSQAIVDAGYAPTPVMNFTAETRS"
     gene            complement(643..1059)
                     /locus_tag="VF453_23475"
     CDS             complement(643..1059)
                     /locus_tag="VF453_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7690684.1"
                     /translation="MSDDTPSARERLASCCCGQLRVRTRGEPVRVSMCHCLECQRRCG
                     SVFAAQARFPDDRVEIEGESTEYSRTGDEGGVARFHFCPRCGSTVWYVFDGAPGMTVV
                     TVGAFADPHFPPPSFSVYEDRRHAWVAVPEGAERWA"
     gene            complement(1069..1371)
                     /locus_tag="VF453_23480"
     CDS             complement(1069..1371)
                     /locus_tag="VF453_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690685.1"
                     /translation="MASKRPRLPPMTPYRNRSGRSGVVAYALVRDAILLEFAQGEVYE
                     YTARSAGPGVIAEMRSRAERGSGLATYVSRHVRGAYARHFEAGPRERPDSPRARPA"
     gene            complement(1571..1765)
                     /locus_tag="VF453_23485"
     CDS             complement(1571..1765)
                     /locus_tag="VF453_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX7690686.1"
                     /translation="MNKDQVNGRMETAKGNVKEAAGKVVGNDRLAAEGRADQAAGKVQ
                     STAGDLKNKAGNAIKNAIDR"
     gene            complement(2018..2446)
                     /locus_tag="VF453_23490"
     CDS             complement(2018..2446)
                     /locus_tag="VF453_23490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690687.1"
                     /translation="MNEVLHEVVAAASAAVATLQERAGGRLDYSPASLAAVDDILAEV
                     SGYVSELDEGVVTGLVQQLGCYVLEVARRAYGGSYYWHEEGEQPVLVVGEPEAHVALM
                     TWSKVTGRVTGDEGDAIPFFFEGFAERAAAPEPGSSVLFL"
     gene            complement(2507..2761)
                     /locus_tag="VF453_23495"
     CDS             complement(2507..2761)
                     /locus_tag="VF453_23495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690688.1"
                     /translation="MKFISLRVRTGLVMHGYTADNQEIVDELHDEPFVEKLIAVDRIQ
                     SVTEKYLLVTSSHGRVLYWEYEGSLADVTARLAAAGLVVA"
     gene            complement(2781..3626)
                     /locus_tag="VF453_23500"
     CDS             complement(2781..3626)
                     /locus_tag="VF453_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7690689.1"
                     /translation="MRIEIQPGIRLFVDVEGPQFVPDGPMLREKPTLLLLHGGPGFDH
                     SGFKPFFTRLADIAQVVYYDHRGHGRSDRRPPQEWTLDTFADDVVRLCDALGIVKPIV
                     LGQSFGGFVAQRYIDRHPAHPAKVILSSTSPHKKLERKVEMFARLGGPQAGESARRFW
                     TEPNARTWADYGVHCRHLYNTTPQDPQAGARTIHHFDILYASAGGEQRTMDLLPGLAK
                     AQCPVLVMVGEDDPVCPVADALDIAAALPPRWMQLARFADVGHGAWRDDADAAFAVLR
                     RFILE"
     gene            complement(3630..>3884)
                     /locus_tag="VF453_23505"
     CDS             complement(3630..>3884)
                     /locus_tag="VF453_23505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HEX7690690.1"
                     /translation="GSIPVHAPHAGVVAYLRRPGDDVRAGERLADLVEPMSGLVTPLL
                     SPVDGVFYAHDTRRFLPAHASVLRVAGRAAVRTGKLLSAR"
BASE COUNT          550 a         1370 c         1422 g          542 t
ORIGIN      
        1 ggcgacgccc ggcacggcgc gcagcgcccg ctcgacccgc ccggcgcacg cggcgcagct
       61 catgccctcg acggggaacg accattcctg gcgatcgggc aattcggtgg cgagaggcgt
      121 ggtggacatg gcaggcgagg cgggccgggc gccggggcgc tcgtcgtacg tggggtcgat
      181 ggaaggatag gcgagagtgt ccgcgcttgc cgtgcccggc cgcctggcgc gcgtcaaagt
      241 ccacgcgcgc agggctattt gaggacgccg cgccgcactt ccaggccgct tacctcccgc
      301 ggcggcacgt ccaatgcccg ttcgcgggac agggccgccg ccgatccccg agccgtccgt
      361 cacgggccac ggcgacactg gaaccttccc cgccaactcg aaagaaccgc catgatggaa
      421 ttcctcgtca ccgggatgag ctgcggccac tgtgccagcg tcatccgcga agccgtcacc
      481 gccctcgacc cggccgccgt cgtcgacgtc gacctgggca ccagtcgcgt gcgcatcgac
      541 tccgagctcg acgagagcct gttgtcgcag gccatcgtcg acgccggcta cgcgccgacg
      601 ccggtcatga acttcacggc cgagacgcgc agctgagcgg gctcaagccc agcgctccgc
      661 cccctcgggc acggcgaccc aggcgtgccg gcggtcttcg tagaccgaga acgagggcgg
      721 cgggaaatgc gggtcggcga acgcgccgac ggtgacgacc gtcatgcccg gtgcgccgtc
      781 gaagacgtac cacaccgtgc tgccgcagcg cgggcagaag tggaagcgcg cgacgccgcc
      841 ctcgtcgccg gtgcgcgagt actccgtcga ctcgccctcg atctcgaccc ggtcgtccgg
      901 gaagcgcgcc tgcgccgcga agacgctgcc gcagcggcgc tggcattcga ggcagtggca
      961 catcgacacg cgcaccggct cgccgcgcgt gcgcacgcgc aactggccgc agcagcagga
     1021 agcgaggcgt tcgcgggcgg agggggtgtc gtcgctcatc gcaacagtct aggcgggccg
     1081 cgcccgcggc gagtccggcc tttcccgcgg gccggcctcg aagtgccgtg cataggcgcc
     1141 gcgcacgtgc cgcgagacgt aggtcgcgag cccgctgccg cgctcggcgc gcgaacgcat
     1201 ctcggcgatc acccccggcc cggcgctgcg cgcggtgtac tcgtagactt cgccctgcgc
     1261 gaactccagc aggatcgcgt cacgcacgag cgcataggcg acgacgcccg agcgcccgct
     1321 gcggttgcgg tagggcgtca tcggcggcaa ccgggggcgc ttggaggcca tgcccggaag
     1381 cggcaagagc gatgccgtgc gccggcctgc gagaccgatg cggcgcccag gaagccatgg
     1441 gcgccttctc ggggggcatc gctgccccac gaagacaagc gcccggacgt gcactggacg
     1501 tccgggcgct ccggggaagg cgccagccgc ggggagagga tccgcggaag gcgccggcag
     1561 cggcgcggga tcagcggtcg atcgcgttct tgatcgcgtt gccggccttg ttcttgaggt
     1621 cgccggcggt ggactgcacc ttgcccgcgg cctggtcggc gcggccttcg gcggccagcc
     1681 ggtcattgcc gacgaccttg ccggcggctt ccttgacatt gcccttggcc gtttccatgc
     1741 gtccgttgac ttggtccttg ttcatggcgt gctcctttgc ggtgaagtgg acgccgatcg
     1801 acctcgcggt gttgttcggg ttctgtgccc gatgtcgttc ggcttggaat ccactgtgcc
     1861 acccgtccgc ggccggacgt gtcggcctcg cgtcgatcga tgtgtaggac aaggcgcggg
     1921 cgtcgcgcag gccttcgacg acgcccgcgc gcggccgagc gtggaacacg catgcgcggg
     1981 cgttccgagc atcgcatgcc gcacggtacg cggtcgctca gaggaagagc acgctgctgc
     2041 cgggctccgg cgcggcggcg cgctcggcga atccctcgaa gaagaacggg atcgcgtcgc
     2101 cctcgtcgcc cgtgacgcgg ccggtgacct tgctccaggt catcagcgcg acatgggcct
     2161 cgggctcgcc caccaccagc accggctgct cgccctcctc gtgccagtag tacgagccgc
     2221 cgtatgcgcg ccgcgcgacc tcgagcacgt agcagcccag ctgctgcacg agcccggtga
     2281 cgacgccctc gtcgagctcg gagacgtagc ccgagacctc ggccaggatg tcgtcgacgg
     2341 ccgcgaggct cgcgggcgag tagtcgagcc gcccgcccgc gcgctcctgc agcgtggcga
     2401 cggcggccga cgcggcggcg acgacttcgt ggaggacttc gttcatgggc gggctccgcg
     2461 cggcgagggg cacgcgcgtt ctcgcccggt cgccggcctc atcgcgtcag gccaccacga
     2521 gccccgcggc ggccagccgc gcggtcacgt cggccagcga gccttcgtat tcccagtaca
     2581 gcacgcggcc gtgcgaggac gtgacgagca ggtatttctc cgtgaccgac tggatgcggt
     2641 cgaccgcgat cagcttctcg acgaagggct cgtcgtggag ctcgtcgacg atctcctggt
     2701 tgtcggccgt gtagccgtgc atgaccaggc cggtgcgcac gcgcagcgag atgaatttca
     2761 tgggcgtatg gtagcgggcg tcattcgagg atgaagcggc gcagcaccgc gaaggccgcg
     2821 tcggcgtcgt cgcgccaggc gccgtggccg acgtcggcga agcgcgccag ctgcatccag
     2881 cgcggcggca acgcggcggc gatgtcgagg gcgtcggcca ccgggcagac cggatcgtcc
     2941 tcgcccacca tcacgagcac cgggcactgc gccttcgcga ggcccggcag caggtccatc
     3001 gtgcgctgct cgccgccggc cgaggcgtag aggatgtcga agtggtggat cgtgcgcgcg
     3061 ccggcctgcg gatcctgcgg cgtcgtgttg tacaggtggc ggcaatggac gccgtagtcg
     3121 gcccatgtcc gggcgttcgg ctccgtccag aacctgcgcg ccgactcgcc ggcctgcggc
     3181 ccgcccagcc tcgcgaacat ctcgaccttg cgctcgagct tcttgtgcgg cgacgtgctc
     3241 gagaggatca ccttggccgg atgcgccgga tggcggtcga tgtagcgctg cgcgacgaag
     3301 ccgccgaacg actgccccag cacgatcggc ttgacgatgc ccagcgcgtc gcacaggcgc
     3361 acgacatcgt ccgcgaaggt gtcgagcgtc cactcctgcg gcgggcggcg gtcgctgcgg
     3421 ccgtggccgc gatggtcgta gtagacgacc tgggcgatgt cggccaggcg cgtgaagaac
     3481 ggcttgaagc ccgagtggtc gaagccggga ccgccatgca gcagcagcag ggtcggcttc
     3541 tcgcgcagca tcgggccgtc gggcacgaac tgcgggccct cgacgtcgac gaacaggcgg
     3601 atgccgggct ggatctcgat gcgcatggct caacgggcgg acagcagctt gcccgtgcgc
     3661 acggccgcgc gaccggccac gcgcagcacg ctcgcgtgcg cgggcaggaa gcggcgcgtg
     3721 tcgtgcgcgt agaagacgcc gtcgaccggg ctcagcagcg gcgtgaccag gcccgacatc
     3781 ggctcgacga ggtcggcgag gcgctcgccc gcgcgcacgt cgtcgccggg ccggcgcagg
     3841 tacgcgacga cgccggcgtg cggcgcatgc accgggatcg atcc
//
LOCUS       DASVLN010001002         5203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_214135, whole genome shotgun sequence.
ACCESSION   DASVLN010001002 DASVLN010000000
VERSION     DASVLN010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214135"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..2271)
                     /locus_tag="VF453_23510"
     CDS             complement(112..2271)
                     /locus_tag="VF453_23510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX7690691.1"
                     /translation="MTKQSSLRPLVLAAAVATAFGAAAQGTTTTTTTETTRVTTTTQR
                     PDGSLATSTRQAAAAATTTTAPPPGVLGSGIDRSGFDAAVRPQDDLFRATNGAWMAHT
                     EIPADKSAWGTFYTLRDRSDADVRTLAEAAMRSSATAGSDERKIGDFYAAFLDTAAID
                     KAGLAPVEPSLADVDSVRDARRLVELMGHWQGVVPMPIYVDTDPGEDDPAIYAASFKQ
                     SGLGLPDRDYYLKDDPGHAAARRAYLAYATKLLALSGDRQAAAHARAVVALEARIAQA
                     QWPRDQIRDPKRTHNPTAQDALATLAPGADWPAFLAAAQVPARAKAVDVKEPSYVTAL
                     AGLLRSQPLPTWKLYMKVRRLDAAAPVLPHAFRDAHYQYHGVAITGLKQEPARWQQAV
                     DALNAALGQAVGRIYVGEHFPPASKARMLELVHNLLRTYASLIDGLTWMSPETKAAAH
                     AKLDKYGVKIGYPDKWIDYAALDIRPGHALADVDRGAEFEYRRHIVRVGGPIDPTDWE
                     MTPQTVNAYYSPNRNEIVFPAAILQPPFFDMHADDAANYGAIGAIIGHEISHGFDDQG
                     SQYDGDGRLRNWWTDADRAAFKAITSRLVAQYAAYEPLPGQHVNGQLTLGENIADLSG
                     LQIAYKAWKLSLHGQPSPVIDGIDGEQRFFYGFAQAWRTKVRDERQLQLLSIDPHSPG
                     QFRADGAAINADGFHEAFRTKPGDGMWKAPADRIRLW"
     gene            complement(2361..4412)
                     /locus_tag="VF453_23515"
     CDS             complement(2361..4412)
                     /locus_tag="VF453_23515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX7690692.1"
                     /translation="MRLATLVAAVSLACATNAFAADAAVSPMTPVGATAVPGGLDRAG
                     MDPAVRPQDDLFAAMNGHWLQQTQIPADKSEFGTFIELRDLSDERVKGLVEKLAAAPQ
                     AAGSNEARIADYYAGYLDTAAIDKAGLAPLKPWTDQLDAVATRAELVELLGRWQAYVD
                     VPLALQVQPDAKDPHVYAPQAAQGGLGLPDRDYYLKKDAAFAKARAAYAVYVRQLLAL
                     DGDPQAAAHAAAVIALERKLAAAQWSQEALRDPVKAYNPMTLAQLSARAPGVDWKAFV
                     DAGETPVPATLTVLQPAYATAFARLVEREPLETWKQYLRVRLLDARAHVLPAGFREAS
                     FAFHGAAIQGLKEDRARWQQGVDSVNGALGEAVGQLYVAQYFPPAHKARMLELVNNLL
                     AAYAGSIDGLTWMSPQTKAAAHEKLAKYAVKIGYPDHWRDYSALEIRAGDPFGNAMRA
                     SRFDYLRQAVRSGKPVDRGEWDLLPQTVNADYDPNKNAITFPAAILQPPFFDMQADDA
                     VNYGAIGAIIGHEISHGFDDEGSQYDGDGRLRNWWTAADRRAFAGITARLVAQFAAYQ
                     PLPGQHLNGKLTLGENIADLSGLQIAYKAYKLSLGGKEPAPVDGLSGEQRFYLGWSQA
                     WREKVRDDRALQLVTIDPHSPTRFRADGAAMNADGFHEAFGTKPGDRMWKAPADRIRL
                     W"
     gene            complement(4790..>5203)
                     /locus_tag="VF453_23520"
     CDS             complement(4790..>5203)
                     /locus_tag="VF453_23520"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEX7690693.1"
                     /translation="IGARAGQVAHGREVVLHADGVPVVYARSVLQTVHARGTWKAIRG
                     LGTRALADMLFGLPAATRTPFEFVRFAPSSRWAAKVHRRWRDATGAAWGRREVWARCS
                     VFDRRGAKLLVTECFAPAILAYDVPQRARAVAWRR"
BASE COUNT          654 a         1835 c         1963 g          751 t
ORIGIN      
        1 cgggcagcct cgcgatgtcg gcgctcggcc tcgtcgcgat cgccggcctc gcgcgccgcc
       61 gcaagcccgc ggcgcgctga acgcgggccc gcgcgtcgcg ggccatgcgt ctcaccacag
      121 gcggatgcga tccgcgggcg ccttccacat gccgtcgccg ggcttcgtgc ggaaggcctc
      181 gtggaagccg tcggcgttga tggcggcgcc gtctgcgcgg aactggcccg gcgagtgcgg
      241 gtcgatcgac agcagctgca gctggcgttc gtcgcgcacc ttggttcgcc aggcctgcgc
      301 gaagccgtag aagaagcgct gctcgccgtc gatgccgtcg atgaccggcg acggctggcc
      361 gtggagcgac agcttccagg ccttgtaggc gatctgcagc cccgacaggt cggcgatgtt
      421 ctcgcccagc gtgagctgtc cgttgacgtg ctggcccggc aacggctcgt aggcggcgta
      481 ctgcgccacc agccggctcg tgatcgcctt gaaggccgcg cggtcggcgt ccgtccacca
      541 gttgcgcagg cggccgtcgc cgtcgtactg gctgccctgg tcgtcgaagc cgtggctgat
      601 ctcgtggccg atgatcgcgc cgatcgcgcc gtagttcgcc gcgtcgtcgg cgtgcatgtc
      661 gaagaacggc ggctgcagga tcgcggccgg gaacacgatc tcgttgcggt tcgggctgta
      721 gtacgcgttg accgtctgcg gcgtcatctc ccagtcggtc gggtcgatcg ggccgccgac
      781 gcgcacgatg tggcgccggt actcgaactc ggcgccgcgg tcgacgtcgg ccagcgcgtg
      841 gcccgggcgg atgtcgagcg ccgcatagtc gatccacttg tcgggatagc cgatcttgac
      901 gccgtacttg tccagcttgg cgtgcgcggc ggccttggtc tcgggcgaca tccacgtcag
      961 cccgtcgatc aagctcgcgt aggtgcgcag caggttgtgc acgagctcca gcatgcgcgc
     1021 cttcgacgcg ggcgggaagt gctcgccgac gtagatccgg ccgaccgcct ggccaagcgc
     1081 cgcgttcagg gcgtcgacgg cctgctgcca gcgcgccggc tcctgcttca ggcccgtgat
     1141 cgccacgccg tggtactggt agtgcgcgtc ccggaacgcg tgcggcaaca ccggcgcggc
     1201 ggcatccagc cggcgcacct tcatgtacag cttccaggtc ggcagcggct gcgagcgcag
     1261 caggcccgcg agcgccgtga cgtagctcgg ctccttgacg tcgaccgcct tcgcgcgcgc
     1321 cggcacctgc gccgccgcga ggaaggccgg ccagtccgcg ccgggcgcca gcgtcgcgag
     1381 tgcgtcctgc gccgtcgggt tgtgcgtgcg cttcgggtcg cggatctggt cgcgcggcca
     1441 ctgcgcctgc gcgatccggg cctcgagcgc gaccaccgcg cgagcatgcg ccgcggcctg
     1501 gcgatcgccc gacagcgcga gcagcttcgt cgcgtaggcc aggtaggccc ggcgcgcggc
     1561 ggcgtggccc gggtcgtcct tcaggtagta gtcgcggtcg ggcaggccca ggccgctctg
     1621 cttgaacgac gcggcgtaga tggccggatc gtcctcgcca ggatcggtgt cgacgtagat
     1681 cggcatgggc accacgccct gccaatgtcc catgagctcg acgaggcgcc gggcgtcgcg
     1741 caccgaatcg acgtcggcga gggagggctc gaccggcgcc aggccggcct tgtcgatcgc
     1801 ggcggtgtcg aggaaggcgg catagaagtc gccgatcttg cgctcgtcgc tgccggccgt
     1861 cgcggacgaa cgcatcgccg cctcggcgag cgtgcgcacg tccgcgtcgc tgcggtcgcg
     1921 cagcgtgtag aaggtgcccc aggcggactt gtcggccggg atctccgtgt gcgccatcca
     1981 ggccccgttc gtcgcccgga agagatcgtc ctgcggccga accgcggcgt cgaagccgct
     2041 gcggtcgatg ccgctgccca gcacgcccgg cggcggggcg gttgtcgtcg tcgccgcggc
     2101 ggctgcctgg cgggtgctcg tggccagcga gccgtcggga cgctgcgtcg tcgtcgtgac
     2161 gcgggtggtc tcggtcgtgg tcgtggtcgt cgtgccttgc gcggcggcgc cgaacgccgt
     2221 cgcgacggcc gccgccagca cgagcgggcg aagggaggac tgcttcgtca tcggggatcc
     2281 catggaaatg aaggcggccg gcccgcggcc gctgcaagcg ccgggcggag caagcgcgac
     2341 gcctgccccc cggcggcgcg tcaccagagg cggatgcgat cggccggcgc cttccacatg
     2401 cggtcgcccg gcttggtgcc gaaggcctcg tggaagccgt cggcgttcat cgccgcgccg
     2461 tcggcgcgga agcgcgtcgg cgagtggggg tcgatcgtga ccagctgcag cgcgcggtcg
     2521 tcgcgcacct tctcgcgcca ggcctgcgac cagccgagat agaagcgctg ctcgccgctc
     2581 aggccgtcga ccggcgccgg ctccttgccg cccagcgaga gcttgtaggc cttgtaggcg
     2641 atctgcaggc ccgagaggtc ggcgatgttc tcgccgagcg tgagcttgcc gttgaggtgc
     2701 tggccgggca gcggctggta ggcggcgaac tgcgcgacca ggcgcgccgt gatgccggcg
     2761 aaggcccggc ggtcggccgc ggtccaccag ttgcgcaggc ggccgtcgcc gtcgtactgg
     2821 ctgccctcgt cgtcgaagcc gtggctgatc tcgtggccga tgatcgcgcc gatcgcgccg
     2881 tagttgaccg cgtcgtccgc ctgcatgtcg aagaacggcg gctgcaggat cgcggccggg
     2941 aaggtgatcg cgttcttgtt cggatcgtag tcggcgttga cggtctgcgg cagcaggtcc
     3001 cactcgccgc ggtcgaccgg cttgcccgag cgcacggcct ggcgcaggta gtcgaagcgc
     3061 gacgcgcgca tggcgttgcc gaacgggtcg ccggcacgga tctcgagcgc cgagtagtcg
     3121 cgccagtggt cggggtagcc gatcttgacg gcgtacttcg ccagcttctc gtgcgccgcg
     3181 gccttggtct gcggcgacat ccaggtcagc ccgtcgatcg agcccgcgta ggcggcgagc
     3241 aggttgttga ccagctcgag catgcgcgcc ttgtgcgcgg gcgggaagta ctgcgcgacg
     3301 tagagctggc cgacggcctc gccgagcgcc ccgttgaccg agtccacgcc ctgctgccag
     3361 cgcgcgcggt cttccttcag gccctggatc gcggcgccgt ggaaggcgaa gctcgcctcc
     3421 cggaagccgg ccggcagcac gtgggcgcgc gcgtcgagca ggcgcacgcg caggtactgc
     3481 ttccaggtct cgagcggctc gcgctcgacc aggcgcgcga aggccgtcgc gtacgccggc
     3541 tgcagcaccg tcagcgtcgc gggcacgggc gtctcgccgg cgtcgacgaa ggccttccag
     3601 tccacgcccg gcgccctggc cgacaactgc gccagcgtca tcgggttgta cgccttgacc
     3661 gggtcgcgca gcgcctcctg cgaccactgc gccgcggcca gcttgcgctc cagcgcgatc
     3721 acggccgccg catgcgccgc cgcctgcggg tcgccgtcca gcgccagcag ctgccgcacg
     3781 tagacggcgt aggccgcgcg cgccttcgcg aacgcggcgt ccttcttcag gtagtagtcg
     3841 cggtcgggca ggcccagtcc gccctgcgcg gcctgcggcg cgtagacgtg cggatccttc
     3901 gcgtcgggct gcacctgcag cgcgagcggc acgtcgacgt aggcctgcca gcgaccgagc
     3961 agctcgacga gctccgcccg cgtcgcgacg gcgtcgagct ggtccgtcca gggcttcagc
     4021 ggcgcgaggc cggccttgtc gatggcggcg gtgtcgaggt agccggcgta gtagtccgcg
     4081 atccgcgcct cgttggagcc ggccgcctgc ggcgcggcgg cgagcttctc gaccaggccc
     4141 ttgacgcgct cgtccgaaag gtcgcgcagc tcgatgaagg tgccgaattc ggacttgtcg
     4201 gccgggatct gcgtctgctg cagccagtgc ccgttcatcg ctgcgaacag gtcgtcctgc
     4261 gggcgcacgg ccggatccat gccggcgcga tcgaggccgc cgggcacggc ggtcgcgccg
     4321 accggcgtca tgggagagac cgcggcgtcc gcggcgaagg cgttggtcgc gcaggcgagc
     4381 gacacggcgg ccaccagggt ggcaaggcgc aaggggttca agacacgtcc tcgggatgcg
     4441 tgggggcggc accggccggc cacgcgcctt cgtgggacgc gccgcgcggc agggccagcc
     4501 cggatgatgg acgccaccgc ggacggtgga ctcggggcgg gcgcgcgaag cacgaggcga
     4561 tcgcgacaag ccggtgacgg cgccgatccg acgccgcggc tgcgatgatg ggcgtcgcgg
     4621 cccgtcggcg cccgcgcttt gcgacggacg gacgaggcga gggctcgaac ggcgggtcgt
     4681 gcgaaccacg atcggcgaag cgcgggtgaa ccctctgcgg gttgtcccgg agctcggacg
     4741 cgaccggccc gcgctcagcg gtccctcata caagcctagg catccggcct cagcgtcgcc
     4801 aggcgaccgc ccgtgcccgc tgcggcacgt cgtaggcgag gatcgccggc gcgaagcact
     4861 cggtgacgag cagcttcgcc ccgcggcggt cgaagacgga gcagcgcgcc cagacttcgc
     4921 gccggcccca ggcggcaccg gtggcatcgc gccagcggcg atgcaccttc gcggcccagc
     4981 gcgacgaggg cgcgaagcgg acgaactcga acggcgtgcg cgtggccgcg ggcaggccga
     5041 agagcatgtc ggccagcgcg cgcgtgccca ggccgcggat cgccttccac gtgccgcgcg
     5101 catgcaccgt ctgcagcacc gagcgcgcgt agacgaccgg caccccgtcc gcatgcagca
     5161 cgacctcgcg gccgtgcgcg acctggcccg cgcgcgcgcc gat
//
LOCUS       DASVLN010001003         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_268420, whole genome shotgun sequence.
ACCESSION   DASVLN010001003 DASVLN010000000
VERSION     DASVLN010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268420"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VF453_23525"
     CDS             complement(<1..1157)
                     /locus_tag="VF453_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX7690694.1"
                     /translation="MRLDKLTTAFQQALADGQSTAVARDNPYVEPAHVLAAMLAQPDG
                     PKSLLDRAGANTAGLRTAMDTLINGLPQVQGGQVQAGRDLVNLLQQAEKEAGKRGDQF
                     TSSEMFLLALADSKSDIGGIVRGHGLTRKALEAAIDAVRGGATVDSAEAEGQREALKK
                     YTLDLTERARAGKLDPVIGRDDEIRRAIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQ
                     RIIAGEVPETLKNKRVLVLDMAGLLAGAKFRGEFEERLKAVLKEVAQDEGRIILFIDE
                     LHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKYVEKDAALERRFQKVL
                     VDEPSVEATIAILRGLQEKYEVHHGVEITDPAIVAAAELSHRYITDRFLPD"
     gene            complement(1340..1897)
                     /locus_tag="VF453_23530"
     CDS             complement(1340..1897)
                     /locus_tag="VF453_23530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7690695.1"
                     /translation="MSDAGLPDDRPADPAPGAAEPEFAPVELGPDELRRALGRFVTGV
                     TIVTCRDAEGRPVGLTANSFNALSLDPPLVLWSLRRASGSIEAFSTASHFAVNVLSQD
                     QIELSRRFARPSHARFDEGIWRDGQGGAPVLAGCVAVFECRRHSHHEAGDHLLFIGEV
                     ERIGGSAEAPLVYHAGHYRGIGDRL"
BASE COUNT          243 a          673 c          682 g          300 t
ORIGIN      
        1 ttgtcgggca ggaagcggtc ggtgatgtag cggtggctca gctcggccgc ggcgacgatg
       61 gccgggtcgg tgatctccac gccgtggtgg acctcgtact tctcctgcag gccgcgcagg
      121 atggcgatgg tcgcctccac gctcggctcg tcgacgagca ccttctggaa gcggcgctcg
      181 agcgcggcgt ccttctcgac gtacttgcgg tactcgtcga gcgtggtcgc gccgatgcag
      241 tgcaactccc cgcgcgcgag cgccggcttg agcatgttgc ccgcgtcgat cgcgccctcg
      301 gccttgccgg cgccgaccat cgtgtggagc tcgtcgatga acaggatgat ccggccttcg
      361 tcctgcgcga cctccttcag gacggccttg agccgctcct cgaactcgcc tctgaacttc
      421 gcgccggcca gcagtccggc catgtcgagg acgagcacgc gcttgttctt cagcgtctcg
      481 ggcacctcgc ccgcgatgat gcgctgcgca aggccctcga cgatcgccgt cttgccgacg
      541 cccggctcgc cgatcagcac gggattgttc ttgctgcgcc gctgcagcac ctggatcgcg
      601 cggcggatct cgtcgtcgcg gccgatgacg gggtcgagct tgccggcgcg cgcgcgctcg
      661 gtgaggtcga gcgtgtactt cttcagcgcc tcgcgctggc cttcggcctc ggccgagtcg
      721 accgtcgcgc cgccgcgcac ggcgtcgatc gcggcctcga gcgccttgcg ggtcaggccg
      781 tggccgcgca cgatgccgcc gatgtcgctc ttcgaatcgg cgagcgcgag caggaacatc
      841 tcgctggagg tgaactggtc gccccgcttg ccggcctcct tctcggcctg ctgcagcagg
      901 ttgacgaggt cgcggccggc ctgcacctgg ccgccctgca cctgcggcag gccgttgatc
      961 agcgtgtcca tcgccgtgcg cagcccggcg gtgttggcgc cggcgcgatc gagcagggac
     1021 ttcgggccgt ccggctgcgc cagcatggcg gcgagcacgt gcgccggctc cacgtagggg
     1081 ttgtcgcgcg cgacagccgt gctctggcca tcggccaggg cctgctggaa cgcggtcgtg
     1141 agtttgtcga gtcgcatctt ggaaatcctt tcgaacttcg cgggaaatgg gcccgggccg
     1201 tgcgatttca agcgccgggg cggcaaccgg tgtgccgaac cggccactgc atcggcgcat
     1261 ccatgccggc gtggcgagtg tgcatgcgcc cgccgtctcg cggggcgaca tcgatcaagg
     1321 aaccgcgcga ccggccgcct cagagccggt cgccgatacc gcggtaatgc ccggcgtggt
     1381 agaccagcgg cgcctcggcc gagccgccga tgcgctcgac ctcgccgatg aacagcaggt
     1441 ggtcgccggc ctcgtggtgg ctgtggcggc ggcactcgaa gacggcgacg cagccggcca
     1501 gcaccggcgc gccgccctgg ccgtcgcgcc agatgccctc gtcgaagcgg gcgtgcgacg
     1561 gccgcgcgaa gcggcgcgac agctcgatct gatcctgcga cagcacgttg accgcgaaat
     1621 gactcgccgt gctgaacgcc tcgatggagc cgctcgcccg gcgcagcgac cacagcacga
     1681 gcggcggatc cagcgacagc gcgttgaagg agttggccgt cagcccgacc ggccggccct
     1741 cggcgtcgcg gcaggtcacg atggtcacgc ccgtgacgaa gcggccgagc gcccgccgca
     1801 gctcgtcggg gcccagctcg acgggcgcga actcgggctc ggcagcgccc ggcgcgggat
     1861 cggccgggcg atcgtcgggc agcccggcgt cgctcatg
//
LOCUS       DASVLN010001004         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_268436, whole genome shotgun sequence.
ACCESSION   DASVLN010001004 DASVLN010000000
VERSION     DASVLN010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268436"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..386)
                     /locus_tag="VF453_23535"
     CDS             complement(39..386)
                     /locus_tag="VF453_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX7690696.1"
                     /translation="MSATTCGVPAFPYDGPAGLQAPIAAALQRVVDPELAMSIVDIGL
                     VHGVEVRAQQVLVKMTMTSAACPVADLILDDAWDELARVLPGTLAVDIELVWEPPWSP
                     RCMSDRARRAMGW"
     gene            complement(383..847)
                     /locus_tag="VF453_23540"
     CDS             complement(383..847)
                     /locus_tag="VF453_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7690697.1"
                     /translation="MKLTAFTDYSLRVLIYVAACPDRRTTIAEIADAFGISHNHLMKV
                     VQTLGHHGWLATTRGRHGGLSLAVAPEAIVVGDVVRAVEGESAPVECFGRGRCAIARA
                     CRLRGVLAEAMRAFHAVLDRCTLADLVAGDDALVALLFRDRNAPAALPGAAR"
     gene            complement(870..3143)
                     /locus_tag="VF453_23545"
     CDS             complement(870..3143)
                     /locus_tag="VF453_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEX7690698.1"
                     /translation="MIRNSRLWRWLALVFVLSFGALGYLGWQIYLSAPPIPARVVSAD
                     GSVLFTGDQIRRGQQAWLAAGGQQLGTVWGHGAYVAPDWSADWLHREASELRTLRLAP
                     HAGAPAPGAEQAAAADAMLKQEMRRNTYDAIDDRVTVSAERAAAIRSVARHYEDLFGT
                     APALAALREQYAMTDGVLPSADDRRALAAFFFWSAWAATTDRPGEPGISYTSNWPHEP
                     LVGNTMTESAAMWSMASVILLLAGIAAMLWVHGGVKHEPDPAPLAADPFAGMKPTPSM
                     KATRKYFFAVVGLLLLQIGMGAITAHYAVEGQSFFGMPLAQVLPYVVSRTIHTQVGIF
                     WIATAWLATGLYIAPLLSGREPKFQKLGVDVLFWALIAIVVCSIATGWLGTMQHRGFS
                     YGFWIGNQGLEYTSMGRVWQYLLFVGLILWVVLLARALWPALAKPSESRGLIAMVFLS
                     ATCIGGFYSTSLMWDQHTHYSMIEYWRWWLVHLWVEGFFEVFATAVVALIFTKLGLVR
                     AASANRAIVAETIVFLFGGILGTLHHLYFTGAPTSVIAVGSVFSALEVVPLTLIGLEA
                     LQTYRKPDAAPWLAAWRWPVLCFVAVGFWNTVGAGLLGFAINPPASLYYVQGLNMTAA
                     HGHAALFGVYGMLGIGLMLFCLRGLYERGLHLDRLLKPAFWALNGGLAMMVFLSILPA
                     GIYQAWASIDQGMWYARSPAIVHSPVMETLVWLRVPGDILFAAGAVVLAWYALNLLRR
                     PRKPVAVAAGRLEQAAR"
     gene            3373..4869
                     /gene="nirK"
                     /locus_tag="VF453_23550"
     CDS             3373..4869
                     /gene="nirK"
                     /locus_tag="VF453_23550"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEX7690699.1"
                     /translation="MDAHSFDRIRRRLGLLLAVCLSAAAHGASAAGAAGPGDFGPPQG
                     EPIHAQLTSPPFVPPPIHRNHPARVIVDLEVVEHRMPISEGVNYTFWTFGGTVPGSFI
                     RVRQGDTVEFHLKNDPGNKMAHNIDLHGVTGPGGGAASSFTAPGHESQFTFKALNEGV
                     YVYHCATAPVGMHIANGMYGLILVEPPQGLPKVDHEYYVMQGDFYTGGRYREKGDQPF
                     DMEKAIDERPTYVLFNGSEGALTGDKALKARVGETVRLFVGNGGPNLVSSFHVIGAIF
                     DQVRFEGGTNVQRNVQTTLIPAGGAAVATFRTRVPGSYVLVDHSIFRAFNKGALAILK
                     VDGEPDKSIYSGKELDSVYLGDRSRPNLEAVAAATASAQRGTLSLQDQVKAGEALFTG
                     TCSVCHQADGAGIPGVFPPLAKSDFLAADTQRAIGIPLRGLTGKVTVNGTAYDSVMPP
                     MSQLNDDEVANILTFVLNSWGNPGGRISADDVRQVRAQAAGGAPAAAH"
BASE COUNT          819 a         1784 c         1773 g          637 t
ORIGIN      
        1 gacgagcgcg agcgccgccg ccgccagcgc cggcacggtc accagcccat ggcgcgccgg
       61 gcgcggtcgc tcatgcatcg cgggctccac ggcggctccc agacgagctc gatgtcgacg
      121 gccagggtgc ctggcagcac gcgggcgagc tcgtcccacg cgtcgtcgag gatgagatcc
      181 gccaccgggc aggcggccga cgtcatggtc atcttcacga gcacctgctg cgcgcgcacc
      241 tccacgccgt ggacgagccc gatgtcgacg atgctcatcg ccagctcggg atcgaccacg
      301 cgctgcagcg ccgcggcgat cggcgcctgc agtcccgccg gaccgtcgta cgggaaggcc
      361 gggacgccgc aggtcgtggc gctcatcgag cggctcccgg aagcgcggcg ggcgcgttcc
      421 gatcccggaa cagcagggcc accagcgcat cgtcgccggc gacgaggtcg gcgagcgtgc
      481 agcggtccag gacggcatga aacgcgcgca tcgcctcggc gagcacgccg cgcaggcggc
      541 aggcgcgggc gatcgcgcag cgcccgcggc cgaagcattc cactggcgcg gactcgccct
      601 cgaccgcgcg cacgacgtcg ccgacgacga tggcctcggg cgcgacggcc agcgacaggc
      661 ctccgtggcg gccgcgcgtg gtcgcgagcc agccgtggtg gccgagcgtc tgcacgacct
      721 tcatgaggtg gttgtgcgag atgccgaacg cgtccgcgat ctcggcgatc gtcgtgcgac
      781 ggtcggggca tgccgcgacg tagatcagca cgcgcaggct gtagtcggtg aaggcggtga
      841 gcttcatggc gtgtcggatc gccgccggtt cagcgggcgg cctgctccag gcggccggcc
      901 gcgaccgcca ccggcttgcg cggacggcgc agcaggttca gcgcgtacca ggccaggacg
      961 accgcgccgg cggcgaacag gatgtcgccg gggacgcgca gccacacgag cgtctccatc
     1021 accggcgagt gcacgatcgc gggcgagcgc gcgtaccaca tgccctggtc tatcgacgcc
     1081 caggcctggt agatgccggc gggcaggatg gacaggaaca ccatcatcgc caggccgccg
     1141 ttgagggccc agaacgccgg cttcagcagg cggtcgagat gcaggccgcg ctcgtagagc
     1201 ccgcgcaggc agaacagcat caggccgatc cccagcatgc cgtaaacccc gaacagcgcc
     1261 gcatggccat gggccgccgt catgttcagg ccctgcacgt agtaaagcga cgcgggcggg
     1321 ttgatcgcga agcccagcag gcccgcgccg acggtgttcc agaagcccac cgcgacgaag
     1381 cacagcacgg gccagcgcca cgcggcgagc cagggcgcgg cgtcgggctt gcggtaggtc
     1441 tgcagcgcct ccaggccgat gagcgtcagt ggcacgacct cgagggccga gaacaccgag
     1501 ccgaccgcga tcaccgacgt cggcgcgccg gtgaagtaca ggtggtgcag cgtgccgagg
     1561 atgccgccga acaggaacac gatggtctcg gcgacgatgg cgcggttggc gctggcggcg
     1621 cgcaccaggc cgagcttcgt gaagatcagc gcgacgaccg cggtggcgaa gacctcgaag
     1681 aagccctcga cccacaggtg caccagccac cagcgccagt actcgatcat cgagtagtgc
     1741 gtgtgctggt cccacatcag cgacgtcgag tagaagccgc cgatgcaggt ggccgacagg
     1801 aacaccatcg cgatgaggcc gcgcgattcc gacggcttcg ccagcgcggg ccagagcgcg
     1861 cgcgcgagca gcaccaccca caggatcagg ccgacgaaca gcaggtactg ccacacgcgg
     1921 cccatgctgg tgtactcgag gccctggttg ccgatccaga agccgtacga gaagccgcga
     1981 tgctgcatcg tgcccagcca tcccgtggcg atggagcaga ccacgatggc gatgagcgcc
     2041 cagaacagca cgtcgacgcc gagcttctgg aacttcggct cgcgccccga gagcaggggc
     2101 gcgatgtaca ggccggtggc gagccaggcg gtcgcgatcc agaagatgcc gacctgcgta
     2161 tggatcgtcc ggctgacgac gtagggcagc acctgcgcga gcggcatgcc gaagaacgac
     2221 tggccttcga cggcatagtg cgcggtgatc gcgcccatgc cgatctgcag cagcagcagg
     2281 ccgacgaccg cgaagaagta cttgcgcgtc gccttcatcg acggcgtcgg cttcatgccg
     2341 gcgaacggat cggccgccag cggcgccgga tcgggctcgt gcttcacgcc gccgtgaacc
     2401 cacaacatcg cggcgatccc cgcgagcagc aggatgacgc tggccatcga ccacatcgcg
     2461 gccgactcgg tcatcgtgtt gccgaccagc ggctcgtggg gccagttgct cgtgtacgag
     2521 atgcccggct cgcccgggcg gtcggtcgtc gcggcccagg ccgaccagaa gaagaacgcc
     2581 gcgagcgcgc ggcggtcgtc cgcggacggc agcacgccgt cggtcatcgc gtactgctcg
     2641 cgcagggcgg ccagcgcggg ggcggtgccg aaaaggtcct catagtgccg cgcgaccgag
     2701 cggatggccg cggcgcgttc ggccgagacg gtgacgcggt cgtcgatggc gtcgtaggtg
     2761 ttgcggcgca tctcctgctt cagcatcgca tcggccgctg cggcctgctc cgcgcccggc
     2821 gccggcgcgc cggcatgcgg cgcgaggcgc agcgtcctca gctcgctcgc ctcgcggtgc
     2881 agccagtcgg ccgaccagtc gggcgcgaca taggcgccgt ggccccacac cgtgccgagc
     2941 tgctgaccgc ccgcggccag ccaggcctgc tgtccgcggc ggatctggtc gcccgtgaac
     3001 agcaccgagc cgtcggcgct cacgacgcgc gccgggatcg gcggcgccga caggtagatc
     3061 tgccacccga ggtagccgag cgccccgaac gacaggacga agacgagcgc gagccagcgc
     3121 cacagccggg aattccggat catgtcgatc tccttcgaac gccgctgcgc gaagaggcat
     3181 cggcaatgca tcaatcctag gaacgcgccg cgcgtccacc cttgatcgtc gtcaagccga
     3241 cggggcgcgc gcggcgatcc cgcgtcgcgc gcgccgcggt cgcaaagatg cattgacgat
     3301 gctcaatggg gccacggcgc cgccgtccta acttccggat ccatcgacga caccgttttc
     3361 gaggagttca cgatggatgc ccattcattc gacaggatcc gccgccgtct cggcctgctg
     3421 ctggcggtct gcctgtccgc cgccgcgcat ggcgcatcgg ccgctggcgc cgcggggccc
     3481 ggggacttcg gcccgccgca gggcgagccc atccatgcgc agctcacgag cccgccgttc
     3541 gtgccgccgc cgatccaccg caaccatccg gcgcgcgtga tcgtcgacct cgaggtcgtg
     3601 gagcatcgca tgcccatctc cgaaggcgtc aactacacgt tctggacctt cggcggcacg
     3661 gtgccgggca gcttcatccg cgtgcggcag ggcgacacgg tggagttcca cctcaagaac
     3721 gacccgggca acaagatggc ccacaacatc gacctgcacg gcgtgaccgg gccgggcggc
     3781 ggcgcggcgt ccagcttcac ggccccgggg cacgaatcgc agttcacctt caaggcgctc
     3841 aacgagggcg tctacgtgta ccactgcgcc accgcgcccg tgggcatgca catcgccaac
     3901 gggatgtacg gcctcatcct cgtcgagccg ccgcagggcc tgcccaaggt cgaccacgag
     3961 tactacgtga tgcagggcga cttctacacc ggcggccgct accgcgagaa gggcgaccag
     4021 ccgttcgaca tggagaaggc gatcgacgag cgcccgacct acgtgctgtt caacggcagc
     4081 gagggcgcgc tgacgggcga caaggcgctc aaggcgcgcg tcggcgagac ggtgcggctg
     4141 ttcgtcggca acggcgggcc gaacctggtc tcgagcttcc acgtgatcgg cgcgatcttc
     4201 gaccaggtgc gcttcgaggg cggcaccaac gtgcaacgca atgtccagac gacgctgatc
     4261 ccggcgggcg gcgcggcggt cgcgaccttc cgcacgcggg tgccgggcag ctacgtgctc
     4321 gtcgaccact cgatcttccg cgcgttcaac aagggcgccc tggcgatcct caaggtcgac
     4381 ggcgagccgg acaagtcgat ctactcgggc aaggagctgg actcggtcta cctcggcgac
     4441 cgctcgcgcc cgaacctcga ggccgtggcc gcggccaccg cctcggcgca gcggggcacg
     4501 ctgtcgctgc aggaccaggt caaggccggc gaagcgctgt tcacgggcac ctgctcggtc
     4561 tgccaccagg ccgacggcgc cggcatcccg ggcgtcttcc cgccgctggc caagtccgac
     4621 ttcctcgccg ccgacacgca gcgcgcgatc ggcatcccgc tgcgcgggct cacgggcaag
     4681 gtcacggtca acggcacggc ctacgactcg gtcatgccgc ccatgagcca gctcaacgac
     4741 gacgaggtcg cgaacatcct gaccttcgtc ctgaacagct ggggcaaccc gggtggccgc
     4801 atctccgcgg acgacgtgcg gcaggtgcgg gcccaggccg ccggcggcgc gcctgctgcg
     4861 gcccattgag ccatgccgcg ggccatgact gcggggatcg ccctggccgg ggcgatcgcg
     4921 gcgacggggg cgagcgcggc ggactacgtg ccgctgccgg ccggcgactt cacgagcgtc
     4981 ctgccgcagg gcgcgacgtc cggctcgacc gcg
//
LOCUS       DASVLN010001005         5467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_268493, whole genome shotgun sequence.
ACCESSION   DASVLN010001005 DASVLN010000000
VERSION     DASVLN010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268493"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1324
                     /locus_tag="VF453_23555"
     CDS             209..1324
                     /locus_tag="VF453_23555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7690700.1"
                     /translation="MAAAGGWMPFDEFMAEALYAPGLGYYSGGRRIFGLGPAGGSDFV
                     TAPELSPFFGRALAVQVREALTMTGLERVFEFGAGSGALAEALLEALDADDADDDGVL
                     RPPVDYVIVELSGALRERQQQRLARFAPRVRWLDAWPERIEGVVVGNEVLDAMPVKLV
                     ARREGRWHERGVVDARVEGRASGARFAFADLDEPFALEGETQAAEALAADVFVEGTVT
                     EIHPTAEAFVASLAERLVRGVALLVDYGFPEHEYYHPQRTGGTLMCHRGHLADTDPLV
                     DVGAKDITAHVNFSGIALAAQEAGLAVLGYTSQAHFLINCGLADLLAGASVGERANAQ
                     KLINEHEMGELFKVLAVARGVEFEPVGFRVGDRTHRL"
     gene            complement(1547..4450)
                     /locus_tag="VF453_23560"
     CDS             complement(1547..4450)
                     /locus_tag="VF453_23560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7690701.1"
                     /translation="MSETPPTSPPAPPRDAAADIAQFHEAAGQRRAELQRLQQEVVLA
                     QMRLGVGQSDQLEAAGRQLVASLLQLRDEAARAANAEASAATSRRQLADLYDLSHDAL
                     VKIDDDGAVIEFNPQAERTFGWSRAEALRLHVSDLLPGDDLLVFQHMVSKLESATLGD
                     AVPRAWPSLHARRKGGEVFPVLVGLGLGKNEKPFVLVAFDDTSQRDEMTQALRDTAQT
                     HLLTLDHLLEGCQIFDAGGRCRYANPEAARQAGRPIAALVGRIVTQAPAGTESPDLVA
                     LVQRCMDDRVPLRKEVDAVSPGGLHGRFLVGVVPTSDGVTVLSVDISEQARAKEEVRV
                     ANEALEQRVIERTLELHLARQAADAANRAKSAFLAAMSHEIRTPMNGVLGMIEVLSHT
                     ELSPELRDTIRTIRTSAFALLGVVDDILDFSKIEAGKIELEREPVVLHELVESVCDTL
                     VPVAQQRNVKLRVFVDPRLPLRILADPTRLRQILMNLLNNAVKFSGGRPGKDGSVSVR
                     AEAGDSPTPTVVLRVQDNGIGMSAEAQATLFAPFTQAESSTTRRFGGSGLGLAISKRL
                     VDLMRGQIEVSSEVGVGSTFVATLPIEPADASPPSAASDLSGVACILVGSHEGDADLR
                     SYLEHAGALVTLAPDTRSAIAAAAGVSTPVFIHQASIEREAVSSHCAEFASVQGARHL
                     VLDPERRAAVCKLDRGIVAAGGLLLRRSELLAAVAIAADRIVVEDGQQGRPRDEQEPG
                     MQAIPAEQADARGRLVLVAEDDEVNQIVIRRELEMLGYRVEIAGDGQAALDMWARGSY
                     ALLLTDLHMPVLDGYALAEAIRRAEATRPSGTQRPIPIIALTADALRGDAAKAGRRHE
                     RLPDQAASIAPVEGLAREMAAGSGRGVIRAPSASAPMNPGRRATPCHEIRSPLRRGDA
                     RRPRHAARWRPARPSATSRPSPPPRTAGWRRGSRPTRSRRSWRRRGRRRWLAT"
     gene            4593..>5467
                     /locus_tag="VF453_23565"
     CDS             4593..>5467
                     /locus_tag="VF453_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7690702.1"
                     /translation="MYAQFFGLKQKPFSIAPDPRYLFMSERHREALAHLLYGVQGGGG
                     FVLLTGEIGAGKTTVCRCFLEQIPQRCNVAYIFNPKLTVMELLATVCHEFGIPVPALT
                     AAGTPTTVKDYVDALNQYLLRTHSVGQNNLLIIDEAQNLAPEVLEQLRLLTNLETSER
                     KLLQIVLIGQPELREMLARPELEQLAQRVIARFHLEALSAKETAQYIAHRLAVAGLKG
                     ALPFDAAAIARIHQLTRGVPRRINLLCDRALLGGYASGRSRVDRAIVDKAALEVFGPR
                     AVAGGMPGARPWRPLA"
BASE COUNT          783 a         1918 c         1923 g          843 t
ORIGIN      
        1 gacgagcgcg accgggcgca ccgggctggc cgcgcccgcg agctcgtagg cgcgccggtg
       61 cggcgtggcg tcggcgtggc ggtggtcgtt cgacatgatc cctacaatga cctcgatgca
      121 gcgcaatgac tcgcccggcg acgcgggcgc cccgacggat gcctccgacg ccggcgtgcc
      181 ggcgctcgtg gaacgcatcg cctcgctcat ggccgccgcg ggcggctgga tgccgttcga
      241 cgagttcatg gcggaggcgc tgtacgcgcc cggcctgggc tactacagcg gcgggcggcg
      301 catcttcggc ctggggccgg ccggcggcag cgatttcgtc accgcacccg aattgtcgcc
      361 gtttttcggc cgtgcgctgg cggtccaggt gcgcgaagcg ttgacgatga ccgggctgga
      421 gcgcgtcttc gagttcggcg ccggcagcgg cgcgctcgcc gaggcgctgc tcgaggcgct
      481 ggacgccgac gacgcggacg acgacggcgt cctgcgtccg cccgtggatt acgtgatcgt
      541 cgagctctcg ggcgcgttgc gcgagcgcca gcagcagcgc ctcgcgcgct tcgcgccgcg
      601 ggtgcgctgg ctcgacgcct ggcccgagcg catcgagggc gtcgtcgtgg gcaacgaggt
      661 gctcgacgcg atgcccgtga agctggtcgc tcgccgcgag gggcgctggc acgagcgcgg
      721 cgtcgtcgac gcccgggtcg agggccgcgc gtccggcgcg cgcttcgcct tcgccgacct
      781 cgacgagccc ttcgcgctgg agggcgagac ccaggccgcc gaagcgctgg ccgccgacgt
      841 cttcgtcgag ggcacggtca ccgagatcca cccgaccgcc gaggccttcg tcgccagcct
      901 cgccgagcgc ctcgtgcgcg gggtggcgct gctggtcgac tacggctttc ccgaacacga
      961 gtactaccac ccgcagcgca ccggcggcac cctgatgtgc caccgcggcc acctcgccga
     1021 caccgacccg ctcgtcgacg tcggcgccaa ggacatcacg gcccacgtca acttcagcgg
     1081 catcgcgctg gcggcgcagg aggcggggct ggcggtgctc ggctacacct cgcaggcgca
     1141 cttcctcatc aactgcggcc tggccgacct gctcgcgggc gccagcgtgg gcgagcgcgc
     1201 gaatgcgcag aagctcatca acgagcacga gatgggggag ctgttcaagg tgctggcggt
     1261 ggcgcgcggg gtggagttcg agccggtcgg attccgcgtg ggggaccgga cgcaccggct
     1321 ttgattcggg gtgggacggg cgccgatgcc ctgcgcggtg ctgacgctcg ggccgccgcc
     1381 gcgttccggt tcgtcgcgcg gatctgcact tcgtccgggt ggcgccgcga gtcgtcgcaa
     1441 gtcgctggga ggctggcggg caaaggacga aagtccggtc atcggatcac gcaacgcgat
     1501 ccagccggac aaccaacgca cacggagccc accatgacca agaccatcac gtcgcaagcc
     1561 atcgccgccg tcctcgccgt cgccacgacc ttcgcgaccg cgtcgggcgt gacccacgtc
     1621 gccagccggc agttcgcggc ggcggagaag gccgcgatgt cgcagacggg cgagcaggtc
     1681 gccagcgtgc agcatgtcgt ggtcgtcgcg catcgcctcg cctgagcggc gagcgtatct
     1741 cgtgacacgg cgtcgcacga cgcccgggat tcatcggcgc cgaagcgctc ggagcgcgga
     1801 tcacgccgcg tccggagccg gcagccattt ctcgagcgag tccttcaaca ggcgcaatcg
     1861 aagcggcttg gtcaggtagt cgttcatgcc ggcgcccagc cttggccgca tcgccgcgca
     1921 gcgcatcggc cgtgagggcg atgatcggga tcggccgttg cgtcccggac ggccgcgtcg
     1981 cctcggcccg gcggatcgcc tcggcgagcg cgtagccgtc cagcacgggc atgtgcaggt
     2041 cggtcaacag caatgcgtag ctgccccgcg cccacatgtc cagcgccgcc tggccgtcgc
     2101 ccgcgatctc gacacgatag ccgagcatct cgagttcgcg gcggatcacg atctggttga
     2161 cctcgtcgtc ttcggcgacc agcaccagcc ggcctcgagc gtccgcctgc tccgcgggaa
     2221 tcgcctgcat ccctggttcc tgctcgtccc ggggacggcc ctgttgtcca tcctcgacga
     2281 cgatccggtc ggcggcgatc gccaccgccg ccagcagttc cgatcgacgc agcagcaatc
     2341 cgcccgcagc gacgatgccc cgatcgagct tgcacactgc ggcccgccgc tcgggatcga
     2401 gcaccaggtg gcgcgccccc tggacgctgg cgaattccgc gcaatgcgac gacacggcct
     2461 ctctctcgat cgatgcctgg tggatgaaga ccggtgtcga cacgcctgcg gcagccgcga
     2521 tggccgagcg ggtgtccggc gcgagggtca cgagcgcgcc ggcatgttca aggtagctgc
     2581 gcaggtcggc gtcgccttcg tggctcccca cgaggatgca ggccactccg ctcagatcgc
     2641 tcgcggcgga aggcggcgag gcgtctgccg gctcgatcgg cagggtggcg acgaaggtcg
     2701 agccgacgcc gacctcgctg ctcacctcga tctgcccgcg catcaggtcg accaggcgct
     2761 tgctgatggc caggcccagg ccgctgcctc cgaagcgccg cgtggtggag gactccgcct
     2821 gggtaaacgg cgcgaacaag gtggcctggg cctctgccga catgccgatc ccgttgtcct
     2881 gcacgcggag caccacggtg ggcgtcgggc tgtcgcccgc ctctgcacgc accgacacgc
     2941 tcccgtcctt cccggggcgc ccgccactga acttcacggc gttgttcagc aggttcatca
     3001 atatctggcg caggcgcgtg gggtcggcga ggatccgaag cggaaggcgt ggatcgacga
     3061 acacgcgcag cttgacattc ctctgctgcg cgacgggaac cagggtgtca cagacgcttt
     3121 ccaccaactc gtgcaggacg accggttcgc gctcgagctc gatcttgccg gcctcgatct
     3181 tggagaaatc gaggatgtcg tcgacgacgc ccaggagggc gaaggccgag gtgcgtatcg
     3241 tccggatggt gtcgcgcagc tcgggggaca gctcggtgtg cgagagcact tcgatcatgc
     3301 cgagtacccc gttcatcggc gtcctgatct cgtggctcat cgccgccagg aaggcgctct
     3361 tggcgcgatt cgccgcgtcc gcggcctggc gcgccaggtg cagttcaagc gttcgctcga
     3421 tcacccgttg ctccagcgct tcattggcca cgcgcacttc ctccttcgca cgcgcctgct
     3481 cgctgatgtc gacgctgagg acggtcaccc cgtcggacgt cggcacgacg cccacgagga
     3541 atcggccatg gagtccgcca ggcgatacgg catcgacttc cttgcgcagc ggcacgcggt
     3601 cgtccatgca tcgctggacg agtgcgacga ggtctggcga ctcggtgccg gcaggcgcct
     3661 gggtgacgat gcgcccgaca agcgccgcga tcggtcggcc cgcctgcctc gccgcctccg
     3721 gattggcata gcgacatcgc ccgccggcgt cgaagatctg gcagccttcg agcaggtggt
     3781 caagggtcag gaggtgcgtc tgcgccgtgt ctcgaagcgc ctgcgtcatc tcgtcgcgct
     3841 ggctggtgtc gtcgaacgca accaggacga acggcttctc gttcttgccc agacccaggc
     3901 cgaccaggac gggaaagacc tctccgccct tgcggcgcgc gtgcaggctc ggccaggccc
     3961 ggggtaccgc gtcgcccagc gtcgcgctct ccagcttcga gaccatgtgc tggaatacca
     4021 ggaggtcatc gccgggaaga aggtcgctga cgtggagtcg aagcgcttct gcgcgtgacc
     4081 agccgaacgt cctctccgct tgtggattga attcgatcac ggcgccgtcg tcatcgatct
     4141 tcaccagcgc gtcgtgcgac aggtcgtaca ggtccgccaa ctgccgtcgg gatgtcgcgg
     4201 ccgacgcctc cgcgttggcg gcccgggccg cctcgtcgcg aagctggagc aacgaggcca
     4261 cgagctgccg gccggccgcc tccagctggt cgctctgccc gacgccaagc cgcatctgcg
     4321 cgagaaccac ctcctgctgg agccgttgca gttccgcacg gcgctgcccc gccgcttcgt
     4381 ggaattgcgc gatgtcggca gcggcgtcgc gcggcggcgc cggcggcgaa gtcggaggtg
     4441 tctcggacac ggaacgggcc tgtgatggct acgggaagtg ctttccttgc gcccggcgaa
     4501 accaggaagc cgcggtcgaa acgatgcgca tcgttcatgc gggcggttga gcggcgtcaa
     4561 gtgcgggccg gttctttggc tatccttgcg gcatgtacgc ccagttcttc ggcctcaagc
     4621 agaagccgtt ctccatcgcg cccgatccgc gctacctctt catgagcgag cgccatcgcg
     4681 aggcgctggc gcacctgctc tacggcgtgc agggcggcgg cgggttcgtg ctgctgacgg
     4741 gggagatcgg cgcgggcaag acgacggtct gccgctgctt cctcgagcag atcccgcagc
     4801 gctgcaacgt cgcctacatc ttcaacccga agctgacggt gatggagctg ctggccaccg
     4861 tctgccacga gttcggcatc ccggtgccgg cgctgacggc ggcgggcacg ccgaccacgg
     4921 tcaaggacta cgtcgacgcg ctcaaccagt acctgctgcg cacccactcg gtcggccaga
     4981 acaacctcct gatcatcgac gaggcgcaga acctcgcgcc ggaggtgctg gaacagctgc
     5041 gcctgctcac caacctcgag acgagcgagc gcaagctgct gcagatcgtc ctcatcggcc
     5101 agccggagct gcgcgagatg ctggcgcggc cggagctcga gcagctcgcg cagcgcgtga
     5161 tcgcacgttt ccacctcgag gcgctgtcgg ccaaggagac cgcgcagtac atcgcgcacc
     5221 ggctcgcggt agccgggctg aagggcgcgc tgccgttcga cgccgccgcg atcgcgcgca
     5281 tccaccagct cacgcgcggc gtgccgcggc ggatcaacct gctgtgcgac cgtgcgctgc
     5341 tgggcggcta tgcgagcggc aggtcgcgcg tcgaccgcgc gatcgtggac aaggcggcgc
     5401 tggaggtctt cggcccgcgc gccgtcgcgg gcggcatgcc gggggcgcgg ccgtggcgtc
     5461 cgctggc
//
LOCUS       DASVLN010001006         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_375814, whole genome shotgun sequence.
ACCESSION   DASVLN010001006 DASVLN010000000
VERSION     DASVLN010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375814"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VF453_23570"
     CDS             complement(<1..782)
                     /locus_tag="VF453_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7690703.1"
                     /translation="MTMELRPLGKTGLRIAPLVFGGNVFGWTADEARSFELLDAFVDA
                     GFNAIDTADVYSRWVPGHAGGESESIIGRWLDGKSTRRAKVLLFTKVGSDMGGDRKGL
                     GRRWIEQAVEDSLRRLRTEVIDLYQSHRPDPATPHEETLEAYRRLIAAGKVRAIGCSN
                     FDARQVGDALRVARAGGLPEYQVLQPEYNLHDRAGFDGPLRELAIGEGLGVITYFSLA
                     SGFLTGKYRSQADLGKSARGGGMGKYLTPRGRRILAALDEVAA"
     gene            complement(900..>1504)
                     /locus_tag="VF453_23575"
     CDS             complement(900..>1504)
                     /locus_tag="VF453_23575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7690704.1"
                     /translation="IESRGLPAPWWRACDVRDIAALQAAIADAAAALGDFHVLVNNVA
                     SDDRHTLESVTVDYWDGRMAINERPAFFAIQAVVPGMRRLGGGSIVNFGSTGWQTKAG
                     EYPCYAIAKSSVNGLTRGLAGPLGKDRIRINTVSPGWVMTQRQVEKWLTPEGEEAIRR
                     NQCLPDKLQPDEIAQMVLFLAADDGRACTAQEFKVDAGWN"
BASE COUNT          200 a          547 c          544 g          213 t
ORIGIN      
        1 gcggcgacct cgtcgagcgc cgcgaggatg cgccgcccgc gcggcgtcag gtacttgccc
       61 atgccgccgc cgcgcgcgct cttgccgagg tcggcctgcg agcggtactt gcccgtgagg
      121 aagcccgacg cgaggctgaa gtaggtgatg acgcccagcc cctcgccgat cgcgagctcg
      181 cgcagcgggc cgtcgaagcc ggcgcggtcg tgcaggttgt actcgggctg cagcacctgg
      241 tactcgggca gcccgccggc gcgcgcgacg cgcagcgcgt cgccaacctg gcgcgcgtcg
      301 aagttggagc agccgatcgc ccgcaccttg cccgcggcga tcaggcggcg ataggcctcc
      361 agcgtctcct cgtgcggcgt cgcggggtcg ggccggtgcg actggtagag gtcgatcacc
      421 tcggtgcgca ggcggcgcag cgagtcctcg acggcctgct cgatccagcg ccggcccagg
      481 cccttgcgat cgccgcccat gtccgagccg accttggtga acagcagcac cttcgcgcgc
      541 cgcgtcgact tgccgtcgag ccagcggccg atgatcgact ccgactcgcc gccggcgtgc
      601 ccgggcaccc agcgcgagta gacgtcggcg gtatcgatcg cgttgaagcc ggcatcgacg
      661 aaggcgtcga gcagctcgaa gctgcgcgcc tcgtcggccg tccagccgaa cacgttgccg
      721 ccgaagacca gcggcgcgat gcgcaggccg gtcttgccga ggggacgcag ttccatcgtc
      781 atcgtcgagc tcctccatgc gggatgaggg ccgactatcg cccggaacgc acgcggcccg
      841 cgcgcggcgg gccgaaatcc cttcgcaggc gcgggcacgc ggcccgcgtc gcgccgccgt
      901 caattccagc cggcgtcgac cttgaactcc tgcgcggtgc aggcgcggcc gtcgtcggcg
      961 gcgaggaaca gcaccatctg cgcgatctcg tcgggctgca gcttgtcggg caggcactgg
     1021 ttgcggcgga tcgcctcctc gccctcgggc gtcagccatt tctcgacctg gcgctgcgtc
     1081 atcacccagc ccggcgagac cgtgttgatg cggatgcggt ccttgcccag cgggcccgcg
     1141 agcccgcgcg tcaggccgtt gaccgaggac ttggcgatcg cgtagcacgg gtattcgccc
     1201 gccttggtct gccagccggt ggagccgaag ttgacgatgg agccgccgcc caggcgccgc
     1261 atgcccggca ccacggcctg gatcgcgaag aaggccgggc gctcgttgat cgccatgcgg
     1321 ccgtcccagt agtcgacggt gaccgactcg agcgtgtggc ggtcgtcgct cgcgacgttg
     1381 ttcacgagca cgtggaagtc gcccagcgcc gcggccgcgt cggcgatggc ggcctgcagc
     1441 gcggcgatgt cgcgcacgtc gcaggcgcgc caccacggcg ccggcaggcc gcgcgactcg
     1501 atcg
//
LOCUS       DASVLN010001007         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_268583, whole genome shotgun sequence.
ACCESSION   DASVLN010001007 DASVLN010000000
VERSION     DASVLN010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268583"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VF453_23580"
     CDS             complement(<1..762)
                     /locus_tag="VF453_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7690705.1"
                     /translation="MTLHLETPLLHSTAFSHATGREVWLKMEALQPSGSFKMRGIGHA
                     CETRARAGATRFMSSSGGNAGLAVAHCGRQLRVPVTVVVPESTGERAKALIAAQGAQV
                     VVHGASWAEADAHLRASLRPDDAYVHPFDDPLLWAGHASLVDEVAAKGLRPDAVVLSV
                     GGGGLMCGVIEGLRRQGWDDVPVVAVETMGADSLAQSLAAGERVTLPAITSIATSLGA
                     RQVARQAFDEARRHPVRPVVVSDAQAVDGCLAFARE"
     gene            complement(835..1137)
                     /locus_tag="VF453_23585"
     CDS             complement(835..1137)
                     /locus_tag="VF453_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7690706.1"
                     /translation="MSHARPAVPVPVHAITWQPTGQRFEARADETILKAALRALAPLR
                     SSCRNGTCRTCMCRLGAGAVVYEIEWPGLSPEERHDGFVLPCVARATTDVVLLDER"
     gene            complement(1158..>1761)
                     /locus_tag="VF453_23590"
     CDS             complement(1158..>1761)
                     /locus_tag="VF453_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7690707.1"
                     /translation="SHLLVAIGRRPNTDGLGLELAGLAPEQDGHLAVDAQLRTRAPGV
                     WALGDVNGRGAFTHTSYHDHEVVAANLLENDPRSVDERAMAYALYTDPPLARVGMNKT
                     MARASGRRVLVGRLPMSRVGRARERGETEGFMEALVDAGTRRLLGATILGIEGDEAIH
                     ALLDVMQAGLPYTAISRTMHIHPTVSELIPTMLQSLEPLA"
BASE COUNT          216 a          651 c          668 g          226 t
ORIGIN      
        1 ctcgcgcgcg aacgcgaggc agccgtcgac cgcctgcgcg tcgctcacga cgaccggccg
       61 caccggatgc cggcgcgcct cgtcgaaggc ctggcgcgcg acctgccgcg cgcccagcga
      121 ggtggcgatg ctcgtgatcg ccggcagcgt cacgcgctcg cccgcggcca gggactgcgc
      181 gagcgagtcg gcgcccatcg tctcgaccgc cacgacgggc acgtcgtccc agccctggcg
      241 gcgcaggccc tcgatcacgc cgcacatcag cccgccgccg ccgaccgaca gcacgaccgc
      301 gtccggccgc aggcccttgg ccgcgacctc gtcgaccagg ctcgcgtgcc cggcccacag
      361 cagcgggtcg tcgaacggat ggacgtaggc gtcgtccgga cgcaggctcg cgcgcaggtg
      421 ggcgtcggcc tcggcccatg aggcgccgtg cacgacgacc tgcgcgccct gcgcggcgat
      481 cagcgccttc gcgcgttcgc cggtggactc gggcacgacg accgtgaccg gcacgcgcag
      541 ctgccgcccg cagtgcgcga ccgccaggcc cgcgttgccg cccgacgagg acatgaagcg
      601 cgtcgccccc gcgcgggcgc gcgtctcgca ggcgtggccg atgccgcgca tcttgaacga
      661 gccgctgggc tgcagcgcct ccatcttgag ccagacctcg cgcccggtgg cgtgcgagaa
      721 ggcggtcgag tgcagcagcg gagtctcgag atgcagggtc atgcggcgat tatggccagc
      781 ggcaggagcg ccgcgtcgtc gcgcccggcg gcgcttcgtc ctgcggccgg cggctcagcg
      841 ctcgtcgagc agcacgacgt cggtcgtcgc ccgcgcgacg cagggcagca cgaagccgtc
      901 gtgccgctcc tcggggctga ggcccggcca ctcgatctcg tagacgaccg cgcccgcgcc
      961 caggcggcac atgcaggtgc ggcaggtgcc gttgcggcag gagctgcgca gcggcgcgag
     1021 cgcgcgcagg gcggccttca ggatggtctc gtcggcgcgc gcctcgaagc gctggcccgt
     1081 gggctgccag gtgatcgcgt ggaccggaac ggggacggca ggccgggcgt ggctcatcgc
     1141 ggcgattgtc gcgcggttca ggccagcggc tcgagcgact gcagcatcgt cgggatcaac
     1201 tcgctgacgg tcgggtggat gtgcatggtg cgcgagatcg cggtgtaggg cagtcccgcc
     1261 tgcatgacgt cgaggagggc gtggatcgcc tcgtcgccct cgatgccgag gatcgtcgcg
     1321 cccagcaggc gtcgcgtgcc ggcgtcgacc agcgcctcca tgaagccctc ggtctcgccg
     1381 cgctcgcgtg cgcggccgac gcggctcatc ggcagccgcc cgaccagcac ccgccggccc
     1441 gaggcgcgtg ccatcgtctt gttcatgccg acgcgcgcga gcggcgggtc ggtgtacagc
     1501 gcataggcca tggcgcgttc gtcgacgctg cgcgggtcgt tctccagcag gttggccgcg
     1561 acgacctcgt ggtcgtggta cgaggtgtgc gtgaaggcgc cgcgcccgtt gacgtcgccc
     1621 agcgcccaca cgcccggcgc gcgcgtgcgc agctgcgcgt cgacggcgag gtggccgtcc
     1681 tgctccggcg cgaggccggc gagctcgagc cccaggccgt cggtgttggg ccggcgcccg
     1741 atcgcgacca gcaggtggct g
//
LOCUS       DASVLN010001008         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_268602, whole genome shotgun sequence.
ACCESSION   DASVLN010001008 DASVLN010000000
VERSION     DASVLN010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268602"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..676)
                     /locus_tag="VF453_23595"
     CDS             complement(62..676)
                     /locus_tag="VF453_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7690708.1"
                     /translation="MKNEPAVVVLAAGAGSRYRGTRHKLSEPLGRDSVLVRTLRNALA
                     SELPVVLVVAETLVGEARGLVDPGDCVVVDTSSQPTWGMGDSIAAGVSIHASAGGWLV
                     LPGDMPFVQPATLRAVAEALDQQPIAYAQHHGRRGHPVGFAAELFSELVVLKGDEGAR
                     RLLARYPTAPVELDDAGVLFDIDTVDDLEAARRRLDVAPSTALR"
     gene            803..1438
                     /gene="pdxH"
                     /locus_tag="VF453_23600"
     CDS             803..1438
                     /gene="pdxH"
                     /locus_tag="VF453_23600"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX7690709.1"
                     /translation="MDDLSRQRKSYERDALDEDDAAADPLRQFSAWMDEAFAAKVPEP
                     NAMTLATVGADGRPSTRIVLIKGFDERGIVWYTNYGSRKGRELEAHPFAALQFHWVEL
                     ERVVRIEGAVERTSDAESDAYFASRPLDSRIGAWASPQSEVIESRAVLVGNAAKFAAK
                     FLMSPPRPPHWGGFRLRPDAWEFWQGRKSRLHDRLRYRLVEGAWVRERLAP"
     gene            complement(1550..2092)
                     /gene="msrA"
                     /locus_tag="VF453_23605"
     CDS             complement(1550..2092)
                     /gene="msrA"
                     /locus_tag="VF453_23605"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7690710.1"
                     /translation="MSEAIETITLAGGCFWCVEAVFEQLRGVVDVQSGYSNGHAVAPS
                     YGQVCTGRTGHAEVVRVRFDPHVLPLEDVLKVFFATHDPTTRDRQGNDVGTQYRSGIY
                     WSDESQLPVIRRVLAEANEALGGRVVTEVAKLDNYSAAEDYHQHYFANNPHQGYCAAV
                     VGPKVEKFRKVFRDRLRDAV"
BASE COUNT          320 a          817 c          826 g          332 t
ORIGIN      
        1 cgcgccgttg cgcaacgcgg cgcaccgcgc ctggctgcgc gcgaagcctg gacggcgtgg
       61 ctcagcgcag cgcggtgctg ggcgcgacgt cgagccggcg gcgcgcggcc tcgaggtcgt
      121 cgacggtgtc gatgtcgaac agcacgccgg cgtcgtcgag ctcgacgggt gcggtcggat
      181 agcgcgcgag caggcgccgc gcgccctcgt cgcccttgag cacgacgagc tcggagaaca
      241 gctccgccgc gaagccgacc ggatgcccgc gccgcccgtg gtgctgggcg taggcgatgg
      301 gctgctggtc cagcgcctcg gccaccgcgc gcagcgtcgc gggctgcacg aagggcatgt
      361 cgcccggcag cacgagccag ccgcccgcgc tcgcatggat cgacacgccg gcggcgatcg
      421 agtcgcccat gccccaggtc ggctgcgacg aggtgtcgac cacgacgcag tcgcccgggt
      481 cgaccagccc gcgcgcctcg ccgaccagcg tctcggccac gacgagcacg accggcagct
      541 cggaagccag ggcgttgcgc aaggtgcgca cgagcacgct gtcgcgtccc aacggctcgc
      601 tgagcttgtg acgcgtcccg cgatatcggc tccctgcgcc tgcagccagc acgacgacgg
      661 ccggttcatt tttcatgggc gctcatgcgc ggcctcgcga agctgccgcg catcgaattt
      721 ggtgatgtgc acgaagaatc gttgccaatg gccgtttgaa ccagtgggac gtttacctaa
      781 ggcgttcccc taaacttcgc gcatggacga tctctcccga cagcgcaaga gctacgagcg
      841 cgacgcgctc gacgaagacg acgcggcggc cgacccgctg cgccagttct ccgcatggat
      901 ggacgaggcc ttcgccgcga aggtcccgga gcccaacgcg atgacgctcg cgacggtggg
      961 cgccgatggc cgaccgtcga cgcgcatcgt gctcatcaag ggcttcgacg agcgcggcat
     1021 cgtctggtac acgaactacg ggagccgcaa gggccgcgag ctcgaggcgc atccctttgc
     1081 ggcgctgcag ttccactggg tcgagctcga gcgcgtggtg cgcatcgagg gcgcggtcga
     1141 gcgcacgagc gacgcggagt ccgacgccta cttcgcgtcg cggccgctgg actcacgcat
     1201 cggtgcgtgg gcctcgccgc agagcgaggt gatcgagtcg cgcgcggtgc tcgtgggcaa
     1261 cgcggccaag ttcgcggcga agttcctgat gagcccgccg cggccgccgc actggggcgg
     1321 cttccggctc aggcccgacg cctgggagtt ctggcagggg cgcaagtcgc gcctgcacga
     1381 ccggctgcgc taccgcctcg tcgagggtgc gtgggtgcgc gagcggctgg cgccctgacg
     1441 cggcccggcg cgcggcgcgg cgtggaatcg attccatccg caccgcgatc gcctcggtca
     1501 gcgccattcg gcgcccctgc gccagccccg acggggcgac cgcatgggct cagacggcgt
     1561 cgcgcagccg atcgcggaag accttgcgga acttctcgac cttgggcccg accaccgccg
     1621 cgcagtagcc ctggtgcggg ttgttggcga agtagtgctg gtggtagtcc tcggcggccg
     1681 agtagttgtc cagcttcgcg acctccgtga cgacgcggcc gcccaacgcc tcgttcgcct
     1741 cggccagcac gcgccggatg acgggcagct gcgactcgtc cgaccagtag atgcccgagc
     1801 ggtactgcgt gccgacgtcg ttgccctggc ggtcgcgcgt ggtcgggtcg tgggtcgcga
     1861 agaagacctt gagcacgtcc tcgagcggca gcacgtgcgg gtcgaagcgc acgcgcacga
     1921 cctcggcgtg gcccgtgcgg ccggtgcaga cctgcccgta gctcggcgcg acggcgtggc
     1981 cgttgctgta gccggactgc acgtccacga cgccgcgcag ctgctcgaac accgcctcga
     2041 cgcaccagaa gcacccgccg gccagcgtga tggtctcgat cgcttcgctc attcgtccac
     2101 tcctgtcgga tgcgcgcgcg gccccgcgcc gcgcgcaacg gtcgcacatg atgccgcgcc
     2161 ggcgcgttgc aagggcgacg ccacaatgcg cccatgctct cggactggtt gacgtcgatc
     2221 ggggcgggcg ggctcaagcc cgtgctcgcg gaattcgtgc tgccccccgt gccgctgctg
     2281 gcgctggcgc tcgtc
//
LOCUS       DASVLN010001009         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_214772, whole genome shotgun sequence.
ACCESSION   DASVLN010001009 DASVLN010000000
VERSION     DASVLN010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214772"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VF453_23610"
     CDS             complement(<1..1267)
                     /locus_tag="VF453_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7690711.1"
                     /translation="MNAPANTPAAPSAPAGNPKRRTALTAIAVAVLVVGGGYGAWYWI
                     NGRHSESTDNAYVQANVVQITAQVGGTVTAINADDTDYVKAGTPLVKLDQADARVALD
                     QAEANLAQTVRQARNLYANNSTLQAQMDAKLADVTRTSADLARAEDDVKRRQPLVGSG
                     AVGREEYDHALQQLASYKSAYTAAKSAYDAAREQLAANQTQTEGTTVADHPTVQNAAA
                     RVRAAWLDVQRTEVPAPVSGLVARRSVQVGQRVQAGVPLMSVVQLDHAWVDANFKEGQ
                     LERLRIGQPATLEADVYGKHVVYHGKVEGLGAGTGAAFSLLPAQNATGNWIKVVQRVP
                     VRISLDPNEIAKRPLSVGLSMTATVDVSNEDGSMLATAPRASSQGAVASTTVFDSLER
                     DADAEVARIVAANGGGRSAVAKAPVPALAG"
     gene            complement(1319..>2522)
                     /locus_tag="VF453_23615"
     CDS             complement(1319..>2522)
                     /locus_tag="VF453_23615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX7690712.1"
                     /translation="VADAVRGGEAPQVALDASVVRQRFTENSIYPPPYGGAVYNNGTV
                     QLGLGWDADVFGRTRNELRSALGQARAAQADADVAAQQLAVAVVRTYLQLARLESQRE
                     VANRTLQQRTQMLSLVEQRVKAGLDTVVERLEGEGAIPDTKAQIEAIDEQITLTRHQL
                     AALTAQPMDALDAASPTLAQLHAAPQPAAIGADLLARRPDIAAARARVEASGYEVQAQ
                     KAAFYPDINLSASFGVNSLSLGNLLEWPSRVWNVGPALHLPIFEGGTLRAQLRGKNAL
                     RDAAVDAYNESVIDAVHEAADAASSSASIARQQAQQSQALASAEDSYKYSQQRYAQGL
                     GNQLVVLNAETQVLAQRKADVDLRWRALDVQAQLMKALGGGWSQQSAAATAPAASAVS
                     TNDAHTPA"
BASE COUNT          272 a          885 c          967 g          398 t
ORIGIN      
        1 agccggcaag ggccggcacc ggcgccttcg cgaccgcgct gcggccgccg ccgttggccg
       61 cgacgatgcg cgcgacctcg gcatcggcgt cgcgctccag cgagtcgaac accgtggtcg
      121 acgcgacggc gccctgcgac gaggcgcgcg gcgccgtcgc gagcatgctg ccgtcttcgt
      181 tcgagacgtc gacggtcgcc gtcatcgaca ggcccacgct cagcgggcgc ttggcgatct
      241 cgttcggatc gagcgagatg cgcaccggca cgcgctgcac gaccttgatc cagttgcccg
      301 tcgcgttctg ggccggcagc agcgagaagg cggcccccgt gccggcgccc aggccctcga
      361 ccttgccgtg gtacacgacg tgcttgccgt agacgtcggc ctccagcgtc gccggctggc
      421 cgatgcgcag gcgctcgagc tggccttcct tgaagttggc gtcgacccag gcgtggtcga
      481 gctgcaccac cgacatcagc gggacgcccg cctgcacgcg ctgcccgacc tgcacgctgc
      541 ggcgcgcgac gaggccgctg accggcgccg gcacctcggt gcgttgcacg tccagccacg
      601 ccgcgcgcac gcgcgcggcg gcgttctgca cggtcgggtg gtcggcgacg gtggtgccct
      661 cggtctgcgt ctggttggcc gcgagctgct cgcgtgccgc gtcgtaggcc gacttggccg
      721 cggtgtacgc gctcttgtac gaggcgagct gctgcagcgc gtggtcgtat tcctcgcggc
      781 cgaccgcccc cgagccgacc agcggctggc ggcgcttgac gtcgtcctcg gcgcgcgcga
      841 gatcggcgga ggtgcgcgtc acgtcggcca gcttggcgtc catctgcgcc tgcagcgtcg
      901 agttgttggc gtacaggttg cgcgcctggc gcaccgtctg tgcaaggttg gcctcggcct
      961 ggtcgagcgc gacccgcgcg tcggcctggt cgagcttgac cagcggcgtg ccggccttca
     1021 cgtagtcggt gtcgtcggcg ttgatcgccg tgacggtgcc gccgacctgc gcggtgatct
     1081 gcacgacgtt ggcctgcacg taggcgttgt cggtgctctc ggagtggcgg ccgttgatcc
     1141 agtaccaggc gccgtagccg ccgccgacca cgaggacggc gaccgcgatc gcggtgagcg
     1201 ccgtgcggcg cttgggattg ccggcagggg cggagggtgc ggcgggggtg ttggcgggag
     1261 cgttcatggt gtgcgtttcg gtgtgtgttg cgggcttggg ccgggcgggg tcggcgcgtc
     1321 aggccggcgt gtgggcgtcg ttcgtggaca cggcggaggc agcgggcgcc gtcgcggcgg
     1381 cggactgctg cgaccagccg ccgcccagcg ccttcatcag ctgggcctgc acgtccagcg
     1441 cgcgccagcg caggtcgacg tcggccttgc gctgcgcgag cacctgcgtc tcggcgttga
     1501 gcacgaccag ctggttgccc aggccctggg cgtagcgctg ctgcgagtac ttgtagctgt
     1561 cctcggccga ggcgagcgcc tgcgactgct gggcctgctg gcgcgcgatc gaggccgacg
     1621 agctcgccgc gtcggcggcc tcgtgcacgg cgtcgatgac gctctcgttg taggcgtcga
     1681 ccgcggcgtc gcgcagcgcg ttcttgccgc gcagctgggc gcgcagcgtg ccgccctcga
     1741 agatcggcag gtgcagcgcg gggccgacgt tccacacgcg gctcggccat tcgagcaggt
     1801 tgcccaggct gagcgagttc accccgaagc tcgcgctgag gttgatgtcc ggatagaacg
     1861 cggccttctg cgcctgcacc tcgtagcccg aggcctcgac gcgcgcgcgc gccgccgcga
     1921 tgtcgggccg gcgcgcgagc aggtcggcgc cgatcgccgc cggctgcggc gcggcatgca
     1981 gctgcgcgag ggtcgggctc gcggcgtcga gcgcgtccat cggctgggcg gtcagcgcgg
     2041 caagctggtg gcgcgtgagc gtgatctgct cgtcgatcgc ctcgatctgc gccttcgtgt
     2101 cggggatcgc gccctcgcct tccaggcgct cgacgaccgt gtcgaggccg gccttcacgc
     2161 gctgctcgac cagcgacagc atctgcgtac gctgctgcag cgtgcggttc gcgacctcgc
     2221 gctggctctc caggcgcgcg agctgcaggt aggtgcgcac cacggcgacg gccagctgct
     2281 gcgcggcgac gtccgcgtcg gcctgcgcgg cgcgcgcttg gccgagcgcc gagcgcagct
     2341 cgttgcgggt gcggccgaag acgtcggcgt cccagcccag gcccagctgc acggtcccgt
     2401 tgttgtagac cgcgccgcca tacgggggcg ggtagatgct gttctccgtg aagcgctggc
     2461 gcacgaccga ggcgtcgagc gcgacctgcg gcgcctcgcc gccgcgcacg gcgtcggcga
     2521 cg
//
LOCUS       DASVLN010001010         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_52165, whole genome shotgun sequence.
ACCESSION   DASVLN010001010 DASVLN010000000
VERSION     DASVLN010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52165"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..673)
                     /gene="rnhB"
                     /locus_tag="VF453_23620"
     CDS             complement(32..673)
                     /gene="rnhB"
                     /locus_tag="VF453_23620"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7690713.1"
                     /translation="MPSRKSSTPEQIKLRFDDGIALVAGVDEAGRGPLAGPVVAAAVI
                     LDDQKRIKGLNDSKVLTPLQRERLFDEIRAKALAFCVAEASVEEIDTLNILHATMLAM
                     KRAVDGLRLPPQRVLVDGNRLPRWRYAAEAVVGGDASVASISAASILAKVTRDQICVE
                     HEQRWPQYGFAAHKGYSTAQHLAALDEHGPCEWHRRSFEPVRIAAARRACLEP"
     gene            complement(645..1856)
                     /gene="lpxB"
                     /locus_tag="VF453_23625"
     CDS             complement(645..1856)
                     /gene="lpxB"
                     /locus_tag="VF453_23625"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX7690714.1"
                     /translation="MSDTHADPPAAHVPRPFRPSLVVDESALRVGLVAGEASGDLLAS
                     LLLQGMKARWPAMTAAGIGGPRLVGLGFDAWWPAERLSFFGYVDVLLHLRSLFKLRDD
                     LGDRLIAERPDVFVGVDAPDFNFGLEKRLRAAGIKTVHFVCPSIWAWRGERVKTLGAA
                     ADHVLCLFPFEPDLLRAHGIRATFVGHPLAEVIPIEPPRAAARATLMLPADTEVVALL
                     PGSRRSEIAHIAPRMLRAARLMAARRPALRFVVPVAPGMRTLLEPMVREFAPNLALDL
                     LDGQSHEALAACDAAMVASGTATLEAALFKRPMVIAYALNRFNAMRMKRMRYQPWVGL
                     PNVLCRDFIVPELLQEAATPAALADATFVQLDDVAQRERVRLRFHELHHLLRRDTVRE
                     SADAIAQVLHA"
     gene            complement(1865..2653)
                     /gene="lpxA"
                     /locus_tag="VF453_23630"
     CDS             complement(1865..2653)
                     /gene="lpxA"
                     /locus_tag="VF453_23630"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX7690715.1"
                     /translation="MANIHPTAIVDPAAQLDESVTVGPYAVIGPHVRIAAGTTIGPHC
                     VVEGRTTIGRDNRFWQFSSIGAMPQDMSHKGELTELVIGDRNTVREFCTLNTGTHKEQ
                     GVTRIGSDNWIMAYVHVAHDVRIGDRTVLANGVTLAGHVHVGDWATIGGLTGVHQFVH
                     VGAHAMIGFQGHVAQDVPPFMTADGNPLALRAVNMTGLKRRDFSPERITAIRNMHRAL
                     YRKEQTLEQAKAEIEAMRGGDAGVDGDIDTMLAFIASATRGLVR"
     gene            complement(2659..3105)
                     /gene="fabZ"
                     /locus_tag="VF453_23635"
     CDS             complement(2659..3105)
                     /gene="fabZ"
                     /locus_tag="VF453_23635"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX7690716.1"
                     /translation="MLMDIHSIIKKLPHRYPFLLVDRVVEFEKDVRIKALKNVTINEP
                     FFNGHFPNRPVMPGVLMLEALAQASALLSFASEGEESDGSRLYYFAGIDGARFKRVVE
                     PGDQLVLESSIERKKASIYRYKTRATVDGELAVEAELMCTVRSVAP"
     gene            complement(3113..>3584)
                     /locus_tag="VF453_23640"
     CDS             complement(3113..>3584)
                     /locus_tag="VF453_23640"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX7690717.1"
                     /translation="GFGIAPTQGRWERIEQLGGVRIGNDVDIGANTCVDRGALDDTVL
                     EDGVKLDNQIQIGHNCHIGAHTVMAACVGIAGSTRVGRNVMIGGAAMIQGHIEIADGV
                     TVSASTMVMHSLRKKGVYTAIFPVDDHASWEKNAVTLRNLYALRERVRALEKKA"
BASE COUNT          508 a         1252 c         1290 g          534 t
ORIGIN      
        1 cgcccgcccg ccgcgagccc ggggccgcgc atcacggctc gaggcacgcg cgccgcgccg
       61 cggcgatccg caccggctcg aacgagcgcc gatgccactc gcacggcccg tgctcgtcga
      121 gcgccgccag gtgctgcgcc gtcgaatagc ccttgtgcgc cgcgaagccg tactgcggcc
      181 agcgctgctc gtgctcgacg cagatctggt cgcgcgtgac cttggcgagg atggacgccg
      241 ccgagatcga cgccaccgac gcgtcgccgc ccaccaccgc ctccgcggca tagcgccagc
      301 gcggcaggcg gttgccgtcg accagcacgc gctgcggcgg caggcgcagg ccgtcgaccg
      361 cgcgcttcat cgcgagcatc gtcgcgtgca ggatgttgag cgtgtcgatc tcctcgaccg
      421 aggcttcggc gacgcagaac gcgagcgcct tggcgcggat ctcgtcgaac aggcgctcgc
      481 gctgcagcgg cgtcagcacc ttcgagtcgt tcagcccctt gatgcgcttc tggtcgtcca
      541 ggatcaccgc cgccgccacg accgggccgg ccagcggccc gcggccggcc tcgtcgacgc
      601 cggcgaccag ggcgatgccg tcgtcaaagc gcagcttgat ctgttcaggc gtggaggact
      661 tgcgcgatgg catcggcgga ctcccgcacc gtgtcgcggc gcagcaggtg gtgcaattcg
      721 tggaatcgta gccgcacgcg ctcccgctgc gcaacgtcgt ccagctggac gaaggtcgcg
      781 tcggcgagcg ccgcgggcgt ggcggcctcc tgcagcagct ccgggacgat gaagtcgcgg
      841 cagagcacgt tgggcaggcc gacccagggc tggtagcgca tgcgcttcat gcgcatcgcg
      901 ttgaagcggt tcagggcgta ggcgatcacc atcggccgct tgaacagcgc ggcctcgagc
      961 gtggccgtgc cgctggcgac catcgccgcg tcgcatgcgg ccagcgcctc gtgcgactgg
     1021 ccgtcgagca ggtcgagggc gaggttgggc gcgaactcgc gcaccatcgg ctcgagcagc
     1081 gtgcgcatgc ccggggcgac cggcacgacg aagcgcagcg ccggacgccg cgctgccatc
     1141 aggcgcgccg ccctgagcat gcgcggcgcg atgtgcgcga tctcgctgcg gcggctgccg
     1201 ggcagcagcg cgacgacctc ggtgtccgcg ggcagcatga gcgtcgcgcg cgcggccgcg
     1261 cgcggtggct cgatcgggat cacctcggcc agcggatggc ccacgaaggt cgcgcggatg
     1321 ccgtgcgcac gcagcaggtc gggctcgaac gggaacaggc acagcacgtg gtctgcggcc
     1381 gcgcccagcg tcttcacgcg ctcgccgcgc caggcccaga tcgacgggca gacgaaatgc
     1441 accgtcttga tgccggccgc gcgcaggcgc ttctcgaggc cgaagttgaa gtcgggcgcg
     1501 tcgacgccga cgaagacgtc cgggcgctcg gcgatcaggc ggtcgccgag gtcgtcgcgc
     1561 agcttgaaca gcgagcgcag gtgcagcagc acgtcgacgt agccgaagaa cgagagccgc
     1621 tccgccggcc accaggcgtc gaagcccagg ccgaccagcc tcggcccgcc gatgcccgcg
     1681 gccgtcatcg ccggccagcg cgccttcatg ccctgcagca gcaggctcgc gagcaggtcg
     1741 cccgaggcct cgcccgcgac gaggcccacg cgcagcgcgg actcgtccac caccaggctc
     1801 gggcggaacg ggcgcggcac gtgcgccgcg ggaggatcgg cgtgcgtgtc gctcatggga
     1861 agagctagcg caccaggcct cgagtcgcac tcgcgatgaa cgcgagcatc gtgtcgatgt
     1921 cgccgtcgac gccggcatcg ccgccgcgca tcgcctcgat ctccgccttg gcctgctcca
     1981 gcgtctgttc cttgcggtaa agcgcccggt gcatgttgcg gatcgcggtg atgcgctcgg
     2041 gcgagaagtc gcgccgcttc agcccggtca tgttgacggc gcgcagcgcg agcggattgc
     2101 cgtcggcggt catgaacggc ggcacgtcct gcgcgacgtg gccctggaag ccgatcatcg
     2161 cgtgggcgcc gacgtgcacg aactggtgca cgcccgtcag gccgccgatg gtggcccagt
     2221 cgccgacgtg cacgtggccg gcgagcgtca cgccgttggc gaggaccgtg cggtcgccga
     2281 tgcgcacgtc gtgggcgacg tgcacgtagg ccatgatcca gttgtcggag ccgatgcgcg
     2341 tgacgccctg ctccttgtgc gtgccggtgt tgagggtgca gaactcgcgc accgtgttgc
     2401 ggtcgccgat caccagctcg gtcagctcgc ccttgtggct catgtcctgc ggcatcgcgc
     2461 cgatcgagct gaactgccag aagcggttgt cgcggccgat ggtggtgcgg ccctcgacca
     2521 cgcagtgcgg gccgatcgtc gtgcccgcgg cgatgcgcac gtgcggcccg atgacggcgt
     2581 acggaccgac ggtgacggac tcgtcgagct gcgccgccgg gtcgacgatc gcggtcgggt
     2641 ggatgttcgc catgggactc agggcgcgac cgagcgcacg gtgcacatca gctcggcttc
     2701 gacggccagc tcgccgtcca ccgtggcgcg cgtcttgtag cggtagatgc tcgccttctt
     2761 gcgctcgatg ctggactcga ggaccagctg gtcgcccggc tcgaccacgc gcttgaagcg
     2821 cgcgccatcg atgcccgcga agtagtacag gcgcgagccg tcgctctcct cgccctcgga
     2881 ggcgaacgac agcagcgccg aggcctgcgc cagcgcctcc agcatcagca cgccaggcat
     2941 caccgggcgg ttcgggaagt ggccgttgaa gaacggctcg ttgatcgtga cgttcttgag
     3001 cgccttgatg cgcacgtcct tctcgaactc gacgacgcgg tcgacgagca ggaaggggta
     3061 gcgatgcggc agcttcttga tgatcgagtg gatgtccatc agcatgggga actcaggctt
     3121 tcttctcgag cgcgcgcacg cgctcgcgca gggcgtacag gttgcgcagg gtgacggcgt
     3181 tcttctccca gctcgcatgg tcgtcgaccg ggaagatcgc cgtgtagacg cccttcttgc
     3241 gcagcgagtg catcaccatg gtcgatgccg agacggtcac gccgtcggcg atctcgatgt
     3301 ggccctggat catggccgcg ccgccgatca tcacgttgcg gccgacccgg gtcgagcccg
     3361 cgatgccgac gcacgccgcc atcacggtgt gggcgccgat gtggcagttg tggccgatct
     3421 ggatctggtt gtcgagcttg acgccgtcct ccagcaccgt gtcgtccagc gcgccgcggt
     3481 cgacgcaggt gttggcgccg atgtcgacgt cgttgccgat gcgcacgccg ccgagctgct
     3541 cgatgcgctc ccagcgcccc tgcgtcggcg cgatgccgaa gccg
//
LOCUS       DASVLN010001011         3475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_52519, whole genome shotgun sequence.
ACCESSION   DASVLN010001011 DASVLN010000000
VERSION     DASVLN010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52519"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VF453_23645"
     CDS             complement(<1..266)
                     /locus_tag="VF453_23645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7690718.1"
                     /translation="MNFAESQRNPKKHPTGLLIVIAMHLLLGYALVSGLGKRVVDVVK
                     VAETKVIDAPPPPPPPPPKDLPPPPKNLPPPPVYVPPPPVDVPPP"
     gene            851..1282
                     /locus_tag="VF453_23650"
     CDS             851..1282
                     /locus_tag="VF453_23650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690719.1"
                     /translation="MNKSLATLVAAALAAGVSVAAHAQATDDNSVTRSSTTSTVNADG
                     STTTTTTTTTDTIKYPPEPTTLSKSEAKDAKTLSAGDYKARKKIAEANYAENKADCKA
                     AAYGSLERACKHDAKAQEKKDKADAKLIHEAEKVDIRDNTR"
     gene            complement(1385..2641)
                     /locus_tag="VF453_23655"
     CDS             complement(1385..2641)
                     /locus_tag="VF453_23655"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEX7690720.1"
                     /translation="MNVIQRAQAILLKPREEWPVIDAEPASVASLYSGYIVILAAIPA
                     IAGFIGLSLIGAGAFGFSFKIPIVTGLVRMVTGYLLSLVSTFIGALVVDALAPSFGGV
                     KNQVAALKVVAFSSTAYWIAGVLQILPSLGIIGVLLGLYSIYLLYLGLPVVMKCPAEK
                     AAGYTAVTFVCMLVIGIVMGAIIAVLAPTGAMGLGGLGAHAAAGDGEGKFTLKTPDGQ
                     VTFDTEGMKAAAARMDAARARMDAAQAASDPAAAGKALGEMMGAMAGNGGAAPIPASD
                     LKGMLPESLGDMKRESFEANGGAAMGIATSTATATYAAGDKRVHLAITDLGGMSGLAA
                     MAGWANMTQDRETQDEVEKVYKDGNRTIHEEYRKDGSHAEYSVVLANGVIVEAKGDGV
                     DVGAVKAAAGGIDLGRIESMKRAQKA"
     gene            2921..3160
                     /locus_tag="VF453_23660"
     CDS             2921..3160
                     /locus_tag="VF453_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690721.1"
                     /translation="MNATRQPLNDDAVLQRTEAGQREALLDRHSLDDVQRRLLLLVNG
                     YTPLGGLAHRLPQAGDVHAHVAPLIDRGLVTLVDG"
BASE COUNT          541 a         1259 c         1189 g          486 t
ORIGIN      
        1 ggtggcggca cgtcgaccgg cggcggcggc acgtagacgg gcggcggcgg caggttcttg
       61 ggcggcggcg gcaggtcctt cggcggcggc gggggcggcg gcggcggggc gtcgatcacc
      121 ttggtctcgg cgaccttcac gacatcgacc acccgcttgc cgaggcccga gacgagcgcg
      181 tagcccagca gcaagtgcat ggcgatgacg atcagcaacc cggtgggatg cttcttgggg
      241 ttgcgctgcg attcagcaaa attcactcga gagtcctcca gacgaccccg ggcggggcgc
      301 tgatcttttc tggcgcgccg cagcgcagcc gcgctacgaa gtcgcgaact atagccggat
      361 ccccatcacg ggggtttgcg ggtttccctt aattttcgac accccaaagg cacttctgcc
      421 gcactgaatg tggcgagaag gccgaattac cgtgccttgt gacagtctgg ctcctggaac
      481 gaacgttcgg ctcgctagcc ggaagccgtc cgaccggatt gcaaactccg tgccgccggc
      541 ccccgccgtc caggcctgcc gggaacgcgc cgtggacgct gcgttgccgg cagcttggcg
      601 acagcttggc gacagtgcgg cggggcagcc gcgcaccgtc cgacggcgtg ccgccctgcg
      661 gtcgggcagg cgaacgaaat ccggtgcctg gggcgcaacg ccgccgatcg ttcggggaaa
      721 gcgccgacag ccgccgtcgg tgcggcgcgc gaccgggtgt aggaccggcg gaatcggtgc
      781 cggatgggcc ccgagcttgc cgaaccgggg atgtcccggg cctcgtgccc catcccaaca
      841 ggagaacagc atgaacaagt ccctcgccac cctggtcgcc gctgccctcg ccgccggcgt
      901 gagcgtcgcc gcccacgcgc aggcgaccga cgacaactcg gtcacccgct cgagcacgac
      961 ctcgacggtg aacgccgacg gctcgaccac gacgaccacc accaccacga cggacacgat
     1021 caagtacccg cccgagccga ccaccctgtc gaagtccgag gccaaggacg ccaagacgct
     1081 gtccgccggc gactacaagg cccgcaagaa gatcgccgag gccaactacg ccgagaacaa
     1141 ggccgactgc aaggccgcgg cctacggcag cctggagcgc gcctgcaagc acgacgccaa
     1201 ggcccaggag aagaaggaca aggccgacgc caagctgatc cacgaggccg agaaggtcga
     1261 catcagggac aacacccgct gatgtccgac gacgcgccgc gccgccgcga ggcggtgggg
     1321 ccgtaatgag acgagggccg cgatcgcgtg atcgcggccc tcgtcatttt cgcctgctgc
     1381 gcgttcaggc cttttgcgcg cgcttcatcg actcgatccg gcccaggtcg atgccgccgg
     1441 ccgcggcctt gacggcgccg acgtccacgc cgtcgccctt ggcctcgacg atcacgccgt
     1501 tggcgagcac cacgctgtac tcggcatggc tgccgtcctt gcggtattcc tcgtggatcg
     1561 tgcggttgcc gtccttgtag accttctcga cctcgtcctg cgtctcgcgg tcctgcgtca
     1621 tgttggccca gccggccatc gccgccaggc cggacatgcc gcccaggtcg gtgatcgcca
     1681 ggtgcacgcg cttgtcgccg gccgcgtagg tcgcggtcgc ggtggaggtc gcgatgccca
     1741 tggcggcgcc gccattggcc tcgaaggact cgcgcttcat gtcgcccagg gactcgggca
     1801 gcatgccctt gaggtcggag gccgggatcg gcgccgcgcc gccgttgccc gccatcgcgc
     1861 ccatcatctc gccgagcgcc ttgcccgccg cggccgggtc gctcgcggcc tgcgcggcgt
     1921 ccatgcgcgc gcgcgcggcg tccatgcggg cggcggcggc cttcatgccc tcggtgtcga
     1981 aggtcacctg gccgtcgggc gtcttgagcg tgaacttgcc ttcgccatcg cccgccgccg
     2041 cgtgcgcccc gagcccgccg agtcccatcg cccccgtcgg cgcgaggacg gcgatgatcg
     2101 cgcccatgac gatgccgatc accagcatgc acacgaaggt cacggccgtg tagccggccg
     2161 ccttctcggc ggggcatttc atgaccaccg gcaggccgag gtacagcagg tagatcgagt
     2221 acagccccag cagcaccccg atgatgccca gcgagggcag gatctgcagg acgccggcga
     2281 tccagtacgc ggtgctcgag aacgccacga ccttcaaggc cgcgacctgg ttcttcacgc
     2341 cgccgaagct cggcgccagc gcgtcgacga ccagcgcgcc gatgaaggtc gagacgagcg
     2401 agagcaggta gcccgtgacc atgcgcacga ggccggtgac gatggggatc ttgaaactga
     2461 agccgaaggc gcccgcgccg atgagcgaca ggccgatgaa gccggcgatc gccgggatcg
     2521 cggcgaggat gacgatgtag cccgaataga gcgaggcgac ggaggccggc tcggcgtcga
     2581 tgacgggcca ttcctcgcgc ggcttcaaga ggatcgcctg ggcacgctgg atcacgttca
     2641 tgggagctcc ttgctgtcgg tgcgagaccc tccagcctcg cttggagccg cggatcatcc
     2701 gccggtcggt cggtcggcgc catcccctgg aagggcgata cgcgacagga cgccggcctc
     2761 cgtatgcccg gatacggcac cccgtatgtg cggcgacggc gccccgctgt cggtacaaac
     2821 ccgagtgccg agctcgggga tgccgacctg tcacagttcg cgcttgtcgc gccgggaacg
     2881 ccggccgtcc agttgccgaa ccaacgacaa ggagccgaag gtgaacgcca ctcgacaacc
     2941 cctgaacgac gacgccgtcc tccaacgcac cgaggccggc cagcgcgagg cgctcctcga
     3001 ccgccactcc ctcgacgacg tccagcgccg gctcctgctg ctggtcaatg gctacacgcc
     3061 gctgggcggg ctcgcgcacc ggctgccgca ggcgggcgat gtgcacgccc acgtcgcccc
     3121 gctgatcgac cgcggactgg tgaccctggt cgacggctga ccctcgcccg cgggctcggc
     3181 cggcaaatcc cggacgacgt tgccctcgtc cgcttcgacg acacccgcgc cgcggtggcg
     3241 gcatgatccc gctgaccgcg atccgcgccg acgtcgaggc cctcgccgtt ccccggcatc
     3301 gcgcggccgg cgcgggtgcg tcgtcgcggc tgagaagccg agctcgccag ggcgtgcggg
     3361 gccgtgcatg atggcgcccg ggcgccgtgc ctgcccgcgc gttccatcgc cttcgccgcc
     3421 cgaccatgtc cgaactcgag ctcaagttcc aggtgcccgc cggccgccgc gacgc
//
LOCUS       DASVLN010001012         1923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_52780, whole genome shotgun sequence.
ACCESSION   DASVLN010001012 DASVLN010000000
VERSION     DASVLN010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52780"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1658)
                     /gene="acpA"
                     /locus_tag="VF453_23665"
     CDS             complement(39..1658)
                     /gene="acpA"
                     /locus_tag="VF453_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HEX7690722.1"
                     /translation="MDRLADATAEAGALGDRVAALANRLDLAAITNVVVIYAENRSFD
                     NVFAHFPGARGVEAGAPQRDRDGSVLEKLPPAWGGLTAAGQPVTVTQAQTTNVLPNAP
                     FQVDAEHPAWGAPALPNTILTRDLYHRFFENQMQIHGGANDGFAAWGDSGGLVMGWFD
                     GSRTALWKLAQRYTLADHFFQGAFGGSFLNHQYLVCACLPDYPDADTSPARPSISVLV
                     KDAAGQWTPELAVAPNSPRSALAGPPVFVKSGNLTPKDYLGDGSFHAVNTMQPPFQPS
                     YVPPAADDAAGLHADPLNATTLPAQTRTTIGDLLDARGIGWAWYSGGWAATSADRTRV
                     YDAATGNFQSHHQPFNYYAAFDPVAHPQARAAHLKDESDLVAAAAAGTLPAVAFYKPI
                     GEDNEHPGYASLARGDAHIADIVAKLQASPQWPHMLVVVTYDENGGQWDAVAPPRGDL
                     LGPGTRIPAVVISPFARRGYVDHTPYDTGSIARFIAHRFSLPLLDGLKLRDAALVAHG
                     APAMGDLTNALDLRAAKADAATADAARTAAR"
BASE COUNT          234 a          658 c          767 g          264 t
ORIGIN      
        1 gccgcgcgcg cgtcccggtc atgcccgccc tcgccccctc agcgtgcggc cgtcctcgcg
       61 gcatcagccg tcgcggcgtc ggccttggcg gcgcgcaggt cgagcgcgtt ggtgaggtcg
      121 cccatcgccg gcgcgccgtg cgcgacgagc gccgcgtcgc gcagcttcag gccgtccagc
      181 agcggcagcg agaagcggtg ggcgatgaag cgcgcgatcg agccggtgtc gtagggcgtg
      241 tggtcgacgt agccgcggcg cgcgaacggc gagatcacga ccgccgggat gcgcgtgccc
      301 ggcccgagca ggtcgcctcg cggcggcgcg accgcgtccc actggccgcc gttctcgtcg
      361 taggtcacga cgaccagcat gtgcggccac tgcgggctcg cctgcagctt cgcgacgatg
      421 tccgcgatgt gcgcgtcgcc gcgcgcgagg ctcgcgtagc cggggtgctc gttgtcctcg
      481 ccgatcggct tgtagaacgc gaccgccggc agcgtgcccg cggcggccgc ggcgacgagg
      541 tccgactcgt ccttgaggtg cgccgcgcgc gcctgcgggt gcgcgaccgg atcgaaggcc
      601 gcgtagtagt tgaacggctg gtgatgcgac tggaagttgc ccgtcgcggc gtcgtagacg
      661 cgggtgcggt cggccgaggt cgcggcccag ccgcccgagt accaggccca cccgatgcca
      721 cgcgcatcca gcaggtcgcc gatggtggtg cgggtctgcg ccggcagcgt ggtggcgttg
      781 agcggatcgg cgtgcaggcc cgccgcgtcg tcggccgcgg gcgggacgta gctcggctgg
      841 aatggcggct gcatcgtgtt gacggcatgg aagctgccgt cgccgaggta gtccttgggc
      901 gtcaggttgc cgctcttgac gaacaccggc ggcccggcga gcgcggagcg cggcgagttg
      961 ggagcgaccg cgagctcggg cgtccactgg ccggcggcgt ccttcacgag caccgagatc
     1021 gacggcctgg cgggcgaggt gtcggcgtcc ggatagtcgg gcaggcaggc gcagaccagg
     1081 tactggtggt tcaggaacga gccgccgaac gcgccctgga agaagtggtc ggccagggtg
     1141 tagcgctgcg cgagcttcca cagcgccgtg cggctgccgt cgaaccagcc catcaccagg
     1201 ccgccggagt cgccccaggc ggcgaacccg tcgttcgcgc cgccgtggat ctgcatctgg
     1261 ttctcgaaga agcggtggta gaggtcgcgc gtgaggatgg tgttcggcag cgccggcgcg
     1321 ccccaggcgg ggtgctcggc atcgacctgg aagggcgcgt tgggcagcac gttggtggtc
     1381 tgcgcctgcg tcacggtcac cggctggccc gccgcggtca ggccgcccca cgccggcggc
     1441 agcttctcga gcacgctgcc gtcgcggtcg cgctgcggcg cgccggcctc gacgccgcgc
     1501 gcgccgggga agtgcgcgaa gacgttgtcg aagctgcggt tctcggcgta gatgacgacg
     1561 acgttggtga tggccgccag gtcgagccgg ttggcaagcg ccgcgacgcg gtcgccgagc
     1621 gccccggcct cggcggtggc gtccgcgagg cggtccatca cggcggactg ctcgatggcg
     1681 accgcctcgg agaccgacgc atcgtcgacg gcgtcgcgcg ccaggaggtc ggatgccgcg
     1741 aggttcacgc ccctgggcgc gacaaggccg agctgcgcgt ccagctggcg ctcggccacg
     1801 atgagcggcg cgccgtcgcg gcggtggtcg tcgacgatgg actgcgcctc ggtggcaagc
     1861 ggcccgaccg cgcgcgcgcc ggtcacgccg gtgtcgggcg cgcgcagcac gaagggccgg
     1921 atc
//
LOCUS       DASVLN010001013         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_53227, whole genome shotgun sequence.
ACCESSION   DASVLN010001013 DASVLN010000000
VERSION     DASVLN010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53227"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VF453_23670"
     CDS             <1..378
                     /locus_tag="VF453_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7690723.1"
                     /translation="AVAGTLPPFEDALAGTPLAAYSRPAAKDALRRRVNDWLRATDVF
                     DAVVDFDAVLRDPAHPARLAPAFDSGDHLHPGDRGNAAMADAVDLGALLAGQAVECTA
                     TPSRLRITSAAQSARCSNTSSPR"
     gene            complement(420..836)
                     /locus_tag="VF453_23675"
     CDS             complement(420..836)
                     /locus_tag="VF453_23675"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7690724.1"
                     /translation="MPRSLQPLGLAIKRLQHRQHLALEARLGAAGVTFAQWHALRTIA
                     QHPDSSSHQLAQLTFQTDQSFGGLANRMVERGLIERVPGGGRTLLHRLTRAGEAMLER
                     GHGIADEVLAESFAPLSAAERTQLAGLLGKLLASDD"
     gene            930..1706
                     /locus_tag="VF453_23680"
     CDS             930..1706
                     /locus_tag="VF453_23680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7690725.1"
                     /translation="MTSLISPLGSQALAALDILERGDGPSLLALHGGAGPGSIAAFVE
                     SASASAAVLAPTHPGFAGTPRPEGFEQVGQLADLYAALLERRGLRDVLVVGFSMGGWI
                     AAELALRAGDRLRGLVLVDAVGLQVPGEPVADVFALAPREIAALSYADPAPFFADPAT
                     LSDERRAQMAANFRALAVYGGREMGDTSLAARLGGIATPTLVAWGEQDRIASPAYGRA
                     FAAAIPGARFETIAGCGHLPQIERPDALRRLLADFDAGLR"
     gene            complement(1708..>1943)
                     /locus_tag="VF453_23685"
     CDS             complement(1708..>1943)
                     /locus_tag="VF453_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7690726.1"
                     /translation="WRVAFTSASLAGVWAAVGAGLGVTVRTALGLPARLQVLGALPAL
                     PQVGLDLHRPDAEPSPVVARLERLLAEHVKAMG"
BASE COUNT          237 a          748 c          698 g          260 t
ORIGIN      
        1 gcggtcgccg ggacgctgcc gccgttcgag gacgcacttg ccggcacccc gctcgcggca
       61 tattcgaggc ccgccgcgaa ggacgccctg cgccgccgcg tcaacgactg gctgcgcgcg
      121 accgacgtgt tcgacgcggt cgtcgacttc gacgccgtcc tgcgcgaccc cgcgcatccg
      181 gcccgtctcg cgcccgcgtt cgacagcggc gaccacctgc atcccggcga ccgcggcaac
      241 gccgccatgg ccgacgcggt cgacctcggc gcgctgctcg caggccaggc ggtcgaatgc
      301 acggcgaccc cgagccgcct ccggatcact tcggctgcgc agtcggcccg gtgctcgaac
      361 acctcgtcgc ctcggtagcc cctgtcccgc cgcgcacgcg cctgcgcgcc tgaggcgctt
      421 cagtcgtccg acgcgagcag cttgccgagc aggccggcca gctgcgtccg ctcagccgcc
      481 gacagcggcg cgaaggactc ggccagcacc tcgtcggcga tgccgtggcc gcgctcgagc
      541 atcgcctcgc ccgcgcgcgt gaggcgatgc agcagcgtgc gcccgccgcc gggcacgcgc
      601 tcgatcagcc cgcgctcgac catgcgattg gccaggcccc cgaaggactg gtcggtctgg
      661 aacgtgagct gcgccagctg gtgggaggac gagtccggat gctgcgcgat cgtccgcagc
      721 gcgtgccatt gcgcgaaggt cacgcccgcg gcgcccaggc gcgcctcgag cgcgagatgc
      781 tggcgatgct gcaggcgctt gatggcgagg ccgagcggct ggaggctcct gggcatggaa
      841 ggcgatttcc gaagagggtg aatgggttgc gcaagcatat cagtctcttt atataatgat
      901 ccttattcac ttgacgatcg gatcccgcca tgacctccct gatctccccg ctcggcagcc
      961 aggcgctggc cgcgctcgac atcctggaac gcggcgacgg gccgtccctg ctcgcgctgc
     1021 acggcggcgc cgggccgggc tcgatcgcgg ccttcgtgga gtcggcctcg gcgagcgccg
     1081 ccgtccttgc gccgacgcac ccgggcttcg cgggcacccc gcggcccgag ggtttcgagc
     1141 aagtcggcca gctcgccgac ctctacgccg cgctgctcga gcgccgcggg ctgcgcgacg
     1201 tgctggtcgt cggcttctcg atgggcggct ggatcgcggc ggagctcgcc ctgcgcgcag
     1261 gcgaccggct gcgcgggctg gtgctggtcg acgcggtggg cctgcaggtg ccgggcgagc
     1321 cggtggccga cgtcttcgcg ctggccccgc gggagatcgc ggcgctgagc tacgccgacc
     1381 cggcgccctt cttcgccgac ccggccaccc tctccgacga gcggcgcgcg cagatggcgg
     1441 ccaacttccg cgcgctggcc gtctatggcg gccgcgaaat gggcgacacc tcgctcgccg
     1501 cgcgcctggg cgggatcgcg acgcccaccc tcgtcgcctg gggcgagcag gaccgcatcg
     1561 cgagcccggc ctacggtcgc gccttcgccg ccgcgatccc gggcgcgcgc ttcgagacga
     1621 tcgccggctg cggccatctc ccgcagatcg agcgaccgga cgcgctgcgc cgcctgctcg
     1681 cggacttcga cgccggactg cggtagctca gcccatcgcc ttcacgtgct ccgcgagcag
     1741 gcgctccagc cgcgccacga ccggcgaggg ctcggcgtcg gggcggtgga ggtcgaggcc
     1801 gacctggggc agtgccggca aggcgccgag cacctgcaat cgcgcgggca ggcccagcgc
     1861 cgttcgcacg gtgacgccca gccccgcccc gaccgcggcc cagacgccgg ccaggctggc
     1921 gctcgtgaag gcgacgcgcc acg
//
LOCUS       DASVLN010001014         5790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U3074
            k141_53307, whole genome shotgun sequence.
ACCESSION   DASVLN010001014 DASVLN010000000
VERSION     DASVLN010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959704
            Sequence Read Archive: SRR10585488
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 00:23:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,701
            Genes (coding)                    :: 4,686
            CDSs (with protein)               :: 4,686
            Genes (RNA)                       :: 43
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53307"
                     /isolate="SMAG_U3074"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="Singapore"
                     /lat_lon="1.42 N 103.7 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VF453_23690"
     CDS             <1..137
                     /locus_tag="VF453_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7690727.1"
                     /translation="GGNGYINEYPLGRLWRDAKLYEIGAGTSEIRRMLIGRELFAETM
                     "
     gene            145..792
                     /locus_tag="VF453_23695"
     CDS             145..792
                     /locus_tag="VF453_23695"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7690728.1"
                     /translation="MTAAMPIAHALLPLPVLVGERGLLRALVPSDAESLRRHADDRGV
                     AERMFDGFPHPYTLEDARAWCGRDAGSDRFGYVWGIEDGRAGGSEVVGCIGLAPDSGW
                     LRCNAEVGYWIGRAHARRGLASDALRQVVDWAFAALPELTRITAHVFASNEASQGVVR
                     KCGFVREGLLPRSAIKDGRVIDRVLWATYRPELARRDADIDAALAISAGIVMERT"
     gene            856..1584
                     /gene="can"
                     /locus_tag="VF453_23700"
     CDS             856..1584
                     /gene="can"
                     /locus_tag="VF453_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEX7690729.1"
                     /translation="MPTDDVHDPDLAQLFKNNVEWADGMERRSPGFFSRLLTQQTPQY
                     LWIGCADSRVPANELVGLLPGDVFVHRNVANVVAHSDLNALSVVQFAVDALKVKHIIV
                     VGHSKCGGVTAALNNTRVGVVDNWLRHVQDVRNAHLDFLQAVPEERRVDALVELNVLE
                     QARNVCLSTVVEDAWHRGQKVAVHGWVYGLHNGLLEDLSFTVGSMDDVAPAYQRALFA
                     LRLRHLPTAASTIGVHAGDAGRGA"
     gene            1584..3407
                     /gene="aceK"
                     /locus_tag="VF453_23705"
     CDS             1584..3407
                     /gene="aceK"
                     /locus_tag="VF453_23705"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEX7690730.1"
                     /translation="MFPRQLDPSAFPIAEAILAGFNRHYQLFTATCREAKLRFERADW
                     HGQTQAQSERIAFYDKRVGEAVDRLRDEFGAESLSADAWQHIKLHYIGLLTDHLQPEC
                     AETFFNSVTTKILHRAYFRNEFIFVRPAIATEYLENEQPGARPTYRVYYPSRETLLET
                     CMRVVTNFQLDVEFEDLRRDVDQVLAALLSNMRDTRFRANFQVQVLSSLFFRNKGAYI
                     VGKIVNGFHELPFALPILHGERGGLVVDTALIGADELEMLFSFARAYFMVDTEIPSAC
                     VQFLRSLMPRKTRAELYSQIGLQKHGKNLFYRDLLFHLRHSSDKFRIAPGIKGMVMLV
                     FDLPSFPFVFKVIKDFYPPQKDTTREQIMAKYLLVKQHDRVGRMADTLEFSDVAFPRG
                     RFEDELVAELKHFCGSLVEDDGETLVISHVYIERRMVPLNIYLQDANPRQIASAVVEY
                     GNAIKDLVAANIFPGDMLWKNFGVTRHGKVVFYDYDEIESLTDCNFRRVPAPRNEEDE
                     MSGEVWYSVGRHDVFPETFEPFLLGNPAVREVFMAHHADLLDPAYWQAQKERILAGYV
                     HDVFPYDPAKRFSAQRLHGRSPTQVLSAPEPVAAADAPSPP"
     gene            3472..4650
                     /locus_tag="VF453_23710"
     CDS             3472..4650
                     /locus_tag="VF453_23710"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7690731.1"
                     /translation="MSDSIVIVSAARTPIGGMLGDFAALSAWELGAVAIKAAVERAGV
                     PGDAVDEALIGNCLMAGQGQAPARQAVRRAGLPDSAGAVTMSKMCGSGMRTLMFAHDM
                     LLAGSASVMVAGGMESMTNAPHLMFARKGVRYGATQMFDHMALDGLEDAYDRGRAMGV
                     FAESCVAKYGFTREAQDQFAIASTTRAKAANEDGSFDWEIAPVTLAGKAGDVVVKRDE
                     QPFKAKLDKIPSLKPAFKKDGTITAANASSISDGAAALVLMRESDAAKYGARPIARIA
                     GHAVHAQAPEWFSTAPAGAIEKVLKKTGWTKQSVDLWEVNEAFAAVTMAAMTEFDLAH
                     EIVNVHGGACALGHPIGASGARIVVTLLGALRKQGKKRGVASLCIGGGEATAMAVELL
                     "
     gene            4769..5245
                     /locus_tag="VF453_23715"
     CDS             4769..5245
                     /locus_tag="VF453_23715"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7690732.1"
                     /translation="MTENAPTVPDATLVAALVRVGERIEHRACYGREDIARFAALTMD
                     ANPVHHDREAARAAGLPDVIASGQHTSALMSGLAASHFSRRDDGVAREMLCLHFNFAY
                     KAPIFADSEVALEWTVAATEWNASLAGVIAQLEGKAVVADAPAVIARGTVLVRPAR"
     gene            5275..>5790
                     /locus_tag="VF453_23720"
     CDS             5275..>5790
                     /locus_tag="VF453_23720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7690733.1"
                     /translation="MTLNRWLARPRVLLALAILAAAAVGAFQLPWSPACSGRFAQLVG
                     APPFDERPAGYTLSGVLLAFRLLGDRGLAGYEAYRAVDLLFPWLLCGLVAGALVRVGA
                     ARATAFAWLAALADTAENAAQYAILVTRDDLSPRLVPLASALTQAKFAAYALMLAAVA
                     VVGVRHLLALRA"
BASE COUNT          851 a         2124 c         1958 g          857 t
ORIGIN      
        1 tcggcggcaa cggctacatc aacgagtacc cgctcggccg cctgtggcgc gacgccaagc
       61 tctacgagat cggcgccggc acgagcgaga tccgccgcat gctgatcggc cgcgagctgt
      121 tcgccgagac gatgtgagcg cgcgatgacc gccgcgatgc cgatcgccca cgccctgctg
      181 ccgctgcccg tgctggtcgg cgagcgcggc ctgctgcgcg cgctcgtgcc gtccgacgcc
      241 gagtcgctgc gccggcatgc cgacgaccgc ggcgtcgcgg agcggatgtt cgacggcttc
      301 ccgcacccct acacgctcga ggacgcgcgc gcctggtgcg ggcgcgacgc gggctccgat
      361 cgcttcggct acgtctgggg catcgaggac gggcgagcgg gcggcagcga ggtcgtcggc
      421 tgcatcgggc tcgcgccgga ttccggctgg ctgcgctgca acgccgaggt cggctactgg
      481 atcggccgcg cccacgcgcg ccgcggcctg gcaagcgacg cgctgcgcca ggtcgtcgac
      541 tgggcgttcg cggcgctgcc ggagctcacg cgcatcacgg cgcacgtctt cgcctccaac
      601 gaggcctcgc agggcgtcgt gcgcaagtgc ggcttcgtgc gcgagggcct gctgccgcgc
      661 agcgcgatca aggacggccg cgtcatcgac cgcgtgctgt gggcgaccta ccgccccgag
      721 ctcgcgcgcc gcgacgccga catcgacgcg gcgctcgcga tcagcgccgg catcgtgatg
      781 gaacgcacct gaaaccgcgg catcctcccg gctgcctaca ctgacgggtc tccactccaa
      841 gaacaccgcc aagccatgcc taccgacgac gtccacgatc ccgatctcgc ccagctgttc
      901 aagaacaacg tcgaatgggc cgacggcatg gagaggcgct cgcccggctt cttctcgcgc
      961 ctgctgacgc agcagacgcc gcagtacctg tggatcggct gcgccgacag ccgcgtcccg
     1021 gccaacgagc tcgtcgggct gctccccggc gacgtgttcg tgcaccgcaa cgtcgccaac
     1081 gtcgtcgcgc actcggatct caacgcgctg tcggtggtcc agttcgcggt cgacgcgctg
     1141 aaggtcaagc acatcatcgt cgtcggccac tcgaagtgcg gcggcgtgac cgcggcgctc
     1201 aacaacacgc gcgtgggcgt cgtcgacaac tggctgcgcc atgtgcagga cgttcgcaac
     1261 gcccacctcg atttcctgca ggccgtgccc gaggagcgcc gcgtggatgc gctggtcgag
     1321 ctcaacgtgc tggagcaggc gcgcaacgtg tgcctgtcga cggtcgtcga ggacgcgtgg
     1381 caccgcgggc agaaggtcgc cgtccacggc tgggtctacg ggctgcacaa cgggctgctg
     1441 gaggatctca gcttcacggt cggctcgatg gacgacgtcg cgccggccta ccagcgcgcg
     1501 ttgttcgcgc tgcgcctgcg ccacctgccg accgcggcgt cgacgatcgg cgtgcacgcg
     1561 ggcgacgccg gccggggcgc ctgatgttcc cgcggcagct ggatccgagc gcgttcccga
     1621 tcgccgaggc gatcctcgcg ggcttcaacc gccactacca gctgttcacc gccacctgcc
     1681 gcgaggccaa gctgcgcttc gagcgggccg actggcacgg ccagacccag gcgcagagcg
     1741 agcgcatcgc gttctacgac aagcgcgtcg gcgaggccgt cgaccgcctg cgcgacgaat
     1801 tcggcgccga gagcctgtcg gccgacgcat ggcagcacat caagctgcac tacatcggcc
     1861 tgctcaccga ccacctgcag cccgagtgcg ccgagacctt cttcaactcg gtcacgacca
     1921 agatcctgca ccgcgcgtac ttccgcaacg agttcatctt cgtgcggccc gcgatcgcga
     1981 ccgaatacct cgagaacgag cagcccggcg cgcgcccgac ctaccgcgtc tactacccgt
     2041 cgcgcgagac gctgctcgag acctgcatgc gggtcgtgac gaacttccag ctggacgtcg
     2101 agttcgagga cctgcgccgc gacgtcgacc aggtgctcgc ggcgctgctg tccaacatgc
     2161 gcgacacgcg cttccgcgcc aacttccagg tgcaggtact gtcgtccttg ttttttcgca
     2221 acaagggcgc gtacatcgtc ggcaagatcg tcaacggctt ccacgagctg ccgttcgcgc
     2281 tgcccatcct gcacggcgag cgcggcgggc tcgtggtcga cacggcgctc atcggcgccg
     2341 acgagctcga gatgctgttc agcttcgccc gcgcctactt catggtcgac accgagatcc
     2401 cgagcgcctg cgtgcagttc ctgcgctcgc tcatgccgcg caagacgcgc gcggagctgt
     2461 actcgcagat cggcctgcag aagcacggca agaacctgtt ctaccgcgac ctgctgttcc
     2521 acctgcgcca ttcgagcgac aagttccgca tcgcgcccgg catcaagggc atggtgatgc
     2581 tcgtgttcga cctgccgtcg ttcccgttcg tcttcaaggt catcaaggac ttctacccgc
     2641 cgcagaagga cacgacccgg gagcagatca tggccaagta cctgctcgtc aagcagcacg
     2701 accgcgtcgg tcgcatggcc gacacgctgg agttctccga cgtcgccttc ccgcgcggcc
     2761 gcttcgagga cgaactggtc gccgagctga agcacttctg cggcagcctg gtcgaggacg
     2821 acggcgagac gctggtcatc agccacgtct acatcgagcg ccgcatggtg ccgctcaaca
     2881 tctacctgca ggacgcgaac ccgcgccaga tcgcgtcggc ggtcgtggag tacggcaacg
     2941 ccatcaagga tctcgtcgcg gccaacatct tccccggcga catgctgtgg aagaacttcg
     3001 gcgtgacgcg ccacggcaag gtcgtcttct acgactacga cgagatcgag tccctcaccg
     3061 actgcaactt ccggcgcgtg cccgcgccgc gcaacgagga ggacgagatg tcgggcgagg
     3121 tctggtactc ggtgggcagg cacgatgtct tccccgagac cttcgagccc ttcctgctcg
     3181 gcaacccggc ggtgcgcgag gtcttcatgg cgcaccatgc ggacctgctc gatcccgcgt
     3241 actggcaggc gcagaaggag cgcatcctcg ccggctacgt gcacgacgtc ttcccctacg
     3301 atccggccaa gcgcttcagc gcgcagcgcc tgcacggcag atcgccgacg caggtgcttt
     3361 cggcgcccga gccggtggcg gcggccgacg cgccgtcgcc tccctgagaa ccgagagcgc
     3421 gccacgaagc gcgccgtccc cgtccatccg tcattgcaag gagtgcccac catgtccgat
     3481 tccatcgtca tcgtttccgc cgcccgcacg ccgatcggcg gcatgctggg cgatttcgcc
     3541 gccctgtccg cctgggagct cggcgccgtc gcgatcaagg ccgcggtcga gcgcgcgggc
     3601 gtgcccggcg acgccgtcga cgaggccttg atcggcaact gcctgatggc cggccagggc
     3661 caggctccgg cgcgccaggc ggtgcgccgc gcggggctgc ccgactccgc gggtgccgtc
     3721 accatgagca agatgtgcgg ctcgggcatg cgcacgctga tgttcgcgca cgacatgctg
     3781 ctggccggca gcgcgagcgt gatggtcgcc ggcgggatgg agagcatgac caacgcgccg
     3841 cacctgatgt tcgcgcgcaa gggcgtgcgc tacggcgcga cgcagatgtt cgaccacatg
     3901 gcgctcgacg gcctcgagga tgcctacgac cgcggccgcg cgatgggggt gttcgccgag
     3961 tcctgcgtcg ccaagtacgg cttcacgcgc gaggcgcagg atcagttcgc catcgcctcg
     4021 accacgcgcg ccaaggccgc caacgaggac ggctccttcg actgggagat cgcgccggtc
     4081 accctcgccg gcaaggcggg cgacgtcgtc gtcaagcgcg acgagcagcc gttcaaggcc
     4141 aagctggaca agatcccgtc gctcaagccc gcgttcaaga aggacggcac gatcacggcc
     4201 gccaacgcgt cgagcatctc cgacggcgcc gcggcgctgg tgctgatgcg cgagtccgat
     4261 gccgcgaagt acggcgcccg gccgatcgcc cgcatcgccg gccacgcggt gcacgcgcag
     4321 gcgccggagt ggttctccac cgcacccgca ggcgcgatcg agaaggtcct gaagaagacc
     4381 ggctggacga agcagtcggt cgacctgtgg gaagtcaacg aggccttcgc cgcggtgacg
     4441 atggccgcga tgaccgagtt cgacctggcg cacgagatcg tcaacgtcca cggcggcgcg
     4501 tgcgcgctgg gccacccgat cggcgcctcc ggggcgcgca tcgtcgtcac cctgctcggc
     4561 gcgctgcgca agcagggcaa gaagcgcggc gtcgcgtcgc tgtgcatcgg cggcggcgag
     4621 gccaccgcga tggccgtcga gctgctctga acgccgcaga cttgcaacgg cccgtaacgt
     4681 ccggggcctt ccacggtcgg gcagcgcggc atccggcaca ctgcgtgccc ttgccgagtc
     4741 cgcctgtgag actccccgga tgactgccat gaccgagaac gccccgaccg tccccgacgc
     4801 caccctcgtc gccgccctcg tgcgggtggg cgaacgcatc gagcaccgtg cctgctacgg
     4861 ccgcgaggac atcgcgcgct tcgccgcgtt gacgatggac gcgaaccccg tccaccacga
     4921 ccgcgaggcg gcgcgcgcgg cggggctgcc cgacgtgatc gcgagcgggc agcacacctc
     4981 ggcgctcatg agcggcctgg ccgcgagcca cttctcgcgc cgcgacgacg gcgtcgcgcg
     5041 cgagatgctg tgcctgcact tcaacttcgc gtacaaggcg ccgatcttcg cggacagcga
     5101 ggtcgccctc gaatggacgg tcgccgccac cgagtggaac gccagcctgg cgggcgtgat
     5161 cgcgcagctg gagggcaaag ccgtggtcgc ggatgcaccg gcggtgatcg cccgcggcac
     5221 ggtgctggtg cgccccgccc gctgacggga cgccgcccga gcccgcgttc gcccatgacc
     5281 ctgaaccgct ggctcgcccg tccccgcgtc ctcctggcgc tcgcgatcct cgcggccgcg
     5341 gcggtcggcg cgttccagct gccgtggagc ccggcttgca gcggccgctt cgcgcagctc
     5401 gtcggcgccc cgccgttcga cgagcgcccc gccggctaca cgctctcggg cgtgctgctc
     5461 gctttccggc tgctcgggga ccgcggcctg gccggctacg aggcctatcg cgcggtcgac
     5521 ctcttgttcc cgtggctgct gtgcggcctc gtcgccggcg cgctggtgcg cgtgggcgcg
     5581 gcgcgcgcga ccgccttcgc ctggctggcg gcgctcgccg acacggccga gaacgccgcg
     5641 cagtatgcga tcctcgtcac gcgcgacgac ctctcgccgc ggctcgtgcc gctcgccagc
     5701 gcgctcacgc aggccaagtt cgcggcgtac gcgctgatgc tcgccgcggt cgccgtcgtc
     5761 ggcgtgcgcc acctgctggc gctgcgggcc
//
